LOCUS       DAOSEE010000001        60088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_330_length_60088_cov_266.450769, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000001 DAOSEE010000000
VERSION     DAOSEE010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 60088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 60088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60088
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_330_length_60088_cov_266.450769"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            403..2661
                     /locus_tag="PLI76_00005"
     CDS             403..2661
                     /locus_tag="PLI76_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming)/trehalose-phosphatase"
                     /protein_id="HOI60440.1"
                     /translation="MNRLLIVSNRLPISVTRKNGDFRLQRSVGGLATGVGSFYKAYES
                     LWVGWPGMNVQRKQEEEKDRIVDTLKREQCHPVFLSPYDIKHYYDGFCNNTLWPLFHY
                     FNLFADYDQKTWTVYQRVNERFCDAVMEVVRPDDVIWVHDYHLMLLPEMLRERLPDAE
                     IGYFHHIPFPSFEVFRGLPWRREILAGLLGADLIGFHTYGYVRHFLSSVRRILGYEHS
                     FGEVRTGTRVVRTDLFPMGIDYHRFADSAGSTPVQREIERIRQKYGKRKIILSFDRLD
                     YTKGIPLRLEAFDTLLEKKPEYQGKVSLVLVAVPSRAGVSRYQALKKRIDELVGRING
                     KYGTTDWVPVTYFYNFLPFETLVAFYSAADVALVTPLRDGMNLMAKEYVATRTDGTGV
                     LILSEMAGAAEELGEAIIVNPNDRDAVIEAIEAALAMPEKEQVERNRTMQRRLMRYDI
                     ERWVGDFLTRLGDARAIRVERSEQIVTPAIREQLVDDYAAAQDRLLLLDYDGTLVPFA
                     AKPQKAVPGETTKEVLDVLSRVPGNEVVVISGRDRYTLDAWFRGMDIGLIAEHGVWTK
                     ERSGEWRMHEGLSSDWKEEIYPLLELYTDRTPGAFIEEKDFSLVWHYRRTEPILGAQR
                     AKDLKDDLLHLTSNLNVGVMEGNKVIEIKNTVVDKGRAALNWVSQHAWDFILAIGDDR
                     TDEDLFAAMPPDAYSIKVGLAPSRARFNLVSQRDVLPLLRRCIECGREGVTRKREKPG
                     REKSLIGSTAPV"
     gene            complement(2636..4231)
                     /gene="sepS"
                     /locus_tag="PLI76_00010"
     CDS             complement(2636..4231)
                     /gene="sepS"
                     /locus_tag="PLI76_00010"
                     /EC_number="6.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043816 - phosphoserine-tRNA(Cys) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-phosphoserine--tRNA ligase"
                     /protein_id="HOI60441.1"
                     /translation="MRFDVEEFKKRAREDFEHAWHEGPSVLTPPGISGQYPRLKYTRA
                     TAHPIFEITQRLRETYLSMGFDEAMNPLIVEEADIYRQFGPEAMAVLDRVFYLGGLPR
                     PNVGIARKQIEGIEAILGRTITPPVEERLRETLHGYKKGTIDGDDLTHELAAVLEADD
                     AAVVHILDAVFPEFRALAPESSRNTLRSHMTSGWFLTLSAIWEKRHLPIRLFSVDRCF
                     RREQEEGPTRLMAYHSASCVVAGEDVTLEEGKAVSEALLSAFGFTEFRFQPDEKRSKY
                     YMPGTQTEVYARHPVLDWVEVATFGIYSPSALAEYGIGVPVMNLGLGVERLAMIAYRS
                     NDIRELTYPQFFPQTLSDRDIAGAVHLRTEPASTTGKRMAEAIRATAAEHATAPGPCA
                     FTAWKGEIAGREVEVIVEEPESNTKLCGPACANEVYVHDGSVLGVPDTEKWASVRREG
                     VSTGITYLDAVAGLAAARIEEAARCGEGTTVQVKMSKLPSDVNLRIEEYAMRYITDHN
                     KKVDLRGPVFLTVRSVIPEQSTR"
     gene            complement(4228..5121)
                     /gene="twy1"
                     /locus_tag="PLI76_00015"
     CDS             complement(4228..5121)
                     /gene="twy1"
                     /locus_tag="PLI76_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-demethylwyosine synthase TYW1"
                     /protein_id="HOI60442.1"
                     /translation="MHSEACKALRKQGYQFVSPASSAAVKPCMWNKRALRGGAMCYKA
                     QFYGIESHRCVQMTPTLRCNQQCLFCWRSFEHEVVEEEECPPEAIIESLPRLQKKALA
                     GYKVSPYVAPERFAEALRPTMVAVSLSGEPTCYSQLPNLIDGLNAGGYTTFLVSNGTR
                     PDVLARCRPYQVYVSLDAPDRETYLGLCRPREDYWDCIQESLAGLAGRRSAIRTTVVR
                     GYNDFAPEGYAAIYQDSGARFVEVKGYMYLGYSRKRLQRDQMPEHSDVRAFAEKIAEH
                     CDYTVKDESPASRVVCLERKI"
     gene            complement(5102..6769)
                     /gene="argS"
                     /locus_tag="PLI76_00020"
     CDS             complement(5102..6769)
                     /gene="argS"
                     /locus_tag="PLI76_00020"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOI60443.1"
                     /translation="MFQEKYHQVERMLRACTGEEDVLLTIGGDHADIASTVAFKLAKK
                     ERRAPQKIAADIAARLSADPDLGDIRVEAVGPYVNFRFGPSLLSEAVRAATKPGYGNL
                     PRRTERVVLEHTSANPNGPLHVGHIRNTVIGDTLVRAFRKAGYPLEAQYYLNDMGRQI
                     AIVSWGFDHLGLARGEGEKADHYVARVYVAANRAIRENPEITEEIDRLMQQVERGDAE
                     TVRKFRAAVSLCAEGIKATLAALNARHDRFVWESDFVRIGDVDRIIERVRRLPQAHED
                     EILWVDLSEQGFEKKYILRRSDGTSVYSTRDLAYHTWKARNFDRMIDILGADHKLIGA
                     QLRATLELLGEQPPDIVYFEFVSLPEGSMSTRAGKFVSADELIEEVTAKAYDEVTVRR
                     PELPEGERRAIAGSVAVAAIRYDIVKVSPEKSTVFDWREALNFERQSGPYIQYAHARA
                     CSILDKAGAVSMPAYHYETEHEVALARHLARFPVVVEQTVTELRPHLLASYARELADL
                     FNAFYHYDPVLKSEGEARNSRLALVDAVRNTLQESLATLGIDALRSM"
     gene            complement(6769..8040)
                     /gene="prf1"
                     /locus_tag="PLI76_00025"
     CDS             complement(6769..8040)
                     /gene="prf1"
                     /locus_tag="PLI76_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor aRF-1"
                     /protein_id="HOI60444.1"
                     /translation="MEETHEMDTARKRYEFKKMLERLAEKEGSGTELITLYIPPDKQI
                     YDVTAQLRDEFGQCANIKSKQTRTNVQSAISSILSRLKYYKRPPENGMAVFCGTVNIG
                     GDRTDLECEIIEPPEPLNTYLYRCSSSFELEPLQQMLGEKEVYGLIVIDRREAYIGFL
                     RGNRIEAVRGATSTVPGKQRKGGQSSVRFQRLRLIAINEFYKKVGDLANDIFLAEKDF
                     FERFKGVLIGGPTPTKEEFEAGGYLHHELQKRIIGLFDVAYTNESGLAELVENASDAL
                     KGLDIIKEKNIMNRFLQELVKDDGPAAYGEESVRKNLELGSVDTLLLSDKLRRARLKL
                     ACPNSDYTEERTISIEPGKHIKDIDFGTCPNDGASLYVAEEVDIIDELTALADQSSTT
                     VRIISDDFEEGSMLYNAFGGIAAILRYRTGY"
     gene            complement(8176..8670)
                     /locus_tag="PLI76_00030"
     CDS             complement(8176..8670)
                     /locus_tag="PLI76_00030"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOI60445.1"
                     /translation="MEIYRGKRLTVEKREITLPNGKRKERVVIHPGGAVAILPVEGDD
                     CYLIRQYRYAVDDYIFEAPAGTIDEGEEPHETARRELIEETGMKAEVFTPKGWIYPSP
                     GYTDERIWLYIAEGLSPCHDYDMDEDEVIEVVRTPLRDLAAMIADGRIVDAKTICLIC
                     RCFL"
     gene            complement(8673..9332)
                     /gene="queC"
                     /locus_tag="PLI76_00035"
     CDS             complement(8673..9332)
                     /gene="queC"
                     /locus_tag="PLI76_00035"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HOI60446.1"
                     /translation="MRAVCLLSGGMDSATLAYAAKDMGYDIIALHFAYGQRTEKRERA
                     CARTIARMLDAAEFVEIGLEHFKKIGSSSLTDESIPIDEYTEKGVPATYVPFRNANLL
                     AIATSLAEARRAEAIFIGVQTGDYPGYPDCRPEFIEAFQHAIDLGTAADPPITLMTPF
                     VRMTKADILRVGLRLGVPYEETWSCYQNDDRACGVCSSCHYRRAAFRELGLCDPIAYE
                     D"
     gene            complement(9332..9940)
                     /locus_tag="PLI76_00040"
     CDS             complement(9332..9940)
                     /locus_tag="PLI76_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOI60447.1"
                     /translation="MIVSEIFRSLQGEGRNQGRPCTFIRLAGCNLRCAWCDTSYAWEG
                     GMRMSVAEVLDRVWLLNGKQICITGGEPLLQRDEVAGLLEKFDRHGYTVEIETNGTQD
                     FRDMQAFASICMDVKCPSSGEQSDLSLLPFIGRRDCVKFVVADENDFLYARAVMARYD
                     IRGEVIITPVEGSDYRAIADYIVEENLPVRFQLQLHKILGMK"
     gene            complement(9927..10340)
                     /locus_tag="PLI76_00045"
     CDS             complement(9927..10340)
                     /locus_tag="PLI76_00045"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOI60448.1"
                     /translation="MTTRIYKEVFFEATHRLIHYRGKCFRLHGHQWRVEVWIEGTADE
                     RTGIVLDYNCIKEVVGRFDHQVILNVGDPMAACIEAFHPVVTTPGDPTSELLAGLIAD
                     MIDAEAARQGLDARVKKIRVWESTSCYAERTYDRQ"
     gene            10514..10942
                     /locus_tag="PLI76_00050"
     CDS             10514..10942
                     /locus_tag="PLI76_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOI60449.1"
                     /translation="MGNGKFAARKLKRDAKRDRWHDPVYARRQLGLDVKSDPLEGAPQ
                     ARGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGRQVTAFAVGDGAINFIDEHDEVEIEG
                     IGGRLGRSMGDIPGVRFVVTKVNNVSLSEMVIGRKEKPRR"
     gene            10948..11541
                     /locus_tag="PLI76_00055"
     CDS             10948..11541
                     /locus_tag="PLI76_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOI60450.1"
                     /translation="MEEAAEAGIQDQKLLFNRWDMSEVEIKDPSLARYVNLHAMIVPH
                     SCGKLAGQQFNKSNMLIVERLINKMMQTEHNTGKKELAIRIVKDAFEIVNKKTKKNPV
                     QVLVDAVENAGPREETVRLKYGGINVPKSVDTAPQRRVDTALRFITDGVRQASHKKKK
                     TVSEALAEELIATANGDTRSYAVSKREERERIAKAAR"
     gene            11568..13760
                     /locus_tag="PLI76_00060"
     CDS             11568..13760
                     /locus_tag="PLI76_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor EF-2"
                     /protein_id="HOI60451.1"
                     /translation="MTRRKKMVERVTDLMDKPEHIRNIGIVAHIDHGKTTLSDNLLAG
                     AGMISEELAGRQLFMDSDEEEQARGITIDASNVSMVHEYAGEEYLINMIDTPGHVDFG
                     GDVTRAMRAVDGAVVVVDAVEGTMPQTETVLRQALKEGVRPVLFINKVDRLINELKVD
                     EQEMQIRLARVIDKVNKLIKGMNEKMYNEGWKLDAAKGTVAFGSALYNWAVSVPYMKK
                     SGVSFRDVYEKCNTGDMKWLAKSSPLHAVLLDMVVKHLPNPLQAQDRRIHIIWHGDYT
                     SSEGKAMLTCDPNGPVCMMVTDITFDPHAGEVATGRLFSGTLRRGTECYIMGAAKRAN
                     RLAQVGIFMGAERIEVEELAAGNIAAVTGLKDAIVGSTVTTLVDMTPFESLKHYSEPV
                     MTVAVEAKNMKDLPKLVEVLRQVAKEDPTVQVSINEETGEHLISGMGELHLEIITGRI
                     KRDKGVDIITSPPIVVYRETITGRAGPVEGKSPNRHNRFYLELEPMNPEVVKLIQQGD
                     VSMTQQAVERRDALIAAGMDKDEAKNVKAIEGTNMFIDMTKGIQYLNETMELVLEGWR
                     EALHGGPLADELVQNLKIRLVDVKLHEDAIHRGPAQVIPAVRSAVKAGLLMGGDSLLE
                     PIQKLQITVPSDQMGAATSQIQGRRGQVFDMLSEGDTMTIVGRAPVAELFGFAGDVRS
                     ATEGRAMWSTEFAGFELVPSGIVDDVVKGIRRRKGLKEQLPRPEDYLA"
     gene            13965..14699
                     /locus_tag="PLI76_00065"
     CDS             13965..14699
                     /locus_tag="PLI76_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOI60452.1"
                     /translation="MTTADPGMQAVRASAPGRGRITACSLPAVIPVPLGPTTAFIVRD
                     RGVLLIDTGNPGDEAAILAAMRRAGIRPDDLSLILVTHGHPDHYGSADALRDRTGAPI
                     AVHAADAGAVRCGVAGRFSLARIVPHQIGFMAGKTGSIEPDIVIEGAADLAVFGIRGR
                     VIPTPGHTPGSVSVFIAGGAAIVGDLLSALLPGGRPRLPFRTGDPAAARRSIRALLAL
                     GPERIYAAHGGPWAGAEVSRWFGSEW"
     gene            complement(14805..15266)
                     /locus_tag="PLI76_00070"
     CDS             complement(14805..15266)
                     /locus_tag="PLI76_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HOI60453.1"
                     /translation="MIQVKRIHGIVIAGLLVACMLMAGCTSQPENTTVTPSPAATTAV
                     TPSPAERFAFNETDDNKAVTLPAGSEIVISLDENPTTGYSWNVTSSRGLSYVNDTFIP
                     PKTDLVGAGGVHEWQYRAAEKGSAEFSAIYMQPWMNVTGSETTFSMTFTIE"
     gene            15481..16080
                     /locus_tag="PLI76_00075"
     CDS             15481..16080
                     /locus_tag="PLI76_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60454.1"
                     /translation="MKTTSSKLLFPALIVVLGICLCMTAGCTSSSSDTTTPEAATTAP
                     AAGTTVSGAARGSVTAVPFETLITFLPAAPAGWTAEEPVGITMAVEDGQWTWSSRDYT
                     KGDAQAAIVIQDSAYYNVGQWQMWNTFTSIETTEGYYKSKTIGGHPAWEVFSKPDSYG
                     TWVGVNERFMVYVSVDGGSKQDLDAFVNAINYGGIANLK"
     gene            complement(16096..16653)
                     /locus_tag="PLI76_00080"
     CDS             complement(16096..16653)
                     /locus_tag="PLI76_00080"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOI60455.1"
                     /translation="MVDEAARRRWQHPESILEGIGLAAGATFIDVGCGDGFFALPAAR
                     IVGPRGRVCGIDIDAGALDTLKKRARGEGLENIALHREMAEEIVLCEGCADVVFFGID
                     LHDFTDPVRVLLNAKRMAAPGGVIVDLDWKKEPLPFGPPVAKKFTTEHAASLMAKAGL
                     TVAAVADSGPYHYIITAVPGPGRVR"
     gene            complement(16777..16953)
                     /locus_tag="PLI76_00085"
     CDS             complement(16777..16953)
                     /locus_tag="PLI76_00085"
                     /inference="COORDINATES: protein motif:HMM:NF018096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HOI60456.1"
                     /translation="MQNVRTFRCKDLGLACEFEEKAEGENELMKRIEGHVQTAHQMNL
                     EHPEVQERVRKAMK"
     gene            complement(17103..17813)
                     /locus_tag="PLI76_00090"
     CDS             complement(17103..17813)
                     /locus_tag="PLI76_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI60457.1"
                     /translation="MIVAENLTRVYAMGRVEVQALRGVSLEVGKGEFVGIMGASGSGK
                     STLLHILGLLDRPTSGRVTIDGTDVLALSDHRRTLFRLNRLGYVFQDYALIGELTALE
                     NVYLTSLVRGDPEEEYLRKSTAILERVGLAGRTSHRQSELSGGEQQRVAIARALVNNP
                     SILLADEPCANLDSQASKSILDLFARLNEDLDQTIVMVSHEEWHKEYFCRVVTLRDGL
                     IGDTEECRREGPPPGRTA"
     gene            complement(17817..19031)
                     /locus_tag="PLI76_00095"
     CDS             complement(17817..19031)
                     /locus_tag="PLI76_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOI60458.1"
                     /translation="MTFPMFTGLRVSAFLAWKSIERGSKGTLALTIMIIALIFVNLIF
                     LPSIISGVVRAFDDQSIDFDFGNLVIEPREGDQYIDDAANLQRKVDRIPGITGTSPRI
                     AAGVTYFYRGRNAASTLYGIKPADERAVTKIADRMVEGEFLSNGDTDAIIMGTTLAGR
                     EGEEAGGLPSLRGVVVGSPVEVVYPNGEARTYRVKGIYETQSISIDASAFVTAREMNG
                     VLGLHDQASIILVKTDVNGREAEYKTELLSYGIQEDIWTYQEKSGGFVNQAIQSFDII
                     TAISALVSLIIAVVVVFIVIFINTVNRRRQIGILKAIGISQGVIVNSYILQVLFITAV
                     GTLAGIALTAGLTIYLTAYPLAFPGGPVSPAVEASAVIQSIASLFAVSVAAGYIPAWR
                     VANEDILTAIRG"
     gene            complement(19028..20296)
                     /locus_tag="PLI76_00100"
     CDS             complement(19028..20296)
                     /locus_tag="PLI76_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein"
                     /protein_id="HOI60459.1"
                     /translation="MIQEPATAPGRSMGRRTIGWISGMFIILLALIPAAAAGAPTVIV
                     SNYTVTPAVLMPGDEGTITVVLTSTASMAPGSPLDLRVDTGGVNTSPITPDNAYIENV
                     LLRSREIEVLSGSFQDVGEIGPGQSLPITFQVRAPGEEGIYFPEVWVSVRDATGVRYP
                     IPVNVGSAYALAKKPSLRVERTVPASVDPGDSFGVTLTLYNDGEASANDISVGINASS
                     DSIASTSPESYYIPVLRPGEEAALNLSFGTDPGAPLGLLPVVATVDYRSADLAVFRQV
                     ETIGIPIVGRADMSVASIRTEPARITAGDPVGLTIRIENTGTADATSVRATIGGLSLS
                     GTKEAFLGTIEPGNDGPAVFNLQAGEEGDYPYTLAIQYTDDYGAHTTEQALNLVVAGS
                     DRAPALIIAVVAIVVVIAAALYWYRRRKEE"
     assembly_gap    20634..20733
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <20734..21015
                     /locus_tag="PLI76_00105"
     CDS             <20734..21015
                     /locus_tag="PLI76_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60460.1"
                     /translation="CWQEVFRSQAGFIFPDGILRPRYEGKEMETTQWLVTLTFRVTVG
                     GVRSKDDSVAAGLEQLKQSLNSGEDVPMEATVRRITNIVEEEPIFGVRW"
     gene            21092..21751
                     /locus_tag="PLI76_00110"
     CDS             21092..21751
                     /locus_tag="PLI76_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60461.1"
                     /translation="MHEVPDTLAIDVVLLPPGPIMEMAIGANRTLLAGNPGGGIRLDG
                     EDCLPHVSVAMLPARREDMPEIIAKMDRIVRRCSPMTMTIDAVAKHRSSTGETVSAFH
                     ILRAEILALFHKTVMNVLAPYATLPAGPPIFFEEASAPSIDCFLRFPETSAYERYSPH
                     ITLGFGDLPELLPGIDLPVRFEVTKAAICHLGSHCTCRRIIAGFDLGTGASAAHGRPI
                     R"
     gene            21968..22807
                     /locus_tag="PLI76_00115"
     CDS             21968..22807
                     /locus_tag="PLI76_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOI60462.1"
                     /translation="MKLTVLVDNATLTDRYFLAEPGLSIYLEDGATRILFDAGYSGIV
                     VSNARRMGIDLLRVGEIVLSHGHLDHTWGLDALLRLHTEAIIEGRERIEPTFIAHPDA
                     FLTRSFGGAGEIGCHLSVEELFRHGKVLLTASPLWLTENLVFLGEIKRRFDFEQMPSI
                     GYIYTPDGIRDDTVADDTALACRTPEGLVIITGCAHAGICSIVEQAREVCDEDRIADI
                     IGGFHLLNPSPGRMQGILDHLAEVRPSVLHPCHCTDLAAKIALAKVADVRDVGVGLHL
                     EYG"
     gene            complement(22808..25645)
                     /locus_tag="PLI76_00120"
     CDS             complement(22808..25645)
                     /locus_tag="PLI76_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HOI60463.1"
                     /translation="MRLFRYYPEDFGVLTVKVVHMNLAFDVYVDHTRVVSALTAETLD
                     APIASLALNARDLDILRVACDGHSIAHAYDRGAAILTVTFDPPVPPRTRFTLDTETIC
                     RPSSHTLEGLYYDGSCPGGPPVQITQCQQWGFQRLVPCLDDMTAKCTYTTTIAADDGY
                     TNILSNGDPAGPRYLVAPGRSVQEYRNTTTPMAPYLFFLGVGCYDTHRREFEYPDGRA
                     FSLEILAKPGFAPGEPPERALDILADAVMWVHLFTGPEPYRDRKTRQEIWRLIRVRDA
                     AKRAGDRESLVRTREALAGLVATVTPGYAYTGSVYREIAMQNSDFGGMENVGNTTIAA
                     NRIMPYPEATDPAFEYMVRVKVHEYYHNENGSEVTGRSPFEIWLNEAVTVHIENRHHA
                     FFFGEAYSRLQTVLDLLDPVAGTLALDRGAGSMPIEPDGFDDPNDLITGVTYVKAPEF
                     VRMIETLMGKETFAVGLALYHDRFRHKNASTDDWIRCMEEVSGQDFTGMAAVWLKEKE
                     YPVLTVAPSYDPAARRFTLACRQNRSPGGRPREFPFRFALADADGRDIAERTVRIAGE
                     EEEVVIDDVDRPAFLSLNRGYSFYGKVVYRPPEDELYLQAAKDADIVGRFTAFNALAG
                     HEMLRLLEDPGAAVSSRFADLCAGLLADDALMEEAGGQFLTIFPAVDDERFAHRYRAL
                     HDARKKILAAVGARHADTLLAVYRQAAVDLPSGDGYLEDEARAIRRRQRKNTCLAILA
                     ARDAPEIHRLLAGQFREAAAATDRLTAFGLILESSAPERLNILAEFCAASAKNPVAWE
                     SFLAVVAGSDCDDIVEILRRIESSPAFSVEQAGEQRALYGRFALNRKRSLETEEGRAY
                     LGEVLRRLARVNEYSTARALDAFAFIDAMEPRHRVPVAGVLVGLLASLDPGEHPAIFN
                     NARRFLLGAPEAVRAYEAEHGEIPALRKDTS"
     gene            complement(25654..26184)
                     /locus_tag="PLI76_00125"
     CDS             complement(25654..26184)
                     /locus_tag="PLI76_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOI60464.1"
                     /translation="MERSVKRLMEDREKRREENIGKYIEQLCDESTPYRLRAAEALGG
                     CADTRAVEPLIAALHDPENEVRWVAAQALGKLCDARAVEPLLPLLADPDRWVRRGTAW
                     SLGEIGDPCAVEPLILLLTDSRKDVRIAVADALGKLHDARAVGPLTAALECEEEPEVR
                     TAIRRALRDITGEMEF"
     gene            complement(26252..26605)
                     /locus_tag="PLI76_00130"
     CDS             complement(26252..26605)
                     /locus_tag="PLI76_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60465.1"
                     /translation="MGSEETDTVAQEIMATLDTLFLAEKRARLQVSALEDRQYALATT
                     FRMVQEMEADSAIEEALSGFGFGYYTIDDDAELWISEEYGLMVFLSFTAPDGRYYHYR
                     IVSFDVIGGDGEEAG"
     gene            26773..27258
                     /locus_tag="PLI76_00135"
     CDS             26773..27258
                     /locus_tag="PLI76_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOI60466.1"
                     /translation="MAEERLKIVIFGSFNAGKSSLIQALDPRSRHVEAASDEGPTTVA
                     FDFGRLQVRDRAVYLFGTPGQERFEFVRQILSRGMDGAIIVVDATTGVDAMTRHLYNQ
                     LKDLGVPLAFMANKCDRPDAVPEAVRRELPGEMVHPISARNGENVHAALDAFVATLVA
                     R"
     gene            27293..28120
                     /locus_tag="PLI76_00140"
     CDS             27293..28120
                     /locus_tag="PLI76_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60467.1"
                     /translation="MRSLTAKETYIRDVARLLSGFIASGNTTDLLGYLTAEGGLPGAD
                     PELTGAFTGVVREFAAADPDDRRLLWDLCIMLADIPPEGAPASDLHELLISCGIRGIG
                     TIGSLSPACAEPALTRLAEASADPRQRIREAAGMGVRDILSRQRDTVIPELARWVEGG
                     SWLTMRAAIMGIVEPDLLGEPDIPTAAFHLHRKVLIRIYTAKERQSEAFGALRATLGS
                     TLALVIAALPGIGFEYLRQLATLDDPDIRWIVRENLRESGLQKRYPETVRHIGAQIG"
     gene            complement(28158..28772)
                     /locus_tag="PLI76_00145"
     CDS             complement(28158..28772)
                     /locus_tag="PLI76_00145"
                     /inference="COORDINATES: protein motif:HMM:NF018933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOI60468.1"
                     /translation="MPFIRGSPPPGDWIGVDLNATGSIAVVAHPASGGVVMLGEQPEG
                     AGTPERCSGGKRKALRAARDRESSARGDLNRRIAREIVKMARNLECGIKLEDLTGAGF
                     AGWQKPGAPLLFSRRDGSFFHLQNLLMVRAHDAGVVIALVDPALTSRCCSRCGEQGIR
                     RGKRFTCPRCGYTDHADVNAAFNIAAAPGIGRPAIPASSHRQRG"
     gene            complement(28867..29529)
                     /locus_tag="PLI76_00150"
     CDS             complement(28867..29529)
                     /locus_tag="PLI76_00150"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOI60469.1"
                     /translation="MSLCDPPVAGVLFDCYGTLIDILTDERDTETYRNLARWLIYQGV
                     MVAPDDLRVLYTCHVREAIEQIGEPYPEVRVEEIFAGICAEHAIRKIDAGRLGVETAR
                     AFRAASLRRLGIIEESRRLLDLFCAEKLGVVSNGQRVFSECEMRAFGLYDRFDFVIFS
                     SDLGYQKPDTRIYAAALGRMRLSAPEVLFIGDNPENDVAAPRKFGMQALHVEEAWRRY
                     SD"
     gene            complement(29505..30416)
                     /locus_tag="PLI76_00155"
     CDS             complement(29505..30416)
                     /locus_tag="PLI76_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOI60470.1"
                     /translation="MGRLEEGDAFRDWLAGILADRLTGWRGKIRVYRIEPASHVVCRY
                     EFAGGPSVVGKFFGAPTGARTRYNTDAAMKNEFLRLRRVSGWIRVPHAIATNNRFHSV
                     LVTEHVPGPTVRELLGAGVSLYDPLTGVAHLLRRLHDSTRTSCKRERDFAYYHAILDQ
                     NNLSGSRRKRFDRLLGAWWHSARLGPEGCMVHGDATLTNYLFNGDTCAIDFEGSRNHT
                     HPIRDLGILAAELRTSGASISTAEDYIGHLLWHYSRGEEEFRRYTAVLPFFMALGYLR
                     IARLPWRAAERDWLLEEAEACLCAIHR"
     gene            30589..31521
                     /locus_tag="PLI76_00160"
     CDS             30589..31521
                     /locus_tag="PLI76_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOI60471.1"
                     /translation="MMPFYEFLEEIRPAVNRRIGEVTGDEGSIDPGVVPLLLRGKRMR
                     AGLLLHTHAALTGRKAPTRRALDLACAVELAHAASLILDDMLDGDILRRGLPSHHLTR
                     GTGRAVLDAVGVLALPYALAAPCGAEYVAMLASAQQSMARGAAWEMLGGSGLSSIELY
                     DAIIARKTGCLFALAAAWGAMAAGEDEDVVAAFADFGLFTGKAMQIADDIADLHAPAA
                     GVPGRRPGSEVLLLQGIAGEDRTDQALAARLDTEIARAADRLGDGARQRLTPGEREAF
                     GQAVRDIVGLTIAGGLPPGTATFQVVRPGALVYR"
     gene            31602..31787
                     /locus_tag="PLI76_00165"
     CDS             31602..31787
                     /locus_tag="PLI76_00165"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HOI60472.1"
                     /translation="MPVVTIRMAKGRTLEQKRRLAAEITAAITETLGVDPERVTLFFE
                     ELDTENIARAGRLLAES"
     gene            31842..32189
                     /locus_tag="PLI76_00170"
     CDS             31842..32189
                     /locus_tag="PLI76_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60473.1"
                     /translation="MARKFDERDFAILRKLAPEYSGILCPESGHEFHSILPPVSNHIA
                     ADEEDFAERVGRLSDEDWIYLTDRILSGREDLGCMPEEDIETILIRIEKAVSDEAAER
                     IRRLYRLSGCGVL"
     gene            complement(32478..33734)
                     /locus_tag="PLI76_00175"
     CDS             complement(32478..33734)
                     /locus_tag="PLI76_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOI60474.1"
                     /translation="MGGERMPVRGIPGQAALILILVAFLVFVTGCVGAGQSGGIPDSG
                     ADTAGYRTVVDSRGVEVQVPKEIDRVVTVSSGLIEEVMFVLGEEEKTVGVGGINIQNV
                     WVYSYPLDDGKFVNGSDGRNVLQVLYPPIKELPLIMGADAAINYETLAGLDPDVVILR
                     VGTGGLTSMENEGVQKTIQTIESLRIPIVVLKGPPCFDEPDIATISDEIRIIGSVFGK
                     EERADKLADYLESQTQVVFERTKDIPDAERPTVLVFGMNAHRAGALGTQGAGSVKGTD
                     TVESYFIEKIVHAKNAYRNAGSPLISAEQLLALDPDAIVLSTSYGYHPPGELYDAMYY
                     KNVGELSAVKNHRVMSLPWTPYYSNRLEYPIDVMVIAKAAYPGRFADIDLGEWLLDFY
                     KNVYGVDDGTAKALRSAQWMDWCVEV"
     gene            complement(33710..34441)
                     /locus_tag="PLI76_00180"
     CDS             complement(33710..34441)
                     /locus_tag="PLI76_00180"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOI60475.1"
                     /translation="MFLTQSRENPERIRHVIESLPLRTDSTVLDIGAGPGTLSVPLAG
                     RVARVTAVEPAAGMAEVMEEYAAEEGVSNLSIVRKRWEDIDPAVDLDGPYDLVVASYS
                     LGMPDIKTAVEAMCDVSSGWVYLFWFAGTTSWEQAMVDLWPGLHGEEFRFGPKVDVLW
                     NVLYSMGICPNVETVRMEHTRRFSSIDTAVDEFREQYRITSPAQEAVLREYLAANLRR
                     ENGGGFLHSGTTTRVRLWWEANGCR"
     gene            complement(34573..35406)
                     /locus_tag="PLI76_00185"
     CDS             complement(34573..35406)
                     /locus_tag="PLI76_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI60476.1"
                     /translation="MHFRYGDFPVLSGVSFSVGKGELCGLFGPNGSGKTTLFKCCLRF
                     LRAEQGIVRMHGEDTSQCSIERLARLVAYVPQEHKPPFPYLVREVVLMGRTPHLGGFF
                     GVRRRDREIAMDALATLEISDLADRPYSQLSGGQRQMVLMARAIAQDTPVLFLDEPTS
                     ALDFQNQMRIWEIMRDIAGEGKTILACSHDPNHVAWFCDRVVVVGEGVVVAEGHPEDA
                     ISEVVLEEIYQNICSVRTLDGVQMVMPRCVVERNKKLKGDRPSHDVPLARAWSGAEAG
                     S"
     gene            complement(35430..36509)
                     /locus_tag="PLI76_00190"
     CDS             complement(35430..36509)
                     /locus_tag="PLI76_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOI60477.1"
                     /translation="MTESTGFNHLLKKLGYETVEGFGEERKILILLSLAAILFVTASI
                     AVVLGTYGITAADVYRTIFVHLTFGDVGSIPRLHNTIIWDIRVPRIILAVSVGGALAI
                     AGAVFQGVFRNPLVEPYILGVSSGAAFGAALGIVFPAIFFSIQVSAFAFGALAVTLAY
                     VLARSRGETPIITLILAGVIIGSIFSAFVSLLKYMADDTALREIVFWLMGGFYYTTWD
                     DVRLLVPVVILSFIILWALGWKLNVLSMGDDEARALGVSPEKYKFIVIALATGITAFA
                     VSLVGIIAWVGLMMPHASRMLLGPDNRFVIPASLMMGGCYLIVCDTLARTLTTAEIPV
                     GILTSILGAPYLCYLLRSKGRVIFG"
     gene            36661..37887
                     /locus_tag="PLI76_00195"
     CDS             36661..37887
                     /locus_tag="PLI76_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOI60478.1"
                     /translation="MTCNTKTIGLALLTACLLALAISCGCTGTTTDTGSVDSGARTVT
                     DMRGRAVELPATIERVVAIDDGFVEGLLYRFGMQDRIVGLGGTWVQDYTYPFEGAGGA
                     TYEYRNGTNPVSYLIPGLADLPVLVETGTAINYETLASLDPDVVILREGAWAFSAGSD
                     EALGKTIATIDSLGIPLVILVGPPFQESPTIGHIGEEIRLVGEVFSREKDADALASYL
                     DDQIKEIRRRTADIPENEKPRMMQLGLSPRARQGGGAGMAWGGDTIESYFIEEIANAK
                     NAYRGTGAFVVVSTEQILALDPDVIVLPTAQGYHPASELYTAPYYRNLQELTAVKNHR
                     VYALPWTPFNWAKRLEYPIEAMVIAKAAYPDRFADIQVHEWVLDFYQNVYGVDRATAE
                     ELRSAQWLDWMVEAEF"
     gene            37939..38421
                     /locus_tag="PLI76_00200"
     CDS             37939..38421
                     /locus_tag="PLI76_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2124 domain-containing protein"
                     /protein_id="HOI60479.1"
                     /translation="MELKEQLSGVPGMLRPFKAYLRGLEPGAGDQVVYYGCPGTCTPF
                     IELLGFAVRDLPVEQVYVPYVDETAAKIIRPVGGVGMQVSGDAARVDPEVVVLMGGLA
                     MPGVPVTKEAVRAVVGAHTGAKVVGICFMQMFEKAGWLDAFDFDLIIDAAIDPVRVWG
                     "
     gene            complement(38405..39832)
                     /locus_tag="PLI76_00205"
     CDS             complement(38405..39832)
                     /locus_tag="PLI76_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HOI60480.1"
                     /translation="MIALTKQQRVGVLITLLAISVFLTYYFHAVLMLGTVFSHFFYVP
                     IILTALWWEKRSVLVALFLGGLVVVSSLLYRPDILTLNDYGRALMFVLIALVVASLSG
                     QLKRREREVEKERDLVRHYLDVAGGLFVVINADHTIRLINRRGCELLGYCEEELLGKD
                     WFATVVPEAYQKARREIFDAAIASGTAYPDPRESPVVTRSGEELILAWQDTVLTGNDG
                     RPVGIIGSGSDITGRIRVEGELRAAHEEANLYLDIMVHDINNANAVALGYTDLLTDML
                     EGKEREMTLKLRGGVNRSIEIIQNVSTLRRLRSSETVTRPVDLDAVIRAGIAHHPDAG
                     IIYEGRPVEVMADDLLAEVFANLIGNAEKFGNPGVEVVIRVEESDGAVEVSVEDTGPG
                     VPDTVKPLLFTRFVRGTNRGSGKGLGLYITRMLISRYGGRIRVEDRVPGHPEEGAAFR
                     FTLPRPDRPWEPQPAVNPVAVTPRP"
     gene            40359..42590
                     /locus_tag="PLI76_00210"
     CDS             40359..42590
                     /locus_tag="PLI76_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI60481.1"
                     /translation="MGDVDIASFPPGVVEYLREFKTQEIRDIVETLLYIYIAQNASPP
                     RGEGTGVVEQSCYAYTSGPLFVLAEVGLIESSSRHATTLVRVTPAGEAIARPLAEARI
                     QALDLGAIARETHEIVPVLLAGTVRGSYVNKTFPSSLPRTEETFIGFLLNNNPALFEE
                     CERFAARLRAAGCAVLAHACDPDGAGTGSVVYAFPPEFAYILKRMLEAIDPGIREHYD
                     MLLESFHSTFTVLRYLACGEDYDRIRASAAHRRELSRMLSLIADSIYILDEVREDDTV
                     GLARFIVKDRDRYDAYLRDLARALMADVAQKIVIDRPAPAADPRPPVEEATPAPVRED
                     VIAALPPADDEREEEWDDAIFTDEDEEEPLAPPPPPAPRRIVAPRPAPEPPRPGGLDI
                     FLGHAQDGHRVDWSPGRLNNGHMIILGGSGAGKTETIRCIATELAAQAMPVVLIDFHG
                     DMAATTGDIRSYKIREGGEYYFNPLELDPAIDEITPLRATSDFVDAISINFPTLGIQQ
                     RRKIKNIIKDCYRASGITGNTGTWERVLDFDDIENEIMSCEDEAIPAYLEDIFDYKLF
                     SGEEKISIPTILSGGITHINLNALPENLRYLFADLFLRRIYYTLQATGEIPRGAKNER
                     EKFRLFVIVDEAKLLVSQKSGSKTAIKAVLNKYATEMRKFGVSLILASQLIAHFNEEI
                     LANIAVKFCMRAENKKQAQENAKFFEVSEKDLLNFQPGEGILIIGSEKMNVRIVPASG
                     RKV"
     gene            42630..43217
                     /locus_tag="PLI76_00215"
     CDS             42630..43217
                     /locus_tag="PLI76_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HOI60482.1"
                     /translation="MDALDDYRGKRDFSRTPEPPGEGTGARDRPIFVIQMHDATRLHY
                     DFRLEADGVLKSWAVPKGPSVNPKERRLAVPTEDHPIGYADFEGVIPAGSYGAGTVLI
                     WDRGTYKNLTQENGQEIGVTGAIGRGHASIWLEGEKLRGGYALTRFRTGKGEAWLLVK
                     MDDEEADPGRDPVATEPRSVASGRTLAEIAAGVGE"
     gene            43843..44289
                     /locus_tag="PLI76_00220"
     CDS             43843..44289
                     /locus_tag="PLI76_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HOI60483.1"
                     /translation="MASKVMTNSAVANSVMAEAVSDAEIVAQFRQRGCWGLYTSVDLK
                     GCDPASIRDAEKIHRFIIELCDLIDMKRFGEPQIIHFGPNERVAGFSMTQLIETSLVS
                     GHFANETNAAYLDIFSCKEYEPSKAAEFCKTFFGAETATYQVLFRD"
     gene            complement(44501..45388)
                     /gene="frhB"
                     /locus_tag="PLI76_00225"
     CDS             complement(44501..45388)
                     /gene="frhB"
                     /locus_tag="PLI76_00225"
                     /EC_number="1.12.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420 hydrogenase subunit beta"
                     /protein_id="HOI60484.1"
                     /translation="MDVLGNYKSVIAARSTDKDILKKAQDGGIITTLFAYALEEGIID
                     GAIVAGPGNEPWKPEPMVVSTKAELLAAAGTRYTISPNMSLIKEATRSYGLDRVGIVG
                     TPCQIQATRKAQLYPIGMRDVDDKIALALGIFCMENLSYQALEAIVEDHCNQKMESVR
                     KMDIGKGKFTVYTERGAVSQMPLKLIHKYVQPGCNVCLDYVANLADISSGSVGSPDGW
                     STVFVRSTKGNAVWDGAMKAGYFETKPMDQVKPGLDIVKKLATEKITKNQKNVDARKT
                     AGLKADGTPKGLRNPYVSP"
     gene            complement(45399..46160)
                     /gene="frhG"
                     /locus_tag="PLI76_00230"
     CDS             complement(45399..46160)
                     /gene="frhG"
                     /locus_tag="PLI76_00230"
                     /EC_number="1.12.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420 hydrogenase subunit gamma"
                     /protein_id="HOI60485.1"
                     /translation="MAEKITIGELHLSGCTGCLVTLADNYEGLFKLLDDYADLVYALT
                     LVDVRHVPEMDVCLVEGSCCLNDKISVEELKQARAKSKVLVAYGGCAAYGNITRFCRG
                     GQWNQPGQEAFVPISEVVDVDLYLPSCPPCPQAVRNVAVMAYLLLKGNDEQKKLATAY
                     LTPLMQLAQRGNEACGCDLMYDVINQGLCMGCGTCAGTCPVRAITMEYGRPNVNRDLC
                     IKCGACYAQCPRSWFNFDVMNNYEGIMDAIKGAMQ"
     gene            complement(46300..46860)
                     /gene="frhD"
                     /locus_tag="PLI76_00235"
     CDS             complement(46300..46860)
                     /gene="frhD"
                     /locus_tag="PLI76_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-reducing hydrogenase, FrhD
                     protein"
                     /protein_id="HOI60486.1"
                     /translation="MLFREIVIAGCGNPLYGDDGFGPAVVEELQKLQLPDNVKVIDAG
                     LGAPHFLFTLMEDAEVPVKKLIIIDIADFGADPGDVTKLRPEDLPPGAYRDAHSWDLS
                     EPLQRLKDVIDITIIGCQPKRVASHEFELGLTEEVEGAIPKTVRIVLEEIGVEYGAAI
                     NHQGTHLWASPGETSGEAGDKTGEQT"
     gene            complement(46871..48238)
                     /gene="frhA"
                     /locus_tag="PLI76_00240"
     CDS             complement(46871..48238)
                     /gene="frhA"
                     /locus_tag="PLI76_00240"
                     /EC_number="1.12.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420 hydrogenase subunit alpha"
                     /protein_id="HOI60487.1"
                     /translation="MSKVVEISPTTRHEGHSKLVLKVNDDGIVERGDWLSITPVRGVE
                     KLAIGKTMEQVPKIASRVCGICPIAHTLAGVEAMEASIGCEIPEDAKLLRYILQCANR
                     MHSHAIHNILSLPDMYIPGTDVKINPFTKEEPVRSVALRIQRLREIGQTIGEIVGGEA
                     IHPSNPRVGGMYKNITPRAKAKIYDLAKEGRVLAQEHMEFMVAVFKNFQNRDWSEVGG
                     VQVPIPDDLGYHNQGYMATAPVYGSSSLDENPTWFPERFTEVRPWDWYMGEVEVSEAD
                     PNYPIGGTSPVGTKAWPQMEACTGVPLYDGQPVEVGPRARLVQFRNYDGRGAMGLQIA
                     RQMEFPETAYGIIDALDALDTSGSVLADNIPQGDGSLGWAANEAPRGTDVHLARVKDG
                     RVQYFSMLVPTTWNFPTCSRALTGAPWRLAEVIMRAYDPCVSCATHMLVIDEDKRLVA
                     QKLIQ"
     gene            complement(48464..49405)
                     /gene="mptA"
                     /locus_tag="PLI76_00245"
     CDS             complement(48464..49405)
                     /gene="mptA"
                     /locus_tag="PLI76_00245"
                     /EC_number="3.5.4.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase MptA"
                     /protein_id="HOI60488.1"
                     /translation="MELPDVQSSLPEVRINLTRVGVKNVQKLVEVARPGKRPVIFISN
                     FDVFVDLPGSLKGANLSRNFEVIDDVLQQAIDGEVKVIEELCSAVARKLLDRHEYAER
                     TEVRMQSQFMVRRETPISETSCHEVVNVHASAIAQRNDGSPIIRKSIGAEVTGMTACP
                     CAQNIMKDHALHVLENLGVADDTINAFFEEVPMATHNQRGRGFLCIETDDDQHVSLEK
                     IIKILKDSMSARIYELLKRGDESYVVMEAHKNPRFVEDCVREMARKVITQFRDLPGDS
                     VVTIKQTNEESIHQHNAYAERKATIAELVGEMDEGAL"
     gene            49533..50522
                     /gene="ilvC"
                     /locus_tag="PLI76_00250"
     CDS             49533..50522
                     /gene="ilvC"
                     /locus_tag="PLI76_00250"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOI60489.1"
                     /translation="MVQKYYESDADPRTLEGRTIAVIGYGSQGRGQALNLRDSGCQVV
                     IGLRPGSSWQKASEDGFDVYSVADAVRRADVVQILLPDENQAAVYRAEIGPYLKENAC
                     LMFSHGFNIHYGQIVPPPTVDVIMVAPKGPGHMVRRTYEEGKGVPALIAIHQDASGKA
                     RAIALAYARGIGATRAVVLETTFAEETETDLFGEQAVLCGGVTSLIKAGFDTLVDAGY
                     APEMAYLEVLHEMKLIVDLIYEGGFTKMRDSISNTAQYGDLTRGPRVVGPETYAAMQE
                     ILEEIRNGEFAREWMLENMVNRPVFNALTRADEEHLIEQVGKEIRSLMPQFKK"
     gene            51074..51583
                     /locus_tag="PLI76_00255"
     CDS             51074..51583
                     /locus_tag="PLI76_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HOI60490.1"
                     /translation="MSVCIVFHSETGNTRTVAERLAPLVGGDLIEVTDLAHYSKISRY
                     ICGGRRAMKGESATIEPATIDVTACDTVVVGTPVWAGNPTPAINAAIEALVGIEGKST
                     VVFCTSGGMPGKTLETVTAMLAGRGADVRGAVPFTARDVQKGKGVEALADLILRAKRG
                     FDASAEPSR"
     gene            51711..52328
                     /locus_tag="PLI76_00260"
     CDS             51711..52328
                     /locus_tag="PLI76_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit beta"
                     /protein_id="HOI60491.1"
                     /translation="MTPSYEDLLKKAYTNITEPTEFEDRFTVPTARVFIEGKTTVLEN
                     FAEIAGILRRDQDHLMKHLLGELGTAGKVEGTRAVFSGKFEQEQINTIIRGYVEDYVI
                     CSECGKPDTRLVKSDRILMLRCDACGGHRPVRKRKAVADPGAAAKPTEGAIMDVTPQF
                     LSKRGDGVVKIDRYTMYVANAKPGQTVKVKITRIAGTIIFTERVD"
     gene            complement(52333..52944)
                     /locus_tag="PLI76_00265"
     CDS             complement(52333..52944)
                     /locus_tag="PLI76_00265"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOI60492.1"
                     /translation="MTKSAEGFARIAREVFAPVYPVIAEQVLAWSGIQSGLALDIGSG
                     PGFFAAALVEKSELSVIALDADPAMARIAQKTAADYRDRMIPVIGDVHCMPIRDGAVS
                     LIASRGSIYFWEDRPQAFREIERVLRPGGAAFVGGSFGTAALRNDIFREMRLRNPDWD
                     RDVARRSGGATPDLLRRELVASGVAHSRVREEEAGLWVEIRKT"
     gene            complement(52951..53235)
                     /locus_tag="PLI76_00270"
     CDS             complement(52951..53235)
                     /locus_tag="PLI76_00270"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit L"
                     /protein_id="HOI60493.1"
                     /translation="MAMKLKLLELTDDGARILFEGEGITYVNALAAELLNDPEVDVAQ
                     HRQVFRFADPELVVTTIGGRPPLLAITDAAKRLSGYAGELLRQMEALEAA"
     gene            complement(53381..54106)
                     /locus_tag="PLI76_00275"
     CDS             complement(53381..54106)
                     /locus_tag="PLI76_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOI60494.1"
                     /translation="MRITLLGTGDAIGTPKVGCDCETCRAMTAAGRSRLRTSFLIEIE
                     RKHILIESSPDLRQQLLRACSPHIDAVIWSHGHYDHFIGFGEFYRVQKVPPAYAAPPV
                     MDYCSRYLHFLPFEKHPVPVYEPFDLFGVTFTFFPVNHPPVYTCGLLIERDGITVAYT
                     SDTRKDIPEASRDLLLSRDIDLLFVDGIAPEGYNIDKHMNYAEAVRFARDVGARDYRC
                     VHMSHLVPPGMPHAGCDMETFCW"
     gene            complement(54108..55397)
                     /locus_tag="PLI76_00280"
     CDS             complement(54108..55397)
                     /locus_tag="PLI76_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI60495.1"
                     /translation="MTTETGTRDVLVVMELLLTAEIFNRNEALDINDLHPRYREFFGA
                     GSGGSTEVKRPLVVSEGAIKKTLGIPDAVCQAVRANPFVGYDEFGQRLSLPSLDAAAG
                     WFLKKSGVSRVGENPALACYFEGKDGITVSYRDVRAKIPRFEDTKEYIEAKVSRIIAG
                     SEEMREARDLLIISAPDEIEFTLDKLVCTPRQEEVIRKIAVALEHREFLKEHGIYEFG
                     RLLFVGPPGTGKTSLALAMSRALHMPVLEVRLAMVTSQYLGETSKNIDRIFDLAKRLS
                     PCILFIDEFDFVAKTRVSDDHGAMKRAVNMLLKNIDLISFVKNGVLLIGATNHPRILD
                     EAAWRRFDEVVEFPLPDQAMRQAIIEKVAATIDCNCDFADLAARTEGFSGSDIRMMMK
                     EAVISALMEDRRRLDPVDIEQGLFRVEERNVIRGTGY"
     gene            complement(55442..56323)
                     /gene="map"
                     /locus_tag="PLI76_00285"
     CDS             complement(55442..56323)
                     /gene="map"
                     /locus_tag="PLI76_00285"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II methionyl aminopeptidase"
                     /protein_id="HOI60496.1"
                     /translation="MIMDDVVYDAYREAGALARKVLHQGAGLVKEGAGLLEMVEATEA
                     MVMEEGALLAFPLNVSFNEAAAHDTAMPGDERVFSRGDLIKVDLGVLIDGYIADTAMT
                     VDLGDHAPLVEASAGALEAAIAAVRPGVAAGELGAIIQATIEERGYRPVANLTGHGLD
                     RYNLHARPTIPNVAMSGGAVIEEGMVFAIEPFATTGSGRISEAARVEIYRQIAARPAR
                     LPSAKRILEVARPRRGLPFARRWVPGDKVEIGLATLVRTGILHPFPVLHDIPDSFVSQ
                     AEHTLIVTADGCEVTTR"
     gene            complement(56320..57450)
                     /locus_tag="PLI76_00290"
     CDS             complement(56320..57450)
                     /locus_tag="PLI76_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOI60497.1"
                     /translation="MNGLDSVIRDAGAAAYAAYGSSADANVRYLTRFRTTDPVIYVAK
                     PGERGMIVVPQMEHERAVRESIAAVATRADAGYLEYIKAGESRWRATAHVIADLAGGP
                     VLVPATFPLALARELETFHPVVLDGAGSVEGMRAVKTPEEIERIRSVQRAAEAAIDRG
                     VTLIRSSAPRAGVLCRDGAPLTSEAVRTAMHTVLLAHGCRGVDTIVSCGPETALPHNT
                     GSGPLLENEPIVIDIFPRDELSGYYADMTRTVVKGEPSPEIREMYDAVRDAKTLAAAE
                     VRAGAAGADLYNAVVEFFRDRGYESDTRGFIHSLGHGVGLEVHEEPSLGPRGGALAVG
                     NVVTIEPGLYYPGIGGVRLEDMGAVTETDFDRFTQYEEELTL"
     gene            57507..57743
                     /locus_tag="PLI76_00295"
     CDS             57507..57743
                     /locus_tag="PLI76_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60498.1"
                     /translation="MMDEATKQAFKGRFVMLTVMLNIVILCFAMAAFVLLRFAPEGAP
                     GLVIGVILLAIGAAFSVSFWKRYTLTKAWLHEQP"
     gene            57777..58076
                     /locus_tag="PLI76_00300"
     CDS             57777..58076
                     /locus_tag="PLI76_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60499.1"
                     /translation="MLKKLKSEIELASRHLEVIRAVVEHQPIGIMKLSEILDLPYHRV
                     RYSLRILEQEGYIRASPAGAVATALAADLLSGLDAEIGELIGLLVTVQEENSRNL"
     gene            58116..58188
                     /locus_tag="PLI76_00305"
     tRNA            58116..58188
                     /locus_tag="PLI76_00305"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58149..58151,aa:Asn,seq:gtt)
     gene            59280..59447
                     /locus_tag="PLI76_00310"
     CDS             59280..59447
                     /locus_tag="PLI76_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60500.1"
                     /translation="MHRWVIDVPKLVLIDTETGMRVRHRIRSIKQAVKQQEWYEAKLG
                     RRIRIEKVFDP"
     gene            complement(59485..59931)
                     /locus_tag="PLI76_00315"
     CDS             complement(59485..59931)
                     /locus_tag="PLI76_00315"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HOI60501.1"
                     /translation="MLENLLDTAFQFKRVSSTLASSLFHQKEVMAVIARNIPGMGPWA
                     GEKSVFDALADETAYLHETAGNIREGLFSLIDARINAVSYEMNRAMRVIAVFSALVLI
                     PTLIGQTLGTNILGAPFTLYLWEVTAWTIISLFVVGWIFYRPGWLR"
BASE COUNT        11222 a        18844 c        18630 g        11292 t
ORIGIN      
        1 gatgccgata ccctgcgtgc ggtacgcccg ggtccgggcg ctgccttccc ggagcctctc
       61 cgacatgcct gtttcagccc tccgaaccct gcaccggcct ccctcgcggc aaacctcccg
      121 gccggtcgtt cacgcgcggg ataaaggccg gcatcccggc agcgggcggt gcatgataac
      181 ccgccctccc tccggcagcc tctgcccaga agcccccggc agaatcaccg cgatcgggta
      241 tgcgcgatat ccccggtgtc ggcggccggc gggagagccg gggagagaag gtgcgcgccg
      301 ggggcctgat atccacagtt ttttgagatt tcgccaaaac cttaattttg gttgagaatc
      361 cttgtggtgc gatcatcatc tcgatcgaga gcagggtcaa ccatgaacag gttactgata
      421 gtctcaaaca ggcttccgat cagcgtcacc cggaagaacg gcgacttccg cctgcaacgg
      481 agcgtcggtg gtcttgccac cggggtcggc tctttttaca aggcctacga gagcctctgg
      541 gtcggctggc ccggcatgaa cgttcagaga aagcaagaag aagagaaaga ccggatcgtc
      601 gatacgctca agagagagca gtgtcacccg gtcttcctct ccccgtacga catcaagcac
      661 tactacgacg ggttctgcaa caacaccctc tggccgctct ttcactactt caacctcttt
      721 gccgactacg accagaagac gtggaccgtc taccagaggg tgaacgagag gttctgcgat
      781 gccgtgatgg aggtcgtgcg gcccgacgac gtcatctggg tccacgacta ccacctgatg
      841 ctcctccccg agatgctccg ggagcgcctg cccgatgcgg agatcggtta cttccatcac
      901 atccccttcc cgtcgtttga ggtattccgg ggcctcccct ggaggcggga gatcctcgcc
      961 ggcctcctcg gggcggacct catcgggttt cacacctacg gctacgtccg ccacttcctc
     1021 tccagcgtcc gccgtatcct cggatacgag cactccttcg gagaggtcag gaccggcacc
     1081 cgggtggtgc ggaccgacct ctttccgatg ggcatcgatt accaccggtt cgccgactcc
     1141 gcgggcagca cgccggtcca gagggagatc gagagaatcc ggcagaagta cggaaagcgt
     1201 aagatcatcc tctcgtttga ccgtctcgac tatacgaaag ggatcccgct ccggctcgag
     1261 gcattcgata cgctccttga gaagaaaccg gagtaccagg ggaaggtctc tctcgtcctc
     1321 gtcgccgtcc cgtcaagggc cggcgtcagc cgctaccagg cgttgaagaa gcggatcgac
     1381 gaactcgtcg gtcggatcaa cgggaagtac gggacgaccg actgggtccc ggtcacctac
     1441 ttctacaact tcctcccgtt cgagacgctg gtggcgttct acagcgctgc cgacgtcgcc
     1501 ctggtgacac cgctccgcga cggcatgaac ctgatggcga aggagtacgt cgctacccga
     1561 accgacggta ccggggtgct catcctctcc gagatggcgg gagcggccga agaactcggt
     1621 gaagcgatca tcgtcaaccc taacgaccgg gacgcagtga tcgaggcgat cgaggcggcg
     1681 cttgccatgc cggagaagga acaggtcgaa cggaaccgga ccatgcagag acggctgatg
     1741 cgctacgaca tcgagcgctg ggtcggcgac ttcctcaccc gcctcggcga cgcccgggcg
     1801 atccgggtcg aacgctccga gcagatcgtc accccggcca tcagggagca gctggtcgac
     1861 gactatgccg ccgctcagga ccgcctcctc ctcctcgatt acgacggcac cctggtgccc
     1921 ttcgcggcaa aaccgcagaa ggcggtgccc ggcgagacca cgaaagaggt gctcgatgtt
     1981 ctctctcgtg tgccgggaaa cgaggtggtg gtgatcagcg gcagggaccg gtacaccctg
     2041 gatgcctggt tccgggggat ggatatcggg ctcatcgccg agcacggtgt ctggacaaag
     2101 gagcgttccg gggagtggcg gatgcacgaa gggctctcca gcgactggaa agaggagatc
     2161 tacccgctcc tcgaactcta caccgaccgc acgcccggcg cgttcataga agagaaggac
     2221 ttctccctcg tctggcacta ccggaggacc gaaccgatcc tcggcgcgca acgggcaaag
     2281 gacctcaaag acgacctcct gcacctgacg tcgaacctca acgtcggcgt catggagggc
     2341 aacaaggtca tcgagatcaa gaacaccgtc gtcgataaag ggagggcggc gttgaactgg
     2401 gtctcgcagc acgcctggga cttcatcctc gccatcgggg acgacaggac cgatgaagat
     2461 ctcttcgcgg cgatgccgcc cgatgcctac tcgatcaagg tcggactcgc cccgagcagg
     2521 gcacgtttca acctggtctc gcaacgggac gtcctgcccc tgctccggag gtgtatcgag
     2581 tgcggcaggg agggggtgac ccggaaaaga gagaaaccgg ggagggagaa gagtcttatc
     2641 gggtcgactg ctccggtatg acggatcgca ccgtcaggaa gaccggtccc ctgaggtcca
     2701 ccttcttgtt gtggtcggtg atataccgca tcgcgtactc ctcgatacgg aggttgacgt
     2761 cggaaggaag cttcgacatc ttcacctgga ctgtggttcc ctccccgcac cgtgcagcct
     2821 cctcgatccg ggcggcggca aggcctgcca cggcatcgag gtaggtgatg ccggtagaga
     2881 ccccttcgcg ccggacggat gcccacttct cggtatcagg gacaccgagg accgaaccgt
     2941 cgtgaacata gacctcgttt gcgcacgcgg gcccgcagag tttcgtgttg gactcgggct
     3001 cctcgacgat cacctcgacc tctctccccg caatctcgcc cttccacgca gtgaacgcgc
     3061 acgggcccgg ggcggttgcg tgctcagcgg ccgtagcacg gatcgcctcc gccatccgct
     3121 tcccggtcgt cgacgccggt tccgtgcgga gatggacggc gcccgcgata tcgcggtcgg
     3181 agagggtctg cgggaagaac tgcgggtagg tgagctcgcg gatatcgtta gaccggtagg
     3241 cgatcatggc gagccgctcc accccgagac cgaggttcat caccggcacg ccgatcccgt
     3301 actccgcgag cgccgagggc gagtagatgc cgaacgtggc gacctcgacc cagtcgagga
     3361 ccgggtggcg ggcgtagacc tcggtctgtg tcccgggcat gtagtacttc gaccgcttct
     3421 cgtcgggctg gaacctgaac tcggtgaacc cgaaggccga gaggagggcc tcgctgacgg
     3481 ctttcccctc ttcgagggtg acgtcctccc ccgcgacgac gcaggaggcc gagtggtagg
     3541 ccatcaggcg ggtcggcccc tcctcctgct cccgccggaa acagcggtcc accgagaaga
     3601 gccggatagg cagatgtcgc ttctcccaga tggccgagag cgtcaggaac cagccgctcg
     3661 tcatgtggct ccggagggtg ttgcgcgacg actcgggggc gagcgcgcgg aactcgggga
     3721 agaccgcgtc gaggatatgc accacggcgg catcgtcggc ctcgaggacg gccgcaagct
     3781 cgtgcgtcag gtcgtcgccg tcgatcgtcc ccttcttgta gccgtgaagg gtttcgcgga
     3841 gtctctcctc gaccgggggc gtgatcgtgc ggccgaggat cgcctcgatc ccctcgatct
     3901 gctttcttgc aatcccgaca ttcgggcggg gcaggccgcc gaggtagaag acccggtcga
     3961 ggacggccat cgcttcgggc ccgaactgcc ggtagatatc cgcttcctcg acgatcaggg
     4021 ggttcatcgc ctcgtcaaac cccatcgaga ggtaggtctc gcggagcctc tgcgtgatct
     4081 caaatatcgg gtgcgccgtg gcccgggtgt acttcagacg gggatattgg cccgagatcc
     4141 ccggcggggt cagcaccgac ggcccttcgt gccaggcgtg ctcgaagtcc tctctcgctc
     4201 tctttttgaa ctcttccaca tcgaatctca tatcttccgc tccaggcaga cgacccggct
     4261 tgccgggctc tcgtccttta cggtatagtc gcagtgttct gcaatcttct cggcaaacgc
     4321 ccgcacatcg ctatgttcgg gcatctggtc tctctgcaat cgttttctac tgtatcccag
     4381 gtacatataa cccttcactt cgacgaatcg ggcgccggag tcctggtaga tcgccgcata
     4441 cccttcaggc gcgaagtcgt tgtagccgcg caccacggtg gtccggatag cggagcggcg
     4501 gccggcaagc ccggcaaggc tctcctggat gcagtcccag tagtcttccc gcggccggca
     4561 gaggccgagg taggtctccc ggtcgggagc atcgagagag acgtagacct ggtagggccg
     4621 gcaccgcgcg aggacgtcgg gccgggtgcc gttcgagacg aggaacgtcg tgtacccgcc
     4681 ggcgttgagg ccgtcgatga ggtttgggag ttgcgagtag caggtcggtt cccccgagag
     4741 ggataccgcg accatcgtgg ggcggagggc ctccgcgaac cgttccggcg cgacgtaagg
     4801 cgagaccttg tagcccgcga gcgccttctt ctggaggcgc gggaggctct ctatgatcgc
     4861 ctccggcggg cactcctcct cctccacgac ctcgtgctcg aacgaccgcc agcagaagag
     4921 gcactgctgg ttgcagcgta gcgtcggcgt catctggacg cagcggtggc tctcgatgcc
     4981 gtagaactgt gccttgtagc acatagctcc gccccggagt gcccgcttgt tccacatgca
     5041 gggttttacg gccgccgacg aggcagggct gacgaactga tagccctgtt tccggagggc
     5101 cttacatgct tctgagtgca tcgataccga gtgtcgcaag cgactcctga agggtgttcc
     5161 tgaccgcatc cacgagggca aggcggctgt tccgggcctc gccctcactc ttcagcaccg
     5221 ggtcgtagtg gtagaaggcg ttgaagaggt cggcgagctc gcgggcatac gaggcgagca
     5281 ggtgcgggcg aagctccgtc acggtctgct cgacgacgac ggggaaccgg gcaaggtgcc
     5341 gggcaagcgc gacctcgtgc tccgtctcgt agtggtatgc cggcatcgag accgctcctg
     5401 ccttatcgag tatgctgcag gcgcgggcat gggcgtactg gatgtaaggg ccgctctgcc
     5461 gctcgaagtt caacgcttcc cgccagtcaa agaccgtgct cttctcgggg gagaccttca
     5521 cgatatcgta gcgaatggcg gcgacggcga ccgatcccgc gatggcccgc cgctcccctt
     5581 cggggagctc cggccggcgg acggtcacct cgtcgtatgc cttcgcggtc acctcctcga
     5641 tcagttcgtc cgccgagacg aacttgcccg cccgggtgct catcgagccc tcggggaggg
     5701 agacgaactc gaagtagacg atatcgggcg gctgttcgcc gagaagctcg agggtggccc
     5761 ggagctgggc gccgatcagt ttgtggtccg ctccgagaat atcgatcatc cggtcgaagt
     5821 tgcgcgcctt ccaggtgtgg taggcaaggt cgcgcgtgct gtagaccgac gtgccgtcgc
     5881 tcctccggag gatgtacttc ttctcgaaac cctgctccga gaggtcgacc cagaggatct
     5941 cgtcctcgtg ggcctgcggc aggcgcctga cccgctcgat gatccggtcc acatcgccta
     6001 tccggacgaa atcgctctcc cagacgaacc ggtcgtggcg ggcgttcagg gcggcgaggg
     6061 tcgctttgat gccttccgcg cagagggaga cggccgcccg gaacttcctg acggtctctg
     6121 catcgccgcg ctccacctgc tgcatgaggc ggtcgatctc ctccgtgatc tccgggttct
     6181 cccggatcgc ccggttcgcc gcgacgtaga cccgggcgac gtaatggtcc gccttctcac
     6241 cctccccgcg ggcaaggccg aggtggtcga atccccagga gacgatggcg atctggcggc
     6301 ccatatcgtt taagtagtac tgcgcctcga gcgggtatcc ggccttccgg aacgcccgga
     6361 ccagggtatc cccgatgacg gtgttcctga tgtgaccgac gtggagcgga ccgttcgggt
     6421 ttgcgctcgt gtgctcgagc accacccgtt cggtgcggcg ggggaggttc ccgtaaccgg
     6481 gttttgtcgc cgccctgaca gcctccgaga gaagcgaggg gccgaaccgg aagttgacgt
     6541 aggggcccac cgcctcgacg cggatatccc cgagatcggg gtccgccgag agccttgccg
     6601 cgatatcggc agcgatcttc tggggtgccc gcctctcctt cttcgcaagt ttaaacgcta
     6661 cggtcgaggc gatatcggcg tgatcccccc ctatcgtgag gaggacgtcc tcttcgccgg
     6721 tgcatgcccg gagcatgcgc tcaacctggt ggtacttctc ctgaaacatc agtatccggt
     6781 cctataacga agtattgccg cgatcccgcc gaatgcgttg tagagcatcg agccttcctc
     6841 gaagtcgtcc gatatgatcc gaaccgtcgt gctgctctgg tccgcaagcg ccgtcagctc
     6901 gtcgatgatg tcgacctcct ccgcgacgta gagagaggca ccgtcattcg ggcaggtgcc
     6961 gaagtcgatg tccttgatat gtttgcccgg ttcgatgctg atcgttctct cttcggtgta
     7021 gtcgctgttc gggcaggcaa gtttcagccg ggccctccgg agcttatcgg agaggaggag
     7081 ggtatcgacg gagccgagtt ccaggttctt tcggacgctc tcctcgccgt aggcggccgg
     7141 cccgtcgtct ttgaccagtt cctggaggaa ccggttcatg atgttcttct ccttgatgat
     7201 atcgaggccc ttcagcgcat ccgatgcgtt ctcgacgagc tcggcaaggc cggactcgtt
     7261 tgtgtaggcc acatcgaaga gcccgatgat ccgcttctgg agctcgtggt gaaggtagcc
     7321 gcccgcctcg aactcctcct tcgtgggggt cgggccgccg atcaggacgc ctttgaaccg
     7381 ctcgaagaag tccttctcgg cgaggaagat atcgtttgca aggtcgccga ccttcttgta
     7441 gaactcgttg atggcgatca accgcaaccg ctggaaccgg acgctcgact gaccgccctt
     7501 ccgctgcttg ccggggaccg tcgatgtggc gccgcgcacc gcctcgatcc ggttgccccg
     7561 gaggaaaccg atgtaggcct cccgccggtc gatgacgatc aggccgtaga cctccttctc
     7621 gccgagcatc tgctgcaggg gctcgagctc gaacgaggaa ctgcaccggt acaggtaggt
     7681 gttgagcggt tcaggcggct cgatgatctc gcactcaagg tcggtgcggt cgccgccgat
     7741 gttgaccgtg ccgcagaaga ccgccatgcc gttctccggc gggcgcttgt agtacttcag
     7801 gcgggagagg atggaggata tggcgctctg gacgtttgtc cgggtctgtt tgctcttgat
     7861 gttcgcacac tgtccgaact cgtcgcggag ctgggcggtc acgtcgtata tctgcttatc
     7921 cggaggtata tagagcgtga tcagctcggt gccgctcccc tccttctcgg caagcctctc
     7981 aagcatcttt ttaaattcgt agcgctttcg cgcggtatcc atctcgtggg tctcttccat
     8041 cgggtattca ccgggctcgc tgaatggtat tcactggtcg aagctactat gtgtttctcc
     8101 ggagatggct taaatctgcg cacatttgcc atccccctcc cgcgcccgcc ccgggccggg
     8161 cgcctcgcgc tcttctcaca agaagcaccg gcagatgaga cagatcgtct ttgcgtcgac
     8221 tattcgccca tcggcgatca tggccgcaag atccctgagc ggggtgcgga cgacctcgat
     8281 cacctcgtcc tcgtccatat cgtagtcatg gcagggcgag aggccttccg cgatgtagag
     8341 ccatatccgc tcgtcggtgt aaccgggcga agggtagatc cagcctttcg gggtgaagac
     8401 ctccgccttc atcccggtct cctcgatcag ttcccggcgg gcggtctcgt gcggctcctc
     8461 gccctcgtcg atggtgcccg ccggcgcttc gaagatgtaa tcgtcgacgg cgtagcggta
     8521 ctgccggatg aggtaacagt cgtctccctc gaccgggagg atcgcgaccg ccccgccggg
     8581 atggatgacc acccgctcct ttcgctttcc attcgggagg gtgatctctc tcttctcgac
     8641 ggtgagtcgt ttcccgcggt agatctccat ggtcagtcct cgtacgcgat cggatcgcaa
     8701 agtccgagtt cgcggaatgc agctcgccgg tagtggcacg aggagcagac cccgcacgcc
     8761 cggtcgtcgt tctggtagca cgaccaggtc tcctcgtagg ggacaccgag gcgaagaccc
     8821 acgcggagga tatccgcctt cgtcatacgg acgaagggcg tcatcagggt gatcggggga
     8881 tccgccgccg tgccgagatc gatcgcgtgc tggaacgcct cgatgaactc cggccggcag
     8941 tccgggtacc cggggtagtc gccggtctgg acgccgatga agatcgcctc cgcccgccgg
     9001 gcctccgcga ggctggtcgc gatggcaagg aggtttgcgt ttcggaaggg aacgtaggtt
     9061 gccggcaccc ccttctcggt atactcgtcg atcgggatgc tctcatccgt caggctcgag
     9121 gatccgatct ttttgaagtg ctcgaggccg atctcgacga actccgccgc atcaagcata
     9181 cgggcgatgg tccgggcgca tgcacgctcc cgcttctccg tcctctggcc ataggcgaag
     9241 tggagcgcga tgatgtcgta gcccatatcc tttgcggcgt aggcgagcgt ggcagagtcc
     9301 ataccgcccg aaagaaggca gacagctctc atcatttcat ccccaggatc ttgtggagct
     9361 gtagttgaaa cctcaccggg aggttctctt cgacgatata atccgcgatg gcacggtagt
     9421 cggatccttc caccggtgtt atgatgacct cgccccggat atcgtaacgg gccatcaccg
     9481 ctcgcgcgta gaggaagtcg ttctcgtccg ccaccacgaa cttgacgcag tcgcgccggc
     9541 cgatgaaggg gaggagagag agatcgcttt gctcccccga tgaggggcat ttcacgtcca
     9601 tgcagatgga ggcaaacgcc tgcatatccc ggaagtcctg agttccgttc gtctcgatct
     9661 cgaccgtata accgtggcgg tcgaactttt cgaggagccc ggcgacctcg tcccgctgca
     9721 ggagcggttc cccgccggtg atgcagatct gcttcccgtt caggagccag acccggtcga
     9781 ggacctccgc aacgctcatc ctcatcccgc cttcccacgc gtaggaggtg tcgcaccagg
     9841 cgcaccggag gttgcacccg gcgagccgga tgaacgtgca gggccgcccc tggttcctgc
     9901 cctctccctg gaggctccgg aagatctcac tgacgatcat acgtgcgctc tgcgtaacag
     9961 gacgtcgact cccagacacg gatcttcttc acgcgggcat caagcccctg ccgcgcggcc
    10021 tcggcatcga tcatatccgc gatgaggccg gcgaggagtt cgctcgtggg gtcgccgggc
    10081 gtggtgacga ccgggtggaa cgcctcgatg cacgccgcca tggggtcgcc gacgttcagg
    10141 atcacctggt ggtcgaaccg cccgacgacc tcctttatgc agttgtagtc aaggacgatc
    10201 ccggtgcgct cgtccgccgt cccctcgatc cagacctcca cccgccactg gtgaccgtgc
    10261 aggcgaaagc acttcccccg atagtggatc aggcgatggg tcgcctcgaa aaaaacctct
    10321 ttgtatatcc gggttgtcat ccatgaaggt tgctcgccag gatataatat tatatcagct
    10381 cccgatcaaa taactagggc gaaagatcga cgcgcggatg ggttccacaa tatataaatc
    10441 cgtaacgcgc gttgctttat agcacaccgg gagttgctcc tatccacaaa ttcaaccgat
    10501 acgagggtat tcaatgggaa atggtaaatt cgcagcccga aaactgaagc gcgacgcgaa
    10561 aagagacagg tggcatgatc ctgtctacgc ccggcgccag cttggacttg atgtgaaatc
    10621 cgaccccctt gagggagcgc cgcaggcacg cgggatcgtt cttgagaagg tgggtgtcga
    10681 ggcaaaacag cctaactcgg cgatccgtaa gtgcgtccgc gtacagctga tcaagaacgg
    10741 ccgccaggtg accgcgttcg ccgtcggcga cggcgcgatc aacttcatcg acgagcacga
    10801 cgaggtcgag atcgagggta tcggcggcag gctgggcagg tcgatgggcg atatcccggg
    10861 cgtccggttc gtcgtgacca aggtcaacaa cgtcagtctc agcgagatgg tcatcgggcg
    10921 caaggagaag ccgcggaggt aaggagtatg gaagaagcag cagaggcagg catccaggac
    10981 cagaaactcc tcttcaaccg ctgggacatg agcgaggtgg agatcaaaga tccgagcctt
    11041 gcccgttacg tgaacctgca cgcgatgatc gtgccgcact cctgcggcaa gctcgccggc
    11101 cagcagttca acaagagcaa tatgctgatc gtcgagcgcc tgatcaacaa gatgatgcag
    11161 accgagcaca acaccggcaa gaaggagctc gccatccgca tcgtcaagga tgccttcgag
    11221 atcgtcaata aaaagacaaa gaagaacccg gtccaggtgc tggtggatgc cgtcgagaac
    11281 gccggccccc gcgaagagac cgtccggctg aagtacggcg gtatcaacgt cccgaagtcg
    11341 gtcgacaccg cgccccagcg ccgtgtcgac accgctctcc ggttcatcac cgacggtgtc
    11401 cggcaggcga gccacaagaa gaagaagacc gtgagcgagg cgctcgccga ggaactgatc
    11461 gcgaccgcaa acggcgacac ccgctcctac gccgtctcga agagagaaga gagagagcgt
    11521 atcgcaaagg ctgcccggta acatcccctt tacttttttg gtgttctatg accagacgaa
    11581 agaagatggt agagcgggtg acggacctga tggacaagcc ggaacacatc cggaacatcg
    11641 gtatcgtcgc ccacatcgat cacggaaaga caaccctctc agacaacctg ctcgcaggag
    11701 caggcatgat cagcgaggag ctcgccggca ggcagctctt catggactct gacgaagagg
    11761 aacaggcacg cggcatcacc atcgacgcaa gcaacgtctc gatggtccac gagtacgccg
    11821 gcgaggagta cctgatcaac atgatcgata cccccggcca cgtggacttc ggcggcgacg
    11881 tgacccgcgc catgcgcgcg gtggacggcg ccgtcgtcgt cgtggatgcg gtcgaaggca
    11941 ccatgcccca gacggagacg gtgctccgcc aggccttgaa ggaaggcgtc cgcccggtgc
    12001 tcttcatcaa caaggtggac cgcctgatca acgagctcaa ggtggacgag caggagatgc
    12061 agatccgcct tgcacgggtg atcgataagg tcaacaagct gatcaagggc atgaacgaga
    12121 agatgtacaa cgagggctgg aagctcgatg ccgcgaaagg caccgtcgcc ttcggctccg
    12181 cgctctacaa ctgggcagtc tccgtcccct acatgaagaa gagcggcgtc tcgttcagag
    12241 atgtctacga gaagtgcaac accggcgaca tgaagtggct ggcaaagtca agccccctgc
    12301 acgccgtgct cctcgatatg gtggtcaagc acctcccgaa ccccctccag gcccaggacc
    12361 ggcgtatcca catcatctgg cacggcgact acacgagctc cgagggcaag gcgatgctca
    12421 cctgcgatcc gaacggtccc gtctgcatga tggtcaccga tatcaccttc gacccgcacg
    12481 caggcgaggt cgccaccggc cgcctcttct cggggacgct ccgccggggt accgagtgct
    12541 acatcatggg tgcggcgaaa cgcgcaaacc gcctcgccca ggtcggcatc ttcatgggcg
    12601 ccgagcggat cgaggtggaa gaactggccg ccggcaacat cgccgccgtc acgggcttga
    12661 aggatgccat cgtcggctcg accgtcacga cgctcgtcga tatgaccccc ttcgagtcgc
    12721 tgaaacacta ctccgagccg gtcatgaccg tcgccgtcga ggcgaagaac atgaaggatc
    12781 tcccgaagct cgtcgaggtc ctccgccagg ttgcaaagga agacccgacg gtgcaggtct
    12841 cgatcaacga ggagaccggc gagcacctga tcagcggcat gggcgagctc cacctcgaga
    12901 tcatcaccgg ccgtatcaag cgcgacaagg gcgtcgatat catcacctcc ccgccgatcg
    12961 tcgtctaccg cgagacgatc acgggcaggg cgggcccggt cgaagggaag tcgccgaacc
    13021 ggcacaaccg gttctacctt gaactcgaac cgatgaaccc cgaggtcgtg aagctgatcc
    13081 agcagggcga cgtctcgatg acccagcagg cggtggagcg gcgcgacgcc ctcatcgccg
    13141 ccggtatgga caaggacgag gccaagaacg tcaaggcgat cgagggcacc aacatgttca
    13201 tcgacatgac gaagggtatc cagtacctca acgagacgat ggaactggtc ctcgagggct
    13261 ggcgtgaggc gctccacgga ggtccgctcg ccgacgagct ggtccagaac ctgaagatcc
    13321 gcctggtgga tgtgaaactc cacgaggatg ccatccaccg cggtcccgcc caggtcatcc
    13381 cggcggtcag gagcgccgtc aaggcgggcc tgctgatggg cggcgattcg ctcctcgagc
    13441 ctatccagaa gctccagatc acggtcccga gcgatcagat gggtgcggcg acctcgcaga
    13501 tccagggccg gcgcggccag gtcttcgata tgctgagcga aggcgacacg atgacgatcg
    13561 tcggcagggc gccggtcgcc gagctcttcg ggttcgcggg cgacgtccgg tcggccaccg
    13621 aaggccgcgc gatgtggagt accgagttcg ccgggttcga actcgtcccc tccggcatcg
    13681 tggacgacgt ggtcaagggc atccggcgga ggaagggcct gaaggagcag ctcccccggc
    13741 cggaagacta tctcgcgtaa ctctcaatcg aataccctcc aatttttttg cggcccgggt
    13801 cccgggaacg gaccgcctcc cgccccgttc atcttctgcg ggccccccgg cgcttctgcc
    13861 ggcgcgccgc agatctccgg tgacagcccg accatccgct tccgcttccc accccgaccg
    13921 aatgattgat gtggggagga tgtatctcac ggtgggggga tgtcatgacg accgcagatc
    13981 cgggtatgca ggctgtccgg gcttcagcgc ccgggcgagg gcgcatcacc gcctgttccc
    14041 tcccggcggt catccccgtg ccgctcggcc cgaccacagc gttcatcgtc agggatcggg
    14101 gggtgctcct gatcgatacc ggtaatcccg gggatgaagc ggccatcctg gccgcgatga
    14161 ggcgtgcggg catccgcccc gatgatctct cgctgatcct ggtcacccac ggccacccgg
    14221 accattacgg gagcgctgat gccctccggg accggaccgg cgcgccaatc gccgtccatg
    14281 cggccgacgc cggcgccgtg cgctgcgggg ttgccggacg gttctctctc gcccggatcg
    14341 tcccgcacca gatcgggttt atggcgggga agacaggaag catcgagccc gacatcgtca
    14401 tcgagggcgc cgccgatctc gcggtcttcg ggatccgggg cagggtcatc cccacgccgg
    14461 gccatacccc gggctcggtc tcggtcttca tcgccggagg cgccgccatc gtcggcgatc
    14521 tcctctccgc cctcctcccg ggcgggaggc cccgtctgcc cttccgtacc ggcgatcctg
    14581 cggcagcgcg acggagtatc cgggcccttc tcgccctcgg accggaacgg atctacgcgg
    14641 cgcacggggg gccgtgggcg ggggcggagg tctcgcgatg gttcgggagc gagtggtgac
    14701 gacgggcacc cggggggatg accggatggc ggcagggcag agatcgggat gtcaaaaaag
    14761 gggatgccgc tcccccgcac ggttgcggaa gagggcaggg gggatcactc gatggtgaac
    14821 gtcatcgaga acgtggtctc gcttccggtc acgttcatcc agggctgcat atagatcgcg
    14881 gagaactcag cggatccctt ctcggcagcg cggtactgcc actcgtgcac gcccccggca
    14941 cccacaaggt ccgtctttgg cgggatgaat gtgtcgttca cgtacgagag cccccgggac
    15001 gaggtgacgt tccatgaata tccggtcgtc gggttctcgt cgaggctgat gacgatctcg
    15061 ctcccggccg ggagggtgac ggccttgttg tcgtcggtct cgttgaaggc gaaccgttcc
    15121 gccggagagg gtgtgaccgc cgtggttgcc gccggagagg gtgtgaccgt cgtgttctcc
    15181 ggttgcgacg tgcaaccggc catgagcatg caggctacca ggagtcctgc aatcacgata
    15241 ccatggattc tctttacctg aatcatgaat gagtgtccgt atatcggcaa taaaaacttt
    15301 cctaaaccat tttgcctatc gcgaggagcc tgtacaggcg tcggaagaga cggatcgcgg
    15361 atcagagaag accggccgac aaaccggaac cgtgttccgg cccggggaga gatgctgttt
    15421 tcgatgaatg acaacgtata aatatatcca tcccgtctat catcctctga gggcacgata
    15481 atgaaaacca cctcctcgaa actgctcttc ccggcgctca tcgtggtgct cgggatctgt
    15541 ctctgcatga cggcgggctg cacatcgagt tcttcggata cgaccacgcc ggaggcagcc
    15601 acgacggctc ctgctgccgg caccaccgtc tccggtgccg ccagaggaag cgtaaccgca
    15661 gttccgtttg aaaccctgat aacgttcttg ccggccgcac ctgccggctg gacggcagaa
    15721 gagcctgtgg gcatcacgat ggcggtcgaa gacggccagt ggacctggtc atcccgggat
    15781 tacacaaagg gcgacgccca ggcagcaatc gtgatccagg actccgccta ctacaacgtg
    15841 ggccaatggc agatgtggaa cacgttcacc tcgatcgaga cgactgaagg ctactacaag
    15901 tcaaagacaa tcggtggtca ccccgcctgg gaagtcttca gcaagcctga ctcctatggt
    15961 acctgggtcg gtgtgaacga acggtttatg gtctacgtca gcgtagacgg cggctccaaa
    16021 caagacctcg atgcgttcgt gaacgccatc aactacggcg gcatagcaaa cctgaagtaa
    16081 cccaaaaacc cttttttacc ggacccggcc gggccccggc acggccgtga tgatgtagtg
    16141 gtagggcccc gagtccgcca ccgccgcgac cgtaagcccg gcctttgcca tcaatgacgc
    16201 ggcatgctcc gtggtgaact tctttgcgac cggcgggccg aacgggaggg gttccttctt
    16261 ccagtccaga tcgacgatca cgcccccggg cgccgccatc ctctttgcgt tcaagagtac
    16321 ccggaccgga tcggtgaagt cgtggagatc gatgccgaag aagacgacgt cggcgcagcc
    16381 ctcgcagagg acgatctcct cggccatttc ccgatggagg gcgatgttct caagcccttc
    16441 accgcgggct ctcttcttga gggtgtcgag cgccccggca tcgatatcga tcccgcagac
    16501 acgaccgcgc ggcccgacta tccgggctgc aggaagcgcg aagaacccat ctccgcaccc
    16561 gacgtcgatg aaggtggcgc cagcggcaag cccgatcccc tcaaggatgg actcggggtg
    16621 ctgccagcgc ctccgtgcag cctcatccac catcgagtgc cttcccgatc gcataaagtg
    16681 tatcgatctc ggtagcatat agcagaccag agcctgctgc tcctcaaaac aacctccggt
    16741 gaacaaccga cgaaaagggg tccagggatg cccccttcac ttcatcgcct tccggaccct
    16801 ctcctgcact tccgggtgct cgagattcat ctggtgggcg gtctggacgt gcccctcgat
    16861 ccgcttcatc agctcgttct cgccctccgc cttctcttca aattcacacg caaggccgag
    16921 atccttgcac ctgaacgtcc tgacgttctg catacctctc accggccgga ccggtataac
    16981 gcgagtaata taaaaatatt gtcgccgcat tttcttcctg caaccccgct gacgcagcct
    17041 ttgcagtcgg atgcattcgc ttcgcgaaca ccgcatcgag gcgatgtgcg ggggcgctcg
    17101 tatcaggccg tccggcccgg gggcgggccc tccctcctgc actcctccgt atccccgatc
    17161 agcccgtccc gcagggtgac gacccggcag aagtactcct tgtgccactc ctcgtgcgag
    17221 accatcacga tcgtctggtc gaggtcctcg tttaaccgcg cgaaaaggtc gaggatgctc
    17281 tttgacgcct ggctgtcgag gttcgcacag ggttcgtcgg caaggaggat gcttgggttg
    17341 ttcacgagcg cccgggcgat cgccacccgc tgctgctccc cgcccgagag ctcgctctgc
    17401 cgatggctcg tccggccggc aagacccacc cgctcgagta tcgcggtgct cttcctgagg
    17461 tactcctcct ccggatcccc cctgacgagc gatgttaagt agacgttctc aagcgccgtg
    17521 agctccccga tgagggcgta gtcctggaag acgtagccaa gccggttcaa ccggaagagg
    17581 gttcgccggt ggtcggagag ggcaaggaca tcggttccgt cgatcgtgac cctgccggag
    17641 gtgggacgat cgaggagccc gaggatatgg aggagggtcg atttgccgct gccgctcgct
    17701 cccatgatac cgacgaactc gcctttccca acctcaagcg agacgccgcg gagggcctgg
    17761 acctccaccc tgcccatggc gtagacccgg gtgagattct ctgcgacgat catggttcac
    17821 cccctgatag cggtgaggat atcctcgttg gcaacccgcc aggccgggat ataaccggca
    17881 gccaccgaga ccgcaaagag gcttgcgatg ctctggatga cggccgacgc ctccacggcg
    17941 ggggagaccg gtcctccggg gaaggcgagc gggtaggcag tcaggtagat cgtcaaaccg
    18001 gcagtgagag ctatcccggc cagtgtcccg acggcggtga tgaagagcac ctggaggatg
    18061 taggagttga cgatgacccc ctggctgatg ccgatcgcct tcagtatacc gatctgccgc
    18121 cgccggttca cggtgttgat gaagatcacg atgaagacga cgacgacggc gatgatcagg
    18181 ctgacgagtg ccgatatagc ggtgatgatg tcgaaactct ggatcgcctg gttgacaaac
    18241 ccccccgact tctcctggta ggtccagata tcttcctgga taccgtagga gaggagttct
    18301 gtcttgtatt ccgcctccct gccgttgaca tcggtcttca ccagaatgat ggaagcctga
    18361 tcgtggagtc ccagcactcc gttcatctcc cgggcggtga cgaaggcaga cgcatcgatg
    18421 ctgatggatt gcgtctcgta gatccctttc acgcggtagg ttcgcgcttc tccgttcggg
    18481 tagaccacct ctaccggact gccgaccacg accccccgga gggaggggag gccgcccgcc
    18541 tcctccccct cccgcccggc aagggtcgtg cccatgatga tcgcgtcggt gtccccgttt
    18601 gagagaaact cgccctcgac catacggtcg gcgatcttcg tcaccgcccg ctcatccgca
    18661 ggctttatac cgtagagggt gctggcggcg ttgcggcccc ggtagaagta ggtcaccccg
    18721 gcggctatcc ggggcgatgt gccggtgatc cccgggatcc gatcgacctt cctctgaagg
    18781 ttggccgcgt cgtcgatgta ctggtcccct tcccggggtt cgatgaccag gttcccgaag
    18841 tcgaagtcga tcgactggtc atcgaaggcc ctgacaaccc cggatatgat ggaggggagg
    18901 aagatcaggt tcacgaagat gagggcgatg atcatgatcg tgagggcgag cgtccccttg
    18961 ctcccgcgtt ctatggattt ccaggcaagg aacgcggaga ccctgagacc ggtgaacatg
    19021 gggaaggtca ctcctccttt cgtctccggt accagtagag cgcggctgcg atgacgacga
    19081 ctatggcgac caccgcgatg atcagggccg gggcccggtc ggatccggca acaaccaggt
    19141 tcagggcctg ctcggtcgtg tgcgctccgt aatcgtcggt atactggatg gcgagggtat
    19201 aggggtaatc cccctcctcg ccggcctgca ggttgaatac ggcgggaccg tcgttgccgg
    19261 gctctatcgt cccgaggaac gcttcttttg ttcccgaaag cgagaggccg ccgatggtcg
    19321 cccgaaccga cgtcgcatcc gccgttccgg tgttctctat ccggatcgtc aggcctaccg
    19381 gatccccggc ggtgatccgg gcaggttcgg tcctgatgga ggcgacgctc atatcggcgc
    19441 ggccgacgat ggggataccg atcgtctcga cctgccggaa caccgcgaga tcggcgctcc
    19501 ggtagtcgac ggtggcgacg accggcagaa ggccgagcgg cgcgccggga tcggtcccaa
    19561 aggataggtt cagggctgcc tcctctcccg gcctgagtac cgggatatag taactctccg
    19621 ggctcgtcga ggcgatcgag tcggagcttg cgttgatgcc gaccgatata tcgttcgcac
    19681 tcgcctcccc gtcgttatag agcgtgagcg tgacgccgaa tgagtcgccc gggtcgacgg
    19741 atgccgggac ggtcctctcc acccggagcg agggtttttt cgccagggcg taggcagaac
    19801 cgacattcac cggtatgggg tagcgcacgc ccgtcgcatc ccgcacgctg acccagacct
    19861 ccgggaaata gatgccttcc tccccgggag cgcgtacctg aaacgtgatc gggagggatt
    19921 ggcccggacc gatctcgccg acgtcctgga aactcccgga caggacctcg atctctctgc
    19981 tgcgtaagag tacgttctcg atgtaggcgt tatctggggt gatcggggag gtgttcaccc
    20041 ctcctgtatc cacccggagg tcgagcggcg accccggggc catggatgcc gtgctcgtga
    20101 gcaccaccgt gatcgtccct tcgtcgccgg gcatcaggac ggcgggggtg acggtgtagt
    20161 tggagacgat caccgtcggg gcacctgccg ccgctgccgg gatgagcgcc aggagtatga
    20221 tgaacatacc ggatatccag ccaatcgtgc gcctccccat gctcctgccg ggagcagtcg
    20281 cgggctcctg aatcatatcc ccctccccgg tgcggttctt ctggacccca gagtccccgg
    20341 tgcctgtata tatctgttgt gcacgggttg ctctccgccg ggcccggggc gataccgcga
    20401 cattgtgaag gtcattccac ccagaaatat atttatggcc ccatgtgtat actacacccg
    20461 ggatacgggg gagatagtac cggatgggtt gatcgggcag ttcgcgacac gtatcttcac
    20521 ccatgtttga acgctctata actctttaca gaacgtcacg gttcggatac cctcaggccg
    20581 tcgtactacc tctccgttct catctcacgg ttctccatcc tttgctccat accnnnnnnn
    20641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntgctggc aggaagtctt ccggtcgcaa
    20761 gctggcttta tattccccga tggtatactc cgcccacgtt acgaggggaa agagatggag
    20821 acaacccagt ggctggtgac gctgacgttc agggtgacgg tgggcggtgt ccggtcgaag
    20881 gatgattcgg tggctgccgg ccttgagcag ctgaaacagt ccctgaacag cggggaggat
    20941 gtcccgatgg aggcaaccgt caggaggatc acaaacatcg tcgaggagga accgatcttc
    21001 ggcgtgaggt ggtgaccggc gacgccgtga ttttgcggcg gccatctccc ggagaaaacc
    21061 tttttttacc actgccgcct gatccggccc catgcacgaa gttcccgata cgcttgccat
    21121 cgacgtcgtc ctcctgccgc ccgggccgat catggagatg gcgatcggcg cgaaccggac
    21181 cctgcttgcc ggcaacccgg gcggcggtat acgccttgac ggcgaggact gcctcccgca
    21241 cgtctcggtc gcgatgctcc cggcaaggcg cgaggatatg ccggagatca tcgcgaagat
    21301 ggaccgaatt gtccgccggt gctcccccat gaccatgacc atagatgccg ttgcgaaaca
    21361 ccgctcaagc acgggggaga ccgtctccgc cttccacatc ctgcgggcgg agatcctcgc
    21421 gctctttcac aagaccgtga tgaacgtcct ggcgccgtat gcgacgcttc cggccgggcc
    21481 cccgatcttc tttgaagaag cgtctgcgcc atccatcgac tgcttcctcc ggttcccgga
    21541 gacatccgcc tacgagcgct actccccgca catcacgctc gggtttggcg acctccccga
    21601 actgctcccg ggaatcgatc ttcccgtcag gttcgaggtg acgaaggccg ccatctgcca
    21661 cctgggcagc cactgcacct gccggcggat catcgccggg ttcgatctcg ggaccggcgc
    21721 ctccgctgcc cacgggaggc cgatccggtg agcgatcggc cggttaccgc agcagcaccc
    21781 tgacgtattc ccctgtacgg atggatggac ccggaacgat ttctctgccc gaaaaccttc
    21841 ttagccccga cgtccccagt atctgcatgg atctgctcac ggcccgggcg acgttccggc
    21901 gtccccttct ccctgccgtt cccggtacgc cggcggcgat cggcgagact ccgggcgggc
    21961 ggtgcgcatg aagctgacgg tcctcgtgga taacgccacg ctcaccgacc gctactttct
    22021 cgcagagccc gggctctcga tctatcttga agacggcgca acacgtatcc tcttcgatgc
    22081 cggttactcg ggcatcgtcg tctccaacgc aaggaggatg gggatcgatc tcctccgcgt
    22141 cggcgagatc gtcctctcgc acggccatct cgaccacacc tggggtctcg atgcgctgct
    22201 ccgtctccac accgaggcca tcatcgaggg gcgcgagcgg atcgaaccca cgttcatcgc
    22261 ccatcccgac gcattcctca cccggagctt cgggggtgcg ggagagatcg ggtgccacct
    22321 ctcggtcgag gaactcttcc ggcacgggaa ggtcctcctc acggcgtccc ccctctggct
    22381 gaccgagaac ctggtcttcc ttggcgagat caagcgccgg ttcgatttcg aacagatgcc
    22441 ctcgatcggt tacatctaca cccccgacgg tatccgggac gataccgtcg ccgacgatac
    22501 ggcgctcgcc tgcaggactc cggaagggct cgtcatcata accgggtgcg ctcacgcggg
    22561 gatctgctcg atcgtcgagc aggcaagaga ggtctgcgac gaggaccgga tcgccgacat
    22621 catcggcgga ttccacctcc tcaacccctc gccggggagg atgcagggga tactcgacca
    22681 cctcgccgag gtgcggccgt cggtcctcca cccctgccac tgcacggatc ttgcagcgaa
    22741 gatcgcgctt gcaaaggtcg cggacgtccg cgatgtgggc gtcgggctgc acctggaata
    22801 cggctgatca ggatgtgtct ttccggagcg ccgggatctc cccgtgctcc gcctcgtaag
    22861 ccctgacagc ctccggagcg ccgagaagga agcggcgggc gttgttgaag atggccgggt
    22921 gctcaccggg atcgagggag gcaaggaggc ctacgagcac gcctgccacc ggcacccgat
    22981 ggcggggctc catcgcatcg atgaacgcga acgcatcgag agcccgggcg gtgctgtact
    23041 cgttcacccg ggcgagacgc cggaggacct ccccaaggta tgcccgtccc tcctccgtct
    23101 caagcgaccg tttgcggttc aacgcaaacc gcccgtaaag cgcccgctgc tcgcctgcct
    23161 gctcgaccga gaaggcgggg gaggattcga tcctgcggag gatctccacg atgtcgtcgc
    23221 agtcgctccc ggcgacgacg gcgaggaagg actcccacgc caccgggttc ttcgccgatg
    23281 cagcacagaa ctctgcaagg atattcaggc gttccggggc gctgctctcg aggatcagcc
    23341 cgaatgcggt gaggcggtcg gttgcggccg cggcttcacg gaactgcccg gcgagcaggc
    23401 gatgaatctc cggggcatcg cgggcggcaa ggattgcaag gcacgtattc ttcctctgcc
    23461 gccgccgtat cgctctggct tcgtcttcga ggtagccgtc cccgctcggg agatccaccg
    23521 ccgcctgccg gtagaccgcg aggagggtat ccgcgtgccg tgcgccgaca gcggcaagga
    23581 tcttcttcct cgcatcgtgg agggcgcggt aacggtgggc gaaccgctcg tcgtccaccg
    23641 ccgggaagat ggtcaggaac tgaccgcccg cctcctccat gagggcatcg tcggcgagga
    23701 ggccggcaca gaggtcggcg aaccggctcg ataccgccgc gcccggatcc tcgaggaggc
    23761 ggagcatctc gtggccggca agagcattga acgcggtgaa acggccgacg atatcggcat
    23821 ccttcgctgc ctggaggtag agttcgtcct ccggcggccg gtagaccacc ttcccgtaga
    23881 aggagtagcc gcggttgagc gagaggaacg cgggccggtc gacgtcgtcg atgacgacct
    23941 cctcctcttc gcctgcgatg cggacggttc gctctgcgat atcccgaccg tcggcatctg
    24001 cgagagcgaa ccggaacggg aactcccggg ggcggccgcc gggcgagcgg ttctggcggc
    24061 aggcaagggt gaaccgccgg gccgcgggat cgtaggaggg ggccacggtg aggaccggat
    24121 actccttctc tttgagccag accgccgcca tcccggtgaa gtcctgcccc gagacctcct
    24181 ccatgcagcg gatccagtcg tccgttgagg catttttatg ccggaaccgg tcgtggtaga
    24241 gggcaagccc cactgcaaac gtctcctttc ccatcagcgt ctcgatcatc ctgacgaact
    24301 cgggagcttt cacgtaggtg acgcccgtga tgaggtcgtt tgggtcgtca aagccgtccg
    24361 gctcgatcgg catcgagccc gcgccccggt cgagggcgag cgtgccggca acggggtcga
    24421 ggaggtcgag cacggtctgg aggcgggagt aggcctcgcc gaagaagaac gcgtggtgcc
    24481 ggttctcgat atggaccgtc accgcctcgt tcaaccagat ctcgaacgga ctcctccccg
    24541 tcacttcaga gccgttctcg ttatggtaat actcgtggac tttcaccctg accatgtact
    24601 cgaaggccgg atcggtcgcc tccgggtagg gcatgatccg gtttgccgcg atcgtcgtgt
    24661 tcccgacgtt ctccatgccg ccgaaatcgg agttctgcat cgcgatctcc cggtagaccg
    24721 agcccgtgta ggcgtagccc ggggtgacgg ttgcaacgag ccctgcaagc gcttctctcg
    24781 tcctcacgag agactcccgg tccccggcgc gtttagccgc atcccgcacc cggatgagtc
    24841 gccagatctc ctgccgggtc tttcggtcgc ggtacggttc cgggccggtg aagaggtgga
    24901 cccacatcac cgcatcggca agtatatcga gcgcacgctc tggcggctca cccggggcaa
    24961 aaccgggttt tgcgaggatc tcgagggaga acgcccggcc gtcggggtac tcgaactccc
    25021 gccggtgggt atcgtagcac cccacgccga ggaagaagag gtagggggcc atcggggtcg
    25081 tggtgttccg gtattcctgg acggatcggc cgggcgcaac gaggtagcgc ggtcccgcgg
    25141 ggtcgccgtt cgacagtatg ttcgtatacc cgtcgtccgc ggcgatcgtt gtcgtgtagg
    25201 tgcactttgc ggtcatgtcg tcgaggcagg ggaccagccg ctggaacccc cactgctgac
    25261 actgggtgat ctgaacgggc ggccctcccg ggcaggaccc atcgtaatag aggccttcga
    25321 gggtgtggct gctcgggcgg cagatcgtct cggtatcgag ggtgaaccgg gtccgcggcg
    25381 gaacgggagg gtcgaaggtg acggtgagga tcgcagcacc gcggtcgtaa gcgtgggcga
    25441 tgctgtgccc gtcgcaggca accctgagta tatccaggtc gcgagcgttc agggcgaggg
    25501 atgcgatcgg tgcgtcgagg gtctcggcgg tgagggccga gacgacccgc gtatgatcga
    25561 cgtagacgtc gaaggcgagg ttcatgtgga cgaccttcac ggtgagcacc ccgaaatctt
    25621 ccggatagta gcgaaaaagc ctcatacccg gtgtcagaat tccatctcgc cggtgatatc
    25681 gcggagtgca cgacggatgg ccgtccggac ctccggttcc tcctcgcact cgagcgcggc
    25741 ggtaaggggg ccgactgccc gggcatcgtg gagtttcccg agcgcatcgg cgacggcaat
    25801 cctgacatcc ttcctgctat ccgtaaggag gagaatcagg ggttcgaccg cgcaggggtc
    25861 gccgatctcc ccgagagacc aggcagtgcc gcgccgcacc caccggtcgg gatcggcgag
    25921 gagggggagg agaggttcga ccgcccgtgc atcgcagagt ttcccgagcg cctgcgccgc
    25981 gacccagcga acctcgttct ccgggtcgtg cagggcggcg atcagcggtt cgaccgcccg
    26041 ggtgtcggcg caccctccga gcgcctcggc agcccgcaac cggtagggcg tgctctcatc
    26101 gcagagctgc tcgatgtatt tgcctatgtt ctcctcgcgc cgcttctccc tgtcttccat
    26161 gaggcgtttt accgagcgtt ccatcttaat ccctccgcct gtttctatcg gaaattaatg
    26221 tcgttatggt atattattct gcccggtatc cttatcccgc ttcttctcca tccccaccga
    26281 tgacgtcgaa ggagacgatc ctgtaatggt aataccgccc atccggggcg gtgaacgaga
    26341 ggaagaccat cagcccgtat tcctccgata tccagagctc ggcatcgtcg tcgatggtgt
    26401 agtagccgaa cccgaacccg gagagggcct cctcgatggc gctgtcggcc tccatctcct
    26461 ggaccatccg aaacgtcgtg gcaagggcgt actgccgatc ctcgagggcg gatacctgga
    26521 gccttgcgcg cttctctgcg agaaagaggg tgtccagggt ggccatgatc tcctgcgcga
    26581 cggtatccgt ctcctccgac cccatacccg gtactttggg cgtgccggta tatcagcgta
    26641 tcaccgcgcg gggatctccg atccacgccg tgaacaaccg cctgttcgta aaaagaccgg
    26701 ggttgcacat ccatcccatt cccgagcccc ggcagcgaag ctctaaacca tccggatgcc
    26761 aatacaggtt tgatggcaga agagagactc aagatcgtta tattcggctc gttcaacgcc
    26821 ggcaaatcca gtctcatcca ggcgctcgat ccccggagcc ggcatgtcga ggcagcctcg
    26881 gacgaaggcc caacgaccgt cgccttcgac ttcggcaggc tgcaggtgag ggaccgggcg
    26941 gtctacctct tcgggacgcc ggggcaggag cggttcgagt tcgtccgtca gatcctctcg
    27001 cgagggatgg acggcgcgat catcgtcgtg gatgcgacca ccggggtgga tgcgatgacg
    27061 cggcatcttt acaatcagtt gaaggacctc ggggtaccgc tcgcgttcat ggcgaacaag
    27121 tgcgaccgtc cggacgcggt gcccgaggcc gtccgccggg aacttccggg ggagatggtg
    27181 caccccatat cggcacggaa cggcgagaac gtccacgcgg cactcgacgc cttcgtcgca
    27241 accctggtcg cccggtaacg atcattttta ctgtctgcgg gtcattgagc tcttgagatc
    27301 attgaccgcg aaagagacct acatcaggga tgttgcccgc ctgctctcgg gctttatcgc
    27361 gagcgggaac acaacggatc ttcttggcta cctgaccgcg gagggcggcc tgcccggggc
    27421 agatccggaa ctcaccgggg cgttcaccgg ggtcgtacgg gagtttgccg ctgccgaccc
    27481 cgacgatcga cgcctcctct gggacctctg catcatgctc gccgacatcc ccccggaagg
    27541 tgctccggca agcgacctgc acgaactcct tatctcctgc ggtatcaggg gcatcgggac
    27601 gatcggctcc ctctccccgg cgtgcgcgga accggcgctc acccgccttg cggaggcgtc
    27661 cgccgatccc cgacagcgga tccgggaggc ggcagggatg ggggtcaggg atatcctgtc
    27721 ccggcagcgg gataccgtga ttccagagct cgcacgatgg gtggaaggag gctcctggct
    27781 caccatgcgc gccgcaatca tggggatcgt cgagccggac ctgctcggtg agcccgatat
    27841 cccgacggcc gccttccacc tccatcgaaa ggtcctgatc cgtatctaca cggcgaagga
    27901 acggcaatct gaggcgttcg gggcactcag ggcgacactc ggctccacgc tcgccctggt
    27961 catcgccgcc ctgcccggga taggattcga atatctccgg caactcgcaa ccctcgatga
    28021 tccggatatt cggtggatcg tgagggagaa cctcagggaa agcgggctcc agaagcgtta
    28081 ccccgagacg gtgcggcaca tcggggcgca gatagggtaa agaagatgcg aggccgccac
    28141 taccccaccg ggcgggttta cccgcgctgc cgatgggaag aggcagggat ggccgggcga
    28201 ccgatgcccg gcgccgccgc tatgttgaac gccgcgttta cgtcggcatg gtcggtgtag
    28261 ccacaacgcg ggcaggtgaa ccgcttcccc ctgcggatac cctgttcccc gcaccggctg
    28321 cagcaccgcg acgtgagcgc cgggtcaacg agggcgatca cgacaccggc gtcgtgcgcc
    28381 cggaccatga ggaggttctg gaggtggaaa aacgatccgt cgcgccggga gaacagaagc
    28441 ggagcgcccg gtttttgcca ccccgcaaat cccgcgccgg tgagatcctc gagtttgatg
    28501 ccgcactcga ggtttcgagc catcttcacg atctctcttg caatcctccg gttcagatcc
    28561 ccccgggcgc tgctctcccg gtcccgggcc gccctgagcg ccttacgttt ccccccgctg
    28621 caccgctccg gggtgccggc accctccggc tgctcgccga gcatcaccac gcctccgctc
    28681 gccggatggg ccacgacggc gatagagccg gtggcgttca ggtcgacacc gatccagtcg
    28741 ccgggcggcg ggctacctct gataaacggc atcgtccccg gggtcgcagg tcttcggtct
    28801 catgcgcttc tggtgtatgg acaggaatgg tcccccggcc accatatacc caccggaccc
    28861 gccatctcaa tcgctatagc gcctccaggc ctcctccacg tgcagcgcct gcatgccaaa
    28921 cttccggggc gccgcgacgt cgttctcggg attatccccg atgaagagga cttccggagc
    28981 ggaaagcctc atccgcccga gcgccgcggc gtatatccgg gtatccggtt tctggtagcc
    29041 gagatctgaa gagaagatga caaagtcgaa gcgatcatag agcccgaacg cccgcatctc
    29101 gcactccgag aagacccgct gcccgttcga gacgacaccg agcttctccg cgcagaagag
    29161 gtcaagcagc ctccgactct cctcgatgat gccgagacgc cggagcgatg cggcccggaa
    29221 cgcccgcgcg gtctcgacac cgagccgccc ggcatcgatc ttccggatgg catgttcggc
    29281 gcagatgccg gcaaagatct cctcgacccg gacctcgggg tagggctccc cgatctgttc
    29341 gatcgcctcc ctgacatggc aggtgtagag aacccgcagg tcatccggag cgaccatgac
    29401 cccctggtag atcagccatc gcgcgaggtt tcggtaggtc tcggtatcgc gttcatcggt
    29461 caggatatcg atgagcgttc cgtagcagtc gaagagaacc cctgctaccg gtggatcgca
    29521 cagagacacg cctccgcctc ctctaaaagc cagtcacgtt ccgctgcccg ccagggcaac
    29581 cgtgcaatcc ggaggtatcc gagagccatg aagaagggaa ggacggcggt atagcgccgg
    29641 aactcttctt cacccctgct ataatgccag agcaggtggc cgatgtagtc ttcggccgtc
    29701 gagatgctcg cgcccgacgt tcttagctct gcagccagga tgccgagatc gcgtatcggg
    29761 tgggtgtggt tccgggatcc ttcaaagtcg atagcacagg tatctccgtt gaagaggtag
    29821 ttcgtgagcg ttgcatcgcc atggaccatg catccctccg ggccgaggcg cgcactgtgc
    29881 caccacgctc cgagcaggcg gtcgaaccgc ttcctccggg aaccggaaag gttgttctga
    29941 tcgaggatcg catggtagta ggcaaaatcg cgctcccgct tgcaggaagt ccgtgtgctg
    30001 tcgtggagtc ggcggagcag gtgcgcgaca ccggtcaggg ggtcgtacag ggatacgcct
    30061 gcaccgagga gttctcgcac ggtcgggccg gggacatgct cggtcacgag gacggaatgg
    30121 aaccggttgt tcgtggcgat ggcatgggga acgcggatcc agcccgatac acgccggagc
    30181 cttaaaaact cgttcttcat cgctgcatcc gtgttgtagc gggttctcgc tccggtcggt
    30241 gcaccgaaaa atttaccgac gacgcttggc ccgccggcga actcgtaccg gcagacgaca
    30301 tgggatgcag gttctatccg gtagacccgg atctttccac gccacccggt caatcgatcg
    30361 gcaaggatgc ccgcgagcca gtcacggaac gcatcgccct cttccagacg tcccaccctc
    30421 ctctcggtgc ccacccggca tcctcctcat cccggcgcaa ccccaccggc agggcccgga
    30481 aggcgtttca ggactatgaa gttaccggag agagggatcg acccacacct ttttacgttc
    30541 tccgcgcctt aacggggtgc atcgacccct ccggaggagt gatgagccat gatgccgttt
    30601 tatgagttcc ttgaggagat cagacctgcc gtcaaccgac ggatcgggga ggtgacggga
    30661 gatgagggtt cgatcgaccc cggcgtcgtc ccgctccttc tcaggggtaa gaggatgcgg
    30721 gccgggctcc tcctccatac ccacgcggcc ctcacgggca gaaaggcgcc gacccgccgg
    30781 gcgctcgacc ttgcctgtgc cgtcgaactc gcccacgccg caagcctcat tctcgacgat
    30841 atgctggacg gcgacatcct ccggcggggg ctgccctccc accacctcac ccgggggacg
    30901 gggcgggcgg tcctcgatgc cgtcggggtc ctcgccctcc cgtacgcgct cgccgccccc
    30961 tgcggtgccg aatacgtggc aatgctcgcg tcggcccagc agagcatggc ccggggcgcg
    31021 gcctgggaga tgctcggggg atccgggctt tcgtcgatcg agctctatga tgcgatcatc
    31081 gcaagaaaga cgggatgcct cttcgcgctc gcagccgcct ggggtgccat ggcggcgggc
    31141 gaggacgagg atgtcgtcgc tgcgtttgca gacttcggcc tctttaccgg gaaggcgatg
    31201 cagatcgccg acgacatcgc cgacctgcac gcgcccgcgg cgggggtccc gggtcgtcgg
    31261 cccgggtccg aggtgctcct cctccagggc atcgccggcg aggacaggac cgatcaggcg
    31321 ctcgcggcga ggctcgatac ggagatcgcc cgggcggcag accgactcgg ggacggagcc
    31381 cggcagcggt tgacgcccgg agagcgggag gccttcgggc aggcggtccg ggatatcgtg
    31441 gggctcacga tcgcgggggg tttgccgccc ggcaccgcca cgtttcaggt cgtccgcccc
    31501 ggcgcccttg tgtaccggtg aacccctccc cgcccttacg gatggggcct ccggttcata
    31561 ccgcacaccg ataaataccg tcccatccca catttttcat catgccggtt gtaaccattc
    31621 gtatggcaaa ggggcggacg cttgagcaga aacgaagact tgccgccgag atcaccgcgg
    31681 cgatcacgga gacgctcggc gtcgatccgg aacgtgtcac cctcttcttt gaggaactcg
    31741 acaccgagaa catagcacgg gcgggcaggc tccttgcgga atcgtaacgg cgcggcgggc
    31801 gaacgttctt gtagccgccg ctcccgggag tgaacgaaac catggcacgc aaattcgacg
    31861 agagagattt tgcaatcctc cggaagctgg ccccggaata cagcgggatc ctctgccctg
    31921 agtcgggaca cgagttccac tcgatactgc cgccggtctc caaccacatc gcagcggacg
    31981 aggaagactt tgcggagcgg gtcggccgcc tctcggatga ggactggata tacctcaccg
    32041 accggatcct ttccggccgc gaagacctcg ggtgcatgcc cgaggaggac atcgagacca
    32101 tcctcatccg gatcgagaag gcggtatcgg atgaggcggc tgaacggatc cgcaggctct
    32161 accgcctctc cgggtgcgga gtgctctgat cggccgtcaa ttcaccctgc cggcggctgc
    32221 ccccgcatcg cgagcgagcc ttcccgaccg gatgggccgg aagatcgcga tatcccgtcc
    32281 agcacatccg caagaataac gatcgcggga tggttcacgg caagcgcccg gccatcgggc
    32341 ccgcagcaag ggctgtgggg tcctccgtga tctcgcaggt ggcccgtgca gggccgggcg
    32401 agcgtgggga atcgattccg ttcagcatcc tgacagcctg taccggattg gtcgtcgtcc
    32461 cctcacccgc acgcaggtca cacctccacg caccagtcca tccactgcgc cgatcgcagc
    32521 gctttcgctg tcccgtcatc gacaccatag acgttcttgt agaagtcaag gagccactcc
    32581 ccgagatcga tatcggcaaa tcgtccggga tacgctgcct tcgcgatcac catcacgtcg
    32641 atcgggtact cgaggcggtt actgtagtag ggtgtccagg gaagggacat gacccggtgg
    32701 ttcttgacgg ccgagagttc gccgacgttc ttgtagtaca tggcatcgta gagttctcca
    32761 ggcggatggt agccgtagga cgtgctgagg acgatggcgt cggggtcgag ggcaagaagt
    32821 tgttcggccg atatgagggg gcttcccgcg ttccggtaag cattctttgc gtggacaatc
    32881 ttctcgatga agtacgactc gaccgtatcg gttcccttca cgctgccagc cccctgggtt
    32941 ccgagcgccc cggccctgtg agcgttcatg ccgaatacaa ggacggtcgg ccgttcggca
    33001 tcaggaatat cctttgttct ctcaaagacc acctgtgtct gcgattcaag gtaatcggcg
    33061 agtttatcag cccgttcctc cttcccaaag acggagccta tgatccggat ctcatcggag
    33121 atcgtcgcaa tatcaggctc atcgaagcat ggaggacctt tgagtacgac gatgggaatc
    33181 ctgagggact cgattgtctg gatggtcttc tgcacccctt cattctccat cgaggtgagc
    33241 cctccggtac cgacgcggag gatgacgaca tccggatcga gtccggcaag ggtctcatag
    33301 ttaatggccg catctgcccc catgatcagc gggagttctt tgatcggcgg gtagagaacc
    33361 tgcaggacat tgcgtccatc actcccgttc acgaactttc catcatcaag gggataggag
    33421 tacacccaga cgttctggat gtttatcccc ccaaccccga cggtcttctc ctcttccccg
    33481 aggacgaaca tcacctcctc gatgaggccg ctggagacgg tgacgacacg gtcgatctct
    33541 ttcggcacct ggacctcaac cccgcgggag tcgacaaccg tccggtaacc ggcggtgtca
    33601 gcgcccgaat ccggtatgcc tccgctctga ccggcaccga cgcaccccgt aacaaagacc
    33661 aggaaagcga ccaggataag gatcagggct gcctgtcccg gtatccccct caccggcatc
    33721 cgttcgcctc ccaccagagc ctcacccggg tcgtcgttcc tgagtgcagg aaaccgcccc
    33781 cgttctcccg gcggaggttc gcagcgagat actcccgaag aaccgcctcc tgcgccggag
    33841 acgttatcct gtactgctcc cggaactcgt ccacggcggt atcgatactt gagaacctgc
    33901 gggtatgttc catccgcacc gtctccacgt tcgggcagat gcccatcgag tagaggacgt
    33961 tccagaggac gtcgactttt gggccgaacc ggaactcttc cccgtgcagc cccggccaga
    34021 ggtcgaccat cgcctgctcc caggacgtcg tcccggcaaa ccagaagagg tagacccatc
    34081 ccgatgacac atcgcacatc gcctcgactg cggttttgat atccggcatt ccgagggagt
    34141 aggaagcgac gacgagatcg taggggccgt cgagatctac cgccgggtcg atatcctccc
    34201 agcgcttccg cacgatcgag aggtttgata cgccctcttc ggctgcgtac tcttccatga
    34261 cctccgccat acccgccgcc ggctcgacgg cggtcacgcg ggcaacgcgg ccggccaggg
    34321 ggaccgaaag cgttcccggc ccggcaccga tatccagcac cgtcgagtcg gtcctgagcg
    34381 ggaggctctc gatcacgtgg cggattcgtt cggggttctc gcgcgactgg gtgagaaaca
    34441 ttcgagcctt ctcccgggag gcccataccg aggcgcatcc cccgttcccc cgagtttccg
    34501 tatttttttc gtagaggtct ttccagacgc cattccagtc aatctctcta tacatcgttg
    34561 ttcatctccg tttcacgatc cggcttccgc cccactccat gccctggcca gagggacgtc
    34621 atgagaaggg cggtcacctt tcagcttttt gttccgctcc accacgcacc tgggcatcac
    34681 catctggacg ccatcgagcg tccggacgga gcagatgttc tggtaaatct cttcaagcac
    34741 cacttcggat atcgcgtcct cggggtgacc ttcggccacg acgaccccct ctcccacgac
    34801 cacgacccgg tcacagaacc acgccacgtg gttcgggtca tggctgcagg cgaggattgt
    34861 ctttccttct ccggcaatat cgcgcatgat ctcccatatc cgcatctggt tctggaagtc
    34921 aagcgcactc gtgggttcat cgaggaagag aacgggagta tcctgcgcga tcgcgcgggc
    34981 catgagcacc atctgccgtt ggcccccgga gagctggctg tagggcctat ccgcgaggtc
    35041 gctgatctcg agggtggcaa gtgcatccat cgcgatctcc cgatcgcgcc tccggacgcc
    35101 gaagaatccg ccgagatgcg gagtcctgcc catgaggacg acctcgcgga caaggtaggg
    35161 gaacggtggt ttgtgctcct gcggcacgta agcgacgagt cgcgcaagac gttcgatgct
    35221 gcattgcgag gtatcttcgc cgtgcatccg gacaataccc tgttctgcgc ggaggaaccg
    35281 caggcagcat ttgaagagcg ttgtttttcc cgagccgttc gggccgaaga ggccgcagag
    35341 ttcccctttc ccgaccgaga aggagacgcc cgagagtaca gggaaatctc cgtatcgaaa
    35401 atgcatatcc tggacgtgga gcatggcagt cacccaaaga tcaccctgcc cttactgcgg
    35461 agcaggtagc agagatatgg cgcaccgagg atggaggtca ggatcccgac cgggatctcg
    35521 gccgtcgtca gcgtccgggc gagagtgtcg cagacgatca ggtagcatcc gcccatcatc
    35581 agggacgccg gaatgacgaa gcgattgtcg ggcccaagga gcatcctcga tgcgtggggc
    35641 atcatgagcc cgacccacgc gatgatcccg acgagcgaga cggcgaatgc ggttatgccg
    35701 gtggcaagcg cgatgacgat gaacttgtac ttctcagggc tgaccccgag tgcccgcgcc
    35761 tcgtcgtcgc ccatcgagag gacgttgagc ttccacccga gcgcccagag gatgatgaaa
    35821 ctgagaatga ccacggggac gaggagtctg acgtcatccc aggtcgtgta gtagaatccc
    35881 cccatcagcc agaagacgat ctcgcgcaac gccgtgtcgt cggccatgta cttgaggagc
    35941 gagacgaatg ccgagaagat cgaacctatg atgactccgg cgaggatgag ggtgatgata
    36001 ggcgtctcac ccctgcttcg ggcgaggacg taagcaaggg tgacggcgag tgcaccgaac
    36061 gcgaatgcag agacctggat cgagaaaaat atagccggaa agacgattcc gagcgccgca
    36121 ccgaacgcgg cccccgacga gacaccgagg atgtaaggct cgacgagggg gtttcggaag
    36181 accccctgga agacggcacc cgcgatggca agcgccccgc cgaccgagac ggcgaggatg
    36241 atccggggca cgcggatgtc ccatatgatg gtgttgtgca gcctcggaat acttccaaca
    36301 tcgccgaacg tgagatggac aaagatcgtc cggtagacat ccgcggcggt gatgccgtac
    36361 gtgcccagga cgaccgcaat agaggcagtt acgaagagga tagcagcaag ggagaggagg
    36421 atgagaatct ttctctcttc gccgaacccc tcgacagtct cgtaacccag ttttttgagg
    36481 aggtggttga agcctgtgga ctcggtcatg cgatctccac ccacacgggc ggtgcgggta
    36541 caccctctct ctgtattaac cattatataa attgtagcaa ttaatacgac tttgacatat
    36601 agttctcttc attgatgtga aatgcgatcg tttttatagc atttatccca actatcccct
    36661 atgacttgta atacaaaaac catagggctc gccctgctta ccgcctgcct cctcgccctg
    36721 gccatctcct gcggctgcac gggaacaaca acggatacgg gatctgtaga ttccggggcc
    36781 cggacagtaa cggatatgcg cggcagagcg gttgaactgc cggcaaccat cgaacgggtc
    36841 gtcgccatcg acgatggttt tgtcgaaggg ctcctttacc ggttcggcat gcaggaccgg
    36901 atcgtcggcc tcggtggcac atgggttcag gattacacct accccttcga gggagccggc
    36961 ggagcgacgt atgagtacag gaacggcacg aacccggtca gctatcttat ccccgggctt
    37021 gccgatctcc cggtgcttgt ggagaccgga acggcgatca actatgagac ccttgcttct
    37081 ctcgacccgg atgtcgtcat tctccgcgag ggggcatggg cgttcagcgc cggatccgat
    37141 gaggctctcg gaaagacgat cgccacgata gattcacttg gtatccccct cgtcatcctt
    37201 gtcggcccgc ccttccagga gagcccgacc atcggtcata tcggtgagga gatcagactc
    37261 gtcggcgagg tcttctcgcg cgagaaggat gccgatgcac ttgcatccta cctggacgac
    37321 cagatcaaag agattcggag acgcacggca gatatccccg aaaatgagaa accccgtatg
    37381 atgcagctcg gtctctcccc acgcgcccgg cagggcggcg gtgccgggat ggcctggggc
    37441 ggcgatacga tcgagtctta cttcatcgaa gagattgcaa acgcaaagaa cgcctacagg
    37501 gggaccggag cgttcgtcgt cgtatcgacc gagcagatcc tcgcgctcga cccggatgtg
    37561 atcgtcctcc cgaccgccca gggctaccac ccggcaagcg agctctacac cgcaccttac
    37621 taccggaacc tgcaggaact cacggccgtg aagaaccaca gggtctacgc cctcccctgg
    37681 acacccttca actgggcgaa gcgccttgaa tacccgatcg aggcgatggt gatcgcaaag
    37741 gctgcctatc cagaccggtt cgcggatatt caggtgcatg aatgggtgct ggacttctac
    37801 cagaacgtct acggggtcga ccgtgcgacg gcagaggagc tccggtccgc ccagtggctg
    37861 gactggatgg tcgaggcaga gttctgaaac cctgccccat ccggaaccgg tatatgcccc
    37921 ggcgataacc agaccttcat ggaactcaaa gaacaactct ccggcgtccc cggtatgctc
    37981 cggccgttca aggcctacct ccgcgggctg gagcccggtg cgggcgacca ggtcgtttat
    38041 tacggctgcc ccggcacctg caccccgttc atcgaactcc tggggtttgc cgtccgcgac
    38101 ctccccgtcg agcaggtcta tgtgccctac gtggatgaga cggcggcaaa gattatccgc
    38161 ccggtcgggg gcgtcggcat gcaggtctcc ggtgacgcag cccgcgtcga cccagaagtg
    38221 gtcgtcctga tgggcgggct tgccatgccg ggcgtcccgg tcacaaaaga ggcggtgcgg
    38281 gcggtcgtcg gcgcccatac cggcgcaaag gtcgtgggta tctgcttcat gcagatgttt
    38341 gagaaagcgg gctggctcga cgcctttgac tttgacctga tcatcgatgc cgccatcgat
    38401 ccggtcaggg tctgggggtg accgccaccg ggtttacggc cggctgcggc tcccacggcc
    38461 ggtcggggcg gggcagggtg aaccggaacg ccgccccttc ctccgggtgg cccggcaccc
    38521 ggtcctcgac ccggatccgg ccgccgtagc gtgagatgag catcctcgtg atgtagagcc
    38581 cgagcccttt cccgctcccc ctgttcgtgc cccgcacaaa gcgggtgaag aggagaggtt
    38641 tcaccgtatc cggcacgccg ggtcccgtat cctcgaccga gacctcgacc gccccgtcac
    38701 tctcctcgac ccggatgacg acctcgacgc cgggattccc gaacttctcc gcattgccga
    38761 tgaggtttgc gaagacctcc gcgaggagat cgtcggccat gacctcgacg ggtcttccct
    38821 cgtagatgat cccggcatcg ggatggtgcg cgattccggc cctgatgacg gcgtcgaggt
    38881 cgacgggccg cgtcaccgtc tcgctggagc gcaacctccg gagcgtcgag acgttctgga
    38941 tgatctcgat gctccggttg accccgcccc tgagtttcaa ggtcatctcc ctctccttcc
    39001 cctccagcat atcggtgagc agatcggtgt agccgagggc gacggcgttt gcgttgttga
    39061 tgtcgtgcac catgatatcc aggtagaggt ttgcctcctc gtgggctgcc cgcagttccc
    39121 cttccacccg gatgcggccg gtgatatcgg agcccgaacc gatgatcccg accggtctgc
    39181 cgtcattgcc ggtgagcacc gtatcctgcc atgcgaggat gagctcctcc ccgctccggg
    39241 tgacgaccgg gctctcccgt ggatcggggt acgcggtgcc gctggcgatc gcggcatcga
    39301 agatctctcg ccgcgccttt tgatatgctt cagggacaac ggttgcaaac cagtctttcc
    39361 cgagcagctc ctcctcgcag tagccgagca actcgcaacc tcggcggttg atgagccgga
    39421 tagtatgatc ggcgttgatg acgacgaaga ggccgcccgc cacgtcgagg tagtgccgga
    39481 cgagatccct ctctttctcc acctcccgtt cccgcctctt gagctgcccg gagagggagg
    39541 cgacgacgag agcgatgagg acgaacatca gggcgcgccc ataatcgttc agtgtcagga
    39601 tatccggcct gtagaggagg ctgctcacaa cgacgagccc gccgaggaag agagcgacca
    39661 gcacgctccg cttctcccac cagagtgcgg tcaggatgat cgggacgtag aagaaatgcg
    39721 agaagacggt cccgagcatg aggacggcat ggaagtagta ggtcaggaag accgatatgg
    39781 caagaagggt gatgaggaca cccactcgct gctgtttggt aagggcaatc acgggggatc
    39841 caccttgact actgatatat ataattttaa aatataaaaa attcgctata caaatgattg
    39901 gccggttcta aaggtttatc ggcggtttcg agaggataaa agcgtcgccg ggagagagcg
    39961 gacataccgc gccggccggg gtttccggag aacggagacc ccggtccgaa aggtcgtgga
    40021 gatgcggtgc ggccctggat tgctctgatc catcccccgg aggaccccgt gcaagctcgc
    40081 atccggataa cgatgcggtt ttgcggaacg gggggtgagg cgctcccggc aatccacccc
    40141 ggactgtcac ggagaccgcg gatattcggg ttttagcccc gaaatgtgct aaaaactgcc
    40201 tatgtttaat acctcacacg ccaaacctat ctcaaccacc cgctttactg aagcggtaac
    40261 cgcaagaccc gcgccgcgcc ggcatccgga acatgccggc gaagaacgca tccgctggtg
    40321 aacccgtgtc aggagatatt accgaagcac tgaaagccat gggcgatgta gatatagcgt
    40381 cgtttccccc cggcgtcgtc gaatacctgc gagagttcaa gacgcaggag atccgcgaca
    40441 tcgtcgagac gctcctctac atctacatcg cccagaacgc ctcgccgccc cgcggagagg
    40501 ggacgggcgt ggtcgagcag agttgttacg cgtatacgtc cggaccgctc ttcgtgctcg
    40561 cagaggtcgg tctcatcgag tcctcctccc ggcatgccac gacgctggta cgggtgaccc
    40621 ctgccggaga agcgatcgcc cggccgctcg cggaggcccg tatccaggct ctggatcttg
    40681 gggcgatcgc ccgggagacc cacgagatcg tcccggtcct cctcgcggga acggtgaggg
    40741 ggtcgtatgt caacaagacc ttcccctcat cgctcccccg caccgaggag accttcatcg
    40801 gtttcctcct caacaacaac cccgcgctct ttgaagagtg cgagcggttt gccgcccgcc
    40861 tcagggccgc cgggtgtgct gtcctcgccc acgcctgcga ccccgacgga gcgggaaccg
    40921 gcagtgtcgt ctacgccttc cccccggagt ttgcgtacat cctgaaacgg atgctcgagg
    40981 cgatcgaccc cggtatccgg gagcactacg atatgctcct tgagtcgttc cactcgacgt
    41041 tcaccgttct gcgttatctc gcctgcggcg aggactacga ccggatccgg gcctcggcgg
    41101 cccaccgccg cgagctgtcg cggatgctct ccctcatcgc cgactcgatc tacatactcg
    41161 atgaggtgcg tgaggacgac accgtcggcc ttgcccggtt catcgtcaaa gaccgggacc
    41221 gctacgacgc ctaccttcgc gacctcgcgc gggctctgat ggccgacgtc gctcaaaaga
    41281 tcgtcatcga tcggcctgct ccggcggcag atccccggcc tcccgtagaa gaggccaccc
    41341 ccgccccggt gagagaagat gttatagcgg ccttgcctcc agctgacgat gagcgggagg
    41401 aggagtggga cgacgccatc ttcaccgacg aggatgaaga ggaaccgctc gcaccacccc
    41461 ctccgcccgc cccccgccgg attgtcgccc ctcgccccgc cccggagccc ccccgccccg
    41521 gcggtctcga catattcctc ggccatgcac aggacgggca ccgggtcgac tggtcgccgg
    41581 gcaggctcaa caacggtcac atgatcatcc tcgggggttc gggagccggg aagaccgaga
    41641 cgatccggtg tattgcaaca gaacttgcgg cacaggcgat gccggtcgtc ctgatcgact
    41701 tccacggaga tatggctgca accacgggag atatccggtc ctacaagatc cgcgagggcg
    41761 gcgagtacta cttcaacccg ctggaactcg acccggctat cgacgagatc acccctctcc
    41821 gggccacatc ggacttcgtc gacgccatat ccatcaactt cccgacgctc ggcatccagc
    41881 agcggagaaa gatcaagaac atcataaaag actgctaccg ggcctcaggg atcacgggaa
    41941 acaccgggac ctgggaacgg gtgctcgact tcgacgacat cgaaaacgag atcatgtcct
    42001 gtgaggatga ggcgatcccg gcatacctcg aggatatatt cgactacaag ctcttctcgg
    42061 gagaggagaa gatatccatc cccactatcc tctcgggcgg gatcacccac atcaacttaa
    42121 acgcccttcc cgagaacctg cgctacctct ttgcggatct cttcctccgg cgcatttact
    42181 atacccttca ggcgacgggc gagataccgc gcggggcgaa gaacgagcgg gagaagtttc
    42241 ggctcttcgt gatcgtcgac gaggcgaaac tcctggtgag ccagaagagc ggttcgaaga
    42301 cggcgatcaa ggcggtgctg aacaagtacg ctaccgagat gcggaagttt ggggtctcgc
    42361 tgatccttgc atcgcagctg atcgcccact tcaacgagga gatcctcgca aacatcgccg
    42421 tcaagttctg tatgcgggcc gaaaacaaga agcaggctca ggagaacgcc aagttctttg
    42481 aggtgagcga gaaggatctc ctcaacttcc agcccgggga ggggatcctg atcatcggtt
    42541 cggagaagat gaacgtcagg attgttcccg cgtccggacg gaaggtgtag gcccaaaact
    42601 cttatccatg gagccgccgg tgttcatgta tggatgcgct tgacgattac cggggaaaga
    42661 gggacttctc ccggacgccg gagccgccgg gagaggggac gggtgcaaga gatcgcccca
    42721 tcttcgtcat ccagatgcac gatgcgacca ggcttcacta cgacttccgc ctggaggccg
    42781 acggcgtcct gaagtcctgg gccgtcccga aaggaccgtc cgtgaaccca aaggagaggc
    42841 gccttgccgt ccccaccgaa gaccacccga tcggctacgc cgacttcgag ggcgtcatcc
    42901 cggcgggaag ttacggggcg gggacggtcc tcatctggga ccggggaact tacaaaaacc
    42961 tcacgcaaga gaacggacag gagatcgggg ttaccggggc gatcgggcgg ggccatgcat
    43021 cgatctggct cgaaggggag aagctccgcg gcgggtacgc cctcacccgc ttcaggaccg
    43081 ggaaaggtga ggcctggctc ctcgtgaaga tggatgacga agaagcggat cccggccggg
    43141 atccggtcgc gacggaaccc cggtcggtcg catccgggcg gacacttgcg gagatcgcgg
    43201 caggggtggg tgagtaggcc tgcgggacga tatcccggcc cggatatctc cgcgcgcgca
    43261 ttacggggct ccgatcgccc ggagggaggt ggggcgtgcg gcacccccca tccccgcctc
    43321 ccggcggtgt gcgtgatggc ggagtcggga ggtgcgcggt gggaagtgga ggtatttgat
    43381 ttccggaccg ggaaggatgg ccccgtagtc actgcccggt tgccttccgg ccgtggtact
    43441 ggccggtgca ggtgcaaaag atcgtaaatc cacatatata ggtttttcat accaagaata
    43501 aatcccgcgg ccgtaccgtg aaaagggatt tttctcataa tcccgatcgc cctccggtcc
    43561 ccgtagcggg gaccgcctga aaattttgat tccggaataa catcacataa agtgctacat
    43621 ttagtagatt atttacacac ccaaagacaa ataaacgacg caaagtcacc attttgtgaa
    43681 taccgattga atatggtgta gaatctttga tagaaagaga ttgctcggag gtaacgagga
    43741 aataacggct atcctccatt agtacgaaag ataagtttaa aatctgaact atcaaaaatt
    43801 acagggatca gatcgatcac gagatcttct cgaaggtgat tcatggcaag taaagtaatg
    43861 actaacagcg ctgtggcaaa cagcgttatg gcagaggcag taagcgacgc agagatcgtt
    43921 gcgcagttca ggcaacgcgg gtgctgggga ctgtacacga gcgtggacct gaaaggatgc
    43981 gacccggcat cgatcaggga tgcggaaaag attcaccggt tcatcatcga actatgtgac
    44041 ctcatcgata tgaagaggtt cggcgaaccg cagatcatcc acttcggccc gaacgagagg
    44101 gtcgcagggt tctccatgac ccagctcatc gagacatcgc tggtctcggg gcacttcgca
    44161 aacgagacca acgccgccta ccttgacatc ttcagctgca aggaatacga accctcgaag
    44221 gcggcggagt tctgcaagac cttttttgga gcagagacag cgacctacca ggtactgttc
    44281 agggactgaa tacgaataag cacccggcat atcctgacga tacgctataa cggcagagag
    44341 ctactgccat ctcccgccgg cgtaagccag aggtcgcggc gaggtggcca ctgccctcga
    44401 ttttttcaaa acttcgctct ggagcagcgt tttcggtagt ggtcctgcca tccgcctttc
    44461 tctcgagcgg agcagtcagg gcaatcgaaa aaagagagtt tcagggagac acgtaggggt
    44521 tccggaggcc cttcggggtt ccatccgcct tgagacctgc ggtcttacgt gcatccacgt
    44581 tcttctggtt cttcgtgatc ttctcggtgg cgagcttctt cacgatatcg aggccgggtt
    44641 tgacctggtc catcggtttc gtctcaaagt agcctgcctt catggcaccg tcccagacgg
    44701 cattgccctt cgtgctccgc acaaagaccg tgctccagcc gtcaggactg cccacggacc
    44761 cgctggatat gtcggcgagg tttgcgacgt agtccaggca gacgttgcag cccggctgca
    44821 cgtacttgtg gatcaacttc agaggcatct gggagactgc accgcgttcg gtgtagaccg
    44881 tgaacttgcc cttgccgata tccatcttcc tgacggactc catcttctgg ttgcagtggt
    44941 cctcgacgat cgcctcgagc gcctggtagg aaaggttctc catgcagaag atgccgagcg
    45001 cgagggcgat cttgtcgtcg acgtcgcgca tgccgatcgg atagagttga gccttccggg
    45061 tcgcctggat ctggcacggg gtgccgacga taccgacgcg gtcaagaccg tagctccggg
    45121 tcgcctcctt gatcagggac atgttcgggc tgatggtgta gcgcgttccg gcagcggcca
    45181 ggagttcggc cttcgtcgac acaaccatgg gctcaggctt ccagggctcg ttgcccgggc
    45241 ctgcgacgat ggcaccgtcg atgatgcctt cctcgagcgc ataggcaaag agcgtcgtga
    45301 tgataccgcc gtcctgggcc ttcttcagga tatccttatc cgtcgagcgt gccgcgatca
    45361 cggatttgta gttaccgagt acgtccattg ttctcacctc actgcatggc tcccttgatg
    45421 gcatccatga tgccctcgta gttgttcatg acgtcgaagt tgaaccagct cctggggcac
    45481 tgcgcgtagc aggcaccgca cttgatgcag aggtcgcggt tcacgttcgg tctgccgtac
    45541 tccatcgtaa tggcgcgaac cgggcaggtt ccggcacagg ttccgcaccc catgcagagc
    45601 ccctggttga tgacatcgta catcaggtcg cagccgcagg cctcgttgcc gcgctgtgca
    45661 agctgcatca gcggggtcag gtaggcggtc gcgagtttct tctgctcgtc gttgcccttc
    45721 agcagcaggt aggccatgac ggcgacgttc ctgactgcct gggggcacgg gggacaggac
    45781 ggcaggtaga gatcgacatc gacgacctcg ctgatgggca caaacgcttc ctgtccgggc
    45841 tggttccact ggccaccccg gcagaaccgg gtgatgttgc cgtaggccgc gcaaccgccg
    45901 taggcgacga gcaccttcga cttcgccctt gcttgcttca gttcttctac cgagatctta
    45961 tcgttcaggc agcacgaccc ctcgaccagg cacacgtcca tctcggggac gtggcgtaca
    46021 tcgaccaggg tcagtgcata gaccaggtcg gcgtaatcat cgagcagctt gaagagaccc
    46081 tcgtagttgt ccgcaagcgt cacgaggcat cccgtacatc cgctcaggtg taattcacct
    46141 atcgtaatct tctctgccac aggcttctcc tccttttgta caacttccac cttttctctt
    46201 tcaatcacat cagagggctt caccgcgggt ttcgcctgcg gccggctcgc ctcaggcttg
    46261 ctctccggct tcacctcagg cctgaccgcc ggtttcacct caggtttgct ctccggtttt
    46321 atctccggct tctccgctgg tttctcctgg cgacgcccaa agatgcgttc cttgatggtt
    46381 gatagcagcc ccatattcta ccccaatttc ctccagtacg atacgcactg ttttgggaat
    46441 ggcaccctca acctcctcag tgagccccag ttcaaactca tggctcgcga cacgcttcgg
    46501 ctggcacccg atgatcgtga tatcgatgac atcctttaat cgctgcagcg gttcggaaag
    46561 atcccacgaa tgggcatccc ggtacgcgcc cggagggaga tcttcaggcc ggagtttcgt
    46621 cacatcaccg gggtctgcgc cgaagtcggc gatatcgatg atgatcagtt tcttcaccgg
    46681 cacctccgca tcctccatca gcgtgaagag gaagtgaggg gcgccaaggc cggcatcgat
    46741 gaccttgacg ttgtcgggca gctgcaactt ctgaagttcc tcaacaactg cagggccgaa
    46801 cccgtcgtct ccgtagaggg ggttgccgca ccctgcgatc acgatctcac ggaatagcat
    46861 gcgtgtacgc tcactgaatg agtttctggg ccactaacct cttgtcttca tcgatcacca
    46921 gcatgtgcgt cgcacacgag acgcaggggt cgtacgcacg catgatgact tccgcgagcc
    46981 gccagggtgc gccggtcagc gcacggctgc aggtcgggaa gttccaggtg gtcggaacga
    47041 gcatcgagaa gtactggact cggccgtcct tgacccgtgc caggtggacg tcggttccac
    47101 ggggcgcttc gtttgctgcc catccgaggg atccgtcacc ctgcgggatg ttgtccgcga
    47161 gcaccgatcc ggaggtgtcg agtgcgtcaa gcgcatcgat gatgccgtag gcggtctcgg
    47221 ggaactccat ctggcgtgcg atctgcaggc ccatggcgcc tctcccgtcg tagttcctga
    47281 actggaccag gcgtgcacgc ggtccgacct cgaccggctg gccgtcgtag agcgggacgc
    47341 cggtgcaggc ctccatctgg ggccaggctt tggtgcctac agggctcgtg ccgccgatcg
    47401 ggtagttcgg gtcggcctcg ctgacctcta cctcacccat gtaccagtcc caggggcgga
    47461 cttcggtgaa ccgctcgggg aaccaggtgg gattctcatc aaggctcgag ctgccgtaga
    47521 ccggcgccgt cgccatgtag ccctggttgt ggtagccgag atcgtcgggg atcgggacct
    47581 gaacgccgcc gacctcggac cagtcgcggt tctggaagtt cttgaagacc gcgaccatga
    47641 actccatgtg ttcctgggca aggacccgtc cttccttcgc gaggtcatag atcttggcct
    47701 ttgcacgcgg ggtgatgttc ttgtacatac cgccgacacg ggggttgctc gggtggatgg
    47761 cctctccgcc gacgatctca ccgatggtct gaccgatctc acggagccgc tggatccgca
    47821 gagcgacgct cctgaccggt tcctccttcg tgaacgggtt gatcttcacg tcggtcccgg
    47881 ggatgtacat atccgggagg gagagtatgt tgtggatggc gtggctgtgc atcctgttgg
    47941 cacactgcag gatgtaacgc agaagttttg catcctcggg aatctcgcac ccgatggatg
    48001 cttccatcgc ctcaacgccc gccagggtgt gcgcgatagg acatatacca cagacgcgcg
    48061 atgctatctt tgggacctgc tccatcgtct tgccgatggc gagcttctcg acacccctca
    48121 ccggggtgat gctgagccag tctccacgct cgacgatgcc gtcgtcgtta accttcagaa
    48181 cgagcttcga gtggccttcg tgtctcgttg ttggggaaat ctctacaact ttcgacaaat
    48241 atatcgcctc attccgatat tatggtgttc tgaacacgat acctgtgtta ccacacaact
    48301 gtgtactgga aacgtacatg ggtacgttag aagggaatat gaagaaaaaa gagtttataa
    48361 ctttcgctcc agtacatccc tatagattac ggatttagaa tttcgtagca attattacaa
    48421 aaataggttg acaaattata aaaatgtaag aataatatag gttttataat gccccctcat
    48481 ccatctcccc gacgagttcg gctattgtcg ccttccgctc cgcgtatgcg ttgtgctggt
    48541 ggatgctctc ttcgttcgtc tgttttatgg tgaccacgga atcgccgggg agatcacgga
    48601 actgcgtgat caccttgcgg gccatctcgc ggacacaatc ttcaacgaac cgggggttct
    48661 tgtgagcttc catcaccacg taactctcgt cgccgcgctt gaggagctcg tagatgcgcg
    48721 cgctcatgga atccttcaag atcttgatga tcttctcgag gctgacgtgc tggtcgtcgt
    48781 cggtctcgat gcagaggaaa ccccgcccgc gctggttgtg cgtggccatc ggcacctcct
    48841 cgaagaacgc attgatcgta tcatccgcca caccgaggtt ctcgaggaca tgcagggcat
    48901 ggtccttcat gatgttctgg gcgcaggggc aggcggtcat cccggtaact tcggcaccga
    48961 tgctctttct gatgatcggg cttccgtcgt ttctctgggc tatcgcgctt gcgtgaacgt
    49021 tcaccacctc atggcagctc gtctcgctga tcggcgtctc ccgccggacc atgaactggc
    49081 tctgcatccg gacttcggtt cgctccgcgt actcgtgccg gtcgaggagc ttcctcgcca
    49141 ctgcgctgca gagctcctcg atcaccttca cctccccgtc gatggcctgc tgcagcacat
    49201 cgtcgatcac ctcaaagttc cgggagaggt ttgcaccctt gaggcttccc ggcaggtcga
    49261 cgaagacgtc gaaattggag atgaaaatga ccggccgctt gccgggtcgg gcgacttcaa
    49321 cgagtttttg aacgttcttc acacccaccc tggtcaggtt gatacggacc tccggcaggc
    49381 tggactggac atctggcagt tccattacaa atcctcaata gtgtatagag aataaggtct
    49441 ctcttcatca acattaaaat aaccccctgc gtgagggcag ttttcccata taatatttat
    49501 ctggtttgct gcccaaccgg gagtgagaat atatggtcca gaaatactac gagtccgatg
    49561 cggacccccg caccctggag ggcaggacta tcgccgtcat cggctatggc tcccagggcc
    49621 gcgggcaggc gctgaacctg cgtgattccg gttgtcaggt cgtcatcggc ctgcggcccg
    49681 gcagcagctg gcagaaggca tccgaagatg gctttgatgt gtattccgtg gcggatgccg
    49741 tcagacgcgc cgatgtcgtc cagatccttc tccccgacga gaaccaggcg gccgtctacc
    49801 gcgcggagat aggcccctac ttaaaagaga acgcttgcct gatgttctcg cacgggttca
    49861 acatccacta cggccagatc gtccccccac ccaccgtcga cgtgatcatg gtcgctccga
    49921 aggggcccgg tcacatggtc cggcggacct acgaggaagg gaagggagtc ccggccctca
    49981 tcgccatcca ccaggatgcc agcggcaaag cacgtgctat agcgctcgcc tacgcccggg
    50041 ggatcggcgc gacccgtgcg gtggtgctcg aaacgacgtt tgcggaggag accgagaccg
    50101 atctcttcgg tgagcaggcg gtcctctgcg gcggggtgac ctcgctcatc aaggcgggat
    50161 tcgataccct ggtggacgcc ggttacgcgc cggagatggc gtacctcgag gtcctgcacg
    50221 agatgaaact catcgtcgac ctgatctacg agggcgggtt tacgaagatg cgcgactcga
    50281 tcagcaacac cgcccagtac ggcgacctga cgcgcggccc ccgggtcgtc gggccggaga
    50341 cctacgcggc gatgcaggag atcctcgagg agatccggaa cggcgagttc gcgcgggagt
    50401 ggatgcttga gaacatggtg aaccgcccgg tcttcaatgc actgacaagg gcagacgagg
    50461 agcacctgat cgagcaggtg ggtaaagaga tccgttcgct catgccgcag ttcaaaaagt
    50521 aacacccctt tttttgcctg cggtcctgca ggcatcatgc cgcgccggat accatccggt
    50581 cttccgaact cttgcgcctt cgcgcaaacc cgcgacacga ctccggatgc ctctcgcgtt
    50641 gagtgcgttg ccttgaggag gagttgcaaa gacccgagca gggtttcgct cgaactaccc
    50701 ggtgcaggtg ataacgcggc ctcccgcaga ggtgtgaagg gggggtgcag gtgcccatct
    50761 accgaacttc gcgcctttcg cggtttcgca cgaactaacg agtatggatg gcgatacaac
    50821 ctcacgcgaa ggagagttcc cggcacctga gatcggactt cgcgcgaacg acccggtgca
    50881 ggcgacaata cggtctctcg cgaagatgca gaatccggta tcggggcgct gcttctccgt
    50941 attgatcgat gccggaacga gcaacgttgc cggcacccgt gccgaaccgc gcggaggcgt
    51001 accggacccg cgagcattgc cccggggttc cgcccgacac ccgtttatac ccgaccgtgc
    51061 cacccttccg tgtatgtccg tctgcatcgt ctttcactcg gagaccggca acacccggac
    51121 tgtcgccgag cggcttgctc ctctggtcgg cggcgatctc atcgaggtaa cggatcttgc
    51181 gcactactcg aagatcagca ggtatatctg tggcggccgc cgggctatga agggagaatc
    51241 cgccacaatc gagcccgcca ccatcgacgt taccgcctgt gacaccgtcg tcgtcgggac
    51301 tcccgtctgg gcgggcaatc cgacacccgc gatcaacgcg gcgatcgagg cgctcgtggg
    51361 tatcgagggg aaatccacgg tcgtcttctg cacatcgggc gggatgccgg ggaagacact
    51421 tgagacggtg acggcgatgc ttgcgggccg gggcgccgac gttcggggcg cggtcccgtt
    51481 cacggcgagg gatgtgcaga aggggaaggg agtggaggcg ctcgccgatc tcatcctccg
    51541 ggcaaagaga gggtttgacg catcagccga accgtcgagg tgagcgccgc ctcgagttcg
    51601 tcgggcgtga cggcgaccgt agaccggcaa catgcggtaa aaccggtggg cgcccatcca
    51661 cataagtttg aggtactcac tttttgacgt ggcgcgacca atactgaggc atgaccccgt
    51721 catacgaaga ccttctgaag aaggcatata ccaatatcac ggagccgacg gagtttgaag
    51781 atcggttcac ggttcccacc gcccgcgtgt tcatcgaggg gaagaccacg gttcttgaga
    51841 atttcgccga gattgcaggc atcctccggc gcgaccagga ccacctgatg aagcacctcc
    51901 tcggcgagct cggcactgcc ggcaaggtcg aggggacgcg ggccgtcttc tccgggaagt
    51961 tcgagcagga gcagatcaat acgatcatcc gcggctacgt cgaggactac gtcatctgct
    52021 cggagtgcgg taagcccgac acccgcctcg tcaagagcga ccggattctg atgctccggt
    52081 gcgacgcctg cggcggccac cggccggtca ggaagcggaa agcggttgca gaccccggtg
    52141 cggcagcaaa accgaccgag ggcgccatca tggacgtcac cccgcagttc ctctcgaagc
    52201 gcggagacgg cgtggtgaag atcgaccgct acacgatgta cgttgcgaat gccaaaccgg
    52261 gtcagacggt gaaggtgaag atcacccgga tcgccgggac gatcatattc accgagcgtg
    52321 ttgattaaga gatcaggtct tcctgatctc tacccacaat cctgcttctt cttcccggac
    52381 gcgggagtgg gcgaccccgc tcgccacaag ttcccggcgg agcaggtcag gggttgctcc
    52441 cccgctcctc cgggcaacgt cgcggtccca gtcggggttt cgcaggcgca tctcgcgaaa
    52501 tatatcgttt cggagcgcgg cagtcccgaa gctcccgccg acgaacgcgg caccgccggg
    52561 ccggagaacc cgctcgatct cgcggaacgc ctgcggccgg tcttcccaga agtagatcga
    52621 accgcggctg gcgatgagcg agacggcgcc gtcgcggatc ggcatgcagt ggacgtcgcc
    52681 gatgaccggg atcatccggt ctctgtagtc ggcagcggtc ttttgagcga tccgggccat
    52741 cgccggatcg gcatcgagcg cgatgacgga gagttcgctc ttctccacaa gggcggcggc
    52801 aaagaagccg gggccgctcc cgatgtcgag cgcgagacca ctctgtatac ccgaccacgc
    52861 gagcacctgc tctgcgatga ccgggtagac gggggcaaag acttcccggg cgatccgggc
    52921 aaagccttcg gcgctcttcg tcatgaggct ctacgcggcc tcaagggctt ccatctgccg
    52981 gaggagctcg ccggcgtagc cggagagccg ttttgccgca tcggtgattg caaggagagg
    53041 aggccggccg ccgatcgtgg tgacgaccag ttcggggtct gcgaaccgga acacctgccg
    53101 gtgctgcgcc acgtccacct cgggatcgtt gaggagttcg gcggcgagcg cgttgacata
    53161 ggtgatgcct tcgccttcaa agagtatccg ggccccatca tcggtcagct ccaggagttt
    53221 gagtttcatg gccatacgca ccctatgttt gggttcgggc ggggtatata ggtatggatg
    53281 acgatctgcc gcagctcgga ccgcatcccg cggagcggtg cggggcggag gaagggggcc
    53341 ggataaccct gcgcctcgtt cgttcgggga tgccggatga tcaccagcag aacgtctcca
    53401 tatcgcaacc ggcgtgcggc atccccggcg gcaccaggtg gctcatgtgg acgcaacggt
    53461 aatccctcgc cccgacgtcg cgggcgaacc ggaccgcctc ggcgtagttc atgtgcttat
    53521 cgatgttgta accctcggga gcgatgccgt ccacgaagag gaggtcgata tcgcggctga
    53581 ggaggaggtc ccggctcgcc tccggtatat ccttccgcgt atccgatgtg taagcgaccg
    53641 tgatgccgtc gcgctcgatc aggaggccgc aggtgtagac cggcgggtgg ttcacgggaa
    53701 agaacgtgaa ggtcacgccg aagaggtcga acggttcgta gaccgggacc ggatgcttct
    53761 caaacgggag gaagtggagg tagcgggagc agtaatccat caccgggggc gccgcgtagg
    53821 ccggcggcac cttctgcacc cggtaaaact caccaaaccc gatgaagtga tcgtagtggc
    53881 cgtgcgacca gatgaccgca tcgatatggg gggagcaagc ccggaggagc tgctggcgca
    53941 ggtccggcga cgactcgata aggatgtgtt ttctctcaat ctcgatgaga aacgacgtcc
    54001 ggagccttga cctccccgcc gccgtcatcg cccggcaggt ctcgcagtcg caacccacct
    54061 tcggcgtccc gatggcatcg ccggtcccga gcagcgttat ccgcatatca gtatccggtt
    54121 ccgcggatga cattgcgttc ctcgaccctg aagaggccct gctcgatatc caccggatcg
    54181 aggcggcggc ggtcctccat cagcgccgat atcactgcct ccttcatcat catccgtata
    54241 tcagagcccg agaacccctc cgtccgggcg gcgaggtccg cgaagtcgca gttgcagtcg
    54301 atcgtagccg cgaccttctc gatgatcgcc tgccgcatcg cctgatcggg gagcgggaac
    54361 tccaccactt cgtcaaaccg ccgccacgct gcctcgtcga ggatgcgggg atggttcgtc
    54421 gccccgatga ggaggacgcc gttcttgacg aaactgatca ggtcgatgtt cttcaggagc
    54481 atattcaccg cacgcttcat cgcaccgtgg tcgtcgctga cccgggtctt tgcgacgaag
    54541 tcgaactcgt cgatgaagag aatgcaggga gagagcctct ttgcaaggtc aaatatccgg
    54601 tcgatgttct ttgacgtctc gcccaggtac tgggaggtga ccatagcgag acggacctcg
    54661 agcaccggca tgtggagtgc ccgcgacatg gcaagagcaa gcgatgtctt cccggtgccg
    54721 ggcgggccga caaagaggag ccggccgaac tcgtagatgc cgtgctcttt taagaactcc
    54781 cggtgctcga gggcgacagc gatcttccgg atgacctcct cctgccttgg ggtgcagacc
    54841 agtttatcga gcgtgaactc gatctcgtcg ggtgcactga tgatgaggag gtcgcgcgcc
    54901 tcccgcatct cctcgctccc ggcgatgatc cgggagactt tcgcctctat atactccttt
    54961 gtatcctcaa accggggaat ttttgcccgg acatcccggt aactgacggt gataccatcc
    55021 tttccttcaa aatagcatgc aagggcgggg ttctccccga ccctcgagac cccgcttttc
    55081 ttgaggaacc atccagccgc tgcatcgagc gaagggaggc tcaggcgctg cccgaactcg
    55141 tcgtagccga cgaacgggtt tgcgcgaact gcctggcaga cggcatccgg gatgccgagc
    55201 gtcttcttga tcgccccctc gctcacgacc aggggacgct tcacctcggt gctcccgccg
    55261 ctcccggcgc cgaagaactc ccggtagcgc gggtggaggt cgttgatgtc gagagcctcg
    55321 ttccggttga atatctccgc ggtgaggagg agttccatga cgacgaggac atcacgcgta
    55381 cccgtttcgg tggtcatgtc tatgagcaga catattggcg cgcggtatca ataagcataa
    55441 ctcaccgggt ggtgacctcg catccgtcgg ccgtgacgat cagggtatgt tccgcctgcg
    55501 agacgaagga gtccgggata tcgtgcagca cagggaaggg gtgaaggatt ccggtgcgta
    55561 cgagcgtcgc aagccctatc tcgactttgt cgcccgggac ccagcggcgg gcgaagggga
    55621 ggccgcgccg cggccgtgcc acttcaagga tcctctttgc cgacggcagg cgtgcgggac
    55681 gggctgcgat ctgccggtag atctccacgc gcgccgcctc gctgatcctc cccgatccgg
    55741 tggtcgcgaa gggctcgatg gcaaagacca tcccttcctc gatgacggcc ccgccgctca
    55801 tcgcgacgtt cggtatggtg ggcctggcat ggaggttgta gcggtcgagg ccgtgcccgg
    55861 tgaggttcgc caccggtctg tagccgcgct cctcgatcgt cgcctggatg atcgccccga
    55921 gctccccggc ggcgaccccc ggacggacgg cagcgatcgc cgcctcgagg gctcccgcgg
    55981 aggcctcgac gaggggcgcg tggtcgccga ggtcgacggt catggcggtg tcggcgatgt
    56041 agccgtctat caggacacca aggtcgacct tgatgaggtc gccccgcgag aagacccgct
    56101 cgtcaccggg catcgcggta tcgtgggccg cggcctcgtt gaaggagacg ttgaggggga
    56161 aggcaaggag cgccccctcc tccatcacca tagcctctgt ggcttcaacc atctcaagga
    56221 ggcctgcccc ctccttcacg agccccgcgc cctggtggag cacctttcgt gccagcgccc
    56281 cggcttcgcg gtaggcgtcg tagacaacat catccatgat cataaggtga gttcctcttc
    56341 gtattgggtg aaccggtcga agtcggtctc cgtcaccgcg cccatatcct ccaggcggac
    56401 gccgccgatg cccgggtagt agagtccggg ctcgatggtg acgacgtttc cgacagcgag
    56461 cgctcctccc cggggcccga gcgacggctc ttcgtgcacc tcaaggccga cgccgtggcc
    56521 gaggctgtgg atgaacccgc gcgtatcgct ctcgtagccg cggtcgcgga agaactcgac
    56581 caccgcgttg tagaggtccg cgccggcggc cccggcccgg acttctgctg cggcgagggt
    56641 tttagcgtcg cggacggcgt cgtacatctc ccggatctcg ggcgagggtt cgcccttcac
    56701 gaccgtccgg gtcatgtcgg cgtagtagcc ggagagttcg tcccggggga agatgtcgat
    56761 gacgatcggt tcgttctcaa ggagcgggcc ggagcctgtg ttgtgcggga gggcggtctc
    56821 ggggccgcag gagacgatgg tgtcgactcc ccggcacccg tgggcgagga ggacggtatg
    56881 catcgccgtg cggacggcct cagacgtgag gggggcgcca tcccggcaga ggacgccagc
    56941 ccggggagcg gaggagcgga tgagcgtgac tccccggtct atagcggcct cggcggcccg
    57001 ctggacggag cggatgcgct cgatctcctc cggtgtcttg accgctcgca tcccctcgac
    57061 ggagccggca ccgtcgagga cgaccggatg gaacgtctcg agttcccggg cgagggcgag
    57121 cgggaaggtc gccgggacca ggacggggcc gccggcaagg tccgcaatca cgtgggcggt
    57181 cgcccgccag cgcgattccc cggccttgat gtactcgagg taaccggcat cggcgcgggt
    57241 ggcgaccgcg gcgatcgatt cccggaccgc tcgctcgtgc tccatctggg ggacgacgat
    57301 catcccccgc tcgccgggct ttgccacgta gatcacgggg tcggtcgtgc ggaaccgggt
    57361 caggtagcgc acgttggcat ccgccgacga gccgtaagcc gcgtatgctg ccgccccggc
    57421 atcccggata acagaatcca gaccattcat tctcccacct ctttcgttga aaccacaagt
    57481 acttttaccc ccgcgttgaa gtatgaatga tggatgaggc aaccaaacag gccttcaagg
    57541 gcaggttcgt catgctgacg gtcatgctga acatcgtcat cctctgtttc gccatggccg
    57601 cctttgtcct cctccggttt gcacccgagg gggccccggg tctcgtgatc ggggttatcc
    57661 tcctcgccat cggggcggcg ttctccgtat cgttctggaa gcgctacacc ctgacgaagg
    57721 cctggctgca cgagcagccg taacatgcgc gaatgatggt acatcggcag gcggatatgc
    57781 tgaagaagtt gaaatctgag atcgaacttg cctcacggca ccttgaggtg atacgggcgg
    57841 tcgtggagca ccagcccatc ggcatcatga agctctccga gatcctcgac ctcccctacc
    57901 accgggtccg ctattcgctg cggatcctcg agcaggaagg atatatccgg gcatcgccgg
    57961 cgggcgccgt ggcgaccgcg ctcgcggccg acctcctttc cggcctcgat gccgagatcg
    58021 gcgaactgat cggccttctc gtgaccgtcc aggaggagaa ttcccgtaat ctttaatacg
    58081 gttcagacga gacattatag agcacttatt ccagtgccgc cataactcag ttggtagagt
    58141 ggctggctgt taaccagcat gtcacaggtt cgagtcctgt tggcggcgct ctgttatttt
    58201 ccggttatct cctctggttt cttattcagt agacaggctg gttgtttgcc agcatgtcac
    58261 aggttcgagt cctgtcgttg tattgcataa ctccgaacat tcaatccaga gaatgccggg
    58321 tatccctctc gctcccccat acccaagaaa agcatgagag gaatgcccgt accccgatac
    58381 tcgctactga gtgcccggcc ggccccctct cctcccccct ttcttctcca ggcgtgtccg
    58441 cctaaggaga accgccgccg gcccgtaccc ccgcccctct cgccgggcga gttccgcccc
    58501 ggcggggacc agagtgccgg agaatgcccg ggcgaggatg gactgccgca tcgtcccggt
    58561 cttcgcgcgt gccgcagcga acccggcctc gatccggacg gaaagggtga acgggctgcc
    58621 gggggtcgtc gtcccgcccg gtctggcgcc gggatcagca cccgggccag gaaggggcga
    58681 tcacctgcat caccggttta tctcgcggga ggcagtgccg acgtggggat cgaacgggga
    58741 gaggggcgtt tcccggaggc tcccggcagg cgtaccgggg ttcccactct ccagcagtgc
    58801 atgccctttg caccgccaca cccgacctca tgaagagaga tcgctcgtag tgccgggtta
    58861 cctgctccag ggtgactgac cggagaccag gaacttctcc gagtggcgtc ccctccggcc
    58921 ggggtcctgc tcccctgctg ccacctggca ctcagcgctg caaaatcgca cgttgccgcg
    58981 cgcgtcttcc tgtttcggcg agaattcttg cccacagtgt tcacaataga tcgattcagg
    59041 aatgatagat cacccgaagg tggaaaggag gttccagggg ataaattttt cccctggttc
    59101 cagaagatac cctcacagac ccaggacatc ctccccgatg tatccgggag agacatgccc
    59161 ttaacaggga gggtcaacca gaagacgcaa catcccgcag gtaccccata gagggctcgt
    59221 gcagcactgg cgggcatcaa cacggaagca aaccttccta accccggagg tccaattcca
    59281 tgcatcgatg ggtgatcgat gtgccgaagc tcgtactgat cgatacagag accgggatga
    59341 gggtccggca ccggatccgg agcatcaagc aggccgtaaa acagcaggag tggtacgagg
    59401 cgaagctcgg gcggcgcatc cggatcgaga aggtatttga cccataacgt ccctccgggc
    59461 ggagcggctg aagggctcac ccccttatct cagccacccg ggtctgtaga agatccaccc
    59521 gaccacgaac agcgatatga tggtccaggc ggtcacctcc cagagataga gagtgaaggg
    59581 ggcgccgagt atgttcgtcc cgagcgtctg cccgatcagg gtcgggatca gcaccagcgc
    59641 cgagaagacg gcgatcaccc gcatcgcccg gttcatctcg taggagacgg cgttgatgcg
    59701 ggcatcgatc agggagaaga ggccttcacg gatgttcccg gcggtctcgt gcaggtaagc
    59761 cgtctcatcg gcgagggcat caaaaacgct cttctccccc gcccacggcc ccatcccggg
    59821 gatgttccgt gcgatgaccg ccatcacctc cttctggtgg aagaggcttg aggcaagggt
    59881 gctgctcacc cgcttgaact ggaaggcagt atcgaggagg ttttccagca cctcttcggc
    59941 gtatcctacg accgccttcc cgcagacagg tgttcttcaa cgcaaaggtg cattcactgg
    60001 tgtcgtgccg tatcccgggt ggccgtaaaa aactacgtga tgcccgtggg gccacaggca
    60061 taccggatga aaattgcgga gggcattt
//
LOCUS       DAOSEE010000002        42258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_516_length_42258_cov_240.815914, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000002 DAOSEE010000000
VERSION     DAOSEE010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 42258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42258
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_516_length_42258_cov_240.815914"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(306..1052)
                     /locus_tag="PLI76_00320"
     CDS             complement(306..1052)
                     /locus_tag="PLI76_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome assembly chaperone family protein"
                     /protein_id="HOI60502.1"
                     /translation="MEDVVVTFFRDDAGGAPVLVEGLPGIGHVGKLVADHLIDELGGE
                     KIAEISSVHFPPQVVVDEQGVTHLVNNEIYRCEKDGVAILFLVGDFQSTSAEGHYVLA
                     ERYLDLAEALGVRRIYTLGGYGVGHLVEHPRVLSAINMERLRPEVEAAGGSFENAGSG
                     GMIMGASGLLLGLGKARGIEGICLMGETSGYIVDPRSADSLLTVLSAMIGIDIDHTTL
                     QQRAEAMEQIIANIRETEEARGREELNYIG"
     gene            complement(1034..1201)
                     /locus_tag="PLI76_00325"
     CDS             complement(1034..1201)
                     /locus_tag="PLI76_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-protein complex protein Nop10"
                     /protein_id="HOI60503.1"
                     /translation="MSNRIRRCPDDLRYTLARVCPICGRSTRPAHPARFSHEDRHGDY
                     RRVMRRWKTSS"
     gene            complement(1208..1987)
                     /locus_tag="PLI76_00330"
     CDS             complement(1208..1987)
                     /locus_tag="PLI76_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     alpha"
                     /protein_id="HOI60504.1"
                     /translation="MHEREWPEEGELVVCTVADVKDFAAFVTLDEYDGRRGLIPISEI
                     ARGWIKYIRDYIREGQKVVCKVLGVDPGRGHIDLSLKDVNEHQRREKIHEWKNEQKAE
                     KWIGFASESAGVDRKVIEESIYREYGLLYPALEDIVTTGGEAAERLGLDEPVKEALIT
                     IANENVKVSKVTITGHLVLTSPRPDGVNVIRRALRSAQPKIEDVDIDLIYIGAPKYRI
                     KVTAPDYKAAERAIEKAASAAVGVVERAGGSGKFVRKQKAG"
     gene            complement(1995..2183)
                     /locus_tag="PLI76_00335"
     CDS             complement(1995..2183)
                     /locus_tag="PLI76_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S27e"
                     /protein_id="HOI60505.1"
                     /translation="MVRLNRENRSTFLRVKCPDCENEQIVFERASTVVACSVCGRILA
                     EPHGGKADIKADILAVLE"
     gene            complement(2192..2470)
                     /locus_tag="PLI76_00340"
     CDS             complement(2192..2470)
                     /locus_tag="PLI76_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L44e"
                     /protein_id="HOI60506.1"
                     /translation="MKMPSKFKTYCPFCRNHQTHEVVKVKRGKVRHFNWIDRQKDRRS
                     MVGNMGKFSKVPGGDKPTKKINVKYRCTVCGKAHLRPGFRIGKFELTE"
     gene            complement(2508..3170)
                     /locus_tag="PLI76_00345"
     CDS             complement(2508..3170)
                     /locus_tag="PLI76_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60507.1"
                     /translation="MAADPLERLRQTLLEMHHSGRLSDIEPALYEGARAYVEKLKADY
                     YGLPNPLESQSGSLLIEEIGSITETVQEIFAIRTRRILDLAFQQIEGQYFDRGEARKM
                     LPAERAMYGQIIDAVEGCRETLIRGEEGSFQGGGAAAVDAIEEPDIPPAAGAGRPAPT
                     IPRPLSSPYALVHIRSDVESFMGVDGRVYVLTQGDIVTLPENNADVLCEHDIALNIRL
                     NK"
     gene            complement(3174..4289)
                     /locus_tag="PLI76_00350"
     CDS             complement(3174..4289)
                     /locus_tag="PLI76_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase small subunit PriS"
                     /protein_id="HOI60508.1"
                     /translation="MKPATIEFVKQRFAEYYQRQTLAAPSSLEQREWGFVFFDAASAV
                     RMRRHMAFADPLELTAYVRSLIPAHAYYSTAYYQTPSAPTMNDKHWAGADLIFDLDAD
                     QIVRGPYAMMLARVKEETVKLVGMLTDELGFARNHIRIAFSGGRGYHIHVTDIAVRGW
                     ESPERREVVDYVCGIGLDPGVMLAPGRTPAGWRWRYIEALRDHLLWLEGLDAAEAAAY
                     LTGPAGIGKRAATEFLAGLGALREKDPGTLRENRIVRAIMGTPAFEERVRGAGARADE
                     PVTTDIKRLIRAPGSLHGGSGMRVVPLDSRDLAEFDPLVDAVVFGDREVRVDLQMNVA
                     TSLLGNTYTLKTGPASVPEALAVFLCCRSMAEIAGGA"
     gene            complement(4286..5860)
                     /locus_tag="PLI76_00355"
     CDS             complement(4286..5860)
                     /locus_tag="PLI76_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HOI60509.1"
                     /translation="MNDTDTLREIISRILSAGPDPADIQRIKLEVCREHGANMPRNSA
                     VLAAATPDERERLRPLLLVKPTRTLSGVAPVAVMTSPHPCPHGKCLPCPGGPEHPFGS
                     PQSYTGEEPAALRAREHAFDPYRQVQARLGQFEALGHHVDKAELIVMGGTMTARSVEY
                     QEWFVGAAVQAMNDYPRAGVPPEKPDLDAIFAANERAGVRCIATTFETRPDWCRAEHI
                     DRMLAMGVTKVELGVQQIDDRILDYNRRGHLVADSVAANCLLRDAGLKVGFHVMPNLP
                     GASMEDDRRMFRELFADPRFRPDFLKIYPTLVTPASEIEALWERGDYRPYTEEELVDL
                     VAYAKSLLPEYVRLQRIQRDIPAKLIVAGSRHSNFRQLAAARLHSQGLRCRCIRCREV
                     GRSVAPEETALSTVAYPSCGGEERFIQAGSEDALVGFARLRFPHRPFRDELAGAALLR
                     ELHVYGSVVPLGTPAAADEWQHRSYGVRLLAAAEEEASGHGYGRLAVMSGVGVRPYYR
                     KQGYERVGPYMIKTIS"
     gene            complement(5864..6130)
                     /locus_tag="PLI76_00360"
     CDS             complement(5864..6130)
                     /locus_tag="PLI76_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0058 family protein"
                     /protein_id="HOI60510.1"
                     /translation="MQKEELLHLHMLLVHVRKYYESTTGEDVSTDQYNALHISPVHIH
                     KNKVTHKKAILTLGNEIVQHIRANHNPYIDYHADFPSGRIATEH"
     gene            6198..7070
                     /locus_tag="PLI76_00365"
     CDS             6198..7070
                     /locus_tag="PLI76_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOI60511.1"
                     /translation="MHKVRAVGAFIGLAIGDALGAPLEGFPPPPFTVTEMEGGGMHHT
                     LPGQYTDDTLQASALAETLVRCGGLDPGDFSRRLISAYRARPEFFGPTSRAVLDLVEG
                     GVRPEDAARMVHDARGGSRSNGSVMRGIPVGIFYPPDVVREASLAASRVTHFDAAAGE
                     ASAFVNRMVSGMCRGEEASAAFGYALAACRDPELHGLLRDWRAHPREPSLDAVLCTHC
                     AVSVFMDAPTFRDAVITAVNLGGDADTVGAIAGGLAGACWGLHAIPGSWLSALQDREM
                     LLDLARRLSQAGRP"
     gene            complement(7201..7653)
                     /locus_tag="PLI76_00370"
     CDS             complement(7201..7653)
                     /locus_tag="PLI76_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0179 family protein"
                     /protein_id="HOI60512.1"
                     /translation="MVKPKTKVTLIGAALAKPGLDFVYEGNVCTECESCKVRKTCHNL
                     QPGKKYRVAAIRSNTRQDCPVHHEAVVAVDVLEAPIVALIGADMAIANSKISYEFSCP
                     RTTCRSYRLCRPDGIIEGEKYIVGEVLGNAPDVCERGRPLKLVELRPI"
     gene            complement(7662..8744)
                     /locus_tag="PLI76_00375"
     CDS             complement(7662..8744)
                     /locus_tag="PLI76_00375"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-1-phosphate
                     dehydrogenase"
                     /protein_id="HOI60513.1"
                     /translation="MSADGINLLRTKVFDKSKWMQLPRDVVIGHDVIEQLPTVCEDLA
                     LGDSVLIISGDRTRDLAGRRVEALLAGSYDVATCAADPNNPVETIKRAEASAAKAGFV
                     IGVGGGRVIDTAKIASYNTDRHFISVPTAASHDGIASSRASVPTADGNVSLAAEPPIA
                     VVADTAIIASAPHRLLASGCADIMANHTAILDWELSHRLRGEALSEYALTLSRMTAEI
                     LFKNADLIKPHSEESAWIVTKALVSSGVAMSIAGSSRPASGGEHKFSHALDRLAPGKG
                     LHGEKCGIGAIITMYLHGGDWEGIRDSLKKVGAPTTPAEIGIDDETAVEALLAARTIR
                     PERFTILDMGLTAASARDLVKMLYRE"
     gene            8756..9601
                     /locus_tag="PLI76_00380"
     CDS             8756..9601
                     /locus_tag="PLI76_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF63 family protein"
                     /protein_id="HOI60514.1"
                     /translation="MIREFLYKYYIDPIRYGQPYTLVDTLTYALILIAAVYIVYRGLR
                     RYKIAVDDELVLATMPFVVLGGLLRVVEDTGMIASDFRFLLITPLIFFTIFAVAAVSL
                     FLGKLAENAGFVARYSRVYGGAGIAACIVAGAALVWFGLTETKIALDVLAAILALASV
                     SSLALWALLAYGAKWDYASNIFYKLLIFGHMLDASATSYGIEIHPIHYVEQHVVGSGL
                     IEATGTAFSMFLLKIVVLIPAIYILEQYRREGNPGLWHLILLAMITVGMAPGVRDMVR
                     MVLYV"
     gene            9594..10160
                     /locus_tag="PLI76_00385"
     CDS             9594..10160
                     /locus_tag="PLI76_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HOI60515.1"
                     /translation="MFERPIVGATIVAAALFLVSVGLGIVAVIQDPSTGAQVMTLFRD
                     QVVDQLLSDSPAVLAGKIFVNNLGTCLLLFLGGASLGIVTLIVLTVNGLLIGAVAELV
                     RQQQGLLFIAAALAPHGIFEIPSFLIAGGLGFLLGQELIAEWHGRGDAAAGALPLARL
                     FLRVVVPLLAVAAIVEAFITPAVLSVIT"
     gene            10197..11630
                     /gene="proS"
                     /locus_tag="PLI76_00390"
     CDS             10197..11630
                     /gene="proS"
                     /locus_tag="PLI76_00390"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOI60516.1"
                     /translation="MEEDTGALPRKDDFSEWYNEVLWRAEIMDVRYPVKGLYVWYPHG
                     FAIRKRAYGILRDLMDRDHAETMFPLLIPETEFMKEATHIKGFEDEVYWVTHGGRNEL
                     DVPLALRPTSETAIYPMYSLWIRSHTDLPLKLYQIVNTFRYETKHTRPLIRLREITSF
                     KEAHTVHATREEAEAQVETALGLYREFYESLRVPVIISRRPAWDKFPGADYTIAVDTI
                     MPDGKTLQIGTAHMLGDHFSRTYEITYEDANGERQYASQTCYGISERSIAAVISVHGD
                     DKGLVLPPEVAPIEIVVVPIIVGKRRDEVLLAARALEEELRSAGFVVKLDDRDLRPGA
                     KYYHWEMRGVPLRLEIGPRDIDANTVVAVTRDGHKITLDRRGVVEGVFSALAAFRKGL
                     EQTARQAMIDRITVAATLEETVLAVKKGVAVVHWCGSKECAEEIEAAVDASILGSDIR
                     SDLITVSRGPCIACGGDGTSTLVARTY"
     gene            complement(11631..12086)
                     /locus_tag="PLI76_00395"
     CDS             complement(11631..12086)
                     /locus_tag="PLI76_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60517.1"
                     /translation="MTSRMHTPHTTCPGCHEEVFLDELVGGRCPLCGYSLDEDEGVCS
                     EYEETLERSDLGWMILQFFIFRRFCNEGANPLQVMQVLSRYEELAQCNPADAEKMQFT
                     LEVPMSRWERLLPKRCSKCGRIFFSGGKAAISGDISSPEHPREYTCPSC"
     gene            12445..13365
                     /locus_tag="PLI76_00400"
     CDS             12445..13365
                     /locus_tag="PLI76_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOI60518.1"
                     /translation="MASPAASEVYIIDASDRDHAIEALWREVGFPSLDGKDIAVKANF
                     NSDDPFPATTHPDTLGAIFGQLLDAGARTIRLGERSGMGETRAVLRNRGAVDVAARAG
                     ADVVALDDLPREGWEAVPGDGLHWREGFLIARLFWDADVVVQTCCLKTHRFGGHVSLS
                     LKNPVGAVAEKNPGGGYNYMMELHASPHQRRMIAEVNRFFPCALSVVDAAEGFSTGGP
                     ERGHRIAPGVILASTDRVALDAAGVALLRLYGSTPDVMRGRIFAMDQIARAAELGVGV
                     RSAQDLRLVALDAESKETAFDMRRILDGAG"
     gene            complement(13362..13913)
                     /locus_tag="PLI76_00405"
     CDS             complement(13362..13913)
                     /locus_tag="PLI76_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOI60519.1"
                     /translation="MEFATTLLIAVGLAMDAFAVAASGGTTVREARFRYALVVGALFG
                     GFQAGMPVLGWLGGAGLASFVGTYDRWIAFLLLALVGGRMIAGAVRGDGEAVQLATVG
                     IATLLLLAVATSIDAFAVGVTFAVLGTPVLWPAVVIGIVTFALSAAGVLIGSTLGRAF
                     GQKVEVLGGAILIGIGLRILLGL"
     gene            14005..15912
                     /locus_tag="PLI76_00410"
     CDS             14005..15912
                     /locus_tag="PLI76_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HOI60520.1"
                     /translation="MSTQKIGLIQTAVGDDLDRNLAHALALTKQAIAAGARVICLQEL
                     YRTPYFPQYEGRDASFYAETIPGASTDAFSALAREHGVVIVVPLYERTADGEFYNTAV
                     VVDADGHLLPPYRKVHIPYDPLFYEKTYFRPGDRYRVYDTRYGRIAVLICYDQWFPEA
                     ARAVALQGAEIVFYPTAIGRIAGEEEPPEGDWRDAWETVQRGHAIANSVHVAAVNRVG
                     SEGDIRFFGSSFVADAFGNVLARAGETAEEILVVEVDLAGNEAVREGWGFFRNRRPET
                     YGAITRRFLPAKTPQALGYRMPAEWEPHDAVWLSWPHDRETFSDLAAVERAYVAIIAA
                     LRGSETVDLLVTSDMMRTRIKAMLEEEGADTGHVRFHIAEYADVWFRDYGPTFVVDRR
                     TGGLAMVNWTFNAWGEKYRELMGDTRIPLIMNREMNLPLFTPGIVMEGGSIDPNGRGT
                     VLTTEACLLNPNRNPHLSREEIEAYLEAYLDAGHVIWLKQGIAGDDTDGHIDDVARFV
                     GPTTVLCALEENEDDENYAVLQENYAILRASTDQDGNPLTVIPLPMPGAVDGEDRLPA
                     SYANFYIGNTVVLVPVFGHPNDETALARIRQAFPDREVVGIDCTAMVAGFGAIHCISQ
                     QQPSVQRPESK"
     gene            complement(15924..16433)
                     /locus_tag="PLI76_00415"
     CDS             complement(15924..16433)
                     /locus_tag="PLI76_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOI60521.1"
                     /translation="MDALEAIMTRRSVREYTADPVPGETVRRLLAAAMQAPFAGDRPP
                     WHFVVIDDPRILARIPMIGPYARVSPPPPLAILISTDLAPPESPGLQVQGSAAAAQNL
                     LLAAHALGLGAVWTAVYPDADRMLGFANLFSLPGTVIPFALVTVGHPAARPPPVDRYS
                     EDRVHYNGW"
     gene            complement(16449..17168)
                     /locus_tag="PLI76_00420"
     CDS             complement(16449..17168)
                     /locus_tag="PLI76_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOI60522.1"
                     /translation="MDPEILSIGLLLAAVLAAGCAGNLFLPGKNSTPAPPDDAVILPA
                     PRSEGGVSVEEALWKRRSARIYAEVPLMLADVGQVLWAAQGITNDRGYRTTPSAGALY
                     PLEVYVVAGSVVDLEAGVYRYRPDGHLLVRIGGGDRRADLQAAAVDQASVGDAPATLV
                     IAAVPERTTAKYGERGTRYVAMEAGHAAENVYLQAAALNLGTVAIGAFDDDRVEEILM
                     LPKNATPLYLMPVGRPVPGND"
     gene            complement(17279..18751)
                     /locus_tag="PLI76_00425"
     CDS             complement(17279..18751)
                     /locus_tag="PLI76_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI60523.1"
                     /translation="MVFETKESLTDEEIRRGLKLVIRDGLATQAMVTLTGGIFLVAFA
                     LQLGASNTVIGLLAAIPPLAELLQMPAIYVVDRVQKRRLVVVAASLAARLCWLPIVLI
                     PFFFSPEQGIFVLIASIALYASFSAISHCGWNSWMRDLIPQDRLGAFFSRRLTLSTAL
                     ALVISLAAGFFIDSWKTVFPDLAAYGYSALFLLGLVAGLFGITLLARTPEPRMVAADE
                     EDGLLATIKKPFTDVNFKNLIIFLGSWNFAVNLAAPFFTVYMLQTIGLDISVVVALGV
                     GSQIMNIVFYRSWGRISDRYSNKSVLAVSGPLFMLAIFAWMFVTLPDVYILTYPLLVL
                     IHILMGVSLAGVSLASGNISLKMAPRGQATGYLAASTFTNSVAAGIAPILGGLFVDFF
                     AERELLWTLIWKSPVRELVFVTLDLRQWEFFFLFAFILGLYSLHRLAAVQEEGEAKEQ
                     EVVDALIAGVRRDMRNFSPAGGLRDLVKFPFSSARGRKKR"
     gene            complement(18991..19803)
                     /locus_tag="PLI76_00430"
     CDS             complement(18991..19803)
                     /locus_tag="PLI76_00430"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HOI60524.1"
                     /translation="MRRFEGKNVLVTGGSTGIGRATAIRFADEGANVAINYYASETEA
                     EITLEEARKTCSIIRGKGCGEMLVQGDVAHEEDVKRIFREVLGAWGRLDILVNNAGVQ
                     VASPAHETDMDAYDRVLAVNLRGAFLCAREAVRHFLDRGGGGVVLNTTSVHETIPKPY
                     YAPYVASKAGLAALSRTLALEYAGQGIRVNTVAPGAIATPMNREWMDNPARRAEVEKH
                     IPVGRAGLPEEVAAVFAFLASDEAAYITGQTLYVDGGLTLYPDFRVPWSSGS"
     gene            20127..20513
                     /locus_tag="PLI76_00435"
     CDS             20127..20513
                     /locus_tag="PLI76_00435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI60525.1"
                     /translation="MTGAGDGELRFLVVEDDGIIAFDIVSRLKELGCGDVSVAATGAA
                     AIRQAERLQPHLIFMDIALKGGMDGIEAAEVIRDRFGIPIVYVTAHSDPAVRNRAMAT
                     SPAGYIVKPFTTADLVCAIRSALRTP"
     gene            complement(20488..21720)
                     /locus_tag="PLI76_00440"
     CDS             complement(20488..21720)
                     /locus_tag="PLI76_00440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HOI60526.1"
                     /translation="MPDEISELEEIKMLLRQEPLGLSILEISRRLNIGRNVTAKYLSM
                     LYAAGQVDLRRVAAAKLYTLAHRVPVSALLGLTSDLIVVLDRNLRVVLINDAFQALLR
                     LDRTELVGRQIGAFPPDRLPPPEIGERARVALRGQEVKCEIPWKADGRTVQFHATFVP
                     VVFEDGSPGVTAILEDVTREVEAAQDLEKALEEKEALINAIHHRLRNTLQVVSSILHL
                     QTTCLTDPAARRAIRATELQILSLALAHEDLYRSQYPDRVRMEEYLTRLTGTLLGSYG
                     VPPEKIAWKVRAPGIEMHLELAQFIGFLLTELVVNVIQHAFPDGMTGTMEVTIDRSRA
                     GRYTVTVRDTGVGISEEMHITATAAPGLPTVRLLVEEYLKGHVAVEWSGGTTVTVTFG
                     EEDMLVASPEATESEGQT"
     gene            22565..24967
                     /locus_tag="PLI76_00445"
     CDS             22565..24967
                     /locus_tag="PLI76_00445"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HOI60527.1"
                     /translation="MYMELLDSAAAIVSGTVAGGARVYVFLQRLGKAVGVGRVCLAGV
                     EYRPKAGEVRLRILCEWTAGGIAGKEEDGQSVAYQPLPPGWAKRLMAGFPVHGRFPAS
                     PGSVLMVPVFCGSELRGILEFRSRVARRGWRRSDVGTLKAVAGILGSVMAGSGAPHAV
                     GACSCAVVDDPTVPFCRLSPDGAIAYASDSFCALFYRARDDVIGISVSALFSPEIMKK
                     LPNVAGFMVTPEFRAPGADGGKHCCRSVCRTLLDDRGAVCGYRVFGCKSTEFKQQEVR
                     SVGTDDRMFTLLAEAVYDAVVVLDRSARITYWNRAAEDLFGYTADEVVGHDAGQLFSP
                     EHVHLLYLESFKHAVAAAAEGVVRLRFPDVSLVRKGGAVFPAALSIAAVLTRGAWFGI
                     GVVRDTTAEKIREQAILTEAERIGTLLRIAARLNGGVPFDAALDLLCEETARALRVSG
                     AVIYLYDLPGDLLAPARSFGVPEEVRKSLPPIPQALYEREVERQGSAFVIPDKRPWTD
                     VASGKKMPGNPLRPLASAAILHGRTLIGALNVMTLDTPRSLSPEELTLLKGIADLAAL
                     AIADIRFSAERAGHEHQLQALLDEKTALLKEVHHRVKNNMQVIASLLSLQGRLTENAV
                     ARACIRESENRVKSLALAHENFYRSESIAAVDAGDYLLRLVNDLLRLYAPPVHVSVDV
                     ETEKGLVLPGDVAIPLGLLLNELVSNSLRHGFAGRSEGAIAIRIVRATNRRLTIEYRD
                     DGAGIPGEFAARPPATLGLQLVRILAAQLEGTVTFRRGEPGTIVEITIGDGAGEGRMN
                     HG"
     gene            24960..25988
                     /locus_tag="PLI76_00450"
     CDS             24960..25988
                     /locus_tag="PLI76_00450"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOI60528.1"
                     /translation="MDSPSDTTEGHTVLLADPDPLTAATLSLRLQELGYAVVGAAAKS
                     DGALAAAAAFRPDVVIISADLPGPGSPRETALGIRDGLGIPVLVTAAHPVDTLSPDGE
                     EGLSPQEILVGLFGPDDLACAIEAVIARHRARHPCREWVEAFRQAAEIVPGFIMLLGT
                     DRHIRFVNLSGAAMAGRPRDALIGLDAVGTALPLSDRDGGDLRRRFDEEQEFHVAADC
                     IPIGRCRFSLRWTCQPVRDGRGCCAGWVCSGTGEGDGASPDASLQAEILLRFEKNMEQ
                     LAILNDRIRNPLQVIAAFAEFADDDIRDKILLQVKAINETVRQLDQGWLESATIREYL
                     RKHHRMAL"
     gene            complement(26020..26904)
                     /locus_tag="PLI76_00455"
     CDS             complement(26020..26904)
                     /locus_tag="PLI76_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOI60529.1"
                     /translation="MSPQRSAGRPAGGLISPAANTWCPGCGNFAIEHMMKSAIAELSE
                     EEGIPLHNFVLLGGIGCHGKLVDYLNVNGFYGIHGRSIPAATGMRLANPDLKIICHVG
                     DGDIYAEGLDHLIFAAKRNSDITVIVHDNRVYGLTTGQYTPTSPFGFRGRSTPGGISE
                     HPINPLELMLAAGATHIARGYTKKVRHLKDLFKEAILHRGFSFVDVLQICATYFNLTD
                     YYNEHTYEMQEGDVETGRFESALDKIREWDYDRDAPIALGTFYSVRRPIYEEKFQALT
                     AGRPDRRALVRKVIERSR"
     gene            complement(26901..28559)
                     /locus_tag="PLI76_00460"
     CDS             complement(26901..28559)
                     /locus_tag="PLI76_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOI60530.1"
                     /translation="MREYSVLIGGKAGEGINTAGLSIARLFSRLGYRTYMYFDYPSLI
                     RGGHNFAIIRASARPIGAHRTPVDVLLAFDQNSIENHRQRIHRGTTVIYDASQVTKGE
                     GYGLPIDTIVREEDAPPITGNSAMLGALARVAGIGRDVLGDVLRESVPAKHLEANLRV
                     AGRGYDGVESAFSVEPLDAPTLPIMTGNEAAGLGLVHGGLESYVAYPMTPTSNLLHFL
                     AGQAEDLSIKVLHPENEISVILMALGLAYAGEKAAVGTSGGGFCLMTEGLSFAGMAEI
                     PVTIVMGQRPGPSTGMPTYTAQTDLHFVLNAGQGEFPRFVVAPGDIEEAYTWSSTALM
                     LSWKYQVPSIILTDKTLAEGGYSFDIEKIALHPDLEPVLWDGAGEYRRYARTDDGVSP
                     LAFPGREGAVVRANSYAHDEVGFTTEEAGAVTALQEKRFAKGESLKAELAAYPTVKIY
                     GPRNAGATVVCWGSEKGACIEAAERFGARVVQPLVFAPFPARAWKEAMIGAGKVVCVE
                     NNATGQLARLLRQEGFDPGRPVLKYDGRPFAVDELEARLREVLA"
     gene            complement(28734..29480)
                     /locus_tag="PLI76_00465"
     CDS             complement(28734..29480)
                     /locus_tag="PLI76_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60531.1"
                     /translation="MPLTLRTWEVRLGIVLVASSAAIYTVKYLLLGDPENTYTYIFNA
                     LGFLPINVLLVTLILNQLLSIRAKRERLEKLNMVIGTFFSEAGTWLLAFISDRDSELP
                     ELREGLIVTEAWTPETFAGVCDRLQHHHARIAVDAADLQDLRRYLQGRRDFFLRLLEN
                     PALLEHESFTDLLRAVFHLTEELERRDDFAGLPASDVRHLTVDIERVYRLLAREWLAY
                     MEYLQKNYPYLFSLAMRSNPFDETASPVVR"
     gene            complement(29536..30669)
                     /locus_tag="PLI76_00470"
     CDS             complement(29536..30669)
                     /locus_tag="PLI76_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOI60532.1"
                     /translation="MHTDTSPTLLVTGGAGFIGSHIATELLQNGYHIRILDNLTPRMH
                     GPDRREPGHLDRRAEVVVGDIRDPHRLREALDGIDTVIHLAAVVGERSSMYRIEKYLS
                     VNTSGTAVLLEALLDHPIRQLVVASSSAIYGEGLYCSYNGTVYPKVRRFPGWAAKGDW
                     EPRSPDGEVIYPLPTPETKEASPSSVYAVSKYDQEEMCRMIGETYGIPTTILRLFPVY
                     GPHQGYANPYSGMLTEYAFRLLQGLPVRLFEDGYQQRDFVSVYDAARAFRLALESPRA
                     AGETLNIASGRPHTFWTVASRLMAITGRQDLSPDITGTGRVGDIRHCTADISRAREVL
                     GYEPEVSLEAGLLDLAAWVRAEIAREEKVPVPLKVPAPSRLRV"
     gene            complement(30834..32462)
                     /locus_tag="PLI76_00475"
     CDS             complement(30834..32462)
                     /locus_tag="PLI76_00475"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HOI60533.1"
                     /translation="MVEGNFYPSTGATMNLRRPNANEATGTSNRSRDVVPASGICTRC
                     IDGCKGACEIWLSSFRGREALYPGPFGEITAGADKNYPVDYSHLNIQGYAVGAKGLPE
                     GIEAGPDAAVFPAVDTRTEYGWSAKVPMRVPIFTGALGSTESARANWEQFAIGAAISG
                     ITLVCGENVCGVDPGLVLDHAGRVARSPEMDRRIEIYRRFHDRYGELLVQLNVEDTRL
                     GTAEYVSAKHNLETIELKWGQGAKCIGGEIRVGDIDQANRLKDRGYIVLPDPGLHEVQ
                     KAFEAGALREFERHSRVGFVTREGFLDEVDRLRDIGFKRVTLKTGAYSAVELAMAIRY
                     GAEAEIDLLTIDGAPGGTGMSPWPMMNEWGIPTFYIESLACRFCERLRERGIRVPDIA
                     IAGGFADEANVFKALAMGSPYVRAVCMGRALMIPGMVGKNIATWIEAGELPKTVSKYG
                     RTKEEIFVCYEELRERFPDRIGEIPLGAVGIYTYTQKFRTGLQQIMAGTRNFSLGTVA
                     RSDLMALTEEAEAISGIPYVMRAYSDAAEAILDS"
     gene            32574..33044
                     /locus_tag="PLI76_00480"
     CDS             32574..33044
                     /locus_tag="PLI76_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60534.1"
                     /translation="MNFRLLPALIVLLLAFSLGAACTGSGGEAGPSTPGTGTPAATAT
                     PTPTTEVSLVPGPTQTIPPGKEVTFQITPGYPSPFTHDLTITFNGGKGQDYVNGIALR
                     VTKSNGEVVEKTLQPRIGDAVTINEAEGDNRVEITVSLVTGGSYKVTDEVVKVP"
     gene            complement(33079..33774)
                     /locus_tag="PLI76_00485"
     CDS             complement(33079..33774)
                     /locus_tag="PLI76_00485"
                     /EC_number="1.1.1.302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone
                     5'-phosphate reductase"
                     /protein_id="HOI60535.1"
                     /translation="MRPFVFVNVAMSADGKISTRERRQVRISGSTDFARVDRIKAESD
                     AVMVGIGTVLADNPSLTVKSPEFRAGRRAAGRDEHPIRVVVDSAGRTPPDADILHKGA
                     GKRIVAVSRRADMERVAALGKHAVVVIAGDETVDLAALLAELYRQGVRRLMVEGGGTL
                     IWALFAQGLVDELQTFVGNIVIGGKDAPTPADGDGFTREVDFTRLRIIEIERLDDGLL
                     VRWSVEKESAARD"
     gene            34053..35771
                     /locus_tag="PLI76_00490"
     CDS             34053..35771
                     /locus_tag="PLI76_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HOI60536.1"
                     /translation="MIVILSFSAVSFMLTVIAPDYGIYPANSLIYCIPILLAALWYPG
                     QGFRVTALLVAGFALIRIYFLTLGFAIDPVMTGLNMLFFFWVFGATVLFSQDPYLTIS
                     RCRQVVENTRDARLLCDPETLRVLCVSRRCADILGYAPPELIGIPVEKFWPDESERAR
                     FIAEMKREGYIGNMEATFRTRNGDARAVLISCRSLADENLVLCTVVDIGGHAALVQEN
                     GRLMELIHQSNDIFFMQDVAGRILHFSWLRAPEYGISPEGLIGQGVDALLSADLAREH
                     AARLQDVVEGKKSTCYDISLELAGTRHVFSITMAPYYGVDGTLIGVAGSARDITEVWR
                     QRLACRQMALEIDQWKGLVATLSHELRTPLQPLIGHLQMIVEDPGYYGLSEEAKGPLA
                     TCLACARQEQAAVEKMVGFGLLAADSVELVIQSVPLRSLVDSIVSSGGYDREAEFCND
                     IPETASLRGDRDRLYEVIETLVSNAVKYNEPPKKVWASYTESNRNHYITVCDNGIGIS
                     ADAVESIFGPFSTGGTEQPNRERRQTDPGLSIANKYVRLHGGDITVRSVVGEGSTFTI
                     RIPKEV"
     gene            35791..36201
                     /locus_tag="PLI76_00495"
     CDS             35791..36201
                     /locus_tag="PLI76_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI60537.1"
                     /translation="MVVDDDLPTLEVMELLLKKIDREPLLVHNGWDALRMLKKEKPAL
                     IILDVMMSPIDGWQFLEELKRNEEFKAIPVLLFTAKHVWPEEYSRYAGDIVGVLEKPI
                     SLAELKTALERALPGSAASHRDNDTHARTQVRSG"
     gene            36316..37641
                     /locus_tag="PLI76_00500"
     CDS             36316..37641
                     /locus_tag="PLI76_00500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOI60538.1"
                     /translation="MIVTDLPCCPSLKEYRQIVGAEQFSAIEELAGRLSGIRMQEINS
                     TRQGGGVAEILASYVPFLNALGLETTWSVMEAKPAFFDVTKTLHNFLQGRDGFSPSMI
                     ETYWEAQRQNEGLIEDDADVVTIHDPQPLGLIEFLTGREREQKRLLWRCHVQLETVPT
                     GTAGSIGNILRRLVEKYHASIFSSFQYLPLWRVPGFIIPPFIDPLSEKNRDLPTAEVD
                     AVLAKYGIDAEKPIVTQVSRYDVFKDPVGVIEAFRKVRRKTPCQLVLVGGRACDDPEC
                     FLVLREVRETAEDDPDIHVLDLPPDSHREINALQRASDVIVQKSLKEGFGLTVTEGLW
                     KGKPVVAGNVGGIPLQVRDGWNGYLVSTVQETADRVLYLLRHPDAAGEMGIRGREFVR
                     EYYLLPRGVQDHLAVVDQVVNGKITARESVICYHPQVVTGRMAACAPRD"
     gene            37703..38146
                     /locus_tag="PLI76_00505"
     CDS             37703..38146
                     /locus_tag="PLI76_00505"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOI60539.1"
                     /translation="MKIAIASAGAGGLDDTVSPVFWASSSFTLIDAENQEILAVEIVV
                     APEGCAPGAAAGRFARDLAGRGVDAVIAGEFLPEETGVFSSAGIGMYRLQDMPIRDAA
                     KQVLNLAADTLRSPGRGAVPTDYVTRTYGGGVGLGSRRRTGGGPR"
     gene            complement(38153..38551)
                     /locus_tag="PLI76_00510"
     CDS             complement(38153..38551)
                     /locus_tag="PLI76_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOI60540.1"
                     /translation="MTGSEDEPSGTGRRETDSLPATARDYSILSAGFRVDILDRGDRV
                     IVIAELPGADEETIAIRLPNPQTLRITARRAGPAGEGAGDGTLSRLIRLPASVTAEGA
                     ATGYRNGVLEVSLKKVEGGSGPGGREIPIG"
     gene            38870..40114
                     /locus_tag="PLI76_00515"
     CDS             38870..40114
                     /locus_tag="PLI76_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60541.1"
                     /translation="MAQFKPFDMEGMPLEEQPMTWKDMSTAPYDKKAVDAYTRTRVIL
                     MNGIENNAVLMSHAIARMHPEPEVKKAMAMIRRIDSQHQVAINWLNPADQSVIETTLG
                     YEQVAVDLTANLAKNEPDPYVKQTLDFALLEDFDHLYRYSCLYDYIEGGDAEAIVQGK
                     TDVKPGRPTSIEHRHPDDSMRKHYDKDTADIKTKMNYVTIVAGEQQTMLYYRAHGSMY
                     PDDIARRLYAEIAEIEEQHVSQYELLGDPRETMLEKLALVQLNEAYLYYSYAQHESDP
                     RIKGIWETHMKMEIAHFNECARLIRKFEGRDIHDILKADVVEPLIVFESNKDYVDRVL
                     DAQLDLMPNNREYVRLRDLPDDWASFGYQAKVNAKGAPSEEIVSKAGRELAQRDQAEK
                     IRKVKQEMAREMEKGMAAVPAR"
     gene            40348..40587
                     /locus_tag="PLI76_00520"
     CDS             40348..40587
                     /locus_tag="PLI76_00520"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOI60542.1"
                     /translation="MPGFDGTGPRGMGPMTGRRMGRCALPPQTENSPAGEAGEGAPET
                     PVQQPQVYGLGRGGVPWGCGRGRGFGGGGRRRCRW"
     gene            complement(40744..41451)
                     /locus_tag="PLI76_00525"
     CDS             complement(40744..41451)
                     /locus_tag="PLI76_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOI60543.1"
                     /translation="MRIKFCGTASLTDMQCAVDAGCDAVGFIMGVTYRSADVVTPAEA
                     ADMIRHLPPFIEPVAVTHLQETTDLVRLARDSHCTTLQVQNTVEPSELSAIRGALPYV
                     KIVKAVHVTDESAIATAELYEPYADALILDSRTEERIGGTGIPHDWNISAKIVANSTI
                     PVILAGGLTPENVREAVRKVRPYGVDVHTGIKKDGLRNPEKTLAFAREAQNALPNSGN
                     KRSAGKIRSGNEFARIR"
     gene            complement(42009..>42258)
                     /locus_tag="PLI76_00530"
     CDS             complement(42009..>42258)
                     /locus_tag="PLI76_00530"
                     /inference="COORDINATES: protein motif:HMM:NF019884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HOI60544.1"
                     /translation="REEPSGKTSGKTSGKTSGKTSGLILEMIRQQPDITIPEMAEALG
                     RSTRAIEMQLAKLKKSGKVQRIGPAKGGRWEIVGGDTP"
BASE COUNT         7284 a        13478 c        13686 g         7810 t
ORIGIN      
        1 ggcaaagaaa gtgatcgcgt tctaccggga gaacgggcag gcccccgaac gcctgggagc
       61 gacgatcgac cgggtcggct tcgacaaatt caagaacgct gtcctctaaa cccctggaaa
      121 aggttttacg ggagatcgat gcatccgcat gccggcggac ggcactaact cccaccccat
      181 ttatctctcc tctgttctca cgaagtggtg gcgagccggg tttatctccg gcgcagcccg
      241 ccggggatca tcctgcatac cacggcatac acttcccgag accaccccgg gagaaaaaaa
      301 gaatatcagc cgatatagtt caattcttcc cggccccggg cttcctcggt ttcccggata
      361 tttgctatga tctgctccat ggcctcggca cgctgctgca gggtggtgtg gtcgatatcg
      421 atcccgatca tggcggagag gacggtgagg agactgtcgg cgctcctcgg atcgacgatg
      481 taaccgctcg tctcgcccat caggcagatg ccctcgatgc cccgcgcctt cccgaggccg
      541 agcaggaggc ccgatgcccc catgatcatg ccgccgctcc cggcgttctc aaacgatccc
      601 ccggccgcct ccacctccgg ccggagacgc tccatattga tcgcggagag cacccgcggg
      661 tgctcgacca ggtggccgac gccgtatccc ccgagcgtgt agatcctccg gacgccaaga
      721 gcctcggcga ggtccaggta ccgctcggcc aggacgtagt gcccctcggc cgaggtgctc
      781 tggaagtcgc ccacgaggaa gaggatcgcc accccatcct tctcgcagcg atagatctcg
      841 ttgttgacca ggtgggtgac gccctgttcg tcgacgacca cctgcggcgg gaagtggacc
      901 gaggatatct ccgctatctt ctcgccgccg agttcatcga tgaggtggtc ggctacaagt
      961 ttcccgacgt gcccgatgcc gggcaggccc tcgaccagga ccggagcgcc gccggcgtca
     1021 tcgcggaaga aggtcacgac gacgtcttcc atcggcgcat caccctcctg tagtccccat
     1081 gcctatcctc gtgagaaaag cgtgccggat gagccggtcg cgtcgaccgg ccgcatatcg
     1141 gacagacccg cgcaagcgta tacctcaggt cgtccggaca gcggcgaatg cgattgctca
     1201 tggcgcgtta tccggccttc tgcttccgga cgaacttgcc ggaaccgccc gcccgctcga
     1261 ccacgccgac ggcggcgctc gccgccttct cgatcgcccg ctcggccgcc ttgtaatcgg
     1321 gagcggtcac ttttatccgg tatttcggtg ctccgatgta gatcaggtcg atatccacat
     1381 cctcgatctt cggctgcgcg ctcctgagcg cacggcggat aacgttgacg ccgtcgggcc
     1441 gcggcgacgt caggacgaga tgccccgtga tggtcacctt cgagaccttc acgttctcgt
     1501 ttgcgatcgt gatgagcgcc tctttgaccg gttcgtcgag gcccagcctc tcggccgcct
     1561 cgccgccggt ggtgacgata tcctcgaggg ccggatagag aaggccgtat tcgcggtata
     1621 tcgactcttc gatgaccttt cggtccacac ccgcggactc ggaggcaaac cctatccact
     1681 tctcggcctt ctgctcgttc ttccactcgt ggatcttctc gcggcgctgg tgctcgttga
     1741 catcctttaa cgagagatcg atgtggccgc ggccggggtc gacacccagg actttgcaga
     1801 cgaccttctg tccttcccgt atgtagtccc ggatatactt tatccagccc cgcgctatct
     1861 cggatatcgg tatgagccct cttcgcccgt cgtactcgtc cagggtcacg aacgccgcaa
     1921 aatccttgac atccgcgacc gtgcagacaa cgagctctcc ttcctcgggc cactctctct
     1981 cgtgcatgga ataatcactc aagtactgcc agtatatcag cctttatatc tgcctttccg
     2041 ccgtggggct cggcgaggat acgcccgcag acgctgcacg ccacgacggt gcttgctctc
     2101 tcaaagacta tctgttcgtt ctcacagtcg gggcacttca cccggaggaa cgtgctcctg
     2161 ttctctctat tcaaccggac cattattaat ctcactccgt aagctcgaat ttaccgatcc
     2221 ggaatcccgg ccggaggtgc gccttcccgc agacggtgca gcgatatttc acgttgatct
     2281 tcttcgtcgg cttgtcgccg ccgggcacct tgctgaactt gcccatgttg cccaccatac
     2341 ttctacgatc tttctggcgg tcgatccagt tgaagtggcg tactttgccc ctcttcacct
     2401 tcacgacctc atgagtctgg tgattccggc agaatggaca gtacgtcttg aattttgatg
     2461 gcattttcat gatagcagcc tctattcaat aggttcgagt acctatatta cttgttaagc
     2521 ctaatattta aggctatgtc gtgttcgcag agcacgtctg cattattctc cggcagggtg
     2581 acgatatccc cctgcgttaa gacgtagacc ctgccgtcca ctcccatgaa cgattccaca
     2641 tcggagcgga tatgcacgag ggcatacgga gacgagagcg ggcggggaat tgtcggggcc
     2701 ggcctccctg ctccggcggc gggagggata tccggctcct cgatcgcgtc cacggccgcc
     2761 gcaccgccgc cctggaatga accttcctcc ccgcggatga gcgtctcgcg gcaaccctcg
     2821 acggcgtcga tgatctgccc gtacatcgcc cgttctgcgg ggagcatctt ccttgcctcc
     2881 cccctgtcga agtactgccc ctcgatctgc tggaaagcaa gatcgagtat ccgccgggtc
     2941 cggatggcaa atatttcctg cacggtctcg gtgatgctcc cgatctcctc gatgagcagg
     3001 cttcccgact ggctctcgag ggggttaggg agaccgtagt agtcggcctt gagtttctcg
     3061 acgtatgccc gggcaccctc gtaaagagcg ggctcgatat cggagagcct gcccgagtgg
     3121 tgcatctcaa ggagcgtctg gcgcagcctc tccaaggggt cggcggccat ggttcaggcg
     3181 cccccggcaa tctccgccat gctccggcag cagaggaaga ccgcaagcgc ttcgggaacg
     3241 cttgcaggac cggttttgag cgtatacgtg ttcccgagca gggatgtggc gacgttcatc
     3301 tgcagatcca cccgcacctc gcggtccccg aataccaccg catccacgag cgggtcgaac
     3361 tccgcgaggt cccgggaatc gagcggcacc acccgcatgc cgctgccgcc gtgcagcgac
     3421 ccgggcgccc ggatcagccg cttgatatcg gtcgtgaccg gttcgtccgc acgcgccccg
     3481 gcgccccgga cccgctcctc gaacgcaggg gtgcccatga tcgcccggac gatccggttc
     3541 tcccggaggg tgccggggtc cttctcccgg agggcgccaa ggcctgcaag aaactccgta
     3601 gccgcccgtt tcccgatacc cgccggtccc gtgaggtatg ccgccgcctc cgccgcatcg
     3661 agcccctcga gccagaggag gtggtcgcgg agggcctcga tgtagcgcca ccgccacccg
     3721 gccggggttc tcccgggagc aagcatcacc cccgggtcaa gcccgatgcc gcagacgtaa
     3781 tcgacgacct cgcgccgctc cgggctctcc catcccctga cggcgatatc ggtgacgtgg
     3841 atatggtagc cgcgcccccc ggagaaggcg atccggatgt ggttacgcgc aaaaccgagc
     3901 tcgtcggtca gcatcccgac cagtttcacg gtctcctcct tgacccgggc aagcatcatc
     3961 gcgtaggggc cgcggacgat ctggtcggcg tcgaggtcga agatcaggtc ggcgccggcc
     4021 cagtgcttgt cgttcatcgt cggcgccgac ggcgtctggt agtaggccgt cgaatagtag
     4081 gcgtgggcgg ggatcaggct cctgacgtaa gcggtaagtt ccagcgggtc ggcaaacgcc
     4141 atatgccgcc gcatccggac ggcggacgcg gcatcgaaga agacaaaacc ccattcgcgc
     4201 tgctcgagag aggatggagc ggcgagggtc tgcctctggt agtactcggc aaacctctgc
     4261 ttcacaaact cgatcgtcgc aggcttcatg agatcgtctt gatcatatat ggccccactc
     4321 gttcatatcc ctgtttcctg tagtaggggc gcaccccgac accgctcatc accgcgagcc
     4381 gcccgtagcc gtggccggag gcctcctcct cggccgcggc aaggagccgc acgccgtaac
     4441 tgcggtgctg ccactcgtcg gccgctgccg gcgtgccgag ggggaccacg ctcccgtaga
     4501 cgtggagctc gcgcaggagc gccgcgccgg caagctcgtc gcggaatggc cggtgcggga
     4561 accggagccg ggcaaacccg acgagggcat cctcagagcc ggcctggatg aaccgttcct
     4621 ccccgccgca ggacgggtag gcgacggtcg atagcgccgt ctcttccggc gcaaccgacc
     4681 tcccgacctc gcggcaccgg atgcaccggc atctgagccc ctgcgagtgg agccgcgccg
     4741 ccgcgagctg ccggaagttg ctgtgccgcg aacccgcgac gatcaacttt gccgggatat
     4801 cccgctggat ccgctggagg cggacgtact ccgggaggag cgacttcgcg taggccacga
     4861 ggtcgaccag ctcctcctcg gtgtaggggc ggtagtcgcc ccgctcccag agtgcctcga
     4921 tctcggaggc gggcgtcacc agggtcgggt agatcttcag aaagtccggc cggaaccgcg
     4981 gatcggcaaa gagttcgcga aacatcctcc ggtcgtcctc catgctcgcc ccggggaggt
     5041 tcggcatcac gtggaacccc accttcaggc cggcgtcgcg caggaggcag tttgccgcga
     5101 ccgagtccgc gaccaggtgg ccccggcggt tgtagtcgag gatccggtcg tcgatctgct
     5161 ggacgccgag ctccaccttg gtcacgccca tcgcaagcat ccggtcgatg tgttccgccc
     5221 ggcaccagtc cggcctcgtc tcgaacgtcg tcgcgatgca ccggaccccg gcgcgctcgt
     5281 ttgcggcgaa tatagcgtcg aggtcgggtt tctccggcgg gacgccggcc cgggggtagt
     5341 cgttcatcgc ctgcacggcg gccccgacga accactcctg gtactcgacc gaccgggcgg
     5401 tcatcgtccc gcccatgacg atcagctcgg ccttgtcgac atggtggccg agcgcctcga
     5461 actgcccgag acgggcctgc acctggcggt aagggtcgaa cgcatgttcg cgggcccgga
     5521 gcgccgccgg ctcctcgccg gtgtagctct ggggagaccc gaacgggtgt tccggcccgc
     5581 cggggcaggg gaggcacttc ccgtgcgggc aggggtgcgg cgacgtcatc accgcgacgg
     5641 gcgccacccc cgagagcgtc cgggtgggtt tgacgaggag gagcggccgg aggcgttcgc
     5701 gctcatccgg cgtggctgcg gcgaggactg cggagttcct gggcatgttc gcaccgtgct
     5761 cccggcagac ctcgagcttg atcctctgga tgtcggcagg gtcgggcccg gcagaaagga
     5821 tgcgggagat gatctcccgg agggtatcag tatcgttcat cgcttaatgt tcggttgcaa
     5881 tgcgccccga tggaaaatca gcgtgataat cgatgtaagg attatggttg gcccggatat
     5941 gctggacgat ctcgtttccg agcgtcagaa tggctttttt atgcgttact ttattcttat
     6001 ggatatggac gggtgagatg tggagagcat tatactggtc ggtcgagacg tcctcccccg
     6061 tagtgctttc ataatacttt cgtacgtgga ctaacagcat gtgtagatgg agcagctctt
     6121 ctttttgcac aatatcacct ttcctcgaag aatactactg cccgcgggta gatataattt
     6181 gttggtgatc tttttatatg cacaaggtgc gggcagtcgg agcgttcatc gggcttgcaa
     6241 tcggcgatgc actgggcgcg cccctggaag gctttccgcc gcctccgttc accgtaacgg
     6301 agatggaagg gggcggaatg caccacaccc ttcccgggca gtacactgac gataccctgc
     6361 aggcgtcagc cctcgcggag accctcgtcc ggtgcggggg tctggatccg ggcgacttct
     6421 cccgtcgcct gatttcggct taccgggctc gtccggagtt cttcgggccg acatcccggg
     6481 ccgtcctcga cctcgtcgag gggggcgtcc gcccggagga tgccgcgagg atggtgcacg
     6541 atgcgcgggg agggagccgg agcaacggga gtgtgatgcg ggggataccg gtcggcatct
     6601 tctatccgcc ggatgtggtg cgggaggcga gcctcgcggc atcacgggtc acgcactttg
     6661 atgccgcagc cggcgaggcc tcggcgttcg tcaaccggat ggtcagcggg atgtgccggg
     6721 gagaagaagc gagcgcggcg ttcggatacg ccctcgccgc ctgccgcgac ccggaactcc
     6781 acggcctcct ccgggactgg cgagcgcacc cgcgcgaacc ctcgctcgat gccgtcctct
     6841 gcacccactg cgccgtcagc gtcttcatgg atgcaccgac cttccgcgat gcggtgatca
     6901 cggcggtcaa cctcgggggc gacgccgata ccgtcggcgc gatcgccggc ggccttgccg
     6961 gagcgtgctg ggggcttcac gcgataccgg gctcctggct ctccgcgctc caggaccgcg
     7021 agatgctcct cgaccttgcc cggcggctct cacaggcagg caggccgtag tgcacgatat
     7081 cgccccggga agttccggtg catgccggga tgggtactct ggcttctcgc gagaggccgt
     7141 atcgccatcc gcaccggtca cctcgcgcga agacttgccc gggtgcctgc aactgtagcg
     7201 tcagatgggc cgcagttcca cgagtttcag gggccggccc ctctcgcaga cgtcgggggc
     7261 gttgccgagg acctccccga cgatatactt ctccccctcg atgatgccgt cggggcggca
     7321 gagccggtag ctccggcacg tggtccgcgg gcaggagaac tcgtaactga ttttggagtt
     7381 cgcgatcgcc atatcggcgc ctatcagcgc gacgatggga gcctccagca cgtcgaccgc
     7441 gaccaccgcc tcgtggtgga cgggacagtc ctgccgggtg ttggagcgga tggcagcgac
     7501 ccggtacttc ttcccgggct gcaggttgtg gcaggtctta cgcaccttgc agctctcgca
     7561 ctcggtgcac acgttgcctt cgtagacgaa atcgagcccc ggttttgcaa gcgcggcgcc
     7621 gatcagggtc acttttgtct ttggtttcac catactttcg ctcactcccg gtacagcatc
     7681 ttcacgaggt cccgcgccga tgccgcggtc agccccatat cgaggatcgt gaaccgctcc
     7741 ggccggatgg tccgggcggc aagcagcgcc tccacggccg tctcatcgtc gatcccgatc
     7801 tcggccgggg tggtcggcgc ccccaccttc ttcaacgagt cgcggatccc ctcccagtcc
     7861 ccgccgtgga gatacatcgt tatgatggcg ccgataccgc acttctcccc gtggaggccc
     7921 ttccccggcg cgagccggtc gagggcgtgg gagaacttgt gctcgccgcc gctggccggc
     7981 cgtgacgagc ccgcgatgct catcgcgacc cccgaagaga ccagcgcttt cgtcacgatc
     8041 caggcgctct cttcggagtg cggtttgatg agatcggcgt tcttgaagag gatctcggcg
     8101 gtcatccgtg agagcgtcag cgcatactcc gagagcgctt cgcccctgag gcggtgggag
     8161 agttcccagt cgaggatcgc ggtgtggttt gccatgatgt ccgcacaccc tgacgcaagc
     8221 agccggtggg gtgccgacgc gatgatggcg gtatcggcga cgacggcgat cggcggttct
     8281 gcggcaagcg agacgttccc gtcggcggtc gggaccgagg ccctcgatga ggcgatgccg
     8341 tcgtgcgatg cggcggtggg gacgctgatg aagtggcggt cggtgttgta ggaggcgatc
     8401 tttgcggtat cgatgaccct gccgccgccg acgcctatga cgaaaccggc ctttgccgcc
     8461 gacgcctcgg ccctcttgat cgtctcgacg ggattgtttg ggtcggctgc acacgtcgcg
     8521 acgtcgtagg agccggcaag cagcgcctcg acccgtctcc cggccagatc ccttgtccgg
     8581 tctcccgata tgatgagcac ggagtcgccg agagcaaggt cctcgcagac ggtcgggagc
     8641 tgctcgatga cgtcgtggcc gatgacgacg tcgcggggga gctgcatcca tttggactta
     8701 tcgaagacct tggttctgag taggtttatg ccgtctgcgc tcattcagat cagtaatgat
     8761 tagggagttc ctctacaaat actacatcga tcccatccgt tacggtcagc cgtatacgct
     8821 cgtcgatacg ctgacctacg ccctgatcct catcgcagcg gtctatatcg tctaccgggg
     8881 gctccggcgg tataagattg ccgtcgacga cgaactggtg cttgcaacca tgcccttcgt
     8941 cgtcctcggc gggcttctcc gggtcgtcga ggatacgggc atgatcgcgt cggacttccg
     9001 gtttctcctg atcacgcccc tgatcttctt tacgatcttt gccgtcgcgg cggtctccct
     9061 ctttcttggg aaactcgcgg agaacgcggg cttcgtcgcc cgctacagcc gggtctacgg
     9121 gggagcgggc atcgccgcct gcatcgtggc cggtgcggcc ctcgtctggt tcggcctcac
     9181 cgagacgaag atcgcgctcg acgtccttgc cgccatactc gcgctcgcgt cggtctcgtc
     9241 gctcgccctc tgggccctcc tcgcctacgg cgcaaaatgg gactacgcct cgaacatatt
     9301 ttacaaactc ctcatattcg gccacatgct ggacgcaagc gcgacgagtt acggcatcga
     9361 gatccatccc atccattacg tcgagcagca cgtcgtgggc tcgggcctga tcgaggcgac
     9421 cggcaccgcg ttctctatgt tcctcctcaa gatcgtggtg ctcatacctg ccatctacat
     9481 cctcgaacag taccggcggg agggaaatcc cggtctctgg cacctcatac tgctcgccat
     9541 gatcaccgtc ggcatggcgc cgggggtacg ggatatggta cggatggtgc tctatgtttg
     9601 aacgcccgat cgtcggggcc acgatcgttg cggccgccct ctttctcgtc tcggtcggcc
     9661 tcggcattgt tgccgtgatc caggacccgt cgaccggcgc acaggtcatg accctctttc
     9721 gcgatcaggt cgtagatcag ctcctctccg actccccggc ggtcctcgcg ggcaagatct
     9781 tcgtaaacaa cctcggcacc tgcctcctcc tcttcctcgg cggcgcatcc ctcggcatcg
     9841 tcaccctgat tgtccttacg gtgaacgggc tccttatcgg tgcggtggcg gaactcgtgc
     9901 ggcagcagca gggcctcctc ttcatcgcgg cggcgctcgc cccgcacggc atattcgaga
     9961 taccctcttt cctcatcgcc gggggcctcg ggttcctcct cgggcaggaa ctcatcgccg
    10021 agtggcacgg gagaggcgat gccgccgccg gcgccctgcc cctggcccgc ctctttctca
    10081 gggtagtcgt cccgctcctc gcggttgccg cgattgtgga ggcatttatt acgccggcag
    10141 tcctaagtgt tatcacataa agcaggcaga tcgacgcttt ttgaggtacg agacggatgg
    10201 aagaagatac tggcgcactc ccccggaaag acgatttttc cgagtggtat aacgaggttc
    10261 tctggcgagc cgagatcatg gatgtccgct acccggtcaa ggggctctac gtctggtacc
    10321 cccacgggtt tgccatcagg aagcgtgcat acggcatcct ccgggacctg atggaccgcg
    10381 accacgcgga gacgatgttc cccctcctca tccccgagac cgagttcatg aaggaggcca
    10441 cgcacatcaa ggggttcgag gacgaggtct actgggtcac ccacggcggc aggaacgagc
    10501 tcgatgtgcc gctcgccctc cggcccacga gcgagaccgc catctaccct atgtactcgc
    10561 tctggatccg gtctcacacg gacctcccgc tcaaactcta ccagatcgtc aacacgttcc
    10621 gctacgagac gaaacacacc cgccccctca tccgcctccg tgagatcacg tcgtttaaag
    10681 aggcgcacac cgtgcacgcg acgcgcgagg aggcggaagc gcaggtcgag actgcgctcg
    10741 gcctctaccg ggagttctac gagagcctcc gggtgccggt gatcatatcc cgccgcccgg
    10801 cctgggacaa gttccccggc gccgactata ccatcgcggt cgacaccatc atgccggacg
    10861 gaaagaccct ccagataggg acggcgcaca tgctcggcga ccacttctcc cggacctacg
    10921 agatcaccta cgaggacgcg aacggggagc ggcagtatgc cagccagacc tgctacggca
    10981 tatccgagcg gtcgattgcg gccgttatca gcgtccacgg cgacgataag gggctcgtgc
    11041 tgccgccgga ggtcgcgccg atcgagatcg tcgtcgtgcc gatcatcgtc gggaagcggc
    11101 gcgacgaagt gcttcttgcg gcaagagcgc tcgaagagga gctccgaagc gccgggttcg
    11161 tcgtgaagct ggacgaccgc gacctgcgcc cgggtgcgaa gtactaccac tgggagatgc
    11221 gcggcgtccc gctgcgtctc gagatcgggc cgcgcgacat cgacgcgaac acggtcgtcg
    11281 ccgtgacccg cgacggccat aagatcacgc tcgaccggcg gggcgtcgtc gaaggtgtct
    11341 tctcggccct tgcggcgttc aggaaagggc tggagcagac ggcgcggcag gcgatgatcg
    11401 accggatcac cgtggcggcg acactggaag agaccgttct tgcggtgaag aagggggttg
    11461 cggtcgtcca ctggtgcggg tcaaaggagt gtgcggaaga gatcgaggcg gcggtggatg
    11521 caagcatcct cgggtccgac atccggtcgg acctgatcac cgtctctcgc ggtccgtgca
    11581 tcgcctgcgg cggagacggc acgtccaccc tggttgcccg gacctactga tcagcaggac
    11641 gggcaggtat actcccgggg gtgctcgggg gacgatatgt ctcccgatat agccgctttt
    11701 cccccggaga agaatatcct gccacatttc gagcatctct tcgggagcag acgctcccag
    11761 cggctcatcg gaacttccag cgtgaactgc atcttctccg catcggcggg gttgcactgg
    11821 gcgagctcct cataccggga gaggacctgc atgacctgga gcgggtttgc tccctcgttg
    11881 cagaatcgcc tgaatataaa aaattgaagg atcatccacc caaggtcgga gcgctcgagg
    11941 gtctcctcgt actcgctgca gaccccttcg tcttcatcga gagagtaccc gcagagggga
    12001 cagcgcccgc ccacgagttc gtccaggaag acctcttcat ggcagccggg acaggtggtg
    12061 tggggggtat gcattctgga agtcatggtt gtgctggcct catacttttt actcttccag
    12121 cggcacggga gagagccgcg tgccgtttga aagtattcca cgcattatgc gcggtctcta
    12181 caattgccct gaggttttac tacgtattgc gctcgtggtg ctcttatacg tgtcgaatgc
    12241 ggttatacgg atccactctg gcggcgcgtg ccggccgctg cggcgcatcg ctcgcccacc
    12301 cgctctccgg ccggagccgc acccttctgc cgccgcccgc tcccggcgat ccgaatcccg
    12361 cgaaaaggca atattttccg gatctgggct tttttggggc cgcccggagt acatttttat
    12421 agattggcgg ggcactctgc gcgcatggca tccccggcgg catctgaagt ctatatcatc
    12481 gacgcatcgg atcgtgacca cgccatcgag gcgctctggc gcgaggtcgg ctttccctcc
    12541 ctcgatggaa aggatatcgc ggtcaaggcg aacttcaaca gcgacgaccc gttccccgcg
    12601 acgacgcatc ccgataccct gggggccatc ttcggccagc tcctcgatgc cggcgcccgg
    12661 acgatccgcc tcggggaacg gagcggtatg ggggagaccc gtgcggtctt gaggaaccgg
    12721 ggggccgtcg acgtcgctgc ccgggcaggg gcggatgtgg tggcgctcga cgatctcccc
    12781 cgtgaagggt gggaggcggt tccgggagac ggccttcact ggagagaggg gttcctgatc
    12841 gcccggctct tttgggatgc cgatgtggtg gtccagacct gctgcctgaa gactcaccgc
    12901 ttcggcgggc acgtctcgct ctcgctgaag aaccctgtcg gggccgtggc ggagaagaac
    12961 cccgggggcg gctacaacta catgatggag ctgcatgcat cgccgcacca gcgccggatg
    13021 atcgccgagg tcaaccggtt cttcccctgc gctctctcgg tcgtggatgc ggcggagggg
    13081 ttctcgaccg ggggccccga gcgcggccac cgtatcgctc ccggcgtcat actcgcgagc
    13141 accgaccggg tcgccctcga cgccgccggt gtcgccctcc tccgcctgta cggttcgacg
    13201 ccggatgtga tgcggggccg gatctttgca atggaccaga tcgcccgggc ggcggagctc
    13261 ggcgtcggtg tccggtcggc gcaggacctc aggctcgtcg cgctcgatgc ggagagcaag
    13321 gagaccgcct tcgacatgcg gcggatcctg gacggggccg gctaaagacc gagcaggatg
    13381 cgcaggccga tgccgatcag gatcgccccg ccgaggacct cgaccttctg cccgaaggcc
    13441 cggccaagcg tactccctat caggacgccg gcagcggaga gggcaaatgt cacgatgccg
    13501 ataacgaccg caggccagag gaccggcgtc cccagaacgg caaacgtgac gccgacggcg
    13561 aacgcatcga tgctggtcgc gaccgcgagc aggagaaggg tcgctatgcc cacggtggcg
    13621 agctgcacgg cctcgccgtc tccccgcacc gctccggcga tcatcctgcc gccgaccagg
    13681 gcaagcagca ggaaggctat ccagcggtcg taggtgccga cgaaggaggc gaggcctgcc
    13741 ccgccgagcc acccgagcac cggcatcccc gcctggaatc cgccaaagag cgccccgacg
    13801 acgagggcgt acctgaaccg ggcctcccgc acggtggtgc cgccgctcgc ggcaacggca
    13861 aatgcatcca tggcaaggcc gacggcgatg aggagggttg tcgcgaactc catatctcct
    13921 gtacatgaag gtgcgatgcc ggataaccgt atggcccggc ggttgttata agacgcggga
    13981 gtgccctacg atacggtgat acagatgagc acacagaaga tcggcctcat ccagacggcg
    14041 gtgggggacg acctcgaccg caacctggcc cacgcccttg ccctgacgaa acaggcgata
    14101 gcggcgggtg cccgggtcat ctgcctgcag gagctctacc ggacccccta cttcccgcag
    14161 tacgagggaa gggacgcatc tttctacgcc gagaccatac cgggagcctc gaccgatgcg
    14221 ttctcggcgc ttgcccggga gcacggcgtc gtgatcgtcg tgccgctcta cgagcggacg
    14281 gcggacggcg agttctacaa cacggcggtc gtcgtcgacg cggacgggca cctcctcccc
    14341 ccctaccgga aggtgcacat cccctacgac cccctcttct acgagaagac ctacttccgg
    14401 ccgggggacc gttaccgggt ctacgatacc cgctacggcc ggattgccgt tctcatctgt
    14461 tacgaccaat ggttcccgga ggccgcgagg gctgttgctc tgcagggcgc agagatcgta
    14521 ttctacccga ccgccatcgg ccggattgcg ggcgaggagg agccgcccga gggcgactgg
    14581 cgcgatgcct gggagacggt gcagcggggc cacgcgatag caaacagcgt ccacgtcgcc
    14641 gccgtcaacc gcgtgggcag cgagggcgat atccggttct tcgggagctc gttcgtcgcc
    14701 gacgccttcg gaaacgtcct tgcccgggcg ggggagaccg ccgaggagat cctcgtcgtt
    14761 gaggtcgacc ttgccggaaa cgaggccgtc cgggaggggt gggggttctt ccggaaccgg
    14821 cggccggaga cctacggggc gatcacccgg cggttcctgc cggcaaagac accgcaggcg
    14881 ctcggctacc gcatgccggc ggagtgggag ccgcacgatg ccgtctggct ctcctggccc
    14941 cacgaccgcg agacgttctc cgaccttgca gcggtggaaa gagcttacgt cgcgatcatc
    15001 gcggcgctcc gcgggtccga gaccgtcgac ctgctcgtca cgagcgatat gatgcgcacc
    15061 cgaatcaagg cgatgctcga ggaggagggc gccgataccg ggcacgtcag gttccacatc
    15121 gccgaatacg ccgacgtctg gttccgcgac tacggcccga cgttcgtggt cgaccgaagg
    15181 accggtggtc ttgcgatggt gaactggacg ttcaacgcct ggggggagaa gtaccgggag
    15241 ttgatgggcg atacccggat accgctcatc atgaaccggg agatgaacct ccccctcttt
    15301 acgcccggca tcgtcatgga ggggggttcg atcgatccga acgggcgcgg cacggtgctc
    15361 acgacggagg cctgcctctt gaacccgaac cggaacccgc acctctcccg cgaggagata
    15421 gaggcctacc tggaggctta cctcgacgcc ggccacgtca tatggctcaa gcagggtatc
    15481 gccggcgacg ataccgacgg ccacatcgac gacgtcgccc ggttcgtggg cccgacgacg
    15541 gtcctctgcg ccctcgagga gaacgaggac gacgagaact acgcggtcct gcaggagaac
    15601 tacgctatcc tccgggcgtc gaccgaccag gacggcaacc ccctgacggt cataccgctc
    15661 ccgatgccgg gggcggtcga cggcgaggac cgcctgccgg caagttatgc gaacttctac
    15721 atcgggaaca ccgtcgtgct ggtgccggtc tttggccacc cgaacgacga gactgctctt
    15781 gcccggattc ggcaggcctt cccggaccgc gaggtcgtcg gtatcgactg cacggccatg
    15841 gtcgcgggat tcggcgccat ccactgcatc agccagcagc agccgtcggt gcagcgcccc
    15901 gaaagcaagt gaacgcgccg cgttcaccag ccgttgtagt ggacccggtc ctcgctgtag
    15961 cggtcgaccg gcggagggcg tgcggccggg tggccgacgg tgacgagggc gaaggggatg
    16021 acggtgcccg ggagcgaaaa gaggtttgca aacccgagca tccggtcggc gtccgggtag
    16081 acggcggtcc agaccgcccc gagaccgagc gcatgggcgg cgaggaggag gttttgtgcc
    16141 gccgcggcag agccctgtac ctggaggccg gggctctccg gtggggcgag gtcggtagag
    16201 ataagaatcg cgagcggcgg aggaggagag actctcgcgt aggggccgat catcggtatg
    16261 cgggcgagga tccgggggtc gtcgatgacg acgaagtgcc agggcggtct gtcgccggca
    16321 aacggagcct gcatcgcggc cgcgaggagt cttcgcacgg tctcccccgg gaccgggtct
    16381 gccgtgtact cccgcacgct ccgccgggtc atgatcgctt cgagggcatc catcaggcta
    16441 ccctcgcatc aatcattccc gggaacgggc cttcccaccg gcatcaggta aagcggtgtc
    16501 gcgttctttg ggagcatgag gatctcttcg acccggtcat cgtcgaacgc cccgatggcg
    16561 accgtgccga ggttgagcgc cgccgcctgg aggtagacgt tctctgcggc gtgcccggcc
    16621 tccatggcga cgtagcgcgt accgcgctcg ccgtacttcg cggtcgtcct ctccgggaca
    16681 gcggcgatga cgagggttgc cggggcgtcg cctacggagg cctggtcgac ggccgccgcc
    16741 tggaggtcgg cccgccggtc cccgccgccg atgcggacga gcaggtgtcc atcgggccgg
    16801 tagcggtaga cccccgcctc gaggtcgacg acgctgcctg caacgacgta gacctccagg
    16861 gggtagaggg cgccggcgga gggagttgtc cggtaacccc ggtcgttcgt gatcccctgc
    16921 gccgcccaga gtacctgccc gacgtcggca agcatgagcg gcacttcggc gtagatccgg
    16981 gcggaacgcc gcttccaaag cgcctcctcg accgagacgc cgccctcgga gcggggggcc
    17041 gggaggatga cggcatcgtc cggcggtgcc ggagtgctgt tcttgcccgg caagaagaga
    17101 ttgccggcgc atcctgcggc aagcaccgcc gcgagcagca ggccgatgga gaggatctct
    17161 ggatccatgg cacgcggggg gtgcccgggc agatgaaggt atcggagcgc ctccgcaccc
    17221 cgggccgcag gaagcctgcc ataggcgccg gagctccggg atacgccggc gcagccgact
    17281 atctcttctt cctccccctc gcggacgaga aggggaactt caccaggtcg cggagacctc
    17341 ccgccgggga gaagttccgc atgtcccgcc ggacccctgc gatgagtgcg tcgacgacct
    17401 cctgctcctt tgcctccccc tcctcctgca cagcggccag gcggtgcagg gagtagagac
    17461 cgaggatgaa ggcaaagagg aagaagaact cccactgccg caggtcaagg gtgacaaaga
    17521 cgagttcccg caccgggctc ttccagatca acgtccatag gagttcacgc tcggcaaaga
    17581 agtccacgaa gaggccgccg agtatcgggg caatcccggc ggcgaccgag ttcgtgaagg
    17641 tgctcgccgc gaggtaaccg gtcgcctggc ctctcggcgc catcttcagg cttatgtttc
    17701 cggacgcaag cgataccccc gcaagcgata cccccatcag tatgtggatg aggacgagca
    17761 gcgggtaggt caggatgtag acgtcgggga gcgtgacgaa catccacgcg aagatcgcga
    17821 gcatgaagag cggaccgctc acggcgagga ccgatttgtt ggagtagcgg tcggagatcc
    17881 ggccccagga ccggtagaag acgatgttca tgatctggct cccgacgccg agggcgacga
    17941 cgaccgatat atcgaggcct atcgtctgca gcatgtagac cgtgaagaac ggcgcggcaa
    18001 ggttgacggc gaagttccaa gaaccgagga atatgatgag gttcttgaag ttgacgtccg
    18061 taaacggctt tttgatggtg gcaagcaggc cgtcctcctc gtcggcggca accatccggg
    18121 gctcgggcgt gcgggcaagg agggtgatgc cgaagaggcc ggcgacgagc ccgaggagga
    18181 agagggcgga gtagccgtag gccgcaaggt ccggaaagac ggtcttccag gagtcgatga
    18241 agaacccggc ggcgaggctg atgacgagcg caagcgccgt cgagagggtc agcctccggg
    18301 agaagaaggc cccgagccgg tcctggggta tcaggtcccg catccaggag ttccagccgc
    18361 agtgcgagat tgccgaaaac gatgcgtaga gggcgatcga cgcgatgaga acgaagattc
    18421 cctgctccgg cgagaagaag aacggtatca ggacgatggg cagccagcag aggcgggcgg
    18481 caagcgaggc cgcgacgacc accagcctcc gtttctggac ccggtcgacg acgtagatag
    18541 cgggcatctg caggagttcg gcaagcggcg gtatggcggc aagcagcccg atgacggtgt
    18601 tggatgcccc gagctggagg gcgaaggcta cgaggaagat gccgccggtc agggtcacca
    18661 ttgcctgggt ggcaaggccg tccctgatga cgagtttcag accgcgccgg atctcctcgt
    18721 ccgtcaggct ctctttcgtc tcaaagacca tggtttcccg gatatcctgt tcttgtgctc
    18781 acggggctct tcacctcgaa gggccggccg gcgcagggct tgccgtgccg gaggcacggc
    18841 agccgatcct gcaccgggcg cccttttctg agtccccact atataaaaat aaaattatat
    18901 taatctgttt aaatggatcc ggcggccggc cggggtctta cggcaggtct cccggcatga
    18961 cggcggtcgt ctccgaagaa aacctcctcc tcagctcccc gaagaccacg gcaccctgaa
    19021 gtcgggatag agcgtcaggc cgccatcgac gtagagcgtc tgcccggtga tgtaggccgc
    19081 ttcatcggat gcgaggaacg caaagaccgc ggcgacctcc tccggcagac cggcgcggcc
    19141 taccgggatg tgcttctcca cctccgccct cctggcgggg ttgtccatcc actcccggtt
    19201 catcggggtt gcgatggcgc cgggggcgac cgtgttgacc cggatgccct gcccggcgta
    19261 ctcgagcgca agcgttctcg agagggccgc gagtcccgcc ttgctcgcga cgtagggggc
    19321 gtaatacggt ttcgggatgg tctcgtggac cgatgtggtg ttgagtacga cgcccccgcc
    19381 cccgcggtcg aggaagtgcc gcacggcctc gcgggcgcag aggaacgcac cccggaggtt
    19441 caccgcgagg acccggtcgt aggcgtccat atccgtctcg tgggcggggc tcgcgacctg
    19501 gaccccggcg ttgttgacca ggatgtcgag cctcccccat gcgccgagga cctcgcggaa
    19561 tatccgtttg acgtcctcct cgtgcgcgac gtccccctgg accagcatct ccccgcatcc
    19621 cttcccgcgg atgatgctgc aggtcttcct cgcctcttca agcgttatct ccgcctcggt
    19681 ctcgctcgcg tagtagttga tggcgacgtt tgcgccttcg tccgcgaacc ggatcgccgt
    19741 cgcccggccg atgccggtcg agccgccggt gacgaggacg ttctttcctt caaaccgtct
    19801 cattattcga tcgcccctcg taggtccggg tccggttgcc gtccggcttt tagatgccgc
    19861 aagccgatcc tttgcagccg gatgcctgcg gggggtgccg gacgtcctgc ccgttccggg
    19921 ctccctgtgt tcgaggggat tatgaagttt ttccagggcg ggaagggatc cccggccgga
    19981 tggttgcacc cctccgttgt gcgttctcgc gaaacgactg aaaagtcgta aatagccact
    20041 gtgcgatacc attacaacca gatctcgccg ggcattaccg gaaccggctc cggtgttcgg
    20101 gcagggtcag ggggcaggtg cgcgtcatga cgggagcagg tgacggagag ttgcgatttc
    20161 ttgtcgttga ggacgacggt atcatcgcgt tcgacatcgt gagccgcctc aaggagctcg
    20221 gttgcggaga cgtttcggtg gcggcgacgg gtgcggctgc gattcgccag gcggagcggc
    20281 tgcagccgca cctgatcttt atggacatcg ctctcaaagg cggcatggac ggcatcgaag
    20341 cggcggaggt catccgtgac cgcttcggca tccctatcgt ctacgtcacc gcccactcgg
    20401 acccggccgt caggaaccgg gcaatggcga cgtctcctgc cgggtacatc gtaaaaccgt
    20461 tcaccaccgc ggacctggtc tgcgcgatca ggtctgccct tcggactccg tagcctccgg
    20521 ggaggctacg agcatatcct cttccccaaa ggtgaccgtc accgtcgtgc caccgctcca
    20581 ttcgactgca acgtgcccct tcaggtactc ctcgacgagg agccggacgg tggggaggcc
    20641 gggagcggcg gttgcggtta tgtgcatctc ctcggagatg cccaccccgg tgtcccggac
    20701 ggtgacggtg tacctgcccg cccggctacg gtcgatcgtc acctccatcg tcccggtcat
    20761 cccgtccggg aaggcatgct gaatgacatt gacgacaagt tcggtgagga gaaacccgat
    20821 gaactgtgca agttccaggt gcatctcgat tcccggggcg cgcaccttcc acgcaatctt
    20881 ctccggcggc acgccgtaac tgccgaggag ggtgccggtc aggcgggtca ggtactcctc
    20941 catccggacc cgatcgggat actgcgaccg atagaggtct tcgtgggcga gggcgagcga
    21001 gaggatctgc agctccgtcg cccggatcgc tcttctggcg gcagggtcgg tgaggcaggt
    21061 cgtctgcagg tgcaggatgc ttgagaccac ctggagggta ttgcgcagcc ggtggtggat
    21121 cgcgttgatc agcgcttcct tctcctccag ggctttctcc agatcctgcg ccgcttcgac
    21181 ctcccgggtg acgtcctcga gaatggcggt cacgcccggc gacccatcct cgaagaccac
    21241 cgggacgaac gttgcatgaa actgcaccgt gcgtccgtcg gccttccagg ggatctcgca
    21301 cttgacctcc tgcccccgga gcgcgacccg ggccctctcc ccgatctccg gcggagggag
    21361 gcgatccgga gggaatgccc cgatctgccg gccgacgagt tcggtcctgt cgagacggag
    21421 gagggcctgg aacgcatcat tgatgagcac gacccgcagg ttccggtcga gcaccacgat
    21481 caggtctgag gtcagcccga ggagtgcgga gaccggtacc cggtgcgcga gggtgtagag
    21541 cttcgccgcc gcaaccctgc gcaggtctac ctggcctgca gcatagagca tgctcaggta
    21601 cttcgcggtc acgtttctcc cgatgttgag tctccgggag atctcaagga tgctgaggcc
    21661 caggggctcc tggcgaagga gcatcttgat ctcctcgagt tctgaaatct cgtcaggcat
    21721 gcaggtacct actcctctat accaactcta ctatcataga gattgccagt gacgggcggc
    21781 ggcccgggta agggcgggag ttccgggatt cttgctgttg gacaggcatt ttccggcggt
    21841 ctttcgattc gatcgcttcg caggagtatc ggttcgtaag caatatatgt tacaccaagc
    21901 atacctgcaa ccatatgcat gcacatgagc atgcagatac gcgggcggat ctcacctgaa
    21961 cggatcttcc cggtatcccg gaagtccggc cggggtttgc acccccggct acagccaccg
    22021 acgacagcgt ggcaggccgg atacatcgcc ctgccgggag aggcatcccc ggtatcgctg
    22081 cggtatacga ccgccctgac cgggcgaaaa tccgcccgac acctgtctgc cggcgcaccg
    22141 tttccggaaa gaactggtga agaggcatga tctgtttgaa agacgcgtcg gagccctccg
    22201 gtaccggtac ctttggtgcc ggtggagaat acggggcggg cccggattgg gtgcgcctgc
    22261 cgggacgtgc cgcgggtgga agcgtcaact ccggcatgat ccctgtcggc atctgtgggc
    22321 gcgggctttc aacaatcctt ttcatgactg ctgctatccg ggaggttctc ctgggccaca
    22381 cgaaaggcag ggatgcctga gagggggaaa aacggtgcct gtcccgttga aaccggacgc
    22441 ctcctctatc tgcgagattg caggaggttt ccgggacaga gccggtagag gtggccggca
    22501 gcgggcgtcg ggcggatttt cgcccggtcg aaccgatcgg aggcagatcg ccgggttcct
    22561 ccgaatgtat atggagctcc tcgacagtgc ggcggcgatc gtttccggga ccgtcgcagg
    22621 aggcgcccgg gtttacgtct tcctccagcg actgggaaaa gcagtcgggg tgggccgggt
    22681 ctgcctcgcc ggcgtcgagt acaggccaaa agccggagaa gtccgcttac gcatcctgtg
    22741 tgaatggact gccggcggaa tcgccggcaa agaggaggac ggtcaatccg ttgcgtacca
    22801 gcccctccct cccggatggg cgaagcggct tatggcgggg ttcccggttc atgggcgctt
    22861 tccggcctca cccggttcgg tcctgatggt ccccgtcttc tgcgggtccg aactccgggg
    22921 catcctggag tttcggagtc gcgtggcgcg acgggggtgg cgccgatccg atgtcggcac
    22981 cctgaaggca gtcgccggca ttctcggttc ggtcatggcg ggttccgggg cgccgcatgc
    23041 cgttggggcc tgcagctgcg ccgtcgtcga cgatccgacc gttcccttct gcaggctctc
    23101 gcctgacgga gcgatcgcgt atgccagcga ctcgttctgc gccctcttct accgagcgcg
    23161 cgacgacgtg atcgggatat ccgtttccgc ccttttttca ccggaaatta tgaaaaaact
    23221 gccgaatgtt gccggtttta tggtgacccc cgagttccgg gcccccggag cggacggcgg
    23281 caaacattgc tgccgcagcg tatgccggac gctcctcgac gataggggcg ccgtctgcgg
    23341 gtaccgggtg ttcggttgca agagtaccga attcaagcag caggaggtcc ggagtgtcgg
    23401 gacggacgac cggatgttta ctctcctggc cgaagccgtc tacgatgcgg tcgtcgtcct
    23461 ggacagatcg gccaggatca cctactggaa ccgggccgcc gaggatctct tcgggtacac
    23521 ggcggatgaa gttgtcgggc acgatgcagg gcagctgttc tcgcccgagc acgttcacct
    23581 cctgtacctt gagagtttca agcatgccgt ggcggctgcg gcggagggtg tcgtccgcct
    23641 gcgtttcccc gacgtctcgc tcgtccgaaa agggggtgcc gtgttcccgg cggcgctttc
    23701 gatagccgcg gtgctgacga ggggcgcgtg gttcggcatc ggggtcgtcc gcgacaccac
    23761 cgcagagaag atacgggagc aggcgatcct cacggaagcg gagcggatcg ggacgctgct
    23821 acggatagcg gcacggctga acggcggcgt cccgttcgac gctgcactcg atctgctctg
    23881 cgaggagacg gcccgggcgc tccgggtttc tggcgcggtt atctacctct acgaccttcc
    23941 tggcgacctg ctcgcgccgg cccggtcgtt cggtgtgccc gaggaggtac ggaagtccct
    24001 gccgcccatc ccgcaggcac tctacgagcg agaggtcgaa cgacagggat cggcttttgt
    24061 catcccggac aaaaggccct ggacggacgt ggcgtccgga aaaaaaatgc cggggaaccc
    24121 ccttcgaccg ctcgcctccg cagcaatcct ccacggacgg accctgatcg gggccctgaa
    24181 cgtgatgacg ctcgacaccc cgcgcagcct ctccccggag gagctcacgc tcctgaaagg
    24241 gattgcagac ctggctgcgc ttgcaatcgc cgacatccgc ttttccgcgg agcgggcagg
    24301 gcatgaacac cagttgcagg cgttgctgga tgaaaagacg gccctgttga aggaagttca
    24361 ccatcgggtg aagaacaaca tgcaggtgat tgcaagcctt ctctccctgc agggccgcct
    24421 gacagagaat gccgtggctc gtgcatgcat ccgggagagc gagaaccggg tgaagtctct
    24481 ggcgctcgcc catgagaact tctaccggtc ggagtcgatc gccgccgtcg atgcaggcga
    24541 ctatctgctc cggctcgtaa acgacctgct ccggctctac gccccgccgg tgcatgtgtc
    24601 ggtcgatgtt gagacggaga aggggctcgt cctccccgga gacgtggcga tcccgctcgg
    24661 cctcctcctc aacgagctcg tctccaactc tctcaggcat gggtttgccg gtcggtccga
    24721 aggggcgatc gcgatccgga tcgtccgggc gacgaaccgc cgcctcacca tcgagtaccg
    24781 ggacgacggc gccggcatac ccggtgaatt cgccgcccgg cccccggcga ccctggggct
    24841 gcagctcgtc cggatcctcg cggcacagct cgaaggaacc gtcaccttcc ggaggggcga
    24901 gcccgggacg atcgtggaga tcactatcgg agacggcgcc ggcgagggga ggatgaacca
    24961 tggatagccc gagcgatacg actgaggggc ataccgtcct cctcgcggat cccgatcccc
    25021 tcaccgctgc aacgctctcc ctccggctgc aggagctggg atacgcggtt gtgggtgcgg
    25081 cagcgaagag cgacggcgcc ctggcggcag cagccgcatt ccggccggat gtcgtcatca
    25141 ttagcgccga tctccccggt cccgggagcc cccgggagac ggctctcggg atccgggacg
    25201 ggctcggcat ccccgtcctc gttacggccg cacaccctgt agacacgctc tccccggacg
    25261 gggaggaggg gctctcgccg caggagatcc tcgtcgggct cttcggcccg gacgaccttg
    25321 cctgcgcgat cgaggccgtt atcgcccgcc atcgggcccg gcacccgtgc cgggaatggg
    25381 tcgaggcgtt caggcaggcc gccgagatcg tgccgggttt catcatgctc ctcggcacgg
    25441 atcgccacat caggttcgtc aacctgtccg gagcggccat ggccggacgc ccccgcgacg
    25501 cgcttatcgg actggatgcc gtcgggacgg ccctgccgtt gtccgatcgg gacggcggcg
    25561 accttcggcg gcgtttcgac gaggagcagg agttccacgt tgccgccgac tgcataccta
    25621 tcggcaggtg ccgtttttcc ctccggtgga cctgccagcc ggtgcgggat ggccggggat
    25681 gctgtgccgg ctgggtctgc tccggcaccg gagagggcga cggagcctct ccggatgcct
    25741 ccctgcaggc ggagattctc ctgcggtttg agaagaatat ggagcagctt gcaatcctca
    25801 acgatcggat tcgcaacccg ctccaggtga tcgccgcatt tgccgaattc gcggacgacg
    25861 acattcgcga taagatcctt cttcaggtga aagcgatcaa cgagaccgtc cggcagctgg
    25921 atcaggggtg gcttgaatcg gcaacgatcc gggagtatct ccgcaagcat cacagaatgg
    25981 cgctgtgaat gcgccccgga ggctgcaccg tcgagcgatt cagcgggatc tctcgatcac
    26041 cttccggacg agcgcccgcc ggtccggcct ccccgccgtg agcgcctgga acttctcctc
    26101 gtagatcggc ctcctgaccg agtagaacgt cccgagcgct atcggggcgt cgcggtcgta
    26161 atcccactca cggatcttat ctagagcgct ctcgaacctg cccgtctcga cgtcgccttc
    26221 ctgcatctcg taggtgtgct cgttgtagta gtcggtcagg ttgaagtagg tcgcgcagat
    26281 ctggaggacg tcgacgaacg agaacccccg gtgcagtatc gcctccttga agaggtcttt
    26341 taggtgccgg accttcttcg tgtagccccg ggcgatatgg gtggcgccgg cggcgagcat
    26401 caactcgagg gggttgatgg ggtgctccga gatcccgccg ggcgtggagc ggccgcggaa
    26461 cccaaacggc gacgtggggg tgtactgccc ggtcgtcagc ccgtagaccc ggttgtcgtg
    26521 aacgatgacg gtgatgtcgc tgttgcgttt ggccgcgaat atgaggtggt cgagcccttc
    26581 tgcgtagatg tcgccgtccc cgacatggca gatgatcttg aggtcggggt ttgcaaggcg
    26641 catgccggtg gccgccggta tcgaacgccc gtgaatgccg taaaatccgt tgacgtttaa
    26701 gtagtcgacc agtttcccgt ggcacccgat cccgccgagg aggacgaagt tgtggagcgg
    26761 tatcccctcc tcctccgata attcggcgat cgccgacttc atcatgtgct ctatcgcgaa
    26821 gttgccgcac ccggggcacc aggtgttcgc tgccggactg atgagcccgc cggccggtct
    26881 cccggcagat cgctgcgggc tcatgcgagc acctcccgga ggcgcgcttc cagttcgtcg
    26941 accgcaaacg gccggccgtc gtacttcagg accggccggc cggggtcgaa cccctcctgc
    27001 cggaggaggc gggcaagctg tccggtggcg ttgttctcga cgcagacgac ctttcccgcc
    27061 ccgatcatcg cctccttcca ggcacgggcg gggaacggcg caaagacgag cggctggacg
    27121 acccttgccc cgaaccgttc ggcggcctcg atgcacgccc ccttctccga gccccagcag
    27181 acgaccgttg cgccggcgtt gcgggggccg tagattttca ccgtggggta ggccgcgagt
    27241 tcggccttga ggctctcgcc cttcgcgaac ctcttctcct ggagagccgt gacggccccg
    27301 gcctcttccg tggtgaaccc gacctcgtcg tgggcatagc tgttcgccct gacgacggcg
    27361 ccctctcttc ccgggaacgc gagcggcgag actccatcat cggtccgggc ataccgccgg
    27421 tactccccgg ccccgtccca gagaacgggt tccaggtcgg ggtgcagggc gatcttctcg
    27481 atatcgaacg agtagccgcc ctccgcgagg gtcttgtcgg tcaggatgat cgagggcacc
    27541 tggtacttcc aggagagcat cagggcggtc gacgaccagg tgtaggcctc ctcgatgtct
    27601 cccggggcga cgacgaaccg ggggaactcc ccctggcccg cgttcagaac gaagtggagg
    27661 tcggtctggg cggtgtaggt cggcatcccg gtgctcgggc cggggcgctg ccccatgacg
    27721 atcgtcacgg gtatctccgc cataccggca aacgagaggc cctcggtcat caggcagaac
    27781 ccgccgcccg atgtgccgac cgccgccttc tcgccggcgt aggcaagacc gagcgccatc
    27841 agtatgacgc ttatctcgtt ttccgggtgg aggaccttga tcgagaggtc ttcggcctgc
    27901 ccggcaagga agtgaaggag gtttgacgtc ggtgtcatcg ggtaggcgac gtaggactca
    27961 agcccgccgt ggacgagacc gagcccggcg gcctcgtttc ccgtcatgat cgggagggtc
    28021 ggggcatcga gcggttcgac ggagaaggcg ctctccacgc cgtcgtatcc ccggccggcg
    28081 accctgaggt tcgcctcgag gtgctttgcc gggacgctct cgcggaggac gtctccgagg
    28141 acatcccggc ctatgcccgc cacccgggcg agggcgccga gcatcgcgga gttcccggtg
    28201 atcgggggcg cgtcctcctc cctgacgatc gtatcgatcg ggaggccgta gccctcgcct
    28261 ttcgtcacct gcgaggcgtc gtagatgacg gtcgtgccgc ggtggatcct ctggcggtgg
    28321 ttctcgatgc tgttctggtc gaaggcgagg aggacgtcga ccggagtgcg gtgcgcccct
    28381 atcggccttg ccgaggcacg gatgatggcg aagttgtgcc cgccccggat gagcgagggg
    28441 taatcgaagt acatgtaggt gcggtagccg aggcgggaga agaggcgggc gatggagagg
    28501 ccagcggtat tgatcccctc tcccgccttc ccgccgatca gcacggagta ttcacgcatg
    28561 gttatcaaat gtgcctggcc tgccggcgga tatatacgtt gtgccgccgg aacgcatagc
    28621 cctggaggct ccccgatacg gcctcacggc gacctctcgc gttgccttgc cgggcaattc
    28681 ccggcaaccc tctgccggaa cctcgcggga gccgacagca ggcatccgcc acctcaccgg
    28741 acgaccggcg acgccgtctc gtcgaagggg ttcgagcgca tcgcaagcga gaagaggtaa
    28801 gggtagttct tctggaggta ctccatgtag gcgagccact cccgggcgag gagacggtat
    28861 acccgctcga tatccacggt gaggtggcgg acgtcgcttg ccggaagccc ggcgaagtca
    28921 tcccggcgtt cgagttcttc ggtcaggtgg aagaccgccc tgaggagatc ggtgaaggac
    28981 tcgtgctcca ggagagcagg gttctcgagc agccggagga agaagtcccg ccgcccttga
    29041 aggtagcggc ggaggtcctg caggtccgcg gcatcgacgg cgatccgggc gtggtggtgc
    29101 tggaggcggt cgcacacccc ggcaaacgtc tccggcgtcc aggcctccgt cacgatgagc
    29161 ccttccctga gttcgggcag ttcggagtcg cgatccgata tgaaggcgag gagccaggtc
    29221 ccggcctcgg agaagaaggt cccgatgacc atgttcagtt tctcgagcct ctcgcgcttt
    29281 gcccggatgc tcaggagctg gttcaagata agggtgacga ggaggacgtt gatcgggagg
    29341 aacccgaggg cgttgaatat gtaggtgtag gtgttctccg gatcgccgag caggaggtac
    29401 tttaccgtgt agatggccgc cgacgaggcg accagaacga taccgaggcg tacttcccat
    29461 gtccggaggg tgagcggcat gcgcagggga atgtacgcac ggcggggcga ataaaggttg
    29521 ggccgcgggg gcccttcaga cccggaggcg cgacggcgcc ggcaccttga ggggcacggg
    29581 aaccttctct tcccgcgcga tctccgccct gacccaggcg gcaaggtcga ggaggcccgc
    29641 ctcgagggag acctccggct cgtagccgag gacctcgcgg gcccggctga tgtctgccgt
    29701 gcagtggcgg atatccccca cgcgccccgt ccccgtgatg tcgggagaga ggtcctgccg
    29761 cccggttatc gccataagcc ggctcgcgac ggtccaaaac gtgtgaggtc ggccgctcgc
    29821 gatgttcaac gtctcccccg ctgcacgggg ggactcgagg gccaggcgaa aggcgcgcgc
    29881 cgcgtcgtag acgctcacga agtcgcgctg ctggtagccg tcctcaaaga ggcgcacggg
    29941 aaggccctgg agcaggcgga acgcgtactc ggtcagcatg ccggagtagg ggttggcata
    30001 cccctggtgc gggccgtaga ccggaaagag ccggaggatg gtcgtcggta tgccgtaggt
    30061 ctcgccgatc atccggcaca tctcttcctg gtcgtacttg gagacggcgt agaccgaaga
    30121 cggcgacgcc tccttcgtct ccggcgtggg aagagggtag atcacctcgc cgtccgggct
    30181 ccggggctcc cagtccccct ttgccgccca cccgggaaaa cgccggacct tcgggtagac
    30241 agtgccgttg tacgagcagt agagcccttc cccgtagatg gcgctgcttg aggcgacgac
    30301 gagctgcctg atggggtggt ccagcaaagc ctcgaggagg acggcggtcc cggatgtgtt
    30361 gacgctcagg tacttctcga tccggtacat gctcgaccgc tcccccacga ccgcggcaag
    30421 gtggatgacg gtgtcgatcc cgtcgagagc ctcccgcagc cggtgaggat cccgtatgtc
    30481 gccgacgacg acctcggccc gccggtcaag gtgcccgggc tcccgcctat ccgggccgtg
    30541 catccgcggc gtcaggttat cgaggatgcg gatatggtag ccgttctgaa gcagttccgt
    30601 tgcgatgtgc gatccgatga accctgcccc cccggtgacg aggagggtcg gactggtatc
    30661 cgtatgcatg gtgtatcagg gcacacctga tgcgctcttt gcattaaata ttatcgcgaa
    30721 tgcgaaatat taatgaatcc cgtatattcc agctatcgag gtcgttctcc accttctccg
    30781 atccggtcta cccggccggg ccggggaagg gcggcgccca tcccgcctcc ggttcacgag
    30841 tcaaggatcg cctcggccgc atcactgtag gcccgcatga cgtagggtat gccggagatc
    30901 gcctccgcct cctccgtcag ggccatcagg tcgctccggg cgaccgtccc gaggctgaag
    30961 ttccgggttc cggccatgat ctgctggagc cccgtccgaa acttctgggt ataggtgtag
    31021 atcccgaccg cgccgagcgg gatctcgccg atccggtcgg ggaacctctc ccggagctcc
    31081 tcgtagcaga cgaagatctc ctctttcgtg cggccgtact tcgagacggt ctttgggagt
    31141 tcgcccgcct cgatccacgt tgctatgttc ttcccgacca tgccgggtat catgagggcg
    31201 cggcccatgc agacggcccg gacgtagggg ctccccatgg caagcgcctt gaagacgttc
    31261 gcttcgtcgg caaacccgcc ggcgatggcg atatccggga cccggatgcc ccgctcccgg
    31321 agccgttcgc agaaccggca ggcgagggac tcgatgtaga aggtcgggat gccccactcg
    31381 ttcatcatcg gccaggggct catccccgtg ccgccgggcg cgccgtcgat cgtgaggagg
    31441 tcgatctctg cctctgcgcc gtagcggatc gccatcgcga gttcgacggc cgagtaggcg
    31501 ccggtcttga gggtgacccg cttgaaaccg atatcgcgga gccggtcgac ctcgtcgagg
    31561 aacccctcgc gggtgacgaa acccacgcgg gagtgccgct cgaactcccg tagagcgccg
    31621 gcctcgaagg ccttctgcac ctcgtggagc ccggggtcgg ggaggacgat gtagccccgg
    31681 tccttgagcc ggttcgcctg gtcgatatcg ccgaccctga tctcgccgcc gatgcacttt
    31741 gcgccctgtc cccacttcaa ctcgatcgtc tccaggttgt gctttgcgga gacgtactcg
    31801 gcggttccaa gtcgcgtatc ctcgacgttc aactgcacga gaagttcgcc gtaccggtcg
    31861 tgaaaccgcc ggtagatctc gatccggcgg tccatctcgg gagacctcgc gaccctgccg
    31921 gcgtggtcga gcaccagccc ggggtcgacg ccgcagacgt tctccccgca gacgagcgtg
    31981 atgcccgata tggcagcccc gatggcgaac tgctcccagt tcgcgcgggc gctctcggtc
    32041 gacccgagcg ccccggtgaa gatcgggacc cgcatcggga ctttcgcgct ccacccgtac
    32101 tcggtccggg tatccacggc ggggaatacg gcggcgtcgg ggccggcctc gataccttcc
    32161 ggcagccctt ttgcgccgac cgcgtagccc tggatgttga ggtgcgagta gtcgaccggg
    32221 tagttcttat cggcgccggc cgtgatctcg ccgaacgggc ccgggtagag ggcttctctg
    32281 ccgcggaagg aggaaagcca gatctcgcag gcccctttgc agccgtcgat gcaccgggta
    32341 cagatgcctg atgcgggcac gacgtcacgg gaacggttgc tggttcccgt cgcttcgttc
    32401 gcgttcggtc ttcgcaggtt catcgttgca ccagttgacg gatagaaatt tccttccacc
    32461 atatataaat attcctatcg gccggggccc gcaccggtat ttataccgtc gtatcgccgg
    32521 ggtgccggga gcatgacgaa agggtaaaat atgttggtgt acatgattgg cttatgaact
    32581 tcaggttgct acctgctctg atagtgctcc tgctcgcgtt ctcactcggt gccgcctgca
    32641 cgggctccgg cggtgaagcc gggccgtcca cgccgggaac cgggaccccg gcggccacgg
    32701 caactcccac accgacgacg gaggtctcgc ttgtgcccgg cccgacgcag acgataccgc
    32761 cgggcaaaga ggttacgttc cagatcacgc ccggatatcc ctccccgttc acgcatgacc
    32821 tcaccatcac tttcaacggc ggcaaaggtc aggactacgt gaacggcatc gcgctccgcg
    32881 tgaccaagtc gaacggcgag gtcgtcgaga agacgctgca gccgcgcatc ggcgacgcgg
    32941 tcaccatcaa tgaggcagaa ggggataacc gggtcgagat aaccgtctcc ctcgtcacgg
    33001 gaggatccta caaagttacc gacgaggtcg tgaaagtccc ctgaatcccc cgacctctat
    33061 tttctcgcga ttatagcctc aatcccgggc agccgactct ttctccacgc tccaccgtac
    33121 gaggagaccg tcgtcgagcc gctcgatctc gatgatgcgc agccgcgtaa aatccacctc
    33181 cctggtaaaa ccgtcgccgt cggccggcgt gggagcatcc tttccgccga tgacgatgtt
    33241 gccgacgaac gtctggagtt cgtcgacgag cccctgcgca aagagcgccc agatcagggt
    33301 cccgcccccc tcgaccatca gccgcctgac cccctgtcgg tagagttccg cgagcagggc
    33361 cgcgaggtcg accgtctcat cgccggcgat gacgacgacg gcgtgtttgc cgagggctgc
    33421 caccctctcc atgtccgccc tgcgcgagac ggcgacgatc cgcttcccgg ctcccttgtg
    33481 gaggatatcc gcatccggag gagtccgccc ggcgctatcc acgacgacgc ggatggggtg
    33541 ctcgtccctc ccggccgccc gccgcccggc ccggaattcc ggcgatttga cggtgagcga
    33601 ggggttatcg gcgagcacgg tgccgatgcc gaccataacg gcatcgctct ccgccttgat
    33661 ccggtcgacc cgtgcgaagt ccgtacttcc cgatatcctc acctgccgtc gctctcgtgt
    33721 cgatatcttc ccgtcggcac tcatggcgac gttgacgaag acaaacggcc gcatattgta
    33781 gaactatgca acgatccaca tatatgctct ttcctgctgg gtactgcgcg gcgcctctcc
    33841 tcctccctga accgggaaac gttcgacata cggctccccg ggaggcatgc gcatacattt
    33901 cgttccggga aagattattt aaccctttac agaatacttc gaacagcata tatacccttc
    33961 ccggagaatt ctctgtatcc ctcatgaccg taaaccggcg ttttccgggc cgtctccggg
    34021 aagatctctt cacagggacg gatcttgtca agatgatcgt gatactgtcg ttctcggcag
    34081 tctcattcat gctgacggtg atcgcaccgg attacggcat atatcccgca aattccctga
    34141 tatactgcat cccgatcctc ctcgccgctc tctggtatcc agggcaaggg ttcagggtga
    34201 ccgcgctcct ggtcgccggg ttcgccctca tccgcatata cttcttaacc ctcggcttcg
    34261 ccatagaccc ggtgatgacg gggctgaaca tgctcttctt cttctgggtc tttggggcga
    34321 cggttctctt ctcccaggac ccttacctga ccatctcccg gtgcaggcag gtcgtcgaga
    34381 atacgcgcga tgcgaggctt ctctgcgacc ccgagaccct ccgggtcctc tgtgtcagca
    34441 ggcggtgtgc cgatatactc gggtacgcgc cccccgaact catcgggatc ccggtggaga
    34501 agttctggcc ggacgagagc gagagggccc ggttcatcgc ggagatgaag cgggagggct
    34561 acatcggcaa catggaggcg acgtttcgca ccaggaacgg cgacgcccgt gcagttctca
    34621 tatcctgccg gtcgctggcc gacgagaacc tcgttttatg cacggtcgtg gatatcggcg
    34681 gacacgccgc ccttgtccag gaaaacgggc ggttgatgga actcattcac cagtctaacg
    34741 acatattttt catgcaggat gttgcgggcc gcatcctcca cttctcctgg ctgcgcgccc
    34801 ccgagtacgg gatctcccct gaaggtctca tcgggcaggg cgtggatgct ctcctctccg
    34861 ctgacctcgc cagggagcac gcggcacggc tccaggatgt ggtcgagggc aaaaagagca
    34921 cgtgttatga tatcagcctg gaactggcgg gaacccgcca cgtcttctcc atcacgatgg
    34981 ccccctatta cggggtggac ggcaccctca tcggtgtcgc cggatcggcg cgcgacatca
    35041 ccgaggtgtg gcggcagagg cttgcctgca ggcagatggc cctggagata gaccagtgga
    35101 aagggctggt tgccaccctc tcccacgagc tgcgcacacc gttgcagccg cttatcgggc
    35161 acctccagat gatcgtcgag gatcccgggt attacggcct ttccgaagag gcaaaagggc
    35221 cccttgcgac ctgccttgcg tgtgcccggc aggaacaggc ggcggtggag aagatggtcg
    35281 gattcggctt gcttgcagcg gattccgtcg aactcgtcat tcagagcgta cctctccggt
    35341 cgctcgtcga ttccatcgtc tcctccggcg gttacgaccg ggaggcggag ttttgcaacg
    35401 atataccgga gaccgcctct ctccggggag atcgcgatcg gctttatgag gtgatagaga
    35461 ccctggtatc gaatgcagtt aagtataatg aaccgccaaa gaaagtatgg gccagctaca
    35521 cagaatcgaa tagaaaccat tatattacgg tatgtgataa tggcatcggt atctccgcgg
    35581 atgccgtcga atcgatcttc gggccgttct ctaccggggg caccgagcaa ccgaaccggg
    35641 aaaggaggca aacggatcct ggcctctcga tcgcaaataa gtatgttcga ctgcacggag
    35701 gggatataac ggtgagaagc gtggtgggcg aggggagcac cttcaccatc aggataccaa
    35761 aggaggtgta aaagagtggg agataagatc atggtcgtcg atgacgacct gccaacactg
    35821 gaagtaatgg agctgctgct caagaagatc gaccgtgagc cgctcctggt tcacaacggt
    35881 tgggacgcct tgaggatgct caagaaggag aaacccgcgc tcatcatact cgacgtgatg
    35941 atgtcgccca tcgatgggtg gcagttcctg gaggagctga agcgcaacga ggagttcaag
    36001 gcgatccccg tcctgctctt caccgcaaaa cacgtctggc ccgaggagta ctcccgatat
    36061 gcgggtgaca tcgtcggggt tcttgagaaa cccatatcgc tcgccgagtt aaagacggct
    36121 cttgagaggg cgcttcccgg gagcgccgct tctcacagag ataatgatac tcacgcacga
    36181 acacaggttc gaagcgggta acttttttcc cgaaaacccc ctgagaagat cttccgtccg
    36241 gcgataccgg tctgcttgca ccctccaccg aaaggcattt cacttgcccg gggtgtaata
    36301 cgggcaggaa ccccgatgat cgttacggat ctcccctgct gcccctctct caaagagtac
    36361 cgccagatcg tgggagcaga acagttctcg gccattgaag agcttgccgg ccgcctttcg
    36421 ggcatacgga tgcaggagat caactcgacc cgccagggcg gcggcgtcgc cgagattctg
    36481 gcttcctacg tccccttcct gaacgcgctc ggcctcgaga cgacctggag cgtcatggag
    36541 gcaaaacccg cgttcttcga tgtcacgaag accctccaca acttcctcca gggccgggac
    36601 gggttctcgc catcgatgat cgagacctac tgggaggccc agcggcagaa cgaaggcctg
    36661 atcgaggatg acgccgacgt cgtcaccatc cacgaccccc aaccgctcgg cctcatcgag
    36721 tttctgaccg ggagggagcg tgagcaaaag aggctgctct ggcggtgcca cgtccagctc
    36781 gagaccgtcc ctaccgggac cgcaggcagt atcggcaaca tcctgcgccg gctggtcgag
    36841 aagtatcacg caagcatatt ctccagtttt cagtaccttc ccctctggcg cgtgccgggc
    36901 ttcatcatac cgccgttcat cgaccctctc tccgagaaga accgcgacct tcccacggcc
    36961 gaggtcgatg ccgtcctcgc aaagtacggg atcgacgccg aaaagccgat cgtcacccag
    37021 gtctcccgct acgacgtctt caaggacccg gtcggcgtca tcgaggcgtt ccggaaggtc
    37081 cgccggaaga cgccctgcca gctcgtcctc gtcggcgggc gggcgtgcga cgacccggag
    37141 tgtttcctcg tcctgcgcga ggtccgcgag acggccgagg acgaccccga tatccacgta
    37201 ctcgacctcc cgccggacag ccaccgggag atcaacgccc tccagagggc gtcggacgtg
    37261 atcgtccaga agtccctcaa agaggggttc gggctgaccg tcaccgaagg gctctggaag
    37321 gggaaacctg tcgtcgccgg caacgtcggc ggcataccgc tccaggtccg cgacggctgg
    37381 aacggatacc tggtctcgac ggtccaggag accgccgacc gggtactcta cctcctccgg
    37441 cacccggatg cggccggaga gatggggata cggggccggg agttcgtgcg ggagtactac
    37501 ctccttcccc ggggcgtgca ggaccacctc gcggtcgtcg accaggtggt gaacggcaag
    37561 atcaccgccc gcgagagcgt catctgctac cacccccagg tggtgacggg caggatggcg
    37621 gcatgtgcgc cccgggattg acggggcgct ccctgccctg ttatatacta tggcgtgctg
    37681 agggtgggcc cggtgatagg agatgaagat agcgattgcc agcgcggggg cgggggggct
    37741 tgacgatacg gtgtcgccgg tcttttgggc gtcatcctcg ttcaccctga tcgatgcgga
    37801 gaaccaggag atcctggccg tggagatcgt cgtggctccg gagggctgtg cacccggggc
    37861 ggccgctggc cggttcgcgc gcgaccttgc cggccggggc gtggatgccg tcattgcagg
    37921 ggagtttttg ccggaagaga ccggcgtgtt ctcgtcggcc ggcatcggca tgtatcgcct
    37981 tcaggatatg ccgatccggg atgccgcaaa gcaggttttg aaccttgccg ctgataccct
    38041 gcgatctccc gggcgcgggg ccgtgccgac ggactacgtg acccggacct acggcggagg
    38101 ggtcggcctt ggcagccggc ggcggacggg cggggggccg cggtagcccc ggtcacccga
    38161 tgggtatctc tcttccgccg ggtccggatc cgccctccac cttcttcagg ctcacctcaa
    38221 ggacgccgtt cctgtacccc gtcgcggcgc cttcggcggt cacgctcgcg ggcaggcgta
    38281 tcaaccggct cagggtgccg tctcccgccc cctctccggc ggggccggcg cgccttgccg
    38341 ttatccgcag ggtctgcggg ttggggagcc ggatggcgat cgtctcctca tcggcgccgg
    38401 gaagttccgc gatcacgatt acccggtcgc cgcggtcgag tatgtcgacc cggaagcctg
    38461 cggagaggat ggagtagtcg cgggcggtcg cgggcagact gtcggtctcc cgccgcccgg
    38521 tgccggatgg ctcgtcttcg cttcctgtca tggtccctct ctctcgatcg gatggccggc
    38581 cggccacccg gcggtacctc gtctgccggc acacttaacc ctttgtaccg ggggcggcct
    38641 gcctaaccct ggcgggatat gcctcgcgaa tccaggaggc ccggccgcca acagactctt
    38701 ctgtcacgtt ttttcatcgg ccgttaggtt tttgtggtgt gaccggtatg gtggggaacc
    38761 cccccggtct ttcggcgtgc agggtgatcg gaatggcctg gccctcttca ccgctgcacc
    38821 gccggagtgt ccgtactggt gggatcggcg gggttctgga ggagagagaa tggctcagtt
    38881 taaaccgttc gatatggagg gcatgcctct tgaggagcag cccatgacct ggaaggatat
    38941 gtctacggcc ccgtatgaca agaaggcggt cgacgcctac acccggaccc gtgtcatcct
    39001 catgaacggg atcgagaaca acgccgtcct gatgtcgcac gccatcgcgc ggatgcaccc
    39061 cgaacccgag gtgaagaagg cgatggccat gatacggcgg atcgactccc agcaccaggt
    39121 ggcgatcaac tggctcaacc cggcggacca gtcggtcatc gagaccacgc tcggctacga
    39181 gcaggtggcg gtcgatctta cggcgaacct ggcgaagaac gagccggatc cctacgtgaa
    39241 gcagacgctg gactttgcgc tgctcgagga cttcgaccac ctctaccgct acagttgcct
    39301 ctacgactac atcgagggcg gcgacgccga ggcgatcgtc cagggcaaga ccgatgtgaa
    39361 accgggccgg ccgacgagca tcgaacaccg ccacccagac gactcgatgc gcaagcacta
    39421 cgataaggat acggccgaca tcaagacgaa gatgaactac gtcacgatcg tcgccgggga
    39481 gcagcagacg atgctctact atcgggcgca cggatccatg taccccgacg atatcgcgcg
    39541 gcgactgtat gccgagatcg cagagatcga ggagcagcac gtctcccagt acgagctcct
    39601 cggcgatccg cgggagacga tgcttgagaa gctggcgctg gtccagctca acgaggctta
    39661 cctctattac tcctacgcgc agcacgagag cgacccgagg atcaagggga tctgggagac
    39721 gcatatgaag atggagatcg cgcacttcaa cgagtgcgcg cgactcatca ggaagttcga
    39781 ggggcgcgac atccatgaca tcctgaaggc cgacgtcgtc gaaccgttga tcgttttcga
    39841 gtcgaacaag gattacgtcg accgggttct cgatgcgcag ctcgatctta tgccgaacaa
    39901 cagggagtac gtgcggctcc gcgaccttcc cgacgactgg gcaagcttcg gctaccaggc
    39961 gaaggtgaac gcgaagggcg ccccgagcga ggagatcgtc tcgaaagccg gccgggagct
    40021 tgcacagcgc gatcaggcgg agaagatcag gaaggtcaag caggagatgg cacgggagat
    40081 ggagaagggc atggcggcag tgccggcccg gtgaacactt ttttgtgtgg cggccggggc
    40141 agggatgtgc gtggacacgt gctgcgccct gacccggatg gtgttctgca accggggcgt
    40201 gttctatcgg gtcctcacgg cccgccccgg cggtggcaac ggggctcctc acggcctgat
    40261 aatcgtcatg gcgctacttg ctccgatcga tatatttata atattacgcg catatgagta
    40321 atatgtcaca acagaggtga agaaaagatg ccaggatttg atggtaccgg gccccgggga
    40381 atggggccga tgaccgggcg gcgcatgggc cggtgtgccc tgccgccaca gacagaaaac
    40441 agccctgccg gggaagcggg cgaaggtgcc ccggagactc cggttcagca gccgcaggtc
    40501 tacggcctcg ggcggggcgg cgtcccgtgg ggatgcggca gaggcagagg attcggcggc
    40561 ggcggacgca ggcgctgccg gtggtaaacg cctgatgcgg gccgggttat catccggcct
    40621 gcaccggcaa gagcgagcat ggccggcagt gattgacccg ggcgcaagac ctgtatggat
    40681 cggagatgtg gcggaccccg ccggcacgag gcgggcaaac ggcggaagta ttctgcctga
    40741 atgttaacga atacgggcga attcattccc ggatcggatc ttaccggcgg atctcttgtt
    40801 tccggagttc ggcagcgcgt tttgcgcttc gcgggcaaaa gcaagcgttt tttccgggtt
    40861 gcggaggccg tctttcttga tgccggtatg cacgtcgacg ccgtaggggc ggaccttccg
    40921 tactgcctcg cggacgttct ccggcgtgag ccctccggcg agtataacgg ggattgtcga
    40981 gtttgccacg atcttcgcac tgatgttcca gtcgtgcggg atacccgtcc ccccgatccg
    41041 ctcttctgtc ctcgagtcga ggatgagggc gtcggcatag ggctcgtaga gttctgccgt
    41101 cgctatggcc gattcatccg tcacatgaac ggcctttacg atcttcacgt acgggagggc
    41161 accgcggatg gcggagagtt cggacggttc gacggtattc tggacctgca gcgtcgtgca
    41221 gtgcgaatct ctcgcgagcc tgacgaggtc ggtcgtctcc tgcaggtggg tgaccgcgac
    41281 cggttcgatg aacggcggga gatgccggat catatccgca gcctctgccg gcgtcacgac
    41341 atcggcgctc cggtaggtca cgcccatgat gaaccccacg gcgtcgcaac cggcatcgac
    41401 cgcacactgc atatccgtaa ggctcgccgt tccgcagaat ttgatgcgca tcagatcctc
    41461 tctgtcgtcc gggaagatag ggtatgcgga ccttcaaagg ttgccggccc tgcacgagcg
    41521 gtgagatgag gggctgctcc caccggggga ggtcccggca gagccggctc tgctgctccg
    41581 gttctcgatc gccggcccgt tacgaccgtg ccgggacgag atccgcgatg acgatggtcg
    41641 gctcgtgttg cgtgagcccg gtgatcttcc tccgaacttt tctcagtcgg tagcgatcat
    41701 cggcacctcc cccgcgaggg agtgtttttc ggggtggggc ggcagcgctg cttgctgccg
    41761 ggggatcgtt cggcggtacc ctcacggatg gccggttgcc gataggtctt tcttcaggcc
    41821 gagaaccttc cgaagttccc ggcgcgagct ctcaccgtca agaaccgcgg cgatctgccg
    41881 ccatctgata cggcaaatgc cccgcccatc tcaagtcttc aggttgccca aatagcgata
    41941 gccgagggca tcgcggaagt gggcgacgac ccgctcctgc gttcggtgct cgcgttcgcc
    42001 gacggagatc atggggtatc gcctccgacg atctcccagc ggccaccctt tgccgggccg
    42061 atgcgttgca cttttccaga ctttttgagt tttgcgagtt gcatctctat ggcccgggtg
    42121 ctcctgccca gtgcttcggc catctcgggt atggttatat ccggctgctg tcggatcatc
    42181 tccaggatta accccgaagt tttccccgaa gttttccccg aagttttccc cgaagttttc
    42241 cccgaaggtt cttcccta
//
LOCUS       DAOSEE010000003        39431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_553_length_39431_cov_252.178738, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000003 DAOSEE010000000
VERSION     DAOSEE010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 39431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39431
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_553_length_39431_cov_252.178738"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="PLI76_00535"
     CDS             complement(<1..782)
                     /locus_tag="PLI76_00535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOI60545.1"
                     /translation="MYRPKILFLTSSLSSFVEGDLRMLQENYPIREVIAGGGPAGSLV
                     QRLSLGLAIFFGVLRTDITFSWFAHNHSYLAVMASRLLGKKSVVVIGGYEVANEPEIG
                     YGAILDPELAKRVHYIIEHADCILAVSEFSKGEILAVAQPRRIEMIYNGIDISVFSPE
                     GEKGDVVLTVCFVSTENIRVKGLDTFIDAARQLPEVRFVLIGQALDGALEILKRDAPA
                     NVEFIGAVGRSELPDWYRRAKVYCQLSHRESFGVALAEAMSC"
     gene            complement(794..1873)
                     /gene="wecB"
                     /locus_tag="PLI76_00540"
     CDS             complement(794..1873)
                     /gene="wecB"
                     /locus_tag="PLI76_00540"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOI60546.1"
                     /translation="MKIVSIVGARPQFIKCAPVSRELRKEHEEILVHTGQHYDHGMSA
                     VFFEELAIPEPDYNLGIGSGTHGRQTGAMLGAIEDVLEKEEPEIVLVYGDTNSTLAGA
                     LAAAKMHVPVAHVEAGLRSFDRRMPEEVNRVLTDHASDILFCPTATAVRNLAAEGVTE
                     GVFLVGDVMVDAMNHNRAIAEERSRILEDAGVRPGEYLVVTVHRPSNTDSRENMAAII
                     GALGEAGVPVVFPVHPRTRKFLLGYDLLAAMPENIRITEPLGYLDMLRLMAHAKKILT
                     DSGGVQKEAYMLGVPCITLRENTEWVETVEAGWNVLVGAEREGIVDVIRQFSPGFLQK
                     RIFGEGDASLRIREILTGYQDARAG"
     gene            complement(1876..3312)
                     /locus_tag="PLI76_00545"
     CDS             complement(1876..3312)
                     /locus_tag="PLI76_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOI60547.1"
                     /translation="MSNTLQSIVDARGPIRKIGVVGMGYVGIPAAVLFADAPEFESVL
                     GFQRASASSGYKIEMLNRGESPLKGEEPGLEELLGKVVNAGKFRCTSDFSEVRGCDAV
                     TLAIQTPFLDPKDLIPDFSALNEGLRAVGRHLSEGTLVVLESTVTPGTTAGMAREILE
                     SESGLVAGEDFCLAHAPERVMVGRLLRNIREHDRIVGGIDDVSTARAVELYRPVLTTG
                     EIIPMTATAAEVTKTAENAFRDLQIAAANQLALHCEAMGVNVYDVRAGIDSLKGEGIT
                     RAILWPGAGVGGHCLTKDSWHLERGAQVLGRGDLWYPHGAESIFGVARKINEFMPRHM
                     VHLTIEGLRRAGKSTEGTTIALLGWAFIQNSDDTRNTPAEPYLAAMEEAGAAVRVHDP
                     FVNGYPGLEVSHDLAGTLAGADVVVVFTAHHHYTALDPARVKELSGREHPVIVDGRNV
                     VDPDAFIRAGFVYKGIGRGDKNSHPVRR"
     gene            complement(3321..4271)
                     /locus_tag="PLI76_00550"
     CDS             complement(3321..4271)
                     /locus_tag="PLI76_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOI60548.1"
                     /translation="MSIRGNTVGGDNLDAGVIGVGMMGRNHARVYSELKAVDSLYLYD
                     LNEKATRDLAGAFEAAASPSLEDLLRSVDVVSICVPTPYHFGVAETVIGAGVPFLVEK
                     PICATAEETRRLIAKIPDGFAAGVGHIERFNPIVPEIKKIVRDPLYIEMKRHNPASSR
                     VSGSSVVEDLMIHDVDIIRNVLLPGGAYRLAGSGNQDVCSALFSFGGTPVYLSASRKS
                     SKKIRMIYIEEEEFTVEGDFMAQEIYIHRKPGQYSVENERYVQENIIEKVLVNKQEPL
                     KLELSTFLDCVARNREFPVSPAQALLNMEICEDVARCFTA"
     gene            4472..5917
                     /locus_tag="PLI76_00555"
     CDS             4472..5917
                     /locus_tag="PLI76_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HOI60549.1"
                     /translation="MRVDPVQRQSLISFGSTIGLTAIGFLSTMFFTHTVGLSILGAYF
                     LFVAYFSVFNLIGDGGFGGAAVKRISEGEDQNAYFTAFTLLRVALTVVSVGFLLIAQP
                     YLVKLTSAGVFPWLILALVVSTFTNIASNSVYGRGMVGVNQIGSLIDTLARILVQVAA
                     VVLGYGIAGLAGGFVAGILASGLFNLRFLDLSPAPFRRSHLKSLFAFSVWTFLSSSGY
                     LVFSYADTIMIGHFMTDADVGVYRVALQLTSIATFVTVALHTTLYPKVSYWGKQGDLS
                     SVERALARAFTYSLLLAVPVVAGGWLLGERLLYFFYTASATAGAGALAILLLVQVAHV
                     FMFLQTMCLNALDRPRDSFRVTAIAVAANVGLNLLLIPAYGIVGASAATLVTMILNAV
                     LAHRALSRSIRIRIEPRAVGHIVLAALVMAAVVVVCTFIIPLSNVFVTLGAVGLGGLV
                     YILALLRLDEGIHDELKDLAGQLGIPWPGVL"
     gene            6120..6557
                     /locus_tag="PLI76_00560"
     CDS             6120..6557
                     /locus_tag="PLI76_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOI60550.1"
                     /translation="MKKEIRELEHEEFPLAEKVWTHYRDQKADPRRERIFGVFVDGVL
                     AATARCTRHPGGLEMDCVFTLDEYRGGGYAREAVEMLLDECGSETIYIHSTLPLVGFY
                     GKLGFVPIPEASLPASIKERFVFCFGEMAGCNVAPMMRNPTSG"
     assembly_gap    6615..6714
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6715..7642
                     /locus_tag="PLI76_00565"
     CDS             <6715..7642
                     /locus_tag="PLI76_00565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOI60551.1"
                     /translation="RSPAYRAAAGRGNVSAAGPRESSVPLPVRTTANLMCDEARQAGS
                     FGMASIDYVVLTWNSAATLELTLSSIRNYGRPNRIIVVDKHSTDRTTAIAGEYDCDII
                     SSGACLGAARRLGAREARTDLVAFVDSDVELLPEWEEVLRASADGRYPDAGVIGALYN
                     DGYLRPITDPVSFLGGNGAFGCSVTRRRCVLACTALDGLSSGEDALYAEFLAEKGLRW
                     YILPIYVNHHRELGNVPEALRWRWLGAGLRRRRGFRASVCKSILGGAVTGLRVNGLDV
                     SYRENLTLRLNYFVGYMLPGKYFEVERKERRP"
     gene            complement(7653..8105)
                     /locus_tag="PLI76_00570"
     CDS             complement(7653..8105)
                     /locus_tag="PLI76_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5814 domain-containing protein"
                     /protein_id="HOI60552.1"
                     /translation="MIADKARFRAARKLERAAGFRLPDHVFSGAFLESLGKAINFEHL
                     DRRMQEQLLAFFRDFMDCKCKNAPFCGCPERKFTLAIIEFREMGLDHRQIGAHLLDEY
                     GIDLYPADILSFLEDSVHMLEAIRDVAELQGREKLAENAIEHIKKIEH"
     gene            complement(8102..8728)
                     /locus_tag="PLI76_00575"
     CDS             complement(8102..8728)
                     /locus_tag="PLI76_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2150 family protein"
                     /protein_id="HOI60553.1"
                     /translation="MAKKAKAKQAEPMKLFYIFYNQERWDNWIKTLEGADFEPAEGED
                     VSEGEQMLYSFSEDITLSVLKIIRLYQNDRFTKEEAIAKLDDVELIVMTGLPEGELED
                     IIGSLQLSLLVLFTACRKYIEGEFDKDIKALVKKGRGIDEENFEEALEVAANIGAAVV
                     DGATCCAKYIKDDAENPGLFDEWLIEIETMSNAMKSLAKFDEEPGEAS"
     gene            8843..9619
                     /gene="larE"
                     /locus_tag="PLI76_00580"
     CDS             8843..9619
                     /gene="larE"
                     /locus_tag="PLI76_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HOI60554.1"
                     /translation="MTHDADLALLLKSCEPIAIALSGGTDSSVLLAYARRHGIRAIAI
                     SVDTGLTPPGELEAARVLSERLGISHIVIPLDALDIPAVRENRPDRCYACKRAMMEAV
                     VREAERRGCRAVVDGTHADDRSAARPGMRALAELGIRSPFAECDIGKKDIEALAADLG
                     VPVRPPSACLATRIPAGDAVTRECLALVAAAEALIAKEIPGTIRVRCIGEGARASIEA
                     DPAYHDRLEELLDAVRNLGFEDAVIASGGYREGGADAWKR"
     gene            9607..10776
                     /gene="thiI"
                     /locus_tag="PLI76_00585"
     CDS             9607..10776
                     /gene="thiI"
                     /locus_tag="PLI76_00585"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HOI60555.1"
                     /translation="MEAVMIRYGEIFLKSETVKRRFISIMTENIRLALEAKGLSHRIE
                     TPRGRILIFGDEPRRIAAAVAGTFGVVSASVCTMTSSDIDTIAAVAVEHAGRHLRPGM
                     SFAVRARRSGVEGFNSQELAAAVGSAILDRVPEATVDLSTPDYEVFVEAREFGGLVYD
                     ERLKGPGGLPYGTQGEVMALLSEGIDSPVASWLMMRRGCRVVHVHMHGGRFGGADGEE
                     TVLRHHARLSAWVPGHPLDLLVVDMEPFFEVIIALKEPRYRCILCKRFMLRVAGILAE
                     ERGAYALVNGDNLGQVASQTLANMAVIAPAASVTVLRPLIGFDKEEIVERARAIGTFE
                     ARAGSTGCTVAPRYPSTAAPAATIVDIEEKIGAKDLAARAAGTVRVYRAKNGEIV"
     gene            complement(10813..11991)
                     /gene="pscS"
                     /locus_tag="PLI76_00590"
     CDS             complement(10813..11991)
                     /gene="pscS"
                     /locus_tag="PLI76_00590"
                     /EC_number="2.5.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-phospho-L-seryl-tRNA:Cys-tRNA synthase"
                     /protein_id="HOI60556.1"
                     /translation="MKCAVDIDARDIEELYINIDPIQAGGRLTADAMKAAISFGDGYS
                     VCDNCRNPPRLDYIRNPPIAQFHTDLAAWLNMDAVRVVPGARRGFQAVASTLVSKGDP
                     VIVTALAHYTEFMAVEAAGGIPREVPKNELNHITPDAAAEKIEAVIREFSRTPPLLFV
                     DHVDYQFGNVHDVAGIIKVAHQYDVPVLVNGAYTVGIMPVDGKALGADFVVGSGHKSM
                     AAPAPSGLLATTSEHADRVFRMTQAKGDVTGRTFGMKEVEMMGCTLMGVTVVGMMASF
                     PHVKERVKRWDVELAHSQAVTDALLSIEGTKVLSDYPRKHTLTRIDTRESFDTVAERH
                     KKRGFFLSSDLKKRGITGVIPGSTRVWKYNTFGLTDKQTRHVGESFVAVARENGLNTI
                     "
     gene            complement(11988..12170)
                     /locus_tag="PLI76_00595"
     CDS             complement(11988..12170)
                     /locus_tag="PLI76_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60557.1"
                     /translation="MSKRAVDAVFQALFILSDIRFLLRETAPQHDLDEAQKKRAATSI
                     EKVKRQIAIIEEELMR"
     gene            12301..13461
                     /locus_tag="PLI76_00600"
     CDS             12301..13461
                     /locus_tag="PLI76_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HOI60558.1"
                     /translation="MKIVHLADTHLGLSAFNRVDPETGMNLREQLIYDNFLAAVDRVI
                     AMRPDALVHAGDLFHQVKPKTRAYTTALDALSRLQDAGVPVLVVAGNHSMAKTRYTAS
                     PFEVLERGGYAARGLYVAHHNRYRRVELGDTVFHLIPNMLEAEGYRRAFDGLEFSPGT
                     NVLVTHGLASNLKDRRLHTVAEHELDATILSDRFDYIALGHFHGQIKVADNAWYSGSL
                     EYCNYGEIADTKGGLTADLSSGTVTHIDFPHTPMISLGKINCDGLSAREVMDGILGAV
                     DGAGEKTRRAICQVTLDGIRRETLRALDQKTLAEVRSRMLDLKLQVFAADDPSRIFYG
                     DSLVGLDYVAEFERFVEREHLAPGDEEYVKKTGTTILRSVIARHKEGENAAE"
     gene            13448..16621
                     /locus_tag="PLI76_00605"
     CDS             13448..16621
                     /locus_tag="PLI76_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HOI60559.1"
                     /translation="MLLNKLAMRNFKRFRNQEIAFQDGITGIVGNNGTGKSSIVAAVL
                     FALYGLQGTGLDANYIVSSFAGPQDVCEVRLDFVAGGNEYAVVRRFKRRPSSTLHEAN
                     LYLNQKLLASSVQKVGEEMQRIIGMGPGDFRNTIYAGQKELLALIESRAGSRKDWFMQ
                     VLGIDYLRKDSMEDLKRIVDAREGVSLELSGRLRELDAASVRDRLAALRADLSRAEEE
                     AKRAGAAEKNAIAALDNARGERDRLLFVRDRFLHLAREKEVLATDIARLRTECREAEE
                     EIAGRRRLQADLEALAPLAGRYEPLKAELAAMAETKAARDRLSLEAGAAGEQVREYDE
                     RRARLDAGLATLARDEQTVAGLSREVAEWQALRGRITAMKALQPEYDRLREDLARMDE
                     RFVQTERRVQENRREIETIEEKNRHLRALETEIGDYDGLRAQQEVYLRVLEYSRQEER
                     CLQEVRAGSEEMSHLEGEIAGYARQLAGMAAIEERIESAASRKEYLTSRTSACAERQE
                     TLREEIRRLAGHRADILSAGPEGACPTCRQSLGAHYEELVENLAAATASMQATLADLE
                     YEHARGTIEHQSLAAELADLQQHLTVFRRLKEQHALSSTRYEQSAGIVQRWQAEASEH
                     RAAIRALGMDGYDQAAHAALRERIRYIEGKRTAADTLRGACGRLPALQEERRRLIAEV
                     EGYLARKEDIEAKIARLGFDPEVRQRLEADAEALEASYRACTEAEARLARRPDIEEEL
                     RAVVARADGLRERLRVVTDELARLAFDPERFAHLNEECNRAEAAYRTTLELRVRLEEI
                     PRLAGALKTKQDLLASRETERLQVEKAVNDLDFDERQVAAIEKALAGCERDLFAAREQ
                     EIAIVARITGLSADIEETTGRLARADALLRQQEALTEEIGRLKLTRSLIKDYTDYLLQ
                     VVRDRIEEEAGRVLAEITDGRYSTVMLDDDFTVLVHDMGDDYPADRFSGGEQDDIAIA
                     LRVALSRFLAEVNGVHDSTFLIFDEIFGSQDEGRRNNLLRALRTQEAHFPQILLISHI
                     TEVQDEFSTTLVVEMGADQASRVREFA"
     gene            16621..17658
                     /locus_tag="PLI76_00610"
     CDS             16621..17658
                     /locus_tag="PLI76_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HOI60560.1"
                     /translation="MDQNTLYRDAIRRVAGRIREVAPPDLAERFSAAGGFDPASYRHC
                     RPEFGGAVCAVDGSNTIVLDAGCFAVAAVRASVSAYAGGARLYHRTTPLHIVTVNPGT
                     GNDDFDALFYDCFQRPPKVHLDHDDPVRNAAVIRDTLEYRAAMEMAGELDAGDLIVLD
                     GTLQVRHASHDEVVESLLNLCNLRGVLIAAVTKRTSLTWGGGHPIVPAAEGLARGLGI
                     PEPWYLSLSAAGGLLDRRETSAWKQRGEQYVARLHPRADRAFKVEIPRYYDTERVERV
                     FSALASFADDGRVTGYPYPLLDAHLTTRIGRDAVEQVRHDIIRDMDRLGMNLADYTGI
                     FGDYHDEFDRY"
     gene            17636..19234
                     /locus_tag="PLI76_00615"
     CDS             17636..19234
                     /locus_tag="PLI76_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI60561.1"
                     /translation="MMSLIDIDGDDASKYRLIGDRVLSYRFAVPHDAELYLGDILKIT
                     DSVKGLTFFAKVSDLLHDCNFSDPKWDTRPHTEHFYGIGEDVFILVEAVPLGYIGEDG
                     EFRKPRTIPAKFSRVERPESQDFAFLRQVMGDIEVGVMKTGQGVLKDVRVALHSAVMR
                     QHMGVFATTGMGKSNFMKVFCASCMRVREFGLLIVDPHGEYVAGGRSSSGESTRGLLH
                     YQAGRDGLAVFSTRSEQYRKKYHLDQLFLDYDDFRMPDLLLLYEHSPPQREVVEMLES
                     VPGSDVIDFFQGIDAATFDADTYSGPHRGIARGLKNFSASTLAVMQRRIAGMLNRNRG
                     FFRRQGTSIPDIMRALHENKVALIDIPGMSEQSELFVLSIITRMIMRNHQGEDARSAE
                     EARQVLITIEEAQRVLGSGSGSTRVFREAAMEGRKFGVGLCVVTQQPKNIDARVLAQL
                     NTFVVMGLSDRGDRDMIASSAKQDLSRLDTEIQTLEPGEAVISTIGIPFPVSTRIHLF
                     EDYIGDLSERPGARPIDDGLERTF"
     gene            complement(19317..19805)
                     /locus_tag="PLI76_00620"
     CDS             complement(19317..19805)
                     /locus_tag="PLI76_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HOI60562.1"
                     /translation="MRVTPVALAVFLFFMILIAVYVFATGDVRSLIWAVPAAVMLILI
                     PVALNYMSQKQYASLVPVYEAEARTTRVREINLNMLGETVRVKGVVERVYFQFLNRPQ
                     YLVADRTGEISVKMFTSPAEDVQKGDVVEVLGMVVKRYLLSGDAVINCVSIRKVEKVQ
                     RS"
     gene            complement(19839..20711)
                     /locus_tag="PLI76_00625"
     CDS             complement(19839..20711)
                     /locus_tag="PLI76_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HOI60563.1"
                     /translation="MVFHPPVAIVAKAGDAGKYKVGLPVWNMIVRGFMSGAYIAMGGA
                     LATVCSTGVMATDVALRSGFASAGMAQFMLGAVFPVGLIITIMTGAELFTGDAMLAPM
                     AAFIHKVSWGAVLNLWLWVYVGNLIGSVVFAYIMAYGPCVSFDAAGTATLTAFGVRAI
                     GIATAKTSYVGGMALWSVFVKGIGCNWLVNLAVLLGICADDAIGKIVGIWFPIFAFVA
                     TGFEHSVANMYFIPTGIFCTGLDPTKAVDTVNWVAMWTNNLIPVTLGNIVGGMLFVGI
                     IYWVAFRKEIAALK"
     gene            complement(21499..22764)
                     /locus_tag="PLI76_00630"
     CDS             complement(21499..22764)
                     /locus_tag="PLI76_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HOI60564.1"
                     /translation="MAGKGDLLYAWTTDEALREKAETGGAVSALLRHALTSGMVDGVF
                     AVRKGADVYDAVPALITDPADIEGIAGSLHCGTLLLPKQMRRCLLSAEPDMRIATVLK
                     GCDVKAMYEMAKRNQVNLDNILMIGLNCGGTIRPEVARTIVRERLGLDPDDVVKEEID
                     KGKFIVITKDGQHASISIDELEEGAEDLLGNEGLGRRSNCRRCKIKIPRQADLACGNW
                     GVIGERAGSATFVEVGSEKGANLLNSAVEAGAVATEAAPAKGIEIRGKVENAMLKLGD
                     RWRARYFGALGEGKERLNRIREQTNRCIKCYSCIENCPICYCIDCSTRKEYLVEPGVI
                     PPPFMFHLIRFAHISDSCVNCGQCEELCPVEIANSVFLHAIQTDLEELFGFHPGEDMT
                     PPVLALVEEKPERDRLMATGSDQIFDIFK"
     gene            complement(22768..24828)
                     /gene="fdhF"
                     /locus_tag="PLI76_00635"
     CDS             complement(22768..24828)
                     /gene="fdhF"
                     /locus_tag="PLI76_00635"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HOI60565.1"
                     /translation="MDIKYVSTTCPYCGTGCGFNLVVRNGKVFDVAHWQRAPVNGGKL
                     CPKGRYAHEFINSPDRLTKPLIKKDGKFVEATWDEAYDLIAERFGSYKPDEMACLSSA
                     RTSNEENYLMQKFARVALKTPHVDHCARLCHSSTVAGLAAVFGSGAMTNSIRDLVESK
                     CIFILGSNTFEQHPLIARSVVRARTNGAKVIVADPRYTPTARQADLYMPFVSGTDVAI
                     LNGLMQEIIRNGWEDKEFITKRTKDYEKLKEVVMKDVYSLENASKVSGIPVESLRTAA
                     EWIAAADVAALIYSMGITQHTTGVDNVKSTANLMMLTGNLGRPGGGVNPLRGQNNVQG
                     ACDMGALPNVYSGYQKVTDEATRTKMREMWGVDDIAEGKAGYTVTEMVNVLADTPGTL
                     KCMYIMGENPMLSDPDIRHVEDALRNLEFLVVQDIFLTETGRLADVILPAACYAEKDG
                     TQTNTERRVQRWRKAQDPPGDAKPDWQILCDLGKRMGYEREFAFENPEAIFAEVTAVT
                     PSYHGMSYARLDPDGLHWPCPTAEHPGTAILHREKFATPDGLGVFSAIEWKPPAEVPD
                     AEYPYVLTTGRVIWQWHTGTMTRRSWSLEKEAPIGWIEINPEDAKTLGIRDNEVVRAS
                     TRRGSIDIPARVTPEIIRGVMFIPFHYKEHPANMLTHNALDPIAKIPEFKACAVRVEK
                     VAEA"
     gene            25459..25614
                     /locus_tag="PLI76_00640"
     CDS             25459..25614
                     /locus_tag="PLI76_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60566.1"
                     /translation="MTSMSASLRTSRRLSGGRTISNRRKMRSEMQAKFPWTEANVRIS
                     SAEGTSA"
     gene            25923..27155
                     /locus_tag="PLI76_00645"
     CDS             25923..27155
                     /locus_tag="PLI76_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HOI60567.1"
                     /translation="MRLLSILIALALVAPAAAVFLTAENAETVGTGLKQVATSGNGTR
                     YEAGSYHVVVLSGTYREMGRQYGALMKDELRAEYAMLRSHFTASGYTEAEIGELARKA
                     TTLQAKRMKEIRAGIAETSGLTVEEVDILYEGVPVFFSTMNEKAFFSTAGGKAGCSFL
                     AVWGNYTPDGSVVLSRNWDHNDAFLIFKPYYTLAVYNPTDGSNSVATFGPVGLLPETL
                     MNSAGLFIAEDNGESSGSSLEIAGRPQLVGEFFRLMLDYSTMDELDAGIMSTRTDCAF
                     IVNAAGADRACSYEETVYDIKCREGTGVIAAANHFVDPSWRLAVPPTEENTVVRYGNL
                     LRLAEESKGSVDGERMVGIRDVLYDDGGATFCHYTVGDLSASSIYQVVFIPETRTLWM
                     KVVDRDWEKVELGQLFSV"
     gene            complement(27293..28108)
                     /locus_tag="PLI76_00650"
     CDS             complement(27293..28108)
                     /locus_tag="PLI76_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOI60568.1"
                     /translation="MTTVKTAAKALIMSGYGINSEMETAVALMRAGMASDTVHINDLI
                     AGEKSLSDYRLMVFPGGFSYGDDMGAGNACANRIRNNLWSDVKEFLAGDNLVLGICNG
                     FQILANLGLVPAFDEDRSREIALMPNRKGVLECRFVTLKPAAENLWTRGISHLYCPVS
                     HGEGNFFCTEETLARIERENMVAFRYCRDDLSPAGGEYPYNPNGALADIAGITSADGK
                     VLGMMPHPERAMEFVNLYDWPTRKERLKREGKPLPTESMNMRFFRNIVAYFGE"
     gene            complement(28111..31053)
                     /locus_tag="PLI76_00655"
     CDS             complement(28111..31053)
                     /locus_tag="PLI76_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOI60569.1"
                     /translation="MASHVHRIEVHYSCDPRLQMRTERFRSLGIPLRELHLVDVYTIA
                     TSKRDFSPEELSQIGAQLSNPVVQRYSVDRPTEADFDHAIEVGFLPGVTDNVGATARQ
                     TIEDYFGFQFGKGEAVFSSQLYLAAGDLTPESLEKLAGALANPLINRIHIKSREEYGK
                     KGMDAVTPFVHLDDPQAAGAVGLDLSDADLARIGKEGILDPRTGERRGPLALDLAQLH
                     AIRDYFRGLGRMPTDVEIESLAQTWSEHCKHTIFASAMDDDVPRGLYKTCIQAATNAI
                     RKKRGDADICVSVFTDNSGAIIFDDEHLVTCKVETHNSPSALDPFGGALTGIVGVNRD
                     TIGFGLGAKPCINIYGYCTGDPDTEPALFRGKNRENPILSPRRIFEGVISGVGIGGNC
                     SGIPTPQGWCYFHDRYVGKPLVFAGTVGVMPRAHNGRLLHEKQALPGDLIVMVGGRVG
                     KDGIHGATFSSEALDPASPVTAVQIGDPITQKKFSDVIVREARDLDLYRSITDNGAGG
                     LSCSVAEMAKESGGCHVLLDRVPLKYPGMAPWEIWISESQERMTLAVPEEKADAFMDL
                     MGKRDVEATVIGTFTDTGRCVVEYDGTTVMDIDLDFLHDGLPEKHLLTLPVETAYPEP
                     DFPCPDRLDDLLLAMLARKNICSKEFISTRYDHTVQGGHVLGPVQGSGRVQTAATLTK
                     VVPGSMKGVGLSQGIFPTYSELDPYRMAIAAIDLAIRGLIAIGIPLDAIALLDNFCWC
                     SSDEPERLWQLKQAAFGCRDGAIAFATPYISGKDSMYNDFSGFDADSRPVKVSVPPTL
                     LITSIGIHPDVTTAVSMDAKFEGDLAYVVGETGEELGGSEYLAHIGIEGGVVPSLDME
                     AAKARYEKMTGAIAKGLIASAFPVAHGGLLVALAKVAIAGRVGMDLTIPGGMRPDRYL
                     FSESPGRFVVTVAPGDREAFEEVFGDDAHLLGRVGGTAFRVSAGETLIEQPIRAMKTA
                     YKAPFGGY"
     gene            31316..31555
                     /locus_tag="PLI76_00660"
     CDS             31316..31555
                     /locus_tag="PLI76_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60570.1"
                     /translation="MFDRCPGAAHIRTPTLKIKKCPECGAEVEVFSNDISVRCSECGF
                     TVYNDALSCVQWCKYARECVGEETYQKLVGKKPDR"
     gene            31742..31918
                     /locus_tag="PLI76_00665"
     CDS             31742..31918
                     /locus_tag="PLI76_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60571.1"
                     /translation="MPTKTVVEGKKATPKTPPKGTLTQAEIQASIDRLDADMERWGKG
                     RRYTLAEVEKRLEL"
     gene            complement(31913..32227)
                     /locus_tag="PLI76_00670"
     CDS             complement(31913..32227)
                     /locus_tag="PLI76_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60572.1"
                     /translation="MTKYEIVITETAERHLQAIKRGDNEYIKKIIDKIKFCLGEHLFT
                     SIKQCNKKQLKGKENTHRLHIQRKYTVFYKVVKTEQKTSVEIHEVMGFEAAHKKYPHI
                     DL"
     gene            complement(32237..32395)
                     /locus_tag="PLI76_00675"
     CDS             complement(32237..32395)
                     /locus_tag="PLI76_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60573.1"
                     /translation="MKHINAKGKVFLQTSARIRENVCLLAKEERIPKSEAAEQGVIAA
                     LHARGVEA"
     gene            complement(32837..32909)
                     /locus_tag="PLI76_00680"
     tRNA            complement(32837..32909)
                     /locus_tag="PLI76_00680"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32874..32876),aa:Thr,seq:tgt)
     gene            complement(32961..33233)
                     /locus_tag="PLI76_00685"
     CDS             complement(32961..33233)
                     /locus_tag="PLI76_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF504 domain-containing protein"
                     /protein_id="HOI60574.1"
                     /translation="MRTSRRLLLRFYHDPGYDFSQVEVEYVDRGAPGDRSRVQGDRVV
                     ALDAQYLEVDAGTHAACIPYHRVRRIRYEGETMWQHGDRPGDAGKA"
     gene            33375..34187
                     /locus_tag="PLI76_00690"
     CDS             33375..34187
                     /locus_tag="PLI76_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60575.1"
                     /translation="MNLVTTIAVIVAVLLALLLAGRAGLQVQPAPFPPPAGPAAAPET
                     VPLPEGLPPPVEHFYRTLYGNDLPLIESAVISGRGTMRIGGITFPVRFRFTHETGRNY
                     HHYIENTFFGLPVMKVDEYYVNGTARLELPFGVARGSKIDQGANLALWAEAIWMPAVW
                     ATDPRTHFEPVGEHTAELVVPFGNTEERITLHFDPKTALLQEMQSMRYRGEESEEKTL
                     WLNDVREWSRMDGWLIPVTIAVAWGDEASPWAVFTAEEVVYNGEEPLRTGGV"
     gene            34282..35508
                     /gene="mmp10"
                     /locus_tag="PLI76_00695"
     CDS             34282..35508
                     /gene="mmp10"
                     /locus_tag="PLI76_00695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867903.1"
                     /note="Mmp10 (methanogenesis marker protein 10) is a
                     cobalamin-requiring radical SAM methyltransferase that
                     creates the methylarginine modification to methyl coenzyme
                     M reductase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl coenzyme M reductase-arginine
                     methyltransferase Mmp10"
                     /protein_id="HOI60576.1"
                     /translation="MSHLNVDLGGRPGLDCRGFCSYCYFKHVQGTTSFGCKYCLPFQK
                     GCDYCTRGVREGYTGFKSLRTVADETLANLQVMSDDVDRITISGGGDPSCYPEFRDLI
                     ELLGSMEVPLHIGYTSGKGFDDPGIADHLIENGLAEVSYTIFAADPDLRRRWMHDPTP
                     EASLAVLERLCGAIDAYAAAVVIPGANDGEILEATCAWLEDRGAKGLILMRFANRIDQ
                     GLILGNAPIIEGQQVHTVDEFHDLVTRLNEQFSMKISGTPLGDPSIGSPFAILHEPDL
                     LKKLPRVRGHATVITGSIAAPFIQRVLSIRGYRSRVVRVRKEIACLITAEDLAGVNLS
                     KLEDVVIIPGRAFVHDVEARRILSADGVDREVVRGPDTLTADAETSMGMTRIEVLEKE
                     MEGFAALINTINQYGR"
     gene            complement(35694..36260)
                     /locus_tag="PLI76_00700"
     CDS             complement(35694..36260)
                     /locus_tag="PLI76_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60577.1"
                     /translation="MILVIDLCYRGNSLSHAEFVGPVERYLREAGAEPIVRHYTTVSA
                     PDIEGADGAVLCGTALKDTGFLDHPDIFRWLSTFARPVLGISSGMLALAAAFGGGTVP
                     CCEIGMVDIRVVAPDPLFAGMTGFPAYELHEHAVETPTGFIPLVVSDRCIQAIRHRSL
                     PLYGLSFHPEVRNEWIIERFVPMVKRTH"
     gene            complement(36257..36937)
                     /locus_tag="PLI76_00705"
     CDS             complement(36257..36937)
                     /locus_tag="PLI76_00705"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HOI60578.1"
                     /translation="MRLGVLFSGGKDSLYACWKAMQHEEVACLITVVSKNPESYMFHT
                     PNIHLAGLQAAAAGLPLVEVETAGEQETELLDLKRALSLARERFDIEGVVTGAILSVY
                     QAARVQRICRELDLWCFNPLWHIDQEAYMEELIDAGFRVIVVGVFSSPFDASWLGREI
                     DRRTLDDLRKVARKYRTTLTGEGGEFETFVIDAPFFSRRIAIEEASAVYQNYNGILRI
                     ERAGLVEK"
     gene            complement(37101..37247)
                     /locus_tag="PLI76_00710"
     CDS             complement(37101..37247)
                     /locus_tag="PLI76_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60579.1"
                     /translation="MDDTAPRLGIPPIVYQDAINNHFDLLFLMLPDSHHSKISRCTPG
                     AHIR"
     gene            complement(37453..37537)
                     /locus_tag="PLI76_00715"
     tRNA            complement(37453..37537)
                     /locus_tag="PLI76_00715"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37499..37501),aa:Leu,seq:gag)
     gene            37899..38273
                     /locus_tag="PLI76_00720"
     CDS             37899..38273
                     /locus_tag="PLI76_00720"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOI60580.1"
                     /translation="MKAPLLVACLCCALIVTLSSSGCTTNTKDQEYYDNWVAAEPDNA
                     EAWCIRGMYYNDNYGQYAEAMESCNRALELDPGYGLAWYLKGVILTNMNMTGEAAPCF
                     ENATRYDPGLAKSVQAVAGNTR"
     gene            38611..39297
                     /locus_tag="PLI76_00725"
     CDS             38611..39297
                     /locus_tag="PLI76_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOI60581.1"
                     /translation="MYEDSYTNRLRKTALDMGADLFGVADVQNFDDPAYAGNDPRGIM
                     EDARAVIVIGVAVPRGSIESLPKGRAEYTNTLMAGTATIRIIAFRLARLIEKEGYKAT
                     IVPTEGSEFGYWYADRETLMAGMSIKYAAYCAGLGNYGANHLLITDEFGPRVRMTAIL
                     TDAPLKGPEGPRRPLLNEDCKDCMKCVEACPAAAFTADGEIHRQRCAEYMFQALGGLR
                     CGLCIKVCPK"
BASE COUNT         7366 a        12145 c        12271 g         7549 t
ORIGIN      
        1 tcgcaggaca tcgcctcggc aagcgcgacc ccgaacgact cacggtggga gagttgacag
       61 tagacctttg cgcggcggta ccagtcgggg agttcagacc gcccgacagc cccgataaac
      121 tcgacgttgg cgggagcgtc ccgtttcagg atctcaagcg caccatcaag ggcctggccg
      181 atgagaacaa accgcacctc cgggagctgc cgggctgcgt cgatgaaagt atcaagccct
      241 ttcaccctga tgttctccgt gctgacaaag cacacggtga ggacgacatc ccctttctcg
      301 ccctcaggcg aaaataccga gatatcgatg ccattgtaga tcatctcgat ccgacggggt
      361 tgggcgaccg cgaggatctc tcccttgctg aactcagaga ccgccaggat acaatcggca
      421 tgctctataa tgtagtgtac tcgtttagcc agctcaggat ccaggatcgc gccgtatccg
      481 atctcgggct catttgccac ctcatagccc cctataacaa ccaccgattt cttcccgagc
      541 agcctcgatg ccatgaccgc gaggtaagaa tggttgtggg caaaccacga gaacgttata
      601 tcggttcgga gaaccccgaa gaagatcgca agtccaagag acaacctctg caccaggctc
      661 ccggccgggc cgcccccggc gatgacctcc ctgatcggat agttctcctg gagcatgcgc
      721 aggtctcctt ctacgaagga ggaaaggctg cttgtgagga acaggatctt cggtcgatac
      781 atcgaacacc tctctacccg gcccgagcat cctggtaccc ggtgaggatc tccctgatcc
      841 ggaggctggc gtctccctcg ccaaatattc tcttctgcag gaacccaggt gaaaattgcc
      901 gaattacatc aacaattcct tcccgctccg cccccacgag cacgttccac ccggcttcga
      961 ccgtctcgac ccactcggtg ttctcccgga gggtgatgca gggcacgccg agcatgtagg
     1021 cctccttctg gaccccgccc gagtcggtga ggatcttctt cgcatgcgcc atcaggcgga
     1081 gcatatcgag gtagccgagc ggttcggtga tccggatgtt ctccggcatc gccgcgagga
     1141 gatcgtatcc cagaaggaac ttccgcgtcc gggggtggac cgggaagacc accggcaccc
     1201 cggcctcccc gagggcgccg atgatggcgg ccatgttctc ccggctatcg gtattcgacg
     1261 gccgatgaac ggtgacgacg aggtactccc ccggccggac cccggcatcc tcgaggatcc
     1321 gggagcgctc ctccgcaatc gctcggttgt ggttcatcgc gtcgaccatc acgtccccga
     1381 cgaggaagac gccctccgtg accccttcgg cggcaaggtt tcgcacagcc gtcgccgtcg
     1441 ggcagaagag gatgtccgat gcgtgatccg taagcacccg gttcacctct tcgggcatcc
     1501 ggcgatcgaa actccggagc cccgcctcga cgtgcgccac cggcacgtgc atcttcgccg
     1561 ccgcaagggc gccggcaagc gtcgagttcg tgtccccgta gactaaaacg atctcgggct
     1621 cctccttctc gaggacatcc tcgatcgccc cgagcatcgc cccggtctgg cggccgtgcg
     1681 tccccgagcc gatgccgagg ttgtagtcgg gctccgggat agcgagctcc tcgaagaaga
     1741 ccgcagacat cccgtggtcg taatgctggc ccgtgtgaac gaggatctcc tcgtgctcct
     1801 tccggagctc gcgcgagacg ggagcgcact tgatgaactg cggccgggcg ccgacgatcg
     1861 atacgatctt catggttacc gccggaccgg gtggctgttt ttatcgcccc gaccgatgcc
     1921 cttgtagacg aaccccgccc ggatgaacgc atccgggtcg acgacgtttc tgccgtcgac
     1981 gatgaccggg tgctcccgtc ccgagagctc cttcacccgc gccgggtcga gagcggtata
     2041 gtggtgatgc gccgtgaaga cgacgacgac atccgccccc gcgagcgttc ccgcaaggtc
     2101 gtgcgagacc tcaagacccg ggtagccgtt cacgaacggg tcgtgaaccc tgaccgctgc
     2161 acccgcttcc tccatcgccg cgaggtaagg ctccgccggc gtgttcctgg tgtcgtccga
     2221 gttctggatg aacgcccacc cgaggagcgc gatcgtcgtg ccctccgtgg acttccctgc
     2281 ccgcctgagc ccttcgatgg tcaggtggac catgtgccgg ggcatgaact cgttgatctt
     2341 gcgcgcgacg ccgaagatcg actccgcccc gtgcgggtac cagaggtcgc cacgcccgag
     2401 gacctgcgcg ccacgctcga ggtgccagga atccttcgtg aggcagtggc cgccgacccc
     2461 ggcacccggc cagagaatcg cccgggtgat cccctcgccc ttcaaggagt cgatccccgc
     2521 ccggacgtcg tagacattga cgcccatcgc ctcgcagtgc agtgcgagct gattcgcggc
     2581 cgcgatctgg aggtcgcgga aggcgttctc ggcggtcttc gtcacctccg ccgcggtcgc
     2641 ggtcatgggg atgatctctc ctgtcgtgag gaccggccgg tagagctcga ccgcgcgcgc
     2701 cgtgctgacg tcgtcgatcc cgccgacgat ccggtcgtgc tcgcggatgt tccggaggag
     2761 ccgcccgacc atcacccgtt cgggggcgtg agcgaggcag aagtcctcgc cggcgacgag
     2821 ccccgactcc gattcgagta tctcgcgggc catcccggca gtcgtccccg gggtaacggt
     2881 cgactcgagg accacgagcg tgccctctga gaggtgcctt cccaccgcgc ggagcccctc
     2941 gttcagggcc gagaaatcag gaatcagatc ttttgggtcg agaaacggcg tctggatggc
     3001 gagagtcacc gcatcgcacc ctctaacctc cgagaaatcc gacgtgcacc ggaacttccc
     3061 cgcgttgacg accttcccga gcaactcctc gagccccggt tcttcaccct tgagcggcga
     3121 ctcgccgcgg ttgagcatct ctatcttgta gcccgaagac gcagaggcgc gctggaaccc
     3181 gaggaccgac tcgaactccg gcgcatccgc aaagaggacc gcggcgggga taccgacgta
     3241 gcccatcccg acgacaccga tctttctgat cgggcccctg gcatcgacga tcgattgtaa
     3301 cgtattgctc ataaaaaccc tcacgccgta aaacaccgcg cgacgtcctc gcagatctcc
     3361 atattcagca gcgcttgcgc ggggctgacc gggaactccc ggttgcgcgc cacgcagtcg
     3421 aggaacgtcg agagttcgag tttcagcggc tcctgtttgt tcacgagcac cttctcgatg
     3481 atgttctcct ggacgtaacg ctcgttctct accgaatact gcccgggctt tcggtggatg
     3541 tagatctcct gggccatgaa gtccccctcg acggtgaact cctcctcctc gatgtagatc
     3601 atgcggatct tcttggatga tttcctgctt gccgagaggt agaccggggt tcccccaaaa
     3661 gagaagaggg cactgcacac atcctggttg ccgctgccgg caaggcgata tgccccgccc
     3721 gggaggagga cgtttcttat gatgtccaca tcgtggatca tcaggtcctc gacgaccgag
     3781 gagccgctca cccgggaaga ggccgggttg tgccgcttca tctcgatata gagggggtcg
     3841 cggacgatct tcttgatctc gggaacgatc gggttgaacc gctcgatgtg cccgacgccg
     3901 gcggcgaagc catccgggat cttcgcaata agcctccgcg tctcttccgc cgtcgcgcat
     3961 atgggtttct cgacgaggaa cggcaccccg gcaccgatga ccgtctctgc aaccccgaag
     4021 tgatagggcg tcgggacaca gatgctcacc acatccacag atcgaaggag atcctcgaga
     4081 gacggggagg ctgctgcctc gaaagctccg gcaaggtcgc gagtcgcctt ctcattgagg
     4141 tcgtacagat agagtgagtc taccgctttc aattccgaat agacacgggc atggtttctg
     4201 cccatcatcc cgacaccgat tacccctgca tccaaattat caccgccgac ggtattgcca
     4261 cgtatgctca tccgggaatc ccggatggta ataatactac acattggcat cgctctctca
     4321 tgacgttatc gtggagaggt atcccctcgc tccggaatgc cggggtccac aggatcacga
     4381 ccgcctctcc tgctccggtt caaaatgaat aaggttcttc acggccaaac caatgctcgt
     4441 gtttcggccc tcccggtatc ttgcccgtgt catgcgcgtg gaccctgtcc agcgccagag
     4501 cctcatcagc ttcggttcaa cgatcgggct tactgcgatc ggatttcttt ccacgatgtt
     4561 cttcacccat accgtcggtc tatctatcct gggcgcatat tttctctttg tcgcctactt
     4621 cagcgtcttc aacctgatcg gggacggcgg atttgggggc gccgcagtga agcggatcag
     4681 cgaaggagag gaccagaacg cctacttcac cgcgtttacc cttctccggg tggcgctgac
     4741 ggtcgtctcg gtgggtttcc tcctcattgc gcagccgtac ctcgtcaaac tcacctcggc
     4801 aggagtcttc ccctggctga tactcgctct ggtcgtcagc accttcacga acatcgcctc
     4861 aaacagcgtt tacgggagag gaatggtcgg cgtcaaccag atcggcagcc tgatcgacac
     4921 cctggcccgg atactcgtcc aggtcgccgc ggtcgtcctc ggctacggga ttgccggcct
     4981 cgccggcggg ttcgttgcag gcatcctggc ttcagggctc ttcaacctcc gtttcctcga
     5041 cctctccccg gccccgtttc gccggtcgca cctcaagagc ctctttgcct tctcggtctg
     5101 gacgttcctc agctcgagcg gctaccttgt cttttcgtat gccgacacca tcatgatcgg
     5161 tcacttcatg accgatgccg acgtcggcgt ctatcgcgta gcgctccagc tcacgtcgat
     5221 cgcgacgttc gtcaccgttg cactccatac caccctctat cctaaggtca gttactgggg
     5281 aaaacagggc gacctctcct cagtagagcg cgcgctcgcc cgtgctttca cctactcgct
     5341 cctgcttgcg gtcccggtcg ttgccggggg ctggctcctc ggggagcgtc tcctctactt
     5401 cttctacacg gcatcggcca ccgccggggc gggagcgctc gcgatcctcc tgctcgtcca
     5461 ggtggcccat gtcttcatgt tcctccagac gatgtgctta aacgctctcg accgaccgcg
     5521 cgactcgttc cgggtgactg cgatcgcggt tgccgcaaac gtcgggctaa acctcctcct
     5581 cataccggca tacggcatcg tcggggcgtc ggcggcgacg ctcgtcacca tgatactgaa
     5641 tgccgtactc gcccatcgcg ccctctcccg gagcatccgt atccggatag aaccccgggc
     5701 cgtgggtcat atcgtccttg ccgcactcgt catggcggcc gtcgtcgtgg tctgcacgtt
     5761 catcatcccg ctctcgaacg tctttgtcac cctcggagcg gtcggactcg gcggactggt
     5821 ctacatcctg gccctcctca ggctggatga gggtatccac gacgagctga aggatcttgc
     5881 cggacaactc ggcatcccct ggcccggtgt gctctgaggg ccggcggggc actctgccgg
     5941 ccgggaatcc cggggcgagg tgaggcggcg gtgcatccgc tcccggttgc cgggtgcccg
     6001 tctcctcccc gttccaggat gcctcctgca ggaaggcggt ccgggtaccg gtcggatagt
     6061 caggaatcga tggggcagtc gacttgaaat aataggcgcg gcaacatggg ccacacgtga
     6121 tgaagaaaga gatccgcgaa ctggagcacg aagagttccc gcttgcggag aaggtctgga
     6181 cacactaccg cgaccagaag gcggacccgc gccgggagag gatcttcggc gtctttgtcg
     6241 acggtgtcct ggcggcgacg gcccgctgca cccggcaccc gggcggcctc gagatggact
     6301 gcgtcttcac gctcgacgaa tatcgtggag gcgggtatgc gcgggaggcc gtagagatgc
     6361 tcctcgacga atgcggctcc gaaacgatct acatccactc gaccctcccc ctggtcgggt
     6421 tctacgggaa actcgggttt gtgcccatcc cggaggcgag cctgccggcg agcatcaaag
     6481 agcggttcgt cttctgcttc ggggagatgg cggggtgcaa cgtcgccccc atgatgcgca
     6541 accccacgag cgggtaaccc cgctctcctg cttaccgcgc ggcggccggc cgggggaacg
     6601 tatccgccgc cggannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccgctc
     6721 tcctgcttac cgcgcggcgg ccggccgggg gaacgtatcc gccgccggac cccgggagtc
     6781 gagcgtgccc ctcccggtca ggacgacggc aaacctgatg tgcgatgagg cccgacaggc
     6841 cggatcgttc ggtatggcaa gcatcgatta cgtcgtcctg acctggaaca gcgctgcaac
     6901 gctggaactc acgctctctt cgatccggaa ctacgggcgg ccgaaccgga tcatcgtcgt
     6961 cgataaacac tcgacggatc ggaccacggc gatcgccggg gagtacgact gtgatatcat
     7021 atcgtcgggt gcatgtctcg gggccgcaag aaggctcggg gcgcgcgagg cccgaaccga
     7081 tctcgtcgcc tttgtcgact ccgacgtgga gcttctcccg gagtgggaag aggtcctgcg
     7141 ggcctctgcc gatgggcgct accccgatgc aggggtgatc ggtgcgctct acaatgacgg
     7201 ctatctccgc ccgataaccg acccggtctc gttcctcggt ggcaacggtg cgttcggctg
     7261 ttcggtgacg cgccggcggt gcgtgctcgc atgcacggca cttgatggcc tatcgagcgg
     7321 cgaggatgcg ctttatgctg aattcctggc cgaaaagggc ctgcggtggt atatcctgcc
     7381 catctatgtc aaccatcacc gggagctcgg gaatgtccct gaggcgctga ggtggcggtg
     7441 gctgggtgcc ggccttcgcc ggcggagagg gttccgggcc tcggtgtgca aatccatcct
     7501 cggtggcgcg gtcaccggac tccgggtaaa cggtcttgac gtctcctacc gggagaacct
     7561 taccctgcgg ttgaactact tcgtcggcta catgctcccc ggaaagtact tcgaggtcga
     7621 gaggaaggag agacggccat gataggttcc cgctaatgct cgatcttctt gatatgctcg
     7681 atggcgttct ccgcgagttt ctccctcccc tgcagttcgg cgacgtcccg tatcgcctcg
     7741 agcatgtgga cggagtcttc gaggaaactc aggatatcgg cagggtagag gtcgatcccg
     7801 tattcgtcga ggagatgggc gccgatctgg cgatgatcga gccccatctc ccggaactcg
     7861 atgatggcga gggtgaactt ccgctccggg cacccgcaga agggggcgtt tttacacttg
     7921 cagtccatga aatcgcggaa aaaagcaagg agctgctcct gcatccggcg gtcgaggtgc
     7981 tcgaagttga tcgccttccc gagcgattcg aggaacgcgc ccgagaagac atggtccggc
     8041 agccggaatc ctgccgcccg ctccagcttc cgcgccgccc ggaaccgggc cttatccgcg
     8101 atcacgacgc ctcgccgggc tcctcgtcga acttcgcgag agacttcatg gcgttgctca
     8161 tggtctcgat ctcgatgagc cattcgtcga agaggccggg gttctccgca tcgtccttga
     8221 tgtacttcgc gcaacaggtg gcgccatcga caacggcggc gccgatattt gccgcaacct
     8281 caagagcttc ctcgaagttc tcctcatcga tgccccggcc tttcttcacg agcgccttga
     8341 tgtctttatc gaattcgccc tcgatatact tccggcaggc ggtgaagagt acgagcaggg
     8401 agagctggag cgacccgatg atatcctcga gttctccctc gggaagcccc gtcatgacga
     8461 tcagttccac gtcgtcgagc ttcgcgatgg cctcctcctt cgtgaaccgg tcgttctggt
     8521 agaggcggat gatcttcaag accgagaggg tgatatcttc ggagaaactg tagagcatct
     8581 gctcgccttc ggagacgtct tcgccttcgg cgggctcgaa gtcagcaccc tccagtgtct
     8641 tgatccagtt gtcccagcgc tcctgattgt aaaaaatata gaagagtttc atgggttctg
     8701 cctgtttggc tttcgctttc ttcgccatat cagtacacat tcggagtgcc tcttttaaaa
     8761 atcatttgct ctctgtgccg aaagcccgaa tcgccgcgat tcgcgaacct tcatgtaggc
     8821 ccggcgagca taagctgagc agatgacaca cgatgcggat ctcgcgctgc tcctgaaatc
     8881 ctgcgaaccc attgcgatcg ccctctccgg gggaacagac agttcggtcc tgctggccta
     8941 tgcccggcgg cacggcatcc gggcgatcgc tatcagcgtc gataccggcc ttaccccccc
     9001 gggggaactc gaggcggcgc gggttctctc ggagcgtctc ggcatctcgc acatcgttat
     9061 accgctcgat gcgctcgata tccctgcggt ccgggagaac cggccggacc ggtgctacgc
     9121 ctgcaagagg gcgatgatgg aggcggtcgt ccgcgaggca gagcggcgcg gatgccgggc
     9181 ggtggtcgac gggacccacg ccgacgaccg atccgccgca aggcccggta tgcgggcact
     9241 cgccgaactc gggataagga gcccgtttgc ggagtgcgac ataggaaaga aggatatcga
     9301 ggcccttgcg gcagacctcg gggtgcccgt ccgcccaccg tccgcatgtc ttgcgacgcg
     9361 catccccgcc ggcgatgcgg tcacccgtga gtgcctcgcg ctcgtcgcgg ctgcggaagc
     9421 tctcatcgcg aaagagatcc ccgggaccat acgggtccgg tgcatcgggg agggcgcccg
     9481 ggcctccatc gaggccgatc ccgcatacca tgataggctg gaagaactgc tcgatgcggt
     9541 aaggaacctc gggtttgagg atgcggtcat cgcctccgga ggctaccgcg agggaggtgc
     9601 ggacgcatgg aagcggtgat gatcaggtac ggcgagatat tcctcaagag cgagacggtg
     9661 aaacggcggt tcatatccat catgacggag aacatccggc tcgcgctgga ggcaaaaggg
     9721 ctctcccacc gcatcgaaac gccccggggg cggatcctga tcttcggcga cgaaccccgg
     9781 cgcatcgccg cggccgtcgc cggaaccttc ggggtggtga gcgcgagcgt ctgcacgatg
     9841 acctcttccg atatcgatac tatcgcggcc gtagccgtag agcacgcagg acggcacctc
     9901 cggccgggga tgtcgtttgc ggtcagggca cgccggtcgg gcgtcgaggg gttcaacagc
     9961 caggaacttg cggcggcggt cggctcggcc atcctcgacc gggtgccgga ggccacggtg
    10021 gacctctcga cgcccgatta cgaggtcttt gtggaggccc gggagttcgg cggcctcgtc
    10081 tacgacgaga ggctcaaggg gccgggaggg ctcccttacg ggacgcaggg ggaggtgatg
    10141 gccctgctct cggaggggat cgactcgccc gttgcatcgt ggctgatgat gcgccggggg
    10201 tgccgggtgg tgcacgtcca catgcacggc ggacggttcg gcggagcgga cggagaagag
    10261 acggttctcc ggcaccacgc ccggctctcg gcctgggttc cggggcatcc tctcgacctc
    10321 ctcgtcgtgg acatggagcc gttctttgag gtcatcatcg ccttaaaaga gccccgttac
    10381 cggtgtatcc tctgcaagcg gttcatgctc agggtggcag gcatccttgc agaagaacgc
    10441 ggcgcctacg cgctcgtcaa cggcgacaac ctggggcagg tggcgtccca gacgctcgcg
    10501 aacatggcgg ttatcgcccc ggcggcatcg gtgacggtgc tccggccgct gatcgggttt
    10561 gataaggagg agatcgtcga acgcgcccgc gccatcggga cgtttgaggc ccgggcgggg
    10621 agcaccgggt gcacggtcgc cccccgttac ccctcgacgg cggcacctgc agcgacgatc
    10681 gtcgatatcg aggagaagat cggcgcgaaa gacctcgcgg cccgggcggc ggggacggtg
    10741 cgggtatatc gcgcgaagaa cggggagatt gtgtaagaat tttttccacc cgaaaaaaaa
    10801 gattcgggat cttcagatag tattcaaccc attctcccgg gcaaccgcga caaacgactc
    10861 cccgacgtgc cgggtctgct tatcggtcag cccgaaggtg ttgtacttcc agacccgggt
    10921 cgagccgggg atgacgccgg tgatcccgcg cttcttcagg tcgcttgaga ggaagaagcc
    10981 gcgtttttta tgccgctcgg ccaccgtgtc gaaggattcg cgggtgtcta tacgggtcag
    11041 ggtgtgcttt ctcgggtaat cggagaggac tttcgtcccc tcaatggaga ggagggcatc
    11101 cgtgaccgcc tgtgaatgcg caagctcgac atcccagcgc ttcacccgct ctttgacatg
    11161 ggggaaggag gccatcatac cgacgacggt gacgcccatc agggtgcagc ccatcatctc
    11221 cacctccttc atgccgaacg tcctgccggt cacgtcccct ttcgcctgtg tcatccgaaa
    11281 gactcgatcc gcgtgctcgc tcgtcgtcgc gaggagcccc gagggcgccg gggcggccat
    11341 gctcttgtgg cccgacccga cgacgaagtc ggcgccgagc gctttgccgt cgacgggcat
    11401 gatcccgacc gtgtaggctc cgttgacgag gaccgggacg tcgtactggt gggcgacctt
    11461 tatgatcccc gcgacgtcgt gaacgtttcc gaactggtag tcgacgtggt cgacgaagag
    11521 cagcgggggt gtgcgcgaga actcacggat caccgcctcg atcttctctg cagcggcgtc
    11581 aggggtgata tgattcaact cattcttcgg gacctcgcgc gggatcccgc ccgccgcctc
    11641 gaccgccatg aactccgtat agtgggcaag cgccgtcacg atgaccgggt cgcccttcga
    11701 gacaagcgtg gaggcgactg cctggaaccc ccggcgcgct cccggcacca cccgcaccgc
    11761 atccatgttc agccatgccg cgaggtccgt gtggaactgc gcgatgggag ggttccggat
    11821 gtagtcgagc cggggagggt tccgacagtt atcgcagacc gagtagccgt cgccgaacga
    11881 tattgccgcc ttcatggcat cggcggtgag ccgcccgccc gcctggatgg gatcgatgtt
    11941 gatgtacagc tcctcgatgt cgcgggcatc gatatcgact gcgcacttca ccgcatcagc
    12001 tcctcttcga tgatggcaat ctgccgcttc accttctcga tggaggttgc ggcacgcttc
    12061 ttctgcgctt catcgaggtc atgctgcgga gcggtctccc ggagcaggaa ccggatgtcg
    12121 gagaggatga agagtgcctg aaaaactgca tcaacggctc tttttgacac gagatcacca
    12181 tacaaatatc attgagcata tattaggtta cgcgtattcc gggcatcggc ccggcatcga
    12241 acactttcac accgcacgca gcagccattt atacccgcaa cacacggaga ttaattgggc
    12301 atgaagatcg ttcatttagc agacacgcat ctcgggttgt cggcgttcaa ccgggtcgat
    12361 ccggaaaccg ggatgaacct gcgcgaacag ctcatctacg acaacttcct cgccgccgtc
    12421 gatcgggtca tcgccatgcg ccccgatgcg ctcgtccatg cgggcgacct cttccaccag
    12481 gtcaaaccga agacccgcgc ctacacgacg gcgctcgacg ccctctcccg cctgcaggat
    12541 gcgggagttc cggtgctcgt ggtcgccggc aaccacagca tggcaaagac ccgttacacg
    12601 gcgtccccgt tcgaggtgct cgagcggggc ggctacgctg cacgcggtct ctacgtcgcc
    12661 caccacaacc gttaccggcg ggtggaacta ggagatacgg tctttcacct cataccgaac
    12721 atgctcgagg cggaaggtta ccggagggcg tttgatggac tcgagttctc gcccggcacg
    12781 aacgtcctcg tcacccacgg ccttgcgagc aacttgaaag accggcggtt gcacaccgtc
    12841 gccgagcacg aactggatgc gacgatcctc tccgaccggt tcgactacat agcgctcggc
    12901 cacttccacg gccagataaa ggtcgccgac aacgcctggt acagcgggtc gctcgaatac
    12961 tgtaactacg gcgagatcgc agataccaaa ggaggcctta ccgcggacct ctcgtccgga
    13021 acggtcacgc atatcgactt cccgcacacc cccatgatca gcctcggtaa gatcaactgc
    13081 gacggcctct ccgcccgcga ggtcatggat gggatcctcg gcgcggttga cggcgcaggg
    13141 gagaagaccc gccgggcgat ctgccaggtc acgcttgacg ggatacgccg cgagacgctc
    13201 cgcgcgctcg accagaaaac cctcgcggag gtccggagcc ggatgctcga cctcaaactg
    13261 caggtgttcg ccgccgacga tccctcccgt atcttttatg gagactcgct cgtcggcctc
    13321 gactacgtcg ccgagttcga gcgtttcgtc gagagagagc acctcgcccc gggcgacgag
    13381 gagtacgtga agaagaccgg gacgacgatc ctccgctcgg tcatcgcgcg gcataaggag
    13441 ggagagaatg ctgctgaata aacttgccat gcgcaacttc aagcggttcc gcaaccagga
    13501 gatcgccttc caggacggca tcaccgggat cgtcgggaac aacgggaccg gcaagagcag
    13561 catcgtcgcc gccgtcctct ttgccctcta cggcctccag gggacggggc ttgacgcgaa
    13621 ctacatcgtc agttcctttg cgggcccgca ggacgtctgc gaggtccgcc tggacttcgt
    13681 ggccggcggc aacgaatacg ccgtcgtccg caggttcaag cgccgcccct cctcgaccct
    13741 ccacgaggca aacctctacc tgaaccagaa gctcctcgca agcagcgtcc agaaggtggg
    13801 ggaggagatg cagcggatca tcggcatggg tccgggtgac ttccgaaaca ccatctatgc
    13861 cgggcagaag gagctcctcg ccctgatcga gagcagggcc ggatcccgga aagactggtt
    13921 catgcaggtg ctcggcatcg attacctcag gaaggacagc atggaggacc tgaagcggat
    13981 cgtcgatgcc cgggaagggg tctctctgga gctctccggg cgcctccggg agctcgacgc
    14041 cgcctccgtg cgcgaccgcc ttgcggcgct ccgcgccgac ctctcccgtg cggaggagga
    14101 ggcaaagcgt gccggcgccg cagagaaaaa cgccatcgcg gcgctcgata acgcccgggg
    14161 ggagcgcgac cggctgctct ttgtgcgcga ccgtttcctg caccttgcgc gggaaaaaga
    14221 ggtgctcgcg accgatatcg cgcgactccg caccgagtgc cgggaggccg aggaagagat
    14281 cgcaggacgc aggagactgc aggccgacct cgaagccctt gccccgcttg ccgggcgcta
    14341 cgaacccctg aaggcggagc ttgccgcgat ggcggagacg aaggctgcac gcgaccgcct
    14401 gagcctcgag gccggcgccg cgggcgagca ggtgcgggag tacgacgaac gtcgcgcgcg
    14461 cctcgatgcc gggctcgcca cgcttgcccg ggacgaacag acagttgccg ggctttcccg
    14521 ggaggtcgcg gagtggcagg ccctccgcgg tcgtatcacg gcgatgaagg cccttcagcc
    14581 ggaatacgat cgtctccgcg aagaccttgc ccgcatggac gagcggttcg tccagacaga
    14641 gcgccgtgtc caggagaacc ggagggagat cgagacgatc gaggagaaga accggcatct
    14701 tcgtgcgctc gagaccgaga tcggcgacta cgatggtctc cgggcccagc aggaggtgta
    14761 tctcagggtt cttgagtact cccggcagga agagcggtgc ctgcaggagg tccgcgccgg
    14821 gtccgaggag atgagccatc ttgagggcga gatcgccggg tatgcccggc aactcgccgg
    14881 gatggccgcc atcgaggagc ggatcgagtc ggcggcaagc cggaaggagt acctgacttc
    14941 acgaaccagc gcatgcgccg aacgccagga aacgcttcgc gaggagatcc ggcggctcgc
    15001 cgggcaccgg gcggatatcc tctctgcggg cccggaagga gcctgcccga cgtgccgcca
    15061 gagcctcggc gctcactacg aggagctggt cgagaacctc gccgccgcca ctgcatcgat
    15121 gcaggcgacg cttgcagacc tcgagtacga gcatgcgcgg gggaccatcg aacaccagtc
    15181 cctcgccgcc gagcttgccg acctccagca gcacctcacc gtcttccggc gcttgaaaga
    15241 gcagcacgcc ctctcctcga cccggtacga gcagagcgca ggtatcgtgc agcgctggca
    15301 ggccgaggca agcgagcacc gtgccgcaat ccgggcgctc ggcatggacg ggtacgacca
    15361 ggctgcgcac gccgccctca gggagaggat ccggtatatc gaggggaagc ggacggcggc
    15421 cgataccctc cggggcgcgt gcggccgcct ccccgccctc caggaggagc ggcgacggct
    15481 catcgccgag gtcgagggtt acctcgcccg caaggaggat atcgaggcga agatcgcccg
    15541 gctcgggttc gacccggagg tccggcagcg tctcgaggcg gatgcggagg cgcttgaagc
    15601 atcctatcgg gcatgcacgg aagcagaggc ccgccttgcg cggagaccgg acatagagga
    15661 ggagttgaga gccgtcgttg ccagggccga cgggctccgc gagaggttgc gggtcgtcac
    15721 ggacgaactc gcccgcctcg cgttcgaccc ggagaggttc gcgcacctca acgaagagtg
    15781 caaccgtgcc gaggcggcgt ataggacgac cctggagctc cgcgtccggc tcgaagagat
    15841 cccgcggctc gccggggcgc tcaagacaaa gcaggacctc ctcgcgagcc gggagaccga
    15901 acggctccag gtcgagaagg ccgtaaacga ccttgacttc gacgagcggc aggtcgcggc
    15961 gatagagaag gcgctcgcag gatgcgaaag ggatctcttc gccgcacggg aacaggagat
    16021 cgcgatcgtt gcccgcataa ccggcctctc ggccgatatc gaggagacaa ccgggaggct
    16081 tgcccgtgcg gatgccctcc tccggcagca ggaggcgctc accgaggaga tcgggcgcct
    16141 gaaactcacc cgatccctca tcaaagatta caccgactac ctcctccagg tggtcaggga
    16201 ccggatcgag gaggaggccg ggagggtgct tgcggagatc accgacggga ggtacagcac
    16261 cgtgatgctc gacgacgact tcaccgtcct cgtccacgat atgggggacg actacccggc
    16321 cgaccggttc agcgggggag agcaggacga tatagcgatc gccctccgcg tcgccctctc
    16381 ccggttcctt gccgaggtga acggggtgca cgactccacg ttccttatat tcgacgagat
    16441 attcgggagc caggacgagg gacggcggaa caaccttctc cgcgctctca ggacccagga
    16501 ggcccatttc ccccagatcc tcctcatatc tcacatcacc gaggtccagg acgagttctc
    16561 gacgacgctc gtggtcgaga tgggggcaga tcaggcaagc cgggtccggg agtttgcgtg
    16621 atggaccaga acaccctcta ccgggacgcc atccggcggg tcgcaggaag gatccgtgaa
    16681 gtcgccccgc cggatcttgc agagcggttc tcggccgccg gcgggtttga ccccgcctcc
    16741 taccggcact gccggcccga gttcggcggc gccgtctgcg ccgtcgacgg gagcaacacc
    16801 atcgtcctcg acgcgggatg ttttgccgtc gccgccgtcc gggcatcggt cagcgcgtat
    16861 gccggtggtg cgcgcctcta tcacaggacg acgcctctcc atatcgtcac ggtcaacccc
    16921 gggaccggga acgacgactt cgatgcgctc ttttacgact gcttccagcg tcccccgaaa
    16981 gtgcatctcg atcacgacga ccccgtgagg aacgccgccg tcatacggga taccctcgag
    17041 taccgggcgg cgatggagat ggccggagaa ctcgatgccg gcgacctcat cgtcctcgac
    17101 ggcacgctcc aggtccgcca cgcaagccac gacgaggtcg tcgagagcct cctgaacctc
    17161 tgcaacctcc ggggggtgct catcgccgcc gtgacgaaac ggacgtcgct cacctggggc
    17221 ggggggcacc cgatcgtccc ggcggcggag gggcttgccc gcggcctcgg catccccgag
    17281 ccctggtatc tctctctctc cgccgccggg ggcctgctcg accgccggga gacctccgcc
    17341 tggaagcagc ggggcgaaca gtacgtcgcg cggctgcacc ctcgggccga tcgggcgttc
    17401 aaagtggaga tcccccggta ttacgacacg gagcgggtag aacgggtctt ctccgcgctc
    17461 gcctcatttg ccgacgacgg aagggtcacg ggttacccgt atccgctcct tgacgcccac
    17521 ctcaccacac ggatagggag ggatgcggtc gagcaggtcc gccacgatat catccgcgat
    17581 atggaccggc tcggcatgaa cctcgccgat tacaccggca tcttcggtga ttaccatgat
    17641 gagtttgatc gatattgacg gcgacgacgc ctcgaagtac cgcctgatcg gagaccgggt
    17701 gctcagctac cggtttgccg ttccccacga tgcggagctc taccttgggg atatcttgaa
    17761 gatcaccgac agcgtcaaag ggctgacgtt ctttgccaaa gtcagcgatc tcctccacga
    17821 ctgcaacttc tccgacccga agtgggacac ccgcccgcat accgagcact tttacggtat
    17881 aggggaggac gtcttcatcc tcgtcgaggc ggtccctctc ggttacatcg gggaggatgg
    17941 cgaattccga aaaccgcgga cgatcccggc aaagttctcc cgcgtggagc ggccggagtc
    18001 gcaggacttc gcgttcctcc ggcaggtgat gggcgatatc gaggtcggcg tgatgaagac
    18061 cggccagggt gtcctgaagg atgtaagggt ggcgctccac tcggcggtga tgcggcagca
    18121 catgggtgtc tttgcgacga cggggatggg aaagagcaac ttcatgaagg tcttctgcgc
    18181 ctcctgcatg cgggtgcggg agttcgggct cctcatcgtc gacccgcacg gcgagtacgt
    18241 ggccggaggg cgatcgtcga gcggagagtc gacgagaggg ctcctgcact atcaggcggg
    18301 ccgtgacggc cttgccgtct tctccacccg gtcggagcag taccggaaga agtatcacct
    18361 ggaccagctc tttctcgact acgacgactt tcgaatgccc gacctcctcc tcctctacga
    18421 gcattctcca ccccagcgcg aggtcgtgga gatgctcgag agcgtgccgg gctcagacgt
    18481 gatcgacttc ttccagggga tcgatgccgc gaccttcgat gccgatacct acagcggccc
    18541 gcaccgcggg attgcccggg gcctgaaaaa cttctccgca agcacgcttg ccgtcatgca
    18601 gcggcgcatc gcgggcatgc tcaaccggaa ccggggtttc ttccggagac agggcacctc
    18661 gataccggat atcatgaggg cgcttcacga gaacaaagtc gccctgatcg atatacccgg
    18721 tatgagcgag cagagcgaac tcttcgtcct ctcgatcata acccggatga tcatgcgcaa
    18781 ccaccagggc gaagatgcaa ggagcgcaga agaggcgagg caggtgctga tcaccatcga
    18841 ggaggcgcaa agggtgctcg ggtcggggtc cgggagcacc cgggtcttcc gggaggccgc
    18901 gatggagggg aggaagttcg gtgtcggtct ctgtgtggtt acccagcagc caaagaacat
    18961 cgacgcccgg gtccttgcgc aactgaatac gttcgtcgtg atggggcttt ccgaccgggg
    19021 ggaccgcgac atgatagcga gcagcgcaaa acaggacctc tctcgcctgg ataccgagat
    19081 ccagacgctt gagccgggag aagcggtcat cagcaccatc ggtataccgt ttcccgtgag
    19141 cacccggatc catctcttcg aggactacat cggagacctc agcgaaaggc cgggggcaag
    19201 accgatagac gacggccttg agagaacatt ttagcaggaa tgcaagaagg tgatggcggc
    19261 agggttctat cccgtgtcaa ccctcccttc actgaacact ctggcgccct gaggcgtcag
    19321 gatcgctgaa ctttctcgac tttccggatg gagacgcagt tgatgaccgc atccccggac
    19381 aggaggtagc gtttgacgac cattcccagt acctcgacca catccccttt ctgaacatct
    19441 tccgccgggg aggtgaacat cttgaccgat atctcacccg tccggtcggc gacgagatac
    19501 tgcggccggt tgaggaactg gaagtatacc cgctcgacga cgcctttgac gcgcaccgtc
    19561 tcgccgagca tgtttaagtt gatctcgcgg acgcgggttg ttcgtgcttc tgcttcatag
    19621 accggcacca gcgatgcata ctgtttctgg ctcatgtagt tgagcgccac tggtatgagg
    19681 atcagcatga ctgctgccgg aaccgcccag atcagcgaac gtacatctcc ggtagcaaag
    19741 acgtacaccg caatcaggat cataaaaaag aggaagacgg ccagtgcaac tggcgtcacc
    19801 ctcactctaa tatctccaat ctgcattgtc aatcaggttt acttgagggc tgcaatttcc
    19861 tttctgaacg cgacccagta gatgatacca acaaagagca taccgccgac gatgttgcca
    19921 agcgtgactg gaatcaggtt gttggtccac atggctaccc agttgactgt gtcgactgcc
    19981 tttgtggggt caagacctgt acagaagatg cccgtgggaa tgaaatacat gtttgcaaca
    20041 ctgtgttcaa acccggtggc gacgaaagcg aagatcggga accagatacc gacgatcttg
    20101 cctatcgcat cgtcagcgca tataccgagc aggacagcaa ggttgacgag ccagttacaa
    20161 ccaataccct tgacgaatac cgaccataga gccataccgc cgacgtaact cgtctttgcc
    20221 gttgctatgc cgatcgctct cacaccgaat gcggtgagtg ttgcggttcc ggcggcatcg
    20281 aaggagacac acgggccgta tgccatgata tacgcaaaga ctaccgaccc gatgaggttg
    20341 ccgacataca cccagagcca gagattgagc acagcgcccc agctgacctt gtggatgaac
    20401 gctgccatgg gggcgagcat cgcgtcgccg gtgaagagtt ctgcaccggt catgatcgtg
    20461 atgataagcc ccacagggaa gacggcaccc agcatgaact gggccatacc ggcgcttgcg
    20521 aaaccggatc tcagtgcgac gtcggttgcc atgacgccgg tcgaacagac tgttgcgagc
    20581 gcaccgccca tggcgatgta ggctccagac atgaagccac gcacgatcat gttccagacg
    20641 ggcagcccga ccttgtactt tccggcatca cccgcttttg ctacgatagc aactggagga
    20701 tggaacacca tttttcatac acctctctaa acgtcctacc tataccatca agtaatcaga
    20761 ctgtataggt ctaattaatc agtaatttga gagggatatt aataagtttc gaattgatcg
    20821 tcacgcagga agagtataac tcaaatttag gcgtttatca tcgggttgag ttaattcccg
    20881 taattaaaca tcaagaatcg ataaacgacg cttctggatt tgtttttgag accacgcgga
    20941 atataggtag ttttagggtg atgtttgagc gagcgtgcgg gagcaggcgc gcagctcctc
    21001 caacctcctg ctcttcctct cgtggggcga tgatcgccca ctaaaatcgt cgcggatgcc
    21061 tgcgcgtcgc cggatgccac cacgacccgg gtgtgatacc cgggaattac tctttttttg
    21121 gtatattcag gccgtacctc tcgtttttcg agcgggcttg gagccctgga gcgaggcctg
    21181 atccgggaga ttcagggaaa ctggagcgtc ttgcctgcgg ccccccacca ggtccgtcac
    21241 ccggtgccag gggatccttc gccacgagcc ctcgtcccgg cgctacatcc cgctgagacg
    21301 cacacacctt tcttctggca ccacaacgcc atgctgacaa tccgaaacac cagcccggga
    21361 agatcatgag ctataggtgc gctgtaaggc atcttggggg aacccggggg tgggtcgcac
    21421 ggggaaaccc gggagattca ctttcccggg ttccaactca tctgaccatc ccccggactc
    21481 ctatcctgcg ggcaggggtt acttgaagat gtcgaatatc tggtcgctgc cggtggccat
    21541 gagccggtcg cgctcaggtt tctcctcgac gagcgcgagc accggcgggg tcatatcctc
    21601 gcccgggtgg aacccgaaga gttcctcgag atcggtctgg atggcgtgga ggaagaccga
    21661 gttcgctatc tcgacggggc agagctcctc gcactggccg cagttgacac aggagtcgga
    21721 tatgtgcgcg aaccggatca ggtggaacat gaacggcggc gggatcacgc ccggctcgac
    21781 caggtactcc ttccgtgtgc tgcaatcgat gcagtagcag atcgggcagt tctcgatgca
    21841 ggagtaacac ttgatgcacc ggttcgtctg ctcccggatc ctgttcaggc gctccttacc
    21901 ctcgccgagc gccccgaagt agcgcgcccg ccaccggtcg ccgagcttca gcatcgcgtt
    21961 ctcgaccttg ccacggatct cgataccctt tgccggcgcc gcctccgtcg ccaccgcgcc
    22021 ggcctcgaca gcgctgttga ggaggtttgc gcccttctcg gagccgacct cgacaaacgt
    22081 ggcactgccg gcgcgctcac cgatgacccc ccagttgccg caggcgaggt ccgcctggcg
    22141 cgggatcttg atcttgcacc ggcggcagtt ggaccggcgg ccgagcccct cgttcccgag
    22201 gaggtcttct gccccctcct cgagttcgtc gatcgagatg gatgcgtgct ggccgtcctt
    22261 cgtgatgacg atgaacttac ccttgtcgat ctcctccttg acgacatcgt cggggtcaag
    22321 gccgagtctc tcccggacga tcgtccgggc gacttccggc cggatggtgc cgccgcagtt
    22381 gaggccaatc atcaggatgt tatccagatt gacctggttg cgcttcgcca tctcgtacat
    22441 cgctttgacg tcgcaaccct tgaggaccgt cgcgatacgc atatccggct cggcggagag
    22501 aagacaacgg cgcatctgtt tcgggaggag gagggtgccg cagtggagcg agcctgcaat
    22561 cccctcgata tcggcggggt cggtgatcag cgccggcacc gcatcataga catccgcgcc
    22621 cttcctgacc gcaaagaccc cgtcgaccat accgctcgta agagcgtggc gcaacagtgc
    22681 gctgaccgct ccgccggtct ccgccttctc cctgagcgcc tcatccgtcg tccatgcata
    22741 gagcaaatcg ccttttcctg ccatatctca cgcctccgca accttttcga ccctgaccgc
    22801 acacgccttg aactcgggga tcttcgcgat cgggtcgagt gcgttgtggg tcaacatatt
    22861 cgccgggtgc tccttgtagt ggaacgggat gaacatgacg cccctgatga tctccggggt
    22921 gaccctggcg gggatatcga tggacccgcg gcgggtgctt gcccggacga cctcgttgtc
    22981 cctgatgccg agcgtctttg catcctcggg attgatctcg atccagccga tcggcgcctc
    23041 tttctcaagg ctccacgacc ggcgagtcat cgtgccggtg tgccactgcc agatgacccg
    23101 cccggtcgtg aggacgtacg gatactcggc gtcggggacc tcggcgggag gtttccattc
    23161 gatagcggag aagaccccga ggccgtccgg cgtggcgaac ttctcccggt ggaggatggc
    23221 cgttcccggg tgctccgcgg tcggacaggg ccagtgcagg ccgtccgggt cgaggcgggc
    23281 gtaggacatg ccgtggtaag acggggtgac ggcggtgact tcggcaaaga tcgcctcagg
    23341 gttctcgaac gcgaattctc tctcgtaacc catcctcttc ccgaggtcgc agaggatctg
    23401 ccagtcgggt ttcgcatccc ccggtggatc ctgggccttc ctccagcgct ggacccgccg
    23461 ctcggtgttc gtctgggtgc cgtctttctc cgcgtagcag gcggcgggca ggatcacatc
    23521 ggcaagacgg ccggtctcgg tcaggaatat gtcctgaacg accaggaact cgaggttcct
    23581 gagcgcatcc tcgacgtgcc ggatgtccgg gtcggagagc atcgggttct cgcccatgat
    23641 gtacatgcac ttgagcgtgc cgggcgtatc ggcaaggacg ttcaccatct cggtgacggt
    23701 gtaaccggcc ttaccctcgg cgatatcgtc gacgccccac atctccctca tcttcgttct
    23761 ggtcgcctcg tcggtgacct tctggtagcc ggagtagacg ttcgggaggg ctcccatgtc
    23821 gcaggcgccc tggacgttgt tctggccgcg aagcgggttc acgccgccgc cgggcctgcc
    23881 caggttgccg gtgagcatca tgaggttcgc ggtcgatttg acgttgtcga cgccggtggt
    23941 gtgctgggtg atgcccatcg agtagatgag cgcggcgacg tcggcggctg cgatccactc
    24001 tgccgcggtc ctgaggctct cgacgggaat accggagacc ttcgaggcgt tctcaaggct
    24061 gtagacatcc ttcatgacga cctccttgag tttctcataa tccttcgtcc gcttcgtgat
    24121 gaactccttg tcctcccaac cgttcctgat gatctcctgc atcagcccgt tcaagatggc
    24181 gacgtcggtc ccggagacga acggcatgta gaggtcggcc tgccgggcgg tcggcgtgta
    24241 gcgcgggtcg gccacgatca ccttcgcacc gttcgttctc gcgcggacga cgctccgcgc
    24301 gatcaggggg tgctgctcga atgtattgga cccgagtatg aagatgcact tcgactcgac
    24361 gagatcccgg atcgagttcg tcatcgcccc ggacccgaag accgccgcga ggccggcgac
    24421 ggtggacgag tggcagagcc gggcgcagtg gtcgacgtgc ggcgtcttga gcgccacccg
    24481 ggcgaacttc tgcatcaggt agttctcttc gttcgatgtc cgggcggagg agaggcaggc
    24541 catctcgtcg ggtttgtagg atccgaatcg ttccgcgatc aggtcgtagg cctcgtccca
    24601 ggtcgcctcg acaaacttgc cgtccttctt gatgagcggt tttgtcaggc ggtcggggct
    24661 gttgatgaat tcgtgggcgt aacgtccttt ggggcagagt ttacccccgt tgacgggtgc
    24721 gcgctgccag tgcgccacgt caaagacctt gccgttccgg acgacgaggt tgaacccgca
    24781 cccggtgccg cagtacgggc atgttgtcga tacatacttg atatccattg tcgttcccct
    24841 ctggcaggga gaacataccg cccgtgtcat ataataggca tggtccaacg tggttttcga
    24901 ggtaaattaa cgatatacgg caaatacacg gtatatgtcc tgcccggggt caccccgaaa
    24961 cggcaggatg ccgctccacg tggtacgggc cggcgtcggt cccgttgttc ttgcccgcgt
    25021 gcttcggtga tccgggcctt tgcgatctcc cggtagccag gatcgatctc aaacgtcccg
    25081 gggtttgccg ctgtcgtcgg cgaggacccg cgccgctcca gtggcgtctt cgcccatcca
    25141 gccgaggtaa gtcatcccgg gactttttgt cgtcgtacgg gttgtagtcc ttccctatgg
    25201 cgtagaacgg cgacgtgacg atgatgcctc cgggaggcgc cgcgtgcctt cgatgcagtc
    25261 cacggtaagc ccgcctgctc ttctctcttc ccccctcact ccaatcacgt atcgatccgg
    25321 ggcgaaagag taaacaaaac tcgatctcag gcaccatcgc ctccggtgag aaccttctgc
    25381 cgcgttcgct cgagcgcatc gacgaagtcc tcgacctcgt agggattgag ctccccctgc
    25441 ccctcaggtt cgaggcgtat gacctcgatg agcgcatcct tgaggacgtc gaggcggctg
    25501 tcgggaggga ggacgatctc gaaccggcgg aagatgcggt cggagatgca ggcgaagttt
    25561 ccgtggacgg aggcaaacgt caggatctcg tctgcggaag ggacgtcggc ctgatcgacc
    25621 tcgacttcga ggactgccgg cgtatcggcg cttttctcta taaagcagcg ggcgaccctg
    25681 atcttcgtgg cgactcctcc gttcagttcc ggatattctc tcggaagagt gatatagttt
    25741 tatgccgggg cgcgggaggt cttctttgca gaccggatgc cgtccgggtc ttggccggca
    25801 tcccgatccg tctgccggga gatgcaacag agcgatccgg tggacgagtt gcctgcaatg
    25861 atgaaacaga tgcacacaac tcacttcagc agatttatcc gcccgttgaa agatgtttct
    25921 tcatgagact cctctccata ctcatcgcac tggcgctcgt ggccccggcg gcggcggtct
    25981 ttctcacggc ggagaatgcg gaaactgtcg gtaccgggct gaaacaggtc gccacctccg
    26041 gcaacggcac gcgctacgag gcgggatcgt atcatgtcgt ggtcctttcc ggcacctacc
    26101 gggagatggg ccggcagtac ggcgccctga tgaaggatga actccgggcc gagtatgcga
    26161 tgctccggtc gcacttcacg gcatcgggat acaccgaggc ggagatcggt gagctcgccc
    26221 gaaaggcaac aacgctccag gcaaagcgca tgaaggagat ccgggccggt atcgcggaaa
    26281 catcgggcct gaccgtggag gaggtcgata tcctctacga aggggtgccg gtcttcttca
    26341 gtaccatgaa cgagaaagcc ttcttcagta ccgccggtgg gaaggccggc tgctcgtttc
    26401 tggctgtctg gggcaactac accccggacg gttcggtagt cctctcccgg aactgggacc
    26461 acaacgatgc ctttctgatc ttcaagccat actacacact cgcggtctac aacccgaccg
    26521 acgggagcaa tagtgtcgcg acgttcggcc cggtagggtt gctccccgag accctgatga
    26581 acagtgccgg gcttttcatc gccgaggata acggggagag ctccggcagt agccttgaga
    26641 tcgccggcag gcctcaactg gtcggggagt tcttccggtt gatgctcgat tattccacta
    26701 tggacgagct cgatgccggg atcatgtcga cccggacgga ttgcgcgttt atcgtgaacg
    26761 cggccggggc tgaccgggcc tgctcctatg aggagaccgt gtatgatatc aagtgccgcg
    26821 agggtacggg cgtgatcgcg gccgcgaacc atttcgtgga tccgtcgtgg cggcttgcag
    26881 ttcctcctac ggaggagaat acggtcgtgc gatacggcaa tctgctgcgc ctcgccgaag
    26941 agagcaaagg atcggttgac ggggaacgga tggtcgggat ccgcgacgtg ctgtacgacg
    27001 atgggggtgc gacgttttgc cattacacag tcggggatct ctcggcctcc tcgatttacc
    27061 aggtggtctt tatcccggag acccgtacgc tctggatgaa ggtcgtggat cgggactggg
    27121 agaaggtgga actcgggcag ttgttctcgg tgtgagaggg gcggcgagcc gggaatgggg
    27181 gcgggatccc accgcagcgg atcccgcggc cttccgggag ctgccggttt ccggcatgct
    27241 tctccccctc tcaagcgccg tcgctacgta tttcggggga gccccgatcg ggttactctc
    27301 cgaaatacgc gacgatattt cggaagaacc gcatgttcat cgattcggtg gggagcggtt
    27361 ttccctcgcg cttcagccgt tctttccggg tcggccagtc gtagaggttc acgaactcca
    27421 tcgcccgttc ggggtgcggc atcatgccga gcactttgcc atcggctgac gttatcccgg
    27481 cgatatctgc gagcgctccg ttcgggttgt aggggtactc ccctcccgcg ggggagaggt
    27541 cgtcccggca gtaccggaac gcgaccatgt tctccctctc gatccgcgcg agggtctcct
    27601 ccgtgcagaa gaagtttcct tcgccgtggg agaccgggca gtagaggtgt gaaatacctc
    27661 gcgtccagag gttctccgcc gcgggtttca gggtgacgaa ccggcactcg agcacgccct
    27721 ttctgttcgg catcagggcg atctcgcggg aacggtcctc gtcgaacgcg gggacgaggc
    27781 cgagattcgc gaggatctga aacccgttgc agatcccgag gacgaggttg tctcccgcga
    27841 gaaactcctt tacatcgctc cagaggttgt ttctgatgcg gttggcgcag gcgttgccgg
    27901 cgcccatatc gtcgccgtag gagaatccgc cgggaaagac catcaaccgg tagtcggaga
    27961 gggatttttc gcctgcaatg aggtcgttga tgtggacggt gtcggatgcc atgcccgccc
    28021 gcataagggc aactgctgtc tccatctcgg agttgatccc gtagccgctc atgatgaggg
    28081 cttttgccgc ggtcttgacg gtcgtcattc tcagtaccct ccgaagggcg ctttgtatgc
    28141 tgttttcatc gctctgatcg gttgctcgat cagggtctct cctgccgata cccggaaggc
    28201 cgtgccgccg acgcggccga ggaggtgcgc atcgtcgccg aagacttcct cgaacgcttc
    28261 ccggtcaccc ggagcgaccg tgacgacgaa ccggccgggc gactccgaga agaggtagcg
    28321 gtccgggcgc atcccgccgg gtatggtgag atccatgccg acccgtccgg cgatagcgac
    28381 ctttgcaagg gcgacgagca gccccccgtg cgccaccggg aaagcggacg ctataagccc
    28441 ttttgcgatt gcgccggtca tcttctcgta gcgcgccttt gctgcctcca tatcgaggga
    28501 tgggaccacg ccgccctcga tgccgatgtg ggcgaggtac tccgacccgc cgagttcctc
    28561 cccggtctcc cccacgacgt aggcgaggtc gccctcgaac ttcgcatcca tcgataccgc
    28621 ggtggtaacg tcggggtgga tgcctatcga tgtgatgagg agcgtcgggg gtacggatac
    28681 cttcacgggg cgagagtccg cgtcgaaccc ggagaaatcg ttatacatgc tgtcttttcc
    28741 cgatatgtac ggtgtcgcga acgcgatcgc accgtcacgg catccgaacg cagcctgttt
    28801 gagctgccag agcctctccg gctcgtcgga ggagcaccag cagaagttgt cgaggagtgc
    28861 gatggcatcg agcggtatac ctatcgcgat caggccccgg atcgccagat cgatcgcggc
    28921 aatcgccatg cggtaggggt cgagctccga gtacgtcggg aagatgcctt gcgagagccc
    28981 gacacccttc atcgagccgg ggacgacctt tgtcagggtg gcagcggtct ggacccgccc
    29041 gctcccctgt acggggccga gaacgtgccc tccctggacg gtatggtcat accgggtcga
    29101 tatgaactct tttgagcata tgttctttcg tgcgagcatg gcaaggagga ggtcgtcgag
    29161 ccggtcgggg caggggaagt cgggctcggg atatgccgtc tccacgggca atgtcagaag
    29221 gtgcttctcc ggcaggccgt cgtggaggaa gtcaaggtcg atatccatga cggtcgtgcc
    29281 gtcgtactcc acgacgcacc ttccggtatc ggtgaacgtc ccgatgacgg ttgcctcgac
    29341 atctctcttc cccatcaggt ccatgaaggc gtccgccttc tcttccggga cggcgagcgt
    29401 catccgctcc tgcgactcgg atatccagat ctcccacggt gccatgccgg ggtacttcag
    29461 cggcacgcgg tcgaggagga cgtggcatcc gccggactcc tttgccatct cggcgacgga
    29521 gcaggagagc ccccccgctc cgttgtcggt gatgctccgg tagaggtcga ggtcgcgggc
    29581 ctccctgacg atgacgtccg agaacttctt ctgggtgatc ggatcgccta tctggaccgc
    29641 ggtcacgggg ctcgccggat caagggcttc cgaggagaag gttgcgccgt ggataccgtc
    29701 tttcccgacc cgtccgccga ccatgacgat gaggtcgccg ggcagggcct gcttctcgtg
    29761 gagcagtctc ccgttgtgag cacgcggcat cacgccgacc gtgcctgcga agacgagagg
    29821 tttacccacg taccggtcgt ggaagtagca ccacccctgc ggtgtcggta tgcccgaaca
    29881 gttgccgccg atgccgacgc cgctgatgac gccctcaaag atccttcgcg gggagaggat
    29941 ggggttttcc cggttcttcc cgcggaagag ggccggttcg gtgtcggggt cgccggtgca
    30001 gtagccgtat atgttgatgc agggtttggc gccgaggcca aacccgatcg tgtcgcggtt
    30061 caccccgacg atcccggtga gggcgccgcc gaaggggtcg agggccgagg gggagttgtg
    30121 cgtctcgacc ttgcaggtca cgaggtgctc gtcgtcgaag atgatggcgc cggagttgtc
    30181 ggtgaagacc gagacgcaga tgtccgcgtc ccccctcttc ttgcggatgg cgttggtggc
    30241 cgcctggatg caggtcttgt agagcccccg agggacgtcg tcgtccatcg ccgacgcaaa
    30301 gatggtgtgc ttgcagtgtt cgctccaggt ctgggcgagg gactcgatct cgacgtccgt
    30361 cggcatccgc ccgagccccc tgaagtagtc ccgtatcgcg tggagctggg cgaggtcgag
    30421 cgcgagcggt ccccgccgct ctcctgtccg gggatcgagg ataccctctt ttccgatgcg
    30481 cgcgaggtcc gcgtcggaaa gatcgaggcc cacggccccg gccgcctgcg ggtcgtcgag
    30541 atggacgaag ggcgtgaccg catccatccc cttcttgccg tactcctcgc ggcttttgat
    30601 gtggatgcgg ttgatgagcg ggtttgcgag ggcgccggcg agtttttcca gggattcggg
    30661 agtcaggtcg ccggcggcga ggtagagctg cgatgagaag accgcttccc ccttgccgaa
    30721 ctggaatccg aagtagtcct cgatggtctg tcttgccgtc gctccgacgt tatcggtgac
    30781 tccgggaagg aacccgacct cgattgcatg atcaaagtca gcctcggtcg gccggtccac
    30841 ggagtagcgc tggacgacgg ggttgctgag ctgcgcgccg atctgcgaga gttcttcggg
    30901 ggagaagtct cttttactgg tcgctatagt atagacgtcg acgaggtgca gctcgcgcag
    30961 ggggatgccg agcgagcgga acctctcggt acgcatctgt aaccgtggat cgcaggagta
    31021 gtgtacctct atccggtgga cgtgagaggc catgtatata tatgggtgcg atcgggtgaa
    31081 aagagctgcg atggagatga tgcacggccc tgtaccgggg tcaaaagagg tccgggcagg
    31141 catcgtggcg cccttcttcg gcctggttct gaacgggtta catcccgtcg gcgatgccgc
    31201 cctcgtaacc tgctctcgcg aaatctgtaa aggggttcga tgacatactc acccggagga
    31261 agccccgtat acaaaaagca ttattgcgag agtatcaacc gtggaaggat gcgtaatgtt
    31321 cgaccgttgt cctggtgctg cacatatccg aactccgacg ctgaagataa aaaaatgccc
    31381 cgagtgcggt gcggaggtcg aggtcttctc caatgacatc tcggtgagat gcagcgaatg
    31441 cggatttacc gtttacaacg atgccttatc ctgcgtccag tggtgtaagt acgcacggga
    31501 atgcgtcggt gaggagacct accagaaact tgtaggcaaa aagccggatc gctgaaaggg
    31561 gagagggttg tgccggcatg caaacatccc tgagtctgat cctttgatcg aatccatcaa
    31621 atcggagggt gtccatacgt gcggcgagcg gacattttcc ctatcatccg gctcaagacc
    31681 tacaaagcgt gaaagtgatc ctccccaaaa ctatatcatt cccgataccg acatcatctg
    31741 tatgccgacg aagacggtag tcgaggggaa gaaggcgacc ccgaagaccc cgcctaaagg
    31801 aactcttact caggctgaaa tccaggccag tatcgatagg ctggatgcag acatggaaag
    31861 atgggggaag ggtcggagat acactctggc cgaggtagaa aagaggctgg agttataaat
    31921 cgatgtgtgg gtacttcttg tgcgccgcct cgaaacccat gacctcatga atttccacag
    31981 atgttttttg ctctgttttc accaccttat aaaacaccgt gtacttcctc tgaatatgta
    32041 accggtgggt gttctccttc cctttcaact gcttcttatt gcattgtttg atcgaagtga
    32101 acaggtgttc gcccaggcag aattttattt tatcaatgat ttttttgata tactcgttat
    32161 ctccacgctt gattgcctga aggtgcctct ctgccgtctc tgtaattacg atttcgtatt
    32221 tcgtcatgcg cctctctcag gcctcgacgc cccgtgcatg cagcgcggcg atcacaccct
    32281 gttctgcagc ctctgactta ggtatgcgct cctccttcgc gaggaggcag acgttctcac
    32341 ggatacgagc cgaggtttgc agaaagacct ttcccttcgc gttgatgtgc ttcatagtca
    32401 gtaggtggca attatagtct cttaaagagg tggtgacaga aacagaggtg tcggcaactc
    32461 gaatccctca acatccggcc cccgcaacct gtttttagtc acctctctta tcaggccccg
    32521 cgcagagagc tatgcatgcg caatctgtca tgacggttat gtaactgtca tgacagtgga
    32581 aaggagaaga gaattatgga gcgtaaggag aaaaaggagc aatacaggcg taaaaaggag
    32641 ggatatacgt tcacactgtc cgatcaggca tatgtatttc tcaaaaacaa aattacaact
    32701 gtgcctcgat ccatataaag tctcataaga tatgtcaaaa cgaggattct gtcacaaatg
    32761 gtgccgttaa tccaatcgag gtaagcccca gcggagcaca gcgcccgcac tctggagata
    32821 cgaacaaatc taaaagagcc ccagccgtga tttgaacacg ggacctgctg attacaagtc
    32881 agccgctctg ccagctaagc cactggggca taccttatat cgtgagttct ctgcggtaaa
    32941 aaaggtggcg ctgatcctcc tcatgccttc ccggcatcgc ccggccgatc gccatgctgc
    33001 cacatggtct ccccctcata ccgtatccgc cggacccggt gatacggaat acacgctgcg
    33061 tgcgttccgg cgtccacctc caggtactgt gcgtcgagcg cgacgacccg gtcgccctgt
    33121 acccttgagc ggtcgccggg cgcccctctg tcgacgtact cgacctcgac ttgcgagaag
    33181 tcataccccg gatcgtgata gaaccggagc agcagccggc ggctcgttct catatgaggt
    33241 tagagcatct ccatagagta taaaaccctc ccgggaccaa cggtgattct acaggtactc
    33301 ctcgaccgtg ggagcacgat gcggacatgt accgatcgga acagaggtcg agcattgcag
    33361 gtgtgtgagg ggatgtgaat ctcgtaacta cgatagccgt catcgtggcc gttcttctcg
    33421 ctctcctcct cgcgggccgg gcggggctgc aggtacaacc cgctcccttt ccaccgccgg
    33481 cagggccggc ggccgcaccg gagacggttc cgttgccgga aggcctgcct ccgcccgtgg
    33541 agcatttcta ccgcaccctc tacggcaacg atctgccgtt gattgagtcg gcggtgatca
    33601 gcgggcgcgg caccatgcgc atcggcggga tcacattccc ggtgcggttc cggtttaccc
    33661 acgaaaccgg gcgaaactat caccactaca tcgaaaacac gttcttcggg ctgccggtga
    33721 tgaaggtcga cgagtactat gtcaacggca ccgcccgcct ggaactgccg ttcggcgtcg
    33781 cccgcggttc gaagatcgac cagggcgcca acctcgccct gtgggccgag gctatctgga
    33841 tgccggccgt ttgggccacc gatccgcgaa cgcactttga gcccgtgggc gagcatacgg
    33901 cagagctggt ggtcccgttc ggcaatacgg aggagcggat caccttgcac ttcgacccaa
    33961 agaccgctct gctgcaggag atgcagtcga tgcgctacag gggcgaagag agcgaggaaa
    34021 agaccctgtg gctgaacgac gtcagggaat ggagccggat ggacgggtgg ctgatccccg
    34081 tgaccatcgc agttgcctgg ggcgatgagg cctctccctg ggcggtgttc acggccgaag
    34141 aggtcgttta caacggagag gagccccttc gaaccggcgg agtgtaaccg caaggcaaat
    34201 accagagcat aacggtgcag gatgtcgccg gagggcgggt ctgcaacgtt cataaccgag
    34261 gaacacagaa gtaccagcag gatgtcgcat ctcaacgtcg atctcggagg ccggcccggc
    34321 ctcgactgcc ggggattctg ttcgtactgc tacttcaaac acgtccaggg gacgacgtcg
    34381 tttggctgca aatactgcct cccctttcag aaaggctgcg actactgcac ccggggcgtg
    34441 cgcgagggtt ataccgggtt caagagcctc cggaccgttg ccgacgagac gctcgcaaac
    34501 ctccaggtga tgagcgacga cgtagaccgg atcaccatca gcggcggcgg ggacccgagt
    34561 tgctacccgg agtttcgcga cctcatcgaa ctgctcggca gcatggaagt gccgctccat
    34621 atcggctaca cgagcggcaa ggggttcgat gaccccggca tagccgatca cctcatcgag
    34681 aacggccttg ccgaggtctc ctacacgatc ttcgccgccg accctgacct gcggcggcgg
    34741 tggatgcacg acccgacccc ggaggcctcg ctcgcggtcc tcgagcggct ctgcggtgca
    34801 atcgacgcct acgccgccgc agtcgtcatc ccgggcgcga acgacggcga gatcctcgaa
    34861 gcgacctgcg cgtggcttga ggatcggggc gcaaaagggt tgatcctgat gcggtttgca
    34921 aaccgaatcg accaggggct cattctcggc aacgcgccca tcatcgaggg gcagcaggtc
    34981 cataccgtcg atgagttcca cgacctcgtc acccgcctca atgaacagtt ctcgatgaag
    35041 atcagcggga caccgctcgg ggacccctcg atcggatccc cgtttgcgat cctccacgaa
    35101 cccgacctct tgaagaaact ccccagggtc cggggacacg cgacggtgat caccgggagc
    35161 atcgccgccc ccttcatcca gcgggtcctc tcgatccgtg gctaccgttc acgtgtcgtc
    35221 agggtcagaa aggagatcgc ctgcctcatc acggccgagg acctcgccgg cgtgaactta
    35281 tcaaaactcg aagacgtcgt gatcatcccc ggccgggcgt tcgtccacga cgtcgaggcg
    35341 agaaggatcc tctccgccga tggcgtcgac cgcgaggtgg tgcgcggccc cgacacgctg
    35401 acggccgatg cagagacgag catgggcatg acccggatag aagtgctcga gaaagagatg
    35461 gaggggtttg cagccctgat caacacgatc aaccagtacg gtcgatgaga gaccgttccg
    35521 gccggggccg gatagcacgg gatcgaggcg atccttgccg gagcgctcgt ccggccgccg
    35581 gcgtgaggcg gccttgaacg agcgcggcac cccctcaccc cttcgccggc atggtccggg
    35641 tatggtgtaa ccccctgtcc ccgggcattc acccactgtg cgtatggggg gaatcagtgg
    35701 gtcctcttca ccatcgggac gaaccgctcg atgatccact cgttcctgac ctccgggtgg
    35761 aaggaaaggc cgtaaagagg gagtgagcgg tgccggatcg cctggatgca ccggtccgat
    35821 acgacaaggg gtatgaatcc ggtgggggtc tccacggcgt gctcatgcag ttcgtacgca
    35881 gggaagcctg tcatcccggc aaagagggga tccggcgcga ccacccggat atccaccata
    35941 ccgatctcgc agcacggcac ggtgccgccg ccaaaggccg ccgcgagggc cagcatgccc
    36001 gaggagatgc cgagcaccgg gcgtgcgaac gtagagagcc accggaatat atccgggtga
    36061 tccagaaatc ccgtatcctt cagcgccgtc ccgcagagga ccgccccatc cgcaccttcg
    36121 atatccggtg cgctgacggt tgtatagtga cggacgatgg gctctgcccc ggcctcccgg
    36181 aggtagcgct cgaccggccc gacgaactcg gcgtgggaga gcgagttgcc gcgatagcag
    36241 agatcgataa ccaggatcac ttctccacca gtcctgcccg ttcgatccgg aggatgccgt
    36301 tgtagttctg gtagaccgcc gatgcctcct cgatcgctat cctccgggag aagaacgggg
    36361 cgtcgatgac gaacgtctca aactccccgc cttcgccggt gagagtggtc cggtacttcc
    36421 gggcgacctt tcggaggtcg tcgagggtgc ggcggtcgat ctccctcccg agccaggatg
    36481 catcgaaggg cgaggagaag acgcccacga cgatcacccg gaatccggca tcgatgagct
    36541 cctccatgta ggcctcctgg tcgatatgcc agagcgggtt gaagcaccag aggtccagtt
    36601 cgcggcagat tcgctggacc cgtgcggcct ggtagaccga gaggatcgct cccgtaacga
    36661 ctccctcgat atcgaaccgt tcccgggcga gggagagagc tcgcttcagg tccaggagtt
    36721 ctgtctcctg ctcccctgcc gtctccacct ccacgagggg gagccccgcc gccgcggcct
    36781 gcagcccggc aaggtggatg ttcggggtat ggaacatata actctcgggg tttttggaga
    36841 cgaccgtgat caggcaggcc acctcctcat gctgcatcgc tttccagcag gcatagagag
    36901 agtctttccc gcccgagaag agcacgccga gtctcatggc tgttcgcgta cccgttctct
    36961 ttcgggcacg ataacccctg cggcggcggc gccggaagca cccgggcggt ggaagtccgg
    37021 gttctgcatg ccgtcccgac caatcgcgcg gttaccggcc gccgccgccg gggggatcag
    37081 agttatatgc tgttgaacct tcaccggatg tgagcaccgg gggtgcatcg ggagatcttg
    37141 ctatggtggg agtcgggcag catcaagaag agcaggtcga agtggttgtt gattgcatcc
    37201 tggtaaacta tcggggggat cccgagtcgc ggggccgtgt cgtccatgct ctcctcgagt
    37261 gggccgaaga gcaccccgat gaatggagcg aactacaggc gcgctgtcag cagcgtcacg
    37321 aagcgctggc gtaagccccg gaaacactat ctcttcttcc ttcctgagct ggatgcgggg
    37381 gtatctgcgc ccggcgtcgc gacgactatg ttccggcagc cgggatggcg ctggtataaa
    37441 atgtttgaaa aatgcgaggg gtgagattcg aactcacgaa ctcctacgag accaggccct
    37501 caacctggcg cctttgacct ggcttggcaa ccctcgcccg ggggagagaa catagtgaac
    37561 gcgggatgga gaatgcactg attgcgcttt cgcgccccgc ttaccttact taatctgttg
    37621 tcatacatga aatacctttg tgcggagagg tggtagggcc ccggcaatgc cccggagggc
    37681 gctgatcaaa gaggccctgc actatgtcgt gcagggtata cggtcacacc ccggtcggga
    37741 aaggctgttt tctctatcta gcctctctct atgcggaata aggctcccgc tcttcataca
    37801 agcgtaaccg tcgggctgcc gtgtctcacc cgcgaaggat gctcccgccc ttcagtggtg
    37861 gcgtggcctt gattaaccgc cgggttcaat acgctaccat gaaggccccg ttgctggtcg
    37921 catgcctctg ctgtgcgttg attgtcacgc tctcctccag cggctgtacg accaatacta
    37981 aagatcagga atactacgac aattgggttg cagcagagcc ggacaacgcc gaggcgtggt
    38041 gcatccgggg gatgtactac aacgataatt acgggcagta tgccgaggcg atggagagtt
    38101 gcaacagagc gcttgaactt gatccgggat acgggctggc ctggtacctg aaaggggtta
    38161 ttctgacgaa catgaatatg accggtgaag cggcgccatg ttttgagaat gcaacgaggt
    38221 atgatcccgg cctggcaaaa agcgtacagg ccgttgccgg caacacgcgg tgatatggac
    38281 cccaatatcc cttaaatatc gattgttacg agatccggga tgaatgcttg ggggaggtgc
    38341 cggcagctgc gcgaggccgg ccctccgcct atccaggaca ggggatgatg ggtataccgg
    38401 ggctcaagcc ggcagaggca gccggcgagg gcatgcggga gcagccatcg gggatccctc
    38461 atccatgatg gctgttcccg cctgcccgga gcggttttgg cccgcggtac acgggaatat
    38521 accgaagtga gagcgtccga ggtctggccg cgcgccccgc acgtatatac gaaacggccg
    38581 ccaaacctga gaccacaggt gaataccaga atgtacgagg attcatacac caaccggtta
    38641 cgcaagaccg ccctcgatat gggtgcggat ctcttcgggg ttgccgatgt gcagaacttc
    38701 gatgacccgg catacgcggg caacgatcct cggggtatca tggaggatgc acgcgcggtg
    38761 atcgtcatcg gggtggcggt cccgcggggc tcgatcgaga gcctgccgaa gggcagggcc
    38821 gagtacacca acaccctgat ggcgggcacg gcgacgatcc ggatcattgc tttccggctg
    38881 gcgcgcctga tcgagaagga gggctacaag gccacgatcg ttcctacgga gggcagcgag
    38941 ttcgggtact ggtatgcaga ccgcgagacg ctcatggcgg ggatgtccat caagtacgca
    39001 gcatactgcg cgggtctcgg gaactacggg gcgaaccatc tcctgatcac ggatgagttc
    39061 ggcccccggg ttcgcatgac cgccatcctc acggatgccc ccctcaaggg gccggaaggc
    39121 ccccggcggc ctctcctgaa cgaggactgt aaggactgca tgaagtgcgt cgaggcctgt
    39181 ccggctgcgg cgttcaccgc cgatggagag attcaccggc agcggtgcgc cgagtatatg
    39241 tttcaggcgc tcggtgggct ccggtgcggc ctctgcatca aggtgtgccc gaagtgagag
    39301 ggtaactccg ggcagggccg gtagcgaacc cgggcgccgg tcccggggaa tctttctcat
    39361 ccgcaggcgt tgccggatca ggaatattct gtttccggag cggaaacaat ctgttcgcgg
    39421 ggcagaagcg g
//
LOCUS       DAOSEE010000004        34070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_645_length_34070_cov_247.265442, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000004 DAOSEE010000000
VERSION     DAOSEE010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 34070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34070
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_645_length_34070_cov_247.265442"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PLI76_00730"
     CDS             <1..366
                     /locus_tag="PLI76_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60582.1"
                     /translation="ALDEQDRLAPLEGFWIYSAGPTAVPLNFSTNPLLPPAERTLVAG
                     WNAIGTTGNTPATARDTLYSVKGRWTTLIGFDARNQAFETGIVNGGSGANTDTRSVYP
                     GRGYWLSMTGPGTLYAIGA"
     gene            372..>1393
                     /locus_tag="PLI76_00735"
     CDS             372..>1393
                     /locus_tag="PLI76_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60583.1"
                     /translation="MRHTLWILVLLFALIGAASAIPALPAEFSGTVTIDENPAPAGTA
                     ITARIGDRDCGSLTLKTAGTFGGDSTFDKRLLVNGEDTDLGKAITFFVDGISAGTATY
                     TPGTSTDLALAVTKGDTPDSESSDGTPPGSSGSGSSSGGSFTPPAAPATEYTGSGTLD
                     TDARGAVQHAAVIAAAGGDARLTIAEGVLAQDRFERPLDTVSVETASPGDATRAVRCG
                     PDGATFDPAIEVSFALTPEEWEQAGQFVVRWYNSTADVWEPLATTIHASTRTVTATIS
                     HFTLVAVFAVPADTPEDTVTATPAGSETPTSTPAAPATTVQQAGPSLPGLAGLAALAG
                     AALLIR"
     assembly_gap    1394..1493
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1613..1735
                     /locus_tag="PLI76_00740"
     CDS             1613..1735
                     /locus_tag="PLI76_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60584.1"
                     /translation="MEMRPGATPVVTTDAGAKILSSSGADLFSRSVEDGRERTR"
     gene            1713..2063
                     /locus_tag="PLI76_00745"
     CDS             1713..2063
                     /locus_tag="PLI76_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOI60585.1"
                     /translation="MDERGPGEGRGHRGRGRPRVRRMIGDGGAFRCFGPLCGRPDEVV
                     LLLPEEIEALRLVDLAGFEQEEAAIALGISRKTLWRDLHEARKKVADALVHGKTIQIV
                     GCGRRRGERCPGDE"
     gene            complement(2159..2725)
                     /locus_tag="PLI76_00750"
     CDS             complement(2159..2725)
                     /locus_tag="PLI76_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleic acid-binding protein"
                     /protein_id="HOI60586.1"
                     /translation="MTPQTQFEREPARRVFASELRETRYQFKDGDDEKSPTYVILPTG
                     IRSNRVFIVGTLTEKLRQGDQNIFYRGRIVDPTGTFFVMAGSYQPEAMQQLARIEPPA
                     FVAVVGKPSLYTTPDGTCLVSVRAESISIVDRETRDLWVLDTARETLDRLDAFGTTDD
                     SRKAQEEYGTQPAIYRKIVYDALSQVQL"
     gene            complement(2722..3948)
                     /locus_tag="PLI76_00755"
     CDS             complement(2722..3948)
                     /locus_tag="PLI76_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HOI60587.1"
                     /translation="MNLSEVTERISRKLEAKGAQPDRQKIESRLRRLVEEFGVNAAEA
                     ERTVTGDLAREYKVTNIGGTSTELRPISDIVPGEWVTIEGKIVALTPALSPSIAQSGI
                     VADSSGAMRFVTWTRANAPAMEYGHWYRIESAVVDEYKGAPNLKIHSGTTISRMEKDA
                     PLLPSITPIAELKPGVGSVRAKVVQDWEVSHERMLQTGLLGDETGTIKFVTWNEEGKD
                     KLDLDAVYNIFYATVDEYNGRLSLTLNTAMYIADEGDIEVGRSETEIQGALVHIAPGS
                     GLIKRCPVEGCNRTLSRQNYCPVHEIQPNFRYDLRLKAVLDDGIRARNVLVQRELVEK
                     LAGITLDEAVGIAESNPIGMDEIFYRIRNAVLGRYYTCSGSEFGGRLLASSCTPVAFK
                     PEELAALLNRAGGESA"
     gene            4223..4417
                     /locus_tag="PLI76_00760"
     CDS             4223..4417
                     /locus_tag="PLI76_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HOI60588.1"
                     /translation="MALTADSTIAELLREKPESVQVLFRFGMGCLGCAIANNETIREA
                     AQVHGIPLEEMLSALGVAEA"
     gene            complement(4426..5313)
                     /locus_tag="PLI76_00765"
     CDS             complement(4426..5313)
                     /locus_tag="PLI76_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide amidohydrolase"
                     /protein_id="HOI60589.1"
                     /translation="MRHFVRDDTLFLRGRFRAASTGINGGIADVTTVLNHTVSRDFCD
                     EPQRYLELLAARHGLFHEYFGLLTAVEMRHLCVLQYDFITVFITAGVTNPTPPVDNAP
                     HTINIVVHSREGVCDAALLETIVTATGAKARALHDLGYDFPGTTTDAVVVACERDVPG
                     AHTYAGTLTEAGRRVHAAILYGVPEALARHQGKVRRSGPSFFIYSRYGGDHWVEWVKE
                     GCPYYPCHFPGQRCDYCYCPCYPCADEELGEWVESSNGGRVWGCAGCTLLHVPAIADY
                     VKRNPEATLAELKRLRERL"
     gene            complement(5310..5672)
                     /gene="nrdD"
                     /locus_tag="PLI76_00770"
     CDS             complement(5310..5672)
                     /gene="nrdD"
                     /locus_tag="PLI76_00770"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HOI60590.1"
                     /translation="MNWSPEQQKLVEKYRSLNDIPAGERRYKCHTCHFVVDETPCPHC
                     GETALEIMCPLDHCDCHHSIVESIEYCPLCGHAVCPECGSHDVVQISRVTGYLQDVAG
                     WNAGKQQELKDRVRYSAV"
     gene            complement(5788..6423)
                     /gene="pyrF"
                     /locus_tag="PLI76_00775"
     CDS             complement(5788..6423)
                     /gene="pyrF"
                     /locus_tag="PLI76_00775"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOI60591.1"
                     /translation="MTELILSLDVTDRRRAVAVAESCAPFIDAIKVGYPLVLAAGLSI
                     VGDLSGVGLPLIADFKVADIPNTNRLICEAVFSAGFDAVIAHGFVGGDAARACVEVAH
                     NHGGAAYIVAEMSHPGATEYFHGGVAEHLAALAVACRADGIIAPATRPDRIGRLREIV
                     GERTIYSPGVGAQGGDLDTVARLVDGVIVGRSIYEAADPGAEAERFSRIRR"
     gene            complement(6420..7355)
                     /locus_tag="PLI76_00780"
     CDS             complement(6420..7355)
                     /locus_tag="PLI76_00780"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HOI60592.1"
                     /translation="MTFKYGNAVQQARVRPGMTVGELVDELGRAGAYNGGSLWQAVNI
                     YERMLRDEEALKFFGLSGAMVPGGMGGIVADLIRRRHIDILVSTGANLTHDIIEAIGC
                     HHYHGVADVSDAELCEEGINRIYDVFLPNEAFIRFEEFMQDVYSSLPEGSTVSIAELL
                     NRIGKRLDTGILAEAAKAGVPVYCPAIQDSMIGLQYWLFSQTHKVTVSAFGDMPALLD
                     RCFEAERAGAILVGGGVPKNYILQSKLMTESGFDYAVQLTGDRPDLGGLSGATLDEAR
                     SWGKLTGEATAVTVYGDATISLPLLVAATLERLEG"
     gene            complement(7878..9131)
                     /locus_tag="PLI76_00785"
     CDS             complement(7878..9131)
                     /locus_tag="PLI76_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Ile)(2)-agmatinylcytidine synthase"
                     /protein_id="HOI60593.1"
                     /translation="MWIGIDDTDSPAGMCTTYIGAVLVRRLKERGIPITGARLVRLNP
                     NVIHKTRGNAAVCIEAEGDAEAVFALACACVEELAEFDAPETNPGVVVAAVRPPPDFY
                     YAVLRDFCTVDEAVDVLESIGARYRGYKNRRGLIGATAAVASVFFDRTYELLAYRKRE
                     AWGTPRQVDRASLFLADETTYPRTWDTVDRKNDAVVCVPHTPDPVLFGIRGESPAAVR
                     EARSYVRSEEPACEQVYTTNQGTDAHLLPGAIGELREGRSYLVRGAVAGLPATGPGGH
                     VSFLLEDGGVEVRCMAYEPTRGFRDVVRALVPGDRVAVAGSYKGGSLNLEKLGVSHLA
                     ALVRIRPPVCPACARRMTSAGTAKGYKCRVCGGRSREPDVERWERRIRPGWYEVPPVA
                     RRHLSRPLVRGVPAWEEDLLQSGRP"
     gene            9178..10092
                     /locus_tag="PLI76_00790"
     CDS             9178..10092
                     /locus_tag="PLI76_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOI60594.1"
                     /translation="MSQDRLPQMVISIMLLADFDISERCNIRPRSFDLIAKKGDTLVI
                     IKVASQIDSVTADIAWDLNLIARYLQATPLIVGERARDAELERGVVYIRYGLFALSSE
                     TLYDYFAEGISPMVYASPGGLYVKIKGDLLREVRERSRMSLGDLASHLGVSRRTISKY
                     ESGMGTTLDVAIRLEELFSAPLVETIELVGYRTPEPENPPKSTVGDVFIDLERMGMEI
                     HAMRQAPFQALALFDRHTILTAYGTTQKVVKRASLIGNISQITKTFAMCVVTDYNKQK
                     KIGKTLLIGEEHLHTLEDGSELIDMINE"
     gene            10150..11814
                     /gene="thsA"
                     /locus_tag="PLI76_00795"
     CDS             10150..11814
                     /gene="thsA"
                     /locus_tag="PLI76_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit alpha"
                     /protein_id="HOI60595.1"
                     /translation="MSSLGGQPILILKEGSQRTRGRDAQSGNIAAAKAVANAVRTTLG
                     PKGMDKMLVDTIGDVVITNDGVTILKEMDIEHPAAKMMVEIAKTQDDEVGDGTTTAVV
                     IAGELLKRAEDLLDQDVHPTVIAHGYRMAADRAQVILDEIAVDVKPDDMAMLKKLADT
                     AMTGKGAEAAKEKITDLVVRAITMVADADGTVDTEFVKVEKKVGGSIEESEIVEGMII
                     DKERVHPAMPRVVKNAKILLLNAAVEFKKTEVDAEISITSPDQLQMFLDEEERMIKGI
                     VDKIIASGANVLFCQKGIDDIAQHYLAKAGIFSVRRVKKSDMEKLARATGGAIVSSID
                     AIAPEELGKAGTVEEKKVSGEEMTFVTGCENPKAVSIIIRGGTEHVVAELDRAIEDAL
                     RVVSVAVEDKKLVAGGGAPEIELSLRLREYAATVGGRAQLAIEAFANALEIIPRTLAE
                     NAGLDPIDMLVALRASHEKGGANGKYMGLDVFNAVSGDMLKAGVVEPLRVKTQAVSSA
                     AEAAVMILRIDDVIAASKSAGPSPEEMAAMGGGMGGGMGGMGMPPM"
     gene            12072..13964
                     /locus_tag="PLI76_00800"
     CDS             12072..13964
                     /locus_tag="PLI76_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOI60596.1"
                     /translation="MTLRGSVRQALPFTIYLLLAVLLAMAPVIYLISFVGYADVRQAL
                     VADTGELRGQTEYGIVLAVNLVDAGLKLFDDTLNREMQEGFGPFAVEYERAGRDPGVM
                     DLARVKEELGGRMDLYVINENGIIEYTTYPPELGYDFKEIPAFYDRITDIRLNGAFSA
                     DRIVPELATGMLRKYAYQPSPDRRYLFELGQVVSESGGQRMALSYADAIREVADENPG
                     VSEIRVFDCLGERIVGAAHPDDDRRLGMVRKAYRERTMLETENVDTGELIRYLFVDRT
                     DSVHASDMSVVVELTYNTKFVEDAIAGMFARHTQILLIGLSLIACLSALAIRSLTRPV
                     RILVEDVDAVARGDLDRPIRVSGSEEFVHLGGSVSTMIASLKEILQRLQESEEKIIRH
                     NRTLEDEVGARTAALEESNRTASLLLDIMGHDINNANNIANLYSDILLTDLEGEPEAE
                     LLRKAKAGLAKSIGIVRNVNTIQEIQGKPGAIGKIDLDRIVRSEIRSLPDLRITYNGT
                     TAAVIADDLLSEVFSNLLGNAAKFGGPDIAIAIRIEEHEDDLIISIEDTGPGVPDAVK
                     PRVFNRLARGNGEAAGTGLGLYICRMLIARYGGRIWVDDRVAGHPESGTAVRFTLRKA
                     DEGGSP"
     gene            13961..15844
                     /locus_tag="PLI76_00805"
     CDS             13961..15844
                     /locus_tag="PLI76_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOI60597.1"
                     /translation="MTAVDGGRWHRRLGTHLLLAMILITLPVIGLISALDCNEVVGAL
                     MAGDELLREQTEKSVVRSVRLADAGLKLFDGTLDSRMAEGFDPVLAGYERAGRDPGAM
                     DLARIREELGEEMDIYVINESGIVEYTTYPPDIGLDFRKLPTFYDRITDIRLSDVFVA
                     DRVVTEPAGGILRKYAYQPSPDHRYLFELGLVCNATEAGRYAPDYRALREDLMRLNPA
                     LKEVRIYDSYGRLVNVTGSESPVVSVAVDPAAIAIFEGREGRTLANRTVGTITRYIFI
                     DLSDPASPSDKSLVVELTYTTTLLDARIADVQLGHITIVLFASLAACCIAIPVSRRIT
                     RPVQEIVDDADLIAGGDLDHRIRVSTGTEFARLSESIGIMVDSLKGNIRRLQESEEAL
                     RQYDARLEDMVRERTVELEESGRTANLFLDIMVHDINNANAIAIGYTQFLVDALEGER
                     KEMAQKMLSGLEQSSGIVGRVATLRKARESVTTLTPMDLDRVIRTEVAGHPAARIRYE
                     GRPVDVLADDLLPEVFANLIGNAAKFGGPDVAIAIRVEEREGEVTVTVEDTGPGIPDA
                     MKESIFGRFRKGEESLAGAGLGLYICRTLIARYRGKIWAEDRVTGHPESGTAIRFTLR
                     TAG"
     gene            complement(15847..16041)
                     /locus_tag="PLI76_00810"
     CDS             complement(15847..16041)
                     /locus_tag="PLI76_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YwbE family protein"
                     /protein_id="HOI60598.1"
                     /translation="MNGQNRAAVHPGMTVDVVLKRDQKTGKRTRGVVAEILTNSSYHP
                     HGIKVRLRDGQVGRVQEIIG"
     gene            complement(16058..17584)
                     /gene="lysS"
                     /locus_tag="PLI76_00815"
     CDS             complement(16058..17584)
                     /gene="lysS"
                     /locus_tag="PLI76_00815"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOI60599.1"
                     /translation="MSDMDHLCFDPTKIDKIRDLLERGVTVYPYTFERRDTVRNIKER
                     FSTIGHEKSAEEVSTAGRIYIVREHGKTLFADMGDSDGRIQLYLRKNDLGEEQFDLFR
                     QYVDAGDIVGVVGHAFRTKMGELTIWVDRFELLAKSICPLPEKFHGLKNVETRYRQRY
                     IDLIMNEESRETFRTRSRIISLLRQFLFERDYLEFETPTLQPVYGGANARPFTTHHNA
                     LDQKLYLRIAPELYLKRLVVGGFEKVFEIAKNFRNEDIDTNHNPEFSMVEIYEAYRDY
                     NDMMDLTEEILSFLAEKVCKSAIVPFAGHDLDLSLPWRRLTMEEAVREYAGIDVRAMS
                     LEELRAFGLAHCVEGCESAATPGEYLVLFFEHFGEKNLIQPTFVYDFPVENSPLAKKH
                     RTKEGMTERFELFIAGMEMANGFSELNDPLDQKARLELQDARRRKGDLEAQMIDYDFI
                     NALGYGMPPTGGVGIGIDRLVMLLTGKDSIKEVLLFPQMKTAVPGQNGDKTEESDGEE
                     "
     gene            17935..18552
                     /gene="udg"
                     /locus_tag="PLI76_00820"
     CDS             17935..18552
                     /gene="udg"
                     /locus_tag="PLI76_00820"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type-4 uracil-DNA glycosylase"
                     /protein_id="HOI60600.1"
                     /translation="MVRETGREVAIERLAAVISECRKCPLWENTHHAVPGDGDIRAGL
                     MLIGEAPGKQEDLTGRPFVGRAGKLLDGLLSGIGLTRDDVFIANIVKHRPPGNRDPRD
                     EEIRACTPYLEEQIRIIEPGVIVTLGRHSSRYILSFLAIEFDRITDIRGTVYPGSLFG
                     RPVRLIPTLHPAAALYNPAYREALAEDFAIARRELARTGGTAELD"
     gene            18559..18990
                     /locus_tag="PLI76_00825"
     CDS             18559..18990
                     /locus_tag="PLI76_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOI60601.1"
                     /translation="MTREKSYGAVVFRRDADLQYLILQYGAGHWDLVKGHGKRGESEK
                     ETVLRELAEETGIAQATFIPGFREEIHYFFQRRGRTVYKEVVYYLIETPEERVLLSDE
                     HIAYQWLPYGEALQAITFGNSKRVVSEAHEHLTMLQSRESG"
     gene            complement(19008..19343)
                     /locus_tag="PLI76_00830"
     CDS             complement(19008..19343)
                     /locus_tag="PLI76_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HOI60602.1"
                     /translation="MALETFIGVVGLVIGAFGAFFIVYGAMIAIVELLARETRIRERS
                     YHDIRGVFTTRILIGLEFFVAADVLKTILKPTFEDLIALGSIVAIRTVVSYFLGREAS
                     EIPEDRMER"
     gene            complement(19504..20274)
                     /locus_tag="PLI76_00835"
     CDS             complement(19504..20274)
                     /locus_tag="PLI76_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI60603.1"
                     /translation="MILEVDGVAFTYRSAPVIREVTFGVRPHQILAILGPNGVGKTTL
                     LKCMNAILKPKAGSIFVDEIDLLRADRMEIARKVGYVPQRCEAGRLTAFDAILLGRRP
                     HIRWDVTEADLRIVEAAIRMLDLEALSLRYIDEMSGGELQKVSIARALVQEPRVLLLD
                     EPTSSLDLKNQLEILGIVRSVVTDHDVAAVMTMHDLNMALRYADRFIFLKEGVIHAAG
                     GHEVVTPETIEAVYGVPVTVERYNGFRVVVPLASGSTC"
     gene            complement(20271..21383)
                     /locus_tag="PLI76_00840"
     CDS             complement(20271..21383)
                     /locus_tag="PLI76_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOI60604.1"
                     /translation="MHFADGTVPREYLGYVRRKHLWILGGIALLFILLVVSISVGAAG
                     VPIYDVFLSIVNGTADRIFVFLNPGATMSVSNVDRIVWNIRLPQALAAIVAGVGLSVA
                     GVAMQSILRNPLGSPFTLGISNAGAFGAAVSVILLGTGQMHSSVANAVTLNNPYLTTV
                     VAFIFCLLATAVILIISRVRGASPEVMVLAGVALSSLFTAGTMFLQFFASDTQLAAVV
                     FWTFGDVSRAGWQELGIMTFVVAAASIFFLANRWNYNAIDAGDETAKGLGVSVEAVRN
                     IGMVVAALVSAVIVSFLGVIGFVGLVCPHMVRRLIGDDQRYLIPGSCVAGGILLLGSD
                     TVARIIVAPYILPVAVLTAFLGAPVFIYLLLRGYRR"
     gene            complement(21410..22564)
                     /locus_tag="PLI76_00845"
     CDS             complement(21410..22564)
                     /locus_tag="PLI76_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HOI60605.1"
                     /translation="MMFFRYTRLLTAAALAALVLLMAAAGCTGTTAGGGAAGEEITVT
                     DGFGRSVTAPSPPESVVCSGSGCLRYLVYLQAQDLVVGVDDIEKKEQEAEGRPYALAY
                     GSKFKDLPLIGEFRGKDDPEKIISIGPDVVFKTGSTGTAYGTSAAEADKLEDKTGIPV
                     VAFPYGSLRDDAEKAEMYGGLRVMGEVLGKQDRAEEVIAYIDATMADLERRTGDIPAA
                     DQKSVYVGGISSAGAHGIISTEPAYPPFLWVHAKNVATGLGTAHADVAKEALVNWDPE
                     YIFIDAGTTQMESGGAVGELKNDAALAGLSAAKNGKVYGVLPYNFYNTNYETVLADAY
                     FVGKTLYPDRFADVDPVKKADEIYTFFVGKPVFGDLNGQYSNLGFAAIPL"
     gene            22866..24962
                     /locus_tag="PLI76_00850"
     CDS             22866..24962
                     /locus_tag="PLI76_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOI60606.1"
                     /translation="MPERMLTEPEGYGLLSAYGIPVPPHQVVTSAGDARAAAGRIGYP
                     VVMKIVSSDIIHKSDVGGVVTAIESPEGVEEAFRTIMQNGAARAPEAAITGIIVEKQV
                     PGGLEVLIGGKTDPSFGKVVTFGLGGKLVELLEDISIRVLPVTDDDIRTMVREIEGYR
                     LIRGYRGEPQKDEEALVRIIATMARQFAENPQIREFDLNPVILYEEGASVVDARIIVG
                     DTVGGEAARLSVGSPPDIFYPRSIAVIGASASPNKVGYSVLRNLLSFPGNLYPVNPSR
                     KELFGRTAYPSVLDIPGPVDWAVIAVPAPLVPGVMEECGKKGVRLAIIVTAGFREIGG
                     AGVALEEEVVEIARRHSVRIIGPNCLGVMMPHQGINATFDPVSPRSGDVAFISQSGAI
                     ITTVVDWSLPEEFGFSSVISVGNQADLGFEHYLRFAEQDPMTRSITLYVEEIQDGRSF
                     AQIVREVARRKPVVAVKSGSSRKGRAAASSHTGSLAGSYDVYVAAFRQAGVIPARTLR
                     DAFNLAELLASEGYPRGNRAIAVTSAGGFAVLASDYAEAHGVDMVDLPDEVLRELNAF
                     LPPFWNHANPMDIIGDADAARFAALFDVLIRHQDFWDIAFVIAVPTTLADPAHVANEI
                     VRFSRNTEKMVVGCMLGGDSIRSGLRILRSCRIPNFAELEDAFKAVGTILSVRTARSG
                     DLPRLPKPDWCAGGER"
     gene            25049..26419
                     /locus_tag="PLI76_00855"
     CDS             25049..26419
                     /locus_tag="PLI76_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOI60607.1"
                     /translation="MLFRKIKSDILAHNSYMIGADGEAAVIDPRRDCQVYTSVAGRRD
                     MKIAAIFETHKNEDYVSGSRELARPTGAEILHGAREEFGFGTPVREGETFEFGSLEIE
                     VLETSGHTVESISLLLRDRSVSDDPLMVFTGDALFAGDVGRTDLAGEDRRREMSEMLY
                     ESLHEKLLPLPDGVIVCPAHGAGSVCGGNISDREYTSIGFERATNRLLSMGKEEFVEY
                     KVNERLYEPPYFTMMRRLNKAGPPLIYNLPYLRPYTARELRSLLDRKAQVVDIRSPTS
                     FAGGHVPGTINIWRNGLPLYVGYYLNYEDPIVLVDDFNLGMGHVLRHFVRLGYDNIGG
                     YLAGGFPAWFKQGERIGRSGIWSPSDLAERLNDPSIYILDVRKITHREESGHIRGSHH
                     IYVGNLEERIKEIPEDKEIVVHCDAGFKGSLAASILKKHGFRHVANLLGGTIGWEAAN
                     YPLVRD"
     gene            complement(26572..27045)
                     /locus_tag="PLI76_00860"
     CDS             complement(26572..27045)
                     /locus_tag="PLI76_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HOI60608.1"
                     /translation="MEEVTLDRDDIAALSSDARITILKLLDTRTMTMSELAEGLGLAK
                     STVHEHLAILTDADLVTHANARKWRDYTLTKRGRRVLHPGKDHRIIFLFGTSLATVTA
                     GALLVASFLRGFAVQGGNVVRDPFLLCAGEVLLGATIVLWYLAVRCRRRTTPTPG"
     gene            complement(27172..27996)
                     /locus_tag="PLI76_00865"
     CDS             complement(27172..27996)
                     /locus_tag="PLI76_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60609.1"
                     /translation="MHPKTWIWLGIGVVLLLAAANLAILAAFPPGQQHVQEPVKRVDW
                     ILPFGPGFEYCGPYESPVIAYDGANETWHLRNVTAYNRTINLGDRYSLDPAHPEDIRY
                     TPDVAIEGGQVSLDFLIRNLAGEDCARADLAMTTGTTTLNTTTGSPEGNIPPGAATTL
                     PIDDLAAGEWREVRVTADLPRPGQEEVLYLAIDLTAPYTLTTPNGTPVDFDFRRETVT
                     LLGSNTSWKEEIDPGSTPDPDYNRARLPRGSATFIIMGIDRSEPIPPPTFPTLRAG"
     gene            28331..28762
                     /locus_tag="PLI76_00870"
     CDS             28331..28762
                     /locus_tag="PLI76_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60610.1"
                     /translation="MTPLHHEKSARLRRYLIAVAAAGFVLLGCCVVVSSYIAATIPPD
                     ALLLYEVTEETTQKGIVIRLDEEEFRNLPVLDAFLRDEEHNTEGEPYAPGDIRLVGSV
                     DYPEKIRQETIDAYIFDSETGRRKYFEYEGRYYRVGTIYRD"
     gene            28993..29694
                     /locus_tag="PLI76_00875"
     CDS             28993..29694
                     /locus_tag="PLI76_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60611.1"
                     /translation="MMKETKLLPILVVLLLAASVCPGFASASPVDEEYTMAAERVELT
                     DDEYIAAIEDLKSRENVVETRGYLPEFKDAQHRRSWYNFLDNLTGGMFDSVKPYLYPD
                     GPMINFGYDIEGYVRIGIVEGYPQDKAGVAVDEIYAMLNEEAEQLGIEDIPVKVEIVD
                     PDSLELDVRVDPTGGAAVSAALAEPADTTAYLGPNDAFSLFYNPVLLMCGLGAVIVLL
                     VGYFLVRRSRGKDAP"
     gene            29691..29897
                     /locus_tag="PLI76_00880"
     CDS             29691..29897
                     /locus_tag="PLI76_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60612.1"
                     /translation="MSVPVLPAIAFSLAWPLVLIALVGRLENEKRRPVASPGTGTIAI
                     ASGTIDTIYAGAPASPLASGMRPG"
     gene            29932..30720
                     /locus_tag="PLI76_00885"
     CDS             29932..30720
                     /locus_tag="PLI76_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60613.1"
                     /translation="MKSLKTDRTSKIAFILLLIAVGAFGILLLVDLLAIGPGYPPPKA
                     LQKWYIPQPRYEYAENGSVAANRTIDGNVVLLGDIEDGCPSLFPVLSRRCSHAMYVDT
                     MSGDRYLVVNWYFDDKTDLLQAETVLCIHLRASGNVSSAEMFLSREPELSRGEPVFSP
                     VAVTKFDNKTSSGYLAVVEKPLSPKHDDYFIVYYGVVGPAILPDHTATLENLMLRSYS
                     FHGARPLAPCSAREAATGSMHSVCEGMPLPPRAAESTAASNQRF"
     gene            30848..31660
                     /locus_tag="PLI76_00890"
     CDS             30848..31660
                     /locus_tag="PLI76_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-dependent protein kinase"
                     /protein_id="HOI60614.1"
                     /translation="MFSLTAEFPEMRDSYTLYRTVPVTVTEEQVREVAERFGLAGEPG
                     TMNKKTGEFLLIDASKDPEEQVSVYAHSGAVAYHIPDLELPTEAIRQPDLPTDAEAER
                     IALAFLEKTGMQSPGARIVKIGVGQKQEVWKAGESEPEVSCDVTKTVRFGRPLDGLPV
                     YGDEFTVILGDGGKVAGLVKTWREVTPDGNISLRSPEEAYEDLLAARTIRPRVGGEYG
                     RITIENVSIGYWMEPRGVVQDLVRPVYAFSGTAFRDGVPEPYIGYVFADAGG"
     gene            31669..32568
                     /locus_tag="PLI76_00895"
     CDS             31669..32568
                     /locus_tag="PLI76_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60615.1"
                     /translation="MMNGIKVRLFGLLLASLLIGAFFVPVISAGGESTNWLDTFDERV
                     TPRGMSTYNKPIVTKEPEQPGPEPAEIHPGLVLLGENATFPERESMTISGLPSGPTTH
                     VAVADPAYFSPVPSAGEVNLTNLPPLRKYDLVTADPAAFVAGAGSGSPVTLRLPDREF
                     VLDLEPVPGPVAEGARAFVKNESGTFEVALPHIWRFEGTVVGEPGSSASFTVGGDAIL
                     GTIRCGSTLLVISQAGTIEIDGEQRVVHVIYDERDITPRLRSPIFDCCVPPAGARDSP
                     DITENRITSLVKSLIDAIATGRT"
     gene            complement(33125..33265)
                     /locus_tag="PLI76_00900"
     CDS             complement(33125..33265)
                     /locus_tag="PLI76_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60616.1"
                     /translation="MTDRLVWCNRIKNGISLSAPNETLASAYLKKAEEALETMQTITA
                     RD"
     gene            complement(33262..33435)
                     /locus_tag="PLI76_00905"
     CDS             complement(33262..33435)
                     /locus_tag="PLI76_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60617.1"
                     /translation="MEEIERIGKTYGFPIHLTIYPREIFDRDLHTDPFLREVLRNHVL
                     IKGAEHFFEKAVR"
BASE COUNT         6447 a        10521 c        10697 g         6305 t
ORIGIN      
        1 gcgctcgatg agcaggatcg cctcgccccc ctcgaggggt tctggatcta ctcggccggc
       61 cccacggcag tccccctgaa cttctcgacc aaccccctcc tcccgcccgc ggagcgcacc
      121 ctcgttgccg gctggaacgc tatcgggacc accggcaaca ccccggcgac ggcgcgagac
      181 accctctact cggtcaaagg tcgttggacg accctgatcg ggtttgatgc caggaaccag
      241 gccttcgaga ccgggatcgt caacggcggg agcggcgcaa acaccgacac gcgatccgtc
      301 taccccggcc ggggctactg gctctccatg accgggccgg gcaccctcta tgccatagga
      361 gcatgatact gatgagacac acactctgga tccttgtact tctcttcgcc ctcatcgggg
      421 cagcgtccgc catccccgcc ctccccgcgg agttctcggg cacggtcacg atcgacgaaa
      481 acccggcgcc ggccgggacc gccatcaccg cccggatcgg cgaccgcgac tgcgggtcgc
      541 tcacgctcaa gacagccgga acctttggcg gagattccac gtttgataag cgcctgctcg
      601 tcaacggaga ggacaccgat ctcgggaagg cgatcacgtt cttcgtcgac gggatctccg
      661 ccgggacggc gacctacacc cccggcacct cgaccgacct tgctctcgcg gtgacgaaag
      721 gagatacgcc tgacagcgag tcctcagacg gcaccccccc cgggtcatcg ggtagtggaa
      781 gctcgagcgg cggttcattc acacctcctg cagcaccggc gacagagtac accgggagcg
      841 gcactctcga caccgatgcc cggggcgcgg tccaacacgc agcggtcatc gccgccgccg
      901 gtggagacgc ccgccttacc atcgcagagg gagttctggc acaggaccgc ttcgagagac
      961 cgctcgatac ggtgagcgtc gagaccgcct caccgggcga cgccacccgg gcagtccggt
     1021 gcggtcccga cggcgcgacc ttcgaccccg cgatcgaggt ctcgtttgcc ctcacaccgg
     1081 aggagtggga acaagccggg cagttcgtcg tccggtggta taacagcacg gccgacgtct
     1141 gggaacccct tgcgacgacg atccacgcct cgacccgcac agtcaccgca acgatatcgc
     1201 acttcaccct ggtcgcggtc tttgccgtgc cggccgatac cccggaggac accgtgacgg
     1261 caacccccgc cggctcggag accccgacct ccacgcctgc agccccggca accactgtgc
     1321 agcaggccgg tccatctctc ccggggctcg ccgggctcgc ggccctggcc ggggcggccc
     1381 tcctcatccg gaannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntcccggg
     1501 gctcgccggg ctcgcggccc tggccggggc ggccctcctc atccggaagc ggggttgatc
     1561 gccttccggg cgggggagag agcccctcac acccgggccg gggcggtgtc ccatggagat
     1621 gcggccgggc gcgacgccgg tggtgacgac cgatgccgga gcgaagatac tatcttcatc
     1681 cggcgcggat ctcttctcca ggagtgttga agatggacga gagaggaccc ggtgagggcc
     1741 gcggtcaccg cgggcggggc cgcccccggg ttcgccggat gatcggcgat ggaggagcgt
     1801 ttcgctgttt cggccccctc tgtgggcggc ccgacgaggt tgtcctcctc ctccccgagg
     1861 agatcgaggc cctgcggctc gtcgaccttg cggggttcga acaggaagag gccgcgatcg
     1921 cactcggcat atctcgaaag accctctggc gcgacctcca cgaggcgcgg aagaaagttg
     1981 ccgatgccct tgtgcacggg aaaacaatcc agatcgtcgg gtgcggccgc cggcggggag
     2041 agaggtgccc gggggatgaa tgaggcgaac ccgcagccca tgatctcatc ccgccattga
     2101 ggtggcgcgg gatccgctct attttagctc taaaaaatgt caggagcggt gtgcagccct
     2161 acaactgcac ctgcgagagg gcgtcgtaga cgatcttacg gtagatcgcc ggctgcgtcc
     2221 cgtactcctc ctgcgccttc cgtgagtcgt cggtggtccc gaacgcatcc agcctgtcca
     2281 gcgtctcgcg ggcggtatcc agcacccaga gatcgcgggt ctcgcggtcg acgatcgata
     2341 tcgactccgc acgaaccgag accaggcagg tgccgtccgg ggtcgtatag aggcttggtt
     2401 tcccgacgac cgcgacgaag gccggcggtt cgatccgggc gagctgctgc atcgcctcgg
     2461 gctggtagga tcccgccatg acaaagaagg tgcctgtggg atcgactatc cgcccgcggt
     2521 agaagatgtt ctggtcgccc tgccgcagct tctccgtgag ggttccgacg atgaagacgc
     2581 ggttgctccg gataccggtc gggaggatga cgtaggtcgg actcttctcg tcatcgccgt
     2641 ccttgaactg gtagcgggtc tcccggagct cggatgcaaa gaccctgcgg gccggctcac
     2701 gctcgaactg tgtctgaggc gtcacgccga ctcacctccg gcgcggttca ggagtgcagc
     2761 cagttcttcg ggcttgaacg caacgggcgt gcaggaactg gcaagcaatc ggccgccaaa
     2821 ctcactcccg ctacaggtgt agtaacgccc gagcaccgca ttcctgatgc gatagaagat
     2881 ctcgtccatg ccgatcgggt ttgactccgc gatcccgact gcctcgtcaa gggtgatccc
     2941 ggcgagtttc tcgacgagct cgcgctgcac caggacgttt cttgcacgta tgccgtcgtc
     3001 taagaccgcc ttcaggcgga gatcgtagcg gaagttgggc tggatctcat gcaccgggca
     3061 gtagttctgc cgggagaggg tccggttgca cccttcgacg gggcagcgct tgatcaggcc
     3121 cgagcccggg gcgatgtgga caagggcgcc ctggatctcg gtctcgctcc gcccgacctc
     3181 gatatcgccc tcatccgcga tgtacatcgc ggtgttcagg gtgagggaga gcctgccgtt
     3241 gtactcatct accgtggcgt agaagatatt gtagacggcg tcgagatcca gtttatcctt
     3301 cccctcctcg ttccaggtga cgaacttgat ggtgccggtc tcgtcgccga ggagccccgt
     3361 ctggagcatc cgctcgtggg agacctccca gtcctgaacg accttggccc gcacgcttcc
     3421 cacgcccggt ttcagctccg ctatcggcgt gatcgacggg agaagcgggg catccttctc
     3481 catccgggag atggtggtgc ccgagtggat cttcaggttc ggtgcgcctt tgtactcatc
     3541 gacgaccgcg gattcgatcc ggtaccagtg accgtactcc attgcagggg cgtttgcccg
     3601 ggtccaggtc acgaagcgca tggcgccgct tgagtcggca acaatcccgc tctgcgcgat
     3661 cgagggggag agcgccgggg tcagggcaac gatctttccc tcgatggtca cccattcgcc
     3721 gggaacgata tcgctgatcg ggcgaagttc cgtggaggtc cccccgatgt tggtgacctt
     3781 gtactcgcgg gcgagatcgc ccgtcaccgt ccgttcggcc tcggcggcgt taacaccgaa
     3841 ctcctcgacg aggcgccgaa ggcgtgactc gatcttctgt cgatcgggct gtgcaccttt
     3901 tgcttcaagt ttccgggaga ttctttctgt tacctcggat aggttcatgc atacatctcc
     3961 agtctatact tcacctcgcg agggtattaa ggctcggcgt gcggggtgaa agtgaaactt
     4021 atggctcctc gccatggtgc cgggcgcgac cccggggtag gactgcaaca gtgatgggtc
     4081 ggccgcggat atgccgcccg aaaagattgc ccgtgcccga tgggagcggc agaatatccc
     4141 gatccgaagt ctgcatccgg atcaggggat atccgccctg gctcttgaac gtatatatac
     4201 cggcagggat aacatcagat gtatggcatt aactgcggac agtacaatag cagaactcct
     4261 tcgggagaaa cccgagtcgg tacaggtgct tttccggttc ggcatgggtt gccttggctg
     4321 cgccatcgca aataacgaga ccatccggga agccgcccag gtccacggca tccccctcga
     4381 agagatgctc tccgccctcg gcgttgccga agcgtgaatc ccctcttaca acctttcccg
     4441 gaggcgcttg agctcggcga gcgtggcctc gggattcctt ttcacgtaat ccgcgatcgc
     4501 agggacgtgc aggagcgtac agccggcaca cccccagacc ctgccgccgt ttgaactctc
     4561 gacccactcc ccgagctcct cgtcggcaca ggggtagcag gggcagtagc agtagtcgca
     4621 ccgctggccg gggaagtggc aggggtagta ggggcatccc tccttcaccc actccaccca
     4681 gtgatcgccg ccgtaccggc tgtagatgaa gaacgacggt ccgctccgcc ggacctttcc
     4741 ctggtggcgc gcgagggctt ccggcacgcc gtagaggatg gccgcgtgga cccgcctccc
     4801 ggcttcggtc agcgtcccgg cataggtatg cgcgccgggg acgtcacgtt cacaggcgac
     4861 gacgaccgca tccgtcgttg tgccggggaa atcatagcca agatcgtgga gcgcccgggc
     4921 tttcgccccg gtcgccgtga cgatcgtctc aaggagcgcg gcgtcgcaga ccccctcgcg
     4981 gctgtggacg acgatgttga tggtgtgcgg ggcgttgtcg acgggaggcg tcgggttcgt
     5041 caccccggcg gtgatgaaga ccgtaatgaa gtcgtactgg agcacgcaga ggtgccgcat
     5101 ctcgacggcg gtcaggagcc cgaaatactc gtggaagaga ccgtgccggg cggcgagaag
     5161 ttcgaggtag cgctgcggtt cgtcgcaaaa atcgcgggac accgtatggt tcaggactgt
     5221 cgtgacgtca gcgataccac cgttgatgcc ggtgctcgcc gcccggaacc ggccgcggag
     5281 gaagagcgta tcgtccctga cgaaatgcct catacggcag agtagcggac ccggtctttc
     5341 agctcctgct gtttgcccgc attccagcct gcaacgtcct ggagatatcc ggtaaccctg
     5401 ctgatctgga cgacatcgtg gctcccgcac tcagggcaga ccgcatggcc gcagagcggg
     5461 cagtactcga tgctctcgac gatggagtgg tggcagtcac agtgatcgag cgggcacatg
     5521 atctcaagcg cagtctcgcc gcagtgcggg cagggggtct cgtccacgac gaagtggcag
     5581 gtgtggcact tgtagcgccg ctcgccggcg ggtatatcgt tgaggctccg gtacttctcc
     5641 accagtttct gctgttctgg gctccagttc atgatacgag tatctgcagg aaaatatatt
     5701 aacccttcag atgcggaacg gtccgcatcc gcctcagacc actcataggg ttcgtcatat
     5761 cccggaggga tcggaacgga gaattcatca tcgacggatg cgggagaacc gttcggcctc
     5821 agcgcccggg tccgccgcct cgtatatgct cctcccgacg atgacgccgt cgaccagccg
     5881 ggccaccgta tcgaggtcgc cgccctgcgc ccccacgccc ggggagtaga tcgttctttc
     5941 gccgacgatc tcgcggagcc gaccgatccg gtcgggccgg gtggcggggg cgatgatacc
     6001 gtcggcccgg caggcgaccg cgagcgcggc gaggtgttcg gccacgccgc cgtggaagta
     6061 ctcggtcgca ccgggatggc tcatctccgc gacgatgtag gcggctccac cgtggttgtg
     6121 cgccacctcg acgcaggccc gcgccgcgtc acctcccacg aacccgtggg cgatgacggc
     6181 atcaaacccg gccgaaaaga ccgcctcgca gatgaggcgg ttggtgttcg gtatatctgc
     6241 gaccttgaag tccgcgatga gcgggaggcc gacaccagag agatccccga cgatagagag
     6301 gccggccgcg aggacgagcg ggtagccgac cttgatcgca tcgatgaagg gtgcgcagga
     6361 ctctgcgacc gccaccgccc gcctccggtc ggtgacgtca agagagagta tgagttcggt
     6421 catccttcca gcctctcgag ggttgcggcc acaaggagcg gcaggctgat ggtcgcatcc
     6481 ccgtagaccg tcacggcggt ggcctctccg gtgagtttgc cccacgaccg cgcttcgtcg
     6541 agcgtcgcgc ccgagagtcc gccgaggtcg ggccggtcgc cggtgagctg gaccgcgtaa
     6601 tcaaacccgc tctcggtcat cagtttgctc tgaaggatgt agttcttcgg gacgccgccc
     6661 ccgacaagga ttgccccggc ccgttcggcc tcaaaacacc ggtcgaggag ggcgggcata
     6721 tctccaaacg cgctgaccgt gaccttatgc gtctgggaaa aaagccagta ctgcagcccg
     6781 atcatcgagt cctggattgc cgggcagtag accggcaccc cggcctttgc cgcttcggcg
     6841 aggatcccgg tatcgagcct ctttccgatc cggttgagga gttcggcaat cgagaccgtc
     6901 gaaccctccg gcagggagga gtagacatcc tgcatgaact cctcgaacct gatgaacgcc
     6961 tcgttcggga ggaagacgtc atagatccgg ttgatccctt cctcgcagag ttcggcgtcg
     7021 gagacgtcgg cgaccccgtg gtagtggtgg cagccgatcg cctcgatgat gtcgtgggtg
     7081 aggtttgcac ccgtcgatac caggatatcg atgtgccggc gccggatgag atcggcgacg
     7141 atgcccccca tcccgccggg aaccatggcg cccgagaggc cgaagaactt cagcgcctcc
     7201 tcgtcgcgga gcatcctctc gtatatgttg accgcctgcc acagggatcc tccgttgtag
     7261 gcccccgctc ttccgagttc gtcgacgagc tcgccgaccg tcataccggg gcgcacacgc
     7321 gcctgttgta ccgcattccc atacttgaag gtcatgattc cgttacacca gttcgtgaga
     7381 tggtattggc attcatgcac aataacacct cgtctcctcc ctgtggcaac gtccgacgac
     7441 caaaaatgta tggagatgtt gcaggaaccc atagatcggg tccccgctgc cagacagggg
     7501 atgactggtt ggccgacacg ggatgatccc gctcgtcccg ccgtgtcgga gatccggata
     7561 tgagccggat agtctcgtga gagagcggac tttcgggccg ggagaccgga ggatctccgg
     7621 agggatgcct tcgtctctgc tccacgcgta ctcgaccgcg gttctgtgca gcctgccgct
     7681 gaaccccatc tgccgtgggt cgcaccgtca tgggcagaca ggtcaggaca cgtgcaccat
     7741 caccgctgat ggtcggcacg ggatcaccag ggataagtta actcacaaac attataaact
     7801 ttattttgta aagccagttt gattgacgag gcaactccgc ccatggcgtc tccccggggc
     7861 acggccgccc gtggaggtca tggccggccg gattgaagga gatcttcctc ccatgccggg
     7921 acaccgcgca ccagcggccg ggagagatgc cggcgggcga cgggggggac ctcgtaccac
     7981 cccggccgga tccggcgctc ccacctctcc acatccggtt cgcggctgcg cccgccgcag
     8041 acccggcact tatagccctt cgcagtcccg gcgctcgtca tccttctcgc gcaggccggg
     8101 cagaccggcg ggcggatgcg aaccagagcg gcaaggtggg agaccccgag cttctcgagg
     8161 ttgaggctcc cccccttgta gctccccgcg acagccactc tatccccggg gacgagggct
     8221 cgtacgacgt cgcgaaaccc cctggtcggc tcgtaggcca tacagcgaac ctcgacgccg
     8281 ccgtcctcga ggaggaacga gacgtggccg ccgggtccgg tggccggcag tccggcgacc
     8341 gcgccccgca ccaggtagga ccgcccctcc cggagttctc cgatcgcccc cggcagcagg
     8401 tgagcgtccg tcccctggtt ggtggtgtag acctgctcgc aggcaggctc ctccgagcgg
     8461 acgtacgacc gcgcttcgcg gacggctgcc gggctctctc cgcggatgcc gaagaggacc
     8521 ggatcgggcg tgtggggcac gcagacgacg gcatcgttct ttcggtccac cgtatcccag
     8581 gtgcgggggt aggtcgtctc atcggccaga aagaggctcg cgcggtccac ctgccgcggg
     8641 gtaccccacg cttcccgctt tcggtaggcg agcagctcgt acgtccggtc gaagaagacg
     8701 ctcgcgactg ccgcggtcgc cccgataagg ccgcgccggt tcttataacc gcgataacgg
     8761 gctccgatcg attcgagcac gtcgaccgcc tcgtccacgg tgcagaaatc ccggagcacc
     8821 gcgtagtaaa aatccggcgg cggccggacg gcggcgacca ccacgcccgg gttcgtctcc
     8881 ggggcatcaa actccgcgag ttcttccacg cacgcgcagg caagcgcaaa caccgcctct
     8941 gcatcacctt cggcctcgat gcagaccgct gcgttccccc gggtcttgtg gatgacgttc
     9001 gggttcaatc ggaccagccg ggccccggtg atggggatcc cacgctcttt caaccgccgc
     9061 accagcaccg ccccgatgta ggtggtgcac atcccggcag gagaatccgt atcgtcgatc
     9121 ccgatccaca tacgctataa tttatatctt ctccgactat atcgattctt acgggttatg
     9181 tcgcaggatc gccttcctca gatggtcatc agcatcatgc tcctcgcgga cttcgatatc
     9241 tcggagcggt gcaacatccg tccgcggagt ttcgacctta tcgcgaagaa aggcgacacc
     9301 ctcgtcatca tcaaagtcgc ctcccagatc gacagcgtga ccgccgatat cgcctgggac
     9361 ctcaacctga tcgcccggta cctccaggcc accccgctca tcgtcgggga gcgcgctcgc
     9421 gacgcggaac tcgagcgcgg cgtcgtctat atccgttacg gcctctttgc cctgagctcc
     9481 gagacgctct acgactactt tgcagaaggg atatcgccga tggtctatgc atccccggga
     9541 ggcctctacg tgaagatcaa gggcgatctc ctccgcgaag tccgcgagcg ctcccggatg
     9601 tcgcttgggg atctcgcgtc gcacctcggg gtctcccgcc ggaccatcag caagtacgag
     9661 agcggcatgg gcaccacgct cgatgtcgcg atacggctgg aggaactctt cagcgccccg
     9721 ctcgtcgaga cgatcgaact cgtcggctac cgcacccccg agcccgaaaa tcccccgaag
     9781 tccacagttg gcgacgtctt catcgatctc gaacgcatgg ggatggagat ccacgcgatg
     9841 cggcaggccc cgttccaggc tctggcgctc tttgaccggc acaccatcct gacggcctac
     9901 ggcaccaccc agaaggttgt aaagcgtgct tccctcatcg gcaatatatc ccagatcacg
     9961 aagacatttg ccatgtgcgt cgtcacggat tacaacaagc agaagaagat aggtaaaacg
    10021 ctccttatcg gcgaagagca tctccacact ctcgaagatg gctcagaact catcgatatg
    10081 ataaacgagt gaatactatt atatagaact acaaacctac ctttctatcg cttaacaacg
    10141 gtgataccta tgtcaagtct tggaggacaa ccaatcctta ttctgaaaga gggtagccag
    10201 cgtacccgcg gtcgtgacgc acagtcaggc aacatcgccg ccgcaaaggc cgtcgcaaac
    10261 gctgtacgga caacgcttgg cccgaagggt atggacaaga tgctcgtcga caccatcggc
    10321 gacgtcgtca tcacaaacga tggcgtgacc atcttaaaag agatggacat cgagcacccc
    10381 gccgcaaaga tgatggtcga gatcgcaaag acccaggacg acgaggtcgg cgacggcacc
    10441 acgaccgccg tggtgatcgc aggcgagctc ctgaagcgcg ccgaggatct cctcgatcag
    10501 gatgtgcacc ccacggtcat cgcccacggc taccggatgg ccgcagacag agcgcaggtt
    10561 atcctcgacg agattgccgt cgacgtcaaa cccgacgata tggcgatgct caagaagctt
    10621 gccgataccg cgatgaccgg caagggcgcc gaggccgcca aagagaagat caccgacctc
    10681 gtcgtcaggg cgatcacgat ggttgccgat gccgacggca ccgtcgacac cgagttcgtc
    10741 aaggtcgaga agaaggtcgg cggatccatc gaggagtccg agatcgtcga gggcatgatc
    10801 atcgataagg agcgcgtcca ccccgcgatg ccccgcgtcg tcaagaacgc gaagatcctg
    10861 ctcctgaacg ccgccgtcga gttcaagaag accgaggtcg acgccgagat cagcatcacg
    10921 agccccgacc agctccagat gttcctcgac gaagaggagc ggatgatcaa gggcatcgtc
    10981 gacaagatca tcgcgagcgg tgcaaacgtt ctcttctgcc agaagggtat cgacgacatc
    11041 gcccagcact accttgcaaa ggccgggatc ttttccgtcc ggcgcgtcaa gaagagcgac
    11101 atggagaaac tcgcccgtgc gaccggcggc gcaatcgtca gctccatcga cgccatcgcc
    11161 cccgaggagc tcggtaaggc aggcactgtc gaggagaaga aggtctccgg cgaggagatg
    11221 accttcgtga ccgggtgcga gaacccgaag gctgtctcga tcatcatccg cggcggcacc
    11281 gagcatgtcg tcgccgaact cgaccgtgcc atcgaggatg ccctcagggt cgtcagcgtg
    11341 gccgtcgaag ataagaaact cgtcgccggc ggcggcgcgc ccgagatcga actctcgctc
    11401 aggctccgcg aatacgctgc aaccgtcggc ggccgcgccc agctcgccat tgaagcattt
    11461 gcaaacgccc tcgagatcat cccgaggacg cttgccgaga acgcgggcct cgacccgatc
    11521 gacatgctcg ttgcgctgcg cgcatcccac gagaagggcg gtgcaaacgg gaagtacatg
    11581 ggcctcgatg tcttcaacgc cgtctccggg gatatgctca aggccggcgt cgtcgaaccc
    11641 ctccgggtaa agactcaggc ggtctccagc gccgccgagg cagccgtcat gatcctccgg
    11701 atcgacgacg tcatcgccgc gtccaagtcc gccggcccct ctcctgagga gatggctgcc
    11761 atgggcggcg gtatgggtgg cggcatgggc ggcatgggca tgcccccgat gtaaatcacc
    11821 gtaccgttcc acgatacgat ccggggccaa gatacggccc cggcattctc ttttttcttc
    11881 gctgccggga tatcgcgccc ttcgctgcaa tcaggcatgc cgccgctttc tccggggttt
    11941 tctgggcaaa acaaccgata aaaccccatt tatcacatca aaatcgctca cactaaatac
    12001 ctgcaggtat acctttttga aagaatgttc tgtgccctgg gggaggcgca aaacgcggag
    12061 tatgtgcccg gatgacgttg aggggatcgg tgcggcaggc tctcccgttc acgatctacc
    12121 tcctgcttgc cgtcctgctc gcgatggcgc ccgtcatcta cctgatatcg tttgtcgggt
    12181 atgccgatgt ccggcaggct ctcgtggcgg atactggaga actccggggc cagaccgagt
    12241 acgggatcgt cctcgcggta aaccttgtcg atgccgggct gaaactcttt gacgataccc
    12301 tgaaccgcga gatgcaggag ggattcggtc cgttcgccgt tgagtacgaa cgggcggggc
    12361 gggatccggg cgtcatggat cttgcccggg tcaaggagga gctcgggggc cggatggacc
    12421 tttacgtcat caacgagaac ggtatcatcg agtacaccac ctatccgccc gagctcgggt
    12481 acgacttcaa ggagatccct gccttctacg accgcataac ggatatccga ctcaacggcg
    12541 ctttctcggc cgacaggatc gtccccgagc tcgccaccgg gatgctccga aagtatgcct
    12601 accaaccatc tcccgaccgc cgctacctct tcgagctcgg gcaggtggtg tcggagtccg
    12661 gagggcagcg catggcgctg agctatgcgg atgcaatccg ggaggtagcg gatgaaaacc
    12721 cgggcgtcag cgagatccgt gtcttcgact gcctgggcga gcggatcgtg ggtgcggcgc
    12781 atcccgacga tgatcgccga ctgggcatgg ttcggaaggc gtaccgggag aggaccatgc
    12841 ttgagacgga gaacgtcgac acaggcgagc tgatccggta cctcttcgtc gatagaacgg
    12901 atagcgttca cgcatcagac atgagcgtgg tcgtcgagct gacctacaac acgaaattcg
    12961 tggaagatgc gatcgcgggt atgtttgccc gacatactca aatcctgctc atcgggcttt
    13021 cgctcatcgc ctgcctctct gccctcgcca tacgctccct gacgcggccg gtacgcatcc
    13081 tcgtcgagga tgtcgacgct gtcgcccgcg gcgacctgga ccgccccata cgggtgagcg
    13141 ggagcgagga gttcgtgcat cttggaggga gcgtatcgac catgatcgca tccttaaaag
    13201 agatcctgca aagactccag gagtcggaag agaagatcat ccggcacaac cgcaccctcg
    13261 aggatgaggt cggggcacgg acggccgccc tcgaggaatc caaccggacg gcgtcgctcc
    13321 tgctcgacat catgggccac gatatcaata acgcaaacaa catcgcaaac ctctactccg
    13381 acatcctcct caccgacctt gagggtgaac ctgaagcgga actgctccgg aaggcaaagg
    13441 cgggacttgc aaagagcatc gggatcgtgc gcaacgtcaa caccatccag gagatccagg
    13501 ggaaaccagg ggctatcgga aagatcgacc ttgaccgaat cgtcagatcc gagatcaggt
    13561 cactccccga cctccgtata acctacaacg gcaccacagc tgcagtcatc gccgacgacc
    13621 tcctctcaga ggtcttttcg aacttgctcg ggaacgcggc gaagttcggt gggccggaca
    13681 tcgcaatcgc catccggatc gaggagcacg aagacgatct catcatatcc atcgaggata
    13741 ccgggccggg tgtcccggat gccgtaaaac cccgggtctt caaccgcctg gcccggggga
    13801 acggagaggc cgcggggacc gggctcgggc tctacatctg ccggatgctc attgcgcggt
    13861 atggggggag gatatgggtc gatgaccggg tggcggggca ccccgaatcc ggcacggccg
    13921 tccggttcac cctccggaag gccgatgagg gaggtagccc atgacggcgg tggatggcgg
    13981 caggtggcat cgccgtcttg gcacacacct gctgctcgcc atgatcctga tcacgctccc
    14041 cgtgatcggg ttgatctcgg ccctggactg caatgaggtc gtgggggccc tgatggccgg
    14101 ggacgagctg ctccgtgagc agacggagaa gagcgttgtc cggtcggtgc gcctcgcgga
    14161 tgcggggctg aaactcttcg acggcacact ggactcccgg atggcggagg ggtttgaccc
    14221 ggttctcgcg gggtacgagc gcgccgggag ggaccccggg gccatggacc ttgcccggat
    14281 ccgcgaggag ctcggggagg agatggacat ctacgtcatc aacgagtcgg gcatcgtcga
    14341 gtacaccacc taccctccgg acatcggcct cgatttccgg aaacttccca ctttttatga
    14401 caggataaca gatatccgcc tcagcgatgt attcgtggcc gatcgcgtcg tgacggagcc
    14461 ggcaggcggg atattgcgga agtacgccta ccagccctct cccgaccacc gctacctctt
    14521 cgaactcggg ctcgtctgca atgcgaccga ggccggccgt tacgccccgg actaccgggc
    14581 cctgagggaa gacctgatgc ggttgaaccc cgcgctcaaa gaggtccgga tctacgacag
    14641 ttacgggcgg ctcgtcaacg tgaccggatc ggagagcccg gtcgtgtccg tggcggtgga
    14701 tccggccgcc attgctatct ttgaggggag ggaaggcagg acgctcgcga ataggactgt
    14761 cgggactata acccggtata tattcatcga cctctccgat cccgccagcc cctcggacaa
    14821 gagcctggtc gtcgagctga cctacaccac cactctcctc gacgcccgga tagcggacgt
    14881 gcagctcggt catatcacca tcgttctctt tgcgagcctc gccgcctgct gcatcgcgat
    14941 cccggtctcg cggcggatca cccgcccggt gcaggagatc gtcgatgacg ccgacctgat
    15001 cgccggcgga gatctcgacc accggatccg ggtctctacg gggacagaat ttgctcgcct
    15061 ctcggagagc atcggcatca tggtcgactc cctcaagggg aacatccggc gcctccagga
    15121 gtcggaggag gctttacggc agtatgatgc ccgtctcgag gatatggtca gggagcggac
    15181 ggttgagctg gaggagtccg gccggacggc aaacctcttc ctggacatca tggtccacga
    15241 cataaacaac gcaaacgcca tcgccatcgg gtatacgcag ttcctggtcg atgcgctcga
    15301 gggcgagcgt aaggagatgg cgcaaaagat gctctcgggg cttgagcaga gttcggggat
    15361 cgtcgggcgc gtcgccaccc tccggaaggc ccgggagagc gtgacgacgc tcacacccat
    15421 ggatctcgac cgggtgatac ggacagaggt tgccggccac cctgcggccc ggatacggta
    15481 cgagggccgt ccggtcgacg tgcttgccga tgacctcctt cccgaagtct ttgcaaacct
    15541 catcggcaac gcggcgaagt tcggcggacc ggatgtcgca atcgccattc gggtcgagga
    15601 gcgtgaaggg gaggtcacgg tcacggtcga ggataccgga cccggcatcc ccgatgcgat
    15661 gaaagagagc atcttcggcc gtttcaggaa aggggaggag tcgcttgccg gagccggact
    15721 cgggctctac atctgccgaa cgcttattgc gcggtatagg gggaaaatct gggcggaaga
    15781 ccgggttacc gggcatcccg agtccggcac ggccatccgg tttaccctcc ggacggccgg
    15841 gtaatcttac ccgatgatct cctgtacccg cccgacctgc ccatcgcgga gccggacctt
    15901 gatcccgtga gggtggtagg acgagtttgt caggatctcg gcgacaacgc cccgggtgcg
    15961 cttcccggtc ttctggtcgc gtttgagcac gacgtccacc gtcatgccgg ggtggacggc
    16021 ggcgcggttc tgcccgttca tgggcggaga tgaggattca ctcttcccca tcgctctctt
    16081 ccgtcttatc cccgttctgc ccggggacag cggtcttcat ctgcgggaag aggaggactt
    16141 ccttgatcga gtccttgccg gtgaggagca tcaccaggcg gtcaatccct atcccgacgc
    16201 cgcccgtcgg gggcataccg tagccgagcg cgttgatgaa gtcgtagtcg atcatctgcg
    16261 cttcgagatc gccctttcgg cgcctggcat cctggagctc gagccgggcc ttctggtcga
    16321 gtgggtcgtt taactccgag aacccgtttg ccatctccat cccggcgatg aagagttcga
    16381 accgctcggt catcccctcc ttcgtccgat gtttctttgc gagcggcgag ttctcgaccg
    16441 ggaagtcgta gacgaacgtc ggctgtatga ggttcttctc gccgaagtgc tcgaagaaga
    16501 ggaccaggta ctctcccggg gtcgcggcgc tctcacagcc ctcgacgcag tgcgcaagac
    16561 cgaacgcacg gagttcctca aggctcatcg cccggacgtc gatgccggcg tactcccgca
    16621 ccgcttcctc catggtgagg cggcgccagg ggagggagag gtcgagatcg tggcccgcaa
    16681 acgggacgat cgcacttttg cacaccttct ccgcgaggaa cgagaggatc tcctcggtga
    16741 ggtccatcat gtcgttgtag tcgcggtagg cctcgtagat ctccaccatc gagaactcgg
    16801 ggttgtggtt ggtgtcgatg tcctcgttcc ggaagttctt tgcaatctca aatactttct
    16861 cgaacccgcc gacgacaagg cgcttgaggt agagttccgg cgcgatccgg aggtagagtt
    16921 tctggtcgag ggcgttatgg tgggtggtga acggccgggc gttcgcgccg ccgtagaccg
    16981 gctgcagggt gggagtctcg aactccaggt agtcccgctc aaagaggaac tgccggagga
    17041 gcgatatgat cctgctccgg gtccggaacg tctcgcggct ctcttcgttc atgatcaggt
    17101 cgatgtagcg ctgccgatag cgggtctcga cgttcttgag cccgtggaac ttctcgggga
    17161 gggggcagat cgattttgcg aggagttcga accggtcgac ccagatggtg agctccccca
    17221 tcttcgtccg gaaggcgtgg ccgacgacgc cgacgatgtc tcccgcgtcg acgtactgcc
    17281 ggaagaggtc gaactgctct tctccgaggt cgttcttgcg aagatagagt tgtatcctcc
    17341 cgtctgagtc tcccatgtcg gcgaagagcg tcttgccgtg ctcgcggacg atgtagatcc
    17401 tgcccgcggt gctgacctct tcggcgcttt tttcgtgccc gatcgtggag aaccgctctt
    17461 tgatattcct gacggtatcc ctgcgctcga aggtgtaggg gtagaccgtc accccgcgct
    17521 cgagcagatc ccgtatctta tcgatctttg ttgggtcaaa acagaggtga tccatatcgc
    17581 tcattgtgta aacagctcca tttgtcatgc ggcccgattt cacatcccaa acccgggttt
    17641 gccgcttttc cgggaagaaa gagccgcaat ctcattgata aatcctacat atacttccgc
    17701 tccggtgcta ttaatctttg agggagggtg gatcgaggcg gcgcaggcac ccgggctgca
    17761 gcccggaccc cgtcgcaggc cgcctcccgc ggcattacgc acacgtgaga gggccggccg
    17821 ctcccggtct gcaggcaatg ggcccggccg gcgaacccct ccaggtccgc cgtctgccgg
    17881 cggcaaaacg caatgcataa gaaggttata cgagttatcg tcaatagtgt gggcatggtg
    17941 cgtgagacgg gaagagaggt agcgatagag aggcttgccg ccgtcatcag cgaatgccgg
    18001 aagtgccccc tctgggagaa cacccaccac gcggttccgg gggatggcga catcagggcc
    18061 ggcctcatgc tgatcggcga ggcaccgggg aagcaggaag acctcaccgg gaggccgttc
    18121 gtgggacggg caggaaaact tctcgacggc ctcctctccg ggatcggtct tacccgggac
    18181 gatgtcttca tcgcaaacat cgtcaaacac cggccgcccg gaaaccgcga tccccgtgac
    18241 gaggagatac gggcctgcac cccgtatctt gaagagcaga tccggatcat cgagccgggg
    18301 gtgatcgtga cgctcggtcg ccactcgagt cgctacatcc tctccttcct cgccatcgag
    18361 tttgatcgga tcacggatat ccgcgggacc gtctaccccg gctccctctt cggccgcccg
    18421 gttcgcctca tccccaccct ccaccctgcc gccgcgctct ataacccggc ataccgggag
    18481 gccctggcag aggatttcgc gatcgccaga cgtgaactcg caaggacggg agggactgca
    18541 gaactggatt gaacggccat gacgagggag aagtcatacg gggcggtggt tttccggcgg
    18601 gatgcggatc tccagtacct catattgcag tacggggccg ggcactggga cctcgtgaag
    18661 gggcacggca aacgtggcga gagcgagaag gagacggtac tccgcgaact tgcggaggag
    18721 accgggatcg cccaggccac gttcatcccg gggttccgtg aggagatcca ctacttcttc
    18781 cagcgccggg ggcgcacggt ctacaaggag gtcgtctact acctgatcga gaccccggaa
    18841 gagagagtgc tgctctcgga cgagcatatc gcctaccagt ggctccctta cggcgaggcg
    18901 ctccaggcga tcaccttcgg gaactcaaaa cgagtcgtca gcgaggcgca cgaacacctg
    18961 acgatgctgc agtcccggga gagcgggtga ccgcgagacc cagtacctca tcgttccatt
    19021 ctatcttccg gaatctcgct tgcctctctc ccgaggaagt aactcacgac ggtcctgatc
    19081 gcgacgatcg acccgagggc gatcagatcc tcaaacgtcg gtttgaggat cgtcttcaag
    19141 acgtcggcgg cgacgaagaa ctcaaggccg atcaggatgc gcgtggtgaa taccccccgt
    19201 atatcgtggt aactcctctc acgtatgcgc gtctcccgcg cgaggagttc gacgatcgcg
    19261 atcatcgctc cgtagacgat gaaaaacgct ccaaacgccc cgatgaccag cccgacgacc
    19321 ccgatgaatg tctcgagcgc catgcccggc gggtgtatcg gcagcgggtg gacttataag
    19381 tatagcaatc gccggtatcc gcgcgggaga ggacagaggt ggtaagagag gcccgcgcac
    19441 gagacatcgg ggcggcgtac ggcccggggc caggcatcac cccgatccgg gccacggtgc
    19501 gtatcagcag gtgctccccg acgcaagggg gacgacgaca cggaatccgt tatagcgctc
    19561 gaccgtcacg gggacaccgt agaccgcctc gatggtctcg ggcgtcacca cctcgtgccc
    19621 gcccgcggca tggatgacgc cctcctttaa gaagatgaac cggtcggcgt agcggagcgc
    19681 catgttcagg tcgtgcatcg tcatcaccgc cgcgacatcg tgatcggtca ccacgcttct
    19741 cacaatcccg aggatctcaa gctggttctt gaggtcgagg ctgctcgtcg gttcgtcgag
    19801 gaggagcacc cggggctcct gcacgagcgc ccgggcgatg ctcaccttct ggagctcgcc
    19861 gccgctcatc tcgtcgatat accggagcga gagagcctcg aggtcaagca tccggatagc
    19921 cgcctcgacg atgcggaggt ccgcctccgt gacgtcccac cggatatgcg gccgccggcc
    19981 aaggaggatg gcatcgaacg cggtcaacct gcctgcctcg caccgctgcg gcacgtaacc
    20041 caccttccgc gcaatctcca tccggtcggc cctgaggaga tcgatctcat cgacgaagat
    20101 ggatccggcc ttcggtttca gtatggcgtt catgcacttc aagagcgtcg tcttcccgac
    20161 gccgttcggt ccaaggatcg cgaggatctg gtgggggcgc acgccgaacg tgacctccct
    20221 gatgaccggg gcgctcctgt aggtgaacgc cacgccgtcg acttcgagga tcatcgccgg
    20281 tacccccgga ggagcaggta gatgaagacc ggtgcgccga ggaacgccgt gagcacggcg
    20341 accgggagga tgtagggcgc aacgatgatc cgggcgacgg tatccgaacc gaggaggagg
    20401 atcccgcccg ccacgcagga gcccggtatc aggtagcgct ggtcgtcgcc gatgagcctc
    20461 cgcaccatgt gcgggcagac cagccccacg aacccgatga caccgaggaa cgagacgatg
    20521 acggcggaga cgagcgccgc gacgaccata ccgatgttgc gcaccgcttc gacactgacg
    20581 ccgagccctt ttgccgtctc gtcgccggca tcgatcgcgt tgtagttcca gcggttcgca
    20641 aggaagaaga tgctggccgc cgcgacgacg aaagtcatga taccgagctc ctgccagccg
    20701 gcccggctga cgtcgccgaa ggtccagaag acgaccgcag cgagctgggt gtcgctcgca
    20761 aagaactgga ggaacatcgt ccctgccgtg aagagggagg agagggcgac gccggcgagc
    20821 accatcacct ccggcgacgc cccgcgcacc cgggatatga tcaggatcac cgccgtcgca
    20881 aggaggcaga agatgaacgc cacgaccgtc gtcaggtagg ggttgttcag ggtgacggca
    20941 ttggcaaccg atgaatgcat ctgcccggtg ccgaggagga tgaccgagac ggccgcaccg
    21001 aacgcgccgg cattggatat gccgagcgtg aacggcgatc cgagcgggtt acggaggatc
    21061 gactgcatgg cgacccccgc caccgagagc ccgacaccgg cgacgatcgc ggcgagcgcc
    21121 tgggggaggc ggatgttcca gacgatccgg tcgacgttgg atacgctcat agtggcaccg
    21181 ggattgagaa acacgaagat ccggtcggcc gtgccgttca cgatggagag gaagacgtcg
    21241 tagatgggaa cgccggccgc gccgaccgat atggagacca cgaggaggat gaagaggagg
    21301 gcgatccccc cgagtatcca gaggtgcttt ctcctcacgt acccgagata ttcccgggga
    21361 actgtcccgt cagcgaagtg catctcgtct tatcctccgt atatcgcatt tacagcggga
    21421 tcgcggcaaa cccgaggttg ctgtactggc cgttcaggtc gccaaagacc ggtttgccga
    21481 cgaagaaggt gtagatctca tctgccttct ttaccgggtc gacgtcggcg aaccgatcgg
    21541 ggtagagggt ctttccgaca aagtaggcgt cggcaagcac ggtctcgtag ttcgtgttgt
    21601 agaagttgta cgggagtacg ccgtagacct tgccgttctt tgctgcagag aggcccgcga
    21661 gcgccgcatc gttcttcagt tcgccgaccg cgccgccgct ctccatctgg gtggtgccgg
    21721 cgtcgatgaa gatgtactcg gggtcccagt tgacgagcgc ctcttttgcg acatcggcgt
    21781 gcgccgtgcc gagcccggtt gcaacgttct tcgcatgcac ccagaggaac ggcggatatg
    21841 cgggttcggt ggatatgatg ccgtgggcac cagcggatga gatcccgccg acgtagacgc
    21901 tcttctggtc ggccgcgggt atgtcgccgg tccggcgctc aaggtcggcc atcgtcgcat
    21961 cgatgtaggc gatcacctct tcggcccggt cctgtttccc gaggacctcg cccatcacgc
    22021 ggaggccgcc gtacatctcg gccttctcgg cgtcgtccct gagcgagccg taggggaagg
    22081 ctacgaccgg gatacccgtc ttatcctcga gtttgtcggc ttctgcagcg ctcgttccgt
    22141 atgccgtccc ggtcgaaccc gtcttgaaga cgacatcggg gcctatgctg atgatcttct
    22201 cggggtcgtc cttacccctg aactcgccga tcagcgggag gtctttgaac ttcgaaccgt
    22261 aggcaagggc gtaagggcgg ccttctgcct cctgctcctt cttctcgatg tcatcgacac
    22321 cgacgacgag gtcctgggcc tgcaggtaga caaggtagcg caggcatcct gagcccgagc
    22381 agacgacgct ctcggggggc gacggggcgg tgacactccg gccgaaccca tcggtgaccg
    22441 ttatctcctc gccggcagca cccccgccgg cggttgtgcc ggtgcatccg gcagcggcca
    22501 tgagcagtac gagagccgcg agcgccgcgg ccgtgagcaa tctggtatac ctgaagaaca
    22561 tcatactgtt tactatgaat gtaacactaa ttaatatacc tgacgaaagg taacctatcc
    22621 agtgttaagg gatttcgttt cgtagtacat atgcagacgt caaagaacat tcctcagaac
    22681 accatcgtcg acgcccccgt ttgggatcgt cggccattat ggtcttttca ccttcctccg
    22741 ggaggagaaa ccgggctcgc tccgccggcc ggataactat tatgccgggc agccggaata
    22801 gttgatagaa ggagaacgcc ggtcgtgcaa ccccggcatg accgtccttg cagcgaggaa
    22861 gagagatgcc agaacgaatg ttaaccgaac cggaagggta tggcctgctt tcggcgtacg
    22921 ggatacccgt gccgccccac caggtggtca ccagcgccgg cgatgcacgg gcggccgccg
    22981 ggcggatagg ctacccggtc gtcatgaaga tagtctcgtc ggatatcatc cacaagagcg
    23041 atgtcggcgg cgtcgtcacc gcgatcgaga gccccgaagg ggtagaggag gcgttccgga
    23101 cgatcatgca gaacggcgcg gcccgtgccc cggaagcggc gatcaccggt atcatcgtgg
    23161 agaagcaggt gcccggcggg cttgaggtgc tgatcggggg gaagaccgat ccctcgtttg
    23221 gaaaggtcgt cacgttcgga cttggcggga agctcgtgga actcctcgag gatatctcca
    23281 tccgtgttct gcccgtcacc gacgacgata tccgcacgat ggtccgggag atcgagggct
    23341 accgtctcat caggggatac cggggggagc cccaaaagga cgaggaggcg ctcgtccgga
    23401 tcatcgcgac gatggcccgg cagtttgccg agaacccgca gatccgggag ttcgacctca
    23461 acccggtcat cctctacgag gagggagcga gcgtcgtcga tgcgcggatc atcgtcgggg
    23521 ataccgtcgg aggcgaagca gcccgcctca gcgtcggatc accccccgat atcttctatc
    23581 cccgatccat cgccgtgatc ggcgcatccg caagcccgaa caaggtgggc tattccgtcc
    23641 tcagaaacct gctttctttc ccgggaaatc tctatccggt caacccgtcc cggaaagagc
    23701 tcttcgggcg gacggcctac ccctccgttc tggatatccc cggaccggtc gattgggcgg
    23761 tcatcgccgt gccggccccg cttgtccccg gggtgatgga ggagtgcggg aagaaaggcg
    23821 tccggctcgc catcatcgtc accgccgggt tccgcgagat cggcggcgcc ggcgtggccc
    23881 tcgaggaaga ggtcgtcgag attgcacgcc ggcattccgt ccggatcatc ggcccgaact
    23941 gcctcggcgt catgatgcct caccagggga tcaacgccac gttcgacccg gtctcgccga
    24001 gatccgggga cgtggccttc atctcccaga gcggcgcgat catcaccacc gtcgtcgact
    24061 ggagcctccc ggaggagttc gggttctcaa gcgtcatcag cgtcggcaac caggcggacc
    24121 tcgggttcga acactacctc cggttcgccg agcaggaccc gatgacccgg tcgatcaccc
    24181 tctacgtcga ggagatccag gacgggcgca gcttcgcgca gatcgtccgc gaggtcgcca
    24241 ggaggaaacc ggtggtggcg gtgaagtccg gatcgtcgcg gaaaggcagg gctgcggcct
    24301 catcccacac cggttctctt gccgggagtt acgatgtcta cgtcgcggcg ttcaggcagg
    24361 cgggggtgat cccggcccgg accctccgcg acgccttcaa tctcgccgag ctcctggcaa
    24421 gcgagggcta cccgcggggg aaccgggcga ttgccgtcac cagcgcgggc gggtttgccg
    24481 ttcttgcctc cgactacgcc gaggcgcacg gtgtcgatat ggtcgacctc cccgacgagg
    24541 tgctccgcga actcaacgcg ttccttcccc cgttctggaa ccatgcaaac ccgatggata
    24601 tcatcgggga tgccgacgcc gcccggttcg ccgccctctt cgatgtgctg atccggcacc
    24661 aggacttctg ggacatcgcg ttcgtcatcg ccgtcccgac gacgctcgcc gacccggcgc
    24721 acgtcgcaaa cgagatcgtc cggttctccc ggaatacgga gaagatggtg gtgggttgca
    24781 tgcttggcgg cgacagcatc aggagcgggc tgcgcatcct caggagttgc cggatcccga
    24841 acttcgcaga gttagaggat gcgttcaaag ccgtgggaac catcctctcg gtcaggaccg
    24901 cacggtcggg cgatctgccc cgccttccca aacccgactg gtgcgcgggc ggcgagcggt
    24961 agcgcccgca ctcccgttaa ccttttatac acccctcccg catcccggca tatcacgacg
    25021 ggttctgcca cccgggagat gcatacggat gctgtttaga aagataaagt ccgatatcct
    25081 tgcgcataac tcatacatga tcggagcgga cggcgaggcg gcggtcatcg atccgaggcg
    25141 tgactgccag gtctatacga gcgtcgccgg gcgccgggat atgaagatcg cggcgatctt
    25201 tgagacgcac aagaacgagg attacgtcag cggctcccgg gagctcgctc gtccgaccgg
    25261 agcggagatc ctgcacgggg cgcgggagga gttcgggttc ggcacccctg tgcgggaagg
    25321 ggagacgttt gagttcggat cgctcgagat tgaggtcctg gagacgtcgg gccacaccgt
    25381 cgagagcata tcgctcctgc tgcgtgaccg ctcggtctcc gacgatcccc tgatggtctt
    25441 taccggcgat gcgctctttg cgggcgacgt cgggagaacc gatctcgccg gcgaggaccg
    25501 ccggcgcgag atgtcggaga tgctgtacga gagcctgcac gagaaactcc tcccgcttcc
    25561 cgacggggtg atcgtctgcc ccgcccacgg tgccggctcg gtctgcggcg ggaacatcag
    25621 cgaccgcgag tacacatcca tcggttttga gcgggcgacg aaccgtctcc tctccatggg
    25681 gaaggaggag ttcgtcgagt acaaagtgaa cgagcgcctc tacgagccgc cgtattttac
    25741 gatgatgcga agactcaaca aggccggccc gcccctgata tacaacctgc cgtacctgcg
    25801 gccgtatacg gcccgcgagc tccgttctct ccttgatcgg aaggcgcagg tcgtcgacat
    25861 acggtcgccg acgagttttg ccggcggaca tgtcccgggc accatcaaca tctggcgcaa
    25921 cggcctccct ctctacgtgg gctactactt aaactacgaa gacccgatcg tcctcgtcga
    25981 cgactttaac ctcgggatgg gccacgtact gcgccatttc gtgcggcttg gctacgacaa
    26041 catcggaggt taccttgccg gcggatttcc cgcctggttc aaacagggcg agcggatcgg
    26101 gcggtccggg atctggtcgc cctccgacct cgcggagcgc cttaacgacc cgtcgatcta
    26161 catcctcgac gtgcgcaaga tcacccaccg ggaggagtcg ggccacattc gaggctccca
    26221 ccacatctac gtcggcaatc tcgaagagcg gattaaggag attccggagg acaaagagat
    26281 cgtcgttcac tgcgacgccg ggttcaaggg gagccttgcg gcaagcatcc tcaagaagca
    26341 cgggttccgg cacgtggcaa acctgctcgg gggcaccatc gggtgggaag cggcgaacta
    26401 cccgctggtc cgggattgat cccccgggtg gttgccggga gcgaggagga gggtcagcgc
    26461 accgacgagg catgtgaggc gaagggcacc tgtacggctc tgtttccggc ccggagcacg
    26521 ggaattgcgg ttgggtctgt caagatacag taccgtcgat agacgggcga tctacccggg
    26581 tgtcggagtg gttctccgcc ggcaccgcac cgcaaggtac cagagcacga ttgtcgcgcc
    26641 gaggagcacc tcgcccgcgc agaggaggaa gggatcccgg acgacgtttc ctccctgcac
    26701 ggcaaaaccg cggagaaacg aggccacgag aagtgccccg gccgtcacgg tagcaaggga
    26761 cgtaccgaag agaaagatga tccggtggtc ctttccggga tgaagaaccc gccgccctct
    26821 cttcgtcagg gtgtagtccc gccatttccg ggcattcgcg tgcgtcacca ggtccgcgtc
    26881 ggtgaggatg gcgaggtgct cgtgaacggt cgactttgca agaccgagtc cttcggcgag
    26941 ctcactcatc gtcatcgtcc gggtatcgag caatttaaga atggtgatcc gggcgtcgga
    27001 ggagagagcg gcgatgtcat cccggtcaag cgtaacttct tccatgcttt ggaacccctc
    27061 ccttcgcggg cctggcccta accgatcagg tgttctcctg ccgaaagaga aaagcgtttc
    27121 ggaacggttc cgggtctcga tacggccgaa aaaagagttt gccattcccc ctcaccccgc
    27181 ccggagggtc gggaaggtcg ggggtgggat cggttccgat ctgtcgatcc ccatgatgat
    27241 aaacgtcgcc gaccccctgg gaaggcgagc tctattgtag tcgggatccg gagtagagcc
    27301 ggggtcgatc tcttctttcc aggaggtgtt ggagcccagc agggtgacgg tctctcgccg
    27361 gaagtcgaag tcgaccggcg tgccgttcgg ggtcgtgagg gtgtacgggg cggtaagatc
    27421 gatcgccagg tagaggacct cttcctggcc gggtctcggg aggtccgccg tcacccgcac
    27481 ctcgcgccac tctccggcgg cgaggtcgtc gataggcagc gtggtcgcgg cgccgggggg
    27541 gatgttcccc tccggggatc cggtggtggt gttcagggtt gtggtccccg tcgtcatggc
    27601 aaggtcggcc cgggcacagt cctcgcccgc aaggttccgg atgagaaagt cgaggctgac
    27661 ctgaccgccc tcgattgcga cgtcaggggt gtaccggata tcttccgggt gggccgggtc
    27721 gagactgtag cggtcgccga ggttaattgt ccggttgtag gcagtgacgt tccgcaggtg
    27781 ccaggtctcg ttcgctccgt cataggcgat caccgggctc tcgtaagggc cgcagtactc
    27841 gaaccccggc ccgaacggca gtatccagtc cacccgcttg acgggttcct gcacatgctg
    27901 ctgtcccggg gggaaagcgg cgagtatggc gaggttggcc gcggcaagga ggagaacaac
    27961 accgataccg agccatatcc acgtttttgg gtgcattgta tcacgccgga gggttcagaa
    28021 ccatatctga atcggtatgc ggtcgtataa aacccactgt ggttgttcgg ttctaaccgg
    28081 ataaaccttt agcgttacga tttacgtgcg gcagcagaaa gatgagagtt tcccggattg
    28141 tcgaccggat acaaaggttt atggtgtgtt cggctggagt cgaacggtgg gtgccggatt
    28201 tatatgatct gaaggctcag aatcgtgttg ggtgccgtgt gtggagccgg gcacacgcga
    28261 tccacccggg ggggtgaata cccggaggag agcagatgga cgaaagttcg gtcattcttg
    28321 aggaagatca gtgactccgt tgcaccacga gaagagcgcc cggttgaggc gatacctcat
    28381 cgcggtcgcg gccgccgggt tcgtcctgct cggttgctgc gtcgtcgttt cctcctacat
    28441 cgccgcgacc attccacccg acgcactcct gctctacgag gtcacggagg agacaactca
    28501 gaagggtatc gtcattcgcc tcgatgagga ggagttccgg aatttgccgg ttctggatgc
    28561 gttcctccgc gatgaggagc ataacaccga gggagagccg tatgctcccg gggacatccg
    28621 gttggtgggg agcgtagatt acccggagaa gatccgccag gaaacgatcg acgcctacat
    28681 cttcgatagc gagaccggca ggcggaagta cttcgaatac gagggacggt actaccgggt
    28741 cggaacgatt taccgggact gaacggttgg aggagaaacg gggggtgctt agaagcccta
    28801 ccgggtaatt gcggtttcca gcgcgttaag tccgcttccc gacccgaaac caattcagaa
    28861 gccccaacgt gtagatgcgc gtcacaaaaa ggtttataga agaacgttga cctctcccgc
    28921 atggataccg ggatcgatat cccatgggcg caccggccgg tgaaagatat cgatcgtgaa
    28981 tgaggcgaag agatgatgaa agaaaccaaa ctcctgccaa tactcgttgt actcctgcta
    29041 gcggcatcgg tctgcccggg atttgcttct gcaagtccgg ttgacgaaga atacaccatg
    29101 gccgccgagc gtgtggaact tactgatgat gagtacattg cggccattga agatcttaaa
    29161 tcgagagaaa acgtcgttga gacacggggc tatttgccgg agttcaaaga tgctcaacat
    29221 cgccgttcat ggtataactt cctggacaac ctcacgggtg ggatgtttga ttcggtaaaa
    29281 ccctatcttt acccggatgg ccctatgatc aattttggat acgatatcga agggtatgtc
    29341 agaatcggta tcgtggaggg ataccctcag gataaagccg gcgttgcggt ggacgagata
    29401 tacgcgatgc tgaatgagga ggcagaacaa ctggggatag aggatatccc cgtcaaggtc
    29461 gaaatagtcg accctgacag cctggaactc gatgtgcggg tcgatccgac gggcggcgcg
    29521 gcagtgtcgg ccgcgctcgc ggaacctgca gatacgacgg catatctggg acctaatgat
    29581 gcattcagcc tcttctataa ccccgtgctt ctcatgtgcg gtcttggggc cgtgatcgtg
    29641 ctcctggtgg ggtacttcct cgtccggagg tcacgcggga aggatgcacc gtgagcgtgc
    29701 cggtcctccc ggccatcgct ttcagccttg cgtggcccct tgttcttata gccctggtcg
    29761 ggaggctgga gaacgaaaaa cggcgccccg tggcctcacc cgggactgga accattgcaa
    29821 ttgcatccgg tacgatcgat acgatctatg ccggcgctcc ggcgagcccg ctggcatccg
    29881 ggatgcgtcc cgggtgaggc cccgacccct tctttatggt ggaagatccg gatgaaatct
    29941 ctgaaaaccg acagaaccag caaaatcgca ttcattctcc tcctgatagc cgtcggtgct
    30001 ttcggcatcc tcctgctcgt tgatctcctc gccattggtc ccgggtatcc tccccctaaa
    30061 gccctgcaga aatggtatat cccgcagccc cggtacgaat atgccgagaa cggctctgtc
    30121 gccgcaaata gaacgattga tgggaatgtt gtcctgctcg gcgatatcga agacgggtgt
    30181 ccatccttgt ttccggttct ctcacggcgc tgcagccatg caatgtatgt cgacaccatg
    30241 tcgggcgatc ggtatctggt cgtcaactgg tatttcgacg ataagacaga cctcttacag
    30301 gctgagacgg tcctctgcat ccacctccga gcatcgggga atgtctcttc tgctgagatg
    30361 ttcctctcca gagaacctga attatcacgg ggagaaccgg tttttagtcc cgttgccgta
    30421 acaaaattcg ataacaagac gtcttcaggt tatctcgccg ttgttgagaa gccgctgtcc
    30481 ccaaaacacg acgattactt cattgtctac tatggggtgg tcgggccggc cattctccct
    30541 gaccacactg ctaccctgga gaaccttatg ctcaggagtt attcgtttca tggtgcaagg
    30601 ccgctcgccc cctgctcggc acgggaagcg gcaacagggt ctatgcattc tgtgtgcgaa
    30661 ggcatgcccc tcccgcctcg ggctgctgaa tctaccgcgg catccaatca gaggttttga
    30721 catgcgatta aaaattgttg cactcgtctg tctcgctctc gcgatcatag ccgggtgcgt
    30781 atgggttctt ggtccgggcc aggctccgga aatccctctt cctgccagag cgggtgcagg
    30841 tgaaactatg ttctccctca ctgccgagtt cccggagatg cgggactcgt acacgctcta
    30901 ccggacagtc cccgtcaccg tcacggaaga gcaggtccgg gaggttgcgg agcggttcgg
    30961 gcttgcaggg gaacccggga cgatgaacaa aaagaccggg gagttcctcc tcatcgatgc
    31021 ctcgaaggac ccggaggagc aggtatcggt gtatgcacat tccggcgccg tggcctatca
    31081 catccccgac ctggaactgc cgaccgaagc aatccggcag ccggacctcc cgacggacgc
    31141 ggaggcagag aggattgcgc ttgcgttcct tgagaagacc ggcatgcagt cgccgggtgc
    31201 ccggatcgtg aagattgggg tgggccagaa gcaggaggtc tggaaggccg gcgagagcga
    31261 gccggaggtg tcctgcgatg tgacgaaaac ggttcggttc ggccggccgc tcgacgggct
    31321 gccggtctac ggcgacgaat ttacggtgat cctgggggac ggcggcaagg tcgccgggct
    31381 cgtgaagacc tggcgtgagg tgacgccgga cgggaatatc agcctccggt cccccgagga
    31441 ggcatatgag gatctccttg cagcaaggac catccgcccc cgtgtgggag gggagtacgg
    31501 tcggatcacg atcgagaatg tctcgatcgg ttactggatg gaaccgaggg gtgtcgtaca
    31561 ggatctggtc cggccggtgt atgcgttttc cggaacagcg ttccgagacg gcgttcctga
    31621 accgtatatc gggtatgtct ttgctgatgc aggggggtga tttgggatat gatgaacggc
    31681 atcaaagtgc ggcttttcgg cctgctgctg gcatcactgc tcatcggggc gttcttcgtc
    31741 ccggtgatct ctgccggcgg ggagagcaca aactggcttg atacctttga cgaacgggtc
    31801 acaccccggg gcatgtcgac gtacaacaag ccgatagtta caaaagaacc ggagcagccg
    31861 ggcccggaac cggccgagat acatcccggc ctggttctgc tcggggagaa cgcgacgttc
    31921 cccgaacgtg agtctatgac gatatcgggc ctgccgtccg gccccacaac gcacgttgcc
    31981 gtggccgatc ccgcctactt cagcccggtc ccatcggccg gcgaggtgaa cctcacgaac
    32041 ctcccgcccc tccgaaagta tgacctcgtg accgccgacc cggcggcgtt cgtcgctggc
    32101 gcgggctcgg ggagcccggt gacgctccgc ctccccgacc gggagttcgt actcgacctc
    32161 gaaccggtgc cgggccccgt cgccgaaggc gcccgggcct ttgtcaagaa tgagtcgggc
    32221 acgtttgaag tcgctcttcc tcatatatgg cgctttgagg ggacggtcgt gggggagccg
    32281 gggagttccg cctcgtttac ggtcggcggc gacgcgatcc tcgggacgat ccggtgcggc
    32341 tcgacattgc tcgtcatcag ccaggccggg accatcgaga tcgacggaga gcagagggtt
    32401 gtgcacgtca tctatgacga gcgggacatc accccgagac tccggtcccc catcttcgat
    32461 tgctgcgtgc cgccggccgg ggccagggac tctccggata ttaccgaaaa tcggattaca
    32521 agccttgtaa agagcctgat agacgctata gcgacgggaa gaacctgatg accggagggc
    32581 tgccggtaca ggggtatcgg ccgactcccg ggtagccggt tcaccggaga agaggagggc
    32641 atcattccac tccctctggc tgctccggtg ggagtggaac gatgcctcct tctgcatgac
    32701 tgggtggctc cctgcaggtg agataacgaa ggttgccgca tcgatcggca agtgatgccc
    32761 ggagcgacca tgagccagct tgccctctca ctcctctttt cgtagcgcct ccctcagggt
    32821 ctcgatggtc ttctcctcat tcattcccgt cgacgatgcc tccacacttc accaggaatc
    32881 gcggagccgc ccagataagt tcctcgcaga agggatccgg aacttcccgt gagacatagt
    32941 accatgcatc gacccgtgct cctcttgctt tttccacgag tgccgtctcc gcaggagtga
    33001 aataatctga caaaagccgt tccatggaca aaatggtgca ggaatggttc tcgcaccgta
    33061 tcccgatccg cgtgagaacc gcatcgaggg agaagtacgt cgcgtaatac gcggtcgtta
    33121 tcattcagtc gcgggccgta atcgtctgca tcgtctcaag ggcctcttcg gccttcttca
    33181 ggtatgccga tgccagggtc tcgttcggtg cggagaggga gatgccgttt ttgatccggt
    33241 tgcaccatac gagccgatcc gtcacctcac cgccttctcg aagaagtgtt cggcgccttt
    33301 gatcagcacg tggttccgga gcacttcccg gagaaagggg tccgtatgga gatcccggtc
    33361 gaagatctcc cgcgggtaga tcgtgaggtg aatcggaaag ccgtaggtct ttccgatcct
    33421 ctcaatttcc tccacccggc actcaccggc aacgaagagg tcgaggccgg actcctcgtc
    33481 cgcagtccct tctgcgtgac tcccgaagac cgcaccgcat ccctcgataa gggggcgatt
    33541 cggctgagaa ctccggcgag gatgggctcc tcctcccgga agcagatccc caggtagatc
    33601 tctgcaaggg tgtagaagtc ccgcgcatga tcgccccgcc gcagggagaa caaccgcgcc
    33661 ctgcccccgg tggcggagcg gagaacctgc tttgcctcaa ggtattcaag gaccgcctga
    33721 gctgttccgc ggctgatggg aaggagcctc gcggcctccc cgatgtaaag tttgcgatcg
    33781 tagccccggg tgaagaggga gagaaccatg aggtggttct cccgaatgtc cgatatttta
    33841 gacatatgtc cggaatattg gacagtcctg tatcagggtt ccggttgccg ggtggttacc
    33901 ggagtgaagg ctcccctgcg cggctccggt ttccggccca ctgcggcggt tttcggcccg
    33961 aaatcggtca cgaagacacg acgtgcagac gcgcggcaca gaaaaggttt ataggctagc
    34021 gtcgacgtct ccaccatgag caccgggacc ggtgtcccgg ggcgcaccgg
//
LOCUS       DAOSEE010000005        31680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_693_length_31680_cov_269.907510, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000005 DAOSEE010000000
VERSION     DAOSEE010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 31680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31680
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_693_length_31680_cov_269.907510"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2598)
                     /locus_tag="PLI76_00910"
     CDS             complement(<1..2598)
                     /locus_tag="PLI76_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60618.1"
                     /translation="MKRLGGLLLLITLLIPATTATTIGISPDAIEPGGTITVNVDDLP
                     DGTSFALGIRSEFTTIPGEASVFTVQNLTLPFALNSGEMSAYTRGTNWTELAVNLPDG
                     GTVIILKYADQNGEVRIVQSCNIESGTISPVTLRGEAAASSITADLTMLGTKQGQDSG
                     IILFAIEGIEQDIVTVTVYVDGCEALSRVIPVGASPTPTPPPTQAPPVSRAPAIVSNK
                     DSIIRGNCFVVTVTGAAKKDYRLYVREISGVAPGAYPVIVPGQIAVPSYTVPGEDDSN
                     RTIMTNATGTATIQFNTSQSTGEWRYTIRVEDPADPTIYDDVRVRVEHGAVTITTSGT
                     GVYYLGEEIIISGTNTDGDTTHLFLTGPNLNSNGVRLDNISSVANNNAASFTKANVEA
                     DDTWVYRWDTSSLSRMLDAGGYAIYAVAEPRGKNALSGTQYATTYIQLRSPFVTATAS
                     AAAITRGDELTISGRATGAPDNVYVWIFGKNFYGNGNQLYARPICVEVDGSFEEKLKS
                     SDTENLAAGQYFVIVQHPMGDSPGVVADPATGMISGPGINPIYLTGLQAAAAASALIN
                     VLDSPNVPDTYTKFSFRIIEEGWIQIDPISDPTYGKTFTVTGTTDYWKGTALTYEIIE
                     DYEGFALSGETVVTGDGSWSIEVDTAAIGPGAFTLRVTAPNGLASATAVFDIYDDVTH
                     PAPPGGAAYRVERISTTPALDDLASGEEVTLYGTLSSTFWAEYQGDLAFLTDLENPTW
                     SGSVSRDGVVVYTQSGGAKSLTLTAFELGYGRGKIQVFLTLSGTVPEAGTNDPILLGI
                     LQRDADGRVIPNSEYRLPLTPAGDSPPPPSPDNLTLFPGWNFISIPRPLAAENDTAAI
                     FAGVETGGRS"
     gene            complement(3486..4577)
                     /locus_tag="PLI76_00915"
     CDS             complement(3486..4577)
                     /locus_tag="PLI76_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60619.1"
                     /translation="MKANISRWIILLLALVCVVQAVSASFDIKKEDINPASGALEPGQ
                     QVTAHYVLSYSMITPDDASDETFEFSTGLQSPTWDFIVYRDGVDIYRTTKTGYYPTLT
                     EFEISYGKGKIELDIQLRGTVPESTTGKVEVIKIQHMRDKDVKDTYSKVRDVVSTQQV
                     TGSLSVQQQRLKDLKADIDDKAGKGVDVAATQAKYDAANQDLTHAASAGPSQAAGYIA
                     TATKAIDEAKALLDKAWAEKEVSNAAATLETLDGMITYFVENRSMGSDPQVVAIMTKR
                     ESAVQFYSQAKDNLNANNYPLARSKATEGQNKANEALTDANTLHEKIGDGFNLDSNLL
                     LYIGAGVIIVLVIVGIVIYRKKTGWDELG"
     gene            complement(4587..5753)
                     /locus_tag="PLI76_00920"
     CDS             complement(4587..5753)
                     /locus_tag="PLI76_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tubulin/FtsZ family protein"
                     /protein_id="HOI60620.1"
                     /translation="MRVFFIGFGQAGGKVVDMFIEQDKRMQTQSFRGIAVNTARTDLM
                     GLKHIELKDRLLIGQTVVKGHGVGTDNVTGARVTADEIDSIINAIDSRGTHDVDAFVI
                     IAGLGGGTGSGGSPVLARHLKRIYREPVYAIGILPAPEEGRLYSYNAARSLSTLVNEA
                     DNTFIFDNSAWKNEGESVKDAYNRLNDEIVRRFGVLFRAGEVGKAGVGEMVVDSSEVI
                     NTLRGGGISTVGYAISEKVNPRAKQSKGLLSGLMRKKEKTEEVLTGEDKSAKIIALVR
                     RAMLGRLTLPCDYSTAERGLVLLAGPPDEMDRKGVEKSKSWVEENIAGVEVRGGDYPV
                     QSDYIAAVVVLATIGNAPRITELLEIAKATKEDVLKSKEKRTTMFDDGIEPLFE"
     gene            5868..7100
                     /locus_tag="PLI76_00925"
     CDS             5868..7100
                     /locus_tag="PLI76_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOI60621.1"
                     /translation="MKVCIVGGGLCGLVSALELAGAHQVDLFERRPVPGGCLGSYRIG
                     DYWIEEYYHHCFSGDTSLLNLFDTLQVADRLVWLKGTTGYYVDGTIHPLTSPAEILRY
                     PHLTLIEKARLGLLTLRSRQLDAPALDGITAKEFILDNLGPGIYASFFEPLLKSKFGD
                     RREEVSAAWLISRIAIRSDRGAGGERLGYLRGGFRHFVARLEEEAANRGASIMPDSPV
                     REIRREGDGWLVDGEVYDCVVSTLPPQVTADLAGLDIAPVPYQGAACMTLALDRDVTD
                     GIYWLNMKDAAPYGAVVSHTNFAPFEWYGEHIVYLASYFSGRLPEGLEQSMLADFCRR
                     FFVSPDEVHWHRLAVDPHAGPVYTTGLRSRLPAYEEHGLFLAGMFSPPNYPERSMNGS
                     VVAGKEAAKRVLARYPDV"
     gene            7093..7812
                     /locus_tag="PLI76_00930"
     CDS             7093..7812
                     /locus_tag="PLI76_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOI60622.1"
                     /translation="MCEVEVSAVLPVYNDLSALKVAIPRSLETLEAIAPGGFEVIVAE
                     DGSTDGSAEFVGAYEADDPRVRLLHADERLGRGRALNRAFAAAKGSIVCYYDVDLATD
                     MQHLPELVGAIRDGCDVATGSRLLAESTITRSGGREIASRGYNMLVRTILGSRLHDHQ
                     CGFKAFRRDRLLPLLPSVSAGHWFWDTEVLVRAQKNGYRIREFPVRWQQGEGTTVRKK
                     DVAEMGSAVVRLWWQLHVEEG"
     gene            7796..8767
                     /locus_tag="PLI76_00935"
     CDS             7796..8767
                     /locus_tag="PLI76_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOI60623.1"
                     /translation="MWKKVSTIVIPTLVAVGILAYMLYSVWDNLLLTLEHAVIPFLFA
                     AIGICVIAWILRGFRYQFILEGLGVPESLRFATACIFISQTANLIVPARLGDFVRMLI
                     LRHEDNATYSQGFSSIVVERVFDILMVAVLGAIALPFVLAVPDWFITVIVVPIAAGGV
                     FFIVLLWSGRMKSENRFVVAVQRMLDEVKQASLNPRALLMLSSSSLLIWLVDVLVCAA
                     VVLMFQEPVPFAIVVLAIVIGNLVKAVPITPGGVGTYELALALTFGLAGTPAATATLI
                     AVIDHLIKNMVTLVGGIGSIYYFGDWSMDLLKKVFSREIDKEETFGG"
     gene            8757..10757
                     /locus_tag="PLI76_00940"
     CDS             8757..10757
                     /locus_tag="PLI76_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HOI60624.1"
                     /translation="MAGEFIVPVLAWLAVIKALHLGTWPALDRTLGSLAAAAAYPASV
                     LLFTLLSWYCGILGLPILLALLPFLAGIALAGSRGFYTKERLRSTFAWDLAFLVPFLF
                     MLEVRWINPTISYAEKFMDHAFIASIMRQPIVPPLDPWYAGGALDVYYYIGYWMNGML
                     GLASGVPSTVTFNLALPTVLGLAVVSLYALGHLLLDRHRWLPVLALLIPNPSAIYHIV
                     IGNSWFDVLWGSTRTIKDTINEYPIFSMLWGDVHPHVISIFNQGFLLFLLVFAYMRWG
                     TLTMRGRVILCGLAALSLGSMPGINSWDVLIYAPVTLIFGLLVWRRYGGSALDDERSW
                     MMLAAVPPLAVAAYLPFYLQLGSSGIGGIGIVPAPSDPVEFLLVHGFFLAILVASSAP
                     DIRRRPYLLAAAVPFLLAGYPAAAIAVVPLAYLIARRRFTAADTLAILGLGIILATEI
                     IYLKDNMGETYFRMNTIFKFYLPAWLLMGTASLAIVGEWLGNAGIDGRMPARVERVLP
                     ALAVVALLAMPFAINIDFGYGSHTLDGAAYLEGSHPGDAAAIAFLRNLPGDQIIVEAE
                     GGDYTYYSRVSSFSGIPTIVGMPFHEYMWRGDAGRITERSVDVRAIYEQPSRAVDLAR
                     AYNATLLYVGDEERDRYRVSIPADVLELIYDAEGVQIYRIPG"
     gene            10937..11305
                     /gene="rpl7ae"
                     /locus_tag="PLI76_00945"
     CDS             10937..11305
                     /gene="rpl7ae"
                     /locus_tag="PLI76_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7Ae"
                     /protein_id="HOI60625.1"
                     /translation="MAKAYVKFEIPAEIQNKALEALEIARDTGKVKKGANEATKAIER
                     NIAQLVLIGSDVEPEEIVMHLPPLCEEKQIPFVYITKQNDIGSASGLEVGSAAAAIVK
                     PGKAKDLVDEIAKQITALRA"
     gene            11316..11525
                     /locus_tag="PLI76_00950"
     CDS             11316..11525
                     /locus_tag="PLI76_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S28e"
                     /protein_id="HOI60626.1"
                     /translation="MANDGTPAEVIEIIGMTGMHGEAQQVKCRVLDGPNKGRIITRNT
                     VGPIREGDILSLLETEREAKKLSRR"
     gene            11531..11722
                     /locus_tag="PLI76_00955"
     CDS             11531..11722
                     /locus_tag="PLI76_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24e"
                     /protein_id="HOI60627.1"
                     /translation="MVDRYICNFCGEPLEPGTGKMFVRRDGTVYYFCSTKCQNNYRLG
                     RVPRRVEWTAAGRKARGKE"
     gene            11729..12178
                     /gene="ndk"
                     /locus_tag="PLI76_00960"
     CDS             11729..12178
                     /gene="ndk"
                     /locus_tag="PLI76_00960"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOI60628.1"
                     /translation="MDRTFVMVKPDGVQRGLVGEIVSRFEAKGLKLAGAKLELLPEGR
                     VVEQYREHLEKPFFPGLKAYIMSGPCFLMAWEGRGAVAVVRRMVGATNPAEAAPGSIR
                     GDFALDIGRNVIHASDSPESAARELGIHFSPRELVEYSRIDEPVLYE"
     gene            12378..13031
                     /locus_tag="PLI76_00965"
     CDS             12378..13031
                     /locus_tag="PLI76_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter"
                     /protein_id="HOI60629.1"
                     /translation="MADLLSFALLSFSSIVIVVNPLAATLIFVSLTGTMDGATKLKVA
                     RDASRFALVVLLIFTVAGGWILQLFGISIEAFRIAGGLLLFGIGMDMVYAKTSRTKMT
                     ATEKYEGQDADDIAVMPLAIPMITGPGAITSAIVLMNEAMSMGIAALAVVPIAAGAAI
                     AITYYMMQHSDIIVRRIGQREYRAVNRLMGMLLIAIAIQFILIGIRAAFPILTGGPA"
     gene            13028..13231
                     /locus_tag="PLI76_00970"
     CDS             13028..13231
                     /locus_tag="PLI76_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60630.1"
                     /translation="MTPDLTFPGMVLISSSIIVGGILIGALTFAIDVPYASCTMVVLA
                     ALIVLGATMLVLSTRNQGGVPSR"
     gene            13231..14010
                     /locus_tag="PLI76_00975"
     CDS             13231..14010
                     /locus_tag="PLI76_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOI60631.1"
                     /translation="MTKIALAQVLGGAARALDTAGRMAGEAALAGASLICFPEQFVAG
                     WLPEIPPGSGEPLDGPLATAFGRMARESGIAVAGSIIEARGGGRPQNTVVILDANGEL
                     LATYAKIHLFSPGGEDRNYTPGDRVATFTIDGMTFGLAICYDLRFPEIFRIYALAGAE
                     CVLLPAAWPCRRLRHWETLIPARALENQYYVAGVNTVGGVYCGGSLAAGPDGDTIARG
                     GSGEELLFVDLDPAAIHEARMRLPTFSDRRSDLYHRLLSNL"
     gene            14020..14763
                     /locus_tag="PLI76_00980"
     CDS             14020..14763
                     /locus_tag="PLI76_00980"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOI60632.1"
                     /translation="MRGDQHVYLSLTTAGLLIAPFVPVLDPALIVTLVFGVFVGSLAP
                     DADAVDAAIFNGWIGGAKGKRGQVLNGVAVVLPVFGYTIRYLIYYPLSLVFSLILRKS
                     YRHRHRGLLHSLAGVGLTSVILSVYLAFILTWIGWSPALLPAFGVAFFAGCILHLTED
                     ACTPAGVAWLYPFSRRRMAGRIRPWGTFEIRPAAFAAVLALAAAGVFIAPFVTAVSPG
                     ALRCLALAGTLVLWLLFMLVCRVRCERRR"
     gene            14822..16027
                     /gene="thrC"
                     /locus_tag="PLI76_00985"
     CDS             14822..16027
                     /gene="thrC"
                     /locus_tag="PLI76_00985"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOI60633.1"
                     /translation="MYRLVCVHCGATYTPDQIIYTCNRCGHLLAVEYDLDGIDVSRDE
                     WNRRPLSVWRYRELLPVGGEPISLQEGGTPLYHLKNIGKELGLPELYAKHEGMNPTGS
                     FKDRGMTVGVSMARELGMTSVACASTGNTSASLAAYAAKAGIPCVVLLPAGKVAVGKV
                     AQALMHGARVISIRGNFDRALEMVHELCISQGLYLLNSVNPYRLEGQKTIGFEAIDQL
                     GGDVPDRMVLPVGNAGNISAVYKGLRELEALGFIDRLPMMTGIQAAGSQPLVKAIAEN
                     LDELIPEPAPETVATAIRIGAPVNAEKALVAVRATGGTAAAVTDEEILAMQRDLARKE
                     GIGVEPASAASVAGIRKLAEQGLIDKDERIVCVVTGHLLKDPETVVRQCVPPLEIDAD
                     LHSLLSALR"
     gene            16048..16584
                     /locus_tag="PLI76_00990"
     CDS             16048..16584
                     /locus_tag="PLI76_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5803 family protein"
                     /protein_id="HOI60634.1"
                     /translation="MAAKEATFRVLPGGTTYEASVQVTGTGYGFWGPGLMGERVPLQV
                     EDLKVLGPAGPVDYREAGRGAIAFPEGNYTITYQVPVRDNHLVAIFDTPYAVTVALPE
                     GLDVKNPLIGMVSPGGAVSPGPNETMEITWDRTDAVEVRFYAPEREILLTTFGTIWLS
                     VAIVLLLPYFISWRRDGG"
     gene            16581..17156
                     /locus_tag="PLI76_00995"
     CDS             16581..17156
                     /locus_tag="PLI76_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOI60635.1"
                     /translation="MIDIVAASFLIGLSGAASPGPMTASILGLGTRRPGPFVAGLIAG
                     HAVPEAAMVMAIAYGVRDIPYIDLIALIGSGVLIAFGVSQLLHAGDTLAASRETRAPV
                     AVGVACTLGNPYWWVWWLTFGVGFLALHPSFAAFYVGHIGADIAWLGLLTFAVSRGAN
                     VLGPHYKKVVQASGLAMVLFGLYFILTVFLP"
     gene            complement(17131..18039)
                     /locus_tag="PLI76_01000"
     CDS             complement(17131..18039)
                     /locus_tag="PLI76_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOI60636.1"
                     /translation="MAEKEQGCELFSAGHRACGGCGPALAARLLLKATGKNVIIAAST
                     GCMEVFSTPYPETAWEVPWIHSLFENVAAVASGIEASLKKQGRSEKVVCVAGDGATFD
                     IGVLCISGAFERGHDITYICYDNEAYMNTGIQRSGATPYGASTTTSPAGTCSPGNLLP
                     KKDMPAILAAHGAPYVATASVAYPADFMKKVERAINTPGPCYIQVHTPCCTGWGFESG
                     ETLAMGKLAVDTGLWVNYEIVNGKVEKVKKVRRKPVEEYLAKQKRFRHLFKPARQDDV
                     IAHIQAIADANAERFGIDIKAKTPSE"
     gene            complement(18040..19194)
                     /gene="porA"
                     /locus_tag="PLI76_01005"
     CDS             complement(18040..19194)
                     /gene="porA"
                     /locus_tag="PLI76_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HOI60637.1"
                     /translation="MLEFMEGSHAVAEIVKRCRPQVISAYPITPQTHIVEDLAQMVAN
                     CELDAEYITVESEFSALSACLGASAAGSRVYSATTSQGLALMFEVCFNVAGMRQPIVM
                     TIANRSLGAPLSIWNDQQDSISLRDSGWLQFYAEDNQETADLHMIAYKVCEDHDILLP
                     AFVCFDGFILTHTYEPVSLPTQEEVDAYLPAFTPYQRLDAANPLSMGMYATPEYYMEF
                     RYEIDRAMQRARQSFIKAGREFGEQFGRDYSGLVEGYRLEDADTALIAMGSICGTVKD
                     AIDEMRDAGKKVGLLKIRTYRPFPAPEIVDALKGVSTVAVLDKNISLGNGGAVGTEVK
                     SALYGSGVTVYDYIIALGGRDVRKRDIAAVVDLAEKGKGDMFYGLRTEVL"
     gene            complement(19194..19454)
                     /locus_tag="PLI76_01010"
     CDS             complement(19194..19454)
                     /locus_tag="PLI76_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOI60638.1"
                     /translation="MALRVGCAAPPGRARDNKTGTWRVFKPVFDPETCNRCGMCAMVC
                     PEGCVRETEEGTFEPDLDYCKGCGICEEICPKKSIRMEKEEK"
     gene            complement(19454..19999)
                     /locus_tag="PLI76_01015"
     CDS             complement(19454..19999)
                     /locus_tag="PLI76_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase subunit
                     gamma"
                     /protein_id="HOI60639.1"
                     /translation="MKELRIHGRGGQGSVTAAELIAFAAFEGGAYAQAFPAFGVERRG
                     APVQAFVRFSDAKIRLRSQIYEPDYIIVQDSTLIGDVNVFMGMKAGGIAIVNTEKPDF
                     DSRVPEGVKVYTVDATTIALEELGVPITNTTLMGAFAAATGEIGLEPLKHALQRRFSG
                     SMAEKNIRAAERAYNLIGGAA"
     gene            20243..20794
                     /locus_tag="PLI76_01020"
     CDS             20243..20794
                     /locus_tag="PLI76_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60640.1"
                     /translation="MRVSRGLVPICCILILASFCVVPAWASDAAVSREIDRSSVPVGG
                     EVLVTLAVDDLGVGGVVETIPEGFVFLETSFPEDRYSVSGQRLTFAVIDERKITYRVQ
                     AKAAGSWTFSGTWVDAVDKTEGAIPPTAVTVGSGAAAAGAAPAGTGQAPGPTSAGGAP
                     WAVVPALAAAAVAAGACAARKER"
     gene            complement(20810..21664)
                     /locus_tag="PLI76_01025"
     CDS             complement(20810..21664)
                     /locus_tag="PLI76_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI60641.1"
                     /translation="MIEVTDLETNFSTGFIHRRVVRAVDGVSFWIEEGETLGLVGESG
                     CGKTTLGRSILRLIEPDRGRIVVDGTDVTALSRSELRRFRPRMQMIFQDAYASLNPRM
                     RVSESVAEPLKIHRMGSRADIDRRVRELIEEVGLNEEHLNRRPYELSGGQNQRVVLAR
                     VLALKPRFLVADEPTSALDVSVQAQILNLIRDLGHDYRMTCLYISHDLGVTRAMSSRV
                     AVMLEGRIVEIGRTADVFDRPCHSYTQRLVDAAFAGRACTPPERPPLPAAPTMVEVRS
                     GHWVEETV"
     gene            complement(21657..22607)
                     /locus_tag="PLI76_01030"
     CDS             complement(21657..22607)
                     /locus_tag="PLI76_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI60642.1"
                     /translation="MTAMSDALLEVRGLKVGFPTPEGLVRASDTVDLEIYPRETLGLI
                     GETGSGKTVLGMAILRLLQPNTIIEGEIRYRGRDLLSLPEAEMQRVRGREIGIVLQNS
                     GTSLNPVYTVGTQIAEAVTLHQGLSGPDARQRAVELLDAVGIPDPVRMAGEYPHRFSG
                     GMRERALIATALACRPTLLIADEPTKGLDAVTKRGIVDLFSEIDRDRSVLFITHDIET
                     VEDLAARVAVMYAGEIVEVGGVREVFGDPAHPYTRGLLASLPERGMRPIPGSAPSLIT
                     LPPGCRFSPRCGCAIGGCNREHPGLVEVGPGHYARCMLYD"
     gene            complement(22604..23428)
                     /locus_tag="PLI76_01035"
     CDS             complement(22604..23428)
                     /locus_tag="PLI76_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI60643.1"
                     /translation="MPRKKVILAASFGIVFAYCAVALLAPVIAPYDPHAITGAPLSPP
                     DKDHLLGTNDLAQDVFSGLIWGTRATLLLGFIGAAIAVAIGTAAGIVSGYYGGLIDEA
                     VMRLLDVAMTIPIFPLLLILTTFFRPGIYLIAVLMGLLGSFQGVRLIRSQVLSLAVAD
                     FVVGTRALGAGDGYIMARHILPGVLPLAGVKFVTSAQHFMLIGIGLGFLGITDPSVVD
                     WGQMINRASQNGAMILGLWWWLIPPGLAVTLISLALAWLGYACEDEINPRLRVMRV"
     gene            complement(23421..24401)
                     /locus_tag="PLI76_01040"
     CDS             complement(23421..24401)
                     /locus_tag="PLI76_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI60644.1"
                     /translation="MNGIYLLKRLITSFAAFCIAVTIMFALLRLMPGDYITMYLEAIY
                     RYAPPDVVEAFNHVYGLDRPILEQYVIYLLDAFRGNWGYSFIYKVPVLTVIGERLVWT
                     LILLLPATILATLAGIGIGAYSGWRRGSKTDIALLNSMIIIRAIPSYWLALMILIIFA
                     YYLNLFPLGGYVDTGALTTGIDPLDVANHAVLPVLTLFLFSVTGTYYLMRSSMLMTAG
                     EDYITTARAKGLDELMILRKHALKNAMLPMVTLITFECAGMITGSIFVETIFSWPGLG
                     MLTYKALAARDLPLLQGILLIDTLFVIAANIAADLIYPLVDPRVQVGDDA"
     gene            complement(24460..26271)
                     /locus_tag="PLI76_01045"
     CDS             complement(24460..26271)
                     /locus_tag="PLI76_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOI60645.1"
                     /translation="MTTRCLSKILACCVLLLVCCMPATAGIPGDTDGDTNISADELAT
                     AILGYLGGTGALTAEELSLAAHNHLHQPYGQLVAAVLGETYLIPSTSYIDQKGVPADS
                     LIHEGLVTKLRPQDDGSGGYLGWLAERWEMSDDAMSWTFYLVKNATWHDGTPFTSADV
                     QFTYEYFRDHGDADAPAGSFLVNSAILSNVASVECPDPYTVVFRMKSCAPLFPETLAT
                     GPGIGVFPAHIWKDIDLPSTYVDSKQIGIGPFAYTSTIPGTLSKVTAYEGYHGEQPYV
                     REIVIRNYEDEDAAILALRRGDIDFISGEFGLSPRKAEALKGAPGIGITQIPSGGQAF
                     EVAFTSDFYPANITAFRRALSHAVNRERVCWLVGDGARPTETAFLLPALAGDQINPAT
                     NGRFSYDIEEAKRQLVAAGFNLTEANGRPVLLDPDGNRVEISIPLAGKASTNAVDQKI
                     VAVLKEDWEGKLGIRIVETPQVDDSIYLDWIARTAVHFDGMPGRWHEDIDRLDNFQRS
                     PLGENYYHFDNETFNGLITELKSTADPDKREEIGFELQEILAEEVPCIPVFSQDAFMA
                     YRSDRFVGWDAALLGGGGNIRLFSEIRPASAEVGLRA"
     gene            complement(26277..27188)
                     /locus_tag="PLI76_01050"
     CDS             complement(26277..27188)
                     /locus_tag="PLI76_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOI60646.1"
                     /translation="MIRPDQVIIVFLVLMLALLPAPALAAGAGTTELRIVKYANDRTT
                     VLNETSVDYRWLEANLPVQGDGVTRYYHQGPVFEGIWDEVHPDEPYDGWNPGEDVLMS
                     MLVKADFGAVKGTDLADICDSVGGASEGDEINLHSRDGFSKTFPYSIVYEPDSRQGPA
                     VLCWYSGNGSGSDAQEAQGQGYPDTGYTVGMRLIFFADDSTNPWGWHIFGNNDMRECW
                     GKAYWNYGGSYPSAAGASPKWVDEVRIYSKEDPAANHSAATREPVEGGETPASLSPIL
                     GSVAALLAVLGVSFRYLRHEPDDDQSR"
     gene            complement(27370..30237)
                     /locus_tag="PLI76_01055"
     CDS             complement(27370..30237)
                     /locus_tag="PLI76_01055"
                     /inference="COORDINATES: protein motif:HMM:TIGR04213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOI60647.1"
                     /translation="MPVFILVVALLVAPALAAGTTDVRIAKYAVDGTTVLNETTVDYH
                     WLKANLPVQGDGVTRYYHQGPVFEGHWEDAHPDEEYDGWNPAEDVLMSMLVKADLGAV
                     MGTDIADICELVGGAAEGDEIAVRSRDGWQSKRYPYEYIYDPDPRQGPAVLCWFSGNS
                     SGPDAQEAQGQGYPDTGYDVGMRMVFFADTTTNPWGWHIFGNNDMKECWDEEYWNKGG
                     DYWSAAGTSGKWVNEVRVYSQEEPEPPTPDFGANVTSGTVPLAVAFTDRSTGVPTPTS
                     WFWDFGDDTTSDEQNPTHTYTAVGIYTVTLTVTNVAGSDTAELSITATSGSDGGDTAD
                     TESPGSDNRTFLFEVPGIDEIVVFAVEIPQNLNLSCEEILLPDGIPEPPGAVYRYYNI
                     TSRNGTVAVDRAVIRFNVPDAWLQENWIVGDDLTLYRYNETWAPLETQWIGTENETNT
                     YEAVSPGLSLFAVSGIQIKPATAATTAPAPTPTPLATTPTGTATPAVSGTPAPAGKTP
                     ENPLVSIFGYTGVSTVGGLPGRLLGAVQAFLGLFGLLPAAADPVTEPEPVPTAPTPAL
                     VQAAPEPTPIDLTAMHFGLSVLSDPPGALIDLDGEYTGRTTPAAFASLPGGTHTVRVR
                     LDGAEPVEREIRLAHDDEVVIEIPVSASVRSASPGLLRGREQNHYGGVYVDSFPAGAE
                     IFVDGRKVNRKTPAVIYGLREGLHTIRVQEEAVAFSSGGEQVWIERNTVARMMFTGGN
                     AELTRSIAFESEEYAKQPFSVNGRYLGDRFPKTVEVSGIGEAYLAVRDGMSYRTYRIP
                     TVAGSGDTVNAMFSAAPCSLLVTSTPAGAAIAIDGFQTGFATPYLVDNVSGGKHLVSV
                     SKPGYIPEEREILLTVGSGDATAKFILEPYTYGSLSVSSTPADARIYLFGRDTGEKTP
                     HTFHYLPIGSYPVKVTEVNRTVTEDVLIAPYQIARMEVPEV"
     gene            complement(30386..>31680)
                     /locus_tag="PLI76_01060"
     CDS             complement(30386..>31680)
                     /locus_tag="PLI76_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOI60648.1"
                     /translation="WDFGDGTNSTLRNPTHVFDHDGEGARTYTVTLTVTNSGGSDVKT
                     TAITVVAPPQKEDVTDRVDQTNATATEDISVTYQGLTLEIPKGHDAKQADGSPITEFS
                     VGVAPDLEEPPAGTIEIGGKAFKLGPEGAKFNPKIPITITFTQDEWEELFGDGRTTKL
                     QRYDGQNWIELENQTRDDTTFTITGYTDSFSVFAPVTTTTTPEPTSTPTQPRTSGSGS
                     STATVTSTGTADLLTASWGGVLRPYLVYVDGKFAHLSLETGVTALDDAGSPLSGVGIA
                     PVSTLPGASSLAFSGYAVDCSPAGATFSPAIDLVFTFTGDEWAALLGKTGGDSARLVV
                     QGYDDATGTWTACPTNVDADARTVTASVSHFSTYALFVGTATEAPVPTEPTPTVPAAA
                     ATTPPVPPAETGESPLVWIALAVVIVVALAGYLLWKRR"
BASE COUNT         5573 a         9927 c         9947 g         6233 t
ORIGIN      
        1 cgagcggccg ccggtctcga cgccggcaaa tatcgccgcg gtgtcgttct ccgccgcgag
       61 cggccggggg attgatatga agttccagcc cgggaagagg gtgaggttgt cgggggaggg
      121 cgggggcggg ctatctccgg ccggagtgag ggggagacgg tattcgctgt tcgggatgac
      181 tctgccatcg gcgtcgcgct ggaggatgcc gaggaggata gggtcgtttg ttcctgcctc
      241 cggcaccgtg ccggagaggg tgaggaagac ctggatcttg ccccgaccat agccgagctc
      301 aaacgccgtc aatgtgaggg attttgcccc tcccgattgc gtatagacca ccactccatc
      361 acgactgaca gaaccggacc atgtggggtt ctcgagatcg gtcaggaagg ccaggtctcc
      421 ttgatattcg gcccagaagg tgctgctgag ggttccatac agcgtgacct cctcgccgga
      481 agcgaggtcg tcaagtgcgg gggttgtgga gatgcgctcc acccggtagg cagccccacc
      541 cgggggtgca gggtgggtga catcatcgta gatatcaaag actgcagtcg ctgatgccag
      601 accgttcggg gccgtaacgc ggagggtaaa cgcacccggc ccgatcgcgg ccgtatctac
      661 ttcaatgctc cagcttccat ccccggtcac aaccgtctca ccagagagag caaaaccctc
      721 ataatcctca attatttcgt aggtcagcgc tgtgcctttc cagtagtccg tcgtgccggt
      781 gaccgtgaaa gtcttcccgt aggtcgggtc gctgatcggg tctatctgga tccagccctc
      841 ttcgattatc ctgaaggaga acttcgtgta ggtgtccgga acgttcggag agtcaaggac
      901 gttgatcaat gcgcttgctg cagcagctgc ctgaaggccg gtaagataga tgggattgat
      961 acctggcccg ctgatcatgc cggttgccgg atccgccaca actccgggtg agtcacccat
     1021 cgggtgctgg acgatgacga agtactgacc tgcggcgagg ttttcagtat ctgaggactt
     1081 gagtttctcc tcgaaactgc catcgacctc aacgcagatc ggtctcgcgt acagctggtt
     1141 accattgcca tagaagttct tgccgaagat ccagacgtag acgttatccg gggcgcctgt
     1201 tgcacgcccg gagatcgtca gttcgtcgcc tcttgtgatg gcggcggcgc tcgcagttgc
     1261 cgtgacgaag ggagaccgga gctggatgta ggttgtcgcg tactgggttc cactgagggc
     1321 gttcttgcca cgcggctctg caaccgcata gattgcgtag ccgccggcgt caagcatacg
     1381 ggagagactt gaggtatccc accggtaaac ccaggtatca tcggcctcga cattcgcctt
     1441 ggtaaaggaa gctgcattgt tgttcgccac cgaggatata ttatcaagtc tgacgccgtt
     1501 gctgttgagg ttcggacccg tcaggaagag gtgggtggtg tcgccgtctg tgttcgtccc
     1561 ggagatgatg atctcctctc ccaggtagta gacgccggtg ccggaggtgg tgatagtgac
     1621 ggcaccgtgc tcgacccgca cccggacatc atcgtagatg gttgggtctg ccggatcctc
     1681 gacacggatg gtatatcgcc actcgccggt gctctggctg gtgttgaact ggatagttgc
     1741 agtgccggtg gcgttggtca tgatagtcct gttcgagtcg tcctccccgg gaacagtata
     1801 actcggaacc gcgatctggc cgggtacgat aaccgggtac gctcccggag ctacgccgct
     1861 gatctccctg acgtagagtc ggtagtcctt cttcgccgct ccggtgacgg tcacgacgaa
     1921 gcagtttccg cgaatgatgc tatccttgtt tgacacgatg gcaggtgctc tggagaccgg
     1981 tggggcttgt gttggtggcg gcgtaggggt cggggaggcc ccgaccggga tcaccctgga
     2041 gagggcctcg cacccatcga cgtagacagt gactgtcacg atatcctgct cgatcccctc
     2101 gatagcaaag aggattatgc cactgtcttg cccctgtttt gtgccgagca tcgtcaggtc
     2161 agcggtgatg ctcgatgccg ctgcctcccc gcggagggtg acgggactga tggtcccgct
     2221 ctcgatatta caggattgga cgatccgaac ctcgccgttc tgatcggcat acttgagtat
     2281 gatgacggtg cctccgtcgg ggaggtttac cgcaagttcg gtccagttcg ttcccctcgt
     2341 gtaagcgctc atctcaccag aattgagggc gaaggggagt gtgaggttct gaactgtaaa
     2401 aacggatgct tctcccggga tcgtagtaaa ttcactgcgg atgccaagtg cgaaggaagt
     2461 gccgtcaggt aagtcgtcca cattcacggt gatcgtgcca ccgggctcga tcgcatcagg
     2521 gctgataccg atcgtcgtcg ctgtcgtggc cgggatcaac agggtaatca ggaggagaag
     2581 tccacccagc ctcttcacgg gtaacaaccc ctcaattctc caccagcatt ggtaccggtg
     2641 ccgttcaccg tgattgctcc caccattgcc gctgtaccga ccagcgcagc ccggagggca
     2701 acggcgacaa ctatcccgat tggtcttcga tttatcataa tcccgctctc catagcgcgt
     2761 taccgggagg acctgcactg tacagaatgt tgactgtgaa ggcttgaatg aaagatggat
     2821 gtcgctcgac cagctgatgc cctcgctctg tgcgcaggct ttgatctcgt gtagggcagg
     2881 ataaaggtgc cgctattatc cagagcagca catcacggcc gggtgtaatt cagcatcgca
     2941 aaagagctgg ctctcctgta cggagggggg tcgccctgtt gcccggtggg agatcgacct
     3001 gaggggggag aactggttgc cggcgccagg cccatggtcc agtgatataa cgtcgccctg
     3061 gagtaggcga ggatagatga gctggatcac tgggccagaa gaacgcggaa tcgacgtaaa
     3121 aatagatatc cggccgcata tacggcgttt cggatggacg ggtgcaatgg tctagctggt
     3181 tatggcgccg cccgccccgg gcgacgtcgc aaccagctag accacaacgc ctgatacgct
     3241 caaccgccga actcttcggg aatttctaat agttgccggc gaaccgcacc ggaagagcga
     3301 tcgggaacaa cctgtatccc ggcgccccgc accctcctgg agcccacctg ccggcttctg
     3361 gtagccggat cgtagaacag aggagagttc ctgtaaccag gggcgccggg gagcggtcgc
     3421 cagaagattg tccgggaaca gcgacaaaaa ccggggtgtg gagaaaggag gttattgccc
     3481 gggcgtcacc cgagttcatc ccagccggtc ttcttcctgt agatgacgat acccactatg
     3541 accaggacga tgatgacccc ggcaccgata tagaggagga ggttgctatc gagattgaac
     3601 ccgtcaccga tcttctcgtg aagtgtgttt gcatcagtca gggcctcgtt cgccttattc
     3661 tggccctcgg tcgccttcga gcgcgccaga gggtagttgt tcgcgttcag gttgtctttt
     3721 gcctgggagt agaactggac ggcgctctcc cgcttggtca tgatggcgac cacctgcgga
     3781 tcgctcccca tcgagcggtt ttcgacgaag taggtgatca tgccgtcgag ggtctccagc
     3841 gtcgcggcgg cgttcgagac ctccttctcg gcccatgcct tatcgaggag cgccttcgcc
     3901 tcatcgatgg ccttcgtcgc ggtggcgata taccctgctg cctgcgatgg ccccgcggat
     3961 gcggcgtgag tgagatcctg attcgcggcg tcgtacttcg cctgcgtagc agcgacgtcg
     4021 acacccttgc cggctttatc atcgatatcg gccttcaggt ccttgaggcg ctgctgctgg
     4081 acggagagcg aacccgtaac ctgttgcgtg ctcacgacgt cacggacctt ggagtaggta
     4141 tcctttacgt ctttatcccg catatgctgg atcttgatca cctcgacctt tccggtcgtg
     4201 ctctcgggca cggtgccgcg tagctgaata tccagctcga tcttgccctt gccgtaggag
     4261 atctcaaact ccgtcagggt cgggtagtac ccggtcttcg tggtcctgta gatatcaacc
     4321 ccatcacgat agacgatgaa atcccaggta gggctctgca acccggtgct gaactcaaac
     4381 gtctcatcgc tcgcatcatc gggggtgatc atactgtagg aaagcacata gtgtgcagtc
     4441 acctgctggc cgggctcaag tgcgccgctc gcaggattaa tgtcctcttt cttgatgtcg
     4501 aaactcgccg ataccgcctg cacgacacag acgagtgcca gcaggagaat gatccatctg
     4561 ctgatgtttg ccttcatact acctcctcac tcaaacaatg gttctatacc gtcgtcgaac
     4621 atggtcgtac gcttctcttt cgacttcagg acatcctctt tcgtagcctt tgcaatctca
     4681 aggagttcgg tgatccgggg ggcgtttcct atcgtcgcaa gaaccaccac cgcagcgatg
     4741 tagtcgctct gcaccgggta gtcgccgcca cgcacctcga cgccggcgat gttctcctcc
     4801 acccagctct tcgacttctc gacgcccttc ctgtccatct cgtcgggcgg acccgcgagg
     4861 aggacaagac cccgctccgc cgtcgagtag tcgcacggca gggtaaggcg cccgagcatc
     4921 gcccgcctga cgagagcgat gatctttgcc gacttgtcct ctccggtcaa aacctcctcg
     4981 gtcttctcct tcttccgcat gaggccggag aggagcccct tgctctgctt cgcgcgaggg
     5041 ttgaccttct cgctgatggc gtatccgacg gtgctgatgc cgccgccccg cagggtgttg
     5101 atgacctcgc ttgagtccac gaccatctcg ccgacgccgg ccttgccgac ctcgcctgcg
     5161 cggaagagca cgccgaaccg gcggacgatc tcgtcgttca atcggttgta ggcgtccttg
     5221 acgctctccc cctcgttctt ccaggcgctg ttatcgaaga taaacgtatt atccgcttcg
     5281 ttcaccaggg tgctcagaga acgggctgca ttgtatgaat agagccggcc ttcctcgggg
     5341 gcgggcagga taccgatcgc atagaccggt tcgcggtaga tgcgcttcag gtgccgggcg
     5401 agtaccggcg agccccccga gcccgtcccg cccccgagac cggcgatgat cacgaaagca
     5461 tcgacgtcgt gcgtgcctct cgagtcgatg gcgttgatga tgctgtcgat ctcatcggcg
     5521 gtcacccgtg ctccggtcac gttgtcggtc ccgacaccgt gacccttgac taccgtctgt
     5581 ccgatcagga gccggtcttt aagttcgata tgtttgagcc ccatcaggtc ggtacgcgcc
     5641 gtgttcaccg cgatacccct gaaactctgg gtctgcatcc gtttatcctg ctcgatgaac
     5701 atatcgacga ctttgccccc cgcctgaccg aatcctatga agaagaccct cattcagctt
     5761 cacaccctca tatagcctaa tcaataatat cgacgtttat gtataaagac gttgtatact
     5821 cttatagccc aatagtgttc ttataacttc acacgtagtg gtaagcgatg aaggtctgca
     5881 ttgtgggggg aggattatgc ggtctggtct cggctctcga acttgccggg gcacaccagg
     5941 tagacctctt cgaacgacgg ccggttccgg gaggctgtct tggttcatac cggatcggcg
     6001 attactggat cgaggaatat taccaccatt gcttctcggg cgacaccagc ctgctcaatc
     6061 tgttcgacac ccttcaggta gccgaccggc tggtatggct caagggcacg accggctact
     6121 acgtcgacgg gactatccac ccgctcacct ctcctgccga gatcctgcgc tatccccatc
     6181 tcacgctcat cgagaaggcc cgcctcggcc tgctgaccct gcgctcccgg cagctcgacg
     6241 ctccggccct cgacgggatc accgcaaagg agttcattct cgataacctc gggcccggca
     6301 tctacgcctc gttcttcgaa ccgctcttga agagcaagtt cggggaccgc cgcgaagagg
     6361 tctccgcagc ctggctcata tcccgcatcg cgatccggtc cgaccggggt gcgggcggcg
     6421 agcgtctggg ctacctccgg ggagggttcc ggcacttcgt cgcgcggctc gaagaagagg
     6481 cggcaaaccg gggcgcctcg atcatgcccg actccccggt cagggagatc cggcgggagg
     6541 gcgacggctg gctcgtcgac ggcgaggtct acgactgcgt cgtgtcgacg ctccccccgc
     6601 aggtgactgc ggaccttgcc ggcctcgata tcgccccggt gccctaccag ggcgccgcct
     6661 gcatgaccct cgcactcgac cgggacgtga ccgacggcat ctactggctg aacatgaagg
     6721 atgccgcccc atacggtgcg gtggtctcgc acaccaactt tgccccgttc gagtggtacg
     6781 gcgagcatat cgtctacctc gcgtcttact tctccggccg gctcccggag ggactcgagc
     6841 agtcgatgct cgcggacttc tgcaggcggt tctttgtcag cccggacgaa gtgcactggc
     6901 accggctcgc ggtcgacccc cacgctggtc cggtctacac gaccggtctc cggagccgcc
     6961 tgcccgccta cgaggagcac gggctcttcc tcgccggtat gttcagcccc ccgaactatc
     7021 ccgagcggag catgaacggt tcggtcgtcg ccggcaagga agcggcaaag cgggttcttg
     7081 cgaggtaccc cgatgtgtga agtcgaggtg agcgccgtcc tgccggtcta caacgatcta
     7141 tcggcgctca aggtcgccat accgcggtcg cttgagacgc ttgaggcgat cgcgccggga
     7201 ggttttgagg tgatcgtcgc cgaggacggg agcaccgacg ggagcgcgga gttcgtcggg
     7261 gcgtacgagg cagacgatcc ccgggtccgc ctgctccacg ccgacgagcg tctcgggcgg
     7321 ggccgggcgc tcaaccgcgc gtttgccgcg gcgaaaggat cgatcgtctg ctactacgac
     7381 gtcgatctcg ccaccgatat gcagcacctc cccgaactgg tcggcgccat ccgggacggc
     7441 tgcgacgtcg cgaccggatc ccgcctcctt gcggagagca ccatcacccg gagcggggga
     7501 cgggagatcg cgagccgcgg ctacaatatg cttgtccgga cgatcctcgg gagcagactc
     7561 cacgaccacc agtgcgggtt caaggcgttt cggcgcgacc gccttcttcc cctgctcccg
     7621 tcggtctccg caggccactg gttctgggac accgaggtgc tggtgcgggc acagaagaac
     7681 ggctaccgta tacgggagtt ccccgtgcgg tggcagcagg gagaggggac gaccgtgcgc
     7741 aaaaaagacg tcgccgagat gggctcggcg gtcgtgcgcc tctggtggca actccatgtg
     7801 gaagaaggtt agcacgatcg tcatcccgac cctggtcgcg gtcgggatcc tcgcctacat
     7861 gctttacagc gtatgggaca atctcctgct gacgctcgag catgcagtga tacccttcct
     7921 ctttgccgcc atcggtatct gcgttatcgc ctggatcctc cgggggtttc gataccagtt
     7981 catcctggag ggactcggcg tcccggaaag cctcaggttt gcgaccgcct gcatattcat
     8041 atcccagacg gcaaacctca tcgttcccgc acgcctcggc gacttcgtgc ggatgcttat
     8101 cctcaggcac gaggataacg ccacctactc gcaggggttc tcgtccatcg tcgtcgagcg
     8161 cgtcttcgat atcctgatgg tcgcggtcct cggcgccata gcgctcccgt tcgtcctcgc
     8221 cgtccccgac tggttcatca cggtcatcgt cgtccccatc gccgcgggag gcgtcttctt
     8281 catcgtgctc ctctggtcgg gcaggatgaa gtctgagaac cggttcgtcg tcgccgtcca
     8341 gaggatgctt gacgaggtga aacaggcctc cctcaacccc cgggccctcc tcatgctcag
     8401 cagttcatcg ctcctcatat ggctcgtcga tgtgctggtc tgcgccgcgg tcgtcctgat
     8461 gttccaggaa ccggtcccgt tcgccatcgt cgtcctcgct atcgtcatcg gcaacctcgt
     8521 caaggcggtg ccgatcaccc ccggcggcgt ggggacctac gagctcgcgc ttgcactgac
     8581 cttcgggctc gcgggaacgc cggcggccac ggcgacgctg atcgcggtca tcgatcacct
     8641 catcaagaac atggtgacgc tcgtcggggg catcgggtcg atctactact tcggcgactg
     8701 gtcgatggac ctcttaaaga aggtcttttc ccgggagatt gataaggagg agacgtttgg
     8761 cgggtgagtt catcgtcccg gtgctcgcct ggcttgcggt catcaaagcg ctccacctcg
     8821 gcacctggcc ggcgctcgac cgcaccctcg gcagcctcgc ggccgccgcc gcatacccgg
     8881 catcggttct tctcttcacg ctcctctcct ggtactgcgg catcctgggc cttcctatat
     8941 tgctcgccct cctcccgttc cttgccggca tcgcccttgc cgggtcgcgg gggttctaca
     9001 cgaaggagcg cctgcgctcg acattcgcct gggatctcgc cttcctggtc cctttcctct
     9061 tcatgctcga ggtgcgctgg atcaacccga ccatctccta cgccgagaag ttcatggacc
     9121 acgcgttcat cgcttcgatc atgcggcaac ctatcgtccc gccgctcgat ccctggtatg
     9181 cgggaggggc gctggacgtc tactactaca tcggctactg gatgaacggg atgctcgggc
     9241 ttgcatccgg cgttccctcg acggtcacgt tcaacctcgc cctcccgacg gtgctcgggc
     9301 ttgccgtcgt ctcgctctat gccctcgggc acctcctcct cgaccgccac cggtggctcc
     9361 ccgtgcttgc gcttctcata ccgaacccct cggcgatcta ccatatcgtc atcgggaact
     9421 cgtggttcga cgtcctctgg gggagcaccc ggacgatcaa ggataccatc aacgagtacc
     9481 ccatcttctc gatgctctgg ggcgacgtgc acccgcacgt catcagcatc ttcaaccagg
     9541 ggttcctgct cttcctcctg gtctttgcct acatgcggtg ggggacgctc accatgcggg
     9601 gccgggttat cctctgcggg cttgccgccc tctccctcgg gtccatgccc gggatcaact
     9661 cctgggacgt gctcatctac gccccggtca cgctgatctt cggcctcctc gtatggcgac
     9721 gttacggggg ctccgcgctt gacgatgaac ggtcctggat gatgcttgct gccgtgccgc
     9781 cgctcgcggt cgcggcctac cttccgttct acctgcagct tggcagttcc ggcatcgggg
     9841 gaatcggaat cgttccggca ccttcagatc cggtagagtt cctgctggtc cacgggttct
     9901 tcctcgcgat cctcgtcgcc tcatctgccc cggatatcag gagacgcccc tacctcctcg
     9961 ccgccgcggt gccgttcctg ctcgccggct atcctgccgc agccatcgcc gtcgtcccgc
    10021 tggcgtacct catcgcgcgc cgccgcttta cggctgccga tacgctcgcc atactcgggc
    10081 tcggaatcat cctcgcgacc gagatcatct atctcaagga caacatgggg gagacctact
    10141 tccggatgaa cacgatcttc aagttctacc tccctgcctg gctcctcatg ggaacggcaa
    10201 gccttgcgat cgtcggggag tggctcggga atgccggtat cgacgggcgc atgcctgccc
    10261 gggtggagag ggtgctgccg gccctggccg tcgtcgctct ccttgccatg ccctttgcga
    10321 tcaacatcga cttcggttac gggagccaca ccctcgacgg ggcggcgtac ctcgaggggt
    10381 cgcaccccgg ggatgcagcg gcgatagcgt ttctccggaa ccttccgggc gaccagatca
    10441 tcgtcgaggc ggaaggggga gactatacct actactcgcg ggtatcgtcg ttctccggga
    10501 taccgaccat cgtcggtatg ccgtttcacg agtacatgtg gcggggagac gcaggacgca
    10561 taaccgagcg gagcgtcgac gtgcgagcga tatacgagca gccgtcgcgg gccgtcgacc
    10621 ttgcgcgggc gtacaacgcc accctcctct acgtgggaga cgaagaacgc gaccgctatc
    10681 gggtatcgat accggcagac gtgcttgagc tcatctacga tgcagaggga gtccagatct
    10741 accggatccc cggataacgg catggccgca cccacacacc cccgcccgga aacgaccgaa
    10801 tcagatttcc ctgcggcagg gccgaatgac aaacctttat cactcctcct attgacataa
    10861 tacagcgcat tcgatcagct acgctactga tgaatgatga cggagcagcg agacctgctc
    10921 ctgaaagagg cgagttatgg caaaggcata tgtaaaattc gagattcccg cagagatcca
    10981 gaataaggcc cttgaggccc ttgagatcgc aagagatacc ggtaaggtaa agaagggagc
    11041 aaacgaggca acgaaagcaa tcgagagaaa cattgcccag ctggttctca tcggttcaga
    11101 cgtcgagccc gaagagatcg tgatgcatct gccgccgctc tgtgaagaga agcagattcc
    11161 tttcgtctac atcacgaagc agaacgacat cggttctgca agcggccttg aggtcggctc
    11221 ggcagccgcc gccatcgtca agcccggcaa ggcaaaggat ctcgtcgatg agatcgcaaa
    11281 gcagataacc gcactgagag cgtgagggag aagagatggc aaacgacgga acgcccgcag
    11341 aagtcatcga gatcatcggc atgaccggta tgcatgggga agcccagcag gtgaaatgcc
    11401 gtgtcctcga cggtccgaac aaggggcgga tcatcacccg taacaccgtg ggccccatca
    11461 gggagggaga catcctctcg ctgcttgaga ccgagcgcga agcaaagaaa ctctcgaggc
    11521 ggtaacaccc atggtcgaca gatacatctg caatttctgt ggagagcctc tcgagcccgg
    11581 caccggcaag atgttcgtca ggagagacgg gaccgtctac tacttctgca gcaccaagtg
    11641 ccagaacaac tacaggctcg gccgggtgcc gcgtcgcgtc gagtggacag cggccggtcg
    11701 gaaagcccgc ggcaaagagt gatccggcat ggatcgcacc tttgtgatgg tcaagcccga
    11761 cggcgtccag cgcggactcg tcggggagat cgtctcccgg ttcgaggcaa agggcctcaa
    11821 actcgcgggg gcaaaacttg agctcctccc cgagggacgg gtcgtcgagc agtaccggga
    11881 acacctggag aaaccgttct tcccgggcct taaggcctac atcatgagcg gcccctgctt
    11941 cctgatggcc tgggagggga gaggcgcggt cgcggtcgtc cgcaggatgg tcggcgccac
    12001 aaaccctgca gaggcggctc cgggctccat acgcggcgat tttgctctcg acatagggcg
    12061 gaacgtgatc cacgcatccg actcccccga gagtgccgcc cgcgagctcg gcatccactt
    12121 ttcgccccgc gaactggtcg aatactcgag gatcgacgag cccgtcctct acgagtgaat
    12181 accggcggaa aaaccgtttt cctctttttg aggtccggcg agcaagatcc gggcggccgc
    12241 ttcggcccaa aagaggagat cttcttcgcc cgccggaaag catctttctt tgttccgcgt
    12301 ttttcccgaa acaaatcgtt tgtcctgccg gcaaaccaca agtgctaata tcaaacctac
    12361 cgcatgcctg atcacaaatg gccgatctcc tgagttttgc gctgctgagc ttctcttcga
    12421 tcgtcatcgt agtgaacccg ctcgcagcca ccctgatctt cgtctccctc accgggacga
    12481 tggatggggc aacaaaactc aaggtcgccc gcgatgcgag ccggttcgcc ctggtcgtcc
    12541 tgctgatctt cactgtcgcc ggcggctgga tcctgcagct cttcggcata tcgatcgagg
    12601 cgttccgcat cgcgggcggc ctcctcctct tcgggatcgg catggacatg gtctacgcaa
    12661 agacatcgcg gacgaagatg accgcgaccg agaagtacga ggggcaggat gcagacgaca
    12721 tcgcggtcat gccgcttgca atcccgatga tcacggggcc cggcgccatc acctccgcga
    12781 tcgtgctgat gaacgaggcg atgagcatgg gtatcgccgc actcgccgtc gtgccgatcg
    12841 ccgccggcgc ggcgatcgcg atcacctact acatgatgca gcactccgat atcatcgtcc
    12901 ggcggatcgg gcaacgagag taccgggccg tcaaccggtt gatgggtatg ctcctcatcg
    12961 cgatcgcgat ccagttcatc ctcatcggga tcagggcggc gttccccata ctcaccggag
    13021 gccctgcgtg acacccgatc ttacgtttcc cggcatggtc ctgatcagtt cgagcatcat
    13081 cgtgggcggg atcctcatcg gagctctgac gttcgcgatc gacgttccgt acgcctcctg
    13141 caccatggtc gtgctcgccg ccctcatcgt cctcggcgcc accatgctcg tgctctccac
    13201 aagaaaccag ggcggggttc cctcccggtg atgacgaaga tagcgcttgc ccaggttctc
    13261 ggcggtgccg cccgggcact cgatacggcc ggccggatgg ccggggaggc cgctctggcc
    13321 ggggcgtcgc tcatctgttt tcccgagcag ttcgtggccg ggtggttgcc ggagatccct
    13381 cccgggtccg gggaacccct ggatggaccg ctcgccacgg cattcgggcg gatggcacgg
    13441 gagagcggca tcgcggtcgc ggggtccatc atcgaggcgc gagggggagg ccggcctcaa
    13501 aataccgtcg tgatcctcga cgcgaacggc gagctcctcg ctacctatgc gaaaatccac
    13561 cttttctcgc ccggcgggga ggaccgcaac tacacgccgg gcgaccgggt tgccacgttc
    13621 accatcgacg ggatgacgtt tgggcttgct atctgctacg atctccggtt tcccgagatc
    13681 ttccgaatat atgcactcgc cggggcggaa tgcgtgctcc tgccggcggc atggccgtgc
    13741 cgccggttgc gccactggga gaccctgatc cccgcacgcg ccctcgagaa ccagtattac
    13801 gtggcaggag tcaacacggt cggcggggtc tactgcgggg gatcgcttgc cgcaggcccc
    13861 gacggcgata ccatcgcccg gggcgggtcc ggggaagaac tcctcttcgt cgacctcgac
    13921 ccggccgcta tccacgaggc ccgcatgaga ctcccgacat tttcagaccg cagaagcgac
    13981 ctttatcaca gattgctctc taatctatag agacatctca tgcggggcga ccagcacgta
    14041 tacctcagtc tcacgacagc aggactcctc atcgcaccct tcgtccccgt cctcgacccg
    14101 gccctgatcg tcaccctcgt cttcggtgtc tttgtcggtt cgcttgcacc ggatgcggat
    14161 gccgtcgatg cggcgatctt caacggctgg ataggcgggg cgaaggggaa gagaggacag
    14221 gtcttaaacg gtgttgccgt agtgctcccg gtctttggct acaccatccg gtacctcatc
    14281 tactacccgc tctcccttgt cttctcgctc atcctccgaa agagttaccg gcaccggcac
    14341 cgcggtctgc tccactcgct cgcgggcgtc gggttgacat ccgttatcct ctccgtctac
    14401 ctggcgttca tcctcacctg gattggatgg tctcctgcac tcctccccgc ctttggagtt
    14461 gcgttcttcg ccgggtgcat cctccatctc acggaagatg cctgtacacc tgccggcgtc
    14521 gcctggctct accccttctc ccggcggcgg atggccggcc gcatccggcc atggggaacc
    14581 ttcgagatac gcccggcagc cttcgccgcc gtgctcgccc tggcggctgc cggcgtgttc
    14641 atcgcgccat tcgtgaccgc cgtatctccc ggagctctca ggtgcctcgc cctcgccgga
    14701 accctcgttc tctggctgct cttcatgctc gtctgccgcg tccggtgcga gcggcgcagg
    14761 tgagccggcc ccgatccgcc ataccatatc tatacgtatc cgggcgatca accatttttc
    14821 catgtaccgt ctcgtctgcg ttcactgtgg tgcaacctac accccggacc agatcatcta
    14881 tacgtgcaac cgttgcgggc acctgctggc cgtcgaatac gacctggacg gcatcgacgt
    14941 ctcccgcgac gaatggaacc ggcgccccct ctcggtctgg cgttaccgcg agctcctccc
    15001 ggtcgggggc gaacccatca gcctccagga ggggggaacc ccgctctatc acctgaagaa
    15061 tatcggcaaa gaactgggcc tccccgaact ctacgccaaa cacgagggga tgaacccgac
    15121 aggatcgttt aaggatcgcg gcatgaccgt gggcgtcagc atggcccggg aactcggtat
    15181 gacgagcgtc gcctgcgcaa gcaccgggaa cacctccgcg agcctcgccg cctacgcggc
    15241 gaaggccggc atcccctgcg tcgtcctcct gcctgcggga aaggtagccg tcggcaaggt
    15301 cgcgcaggcg ctgatgcacg gcgcacgggt catatccatc cgggggaact ttgatagggc
    15361 gcttgagatg gtacacgaac tctgcatcag ccagggcctc tacctcttga actcggtcaa
    15421 cccctaccgc ctggaggggc agaagacgat cgggtttgag gcaatcgatc aactcggcgg
    15481 cgacgtgccc gaccggatgg tcctccccgt cggcaacgcg ggcaacatat ccgcggtcta
    15541 caagggtctc cgagaactcg aggccctcgg gttcatcgac cgcctgccga tgatgaccgg
    15601 catccaggca gccggctcgc agccgctcgt gaaggcgata gcagagaacc tcgacgaact
    15661 catccccgaa cccgcgcccg agacggttgc gactgcaatc cggataggcg ccccggtgaa
    15721 cgcggagaag gcgctcgtcg ccgtccgcgc gaccggcggg acggcggcgg ccgtgaccga
    15781 cgaagagatc cttgccatgc agcgagacct cgcgaggaag gagggcattg gagtcgagcc
    15841 tgcctccgca gcctcggtcg cggggatacg taaactcgcc gaacagggtc tgatcgataa
    15901 ggatgagcgg atcgtctgcg tggtgaccgg tcatctctta aaagatccgg agacagtggt
    15961 acgacaatgc gttcctcccc tcgagatcga cgcggacctg cactcgctgc tatctgcctt
    16021 gcgctgatcc tgatctgcac cccggcggtg gcggcaaaag aggccacttt tcgggtgctc
    16081 cccggcggca ccacctatga ggcctcggtc caggttaccg gaacaggcta cggattctgg
    16141 ggcccgggcc tgatggggga acgggttccg ctccaggtcg aggatctcaa ggtgcttggc
    16201 cccgcgggtc cggtcgatta ccgggaggcg gggagaggcg ccatcgcctt cccggagggg
    16261 aactacacga tcacctacca ggttcccgtg agggataacc atcttgttgc gatcttcgat
    16321 acgccgtatg ccgtaaccgt tgcccttccc gaggggctcg acgtcaagaa tccgctcatc
    16381 ggtatggtca gccccggcgg ggccgtctcg cccggaccga acgagaccat ggagataacc
    16441 tgggaccgga cagatgccgt ggaggtccgt ttctacgccc ccgagcgcga gatcctgctc
    16501 accacattcg gcaccatatg gctctcggtc gccatcgttc tgctcctccc ctacttcata
    16561 tcgtggagga gagacggcgg atgatcgata tcgtcgccgc atcgttcctg atcggcctct
    16621 ccggagccgc atcccccggc cccatgaccg cctcgatcct cggcctcggt acacggcgac
    16681 ccgggccgtt cgttgcgggg cttatcgcgg ggcacgctgt cccggaggcc gccatggtca
    16741 tggccatagc ctacggcgtg cgtgacatcc cgtacatcga tctgatcgct ctcatcgggt
    16801 cgggcgtcct catagcgttc ggcgtcagcc agctcctcca cgccggcgat accctcgccg
    16861 cgagcagaga gacccgtgcg ccggtcgccg tcggggtggc ctgcacgctc ggcaaccctt
    16921 actggtgggt ctggtggctc acgttcggcg tcgggtttct cgcactccac ccgtcgtttg
    16981 ctgcgttcta cgtcggacac atcggcgcgg atatcgcctg gctcgggctc cttacattcg
    17041 cggtctcacg gggagcaaac gtgctcggcc ctcattataa aaaagtggtg caggcgagcg
    17101 gactcgccat ggttctcttc gggctgtact ttattctgac ggtgtttttg ccttgatgtc
    17161 gatcccgaac cgctcggcgt tcgcatcggc aattgcctgg atatgcgcga tcacgtcgtc
    17221 ctgccgggcg ggcttgaaga ggtggcggaa gcgcttctgt tttgcgaggt actcctcgac
    17281 cggtttgcgc ctcaccttct ttaccttctc caccttgccg ttgactatct cgtagttcac
    17341 ccagaggccg gtatcgacgg cgagtttacc catcgcgagc gtctcgccgg actcaaaacc
    17401 ccaccccgtg cagcaggggg tatgcacctg gatgtagcag gggccggggg tgttgatcgc
    17461 gcgttcgacc ttcttcatga agtcggccgg gtaggcgacc gaggcggtcg cgacgtaggg
    17521 cgcaccgtgg gcggcaagga tcgcgggcat atccttcttc gggagcaggt ttcccggcga
    17581 gcacgtgccc gcaggactcg tcgtggtgct cgcgccgtag ggcgtcgcac ccgaccgctg
    17641 gatcccggtg ttcatgtagg cctcgttgtc gtagcagatg taggtgatgt cgtggccccg
    17701 ctcgaaggcg ccgctgatgc agagcacccc gatatcgaac gtggctccgt cgccggcgac
    17761 gcagacaacc ttttcgctgc gcccctgctt cttcagcgac gcctcgatgc ccgatgcaac
    17821 cgccgcaacg ttctcgaaga gcgagtggat ccagggaact tcccaggcgg tctcggggta
    17881 gggtgtggag aagacttcca tgcatcccgt cgaggcggcg atgatgacgt tcttcccggt
    17941 cgccttgagg aggagccggg ccgcaagcgc aggtccgcat cctccgcacg cccggtgccc
    18001 ggccgagaag agttcgcatc cctgctcttt ttcggccatt cagagcacct ccgtccgtaa
    18061 tccgtaaaac atatctcctt tccccttctc cgcgagatcg acgaccgcgg cgatatccct
    18121 cttcctcaca tcgcgcccgc cgagggcgat gatgtagtca tacacggtca cgccggagcc
    18181 gtagagcgcc gacttcacct cggtgccgac cgccccgccg ttcccgagcg atatgttctt
    18241 gtcgagcacc gccaccgtcg agacaccctt caaggcatcc acgatctccg gagcggggaa
    18301 cggacgatag gtccggatct tcaagagccc gacctttttg cccgcatcgc gcatctcgtc
    18361 gatggcatcc ttcaccgtgc cgcagatcga acccatcgcg atgagcgcgg tatcggcgtc
    18421 ctcaaggcgg taaccctcga cgaggccgga gtagtcccgg ccgaactgtt cgccgaactc
    18481 gcgcccggcc ttgatgaacg actgccttgc gcgctgcatc gcccggtcga tctcgtagcg
    18541 gaactccatg taatactcgg gcgttgcata catacccata ctcagcgggt tcgcggcatc
    18601 cagtcgctgg tagggtgtaa aggcaggcag gtaggcatcc acctcctcct gggtggggag
    18661 ggagaccggt tcgtaggtgt gcgtgagaat gaacccatcg aagcagacga acgccggcag
    18721 gaggatgtcg tggtcctcgc agaccttgta ggcgatcatg tggaggtccg cggtctcctg
    18781 gttatcctcc gcgtagaact gcagccaccc ggagtcgcgc agggatatcg agtcctgctg
    18841 gtcgttccag atcgagagcg gcgcgccgag cgagcggttc gcgatcgtca tcacgatcgg
    18901 ctggcgcatc cctgccacgt tgaaacagac ctcgaacatc agggcaaggc cctgggaggt
    18961 cgttgccgag tagacccggg agcccgccgc gctcgcgccg aggcaggcgg agagggccga
    19021 gaactcgctc tcgaccgtta tgtactcggc gtcgagttcg cagttcgcca ccatctgggc
    19081 gaggtcctcg acgatgtgcg tctgcggcgt gatcgggtag gctgagatca cctgcgggcg
    19141 gcagcgcttc acgatctcgg ccaccgcatg cgacccttcc ataaactcca gcattacttc
    19201 tcctccttct ccatccggat cgatttcttc gggcagatct cttcgcagat cccgcaaccc
    19261 ttgcagtagt cgaggtcggg ctcgaacgtg ccctcttccg tctcgcggac gcacccctcc
    19321 gggcagacca tggcgcacat gccgcaccgg ttgcatgtct ccggatcgaa gaccggtttg
    19381 aagaccctcc aggtaccggt cttgttatcg cgcgctcttc cgggaggcgc agcgcagccg
    19441 actcggagcg ccattatgca gcacctccga tcaggttgta tgcacgctct gccgcccgga
    19501 tgttcttctc ggccatactc ccggagaaac gcctctgaag tgcatgtttc agcggttcaa
    19561 gcccgatctc gccggtggct gcggcaaaag cccccatcag ggtcgtgttg gtgatgggca
    19621 cgcccagttc ctcgagcgcg atggtggtcg catcgacggt atagaccttc acgccctccg
    19681 ggacccggga gtcgaagtcg ggcttctcgg tgttgacgat ggcaataccg cccgctttca
    19741 tgcccataaa gacgttcaca tcgccgatca gggtcgagtc ctggacaatg atatagtcgg
    19801 gctcgtatat ctggctccgg agccggatct ttgcatcact gaagcgaacg aacgcctgca
    19861 ccggggcgcc ccgccgctcc acgccgaagg ccgggaatgc ctgggcatac gcgccaccct
    19921 caaacgccgc gaaagcgatg agctcggcgg cggtcacgga gccctggcca cccctgccat
    19981 gaatgcgtaa ttctttcaaa caaaaacccc ttcacaatct taatgattaa taatataaat
    20041 acatcggcac tttgcctgcg gccggccctg caacaccgcg ctcccaggag ggcgcgggat
    20101 ctcccgctca atccccggga gcgaacccgc ggtaaaaacc tgccgcaatc tatcggcacc
    20161 gatgagaatc aatccagtta aatataaatc attcacaaaa gacaaatgtt aataattggt
    20221 gattgctatg gtaaacaatc ctgtgagggt atccaggggc ctggtgccga tctgctgcat
    20281 cctcatcctt gcctcattct gcgtcgtgcc cgcctgggca agcgatgccg ctgtttcccg
    20341 tgagatcgac cgttcttcgg tcccggtcgg cggcgaggtt ctggtgacgt tggcggtcga
    20401 cgatctcggc gtcggcggtg tcgtggagac gataccggag ggttttgtct tcctcgagac
    20461 atcctttccg gaggatcgtt acagcgtatc cgggcagaga ttgacgtttg cggtgatcga
    20521 tgaacgcaag atcacctacc gggtccaggc aaaggccgcc ggctcctgga cgttttccgg
    20581 aacgtgggtt gacgccgtcg acaagaccga aggagcgatc cccccgactg cggtgaccgt
    20641 cgggtccggg gcagcggcag ccggcgcagc gcccgcgggt accgggcagg cacccggccc
    20701 gaccagtgcg ggaggcgcgc cctgggcggt cgtcccggct cttgcagctg cggccgtcgc
    20761 tgccggcgcg tgcgccgcac gaaaggagcg gtgacccgcc gatcatgcat tacaccgtct
    20821 cttccaccca gtggcccgac cgcacctcga ccatcgtcgg cgccgcgggg aggggcggcc
    20881 tttccggggg tgtgcaggct cgtcccgcaa acgcggcatc gacgagcctc tgggtgtagg
    20941 agtggcaggg acgatcgaag acgtcggcgg tcctgccgat ctccacgatc cttccttcca
    21001 gcatgacggc gacgcgactg ctcatcgccc gggtgacgcc gaggtcgtgc gaaatgtaaa
    21061 ggcaggtcat gcggtagtcg tgcccgaggt cgcggatgag gttgaggatc tgggcctgga
    21121 cggagacgtc cagggccgac gtgggttcgt cggccacgag aaacctcggt ttgagagcga
    21181 gaacgcgggc aaggaccacc cgctggttct ggccgcctga gagttcatag ggccggcggt
    21241 tcaggtgctc ctcgttcaac cccacctctt cgatcagttc ccgcaccctt cggtcgatat
    21301 cggccctgct ccccatccgg tggatcttca ggggctcggc aacgctctcg ctgacccgca
    21361 tccgcgggtt gagcgaggcg taggcgtcct ggaagatcat ctgcatccgt ggccggaagc
    21421 gcctgagttc gctccgggag agggcggtga cgtcggtccc gtcgaccacg atccggcccc
    21481 tgtccggctc gatgaggcgg aggatcgaac ggccgagcgt cgtctttccg cacccgctct
    21541 caccgacgag cccgagggtc tcgccctcct cgatccaaaa cgataccccg tccaccgccc
    21601 ggaccaccct ccggtggatg aagcccgtcg agaagttggt ctcgagatca gtaacctcaa
    21661 tcatagagca tgcaccgtgc ataatgcccg ggcccgacct cgacaagtcc ggggtgttct
    21721 ctgttgcagc ctccgatcgc gcatccgcac cggggtgaga accggcatcc gggcggcagg
    21781 gtgatgagcg aaggggccga gcccgggatc ggtcgcattc cccgttcagg gagcgaggcg
    21841 aggaggcccc gggtgtacgg gtgggccggg tctccaaaga cctctcttac cccgcctacc
    21901 tcgacgatct cgcctgcata catcaccgcg acccgggcgg cgaggtcttc cacggtctcg
    21961 atgtcgtggg tgatgaagag gacggagcgg tcccggtcga tctccgagaa gaggtcgacg
    22021 atcccccgct tggtgacggc gtccaggcct ttcgtgggtt cgtccgcgat gaggagggtc
    22081 ggacggcacg cgagcgcggt ggcgatgagc gcccgctccc gcatcccccc gctgaaccgg
    22141 tggggatact ccccggccat cctgaccgga tcgggtatcc cgacggcgtc gagcagttcc
    22201 accgcccgct gccgggcgtc gggcccgctc aacccctggt ggagggtgac cgcctctgca
    22261 atctgggtcc cgaccgtgta taccgggttg agggaggtcc ccgagttctg gaggacgatc
    22321 ccgatctccc gcccccgtac cctctgcatc tcggcctccg gaagggagag gagatcgcgg
    22381 ccgcggtagc ggatctcccc ctcgatgatg gtgttgggct ggaggagacg aaggatcgcc
    22441 atgccgagca cggtcttccc cgacccggtc tcgccgatga gaccgagggt ctcccgcggg
    22501 tatatctcga gatccacggt atcggaagcc cggaccagcc cttccggagt ggggaaccct
    22561 actttgagcc cccggacttc aaggagtgcg tcgctcatcg ccgtcatacc cgcatcaccc
    22621 ggagtctcgg attgatctcg tcttcgcagg cataccccag ccacgcaaga gcaagagaga
    22681 tgagcgtcac cgcaaggccc ggcgggatca gccaccacca gaggccgagg atcatcgccc
    22741 cgttctggct cgcccggttg atcatctgac cccagtccac gacgctcggg tccgtgatgc
    22801 cgaggaatcc gaggccgatc ccgatcagca tgaagtgctg ggcggaggtg acgaacttga
    22861 cgccggccag ggggaggacg cccggaagga tatgtcgtgc catgatgtag ccgtcgccgg
    22921 cgccgagcgc tcttgtcccg acgacgaagt cggcgaccgc cagggagagg acctgcgacc
    22981 ggatcaaccg cacgccctgg aaactaccta aaagacccat aaggaccgcg atcaggtaga
    23041 tccccggccg aaagaacgtc gtgaggatga gcaggagcgg aaagatcggg atggtcatcg
    23101 ccacgtcgag gaggcgcatg acggcttcgt cgatcaggcc gccgtagtac cccgacacga
    23161 tgccggcggc ggtgccgatg gcgaccgcga tggcggctcc gatgaaaccg aggagcagcg
    23221 tcgcccgcgt gccccatatg agcccgctga agacgtcctg ggcgaggtcg ttcgtcccga
    23281 gcaggtggtc tttgtcgggg ggggagagcg gcgcgcccgt gatcgcgtga gggtcgtagg
    23341 gcgcgatcac cggggccagg agggcgaccg cgcagtacgc gaagacgatg ccgaagcttg
    23401 cggcaaggat gaccttcttc ctaggcatcg tcacccacct gcacgcgcgg atcgacgagg
    23461 gggtagatca ggtcggcggc gatgttcgcc gcgatgacga agagcgtgtc gatcagcagg
    23521 atcccctgga gcagggggag gtcgcgggcg gcgagggcct tgtaggtgag catcccgagg
    23581 ccgggccagg agaagatcgt ctcgacgaag atgctcccgg tgatcatgcc cgcgcactcg
    23641 aacgtgatca gcgtcaccat cgggagcatg gcgttcttca gggcgtgctt cctgaggatc
    23701 atcagttcgt cgagcccctt cgccctggcg gtggtgatgt agtcttcccc ggccgtcatg
    23761 agcatggagc tccgcatgag ataataagtc ccggtcaccg agaagaggaa gagcgtcagg
    23821 accgggagca cagcgtggtt tgccacgtcg agcggatcga tcccggtggt gagggcgccg
    23881 gtatcgacgt acccccccag cggaaagagg ttgaggtagt aggcgaagat gatgaggatc
    23941 atgagggcga gccagtacga ggggatcgcc cggatgatga tcatactgtt caggagcgcg
    24001 atgtcggtct tcgatccgcg tctccagccc gagtacgcgc cgatgccgat gccggcaagg
    24061 gtggcaagga tcgttgccgg gaggaggagg atcagcgtcc agacaagtct ctcgccgatc
    24121 acggtgagga ccggaacctt gtagatgaag gagtatcccc agttcccccg gaaggcatcc
    24181 agaaggtaga tgacgtactg ctccaggatt ggtctatcca gaccgtatac atggttgaat
    24241 gcttcaacga cgtccggggg ggcgtaccgg tagatcgcct ccaggtacat ggtgatgtag
    24301 tcgccgggca tgagacggag gagggcaaac atgatggtca cggcgatgca gaaggctgca
    24361 aacgaggtga tcagccgttt gaggaggtat atgccgttca tgataatggc caggggatct
    24421 ggtgaaaggg gaggggacga ggcctgtacc ggcgaacgat cacgccctca gccccacctc
    24481 ggcgctcgcc ggtctgatct cggagaagag acggatgttc ccgccgccgc cgagcagggc
    24541 ggcgtcccac ccgacgaacc gatccgaccg gtaggccatg aaggcgtcct gggagaagac
    24601 cgggatgcag ggcacctctt cggcaaggat ctcctgcagc tcaaatccga tctcctccct
    24661 tttatcggga tcggccgtgc tcttcaactc ggtgatgagc ccgttgaagg tctcgttgtc
    24721 gaagtggtag tagttctcgc cgagcgggct cctctggaag ttgtccaggc ggtcgatgtc
    24781 ttcatgccag cgaccgggca tgccgtcgaa gtgaacggct gtccttgcga tccagtccag
    24841 gtagatcgag tcgtccacct gcggggtctc gacgatccgt atgccgagtt tcccctccca
    24901 gtcctccttg agcacggcca cgatcttctg gtcgacggcg ttcgtggacg ctttgccggc
    24961 gaggggaatg gagatctcga cacggtttcc atcaggatcg aggaggaccg gtctcccgtt
    25021 tgcctcggtc aggttgaaac cggccgcgac gagctgcctc ttcgcctcct cgatgtcgta
    25081 ggagaaccgg ccgttcgtgg cggggttgat ctggtcgccg gccagggccg ggaggaggaa
    25141 ggccgtctcc gtcgggcggg ctccatcgcc gaccagccag cagacccgtt cccggttcac
    25201 ggcgtgactc agggcgcggc ggaatgcggt gatgtttgcc ggatagaagt cggacgtgaa
    25261 cgccacctcg aaggcctgtc cgcccgaggg gatctgggtg atcccgatgc cgggggcgcc
    25321 cttgagggcc tcggccttcc tcggggagag cccgaactcc ccgctgatga agtcgatatc
    25381 ccctcgccgg agggcgagga tcgccgcgtc ttcgtcttcg tagttccgga tcacgatctc
    25441 gcgcacataa ggttgctcac cgtggtagcc ctcgtaggcg gtcaccttcg agagggtgcc
    25501 cgggatcgtc gacgtatagg cgaaggggcc gatgccgatc tgctttgagt cgacgtatgt
    25561 cgagggtaga tcgatatcct tccagatatg cgcgggaaac accccgatcc ccggaccggt
    25621 cgcaagggtc tcggggaaga gcggtgcgca ggacttcatg cggaagacga cggtgtaggg
    25681 gtcggggcat tcgaccgatg cgacgtttga gaggatcgcg gagttcacca ggaacgagcc
    25741 ggccggagcg tcggcatccc cgtggtcacg gaagtactcg taggtgaact gcacgtctgc
    25801 cgaggtgaag ggggtgccgt cgtgccacgt ggcgttcttc accaggtaaa acgtccagga
    25861 catggcatcg tcggacatct cccacctctc cgcaagccag ccgaggtatc ctccggagcc
    25921 gtcgtcctgc gggcgcagtt tggtcacgag cccttcgtgg atgagggagt cggcgggaac
    25981 ccctttctgg tcgatataac tcgtcgacgg gatgaggtag gtctccccca ggacggcggc
    26041 gaccagctgc ccgtaaggtt gatgcaggtg gttgtgggcg gcgagcgaga gctcttccgc
    26101 agtgagggcc ccggtaccgc ccaggtagcc caggatcgcc gtcgcgagtt cgtctgccga
    26161 gatattggtg tctccgtccg tatcccccgg tatgcctgcc gtcgccggca tgcagcagac
    26221 gaggaggagg acgcagcacg cgagaatttt tgagagacaa cgtgtcgtca tgttcatcac
    26281 cgtgattgat cgtcgtccgg ctcatgccgg aggtaccgga acgatacgcc gagtacggcg
    26341 aggagtgccg caacgcttcc gaggatgggc gagagcgacg caggagtttc accgccttcc
    26401 accggttcgc gtgtggcggc ggaatggttt gcggccgggt cttccttgct atagatccgg
    26461 acttcgtcga cccacttggg ggaggcgccg gcagcggagg gataggatcc gccgtagttc
    26521 cagtaggcct tcccccagca ctcccgcatg tcgttgttcc cgaagatatg ccagccccag
    26581 gggttcgtgg agtcgtcggc aaagaagatc aggcgcatcc cgaccgtgta gccggtgtcc
    26641 gggtagccct ggccctgcgc ctcctgcgcg tcggagcccg agccgttgcc gctgtaccag
    26701 cagaggaccg ccgggccctg ccgcgagtcg ggctcgtaga cgatcgagta ggggaaggtc
    26761 tttgagaatc cgtcccggct gtggagattg atctcgtcgc cctccgaggc accgccgacg
    26821 gagtcgcaga tgtcggcgag gtcggtcccc ttgaccgctc cgaagtcggc cttgaccagc
    26881 atgctcatca gaacatcctc gcccgggttc cagccgtcgt agggctcatc ggggtggact
    26941 tcatcccaga taccctcaaa gacggggccc tggtggtagt accgggtgac gccgtcaccc
    27001 tggacgggca ggttcgcctc cagccagcgg tagtcgacgc tcgtctcgtt taagacggtc
    27061 gtcctgtcgt ttgcgtattt cacgatgcgg agctcggtag tccccgcccc tgcagccagc
    27121 gccggcgccg gcagcagggc gagcatcagc accaggaaga cgattattac ctgatcgggt
    27181 cgtatcattt ggctcaactc acggttcggc gattctggac gcgccttata atatacgttg
    27241 attgggtcga ttccgagtaa ataactttct atttacttat aaaaaatata aatctggtta
    27301 atttaattta gtggtattta acttctggtg aagcgaatcc gcgatgcatc gtgcaaaaga
    27361 cccggggtgt tatacctccg gaacctccat ccgggcgatc tggtacggtg cgatcagcac
    27421 atcttcggtc actgtcctgt tcacttccgt cactttcacc gggtaactgc cgatgggaag
    27481 atagtggaag gtgtggggcg tcttttcgcc ggtgtcccgg ccgaagaggt agatcctggc
    27541 gtccgctggc gtgctcgata ccgagagcga tccgtaggta tagggttcga ggatgaactt
    27601 cgccgtggcg tcgccggagc cgaccgtgag caggatctcc cgttcctccg gaatataccc
    27661 cggctttgat accgagacca ggtgttttcc gccggaaacg ttgtcgacga gatacggcgt
    27721 ggcaaacccg gtctggaacc cgtcgatcgc gatcgccgct cccgccggcg tcgacgtcac
    27781 gaggaggctg cagggtgcgg cggagaacat ggcgttcacg gtatcgcccg acccggcaac
    27841 ggtgggtatc cggtacgtcc ggtaactcat gccgtcgcgg acggcaagat acgcctcccc
    27901 gatcccgctg acctcgacgg tcttcgggaa ccggtcgccc aggtacctgc cgttgaccga
    27961 gaacggctgc tttgcgtact cctccgactc gaatgcgatc gaccgggtca actccgcgtt
    28021 gccgccggta aacatcatcc gtgccacggt gtttctctcg atccagacct gctctccccc
    28081 ggagctgaac gcaaccgcct cttcctgtac cctgatagtg tgcagcccct ccctgagccc
    28141 gtagatgacg gcgggggtct tccggttcac cttgcgaccg tcgacgaaga tctccgcccc
    28201 cgccgggaac gagtcgacgt agacgccgcc gtagtggttc tgctcccggc cgcggagcag
    28261 gcccggggag gccgagcgga ctgaggcgga taccgggatc tcgatcacga cctcgtcgtc
    28321 gtgcgcaagc cggatctccc gctccaccgg ctcggcgccg tccagacgca cccggacggt
    28381 atgggtaccg ccggggagcg acgcgaaggc ggccggggtt gtcctgccgg tatactcacc
    28441 gtcgaggtcg atgagcgcac ccggcggatc ggagagtacg gaaagcccaa agtgcatcgc
    28501 ggtgaggtcg ataggcgtgg gttcaggcgc agcctgaacc agggcggggg tgggtgcggt
    28561 cggaaccggt tcgggctccg tcaccgggtc cgctgcagcg ggaaggagcc cgaacaaccc
    28621 gaggaacgcc tggacggcgc cgagcagccg accgggcaga cccccgacgg tggaaacccc
    28681 tgtataccca aagatcgaga caagcggatt ttccggggtc ttgccggccg gcgccggggt
    28741 acccgatacc gccggcgtcg ccgtcccggt gggggtggtc gcgaggggag tcggggtagg
    28801 ggcaggggcg gtggtggcgg cggtggcagg tttgatctgt atcccggata ccgcaaagag
    28861 ggagaggccg ggagagacgg cctcataggt gttggtctcg ttctcggtac cgatccactg
    28921 tgtctcaagg ggggcccagg tttcgttgta gcggtacagg gtgagatcgt cgcccacgat
    28981 ccagttctcc tgcaaccagg catccggaac gttgaaacgg ataacggccc tgtcgacggc
    29041 gacggtcccg ttccttgagg tgatgttgta gtagcggtag accgcgccgg gcggctcggg
    29101 gatcccgtcg ggtagcagga tttcttcaca ggaaaggttc aggttctgcg ggatctccac
    29161 ggcgaagacg acgatctcgt cgatgcccgg cacctcgaag aggaaggtgc ggttatcgga
    29221 gccgggggat tcggtgtccg cggtatcgcc gccgtcgctg ccggacgtcg ccgtaatcga
    29281 gagttccgcc gtgtcgcttc ccgccacgtt cgtgaccgtc agggtgaccg tgtagatgcc
    29341 gacggcggtg taggtgtggg tggggttctg ctcgtcggag gtcgtgtcgt cgccgaagtc
    29401 ccagaaccag gaggtcgggg tcggaacccc ggtactccga tcggtaaacg ccacggcgag
    29461 cgggaccgtg cccgaggtca cgttggcgcc gaagtctggg gtcggcggtt ccggctcctc
    29521 ctggctgtag acccggacct cgttgaccca cttgccggag gtgccggcgg cagaccagta
    29581 atccccgccc ttgttccagt actcctcatc ccagcactcc ttcatgtcgt tgttcccgaa
    29641 gatgtgccag ccccaggggt tcgtggtggt gtcggcaaag aagaccatgc gcatgccgac
    29701 gtcatacccg gtgtccgggt agccctggcc ctgcgcctcc tgggcgtcgg ggcctgatga
    29761 gttgccgctg aaccagcaga ggaccgccgg gccctggcgc gggtcgggat cgtagatgta
    29821 ctcgtagggg tagcgcttgc tctgccaccc gtcccggctg cggacggcga tctcgtcgcc
    29881 ctccgcggcg ccgccgacaa gttcgcagat gtcggcgatg tccgtcccca tcaccgcccc
    29941 gaggtcggcc ttgaccagca tgctcatcag gacgtcctcg gccgggttcc agccgtcgta
    30001 ttcctcgtcg gggtgggcat cctcccagtg cccttcgaag acggggccct ggtggtagta
    30061 acgggtgacg ccgtccccct ggaccggaag gttcgctttg agccagtggt agtcgacggt
    30121 cgtctcgttt aagacggtcg tcccgtcgac tgcgtacttt gcgatgcgca cgtcggtggt
    30181 gcccgctgcc agtgccggtg ccacgagcag tgcgacaacc agaataaaca ctggcattcc
    30241 gattgtccat cttttcggtc tcgtcataag atcatcccgt ttgtgccgga agatactcca
    30301 tcggatacgt cgttacattc gcatgcgtga gatgcaggat atccttcgcc gatctccctc
    30361 gccggaaaaa gggtagaggc cccattcagc gcctcttcca gaggaggtac ccggcgagcg
    30421 ccacaacgat gacgaccgcc agggcgatcc agacgagcgg gctctcgccg gtctccgccg
    30481 ggggaacagg tggggtcgtg gcggcggcgg cgggcaccgt cggggtcggt tcggtcggga
    30541 ccggcgcttc ggtcgcggtt ccgacgaaga gggcgtaagt gctgaagtgg ctgaccgatg
    30601 cggtcaccgt tcgggcgtcg gcatcgacgt tcgtcgggca ggcggtccac gtgccggtcg
    30661 catcgtcgta gccctgcacg accagccggg ccgaatcgcc gcctgttttg ccgagtaatg
    30721 cggcccactc atccccggtg aaggtgaaga caaggtcgat cgccggagag aaggtggcac
    30781 cggcggggga gcagtcgacc gcgtagcccg agaaggcgag ggaagacgcg ccggggaggg
    30841 tcgagacggg ggcgatcccg acaccggaga gcgggcttcc cgcgtcgtcg agcgccgtaa
    30901 ccccggtctc cagggagagg tgcgcgaact tcccgtcgac gtagacgagg tagggccgga
    30961 gcacgccgcc ccacgaggcg gtgaggaggt cggccgtgcc ggtcgaggtg accgttgccg
    31021 tgctgcttcc gctgccggag gtccggggct gcgtgggtgt cgatgtcggc tccggggtcg
    31081 tggtcgtagt gacgggtgca aatacactga aggaatccgt ataccccgtt atagtaaacg
    31141 tggtgtcgtc ccttgtctgg ttctcaagtt cgatccagtt ttgaccatcg taccgctgga
    31201 gctttgtggt tctcccgtca ccgaacagct cctcccattc gtcctgggtg aaggtgatgg
    31261 tgatcgggat cttcgggttg aacttcgcac cctcgggccc gagcttgaat gctttgcctc
    31321 cgatctcgat ggtccccgca gggggctctt cgagatctgg ggcaaccccg acgctgaatt
    31381 ccgttattgg gctgccgtcg gcctgcttcg cgtcatgacc cttcgggatc tccagcgtga
    31441 ggccttgata cgttactgaa atatcttcgg ttgccgtcgc attggtctgg tcgaccctgt
    31501 cggtcacgtc ttctttttga gggggtgcaa caacggtgat cgccgttgtc ttcacgtcgc
    31561 tgccgccgct gttcgtgacg gtcagggtga ccgtgtaggt tcttgcccct tcgccgtcat
    31621 gatcgaatac gtgggtcgga ttccggagag tcgagttcgt accgtcgccg aagtcccatg
//
LOCUS       DAOSEE010000006        29728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_746_length_29728_cov_272.127827, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000006 DAOSEE010000000
VERSION     DAOSEE010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 29728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29728
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_746_length_29728_cov_272.127827"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..1220)
                     /locus_tag="PLI76_01065"
     CDS             complement(210..1220)
                     /locus_tag="PLI76_01065"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOI60649.1"
                     /translation="MGYSIQELSEENAGDWDEFNNRCPEGTFYHTVKWKNLLENTLNI
                     DLKYWIIWKGQKMVGILPFMNRPLVFLKGLEPIPHSEFNNILLKDDLDLRDLDKVLTL
                     FASKYSFLSLNMDSKLLWDHMGYNHHSSENTGNMVLDLRQNPPDKIWEDFTARKGQRR
                     FIRRFGEKGFWIREIHEQEDMKTFYRYYAENLARINGDILPLAFFQSLLEAYSQNGMR
                     VTLLSKEDICAGGLLTFVHPASKTVYFEYLSLNRGLPNTYHPTYPLFWEGLNWAWDNG
                     YQKVSFGRQRFDPKSARFQIKAGFGAKYVPIYSNIVLFSKTASMFYRFKRIYRGRSAS
                     AG"
     gene            1696..2265
                     /locus_tag="PLI76_01070"
     CDS             1696..2265
                     /locus_tag="PLI76_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60650.1"
                     /translation="MEEKHIIPCTFMAREVCEFTRPSYGYLVQRTWNDSIKAPLIPII
                     KRIPYTVSLWRQIQLMISPADDGTRDTNQLKMAQRGVMSVDEVLDLQPGELVQVRSLD
                     EIYSTLDGEGKYKGLLFMPEMEDFCEKKYHVFKKVRSITLETDGEVRKLKSPTVFLEG
                     VYCDGKRHNNCDRSCLLFWREAWLKRVEP"
     gene            complement(2867..4333)
                     /locus_tag="PLI76_01075"
     CDS             complement(2867..4333)
                     /locus_tag="PLI76_01075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOI60651.1"
                     /translation="MKRADCTHLGFRMPGFGIAPQFPRHVPSLAIRQLPVDLLDAAME
                     GKVEVPGAGYLGDNRRIPDIVRYKSERGTSSPGAGMNVIILDVHPVEDVRIERHITYL
                     ADQGMNVCRIHYNYADESAGPGIFSHHGVRGFRINRFFFQGKTRTLYFMGYCLRPKIL
                     ADCRKALKALDIDPEQPSILHIHDPLLLPLAGKLVENSMPNSRVIYDRHEVYEDWLHF
                     GRISLPLFYEDLAKKWISGTVVVSEHHRDAVHRLFPGSHIIAVPNYAASAMYDANAID
                     EKIRLAGPEPQIHAVYIGSLNNAADRDVDLLLDIADAVIRSYDNVTFFIGGTSMDEGS
                     RVKIEELAGRHDGRLRFLGYVPREKTVKLTERAHIGFLLVRPDARYWVRTSPNKIFEY
                     LVCGTVPIIRADVDHADRLRACSLIFDREDEDAMIVQAVLDLLGSPEKLKRYMGKARA
                     LGLDYTWESVACRYIDLYTMLLYPETAPSHPNALSPMK"
     gene            complement(4330..5448)
                     /locus_tag="PLI76_01080"
     CDS             complement(4330..5448)
                     /locus_tag="PLI76_01080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOI60652.1"
                     /translation="MRLLVCTTEYFPHGAGMANVVYNVVQQLKGRGVECTVCSPKGPD
                     VRLGSWPLIERAGVVGLLNYWYQVSRHFKNNDYDAAWLQNPFIFTKNPFKRCLVTMHS
                     TYSGSSELGVGSLPFHLYESVVARIERSCLTRMPSTTLFTGVGRPVREELERIGVARD
                     RISYIPNGVDIGRFHPSPDRTGIRKRFGLPEDGVILLSVGRLIPAKQPLTMLEIFSHL
                     EKQRKGVTLCIAGGGELLEAAMNLVRRMGLENVLFLGNVDHGRDLPDLYACADYYMMV
                     SKYEGGMPPLTLAEAMASGLPCIVSDIANLRVVKEADCGVVVAPDDPATAAAAILGYL
                     NEEHPDHAMNARRYAEKVLNWESISERYVNMFECLTTG"
     gene            complement(5567..6718)
                     /locus_tag="PLI76_01085"
     CDS             complement(5567..6718)
                     /locus_tag="PLI76_01085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOI60653.1"
                     /translation="MRIAQITPYFLPHNGGMEMYVYNLSKGLINEGHTVEVITSNIPE
                     GRTFEVMDGISVRRLRCIGEPLRNPLVPSIFLLINELKNFDIIHIHNVYSFVALSFPL
                     LKRLCKVPLVLTHHGQLMFGEHIGDTCVRIYEKSARRVILNSVDRTVTLSKSDARHIA
                     QYNGASEDHGISLPLRRVVTIPNGYDNTKFYPMNTGECREALGLPTDKKVILNVGRLY
                     GVVKGHEHLINAMSRIVRERTDILCVVVGPGRLYDALKSQIGSLGLEDHVMLAGGKPH
                     DEIPLWLNACDLFVLPSLSEGNPTVMFEAFGCGKPFVGTRVGGVPEVVTSDKYGLLVD
                     PGNPDDLAEKTLIALDREWDSEAILAYSERYTWKTIAKEIVGVYGQVLG"
     gene            complement(7518..9467)
                     /locus_tag="PLI76_01090"
     CDS             complement(7518..9467)
                     /locus_tag="PLI76_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60654.1"
                     /translation="MEAEFGLRVQLFRRLTGIFPYLVLFTYVAGCAVCLLIGKMSYLV
                     NGSILAVPAIIGSFAFLLVKKKDIELSGVLEVYSYNRLISLRLFLTIFTLTLLGFLIT
                     PPESGLGLVGVLILYAVIFTQILSARLRPVMVLIEIMLTLAVTIYSYTLRPALYFGTT
                     DLMPHSYMATITYVSGHIIPGELGTYTYFPLYHVFVALSSHVIGLNIETSLFITTGLI
                     FTTTVLFLYYLIKRIFQSDQIALLIVLVYAMNADVIYYGTYMVTRTMAYVGFLILLYL
                     VYSIVETRPEAEYAVTGSTTRRAFAVIVALFILLIHQISMPMIIALIGLLYLFERLTN
                     ERRRVSITFLMVPVSLFLSYWLFIAYPFIEDLLPRIDSSIYQNLALTEAVHQGWGFLL
                     NQVDTLFIVFFALLGAIYLLWKQRPEYSVVFGILGMVAIILNVPGIFTVMFQMVSVLR
                     IERFAILFLPILTVAMGVGIYILMRYLVALGLSPRRVGAVMIVLIALYGISALGFIRE
                     DPGYIRYSFNQAEVIGFHHILEKVPSGSLLHTDYYTLRFFGRKKISESEHLRLPYYTG
                     RLIPDDPNCSWGSGYVILLEAQLRGGGLLFGDEEAMDETKFDPGKGLQPYLPTEGNVL
                     NLTGRLSTEDRIYSNSGISIYHLQQ"
     gene            complement(9738..11210)
                     /locus_tag="PLI76_01095"
     CDS             complement(9738..11210)
                     /locus_tag="PLI76_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60655.1"
                     /translation="MVSTEYKQAFGAGANPTGSPIGGGDGYKDILTPTQPGAGNIVTT
                     AGELFDALRDAESGDIIYIDETARINLTGAPAGVVIPPGVTLAGNRGERSVTGSATYA
                     FEIKEPGDYAFRVRASGLNGGGGTIRVSVDDEEIRRWDLDEVGQDWHWSTGEVRYLSG
                     GKHTLSIYWREDDPSLDAVLITGDIGYLPDTAAGGQSGRDNIHVEAESGDLSQGLEKI
                     ADPAASNGAYISAQRNSTARGEYPVSPGGLIYLRAADSDPRIGFIAGGENVRITGIRL
                     EGPDTGNETDVPSALGIHSAYRNLEVDNCEIWGWNGAAIGIAGTGGSDMKAGGYIHHN
                     YIHHCQMEGLGYGVVVSGGATSLIEANYFDYCRHAIAGSGVAGDGYEARYNICGPNFI
                     VSSSHNFDMHGVVTGSTTIAGDTIKIHHNTFMATGPLSSFPVAIRGVPRDGAYIDHNW
                     FYYTQAPPVWQTGGQERVFVTENLIGQNGIFSESGPISYY"
     gene            complement(11624..13429)
                     /locus_tag="PLI76_01100"
     CDS             complement(11624..13429)
                     /locus_tag="PLI76_01100"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HOI60656.1"
                     /translation="MKKPLFLALALCMAIAMVTPALAVQSQEVSQGTYDIVVYTNGAK
                     IVAEDSNGRVISSGTAGIDDSTVIQSAAKAISNGIVLLQSGTYVLTEPAGIRVSNPTP
                     TPTPTLTPTPTPVPSGKPDLVVTDISWEPANPMTGDTITMKAVIKNQGTGATPAGTIH
                     GVAFTADGNLGSAVWSDTYIASIAPGAWVLVTANGGFSGSTWKAVAGTYTITATVDDV
                     NRIAESNENNNALKKTMTVKAPTSTPTPTPTVTPTPTPTKTPTPTPTVTPTPTPGQNV
                     YGADANPTGNPIGGGNGYKSTVSRSDADFIVDTTAKLVSALSSAQSGDVIWVEGNANI
                     DMSGRTTTIRSGVTLASDRGQGGSDGGRIYQTSSGTRLFTIGGQNVKITGLRLEGPQK
                     STSASGSTNVGIYTSYRNLEVENCEIFGWGNAAIGIAGTGGSDMKTGAYIHHNYIHHN
                     QVAGLGYGVCLSSGGVALIEANYFDYCRHGITGAGNAGDGYEARYNICGPNWISTSPH
                     NFDMHGVVTGSTTIAGDTIKIHHNTFLGTTSQMPTCIAIRGVPRVGAYIDHNWFYFTR
                     DAPVWQTGGKNGISMTNNLIGPSGTLSASGPIKYY"
     gene            complement(14014..15561)
                     /locus_tag="PLI76_01105"
     CDS             complement(14014..15561)
                     /locus_tag="PLI76_01105"
                     /EC_number="2.4.2.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ribofuranosylaminobenzene 5'-phosphate
                     synthase"
                     /protein_id="HOI60657.1"
                     /translation="MEPLTADRISAKIHEIEQRVGKLSPMQKVLIGTDGSVTNLLEMA
                     TGHSVSITTRVQDIVAADTEAAAALAIEPGDEVNHRIVELKDSVTGNVLIYAVSRTPL
                     RRLAPEFRQDLMRADIPIGRILSRHRIESRREITDARVVQAGTDLARTFNIHRCESML
                     SRKYRIIHRGEPLIAIEEIFPCTAFADDVRVLVDAPSRLHITLLDMNGTSGRVDGGVG
                     VALDEPGCLLDARKGPGLDVHGGDELLRNRVLEAARAVKEGLGLPGGADITVHAVARP
                     HAGLGSGTQVALATAAALCRLYDRNVPVPDLARIVGRGGTSGIGTAAFAQGGFILDGG
                     HRFGSPGGKQDFRPSAASRGVAPPPVLARHRFPEDWRILLVTPDIDAGAHGRREIDIF
                     RTHCPVPLHEVRELCHEVLMRMIPGLVEQDIDLFGSAINRTQVLGFKKVEVAMQHPVV
                     SSLIEAMVEAGAAGAGLSSFGPTVYAVGDSGMADVARAAKEVLGDRGGTVIPTRARND
                     GAAVRAE"
     gene            complement(15626..16780)
                     /locus_tag="PLI76_01110"
     CDS             complement(15626..16780)
                     /locus_tag="PLI76_01110"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HOI60658.1"
                     /translation="MKYILILGDGMADEPLAELGGRTPLEYADIPNMDRIAREGRCGM
                     LRTVPDGFEPGSDTANLSVLGYDPRTSYTGRGPLEAASMGVDLRKGEMAYRCNLVTIR
                     DGVMEDFNAGHISSTEGAGLLRDLDAAFEDVRVYPGVGYRNLMVVPGARGAVTTPPHD
                     IVGQAVRDYLPRGGDAAILLDCMERSREVFAGHPVNRRRLHEGKAPATGIWPWSGGEK
                     PSLAPFREKYGLSGGMISAVDLLNGIARLAGMEVIHVPGATGFLDTDYEAKARYAVDA
                     LDRLDFVYMHVEAPDEAGHMGSVEEKVRAIERLDEAIGIILDRPETAVAVLPDHPTPI
                     RLKTHTADPVPFAMLGEGRDGVRAFSERDAAKGSFGLVQAPDFLSLFFSC"
     gene            complement(16892..17839)
                     /locus_tag="PLI76_01115"
     CDS             complement(16892..17839)
                     /locus_tag="PLI76_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOI60659.1"
                     /translation="MFCIVTGGAGFIGSHIVDALVAEGNDVLVIDDCSAGTEENLARH
                     AAGGRVAFIRQNLLDDGWQEHFAGADRVYHIAADPDVRQSAVTPDSQVRNNVIATHRV
                     LEAMRAHDVPELVFTSTSTVYGEATVIPTPETYTPLEPISVYGATKLACEALISSYCH
                     SFGMRAWVYRFANIIGERSGHGVISDFIRKLRENPGELEILGDGRQTKSYLEVGECVR
                     AVQFGIDHSHDPANTFNIGSEDWIDVVTIADIVAEEMGLSGVRYRFTGGARGWVGDVP
                     KMQLSVEKLKALGWRCGTTSEESVRTAVRAMLGKEGARP"
     gene            18233..19108
                     /locus_tag="PLI76_01120"
     CDS             18233..19108
                     /locus_tag="PLI76_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HOI60660.1"
                     /translation="MSNPRPDIRGHAGIYLRGLMMGACDIIPGVSGGTIALITGIYER
                     LVGAIGSIDPASLKHLARGDFASFRRDLESLDIPFLVVLLAGIGTSFLLMSGIILSLL
                     TDHAVATYSFFLGLILASAVLIFLEIRSPGVATLAYLAIGTGAGYLLGGLGNFDVGHS
                     LPVLFLTGMVALCAMILPGISGAYMTLVLNQYEFMLAALRALSLPEIVAYIAGGVTGL
                     LLFTRGLKYLLKTHPEAMLALLTGLMLGSARMLWEKGSLAGNMVAGGSVFFIAGLVVV
                     GAVEYLKRRYAGSGA"
     gene            19174..20046
                     /locus_tag="PLI76_01125"
     CDS             19174..20046
                     /locus_tag="PLI76_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 12 protein"
                     /protein_id="HOI60661.1"
                     /translation="MFIGIDHGTTAMRFSTGMQEFKISREEARDFSASDLSRLSPLEE
                     IEGIAVCYSMGDGISAITDIRKVPDRGIVSREGAGKHIGGGTRVYDAIKESGLPAVVI
                     PGLHRGSPTDPRFKAYSHQASPEKIGILYEVVHDLGGDVVVCDASSNTVTLLVTGGRI
                     TGAFDACIFAPGTRHGALDVDAIRRIDRGESTANDAFLHAGVDNTMPPDLRVETMAMF
                     AAMECAAMLLINPGARVALAGSMAPAIAPGVKTLLARDVAVYDEWCAARGLARIARDV
                     FSGATGVLGLMVDL"
     gene            20266..20433
                     /locus_tag="PLI76_01130"
     CDS             20266..20433
                     /locus_tag="PLI76_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60662.1"
                     /translation="MEQQFAGFKVGERRKMIDRREFTNLDGEVNYVALMNKFDEVGER
                     LLEESRLLRRH"
     gene            20645..21883
                     /locus_tag="PLI76_01135"
     CDS             20645..21883
                     /locus_tag="PLI76_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60663.1"
                     /translation="MLKVALDRASIPQDEKEKVVRIIEQLEDISIDNSISRLSISINN
                     DRLGYVDLTPFMQVCDAFLKPPHWMRPPTYYVTCAKPYIPETTLRYKRGEIDEINCAP
                     YIKVNSVIGVCAQYAMRMALMILSPKAPTVPELISEAINTALSGGVEREPDIGWHPDE
                     IHHFFEISGYGVFRYSRSQLRCDNCGHLVSDMRMVPGIENIYAYVESGLPVIIGVRNT
                     KYLPWWGDGDEAHALVAIGHTLDGNGRVDGIIVHDESTYPYQILKEPLAGGKTLEDVI
                     FEVIAPVPREVMVEYQVARSLATQFFDLDEEDIIRPVLIDSNQIKKWLGEGTRRRHLE
                     AYDLPAVVKEDFLSAYMDRYVWLFEMRKERGDKREYVGDVIVGAINPSILGFVMPEQR
                     IYGFRDGSKGPHAKHFDDAV"
     gene            21917..22057
                     /locus_tag="PLI76_01140"
                     /pseudo
     CDS             21917..22057
                     /locus_tag="PLI76_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843549.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 12 protein"
     gene            22162..22779
                     /locus_tag="PLI76_01145"
     CDS             22162..22779
                     /locus_tag="PLI76_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOI60664.1"
                     /translation="MQLECPSIADLMLQMASSLEDTARTMDQGNANRFLDEILNAKRI
                     YLAGAGRSGLVARAFAQRLMHLGFESYVIGETITPAFGREDVLIAFSGSGETRSVVDA
                     CETAREIGGKICLITSTPDSRIGHMADCIVEIGNNRFKDANIPSDFEIRQLTGQYRSV
                     SSSFAPLGTLFETAALVFSDAIISALMEVKHCTVEELRDRLANVQ"
     gene            23184..25265
                     /locus_tag="PLI76_01150"
     CDS             23184..25265
                     /locus_tag="PLI76_01150"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOI60665.1"
                     /translation="MTRTANLFLVILILTVALLPVSAMAEAGTTELRIVRYASDNRTV
                     LDETTVDYLWMKANLPVYGDGRTHYFHEGPVLEEYWENAHPGEEYDPWDPAEDVQGSV
                     LQKGDLGAVMGTGVTDLCALIGGAEEGDRIAVTCRDGWRKTFPYEYIYRPDPRQGPLV
                     ICWFNGDEAPGEKQGVGYPDTTYLSGMRMAFFADTSSNPWGWHIFGNNDMRECWDEEY
                     WNYGAQYPSAVGVSGKWVSEIGIFTDRLPPVPVAEFETNVTSGRLPLAVAFNDTSTAN
                     PRTWTWNFGDGSSSAEKNPVHVYTTAGDYTVTLTVSNVAGTASVIRAGAVTVENAVVP
                     TADFAANATSGEAPLAVAFTDASDGKPAEWEWDFGDGEGATGPAPVHVYSSPGTYTVA
                     LTATNHKGSDTITKTGAITVDVPVPAPPIASFSTDVRAGDAPFEAAFTDTSQKFPTTW
                     AWDFGDGASSEEPNPVHIYSSPGVYTVALTVTNDLGSDMVTRPQFIAATENGKAILFR
                     GTVPLGEGTFSVKAPSGQAYTPNKRTPMGALDAVAEMRGFTYVIGDKKYSESRLLFLD
                     GVGEYLLEKSTGKTWVCFHNGELLDDYGRPSTDAFNRRPVADGDTVLFWYGDYFIYST
                     GKMEPRGGGVCLDNTLAIVEMTIASEPAAGGATAAQAGAASTSAPAPESPPGVWLPIL
                     AIGLFLVLRRV"
     gene            complement(25442..25714)
                     /locus_tag="PLI76_01155"
     CDS             complement(25442..25714)
                     /locus_tag="PLI76_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOI60666.1"
                     /translation="MTWRLLLMPRAEQKLNKIPDHDATRIKDELRALANEPYPHLYAK
                     KLKGHPNSPLYSFRVGQYRIILVFEGNVMIITVIDIGNRSNVYRKY"
     gene            complement(25714..25944)
                     /locus_tag="PLI76_01160"
     CDS             complement(25714..25944)
                     /locus_tag="PLI76_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60667.1"
                     /translation="MATTIQLQPETKSRLDTLKTHPRESYDETLNRIMDAFFDPEPLS
                     EEILQEIEESIADMRAGIRGRPFEDYVRERGI"
     gene            26652..27344
                     /locus_tag="PLI76_01165"
     CDS             26652..27344
                     /locus_tag="PLI76_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOI60668.1"
                     /translation="MKLITVAGPPSSGKTSVILKTIAALGLPEGSVGVVKFDSLTSFD
                     HQRYDEQQIPNLTAFAGKICPDHFFVSNVERAVEWGTKKGLSVLITESAGLCNRCSPY
                     VNAILAVCVIDNLSGVNTPRKIGPMLKYADIVVVTKGDIVSQAEREVFAFNIREVNAS
                     ATVLFVNGITGQGAFMLARYCLDALDIQTLRDRKLRFTMPAAICSYCTGETLIGEMYQ
                     MGMVKRMEFDDA"
     gene            27341..28375
                     /locus_tag="PLI76_01170"
     CDS             27341..28375
                     /locus_tag="PLI76_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI60669.1"
                     /translation="MKALDYVAEVSAVPIGDILRRYPLAVDYLANVRLSGIDGTKTLP
                     EILEEVDEEMLEEFGLDREEVIFHFCAFLESFSRTKGTDEAISSITILGGRDKAGRPE
                     MVELTITPGEVVSIVGPTGSGKSRLLEDIECLAQRDTPTARQILINGSVPDLNKRFST
                     GGKLVAQLSQNMNFVMDLTVQEFLEMHAKSRMIAGSAAVVEKCFAVANSLAGEKFTLD
                     TKVTQLSGGQSRSLMIADTALLSSSPIILIDELENAGIDRREAIEILVSNEKIVLMST
                     HDPLLALRADKRIVIKNGGITKVIETTAEEEQTLQQIEAIDSILLTIRNQLRGGGVIA
                     KASIPAFQEG"
     gene            28379..29143
                     /locus_tag="PLI76_01175"
     CDS             28379..29143
                     /locus_tag="PLI76_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HOI60670.1"
                     /translation="MWELYDALIEEIPDDIIVDDVVVGGEMTYVGANGNVGLAAYRGY
                     VQRAPMMTGNRIGKSLKEVAGCVKSWNLLEASIGNSAINAWYNHPDVAKRAGIEVPEK
                     KRVEERLNDPFIKSQNLVKGKKVCVVGHFPFLEKLIAPVCDLSVVEWDPEEGDYPYSA
                     CEYLLPECDYAFLTCAAVGDKSMPRLLELSENADAVTIVGPGTPLSSVFFDYGVSDLS
                     GFVVTDVMLAKRIVAGAESQRIFGAGMKVQYLRPGV"
BASE COUNT         6173 a         8545 c         8623 g         6387 t
ORIGIN      
        1 tcacggctat ggttatcata ctcttccttt ctggtaaaca actcccagat ctcgttatcc
       61 tgcttgagca ggtctattct ctgcaattat cttctccgca tacttagagc aggggatatt
      121 atgcttttcc ccgcctcatt tcccggaaga gacaggatag aatagaatat cttatcgccg
      181 cactcccggt tcggccatcg atctctatct cagcccgccg atgctgatct cccacggtat
      241 attcttttga accgatagaa catcgaggct gttttcgaaa acagaacgat atttgagtag
      301 ataggcacat attttgcccc aaaaccagct tttatctgaa aacgggcgct tttggggtca
      361 aatctttgcc ggccaaagga gaccttctga tatccgttat cccacgccca gttaagcccc
      421 tcccagaaga gaggataggt aggatggtac gtattgggga gacctcggtt cagggaaaga
      481 tattcgaagt ataccgtttt gctggcagga tggacaaagg tgagcagtcc tccggcgcat
      541 atatcctctt tcgataacaa cgtcacccgc atcccattct gtgaatatgc ttctagcaga
      601 ctctgaaaga aggcaagcgg caggatatct ccgtttatgc gtgcgaggtt ttcagcataa
      661 tacctgtaaa atgttttcat gtcctcctgc tcgtggatct cgcgaatcca aaaccccttc
      721 tcaccgaacc tgcggataaa tctcctctgc ccttttctgg cggtgaaatc ttcccagatc
      781 ttatccggcg gattttgccg taaatccagc accatattcc ctgtattctc agaggaatgg
      841 tggttgtatc ccatgtggtc ccataataac ttactatcca tattcaggga gagaaatgag
      901 tactttgatg cgaacagggt gagaacctta tccaggtccc ggagatcgag atcatccttc
      961 agcaagatgt tattgaactc tgaatgaggt ataggctcaa gccctttcag gaatactaat
     1021 gggcgattca tgaaggggag tatcccgacc attttctgcc ctttccagat gatccagtac
     1081 ttgagatcga tgttgagtgt attttcgagc aggtttttcc attttacagt atggtaaaac
     1141 gttccttccg ggcaccggtt gttaaattcg tcccaatctc ctgcattctc ctccgacagc
     1201 tcttgtatgg agtagcccat gatggcttac tactttcccc tgatgtaatg taacttccgg
     1261 ctcgatacac tgcggttctt atcagtaacg gatggtttta tcctcctttc cgggcaacac
     1321 cagggtcgcc ccgaacaccg gtgaggggca ccgcatggaa ccggggtctg gatctcccca
     1381 catctcccct ctgtgggttg ctgcactatc caggtggata ctacccaggc ggagcgggat
     1441 catgcgatct cttcggtgcc tgcagcaaat ggtttatgta ttatgacatc ctgaacccgg
     1501 cctgtccagg gtctctaccg gtttgcccga tcgggtgaga gggaaagagt gtggccgacc
     1561 ctataccttt cccgggacac tgcatgaaac gtcccggcag agctcctgat aattacagga
     1621 ggaacattgg aatgcgcgaa tgccggtcag aggtgtatga gatccggttt agtggtggat
     1681 aagggagagg gaagtatgga ggagaagcac ataataccct gtacattcat ggcccgggag
     1741 gtatgtgaat ttacgcgacc gtcgtatgga tacctcgtgc agaggacctg gaatgattct
     1801 atcaaggccc ctttgatacc tatcatcaag cggatcccct ataccgtatc attgtggcgc
     1861 cagattcagc tgatgatctc gcctgccgat gatggcactc gtgatacgaa tcaactaaaa
     1921 atggctcaaa ggggggttat gtctgtggat gaagtgcttg atctgcaacc cggtgaatta
     1981 gtccaggtcc gatcgttaga tgagatatac tccacgcttg acggagaggg gaaatacaag
     2041 ggcctgctct ttatgccaga gatggaagac ttttgtgaga aaaaatacca tgtgttcaag
     2101 aaggtgcgct caataaccct tgaaaccgat ggggaggtga gaaagttgaa aagtcctacg
     2161 gtcttcctgg agggagtata ctgcgatggg aagaggcaca ataattgtga cagatcctgc
     2221 ctgctcttct ggagggaagc ctggttgaag cgggtcgaac cctaacccac ctcattactc
     2281 ctgcactcgg agcatgtcaa ctaccccgcg ccttttgggt ggggcttgct gccggggccg
     2341 gccccttgcg gggcacaggg cagcaattta atgggtgcga aggttgacgg ttcaaaggtc
     2401 gtccttattc gcacgggccg gttgacgcgg cccctaccga ttatccgtgg gatcacggaa
     2461 tcaccggcta cctttctcag atgattcaga gcgccgttca cgtcggcgtt gatcagggtg
     2521 cccggagcac tctggtagag cccgcgcttg acccgccgtt tccgcccata gggcggtttc
     2581 ttgattggat caagcgcgag agcatcgacc tgcgaggtgt aggcttcaga atgagaatca
     2641 tcgaaccgga tcccggccaa ctcgcacttg ctcttcaacg cactcgcagt gccattatct
     2701 cttatcattg ttgtttatcc gtcatgaaga atagccgcgg ggtgaaagtg tacggggctt
     2761 gtatacggac aagcccggga caggcagacg gcattcctcc ccgcacctgt tggccggggt
     2821 ctcctgccta tacgaagatg aaacgaccag tactgtacct ctcttttcac ttcattgggg
     2881 agagtgcgtt cgggtggctg ggagcggttt ccgggtatag taacatggta tagagatcga
     2941 tataccggca cgccacagat tcccaggtat aatcaagacc cagcgccctg gccttaccca
     3001 tataccgctt cagtttttca gggctgccaa gcagatccag gaccgcctgc acgatcatcg
     3061 catcctcatc ctctcgatcg aagatcagcg agcatgccct caaccgatca gcatgatcga
     3121 catcggccct gatgatgggc accgtaccgc acaccagata ttcgaagatc ttgttgggcg
     3181 aagtcctcac ccagtatctc gcgtccggtc tcacgagcag gaagccaata tgggcccttt
     3241 cggtgagttt gactgtcttc tctcgcggaa catagccaag gaaccggagc ctgccatcat
     3301 gccttccggc aagctcctcg atcttcaccc tcgagccctc gtccatggag gtcccgccga
     3361 tgaagaaggt cacgttgtca tacgagcgaa tgacagcatc ggcgatatca aggagaaggt
     3421 cgacgtctct atcggccgca ttattgaggc tcccaatata caccgcatgt atttgcggct
     3481 caggcccggc aagacggatc ttctcatcta tcgcgttcgc atcatacatc gcggatgccg
     3541 cataattcgg aaccgctatg atatgcgacc cgggaaatag cctgtgaacc gcatcacggt
     3601 ggtgttctga gacgacgacc gttccggata tccacttctt tgcgagatcc tcatagaaga
     3661 gcggcagaga tatccgcccg aagtgaagcc aatcttcata gacctcatgt cgatcgtaga
     3721 taacccgtga gttgggcatg ctgttttcga caagctttcc ggccagcggc agcagcagag
     3781 ggtcgtgaat gtgaagaatg gacggctgtt caggatcaat atcaagtgct tttagcgctt
     3841 tacggcagtc ggccagtatc ttaggccgta aacagtaacc catgaaatac agggttctgg
     3901 tctttccctg gaagaagaac cgatttattc taaaccccct caccccgtga tgggagaaga
     3961 tgccgggtcc ggcggactca tccgcgtaat tataatgtat ccggcagacg ttcatcccct
     4021 gatccgcaag ataggttatg tgcctctcaa tccgtacatc ctccaccgga tgtacgtcaa
     4081 gaataataac attcataccg gcacccggcg atgatgtccc tcgctcagac ttatatctaa
     4141 cgatatcggg tatacgccga ttatccccaa gatatccggc gcccggaacc tccactttcc
     4201 cctccatcgc ggcatcaagg aggtcgaccg ggagctgacg gatcgcaaga gagggtacat
     4261 gccggggaaa ctgtggagcg atgccgaaac ccggcatccg gaaccccaga tgtgtacagt
     4321 ccgccctctt catcccgtgg tcagacactc aaacatattt acgtacctct ctgatatact
     4381 ctcccagttc agtaccttct cagcatatct ccttgcgttc atcgcatgat cgggatgctc
     4441 ctcattcagg taaccaagga tcgcagcggc agccgttgcc ggatcatccg gggcaaccac
     4501 cactccgcaa tcggcttcct tcacaacccg gaggtttgcg atatcggaga cgatgcacgg
     4561 cagccctgag gccattgctt ccgcaagggt caggggcggc atcccccctt catacttcga
     4621 gaccatcatg tagtaatctg cgcaggcgta gagatccggg aggtctcgac catgatcgac
     4681 gttcccgagg aagagtacgt tctccaatcc catcctccgc accaggttca ttgctgcctc
     4741 caggagctct ccccctccgg cgatacagag cgtcacaccc ttccgctgtt tctcaaggtg
     4801 cgagaagatc tcaagcatgg tgagcggttg ttttgcaggg atcagccgcc cgacactcag
     4861 gagaataacc ccatcctccg ggagaccgaa tctcttccgg atacctgttc tgtcgggtga
     4921 gggatggaac cgcccgatat caaccccatt cgggatgtag gatatccggt ctcgtgcgac
     4981 accgatccgc tccagctcct cacgaaccgg ccgcccaaca ccggtgaaga gagtggtgga
     5041 gggcatacgg gtcaggcagg aacgctcgat ccgggcaaca acagattcat acaggtggaa
     5101 aggaaggctc cccaccccga gctcgctcga tccggagtag gtcgagtgca tcgtcaccag
     5161 gcagcgctta aatgggtttt tggtgaagat aaaggggttc tggagccagg cggcatcata
     5221 atcgttgttt ttgaagtgcc gcgagacctg gtaccagtag ttgagcagcc cgacgacgcc
     5281 cgccctctct atcaggggcc agcttccaag cctgacatcc gggccttttg gggagcaaac
     5341 cgtgcactcg acgcctcttc ctttgagttg ctgcacgacg ttgtaaacta cattggccat
     5401 gcccgcgccg tggggaaagt actcggttgt gcagacaagc agcctcatgc aggaattccc
     5461 tccaccatcc agaccggatc gcctgttcgc tccagagcgc gtccggcagc aatcaccctt
     5521 atcctcggat gggataacgc tggagagtcg ggccatcctc tacccatcat cccagtacct
     5581 ggccgtatac gcccacgatc tcttttgcta tggtcttcca cgtataccgc tccgagtatg
     5641 cgagaatcgc ctcgctatcc cattcccgat ccagcgctat gagggttttt tcagccagat
     5701 cgtcagggtt gcccggatcg accagcagac catacttatc ggaggtgacg acctccggga
     5761 ctccgcccac cctcgttccc acgaagggct ttccgcaccc gaaggcctcg aacataaccg
     5821 tcgggttgcc ctcgctaagg cttggcagga caaagaggtc acaggcgttc aaccagagcg
     5881 ggatctcatc gtgcggcttt cccccggcaa gcatgacgtg atcttcaaga ccgagcgacc
     5941 cgatctggct ttttaaggcg tcgtagagtc tgccgggacc gacaaccacg caaaggatat
     6001 ccgttcgctc cctcacgatc cgcgacattg cattgatgag gtgctcatgt cccttgacca
     6061 caccatacaa cctcccgacg ttcaggatca cctttttgtc tgtgggtaat cccaacgcct
     6121 ctctgcattc ccctgtattc atggggtaga actttgtgtt atcgtagccg tttggaatgg
     6181 taaccacgcg ccggagcggg agggatatcc catgatcttc cgatgcgccg ttgtattgtg
     6241 caatgtgtcg ggcatctgac ttcgagagtg tcacagtcct gtcaacggaa tttaagataa
     6301 ccctccttgc actcttctcg taaatccgca cgcatgtgtc tcctatatgt tcgccaaaca
     6361 tcaactggcc atgatgcgtg aggacgagcg gcaccttgca gagtctcttc aataagggaa
     6421 aggagagagc aacaaaactg tagacgttat gaatatggat gatgtcgaag ttcttcagtt
     6481 cgtttatgag gagaaagatg gatggtacaa ggggatttct caacggttcc ccgatgcatc
     6541 tcagccttct gacgctgata ccatccataa cctcgaaggt tctgccttcc ggaatgtttg
     6601 aggttatcac ctcaaccgta tgcccctcgt tgatcaggcc tttgcttaag ttatagacgt
     6661 acatctccat cccgccatta tgcggtaaaa aatacggagt tatctgagcg atcctcatgt
     6721 ttcacctcgg ccgatgccga tcccacccgt ctcacagcag agcgctcgcc ccggttacgg
     6781 tttcaaagtt ccaggtgaag cgtgccgcga aagtcttctc ggggaccaac gctgccggta
     6841 taaaccggtg gaggacccca tcccccggag cccctgacct caggggcgac gccgcgcttc
     6901 cgcccggacg atgtaagggc gtgatccggc tttccgtgag aagtcgatac gacggggttc
     6961 ttcatcgcac ctgcaggcgt gcctctggga catgaggcga atgagtatct cctgtatccc
     7021 cgagattctg tgagtgttat gggcgctggg tccgacatgg agggggatgc ccttccggga
     7081 cacctgatat aactttcgga ttaccgcccc gtaatcgtaa ggtcattcaa ctaccccttc
     7141 aacggggcct ttccctgatg aacggagaaa aatagctctt aatcggtttt gcggccgata
     7201 taagaaccgg cgtatatggc aacctttccg atgagatcat ttctcacatg ccgtgatagg
     7261 atcggagggc gccgaatgga tccatacccc atcatgcagg tgggtaaaag aaccccggca
     7321 atcatgaatg ggggcggcaa agacctctct gttcttttaa gataggatcc cggaatgcag
     7381 gggccgggca agctcgcccg gatcgggccc ggtgtaagga ttgcgctggc gattgccgga
     7441 tgtgcatacc agttcttccg gtgccggatc ttacgcagat cctgtcggat atgcctgttg
     7501 tatcttcttg ctccccgtca ttgctggagg tggtagatgc tgatgccgct gttggagtag
     7561 attctatcct ctgtggataa tctgccagta aggttcagga cattcccctc tgtaggcagg
     7621 tagggttgaa gaccctttcc cggatcgaat tttgtctcat ccatcgcttc ctcatctcca
     7681 aagagcagtc cgccaccccg gagttgtgcc tccaggagaa taacgtatcc actaccccag
     7741 gagcaatttg ggtcatcggg gatgaggcga ccggtatagt aaggcaacct cagatgctcg
     7801 gattcgctta tcttctttct cccgaagaat cgcagcgtat agtaatccgt gtgaagcagg
     7861 gacccgctcg gcaccttctc caggatatga tgaaacccga tcacctcagc ctgattgaaa
     7921 gaataacgga tgtaacccgg gtcttccctg ataaacccaa gggcgctgat gccatagaga
     7981 gcaatcaaaa cgatcatcac ggccccaacc cgtcggggcg atagacccag ggcgaccagg
     8041 taacgcatga ggatatagat gcccacaccc atggcaacgg taagaatcgg cagaaatagg
     8101 atagcgaatc gctcgatgcg gagaaccgat accatctgaa acatcacggt aaagataccg
     8161 ggaacattca ggatgatcgc taccatcccc aatatgccga acacgaccga gtattccggc
     8221 cgctgcttcc ataagagata aatcgctccc agcagtgcaa agaacacgat aaaaagggta
     8281 tctacctgat tcaggagaaa accccagccc tggtgtaccg cttcggtgag agcgaggttc
     8341 tgataaatgg atgagtcgat gcgtgggagc aggtcctcta tgaaggggta cgctataaag
     8401 agccagtaac tgagaaacag agagacgggg accatcagga aggttatact cacacgtctt
     8461 ctctcattgg tgagtcgctc gaagaggtag aggagtccga tcagggcgat gatcatgggc
     8521 attgagatct gatggatcaa caggatgaac agggccacga tcacggcaaa ggccctccgg
     8581 gtggtggatc cggtaacggc gtactccgct tcgggtctcg tctccacgat agagtagacc
     8641 agatacagga gaatcagaaa cccgacgtag gccatggtcc tcgtcaccat atatgtgccg
     8701 tagtatatga catcggcatt catcgcatac acaagaacga tgagaagggc gatctgatcg
     8761 ctctgaaata tccttttgat gaggtaatac agaaacagca cggttgtggt gaatatcagg
     8821 cccgtggtga tgaagagaga agtttcgata ttcaggccga tcacgtgtga agataacgcc
     8881 acgaagacgt gatagagagg aaagtacgtg tacgttccga gctcgccggg tatgatatgc
     8941 cccgatacat atgtgatggt agccatgtaa ctgtgcggca taagatccgt tgtcccgaaa
     9001 tacagggccg gcctcagggt ataactgtag atcgtgactg cgagggtgag catgatctct
     9061 atgagcacca ttaccggccg taaccgcgca gataggatct gggtaaatat taccgcatac
     9121 aggatgagca ccccaacgag gcccaatccg gattcgggcg gggtaatgag aaaaccaaga
     9181 agagtgagag taaagatggt cagaaacagt cgtaaagaga tcaggcggtt gtaagaatat
     9241 acctcaagca cccctgaaag ttcgatatcc ttctttttga cgagaaggaa tgcgaaagat
     9301 ccgatgattg cgggaactgc aaggatcgag ccgttgacca ggtaactcat ctttccgata
     9361 aggaggcaga cagcacaacc tgcaacgtag gtgaagagta ccagataagg aaagatcccg
     9421 gtcagccttc tgaatagctg aactctcaat ccgaactctg cctccatacc tgcacacccc
     9481 ctcctccacg ctcaatgctg ataaaggtga gtccggatca cctccggcac ttgtggagcc
     9541 ggggcaactc tgatgggttc gtggaggtaa atccggtgtc gtatgatggg tctgaggaca
     9601 atgcccggaa ggccggatgg tgttacgccg tgggcaaccc tacctcgatc gtcggaaagg
     9661 ccgtttacag atccggggtt caaggggtgc aagaaagccc ccgggcttta gcccggggag
     9721 agtgtcaaag ggtccggtca gtaatacgaa atcggccctg attcggagaa gatcccattc
     9781 tgcccgatca ggttctcagt cacaaagact ctttcctgcc cgccggtctg ccagaccggt
     9841 ggtgcctggg tatagtagaa ccagttgtgg tcgatgtacg ctccgtcgcg aggaacgccc
     9901 cgaatcgcta ccgggaacga gctcaggggc cccgtggcca tgaatgtatt gtgatgaatc
     9961 ttgatcgtgt ctccggcgat cgtggtggac ccggtgacta caccgtgcat gtcgaagttg
    10021 tgagaagaac ttacgatgaa gttcggcccg cagatgttgt atctcgcctc gtacccatct
    10081 ccggcgaccc cgctcccggc gatcgcgtgc cgacagtaat cgaagtaatt cgcctcgatc
    10141 agactcgttg ctcctcccga caccacgacg ccgtatccaa ggccctccat ctggcagtgg
    10201 tggatatagt tgtgatggat atacccgccc gccttcatat cggaaccccc agtaccggca
    10261 atgccgatcg cagcaccgtt ccaaccccag atctcacaat tgtccacctc aaggttgcga
    10321 taggctgagt ggattccaag ggccgacgga acatccgttt cgttcccggt atccggcccc
    10381 tcaaggcgga ttccggtaat cctcacattc tctccaccgg cgataaatcc gatcctcgga
    10441 tcggagtccg ccgccctgag gtagatcaac cctcccggcg agaccgggta ttcgccccgg
    10501 gccgtcgagt tgcgctgagc cgagatatat gccccgttcg atgctgccgg atctgcaatc
    10561 ttctccaggc cttgcgatag atccccggac tccgcttcta catggatatt atctctccca
    10621 ctctgcccgc ctgctgcagt atcgggaaga tagccaatat caccggttat gagaaccgcg
    10681 tcaaggctcg gatcgtcctc acgccagtag atgctcaggg tgtgcttgcc gccggagagg
    10741 tagcgaacct ctccggtgct ccagtgccag tcctgcccca cctcatccag atcccatcga
    10801 cggatctcct catcatccac cgagacccgg atggtgcctc cgcccccatt taatccggat
    10861 gcgcgaaccc gaaacgcata atctcccggt tctttgatct caaatgcata tgttgccgat
    10921 ccggtcacgc ttcgctctcc ccgattgccg gcaagcgtaa cgcccggggg tatgaccact
    10981 cctgccggag ctccggtgag gttgatccgg gcagtctcat cgatgtagat gatatcccca
    11041 ctctctgcat ctcgaagggc atcaaacagt tcgccggcgg ttgtgacgat gttgcccgcc
    11101 ccgggctgcg tcggtgtgag tatatccttg tagccgtctc cgcccccgat agggctgccc
    11161 gtggggttcg ccccggcgcc gaaagcctgc ttatactcag tggataccat tgcaggatcc
    11221 ggatcatctg caggatacgg ggtggaggtg gagtgcatat ccccgcgacc ccggggtatg
    11281 ggatcgagcg ttgccgggat agcctgggct atacagagaa agaagagtat aatgatgagt
    11341 atcgcctctc gggagcgcac ggtatttgca tctctgatcc ccacgatccg ttatatgttt
    11401 tgtcgggcag cgggttttca ggctatggcc cgatctggtg cacccggaat atccgtcggg
    11461 atacgtgtgc tgcttttgag ccatgcattc aggttccttg cctgatagca aaatagggtc
    11521 gcaccggata ggtgcccggg ggtggagagg aaaaagccct atttctgaag aaggagaggg
    11581 tgttggaggg gcacctttga cctctcctgc ccgctcttcc cgctcagtag tacttgattg
    11641 gcccggatgc cgagagagtc ccgctcggtc caatcaggtt gttcgtcatg ctgatcccgt
    11701 ttttgccacc tgtctgccag accggtgcat cccgggtaaa gtagaaccag ttgtggtcga
    11761 tgtacgcacc gacacggggg acaccccgga tggcgatgca cgtcggcatc tgagaggttg
    11821 tacccaggaa cgtgttgtgg tggatcttga tcgtgtctcc ggcgatcgtg gttgacccgg
    11881 tgactacacc atgcatgtca aagttatggg gcgacgtcga gatccagttc ggaccacaga
    11941 tgttgtaccg cgcctcatac ccatctccag cgttcccggc tccggtgata ccgtgccggc
    12001 agtaatcgaa gtagttcgcc tcgatgagag cgacaccgcc actggataga cagacaccgt
    12061 acccaagacc ggccacctgg ttgtggtgga tatagttgtg atggatgtag gcgccggtct
    12121 tcatgtcgga accgccggtt ccggcgatac cgattgcggc attgccccag ccgaagatct
    12181 cacagttctc cacctcgagg ttccgatagg aggtgtagat accgacattc gtcgaaccgg
    12241 atgcgctggt gctcttctgt ggcccttcaa ggcggagacc ggtaatcttc acattctggc
    12301 cgccgattgt aaacaatcgg gtcccgctcg atgtctggta gatcctgccg ccatccgatc
    12361 cgccctggcc acggtcgctg gcaagggtga ccccgctgcg tatcgtggtg gttcgtccgc
    12421 tcatgtcgat gttggcattt ccttcgaccc agatgacatc tccggactgc gccgagctga
    12481 gtgcgctcac gagtttggct gtcgtgtcga cgatgaagtc tgcgtcactc cggctgacgg
    12541 tgctcttata cccgttacct cccccgatcg ggtttccagt agggttggca tctgcaccgt
    12601 agacattctg acccggcgtc ggcgtaggag tgacggtcgg cgtcggtgtc ggtgtctttg
    12661 taggcgtcgg cgtaggggtg acggtcggcg tcggtgtcgg cgtcgacgtt ggggccttca
    12721 ccgtcatcgt cttcttgagg gcgttgttgt tctcgttact ctccgcgatt cggttcacat
    12781 catcgaccgt cgcggtgatg gtgtacgtgc ctgcaaccgc cttccaggtc gagccggaga
    12841 aacctccgtt cgcggtcacg aggacccatg cgccgggggc gatcgatgca atgtaagtgt
    12901 cagaccagac cgcggaacca agattcccat cggccgtgaa ggcaacgcca tgaatcgtac
    12961 ccgcaggcgt cgcaccagtg ccctggttct tgatcacggc cttcatcgtg atcgtatcgc
    13021 cggtcatcgg gtttgccggc tcccaggaga tgtcggtcac cacaagatcc ggttttccgg
    13081 agggaactgg tgtaggggtt ggggttagtg taggagtcgg ggttggtgta ggattggata
    13141 ccctgatccc agcgggctcg gtcagaacat aagttcctga ctgaaggagc acaatgccgt
    13201 tactgatggc cttcgccgca gactggatga ccgtgctgtc gtctatccct gcagttccgc
    13261 tactgatcac ccgaccattg ctgtcctcag caacgatctt tgcaccgttg gtgtacacaa
    13321 caatatcgta ggtaccctgt gatacctcct gactttgcac ggccagtgcc ggtgtcacca
    13381 tagcgatcgc catgcacagc gccagtgcaa gaaatagggg tttcttcatg ttcattctcc
    13441 taaatcccgc atgagaggag cccatcaggc atcctggcgg gcaggtgagg gtagacccct
    13501 cagaattatt tcactctact aacatatata tatgtatgat tttgaattct gtttttaaac
    13561 gtctgatatc gctattattg cgttatatat ctccgattct ttccagattc atttataata
    13621 gagcaatttt tagaataaat atttatatta cctcgaatgt atgactctga aaccagcatt
    13681 tcccgtgaac gattttctcg atcttcttgc caggcgctgt gtacctctgg accgatcaat
    13741 gtggcaattg gaatcccggt aggcatcagg caggtgtaat ctgccggtac agggtcccct
    13801 caggataatc cggggataga tccgcagccc aagggttact gtccagcaaa acggagacgc
    13861 aaaagattgc tcggatggaa ttccggcctt gacgacgtca agatcatatg gtgtccgtgg
    13921 aaccgcaggc acaccgaatg agaacggagg ggcatttcca cgctaagggg cgtatcccgg
    13981 caagaataac gcttcaggga tggggggccc ccttcactcc gcccgcacgg ccgccccgtc
    14041 gttcctcgct cttgtcggga tcaccgtccc cccgcggtcg ccgagcacct cctttgccgc
    14101 ccgggccaca tccgccatcc cggaatcccc gacggcatag accgtcggcc cgaacgagct
    14161 caggcccgcc ccggctgcgc cggcctcgac catcgcctcg atgagcgagg agacgaccgg
    14221 gtgctgcatc gcgacctcga ccttcttgaa cccgaggacc tgcgtcctgt tgatcgccga
    14281 cccaaagagg tcgatatcct gctcgaccag gcccgggatc atccgcatga gcacctcgtg
    14341 gcagagttcc cgcacctcgt gaagcggcac cgggcagtgc gtgcggaaga tatcgatctc
    14401 ccgcctcccg tgcgccccgg catcgatatc gggggtgacg agcaggatgc gccagtcttc
    14461 cgggaaccgg tgccgggcga ggaccggcgg cggtgcgacg ccccgggatg ccgccgaagg
    14521 acggaagtcc tgtttgcccc cgggagagcc gaaccggtgc ccgccgtcca gtatgaaccc
    14581 gccctgcgcg aacgccgccg tgccgatacc ggatgttccg ccgcggccga cgatccgcgc
    14641 gagatccggc accggaacat tccggtcgta gagcctgcag agggcggcgg ccgtcgcgag
    14701 agcaacctgc gtcccgctcc cgagccccgc gtgcgggcgg gccacggcat gcaccgtgat
    14761 gtccgcgccg ccggggagac cgagcccctc cttcaccgcc cgggcagcct caagaacccg
    14821 gttgcggaga agttcatcgc cgccgtggac atcgagcccc ggccctttgc gcgcatcgag
    14881 gagacacccg ggctcgtcga gagcaacccc gacaccgccg tccaccctcc cggaggtgcc
    14941 gttcatgtcg agcagcgtga tgtggagccg ggacggagcg tcgaccagaa ccctgacatc
    15001 gtcggcaaac gccgtgcagg ggaagatctc ctcgatcgcg atcagcggtt ctccgcggtg
    15061 gatgatccgg tacttccgcg agagcatcga ttcgcaccgg tggatgttga acgtccgggc
    15121 gaggtccgtc ccggcctgga cgacgcgcgc atcggtaatc tcccggcggg actctatccg
    15181 gtggcgagaa aggatcctcc ctatggggat atccgcccgc atgagatcct gcctgaactc
    15241 cggggcaagc cgccgcaacg gtgtgcgcga gaccgcgtag atgaggacgt ttcccgtaac
    15301 gctgtctttg agctccacga tgcggtggtt gacctcatcg ccgggttcga tcgcaagcgc
    15361 ggccgccgct tcagtatctg cggcgacgat atcctggacg cgggtggtga tgctgacgga
    15421 gtgaccggtc gccatctcaa ggaggtttgt caccgaaccg tcggtcccga tgaggacctt
    15481 ctgcatcggg gagagctttc ctacgcgctg ctcgatctca tggatctttg cgctgatcct
    15541 gtctgcagtc aggggttcca tgccagaata cgggtactgt acgcgcagac tacaaaaagg
    15601 tggtgccgga gtccggcctg ccggatcaac acgagaagaa gagggagagg aagtccggag
    15661 cctgcacgag tccaaacgac ccctttgcag catcgcgttc ggagaacgcc cggacaccgt
    15721 ctctcccctc cccgagcatc gcaaacggca ccgggtcggc agtatgggtc ttcagccgta
    15781 tcggcgtcgg gtggtcgggg aggaccgcaa cggccgtctc cggccgatcc aggatgatcc
    15841 cgatcgcctc gtcgagccgt tcgattgccc gtaccttctc ctccacgctc cccatgtgtc
    15901 cggcctcatc gggagcctcg acgtgcatgt agacaaagtc caggcgatcg agggcatcga
    15961 ccgcgtaccg ggctttcgcc tcgtagtcgg tatcgaggaa ccccgttgcg cccgggacat
    16021 ggattacctc catcccggca aggcgggcga taccgttgag gaggtcgacg gcggagatca
    16081 tcccgccgga aagaccgtat ttctcacgga aaggcgccag cgacggtttt tccccgccgc
    16141 tccacggcca gatcccggtc gccggggcct ttccttcatg caggcggcgc cggttgaccg
    16201 ggtggccggc aaagacctcc cgggaccgct ccatgcagtc gaggaggatg gcggcatcgc
    16261 ctccgcgcgg gaggtagtct ctgacggcct ggccgacgat gtcgtggggc ggggtggtga
    16321 cggcgccgcg cgccccggga accaccataa ggttccggta accgaccccg gggtagaccc
    16381 tgacgtcttc gaatgcggcg tcgaggtcgc ggaggaggcc ggcgccttcg gtgctggata
    16441 tgtgcccggc gttgaagtcc tccatcaccc catcgcggat ggtgaccagg ttgcaccggt
    16501 aagccatctc ccctttccgg aggtcgaccc ccatactggc cgcctcgagc ggccctctgc
    16561 cggtatacga ggtacggggg tcgtagccca gtacggagag gtttgcggta tcgctcccgg
    16621 gctcaaaccc atccgggaca gtccggagca tcccgcaccg tccctcacgg gcgatgcgat
    16681 ccatattcgg tatgtctgcg tactcgaggg gtgtcctgcc ccccagttcg gcaagcggtt
    16741 catccgccat gccgtccccg agaatgagga tgtacttcat gtgaatcatg cgaatctgct
    16801 gtgtaatctc gatagtgctg gttactccaa tccggtcgcc ggggccggcg acgaacgccc
    16861 tgccgccggt cttgctacag gaagcgcccg gctagggccg cgcaccttcc ttcccgagca
    16921 tggcgcggac cgccgtacgc acgctctctt ctgacgtggt cccgcaccgc cagccgaggg
    16981 ctttgagttt ctcgaccgag agctgcatct tcggcacatc gccgacccag ccgcgtgcgc
    17041 cgccggtgaa ccggtaccgg acgccggaga gccccatctc ttccgcgacg atatcggcga
    17101 tcgtgacgac gtcgatccag tcctcagagc cgatgttgaa ggtattcgcc ggatcgtgcg
    17161 aatgatcgat cccgaactgc acggcgcgga cgcattcgcc gacttcaagg tacgacttcg
    17221 tctgccttcc gtcgccgagg atctcgagtt ctcccgggtt ctcgcggagc ttccggatga
    17281 agtcggatat gacgccgtga ccgctccgct cgccgatgat gtttgcgaac cggtagaccc
    17341 acgcccgcat gccaaacgag tggcagtacg acgatatgag cgcctcgcag gcaagtttcg
    17401 tcgcgccgta gacagatatg ggttcgagcg gcgtataggt ctccggcgtc gggatgaccg
    17461 tggcctcgcc gtagacggtc gacgtcgagg tgaagaccag ttccggcacg tcgtgcgccc
    17521 gcatcgcctc gagcacccgg tgcgtggcaa tgacgttgtt cctcacctgt gagtccgggg
    17581 ttacggcgct ctgccggaca tcagggtctg cggcgatgtg gtagacccgg tcggcgcccg
    17641 caaagtgctc ctgccagccg tcgtcgagca ggttctgcct gatgaaggcg acccggccgc
    17701 ccgccgcatg ccgcgcgagg ttctcttccg tgcccgcgct gcagtcatcg atgaccagga
    17761 cgtcgttccc ctccgcgacc agagcgtcaa cgatgtgcga accgataaaa ccggcgcctc
    17821 ctgttacgat acagaacatt gtagagtaat agccttccca cagtatagga tttacttatg
    17881 gggagtgccc cggggcagcc tcgttcccgg gtaaccggag gtctcgcccg ccgggcaccc
    17941 cgaatacgac gcgacgatag tggaaaaatt tatatctgcg ggggatatca gggaatagag
    18001 cggcactcct ccgccgggag ggggatggtg gccgcagagc ctcgcctgta agggcacggc
    18061 aggttgcacc aggacatatt ttttcagggg tctcgccaat atatggagaa ccataaggag
    18121 ttgtccaatt gagagaggta tgcatccgta gcagtaccgg tccgttcccg gccgccgggt
    18181 ccggtggcat cccgtcgccg ggatctgtag acaccgatca cggagccagg ttatgagcaa
    18241 cccgcgcccg gatatccggg gacacgccgg gatttacctg cgcggcctga tgatgggggc
    18301 ctgcgacatc atccccggag tctcgggggg gacgattgcc ctcatcaccg gcatctacga
    18361 gcggctggtc ggggctatcg ggagtatcga cccggcctcg ctaaagcacc tcgcgagagg
    18421 cgacttcgca tccttccgaa gggatcttga gagcctcgat ataccgttcc tcgtcgtcct
    18481 ccttgccggg atcggcactt ctttcctcct gatgtcgggg atcatcctct ccctcctcac
    18541 cgatcatgcg gtggcgacgt actccttctt ccttggcctc atcctcgcct cggcagtcct
    18601 catattcctt gagatccgct cccccggtgt ggcgaccctc gcttatctcg ccatcgggac
    18661 cggtgccggc tacctccttg ggggcctcgg caacttcgac gtcggccact ccctgccggt
    18721 cctcttcctc accgggatgg tggcgctctg tgccatgatc ctccccggca tatcgggagc
    18781 ctacatgacc ctcgtcctca accagtacga gttcatgctt gcggcgctcc gggccctctc
    18841 tctccccgag atagtcgcct acatcgccgg aggagtcacc gggctccttc tcttcaccag
    18901 gggcctcaaa tacctcctta agacccatcc agaggcgatg ctcgccctgc tcaccgggct
    18961 gatgctcggt tcggcgcgga tgctctggga gaagggatct cttgccggga atatggtggc
    19021 cgggggctcg gtcttcttca ttgccggcct cgtcgtcgtc ggggcagtcg agtacctgaa
    19081 gaggcgctac gcgggcagcg gggcctgatc cgcgccccat ctcccgccgc gatcggaaag
    19141 gatactacct ccggaaggaa gaagtacagt atcatgttca tcggcatcga tcacggcact
    19201 accgcgatgc gcttctccac cggaatgcag gagttcaaga tctcccgcga agaagccaga
    19261 gacttttcgg cctccgacct ctcccgtctc tctcccctcg aggagatcga gggcatcgcc
    19321 gtctgctact ccatgggcga cggcatatcc gccatcaccg atatcaggaa ggtaccggat
    19381 cggggcatcg tctcccgcga aggcgccggc aagcacatcg gcggcggcac ccgggtttac
    19441 gatgcgatca aagagagcgg gcttcccgcc gtcgtgatcc ccgggctgca ccgcggctcc
    19501 ccgaccgacc cccggttcaa ggcctactcc caccaggcaa gcccggagaa gatcgggatc
    19561 ctgtacgagg tcgtgcatga ccttgggggc gacgtcgtcg tctgtgacgc gagctcaaac
    19621 accgtcacgc tcctcgtcac cggcggccgg atcaccggcg cgttcgatgc ctgcatcttt
    19681 gcgcccggca cccggcacgg cgccctcgac gtggatgcca tccgccggat cgaccggggc
    19741 gagtccacgg caaacgatgc cttcctccac gcgggcgtgg ataacacgat gccccccgat
    19801 ctgcgggtgg aaacgatggc gatgtttgcc gcgatggagt gcgccgcaat gctcctcatc
    19861 aaccccggtg ccagggtcgc ccttgccggc tccatggcgc ccgccatcgc cccgggcgtg
    19921 aagacactcc ttgcccggga tgtcgcggtc tacgacgagt ggtgtgcggc ccggggcctt
    19981 gcgcggatcg cccgcgacgt cttctcgggg gcgaccgggg tcctcgggct tatggtggat
    20041 ctctaacctc ctctggctct tgtaccccat accatttagc ctgcgggggt gaaggggtgg
    20101 agcgaagagt cctcacgcac ccggagaaca cgtatcagaa aatatatatg ggacactgac
    20161 tgaaagtgca attgctattc acggcacagt cgttgcttat cggcaacggc tgtttgtgta
    20221 atcgcagagg atcttgttct ggttatgatc gggaggataa acaatatgga acagcagttt
    20281 gcggggttta aggtcggaga aagaaggaag atgatcgaca ggagggagtt taccaatctc
    20341 gatggagagg taaattatgt tgccctgatg aataaatttg acgaagtcgg tgagagacta
    20401 cttgaggaat caagactctt gaggcgtcac tgagggactg cttttgcgta aacaccatac
    20461 ttcgctttcc gaatgtgact accccggcat atgcactctg ctctccggtg caggctattt
    20521 ccgtggcttt tctgaatatt cgtgccattg cgtgaggaaa aattcggttt acagccggga
    20581 gtgggaccgt gaatatcgcg gttacttcag ccatgagtgt gaagggatcc atatcggtgg
    20641 atcgatgctt aaggtggctc tagaccgggc aagcatccca caggacgaaa aagaaaaggt
    20701 tgtccggata atcgagcaat tagaggatat ttctatagat aattcgataa gccggctttc
    20761 catctcaatc aacaatgata gactggggta tgtcgacctc accccattca tgcaggtgtg
    20821 cgatgcattc ttaaaaccgc cgcattggat gcgtccccca acttattacg tcacctgtgc
    20881 aaagccgtat atcccggaga ccaccctgcg atacaaaagg ggtgaaatag acgagatcaa
    20941 ttgcgcccct tatatcaaag tgaattcagt tataggcgtc tgcgcacagt atgctatgag
    21001 aatggctctg atgattctgt cacccaaagc cccgacggtc ccagaactca tatccgaggc
    21061 cataaacacg gcgttatccg ggggcgttga gagagaacca gatatagggt ggcatccgga
    21121 tgagatccac cattttttcg agataagtgg ttatggcgtg tttaggtaca gtcgctctca
    21181 gttaagatgc gataactgtg gccaccttgt ttctgatatg cgcatggttc ccggcattga
    21241 gaatatatac gcctatgtag aatccggcct tcctgtaatc atcggcgtca gaaatacaaa
    21301 gtatctcccg tggtggggag atggggatga ggcccatgct ctggttgcaa taggacatac
    21361 gcttgatgga aacgggaggg ttgatgggat tatcgtccac gatgagagca cgtacccata
    21421 tcagatcctg aaagaaccgt tggcgggtgg aaaaacactg gaagatgtga tctttgaggt
    21481 aattgcgccg gtaccaagag aagtcatggt tgaataccag gtcgcgagga gtttagcgac
    21541 gcaattcttt gatctggatg aagaggatat catcaggccg gttttaatcg actccaacca
    21601 gattaagaag tggttggggg aaggtaccag gcggcggcac cttgaagcgt atgatcttcc
    21661 agccgtcgtg aaagaggact tcctgagcgc gtatatggat agatacgtgt ggctttttga
    21721 aatgaggaag gagaggggtg acaaacgaga atatgtcggc gacgtcattg tcggggcgat
    21781 aaacccttcg attttaggct ttgtcatgcc ggagcagagg atttacggtt tcagagatgg
    21841 ctcaaaggga cctcatgcaa aacatttcga cgatgcagtt tgatgtgcgc tccatgaccg
    21901 aaaccaggtg atttttatcg ccccgggcgt gaagacactc ctcgcccgcg acgttgaggt
    21961 ctacgacgag tggtgtgcgg cccgcggcct tgcgcggatc gcccgcgatg tcttctcggg
    22021 ggcgaccggg atcctcgggc tgacggtgga ccggtagggg ccggcccggt gatgctctcc
    22081 ccggagaggc gtctctctgg tttcacaccc tttatatgcg gccgggtcgt acactggact
    22141 gaaagaggag gagccaacag tatgcagctg gaatgcccca gtatcgccga cctcatgctc
    22201 cagatggcat cgagcctcga ggatacggca cgcaccatgg accagggaaa tgcgaaccga
    22261 tttctcgacg agatcctcaa cgcaaagagg atctacctgg ccggtgccgg gcgatcgggt
    22321 ctcgtcgcgc gagcgttcgc ccagcgcctg atgcacctcg ggtttgagag ttacgtcatc
    22381 ggcgagacga tcacgccggc gttcgggcgc gaggatgtcc tcattgcgtt ctcgggttcg
    22441 ggagagaccc gatccgtcgt cgacgcatgc gaaaccgccc gggagatcgg ggggaagatc
    22501 tgcctcatca cgtcgactcc cgactctcgc atcgggcaca tggccgactg catcgtcgag
    22561 atcgggaaca atcggttcaa agatgcgaat attccaagcg attttgagat ccgtcagctc
    22621 accggacagt atcggtcggt ctcttcatcc ttcgcccccc tcgggacact ctttgagacc
    22681 gcggcgctgg tcttttctga tgcgatcata tccgccctga tggaagtgaa gcattgcacc
    22741 gtcgaagaac tcagggaccg cctcgcaaac gtccagtaga ccggggtcgg tgttcccggc
    22801 atcatggtac cctgcaccgg cggggccacc tcttttataa ggatccgctc tttctgcctc
    22861 cgatcttcac gagatttatg ggatttccgg gcgggcatga ccacaccggc gtttctgcgc
    22921 gcatgggggc cggccgcatc cgggtgcaga ggtgtacgtg cccacagcgc cgcacggacc
    22981 tatcctccaa tggtattttt cggcaggcac gatccccgcc cgccgcagaa ccggcattct
    23041 atctttcgga gatcggttcc gtcgacggtt gatctgctcc ggaagtaaat ggaaacatat
    23101 ttcttttgat aaaaagtaaa ctaaaatcgt ttacgggccg gcgagcgccc tccaggcgcc
    23161 cgaagggagc ctgggatgat ttcatgacac gaacggcgaa tctgttcctg gtaatcctga
    23221 tactgacggt cgcgctgctg ccggtatcgg cgatggcgga ggcgggaact accgagctgc
    23281 gtatcgtgag gtacgcgagc gacaaccgaa ccgtcctcga tgagacgacc gtcgactatc
    23341 tctggatgaa ggcgaacctt cccgtctacg gggacgggag gacgcattac ttccacgagg
    23401 ggccggtgct cgaggagtac tgggagaacg cccatcccgg cgaggagtac gatccctggg
    23461 atccggccga ggacgtgcag gggagcgtgc tgcagaaagg cgacctcggg gccgtgatgg
    23521 ggaccggtgt tacggacctc tgcgctctta tcggcggggc ggaggagggg gaccggatcg
    23581 ccgtcacctg ccgcgacggg tggcggaaga ccttccccta cgagtatatt taccgccccg
    23641 acccgcggca gggtccgctg gtgatctgct ggttcaacgg ggacgaggct cccggcgaga
    23701 agcagggggt cgggtacccg gacaccacct acttgtcggg gatgcgcatg gccttcttcg
    23761 cggacacctc ctcgaacccc tggggatggc atatcttcgg gaacaacgat atgcgggagt
    23821 gctgggacga ggagtactgg aactacgggg cgcaataccc ctcggctgtc ggagtctcgg
    23881 ggaaatgggt cagcgagatc gggatcttca ccgaccggct gcccccggtg cccgtcgcgg
    23941 agtttgagac gaacgtgacg tccgggaggc tgccgctggc cgtcgcgttc aacgatacgt
    24001 ccaccgccaa cccgaggact tggacctgga acttcggcga cgggtcttcg tccgcggaga
    24061 agaacccggt ccacgtctac accacggccg gggactacac cgtcaccctg acggtttcaa
    24121 acgtcgccgg gaccgcgagc gtgatccggg ccggcgccgt caccgtcgag aacgcagtgg
    24181 ttccgaccgc ggacttcgct gcgaacgcga catcggggga ggctccgctc gcggtcgcgt
    24241 tcaccgatgc ctccgacgga aaaccggcgg agtgggagtg ggacttcggc gatggggaag
    24301 gtgcaaccgg gccggccccg gttcacgtct attcgtcgcc agggacctac acggtcgcgc
    24361 tcacggcgac gaaccacaag gggtccgata cgatcacgaa gaccggtgcg atcaccgtcg
    24421 acgtgccggt gcccgcaccg ccgatagcgt cgttttccac cgatgtgagg gccggggatg
    24481 ctccgttcga ggccgcgttc accgacacct cgcagaagtt cccgacgacg tgggcctggg
    24541 atttcgggga cggtgcctct tcagaggagc caaacccggt ccatatctat tcgtcaccgg
    24601 gggtctacac ggtcgcgctc acggtgacaa acgacctcgg cagcgatatg gtaacgagac
    24661 cacagttcat cgcggcgacg gagaacggga aggcgatcct cttccgcggg acggtgccgc
    24721 tcggcgaggg gacgttttcg gtcaaagcgc cgtcaggaca ggcctacacc ccgaacaagc
    24781 gcaccccgat gggcgcgctg gatgccgtgg cagagatgcg ggggttcacc tacgtgatcg
    24841 gggacaagaa gtactcggag agcaggctcc tcttcctcga cggggtgggc gagtacctgc
    24901 tcgaaaagtc gaccgggaag acctgggtct gtttccataa cggcgagctg ctcgacgact
    24961 acggcaggcc ctcgaccgat gccttcaacc ggcggccggt cgcggatgga gatacggtgc
    25021 tcttctggta cggtgactac ttcatctata gcacggggaa gatggaaccc aggggagggg
    25081 gagtgtgtct tgacaatacg ctggcgatcg tcgagatgac gatcgcgtcg gagcccgcgg
    25141 caggtggggc gaccgcggcg caggccgggg ccgcgagcac gagcgcgccg gcgccggagt
    25201 cgcccccggg cgtatggctc cccatcctcg caatcgggct cttcctggtg ttgcgaaggg
    25261 tctgatatcc cttttttact ggagagaggg ggactttgcg gggtgagccg gggtccggat
    25321 ctacaccacg agcacccgtg agatcccgct cgccttcgca cccggtgtgg tcaccacgct
    25381 gcctgtaaag cacttcaccg tagttgggat tgactatcgg tttcgcagtt ctcgcgacac
    25441 atcaatattt ccgatagacg ttactccggt ttccaatatc gatcacggta ataatcatca
    25501 cattgccctc gaagacaaga atgatccgat actgtcccac acgaaaagag tacaacggac
    25561 tgttcgggtg tcctttcagt tttttggcgt acaggtgagg gtacggctcg tttgccaggg
    25621 cgcggagttc gtccttgatt cgtgtggcat cgtggtccgg gattttattg agtttctgct
    25681 cggcccttgg catgaggagg agccgccagg tcatcagatg cctcgttccc gcacataatc
    25741 ttcaaagggg cgacccctga taccggcccg catgtctgcg atgctttctt cgatctcctg
    25801 cagtatttct tcgctcaaag gctccggatc gaagaacgcg tccatgatgc ggttgagcgt
    25861 ctcgtcgtaa ctctcccgag gatgtgtctt cagggtatcc aggcgggact tggtctcggg
    25921 ttggagctgg atcgtcgttg ccataactac ccataggtta ccataaagta taatgatttt
    25981 gtcagggttc cgttcagatc atcgaaccag tccgaggatc cacggcccat tccgccagca
    26041 tcgcagaagg gttcacagat ccggttattt tcgcggaccc ctccatcggt gccctgcgtg
    26101 ggcgggttcg gcggcctttt cctgaagacg ggggcgaaac gtcggcccgg aggatcagcg
    26161 cccattgatt ccgccggact ccgccgggtg cccttgcggg acgtccgcgc gcgccgggag
    26221 tcccggcacc cgcccggcga gtggggctgg ttccggtcct gagtggggcc ggggccgcga
    26281 gcacgagcac gccggcgccg cagtcgcccc cgggtgtgtg gctccccatc ctcgcaatcg
    26341 ggctcttcct ggtgttgcga aggatctgat ctccttttta ccgaaaaacg ggagacctta
    26401 agaggtgaac cgggcgccgc cccttttacg tggattacga aaattttgga ctcttcggcg
    26461 agcagtgatc gggagaaaca gtcttttgcc gactgtgccc atggtgcatc atttcatccg
    26521 ggcgttgatg caatcgaaac cggaaaaact atgtaaacaa tcatgatttt aattatatct
    26581 cattaactta aagagattgt ttatagaaag cattatacat tcccgcaccc catagcaaaa
    26641 aaccggttgc tatgaagctg attaccgttg caggccctcc gtcttcggga aagacctctg
    26701 taattctcaa aaccatcgca gccctcgggc tgcccgaagg gagcgtgggg gtcgtgaagt
    26761 tcgactcgct cacctctttc gatcaccagc gttacgacga acaacagata cccaatctga
    26821 ctgcgtttgc cggaaagatc tgcccggatc acttcttcgt cagcaatgtc gaaagggcgg
    26881 tcgaatgggg cacgaagaag ggattatcgg ttcttattac agaaagcgca ggcctctgca
    26941 accgatgctc gccgtacgtg aacgccatcc tggcggtatg cgtcatcgac aatctctccg
    27001 gcgtcaacac gccgagaaag atcgggccga tgctgaagta tgccgatatc gtagtcgtca
    27061 ccaagggaga tatcgtctcc caggcggaac gcgaggtctt tgccttcaac atccgtgaag
    27121 tcaatgcatc ggcaaccgtt ctcttcgtca acgggatcac cggccaggga gcgttcatgc
    27181 ttgcccgcta ctgtctggat gcccttgaca tccagacgct tcgcgaccgc aagctcagat
    27241 tcaccatgcc tgccgccatc tgttcgtact gtaccggcga gaccctcatc ggagagatgt
    27301 accagatggg gatggtcaaa agaatggagt ttgacgacgc atgaaggccc tcgattacgt
    27361 tgcggaagtc tctgccgtac cgatcggcga tattctccgc agatatcctc tcgctgtcga
    27421 ttaccttgcc aacgtgcggc tgagcgggat tgacgggacc aaaacactcc ccgagattct
    27481 ggaagaagtc gatgaggaga tgctcgagga gttcgggctt gaccgcgaag aggtcatatt
    27541 tcacttctgt gcgtttcttg agtcgttttc ccggacgaag ggaactgatg aggcaatctc
    27601 ctccatcacg atcctcggcg gacgggacaa ggcgggaaga cccgagatgg tcgagctgac
    27661 gataaccccc ggcgaggtcg taagcatcgt cggtcctacc ggctcgggga aaagcaggct
    27721 gcttgaggat atcgagtgtc tcgcccagcg cgatacgccg accgcacggc agattctcat
    27781 caacggttcg gttcccgatc tgaataagag gttctccacc gggggaaaac tggtagccca
    27841 gctttcgcag aacatgaact tcgtcatgga cttaacggtg caggaattcc tggagatgca
    27901 tgcaaagagc cggatgatcg ccgggtccgc cgcggtcgtg gagaagtgtt ttgcggttgc
    27961 caactccctc gccggtgaga agttcacgct cgatacgaag gtcacccagc tctccggcgg
    28021 gcagtcgcgg tcgctgatga tcgccgatac cgctcttttg agctcgtccc cgatcatcct
    28081 catcgacgag ctggagaacg caggaatcga ccggcgcgag gcgatcgaga tccttgtcag
    28141 caacgagaag atcgtcctca tgtcgacgca cgatccgctc cttgccctcc gtgccgataa
    28201 acgaatcgtc atcaaaaacg gcggcattac aaaggtgatc gagacgactg cggaagagga
    28261 gcagacgctt cagcagatcg aggcgatcga ttccatcctc cttacgatcc gcaatcagct
    28321 ccggggagga ggagtcatcg caaaagcgag tattccggca tttcaggagg gatgaacgat
    28381 gtgggaatta tacgatgcat tgattgaaga gatcccggac gacatcatcg tcgatgatgt
    28441 cgttgtcggg ggggagatga cgtacgtcgg ggcgaacggc aacgtcggtc ttgccgcata
    28501 ccgcgggtat gtccagcgtg ccccgatgat gaccggaaac aggattggaa agtcgttaaa
    28561 agaggtagcc gggtgcgtga agtcctggaa cttgctggaa gcatcgatcg gcaattcggc
    28621 gatcaacgcg tggtacaacc atccggatgt tgcgaagcgt gcagggatcg aggttccgga
    28681 aaaaaagcgg gttgaggagc ggttgaacga cccgttcatc aagtcccaga accttgtgaa
    28741 gggaaagaag gtctgtgtcg tcggccattt tccgttcctt gagaagctga ttgccccggt
    28801 ctgtgacttg agtgttgtcg aatgggatcc ggaagaggga gattacccat attccgcctg
    28861 cgaatatctg ctgccggaat gcgattatgc gttcctgacc tgtgccgccg tcggcgacaa
    28921 gagcatgccg cggcttctcg aactttcgga gaatgcagac gcggtgacga tcgtgggtcc
    28981 gggaacccct ctttcgtcgg tcttcttcga ttacggcgtc tccgatctct ccggcttcgt
    29041 cgtgaccgat gttatgctcg ccaaacggat cgtcgcaggg gcggagagcc agcggatctt
    29101 cggcgcggga atgaaggtgc agtaccttcg ccccggggtt tgagggagat tgcggcgcat
    29161 cgttgtcttt tagaacgtgc cgttctttca cgatcgccgt ccgaatggtt tttgtaacga
    29221 tctcgctcgc ctctttttgc tgaagatcgg agctcctctg agggtgtaat cccccgtgtc
    29281 cggccaccgg caaggttgcg gccccttgtt gggacggttt ccagaaggat ggcgtgctcc
    29341 tgctgtcgcg gcgctgaatc aatatcagac gattcccgtc caggagccgc tcgcccgggt
    29401 agaccttcag ggcgccggga agcacgatgc ccccggacca cgccgccggg gatgaccgtc
    29461 ccgatgccgc agacgttgac gcggcggtcg accggggtcg ttcccaccgt cccggcggcg
    29521 cggatgcgcc ttgcgaagcc ggaggtcgcg cggtgcgatc ggactcttcc cggtgctgcg
    29581 aatttacccg gaagcgccgc ggcacgtgac ccgggcggta cgtctcggcc gggtaccgtt
    29641 cccgtgccgc gctgcccgcc ggtcgatccc cgacccgcgg gcgaaacatc ggcccggaac
    29701 atcagcgctc gttgattcct ctggattt
//
LOCUS       DAOSEE010000007        29581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_748_length_29581_cov_212.717876, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000007 DAOSEE010000000
VERSION     DAOSEE010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 29581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29581
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_748_length_29581_cov_212.717876"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..461
                     /locus_tag="PLI76_01180"
     CDS             159..461
                     /locus_tag="PLI76_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60671.1"
                     /translation="MKTLEFHRDDAKGRVTVVCADREVSVYSYCGYCRHCAGVRVGKR
                     MIPTPQRQALSGLRQSANPDENLLNAAIMFNTLVRDGSAIECEDDKGEGFSSMYRR"
     gene            complement(640..>3008)
                     /locus_tag="PLI76_01185"
     CDS             complement(640..>3008)
                     /locus_tag="PLI76_01185"
                     /inference="COORDINATES: protein motif:HMM:NF023252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOI60672.1"
                     /translation="STTGSINGTLLAFTAPGPAEPLAAGATAELRFPVAIPENATVDR
                     GLLFVYVTDAKEAAAGADITPTFRFDGKPVMPAVTRDLQGTGSAPMAGTFSLAIPPEM
                     LAAGDHTLTVQMDAEPGATCRIPAALLVLVAADPGKPRIDYWIADGCDDVLADLWEGT
                     TADDATTEVKFDGAIDRARASAAHLYVVSTAGSGQGGTGNRVALNAWDWLNPLQEGPA
                     GVWTATLDALPFIQASDNTVEVQSYPIGAAGDHLQNRNAILVVRYEAESASPGIEVSS
                     GAGAAPEEEIGSFALAPEEEVRYTAEDGSLVLTFPAGCRATNSTGGAVAEVTVHAVRG
                     LLPPPGMAMERIYRIAPDNTTTGTPFTLAAATPGTARNATWYRYDAAGAAWQPIETTL
                     ANGTATVSVKAFGTYGVAAPLPASFTVGSNPAGARILLDGAYLGRVTPATISPIEPGN
                     HTLRLELEQFTPYEADITVPDNTTLFADLDGLARLKFDEPQREPGLDRTGGVYVMSRP
                     SGSAISIDGKRMTLTTPRVILGIREGSHTVQVVKEGQSFDTPTKAVWVEKDCIVPVMI
                     DAQDRAIVRKVSLESDACKGQGCTLNGRGPVFRLPRTVEVAGGSAFVTVQDGDCYISH
                     RITVTEAGETVRIAPRDPAFCGLLVESNPAGAEILIDGFRTGFCTPYLIRNVSDGPHV
                     VTVTRPGYLPGGEEILLTDDRNADIDKHLTFRLEAYPYGSLNVTSEPAGGEIYLYNKN
                     TGEVTPHTFRYMPIGTYEVKVVGNATFRTADDVTVRPYEVTKRAFDLI"
     assembly_gap    3009..3108
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3109..4061)
                     /locus_tag="PLI76_01190"
     CDS             complement(<3109..4061)
                     /locus_tag="PLI76_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOI60673.1"
                     /translation="MRECTPIWNSTLQKQTAIILLLLIAAPAAAGDFGGITLPDYKHI
                     DIQMANGGSKYVKFDGGGLNALHITTDPTDHYGQVTETDSQSGTFYFSDTGGRGFFDD
                     LILMVAVQGEVPDNFKVHIKASGYQWEPIPVLNKPPTMDEATHNEGAIDRNFTKDDFI
                     YAPQTWRPCKENYPIYYGQDMSKNNPEDRFYIMFIDLGIGAIGPNSFLDGLTDEGCAK
                     IEYSFENMETFAAFNAYGWCNQSNQGQGISWSNDLTGFAETGVSGYSVKGTKKSGDTG
                     GSGGIGSGAGSSGTSGIQGSNLNTSTTGSINGTLLAFTAPGP"
     gene            complement(4318..6681)
                     /locus_tag="PLI76_01195"
     CDS             complement(4318..6681)
                     /locus_tag="PLI76_01195"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOI60674.1"
                     /translation="MAFALIALPAAADSGIQLSATEPVAEISDDPGKESASVPEHAPG
                     ETPYATSAGQLTGSAPLPAPRHIFFNVANDAGVKHNLDGAAYGGPNGTYYIKADGGGL
                     NELHITNDAAAPAGQVTAGTDPSGVFWVANTGGRGYHDNVILLVSVNGTIPDDFALHI
                     RSSGYVWAPAPPGEYSPELPSEYQYADGAVDELFSRDDFIYGPQAWKPGPGDLEVPSL
                     PLYYGQEIDDPSAASHLIFVDLNVGNLKNGVYPGAALTDNGGAKVEFAFTNLSTHAAF
                     NAYGWCSASNQGQGISWTNRVAGAESSGYTVAGVPPAPPVAAFSANVTAGDAPLTVRF
                     TDLSTGNPTSWAWDFEDDGVIDSVEQNATFTYAAAGTYTVNLTVANAGGGDSAVKTDL
                     ITVTPPPGPTVLPGYNNIFIRVANNAGAKYNAFCNNTYNVMFEGIGRGLNALHVSTDP
                     AANFGQVTVTENQTGTFYATDSGGKGYEDDILLMVAVNGTIPDNFRLRITADGYTWTP
                     NPERNRAPSLDNVAYQPLSLDETFTKADFIYGPQTWKPTGNEADYPLHAGQDMNDAEN
                     TFFLMFIDLNAGVLRPNEALVNRGAVRINYDLDNLESFAAFSVYGYCRNPNNGDDVIA
                     WTNALSSDKVMSGYSVIGNAGKPVAAFSADRTGGWAPLSVAFSDLSAGQPTAWSWSFG
                     DGATSTGQHPVHTYEAAGNYAVRLAVTNGKGSDTLVKQGYITVSNVKGDFNGDGTVDI
                     GDVAKVACMVVGKTGTDPAADFNGNGAVDIGDAAKIACYFVGKIATL"
     gene            complement(6940..11163)
                     /locus_tag="PLI76_01200"
     CDS             complement(6940..11163)
                     /locus_tag="PLI76_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOI60675.1"
                     /translation="MTGDKRIQNGPWQGRIAVLLLILAIIAVPAVALGADLASNTTEP
                     TVGIPDEISPSVDVEDNGTPEPTLTEMVEEEVTLAAIEQLPQYIDIDTTPGIYAVIPS
                     NNNVLLQISNDGNARFNDYGNNTYHFFSPDQNAGQGMNALHITIDPAESSGQVTFSSD
                     QAGVFYLTDTGGRGWDDDGILMLAVNGTIPDSFRVNIKASGYQWTPVPENAKPAFDDI
                     IYVSEALNETFTKDDFLYGPQIWRPCAAANYPIFDGQDMADTENTFSILFIDLNAGIL
                     GVNTLSQPSFSGQSVTDNGAIKVEYSFENLPTLAAFDAYAYTVSSNQGQGIRWTNCLS
                     TSGSSGYAVIGQPLPEAPIADFTASATSGAVPFFVRFNDTSTNSPTAWEWDFGDGATS
                     TAQNATHTYMAAGTYSVNLTVTNEAGTDSKIKQDYITVGETPSGKGLADTAWPKFGYD
                     LQNTGQSPYLGPQTATIKWTYTISIEDPAGQPAIGSDGTIYIECIDRHLYALNPDGTP
                     KWNFTAGDRLYGSPAIGKDGTIYIGSTDKNLYAINPDGTLKWTYTTDSSISESSATIA
                     PDGTIYIGTTGYQKNTLHAISDDGTQGTTKWTFNAGQLYSTPAVGTDGTIYIGSLDKN
                     LYALNPDGTLKWTYTTGGKMQRTSPSIGTDGTIYIGNADNNLYAINPDGTLKWTYTFG
                     SYIYSSVAIGSDGTIYTGNNDKKVYAINPDGTLKWDFTTENMIRSTPVLGSDGTIYIG
                     SYDRKFYALNPDGTLNWMYTTGGGINCHAAIGSDGTLYIGTTENIIYAFKDPVTPPTA
                     DFTANVTSGDAPLAVAFTDTSTGAITSWAWDFGDAATSTEQNPTYTYTTPGTYTVNLT
                     VSNEAGSNSMVKTGYITVTELYVPQPARIVVTPPAAELAVGATQAFAATVYDTEERTM
                     DDAAVTWASSNETVGMVNTAGLFTARAPGTTTVTAAYANVAGTAVATVTLPKGDQTQD
                     TPLDIPGCNVTPGDDGKSQVSINTTTTNATVNGTTIRIEEDNFTLTIETEGAPTNESG
                     TVNGTVASIRLDTNPIVTDLASVGTVNASVSLNLTGLPQGAGLTTTVSQNVSADARSA
                     FQLAASAGGLALGDVAYTLNIVRTNLENGQDIAGATIRMAVSPAWVAAHGGVDAIRII
                     RLAEDGTKEVLATTLVGTDADGNMIFEAASPKGLSAFGLAAVSAASQPAGSTSSGGGG
                     SSTAVGAASNLKAGSSATLPMQMTAVSAVTLTAKTDIKDAMVTMAKGSLPQAAQPPAG
                     VVYQYVQATLYKAAEADLAAVQFRFAVPTAWLAEQGCTAGQIALLQYTADGWKNVPVE
                     ALGEENGNAVFTANPDAFGLFAVAATGEAPGTTGDVTPGITSSVTPTETKDTVAHAEG
                     TTATPKATPLPVWAAVLALGGALLLVRRP"
     gene            complement(11542..14181)
                     /locus_tag="PLI76_01205"
     CDS             complement(11542..14181)
                     /locus_tag="PLI76_01205"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HOI60676.1"
                     /translation="MEDSCSRTGRLQEWVIAMLLILVLGTVPAAALGTDLPSNATGSA
                     GMPEVSQIDVTPATAEATPGSMQRCIATAYDVSGSAISGMVFSWACSNESVGTVNETG
                     YFTALAPGSAVISAGTPDSMVLGTADVTVTSLLPELISAATTEPHTWIVDASGGGDYT
                     TIGAAVAVAGPSDTIIVRKGSYKENVVVNSSLTIRSESGPAETIITSANKDLPVFSLS
                     ANESTIEGLTIKGINGASSVITLTGAVGCLLENNIVTKSKFGIILEDTRENRITNNTF
                     YSFYDTCFVIRGADSDLLSGNTISSSEEGIIVYGASDRNSYHVTILGNTLINCDYAIY
                     LTGNPEKTKIINNILTECDYAIYLSGSSVGTEITNNTMTNGKYGLYMLNSPYGSTIIG
                     NTIQYQSIYGGILLQSTYGNVFYLNRIQNNTGDIAISSASATWNSTEPVTYVFENVTR
                     TGYVGNYWDRYVGSDADGDGIGDEPYVVSGTQKDYYPIVKPSNRIVTVTPDNVTLTAG
                     DGLQFTAVLSDREGTEIPDAGFTWTSSDEAVGTVNTTGYFSALKAGTATVGATSDGVA
                     GTAHVSVQRAETPAGGVIPAVDDVHLKVANDAGVRFNDFGDGSFRIRWSGGGQNAQHI
                     SNGSGSEYGEVTETTDLSGTFYATNTGGRGYFDNVFLCVAVNGTIPADFRLHIRADGY
                     QWTPLPICRDVPTPENRTYVNPTVDEWFTKDDLIYGPQTWRPAAGTTYPIYPGQDVSD
                     ESNQFRIMFVDLKTGVLHGYPIRVNYTIENLQTMAAFNVYGYSQNVDEVNDYLTTWTN
                     RLIGNTGEFSGWYVYGTPPESVKGDFDGNGIVDIGDVSKVAYMVVGKTAADPAADFNQ
                     NGKVDIGDAAKIACYFVGKIGEL"
     gene            complement(14573..15514)
                     /locus_tag="PLI76_01210"
     CDS             complement(14573..15514)
                     /locus_tag="PLI76_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60677.1"
                     /translation="MMTLMRGVDSGRLLMIVSHLFRPASVGSSPAVMRRGYTQGKEMI
                     DMQNNGKTMRLGAMTASAILLMLAVCIVPVSAADDVFTDNNQTYAAVANYDGARFDIF
                     GNNTYYFNFSKPTGGLNAIHITDSPSSLDGAVYTNRPASGTLYISDTSTDTQYHDDVI
                     LLFAVPNSTEHSNLELTLSVSGYQWVPTGSGNKPVLNGSYYNLSTLSETFENTDFLPG
                     TSSWRPAPLDDYPIYYGQGANEQFKIMLIDLYAGSVNQTNLENSGTVMVEYDLTGYEG
                     AAYFDTYAWNNQSAQGQGISWTNKIIGAGSSGWAIST"
     gene            16063..17058
                     /locus_tag="PLI76_01215"
     CDS             16063..17058
                     /locus_tag="PLI76_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOI60678.1"
                     /translation="MAGTDTEIAALNRRAREHAAALDAVRSGMETVIIGQQAVIDQVL
                     AAVLAGGHALLEGVPGIAKTLTISTLAHCLDCTFARIQFTPDLLPADITGTRIYDRNT
                     AAFRTIQGPIFHQIILADEINRAPPKVQSALLEAMQEGQVTIQGETYALPRPFFVLAT
                     QNPIESEGTYPLPEAQLDRFMLKIRMDYPTLDEEVAILDRFTTDQPESPAPVLDAAGL
                     VALRSFVRSVYADPGVKRYAAQLVDATRHPAAYGLDLGPMIASGASPRASLALLLCGK
                     AHAVLAGRGYVVPDDIRALAPAVLRHRVLLTFEAEADDLTPEEIVRRVLATVEVP"
     gene            17061..17948
                     /locus_tag="PLI76_01220"
     CDS             17061..17948
                     /locus_tag="PLI76_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOI60679.1"
                     /translation="MSDATERADLIRQIRRIELKTATLADGSCLGQRRSPFRGSGMAL
                     ANLREYLPGDDARAIDWKASARMDRLVVREFEEERSQTYYLVLDRSGSATFGSETSKD
                     RRTLEVAASLIFAAHRQNGRVGLCIVTDRVEVFLPARPGKSQVIRLVQALVSHRPEGS
                     GTDLAAAFRLLCTRVRRKSSVIVISDFDDPGFARDLSLLRRRHEATAVRVTDPAEDAL
                     PDVGQVFLEDPETGEQVLVDTSDPAFRKAYADAVAAADRRTGGDLRSCRVPEVRVSTD
                     EPYDRPLRRFYAHTRYRGG"
     gene            17949..18878
                     /locus_tag="PLI76_01225"
     CDS             17949..18878
                     /locus_tag="PLI76_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOI60680.1"
                     /translation="MTGFAEPLWLVGLAGIPVLCLLYRRAVRERSYAALTFSRVAVAA
                     QVRSPGRLPRPHALFALALLALALILVGLADPHVPIEGMDEGVSVVLVLDTSGSMQAT
                     DYRPTRLEAAKAAAETLLSRLSRDDYAGVVIFESGATSAAYLSPDRDRVIERLRGIRG
                     RDGQTALGDGLALGVDMADAIPNRHRVVILLSDGAGNAGVFSAEEAAGYARERNVPVY
                     TVGIGSANPPAGTQEYAALDEETLRAIARTTGGRYYAAVDEGTLAAVYAGLTDEIPRE
                     PKETSIRDLFFAGALGVLLLEVYLRYGRGRILP"
     gene            18875..20311
                     /locus_tag="PLI76_01230"
     CDS             18875..20311
                     /locus_tag="PLI76_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60681.1"
                     /translation="MRRLLLLPLLIAAVCVASVAAAGITLSTGQSDYAVLVGEEAAFP
                     IAVENTFGADVTGVLARNVRFVPEGGEAATHSQSRAITLFSDTREITVSAGPTVMPGT
                     FFFDIAFEYGNAPRTRVVLPEIRVHVAGSAAEEEGEQGPQVSSASTEAGSGGAQGGTS
                     STRQPGQIMQDTGSLREQAAREQDRMQALRSGLLAGLDDDPPVREIHAVLTSRGYTRV
                     AATINPVDEATGSFSFEYRNGAGDTVRAEGAVVNGSAAYAAYATEKSLVVPDAVTANE
                     TYKRYAGELADAGFFPATSSMNATTSASSVGVGFIDGQNRTARIVATLSGGEVRSVDI
                     EEPSDLLPLAGAGALCIILLIAYLVRRGAPKAPEPPVTPIPADPRGEALRLLAAARDE
                     FDRGERKGAYGTAGRALRSYVSSVYGTGAELADGDVLRLLEESGRETGAASSLLGRCA
                     AVSYAGSPPLDEGFRAVVGEIEAFIRRD"
     gene            complement(20298..20456)
                     /locus_tag="PLI76_01235"
     CDS             complement(20298..20456)
                     /locus_tag="PLI76_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60682.1"
                     /translation="MVMIRLFPDKPGRMKIKKMLKQGAYQPGFVNPVPDNDIGLTLSP
                     ERRRINLA"
     gene            20449..21153
                     /locus_tag="PLI76_01240"
     CDS             20449..21153
                     /locus_tag="PLI76_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOI60683.1"
                     /translation="MTILLALDDTDNLESRGTGRLARAVADAIAMHYPVWGVTRHQLY
                     VHPSIPYTSHNSCAVIHIVADGAEPLDTSLAIAEETMLGDFVEGSDPGLAGAPLDRVA
                     PALMAFGRDAQQMVLTQERARTLAKNLGIWLKGLGGTEDGVIGAMAGLGLAATYNDGR
                     FLQKGAIRSHTGPTPASTLLGAGIDEIMTLDGRKVTEGTIVLQEGKAAKACPINGRAV
                     LFVDDRDGELVAVKRN"
     gene            21156..21950
                     /locus_tag="PLI76_01245"
     CDS             21156..21950
                     /locus_tag="PLI76_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60684.1"
                     /translation="MEFWRRVLLAVGVLVVFVAVAFLVDTTLSQAYARESAFADSLPH
                     TAAIDRYDLRYGPQSWRPYATPIAIGPGDLPSGDWYIAFVELGVVPVGGNPALARTGS
                     VRVDYRFTDLAGTASLGVYGLHAGDGRAWTNRQSGYGTSGYYVSGTAAPGDLPRWTTP
                     LEAGNRYVVEVAGSPRPVDEDVARDTCTIWFERISSGLDGIHITTDRAAPKGQVTETA
                     DLDGSFYITYTGGNRVGALFLMVAVDRPQPEGFGLALESTFVEISR"
     gene            21947..22504
                     /locus_tag="PLI76_01250"
     CDS             21947..22504
                     /locus_tag="PLI76_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOI60685.1"
                     /translation="MMKRPSYFSLYEIALLSLFGALVFVLRTAIIVPIHVPGSSGIFW
                     VLPLIVGTAIVRKPGAGTYAGLVSGILASFFGIEPLHVFDVFKYTAMGITIDLVSMAF
                     GYRLENPVVGFVAGAAGNVVKMVVNYAVHLLLGVQGVFILLGIGLSSATHIVFGGIGG
                     IIAALIVGRLYRAGIVHQDDVPDDG"
     gene            22494..23927
                     /locus_tag="PLI76_01255"
     CDS             22494..23927
                     /locus_tag="PLI76_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI60686.1"
                     /translation="MTADPAIEFRSVSFRHPASRANRPREVLAGLDCVIGEGERVLLT
                     GPSGSGKTTFLRCCNGLIPHARPGTFKGDVFVRGRNTRDCTIGDLATTVGMVFQDPDH
                     QIFSNDVISEIAFGPEQLGWPPSRIDRAVNEILEQFRLDRLCDRQTTELSWGERQRLA
                     IASVVVLRPAILILDEPFSGLDAANAAGLLDLLERLNRESGMTIVLSEHRTEMVARWA
                     SRRISLLDGRLIEPPVPDAPLRDRPPRPGAKAARPGVVTLENVAFRHPGKVSPAMDGV
                     SLTLYAGEATVLAGPNGSGKSTLLRLLNGLLRPESGRIVVNGRDVRESTVAEIARTVG
                     LVCQHADYQLFGETIAEELAFAPANLGTPRDVIEQRVERAREGLGIEHLGLTASPLAL
                     SVGEKQRVSIASVLTMDTQVLALDEPTLGLDPAAKQRLAGLIDALCAHGKAVVIATHD
                     AGFSAAVADRIVRLERGRIVDDRRVQA"
     gene            23924..24739
                     /locus_tag="PLI76_01260"
     CDS             23924..24739
                     /locus_tag="PLI76_01260"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOI60687.1"
                     /translation="MTDGEGQRDRRHAYRAVGYIPADTFLHRMDPRTKLLTVLAISIA
                     AFATISPLRTAILFAFVVCLAVAAGLVRPFSRGLLLLLPLLVFIVVIDIFFPEISSGH
                     VYLSAQVGPFSSVITSGGLLYAAAKGIRVLVIGGISFLFVMTTSYTDFVASLRISRLP
                     PVIAFSLGYALKSMTALADDLRNIMDAQRSRALEFDRGTLIKNRRKLMALGVPMTVSI
                     LNRARYVTEAMQSRGFASTPRPTCYRPPRPGRHDLLLLVALGIVCLATLLIPV"
     gene            complement(24837..25253)
                     /locus_tag="PLI76_01265"
     CDS             complement(24837..25253)
                     /locus_tag="PLI76_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOI60688.1"
                     /translation="MRFIDANVFIYAILKPKAALPDTVLRKKAAAKDIFLRVNAGEPV
                     TTTTVHLSEVANVLEDAAGVAFATDLLSAILLKPAITVESVSADDYRESIHLARNHAI
                     GINDALAVVVMEREGISEIYTFDRHFRETRVKVVQE"
     gene            complement(25240..25476)
                     /locus_tag="PLI76_01270"
     CDS             complement(25240..25476)
                     /locus_tag="PLI76_01270"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOI60689.1"
                     /translation="MSKVEIRPVDAQGRIILPRSWRNRFKTGYVVIVAEDDRLEIRPA
                     DADLSRFVDSVEVEAEHFDDYHDLRKELRKNAVH"
     gene            complement(25553..26428)
                     /locus_tag="PLI76_01275"
     CDS             complement(25553..26428)
                     /locus_tag="PLI76_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOI60690.1"
                     /translation="MMKDYTLHTRGGVITERNADYATVRLRIPAGVLTADQLRQIARI
                     SVKYGDGSVHLTMRQTAEIPHVNPENLAKVAKALEKNGTPIGAEQNEVVNIMACPGTE
                     RCKYANCETIDLARKIDARVFGKELPIRLRIAISGCTYMCNSPLLNDIGIIGRIRPLR
                     IPGLCTGCGTCVEYCKQCAISLKNGISVLDEEKCVQCGVCVHSCPYHLLKSEYDHYQI
                     TVGGWRGTTPRAGRELVTVETEEEVVEVVDRIVYWVYRTAWSGRPLADQMDEIGFEKF
                     AEGIRKEFGEKAAEA"
     gene            complement(26466..27176)
                     /gene="cobS"
                     /locus_tag="PLI76_01280"
     CDS             complement(26466..27176)
                     /gene="cobS"
                     /locus_tag="PLI76_01280"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HOI60691.1"
                     /translation="MKSVLALLQFCTALPLGKPVEFEHFARRSYIYPLAGYVIGGIAA
                     GVVAWIGSPAVGAAVALAVVLVLSGCNHFDGLLDFGDGLMAHGSREKRIAAMTDRTTG
                     AGAVAAGIIVTLVAFAGLQDTAPLWAAILTAEVSAKVAMSYLTTLGVPFREGIHSYLH
                     GFARPWFVVPATLLALPLFLLPVSPMKIALALVAAAGIAAALLALSRHLFGGVNGDVV
                     GAANEITRAGVILLLALL"
     gene            complement(27173..27607)
                     /locus_tag="PLI76_01285"
     CDS             complement(27173..27607)
                     /locus_tag="PLI76_01285"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HOI60692.1"
                     /translation="MFEIEHRLEGKGIMRRDIVAAAMDLYVSHGIGADEAAKKLDEKI
                     GKAFEDPNVSSLLLSAILLEDELYWRRKNSEIADDPVFLLSDEIIGMAIAEVIGGTYA
                     RFEFTRYDQKKPGILSKLGPFLDDAVAGLIAGCTSRLYSESM"
     gene            27727..28743
                     /locus_tag="PLI76_01290"
     CDS             27727..28743
                     /locus_tag="PLI76_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00303 family protein"
                     /protein_id="HOI60693.1"
                     /translation="MSRPFLSKDPGLRPRRPVMALVLANTMLSTVPGISGAGPTPEKT
                     LLTPVLDAELVTTGAITSVPARPNTPTGCPTPATITRSMVELTGLSPVFINAGLVHTP
                     TVPCLDVYGSAGGDPRKGDAVPQAALLFERGETVGRLLAQYSDLLVLGECVPGGTTTA
                     LCVLRALGYDASVSSAFVENPLALKDAVCRDTLARIRKAGESEPLGILRAAGDPMMPV
                     AAGIASAYTGDILLAGGTQMLAVAATLKELGRRVPHLATTVYIRDDPSAGFSRSIADI
                     GTTAYYVDPDFAGLGHAGLARYCTGEVKEGTGAGGALMLAYLMGHKSEAITQKVVDFV
                     RKYV"
     gene            28818..29360
                     /locus_tag="PLI76_01295"
     CDS             28818..29360
                     /locus_tag="PLI76_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOI60694.1"
                     /translation="MLGIDDVADRVGSVGFRCRECGACCRRVAEDSNLVIVSPAEVRA
                     IMAATGMTWNEVAAPYPEFIDAGNGGEYTLAWCIRRTNEACIFLRDGRCSVYAHRPWI
                     CRTYPFMLVDDDLRVSECPGLGTPLSPRETRTLGADLCRRQAAEAAEEAGVRAVYREA
                     TIPPGGRAVIDSEGVKEVHG"
     gene            29353..>29581
                     /locus_tag="PLI76_01300"
     CDS             29353..>29581
                     /locus_tag="PLI76_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HOI60695.1"
                     /translation="MAEIHLVGTAHVSQKSIEDVRSAIEGFEPDIVGVELDRGRYVAL
                     TEEIAEPSIADILKGGNFGRLLVQWVLTYIQQ"
BASE COUNT         5042 a         9152 c         9345 g         5942 t
ORIGIN      
        1 ataggcctgt cagctcgaac agcctgctta tgcgagtaac ttatacgtat gacgttctct
       61 atataaagca tttctatcca cgccaaaagc gtgcattccg ggaaatcccc cgggaatcga
      121 cagaatctta ttctccggaa atccacgttc ttcgtatcat gaagacgctt gagtttcacc
      181 gcgacgacgc gaagggccgg gtgacggttg tgtgcgcaga tcgtgaggtg tcggtctact
      241 cctattgcgg ttactgccgg cactgtgcgg gtgtccgggt gggaaagcgg atgatcccga
      301 cgccgcaacg ccaggctctc tccggtctcc ggcagagtgc aaacccggat gagaacctgc
      361 taaacgccgc catcatgttt aacaccctcg tccgggacgg cagtgcgatc gagtgcgagg
      421 acgacaaggg cgaagggttc agttcgatgt accggcgctg aataccgatc tctgtcctcg
      481 ttttgccgcg cctctatcgg cacgacggca gggccccctc cttcgcgcag gacgggcggg
      541 gtgccgccca cggaagagcg ccggtggtcc ttgccccgca acgatccacc ggcgcccctc
      601 ccgcgcgagg ctgagaaaaa aaataggtgc gtgttccggt cagatcaggt cgaacgcacg
      661 tttggtcacc tcgtacggcc ggacggtgac gtcgtctgcc gtccggaacg tagcgttccc
      721 gacgaccttc acctcgtacg tcccgatcgg catgtaccgg aacgtatgcg gcgtcacctc
      781 gccggtgttc ttgttgtaga ggtagatctc tccacccgcc ggctcgctcg tcacgttcag
      841 gctgccgtag gggtaggcct cgaggcggaa cgtgaggtgt ttgtcgatat cggcgttccg
      901 gtcgtcggtg agcaggatct cctctcctcc gggcaggtat ccgggccggg tgacggtgac
      961 gacgtgcggc ccgtccgaga cgttcctgat gaggtagggc gtgcagaagc cggtcctgaa
     1021 gccgtcgatc aggatctcgg cgcccgccgg gttcgactcg acgagaaggc cgcagaaagc
     1081 ggggtcacgg ggcgcgatcc tgaccgtctc ccccgcctcg gtcacggtga tccggtgaga
     1141 gatgtagcag tcgccgtcct ggaccgtgac gaaggcgctc cctccggcca cctcgaccgt
     1201 ccggggcaga cggaagaccg gccccctgcc gttcagggta cacccctggc ctttgcaggc
     1261 atcggactcg agggatactt tccggacgat ggcccggtcc tgcgcatcga tcatgacggg
     1321 gacgatgcag tccttctcca cccagaccgc ctttgtcggg gtgtcaaacg actggccttc
     1381 cttgaccacc tgcaccgtat ggctcccttc ccggatgccg aggataacgc ggggcgtcgt
     1441 gagcgtcatc ctcttcccgt cgatggagat ggcggatccc gacggccggg acatgacgta
     1501 gacgccgccg gtccggtcca gccccggctc ccgttgcggt tcgtcgaact tcaggcgggc
     1561 aaggccgtcg aggtccgcga agagtgttgt attgtccggg accgtgatat cggcctcata
     1621 cggggtgaac tgttccagtt ccagccggag ggtgtggttc ccgggctcga tggggctgat
     1681 cgtcgccggc gtcaccctgc cgaggtatgc gccgtcgagg agtatccgcg cgccggccgg
     1741 gtttgagccg acggtgaagc tcgcgggcag gggcgcggcc accccgtagg ttccgaatgc
     1801 ctttacggat acagtcgccg tcccgttcgc gagggtggtc tcgatgggct gccaggccgc
     1861 tcccgcggca tcatagcggt accacgtcgc gttccgggcc gttccggggg ttgccgccgc
     1921 cagggtgaac ggggttcccg tcgtcgtgtt atcgggggcg atccggtata tccgctccat
     1981 cgccattccc gggggcggga ggaggccccg caccgcgtgg accgtcacct ccgccaccgc
     2041 accgcccgtg ctgttcgtcg cccggcaccc ggccgggaat gtgaggacca ggctcccgtc
     2101 ttctgcagtg tagcggacct cttcttccgg cgcgagggca aacgacccga tctcctcctc
     2161 gggggctgcc cctgcgccgg aggacacctc gatgccgggg gacgcgctct ccgcttcata
     2221 ccggacgacc agtatggcgt tccggttctg gaggtggtcg ccggcggcgc cgatgggata
     2281 actctgcacc tccacggtgt tgtcggatgc ctggatgaag gggagggcgt caagggttgc
     2341 ggtccagacg ccggccggcc cttcctggag ggggttgagc cagtcccagg cgttcagcgc
     2401 cacccggttc cccgtgccgc cctggccgga cccggcggtg ctcacgacgt agaggtgcgc
     2461 tgcggatgcc cgggcgcggt cgatggcgcc gtcgaacttc acctcggtcg tggcatcgtc
     2521 ggcggtcgtc ccctcccaga ggtccgcgag gacgtcgtca cagccgtccg ctatccagta
     2581 gtcgatccgg ggttttccgg gatcggcggc gacgagcacc aggagggcgg caggtatgcg
     2641 gcaggtggcc ccgggctccg catccatctg caccgtcagg gtgtggtcgc cggcggcgag
     2701 catctcgggc gggattgcga gggaaaacgt cccggccatc ggggccgaac cggtcccctg
     2761 caggtcgcgg gttacggcag gcatgaccgg cttgccgtcg aacctgaacg tcggcgtgat
     2821 atcggcgccg gccgccgcct cttttgcatc ggtcacgtag acgaagagga ggccgcggtc
     2881 gaccgtggcg ttctcgggga tggcgaccgg gaaccggagc tccgccgtcg cgcctgccgc
     2941 caggggttcc gccggcccgg gtgcggtgaa ggcgaggagg gtgccgttga tcgaaccggt
     3001 cgtcgatgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg cccgggtgcg
     3121 gtgaaggcga ggagggtgcc gttgatcgaa ccggtcgtcg atgtgttcag gttgctgccc
     3181 tgtatcccgg aggtgccgga cgagccggca ccgctcccaa tgcccccgga tcctcccgta
     3241 tcgccgcttt ttttggtgcc tttaaccgag tagccgctca cgccggtctc tgcaaaaccg
     3301 gtgaggtcgt ttgaccacga tatcccctgt ccctggttgg actggttgca ccaaccgtag
     3361 gcgttgaacg ccgcgaacgt ctccatgttc tcaaaggaat actcgatctt tgcacacccc
     3421 tcgtcggtca gcccgtcaag gaaactgttc gggccgattg caccgatacc gagatcgatg
     3481 aacatgatgt aaaaccggtc ttcggggttg ttcttgctca tatcctggcc gtaatagatg
     3541 ggataattct ccttgcaggg tctccaggtc tgcggcgcgt agatgaagtc gtctttggtg
     3601 aagttccggt cgatcgcgcc ttcgttatgc gtggcctcgt ccatcgtcgg aggcttgttc
     3661 aggaccggga taggctccca ttggtagccg cttgccttga tatggacttt aaagttgtcg
     3721 gggacctctc cctgcaccgc caccatcagg atgaggtcgt cgaagaaccc ccggccgcct
     3781 gtgtcggaga agtagaaggt gcccgactga ctgtctgtct ccgtcacctg cccgtaatga
     3841 tcggttgggt cggtcgttat gtggagggcg ttgagcccgc cgccgtcgaa cttgacgtac
     3901 ttacttccgc cgtttgccat ctggatgtcg atgtgtttgt agtcggggag ggtgatcccc
     3961 ccgaagtcgc ccgctgcggc cggtgcggcg atgaggagca gcaatatgat ggccgtctgc
     4021 ttctgcagcg tgctattcca aataggtgta cattctctca tgggattacc cgccgttctg
     4081 cgatggtgct ggaaaagcca actcaggatc tgcacccgga agcctgcatg tgcatggatc
     4141 ccttttcgct gatggggtaa ggtgcaacag cggagtataa gaccgttatt aatttggatg
     4201 ggtataaatg aatcatctta acatacttca tacggcgcta aaaataatga ttttttatta
     4261 ccggttcccg gtgggcacca tgccgatacc ccgccggcct gtccgtttcg tgcaggatca
     4321 tagcgtcgcg atcttcccga cgaagtagca ggcaatcttt gccgcgtccc cgatatcgac
     4381 cgcaccgttc ccgttgaagt ctgcggcagg atcggtcccc gtcttcccga cgaccatgca
     4441 ggcaaccttt gcgacatcgc cgatatcgac ggtcccatcc ccgttaaaat cgcctttcac
     4501 gttcgatacg gtgatataac cctgctttac gagggtgtcg gagcccttgc cgttggtgac
     4561 ggcaaggcgc accgcatagt tgcctgccgc ctcgtaggtg tggaccgggt gttgcccggt
     4621 cgaggttgct ccatcgccaa acgaccacga ccatgccgtg ggctggccgg ccgacaggtc
     4681 cgagaacgca acgcttaacg gagcccatcc gcccgttctg tcggccgaga acgccgccac
     4741 gggctttcct gcgttaccga tgaccgagta gccgctcatc accttatcgg aggagagggc
     4801 gtttgtccag gcgatcacat cgtcgccgtt gttggggttc ctgcagtagc cgtatacgct
     4861 gaaagcggca aacgattcca ggttatcgag gtcgtagttg atcctgaccg ctccccggtt
     4921 cacgagagcc tcgttcggcc ggagaacgcc ggcgttgagg tcgatgaaca tgaggaagaa
     4981 ggtgttctcc gcgtcattca tatcctggcc ggcatggagg ggataatccg cctcgtttcc
     5041 ggtcggtttc caggtctgcg ggccgtagat gaagtctgcc tttgtgaagg tctcgtcgag
     5101 ggagaggggt tggtacgcca cgttgtcgag ggagggcgcc cggttccgct cggggttcgg
     5161 ggtccaggta tacccgtccg ccgtgatgcg gagcctgaaa ttgtcgggga tggtgccgtt
     5221 gacggcgacc atgaggagga tatcgtcctc gtatcccttc ccgccggagt cggtggcata
     5281 gaaagtgccg gtctggttct cggtcaccgt cacctggccg aagtttgcgg ccggatcggt
     5341 ggagacatga agggcgttga ggccgcggcc gatcccttcg aacatgacgt tataggtatt
     5401 gttgcagaac gcgttgtact tcgccccggc gttgtttgcc acccttatga agatgttgtt
     5461 gtacccgggg aggacagtcg gcccgggagg cggcgtaacg gtgatgagat cggtcttgac
     5521 cgcggagtcg ccgccgccgg cgttcgctac ggtcaggttc acggtgtagg tgccggcggc
     5581 ggcatacgtg aacgtggcgt tctgctcaac gctatcaatg acgccgtcgt cctcgaagtc
     5641 ccaggcccag gatgtcgggt tgccggtgga gagatcggtg aaccgcaccg tgagcggagc
     5701 gtcgcccgcg gtgacgttgg ccgagaaggc cgcgaccggt ggagccggcg gcacgccggc
     5761 gacggtgtaa ccgctggatt cggctcccgc tacccggttc gtccaggata tcccctggcc
     5821 ctggtttgaa gcgctgcacc agccgtaggc attgaatgcc gcgtgggtgg agaggttggt
     5881 gaacgcaaac tcgaccttcg ccccgccgtt gtcggtcagg gcggccccgg ggtacacgcc
     5941 gttcttgagg tttccgacgt tcaggtcgac gaagatcaga tgggatgctg cggagggatc
     6001 gtcgatctcc tgcccgtagt agagcggcag ggacggtact tcgagatcgc ccgggccggg
     6061 tttccaggcc tgcgggccgt agatgaagtc gtccctggag aagagttcat ctacggcacc
     6121 gtcggcatac tggtactcgg acggcaactc cggcgaatat tcaccggggg gtgcaggcgc
     6181 ccagacatat ccgctcgaac ggatgtgcag ggcaaagtcg tcgggtatcg tcccgttcac
     6241 cgagaccagg aggatgacgt tgtcatggta tccccgcccg ccggtgttgg cgacccagaa
     6301 cacgccggac gggtcggtgc ccgcagtgac ctgccctgcg ggagcagccg cgtcgttcgt
     6361 gatatggagt tcgttcaggc cgccgccgtc cgccttgatg tagtaggtgc cgttcggccc
     6421 gccgtatgcg gcgccgtcca ggttgtgttt caccccggcg tcgtttgcga cgttaaagaa
     6481 gatgtgcctg ggtgccggga ggggtgcgga gccggtgagt tgcccggccg atgtcgcata
     6541 cggcgtctcg cctggagcat gctcgggtac ggaggcgctc tctttgccgg gatcgtccga
     6601 tatctccgca accggttctg tcgcactcaa ctgtatgccg ctgtccgctg ccgcaggcaa
     6661 ggcgataagg gcaaaggcca ataccagggc ggatatccgc ctccatgata cggtgttttg
     6721 agtatactca tcaattccca tgacggccgc tcctttcgcc aggatggttt ctgcattcgc
     6781 agggatgcca gccgagcaat gcaatcattc tggtttacgc tatatgagcg tttctaatcc
     6841 cgaagggatt aataaggttt gttttacgaa attctctgat ctgcaaagtc aatcggatga
     6901 atactcctga tgcccgcctg cggagaaaaa aaggtgtgat cagggtctcc tcacgaggag
     6961 gagagctccg ccgagtgcca ggaccgccgc ccagaccggg aggggcgtgg ccttcggcgt
     7021 cgccgtggtt ccctcggcgt gtgcgaccgt atctttcgtt tcggtcggtg tcacggacga
     7081 ggtgatcccg ggggtcacgt cgccggttgt gccgggagcc tctccggtcg cggcgacggc
     7141 aaagaggccg aacgcatcgg ggtttgcggt gaagacggcg ttgccgttct cctcaccgag
     7201 cgcctcgacc gggacgttct tccagccgtc ggcggtgtac tggaggaggg cgatctgtcc
     7261 tgcggtgcag ccctgctctg cgagccaggc ggtcggcacg gcgaaccgga actggacggc
     7321 ggcgaggtcc gcctcggccg ccttatagag ggtcgcctgg acgtactggt agaccacgcc
     7381 cgccgggggt tgtgcagcct gcgggagcga gcccttcgcc atggtcacca tggcgtcttt
     7441 gatgtcggtc ttcgcggtga gggtgaccgc cgagacggcc gtcatctgca tcgggagggt
     7501 ggcgctactg ccggccttga ggttgcttgc cgcgccgacc gcggtcgatg aaccgccgcc
     7561 gcctgacgag gtagacccgg cgggctgcga tgctgccgag actgcggcaa gcccgaaggc
     7621 cgagaggccc tttggcgatg cagcctcaaa gatcatgttc ccgtcggcgt cggtgccgac
     7681 gagcgtcgtc gcaagcactt ccttcgtgcc gtcctcggcg agccggatga tccggatcgc
     7741 gtccacgccg ccgtgggcgg cgacccaggc ggggctgacc gccatccgga tagtggcgcc
     7801 ggcgatgtcc tgtccgttct caaggttcgt cctcacgatg ttcagcgtgt aggcgacgtc
     7861 gccgagggca agcccgccgg cgcttgccgc gagctggaag gcgcttctcg catcggcgga
     7921 gacgttctgc gagacggtcg tcgtgaggcc ggctccctgc gggaggccgg tcaggttcag
     7981 cgagacggag gcgttgaccg tgccgaccga tgcgagatcc gtgacgatcg ggttcgtgtc
     8041 gagcctgatg ctcgcgactg ttccgttcac ggtgccgctc tcgttggtgg gagcaccctc
     8101 ggtctcgatg gtgagggtga agttgtcctc ctcaatccgg atggtggtcc cgttgacggt
     8161 tgcgttggtg gtggtggtgt tgatgctgac ctgcgacttg ccgtcgtcgc ccggggtcac
     8221 gttgcagccg gggatgtcga ggggagtgtc ctgggtctgg tcgcctttcg gcagggtcac
     8281 ggtggcgacg gcggtgcccg ctacgttggc gtacgcggcg gtgacggtcg tcgtgcccgg
     8341 ggcgcgggcg gtgaagaggc cggcggtgtt caccatgccg acggtctcgt tcgagctcgc
     8401 ccaggtgacg gcggcgtcat ccatcgtcct ctcttcggtg tcgtagacgg tcgcggcgaa
     8461 tgcctgcgtt gccccgacag cgagttccgc tgccggcggg gtgacgacga tgcgggccgg
     8521 ctgcggcacg tacagttcgg tgacagtgat gtaaccggtc ttgaccatgg agttgctgcc
     8581 ggcctcgttt gagaccgtca ggttgacggt gtaggtgccg ggagtggtgt aggtgtaggt
     8641 cgggttctgc tcggtcgagg tcgcggcgtc accaaagtcc caggcccagg atgtgatcgc
     8701 gcccgtggag gtatcggtga aggcgaccgc gagtggggcg tcgccggatg tcacgttggc
     8761 ggtgaagtcg gcggtgggcg gagtaacggg atccttaaat gcatagatga tattttccgt
     8821 ggttccaatg taaagggttc cgtcggatcc gattgccgca tggcaattaa tcccgccgcc
     8881 ggtggtgtac atccaattca gtgttccgtc cgggttcaat gcatagaatt ttctatcgta
     8941 gctgccgatg tagatggttc cgtcagaacc gagtaccggc gtactgcgga tcatgttttc
     9001 cgtggtaaaa tcccatttga gtgttccgtc agggtttatt gcatagactt ttttatcatt
     9061 atttcctgtg tagatggtgc cgtcggatcc gattgcaaca gagctgtaga tgtaggatcc
     9121 aaaggtgtag gtccatttga gcgttccgtc cggatttata gcgtagaggt tgttgtctgc
     9181 gttaccaata tagatagttc cgtctgtacc gatacttggg gaggtacgtt gcattttccc
     9241 cccggtggtg taggtccatt tgagcgttcc atccggattt aatgcataga ggttcttgtc
     9301 caaacttccg atgtagatgg ttccatctgt tcccacagcg ggcgtgctgt ataactgacc
     9361 tgcattgaac gtccatttag tagttccctg ggttccatcg tcggatattg catgtaaagt
     9421 atttttttga taaccagtgg taccgatgta gatggttccg tccggagcga ttgttgcgga
     9481 agattcggat attgaactat ctgttgtgta agtccatttg agcgtcccgt caggattgat
     9541 tgcgtagagg ttcttatcgg tgcttcctat atagatggtt ccgtcttttc ctatagcagg
     9601 tgatccgtat aacctatctc ctgcggtaaa attccatttg ggggttccat ctgggtttaa
     9661 tgcgtagaga tgtctgtcaa tgcattcaat atatatggtt ccgtccgacc cgatggcagg
     9721 ctgacccgct ggatcctcta tgctaatcgt gtaagtccat tttatagtgg ccgtctgcgg
     9781 gccgaggtag ggagactgcc cggtattctg gaggtcgtag ccgaacttcg gccaggcggt
     9841 atcggcaagg cctttcccgg acggggtttc gccgaccgtt atgtaatcct gttttatctt
     9901 gctgtcggtg cccgcctcgt tcgtcaccgt aaggttcacg gaatacgttc ccgcggccat
     9961 gtaggtgtgc gttgcatttt gagctgtcga ggtggctcca tcgccgaagt cccactccca
    10021 ggcagtcggg ctgttggttg acgtgtcgtt gaaacgcacg aagaacggca cagccccgct
    10081 ggttgcgctg gcggtgaagt ccgcgatggg ggcttccggg agcggctggc ctatgacggc
    10141 gtatccggac gacccgctgg tgctcaggca gttggtccag cggatgccct ggccctggtt
    10201 cgaggagacc gtgtaggcat acgcatcgaa ggccgcgagc gtcggcaggt tctcgaagga
    10261 gtactcgacc ttgatcgccc cgttatcggt gacggattgt cccgagaaac tcggctggct
    10321 gagcgtattc acgccaagga tgcctgcgtt caggtcgatg aacaggatcg agaaggtgtt
    10381 ctcggtatcc gccatgtcct gcccgtcgaa aatggggtag ttcgccgccg cgcagggcct
    10441 ccagatctgc ggtccgtaga ggaagtcgtc cttggtgaac gtctcgttca gggcttcgga
    10501 aacgtagatg atgtcatcga acgctggctt ggcgttctcg ggcactggcg tccactggta
    10561 gccgctcgcc ttgatgttga cccggaaact atcgggtatc gttccgttga cggcgagcat
    10621 cagaatgccg tcgtcgtccc agccgcggcc gccggtgtcg gtgaggtaga agacgcccgc
    10681 ctggtcgctg ctgaatgtga cctggcctga agattccgcc ggatcgattg taatatgaag
    10741 agcgttcatg ccctgaccgg cgttctggtc tgggctgaag aagtgataag tgttgttgcc
    10801 gtaatcgttg aaccgggcgt tcccgtcgtt tgagatctgc aacagcacgt tattgttcga
    10861 tggtattact gcgtagatgc cgggagtggt atcgatgtcg atatactgcg gcaactgctc
    10921 gatcgctgcc agcgtcacct cttcttcgac catttcggtc agtgttggtt cgggtgtgcc
    10981 attatcctcc acatcgacac tcggagatat ctcatcggga atcccgacag tcggttcggt
    11041 tgtgttcgac gccaggtcgg ctcccagcgc caccgccggt acggcgatga tcgcgaggat
    11101 cagcagcagg acggctatcc gcccctgcca tggaccgttc tgaatacgtt tatcccctgt
    11161 cataatactt ccctttcaaa tcgagaattc tcgaagaagt ttctgcggaa ccggcacccc
    11221 atgtgcctgc atcgctataa ccgctctctg agaatgagta aatttttaat cgagaggtat
    11281 aaggttgttg ctaaatcggg ctgttttaaa tgagtttagt taacaaattt gagttttcac
    11341 taaagataat gtatcttctg cagacgtggc atactcctcc tgcccacaaa gaaccggaga
    11401 gctaaagggt acgacaggtg gtggcgatga aaaaatagac gatctggagt caggtgcggc
    11461 cttcatctcc ggtgcgtctg ctccgccggc gtcgggatca gcaccgggcc gtattttgat
    11521 atggaatctc gcgggaaggg gttagagttc ccctatcttc ccgacgaagt agcaggcgat
    11581 ctttgccgca tccccgatat cgaccttccc gttctggtta aagtccgcgg ccgggtcggc
    11641 cgccgtcttc ccgacgacca tataggccac cttcgagacg tcgccgatat cgacgatgcc
    11701 gtttccgtcg aaatcgcctt ttacgctttc cggaggcgtg ccgtaaacat accaaccgct
    11761 gaactcgccg gtattcccga tcaggcggtt tgtccaggtg gtgaggtaat cgttcacctc
    11821 atccacgttc tgagagtacc cgtagacgtt gaaggccgcc atcgtctgga ggttctcgat
    11881 cgtgtaattc acccttatcg gatagccgtg gagaacgccg gtcttcaagt cgacgaacat
    11941 tatccggaac tggttcgatt cgtcgctgac atcctggccc ggatagatcg ggtacgtggt
    12001 acctgccgcc ggccgccagg tctgcggacc gtagatcagg tcgtccttcg tgaaccactc
    12061 gtcgaccgtc ggattcacgt aggtgcgatt ctcaggcgtg ggtacgtcgc gacaaatcgg
    12121 aagcggcgtc cactggtatc cgtcggcccg gatgtgcagg cggaagtcgg cggggatggt
    12181 accgttcacc gcaacacaca ggaagacatt gtcaaagtat cccctgccgc cggtgttggt
    12241 ggcatagaag gttccggata ggtcggtggt ctctgtcacc tctccgtatt cgctgccgga
    12301 cccgttcgag atatgctgag cgttctgtcc gccgccggac cagcggatgc ggaatgaacc
    12361 atcgccgaaa tcattgaacc ggacgcccgc atcgtttgcg accttgaggt gaacgtcgtc
    12421 gactgcgggg atgacgccgc ctgccggtgt ctccgcacgt tgcacgctca cgtgcgcggt
    12481 tcccgccacc ccatcgctcg tggcgccgac cgtcgccgtc ccggccttca gggccgagaa
    12541 atacccggtg gtattcaccg tgccgaccgc ctcatcgctg ctcgtccagg tgaacccggc
    12601 gtccgggatc tccgtgcctt cccggtcgga cagtacggcg gtgaactgca gaccgtcgcc
    12661 tgccgtcagg gtcacattat ccggggtgac ggtaacaatg cggttgcttg gtttgacgat
    12721 ggggtagtag tccttctggg tgccggacac gacatacggt tcgtcgccga tcccgtcgcc
    12781 gtcggcatcg gatccgacgt accggtccca gtaatttccg acgtaaccgg ttcgggtgac
    12841 gttctcgaag acgtaggtga ccggctccgt cgagttccag gtggcgctcg cactggatat
    12901 tgcaatatct ccggtattat tctgaatcct gttgaggtag aagacattac cgtaggtgct
    12961 ctgcagtaga atgccgccat agatagactg atattggatg gtgtttccga tgatagtgct
    13021 gccatatgga ctgttcagca tgtacagccc atattttcca tttgtcattg tgttgttagt
    13081 gatttcagta cctaccgaac taccgctgag atagatcgca tagtcgcatt cggtcaggat
    13141 attgttgatg attttggtct tttctggatt acctgtgagg taaatcgcgt agtcgcaatt
    13201 gatcagggta ttgccgagga ttgtcacatg gtacgaatta cgatcgcttg caccatagac
    13261 aataatccct tcctcggagg acgagatcgt atttcctgat agcagatccg aatcggcccc
    13321 tctaatgacg aaacaggtgt cgtagaacga atagaatgta ttgttagtga tccgattttc
    13381 acgggtgtcc tccagaatga tgccgaattt cgatttcgtg acaatattat tttcgagaag
    13441 gcatccgacg gctcctgtga gtgttatcac ggaagatgct ccgttgatcc ccttaatcgt
    13501 cagcccctca atggtcgact cgttggccga aagggagaac acgggtaggt ccttgttggc
    13561 agaagtgatg attgtctctg ccgggccgct ctccgaacga atcgtcagcg agctgttcac
    13621 aacgacgttt tccttgtaac ttcccttccg gacgatgatc gtatcggatg gtcctgcaac
    13681 ggctacggcg gcaccgatgg tcgtgtagtc ccctccaccg gaggcgtcga cgatccaggt
    13741 gtgcggctcc gtcgttgccg cagaaatcag ttcaggtaac agggaggtga cagtcacgtc
    13801 cgcggtcccg agcaccatac tgtctggggt gccggcactg attaccgcag acccgggtgc
    13861 aagcgccgtg aaatacccgg tctcgttcac tgttccgacg gattcgttgc tgcatgccca
    13921 ggaaaatacc atccctgaaa tcgcagaacc cgatacatcg tacgccgtgg caatacatcg
    13981 ttgcatgctg cccggagtcg cttcagccgt tgccggcgtc acatcgattt gggagacctc
    14041 tggcatcccg gctgatccgg ttgcgtttga cggcaggtcg gtacccaatg ccgccgccgg
    14101 cacggtgccg agcacgagga tcaacagcat ggcgatcacc cattcctgca atcttccggt
    14161 tcgactacaa ctatcttcca tgtcagacca atcctttcat tcagtaggtg taggaggaaa
    14221 tctacacaaa accggggatc gggtcgcctt tatttgcgta gaatctctca atccaatgtg
    14281 ttacgattta ataacgcatt ataagaagat actgaattta cgtggggtta atgagggtca
    14341 attaacgtga tctctctcct ttctcaagga gaacctttcc tgtcctgcag caccgccgta
    14401 tcgtcctgaa atatgatgga aaaaatcgtg cgacgggaca tatcggcagg atcgggctga
    14461 tcggggaatc cctccagagt agattcttga ccatccctac ttcacagggc atacacggtg
    14521 aaacaagagt cccttccgga gctcgaaaaa aaagggaggg tggttgttgt gttcaggtgc
    14581 tgatcgccca accgctgctc ccggctccga tgattttgtt cgtccaggaa attccctgcc
    14641 cctgagctga ctgattgttc catgcgtagg tatcgaagta agcagcgccc tcataccccg
    14701 taaggtcata ctcgaccatg accgtcccgc tgttctcaag gttggtctgg ttgaccgatc
    14761 ccgcgtacag gtcgatgagc atgatcttga actgctcgtt agcgccttga ccgtaatata
    14821 tcggatagtc atccaggggc gcaggtctcc acgacgatgt gccggggagg aaatcggtgt
    14881 tctcgaacgt ttcgctaagt gtcgatagat tgtaataact tccgttaagc actggcttgt
    14941 ttccgcttcc tgtaggcacc cactgataac cagagacgct caacgttagt tcgaggttag
    15001 agtgttcggt cgagttcggc acggcaaaca gcagtatcac atcgtcatgg tactgggtat
    15061 ccgtgctggt gtcggagatg tagagtgtgc ccgatgccgg ccggttggtg tatacggcgc
    15121 cgtcaagact cgatgggctg tcggtgatgt gtatcgcgtt cagtccgccg gtaggcttcg
    15181 agaagttgaa gtagtacgtg ttgttcccga atatgtcgaa tctcgctccg tcatagttcg
    15241 ccacggcggc atatgtctgg ttgttgtccg tgaagacatc gtcggccgcg gaaaccggca
    15301 cgatgcagac ggcaagcatc agcaggatcg ccgatgccgt catcgcaccg agtctcattg
    15361 tttttccatt gttttgcatg tcaatcatct ctttcccttg agtgtagccg cgccgcatga
    15421 cggccgggct actgccgaca gaagccggac gaaaaaggtg actaacgatc atgagcaaac
    15481 ggccgctgtc aacaccccgc attaaggtca tcatattata aatacttttc ttatatcgat
    15541 ttatgccgaa tattcatgtg atcaggccga taagatgaaa taatgtggag taattgggaa
    15601 aatatcggag acaatctata tctctaaagg caattattta tactatacaa cgatccgctg
    15661 gatgagagat cgcgcatacg ctgcgatgta ccggcacgtg gcggatgctt gcgatacggc
    15721 tccgcctttc ctgcctgcgg ggattttcgt agtgtgcaga atgatattgt cgaaggggca
    15781 tcgcaagccc gggtcagggc accggacggc ctcgcgtttt cagcgccgga tcgggtgtca
    15841 tcgcgccatc aggtacttgc cgataaatac gcccgtcacc gtaggaaaac cctatgatat
    15901 cctcgaaacc ggtagatcac tatggagtgg agtttttctg tggtactgcc gcaggagccc
    15961 tcccgtattc gaaaccggtt tcgttaatct gccctgatta acccatcccc aactaacaac
    16021 cgtcaaatag catctccccc aacctctctt ccaggaatca gcatggcagg cacggatacg
    16081 gagattgcag ccctcaaccg ccgggcacgg gagcacgcgg ccgcgctcga cgcggttcgc
    16141 tccggcatgg agacggtgat catcggacag caggcggtta tcgaccaggt gctcgcggcg
    16201 gtgcttgccg gcggccacgc cctcctcgaa ggggtgccgg ggatagcaaa gacgctcacg
    16261 atcagcaccc tcgcgcactg cctcgactgc accttcgccc ggatccagtt cacccccgac
    16321 ctcctccccg ccgacatcac cgggacccgg atctacgacc ggaacacggc cgccttccgg
    16381 acgatccagg ggccgatctt ccaccagatc atccttgccg atgagatcaa ccgtgccccc
    16441 ccgaaggtgc agtccgccct cctcgaggcg atgcaggagg ggcaggtcac catccagggc
    16501 gagacctacg cgctgccgcg cccgttcttc gtcctcgcca cccagaaccc gatcgagtcc
    16561 gagggcacct acccgctccc cgaggcgcag ctcgaccgat tcatgctcaa gatacggatg
    16621 gactacccga cactcgacga agaggtcgcc atactcgacc ggttcacgac ggaccagccc
    16681 gaatccccgg cgccggtcct cgatgccgcc ggtcttgtcg ccctccggtc gttcgtgcgg
    16741 tcggtctacg ccgacccggg cgtcaaacgc tacgccgcgc agctggtcga cgccacccgg
    16801 cacccggcgg catacggcct cgacctcggc cccatgatcg cctccggggc atccccgcgg
    16861 gcatcgctcg ccctcctcct ctgcggcaag gcgcacgcgg tgctcgccgg ccggggctac
    16921 gtcgtccccg acgacatccg ggccctcgcc cccgccgtcc tcaggcaccg ggtcctcctc
    16981 accttcgagg cggaggcgga cgatctcacc cccgaagaga tcgttcggag ggtcctcgcg
    17041 accgtggagg tgccctgacg gtgagcgacg ctacggagcg ggcggatctc atcaggcaga
    17101 tccggcggat agaactcaag accgccacac tggccgacgg ctcctgcctc ggccagaggc
    17161 gctccccctt ccgggggagc gggatggcgc ttgcaaacct ccgcgagtac ctccccggcg
    17221 acgacgcccg ggcgatcgac tggaaagcct cggcacggat ggaccggctc gtcgtcaggg
    17281 agttcgagga ggagcggagc cagacctact acctcgtcct cgaccggtcg gggtccgcga
    17341 cgttcgggtc agaaacgagc aaagaccgcc ggacactcga ggtggccgcg agcctcatat
    17401 ttgccgctca ccggcagaac gggcgggtgg gcctctgcat cgtcaccgac cgcgtcgagg
    17461 tcttcctgcc ggcccggccg gggaagagcc aggtcatccg gctcgtgcag gccctcgtct
    17521 cccaccggcc ggagggaagc ggcaccgacc tcgccgccgc gttccgtctc ctctgcacga
    17581 gggtccggcg caagtcctcg gtcatcgtca tatccgactt cgacgacccc gggttcgccc
    17641 gcgacctctc gctcctgcgc cggcggcacg aggcgactgc cgtcagggtg acggaccccg
    17701 cggaagacgc cctcccggac gtggggcagg tcttcctcga agaccccgag accggcgaac
    17761 aggtgctcgt ggacacgtcg gaccccgcct tccggaaggc ctacgccgac gccgtggccg
    17821 ccgccgaccg gcgcaccggg ggcgacctgc gctcgtgccg ggttcccgaa gtccgggtct
    17881 ccacggacga gccctacgac cggcccctgc gccgttttta cgcgcacacg cgctaccggg
    17941 gcggttagat gaccggattt gcagaaccgc tctggctcgt cggccttgcc ggcatcccgg
    18001 tcctctgcct cctctaccgg cgcgccgtcc gggagcggtc gtatgccgcg ctcaccttct
    18061 cccgcgtcgc cgtcgccgca caggtccgga gccccggccg gctcccgcgc ccgcacgccc
    18121 tcttcgccct tgccctcctc gcgctcgccc tcatcctggt cggcctggcc gacccgcacg
    18181 tcccgatcga ggggatggac gagggcgtca gcgtggtcct ggtgctggac acatccggga
    18241 gcatgcaggc gaccgactac cgccccaccc gcctcgaggc ggcaaaggcc gccgcggaga
    18301 ccctgctctc ccggctcagc agggacgact acgcgggggt cgtcatattc gagtcgggcg
    18361 cgacgagcgc tgcctacctc tcccccgacc gcgaccgggt gatcgagcgg ctccgcggca
    18421 tccgggggcg ggacggccag acggccctcg gcgacgggct tgccctcggc gtcgacatgg
    18481 ccgacgccat accgaaccgg caccgggtgg tgatcctcct ctccgacggc gccggcaatg
    18541 ccggcgtatt ctccgcggag gaggctgccg gctacgcccg cgagcggaac gtcccggtct
    18601 acaccgtggg catcggctcg gcaaacccgc cggcgggcac ccaggagtac gccgcgctcg
    18661 acgaagagac cctccgggcg atcgcccgga cgaccggggg aaggtactac gcggcggtcg
    18721 acgaggggac gcttgccgcg gtctatgccg gtctgaccga cgagatcccg cgcgagccaa
    18781 aagagacgag catccgcgat ctcttctttg ccggggcgct cggggtcctc ctccttgagg
    18841 tctacctccg ctacggtcgg gggaggatcc tcccgtgagg cgccttctcc tcctccccct
    18901 tctcatcgcc gccgtctgcg tggcctccgt cgcggccgcc ggcatcaccc tctcgaccgg
    18961 gcagtccgac tacgccgtgc tggtcgggga agaggccgcg tttcccatcg ccgtcgagaa
    19021 cacgttcgga gccgacgtca ccggagtgct cgcccggaac gtgcggttcg tgccggaggg
    19081 cggtgaggcg gccacccact cccagtcccg ggcgatcacc ctcttttcgg atacccggga
    19141 gataaccgtc agcgccgggc ccacggtaat gcccggcacg ttcttcttcg atatcgcgtt
    19201 cgagtacggg aacgccccgc gcacccgcgt cgtcctcccc gagatccggg tccacgtcgc
    19261 cggttcggcc gcagaggaag agggggagca gggcccgcag gtgagttccg cctcgacgga
    19321 ggcggggtcc gggggggcgc agggagggac ttcgtccacg cggcagcccg ggcagatcat
    19381 gcaggacacg ggttcgctcc gggagcaggc ggcacgggag caggaccgca tgcaggcgct
    19441 gcggtcaggt ctgcttgccg ggctcgatga cgacccgcct gtccgggaga tccacgccgt
    19501 cctcacgtcg cgcgggtata cccgggtggc ggcgacgatc aacccggtgg atgaggccac
    19561 gggatcgttc tcgtttgagt atcgcaacgg cgccggggat acggtgcgcg cggagggcgc
    19621 ggtggtgaac ggttctgcgg cctacgcggc gtatgccacc gaaaagtcgc tcgtggtgcc
    19681 ggatgcggtc acggcaaacg agacctacaa acggtatgcc ggggaacttg ccgacgccgg
    19741 tttcttcccg gcgacgtcat cgatgaacgc gacgacgagc gcctcctctg tcggcgtagg
    19801 gttcatcgac ggccagaacc ggacggcgag gatcgtcgcc acgctctccg ggggggaggt
    19861 gcggtcggtg gatatcgaag aaccgtcgga cctcctcccc cttgccggcg caggcgcgct
    19921 ctgcatcatc ctcctgatcg cctacctcgt ccggcgggga gcaccgaagg cgccggagcc
    19981 gccggtcacc cccatccccg ccgacccccg cggcgaggct ctccgcctcc ttgcagcggc
    20041 gcgggatgag tttgaccgcg gcgagcggaa gggagcgtat ggaacggccg gcagggcgct
    20101 gcggtcgtac gtgtcgtccg tatacgggac cggcgcggaa ctcgcggacg gggatgtcct
    20161 gcgcctgctt gaggagagcg ggcgcgagac cggtgcggca tcgtccctcc tcgggcggtg
    20221 cgcggccgtc tcgtatgccg ggtcaccacc cctggacgaa gggttccgcg cggtcgtcgg
    20281 ggagatcgag gcctttatca ggcgagattg atccggcggc gctccgggga aagggttaac
    20341 ccgatatcgt tatccggcac cgggttaaca aaaccaggtt gatacgcgcc ctgcttcagc
    20401 atctttttta tcttcattct cccaggttta tcaggaaaaa ggcgtatcat gaccatactt
    20461 cttgctcttg acgatacgga caaccttgaa tcccggggaa ccggccgcct ggcccgcgcg
    20521 gtcgccgacg cgatcgcgat gcactatccc gtatggggcg tcacccggca ccagctctac
    20581 gtccacccgt cgatacctta cacatcccac aacagctgcg ccgtcatcca catcgtcgcc
    20641 gacggcgcgg aaccgctcga tacgagcctt gccatagccg aggagacgat gcttggcgac
    20701 ttcgtggagg ggagcgaccc gggccttgcc ggagcgcccc tcgaccgggt ggccccggct
    20761 ttgatggcgt ttggccgcga cgcccagcag atggtcctca cccaggagcg tgcccggacg
    20821 ctggcaaaaa acctcggcat atggctgaag ggtcttggcg gcaccgagga cggcgtgatc
    20881 ggcgcgatgg ccggcctcgg gcttgcggct acctacaacg acggcaggtt cctccagaaa
    20941 ggcgccatcc gcagccatac cggcccgaca ccggcaagca ccctcctcgg cgcggggatc
    21001 gacgagatca tgacgcttga cggtcggaag gtcaccgaag ggaccatcgt cctccaggag
    21061 ggcaaagccg ccaaagcctg ccccatcaac ggcagggcgg tcctcttcgt cgacgacagg
    21121 gacggagagc tggtcgcggt aaaaaggaac tgagcatgga gttttggaga agagtcctgc
    21181 tggcggtcgg cgtgctcgtg gtcttcgttg ccgtagcgtt tctggtcgat accaccctgt
    21241 cgcaggccta tgcccgggag agcgcttttg cggactctct cccgcatacc gcggccatcg
    21301 atcgctacga tctccggtac ggcccccagt cctggcggcc ctacgccacg ccgatcgcga
    21361 tcggcccggg cgatctcccc tccggggact ggtacatcgc ctttgtcgag ctgggcgtgg
    21421 tgcccgtcgg tggaaacccg gcccttgccc ggacaggatc ggtccgggtc gactaccggt
    21481 tcacggatct tgccgggacg gcatcgttgg gcgtctacgg tctccacgcc ggggacggca
    21541 gggcatggac gaaccggcag tcgggttacg gcacgtccgg ctactacgtc tccgggaccg
    21601 ccgcgcccgg cgacctgccg cggtggacga ctcccctgga agcgggcaac cgctacgtcg
    21661 tcgaggtggc gggctcgccg cgcccggtag acgaggacgt ggcccgggat acctgcacca
    21721 tctggtttga gcggatcagc tcgggcctgg atgggatcca catcaccacc gatcgtgcgg
    21781 cccccaaagg tcaggtgacc gagaccgccg acctcgacgg ttcgttctac atcacgtata
    21841 ccggcggcaa cagggtcgga gccctcttcc tgatggtcgc cgtggaccgg ccgcagcccg
    21901 aaggtttcgg gcttgcgctc gaatcaacat tcgtggagat tagccgatga tgaaacgtcc
    21961 ctcctacttc agcctgtacg agatcgccct cctctcgctc tttggcgctc tcgtcttcgt
    22021 cctgcggacg gcgatcatag tcccgatcca cgttcccggg agctccggga tattctgggt
    22081 cctgccgctc atcgtcggca ccgccatcgt gagaaaaccc ggggccggca cgtatgccgg
    22141 actggtatcc ggcatcctcg cgtcgttctt cggcatcgag ccgctccacg tcttcgatgt
    22201 attcaagtac actgcgatgg ggatcacgat cgacctcgtc tctatggcgt ttggctaccg
    22261 tctcgagaac cccgtcgtcg gctttgtcgc cggcgccgcg gggaacgtgg tgaagatggt
    22321 ggtcaactac gccgtacacc tgctcctcgg cgtccagggc gtcttcattc tcctcggcat
    22381 cgggttatcg tcggccacgc atatcgtctt cgggggcatc ggaggcatca tcgccgccct
    22441 gatcgtcggg cggctctaca gggcgggtat cgtgcatcag gacgatgtgc cggatgacgg
    22501 ctgacccggc gatcgagttt cggtcggtct cgttccggca cccggcctcc cgggcgaacc
    22561 ggccgcggga ggtgcttgcc gggcttgact gcgtcatcgg ggagggcgag cgcgtgctgc
    22621 tgaccgggcc gagcggctcg gggaagacga cgttcctccg gtgctgcaac ggcctcatcc
    22681 cgcacgcccg cccgggcacc ttcaagggcg acgtctttgt ccggggacga aacacccgcg
    22741 actgcacgat cggcgacctt gccaccaccg tcggcatggt atttcaggac cccgaccacc
    22801 agatcttctc taacgacgtg ataagcgaga tagcgttcgg ccccgaacag cttggctggc
    22861 cgccgtcgcg aattgaccgg gcggtcaacg agatcctgga gcagttccgg cttgaccggc
    22921 tctgcgaccg gcagacgacc gagctctcct ggggagaacg ccagcgtctg gcgatcgcct
    22981 ccgtcgtcgt tctgcgcccg gcaatcctca tactcgacga accgttctcc gggcttgacg
    23041 ccgcgaatgc ggccgggctc cttgacctcc tcgagagact gaaccgggag tccggcatga
    23101 cgatcgtcct ctccgagcac cggaccgaga tggtcgctcg ctgggcgtcc cgccggatat
    23161 cccttctcga cggaaggctg atcgagcccc ccgtcccgga cgctcccctc cgggaccggc
    23221 ctccccgccc gggggcgaag gccgcccgcc cgggggtcgt caccctggag aacgtcgcgt
    23281 tccggcaccc ggggaaagtc tcgccggcga tggacggcgt ctccctcacc ctctacgccg
    23341 gcgaggcgac ggtgctcgcc ggcccgaacg gctcgggcaa gagcacgctg ctgagactct
    23401 taaacggcct cctgcggccg gagtccgggc ggatcgtcgt gaacggaagg gacgtccggg
    23461 agagcaccgt cgccgagatc gcccgcaccg tcggcctggt ctgccagcat gccgattacc
    23521 agctcttcgg ggagaccatc gccgaggagc ttgcttttgc cccggcaaac ctcggcactc
    23581 cccgtgacgt gatagagcag agggtcgaac gtgcgcgcga gggtctcggc atcgagcacc
    23641 tggggcttac cgcctcgccg cttgccctct cggtcgggga gaagcagcgt gtcagcatcg
    23701 catccgtcct gacgatggat acgcaggtcc tcgcgctcga cgaaccgacg ctcgggctcg
    23761 accccgccgc caaacagagg ctggccgggc tcatcgatgc actctgcgcc catggaaaag
    23821 cggtcgtcat cgcgacgcac gatgccgggt tctccgcggc ggtcgcggac aggatcgtca
    23881 ggctcgagcg cggccgcatc gtggacgacc ggagagtgca ggcatgaccg acggggaggg
    23941 gcagcgcgac cggaggcatg cgtaccgggc ggtaggctac ataccggcgg atacgttcct
    24001 tcaccggatg gacccgagga ccaagctcct gaccgtgctt gcgatcagca tcgctgcgtt
    24061 cgccacgata tcgcctctcc gcaccgcaat cctctttgcc ttcgtcgtct gccttgcggt
    24121 tgctgcaggg ctcgtgagac cgttctcccg gggacttctc ctcctcctgc cgctgctggt
    24181 cttcatcgtc gtgatcgaca tattctttcc cgagatctcc tcggggcacg tgtatctttc
    24241 cgcccaggtc ggcccgttct cgtcggtgat aacctccggc gggctgctct atgccgcggc
    24301 aaaggggatc cgggttcttg tcatcggtgg gatatcgttc ctcttcgtga tgaccacatc
    24361 atacacggat ttcgtcgcca gccttcgcat cagccggctg cccccggtca tcgccttctc
    24421 gttagggtat gcgctgaagt cgatgacggc gcttgccgac gatctccgga atatcatgga
    24481 cgcccagcgc tcccgggcgc ttgagttcga ccgggggacg ctcatcaaga acaggaggaa
    24541 actgatggcg ctcggcgtcc cgatgacggt atcgatcctg aaccgggccc gctacgtgac
    24601 cgaagcgatg cagagccggg ggtttgcgtc gaccccgagg cccacctgct accgccctcc
    24661 ccggcccggg aggcacgatc tcctgctgct cgtcgcgctc ggcatcgtct gtcttgcgac
    24721 gctgctgatc cccgtgtagg ggtttccatg acagggggct cgatcagcct cccggctggc
    24781 gcggaagtgg cccgcacggg ccacggcagc agttcaccaa aagatctgca ggggggctac
    24841 tcctgcacta cctttacccg ggtctctctg aagtgccggt cgaaggtata gatctcgctg
    24901 atcccctctc gctccatgac gaccacggca agggcgtcgt tgatgccgat ggcgtggttc
    24961 cgggcgaggt gtatgctctc ccggtagtca tcggcagaga ccgattcgac agtgatggcg
    25021 ggtttcagga gtatggccga gaggagatcc gttgcaaacg ccactcccgc agcatcttcc
    25081 agcacgtttg ccacctcgct cagatgcacg gtggtcgtgg tgaccggttc gccggcattc
    25141 acccggagga agatatcttt tgccgcagcc ttttttcgca ggacggtatc cggcagggcg
    25201 gccttcggtt tcaggatggc gtatatgaag acgtttgcat caatgaaccg cattctttct
    25261 cagttccttc cggaggtcgt ggtaatcgtc gaaatgctcc gcctcgacct caacgctatc
    25321 cacaaaccgc gagagatccg catctgccgg tcgtatctcc aaccggtcgt cttcggcgac
    25381 gatgaccacg tagcctgtct tgaaccggtt gcgccacgat cgcggcagga tgatccgccc
    25441 ctgggcatcg accggcctta tctccacttt tgacataaat caccagggtt acttcgtatc
    25501 accaggtatt aagatttacc ccatcgcccg gatcacaccg gccggcccct cttcacgcct
    25561 ccgccgcctt ctccccgaac tcctttcgga tcccctccgc gaacttctca aacccgatct
    25621 cgtccatctg gtcggcaaga ggccggccgc tccaggcagt ccggtagacc cagtagacga
    25681 tgcggtcgac gacctcgacg acctcctcct cggtctcgac ggtgacgagt tcacgccctg
    25741 cccggggcgt ggtcccccgc cacccgccga ccgtgatctg gtaatggtcg tactccgact
    25801 tcaggagatg gtaggggcag gagtggacgc agacgccgca ctggacgcac ttctcctcgt
    25861 caaggacgga tatgccgttc ttcaacgata tggcgcactg cttgcagtac tccacgcagg
    25921 tgccgcaccc cgtgcagaga ccgggtatgc gcagcggccg gatgcggccg atgatcccga
    25981 tgtcgtttaa gagcggactg ttgcacatgt aggtgcagcc cgatatggcg atgcggaggc
    26041 gtatcgggag ctcctttcca aagacccgcg cgtcgatctt tctggcaagg tcgatcgtct
    26101 cgcagttggc gtacttgcac cgttccgtcc ccgggcaggc catgatgttc accacctcgt
    26161 tctgctctgc cccgatgggt gtcccgttct tctcgagggc ctttgcaacc ttcgcgaggt
    26221 tttcggggtt cacgtgcggg atctccgctg tctggcgcat ggtgaggtgg acggagccat
    26281 ctccgtattt cacgctgatc cgcgcaatct ggcgaagctg gtccgcggtg aggacacctg
    26341 ccggaatccg cagccgtacc gttgcataat cggcgtttcg ctcggtgatg acccctcccc
    26401 gggtgtggag ggtgtagtcc ttcatcatcc tacagtagtg gcggtcgccg ggataaaaag
    26461 aatggttaca ggagggcgag gaggaggatg acccccgccc gggtgatctc gtttgcagcc
    26521 ccgacgacgt cgccgttgac tccgccaaag aggtgccggg agagcgcgag gagcgctgcg
    26581 gcaatcccgg ccgcggcaac gagcgcgagc gcgatcttca tcggagagac cggcagcagg
    26641 aagagaggga gcgcaaggag ggttgccggg acgacgaacc agggccgtgc aaacccgtga
    26701 aggtaggagt ggatcccctc ccggaacggc accccgagcg tggtgaggta ggacatagcg
    26761 accttcgcgg agacctcggc cgtgagaatg gccgcccaga gcggcgccgt atcctggagc
    26821 ccggcaaacg cgacgagcgt gacgatgata cccgccgcga cggcgccggc ccccgtggtc
    26881 cggtcggtca tggccgctat ccgcttctcc cggctcccgt gggccatgag cccgtcgccg
    26941 aagtcgagca ggccgtcgaa gtggttgcac cccgagagga cgagcaccac ggcgagagcg
    27001 acggccgcac ccaccgccgg agatccgatc caggcgacga cgccggccgc tatcccgccg
    27061 atcacgtacc ccgcgagcgg gtagatgtag gagcggcggg cgaagtgctc gaactcgacc
    27121 ggtttcccga gagggagcgc cgtgcagaac tgcaggaggg caaggaccga cttcacatcg
    27181 attcactata gaggcgcgac gtgcagcccg cgatcagccc ggcgacggcg tcgtcgagga
    27241 acggcccgag tttagagagg atccccggct tcttctggtc gtagcgggtg aactcgaacc
    27301 gcgcgtaggt tcccccgatc acctctgcga tggccatccc gatgatctcg tccgagagga
    27361 ggaagaccgg gtcgtcggcg atctcggagt tcttcctcct ccagtagagt tcatcctcga
    27421 gcaggatggc ggaaagaagg agcgacgata cgttggggtc ctcgaaggcc ttcccgatct
    27481 tctcgtccag cttctttgcg gcctcatccg ccccgatccc gtgggagacg tagagatcca
    27541 tcgcggcggc gacgatatcc ctcctcatga tacccttccc ttccagcctg tgctcgatct
    27601 cgaacatgac atagtggttg agcctggcgg tatttgcgcg tttggattcc gtgcgtaccg
    27661 ggttccactg gatatcgcgt cgaaagaccg caacctcttc tacccgcggc acccacctta
    27721 taccggatgt ctcgtccttt tctctcaaaa gaccccggtc tcaggccgcg ccgcccggtg
    27781 atggcgctgg tcctcgcgaa caccatgctc tccaccgtcc ccggcatatc gggtgccggg
    27841 ccgacaccgg aaaagactct cctcacgccg gtcctggacg cggaactggt cacgaccggc
    27901 gcgataacga gcgtgccggc ccggccgaac acccctacgg ggtgcccgac gccggcgacg
    27961 atcacccggt ccatggtgga gctgaccggt ctctcccccg tcttcatcaa cgccgggctc
    28021 gtgcataccc ccaccgtccc ctgcctggac gtctacggga gcgcgggcgg cgacccccgg
    28081 aagggagacg cggtgccgca ggcggcgctc ctcttcgagc ggggagagac ggtcggccgg
    28141 ctccttgcgc agtatagcga cctgttggtg ctcggcgagt gcgtccccgg cggaacgacg
    28201 accgccctct gcgtgctgcg ggcgctcggc tacgacgcct cggtcagcag cgcgttcgta
    28261 gagaaccccc ttgccctcaa agatgccgtc tgcagggata ccctcgcccg gatccgcaaa
    28321 gcgggcgaga gcgagccgct cggtatcctc cgggccgcgg gcgaccccat gatgccggtg
    28381 gcggcaggga tagcaagcgc ctataccggg gatatcctgc tcgccggcgg gacccagatg
    28441 ctcgccgtgg cggctacctt gaaagaactc gggagacggg tgccgcacct tgcgaccacg
    28501 gtctacatca gagacgaccc ctcggccggt ttctcccggt cgatcgccga tatcggcacc
    28561 accgcgtact acgtcgaccc ggacttcgcc ggcctcgggc atgccggcct cgcccgttac
    28621 tgcaccggcg aggtcaagga agggacgggg gccggcgggg cgctcatgct cgcctacctc
    28681 atgggccaca aatccgaggc gatcacccaa aaagtcgttg atttcgtccg gaagtatgtc
    28741 tgagggaagg actatacctt tttacatcca cctataagca atgttttcca cggacgacct
    28801 ttctttgaga gctggctatg ctcggaatag atgatgttgc ggaccgggtc gggtccgtcg
    28861 ggttccggtg ccgggagtgc ggcgcctgct gccgccgggt cgcggaggac tcaaacctcg
    28921 tcatcgtgag cccggccgag gtgcgggcga tcatggcggc gaccggtatg acctggaatg
    28981 aggtcgccgc gccctacccc gagttcatcg acgccgggaa cggcggggag tacaccctcg
    29041 cctggtgcat caggcgcacg aatgaagcct gcatatttct ccgcgacggg cggtgctccg
    29101 tatacgccca ccgcccctgg atctgccgca cctacccgtt catgctcgtg gacgacgacc
    29161 tgcgggtctc ggaatgcccc gggctcggca cgcccctctc tccccgcgaa acccgcacac
    29221 tcggcgccga cctctgccgg cggcaggccg ccgaggcggc ggaggaggcc ggcgtccggg
    29281 ccgtctaccg ggaggcgacg atcccgcccg ggggccgcgc cgtgatcgac agcgagggcg
    29341 tgaaggaggt ccatggctga gatccatctt gtcgggacgg cccacgtctc gcagaagagc
    29401 atcgaggacg tccgttccgc catcgagggg ttcgagcccg atatcgtcgg cgtcgagctc
    29461 gaccggggcc ggtacgtggc gctcaccgaa gagatcgccg agccctcgat cgcggatata
    29521 ctgaagggcg ggaacttcgg ccggctcctc gtccagtggg tgctcaccta catccagcag
    29581 c
//
LOCUS       DAOSEE010000008        29523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_749_length_29523_cov_252.845290, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000008 DAOSEE010000000
VERSION     DAOSEE010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 29523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29523
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_749_length_29523_cov_252.845290"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1356
                     /locus_tag="PLI76_01305"
     CDS             <1..1356
                     /locus_tag="PLI76_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HOI60696.1"
                     /translation="ILEVNETNKMIEQEKLDVRTITLGISLLDCCDADLDALNRNIYD
                     KIAGLARDLVSTGREIELEYGIPIVNKRIAVTPVALVGGRACRSPEDFVEVAKTLDRA
                     ARDTGVNFIGGYSAIVSKGMTPTDETLIRSIPAALAATERVCSSVNVGSTKTGINMDA
                     VKLMGEIVRETAEATKERNSIGCAKLVVLCNAPDDNPFMAGAFHGVSEADAVINVGVS
                     GPGVIKHALEGVRGANFEILCETVKRTAFKVTRAGQLVAQEASERLGIPFGIVDLSLA
                     PTPSVGDSVAGILEEMGLESAGAPGTTAALALLNDQVKKGGIMASSFVGGLSGAFIPV
                     SEDQGMIDAVNRGALTIEKLEAMTCVCSVGLDMIAIPGDTPASTISGIIADEAAIGMI
                     NQKTTAVRLIPVIGKDVGDTVEFGGLLGHAPVQQVNRFGCADFINRGGRIPAPIHSFK
                     N"
     gene            1442..1846
                     /locus_tag="PLI76_01310"
     CDS             1442..1846
                     /locus_tag="PLI76_01310"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOI60697.1"
                     /translation="MVAREDSARFFAADAFARHNGIECVEVAPGRAVVKMEVRGHHLN
                     SHGTVHGGALFTLADTAFALASNSHGVPAAAINAQISYLTAAASGPLYAEAEEYAVNP
                     KLASYTVRITNDAAEKIAIFQGMVYRKTPRRD"
     gene            1854..2375
                     /locus_tag="PLI76_01315"
     CDS             1854..2375
                     /locus_tag="PLI76_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6125 family protein"
                     /protein_id="HOI60698.1"
                     /translation="MITTNSDIFERMTSEELREYLAFLLWHYRVVDAFWFIYIAEEFG
                     QSTAEAVNERVWGRVAPMAAKDLVARFGIREKGLRGFVKALKLFPWAIIVNYDIEERE
                     DEVIITVPSCPSQEARLRRGLGEYVCREMHRAEFTGFARQIDERIVVECLFAPPDPHP
                     EGIFCKWRFYLRE"
     gene            complement(2360..2719)
                     /locus_tag="PLI76_01320"
     CDS             complement(2360..2719)
                     /locus_tag="PLI76_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HOI60699.1"
                     /translation="MIPAAHAVVAPDEAVDREQRGVRMRVRTGEGDPAGTACLEAEPG
                     HAAEFCRETSAFIYPIVEGEGICIPGCPGRGAVVPPKGSVCFRGGTFVRCPSRFPRGR
                     EGHIRDIGCAWSAYSLR"
     gene            2823..3662
                     /locus_tag="PLI76_01325"
     CDS             2823..3662
                     /locus_tag="PLI76_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOI60700.1"
                     /translation="MHRIIDWNELWKALHVSSPERAEKDRNPAAHWDKRAAAYRRVTR
                     DERSATEQELAILGVQPGETVLDMGAGTGRLAVPVARTAAHVTALDPSEGMLAILRER
                     MAAEGLTNYSCLRKRWEDATIGRDVEPHDIVVAAFSLGFYDLAAALEKLDAAARRAVY
                     IFWHAGEWRNPGEMALYRTVFGDEAAGEKGYPDYIFPVNILHDAGIYPNVRIYRAAWD
                     AVYDSVEDAARTWAAMHNPGMEDLTPVREYFARALRKDGSGKYIETAVRPTAAVWWEK
                     EGR"
     gene            3761..5230
                     /locus_tag="PLI76_01330"
     CDS             3761..5230
                     /locus_tag="PLI76_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HOI60701.1"
                     /translation="MQNQPYVDLDAIAWAAMEQYGFIPAFPPSVIREVGALTTNTVSE
                     PAGNPHDLRSLLWSSIDNHDSQDLDQIEYCERGENGAIRVRVAIADVDALVPKRSWTD
                     RHAAHNGTSVYTGVVTFPMLPDSLSAGATSLLPGRDRAAIIIEYTVLPDGSTRPGSIY
                     RALVRNHAKLVYEEVGDWLAGAAPVPETVAETPGLREQILLQHEAAMRMKKRRTEKGA
                     LALETIEAEPVVAGGRVMGLVVQEQNRARCLIEEFMVAANGTLTAFLAAADLPMIHRV
                     VRTPKNWGGIVREAAERGVALPTEPDAEALTQFLIRQKAADPDRFPDLSLTVVKLMGA
                     GEYVAFRPGDIPVGHFALAVTDYTHGTAPNRRYVDLVIQRLAKSVIDGKRYPPYGPDE
                     LDDLALHLTDREKASQKVERFMRKAAAAVLLRDRMGETFEAFVTGASEKGTYVRLIDP
                     PAEGKVVIGEEGLRVGRRVLVRLVAADPYRGFIDFEYTE"
     gene            complement(5311..6192)
                     /locus_tag="PLI76_01335"
     CDS             complement(5311..6192)
                     /locus_tag="PLI76_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOI60702.1"
                     /translation="MEIADILNTTTIPLSEITLERVVLAVIVAVIGWIVVRLIISAFT
                     KALSRASRLPELIVEFLTRFFSILLYVILVLVVLATIGIDVSSMVIGLSAAIGLILGF
                     GLQDTITNLAAGVWVAALRPIDKGEFIEVKGISGKVVAVGIMATELVKPDNTYITIPN
                     SLIWGSPVINSTRMETRRVEVRVRVAYGSDLNLAIRVATDLMKAHEAVLPSPKPVVVV
                     TELADSSVNLALRAWTETPDYWGVQWDLNRDIVLAYREAGVEIPLPQMDVHLDEMRQS
                     HSLSMGDTGTPRSKIRG"
     gene            complement(6410..7102)
                     /locus_tag="PLI76_01340"
     CDS             complement(6410..7102)
                     /locus_tag="PLI76_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HOI60703.1"
                     /translation="MADATQQMVKRTEYSAVNLMKDAGVDAGRWSRLPDDETIRRTVK
                     AIESQNVRVILVDTADEALRAVVDLIPEGAEVMHGTSTTLIEIGYDRVLRENRKGWRD
                     CHAIITSENDAEKRHALRRKSVTADYFLSGVQAITGSGELVGCDKTGSRTGAWPHAAT
                     HLILVSGTNKIVPTLDDALKRCREYALPLEEQRARHVYGTGSSIGKYVILEREDTDGR
                     VTLVLVREQLGY"
     gene            complement(7197..7778)
                     /locus_tag="PLI76_01345"
     CDS             complement(7197..7778)
                     /locus_tag="PLI76_01345"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOI60704.1"
                     /translation="MDPYPEGQAAMMPEVIDIATIFGPVHLIWTRSQSGPKVRRIVLP
                     HDLPTSVAAFEDAPPDGELPDDSIRALIAGIRSFLQGNDVRFDIDLLDLDRCPLFQRR
                     VLLAEFGIPRGYVSTYGRIARHLDTPGAARAVGNALARNPFPLVIPCHRVLRSDGRIG
                     GFQAGLAMKRRLLEMEGVGFREDGRAIMERIWY"
     gene            7911..8867
                     /locus_tag="PLI76_01350"
     CDS             7911..8867
                     /locus_tag="PLI76_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOI60705.1"
                     /translation="MKEWHLIGMACLLILAAGCSGQGGQGNAGENQIHADPAGFVREA
                     AAFAESAGKDAALAEFGNASGRFSRGSLYIYACDYNGTLLAHPYASEAVGANRGNWTD
                     VRGLPAIRIGAATAANGGGYIAYLYPTPENGSIDEAAEETSLPKIVYVAPAGETWWVA
                     SGSYLSGAAGPGTEPYPEPVSAMIDLVERGAAYGRDHGRATAFRAISNQTGTFVDAAG
                     HYLYAYDYNGTLLAHPYLQAAVGSNLIERQDPFGMKNIRALRDTARDGGGFVVFVWPN
                     PDRENREELKIGYVLPVDETWWVGSGVYLSEITGVDASLPSP"
     gene            complement(9036..9347)
                     /locus_tag="PLI76_01355"
     CDS             complement(9036..9347)
                     /locus_tag="PLI76_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60706.1"
                     /translation="MKGGTEERKSGEPGPVTFTCYRVAPEEENAPGEGMNTYSVVVLL
                     PGGDVRHQVVWAGAPEDIGDAIRVPPGSRVIMTEMASALVWDSENVEEEEAAVEAAPT
                     P"
     gene            complement(9980..11485)
                     /locus_tag="PLI76_01360"
     CDS             complement(9980..11485)
                     /locus_tag="PLI76_01360"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HOI60707.1"
                     /translation="MRDSLILMNPSDLVCFLKKDPAEFTREDIIRFCEANRIEMVNFR
                     YAAEDGKLKTLNFVISSKEHLDTILSDGERVDGSNLFSFIEAGSSDLYVIPRYRTAFV
                     NPFAEVPTLELLCSFYDSDGKPLESSPEYVLRKADEEFTRRTGCTFKTLGELEYYVVS
                     PRGDLYPGRDQKGYHSADPFVKFADLRDEAMRLIARAGGMIKYGHSEVGCFFAGDTYY
                     EQHEIEFLPMPVEQAVEQVIIAKWILRMLGNQYGVEISFAPKITVGKAGSGMHFHMLA
                     EKDGQNLMVEGGRLSPLARKMIAGILDVSDALTAFGNTIPTSYLRLVPHQEAPTRICW
                     GDRNRSVVVRVPLGWIGAEGMTHDANPCENRHRDERSSKQTFEYRVADGSADPYLTVA
                     GLVVASLHGIGMPGALEAAEKLYVSGNIFRPEFKERLAELEHLPASCVESAGALAAKR
                     AIFEKDGIFPAGMIDSRIAALKAFGDRGLNERIYGDNEAIRELVEQYIHVA"
     gene            complement(11772..12755)
                     /locus_tag="PLI76_01365"
     CDS             complement(11772..12755)
                     /locus_tag="PLI76_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HOI60708.1"
                     /translation="MCDKDPDRHARPRMVLPCALLLTCLALFLTIVPVGLTGAPPARE
                     QPDPAIVKIIAEVNESELWNTTYDLQAIPTRVFGSEGNREAGEYLYARLDGIPGLDVE
                     FQGGELNNVVATLPGSGNASSELVIVGAHYDSTSSDPARAPGATDNACGVAIVMELAR
                     VMSGHSFDRTVQFAFWNAEEVGSYGSIDYATHAMDTGVPVVLYLNYDSACYDPENRFI
                     LDIMYDSGSADIAALMADHNTLYGTNFTLTRNRHACESDHTSFQALGFPALMTHSEEH
                     GPAHTPEDTIDHVSSGYMAENARLGLSVLAKVAGPRDAGEGAAIPKAPTAG"
     gene            13011..13442
                     /locus_tag="PLI76_01370"
     CDS             13011..13442
                     /locus_tag="PLI76_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF61 family protein"
                     /protein_id="HOI60709.1"
                     /translation="MPDRPSAGDESTLLRWMRLEIGRINDGIVSERKRLSRLLSEERP
                     SSVTKGGSEYGFDRDILHRLEQALPGELQRRLRLPILFYFDSTVPDSLFLADEAAMQA
                     LQSLGEISPLRRMQGGRLWIAKPLVYAIMNRYPTVMQVVMG"
     gene            complement(13526..14077)
                     /locus_tag="PLI76_01375"
     CDS             complement(13526..14077)
                     /locus_tag="PLI76_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60710.1"
                     /translation="MSEKELKIGDIAHLTGISEQDVRTLVRTYESLFSYRTIGPVRLY
                     PEKAVAIIREILELSGKGLTPGEIAEEVGTGRRSGAQEEPAEEVGRTGAALPPGLVFD
                     LPVIQDTLARQERWIARLTDELEREREEVARLQDAVDHLQADLDRQQGQLAVVAEWVD
                     YFDRRMDEVARPVLERFRQTFGR"
     gene            14203..14886
                     /locus_tag="PLI76_01380"
     CDS             14203..14886
                     /locus_tag="PLI76_01380"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOI60711.1"
                     /translation="MNRGREGDVPEGTTVIRKAVRSVRLQVRPDGTLRVVAPPSFDVG
                     GFLQRNAAWIDERREEFDRMAAEGRGKEDQLLLHGRFYRPVPGARFSIDETRETVAFP
                     SVSALRRDLSGMLKEETLVRLKTYPDLTGPWSGRIAVKLQTTRWGSCSSLGNLNINLR
                     AVALPDTLREYIVVHEAAHLRERNHSKRFWLLVGDRYPGYRAAESELRRYWVILERNR
                     IWGSLQDVG"
     gene            complement(15145..16698)
                     /locus_tag="PLI76_01385"
     CDS             complement(15145..16698)
                     /locus_tag="PLI76_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOI60712.1"
                     /translation="MGDQDSRGGEAFIAAGTRDVAAAVLQQVIETSLSGTCIIDREGC
                     IVSASAPLLAIWGYSRENVVGKPVDILWPSRGERPDWIGQALSTGRWAGTVVARRGDG
                     SLFDVRISAGTARDPGTGHPWLILSCLDAAAQQRPEETPGQRRSLECAVAAMPARFID
                     SSQIDPAIDDSLRDLGRACGACRAYLALFNDAKAILGTTHEWCAPGQRPRRGEFQKIH
                     SGNLPRWIGRILNGEMVYMDGTPREGQTQEEERDLLKRHCTTAALMIPLRLHGKVVGF
                     VGLDRCIGDTRLANEDIALIHAAVHIIASALDRKQSEYLFRESENLYQIVLDAITDAV
                     IVVDTGLTLLLANDAFIAWCEELGLATDVVGKDIFTVLPFFPPSTRQQYERVIRTGRP
                     LTSERSFRIDDRTVTLREIRIPIIERGEVARIVTVARDITAQREVEDLRTKAYAQIER
                     NMEQFALLADHIRNPLQAIIGRAELLDDAGATEKIRQQVQRINAIIDQLDERWAESRK
                     LSMFWRKYS"
     gene            complement(16985..17665)
                     /locus_tag="PLI76_01390"
     CDS             complement(16985..17665)
                     /locus_tag="PLI76_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HOI60713.1"
                     /translation="MHQTGTWGDDGVESRANLLTVSVPLLLCGIFFAVVGLFLPAEQW
                     FLLAGMMVAYIVPPLGRETIIPVSILLGIPWWLIAFTLAFLDFAGGLFMAWNFPLVLR
                     VPYVGPRIRRFMTAGRAYLDRRPWLERLYFVGLIAFVSLPFDGSGSIIGSVLGRMLGM
                     TKIEVLSCITIGGIIGSFTIALGIDYIVNLIPAGAGTWVSAAVFLLIGIALLVMYRSY
                     SRGWGADA"
     gene            complement(17689..18132)
                     /locus_tag="PLI76_01395"
     CDS             complement(17689..18132)
                     /locus_tag="PLI76_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthase"
                     /protein_id="HOI60714.1"
                     /translation="MIRVVATGTFDILHPGHLYYLEESRNLGDELSVIVARDANVKHK
                     PRPILPEDQRLQMVRALKPVDRALLGDIHDMFRPIAELKPDIITLGFNQHFDEENLRR
                     RLRERGLDADVVRISGYPGLLASSSKIVEHILKTRGPTPPTGEEE"
     gene            complement(18129..18500)
                     /locus_tag="PLI76_01400"
     CDS             complement(18129..18500)
                     /locus_tag="PLI76_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome protein"
                     /protein_id="HOI60715.1"
                     /translation="MAIRGISRDLLMMLFELGKEHHPNEFVAVLRERSGIIQDLDLVP
                     GTIVGEESASFFIDMLPLDIHQAGSAHSHPNGVLSPSPADLRFFPTVGRYHIIVGYPY
                     GERDWRGYRADGHPARLEVVG"
     assembly_gap    18680..18779
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18780..19647)
                     /locus_tag="PLI76_01405"
     CDS             complement(<18780..19647)
                     /locus_tag="PLI76_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOI60716.1"
                     /translation="MRRDPIIFGFITALVIGLVALPALAATAGVDIQGGSYHPASLTV
                     ESNTTVTWTNHDRIGHTVTSTDGLFDSGPIEHNETFNYTFTDTGTYPYGCTLNASTRR
                     TPMQGVVIVVPEGMAVAPDGNRTSGEEPGNATLADLVSGSDLLTTFGEAVGKAGIAQT
                     VLAASGPYTVFAPDDAAFAALGNETVAALFNDTETLDTLLMHHVVRETYTVNDLTAKA
                     NAAGNRTVLTTLTGDNLTVSTTDGNLTVGNATIVASDMAASNGVVQIVDTVLVPPGLV
                     LPENQTAVGNQTA"
     gene            complement(19866..21290)
                     /locus_tag="PLI76_01410"
     CDS             complement(19866..21290)
                     /locus_tag="PLI76_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase-like protein"
                     /protein_id="HOI60717.1"
                     /translation="MHILLPTGSATAAIVKAAAERFRSRYDIDVAVTGDIASFLAPGD
                     LIELLSGRDYDMAIVSGMCTASFAGVERETGVPVYRGPRHAADLALILGVLDRIRLSK
                     TIPADDFLTETRRKEACRRVALREAEAGADFVIRGVKIGGGSRMKVLAEIMDAHRRGD
                     IADEVRRFFAEGADIVDLGFGFDAAPGDVERCFAALEDLDGPLAVDTQDPLLIAAALP
                     RADLVLSLHEGNIPVVGAAIADAGAAAVVVPRERTLRENLAAAGEAGISRLIADPLLQ
                     PVGSGLVDTLSGFPAIPYPLFFGAGNVVELLDADSTGANALLAGMAHEVGAAVIFTSE
                     HSDKTRGSVAEMRRATDMMALMADRPYPKDLGLDLLILKEKRRRREPPLEYGSIVDAC
                     PAPDEIVYDPLGCIRIGIEEDCIVAVHKGRAVRGKHWEDVFYTLLANGSLSRLDHAAY
                     LGKELFKAELAIRLRRSFEQDGPF"
     gene            21334..21999
                     /locus_tag="PLI76_01415"
     CDS             21334..21999
                     /locus_tag="PLI76_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOI60718.1"
                     /translation="MLRCDLHIHTRFSRDGESSVEEILERAEMVGLDAIAITDHDTVE
                     GALYALRCDTPLTVIPGIEVSTRQGHLLALGVTRPLPAGRDFFETVALARARGALLIL
                     PHPYHRWRHGVGRRLAAGIEAVDAVEVFNSRYITGSANRKAAVIARRFGKPGVAGSDA
                     HNARYVGFGVTYITAKPDAASILSAIREGRTMAGGRMTPLHTYTHQSLRGAFRKIRRA
                     MQR"
     gene            21996..22799
                     /gene="truA"
                     /locus_tag="PLI76_01420"
     CDS             21996..22799
                     /gene="truA"
                     /locus_tag="PLI76_01420"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOI60719.1"
                     /translation="MNLVLRFSYFGDRFFGSQVQPLHRTVEGEFIAAARRLRLFDDPR
                     AAHFATAGRTDRGVHARGQVCSFATDIPERAIARLNRVLPEDIWCTAWTVAPEGFHPR
                     YSAVSRTYRYYFFPAPEEIAAMHEAVQEFVGLHDFSAFSRAGDKNPLRRILAARVFEE
                     GPFAVFEVTGESFLWNMVRCMATALKRVGNGKIDRGAIALLLACPAGEGLPAAPPEGL
                     ILWDVEYGFPFKPLPADERSRRYPVGRHRYHMLMAELSARLVPGEGPPG"
     gene            23265..24422
                     /locus_tag="PLI76_01425"
     CDS             23265..24422
                     /locus_tag="PLI76_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOI60720.1"
                     /translation="MYDKDLVRARPPAHHEGWFAEETAVPLPQFMDTETDRWIERHVP
                     QKIGEGSPMDIPALRCEVQEQVNPLRMPTGPGHRRVAGEVAGPAAAPSRRSARVRTLA
                     AIPCFNEEIAIGSVVLMARLHADEVLVIDDGCTDGTATVARDAGATVISHGAQMGKGR
                     GIRSALRYAVDHDYGCLVFMDGDGQHNPGEIPLLVEPILTDTADLVIGFRTFDQMPFY
                     RRFGRAVLDVASSNGSSITDSQCGFRALNRTAIDAMLDGLKKDDFSTESEMLRTAREN
                     CLRVGEAPINCKYGGDLSTSTKNPISHAIEVLGSIFWLAVERKPLLHIGLPGLAAVLA
                     GIFLLLQFFRGFGETGLVAIEQGMLASLFLIPGTVALIVGLMLALIARLRE"
     gene            24460..25539
                     /gene="wecB"
                     /locus_tag="PLI76_01430"
     CDS             24460..25539
                     /gene="wecB"
                     /locus_tag="PLI76_01430"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOI60721.1"
                     /translation="MIGIILGTRPEIIKMSPVIRECERRGLDYFILHTGQHYSYEMDR
                     LFFEELELPDPDYNLDVGSGSQAGQTAKIMTGVEDILVKEAPDLILVQGDTNTVMAGA
                     LAASKLHVRVGHIEAGLRSFNRRMPEEINRILTDHMSDYLFAPTENARKNLLAEGIAD
                     RRICVTGNTVVDAVYQNLDISRKKVSVLKDLDLKSREYFLVTAHRQENVDNRARLKEI
                     LRGLLGVQKEFALPVVFPVHPRTEKRIKELGIGIDGLNFTKPFGFLEFLQLESQAKAV
                     LTDSGGVQEETCVLGVPCATMRYDTERPETLNVGSNVLVGADSRKILEGVRFAVTRKP
                     GWENPYGDGIAGKMIVMVCVAAQSQ"
     gene            25560..26933
                     /locus_tag="PLI76_01435"
     CDS             25560..26933
                     /locus_tag="PLI76_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOI60722.1"
                     /translation="MKICVLGLGHIGLPTALLFAAHGADVVGVDVKQSVVDCLNHGSL
                     PFREPGLEDLYREAEERFAARARPGTADVFLIAVPTPLDQATKVSDLSYVKKAADSIA
                     PHLARGNLVILESTVPPGTSERVVIPRLEKKGIPIGDFLYAHCPERAIPGRTLQEMTE
                     NSRIIGGYDRDSTDRATSIYQTFVRGQIYQTDTRTAEFVKLMENTCRDVNIALANEFA
                     QLAEECGINVWEAITLANKHPRVSILNPGPGVGGHCIAIDPWFLTENSTRCRMVSTAR
                     EVNDSMPNYVLHVVRNLLAGTRDPTISIFGVAYKGNIGDTRESPAFKFIQLAENEGYA
                     IRCHDPYVGEFPHPLVNLADAAAGSDCIVVLADHDCFRTIDPAGLRVRTKLVIDARNI
                     LDHEKWTDQGFAVRVLGDGSSVQQLPPGEVMPPAALYSTDAIRPIAPSLPPSISLLSP
                     NGRGDFL"
     gene            27008..28216
                     /locus_tag="PLI76_01440"
     CDS             27008..28216
                     /locus_tag="PLI76_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOI60723.1"
                     /translation="MRRVCIVSQHFPPEKSGNASRIYDTAVHLADLGLDVTVLAPHPT
                     FPTGSFPRTWRRSSVQEVNGVHVVRLWTWQPASGDPGFPSRMAYYLLFPLHAALWLIF
                     AQNRFDVIVTSAPPLFTGLPGYILRRTSAVKWILDIRDLWIDASIGLGFLKEGSISER
                     LSRRFEQACLSRADFVGVTTEELGRRIASRYRVAAPMELMPNGVDTDLFHPTSGGKKR
                     QIIYAGNVGHAQDLDKVALAIKSMNGTYDLKFLIVGDGDTKKHLEQLVKTENLADSII
                     FTGTLPREEIPRLLSESLVGMAPLKRLANLEYAAPTKAYEYMACGLPFVGCGDGEIAH
                     LAHESGAGVIAGNTPEAIAATLATLLDDPGRMEEMGRRGREYVAEHYGRRAVALKLKQ
                     HIERMTWTSA"
     gene            28201..29388
                     /locus_tag="PLI76_01445"
     CDS             28201..29388
                     /locus_tag="PLI76_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60724.1"
                     /translation="MDERLTLLLSRVAGKVRELWVTDGETAYIRDPVFPVIRNRVHAE
                     FCKAMLRSGGNTLVGPILNYVAACQNPDGSWNEVHVNYNEPSALITAFIGDALIEAAD
                     RHPHEDALERARDFVLAAERSPGCFLKSAGYTADHLNVDASCGAFLARYAERYDDREA
                     RAAARRAAENVVSHQRDGVYPYAVDKGTYPYVFDLPCIHYQGVTIYYLAKTNDVLCDD
                     RIDRSIAEAARWLAAAQRPDGRFDWSGSGLSFACYLSGAYAFAQASFTYASRLDDRYR
                     EHADRSLGWLDANVRGLAPRWEPGSWGDLPLSVAAAVKSASLGGYPLRHRAFRFGYGV
                     YREVARRRYSATAETRAFEALSGLLGIRSSTVEPSKNFPDLFMTSEMLDCLSQSMIWG
                     KNT"
BASE COUNT         5445 a         9257 c         9333 g         5388 t
ORIGIN      
        1 attctcgagg tgaacgagac caacaagatg atcgagcagg agaagctcga tgtccggacg
       61 atcactctcg gcatcagcct cctcgactgc tgtgatgccg atctcgatgc cctgaaccgg
      121 aacatatacg ataagatcgc agggctcgca agagacctcg tctcgacggg aagagagatc
      181 gaactcgagt acggcatacc gatcgtcaac aagcgaattg ccgtaacccc cgtcgcgctc
      241 gtcggcgggc gggcctgcag gtcgccggag gactttgtcg aggtcgcaaa gacgctcgac
      301 cgggccgcgc gggataccgg ggtcaacttc atcgggggat actcggcgat cgtctcgaaa
      361 gggatgactc cgaccgacga aaccctcatc cgctcgatcc cggcggctct cgccgcgacc
      421 gagcgggtct gcagctcggt gaacgtcggc tcgacgaaga ccgggatcaa catggatgcc
      481 gtcaagctga tgggggagat cgtgcgggag acggccgagg cgacgaagga gagaaactcc
      541 atcggctgcg cgaaactggt cgtcctctgc aacgcacccg acgacaaccc gttcatggcc
      601 ggagcgtttc acggtgtttc agaggccgac gcggtcatca acgtgggcgt aagcggcccg
      661 ggggtcatca agcacgcgct cgagggcgtt cggggagcga acttcgagat cctctgcgag
      721 acggtcaaga ggacggcctt caaggtcacc cgcgcagggc agctcgtcgc ccaggaggcg
      781 tcggagagac tcgggatccc gttcgggatc gtggatctct ctcttgcccc caccccctcc
      841 gtcggggaca gcgtcgccgg gatcctcgag gagatggggc ttgagtcggc cggtgcgccg
      901 gggacgacgg ccgctctcgc tctcttgaac gaccaggtga agaagggcgg gatcatggcg
      961 agctcgttcg tcggcgggct ctcgggggcg ttcatcccgg tcagcgagga ccaggggatg
     1021 atcgatgcgg tgaaccgcgg cgccctcacg atcgagaagc tcgaggcgat gacctgtgtc
     1081 tgctcggtcg gcctcgacat gatcgcgata ccgggcgaca cacccgcttc gacgatatcc
     1141 ggcatcatcg cggatgaagc cgccatcggt atgatcaacc agaagacgac cgccgtccgg
     1201 ctgatcccgg tgatcgggaa ggatgtcggc gataccgtcg agttcggcgg gttgctcggg
     1261 catgcgccgg tccagcaggt gaaccggttc ggttgcgcgg acttcatcaa ccgcggcggg
     1321 cggatccccg caccgattca cagtttcaag aactgagccg cccgggaaga cccggctcct
     1381 ttttcgcgac tctcgcaccg gactgatagg cttattacgg tcaccggaga gattacccca
     1441 tatggtcgca cgagaagact cagcccggtt ctttgccgcc gatgcctttg cccgacacaa
     1501 cgggatcgag tgcgtcgagg tcgcgcccgg cagggcggtg gtgaagatgg aggtccgggg
     1561 tcatcactta aacagccacg gaaccgtcca cggcggagcg ctcttcaccc tcgccgatac
     1621 cgcctttgct ctcgcatcga actcccacgg ggtcccggcg gcagcgataa acgcccagat
     1681 atcctacctc acggccgcag cgtccggccc tctctatgcc gaggcggagg agtacgccgt
     1741 gaatccgaaa ctggcctcct acaccgtcag gataacgaac gatgccgcgg agaagatcgc
     1801 catcttccag gggatggtct accgcaagac accccggcgt gactgatagg gtggtgatta
     1861 cgacgaacag cgatatcttc gagaggatga cctcagaaga actccgggag tacctcgcgt
     1921 tcctcctctg gcactaccgg gtggtggatg cgttctggtt catctacatc gccgaggagt
     1981 tcggccagtc taccgcggag gcggtcaacg agcgtgtctg gggccgggtc gccccgatgg
     2041 ctgcaaaaga cctcgttgcc aggttcggta tccgggagaa ggggcttcgg ggtttcgtga
     2101 aggcgctgaa actcttcccc tgggctatca tcgtgaacta cgatatcgag gagcgggaag
     2161 acgaggtcat catcaccgtc ccctcgtgcc cgagccagga agcacggttg cggcgcggcc
     2221 tcggtgaata cgtctgccgc gagatgcacc gggcggagtt cacggggttt gcacggcaga
     2281 tcgatgagcg gattgtcgtc gagtgccttt ttgctccgcc ggacccgcac ccggagggca
     2341 tcttctgcaa gtggcggttc tacctgaggg aataggcgct ccacgcacaa ccgatatccc
     2401 ggatatgtcc ttctcttccg cgggggaacc gtgacgggca ccgcacaaaa gtcccccccc
     2461 gaaaacagac gctgcctttc gggggcacga cggcacccct gcccggacag ccggggatgc
     2521 agatcccctc cccctcgacg atggggtaga tgaacgcgct cgtctcgcgg cagaactccg
     2581 ccgcgtgccc cggctccgcc tccaggcagg cggtcccggc cgggtcgccc tctcccgtac
     2641 ggacccgcat ccggacaccg cgctgttcac gatcgaccgc ctcatcgggc gcgacgaccg
     2701 cgtgagcggc agggatcact cgagggagtc acgggcagcg cggcctgccg tgagtcgttc
     2761 tgccggagat acgatccggt gaagaacaga caagatatac gattacacgg aaaggaacat
     2821 ccatgcacag gatcatcgac tggaacgagc tctggaaagc gctccacgtg agttcgcctg
     2881 aacgtgccga gaaagaccgc aatcccgccg ctcactggga caaacgcgcc gccgcctacc
     2941 gccgggtcac ccgcgatgag cgatcggcaa ccgagcagga actcgcgatc cttggggtac
     3001 aacccggcga gaccgtgctc gacatgggtg ccgggacggg gaggctcgcc gtgccggttg
     3061 cccggaccgc cgcccacgtc acggcccttg acccttccga gggcatgctc gccatcctcc
     3121 gggagcggat ggccgcagag gggctcacga actactcctg tctccggaaa cgctgggagg
     3181 atgcgacgat cgggagagac gtcgagcccc acgacatcgt cgtcgcggcg ttctcgctcg
     3241 ggttctacga tcttgccgcc gccctcgaga aactcgacgc cgcggcccgc cgggcggtct
     3301 acatcttctg gcatgcgggc gagtggcgga accccgggga gatggcgctt taccgtacgg
     3361 tcttcgggga tgaagcggcc ggagagaagg gctacccgga ctacatcttc ccggtcaaca
     3421 tcctccacga tgccggaatc tacccgaacg tccggatcta ccgtgccgcc tgggatgccg
     3481 tctacgattc ggtcgaggat gccgcccgga cctgggcggc gatgcacaac cccggcatgg
     3541 aagatctcac ccccgtcagg gagtatttcg cccgggcgct ccgcaaagac ggatcgggaa
     3601 agtacatcga gacggcggtg cggccgaccg cggcggtctg gtgggagaaa gaggggcggt
     3661 gaggggccat cccctcatcg gccggcattc atcctcccgc aacgggcgta gacgatctat
     3721 tccgggcagt ttttatagcc gcaaacgaat ttctccagca atgcagaacc agccatacgt
     3781 cgacctcgat gccattgcgt gggcggcaat ggagcagtac ggcttcatcc cggcgttccc
     3841 gccgtccgtt atccgggaag tcggcgctct cactacgaat accgtttcgg agcctgcggg
     3901 caatcctcac gaccttcgct cgctcctctg gtcgtcgatc gacaaccacg actcgcagga
     3961 tctcgaccag atcgagtact gcgaacgggg ggagaacgga gcgatccgtg tccgggtcgc
     4021 catcgccgac gtcgatgcgc tcgtcccgaa gagatcgtgg acggaccgcc atgccgccca
     4081 caacgggaca tcggtctata ccggcgtcgt gacgtttccc atgctccccg actctctctc
     4141 ggctggcgcg acctcgctcc tgcccggcag ggaccgggcc gcaatcatca tcgagtacac
     4201 cgtcctcccg gacgggagca cgaggcccgg cagtatctac cgggcgctcg tcaggaacca
     4261 cgcgaaactc gtctacgagg aggtcggcga ctggctcgcg ggggcggccc ccgtccccga
     4321 aacggtcgcg gagacgccgg gtcttcgcga gcagatcctc ctccagcacg aggccgcgat
     4381 gcggatgaag aagcgccgga ccgagaaggg agcgctcgcc ctcgagacga tcgaggctga
     4441 accggtcgtc gccggcggtc gggtcatggg gctcgtcgtc caggagcaga accgggcgcg
     4501 ctgcctcatc gaggagttca tggtcgcggc aaatgggacc ctgacggcgt tccttgccgc
     4561 cgccgatctc cctatgatcc accgggtcgt ccgcaccccg aagaactggg gcgggatcgt
     4621 ccgggaggca gcagagcgcg gcgtggccct gcctaccgaa ccggacgccg aagcgctcac
     4681 gcagttcctt atccggcaga aggcggccga ccccgaccgt ttccccgacc tctccctcac
     4741 ggtggtgaaa ctgatggggg ccggggagta cgtggcgttc cggccggggg atatcccggt
     4801 cggccacttc gcccttgccg tcaccgacta cacccacggc accgccccga accggcgtta
     4861 cgtggacctg gtcatccagc gcctggcgaa atcggtcatc gacgggaagc gctatccccc
     4921 ctacggcccc gacgagctcg acgatctcgc cctccatctc accgaccgcg agaaggcctc
     4981 gcagaaggtc gagcggttca tgcggaaggc ggcggctgcg gtccttctcc gggaccggat
     5041 gggcgagacg ttcgaggcct tcgtcaccgg cgcatcggag aaagggacgt atgtccggtt
     5101 gatcgacccg ccggccgagg gaaaggtggt tatcggcgag gaagggctca gggtcggccg
     5161 gagggtgctg gtccgcctgg tggcggcgga tccctaccgg gggttcatcg acttcgagta
     5221 taccgaatga agggaatcct ttccgccggc agcatggccc gggccggagt tgattcacga
     5281 gggatgtgaa cgagagggaa ggttccgggc tcacccccgg atcttcgatc ggggagttcc
     5341 cgtatctccc atggataagg aatgagactg cctcatctca tccaggtgga cgtccatctg
     5401 cgggagaggt atctcgaccc cggcctcgcg gtatgctaaa acgatgtcgc ggttcaggtc
     5461 ccactgcacg ccccagtagt ccggcgtttc cgtccacgcc cggagggcga ggtttactga
     5521 agaatcggcc agctcggtga cgacgaccac gggttttggc gatgggagca ccgcttcgtg
     5581 ggctttcatc aggtcggtcg cgacccggat ggcgaggttg aggtcgctcc cataggcaac
     5641 cctgaccctg acctcgacac gccgtgtctc catgcgggtg gagttgatca ccgggcttcc
     5701 ccagatgagg gagttcggga tggtgatata cgtgttatcg ggtttcacga gttcggtcgc
     5761 catgataccg accgcgacga ctttcccgga gatgcccttg acctcgatga actcaccctt
     5821 atcgatgggg cggagagccg ccacccagac cccggcggca aggttggtga tggtgtcctg
     5881 gagaccgaag ccgaggatga gcccgatcgc cgccgaaagc ccgatcacca tcgaggagac
     5941 gtcgatcccg attgttgcga ggactacgag gacgaggatc acgtagagca ggatcgagaa
     6001 gaaacgggta agaaactcaa cgatcagctc gggcagacgc gatgcccggg agagagcctt
     6061 tgtgaaagca gagatgatca accgcacgac gatccacccg atcacggcga cgatcacggc
     6121 gagaacgact ctctcaagcg taatttcaga gagcgggatg gtggtggtgt taaggatatc
     6181 tgcaatctcc atatactcaa gcgtggcgta tcggaacaga taatactata cggcctgacg
     6241 gggcgaggaa gaggacgttg tactatcgtt gcgtcggcac tcccgccgcg gggccggttc
     6301 cgggaaggca tgatatccgg gcagggagcg gagccgttgc aaaaggggga tgtgataggg
     6361 atgcggctgc cccgaagcgt gcccatccag cgggcgaata caccccggtt cagtagccga
     6421 gctgctcccg gacgaggacc agggttaccc tcccgtcggt gtcctcccgc tcgaggatca
     6481 cgtacttgcc gatagaactc ccggtgccgt agacgtgccg ggcccgctgc tcttcgagcg
     6541 gcagggcgta ctcccggcag cgcttgagcg catcgtcaag ggtcggcacg atcttgtttg
     6601 ttcccgagac gaggatgagg tgggtcgcgg cgtggggcca cgctcccgtc ctgctcccgg
     6661 tcttgtcgca cccgacgagc tcccccgacc cggtgatcgc ctggaccccg gagaggaagt
     6721 agtcggcggt cacgctcttt ctccggagag cgtgccgctt ctccgcatcg ttctcggagg
     6781 tgatgatcgc gtggcaatcc cgccacccct tccggttctc cctgagcacc cggtcatacc
     6841 ctatctcgat gagcgtcgtc gaggtgccgt gcatgacctc ggccccttcg gggataaggt
     6901 cgacgacggc ccggagcgcc tcatcggccg tatccacgag gatcaccctg acgttttggc
     6961 tctcgatcgc cttcaccgtc ctccggatcg tttcgtcgtc gggtagccgg ctccaccgcc
     7021 ctgcatccac cccggcatcc ttcatcaggt tgactgcact gtattccgtc cttttcacca
     7081 tctgctgggt tgcatctgcc atccgatcac cccagggtgg gaggatgcac tcccacatat
     7141 acctctccat ggatcaggga gggtggtggc gaggggtgtt tactcccgca gagggttcaa
     7201 taccagattc gttccatgat cgcccggccg tcctcccgaa acccgacccc ctccatctcg
     7261 agcagccttc gtttcatggc gagtcccgcc tggaatccgc ctattcgccc atccgatcgc
     7321 aggacgcggt ggcaggggat gacgaggggg aaggggtttc tcgcgagcgc gttgccgacc
     7381 gcacgcgcgg ctccgggggt atcgaggtga cgggcgatgc ggccgtatgt gctgacgtat
     7441 ccccggggga tgccgaactc ggcaaggagc acgcgccgct ggaagagggg gcaccggtca
     7501 aggtcgagga gatcgatatc gaaccgcacg tcgtttccct ggaggaagga tcgtattccg
     7561 gcgatcagcg cccggatact gtcgtccggg agttccccat ccgggggcgc atcctcaaac
     7621 gccgccacgc tggtcggcag atcgtggggg aggacgatcc tccgcacctt cggtcccgat
     7681 tgagatcggg tccagatgag gtgaaccgga ccgaagatcg tcgcgatatc gatgacttcc
     7741 ggcatcatgg cggcctggcc ctcagggtat ggatccatga tccggatagg cggtcgcccc
     7801 tatctaactt atggtcaggc ccctgtgggg atcaggtgca gggggatccc tattttccct
     7861 atccgggaga tttcgttacg cttttctggt atatcgccct acgcacggat atgaaggaat
     7921 ggcacctcat aggcatggcc tgcctcctga tcctggcggc agggtgcagc ggtcagggcg
     7981 gacaggggaa cgcaggagag aaccagatcc acgcggaccc ggcaggtttt gtccgggagg
     8041 ccgccgcctt cgcggagagt gccggaaagg atgcggccct cgccgagttc ggcaacgctt
     8101 ccggccggtt ctcccgggga tccctgtaca tctacgcctg cgattacaac ggaacgcttc
     8161 ttgcccatcc ctacgcgagc gaggcggtcg gggcgaaccg cgggaactgg accgacgtgc
     8221 ggggattgcc ggctatccgg ataggtgcag caacggcggc aaacggcggc gggtacatcg
     8281 cctaccttta cccgacacct gagaacggtt cgatcgatga ggcggcagaa gagacctctc
     8341 tcccgaagat cgtctacgtc gcccctgccg gcgagacctg gtgggtggcc tcaggcagtt
     8401 acctctccgg tgcggccggg cctggaaccg aaccgtatcc ggaacccgtc tctgcgatga
     8461 tcgaccttgt cgagcgcggt gccgcctatg gccgcgatca cggcagagcg accgcattca
     8521 gagcgatctc gaaccagacc gggacttttg tcgatgccgc ggggcactac ctctacgcct
     8581 acgactacaa cgggacgctt ctcgcccatc cctaccttca ggccgctgtc gggagcaacc
     8641 tgatcgagcg gcaggacccc ttcgggatga agaacatccg ggcgctcagg gataccgcgc
     8701 gcgacggagg gggattcgtc gtctttgtct ggccgaaccc ggacagggag aatcgcgaag
     8761 agctgaagat tggttacgtc ctccccgtcg acgagacctg gtgggtcggc tcaggggtct
     8821 acctctccga gatcaccggt gtggacgctt ctttaccctc tccctgaggg tctccctttc
     8881 ccccccgggc atggtcccct ccaggtcaga gatccgctgc tctacaacgg caacgcatca
     8941 gagttcacga agaacttcag gggcagggcc attgtcgaga tccggaaagc atagcctccc
     9001 gcagcagggc tcgcacgggc agaccaaaaa agcggtcagg gggttggcgc ggcctccacg
     9061 gccgcctctt cctcctcgac gttctcgctg tcccagacga gcgcactcgc catctcggtc
     9121 atgatcaccc gtgaaccggg cggaacccgg atcgcatcgc cgatgtcctc gggggctcct
     9181 gcccagacga cctggtgcct gacatccccg ccgggcagca gcactacgac gctgtaggtg
     9241 ttcataccct ctcccggggc attctcctct tccggggcga cccggtagca tgtaaacgtg
     9301 accggccccg gttctcccga cttcctctct tctgttccac ctttcatctt aattgggcaa
     9361 ttcctcccca tccttcaggg tggggaggaa ttgccccctc cgtgagtatt ttgtagtctg
     9421 aggtcgatac ttcggttact ggcccactga cgccgactcc tgaagaggat gttttaatta
     9481 caccacaatt tgcgtatgat gtgtgccgcg gggagcgata caagtagccg gtgattccgt
     9541 ggttccacgg ataatcggta gaggccgtgt caaccggcct gcacgaataa ggctggataa
     9601 agcaaccttc gggcgaatta aattgcatcg tcgtagtctc catgagattc ccggtgcaag
     9661 cccctccctt tagggagggg tagttgacat gcattcctct cccgtccgaa catgcgcggc
     9721 cccccggagg tctccgcggc catccgctca cggcggagag agccgtcttt atcccccggt
     9781 gacccggcgc cacaccggag agccacctcc tctggatccc ctcttaataa taccttgccc
     9841 cgggcgcacg ttctgcaaga aagatacggt gcatcggaac aggtgtgtta accgcccggg
     9901 agagggccgg atggtctgag aagatgcggc tccggtcgcc aaaaaagtat tctgcgggag
     9961 cccgggatag atcgggtctt cacgccacgt ggatgtactg ctcgaccagt tcccggatcg
    10021 cttcgttatc gccgtagatc ctctcgttca acccccggtc gccgaaggcc ttcagggcgg
    10081 cgatccggct gtcgatcatc cccgccggga agatgccatc cttctcgaag atcgcgcgct
    10141 ttgctgcgag cgccccggcc gactcgacac aggaggcggg gaggtgttcg agctctgcga
    10201 gacgctcctt aaactcgggc cggaagatgt ttccgctgac gtagagcttc tcggccgcct
    10261 cgagtgcccc gggcataccg ataccgtgca gcgacgcgac aacgaggccg gcgacggtca
    10321 ggtaggggtc ggccgaaccg tcggcgaccc ggtactcgaa cgtctgcttc gacgaccgtt
    10381 catcgcggtg gcggttctca cagggattgg catcgtgtgt cataccttcc gcgccgatcc
    10441 agccgagagg cacgcggacc acgaccgagc gattgcggtc gccccagcag atccgtgtcg
    10501 gcgcctcctg gtgggggaca aggcggaggt aggaggtcgg gatggtgttg ccgaaggccg
    10561 tcagggcatc ggagacgtcg agtatgccgg cgatcatctt ccgggcaagc gggcttaacc
    10621 tgccgccctc gaccatcagg ttctggccgt ccttctcggc gagcatatgg aagtgcatgc
    10681 cgctcccggc cttgccgacg gtgatcttcg gggcgaagct gatctcgacg ccgtactggt
    10741 tgccgagcat ccggaggatc cacttggcga tgatcacctg ctcgaccgcc tgctccaccg
    10801 gcatcggcag gaactcgatc tcgtgctgtt cgtagtaggt gtcgccggcg aagaaacacc
    10861 cgacctccga gtggccgtac ttgatcatgc cgccggcccg ggcgatgagg cgcatcgcct
    10921 catcgcgcag gtccgcaaac ttgacgaagg ggtcggccga gtggtagccc ttctggtcgc
    10981 ggcccgggta gagatccccc cgcggactga cgacgtaata ctcgagttcg ccaagggtct
    11041 tgaaggtgca cccggttcgg cgggtgaact cttcgtctgc cttgcggagc acgtactcgg
    11101 gcgaactctc gagcggtttg ccgtcgctgt cgtagaagga gcagagcagt tcaagtgtcg
    11161 gcacctcggc gaagggattg acaaatgccg tgcggtagcg cgggatcacg tagaggtcgc
    11221 tgctccccgc ctcgatgaac gagaagagat tgctgccgtc gacgcgttcg ccgtctgaga
    11281 gtatggtgtc gaggtgttcc tttgaggaga tgacgaagtt gagggtttta agcttgccgt
    11341 cttcggcggc gtagcggaag ttgaccatct cgatccggtt cgcctcacag aaccggatga
    11401 tatcctccct ggtaaactcc gccggatcct tcttgagaaa acagaccaga tcgcttgggt
    11461 tcatcaggat cagagaatct ctcatatttt tttaacgttg atctcaggta tataaaaagc
    11521 gtactctcca cctcatacca cctcgatcgg gagccggagg cttccggcgt tcgggaagat
    11581 ttccgggcct cacccggaac tgcggcaggt gcgtatcccg ggtgcggggg tacggacagc
    11641 cccgggaacg cacccgctct gtgccgcgcg aggagcggca ccgatccgcc cgcgggattc
    11701 acggaggccc ggcacccggt gcggatgaat cccccggttt cacgcgagac ctgccgttat
    11761 ctctccaccc gttacccggc ggtcggggcc ttcgggatgg cggcaccttc cccggcgtcc
    11821 cggggaccgg ccaccttcgc gagcaccgag aggccgagcc gggcgttctc cgccatatac
    11881 ccggacgaga catggtcgat ggtatcctcc ggagtatggg ccgggccgtg ctcctcagaa
    11941 tgcgtcatca gggccgggaa ccccagggcc tggaaagatg tatggtctga ttcgcaggcg
    12001 tgcctgttcc gggtcagggt gaagtttgtg ccgtagaggg tgttgtgatc cgccatgagc
    12061 gccgcgatat ccgcggatcc gctgtcgtac atgatatcga ggatgaagcg gttctccggg
    12121 tcgtagcagg ccgagtcgta gttgaggtag agcaccaccg ggactcccgt atccatggca
    12181 tgcgttgcat agtcgatgct gccgtatgag ccgacctctt cggcgttcca gaacgcgaac
    12241 tgcaccgtcc ggtcaaacga atgcccgctc atcacccggg caagttccat gacgatcgcg
    12301 actccgcatg cgttgtcggt cgccccgggc gcccgggccg ggtccgatga cgtgctgtcg
    12361 tagtgcgctc ccacgatgac gagctcgctt gaagcgttgc cgcttcccgg gagcgtggca
    12421 acgacgttgt tgagttcgcc cccctgaaac tccacatcga gcccggggat gccgtcaagc
    12481 cgtgcgtaaa ggtactcgcc cgcttcccgg ttcccctccg acccgaacac ccgggtcggg
    12541 atcgcctgca ggtcgtatgt cgtgttccag agttcggatt cgttgacctc cgcgatgatc
    12601 ttcacgatgg caggatcggg ttgttcgcgc gccgggggtg cgccggtgag gcccaccgga
    12661 acgatcgtga ggaagagagc caggcaggtg agcagcaggg cacaggggag caccatccgg
    12721 gggcgggcat gccggtcagg gtctttgtcg cacatacgat cacatcccat gagagatacg
    12781 cagaaggcgt tgcatggtga gcgatgacac atataccttc gccagggtgg cgggagggtt
    12841 atggtacgag gaggggccgg attatcccgg acaacgagag gtatatcacc gggcagtttt
    12901 tgaggatccc ccgagattcc tcccgaatcc cggaaccaga ggtatatacc gtatgacgtt
    12961 gactgatgcg tatactcgcc ggaaccaggc catcaccgga gcgcacaagt atgcccgacc
    13021 gaccatcagc aggcgacgag tcgaccctct tacgatggat gcggcttgag atcggcagga
    13081 tcaatgacgg tatcgtctca gagcgcaaac gcctctccag gcttctttca gaagagcgtc
    13141 cttcgtccgt cacgaagggc ggcagcgagt acgggtttga ccgggatatc ctccaccgtc
    13201 tggagcaggc cctccccgga gaactgcaga gacgactccg gctccctatc ctcttctact
    13261 tcgattccac ggtcccggat agcctcttcc ttgcggatga ggccgcgatg caggccctcc
    13321 agagcctcgg agagatcagc cccctccgga ggatgcaggg cgggaggctc tggatagcaa
    13381 aacctctcgt ctacgccatc atgaacaggt acccgacggt gatgcaggtc gtcatggggt
    13441 aaatagcctc tctccggcag ccgccaccac ggcggcccgc cctcagtggc gcggccgacg
    13501 gtacggtatg cggccgccga ccgcctcatc tcccgaacgt ctgccggaat cgctcgagca
    13561 ccggacgggc gacctcatcc atccgccggt caaagtagtc cacccactcc gcgacgaccg
    13621 caagctgccc ctgctgcctg tcaaggtccg cctgcaggtg gtcgactgca tcctggagcc
    13681 gggcgacctc ctccctctcc cgctcaagtt catccgtcag gcgtgctatc cagcgttcct
    13741 gccgggccag cgtgtcctgt atcaccggga ggtcgaacac cagccccggc gggagcgccg
    13801 cgccggttct gccgacctct tccgccggct cctcctgcgc gcccgatctc ctgccggtgc
    13861 cgacctcttc agcgatctcc cccggcgtga ggcctttccc ggagagttcc aggatctcac
    13921 ggatgattgc gaccgccttc tcggggtaga gcctgaccgg gccgatggtc cggtaggaga
    13981 agaggctttc gtaggtccga acgagcgtcc ggacatcctg ctcggagatc ccggtgaggt
    14041 gcgcgatatc gcctatcttg agttctttct cgctcatggt gccaccctcc gcgcccgccc
    14101 tcccgcgagc gggagccctt ccatacttct ttgttctcat caggtatagg agcgtaacgg
    14161 atcgtgggat tttatcgtgc cgccgcaaaa gttactggta ccatgaaccg gggaagagaa
    14221 ggggacgtcc cggaaggtac aaccgtcatc cggaaggctg tgcggtctgt ccgcctccag
    14281 gtgcggccgg acgggaccct gcgggtggtc gctcctccgt cgttcgatgt cggaggattt
    14341 ctgcagcgca acgcggcctg gatcgatgag cggcgcgagg agttcgaccg gatggccgcc
    14401 gaagggcgcg ggaaggagga ccaactcctc ctgcacggaa ggttctaccg cccggtcccc
    14461 ggcgcccgat tttcaatcga cgagacccgg gagaccgtcg cgttcccgtc cgtctccgcc
    14521 ctcaggagag acctctccgg tatgctgaag gaggagaccc tggtccggct caagacctac
    14581 ccggatctta ccggcccctg gtcggggcgg atcgcggtaa aactccagac gacacggtgg
    14641 gggagctgct cgtcgcttgg caacctgaac atcaaccttc gcgccgtagc cctccccgat
    14701 accttgcggg agtacatcgt cgtccacgag gccgcccacc tccgcgagcg gaaccattcg
    14761 aaacgtttct ggctcctcgt cggcgaccgt taccccggat accgggcggc ggagagcgaa
    14821 ctccggcgct actgggtcat cctcgaacgg aaccggatct ggggttctct ccaggacgtc
    14881 gggtgagagg tcccggcact ttgctacacg gtttgagacc gccctcaggg gatcgaacca
    14941 tcgactttcc ttcgcgcgag cgagcgggtg ccgatagcga tacggtctct tgcgaaacgg
    15001 ggagggcctg gaccggatgt gagtgatgcc ttttcgcggc ctcgtatctt cgtgagcgac
    15061 aggtccggat cgcgcaggca cactccggtg ctcccgatcc gctgcgcgca gacgagggcg
    15121 gtccggcgct cgaacaggat tggctcagga gtacttccgc cagaacatgg agagcttgcg
    15181 cgactccgcc cagcgttcgt cgagttgatc gatgatcgcg ttgatccgct gcacctgctg
    15241 ccggatcttc tccgtcgccc cggcatcgtc gagcagttcg gcgcgcccta tgatcgcctg
    15301 gagcgggttc ctgatatggt cggcaagcag ggcaaactgc tccatgttcc gctcaatctg
    15361 agcgtaggct ttcgttcgca ggtcctcgac ctctctctgg gcggtgatgt cccgggcgac
    15421 ggtgacgatc cgcgcgacct cgccgcgctc gatgatcggg atccgtatct cccggagggt
    15481 caccgtccga tcgtcgatcc tgaaagaccg ttccgacgtc agcggccgcc cggtccgtat
    15541 gacccgttcg tattgctgcc tcgtggaggg cgggaagaac ggcaggacgg taaagatgtc
    15601 cttcccgacg acatccgtcg caagtccgag ctcctcgcac caggcgatga aggcatcgtt
    15661 tgcaaggagg agggtgagtc cggtatcgac gacgatgacg gcgtcggtga tggcgtcgag
    15721 gacgatctgg tagaggttct ccgactccct gaagaggtac tcggactgct ttcggtcgag
    15781 tgcactcgcg atgatgtgga cggcggcatg gatgagggcg atatcctcgt tcgcgaggcg
    15841 ggtgtcgcca atacaccgat ctagcccgac aaaaccaacg actttcccgt gcaaccggag
    15901 ggggatcatc agcgccgcgg tggtgcagtg ccgcttcagg agatcacgct cctcttcctg
    15961 cgtctgccct tcgcgaggcg ttccatccat gtacaccatc tcgccgttca ggatccggcc
    16021 gatccaccgg gggagattgc ccgagtggat cttctgaaac tccccccgcc ggggtctctg
    16081 ccctggtgcg caccactcgt gcgtcgtacc aaggatcgcc tttgcatcgt tgaagagggc
    16141 gaggtaggcc ctgcatgccc cgcacgcacg gccgagatca cggagcgagt cgtcgatggc
    16201 cggatcgatc tgcgaggagt ctatgaagcg tgcaggcatc gccgcgacgg cacactccag
    16261 gctgcggcgt tggccggggg tctcctccgg tctttgctgc gcggcggcgt caaggcacga
    16321 gaggatgagc caggggtgtc ccgtcccggg atcacgagcg gtgccggccg atatccggac
    16381 gtcaaagagc gacccgtctc cccgccgggc gacaaccgtc ccggcccacc gaccggtaga
    16441 gagggcctgg ccgatccagt ccggtctctc cccccgggag ggccagagga tatctacggg
    16501 ttttccgacc acgttctccc ggctatagcc ccagatggcg agcaacggag cgctggcgga
    16561 gacgatgcac ccttcccggt cgattatgca ggttccggag agcgatgtct cgatcacctg
    16621 ctgcaggact gcagcggcaa catccctggt tccggcggcg atgaaggcct cgcctcctcg
    16681 cgagtcctga tcgcccatct atcgccctcg aggttcgccg gtcgcaaaac ccttcaccgt
    16741 atgcggatcc atatcccgat cattccagac ggccccgcgt ggccgcaatc cccccgcccc
    16801 ggggtgggtt gcacgctgcc gctctccggg tctccagtcc ggagcggata ggatgatcaa
    16861 tctctcgcac tatatacttt ttctcatttg agtttcggcg atccacggcc atgatcgtgt
    16921 tcccggccgt ccggtgcacg atcaggcgtc ggagagaact gccggctctc gatcggggag
    16981 acgatcaggc gtctgccccc cacccccgcg agtagctccg gtacatcacc aggagcgcga
    17041 tcccgatcag gaggaagacg gcggccgaga cccacgttcc ggcccctgcc gggatgaggt
    17101 tcacgatata gtcgatcccg agggcgatgg tgaaactgcc gatgataccg ccgatggtga
    17161 tgcaggagag gacctcgatc ttcgtcatgc cgagcatcct cccgaggacg gaaccgatga
    17221 tgctccccga cccgtcgaac gggagactca cgaaggcgat gagtccgaca aagtagagcc
    17281 gctcaagcca cggccgccgg tccagatagg cgcgacccgc cgtcatgaac cgtcggatcc
    17341 gggggccgac gtagggcacg cgcaggacga gcgggaagtt ccaggccatg aagagaccgc
    17401 cggcaaagtc cagaaacgcc agggtgaacg cgatcagcca ccaggggatg ccaagaagga
    17461 tgctgaccgg gatgatcgtc tcccggccga gcggtgggac gatgtaggcg accatcatcc
    17521 cggcgagcag gaaccactgt tccgccggga ggaagagtcc gacgacggca aaaaagatac
    17581 cgcagagcag aagcgggact gagactgtga gcaggttcgc ccggctctca accccgtcgt
    17641 cgccccacgt gccggtctgg tgcatgaatc gccgccccga ctccccggtc actcctcttc
    17701 gccggtggga ggagtgggcc cccgggtctt caggatatgc tcgacgatct ttgaggaact
    17761 cgcaagcagg ccggggtatc ccgatatcct gacgacgtcc gcgtcgagtc cccgttcccg
    17821 gagccttcgc cggaggttct cctcgtcaaa gtgctggttg aacccgagcg tgatgatgtc
    17881 gggtttcagc tctgcgatcg gcctgaacat atcgtggatg tcgccgagga gtgcacgatc
    17941 gaccggtttt agcgcccgga ccatctggag cctctggtcc tcggggagga tcggtcttgg
    18001 tttatgcttc acgttcgcat ctcgcgccac gatcacggag agctcatcgc cgaggttccg
    18061 ggactcctca aggtagtaga ggtgcccggg gtggaggata tcaaacgtgc ccgttgcgac
    18121 cacgcggatc atccgaccac ctcgaggcgg gcggggtggc cgtccgcccg gtaaccccgc
    18181 cagtcccgct caccataggg atacccgacg atgatgtgat accttcctac cgtggggaag
    18241 aacctgagat ctgccggcga cggcgagagg acgccgttcg ggtggctatg ggcgctgccg
    18301 gcctggtgga tgtccagcgg gagcatatcg atgaagaacg aggcgctctc ttccccgacg
    18361 atggtgccgg gcaccaggtc gaggtcctga ataatgccgc tcctctctcg caacacggca
    18421 acgaactcat tcggatggtg ttccttcccg agttcaaaga gcatcatgag tagatccctg
    18481 ctgatcccgc gtatagccat tctcctgaat agtttggggg atggagacat aagtcctcgc
    18541 gtccggccgg gacacccggc ccatccggaa cccggagggg aaaaaagaga gggtcgtcta
    18601 cgggatgacc cgccccggcg gcggagccac gggagtctgg ttccccgccg cggtctggtt
    18661 ccccaccgcg gtctggttcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
    18781 cgcggtctgg ttccccaccg cggtctggtt ctccggcagc acgaggcccg gcggcacgag
    18841 gaccgtatcg acgatctgca cgacaccgtt tgaggccgcc atatcggatg cgacgatcgt
    18901 ggcgtttccc accgtgaggt tgccgtcggt cgtgctgacg gtgaggttat ccccggtgag
    18961 tgtcgtgaga accgtccggt ttccggcggc gtttgccttc gccgtcaggt cgttcacggt
    19021 ataggtctct ctcaccacgt ggtgcatgag cagggtatcc agcgtctccg tatcgttgaa
    19081 gagcgctgca acggtctcgt tgccgagagc cgcaaaggcg gcgtcgtccg gcgcaaagac
    19141 tgtatacggc ccgctggcgg caagcaccgt ctgcgcgatg ccggccttcc cgacagcctc
    19201 gccaaatgtg gtcaggaggt cgctcccgct aaccagatcg gcaagcgtcg cgttccccgg
    19261 ctcctctccg gaagttctgt tcccgtccgg ggcgaccgcc attccctccg ggacgacgat
    19321 gacgacgccc tgcatgggcg tccgccgcgt cgaggcgttg agcgtacagc cgtagggata
    19381 ggtccctgta tcggtgaagg tgtagttgaa cgtctcgtta tgctctatcg gcccggagtc
    19441 aaagagcccg tccgtgctcg tgacggtatg gccgatacgg tcgtgattcg tccaggtcac
    19501 cgttgtgttg ctctcgacag tgagtgacgc gggatggtac gaccctccct gtatatcgac
    19561 accggcggtc gccgccaggg cgggcagggc gacgaggccg atgaccaggg ctgttatgaa
    19621 tccgaatatg attggatctc ttctcatgca gtctcaccac acgtatgaac ccgacagggc
    19681 gagcgaccac tccctggcga tggagccgga cgatcccggg tctccggcgg gcgcgtcgct
    19741 caccgctgca cggggtttgg aataattgtc atagtatata atgattatta tatttttgaa
    19801 aattaccgga actctcgacc ggattcgacc atcgccacca ggaaaaaagt acgggatccg
    19861 gttcttcaga acggtccgtc ctgctcgaaa ctccgccgca accggatggc aagttccgcc
    19921 ttgaagagtt ccttgccgag gtaggcggcg tggtcgagcc gggagaggct cccgtttgca
    19981 agaagcgtgt aaaagacgtc ttcccagtgc tttccccgta cggcgcggcc cttgtggacc
    20041 gcgacgatgc aatcctcctc gatgccgatg cggatgcacc cgagaggatc gtagacgatc
    20101 tcgtcgggcg cagggcacgc gtccacgatg ctcccgtact ccagcggggg ttctcgccgc
    20161 cgccgtttct ctttcaggat caagaggtcg agccccaggt ctttggggta tgggcggtcg
    20221 gccatcagcg ccatcatatc ggttgcccgc cgcatctctg ccaccgatcc ccgggtcttg
    20281 tcgctgtgct cgctcgtgaa gatgacggcg gcgccgactt cgtgcgccat acccgcgagc
    20341 agcgcgtttg cacccgtcga gtcggcatcg aggagctcga cgacgtttcc ggctccgaag
    20401 aagagtggat aggggatggc cgggaaaccg gagagcgtat cgacgagccc ggagcccacc
    20461 ggctggagga gggggtcggc gatcaggcgg gagatcccgg cctcccctgc cgccgccagg
    20521 ttctcccgga gcgttcgctc ccgcggcacc acgaccgccg ccgccccggc atccgcgatc
    20581 gccgccccga cgacggggat gtttccctcg tgcagggaga ggaccaggtc ggcgcggggg
    20641 agtgccgcag cgatgaggag agggtcctgg gtgtcgaccg cgagagggcc atcgaggtct
    20701 tcaagggctg caaaacaccg ttcgacgtcc cccggggccg cgtcgaaccc aaatcccagg
    20761 tccacgatat ctgccccttc ggcaaagaac cgccggactt catcggcgat atcgccgcgc
    20821 ctgtgcgcat ccatgatctc cgcgagcacc ttcatccgcg agcctccgcc gatctttacg
    20881 ccccggatga cgaagtcggc gcctgcctcc gcctcccgga gcgccacccg gcggcaggcc
    20941 tccttccggc gcgtctctgt aaggaagtcg tcggcaggga tggtctttga gagccggatc
    21001 cgatcgagaa cgccgaggat cagagcgaga tcggcagcat gccgcgggcc ccggtagacc
    21061 gggacaccgg tctcccgctc gacgccggca aaggatgcgg tgcacatccc cgagacgatc
    21121 gccatatcgt aatcgcggcc agaaagcagt tcgatgaggt cgccgggtgc aaggaacgat
    21181 gcgatatcgc cggtaaccgc cacgtcgatg tcataacggc tgcggaaccg ctcagccgcc
    21241 gccttcacga ttgcagccgt tgccgatccg gtcgggagga ggatgtgcat acacttcact
    21301 tattattcag cgggtgacaa ataatctgat ctcatgctgc ggtgcgatct gcatatccac
    21361 accaggttct caagggacgg agagagcagt gtcgaggaga tcctggagcg ggcggagatg
    21421 gtcggcctcg atgccatcgc gataaccgat cacgatacgg tggaaggcgc cctctatgcc
    21481 ctccggtgcg acacccccct gaccgtgatc ccggggatcg aggtatcgac gcgacagggg
    21541 cacctgctcg ctctcggggt cacgagaccc ctgccggcag gacgtgactt cttcgagacc
    21601 gtcgctcttg cccgcgcccg gggagccctg ctgatcctcc cgcaccccta ccaccgctgg
    21661 cgtcacggcg tcgggaggag gcttgcggcc ggcatcgagg cggtggatgc cgtggaagtc
    21721 ttcaacagcc gttacatcac cgggtcggca aaccggaagg ccgcggtcat tgcacggagg
    21781 ttcggaaagc ccggggttgc cggaagcgat gcccataatg cccgatacgt cgggttcggg
    21841 gtcacgtaca tcacggccaa acccgatgcg gcctccatcc tctccgcgat ccgggagggg
    21901 cggacgatgg cgggagggag gatgaccccg ttgcacacct acacccatca gtccctcagg
    21961 ggcgctttcc ggaagatccg gcgagcgatg caacgatgaa cctggtactt cgcttctcgt
    22021 acttcgggga ccgcttcttc ggttcgcagg tccagcccct ccaccgcacc gtggagggcg
    22081 agttcattgc ggcggcaagg cgcctccgcc tcttcgacga tccgcgagcg gcacatttcg
    22141 ccaccgccgg ccgcaccgac cgcggggtgc atgcccgcgg tcaggtatgt tcgtttgcga
    22201 ccgacatacc ggagcgggct atcgcgaggt taaaccgggt gctcccggag gatatctggt
    22261 gtacggcgtg gactgtggcg ccggaggggt ttcacccccg ctacagcgcc gtatcgagga
    22321 cctatcggta ttacttcttc ccggcgcccg aagagatcgc cgccatgcac gaagcggtgc
    22381 aggagttcgt cgggctgcac gacttctcgg cgttctcccg cgccggcgac aaaaatccct
    22441 tgcgtcggat ccttgcggca cgggtctttg aggagggtcc gtttgccgtc tttgaggtga
    22501 ccggagagag tttcctctgg aatatggtcc gatgcatggc aacggcactc aaacgggtgg
    22561 ggaatggcaa gatcgaccgg ggagctatcg ccttgcttct tgcgtgtccg gcgggcgagg
    22621 ggctcccggc cgcccccccg gaggggttga tcctctggga tgtcgagtac gggttcccct
    22681 ttaagcctct ccctgctgat gagcggagcc gccggtatcc ggtcggccgt catcgctacc
    22741 acatgctcat ggcggagctc tccgcacgcc ttgtgccggg agaaggtccg ccggggtgat
    22801 ctgtgccttc gccggtctcg tgcggcagcc ggcacacacc ataaaggtac cgtatccggc
    22861 aaaaacggag aaacgacaac cgagaaacgg gtccttttca tcaccgctgg taaaaaagga
    22921 tcctgccgca gacagcggat ctcccaaagg aggtgcggca gggtaaatcc ggctttttgc
    22981 ctgccatcgg tgaaggccgg gcttaaactt ttcggatagc accttacacg ataaccccgg
    23041 accgctggaa tcgccggatg aggatctgca accgtcgacc gatatgggat cctgagtcac
    23101 ggcacggagg caggaacgct taattagtcc ggtggagact taagagtact cctgtaagct
    23161 gatttcagct tacttttcca atatgttaag caataaacct taataataac ctattaatat
    23221 atgccagcac agtcccgctt cagtacgtca agaggggaaa gtggatgtac gacaaggatc
    23281 tggttcgggc gagaccaccc gctcaccacg aggggtggtt cgccgaagag acagctgtgc
    23341 cgttaccgca gttcatggat acagagacag acaggtggat cgaacgacat gtgccccaga
    23401 agatcggaga ggggagcccg atggatattc ccgctctccg ttgcgaggtc caggagcagg
    23461 tcaacccgct gaggatgccg acgggccccg gacaccggcg ggtggcaggc gaggtcgccg
    23521 ggcccgcagc cgccccatcc cgtcggtcag ccagggtgag gaccctcgcg gcgatcccct
    23581 gtttcaacga agagatcgcc atcgggtcgg tcgtcctgat ggcccggctg cacgccgacg
    23641 aggtcctggt gatcgatgac ggatgcacgg acggcaccgc aacggtcgcc cgggatgccg
    23701 gtgcgaccgt catatcgcac ggagcgcaga tggggaaggg gcgggggatc aggagcgctc
    23761 ttcgatacgc ggtcgatcac gattatggct gcctggtctt catggacgga gacggtcaac
    23821 acaaccccgg ggagataccc ctcctggtcg agccgatcct caccgatacc gctgatctcg
    23881 tcatcggttt tcggaccttc gaccagatgc ccttttaccg gaggttcggc cgggccgtac
    23941 tcgacgtcgc ctcaagcaac ggcagttcga tcaccgactc ccagtgcggg tttcgtgcgt
    24001 taaacaggac ggccatagat gcgatgctcg atggactgaa gaaagatgat ttttcgaccg
    24061 agtcggagat gctcagaacc gcacgagaga actgtctgcg cgtcggggag gcgccgatca
    24121 actgtaaata cggcggcgat ctcagtacgt ccacgaaaaa tcccatatcc cacgcgatag
    24181 aggtgctcgg atcgatattc tggctcgccg tggagagaaa acctctcctg catatcgggc
    24241 ttcccggact tgccgcggtc ctcgccggca tctttctcct ccttcagttc ttccgggggt
    24301 ttggcgagac gggtcttgtc gcgatcgagc aggggatgct tgcatccctc ttcctgatcc
    24361 ccgggaccgt cgctctcatc gtgggtctga tgcttgctct cattgcacgg ctgcgggagt
    24421 gagggggaga agagatacga tacagaggag gagcgcagaa tgataggaat aatacttgga
    24481 acaaggccgg agatcatcaa gatgtctccg gtcatcaggg aatgcgagag aagggggctc
    24541 gactacttca tccttcatac ggggcagcac tactcctacg agatggaccg gctcttcttt
    24601 gaggaacttg agctcccgga ccccgactac aacctcgacg tgggttccgg gagccaggcc
    24661 ggccagacgg cgaagatcat gaccggtgtc gaggatatcc tcgtaaagga ggcgcccgac
    24721 ctcatcctgg tgcaggggga tacgaacacc gtcatggccg gggcgcttgc cgcatcgaaa
    24781 cttcacgtgc gggtggggca catcgaggcg ggcctccgga gtttcaaccg ccggatgcct
    24841 gaggagatca accggatcct taccgaccat atgtcggatt acctctttgc cccgacggag
    24901 aacgcccgga agaacctcct tgcagagggt attgcagaca ggaggatctg cgtgaccggc
    24961 aacaccgtcg tcgatgcggt ctaccagaac ctcgatatat cgaggaagaa ggtcagcgtc
    25021 ctcaaggatc tcgacctcaa atcccgcgaa tatttcctcg tcaccgccca ccggcaggag
    25081 aacgtggaca accgggcacg gctcaaggag atcctgagag ggcttctagg ggtacaaaag
    25141 gagtttgccc tgccggtcgt cttccccgtc cacccgagga ccgagaagag gatcaaggag
    25201 ctcggtatcg gcatcgacgg attaaacttc accaaaccct tcgggtttct tgagttcctg
    25261 cagttagagt cgcaggcgaa ggccgtgctg accgattccg gcggcgtcca ggaggagacc
    25321 tgtgttctcg gcgttccctg cgcgacgatg cgctacgata cggagcggcc cgagacgctg
    25381 aatgtcggat cgaacgtcct ggtcggtgcc gactcaagga agatccttga aggagtacgc
    25441 tttgcagtca cccggaaacc tggctgggag aacccctatg gggacggtat cgccggaaag
    25501 atgatcgtca tggtctgtgt agcagcgcag tcccaataac cgcacgagga gatgatgtta
    25561 tgaagatctg tgtactggga ttaggacaca tcgggttgcc gaccgcgctg ctgtttgccg
    25621 cccacggggc ggatgtcgtg ggtgtcgatg tgaaacagag cgtggtggat tgcctgaacc
    25681 acggaagcct cccgttcagg gaaccgggac tcgaagatct gtaccgtgag gcagaagaac
    25741 ggttcgccgc aagggccagg cccggaaccg cggatgtctt cctgatcgcc gtgccgacac
    25801 ccctggatca ggcgacaaag gtctccgacc tctcctacgt caagaaagcg gccgactcga
    25861 tcgctcccca ccttgccagg gggaacctgg tcatcctcga atcgaccgtt ccacccggaa
    25921 cgagcgagcg ggtcgtcatc ccccggctcg agaagaaagg catccctatc ggggatttcc
    25981 tctatgctca ctgtcccgag cgggcgatac cggggcggac cctccaggag atgacggaga
    26041 atagccgtat catcggggga tacgaccggg attccaccga ccgggcgacc tctatctacc
    26101 agaccttcgt ccgcgggcag atctaccaga ccgacacgag gacggcggag tttgtcaaac
    26161 tgatggagaa cacctgccgc gacgtgaata tcgcgctcgc aaacgagttc gcccagctcg
    26221 cggaggagtg cgggatcaac gtctgggagg ccatcaccct tgcaaataaa catccccggg
    26281 tctccatcct gaaccccggc ccgggggtgg gagggcattg tatcgccatc gatccctggt
    26341 tcctgaccga aaactccacc cggtgcagga tggtctctac ggcacgagag gtcaacgact
    26401 ccatgccgaa ctacgtgctg cacgtcgttc gcaacctgct tgccggcacc cgggacccga
    26461 cgatcagcat cttcggtgtc gcctacaagg gcaacatcgg cgatacccgg gagagtccgg
    26521 cgttcaagtt catccagctc gccgagaacg agggatacgc catccggtgc cacgacccct
    26581 acgtggggga gttcccgcat ccccttgtga atctcgcgga tgcggcagcc gggagcgact
    26641 gcatcgtcgt actcgccgat cacgactgtt tccgcacgat cgatcccgcg gggcttcgtg
    26701 tacgaacgaa gctcgtcatc gacgcgagga acatactcga ccacgagaag tggaccgatc
    26761 agggttttgc cgtccgtgtg ctgggtgacg gttcatcggt gcagcagttg ccgcccggtg
    26821 aggttatgcc tcccgcagcc ctctactcga cggatgcgat acgaccgatc gcaccctcgt
    26881 taccaccatc catctctctg ctctccccga acgggcgggg ggatttcctc taacccctat
    26941 ccgaaccggt gggaggaggg agcctcatcc atctcgataa acgcaaccag gcatcacgag
    27001 gagttccatg agacgggtat gcatcgtatc acagcacttt ccaccggaga agtcagggaa
    27061 cgcgtcgcgc atctacgata ctgccgtgca cctggcggat ctgggtctcg acgtgaccgt
    27121 gcttgcaccg catcccacgt ttcctacggg ttcgttcccg cgcacatggc gtcgatccag
    27181 cgtacaggag gtcaacggcg tccatgtcgt gcgcctctgg acgtggcagc cggcatcggg
    27241 agatcccggg ttcccgagcc ggatggcgta ttaccttctc ttcccgctcc acgccgcgct
    27301 ctggcttatc tttgcgcaga accggttcga cgtgatcgtc acatccgcac cccctctctt
    27361 caccggtctc ccggggtata tcctgaggcg gacgtcggcg gtaaagtgga tcctcgatat
    27421 ccgcgacctc tggatcgatg cgtcgattgg tctagggttc ctgaaagagg ggagcatatc
    27481 cgagaggttg agccggcggt tcgagcaggc ctgcctttcg cgggcagatt tcgtcggtgt
    27541 cacgacggaa gaactcggcc ggaggatcgc atcccgctac cgtgtcgcgg ctcccatgga
    27601 actgatgccg aacggcgtcg ataccgatct ctttcacccc acgagcggcg gaaaaaagag
    27661 gcagatcata tacgccggca acgtcgggca cgcacaggac ctcgataagg tggccctcgc
    27721 gatcaagtcg atgaacggga cctacgacct gaaatttctc atcgtcggcg acggcgacac
    27781 aaagaagcac ctcgaacagc tggtaaagac cgagaacctg gccgactcga tcatcttcac
    27841 cggcaccctc ccgcgcgagg agatcccgcg actgctctcc gagtcgctcg tggggatggc
    27901 gcccctgaag agacttgcaa acctcgagta cgccgctccc accaaggctt acgaatacat
    27961 ggcctgcggg ctgcccttcg tcggctgcgg ggacggcgag atcgcccatc tcgcgcatga
    28021 gtcgggcgcc ggggtcatcg ccggcaacac gccggaggcg atcgccgcga cgcttgccac
    28081 gcttcttgac gacccgggga ggatggagga gatgggccgc cgggggcggg agtacgttgc
    28141 cgagcactat ggccggagag cggtcgccct gaaactgaag cagcatatcg agaggatgac
    28201 atggacgagc gcctgacgtt gctcctctcc cgggttgccg ggaaggtccg ggagctttgg
    28261 gtcaccgatg gagagaccgc gtacatccgc gacccggtct ttcccgtcat ccggaaccgc
    28321 gtgcacgccg agttctgtaa ggcgatgctc cgttccggcg gcaacacgct cgtaggcccg
    28381 atcttgaact acgtcgcggc ctgccagaac ccggacgggt cctggaacga ggttcacgtc
    28441 aactacaacg agccttcggc cctgatcacc gccttcatcg gcgatgcgct catcgaggcg
    28501 gcggatcggc atcctcacga ggatgcgctc gagcgggccc gggacttcgt cctcgcagcc
    28561 gagaggagcc ccggctgctt cctgaaatct gcaggatata cagccgacca cctgaacgtc
    28621 gacgcctcct gcggggcgtt tcttgcccgg tacgcggagc gctacgacga ccgggaggca
    28681 cgcgcggccg cccggcgtgc ggcggagaac gtcgtctccc accagaggga cggcgtttat
    28741 ccctatgccg tcgataaggg aacctaccct tacgtcttcg acctgccctg catccactac
    28801 cagggagtga cgatctacta cctggcaaag acgaacgacg tgctctgcga cgaccggatc
    28861 gaccggagca tcgcggaggc ggcccggtgg ctcgcggccg cccagcgccc cgacggccgg
    28921 ttcgactggt cgggaagcgg gctctccttt gcctgctacc tcagcggggc gtacgcgttc
    28981 gcccaggcct cgttcaccta cgcatcccgc ctcgatgacc gctaccggga gcacgccgat
    29041 cgctctcttg gctggctcga tgcgaacgtc aggggtcttg cgccccggtg ggaaccgggc
    29101 tcctggggcg atctccccct ctcggtcgcg gcggcggtaa aatccgcgtc gctcggggga
    29161 tacccgctcc ggcaccgggc gttccggttc ggctacggtg tctaccggga ggtcgcccgc
    29221 cggcggtata gcgcgaccgc ggagacaaga gcattcgagg cgctctcggg gctgctcggt
    29281 atcaggtcgt cgaccgtgga gccgtcgaag aactttcccg acctcttcat gacatcggag
    29341 atgctcgatt gcctcagtca atcgatgata tggggaaaaa acacatgaaa caggtattac
    29401 tcgatctgca atcagggtcc atccgggtgg aggacgttcc cgttcccgcc gtaacgagag
    29461 gggtcgtcgt cgagaacgcc tactccctca tcagcgccgg cacggagtcg tcgctgatca
    29521 acc
//
LOCUS       DAOSEE010000009        27905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_795_length_27905_cov_269.639856, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000009 DAOSEE010000000
VERSION     DAOSEE010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 27905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27905
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_795_length_27905_cov_269.639856"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="PLI76_01450"
     CDS             complement(<1..805)
                     /locus_tag="PLI76_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOI60725.1"
                     /translation="MIGPELIAIAIFLFTYALIIDERIHRAVAAMLGASVIVFLHIVP
                     WEKIPVYVDLGTIFLLMGMMIIVNTARGSGLFEYIAIKTAKLAKGSPMRVLLLFSLVT
                     AVISAFLDNVTTVLLITPMLLYIAGVMRITPLPFLIAEIFASNVGGAATLIGDPPNIM
                     IGSAAGLTFNEFLVNLAPIMVVNIVVVMGMLALIYRKELHVSPGEKGAIEKTFAELNE
                     QEAIRDISLFKKSVAVIALVIGMFFIHDLLGLEPALVALIGASILLFWSR"
     gene            complement(1001..3712)
                     /locus_tag="PLI76_01455"
     CDS             complement(1001..3712)
                     /locus_tag="PLI76_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOI60726.1"
                     /translation="MIVPTGDREEALPAEWYTLSSDEVEQTLGTGPHGLSREEAEARL
                     QRYGANVLREEERETRLQVFLRQFASILIIILIVAAVVSFFVGEPQDAAAILIIVVLN
                     ALLGYSQEWQAGEAIEALRQMLVQKAVVIRDGKQQEIDASGIVPGDLVALEMGDRVPA
                     DLSLVEATSLQVDEAPLTGESSPVDKATGPLTPETPLAERSNMVFAGTTVVNGWGRGV
                     AVATGMRTEFGLIAGLSQRVRAEATPLARQMDVLGRDIGIIAVGIAVLAIGVGFLQQR
                     EFLEMFLIGVSLAVAVIPEGLPAVVTLTLAIGIKNMMRRNCLIRRLPASETLGAVSVI
                     CTDKTGTLTRNEMTVVRVRTPESEVAVTGTGYAPEGEFLRDGSAIDPLADPDLRQFLR
                     AALLCNHATFAGDEAGEWRIFGTPTEGALVVAAAKAGLSRDDAPEAVREFSFNSTRKR
                     MTIIYREGDGDVAYVKGAPEVLLDLSSRLFRDGAIVPLTDDLRDALQQELCGYASRGL
                     RVLGAAYRPLPGDVPRTAEAVEKDLIFLGFAGIVDPPRPEAAEAIRLCRSAGIDVIMI
                     TGDNPLTASAVAQDLGLSSEGALTGADLEAMTDDELARRLRTTKVLSRVTAEHKLRVI
                     DILSRDREVIAMTGDGVNDAPALKKASIGIAMGIKGTDVAKESSDMVLVDDNFASIVA
                     GVEEGRREYDNIARFTRYLLSSNVGEIVAIVGGLLMGLPLILIPVQILWINLITDGPT
                     ALALGLEPAEKDVMQRRPRNPDESILTRSALLVILVIGAWLGLLTLYAFSGFYEVDID
                     RARTMAFTGLIIFEMYNVLNFRSFRFPLHRIGFFSNPTLILAILGSLGLQALVVYVPI
                     LQEFLGTAPLTLADWGLLFLLGLPILLAGEIYKILRLRSEEKGRTPA"
     gene            3858..4409
                     /locus_tag="PLI76_01460"
     CDS             3858..4409
                     /locus_tag="PLI76_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HOI60727.1"
                     /translation="MSRIAVLITDMFEDVEYTEPAAAFREAGHDLVHVGLSAGETVHG
                     KKGEAEVTIDRAVSDVSVDSFDALFIPGGYSPDKLRAHDAPVKFVQEFVQSGKPVLLI
                     CHAPQLLITAQALSGRRITGWRSVAQDIKNAGAEYVDQEVVVDGNLVSSRQPDDIPAF
                     VRASLEKLAEAGGKVEAAAVRQR"
     gene            4477..5676
                     /locus_tag="PLI76_01465"
     CDS             4477..5676
                     /locus_tag="PLI76_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOI60728.1"
                     /translation="MKPAGSTWSRPAVIVLTAVLIVLLGAIARVLTGIDAAYTDLLYL
                     VLIPTAYWYRRHAVYPGILVAVLHIVATYIVTGHVGAETLVRAGMLIVIPYVLGYFFE
                     VVGRRTEEKHFHAGESGPVACDRSTKRLIARLSSRDPDTRYEAAGCLGNAGDPAAVLP
                     LAALLRDPESGVRWKAAEALGRLGSPAVEPLAGSLQSEDVDVRWMAAVALGDIGDPAA
                     VPALAKALDDDDAYVRSRAALALAAIGEPAREMLITGLSTGNARVRGSAALALGRLGG
                     ESATGALIDALRDPDEEVRHRAAVALGDIGEPAVPDLLSATRADEADVRRGVTAAFGQ
                     IGKPAVAPLIAALDDDDWRIRRGAAAALGDIDDPGSVDALIRALDDAREEVREQARKA
                     LGSIRKT"
     gene            5703..6224
                     /locus_tag="PLI76_01470"
     CDS             5703..6224
                     /locus_tag="PLI76_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOI60729.1"
                     /translation="MTDETRIDELVADLRRGPLAVRRAATAELGASGAVAVEPLIAAM
                     LDGDNDLRWYAARALVQIGEAAIDPLLAAMRAGENRDFRRYAMAALAGIGEAAVEPLI
                     DIIECADPALQPFAAMALMKIGEPAVEPLLRLMQSPDPKTQERAALLLCRMGETGAEP
                     LTGALETDRSGKR"
     gene            6407..10270
                     /locus_tag="PLI76_01475"
     CDS             6407..10270
                     /locus_tag="PLI76_01475"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase II large subunit"
                     /protein_id="HOI60730.1"
                     /translation="MEVSPAIERYFENLAAGLDSAMALAAAARARGLDPRTEIEIPVA
                     SDLADRVEALLGYPGIAARIRELEAEMSREEAALRIGDDFAARMFGETTTEEILDHAI
                     RGAMALLTEGVVAAPTEGIAKVSLGKNDDGTDYLKIYYAGPIRSAGGTAQALSVLVGD
                     YVRQALGINRYVPRPEEVERYIEEIRQYNNIMSLQYLPSEKELRMIIENCPVCIDGEP
                     TEQQEVSGYRNLERVETNTVRGGMALVVAEGLALKAPKIVKNVKKMKMAGWDWLEEMI
                     GGGGAAKSDDDDKGAAVKPKDKYLRDLIGGRPVFSYPMRKGGFRLRLGRSRNTGFAAA
                     GLNPATMHILGDFLAVGTQMKIERPGKAAGIVPVDSIQGPTVKLRSGEVRRVDDAAEA
                     RRIAGQVDEILDDGEILISFGEFMENNHPLMPPCYCEEWWRLEGGPRHPASELEAIEF
                     ALDGIPLHPDYTYLWDDVAPADIALLADRISAGGRIEGGVLTLPDTPEAKAILEELLV
                     PHRLSGDRIAIPGYLVLLACLGLTLHLDKRPAWENAPLENSLDLVMHLSGFSIRSRAG
                     TRVGGRMGRPGKSKQREMKPPPHSLFPIGDEGGSRRSFQAACASRPRSNTDGGVIEVE
                     VGERRCPSCGTFTYKNLCDCGAHTRPVLRCPKCDQEITKDMCPRCSVATVCLQKVSIN
                     VRIEYAAALANLGVRESAIPLLKGVKGLISRERPVEPIEKGILRALQNLYVFKDGTVR
                     YDMIDLPLTHFRPDEIGVPVERLRELGYTHDIYGRELVETDQVLELRHQDILVSEDCG
                     EWLVRVAKFIDDLLVRVYGLEPFYRAEQPLDLVGHLLMGLAPHTSAGVLVRLIGFSKA
                     GVGYGHPFFHAAKRRNCFAGDTEIVVSDGRQWVTVPIRQFVVENFDLSKPGLDRTGTF
                     YSDPRQPFYARSIDPQGLVGVKKITSVSIHRAPAHLIRFVTGRGKVLSVTPDHAMLVW
                     DTDYLRKIRALEVKIGDRVPVEEGGLVISDVVVSRETVQALDDRVYCLTVAENHTLVA
                     NGIFCGQCDGDEDCVMLLLDGLINFSRAYLPETRGGTMDAPLVLTTRIDPAEVDKESL
                     NVDVCDHYPLEVYNGCLAYAHPKDLDKYVDRVERRLGTPAQVEGFFFTHPTSDISAGP
                     LESTYTKLGTMLEKLDAELDLAKKIRAVDADDVAERVLNTHFIRDLQGNLNAFSKQKV
                     RCMKCNAKYRRMPLAGKCTRCGGNVIPTVHEGSVKKYLDMSRNICETYAITEYTKQRV
                     EVLFMQIESTFGEPPERQLGLADFM"
     gene            10277..11104
                     /locus_tag="PLI76_01480"
     CDS             10277..11104
                     /locus_tag="PLI76_01480"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOI60731.1"
                     /translation="MEAREVLSAIRAGIPIRGDRRRPEIEPLPEIRAYRLTTPATRAD
                     RTILFFHAGGFTSGSTADSLDLSARLCDAARSEVVSVDYRLAPAHPFPAAVEDCLSAY
                     RYLLHEGYSPERVVPAGLSAGGTLALSMLLSARGAGLPMPAAGVLLSPAVDMLFLGES
                     VLLNRETDWLAPEQFAAMREDYLAGRDPCDPLASPLYADLSGLPPLLVQAGGGEMLID
                     GIEAFVSTARLQGVPVAFDCTEGMFHCWQAFAGVLPEGAMAVERIGAFVRRHVPDEG"
     gene            11157..11239
                     /locus_tag="PLI76_01485"
     tRNA            11157..11239
                     /locus_tag="PLI76_01485"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11191..11193,aa:Leu,seq:caa)
     gene            11307..11558
                     /locus_tag="PLI76_01490"
     CDS             11307..11558
                     /locus_tag="PLI76_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOI60732.1"
                     /translation="MSETHLRHPVLMEQNEDGGYIVMVPSLSGCVSEGSTRDEALAHI
                     EEAIAGYVEVARETRQTSITSGGGSLLSGEGRHTGFRAV"
     gene            complement(11641..11778)
                     /locus_tag="PLI76_01495"
     CDS             complement(11641..11778)
                     /locus_tag="PLI76_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60733.1"
                     /translation="MPTSKKQMEKLNKAKKVKAEELAQQAAAGSQAAKKKLKKLEKKI
                     K"
     gene            complement(11976..12404)
                     /locus_tag="PLI76_01500"
     CDS             complement(11976..12404)
                     /locus_tag="PLI76_01500"
                     /inference="COORDINATES: protein motif:HMM:NF016220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HOI60734.1"
                     /translation="MTSFVESEVEETVIARFADPGYTVLSGPAIVPGEADEERTDYRQ
                     PFHPRQVRNTLRRINPHLPESAVDEAARKLTTPSSASLIEKNHTFHRYLTDGIPVEYG
                     ENRTIRHGLAKVIDLEEPGKNDFGPRLTKDASRFPAGNLP"
     gene            complement(12401..13681)
                     /locus_tag="PLI76_01505"
     CDS             complement(12401..13681)
                     /locus_tag="PLI76_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOI60735.1"
                     /translation="MKLPRTPEYSPADVEKAWEYADTNDVIDAVFKYNKKYLHWEELK
                     WRELPVDPLYVWILMKTFRERNMKYVPFDYLQLKYNLLDDFLENLHFLDKSAAGSISS
                     GLDSLPQDRNQFVVNSLMEEAIASSQLEGAAVTRTIAKKMLREKRKPTTKDERMILNN
                     YATMKRITRIKDEDLTRELILEIHKTITNDTLEDPGDEGRFRDNNEIRVFDMRGNVLH
                     TPPDFALIEELIGKLCAFANNDGGQFIHPVIKGIILHFLIGYIHPFNDGNGRTARSIF
                     YWYMLRKDYWLFEYMAVSRAIKDTKGQYSRAYQYTESDENDLTYFIRYNLTAVKTAVG
                     SIQRYIEKKQKESKQALAILQQSEGLSLRQAEIIKSLIKKPDKPVTIKEIAETYRVAY
                     ATARKDLFHLEELGYLAKQRSGKEYIFIFKGLKE"
     gene            complement(13861..14625)
                     /locus_tag="PLI76_01510"
     CDS             complement(13861..14625)
                     /locus_tag="PLI76_01510"
                     /inference="COORDINATES: protein motif:HMM:NF020436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1829 domain-containing protein"
                     /protein_id="HOI60736.1"
                     /translation="MINEIQGLVNEYARWLREKTQVRQVDEWVEITTPYLDRHNDYLQ
                     IYTRRRNGDYILTDAGYILQDLELSGCKLDSPKRRALLMITLNGFGVKLNKDRLEVYT
                     SVASFAPSKHNLIQAMLAVNDLFGLATPMTTSLFYEDVVTWLDVHDIRYASNVKFTGQ
                     SGFDHLFDFIIPKSRNQPERIIKTINNPSRNTAQAVAFAWIDIKEARPPDSKAYAILN
                     DSERPVPGNVLDALNNYGVKTVSWNNREAVVKELAT"
     gene            complement(14622..15068)
                     /locus_tag="PLI76_01515"
     CDS             complement(14622..15068)
                     /locus_tag="PLI76_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60737.1"
                     /translation="MSDISLDQATADVLIAIPKVRANNDASRYPGAGGYLTLPLLSSD
                     GRESFHLDIRRGSIDLLKGTYQNRARQVVTLVRLDFGGAPHRNPDGAEVSSPHLHIYR
                     EGYGDKWAVEVPSDRFSHIEDPWQTLQDFMQFCNIVDPPDIQRGIE"
     gene            complement(15081..15395)
                     /locus_tag="PLI76_01520"
     CDS             complement(15081..15395)
                     /locus_tag="PLI76_01520"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF012982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase"
                     /protein_id="HOI60738.1"
                     /translation="MDAPDPAYIPQKIIDTALEFIDASLRTGNQVLVHCNEGCSRSPA
                     IGLLYLVKYTDLLPIDDFIQAEEVFRSIYPSYNPKNGIRGYMTTNWSRYASRSDQNSK
                     SG"
     gene            15294..15614
                     /locus_tag="PLI76_01525"
     CDS             15294..15614
                     /locus_tag="PLI76_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60739.1"
                     /translation="MNKNLVAGSQAGVDELQCRIDDLLWDVGWVRSIHEVQDKPFSPS
                     RDEVFRVIFWGSSTPVAQSLTMVWLLAGMNDAPSCFMSDFKFVIVLVADVQVRVNLDH
                     GGTA"
     gene            complement(16189..17256)
                     /locus_tag="PLI76_01530"
     CDS             complement(16189..17256)
                     /locus_tag="PLI76_01530"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOI60740.1"
                     /translation="MKKTDRYSAHEEKRAPFGRILLCSLIACMLLLAIPVGAAEAVSF
                     PDTDLEAAVRDALDKPRGDITTDDMAGLGALDAEDKGISDLSGLEHAVNLRGLDLDSN
                     EISDLGPLAGLTDLQDLDLDSNEISDLSPLVNLTDLRNLELDSNEISDLGPLAGLKNL
                     RTLDLDSNEISDLSPLVNLTSLWALDLDSNKISDIGPLANLTNLRDLDLESNEISNIS
                     PLAGLKNLRELDIDGNQISDISPLANLTNLRELDISSNQVSDLTPLANLTNLRDLDLD
                     SNKVSDLGPLAGLKNLKKIDLDSNEIRDIGPLVRSELGTGATVELRHNFLDLTPGSAA
                     MKDIEALEKRGVDVSYIPQRR"
     gene            17833..18264
                     /locus_tag="PLI76_01535"
     CDS             17833..18264
                     /locus_tag="PLI76_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOI60741.1"
                     /translation="MRDVHRVIEGRGNYTFIVHNHYTGDAQEVRVDPDRIALFEDKSS
                     IEGLPNACFFLRFDGEKAWCTVHLTRPALCREYCCRLLILDPQGRLAGRVTYQRALVP
                     DTDEFSRLWEQVRPALDDLSGVEWDDALIRILAPAGYRVRR"
     gene            complement(18326..19585)
                     /locus_tag="PLI76_01540"
     CDS             complement(18326..19585)
                     /locus_tag="PLI76_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60742.1"
                     /translation="MRLPYGDRFGRKKEAERPAIPPDEFSQQLACSRAEAEKILAAAE
                     GDLATLTGMRWSLEHTSLILGAAILVNLALLFAYTREYSLYWIMASFIFLMVNPFLLL
                     LPTDRGDLRSYVRYFIDLKDRERKSLEEIISWKSLEKGAAIPADAMEALRRLTHQRKS
                     LYELGWNLFFINCQPLAPGFLVLFALSSVFALTGWLVNGDFASYSAVIVVIQSAAIIV
                     FYGAIVYVQPYSRGFFAGMLGMQSRIRERYNEAWSQGLRYALTVAVVTTVAGVIFIAA
                     IVLPGVTYSSFASAEADIELGAGMFALIFLTQLIVVRHLQGGYSRTLVHSLLSSRIEM
                     IREEVLPAIADLVPAPLCATATSGMAEDLKRITLDLARHRALKIDYVSLFGYFPVCMV
                     TPDIGAIMRVAGVVDGDRERTGTAGTA"
     gene            complement(19689..20168)
                     /locus_tag="PLI76_01545"
     CDS             complement(19689..20168)
                     /locus_tag="PLI76_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOI60743.1"
                     /translation="MQNGPAPDTSAPEDGSSVERGNYTSNETLVAFVESAAAYVRENG
                     KEKALAEFSDLNGSFIQGELYIYAYDFNGTTLAHPVNPEKIGVNRLNETEGDVGIFLR
                     KMNDAVRYGDGFGRIAYINPMRDRVLESKLAYGVQIDETWWLGSGVYTGSVDLDTSG"
     gene            complement(20352..20846)
                     /locus_tag="PLI76_01550"
     CDS             complement(20352..20846)
                     /locus_tag="PLI76_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60744.1"
                     /translation="MPHFDLFFKTEELRRRLEPHLHLIPPYFRFTVRVGTPEVRYFDP
                     KDPMWKGFPFPVPERTVYVFDDAIPARALGGGMDMRASVRVTRGDTDDEAIVLRIWHE
                     ILHAIGQPADDMVRRAAEWQSVSERLVWAAWQSLSRPVDVPFWHRKFYAWLTERAESG
                     AGGR"
     gene            complement(21317..21646)
                     /locus_tag="PLI76_01555"
     CDS             complement(21317..21646)
                     /locus_tag="PLI76_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60745.1"
                     /translation="MELCTAYANPLFMESTIRLNLTRVLEVTGELKHFLDLGAIRLQA
                     AGQLSQEASEALIFAMADELEDHIRAMRDRQGTATIRDIRTWIRAWIDEQEAALGVKP
                     PGNGDRG"
     gene            21852..22343
                     /locus_tag="PLI76_01560"
     CDS             21852..22343
                     /locus_tag="PLI76_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HOI60746.1"
                     /translation="MEVKKVQERSGTVLERRTRTTGEEWHMSADDIMNKKVINSRGDD
                     IGSISDMRVSFPEGRVKYLVLKHGGTLGMGAKHFAIPPEAVIYRSGDDNFVVNIDKQQ
                     LDDRPGFDKDNWPQEADTSLIQSSGAITPPTREEAEAMRREVPPPAEVVKKETIETRP
                     RQK"
     gene            22519..23742
                     /locus_tag="PLI76_01565"
     CDS             22519..23742
                     /locus_tag="PLI76_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOI60747.1"
                     /translation="MDLRVWQVVFIAVIAIVFTVAYLSLYAQLNKTIWGEDSLLVVTP
                     WAIPAAVVFFSLLVGLSRKYLHAPDVIHEGFADSLKGGEGGKPDHRTFPGALLSSLFS
                     LLSGASIGPEGTITLLLMYVSSFVRKRLRIESPSAALGFDVAALASAFNGVIGSVLFT
                     GVFATEFQVGGNKNALRLLTWNLLAGTIGFGFYLLIGFPSFARSIPFEPMGEFLPVYI
                     LYAVALGIAGSLLAVFAGVSMQAAEKIMDRVFGGAVVTRVLAAGVVVAAVGYAIPELL
                     FSGEAQIHGIITDPARFGAVMLLGMAIAKILLLALSFKSGYIGGPLFPIIFSSTLVGL
                     ALSLVFPGIPVAILVLCIEAAALTFALGAPLTSILLVVVVGTADQSMIVLLTLSAVTA
                     MLIAARVKERMGERA"
     gene            23841..24158
                     /locus_tag="PLI76_01570"
     CDS             23841..24158
                     /locus_tag="PLI76_01570"
                     /inference="COORDINATES: protein motif:HMM:NF023174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HOI60748.1"
                     /translation="MQFMNVFPREPGKTAEIMETRAREEIPEGVTVIDEWVDSRGTQV
                     FPVIETDDPVALMKLGFPWSGLGYGDAPGYGVKRSVDYSRGMRRGAPYAPSRSCPGLT
                     GTT"
     gene            complement(24113..24901)
                     /locus_tag="PLI76_01575"
     CDS             complement(24113..24901)
                     /locus_tag="PLI76_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI60749.1"
                     /translation="MALLSVEDLSFAYRDREILSRISFPVDTGEVLGLVGPNGCGKTT
                     LIRCVDGMLTPGSGRVLLHGRDLRGMHRRDIARAMAYVPQSAGNQTASTVFETVLMGR
                     RPYLNWTVSPADEEKVVEALAILDLGGLALRKIRELSGGERQRVMIARALVQETGVVL
                     LDEPTSNLDIRHQMAVMEVLSGLADERGLAVVIALHDLNLAATYCHRLLVMKAGEVYG
                     YGEPEEVLTPDMIERVYGIEAVVKQDLPAPYVVPVRPGQLREGA"
     gene            complement(24901..25953)
                     /locus_tag="PLI76_01580"
     CDS             complement(24901..25953)
                     /locus_tag="PLI76_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOI60750.1"
                     /translation="MEQSVVIQEGYTRLKKTRTLFIGGLIAGLVILIGVAVTLGSADY
                     TVAESYIAILAGLFPGIFSAPDLATTIVWDLRLHRVLFAVFAGFGLAVAGSVMQGILR
                     NPLASPFTLGIASAATTGASIAIVLGAGIAGSEYLVIANAFLFALLAALAIYGMARYR
                     GIGSETMVLAGIAIMYLFSAVTSLLQYVGSAEQLRAVVFWTFGSVDKSTWPKLGIVSL
                     VLACTIPYLLYRAWDLNALAEGDEVAAGLGVPVERSMIVFMMIASLITAVIICFTGTI
                     GFIGLVAPHITRMAIGGDFRTLLPASGLVGAVLLLAADSIARSVLAPQIIPVGIVTAF
                     LGVPFFIYLFMHRRDA"
     gene            complement(25965..27167)
                     /locus_tag="PLI76_01585"
     CDS             complement(25965..27167)
                     /locus_tag="PLI76_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOI60751.1"
                     /translation="MNRRRCLPALVLLLLVAVQAAAAEGIPGDADGDRVLTRAEYVAA
                     ALAYMIGDNDLARADIQDAAQVYAFWDGRPREITDSTGKTVTLYRPLRRVVIFAGESL
                     ETLRSLGFDMEKVVAVDKYSHAKNTFFPECREMGNVGSIWSPDMERVLILRPDTVFLY
                     ATISTAACDDIQNRLEASSPGIRVLRFDCFKPATYPDEIRTIASIIGKEERGEEFAAF
                     YESTMDRIRDGAAGIPDDERTRVYFEYWFDYTTVAASAGYNEKTEIAGGTNPFATGTA
                     EYPVVDPEAILVANPEVVIKLAGQGLAVGGYAGHDIAALDAIRSDMLGRPGWSGISAV
                     KDGRVYVIHSDILGGAQHFIGTAYLAKWFYPDRFADLDPHAIHQRYLTDFQGIDFDLA
                     SEGTFVYP"
     gene            complement(27164..27682)
                     /locus_tag="PLI76_01590"
     CDS             complement(27164..27682)
                     /locus_tag="PLI76_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60752.1"
                     /translation="MITLPANRLLCIALLLMVVPVAVSGAPVTRTLSTAEPGAGEAFT
                     VSLSIEDVALGGVVETLPAGYAFAETDHPADRILVRGQKIAFAVMNDTRIAYSVRAPA
                     SGSGDITGRWEDFIGEVDGAIPASHVAVDGIETAAESGGASPVAQEAASPFAVLAAIA
                     GILIAAGRGGRR"
BASE COUNT         5252 a         8375 c         8844 g         5434 t
ORIGIN      
        1 gccggctcca gaagaggagg atcgacgcgc cgatcagggc gacgagggcg ggttcgaggc
       61 cgaggaggtc gtggatgaag aacatcccga tgacgagcgc gatgacggcg accgatttct
      121 tgaagagcga tatgtcgcgg atcgcctcct gctcgttcaa ctctgcaaac gtcttctcga
      181 tcgctccctt ctctcccggc gagacatgga gctcttttcg gtatatgagg gcgagcatcc
      241 ccatgacgac gacgatgttg acgaccatga tgggggcgag gttgaccagg aactcgttga
      301 acgtcagacc ggcggccgaa ccgatcatga tgttcggggg gtcgccgatg agcgtcgccg
      361 ctccgccgac gtttgaagcg aatatctcgg cgatgaggaa gggcagggga gtgatccgca
      421 tgacgcctgc gatgtagagg agcatcggag tgatgaggag gaccgtcgtg acattgtcga
      481 ggaacgcgct gatgacggcg gtcacgagcg agaagaggag cagtacccgc atcggactcc
      541 cttttgccag ctttgccgtt tttatggcga tatactcgaa taagccgctc ccgcgggcgg
      601 tgttgacgat gatcatcatc cccatcagga ggaagatggt gccgagatcg acgtagaccg
      661 gtatcttctc ccacgggacg atgtggagga agacgatgac cgaggcgccg agcatggcgg
      721 cgaccgcccg gtgaatccgc tcatcgatga tgagcgcgta ggtgaagagg aagatcgcga
      781 tggcgatcag ttccgggccg atcacgccct gtcaccccgg tgatggttgg gggcggaggc
      841 ggcggagcgg gtaatgcatg ccgtgagaga ttgacgaatg gtcataccgt tcctcaaaaa
      901 gaatgaattg ccgggagtta ggtttgcgcg ctcctcccca taatcatgcc ggttgcgccg
      961 tctatcgggc tctcccgcac accggggaga gccccccggt tcaggccggc gttcgacctt
     1021 tctcctcgct ccgcaaccgg aggatcttat agatctcgcc tgcaaggagg atcggcagac
     1081 cgaggaggaa gagcagtccc cagtcggcaa gggtcagtgg cgcggtgccg aggaattcct
     1141 gcaggatcgg gacgtagacc accagcgcct ggagaccgag gctcccgagg atggcaagta
     1201 tcagggtggg gtttgagaag aacccgatcc ggtggagggg gaaccggaac gaccggaagt
     1261 tcagcacgtt atacatctca aatatgatca ggccggtgaa cgccatcgtc cgggcccggt
     1321 cgatatcgac ctcgtagaag ccggagaagg cgtagagtgt caggagaccg agccatgccc
     1381 cgatgacgag gatgaccaga agagcggatc gggtgaggat ggactcgtcc gggttccgtg
     1441 gccgcctctg catgacgtcc ttctcggcgg gctcgagacc gagcgcaaga gcggtcgggc
     1501 cgtcggtgat caggttgatc cagaggatct ggacggggat gaggatcagc gggagaccca
     1561 tgaggagccc gccgacgatc gcgacgatct ctccgacgtt cgaggagagg aggtagcggg
     1621 tgaaccgggc gatgttgtcg tattctcgcc tcccctcctc gacgccggcg acgatgcttg
     1681 cgaagttgtc gtccacgagc accatgtcgc ttgactcctt cgcaacgtcc gtccccttga
     1741 tgcccatggc gataccgatg ctcgccttct tcaatgccgg cgcgtcgttg acgccgtcgc
     1801 cggtcatggc gatgacttcg cggtcgcggg agagtatatc gatcacccgg agtttgtgct
     1861 ccgccgtgac ccgggagagg actttcgtcg tccggagacg gcgcgcgagc tcgtcgtcgg
     1921 tcatcgcctc gaggtcggct cccgtcagcg ccccctcact cgagaggccc aggtcctgcg
     1981 cgacggcgga ggcggtcagg gggttgtccc ccgttatcat gatgacgtcg ataccggcgc
     2041 tccggcagag ccggatagcc tccgccgctt cagggcgtgg gggatctacg atcccggcaa
     2101 acccgaggaa gatcaggtct ttctcgaccg cctccgccgt ccggggaacg tcgcccggga
     2161 gcgggcggta tgccgccccg agtacccgga gcccccgcga tgcatacccg cagagttcct
     2221 gttggagggc gtcgcggagg tcgtcggtga gcggcacgat cgcaccgtcc cggaacagac
     2281 gggacgagag gtcgaggagg acttccggcg ctcccttcac gtaagcgacg tcgccgtcgc
     2341 cctcgcggta gatgatcgtc atccgcttcc gggttgagtt gaacgagaac tcgcggaccg
     2401 cttcgggagc gtcgtcccgg gagaggccgg ccttcgccgc cgcgacaacg agcgcccctt
     2461 cggtcggcgt gccgaagatc cgccactccc ccgcctcgtc gccggcaaat gtcgcgtggt
     2521 tgcagaggag cgctgcgcgc aggaactgcc ggagatcggg gtccgcgaga gggtcgatgg
     2581 cgctcccatc acggaggaac tcaccctccg gggcgtaacc ggtcccggtc accgccacct
     2641 cgctctccgg tgtcctcacc ctgacgaccg tcatctcgtt ccgggtgagc gtcccggtct
     2701 tgtccgtgca tatgaccgag actgcgccga gggtctccga tgcggggaga cggcggataa
     2761 ggcagtttcg ccgcatcatg ttcttgatgc cgatggcaag cgtcagcgtc acgactgccg
     2821 ggaggccctc cgggatcacc gccaccgcga gcgagacgcc gatgaggaac atctccagga
     2881 actcccgctg ctgcaggaag ccgacgccga tcgcgagcac agcaataccg acggcgatga
     2941 tcccgatgtc gcggccgagg acgtccatct gccgcgccag aggcgtcgct tcggctctga
     3001 cccgctggga gaggccggcg atcaggccga actccgtcct cataccggtg gcgaccgcga
     3061 caccccgccc ccacccgttg acgaccgtcg tacccgcaaa gaccatgttg ctccgttcgg
     3121 caagcggggt ttccggagtg agcggtcccg tcgccttatc gacgggagag gactcgccgg
     3181 tcagcggggc ctcgtccacc tgaagcgacg ttgcctcgac gagcgagagg tcggcgggca
     3241 cccggtcccc catctcgaga gcgaccagat cccccggcac gatcccggat gcatcgatct
     3301 cctgctgctt cccgtcgcgg atgacaacgg ccttctggac gagcatctgc cgcagcgcct
     3361 cgatcgcctc cccggcctgc cactcctggg agtagccgag gagagcgttc aggacgacga
     3421 tgatgaggat ggcggcggcg tcttgtggtt cgccgacgaa gaacgagacc accgcggcaa
     3481 cgatcaggat gatgatcagg atgcttgcaa actggcgcag gaagacctgg aggcgcgtct
     3541 cccgctcctc ctcgcggagg acgtttgccc cgtagcgctg gaggcgcgcc tccgcctcct
     3601 ctcgagagag gccatgcggg ccggtgccga gcgtctgttc gacctcatct gaggagaggg
     3661 tataccattc ggcaggaagg gcctcctcgc ggtccccggt cggcacgatc atactttttc
     3721 ggttttgcgg cgggccataa atagatagag atgtcgtctc ggtgactgca gcaacccggc
     3781 tctcgccggt aacattatat ccccccgggg ggttcgatag caaccgggcc gagatccggt
     3841 ccccgggagg aatgacgatg agcaggatag cagtcctgat aacggatatg ttcgaggatg
     3901 tcgagtacac agagcccgcc gcggcgtttc gtgaggccgg ccacgatctc gtccatgtcg
     3961 gcctctccgc aggagagacc gttcacggca agaaggggga ggcagaggtc accatcgacc
     4021 gggccgtctc cgacgtatcg gtggatagct tcgatgcact cttcataccg ggcggctact
     4081 cgcccgacaa actccgggcc cacgatgcgc ccgtgaagtt cgtgcaggag ttcgtccaga
     4141 gcggcaaacc ggttctcttg atctgccacg cgcctcagct cctgatcacg gcgcaggccc
     4201 tctccggccg gaggatcacc ggctggagat ccgtcgccca ggatatcaag aacgccggtg
     4261 cggagtacgt cgaccaggag gtggtggtcg acggcaacct ggtgtcgagc cggcagccgg
     4321 acgatatacc cgcgttcgtc agggcgtcgc ttgagaagct tgcagaggcc ggcgggaagg
     4381 tagaggccgc ggctgtgcgg cagcggtgag accgcgagcc gggatggcaa tcccggcata
     4441 tccttttatt tgcacagcca gatctcatgc atgggaatga agcccgcagg atcgacatgg
     4501 agcagacccg ctgtcatcgt cctgactgcg gtgctcatcg tcctcctggg ggccatcgct
     4561 cgcgtgctca ccggtatcga tgccgcgtac accgatctcc tctacctcgt gctcatcccg
     4621 accgcgtact ggtaccggcg gcatgcggta taccccggga tactggtcgc ggtgctccac
     4681 atcgtcgcta cgtacatcgt caccggacat gtgggtgcgg agacgctcgt gcgtgcgggg
     4741 atgctcatcg tcattcccta cgtcctcggg tacttcttcg aggtcgtggg ccggcgaact
     4801 gaggagaaac atttccatgc cggcgaatcc gggccggtcg catgcgaccg gagcacgaag
     4861 aggttgatag cccggttgtc gagccgggac ccggataccc gctacgaggc ggccgggtgc
     4921 ctcggcaacg cgggcgatcc cgctgcggtc ctgcccctcg ccgcccttct ccgcgatccg
     4981 gagagcggcg tacgctggaa ggcggcggaa gcactcggaa ggctgggttc gccggccgtg
     5041 gaaccgcttg cagggagcct gcagagcgag gatgtggatg tccgatggat ggccgcggtc
     5101 gctctcggcg atatcggcga cccggcggct gttccggcgc tcgcaaaggc gctcgacgac
     5161 gatgacgcct acgtcaggag cagggcggcc ctcgcgcttg cggctatcgg ggaacccgcg
     5221 cgggagatgc tcatcaccgg tctctccacc gggaacgccc gggtccgggg gagcgcggcg
     5281 ctggcgctcg ggaggcttgg gggcgagagc gccaccgggg ctctcatcga cgccctccgc
     5341 gacccggacg aggaggtccg gcaccgggcc gcggtcgctc tcggggatat cggggagcct
     5401 gccgtccccg atctcctctc ggccacccgg gccgacgagg cggacgtccg gcggggggtg
     5461 accgccgcct tcggccagat cgggaaaccc gccgtcgccc cgcttattgc agcgctcgat
     5521 gacgatgact ggcgcatccg caggggagcg gcggcggctc tcggcgatat agacgatccc
     5581 ggatctgtcg acgctctgat aagagcgctc gacgatgcgc gggaggaggt gcgagaacag
     5641 gcccggaaag cgctcggcag catcaggaag acataacatc gacgaagatg cacctgaacg
     5701 gtatgactga cgagacccgg attgatgaac tggttgccga cctccggcgg ggccctcttg
     5761 cggtgcggcg ggcggcgacc gccgaactcg gggcgagcgg ggccgtggcg gtcgaaccgc
     5821 tgattgccgc gatgcttgac ggcgacaacg acctccggtg gtatgccgcc cgggcactgg
     5881 tgcagatcgg tgaggctgcg atcgatcccc tcctcgcggc gatgcgggcc ggggagaacc
     5941 gtgatttccg acgatacgcc atggcggccc tggccgggat aggagaggcc gccgtcgaac
     6001 ctctcatcga tatcatcgag tgcgccgacc cggccctcca accgtttgcc gcgatggccc
     6061 tcatgaagat cggcgagccg gcggtcgaac cgctcctccg tctgatgcag agcccggacc
     6121 cgaagaccca ggaacgcgct gcgctcctcc tctgcaggat gggagagacg ggtgccgaac
     6181 ctctcaccgg agcgctggaa actgaccggt ccggaaaaag gtgatattgg cctcttcctg
     6241 ctgcagggtg cgggcaatcg attttttctc ggttgaggcg gcgggtgatc gcctgcaggg
     6301 tctcaacccc atccccgtac tctccgggat cgaagatctc cctgccggcg ggactttgca
     6361 cccggtaaga tgtctctaaa tgcgtgaagg gctcaatagt tcctgtatgg aggtctcacc
     6421 tgctatcgag cgatacttcg aaaacctcgc ggccgggctt gattccgcca tggcactcgc
     6481 cgctgccgcg cgggcccggg gactcgatcc gagaacggag atcgagatcc cggtagcaag
     6541 cgaccttgcc gatcgtgtcg aggcgctcct cggctatccg ggcatcgcgg cccggatccg
     6601 cgaacttgag gcggagatgt cccgtgagga ggcggccctc cggataggcg acgattttgc
     6661 agcgaggatg tttggcgaga ccacgacgga ggagatcctc gaccacgcca tccgtggcgc
     6721 gatggcgctc ctgaccgaag gggtggtggc cgccccgacg gagggtatcg cgaaggtcag
     6781 cctcgggaag aacgacgacg ggaccgatta tctcaagatc tactacgcgg ggcctatacg
     6841 gagcgccggc gggacggcgc aggcgctctc ggtactggtg ggcgactacg tccgccaggc
     6901 gctcggcatc aaccgctacg tcccccgccc cgaggaggtg gagcgctaca tcgaggagat
     6961 ccggcagtac aacaacatca tgagcctgca gtacctcccg agcgagaagg aactccggat
     7021 gatcatcgag aactgcccgg tctgcatcga cggcgagccg acggagcagc aggaggtgag
     7081 cggctaccgg aacctggagc gggtggagac gaacaccgtc cggggcggga tggcgctcgt
     7141 cgtcgcggag ggactggccc tgaaggcccc gaagatcgtc aagaatgtca agaaaatgaa
     7201 gatggcgggc tgggattggc ttgaagagat gatcggcggc ggtggtgcgg caaagagcga
     7261 tgacgacgac aagggcgccg ccgtcaaacc gaaggacaag tacctccggg accttatcgg
     7321 cggccggccg gtcttttcgt acccgatgcg caaggggggg ttccgcctgc gcctcggccg
     7381 gtcgcggaac accgggtttg cggccgccgg cttaaacccc gcgacgatgc acatcctcgg
     7441 cgatttcctc gcggtcggga cccagatgaa gatcgagcgg ccgggcaaag cggcggggat
     7501 cgtgccggtg gactctatcc aggggccgac ggtcaaactc cggagcggtg aggtccgccg
     7561 ggtggacgat gcggcggaag cgcggcggat agccggccag gtcgatgaga tcctggatga
     7621 cggcgagatc ctgatcagtt tcggggagtt catggagaac aaccacccgc tgatgccccc
     7681 gtgctactgc gaggagtggt ggcggctcga gggtggtccg cggcacccgg caagcgaact
     7741 tgaggctata gagttcgcgc tcgacggcat ccctctccac cccgactaca cctacctctg
     7801 ggacgacgtc gccccggcgg atatcgccct cctcgccgac cggatcagcg ccggcggccg
     7861 gatcgagggt ggagtgctga cgctgcccga tacaccggag gcgaaggcga tcctcgagga
     7921 gctcctggtt cctcaccgcc tctccgggga ccggatcgcg attcccgggt acctggtcct
     7981 cctcgcctgc ctcggtctga cgctgcacct cgataaaagg ccggcgtggg agaacgctcc
     8041 ccttgagaac tcgcttgacc ttgttatgca cctctcgggc ttttcgatcc gctcccgggc
     8101 gggcacccgg gtcggcggca ggatggggcg gccggggaag tcgaagcaga gggagatgaa
     8161 accgccgccc cactcgctct tccccatcgg ggacgagggc ggttctcgcc ggtcgttcca
     8221 ggcggcctgc gcctcacggc cccggtcgaa cacggacggc ggcgtgatcg aggtggaggt
     8281 gggcgagcgg cgctgcccct cctgcggcac gttcacttac aagaacctct gcgactgcgg
     8341 ggcgcacacg cggccggtcc tccggtgccc gaagtgcgac caggagatca cgaaggatat
     8401 gtgcccccgg tgcagcgttg cgacggtctg cctccagaag gtctcgatca acgtcagaat
     8461 cgagtatgcg gcggcgctcg cgaacctcgg cgtgcgcgag tcggcgatcc cgctcctgaa
     8521 gggtgtcaaa ggcctgatct cccgcgagcg gccggtggag ccgatcgaga aggggatcct
     8581 ccgggcgttg cagaaccttt atgtcttcaa ggacgggacc gtccgctacg atatgatcga
     8641 tctccccctg acccatttcc ggccggacga gatcggcgtc ccggtcgagc ggctccgcga
     8701 actcggctac acccacgaca tctacggccg ggaactcgtc gagaccgacc aggtgctgga
     8761 gctccgccac caggatatcc tggtctcgga agactgcggc gagtggcttg tccgcgtggc
     8821 gaagttcatc gacgacctcc tggtgcgggt ttacgggctc gaacccttct accgggcgga
     8881 gcaacctctc gaccttgtcg gccacctcct gatggggctt gcgccccaca cgagcgccgg
     8941 tgttcttgtc cggcttatcg ggttttcgaa ggccggtgtc ggctacggtc acccgttctt
     9001 ccacgcggcc aaacggcgga actgttttgc aggcgacacg gagatcgtcg tttccgatgg
     9061 ccggcagtgg gttacggtgc cgatccggca gttcgtggtg gagaacttcg acctctcaaa
     9121 gccggggctc gaccggacgg gaacgttcta ctccgacccc cggcagccgt tctatgcacg
     9181 aagcatcgac ccgcaggggc tggtcggcgt gaaaaagatc acgtcggtct cgatccaccg
     9241 cgccccggcg cacctcatcc ggttcgtgac ggggcggggg aaggtcctct cggtcacacc
     9301 cgaccacgcg atgctggtct gggataccga ttacctccgg aagatccggg cgctcgaagt
     9361 gaagatcggc gaccgggtcc cggtggagga gggggggctc gtcatcagcg acgtggtcgt
     9421 gagccgcgaa accgtccagg cgctcgacga ccgggtctac tgcctgacgg ttgcggagaa
     9481 ccacaccctg gttgcaaacg ggatcttctg cgggcagtgc gacggggacg aggactgcgt
     9541 aatgctcctc ctcgacggcc tgatcaactt ctcccgcgcg tatctgccgg agacccgggg
     9601 cgggacgatg gatgctccgc tcgtcctgac gaccaggata gacccggcgg aggtcgacaa
     9661 ggagagcctg aacgtcgatg tctgcgacca ctaccccctc gaggtctaca acggctgtct
     9721 tgcctacgcc cacccaaaag acctcgacaa atacgtcgac cgggtggagc ggcgtctcgg
     9781 gactcctgct caggtcgagg gattcttctt cacccacccg acatccgaca tatcggcggg
     9841 gccgcttgag tccacctaca cgaagctcgg tacgatgctt gagaaactcg atgcggagct
     9901 tgacctcgca aagaagatac gggcggtgga tgccgacgat gtggccgagc gggtcttgaa
     9961 cacccacttc atccgcgacc tccagggaaa cctcaacgcc ttctccaagc aaaaggtgcg
    10021 gtgcatgaag tgcaacgcga agtaccggcg gatgccgctt gccgggaagt gcacccgctg
    10081 cgggggaaac gtcatcccga ccgtccacga gggttcggtg aagaagtacc tggatatgtc
    10141 gcgcaacata tgcgagacct acgcgataac cgagtacacg aagcagcggg tggaggtgct
    10201 cttcatgcag atcgagtcga ccttcggcga gcccccggag cggcagctcg ggcttgcgga
    10261 cttcatgtga gggaggatgg aggcgagaga agtcctctcg gccatcaggg ccgggattcc
    10321 tatccggggc gaccggcggc ggcccgagat cgagccgttg ccggagatcc gtgcataccg
    10381 gttgacgaca cctgcaacgc gagcagatcg gacgattctc ttcttccatg caggtgggtt
    10441 tacgtccggc tcgacggccg attctctcga tctctctgcc cggctctgcg atgcggcgag
    10501 atcggaggtc gtctcggtcg actaccggct cgcgccggca caccccttcc cggcggcggt
    10561 ggaggattgt ctctcggcct accgctacct gctccacgag ggctattcgc cggagcgggt
    10621 ggtgccggcc gggctctcgg ccggggggac gcttgcgctc tcgatgcttc tctctgcccg
    10681 gggcgcgggg ctcccgatgc cggcggccgg ggttctgctc tcgccggcgg tcgacatgct
    10741 cttcctcggc gaatcggtcc tcctcaaccg ggagaccgac tggctcgcgc cggagcagtt
    10801 tgcggcgatg cgggaggatt acctcgccgg tagggatccg tgcgatccgc tggcctcacc
    10861 gctctacgcg gacctttcgg ggttgccgcc gctcctcgtg caagcggggg gcggagagat
    10921 gctgatcgat ggaattgagg ccttcgtgag caccgcccgc ctccaggggg tgccggtcgc
    10981 cttcgactgc acggaaggga tgtttcactg ctggcaggcg tttgccgggg tactcccgga
    11041 gggcgcgatg gcggtcgagc ggatcggggc gttcgtccgc cggcacgtgc cggatgaggg
    11101 atgaggtgcc ggcggggcat caagctcatt actctggagg ttcaagaagt acaggcgcga
    11161 gggtagccaa gcggtcaacg gcggtcgact caagatcgac tctcgaagga gttcgcgggt
    11221 tcgaatccct cccctcgcat ctgttttgac ggcatcgccg ccataacttc catgtcgtgg
    11281 ataaccctcc ccacaggaga gatcgcctgt cggaaactca tctccgtcat ccggttctta
    11341 tggaacaaaa tgaggatggg ggctatatcg tgatggtacc atccctttcg ggatgtgtca
    11401 gcgaagggtc tacccgggac gaagcgctgg cgcatatcga ggaggcgatc gcaggatacg
    11461 tcgaggtagc ccgggaaacc cggcaaactt cgataacatc cggcggggga tcgctcttat
    11521 cgggtgaagg acgtcataca ggtttcaggg ctgtgtgagg atgcggtcaa acgacccacg
    11581 agcggggtat attcgtcctg ttcactctca aaaatagaat aaccgaaaat tcagggcagc
    11641 tcatttgatc ttcttctcaa gcttcttgag cttcttcttt gcggcctggc tgcccgcagc
    11701 cgcctgttgc gcgagttctt ctgccttgac tttctttgct ttgttcagtt tctccatctg
    11761 tttcttgctt gttggcatac gggtaccctg ccacgcctct ggctggttgt atgtctcttc
    11821 tccatcgtat aaatatcccc gtgtcgttat accggtgaaa agacctcaaa tgagcgaaag
    11881 ccagcctatt cgactctgcc ctacatccac aacgtttggt gtggaacgac aatgtttggt
    11941 gcggaacgcg tcgcggatct tcaaggccgg gggtatcacg gaaggttccc ggcaggaaac
    12001 cgcgaagcat ctttcgtgag gcggggccca aagtcgttct ttccgggctc ttcgaggtcg
    12061 atgaccttcg caaggccatg acggatcgtc ctgttctccc cgtactcgac cggtatgccg
    12121 tcggtcaggt agcggtgaaa cgtgtggttc ttctcgatga gcgatgcgga ggagggggtg
    12181 gtgagcttgc gggcggcctc atcgacggca ctctccggga ggtgggggtt gatccggcgg
    12241 agagtgttgc ggacctgccg ggggtggaag ggttgacggt agtcggtgcg ctcctcgtcc
    12301 gcttctccgg ggacgattgc cggcccggag aggacggtgt agccaggatc ggcgaaccgg
    12361 gcaatgacgg tctcctctac ttccgattcg acgaaagagg tcattctttc aaccccttga
    12421 atatgaagat gtactccttg cccgatctct gctttgccag gtatcccagc tcttcgagat
    12481 ggaagagatc ctttcttgcc gttgcgtaag ccacgcggta tgtctcggcg atctctttga
    12541 tcgtaaccgg tttatccggt tttttgatga gggatttgat gatctctgcc tgacgaaggc
    12601 tcaacccttc actctgctga aggatggcta acgcctgttt gctctctttc tgtttcttct
    12661 cgatgtatct ctggatgctg ccgaccgcgg tttttaccgc tgtgagattg tagcggatga
    12721 aatacgtgag gtcgttttca tcgctctcgg tgtactgata tgcgcgacta tattgcccct
    12781 tcgtgtcttt tatcgctctc gataccgcca tatactcaaa cagccagtaa tccttccgga
    12841 gcatatacca gtaaaagata ctccgtgcag ttcgaccgtt tccgtcgttg aacgggtgga
    12901 tgtatccgat caggaaatgc aggataatcc ctttgatcac gggatggatg aactgcccgc
    12961 cgtcgttatt tgcaaaggca cagagtttcc cgatcagttc ctcgatcaac gcgaaatcag
    13021 gaggcgtgtg caggacgttg ccccgcatat caaagaccct gatctcgttg ttatcccgaa
    13081 accttccttc gtcgccggga tcttcgagag tatcgttggt gatggtctta tggatctcta
    13141 aaatcagctc ccgggtcaga tcttcgtctt ttatccgggt gatcctcttc atcgtggcgt
    13201 agttattcag gatcatcctc tcgtctttgg tggtgggctt cctcttctca cggagcatct
    13261 tttttgcaat cgtcctcgtg acggcggccc cctcgagctg gctcgatgca atcgcctcct
    13321 ccatcagaga attgacgaca aactggttcc gatcctgggg cagtgagtca aggccggatg
    13381 agatgcttcc cgctgctgat ttatcaagaa aatggagatt ttcgaggaaa tcatccagaa
    13441 gattgtactt taactggagg taatcgaacg gtacatattt catattcctc tctctgaagg
    13501 tcttcatcag tatccagacg tagagagggt ctaccgggag ctcgcgccat tttagctctt
    13561 cccagtgcag atattttttg ttgtatttga agaccgcatc gattacatcg ttcgtatccg
    13621 catactccca ggctttttca acatctgccg gagagtattc tggagtgcgg gggagtttca
    13681 tggtcgaatt tcagttatcg tttcactcct tattatcatt atctatcagt ttgttatatt
    13741 ttgatagata acgtacatga gacggggggt cgtccccgga cgatctcgtg cggcaatgcc
    13801 ctttctcgat ctttgccggc cgcgggcacc cggatatcac cggagatgag cgacggttgt
    13861 ttatgttgcc agctctttca caacggcctc ccggttgttc catgagaccg tttttacgcc
    13921 atagttgttc agcgcatcga gaacgtttcc aggaaccggg cgttcactgt cgttcaggat
    13981 ggcataggct tttgaatcag gggggcgggc ttcctttata tcaatccagg cgaatgcaac
    14041 tgcctgtgca gtgttcctgc tcgggttgtt gatggtcttg atgatgcgct ctggctggtt
    14101 acgggacttg gggatgataa agtcaaaaag gtggtcgaac ccgctctggc cggtgaattt
    14161 aacgttggat gcgtaccgga tatcatgaac atcaagccag gtgaccacat cttcgtaaaa
    14221 caggctcgtc gtcatgggtg ttgcaagacc gaagagatcg ttgacggcga gcatcgcctg
    14281 tatcaggttg tgtttgctgg gtgcaaaact cgcaacggat gtatacacct cgagtctatc
    14341 tttgttgagt ttcacgccaa acccgttgag cgttatcatc agcagcgctc ttctcttcgg
    14401 gctatcgagt ttgcagccgg acaactccag atcctggagg atgtatccgg catcggtgag
    14461 gatataatcg ccgtttcggc gtcgagtgta gatctggaga tagtcgttgt gccggtcaag
    14521 atagggtgtc gtgatctcca cccattcatc tacctgcctc acctgagttt tttccctgag
    14581 ccagcgggca tactcgttca ccagcccttg aatctcgttg atcattcaat gcccctctga
    14641 atatccgggg gatctactat gttgcagaac tgcataaagt cctgcaacgt ctgccatgga
    14701 tcctcgatat gtgagaacct atccgatggc acttcaactg cccacttatc accatatccc
    14761 tcccgatata tatgcaaatg gggtgaggag acctcagcgc catcgggatt cctatgaggg
    14821 gcaccgccaa agtccaaccg gaccagtgtt acgacctgcc tcgcgcggtt ctggtatgtc
    14881 cccttcagga gatcgatgct ccctctccgg atatcgaggt ggaaagattc gcgtccatcg
    14941 ctggagagga ggggcagcgt aagatatccg cccgctccgg gataacgtga cgcatcattg
    15001 ttcgccctaa ctttgggaat ggcaatcaat acatccgctg ttgcctgatc aagggagatg
    15061 tcactcatat ttgcccaacc tcacccactt ttactatttt gatccgatcg actggcatac
    15121 cgactccaat ttgtcgtcat atagcctcgt atgccattct tcgggttata ggacggatag
    15181 atcgatcgaa acacctcttc ggcctgaatg aagtcatcta tcgggagcaa atcggtatac
    15241 ttcacaagat acagcaaccc tattgcgggc gaccgtgagc acccttcgtt acagtgaaca
    15301 agaacctggt tgccggttct caggctggcg tcgatgaact ccagtgccgt atcgatgatc
    15361 ttctgtggga tgtaggctgg gtccggagca tccacgaggt tcaagataag ccgttttccc
    15421 cttcgcgcga tgaggtattc cgggtgatct tttggggcag ctcgacccct gtagcccaga
    15481 gcctgacgat ggtatggctc cttgcaggca tgaacgatgc accatcctgc ttcatgtcgg
    15541 actttaaatt cgtaatcgta ctcgttgccg acgtacaggt tcgggtgaat ctcgatcatg
    15601 ggggcaccgc gtgatggctt ttatagaggc tatgccagag gccatatata tgttacgaaa
    15661 agagtctttg ggcttggaat tccagggtag gcatgaatag gggggttttt gattcactag
    15721 atttttacct gaaaagtcac atctgggttt tggggtttgg tgcgacggtt ttaccacatc
    15781 tcaagccggg atcgtaatgg agtctccatt gtcatatggt agcgctttgt cctctctgtt
    15841 cctcccccac cggcacctgg atctccccag agatgagctt cgggggaagg gtatcgcgga
    15901 gggcggcagg gtgtgggatt caaaagggtt actctcgata catttcagta aaggcccagc
    15961 ttgcttttca aatgctccac aggctccttt tgtaggtatg gtgacataag cacctttcaa
    16021 tgtttgacaa gtgaacgtat caaaggtgga acgatgtgca ttttgtttgg atctgttcag
    16081 gagcagaatg cttcaactac taatcagata gagatcaata attcctggta acgacccctg
    16141 ggatgggtac caccatcccg gggagttcgt tgataacaga taaggccgtc acctgcgttg
    16201 cggtatatag ctgacgtcca ctcctctctt ctccagcgcc tcgatatcct tcatcgcggc
    16261 ggatcccgga gtgaggtcga ggaagttgtg gcgcagttcc acggtagcgc ctgtgccgag
    16321 ctcgctcctg acaaggggac cgatatcgcg gatctcgttt gagtcgaggt cgatcttctt
    16381 caggtttttc aggccggcga gggggccgag atcgctgact ttgttcgagt caaggtcaag
    16441 atcccgcagg ttcgtcaggt tggcaagagg ggtgaggtcg ctgacctgat tggaactgat
    16501 gtcgagttcc cgcaggtttg tcaggttggc aagaggactg atatcgctga tctggtttcc
    16561 atcgatatca agctcccgca ggtttttcag gccggccagg gggctgatat tactgatctc
    16621 gtttgattct aggtcgagat cccgcaggtt cgtcaggttg gcaagagggc cgatatcgct
    16681 gatcttgttc gagtcaaggt cgagtgccca caggctggtc aggttgacga gagggctgag
    16741 atcgctgatc tcgttcgagt caagatcgag ggtccgcagg tttttcaggc cggcaagggg
    16801 gccgaggtcg ctgatctcgt ttgaatcgag ctcaagattc cgcaggtctg tcaggttgac
    16861 aagggggctg agatcgctga tctcgttcga atcgagatcg agatcctgca ggtccgtcag
    16921 gccggcaagg ggaccgaggt cgctgatctc gtttgaatca aggtcgaggc cacgcaggtt
    16981 gaccgcatgc tcaagtcccg atagatcgct gattccttta tcctctgcat cgagagcacc
    17041 aagcccggcc atgtcatcgg tcgtaatatc tccccggggc ttatccagcg catcgcgcac
    17101 cgcagcttca aggtcggtat ccgggaacga aacagcctcc gccgccccga caggtattgc
    17161 cagaagcagc atacaggcga tcagactgca cagaaggatg cggccgaacg gcgcccgctt
    17221 ctcttcatgc gcactataac gatctgtttt cttcatacgc atcaccatcc cgggtctttc
    17281 cggcatattc ccgggcgaag ggctcggtgg ctacagctga tcgcgatatc cggaaccatg
    17341 ctgcataacc acatctcatt cgcctgcacc cccgggccac tcgcatcgag ggagcgcacc
    17401 tgtatccccg ccatccatat ataagtgcct catcgcgaac gtgagaaccg ttctccgggc
    17461 atggggagca tccgcccgat caggctgtta ttcccggcac gggatcgatc atcgccggct
    17521 ttcatgtcca tccatgagtt gccggcctat ggcggtgcat atacccggtc cttcgacaca
    17581 aacggagaag gcgccgggca cccctcgttc cgcgccgggg tcgtgcctcc ccccggccta
    17641 tcgcggttcc cctgcgccat gggtcgacat accggtggcc gagatccggc gacctgtcaa
    17701 cgcccgggcg gacttccggc agatattata cgattacggc gaagagtacc ggacgatgaa
    17761 gatcccagtc gcccggggga gagggtcgcc atagccggta agtgctgcca gtgcggcagg
    17821 tgctgcaccc acatgcggga tgtccacagg gtcatcgaag ggcgcggcaa ctacacgttc
    17881 atcgtgcaca accactatac cggggatgca caagaggtgc gggtcgaccc ggacaggata
    17941 gccctctttg aggataaaag cagtatcgag gggttgccga acgcatgctt cttcctgaga
    18001 tttgacggcg aaaaggcatg gtgcacggtc cacctgacac gccctgccct ctgccgcgag
    18061 tactgttgcc gcctgctcat ccttgatccg cagggaaggt tagcgggacg ggtgacgtac
    18121 cagagagcgc tcgttccgga taccgatgaa ttcagtcggc tctgggaaca ggtgcgaccg
    18181 gcgctggacg acctctccgg tgtggaatgg gatgatgccc tcatccgtat cctcgcgcca
    18241 gccggatacc gcgttcgccg gtagtatgcc cgggagaaga ggatcgcccc ggttctcctg
    18301 tgccgatccg gggcgagatc ggtgattacg ccgttccggc ggttccggtc ctttcgcggt
    18361 cgccgtccac taccccggca acacgcatta tggcgccgat gtccggtgtc accatgcaga
    18421 ccgggaagta gccgaagagc gagacgtagt cgatcttgag cgcccggtgc cgggccagat
    18481 caagggttat cctcttgagg tcttctgcca tcccggaggt cgcggtggcg caaagcggcg
    18541 ccgggacaag gtccgcgatg gccgggagta cctcttcgcg gatcatctcg atcctggacg
    18601 acaagagcga gtggacgagc gtacgactgt agcctccctg gagatgccgc acgacgatca
    18661 gctgcgtcag aaagataagg gcgaacatgc cggccccgag ctcgatatcg gcttcagcag
    18721 aggcgaaact gctgtacgtc accccgggga gaacgatcgc cgctatgaag atgacgccgg
    18781 caacggtcgt cacgacggca acggtgagcg cgtacctgag cccctggctc caggcctcgt
    18841 tataccgctc cctgatgcgg gactgcatac cgagcatccc cgcaaagaaa ccgcgtgaat
    18901 acggctgcac atacacgatc gcaccataaa agacgatgat tgcggcggat tgaatgacga
    18961 cgatcacggc ggagtacgac gcgaaatcac cgttcacaag ccagcccgta agagcgaaca
    19021 ccgaagagag agcgaagagc acgagaaaac ccggggcgag tggctggcag ttgatgaaga
    19081 agaggttcca gccgagttca tagagggact tcctctggtg cgtgagccgc ctgagtgcct
    19141 ccatcgcatc ggcagggatg gcggcgccct tctcaaggct cttccatgag atgatctcct
    19201 cgaggctttt tctctcgcga tctttgaggt cgataaaata cctgacataa gacctcagat
    19261 cgcctctatc ggtcgggagc aggaggagga aggggttcac catcaggaat atgaaggagg
    19321 ccatgatcca gtagagggag tactccctgg tatatgcgaa cagaagcgcc aggttgacca
    19381 gtatcgccgc tccaaggatg agcgacgtgt gctcaaggct ccatcgcatc ccggtaaggg
    19441 tcgcgagatc gccctctgcc gcagcgagga tcttctctgc ctcagcccgg gagcaggcaa
    19501 gttgctgcga gaactcatcc ggcggtatcg ccggtctctc tgcctctttc ttcctcccga
    19561 accggtcccc gtacggcagc ctcatcgatc attattttgc gggttattgt aaaaaagggg
    19621 ttcctcacca tataacccgc ctctcctcga cctcctggta aagggcatcg ccgggccggg
    19681 ctcccgcctc acccggaggt atccaggtcc accgaaccgg tatagacccc ggaaccaagc
    19741 caccaggtct cgtctatctg caccccgtac gccagtttcg actcaagcac ccggtcacgc
    19801 atggggttga tgtaggcaat ccggccgaaa ccgtccccgt atctgacagc atcgttcatc
    19861 ttccgaagga agatccccac atccccttcg gtctcgttca ggcggttgac accgatcttt
    19921 tccgggttga ccggatgggc gagggtggtg ccgttgaagt catacgcgta gatgtagagt
    19981 tcgccctgga tgaacgagcc gtttaggtcg ctgaattcgg caagcgcctt ctccttccca
    20041 ttttccctga cataagcggc cgcactctcg acgaaggcaa cgagtgtctc atttgacgtg
    20101 tagttccccc gttctacaga actgccgtct tccggcgccg atgtgtcggg agcaggcccg
    20161 ttctgcatac aaccggacga gatcaaagcc aggccgagag caagcatgac aacgagtgaa
    20221 attgcaagag accgcatact gtcagattgg gtttgccggt agatatgatt tactggcaag
    20281 gcgtagcctc tgatagccac aggagcgaca gcccgccacc ggtttcgcca gaggatgccg
    20341 ggcaccgtgc cctatcttcc ccctgcacca ctctccgctc gctccgtcag ccaggcgtag
    20401 aacttccggt gccagaacgg cacatccacc ggccgggaga gcgactgcca ggccgcccac
    20461 acgagccgtt cggagacact ctgccactcg gccgcccgcc ggaccatatc atccgccggc
    20521 tgcccgatcg cgtgcaggat ctcgtgccag atgcggagga cgatcgcctc gtcgtcggta
    20581 tctccgcgcg tgacccggac gctcgcccgc atatccatac cgcccccgag cgcccgtgcg
    20641 ggtatcgcat cgtcgaagac gtagacggtt ctttccggga ccgggaaggg gaaccccttc
    20701 cacatcggat ccttaggatc gaaatagcgg acctccggtg tccctacccg gacggtgaac
    20761 cggaagtacg gcgggatgag gtgaaggtgc ggctcaagcc tccgccgcaa ctcttcggtc
    20821 ttgaagaaga ggtcgaagtg aggcatcggg gctgcatggg tggtgggtgg tgataaattt
    20881 tcagatcgtg ggtatcgttg ccggcaggcc cgttcgtccc gatgatggtg gatgcatcat
    20941 agaggagaag cgaagatcgt gccggatata aaggcataca aaccgatacg aaacatcctg
    21001 ccccggccgt tgaacccccg gtgagaggct tttctcccat gaatacctgt atgccagacc
    21061 gccggatcca cgtgtgtgcg cggctgcgcg gcatcagacc cggatacggc agagagaaca
    21121 ctcgcgcgac gatgcggata ggccagcccc atataattgc cgaaggggaa caatgcactt
    21181 cccgcgccgg tttgccgagg attgaccgac acagcgcgaa caggaaccga ccgtgggtcg
    21241 ccgcgcgagt gttcttccgg tgccggggtt gaaccctcct gcgcggagcc ctaaacgccc
    21301 ttcaggtgcg aggcgctcac ccccggtccc cgtttcccgg aggctttacc ccgagtgccg
    21361 cttcctgctc gtctatccag gcccggatcc aggtgcggat gtcgcggatg gtagcggttc
    21421 cctgccggtc gcgcatcgcc cggatatgat cctcaagctc gtcggccatc gcaaagatca
    21481 gcgcctcgga tgcttcctgt gataactgcc ccgccgcctg caggcggatg gcgcccagat
    21541 cgaggaagtg ctttagctcg cctgtcacct ccaggaccct ggtgagattg agtctgatag
    21601 tggactccat gaagagggga ttggcgtatg cagtacataa ctccatctat ttaaacccga
    21661 gccgcatccc ggcctctcct cccagaatgg cgcacattat ggcccatttt tccctgatgg
    21721 cgcatatccc gggctcccgg gctgccggtt caggagagac tcccgggcca tccggatgca
    21781 aaaccatttt atggacgccc ggcactcagt gcaccccgct tcaggaggca tgtctccctg
    21841 aggtggaggc aatggaggtg aagaaggtgc aggaacgttc aggaacggtt ctggagcgaa
    21901 ggacgcggac gacaggggaa gaatggcaca tgtcggccga cgacatcatg aacaagaagg
    21961 tgatcaactc ccggggcgac gatatcggaa gtatatcgga tatgcgggtc tcttttcccg
    22021 agggccgggt gaagtacctg gtgctcaagc acgggggcac gctcggcatg ggggcaaagc
    22081 attttgctat cccacccgag gccgtgatct accgttcggg cgacgacaac ttcgtcgtga
    22141 acatcgacaa gcagcagctg gatgacagac cgggctttga taaggataat tggcctcagg
    22201 aagcggacac cagcctgatc cagtcatcgg gggccataac acccccgacg cgggaggagg
    22261 cggaggccat gcggcgggag gtgccaccac cggcggaagt tgtgaagaaa gagacgatcg
    22321 agacgcggcc ccggcagaag taacccggaa cagcgcctgt ttcgggcaca aaaacttttt
    22381 ttaaacccgt tcggcggcgt gatgccggga taagcaaacc acaagacaga ttagtcaaaa
    22441 aggcgagctc attatgtcgt gtctcccgtg ccagcatccg cagaaggccc ggcgccgccg
    22501 gaagtaccgg gcagcaccgt ggatctcagg gtgtggcagg tcgtcttcat agccgtaatc
    22561 gccatcgtct tcacggtagc gtacctgagc ctttatgcac agctcaacaa gaccatctgg
    22621 ggtgaggaca gtctcctggt ggtcaccccc tgggcaatcc cggccgcggt ggtcttcttc
    22681 tcgctgctcg tcggcctctc ccgcaagtac ctgcacgctc ccgacgtgat ccacgagggg
    22741 tttgcagact cgctcaaggg cggggaggga gggaaacccg atcatcgaac ctttcccggc
    22801 gccctccttt catcgctctt ctcgctcctc tccggggcaa gcatcggccc ggaagggacc
    22861 atcacccttc tcctcatgta tgtctcctcg tttgtccgga agagactcag gatcgaatcg
    22921 ccttcggccg cgctcgggtt cgatgtcgcc gcccttgcct cggcgttcaa cggcgtcatc
    22981 ggcagcgtgc tcttcaccgg ggtcttcgcc accgagtttc aggtcggcgg caataagaac
    23041 gccctccggc tcctcacctg gaacctcctc gccggcacca tcggtttcgg cttctacctg
    23101 ctcatcggct tcccatcctt tgcccggagc atcccgttcg agccgatggg cgagttcctg
    23161 ccagtttata tcctctacgc ggtcgccctc ggcatcgccg gatcgttgct cgcggtcttt
    23221 gcgggggtct ccatgcaggc ggccgagaag atcatggacc gggtatttgg cggcgcagtc
    23281 gtcacccgcg ttctcgccgc cggggtggtg gttgcggccg tcggttacgc catccccgaa
    23341 ctgctcttct ccggggaagc ccagatccac gggatcatca ccgacccggc ccggttcggg
    23401 gccgtcatgc tccttggcat ggctatcgcc aagattctcc tgctcgccct ctcgttcaag
    23461 agcgggtaca tcggcggccc gctctttccc atcatcttct catccacgct tgtagggctt
    23521 gccctgagcc tcgtcttccc gggcatcccc gtcgcgatcc tggtcctctg catcgaggct
    23581 gccgccctca cctttgctct cggtgcgccc ctgacctcca tcctcctcgt cgttgtcgtg
    23641 ggcacggccg accagagcat gatcgtgctc cttacgctct ccgccgtcac cgccatgctc
    23701 atcgccgccc gggtgaagga gcggatgggt gagcgggcgt agcggacggt ccgcccttca
    23761 ccgggctttt acacctcaaa acccatgacg tcggccgtcc atcccggtat ggcgggatgc
    23821 ggcaacgaaa ggggaagggc atgcaattca tgaacgtatt cccccgggag cccgggaaga
    23881 cggccgagat catggaaacg cgggccagag aggagatccc ggaaggcgtg accgtgatcg
    23941 acgagtgggt cgactcgcgg ggaacacaag tctttccggt gattgagacg gacgatcccg
    24001 tcgccctcat gaagctcggc ttcccctgga gtggccttgg ctacggagat gcacccggtt
    24061 atggagtcaa aagaagcgtt gactattccc gggggatgag gcggggggcg ccctacgccc
    24121 cctcccgcag ttgccccggc ctgaccggga ccacgtaggg cgccgggagg tcctgcttca
    24181 ccaccgcctc gatcccgtag acgcgctcga tcatatccgg ggtgaggacc tcctccggct
    24241 ccccgtagcc gtagacctct ccggccttca tgacgagcag gcggtggcag taggtggccg
    24301 caaggttgag gtcgtggagg gcgatgacga ccgcaagccc cctctcatcg gcaagcccgg
    24361 agaggacttc catgaccgcc atctggtgac ggatgtccag gttgctcgtc ggctcgtcga
    24421 ggaggaccac cccggtctcc tggacgagcg cccgggcgat catcacccgc tggcgctccc
    24481 cgccggagag ctcgcggatc ttcctgaggg caagcccgcc gaggtcgagg atggcgaggg
    24541 cctcgaccac cttctcctca tcggcagggc tcaccgtcca gttcaggtag ggtcggcgcc
    24601 ccatcaggac cgtctcaaag accgtcgacg ccgtctggtt cccggccgac tgcgggacgt
    24661 aggccattgc ccgtgcaata tcgcggcggt gcataccccg gaggtcccgg ccgtgaagca
    24721 gcactctgcc gctccccggg gtgagcatgc cgtcgacaca tcggatgagc gtcgtcttcc
    24781 cgcacccgtt cggtccgaca agcccgagaa cctctccggt gtcgaccggg aacgatatac
    24841 gggagaggat ctcgcggtcg cggtaggcaa acgagagatc ctcgaccgag agcagtgcca
    24901 tcaggcatcc ctccggtgca tgaagaggta gatgaagaac ggcaccccga ggaacgcggt
    24961 cacgataccg accggtatga tctgcggtgc aaggacgctc cgtgcgatgc tgtcggcggc
    25021 aaggaggagg acggcgccga caaggccgga tgcgggaagg agggtgcgga aatccccgcc
    25081 gatggccatc cgggtgatgt gcggggccac gagcccgatg aacccgatcg tcccggtgaa
    25141 acagatgatc accgcggtga tcagagaggc gatcatcatg aagacgatca tcgaccgctc
    25201 cacgggcaca ccgagcccgg ccgccacctc gtcgccctcg gcgagggcat ttaagtccca
    25261 ggcgcggtag aggaggtagg gtatggtgca ggcgaggacg aggctcacga tcccgagttt
    25321 cggccaggtg gacttgtcga ccgaaccgaa cgtccagaag accaccgccc ggagctgttc
    25381 ggcgctgccg acgtactgga ggagcgacgt caccgccgag aagaggtaca tgatggctat
    25441 cccggcaagg accatcgtct cggaaccgat cccccggtag cgggccatgc cgtagatggc
    25501 gagcgcggca agcagggcaa agaggaaggc gtttgctatg acgaggtact cgctgcccgc
    25561 gatgccggcg ccgaggacga tcgcgatcga ggcgccggtg gtggcggccg aggctatccc
    25621 gagggtgaag gggcttgcaa gcgggtttcg gaggatcccc tgcatgaccg atcccgccac
    25681 cgcgagcccg aacccggcaa agactgcaaa gagcacccgg tggaggcgga gatcccagac
    25741 gatggtcgtc gcgagatccg gggcgctgaa gatcccgggg aagaggccgg caaggatcgc
    25801 gatgtacgac tcggccacgg tgtagtcggc ggacccgagc gtgaccgcaa cgccgatgag
    25861 gatgacgagg ccggcgatga gccccccgat gaagagggtc cgggtcttct tcagccgggt
    25921 atacccctcc tggatgacga cgctctgttc catcgctgca ccggtcacgg gtagacgaac
    25981 gtcccctccg acgcgaggtc gaagtcgata ccctggaaat cggtgaggta tcgctggtgg
    26041 atggcgtgcg ggtcaaggtc ggcaaaccgg tcgggataga accacttcgc gaggtaggcg
    26101 gtcccgatga aatgctgggc gccaccgagg atgtcggagt ggatgacgta gacgcgaccg
    26161 tccttcaccg cggagatccc cgaccaaccg ggccgtccga gcatatccga tcggatcgcg
    26221 tcgagcgccg cgatgtcgtg tccggcatat ccaccgacgg ccaggccctg cccggccagt
    26281 ttgatcacga cctccgggtt ggcgacgagg atcgcctccg ggtcgacgac gggatactcg
    26341 gcggtcccgg tggcgaaggg gtttgtcccg ccggcgatct ccgtcttctc attatatccc
    26401 gcactggccg caacggtcgt gtagtcaaac cagtactcga agtagacccg ggtacgctcg
    26461 tcgtccggga tgcccgcagc gccatcccgg atccggtcca tcgtcgactc gtagaatgcc
    26521 gcaaactctt cgccgcgctc ttccttgccg atgatcgagg cgattgtccg gatctcgtca
    26581 gggtaggtgg cgggcttgaa gcagtcgaac cggaggacac ggatgccggg gctgctcgcc
    26641 tcgagccggt tctggatgtc gtcgcaggct gcggtgctga tcgtcgcgta gaggaagacg
    26701 gtgtccggcc tgaggatgag gaccctctcc atatcggggg accagatcga tccgacgttc
    26761 cccatctccc ggcattcggg gaagaaggtg ttcttcgcgt ggctgtactt atcgacggcg
    26821 acgaccttct ccatatcgaa cccaagcgag cgcagcgtct cgagggactc gccggcaaag
    26881 atcaccaccc ggcgcagggg gcggtagagc gtcaccgtct tcccggtcga gtcggtgatc
    26941 tcgcggggcc tgccgtccca gaaggcgtag acctgcgcgg catcctgtat atcggcccgg
    27001 gcgagatcgt tgtcgccgat catataggcg agggctgccg ctacgtactc ggcgcgggtg
    27061 agcacccggt cgccgtcggc atcccccggg ataccctctg ccgccgcggc ctggacggcg
    27121 acgagcagga ggagaacgag ggcagggagg cagcgccgac ggttcatcgg cgaccacccc
    27181 ggcctgccgc gatgaggatg ccggcgatcg ccgcgaggac cgcaaacggt gaagcggcct
    27241 cctgtgcgac cgggcttgct ccaccgctct ccgccgccgt ctcgatgccg tccaccgcga
    27301 cgtgcgatgc agggatggca ccgtccactt caccgatgaa gtcctcccac cggccggtga
    27361 tatccccgct cccggaagcg ggggcccgga cggagtaggc gatccgggta tcgttcatca
    27421 ccgcgaacgc gatcttctgg ccccggacga ggatccggtc ggcagggtgg tcggtctcag
    27481 cgaaggcgta gcctgcgggc agggtctcga ccaccccgcc gagggccacg tcctcgatcg
    27541 agagcgagac agtgaaggcc tcccccgccc cgggctcggc agtggagagc gtccgggtga
    27601 ccggggcccc ggatacggcg accgggacga ccatgagcag cagggcgata cagaggaggc
    27661 ggttcgccgg gagagttatc atctgtttcc tgttgtcgat tttgtaatat gaatgtttct
    27721 gattgtgcta ataagtctgc agatcggctg ttccggagta tatcgaagtg cagaccggcg
    27781 ggccccgggg cgggatcacc ccggtctcgc cggtgagagg tggaacgaga ctggagtttg
    27841 gggtaatcca ccttcagcaa ccgggagctg gaataacggc caggtatccc gggagagcgg
    27901 atgga
//
LOCUS       DAOSEE010000010        27263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_809_length_27263_cov_256.764150, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000010 DAOSEE010000000
VERSION     DAOSEE010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 27263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27263
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_809_length_27263_cov_256.764150"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..445)
                     /locus_tag="PLI76_01595"
     CDS             complement(53..445)
                     /locus_tag="PLI76_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60753.1"
                     /translation="MLERAGYIQRGRCAAVDGEGRSSRIVEAVLNGRQFGVRVWELRQ
                     ALDGRRPARVEVLRRDWGPVLGGTVGRAEVLGAKTAITLTLATGERYTVPIVALRALL
                     ARAAAFAPVAAVRSRGDSSTMGQALTPG"
     gene            complement(518..1915)
                     /locus_tag="PLI76_01600"
     CDS             complement(518..1915)
                     /locus_tag="PLI76_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60754.1"
                     /translation="MPVPDLFSAMMKEEWRIHATIFGSAGFAFFPVVVAIFAFAGSLA
                     LPLFSDTLPSTVMAMLALAFFLLAGVMVGSFGTMGREFMNRRFGQASLVAYASRSLPL
                     SERRIFATFIVKDTVYYILLYVLPAAAGFTAAAPFIALDPIWGLRAFVSLALAFLIGL
                     SAVCLLSTLYARSIGLLAGTVVVLAGAGLAALGMNAVFPPYAFLIDPAPGSLALSLIL
                     ILVPAAASVLLVTVDYPDRTKRFTNRFEPLAERLCPLGSAHFIAKDALDLSRSEGGAG
                     KVIFSFILPLGLVWVCLRILIRFIPGIDPLVVFAILLGVISATIYNWLTEFDSFTSYT
                     FLPVEVSAVIDGKLKSYAIANLLPVAVLVLAAASAGGAGSLLPALAAFLSVSVYTLAV
                     TVYLCGLSPNIMLYHAWVFLRYVLMISPALLLLIFASLLDPAYAFGSLFLVPAAGLLI
                     ARARSKWQAWDMPGY"
     gene            complement(1902..2618)
                     /locus_tag="PLI76_01605"
     CDS             complement(1902..2618)
                     /locus_tag="PLI76_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI60755.1"
                     /translation="MIEATELTKNYGNVTAVNGISFSVMEGELFGLLGPNGSGKTTMI
                     RMLTGQVRPTAGSARVMGLDPAEDPIGVRQLIGIVPEQETPPSFLTAEEYLHFVARIR
                     NLDRIDERCDRWFDLLGFQEKRDVLCKDLSRGTRQKLMVAQAFLHEPRLVLIDEPLIN
                     LDPIVQRTVKDFLQEYVRDGGTVLISTHILEIAEEICDRVGVLHDGKLLYCGQTEGLA
                     GPGRSLERSFLDLVRGDAGA"
     gene            complement(2932..3054)
                     /locus_tag="PLI76_01610"
     CDS             complement(2932..3054)
                     /locus_tag="PLI76_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60756.1"
                     /translation="MRVERMECKADPRKNATQKDLSTPGSVTILRMKEKTLKTI"
     gene            3201..5408
                     /gene="glgP"
                     /locus_tag="PLI76_01615"
     CDS             3201..5408
                     /gene="glgP"
                     /locus_tag="PLI76_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOI60757.1"
                     /translation="MKTEIPYDRFAHVPERIFGLVDLAYNLWWSWHSSASILFKLLNR
                     AEWKMSRHNPVRMLLETPPRFFERAATNPEYLRRYDIIMFRFREYMEPRTSWFAEHYP
                     GRVPLTIAYLSSEFGLHHSLPFYAGGLGILAGDHIKASSDLGVPMVAVGFMYAEGYLH
                     QHMEADGWQKNVAEILDRDAAPVLRVLDDNGRHLVVRVPLIDPPIYVAVWKVQVGRVP
                     LYLLDTHIDENLPENQSISHRLYLKELECRLRQEIVLGIGGRKVLHALGVDYSAMHLN
                     EGHSAFAILERLRERLVAGMPPDEALEKVRGTSIFTTHTPVPAAHDIFPEDLMARYFR
                     SYCPSLGMTWDEFMALGKDPHNPGARFNMTAFALRMSLFHNGVSRRHGEVAREMWQPL
                     WPDLPLEEVPIDAITNGVHLPSWLNHRIEALFDRYMDVIYPNWREDYDNPIIWEVVDE
                     IPDNELWGEHQWLKMKLFARLRERKRLKWAGHREEPANLAAEGLMLDTSALTIGFARR
                     FAEYKRADLIFEDLDRLDSIVNNRWRPVQFIFSGKAHPADEKGKRILQRIYQYSQDPR
                     FGGRISFIEDYGEQLARYMVQGVDVWMNTPVPLMEASGTSGMKAGMNGVLNLSVLDGW
                     WVEGYNGRNGWGFEGHTPYAGNNRADADTIYDLIENEVAPLYYSRTMDNVPHKWVQMM
                     KESIKSIAPEYCSFRMLKDYIRSYYPSVCMYAAEPERQLAGIEGVCLPTNPFR"
     gene            5549..6418
                     /locus_tag="PLI76_01620"
     CDS             5549..6418
                     /locus_tag="PLI76_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOI60758.1"
                     /translation="MTGSRSARKVLIAVAVAAMLGAALIVAPPATPATVLLLFTIGIV
                     AGIFGGLIGVGGSTIMLPIMYFYLGFPEPIAVGTGLFVVIFTSLSGAAGHLVRGNLDR
                     RVAGWIAAGGLVGVIFGSWLFSFLVGYTRLLSLVLGLVFLVPSLGMIRDGLRPATRPE
                     PGCIGGTPPGHLLFGTGVGVLTGITGLGGGYALVPGLLYIFGAPVAVTMGTSLASMIP
                     MAILGGGIKLVEGYVAVGAGLTLAAGSIAGAQAGAATIHRFRPETLKLIFGFYFLYAA
                     IRFITGFFGVGVP"
     gene            6481..7440
                     /locus_tag="PLI76_01625"
     CDS             6481..7440
                     /locus_tag="PLI76_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60759.1"
                     /translation="MEWKDLLPVLAGGLVILAVALVVKPALVGGPATPPGSLPPAYVP
                     PAPLPAATPYPEDGAVYTRDFRWTAIDGGVLTTEVRVPEALFRDHRETPRIEDRLAYG
                     RYVLADADRPILEDLVRQIAPPVASPDAEYYRLMNVVFFVQQIPYEYDNSTNSYIEGV
                     LPRHALHIGDGIEYQKYPAEMLVDGKGDCEDAAILMAGLIDALGYDAVLLRYRGDTKL
                     GIAGHMALGIQMNEFNPYYAKYTPRYFEHGGKHYYYIEGTDYLKFSAGNVTSWGKPFP
                     IGDVDGTGIEAVRSRIPEIIPLRYTPAPAEYRIRSVGLPAGGG"
     gene            7446..8042
                     /locus_tag="PLI76_01630"
     CDS             7446..8042
                     /locus_tag="PLI76_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60760.1"
                     /translation="MDITVPKLPTLPKLPAIATNRRQALIAVGAVIAVVAAAFIGWTL
                     FTDWTESRQVAVFGSHVAASEADLARVSAKISSHMSSRPEAPSIAESDVVMREFAAIA
                     TYGQAVTTYHRRIIGADEVPEAYIGAQSAYIRALDCLNRAFSLWASAAAAYDAGAYTS
                     AKDALVKADQAWKEYVAATGDYNRELRRAEEGEEAPPV"
     gene            complement(8039..8692)
                     /locus_tag="PLI76_01635"
     CDS             complement(8039..8692)
                     /locus_tag="PLI76_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HOI60761.1"
                     /translation="MQGRHILLLILLTATILACGCTADGERSETQPASLTPAPSSAQP
                     QMSGDGMSAPTPAAPVRGNATQEVRKVEFVDPAVYRIPTPTPTIAIGKQPDDIKVSEK
                     MVDYARATINYPPGVLATETYHIPFPYWAFNVSITPMGEHPWLTIDVHEKDDPNRAVI
                     ETIRYSRNSFPYEGGMPREKKERFMILEGFRDYYFIIRSESVKSFELTILIPEKYLV"
     gene            complement(8826..9326)
                     /locus_tag="PLI76_01640"
     CDS             complement(8826..9326)
                     /locus_tag="PLI76_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOI60762.1"
                     /translation="MQKTIERREVQEETTAEVRELVHVMLNADTYTSLQAISTLGAIG
                     APAVGPLVETLLAAGSDARWTVAMALARTGEPAVEPLIGVVLVADDELKNPAIWALAE
                     IGDPRAVNPLVGTLKSGQSECCRALTAAALLKLGDPAGIAEVWKVFDREGEPFRGLAM
                     EALEGT"
     gene            complement(9455..11371)
                     /locus_tag="PLI76_01645"
     CDS             complement(9455..11371)
                     /locus_tag="PLI76_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOI60763.1"
                     /translation="MQSEQDIPGRILRSLKFRPKGMTITEIAKTLKSNRNSVSKHLEV
                     MQAAGQVEARLVGNAKVYSVAQRVPLSAFLCFTKNLILVLDADLTIVQANDQCLRRFG
                     RTKEELVGRNLREAALPVVSSLKSLALVENLEREQVIADIPHRRNDGSESFYQMQAIP
                     TTFEGGERGCTLILEDITERKRYIRNTEFLARTAIDLVDLQPVEDIYQYIADRLAELV
                     PGAYAYILSYSEAEHQFAIRATAGEGFREGLTELLGRNPVGQILPVTIVFEAPYHQTP
                     LSMRGLQEFVLRPEPSAWPFYDICFRAIPEEICEAILERFNIVKLCLTGLVWRDRLYG
                     ITGIFLPPGREVENKEIIESFIRQVSIAIARRETEERLRLSGVRFRSMLDASPFGIAL
                     IDHERRFCYCNRRFSEIFGYETGRAVTATEWVRQIFADDSDWQEAVAAWRSDLERSAP
                     GQIRPRTFVVRSRDGAKKTILSRAVTLPDGTEYVTCEDITEEARAYRLLVADIADLRR
                     QEQELLIKDRAIASTRRAVALLALDGVVTYANAAYLALWGYPAAVEVQGSPFSGFWEH
                     PDKIRMIIRAVIGGGAWHGELVGLRKDGTRFTVDLLGTPIVAESGRVIGMMAAATRLP
                     KEQGKPEKTRNAPT"
     gene            11579..12316
                     /locus_tag="PLI76_01650"
     CDS             11579..12316
                     /locus_tag="PLI76_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOI60764.1"
                     /translation="MEEHELVSITQGGFAIMNPTTPEKVLAAGRAAGLRKGSRVVEIG
                     CGNGTILALWGREYGASGLGIESREDACRRAEETFRAAGPGGRLRVRCMDAREYVSDE
                     PFDCAASIGASHIHGGFAATLDALLDLVHDEGSVVIGDRFWRSDRIPPEFARAWPDVP
                     TGYEVLGTAREAGLDLAAVIRADGADWDRYESGIWQSVLSWLGAHPDHPDRDSMIDYL
                     HRLQDEYFGYGREYMDWALFVMVPGFW"
     gene            complement(12341..13996)
                     /locus_tag="PLI76_01655"
     CDS             complement(12341..13996)
                     /locus_tag="PLI76_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOI60765.1"
                     /translation="MRTPACIAILLVAVVVLGAGCVGESQPPPEEAEKSVLLEAVARI
                     NGELESINASIGESARVLGETGLRSAAGKEAVQRAMLNHPYTESTLVATKEGIVTMAV
                     PESYAGTVGSDISSHAETERSVQEQVPLVSEVFPLAEGYAGVAQSYPIFGAGGEYIGF
                     VSVAYRSDALIGRAIVPLINGTPYDIWVTQTDGLVVYDTTPEEIGRNLFADPVYQAPG
                     LQEAFSRIVAEPSGSLEYSFWERNWERNVTKKAIWDTAGISGTEWRVVATRSADAGEV
                     PATTGERPPVADTAGDMKAFVGEAAAYAREHGRTDALAAFNNPAGEFVRDELYIFAYD
                     MNGTVLALPFQQALIGADRSGVHDVSGVAYIAGMKRLAAEGGGSINYIYPNPARGYAE
                     DLKLGYVLPVDETWFVGSGVYIPGVAATFNTSVRETLVQRVKAARDYAQKQGRAAACA
                     AFNDVSGDFADGEAYIFAYTMNGTTLALPYQPEYIGENRFDLEDRYGVRIILLESAAA
                     ESGGGFVYITYYNPDTGVDGLKLCYVAPVDGEWFVGSGVYAGA"
     gene            complement(14403..15656)
                     /locus_tag="PLI76_01660"
     CDS             complement(14403..15656)
                     /locus_tag="PLI76_01660"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HOI60766.1"
                     /translation="MKILIVDPGCSGVDGSILLASLIDLTGEPRALDPLAGAIRDLGI
                     CREFRYEAERVGAGEEVAATLLSITAREEGAMAGDDFQEACMAVADDVGLSPGARDRV
                     SSVLGDLTAVSFRPAETIFSVVGTMFLLDRAEFFEGTIVAMPPALGATITRTDCGDIP
                     GPAPTTLAILARHRFPFASVPAEADLTTPVGAALLAAITERSISLYPAMTPARVGYGI
                     RPSGEPTGLLRVMEGESLPPGEDRVVMLETNLDDTSGEVIGYTIERLFAEGAADVFVT
                     PALGKKNRPVSVVSVMAAAADENRLIRILMEETGTLGVRVREFRKVVADRRKETVPVS
                     VGGREYPIRVKTASANGRPVAEKPEYDDLAAIARNRGIPLRLVGDEVRLCLSERRRER
                     TAREGGAGGGCPPDLPSAANTARDG"
     gene            complement(15669..17510)
                     /locus_tag="PLI76_01665"
     CDS             complement(15669..17510)
                     /locus_tag="PLI76_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60767.1"
                     /translation="MEIERERVLLAAAHAVTYEPDYMTVDKLEAATRGHGALVIPAFA
                     AANSLAGDIFCSIGAPEMRLMDMAVVDASAPSLPLDSVIAKAVGEAKRGGAEPANAAL
                     IVATLAYFSGSCARAGVPLGNRKLGAIARMHAGGDRTSAIALTTNKFTHRVPAFPAYL
                     AIYEALMGKRLTRLDGAVLPPFVAGGGVMGHAALGEDYNIPELARNAAMVGTRALMRS
                     MEGAGTTPYPLWPALIAAAVTMELVHPDALLGEEFGKFGEVDSSYLAGKGAVEAAGLP
                     KKIHMRGTGEEYETARLVGDFGLILKDVGGPSVIGSMVTNELFAGFQESAAIGAGFSG
                     GPVNVPLGHLAGDILPALRLLIGNGGDIERTAAAIRDYKMQSFIDPETALCATNTITR
                     KAEVINRGPVTDACIRASEPTRNRAIYRRAVKVYEMLKEGRTLEEAAGALDEEKKALI
                     ERRGSAILSGFVGKEITFRFTEIAPHARRTDPLTARFWGFDAKVSYDVRIDGESYHIE
                     NLTARVIPEYALEGKGRDDPGMGTAIFAGAVLTQELQYGGNTILNITVPAAVAAALGT
                     SPAEAATGAAKGARLTNAIPGGRAVAERVATIAQDIVANLGKPDEVA"
     gene            complement(17598..18089)
                     /locus_tag="PLI76_01670"
     CDS             complement(17598..18089)
                     /locus_tag="PLI76_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF367 family protein"
                     /protein_id="HOI60768.1"
                     /translation="MIPLYAYRDNTCDPRKCTVKKLARRGLATIVTNIAKIPRSTLLL
                     DPTAERAVSPADRHLPSITALDCSWEVLDTGAVASWRNRRALPFLVAANPVNFGRPFR
                     LTSVEAMAATLSILGEEEQARTILAPFGWGLRFLEVNAEPLEDYAQAKDSAEVVAIQA
                     LYM"
     gene            18125..18964
                     /locus_tag="PLI76_01675"
     CDS             18125..18964
                     /locus_tag="PLI76_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HOI60769.1"
                     /translation="MYVLVVPCIENPACRARGITTDEDLRHFSRAVERCRHFSIDMVP
                     LPCPETIYIGADRPPGSYLDRLATPAFAALLDRLEAEVRAVIRERGEAPLALVGVDSS
                     PACGVTATYYSSVKQPGRGAFLARFPDIPAIDVKEFARYRIYLAGPLFSEAERAYNLI
                     LHDLLAVHLFDVYLPQEAGDTDPARCGEEHGAIFAQNLLALRDADVVVAVIDGADADS
                     GTSWEMGYAYALKKKIVALRTDFRVVGHHERVNLMLEESAAVVTKKEDLPRALGSLLP
                     ASP"
     gene            complement(18956..19366)
                     /locus_tag="PLI76_01680"
     CDS             complement(18956..19366)
                     /locus_tag="PLI76_01680"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI60770.1"
                     /translation="MAELTVRAELGALTAIADFLAGTLAGSADDMVFSVQLAVDEACS
                     NTILYGYPNREAGAITIACTVDDDVVRVTITDDGVAFDPLTAPPPLLDIPAEERPIGG
                     LGIHFIRTVMDNVAYARRDDRNVLSMEKRRPASG"
     gene            complement(19366..20733)
                     /locus_tag="PLI76_01685"
     CDS             complement(19366..20733)
                     /locus_tag="PLI76_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOI60771.1"
                     /translation="MAFSEFGDLFFVLFQMICVILVAAYLITRTASFTRVLDGLVTWK
                     SQAILILLFGALSIYGTESGITILGATANVRDLGPMVGGLACGPVVGLGAGLIGAAYR
                     FSLAGFTAVPCSAATILAGLFGGLIFLLSGRKFVGIHGAVLFAIGMEAFHMGITLLLC
                     RPFDQALTVVREVALPMIIANAIGVFIFALIIGNLITERQTKAERDAYLSELERKKAE
                     LRIAHDIQMSFLPERLPDVPGLDIAAFSLPAKEVGGDFYDAIPLSGQRIAFVIADVAG
                     KGVPAALFMALSRTVLRANALIPRSARDAVREANALIAADAKSGMFVTLFYAVVDPVK
                     KTLTYVNAGHNPPLLFRSGSERVLELKGTGIILGVMPDADYGEETLALEKNDLVLLYT
                     DGVTEAIDSHEEQFGEQRLIETVMGCRDLPSREIVDRIRRAVADFSGDEPQFDDQTLM
                     VLRVV"
     gene            complement(20739..21095)
                     /locus_tag="PLI76_01690"
     CDS             complement(20739..21095)
                     /locus_tag="PLI76_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOI60772.1"
                     /translation="MSERVDILERRAGAVDIIAVKGRLDAGSSEKAEERINRVLDAGG
                     RYLLINLCDLDYISSSGLRVLLAALKRLKKDGGALRIACAQPQILEVFTMAGFHRIFS
                     LSPDEATALAGFPAGP"
     gene            21599..23275
                     /locus_tag="PLI76_01695"
     CDS             21599..23275
                     /locus_tag="PLI76_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOI60773.1"
                     /translation="MDPTTGALSLLLSNLKEHPRGMSVSDLAAAVGINRNTVSRYLDV
                     LLVSGQVEMETYGKAKVFYLSQRVPIAAMLNFSSDLVLSLDRELRIVQANDAVCAFAG
                     RERDDIIGNGIEASPLAAFDHPLIRGRITDALNGREVTEELRFLRHDRELFFRAKFLP
                     TVFNDGAPGVTIILEDITEERRAEEALRESELLFRSLVENISDLILNVDETFRFTYVS
                     PKSREILGYAPEEMLHKTPCDFMAPENAERARAQFSALLADPEPRILFEWTMLHRDES
                     DVILDVSGTPVYDVIGDFTGYRVVCRDVTERARAAKRVAQWKSFLYSVVNNVPGMVFV
                     REVEGNTFVFANRAAEIFLGMTQDEMAGRQAADIFPPEMAVFFADGDRELVERAAALE
                     KEIRISGGRTLSMKKIPIFNSRGVLRYMLGIAEDITDRSAAEDLLVAERDRAQGYLDA
                     AGVMIAVIGADGTIDLINRRGSELLGYAGDDLAGKNWFKTVVPERLRDPLARNFARLV
                     AGRIEPPAFEESPVVTRDGRERLIRWHNALLRDADGNVVAMVSSGEEPAG"
     gene            23375..23953
                     /locus_tag="PLI76_01700"
     CDS             23375..23953
                     /locus_tag="PLI76_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOI60774.1"
                     /translation="MVTTLVDIISLVLIAVVVLIVIGIVAVMIGIYNRFFSLKNSADA
                     TVGQVKVAMKKRLDMIEQLLGAVKSYAAFEKDTLTGVTAMRARIGSAGPGDLNELERE
                     SRSVLGRLIAVAENYPNLKTSQTVQDLMGAVKGIEDEIARHRYTYNNIAQQYNTMTDT
                     IPSNLVASVMGFKKLEYLEFGEDIERVPTIAF"
     gene            23960..25786
                     /locus_tag="PLI76_01705"
     CDS             23960..25786
                     /locus_tag="PLI76_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOI60775.1"
                     /translation="MRVTEQQQILILVAITLLIGVLAVAGAVALPGLLQGDLDVGHYD
                     AVFYENGTLIERYTYDVRAAGEYRMLFRYWDAPLTFAAIDQPHVEFLGMTAPPGVIGY
                     ARDSGGEVRTAAGSATPSDLATIRNLALDSEVGIFNPGYFAPGTYTVEYRYLVRPPVE
                     YDSQWAHLNLKLVDEHVPFRNLRITLPFAGDIEEVYTHPPTLDIERTADAVVITGSAS
                     QNDALNVELILDPAFTEKVGGFGEFVPDVRQKAADANRWPPLLYGAANILYGLSILLV
                     LATPFILLGVYLRYGREKPFTVPEYLSFTPNTALKPWQVNLLFKGDALEFDDNGFYAT
                     VLDLHRQKKIVVTEKSGGNGVTVRIVSGESSDPYEQRVLNFLANIADDHVVDTADLET
                     FAETARRSPGYQHRILQYQQSLAALTRNVDTTLARQYVSDGRGLILPLIFLGAIPCGL
                     AILTIIFAPGAAYLLIPAGILSFIVAVQVGIAALFPSTLFGVWKGDRYREKLEWDAFA
                     HFLSDLALIRQYSPADLSMWGEWLVYGTALGLGDKVEQAMKNLNISLPDVGMPLYSNM
                     PVIFAPIVFYSPPSSGGGHGGFGGGGSFGGGGGFGGGGVGGR"
     gene            25887..27065
                     /locus_tag="PLI76_01710"
     CDS             25887..27065
                     /locus_tag="PLI76_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HOI60776.1"
                     /translation="MRGSYLNYSAVVLALLSVAVACGCMAGGEKDGTRDRISGNGTIT
                     YLDLEGGFYGIVADDGSRYLPADLPADFRQDGLRVAFVVDRAEETATIQQWGTPVDIV
                     SMEKGDARRTVTANGTVTYIDLEGGFYGIIAEDGMNCLPANLPEEYRIDGLSVRFSAD
                     LEEDVVGFPMWGIPVNIRTIEPSGGVRLVAGNGTITYVDLEGGFYGIVADDGEQYFPL
                     GLSEAWLVDGMDVAFVARAREDVAAIGQWGAPVDVIAIDKAGSATFVAENGTVTYIDL
                     EGGFYGIIADGGRHYLPLGLEERYRVDGMRIAFAGKIARDIVTIQQWGTPVKILAVPW
                     ACSSCGGSAGIANPAAAWCLAQGHAYEIRKNPDGSEYGVCIFANGTVIDEWDYYRQSH
                     "
BASE COUNT         4807 a         8652 c         8692 g         5112 t
ORIGIN      
        1 tgcggcgcac tgggacgagt acgcaaagag gcgggtgacg gcatgatcgt ggctacccgg
       61 gtgtcagggc ctgccccatg gtgctcgaat cgccccggga gcgaaccgcc gcgacggggg
      121 caaacgcggc ggcacgtgca agaagtgccc ggagcgccac tatcggcacc gtgtagcgct
      181 ccccggtggc gagcgtgagc gtgatcgccg ttttggcccc caagacctct gcgcggccga
      241 ccgtgccgcc gaggaccggc ccccagtccc ggcgaagcac ctccacccgg gcagggcgcc
      301 ggccatcgag cgcctgccgg agttcccata cccggacccc gaattgccga ccgttgagaa
      361 cggcctcgac gatccgggag gaccgcccct ccccgtcgac cgcggcgcac ctccccctct
      421 ggatgtaacc tgctctctcc agcatatgct atctgtctcc ctcacgactc taaaagccgc
      481 gccgtgcggt gtgaaagtgt aaggtgggga tggggtctca ataccccggc atatcccacg
      541 cctgccactt cgaccgggcg cgggcgatga ggagccctgc cgccggaacg aggaagaggc
      601 tcccgaaagc gtacgcggga tcgaggagcg aggcaaatat caggaggagg agggccgggc
      661 ttatcatgag gacgtagcgg aggaagaccc aggcgtgata gagcatgatg ttcggggaga
      721 gcccgcagag gtagaccgtc accgcgaggg tgtagacgga gaccgagagg aaggcggcga
      781 gagccgggag gagagacccc gcaccgccgg cactcgccgc cgcgagcacg aggacagcga
      841 ccggaaggag gtttgcgatg gcgtagctct tcagttttcc atcgatcacc gcggatacct
      901 ccaccggcag gaaggtgtag gaggtgaagg agtcgaactc cgtcaaccag ttatagatgg
      961 tcgccgatat cacgccgagc aggatcgcaa agacgacgag cgggtcgatc cccggtatga
     1021 acctgatcag gatccggagg cagacccaga cgaggccgag agggaggatg aacgagaaga
     1081 tcaccttccc ggccccgccc tcgctccggg agaggtcgag ggcatctttt gcgatgaagt
     1141 gcgcgctccc gagcgggcaa agcctctccg cgagaggttc aaacctgttc gtaaacctct
     1201 tcgtccggtc cgggtagtcg acggtgacga gaaggaccga tgcggcggcc gggaccagga
     1261 tgaggatgag cgagagcgcg agcgatcctg gagccggatc gatgaggaag gcatacgggg
     1321 gaaagacggc gttcattccg agagctgcaa gcccggcacc cgcgaggacc acgaccgttc
     1381 cggcaaggag ccctatggag cgggcgtaga gggtcgagag gaggcagacc gcggagagac
     1441 cgatgaggaa ggcgagcgca agtgagacaa acgcgcgaag gccccagatc gggtcgagag
     1501 cgatgaacgg ggccgccgcc gtgaacccgg ctgcagcggg gagaacgtag agcaggatgt
     1561 agtagacggt atccttgacg atgaacgtcg cgaatatccg ccgttccgag agcgggaggc
     1621 tccgggaggc gtaggcgacg aggcttgcct gcccgaaccg gcggttcatg aactcccgcc
     1681 ccatcgtccc gaacgaacct accatcacgc ccgcaagcag gaagaaggcg agcgcgagca
     1741 tggccatcac ggtagagggg agcgtatcgg agaagagggg gagggcgagc gagcctgcga
     1801 aggcaaagat ggcgacaacc accggaaaga acgcaaagcc ggcgctcccg aagatcgtcg
     1861 cgtggatccg ccactcctcc ttcatcatcg cagagaagag gtcaggcacc ggcatcgcct
     1921 cgcaccaggt cgaggaagga acgctcaagg ctgcggccgg gcccggcgag cccctctgtt
     1981 tggccgcagt agaggagctt cccgtcgtgg aggaccccga cccggtcaca gatctcctcg
     2041 gcgatctcga ggatatgggt ggatatgagg acggtcccgc cgtcacggac atactcctgc
     2101 aggaaatcct tgaccgtccg ctgcacgatg gggtctaagt tgatcagcgg ttcgtcgatg
     2161 aggacgagtc ggggttcgtg gaggaaggcc tgcgcaacca tcagtttctg ccgggtgccc
     2221 cgcgagaggt ccttgcagag gacgtcgcgc ttctcttgaa accccagcag gtcgaaccag
     2281 cggtcgcacc gctcatcgat gcggtcgagg tttcggatgc gggccacgaa gtggaggtac
     2341 tcttcggcgg tcaggaaact cggcggcgtc tcctgctcgg gaacgatacc gatgagttgc
     2401 cggaccccga tggggtcttc tgccggatcg agccccatca cccgtgccga tcctgcggtc
     2461 ggccggacct gcccggtcag catccggatc atcgtcgtct tccccgaccc gttcggcccg
     2521 aggaggccga agagttcccc ttccataaca gagaacgata tcccgttcac ggcagtgacg
     2581 ttgccgtagt tcttcgtgag ttctgttgcc tcgatcatct gtcgcacgcg agtcaccctc
     2641 caccagtctc cagggaaaca tcagcagcag gcccctataa tactttttca tccgcatcgg
     2701 gagggctgat gcaggatctt caaaaacagg ccacatatcc ggccacctcc ctcctctcac
     2761 ccctgtgccg gcaccggccc gcgtctcccg acggcagcct gccctgctgg agcgcacggc
     2821 gggtataagc accctcgcag atcgctggag cccatggatg aacagggacg acatgcccgc
     2881 gcgaggaggc gggtcgggga gatccgggga tttttacatg catcttgcag tctatatcgt
     2941 cttcaacgtc ttctccttca tcctcagtat ggtgaccgac cccggggtgc tcaggtcttt
     3001 ctgggtggcg ttcttccggg gatcggcctt gcactccatg cgctcgacac gcatgccggg
     3061 ggcaggttct ccggcagaga gtgggaaggg cgaaagatcc gcgatatcgt ggagcgcgaa
     3121 gagcgacggt gacccggttc ccgcccggcc ggccgggtga tggaagcact tatcaggtgc
     3181 gacaggcaat gcccgttgcg atgaagacgg agatacccta tgaccggttc gcccacgtcc
     3241 cggagcggat cttcgggctg gtggaccttg catacaacct ctggtggagc tggcactcct
     3301 ccgcaagcat cctcttcaaa ctcctcaacc gggcggagtg gaagatgagc cgtcacaacc
     3361 ccgtcaggat gctgcttgag accccgccgc ggttcttcga gcgggcggca acgaacccgg
     3421 agtacctccg ccgctacgat atcatcatgt tccggttcag ggagtacatg gagccgagga
     3481 cgagctggtt tgcggagcac tatcccgggc gggtgccgct gacgatcgct tacctctcca
     3541 gcgagttcgg gctgcaccac tcgctcccgt tctatgcggg ggggcttggc atcctcgcag
     3601 gcgaccacat caaggcatcg agcgacctcg gtgtgccgat ggtcgccgtc gggttcatgt
     3661 acgccgaggg atacctccac cagcatatgg aggcggacgg ctggcagaag aacgtcgcgg
     3721 agatcctgga ccgcgatgcc gcgccggtcc tccgggtgct cgatgataac ggccggcacc
     3781 ttgttgtccg ggttcccctg atcgaccccc cgatctacgt cgcggtctgg aaggtgcagg
     3841 tggggcgcgt ccctctctac ctcctcgaca cccacatcga cgagaacctt cccgagaacc
     3901 agagcatatc ccatcgcctc tacctaaaag agctcgagtg ccggctccgg caggagatcg
     3961 tgctcggtat cggcgggagg aaggtgctcc acgccctcgg cgtggactac tcggcgatgc
     4021 acttgaacga gggccactcg gcgtttgcca tcctcgaacg gctccgggag cgcctcgttg
     4081 ccgggatgcc cccggatgag gcgcttgaga aggtccgggg cacgtcgatc ttcaccaccc
     4141 atacccctgt cccggctgcc cacgacatct tccctgaaga cctgatggcg aggtacttcc
     4201 gcagttactg cccgtcgctc ggtatgacat gggacgagtt catggcgctc gggaaggacc
     4261 cgcacaatcc gggcgccagg ttcaacatga ccgcgtttgc cctccgtatg agtctctttc
     4321 acaacggcgt ctcgcggagg cacggcgagg tcgcccggga gatgtggcag ccactctggc
     4381 ccgacctgcc cctggaggag gtcccgatcg acgccatcac aaacggcgtc cacctgccct
     4441 cgtggttgaa ccaccgtatc gaggcgctct ttgaccgtta catggacgtg atctacccga
     4501 actggcgaga ggattacgat aacccgatca tatgggaggt ggtcgacgag ataccggaca
     4561 acgaactctg gggcgaacac cagtggctga agatgaagct cttcgcccgg ctccgggaga
     4621 ggaaacggct caaatgggcg ggacatcggg aagaacccgc gaaccttgcc gccgaggggc
     4681 tgatgctcga cacgtcggcc ctgacgatcg ggtttgcccg ccggttcgcc gagtacaagc
     4741 gggcggacct catattcgag gacctggatc ggctcgacag tatcgtgaac aaccgctgga
     4801 ggccggtgca gttcatcttc tcggggaagg ctcatcccgc cgacgagaag ggcaaacgta
     4861 tcctgcagag gatctaccag tactcccagg atccccgatt cgggggccgg atctccttca
     4921 tcgaggatta cggcgagcag ctggcgcgct acatggtcca gggcgtcgat gtctggatga
     4981 atacacccgt cccgctcatg gaggcaagcg gaacgagcgg catgaaggcg gggatgaacg
     5041 gggtgctgaa cctctcggtc ctggatggct ggtgggtcga gggctacaac ggcaggaacg
     5101 ggtgggggtt tgagggtcac accccctatg ccgggaacaa ccgggcggac gcggatacga
     5161 tctacgacct tatcgagaac gaggtcgccc cgctctacta ctcgcggacg atggacaacg
     5221 tcccgcataa atgggtccag atgatgaaag agtcgataaa gagcatcgcc ccggagtact
     5281 gctccttccg gatgctcaag gattacatca ggagctacta cccctcggtc tgcatgtatg
     5341 cggccgaacc ggagagacaa ctggccggca tcgagggggt ctgcctcccc acgaacccct
     5401 ttcggtaggt tcatggagaa tcgggggcgt acaacagatg catgctgccc cgcccgatga
     5461 cgatccggcc tgagacgaga gaggattacc cggcggtctg cgttctggcc ttgcgtgcgt
     5521 tcgtgctccg tgatgagagt tctgacgcat gacggggagc cgatctgccc ggaaggtgct
     5581 gattgccgtg gcagtcgcgg cgatgcttgg cgcagcactc atcgtcgctc cgccggcgac
     5641 gccggcaacc gtgctcctcc tctttacgat cggcatcgtt gccgggatct tcggcggcct
     5701 catcggggtc ggcggatcga cgatcatgct cccgatcatg tacttctatc tcgggtttcc
     5761 cgaacccatc gccgtcggga ccggcctctt cgtggtcatc ttcacgtcgc tctccggcgc
     5821 tgcgggccac ctggtgcggg ggaacctgga tcgtcgtgtt gcaggctgga tcgccgccgg
     5881 cggcctggtg ggggtcatct tcggttcctg gctcttctcc ttcctggtcg gctacacccg
     5941 cctcctcagc ctcgtcctgg gattggtctt cctggtcccc tccctcggca tgatccgtga
     6001 cgggctccgg cccgccaccc gcccggagcc cgggtgcatc gggggtacgc cccccggcca
     6061 ccttctcttc ggcaccgggg tcggcgtcct gaccggtata accggacttg gcggcggcta
     6121 cgccctcgtt cccgggctgc tctacatatt cggcgccccg gtcgcggtca cgatgggaac
     6181 gtcgcttgca tccatgatac cgatggcgat ccttggcggc ggtatcaagc tcgtggaggg
     6241 ttacgtcgcc gtcggggcgg gattgaccct tgccgccggg tcgatcgccg gcgcccaggc
     6301 gggggctgcg acgattcacc ggttccggcc ggagaccctg aaactgatct tcggtttcta
     6361 cttcctctac gctgcaatcc ggtttatcac cgggttcttc ggcgtcgggg tgccgtgagt
     6421 caaggttttt agcgggcaac cgcccatatc ctagtatcga tacgcagtgg gagatcggat
     6481 atggaatgga aagatcttct ccccgttctt gccggcgggc ttgttatcct ggccgtcgcc
     6541 ctcgtagtga aacccgcgct cgtcggagga cctgcaactc cccccggttc gctcccgccg
     6601 gcatacgtcc cgcccgctcc tctgccggcg gcgacgccct accccgaaga cggtgcggtc
     6661 tacacccggg atttccgctg gacggcgatc gacggcggcg tcctgacgac agaggtcagg
     6721 gttccggagg cgctctttcg cgatcaccgg gagacgccgc ggatcgaaga tcgtctcgcc
     6781 tatggacgat atgtgcttgc cgatgccgat cggccgattc ttgaagatct cgtccgccag
     6841 atcgctcccc ccgttgccag tccggatgca gagtactatc ggctgatgaa cgttgtcttc
     6901 tttgtccagc agatccccta cgagtatgat aacagcacga actcttacat cgaaggcgtc
     6961 ctcccccggc acgccctgca catcggtgat ggtatcgagt atcagaagta tcctgcggag
     7021 atgctcgttg acgggaaagg tgactgtgag gatgccgcca tcctgatggc ggggctcatc
     7081 gatgccctcg gctacgatgc ggtgctgctg agatatcggg gcgacacgaa actcggtatc
     7141 gcgggccata tggctctcgg tatccagatg aacgagttca atccctacta cgcgaagtac
     7201 acgccgaggt acttcgagca cggagggaaa cactactact acattgaggg gacggactat
     7261 ctcaaattct ccgcggggaa cgtgacgtcc tgggggaaac cgttccccat cggggatgtc
     7321 gacggcaccg gcatcgaggc cgtgaggtcc agaatcccgg agatcatccc cctgcgttat
     7381 acccccgcac cggccgaata ccgtatccgc tccgtcgggc tgccggcagg agggggatga
     7441 ggaccatgga tatcactgtc ccgaaacttc cgactctccc gaagcttcct gccatcgcga
     7501 ccaaccggcg gcaggcgctc atcgccgtcg gggcggtgat tgctgtggta gccgccgcct
     7561 tcatcgggtg gacactcttt accgattgga ccgaaagccg gcaggttgcg gtctttggca
     7621 gccacgtggc ggcatcggag gccgaccttg cccgggtctc cgcgaagata tcctcccaca
     7681 tgagttctcg cccggaggcc ccgtcgattg cggaatccga tgttgtaatg cgggagtttg
     7741 cggccatagc gacgtacggg caggcggtca cgacctacca ccggcggatc atcggggccg
     7801 acgaggtccc tgaagcgtat attggagcgc agtcggccta catccgggcg ctcgattgcc
     7861 tgaaccgcgc gttctccctc tgggcgtcgg ccgcggccgc ctacgacgca ggagcctata
     7921 cctcggcaaa agatgccctt gtaaaggcag atcaggcatg gaaggagtac gtcgccgcga
     7981 caggagacta caaccgggag ctccggagag cagaagaagg ggaagaggcc cctcccgtct
     8041 agacgagata cttctccggg atgagaatgg tgagctcgaa cgactttacg gactcagaac
     8101 ggatgatgaa gtagtaatcc cggaatcctt cgaggatcat gaatcgctct ttcttctctc
     8161 tgggcattcc gccctcgtac ggaaagctgt ttcgggagta acgtatggtc tcgatgacgg
     8221 cccggttggg gtcgtccttc tcatggacat cgatcgtgag ccagggatgt tcgcccatcg
     8281 gggttatgct cacgttgaag gcccagtagg ggaagggtat gtggtaggtc tcggtggcaa
     8341 gaacgccggg agggtaattg atggttgccc tggcgtaatc caccatcttc tcggagacct
     8401 tgatgtcgtc cggctgcttt cctattgcga tcgtgggcgt cggggtcggt atccggtaga
     8461 ccgccgggtc gacgaactcc accttcctca cttcctgcgt tgcgtttccc cggacgggcg
     8521 cagccggcgt gggggcgctc atcccgtcac cggacatctg cggctgcgct gatgacggcg
     8581 ccggggtgag ggaggctggc tgcgtctcgc tcctctcgcc gtctgccgtg caaccgcagg
     8641 cgaggatggt cgccgtaaga aggatcagga gcaggatatg gcgcccttgc atgatgatct
     8701 cttgttttgc ggcgttatta gaagttactc tttcggacgg ccgaaaaaac ccccgtacac
     8761 gtttccgggg ggtgtcggtg catccggacg ggattgcaaa aagagatctg tgccggtggg
     8821 gtgcttcacg ttccctcaag cgcctccatg gcaaggcccc tgaacggctc gccctcccgg
     8881 tcgaacacct tccacacctc ggcgatgccc gccgggtcgc ccagtttcag cagggctgct
     8941 gcggtcaggg cccggcagca ctccgactgg ccgcttttga gggtgccgac cagtggattg
     9001 accgcccggg ggtcgccgat ctcggcaagc gcccagattg ccgggttctt gagttcgtca
     9061 tccgcgacga gcacgacccc gatgagcggc tccaccgccg gctccccggt tcgggcgagc
     9121 gccatcgcca cggtccagcg tgcatcgctg ccggcggcaa gcagggtctc caccagcggc
     9181 ccgaccgccg gcgccccgat ggccccgagg gtgctgattg cctgcaggct ggtgtaggtg
     9241 tcggcgttca gcatgacgtg tacgagctcc cggacctctg cagtggtctc ctcctgtacc
     9301 tctctcctct cgatggtttt ttgcatctct tcaacctcct ggtatggacg caggatgttc
     9361 tggatgtaaa ataagcgttt acgaaccaga atcgctgtgc ggtactgcag accggcactg
     9421 ccgggcatag atttttatat ccccggagct acggctatgt cggagcgttt cgtgtcttct
     9481 ccggtttccc ctgttctttt gggagacgtg tcgctgccgc catcatcccg atcaccctgc
     9541 cgctctcggc cacgataggc gtcccgagga gatccaccgt aaacctcgtg ccgtccttcc
     9601 ggaggccgac gagctcgccg tgccaggctc cgccgccgat gaccgcccgg atgatcatcc
     9661 ggatcttgtc ggggtgctcc cagaaccctg agaagggcga tccctgcacc tcaactgccg
     9721 ccgggtagcc ccagagggcg aggtacgcgg cgtttgcgta ggtcaccacg ccatccagag
     9781 caaggagagc gaccgcccgc cgtgtggagg ctatcgcccg gtctttgatg aggagctcct
     9841 gctcctgtcg ccgcaggtcg gcgatatccg cgacgaggag gcggtaggcc cgggcctctt
     9901 cggtgatgtc ctcgcaggtg acgtactccg tcccgtcggg gagggtgacg gctcgggaga
     9961 ggatcgtctt cttcgccccg tcccgggatc ggactacaaa cgtccggggt ctgatctggc
    10021 ccggggccga ccgctcaagg tccgatcgcc aggccgcgac ggcttcctgc cagtcgctgt
    10081 cgtccgcgaa gatctgccgg acccattcgg tggccgtgac cgccctgccc gtctcgtagc
    10141 cgaagatctc agagaatctc cggttgcagt agcagaatcg cctctcgtga tcgatgagcg
    10201 cgatcccgaa gggggatgca tcaagcatgc tccggaaccg caccccgctc aaccggagcc
    10261 gctcctcggt ctcgcgccgg gcaatagcga tggagacctg gcggatgaag gactcgatga
    10321 tctctttgtt ctcgacctcc cttccgggag ggaggaagat acctgtgatg ccgtagagac
    10381 ggtcccgcca gaccagccct gtgaggcaga gcttcacgat attgaaccgt tcaaggatcg
    10441 cctcgcagat ctcctccggg atcgcccgaa agcagatgtc gtagaacggc catgcggatg
    10501 gttcggggcg gagaacgaac tcctgcaggc cgcgcatcga gaggggggtc tggtgatagg
    10561 gagcttcgaa cacgatggtg accgggagga tctggccgac cgggttccgt ccaaggagtt
    10621 ccgtgagccc ctcccggaac ccctcgcccg cggtcgcccg gatagcgaac tggtgttcgg
    10681 cctcgctgta ggagaggatg taggcgtacg ctcccggcac gagctctgcg aggcggtcgg
    10741 cgatgtactg gtagatgtcc tccaccggtt gcaggtcaac gagatctatc gctgtccggg
    10801 cgaggaactc cgtattcctg atgtatcgct tccgctcggt gatatcctcg aggatgaggg
    10861 tgcaccctct ctccccgccc tcgaacgtcg tcgggatcgc ctgcatctgg tagaaggact
    10921 cgctgccgtc attccgccgg tgggggatat cggcgatcac ctgctcccgc tcgaggttct
    10981 cgacgagggc gagcgatttc agcgaagaaa cgaccggaag ggccgcttcc cggaggttcc
    11041 ggcccacgag ttcctccttc gtacgcccaa accgtctcag gcactggtcg tttgcctgga
    11101 cgatcgtgag gtcggcgtcg aggaccagga tcaggttttt cgtgaaacag agaaacgctg
    11161 agaggggtac gcgttgcgcg acagaataga cctttgcgtt gccgacaagc cgggcctcga
    11221 cctgacctgc cgcctgcatc acctcaaggt gctttgatac cgagttccgg tttgatttga
    11281 gggtctttgc gatctcggtg atcgtcatgc ccttgggccg gaacttgagg ctccgcagga
    11341 tccttcccgg gatgtcctgc tcagattgca tacaaacaat gacagctaac accgctttat
    11401 agcatatata ttttcctcac attcccgggg ccgcccccgc ccccgggacg cccaccggcg
    11461 atggatggcg gagcttccgg caggggatgg tcgccggtgt gccggcaggg tcgggcagag
    11521 cccacggctt taagggggga cgacatatcc tgtacgaaag gcccggagga caccgaccat
    11581 ggaggagcat gagctcgtca gcataaccca gggagggttc gcgatcatga acccgaccac
    11641 gcccgagaag gtgctcgccg ccggacgggc ggcggggctc cggaaggggt cccgcgttgt
    11701 ggagatcggg tgcgggaacg ggacgatcct cgcgctctgg gggcgcgagt acggtgcatc
    11761 cggtctcggg atcgagtcgc gggaggacgc ctgccgccgg gcggaggaga cgttccgggc
    11821 ggcgggaccg ggcggccggc tccgcgtccg gtgcatggat gcccgggagt acgtctccga
    11881 cgaaccgttc gactgcgccg catcgatcgg ggcatcccac attcacgggg ggtttgcggc
    11941 gacgctcgac gccctcctcg acctggtcca cgacgaagga tcggtcgtca tcggcgatcg
    12001 tttctggcga tccgaccgta tcccgcctga atttgcccgc gcatggcccg acgtcccgac
    12061 cggctacgag gtcctcggca ccgcccggga ggccggtctc gatctcgccg ccgtcatccg
    12121 ggccgacggg gctgactggg accgctacga gtcgggaata tggcagtcgg tcctctcctg
    12181 gcttggcgct caccccgatc atcccgaccg cgactccatg atcgactacc tccaccgtct
    12241 ccaggacgag tacttcggct acggccggga gtacatggac tgggcgctct tcgtcatggt
    12301 gcccgggttc tggtgagcgg acaaaaaaag atggttccgg ttacgcaccg gcgtatacgc
    12361 ctgagccgac gaaccactct ccgtccaccg gagcgacgta gcagagtttc agcccgtcaa
    12421 ccccggtatc ggggttatag taggtgatgt agacgaaccc gcccccgctc tctgccgcgg
    12481 cgctctcgag caggattatc ctgacaccgt accggtcttc gaggtcgaag cggttctccc
    12541 cgatgtattc cggctggtag gggagggcga gcgtcgtccc gttcatcgtg taggcgaata
    12601 tgtacgcttc tccgtcagca aaatccccgg agacatcgtt gaacgcagca cacgccgccg
    12661 ctcttccctg cttctgcgca tagtcccgtg cggccttcac tcgctggacg agcgtctccc
    12721 gaacgctcgt gttgaacgtg gccgcgacgc cggggatgta gacccctgag ccgacgaacc
    12781 aggtctcgtc cacggggagg acgtaaccga gtttgaggtc ttcggcgtac cctcgcgcag
    12841 ggtttggata gatatagttg atgctgccgc cgccttccgc cgcaagtctt ttcatgccgg
    12901 cgatgtaggc cacgccgctt acatcgtgca ccccgctccg gtccgccccg atgagcgcct
    12961 gctggaaggg aagggcgagg acggtgccgt tcatgtcgta ggcgaatatg tagagctcgt
    13021 ccctgacgaa ctccccggcg gggttgttga atgcagcaag tgcgtcggtg cgaccgtgct
    13081 cacgcgcgta cgcggccgcc tcgccgacga aagccttcat atccccggcg gtatccgcga
    13141 cgggcggcct ctcgccggtt gtggccggca cctcaccggc atcggcgctc cgcgtggcga
    13201 cgacccgcca ctcggtcccg ctgatcccgg cggtatccca gatcgccttt ttggtcacgt
    13261 ttcgttccca gttccgttcc cagaacgagt actcaagtga ccccgagggc tcggcgacta
    13321 tccgggagaa cgcctcctgg agtccggggg cctggtatac cggatctgca aagaggttac
    13381 gcccgatctc ctcaggtgtc gtatcgtaga ccaccagccc gtcggtctgc gtcacccaga
    13441 tgtcgtaggg ggtgccgttt atcaggggta cgatcgcccg gccgatgagg gcatcggagc
    13501 ggtacgcgac gctcacgaac ccgatgtact cccctcctgc tccaaagatc gggtaactct
    13561 gggcaacccc ggcatacccc tctgcaaggg gaaagacctc acttacgagc ggcacctgct
    13621 cttgaaccga tcgctcggtc tccgcatggg acgatatatc gctccccacc gtgccggcgt
    13681 agctctcggg cacggccatg gtcacgatgc cctctttcgt cgccaccagg gtcgactctg
    13741 tgtaagggtg gttcagcatc gcccgctgca ccgcctcctt gccggcggcg cttctaagcc
    13801 cggtctcccc gaggacccgg gcgctctcgc cgatcgaggc gttgatcgat tcgagctcgc
    13861 cgtttatcct ggcgacggct tccagaagaa ccgatttttc ggcctcttcc ggcggtggct
    13921 gactctctcc cacgcaccct gccccgagca ccacgacggc gacaaggagg atggcaatgc
    13981 atgcaggtgt tcgcataatc gcagttaccc cggggcaata ttaagtatgc tggtttttcg
    14041 cgatgctctt tgtgaaaggg cctcacctgt ggtcacgcca cgcttgagga tggcccgggt
    14101 gatgctgtgc cccgggtggg ggacgcccgg ctgacagggg gaaacaatcc gctctccttt
    14161 ataccgccgg tttcccgttt gtttcgtcat cccgggccgc ggggcaacaa cggtttcgga
    14221 agatgtggcg ccgttcaaaa ttctttcggc aaaatacggc ggaaatgagg gacaatagcc
    14281 gatacaaaat agaatcgcat aaataccgca tgcgtcatct aatgtttggg gaactactac
    14341 cccggtgaca tgagagaact gtagacgcga gtggagcgta tcccggaggg ttgcgctcgc
    14401 actcacccgt cgcgtgcggt gtttgccgcg ctcgggaggt cgggtgggca tccacctcct
    14461 gcacctcctt ctcgcgccgt gcgctctctt cttcgctccg ataagcacag cctgacctca
    14521 tcgcccacga gacggagggg aatgccccgg ttccgcgcga tcgccgcaag atcgtcatac
    14581 tcgggtttct ctgcaaccgg gcggccgttc gcgctcgcgg tcttcacgcg gatggggtac
    14641 tccctgccgc cgacgctgac cggcaccgtc tctttcctcc ggtccgcgac caccttccgg
    14701 aactcgcgca ccctgacccc gagggtcccg gtctcttcca tgagtatccg gatcaagcgg
    14761 ttttcatccg ccgccgcggc catcaccgat acgacgctga ccggccggtt cttcttcccg
    14821 agcgccggag tgacgaagac gtccgctgct ccctctgcaa agaggcgctc gatcgtgtag
    14881 ccgatcacct cgccggaagt gtcgtcgagg tttgtctcga gcatgacgac ccgatcctcc
    14941 cccgggggaa ggctctctcc ctccatcacc cggagaaggc cggtcggctc tccagagggc
    15001 cggatgccgt agccgacccg ggcgggggtc atggccgggt aaaggcttat ggagcgctcc
    15061 gtgatggcgg cgagcagggc tgccccgacc ggcgtggtca ggtccgcttc ggcgggcacg
    15121 gaggcgaagg ggaaccggtg gcgggcaagg atcgcaagcg tcgtcggcgc gggtccgggt
    15181 atatcgccgc agtccgtcct cgttatcgtg gcgccgagcg ccggaggcat cgcgacgatc
    15241 gttccctcaa agaattctgc ccggtcgagg aggaacatcg tcccgacgac ggagaagatc
    15301 gtctccgccg gcctgaagct gacggcggta aggtcgccga ggaccgaaga tacccgatcc
    15361 ctggcgcccg gcgagagccc cacatcatcc gcgactgcca tgcacgcctc ctggaagtcg
    15421 tctccggcca ttgcgccctc ttcccgggcc gtgatcgaaa gaagggtcgc cgcgacctcc
    15481 tcgccggcac ccaccctctc tgcttcatag cggaactccc ggcagatccc gaggtcccgg
    15541 atggcgcccg caagcgggtc gagcgcccgg ggttccccgg tgaggtcgat gagggatgca
    15601 agaagtatgc tcccatcgac ccccgagcat cccggatcga cgataaggat cttcatgttt
    15661 ggcggtcctc acgccacctc gtccggtttg ccgaggtttg cgacgatatc ctgtgcgatc
    15721 gtcgccaccc gctccgccac cgcccggccg ccggggatgg cgttcgtaag gcgtgcgcct
    15781 ttcgccgccc ctgttgccgc ttctgccggg ctggtgccga gcgcggcagc aacggcggcg
    15841 ggcacggtga tgttgaggat cgtgttcccg ccgtactgca gctcctgggt gaggacggcc
    15901 cccgcgaata tggcagtgcc catgcccggg tcatcccgcc ccttcccctc aagcgcatat
    15961 tccgggatca cccgggcggt gaggttctcg atatggtaac tctctccgtc gatcctgaca
    16021 tcataggaga ccttcgcatc aaacccccag aaccgcgcgg tgagcgggtc ggtcctccgc
    16081 gcgtggggcg caatctccgt gaaccggaag gtgatctcct ttccgacgaa tcccgagagg
    16141 atcgcagaac cccgcctctc aatgagagcc ttcttctcct catcgagggc tccggcagct
    16201 tcttcaagcg tccttccttc tttgagcatc tcatagacct tcaccgcccg ccggtagatt
    16261 gcccggttcc gggtcggttc gctcgcccgg atgcaggcat ccgtcaccgg accacggttg
    16321 atgacctccg cctttcgggt gatggtattc gtggcgcaga gggcggtctc ggggtcgatg
    16381 aaggactgca tcttgtagtc ccgtatcgcc gccgcggtac gttcgatgtc gccgccgttc
    16441 ccgatcagca gccggagcgc cggcagtata tctccggcga ggtgaccgag ggggacgttc
    16501 accggaccac cggagaaccc ggctccaatg gcggcggatt cctggaatcc ggcgaagagt
    16561 tcgttcgtga ccatcgatcc gatcaccgac ggtccgccga cgtctttcaa gatcaatccg
    16621 aagtcgccga cgagccgggc ggtctcgtac tcctccccgg tcccccgcat atggatcttc
    16681 ttcgggagcc ccgctgcctc gacggccccc tttccggcaa ggtaagagga gtcgacctcg
    16741 ccgaacttcc cgaactcttc cccgagcagg gcatccgggt ggacgagctc catcgtcacc
    16801 gccgccgcga tcagggcggg ccagagggga taaggggtcg tcccggctcc ctccatgctc
    16861 cgcatcagcg ccctggtccc gaccatcgcg gcatttctcg cgagttcggg tatgttgtag
    16921 tcctccccga gggcggcatg tcccatgacc ccgccgccgg cgacgaacgg cggcaggacc
    16981 gcgccgtcga ggcgggtcaa ccgttttccc atgagggcct cgtatatcgc gaggtaggcg
    17041 gggaacgcag ggacccggtg ggtgaacttg ttcgtcgtca gggcgatggc gcttgtccgg
    17101 tcgccgccgg catgcatccg ggcgatggcg ccgagcttcc ggttcccgag cgggacgccg
    17161 gcacgggcgc agctccccga gaagtaggcg agcgtggcaa cgataagcgc cgcgttcgcc
    17221 ggttcggctc caccccgctt tgcctcaccc accgccttcg cgatgacgga atctagcggc
    17281 aggctggggg ccgaagcatc gacgaccgcc atatccatga ggcgcatctc cggggcgccg
    17341 atgctgcaga agatatcccc cgcaagcgag ttcgccgctg caaacgccgg gataacgagc
    17401 gccccgtgtc cccgggtcgc cgcctccagc ttgtcgacgg tcatgtagtc gggctcatac
    17461 gtcacggcat gggccgcagc gaggagcacc cgctctcgtt ctatctccat gggtccggta
    17521 acctcccttc ttttacgcgt gaacaggagg tttcgggcat aaaaaggtgc cgcccggggc
    17581 ggtgccgggg gatccggcta catgtacagc gcctggatcg cgacgacttc agcgctgtct
    17641 tttgcctgtg cgtagtcttc caggggctcg gcgttcacct cgaggaaccg cagcccccac
    17701 ccgaacgggg cgaggatggt ccgggcctgc tcctcctcgc cgagtatgga gagcgtcgcg
    17761 gccatcgcct ccaccgatgt cagccggaac ggccggccga agttcaccgg gtttgcggcg
    17821 acgaggaagg ggagcgcccg gcggttgcgc caggacgcga cggcaccggt atcgaggacc
    17881 tcccaggagc agtcgagggc cgttatcgag gggagatggc ggtcggcggg ggagaccgcc
    17941 cgctccgcgg tcgggtcgag gaggagggtc gaacggggga tctttgcgat gttcgtgacg
    18001 atcgtggcaa gcccgcgccg tgcgagcttc tttaccgtgc acttccgggg gtcgcaggtg
    18061 ttgtcccggt aggcgtagag cggtatcatc tgtcttgtta tggtacgccg ccgagatctc
    18121 atcaatgtac gtactcgtcg tcccctgcat cgagaacccc gcctgccgtg cccgcggtat
    18181 aacgaccgat gaggatctcc gccacttcag ccgggccgtc gaacgctgcc ggcacttctc
    18241 gatcgatatg gtcccgctcc cctgcccgga gacgatctac atcggggcgg atcggccgcc
    18301 gggttcgtac ctcgaccgcc tcgcgacccc cgcgtttgcc gctctcctcg accggctcga
    18361 ggcggaggtc cgcgccgtca tccgcgagcg gggcgaggcg cctcttgctc tcgtcggtgt
    18421 ggactcctcc cctgcctgcg gggtgacggc cacctactat tcatccgtaa aacagcccgg
    18481 gcgcggcgcg ttcctcgccc ggttcccgga tataccggcc atcgacgtga aggagttcgc
    18541 ccgttaccgg atctacctcg caggccccct cttctccgag gctgaacggg cctacaacct
    18601 catcctccac gatctcctcg cagtgcacct cttcgacgtc tatcttcccc aggaggccgg
    18661 ggatacggac cccgcccggt gcggcgagga gcatggggcc atatttgcac agaacctcct
    18721 ggctctccgc gacgccgatg tcgtcgtcgc ggtcatcgat ggcgcggatg ccgactccgg
    18781 gacatcctgg gagatggggt atgcctatgc cctgaagaag aagattgtcg ctctccggac
    18841 cgatttccgg gtcgtcggcc accacgagcg ggtcaacctg atgctcgagg agtcggccgc
    18901 ggtcgtcacg aagaaggagg atcttccccg ggcgctcggg tcgcttctcc cggcgtcacc
    18961 ctgacgccgg ccgcctcttc tccatcgaga ggacgtttct gtcgtccctg cgggcatacg
    19021 ccacgttatc catcaccgtc cggatgaagt ggataccaag gcccccgatc ggccgctctt
    19081 ccgcggggat atcaaggagc ggcggcgggg cggtcagagg atcgaacgca acgccgtcgt
    19141 cggtgatcgt cacgcggacg acgtcgtcgt ccacggtgca ggcgatcgtg atcgctcctg
    19201 cctcccggtt cgggtagccg tagaggatgg tgttgctgca ggcctcgtcc acggcaagct
    19261 ggaccgaaaa gaccatatcg tcggcgctcc cggcgagtgt tccggcaagg aaatcggcga
    19321 tcgccgtgag agccccgagc tctgcccgca ccgtgagttc cgccatcaca ccacccggag
    19381 caccatgagc gtctggtcgt caaactgcgg ttcgtccccc gagaaatcag cgaccgcccg
    19441 gcggatacga tcgacgatct cccttgaggg gaggtcgcgg cagcccatga cggtctcgat
    19501 cagtcgctgc tcaccaaact gctcctcatg ggagtcgatc gcctcggtga ccccgtcggt
    19561 gtagaggagg acgaggtcgt tcttctcaag cgcgagggtc tcctccccgt agtcggcatc
    19621 cggcatgaca ccgaggatga taccggtccc tttcagctcg agcacccgtt cgctgcctga
    19681 ccggaagagg aggggagggt tatgccccgc gttgacgtag gtaagcgtct tcttcaccgg
    19741 atcgacgacc gcgtagaaga gggtgacgaa catgccggac tttgcgtctg cggcgatcag
    19801 ggcgttcgct tcgcggacgg catcacgggc gctccgtggg atcagagcgt tcgcccggag
    19861 caccgtccgc gagagcgcca taaagagcgc cgcgggaacg cccttccccg cgacgtcggc
    19921 gatgacgaag gcaattcgct gcccggagag ggggattgcg tcatagaagt ccccgccgac
    19981 ctcttttgcc gggagcgaga aggcggcgat atcgaggccc gggacatcgg gcagccgttc
    20041 ggggaggaag ctcatctgga tatcgtgcgc aatccggagc tcggccttct tccgctcgag
    20101 ttccgagagg taagcatccc gctccgcttt cgtctgccgt tcagtgatca ggttcccgat
    20161 gatgagcgcg aatatgaaga ccccgatggc gtttgcgatg atcatcggaa gggccacctc
    20221 tctgacgacg gtgagcgcct ggtcgaacgg ccggcagagg aggagggtga tgcccatgtg
    20281 aaacgcctcc atgccgatgg caaagaggac cgcgccgtgt atcccgacga acttccgtcc
    20341 ggagaggagg aagatcaacc ccccgaagag gcccgcaagg atggttgctg ccgagcacgg
    20401 gacggcggtg aaccccgcaa gggagaagcg gtaagcggcg ccgatcaggc cggcgccgag
    20461 ccccaccacc gggccgcacg cgagcccccc gaccatgggg ccgaggtcgc ggacgtttgc
    20521 agtggcgccg aggatggtga tgccgctctc ggtgccgtag atggagagcg ccccaaagag
    20581 gaggatgagg atcgcctggc tcttccaggt cacgaggccg tcgagcaccc gggtaaacga
    20641 cgctgtccgg gtgatcaggt aggcggccac caggatgacg cagatcatct ggaagagcac
    20701 aaaaaagaga tccccaaatt cggagaacgc catattgttc agggccccgc cgggaacccg
    20761 gcaagcgccg tcgcctcgtc gggggagagg gagaagatcc ggtgaaagcc cgccatcgtg
    20821 aagacctcaa gtatctgcgg ctgtgcacag gctatcctga gcgctccgcc atctttcttc
    20881 agacgtttca gtgcggcaag cagtacccgg aggccggagc tgctgatgta gtcgagatcg
    20941 cagaggttga tgaggagata tctccctccg gcatcgagca ccctgttgat ccgttcttcc
    21001 gctttctccg aagagcctgc gtcgagcctt cctttcaccg caatgatgtc gacggcgccc
    21061 gctctccttt cgagtatatc aacacgctcg ctcattgaat ggatattcga tctggtgaaa
    21121 gatagatgtt actctctgtt ccggggtttc tgagaaggaa gcaaaaaaaa tgagggtgta
    21181 ttgtcgggtg tgtgataacc atacggggta tttgggcagt cccggtgcgg ttgtgccgtc
    21241 cgcgccggtg tcctgttcca ccgtgccggt atgagtgttc agagaggtgt gcctgatcat
    21301 attcaccggg gtgcccccgg tgtggacgtg aatcttgacg tcggtgcaat cagcgtggtg
    21361 aatgttccgt tcaccgaccc cagccgttct tgtgccggtc cggaccacct gtgcccgtgt
    21421 ggcccgggtc gccggcagag tttccccttc tctgccgact ctggggatca tgcattgatc
    21481 gattcaggat atatactttt tctttaatgc cttgcattgg ggtgtggcat tgtacttggc
    21541 atcgtggcat gtaacactgc aaaacactat atcgtccggc gcgaaaccct gttcgcacat
    21601 ggatcccact accggcgcac tctctctcct gctcagcaat ttaaaagagc atccgcgggg
    21661 catgtcggtc tccgatcttg ctgccgccgt tggaatcaac cggaacaccg tctcccggta
    21721 cctcgacgtc ctcctcgtct cgggccaggt ggagatggag acctacggga aggccaaggt
    21781 cttctacctc tcgcaacgcg tccccatcgc cgccatgctc aacttctcat ccgatctcgt
    21841 gctgagcctc gaccgcgagc tccggattgt ccaggcaaac gatgccgttt gcgcgtttgc
    21901 gggaagggag cgggatgata tcatcgggaa cggcatcgag gcatcaccgc ttgcggcgtt
    21961 cgaccacccg ctgatacggg gccggatcac cgatgccctg aacggccgcg aggtcaccga
    22021 ggagctccgc ttcctgcggc acgaccggga actcttcttc cgtgccaagt tcctccccac
    22081 cgtcttcaac gacggggcgc cgggagtcac catcatactc gaggatatca ccgaggagcg
    22141 ccgggcggag gaggccctgc gcgagagcga actgctcttc cggagcctcg tcgagaacat
    22201 cagcgacctg atcctgaacg tcgacgagac ctttcggttc acctacgtca gcccgaaaag
    22261 ccgggagatc cttggttatg cgcccgagga gatgcttcac aagactccct gcgacttcat
    22321 ggcccctgaa aatgccgaac gggccagggc acagttctcc gcgctgctcg ccgacccgga
    22381 gccccggatt ctctttgagt ggacgatgct ccatcgggac gaaagcgacg tcatcctcga
    22441 cgtgagcggg acgccggtat acgatgtgat cggcgacttc accggctacc gggtggtctg
    22501 ccgcgacgtc accgagcggg cgcgtgccgc aaagcgggtg gcccagtgga agtcgttcct
    22561 ctactcggtc gtcaacaacg tcccgggcat ggtcttcgtc cgggaagtgg aagggaacac
    22621 gttcgtcttc gcaaaccggg ctgcggagat cttccttggc atgacgcagg atgagatggc
    22681 gggcaggcag gccgcggata tattcccgcc ggagatggcc gtcttctttg ccgatggcga
    22741 ccgggaactc gtggagcggg cggccgccct tgagaaggag atccggatat caggcgggcg
    22801 gacgctttcc atgaagaaga tcccgatatt caactcccgg ggcgtgctcc gttacatgct
    22861 cgggatcgcc gaggacatca ccgatcgttc tgccgccgag gaccttctgg tcgccgaacg
    22921 cgaccgggcg cagggctacc tggacgcggc cggcgtgatg atcgcggtga tcggggcgga
    22981 cgggaccatc gatctcatca accggcgggg aagcgagctt ctcgggtatg ccggggacga
    23041 tctcgccggg aagaactggt ttaaaacggt tgtcccggag cgcctgcgcg atccgctcgc
    23101 ccggaacttc gcccgcctcg tggccggcag gatcgagccg cccgccttcg aagagagccc
    23161 ggtcgtcacc cgggacggtc gggaacggtt gatccgctgg cacaacgccc tcctccggga
    23221 tgcagacggc aatgtcgtgg cgatggtgag ctccggggag gagcccgcgg ggtagctgga
    23281 tcctccctgc cggtatggga aatccggatg gattcggcca tacccgcgca tttgagggtt
    23341 atctcttaaa tacgccttat agtacataca ctatatggtg acaactttgg tggacatcat
    23401 ctcccttgtc cttatcgccg tcgtggtgct gatcgtcatc ggcatcgtgg cggtcatgat
    23461 aggcatctac aaccgattct tctccctcaa aaactcggcc gacgcgacgg tcgggcaggt
    23521 gaaggtcgcc atgaagaagc gactggatat gatcgagcag ctcctcggcg cggtgaagag
    23581 ctacgcggcg tttgagaagg ataccctgac cggggtgacc gcgatgcggg cccgcatcgg
    23641 gagcgccggc cccggcgact taaacgagct tgagcgcgag tcgcggtcgg tcctcggccg
    23701 gctgattgcc gtcgcggaga actatcccaa cctcaagaca tctcagacgg tgcaggacct
    23761 gatgggcgcg gtgaagggaa tcgaggatga gatcgcccgg caccgctaca cctataacaa
    23821 catcgcccag caatacaaca ccatgaccga cacgatccct tcaaacctgg ttgcgagcgt
    23881 catgggcttt aagaaactgg aatacctgga gttcggcgag gatatcgagc gggttccgac
    23941 gatagcgttt tgatagatca tgcgcgtgac tgaacagcag cagatcctca tccttgttgc
    24001 catcaccctg cttatcgggg tcctggcggt cgccggagct gtcgcgctcc ccggtctcct
    24061 ccagggagac ctggacgtcg ggcactacga cgccgtcttc tacgagaacg ggaccctcat
    24121 cgagcgctac acctacgatg tccgcgccgc aggcgagtac cggatgctct ttcgctactg
    24181 ggatgcgccg ctcaccttcg cggccatcga tcagccgcac gtagagttcc tcggtatgac
    24241 cgcgccgccc ggtgtcatcg gctacgccag ggattccgga ggcgaggtcc ggacggccgc
    24301 cgggagtgcg accccatcgg atcttgccac gatccggaac cttgcactcg atagcgaagt
    24361 gggtatcttc aaccccggct actttgcccc gggcacctac acggtggaat accgttacct
    24421 ggtccggccg ccggtcgagt acgacagcca gtgggcgcac ctgaacctga aactcgtgga
    24481 cgagcacgtc cccttccgga acctccggat caccctcccg tttgccgggg atatcgagga
    24541 ggtctacacg caccccccta cccttgatat cgagcgaacg gcggacgccg tcgtcataac
    24601 ggggagcgca tcccagaacg acgcgctgaa cgtcgaactg atcctcgatc cggcgttcac
    24661 cgagaaggtc ggcggatttg gggagttcgt ccccgacgtg cggcagaagg cggccgacgc
    24721 gaatcgctgg ccccccctcc tctacggggc cgcgaatatc ctttacggcc tctcgatcct
    24781 cctcgtcctc gcgacgccgt tcatcctcct tggcgtctac ctccgctacg gcagagagaa
    24841 accgttcacg gtgccggaat acctgagttt cacgccgaat accgcgctca aaccctggca
    24901 ggtgaacctc ctcttcaagg gcgacgccct tgagttcgac gataacgggt tctacgcgac
    24961 ggtcctcgac ctccaccggc agaagaagat cgtcgtgacc gaaaaatcgg gcggcaacgg
    25021 tgtcacggtc cggatcgtct cgggggagtc ctccgacccc tacgagcagc gggtgctcaa
    25081 cttccttgcc aacatagccg acgaccatgt cgtggatacc gccgatctcg agacgttcgc
    25141 cgagaccgcc cgacggagcc cgggttacca gcaccggatc ctgcagtacc agcagtcgct
    25201 tgccgctctc acccggaatg tggatacgac gcttgcccgg cagtatgtca gtgatgggag
    25261 gggactcatc ctcccgctca tcttccttgg cgccatcccc tgcgggcttg cgatcctcac
    25321 gatcatattc gcgccgggcg cggcctacct cctcataccg gccgggatcc tctctttcat
    25381 cgtcgcggtc caggtgggga tagcggccct cttcccctcg accctctttg gggtctggaa
    25441 gggcgatcgc taccgggaga agctcgagtg ggatgcgttt gctcacttcc tctcggacct
    25501 cgctctgatc cggcagtact cccctgccga cctctcgatg tggggggagt ggctggtcta
    25561 cgggaccgcg ctcggcctcg gcgataaggt cgagcaggcg atgaagaact taaacatcag
    25621 cctcccggac gtcgggatgc cgctctactc gaacatgccg gtgatctttg ccccgatcgt
    25681 cttctactcg ccgccgtcgt cgggcggtgg ccatggtgga ttcgggggcg gcggatcctt
    25741 cggtggcggc ggtgggtttg gcggcggcgg cgtcggggga cgatgatacc cttttttctg
    25801 ccccgccggc cggataggtc cgaatctctt catagttaag gtaatctact tacatccggc
    25861 gcgagaatgt acgggtagtg atgcctatga ggggatccta tctgaactat tcagccgtcg
    25921 tcctcgcgct cctttcagtc gccgtcgcct gcgggtgcat ggccggcggg gagaaggacg
    25981 gtactcgcga ccgcatatcc ggaaacggga cgatcacgta tctcgatctc gagggcggtt
    26041 tctacggtat cgtcgccgac gacgggagcc gttatctccc cgccgacctt ccggcggact
    26101 tccggcagga tggcctccgg gtcgcgttcg tcgttgatag agcagaggag acagcgacca
    26161 tccagcagtg gggaacgccg gtcgatatcg tcagcatgga gaagggcgac gcccgccgga
    26221 cggtcacggc gaacggaacg gtcacctaca tcgatctcga gggcgggttt tacggtatca
    26281 tcgcggagga cggcatgaac tgtcttcctg cgaacctccc ggaggagtac cggatcgacg
    26341 gtctttctgt gcggttcagc gccgacctgg aggaagatgt cgtggggttc cctatgtggg
    26401 ggatacccgt gaatatccgg accatcgaac cctccggcgg tgtccgtctt gtcgccggga
    26461 acgggacgat cacctacgtc gatctcgagg gtgggtttta cgggatcgtc gcggatgacg
    26521 gcgagcagta tttcccgctc ggcctcagtg aagcctggct ggtcgacggc atggacgtgg
    26581 cgttcgttgc ccgggcaagg gaggatgtcg cggccatcgg gcagtggggg gctccggtcg
    26641 atgtcatcgc catcgataag gcggggagcg caacgtttgt cgctgaaaac ggcacggtca
    26701 cgtatatcga cctcgagggc gggttctatg ggatcatcgc cgatggcggc cggcactacc
    26761 tcccgctcgg gcttgaggag cgctatcgcg tcgacggcat gcggatcgcc tttgcgggga
    26821 agatcgcgcg cgacatcgtg accatccagc agtggggaac accggtgaag atcctcgccg
    26881 tcccgtgggc ctgctcatcc tgtggcggga gcgccggcat cgcaaaccct gccgcggcct
    26941 ggtgcctcgc gcagggccac gcctacgaga tccggaagaa ccccgacggc agcgagtacg
    27001 gtgtctgcat cttcgcaaac ggcaccgtga tcgatgagtg ggactattac cggcagagcc
    27061 actgaggctc tctacagggt tctccccctc cctctctttg cgcgaggccg tttcatcgct
    27121 cataccctcg agatcccgcg ttgccgcgaa gggtgcgaag tcctgcccgg gcacctgcac
    27181 ccccccttcg cgcgagcccg tacgactgtc cctctccccc cggtctgaga cgggcggctt
    27241 tttcccttcc cgcgccgaaa gga
//
LOCUS       DAOSEE010000011        26680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_840_length_26680_cov_209.141221, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000011 DAOSEE010000000
VERSION     DAOSEE010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 26680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26680
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_840_length_26680_cov_209.141221"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1028)
                     /locus_tag="PLI76_01715"
     CDS             complement(72..1028)
                     /locus_tag="PLI76_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition protein"
                     /protein_id="HOI60777.1"
                     /translation="MYESIVPTVMLAADLLGEMVPMMIIGVFIAEVLVALNIAGKIAS
                     LSRPLTSFACLSEECGTSFLMAFVSPPAANAMLLDYHTKEIISQKELIIAATMNSFPT
                     VVMHWRYLLPVYVPLLGIPGLIYFGLLMLAGLVKTLVIMIVGRMTLTPAPRSPAAPAE
                     VSAVTLREALGTACTSSKKILVRLLKITVPTIVIVAFLINAGVFDRLAVMMQGAGGVF
                     PVPAEGFAIIAAQFGSFVAGASVASALLAAGDMTWQQIIVTLLVGNVLTSVTRSIRWL
                     GSSYIAIFGVRTGAEIMFVSTALRNGIMVVLVAVLAYGFGWG"
     gene            1231..1365
                     /locus_tag="PLI76_01720"
     CDS             1231..1365
                     /locus_tag="PLI76_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60778.1"
                     /translation="MPTCSDCALYTRKTATSGECSINGPVPADRDAGRCPSRTFRPRG
                     "
     gene            1661..3586
                     /locus_tag="PLI76_01725"
     CDS             1661..3586
                     /locus_tag="PLI76_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOI60779.1"
                     /translation="MDVTQDSSGPLAVVPRSLFRDYREFLLTPGTIFTLVSSILLAIA
                     VLYQVSGLFIDAAPEAYADRIYLAAALVGSSFIWYSAVQGIREGDFTADIPVSLATAA
                     AIAIGQYPAAAVVAVLLLLGGLLEELVAARADRALDALARLLPDRVTVRRDGKDLFIS
                     PDELIVGDLLIVRSGERIAVDGEVLFGAAAINQSAITGESVPAEKQAGDTVFAGTLNE
                     NGAIEVLATRVGTETTLGQIRRLVADAQEEKAPVERVLDRYAKLYTPAAIILGLLLWW
                     WSGNVLQAITMLIVFCPCVIVLATPTALVASIGNAALRGSLVKKGAAVETLAHVDIVI
                     FDKTGTLTSGELHLADVVPFDDVLETSLLGYAASAEKFSEHPVGRAIVRAAAGREIAV
                     TDPESCELLTGLGVKARTGGHAVLLGRPKLFSDMGITLPKTVLWEINARACEGMSVIV
                     VMIDHAVSGLLVFEDTLREHAQETVAALNQAGIRTVIVTGDNRETAARIAEATGISEM
                     HAEMMPEEKVGIVKRFQEAGHRVAFVGDGVNDGPALATADVGVAMGNAGTDVAIETAD
                     VVLLSDDLLKLPRIIDTSRKALQTIRQNLVFAVGVLLFAVCLTVFNILTPVTGALLHE
                     LSSIPVIANSARLIGMK"
     gene            complement(3777..4181)
                     /gene="tsaA"
                     /locus_tag="PLI76_01730"
     CDS             complement(3777..4181)
                     /gene="tsaA"
                     /locus_tag="PLI76_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HOI60780.1"
                     /translation="MIVDLNPIGHVSSPYKEKKDAPRQGRLTDTEATIVIDDHYLPAL
                     FRVEEKKHLFVLCWFDRADRTALRATPPHNPVEHGVFATRSPNRPNPISLSLVDVIDI
                     TGNTIRVRGLEALDGTPVLDIKPYSREIDCPE"
     gene            4537..5118
                     /locus_tag="PLI76_01735"
     CDS             4537..5118
                     /locus_tag="PLI76_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOI60781.1"
                     /translation="MDDATALMLNHVRAALPASGAHGFDHTLRVVRLAEAIGAREGAD
                     MAVLIPAAIFHDIARPVEEETGAPHEEAGARMAEAYLCSISYPADLVPGILHAIRAHR
                     YSSGVDPGTLEARVLSDADNLDAMGAVGIARTFMQAGEKGGGILDATDHIRAKLVNLR
                     GRMYTDTAREIAGKRHALLVAFLNALDDEVRGP"
     gene            complement(5115..6446)
                     /locus_tag="PLI76_01740"
     CDS             complement(5115..6446)
                     /locus_tag="PLI76_01740"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOI60782.1"
                     /translation="MPGGAKRSKGLSLSAAVAFAVGNMIGAGVFVLSGLIIAIAGPSA
                     VISYIICGLAVAFSGLSYAVLASIYPEDGGGYLYARRILGPYPGFLAGFAMYISITIA
                     TAFVLLGFGIYANLLTGASLDPRYAALAGLALLAALNLRGAAEAGRVEIGLVAAKVAI
                     LAGLILVGLTHVQASDFSPFLPGGASGMLRGVAMIFFAYIGFQVVAMMGGEVKDSAKN
                     VPLATLASIGIVALIYTGVVVALLAARLPSYGGESVFDAAVVYLGPIGGVVVALAALF
                     STLSSANANILGASRVILEMASEEQIPARFARLRNGQPANSILLGAGITAILILAGNL
                     SFIVELTNVTILVAMMLVNVSALILTGRKTLVSPEKNRFVIPFGPVFPLLGAASCAAM
                     LVTLAPSVLGLGIAILLAGSLLLVIEDVPERKTIVRNIRLLLRRPVERDGG"
     gene            complement(6591..6920)
                     /locus_tag="PLI76_01745"
     CDS             complement(6591..6920)
                     /locus_tag="PLI76_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HOI60783.1"
                     /translation="MREIAWITLFFAGLLETGWALGLRYTEGFTKVGPSVATLIVMGG
                     SVYLLSRSLTSLPLGTAYAVWTGIGAVGTVIAGIILFGESRSVIRLLCIVLIVAGIVG
                     LRFFSDT"
     gene            7032..7259
                     /locus_tag="PLI76_01750"
     CDS             7032..7259
                     /locus_tag="PLI76_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOI60784.1"
                     /translation="MAKVTIERPECISCESCWTVCPEIFEQDPADGLSSIKKEYRVND
                     NPAEGEVPDDLIDCAIEAADACPVAIIEVEG"
     gene            complement(7600..8637)
                     /locus_tag="PLI76_01755"
     CDS             complement(7600..8637)
                     /locus_tag="PLI76_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOI60785.1"
                     /translation="MVKELLRKLSDAHGVTSREGNIRDIVRAELAGVVDEITEDTMGN
                     LIATKRGDGFSIMLAAHMDEIGLMVQYIDEKGFIRVVPLGGWFGPVLYCQRVVLHGTK
                     GPVKGVIGAKPPHVMKDEERKKEIKIEDMFIDVGAASAGDVADLGIEIGTPITIDREY
                     TELAGNRVTGKALDNRVGVAMLIRALQQMTSPHTVYAVFTVQEELGLKGAKVSAYTLN
                     PDCAIATDVTIPGDHPGIEKRDASVEMGKGPVLVLVSASGRGLMADPRMTAWLRETAE
                     KNNIPCQLEVGTGGNTDATIIHLERGGIPSIPFSIATRYIHSPVEVVDTADIEAGVRL
                     LVEALKGKPAL"
     gene            complement(8645..9751)
                     /locus_tag="PLI76_01760"
     CDS             complement(8645..9751)
                     /locus_tag="PLI76_01760"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOI60786.1"
                     /translation="MNTHRYADRVRSVEMSGIRKLFDAGGPNAINLGIGQPDFDTPGH
                     IKEAAIAAIRAGKTGYTPNAGIPELREAICGKFARENDLVYRPEQILVTAGGSEALHL
                     VMQALVNPGDRVLFADPGFVSYAALATFAGGRPEGIGLDATLHIDVERAKEQMDGARL
                     FVLNTPGNPTGAVESEESVRALVEYAGDRGVTVVSDEVYEHFIYEKKHVSAARFGEDV
                     ITINAASKTYAMTGWRVGYLAAPEDYIPECLKIHQYAQACATSIAQYAALAAYTGDQS
                     AVARMREEYRARRDLLYAGLTGLGFDFPRPEGAFYMFVPMGQTLIQRAVEAGVIIVPG
                     AAFGTRAPDHARFSYATAQENLSRAVGRIGALME"
     gene            complement(9760..10359)
                     /gene="hxlB"
                     /locus_tag="PLI76_01765"
     CDS             complement(9760..10359)
                     /gene="hxlB"
                     /locus_tag="PLI76_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HOI60787.1"
                     /translation="MTDHPVKNMMRLMATKIRTIADVMSDDQIDALLNEILNANRIYT
                     MGAGRSGLVAKSFAMRLMHLGLSAFVVGETVTPAMKPGDVIIVFSGSGATKTVADISE
                     TAKEIGGRVCLITSKKDSRIGRIASCVVVIESQRDKVADESAEFEIRQMMGEHKSFAP
                     LGTIFETTAMVFADAIISHLMEITQCRPEDLQCRHANIE"
     gene            10873..13488
                     /locus_tag="PLI76_01770"
     CDS             10873..13488
                     /locus_tag="PLI76_01770"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOI60788.1"
                     /translation="MAGQEGRSDEEIADLLDENVREWCRRQLDGRFTPPQRMAVPLIH
                     DNRNVLICSPTGSGKTLAAFLAIIDDLVVRARAGELEDTVYCLYISPLKSLANDIHKN
                     LSLPLQGIEAIARERGVRHTPIRHAVRHGDTPKAEKAKMARKPPHILNITPETLGILL
                     NSPKFRESLRTVRWVVIDEIHSLAGSKRGVHLSVSLERLEDLVKEAGGGFTRIGCSAT
                     IEPLDEVGRFLVGVGREVSIVDTRFAREFDLSLICPVPDLADTTPEDLSRHLYSTIHR
                     LVGEHENTLIFTNTRNGAERILHNLRARYPEYYTEENTGCHHGSMGAEGRLAVEEKLK
                     AGKVKAVTTSTSLELGVDMPHVDLVLQVGSPKSVAALLQRIGRAGHRLGEVVKGRIVV
                     LDRDELVECAVMLREGQRGFVDRIHIPENCLDVLAQHVFGMALDGSRRIDEMLAVFRR
                     SHCYRDLEEADLASVVRYLAGEYAGLEERHIYAKIWHDAATGTVRKRGRNARMIYFLN
                     SGTIPDEFSCDVLTRDGVFAGNLDEKYLERMNKGDVFVLGGRRYRFAYRRGGKLYVDP
                     TGDRPTIPSWFSEKLPLSFDLGLHALRFKESMLRAMEAMGTEDLVDALLCEYPIDENS
                     ARSICAIFEQQVCYLGEEAVPTPGRIVVEEQVDRENRRRLYFFMTNYGLRFNDGFSRI
                     VAFLIARDVTTNVSVGISDTGFLVSIPLEKRADPMRLLRGIRAGECRDILKEAVGGTQ
                     LEKRVFRINAARSYMILRNYSGRKRSARRQQVSADMLISFAKRLPGFAVMRETYREVI
                     EDRFEVANIEKIVGEIGAGEIDIALTRTASPSPLAFGIATLGISDAIYAEDRLSILRE
                     FQRRVMSSIGSGAAA"
     gene            13485..14201
                     /locus_tag="PLI76_01775"
     CDS             13485..14201
                     /locus_tag="PLI76_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOI60789.1"
                     /translation="MSAVSEADLLSRFGFYRSALYIRDIDTCVASDIHIGLAETLYRQ
                     GLHFPLHEEETLIERFEAILDRFNPAVFVFDGDIFHAFDRVSRSVVETFEAILATLRS
                     ECEVVLVTGSHDTMLPAVAGGTVERYDRGGYTVVHGDRPVGDHGTLVIGHDHPAIEID
                     MARFPCFLYGEGVFGGKDLVVMPAYNPLSPGVTINHMQRRDFLSPILRRVDAGSLQPV
                     VEVDGETVVLPPLAGIRRFA"
     assembly_gap    14867..14966
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            15342..18920
                     /locus_tag="PLI76_01780"
     CDS             15342..18920
                     /locus_tag="PLI76_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOI60790.1"
                     /translation="MTKVTHLALLCAFVAIGLLISPVMAVEAASGEHIYRYLDHAQDH
                     PDVDGNMIVWEDDRDGDKDIYFGTIDGFRESPGYTGERITADPARQERPSISGNYIVW
                     QDNRHGNPDIYLYDRLNATETQLTTCTGRQWLPAISGNYVAWYDDSSGRTTIVLYDIA
                     GKTVKDVIDCDARTTIPGASIEFKPALSEKYVAWVEDEDARVRYYDIAAGTIAGTVST
                     SMAVQCWPSLSGSLLVWEDYRDGNAEIYMFDLDNPSGNEQQITSDASDQVAPAISGRI
                     IAWEDNRDKGPDETDGRGIYLYDLASGKELSVKLPDENYDEHFYPAVSGNTVVWQRGA
                     TPNSNLYIFVYEPDVPVDPALTDITVTPSSATLMVGETKGFVATALDQDGNAMPGIDI
                     AWSSSDPTVGTIDDDGVFTALVAGTATVTAASGDIAGTATVTVSDEEPVLTGLNVTPP
                     RATLAAGDSLKFIVTARDQNGNVMPAGAVAWTSSDPAAGTIDDDGVFTALAAGTATVT
                     ATSGEVAATAAVTVIAAEPEFGMVVLSPPEVTLETGDTLQFEAVAFNSYGETVPAAAL
                     TWASSDPAAGTIDDDGVFTALAAGTTTVTAASGDIAGTAVVTVSSDEPVLSRIAITPP
                     AITLNAGDEETFVATAYDQHGTAMPADAVAWASSDPAAGTIDDGGLFIALDAGTTTIT
                     ATADGITGAADVTVVRPAPGVVVSPPAITLDEGETWLFTATLVDPEDDALFSGVVAWS
                     SHDETVGTIDEDGFFTALSEGAVTITATIDGSGEIGTAVVTVRPASAFPARIAISPSD
                     FTIAAGQDLRLTATVYDQYGDEMPGVAVAFESSDPHIGTIDDSGLFAALKDDSVVLTA
                     TAGDISGSARVTVEPSLAVPATIAIEPAKAVIAPGEIREFVATVFDQRDDGMDWVRVA
                     WSCSDAAIGTIDRAGLFTAYICGTADVTARAGGVEGIATVSVTTAPIPDDPGNNDESA
                     GWSGSSGGGDSGPAFDAGMGESLMRGETFTFSGITTSSVDSVAITAANSIPRLMVTVK
                     GTTAPSAAVPPDGDVYEYIEITLYWANPDDIESAVIVFTIPVDWLREHGMTPEDVGLM
                     RYVGGVWQPLDTVFLGEEDGAYRFRATTPGFSTFAIAAVAPENTTVTPEATATPGGEA
                     NTTINATAEPMATATTVPVTTAPAAPLVYAPFLAPLAFLLWRRRNH"
     gene            complement(18917..20362)
                     /locus_tag="PLI76_01785"
     CDS             complement(18917..20362)
                     /locus_tag="PLI76_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOI60791.1"
                     /translation="MTDDRINALPAVRRWLGNPVARALLRFIARDDECGNRLDNAIRY
                     YLDQNDGLCWRCRLAGHMVGLTLRRSSDLFGVSEDDIKKGLSETVFLRGLQNVLSGIA
                     RYGITMPQIVNAPFMVVWDFTHRCNLRCAHCYQDAQKALPHELDTDEAKRLVEELADA
                     GVVVIAFSGGEPLMRKDFFEVAAHAHKHDIYVALASNGTLITPEMAGRIKGAGVEYVE
                     ISVDGKDAASHDAMRGIPGAFDRTIAGVRNSVAAGFYTCIATTVTRANYAEVPDIYAL
                     ASDLGVNRLMCFNFIPTGRGVDMAGQDISPEERRDLMRYLMARTREGKGPEALTTAPQ
                     IAAVAVADEKGVPVGHFYAGEAIEGKTGLLADFIGGCGAGRLYCSIEPEGNVQPCVFM
                     PIVVGNVRDTPFLEIWHSAEVLQKLRDRSLLSGACADCGNKYICGGCRARGWAYFGDI
                     HAPDPGCINNRDYWESLLVGEVPVLPSQKKG"
     gene            complement(20472..21143)
                     /locus_tag="PLI76_01790"
     CDS             complement(20472..21143)
                     /locus_tag="PLI76_01790"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI60792.1"
                     /translation="MHPDSFFRKEILEDLRNRRGLLLKFVLPAGLLLPLILADLPVSL
                     QAAGITVAIIFSGIFGSSIGLVRIRESGMLDRMAMLPISPRRLIAGYILANALLDLAQ
                     IAIPLAVFLILHPPAPEVVFCVAGAFASAVVAANALGVLAAVAPGSSSEGHLYAIITV
                     MAAIVLSGLVAPAPHTQGLLPFWYLHAALVTAPGAFALSPVLAGLILGSALAVSPRLF
                     GRTRG"
     gene            complement(21133..22071)
                     /locus_tag="PLI76_01795"
     CDS             complement(21133..22071)
                     /locus_tag="PLI76_01795"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI60793.1"
                     /translation="MAVALRDVTKRFGSVRALDGVTLDVGKGEILGLLGPNGSGKSTL
                     LKVIARLIPPDEGTVLLPANGRAMMRRTGMLFDHTAHWEALTGYENARFFARSYGMEP
                     GAAHDRLESLFSLLDLRDRWDDPVSTYSYGMRRKLGLIEALAHEPSLILLDEPSIGLD
                     YRARVTLAGLLREAARGGAAIVFSTNDVNEAADLAHRVALFDRGRMLVAGEPSVLVGS
                     LGATVTIDLRLKAPIDPRPLGQVSGIEGVGTEEREDGFHVTVIAVPDASGHSSLLARV
                     VHAVANEGGVVVGVDLHAPGLPDVFLAYTKEGADAP"
     gene            complement(22081..22281)
                     /locus_tag="PLI76_01800"
     CDS             complement(22081..22281)
                     /locus_tag="PLI76_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60794.1"
                     /translation="MQVPDARVVVFTPTKRFAPDFHRHILQGRIVGQTIRPGDRILVY
                     EVAETVPDGAVRVTRSTHLEFR"
     gene            complement(22268..23482)
                     /locus_tag="PLI76_01805"
     CDS             complement(22268..23482)
                     /locus_tag="PLI76_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOI60795.1"
                     /translation="MMYQQIFVGVDGSPCSDMAGDAALALTAGGRGRCTGCHVYAARM
                     HRQRFEDMEPGLPGQYQEEERLDDLRATHDDLISGGMALISDAYLAPLAEKAAAQGIP
                     FEPVIAEGRGYVEVLRLIRERRPDLAVLGATGHGQTPEVPLGGLAERVLLYAGTGDLL
                     LVRRPWSLKNRPVVVGVDGSDASYAALECAASIAAAHDTPLEAVAVYDPFFHSAVFPV
                     IAGALPEEAQRRFNFPAQEQLHDEIIDRGLEHLYRRNLERGAALAQALGARVHTAVVA
                     GRVCPQVHHYAAARGAGLLVLGRYGLHREEESLIGSNTLNLARMSTTNVLVVAPRETP
                     VTVPDLPRDAIPWTPEAEQVLSRVPPFARKMARMAVEEHVRARGLSRVTEDAVRDVAR
                     RSGRGGGQDAGP"
     gene            complement(23566..25815)
                     /locus_tag="PLI76_01810"
     CDS             complement(23566..25815)
                     /locus_tag="PLI76_01810"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOI60796.1"
                     /translation="MTKVTLVIASLLIIALIAGVGAQQAGLSNASSTNESSNQTTQST
                     PGNVTDLTNAGNPLAATFQNNESLTNLAPIPSFTADIGLELIGENFTAPMMVTSPDDG
                     TGRLFVVDQIGVAKIVDVNGTELPEPFLDLRDNLVKLDPTYDERGLLSIAFHPNYRNN
                     GKVYAFYSAPLRSEAPEGWSCTNHISEFQVDPDNPNTVNMSSEKVLMYIDKPYENHNG
                     GVLAFGPADGYLYISVGDGGKANDVGNGHTPGIGNAQDLTKIYGKILRIDVDSTATGV
                     TIVQQNQTENQTGGASANRTENPPEPTWTTFAGSLYGIPPDNPFAENQPEILDSYAYK
                     TVPQEIYAYGFRNPAYMAFDASDGNALYVAMAGQNLFESVLIVLNGGNYGWNIREGTH
                     CFDPNAPTAPGESCNSTGLLGEPLIGPIFEGGHDLGIVIVGGDVYRGNTVVGLQGRYI
                     FGYWSDSRTVGNGTLLAATPPAGWAEGALPPTAAGLTPEDNAMWQAQIVNATAQNSDT
                     LGMFLRGFGEDADLDMYVLTSDVGGPDASTSTGKVWKIVPPTIVNPALGAGALEVTPT
                     VEAENQTENQTGTTAVGGGMGAGATPLSSLSSSDPAGNIMEVLALTETGIPFEETPAF
                     GNFTLFTGLLRSTGVAATLEGPGPYTVFAPTNDAFERMDPDIREPILNGSRDSKDIVL
                     YHIVNGSSTLADLKNVSTLRTLQGENLTINATGTAVRVENATIAIPDIMADNGVIQGI
                     DTVLIPPGA"
     gene            complement(26048..>26680)
                     /locus_tag="PLI76_01815"
     CDS             complement(26048..>26680)
                     /locus_tag="PLI76_01815"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOI60797.1"
                     /translation="AGNATTTAGNATTTNTTAGAGATPSSSLAVTPDHGQVSQIWDLL
                     EPPAGDRTVWELLQATETAIPFEETPVYGNFTIFIDLLQTTGVNQVLQGNDSYTVFAP
                     TDGAFVELPPDDMNALTKTTGNQSGAINRTAVALNHIVPGSYTESALRNETTLVTLAG
                     SNLTVNATGNETRVGNATIIIPDLVAKNGAIQGINRVLLPADTGGAANLS"
BASE COUNT         4908 a         8275 c         8581 g         4816 t
ORIGIN      
        1 cggcgggatg gatacaccgg agatacgggc ggtgcgggaa gtgcagagag ggtgagccgg
       61 ggcgcgaact ctcaccccca cccaaacccg tacgccagca ccgcgaccag gaccaccatg
      121 atcccgttcc ggagcgccgt cgagacgaac atgatctccg cccccgtccg caccccgaag
      181 atggcgatat aggaagaccc gagccagcgg atgctcctgg tcacgctggt gaggacgttc
      241 ccgacgagga gggtgacgat gatctgctgc caggtcatat ccccggcagc gagcagggcg
      301 gacgcgacgc tcgcacctgc gacgaaactc ccgaattggg ccgcgatgat ggcaaacccc
      361 tctgccggaa cgggaaagac cccccctgcg ccctgcatca tcacggcaag ccggtcgaag
      421 acgcccgcgt tgatgaggaa cgcgacgatg acgatggtgg ggacggtgat cttgaggagg
      481 cgcacgagga tcttttttga ggaggtgcac gcagtcccga gcgcctcgcg gagggtgacg
      541 gccgatacct ccgccggggc ggcgggggag cgcggcgcgg gggtgagggt catccggccg
      601 acgatcatga tgaccagcgt ctttacgagg ccggcaagca tcagcaggcc gaagtagatg
      661 agcccgggga tgccgaggag cgggacgtag accgggagga ggtagcgcca gtgcatgacg
      721 accgtcggaa acgagttcat ggtggcggca atgatcaact ccttttggct gatgatctcc
      781 tttgtgtggt agtccaggag catggcgttt gcggccggcg gggagacgaa ggccatgaga
      841 aaactggtgc cgcattcctc gctcaggcag gcaaacgacg tgagcggccg ggagagagag
      901 gcgatcttcc cggcgatatt cagggcgacg agcacctcgg cgatgaagac cccgatgatc
      961 atcatcggga ccatctcccc gagcaggtct gcggcgagca tcaccgtcgg aacgatactc
     1021 tcgtacatgg ctctcccgtc cggcaacggc gccacgaaga ccgcgcctct ccggagatat
     1081 gatgaagatc atctcgaaat ctaataaatg atctgttcca tgcttgaaaa ttccattaaa
     1141 tatgatataa cacaataatg gtagcactga gccgcaggaa ggcgccggcc ggcaacaacc
     1201 tatatccccc cgggcgaaaa agaggtcgtt atgccgacct gcagcgactg cgccctctac
     1261 acgcgaaaga ccgcgaccag cggggaatgc agcatcaacg gccctgttcc tgccgaccgg
     1321 gatgccgggc ggtgcccgtc acggacgttc cggccgcgag gatagtcccg ccataggctt
     1381 ccgaatgggt ggaacccctc tctggagcgg atgctctcta tacaagatgg ccgcccgggg
     1441 ccacacccga ggttgaaggg cggttaccat gcagcagaga accggagctc cggcgatgta
     1501 ttcggatcga ctctcctcaa ataatatgaa aattcgggca aataaataag attaagtatg
     1561 agcaccgata caagatttat tacttggggg tgggttgcgg acgagaactc ctctccgccc
     1621 ggcagacctc ttccagtatc gcagatacgg tgattgaagt atggatgtga ctcaagattc
     1681 ttctggacca ctggctgttg tcccccggtc tctctttcgg gattacagag agttcctgct
     1741 caccccgggc acgatcttca cgcttgttag cagtatcctc ctggcgatcg cggtcttata
     1801 ccaggtttcc ggcctcttta tcgacgcagc tccggaagcc tatgccgacc ggatatatct
     1861 cgcagctgct ctggtgggat cttcgttcat ctggtacagc gccgtccagg ggatccgcga
     1921 aggcgatttt accgctgata tcccggtctc tcttgccacg gcagcagcaa tcgcgatcgg
     1981 gcagtacccc gccgccgccg tcgtcgccgt actccttctt ctcggcgggt tgcttgaaga
     2041 actcgttgcc gcccgtgccg atcgggcgct tgatgcgctg gcccggttgc tgcccgaccg
     2101 ggtgaccgtc cggcgtgatg gtaaagatct cttcatttcc cctgacgagc tgatcgtcgg
     2161 tgatcttctc atcgtgcgtt ccggggaacg gatcgctgtc gatggggagg tcctgtttgg
     2221 agcggctgcc atcaaccagt cggcaatcac cggggagagc gtaccggcgg agaagcaggc
     2281 aggggatacc gtcttcgccg gcactctcaa cgagaacgga gcaatcgagg tccttgcaac
     2341 ccgggtcggg accgagacca cgctcggcca gatccgcagg cttgttgcgg atgcccagga
     2401 agagaaagcg cccgtcgaac gggtgctcga tcgctacgct aaactgtata cgccggcagc
     2461 cattatcctg ggattgctgc tgtggtggtg gagcgggaac gtcctgcagg cgatcacgat
     2521 gctcatcgtc ttctgcccgt gcgtaatcgt cctcgcgaca ccgacggcac tcgttgcctc
     2581 gatcggaaat gccgcgctca ggggaagtct tgtgaagaaa ggggcggccg tggagacgct
     2641 ggctcatgtc gatatcgtga tcttcgataa gacggggacg ctcacatccg gggagttgca
     2701 tctcgccgat gtggttcctt tcgacgatgt actcgaaacg agcctgttag gatacgctgc
     2761 atccgccgag aagttcagcg agcatccggt gggcagggcg atcgtgcggg ctgctgccgg
     2821 gcgggagatc gcggtcacgg atcctgagtc ctgtgaactg ttgacgggcc tcggcgtgaa
     2881 ggcccgaacc ggcgggcacg cggttctgct cgggcgcccg aaactcttct ccgatatggg
     2941 gatcaccctt cccaagaccg ttctttggga gatcaatgcc cgggcctgtg aaggaatgag
     3001 cgtcatcgtc gtgatgatcg accacgcggt cagcggtctc ctggtctttg aggatacgct
     3061 tcgggagcat gcgcaggaga ccgtcgccgc cctcaatcag gcaggcatca ggacggtcat
     3121 cgtaacgggc gacaaccggg agacagccgc ccggatcgcg gaagcgaccg ggatatccga
     3181 gatgcatgcc gagatgatgc cggaagagaa agtcgggatc gtgaagcggt ttcaggaggc
     3241 agggcaccgg gtggcatttg tcggcgatgg agtgaacgac ggtccggcgc ttgcaactgc
     3301 tgatgtgggc gtcgctatgg gcaatgccgg cacggatgtc gccatcgaga ctgcggatgt
     3361 cgtgctctta tcggatgatc tcctcaagct cccccggatc atcgatacat cgcgaaaagc
     3421 cctccagacc atccggcaga acctcgtttt tgccgtaggg gtcctcctct ttgccgtatg
     3481 cctcaccgtc ttcaacatcc tgacgccggt taccggggcg ctcctgcacg aactctcctc
     3541 gatcccggtg attgccaact cggcacggtt gattggaatg aagtgaactg tcttgcgcct
     3601 tttgggcggg gcatcccgct ccttcaaccc agcaggatga aattcgttcg ttcttaaggt
     3661 cacccggggg tgccgggcgc ctttctgcta ataggggcgc caggtggaat gcctcgcaaa
     3721 gagcgcggca ctatccacat ggcgtacaga cgatcggggt tcccggccgc atccgctcac
     3781 tccgggcagt cgatctcccg tgagtagggt ttgatatcga gaaccggcgt cccatcgagt
     3841 gcctccagcc ccctgacccg gatcgtgttg cccgttatat cgatgacatc gacgagcgag
     3901 aggctgatcg ggttcgggcg gttgggggaa cgggttgcaa acacgccgtg ctccaccgga
     3961 ttgtgcgggg gcgtcgcacg gagtgcggtc cggtcggccc gatcaaacca gcagagcacg
     4021 aagagatgtt tcttctcctc gaccctgaag agggcgggga ggtagtggtc gtcgatgacg
     4081 atcgtcgcct cggtgtcggt cagccggccc tggcgcgggg cgtccttctt ctccttgtat
     4141 ggcgaagata cgtgcccgat ggggttcaag tcgactatca tggtgtgcct ccggcagaag
     4201 cacgccggcc gggagcagtg cctgcctgcc cgtggagggc ggtggtctcc acggcccccg
     4261 gtgctacgaa aggccgtgcc aaaacggaag aggtgatatg tatcagtctc atgataggaa
     4321 tagttacgag aatttcctta agtctttcga aacgtgatgc accgccagga gatcctcccg
     4381 gcccgcgggg cgccgggtgt ggagaggtcc ggtgagcgcg gcccggggga tacctgtgga
     4441 atatggtgca tatgtgcccg tgatagtgcc gcttacccgg gtggaggggc acccggaacg
     4501 ggaaccgttt tctcctgccg gacgccaaca tcctgtatgg acgacgcgac ggccctgatg
     4561 ctcaaccacg tccgggcagc gctcccggca tcgggggccc acggttttga ccacaccctc
     4621 cgggtcgtgc gtcttgctga agcgatcggc gcccgcgaag gtgcggatat ggcggtcctc
     4681 ataccggccg caatcttcca cgatatcgcc cggcccgtcg aggaggagac gggcgccccc
     4741 cacgaggagg cgggggcgcg gatggcagaa gcctaccttt gctccatcag ctacccggcg
     4801 gaccttgtcc cgggcatcct ccacgccatc cgggcgcacc gctactcctc aggggtcgac
     4861 cccggaaccc tggaagcccg ggtgctctcg gatgcggaca acctggacgc gatgggcgcg
     4921 gtcggtatcg cacggacgtt catgcaggcc ggggagaagg gcggcggcat cctggacgca
     4981 accgatcaca tccgggcaaa actcgtcaac ctgaggggcc ggatgtacac ggataccgcc
     5041 cgggagattg ccgggaagag gcacgccctc ctcgttgcgt tcctcaatgc cctggatgac
     5101 gaggtgcggg gaccttaacc gccgtccctc tccacgggcc ggcgaagcag gaggcgtata
     5161 ttccggacga tcgtcttcct ctccgggaca tcctcgatga cgaggaggag cgagcctgcc
     5221 agcaggatgg cgataccgag cccgaggacc gatggcgcga gggtgacgag catcgccgcg
     5281 caggatgcgg cgccgagcag agggaagacg ggcccgaacg ggatgacaaa gcggttcttc
     5341 tccggtgaga ccagcgtttt gcgccctgtc aggatgagcg cgctgacgtt cacgagcatc
     5401 atcgccacga ggatcgtgac gttggtcagc tccacgatga acgagaggtt cccggcgagg
     5461 atgaggatgg cagtgatgcc ggcgccgagc aggatggagt tcgccggttg accgttccgg
     5521 agccgggcaa acctcgcggg gatctgttcc tcacttgcca tctccaggat gacccgcgag
     5581 gcgccgagga tgtttgcatt cgcggacgaa agcgtcgaga agagcgccgc gagagcgacg
     5641 acgaccccgc cgatgggtcc gaggtagacg accgccgcat cgaagacgct ctcgcccccg
     5701 tacgagggga gtcgcgccgc gagcagcgca acgacgaccc ccgtatatat cagcgccacg
     5761 atgccgatgg acgcaagggt ggcgagcggc acgttttttg ctgaatcctt cacctcgccg
     5821 cccatcatgg ccacgacctg gaatcctatg taggcgaaga agatcatcgc caccccccgg
     5881 agcatgccgc tcgcgccgcc ggggaggaac ggcgagaagt cggaggcctg aacgtgggtg
     5941 agcccgacca ggatcaggcc ggcgaggatg gccaccttcg cggcgacgag gccgatctcg
     6001 acccgccccg cctccgcggc acccctgagg ttgagcgccg caagcagcgc aaggccggca
     6061 agggcggcgt aacgcgggtc aaggctcgcc cctgtaagca ggtttgcgta gatcccgaac
     6121 cccaggagga cgaaggcggt ggcgatcgtt atgctgatgt acatagcaaa acccgcgaga
     6181 aacccgggat acggaccgag gatgcgccgt gcgtagaggt atccgccccc gtcctcgggg
     6241 tagatgctcg cgagcaccgc gtaggagagg cccgagaagg cgacggcaag accgcagatg
     6301 atgtaggaga tcaccgccga aggaccggcg atggcgatga tgaggccgct caggacaaag
     6361 accccggccc cgatcatgtt ccccaccgca aacgctaccg cggcagaaag gctcaaccct
     6421 ttcgaccgct ttgctccgcc cggcatcggg gtctatgtcc ggcaggcagg agataaggct
     6481 gccggcaggg gatttgcact gtgccgccca cgcacctttg ccggagatga ctgccgcctc
     6541 cgggcagaaa caagggcccg ggcattgcga gacggcgccc tctccggcac ttacgtatcc
     6601 gaaaagaacc gcagccccac gatccccgcc acgatcagga cgatgcagag aaggcggatg
     6661 acgctccgcg actcgccgaa gaggatgatc ccggcgatga ccgtcccgac cgccccgatg
     6721 cccgtccaga cggcgtacgc cgttccgagg gggagactcg taagcgaccg cgagaggagg
     6781 tagacgctgc ctcccatcac gataagggtc gccaccgacg gccccacctt cgtgaaccct
     6841 tcggtatacc tgaggccgag cgcccagccg gtttcgagga gcccggcgaa aaacagggtt
     6901 atccaggcaa tctcccgcat agatcctctc ctctttcgcc gggcaggcga tatactttgc
     6961 ccgaccccct tcccgatggg ccgtcacgtt taaggcccgg gagacccatt cccgaaatag
     7021 aggtagcgag aatggcaaaa gttaccatcg agagaccgga atgcatcagc tgcgagtcct
     7081 gctggacagt ctgcccggag atcttcgagc aggacccggc cgacggcctg agctcgatca
     7141 agaaagagta ccgggtcaac gacaaccccg cagagggcga ggtgcccgac gaccttatcg
     7201 actgcgcaat cgaggctgcc gatgcgtgcc cggtggcaat catcgaagtc gaagggtgag
     7261 acgatccggg cgcccgtgat gcgccgaacg aaaaaatagg ccttgaggag cggttacgcc
     7321 gatggcatga aaaagactat atcctttcag agctacgggg ggttttgcct gagagggaga
     7381 tcaaacccga aggcgcatca tcatggtgca acaggtttac ggatcagaac aatgagggtc
     7441 cgtaaacaaa aaaaacgttg caccaatgaa tgaaacgcaa ccgggacaag aatcaaactc
     7501 tcgggcacac gtattcttcc gcagtctgcc cggggagcgg acgccgcctc ttctgcacct
     7561 ccgttccggc taaaaaatta gaggggtttt cccatagcat cagagcgcgg gtttcccctt
     7621 cagcgcctcg acaaggagcc ggacgcccgc ctcgatatcg gcggtatcga ccacctcgac
     7681 cggcgagtgg atgtagcggg tcgcaatcga gaacggtatg ctcggtatgc cgccccgctc
     7741 caggtggatg atcgtcgcgt cggtgttgcc gccggtcccg acctcgagct ggcaggggat
     7801 gttgttcttc tccgcggtct ccctgagcca cgcggtcatc cggggatcgg ccatcagccc
     7861 gcgcccgctc gcgctgacga ggaccaggac cgggcctttt cccatctcga cgctcgcatc
     7921 gcgcttctcg atcccggggt ggtcgccggg gatggtgacg tcggtcgcta tcgcacagtc
     7981 ggggtttaag gtgtaggcgc tcaccttcgc tcctttgagc cccagctcct cctggaccgt
     8041 gaagacggcg tagaccgtgt ggggcgacgt catctgctgc agagcccgga tgagcatcgc
     8101 gacaccgacc cggttgtcga gcgcctttcc cgtgacgcgg ttgccggcaa gctccgtgta
     8161 ctcgcggtcg atggtgatcg gggtgccgat ctcgatgccg aggtccgcca catctcccgc
     8221 ggaggcagcg cccacgtcga tgaacatatc ctcgatcttg atctccttct tgcgctcctc
     8281 gtctttcatg acgtgcgggg gttttgcgcc gatgacgccc ttcaccggcc ccttcgtgcc
     8341 gtgcagaacc acccgctggc agtagagcac cggcccgaac catcccccga gggggacgac
     8401 ccggatgaac cctttctcgt cgatgtactg gaccatcagg ccgatctcgt ccatgtgggc
     8461 ggcgagcatg atggaaaagc cgtcgccgcg cttcgtggcg ataaggttcc ccatcgtgtc
     8521 ctcggtgatc tcgtcgacca caccggcaag ctccgccctg acgatatcgc ggatgttccc
     8581 ttccctgctc gtgacgccgt gagcgtccga taacttcctg agtaactctt tgaccatgct
     8641 caactcactc catcagcgcc ccgatcctcc cgaccgcccg cgagaggttc tcctgagcgg
     8701 tcgcgtagct gaagcgtgca tggtcgggcg cccgcgtgcc gaacgccgcg cccggcacga
     8761 tgatgacacc tgcttcgacg gcccgctgga taagggtctg gcccatcgga acaaacatgt
     8821 agaacgctcc ctccggccgg ggaaaatcaa acccgaggcc cgtaaggccc gcatacagga
     8881 ggtcccgccg cgcgcggtac tcctcccgca tccgcgcgac cgcgctctgg tcgccggtgt
     8941 aggcggcgag cgcggcatac tgcgcgatcg acgtcgcgca cgcctgggcg tactggtgta
     9001 tcttgagaca ctcgggtatg tagtcctccg gcgccgcaag gtagccgacc cgccagccgg
     9061 tcatggcgta ggtcttgctc gcggcgttga tggtgatgac gtcctcgccg aaacgggccg
     9121 cgctgacgtg tttcttctcg tagatgaagt gctcgtagac ctcgtcggag acgacggtga
     9181 caccccggtc gcccgcgtac tcgaccaggg cgcggaccga ctcctcgctc tcgacggcgc
     9241 ccgtcgggtt tcccggcgtg ttcaggacaa agagccgggc tccgtccatc tgctcctttg
     9301 cccgctcgac gtcgatatgc agggttgcat cgagcccgat accttcgggc cgcccgccgg
     9361 caaacgtcgc aagggcggca taggagacga acccggggtc cgcgaagagg acgcggtcgc
     9421 ccgggttcac gagcgcctgc atgacgaggt ggagcgcctc gctccccccc gccgtgacca
     9481 ggatctgctc cggccggtac acgagatcgt tttcgcgggc gaacttaccg cagatcgcct
     9541 cacggagttc ggggataccc gcgttcggcg tataccccgt ctttcccgcc ctgatggcgg
     9601 caatcgccgc ctccttgatg tggccgggcg tatcgaagtc cggctgcccg atgccgaggt
     9661 tgatcgcgtt cggccccccg gcgtcgaaga gtttccggat ccccgacatc tccaccgacc
     9721 ggacccggtc ggcatacctg tgcgtgttca taccgttcct cactcgatgt ttgcgtgccg
     9781 gcactggagg tcttcgggcc ggcactgggt gatctccatg agatgggata taatggcatc
     9841 tgcaaagacc atggcggtcg tctcgaatat ggtgccgagc ggtgcgaacg atttgtgttc
     9901 gcccatcatc tgccggatct cgaactccgc cgactcgtcg gccaccttat cccgctggct
     9961 ctcgatgacg acgacgcagc ttgcgatgcg cccgatgcgg gagtctttct ttgaggtgat
    10021 gaggcagacc ctcccaccga tctcctttgc agtctcggat atgtctgcaa ccgtctttgt
    10081 cgcgccggac ccggagaaga cgatgatgac gtctcccggc ttcatggccg gggtcacagt
    10141 ctccccgacg acgaacgccg agagcccgag gtgcatcaag cgcatggcga acgattttgc
    10201 aaccaggccg gaacgcccgg cgcccatggt gtagatgcgg tttgcattca agatctcgtt
    10261 taagagcgca tcgatctggt catcggacat cacgtccgcg atggtcctga tcttggtcgc
    10321 catcagccgc atcatattct taaccgggtg atctgtcatc gtagctcctc tgataggttt
    10381 cacgagatca tacattagaa tatcccagag gccccggtac ggggacgatt tccggggggc
    10441 gcaggggccc ggggaggggg gaggggcatt gatgaaaagt catacctgat agaatttttc
    10501 cccgcagcag gaggcgggcg gtttccaggg ccggaggatc gcaattatat cgcttgaatg
    10561 gccgaaatgc cggcataaaa cctgcatctc ccgcctgatc cgtctcccgt aaccgcgcct
    10621 tccggcgaaa aggctatcat cgatgattga gaaatataca aagtccagca cggcaccctg
    10681 atcataacgg gagcagaaat actgcatgat tccattttca tggtagtatt ctctgattac
    10741 gtctcccctg ggtgccgatg tcgataaata tccctccatc tggagcggat cggtgatagc
    10801 gcgggttcgc cgccccctgc atcgttatgc gggagggcgc ggggcccggg ggataccagc
    10861 gggtgagaga agatggcagg gcaggaaggg cgttcggacg aagagatcgc cgatctcctc
    10921 gatgagaacg tgcgggaatg gtgcaggcga cagctcgacg ggcggttcac gccgccgcag
    10981 aggatggcgg tcccgctcat ccacgataac cggaacgtcc tcatatgctc cccgaccggg
    11041 tcaggaaaga ccctcgcggc gttccttgct atcatcgacg acctcgtggt ccgggcacga
    11101 gccggagaac tcgaggatac cgtatattgc ctctatatat cgccgctcaa gtcgcttgca
    11161 aacgatatcc acaagaacct cagcctgccg cttcagggaa tagaggcgat cgcacgggag
    11221 cggggggtcc ggcacacccc tatccggcac gccgtccgcc acggtgacac ccccaaggca
    11281 gaaaaggcga agatggcgcg aaaaccgccg catatactca acatcacccc cgagacgctc
    11341 ggtatcctcc tgaactcacc gaagttccgc gagagcctcc ggacggtccg gtgggtcgtc
    11401 atcgacgaga tccactccct cgcgggttcg aagcgcggcg tccacctctc ggtgagcctt
    11461 gagcgcctcg aagacctggt gaaggaggcc ggcggcggat ttacccggat agggtgctcg
    11521 gcgacgatcg aacccctcga cgaggtcggc cggttcctgg tcggcgtcgg gcgcgaggtc
    11581 tcgatcgtcg atacccggtt tgcccgggag ttcgacctct ccctcatctg cccggtcccc
    11641 gaccttgccg acaccacccc cgaagacctc tcgcggcacc tttactcgac gatccaccgc
    11701 ctcgtcgggg agcacgagaa cacgctcata ttcacgaaca cccggaacgg cgccgagcgg
    11761 atcctccata acctccgggc ccgctacccg gagtattaca ccgaagagaa cacggggtgc
    11821 caccacggtt cgatgggggc ggaagggagg cttgccgtcg aggagaaact caaggccggg
    11881 aaggtgaagg cggtgacgac gtcgacgtcg cttgagctcg gtgtcgatat gccccacgtc
    11941 gacctcgtcc tccaggtcgg gtccccgaag tccgtggcgg cgctcctcca gcggataggg
    12001 cgcgccgggc accgcctcgg ggaggtggtg aaagggcgga tcgtcgtcct cgaccgcgac
    12061 gagctcgtcg agtgcgcggt gatgctccgc gaggggcaga gggggtttgt ggaccgcatc
    12121 cacataccgg agaactgcct cgacgtcctc gcgcagcacg tctttggtat ggcgctcgat
    12181 gggagccggc ggatcgatga gatgcttgcg gtcttccgcc ggtcccactg ctacagggac
    12241 cttgaggagg ccgacctcgc aagcgtcgtc cgctaccttg cgggagagta tgcggggctc
    12301 gaggagcggc acatatacgc gaaaatctgg cacgatgcgg cgaccgggac ggttcggaag
    12361 cgggggcgaa acgcccgtat gatctatttc ttaaactccg ggaccatacc ggacgagttc
    12421 tcctgcgacg tcctcacccg tgacggtgtg tttgccggca acctggacga gaagtatctc
    12481 gagcggatga acaagggcga cgtcttcgtc ctcgggggcc ggcgctaccg gtttgcctac
    12541 cgccggggcg gaaaactcta cgtcgacccg accggcgacc gccccacgat accgagctgg
    12601 ttctcggaga aactcccgct ctcgttcgac ctcggtctcc acgccctccg gttcaaggag
    12661 agtatgcttc gcgcgatgga ggcgatgggg acggaggacc tcgtcgatgc gctcctctgc
    12721 gagtatccga tcgacgagaa cagcgcccgg agcatatgcg cgatcttcga gcagcaggtc
    12781 tgttatctcg gagaggaggc cgtccccacg ccgggccgga tcgtcgtcga ggagcaggtc
    12841 gaccgcgaga accgccgccg cctctacttc ttcatgacca actacgggct gcggttcaac
    12901 gacggattct cccgtatcgt cgccttcctg attgcgcggg acgtgacgac gaacgtctcg
    12961 gtcggtatca gcgatacggg gtttctggta tcgataccgc ttgagaagcg ggccgacccc
    13021 atgcgcctgc tccggggtat ccgggcgggc gagtgccggg atatactcaa agaggccgtc
    13081 ggagggacgc agcttgagaa acgggtcttc cggatcaacg cggcccggtc gtacatgatc
    13141 ctcagaaact acagcggcag aaagaggagc gcccgccgcc agcaggtgag cgccgatatg
    13201 ctgatatcgt ttgcaaaacg ccttcccggg tttgccgtga tgcgggagac ctaccgcgag
    13261 gtgatcgaag accggttcga ggtggcaaac atcgagaaga tcgtcgggga gatcggcgcc
    13321 ggcgagatcg atatcgccct gacccggacg gcgtcgccca gcccgctcgc gttcggtata
    13381 gcgacgctcg gcatctccga cgcgatatac gcggaggacc ggctctcgat cctccgcgag
    13441 ttccagaggc gggtgatgag cagcatcggg agcggggcgg cggcatgagc gcggtcagcg
    13501 aggccgacct cctctcccgg ttcgggttct acaggagcgc tctctacatc cgggatatcg
    13561 acacctgcgt cgcctccgat atccacatcg ggcttgccga gacgctctac cggcaggggc
    13621 tccacttccc gcttcacgaa gaggagacgc tgatcgagcg gttcgaggcg atactcgacc
    13681 ggttcaaccc ggcggtcttc gtcttcgacg gggatatatt ccacgccttc gaccgggtga
    13741 gtcggagcgt cgtggagacg ttcgaggcga tccttgcgac cctccgctcg gagtgcgagg
    13801 tcgtcctcgt cacgggatcg cacgacacga tgctccctgc ggttgcgggg gggacggtcg
    13861 agcgctacga ccgcggcggc tacacggtgg tccacggcga ccggccggtc ggagaccacg
    13921 ggacgctcgt catcggccac gatcacccgg cgatcgagat cgatatggcc cggttcccct
    13981 gtttcctcta tggggagggt gttttcgggg ggaaagacct cgtcgtgatg ccggcgtata
    14041 acccgctctc ccccggcgtc acgatcaacc acatgcagcg ccgggacttc ctctcgccca
    14101 tcctccgccg cgtagatgca gggagcctcc agccggtggt ggaggtcgac ggcgagacgg
    14161 tggtcctccc gccgcttgcg ggcatccggc gatttgcgtg aggcggttct ccggcatcca
    14221 tagggggcta taccggatga ggggaggacc tggatcccgg agggatgatc cgggttgcaa
    14281 acgtcgccgg caggggggcg ctttcacgcc ggccccgacg atggttgccg ctatctccga
    14341 ccccgtgata agatagccct tctctattcg tcaacgctgc aatctcgcga tgggtggcca
    14401 ttgagggcgc cctgattcca cctccgggat aagggtgagg aggcggatga ggtcaacgac
    14461 taccgttaac taccgtaaca gcgaaaagcg ttacggtaga tgcgctaccg tatcattttt
    14521 cgatgattcg atacgataaa agcggcccca gaattaaagg ccaatagtaa agtaaaggca
    14581 tgcccttgtc tggtttcgcc ccctccccgg ggtacccggt gatacggtaa accgttcacc
    14641 cttatgggag ggacttttgg gatcaggata agcccggatt atccctgcta tacttctgcc
    14701 gatatgttgt gctgatacgg gtagcgctcc ggtatgggtg cagggagtga cctcagcccg
    14761 tgtactcccg cctccccggg tgcgtgccgg gcgatacacc ttccggccga tcgcagggga
    14821 ttcatgggga aagttgtcgt ttgggggctt tgccggaggc aacggtnnnn nnnnnnnnnn
    14881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14941 nnnnnnnnnn nnnnnnnnnn nnnnnncgca ggggattcat ggggaaagtt gtcgtttggg
    15001 ggctttgccg gaggcaacgg ttcacggtgc tcctcgatct gaaccatcct ccccggccgg
    15061 ctgaggggac ccgtcccccg actcctctcc ccccgggagc gcacaggaaa tttcagttta
    15121 taatataata attttggaat aaatcaccgt aaaagatata tatttatgct gctattcacc
    15181 ctttcaccgg ctaccttatg accggacttt aaataggatt tcgcactccg tctgccgggt
    15241 gcccaatgga gcacctgctt tagcgagccc ttcgtgcata ccggagggta tgcaccgcgg
    15301 tctttgtggg cgggggtgga gaagagacaa tggagaggaa tatgacaaag gttacacacc
    15361 tggcattatt gtgcgcgttt gtcgccatcg ggctgctcat atcgccggta atggctgtag
    15421 aggctgcgtc gggggagcat atctatcgct acctcgacca tgcgcaggat caccccgatg
    15481 tcgacggcaa catgatcgtc tgggaagatg atagggacgg agacaaggac atctacttcg
    15541 gtacgataga cgggttccgg gaatcgcccg gctataccgg cgagaggata acggccgatc
    15601 ctgcgaggca ggagaggccg tccatatcgg gcaactacat cgtctggcag gataaccggc
    15661 acggaaaccc tgatatctac ctctacgatc gcctcaacgc gaccgagacg cagctcacca
    15721 cctgcacagg caggcagtgg ttgcctgcaa tctccgggaa ctacgttgcc tggtacgacg
    15781 atagcagcgg caggacaacc atcgtcctct acgatatcgc cggcaagacg gtgaaggacg
    15841 tcatcgattg cgatgcacgg acgacgatcc ccggcgcatc gatcgagttc aagcccgcac
    15901 tctcggagaa gtatgtcgcc tgggtggaag acgaagatgc gagggtccga tactacgaca
    15961 ttgcggccgg cacaatcgca ggcacggtct ccacgagcat ggcggtccag tgctggccct
    16021 cgctctccgg gagcctcctg gtctgggaag attaccggga cggcaacgcc gagatctaca
    16081 tgttcgatct cgataacccg tccggcaatg agcagcagat cacctccgac gcctccgatc
    16141 aggtcgcgcc ggcgatcagc ggcaggatca tcgcctggga ggacaatcgg gataaggggc
    16201 cggatgaaac ggatggacgc ggcatctacc tgtacgatct tgcatccggg aaagagctgt
    16261 cggtaaagct accggatgag aactacgacg agcacttcta ccctgccgtc agtggcaaca
    16321 ctgttgtctg gcagcggggc gcgaccccta actcaaacct ctacatcttc gtctacgaac
    16381 cggacgtgcc ggtcgacccg gccctgacgg acatcacggt cacgccatcc tcggccacgc
    16441 tcatggtcgg cgagacgaaa gggttcgtgg caaccgctct tgaccaggac ggcaacgcca
    16501 tgcccggcat cgatatcgcc tggtcgagca gcgacccaac cgtcgggacg atcgatgatg
    16561 acggcgtctt taccgccctc gttgcaggta cggcgaccgt cactgcggca tcaggcgata
    16621 tagccggcac cgcgaccgtc accgtcagcg acgaagaacc ggtgctcacg ggcctgaacg
    16681 tcacgcctcc ccgggccacc ctcgcagccg gcgatagcct gaagttcatc gtgaccgccc
    16741 gcgaccagaa cggcaacgtc atgcctgcgg gtgctgtcgc ctggacgagc agcgatccgg
    16801 ccgccgggac gatcgatgat gacggcgtct ttaccgccct tgccgcgggt acggcgaccg
    16861 tcactgcgac atcgggcgag gtcgccgcaa cggcggccgt caccgtcatc gctgcagaac
    16921 cggagtttgg tatggttgtt ctctcgccgc cggaggtcac tctcgagacc ggcgacaccc
    16981 tgcagtttga ggccgtcgcg ttcaactcct acggcgagac cgtgccggcc gccgccctca
    17041 cctgggcgag cagcgatccg gccgccggga cgatcgatga tgacggcgtc tttaccgccc
    17101 ttgccgcggg tacgacgacc gtcactgcgg catcaggcga tatagccggc accgcggttg
    17161 tcacggtatc ctccgatgag ccggtcctct cacggatcgc catcacgccc ccggcaatca
    17221 ccctgaacgc cggtgatgag gagacgttcg tcgcaaccgc ctacgaccag cacggcaccg
    17281 ccatgcctgc ggatgctgtc gcctgggcga gcagcgatcc ggccgccggc accatcgatg
    17341 acggcgggct cttcatcgcc cttgatgcgg gcacgacgac catcaccgca acggccgacg
    17401 gcatcactgg agcggcggac gtaacggtcg tccggccggc cccgggcgtc gtcgtctccc
    17461 cgccggcgat caccctggac gagggagaga cctggctctt caccgcgact ctcgtcgatc
    17521 cggaggacga cgctctattc tccggagtgg tcgcctggtc gtcgcacgac gagaccgtcg
    17581 gcacgatcga cgaggacggg ttctttaccg cgctcagtga gggcgccgta accatcaccg
    17641 cgacgatcga cggatccgga gagatcggga ccgcggtcgt gacggtccgg ccggcatcgg
    17701 cgttcccggc gcgaatcgcg atcagcccgt ctgacttcac tatcgccgcc gggcaggacc
    17761 tgaggctcac cgcgactgtg tatgatcagt acggcgacga gatgccgggc gtcgcggtgg
    17821 cctttgagag cagcgacccg catatcggca ccatcgatga ctccggcctc tttgccgccc
    17881 tcaaggacga cagtgtggtc ctcactgcaa cggcaggcga tatatccggc tccgcccgcg
    17941 tgaccgttga accctccctt gcagtcccgg ccaccatcgc aatcgagcct gcgaaggccg
    18001 tgattgcgcc cggggagatc cgggagttcg tcgccacggt ctttgaccag cgtgacgacg
    18061 ggatggactg ggtcagggtc gcctggtcgt gctctgatgc agccatcggc accatcgaca
    18121 gggccggcct ctttacagca tacatatgcg ggacggcgga cgtgacggcc cgggccggcg
    18181 gcgtggaggg aatcgcgacg gtcagcgtca cgaccgcacc gatccctgac gatcccggaa
    18241 acaacgacga atcggccggt tggagcggta gcagcggcgg cggggacagc ggtcccgcct
    18301 ttgacgcagg catgggcgag agcctgatgc gcggcgagac gttcacgttc tcgggcatca
    18361 ccacatcatc ggtcgacagc gtcgccatca cggcggcaaa cagcatcccg aggttgatgg
    18421 tgaccgtgaa agggaccaca gcgccctctg cggcggtgcc ccccgatggt gacgtctacg
    18481 agtacatcga gatcaccctc tactgggcaa accctgacga catcgagagc gcggtgatcg
    18541 tcttcaccat ccccgtcgac tggctccgcg agcacggcat gacaccggag gatgtcgggc
    18601 tcatgcgcta tgtcggcggc gtctggcagc ccctggatac cgtcttcctc ggtgaggaag
    18661 acggggcgta ccgcttccgc gcgaccaccc ccgggttctc cacgtttgcc atcgctgccg
    18721 tggcgccgga gaacacgacc gtaacgccgg aggcgaccgc cacgccggga ggggaggcga
    18781 acaccacgat aaacgcgact gcggaaccga tggcaacggc gaccaccgtg ccggtgacga
    18841 cggcgcccgc ggcaccgctc gtctacgcac ccttccttgc cccgctcgcc ttcctcctct
    18901 ggcggagaag gaaccactaa cccttttttt gggacgggag aacgggcacc tcgcccacga
    18961 gaagcgactc ccagtagtcg cggttattga tgcacccggg gtccggcgcg tggatatccc
    19021 cgaagtatgc ccacccgcgg gccctgcacc cgccgcatat gtacttgttc ccgcaatcgg
    19081 cgcatgcccc ggagagcagg ctcctgtccc gcaacttctg cagcacctcc gctgagtgcc
    19141 atatttcaag gaacggcgta tcccggacgt ttccgacgac gatcggcatg aagacgcagg
    19201 gttggacgtt tccttcgggc tcgatgctgc agtagagcct ccctgcgccg cacccgccga
    19261 tgaagtcggc aagaagcccg gtcttcccct cgatcgcctc cccggcgtaa aagtgcccga
    19321 cgggaacccc tttctcatcg gcaaccgcca cggccgcgat ctgcggggcg gtggtgagcg
    19381 cctcaggacc ctttccctcc cgtgtccggg ccatcaggta gcgcataaga tctctgcgct
    19441 cttccgggga tatatcctgg ccggccatat ccacgcccct ccccgtcggt atgaagttga
    19501 agcacatgag ccggttcaca ccgaggtccg atgcaagggc gtagatatcc gggacctcgg
    19561 cgtagttcgc ccgggtgacc gtggttgcga tgcaggtgta gaaccccgcc gcgacggagt
    19621 tcctgacgcc tgcgatggtc cggtcgaacg cgcccgggat gccccgcatg gcatcgtggc
    19681 tcgctgcatc cttcccatcc acggatatct ccacgtactc gacgccggca cccttgatcc
    19741 gccccgccat ctccggtgtg atgagggtgc cgtttgaggc gagcgccacg tatatgtcgt
    19801 gtttgtgggc atgggcggcc acttcgaaga agtcctttcg catgagaggc tctccgcccg
    19861 aaaaggcgat gaccaccacg ccggcatccg caagttcctc gacgagacgt ttcgcctcat
    19921 cggtatcgag ttcgtggggg agggctttct gcgcgtcctg gtagcagtgg gcgcagcgaa
    19981 ggttgcacct gtgggtgaag tcccagacca ccatgaaggg ggcgttcacg atctggggca
    20041 tggtgatgcc gtagcgggcg ataccggaga gcacgttctg gagcccccgt aaaaatacgg
    20101 tctcggagag acctttcttg atgtcatcct cgctcacccc gaagaggtcg ctgctccggc
    20161 ggagggtgag ccccaccata tggcccgcga gccgacacct ccagcataac ccgtcgttct
    20221 ggtcaaggta atatcgaatc gcgttatcga ggcgatttcc acactcatca tcccgggcga
    20281 tgaaccggag gagggcacgg gcaaccgggt ttccgagcca ccgccggacc gccggaagcg
    20341 cattaatacg gtcatctgtc attgcctgct catctctcct gcacccgggg atggcattat
    20401 ccgggggtga tccggatcac cccggagggc tgagatggct taaaggttgc ccgccgaaac
    20461 ctccgatgct ctcaccctcg agttctgccg aacagccgcg gcgagaccgc gagcgcggag
    20521 ccgaggatga ggccggcaag gaccggagag agggcgaacg ccccgggggc ggtgacgagt
    20581 gctgcatgaa ggtaccagaa cgggaggagc ccctgcgtat gcggggcggg cgcgacgagt
    20641 ccggagagga cgatcgctgc catgaccgtg atgatggcgt agaggtgccc ctcgctggac
    20701 gagcccgggg cgaccgccgc caggacgccg agggcgtttg ccgcaaccac ggcggaggcg
    20761 aatgctccgg ccacgcagaa aacgacttcc ggggccgggg gatgcaggat caggaagacg
    20821 gcgagaggga tggctatctg tgccagatcg aggagagcgt ttgcgagtat gtatccggcg
    20881 atcagccgac ggggggagat gggcagcatg gccatccggt cgagcatgcc gctctcgcgg
    20941 atgcggacga gcccgatcga cgacccgaat atgccggaga agatgatggc gaccgtgatg
    21001 ccggcggcct gcagggagac gggcagatcg gcgagtatga gggggagcag gagccccgcc
    21061 ggcaggacga acttgaggag gagcccccgg cggttccgga ggtcttcaag gatctccttt
    21121 ctgaagaacg agtcagggtg catccgctcc ctccttcgtg tacgcgagga agacatccgg
    21181 gagccccggg gcatggagat cgacgcccac gaccaccccg ccctcgttcg ccaccgcgtg
    21241 gacgacccgg gcgaggaggg atgagtgccc ggatgcatcc ggcacggcta taaccgtcac
    21301 gtgaaacccg tcctcccgct cctccgtgcc cacgccctcg atgccggaaa cctgcccgag
    21361 cggccgggga tcgatcggcg ccttcagccg gaggtcgatc gtcaccgtag cgccgagcga
    21421 tccgacgagc acggacggtt ctcccgcgac gagcatcctg ccccggtcaa agagggcgac
    21481 ccggtgggca agatcggccg cctcgttcac gtcgtttgtg gagaagacga tcgcagcacc
    21541 cccccgggcc gcctcgcgga ggagaccggc aagggtgacc cgcgcccggt aatccaggcc
    21601 gatcgagggc tcgtccagca ggatgaggga gggttcgtgc gccagggcct cgataaggcc
    21661 gagttttcgc cgcatcccat acgagtaggt agagaccggg tcatcccatc gatcccggag
    21721 gtcgagaagc gagaagagcg actcgagccg gtcgtgcgcc gcgcctggtt ccatgccgta
    21781 ggagcgggcg aagaaccggg cgttctcgta gccggtgagc gcctcccagt gggcggtatg
    21841 gtcaaagagc ataccggttc ggcgcatcat cgcccggccg tttgccggga gcagaacagt
    21901 cccctcgtcg ggggggatga gccgggcgat gactttgagc agggtggact tccccgaccc
    21961 gttggggccg aggaggccga ggatctcccc cttccccaca tcgagggtga caccgtcgag
    22021 cgcccggacg gacccgaacc gtttcgtcac gtcccgcagg gcgacagcca tccctccccc
    22081 tcaccggaac tcaaggtgcg tggaccgggt aacccgcacc gctccatcgg gcacggtctc
    22141 tgcgacctca tagacgagga tccggtcgcc cgggcggatg gtctgcccca cgatccgtcc
    22201 ctgcaggata tgccggtgaa agtccggggc aaacctcttt gtcggggtaa agaccaccac
    22261 ccgggcatca gggacctgca tcctgaccac ctcccctgcc ggaccgccgg gccacgtccc
    22321 ggacggcatc ctccgtgacc cgggagaggc cccgggcccg gacatgctct tcgaccgcca
    22381 tccgtgccat cttccgcgca aacggcggca cccgcgagag gacctgctcc gcctccggcg
    22441 tccagggtat tgcgtcgcgg ggcaggtccg gcaccgtgac cggggtctct ctcggcgcga
    22501 cgacgaggac gttcgtggtg ctcatccggg caaggttcag ggtgttcgac cctatcagcg
    22561 actcctcttc ccggtgcagc ccgtagcgcc cgaggacgag gagccccgcc cctctggccg
    22621 cggcgtagtg gtgcacctgc gggcagaccc tgccggcgac gaccgccgtg tggacccggg
    22681 cgcccagggc ttgcgcgagg gccgccccgc gttccaggtt tcgccggtag aggtgttcaa
    22741 gcccccggtc gatgatctcg tcgtggagtt gctcctgggc cgggaagttg aaccggcgct
    22801 gcgcctcctc cggcagggcg ccggcgatga cgggaaagac tgcggaatga aagaacggat
    22861 cgtagacggc caccgcctcg agcggcgtat catgagccgc ggcgatagaa gccgcacatt
    22921 cgagggctgc gtagctcgcg tcgctcccgt ccacgcccac gacgaccggg cggttcttca
    22981 ggctccaggg cctccggacc agaaggaggt cgcccgtgcc ggcatacagg agaacccgct
    23041 cggcaaggcc cccgaggggc acctccggcg tctgcccgtg cccggtcgcg ccgagcaccg
    23101 caagatccgg ccggcgctcc cggatgaggc ggaggacctc gacgtatccc ctcccttcgg
    23161 ctatgaccgg ttcgaagggg ataccctgtg cggcggcctt ctccgccagc ggcgcgaggt
    23221 aggcatccga tatgagcgcc atgcccccgg atatcaggtc gtcgtgcgtc gcccgcaggt
    23281 catcgagccg ttcttcttcc tggtactgcc cgggaaggcc gggctccata tcctcaaagc
    23341 gctgccggtg catccgggcg gcatacacgt ggcagccggt gcacctgccc ctgccgccgg
    23401 cagtaagggc gagagctgca tcccccgcca tgtcggagca gggagagccg tccaccccta
    23461 cgaatatctg ctggtacatc atgcacaccc gtcatcctgg atgacaggga taagagcggc
    23521 acaaacggca taaatgcttt ggatgatatc ttgcagaagt cccggtcagg caccgggcgg
    23581 gatgaggacg gtatcgatac cctggatgac gccgttgtcg gccatgatat cggggatcgc
    23641 gatcgtggcg ttctccaccc tgacggccgt tccggtagcg tttatggtga ggttttcgcc
    23701 ctgcagggtc cggagcgtcg atacgttctt gagatcggcg agcgtggagg aaccgttcac
    23761 gatgtggtag agcacgatgt ccttactgtc gcgggaaccg ttcaggatcg gttcgcggat
    23821 atcggggtcc atccgctcga atgcatcatt ggtcggggcg aacaccgtgt atggtcccgg
    23881 gccctcaagc gtggcggcga cgccggtcga ccggaggagg cctgtgaaca gggtgaagtt
    23941 cccgaacgcg ggggtctcct cgaaggggat gccggtctcg gtcaatgcaa ggacttccat
    24001 gatgtttccg gcggggtccg atgaagagag ggaggagagg ggtgttgcac ccgctcccat
    24061 tcctcctcct acagccgtgg tgccggtctg gttttcggtc tggttctctg cctccacagt
    24121 cggcgtgacc tcgagcgccc ccgcacccag ggccggattg acgatggtgg gcggcacgat
    24181 cttccagacc ttgccggtgc tggtagaggc atcagggcct cccacgtcgc ttgtcagcac
    24241 gtacatgtcc aggtcggcat cctcgccaaa cccgcggagg aacatgccca gggtatcgct
    24301 gttttgtgcg gtggcgttca ctatctgggc ctgccacatg gcgttatctt ccggcgtcag
    24361 acctgcggcg gtcggcggca gtgcgccctc ggcccaccct gccggcggcg tggcggcaag
    24421 gagggtgccg tttcctaccg tccggctgtc gctccagtag ccgaatatgt acctgccctg
    24481 gagcccgacg acagtgtttc cgcggtagac atcgccgccg acgatcacga tgccgagatc
    24541 atggccgccc tcaaagatgg ggccgatcag gggttctccc agaaggccgg tgctgttgca
    24601 ggactctccc ggggccgtgg gggcgttcgg gtcgaagcag tgcgtccctt cgcggatgtt
    24661 ccagccgtag ttcccgccgt tcaggacaat taagacggac tcaaagaggt tctggcctgc
    24721 catggcgacg tagagcgcgt tgccgtcgct ggcgtcaaaa gccatgtagg cggggttccg
    24781 gaatccgtag gcgtatatct cctgtggaac tgtcttgtaa gcgtaggagt cgaggatctc
    24841 gggctgattc tcggcgaacg ggttgtcggg cggtatgccg tagaggctgc cggcaaacgt
    24901 cgtccacgtc ggttcgggcg ggttctccgt ccggttggcg ctcgcgcccc cggtctggtt
    24961 ctcggtctgg ttctgctgaa ctatcgtgac cccggtggcg gtgctgtcca catcgatccg
    25021 gaggatcttg ccgtagatct tcgtgagatc ctgggcgttc ccgataccgg gggtatgccc
    25081 gttcccgaca tcgtttgcct tcccgccgtc accgaccgat atgtagaggt agccgtcggc
    25141 ggggccgaag gcgagcacgc cgccgttgtg gttctcgtaa ggtttgtcga tgtacatcag
    25201 gactttctcg gacgacatgt tcacggtgtt cggattatcg ggatcgacct ggaactcgga
    25261 tatgtgattc gtgcaactcc agccctcggg agcctccgac cgtaagggcg cgctgtagaa
    25321 agcgtagacc ttgccgttgt tcctgtaatt ggggtggaac gctatggaga gcaggccgcg
    25381 ttcatcgtag gtcgggtcga gtttcacgag gttatcccgg aggtccagga acggttcggg
    25441 gagttcggtc ccgtttacat cgacgatctt tgcgaccccg atctggtcca cgacaaagag
    25501 ccgcccggtc ccgtcgtcgg gcgacgtgac catcatgggc gcggtgaagt tctcaccgat
    25561 gagctcaagg ccgatatcgg ccgtaaaact cgggatgggt gccaggttcg tcagggattc
    25621 gttgttctga aacgtcgcgg caagcgggtt gccggcgttc gtgaggtccg ttacgttgcc
    25681 gggcgtgctc tgcgtcgtct ggttggaact ctcgttggta ctgcttgcat tgctcaatcc
    25741 cgcctgttgt gcacccacgc ccgcaatgag tgcgatgatg aggagggatg ctatgacaag
    25801 ggtaactttt gtcatgcggg tccggacatg gcacgagaat atataatggt tgtgccggat
    25861 cgtctgacgt tatccggggc atagagggtc aggattgaga gaacacggtt tttttgccgg
    25921 tatcccgcac catcgggtga tctgccgtca tccctgcggt gcgaccaaag accgcccgcg
    25981 aggtcatacg accccgcccc cctcccgcgc cggcgtttgt accgccctga aggatctttc
    26041 ggcgcggtca ggagaggttt gctgccccgc ccgtgtcggc cgggagcagc acccggttga
    26101 taccctggat ggctccgttc tttgcgacga gatcggggat gatgatcgtg gcgttgccaa
    26161 cccgggtctc gttgccggtt gcattcacgg tgaggttgct tcccgcgagg gtgacgagcg
    26221 tggtctcgtt acgaagggcg ctctctgtat acgatcccgg cacgatatgg ttcagggcga
    26281 ctgcggtgcg gtttattgct cccgactggt tgcccgtcgt cttggtcagg gcgttcatat
    26341 cgtcgggagg aagctctacg aatgccccat cggtcggggc aaagacggtg tacgaatcgt
    26401 tgccctggag gacctggttc acccccgtcg tctggaggag atcgatgaag atcgtgaagt
    26461 tgccgtagac gggtgtctct tcaaagggga tggccgtctc ggtcgcctgg agaagctccc
    26521 agacagttcg atcgccggcg ggcggctcca ggagatccca tatctggctg acctggccat
    26581 ggtcgggcgt cacggccaaa gaggaagagg gggtggcccc cgctcccgct gtcgtgttgg
    26641 tcgtggtcgc gtttccagcc gttgtggtcg catttccagc
//
LOCUS       DAOSEE010000012        25728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_867_length_25728_cov_259.211623, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000012 DAOSEE010000000
VERSION     DAOSEE010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 25728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25728
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_867_length_25728_cov_259.211623"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="PLI76_01820"
     CDS             complement(<1..501)
                     /locus_tag="PLI76_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60798.1"
                     /translation="MTMKFAEAIRKWTGWCPNAVGTVRRRYPAPDGEIGIGAVAGGSR
                     EAVEGVFVDYTSPRFLPVSILVFGGLFVAGLLIPSLRSVFLILTGLSFIAYAAVHLHL
                     DTKRAEIKSFEDVVVVQRSRSRPLTFGKDAFRSVEVKGPYPPGLRWMFALLPILLVAG
                     VYLARVW"
     gene            complement(498..977)
                     /locus_tag="PLI76_01825"
     CDS             complement(498..977)
                     /locus_tag="PLI76_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60799.1"
                     /translation="MTVFLEYIRKKLGWCPNAGAVRPVRRTSAEAGYGDAWKGKSPDP
                     NPGPEPAGIAGGDRGGYQENMLLILIALAWLLPVVYDREFLPILIILSAVAVYYDAQN
                     IRAGDKFEKETLLGDIVTWRPLTWGAATLVGGIIIMAIYLFHRKEIYRANFRGEASA"
     gene            complement(974..1711)
                     /locus_tag="PLI76_01830"
     CDS             complement(974..1711)
                     /locus_tag="PLI76_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60800.1"
                     /translation="MALRFAEAIRRFMGWCPNAAMAGAVRHRCAIPDAGVETVKESNR
                     EVVEGALVDYGLIGTPGRLFLLVLAGAGFIWILFVTAPASLVTVPTFVIPPAGGLLLL
                     AITLGYAALEFYGTVRRARIDIARDAITISRPPFRPIVIPKDAVETVEVKENKLPIPS
                     WFLAIALATLLVSAAGSVYAGFGNPASMRFTFGVAAAVFFPVLFYRTYLRARYPQALV
                     ITTVEKRIAVIYADDPGRIARMPGAAG"
     gene            complement(1702..2310)
                     /locus_tag="PLI76_01835"
     CDS             complement(1702..2310)
                     /locus_tag="PLI76_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1673 family protein"
                     /protein_id="HOI60801.1"
                     /translation="MSLVETIRAYLGWCPMAGPVRMDLPVRPGTVAAPGGREGALRVE
                     PGWWNRYHNQLLVMALIASAATAMLFLLIGDTSGYPVVWTGLGFGVGWTIGFLLSYRK
                     QYARITAGEFRRENMSRRQRLVRDLSVPVAAVILIAALAYIVQNGMPGWIVGFMLGLS
                     LIGWTTYGITIIWERRHRITMIAEKGSMYTLDTAIQEEMLWR"
     gene            complement(2438..3205)
                     /locus_tag="PLI76_01840"
     CDS             complement(2438..3205)
                     /locus_tag="PLI76_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60802.1"
                     /translation="MMPILETIRAYLGWCPVKGQVHRQVQPESGMRGGTPDDGRRRVG
                     GVSMKIDFIVKEELWRSIGAVYWVSFLTIGYLILSGYLPISISYAFSLALIPTVAHFL
                     IMVWYRRKENLVDPNPASILRHALGFERQSEKRTEQRRAIVEKAVDAALLVTIFLIYI
                     YASLAYSISQVYWLPVLIVISLFLARIVFTDGGVQRVTVARSVVFYLIAAGFLLLRHL
                     ALGHPVLLYLRGIVLVGIVVFPILYVWERRRTPEGTD"
     gene            complement(3202..3648)
                     /locus_tag="PLI76_01845"
     CDS             complement(3202..3648)
                     /locus_tag="PLI76_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60803.1"
                     /translation="MPLPLNDRQFAFWRLRRSGLQNIHIAERFGISRQAVSKALITMD
                     RKVEETLIAMAQANQIDVERLNAERGVLFGRSVPFDAGAIVFVSADHGVQVWYEHEGD
                     CGACPRYAQCIELLWGYADEMGIALEKTDDPTRLADELFAKLREMV"
     gene            3886..4656
                     /locus_tag="PLI76_01850"
     CDS             3886..4656
                     /locus_tag="PLI76_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HOI60804.1"
                     /translation="MDTALRDAYIRLHERYFPGTELPIAFEVGGPAEGVTRASTPKNW
                     KCFVCDLSKVRRGASLAFDEDSIGCRGGKFYLGYEGERFPDFRYFLSYGKPGVVEGER
                     YKQTPELVDELDRGAVRIPTKGKRIVSKRWDNLAAADNPDAVVFFARPEVLSGLFTLA
                     NFDRADPNGVVSPFGAGCSSIFYYPWLEQQKEDPRAVFGMFDPSARPCVPADVLTMAF
                     PMKKFERVIGFMEESFLTTDTWEKVMKKIARSNALHSP"
     gene            complement(4837..6327)
                     /locus_tag="PLI76_01855"
     CDS             complement(4837..6327)
                     /locus_tag="PLI76_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOI60805.1"
                     /translation="MVLTLEILFLGISLLFLISIIANKFGERLGVPALLIFLIVGMLA
                     GSEGPGGIPFDDPTIAQTIGVIALAYILFSGGLDTRWDEIQPVFWPAIALSTIGVVLT
                     AAVLGAFVVLILGLPPLTGFLLGAVVSSTDAAAVFSVLRMARARLKGNLRPFLEFESG
                     SNDPMAIFLTTGIIALILTPGEPVFALIPMFVQQMIVGGLFGYAMGRFVVWLINRLKL
                     ESEGLYPVLSLAMVLLTYGLTATVGGNGFIAVYIAGLVIGNSIVVYKKSLIRFHDGIA
                     WLMQIIMFLALGLLVFPSELVPVIVPGLLTATFLILVARPVAVMLTLLPWKMPLNEKA
                     LVSWVGLRGAVPVILATYPLVAGVPGADMIFNIVFFIVLVSALVHGTSIPSVARWLGL
                     AAPLQEKRKLSREFDVDPETPSELVEIAISPDAPAVGKQVVDLGLPRGALIILMQKGD
                     ERFVPGGSTVIEAGDTLLLLTTDDLVETVRARLLSGEKPGDTSRAS"
     gene            6563..7651
                     /locus_tag="PLI76_01860"
     CDS             6563..7651
                     /locus_tag="PLI76_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOI60806.1"
                     /translation="MVDTPAFSRSLGTVLLLTAAIFLIIGIQLISYLVTLIVVALILA
                     MLAYPATKALKSRGLPEGVAVGVVTVVASLLVLLLCYVVFFSFESLIQDLPLFQQELS
                     ARLAEIITTLHGFGIDTGSFSASSINLQQFAGFFSSYAMSLADLLLYLFFIAVTTIFM
                     IFEAPKMPGRIQRVNGDRSGGAETFSRMSRFLIDFVIVRTEANLVHGFLFGSFLWVMG
                     VHAAVLWGVLTFLLSYIPYIGLLVAAIPAIFFAWLQFGLWGAIAVIAAVAVLNALVEN
                     PIFAHFASRRFDIPALVVILSVIFWGWALGIAGMVFAVPLTLIVLLVAQLSADWAWVN
                     TLLGVDHLFTGGQAPPAEGRGGDVPPAE"
     gene            complement(7757..7906)
                     /locus_tag="PLI76_01865"
     CDS             complement(7757..7906)
                     /locus_tag="PLI76_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HOI60807.1"
                     /translation="MAVDPVCKMDVDPATARFTSEYKGKTYYFCAPGCKKLFDKNPES
                     YLKGS"
     gene            complement(7896..10361)
                     /locus_tag="PLI76_01870"
     CDS             complement(7896..10361)
                     /locus_tag="PLI76_01870"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOI60808.1"
                     /translation="MPEEKRKAELKISGMHCASCALNLERALKGRDDVYGARVNFATG
                     TATVEYDPAKATLADLEQTVAGAGYEVMHNEATVRIGGMVCASCAQVIEASLTDLEGV
                     YEARINLATETAHVIYNPELVTTSEIRAAVEDAGYQYLGLAEEVSEDAEALAREEDLR
                     DKFRRFTVGFVVSIPLFFYMIFGMPGAAALPVSVNVVMLIITAPVFYYVSAPIFRAAA
                     AALRNRALTMDVMYAMGIGVAYGASILGTFGLVLTPAFNFYDTAVMLAAFLTLGRYLE
                     ARAKGRTSDAIKNLIGLRPRTATVIRDDREIEVAIEEVVVDDILLVRPGEKVPVDGVV
                     VGGESAVDESMITGEPIPVGKREGDGVVGGTLNVNGVLRIRAEKIGKDMVLSQIIRLV
                     RDAQGSKPPVERIADVAVSYFIPTVLLIATAAFLAWYLALGATLLFSLTVLISVLVVA
                     CPCALGLATPTAVTVGIGRGAEFGVLIRNGEALEISEKLTAVVFDKTGTLTRGKPDVT
                     DIVAFGMPEDRLLAIAAAVEKNSEHPLGAAVVRRAEAAGVTAPASERFRAFGGRGVSG
                     AVEGEEVLVGNRSFLDEHRIPIPPEAEESMAAFQDTGKTAVFVAVSGSFAGIVAVADT
                     LKPTAKTAVADLKRKGLSVTMITGDNPRTANAIAREVGIENVHAGVLPGDKAREVRSL
                     QERGEVVAFVGDGINDAPALAQADVGIAIGSGTDVALESADIVLIRDDLTDAVAAIEL
                     SRKVMGRIKQNLFWAFAYNSALIPLAAGVLYPFFGITFRPELAALAMALSSVTVVSLS
                     LLLKTYIPPAKRGILSEVDAHGS"
     gene            10577..11473
                     /locus_tag="PLI76_01875"
     CDS             10577..11473
                     /locus_tag="PLI76_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOI60809.1"
                     /translation="MASGQDIDGLTEALQNGDPDTRRRAALALSALGEWRAVGPLIRA
                     LADPSQAVREAAANALVMVGTPAVEPLIDLLERPDFTMVYGASREETGEGLTQVDLLG
                     GPEGIPPEKRTLRHGGGITQHDAFGGPEDLKKAGGKRALEHEEEGLTQHDAFGGPEDL
                     KKAGGKQTLEHEEGGIAQHALYAGPEAIPPEKRTLPHRELAQHDLFAGPEGVREHEAL
                     FPGARKAGIVTTGVPPGMKPGLRRAYAAIILGEIADPRAEGILTRSLTDSDPAVRRAA
                     EDGLARFRERRGMATPVPPAPR"
     gene            11575..12468
                     /locus_tag="PLI76_01880"
     CDS             11575..12468
                     /locus_tag="PLI76_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOI60810.1"
                     /translation="MPIEERRPTIEVLRAEKDIAGLIEALGSDDGLTRQRAALALGDL
                     AGAEAVAPLIRALGDPLTAVREAAADSLALLGPPAVGPLVELIESPEASGKYEERTVT
                     VIGAGGRTWELETRQDLRRVYAAAILGEIGDPAAVEPLVRALRDAGDDLRYQAAGAVA
                     KFGSGAVEPLTAVLADPDPDTRIVAAGVLGDTGEAGAVEPLVGALRDGSADVRGAAGG
                     ALIRLGGAAVEPLIAATKDADRNVRLYAAGALKYIGDPRAIEPLRDLARSGDAEEKSV
                     AEDAIERIRTVRRGMPAPTSR"
     gene            complement(12646..13614)
                     /locus_tag="PLI76_01885"
     CDS             complement(12646..13614)
                     /locus_tag="PLI76_01885"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HOI60811.1"
                     /translation="MTIITRKGSTPRLPGTRMLMTRDGRRFGTVGGGFCEADAVRAAE
                     GCLASKKSCILRYSSVPGSILPSDIICGGSLEILVEYLPADPYHTRLIGDFVRLRSLR
                     QTPVMIVPLPDGSESGRVRFFIDADGLARGIYPAIHHTQVLKAAREMKRPDVFQAGGM
                     RFLIEPSPLKSRLVICGAGYIAAEIAPLAYHLGFEVSVLDDREDLLSADVYRGTERIP
                     VTSYESCLQALPVDSATDVLIVTRSHAFDLQVLRQALRSPAGYIGMIGSRHKWKTLSS
                     LLLEEGFGDDDLKRVHCPVGLEIGADSPEEIAVSIAAELIEHRALQ"
     gene            13826..14221
                     /locus_tag="PLI76_01890"
     CDS             13826..14221
                     /locus_tag="PLI76_01890"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOI60812.1"
                     /translation="MALREFLGIGCDVEETGRFHLDRTKDAKFLCRFFTAAELDYCFS
                     FEDPAPHLAARYCAKEAVEKALGAAGEEPVEYEDIEIENHSSGAPFITILAKRENLNK
                     KYTIQVSMSHCRTTAMAIATVSILPGEDP"
     gene            14256..15449
                     /locus_tag="PLI76_01895"
     CDS             14256..15449
                     /locus_tag="PLI76_01895"
                     /inference="COORDINATES: protein motif:HMM:NF018485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI60813.1"
                     /translation="MEQSTLNLNDSRRARTLFILYLTGFTTMLGIGIITPVLPIYAQM
                     IGATGFWIGAIFSVFALSRTIFMPFVGRLSDAGGRRKFILLGLAGYAFFSALYVPADN
                     VLILTIVRFFHGMAAAMVYPIANAYVADMARVGEEGKTMGGFQSAAFLGMSFGPLLSG
                     IMVDLLYVQSAFIALAGLSLTALIICIAYLPEYHVPMREIPPLRSVFFSRGMRVPIIF
                     FFTYTAGYTAFMVFFPVLAHADQMTVSGIGALIFISSISTAVFQRLSGRYADLFDRHL
                     VLSTGAGVMAVSLLLFTMSEGFLSFLLTSVLLGAGIGISLTTVSAMAVIEGRNLGQGS
                     VEGVINTAQGIGIVISPLVLGLVMDFTGISMVFLTAAGVSGLMALAMMRFVYRETPAQ
                     ARNAE"
     gene            15585..16883
                     /locus_tag="PLI76_01900"
     CDS             15585..16883
                     /locus_tag="PLI76_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60814.1"
                     /translation="MNIPETAELIVPIAIRNVPIVTGMVEQSATIFGLDKEGAQNLGL
                     AAEEVFNVLASAARPDEKIRIVVRQGGYYVEVACYFSRHAIPITALNITKIPDPDDES
                     DIENLGIILAAKVVDRLQLVRSRQGEMALRFTIERRYPEQAIPSGIPPAGHYSVGEGG
                     FEEIKQFASRVSGMYGANSPGFFRFPGKIVDMVKSREFGVLLALDGKGNVGGGMLWKW
                     GGKMIDSFGPYIFTPQPGLVGEIVQGVLEKVGRKGAVCIVNRMPGDDLLRNFFEELVE
                     SCPANRIRTTACHPPLYRQLEEDTGSVAYVHPEVEIFVKECYERLVLPRSVVATTHAG
                     EFIEPHAAFGVQVDKKQNLAVISTIVAGIDAEEVLAGYVASLYDEGITRLEFEIDLGD
                     ETTPVLVPALHAVGFIPCYVLPWGGRGDILVFEYQGPRGF"
     gene            16849..18000
                     /locus_tag="PLI76_01905"
     CDS             16849..18000
                     /locus_tag="PLI76_01905"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOI60815.1"
                     /translation="MSSNTRDREASDMAGSLPPLNRLVPPYIQKIEAYIPSKPDDVLK
                     KMYNCPFLYRMNNNENVIGPPEKAIEAIMDFDPARAAIYPSGDGYHLRHRLSEVFGLP
                     SCSFIIGNGANELIAFVIKAFCWKGDNIITSNKTFAVYEWTAEFSGFESRLVPLKDNT
                     FDDEGILKRIDDRTRVIFICNPNNPTGTYWNEARLRRFLERVDGEQIVVVDEAYAEFV
                     EQPDFPESMRLIREFPNLVVFRTFSKIYGLAALRIGYLAGDRSVVDVIRRTCVVYSVN
                     TLAQEAALAALGDDGTHIARTRSLIRASKEYLLPELDRMDLPYISGEGNFVMIRLPFS
                     DTLAYRRLMRKGYMIRTMTGFRFPNHIRVTLHDVPVMEGFVDALRNTLA"
     gene            18071..19108
                     /locus_tag="PLI76_01910"
     CDS             18071..19108
                     /locus_tag="PLI76_01910"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOI60816.1"
                     /translation="MTTEPEYTIDDFRPEDAEGVVALYRAVYGENYPIRSVYDAREII
                     RQEESHETYRMVARSPAGEIVGHVAVYHSVPPAPNQELYEAGQLMVRPDYRQTAVAFE
                     LIHACISELPQKHNIGLWGEAVCNHLFTQQMMARERFVETGLEMDLMPESSYSLPTSQ
                     AAGGRVSVIFAFRVCSPHPRTLYLPSVYAESLTFIYEEVKDEYTIALSDAGLPPGTKT
                     KGTMELFKEAGVARITISAVGSDFSDWIGRHGREAAESGIIVTQVFMPLTVPWTGAAV
                     DILRKHGYFIGGALLRWFGGDGLLMQKHAGTPNFDGAHMYTKRAKRIKELVLEDWKAV
                     TEDRRKTEDLQ"
     gene            19174..20838
                     /locus_tag="PLI76_01915"
     CDS             19174..20838
                     /locus_tag="PLI76_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOI60817.1"
                     /translation="MKENADIPADWPEKTTFGAWFRAVAAGAGDRAVLVYRDPARTVT
                     YRELDLLSDAIAKSIIAMGIEKGTHAALWAPNIQEWPAILLGCAKAGLPLLLVNTSFR
                     SYDLEYCLNLVDVGILFLASGNGQPGEYISALYEACPGLEDSQPGKISSQKLPHLRTA
                     VQIDDGNHRGILPWTAFLGQGEQVARTDLQARESSVRPEDRFIFLFTSGTTGWPKCIT
                     WTHERLIGTTFTLADGFGLAPGDVVCIPPPFFHLLGCGILLSALSSSCTVAPLERFNP
                     QEMLRTIETFRVTHIFGVPSMFVSALNEYNRTRYNTASLRGGVVAGAVSPPAVVRAVM
                     DILGAREFGICYGLSEVLATITRPSDPMECRTGSIGRPLRDTDIRIVHPLDGTDVRPG
                     ETGEILVRSPWMISGYYDKPDTIVAATDDDGFLHTGDLGFVDGDGYYHIAGRSRDLII
                     RGGENISPSEIEEFLLTHPAVMDAQVVGIKSEYYGEEPVAFVRTKPGQAVTALGLKQF
                     CRRSIAIEKVPSTFFFVDRYPLTASGKVQKFRLREMAAELLSEQKR"
     gene            20869..21120
                     /locus_tag="PLI76_01920"
     CDS             20869..21120
                     /locus_tag="PLI76_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOI60818.1"
                     /translation="MDESQIEKTLREILARRVPNAPIDKLTLDAPLAALGIDSLAYSW
                     ILADTEDTFGFVMMGSDVMKLKTLKHTIEYVQTQIEKKR"
     gene            complement(21177..22013)
                     /locus_tag="PLI76_01925"
     CDS             complement(21177..22013)
                     /locus_tag="PLI76_01925"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HOI60819.1"
                     /translation="MSEVALPASLEEVYAIRKREPGAAVLAGGTDLLVRMRADGKKPP
                     VLIGLERIPGLSGIAIQDGAVRIGPMVTHQNLLDSPVIRKHLAVMHEAAQVLGSPPVR
                     HMGTIGGNICTASPAGDLLPALYVLDAEVEISSPAKKRVVPIATFITGPGRTILTGQE
                     ILTGIRIPVPDPGYQGFYRKVGQRRALAISIVSMAALLMVSPDKTVRSARFAWGSVGP
                     VVMRFPDVESSFAGRTFGPALLADVGRQYSERIRPISDIRASASYRKMVAAGLPLKIL
                     EQ"
     gene            complement(22006..22476)
                     /locus_tag="PLI76_01930"
     CDS             complement(22006..22476)
                     /locus_tag="PLI76_01930"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOI60820.1"
                     /translation="MKIRCIVNGKQTDLETAPDRRVVDLLREDLGLCGTKEACGSGEC
                     GACTILVDGESRLSCLMIAAQLEGKEIVTIEGFSKEHLHPIQEAFIVHGAVQCGFCTP
                     GMVLAAADLLKRNPAPDRHEIRRAISGNLCRCTGYQKIVDAIESLARVEEESDE"
     gene            complement(22473..24707)
                     /locus_tag="PLI76_01935"
     CDS             complement(22473..24707)
                     /locus_tag="PLI76_01935"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HOI60821.1"
                     /translation="MTGRERYAADEYPAGLVYAGVVRSPHPHARILAIDTTGALACPG
                     VIAVLTGGDIRGTNRTGLPEMDQPVLADTKVRFTGDPVVLVVAEDRELLRQAMAAVKV
                     TYEPLPAVFDPDPALAKGAPVLHENRKEGNLLFSGVIAKGDADFAFSECAAVREGIFS
                     LPRQEHAYLETETGVAKVDGDGVLEMIVSTQSPFRDSREIAAALGIPPEKVRVIAPYL
                     GGAFGGKDGITVQGHLGLAALHSGGRPVKIWNSREESFVSSTKRHPGRVSLKLGCTKD
                     GTLHALSCRLIFDTGAYAGLGGPVAVYGMGHAAGVYRVAHSRVEGMNVYTNNPPSGAF
                     RGFGIPQVIAALEQVVDMLAESIGMDPLEFRLKNAIRVGDETGLGIRMSNSADIVTCL
                     EKARDHPLWTGRDEWIRQAPDYKRRGVGIATGVLGIGYGPVIPDIASAKLELLTDGRF
                     RIYAGVADMGQGNNSANIHIAGDILCQSPDRFELVQPDTFRCLNSSSSAASRTTYSYG
                     NALILAAGVLKERILDKVAQLHPLFRRENLILVPGAVRHTPDGSDIALHTIAEAMQEA
                     DRVATATWTAPVNRQQLAVAPDVKMYGFPHLAYTFTTHLTRVEIDEITGAVAVDAYLA
                     WTDAGRIMNPHVYEQQIQGGVIQGMGYALWEDFRVTEGAVVTKNLSTYILPTSMDVPD
                     ILSVPVETYEESGPFGMKGVGEIGIVAALPCIANAVAGAIGFRIYDAPLTPERILTAL
                     RRAA"
     gene            complement(24823..25611)
                     /locus_tag="PLI76_01940"
     CDS             complement(24823..25611)
                     /locus_tag="PLI76_01940"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOI60822.1"
                     /translation="MSIFFWKKIIEYTRRLIGQGDRAQTNYRKVEALSMDELPGADRR
                     MQGGEMKQPLCAIILAAGSSSRMGSPKAVLPLGGKTVLEHIVQTYLLAGITDIRVVTG
                     HDREQVRSVLEKIPAGEVNNPRHHEGMYSSVRCGVASCGPEISGYFIHPVDVPLITPR
                     TIRSLCVSADPAGDRIACPVYEGRRGHPPLIGASFGRAILEEDRPQGLKGFLYEYSDA
                     IVPVIVDDPGILLNMNTPADYQEVLRRMENTASDRRGRGNEKTT"
BASE COUNT         5204 a         7861 c         7764 g         4899 t
ORIGIN      
        1 ccacaccctg gcgaggtata cccccgcaac caggagaatc gggaggagcg caaacatcca
       61 gcggaggccg ggggggtacg gccccttcac ctcgaccgat ctgaaggcgt cttttccgaa
      121 ggtgagcggt cgggaacgtg acctctggac gacgacgaca tcctcaaatg actttatttc
      181 agcccgtttc gtatcgagat ggaggtgcac cgcggcatac gctataaagg agaggcctgt
      241 gaggatgagg aacacggagc gcagggatgg gatcaggaga cctgccacga agagtccgcc
      301 gaagacgagg atcgagaccg gtaagaaacg gggactggtg taatccacga atacgccctc
      361 caccgcctcc cggctgccgc cggccacggc tccgatcccg atctcgccgt cgggggcggg
      421 ataccggcgc cggacggtgc cgaccgcatt cgggcaccag cccgtccact tccgtatcgc
      481 ttcagcgaac ttcatcgtca tgcggacgcc tccccgcgga agtttgcgcg gtagatctct
      541 ttgcggtgga agaggtagat cgccatgatg atgatcccgc cgacgagcgt cgccgccccc
      601 caggtcagcg gccgccaggt gacgatatcg ccaagcaggg tctccttctc gaacttgtcc
      661 ccggcacgga tattctgcgc gtcgtagtat acggcgacgg cggagaggat gatgaggatc
      721 gggaggaact cacggtcgta cacgaccgga agaagccagg ccagagcaat gaggatcagc
      781 agcatgttct cctggtagcc gccccgatcg ccgcctgcaa tgccggccgg ctccgggccg
      841 gggttgggat ccggactctt tcccttccac gcatccccgt agcccgcttc cgcgctcgtg
      901 cgccgaacgg gtctgacggc gccggcgttc gggcaccacc cgagtttctt cctgatatac
      961 tccagaaata ccgtcatccg gccgcccccg gcatgcgggc tatccgcccg gggtcgtcgg
     1021 catagatcac cgcgatcctc ttttccacgg tcgttatcac cagggcctgc gggtagcggg
     1081 cccgcaggta ggtgcggtaa aagagcaccg ggaagaagac cgcggcggca acaccgaagg
     1141 tgaaccgcat ggaggccggg tttccaaacc cggcgtatac gctgccggcc gccgatacga
     1201 ggagcgttgc cagcgctatc gcgaggaacc aggaagggat ggggagtttg ttctccttca
     1261 cctccaccgt ctcgacggca tccttcggga tgacgatcgg ccggaaaggc ggccgactga
     1321 tggtgatggc gtcgcgggcg atatcgatac gggctcttcg tacggttcca tagaattcca
     1381 gggcggcgta acccagtgtg atagccagca gaaggaggcc ccctgccgga gggataacga
     1441 atgtcggaac cgtaacgagg gaggccggag ccgtaacaaa gaggatccag atgaagccag
     1501 cgccggcaag caccagcagg aagagcctcc ccggcgtgcc gatcaatccg tagtccacca
     1561 gtgcaccttc cacgacctcc cggttgctct ctttcaccgt ctcgaccccg gcatccggta
     1621 tggcacaccg gtgtcggacg gcaccggcca ttgccgcgtt cgggcaccag cccataaacc
     1681 tccgtatcgc ttcagcaaac ctcagcgcca tagcatctcc tcctgtatcg ccgtatccag
     1741 ggtgtacatc gatcctttct ccgctatcat ggttatccgg tgccgccgct cccagatgat
     1801 cgtaatacca tacgtggtcc acccgatgag gctcaacccc agcatgaacc cgacgatcca
     1861 gccgggcatg ccgttttgaa caatgtacgc cagagcggcg atgagaatga cggcggccac
     1921 cggtacactc agatcccgga cgaggcgttg ccgccggctc atgttctccc tgcggaactc
     1981 accggcggta atccgtgcat actgcttccg gtaactgagc aggaagccga tggtccaccc
     2041 cacgccaaag cccaggcccg tccacacgac cggatacccc gacgtatctc cgatcaggag
     2101 gaaaagcatc gccgtcgccg ccgatgcgat cagcgccatg acaagtagct ggttgtggta
     2161 gcggttccac cacccgggtt cgacccggag tgccccctcc cgcccgcccg gtgccgcgac
     2221 cgttcccggc cgcaccggga gatccatccg taccggcccc gccatgggac accacccgag
     2281 gtaggcccgt atcgtctcca cgagagacat cacgcggcct cccggagttt ttgcatgcct
     2341 gctttcggct ccgtcaaaga gcactcgttg cagaccattc tcccgaatat atcccggagg
     2401 agtacgcaga gccgatccat cgccccatgg gtcgccatca atctgttccc tccggcgtac
     2461 gccgtcgctc ccatacgtag aggatgggaa aaacgacgat accaacaagc acgattcccc
     2521 ggaggtagag aagaacggga tgccccagtg ccaggtggcg caagaggagg aacccggcgg
     2581 ctatgaggta gaagacgacc gatcgtgcga cggtgacacg ttgaacaccg ccgtcggtga
     2641 agacgatccg cgcgagaaac aggctgatga cgataaggac ggggagccag tagacctggc
     2701 tgatggagta tgcaagggat gcgtagatat agatcaggaa gatcgtgacg aggagggcgg
     2761 cgtcgaccgc cttctcgacg atggcccttc tctgctccgt tctcttctca ctctgtctct
     2821 caaacccgag ggcatggcgg aggatgcttg cgggattggg gtcgaccaga ttttccttgc
     2881 gccgatacca gaccatgatg agaaagtgcg ccacagtcgg tatcaacgca agggagaatg
     2941 cataggagat cgatatcgga aggtacccgc tcaggatgag gtaaccgatg gtaagaaacg
     3001 atacccagta aaccgcccca atcgagcgcc agagctcttc tttaacgatg aaatcgatct
     3061 tcatacttac accgccgacg cgccgtcggc cgtcgtccgg agtgcccccg cgcatgccgg
     3121 actccggttg cacctgccgg tgcacctgcc ccttcacggg gcaccacccg aggtaggccc
     3181 gtatcgtctc caggatgggc atcataccat ctcccggagc tttgcgaaga gttcgtcggc
     3241 caaccgggtc gggtcatcgg tcttctcgag cgcgataccc atctcatccg cgtagcccca
     3301 gaggagctcg atgcattggg catagcgtgg gcatgcgccg cagtcgccct cgtgctcgta
     3361 ccagacctgc accccgtggt ccgccgatac aaagacgatc gcgcccgcat cgaagggaac
     3421 cgaacgcccg aagaggacgc cgcgttcggc gttcaggcgc tccacatcga tctggtttgc
     3481 ctgcgccatc gcgataagcg tctcctcgac cttccggtcc atggtgatga gcgccttcga
     3541 gaccgcctgg cgggatatac cgaaacgctc cgcgatatgg atgttctgga gaccgctgcg
     3601 ccgaaggcgc cagaacgcaa actgcctgtc gtttaagggg agaggcatgg gtcaacatac
     3661 gcaggttgac tatatacgtg tttcgatact ctccacgaac cggggctatg tacagcgccc
     3721 ggccgccgga taacccttcg tgtgcatcac tcttcccgaa aggaccggcg aacgaccgcc
     3781 ggccgggcac gctccgggat ctccggagga gcccacgcac aggtagacgg cccgcccgac
     3841 agacagacca gaacatttaa ccccgccgtc ctgaaacagg ctcacatgga cacagcactg
     3901 cgcgatgcct acatccggct ccatgagcgc tacttcccgg gaacggagct cccgatcgcg
     3961 ttcgaggtcg gaggccccgc cgagggggta acgagagctt ccaccccgaa aaattggaaa
     4021 tgcttcgtct gcgacctctc aaaagtccgg agaggggcaa gccttgcctt cgatgaggac
     4081 tcgatcgggt gccgcggggg gaagttctac ctcggctacg agggcgagcg attccccgac
     4141 ttccgttact tcctctcgta cgggaagccc ggcgtcgtcg aaggggagcg ctacaaacag
     4201 acgcccgagc tcgtcgacga actcgaccgg ggagccgtcc ggatacccac gaagggaaag
     4261 cggatcgtct ccaagcgctg ggataacctc gcggccgcgg ataatccgga cgccgtcgtc
     4321 ttctttgccc ggcccgaagt gctctccggc ctatttacgc ttgcaaactt cgaccgggca
     4381 gacccgaacg gggtcgtctc tcccttcggc gccgggtgca gctcgatctt ctactatccc
     4441 tggcttgaac agcagaaaga ggatccgaga gcggtgttcg gtatgttcga cccctcggcg
     4501 cggccctgcg tcccggccga cgtcctgacg atggccttcc ccatgaagaa gttcgagcgg
     4561 gttatcggct tcatggagga gagcttcctc accacggaca cctgggagaa ggtgatgaag
     4621 aagatcgccc ggagcaacgc acttcactcg ccgtagggca ccggactatc cgaccctgcg
     4681 ccggagaacc gggcgggagg gatggggcgg catccgcccc catacctcgt gcggcaaagc
     4741 ccgcccggca ccgggcacac gtgacggcgg cgaagaggag cggggccaaa aagaccgttg
     4801 agcaccggca cggccgggag agcctcctgc cgctcatcac gatgcccggg acgtgtcacc
     4861 cggtttttct ccgctcaaaa gtctggcgcg gacggtctcc accagatcgt cggtcgtcag
     4921 gaggaggagc gtatcgccgg cctcgatgac ggtgctgccg cccggaacaa aacgttcgtc
     4981 ccctttctgc atgagtatga tgagcgcccc tctcggcagg ccgaggtcga cgacctgctt
     5041 gccgaccgcc ggggcatcgg gagatatggc aatctcgacc agctcgctcg gcgtctcggg
     5101 gtccacatca aactcccggg ataacttgcg cttctcctgg aggggcgcgg cgagaccgag
     5161 ccaccgggcg acggagggga tcgacgttcc atgaacgagc gccgagacga ggacgatgaa
     5221 gaagacgatg ttgaagatca tatccgcgcc cggcactccc gcgacgagag ggtaggtcgc
     5281 gaggatgacc gggacggcgc cccgcagccc cacccaggag accagagcct tctcgttcaa
     5341 cggcatcttc caggggagca gcgtaagcat gacggcgacc ggccgggcaa ccaggatcag
     5401 gaaggtggcg gtgaggaggc cgggaacgat caccggcacg agctcggagg ggaagaccag
     5461 caggccgagg gcgaggaaca tgatgatctg catcaaccat gcgataccgt catggaaccg
     5521 gatcaggctc tttttgtaga cgacgatgct gttcccaata accaggccgg cgatatagac
     5581 cgcgatgaac ccgttccctc caaccgtggc ggtcagcccg tacgtcagca gcaccatggc
     5641 aaggctgagc accgggtaga gcccttcgga ctccagtttg agacggttga tgagccagac
     5701 gacgaatctg cccatagcgt agccgaacag gcccccgacg atcatctgct gcacgaacat
     5761 cgggatgagg gcaaagaccg gctcgccggg ggtgagtatc agggcgatga tgccggtggt
     5821 gaggaagatc gccatgggat cgttgctccc cgactcaaac tcaaggaacg gacgcaggtt
     5881 tcctttcagg cgcgctcgcg ccatgcgcag gaccgagaag accgccgccg catcggtgga
     5941 cgagacgacc gcaccgagaa gaaaacccgt gagcggcggc aacccgagga tcaggacgac
     6001 aaatgcgccg aggactgctg cagtcagaac aacgccgatc gtagagagtg ctatcgccgg
     6061 ccagaagacc ggctggatct cgtcccatcg ggtatcgagg cccccggaga agaggatata
     6121 cgcaagcgcg atgaccccga tcgtctgtgc aatcgtaggg tcatcgaacg gaatgccgcc
     6181 cggtccttcg gagcccgcca gcatcccgac gatgaggaag atgaggagcg caggaactcc
     6241 gagtctctct ccgaacttgt ttgcaatgat gctgatcagg aagagcaacg atataccgag
     6301 gaacaggatc tcaagggtaa gcaccatctg tgggtacaat tcgtgcccga actatcttaa
     6361 ggatgactcc ccgctgatag accgggtacc gcgcaccgtg ccgatctttc tgcctgcagc
     6421 gcgaaacagc gccgcacaga ttcaatgtac ccgggtaacc ggtaatttac cggtcattcc
     6481 gctatattcc tgatgggccg caccgataca tttcgcggga ttgataaggt cgccgcagac
     6541 acaggtacct ggtgaaccct ggatggtaga cactcccgca ttctcccgat cgctcggtac
     6601 cgtgctcctc ttgacggcag cgatattcct gatcatcggg atccagttga tatcgtacct
     6661 cgttaccctc atcgttgttg cgctgatcct tgccatgctc gcataccccg cgacgaaagc
     6721 gctcaaatcg cgcggccttc cggagggcgt cgccgtcggc gtggtcacgg tcgtcgccag
     6781 ccttctggtc cttctgctct gttacgtcgt cttcttctcg tttgagagcc tgatccagga
     6841 tctccctctc ttccagcagg agttgagcgc ccgacttgcg gagatcatca ccaccctcca
     6901 cgggttcggg atcgatacgg gctcgttctc cgcatcctcg atcaacctgc agcagtttgc
     6961 cgggttcttc tcttcgtatg ccatgagcct tgccgacctg ctcctctacc tcttcttcat
     7021 agcggtgacg acgatcttca tgatctttga agcgccaaag atgcccggcc gcatacagag
     7081 ggtgaacgga gaccggtcag gaggcgccga gacgttctcc cggatgagca ggttcctgat
     7141 cgacttcgtc atcgtcagga ccgaggcgaa cctcgtccac ggtttcctct tcggttcgtt
     7201 cctctgggtc atgggggtgc atgctgccgt cctctgggga gtgctgacgt tcctgctctc
     7261 ttacatcccc tacatcggcc tgctcgtcgc agcaatcccc gctatcttct ttgcctggct
     7321 gcagttcggg ttatggggcg cgattgcggt gatcgctgcc gtcgccgtct tgaacgcgct
     7381 tgtggagaac ccgatatttg cacactttgc atcgaggcgc tttgacatcc ccgccctcgt
     7441 ggtcatcctc tcggtcatct tctgggggtg ggcgcttggc atcgccggga tggtgttcgc
     7501 cgttccgctc acgctcatcg ttctcctggt cgcccagttg agcgccgact gggcgtgggt
     7561 gaacacgctc ctgggcgtcg atcacctctt tacgggaggg caggcacccc ctgcggaggg
     7621 cagggggggc gacgtgccgc cggcagagtg atcatcccct ataccatcct cgcgtgggca
     7681 aaacgccggc cggtgccgcg cccgcgcatc ggttcgatac cgggcggatc taatccccgc
     7741 ccggcacaaa aaaaggtcag gaccccttca ggtaagattc cgggttctta tcgaagagct
     7801 tcttgcaccc cggagcgcag aagtagtagg tcttcccctt atactcgctc gtgaacctcg
     7861 ccgtcgccgg atcgacgtcc atcttgcata ccggatcaac tgccatgcgc atcaacctcc
     7921 gatagtatcc ctctcttcgc cggaggtata tatgttttga gcagcagcga gagcgagacg
     7981 accgtcaccg acgagagggc catcgcgagc gccgcaagct ccggccggaa ggtgatgccg
     8041 aagaaggggt agagcacgcc cgcggcgagg gggatgaggg ccgagttgta ggcaaacgcc
     8101 cagaagaggt tctgcttgat ccggcccatc accttccggg agagctcgat ggccgcgacg
     8161 gcgtcggtga ggtcgtcgcg gatgaggacg atatcggcgc tctcgagggc gacgtcggtc
     8221 ccgctcccga tcgctatccc gacgtccgcc tgcgcgagcg ccggtgcatc gttgatcccg
     8281 tcgcccacga acgccacgac ctcgccgcgc tcctggaggc tccggacctc cctcgcctta
     8341 tccccgggca gcacccccgc gtggacgttc tcgataccga cttcacgtgc gatggcgttc
     8401 gccgtccgcg ggttgtcgcc ggtgatcatc gtcaccgata ggccctttct cttcaggtcc
     8461 gcaacggcgg tcttcgccgt cggtttcagc gtatcggcaa cggcgacgat gccggcaaaa
     8521 ctgcccgaga ctgcaacgaa gaccgcggtt tttcccgtat cctggaatgc ggccatactc
     8581 tcctccgcct cgggcggtat gggaatgcgg tgctcatcga ggaaggaccg gttcccgacc
     8641 aggacctcct cgccttccac cgcgccgctc acccctctgc cgccgaatgc ccggaatctc
     8701 tccgacgccg gggcggtgac gccggccgcc tccgcccgcc gcacgaccgc cgccccgagc
     8761 gggtgctcgg agttcttctc gaccgccgcc gcgattgcga gcaaccggtc ctcgggcata
     8821 ccgaacgcga cgatatcggt gacgtcgggt ttcccccggg tgagcgtccc ggtcttgtcg
     8881 aagacgaccg cggtcaactt ctccgagatc tcgagcgctt cgccgttccg gatcagcacg
     8941 ccgaactcgg cgcccctccc gatcccgacc gtcacggcgg tcggcgtggc aagcccgagg
     9001 gcgcaggggc aggcgacgac caggaccgat atcaggaccg tgagcgagaa caggagcgtg
     9061 gcgccgaggg ccaggtacca ggcgaggaaa gcagcggtcg cgatcaaaag gacggtcggt
     9121 atgaagtaag agaccgcgac gtcggcgatc cgctccaccg ggggcttcga gccctgggcg
     9181 tcacggacga gccttattat ctgcgagagg accatatcct tccctatctt ctcggcccgg
     9241 atccggagga cgccgttcac gttcagggtg ccgccgacga ccccgtcgcc ctcccgtttg
     9301 ccgaccggga tgggttcgcc ggtgatcatc gattcgtcga ccgcgctctc gccgcccacg
     9361 accacgccgt ctaccggcac cttctccccc ggcctgacca ggaggatatc gtcgacgacg
     9421 acctcctcga tggcgacctc gatctcccgg tcatcgcgga tcaccgtcgc ggtccggggc
     9481 cggagcccga tgaggttctt gatggcgtcg gacgtccgcc ctttggcccg ggcctcgaga
     9541 tagcgcccga gtgtcaggaa cgcggcgagc atcaccgccg tatcgtagaa gttgaacgcc
     9601 ggggtgagga cgagcccgaa cgtcccgagg atgcttgcgc cgtaggcgac accgatgccc
     9661 atagcataca tgacgtccat cgtcagggcc ctgttccgga gagccgcggc ggccgccctg
     9721 aatatggggg cgctgacgta gtaaaagacg ggtgcggtga tgatgagcat gaccacgttc
     9781 acggagaccg ggagagcggc cgcccccggc atcccgaaga tcatgtagaa gaagaggggg
     9841 atgctcacca cgaacccgac ggtgaaccgg cggaacttgt ccctgaggtc ttcctcccgc
     9901 gccagggcct cggcgtcttc ggagacctcc tccgcaagcc cgaggtactg gtagccggca
     9961 tcctcgacgg cggcccggat ctcggatgtg gtgacgagtt cggggttata gatgacgtgt
    10021 gcggtctcgg tcgcaaggtt gatccgtgcc tcgtagaccc cctcgaggtc ggtgagcgat
    10081 gcctcgatca cctgggcgca ggatgcgcag accatcccgc ctatcctgac ggtggcctcg
    10141 ttatgcatga cctcgtaacc ggcaccagcg accgtctgtt cgaggtctgc aagggtggcc
    10201 ttcgccggat cgtactcgac cgtggccgtc ccggtcgcga agttcacccg ggccccataa
    10261 acgtcgtcac ggcccttgag ggcacgttcg agattgaggg cacaggacgc gcagtgcatc
    10321 ccggagatct tcaactctgc ctttcgtttc tcctccggca tgatgatctt ctcgtccggt
    10381 gaagataaat acctgcacct ctcatctccg ggcctgtaca gccgcacgtg ggcgacgaaa
    10441 atctccggta tgggcgcgcc ggcatttata cgcaacatta ttatataagc ccctggatat
    10501 taccgcccat cctgaggtga tcgtggtgca gggaaggaat aatccagaag gagagaagcg
    10561 acgggatgtt gagcagatgg cgtcaggaca ggatatcgac ggcctgaccg aggcgttgca
    10621 gaacggcgat ccggacaccc gccggagagc cgccctcgcc ctcagcgcgc tcggggaatg
    10681 gcgggcggtg ggccccctca tccgcgcgct cgccgacccg tcacaggctg tccgggaggc
    10741 agcggcgaac gcgctcgtga tggtcgggac gccggcggtc gagccgctga tcgacctcct
    10801 cgagcgtcct gactttacga tggtctacgg agcgtcccgg gaagagaccg gggaggggct
    10861 gacccaggtc gacctcctcg ggggacccga aggcataccg cccgagaaga ggacgctccg
    10921 gcacgggggg ggcattacgc agcatgatgc gttcggcggg ccggaggatc tcaagaaagc
    10981 cggcgggaag cgggcgctcg agcacgaaga ggaagggctt acgcagcacg atgcgttcgg
    11041 cggacccgag gatctcaaga aggccggcgg gaaacagacg ctcgagcacg aagagggggg
    11101 gatcgcccag cacgctctct acgcgggccc cgaggccata ccgcccgaga agcggaccct
    11161 cccacaccgg gagcttgcgc agcacgacct ctttgccggc cccgagggcg tgcgtgagca
    11221 cgaggcactc ttcccggggg cgaggaaggc cggcatcgtg acgaccgggg tgcccccggg
    11281 catgaaaccg gggctgcgga gggcttacgc cgccatcatc ctcggcgaga tcgccgatcc
    11341 gcgggcggag gggatcctca cccggtcgct caccgacagc gaccctgccg tccggagggc
    11401 ggccgaggac ggactcgcgc ggttccggga gcggcggggt atggcgacgc ccgtcccgcc
    11461 ggcaccccgc tgatacccct ccattttcac ggtcccccgg aaccggacaa ggttcttata
    11521 ccgggcaacc atcatacgcc ggatccggag gcgaagaaat tgccggaaaa gagcgtgccc
    11581 atagaagaga ggcgcccgac gatcgaggtt ctgcgggcgg agaaggatat cgccggcctg
    11641 atcgaggcgc tcgggagcga tgacggcctg acccgccagc gggccgccct ggcgctcggg
    11701 gatctcgccg gtgccgaagc ggtcgccccc ctcatccggg cgctcggcga tccgctgacg
    11761 gcggtgcggg aggcggcggc ggactcgctt gccctgctcg gccccccggc ggtcgggcct
    11821 ctggtcgagc tcatcgagag ccctgaagcg tccgggaagt acgaggagcg cacggtgacc
    11881 gtgatcgggg ccggcggccg gacctgggag ctcgagaccc ggcaggacct ccggcgggtg
    11941 tatgccgccg ccatacttgg cgagatcggc gatccggccg cggtagaacc gctcgtccgg
    12001 gcgctccgcg acgcaggcga cgacctccgg taccaggcgg cgggagccgt agcaaagttc
    12061 ggttctggag cggtagaacc gctcacggcg gtgctcgccg accctgaccc cgatacccgg
    12121 atcgtcgcgg ccggcgtcct cggggatacc ggggaggccg gagcggtcga gccgctcgtc
    12181 ggtgcgctcc gtgacgggag cgccgacgtc aggggggcgg cgggcggcgc cctcatccgc
    12241 ctgggcggtg cggcggtcga accgctcatc gccgcgacga aggatgccga ccgaaacgtc
    12301 cggctctacg cggcgggggc gctcaagtac ataggcgacc cgcgggcgat cgaacccctc
    12361 cgggacctcg cccggagcgg cgatgcggag gagaagagtg tcgcggaaga cgcaatagag
    12421 aggatccgga cggtgcggag ggggatgccg gcgccgacgt cgcggtaacc atccaatttt
    12481 tctgcttttt cgccggatcg tgcaccataa aggattgcct gaagcagtcc cgcgcccggc
    12541 gtttatccgg ccgcggtata agagaacgag tgtcgtcgaa gcggcggggt ctatcgtggt
    12601 ctccggtcaa acgacccata accgtcaaga acgccggaat gccgattatt gaagagcacg
    12661 gtgttcgatc agctccgctg caatgctcac cgcgatctcc tcgggggaat cggcaccgat
    12721 ctcgagaccg accgggcagt gcacccgttt caggtcgtca tcaccaaatc cttcctccag
    12781 caaaagcgaa gaaagagtct tccatttgtg cctgctgccg atcatcccta tataccctgc
    12841 cggactccgg agggcctgcc ggagcacctg gaggtcgaag gcatggctcc gggtcacaat
    12901 caggacgtcg gttgccgaat ccacgggcag agcctgaagg caggattcgt acgacgtaac
    12961 gggtattcgt tccgttccac gatacacgtc cgcactgagg agatcctcac ggtcatccag
    13021 cacggatacc tcaaatccca ggtggtatgc aagaggagcg atctctgctg cgatgtagcc
    13081 ggcaccacag ataacaagcc ggctttttaa ggggctgggc tcgatcagga accgcatccc
    13141 gccggcctga aagacatccg gccgtttcat ctcccgggcg gctttcagca cctgcgtatg
    13201 atgaatcgcg gggtatatcc cccgggccaa cccgtctgca tcgatgaaga accttacccg
    13261 accgctttcg ctgccatccg ggaggggcac gatcatcacc ggggtctgcc gcaggctccg
    13321 gagccgtacg aaatcgccga tgagccgggt gtgataggga tcggcaggga ggtactcgac
    13381 aaggatctca agcgatccgc cgcagatgat atcagaaggc agtatcgatc cggggacgga
    13441 ggaataccgc aggatgcaac tttttttaga ggcgagacac ccttctgcgg cccggacagc
    13501 atcggcttca cagaaaccgc cgccgacggt cccgaaccgt ctcccgtccc gggtcatgag
    13561 catccgggtt ccggggagcc ggggcgtgga gcctttccgg gtgatgatcg tcatgacggc
    13621 aagatccccg ctcgtttcca gcaggttgct gatggtacga taatcgctgc tctctgtcgg
    13681 gttcagggta accagcctcc ggataattcc tggttacagg ttgttgatag aatatccata
    13741 cggaaaagca taataactat ctgtcggatc agatctttat accggaacgt acggatatca
    13801 ccgggcggcc cggcgggaat gcggcatggc cttgcgggaa tttttgggaa tcggctgcga
    13861 cgtcgaggag acgggcaggt tccaccttga caggacaaaa gatgcaaaat ttctatgcag
    13921 gttcttcacc gcagcggagc tggattactg cttctcgttc gaggaccccg cgccacacct
    13981 tgccgcccga tactgtgcaa aagaggccgt ggaaaaagcg ctcggcgcag caggagaaga
    14041 gccggtcgag tacgaagaca tagagatcga aaaccactca tccggagcgc ctttcatcac
    14101 tatcctcgca aaaagagaga atcttaacaa aaagtatacg atccaggtga gcatgtcgca
    14161 ctgcaggacg actgccatgg caatagcaac tgtatcgata ctccccgggg aagatccctg
    14221 aaacctgcac atcctcataa atagtgtcgt gggatatgga gcagagtacc ctgaacctga
    14281 atgattcccg gcgagcgcgg acgctcttca tcctgtacct gaccgggttc accacgatgc
    14341 tgggtatcgg catcatcaca ccggtcctcc cgatctatgc ccagatgatc ggtgccacgg
    14401 ggttctggat cggggcgata ttctcggtat ttgccctgtc ccgtaccatc ttcatgccgt
    14461 ttgtcggcag gctgtcggat gcgggaggga gaaggaagtt tatcctgctc ggccttgccg
    14521 ggtatgcctt cttctccgca ctgtatgtcc cggcggataa tgtcctcatc ctgaccattg
    14581 tccggttttt tcacggaatg gcagcagcga tggtctatcc catcgccaat gcctatgtcg
    14641 cggatatggc gcgggtaggt gaagagggaa agacgatggg agggttccag agtgctgcat
    14701 tcctcgggat gagcttcggt ccgcttctct caggcataat ggtggatttg ttgtatgtcc
    14761 agagtgcttt tatcgcactt gcgggactgt cgctgacagc tctgatcatt tgcatagcgt
    14821 acctcccgga gtatcatgtc ccgatgagag agatcccgcc gctcaggtcg gtctttttca
    14881 gcagaggaat gcgagttccc atcatctttt tctttaccta cacggcggga tacacggcgt
    14941 ttatggtctt ctttccggtt ctcgctcacg cagaccagat gaccgtcagc gggatcggag
    15001 cactcatctt catctcctcg atctcaacag cggtcttcca gcggctttcc gggagatatg
    15061 ccgatctgtt cgacaggcac ctggtcctct ccaccggggc cggggtcatg gcggtgtctc
    15121 tgctgctttt cacgatgagc gagggctttc tttccttcct cctcacctcg gtgctcctgg
    15181 gtgccggcat cggtatatcg cttacgaccg tatcggcaat ggcggtcatt gaaggaagga
    15241 atcttggcca gggaagtgtt gaaggggtca tcaacacggc gcaggggatt ggtatcgtta
    15301 tcagcccgct cgtcctggga ctggtcatgg actttacagg aatatcgatg gtatttctca
    15361 cagccgcagg agtatcgggg ctgatggcac tcgcaatgat gcggtttgta tatcgggaaa
    15421 cgcccgctca ggcccggaat gcagaataga aagattgata ttgccgctgc ataattgata
    15481 cacctattgg aaccgcggga ccaaaacagg catgccggtt ccgtgaatca cgaagatcat
    15541 cgggtgttta ttctcacaat aaagctcagg aatggttagc catcatgaac atacctgaaa
    15601 cagcagaact catcgtcccg attgcgatcc ggaatgtccc tatcgtcacc ggcatggtcg
    15661 aacaatcggc aactattttt ggcctggaca aagagggcgc ccagaacctc gggcttgcag
    15721 cggaagaggt gttcaatgtc ctggcatctg ccgcaaggcc tgatgaaaag atccggatcg
    15781 tcgtcagaca gggcggctac tatgtggaag ttgcatgtta tttctcgcgc catgcaatcc
    15841 cgatcaccgc gctgaacatc accaaaatac ccgatcctga cgatgaatcg gacatcgaaa
    15901 acctcggcat catccttgcc gccaaggtgg tcgaccggct ccagctggtc aggagccgtc
    15961 agggagagat ggctctccgt ttcactatcg agcgacggta tccggagcag gccatccctt
    16021 ccgggatccc gcctgcgggg cattattcgg tcggggaggg agggttcgaa gagatcaagc
    16081 agtttgcttc acgtgtctcg gggatgtatg gtgccaattc ccccggattc ttccggttcc
    16141 cgggaaagat cgtggatatg gtgaagagcc gggaattcgg tgttctcctg gcccttgacg
    16201 gaaaaggcaa cgtcgggggc gggatgctgt ggaaatgggg ggggaagatg atcgactcct
    16261 ttgggcccta catctttaca ccacaacccg ggcttgtcgg tgagatcgtg cagggagtac
    16321 tggaaaaagt gggcaggaag ggtgcagtct gcatcgtgaa caggatgccg ggcgacgatc
    16381 tcctccgcaa tttctttgag gaacttgtcg aaagctgccc tgcaaacagg atccggacca
    16441 cggcatgcca cccccccctg tatcgccagc tggaggagga tacggggtcc gttgcatatg
    16501 tccatcccga agtagagatt tttgtaaaag aatgctatga acggctcgtg ctgccccgga
    16561 gcgtggtcgc cacgacccat gcaggagagt tcatcgagcc tcacgcggca ttcggcgtcc
    16621 aggttgataa aaaacagaac cttgcggtca tttcaacgat agtcgcagga atcgatgcgg
    16681 aggaagtcct tgcagggtac gtcgcctctc tgtacgacga ggggataacg cggctcgagt
    16741 tcgagatcga tctcggggac gagacgactc cggtgctggt tcctgctctc catgccgtgg
    16801 gattcattcc ctgctatgtc ctgccctggg ggggtagagg ggatattctt gtcttcgaat
    16861 accagggacc gcgaggcttc tgatatggcc ggcagccttc ccccgctcaa ccgactcgtc
    16921 ccgccatata tccagaagat cgaggcatat atcccgagca agccggacga tgtcttaaaa
    16981 aaaatgtaca actgcccgtt cctgtaccgg atgaacaaca acgagaacgt gatcggccct
    17041 ccagagaaag ccatcgaggc gatcatggat ttcgatccgg cgagagcggc aatctaccca
    17101 agcggcgacg gctaccacct gcgccaccgg ctcagtgaag ttttcggcct cccgagctgt
    17161 tcgttcatca tcggtaacgg tgcaaacgaa ctgattgcat ttgtcattaa ggcattctgc
    17221 tggaagggag ataatatcat cacgtccaat aagacgtttg ccgtttacga gtggacagcg
    17281 gagttttccg ggttcgaaag ccggcttgta ccgctcaaag acaacacctt cgatgacgaa
    17341 ggaatcctga aacggattga tgacaggacc agggtgatct tcatctgcaa ccccaacaac
    17401 cccacgggca cgtactggaa tgaggccagg ttgagaaggt tcctggaacg cgtcgacggg
    17461 gagcagatcg ttgttgttga cgaggcttat gccgagtttg tcgaacaacc ggattttccg
    17521 gagagtatgc gtctcatccg ggagttcccc aacctcgtcg tcttccggac gttctcaaag
    17581 atatacggac ttgcagccct gcgcatcggt tatcttgccg gggacagaag cgtggtcgac
    17641 gtcatccgcc gcacctgcgt ggtctattcc gtgaacacgc ttgcacagga ggccgcgctc
    17701 gcggcgctgg gggacgacgg cacccatatc gcacggacac ggagcctgat ccgggcatcg
    17761 aaggaatatc tcctgcccga actggaccgg atggacctcc cgtacatcag cggggaaggc
    17821 aatttcgtca tgatccgcct gccgttctcc gatacgctcg cttaccggag gctcatgcgg
    17881 aaaggataca tgatccggac catgaccggg ttccgattcc ccaaccatat ccgggtcacc
    17941 ctgcacgacg tgccggtgat ggaagggttc gtggatgcac tcaggaacac gcttgcctga
    18001 cacaacgtgc ggatgcagta gacccatttc ggtatagcat gagatttaat gatgaacaag
    18061 ggatgaaaaa atgacgaccg aaccagaata cacgatcgat gatttcaggc cggaggatgc
    18121 agagggcgtc gttgcactct accgcgccgt gtacggcgaa aattacccga ttcgcagtgt
    18181 atacgatgca agggagatca tccggcagga agagagccac gagacctacc gcatggtagc
    18241 ccgttcaccg gccggtgaga tcgtcgggca tgtcgctgtt taccattcgg tgcccccggc
    18301 cccgaaccag gagctgtacg aggccgggca acttatggta cgtccggatt accggcagac
    18361 ggctgttgcg ttcgagttga tacatgcctg tataagtgag ctcccgcaaa aacacaacat
    18421 cgggctctgg ggagaagcgg tctgcaacca cctctttact cagcagatga tggcgcggga
    18481 aagattcgtg gagaccgggc ttgagatgga tctgatgccg gaatcctcct acagcctgcc
    18541 gacatcccag gctgccggcg gccgggtatc ggtaatcttc gccttccgtg tatgttcacc
    18601 gcatccccgg acactgtacc tgccatcggt ttacgctgaa tctcttacat tcatctacga
    18661 ggaagtgaag gatgaataca ctatagcact ctccgatgct ggcctgcctc caggtacgaa
    18721 aacgaagggc acgatggagc tattcaaaga agccggggta gcccggatca ccataagtgc
    18781 cgtcggcagc gatttctccg attggatcgg gaggcacggg agagaggctg ccgaaagcgg
    18841 cattatcgtc acccaggtct tcatgccgct cacggtgccg tggaccggtg cggcggtcga
    18901 tatcctgcga aagcatggat acttcatcgg gggagcgctc ctccggtggt tcggcggcga
    18961 cgggctcctc atgcagaaac atgccggcac cccgaatttc gacggggcgc atatgtacac
    19021 gaagcgggca aaaaggatca aggagctcgt gctcgaagac tggaaagccg tgaccgaaga
    19081 tcggaggaag acggaggatc tgcaatgaaa gagaatgccg acgttccggc cgaccgtcag
    19141 gggagagaca aacgtcccta tcgggaacct gcaatgaaag agaatgccga tattccggcc
    19201 gactggccgg agaagacgac gttcggtgca tggttccgtg ccgtggcggc aggggccgga
    19261 gacagggccg tgctcgtgta ccgcgatcct gcacggacgg tcacctaccg tgaactggat
    19321 ctgctctcgg atgcgatagc aaaaagcatc attgccatgg gtatcgaaaa gggcacgcat
    19381 gctgccctct gggcaccgaa catccaggaa tggccggcca tccttctcgg atgtgcaaaa
    19441 gccggccttc ctctcctcct tgtgaatacc agtttcagat cctacgatct ggagtactgc
    19501 cttaatctgg tggatgtagg tattctgttc cttgcttccg gaaacgggca gccgggcgaa
    19561 tatatctcgg cgctgtacga agcctgcccc ggacttgagg attctcaacc ggggaagatc
    19621 tcctcgcaaa aactgccgca cctcaggacc gcggttcaga ttgacgatgg gaaccatcgg
    19681 ggcatcctgc cctggacggc attcctcggg cagggagagc aggttgcccg aacagatctt
    19741 caggcacggg agagttccgt aaggcccgaa gatcgcttca tcttcctttt tacctcgggg
    19801 acaaccggct ggccgaagtg cataacctgg acacatgaac ggttaatcgg aacaacgttc
    19861 actcttgcgg acggctttgg tctcgcaccg ggcgatgtcg tctgcattcc gcccccgttc
    19921 tttcatttac tcgggtgtgg gatattgctg agcgcacttt ccagcagctg cacggtagcg
    19981 cctctcgaac ggttcaaccc gcaggagatg ctgaggacca tcgaaacttt tcgtgtcacg
    20041 catattttcg gggtgccttc catgttcgtg agcgccttaa acgagtacaa ccgaacccgc
    20101 tacaacacgg cgtcgctcag ggggggtgtg gtcgcggggg ctgtctcccc cccggccgtc
    20161 gtcagggcgg tcatggatat attgggagcg cgggagttcg ggatctgtta tggactcagc
    20221 gaggttctag cgaccatcac ccgtccgtcc gatcctatgg agtgcaggac aggcagcatc
    20281 gggagaccgc tgagagacac cgatatacgg atcgtccatc ccctggacgg tacggacgtc
    20341 aggcccgggg agaccgggga gatccttgtc aggagcccct ggatgatatc cggctattac
    20401 gataagcccg atacgatcgt tgctgcaacc gacgacgacg ggttcctcca taccggcgac
    20461 ctcggctttg tggacgggga cggctactac catatcgccg gccggagcag ggatctgatc
    20521 atccgcggcg gggagaatat atctccctcg gagatcgagg agttcctcct cacccaccct
    20581 gccgtcatgg atgcacaggt ggtcgggatc aagagcgaat attacggtga ggagccggtg
    20641 gcattcgtcc gcacgaaacc cgggcaggcg gtaacggccc tcgggctcaa acagttctgc
    20701 cggcgcagta ttgccatcga aaaagtccct tctactttct tctttgtcga ccggtacccc
    20761 ctgacggcaa gcgggaaggt gcagaagttc cggctcaggg agatggcagc cgaattactc
    20821 tccgaacaaa aaagatgaat atatattcta cgagagagtt ttagaaatat ggatgaatct
    20881 cagattgaga agaccctgcg ggagatactt gcccgccgcg taccgaacgc cccgatcgat
    20941 aaactcaccc tggacgcacc ccttgcagcg ctgggaatcg attcgctcgc gtactcgtgg
    21001 atccttgccg atacggaaga tactttcgga ttcgtcatga tgggctcgga tgtcatgaaa
    21061 ctcaaaaccc tcaaacatac gatcgagtac gttcaaacac agatcgagaa gaagagataa
    21121 acccggacgg cagcctcgtt gaggcgatgc cggagggtgc ccgtagccgc cttttttcac
    21181 tgctcaagga tcttcagggg caggccagcc gccaccatct tccggtacga ggcagaagcc
    21241 cgtatatcgc tgattggccg gatccgttcg gaatattgcc tcccgacatc tgcaagaagc
    21301 gccgggccaa acgtcctgcc ggcaaaggat gattcaacgt ctggaaaccg catgacaacc
    21361 ggtcccacgc tcccccaggc aaaccgggca gaacgaacgg tcttatccgg ggagaccatg
    21421 agcagggctg ccatgctgac gatggatatt gcgagggctc tccgctggcc caccttccgg
    21481 taaaatccct gataccccgg atcgggaaca gggatcctga tcccggtaag gatctcctgg
    21541 ccggtcagga ttgtcctccc cgggccggtg ataaacgttg caatagggac aacccgcttt
    21601 ttggcaggag aagatatctc cacttcagcg tccagcacat acagggcagg aagaagatcg
    21661 ccggcaggcg aggcggtgca gatgtttccc ccgatcgtac ccatatggcg taccggcggc
    21721 gatccgagaa cctgtgccgc ctcgtgcata accgcgaggt gtttcctgat taccgggctg
    21781 tcgagcaagt tctggtgagt gaccatcggc ccgattcgga cggccccatc ctgtatcgct
    21841 atccctgaaa gcccgggtat ccgctcaaga ccgatcagga ccggcggctt ttttccgtca
    21901 gcccgcatcc gcacgaggag atccgtccct ccggcaagca ccgcagcacc gggttcgcgc
    21961 ttccggatcg catacacctc ttcgagcgat gcgggaaggg ccacctcact catcggattc
    22021 ctcctctacc cgggccaggg attcgatggc atcgacaatc ttctggtatc cggtgcatcg
    22081 acagaggttc ccgcttatcg ctctcctgat ctcatgccgg tccggcgcgg gatttctttt
    22141 cagaagatct gctgctgcaa ggaccatgcc cggcgtgcag aacccgcact ggactgcccc
    22201 atgcacgatg aacgcttcct ggatcgggtg caggtgctct tttgaaaaac cttctattgt
    22261 cacgatctcc tttccttcaa gctgggcggc aatcatcagg caggagagac ggctctcccc
    22321 atcgacgaga atggtgcagg cgccgcattc ccctgacccg caggcttcct ttgtcccgca
    22381 gaggcccaga tcctcccgga ggaggtcgac gacccttcgg tcgggagccg tttcaagatc
    22441 ggtttgcttt ccgttgacga tgcatcggat cttcatgctg ccctccggag agcggtaagg
    22501 atccgctccg gcgtaagcgg ggcatcgtag atcctgaacc cgatagcccc ggcaacagca
    22561 ttcgcgatgc aggggagtgc cgcgacgata ccgatctccc caaccccctt catgccaaaa
    22621 ggcccgctct cctcataagt ctccacggga acggacagga tgtcagggac atccattgag
    22681 gtgggcagga tgtaggtcga gaggttcttc gtcacaacag ccccttccgt tacccggaaa
    22741 tcctcccata gtgcataccc catgccctgg atgactcctc cctggatctg ctgctcgtat
    22801 acatgaggat tcatgatccg cccggcatcc gtccaggcga ggtatgcatc gacggccacc
    22861 gccccggtga tctcgtcgat ctctaccctg gtcaggtgcg tggtgaacgt ataagcaagg
    22921 tgggggaaac catacatttt aacgtcgggt gcaacggcaa gctgttgccg gttgaccggc
    22981 gcggtccagg ttgctgttgc aaccctgtcc gcctcctgca ttgcttctgc gattgtatgg
    23041 agtgcgatat cgctcccatc cggagtatgc cggaccgccc cgggcacaag gataaggttc
    23101 tctctccgga agaggggatg cagctgcgcg accttatcca ggatccgttc tttcagcacg
    23161 cctgctgcga ggataagggc attcccgtac gagtaggtcg tcctgcttgc ggccgaggac
    23221 gatgaattca ggcaccggaa cgtgtccggt tggaccagtt cgaaccggtc cggggactgg
    23281 cagaggatat ccccggcgat atggatgttt gccgaattgt tgccctggcc catatccgcg
    23341 acccccgcat agatcctgaa ccggccgtcg gtgagaagtt ccagttttgc gctggcaata
    23401 tcgggaatga ccggaccgta cccgatgccg agaactcctg tcgcaatgcc gacccctctc
    23461 cttttgtaat cgggggcctg ccgtatccac tcgtcccggc ctgtccagag cgggtgatcc
    23521 ctggcctttt caaggcaggt gacaatatcg gcggagttgg acatccggat tccaagaccg
    23581 gtctcatcgc ccacacgtat cgcattcttc agcctgaact ccagcggatc catcccgata
    23641 ctctccgcaa gcatatccac gacctgttca agggctgcga tcacctgggg aatcccgaac
    23701 ccccggaatg ctcccgatgg gggattattg gtgtacacgt tcatcccttc cacacggcta
    23761 tgagcgacac ggtatacccc tgccgcatgc cccatgccgt atacggcgac cgggccgcca
    23821 aggccggcat aggcgccggt atcaaagata agccggcagg aaagggcgtg cagggtgccg
    23881 tcctttgtgc atcccagctt caaggatacc cggccgggat ggcgttttgt cgagctgacg
    23941 aaactctctt cgcgcgaatt ccatatcttg accggccttc ccccggagtg gagggccgca
    24001 aggccaaggt gcccctgcac cgtgatgccg tccttgccgc caaacgcacc gccgagatac
    24061 ggggcgatta cgcggacctt ttcgggtgga ataccgagag ccgcggcaat ctcccgggaa
    24121 tcccggaacg ggctctgggt cgatacgatc atctcgagca ccccgtcccc gtcaaccttt
    24181 gccacgccgg tctcggtctc aagatacgca tgctcctgcc gcgggaggga gaagataccc
    24241 tcccggacag ctgcacattc ggaaaatgca aaatccgcat ctcccttcgc gatcacgccg
    24301 gagaagagca gatttccttc cttacggttt tcatgcagga cgggggctcc ctttgcgaga
    24361 gccgggtccg ggtcgaatac tgcagggagg ggttcatacg tcaccttgac cgcagccatt
    24421 gcctgcctga ggagctctct gtcctcagca acgaccagca ccaccggatc cccggtaaac
    24481 cggacctttg tatcggcaag gacgggttgg tccatctccg gaaggccggt ccggttcgtc
    24541 ccccggatat ccccgcctgt caggacagcg atgacaccgg ggcaggcaag cgcccctgtc
    24601 gtatcgatag cgaggattcg ggcatgcggg tgcggagacc tgacaacccc ggcatacacc
    24661 agcccggcag ggtattcatc tgctgcataa cgctccctgc cggtcacctt tccataagca
    24721 tcggcccgaa agagatctga tccgaccggg accggcaggt cacttttcgt atgaaaatgc
    24781 ccttcccctt tttcatccgg tatgcctgtg gccccatggg catcatgtag ttttttcatt
    24841 gccccttccc cggcgatcgc tggcagtatt ttccattctc ctgagcactt cctggtaatc
    24901 cgcgggtgta ttcatgttca aaaggattcc cggatcgtca acgattaccg gaacgattgc
    24961 atccgaatac tcataaagaa atcctttgag gccttgtggg cggtcttctt caaggatcgc
    25021 tcttccaaac gatgccccga tgagcggggg atggcctcgc cgcccctcat acaccgggca
    25081 tgcgatacgg tcaccggcag ggtctgcgga tacgcaaaga gacctgatgg tccggggcgt
    25141 gatgagcggg acgtcaaccg gatggatgaa atacccggag atctccgggc cgcacgaggc
    25201 aaccccgcat cggacggacg agtacatccc ctcgtggtgc cgggggttat tgacctcccc
    25261 ggccgggatc ttctcaagaa cggatcggac ctgttcgcga tcatgcccgg tcactacccg
    25321 gatatccgta attccggcga ggagataggt ttgaacgata tgttccagaa ccgtctttcc
    25381 tccaaggggg agcacggctt ttggagagcc catccgggac gatgagccgg ctgcaagaat
    25441 aattgcgcac aacggttgct tcatttcccc tccctgcatc ctcctatccg ctcctggcag
    25501 ttcatccatc gataacgctt caactttcct gtaatttgtc tgagcccggt ctccctgtcc
    25561 gatcagtctt cgcgtatatt caattatctt cttccagaaa aagatcgaca cctaccctct
    25621 tctcctcacc cttaccgact ccgcgtgagc gtggagcccc tccgcgagtt gcagcaggac
    25681 ggtaaagcgc cgacagggtg atatcggagg ccgtagcgcc ggtgccgg
//
LOCUS       DAOSEE010000013        25132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_889_length_25132_cov_226.921003, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000013 DAOSEE010000000
VERSION     DAOSEE010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 25132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25132
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_889_length_25132_cov_226.921003"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..609
                     /locus_tag="PLI76_01945"
     CDS             133..609
                     /locus_tag="PLI76_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HOI60823.1"
                     /translation="MKETIDGIKIKKLNVIPDERGWLMEILRCDDEIFERFGQVYCTT
                     AYPGVVKAWHYHKKQTDNFTCVHGMMKVALYDARKDSPTYKGLMEFFVGEKNPILISV
                     PPGIYHGFKAIGTETAFFVSIPTLPYNYKEPDEFRLPPDTVEIPYDWGLTPGLKHG"
     gene            622..1587
                     /gene="rfbB"
                     /locus_tag="PLI76_01950"
     CDS             622..1587
                     /gene="rfbB"
                     /locus_tag="PLI76_01950"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOI60824.1"
                     /translation="MTMLVTGGAGFIGSNFIRHMLTEHPDIPIINLDALTYAGNPESL
                     RGVAENPNYTLIKGDICDTTTVAAIFQNHPIDTVIHFAAESHVDRSISDGSAFVRTNV
                     LGTFTLLDCALKHRIRRFIHISTDEVYGSIFEGSFVETDNLNPSSPYSSSKAGSDLLA
                     RSFFITHGLPVIVTRCTNNYGPYQFPEKLIPLFVTNLLEGKKVPIYGTGRNIRDWLYV
                     IDHCRAIDFVLQHGKSGEVYNIGGGAEKTNFEITTKILELLGKDESMIDYVPDRKGHD
                     FRYSLDFGKLRTLGWKPAYTFDDALAATVTWYVENEWWWRPLKIR"
     gene            1621..2526
                     /locus_tag="PLI76_01955"
     CDS             1621..2526
                     /locus_tag="PLI76_01955"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOI60825.1"
                     /translation="MKYYQWLLGRILNKSFGLWERLGIHIVPNHFYQPIPDVGHLDDR
                     LWETPSALIGIDKGEQSQVALLSRLQEGYKPEYDQFPASKTDIPYEYFVNNGGFESVD
                     GEILYCIIRYFKPRRIIEIGSGNSTYLSALAIQKNKEEDREYTCELTAIEPYPNKVLR
                     AGFPNLSRLIKEPVQNIPLSVFERLGENDILFIDSSHVLKAGSDVQYEYNEILPRLNK
                     GVLIHIHDIFLPFEYPKKWICQHHLFWTEQYLLQAFLTYNPTIKVIWAGYYMHRNHPE
                     LLEKAFKTYNRERIEPGSFWMRKVL"
     gene            2551..4143
                     /gene="rfbD"
                     /locus_tag="PLI76_01960"
     CDS             2551..4143
                     /gene="rfbD"
                     /locus_tag="PLI76_01960"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOI60826.1"
                     /translation="MAEEIILIFGANGMLGRDLATVFPGARLCGHKDLDVTDEVAVRT
                     YILSIKPDLVINAAAYTNVDRCESDPGTAFAVNGDAPGYIAAACREAGARLIHYSTDY
                     VFDGKKTEYIESDEPNPINVYGASKLRGEQNIVKIMDDYRIIRTSWLFGRYGRNFVET
                     IRQLSQTNDTVRVVTDQVGKPTYTVDLARKTVEIIECPPGVYHVTNDGACSWYEFARA
                     FASNVVPCTSDEFPRNAKRPAYSVLVSTKTSPMRPWKEALKDYLRSTVRSPMKGIILA
                     GGTGSRLYPLTKVTNKHLLPVYDKPMIYYPLQTLVAAGIEDIMIVSGRGHVGHFLELL
                     GSGKEFGVRLTYEIQEDAGGIAQALGLAESWTGTDNIAVILGDNIFQDDIRKDVESFE
                     SGAKIFLKEVPDAQRFGVAEIRGSKVLGIEEKPRAPKSNLAVTGLYLYDSGVFDIIKT
                     LKPSGRGELEITDVNNAYIQRGAMEFTVLNGFWSDAGTFESLLRASVMVQNHGVRWSV
                     AGKSSLRSIVDPPKIVDGEKMD"
     gene            4196..4888
                     /locus_tag="PLI76_01965"
     CDS             4196..4888
                     /locus_tag="PLI76_01965"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOI60827.1"
                     /translation="MSKNELYQDKGDSYFSDTRWDIISLIPDGNHRILEVGCGTGNTL
                     IKLRELKKAREIIGIELNYDVVQDNCNQLDAILVGDVEEIEPTFEESYFDYIIFADVL
                     EHLVNPAAILERYSLYLKDTGYIIASIPNIKNYRILLDLVLYDKFEYVDAGILDRSHL
                     RFFTRKEVYRLFDQVGLGVVSVQSKFGGGHLGNIDRRLNYSLGRYLPGKSFLSVQYLI
                     TARKRNVKENSY"
     gene            5185..6303
                     /locus_tag="PLI76_01970"
     CDS             5185..6303
                     /locus_tag="PLI76_01970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOI60828.1"
                     /translation="MPRVAIIILNWNGWADTIQCIDSIHLLSNSDYDVILVDNHSSDS
                     SIENIRRYCEGTGDKKLIGNAPIHLFELLEDDVDSYALDLSSYNHLEPCRRLILIKNN
                     ENHGYARGNNIGIRFALNALKSKYILILNNDVIIKDKEMLGKLTGAINRDSRIGAVSP
                     ILRSTAGEIQRTCTGNLPDFLDFIFVYTFIGQRLFKESKVLKKHFNYDYPFDRPREFG
                     VLGGSCILFRSQALEGVGLFDENTFLYWEEYIIGCKFARHGWKLLLNPDTEVIHKGES
                     CIKNMNLKSWARYWSIKSELYYIENYASMNAIKRSIIRFTLLLETILALFDTILKGGE
                     SKFDREYELKSISILLGYPAKTGASWQQDTPEQHGRSF"
     gene            complement(6541..7257)
                     /locus_tag="PLI76_01975"
     CDS             complement(6541..7257)
                     /locus_tag="PLI76_01975"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOI60829.1"
                     /translation="MNIRFRERAGVFVMQAGQMLGVGMVRIPFGVLRGKRWQFVSSIP
                     SCALGTYEQHIATVLQSRLRDGTTFFDIGANVGYYTLFASTLVGDSGRVISFEPEPEN
                     MRMLVVHTTINKCTNVTPVRKALSDMPGIVRFDGTAHTMCKFSDDGNIEVECTTLDAF
                     IQDSGISPDILKMDVEGAEVRALRGADACLSEIRPEIILSVHDGLLDKCRAILSAYDY
                     SIEQLSPDDLYCVPLERRAR"
     gene            complement(7254..8357)
                     /gene="wecB"
                     /locus_tag="PLI76_01980"
     CDS             complement(7254..8357)
                     /gene="wecB"
                     /locus_tag="PLI76_01980"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOI60830.1"
                     /translation="MSVAIILGTRPEIIKMSPIIRECERRGDDYFILHTGQHYSYSMD
                     RIFFEQLRLPDARYNLNVGSGLQGEQTGKMLGEIEQVLIDEKPHAVLVQGDTNTVLAG
                     ALAASKLHIPVGHVEAGLRSNDRRMPEEINRILTDHISDYLFAPTVESRKNLVYEGIP
                     DEKVFVCGNTVVDALFQNLSISEEAIDPLSELGVRPDGYFLVTAHRQENVDDARKLGD
                     ILEGLERIADEYAMPAIYPMHPRARKMMERFGLKTEAVCCVEPLDYLSFLQLEKHAGL
                     ILTDSGGVQEEACILRVPCVTLRENTERPETVAAGANILVGTNPEMIVEGVRVMMERE
                     RTWANPYGDGATGRRIVEILDSGRCCASGDGST"
     gene            8508..9677
                     /locus_tag="PLI76_01985"
     CDS             8508..9677
                     /locus_tag="PLI76_01985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOI60831.1"
                     /translation="MGLKICILSVDFLPNVGGIAAHVYELARALQNLGNEVTIVTFRD
                     ALFAPKEETIDGLHVVRIYLPRNTVLLYPAFALFEYFSVRRIIKAKKIDLLHSHYVFP
                     DGFVSRLQPGVPGIATEHTSGFLDRLEQGKMLGLYRWVYARMDHIITPSDELAEAVGS
                     LGIPRQKITFVSNGVDTEKFAPEALPKNLRQQYGLAPETRIVLCPRRLEPKNGIKYLV
                     EAVPVVLRKCPDTRFFIVGGSYPDQLALLQKRAFELDVQDHITFVGSVPNAEMVSWYT
                     AADVVVLPSLKEATSIAGLEAMACGRPLVGTSVGGIPYLIDDGKTGLLVEPRDPRGLA
                     DALVHILTDDAARREMGATARKKAVDRFSWTRIAGIVHSIYKKNLRDVENGDRRR"
     gene            9658..10446
                     /locus_tag="PLI76_01990"
     CDS             9658..10446
                     /locus_tag="PLI76_01990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOI60832.1"
                     /translation="MGIGDGDGMGISACLITKNEEEALARSLKMISAYVDEIVIVDGQ
                     SEDKTVEVAREFDAVVIQREFSGSFAIERNSGIERARNEWVFILDPDETLEPGFLQTL
                     RDLATSRKYDAYSFLRHDVLPDGTILETPCGHPEIHVRLAKKDRLRYYGAIHEKAVVS
                     GKIKFIPEAIYHHRGYFEDYPAEKKERFEAIGRNASEDLQGLKISSHTLLARNVKLVF
                     HYFRNMLIGMGLYRKGPSGIIQAVRYTYSFTSYGLKQYVRLGQI"
     gene            complement(10480..12303)
                     /gene="asnB"
                     /locus_tag="PLI76_01995"
     CDS             complement(10480..12303)
                     /gene="asnB"
                     /locus_tag="PLI76_01995"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012108019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOI60833.1"
                     /translation="MCGIAGQFNLSGEAVDRDLIRRMSEFLVHRGPDGDGIHIDGMIG
                     LAHRRLAIIDLSEEGCQPMTTEDGTLWLVFNGEIYNHRELMEELISCGHRFRSRTDTE
                     VILHAYEEWGRDCLARFNGMWAFALWDSQRKELFCARDRLGIKPFYYALAGNSFLFAS
                     EIKALRVHPEIGRRPDDEMVAAFLAWGLADHTDRTMFEGVLQLLPAHYMVIRKGGIAE
                     HAAFWEVTFNPALESRPDSDECLASQFLSLLEDAVDLHLRSDVPVGTCLSGGLDSSTL
                     TVLINRALHRGSPETEPPRQDTFSACFEDERFDESTYVDIVVESSGVRSHRVYPATDT
                     IWADIEHLLYINDEPFAALTLYSQYCVMREAQREVKVVLDGQGADELLAGYIAYQFCH
                     IRGLSRKFRILQAARESFGLLRHHRDFLRYGLTQSFARKSRRSHLCLEKPLFPRYHGT
                     LGEALETDLMRANLPYLLHWEDRTSMAFSLEARVPYLDYRIVEFLASLPADQKIRNGV
                     TKFILRKAIRGLVPDAIRCRMDKKGFATPEEVWMQGDMSEKVLEILSSETFRNRPYWS
                     ADAIEKSYRDFLVGKATYSSEIWRMVCCELWLRRFIDPVAS"
     gene            complement(12462..13583)
                     /locus_tag="PLI76_02000"
     CDS             complement(12462..13583)
                     /locus_tag="PLI76_02000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOI60834.1"
                     /translation="MLLWIGIGLIIAAVAPYVVYFIGITIGRKPDKLQILNEYPAISI
                     IISAYNEEAVIRERVWNILASSYPVDRYEVIFIDDCSSDNTRRIAEETFAEAGITHRI
                     IANAERLGTNRSYNKVMSLARHPIIVTTDADIFFERDALAHLIARLISDDRIAAVCGD
                     LHPLPGDPSRPAQMESAYRNYYGRMCTWESAVDSTYTFNGALVAFKRDLVTRIDDKRG
                     ADDANTAFEAIRRGYRTAYEPAAVVYEDIPPNFSGQYRQKIRRATRLIEATTANFDLL
                     GNSRPFSRYFYPLRIYMYLVTPGLFFIGSALFAVGLALANPLLATALFSVFFLIIILW
                     RNSTAVSFVTNQVYLAKGLLNLGKDMRVWESTSKKAGTG"
     gene            complement(13876..15204)
                     /locus_tag="PLI76_02005"
     CDS             complement(13876..15204)
                     /locus_tag="PLI76_02005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOI60835.1"
                     /translation="MQNLLVKITDRSAKIGIIGQGYVGLPLAMAFAKEFAVFGYDVCS
                     NTIEQLRAGKSHIQDVSDTQLSRHLGLTYSPTSDPEDLHQCDFLIICVPTPLSEDKIP
                     DLSCIKDACSTLKTVLRRGQFVILESTTFPGTTDEVVVPILEETRLAAGSDFGIAYSP
                     ERVDPGNKRYPIDKVPTVVGGINAECTEIAAALYGSVVDRVIPVQDARTAEAVKMIEN
                     IFRNVNIALVNEMALIFERMGINTWEVIDAAATKPYGFMPFYPGPGIGGHCIPLDPYY
                     MSYRAKKYGFIPRFIETSGEINEFMRMHTVNLVEKGLRRVGKRVYGAKVAVMGLAYKK
                     NINDPRESPSVKIIEELVNLGADVRVYDPFVPSLATKAGVFTSAGSLEEALQGADCAV
                     FLVDHDVFKGLDAGYVGSCMNSPVLVDCKNLFQWRDGIVYLCIGKGERDR"
     gene            complement(15844..16437)
                     /locus_tag="PLI76_02010"
     CDS             complement(15844..16437)
                     /locus_tag="PLI76_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HOI60836.1"
                     /translation="MIEYGRNALGEGATIFEPVTIGFPSRDRMGEEDYPGATIGRNAI
                     LRSGTIIYCDVVIGDAFQTGHNVLIREKTTIGDRVAIGTAAVVEGDCTIGDDVRLQSL
                     VYIPTGARIGNRVFIGPNAVLTNDRYPPGPREGLRGPVIGDDAVIGANATVLPGVVVG
                     EGAFVAAGAVVTKDVPPTMLAVGAPARFRPLPSGAKR"
     gene            complement(16434..18173)
                     /gene="glmS"
                     /locus_tag="PLI76_02015"
     CDS             complement(16434..18173)
                     /gene="glmS"
                     /locus_tag="PLI76_02015"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOI60837.1"
                     /translation="MCGIVGYIGRRDATGVLIQGLKRLEYRGYDSFGLATVGSAIEVY
                     KKVGRISDGEAGATGLAGYAGIGHTRWATHGEPNDINAHPHTDCTGRIALVHNGVIEN
                     YSELKRQLEGRGHVFRSETDTEVIAHLIEEQYDGDLLAAVSAILPRLEGSYAVLVIAA
                     DTQKIVAARNASPLVLGVGDAEVFAASDMTPLLEYTERVVYLEDGDVADITPDRYTIY
                     YDGREVERPIELISWCVEDTRKGGFAHYMLKEIFEQPQSFYETIRAGIDDHVRQMVME
                     ADEITLVACGTSYHTALVFKYLAEPLCNLPIRVEMGSEFKYFTPPLHGLVIAVSQSGE
                     TADTIAALKMAKARNCPTLAITNVQGSTVTRIADETLLMRAGPEIGVAATKSYTAQLA
                     ALMQIVNARCDDAFDDVLSHAHLAISEVLIREIREAVALCAKAEHVFFVGRGPFYPVS
                     MEGALKMKEISYVHAEGYAAGELKHGPFALLTPETPVVAICTPGPTYSVMASNIKEMK
                     ARRAPVIALGVDGDKELAEIVDIFIPIPNTHLLVQILTISVTLQLLAYHTACALQRDV
                     DKPRNLAKSVTVE"
     gene            complement(18174..19325)
                     /locus_tag="PLI76_02020"
     CDS             complement(18174..19325)
                     /locus_tag="PLI76_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOI60838.1"
                     /translation="MQAVILAAGEGSRLRPLTRSKPKSMLPVANRPIIEYVIDALLEN
                     GIRDIIVVVGYRKEDVIRHLNRLDAPIQVVVQERQLGTADALRAAESEITGDFLVLPG
                     DNYVNAESIARIRKERNAMLVAEHPSPSNFGVVVIRNGLVREIIEKPEDAPTFTVSTG
                     IYSFSPDVFSCLRTNEIPDALAAMIAAGHRIRAIPADDWQDAIYPWDLLKLNNRMLRE
                     ISPEVGGTVDASVIRRGTVRIGTGTTVGPNTVIYGPVAIGDNCNIGSNCVIMPNASIG
                     DRVEIEPFTYVADSLIMSDARIGSHSRIVSAVFGQGCILADHTTTYPSASFIEVGERV
                     QKEEFGAVLGDGVRAAPFTTFKNCIAGNNVTIERGKTVVGLIEDGTRVM"
     gene            complement(19330..20529)
                     /locus_tag="PLI76_02025"
     CDS             complement(19330..20529)
                     /locus_tag="PLI76_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOI60839.1"
                     /translation="MQCVVLAAGEGKRMRPLTARRPKVMLPIANRPIMEHLVVAARDA
                     GITDFVFVVGYFEREIRNHFGDGSSLGVKITYVTQRHQLGTADALRATAGMVNGRFLL
                     LNGDMVLKRSDIERFCRMSVPCMGIHETDHPEDYGVVTVEGGRITGLEEKSERPKSNL
                     INAGAYLFDPDIFDLLAGVKISGRGEFELTDALESYIREGTLAAHPLDYWLDVGQPWD
                     LLDANEALLSSLCCEWRGTIEDGCTVPDTVSIGEGTIIRAGTYIEGHCVIGENCVIGP
                     HAYIRGSTAIGDGCHVGHATEIKNSIVMARTNIPHFNYVGDSIVGSGCNFGAGTKVAN
                     LRHDNAAVKVCGKSTGRRKFGAIIGDNVLFGINCSVNVGSLIGSNTRVAPHSLVEGCI
                     EEGSVIR"
     gene            complement(20535..21803)
                     /locus_tag="PLI76_02030"
     CDS             complement(20535..21803)
                     /locus_tag="PLI76_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase/phosphoglucosamine mutase"
                     /protein_id="HOI60840.1"
                     /translation="MLFGSSGIRQEFGPGLVDLALRIGAAAADGASPLVVGRDTRTTS
                     ELLEHCVVAGMLSAGAAVYTCGIAPTPTVAYATRRADAGCMITASHNPEPYNGVKLLN
                     PDGSSFTRRQQAAIEEALEGTHRVGWEEQGHTSPIDALAMHRDAILGEISLSRPVTVV
                     LDCGNGAGSVITPDLLSDAGATVVGLNCNVSGRFARPSEPLEANLPYVGEIVRKRGAG
                     CAVIHDGDADRMMAFDERGRYIDGDHLLMLFATYLDHKRVVTTVDASMAIEEVAEVRR
                     TPVGDTFVSEELLTWGDFGGEASGSWIFPGHSYCPDGVYAAALLCEIASEWSIAEELD
                     RMPRYTLLRESCRVASPGEIMAAIGADEPTHGVRFSDDDGWYLIRASGTEPKIRITAE
                     GKTPEKAKEMLDAGHAALARAKRILGGREE"
     gene            complement(21841..22446)
                     /locus_tag="PLI76_02035"
     CDS             complement(21841..22446)
                     /locus_tag="PLI76_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60841.1"
                     /translation="MSFLNDAIRRLEHTLADSGCENGIVDLSVNYNADVCQYPKGVCM
                     EGHFGGRTGQFVTSEPIRARTRISFMFGAPLTDQKQRGAASAIINAVSAFLCLARKLR
                     PCTPDCYAPCLADLSREIGRMRIYPVGPMPVIERAFKDRIVDSPEEADILLVAGEGMT
                     TDAGIACIDGCRGNKRIIFLGPSTAGVSGLLNLEHWCPYGR"
     gene            complement(22448..23056)
                     /locus_tag="PLI76_02040"
     CDS             complement(22448..23056)
                     /locus_tag="PLI76_02040"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOI60842.1"
                     /translation="MIIYDITRDLSADAILYPGDIRPRFREIDNGQYRVTEMTIGTHS
                     GTHIDAPSHYIEGGLTVDRIALGVLAGSARVIDCSDAETLIGPENLADRLAGARTLLL
                     KTWFSGREEFDPGYPALSPEAADLVVEAGIACIGTDAPSIEAFCGDGSVHRRLLGAGT
                     VVLELLDLAAVPEGEYHMVALPMRLAGADGSPVRAILCNGKE"
     gene            complement(23053..23718)
                     /locus_tag="PLI76_02045"
     CDS             complement(23053..23718)
                     /locus_tag="PLI76_02045"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOI60843.1"
                     /translation="MTKTDPYEREREAMVEHQIRDRGIRDERVLAAMREVPRHLFVPK
                     GYERAAYEDRPLPIGEGQTISQPYIVAVMTEQLEIKPQDRVLEIGSGSGYQAALLAGL
                     AGTVISIERLEGVAEQARENLARAGVTGVRIVVGDGTEGYPPEAPYDAIIVTAASPGV
                     PEPLIEQLAEGGRLIAPIGPRDCQDLVKLVKREGKVETIPLGGVCFVPLIGQFGWRGE
                     GFR"
     gene            complement(23737..24828)
                     /locus_tag="PLI76_02050"
     CDS             complement(23737..24828)
                     /locus_tag="PLI76_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOI60844.1"
                     /translation="MERLSQEDRERLALTGLEVGMQNRCGSFFQIDQKVIQTTCGAEG
                     IEMLPIRAALEKYDWVKDYYWNAVSKDKDKYTQFVAKQENPQGLVVIAHEGAKVAMPL
                     QACLFLRDEPVQHVHNIFIAREGSEMHIISGCASSWQKEHGAHIGVTEIYVDKGAKVT
                     STMIHNWNPGISVFPRSATIIEEGGVFLSNYVCMQPVNRVDMYPTARLVGKNAVARFS
                     SIVVATPGSILDLGSRAILDAEQTSAELVTRAITTGGKVISRGHILGEKDGTRGHIEC
                     KGLILKDGIIHAIPEIEGTLTGTELSHEAAVGKIARHEIEYLMARGLSEEEATATIIR
                     GFLDVKISGLPEVLQKQIDAAIDKAESGF"
     gene            complement(24834..>25132)
                     /locus_tag="PLI76_02055"
     CDS             complement(24834..>25132)
                     /locus_tag="PLI76_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI60845.1"
                     /translation="VQQPDFLMLDEPESGVDLENMNLMGKEIAGLLEKDVHIVNRRRS
                     GLIITHTGYILDYLEADQGHVMISGRIRCHGNPREILRVVKEKGYGECLRCKQI"
BASE COUNT         5305 a         7339 c         7153 g         5335 t
ORIGIN      
        1 cggcattcct ccccgcacct gttggccggg gtctcctgcc tatatgaaga tgaacccgtt
       61 gcggatctcc cgggatgctg atgagtctgg ctccagagct ccggttggtt atcgcgaaac
      121 tcggtgaaga ccatgaaaga aactattgat ggcatcaaga tcaagaaact caacgtcatt
      181 cccgacgagc gaggctggtt gatggagatc ctccgctgcg acgatgagat ctttgaacgg
      241 ttcggccagg tctactgcac gacggcctat ccgggcgtcg tcaaggcatg gcactaccac
      301 aagaaacaga ccgataactt cacctgcgtc cacggaatga tgaaggtcgc gctctacgat
      361 gcccgcaaag actctcccac ctacaaaggt ctcatggagt tctttgtcgg cgagaagaat
      421 ccgatcctca tcagcgttcc cccggggatt taccacgggt tcaaagccat cggcaccgag
      481 accgcattct tcgtgagcat cccgactctg ccctacaact acaaggagcc cgacgagttc
      541 cggctgcccc cggatacggt cgagatcccc tacgactggg ggctcacacc cgggctgaaa
      601 cacggatgaa gggtagcaga aatgaccatg ctcgtcaccg gcggtgcggg attcatcggg
      661 agcaacttca tccgccacat gctcacggag caccctgata tcccgatcat caacctcgac
      721 gcactgacct atgcgggaaa ccccgagagc ctccggggtg ttgctgagaa cccgaactac
      781 accctcatca agggtgacat ctgcgacacc actacagtcg cggctatttt ccagaaccac
      841 ccaatcgata ccgtcatcca tttcgctgca gagagccacg tggaccgctc gatctcagac
      901 ggttccgcct ttgtccgaac gaatgttctt ggaaccttca ccctgctcga ctgtgcgcta
      961 aagcaccgga tccggcgatt catccatatc tcaaccgatg aggtctacgg gagcattttc
     1021 gagggctcat ttgtggagac cgataacctg aacccttcaa gcccctactc gtcgagcaaa
     1081 gccggttctg atctgcttgc ccgctcgttc ttcatcaccc acggcctccc ggtgatcgtc
     1141 acccgatgca ctaacaacta cggcccctac caattccccg agaagttaat cccgctcttt
     1201 gtcacgaacc tcctcgaggg aaagaaagtc ccgatctacg gcaccggcag gaacatccgc
     1261 gactggctct acgtcatcga tcactgccgg gccatcgact tcgtcctcca gcacgggaag
     1321 tcgggggagg tctacaacat cggtggcggc gcagagaaga cgaacttcga gatcaccact
     1381 aagatcctcg aacttctcgg gaaggatgaa tcgatgatcg attacgtgcc ggaccggaag
     1441 gggcacgact tccgctattc gctggacttc gggaaactcc gcacactcgg gtggaaaccg
     1501 gcgtatacct tcgatgatgc cctcgcggca acggtcacat ggtacgtgga gaatgagtgg
     1561 tggtggcgac cgctgaaaat cagataagat ggttacgggg gtggttttat agtagaattg
     1621 atgaaatatt accagtggtt gctcggtcgc attctgaaca agtcattcgg gctgtgggaa
     1681 cgattaggaa ttcatattgt gccaaatcac ttttatcagc ccatacctga tgtcgggcat
     1741 ctggatgatc ggttatggga aacgccatcg gcattgatcg gtatcgataa aggtgaacag
     1801 tcccaagttg cattgctctc tcgactccag gagggctaca aaccagaata cgaccaattc
     1861 ccagccagca aaaccgacat accgtacgaa tatttcgtga ataatggagg ttttgagagt
     1921 gtcgacggtg agatcttata ctgcatcata cggtatttta agccaagaag aatcattgag
     1981 attgggtccg gaaactctac gtatctatcg gcattggcaa tccagaagaa caaagaagag
     2041 gatcgggagt acacctgtga gttaactgcc atcgaaccct atccgaataa agtgttgcga
     2101 gctggttttc caaatttgtc ccgcctgatc aaagaacccg tacagaatat cccattatca
     2161 gtctttgaga gactaggtga gaacgatatt ctctttatcg attcaagtca tgtcttaaag
     2221 gcaggcagcg acgtccagta tgaatataat gagattttgc cgagattgaa taaaggtgtg
     2281 ctcatccaca ttcatgacat tttcttgcct tttgaatacc ctaaaaaatg gatctgccaa
     2341 caccatcttt tctggacgga gcagtacctc cttcaggcgt ttttgacata taaccccacg
     2401 atcaaagtaa tctgggccgg ttattacatg cacagaaacc acccggaact cctggaaaaa
     2461 gcgttcaaaa catacaatcg ggagaggatt gaacctggaa gcttctggat gaggaaggta
     2521 ctatgagtga tgagtgcacg aggaattgag atggcagaag agattattct gatcttcggg
     2581 gccaatggga tgctcgggcg tgacctcgca acggtcttcc ccggcgcccg gctctgcggg
     2641 cataaagatc tcgatgttac tgatgaggtt gcagtacgaa cgtatatcct ttctattaaa
     2701 cccgacctcg tcatcaacgc cgccgcatac acgaatgttg acagatgcga aagtgacccg
     2761 gggacggcgt ttgccgtcaa cggtgacgcg ccggggtaca tcgccgccgc ctgccgcgag
     2821 gcaggtgccc gactgattca ctacagtacc gactatgtct tcgacggcaa aaaaacggag
     2881 tacatcgagt ctgatgaacc gaaccccatc aacgtctacg gcgcctcgaa actccgtggc
     2941 gagcagaata tcgtaaagat catggatgac taccggatca tccggacttc ctggctcttt
     3001 ggccgatacg ggaggaactt cgtcgaaacg atccggcagc tttcgcagac gaacgatact
     3061 gtccgggtgg tgaccgacca ggtgggcaag cccacctaca cggtggatct cgcccgaaag
     3121 acggttgaga tcatcgagtg cccgcccggg gtctatcatg tgacgaacga cggagcctgt
     3181 tcgtggtacg agttcgcccg ggccttcgcc tcgaacgtgg tcccctgcac aagcgacgaa
     3241 ttcccacgaa acgcgaagcg cccggcctac tcggtgctcg tgagcacgaa aacgagcccc
     3301 atgcgcccct ggaaggaggc gctgaaagac tacctgcgct ccacggtgag aagtcccatg
     3361 aaaggtatca tacttgcagg tgggaccggg tcccgcctct accccctgac gaaagtgacg
     3421 aataagcacc tgctcccggt ctacgataag ccgatgatct actaccccct ccagacactc
     3481 gtcgccgcag ggatcgagga tatcatgatc gtctccgggc gagggcacgt gggccacttc
     3541 ctcgaactgc tgggctcggg aaaggagttc ggggtccgac tcacctacga gatccaggaa
     3601 gacgccggcg ggatcgccca ggccctgggg cttgcggaat cctggaccgg gacggataac
     3661 atcgcagtga tcctcggtga caacatcttc caggacgata tccggaagga cgttgaatcg
     3721 ttcgagtccg gggcgaagat cttcctaaaa gaggttcccg acgcccagcg attcggggtc
     3781 gccgagatca gagggagtaa agtactcggg atcgaggaga aaccccgggc cccaaagtcg
     3841 aatctcgcag ttaccgggct ctacctctac gattccgggg tctttgatat catcaaaaca
     3901 ctcaagccat ccggacgggg cgagcttgag atcacggatg tgaacaacgc ctatatccag
     3961 cgaggcgcga tggaatttac agtactgaac ggattctgga gcgatgctgg aacatttgaa
     4021 agtctgttgc gggcgagtgt gatggttcag aaccatggtg tccggtggag tgttgccggg
     4081 aagtccagtc tcagatcgat agtcgatccc ccaaagattg ttgatgggga gaagatggac
     4141 tgaaccgagc ctcccggcag gatagactct aaagatagcg ttatccggcg agatcatgtc
     4201 gaaaaatgaa ctgtatcaag ataaaggtga tagctatttc tccgacacac gatgggacat
     4261 catctcctta atccctgatg ggaatcatag aatactggaa gtcggttgcg gaaccggaaa
     4321 tacgctgatc aagctgcgag aacttaagaa agccagagaa attatcggga tagagttaaa
     4381 ctacgacgtc gtgcaggaca attgcaacca gcttgatgcc attctggttg gcgatgtgga
     4441 ggagatcgaa ccgacctttg aagaaagtta ctttgattat atcatctttg ctgatgttct
     4501 tgagcatctt gtaaaccctg cagccattct agagcggtac tccctgtatc ttaaggatac
     4561 gggatatata atcgccagta ttccaaacat taagaactac agaatactgc ttgatctggt
     4621 gctgtacgat aagtttgagt atgtagatgc aggaatcctg gatcgctcgc acctccgctt
     4681 tttcacgcgg aaggaggtgt accgcctgtt cgatcaggtc ggtctgggcg ttgtctcagt
     4741 gcaatcaaag ttcgggggtg ggcacctcgg caacatcgat agacgcctga attactcttt
     4801 gggcagatac ctgccgggaa aatctttctt atctgtccag tatctcatca cggcaagaaa
     4861 gagaaatgtg aaagagaatt cctattaacc ctcactcgca tccggccctt gagtcccctg
     4921 ctgcccttca gtagtgctcc atcatatagt tgctggctgg ctttagaggg caacaccctg
     4981 tgcttatgat agaaaaccca tacagtggga attctccccc cattatcagc tgcttcttat
     5041 agcattaccc ggcaattgag tctgcggtcg agtgcagctt tacgaatatt ttatgtaaca
     5101 agtccttaat tgtgccggaa taggtatttt ccagtataat atcaaaacct ataacggcag
     5161 gtgcaatcta tataaatagc gataatgcct cgcgttgcga ttataatttt aaattggaac
     5221 ggatgggcag atactattca gtgtatcgac tccattcatc tgctctctaa ctcagactac
     5281 gatgtcatat tggttgataa tcactcaagt gacagctcga tagagaatat ccggcgttat
     5341 tgtgagggaa caggggataa aaaacttatt gggaacgctc ctattcacct cttcgagttg
     5401 cttgaggatg acgtagacag ttatgcactc gacctttcgt catacaacca cctggagcct
     5461 tgccggcggt tgatactgat aaaaaataac gagaaccatg gttacgccag aggcaataac
     5521 atcggcatca gattcgcact taacgccctt aaatcgaaat atatccttat tctcaataac
     5581 gatgtaatca ttaaggataa agaaatgctc ggtaagctca ccggagcgat taaccgggac
     5641 agccgtatag gagccgtgag cccgatactc cggtcaacgg ccggtgagat acagagaacc
     5701 tgtacgggaa atcttcccga cttccttgat ttcatatttg tctacacgtt catcggtcag
     5761 agactattta aagagagcaa agttctgaag aagcatttta attacgatta ccctttcgat
     5821 cgaccgagag agttcggagt tttaggaggt tcctgcatcc tcttcagaag ccaggccctc
     5881 gaaggtgtcg gtctcttcga tgagaacacg ttcctttact gggaagagta cataatcggg
     5941 tgtaaattcg ccagacacgg ctggaagttg ctcttgaatc cggatactga ggtcatccac
     6001 aagggtgaat catgcataaa gaatatgaac ctgaaaagct gggcaaggta ctggtcgatc
     6061 aagagtgaac tctactacat cgagaactac gcatcgatga atgccatcaa aagatccatt
     6121 atcaggttta ctctgctctt agagacgatt ctagctctat ttgatacgat cttaaaagga
     6181 ggggagagta aatttgacag agaatatgag ctgaagagca tctctatctt attgggatac
     6241 cccgctaaaa cgggtgcatc ctggcaacag gatactccgg aacagcacgg aagaagcttt
     6301 tgagcaacgg ggcaggcggt tagatatatc acaataactc ccttttaact cgcgggagtg
     6361 agggggcgga gcggggaatc ccgcccaaaa agcgcggggt agttcgctac cagaaggcct
     6421 gggatattgc tatctcagat cttctgtatt ctcccgcaac atcgcgcagg gcacatggag
     6481 aatacatgct tcttcccgta tgctccacac ctcgcctgca tatatcctgc ttacgatgtg
     6541 ctacctcgct ctcctctcca gtggcacaca gtacagatcg tcgggcgata attgctcaat
     6601 gctataatca tatgcggaga ggatggcccg gcacttgtcc agtaggccat catgcaccga
     6661 caatatgatc tccggtctga tctctgagag gcaggcatca gcgccgcgca gtgctcttac
     6721 ctctgcccct tcaacgtcca tcttcaagat atcaggtgag attccactgt cctggatgaa
     6781 cgcatcgaga gtggtgcact caacctcgat attaccatcg tcagagaact tgcacattgt
     6841 atgtgcagtt ccatcgaatc gaacgattcc gggcatgtct gataacgctt ttcgtacagg
     6901 ggtaacattg gtgcacttat tgatcgtagt atgcacgaca agcatccgca tgttttctgg
     6961 ctccggttcg aatgaaatca ccctcccgga gtcaccaaca agggtggagg cgaacaaggt
     7021 gtaatatcca acgtttgcac cgatatcaaa aaatgttgtt ccatccctca atctggattg
     7081 cagtacggtt gcaatgtgct gctcataggt tccgagagca caggacggaa tggaggagac
     7141 gaattgccat ctctttcctc ggagtacgcc aaaggggatc cggaccatgc cgaccccgag
     7201 catctgccct gcctgcatca caaaaacccc agcacgctca cggaacctta tattcatgtg
     7261 gacccgtccc ccgaggcgca gcatctaccc gaatcaagaa tctccacgat cctcctcccc
     7321 gtcgctccat ccccataagg gttcgcccag gtacgctccc tctccatcat gacccgcacg
     7381 ccttccacga tcatctccgg gttggttccc accaggatgt ttgcccctgc agccaccgtc
     7441 tccggccgct ccgtgttctc gcgtaatgtc acacagggaa cgcggaggat gcacgcctcc
     7501 tcctgtaccc ctccggaatc cgtcaggatc agtcctgcgt gcttctcgag ctgcaggaag
     7561 gagaggtagt cgagaggctc aacgcaacag acagcctccg tcttcaaccc aaatcgctcc
     7621 atcatcttcc ttgcccgggg gtgcatgggg tagatcgccg gcatcgcata ctcatccgcg
     7681 atgcgctcaa gaccctcaag gatatcgcca agtttccttg catcgtcgac gttctcctgg
     7741 cggtgggcgg tgacgaggaa gtagccatct ggccggaccc caagttctga aagaggatct
     7801 atcgcctctt cgctgatcga gaggttctgg aagagtgcat ctacgaccgt gttgccgcag
     7861 acaaagacct tctcgtcggg tatgccctca tacaccaggt ttttccgtga ctccacggtc
     7921 ggcgcaaaca ggtaatctga gatatgatcc gtcaggatgc ggttgatctc ctccggcatc
     7981 ctccggtcgt tgctccgcag gccggcctcg acatgcccca ccggaatatg aagtttcgat
     8041 gcggcaagcg ctcccgcgag caccgtgttg gtatccccct gcacgagaac cgcatgaggc
     8101 ttctcatcga tcaagacctg ctctatctct ccgagcatct ttccggtctg ttccccctgc
     8161 agacccgaac ccacgttgag gttatatcgc gcatccggga ggcgcagttg ctcaaagaag
     8221 atcctgtcca tcgaatagga gtaatgctgg ccggtatgaa ggatgaagta gtcatctcct
     8281 ctcctttcgc attccctgat gatcggagac atcttgatga tctcaggccg cgtgccgaga
     8341 atgattgcaa cgctcatggt tcacctgcat ggttggtgcg atagttcatt gatccggtgc
     8401 atatatagcg ctgttgtcac gacgtttctc gccagggtca actgcttttc gattgcgatc
     8461 tgagaaaaca tataacgagg ccggcataaa tgtagttagg ggagcgtatg gggctcaaga
     8521 tctgcatact ttcggtcgat ttcctcccga atgtcggggg aattgcagca cacgtctacg
     8581 agcttgccag agcgctccag aaccttggga acgaagtcac aatagttacg ttccgggatg
     8641 cactcttcgc tccgaaggaa gagacgatcg acggcctcca cgttgtcagg atctacctgc
     8701 cccggaatac cgtgctgctg tatccggcat ttgccctgtt tgagtatttc agcgtacggc
     8761 gcatcatcaa agcgaagaag atagatctcc tgcattcgca ctacgtattt cccgatgggt
     8821 tcgtatcgag gttacaacca ggggtgcccg gcatcgcaac cgagcatacc tccgggttcc
     8881 tggatcgcct ggagcaggga aagatgctgg gactctatcg gtgggtctac gcacgcatgg
     8941 accacatcat caccccgagc gacgaacttg ccgaagccgt cgggtcgctc ggaatccccc
     9001 ggcagaagat cacgttcgta tcgaacgggg tggatacaga gaaatttgcg ccggaagcct
     9061 tacccaaaaa cctccggcag cagtacggcc tcgcgccgga gacccggatc gtgctctgcc
     9121 cccgccgcct cgaaccaaag aacggcatca aatatctggt cgaggccgtc ccggttgtcc
     9181 tccggaagtg cccggatacc cggttcttta tcgtcggggg atcgtatccc gaccagctgg
     9241 ctctgctcca gaagagggcc tttgaactcg atgtgcagga ccacatcacc tttgtcggat
     9301 cggtcccgaa cgccgagatg gtatcgtggt atacggcggc ggatgtcgtg gtcctgccct
     9361 ccctcaagga ggcgaccagc attgcagggc tcgaagcaat ggcatgcggc aggcctctgg
     9421 tcgggacaag tgtcggaggc ataccatatc tcatcgacga cggcaaaacc ggactccttg
     9481 tggaaccgag agacccccgc gggctcgccg atgcactggt gcatatcctg accgacgatg
     9541 ccgcgcggag agagatgggg gctacggccc gcaagaaagc agtggaccgg ttctcgtgga
     9601 cccggattgc cggcattgtg catagcatct ataagaaaaa tctccgggat gttgagaatg
     9661 gggataggag acggtgacgg catggggatc tcagcctgcc tcataacaaa gaacgaagaa
     9721 gaggctctgg cccgatcgct aaagatgatc tcggcgtacg ttgatgagat cgtaatcgtt
     9781 gatggccaga gcgaggataa aacggttgag gttgctagag agttcgatgc cgtggtaatc
     9841 cagagagagt tctccggtag ttttgctatt gagcggaact ccggtatcga acgggcgcgg
     9901 aatgaatggg tcttcatcct cgatcccgac gagactctcg aaccgggatt cctgcagacc
     9961 ctccgcgatc ttgcaacctc acggaagtac gacgcctact cgttcctcag gcacgatgtc
    10021 ctccctgacg gcacaattct tgaaacaccg tgcgggcacc cggaaatcca cgtcagactt
    10081 gcgaagaagg ataggctgcg gtactatggc gcaatccacg agaaagcggt cgtcagcggc
    10141 aagatcaagt tcattccgga ggcgatctac caccatcggg gctactttga ggattatcct
    10201 gcggaaaaga aggaacgttt cgaggcgatc ggacggaatg cgtcagaaga tctccagggc
    10261 ttgaaaatat cctcacatac actgctcgca agaaacgtga aactggtctt tcactatttc
    10321 agaaatatgc tcatcgggat gggactctac aggaagggtc catccggcat aattcaggcg
    10381 gtacgatata cctacagttt tacgagttac ggattgaagc agtacgtccg cctgggccag
    10441 atctgagggg aaaagagacc tcttcttcct tgcccgggat tacgatgcca ccggatcgat
    10501 gaatcggcgc aaccatagtt cgcagcagac catacgccag atctcagatg aataggttgc
    10561 tttccccaca agaaagtccc ggtaactttt ctctatcgca tcggcactcc agtacggccg
    10621 attccggaat gtctccgacg agaggatctc aaggactttc tcactcatat cgccctgcat
    10681 ccatacctcc tccggcgtgg caaacccttt cttgtccatc ctgcaccgga tcgcgtcggg
    10741 caccaatccc cggatcgcct tcctgagaat gaacttggtc acaccgttcc ggatcttctg
    10801 atctgcaggc agcgaagcca ggaactccac gatcctgtaa tccagataag gtacccgtgc
    10861 ttcgagagag aaagccatcg aagtccggtc ttcccagtgg agcagatacg gcaggttcgc
    10921 ccgcatcagg tcagtctcca gggcttcgcc cagggtgccg tggtaacgag gaaaaagtgg
    10981 tttctccaaa caaaggtggc tcctgcgact cttcctggca aacgactgcg tcaagccata
    11041 cctgagaaag tcccggtgat gcctgagcaa tccgaaggac tcgcgtgcag cctgaagaat
    11101 gcggaacttc cggctcagcc ccctgatgtg gcagaactgg taggcgatgt agcctgcgag
    11161 gagctcatcc gccccctgtc cgtccagcac caccttcacc tcccgctgcg cctcccgcat
    11221 aacacagtac tgggaataca gcgtcagggc ggcaaacggt tcgtcgttga tgtagagcag
    11281 gtgctcgata tccgcccaga ttgtatcggt ggccgggtag acccggtgcg acctgacacc
    11341 cgaactctcc acaacgatat cgacataggt gctctcgtcg aaccgctcat cctcaaaaca
    11401 ggccgaaaac gtatcctggc gcgggggctc cgtctccgga gaaccccggt ggagtgcccg
    11461 gttgatgagc accgtcagag tggatgaatc cagcccaccg gagaggcagg ttccaaccgg
    11521 cacgtcgctt cgcaggtgga ggtcaaccgc atcctcaagg aggctcagga actgcgaggc
    11581 gaggcactcg tctgaatcgg gcctagactc cagtgcaggg ttaaacgtaa cctcccagaa
    11641 ggctgcatgc tctgcgatcc cacccttcct gatgaccatg tagtgcgccg ggagcagctg
    11701 gagcacccct tcaaacatcg tccggtcggt atgatctgca agaccccacg cgagaaacgc
    11761 ggccaccatt tcatcatctg gccgtctgcc tatctcagga tgtactcgta gcgccttgat
    11821 ctcggaggca aagaggaacg agttgccggc cagggcgtaa taaaacggtt ttatccccag
    11881 gcgatcgcgg gcacagaaca gttctttcct ctgtgaatcc cagagggcaa acgcccacat
    11941 cccgttgaac cgggcgaggc aatctctccc ccactcctcg tacgcatgaa ggatcacctc
    12001 ggtgtcggtc ctggaccgaa agcggtgccc gcacgagatc agttcctcca tgagttcccg
    12061 atggttgtag atctctccgt taaagaccag ccagagcgtt ccgtcttcgg tggtcattgg
    12121 ctggcatccc tcctcggaga gatcgatgat tgccaggcgg cgatgggcaa ggcctatcat
    12181 cccgtcgatg tggatgccat ccccatcggg cccacggtgc acgagaaact ccgacatccg
    12241 ccggatcaaa tcccgatcta ccgcctctcc cgagagatta aactgcccgg caataccgca
    12301 catgcttatt caatccgtct cgtagggaga tatattgccc gcgcagtgac tcagcagaga
    12361 taccgttgcc cgccgcccta cgtcttcagt cggggatagg aaaacaggcc ctgccatcca
    12421 gaaccgccga tcccggcccc ggagccgtaa aggcagggct ttcatccggt tcctgctttt
    12481 tttgaggtgc tctcccatac ccgcatatct tttccgagat tgagcaaccc tttagcgaga
    12541 tagacctggt tggtgacgaa cgagacggct gtactattcc tccacaggat gattatcagg
    12601 aagaagacgc tgaagagcgc ggtggcgagg agggggtttg ccagggcgag gccgaccgcg
    12661 aagagggcac tgccgatgaa gaagaggccg ggcgtcacga gatacatata aatgcggagc
    12721 gggtagaaat agcgtgagaa tggacgggaa tttccgagca gatcgaaatt tgctgttgtc
    12781 gcctcgatca gtcgggttgc gcgccggatc ttctgccgat actgcccgga gaagttcgga
    12841 gggatatctt cgtacactac ggctgcaggt tcgtaggctg tccgataccc ccgcctgata
    12901 gcctcaaacg ccgtattcgc atcgtctgcc ccccgtttat cgtcgatccg ggttacgagg
    12961 tcgcgcttga acgcaacaag cgcaccgttg aacgtataag tcgagtctac cgcgctctcc
    13021 caggtgcaca tccgcccata ataatttctg tatgcgctct ccatctgtgc cgggcgcgac
    13081 ggatcgccgg gaagaggatg gaggtctccg cagaccgcag cgatccgatc gtcgctgatc
    13141 aggcgggcaa taagatgtgc gagagcgtcg cgctcgaaga agatatcggc atccgtggtg
    13201 acgatgatcg ggtggcgggc cagactcatt actttgttgt acgagcgatt cgttcccagg
    13261 cgttcggcgt tagcgataat gcggtgggtg atccccgcct ccgcgaaggt ctcttctgcg
    13321 atccgccttg tgttatcgga tgagcagtcg tcgataaaga tcacctcgta tcggtctacg
    13381 gggtaggaag aggcaaggat attccaaacc cgttccctga tcactgcctc ttcgttgtat
    13441 gcggagatga tgatgctgat agcaggatat tcgttgagga tttgcagctt atccggtttc
    13501 cgccctatgg taatcccgat aaagtatacc acatatgggg cgactgcagc aataatgagc
    13561 cctatgccga tccacaagag catattatca atttaatggt ttactattta ttatttagca
    13621 ggtatgatga ggatacctcc ttttcctttc caccctatca aaaatccgcc cggggaagcc
    13681 gatttgtatc aggtgggcgg atgctcaaaa caggtaagcc tatggagccc tgtgattcca
    13741 gacccacaag tcaccacagg gcatgggttt gatatctctt tgtgcggcta tcccatcaaa
    13801 cctcactccg ggaaaagatc gcgcaggcac ggagcctgca ttcgcctcac aaatgcagac
    13861 gataccggtt gaccgttacc tatctcgctc gcctttgcca atacagaggt atacgattcc
    13921 atccctccat tgaaagaggt tcttacaatc tacaagaaca ggggaattca tgcagctccc
    13981 gacgtatccc gcatccagcc ccttaaatac atcatgatcc acgaggaaga ccgcacaatc
    14041 tgccccttga agcgcttctt cgaggctccc cgccgacgta aatacaccgg ccttcgttgc
    14101 aagagagggg acgaacggat cgtagaccct cacatcagcc ccaaggttta caagctcctc
    14161 gatgatcttg acggagggcg actcccgagg gtcgttgatg ttctttttgt aggcgagacc
    14221 catcacggca accttcgcac cgtaaactcg cttaccgacc cgcctgagcc ccttctcgac
    14281 gaggttgacg gtatgcatcc tcatgaactc gttgatctcg ccggaagtct cgatgaaacg
    14341 gggaataaaa ccgtatttct tcgcccggta agacatgtag taagggtcga ggggaataca
    14401 gtgaccaccg ataccgggac cggggtagaa gggcatgaaa ccgtagggtt tggtggccgc
    14461 ggcatcgatc acctcccagg tgttgatgcc catccgctcg aagatgagag ccatctcatt
    14521 gaccagggcg atgttgacgt ttctgaagat gttctcgatc atcttcacgg cctccgccgt
    14581 gcgggcatcc tgaaccggga tgacacgatc cacgacactc ccatagaggg cggcggcgat
    14641 ctcggtacac tccgcattga tgccaccgac gaccgtcggt actttgtcga taggataccg
    14701 cttgttccca ggatctaccc gctccggaga gtaggcgatg ccgaagtcgc tgccggcggc
    14761 gagccgggtc tcttcgagga tcgggaccac cacctcatcg gtcgtccccg gaaaggtcgt
    14821 gctctccagg atgacaaact gcccccggcg cagaacggtc ttgagggtcg agcaggcatc
    14881 cttgatacaa ctcagatccg ggattttgtc ttccgacagc ggtgtcggga cgcagatgat
    14941 gaggaagtcg cattgatgaa ggtcttcggg gtcagaggtc ggggaatagg tcagtccaag
    15001 atgccgggag agctgcgtat ctgaaacatc ctggatgtga gacttacctg cccggagctg
    15061 ctcgatggtg ttcgaacaga cgtcataacc gaagacggca aactcctttg caaaggccat
    15121 cgcaagcggg aggccaacgt agccctgacc gataattcca atttttgcgc tcctgtcagt
    15181 gatctttacg agaaggtttt gcatttgctc ctcctgctgt gctgaagccg gatccaggca
    15241 tgcaccgggc atcaaaccca ttttacacgg caccaaagcg cagtactcgc cccgtaggtc
    15301 agaaccggtc cggtgcatcg gttcggacaa attactctgt ggcatacgag gaataaccgc
    15361 tgcaactcga ttccgggacc tgacaaccgg tagcggatct caccgggcat gtccgatatt
    15421 ttgagtttcc tcgaggcact gtgccgaacc tcccttgccg cggtgtggcc tctgatctcc
    15481 agatatcgtc ctgcggtgct gataatcata tcgcttcggt aaaaccttaa agttattccc
    15541 tttcaaaaaa tcaggtcaat ctcacaatag gacacgggaa aatgatttta tggaccctat
    15601 agtctgtgaa ggagacctgc cgggccgtta gcaaacgtaa caccaggatg cctctcaata
    15661 tttgcccctg cccctatgcc gcctccgtca gggaatcccc cggattgcat cacggatgta
    15721 cgccagggtc tttgctcgcc ccggtcggag ggttccggac cgataccgcg aggtcgccgg
    15781 ataccggata gaatgcgcgc ctgctctcac aagcaggctg ccggccggca ggaatatccc
    15841 gcatcaccgc tttgcccccg agggcagcgg ccggaaccgt gccggcgccc ccaccgcaag
    15901 catcgtgggc gggacatcct tcgtcaccac cgcgcccgcg gcgacgaacg ccccctcgcc
    15961 gacgacaaca ccgggcagga ccgtcgcgtt cgcaccgatg acggcatcgt ccccgatcac
    16021 cggcccgcgg agaccctcgc ggggaccggg cgggtagcgg tcgttcgtca gcacggcgtt
    16081 cgggccgatg aagacccggt taccgatccg ggcgccggtc gggatgtaga ccaggctctg
    16141 gaggcggaca tcgtcgccga tcgtgcagtc cccctcgacc accgccgcgg tccctatcgc
    16201 gacgcggtcg ccgatggtgg tcttctcgcg gatcagcacg ttatggccgg tctggaaggc
    16261 gtcgccgatc acgacgtcgc agtagatgat ggtgcccgac cggaggatcg cgttcctccc
    16321 gatggtggcg cccgggtagt cttcctctcc catccggtcg cgggagggaa atccgatggt
    16381 caccggctca aatatcgtcg ccccctcgcc gagggcgttg cgtccatact caatcattcg
    16441 acagtcacgc tctttgccag gttccggggt ttgtcgacgt cccgctgcag ggcgcacgca
    16501 gtgtggtagg cgagcagctg gagggtgacc gatatggtca ggatctgcac cagaaggtgt
    16561 gtgttcggta tagggatgaa tatgtccacg atctcggcaa gttctttgtc gccgtccacc
    16621 ccgagcgcga tgaccggcgc tctccgcgcc ttcatctcct tgatgttcga ggccatcacg
    16681 ctgtaggtcg gtcccggggt gcatatggcg accaccggtg tctcgggggt gagcagggca
    16741 aacggcccgt gcttgagttc tcccgctgca tacccttcgg catggacgta gctgatctcc
    16801 ttcatcttca gggcgccctc catcgagacc gggtagaacg gccctctccc gacgaagaag
    16861 acgtgctccg ccttcgcaca gagggcgacc gcctccctga tctcgcggat gaggacctcg
    16921 ctgatggcga gatgtgcgtg cgacaggacg tcgtcgaacg catcatcgca gcgtgcgttg
    16981 acgatctgca tcagcgcggc aagctgcgcc gtgtaggatt tggttgcggc gacgccgatc
    17041 tcggggccgg cccgcatcag gagggtctca tccgcgatcc gggtgaccgt gcttccctgc
    17101 acgttggtga tcgcaagcgt ggggcagttg cgggctttcg ccatcttgag ggcggcgatc
    17161 gtatccgcgg tctcccccga ctgcgagacc gcgatcacga ggccgtgaag gggcggggtg
    17221 aagtacttga actcggagcc catctcgacc cggatcggca ggttgcagag cggttcggcc
    17281 aggtacttga agacaagggc ggtgtggtag gatgtcccgc acgcaaccag cgtgatctcg
    17341 tcggcctcca taaccatctg ccgcacgtgg tcgtcgatgc cagcccggat ggtctcgtag
    17401 aaggactggg gctgttcgaa gatctctttc aacatatagt gggcaaaccc gcccttccgg
    17461 gtatcctcga cacaccagct gatcagctcg atcgggcgct ccacctcccg gccgtcgtag
    17521 tagatggtat agcggtcggg tgtgatgtcg gcgacgtcgc cgtcctcgag ataaaccacg
    17581 cgctcggtgt actcgaggag cggcgtcata tccgaggcgg cgaagacctc cgcgtcgccg
    17641 acaccgagga cgagagggct tgcgttcctt gcggcgacga tcttctgcgt atcggctgcg
    17701 atcacgagga cggcatacga accctcgagc cggggaagga tcgcgctgac tgccgcgagg
    17761 aggtccccgt cgtactgctc ctcgatgagg tgggcgatca cctcggtgtc ggtctcggat
    17821 cggaatacgt ggccccgtcc ctcgagctgc cgcttcagtt cgctgtagtt ctcgatgacc
    17881 ccgttatgca ccagggcgat ccttcccgtg cagtcggtat gggggtgggc gttgatatcg
    17941 tttggctccc cgtgagtggc ccaccgggta tgcccgatac cggcgtatcc ggcaagaccg
    18001 gtcgctcccg cttccccgtc ggatatccgg cccaccttct tgtaaacctc gatcgcgctt
    18061 ccgaccgtcg caagcccgaa cgagtcgtag ccacggtact caagccgctt caacccctgg
    18121 atcaggaccc ccgttgcgtc ccgtcttccg atgtagccga caatcccaca catctacatc
    18181 acccgggtgc cgtcctcgat gaggccgacc actgtctttc cccgctcgat ggtaacattg
    18241 ttaccggcaa tacagttttt aaatgttgtg aagggtgccg cgcggacccc gtcaccgagg
    18301 actgcgccga attcctcctt ctggacccgt tctccgacct cgatgaagct tgcggatggg
    18361 taggtggtgg tatgatcggc aaggatgcat ccctggccga agaccgccga gacgatcctc
    18421 gagtgggatc cgatccgcgc gtcgctcatg atgagcgagt cggcaacgta ggtgaacggc
    18481 tcgatctcca cccggtcgcc gatgcttgcg ttcggcatga tgacacagtt tgagccgatg
    18541 ttgcagttgt ctccgatggc gaccgggccg tagatcacgg tgttcggccc gacggtcgtc
    18601 ccggtgccga tacgcaccgt gccccggcgg atgacggacg catcgaccgt cccgcctacc
    18661 tcaggtgaga tttccctgag catccggttg ttcaacttca ggaggtccca gggatagatg
    18721 gcgtcctgcc agtcgtcagc aggtatcgcg cgtatccggt ggcctgccgc gatcatcgcg
    18781 gcaagggcat cggggatctc gtttgtccgg aggcaggaga agacgtcggg cgagaacgag
    18841 tagatcccgg tcgataccgt gaacgtcggg gcatcctcgg gtttctcgat gatctcacgg
    18901 acgaggccgt ttcttatcac cacgaccccg aagttcgagg ggctcgggtg ctcggccacg
    18961 agcatggcgt ttcgttcctt cctgatccgg gcgatcgact ccgcgttgac gtagttgtcg
    19021 cccgggagga cgaggaaatc gcccgtgatc tccgactcgg ccgcccggag ggcatctgcg
    19081 gtcccgagct gccgttcctg cacgacgacc tggatcgggg cgtcgagcct gttgaggtgt
    19141 cggatgacgt cctctttacg atacccgacc accacgatga tgtcgcggat gccgttctcc
    19201 agtagtgcgt cgatgacgta ctcgatgatc ggccggtttg caaccgggag catggatttg
    19261 ggtttgctcc gggtgagcgg ccggaggcgg ctcccctctc ccgctgccag aatgactgcc
    19321 tgcatcatat cacctgatga ctgatccttc ctcgatgcac ccctccacga gggagtgggg
    19381 ggctacccgt gtgttgctgc cgatgagact cccgacgttg accgagcagt tgatcccgaa
    19441 gaggacgtta tcgccgatga tggcgccaaa cttcctccga ccggtcgatt ttccgcagac
    19501 cttcaccgcc gcgttgtcgt gccggaggtt tgcaaccttg gtgccggcgc cgaagttgca
    19561 cccgctcccg acgatgctgt cacccacgta gttgaagtgc gggatgttcg tccttgccat
    19621 gacgatagag tttttgatct cggttgcgtg cccgacgtgg cagccatcgc ctatggcggt
    19681 cgagccacgg atgtaggcat gagggccgat gacgcagttc tcgccgatga cacagtggcc
    19741 ctcgatgtaa gtcccggccc ggatgatggt gccttcaccg atgctgaccg tatcggggac
    19801 ggtgcatccg tcctcgatag tgccgcgcca ttcgcagcat agcgaagaga ggagcgcctc
    19861 gtttgcatcg aggagatccc acggctgccc gacgtcgagc cagtagtcca gcggatgcgc
    19921 ggcaagcgtc ccctcccgga tgtaggattc gagcgcgtcg gtcagctcga actcgccccg
    19981 gcccgatatt ttgactccgg caaggaggtc gaatatgtcg gggtcaaaaa ggtaggcgcc
    20041 cgcgttgatc aggttgctct tcgggcgctc cgacttctct tcaagaccgg tgatgcggcc
    20101 cccttccacg gtcaccacgc cgtagtcctc tgggtgatcc gtctcgtgga tccccatgca
    20161 gggaacgctc atccggcaga acctctcgat atcgctcctc ttgaggacca tatcgccgtt
    20221 gaggaggagg aaccggccgt tgaccatccc cgccgtcgcc cggagggcat ctgcggtccc
    20281 gagctggtgg cgctgggtga cgtaggtgat cttcacgccg aggctgctcc catccccgaa
    20341 gtggttccgg atctcacgct cgaagtagcc gacgacgaag acgaagtcgg tgatcccggc
    20401 atccctggcc gcgacgacca ggtgttccat tatcgggcgg ttggcgatgg gaagcatcac
    20461 cttcgggcgc cgggcggtca gggggcgcat ccgtttcccc tcgcctgcag ccagaacgac
    20521 acattgcata ctactcactc ctctcttcct ccgagaatac gttttgctct cgcaagggcg
    20581 gcatgccccg catcgagcat ctcttttgct ttttccggcg tctttccttc ggccgttatc
    20641 cggatcttcg gttcggtgcc gcttgcccgg atcaggtacc aaccgtcgtc atcactgaac
    20701 cggacgccgt gcgtcggttc gtcggcgccg atcgccgcca tgatctcccc gggtgaggcg
    20761 acgcggcagg actcccggag gagggtgtac cggggcatcc ggtccagctc ctccgcgatc
    20821 gaccattccg atgcgatctc gcagaggagc gccgcggcgt agactccgtc cgggcagtag
    20881 gagtgcccgg gaaagatcca gctccccgac gcctctccac caaaatcacc ccaggtgagg
    20941 agctcttcgg agacgaaggt gtcgccgacc ggtgtgcggc ggacctcggc tacctcctcg
    21001 atcgccatcg aggcgtcgac ggtggtgacc acccgcttgt ggtcgaggta tgtcgcaaag
    21061 agcatgagga gatgatcccc gtcgatatag cggccccgtt cgtcaaacgc catcatccgg
    21121 tcggcgtcgc cgtcgtggat caccgcgcac ccggcccccc gcttccggac gatctcgccg
    21181 acgtagggaa ggtttgcctc aagtggttca gacggcctgg caaatcggcc cgagacgttg
    21241 cagttcagac cgacgacggt cgcccctgca tcgctgagga gatcgggggt gatgacgctc
    21301 cccgcaccgt tgccgcagtc cagaacgacc gtgaccggcc gggagaggct gatctcgccg
    21361 aggatcgcat cccggtgcat ggcaagcgca tcgatgggag aggtgtgccc ctgttcctcc
    21421 caccccaccc ggtgcgtccc ttcgagcgcc tcttcaatcg ccgcctgctg ccggcgggtg
    21481 aacgacgacc cgtcagggtt gagcagtttg accccgttgt agggctcggg gttatgcgag
    21541 gcggtgatca tgcatcctgc gtccgcgcgc ctggtcgcat aggcgaccgt cggtgtgggc
    21601 gcaatgccgc aggtgtagac ggctgcaccg gccgagagca tgccggcgac gacgcaatgt
    21661 tccagcagtt cgcttgtcgt gcgggtatcc ctgccgacga cgaggggcga tgcgccgtcg
    21721 gcggcagcgg ctccgatgcg gagcgcgagg tcgacgaggc cgggaccgaa ctcctgacgg
    21781 attccggaag agccgaatag catacccctg tataatccct tctttcctaa aaaaaggtat
    21841 tcatcgaccg tagggacacc agtgctcgag attgaggagg ccactgaccc ctgcggtcga
    21901 cggcccgaga aagattatcc gtttattccc ccggcagccg tcgatgcagg cgattccggc
    21961 gtccgtcgtc ataccttcgc cggcaaccag gaggatatcg gcctcttccg gagaatcgac
    22021 gatccggtcc ttgaaggccc gctcgatcac cggcatcggg ccgacagggt agatgcgcat
    22081 tctgccgatc tcgcgggaga ggtcggcaag gcagggagcg tagcagtcgg gggtgcaggg
    22141 gcggagtttt cgcgcgagac agaggaacgc cgagacggcg ttgatgatcg cgcttgcagc
    22201 cccgcgctgc ttctggtccg tgagcggcgc gccgaacatg aaggagatcc gggttctggc
    22261 ccggatcggt tcgctcgtga cgaactggcc ggttcgtccg ccaaagtgcc cctccatgca
    22321 gacgcccttc ggatactggc agacatccgc gttgtagttc acagagaggt cgacgatacc
    22381 gttctcgcac ccgctgtctg cgagggtatg ctcaagtctc ctgattgcat cgttgagaaa
    22441 actcatgcta ctccttcccg ttacagagga tcgcccgcac cggcgaaccg tcggcgccgg
    22501 caagacgcat cggaagcgcc accatgtgat actctccttc cggcacggcg gcaaggtcga
    22561 gcagttcgag gacgaccgtc ccggctccga gcaaccggcg gtggaccgaa ccgtcgccac
    22621 aaaacgcctc gatcgatggc gcgtcggtcc cgatacaggc gatcccggcc tcgacgacga
    22681 ggtccgccgc ttcaggggag agcgccgggt acccggggtc gaactcctcc cgcccggaga
    22741 accatgtctt caggaggagc gtccgggcgc ccgcgaggcg gtcggcgagg ttctccgggc
    22801 cgatgagcgt ctccgcatcg ctgcagtcga ttacccgtgc cgaacccgca aggacaccga
    22861 gcgcgatccg gtcgaccgtc aggcccccct cgatgtagtg cgacggcgca tcgatatgtg
    22921 tccccgaatg cgtcccgatg gtcatctccg tcacgcggta ttgcccgtta tcgatctcgc
    22981 gaaatcgggg ccggatatca cccgggtaga ggatggcatc cgccgagagg tcgcgggtga
    23041 tgtcgtagat gatcaccgga acccttctcc tcgccatccg aactgcccga tcagcggcac
    23101 gaagcagacg ccgccgagcg ggatcgtctc caccttcccc tccctcttca cgagtttgac
    23161 gaggtcctgg cagtctcgcg ggccgatagg agcgatcaac cgtcctccct cggcaagctg
    23221 ctcgatcagg ggttcgggga cgccgggcga cgccgccgtg acgatgatgg cgtcgtacgg
    23281 cgcttcggga gggtaaccct ccgtgccgtc gccgacgacg atccggacgc cggtgacccc
    23341 tgcccgggca aggttctcgc gtgcctgttc ggcgacgccc tccaggcgtt cgatcgatat
    23401 gaccgtcccg gcgagtccgg cgaggagcgc cgcctggtag ccgctcccgc tcccgatctc
    23461 gagcacccgg tcctgcggtt tgatctcaag ctgctcggtc atgacggcga cgatgtaggg
    23521 ctgggagatg gtctgcccct ccccgatggg gagcggccgg tcctcatagg cggcgcgctc
    23581 atagcccttc ggcacgaaga gatggcgcgg aacctctcgc atcgcggcga gcacccgttc
    23641 gtccctgatc ccgcggtccc ggatctggtg ctccaccatc gcttcacgct ctctctcgta
    23701 ggggtcggtc tttgtcatct cctcctctct tttccgtcag aatcctgatt ctgccttgtc
    23761 tatcgcggca tcgatctgtt tctgcagaac ttcgggcagg ccgctgatct tgacatccag
    23821 gaacccgcgt ataatggttg cggtggcctc ctcctccgag agaccgcgcg ccatgaggta
    23881 ctcgatctcg tggcgggcga tcttgccgac cgcggcctcg tgggagagtt cggtgccggt
    23941 cagcgtcccc tcgatctcgg ggatggcgtg gatgatgccg tctttcagga tcagcccctt
    24001 gcactcgatg tggccccgtg tgccgtcctt ttcgccgagg atgtgcccgc gggagatgac
    24061 cttgccgccg gtggtgatcg cacgggtgac gagctcggcg ctcgtctgtt cggcgtcgag
    24121 gatggcccgg gagccgaggt cgaggatcga gcccggggtc gcgacgacga tgctcgagaa
    24181 ccgggccacg gcgttctttc cgaccaggcg cgccgtcggg tacatgtcga cccgattgac
    24241 cggctgcatg cagacgtaat ttgagaggaa gacgccgcct tcctcgatga tggtggcgct
    24301 ccgcgggaag acgctgatcc ccgggttcca gttgtggatc atcgtggacg tgactttcgc
    24361 gcccttgtcg acgtagatct cggtcacgcc gatatgcgca ccgtgctctt tttgccagga
    24421 actcgcgcag ccggagatga tgtgcatctc cgaaccctcc cttgcgatga atatgttatg
    24481 gacgtgctgc accggttcgt cgcggaggaa gagacaggcc tggaggggca tcgcgacctt
    24541 tgcaccctca tgagcgatga cgacgagacc ctgcgggttc tcctgttttg ccacgaactg
    24601 agtgtattta tccttgtcct tcgagacggc gttccagtag tagtctttca cccagtcgta
    24661 cttctcgagg gcggcccgga tggggagcat ctcgatcccc tcggccccgc aggtggtctg
    24721 gatcaccttc tggtcgatct ggaagaagct cccgcaccgg ttctgcatac cgacctcaag
    24781 gccggtcagg gcgaggcgtt cccggtcctc ctgcgagagg cgctccatat cggttatatc
    24841 tgtttgcaac gcaggcactc tccgtatccc ttctctttga ccacgcgcag gatctcgcgg
    24901 gggtttccgt ggcaccgtat ccggccgctg atcatcacgt ggccctggtc ggcctcgagg
    24961 tagtcgagga tgtagccggt gtgggtgatg atcaggccgc tccggcgccg gttgacgata
    25021 tggacgtcct tctcgagcag gcctgcgatc tccttgccca tcaggttcat gttctcgagg
    25081 tcgacgccgc tctccggttc gtccagcatg aggaagtcgg gctgctggac ct
//
LOCUS       DAOSEE010000014        24553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_909_length_24553_cov_241.751367, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000014 DAOSEE010000000
VERSION     DAOSEE010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 24553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24553
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_909_length_24553_cov_241.751367"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..853
                     /locus_tag="PLI76_02060"
     CDS             188..853
                     /locus_tag="PLI76_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60846.1"
                     /translation="MIPLFAPVALIVACVVACGCAAADDQPIAVNTTVPIEHGWNQAT
                     VAILEQYVLTRANQETYDMFNGSTVSPDPAILLDSNKSPLYYRYYVQKNGKTVYAVQV
                     SANKLLGTTVTAIGDFGYIGRDGSMTANESDPYGAEAGFVRGTPVPSYTDEYTRLMLD
                     SWEVGDTYARNVVRDAENAGIDLSTPLSDEEREVVGEILREQIGQREQRIQEVEKNHR
                     VSL"
     gene            complement(903..2294)
                     /locus_tag="PLI76_02065"
     CDS             complement(903..2294)
                     /locus_tag="PLI76_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-ATPase domain-containing protein"
                     /protein_id="HOI60847.1"
                     /translation="MSGTEGSWQGQVHRIIAGLEDGRARIDRGAVRNLVNTYAVRSLD
                     GTALRFSLPVFLPAPAGASGRGADGAAGILIEAIKAAVPVERDLGPIAGAGTEHPRHS
                     LPNVEPVTLIASGSAIGTDLWWPNAGNRIDGDGLRLVVRGALPYPGPPTPGAVKGVAE
                     CFAALLDALDRVVRRVPGRDLAAARDLSVDQKELRRALPGMGLVAFVADGTRPARRFT
                     DLRCHHRIAGPKEGVHIPFSCPRELDPVEVELAGSGRTVTGLGVRRREVFSVAGSNAE
                     GKSTFLHALIAGEDDHAAGDGREGLVSVNGIARAEAGERYLAGADVSLFFSRLPPGLS
                     GNPEAATGQGSGSLVMAHEIRTALFAAVPLLILDEDRSATNLLVPGCLQGDGVMPLAT
                     LLATRREALGETALLFAASSLDILIAQADRILVLSGHEAHAIDPREFRRRLDRHLAEM
                     REHLARREGGGPP"
     gene            2391..3176
                     /locus_tag="PLI76_02070"
     CDS             2391..3176
                     /locus_tag="PLI76_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOI60848.1"
                     /translation="MELTVLASGSKGNCAYIRGEHGALLIDAGLSARETLRRIANAGA
                     DASLIEAILVTHEHIDHVRGVDVLARKLHVPVYATGGTLEAFSEKYGPTSADVRRCRR
                     GEAFAVGDFSVEPFAASHDARDPCGFCVAEGDLRIGCCTDTGTITPAIFDRLASCDAV
                     LLESNHCSKMLEDGPYPAYLKSRIRSKRGHLSNSDAARCLQGLAGRIQMAVLAHVSEI
                     NNTPEKVLSSALEGLGFYSDQIAVTVAPQDPGPVHPETCGFRL"
     gene            complement(3187..4479)
                     /locus_tag="PLI76_02075"
     CDS             complement(3187..4479)
                     /locus_tag="PLI76_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOI60849.1"
                     /translation="MTGNDTGSGTGRGGPDADGAREACQTLHPGESVGLSIFRELPDG
                     LVLIDGEGRCRYLNPAFTRITGYFPGDFPTLEAWFERAHPNPAYRRNVQDTMQRLLRG
                     DQSILVASVVCRSGKVRDIEFRHAPLPAGCALLVIRDVTEQVLVEEHLRQATSELTAV
                     IEAFPDLFLRLNADGTILDSRAGRLAEVPLVSRALLGRRLQELLPAEVGVILGKAMQE
                     AARTNVPAAPMEFSCAGAGKVRHYETRIMPLRELHLLAVIREITDRKEAEEELHRHRE
                     HLEDLIAERTVELERANQRLEHLLYCIEVTERRAATDWLDSSIGQGAPGTAEPDAGRI
                     TTDADGIIVIVDGTAERLTGYAGSKLAGRTVWDVLVGDAREALTVEVLGEARSVECAQ
                     GAAIVRTDGSQFPVHLLGDAIVDAGGAVIGMVCTFWKV"
     gene            complement(4598..5830)
                     /locus_tag="PLI76_02080"
     CDS             complement(4598..5830)
                     /locus_tag="PLI76_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60850.1"
                     /translation="MEDVWQPEALRNRRLEQLQATMNDYVEKNTVQIEGNLPVFFGHV
                     AATLDRTFPDLDDRSYDDFIDGITFTLLNVTREAPTVEFLEKTLRHAMGNKRRRRGRA
                     TLDVVVALKLIDTGNYVMAIDYLMRYRNYDGRVNAAIAYCHYVLSQGTAREQGLRPND
                     RELHAREEMLNLARSCAPLDRLGVFDRKDARLNRIFWFMLDLAFTWFPGEPEFYHIGI
                     VRAKHEGDIERRRHLLDLATERFSDNKYFLVEAFNTSIEQRNGSGAAAIVKQMMQQYP
                     EDHEPIYFGLKLAILGSQPGSYANFRKLAILKQFPQRLLLLLDAALEVMCNRKAEAQL
                     CFPEVKKASSGQGHYVMALEYILRDFVEGDEDRTKRARSAFFASVDQYCMLALKIGEA
                     RAAFGLQSDNPGIPSEKT"
     gene            5960..6370
                     /locus_tag="PLI76_02085"
     CDS             5960..6370
                     /locus_tag="PLI76_02085"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOI60851.1"
                     /translation="MGYIEELKRVGRDANPFFTLMGIELGEYGDGRASLTMEVRPDML
                     NGAGWLQGGIYVALADEAIALALCTLLADDEKIATIDEHTSFIKGVNAGTIAATGRVI
                     RKGRRVAFADGEVRDAADGALLSRTSTSFAVIGR"
     gene            6507..8096
                     /gene="pyrG"
                     /locus_tag="PLI76_02090"
     CDS             6507..8096
                     /gene="pyrG"
                     /locus_tag="PLI76_02090"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HOI60852.1"
                     /translation="MKYIVVTGGVMSGLGKGITTASIGRLLKNRGYEVTAVKIDPYLN
                     IDAGTMNPAQHGEVFVLSDGGEVDLDLGNYERFLDINLKSLHNITTGKVYRNVIERER
                     HGDFLGATVQIIPHITDEIRHCIDRAAAEGINGGRVAEICLVEVGGTVGDIESMPFLE
                     AVRQMHGELAPQDMLLVHVTLVPVDTMGDHKTKPTQHSVKALRELGLQPDIIVGRSSE
                     VISPQTKKKISAFTDVPVKAVVSAKDVPDIYQIPVELEKEGLADVVCSYLALENREPD
                     PAWYRLVSQEYTNRATVAIVSKYGIEDVYISIKESLKHAGRALSTEVNIRWLDAETLD
                     LRDLADVDGILIPGGFGQRGIEGKLRAIRYARENKKPFLGLCLGFQLAVIEYARHVLG
                     IPDATSEEMGPGTHVIALLPEQEEIFNLGGTMRLGNCTVTLREDTMAARLYGKTEIVE
                     RHRHRYEVNPAFIGDLEDAGLVFSGACRDRMEVCELPDHPFYLATQFHPEFRSSPTNP
                     SPPYIGFVEACKKSRSATGIR"
     gene            8104..9021
                     /gene="guaA"
                     /locus_tag="PLI76_02095"
     CDS             8104..9021
                     /gene="guaA"
                     /locus_tag="PLI76_02095"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOI60853.1"
                     /translation="MVDIEKFIDQAVRQIRDAAGEDKVVMALSGGVDSSVCAALAARA
                     IGDRLVPIYVDTGLMRKGETERIRSLFADTNLRTVDAADEFFAALAGITDPEEKRKAI
                     GERFIRIFEREAKRTGATLLLQGTIYPDRIESEGGIKSHHNVGGMPLNIAFKGVIEPL
                     ADLYKDDVREVAGGLSLPAEIQHRMPFPGPGLAVRVLGEVTREKIAVVREANAIVEEC
                     LVEEFHPWQCFAALLGLGTGMKGDARLHGWIVAVRAVQSRDGMTADPLPLPYEALSRM
                     VARITGEIPGVARVVYDVTPKPPATIEYE"
     gene            9026..10150
                     /locus_tag="PLI76_02100"
     CDS             9026..10150
                     /locus_tag="PLI76_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOI60854.1"
                     /translation="MAEDSRVLDARYYMPAFSRTMKIVRGAGSRVRDDRGREYIDCVA
                     GIAVCSTGHCHPLVVKAICDQAHELIHCSNLYYVPNQAELAEKLVEISGLSKAFFSNS
                     GAEANDGAIKLARVRTGRKKFVAFTHGFHGRTCGSLAVTHKPAIREPFEPLSVPCTFV
                     DYGDIDALAGAVDKETAGVFVEPIQGEAGVLIPPDEFLRGVRDICDDTGALMIDDEVQ
                     TGMGRTGKWFAFQHAGITPDIVTTAKGLASGFPIGALVAREGIEFKKGEHGSTFAGGP
                     LACAAALATIGVIEEVLPDVARKGERFMKGLARHHPRGRGLIIGMSVGDRCPDVQKTC
                     AENGVLVNCAADGNLRLIPPLVITDEEIDAAVGVIDAALG"
     gene            10134..11192
                     /gene="hisC"
                     /locus_tag="PLI76_02105"
     CDS             10134..11192
                     /gene="hisC"
                     /locus_tag="PLI76_02105"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOI60855.1"
                     /translation="MRRSVRACYAGAAGYSYAKSADDVAREHGFDRVARLASNENPRP
                     PSPAALEAAAAALREGNRYPDTRASALVEALRRYHGDYRFVTGAGMDGVIETVIRTVV
                     EPGEKVAVSTPTFSFYALAAGAHGAEVVNIPRREDFSVDPAAFAGASRGAKLAFLCSP
                     NNPTGNSVPPEDVEEILDRMDGLLFLDNAYVDFADTDYRPLMRRHDNLIIGRTMSKLF
                     ALAGLRVGYAFVPEWFEPFYHRAATPFALNSVSAAAAAGALADPGPVSEARDHVRRWR
                     ERFAGEVPFPVYPSDANFVMIDVAPLTGDEAAERLAAKGVLVRSCTSFPGLGNHYIRV
                     STGEDWENIRFIEAIRDI"
     gene            11189..11701
                     /locus_tag="PLI76_02110"
     CDS             11189..11701
                     /locus_tag="PLI76_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase family protein"
                     /protein_id="HOI60856.1"
                     /translation="MMTGITGTPGTGKTAVAAELERRGHRIVHLTDTVRPYVVEEDRA
                     RQTLVVDVDRWVEEFEPVEGIVEGHLAHLLPCDRVVVLRCRPDVLAARLAPRGYPAEK
                     IAENRDAEALDVILIETLEEHPDEHILEVDTTALSVGECADRIEEFIRGELPSSYGSI
                     DWTDHLGFSA"
     gene            11698..12300
                     /locus_tag="PLI76_02115"
     CDS             11698..12300
                     /locus_tag="PLI76_02115"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOI60857.1"
                     /translation="MTLDQFRPHVQGIVQPVVDLARSIGLTPNGLTLASFFVSILAGL
                     AFYAGGIVLGVVMVALNAVFDALDGALARDMGIASPRGDFLDHVIDRYADIFIITGIF
                     AGGAAPWPIGVFALTGVLMSSYLGTQAQAVGVGRFYGGILGRADRLVLIIIAGVLTVL
                     IPGEIGGLNYLGWLLLIFGVFGHYTAFQRFAHVWRQMEKD"
     gene            12440..13324
                     /locus_tag="PLI76_02120"
     CDS             12440..13324
                     /locus_tag="PLI76_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOI60858.1"
                     /translation="MQVSNPVLIGGVPLDAFFAFILALIGFLFLGNLAYMLLRQALDG
                     RVSPGTAKWASAILQYAVMFGGVYVSARHLLDFDPRAFAASLGILGIIVAVSSTQITQ
                     NILAGILITINRPVQLEEWIVVGELPTTGLCKVRDISFTTTILQGLDGGLILMPNSSI
                     IASKVVNYSRNRLIEIRVSLSVPVSVDIARVRDIALAVAHDDSRVIRTPGAAGRSAVH
                     DLFDLPYNRRLRSDRPDLIGLGPAVHTVSVSDGWIKMVVRIWTQKIPQKDDITSRYLR
                     EILGRLEAEDIPVKAEAA"
     gene            13364..14287
                     /locus_tag="PLI76_02125"
     CDS             13364..14287
                     /locus_tag="PLI76_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOI60859.1"
                     /translation="MQESVPVLIGGVPLDAFLAFIFAFIASLFLGNLAYMLLRRLLDG
                     RASRGKVKWAAAILQYGITVGGVYASARYFLDFELTAFFASLGILGIAVAFSSRQIIQ
                     NAVAGVLITINRPIQLEEWVVVSGRPETGLCRVQDISFTTTILQALDGGLVLTPNSSI
                     ITSKVINYSRAGLLEVSVPLAVPVTTEIARVQEIAVAAARDHPLILPHVEPAERSAVQ
                     NLFDLPYIGRLSSDLPRPDLRLFEPKVQVASIKEEWVRLTVRIWIRQIPGKDRIVSEY
                     LDAVIGHLSAEGLLEAKKPEDDPAPGFPGAA"
     gene            14346..15491
                     /locus_tag="PLI76_02130"
     CDS             14346..15491
                     /locus_tag="PLI76_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate/molybdate transporter"
                     /protein_id="HOI60860.1"
                     /translation="MMEKTRSTGNGIRFTLEEIAGAVGDFGTIFPILLGVAIVSPDVN
                     VSHFFFFLAAWFIIAGLYYRLPIPIEPMKAIGAVVIAGGLSSGEIVASGLIVGALFLL
                     LGLVGGMTWLGDRIPKSVIRGVQAGLALILLRTSLGYIVDDVLFAIVSIAIIVVFFIA
                     SARTRIPDISALLVLLIGLAVGIATQGMPPFRLIPLPPLIVPSLPDFVAGIWDLALPQ
                     IPLTIANAILATSLLTYDLFPKKGVDPDRLSRTIGAMNLISTPLGGFPMCHGAGGLAA
                     MYRFGARTGGANIVAGLFILVFAVVFAPPEVLTLIPFGVFGALLVFVAIELGKHGIKT
                     ESYLVTGVIAVLALALGLTIAFIAGMALAYALEWWKKEQGAGQKPGA"
     gene            15549..15803
                     /locus_tag="PLI76_02135"
     CDS             15549..15803
                     /locus_tag="PLI76_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HOI60861.1"
                     /translation="MDAITALGLIAGSLTTLSFAPQVVRAWRTRSTADLSLATLIVFL
                     AGLLLWLAYGVVRGDPAIVAANAVTAVFVGLILSIKTKHG"
     gene            complement(15784..16851)
                     /locus_tag="PLI76_02140"
     CDS             complement(15784..16851)
                     /locus_tag="PLI76_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOI60862.1"
                     /translation="MIEFDRVSLALGSFRLKEISLTIGRGDYYFIVGPSGAGKTVLLE
                     TIAGLHRPESGRVLLRDEEITTLPPEKRRVALVYQDYSLFPNMRVIDNVSYGLRVQGM
                     RKKEARDEVVPLLERFGIAHLADRYPGTLSGGEQQRVSLARAIAVKPDILLLDEPLSA
                     LDPITQERFIGDLRRLHREDGLTIVQVSHSRREAHLLATRMAVIIDGYLVDEGKAGVV
                     LNTPRSRDVATFVGIENILDGTVTANEDGHATVDAGGRTFEAMTDAAPGEEVSLCIRA
                     DDIILAVDGDRHSSARNTMTGTVVSVVENGPVAEVKVDAGVVLTAVLTRRSVRDLLLV
                     AGTAVTLSIKATAIHVIRASS"
     gene            complement(16838..17728)
                     /locus_tag="PLI76_02145"
     CDS             complement(16838..17728)
                     /locus_tag="PLI76_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI60863.1"
                     /translation="MDMKTQTRSTDPLPADGEPVAATRWKRERKRYTDWCTLGFCILG
                     GLLVGITVLALANITMAELADPAHLIKIATSSTVIESILLTFGAGASAVLLLIVFGTP
                     LAYILARSRPSRFKGVVESIIDIPLILPHTVAGLLVYLLFMRRGWLGAPLSDIGLAFE
                     DALPGTIVAMLFVASPFYINSMREGFEKVPVHLENVARTLGAGTFTMFRTVSLPLSLR
                     HMYSGAILAWGRAIGEFAGVIMIAYYPFIISTLIYYSFTTDGIRASRSIAFTVILISF
                     GVFYLLRRMTRHLGRYDDRV"
     gene            complement(17795..18886)
                     /gene="wtpA"
                     /locus_tag="PLI76_02150"
     CDS             complement(17795..18886)
                     /gene="wtpA"
                     /locus_tag="PLI76_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tungstate ABC transporter substrate-binding
                     protein WtpA"
                     /protein_id="HOI60864.1"
                     /translation="MKSIRMVAFIALIVAAAVLICGCTGTTNTDSTPGTGAQTTTPAS
                     TAAPTENIQVKIFHAGSLTGPFEKVKAEFEAKYPGTTVLLEPAGSIDCVKKITENGKP
                     ADVLASADYYLIPEMMIPDHADWYLTFAKNLMVLTYTNESKYASEITADNWYEILGRD
                     GVRWGFSDPNSDPCGYRTPMVIQLAESHYKNDQIFENLVSKHSNITVTEENGISTIHA
                     GDPKPDGAVLTIRPKSVELVQMVQAGGLDYAWEYRSVAVQNDLRYIELPEAIDLSSID
                     FEDTYATVQIETKKGDSVAIFTGSPIIFGITVPKIAEHPDLGVAFIEMLIDTTGQEIL
                     VADGQPPIVPADGYGNVPDVLKPLVAMRS"
     gene            complement(19009..20094)
                     /locus_tag="PLI76_02155"
     CDS             complement(19009..20094)
                     /locus_tag="PLI76_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI60865.1"
                     /translation="MVIIEARDIRKVYGNLEVLHDVDLSVEEGEILGLIGPSGSGKST
                     LLRILDLIEPPSGGELSVFGTDTVAERSRWLDLRRRMGMLFQRPIVFNASVYDNVAMG
                     LRYRGVSGSDIDRKVKEALDAVGLSRYIKSRATDLSGGEQQRVALSRVLVTDPEILFL
                     DEPTANLDPGSTATIERIVADLNREAGMTVLISTHDLAQGQRLAHRIAVMIEGTVAQA
                     GSPHAVFHEPGSRKIAGFVGVQNIIPGRIVSRQGGLTTVAAKDRRIISATPSPADEVF
                     VVIRGEDISLHRREPGYEEAENLFPARVTAIESMAPFVNVTVDCGCDLVVLMPARRAE
                     ALEIHVGMEVWVSLPAKVVHLVPQNGP"
     gene            complement(20099..20794)
                     /locus_tag="PLI76_02160"
     CDS             complement(20099..20794)
                     /locus_tag="PLI76_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI60866.1"
                     /translation="MVNGNPIVEGFIEAIRLIVSLDPQVVEITIRSLYVSLTATFFAA
                     LIALPLGALIYFYEFRGKHAVVSTLQTLYALPTVIVGLVMFLLLSNIGPFGFLRLLYT
                     PGGMIVTQTVLIIPLLMGLTVSALSGIDRDKRYTITALGASRLQTIVTIIVESRFAVM
                     SSVLLGFGRAISEVGTVMIIGGNIRGATRVLTTAIALNTSMANYSVSIALGIILLAVA
                     LGVNIALSLVQRR"
     gene            complement(20850..21824)
                     /locus_tag="PLI76_02165"
     CDS             complement(20850..21824)
                     /locus_tag="PLI76_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOI60867.1"
                     /translation="MKMNVAILSAGTIAILFILALLAGCTGTAPGENVTPNVTTTAPA
                     AGANVLKIATTTSLENTGLLAELERVYERETGMDLQFIAQGTGQALDTARRGDVDLVL
                     VHAPALEKEFEDEGYGIDPRCIAYNYFIIVGPEDDPAKIRNMSAVEAFRTIAIAGMNN
                     TPGVAFVSRGDNSGTHAQEKALWKEAGYNYTTEILDSGAWYVDAGKGMGDTLILADEK
                     KAYTLSDEGTYLSFANRLALVPIISESTELMNRYSAIAVNPAKYPNTNARGAADFINW
                     LISDEGKQLIGDFGKVEFGKPLFVPLYAPECTGPPFNCTCAEPVPTPA"
     gene            complement(22040..22975)
                     /locus_tag="PLI76_02170"
     CDS             complement(22040..22975)
                     /locus_tag="PLI76_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOI60868.1"
                     /translation="MRSRTWMPILGIMVVLLAACAVSVTAQQAANPGVLRIATTTSLY
                     DTGLLDNLEDMYENVSGVDVQITAQGTGQALDTARRGDVDLVLVHSPGLEQEFIDQGY
                     GINPHCFAYNNFLIVGPDSDPAKIKNMTPEEAFKTIAIAGMNDTPGVAFVSRGDNSGT
                     HNREQQIWKAAGYNYTTDIQDSGDWYVETGSGMGETLTIANQKEAYTLSDIGTYLAFK
                     SQLNLVQLVTEGGELMNRYSAIAVNPEKNPNVNIDEANRFIDWITSNETKEFIGNFGV
                     AEFGQPLFVPLYPPECSQPPFNCTTCSSGEVNATA"
     gene            23139..24389
                     /gene="hisD"
                     /locus_tag="PLI76_02175"
     CDS             23139..24389
                     /gene="hisD"
                     /locus_tag="PLI76_02175"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOI60869.1"
                     /translation="MWQALEIETWIAGRRSEIDRVRGPVSEIIARVRSEGDAALIDLT
                     KRFDGIDLDEIAVSDEEREAAYDRVDAQLIESLAEAEARISRFHELQRGRDLWLQEME
                     PGITLGVRTTPLSRIGAYVPGGRAAYPSTALMCTVPAKVAGVREICCCTPPPTNPITL
                     VALDIAGVDEIYRVGGAQAIAAMALGTETIPRVEKIVGPGNVYVTAAKMLLRDEVEID
                     FPAGPSEIAILADGTANPTFVAADILAQAEHDPNAACVLVTTDAVLADRVGAEVKRMA
                     AGAVRREIIDRALSHSGYIVAADIDAAVETVDAVAPEHLSIQVADPLAVLTRVRNAGS
                     IFVGPYAAVACGDYASGTNHVLPTAGYARLYSGLDVNHFCRRATVQVITREGLEAIGD
                     VVETIADAEGLHAHAESVRVRRRG"
BASE COUNT         4291 a         7979 c         7830 g         4453 t
ORIGIN      
        1 tgtaggcttc agattgagaa tcatcgaacc ggatcccgat caactcgcac atgctgtggt
       61 agggcacctg cacgaagttc tggttccccc gccggccgcc tgaccgcggg agaacgccat
      121 gacgaagggc aaccgttatc agcgcaaaga tagacccctc aaagaggtcg tatccatgaa
      181 ccgcagcatg atccccctgt ttgcaccggt agcactcatc gtggcctgtg tcgttgcatg
      241 cggctgtgca gcagctgatg atcaacctat agccgtcaat acaaccgtcc ctatagaaca
      301 cggatggaac caggcgacag tggcaattct tgaacaatac gtgcttacca gggccaacca
      361 ggaaacatac gatatgttca acggttcgac ggtatctccg gatcctgcaa tcctgctcga
      421 ttccaacaaa agccccctgt attaccggta ttacgttcag aagaacggca agaccgttta
      481 cgcggtccag gtgagcgcga acaaactgct cggcacaacg gtgactgcga tcggcgattt
      541 cggttacata gggcgagacg gatcgatgac cgcgaacgaa agcgatccgt atggcgcaga
      601 agccgggttt gtccgcggta ctccggtccc ctcgtatacc gatgagtata ctcgattgat
      661 gctcgattcc tgggaagtcg gggacaccta tgcccggaat gttgtgcgag acgcagaaaa
      721 tgccggaatc gatctctcaa cgccgctctc cgatgaggaa cgggaggtcg tcggagagat
      781 ccttcgagaa cagatcgggc aacgagaaca gagaatacaa gaggtcgaaa agaaccatcg
      841 ggtgagcctc taacagccgg gttgcacacc catctcccaa acttcgcgtt ctctgcgcga
      901 gttcacggcg gaccaccccc ctccctcctc gccaggtgct cccgcatctc cgcgaggtgc
      961 cggtccagac gccgccggaa ctcacgggga tcgattgcgt gcgcttcatg gcccgacaac
     1021 accaggatcc ggtcggcctg tgcgatcagg atatcgagcg acgaggccgc aaagaggagc
     1081 gccgtctcgc cgagcgcctc gcgccgggtc gcaagcaggg tcgcaagcgg catcaccccg
     1141 tcgccctgga ggcagcccgg aaccagcagg ttcgtcgcgg aacggtcctc gtcgaggatg
     1201 aggagcggca cggcggcaaa gagcgcggtc cggatctcgt gcgccatgac gagcgacccg
     1261 ctcccctggc cggttgccgc ctccggattg cccgaaagcc cgggcgggag gcgggagaag
     1321 aagaggctga cgtcggcccc cgcaaggtag cgctcgcccg cctccgcccg ggcgatcccg
     1381 ttcacactga ccaatccctc ccgcccgtcc ccggcggcgt ggtcgtcctc tcccgcgatg
     1441 agcgcgtgga ggaaggtgct cttcccctcg gcgttcgagc cggcgaccga aaagacctcc
     1501 cgccgccgga ccccaagccc cgtcacggtc cggccgctcc cggcaagttc cacctcgacc
     1561 gggtcgagct cccgcgggca cgaaaagggg atgtgcacgc cctccttcgg ccccgctatc
     1621 cggtggtggc accggaggtc ggtgaacctg cgggccgggc gcgtgccgtc tgcgacgaac
     1681 gcgacgagcc ccatccccgg gagagcgcgc cggagttctt tctggtcgac ggagagatcc
     1741 cgcgccgcgg caaggtcacg gcccggcacc cggcgcacga cgcggtcgag agcatcgagg
     1801 agcgctgcaa agcattctgc gacaccctta accgcgcccg gtgtcggcgg gccggggtaa
     1861 gggagagcgc ccctgacgac cagccggagg ccgtcgccat cgatacggtt cccggcattc
     1921 ggccaccaga ggtcggtccc gatggcgctc cctgacgcga tcagcgtgac cggttcaacg
     1981 ttcgggagag agtggcgggg gtgctccgtc ccggcgccgg cgatcgggcc gaggtcccgc
     2041 tcgacgggaa ccgccgcctt gatcgcctcg atgaggatcc cggcagcccc gtcggcaccc
     2101 cggccggatg ccccggccgg cgccgggagg aagaccggga ggctgaaccg gagcgccgtc
     2161 ccatcgagcg accggacggc gtaggtgttg acgaggttcc gcaccgctcc ccggtcgatg
     2221 cgtgcccgac cgtcctcaag ccccgcgatg atccggtgaa cctgcccctg ccaggagccc
     2281 tccgtcccgc tcattacctc tcgtcaggag atagtatccc gaaccacaag gctctttgta
     2341 caccctcccc gccgcgtgct taaatagccg cgcagaccac caattgtata atggaattga
     2401 ccgtccttgc gagcggaagc aagggaaact gcgcctatat ccggggggag catggtgcgc
     2461 tgctcatcga cgcggggctc tccgcacgcg agaccctccg ccgcatcgca aacgccggcg
     2521 ccgacgcatc gctcatcgag gcgatcctcg tcacccacga acacatcgat cacgtcaggg
     2581 gggtcgacgt cctcgcgcgg aagcttcacg ttccggttta cgcgaccggc ggcacgcttg
     2641 aggcgttctc ggagaaatac gggccgacgt cggcagatgt ccggcgatgc cggcgcggcg
     2701 aggcgttcgc cgtcggcgac ttctcggtcg aacccttcgc cgcttcccac gatgcccgcg
     2761 acccctgcgg gttctgtgtc gccgagggcg acctccgcat cgggtgctgc accgacaccg
     2821 gcaccatcac ccccgccata ttcgaccgcc tcgcctcctg cgacgccgtc ctcctcgaga
     2881 gcaaccactg ctcgaagatg ctcgaggacg gcccctaccc cgcgtacctc aagagtcgta
     2941 tacggtcgaa acgcgggcac ctctcgaact ccgacgccgc ccgctgcctc caggggcttg
     3001 ccggccggat ccagatggcg gtgctcgccc acgtcagcga gatcaacaac acgcccgaga
     3061 aggtgctctc ctccgcactc gaggggctcg ggttctactc cgaccagatt gccgtcaccg
     3121 tggcgcccca ggatcccggc ccggtgcatc ccgagacatg cggtttccgg ctctgaccgc
     3181 gcgccctcac accttccaga acgtgcagac catgccgatc acggcgcccc cggcgtcgac
     3241 gatcgcatcg cccaggaggt ggaccgggaa ctgtgacccg tcggtcctca cgatcgccgc
     3301 accctgcgcg cactccacgg atctcgcctc gccgagcacc tccacggtga gggcttcccg
     3361 ggcatcgccg acaagaacat cccataccgt gcggccggcc agcttgcttc cggcgtagcc
     3421 ggtcaggcgt tcggccgtcc catcgacgat gacgattata ccgtccgcat ccgtcgtgat
     3481 cctccccgcg tcgggttcgg ccgttcccgg ggcgccctgc ccgatcgatg agtccagcca
     3541 gtcggttgcg gcccggcgtt ccgtcacctc gatgcagtag aggaggtgct caagccgctg
     3601 gtttgcgcgt tcgagctcca cggtccgctc cgctatcagg tcttccaggt gctcgcggtg
     3661 gcggtgcagt tcctcttccg cctctttccg gtccgtgatc tccctgatga cggcaaggag
     3721 atgcagctcg cgcagcggca tgatccgggt ctcgtagtgc cggacttttc cggctcccgc
     3781 acaggagaac tccatgggcg ctgccggcac gtttgtccgg gcggcctcct gcatcgcctt
     3841 cccgaggatc accccgacct cggcggggag cagctcctgc agacgccggc cgagcagcgc
     3901 ccgggagacg agaggcacct ccgcaagcct cccggcccgt gagtccagga tcgtgccgtc
     3961 ggcgttcagg cggaggaaga ggtcggggaa cgcctcgatc accgccgtca gttcggatgt
     4021 ggcctgccgg aggtgttcct cgaccaggac ctgctcggtg acgtcgcgga tgacgagcag
     4081 ggcgcacccg gcagggagcg gcgcatgccg gaactcgata tcccgcacct ttccgctccg
     4141 gcagaccacg ctcgcgacaa ggatgctctg gtcgccccgg agaagcctct gcatcgtatc
     4201 ctgcacgttc cggcggtatg cgggattggg gtgggcacgc tcgaaccagg cctccagggt
     4261 cgggaaatcc cccgggaagt acccggtgat ccgggtaaac gccgggttca ggtaccggca
     4321 tctcccttca ccatctatca gaacaagacc atccggcagt tcgcggaaga tcgagaggcc
     4381 gacgctctct cccgggtgca acgtctggca ggcctcccgt gcgccgtcgg catccggtcc
     4441 tccccggccg gtgcccgaac ccgtatcgtt tcccgtcatg gtaaccaacc ctccccggca
     4501 catccgtata tcccggccgg caaacgctcc ctgcagaggg atttgcctgt tactcttgat
     4561 aaattcatcg ttgtaccttt cccttttatc gttgcgatca ggttttttct gagggtatcc
     4621 cgggattatc gctctgcagc ccaaaggcgg ctcgggcctc cccgatcttc agggcgagca
     4681 tgcagtactg gtcgacgctt gcaaagaaag cggatcgtgc ccgcttcgtc cgatcttcgt
     4741 cgccttcgac gaaatcacgg aggatgtact cgagcgccat aacgtagtgc ccctgcccag
     4801 aagaggcctt cttcacctcc ggaaagcaga gttgcgcctc ggcttttcgg ttgcacatca
     4861 cctcgagggc ggcatcgagc agcaggagca ggcgctgggg aaactgcttc aggatcgcga
     4921 gcttccggaa gttggcgtag gaccccggct gcgacccgag tatcgcgagt ttcagcccga
     4981 agtagatcgg ttcatggtct tccgggtact gctgcatcat ctgcttgacg atggcggccg
     5041 caccgctccc gttgcgctgc tcgatgctgg tgttgaacgc ctcgaccagg aaatacttgt
     5101 tgtccgagaa ccgttccgtt gcgagatcga ggagatgccg gcgacgctcg atatccccct
     5161 cgtgctttgc ccgcacgatc ccgatgtggt agaactcggg ttcgccaggg aaccaggtga
     5221 acgccaggtc cagcatgaac cagaagatcc ggttcaggcg ggcgtctttg cggtcgaaga
     5281 ccccgagccg gtcgagcggc gcacacgacc gcgcaaggtt cagcatctcc tcgcgggcgt
     5341 ggagttcccg gtcgttgggg cgaaggccct gttctctggc ggtcccctgc gagaggacgt
     5401 agtgacagta ggcgatcgcg gcgttgaccc ggccgtcgta gttacgataa cgcatcaggt
     5461 aatcgatcgc catgacgtaa ttgccggtat cgatcagttt gagcgccacc accacgtcga
     5521 gggttgcccg ccctctcctc cgccgcttgt tccccatagc gtgccgcagg gtcttctcga
     5581 ggaactccac cgtcggggcc tcgcgcgtca cattgaggag cgtgaaggtg atcccgtcga
     5641 tgaagtcgtc atacgaccgg tcatccagat cgggaaacgt ccggtcgagg gtggcggcaa
     5701 cgtgcccgaa gaagaccggc aggtttcctt ctatctggac ggtgttcttc tcgacgtagt
     5761 cgttcatggt agcctggagc tgctcaagtc tcctgttccg gagcgcctcg ggctgccaca
     5821 cgtcttccat accctacagg tggagatccg ggaagatatt tgttcctata ttttttctgg
     5881 cgctccaggg cctccgggcg aattgccggc aagccttatc ccggccggcc tcgaaccttt
     5941 ccgcgaggat ggtggtcgcg tgggctacat agaagaactg aagcgggtgg gaagggatgc
     6001 aaatccattc tttacgctga tggggataga attgggcgaa tacggcgacg ggcgcgcgag
     6061 tctcacgatg gaggtccggc cggatatgct gaacggcgcc gggtggctcc agggcgggat
     6121 ctacgtggcg cttgcagacg aggcgattgc acttgcgctc tgcacgcttc ttgccgatga
     6181 cgagaagatc gccacgatcg acgagcacac cagtttcata aaaggcgtca acgcggggac
     6241 gatcgccgcc acggggagag tcatccgcaa aggccgccgg gttgcgtttg ccgacggcga
     6301 ggtcagggat gcggcggacg gcgcgctcct ctcccggacg tcgacatcgt ttgcggtcat
     6361 cgggagatag cgccgcggtg tgcagagaga tcggccgtcg caccggcagg gattctcccg
     6421 ggggagtgcc ggaacaatac attctcaatg ccagagatcc catacatgac tgagagaccc
     6481 tggaagaacc ggaggggaac gagagtttga agtatatcgt tgtaaccggc ggcgtcatga
     6541 gcggactcgg gaaaggcatc accaccgcat ccatcggccg cctcttaaaa aatcgcggct
     6601 atgaggtgac ggcggtcaag atcgacccgt acctcaacat cgatgccggc accatgaacc
     6661 ccgcccagca cggggaggtc ttcgtcctct ccgacggcgg agaggtcgat ctcgatctcg
     6721 gcaactacga gcgattcctg gatatcaact taaaatcgct ccataacata acgaccggga
     6781 aggtataccg aaacgtcatc gagagagaac ggcacggcga cttcctcggc gcgaccgtcc
     6841 agatcatacc ccacatcacc gacgagatcc ggcactgcat cgatcgagcg gcggcggagg
     6901 ggatcaacgg cggcagggtt gcggagatct gcctcgtgga ggtcggcggc acggtcgggg
     6961 atatcgagag catgccgttt cttgaggccg tccgccagat gcacggggaa cttgcgccgc
     7021 aggatatgct cctcgtccac gtcaccctgg ttccggtgga taccatgggc gaccacaaga
     7081 ccaaacccac gcagcactcg gtcaaggcgc tccgcgaact cgggttgcag cccgacatca
     7141 tcgtagggag gagcagcgag gtgatcagcc cgcagacgaa gaagaagata tccgccttca
     7201 ccgacgtccc ggtcaaggcc gtcgtatccg caaaggatgt ccccgatatc taccagatac
     7261 cggtggagct cgagaaggag gggcttgcgg atgtcgtctg cagttacctc gcccttgaga
     7321 accgcgaacc ggaccccgcg tggtaccgcc tggtctcgca ggagtacacg aaccgggcga
     7381 cggtcgccat cgtgagcaag tacgggatcg aggatgtcta catatccatc aaggagtcgc
     7441 tcaagcacgc gggaagagcg ctctccaccg aggtgaacat acgctggctg gatgcggaga
     7501 cgctcgatct tcgcgatctc gccgacgtcg acggcatcct gataccgggc gggttcgggc
     7561 agcgcgggat cgaggggaag ctacgggcga tccggtatgc ccgcgagaac aagaaaccct
     7621 tcctcggcct ctgcctcggg ttccagctcg cggtgatcga gtacgcgcgc cacgtcctcg
     7681 gcatccccga cgccacgagc gaggagatgg ggccgggaac gcacgtcatc gctctcctcc
     7741 ccgagcagga agagatcttt aacctcggcg gcacgatgcg ccttgggaac tgcaccgtca
     7801 ccctcaggga ggacacgatg gcggcccgcc tctacgggaa gacagagatc gtggagcggc
     7861 accgccaccg ttacgaggtg aacccggcgt tcatcggcga cctcgaagat gccgggctcg
     7921 tcttctcggg cgcgtgcagg gaccggatgg aggtctgcga actcccggac catcccttct
     7981 accttgcgac ccagttccac ccggagttca ggtcgagccc gacaaacccc tccccgccct
     8041 acatcgggtt tgtggaggca tgtaagaaga gcaggtccgc taccgggatc aggtgaaaac
     8101 agtatggtag acatcgagaa gtttatcgat caggcagtcc ggcagatacg tgatgctgcc
     8161 ggcgaggata aggtcgtgat ggcgctctcc ggcggcgtgg actcgtcggt ctgtgcggcg
     8221 ctcgccgccc gcgcgatagg cgatcggctc gtcccgatat acgtcgatac cggcctcatg
     8281 cgcaaaggtg agacggagcg gatccgctcc ctctttgccg atacaaacct ccgcaccgtg
     8341 gatgccgcgg acgagttctt tgcggcgctc gcaggcatca ccgaccccga agagaagcgc
     8401 aaggccatcg gcgagcggtt catccggata ttcgagcggg aggcaaagcg gaccggagca
     8461 acgctcctcc tccaggggac gatctacccc gaccgcatcg agagcgaggg gggcatcaag
     8521 agccaccaca acgtcggcgg gatgcccctc aacatagcat tcaaaggcgt catcgagccg
     8581 cttgcggacc tctacaagga cgatgtccgc gaggtcgccg gcggtctatc gctcccggcg
     8641 gagatccagc accggatgcc cttcccgggc ccggggcttg ccgtccgggt gctcggcgag
     8701 gtgacgcggg agaagattgc ggtcgtccgg gaggcaaacg ccatcgtcga ggagtgcctg
     8761 gtggaggagt tccacccctg gcagtgtttt gccgcgcttc tcgggcttgg gaccggcatg
     8821 aagggcgatg cccggctcca cggctggatc gtcgccgtcc gggccgtcca gtcccgcgac
     8881 ggcatgaccg ccgatccgct ccctctcccc tacgaggcgt tatcaaggat ggtggcccgg
     8941 atcaccggcg agatccccgg cgtcgcccgg gtggtctacg acgtcacccc aaaacccccc
     9001 gcaacgatcg agtatgagtg atactatggc agaagattca cgcgtgcttg atgcacgcta
     9061 ctacatgccc gcgttctccc ggacgatgaa gatcgtccgt ggcgcggggt cgcgtgtccg
     9121 ggacgaccgg gggcgcgagt atatcgactg cgtggcaggc attgcggtct gcagcaccgg
     9181 ccactgccac ccgttggtgg taaaagcgat ctgcgaccag gcccatgagc tgatccactg
     9241 ctcaaacctc tactacgtcc cgaaccaggc ggaactcgcg gagaaactcg tcgagatatc
     9301 aggcctttcg aaggcgttct tctcgaactc cggcgcggag gcaaacgacg gcgcgatcaa
     9361 actcgcccgc gtccggacgg ggaggaagaa gttcgtcgcg ttcacccacg ggttccacgg
     9421 ccggacatgc gggtcgcttg cggtcaccca caagcccgcg atacgggagc cgttcgagcc
     9481 actctcggtc ccctgcacct tcgtcgacta cggcgacatc gacgccctcg cgggcgcggt
     9541 cgataaggag accgccgggg tcttcgtcga gcctatccag ggcgaagcag gcgtcctgat
     9601 accgcccgac gagttcctca ggggcgtccg ggatatatgc gacgataccg gcgctctgat
     9661 gatcgacgac gaggtgcaga ccggtatggg ccggacgggg aagtggttcg ccttccagca
     9721 tgccggcatc acccccgaca tcgtcacgac tgcaaaggga ctcgcgagcg ggtttcccat
     9781 aggcgcgctc gtcgcccgcg aggggatcga gttcaagaag ggcgagcacg ggagcacgtt
     9841 tgccggaggc ccgctcgcct gcgctgcggc gctcgcgacg atcggcgtca tcgaggaggt
     9901 tctccccgac gtggcccgga agggcgagcg gttcatgaag gggcttgccc gtcaccatcc
     9961 ccgcggccgc ggcctgatca tcggcatgtc ggtcggcgac cggtgcccgg atgtgcagaa
    10021 gacctgcgcc gagaacggcg tgctcgtcaa ctgcgccgcc gacgggaacc tccggcttat
    10081 cccgccgctc gttatcacgg atgaggagat cgacgcggct gtcggtgtca tcgatgcggc
    10141 gctcggttag ggcgtgctat gcaggcgcgg cgggctactc ctacgcgaag tcggccgacg
    10201 acgtcgcgcg ggagcacggt tttgaccggg tggcccgcct cgcgagcaac gagaaccccc
    10261 ggccgccctc gcctgccgcg ctcgaggcgg cagcggccgc tctcagagag gggaaccgct
    10321 acccggacac gcgggcatcg gccctcgtcg aggcgctccg ccgctaccac ggcgattacc
    10381 ggttcgtcac cggcgccggg atggacggcg tcatcgagac ggtgatccgg acggtcgtcg
    10441 agcccggcga gaaggtggcc gtctcgaccc ccaccttctc gttctacgcc ctcgcggccg
    10501 gggcacacgg cgcagaggtc gtgaatatcc cccgccggga agacttctcg gtcgatccgg
    10561 cggcttttgc cggggcatcg cgtggggcga aacttgcctt cctctgctcc ccgaacaacc
    10621 cgaccggaaa ctcggtcccc ccggaggatg tcgaagagat cctcgatcgc atggacgggc
    10681 tcctcttcct cgacaacgcc tacgtcgatt ttgcggatac cgactaccgc cccctcatgc
    10741 ggcgccacga taacctgatc atcgggcgga cgatgtcgaa gttattcgcc cttgccggcc
    10801 tccgggtcgg ctacgccttc gtgccggagt ggttcgagcc gttctaccac cgggcggcaa
    10861 caccgtttgc cctgaactcg gtctcggcgg cggccgccgc cggggcgctt gccgatccgg
    10921 gaccggtcag cgaggcccgc gaccacgtcc ggcggtggcg ggaacggttc gccggagagg
    10981 tgccgtttcc ggtctatcct tcggatgcaa acttcgtcat gatcgatgtt gctcccttaa
    11041 caggcgacga ggcggcggag cgcctcgcgg cgaagggcgt cctcgtgcgg tcgtgcacca
    11101 gtttccccgg gctcgggaac cattacatca gggtcagcac aggcgaagac tgggagaaca
    11161 tccggttcat cgaggcgatc cgcgacatat gatgacgggc atcaccggga cgccggggac
    11221 cgggaagacg gccgttgcgg ccgaactcga gcggcggggc caccggatcg tccacctgac
    11281 cgatacggtg cggccctacg tcgtcgagga ggaccgcgcc cggcagacgc tcgtggtgga
    11341 cgtcgaccgg tgggtggagg agttcgagcc ggtcgagggg atcgtcgagg gccacctcgc
    11401 gcacctcctc ccctgcgacc gggtggtggt gctccggtgc cgcccggacg tcctcgccgc
    11461 gcgcctcgcc ccgaggggtt acccggcgga gaagatagcg gagaacaggg acgcggaggc
    11521 gctcgacgtc atactgatcg agacgctgga ggagcacccc gatgagcata tcctcgaggt
    11581 ggatacgacc gccctatccg tcggagagtg cgccgaccgg atcgaggagt ttatccgggg
    11641 agaactgcca tcctcctacg ggtccatcga ctggaccgat catctgggtt ttagcgcatg
    11701 acacttgacc agttccggcc gcacgtgcag ggcatcgttc agcccgtggt agacctcgcc
    11761 cggagtatcg ggctcacccc gaacgggctc acccttgcct cgttcttcgt atcgatactt
    11821 gccggcctcg cgttctatgc cggggggatc gtgctcggtg tcgtcatggt tgccctgaac
    11881 gccgtctttg atgcgcttga cggggcgctt gcccgggata tggggatcgc gagtccccgg
    11941 ggggatttcc tggaccacgt catcgaccgc tacgccgaca tattcatcat caccggcata
    12001 tttgcgggcg gcgctgcacc ctggccgatc ggggtctttg ccctgaccgg tgtcctgatg
    12061 tcctcctacc tcggcaccca ggcccaggcg gtcggggtcg gcaggttcta cggcggcatc
    12121 ctcggccgcg cggaccgcct tgtgctgatc atcatcgccg gcgtgctcac ggtcctgatc
    12181 cccggggaga tcggggggtt gaactacctc gggtggctcc ttctcatctt cggcgtcttc
    12241 gggcactaca ccgcattcca gcggttcgcc cacgtctggc ggcagatgga gaaagactga
    12301 cgcggacggt gctgccggtt ccctcgcgcg agagcggcac aacaccgcgc aaaccggccc
    12361 ggaacggagg acctctcgat acccttgagc cgcccgagag aggagaagtt aactctcctg
    12421 ccggcccacc tcttcccaca tgcaggtgag caatccggtt ctcatcggcg gggtacccct
    12481 cgatgcgttc ttcgcgttca tcctcgccct catcgggttc ctcttcctcg gcaaccttgc
    12541 ttacatgctc ctccggcagg ccctcgacgg ccgggtctcc ccggggacgg ccaaatgggc
    12601 atcggccatc ctccagtacg ccgtcatgtt cggcggggtc tatgtgagcg cccgccacct
    12661 cctcgacttc gacccgaggg cctttgccgc atcgctcggt atactcggta tcatcgtcgc
    12721 cgtatcgtcg acccagatca cccagaacat ccttgccggc atcctgatca cgatcaaccg
    12781 cccggtccag ctcgaggagt ggatcgtcgt cggggagcta ccgacgacgg ggctgtgcaa
    12841 ggtgcgcgac atctccttca cgacgacgat cctccagggg ctcgacggcg gcctcatcct
    12901 catgccgaac tcaagcatca tcgcatccaa ggtcgtcaac tactcccgga acaggctgat
    12961 cgagatccgg gtctcgctct ccgtcccggt ctccgtcgat atcgcacggg tgcgcgatat
    13021 cgcgcttgcg gtggcgcatg acgactcccg ggtcatccgg actcccggcg ccgccggacg
    13081 gtcggccgta cacgacctct tcgacctccc ctacaaccgg cggctccgtt ccgatcgccc
    13141 cgatctcatc ggccttggac ccgccgtcca taccgtatcg gtcagcgacg gctggatcaa
    13201 gatggtggtg cgaatatgga cccagaagat accgcagaag gatgatatca cctcccgcta
    13261 ccttcgcgag atcctcgggc gccttgaggc cgaggatatc ccggtcaagg ccgaggcggc
    13321 ctgacggaag agattaatcc tcttatcgct cacttttcta cacatgcagg agagtgtccc
    13381 ggttctcatc ggcggcgtac ccctcgatgc gttcctcgcg ttcatcttcg ccttcatcgc
    13441 atccctcttc cttggcaacc tcgcctacat gctcctccgg cggctgctcg acggtcgcgc
    13501 ctcccgcgga aaggtcaaat gggcggcggc cattctccag tacggcatca ccgtcggcgg
    13561 ggtgtacgca agcgcccggt acttcctcga cttcgaactg acggccttct tcgcatcgct
    13621 cggtatactc ggtatcgccg tcgcgttctc gtcgcgccag atcatacaga acgccgttgc
    13681 gggggtcctg atcacgatca accgccctat ccagctcgag gagtgggtgg tcgtcagcgg
    13741 caggccggag accggcctct gcagggtgca ggacatctcc ttcacgacga cgatcctcca
    13801 ggccctcgac ggcggcctcg tcctcacgcc gaactcaagc atcatcacct caaaggtgat
    13861 caactactcc cgtgccggtc tgcttgaggt ctcggtcccg ctcgccgtcc cggtcaccac
    13921 cgagatcgca cgggtgcagg agatcgccgt cgcggccgcg cgcgaccatc ccctcatcct
    13981 cccccacgtc gagccggccg aacggtccgc cgtccagaac ctcttcgatc tcccctacat
    14041 cgggcgcctc tcatccgacc tcccccgccc cgacctccgg ctcttcgagc cgaaggtgca
    14101 ggtcgcgtcg atcaaggagg agtgggtcag gctgacggtg cggatctgga tccgccagat
    14161 ccccgggaag gaccggatcg tctcggagta cctcgatgcc gtcatcgggc acctctcggc
    14221 agagggtctc cttgaggcga agaaaccgga ggatgaccct gcaccggggt tccccggggc
    14281 cgcgtgacgg gccggggtgc cccatccgaa accgccaaaa agggggaaag atagaggaga
    14341 tgactatgat ggagaagacc cggagtacag ggaacggcat ccggttcacc ctcgaagaga
    14401 tcgccggagc cgtcggcgac ttcgggacca tcttccccat cctgctcggc gtcgccatcg
    14461 tcagcccgga cgtgaacgtc agccacttct tcttcttcct cgccgcctgg ttcatcatcg
    14521 ccgggctcta ctaccggctt cccatcccga tcgaaccgat gaaagcgatc ggcgccgtcg
    14581 tcatcgccgg cgggctctcc agcggcgaga tcgtggcctc aggcctcatc gtcggcgcgc
    14641 tctttctcct gctcggcctc gtcggcggta tgacctggct tggagaccgg ataccgaaaa
    14701 gcgtcatccg cggcgtccag gcagggcttg cgctcattct cctccggacg tcgctcggct
    14761 acatcgtcga cgacgttctc tttgcgatcg tctctatcgc gatcatcgtc gtcttcttca
    14821 tcgcatcggc gcgcacccgg ataccggata tatccgcgct gctggtcctc ctcatcgggc
    14881 ttgccgtcgg catcgccacc cagggaatgc cgccgttccg gctcataccc ctcccgccgc
    14941 tcatcgttcc ctcgctcccg gacttcgtcg ccggcatatg ggatcttgca ctcccgcaga
    15001 taccgctcac gatcgcaaac gccatcctcg cgacatcgct cctcacctac gacctcttcc
    15061 cgaagaaagg cgtcgacccc gaccggctct cgcggaccat aggcgcgatg aacctcatat
    15121 cgacaccgct tggagggttc cccatgtgcc acggcgccgg ggggcttgcc gccatgtacc
    15181 ggttcggcgc aaggaccggc ggggcgaaca tcgtcgccgg cctcttcatc ctcgtattcg
    15241 cggtcgtctt cgccccgccg gaggtgctga ccctcatacc gttcggtgtc tttggcgcgc
    15301 tcctggtctt tgtcgcgatc gagcttggaa agcacggcat aaagaccgag tcctacctgg
    15361 tcacgggagt catcgccgtc ctcgccctcg cgctcggcct caccatcgcg ttcatcgccg
    15421 gcatggccct tgcctatgcg ctggagtggt ggaaaaagga gcagggtgcc gggcaaaagc
    15481 ccggggcctg aagatacacc gggccaaggg ttcatgaggt ccggcacacg atagtgatgg
    15541 agtcccccat ggatgccatc accgcactcg gcctcatcgc cggttcgctc acgacgctct
    15601 cgtttgcgcc gcaggtcgtc cgggcctggc ggacgcggtc gaccgccgac ctctcccttg
    15661 cgacgctcat cgtcttcctt gccggcctcc tgctctggct cgcctacggc gtggtgagag
    15721 gggatcctgc catcgtcgcg gcgaacgccg tcaccgcggt cttcgtcggc ctgatcctct
    15781 cgatcaagac gaagcacgga tgacgtggat cgccgtcgcc ttgatagaga gggtgacggc
    15841 ggtccccgcg acgagcaaga gatcgcgtac cgaccgccgg gtcaggaccg cggtcagtac
    15901 gacgccggcg tccaccttca cctcggcgac ggggccgttc tccacgaccg agacgaccgt
    15961 cccggtcatc gtgttccggg cgctggaatg ccggtcgccg tcgacggcaa ggatgatatc
    16021 gtcggcccgg atgcagaggc tgacctcctc gcccggagcg gcgtcggtca tcgcctcaaa
    16081 cgtcctccct ccggcatcca ccgttgcatg cccatcctcg tttgccgtca cggtcccatc
    16141 gaggatgttc tcgataccga caaacgttgc gacatcacgg ctcctgggtg tgttcaggac
    16201 gacgccggcc ttcccttcgt ccacgaggta tccgtcgatg atcaccgcca tccgggtcgc
    16261 gaggaggtgc gcctcccggc gggagtggct cacctggacg atcgtgagcc cgtcctcccg
    16321 gtggagtcgg cggagatcgc cgatgaacct ctcctgcgtg atggggtcga gggccgagag
    16381 cggttcgtcg aggaggagga tgtcgggttt caccgcgatg gcccgggcaa gcgacacccg
    16441 ctgctgctcg ccgcccgaga gggttcccgg gtagcggtcg gcgaggtgag cgataccgaa
    16501 ccgctcgagg aggggaacaa cctcatcccg tgcctctttc tttcgcatcc cctgcacccg
    16561 gaggccgtag gagacgttgt cgatgacccg catgttcggg aagagcgagt agtcctggta
    16621 gacgagggcg acccttcgtt tctccggggg gagggtggtg atctcttcgt cccggaggag
    16681 cacccggccg ctctccggcc ggtggaggcc ggctatcgtc tcgaggagca ccgtctttcc
    16741 cgctcccgac ggcccgacga tgaagtagta atcgccccgg ccgatcgtca ggctgatctc
    16801 cttcaagcgg aacgagccga gtgcgagcga gacccgatca aactctatca tcgtacctcc
    16861 ccaggtgccg ggtcatccgc cggagaaggt aaaagacccc gaaactgatc aggatgaccg
    16921 tgaacgcgat gctcctgctc gcacggatgc cgtccgtcgt gaaggagtag tagatcagcg
    16981 tcgatatgat gaacggatag taggcgatca tgatgacgcc ggcaaactcc ccgatagccc
    17041 tcccccaggc gagtatggcc ccgctgtaca tgtgccggag gctcaagggg agcgagaccg
    17101 tccggaacat ggtgaaggtg ccggcgccga gcgtgcgggc gacgttctcc aggtggaccg
    17161 gcaccttctc aaacccctcc cgcatgctgt tgatgtagaa cggcgaggcg acgaagagca
    17221 tagcgacgat cgtcccgggg agcgcatcct caaacgcaag cccgatatcc gagagcggcg
    17281 cgccgagcca tccccggcgc atgaagagga ggtagacgag caggcccgcc acggtgtgcg
    17341 gcaggatgag cgggatatcg atgatgctct cgacgacgcc cttgaagcgg gaggggcggg
    17401 accgggcgag gatgtaggca agcggcgtcc cgaatacgat gaggaggagg acggcgctcg
    17461 cccccgcgcc gaacgtgagg aggatggact cgatcaccgt agacgacgtt gcgatcttga
    17521 tgaggtgggc cgggtcggcg agttccgcca tcgtgatgtt cgcgagcgcc agcaccgtga
    17581 tgccgacgag caacccgccg aggatgcaga acccgagagt gcaccagtcc gtataccgct
    17641 tgcgctctct cttccaccgg gtggcggcga ccggttctcc gtctgccgga agcgggtcgg
    17701 ttgagcgtgt ctgggtcttc atatccatgg aatcgctgat atctcctgtg cgcgcctcac
    17761 tgaatgcctg caggggaaaa aagaagaggg gagttcaaga tctcatcgca acgagcggct
    17821 tgaggacgtc agggacattg ccgtagccgt ccgcgggaac gatcgggggc tggccgtcgg
    17881 cgacgaggat ctcctggccg gtggtgtcga tcagcatctc gatgaatgcg acgccgaggt
    17941 cggggtgctc cgcgatcttc gggacggtta tgccgaagat gatgggggac ccggtgaaga
    18001 tggcgacgct gtcgcccttc ttcgtttcaa tctggacggt cgcgtaggta tcctcgaagt
    18061 ctatcgacga gaggtcgatc gcttcgggga gttcgatgta gcggaggtcg ttctggacgg
    18121 caacgctcct gtactcccag gcgtagtcaa gtccgccggc ctggaccatc tggacgagtt
    18181 cgacgctctt tggccggatc gtcaggacgg cgccgtcggg tttcgggtcg ccggcgtgga
    18241 tggtggagat gccgttctcc tcggtgacgg tgatgttgct gtgcttgctg acaaggttct
    18301 caaagatctg gtcgttcttg tagtggctct cggcaagctg gatcaccatc ggggtgcggt
    18361 aaccgcaggg atcggagttc gggtcggaga agccccaccg gacgccgtca cggccgagga
    18421 tctcgtacca gttatcggcg gtgatctcgc ttgcgtactt gctctcgttc gtataggtga
    18481 gcaccatcag gttctttgcg aacgtgagat accagtcggc gtgatcgggt atcatcatct
    18541 ccggtatgag gtagtaatcg gcggaggcga ggacatcggc gggtttgccg ttctcggtga
    18601 tcttcttgac gcaatcgatg ctgccggcag gctcgaggag caccgtggtg ccggggtact
    18661 tggcctcgaa ctctgctttc accttctcaa acggcccggt caggctcccg gcgtggaaga
    18721 tcttgacctg gatgttttcc gtgggtgctg ccgtcgatgc gggggtcgtg gtctgggcgc
    18781 ccgttccggg cgtcgagtcg gtgttcgtcg tccccgtgca gccgcagatc aggaccgcgg
    18841 cggcgacgat gagggcgata aaggcaacca tacgaatcga tttcatgaaa taatcttttt
    18901 tgggacaact gagttaaaat tatcctttaa ggcaagagtt aatcggttta gattaatcca
    18961 ggaggtccgg atagaacggc ggccggcaaa tagtgccccg atatgagttt acggcccatt
    19021 ttgcgggacc aggtggacca ccttcgccgg gagagagacc cagacctcca tcccgacgtg
    19081 gatctcgagg gcttccgccc tccgcgccgg catcagtacg acgagatcgc atccgcaatc
    19141 cacggtcacg ttcacgaacg gcgccatgga ctcgatagcc gtgacccttg ccgggaagag
    19201 gttctccgcc tcctcgtacc ccggttctcg ccggtgcagg gatatatcct cgccccggat
    19261 gaccacgaag acctcgtcgg cgggcgacgg tgtcgccgag atgatccgcc ggtcttttgc
    19321 cgccaccgtg gtgaggcccc cttgccggga gacgatccgg ccggggatga tgttctggac
    19381 gcccacgaac ccggcgatct ttctgctccc gggctcgtgg aagacggcat ggggtgagcc
    19441 cgcctgggcg accgtcccct cgatcatcac cgcaatgcgg tgggcgagcc gctgcccctg
    19501 cgccagatcg tgggtgctga tcaggacggt catcccggcc tcgcggttca ggtcggccac
    19561 gatccgctcg atggtggcgg tcgaacccgg atccaggttc gccgtgggtt cgtcgaggaa
    19621 gaggatctcg gggtccgtca cgagcacccg tgagagcgcc acccgctgct gctcgccgcc
    19681 cgagagatcg gtcgcccggc tcttgatgta gcgggagagc ccgacggcat cgagggcttc
    19741 cttcaccttc cggtcgatat cgctgccgga gaccccccgg taccggagcc ccatagcgac
    19801 gttgtcgtag accgacgcgt tgaagacgat cggcctctgg aagagcatcc ccatgcggcg
    19861 gcggaggtcg agccagcgac tgcgctccgc gaccgtatcg gtgccgaaga ccgagagctc
    19921 tccgccgctc ggcggctcga tcaggtcgag tatccgcaga agcgtgctct tccccgaccc
    19981 gctcgggccg atgagcccga ggatctctcc ttcctcgacc gagaggtcga cgtcgtgcag
    20041 gacctccagg ttgccgtata ctttcctgat gtcccttgcc tcaatgatca ccatgccatc
    20101 accgccgctg caccagggag agagcgatgt tcaccccgag agccaccgcg agaaggatga
    20161 tcccgagcgc gatcgatacc gagtagtttg ccatcgatgt gttgagcgcg atcgcggtgg
    20221 tgaggacgcg ggtggcgccc cggatgttgc cgccgatgat catcaccgta ccgacctcgg
    20281 atatcgcccg cccgaacccg agaaggacgc tggacatgac cgcgaaccgg gattccacga
    20341 tgatggtcac gatcgtctgg agcctgcttg cgccgagcgc ggtgatcgtg tagcgtttgt
    20401 cgcggtcgat accggagagg gccgagaccg tcagccccat gaggagcggt atgatgagga
    20461 ccgtctgggt gacgatcatg ccgccgggcg tgtagaggag gcggaggaac ccgaaagggc
    20521 cgatgtttga gaggaggagg aacatgacca ggcccacgat gacggtcggg agcgcgtaca
    20581 gcgtctgcag cgtcgagacg accgcatgct tcccccggaa ctcgtagaag tagatcagcg
    20641 ccccgagggg aagcgcgatg agcgcggcaa agaacgtggc ggtgagggag acgtagagcg
    20701 accggatggt gatctcgacc acctgcgggt cgagggagac gatgagccgg atggcctcga
    20761 tgaatccctc gacgatcggg ttcccgttca ccacgtctcc tcactcccct ctccccacat
    20821 ctccctggct acccttggtc tgctggcttc tacgcgggcg tgggcaccgg ctccgcacag
    20881 gtgcagttga acggcggtcc cgtgcactcg ggtgcgtaga gcggcacgaa gagcggtttt
    20941 ccaaactcga ccttcccgaa gtcaccgatc agctgcttcc cttcgtcgga tatgagccag
    21001 ttgatgaagt ccgcggcacc tctcgcgttc gtgttcgggt acttcgcggg gttgaccgcg
    21061 attgcgctgt agcggttcat gagctcggtg ctctcgctga tgatcggcac cagggcaagg
    21121 cggtttgcaa acgagagata ggttccctca tcggagagcg tgtaagcttt cttctcgtcc
    21181 gcgaggatca gggtgtcgcc catacctttg ccggcatcga cgtaccaggc tccggagtca
    21241 aggatctccg tcgtgtagtt gtagcccgcc tccttccaga gcgccttctc ctgggcgtgg
    21301 gtgcccgagt tgtcgccgcg ggagacgaag gccacgcccg gcgtgttgtt catgccggcg
    21361 atggcgatcg tcctgaaggc ttccaccgcg ctcatgttcc tgatcttcgc cgggtcgtcc
    21421 tccggtccga cgatgatgaa gtagttgtag gcgatgcacc gcgggtcgat gccgtagcct
    21481 tcgtcctcga actccttctc aagcgccggg gcgtggacga gcacgaggtc gacgtcgccg
    21541 cgccgagcgg tgtcgagcgc ctggcctgtg ccctgggcga tgaactggag gtccatcccg
    21601 gtctctctct cgtagacccg ctcgagctcc gcaagcagcc cggtgttctc aaggctcgtc
    21661 gtggtggcga tcttgagcac gtttgccccg gcagcgggtg cggtcgtcgt tacgttcggt
    21721 gttacgttct cgcccggcgc cgtccccgtg catcccgcca gaagagcgag gatgaagagg
    21781 atcgcgatgg tgccggccga tagaatggca acgttcatct tcatagcagc ccgttagcac
    21841 gaccaccgat cataaatagt tatcctcgca cccggagcat cctcatgacc gcaacggccc
    21901 ggggcgggga gaggtgtttc gcatacctca aagccgtctg tccccgcgag atcgggggat
    21961 ttctgccgcc ctccccgatc tcccggcaga ggccccccgc gcccgggtcc ccctgcggca
    22021 cggtacgaac gttcccggtt cacgctgtcg cgttcacctc tcccgatgag caggtggtgc
    22081 agttgaacgg cggttgcgaa cactcgggcg ggtagagcgg cacgaagagc ggctggccga
    22141 actctgcaac accaaagttc ccgatgaact ccttggtctc gttggaggtt atccagtcga
    22201 tgaacctgtt cgcctcgtcg atgttgacat tcgggttctt ctcggggttg accgcgatcg
    22261 cgctgtagcg gttcatgagc tcacccccct cggtgacgag ctgtaccagg ttcagctggc
    22321 tcttgaaggc aaggtaggtc ccgatgtcgg agagcgtgta ggcctccttc tggttcgcga
    22381 tggtcagggt ctcccccatg ccgctcccgg tctcgacata ccagtccccg gagtcctgga
    22441 tgtccgtcgt gtagttgtaa ccggccgcct tccagatctg ctgctcccgg ttgtgcgtgc
    22501 cggagttgtc gccgcgggag acgaaggcca cgcccggcgt gtcgttcatg ccggcgatgg
    22561 cgatcgtctt gaaggcctct tccggcgtca tgttcttgat cttcgccgga tcggaatccg
    22621 gcccgacgat caggaagttg ttgtaggcaa agcagtgcgg gttgatgccg tagccctgat
    22681 cgatgaactc ctgctcaagc cccggcgaat ggacgagcac gaggtcgacg tcgccgcgcc
    22741 gagcggtgtc gagcgcctgg cccgttccct gtgcggtgat ctgcacatcc acgccggaga
    22801 cgttctcgta catatcttca aggttgtcga ggaggccggt gtcatacagg ctcgtcgtcg
    22861 tcgctatccg gagtacgccc gggtttgccg cttgctgtgc cgtgacggag acggcacatg
    22921 cggcaagcag cactaccatg atgcccagga taggcatcca cgttctcgat cgcatgcggg
    22981 ccgggatggg ccgacattat atttatcggt ttgtataaaa attaacaaat aggatttgtc
    23041 aagtgccctc acatacctgc cgcccgtgag gcggtgttcg ccttcccggg gccggggtgt
    23101 cgaaacctta acgctctgcc ccgccgacct tttttgccat gtggcaggcg ctggagatcg
    23161 agacctggat cgccggacgg cgatcggaga tcgaccgggt gaggggcccc gtctctgaga
    23221 tcatagcgag ggtgcgatcg gaaggcgacg cggccctcat cgacctgacg aagagatttg
    23281 acggtatcga tctcgatgag atcgccgtct ccgacgaaga gcgggaggcg gcctacgacc
    23341 gggtggatgc acaactcatc gagagcctcg ccgaggcgga agcccggatc agccggttcc
    23401 acgagctgca gcgcgggcgc gacctctggc tgcaggagat ggagcccggg atcaccctcg
    23461 gcgtcaggac gacgccctta tcccgtatcg gtgcctacgt ccccggcggg cgggcggcat
    23521 acccctcgac ggcgctgatg tgcaccgtcc cggcaaaggt cgcgggggtc agggagatct
    23581 gctgctgcac cccgccgccg acaaacccga tcacgctcgt cgcgctcgat atcgccgggg
    23641 tcgacgagat ctaccgggtc ggcggcgccc aggcgatcgc ggcgatggcg ctcgggacgg
    23701 agacgatccc gcgggtcgag aagatcgtcg gcccggggaa cgtctacgtc acggcggcga
    23761 agatgcttct gcgcgacgag gtggagatcg attttccggc ggggccgagc gagatcgcta
    23821 tcctcgcgga cggcacggca aacccgacgt tcgttgcggc cgacatcctc gcccaggccg
    23881 agcacgaccc gaacgccgcc tgcgttctgg tgacgaccga tgcggtcctc gccgaccgtg
    23941 tcggcgccga ggtgaaacgg atggcggcgg gcgcggtgcg gcgcgagatc atcgatcggg
    24001 cacttagcca ctcgggctac atcgtcgccg ccgacatcga tgcggcggtg gagacggtcg
    24061 acgccgtcgc ccccgagcac ctctcgatcc aggtggccga tccgctcgcc gtcctcaccc
    24121 gggtcaggaa cgccgggtcg atcttcgtcg gcccctacgc ggcggttgcc tgcggcgatt
    24181 acgcctcggg gacgaaccac gtcctcccga ccgccgggta tgcccggctc tactccggtc
    24241 tcgacgtgaa ccacttctgc cggcgcgcaa ccgtccaggt gatcacgcgg gaggggctcg
    24301 aggcgatcgg cgacgtggtg gagacgatcg ccgatgcgga aggcctccac gctcacgcgg
    24361 agtcggtgcg ggtgaggagg agagggtagg tatcaagatc atctttcacc gtttgcccgc
    24421 acgaataaag tgaacccaat gagtgccctg tacgatgttt caggcccgaa aaccatagag
    24481 tgaactaccc cgccctgaag ggcggggtag ttcgctgacg catttttcga aaaaacgccg
    24541 ccctgtgcgg ggt
//
LOCUS       DAOSEE010000015        23729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_957_length_23729_cov_242.976768, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000015 DAOSEE010000000
VERSION     DAOSEE010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 23729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23729
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_957_length_23729_cov_242.976768"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..754
                     /locus_tag="PLI76_02180"
     CDS             245..754
                     /locus_tag="PLI76_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60870.1"
                     /translation="MLEANEYTRITQKLYSKSLILESPTEFHPVLHFYFIDALAHIDF
                     TLGILAYNYMSPRNIMSMEYMRWRIDEEKIGERAYFPGFINWLKEKHREKFDELPLLW
                     SGIYDRDDPAQYRSFRIVLNPDDKKAIPAEYLSTFIDEFFDPRFIKQLYQTSSLARLF
                     DEYAKSRSA"
     gene            766..1932
                     /locus_tag="PLI76_02185"
     CDS             766..1932
                     /locus_tag="PLI76_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOI60871.1"
                     /translation="MDVARLTSSLVRIKSENPPGSTADVVAFIGEYLDLLGVKNRIIS
                     HPGGRDNLITTEPDPRLLLCGHVDVVPAIPDDWTHDPYGGEIAGGYVWGRGTTDMKGG
                     CAALLIACRDLIESGVTPNVQFAFVCDEETGGPYGIRSLLAQDLLLPRECLIAEPTPA
                     MSPAVGQKGLYRIDLSFRGRPVHSSLYPLVGKSAVMAAFDLIGYLRKVHAHPFPVSED
                     LQPLVMQSARIFRDIFGIEGGDEILTRVMFNPGRIEGGEKANIVAEQCRMELDIRVPW
                     GCSLDDLRKSIAEHAPDAVIRETNVAAPSLTHPDARVVRTVSREVERVYGTVAPFLQW
                     AASDAKYLRDKGFDVAEYGPGDIPTLHAVDERVSIEQLEKAVDVYRGVIREYSG"
     gene            complement(2025..2978)
                     /locus_tag="PLI76_02190"
     CDS             complement(2025..2978)
                     /locus_tag="PLI76_02190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOI60872.1"
                     /translation="MKIHITRQGFIQLLLLSIGLVILAYFILQSGIIDNLWIITTISI
                     PLFILAFLLSIANIGSKVYRWKYLSRYYYQEISWYDASLVSISSLFFANITPGKVGDL
                     YKAYFMKRRYGMQFLDGVSMLFYERFFELTILFLVAAGIVMIQLRAVTVIALEFTLLV
                     LFLLLVFYYKADFFMGLAEKVLVRLPIERREQGLNLHIRKMPPSQIVVVFLITLFSLV
                     LEFARLWCVALAFGFLVNPIQLSIFMSLAFIIGLASQIPLGVGAMEGSLSYFITAMGV
                     PSVTAIAIVLVDRMISMYFALVLGFVFSKLSLDALKDAVKD"
     gene            3301..4032
                     /locus_tag="PLI76_02195"
     CDS             3301..4032
                     /locus_tag="PLI76_02195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOI60873.1"
                     /translation="MISIVIPLYNERENILTYRDELFPEIDRIADATGESFQYILVDD
                     GSRDDTYLLLQALARERSDVIAVTNGTNKGMGAAIKHGLQHSKGDLVVTMDSDLTFRP
                     EDVKALILAYQASHPDCVSGSPYLEQGLLEEVSFIRFLPSKVVNTLYRILLGADITCV
                     SPIFRLYRKDALDGIYIQSNNFDINAEILAKLLLGGRTVVEVPVVLHTRRYGESKINV
                     SKEIRNHVRLLTKIFKIRVFKGSWD"
     gene            4099..5178
                     /gene="wecB"
                     /locus_tag="PLI76_02200"
     CDS             4099..5178
                     /gene="wecB"
                     /locus_tag="PLI76_02200"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOI60874.1"
                     /translation="MHVAIVLGTRPEIVKMSPIIRYCEELGLDYSLIHTGQHYSYEMD
                     AAFFHDLELPKPGYKLNVGSGTHAEQTGKIMVGLERILIHDRPDVVLVQGDTNTVLGS
                     ALSAAKLNIDVGHVEAGLRSRDRTMPEEINRLLTDHIARYLFAPTELARSNLLQEGID
                     EEKIRVTGNTVVDAVLQNIEIAEQRSRIFRDLDLMPGGYFVLTAHRQENVDNREKLGN
                     LIAAMRELHSIYGLPILFPAHPRTLKRLDEFGLSVPEGIRILEPQGYLEFLLLERSAR
                     AILTDSGGIQEEACILHVPCITLRENTERPETLETGGNILAGTDPGRILQAVSTVLSA
                     EASWTNPFGDGRSGKKIVDILLGDR"
     gene            complement(5294..6820)
                     /locus_tag="PLI76_02205"
     CDS             complement(5294..6820)
                     /locus_tag="PLI76_02205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOI60875.1"
                     /translation="MAKKQGKRKKRDETETYDGGGNRNAGIPAPPPSQPSSSGTIISS
                     VRSSRHLQVLVGLTIAGLLLRFYNLGANSLWLDEASTLMFARQSLAGIWESTAGGEFN
                     PPLFYWLEHAMLLLGESEFVLRFLPALLGVLTIPVVYFVGKEFKDQNVGLIAAALLAF
                     SPFHIYYSQEARAYAPMLFFFSLALLFYVRANRSDEVRSWALFGLFSAVSFWMHFYAI
                     VPIAVLILHALVTRAGAIRHDILSAKNTAIAVAVFAVASLPLLIVTASLFLARTSSAP
                     TFGMQGLDVIYQTLFQVSGFNDLIMAAFIVLFLIGVAVMWRDDRNGAFFLIFMMSLPF
                     VASLILSSRMPMIPRHLIFLLPVYFIGIASAYPALHALVRNKKAVYAVVVAAALISTP
                     FLATYYTTPQKNDWRGFSAELGGMTGEGDLIVVLPPYMAQPLDYYYGNATDGTLELGA
                     NTGSDLEAIREQYPGRETFYVVTWDILAVDPTGDALNWLDKNAEFVGQRMGIYLFVSA
                     "
     gene            7031..7672
                     /locus_tag="PLI76_02210"
     CDS             7031..7672
                     /locus_tag="PLI76_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60876.1"
                     /translation="MTTVYKSFLAVAVMILILASAGCAELPPGGESGWSGILSPQKPQ
                     ETPEEVMEYDPGYLTRVTPYPTVTSSMTSPTLSNLPVVTPTPDTYVTLYNRTAWFVWT
                     TEAFAFDLTVPPLLIDFKVEPKMITREVRAKSDYKDRKDETYRQTFPSEDSWFKVTVR
                     DRNSGAIVAENGFGKLYSADARKQIYVGQSGNYQIDLTGNDVKVYVLIRAGGV"
     gene            8101..8172
                     /locus_tag="PLI76_02215"
     tRNA            8101..8172
                     /locus_tag="PLI76_02215"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8134..8136,aa:Arg,seq:gcg)
     gene            complement(8412..9548)
                     /locus_tag="PLI76_02220"
     CDS             complement(8412..9548)
                     /locus_tag="PLI76_02220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOI60877.1"
                     /translation="MHSEPPDDPDTLREKIIGLGETSLHKSYYPELQSRLAELERFRA
                     LLDQTHDAIFLIHAETGRLADVNASAGMYLDCSKKELLATPFINWISPQKKAVFDEFA
                     RRSCEQLPASRWTCIASLVSREGRETPVEITVRSVTFGDKQYIVAIARDITDRLQAER
                     ELKIKESALESSTNGILITDLAGTIMYANPGFASIFGYERGEMLAGRSLEIFFANPAI
                     GKKVTDLLRKNHAITEETIGVRNGGSPFHIQVSGSVVQNDAGQPLCIMLIVMDITERI
                     LIDQLKRETCAQLGKNIEQFAILGDHIRNPLQVIVGYAEMIDDPLAKMILDQSDRIDE
                     IITQLDRGWVESANVRQFLRRHGDDAEPRVETFIQRFSIKDWDS"
     gene            complement(9538..10686)
                     /locus_tag="PLI76_02225"
     CDS             complement(9538..10686)
                     /locus_tag="PLI76_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOI60878.1"
                     /translation="MIQRNQLDLRKFVVPEFICGYGALQLAGRYARNFGMKQVLLVTD
                     PGVRAAGWADAVGESLTEAGVPFTIFDAISPNPRSGQVMAGAEVYHSEECNGVVAVGG
                     GSPMDCAKGIGIVSSNMQDILTFEGVDRVPVPAPPLICIPTTAGSSADVSQFAIITDE
                     MRRRKIALVSKALVPDISLLDPRPLTTMPLELTVDTGMDALCHAIEAYVSNASSPMTD
                     LHALEAIRLIHSALPAVIEDPENLELRFSTLLASLHAGLAFSNASLGATHAMAHGLGG
                     VYDLPHGRCNALLLEHVIAANYEAAPDRYDRIGAIFDREPGEQTTPAGEIAAFRRSIG
                     ITATLTELGIRKDAIPTLARRAYADPCLVTNPRRLTCEDIERIYEHAL"
     gene            11002..11475
                     /locus_tag="PLI76_02230"
     CDS             11002..11475
                     /locus_tag="PLI76_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOI60879.1"
                     /translation="MAEKSRPPLPPFTYETAVQKVRMAEDAWNSRDPEKVSLAYSIDS
                     IWRNRSEFINGREEIVAFLQRKWAKELDYRLIKEVWAFHEARIAVRFAYEWHDDSNNW
                     FRSYGNENWEFDESGLMRRRYASINDLPIKEGERKYHWPLGRRPDDHPGLSDLGL"
     assembly_gap    11543..11642
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11643..12919)
                     /locus_tag="PLI76_02235"
     CDS             complement(<11643..12919)
                     /locus_tag="PLI76_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOI60880.1"
                     /translation="MDIKTERRDGILTFAIRGRLDGYGAGQLADAVEASLQADDRSVV
                     FDLAGMSYLSSAGIRILIATKKRLKERGGTLALAGIQDYPKSVLDMAGVTPIFSLYPD
                     LEVAVAACRRSKDSLSVINDLVRAPVVRDGVAYTVEPASTRSSSLLVTGDLDKMLHAR
                     LTESDIRAIPFSALPYSLGLGALGENVADALPLLGEMITLHGSVVWLPTDGNATPDFF
                     TPVKDAGKPCIYTGYNVTLYGPFHEIITIDAGDEGGIALSDIYRTIFDFARERKRDFS
                     GVVALAMWAVVDGVCSLGVKRSPIAASSPANGLPINAEENAKEWLEENDEPKYRGETM
                     VSFGFGIDYSACSGEHLSALTDRNRPKTQQLQLHNHGVIFRGVPWDASLDLTRQIERI
                     VTGAEFVDMRHLKDNTRIRRAKVGVAYISEIAV"
     gene            complement(13287..13769)
                     /locus_tag="PLI76_02240"
     CDS             complement(13287..13769)
                     /locus_tag="PLI76_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOI60881.1"
                     /translation="MACKCCQCGSCCSHMRIVHATIEERGNYTFIVHNAYNGDVKEVR
                     VDPDKIALFEDRSSIEELPDACPFLRFDEKTGKALCTVHLTRPDLCREYCCWRLLILD
                     SRGKRAGRVMYQTTFLPDDDELSRLWERVQPTLNGLCGTEWDGAVIDALTRAGYRVRR
                     "
     gene            complement(14152..16506)
                     /locus_tag="PLI76_02245"
     CDS             complement(14152..16506)
                     /locus_tag="PLI76_02245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOI60882.1"
                     /translation="MNRNLVAKYLDMLLISGQIDMQEMGTAKVYFLSHRVPISAMLDF
                     SSDMVVVLDDENRILRVNGPMLAVLGEDRQSVVGTDLDDSQNPFLIALRAHLPPPESR
                     GAPDQIIGVRCALQGKECHFRVKRIPTVFEDGGRGITLIIEDVTDQVANRERLKVSEA
                     RYRGIIEDQTEFIVRFMPDGAATFLNAAYARYLEESVEHLIGKPFLPCIHTEDAPIRD
                     QAILLLNRDHPVSIFECRIRHPSGRIRWNRWTVRALFDTSGHLSECQGVGRDITEKRE
                     AAAKVNDYIRGMEFLSQTCIAFMDMHEDHNIYDYVARQICALAPGLLVWIGIIDESDQ
                     NLVLKSVVGDPAAREVIGQLPGTHPAGTTFPINQTDRAELVRCKSLVRMPPHSRLLQI
                     QIPEELHSRIAKTTDSIDTYLMGLVTKGRLIGSVGISQKSGSVLPNKDLIEAFIRQAA
                     IAIDRRDASDTLKASLAREQEQVQNLQFLSRTAMKFIEMEDSEDIYRYIADRLYDLVP
                     ESIIGINSFDSGRRELTLRAVAGREEQVDTFWKALGINNPLGTSFPISKLPRSEVDFC
                     KNTLFEIPSVYDMFFHQIPQERCEQARDKLQFGSAYCMGFSRKGEIFGNIFIGLFRPG
                     TVTNREIIEAFINQASVALLRWFAREHHRESEGLFRVLVEQMPYPVVILGRDGYILGA
                     NGLAATLIGCRESAGLVGRPFRDCVEGGADLPPGLSRCLVSYEGSDLSCRLCLQPPGK
                     GACEVEAWGRMVRYNRKPALYVVLHPLSAEMENGSIPSTMPSSP"
     gene            16935..17279
                     /locus_tag="PLI76_02250"
     CDS             16935..17279
                     /locus_tag="PLI76_02250"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOI60883.1"
                     /translation="MQKYDFTGASNGADCLVRQAREKSMHGDHTAAVEYLMEAVDIEP
                     KHPEAFVLMGDCCDYLGQYEQAIANYDRALELDPYHADAWFNKGMSLRSIGRNIEAVQ
                     CIEKSIELYCGR"
     gene            17950..18537
                     /locus_tag="PLI76_02255"
     CDS             17950..18537
                     /locus_tag="PLI76_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60884.1"
                     /translation="MKVTEDGEQADDSALSIVLVFFDVYQKSIEVTIRRTMEEFLSSL
                     QSELSRPFDLSTMHQHNPLISQFKHKKEGFCPILHEIDGSIKIGITEIGIWDPVPPSR
                     FLYGVECDAHASLLSLYRFRQESTETDQLLQRLRKATIKIFGVACGLYHCDDPGCFLT
                     YHMTISDLDTGMYACRGCRDDFIRALQSLNSRIGA"
     gene            complement(18663..18851)
                     /locus_tag="PLI76_02260"
     CDS             complement(18663..18851)
                     /locus_tag="PLI76_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60885.1"
                     /translation="MTGAEREDPPESAGQFTGIILFTLLKIILFAGTKICRRSLVKLD
                     ADPYLDGRYLPARKEGMR"
     gene            18922..19350
                     /locus_tag="PLI76_02265"
     CDS             18922..19350
                     /locus_tag="PLI76_02265"
                     /inference="COORDINATES: protein motif:HMM:NF014080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOI60886.1"
                     /translation="MESDSSLEHIRRIQALIGRIEELADRIDRYQQGSNVQLNALREE
                     CDAVLAEAKQDYHALIVSAEMRGQDVTPDIALEDRRLKNTWLALYRHHGGATAEEISG
                     ELKRHRTTVSTYLNTLVLMGYAKKERVGHEVHYTAILNKD"
     gene            complement(19662..19746)
                     /locus_tag="PLI76_02270"
     tRNA            complement(19662..19746)
                     /locus_tag="PLI76_02270"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19708..19710),aa:Leu,seq:gag)
     gene            19918..20421
                     /locus_tag="PLI76_02275"
     CDS             19918..20421
                     /locus_tag="PLI76_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator of amino acid metabolism, contains ACT
                     domain protein"
                     /protein_id="HOI60887.1"
                     /translation="MWADISREFADSPSQTRVVRFLLENGFGINEEGRIVCNGVEIPA
                     THIGRAIETDRRVVDATARRILDNPDLREVFIRMRASPDLSRVAEALGLSVITLYPKD
                     AQQKGIVGAAVKVLVDHDLSIRQIFVTDPYLAEEPRLVMIVDGRRVPASVYEDLRALP
                     QVKRLII"
     gene            complement(20414..20896)
                     /locus_tag="PLI76_02280"
     CDS             complement(20414..20896)
                     /locus_tag="PLI76_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOI60888.1"
                     /translation="MREEDCIALLGRAGCSDDVVAHCRAVRDLALTYADGIADRDLIE
                     AGALLHDIGRGVTHDLHHAEAGGAICRAFGLDERIERIVERHIGAGLTADECSLLCLA
                     PRDCMPATLEEKIVAHADNLVKGIRAITMEERMMHAIALPRRQRKRIRRLGLEMELFR
                     "
     gene            complement(20889..21410)
                     /locus_tag="PLI76_02285"
     CDS             complement(20889..21410)
                     /locus_tag="PLI76_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor"
                     /protein_id="HOI60889.1"
                     /translation="MVSITELLNDPAINAYVLRLIGEEGVELLKRFPEGGEHSDEELA
                     EKTGVNLNTVRHTLYTLYEKRLAEYRRLKNTETGWLTYLWHLRLDRLHGVIEEEIRDV
                     LEHLDARLTYEEKNDFYMCKDCGIIYTFTDAAGWNFECPNCEEMLEHFDNELIANALR
                     RRVDRIKESLGSA"
     gene            complement(21517..22593)
                     /locus_tag="PLI76_02290"
     CDS             complement(21517..22593)
                     /locus_tag="PLI76_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOI60890.1"
                     /translation="MKGRVLVAGFATRHIAQSACRAGYTVYAVDHFCDRDLAWYTEDR
                     LSFDELADLPAKIAELAARHPVDALVVASGAEAVGTTIPLCGTPPAKVERFLDKLEIQ
                     RFFEGLDVPVPPLAGRDRYPAMVKPRRGAGGWRNAVAWAEEDLRRWEEAWPDVPYVAQ
                     QLVDGIPSSVSCIADGRRALAVAVNRQILRGEGECAHGFAGSVTPFSHPLAGEMVACA
                     ERIAAASGCTGSVGIDFMAGEKPWAIEINPRFQATLDTVEMATGENVFALHMDACRGV
                     LPATRPPARRVAVRRILFAERDMRLDADLSALAPRVADIPWPGTEFEEGQAVVSIFGC
                     AETEAAAFHDLERNIAAVRGLIGG"
     gene            complement(22590..23123)
                     /locus_tag="PLI76_02295"
     CDS             complement(22590..23123)
                     /locus_tag="PLI76_02295"
                     /EC_number="2.1.1.206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(56)-2'-O)-methyltransferase"
                     /protein_id="HOI60891.1"
                     /translation="MSGAPTVAVLRVGHRPERDQRVTTHVGLAARALGARGMYLAADD
                     PGVITSITDVASRWGGDFFVENGVSWRRCIGDWKAGGGKVAHLTMYGLRMTDVIDDIR
                     REERVLVVVGAEKVPGDIYGLADYNVSVTTQPHSEISSLALFLDHLFEGRELNREYPD
                     AKIRIEPAKAGKKTVER"
     gene            complement(23120..23401)
                     /locus_tag="PLI76_02300"
     CDS             complement(23120..23401)
                     /locus_tag="PLI76_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOI60892.1"
                     /translation="MREEDLDWTVYHCIPENSDITTPDLVAATGFEPGAVMASLERLE
                     HYLLIRRSGDGVRLLSLQESLIACQCRHTKDDLPFVIENGVVRATRRGD"
     gene            23529..>23729
                     /locus_tag="PLI76_02305"
     CDS             23529..>23729
                     /locus_tag="PLI76_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60893.1"
                     /translation="MADYMDLADIAERLFEASDDDDERLAGRLDTLDEETRRALLASD
                     LLNAYQVFYFWFRETPDDLAMER"
BASE COUNT         4920 a         6944 c         6966 g         4799 t
ORIGIN      
        1 accccgcccg cgtgggtcag agcgataccc atgatggttc gggacgaacc gtgcccgaga
       61 gaacttctgg cgtctattcc cggcaaagat agagagtgag cacgccctgg cccgactctc
      121 cccctccgtg gcttgccccg gacacccggc cgtagcgaag atcgccctcc ggccggcatc
      181 cggcgtttcc ggcctttctg cacagaagga gtactttttg taccaccctc cccaatagga
      241 gggtatgctg gaagcgaacg agtacacccg tatcacgcag aaactctaca gcaaatcgct
      301 gatcctggag agtccgactg agtttcaccc ggtcctgcac ttttacttca tcgatgccct
      361 cgcccacatc gactttaccc tcggcattct cgcctacaac tacatgtcgc cgagaaacat
      421 catgagcatg gagtatatgc ggtggcgtat agatgaggag aagatcggcg agcgggcata
      481 cttccccggg ttcatcaact ggctcaaaga aaagcaccgc gagaagttcg atgaacttcc
      541 actgctctgg tccggcatct acgaccgcga cgaccccgcc cagtaccgga gtttccggat
      601 cgtcctcaac cccgacgaca agaaggcgat cccggctgaa tacctctcga cgttcatcga
      661 cgagttcttt gacccgagat tcatcaaaca gctctatcag acctcatcgc ttgcccgcct
      721 ctttgacgaa tatgcgaaga gccggtctgc gtaaggagca cattcatgga cgtcgcccga
      781 ctcacgtcat cactcgtccg gatcaagagc gagaacccgc cggggagcac cgccgatgtg
      841 gtcgcgttca tcggcgagta cctcgacttg ctcggagtga agaaccgtat catatcgcat
      901 cccggcgggc gggacaacct catcacgacc gaaccggacc cccgcctcct cctctgcggc
      961 cacgtggatg tggtgccggc catccccgac gactggacac acgaccccta tggcggcgag
     1021 atcgccggcg gctacgtctg gggaagaggc acgaccgata tgaagggcgg gtgcgccgcg
     1081 ctcctcatcg cctgccggga tcttatcgag agcggcgtta ccccgaacgt ccagttcgcc
     1141 ttcgtctgcg acgaggagac cggcggcccg tacgggatcc ggtcgctgct tgcacaggat
     1201 ctccttctgc cccgcgaatg cctgatagcc gaaccgacgc cggcaatgag cccggcggtc
     1261 ggccagaaag gcctctaccg gatcgacctc tccttccgcg gcagacccgt ccacagttcc
     1321 ctctaccccc tggtcgggaa gagcgccgtc atggcggcgt tcgacctgat cgggtattta
     1381 cgaaaggtcc acgcgcaccc gttcccggtc agcgaagacc tgcagccgct cgttatgcag
     1441 tcggcacgga tctttcgcga tatcttcggt atcgagggag gggacgagat cctcacgagg
     1501 gtgatgttca acccgggaag gatcgagggc ggcgaaaagg cgaacatcgt ggcggaacag
     1561 tgccggatgg agctggatat ccgggtgccg tgggggtgct ctcttgacga cctccggaag
     1621 agcatcgccg aacacgcccc ggatgccgtg atacgcgaga cgaatgtcgc ggcgccgagc
     1681 ctgacccacc cggatgcccg ggtagtccgg acggtctccc gtgaagtgga acgggtctac
     1741 gggacggtag cgccgttcct ccagtgggcc gcaagcgacg cgaagtacct ccgcgataag
     1801 gggttcgacg tcgcggagta cgggccgggg gatatcccga cgctgcacgc ggtggacgaa
     1861 cgggtctcca tcgaacagct tgagaaggcg gtggatgtct accggggggt tatccgggag
     1921 tactcggggt gacccctctc ctgccggcac aaggtagcta tcgatcccgt tctcccccat
     1981 tttatccatc ccgggcggca agaggagggg cggatctgat ccgctcaatc cttcacggca
     2041 tccttgaggg catcgagaga gagttttgag aagacgaagc ccagcaccag ggcgaagtac
     2101 atcgagatca tgcgatcgac gaggacgata gcgatcgccg tgaccgaagg aacgcccatg
     2161 gccgtgatga agtagctcag cgaaccctcc atcgccccca cgccgagggg tatctgcgag
     2221 gcaagcccga tgatgaaggc gaggctcatg aagatcgata gctggatcgg gtttacgagg
     2281 aacccgaatg ccagcgccac gcaccagagc cgggcaaact ccaggaccag tgagaacagc
     2341 gttatcagga agaccaccac gatctgagag ggtggcatct tccggatgtg caggttgagt
     2401 ccctgttctc tcctctcgat aggcaacctg accagaacct tctccgccag tcccatgaag
     2461 aaatctgctt tgtagtagaa gaccagcaac aggaacagca cgagcagggt gaactcgagc
     2521 gcaattaccg tcacggccct gagctggatc atgacgatgc cggcggcaac caggaagagg
     2581 atggttaact cgaagaagcg ctcataaaag agcatggata ctccatccag gaactgcatg
     2641 ccgtacctgc gtttcatgaa gtatgctttg tagaggtcgc cgacctttcc aggggtgata
     2701 tttgcaaaga acagactgga tatactcacc agggaggcat cgtaccatga tatctcctga
     2761 taatagtacc tgctcaggta tttccagcga tagaccttcg accctatgtt tgctatggag
     2821 agcaagaatg ccaggataaa gaggggaatg ctgatcgttg tgatgatcca gaggttgtcg
     2881 atgatgcctg actgcaggat gaagtatgcc agtatcacaa gaccgatcga gaggagcagc
     2941 aactgaatga atccctggcg ggttatgtga atcttcatgg catcctcagc gccccggggc
     3001 cccccggagg tcatcccctg atcccccgct ctccagtcgg cagcacctca tatcggaaaa
     3061 ccacatcccc cccgggggga gcgtcgggat gatctctccg gagaccggac agttcaacgg
     3121 gcattggtcg tatatagcgt atgcctcttt gatacattaa gccagcgaag atatccgctg
     3181 agaaccaagt ttacatacac ggcggcgcta ttccatgggg aatccatcca atcgcccagg
     3241 gtttatcagg tctggatgcc aatgataatt tatccgtaca gttcaacggg agaatatctg
     3301 atgatttcca tagtcattcc gctctacaac gagagagaaa acatactgac gtacagggac
     3361 gagctttttc cggagatcga ccgtattgcg gatgccacgg gagagtcctt ccagtatatt
     3421 ctggttgacg atgggagtcg tgacgataca tacctgttgc tgcaggcact tgctcgcgaa
     3481 cgatccgacg ttatcgcggt gaccaacgga acgaataaag gcatgggagc ggcgataaag
     3541 cacggtctgc agcattccaa aggggatctt gtcgtaacca tggactcgga tctcacgttt
     3601 cgccccgagg atgtcaaagc ccttatcctg gcataccagg cgtcgcaccc ggactgtgtc
     3661 tcaggatctc cgtacctcga gcagggatta ctggaggaag tcagttttat ccggtttctg
     3721 ccgagcaaag tggtgaacac gctgtaccgg atcctgctcg gcgcagatat cacctgtgta
     3781 agccccatct ttcggcttta caggaaggat gcccttgacg gaatatacat acagagcaac
     3841 aactttgata tcaacgccga gatactggct aaactgctcc tcggcggcag aacggtagta
     3901 gaagtcccgg tcgtactcca cacgcgcagg tacggcgaat caaagatcaa cgtcagcaaa
     3961 gagatccgga accatgtgcg gctcctgacc aaaatcttca agattcgagt cttcaaaggg
     4021 agctgggact gatgcggatc ggatcgtcca ttactctttc cgcacaacaa agatcgtgac
     4081 cgccagaggt gatagagtat gcatgttgct attgttcttg gaacccgccc cgagatcgtc
     4141 aagatgtctc ccatcatccg gtattgcgaa gaactcggtc tggactactc gctcatccat
     4201 accggccagc actactcgta cgagatggac gcagcgttct tccacgacct cgaactgccg
     4261 aaacccggat ataagctgaa tgtaggatcg gggacgcatg ccgagcagac cggtaagata
     4321 atggtcggcc tggagcgtat ccttatccac gatagacccg acgttgtgct cgtgcagggc
     4381 gacaccaaca cggtcctggg ttcggcgctg tccgctgcaa agttgaacat cgatgtcggg
     4441 catgtggagg ccgggctgcg gagccgggac aggacgatgc cggaagagat aaaccgtctt
     4501 ctgacggacc atatcgcgag atacctgttt gcacccacag agctggcaag gagcaacctt
     4561 ctccaggaag gcatcgacga ggagaagatc cgggtaaccg gaaacaccgt cgtcgatgcg
     4621 gtccttcaga atatcgagat cgcagagcag cgatcccgta tctttcgcga tctcgatctt
     4681 atgccgggcg gctacttcgt actcaccgcc cacaggcagg agaacgtcga caaccgtgaa
     4741 aaactgggga acctgatcgc cgcaatgcgg gaactccatt cgatctacgg gctcccgata
     4801 ctcttccccg cgcacccgcg cacgttgaag cgcctcgatg agttcggcct gtcggtaccg
     4861 gagggcatac gtatactcga accgcagggg tacctggagt tcctgctcct ggagcgatcc
     4921 gcaagggcaa tcctgacaga ctccggcggc attcaggaag aggcatgcat actgcatgtt
     4981 ccctgcataa cgttgcggga gaacaccgag cggcccgaga ctcttgagac gggagggaac
     5041 atcctcgcag ggacagatcc cgggcgaatt ctgcaggctg tgtcaaccgt actctcggct
     5101 gaggcgtcat ggaccaatcc gttcggtgat ggacggtcgg gaaaaaagat tgtagatata
     5161 ttgcttggcg atcggtagca tcgcctctcc cgatctatct tctgttttca accgccgctt
     5221 cctgcctgcc ggcaatatat cttcgccagg aggcgatcgc cggcaggccc ggtcggtgca
     5281 gcaaatatcc cttttaggcc gacacgaaga ggtagatccc catacgttgc ccgacaaact
     5341 ccgcgttctt atccagccag ttcagcgcgt cgccggtggg gtccacggcg aggatatccc
     5401 atgtcaccac gtagaacgtc tctctgcccg gatactgttc ccgtatcgcc tcaaggtcac
     5461 ttccggtatt tgccccgagc tcgagggtcc cgtcggtcgc gttgccgtag tagtagtcga
     5521 gcggttgcgc catgtagggc ggcagcacga cgatgaggtc gccttctccc gtcatcccgc
     5581 cgagttccgc ggagaatccc cgccaatcgt tcttctgggg cgtcgtgtag taggtcgcaa
     5641 ggaagggcgt gctgatgagg gccgccgcca cgacgacggc gtagaccgcc ttcttattcc
     5701 ggacgagggc atggagtgcg ggatacgccg acgctatccc gatgaagtag accgggagga
     5761 ggaagatgag gtggcgcggg atcatcggca tcctcgacga gaggatgagg cttgccacga
     5821 acgggaggct catcatgaag atgaggaaga acgctccgtt tcggtcgtcg cgccacatga
     5881 cggcgacacc tatcaggaag agaacgatga acgccgccat gatgagatcg ttgaaccccg
     5941 atacctggaa gagggtctgg tagatgacgt cgaggccctg catgccgaac gtcggggcgg
     6001 acgacgtcct ggcgagaaag aggctggccg tgacgatgag gagaggaaga cttgccacgg
     6061 cgaacacggc gaccgctatg gccgtattct tcgcgctcag gatgtcgtgc cggatcgcgc
     6121 cggcgcgggt gacgagggca tggaggatga ggaccgcgat gggcacgatg gcgtagaagt
     6181 gcatccagaa ggagaccgcg gagaagagcc cgaagagggc ccatgagcgg acctcgtccg
     6241 accggttcgc cctcacgtag aagaggagcg cgagggagaa gaagaagagc atcggcgcgt
     6301 atgcccgggc ttcctgggag tagtagatgt ggaaaggcga gaatgcgagg agggcggcgg
     6361 cgatgagccc gacgttctgg tctttgaact cctttccgac gaagtagacg accggtatcg
     6421 tcaggacgcc gaggagcgcg gggaggaagc ggagcacgaa ctcgctctct ccgaggagga
     6481 gcatcgcgtg ctccagccag tagaagagcg gcgggttgaa ctcgcctccc gcggtgctct
     6541 cccagatgcc ggcgagcgac tgccgggcga acatgagcgt cgaggcctcg tcgagccaga
     6601 gcgagttcgc tccgaggttg tagaaccgga ggaggagccc ggcgatcgtc agtccgacga
     6661 ggacctgcag gtgtctgctg gatcggacgg acgatatgat cgtaccagaa gaagaaggtt
     6721 gcgatggcgg cggggcaggg atgccggcgt tacgattccc tccgccgtcg tacgtctccg
     6781 tctcgtcgcg ttttttccgt tttccctgct tcttggccat atgtaatcat cagacattga
     6841 gcgcaaatac agatatacat taccggatgg cccggggagc gcctgcgggc gagcccggcg
     6901 ttacggagtt tgcacgcccc ggtcaggatc cggcatgata tttcccgatg ggatatatgc
     6961 ctggcgcata ctgtccaccg gaggagccgg gggtaaaagc ataaataccc ctttgcataa
     7021 ttcccctacg gtgaccacag tgtataaatc gttccttgca gttgccgtga tgatcctgat
     7081 cctcgcatcc gccgggtgcg cagagctgcc tcccggcggc gaatccggtt ggtccggcat
     7141 cctttcaccg caaaaaccgc aggagacgcc ggaagaggtg atggaatacg atccggggta
     7201 cctgacgcgg gtgaccccgt atcccacggt aacaagcagt atgaccagcc ccacgctcag
     7261 caacctgccg gtagtcacgc cgacgcccga tacgtacgtg accctctaca accggacggc
     7321 ctggtttgtc tggacgacgg aggccttcgc ctttgacctg acggtgcctc cgctcctgat
     7381 cgactttaag gtggaaccga agatgatcac gcgggaggtg cgtgcaaaga gcgactacaa
     7441 ggacaggaag gatgagacct acaggcagac cttcccaagc gaagattcat ggtttaaggt
     7501 gaccgtgagg gaccgcaatt ccggcgcgat tgtggccgag aacgggtttg gcaagctcta
     7561 cagcgcagat gcccgtaagc agatctacgt gggccagtcc gggaactacc agatcgatct
     7621 caccggaaac gacgtgaagg tgtatgtgct gatacgtgca gggggcgtgt agcggagggt
     7681 tgcacctgat gagtcaggtg aaccgctgct gcctacgtga gcgtgatagc gagctgcaag
     7741 cacccggaca ggctttgcgt gagctcgatg atacgggcga tgaaacagcc tctcgcgatg
     7801 aactgcgccg cgaaaggggg ttttcggtga tcgcgcgcga tacccggtgt gggtagcgat
     7861 acaatctcat gcgaaagacg tgttatcaca ataggaggcc gcacacagtt agcccggtct
     7921 tcccggggcg gcgatccgcc tcccgcaccg ggacctccgc catgccgata gaaataaccc
     7981 gacaaacccg ccccttatac cgaaccctgc agtctctact cgatatggtg catcttccgg
     8041 gtgagcaatc ctttttaagg aggagagcct agtaattagg ggtaagacac ttattctcgt
     8101 gtcccggtag ggtagtggat atcctagaag cctgcggagc ttttgacccg ggttcaaatc
     8161 ccggccgggg cgcttcttgt tttggtcttt cggccccctc agatttatgg tcgtcagtgc
     8221 cggcgcgcca tttgcgaccc cggtatgcat ccatgcgggt tatccggcct tctcgccccg
     8281 gatgcgtttc ctgcctccgc ctgctgctta tccgtgaatg ccgcgcaggg tgaacgtatc
     8341 cgccggaaga tccggtttaa ccgcgcgggg tccaggcccg ggggatgccg ttgcaccgtg
     8401 gagtccggta atcaggaatc ccagtctttt atggagaatc tctggatgaa cgtctccacc
     8461 cggggctccg cgtcgtctcc gtgcctgcgc aggaactgcc tgacgtttgc cgactcgacc
     8521 catccccggt caagttgagt gatgatctca tcgatccggt ctgactggtc gaggatcatc
     8581 ttcgcgagcg gatcgtcgat catctccgcg tatccgacaa tcacctgcag cgggttcctg
     8641 atatgatctc cgaggatggc aaactgctcg atgttcttcc cgagctgcgc gcatgtctcc
     8701 cgtttcagct gatcgatgag tattcgctcg gtgatatcca tgacaatcag catgatgcag
     8761 agcggttgcc cggcgtcgtt ctgcacgacg ctgcccgaga cctggatgtg gaacggggat
     8821 ccgccgttac gcaccccgat tgtctcctcc gtgatcgcgt gatttttacg gagaagatcc
     8881 gtcacttttt tgccgatggc agggtttgca aagaatatct caaggctcct ccccgcgagc
     8941 atctcccccc gctcgtaccc gaagatcgag gcaaagcccg ggttggcata catgatcgtc
     9001 ccggcaagat cggtgatgag gatcccgttg gtcgatgact caagagcact ctccttgatc
     9061 ttgagttctc tctcggcctg gagccggtcg gtgatatccc gtgcaatcgc tacgatatac
     9121 tgcttgtcgc caaaagtgac ggatcggacg gtgatctcca ccggggtctc acggccctcc
     9181 cgcgagacca gcgacgcgat gcaggtccag cgggacgcgg gcaattgttc gcaactcctc
     9241 ctggcgaact catcaaatac ggccttcttc tgcggggaga tccagttgat gaacggtgtc
     9301 gcaagcagtt ctttctttga gcaatccagg tacattcccg cagatgcgtt aacatccgca
     9361 agtctcccgg tctctgcgtg gatcaggaat atcgcgtcgt gcgtctggtc gagcagggcc
     9421 ctgaaccgtt cgagttccgc aagccggctc tggagctccg ggtagtaact cttgtgcagg
     9481 gatgtctctc caaggccgat gatcttctcc cggagcgtat ccgggtcgtc gggaggttca
     9541 gagtgcatgc tcatagatcc gctcgatatc ttcgcaggtg agcctgcgcg ggttcgtcac
     9601 cagacaggga tcggcgtatg ctctccgggc aagggtggga atagcatcct tgcggatacc
     9661 gagctcggtg agggtcgccg tgatgccgat cgacctccgg aacgccgcta tctccccggc
     9721 aggggtcgtc tgttcgcccg gctcccggtc aaagatcgct cctatccggt cgtagcggtc
     9781 cggcgcggct tcgtagttgg ccgcaatcac gtgctcaagg aggagcgcgt tgcaccgacc
     9841 gtgggggagg tcatagaccc ctccaagacc gtgcgccatc gcgtgcgtgg caccgaggct
     9901 tgcattcgag aacgcaagtc cggcgtgaag gctggcaagg agcgtcgaaa accgcagttc
     9961 caggttttcg gggtcttcga tgaccgcggg cagcgcgctg tggatgagcc gtatcgcctc
    10021 gagggcgtgc aggtcggtca tcggggaact ggcattcgat acataggcct cgatcgcgtg
    10081 gcagagagca tccatgccgg tatccacggt cagttccagg ggcatcgtcg tcagcggtcg
    10141 tgggtcgagc agggagatgt cgggaaccag cgctttcgag acgagggcaa tcttacgccg
    10201 tctcatctca tccgtgatga ttgcgaactg agagacgtcg gcggaacttc cggcggtcgt
    10261 cgggatgcag atgagcggcg gtgcgggcac cggcacccgg tcgacgccct caaacgtgag
    10321 gatatcctgc atgttggaac tcacgatccc gatgcccttg gcgcagtcca tcgggctacc
    10381 cccgccgacg gcgacgacac cgttgcattc ttcggaatga tacacctccg cgcccgccat
    10441 cacctgccct gagcggggat ttggactgat ggcatcaaag atggtgaacg gtacccccgc
    10501 ctccgtcagg ctctctccga cggcatccgc ccagcctgcg gcccggacgc cgggatcggt
    10561 gacgaggagc acctgtttca taccgaagtt acgcgcatac cggcctgcaa gttgcaatgc
    10621 tccatatccg cagataaact caggaacgac gaattttctc agatcaagct gatttctctg
    10681 gatcattatc cggcacccgg ttgtgaatcg attcctgact gcaagaccca atagtattcc
    10741 ggtggctgaa atgagtccgt tgttcaccag gtacggaact atatccatat atactttggc
    10801 gaagtaacca gggggttatc ctggtcgatt aaagggtact gctggataat ggtcagggaa
    10861 tttgtgaaaa tcgtaaaatt ttgcgcaata ggagatgtag cgaaccatga tctcctgcac
    10921 cacttcgatc ggtgtcatgc catcccgtca tcgtgggtat cgaccgcacc tttatcatgt
    10981 tgcatgaggt agaaggggcg catggccgag aagagtcgcc ctccactgcc gccgttcacg
    11041 tatgagacgg cggtgcagaa ggtccgtatg gctgaggatg cctggaactc ccgtgacccc
    11101 gagaaggtct cgcttgcgta cagcatcgac agcatctggc gcaacagatc ggagttcatc
    11161 aacggtcgcg aggagattgt cgcattcctg cagcgaaaat gggcaaaaga gcttgactac
    11221 cgcctgatca aggaggtatg ggcgttccac gaggcgcgta tcgccgtacg gttcgcctac
    11281 gaatggcacg acgattcgaa caactggttc cggtcttacg ggaatgagaa ctgggagttc
    11341 gatgagtccg gcctgatgcg ccgccgctac gcaagcatca acgatctccc gatcaaagag
    11401 ggggagcgga agtatcactg gccgctcggg cggcgccccg acgatcaccc cgggttgtcc
    11461 gacctcgggc tataaacctc ccgcccacgg gcaggaggag cggggatatc ccccgcctct
    11521 tttatctccg gaggtatccg gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11641 nntcgacggc gatctccgat atgtaggcca cgcccacctt cgcccgccgg atacgggtgt
    11701 tgtccttgag gtggcgcata tcgacgaact cggccccggt tacgatccgc tcgatctgcc
    11761 gcgtgaggtc gagggacgca tcccacggca ccccccggaa gatgacgccg tggttgtgga
    11821 gttgcagctg ctgcgtcttc ggacggttcc ggtcggtgag ggccgatagg tgctcgccgc
    11881 tgcaggcgga gtagtctata ccgaacccga agctgaccat cgtctccccg cggtacttcg
    11941 gttcgtcatt ctcctcgagc cactcttttg cgttctcctc cgcgttgatg gggaggccgt
    12001 ttgccgggga cgacgcggcg atgggcgaac gcttcacccc caggctgcag accccgtcga
    12061 cgacggccca catggcaaga gcgacgacgc cggaaaaatc gcgctttcgc tcccgggcaa
    12121 agtcaaagat cgtgcggtat atgtcggaga gcgcgatgcc gccctcatcc cccgcgtcga
    12181 tcgtgatgat ctcgtgaaac ggcccataaa gcgtcacgtt gtaccctgtg tagatgcagg
    12241 gctttccggc atccttcacc ggcgtgaaga agtccggtgt ggcgttcccg tcggtcggca
    12301 gccataccac cgagccgtgc agcgtgatca tctccccgag gagcgggagc gcatccgcca
    12361 cgttctctcc gagcgcaccg agcccgagcg agtacgggag agccgagaac ggaatcgcac
    12421 ggatgtcgct ctcggtcagg cgggcatgca gcatcttatc caggtcgccg gtgaccagga
    12481 gcgacgaact ccgcgtggag gccggctcga cggtataggc aacgccgtcc ctcacgaccg
    12541 gcgcccggac aaggtcgttg atgaccgaga ggctgtcttt tgaccgcctg caggcggcga
    12601 cggccacctc aagatccggg tagagagaga agatcggcgt cacgccggcc atatcgagga
    12661 cgctttttgg gtaatcctgt attcccgcga gggccagcgt gccgcctcgt tccttcaggc
    12721 gcttctttgt ggcgatgaga atccggatgc ctgcactgct caggtaactc atgccggcaa
    12781 ggtcgaagac gaccgaccgg tcgtcggcct gcagggaagc ctccacggca tctgcaagct
    12841 gccctgcgcc gtagccgtcg agccttccgc ggatcgcaaa cgtcaggatc ccgtctctcc
    12901 tctcggtctt gatgtccatg tgtatcccgg tatagatatc aggctgttgt cagttaatct
    12961 ttctcaaatt cgttccccca tcggcgtgtc gtgccgggga agactttgtc ggggatcgcc
    13021 ggaacggccc ggcaccgggg atctcacata accgtgctct tgagttctcc ggcgtttacc
    13081 gtcgggattg ccccccggtt gcaaccgaag gaaagacgcc ctccgaccac ctcgtctgcg
    13141 aacggggacg ggcgatgtgc tacggtgccg ggttggggcg ttcgcgcaat cggttgccgt
    13201 cgggctcgac gatacgatgg ggccgagggg agcccgttcc ggagaggaac aggggcatac
    13261 ccctcttttt cggagtctcc cggcagctac cggcgaacac ggtagccggc tctcgtgaga
    13321 gcatcgatga cggcgccatc ccattccgtg ccgcagagcc cgttcaacgt cggctgcacc
    13381 cgctcccaga gccggctgag ttcgtcatcg tccgggagga acgttgtctg atacatcaca
    13441 cgtccggccc gctttccccg cgagtcgagg atgaggaggc gccagcagca gtactcgcgg
    13501 cagaggtcgg ggcgtgtcag gtgaaccgtg cacaacgcct ttcccgtctt ttcgtcgaat
    13561 cgcagaaaag ggcatgcatc cggcagctcc tcgatactgc tcctgtcctc gaagagggcg
    13621 atcttgtccg ggtcgacccg cacctccttc acatccccgt tataggcgtt gtgcacgatg
    13681 aaggtgtagt tgccacgctc ttcgatagtc gcgtgaacga tccgcatgtg gctgcagcag
    13741 ctgccgcact gacagcactt acacgccacg gggaccgcct ccccaaagac cgggcggttg
    13801 gtgtatcggc atgctcatta tctggtacta cgcatcgcaa tgatgaaata cctgccggaa
    13861 aaccgcccgg accacacctg caaccgggcg gttccggagc cgccggcgac ggttgccggc
    13921 cgcccggctc tcggcgtagc agtgccggtc acggcgcaga agaatcttga ccgcccggga
    13981 ggagaggcac ggccctcaat gcagagcggg ggggcggcga cggttgccgg ccgcccggca
    14041 gcgcggagag acgaaacgtg ccaggtgatc ccgctctgcc tgcgtaccgc ggaaggtcga
    14101 ggagatggcg atgcgggagg catggtaacg aggtcttgac acgtctgtag actaagggct
    14161 gctcggcata gtagacggga tgctcccgtt ctccatttct gctgagagcg gatgcaaaac
    14221 cacatacagt gcaggtttcc tgttatatcg aaccatccgt ccccaggcct ctacttcaca
    14281 ggctcctttc ccgggaggtt gcagacatag ccggcaggac agatcgctgc cttcgtagga
    14341 aacgaggcac cgtgacaatc ccgggggaag gtccgctcca ccttccacgc agtctcgaaa
    14401 cggcctcccg acaagccctg ctgattcacg gcatccgata agagtggcag caagcccgtt
    14461 tgcaccaaga atgtatccgt cccggccgag aatcacgaca gggtacggca tctgttcaac
    14521 aagcacacga aagagtcctt cactctcgcg gtggtgttct ctcgcaaacc accgcaggag
    14581 ggcgacagag gcctgattga taaatgcctc gatgatctcc cggttcgtaa cagtgcccgg
    14641 ccggaagagc ccgataaaga tgttaccaaa aatttcaccc ttacgggaga atcccatgca
    14701 ataagcgctg ccgaattgta acttatcccg ggcctgctcg cagcgctcct gcgggatttg
    14761 atggaagaac atatcgtaaa ccgatgggat ctcaaagagc gtgtttttac agaagtcaac
    14821 ctcagatcgg ggcagtttac tgatgggaaa cgacgtcccg agcggattgt taatcccgag
    14881 agccttccag aaggtatcga cctgctcttc tcttcccgca acagctcgaa gcgtaagttc
    14941 cctccgcccc gaatcgaacg aattgatgcc gatgatactc tccgggacaa gatcgtagag
    15001 gcgatccgca atatagcgat agatatcctc cgagtcctcc atctcgatga atttcatcgc
    15061 agtccgggag aggaactgca ggttctgaac ctgctcctgt tcgcgcgcca ggctcgcttt
    15121 gagggtatcg ctggcatccc tcctatcgat cgcaatcgca gcctgccgga tgaacgcctc
    15181 gatgagatcc ttattcggca gtacagaacc gcttttctga cttatcccga cgcttccgat
    15241 gagcctgccc ttggtgacga gccccatcag gtaggtgtcg atgctgtctg ttgtctttgc
    15301 aatccggctg tggagctctt cagggatctg tatctgcaga agtcgggagt gtggaggcat
    15361 cctgacgagg cttttacacc ggacaagttc cgccctatct gtctggttaa taggaaacgt
    15421 cgtccccgca gggtgcgttc ccgggagttg cccgataacc tcccgtgcgg ccggatcccc
    15481 gacaacgctt ttaaggacga gattctggtc ggattcatcg atgataccga tccataccag
    15541 tagcccgggg gcaagcgcgc agatctgtcg tgcgacgtaa tcgtagatgt tatgatcttc
    15601 gtgcatgtcc atgaaggcaa tacaggtctg tgagaggaac tccatccccc ggatataatc
    15661 gttgaccttc gctgcagctt cgcgtttctc ggtgatatcc ctgcccacgc cctggcactc
    15721 ggagaggtgc ccggacgtat caaagagcgc gcgtaccgtc catctattcc agcgaatccg
    15781 ccctgaagga tgacggatcc ggcattcgaa tatcgagaca ggatggtcac gattcagtaa
    15841 tagaattgct tgatctctga taggagcatc ttcggtatgg atacagggaa gaaagggttt
    15901 cccgataagg tgctccacgc tctcctcaag ataccgggca tatgcagcat tcagaaacgt
    15961 agcagcgccg tccggcataa accggacgat gaactcggtc tggtcttcga tgataccccg
    16021 gtaccgggct tcactcacct tcagcctttc ccgattggca acctgatcgg tgacatcctc
    16081 gatgatcagc gtgatgcccc ggcctccgtc ttcaaatacc gtcggtatcc gtttcacgcg
    16141 gaagtgacac tcttttccct gcagggcgca acgcacccca atgatctggt ccggtgcgcc
    16201 gcgcgactcc ggcggaggca gatgagcccg cagggcgatg agaaagggat tttgtgagtc
    16261 gtccagatct gtaccgacga cagattgccg atcctcgccc agcactgcga gcatcggtcc
    16321 attgaccctc aatattctgt tctcatcatc gagaacgacg accatatctg aggaaaagtc
    16381 aagcatcgca gagatgggaa cgcgatggga gaggaagtat accttcgcag tccccatctc
    16441 ctgcatatcg atctgcccgg agatgagaag catatcaaga tattttgcta cgaggttccg
    16501 gttcatgtgc attttagaag aaacgtccga gatggtcatg cccctcggat tccatttcag
    16561 gtgttgtttg atttgatcca tcctctcctg atcgagcacg ctcatacaag gtgatagggt
    16621 gctgggatag taaatttttt tatttggcag gccttgaaac gggtaaccgg gtttctggta
    16681 gctcccctct cttccggcac tgattcaaca tgctgtcatc cccgtggtaa gccgattatt
    16741 gcggttgctc tgggtgcacg attacgccta aaaataagta tataataatt tgcatggcag
    16801 atcctgttgc ataaggcaat tgcagaccaa tacggcgtta ttatcgtgca aattttcatt
    16861 ataaggccgt tagtaattta gttcagtcac aaagcaactg gcaatgcgcc aggtgaccac
    16921 gggagagaga agcgatgcag aaatatgatt tcaccggcgc ctcgaacggg gcagattgcc
    16981 tggttcggca ggcacgggaa aagtcgatgc atggagatca tactgctgca gttgaatatc
    17041 tgatggaggc agtcgatatc gagcccaaac acccggaggc gtttgttctg atgggagatt
    17101 gctgcgacta cctcggtcag tatgaacagg caatcgcgaa ctatgatcgg gcactcgagc
    17161 tcgatcccta tcatgctgat gcatggttca acaagggaat gagcttgaga tcgatcggga
    17221 ggaacataga agcagtgcag tgcattgaga aatcaatcga actgtattgc ggccgctgag
    17281 gcagcagcaa gttaccacac agcacaactt tcgacaacac cacaacaaag ggtccggaaa
    17341 gcaatcgagg gagaagggat gcatccctct tttgaggtta tgttaaaggg cagggtaact
    17401 gatgaacaat tccaccggca tatcgccgct ctgtgctgcc ataggtattt ctgtgcctat
    17461 ctgttcggat caatgcaccg tcataccacc ggttcggtta aggagcagaa ctgccgtact
    17521 ttgaacaatt ggttatggaa tatctgatgt gctctcttgc cctcataccc tttgccgttc
    17581 caggttccgg ggtgatcttt gcgcgatgaa cgttcacttc ctcgccgaga accgttggta
    17641 tctcaacaca aattatttaa ctgttgatca cttatggggg taataagcac atggcggtta
    17701 ccgggcagga acttttgatg gttggtgata tggaaagcag gaaacaggta ccgcacaggt
    17761 gacaccgttc gtgagtctgc gggtctggtg atgcggtgcg atgattttcc ggagataccg
    17821 gcgattcggg ttgaacattt caaaaataga gcctgttttt cccgctcgtc agggaaaaga
    17881 tcggccttca tggagtgaaa tgcgggaaac gactgtaatt cacacgacgg gagaatgtga
    17941 ggtcttaact tgaaagttac tgaagatgga gaacaagccg atgatagtgc tctatctatc
    18001 gttctggtat ttttcgatgt ctatcagaaa tccatagagg ttacgatcag aaggaccatg
    18061 gaagaatttc tctcatcact tcagagtgaa ctttcgagac ctttcgatct ttcaacgatg
    18121 catcagcaca atccgttaat atcccagttc aagcataaga aagaggggtt ctgcccgatc
    18181 ttgcacgaga tcgatggttc catcaagata ggcatcacag agatcggcat ctgggatccg
    18241 gtgccgcctt cacgattcct gtatggtgtt gagtgcgatg cccatgcctc tcttctgtcc
    18301 ctctaccggt tcaggcagga aagcacagaa acagatcagt tgctgcaacg cctgaggaaa
    18361 gcaactatca agatctttgg ggtggcgtgc ggcctttatc attgtgatga tcccggctgt
    18421 tttctgacat accacatgac gatatcggac cttgacactg gtatgtacgc ctgcagaggg
    18481 tgtagagatg attttatccg cgcattgcag tccctgaata gccggatagg ggcgtagaat
    18541 tactccacgt cctcttctca ggacgaagcg tgcggctttc accgcactcc gcccgggcgc
    18601 ttcacaacca gaatttcggg cggcagtatg cgaagagtga tgatcgtact ccagggtgtc
    18661 tgttatctca taccctcctt tctggcgggg aggtatcttc cgtccagata gggatctgca
    18721 tcgagtttca ccagggagcg cctgcaaatc ttcgtaccgg caaataggat gatcttaagg
    18781 agagtaaaga ggataatccc ggtgaactgc cccgcgctct cgggcgggtc ttcccgctcc
    18841 gcccccgtca cccctgcacg gtaaacaact caacacttca acacattcaa gtctgatgct
    18901 caacataagt agttgaatac tatggaatcc gactcatcac tcgagcacat cagaaggata
    18961 caggcgctga taggtcgcat tgaggagttg gctgaccgaa tcgatcgata ccagcaggga
    19021 agcaatgtcc agctcaacgc acttcgcgag gagtgcgatg ctgttcttgc cgaagcaaaa
    19081 caggactatc acgccttgat cgtatcagcg gagatgaggg ggcaggatgt aacccccgat
    19141 attgcccttg aggatcggcg attgaagaac acctggcttg ccctttaccg gcaccacggg
    19201 ggagcaaccg cagaagagat ctccggcgag ttgaagcgcc atagaacgac cgtctcaaca
    19261 tacctgaaca cacttgtcct gatgggctac gcgaaaaaag aacgggtagg gcacgaggtt
    19321 cattatactg caatcctgaa taaggattga ggaactactt cgcactctcg ggcacggctt
    19381 cccggctatc atcgaacccg ccccgaagag gacttacacg atctccacag gcgtgaataa
    19441 ctctgttcgg gcaggaatcg ccctgcagca gagtgtaaca gtccggtcga ttgatcgtcg
    19501 caacgcagat accccctgtt tggagaaaaa cgcacaatat gggaagggcg gcggtgcttt
    19561 catccccgcg ctctcgaccg gggtcttccc gctctgcccc ataagataaa ccgatttgca
    19621 agagcggcat tcttcactta cccataaccc ggaaaatacc atgcgagggg tgagattcga
    19681 actcacgaac tcctacgaga ataggccctc aacctatcgc ctttgacctg gcttggcaac
    19741 cctcgcacgc attcgggaat cgccggtcct gcaccgggca atccccgtcc tgctctttat
    19801 tatagcacac tgcaaccgga aataggttgc tctccgggcg cccggggcat acccttccgc
    19861 cggtgcccca ctcccgcctt cggtgtatat ttatgcgccc ggttccaaca ctcccgcatg
    19921 tgggccgata tatcacggga gtttgccgat tcaccgtccc agaccagggt ggtccggttc
    19981 ctgctcgaga acgggttcgg tatcaacgaa gaggggcgca tcgtctgcaa cggcgtcgag
    20041 atccccgcca cgcacatcgg cagggcgatc gagaccgaca ggagggtcgt ggacgccacg
    20101 gcccggcgca tcctcgataa cccggatctc cgggaggtct tcatccgtat gcgcgcctcg
    20161 cccgacctct cccgggttgc agaagccctc ggcctttcgg tcatcacgct ctacccgaaa
    20221 gacgcgcagc agaaggggat cgtcggtgcg gcggtcaagg tcctcgtcga ccacgacctc
    20281 tccatccgcc agatcttcgt caccgacccc tacctcgcgg aggaaccaag actcgtgatg
    20341 atcgtcgacg ggcgccgggt cccggcatcg gtctacgaag acctgcgcgc gctcccgcag
    20401 gtaaaacggt tgatcatctg aaaagttcca tttccagccc gagccggcgg atccgcttcc
    20461 tctgccggcg cgggagggcg atcgcgtgca tcatccgctc ctccatcgtt atggcgcgga
    20521 tccccttcac caggttgtcg gcatgggcga cgatcttctc ttcaagcgtt gcgggcatgc
    20581 agtcgcgcgg tgcaaggcag aggagcgagc actcgtcggc cgtcagcccg gcaccgatgt
    20641 gccgctcgac gattctctcg atcctctcgt cgaggccgaa cgcgcggcat atcgcgccgc
    20701 ccgcctcggc gtgatggagg tcgtgcgtca ctccccggcc gatgtcgtgg aggagggcgc
    20761 cggcctcgat gaggtcgcgg tcggcgatac cgtcggcata ggtgagcgca aggtcgcgca
    20821 ccgcacggca atgcgcgacg acgtcgtcgg agcacccggc gcgcccgagg agggcgatgc
    20881 agtcctcttc acgcactccc gaggctctcc ttgatccggt cgaccctccg ccggagggcg
    20941 tttgcgatga gttcgttgtc gaagtgctca agcatctcct cgcagttcgg acactcgaag
    21001 ttccagcccg ccgcatccgt gaacgtgtag atgatgccgc agtctttgca catgtaaaag
    21061 tcgttcttct cctcgtaggt gagccgtgca tcgaggtgtt cgagcacgtc gcggatctcc
    21121 tcctcgatca cgccgtggag gcggtccagg cggaggtgcc agaggtaggt gagccagccg
    21181 gtctcggtgt tcttcaggcg ccggtactcg gcgagacgct tctcgtagag ggtgtagagg
    21241 gtgtggcgga cggtgttgag gttgaccccg gtcttctcgg cgagttcctc gtcgctgtgc
    21301 tccccgccct cagggaaccg cttgagaagt tcgacgccct cctccccgat cagccgtaga
    21361 acataagcgt tgattgccgg atcatttaag agttcagtga tagaaaccat atttcattct
    21421 cccccagaga tgatattcta taataggcgc tcgggatcga tacctatcgt attgtacact
    21481 caacccttat cttcttttcc tattctcccg aacggctcac cccccgatca gcccccggac
    21541 ggccgcaata tttcgctcga gatcgtgaaa tgccgccgcc tccgtctccg cgcacccgaa
    21601 tatgctcacg acggcctgac cctcctcaaa ctccgttccc ggccagggaa tgtcggcgac
    21661 gcgcggcgcg agcgccgaga ggtccgcatc gagccgcatg tcgcgctccg caaagaggat
    21721 ccgccggacc gcgacccttc gcgccggagg cctcgttgcc gggagaaccc cgcggcaggc
    21781 gtccatgtgc agggcaaaga cgttctcccc cgtcgccatc tcgaccgtat ccagggtggc
    21841 ctggaaccgg gggttgatct cgatcgccca gggtttctcc ccggccatga agtcgatgcc
    21901 gaccgaacct gtgcacccgc tggcggcggc tatccgctcc gcgcacgcga ccatctcccc
    21961 ggcaagcggg tgggagaacg gggtgaccga cccggcgaac ccgtgcgcgc actcgccctc
    22021 gccccggagg atctgccggt tgaccgccac ggccagcgcg cgccggccgt ccgcgatgca
    22081 ggagacgctc gagggtatcc cgtcgacgag ttgctgtgca acatacggca catccggcca
    22141 ggcctcttcc catcggcgga ggtcttcctc cgcccacgcg accgcgtttc gccatcctcc
    22201 cgctccccgg cgcggtttga ccatcgccgg gtagcgatcg cgccccgcaa gaggcgggac
    22261 cggaacgtcc agcccctcga agaaccgctg gatctcgagt ttgtcgagga accgctccac
    22321 cttcgccggc ggcgtcccgc agagcggtat cgtcgtcccg acggcctcgg cgcccgatgc
    22381 gacgacgagc gcgtcgaccg ggtggcgggc ggcaagttcc gctatctttg ccgggaggtc
    22441 ggcgagttcg tcgaacgaga ggcggtcctc cgtgtaccag gcgaggtccc ggtcgcagaa
    22501 atggtcgacg gcgtagaccg tgtagccggc ccggcaggcc gactgcgcta tgtgccgcgt
    22561 ggcaaacccg gcaaccagca cccgcccctt caccgctcca ccgtcttttt gcctgctttc
    22621 gccggttcga tccggatctt cgcatccggg tactcccggt tgagctcccg cccctcgaag
    22681 aggtggtcga ggaagagggc gaggctcgat atctccgagt ggggctgggt cgtcaccgag
    22741 acgttgtagt cggcaagacc gtatatgtcg cccgggacct tctccgcccc cacaacgacg
    22801 agcacccgtt cttcgcgccg gatgtcgtcg atgacgtcgg tcatgcggag cccgtacatc
    22861 gtgaggtggg cgaccttgcc gccgccggcc ttccagtccc ctatgcaccg gcgccacgag
    22921 acgccgttct ccacgaagaa gtctcctccc caccgggagg ccacgtccgt tatgctcgtt
    22981 atgacaccgg ggtcgtccgc cgcgagatac atgccccggg cgccgagcgc ccgcgccgcg
    23041 agtccgacgt gggtcgtcac ccgctgatcg cgttcgggcc ggtggcctac ccggagcacc
    23101 gctacggtgg gcgcgccgct caatcccctc tcctcgttgc cctgacgacg ccgttctcga
    23161 tgacgaacgg gagatcatcc ttcgtatggc ggcactggca cgcgatcagc gactcctgga
    23221 gagagagaag ccgtacccca tcgccggacc gccggatcag gagatagtgc tcgaggcgct
    23281 caagcgatgc catgaccgca ccgggttcaa acccggtggc tgctaccagg tccggggtcg
    23341 taatgtcgct gttttcggga atacagtggt atacggtcca gtccagatct tcctcacgca
    23401 cacctattct tttttccggg cggaccgata tacttattca tgcgccgccc gcaccgttcc
    23461 ggccgggagg gccgctgcca gggcggttct ccgggaggaa aggggcgcgg ggcagggtga
    23521 aacgacgtat ggccgattat atggatcttg cggatatcgc cgagaggctc tttgaagcga
    23581 gcgacgacga tgacgaacgc ctcgcgggga ggctcgatac gctcgacgaa gagacccggc
    23641 gagcgctgct tgcctccgac ctcctcaacg cctaccaggt cttttacttc tggttccggg
    23701 agacgcccga cgaccttgcg atggagcgg
//
LOCUS       DAOSEE010000016        22969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_993_length_22969_cov_235.336912, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000016 DAOSEE010000000
VERSION     DAOSEE010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 22969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22969
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_993_length_22969_cov_235.336912"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="PLI76_02310"
     CDS             complement(<1..996)
                     /locus_tag="PLI76_02310"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HOI60894.1"
                     /translation="MAEREAGQVLATRPEPGERIDLMRARDVADIKVQNPEGENIGDI
                     TDIAIDRDGGCIAYAVLAYGGILGFGEKHFAIPWEAVRVRSREKIAIVDTDKKTLDNA
                     SGFTRDKMPREGDWSVIRTPPRPTAAKERPAAEGERPPPKEEKPAAPAEPAPRTVESY
                     TPVPPPVQTVAGGWGEKAMKPRTEEQPARAAGAVVEEVRREEPARAEAKATEVRHEEP
                     ARTETETVEVRRETPAGAEREQPVTTPGPTPGHVSAADLQVYLRGIDYPAGRQDLIAH
                     AQKNNAPEGVIAVLEGFGDRTYRSAAEVSEEFGREARHETPAGAETVVEERPETRA"
     gene            complement(1123..2436)
                     /locus_tag="PLI76_02315"
     CDS             complement(1123..2436)
                     /locus_tag="PLI76_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-coenzyme M reductase glutamine
                     C-methyltransferase"
                     /protein_id="HOI60895.1"
                     /translation="MDIAIFSPGIYTYGAMLIGGVLRDAGHRVSLTRAPAVPADSLLL
                     ASLFSTQHLLDPAIRDLVRTHREKGGKVYVGGPVSAAPEIVLGELAPDAVVVGEGEET
                     VVRLVAEGVSAALPGIAYLDGGRPVVNPPAPPASIDRPLPLIPGDIGSQSIRGASAYI
                     ETHRGCIGGCTFCQVPRFFGRKIRSRELASIIEEVRAFSAAGATRLSVSGGTGSLYGS
                     ADGEMNPEAFIALLHGMAEVMGARNVSSPDIRVDCITDEILDAVRRYTIGWVFFGIES
                     GSARVLRLMGKGATVRQVEEAVGRCREHGLRVAGSFIVGYPGETERDYEATKDLIARL
                     ALDDVFVSGAEPIPGTPLADLAVRTPRDKNPAFVPHTGEYRPLNLTESEARCFDLMMH
                     ADMFRPQMRLVTDEVYATYLGEARKQGQDIRAATELILRYSPSGA"
     gene            complement(2494..3171)
                     /locus_tag="PLI76_02320"
     CDS             complement(2494..3171)
                     /locus_tag="PLI76_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60896.1"
                     /translation="MTRLRYLTTTCAVCGESCRFTIPPGAVSAGSRDLDTRPAEPLRS
                     TIYAWVKRCPSCGYCAPDPGKALAGAADAVRLPRYRWQLDARGFPDIANTFLCWSIIQ
                     EDLGAPAQAAWASLHAAWVCDDEGEDVPARVCRRRAADLLQRAREQDERLAPLEGADE
                     ALLVDLLRRAGRFREARAVAARVLDQDPQPLIAGIVRLQVRLIAASDRAAHTVAEARR
                     SRQPAPL"
     gene            complement(3267..4352)
                     /locus_tag="PLI76_02325"
     CDS             complement(3267..4352)
                     /locus_tag="PLI76_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI60897.1"
                     /translation="MNARHIGIVAKKELLGLSAEKTIVFAILLQVFIAMFSSFLMVGL
                     TAMYDPEALEGYSTATYGVGYAGADSALIDEFEKRDDLILHRMDLSPALAALRDRKIA
                     AVVYVPDTPPDAEGPVAITLYLVQNDLQSSVINVKIKEVLQGYERELREVRADRMTAF
                     PVGLNFPDAGDAESFYEFVYGLLIPLLVLLPAIVSAALIIDLITEEYQRQTLDTLLST
                     PITFTEMVAGKIAACFAIVPVQAGAWILLLELNGIAVANAVPILLHVSAAGLFLILLG
                     AAIALHYRERTSAQFVFSTGLVVVFLLAMAIPYNPLNIVVRLAVGTAGPVHWLILLLV
                     SGATALLAFAVAAYGRRVGKISPTAVR"
     gene            complement(4349..6154)
                     /locus_tag="PLI76_02330"
     CDS             complement(4349..6154)
                     /locus_tag="PLI76_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HOI60898.1"
                     /translation="MSLASSIRTIAVWEMNRSMTTMGRGVLPLAAGLLVLLVLVTAFA
                     AESGIHMQDGIYRIGIDDPDVARIVDADTRFTVETVSGPALWNNRFAYDIVVIRGEVY
                     AADTGKGRAALTALKRDYEGYVSRIAAGEPDLFAGYPLWIDLEYVKSEIDFLATQSGQ
                     QIGAPANARAPSVPSGPVEAVTLPPSTVPVHEEDLRQHLAGMEKTNPLSRYTGIVSSG
                     SATDGLRTPSELSPPLPYDALILVFVFIFPLYFTSQFFMMSVMNERVGRAGEALLAAP
                     VRPAAIIVGKAIPYFAIMLLVSAAITFFIGAPLTILLPLIPVILFFLSSALIIGMAAR
                     SFKELSFVSIFFSTLATSYLFFPTVFANTHVVSLISPLTLVILELQGEGFTAAGYVYS
                     TALFFATSAVLFYAGTINFREERLFSEKSLTSRLMDFVAGAISRDRPHFSLFLLAAFT
                     IPFVFMAQMMLLVLFFNIPMPLSLVLLTVSAAFIEEVAKSVGLYAVAGERPGFLTPKN
                     LLVGAAAIGFGFLAGEKLLLFATLSQITESIFGSVLFLSLQVLWMPLLLHIAGVLITG
                     TFLLAGGKRAYVPGLVVASVVHSLYNLHFLLGGLV"
     gene            6234..7121
                     /locus_tag="PLI76_02335"
     CDS             6234..7121
                     /locus_tag="PLI76_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI60899.1"
                     /translation="MITARSLAKHYGDFPALDDVTFDLDDARILGVIGHNGAGKTTLL
                     KIMAGLVTPTAGNLLIDGIDVVRRPFNLKRNLGYLPEESRLYETMTTDTYLAFFGEIY
                     GMPKAAIRTRRDDLLAGLALEPDGKKIGELSKGMKRKVAIARSLMHDPGLLIYDEPTS
                     GLDPMTSRSVLEYIKGLRDRGKTVIFSAHNLSQVEEVCDRVLILRRGRVVAGGTIAEL
                     RETFGSITYRVFFRIPDLSSFAPPVEYSAHDGRYLAEARSIDELNRTTAALAAEGAGI
                     DRIESHYPTLEEMLLKIGR"
     gene            complement(7484..7556)
                     /locus_tag="PLI76_02340"
     tRNA            complement(7484..7556)
                     /locus_tag="PLI76_02340"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7521..7523),aa:Lys,seq:ctt)
     gene            7670..9133
                     /locus_tag="PLI76_02345"
     CDS             7670..9133
                     /locus_tag="PLI76_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOI60900.1"
                     /translation="MPEAVQNVSKERIKYIIFGCGSTGYNVAEELGQESEDLVIVDKD
                     EKRVEDLRDQKYEALVRDLRDPNLMEGLPVPEVAFILTNDRDANLTALKTIKNRYPAT
                     YVIARATDPVSVDLLQQEGADIVLYPQEVVARTAIHHIRKLHSSRLALRLYDLLAAWE
                     GTLCIVTHLNPDPDSISSAMALSMIARHASHNKLNCRILYEGDIGHQENRAFINLLEI
                     KMERLTPQILSECNYIALVDSSAPGANNELPKTTRVNIIVDHHKNGERPPGAADFVDI
                     RPGVGATASIMTQYLMELDIPVSKSVATALLYGIRADTRDFKRNVTPQDLNYAAFLLP
                     LTDSDLLDKITSPSISQETVEIMGNAIRNRRIRSGYLFSNVGFIRNRDALPQAADILI
                     HLEGVNTALVYGISDQNIVISGRNKDIRLHLGNVMAEAFGPIGEAGGHATMAAAMIPL
                     SYFSMVKEKEELLNLIIDPILKRFANIVGLDNNEGKQ"
     gene            9130..9753
                     /locus_tag="PLI76_02350"
     CDS             9130..9753
                     /locus_tag="PLI76_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HOI60901.1"
                     /translation="MRFADWEPHYTAILDYFGFERDADEAAARLLEETADGRDDVGLL
                     EALIRGRAVTVCGNAPSLPDELDRIEGTVLAADAAAEVLAGHGIRPDAVFTDLDGATD
                     VFVDLSRQGTVMVVHAHGDNMPLIRHWVPHLPGPLVATTQAAPFAHIHNFGGFTDGDR
                     AVFAADDLGAASIRIIGFDLADKNVDPVKRGKLYWAGELLRLIGYDL"
     gene            9743..11380
                     /locus_tag="PLI76_02355"
     CDS             9743..11380
                     /locus_tag="PLI76_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOI60902.1"
                     /translation="MTSNALIIDGYVDEPACLGVAPYISPYIREVAGVLVEHGYTTGY
                     VTIDQVRADPSLVAGHSDLVVMIAGLTVPGAYIGGKPATLTEIQQLGTLLREPATAIG
                     GPIAFGYAPGGGRKAIRQAIAGFDATLSGSPAVALDAWLSGGEPAGAGDYSLTDPWAV
                     AGAGIVARHPAFPYVMCELETATGCSRATVGGCSFCTEPFYGLPRYRSIEGIAREVAA
                     LHAAGARHFRLGRQPDLLAYQSHGGEFPAPRPEVLAELFSAVRESAPGLATLHIDNIN
                     PGTIAHHEDAAREALGAIVAGHTPGDTAAFGMETADPAVVAANNLKAMPDDVFRAIEV
                     ANDVGGKRTKGIPELLPGLNFVVGLAGETPATFDANEAFLRRVLDAGLLVRRVNIRQV
                     MPFEGTAAYEKNTLGAHDARFRAFKEWTRREFDEPMLRRVFPIGTVLRDVVVEIAGRP
                     SFGRQMGSYPILAGIPLDLPAGTVLDAVVVDWGMRSVTALPCPVEVNTLPVAALRWIP
                     GVGKKRAATIAARRPFENIAEFQKIVGETGIEGAMKF"
     gene            complement(11377..12513)
                     /locus_tag="PLI76_02360"
     CDS             complement(11377..12513)
                     /locus_tag="PLI76_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOI60903.1"
                     /translation="MNASLQIGKLAGIPVKLHWSFLLVIPLFAWIIGSQIRLTTELIA
                     TLFGVPIDVTLIAAGLNPYILGSIVALGLFVGVFIHEMAHSLIAKAKGIEIHSITLLI
                     LGGVSQMEETMPDPKIELPMALAGPMTSLAIGLISAALVYVFDAAVADPAVAGVLVFT
                     FGYLGLLNILLFGFNLLPAFPMDGGRVLRAWLARRMPLSRATRIAADVGKGFAVVFGI
                     VGLLFLNPILIIIAFFIYIGASQEATVLRYNILLQDITVADAMSSPVATVEPTLPLSR
                     LVEMMYETKHLGFPVVDRGALVGIVALADIHKITPQDREAMQVRDVMTRNPTTLPPSA
                     PLLDALKIITGKDIGRIPVVSDGALVGIVTRTDVLRVMELREED"
     gene            12567..12830
                     /locus_tag="PLI76_02365"
     CDS             12567..12830
                     /locus_tag="PLI76_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HOI60904.1"
                     /translation="MRTEITNLFELPVYTEKAIFVGNVDDIVVDVDQKKIDSLAVGNL
                     DPAIREVMKGSGLKIPFRIIKSIGNIVIIRHIPGLFRSPAKEE"
     gene            12858..13592
                     /locus_tag="PLI76_02370"
     CDS             12858..13592
                     /locus_tag="PLI76_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008193 - tRNA guanylyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(His) guanylyltransferase Thg1 family
                     protein"
                     /protein_id="HOI60905.1"
                     /translation="MDINDREIFSRLAIFPPFFVRLDGRAFHRLARALELTRPFDPRF
                     HAAMRSACRYLLTGSGLTPLFAYTFSDEISLYFSTLPFSGRVEKIDSVTAASAASALT
                     IELGCREPLAFDARVIPAAGEFAVEYLVSRQNEAWRNHINAYCQSALVDEGMSPRDAA
                     AALRGMRSEAMHEIMFERGVNLAATPAWQRRGTLLYREECIKEGYNPLTGERVEATRT
                     CIREPEETPLFSTVEGTAFIRSLIGA"
     gene            complement(13545..16754)
                     /gene="ileS"
                     /locus_tag="PLI76_02375"
     CDS             complement(13545..16754)
                     /gene="ileS"
                     /locus_tag="PLI76_02375"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOI60906.1"
                     /translation="MKEVTSSYNPRDIEAGVQDYWTKENTYARVRALREGGKAFFFVD
                     GPPYTTGHIHLGTAWNKIIKDTILRYHRMRGADVIERAGYDMHGLPIEVKVEHQLGFT
                     SKKDIEEYGIAAFVEQCRTFAVTHMGIMSDQFRRLGVWLDFDNPYQTIKEDYIESAWW
                     TLQRADERGLLERGHRVVNWCPRCETAIADSEVEYWDETDPSIFVKFPLSGRENEYLV
                     IWTTTPWTLPANVAVAVNPAFIYARVKAKKDDAEEILWIADELIEPVLKMGRYQDYTV
                     LERVSGSDLVGTEYTSPLAGCVPHQAEIRHRVVAADYVALENTGLVHIAPGHGWDDYL
                     IGIREGLEAFCPVDSGGCFTPAAGAFADMYVRDANEIVIDALGGYLLARRTITHRYGH
                     CWRCKTPIIYRATAQWFLKATVIRDSMLEEIAGVKWYPDWAGSARFHDFVKESRDWCI
                     SRQRYWGIPIPIWHCEHCGEYTVVGTIAELEERSGAKVPDPHRPYVDEVVIPCPCGGE
                     MRRVEDIFDVWFDSAVASWATLGFPREREAFDRLWPADFITEGQDQTRGWFYSQLGAS
                     NVAFARAPYKSVLMHGFALDAEGRKMSKSFGNVVTPEEVVNQFGVDVLRFYVLWANAP
                     WDDLKFNWDGVKTIHRTLNILWNVYRFPLPYMILDSFQPAAGEGERWDDSFVRSHIRD
                     MPEEDRWIVSRANTLIRAIGEDMQEYHLHRETRALAAFILEDLSRWYVQLVRPRMWLE
                     EDSPEKRYAYETFYYVMRRLITLLAPFTPYIVEEIYQNLRLAGDPESVHMLDWPEADD
                     LLIDPALEADMGVIQSFDDAVATARQAGKRKLRWPVSETVVVTGSDEVKTALQDLNDL
                     ARTRANARAVRVVTGTWERILWQAAPVMRAIGPEFGREGPKVKALIEGADGTALKAAI
                     EREGKAELDGYEITARHVAFTEALPAGVFAAPMKDATVYVDVALTPALEAEGYAREVI
                     RRIQEMRRQLDLNVDDYIVADVHVADGRIVALIGEEEWKDEIAGEVRAAVLTVRHADG
                     ERPAEPFALEKDWDVEGVQMRIGISRADKRPDKGRPLDR"
     gene            16870..17343
                     /locus_tag="PLI76_02380"
     CDS             16870..17343
                     /locus_tag="PLI76_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HOI60907.1"
                     /translation="MQIAVVGRGDCSGEEYAAARAVGRLIAGIGAVVCCGGLGGVMEA
                     ACRGAREAGGTTVGILPDTGDANPYVDVTIRTGMGHARNVVLVHSADTVIAVGGGYGT
                     LSEIAVALKTGKPVYGLATWEIEGVVACATPEEAVSRAGRPSRPSRSPRGPGEPA"
     gene            complement(17279..19132)
                     /locus_tag="PLI76_02385"
     CDS             complement(17279..19132)
                     /locus_tag="PLI76_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HOI60908.1"
                     /translation="MVKRYLSVISLAEARALVERSFPAIPGVVRVPVTAGTVGRITAA
                     PIFARFSVPAIHLSAMDGIAVRSAETLGASEQNPVTLPDAARVNTGNIVPAGYDAVVM
                     IEDVWVDGETYTVRKPVSPWQHIRPVGEDIGESEMILPSHHRIRPQDVGALANYGVTE
                     VAVENVRAGLIPTGSELVPAGEMPPPGKVVESNTLMAAAVLTEAGVEAHRYPIVPDDY
                     DLIREAVRRGVAENDILLVSAGSSAGTRDYTAEIVRDLGEVLAHGVAIKPGKPVIIGR
                     IDGKPVIGLPGYPLAAATVLREIVLPLVARYGLPTREGERVEARLTTTLQSDIGTDEF
                     VLLSVGNIGDRWVAVPQSRGAGIQMSAVRANGYLRIPEDMEGAEAGKTVDVRLTVPRA
                     EAEEAVLVTGSHDPALDYLADLVRPRGVDIHSTHLGSMGGVIALKKEECHAAPMHLLA
                     PDGTYNTYYLERYMPGADLALLCVAERQQGVISRDGLSFDDLPGRTFINRQKGSGTRM
                     LLDHTLAARGIDPASIPGYGREATTHLAVALAVRTGEADAGMGVYSAAKALDLAFVPV
                     ATERYELAMHRAMLDDPGIAALVETVSSAAFKQVLRDLGGYETDETGVLRG"
     gene            complement(19126..20328)
                     /locus_tag="PLI76_02390"
     CDS             complement(19126..20328)
                     /locus_tag="PLI76_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HOI60909.1"
                     /translation="MSLFLSVVSVGKAVEVVRGIAPPLESGDVPLENALHRVLAEDIR
                     ADIDIPGFDRSTVDGYAVIATDTTGASEAIPAMLSCRGRVGMGSADAGSITPGSCRYV
                     PTGGALPRGADAAVMIEHAEQIGDDVLVHRSVAPGENVITRGEDFRAGTVVLERGRVL
                     SPRDIGVAAAIGADRVTVAAVPRVGIISTGNEVVPVTATPGPGEVRDANSYLAGAFVT
                     ERGCRPVYFGIVRDEREALKAAVSSALDTCDVVLVSGGSSKDERDNTAAVVADLGEVL
                     VHGVALAPGKPTIIGTARGRPVIGLPGHPASTFVVLVAIVDHLLAAMRGTAVSRRTLS
                     ARLKQNIPSTRGREDYVRVSVRDGEAVPIFGKSGLLNTLVQSDGIVRVPASSEGLEVG
                     EEVEVILW"
     gene            complement(20325..20507)
                     /locus_tag="PLI76_02395"
     CDS             complement(20325..20507)
                     /locus_tag="PLI76_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60910.1"
                     /translation="MDEEKVRKAFEDYGITDEITCPQAFDISEKYNIPKMDIARYCNQ
                     REPRIKIRSCQLGCFR"
     gene            20600..21913
                     /locus_tag="PLI76_02400"
     CDS             20600..21913
                     /locus_tag="PLI76_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048063716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOI60911.1"
                     /translation="MRIALINSRVDAAGVNIRDHLGVLLTEGGRWPLADDHELVFHEV
                     AGRLIYQDRIDEEVDADLIVFISRHASAQPVPALTVHVTGNYEAADLGGEPGALAPAA
                     PAWMHAILGNLAARAPEGYRVSYEVTHHGPTALATPSLFVEIGSTAAEWADPAAGRAV
                     AESILAAAPQETIDLIGFGGTHYAVRQTAIALASRGAFGHIAPTRQIGALDSGRIRQM
                     REASRAVAAYIDKKSLSADEAARVERLLDGAGIPLLTESEIREIGDLEWATYLRIRAL
                     ADEIAPGSRARIHGLAGQGTPVPVDVNRDLVEETAKSDKTGFITALDGLPVAHLSKGS
                     TEVLPTFIGFVYGSSRLASDITTLCVKLLLISEDAVIDGDHLVLRKVRFDPEKARRLG
                     VPRGPLFAMLAGGKAVEIGGQTVTPDAVQATSIKRIHIPGLERYI"
     gene            21910..>22969
                     /gene="ftsZ"
                     /locus_tag="PLI76_02405"
     CDS             21910..>22969
                     /gene="ftsZ"
                     /locus_tag="PLI76_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOI60912.1"
                     /translation="MKSIVEEALSRAREEQRFAEPTGVDKDIEDVLMELRTEIAVVGC
                     GGGGSNTITRMSEEGINGARLIALNTDAQHLIRTHAETRILIGRQRTRGLGAGSIPQV
                     GEEAALENEDDIKRAVEGCDMVFITTGLGGGTGTGSAPVVAKAAREEGALTIAVVTLP
                     FTAEGAIRAQNAEAGLERLREVADTVIVVPNDRLLEVVPRLPLHAAFKVSDEVLMRAV
                     KGITELITMPGLVNLDFADVRTVMERGGVAMIGMGESDSEDKAVDSVKKALRSPLLDV
                     DISGATAALVNVVGGPDMTMYEAEGVIQEVYDRIDPDARIIWGAQVDPDMQGRMRTLL
                     VITGVRSPQIYGRNEKSMP"
BASE COUNT         3994 a         7496 c         7560 g         3919 t
ORIGIN      
        1 cgcccgggtc tcgggccgct cctcgacgac ggtctccgcc ccggccggcg tctcgtgccg
       61 ggcttccctg ccgaactcct cgctcacctc ggcagcggaa cggtaggtcc ggtcgccaaa
      121 cccctcgagc accgcgatca cgccctccgg ggcgttgttc ttctgcgcat gcgcgatgag
      181 gtcctgcctt cccgccgggt aatcgatacc cctgaggtag acctgcaggt cggctgcgga
      241 gacgtgcccc ggtgtcgggc ccggggtcgt gacgggttgt tctctctccg ccccggccgg
      301 cgtctcgcgc ctgacctcca cggtctcggt ctccgtccgg gccggttcct cgtgccggac
      361 ctccgtggcc tttgcctccg cccgtgccgg ctcttcacgc cggacctcct ccacgacggc
      421 ccccgcggcc cgggccggct gctcctcggt tcgtggtttc atcgccttct ctccccaacc
      481 gcccgcgacc gtctggacgg gcggcggcac cggggtgtaa ctctctacag tgcggggtgc
      541 gggctctgcc ggtgctgccg gcttctcctc tttcggcggc gggcgctctc cttctgcagc
      601 aggacgctct tttgccgcag tcgggcgcgg gggtgtcctg atcacgctcc agtcgccttc
      661 ccgtggcatt ttgtcacggg tgaaaccgga agcgttatcg agcgtcttct tatccgtatc
      721 cacgatagcg atcttttcgc gggagcggac ccggactgcc tcccagggta tggcaaagtg
      781 cttctcgcca aacccgagta taccgccgta cgcaagcact gcgtacgcga tgcagccacc
      841 atcgcggtcg atagcgatat cggtgatgtc gccgatgttc tccccttccg ggttctgtac
      901 cttgatgtcc gccacgtcgc gtgcgcgcat caggtctatc ctctctccag gctccggccg
      961 ggttgccagt acctgtcctg cctctctctc tgccaatcgt ctcgcctcca acctgtttac
     1021 ctgcgaaggg atatcccctt caggacgggg gagtagagga tcgagtatac ttaagggttt
     1081 cccgccccga aaaaccggga gatgaggctg ccggcgcggc gcttatgcgc cggacggcga
     1141 gtagcggagg ataagttcgg ttgccgccct gatatcctgc ccctgcttcc ttgcctcgcc
     1201 aaggtaggtg gcgtagacct cgtcggtcac gagccgcatc tgcgggcgga acatatcggc
     1261 atgcatcatc aggtcgaagc atcgtgcttc gctctcggtg aggttgagcg gtcggtactc
     1321 accggtgtgc ggcacgaacg ccgggttctt atcccggggc gtcctgaccg cgaggtcggc
     1381 aagcggcgtc cccgggatag gctcggcgcc gctcacgaag acgtcgtcga gggcgaggcg
     1441 ggcgatcagg tcttttgtcg cctcgtagtc ccgctccgtt tcccccgggt agccgacgat
     1501 gaaacttccc gcgacccgga ggccgtgctc ccggcaccgc cccaccgcct cctcgacctg
     1561 ccggacggtt gctcccttgc ccatcaaccg gagcacccgg gcactccccg actcgatacc
     1621 aaaaaagacc caccctatcg tgtatcgccg gacggcgtcc aggatctcgt cggtgatgca
     1681 gtccacgcgg atgtcggggg aggagacgtt tcgcgctccc atcacctccg ccatgccgtg
     1741 gaggagggcg atgaacgcct ccgggttcat ctcgccgtcg gccgacccat agagcgaccc
     1801 ggtccccccg gagaccgaga gcctcgtcgc cccggctgcc gagaacgccc tcacctcctc
     1861 gatgatgctc gcgagttcgc ggctccggat cttcctcccg aagaaccggg gcacctggca
     1921 gaaggtgcat cccccgatac acccccggtg ggtctcgatg taggcgctcg cgccccggat
     1981 gctctggctc ccgatatcgc ccggtatgag ggggagcggc cggtcgatcg aggccggcgg
     2041 ggcgggaggg ttcacgaccg gtcggccgcc gtcaagatag gcgatgccgg ggagggcggc
     2101 cgacaccccc tccgcgacga ggcggaccac cgtctcctcg ccctccccga ccaccacggc
     2161 gtcgggggcg agctctccga gaactatctc cggcgcggcc gagaccgggc ccccgacgta
     2221 gaccttccct cccttctcgc ggtgggtccg gacgaggtcc cggatggcgg ggtcgaggag
     2281 gtgctgggtc gaaaaaaggc ttgcgaggag gagggagtcc gccgggacgg cgggcgcccg
     2341 ggtgagggag acccggtgcc ctgcgtcacg gaggacgccg ccgatcagca tggcgccgta
     2401 ggtgtagatg ccgggggaga agatcgcgat atccatgatg tacagtattg taagaacagt
     2461 atacaaatgg tggggctctg ccgaattctc cggtcagagc ggtgccggct gcctggaccg
     2521 ccgcgcctcg gcgacggtgt gggccgcccg gtccgacgca gcgatcagcc ggacctggag
     2581 ccgcacgata ccggcgatga gaggctgcgg atcctgatcc agcacccgcg ccgccacggc
     2641 gcgggcctcc ctgaaccggc cggcccggcg gagcaggtcc acgaggagcg cctcgtcggc
     2701 cccttccagg ggggccaggc gttcgtcctg ctcccgggcc cgctgcagga gatcggcggc
     2761 gcgcctccgg cagacccggg ccggcacatc ctccccctcg tcgtcgcaga cccaggcggc
     2821 atggagggac gcccacgccg cctgggccgg ggcgccgagg tcctcctgta tgatcgacca
     2881 gcagaggaag gtgttggcga tatccggaaa cccgcgggcg tcaagctgcc agcggtagcg
     2941 cgggagtctc accgcatcgg ccgcccccgc gagggctttg cccgggtcgg gggcgcagta
     3001 gccgcaggaa gggcatcgtt tcacccacgc gtatatggtg gaccggaggg gttcggcagg
     3061 gcgggtgtcg aggtcccgcg acccggcgct cacggcgccc gggggaatcg tgaaacggca
     3121 ggactcgccg cagactgcac aggtggtggt gaggtagcga aggcgggtca tcgatgggct
     3181 gaattgcata acctaaaata tataattttc ccctgatgga atgcggcgag agagcggacc
     3241 caccagaaga ctccccgccg aacccatcac cggaccgctg tcggggagat cttcccgacc
     3301 cgccggccgt aggccgcgac ggcgaaggcg aggagcgccg tcgcgccact gaccaggagg
     3361 aggatcagcc agtgaaccgg gccggcggtc cccaccgcca gcctgaccac gatgttgagc
     3421 gggttatagg ggatggccat ggcgaggagg aagaccacga cgagccccgt cgagaagacg
     3481 aactgggcgc tggtccgctc gcggtagtgg agcgcaatcg cggcaccgag caggatgagg
     3541 aagagcccgg ccgccgagac gtggaggagg atcgggacgg cgtttgctac tgcaataccg
     3601 ttcagctcga gaaggagtat ccaggccccg gcctggaccg ggacgatggc aaagcaggcg
     3661 gcaatctttc cggcgaccat ctccgtgaac gtgatcggcg tcgagagaag ggtgtcgagg
     3721 gtctggcgct ggtactcctc ggtgatgagg tcgatgatca gggccgccga tacgatcgcc
     3781 gggaggagga cgagcagcgg gatgaggagg ccgtagacga actcgtagaa actctccgca
     3841 tcgccggcgt cggggaagtt cagcccgacg gggaaggcgg tcatccggtc ggcccgcacc
     3901 tccctgagtt ccctctcgta gccctggagg acctccttga tcttcacgtt gatgacgctc
     3961 gactgcaggt cgttctggac gaggtagagg gttatcgcga ccggtccttc ggcgtcgggg
     4021 ggcgtatcgg ggacatagac caccgccgcg atcttccggt cgcgcagcgc tgcaagcgcc
     4081 ggggagaggt ccatccggtg caggatgagg tcgtcgcgct tctcgaactc gtcgatgagg
     4141 gccgagtccg ccccggcgta accgacgccg taggtggccg tcgagtagcc ctcgagcgcc
     4201 tccgggtcgt acatggcggt gagccccacc atcaggaacg aggagaacat ggcgatgaag
     4261 acctgcagga ggatggcaaa gacgatcgtc ttctctgcag agaggccgag gagctccttc
     4321 tttgcgacga taccgatgtg gcgggcgttc ataccagccc tccgagaagg aagtggaggt
     4381 tatagaggct gtggaccacg cttgccacga cgaggccggg cacgtaggcg cgcttcccgc
     4441 cggcgagcag gaaggttccg gtgatcagga cgcccgcgat atggaggagg agcggcatcc
     4501 agagcacctg gagggagagg aagaggacgc tcccgaagat cgactcggtg atctgagaga
     4561 gcgtggcaaa gaggaggagc ttctcgccgg caaggaaccc gaagcctatg gcggccgctc
     4621 ccacgagcag gttctttggc gtgaggaacc ccggccgctc gcctgctacc gcgtagagtc
     4681 cgacggactt cgcgacctcc tcgatgaacg ccgcagagac cgtcaggagg acgagcgaga
     4741 gcggcatcgg gatgttgaag aagaggacga gcagcatcat ctgggccatg aagacgaacg
     4801 gtatcgtgaa ggcggcaagg aggaagagcg agaagtgcgg gcggtcgcgg ctgatcgcgc
     4861 ccgcgacgaa gtccatgagc cttgatgtga gggatttctc cgagaagagg cgctcttcgc
     4921 ggaagttgat cgtcccggcg tagaagagga cggcgctcgt ggcaaagaag agcgcggtcg
     4981 agtagacgta tcccgcggcg gtgaaccctt cgccctggag ttcgaggatg accagggtga
     5041 gcggggatat gaggctcacg acgtgggtgt ttgcaaagac cgtcgggaag aagaggtagg
     5101 acgtggcaag cgtcgagaag aagatcgaga cgaacgagag ttctttgaag ctccttgcgg
     5161 ccatccctat gatcagggcg cttgagagga agaagaggat gaccggtatg agcgggagga
     5221 gtatcgtgag cggtgcgccg atgaagaagg tgatcgcggc cgagacgagg agcatgatcg
     5281 cgaagtacgg gatcgccttc ccgacgatga tcgccgccgg ccggacgggg gcggcaagga
     5341 gcgcctcccc ggcccttccc acccgctcgt tcatcacgct catcatgaag aactgggagg
     5401 tgaagtagag ggggaagatg aagacgaaga ccaggatgag cgcatcgtac gggagcgggg
     5461 gcgagagttc cgacggcgtc cggagtccgt ctgttgccga accgctcgat acgatgccgg
     5521 tgtagcggga gaggggattt gttttctcca taccggcaag gtgctgccgg aggtcctcct
     5581 cgtggaccgg gaccgtcgac ggcgggaggg tcaccgcctc caccgggccg gacgggacgg
     5641 agggtgcccg ggcatttgcc ggagccccga tctgctgtcc gctctgggtc gccaggaagt
     5701 cgatctcgct cttgacgtac tccagatcga tccagagcgg gtagccggcg aagaggtcgg
     5761 gttctcctgc ggcaatccgg gagacgtaac cctcgtaatc gcgtttgagc gcggtgaggg
     5821 ccgcccgccc tttgccggta tcggcggcgt agacctcccc gcgaatgacg acgatatcgt
     5881 acgcgaaccg gttgttccag agagcgggtc cgctgaccgt ctcgacggtg aagcgggtgt
     5941 cggcgtcgac tatccgggcg acatcggggt catcgatgcc gatccggtag atgccgtcct
     6001 gcatatggat gccgctctct gcggcgaacg cggtcaccag gaccaggagg acgagcagtc
     6061 ccgcagcgag cggcaggacg cctcttccca tcgtggtcat cgaccggttc atctcccaga
     6121 cggcgatcgt ccggatggag gaagcaagac tcatggttat ccccggtagt ctcagaggag
     6181 catacctctg tggagcaacc tattagtagt catcggagcc ataggaagat tcgatgatca
     6241 ccgcccgctc tcttgccaag cactacggcg acttcccggc cctcgacgac gtcacgttcg
     6301 atctcgacga cgcacggata ctcggtgtga tcgggcataa cggggccggc aagacgacgc
     6361 tcttgaagat catggccggc ctcgtcacgc cgacggcggg gaatcttctg atcgacggca
     6421 tcgatgtcgt gagaaggccg tttaacctga aacggaacct gggctacctt cccgaggagt
     6481 cacggctcta cgagacgatg accacggata cctacctcgc gttcttcggc gagatatacg
     6541 gtatgccgaa ggcggccata cggacccggc gcgacgacct cctcgcgggg ctcgcgctcg
     6601 agccggatgg gaagaagatc ggggaactct cgaaggggat gaaacggaag gtcgccatcg
     6661 cgcgatcgct gatgcacgac ccgggtctcc tcatctacga cgaacccaca tccggcctcg
     6721 accccatgac gtcccggtcg gtgctcgagt acatcaaagg cctcagggac cgggggaaga
     6781 cggtgatctt ctcggcccac aacctctccc aggtggagga ggtctgcgac cgggtcctga
     6841 tcctgcgccg ggggcgggtg gtggccggcg ggaccatagc cgaactcagg gagaccttcg
     6901 gttcgatcac ctaccgggtc ttcttccgca ttccggacct ctcgtccttt gccccgccgg
     6961 tcgagtactc cgcccacgac ggccggtacc ttgccgaggc gcgctccatc gacgagttga
     7021 accggacgac cgcggcgctc gcggcggaag gtgccgggat cgaccggatc gagtcgcact
     7081 acccgacgct cgaggagatg ctcttaaaga tcgggcggtg atgggagcgt aagtccggcc
     7141 gcatactccc cctccccttc cgaccctgcc ggcgcccctg cggcgggcag gtgcaaaacg
     7201 gggaaccccg ggcgatctct gccatcctca cggaactctc ttgaattttt tgcctgagca
     7261 gcatttatat agcagcggca tatatgtgcc tccggaaccc ccgggaatcc gcaacaaccg
     7321 gccttctgtg agaaaccgtg accggggtga aggctgatga cggtcgaccc gatggccgtg
     7381 cgccccgccg gcaatgaaga accgcttcgg aagggcgatc gcgagaaccg ggcggtcgtc
     7441 gccagcgata tcgagtacag gtcaggaaag aatgagggtt cgacgggcct gaagggattt
     7501 gaacccttgg cctacggatt aagagtccgt cgctctgccg actaagctac aggcccctgt
     7561 atctgaccta aaaagatagt acgtgaagag gtataaacct tacggggtcc tcccgtaatt
     7621 cgccgatatg gaatccctta ataccatgag aagttacacc attgtacgca tgcctgaggc
     7681 ggtccagaac gtttcaaagg aacgtattaa gtacatcatc ttcggttgcg ggagcaccgg
     7741 ctataacgtc gcggaggaac tcgggcagga gagcgaggac ctcgtcatcg tcgataagga
     7801 cgagaagcgg gtggaagacc tccgggacca gaagtatgaa gcgctcgtcc gcgatctccg
     7861 cgacccgaac ctcatggagg ggttgcccgt gccggaggtc gcgttcatcc tcaccaacga
     7921 tcgtgacgcc aacctgaccg ccctcaagac catcaagaac cgctacccgg cgacatacgt
     7981 catcgcgcgc gccaccgacc cggtcagcgt cgacctcctc cagcaggagg gcgccgatat
     8041 cgtcctctac ccgcaggagg tggtcgcccg aaccgccatc caccacatca ggaaactcca
     8101 ctcgtcgagg cttgccctgc gcctctacga cctgcttgcc gcctgggagg ggacgctctg
     8161 catcgtcacc catttgaacc ccgacccgga ctcgatatcg agcgccatgg cgctctcgat
     8221 gatcgcccgc cacgcaagcc acaacaagct gaactgccgc atcctctacg agggagacat
     8281 cgggcaccag gagaaccggg cgttcatcaa cctcctcgag atcaagatgg agcggctcac
     8341 cccgcagatc cttagcgagt gcaactacat cgccctggtc gactcgtccg cgcccggcgc
     8401 gaacaacgag ctcccgaaga ccacccgggt caacatcatc gtcgaccatc acaagaacgg
     8461 cgagcgcccc cccggcgccg ccgacttcgt cgatatccgg cccggtgtcg gtgccacggc
     8521 aagcatcatg acccagtacc tcatggagct cgatataccg gtgagcaaat cggtagccac
     8581 cgcgctcctc tacggtatcc gggccgacac gcgggatttc aagaggaacg tcacccctca
     8641 ggacctcaac tacgccgcgt tcctcctccc cctgaccgac tcggacctcc tcgacaagat
     8701 tacctcccct tccatatcgc aggagaccgt ggagatcatg gggaacgcca tacggaaccg
     8761 gcggataagg agcggttacc tcttctcgaa cgtcggtttc atccggaatc gcgacgcgct
     8821 cccgcaggcg gccgatatcc tgatccatct cgagggggtg aacacggcgc tggtctacgg
     8881 catcagcgac cagaacatcg tcatatccgg ccggaacaag gatatcaggc tccacctcgg
     8941 gaacgtgatg gccgaggcgt tcgggcctat cggcgaggcc ggcgggcacg ccacgatggc
     9001 cgccgcgatg ataccgctca gctacttctc gatggtgaag gagaaggagg aactgctcaa
     9061 cctcatcatc gacccgatac tgaagcggtt cgcaaacatc gtcggcctgg ataacaacga
     9121 gggcaagcaa tgaggtttgc ggactgggaa cctcattaca ctgcaattct tgattatttt
     9181 gggtttgagc gcgatgcgga cgaggcggcc gcccgcctgc tcgaggagac tgccgacggt
     9241 cgtgacgacg tcggactcct cgaggcgctc atacggggca gggcggtgac ggtctgcgga
     9301 aacgctccct ccctccccga cgagctcgac cggatcgagg ggacggtgct tgccgccgat
     9361 gccgcggccg aggtgctcgc aggtcacggc atccggcccg acgcggtctt caccgacctc
     9421 gacggggcca ccgacgtctt cgtcgacctc tcccggcagg ggacggtgat ggtcgtgcat
     9481 gcccacggcg ataacatgcc gctcatccgc cactgggttc cccacctccc gggcccgctc
     9541 gtcgccacca cccaggcggc gccgttcgcc cacatccaca acttcggcgg gttcaccgac
     9601 ggcgaccggg cggtctttgc cgccgatgac ctcggtgcgg catcgatccg gatcatcggg
     9661 ttcgatcttg ccgataaaaa cgtcgatccc gtcaagcggg gaaagctcta ctgggccgga
     9721 gagctcctcc gcctgatcgg gtatgacctc taacgccctc atcatcgacg gctacgtcga
     9781 cgagcccgcg tgcctcggcg tcgcgcccta catatcccct tacatccgcg aggtcgccgg
     9841 cgtcctcgtc gaacacggtt acaccacggg ctacgtcacc atcgaccagg tccgtgccga
     9901 tccctcgctc gtcgccggcc acagcgacct cgtcgttatg atagcggggc tgaccgtgcc
     9961 cggcgcctac atcgggggga aacccgcgac cctcacggag atccagcagc tcgggaccct
    10021 gctccgggaa ccggccaccg ccataggcgg gccgatcgcg ttcggctacg cccccggggg
    10081 cgggaggaaa gccatccgcc aggcaattgc cgggtttgac gccacgctct cgggatcgcc
    10141 cgcggtggcg ctcgatgcct ggctctcggg cggagaacct gccggtgcag gcgactactc
    10201 cctgaccgat ccctgggccg tggcaggcgc cggtatcgtc gcccggcacc cggcgttccc
    10261 gtacgtcatg tgcgagctcg agaccgcgac cgggtgctcc cgggcgacgg tcgggggatg
    10321 ctccttctgc accgaaccgt tctacggtct cccgcgctac cggagcatcg aggggatcgc
    10381 gagggaggtg gcggcgcttc acgcggcggg cgcccgccac ttcaggcttg gccggcaacc
    10441 cgacctcctc gcctaccaga gccacggcgg ggagttcccg gcaccgcgcc cggaggtgct
    10501 cgccgaactc ttctcggccg tgcgggagag cgctcccggc cttgccaccc tgcacatcga
    10561 caacatcaac ccgggcacca tagcccatca cgaggatgcg gcgcgggagg cgctcggggc
    10621 gatcgtcgcc ggccataccc ccggcgacac cgcggccttc ggcatggaga ccgccgatcc
    10681 ggcggtggtc gcggcaaaca acctcaaagc catgccggac gacgtctttc gcgcgatcga
    10741 ggtcgcaaac gacgtcgggg gaaagcggac gaagggcatc cccgaactcc tcccgggcct
    10801 gaacttcgtc gtcggcctcg cgggggagac cccggcgacg ttcgatgcga acgaggcgtt
    10861 cctccggcgg gtgctcgatg ccggtctcct cgtccggcgg gtgaacatcc ggcaggtgat
    10921 gccgtttgag gggacggcgg cctacgagaa gaacaccctc ggtgcgcacg acgcccggtt
    10981 ccgcgcgttt aaggagtgga cccgaagaga gttcgacgag ccgatgctcc gccgggtctt
    11041 ccccatcggg acggtccttc gcgatgtcgt cgtcgagata gcgggcaggc cgtcgtttgg
    11101 gcggcagatg gggtcgtacc cgatcctcgc cgggattccg ctcgacctcc cggcggggac
    11161 ggtgctcgac gccgtggtgg tggactgggg gatgcggtcg gtgacggccc tcccctgccc
    11221 ggtggaggtc aacaccctcc cggtcgcggc tctccggtgg ataccgggcg tcggcaagaa
    11281 gagggccgca accatcgccg cccgccggcc gtttgagaat atcgcggagt ttcaaaagat
    11341 tgtgggggag acgggtatcg agggtgcgat gaagttttag tcctcctccc gcaactccat
    11401 cacccggagc acgtccgtcc gggtgacgat accgacgaga gcgccgtcgg aaacgaccgg
    11461 gatcctcccg atatccttgc ccgttatgat cttaagggca tcgaggagcg gtgccgacgg
    11521 cgggagcgtg gtcgggtttc tggtcatgac gtcgcgcacc tgcatggcct cccggtcctg
    11581 cggcgttatc ttatggatgt cggcgagggc gacgatcccc acgagcgccc cccggtcgac
    11641 cacgggaaag ccgagatgtt tcgtctcgta catcatctcc accagccggg agaggggcag
    11701 tgtcggctcc acggtggcga cggggctgct catcgcatcc gccaccgtga tgtcctgcag
    11761 cagtatgttg tagcggagga ccgtggcctc ctggcttgcc ccgatgtaga tgaagaaggc
    11821 gatgatgatc aggatggggt tgaggaagag gagcccgacg atcccgaaga cgacggcaaa
    11881 ccccttcccg acgtcggcgg cgatccgggt cgcccgggag agcggcatcc tccgggcgag
    11941 ccatgcccgg agaacgcgcc ccccgtccat ggggaacgcc gggaggaggt tgaacccgaa
    12001 gaggaggatg tttaagaggc ccaggtagcc gaaggtgaag accaggaccc cggcgaccgc
    12061 cgggtcggcg accgcggcgt cgaagacata gaccagggcg gcgctgatga ggccgatcgc
    12121 aaggctcgtc atcgggcctg cgagcgccat ggggagttcg atcttcgggt ccggcatcgt
    12181 ctcctccatc tgcgagacgc cgccgaggat gaggagcgtg atgctgtgga tctcgatgcc
    12241 ttttgccttt gcgatgagcg agtgagccat ctcgtggatg aagacgccga cgaagaggcc
    12301 gagagcgacg atgctcccga gtatataggg gttgagcccg gccgcaatca gcgtcacgtc
    12361 gatcggaacg ccaaagaggg tcgcgatcaa ctcggtcgtg agccggatct ggctcccgat
    12421 gatccaggca aagagaggga tcacgaggag gaaactccag tgcaatttga ccggaatccc
    12481 ggcaagtttt ccgatctgca gcgacgcatt catgggaaat gagtatcttt ttatcattta
    12541 tagtagtata ccttcagagg ataccgatga gaacagagat caccaacctg tttgaactcc
    12601 ccgtctatac cgaaaaggcc atctttgtcg ggaatgttga tgacatcgtg gtcgatgtgg
    12661 accaaaaaaa gatcgactcc cttgcggtcg ggaacctcga ccctgcgatc agggaggtga
    12721 tgaaggggtc ggggctgaag atccccttcc gcatcataaa gagcatcggg aatatcgtca
    12781 tcatccgcca tatcccgggg ctctttcggt cgccggcaaa agaggaataa ccgcgggatc
    12841 ctgcctgccg ggcgatcatg gatatcaacg atcgcgagat cttctcacgc ctcgccatct
    12901 tccccccctt ttttgtcagg ctcgacggcc gggcctttca ccgccttgcc cgtgcgctcg
    12961 agcttacaag gccgtttgac ccccggttcc acgcggccat gcggtcggcc tgccgctacc
    13021 tcctcacggg gagcgggctt acgcctctct ttgcctacac cttctccgac gagatcagcc
    13081 tctacttctc gacgctcccc ttctccgggc gggtggagaa gatcgactcc gtgaccgccg
    13141 catctgcggc aagcgcgctg acgatcgagc tcgggtgccg cgagcccctc gcgttcgatg
    13201 cccgggtcat acccgcggca ggggagtttg cggtcgagta cctcgtctcc cggcagaacg
    13261 aggcgtggcg caaccacatc aacgcctact gccagagcgc gctggtcgat gaggggatga
    13321 gcccccgcga tgccgccgcc gctctccggg ggatgcggtc ggaggcgatg cacgagatca
    13381 tgttcgagcg cggcgtcaac ctggcggcga cgccggcctg gcagcggcgg gggacgctcc
    13441 tctaccggga ggagtgcata aaagaggggt ataaccccct gacgggggag cgcgtcgagg
    13501 cgacgaggac ctgcatccgc gaaccggagg agacgcccct cttctcaacg gtcgagggga
    13561 cggcctttat ccggtcgctt atcggcgcgt gagatgccga tccgcatctg cacgccttcc
    13621 acgtcccagt ccttctcgag agcaaagggt tcggccggcc tctctccatc ggcgtggcgg
    13681 acggtgagga ccgccgcccg cacctcgccg gctatctcgt ccttccactc ctcctccccg
    13741 atgagcgcga cgattcgccc gtcggcgacg tggacgtcgg cgacgatgta gtcgtcgacg
    13801 ttcaggtcga gctggcggcg catctcctgg atccgccgga tcacctcgcg ggcataccct
    13861 tcggcctcga gggccggtgt gagggcgacg tccacgtaga ccgtcgcatc cttcatgggc
    13921 gcggcaaaga cgcccgcggg gagggcctcc gtgaaggcga cgtggcgcgc ggtgatctcg
    13981 tagccgtcga gttcggcctt cccctcccgc tcgatggcgg ccttcagggc ggtgccgtcc
    14041 gccccctcga tcagcgcttt gaccttcggc ccttctctcc cgaactccgg gccgattgcc
    14101 cgcatgaccg gtgcggcctg ccagaggatc cgctcccacg tccccgtgac gaccctgacc
    14161 gcccgggcgt ttgcccgggt ccgggcaaga tcgtttaagt cctggagagc cgtcttcacc
    14221 tcgtcgcttc cggtgaccac aacggtctcg gagaccggcc agcggagttt ccgttttccg
    14281 gcctgccggg cggtggcgac agcatcgtca aacgactgga tgacccccat atcggcctcg
    14341 agggccgggt cgatcaggag atcgtccgct tccggccagt cgagcatatg cacgctctcg
    14401 gggtccccgg cgaggcggag attctggtag atctcctcga cgatataggg ggtaaacggc
    14461 gcgaggagcg ttatcaggcg gcgcatgacg tagtagaagg tctcgtaggc ataccgtttc
    14521 tccggcgagt cttcctcgag ccacatccgc ggccggacga gctgcacgta ccagcgggag
    14581 aggtcctcga ggatgaaggc ggcaagcgcc cgtgtctccc ggtgaaggtg gtactcctgc
    14641 atatcctccc cgatcgcccg gatgagcgtg ttggcccggg agacgatcca gcggtcctct
    14701 tcgggcatat cccggatgtg ggaccggacg aacgagtcgt cccaccgctc gccctccccg
    14761 gcggcgggct ggaatgaatc gagtatcatg tacgggagcg ggaaccggta gacgttccag
    14821 aggatgttga gcgtccggtg gatcgtcttc acgccgtccc agttgaactt caggtcgtcc
    14881 cagggagcgt tcgcccagag gacgtagaac cgcagaacgt cgacgccgaa ctggttcacc
    14941 acctcttcag gggtcaccac gttgccgaag ctcttgctca tcttgcgtcc ttcggcgtcg
    15001 agagcaaacc cgtgcatcag gacgctcttg tagggggcgc gggcgaacgc cacgttgctt
    15061 gccccgagct gggagtagaa ccagccccgg gtctggtcct gcccttcggt gatgaagtcc
    15121 gccggccaga ggcggtcgaa ggcctcgcgc tcccggggga acccgagggt tgcccaggag
    15181 gcgaccgccg agtcgaacca gacgtcgaat atgtcttcca cccggcgcat ctctcccccg
    15241 caggggcagg gtatgacgac ctcgtccacg taggggcggt ggggatcggg aacctttgcg
    15301 cccgatcgct cctcgagttc ggcgatggtg ccgacgaccg tatactcgcc gcagtgctcg
    15361 cagtgccaga tgggtatggg gataccccag tagcgctgcc gggatatgca ccagtcacgc
    15421 gactctttga cgaagtcgtg gaaccgggcg ctccccgccc agtccggata ccacttcacc
    15481 cccgcgatct cttcaagcat cgagtcgcgg atgacggtgg ccttcaggaa ccactgcgcc
    15541 gtcgcccggt atatgatggg ggtcttgcac cgccagcagt ggccgtagcg gtgggtgatc
    15601 gtccgccggg cgaggaggta gccgccgagc gcgtcgatga cgatctcgtt tgcgtcccgg
    15661 acgtacatat ccgcgaacgc cccggcggcc ggggtgaagc atccgccgga atcgaccggg
    15721 cagaaagcct caagcccctc ccggatgccg atgaggtagt cgtcccaccc gtgcccgggc
    15781 gcgatgtgga caaggccggt gttctcgagc gcgacgtaat cggccgccac gacccggtgc
    15841 cggatctccg cctggtgcgg cacgcacccg gcgagcggcg atgtatactc cgtcccgaca
    15901 aggtcgctcc cgctcacccg ctcaaggacc gtgtagtcct ggtagcggcc catcttcagg
    15961 acgggctcga tgagttcgtc ggcgatccag aggatctcct cggcgtcgtc cttctttgcc
    16021 ttcacccgcg cgtagatgaa cgcggggttg accgcaaccg ccacgttcgc gggcagtgtc
    16081 cagggcgtcg tcgtccagat cacgagatac tcgttctcgc gcccgctgag cgggaacttg
    16141 acgaagatcg agggatcggt ctcgtcccag tactccacct ccgagtcggc gatcgccgtc
    16201 tcgcaccggg ggcaccagtt cacgacccgg tggccgcgtt cgaggagccc gcgctcgtcc
    16261 gcacgctgga gcgtccacca ggcggactcg atgtagtctt ccttgatggt ctggtagggg
    16321 ttgtcgaagt cgagccagac gccgagacgc cggaactggt cgctcatgat ccccatgtgc
    16381 gtcacggcaa acgtccggca ctgctcgacg aatgcggcga tgccgtactc ctcgatatcc
    16441 ttcttcgagg tgaacccgag ctggtgctcc accttcacct cgatggggag gccgtgcatg
    16501 tcgtagccgg cccgctcgat gacgtcggcg ccccgcatcc ggtggtagcg gaggatggta
    16561 tcttttatga tcttattcca ggcggtgccg aggtggatgt ggccggtggt atacggcggc
    16621 ccgtcgacga aaaagaacgc ttttccaccc tctcgtaacg ccctgacgcg tgcgtaggta
    16681 ttctctttcg tccagtagtc ctggactcct gcttcgatgt cacgcgggtt ataactgctg
    16741 gtgacttctt tcacctcata tccctcatat ccaacactct ggtgtaccgt ttgttctccg
    16801 gggacaaagt atttgtgcgg ctcacgcacc cccgagagcc tttatacccc cggcccccta
    16861 cctccctgca tgcagatcgc ggtcgtcggg aggggggact gttccggcga ggagtacgcg
    16921 gcggcccggg ccgtcggccg cctcatcgcc ggcatcggtg cggttgtctg ctgcggcggc
    16981 cttggcgggg tgatggaggc cgcctgcagg ggtgcacggg aggccggcgg gacgaccgtc
    17041 ggcatcctcc cggacaccgg ggacgcgaat ccctatgtcg acgtgacgat ccgcaccggc
    17101 atgggccacg cgaggaacgt cgtcctcgtc cactcggcgg acacggtgat cgccgtcggg
    17161 ggaggttacg gcacgctctc ggagattgca gtcgccctca agacgggaaa accggtctac
    17221 ggccttgcca cctgggagat agagggggtg gtggcctgcg cgacgcccga ggaggcggtc
    17281 agccgcgcag gacgcccgtc tcgtccgtct cgtagccccc gaggtcccgg agaacctgct
    17341 tgaacgcagc cgaggagacg gtctcgacga gagcggcaat ccctgggtcg tcgagcatcg
    17401 cgcggtgcat cgcgagttcg tagcgctccg tcgccaccgg gacgaacgcg agatcgagcg
    17461 cttttgcggc gctgtaaacc cccattcccg catccgcctc ccccgtccgt accgcgaggg
    17521 ccaccgcaag gtgcgtcgtc gcctcgcgcc cgtagccggg gatggacgcc gggtcgatgc
    17581 cgcgcgccgc aagggtgtgg tcgaggagca tcctcgttcc cgaccccttc tgccggttga
    17641 tgaacgtcct gccgggcagg tcgtcgaacg agaggccgtc gcgggatatg accccctgct
    17701 gccgctctgc cacgcagagg agggcgaggt ccgccccggg catgtagcgt tccaggtagt
    17761 aggtgttgta ggtgccgtcg ggagcaagca ggtgcatcgg ggccgcgtgg cactcctcct
    17821 tcttcagggc gatcaccccg cccatgctcc cgaggtgcgt ggagtggatg tcgacgccgc
    17881 gcggccggac caggtcggcc aggtagtcga gggccgggtc gtggctgccg gtgacgagca
    17941 ccgcctcctc cgcctctgcg cgcgggacgg tgagccggac gtccacggtc tttcccgcct
    18001 cggcgccctc catatcttcc gggatccgga ggtagccgtt cgcccgcacc gcgctcatct
    18061 ggatacccgc gccccgtgac tggggcaccg ccacccagcg gtcgccgata tttcccaccg
    18121 agaggaggac gaactcgtcg gtcccgatat ccgactggag ggtcgtggtc agccgggcct
    18181 cgacgcgctc cccctcccgg gtggggagac cgtagcgggc gacgagcggc aggacgatct
    18241 cccggagcac ggtcgccgcc gcaagaggat agccgggaag cccgatcacc ggcttcccgt
    18301 cgatccggcc gatgatcacc ggctttccgg gcttgatggc gaccccgtgc gccagcactt
    18361 cgccgaggtc ccggacgatc tctgcggtgt agtcgcgggt cccggcggac gacccggccg
    18421 agacgagcag gatatcgttc tcggcgacgc cgcgccggac ggcctcccgg atcaggtcgt
    18481 agtcgtccgg gacgattggg tagcgatgcg cctccacgcc cgcctccgtg aggaccgccg
    18541 cggccatcag ggtgttgctc tctacgacct tcccgggcgg cggcatctca cccgccggca
    18601 ccagctcgct cccggtgggg atcaggccgg cccggacgtt ttcgaccgcg acctccgtca
    18661 ccccgtagtt tgcgagcgct cccacgtcct ggggccggat ccggtggtgt gaggggagaa
    18721 tcatctccga ttctccaata tcctcgccga ccgggcggat gtgctgccag gggctgaccg
    18781 gctttcggac ggtataggtc tcgccgtcga cccagacgtc ctcgatcatc accaccgcat
    18841 cgtaaccggc cgggacgatg ttccccgtgt tgacccgtgc cgcgtcggga agggtcaccg
    18901 ggttctgctc gcttgccccg agggtctcgg cgctccggac cgcgatccca tccatcgccg
    18961 ataggtggat ggccgggacc gagaaccggg cgaatatggg cgcggcggtg atcctcccca
    19021 ccgtcccggc ggtcaccgga acccgcacga cccccgggat cgccggaaat gagcgttcca
    19081 cgagtgcccg ggcttctgcg agcgatatga ccgagaggta gcgcttcacc acaggatcac
    19141 ctccacctct tcgccgacct cgagcccctc gctcgatgcc gggacccgca cgatcccgtc
    19201 gctctgcacc agggtgttga ggaggccgga cttcccgaag atcggcacgg cctcgccgtc
    19261 cctgacgctg acccgaacgt agtcctcccg cccgcgcgtg gagggtatgt tctgcttcag
    19321 ccgtgcggag agggtccgcc gggagaccgc ggtgccgcgc atcgccgcga gcaggtggtc
    19381 gacgatggcg acgaggacga cgaacgtcga ggccgggtgg ccggggagcc cgatgaccgg
    19441 tcttccccgc gccgtcccta tgatggtggg cttcccgggc gcgagggcga ccccgtgcac
    19501 cagcacctcg ccgaggtctg cgacgaccgc ggcggtgttg tcccgctcgt ccttcgagga
    19561 gcctcccgat accaggacca cgtcgcacgt atcaagcgcc gatgagactg ccgctttgag
    19621 cgcctcccgc tcatccctga cgatgccgaa gtagaccggg cgacaccccc tctccgtcac
    19681 gaaggcgccg gcgaggtagg agtttgcatc ccgcacctcg cccggccccg gcgtcgcggt
    19741 gacggggacg acctcgtttc ccgtggatat gatgccgacc cgcgggacag ccgccacggt
    19801 gacccggtcg gcgccgatcg ccgcggcaac gccgatatcc cggggcgaaa ggacgcggcc
    19861 ccgctcgagg acgaccgtcc ccgcccggaa gtcctctccc cgggtgatca cgttctcgcc
    19921 gggtgccact gagcggtgca cgaggacatc gtccccgatc tgctcggcgt gctcgatcat
    19981 aacggccgca tcggcgccgc gggggagggc gccgccggtg gggacgtaac ggcaggaccc
    20041 gggtgttatg ctcccggcat ccgcgctccc cattcccacc cggccccggc acgagagcat
    20101 cgccggtata gcctcggagg cccccgtggt atcggtcgcg atgaccgcgt agccgtcgac
    20161 cgtcgaccgg tcaaaccccg gtatgtcgat atcggcgcga atatcctccg caaggacccg
    20221 gtggagggcg ttctcgagcg ggacatcccc ggactcgagc gggggcgcga tccctcgcac
    20281 cacctccacg gccttgccga ccgagacgac cgataaaaag aggctcatct gaagcagccc
    20341 agctgacagc tccggatctt gatcctcggt tcgcgctggt tgcagtaccg ggcgatatcc
    20401 atcttcggta tgttgtactt ctccgatatg tcgaaggcct gggggcaggt gatctcatcc
    20461 gttatgccgt agtcttcgaa tgcctttcgc accttttctt catccatagt actcacatgg
    20521 tctgcctgca aagagatagg atttactgta gggcatgtca agcactatca tttagcatgc
    20581 tgccggccca cgtatactga tgcggatcgc gctgatcaac tcaagagtag atgccgcggg
    20641 ggtaaacatc cgtgaccacc ttggggttct acttacggag ggcgggcggt ggccgcttgc
    20701 ggatgaccac gagctcgtgt ttcacgaggt cgccggccgg ctgatctacc aggaccggat
    20761 cgacgaagag gtcgacgccg acctcatcgt attcatatcc cgccacgcga gcgcgcaacc
    20821 ggtgcccgcc ctgaccgtgc acgtgacggg caactatgag gccgccgatc ttggcggaga
    20881 gcccggagcg cttgcgccgg cggcccctgc gtggatgcat gccattctcg ggaaccttgc
    20941 cgcccgggcc ccggagggct accgggtctc ctacgaggtg acgcaccacg gcccgacggc
    21001 gctcgcgaca ccctcgctct tcgtcgagat cgggtccacc gccgccgagt gggccgaccc
    21061 cgccgccggg agagcggtgg cggagagcat ccttgccgcg gcgccgcagg agacgatcga
    21121 cctcatcggg tttggcggaa cccattacgc cgtgcggcag accgccatcg cccttgcctc
    21181 ccggggcgct ttcggtcaca tcgcgccgac ccggcagatc ggcgccctcg attccggtcg
    21241 tatccggcag atgcgggagg caagccgcgc cgtcgccgcc tacatcgata aaaagtcgct
    21301 ttccgccgac gaggccgccc gggtcgagcg gctgctcgac ggcgccggta tccccctcct
    21361 cacggagtcg gagatccggg agatcggaga cctcgaatgg gccacctacc tccggatacg
    21421 ggctcttgcc gatgagatcg cccccggatc ccgcgcccgg atccacggtc ttgccgggca
    21481 gggaacgccc gtgccggtcg atgtgaaccg agatctcgtc gaggagacgg caaaatccga
    21541 taaaaccggg tttataaccg cccttgacgg gttacccgtc gcccatctat cgaaaggttc
    21601 aacagaggtg ctgccgacgt ttatcgggtt tgtgtacggg tcttcccgac tcgcaagtga
    21661 tataactacc ctctgcgtaa aactcttact tatctctgaa gacgctgtta tcgacggtga
    21721 tcacctcgtc ctccggaagg tgcggttcga ccctgagaag gcgcgtaggc tcggggttcc
    21781 acgggggccg ctctttgcca tgcttgccgg cgggaaggcg gttgagatcg gaggacaaac
    21841 ggttacgccc gatgcagtac aggcaaccag tataaagagg atacacatcc cgggattgga
    21901 gagatacata tgaaatccat cgtagaagag gcactttcac gggcacgaga ggagcagcgt
    21961 tttgctgagc ccacgggagt cgacaaagat atcgaagacg tcctcatgga actccggacg
    22021 gagattgcgg tcgtcgggtg cggaggcggc ggttcgaaca ccatcacccg gatgtcggag
    22081 gagggtatca acggggcgcg tctgatcgcc ctcaacaccg atgcccagca cctcatccgg
    22141 actcatgcag agacccggat cctgatcgga aggcagagga cgcggggcct tggcgcgggt
    22201 tcgatccccc aggtcggcga ggaggcggcc ctcgagaacg aggacgacat caagcgggcc
    22261 gtcgaggggt gcgacatggt cttcatcacc accggccttg gggggggcac cggcacgggc
    22321 tccgccccgg tggtcgcgaa ggctgcccgg gaagagggag ccctgaccat cgccgtggtc
    22381 accctgccgt tcacggcgga aggcgccatc cgggcacaga acgccgaggc gggccttgag
    22441 cggctccgcg aggtggcgga taccgtcatc gtcgtcccaa acgatcgcct gctcgaggtc
    22501 gtgccccgcc tccccctcca cgccgccttc aaggtctccg acgaggtgct gatgcgtgcg
    22561 gtcaagggca tcaccgagct gatcacgatg cccggcctcg tgaacctcga ctttgccgat
    22621 gtccggaccg tcatggagcg gggcggcgtc gcgatgatcg gtatgggcga gagcgacagc
    22681 gaggacaaag ccgtcgattc ggtcaagaag gcgctgcgct ctccgctgct cgacgtggac
    22741 atatccgggg cgaccgctgc tctcgtcaac gttgtcggcg ggccggacat gaccatgtac
    22801 gaggctgaag gcgtcatcca ggaggtctac gaccgcatcg acccggatgc ccgcatcata
    22861 tggggcgctc aggtcgatcc cgatatgcag ggccggatgc ggacgctcct cgttatcacg
    22921 ggtgtccggt caccacaaat atatgggaga aacgagaaga gtatgccac
//
LOCUS       DAOSEE010000017        22770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1003_length_22770_cov_234.748668, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000017 DAOSEE010000000
VERSION     DAOSEE010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 22770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22770
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1003_length_22770_cov_234.748668"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..1661
                     /gene="serA"
                     /locus_tag="PLI76_02410"
     CDS             78..1661
                     /gene="serA"
                     /locus_tag="PLI76_02410"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOI60913.1"
                     /translation="MKYRVLVSDPLAEEGIDILKEFGDVDVNTGLTEDQLVAVIGDYD
                     ALLVRSGTEVTARVIDAGAKLKFIGRAGAGVDNIDTEAATRRGIVVANAPEGNTLAAT
                     EHTMAMMLSLARNIPQATASLKKGEWKRSKFMGVELNDKVLGVMGFGRIGREVAKRAQ
                     AMEMKCIAYDPFITKEKAATLGVEMVPLDELFRRADVITVHTPLIKETRHVINAETIA
                     TMKDGVRLINCARGGIIDEQALADGIASGKVAGAALDVFEAEPPTDSPVIGLEKVIIT
                     PHLGASTVEAQKNVAISVANQCISVLSGGAAKYVVNAPMIPAEQQAVVEPYAALAQKM
                     GSLLIQLIEGRLESIKITYGGEAATLPNTKFITRVILKGMLDPILQAPVNFVNAEFVA
                     KERGIRLSETTTEEAHGFRNMISITAKTDRMSESVSGSVSAPDHARIVSIGGYMTDLT
                     PTGHVVISRHTDKPGVIGKAATILGRVNVNIAGMQVGRHKPGEEALMVLTVDSAVPAE
                     AMDEIKKIDGIHTAKHAEI"
     gene            1796..1869
                     /locus_tag="PLI76_02415"
     tRNA            1796..1869
                     /locus_tag="PLI76_02415"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1830..1832,aa:Val,seq:gac)
     gene            complement(2930..3409)
                     /locus_tag="PLI76_02420"
     CDS             complement(2930..3409)
                     /locus_tag="PLI76_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOI60914.1"
                     /translation="MTMEFGTITRDDLPELVRLQIDLIEEIRDETGDDYFLYDTERAS
                     EIAEEIASIFDNPRKIIYIARDGDCIAGFIAGEVRSCFFPLSKAGPVGYIAGAYVRPM
                     YRKKGVARRLEEMLVRFFREHGAAYAELGVMSKNRLAKKSWNSLGYATFRELMKKQI"
     gene            complement(3542..3829)
                     /locus_tag="PLI76_02425"
                     /pseudo
     CDS             complement(3542..3829)
                     /locus_tag="PLI76_02425"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
     gene            complement(3879..4661)
                     /locus_tag="PLI76_02430"
     CDS             complement(3879..4661)
                     /locus_tag="PLI76_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60915.1"
                     /translation="MKPNAPRRRIEDGADKKPGSHGNHWGCVTQSTDEIIRSVEWALK
                     EAKNPDIRAWNGREVAAYVPESRSLTTCAITLDRSVSTGYPMAVDGVVNEVTVDRIEE
                     WANGLEGQVVGSIGDAALAFFDTRYYRNIGRYRTGGRHPFALAAFAYVLHKAEPRTIT
                     DAEGKEHSTRNLAAYRPFPQGDVDDFVYQSPVKSVEAVSFCGKTVYRLTVPLFRLPDE
                     GGEYHDIDITLYAAGHVTGGYAPRVGDDVMGILWLQGSLASG"
     gene            complement(4701..5909)
                     /locus_tag="PLI76_02435"
     CDS             complement(4701..5909)
                     /locus_tag="PLI76_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60916.1"
                     /translation="MFTIEWDTEEVRKDVVRISSLSPRYIDCIDLCTSICVSVSSVDI
                     AGLAENPAGNCTRLKHFFLDNFSVFRSIDPKRLRDKPFHRYYNVDNCVLGEGFRYYIL
                     YDRTSDLVTFEYIYTGKKVFQTLQIPLRAFANGVILSAEEMIVEILSVSTLGEDAGYW
                     IFKADLDIIKDWYREVYGVDADQDHPSPTPAPERQMPGIIAIDHPGAVQMVWNRKELE
                     DRIREVSATPYRSDIWKGFEMVFHFFIDDVDIIGGALTYFDSLLVNINWVFHRLDPEH
                     IPQEKTHFTSDEPEAKVSGTVFEFTVTLSDDRETVLIDYMSAQMNYYTRTTVPLKRFA
                     RAVVQANEEFLQEAREIWPEVVLTTYYQWLVDDIVTLREWYRERYHEELPPRNPPTRT
                     LRRNILHSIE"
     gene            complement(5919..6674)
                     /locus_tag="PLI76_02440"
     CDS             complement(5919..6674)
                     /locus_tag="PLI76_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60917.1"
                     /translation="MADIRDAVQAILNGDPLGAAMNAAGAIPGPGDGIKITGAIVCYT
                     GKFPWKARELGVLLSKELLGPLPDTIKIQVWDLLFDGAGSRLVRGEITPDHLLEMTKR
                     GVDLADVVRITKVGQDAIPLTKTQLDHIVNRHITGTEGIGDPTTLFPTSEPVVWSTVT
                     GISLQYPGTSTLTREEMREKIVDWIDEALRATQSEWPKQPKNLAYSPPDAHQYGIDEI
                     VIGINQKTGVGTFYPSKGPQVFRWYNKKWNPTV"
     gene            6852..6998
                     /locus_tag="PLI76_02445"
     CDS             6852..6998
                     /locus_tag="PLI76_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60918.1"
                     /translation="MRRQRVGVGAVEEAVAVGIRVEGIGAILEFCQIAQPVVGVGKVQ
                     IDSV"
     gene            complement(7026..7637)
                     /locus_tag="PLI76_02450"
     CDS             complement(7026..7637)
                     /locus_tag="PLI76_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60919.1"
                     /translation="MDEKLIDKIERGLIYYEKRIGEIDEHYDELGGRYARYSAGAPYF
                     YKHLLPFWMHLLEEGGVDCEGFRKEYARITRKLDTLERKRGRDYREKLLDRLNDDVDF
                     YPVIIYDFGELEPYELEIPNDLSTRTAIEIYMIELEREYDLTDIKQKVSTLDEALKSK
                     YMQNVEEILETYFDAEDPYSPDSLWWNHPLKLLQEKQAVQNNP"
     gene            complement(7657..8160)
                     /locus_tag="PLI76_02455"
     CDS             complement(7657..8160)
                     /locus_tag="PLI76_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60920.1"
                     /translation="MTRYSDETEKALSILWYSYGDDVVDVLKAHGTTDATLVRLYGGN
                     VNLFRVRHMLENVCEGGWAPTKKLTASGSLDKHYTDHVLGQFEWGETFTKSVYLQKAT
                     TLSNRRDHTVELYYQMGYDTLVVYDRGLGEFVSITKDGLITTFFKPKIDPHYVDETIA
                     DRLIRLN"
     gene            complement(8391..8858)
                     /locus_tag="PLI76_02460"
     CDS             complement(8391..8858)
                     /locus_tag="PLI76_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60921.1"
                     /translation="MRKEVIEKLKFLVFIRGVEPETEYMGKIYTQYTLVEFANGQNAR
                     LFDHKMLCTADMIGKQQKVALWMLVQSLETIEAEDKRVIPDPSEPSLDIYGCIEEIAI
                     PEEQKDAERWHHAIVNFGVGKTLTDIDKNYFRLNLQKGDFVHISGRVDLIAVE"
     gene            complement(8860..14298)
                     /locus_tag="PLI76_02465"
     CDS             complement(8860..14298)
                     /locus_tag="PLI76_02465"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HOI60922.1"
                     /translation="MVTDSGKIRLKTYEPETRTICITDESRSAADADLEIRLVNATPD
                     LGTFTEIFEITAHRTMILDGSEDFLATWSLQNGNENITRAEWFVWEERSHSIEIPIIE
                     YRDIIPGNVVSENVAYEDVIPETVALENVPFEDEVPINATPEPLPPEEALPADTALET
                     FVAQEPYIAGTETVERSWPEWVPFTPDGQTLNAGKTRLFKVIYTKPAETGRVDINTAP
                     IFRGVACPELTWWSTAWATRVPVTITTPPSVDGYPHREVVLFRSGMQSDFDDVRFVAD
                     DGTVLGYWKETYTASDSAVIWVNLPAGATSIWMYYGNEAVIDTGNRDNAYILYDDFDG
                     PDLDTTLWTDTIPGAHYFVDGWIYDTRAQGAGALVSNEPVITDGEPVIVEMHIHADSL
                     PSNAGYSCGAFPWFVDTPTNGLLWYPESPSWDRYVYMNGAAAWRPGTARGLSAGGDYS
                     STWIITPTSQTHTVSGSASYTDVFTGATGISSHQMQILGGNNAPEGRVVRMDWIRVRK
                     YVETEPAFTYGAPQTVPVVKTVAVTPSIVEVVEGETIQFTATAYDQYGAAMEDIAFNW
                     VSTNEMVGTVTSDGIFSARAAGTTTISAVTSTGGSVWGIAKVTVIHTVTPGSAWNWST
                     DGWTGWAHTASWTQPTGSCSEYGPVMVGSHGEHGADVHLSAGAVIGTVEHEFTDPSGV
                     GWDSLTLVARVPGTDIPQGRWMTIEVNDEVVYAESGFSGSDPENFLPKEFHADFPQSG
                     TVRVKVSHGQNPAWGTHFSMEYYSLELHPPSSAAPSITLTSPIDDAVGAGGTVTVAGN
                     VSDPAITSLTLTHNGVSSTIPIKDGNFSAEVTLADANTIVVSGVDSQGAPLSATLLLD
                     GDMLPAAFEQAIGFDPLNADSDCSQWTGNQSGNGVIDGYEVFAGDLPVFAKARIGADP
                     FVVDTDADGLTDSFELLKLGTFPEEPGMLSAMGGPIVAAGTDDDPDGDGLSNLEEQTY
                     GTDPLVADTDKDGLSDADEILRGTDPRAADTDGDGLADGAEVALGTDPTIPDSNDNEV
                     PDGAEDYWSGDRFLDDTLDLTVFGRGYAVANVSVAAVNYTHLISDEILVSKVCNIGFG
                     DDVETGTVAIAYDPTHVVDPSRLSIYRFDEDLGTFVNVTSTVDAVNGVVSCAAAGSAK
                     YAVLDSARWDALFADDESIESLGETDIYHVLLWVKDSQTGEGVGNASVTFWGVMGSQD
                     LVTKTHETGDRKGLTRNFAEYPTSGTKAYSIEKDGYKDHNGMFRIEPDVFSYTATSRI
                     HRVDAPTGFLTVTSTPTGAIVYIDNIYYGSGVSNVELLEGEHTLVLTTPSAYYGTAIE
                     TVEITRNQNTQIHVNLPPTFSCGDLRVICPEPGAAVSIDGFSVGTIPVSGEFTKTDVA
                     SGTREIAVTKEGFVPYSEYVTVVAGQTSTVNAVLVPEDLDGDGLPDIYENGYRDGFGN
                     WHAPDPYAVDTDGDGLSDGYEAGELVTEGGKTYYKQRSDPTKADTDGDGLDDYLEDAI
                     ESDPLNPDSDGDGLSDALEWEIGTDLWSADTDEDGHSDYEEWYDPDYDPLVYEERFSS
                     LVMGREFLLGAVLGEWGVDDHDNLFYLGGWIASGVIVIGDLRDIAATISRGDLVGTGL
                     NLAALIPGYGDAAKVAAVVGKFVLKHPELLKPAMVLLVGVAPHVDEAAEWASNFRKIH
                     GDEAVNKFLINGITESDLNVIAKNRGNLLEALEVVKRADGKVVWLEEGLTKAEAITLG
                     VKPSGWKHIKEKHILDPRGNQFVKFFGQEFGTDSAVKELIMEGAKYGQKVNEDGLFEY
                     VEPNSGKILHLVIGDNGYIVSAFPFGAK"
     gene            complement(14327..14731)
                     /locus_tag="PLI76_02470"
     CDS             complement(14327..14731)
                     /locus_tag="PLI76_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60923.1"
                     /translation="MNTTTARRQNAWIFFILKSLLVVPPVQRVITDEHERYGESYFSS
                     HGGVDDNHGVHSCRERRGIECNYIEHGYCRRLVPRQDIFWLGYAQSSIQYCSISGKSI
                     IVVGKELVPTHVMDQHFYLHFCRNSGYWRIRY"
     gene            14954..15226
                     /locus_tag="PLI76_02475"
     CDS             14954..15226
                     /locus_tag="PLI76_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60924.1"
                     /translation="MWIIALCGRCLIYTGCRIRIDGVALPSPGIGDLCISYRTPGLRV
                     DVGEYGSYSIIFSKGDVYEMFITVGPSSDYELLKDLAERAAAKLPQ"
     gene            complement(15254..16372)
                     /locus_tag="PLI76_02480"
     CDS             complement(15254..16372)
                     /locus_tag="PLI76_02480"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOI60925.1"
                     /translation="MVLVVWFREVDYMRGVAALAVIAVHVSMNYTRIPDVNLLALTNV
                     FIYIAAHFAVPVFIFISGWVLAARYTGEFSIREFYQRRAKTILIPYLFFTALYLLIAV
                     EGTIGFAGIPSPERVAEALLLGTGAYHLWFFALIIQLYLLFPLIVRGYGLFDRFGAAL
                     YLLLSLLFLLALWNTGAHLMGAYVGPEWYTVLIRLFPSHLFYFVLGIHVGRHTDRFRS
                     TLRSLSPAWVLAAAGGGALLIGGVWVAAVALSGSLAGTSLAVFCIYRILEPLYYVPVI
                     AVLVLAAWLLDGGGGEMAGAARSFGDHSYGVYLIHPLVIAAAATLWISLTGLSWADGL
                     TYPVIFVVAAVVSYAVVRAVSRVPYAGYVLGESRVRRG"
     gene            16485..16949
                     /locus_tag="PLI76_02485"
     CDS             16485..16949
                     /locus_tag="PLI76_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF296 domain-containing protein"
                     /protein_id="HOI60926.1"
                     /translation="MRVESMQYSEGQVGRVFSVRIDDGEDFIREIERFVAAMNIQSGM
                     VHFLGAVRNATLVTGPKDAVIPPTPRAEEIFGGWELFGVATIYPGVEGPSLHIHSAAG
                     KGIRSLTGCLREKAEVYLVIEAVVMEFVGISARRLPDEATGVNLPVFDRVLP"
     gene            16946..18784
                     /locus_tag="PLI76_02490"
     CDS             16946..18784
                     /locus_tag="PLI76_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOI60927.1"
                     /translation="MSGQPGFLPMTMAEAERLGIDRFDIILVSGDAYVDHPSFGTAIL
                     GRVLQDAGYSVGVIAQPDWRSDADFRRLGEPRLFFSVSAGNVDSLVNAFTPNLKRRSS
                     DAYSPGGRLLRPDRATLVYTDRVHALFPKTPVVIGGIEASLRRFAHYDYWSDTVRQAI
                     LADAPADLLVFGMGEHQVIAIADRLAAGEPVGSLTDIPGTAYRVDLKTWRGMDPAGYV
                     VLPDYAEVKSDRYAYARAFALHYNEQDPFRGRPVAQPHPKTVVIQNPPAMPLSGVELD
                     RVYELPYTRRAHPSYTEPVPALEPVRFSVVSHRGCFGACSFCALTHHQGRIIQSRSVD
                     SIVREVERMAKMPEFSGVVQDVGGPTANMYGMHCGRWETAGTCPDRRCIDCPTLDRSH
                     TEQLRLLERLCEIPGVKHVFIASGIRYDLIGPEDPDYLAAVSAHHVSGHLKVAPEHVA
                     ERVCARMGKPPRETFDRFREQFEALEKGKKKRQYLVPYFMSGHPGCRMEDMVELAEYV
                     RDTGLYTEQVQDFTPTPMSISTTIYHTGLDPFTLEEVYVPKGREKRVQRALMHYRDPE
                     NYALVCEGLRAAGREDLIGNAWRCLVPARPGGGRLTRSGSSRRQTR"
     gene            complement(18747..19025)
                     /locus_tag="PLI76_02495"
     CDS             complement(18747..19025)
                     /locus_tag="PLI76_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60928.1"
                     /translation="MICRRRVSEVGEWRSTTMKTVSDVKERFALSPPPLPDGWRSSTM
                     RTLDAVVTPGTTANFAATLAVATLVIPRIPFRPLAVATGFVAGSILNG"
     gene            complement(19243..19548)
                     /locus_tag="PLI76_02500"
     CDS             complement(19243..19548)
                     /locus_tag="PLI76_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60929.1"
                     /translation="MRPIILVLLLIAVAGFAGTEVINVSLPDRDAPEAVLGTAESMWG
                     FVEWLLQVADDLLSLVRDVLGYLEVGGEYVERLMGAMDSGKDLVNRMLEEGAPSRGL"
     gene            19752..20444
                     /locus_tag="PLI76_02505"
     CDS             19752..20444
                     /locus_tag="PLI76_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HOI60930.1"
                     /translation="MQYEITGDNLQIVTLRLAPGESICAEAGAMVNMSGNMQMTTNMK
                     GGLFKGLKRMVTGEGLFMTEFTPQGGEGFVSFAGNVPGKIFTLNLAGNEFIAQKDAFL
                     CSEQGIDLDIAFTKRLRSGAFGGEGFILQRLSGSGTAFLHCCGDIMEMTLAPGEVVKV
                     ETGLVVGFESTVDYSIALAGGVKTVFFGGEGLFLTTLTGPGRVIVQSMDVAKLASSLI
                     PYLPTQSSSSGR"
     gene            20476..21519
                     /locus_tag="PLI76_02510"
     CDS             20476..21519
                     /locus_tag="PLI76_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2117 domain-containing protein"
                     /protein_id="HOI60931.1"
                     /translation="MNLTTATMVVHGPEAFDAGDTGHLIALLAPRRVLVAGVMARTAA
                     EESGLPVTCTDERPSVVLTALSGRAFLVNRGKTPESGRIFGELVAGRLEGLVHVECSS
                     RTIYRWDGGDEGLAREIATLTGYALVRRESARARAPGTREVRGCLPGEAVFVNGIVIG
                     TATAETVVLATEGGILRPVSGLDPKPHGLEKLHRAGLPDLDAAWCKSGPVRRVPPRQG
                     GRASRGGRVAVIDHCGHTLYREIGKGVCGVLAVGDDTTAVCGHICSHAGIPVFGVIDG
                     DADAIVEPGYAPGSVVVEVIDGRDDDLGREVAATRDLQAETWDEWVEETLRTLAGRVR
                     VVVDRRGEEDAVR"
     gene            21506..22654
                     /locus_tag="PLI76_02515"
     CDS             21506..22654
                     /locus_tag="PLI76_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60932.1"
                     /translation="MQCAECKGRGLCGLERCPIMSRFYARLPVKPSDHYQGSAPSVFV
                     GSYGYPRVAGGPLMIDDADHPPDWVARGLGIDDIVGLRARTIRGAGEAKDLSGSIQEI
                     ALSSSPLDVEVRFVKPVSFDLRFDGTIAPVGLTGAIRKMNVLENARVDRAVDRATSDT
                     DLSATDACEVLNASGTDVYRITQLLTAGLLGSEKKRHVVPTRWAITAVDDTVSKQFKK
                     KIARYPPLSGIEVFSASLYGNHIVCLLVPGDWKYEMIEVWGKQSLWGGGNESIIADGE
                     GATKSGYSPIAGAYYSARLAVTEYLESVRRSARVLVLRNVTSEYWAPLGTWVVREATR
                     NAMKGPKTVCADLNEGVDLASRLIGFTHWRPHSRLIPELTTQRTLFDF"
BASE COUNT         4521 a         6975 c         6813 g         4461 t
ORIGIN      
        1 cttctcacca ttacataaaa gatttcggag gaaaaagcat caatacatat gatgtcgaac
       61 gatatagggg ttgaactgtg aagtatagag tgctggtcag cgacccgctg gcagaggaag
      121 ggattgacat cctgaaagaa ttcggtgatg tggatgtcaa taccgggctg accgaggatc
      181 agctcgttgc tgttatcgga gattacgatg ccctgctggt gcgctcgggc accgaggtta
      241 cggcgcgtgt cattgacgcg ggagcgaaac tcaagttcat cggccgtgca ggcgccgggg
      301 tcgacaatat cgataccgag gcggcgacgc gcaggggcat tgtcgtcgca aacgccccgg
      361 aaggaaatac gctcgcggct acagagcaca cgatggcgat gatgctctcg ctcgcgcgca
      421 atatccccca ggcgactgca tcgctcaaga agggcgagtg gaagcgctcc aaattcatgg
      481 gcgtcgaact gaacgacaag gtcctcgggg tcatgggatt cgggcggatc gggcgcgagg
      541 tcgcgaagcg tgcacaggcg atggagatga agtgcatcgc ctacgatccg ttcataacga
      601 aggagaaggc tgcaaccctc ggcgttgaga tggtcccgct cgatgaactc ttccggagag
      661 cagacgtcat cacggtccac acgccgctga tcaaggagac gcgccatgtc atcaacgcag
      721 agaccatcgc cacgatgaag gacggcgttc ggctgatcaa ctgcgcccgc ggtgggatca
      781 tcgacgagca ggcgctcgcg gacgggattg cgagcggcaa ggtcgccggt gcggcgctgg
      841 atgtctttga ggcggaaccg ccgacggact ccccggtcat cggcctcgaa aaggtgatca
      901 tcacgccgca cctcggggca agcacggtcg aggcgcagaa gaacgttgcg atatcagtcg
      961 caaaccagtg catcagcgtc ctttcgggcg gcgcggcgaa gtacgtggtg aacgcgccga
     1021 tgatccccgc agagcagcag gcggtcgtcg agccctacgc agccctcgcg cagaagatgg
     1081 gcagcctcct catccagctc atcgaaggaa ggcttgagtc gatcaagatc acctacgggg
     1141 gcgaggcagc aacactgccg aacacgaagt tcatcacccg cgtcatcctg aaggggatgc
     1201 tcgacccgat cctgcaggcg ccggtcaact tcgtgaacgc agaattcgtg gcaaaagagc
     1261 gtggcatccg gctgagcgag accaccaccg aagaagcgca cgggttccgg aacatgatca
     1321 gcatcaccgc aaagacggac aggatgtccg agtcggtgag cgggagcgtc tcggccccgg
     1381 atcatgcgcg gatcgtgagc atcggcggct acatgaccga cctgaccccg accggccacg
     1441 tcgtcatctc ccgccacact gataaaccgg gcgtcatcgg caaggccgcg acgatcctcg
     1501 gaagggtgaa cgtgaacatc gccgggatgc aggtcggacg gcacaagccc ggtgaagagg
     1561 cgctgatggt cctcacggtc gactccgcgg tgccggccga ggcgatggac gagattaaga
     1621 agatcgacgg catccacacg gcaaagcacg ccgagatctg atcggtccgg ccatcacctc
     1681 cttttgccgc gtgtgccggc ccccgatggg gctgagccgg cacgaaccgg ccttatgggg
     1741 gcaggaaata tatttatacc cgcgacacca accttattga tcgtcgggca gacacgggct
     1801 cgtggtctag ctggttatga cgtcgccttg acatggcgga ggtcctgagt tcgaatctca
     1861 gcgagcccat caccgttttt gaaacgattg atttcttgaa gttctttccc agaagataca
     1921 tcactcgaac acgtcagtaa catcaataat ttgtatcgat atcatacaga cgatcgtgcc
     1981 acctccggag aacgatcttc ggttcctcgc tttctcacca gggtctacac caaccatccg
     2041 ggcctgcttt ttccatagtt tcccaccatg tcgcaccgtg ccccacttcg gagcccggag
     2101 agcgaggcat gcggtctgcc ggtgaagggt tggtgtgcgt tcacgcggat gggagcgggg
     2161 aagtgcggtg gaatcggggg gtccgggtac gccggctcgc gcaccggtgg caggtgcagc
     2221 cggatcgacc gcccctgccc gtcgcaagaa ttgatgcacg actacgggag gggtttcagt
     2281 tattccgcgc tcacgcggat ggtgaaatct agtcaggcgt ttcctgatcc aaagattgtt
     2341 gccacgctgt cacaaacatc gagcgggtcg cattttcgag aacttctctc ccctcaaaaa
     2401 gttactttac cgtgatttct acgcagagat gtgccggagg atgagccgga gatgagggcg
     2461 cgtggagagg agcgcagacc ggcacggccc cactccccgg cgagcccggt ttgcacgggt
     2521 gggatcccca tcttggcgtc cagggaaggc aggggcatcc gttcgctgat gagaagaagg
     2581 gaaatccggc gatcgagcct tcgcgggacc ctccgcccag cgtgagcggg tgtacgggat
     2641 catagcccgg acaccctgcg tcaggtagag cggccgggcg ggagggtgtg atgccgtaca
     2701 cgccgatccc cggctacggg gcgcttgcgg tttgcatgga tgccgagaac aacaccttcg
     2761 gcctccggga ggagtgcacg gcttctgatt gagggggcat ctccggcgcc actctccacc
     2821 tccccggtct cttcgacgac cacccgacaa ccgccctgat gcggttaaac tgccggcagc
     2881 ccggacgacc gaagacggtc cacctgcccg atcgccggat caagagtcgt tatatctgct
     2941 ttttcatcag ttcccgaaac gttgcataac caagcgaatt ccagctcttc tttgccagcc
     3001 ggtttttgct catgacgccg agctccgcgt atgccgcacc atgctcccgg aagaaacgca
     3061 ccagcatctc ctccagcctt cttgcaaccc ccttcttccg atacataggc cggacgtatg
     3121 ctcctgcaat gtaccccacc ggtcccgcct tcgagagggg aaagaagcaa gatcgcacct
     3181 ctcctgcaat gaagccggca atgcagtctc cgtcccgtgc gatatagata atttttcgcg
     3241 ggttatcaaa gatcgatgcg atctcctctg cgatctcact tgcccgttcc gtatcgtaga
     3301 ggaagtagtc gtccccggtc tcgtctctga tctcctcgat aaggtcgatc tgcagccgga
     3361 cgagttccgg gagatcatcc cgggtgatcg tgccgaactc catcgtcatc gcatattctc
     3421 ctttgggaat ccgatagata aatacgctgt ttccctgcat catcccacat gctgacggct
     3481 gcagagggcc agggaggtcg tcaccatctg tgtcgaccta cccgagaccg gtgccgatct
     3541 cttattcgag tgcattagaa agcccgccgc cgtcggtgtc gatcagggcc gagtcgggcg
     3601 catgccagtt gccgaacccg tcgcgatacc cgttttcgaa gccgtccggc agcccgtccc
     3661 cgtcactgtc ctcgttgctc aaggtcgcgg tcaccggtac ggtctggccg tcgacgaccg
     3721 tcaccgtcgt ggtgaacggc gcgtagccgt ccttcaggac atcgagacgg tgcgcgccga
     3781 cggggatgcc cgggagggtc agctgcccgg aggccgcggt gacaccctgg tacgttttgt
     3841 cgaggggaga cttctgcgcc gcccctcaaa actgcacatt acccggaagc aagggagccc
     3901 tgcagccaga gaatccccat gacatcgtcg cctacgcgag gagcgtagcc cccggtcacg
     3961 tgcccggcgg catacagcgt gatgtcgatg tcatgatact cgccaccttc atccggcaga
     4021 cggaagagcg gaaccgtgag acggtagacg gtctttccgc agaacgaaac ggcttcgaca
     4081 ctctttaccg gcgactgata cacgaagtca tcgacatccc cctgcgggaa cgggcgatat
     4141 gcagccagat ttcgggtcga gtgctccttc ccttcagcgt ccgtgatcgt gcggggttcg
     4201 gccttatgca atacgtaggc aaaggcggcg agggcgaagg ggtgtctgcc tcccgtccgg
     4261 taacgaccga tgttccggta atatcgggtg tcgaagaaag ccagggcagc gtcgccgata
     4321 gaaccgacca cctgtccttc gagtccgttc gcccattctt cgatgcgatc taccgttact
     4381 tcattgacga cgccgtccac ggccatcggg tagccggtag agaccgatcg gtcgagggtt
     4441 atggcgcacg tcgtgaggga acgggactcc gggacgtacg cggcgacctc acgaccattc
     4501 catgcacgga tgtcggggtt tttcgcctct ttcagcgccc attccaccga ccggatgatc
     4561 tcgtccgtgg actgcgtgac acagccccag tgattcccgt ggcttcccgg ttttttgtcg
     4621 gcgccatctt ctattcggcg ccttggtgca ttcggtttca tgcgataacg cttcctcgaa
     4681 ttgtgttcct tggtgagatt ctactcaatg ctgtgaagaa tgttccgccg cagggttcgg
     4741 gtaggggggt tccgcggcgg cagttcttcg tgatatcttt cgcgatacca ctcccggagg
     4801 gtgacgatgt catcgaccag ccactgatag taggtcgtga ggacaacctc cggccagatt
     4861 tcccgtgctt cctgcagaaa ctcctcgttt gcctgaacta cggctcttgc aaatctcttc
     4921 agggggacgg tagtccgggt ataatagttc atctgcgcac tcatgtagtc gatcaaaacc
     4981 gtctcgcgat cgtccgacag ggttacggtg aactcaaaca ctgttccgga gactttcgct
     5041 tccggttcat cggaagtaaa gtgcgtcttc tcctggggaa tatgttcggg gtcgagtctg
     5101 tgaaacaccc agttgatatt gacaaggagg gagtcgaagt acgttaatgc tccgccgata
     5161 atatcgacat catcgatgaa gaagtggaag accatctcaa accccttcca gatgtctgac
     5221 cggtaaggag tggcggatac ctcccggatg cgatcctcca gttcttttct gttccagacc
     5281 atctggaccg caccgggatg gtcgatggcg atgataccgg gcatctgcct ttccggggca
     5341 ggtgtgggag atggatgatc ctgatcggcg tctaccccat acacctcgcg ataccaatct
     5401 ttgatgatat ccaggtctgc tttgaagatc cagtaaccgg catcctcacc taatgtcgaa
     5461 acgctcagga tttccacgat catctcctcc gcagagagaa taaccccgtt tgcaaatgct
     5521 ctgagcggga tttgcagggt ctggaagact ttctttccgg tatagatgta ctcaaaggtc
     5581 acgaggtcac tcgtcctgtc gtagagtata taataccgga acccttctcc cagaacgcag
     5641 ttatcgacat tatagtagcg gtgaaagggt ttgtcccgga gtcgtttcgg gtcgattgac
     5701 cggaaaacgg agaagttgtc gaggaaaaaa tgcttcaacc gggtgcagtt ccctgccggg
     5761 ttctccgcta gcccggctat gtctacactg ctcaccgata cgcaaatcga agtgcagaga
     5821 tcaatgcaat cgatataccg agggcttagg gagctgatcc tcacgacatc ctttcgaact
     5881 tcctctgtgt cccattcgat tgtaaacatc gccgccacct acaccgtcgg gttccacttc
     5941 ttgttgtacc acctgaagac ctgcggcccc ttcgatgggt agaacgtccc aacaccagtt
     6001 ttttgattga tcccgattac gatctcatca atcccgtact gatgagcatc tggagggcta
     6061 tacgcaagat tcttgggttg ttttggccac tcgctctggg ttgctcttaa tgcctcatca
     6121 atccaatcga cgatcttttc ccgcatttcc tcacgcgtca gggttgatgt gcccggatac
     6181 tgtaaagata tcccagttac tgtactccaa actaccggtt cagacgtcgg gaagagggtg
     6241 gttggatccc cgatcccttc cgttccggtg atgtgccggt tgacaatatg atccagctgc
     6301 gttttcgtga gcggtatcgc atcctgaccg accttcgtga tacgcacaac gtcggcgaga
     6361 tcaacaccac gcttggtcat ctccaacaga tggtccggag tgatctcgcc ccggaccagc
     6421 cggctcccgg ccccgtcgaa gagcaggtcc cagacctgga tcttaatcgt gtccggcaac
     6481 ggccccagga gttccttcga gagcaacacc ccgagttccc ttgccttcca cgggaacttc
     6541 cccgtatagc agacgatcgc ccccgtgatc ttgatcccgt ccccgggccc ggggatcgcg
     6601 ccagcggcgt tcatcgccgc gccgagcgga tccccgttga ggatcgcctg caccgcgtcg
     6661 cggatatctg ccatcccgcc gacgaccggc aggcaggaga acccgatcca cccgaccatg
     6721 tagtagggcg acgaagcggt ctcctccccg accagcccgt agaagagccc gtccggaagc
     6781 ccggtctccc cgaaggtcgc cccgaggatg atggcgcgcc cgatctgggc gagatcgatc
     6841 tccccgctgg cttgcggcgt cagcgggtcg gggtcggcgc cgtcgaggag gccgtcgccg
     6901 tcggtatccg ggttgaaggg atcggtgcca tactcgaatt ctgccagatc gcccagcccg
     6961 tcgtcggtgt cgggaaagtt cagatcgaca gtgtgtaggt tactgaaaca gattgaaaaa
     7021 agaagttaag ggttattctg caccgcctgc ttttcttgca agagtttcag aggatgattc
     7081 caccagagac tgtcaggaga gtacggatct tcagcgtcaa aatacgtttc aaggatttct
     7141 tcgacgtttt gcatatattt tgattttaaa gcctcatcaa gagtagagac tttttgttta
     7201 atatctgtga gatcatattc cctttccaat tctatcatgt atatttcgat ggcagtgcga
     7261 gtagatagat catttgggat ttcaagctcg taaggttcca gttcgccaaa atcgtaaatt
     7321 atcacgggat aaaaatcgac gtcgtcattc agtctatcga gtaatttttc gcggtagtcc
     7381 cgtcctcttt ttcgttccag cgtatccaat tttctggtga ttcttgcata ctcctttcga
     7441 aaaccttcgc agtcaacccc accttcttcg agcagatgca tccaaaaagg gagaaggtgt
     7501 ttgtagaaat agggtgcacc agcagagtat ctcgcatatc ttcctcccag ttcatcatag
     7561 tgctcatcga tctcccctat tcgtttttca tagtagatca ggcctctttc aatcttgtcg
     7621 ataagttttt cgtccattct ttgtcccttc gacatactag ttcaatcgaa tcagtctatc
     7681 agcgattgtc tcatcaacat agtgaggatc gatcttgggc ttgaagaacg tcgtgattag
     7741 cccatccttc gtaatactca caaactcacc taaaccgcga tcgtagacga ccagcgtatc
     7801 gtagcccatc tgataataca actcaacggt atggtctctc cgattactca aggtagtagc
     7861 tttttgcagg tatactgatt tcgtaaaggt ctcgccccat tcgaattgac cgagcacgtg
     7921 atcggtgtag tgtttgtcca gactcccaga agccgtgagc ttcttagtcg gtgcccaccc
     7981 accctcacaa acgttctcga gcatgtgcct gacgcggaag aggttgacat tcccaccgta
     8041 gagtctgacc agcgtcgcat ccgtcgtccc gtgtgccttg agcacatcga cgacatcgtc
     8101 gccataactg taccacaaga tgctcagcgc tttctcggtc tcgtccgagt accgagtcac
     8161 cccgcccagc aggagaacag ccggtttgat cagctccggg tgcttcagga caaacttccc
     8221 gacgatggcc gcaacctttg ccgcatcccc gtaccccggg atcagtgcgg cgagattcag
     8281 ccccgtcccg accaggtcgc cgttcgcaat agatgcaact cttagagact ctcctgcttt
     8341 aagtcgtcaa gcaggttgtc ctcgcttaag tcattgatgt tcggaataat ctattcaacc
     8401 gcaattagat ccacgcgacc actaatgtga acaaaatctc ctttttgtag gttgagacga
     8461 aaatagttct tatcaatatc agtaagtgtt tttccaaccc cgaagttaac aatagcgtga
     8521 tgccatcgct cagcatcttt ctgctcttct gggattgcaa tctcctcaat gcagccgtat
     8581 atgtcaagac taggttctga aggatctgga ataactcttt tatcttcggc ctcaatggtt
     8641 tccaaagatt gtaccagcat ccacaaagcc accttttgct gttttccaat catatcagcg
     8701 gtacaaagca tcttatgatc aaatagacgc gcattttgtc catttgcaaa ctcaacgaga
     8761 gtatactgag tgtaaatctt tcccatatat tccgtctccg gctcgactcc gcgtatgaat
     8821 actaaaaatt tgagtttttc tataacctct ttcctcatgt tatttcgctc caaatgggaa
     8881 tgcacttaca atatatccat tgtccccgat aacaagatgt agtatttttc cactatttgg
     8941 ctctacgtac tcgaaaagcc cgtcttcatt aaccttttgc ccgtacttcg ctccttccat
     9001 aatcaattcc ttcactgcac tatctgtgcc aaattcctga ccaaagaatt ttacaaactg
     9061 attcccccgg ggatcgagga tatgcttctc cttgatatgc ttccacccac ttggtttcac
     9121 accaagcgtt atagcctccg cttttgtaag tccttcctcc agccagacga cctttccatc
     9181 cgcacgttta acaacctcca gcgcctccaa caggttgcct ctgttttttg cgatcacatt
     9241 caggtcgctt tcggtgatcc cgtttatcag gaacttgttg accgcttcat cgccgtggat
     9301 cttccggaaa ttactcgccc attcagcggc ctcatccacg tgcggcgcga ccccgaccag
     9361 caacaccatc gcgggtttca gcagctccgg gtgcttcagg acgaacttcc cgacgacggc
     9421 cgcgaccttc gccgcatccc cgtaccccgg gatcagcgcg gcgaggttga gccccgtgcc
     9481 gaccaggtcg ccccgcgaga tcgtcgcggc gatgtcccgg aggtcgccga tgacgatgac
     9541 cccgctcgcg atccacccgc ccaggtagaa gaggttgtcg tggtcgtcga caccccactc
     9601 cccgaggacg gcgccgagca ggaactcccg gcccattacg agcgagctga accgctcctc
     9661 gtagaccagc gggtcgtagt cgggatcata ccactcctca tagtcggagt gaccgtcctc
     9721 gtcggtgtcg gccgaccaga ggtcggtgcc gatctcccac tcgagggcgt cggaaagccc
     9781 gtcgccgtcg ctgtccgggt tgagcggatc gctctcgatc gcatcctcca ggtagtcgtc
     9841 gaggccgtcg ccgtccgtgt ccgccttcgt cgggtcgctc cgctgcttgt agtaggtctt
     9901 cccgccttcg gtgaccagtt ctccagcctc gtagccgtct gaaagcccgt cgccgtcggt
     9961 atcgaccgca tacgggtcag gcgcatgcca gttaccgaag ccgtcgcggt agccgttctc
    10021 gtagatgtcc ggcagcccgt ccccgtccag gtcctccggc accaggaccg cgttcacggt
    10081 gctggtctgg cccgcgacga cagtgacata ctctgagtag gggacgaacc cctctttcgt
    10141 gacggcaatc tcgcgggttc cggaagcaac gtcggttttc gtgaattctc cggataccgg
    10201 aatggtgccg acagagaagc catcaatgga gaccgcagcc ccgggttccg gacagatcac
    10261 acggagatcg ccgcaactga aagtgggagg cagattgacg tggatctgcg tgttctggtt
    10321 ccgggtaatc tcgaccgtct cgattgccgt cccatagtat gcactcggtg tcgtcaagac
    10381 gagggtatgc tcaccctcaa ggagttccac attcgaaacc ccggaaccat agtagatgtt
    10441 gtcgatgtag acaatcgccc ccgttggggt tgacgtcacc gtcaggaagc ccgtgggtgc
    10501 gtccacccgg tggatccggg aagtggcggt ataactaaaa acatcgggct caatacggaa
    10561 catgccatta tggtctttgt aaccatcttt ctcgatcgag taagcctttg ttccgctcgt
    10621 ggggtattca gcgaaatttc tcgtcaaccc cttccgatcg cccgtttcat gtgtcttagt
    10681 taccagatct tgcgacccca tcacacccca aaaggtgacg ctcgcgttgc cgacgccctc
    10741 gccggtctga gaatctttga cccacaataa cacgtgatag atgtccgttt cccctaacga
    10801 ctcgatactt tcatcatccg caaacagcgc gtcccacctc gccgagtcca gcacagcata
    10861 cttcgccgac cccgccgcgg cgcaggagac caccccgttc acggcatcga cggtcgaagt
    10921 gacgttgacg aacgtcccga ggtcctcgtc gaagcggtag atcgagagtc gggaggggtc
    10981 cacgacgtgg gtcgggtcgt acgcgatcgc gaccgtcccg gtctcgacgt cgtcgccgaa
    11041 tccgatgttg cagaccttgc tgaccaggat ctcatcgctg atcaggtggg tgtagttcac
    11101 cgcggcgaca ctgacgttgg cgacggcata tccccggccg aagaccgtca ggtccagggt
    11161 atcgtcgagg aaccgatcgc cgctccagta gtcctcggcg ccgtccggga cctcattgtc
    11221 gttcgagtca gggatggtgg gatcggtccc gagggccacc tcggcgccgt cggcaaggcc
    11281 gtcgccgtcg gtatcggcgg cgcgggggtc ggtgccgcgc aggatctcgt ccgcgtccga
    11341 aagaccgtcc ttatcggtgt cggcaacgag cggatcggtc ccgtaggtct gctcctcgag
    11401 gttcgagagg ccgtccccgt ccgggtcgtc atccgttccg gcagcgacga tagggccgcc
    11461 catcgccgac agcatccccg gctcctccgg gaacgtgccc agcttcagca gctcgaacga
    11521 atcggtcagg ccgtcggcgt cggtatccac cacgaacggg tcggcgccga tccgcgcctt
    11581 cgcaaagacc gggaggtcgc cagcgaagac ttcgtagccg tcgatgaccc cgttcccgga
    11641 ctggttcccg gtccactgtg agcagtcgga atcggcattg agggggtcaa acccgatcgc
    11701 ctgctcaaag gcggcgggga gcatgtcccc gtcgaggagg agggtcgcgg agagcggggc
    11761 tccctgagaa tcgaccccgc tgacaacgat ggtgttcgca tccgcaaggg tcacctccgc
    11821 cgagaagttc ccgtccttga tcggaatggt cgacgagacg ccgttgtggg tgagcgtgag
    11881 actggttatg gccggatcgg agacgttccc ggcgacggtg acggtgccgc cggctcccac
    11941 cgcgtcgtcg atcggagaag tgagggtgat ggagggggcg gcggaggaag gcggatgcag
    12001 ttcgagcgag tagtactcca tggagaagtg ggtgccccag gccgggttct gcccgtgcga
    12061 gaccttcacc cgcacggtcc cggattgcgg gaaatcagcg tggaactcct ttggaaggaa
    12121 attttcagga tcagagccgg agaacccgga ttccgcatag acgacctcgt cattgacctc
    12181 gatcgtcatc cagcggccct gggggatatc ggtccccggc acacgcgcaa caagcgtcag
    12241 gctgtcccag ccgacaccgg aagggtcggt gaactcgtgc tcgacggtcc cgatcaccgc
    12301 accggcggaa agatgaacgt ccgcgccgtg ctccccgtgg ctcccgacca tgacgggacc
    12361 gtactcggaa caggagccgg tgggttgcgt ccaggacgcg gtatgcgccc agccagtcca
    12421 gccgtcggtg gaccaattcc aggccgatcc gggtgtcacg gtatggataa ccgtcacttt
    12481 tgcgatcccc cagaccgagc ccccggtcga cgtgactgcg gagatcgtag tcgtccccgc
    12541 agcccgggcc gaaaagatgc cgtcggaagt cacggtcccg accatttcgt tcgtgcttac
    12601 ccagttgaag gcgatatcct ccatcgccgc gccgtactgg tcgtaggcgg tggcggtaaa
    12661 ctggatggtc tcgccctcca ccacctcgac gatcgaaggg gtgacggcaa ccgtcttcac
    12721 aacgggaact gtctgcggtg cgccgtaggt gaacgccggt tcggtctcga cgtatttgcg
    12781 cacccggatc cagtccatcc gcacgacccg gccctcgggg gcgttgttgc cgccgaggat
    12841 ctgcatctgg tggctggaga tcccggtggc gccggtaaag acgtcggtgt atgaggcact
    12901 cccactgacg gtatgcgtct ggctggttgg ggtgatgatc caggtgctgg agtagtcacc
    12961 gccggccgaa agaccacgcg cagttcccgg tcgccaggct gccgccccgt tcatgtagac
    13021 ataccggtcc caggacggcg attccggata ccagagcaga ccgttggtgg gggtgtcgac
    13081 gaaccagggg aacgccccgc aggagtaacc ggcgttggac ggtagactat cagcatggat
    13141 gtgcatctcc acgatcaccg gctcgccgtc ggtgatcaca ggctcgttcg agaccagagc
    13201 gccagcaccc tgagcccggg tgtcatagat ccagccgtcc acaaaataat gggcgcccgg
    13261 gatcgtgtct gtccagagag tcgtatccag gtccggcccg tcgaagtcgt cgtagaggat
    13321 gtaggcgttg tcccggttgc cggtatcgat cactgcctcg ttcccgtagt acatccagat
    13381 cgaggtcgcc ccggcgggga ggttgaccca gatgacggcc gaatcggacg ccgtgtaggt
    13441 ctccttccag tagccgagga cggtgccgtc gtcggcgaca aaccgaacgt cgtcgaagtc
    13501 gctctgcata ccggaacgga acaaaacgac ctcacggtgg ggatatccgt cgactgaagg
    13561 aggggtggta attgtaaccg gcacccgggt tgcccaggcg gtgctccacc aggtcaactc
    13621 cgggcacgcg acaccccgga agatcggggc ggtgttgata tcgacccgcc cggtctcggc
    13681 gggcttggtg tagatgacct tgaacagccg ggttttcccg gcgttgagcg tctgcccgtc
    13741 gggggtaaac gggacccact ccggccagga ccgctccacc gtctcggtgc cggcgatgta
    13801 gggttcctgg gccacgaaag tttcaagagc agtgtccgcg ggaagagctt cctcaggagg
    13861 gaggggctcg ggggtagcgt ttatgggaac ttcgtcctca aagggaacgt tttccagggc
    13921 aacagtttca ggtataacgt cttcataggc aacattctca gaaacaacgt tcccagggat
    13981 aatatcccgg tattcaatga tggggatctc gatgctatgc gatcgctcct cccagacgaa
    14041 ccactcggcc cgggtgatgt tctcgttccc gttctggagc gaccatgtgg ccaggaagtc
    14101 ctccgacccg tcgaggatca tcgtacggtg ggcggtgatc tcgaagatct cggtgaaggt
    14161 gccgagatcc ggggtggcgt tcaccaaccg gatctcgaga tcggcatcgg cggcagatct
    14221 gctctcatcc gtgatgcaga tggtccgggt ctccggctca taggttttga gccggatctt
    14281 gccggaatcg gtgaccacgt aagtggcatt tgatgctaaa gaggcatcag tacctgatcc
    14341 tccagtatcc cgaatttctg cagaagtgga gatagaagtg ttggtccatg acgtgggttg
    14401 ggaccagttc ttttccgacc acaatgatgg attttcctga gattgaacag tattgaatgc
    14461 tgctttgtgc gtatccaagc cagaagatgt cttgtcgggg aacgagtctc cggcagtatc
    14521 catgttcaat gtagttgcat tcgattccgc ggcgctcacg gcaggaatga acgccgtggt
    14581 tatcatcaac accgccatga gaagagaaat aactctctcc atacctttcg tgctcatcgg
    14641 taatcaccct ttgaaccgga ggcacaacga gcagactctt aagtatgaag aaaatccacg
    14701 cgttctgtct acgggctgtt gtggtgttca tccatctgtc gaggacagga tgaataccac
    14761 tcacatctcc ctgaattgat caagcattgg acggctcaca aactctcatg tgacaattcg
    14821 tcctaagagt aactttcact ctatcaaata cataaatgca tgtggtttga taaaaaacaa
    14881 aacatggtga attaatacat ttttattaat aatttgacga caaaatcatg aatttttacc
    14941 gtaccgtaag catttgtgga taattgcttt atgtggtcgt tgccttattt acacgggatg
    15001 cagaatccgt atcgacgggg tcgcgctgcc gtcccccggc atcggcgacc tctgcatcag
    15061 ttacaggaca ccgggcctga gggtagatgt cggagagtac ggcagttact ccatcatatt
    15121 ctcaaagggc gacgtttacg agatgttcat cactgtcggc ccctcttcgg actacgaact
    15181 cctgaaggac cttgccgaac gcgccgcggc aaagctcccg cagtaacggc tgcaggctcc
    15241 ccaccatttt tgttcatcca cgccgcaccc ttgattcccc gagcacgtac ccggcatacg
    15301 gcaccctcga caccgcccgc accaccgcgt aactcaccac ggccgccacc acaaagatca
    15361 ccgggtaggt gagcccgtcc gcccacgaga gcccggtgag ggagatccag agagtcgcgg
    15421 cagcggcgat gacgagcggg tggatgaggt agaccccgta ggagtggtcc ccgaaggacc
    15481 gggcggcgcc cgccatctcc ccaccgcccc catccagcag ccaggccgcg aggacgagga
    15541 cggcgatgac cgggacgtag tagagcggct cgaggatccg atagatgcag aagaccgcaa
    15601 gggacgtgcc ggcgaggctc ccggagaggg cgacggccgc cacccagacc ccgccgatga
    15661 ggagcgcccc cccgccggcc gcggcgagga cccacgccgg cgagagcgat cggagcgtcg
    15721 accggaaccg gtccgtgtgc cgtccgacgt ggatcccgag gacgaagtag aaaaggtgcg
    15781 aggggaagag ccggatcagg accgtatacc actcagggcc cacatatgcc cccatgaggt
    15841 gggcgcctgt gttccagagg gccaggagga agaggagcga gagaaggagg tagagcgccg
    15901 ccccgaaccg gtcgaagagg ccgtagcccc ggacgatcag cgggaagagg aggtagagct
    15961 ggatgatcag cgcaaagaac cagagatggt aggcaccggt cccgaggagg agggcttccg
    16021 ccacccgctc cggagagggg atgccggcaa acccgatcgt cccctcgacc gcgatgagga
    16081 gatacagggc cgtgaagaag aggtagggga tgaggatcgt ctttgcccgg cgttggtaga
    16141 actcgcggat cgagaactcg cccgtatacc gcgccgcgag cacccacccg gatatgaaga
    16201 tgaagaccgg gaccgcaaag tgggcggcga tgtagatgaa gacgttcgtg agcgcaagca
    16261 ggttcacgtc ggggatccgg gtgtagttca tcgagacgtg gacggcgatc acggcgaggg
    16321 cggcaacccc ccgcatgtaa tcgacctccc ggaaccagac caccagaacc acccatcacg
    16381 ggcccgcccg gacaggcgag cagtacaaat tgcaggccgg tgtatataga gctcctgccc
    16441 ggcggccggt caggcactat cattatccgg gaacaccatc tatcttgaga gttgagtcca
    16501 tgcagtactc ggaagggcag gtcggcaggg tcttctccgt ccggatcgac gacggagagg
    16561 acttcatcag ggagatcgag cgatttgtag cggcgatgaa catccagagc gggatggtcc
    16621 acttcctcgg cgccgtccgg aacgcgacgc tggtgacggg gccgaaagat gcagtcatcc
    16681 ccccgacgcc gcgggccgag gagatcttcg ggggctggga actcttcggg gttgccacca
    16741 tctatcccgg cgtcgagggg ccgtcgctcc acatccacag tgcggcaggg aagggaatcc
    16801 ggtcgctcac cgggtgcctc cgggagaagg cggaggtcta cctggtcata gaagcggtcg
    16861 tcatggagtt cgtcggcata tccgcccgga ggctcccgga cgaggcgacc ggcgtcaacc
    16921 tgccggtctt tgaccgggta ctcccatgag cggtcagccc gggttcctcc ccatgacgat
    16981 ggcggaggcg gaacggctcg gtatcgaccg gttcgacatc atcctcgtct ccggtgacgc
    17041 ctacgtcgac cacccctcct tcgggaccgc gatcctcggc cgggtcctcc aggacgccgg
    17101 ttactcggtc ggcgtcatcg cccagccgga ctggagaagc gacgccgact tccgccgcct
    17161 cggcgagccc cggctcttct tctcggtctc ggccggaaac gtcgactccc tggtgaacgc
    17221 cttcaccccg aacctgaagc gccggagctc ggacgcctac tcgccggggg gaagactcct
    17281 ccggcccgac cgggcgaccc tggtctacac cgaccgggtc catgccctct tcccgaagac
    17341 tccggtcgtc atcggcggga tcgaggcgag cctccggcga ttcgcccact acgactactg
    17401 gtccgatacg gtccggcagg ccatcctcgc cgacgctccc gccgacctcc tggtcttcgg
    17461 gatgggcgag caccaggtga tcgcgatcgc ggaccgcctc gccgccggtg agccggtcgg
    17521 gtcactcacc gatatccccg gcaccgccta ccgggtggac ctgaagacct ggcggggcat
    17581 ggacccggcc ggctacgtcg tcctcccgga ctacgccgag gtgaagagcg accgctacgc
    17641 ctatgcaaga gcgttcgcgc tccattataa cgagcaggac ccattccggg gccggcccgt
    17701 ggcgcagccg cacccgaaga ccgtcgtcat ccagaacccg ccggcgatgc cgctctcagg
    17761 agttgagctc gaccgggtct acgaactccc ctacacgcgg agggcgcacc cctcctacac
    17821 cgagcccgtc ccggctctcg agccggtcag gttctcggtc gtcagccacc gggggtgctt
    17881 cggggcctgc tcgttctgcg ccctcaccca ccaccagggg cggatcatcc agagccggag
    17941 cgtggactcg atcgtccggg aggtggagcg gatggcgaag atgccggagt tttcgggcgt
    18001 cgtccaggac gtcggcggac cgacggcgaa catgtacggg atgcactgcg gccggtggga
    18061 gaccgccggc acctgccccg accgccgctg catcgactgc cccacgctcg accgcagcca
    18121 cactgagcag ctccggctgc tcgaacgcct ctgcgagatc ccgggggtga agcacgtctt
    18181 catcgcttcg ggcatccgct atgacttaat cggtcccgag gaccccgact accttgccgc
    18241 ggtctctgcc caccacgtct ccggccacct caaggtcgct cccgagcacg tcgcagagcg
    18301 tgtctgtgcc cgcatgggaa aacccccccg cgagaccttt gataggttca gggagcagtt
    18361 cgaggccctc gagaagggca agaagaagcg gcagtacctc gtcccctact tcatgtccgg
    18421 ccatcccggg tgccggatgg aggatatggt cgaacttgcc gagtacgtcc gggataccgg
    18481 gctctacacc gagcaggtcc aggacttcac cccgacgccg atgagcatat cgacgacgat
    18541 ctaccacacg gggctcgacc ccttcaccct tgaggaggtc tacgtcccga aggggcggga
    18601 gaaacgggtc cagcgggccc tcatgcacta ccgggacccg gagaactacg cgctcgtctg
    18661 cgaggggctc cgggcggccg ggagagagga cctcatcggg aacgcgtggc ggtgcctcgt
    18721 cccggcccgc cccgggggcg gcaggcttac ccgttcagga tcgagccggc gacaaacccg
    18781 gtagccaccg cgaggggcct gaacggtatc cgggggatca cgagcgtcgc caccgcgagg
    18841 gtggccgcga agttggccgt cgttcccggg gtgacgacgg catcgagcgt ccgcatcgtc
    18901 gaggagcgcc atccgtcggg aaggggcggc ggagagagtg cgaacctctc cttcacgtcg
    18961 ctcaccgtct tcatagtcgt cgaccgccac tcaccgactt ccgatacacg ccgtctgcag
    19021 atcataatca ctggtgtggc acggagatca cttatggtta tggctggcgg gccggaaccc
    19081 ggcgcatgcg gttacggtgg cagaaggggc agagggcctc gagccttccc ggcacgtgag
    19141 agccgccgga gaccgcatcc tcacgaggtc gaagcgcgtc gacccggcgg tgagggggat
    19201 gttccggcgc accggcgaga agaatacgga gcgtctaccc aactaaagcc cccgggatgg
    19261 cgcaccctcc tcaagcatcc tgttcacgag atcctttccc gagtccattg cccccatcag
    19321 gcgttcaacg tactcgcctc ccacctcaag atacccgagc acgtcacgga cgagcgagag
    19381 caggtcgtcg gcgacctgca gcagccattc gacaaaaccc cacatcgact cggccgtccc
    19441 gagcacggcc tccggcgcgt cgcggtcggg cagcgagacg ttgatcacct ccgtgcccgc
    19501 gaaccctgcg acggcaatga gaagcagaac caaaatgatc ggtctcatac cgccaccctg
    19561 ccggccggga cggtaccggg gcgtatgccc ggggcaaggg ggatgataaa cgatccattc
    19621 tccatacaaa tcgttacggc atcaacccca attaaggcca ccgctccgga gggggttttg
    19681 gaaacacaac cgccggcccg gagaaaacag agagaaaaga cctattgccc aaaaatgaaa
    19741 gaagttccgg tatgcagtac gagatcaccg gagacaacct gcagatcgtg accctgcgcc
    19801 tcgcccccgg cgagagcatc tgcgccgagg ccggcgccat ggtcaacatg agcgggaaca
    19861 tgcagatgac caccaacatg aagggcgggc tcttcaaggg gttgaagagg atggtgaccg
    19921 gcgaggggct cttcatgacc gagttcaccc cgcagggagg agagggcttc gtctcgtttg
    19981 ccgggaacgt cccgggcaag atcttcaccc tcaaccttgc cgggaacgag ttcatcgccc
    20041 agaaagatgc cttcctctgc tccgagcagg ggatcgacct cgatatcgcg ttcacgaagc
    20101 gcctccggtc gggcgccttc ggcggcgagg ggttcattct ccagcgcctc tcaggcagcg
    20161 ggaccgcgtt cctccactgc tgcggcgaca tcatggagat gacgcttgcg cccggcgagg
    20221 tcgtcaaggt ggagaccggg ctcgtcgtcg ggttcgagag caccgtcgac tacagcatcg
    20281 cgctcgccgg cggtgtgaag accgtcttct tcggcggcga agggctcttc ctgaccacgt
    20341 tgaccggccc gggccgggtc atcgtgcagt cgatggatgt cgcaaaactc gcctcctccc
    20401 tgatacccta cctccccacc cagagcagtt catcgggacg gtaacctgat agtccaggga
    20461 gctcaaattt acggcatgaa cctcacgacg gccaccatgg tggtgcacgg ccccgaggcc
    20521 ttcgatgccg gcgataccgg gcacctgatc gccctcctcg cgccccggcg ggtgctcgtc
    20581 gccggggtga tggcccggac tgccgccgag gagtcggggc ttccggtgac ctgcacggac
    20641 gagcggccga gcgtggtcct caccgccctc tctgggcggg cctttttggt caaccgggga
    20701 aagaccccgg aatcggggag gatattcggt gaactcgttg ccgggaggct cgaagggctc
    20761 gtccacgtgg agtgctcaag ccgcaccatc taccgctggg acggcggcga cgagggcctt
    20821 gcacgcgaga tcgcgaccct gaccgggtat gccctcgtcc gccgtgagag cgctcgcgcc
    20881 cgggcgccgg gcacccggga ggtccgcggc tgcctccccg gcgaggcggt cttcgtgaac
    20941 gggatcgtca tcgggaccgc gacggcggag acggtcgtcc tcgcgaccga gggcggaatc
    21001 ctccgtcccg tctccggcct cgaccccaaa ccccacgggc tcgagaagct ccaccgtgcg
    21061 ggcctccctg acctcgatgc ggcatggtgc aagagcgggc cggtgcggcg ggtcccgccc
    21121 cggcagggcg ggcgcgcctc ccgcgggggc agggtcgcgg tcatcgacca ctgcggccac
    21181 accctctacc gtgagatcgg gaagggggtc tgcggcgtcc tcgccgtcgg cgacgacacc
    21241 accgccgtct gcggccacat atgctcccac gcgggcatcc ccgtcttcgg ggtgatcgac
    21301 ggggacgccg acgccatcgt ggagcccggg tatgcccccg ggtcggtggt ggtcgaggtg
    21361 atcgacgggc gggacgacga cctcggccgg gaggttgcag cgacgcggga cctccaggcg
    21421 gagacctggg atgagtgggt ggaagagacg ctccgcaccc ttgcggggag ggtgcgggtt
    21481 gtcgtcgacc gccggggaga agaggatgca gtgcgctgag tgtaagggga gggggctctg
    21541 cgggctcgaa cgctgcccga tcatgagccg gttctatgcg aggctcccgg tcaaaccgag
    21601 cgaccactac caggggtccg ccccgtcggt cttcgtcggg agttacggct acccgagggt
    21661 cgcgggaggt ccgttgatga tcgacgatgc cgaccacccg ccggactggg tcgcccgggg
    21721 cctcgggatc gatgatatcg tggggctccg ggcccggacg atccgcggcg ccggcgaggc
    21781 aaaggacctc tccgggagca tccaggagat cgcgctctcg agcagcccgc tcgatgtcga
    21841 ggtccggttt gtgaagccgg tctccttcga cctccggttc gacgggacga tcgcccccgt
    21901 cgggctcact ggcgccatcc ggaagatgaa cgtcctcgag aacgcccggg tggacagggc
    21961 ggtcgaccgg gcgacctccg atactgacct ctccgccacc gatgcctgcg aggtcctcaa
    22021 cgcctcaggc accgatgtct accggatcac ccagctcctg accgccgggc tcctcgggag
    22081 cgagaagaag cggcatgtcg tccccacccg gtgggcgatc acggcggtcg atgacacggt
    22141 ctcaaaacag ttcaagaaga agatcgcccg ctaccccccg ctctccggga tagaggtctt
    22201 ctctgcctcg ctgtatggga accatatcgt ctgcctcctg gtccccgggg actggaagta
    22261 cgagatgatc gaggtctggg ggaagcagtc gctctggggc ggcggaaacg agtcgatcat
    22321 cgcggacggc gaaggcgcga cgaagtccgg ctactcgccg atagcagggg cctactactc
    22381 ggcacgtctc gcggtcacgg agtacctgga gagcgtgcgg cggtcggcgc gggtgctcgt
    22441 gctccgaaac gtcacgagcg agtactgggc gccgctcggc acctgggtgg tccgggaggc
    22501 gacgcgaaac gccatgaagg gtccaaagac cgtctgcgcg gacctcaacg agggggttga
    22561 cctggcctcc cgtctcatcg ggtttacgca ctggcgcccc cacagccggc tcatcccgga
    22621 actcacgacg cagaggacgc tttttgactt ttaaagcgaa ttttgcccat tcttctgcag
    22681 acggtagcgc tcgatgaggg agtcgagcgt ctcggcctcg tcgacgctct tgtcctcgcg
    22741 cacccggacg aaccgcggga accggagcgc
//
LOCUS       DAOSEE010000018        22452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1010_length_22452_cov_243.324954, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000018 DAOSEE010000000
VERSION     DAOSEE010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 22452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22452
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1010_length_22452_cov_243.324954"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..829
                     /locus_tag="PLI76_02520"
     CDS             176..829
                     /locus_tag="PLI76_02520"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOI60933.1"
                     /translation="MRERIKETDQGIQGEVDVRNFDRMQRHLRDRGLIATDEIIRSGI
                     AGGLALELGPGPGYLGLEWLQRTDETSLKGVEISRHMIDVARKNAREYGLAARVEYTL
                     GRVEAIPFADAAFDAVFSNGSLHEWSKPERAFHEIHRILKPGGRWYITDLKRDMNPAV
                     IWFMKVSVRPAEMRPGLQTSIDAAYTRSELETILAGTPLESSSLSENPFGLRVLGVK"
     gene            complement(859..1089)
                     /locus_tag="PLI76_02525"
     CDS             complement(859..1089)
                     /locus_tag="PLI76_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60934.1"
                     /translation="MAEERRRRRDEQPDEEIPFEDKGTDIVYSPVPAELGNEGRDPGR
                     PPGAPKTPTPRAEHERSKKGEERIAREQGGGE"
     gene            complement(1141..2343)
                     /locus_tag="PLI76_02530"
     CDS             complement(1141..2343)
                     /locus_tag="PLI76_02530"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOI60935.1"
                     /translation="MVKQFKELSEKEGMGENVTMRRTALMIVLAAVLAPMVAGGGVSM
                     GSAVNDVAISADGRYAVAGTDGGMIACLRQDGSVAWDTSAGDPVTAVAVADARGYVAA
                     GTNAGDVILLDGASGNRYWTERVTGPVRDLAFSGDGRSLAVGSSRVTLFDHQGKEGWN
                     FSTGAGATVSSIAFTADGNYIAAGSDNGVIHLIGSRGNLLWQGIAYGPVTAVDASRDG
                     TTVAAGGRDRVLQVYGRSGGLPWVVQAGAPILALALPADGRFVIIGKQGGDLDCYRPR
                     EGLIWQNHTESGVLAVDVSEQGEAVAAGTAGGTLRLLSGDGGVLWSFDAGAPVTSVAL
                     SANGGYIAAGAGERVFVFRTADVPVEATTTPAEKATPAATPTQAAGAGALIGAGAVAV
                     AGGWLRRR"
     gene            2357..2869
                     /locus_tag="PLI76_02535"
     CDS             2357..2869
                     /locus_tag="PLI76_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HOI60936.1"
                     /translation="MKAAGEQPIPRVSITIKRAEEAVARFMQGYNCAQAVSSVFSHDA
                     GVPEETILRAATGFGGGMGRTGGACGAVSGAVLVIGLMVGSAGPDEKEAKDLTYDLTR
                     EFVEQFAERTGSVSCTELLGCDLSTDEGRARARRDNLTRTLCPRYIRNAVDILEEVLA
                     PVISGRPASR"
     gene            complement(2841..3947)
                     /locus_tag="PLI76_02540"
     CDS             complement(2841..3947)
                     /locus_tag="PLI76_02540"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOI60937.1"
                     /translation="MNTERPGAAGGGGSVPGGRFSNNFDFLRFAAAVAIVILHAYALR
                     LGYTGIGLSNPFVLMGQAGLAVLLVTSGYLIAASWESTASPLRFAWKRFLRVVPALIP
                     AVLITLFVIGPLMTTLSPGEYFNALFSPAGLATIPFFEDGSAIGLFQENPWTYVNGSL
                     WTIPVEVAMYGVVALLGIAGLLRRWGAIPALAVVNILVWIYWFDDPRMAKVRFTLYFL
                     IGAYLYLHRERITYRPAVAGALLLLLGLSTLTPYPTVAGVVCIPYLTIYAAHLPIPYL
                     NTFGRSGDFSYGIYIYHYPVQQTLILVTANTLLLPALFGLSFAVTFALAFLSWNLVEK
                     RALTAKNLGTGEIARMLRLPSLPGPLTGWRAARK"
     gene            4068..4853
                     /locus_tag="PLI76_02545"
     CDS             4068..4853
                     /locus_tag="PLI76_02545"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family glutamic-type intramembrane
                     protease"
                     /protein_id="HOI60938.1"
                     /translation="MDPLVVATFAVVILLEIIVPLALGYWIVRRFGVPWRIFLFGALF
                     FIAVQVVHTPLVLVTQGPLYLALLPSGTTMALAGLAVYLGLMAGLFEEIGRYVVYRYY
                     FRRRGIPLTRETGLQFGAGWGGVESIFVALLVLSSMVSYIALTSDGGMVPLPDDPAVQ
                     AQVETLKALTPLDILPGLAERMMTITLHVAWSLLVLASLVYGERLLLVLAVLWHAAVD
                     AAAVYLAATMGILVTEAVVFVFAVIGLIYILWEWRRMGARAAS"
     gene            complement(4850..5716)
                     /gene="thiL"
                     /locus_tag="PLI76_02550"
     CDS             complement(4850..5716)
                     /gene="thiL"
                     /locus_tag="PLI76_02550"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOI60939.1"
                     /translation="MDERGLHRLIGTIIDPERLSDDCAVIPCGNLLMVASTDMLHETT
                     DFPAGMTDWQVGWMAVAVTLSDIASMGAVPGQVLLAVGLDRPERLAGIMTGARDCCTA
                     FGAELVGGDLDAHRELTIVSTGLGTVRPEHIARRRGARPGDVITVTGPLGEAEAALAG
                     YEQHRKELLEPQPRVAEGESLGRAGVSAMMDISDGLALSLYDLLAVNDCGFAIETARL
                     PLPAGVPEQEGRELALHGGGDFELLFTAPADILPVPGVRTHVIGRVIPERVVLADGKP
                     LERRGYLHEWGG"
     gene            complement(5709..7658)
                     /locus_tag="PLI76_02555"
     CDS             complement(5709..7658)
                     /locus_tag="PLI76_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF460 domain-containing protein"
                     /protein_id="HOI60940.1"
                     /translation="MKVFGIDIIKGSVRSRTRRPVYALARAEDGEITGVEEVTGFRLQ
                     RILAAEEPDILAVDSIQEIAADQHELRTFLQALPPSTKLVQVTGGERTESLGKVAARY
                     NISFNKFDPYAEARTTAQVASLGAGVEVIAFENTTDIVVSRHRSPGRGGWSQNRYARK
                     IHGAVMQKAREIEARLRGAGLDYEKKETKAFGGFSRVLFRVVAPRDRVPVHSSRGADV
                     QVRVAGRELDRIRFEPRSSRPRYLIVGLDPGTTTGIAAVDLDGNLVLLTSSRQMTMSD
                     IVEELYGAGKPLIIASDVREMPYSVEKIRRAFNAIPYTPKQSLSVEAKYDLTARFSYT
                     NDHERDALSAALEAFRSLQNKFRNIVKRVRPGVDLDEVRARVLRGQPLDAVLTDLQGA
                     PAAPREAEPAAAVQERPIEDERVMALDGMVKRLRSYVQELQEDLRERDREVERLQQDL
                     RRARSATERKVRRDAELATKDATIESLREQLRGERRRSRRLKKRLERMQTVAELEVSE
                     DYTPLKVLDSLTRDGVRALQEEIGIAAGDVLYVPRAHGWGRGVVKDLAGMGVRALVVG
                     GEPPDPHLLRVAREAGLPLLPAGAIEPDIRGRTGAAKTGAVSEALGEWEEGQKEYRRE
                     QEAERVEYLFKEYRSEREKEVRRGG"
     gene            complement(7655..8512)
                     /locus_tag="PLI76_02560"
     CDS             complement(7655..8512)
                     /locus_tag="PLI76_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HOI60941.1"
                     /translation="MPVPAEYVRSLHAYELRILLALERLMRRYQWVPLEVLRPATGFS
                     ESELAYRLGRLMAMGMVRYEKTPYEGYALVFDGYDTLALHTLTRRGTVQALGCLIGVG
                     KESEVYEAMGLGVVVLKFHRVGQRSFQSARLKRGYMPEAGHCPWLFASSHSAKMEYDA
                     LKTLHPAVSVPLPIDQNRHVVAMSFVPGVNLSRAALAEPEIILDEILDNIREAYRLGI
                     IHGDLSEFNVMVDEKQCWLIDWPQWVEIAHPNAGEILARDIGNILQYFKRKYGIEYAF
                     EEALARVVE"
     gene            8562..9455
                     /locus_tag="PLI76_02565"
     CDS             8562..9455
                     /locus_tag="PLI76_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00269 family protein"
                     /protein_id="HOI60942.1"
                     /translation="MQCSKCRRDAVIYQRYSGLHLCEQHFNRDLEAKAKRAIREHRWI
                     ASGDTVAVALSGGKDSSAVLYFLHRLLHERRDVRLMAITVDEGIGGYRDPARARAIAE
                     RIGVPWVTASFREEYGVTLDEIVARKGTGLSCSYCGVLRRALLNRVAREEGVTKFAYG
                     FNLDDEAQSVLMNVLRGDPYRLTRPVREVAGFVPRIKPFMYIPEREVALYAFFHVEGF
                     DLAGCPYAADALRGDVRGILDAYTYRHPATKYSLVNLGEALRNPEQPPEGGLRVCERC
                     GEPCGEVCRACQVLDEVRRGA"
     gene            complement(9463..10185)
                     /locus_tag="PLI76_02570"
     CDS             complement(9463..10185)
                     /locus_tag="PLI76_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase family protein"
                     /protein_id="HOI60943.1"
                     /translation="MNGYWDGPKPEPQARTIDEMREVLADPGCTFDQPLYFMYRDLAK
                     NESDRAWLRQQHIRYDITVIPAATLCGEHVKTMGHYHTLNPAGVFYPEIYEVISGTGH
                     YLLQTRSADDVVMIRGIAGDKILIPPGYGHVTINPGKVDLVMANLVSAEFSSNYGPFA
                     EKRGAAYYETEGGALVKNPHYPDASPVRYCDPTEVPELGIEKGTGLYDLIGRPRSVAF
                     LNHPEQFMEIFAGVTGGCLPML"
     gene            complement(10185..11462)
                     /locus_tag="PLI76_02575"
     CDS             complement(10185..11462)
                     /locus_tag="PLI76_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HOI60944.1"
                     /translation="MRWQHVPVLMLLLALLAVPGSAAGAKYLYGNPELSATIAGTNEF
                     APGAETGLTVIISNSGLNTHKIAIPSLPPDDLPNTAKLVTVALKSNDAPFTVKTDPQF
                     LSDIMGGAALPATFNVKVADSAAPGTYTLPLEVTYTYLQDAEDYGDLLRYNYQKKTET
                     LPLTVLVTPGLRVEVLNVQTRSLNVGTEGYVSMTLKNIGHDAADKAVAKVARNGASPL
                     IPTDSNAYIGTFAPGETVDVTFKVAVADSAEPQSYPLDIVVAYEDYEGKSTASRPVTI
                     GLPVGGKIDFDVASPTVTLHPGGKSILEVVYRNAGAAKVYSAQARISAVDPFTSSDDT
                     AYLGDLAPGETATARFEVSIDADATLKNYGIDSEIRYRDDLDNSKISDTMKVQVTLEK
                     KEGTLFTNPVFLAVIAVVIIGAGYYIFAYRKKK"
     gene            11526..12317
                     /locus_tag="PLI76_02580"
     CDS             11526..12317
                     /locus_tag="PLI76_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOI60945.1"
                     /translation="MTAPRDIPNDLIESLKSLGLTRYEALVYIGLLRTTGATATEIHE
                     TSGVPRASVYPVLDRLVQKELVSVSHTTPKRFDAVPPGQGIENLMRRIEDDAESARSA
                     LERLYREKRPEARGNQEPIWTIYGEENIRARLSDMFLGAERRVEMLVARDLLVEHVLA
                     LLGPVPESVQVDIITDTWEGEQPSRFRIHAHPPGAHQKPHTSPGKGLRYESSGIFLVD
                     DVRALVWMGSSGEQPSALYSESTGLVQFVRRYLTSIMEWVGATGQ"
     gene            complement(12311..13057)
                     /locus_tag="PLI76_02585"
     CDS             complement(12311..13057)
                     /locus_tag="PLI76_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF128 domain-containing protein"
                     /protein_id="HOI60946.1"
                     /translation="MHVPLKFTNHRIEEYALRVTYRPEEDTGTIIYNLSLIENRDLEF
                     ALSVMKEAHRTGITISDRIRVAEAGEEIGGYTVPDGRHAVCTMCSITLDALLLRRGIP
                     LNPIGGGVVEIEARVPRRFTSMILYRDTTLDPLEVLISQETTSILDVMHHGNGSILAN
                     IRECHMEAEPLVGTVLDELAAIGFSGVLDVGAPNAPLLGVPVSPQYVGVAMVGGTNAM
                     AAVREAGKPIVTRALKGVIDIREMGYLEDY"
     gene            13302..14267
                     /locus_tag="PLI76_02590"
     CDS             13302..14267
                     /locus_tag="PLI76_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60947.1"
                     /translation="MESGTREKARNVLKRILEAASFAVAEVDDPLVLSGIRGDTALLV
                     LCSDDPETIRKFDSMNYRLRTGDDDLVCRKLLFTLNPAVQTEDCMRWGADEFTLYAGR
                     AALAKVLGQTLVLDIGRPPPATRGESVEDAPVAVEEEPEAGPELPHLPVRITEKRACG
                     IAGLHGSAKCRFIPCWNYRYVSTGEREVKDRLVSFDAEGAGSINAINGMKMEIDQGSI
                     EREPVPFGSEIVPARLVREDVEGQIVREVIDRLTQRLRFRYEKGDAIFYEEKTLKPDR
                     SNIKVDLEIIYVPVWQVRGGSRIVEVNAFTGEVLSMPMDEGVELL"
     gene            14274..15599
                     /locus_tag="PLI76_02595"
     CDS             14274..15599
                     /locus_tag="PLI76_02595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOI60948.1"
                     /translation="MIVVIGGGPAGRIGAMHLAHAGREVRLIEKRKIGGQCLHDRCMT
                     ICALNDIARLIEYARTQKDLGILDSVPTVSYPAVVRRMRDVQEKLSTVLDVETRRAGV
                     DIVYGAEGRLEGDRVFVDDEEVRADAVLAATGSRAAIPDIPGTDLAGVYTYQTLPDME
                     ELPCRMVIIGGGVVAAEFAHVFRAFGSNVDIIARHEVLRGLDGKVRAAAIRDLSGVEI
                     REETAVLRIEGSDRVRSVLLAGGEEIEADAVLLATGLLPRSESLQGLKKGPDGAVIVD
                     RRMRTSVEGVYAAGDVIGPPYLTPAARREGTVAAENILGHPAIMDYTGIPQAMSLRYD
                     YAFATTGADGEGVTLSAPAPAGPGSFWDVAGGWAGLAQVRIDPETGRLIGAAACVPGA
                     ATLLSYVGYLMKRGITASDFDDIVEVHPSTDGVYGLARYAADMLRKRDG"
     gene            complement(15615..17237)
                     /locus_tag="PLI76_02600"
     CDS             complement(15615..17237)
                     /locus_tag="PLI76_02600"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HOI60949.1"
                     /translation="MGPHTIVIIGHRQPDTDSICSVIGYAELRNHAEPGKYIPARCGE
                     VNAETRFALETFNVEPPVYIASVEPSVSDIPLDTRSARQDVPTVDVAALMDTYDMRNI
                     PITDDEETLVGLVSEYGLARAYVRKNPREQLSLVPMPLATLARILDARTVVSAHEVLG
                     EGRVYTVIDALHVTLSRMTPDDIAIVGDNEPAQLALISAGVAALVIAEGAPVGERVIA
                     AARARGASVLATTLDAFSVGKMISLSLPARMIMETDVPTVRLEDSLEYAKGVVSSSRF
                     RTACVVGEDRRHIGLVSRTALMQDVQKSVILLDHNEYSQAVDGIEQADILEIIDHHRL
                     GAISTLKPVKFLNDPLGSTSTIVANKFIEASIEPTPPTAGLLLAGILSDTMVMKLSTT
                     TPVDIRAADYLAEIAGVDPAEFGPKLIQKGMDLDALPKDELLTRDMKRYNLFGRSIMV
                     SQVMTSSFEFAESEAGEIRTELDRLRTTMGVDCFFALFTNVFGDASDLFAAGDEACIA
                     KLGLRDQPVRLKGVMSRKKDFIPKFGQMFRQI"
     assembly_gap    17440..17539
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            17609..18283
                     /locus_tag="PLI76_02605"
     CDS             17609..18283
                     /locus_tag="PLI76_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI60950.1"
                     /translation="MSDEPLLRFEAVSKVYPLPAGDVVALDRVSLTVDAGEFIAVMGP
                     SGSGKSTLLHLMGCLDVPTGGKISLSGQDISGMSDDDLTGLRRDRIGFIFQQFNLIPL
                     LSAVENVEFPLTLTIGREESRRRAIEVMRAVHLDDALFTHRPAELSGGEQQRVAIARA
                     LVNNPDLLLCDEPTGNLDRKTGTAIMEILEAENREGKTVIVVTHDPEVATYARRTIRI
                     VDGKVV"
     gene            18280..18516
                     /locus_tag="PLI76_02610"
     CDS             18280..18516
                     /locus_tag="PLI76_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60951.1"
                     /translation="MIFLSFALRSPIAPPGHRDHRRRYGAFTFGEMFAAFNTTAIGEI
                     SFAVALGIGASLTDGLSPSWKAAQLNPIEVLRYG"
     gene            18763..19932
                     /locus_tag="PLI76_02615"
     CDS             18763..19932
                     /locus_tag="PLI76_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60952.1"
                     /translation="MITWLNKNFSSLQPTRAIVMRALRHLRPADRKKLFSEDIPEMRT
                     AEGRWFEAIVYEMILDLSLKTDLILAVVARGADGPSKVRRAQLGQNGLFYSNIGDIKV
                     RGNGQDLAEVDLMLVDHTGALTFGEIITSPADLKEFEAEIRYKKQLLGYLYGQPTVPF
                     LLISSVDISRTAVVRRLLKEPDNILLTTPTCEDLKSLIRPRDLKRSPPRQIRHEKLIS
                     IQDIAPRRPFDYKELHDERLQSIVAAVTSQKGIEKLGTPDEIPPIVKKVLFGGLYPSA
                     VRMLDARYPIQIKGKTYDPDAIQKQFSKVVLAVNIPEYRPIIYLRTRDKKEYLKMVPS
                     REGGFKFESRRTPHMAGFFLWLESIRTSLGAELTQELLDAFPAVHVSPAPAAGGS"
     gene            19991..20647
                     /locus_tag="PLI76_02620"
     CDS             19991..20647
                     /locus_tag="PLI76_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOI60953.1"
                     /translation="MDLIAELVSFLGFGLVAIASITAVMNPASTIAVYTALTEGMGGS
                     ERMKVIQTSMKIAFIVLAFFALTGQLIFTIFNITVPAFQIAGGILLVSVATGMLASKG
                     EAYSGADLENIAVVPLAFPLSCGPGTITTVILLASGPEGIWGIVAVFLGIIVALALSY
                     IGMLYGPRIFALIGEGGLRVVPKLMAIIVLAIAIQFIINGIAAAMPQLLANFDLAGLL
                     "
     gene            20644..21618
                     /locus_tag="PLI76_02625"
     CDS             20644..21618
                     /locus_tag="PLI76_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOI60954.1"
                     /translation="MIDIEIAFIQALQDQAAWLLILMQFFSFLGQPEFYLLIIPAFYW
                     CRDPRLGLRLGLLMGISGGLNEALKVVFHLPRPYWVSPDVRAFGSYPSFGLPSSHAQG
                     AVTFWGLIAAHVRRWWFWISAVAIILLIGISRIFLAVHFPADVIVGFAVGAGTLLLFL
                     ALEEPVGRRIARLPLSGQVFVALAGSLALALVSVSALATLGDWQVPASWAAGALERSG
                     QPIAPLSLRDAATASGLLFGFAAGAAVAWPKRGGMCAVGKWSVRLLRYIFGILVTGAI
                     WFGFGLLIPKDPGLTAFALLYIQAAAAGAWVSFGAPAAFAYLNLADRS"
     gene            21670..22131
                     /locus_tag="PLI76_02630"
     CDS             21670..22131
                     /locus_tag="PLI76_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOI60955.1"
                     /translation="MLVEGIALGWILIVLGAVLLLVEVTQPGFFIAVPATVLIILGVL
                     LLLGIDVLSSPAGLIIGVVAAIAAASITVWAYSRLTPERKPPTTLSRDSLVGLEGMVV
                     APVDAVTLSGKVRLGSTEWSARSGSGEIIPAGRRVIVIHSEGVHVVVKEVV"
     gene            22133..>22452
                     /locus_tag="PLI76_02635"
     CDS             22133..>22452
                     /locus_tag="PLI76_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOI60956.1"
                     /translation="MLPVGAIPWTGLVTIFLIIVIVIIFASGVVIVQPYEQGLQIRLG
                     QYIGRMNPGFRWVIPLITRVIKLDLRTEVMDVPRQEVITKDNSPTNVDAIVYVRVIDP
                     EKAY"
BASE COUNT         3796 a         7151 c         7153 g         4252 t
ORIGIN      
        1 gaccagaggt cgccgggggt tttgagcggg gcagagcccc tttcggtcgg cgcaggggga
       61 ttatctgttc tctcgtccgt cgcgataccg gcagattttt tgcccccggc catctgacct
      121 cactacgtcc agggcagaag gagatccggc ccggagcagg agagacagag tgcgcatgag
      181 agagaggatc aaagagaccg atcagggaat ccagggggag gtcgacgtcc ggaatttcga
      241 ccggatgcag aggcacctgc gggatagagg cctgattgcg accgacgaga tcatccggtc
      301 cgggatcgcc ggcggccttg cgctcgaact cggcccggga cccgggtacc tgggccttga
      361 gtggctccag agaacagacg aaaccagcct gaagggggtt gagatcagcc ggcacatgat
      421 cgatgtcgcc cgaaaaaacg cccgggaata cggccttgca gcaagggtcg aatacacgct
      481 cggccgggtc gaggcaatcc cgtttgcgga tgcggcgttc gatgcggtct tctcaaatgg
      541 ttccttacac gaatggagca aacctgagag ggcgtttcac gagattcatc gcatcctgaa
      601 gcccggcggc aggtggtata tcaccgacct gaagagggac atgaaccccg ccgtgatctg
      661 gttcatgaag gtcagcgtca ggccggccga gatgcggccg gggctgcaga catcgatcga
      721 tgccgcatac acccgaagcg agcttgagac gatcctggcc ggaacaccgc ttgaatcctc
      781 atctctctcc gagaacccgt tcgggttgag ggttttaggc gtgaaataac catcgttaga
      841 aaaaccgggg gcccggggtc actccccgcc cccctgttcc cgtgcgatcc gttcctcgcc
      901 tttcttcgat cgttcgtgct ccgcccgcgg cgtcggggtc tttggcgccc ccggcggccg
      961 gccgggatcc cgcccctcgt tgccgagctc ggccgggacc gggctgtaga cgatatcggt
     1021 ccccttatcc tcgaacggta tctcctcatc gggttgctcg tcccgtctcc tcctccgctc
     1081 ttctgccata cgcatcactc ccgggtgatc cctgccggaa ggttccggac ccataaaagt
     1141 ctaccggcgc cggagccagc ctccggccac cgcgaccgcg ccggccccga tgagcgcccc
     1201 cgcccccgcg gcctgggtgg gggttgccgc gggtgtcgcc ttttctgccg gcgtcgtggt
     1261 ggcctctacc ggcacatcgg ccgtccggaa tacgaaaacc cgctcccccg cgcctgcggc
     1321 gatgtaaccg ccgtttgcgg agagggcgac cgacgtcacg ggcgcaccgg cgtcaaacga
     1381 ccagagcacg cccccatcgc ccgagagaag gcgcaaagtt ccgccggcgg tcccggccgc
     1441 gacggcctct ccctgctccg atacgtctac ggcaaggacg ccgctttcgg tatggttctg
     1501 ccagataagg ccctcccgcg gccggtagca atccaggtcg cccccttgct tcccgatgat
     1561 gacgaagcgt ccgtcggccg ggagggcgag cgcgaggatg ggtgccccgg cctgcacaac
     1621 ccaggggagt cccccgctcc tcccgtagac ctggaggacc cggtcccgtc ctccggcggc
     1681 gacggtcgtt ccgtcgcggg aggcgtcgac cgcggtgacc gggccgtacg cgatcccctg
     1741 ccagagcagg ttcccccggc tgccgatgag atgaatgaca ccgttgtcgc ttccggcggc
     1801 gatgtagttg ccgtcggccg taaaagcaat ggacgatacg gttgccccgg ctcctgtcga
     1861 gaagttccag ccctccttac cctggtggtc aaagagggtg acgcggctgc tccccaccgc
     1921 gagggaccgg ccgtccccgg agaaggcaag atcacgaacg ggtcctgtga ccctttcagt
     1981 ccagtagcgg ttgccgcttg ccccatcaag caggatgacg tcgcccgcgt tggtcccggc
     2041 ggctacatag ccccttgcat cggcgacggc aaccgccgtt accggatccc cggcgctcgt
     2101 atcccaggcg accgaaccgt cctgccgcag gcaggcgatc atccctccgt cggtccctgc
     2161 gaccgcgtat cggccgtctg cgctgatcgc aacgtcgttg acggcgcttc ccatcgatac
     2221 ccccccgccg gcaaccatcg gggccaggac ggcggcaagg acgatcatca gtgctgttct
     2281 tctcatggta acgttctctc ccatgccttc tttctcggag agttccttaa attgtttcac
     2341 catgctccgg agaaggttga aggcagccgg tgagcaacca atcccccggg tgagcatcac
     2401 gataaaacga gcagaagaag cggtcgcccg cttcatgcag ggctacaact gtgcgcaggc
     2461 agtcagttcg gtcttctccc acgacgccgg ggtgcccgag gagaccatcc tccgggcggc
     2521 aaccgggttc ggcggcggga tggggcgcac cgggggcgct tgcggggcgg tgtcgggggc
     2581 agtcctcgtc atcgggctca tggtcgggag tgccggaccg gatgagaaag aggcgaagga
     2641 cctgacctac gacctcacgc gagagttcgt cgagcagttt gccgaaagga ccggcagtgt
     2701 ctcctgcacg gaactcctcg gctgcgacct ctcgaccgat gaggggcgag cgcgggcccg
     2761 tagagataac ctcacccgga ccctctgccc ccggtacatc cggaatgcgg tcgatatcct
     2821 cgaagaggtc ctcgcacccg tcatttccgg gcggcccgcc agccggtgag agggccgggg
     2881 agggacggca gccggagcat ccgcgcgatc tcgccggtgc cgaggttctt tgccgtgagt
     2941 gcccgtttct cgaccaggtt ccaggagagg aacgcgagag cgaacgtcac ggcaaacgag
     3001 agcccgaaga gcgccgggag gaggagcgtg tttgccgtga ccaggatcag cgtctgctga
     3061 accgggtagt ggtagatgta gatgccgtag gagaagtcgc ccgacctccc gaaggtgttc
     3121 aggtagggga tggggagatg cgcggcgtag atggtgaggt aggggatgca gaccaccccg
     3181 gcgacggttg ggtaaggcgt cagggtcgag agaccgagca ggaggagcag cgccccggcg
     3241 acggccggcc ggtaggtgat ccgctcccgg tgaaggtaga ggtatgcccc gatcaggaag
     3301 tagagggtga accggacctt cgccatccgg gggtcgtcga accagtatat ccagaccagg
     3361 atgtttacga cggcaagggc gggaatggcg ccccatcgcc ggaggagccc ggcaatcccg
     3421 aggagggcga cgaccccgta catggcgacc tcgacgggta tcgtccagag cgagccgttg
     3481 acgtaggtcc acgggttctc ctggaagagg cctatcgcgg atccgtcctc gaagaacggt
     3541 atggtcgcaa gccccgccgg ggagaagaga gcgttaaagt actcccccgg ggaaagggtc
     3601 gtcatcagcg gaccgataac aaagagcgtg atgagaaccg caggaataag cgccgggacg
     3661 acccggagga accgtttcca ggcgaaccgg agcggtgatg cggtcgactc ccagctcgcg
     3721 gcgatcaggt aaccgctcgt gacgaggagg acggcaagtc ccgcctgtcc catgagcacg
     3781 aacggattgg aaagccctat gcctgtgtag ccaagcctga gcgcgtaggc gtgcaggatg
     3841 acgatcgcca ccgccgcagc gaaccgcagg aagtcgaagt tgttcgagaa ccgccccccc
     3901 ggaacgctcc cccctccgcc ggccgctccc gggcgttctg tattcatggc agtcggattc
     3961 ctttcctcat tgtacagcga caatcgttaa atatttagtc aaaatattct ttggttctcg
     4021 tgccgccggc aacaaccctt aaatatgcgg ccagaacatc ctcctcaatg gacccgctgg
     4081 tcgtcgccac gttcgccgtc gtcatcctgc tcgagatcat cgtcccgctc gcgctcggtt
     4141 actggattgt ccggaggttc ggcgttccct ggcggatctt tctcttcggt gccctcttct
     4201 tcatcgccgt gcaggtcgtc cacaccccgc tcgtcctcgt gacgcagggc ccgctctacc
     4261 tcgcccttct gccgtccggc acgacaatgg cccttgccgg ccttgccgtc tatctcggcc
     4321 tcatggccgg cctctttgaa gagatcggcc gctacgtcgt ctaccgttac tacttccggc
     4381 ggcggggtat ccccctcacc cgcgagaccg gcctccagtt cggggccggg tggggcggcg
     4441 tcgagagcat atttgtcgcg ctcctcgtcc tttcgagcat ggtctcctac atcgccctca
     4501 cgagcgacgg cggaatggtc ccgctccccg acgaccccgc ggtccaggca caggtcgaga
     4561 ccctcaaggc cctcacgccg ctcgatatcc tgcccggcct tgccgagcgg atgatgacga
     4621 tcaccctcca cgtcgcctgg tcgctcctcg tcctcgcctc gctcgtctac ggcgaaagat
     4681 tgctcctcgt cctcgccgtc ctctggcacg ccgccgtcga tgccgcggcc gtctaccttg
     4741 ccgcgacgat gggtattctc gtgaccgagg cggtggtatt cgtctttgcg gtgatcggtc
     4801 tcatctatat cctgtgggag tggcggcgga tgggggcaag agcggcctcc tagccgcccc
     4861 actcgtgcag gtagccccgg cgttcgagcg gttttccgtc cgcgaggacc acccgctcgg
     4921 gtatgaccct gccgatgacg tgcgtccgaa cgcccgggac cgggaggata tcggctggcg
     4981 cggtgaagag aagttcaaaa tcccctccgc cgtggagagc gagttcccgc ccctcctgtt
     5041 ccgggacgcc ggcagggagg gggagacggg cggtctcgat ggcaaacccg cagtcgttca
     5101 ccgcgagaag gtcgtagagc gagagggcga ggccgtcgga tatatccatc atcgccgaga
     5161 cgccggcacg gccgaggctc tcaccctccg ccacccgggg ttgcggttcg agaagttctt
     5221 tcctgtgctg ctcgtagccg gcaagggccg cctcggcctc cccgagcggc ccggtgaccg
     5281 tgatgacgtc tcccggccgc gccccccgcc ttcgagcgat atgctcgggc ctgaccgtcc
     5341 caaggcccgt gctcacgatc gtcagttccc ggtgggcatc gagatccccg cccacgagct
     5401 cggcgccgaa cgccgtgcag cagtcccggg cgcccgtcat gataccggcg agccgttccg
     5461 gcctatcaag gccgaccgcg aggaggacct gccccggcac cgcccccatg ctcgctatat
     5521 ccgagagcgt gaccgccacc gccatccagc ccacctgcca gtcggtcatc ccggcgggaa
     5581 aatcggtcgt ctcgtggagc atatcggtgc tcgcgaccat aaggagattt ccgcagggga
     5641 tgacggcgca gtcgtcgctg agccgctccg ggtcgatgat cgtcccgatc agccggtgca
     5701 ggccccgttc atccaccgcg acgcacctcc ttctcccgct cgctccggta ctctttgaag
     5761 aggtactcga cccgctcggc ctcctgctcc cgccggtact ctttctgccc ctcctcccat
     5821 tcgccgagtg cctcgctgac ggccccggtc tttgccgccc cggtcctgcc ccgtatatcc
     5881 ggctcgatgg cgccggccgg gagaaggggg aggccggcct cccgggcgac ccggagaagg
     5941 tgcgggtcgg gaggctcccc gccgacgacc agcgcccgca cccccatccc cgcaagatcc
     6001 ttcaccacgc cccgccccca gccgtgcgcc cgggggacgt agaggacgtc gcccgcggcg
     6061 atgccgatct cctcctggag agcccgcacc ccgtcccggg tgagggagtc gagcaccttg
     6121 agcggggtgt agtcctcgga gacctcaagc tcggcaacgg tctgcatccg ttcgaggcgt
     6181 ttcttcagcc gccgggacct ccgccgttcg ccacggagct gctcgcgcag gctctcgatc
     6241 gttgcgtcct tcgtcgcgag ttccgcatcc cgcctgacct tcctctcggt cgcggagcgt
     6301 gcccgtcgca ggtcctgttg cagtcgctcc acctcgcggt cgcgttcccg gaggtcttcc
     6361 tgcagttcct ggacgtaact cctgagccgc ttcaccatac cgtcgagcgc catcacccgt
     6421 tcgtcctcga tcggccgttc ctggaccgct gccgccggtt cggcctctcg cggggccgcc
     6481 ggagcgccct gcaggtccgt gaggaccgca tcaagcggct ggccccggag cacccgcgcc
     6541 cgcacttcat cgaggtcgac ccccggccgc actcgcttca cgatattgcg gaacttgttc
     6601 tggaggctcc ggaatgcctc cagggccgct gagagggcgt cgcgctcgtg gtcgttcgtg
     6661 taggagaacc gggcggtcag gtcgtacttc gcctccacgg agagcgactg cttcggcgta
     6721 taggggatgg cgttgaacgc ccggcgaatc ttctcgaccg agtaaggcat ctcgcggacg
     6781 tcggaggcga tgatgagcgg ttttccggcg ccgtagagtt cctcgacgat atcggacatc
     6841 gtcatctggc gggaactggt gaggagaacc aggttgccgt cgagatcgac ggcggcgata
     6901 ccggtcgtcg tccccgggtc gagcccgacg atcaggtagc gggggcggct ggaacgcggt
     6961 tcgaaccgta tccggtcgag ttctctcccg gccacccgga cctggacatc ggcgccccgg
     7021 gaggagtgga ccggcaccct gtcccggggc gcaacgaccc ggaaaagaac ccgcgagaac
     7081 ccgccgaagg ccttcgtctc cttcttctcg tagtcgaggc cggcacccct gagccgggcc
     7141 tcgatctcgc gggccttctg catcacggca ccgtggatct tccttgcata ccggttctgg
     7201 ctccacccgc cccgcccggg cgagcggtgc cgactcacca cgatgtcggt cgtgttctca
     7261 aacgctatca cctcgacgcc cgccccgaga gaggccacct gtgcggtggt ccgggcctcg
     7321 gcgtaggggt cgaatttatt aaaactgatg ttgtagcgtg ctgccacctt tccgaggctc
     7381 tccgtcctct cgccgccggt cacctggacg agttttgtcg agggcgggag cgcctgcagg
     7441 aaggtgcgga gctcgtgctg gtcggcggcg atctcctgga tgctgtccac cgcgaggatg
     7501 tcgggttcct cggcggcaag gatacgctgg agccggaaac cggtgacctc ctcgacgccc
     7561 gtaatctcgc cgtcctctgc ccgggcaagg gcgtagacgg gccggcgcgt gcgggaacgg
     7621 accgaacctt tgatgatgtc gataccaaag accttcactc caccacccgt gccagtgctt
     7681 cttcgaaggc gtactcgatg ccgtatttcc ttttgaagta ctggaggatg ttcccgatat
     7741 cccgtgcgag gatctcgccg gcgttcgggt gcgctatctc tacccactgc ggccagtcga
     7801 tcagccagca ctgcttctcg tcgaccatga cgttgaactc gcttaagtcg ccgtggataa
     7861 tgccgaggcg gtaggcctcg cggatgttat cgaggatctc gtcgaggatg atctccggtt
     7921 ccgcaagtgc cgctcgcgag aggttgacgc cggggacgaa cgacatcgcc acgacgtgcc
     7981 gattctggtc gatagggagc ggcaccgaga ccgcggggtg gagggtcttc aaggcatcgt
     8041 actccatctt tgccgagtgg ctcgaagcaa agagccaggg gcagtgcccg gcctccggca
     8101 tgtagccgcg cttcaagcgg gcggactgaa acgacctctg cccgacccgg tggaacttca
     8161 gcacgacgac gccaaggccc atcgcctcgt aaacctccga ctctttcccg acgccgatga
     8221 ggcagccgag tgcctggacg gtcccgcggc gggtgagggt gtggagggcc agggtgtcgt
     8281 agccgtcaaa gacgagagcg tagccttcgt agggtgtctt ctcgtacctg accatgccca
     8341 tggccatcag cctgcccagc cggtaggcga gctcggactc ggagaacccc gttgccggcc
     8401 tgagcacctc gagcggcacc cactggtagc ggcgcatgag acgttcgagg gcgagcagga
     8461 tccggagttc gtaggcgtgc agggatcgca cgtattctgc aggaacaggc atcttctaca
     8521 ccatttatgt gccacggtga taaaagaatg atcgaggaaa gatgcagtgc agcaagtgcc
     8581 gccgcgacgc ggtcatatac cagcgatact cggggctgca cctctgcgag cagcacttca
     8641 accgcgatct cgaggcgaag gcaaaacggg ccatccggga acaccgctgg atcgcgtcgg
     8701 gggatacggt ggcggtggcg ctctcggggg gaaaggactc aagcgccgtc ctctacttcc
     8761 tccaccggct cctccacgag cgccgcgatg tccggctgat ggcgataacc gtggatgagg
     8821 ggataggggg ctaccgcgat ccggcccggg cccgggcgat cgccgaacgc atcggcgttc
     8881 cctgggtgac ggcgtcattc cgtgaggagt atggggtcac gctcgatgag attgtggcac
     8941 gaaaggggac ggggctctcc tgctcctact gcggcgtgct ccggcgggcg ctgttgaacc
     9001 gggtcgcccg cgaggaaggc gtcacgaagt tcgcctacgg gttcaacctg gacgacgagg
     9061 cgcagtcggt gttgatgaac gtcctccggg gcgatcccta ccggctcacc cgcccggtgc
     9121 gggaggtggc agggtttgtc ccccggatca agccgttcat gtatataccg gaacgcgagg
     9181 tggcgctcta cgccttcttc cacgtcgaag gatttgacct tgcgggctgc ccctacgcgg
     9241 cagatgccct ccggggggac gtccggggga tcctggatgc ctacacctac cgccacccgg
     9301 cgacgaagta ctcgctcgtc aacctgggcg aggccctccg gaacccggag caaccgccgg
     9361 agggaggact ccgggtctgc gaacggtgcg gcgagccgtg cggggaggtc tgccgggcct
     9421 gccaggtcct cgacgaggtg cggaggggtg cctgaaggcc cctcacagca tcggcaggca
     9481 accgccggtc acgccggcga atatctccat gaactgttcg gggtggttga ggaaggcgac
     9541 cgaccggggg cggccgatca ggtcgtagag cccggtgccc ttctcgatac cgagttcggg
     9601 aacctctgtc gggtcgcagt agcgcaccgg cgacgcgtcg gggtagtgcg ggtttttcac
     9661 gagcgcccct ccctccgtct cgtagtaggc agcgccccgt ttttcggcaa aaggaccgta
     9721 gttgcttgag aactccgccg agacgaggtt tgccatcaca agatcgacct ttccggggtt
     9781 gatggtcacg tggccgtacc ccggcgggat caggatctta tccccggcga tgccccggat
     9841 catcaccacg tcgtcggcac ttctggtctg gaggaggtag tgccccgtcc ccgagatcac
     9901 ctcgtagatc tccgggtaga agaccccggc cgggtttaat gtgtggtagt gtcccatcgt
     9961 cttgacgtgc tccccgcaga gggtcgccgc tggaatgacg gtgatgtcgt agcggatgtg
    10021 ctgctgtcgg agccacgccc ggtcgctctc gtttttggcg aggtcgcggt acataaaata
    10081 gaggggttga tcgaaggtgc agccgggatc tgcgagaacc tcccgcatct catcgatcgt
    10141 ccttgcctgc ggttccggtt tgggcccgtc ccagtatccg ttcatcactt tttcttacgg
    10201 tacgcgaata tataataccc tgcaccgatg atgacgaccg caattacagc gaggaatacc
    10261 ggattggtga agagggtgcc ttctttcttc tcgagtgtga cctgcacctt catggtatcc
    10321 gatatcttac tgttgtcaag gtcgtcgcgg taacggatct cggagtcgat accgtaattc
    10381 ttcagggtcg catcggcatc gatgctcacc tcaaaccgtg ccgtcgcggt ctcgccggga
    10441 gcaaggtcgc ccaggtaggc ggtgtcgtcg ctgctggtga aggggtcgac ggcgctgatc
    10501 cgggcctgag cactgtagac ctttgccgcc cctgcgtttc tgtacacgac ctcaaggatg
    10561 ctcttgccgc ccgggtggag cgtcaccgtc ggcgaggcga catcaaagtc tatcttgccg
    10621 ccgaccggaa ggccgatcgt caccggccgg gaggccgttg acttcccctc gtagtcctcg
    10681 taggcgacca cgatgtccag cggataagac tggggttcgg cgctatcggc gaccgcgacc
    10741 ttgaacgtta catccaccgt ctcccctggc gcaaacgtcc cgatgtaggc gttgctgtcg
    10801 gtggggatga gcgggcttgc gccgtttctc gcgactttcg cgaccgcctt atctgctgcg
    10861 tcgtgcccga tgttcttcaa ggtcatagag acgtagcctt ccgtgccgac gtttaacgat
    10921 cgggtctgca cgttcaggac ctcgacgcgg aggccaggcg tgaccaggac ggtcaggggc
    10981 agggtctcgg tcttcttctg gtaattgtag cggaggaggt cgccataatc ctctgcatcc
    11041 tgcaggtagg tgtaggtcac ctcaagcggg agggtgtagg tgcccggcgc tgccgagtct
    11101 gccaccttca cgttgaacgt ggcgggcagg gctgcgcccc ccatgatgtc gcttaagaac
    11161 tgggggtccg tcttgacggt gaacggggca tcgttgctct tgagcgctac ggtgaccagc
    11221 ttcgcggtat tcggcaggtc gtcggggggg agcgacggaa tggcgatctt atgagtattc
    11281 aagccgctat tcgatatgat gaccgtcagg ccggtctcgg cgcccggtgc aaactcattg
    11341 gtgccggcga tcgttgccga aagttctggg tttccataaa ggtacttggc ccccgccgcc
    11401 gaccccggca cagccaggag tgccagcagg agcatcagta ccggtacgtg ctgccaacgc
    11461 atctcttcac cttgttgtta ttttagcaac aacatttatt atcaggccgt atatatatgt
    11521 gatggatgac ggctccccgg gatattccaa acgacctcat cgaatctctc aagtcgcttg
    11581 ggcttacaag gtatgaggcc ctcgtctaca tcggcctcct caggacgacg ggtgcgacgg
    11641 cgacagagat ccacgagacg tccggggtgc cgcgcgcgtc cgtctacccg gtcctcgacc
    11701 ggctcgtcca gaaagaactg gtcagcgtct ctcacaccac gcccaaacgg ttcgacgcgg
    11761 ttccgccggg ccagggtatc gagaacctca tgcgccggat cgaggatgat gcggagagtg
    11821 ctcgttcagc tctcgagagg ctctaccggg agaagcggcc ggaagcgcga gggaatcagg
    11881 aaccgatctg gacgatctac ggggaagaga acatcagagc ccgcctctcc gatatgttcc
    11941 ttggcgcgga gagaagagtg gagatgctcg tagcccggga tctcctcgtc gaacacgtac
    12001 ttgcgctgct cggccccgtt cccgaatcgg tgcaggtcga tatcatcacc gatacctggg
    12061 agggggagca gccttcccgg ttcaggatcc atgctcatcc ccccggcgcg caccagaaac
    12121 cacacacgtc gccgggaaaa ggcctgcgct acgagagctc cggcatcttc ctcgtggacg
    12181 acgtccgggc gctggtctgg atgggctcaa gcggcgagca gccttcggcg ctctactcgg
    12241 agtctaccgg actcgtccag ttcgtcaggc gctatctcac cagcatcatg gagtgggtgg
    12301 gagcgaccgg tcagtaatcc tcgaggtagc ccatctcccg gatatcgatc acccctttga
    12361 gtgcccgggt cacgatgggc tttcctgcct ccctgacggc ggccatggcg ttcgtgcccc
    12421 cgaccatcgc cacgcccacg tactgcgggc tgaccggaac cccgagcagg ggggcgttcg
    12481 gggcaccgac gtccaggacg ccggaaaacc cgatagccgc gagttcgtcg agcaccgtgc
    12541 ccacgagcgg ttccgcttcc atatggcact cccggatgtt tgcaagtatg ctgccgttcc
    12601 cgtgatgcat gacgtcgagg atcgacgtgg tctcctggga tatcaggacc tcaagggggt
    12661 cgagcgtggt gtcgcgatag aggatcatgc tcgtgaaccg ccggggaacc cgcgcctcga
    12721 tctcgacaac cccgcctccg atcgggttta acgggatgcc gcgccggagg aggagggcat
    12781 cgagggtgat gctgcacatc gtgcagacgg cgtgccggcc gtccggcacg gtgtagccgc
    12841 cgatctcttc ccctgcttcg gcgaccctga tccggtcgct gatggtgatc cccgtccggt
    12901 gggcctcttt catgacggag agcgcgaact caaggtcgcg gttctcgatg agcgagaggt
    12961 tgtagatgat cgtcccggtg tcctcctccg ggcgataggt caccctgagc gcgtactcct
    13021 cgatgcggtg gttggtgaac ttcaggggga catgcattat cagatctctt gtgcgatcag
    13081 ggaaaagggt tcctttcggg gtgctccggc gggcggcggg ggagatggtg gatgaaccgg
    13141 catgtcgagc ggttcagaaa tgaggcgggc aggccggaga ccgaccccgg tcgcggggtg
    13201 acgcgcgact ccgtggcagg ctctccggag agtaattccc tccccctcac ccggccccgg
    13261 atgcaaaact tgttctacct gtctgtgata ttatacgatg aatggagagt gggactcggg
    13321 agaaagcccg gaatgtgctg aagcggatac tcgaagcggc ctccttcgcc gtggcggagg
    13381 tcgacgaccc gctggtttta tccggaatca gaggcgatac cgccctgctg gtgctctgct
    13441 ctgatgaccc ggagacaatc agaaagtttg attcgatgaa ctaccgcctg cgcaccggcg
    13501 atgacgacct ggtctgcagg aaactgctct ttaccctcaa cccggcggtg cagacggagg
    13561 actgcatgcg gtggggtgcc gatgagttca ccctctacgc aggcagggct gcgcttgcga
    13621 aggtgctcgg gcagaccctg gtcctggata tcggccggcc gcccccggcc acccgcggtg
    13681 agtcggtaga ggacgccccc gtggccgtgg aggaagagcc ggaagcggga ccggagctgc
    13741 cccatcttcc cgtccggatc accgaaaagc gggcctgcgg catcgccggg ctgcacggtt
    13801 cggcgaagtg ccggttcatc ccctgctgga actaccgcta cgtgagcacc ggcgaacgcg
    13861 aggtgaaaga tagactggtc tcctttgacg ccgagggcgc aggatccatc aacgccatca
    13921 acggcatgaa gatggagatc gaccaggggt ctatcgaacg ggaacccgtc cctttcggct
    13981 ccgagatcgt gcccgcccgg ctcgtgcggg aggacgtcga ggggcagatc gtgcgggagg
    14041 ttatcgatcg gctcacccag cggcttcggt tccgttacga gaaaggcgac gccatcttct
    14101 acgaggaaaa gaccctcaaa cccgaccgga gcaacatcaa ggtcgacctt gagatcatat
    14161 acgtgccggt ctggcaggtg cggggcggga gcaggattgt cgaagtcaac gcgttcacgg
    14221 gcgaggtcct ctcgatgccg atggatgagg gcgtcgaact cctgtaggga accatgattg
    14281 tcgtcatcgg cggtgggccc gcaggaagga tcggggcgat gcatcttgcg catgcgggaa
    14341 gggaggtccg gctcatcgag aagcgtaaga tcggcggaca atgcctgcac gaccggtgca
    14401 tgaccatatg cgctctaaac gacatcgccc gactcatcga gtacgcccgg acccagaagg
    14461 atctcggtat actcgactcg gtcccgaccg tctcttaccc ggcggtcgta cggagaatgc
    14521 gcgatgtcca ggagaaactc tccacggtcc tcgacgttga aacccgccgg gcgggagtcg
    14581 atatcgtcta cggcgccgag ggcagactgg agggggaccg ggtctttgtc gatgacgaag
    14641 aggtccgggc cgacgcggtc cttgcggcca cagggtcgcg ggcggcgatc cccgatatcc
    14701 ccggcaccga tctcgccggg gtctacacct accagacgct ccccgatatg gaagaactcc
    14761 cgtgccgcat ggtcatcatc ggcggcgggg tggtggcggc ggagtttgcc cacgtattcc
    14821 gggcattcgg ttccaatgtc gacatcatcg cccggcacga ggtgctccgc ggcctcgacg
    14881 gaaaggtccg cgccgccgcg atacgcgacc tctccggggt agagatccgc gaagagactg
    14941 cggttctccg gatcgagggc agcgaccggg tccgatcggt cctgctcgcc ggcggcgagg
    15001 agatcgaggc cgacgccgtc cttctcgcca ccgggcttct cccccggtcg gagtcgctgc
    15061 aggggctcaa aaaaggtccg gatggcgcgg tcatcgtcga ccggcggatg cgcacgagcg
    15121 tggagggggt ctacgccgcc ggcgacgtca tcggcccacc ctacctcacc cccgccgccc
    15181 gccgggaggg cacggtggcc gccgagaaca tactcggtca cccagccatc atggattata
    15241 caggcattcc gcaggcgatg agcctccgtt acgactacgc gtttgccacg accggggccg
    15301 acggagaggg cgtcaccctc tctgccccgg cccccgccgg ccccggttcg ttctgggacg
    15361 tggcgggagg ctgggcgggg cttgcgcagg tccggatcga cccggagacc ggtcggctca
    15421 tcggggccgc ggcctgcgtc cccggggctg caaccctcct ctcctacgtc ggctacctga
    15481 tgaagcgcgg catcaccgcc agtgacttcg acgatatcgt cgaggtccac ccatcgaccg
    15541 acggcgtcta cgggcttgcc cgctatgctg ccgatatgct ccggaagaga gatgggtaag
    15601 atgtcccggc gctcttagat ctgccggaac atctgcccga acttcggtat gaaatctttc
    15661 tttcgggaca tgacgccctt gagccggacg ggctgatccc ggagacccag tttggcgatg
    15721 caggcttcat ccccggcggc gaagaggtcg cttgcgtcgc caaagacgtt cgtgaagagg
    15781 gcaaagaagc agtcgacccc catcgtcgtc cgcaggcggt cgagttcggt gcggatctcg
    15841 ccagcctcgc tctctgcaaa ctcaaacgac gacgtcatca cctgcgagac catgatgctc
    15901 cgtccgaaga ggttgtagcg cttcatgtcc cgggtgagga gctcgtcctt cgggagggca
    15961 tcaaggtcca tacccttctg gatcagtttc ggcccgaact cggccgggtc gacaccggcg
    16021 atctccgcaa ggtagtctgc cgcccggata tccacgggcg tcgtcgtcga gagcttcatc
    16081 accatcgtat ccgagaggat ccccgcaagg agaagcccgg cggtcggcgg cgtgggctcg
    16141 atagaggcct cgatgaactt gttcgcgacg atcgtcgatg tcgatcccag gggatcgttg
    16201 aggaacttca ccggctttaa ggtggatatc gcgccgaggc gatgatggtc gatgatctcg
    16261 aggatatccg cctgctctat cccgtccacg gcctgggagt actcgttgtg atcgagcagg
    16321 atcaccgact tctggacgtc ctgcatcagt gccgtccggg atacgagccc gatatggcgc
    16381 cggtcctccc cgacgacgca ggcggtcctg aacctcgagc tcgagacgac ccctttcgcg
    16441 tactcaagcg aatcttccag gcgcaccgtg gggacatcgg tctccatgat catgcgcgcg
    16501 gggagagaga ggctgatcat cttgccgacg ctgaaggcat caagcgtcgt cgcgaggacc
    16561 gaggcccccc gcgcccgggc ggcggcgatc acccgctcac ccaccggcgc gccctcggcg
    16621 atgacgagtg cggcgactcc tgccgatatc agggcgagct gggccggttc gttgtccccg
    16681 acgatcgcga tgtcgtccgg cgtcatccgc gagagagtga cgtgcagggc atcgatgacg
    16741 gtgtagaccc gcccctcccc gagcacctcg tgcgccgata cgaccgtccg ggcatcgagg
    16801 atgcgggcaa gggtcgcaag gggcatcggg acgagcgaga gctgctcgcg ggggttcttt
    16861 cggacgtagg cccgggcaag gccgtactcg ctgacaagcc cgaccagggt ctcttcgtca
    16921 tccgtgatcg gtatattccg catatcgtag gtatccatca gggcggcgac gtcgactgtc
    16981 ggaacatcct gccgggcgct ccgggtatcg agcggtatat ccgatacgct tggttcgacg
    17041 cttgcgatat agaccggagg ctcgacgttg aacgtctcaa gcgcaaaccg ggtctccgcg
    17101 ttcacctccc cgcaccgggc ggggatgtac ttccccggtt cggcgtggtt gcggagctct
    17161 gcgtagccga tgacgctgca gatgctgtcg gtgtcgggct gcctgtggcc gatgatgacg
    17221 atcgtgtgtg gtcccatgct ctcctcggac ccccgcgggg cggtggttgt ctcaagagtt
    17281 gtcgcgctct gccgtcaaag gctttgtgcc ggatatccgg ggaaacgttg aagtagtggt
    17341 ttcctccaca ctccggcgtg gaaacccctc gccccgggtc tgctgcagat ccgctgatcc
    17401 tggtgtgctc tcttgaactc cgcctgccgc cgcggcctcn nnnnnnnnnn nnnnnnnnnn
    17461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17521 nnnnnnnnnn nnnnnnnnng cagatccgct gatcctggtg tgctctcttg aactccgcct
    17581 gccgccgcgg cctcaggagg ccgcaacggt gagcgatgag cccctcctcc ggttcgaggc
    17641 ggtcagcaag gtctatcccc tcccggcagg cgatgtcgtg gcgctcgatc gcgtctccct
    17701 caccgttgat gcgggcgagt tcatcgccgt gatggggccc tcgggctccg ggaaatcgac
    17761 gctcttgcac ctgatgggct gccttgacgt cccgaccggc gggaagatct ctctctccgg
    17821 gcaggacata tcggggatga gcgacgacga cctcacaggg ctccggcgcg accgtatcgg
    17881 gtttatcttc cagcagttca acctcatacc gcttctctcg gcggtcgaga acgtcgagtt
    17941 tcccctcacc ctcaccatcg gccgggagga gagccggcgc cgggcgatcg aggtcatgcg
    18001 ggcggtgcat ctcgacgacg ccctctttac ccacaggccg gccgagctct ccggcggcga
    18061 gcagcagcgg gttgcgatcg ctcgtgcgct cgtcaacaac cccgatcttc tcctctgcga
    18121 cgaaccgacc ggaaacctgg ataggaagac cgggaccgcg atcatggaga tcctcgaggc
    18181 agagaaccgg gagggcaaga cggtcatcgt tgtcacccac gacccggaag tcgctacata
    18241 cgcccgccgc acgatccgga tcgtggacgg gaaagtggta tgatcttcct ctccttcgct
    18301 cttcggagcc cgatcgctcc acccggccat cgagatcatc gtcgccgtta cggggccttc
    18361 acgttcggcg agatgttcgc cgccttcaac acgactgcca ttggcgagat ctccttcgcg
    18421 gtggcgctcg gtatcggagc gagtctcacc gacgggctct ctccctcgtg gaaagcggcg
    18481 cagttgaacc ccatcgaggt gctccgctac ggatgacggg cctggaggct atcccccggc
    18541 gccgccgtct gccctgtgcc gggtcggttc tcccctcccg gtgtccgccg cgatgccggg
    18601 cgaagatccc ggtggggtga gaccatgcgt aatttcatag tcatggagac cggaaactct
    18661 atgagcactt ataggatgat aacctcccta tcaggaagtg ctccgaccca tccggtcccc
    18721 aatacgatac caatacccgg ctgtgtggaa cgaatgttac agatgattac gtggcttaac
    18781 aagaacttca gctcgctcca gccgacgcga gcgatcgtca tgagggcact gcgccacctg
    18841 cgccccgcgg atcgaaagaa actcttctcc gaggatatac ccgagatgcg gaccgcggaa
    18901 gggcggtggt ttgaagcgat cgtctacgag atgatcctcg atctctcgct gaagaccgac
    18961 ctcatcctcg ctgtcgtggc ccggggcgcc gatggcccat cgaaggtccg gcgcgcacag
    19021 cttggccaga acggcctctt ttactcgaat atcggtgaca tcaaggtccg gggcaacggg
    19081 caggatctcg ccgaggtcga cctgatgctc gtcgaccaca cgggggcgct gacgtttggc
    19141 gagatcatca cgtctcctgc ggatttaaaa gaatttgaag cggagatccg ttacaaaaag
    19201 cagctcctgg gctatctcta cgggcagccg accgtgccgt tcctcctcat ctcttcagtc
    19261 gacatatcgc ggacggccgt cgtccgccgc ctcctcaaag aacccgacaa catcctcctc
    19321 accaccccga catgcgagga cctgaaatcc ctgatccgcc caagggacct gaagcggagc
    19381 ccgcccaggc agatccggca cgagaagctg atatcgatac aggatatcgc cccgcggcgg
    19441 ccgttcgact acaaggaact gcacgacgag cggttgcaga gcatcgtcgc tgccgtgacc
    19501 tcccaaaaag gcatcgaaaa actcggcaca ccggatgaga tcccgccgat cgtgaaaaaa
    19561 gtcctcttcg gcgggctcta cccttccgcc gtccggatgc tcgacgcccg gtatcccatc
    19621 cagatcaagg ggaagactta cgatccggat gcgatccaga agcagttctc aaaggtcgtc
    19681 ctcgcggtga atatccccga gtaccggccg atcatctacc tccggacccg ggacaagaag
    19741 gagtacctca agatggtgcc cagccgtgag ggggggttca agttcgagag ccggcggacg
    19801 ccgcacatgg ccgggttctt cctctggctc gagtccatcc ggacatcgct tggggcggag
    19861 ctgacgcagg aactcctcga tgcgttccct gcggtccacg tttctccggc cccggcggcc
    19921 ggaggatcgt aaggtaccta tggggccggg gcgcatgtat ctctatgttt atagggtgtg
    19981 gtgatccgat atggacctga tcgctgaact tgtcagtttt ttagggtttg gcctggttgc
    20041 gatagcgtcg atcaccgcgg tgatgaatcc ggcctccacg atcgccgtct acacggcgct
    20101 caccgagggg atgggcggga gcgagcgcat gaaggtcatc cagacatcca tgaagatagc
    20161 cttcatcgtg cttgccttct tcgccctcac aggccagctg atcttcacga tcttcaacat
    20221 caccgtcccg gcgttccaga tcgccggcgg tatcctcctg gtcagcgtgg cgaccggaat
    20281 gctcgcctcg aagggcgagg cctactcggg tgcggacctg gagaacatcg ccgtcgtccc
    20341 gctggcattc ccgctctcgt gcggcccggg gacgatcacg acggtgatcc tgcttgcgtc
    20401 gggaccggaa ggcatctggg gtatcgtcgc cgtcttcctc ggtatcatcg ttgctctcgc
    20461 cctctcctac atcgggatgc tctatgggcc gagaatcttc gccctcatcg gggagggagg
    20521 gctccgcgtc gttccgaaac tgatggcgat catcgtcctt gctatagcca tccagttcat
    20581 catcaacggt atcgctgcgg cgatgcccca gctactggca aactttgatc tcgccgggct
    20641 tctatgatcg atatcgagat cgcattcata caggctctcc aggatcaggc cgcatggctc
    20701 ctgatcctca tgcagttttt ttcattcctc gggcaaccgg agttctatct gctcatcatc
    20761 ccggcgtttt actggtgccg ggatccgcgc ctcggtctcc gcctcggcct tctcatgggt
    20821 atatcgggcg ggctcaatga ggctctcaag gttgtgtttc accttccccg cccctactgg
    20881 gtcagcccgg atgtccgggc gttcgggagt tatccatcgt tcgggctgcc gtcatcgcat
    20941 gcccagggag cggtgacgtt ctgggggctg atcgccgccc atgtccggcg gtggtggttc
    21001 tggatatctg cggttgcgat catccttctc atcggcatat cccgcatatt tcttgcggtg
    21061 catttcccgg ctgacgttat cgtggggttt gccgtcggcg ccggcaccct tcttctcttc
    21121 ctcgccctcg aagagccggt tggccgccgg atcgctcgcc tccctctttc agggcaggtg
    21181 ttcgtcgcgc ttgccggatc cctcgctctg gcgctggtct ccgtatccgc tctcgcgacc
    21241 ctcggggact ggcaggtccc ggcatcctgg gcggcggggg cgctcgagcg gtcggggcag
    21301 ccgatcgctc ccctctccct ccgggatgcc gccacggcgt cggggctttt gttcgggttt
    21361 gcggccggtg cggcggttgc gtggccgaaa cgggggggca tgtgcgccgt cgggaagtgg
    21421 tctgtccgcc tgctccgtta tatcttcggt atactggtga cgggggcgat ctggttcggg
    21481 ttcgggcttc tcatcccgaa agacccgggc ctcaccgcct tcgcccttct gtatatccag
    21541 gctgccgccg ccggtgcctg ggtctcgttt ggggctccgg cggcatttgc ctatttgaac
    21601 ctggcggatc gctcctgacg gacccccctt cccataccct ttttatcccg ccgggtccat
    21661 ctctctcgta tgcttgtaga ggggatcgct cttggctgga tactgatcgt gctcggggcg
    21721 gtgctgctcc tggtcgaggt cacccagccc gggttcttca tcgctgtccc tgcgacggtc
    21781 ctgatcatcc tcggcgtcct ccttctcctc ggcatcgacg tcctcagttc tcctgccggt
    21841 ctcatcatcg gggttgtggc ggcgatcgct gcagcctcga tcaccgtctg ggcgtatagt
    21901 cgcctcacgc ccgagaggaa accgccgacg accctctcac gggattcgct cgtcggcctc
    21961 gaggggatgg tggtcgcgcc ggtcgatgcg gtgacgcttt cggggaaggt gcggctcggc
    22021 agtacggaat ggagcgcgcg ttccggatcg ggggagatca taccggccgg gagaagggtt
    22081 atcgtcatcc actccgaggg cgtgcatgtt gttgtaaaag aggttgtgta aaatgcttcc
    22141 ggtaggtgcg attccgtgga cgggattggt cacgatcttc ctcatcatcg tgatcgtcat
    22201 catcttcgcc agcggcgtgg tgatcgtgca gccatacgaa caggggctcc agatacgcct
    22261 cggacagtat atcgggagga tgaatcccgg attccggtgg gttatcccgc tgatcacgag
    22321 ggtcataaag ctcgatctcc ggacggaggt gatggatgtc ccgcggcagg aggtgatcac
    22381 gaaggacaac tcgccgacga acgtggatgc gatcgtctac gtccgggtga tcgaccccga
    22441 gaaggcctac tt
//
LOCUS       DAOSEE010000019        21447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1065_length_21447_cov_225.635611, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000019 DAOSEE010000000
VERSION     DAOSEE010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 21447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21447
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1065_length_21447_cov_225.635611"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PLI76_02640"
     CDS             <1..449
                     /locus_tag="PLI76_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOI60957.1"
                     /translation="VCGGRLQPVLDATALAKELGMHVETVTLVIPGQNDSMDEMEMLI
                     RWVLDNLGPDTPMHFTRFHPDYRMLDARPTAVRTLEKIYERAKELGLNYPYLGNVGGH
                     PYENTYCPACGSLAIERSGYAVRIRGIDDHTCTECGGRIEYVSTVD"
     gene            430..921
                     /locus_tag="PLI76_02645"
     CDS             430..921
                     /locus_tag="PLI76_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HOI60958.1"
                     /translation="MSQQSTEQRRFLADRMLGTLTRYLRFMGYDTMSANSIAPGMARE
                     DTLLLEIATRDKRLLLTRDRELARRGGAQAVYIVSEDIMEQIRQIAGLGLIEPRVRMN
                     RCSICNTHLRPATAREVREAREVREEDREREFSWCPVCRKLYWMGSHGEHIEERLRES
                     FSP"
     gene            1114..2262
                     /locus_tag="PLI76_02650"
     CDS             1114..2262
                     /locus_tag="PLI76_02650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOI60959.1"
                     /translation="MKHLKIAFFCWESLYAERVGGLANAATNLAETLAQRHEVHFFTR
                     GEGRDAEINNVHYHYCRPAGEDIVRYCSDMSQKMLDRFGEFDRPAFDLLHFHDWHVVD
                     ALVRLRERETVFTYHSTEFGRSGNRYSDEWLFPAISGAERRGGEIAKRVTAVSSTLRR
                     EAMNLYDIPDWKIDVVQNGIIPGHYRADVDPEAVKQRYGFDPGAPLILFVGRLMWQKG
                     PDMLVDAAPRLLQEHADSQFAFVGDGQMRRGLEAQARSLPVRFLGRLDDADYVSLLNA
                     SDIVAIPSRNEPFGLVLLEAWSAGRCVVASDVGGLAENIEHGTDGVKVRPRPDSIARG
                     LSLASDSPALSLAMGRRGFDKVMEEFPWSRVRERMENSYRKARETLLC"
     gene            complement(2264..3322)
                     /locus_tag="PLI76_02655"
     CDS             complement(2264..3322)
                     /locus_tag="PLI76_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60960.1"
                     /translation="MLEVRLDITRLPGIVRELAPDTGDLVSRIYSWYVEPGNLVFPDA
                     MQAWVREKYGDIERQQIVRVTNDRTGESTLFNSVRARRPVLTPRAGETRDLEALAEGG
                     PFADPLNETPADTFGRIDGDHWITASNIARYDYLHAVIIAKEKNPYVVTEPQVADMVS
                     VAVRWCQEAHRADSRAVYPFIVWNYLWRAGASIVHNHAQVLLTHRPYAAPARLEQARE
                     EYRCRYGCEYYDDLFSAHVAIGLGFAYRGARVMAYLTPRKEHEVAIITESPHDIAPAL
                     AKVLACYRNIGVQSYNAAVYMPPLGEEGHYVTWVVDRGDLHSRTSDIGGMELYAGTPV
                     VTSDPFRLMEHLTAVMLP"
     gene            3472..6921
                     /locus_tag="PLI76_02660"
     CDS             3472..6921
                     /locus_tag="PLI76_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOI60961.1"
                     /translation="MAFFDRFRANVERLQQERDYAGLARALSAEDPSTRADAARALAA
                     LGVPAIPALLDALESARPASRNRMTESLVSTGIPSIPLLLAVILRASPALQASISRAV
                     AGAGDGMFEALLPALHHEQPAIRRAAVITLRDTGKKAVPHFAKMLHGKDPSVGKEAAN
                     ALAALHWAPDDPGEKAWFCYLREDWKELAKLQGAAVPILLKALCSNRARERCDAARTL
                     GKIRDGRTIPALIGAMKDPDIDLRIHAAEALGEMGDDRAKPALANALNDPHHRVRMEA
                     AWALNRLGWVPPNDLARAEHLVAGEQWNELVRMGRPAIPALIRALEVEYSGVRTGASE
                     ALRQLGQPALTALKTEIVSKNPARQQRARMALEYIRRRQEEASRTGPAPADSSRYEQE
                     LKEGLAIQRRFVKQFGRERPLPSVPVKKPQPKEEEAPAPPVPESTPVQASEEPKKPVV
                     LEDLIMESQQAEEAWARVKAQFKQEVSVPAGAIPLEQLIPLEFEEAIAGSDSAGETNE
                     EPARKEPGPRELDIPDIPQKAPKPDIDEPVPEKTPLEQYLEALKSSDESIRATAIAAL
                     QAMGKEAIVYLIAALKDPHYSVRIAAVEALGELKDDAAVDALILVCADIREDVRIAVA
                     RALGSIGDRRAIRPLIRLFGDRYHGVRVAAADAVAVFGRDALGPLEDALDDPAPPVRV
                     TAAKAIGLIGAGESVPALIEHLGDAAPEVRWSVARALAGFGPLAVEPLFLVLRKGKKE
                     MRLAAIDALWEVPGERADEALLYASEDEDGDVREKAAAALRKRQVINVWRMALGSQIR
                     MGESTQKKRSARFEDTAAFEASGQQEIDTLIVALKDKAWNAQLGAATRLIMMGRPAVD
                     GLIRALRNENPDVQTAAASLLGEMRETAVAPLMDALNDTERFVRLVAARNLGKIGNKR
                     AIEALIGSLHREPDSEVRATVAEALGYMGSRQAIEPLVLALRDRDEAVKIAAARSLGY
                     MGDTRALEPLILALADPDDRVRHIALDILKDPDETVRGCLIGALRSGDEVLRAGAAEA
                     LSAGGWVPGTGEEKALHLMARDQWADVERIGADALPAVAGALANPSVEVRINAVGTIA
                     RIGGDAAIAPLIRALRDDALAVRAKAGQALVDMGDAALPALAAEERRPEVRQIVDEIR
                     RGT"
     gene            complement(6918..7586)
                     /locus_tag="PLI76_02665"
     CDS             complement(6918..7586)
                     /locus_tag="PLI76_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOI60962.1"
                     /translation="MKILGINGSPRGSKSQTLRLVQAVLDGARSAGADVEVVDVCKLH
                     IEYCNGCTVCYERGECVKDDDFAALYEKMCESDGIVLGSPNYIDSVTAQIKTMLDRMA
                     DAIHCQMFIGKYGCAVSTAGGSGADDVVGYLNGILQTLGANTVGGVGVVFAGDPEAIV
                     PAEGRAYELGKRLVRAIEKKETYPEQEERHAAMLERMRTLILANKDRWHHEYDHWKAA
                     GRLP"
     gene            complement(7601..7993)
                     /locus_tag="PLI76_02670"
     CDS             complement(7601..7993)
                     /locus_tag="PLI76_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HOI60963.1"
                     /translation="MAGCTGTPGVPGTGVPGPEETVAVPATHSPPGPQPSFSTTVTAP
                     EKRFRADMSCYWIVTGTVTNTGDAPGRNVVIRFMLIDDGSAMIRSTETILIPRFPAGE
                     TKIFTANPLPGDCDRQYRADIVVMHDIP"
     gene            8192..8656
                     /gene="msrB"
                     /locus_tag="PLI76_02675"
     CDS             8192..8656
                     /gene="msrB"
                     /locus_tag="PLI76_02675"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HOI60964.1"
                     /translation="MSEKSAGTVKVCNAVTGKVEEVEKVVKSDEEWQRQLTPEEFHVA
                     RQGGTEPAFTGRYVDCKEDGLYVCVCCANHLFPSAAKFGSGTGWPSFFRPVSDLNIRT
                     AIDTRFFMTRTEVLCRRCDAHLGHVFDDGPPPTYERYCMNSASLRFVRKKDL"
     gene            complement(8738..9778)
                     /locus_tag="PLI76_02680"
     CDS             complement(8738..9778)
                     /locus_tag="PLI76_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60965.1"
                     /translation="MAPGGSHRNILLAVGLIGVILSIALLVPEACATAIPSLPEENAT
                     SGNASSFEAGPEDETLLPEPASDDERLESLIAGAGIPLLDVSVGQICAAYAGDDAALR
                     SRAADMASLVDGYRADAAALDISPDMAPAYEHFVAALDEFAAAAALDRSEDMPGRLAL
                     GTEHLSSALRACNPPASADAPPLLMSAGEEEPAPRFPDALGMGERFCYDDSRKENTIS
                     LIASSLTWSYAFQTTGTSPVRYEAAPGSYFLLVAVKATHLGHKGEGVNTRIQTPAESA
                     FTLLYGDATYRPLAPPGSTNRGGSYSRVTLGRGESLSGYLFFEVPEELDLSRAYLRAN
                     LGRESPVWLLDG"
     gene            complement(9827..10993)
                     /locus_tag="PLI76_02685"
     CDS             complement(9827..10993)
                     /locus_tag="PLI76_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60966.1"
                     /translation="MIVMDLQAPAVAILALALFTGILFSPSMAASEIADIEPSQPAAA
                     RNISTLLSGVEERLQGGRLLNFTPGQVADEPVENETPPPPVEEILEPVYDDDELDLLV
                     EENGISLMLLSLQNTCALFAWDEVPAKESGAALRALAAGLRRDVAAMDISEDREALKD
                     AFTRALESYEAAGTLLQGNAPLNRTTVDAAVEANLEGSDCLREASKYLERPLLKAPED
                     IAAISLSLPRPRTASASDEELMLLQRYVYEDRNRANDISLMFESVSKTRIYYARDEGG
                     EEVFAEPGRVFLLVKVKVTNLGHKGESRTYTVRTPDIRDFTLEYRKTTYTPIKFASGT
                     SLGVPYATATLGRYEVKAGYIVYDIPEALPLDECFVRVNLGQATSPVWAIGKMM"
     gene            11066..11698
                     /locus_tag="PLI76_02690"
     CDS             11066..11698
                     /locus_tag="PLI76_02690"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOI60967.1"
                     /translation="MCSVTDHAAAWDEDYRRRGNLWGGAVAPLPDIPAGAAVLELGCG
                     NGKTLSALARHSSGAVAIDVSPRAVALARQRPDTASVHLAVADARHLPFCGETFDAVF
                     IVHVAGHLTGPGRRALASEVRRVLLPGGTTFFRGFSIEDMRAGKGEETEHRTFARGDG
                     ILTHYFTEDEVSDLFTPLLPVLVRTHRWRMRVRGRDLPRAEIEAVFQRSA"
     gene            11881..12108
                     /gene="hypC"
                     /locus_tag="PLI76_02695"
     CDS             11881..12108
                     /gene="hypC"
                     /locus_tag="PLI76_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HOI60968.1"
                     /translation="MCIAMPAEVLEIKEGNIGVVDFGDLQQEVRLDLVDVSVGEFVLV
                     HVGFAIQRLSREEGLETREIFRQVHAAMMEE"
     gene            12108..12449
                     /locus_tag="PLI76_02700"
     CDS             12108..12449
                     /locus_tag="PLI76_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HOI60969.1"
                     /translation="MHEYSIAYDIYTTARRAAIENNAKEITCISVDVGRMAMVNPEQV
                     EFLFNVIIEEDPLFSGARLVCRDIEARTRCSCGYEGRERFVCPQCGRLPEVVAGMEIV
                     VTNIEIEVDEA"
     gene            12446..13441
                     /gene="hypE"
                     /locus_tag="PLI76_02705"
     CDS             12446..13441
                     /gene="hypE"
                     /locus_tag="PLI76_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HOI60970.1"
                     /translation="MKVNLMHGAGGEVMGELLQIITNLKHTNAGGIGLESLDDGAVIP
                     MCGQNVVFTTDSHVVSPIFFPGGDIGRIAVCGTVNDLAMMGGRPIALSCGMVIPEGFE
                     VADLERIVASMDIALGECGANLVTGDTKVLERGALDTIIINTAGIGVADRVVRDNGLL
                     PGDAIVVSGTIGDHGIAIMAHREGFDFGGQIRSDVAPLWMLVERALAAGDIHAMKDPT
                     RGGFANAINEMAKKSGVGVVIEEEALPFKKNVKSAAGMLGIDPLEVANEGKVVMGVAP
                     GDAEAVLAALRSHPYGRDAAIVGKVVEGSHVVMRTAIGGERFIEPPIGDPVPRVC"
     gene            13521..13952
                     /locus_tag="PLI76_02710"
     CDS             13521..13952
                     /locus_tag="PLI76_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOI60971.1"
                     /translation="MIEQRAIDVRLVGVWDGDMIADLYRAGGWWNEEWDPAGLRALIA
                     GSFAFAVAIDPAAKKAVGMGRAISDGVSDAYIQDLVVLPGYRGRGIGTMILSTLLDHC
                     KSAGVTWVALVAEPGTEAFYTPLGFRRMEGHTPMRWYPDER"
     gene            13957..14871
                     /locus_tag="PLI76_02715"
     CDS             13957..14871
                     /locus_tag="PLI76_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HOI60972.1"
                     /translation="MLEFSEFKPVSLDDRDLFLRQYRQYPQVHSDNTFANMVCWNHYA
                     NYCFADVDGAIVLSSTIDGVTSFRPPIGPKNPALLDDVIDLALREGGAAPLLVLDPVN
                     EAWIREIYPNLTLYPDRDYFDYIYRTEDLADFAGKGYATVRRQVNHFRREYAYSVEAI
                     TRENIDEVWEFLVIWCEWRDCDSVPILAYEKEAILFAINNFFAIGLRGWIIRVGETIG
                     AISVVDEVNADMAVVHFEKALPETYRDIYKVIMTETAAGLRGRYRFINRECDMGVPGL
                     RESKTRCHPACMVEVHYATRDDLEACRR"
     gene            14859..16121
                     /gene="pyrC"
                     /locus_tag="PLI76_02720"
     CDS             14859..16121
                     /gene="pyrC"
                     /locus_tag="PLI76_02720"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOI60973.1"
                     /translation="MPPVTADLVLKNVLLPTGRSADIVIAGGVVRHVGGAVRADETID
                     CSGLTCLPAAVDMHVHMRGGVQAEKEDWRTGTESAVAGGVTVVVDQPNTIPPLTTPDL
                     LCARIREAEEHAVCGFAVNAGVTTDADLEGMRNAGAMAFGEIFAAPSSYGEGLDPETL
                     ERLLARIHAMGGLATVHAEEVAGPAPATLAEHDRARSGAGEARAVRAVRALAPGGMRL
                     HFCHMSTAASVEAARGTVEATPHHLFLSRDMFAEDDARARVNPPLRDEETRRGLWSCW
                     ERIDIVASDHAPHTLREKELPFEGAPSGMPGVETMVPLLMAAVRRGDIPLASVIEKTS
                     WRPAAILGIRRAGFLPGDRADFALYPDAVTRIDAGSLHAKCGWSPFEGLDAIFPEEVV
                     VRGRRTYAGGELLEAHPAWYPGQGYLSL"
     gene            16320..16637
                     /locus_tag="PLI76_02725"
     CDS             16320..16637
                     /locus_tag="PLI76_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HOI60974.1"
                     /translation="MEPYADAVVETAHGVVITLDVTAGAKRSSFPAGYNEWRKSIRCQ
                     VAAPAVGGKANRAVVDLVAGTLGVPRADVVILSGHTSSSKTVAVAGRSRDGVLACLNA
                     AGA"
     gene            16880..18109
                     /gene="dnaG"
                     /locus_tag="PLI76_02730"
     CDS             16880..18109
                     /gene="dnaG"
                     /locus_tag="PLI76_02730"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase DnaG"
                     /protein_id="HOI60975.1"
                     /translation="MYSPETTKYLIHLTLQAEGVVDKPDVVGAIFGQTEGLLGEDLDL
                     RDLQRTGRVGRIDVQITTKRGETKGEILISSSLDRAETALLASSLETIDRVGPCTARV
                     KVDRIEDIRVTKRRKIVERAKELLLEDFDEGAISSDDLLDEVREVIRIEKLEYLGEER
                     VHAGPNVMASDAIILVEGRADVINLLRYGIKNAVAVEGTNVPRVIIDLCSQKTATTLL
                     DGDRGGELILRELLQVAEIDFVAYSPRGKSVEEMSRKEIVKALRNKVPVEVVTDHIVD
                     EGAAAERLPPPAGPAEARPPAGAGEGSSGERPAAKEGSRRPPSTLGEHMADVRDRKIA
                     RFLSPDYTVLAESNAADVEGALQTLAGDVEGLVVDRIVDQKLLDQIGGRDLEFVAARD
                     FKGIVKRPLSIRLIKIG"
     gene            18173..18457
                     /locus_tag="PLI76_02735"
     CDS             18173..18457
                     /locus_tag="PLI76_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0058 family protein"
                     /protein_id="HOI60976.1"
                     /translation="MHKEELIALHGILTEIKDYFELMNPELKFSQYYALKIDPSQVHK
                     SKMEHKYAIFVLGTELANAMKDVEFSSSGRISARMKELAEKALKEIEYLQ"
     gene            complement(18527..19321)
                     /locus_tag="PLI76_02740"
     CDS             complement(18527..19321)
                     /locus_tag="PLI76_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOI60977.1"
                     /translation="MIHIVPKPTDTPDDNSTGAVIRELERAGARCRVIDLGTIDPLDS
                     GLENELIWVCGIRQDGHQFETLSVLALHNQVVNTPESIVTCASKVMTTALLLHDGIRT
                     PETAYMRSEAQAGAFLKRHGKVVYKPLYGFDGNGIRLVERPDDFGPGPWYLQEYVPND
                     RDFRVFVIGGEAVGAIARVSEGLMHNIHQGGIGSPVPIDEEMRAIAEASARAVDIDYC
                     GVDLLRDKEGYTVLEVNGTPNWHCMAAPIPRLLAAYLIERRRAMRG"
     gene            19417..19962
                     /locus_tag="PLI76_02745"
     CDS             19417..19962
                     /locus_tag="PLI76_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOI60978.1"
                     /translation="MRENRIGIIGCGNPFMGNDGAGILVMQLFEGRYPGVDAIDGGTG
                     GFGLIPLMEGYEKVVIVDAMTGIGNRIGDVVVFATPPSLDLPACSLHDIGIGDAVAVA
                     RELGCAAEVVIIGIEVGDIRAFHRGVDPAVEGGVRDAEKEVIRLLREWTDEPCGTLAG
                     SKPGGGSESPSPGQGSSRVRL"
     gene            20049..20684
                     /locus_tag="PLI76_02750"
     CDS             20049..20684
                     /locus_tag="PLI76_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60979.1"
                     /translation="MQLPRGRFHRLIKSTTSRVLIEEMASARFTGICTIVPGEKSAVL
                     VLNEGLVVLAEYGGIRGQHALDAVSNDESEAAAELNLLTPEQVQLAREFNQPCAIDAG
                     DAGREKRTSPTKPAPGGGRPLAGVAEPTAACSPEHRAEPTGEGHRIPMPGTRPVQEMV
                     AATPSGNDEIDMLIQNMEKMDVEQLISNFRINCKEMLRNIHLDHLIQDGDI"
     gene            20707..21207
                     /locus_tag="PLI76_02755"
     CDS             20707..21207
                     /locus_tag="PLI76_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60980.1"
                     /translation="MDSTGIIQIFLVIMTVTVIAGFAGVLYYLSRIAQALAKEIPGGA
                     IESQERPAGNRESPGDIDPRGAKSLEENLGAISRKYSLASFTLATADGLLVGSTKTGS
                     EGEAARYSHLYARGERQDGTGAELLGIPYRGETLVGIARPKEHLSAELMNALEQDTRD
                     ALQRWV"
     gene            21244..>21447
                     /locus_tag="PLI76_02760"
     CDS             21244..>21447
                     /locus_tag="PLI76_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOI60981.1"
                     /translation="MVKVYTIASGKGGTGKTTVTANLGPMLARYGKKTCILDADVGMA
                     NLGLILGLEDLPVTLHEVLAGKAR"
BASE COUNT         4117 a         6733 c         7010 g         3587 t
ORIGIN      
        1 aggtctgcgg ggggcggctg cagccggtcc tcgacgcgac ggcgcttgca aaggaactcg
       61 gcatgcacgt cgagacggtc acgctggtca taccgggaca gaacgactcg atggacgaga
      121 tggagatgct catacgctgg gtgctcgata acctcggccc cgacacaccg atgcacttca
      181 cccggttcca ccccgactac aggatgctgg acgcaagacc gacagcggtc aggacacttg
      241 agaagatcta cgagcgtgca aaagagctcg ggctcaacta cccctacctc ggcaacgtcg
      301 ggggccaccc ttacgagaac acctactgcc ccgcctgcgg cagcctcgcc atagagcgat
      361 cgggctacgc agtccgcata cgggggatcg acgaccatac ctgcacggag tgcggcgggc
      421 gtatcgagta tgtctcaaca gtcgactgaa cagaggaggt tccttgccga ccggatgctc
      481 gggacgctca cccgctacct ccggttcatg ggctacgaca cgatgagcgc aaacagcata
      541 gccccgggca tggcccggga ggacacgctc ctcctcgaga tcgccacacg ggacaaacgg
      601 cttctcctga cccgggaccg cgaactcgcg cggcggggcg gtgcgcaggc ggtctacatc
      661 gtatccgaag atatcatgga gcagattcgc cagatcgccg gcctcggcct catcgagccc
      721 cgggtccgta tgaaccgctg ctcgatctgc aacacgcatc tccggcccgc caccgctcgc
      781 gaggtccgcg aggcacgcga ggtacgcgag gaggaccggg agagagagtt ctcgtggtgc
      841 cccgtctgcc ggaaacttta ctggatgggc tcgcacggcg agcacatcga agagcgcctg
      901 agggagtcct tctccccctg actataaaaa ctcctcattt cagcccattt ttcaaaaaaa
      961 gccaaagata tatcaacttt gagcgggcat gtagcgcggc ggccggaggc cggccgaccc
     1021 ctgtaccagg agcggttccg ggcacccggt aagggagtcg gccgcccggt cctccggacc
     1081 acgtggtccg ggcaatttac taagaggaaa atcatgaaac acttaaaaat agcgtttttt
     1141 tgctgggaat cattgtatgc ggagagggtc ggcgggcttg caaacgcggc gaccaacctc
     1201 gcggagacgc tggcacagcg ccacgaggtc cacttcttca cccggggaga gggaagggat
     1261 gcagagataa acaacgtcca ctaccactac tgccggccgg cgggagaaga tatcgttcgt
     1321 tactgctccg acatgagcca aaagatgctc gaccggttcg gcgagttcga tcggccggcg
     1381 ttcgatctac tgcactttca cgactggcac gtggtcgacg cgctcgtcag gctccgggag
     1441 cgcgagaccg tattcaccta ccattcgacg gagttcggga ggagcgggaa caggtacagc
     1501 gacgagtggc tgttccccgc catatccggg gcggaacgtc gcggcgggga gatagcaaaa
     1561 cgcgtgacgg cggtctcgtc gaccctccgc cgggaggcca tgaacctcta cgacataccg
     1621 gactggaaga tcgacgtcgt ccagaacggc atcatacccg gccattaccg ggcggacgtc
     1681 gaccccgagg cggtgaaaca gcgctacggc tttgaccccg gcgccccgct gatcctcttc
     1741 gtcgggcggc ttatgtggca gaaaggcccc gacatgctgg tggacgcagc cccccgcctc
     1801 ctccaggagc atgccgacag ccagtttgcg ttcgtcggcg acgggcagat gcggcggggc
     1861 cttgaggcac aggcccggtc gctccccgtc cgcttcctcg ggcggctgga cgacgccgac
     1921 tacgtctcgc tcctcaacgc aagcgatatc gtcgccatac cgagcagaaa cgagccgttc
     1981 ggcctcgtgc tcctcgaggc gtggagcgcc ggcaggtgcg tcgtcgcctc cgacgtcggc
     2041 gggcttgccg agaacatcga gcacgggacc gacggggtca aggtgcggcc gcgcccggac
     2101 tcgatcgccc gggggctctc ccttgcgtcg gactcaccgg cgctctcgct cgcgatgggc
     2161 cggagagggt ttgacaaggt aatggaggag ttcccgtgga gccgtgttcg agagcggatg
     2221 gagaactcgt accggaaggc ccgcgaaacc ctcctctgct gattcacggc agcatgacgg
     2281 cggtaaggtg ctccatcagg cggaacgggt ccgacgtgac gacgggcgtc cccgcgtaga
     2341 gctccatccc cccgatatcc gacgtccggg agtgaaggtc gccccggtcg accacccacg
     2401 tcacgtaatg accctcctcg ccaagcggcg gcatatacac cgccgcgttg tagctctgca
     2461 cccctatgtt acggtaacac gcgagcactt tagcaagtgc cggggcgata tcgtgcggcg
     2521 actcggtgat gatcgcgacc tcgtgctctt ttctcggcgt gaggtatgcc atgacccggg
     2581 cgccccggta ggcaaagccg agaccgatgg cgacatgggc agagaagagg tcgtcgtaat
     2641 actcgcaccc gtagcggcac cggtactcct cccgggcctg ctcgagacgt gccggggcgg
     2701 cgtaggggcg gtgggtgagg aggacctggg catggttgtg cacgatgctc gcgcccgcgc
     2761 gccagaggta gttccagacg atgaagggat agactgcccg ggaatcggcg cggtgcgcct
     2821 cctggcacca ccggacggcc acgctgacca tatcggccac ctgcggctcc gtgacgacgt
     2881 aagggttctt ctcctttgct atgatgacgg cgtggaggta atcgtacctg gcgatgttgc
     2941 tcgccgttat ccagtggtcg ccgtcgatcc ggccgaacgt atcggccggc gtttcattga
     3001 gaggatctgc aaacggccct ccctccgcca gcgcctcgag gtcccgcgtc tccccggcgc
     3061 ggggcgtcag aaccgggcgg cgtgcccgga cggagttgaa gagcgtgctc tcgccggtcc
     3121 ggtcattagt cacccggacg atctgctgcc tctcgatatc gccgtacttc tcgcgcaccc
     3181 acgcctgcat ggcgtccgga aagaccaggt tgccgggctc gacgtaccag gagtagatcc
     3241 gggagacgag gtcgcccgta tccggcgcga gttcccgcac gatacccggc agccgggtga
     3301 tatcaagtct cacctctaac atagtcccgg agagagaggg cggtaccata taatccttgg
     3361 ccggcgcgga tgtggaaagc ttatccgttc cggatagaga ctatactcag atctcaggga
     3421 gaactctccg gatagaacgt ttcagctgac catttccagg gatacggcga catggcattc
     3481 tttgacaggt ttcgagcgaa cgttgagagg ctgcagcagg agagagatta tgccggcctc
     3541 gcccgagccc tctctgcgga agaccccagc acccgtgccg acgcggcgcg agcgctcgct
     3601 gccctcggcg tccccgccat accggcgctt ctcgatgcgc tcgagagcgc tcgccctgca
     3661 tcgcgtaacc ggatgacgga gagcctcgtc tcgaccggca tcccctccat tcccctgctc
     3721 ctcgccgtca tcctccgcgc aagccccgcc ctgcaggcat ccatctcccg cgccgtcgcc
     3781 ggtgcgggcg acggcatgtt cgaggcgctg ctccccgccc tgcaccacga gcagccggca
     3841 atccggcggg ctgcggtgat aactctccgg gataccggga agaaagcggt cccgcacttt
     3901 gcgaagatgc tccacggcaa agacccttcg gtcgggaaag aggccgcaaa tgcgcttgcc
     3961 gcactacact gggcgcctga cgaccccggc gagaaggcgt ggttctgcta cctccgggaa
     4021 gactggaagg agcttgcaaa actccagggc gcagccgtcc cgatcctcct caaggctctc
     4081 tgcagcaaca gagcccggga acgatgcgac gcggcgcgaa cgctcggcaa gatccgcgac
     4141 ggccggacca taccggcgct catcggggcg atgaaagacc cggatatcga tctccgcata
     4201 cacgccgccg aggccctcgg ggagatgggg gacgatcggg cgaaacctgc gcttgccaat
     4261 gcgctcaacg acccccacca ccgggtgcgg atggaggcgg catgggcgct caaccggctg
     4321 ggatgggtgc cgccaaacga tctggcaaga gcggagcacc tcgttgcagg cgagcaatgg
     4381 aacgagctcg tccggatggg gaggccggcc ataccggcgc tcatccgggc gctggaggtg
     4441 gagtactcgg gagtccgcac cggcgcaagc gaagcgctgc ggcaactggg acagcccgct
     4501 ctcaccgccc tgaagacgga gatcgtatca aagaaccctg cacggcaaca gcgggcgcgg
     4561 atggccctcg agtacatacg gcggcgccag gaggaggcgt cacggacagg gcctgcgccg
     4621 gcggactcat cgcggtatga gcaggaactc aaagaggggc ttgccatcca gagacggttc
     4681 gtcaagcagt tcgggcgcga gagacccctc ccgagcgtgc cggtcaaaaa accgcagccg
     4741 aaagaggagg aggcccccgc ccccccggtc ccggagagca ccccggtgca ggccagcgaa
     4801 gagccgaaga aacccgtagt cctcgaagac ctcatcatgg agagccagca ggcggaagag
     4861 gcgtgggcgc gggtcaaggc ccagttcaag caggaggtat cggtcccggc aggggcgata
     4921 ccgcttgagc aactcatccc gctcgagttt gaggaggcga tcgccggaag cgacagcgcc
     4981 ggtgagacga acgaggaacc tgcccgtaaa gagcccggac cgcgggaact cgatataccg
     5041 gatatccccc aaaaggcgcc gaagcccgat atcgatgagc cggtcccgga aaagacgccc
     5101 ctggagcaat acctcgaagc cctcaagagc agcgacgaga gcatcagggc aacggcgatt
     5161 gccgcgctgc aggcgatggg gaaggaggcg atcgtgtacc tcatcgcggc gctcaaagat
     5221 ccgcactaca gcgtccggat cgccgccgta gaggcgctcg gcgaactcaa agacgatgcc
     5281 gccgtcgatg cgctgatcct ggtctgtgcc gatatacgcg aagacgtgcg gatcgccgtc
     5341 gcccgcgccc tcgggtccat cggcgaccgc cgtgctatac ggccgctcat acgcctcttc
     5401 ggggacaggt accacggcgt tcgggttgcc gccgccgacg ccgtcgcggt cttcgggcgc
     5461 gatgcgctcg ggccgcttga ggatgccctc gacgatccgg ccccgcccgt ccgggtgacg
     5521 gcggcaaagg cgatagggct catcggcgcc ggcgagtcgg tcccggccct catcgagcac
     5581 ctgggagacg cggcgccgga agtccggtgg agcgttgccc gggcacttgc agggtttggc
     5641 cccctggcgg tggagccgct cttcctggtg ttgaggaagg gaaagaaaga gatgcgcctt
     5701 gcggcgatcg atgcgctctg ggaggtgccg ggcgagcggg cggacgaagc cctcctctac
     5761 gcgagcgagg atgaggatgg agacgtgagg gagaaagcag cggcggccct ccggaaacgg
     5821 caggtgatca acgtctggcg gatggcgctc ggcagccaga tccggatggg agagagcacc
     5881 caaaagaaga gatcggcccg gttcgaggat acagcagcgt ttgaagcgtc cggccagcag
     5941 gagatcgata cgctcatcgt ggcgctcaag gacaaggcct ggaacgcgca acttggcgcc
     6001 gccacgcgcc tcatcatgat gggccgccct gccgtggacg gcctcatccg tgcgctcagg
     6061 aacgagaacc ccgatgtcca gaccgctgcc gccagtctcc tcggggagat gcgcgagacc
     6121 gcggtcgcac cgctcatgga tgccctgaac gacaccgaac ggttcgtccg gctcgtcgcc
     6181 gcgagaaacc tcggtaagat agggaacaag cgggccatcg aggccctgat cggctccctg
     6241 caccgggaac ccgacagcga ggtcagggcg accgtggcag aggcgctcgg ctacatgggg
     6301 agccggcagg cgatagagcc gctggtcctg gcgttgcggg acagggatga ggcggtcaag
     6361 atcgccgccg cccggtcgct cggctacatg ggggataccc gcgccctcga accgttgatc
     6421 ctggcgcttg ccgatccgga cgaccgggtc cggcacatcg cgctcgatat actgaaggat
     6481 cccgatgaga cggtgcgggg ctgcctgatc ggtgcgctcc gctcggggga cgaggtactc
     6541 agggcggggg ctgccgaagc gctctccgcg ggcggttggg tgccggggac cggcgaagag
     6601 aaagcgctcc acctcatggc ccgggaccag tgggccgatg tggaacggat cggcgccgat
     6661 gcgctgccgg cggtggcagg agcgctcgca aacccatccg tcgaggtgcg gataaacgcc
     6721 gtcgggacca tcgcacggat aggaggagat gcggccatcg cccccctcat ccgggcgctc
     6781 agggacgacg ccctcgcggt cagggcaaag gccggacagg ccctggtcga tatgggggac
     6841 gcggcccttc ccgccctcgc ggccgaggag aggcggccgg aggtccggca gatcgtcgac
     6901 gagatccgcc gcggtactta gggcagccgc ccggctgcct tccagtgatc gtactcgtgg
     6961 tgccagcggt ctttgtttgc caggatcagg gtacgcatcc gctcgagcat cgcggcgtgg
     7021 cgttcttcct gctcgggata ggtctctttc ttctcgatag cccgcacaag cctcttgccg
     7081 agctcgtaag ccctcccctc cgccggcacg atcgcctcgg ggtctcctgc aaagaccacg
     7141 ccgacgccgc cgacggtgtt tgcgcccaga gtttgaagga tgccgtttaa gtagcccacg
     7201 acatcgtcgg cccccgagcc gcctgccgtg gagacagcgc acccgtactt cccgatgaac
     7261 atctggcagt ggatggcgtc ggccatccgg tcgagcatgg tcttgatctg ggcggtgacg
     7321 gagtcgatgt agttcggcga gccgaggacg atgccgtcgc tttcacacat cttctcgtac
     7381 agggcggcaa aatcgtcatc tttgacgcat tcgccccgct cgtagcagac cgtgcacccg
     7441 ttgcagtact cgatatgcag tttgcagacg tccacgacct cgacatcggc cccggcgctc
     7501 cgtgcgccgt cgaggaccgc ctgcacgagc cggagcgtct ggctctttga gccgcgggga
     7561 cttccgttaa tcccaagtat cttcatcatt tcacctctat ttacggtata tcgtgcatga
     7621 cgacgatatc ggcgcgatac tgccggtcac agtcgcccgg gagcgggttt gccgtgaaga
     7681 tcttcgtctc gcccgccggg aaccggggga tgaggatggt ctcggtcgac cggatcatgg
     7741 cgctcccgtc gtcgatgagc atgaaccgga tgacgacgtt tcttcccggc gcatcgccgg
     7801 tgttcgtcac cgttcccgtc acgatccagt agcaggacat atcggcgcgg aaacgcttct
     7861 ccggcgcggt gacggtggtg gagaacgacg gctgcggtcc ggggggcgaa tgggtggcgg
     7921 gaaccgccac cgtctcctcg ggccccggga cgccggtccc gggaaccccc ggcgtccccg
     7981 tgcagcctgc catgacggca gaaccgatga ggcaggtgac gagcaggaga tatagagatc
     8041 tcttcttcag gggagttcac cctcctgcat gatggttcat cgaccggagg ctatataact
     8101 gatgcggaac cgggaggcgc cgcgagccgc tcccgccccg gcggggaaac gatttatacc
     8161 tccggggggt aaggagccgg gaggagttct gatgagcgag aagtccgccg ggaccgtgaa
     8221 ggtctgcaac gccgtgaccg ggaaggtcga ggaggtcgaa aaagtcgtca aatcagacga
     8281 agagtggcag cggcaactca cgcccgaaga gttccacgtt gcccggcagg ggggcacgga
     8341 gccggccttc acgggaagat acgtggactg caaggaagac ggcctgtatg tctgcgtctg
     8401 ctgcgctaac cacctcttcc cgtcggcagc gaagttcggg tcggggacgg gctggccgag
     8461 ttttttcagg ccggtatcgg acctgaacat acggacggcc atcgacaccc ggttcttcat
     8521 gacccggacc gaggtgctct gcaggcggtg cgacgcccac ctgggccacg tctttgacga
     8581 cgggccgccg ccgacctacg agcgctactg catgaactcg gcgtcgctcc ggttcgtgcg
     8641 gaaaaaagac ctgtaggggc cgtcgccttt ccaatatggc cgcaagtgcc cggacaggcc
     8701 ttcgcgcgat ctcgagggtg tggacagtga aacggcttca cccgtcgaga agccagaccg
     8761 ggctctccct gccgaggttc gcccggaggt acgcacggga caggtcaagt tcctccggca
     8821 cctcgaagaa gaggtagccc gagagcgact cgccccggcc gagcgtgacc cgggagtagg
     8881 accccccccg gttggtcgag ccgggcggcg cgagcggccg gtaggtcgca tcgccgtaga
     8941 ggagggtaaa ggcgctctcc gcaggcgtct ggatgcgggt gttcacgcct tcccctttat
     9001 gaccgaggtg cgtcgccttc accgcgacca ggaggaagta actccccggg gccgcctcgt
     9061 agcgtaccgg gctcgtgccg gtggtctgga acgcgtacga ccaggtcagc gaactggcga
     9121 tcagcgatat ggtattctct ttgcgggaat cgtcatagca gaaccgctcg cccatcccga
     9181 gagcgtcggg gaaacggggt gcgggctcct cctcgccggc gctcatgagc agcgggggag
     9241 cgtcggcgct cgcggggggg ttgcaggccc tgagggcact ggagaggtgc tcggtcccga
     9301 gggcgaggcg gcccggcata tcctcgctcc ggtcgagagc ggcggccgcg gcgaactcgt
     9361 cgagcgcggc gacgaagtgc tcgtatgcag gcgccatgtc gggggatata tcaagggcgg
     9421 cagcatcggc acggtagccg tccacgagag aagccatatc ggctgcccgg ctccggaggg
     9481 ctgcgtcgtc gccggcatac gcggcgcaga tctgccctac ggagacgtcg aggagcggta
     9541 tgccggcccc ggctatcaac gactccagcc tctcgtcgtc gcttgccggc tcggggagaa
     9601 gcgtctcgtc ttccggtccg gcctcaaacg aggatgcgtt cccggaggta gcgttctcct
     9661 ccggcagaga ggggatggcc gtcgcgcatg cctccggaac gaggagggcg atcgagagga
     9721 tcaccccgat caggccgacc gcaagcagta tatttctatg agaacctccc ggtgccacag
     9781 actaacctat tgcgcaccga agggatattc gttgcgctgg agggggtcac atcatcttcc
     9841 cgatcgccca gacgggcgac gtcgcctggc cgaggttcac ccggacgaag cactcgtcga
     9901 gagggagtgc ctccggtata tcgtagacga tgtaccctgc cttcacctcg taccggccca
     9961 gcgtcgccgt agcatacggc acgccaagcg acgtcccgga cgcgaatttt atcggcgtgt
    10021 aggtcgtctt gcggtattcg agggtgaaat cccggatgtc gggggtccgc accgtatacg
    10081 tcctgctctc ccctttgtgg cccaggttcg taaccttcac cttgacgagc aggaagaccc
    10141 ttccgggttc cgcgaatact tcctctccgc cctcgtcgcg ggcgtagtat atgcgggtct
    10201 tgctcaccga ctcaaacatc agggatatgt cgtttgcgcg gttccggtct tcatagacgt
    10261 agcgctgcag gagcatcagt tcctcgtccg atgcggacgc ggtgcgcggg cggggcaggg
    10321 agaggctgat cgccgcgata tcctccggcg ccttcagcaa cgggcgctcc aggtatttcg
    10381 acgcctcgcg aaggcagtcc gacccttcca ggttcgcctc gactgccgca tccaccgtcg
    10441 tcctgttgag gggggcgttc ccctggagca gtgtgccggc ggcctcgtag gactcgagtg
    10501 cacgggtgaa tgcatccttc agggcctcgc ggtcctccga gatatccatc gccgccacat
    10561 ccctgcgaag gccggcggca agcgcccgga gcgccgcacc gctctccttt gccggcacct
    10621 cgtcccaggc aaagagggcg caggtgttct gcaacgaaag aagcatgagc gatatgccgt
    10681 tttcctcgac cagcaggtcc agttcgtcgt catcgtagac gggctcgagg atctcctcga
    10741 ccggcggggg aggggtctcg ttctccaccg gttcatccgc cacctggccg ggggtgaagt
    10801 tcaggagacg gccgccctgg agccgctcct caaccccgga caggagggtt gatatgttcc
    10861 gggcggcagc aggctgcgag ggctcgatat cggcaatctc cgatgctgcc atggaagggc
    10921 tgaacagtat gcccgtgaag agagcgaggg ccagtatcgc cacagcggga gcctggaggt
    10981 ccataacgat catatctcac ctctcctgag acaaaaacct tccccgggca ggcggaacga
    11041 accattatgt ccgggcgacg ccgacatgtg cagcgtgacc gatcacgcgg ccgcatggga
    11101 cgaggactac cggaggcggg gaaacctctg gggcggagcg gtggcgccgc tccccgatat
    11161 acccgccggc gccgccgtcc tggaacttgg ctgcggcaac ggcaagaccc tctccgccct
    11221 tgcccggcat tcatccggag ccgtcgcgat cgacgtatcc cctcgcgccg tcgccctcgc
    11281 ccggcagcgg ccggatacgg cgtcggtcca tctggccgtt gccgatgccc ggcacctccc
    11341 gttttgcggg gagacgttcg acgcggtctt catcgtccac gtagccggtc acctgacggg
    11401 tccgggccgg agagccctgg catccgaggt ccgtcgggtg ctcctccccg gcggcacgac
    11461 cttcttccgg gggttctcca tcgaggatat gcgtgccggg aagggggagg agacggaaca
    11521 ccggactttt gcgagagggg acggcatcct cacccactac ttcaccgaag acgaggtgag
    11581 cgacctcttt acaccgctct tgcccgtcct ggtccggacg caccgctggc ggatgcgggt
    11641 caggggccgc gacctccccc gtgcggagat cgaggcggtg tttcagagat cggcatgaag
    11701 cggagtgccg cacttccccg gtcggacata cttttttaca cttccagacc gaatactacg
    11761 tagcagtata tacgggcccg ggggcgcacg ccggctaaaa cggcccatcc ccgggccctt
    11821 tgtgcagcaa ggtcaacctt ttttgaagcc cggacaaact aacagggagg aagagagttc
    11881 atgtgtatcg caatgcccgc tgaggtgctg gagataaagg agggcaacat cggcgtcgtc
    11941 gacttcggcg acctgcagca ggaggtcagg ctcgatctcg tggacgtgag cgtcggggag
    12001 ttcgtcctcg tccacgtcgg gtttgccatc cagcgtctca gcagagaaga ggggcttgag
    12061 acccgcgaga tcttcaggca ggtccacgcc gcgatgatgg aggagtaatg cacgagtaca
    12121 gcatcgccta cgacatctac acgaccgccc gccgggcggc gatcgagaac aacgcaaaag
    12181 agattacgtg catctcggtc gacgtcggca ggatggcgat ggtgaacccc gagcaggtcg
    12241 agtttctctt caacgtcatc atcgaggaag accccctctt ctcgggcgcc cggctcgtat
    12301 gccgggatat cgaggcgcgc acgcgctgtt cctgcggtta cgaggggaga gagcggttcg
    12361 tctgccccca atgcggaagg ctcccggagg tcgtcgcagg catggagatc gtcgttacca
    12421 acatagagat agaggtggac gaagcatgaa agtcaacctc atgcacggcg ccggcggcga
    12481 ggtgatgggc gagctcctgc agatcatcac caacctcaag cacaccaacg ccggcggtat
    12541 agggctcgaa tcgctcgacg acggggcggt catccccatg tgcggccaga acgtcgtttt
    12601 cacgaccgac agccacgtcg tgtcgccgat attcttcccc ggcggcgata taggacgcat
    12661 cgccgtctgc gggacggtca acgacctcgc catgatgggg gggcggccga tcgcgctctc
    12721 ctgcggcatg gtcatacccg aaggattcga ggtcgcggac ctcgagcgga tcgtcgcctc
    12781 gatggatatc gctctcggcg agtgcggggc aaacctcgtc acgggggaca ccaaggtcct
    12841 cgaacgcggc gcgctcgaca ccatcatcat caacacggcc ggcatcggcg ttgccgaccg
    12901 ggtggtgcgg gacaacggcc ttctgccggg cgacgcgatc gtcgtcagcg gcaccatcgg
    12961 cgaccacggc atcgccatca tggcccaccg ggaggggttc gacttcggcg gccagatccg
    13021 ctccgacgtc gcccccctct ggatgctcgt cgagcgggcg ctcgcggccg gcgatatcca
    13081 cgccatgaaa gacccgaccc ggggcgggtt tgcaaacgcc atcaacgaga tggcaaaaaa
    13141 gagcggcgtg ggcgtcgtca tcgaagagga ggccctcccc ttcaagaaga acgtcaaaag
    13201 cgccgccggc atgctcggca tcgatcccct cgaggtggca aacgagggaa aggtcgtcat
    13261 gggggtcgcg cccggcgatg cggaggccgt cctcgcggcg ctccggtcgc acccctacgg
    13321 gcgggacgcg gcgatcgtcg gcaaggtcgt cgaaggttcc cacgtggtca tgcggaccgc
    13381 tatcggcgga gagcggttca tagaaccacc gatcggcgac ccggtcccgc gcgtctgtta
    13441 gcgggcggtc acacctattt ttatgcccgc ccgatccacc atgcacatcg ttatgcggtc
    13501 gtatggagtg gtatgctgat atgatcgagc agagagcgat cgatgtgcgc ctggtgggcg
    13561 tctgggacgg ggatatgata gccgatctct accgggcggg cgggtggtgg aacgaggagt
    13621 gggaccccgc gggactccgt gctctcatcg ccgggagttt tgcctttgcc gtcgccatcg
    13681 acccggcggc gaaaaaagcg gtcggcatgg gcagggcgat atcggacggg gtctctgatg
    13741 cctacatcca ggacctggtc gtcctccccg ggtaccgggg ccggggcatc ggaacgatga
    13801 tcttatcaac gctgctcgat cactgtaaat ctgcaggtgt cacctgggtc gcccttgtcg
    13861 ccgaacccgg gaccgaagcg ttctatacac cgctcggttt ccggaggatg gaggggcaca
    13921 cgcccatgcg gtggtacccg gacgagaggt gaggcgttgc tggagttctc cgagttcaaa
    13981 cccgtgagcc tggacgaccg cgatctcttc ctgcggcagt accggcagta cccgcaggtg
    14041 cacagcgaca acacgtttgc caacatggtc tgctggaacc attatgcgaa ctactgcttt
    14101 gccgacgtgg acggcgcgat cgtcctctcg agcaccatcg acggcgtaac ctcgtttcgc
    14161 cccccgatcg gcccgaaaaa cccggccctg ctcgacgacg tcatcgacct cgccctgagg
    14221 gaggggggcg cagctcccct gctggtcctc gacccggtga atgaagcctg gatccgcgaa
    14281 atctacccga acctcaccct ttatcccgac cgcgactact ttgactacat ctacaggaca
    14341 gaagacctcg ccgactttgc cgggaagggc tatgccaccg tccggcgcca ggtcaaccac
    14401 ttcaggaggg agtacgccta ctcggtcgag gcgatcaccc gcgagaacat cgacgaggtc
    14461 tgggagttcc tggtcatctg gtgcgagtgg cgggactgcg actccgtgcc catccttgcc
    14521 tacgagaaag aggccatcct ctttgcgatc aacaacttct tcgcgatcgg gcttcggggc
    14581 tggatcatcc gggttggcga gaccatcggg gcgatatcgg tcgtcgacga ggtcaacgcg
    14641 gatatggcgg tcgtccactt cgagaaagcc ctccccgaga cctaccggga tatctacaag
    14701 gtgatcatga ccgagacggc ggcaggactc cgcgggcgct accgcttcat caaccgcgag
    14761 tgcgatatgg gcgtgccggg gctgcgcgag tcaaagacgc gctgccaccc cgcctgcatg
    14821 gtagaggtgc actacgcgac ccgggacgac ctggaggcgt gccgccggtg accgccgacc
    14881 tggtgctcaa aaacgtcctg ctcccgacgg ggcgctctgc cgatatcgtc atcgccggcg
    14941 gggtggtgcg gcacgtcggg ggagcggtgc gtgccgacga gacgatcgac tgcagcggcc
    15001 tgacctgtct ccccgcggcg gtcgatatgc acgttcacat gcggggaggg gtgcaggcag
    15061 agaaggagga ctggcggacg gggacggaga gcgccgtcgc cggcggggtg acggtggtgg
    15121 tcgaccagcc caacaccatc ccgcccctca ccacgcccga cctcctttgc gcacgcatcc
    15181 gcgaggcaga ggagcacgcc gtctgcgggt ttgccgtcaa cgcgggcgtc acaacggatg
    15241 cagacctgga gggaatgcgt aacgccggtg cgatggcgtt cggcgagata ttcgccgccc
    15301 cctcaagtta cggcgaaggg cttgacccgg agaccctcga aagactcctt gcccgcatcc
    15361 acgccatggg cgggcttgcc acagtccacg ccgaggaggt cgcaggcccg gcgccggcga
    15421 cgcttgcgga gcacgaccgt gcccgctccg gcgccgggga ggcgcgcgcc gtcagggcgg
    15481 tccgtgctct cgcccccggc ggcatgcggc tccacttctg ccacatgagc accgccgcct
    15541 cggtcgaggc cgcacggggt acggtggagg cgacgccgca ccacctcttc ctctcgcgcg
    15601 atatgttcgc ggaggacgac gcccgggccc gggtgaaccc cccgctcagg gatgaagaga
    15661 cccggcgcgg cctctggtcc tgctgggaga ggatcgacat cgtcgcctcg gaccacgccc
    15721 cgcacacgct ccgcgagaaa gagctgccgt ttgagggtgc cccctccggt atgcccggcg
    15781 tcgagacgat ggtgccgctt ctcatggcgg cggtgcggag gggcgacata ccccttgcct
    15841 cggtcatcga gaagacgtcg tggcggcctg ccgccatact cggcatacgc cgcgccgggt
    15901 tcctccccgg cgaccgggcg gacttcgccc tctaccccga tgcggtcacc cgcatcgatg
    15961 cagggagtct ccacgcaaaa tgcggttggt cgccgttcga agggctcgat gccatcttcc
    16021 ccgaggaggt cgtcgtccgg ggcaggcgca cctacgccgg cggcgaactc ctggaggcgc
    16081 acccggcgtg gtaccccggg caaggatatt tatcattata gatagaataa tatagagccg
    16141 atacagcgca ccttataggt ccccgggtga tcaccggcag aagcccgata tgatccatgg
    16201 gacaacccgg gtgcgcgaca ggcacgcccg gcatataggg tacgatcggg tgaggaccgg
    16261 cacaagccgc ctgtgatccg cgatcgttag tgccgggcga catatcacga ggctctttta
    16321 tggaaccgta tgccgatgcc gtcgtcgaaa ccgcacatgg cgtggtcatc acgctcgacg
    16381 tcaccgcagg cgcaaaacgg tcgtcctttc cggcgggcta caacgaatgg cgcaagagca
    16441 tccgctgcca ggtcgcggcc cctgccgtcg gcgggaaggc gaaccgcgcc gtcgtcgacc
    16501 tcgtcgcagg gacgctcggc gtgccgcgtg ccgacgtcgt catcctatcc gggcacacgt
    16561 cctcatcaaa gaccgtcgcg gtcgccggac gatcgagaga cggtgtcctc gcctgcctca
    16621 acgccgccgg tgcatgatcc ggaggtatat cacgaggatt gagttgctat tatagcggag
    16681 gatatataac ccgccataaa ttcaaaagat gcctggataa gactcaaatt tggatttctc
    16741 ttttaaaaaa ccttatgttc aatcccccat ctgatttaag taattgcacc ttcgatatat
    16801 aattgatcct ctgcccgcgc ataccgtgac ccgcgcggca cgagggcgat catacttacc
    16861 aggaaggagg catgactcca tgtattcacc agaaaccaca aagtacctca ttcatcttac
    16921 tctgcaggcc gagggggtgg tcgacaagcc cgacgtggtg ggcgccatct tcggtcagac
    16981 cgagggtctc ctcggtgaag acctcgacct gcgcgatctc cagagaaccg gccgcgtcgg
    17041 caggatcgac gttcagataa ccacgaagag aggcgagacg aaaggcgaga tcctcatatc
    17101 gtcgtcgctt gaccgggcag agacggctct ccttgcctcg tcgctcgaga cgatcgaccg
    17161 ggtcgggcca tgcacggccc gcgtgaaggt cgaccgcatc gaggacatcc gggtgaccaa
    17221 gcggcgcaag atcgtcgagc gcgcaaaaga gctcctcctc gaagacttcg atgaaggcgc
    17281 cataagcagc gacgacctgc tcgacgaggt cagggaggtc atcaggatag agaaactcga
    17341 gtacctcggc gaggagaggg tgcacgcggg cccgaacgtc atggcctccg atgccatcat
    17401 cctcgtcgaa gggcgggccg acgtcatcaa cctcctgcgt tacggcatca agaacgcggt
    17461 tgcggtcgag gggaccaacg tcccgcgcgt catcatcgac ctctgctcgc agaagaccgc
    17521 aacgaccctg ctcgacgggg accggggcgg ggaactgatc ctccgtgagc tcctccaggt
    17581 cgccgagatc gacttcgtgg catacagccc gcgggggaag agcgtcgagg agatgagccg
    17641 caaggagatc gtcaaggcgc tccggaacaa agtgcccgtg gaggtggtca ccgatcacat
    17701 cgtcgacgaa ggagcggcgg cggaacggtt gcccccccct gccggccctg ccgaagcgcg
    17761 acctcctgcc ggtgcgggcg aggggagcag cggcgagcgg ccggcggcaa aagaggggag
    17821 ccggagacct ccgtcgactc tcggcgagca catggccgac gtccgggaca gaaagatcgc
    17881 acggttcctc tcccccgact acaccgttct ggcggagtcg aacgccgccg acgtcgaggg
    17941 agcgctccag acgctcgccg gcgacgtcga agggcttgtc gtcgaccgca tcgtcgacca
    18001 gaagctcctc gaccagatcg ggggaagaga cctcgagttc gtggccgccc gcgacttcaa
    18061 aggcatcgtg aagcgccctc tctccatcag gcttataaag atagggtgaa gattatccgg
    18121 ggtgcaaatt tatatatgtt tatatctact atttttatct ccgggagaga atatgcacaa
    18181 ggaagagtta atagccttac atgggatact tacggagatt aaagactatt ttgaattgat
    18241 gaatccggag ttgaagtttt cacagtatta cgcattaaag atagatcctt ctcaggtgca
    18301 taagagcaag atggaacata aatacgcaat attcgtgctc ggaacggaac tcgccaacgc
    18361 catgaaggac gtggagttct catcatccgg gaggatatct gcccggatga aggaactcgc
    18421 cgagaaggcc ttaaaagaga tcgagtatct ccagtaacga ctttcctgcc ccgcatcgcc
    18481 cgcgggcagg gtgcccggcc ggggccttgc cccggcgctc ctctcctcaa ccccgcatgg
    18541 cgcgcctgcg ctcgatgagg tacgccgcca gcagccgcgg gataggcgcc gccatgcagt
    18601 gccagttcgg cgtcccgttc acctcgagga cggtgtaacc ctccttatcg cggagaagat
    18661 cgacgccgca gtagtcgata tcgaccgccc gcgccgatgc ctcggctatg gcccgcatct
    18721 cctcgtcgat cgggaccggc gagccgatgc cgccctggtg gatgttgtgc atcaggccct
    18781 cggatacacg ggctatcgcg ccgacggcct ctccaccgat gacgaataca cggaagtccc
    18841 ggtcgtttgg cacgtactcc tgcagatacc agggccccgg cccgaagtcg tcgggtctct
    18901 ccaccaggcg gatgccgttg ccgtcaaacc cgtagagcgg tttatagacg acctttccgt
    18961 gccgcttgag gaacgccccg gcctgcgcct ccgagcgcat gtatgccgtc tccggcgtcc
    19021 ggatgccgtc atggaggaga agggcggtcg tcatcacctt ggatgcgcag gtgacgatgc
    19081 tctcgggcgt gttgaccacc tggttatgga gcgcaagcac gctcagggtc tcgaactgat
    19141 ggccgtcctg ccggatcccg cagacccata tgagttcgtt ctcaagcccg gagtcgagag
    19201 gatcgatcgt gccgagatcg atgaccctgc atcgggcacc ggccctctcc agctcccgta
    19261 tcaccgcgcc ggtcgagttg tcgtccggcg tatcggtcgg tttaggtacg atatggatca
    19321 tacgcctcac attccgctcc cgggactgac gatttgctcc ccgggtctca gtaccctttt
    19381 gtacctcgcc ggatataatc agtggggaag gcgaagatgc gagagaaccg gatcggcatc
    19441 atcgggtgcg gaaacccctt catggggaat gacggagcgg ggatcctggt gatgcagctc
    19501 tttgaaggga ggtacccggg cgtcgacgcg atcgacggcg gcaccggggg gttcggcctc
    19561 atacccctga tggaggggta cgagaaggtc gtgatcgtcg atgcgatgac gggcatcggc
    19621 aaccgtatcg gcgacgtcgt cgtctttgca acaccgccct ctctggacct tcccgcatgc
    19681 tcgctccacg atatcggcat cggcgacgca gtggcagtgg cccgagaact cgggtgcgcc
    19741 gcggaggtcg tgatcatcgg catcgaggtg ggcgacatac gggcgtttca ccggggcgta
    19801 gacccggcgg tcgaaggagg ggtccgggat gccgaaaagg aggtcatcag gctcctccgg
    19861 gagtggaccg atgagccttg cggcaccctt gccggcagca aacccggggg cggatctgag
    19921 agcccctctc cgggccaggg cagttcacga gtgcggctat gatgtgcagg ggcgcgagac
    19981 caccatgccc gtttcaaaaa gtataaatga caaggtttat ctcatacaac agtattgagg
    20041 atgtccgtat gcagctgcca cgaggtcggt tccatcgcct tatcaagtcc acgacatccc
    20101 gtgtcctgat agaggagatg gcttccgcac gattcaccgg gatctgtacg atcgtccctg
    20161 gcgagaagag tgccgtcctg gtgctgaatg aaggtctggt ggtgcttgcc gaatatgggg
    20221 gcataagagg acagcacgca ctggacgccg tatcgaacga tgagagcgaa gcagcggcag
    20281 aactgaacct cctgacaccc gagcaggtac agctcgccag ggagttcaac caaccgtgtg
    20341 cgattgatgc cggcgacgcc ggcagggaga aaagaacatc accgacaaaa cccgcaccgg
    20401 gcgggggaag accactcgct ggagtggcag aacccactgc ggcatgctca ccggagcacc
    20461 gcgcggaacc gacaggggaa gggcacagga tccccatgcc gggcacgaga cctgtgcagg
    20521 agatggttgc agcgactcca tccgggaacg acgagatcga tatgctcatc cagaacatgg
    20581 aaaagatgga tgtcgaacaa ctcatcagca acttccggat aaactgcaaa gagatgctca
    20641 ggaacattca tctcgaccac ctcatccagg acggagatat ctgaacagac aggggctgac
    20701 aacagaatgg attcaacagg gatcatccag atcttcctcg tcatcatgac ggtgacggtg
    20761 atagccggtt tcgccggcgt tctctactac ctctcaagga tcgctcaggc actcgccaaa
    20821 gagatcccgg gaggcgccat cgagtctcag gagagaccgg caggcaaccg ggagtcgccc
    20881 ggcgatatcg atcctcgagg tgcaaagagc ctggaggaga acctgggcgc gatcagcagg
    20941 aagtacagcc ttgcatcctt cacgctggca accgcggacg gcctcctggt cggttcgacg
    21001 aagacgggca gcgagggcga ggcagcccgg tacagtcacc tttacgcccg gggagagcgg
    21061 caggacggga ccggagcgga gctccttggt attccctacc gcggggagac gctcgtcggc
    21121 atcgcccgcc caaaagagca tctctctgcc gaactgatga acgcgctcga acaggatacc
    21181 cgcgatgccc tgcaacgctg ggtgtaagat cggcgggttc gatatacgat aacgagtgat
    21241 tcaatggtaa aagtatacac gattgcatcc ggcaaaggcg gtaccggcaa aacgacggtt
    21301 acggcaaacc tggggccgat gctggcaagg tatgggaaga agacatgcat cctcgatgcc
    21361 gacgtcggga tggcgaacct cggcctcatt ctcgggcttg aggatctgcc ggtgaccctg
    21421 cacgaggtgc tggcaggcaa agcccgc
//
LOCUS       DAOSEE010000020        20985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1089_length_20985_cov_250.851171, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000020 DAOSEE010000000
VERSION     DAOSEE010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 20985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20985
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1089_length_20985_cov_250.851171"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="PLI76_02765"
     CDS             <1..375
                     /locus_tag="PLI76_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60982.1"
                     /translation="AVMTRQADATGSAMLAEPRWFYDGPTKTFVVYLMNITADERMAK
                     SGMATVRMSLASTNSQQYDPVGDITVQYDKDAHNDLSVAWENYLTALGMLKTDPHKYE
                     WDINKPVEKLVIKEYKIRILGI"
     gene            complement(453..779)
                     /gene="eif1A"
                     /locus_tag="PLI76_02770"
     CDS             complement(453..779)
                     /gene="eif1A"
                     /locus_tag="PLI76_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor eIF-1A"
                     /protein_id="HOI60983.1"
                     /translation="MTDPSKRKPESNGGDAEGEIIRVRLPNKRNREMFGSAELMLGAN
                     HIRIRCFDGVTRTGRIKGKIKKKVWIREGDVLIVVPWSFQDEKCDIIYRYTRPQVEWL
                     RKNRYL"
     gene            1014..1670
                     /locus_tag="PLI76_02775"
     CDS             1014..1670
                     /locus_tag="PLI76_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60984.1"
                     /translation="MTCLDSSRGVYLTYFIQGLILLAAAASIMGGEYLSGLSAAFAFL
                     LTLAPSFATRNMRFCLPWGVNLLIAVSLYLHVMGYTGGYYAALAYYDKLAHLVSSATV
                     ALIVFSLMVLAGHRGDVRLTTPAVVASILIATLAAGALWEIYEFAVDQIFGTSLQLGN
                     TDTMWDLIMDLIGAAIVAGFAAFVLAREDRFTGIFAGLISVAEPADVMDNQADSAKSR
                     "
     gene            1771..2649
                     /locus_tag="PLI76_02780"
     CDS             1771..2649
                     /locus_tag="PLI76_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOI60985.1"
                     /translation="MLIMLPSMTGYMAWEAQIVDITTLEIDGAPEGVVYITDPHVGPS
                     SIDHIQKVIQEVNRLNPSLVLIGGDFATGDEENFTYQGVWGSLDAPAYAVLGNHDYRV
                     GTDGATGLERTIAVWTSTNTTTAGYDVSALNDGSADTAFADGLAATLEENGVYVLRND
                     YARVDAGGEEIVIVGVDDGWAGMADPPDVPATDAFTIYLIHEPSCRADWDADLILAGH
                     THGGQFLFPVVAQLNDIGVIELAGLFETNGTPTYISRGVSSAGIAGVGLRFNCRPEIV
                     LINPTEDQLRVLGNPA"
     gene            complement(2657..3487)
                     /locus_tag="PLI76_02785"
     CDS             complement(2657..3487)
                     /locus_tag="PLI76_02785"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HOI60986.1"
                     /translation="MKHVTKSDGNQQPFDRGKVRRTLQNLGLGAEDADRIADEIEESV
                     PDGMKTAAIFRMIRARARAVRPAVRHRADLRRALTLLRPKPDFEEFVRVLLREHGYQV
                     QTGCVLAGRCGEHEVDAIASKDGVTIFVEVKHHANHHRMTGLDEGRIARAIVEDLQEG
                     FRSGQCTVSIDGALIVCNTRFSEHAKRYAACRGIEHIGWDYPEGQNLKTMIEETQSYP
                     VTIVSGMSSSVSARLASAGILTAKQVAYGDATTIARDTALSLPEVLVIAGRARAILER
                     "
     gene            3590..4531
                     /locus_tag="PLI76_02790"
     CDS             3590..4531
                     /locus_tag="PLI76_02790"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HOI60987.1"
                     /translation="MDALQRPVVLILGAGAVGLSLAGRLLSVATVYAACRPAHAGAIR
                     KRGLVMEGIWGNTTHGGLACVTGPDEVPPAVDFVIITAKGTDTRAICEEYAGQLRDRP
                     VASLQNGIGNEEIIARYAGTVIGGTVTTNFSVVGSGHVRVASESAPMRLGVWSGDERA
                     LDELIGIIRSAGIPVEADGDIRSGKWAKALLNIAVNPICALLAAPVGAAADEDVRETV
                     AGLIRETFAVAGAEGVHLPWASAGDYLAHLFTVQVPDFAAVYPSMYYDLQRGRRTEID
                     LLNGYVVRIGERHGIETPYNRCIAGLVRYAEAHPEPS"
     gene            complement(4536..4715)
                     /locus_tag="PLI76_02795"
     CDS             complement(4536..4715)
                     /locus_tag="PLI76_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60988.1"
                     /translation="MQEYGPGIVGEVRFARQDGGYYVQLYDRGGNPVGKTGLWRSEAK
                     AKEAARKLAAKIGEA"
     gene            complement(4763..5038)
                     /locus_tag="PLI76_02800"
     CDS             complement(4763..5038)
                     /locus_tag="PLI76_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HOI60989.1"
                     /translation="MSLPIAFLALVLVVVVAFVLYRTVKSVTGLIINAVVGVILLWLI
                     NLLGLMHLVGRPDIPINLITVLICAVGGIFGVLVTVVLHLLGISLTL"
     gene            complement(5086..5640)
                     /locus_tag="PLI76_02805"
     CDS             complement(5086..5640)
                     /locus_tag="PLI76_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOI60990.1"
                     /translation="MVWSIIQAILLLSALIPQGHLICDGGWGGPEPFCIDDGAWFSIP
                     EEILSSESTDSSCTGFIEQVDRATFARVDGVPRLFPVGLNATLTRGAGDCTDLALLRV
                     EILRRNGIPARPVHGIMVWDAETFWTDALHMEVFGRGIAVHDWVELDDGQTMGAYEGI
                     PGIRCVKLGNGVCLQPVFEALGYM"
     gene            complement(5829..7061)
                     /locus_tag="PLI76_02810"
     CDS             complement(5829..7061)
                     /locus_tag="PLI76_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOI60991.1"
                     /translation="MSDTIAIIGGGPAGLFCALQAAGRGGRVILLEKKPAPARKLLIT
                     GSGQCNITHDGEVTGFLSHYGDHGAFLRPALRNFTNRDLIAFFADRGLPMQAEPGGKV
                     FPETRRAADVLAVLLSECSARGVVIRCGEPVVAVEREGDGFLVRTEKESVRAGVLVIA
                     TGGASYPATGSTGDGYAFARALGQPVTEVAPALTPVSIEGYPFADLAGISFENAAFAI
                     HRGGRVVRRHTGDILLTHAGLSGPGILDASRFILPGDVLRVTFLCGANAEAVRKRVAG
                     ALREGGARQAKTILCDLPLPERFARRLLDIAGVPAGATCAHLPRKARDALAASVAEFP
                     FTVARLGGFHEAMATRGGIALDGIDPKTMVSRVVRNLYCIGEVLDIDGDTGGYNLQAA
                     FSTAALAARDIAGHPISR"
     gene            7177..8193
                     /locus_tag="PLI76_02815"
     CDS             7177..8193
                     /locus_tag="PLI76_02815"
                     /inference="COORDINATES: protein motif:HMM:NF021607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HOI60992.1"
                     /translation="MNDTWILLMGGVLLVLLITPGSAASEGAPGTGGFAGRQAIAIDH
                     PSGTEGLEVLFNATYLPGMRPDFADIRFLDGNETAIPHWTATVMEGSHAHVWLALPAN
                     ATTITMLYGNPAARDAGDPDAVFLFYEDYERGDLSRWSFCGSNAAVRSDVVRSGGYAG
                     DINVSEPDLRELANNRICLANISAGPIIVEGDFLVSKFGKWCGSGYIQAWSGPDRLYA
                     LHLRNGSVQYYDGAHRNFSADARAEEETWYHIKVALDTPNATGHAWMDGRYLGKMTLR
                     FADGSPVPEETIFTDIALIGSSAWGSGDPHHFCADNVVVRNYVPVEPVLSYEVNAPGC
                     TGDP"
     gene            complement(8333..8908)
                     /locus_tag="PLI76_02820"
     CDS             complement(8333..8908)
                     /locus_tag="PLI76_02820"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOI60993.1"
                     /translation="MIEERLRARTSGILVGAATLVARTGVTPNTLTVLGFVGMAVAGA
                     LCAAGSFFFAGVVVAASCIFDALDGALARASGTASVFGAFLDSFLDRYAEAAVYAGLL
                     VHYAGSGSPSGAVAAFLAAIGSLMVSYARARAEGLGIECRAGLFARPERLAVIIAGLV
                     TGLVLPALVILSVATNATAVRRLLHVLQSTG"
     gene            complement(9088..10155)
                     /locus_tag="PLI76_02825"
     CDS             complement(9088..10155)
                     /locus_tag="PLI76_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60994.1"
                     /translation="MVLEEMIIIRGPIDGPEIARAFREAGMPTRITQTVTLDGRVAAK
                     GTIGDIRALLKGEIARIEKLRKEFLADEEGEEFDAPEDKEGADDREDKEGADDREDEE
                     DADDWEDEYFDTGHLYDPYLDMTECIAEDIARFMEKYRPGDVISMADLKEWMAPLADV
                     APGEERSDALAKSILYKMTALSMLDKNGLIEIVGETVTVKGHMDPEDVIITIPGEAAE
                     EIIPETLKEHNVTMAVTVTPVPEHLLIFGPEAIIDGDLDTIDEIIDDLGIDEEEYISF
                     RAGVSMKRAVIARTLEILEERGTLTPEEALETLNGTPLEGTEEAYSISLELTSEFVKG
                     LLNDLKKVGLVRKKGEKFRAI"
     gene            complement(10186..11361)
                     /locus_tag="PLI76_02830"
     CDS             complement(10186..11361)
                     /locus_tag="PLI76_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOI60995.1"
                     /translation="MAGVKVYTTESCPYCRMVQAFLQKHGVEYTIVDVGKEREAAREM
                     VRISGQRGVPVTVFGDEVIVGFDAKKLRELFGTPVADEIYDAVIVGAGPAGLTAAVYC
                     ARKLMKTIVVSEDIGGQAAWSWAIENYMGFRMITGEDLIGKFEDQVRGFDVGLELDRV
                     RGIQKEGDLFFVRASSTYRCRTVILAPGKQPRTLGLPGEDRLIGRGISVCSTCDGPLF
                     RDRPIAIVGGGNAAIQTAVEMTEIASSVALIVRSALRCDEIYIRRAEEAGVRVFPNHE
                     VVELHGDTALAGVTVRDRETGRETALDVEGLFLEIGRIPNTGFLGDLVAQNEQHEILV
                     DENNHTNVDGIFAAGDATCIKGKQIIIAAGEGAKAALEAHEYLIKKGLSRAPLSATL"
     gene            complement(11452..11952)
                     /locus_tag="PLI76_02835"
     CDS             complement(11452..11952)
                     /locus_tag="PLI76_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOI60996.1"
                     /translation="MPTDENAQEAFAGESQANRKYSVFAEKATAEGYPVVGKLFRAAS
                     EAEAVHAKRLLFIKNAVGSTEENLKGAIEGENYEFMTMYPTFAEEAKKERRNEAAIVF
                     THAMKAEEVHANLYLQALEAVREGKDIDVEKIYLCPVCGNVELGAAPEKCPICGVPAR
                     MFREVQ"
     gene            12041..12907
                     /locus_tag="PLI76_02840"
     CDS             12041..12907
                     /locus_tag="PLI76_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOI60997.1"
                     /translation="MQGDRVQRVFVIVLILNVAVAVVKAVFGVMAGSVSMVADALHSG
                     FDSFSNVVGIVAMHLAGKPPDPEHPYGHGKIETLGTLVIGAMLLLTAAGILLEGYGRL
                     ITPVTPEITPITVGVMVGTLITNIIVSTYERRKGEEYQSQILIADSLHTRSDVFVSTA
                     VLGGFLAIRLGYPAADPIIALGIGLLIARMGVGILYGAAEVLIDSMNLPCDPALVRAV
                     VMDTPGVAGYHDFRCRGKPGEIFADIHVTVDPALSIARAHAISEEVERRLKETVPGLA
                     EVVVHIEPDDSA"
     gene            complement(12899..13498)
                     /locus_tag="PLI76_02845"
     CDS             complement(12899..13498)
                     /locus_tag="PLI76_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOI60998.1"
                     /translation="MGRKVIALLGSPLPDGNTARLLDEAIRGAEEAGCDVERIVAAHL
                     DIAPCMEIFYCQENETCEIRDEMIGIYDKFREMDGLIIATPVMTMGIPGRLKSFMDRF
                     QVFYMAKYHRGRPFISPEQRRRRKMLFISIAGMNLPTVFEGAKMTAKAFGEIVDCPYW
                     DEVLRNDMDTIQDIRTRPEVMEAAYRKGYELGQLLSSSR"
     gene            13626..13856
                     /locus_tag="PLI76_02850"
     CDS             13626..13856
                     /locus_tag="PLI76_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI60999.1"
                     /translation="MAEPGTRTTAKAGEKPGPGRFICIDCGKEVRIGSTDEDLVKCPT
                     CACEMYNCLPMTHIRPDIKTPEDVMNPPERKK"
     gene            13882..14007
                     /locus_tag="PLI76_02855"
     CDS             13882..14007
                     /locus_tag="PLI76_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin FeS4 iron-binding
                     domain-containing protein"
                     /protein_id="HOI61000.1"
                     /translation="MVNVSAKGEIYRCEICGNVVEVLEAGGGELICCGEPMVLEE"
     gene            14013..14369
                     /locus_tag="PLI76_02860"
     CDS             14013..14369
                     /locus_tag="PLI76_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOI61001.1"
                     /translation="MAEEQKNLEEKIGKIPEIFKELKETDPDLYGKVMGLDQMIWADG
                     ALSKQTKKVIAIAIAAALRDGHAVRAQMAGAGNLGVSKEEIEEGLRVAFLLAGMPAYV
                     HGKTALEEYLGSRPRK"
     gene            14374..15018
                     /locus_tag="PLI76_02865"
     CDS             14374..15018
                     /locus_tag="PLI76_02865"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOI61002.1"
                     /translation="MEPEGLPQMPVIGEKAPEFDALTTHGPLKLSDLAGKWVILFSHP
                     ADFTPVCTTEFIAFAGMADELAKLNVQLVGLSIDSVHSHLAWVRNIKEKMGVDIPFPI
                     IADLDMKVAKRYGMLHPGQSSTSTIRTVFFIDDKGIMRAMLYYPMSSGRSMPEILRLT
                     KALQTADKHHIATPANWEPGDKVIVPAPKTPKEIEKRMQEGYECKDWYLCFKKL"
     gene            15060..15671
                     /locus_tag="PLI76_02870"
     CDS             15060..15671
                     /locus_tag="PLI76_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOI61003.1"
                     /translation="MTIDVLAFATSPRRRGNSETLLDWMLDAMKEEGAAVEKIVVPEV
                     DIRPCRGCNACAKLNRCIQRDYMDYVHDRIVEADCIILASPIYCMGLTAQAKVLVDRA
                     QVFCTRKYVLRLPVVPPERKGKRVGVFLSTAGQNWDHVFDAAIPSVKCFFNVIDVRDK
                     DIRYLMVNNVDEKGAIACHPTARADAAALAGEVAAYLQEVGVA"
     gene            15668..16294
                     /locus_tag="PLI76_02875"
     CDS             15668..16294
                     /locus_tag="PLI76_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOI61004.1"
                     /translation="MTDPVTVLGISGSPRRHGNTETLLDAVLEGTREAGADVEKIVLR
                     SLAYTPCRGCNVCHKTGVCIIEDDLTEIFDKIAAADVLIIASPIYSMGITAETKGLID
                     RAQYLWARKFILKTLYFTTEQTRRHKGIFVSTAGLGWGNVFDAAFPAITAFFNTTGFE
                     YWDNVIANDLDRYGGIAGHPTALAAGREKGREVVALIRKMQAREGTET"
     gene            16529..19474
                     /locus_tag="PLI76_02880"
     CDS             16529..19474
                     /locus_tag="PLI76_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOI61005.1"
                     /translation="MPLELRLLDGIDDWLLLIGRDHNVIRVNSAIRRIHGALEGDLDQ
                     AVCALGGSRGLAGRIRTALDDEDEIPGFECRLQGPGGGERRYLCSGWRMPPGEGWLLR
                     FREIPVAGDYEAVVEHTGTATILIEDDGTISLANTEFERLSGYSRREIVGVKRLTDFI
                     ASEDECRQIREYHILRRSNPAAAPKNYSFSFIDRAENVHIVEATIGMMPGTARAVMSL
                     INVTGRRRAEQALQESEERLNLAFLAANDGLIDWDVRTGKIFYSPRSFTMLGYEPGAF
                     VPTYPMILTLVHPEDRDRVGEALADLTSGRRDRCDMEFRVRSASGDFVYVLDRLQVVS
                     HGAGGEPLRIVGTHSDITERKEAERELRIKDMAIASSLDAISLTDLEERITYANRAFL
                     TMHGWDREEIIGQPASVLWADPEEAKRVIAEFLRDGTWSGEVAGRRRDGSTVHMQLAL
                     STITDEAGARLGSMGSGIDITRRKQAERELLIRNMAIASSLTAFAITDLDGRLTYANQ
                     AFFSLWGYTSAAEVVGRRVTGFWQDEGEAEDAVRMLLETGRYTGERVGRRRDGAKFYA
                     MFSGNLVRDEAGAPICLAASLTDITDLKRAEAEIQAHNRELSVLNQIIGVSTSATGID
                     EALGDMLAATIALLDLRGGGVYLVEADRQRARLICTRGLPDGFPLQTCIPDITAPPYA
                     DVLVAGEPFFFDDPTHLRYPGEEREMLHPYASIPIVASRGIIGSLNVVPRDRRRFTDA
                     DRSLLLAIGKEVGSSLECILLIRQLESAEREANLYLDILSHDIRNAENISSLYIDILI
                     DMLEGDAKDHAQKIRSSIRKSIEILRNVSTIRRIHHESVTLAPVDLDEVIRDEIGVFS
                     EINFNYEGTDHAVMADPLLPEVFTNLIGNAVKFGGSAVEITIRVEESGNDVLISVEDT
                     GPGISDEMKDTVFMRFGKNRIQKSGQGLGLSIVRMLVERYGGTIRSDDRVSGCPGCGA
                     AFRFTLKRA"
     gene            complement(19490..20074)
                     /locus_tag="PLI76_02885"
     CDS             complement(19490..20074)
                     /locus_tag="PLI76_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M50"
                     /protein_id="HOI61006.1"
                     /translation="MLERIPLRERRDLLIAWLAISIAFTLIYIRGGVNVTGLIFFFVM
                     SLLTVGVAFILHELAHKFTAMRYGYWAEFKKDNQMLLVAVVMAALVGVVFAAPGATYI
                     YGNATRAENGRISAAGPLTNLLLCIPFALLMLFGGGLIGIVGLVGFRVNAMIATFNML
                     PISVLDGRKILAWNPAVFAVLMVASAGALFWSLL"
     gene            20176..20460
                     /locus_tag="PLI76_02890"
     CDS             20176..20460
                     /locus_tag="PLI76_02890"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HOI61007.1"
                     /translation="MSLDAVRSEIRGIDEKIIDLIAERQRLAARIARLKQEEGLPIRD
                     GPQRKAVLDRVFAHAAESRIDPVAVRNIFEILIDMNEERQRECSGDGNLP"
     gene            complement(20470..>20985)
                     /gene="argB"
                     /locus_tag="PLI76_02895"
     CDS             complement(20470..>20985)
                     /gene="argB"
                     /locus_tag="PLI76_02895"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOI61008.1"
                     /translation="SGNDGNLLIARKMEPQRVRVGEVEEEVDLGRVGEIEEVDPEVLH
                     CLLAQNYIPVVAPIAIDRQGGSLNINADTAAAEIAIALQAFKLINLTDVDGVMNADRT
                     MTYHRLTLSEADTMIAEGIISGGMIPKLDGCMRAVRSGVTSAHVVNGNREHNLLLELF
                     TDEGVGTMITL"
BASE COUNT         4043 a         6680 c         6360 g         3902 t
ORIGIN      
        1 gcggtgatga cgcgccaggc ggatgcaacg ggttcggcga tgcttgccga gccgcggtgg
       61 ttctatgacg gccccacgaa gacgttcgtc gtctacctca tgaacataac cgccgacgaa
      121 cggatggcaa aatcggggat ggcaacggtg aggatgagcc tcgcgagcac gaactcacag
      181 caatacgatc ctgtcgggga tatcacagtg caatatgaca aagacgctca caacgacctt
      241 tccgtggcgt gggagaacta cctgaccgcg ctcgggatgc tcaaaaccga cccccacaag
      301 tatgagtggg atatcaacaa acccgtcgaa aaactcgtca taaaagagta caagatcagg
      361 atcctcggca tctaaccccg ctggaacccg tcgccggcag gagggggacg ccctcctgcc
      421 ggccgtgcag aaaattcctg ctcaaaaagg attcagagat accggttctt ccggagccat
      481 tccacctgag gtctcgtgta gcggtagatg atgtcgcact tctcatcctg gaaactccag
      541 ggaacgacga tcaggacatc tccttcccgt atccagacct tcttcttgat cttgcccttg
      601 atccgcccgg tgcgcgtcac cccatcgaaa cagcggattc taatatggtt tgccccgagc
      661 atcagttcag cgctgccgaa catctcccgg ttcctcttgt tcggcaggcg cacgcggatg
      721 atctcccctt ccgcgtctcc tccattggat tcaggtttgc gcttgctagg atcggtcagt
      781 tcatcaaccc catgttccgg agcatctggt ccttcgtgtg tcctatttat acaactgcta
      841 acttatcaag cattctcccg ggacacgcgg aggcggctgc aacggtcggc gatccccccc
      901 tggtcggcca tgtcgtcccc taaatccggt cccgggataa ccgccctctt cccggagacc
      961 accgcccggg tggttatgta tattgccggg atcatcccct attcaatgta cccatgacat
     1021 gcctggactc gtcgcgcggg gtgtacctca cctacttcat ccaggggctc atcctcctcg
     1081 ccgcagccgc aagcatcatg ggaggcgagt acctctccgg gctctcggca gctttcgcct
     1141 tcctcctgac gctggcgccg tccttcgcga cgagaaatat gcgtttctgc ctgccctggg
     1201 gggtcaacct cctcatcgcc gtctccctct atctccacgt tatggggtat accgggggct
     1261 actatgccgc cctcgcttac tatgacaaac tggctcacct cgtctcatcg gccaccgtcg
     1321 cgctcatcgt cttctctctc atggtccttg ccgggcaccg cggagacgtc cgcctgacga
     1381 cgccggcggt cgtcgcctcc atcctgattg ccacgctggc agcaggagcg ctgtgggaga
     1441 tctacgagtt tgcagtcgac cagatattcg gcacgagcct ccagcttggg aacaccgata
     1501 ccatgtggga cctcatcatg gatctcatcg gggcggcgat cgtggcgggg ttcgccgctt
     1561 tcgttcttgc gagggaagac cgatttaccg ggatcttcgc cggactgatc tcggttgcag
     1621 agccggccga tgtcatggat aaccaggcgg attcggcgaa aagccgctga tccgctctcg
     1681 cccggatcgt cggactctat taatatttgg aaggtgaaat aaaatagagt gaccttaaaa
     1741 accccaagtg ccagacatct ggcgttgttc atgctcatca tgctcccgtc catgaccggt
     1801 tacatggcct gggaagcgca gatcgtcgat attaccaccc ttgagatcga cggggcaccg
     1861 gagggtgtcg tctatatcac cgacccccac gtcgggccgt ccagcatcga ccacatccag
     1921 aaggtgatcc aggaggtcaa ccgcttgaac ccctcgctcg tcctgatcgg cggtgacttt
     1981 gccaccggcg acgaggagaa cttcacctac cagggggtat ggggatcgct tgatgcaccg
     2041 gcctacgccg ttctcgggaa ccacgactac cgggtgggga ccgacggtgc gacgggcctt
     2101 gagaggacaa ttgccgtctg gacctccacc aacaccacca ccgccgggta cgacgtctcc
     2161 gccctgaacg acggatctgc cgacaccgca tttgccgacg ggctcgccgc gacgctggag
     2221 gagaacgggg tatacgtcct ccggaacgac tacgcccggg tcgatgccgg gggagaagag
     2281 atcgtcatcg tcggcgtcga cgacgggtgg gccgggatgg cagacccgcc cgacgtgccg
     2341 gcgaccgacg cattcacgat ctatctcatc cacgaaccgt cctgcagggc cgactgggat
     2401 gcagacctca tactcgccgg ccacacccac ggcggccagt tcctcttccc ggtcgtcgca
     2461 cagttaaacg acatcggcgt catcgagctc gccggcctct ttgagacgaa cgggacgccg
     2521 acctacatat cccgtggggt ctcgagcgca ggcatcgccg gcgtgggcct ccggttcaac
     2581 tgccggcccg agatcgtcct gatcaacccg acggaagacc agctccgggt cctcggcaac
     2641 cccgcctgag atcccctcac cgttcgagga tcgcgcgcgc ccggccggca atcaccagca
     2701 cttccgggag ggagagggcc gtgtcccggg cgatggttgt cgcgtcgccg taagcgacct
     2761 gttttgccgt gagtatcccg gcggacgcga gcctggcgga gaccgatgaa ctcatcccgc
     2821 tgacgatggt gacggggtaa gactgcgtct cctcgatcat cgtcttcagg ttctgcccct
     2881 ccggataatc ccacccgata tgctcgatcc cccgacaggc cgcgtagcgc tttgcgtgct
     2941 ccgagaacct ggtgttacag acgatcaggg cgccatcgat cgagacggtg cactgcccgg
     3001 accggaaccc ctcctggagg tcctcgacga tcgcccgggc gatccgtccc tcgtcgaggc
     3061 cggtcatccg gtggtggttt gcgtgatgct tcacctcgac gaatatggtt acaccgtctt
     3121 tactggcgat agcgtccacc tcgtgctctc cgcaccgtcc ggcaagaacg cacccggtct
     3181 ggacctggta accgtgctcc cggaggagaa cccgcacgaa ctcctcgaag tcgggttttg
     3241 ggcggaggag agtgagggct ctccggagat ccgcccggtg gcggacggcg gggcggacgg
     3301 cccgggcccg ggcgcggatc atccggaata tcgcggccgt cttcataccg tccggcaccg
     3361 actcctcgat ctcgtcggcg atccggtcgg catcctccgc cccgagcccg aggttctgga
     3421 gcgtccgccg caccttcccc cggtcgaatg gctgctggtt gccgtcggac ttcgtgacgt
     3481 gcttcattac tgatccatct ctgcacagga gaggaaaagc gttcgccacc atatctttca
     3541 ggcacggccg cacccttatc ttctctcgca tccaacagag gctgagagaa tggatgcatt
     3601 acaacgcccg gtcgtcctga tactcggcgc aggagccgtc ggcctctcgc ttgccgggag
     3661 gctcctttcg gtcgcaacgg tctacgcggc atgccggccg gcgcatgccg gtgcgattcg
     3721 gaagcggggc ctcgtgatgg agggtatctg ggggaacacc acccatggag ggctcgcctg
     3781 tgtcacgggg ccggatgaag tcccgcccgc ggttgacttt gtcatcatca ccgcgaaggg
     3841 caccgacacc cgggcaatct gcgaagagta tgccgggcag ctccgggatc gacccgtggc
     3901 aagcctccag aacggtatcg ggaacgagga gatcatcgca cgatacgccg gcaccgtcat
     3961 cggtgggacc gtgacgacga acttctcggt cgtgggctca gggcacgtcc gggtcgcgag
     4021 cgagagcgcc ccgatgcgcc tcggggtctg gtccggcgat gagagggccc tcgatgagct
     4081 gatcggcatc atccggtccg ccggcatacc ggtcgaggcg gacggcgaca tccgttcggg
     4141 aaaatgggcg aaggccctcc tcaacatcgc ggtgaacccg atctgcgccc ttcttgcggc
     4201 cccggtcggc gccgccgccg atgaagacgt cagggagacc gttgccgggc tcatccgcga
     4261 gacattcgcc gtcgccggcg ccgagggggt gcacctcccc tgggcgagcg ccggcgacta
     4321 tctcgcccac ctctttaccg tgcaggtgcc cgacttcgcc gcggtctacc cctctatgta
     4381 ctacgacctc cagagggggc gccggacgga gatcgatctc ctcaacgggt atgtcgtgcg
     4441 catcggagag cgccatggca tcgagacgcc gtacaaccgg tgcatcgccg gtcttgtccg
     4501 ctacgcggag gcgcacccgg aaccgtcgtg agagattagg cctcgcctat ctttgcggcg
     4561 agtttccggg ccgcctcttt tgcctttgcc tcgctcctcc agagccccgt cttgccgacg
     4621 gggtttcctc cccggtcata gagctgcacg tagtagccgc cgtcctggcg ggcaaaccgg
     4681 acctcgccga cgatgccggg accgtactcc tgcatacccg gagggtatgg aagacggaga
     4741 tatgagggtt tcgcccgccc actcacagtg tcagcgatat gccgaggagg tgcagcacca
     4801 ctgttaccag tacgccaaag atcccgccga ccgcacagat gaggacggtg atcaggttga
     4861 tggggatgtc gggccggccg acgaggtgca tcaggccgag gaggttgata agccagagca
     4921 ggataacgcc cacgacggcg ttgatgatga gcccggtgac gcttttgacc gtcctgtaga
     4981 ggacgaacgc gacgacgacc accaggacca gagcaaggaa tgcgatgggc aagctcatac
     5041 tacctgttcg gcacccgggg cattgaactt tccccggggc accgttcaca tatacccgag
     5101 agcctcaaag actggttgga ggcagacccc gttcccgagt ttgacgcacc ggataccggg
     5161 aatcccttcg taagcgccca tcgtttggcc atcatcaagc tcgacccagt cgtggacggc
     5221 gatgcctcgc ccgaagacct ccatgtggag cgcatcggtc cagaacgtct ccgcatccca
     5281 gaccatgatc ccgtggaccg gccgggcggg gatcccgttc cggcggagga tctccaccct
     5341 gaggagtgca aggtcggtgc agtcgcccgc tccacgggtt agggtcgcgt tgaggcccac
     5401 cgggaagagg cgggggacac cgtcgacgcg tgcaaacgtc gcccgatcca cctgctcgat
     5461 gaacccggtg caggaagaat ccgtactctc ggaagagagg atctcttccg gtatagagaa
     5521 ccaggcccca tcgtcgatac agaacggttc ggggccaccc catccgccgt cgcagatcag
     5581 gtgtccctgc gggatgaggg cggagaggag gagaatagcc tgaataatgc tccaaaccac
     5641 gataattgct caatactttt taatgaataa attagtataa tagaattgct atttgatgtc
     5701 gagtattgcg ggttcgcaat ataactatcg cgaccgtaga tacgccggga tcggctctgg
     5761 cgcgcaacaa ggaatatatg cccccgggcg ggagggagaa gaggtcgact ccccggaggg
     5821 cgacaggttc accgggatat ggggtgcccg gcgatatccc gtgcggcaag ggccgcggtg
     5881 gagaatgccg cctgcaggtt gtagcccccg gtgtcgccgt cgatatcgag cacctcgccg
     5941 atgcagtaga ggttccgcac gacccgcgag accatcgtct ttgggtcaat cccgtcgagg
     6001 gcgatgccgc cccgcgtcgc catcgcctcg tgaaaccccc cgaggcgtgc cacggtgaat
     6061 ggaaactccg cgacggatgc ggcaagcgca tcccgggcct tccgggggag gtgggcgcat
     6121 gtcgctcccg ccggaacccc ggcgatatcg agcagccgcc gggcgaaccg ctccgggagg
     6181 gggaggtcgc agaggatggt ctttgcctgc cgggccccgc cctcccggag cgcgccggcg
     6241 accctttttc gcaccgcttc cgcgtttgcg ccgcagagga acgtgacccg gagcacgtcg
     6301 cccgggagga tgaaacggga agcgtcaagt atgcccgggc cggagaggcc ggcgtgggtc
     6361 agcaggatgt cgcccgtgtg ccgccggacc acccttccgc cccggtgtat ggcaaaggcg
     6421 gcattctcaa acgatatccc ggcaaggtcg gcgaacgggt agccctcgat ggagaccggg
     6481 gtgagcgccg gggcaacctc cgtgacgggt tgcccgaggg cccgggcaaa ggcgtaaccg
     6541 tccccggtag aaccggtggc gggataggag gcgcccccgg tggctatgac gaggacgccg
     6601 gcccggacac tctccttctc cgtccggacg aggaacccgt ctccttcccg ctccacggcg
     6661 acgaccggct cgccgcaccg gataacaacc ccgcgagcgg agcactccga aaggaggacg
     6721 gcaagcacat cggcggctcg ccgggtctcc gggaagacct ttcccccggg ttcggcctgc
     6781 ataggaaggc ccctgtcggc aaagaaggcg atgagatccc ggttcgtgaa gtttcggagc
     6841 gcggggcgga ggaacgcccc atgatcgccg taatgggaga gaaaaccggt gacctccccg
     6901 tcatgggtga tgttgcactg tcctgagccg gtgatcagga gttttcgcgc cggtgcaggc
     6961 ttcttctcaa ggaggatcac cctcccccct ctccccgctg cctgcagggc gcagaagagc
     7021 cccgcaggcc ctcccccgat gatcgcgatg gtgtcgctca cgattataac cctgtaccgt
     7081 gcttccccgg ggaacatcag cgttggggtg cgccggccga tacacatatg ggagtggggc
     7141 ccgtaatggg ccggagccct gaaacgagtg atccgaatga atgatacctg gatacttctc
     7201 atgggaggag tcctgctcgt cctcctcatc acacccggat ctgctgcctc tgaaggtgca
     7261 cccggcaccg gaggttttgc cggccggcag gcgatcgcga tcgaccaccc ctccgggacg
     7321 gaagggcttg aggtcctctt caacgccacg tacctccccg ggatgcggcc ggacttcgcc
     7381 gacatacggt tcctggacgg gaacgagacc gccatccccc actggacggc aaccgtcatg
     7441 gagggctccc atgcccacgt ctggcttgcc cttcccgcga acgcgaccac gatcaccatg
     7501 ctctacggca acccggcggc ccgggatgca ggcgaccccg acgccgtctt cctcttctac
     7561 gaggactacg agcgcggcga cctctcccga tggtcgttct gcggctcaaa cgctgccgtc
     7621 cggtcggatg tcgtccggag cggtggatac gcaggcgata tcaacgtctc cgagcccgac
     7681 ctccgggaac tcgcaaacaa ccggatctgc cttgcaaata tatccgccgg ccccatcatc
     7741 gtcgaagggg acttcctggt cagcaagttc gggaaatggt gcggatcggg ctacatccag
     7801 gcctggagcg ggcccgaccg gctctatgcc ctccatctca ggaacggctc cgtccagtac
     7861 tacgacggcg cccaccggaa cttctccgcc gatgcccggg cggaggagga gacctggtac
     7921 cacatcaagg tcgccctcga tacgccgaac gccaccgggc acgcgtggat ggacggccgg
     7981 tacctgggga agatgaccct gcggttcgcc gacggttcac ccgtcccgga ggagacgatc
     8041 tttaccgaca tcgccctgat cgggtcttcc gcatgggggt caggcgaccc gcaccacttc
     8101 tgtgccgaca acgtcgtcgt caggaactac gtccccgtcg agccggtgct ctcctacgag
     8161 gtcaatgctc cgggatgcac cggggaccca tagggcgttt tgtcaccgcc ctccgtgcgg
     8221 ttcgccctgc tgtgccggtt gtgcggatcc cctatcgagg ggccgaccat ccccgccggg
     8281 gatccgttct accgggcagt agagatgcgg tcgggtcggg gatgatccgg gatcacccgg
     8341 tagactgcag gacgtggagg agccgccgga cggcagtggc gttcgtggca actgaaagga
     8401 tgacaagcgc cgggaggacg agcccggtga ccagcccggc gatgatgacc gcaagtcgtt
     8461 ccggccgggc gaagaggccg gcccggcact ctatcccgag cccctcggct ctggcccggg
     8521 cgtaactgac catcaacgac ccgatagcgg ccaagaacgc agcaacggcg ccggagggtg
     8581 atccagaccc ggcgtagtgg acgagcaggc cggcatagac cgccgcctcg gcgtagcggt
     8641 ccaggaacga gtcgagaaaa gcaccgaaga ccgaggcggt accgcttgcc cgggcaagcg
     8701 ctccatcgag cgcatcgaat atacacgacg ccgcgacaac gacgccggca aagaagaacg
     8761 agccggcggc gcagagggca ccggcgaccg ccatcccgac aaacccgagc acggtgaggg
     8821 tgttcggggt cacgccggtg cgggcgacga gagtggctgc ccccacgagt atcccgctcg
     8881 tccttgcccg gagccgctcc tcgatcaccg gcccgccccc cgggaaggaa ggaccccatc
     8941 catggcaggg aatcgtctcc ccgggagata agggtgcctc tcccgggagc ggacgttacg
     9001 cccgcaatta tcatggagct ctatggccgg ggcctttccc cggcagtccg cccggttccc
     9061 ggagtcgttc acaggcccgt atcgtgtcta aatcgcgcgg aacttctcgc ccttcttgcg
     9121 caccagtccg accttcttga ggtcgttgag gagccctttc acgaattcgg atgtgagctc
     9181 cagcgatatg ctataggcct cttcggtgcc ctcaagagga gtgccgttga gcgtctccag
     9241 ggcctcctcg ggcgtcagcg ttccgcgttc ttcgaggatc tccagggttc ttgcgatgac
     9301 ggctctcttc atggaaacgc ccgcccggaa cgatatgtac tcctcttcat cgatcccgag
     9361 gtcgtcgatg atctcgtcaa tcgtatcgag atccccatcg atgatggcct caggaccgaa
     9421 gatcagcagg tgttcgggca ccggcgtgac agtcaccgcc atcgtgacgt tgtgctcttt
     9481 gagagtctcc ggaataatct cctcggcggc ctcgcccggg atggtgatga tgacatcctc
     9541 aggatccata tgccccttca cggtcaccgt ctcacccaca atctcgatca ggccgttttt
     9601 atccagcatg gagagcgccg tcatcttgta taatatggac ttcgccaggg catccgagcg
     9661 ttcctctccc ggggcaacat cggcgagtgg cgccatccac tctttgaggt cggccataga
     9721 gataacatcc ccgggccggt acttctccat gaaccgggcg atatcctccg cgatgcactc
     9781 cgtcatatca aggtaggggt catacagatg cccggtatcg aaatattcgt cctcccagtc
     9841 atcggcatct tcctcgtctt cccggtcatc ggcgccctcc ttgtcctccc ggtcatcggc
     9901 gccctccttg tcctccgggg cgtcgaactc ttctccctct tcatcagcca gaaattcctt
     9961 gcggagtttc tcgattcgcg ctatctctcc cttcagcagc gccctgatat ccccgatcgt
    10021 tcccttcgca gcaacccggc cgtccagggt aaccgtctgc gtgatacggg tcggcatacc
    10081 ggcctccctg aaggcgcgcg cgatctccgg gccgtcgata gggccacgga tgatgatcat
    10141 ctcttcgagt accatacgtc ttccccacac tattgacgta cctgctcata aggtcgccga
    10201 taaaggagcg cgagaaagtc cctttttgat gagatactcg tgtgcttcaa gcgccgcctt
    10261 cgccccttcg cctgcagcta tgatgatctg cttgcccttg atgcaggtcg catcgccggc
    10321 cgcaaagatt ccgtcgacgt ttgtgtgatt gttctcgtcg accaggatct cgtgctgctc
    10381 gttctgcgcc accagatccc cgaggaaccc cgtattcggt atacgcccga tctcaaggaa
    10441 gagcccctcc acgtcgagag ccgtctccct cccggtctcg cgatcgcgaa ccgtgacgcc
    10501 agccagggct gtatcgccgt ggagctccac cacctcgtga ttggggaaga cccgcacacc
    10561 tgcctcctct gcccgcctga tatagatctc gtcgcaccgg agagcgctcc tgacaatcaa
    10621 cgccacggaa cttgcaattt ctgtcatctc gacagctgtc tggatcgctg cattcccccc
    10681 gccgacgatg gcgataggcc gatcgcggaa gagcggtccg tcacaggtcg agcagacgga
    10741 tatacctctt ccgatcaacc ggtcctcacc cgggagcccc agggtccggg gctgtttccc
    10801 gggggcaagg atgaccgtcc ggcaacgata ggtgctggac gcccggacaa aaaagaggtc
    10861 gccttccttc tggatacccc ggacccggtc aagctcgaga ccgacatcga acccgcggac
    10921 ctggtcctca aacttcccga taaggtcctc ccccgtgatc atccgaaatc ccatatagtt
    10981 ctcgattgcc cagctccagg ctgcctgacc accgatatcc tccgacacga cgatggtctt
    11041 catgagtttt ctcgcacagt agaccgcggc cgtcaaccct gccggccccg cacccacgat
    11101 gaccgcatca tagatctcat ccgccaccgg cgtcccgaag agttcccgga gcttcttcgc
    11161 atcgaacccg acgatcacct catcgccgaa gaccgtgacc ggcacgcccc gctgtcccga
    11221 tatccggacc atctcccgcg ccgcctcgcg ttccttcccg acatcgacga tcgtgtattc
    11281 gacaccgtgc ttctggagaa acgcctggac catccggcag tagggacagc tctccgtcgt
    11341 gtagaccttc acgcctgcca taaggaaagg atagttcggg agctctattt aacttgtggg
    11401 gatggagcgc gcgggagatc cggccggagc gatacggggc aaaaaagagg gttattggac
    11461 ctcgcggaac atccgcgccg ggaccccgca gatggggcac ttctccggcg ccgctccgag
    11521 ctcgacgttg ccgcagaccg ggcagagata gatcttctcc acgtcgatat cttttccctc
    11581 ccggacagcc tcaagtgcct ggaggtagag gtttgcgtgc acctcttcgg ccttcatggc
    11641 gtgggtgaag acgatcgcag cctcattcct ccgctctttc ttcgcctctt cagcaaacgt
    11701 agggtacatc gtcatgaact cgtagttctc cccctcgatc gcgcctttca ggttctcttc
    11761 ggtgctcccg acggcgttct tgatgaagag gagacgtttt gcgtggacgg cttctgcctc
    11821 gcttgccgcc cggaagagtt tcccgaccac cgggtaacct tcggcggttg ctttctcggc
    11881 aaagaccgag tatttcctgt tcgcctggga ttccccggcg aacgcttcct gagcgttctc
    11941 atctgtcggc ataacgaatt atttcggagg tgccccgtta aatatctttc acccggcgcc
    12001 cccggaaacc ctatgccgcg ggagcgacta ttaccggtat atgcagggtg accgggtgca
    12061 acgggtcttc gtcatcgtcc tcatcttgaa tgtcgccgtt gcagtagtaa aagcggtctt
    12121 cggcgtcatg gcaggatcgg tgagcatggt ggcggatgct ctccactcgg gattcgactc
    12181 cttctcaaac gtcgtcggga tcgttgccat gcaccttgca gggaaacccc ccgacccgga
    12241 gcacccgtac ggccacggca agatcgagac cctcgggacc ctcgtcatag gggcgatgct
    12301 cctcctgacc gctgccggca tcctccttga ggggtacggg cgcctcatta cccccgtcac
    12361 tcccgagatc acccccatca ccgtcggggt catggtcggg acgctcatca ccaacatcat
    12421 cgtctccacc tacgagcggc ggaagggcga ggagtaccag agccagatcc tcatcgccga
    12481 ctcgttgcac acaaggagcg acgtcttcgt ctccaccgcc gtcctcggcg ggttcctcgc
    12541 catcaggctc ggctaccccg cggccgaccc gatcatcgcg ctcggcatcg gcctcctcat
    12601 cgcgcgaatg ggtgtcggaa tcctctacgg agccgcggaa gtcctcatcg actcgatgaa
    12661 ccttccctgc gacccggcgc tcgtccgggc cgtggtgatg gatacccccg gtgtcgccgg
    12721 ttaccacgac ttccggtgcc ggggtaaacc gggcgagata tttgccgaca tccacgtcac
    12781 cgtcgacccg gccctctcca tcgcccgggc ccacgcgatc tccgaggagg tggaacgaag
    12841 gctcaaagag acggtgccgg gactcgccga ggtcgtcgtc cacatcgagc cggacgattc
    12901 agcgtgagga ggagaggagt tgcccgagct cgtaaccctt ccggtaggcc gcctccatca
    12961 cctccggccg cgtcctgata tcctggatgg tgtccatatc gtttcggagc acctcatccc
    13021 agtaggggca atcgacgatc tctccaaacg ccttcgcggt catcttcgcg ccctcaaaga
    13081 cggtcggaag gttcatcccc gctatcgata tgaagagcat cttacgcctc ctcctctgtt
    13141 caggggatat aaagggcctg ccccggtgat acttcgccat gtaaaagacc tggaaccggt
    13201 ccatgaacga cttgagcctg cccgggatcc ccatcgtcat aacgggagtg gcgatgatca
    13261 ggccgtccat ctcccgaaat ttgtcgtata tcccgatcat ctcgtccctg atctcgcagg
    13321 tctcattttc ctggcagtaa aagatctcca tacagggcgc gatatccagg tgtgcggcaa
    13381 cgatccgctc gacgtcgcac ccggcctcct ccgccccccg gatggcctca tcgaggaggc
    13441 gagcggtgtt cccgtcgggg agcgggctcc cgagaagagc aataaccttc cttcccatga
    13501 tgctgggtta cacacggccc tattaataag atgcggctct ccggggcccg ggagcccgct
    13561 ccggccttgc ccgtgaggat actgctaaat actttgaaac gatatgatcg ctctggtgag
    13621 tgcctgtggc cgaaccagga accagaacga cagcaaaagc aggagagaag ccgggccccg
    13681 gacggttcat ctgcatcgac tgcgggaaag aagtccggat cggcagcacc gacgaggatc
    13741 tcgtcaagtg cccgacctgt gcctgtgaga tgtacaactg tcttccgatg acccacatca
    13801 ggccggatat caagacgccg gaggatgtca tgaatcctcc tgaaaggaag aagtgaccgg
    13861 aaaactatca aggtgagaga tgtggtgaac gtatcagcaa aaggtgagat ttaccgctgc
    13921 gagatctgcg gaaacgtggt cgaggtcctg gaggccggcg gcggcgagct gatctgctgc
    13981 ggcgagccga tggtgcttga ggagtgacgg acgtggcgga agagcagaaa aacctcgaag
    14041 agaagattgg aaagatcccc gagatcttca aagagctcaa agagacggat cccgaccttt
    14101 acgggaaggt gatggggctc gatcagatga tctgggccga cggcgccctc tcaaagcaga
    14161 cgaagaaggt catcgcgatc gcgatcgccg ccgctctccg ggatgggcat gccgtccgcg
    14221 cacagatggc cggcgccggg aatctcggcg tatcgaagga agagatcgag gaaggactca
    14281 gggtcgcgtt cctgcttgca gggatgccgg cctacgtcca cggcaagacc gccctcgaag
    14341 agtatctggg cagccgcccg cggaagtgat acgatggaac cagaaggatt accccagatg
    14401 ccggttatcg gcgagaaagc acccgaattc gacgcattga ccacccatgg acctctgaaa
    14461 ctctcggacc tcgcaggaaa gtgggtcatc ctcttctccc acccggccga cttcacgccg
    14521 gtctgcacca cggagtttat cgcatttgcc gggatggctg acgagcttgc aaaactgaat
    14581 gtccagctcg tcggcctctc gatcgacagc gttcactcgc accttgcgtg ggtcaggaac
    14641 atcaaggaga agatgggcgt cgatataccg ttccccatta ttgccgacct cgacatgaag
    14701 gttgcaaagc gttacgggat gctccacccc gggcagagca gcacatcgac catccggacg
    14761 gtcttcttca tcgacgataa gggcatcatg cgtgccatgc tctactaccc gatgtccagc
    14821 ggccgctcca tgcccgagat cctcaggctc acgaaggccc tccagaccgc cgataagcac
    14881 catatcgcga cgccggcgaa ctgggaaccc ggtgacaagg tcatcgtccc ggccccaaag
    14941 acacccaaag agatcgaaaa gcggatgcag gaaggctacg agtgcaaaga ctggtacctc
    15001 tgcttcaaaa aactttaaat ccgacacttt tttgccttca gccggatagt actcactgta
    15061 tgaccattga tgtcctcgcg tttgcgacat cgccccgccg ccgtggcaac tccgagaccc
    15121 tgctcgactg gatgctcgat gcgatgaaag aggaaggggc agcggtcgag aagatcgtcg
    15181 tcccggaagt cgatatccgg ccctgccggg ggtgcaacgc ctgcgcgaag ctcaaccggt
    15241 gcatccagcg ggactacatg gactacgtcc acgaccggat cgtcgaggcc gactgcatca
    15301 tcctcgcgtc ccccatctac tgcatggggc ttacggcgca ggcgaaggtg ctggtcgacc
    15361 gtgcgcaggt tttttgcacc cggaagtacg tgcttcgcct cccggtcgtt cccccggagc
    15421 ggaagggaaa gcgtgtcggg gtcttcctct cgaccgccgg gcagaactgg gaccatgtct
    15481 ttgatgcggc gataccgtcg gtgaagtgct tcttcaacgt catcgacgtc agggataagg
    15541 atatccggta cctgatggtg aacaacgtcg acgagaaagg agcgatcgcc tgccatccca
    15601 ccgcgagagc agacgccgcg gccctggccg gagaggtcgc cgcgtacctg caggaggtcg
    15661 gtgtggcatg accgaccccg tgacggtcct cgggatatcc gggagccccc ggcggcacgg
    15721 aaacaccgag accctgcttg atgccgtcct cgaggggacc cgggaggccg gggccgacgt
    15781 ggagaagatc gtcctccggt cgcttgccta caccccgtgc agggggtgca acgtctgcca
    15841 caagaccggg gtctgtatca tcgaggacga cctcacggag attttcgata agatcgccgc
    15901 cgccgacgtc ctcatcatcg cgtctcccat ctactcgatg gggatcacgg cggagacgaa
    15961 ggggttgatc gaccgtgccc agtacctctg ggcacgcaag ttcatactga agaccctcta
    16021 cttcaccacc gaacagaccc gccgtcataa ggggatcttc gtatcgactg cggggctcgg
    16081 gtgggggaac gtcttcgatg cggcgtttcc cgccatcacc gccttcttca acaccaccgg
    16141 gtttgagtac tgggacaacg tcatagcaaa cgatctcgac cgttacggcg ggattgcggg
    16201 ccaccccacc gcactcgcgg caggccggga gaaggggcgg gaggtggtcg ctctcatccg
    16261 gaagatgcag gcgcgagaag gcacggagac ctgatcccct ccgggtcggg tgggcatcaa
    16321 tactatttcc ccggctgccg gatcccgata tattccagca gggtcccgat gtgaagcgcg
    16381 ggtaatattt tcccactcat tcgggatcga agtgtattaa tacggctatg gtaagataac
    16441 acccctgtaa ctacccccga gatgcttttc agggccacag aaggatacgc aggtgactcc
    16501 tatcaaaccc cgggaaaccc ctgatgaggt gccactggaa ttacggttac ttgacggcat
    16561 tgatgactgg cttctcctga tcggcaggga tcacaacgta atccgggtaa actcggccat
    16621 ccgccgtatc cacggggcac tggagggcga cctcgatcag gctgtctgcg cactcggcgg
    16681 gagcagaggt cttgccggga ggatccgcac cgcgctcgac gatgaagatg agatccccgg
    16741 gtttgagtgc cggctccagg gcccgggcgg aggggagcgg cggtatctct gctcggggtg
    16801 gaggatgccg cccggggagg gctggttact ccggttccgg gagatccccg ttgccgggga
    16861 ttacgaggcc gtcgtcgagc ataccgggac ggcgacgatc ctcatcgagg acgacggcac
    16921 catatcgctt gcgaacacgg agttcgagcg gctctccggg tactcgcgcc gggagatcgt
    16981 cggggtgaaa cggctgaccg atttcattgc ctccgaggac gagtgccggc agatcagaga
    17041 ataccatatt ctccggagga gtaaccccgc ggcggcgccg aagaactact cattctcgtt
    17101 catcgaccgg gccgaaaacg tccatatcgt cgaggccacc atcggcatga tgcccgggac
    17161 ggcccgggcg gttatgtcgc tcatcaacgt gaccgggcgg aggcgtgcgg agcaggcgct
    17221 gcaggagagc gaagagcggt tgaaccttgc attcctggcg gcaaacgacg gactgatcga
    17281 ttgggacgtc cgcaccggca aaatctttta cagcccgcgc agttttacga tgctcggcta
    17341 cgaaccggga gcgttcgtgc ccacgtaccc catgatcctt accctcgtcc atcctgagga
    17401 ccgcgatcgt gttggggagg cgctcgccga tcttacctcc ggccggcgcg accgctgcga
    17461 tatggagttc cgggtgcggt cagcatccgg ggactttgtc tacgtgctcg accgactcca
    17521 ggtggtcagc cacggtgccg gcggagagcc gctccggatc gtggggacgc attccgatat
    17581 cacggagagg aaagaggcgg agagagaact ccggatcaag gatatggcga tagcgtcatc
    17641 gctcgatgcc atctccctca ccgatcttga agagaggatc acctacgcca accgtgcttt
    17701 cctcaccatg catgggtggg atcgggagga gatcatcggg cagccggctt ccgtgctctg
    17761 ggccgatccg gaggaggcaa aaagggtcat cgcggagttc cttcgggacg gcacctggtc
    17821 cggggaggtg gcggggcgac ggcgggatgg gtcgaccgtc catatgcaac tcgccctctc
    17881 cactatcacc gacgaggcag gagcacggct cggttcaatg ggttccggga tcgatatcac
    17941 ccggcgaaaa caggctgaac gggaacttct gattcgcaat atggcgatag cgtcctcgct
    18001 cactgcattc gcgatcacgg atctcgacgg ccgcctgacc tacgccaacc aggcgttctt
    18061 ctccctgtgg ggctacacct ccgctgctga ggtcgtcggg aggcgggtta cggggttctg
    18121 gcaggatgaa ggggaggcgg aggatgccgt ccggatgctc cttgagaccg gcaggtatac
    18181 cggcgaacgg gtcgggagga ggcgggacgg ggcgaaattt tatgccatgt tctccggaaa
    18241 cctggtcagg gatgaggccg gagcacccat ctgccttgcg gcctccctca ccgatatcac
    18301 cgatctgaaa cgagccgagg cggagatcca ggcccataac cgggagctct cggtcttgaa
    18361 ccagatcatc ggggtatcca catccgcgac cgggatcgat gaggcactcg gggatatgct
    18421 cgcggcgacc atcgccctcc tcgatctccg tggcggcggc gtctacctgg tcgaggcgga
    18481 ccggcagaga gcacggctta tatgcacccg gggtctcccg gatggttttc ccctgcagac
    18541 gtgcatcccc gacatcaccg cacccccgta tgcggatgtg ctggtggcgg gagagccctt
    18601 cttctttgac gatcccacgc acctccgcta cccgggggag gagagggaga tgttgcaccc
    18661 ttacgccagt atcccgatcg tagcatccag agggatcatc gggtcgttga acgtggtgcc
    18721 tcgcgaccgc aggcggttca ccgacgccga ccgctccctc cttctcgcca tcggaaaaga
    18781 ggttgggagt tctcttgagt gcatactgct gatccggcag cttgaaagcg ccgagcggga
    18841 ggcgaacctc tacctcgata tcctcagcca cgatatcagg aacgcggaga acatatcaag
    18901 tctctacatc gatatcctca tcgatatgct cgaaggcgat gcgaaggatc acgcacagaa
    18961 gatccggagc agtatccgaa agagcatcga gatcttacgg aacgtctcga cgatccgccg
    19021 gatccatcac gagtcggtca cgctcgcgcc ggtcgatctc gatgaggtga tccgggacga
    19081 gatcggggta ttctccgaga tcaacttcaa ctatgaggga accgatcacg cggtcatggc
    19141 cgatcccctc ctccccgagg tctttacgaa cctcatcggg aatgccgtca agttcggggg
    19201 gtcggcggtc gagatcacca tccgggtgga ggagagcggt aacgacgtcc tgatctcggt
    19261 cgaggatacc ggaccgggga tctccgacga gatgaaagat accgtattca tgcggtttgg
    19321 aaagaacagg atccagaaga gcggccaggg cctcggcctc tccatcgtcc ggatgcttgt
    19381 cgaacgctac ggtggcacta tccggtccga cgaccgggtt tcagggtgcc ccggatgcgg
    19441 cgcggcgttc cggttcactc tgaagagagc ctgaaaattg ggtggggcct cacaggagcg
    19501 accagaagag cgccccggcg gacgcgacca tgagcaccgc aaacacggcc gggttccagg
    19561 cgaggatctt gcgcccatcg aggacgctga tcgggagcat gttgaacgtc gcgatcatgg
    19621 cgttgacccg gaaaccgacg aggcccacga taccgatgag accgccgccg aagagcatga
    19681 ggagcgcaaa cggtatgcag aggaggaggt tcgtgagtgg tcctgctgca gatatccgcc
    19741 cgttctcggc ccgtgtcgcg ttcccgtaaa tatacgtcgc tcccggcgcc gcaaagacga
    19801 ccccgacgag cgccgccatc accacggcga cgaggagcat ctggttatcc ttcttgaact
    19861 ccgcccagta gccgtagcgc atcgcagtga acttgtgcgc cagctcgtgg agaatgaagg
    19921 cgacccctac cgtgaggagg gacatcacga agaagaagat gagcccggtg acgttcaccc
    19981 ctccccggat gtagatcagg gtgaaggcga ttgagatggc gagccacgcg atcaggagat
    20041 cccggcgctc gcgcagcggt attcgttcca gcatttggta cagggtttgt cccggcacga
    20101 taatagcctt cgcctcttcc gggagatagg cacggacctg gaaggttatt tcccggtcgc
    20161 ggccaaccat ggagtatgtc ccttgatgcc gtcaggagcg agatccgtgg gatcgacgag
    20221 aagatcatag atctgattgc agagcggcag agacttgcgg cgaggatcgc ccgcctcaaa
    20281 caggaggagg gcctccccat ccgcgatggg ccgcagcgga aggcggtcct cgaccgggtc
    20341 ttcgctcacg ccgccgagag ccggatcgac ccggtcgccg tacggaatat cttcgagatc
    20401 ctgatcgata tgaacgaaga gcggcagcgg gagtgcagcg gggatgggaa cctgccgtga
    20461 aaaaaaagat tagagggtga tcatcgtccc gacgccctcg tcggtgaaga gttcgaggag
    20521 caggttgtgc tcccggttgc cgttcacgac gtgagcgctc gtaacaccgc tcctgaccgc
    20581 cctcatgcac ccgtcgagct tcgggatcat ccccccgctg atgatccctt cggcgatcat
    20641 ggtatccgcc tcagacagcg taagacggtg gtaagtcatc gtccggtcgg cgttcatcac
    20701 gccgtcgaca tccgttaagt tgatcaactt gaacgcctgc agggcaatcg cgatctcggc
    20761 cgccgccgta tcggcgttga tgttcaggct cccgccctgc cggtcgatcg ctatcggggc
    20821 cacgaccggg atatagttct gggcaaggag gcagtggagc acctcggggt cgacctcctc
    20881 gatctcgccg acccggccaa ggtccacctc ttcctcgacc tcccccaccc tcacccgctg
    20941 gggctccatc ttccgggcga tgaggaggtt gccgtcgttg ccgga
//
LOCUS       DAOSEE010000021        20802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1096_length_20802_cov_247.815250, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000021 DAOSEE010000000
VERSION     DAOSEE010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 20802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20802
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1096_length_20802_cov_247.815250"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PLI76_02900"
     CDS             complement(<1..420)
                     /locus_tag="PLI76_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HOI61009.1"
                     /translation="MQYEITGDNLQMVTLRLASGESACAEAGAMVNMSGNMQMTTHMK
                     GGLFKGLKRMVTGEGLFMTEFTPQGGGGFVSFAGNIPGKIFALNLDGGEFIAQKDAFL
                     CSEKGIDLDIAFTKRLRSGFFGGEGFILQRLSGRGNVF"
     gene            625..930
                     /locus_tag="PLI76_02905"
     CDS             625..930
                     /locus_tag="PLI76_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61010.1"
                     /translation="MRPIVLVLLLIAVAGVAGTEAINVSLPDRDAPEAVLGTVESMWG
                     FVEWLLQVADDLLSLIRNVLGSLGVGGEEVERLMGAMDSGKDLVNRTLEEGAPSGRG"
     gene            1346..1609
                     /locus_tag="PLI76_02910"
     CDS             1346..1609
                     /locus_tag="PLI76_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61011.1"
                     /translation="MGRVTGLQVDDLVGIKDDTDAHAGHGGGAALVGSESRTRTRGVV
                     LVGPAQALGTTLVRALDPRAAVEGSGRPKGHRKIYPPGGHLPA"
     gene            1615..1893
                     /locus_tag="PLI76_02915"
     CDS             1615..1893
                     /locus_tag="PLI76_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOI61012.1"
                     /translation="MRGGEEAAHPAEAITAGAHLTDEARSAALERQCHRCAGCNAPLT
                     AGSTHFDIRPPVICGGSPLPGNLEALCPFCHQNFMRRVRVRLAGHRNK"
     gene            1917..2195
                     /locus_tag="PLI76_02920"
     CDS             1917..2195
                     /locus_tag="PLI76_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61013.1"
                     /translation="MTCRRHAAQSDEWRSTVMRRADEIAGRLALPPSPLPGGWRSGTM
                     QMLDAAVTPGTVQNIAASLAVAMFVIPRIPCRPLAIAAGFAAGSIFGK"
     gene            complement(2199..3998)
                     /locus_tag="PLI76_02925"
     CDS             complement(2199..3998)
                     /locus_tag="PLI76_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOI61014.1"
                     /translation="MSGQPEFLPMTGEEADRLGIDRFDVILISGDAYVDHPSFGTAIV
                     GRVLWNAGYSVGVIAQPDWRSDADLRRLGEPRLFFSISAGNVDSLVNAFTPNLKRRSS
                     DVYSPGGRLLRPDRATLVYTDRVHALFPETPIVLGGIEASLRRFAHYDYWSDSVRQSI
                     LADAPADLLVFGMGERQVVTIADRLAAGERTLTDIPGTACRVDRKTWRGMDPTGYVVL
                     PDYAEVKSDRYAYARAFALHYNEQDPFRGRPAAQPHPKTVVIQNPPAIPLSGPELDRV
                     YELPYTRRAHPSYAEPVPALEPVRFSVVSHRGCFGACSFCALTHHQGRIIQSRSADSI
                     VREVERMAKMPEFTGIVQDIGGPTANMYGIHCGRWETAGTCPDRRCIDCPALDRSHAD
                     QLRLLRRLLDIPGVRRVFIASGIRYDLIGPEDEEYLSRICADHVSGHLKVAPEHVSAR
                     VTACMGKPPRAVFDAFRERFEVLQAGKKKRQYLVPYLISGHPGCRMEDMVELAEYVRD
                     TGLYTEQVQDFTPTPMSISTTIYHTGLDPFTLEEVYVPKGREKQIQRALMHYRDPANY
                     ALVREGLRATGREDLIGSAWKCLVPARGKRRSP"
     gene            complement(3995..4444)
                     /locus_tag="PLI76_02930"
     CDS             complement(3995..4444)
                     /locus_tag="PLI76_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF296 domain-containing protein"
                     /protein_id="HOI61015.1"
                     /translation="MQYSEGQVGRVFTVRVDDGEDLIKEIRRFVAAMNVQSGMVHFLG
                     AVRTATLVTGPKEPVVPPTPRREEIFGGWELLGFATVHPGEDGPSLHIHATAGRGIRS
                     LTGCLRDKAEVYIVVEAIITEFVGITSRRLPDEKTGVDLPVFDGFLP"
     gene            4531..5661
                     /locus_tag="PLI76_02935"
     CDS             4531..5661
                     /locus_tag="PLI76_02935"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOI61016.1"
                     /translation="MVAGIWFREVEYLRGFAALAVIAVHVSMNFTRLPGAGLPALVGI
                     FVYIAAHFAVPVFVFISGWVLAARYRGGYPVPAYYRRRARTILPPYILFSALYLPIVV
                     EGAMAFAGMPAPGAMVGALLMGTAAYHLWFFVVIIQLYILYPLIAGVYDRFDRAGAAL
                     FLLLALLFVQVLWNVGAHAAGAFAGTEWYAVLIRLFPSHLLYFVLGVHVARHTDRFRS
                     MPGSLSPAWVVAAAGAGALLTGGIWVAAVYLSGGFAGATLGVFCVYRIIEPFYYVPVI
                     AALVVAARRLERGGGRPAGAFRSFGAHSFGIYLIHPLVIAAGAAAWAALTGWTWADWP
                     TYPVLFVVAAAASYGAVRAASAVPYAGYLLGEPRALPATERS"
     gene            5868..6086
                     /locus_tag="PLI76_02940"
     CDS             5868..6086
                     /locus_tag="PLI76_02940"
                     /inference="COORDINATES: protein motif:HMM:NF022829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HOI61017.1"
                     /translation="MPEQISTPSYQVYTKGIDYPRSKQEVISYVREHNAPDDVIRVME
                     EMQDQQFTSASDLAVAFGEAKDRLEGRR"
     assembly_gap    6267..6366
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7235..9298)
                     /locus_tag="PLI76_02945"
     CDS             complement(7235..9298)
                     /locus_tag="PLI76_02945"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HOI61018.1"
                     /translation="MILALLIGLLFLAGGVLIALMLYCYKHRDIALARPLGLLLLCAA
                     FLPITHALNLMTETLWLKIFLLQIRFLAVPFLAVFQLIFILTYLRREEWIQGWKKVSL
                     FIIPIMTVILAQTTWFHPLYRYDYWVDLSGPLPILNFTNGIGFQVHVAYSHILLIASL
                     LLLIFVQPEIHAFYRRQRLLLLAALLLPLLCDLQYQLNLTPYPGLTLTPFALMFTGLV
                     FVGATLRQGLLEVAPVARGKVIEEMSSPMLVIDARGRMTDMNTAAVRLLGTVHDDAIG
                     SPADLVLAGHPSVLALAQGASGDNREIAIGEGLERQFFDVAVDDLRTGTGAVIGKLVL
                     LQNITERKQAEEALKESEEKYRTIIENMQDLFYRTDLDGTITMISPSGVALAGYGSPE
                     EMIGVTMQELYADPLERERFLVALRANGAVGGYPLVLKMKDGTLKHVTVNGHFFTDAE
                     GSVRGVEGIIHDVTDLHLAEQALRQANRQLNLMSSITRHDILNQLTVLLGNLSFAQEE
                     VSEGPLSGYLEKMSIAAGTIQRQIEFTRDYQDLGVRAPEWQRVSGAIRTAAAGGLPIT
                     DETDGLAIYADPMLVKVFANLMDNTIRHAESATRVHLRYRPEENGDLTLVWEDDGVGV
                     PAGEKERIFDRGFGKNTGLGLFLIREILAITGITIAERGEPGEGARFEMRVPSRAFRF
                     GSEAV"
     gene            9720..10622
                     /locus_tag="PLI76_02950"
     CDS             9720..10622
                     /locus_tag="PLI76_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HOI61019.1"
                     /translation="MSTLESYLDALHRGVFQVVVPKALVTEPLEPAWKRSAINVPSPG
                     TIASYRKGQYHAHETVSEYHVHMDRYDPEKNPIMHLIDDAPLLLMIRETMETIFVTGR
                     DALQRDPLQRLADQRMSWRLRICLGAVLLAIGAILLLLVIGKTVLFFAVILPSLVLIL
                     GLLTIVEGIRGREHREHAGKKVVQGGILAGAGLVLFAFWELYLVLILLTLMVWFLSSA
                     AVTLLRAVRERRRLTKGFGYTLGLGMSSLALGILAITLPRGLVEILLTLLAGLTLMAG
                     AFTLLDAWGLRNAARLMEAGGGFQ"
     gene            complement(10631..13672)
                     /locus_tag="PLI76_02955"
     CDS             complement(10631..13672)
                     /locus_tag="PLI76_02955"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOI61020.1"
                     /translation="MPEQRKKSIKAGIFTLNPHTFCITRATDGISGMLGYAPEELASF
                     TLSDIWPDKVERERFARSIGGQNAVTDVSADLIRRDGELLPVTIAAASLPDTDITCIV
                     SARHPGAWEFPGGEEYLEVFDSLIDAVIILDRDGDILFANTAVATLTGVSSPAELIGQ
                     NPLQFAHPDYKEAVVRNLANLCSGHGGRLIHYPMQDLHGNERWVEALGTKIPFKGRDA
                     NLVVLRDITERKSVESELARTNQRFRDLYRLVRMMCDNVPDLIWAKDTERRYLFANRA
                     ICEKLLNAADTSEPLGRTDMFFAGRERRSRPDDPTWHTFGEICRDSDAIVMESGKAER
                     FDEYGNVRGKFLFLDVSKAPFRDDDGRMIGTVGCGRDVTEERRVEERLRWNEALLSLM
                     AQASPIAYLVVDNRTDDIHYFNSRFCDMWGLSSLEDDMRTGKIRNSDIIPPCISLIRD
                     PETFTACSSSLQDEANRLTVEDEVELVDGRCIYWFSTQIRDTDDRYLGRLYLFEDITE
                     RKNASEALRRHDAIMGAVNFAADRFLKESDWKRQMQEVLERFGRATDVSRVYIFENGT
                     DPATGNLACSQRWEWAIEGAAAGIDNPDLQSIPYEEFPRWCRKLAAGRPIVGPVWTLP
                     DNERVYFESRQVQSIAVVPIFIHQQWWGFIGFDEDRRERIWSPAEIEALQAAASIIGS
                     AILRTMNEEVYRNPVERSPMGVYLMQDGYMRYFNTRFADIFGYSRDELHGCAAQFPLV
                     APECRRDLQERYQALLSGDTESEHHEFRGMHKDGRVLYLENFATRLWFEGRPAISGSL
                     MDVTDRKQADEALRSSEERLKVLFDYAPDAFFLTDMHGILIDGNRAAERLAGCPKEEF
                     IGKSIVEAGFVPHEEYFKVEAMLNRLAAGTTAGPTEFFPVRGDGTRISIEAMAFPVTI
                     DGQSLVLISARDTSERHQLENLKKKAFLQIEENVEQLAILNDTIRNPLTVIIALAEMG
                     GTEIDRKIIKVAWEIDAIISRLDQGWLVSRKVKEFLKKHYA"
     gene            complement(13831..15141)
                     /locus_tag="PLI76_02960"
     CDS             complement(13831..15141)
                     /locus_tag="PLI76_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOI61021.1"
                     /translation="MESIVFRTMAAIIAIVAIAAAGAVSAQPNPSLHLCVAAEENAVW
                     PGGGTGIPPDDRSAESVVTGSNRFACNLYRRLADDSGSENLLFSPYSISSALAITYEG
                     ARGTTADEIRSVLHLPANDTLRREGFSRLDAGLKVAGANCTVQTANALWAEKTYPFLP
                     EYIALAERWYSANAMNLDFASAPDASRQAINRWVEGRTGGKIRDLLPPASIDPMTRLV
                     ITNAISFKGTWAGPFDADWTEEAEFRVGANETVEVPMMHGDAVYPYGETATLQVLELA
                     YAHENGTGLSMLILLPKGGSLAAVEEALDPQNLSTLRRSLTRERIDVYLPKFTLDASY
                     RLPSTLAAMGMPAVFSPGADLSGMDGTKNLFISGAVHKAFVDVNEEGTEAAAATGFAG
                     NASAAPPGGGIPVFRADRPFIFLIRDDGSGSILLMGRVIRPVAA"
     gene            15461..15913
                     /locus_tag="PLI76_02965"
     CDS             15461..15913
                     /locus_tag="PLI76_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HOI61022.1"
                     /translation="MRRFVEWFERIVYITLIAFLVVLLTFTLLELGWLLVIGLFEDSI
                     YRLESHELFNLFGYFLLILIGLELLETIKAYLSRREFHVEIIILVAIIAIARKVILIS
                     TATPGELIGIALIIIALCSGYYLVHRAGLPRSLPPEDPPSALESREEE"
     gene            16472..19486
                     /locus_tag="PLI76_02970"
     CDS             16472..19486
                     /locus_tag="PLI76_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOI61023.1"
                     /translation="MISILYVDDEPDLLELARIFIERTGEFRVATSTSALRSLADPAI
                     RSYDAIISDYQMPGMDGIAFLKAVRHQIGDMPFILFTGRGREEVVIEAINNGADFYVQ
                     KGGDPKAQFTELAHMIRQAVRGRQAKMTLAEQEQRYHDLQNANDLIQSVTPDGHFLFA
                     SKKWLETLGYTAEELPNLTVFDIIHEESIDHCMTIFQRVLSGESVGIIDAIFKTRDGK
                     KVYVEGIADGKMADGRCQYTRGLFKDITERVQAEKALCDSEHRFATIFKNSPIMLTLV
                     SAIDGTLVDVNDAFLRYTGYDREEVIGRKVEELGLFPDRPGYLRFITALRAGRPVEGM
                     RMLCRGKSGDVHTCRFFSNMTLINGRPHTLSAVEDLTEQKRAEEAARESEERYRLIME
                     NAGEGILANELTPDGPGRFIDVNDAACRILGMTREELQGMKLIDFDTLEVKRRAPALV
                     QELMQKKRVVFQAGFRTGGDREKTLDISVSLFELTGKPAILSIIRDITEVEATRSALH
                     TLVAGMVGTTGRESLDRIVESLSAWSGANCVLISEIMPDREHARVLSMLLDGKKIPEH
                     SYTLKGTPCESVAKGGFTVYPDNVAELFPESQGVRELNIRGYAGAPLRDSAGRVVGIL
                     CIVSRTPLALLPSAQEIIEIVASKAAAEIERMNALSSLSESEEKFRALVEHSLDGTLI
                     LDPAGTILFANHAAGRLLGVDHPDELIGGRSIMEFVVPASQNEIINDFGEVAMWVDGY
                     IARYKILTANQGERLVEGIGKSILFNGAPSILISLRDITGRWRAENALREANRKLNLL
                     SSITRHDINNQLQILNGYAMILRDINHDPSLDGYLSRVATASSRIASMIQFTREYEEI
                     GVRLPVWQGLRTLVDEAGRGANPGGVVLENDMPEAMEVFADPLIGKVLFNLIDNALRH
                     GGKVTTVRFAATIRDEACIVTCEDDGDGIAPGLKERIFDRGFGKNTGFGLSLSREILD
                     ITGIAIRETGEAGRGARFEIAVPKGQYRAGPD"
     gene            complement(19621..20592)
                     /locus_tag="PLI76_02975"
     CDS             complement(19621..20592)
                     /locus_tag="PLI76_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61024.1"
                     /translation="MSRIAPAVILFFLAPFVAEFLLGNLPITMLSSLIVLAPMYGGGA
                     LLIREVVRRTSRGWPGMIVLALAYGVLEEGITTQSLFNPNYLNAHLLDYGFLPSLGIA
                     IPWTIFVLTIHTVWSISVPIALVEVLFSKRRTTPWLGRPGLALTCILFAIGIIATTAI
                     TLSTDAFIAPVPQLAGAVIAIIILIIIAFSLKPGEPFDTDTHPAPNARSVGVFALIAS
                     SIFMLIPHPPAWWIPVVLYVILGIVVFIVIRSWSRRRGWGDWHRLALAGGALLTYAWH
                     GFPQPSVFLGNSTVDLIGNAIFAAAAVILILIAARRLQQAGSAPLPA"
BASE COUNT         3785 a         6354 c         6590 g         3973 t
ORIGIN      
        1 gaagacgttt ccccggccgg agaggcgctg gagtatgaat ccctcgccgc cgaagaaacc
       61 cgaccggagg cgcttcgtga acgcgatatc gaggtcgatg cccttttcgg agcagaggaa
      121 ggcgtctttc tgggcgatga actcacctcc gtcaaggttg agcgcaaata tttttcccgg
      181 gatgttcccg gcaaacgaga cgaacccccc gccgccctgc ggggtgaact cggtcatgaa
      241 gaggccttcg ccggtcacca tccgcttcaa ccccttgaag agaccgccct tcatatgggt
      301 cgtcatctgc atgttcccgc tcatgttgac catggcgccc gcctcggcgc aggcgctctc
      361 gccggaggcg aggcggaggg tcaccatctg gaggttatct ccggtgatct cgtattgcat
      421 accggaactt ctttcatccc cgggcaatag gtcttttctc tctgttctct ccgggccggc
      481 ggctctgttt ccgaaatccc cctccggagc ggtggcttta accgggatca atgccgtaac
      541 gatttgtatg gagaatggat cgtttagcat cctccctgcc ccgggcatac gctccggtac
      601 cgtcccggcc gggagggtgg cagtatgaga ccgatcgttc tggttctgct tctcattgcc
      661 gtcgcagggg tcgcgggaac ggaggcgatc aacgtctcgc tgcccgaccg cgacgcgccg
      721 gaggccgtgc tcgggacggt cgagtcgatg tgggggtttg tcgaatggct gctgcaggtc
      781 gccgacgacc tgctctcgct catccgtaat gtgctcgggt ctctcggggt gggaggcgag
      841 gaggtcgaac gccttatggg ggcgatggac tcgggaaagg atctcgtgaa caggaccctc
      901 gaagagggtg cgccatccgg gaggggttaa tcgggcagac gctccctatc cttctcgccg
      961 gtgcgccggg tcaaacgtcc cgctcagcac cgggtcgacg cactttgacc tccgcgagga
     1021 gacggtctcc ggcgaatcgc agacgccggg gagactcgag gccccctgcc cgtcaccaga
     1081 accgcatgcg ccggttccgg caacctccgc gtcctctcac acgagtgtaa gtaggatccg
     1141 gtctgttgcg ctgctgtttt cttccccggt cgtctcaaaa aatgggtggg ccagagagat
     1201 ctcatgccca tccgggcatg gggagggggg ggaggaggac ccgtcaaggg gcggggggag
     1261 cagttctacg gcgaacgaag agacccggat ctcgtccgtc gcggggacgc tgttgccctg
     1321 gatgacgctt ccagggtcct ccttcatggg cagggtcaca ggtctccagg tcgatgacct
     1381 cgtcgggatc aaggatgata ccgacgccca cgccggccat ggcggcggcg cagcgctcgt
     1441 ggggagcgag tcgagaacca ggacccgtgg ggtcgtgctc gtcggtcctg ctcaggcgct
     1501 cggcacgacc ctcgtgaggg ctctggatcc ccgggctgcc gttgaaggct ccgggagacc
     1561 aaaaggccat cgcaagattt atccgccagg ggggcatctc cccgcgtgat catcatgcgc
     1621 ggaggagagg aagcggcgca tcctgcagag gcgatcaccg cgggagcgca cctcacggat
     1681 gaggcccggt cggccgccct cgagcggcag tgccaccggt gtgccgggtg caacgcaccg
     1741 ctcactgccg gttccacgca ctttgatatc aggccgccgg tgatctgcgg cgggtccccc
     1801 ctgcccggga accttgaggc cctctgcccc ttctgccacc agaacttcat gcgccgggtc
     1861 cgggtgcgcc ttgccggcca taggaataag tagtacccgg ggtcataccg ccgatcatga
     1921 cctgcagacg gcatgcagcg cagagcgacg agtggcgatc gacggtgatg aggagggcgg
     1981 atgagatcgc ggggaggctc gcgctgcctc catcgcccct tcccgggggc tggcgttccg
     2041 gcacgatgca gatgctcgat gccgccgtca ccccggggac ggtgcaaaac atcgcggcct
     2101 ccctcgcggt ggcgatgttc gtgatccccc gcataccgtg ccggcccctg gcgatcgccg
     2161 ccgggtttgc cgcgggctcg atcttcggga agtgaacctc aggggctccg cctcttcccc
     2221 cgggccggga cgagacactt ccacgcgctc ccgatcaggt cctcccgccc ggtcgcacgg
     2281 agcccttcgc ggacgagggc gtagtttgcc gggtcccggt agtgcatgag cgcccgctgg
     2341 atctgtttct cccggccttt cgggacgtag acctcctcga gggtgaaggg gtcaagcccg
     2401 gtgtggtaga tcgtcgtcga tatgctcatc ggcgtcggcg tgaagtcctg gacctgttcg
     2461 gtgtaaaggc cggtgtcccg gacgtactcc gcgagttcga ccatatcctc catccggcag
     2521 cccgggtggc cggatatgag gtaggggacg aggtactgcc gtttcttctt ccctgcctgg
     2581 aggacctcga accgttcgcg gaaggcatcg aagaccgcgc gggggggttt acccatgcag
     2641 gccgtgaccc gtgccgagac gtgttcgggg gcgaccttga ggtgcccgga gacgtggtcg
     2701 gcgcagatgc gggagagata ctcttcatcc tccgggccga tcaggtcgta gcggatgccg
     2761 gatgcgatga agacccgtct cacccccggt atatccagga ggcggcggag caggcggagc
     2821 tggtcggcgt ggctccggtc gagcgcaggg cagtcgatac agcggcggtc gggacaggtg
     2881 ccggcggtct cccatcggcc gcagtgaatg ccgtacatgt tcgccgtcgg cccgccgata
     2941 tcctggacga tgcccgtaaa ctccggcatc tttgccatcc gctccacttc gcggacgatc
     3001 gagtccgcgc tccggctctg gatgatcctt ccctggtggt gggtgagggc gcagaacgaa
     3061 caggcgccga agcatccgcg gtggctgacg accgagaacc ggacgggttc gagggccggt
     3121 acgggttcgg cgtaggaagg gtgcgccctc cgcgtgtagg ggagttcgta gacccggtcg
     3181 agttcaggcc ccgatagcgg tatcgccggc gggttctgga tgacgacggt ctttgggtgc
     3241 ggctgcgccg ccggccggcc ccggaaggga tcctgctcgt tgtagtgcag ggcaaacgcc
     3301 cgtgcatagg cgtagcgatc ggatttgacc tccgcgtagt ccgggaggac gacgtacccg
     3361 gtcgggtcca tgccccgcca cgtcttcctg tcgacccggc aggctgtgcc ggggatatcc
     3421 gtgagggttc tttcaccggc ggcgaggcgg tccgctatcg tgaccacctg gcgctcgccc
     3481 atgccgaaga cgaggagatc ggccggcgcg tcggcgagga tggactggcg gaccgagtca
     3541 gaccagtagt cgtagtgggc gaaccgccgg aggctcgcct cgatcccgcc caggacgatc
     3601 ggcgtctccg ggaagagggc atggacccgg tcggtgtaga cgagtgtcgc ccggtcgggc
     3661 cgcagaagcc ttccgccggg cgagtagacg tccgaactcc ggcgtttcag gttcggcgtg
     3721 aaggcgttca ccagggaatc gacgttcccg gccgatatcg agaagaagag gcggggctcg
     3781 cccaggcgcc ggaggtcggc gtcactgcgc cagtcgggct gggcgatgac gccgaccgag
     3841 tagccggcgt tccagaggac ccggccgacg atagcggtcc cgaaggaggg gtggtcgacg
     3901 taggcgtcgc cggatatgag gatgacgtcg aaccggtcga tgccgagccg atctgcctcc
     3961 tcccccgtca tcgggaggaa ctccggctgc ccgctcatgg gaggaacccg tcgaagacag
     4021 gcaggtcgac gccggtcttc tcgtccggga gcctccgcga ggtgatcccg acgaactccg
     4081 tgatgatcgc ttcgacgacg atgtaaactt ccgccttatc gcggaggcat ccggtgagcg
     4141 accgtatccc cctccccgcc gtggcgtgga tgtggagaga cggtccatcc tccccgggat
     4201 ggaccgtcgc gaacccgagg agttcccagc ccccgaatat ctcctcgcgc cgcggtgtcg
     4261 gcggcacgac cggttctttc ggtcccgtca ccagcgtcgc ggtccggacg gcgccgagaa
     4321 agtggaccat accgctctgg acgttcatcg ccgccacgaa ccgccggatc tccttgatga
     4381 gatcctctcc atcgtcgacc ctgaccgtaa agacccggcc gacctgccct tccgagtact
     4441 gcatggactc actctccagg taggtgatgc ggcatgataa tatagtgccc gggcgaggga
     4501 ttcataagcg ccggcctcgc aatgcgagat atggtcgcgg ggatctggtt ccgggaggtc
     4561 gaatacctgc gggggtttgc agctcttgcc gtgatcgcgg tccacgtctc catgaacttc
     4621 acccggctcc cgggggcggg gctgcccgcc ctcgtcggta tattcgtcta cattgccgcc
     4681 cactttgcgg tgccggtctt tgtcttcata tcgggctggg tgctcgcggc ccgataccgg
     4741 ggtggttacc ccgtcccggc ctactaccgg cgccgtgccc ggacgatcct ccccccatat
     4801 atcctcttct ccgccctcta cctcccgata gtcgtggagg gggcaatggc gtttgccggc
     4861 atgcccgctc ccggggcaat ggtgggcgcc ctcctcatgg ggaccgctgc ctaccacctc
     4921 tggttcttcg tcgtgatcat ccagctctac atcctctacc ccctgatcgc cggggtctat
     4981 gaccgcttcg accgggccgg ggcggcgctc ttcctcctcc tcgccctcct cttcgtccag
     5041 gtactctgga acgtgggcgc ccatgccgcc ggggcgtttg caggcacgga atggtatgcg
     5101 gtgctgatcc gcctctttcc gtcgcacctc ctctacttcg ttctcggcgt ccacgtcgcc
     5161 cgacatacag atcggttccg gtcgatgccc ggatcgctct ccccggcgtg ggtcgtcgcc
     5221 gccgcgggtg cgggggctct cctcaccgga gggatctggg tcgcggccgt ctatctctcg
     5281 ggcgggtttg ccggcgcaac gctcggcgtc ttctgtgtct accggatcat cgaaccgttc
     5341 tactacgtcc cggtcatagc cgctctcgtc gttgcagccc ggcggctgga gaggggcggc
     5401 ggccgccctg cgggggcttt ccggtcgttt ggagcgcatt cgttcgggat ctacctcatc
     5461 caccccctcg ttatcgcggc cggcgcggcg gcatgggccg cccttacggg atggacgtgg
     5521 gcggactggc cgacctaccc ggtgctcttc gtggtggcgg cggcggcgag ttacggggcg
     5581 gttcgggcgg catcagcggt cccgtatgcc gggtacctgc tcggggagcc gcgagcgttg
     5641 cccgcaacgg agcggtcgtg aaccgcagga gaggagggca cactcaagtc cacgtgtgcg
     5701 aagacccgga ttgcctcacc attctggcgg tgcgggcccc ggccaacgaa gaatctcgcg
     5761 gctccggccc gtggagagcg cccggtgcca cggcacccgg acccaaccat taagtgatct
     5821 caggtttatc gggggccgca gccccggctg cggatggtga agagaatatg ccagagcaga
     5881 taagcacgcc ctcgtatcag gtgtatacga aaggcatcga ctaccccaga tccaaacagg
     5941 aggtcatatc ctacgtccgt gaacacaacg cccccgatga cgtgatccgg gtcatggagg
     6001 agatgcagga ccagcagttc acatcggcgt cagatctcgc agtagcgttc ggcgaggcaa
     6061 aagaccggct cgaagggcgg cggtaacact ccgcaccctt tcccgggtgg agcacacccg
     6121 ctcttttaca ggccccggaa ccatcgatcg cggcaatctc gcggcctgcc ggcgaccgga
     6181 cgggacttcg cggcaacgcg cgagctacct ggtgtggata gcaagacaat ctctcgcgcg
     6241 aagccgcgaa gccttgatgg ggagttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnttcg cggcaacgcg cgagctcagg tgcataaacg atacggcctc ccgcgcgaag
     6421 ccgcgaaggt gcgaagggga gttccagacg tccgggcggg gataaccaga agatctcccg
     6481 cgctgccgca aaggggcgtg gaacagagat cttgccgggc aggccggatg aagggatgcc
     6541 gtgaaaccgc tctgctcata gtccggcatc agaaaatatg gcacccgtcc tctctctcgc
     6601 aacacgtttc cagtaatctg ttgtggtagc accgtcgaac gttgcgcacc ggttgcgcga
     6661 tgcctcctca acggccgcag taacgtttga ttgacccacc ttccgtgctt cttcacacct
     6721 cccgacggat aacagactca tgtctctgtc gaacatgcat ccgcatccct cccgtggtcg
     6781 catgccatct tccgttttgc cttgtggcag gatgacgtga tcgtcaggct caattaagaa
     6841 tttgcacaac aatattgcca tgcttatctg caacgccctt ctgcacaccg gatatgcaat
     6901 gcatagctcg cgggggtcat gtggttactc atgacacctt caaccgcttt cttacccgac
     6961 agtccctacc cccggagacc atctcgccag gagcgacctc gtcccgttga ataatcgagt
     7021 cggtcttttg gctctcaggt tatcccgctt cggttcttcg tcctgggcga tgtctgcctt
     7081 cccggatagc atccgctctt tctctgtcat agttgatctc ccggcaggga gagatccgaa
     7141 tgtatccggc ggggcggatc gggaggcccc taccccgggt gaggtcgcga ggggcgtctc
     7201 tccggcgcga caggcgcacc gggcgacggc gacgtcacac cgcttccgaa ccgaaccgga
     7261 acgcccggga gggcacccgc atctcgaacc gtgccccctc tccaggttcc cctctctcgg
     7321 cgatggtgat cccggtgatc gcgaggatct cccggatcag gaagagcccg agccccgtgt
     7381 tcttcccgaa tccacggtcg aagatccgtt ccttctcccc tgcaggaacg ccgacaccgt
     7441 cgtcctccca gacgagggtg aggtccccgt tctcctccgg acgataccgg agatggaccc
     7501 gggtcgccga ctcggcgtgc cggatcgtgt tgtccatcag gttggcaaag accttcacga
     7561 gcatcggatc cgcgtagatt gcaaggccat ccgtctcatc ggttatcgga agtcccccgg
     7621 ccgccgcagt ccggatcgcg cccgatacgc gttgccattc cggagcacgg acgccgaggt
     7681 cctggtagtc acgggtgaac tcgatctggc gctggatggt cccggcggcg atgctcatct
     7741 tctcaagata tcctgagagg ggtccttcgg acacttcctc ctgtgcgaac gagaggttgc
     7801 cgaggaggac cgtcagctgg ttgaggatat cgtgccgggt tatgctgctc atcaggttga
     7861 gctgccggtt cgcctgccgg agcgcctgtt cagccaggtg gagatcggtg acgtcgtgga
     7921 tgatcccttc cacgccccgg accgaacctt ctgcatcggt gaaaaaatgg ccgttcaccg
     7981 tcacgtgctt cagagttccg tctttcatct tgagcaccag tggatatccc ccgaccgcgc
     8041 cgttcgccct gagcgcgacg aggaacctct cacgttccag aggatcggca tagagttcct
     8101 gcatggtcac tccgatcatc tcttcgggcg aaccgtagcc tgccagtgcc accccggacg
     8161 gactgatcat ggtgatggtc ccatcgagat cggtgcggta gaacagatcc tgcatgttct
     8221 cgatgatggt ccggtatttt tcttcgctct ctttgagggc ctcttctgcc tgcttgcgct
     8281 cggtgatatt ctggagaagg accagttttc cgatgaccgc cccggtcccg gtccgcagat
     8341 cgtcaacggc tacatcgaag aactgcctct ccagtccctc tccgatggcg atctcccggt
     8401 tatccccgga tgcgccctgt gccagggcaa ggaccgaggg gtggcccgcg aggacgagat
     8461 ccgccgggct gccgatggca tcgtcatgca ccgtcccgag gagccgaacc gctgcggtgt
     8521 tcatatcggt catccgcccc cgcgcatcga tcaccagcat cggcgagctc atctcctcga
     8581 tcaccttccc ccgggcgacc ggggctacct cgaggaggcc ctgtctgagc gttgctccca
     8641 caaagacgag gccggtgaac atcagggcga acggcgtcaa cgtcagtccc gggtacgggg
     8701 tgaggttcag ctggtactgg agatcgcaga ggagggggag gagaagcgcg gccaggagaa
     8761 ggaggcgctg gcgccggtag aaggcatgga tctccggctg gacgaatatg aggaggagga
     8821 gactcgcgat caggaggatg tgagagtatg caacatggac ctggaatcct attccgttgg
     8881 tgaagttgag tatgggcaac ggtcccgaga ggtcgaccca gtaatcgtac cggtagagcg
     8941 ggtgaaacca ggtggtctgg gcgaggatga cggtcattat cgggatgatg aagagggaga
     9001 cttttttcca gccctgtatc cactcctccc ttctcaggta ggtgaggatg aatatgagtt
     9061 gaaagacggc gaggaacggc acggccagga accggatctg cagcaggaag atcttgagcc
     9121 agagcgtctc cgtcatcagg ttgagtgcat gggtgatggg caggaaggcg gcacacaaca
     9181 gcaggagtcc gaggggccgt gccagtgcta tgtcgcggtg cttgtagcaa tagagcatga
     9241 gggcgatgag cacccccccg gcaaggaaga ggagcccgat caggagggcg agtatcatgg
     9301 acagtctcta ggggtagtgt tttgattgac ggatgtttaa tgttgcgttt aggaacaggc
     9361 gatgaaagcg cagatccggg gaaaagagcg ggtttaggag cccattcaat taaataaacc
     9421 taattataag agtttgagga attcgcgttg ataggtgcaa gccggggcgc tctcgctccc
     9481 gggacacccg gctggagaat acgtgtcctg ccgctgtctt ttcccggatg ggaatctgcc
     9541 tgaccggcgg ggttgccccc tcttctacga gtaactccac ggcgacgaag cgtaccggac
     9601 cctcacattt aatacaacct cctccagaaa gtgtgatatg cgcatctaca ggcctcgtat
     9661 ctcaagagac cttcccgcgg gtgtcggcga atcctgagaa cggctgagga ggagatgggt
     9721 tgagcacgct tgagtcgtat cttgatgcac tgcaccgggg ggtcttccag gtcgtcgtac
     9781 caaaggccct ggtgaccgaa ccgctcgaac cggcctggaa acggtccgcc atcaatgtgc
     9841 cgagcccggg gaccatcgcg agttaccgga agggccagta ccatgcccac gagaccgtct
     9901 cggagtacca tgttcacatg gaccggtacg accccgagaa gaaccccatc atgcacctga
     9961 tcgacgacgc cccccttctc ctcatgatcc gcgagacgat ggagaccatc ttcgtgacgg
    10021 gcagagatgc gctgcaaaga gaccccctgc agcgcctcgc cgaccagcgc atgagctgga
    10081 gactccggat atgtctcggt gcggtcctcc tcgcgatagg cgctatcctc cttctcctgg
    10141 tgatcggcaa gaccgtactc ttctttgccg tgatcctgcc ctccctcgtg ctcatcctcg
    10201 gccttctcac gatcgtcgag ggcatccggg ggagggagca cagagagcat gcgggcaaaa
    10261 aggtcgtcca gggtggtatc ctggcaggcg caggccttgt tctattcgcc ttctgggaac
    10321 tctacctcgt cctcatcctg ctgaccctca tggtctggtt cctctcgagc gccgccgtca
    10381 cgctgctgcg ggcggtccgg gagcgccgaa ggctgacgaa gggttttggg tataccctcg
    10441 gcctcgggat gagttcgctt gcgctcggga tcctcgcaat caccctgccc cggggactgg
    10501 tcgagatcct tcttaccctg cttgccgggc tcacgctcat ggccggcgcg ttcacgcttc
    10561 tcgatgcgtg ggggctccgg aacgccgccc gcctgatgga agcgggcggg ggcttccaat
    10621 gaccatcctc tcacgcgtaa tgcttcttta aaaactcctt cactttcctt gagaccagcc
    10681 atccctggtc gagccggctg atgatcgcat cgatctccca ggccaccttg atgatctttc
    10741 gatcgatctc cgtgcccccc atctccgcaa gagcgatgat gacggtgagg ggattgcgaa
    10801 tggtgtcgtt taagatggca agctgctcga cattctcctc gatctggagg aacgcctttt
    10861 tcttgaggtt ttccagctga tgccgctctg aagtgtcgcg agcgctgatg aggaccaggg
    10921 actggccgtc gatcgtcacc gggaacgcca tcgcctctat ggagatgcgg gtgccgtcgc
    10981 cacggacagg gaagaactcg gtcgggccgg cggtggtccc ggccgcaagc cggttgagca
    11041 tggcttcaac cttgaagtat tcttcatgcg gcacgaatcc ggcttcgacg atgctcttgc
    11101 cgatgaactc ttccttcggg cagcctgcca gcctctccgc cgcccggttg ccgtcgataa
    11161 ggatcccatg catatcggtg aggaagaagg catccggggc ataatcgaag aggactttca
    11221 accgctcctc cgaactcctc aacgcctcat ccgcctgttt ccggtcggtc acatccatca
    11281 ggctgccgct gatggccggt cgcccctcga accagaggcg ggttgcaaag ttctcgaggt
    11341 agaggacccg cccgtcttta tgcatgccgc ggaactcgtg gtgctccgac tcggtatcgc
    11401 cggagaggag ggcctggtac cgctcctgca gatctctccg gcactccggg gcgaccaggg
    11461 ggaactgtgc ggcgcaaccg tgcagctcgt cgcgcgaata cccgaatata tcggcaaacc
    11521 gggtattgaa gtagcgcatg tagccgtcct gcatcaggta gacgcccatc ggggagcgct
    11581 ccaccgggtt ccggtagacc tcctcgttca tcgtccggag gattgccgaa ccgatgatgc
    11641 ttgccgctgc ctggagcgcc tcgatctccg ccggcgacca tatacgctcc ctccggtcct
    11701 catcaaaccc gatgaagccc caccactgct ggtggatgaa gatcgggacg accgcgatgg
    11761 attggacttg ccgggattcg aagtaaaccc gctcgttatc ggggagcgtc cataccggcc
    11821 cgacgatagg tctcccggcc gcgagtttcc tgcaccagcg gggaaactct tcgtagggga
    11881 tactctggag gtccgggtta tcgatccctg cggcggcccc ctctatagcc cactcccagc
    11941 gctggctgca ggcgaggttg ccggtcgcgg gatccgtgcc gttctcgaat atgtacaccc
    12001 tgctgacgtc ggtggccctg ccgaaccgct cgagaacttc ctgcatctgt ctcttccagt
    12061 ccgactcctt gaggaacctg tccgccgcaa agttcaccgc gcccatgatg gcgtcatgcc
    12121 ggcggagggc ctcggatgcg ttcttccgct cggtgatatc ctcaaagagg tagagtcttc
    12181 ccaggtaacg gtcgtccgtg tcgcggatct gggtggagaa ccagtagatg catcgcccgt
    12241 cgacgagctc gacctcatcc tcgacggtca accggttcgc ctcatcctgg agtgaggaag
    12301 agcatgcggt aaatgtctca ggatccctga tgagcgatat acagggaggg atgatatcgc
    12361 tgttccggat ctttcccgtc ctcatatcgt cttccaggct tgagagcccc cacatatcgc
    12421 agaaccggct attgaagtaa tgaatgtcgt ccgtccggtt atcaacgacc agataggcga
    12481 tcggggaggc ctgggccatc agggagagca gcgcttcatt ccaccggagg cgctcttcca
    12541 cccgccgctc ctcggtcacg tcgcggccgc accccacggt cccgatcatc cgcccgtcgt
    12601 cgtcccggaa cggggccttg ctgacgtcga ggaagagaaa cttccccctg acgttcccgt
    12661 actcgtcgaa ccgctccgcc tttccgctct ccatgacgat cgcatcggag tcccggcaga
    12721 tctctccgaa ggtgtgccag gtggggtcgt ccgggcggga tcgtcgctcg cgcccggcaa
    12781 agaacatatc cgtcctgccc agcggttcgc tggtatcggc agcgttgagg agtttctcgc
    12841 agatggccct gttcgcaaag aggtatcgcc tctccgtatc ttttgcccat atgagatcgg
    12901 ggacgttgtc gcacatcatc cggacgaggc ggtagaggtc gcggaaccgc tggttggtcc
    12961 gggcgagttc gctctccacg ctctttcgct ccgttatatc gcggagcacg acgaggtttg
    13021 cgtcccgccc tttaaagggg atcttcgtgc cgagcgcttc gacccatctc tcgttcccgt
    13081 ggagatcctg catcgggtag tggatgaggc ggccgccatg cccggaacac aggttcgcga
    13141 ggttccggac gacggcctcc ttgtagtcgg ggtgggcgaa ctgcagaggg ttttggccga
    13201 tgagctcggc cggcgaagag acgccggtga gcgttgcgac cgcggtattg gcaaacagga
    13261 tgtcgccgtc ccggtcgagg atgatgacgg cgtctatgag ggagtcaaag acctccaggt
    13321 actcctcccc tcctggaaac tcccatgcac cggggtgtcg ggcggagacg atgcaggtga
    13381 tatccgtatc cgggagggag gctgcggcaa tcgtcacggg caggagctca ccgtcacggc
    13441 ggatcaggtc ggcggagacg tcggtgacgg cgttttgccc gccgatcgac cgggcgaacc
    13501 gttcccgctc taccttatcc ggccatatat cggagagggt gaaggatgcg agttcctccg
    13561 gcgcgtagcc gagcatgccg gatataccat cggtggcccg ggtgatgcag aacgtgtgcg
    13621 ggttgagggt gaaaataccc gcttttatgc ttttttttct ctgttcgggc atccgggttt
    13681 ccttttccat cgtctttcaa gagatctcta tctcacgcct tatactggta tctatttcct
    13741 ttcgggccgg acaaccaggg cgcgttctcg cggcttcggc tcaagaatcg cccgccgtac
    13801 cggtaaagat gacccgaccg cccgggaagg tcaggccgcc accggccgta taacccgtcc
    13861 catgaggagg atgctcccgc tcccgtcatc ccgtatgaga aagatgaacg gacgatccgc
    13921 cctgaataca ggaattcccc cgcccggagg cgcagcactc gcgttcccgg cgaaccctgt
    13981 cgccgccgcg gcctcggtgc cctcctcgtt cacatccacg aacgccttat ggacggcgcc
    14041 gctgatgaag aggttcttcg tgccgtccat tcccgagagg tcggcaccgg gggagaacac
    14101 cgccggcatc cccatcgccg caagcgtcga aggaaggcgg tagctcgcat cgagcgtgaa
    14161 cttcgggaga taaacgtcga tgcgctcacg ggtgagcgag cggcgcagcg ttgagaggtt
    14221 ctgcggatcg agagcctcct caacagccgc aaggcttccg cccttcggga gcaggatcag
    14281 catcgagagc cctgttccgt tctcgtgcgc ataggcaagt tcgagcacct ggagggtcgc
    14341 ggtctctcca tacgggtaga cggcgtctcc atgcatcatc gggacctcca cggtctcgtt
    14401 cgcgcccacc cggaactccg cctcttcagt ccagtccgca tcgaacgggc cggcccaggt
    14461 tcctttgaag gagatcgcgt tcgtgatgac gagccgggtc atgggatcga tcgaggcggg
    14521 aggcaggagg tcgcgaatct tccccccggt tcgcccttcg acccaccggt tgatcgcctg
    14581 ccgcgacgca tcgggggcgc tcgcaaagtc aaggttcatc gcgttcgcag agtaccagcg
    14641 ctccgcgaga gcgatgtact ccgggaggaa aggatacgtc ttctccgccc agagggcgtt
    14701 tgccgtctga acggtgcagt tagcccccgc gaccttcagg ccggcatcga gccgggaaaa
    14761 cccctcccgc cgaagggtgt cgtttgccgg gagatggaga accgaccgga tctcatcggc
    14821 ggtcgtgccg cgggcgccct cataggtgat cgcaagggcc gacgatatgc tgtaaggcga
    14881 gaaaaggagg ttttccgacc ccgaatcgtc tgcgaggcgc cgatagaggt tgcaagcgaa
    14941 acggttgctg cccgtaacga cgctttcagc cgaccggtcg tcgggaggta taccggttcc
    15001 accccccggc cagacagcgt tctcttcagc ggcgacacat agatgcagcg acgggttcgg
    15061 ttgcgccgaa acagctcccg ctgcagcaat ggcgacgatg gcgatgatgg cagccatcgt
    15121 cctgaatacg atagattcca tatggttcac cctcatctgg ctgttgccgg accttcgggg
    15181 gcccggtgcg tgcggccgcg cccccgtgca agtccccctg ccgttgctcc cgggtaccgg
    15241 cagcgccggg tgtatgcgca tatcctgcag attatacgcg aaacctgcag gtggtaaggg
    15301 tgagaggaca tacggggtcg gggttctcac gctgcggcgt gatggaggag agagtatata
    15361 gtaatgactg caccgccccc gggattccgc ccgttacggg gtctgaacgc acatatagtt
    15421 ttcggcagat atttatgcaa atcaaggcga taaaccatat atgcgccgtt tcgtggagtg
    15481 gttcgagcgt atcgtctaca ttacgctcat cgcattcctg gtagtgctcc tcacctttac
    15541 gctcctcgaa ctgggatggt tactggtcat cggcctgttt gaggattcta tctaccggct
    15601 tgagagccat gagttgttca acctctttgg ctacttcttg ctgatcctca tcggccttga
    15661 gctcctggag acgataaagg cctacctgag ccggagggag ttccacgtcg agatcatcat
    15721 cctcgttgca atcatcgcca tcgcaagaaa ggttattctc atcagcacgg caaccccggg
    15781 cgagctcatc ggcatcgcgc tgatcatcat cgccctctgc agcgggtatt accttgtaca
    15841 ccgggcgggg ttgccgcgct ctctcccccc ggaggaccct ccatccgcgc tggaaagccg
    15901 ggaagaagag tgaccacggc gactgctctt cctgctcaac cgggagcggc cgaccgctct
    15961 cgccggcggc ccttgcccgg caccgacagg ggatctgctc tctctccgct cagggccagc
    16021 tcctgtcgtg gggtcgtcga cggccacacc cgccacaaga gcagggtggt gtccaccgcg
    16081 ggcgttctcc gccatgaacc caccggcggg gcccggggag cgcatgccgg atcgcgcccc
    16141 cgccgatctc gatggccgga ccacccggat gtatcgatgc atcttgcaaa atccgccggg
    16201 agaagagaga tacgccggca aacaggctat tatttcaata tatcgcgtta gcagctctcc
    16261 cgtgagtaac cggggtgcga gataggtcgc ctgcggatat accctgatat ccggcgttat
    16321 cgggtatcga cagactgcaa cgcttataag gcgacgtggt gagagaggta gtatcgatcg
    16381 ccctggcggg ctacaggtgc cggcattctt tccctcacgg gaggcatgcc tgccctgcgc
    16441 gccgaccacg atctgcagga gaagtggtga tgtgatatcg attctctatg ttgatgacga
    16501 acccgatctc ctcgagctgg cccggatctt tatcgaacga acaggagagt ttcgggtcgc
    16561 aacatcgacg tcggcgttga gatctcttgc agatccggca atcaggtcgt acgatgccat
    16621 catatccgac taccagatgc cgggcatgga cgggatcgcg ttcttaaaag cggtccggca
    16681 ccagatcggg gatatgccct tcatcctctt caccggccgt ggccgcgagg aagtcgtcat
    16741 cgaagcgatc aacaacggcg ccgacttcta cgtccagaaa ggcggggacc cgaaggccca
    16801 gttcaccgag ctcgcccaca tgatccggca ggcggtgagg gggaggcagg cgaagatgac
    16861 ccttgccgaa caggagcagc gctaccatga cctccagaac gcaaacgacc tgatccagag
    16921 cgttacccct gacggacatt tcctctttgc cagcaagaaa tggctggaga cgctcgggta
    16981 tacggcggag gagctgccga accttacggt attcgatatc attcacgagg agagcatcga
    17041 ccactgcatg acgatcttcc agcgggtgct ctccggcgag agtgtgggga tcatcgatgc
    17101 gatcttcaaa acccgggacg ggaagaaagt ctacgtcgag ggcatcgcgg acggcaagat
    17161 ggcggatggg agatgccagt atacccgggg gctcttcaag gacatcaccg aacgtgtgca
    17221 ggcggagaag gcgctctgcg actcggaaca caggttcgct acgatcttca agaacagtcc
    17281 catcatgctc acccttgtct cggctatcga cggcacgctc gtcgacgtca acgacgcgtt
    17341 tttacgctac accggatatg accgggaaga ggtgatcggc aggaaagtag aggagttagg
    17401 gctcttcccg gatcggccgg gatatctccg gttcatcacg gccctccggg cggggcgccc
    17461 cgtcgagggc atgaggatgc tgtgccgcgg caagagcggg gacgtccata cctgccggtt
    17521 cttctcgaac atgaccctga tcaacggcag gccccacacc ctctcggcgg tcgaggatct
    17581 aacggaacag aagcgggcgg aggaggcggc ccgcgagagc gaagagcgct atcgcctgat
    17641 catggagaac gcgggcgagg ggatactggc aaacgaactc acgccggatg ggccgggaag
    17701 attcatcgac gtgaacgatg ccgcctgccg gatccttggg atgacccgcg aagaactgca
    17761 gggcatgaaa ctcatcgatt tcgatacgct ggaggtgaag agacgcgccc ctgcgcttgt
    17821 acaggagttg atgcagaaga agcgggtcgt cttccaggcc ggcttccgga caggggggga
    17881 ccgggagaag accctcgata tcagcgtcag tctcttcgag cttacgggaa agccggcgat
    17941 tctctcgatc atccgcgaca tcacggaggt cgaggccacc cgatcggcgc tccatacgct
    18001 cgtcgccggg atggtgggga cgaccggcag ggagtcgctc gaccggatag tggagagcct
    18061 cagcgcctgg tccggggcga actgcgtcct gatcagcgag atcatgccgg accgtgagca
    18121 tgcccgggtg ctctccatgc ttcttgatgg taagaagatc ccggagcact cctataccct
    18181 gaaggggacg ccctgcgaga gtgtcgcaaa gggcgggttc accgtctacc cggacaacgt
    18241 tgccgaactc tttcctgaga gccagggcgt ccgggagctc aacatccggg gctatgccgg
    18301 agccccgctc cgggactccg cgggacgggt cgtgggcatc ctctgtatcg taagtcggac
    18361 cccgctcgcc ctgctgccct ccgcgcagga gatcatagag atcgttgctt caaaagcggc
    18421 agccgagatc gagcgcatga acgctctttc gtcgctctcg gagagcgagg agaagtttcg
    18481 ggctcttgtg gagcactccc ttgacggcac gctcatcctc gatccggcgg ggaccatcct
    18541 ctttgcaaac catgccgcgg gccggctatt aggggtagat caccccgacg aactgatcgg
    18601 agggaggagt ataatggagt tcgttgtccc tgcatcacaa aacgagatca tcaacgattt
    18661 cggcgaggtg gccatgtggg tcgacgggta tatcgcacgc tataagatcc tcacggcaaa
    18721 ccagggggag cggttggtcg agggtatcgg gaagtccatt ctcttcaacg gcgccccgtc
    18781 gatcctcata tctctgcggg atattaccgg gcgctggagg gccgaaaacg cactcaggga
    18841 agcaaacaga aaactcaacc tcctctcgag catcacccgg cacgacatca acaaccagct
    18901 ccagattctg aacgggtatg ccatgatatt gcgcgatatc aatcacgatc cttcgcttga
    18961 cggctacctc tcccgcgtcg ccacggcaag cagccggatt gcgtcgatga tccagttcac
    19021 cagagagtac gaggagatcg gagtgcgcct ccccgtctgg cagggtctcc ggactctggt
    19081 cgacgaggca ggcaggggtg ccaaccccgg cggggtcgtg ctggagaacg atatgcccga
    19141 agcgatggag gtctttgccg acccgctcat cggcaaggtc ttgttcaacc tgatcgacaa
    19201 cgccctgcgc catggcggga aggtcaccac cgtccggttc gcggccacta ttcgcgacga
    19261 ggcatgcatc gttacctgtg aggatgatgg cgacggcatc gcgccgggtc tcaaggagcg
    19321 gatcttcgac cgggggttcg ggaagaacac cggttttgga ctctctctgt cgcgggagat
    19381 ccttgatatc accggcatcg cgatccggga gaccggggag gcgggcaggg gtgcacggtt
    19441 cgagatcgct gtgccgaagg ggcagtaccg ggccgggccg gattaaggcc ggcgacaacg
    19501 ccctccagag cggccacggt gcttcgtaaa gttccgggac accggaaagt gaggagatgg
    19561 tggttgcccc tcgccgggtg gagatagcgc cggcgacccg ggcaaccata tacccggcgg
    19621 ttatgcggga aggggggcgc tacccgcctg ctgcagcctc cgtgctgcga taaggataag
    19681 gatgactgcc gctgcggcga atatggcgtt gccgatgaga tcgacggtgg aattgcccag
    19741 gaagacggag ggttgaggga acccgtgcca ggcataggtg aggagcgccc caccggcgag
    19801 cgccagccgg tgccaatcgc cccacccccg gcgccgggac cacgaccgga taacgatgaa
    19861 cacgacgata cccaggatga cgtagaggac caccggtatc caccaggcag gaggatgcgg
    19921 tatcaacatg aatatcgagc tggctatcag tgcgaacaca ccgaccgacc gggcattcgg
    19981 cgccgggtgc gtatcggtat cgaaaggctc gcccggtttc aggctgaatg cgatgatgat
    20041 gaggatgatg atggctatca cggcgcctgc cagctggggc acaggggcta tgaaggcatc
    20101 ggtgctcagg gtgatcgccg ttgtggcgat gatgcctatc gcaaagagga tgcaggtgag
    20161 tgcaagtccc ggtcggccga gccagggggt ggtccggcgt ttcgagaaca gaacctcgac
    20221 gagtgctatc ggcacgctga tgctccagac ggtgtggatg gtgaggacga atatcgtcca
    20281 cgggatggcg atcccgagcg acggcagaaa gccgtaatcg agaaggtgcg cgttgaggta
    20341 gttcgggttg aagagcgact gggtggtgat gccctcctcc aggacgccgt aggcaagagc
    20401 aagcacgatc atgcccggcc aacccctact ggtccgccgg acaacctccc tgatcaggag
    20461 tgcgccgccg ccgtacatag gcgccagaac gataagggag gagagcatgg tgatggggag
    20521 gttgccgagc aggaattcgg caacgaacgg agcaaggaag aagagtatga ctgcgggagc
    20581 tatacgactc aagaacactc cgatggagtg ttgtgttcta cgagcagtgc tcgccgatcc
    20641 tctgccgggc atgatagccc gataacctgc atcgacttaa gaccttccgg caccggatcc
    20701 tcataccgta gtgagctgtt gccgacatca tggcccggtc tccgggcgcc acgggccccg
    20761 gtgctctgct accctggcgc ccggcctcga atgatgtgac ct
//
LOCUS       DAOSEE010000022        20473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1115_length_20473_cov_253.920413, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000022 DAOSEE010000000
VERSION     DAOSEE010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 20473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20473
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1115_length_20473_cov_253.920413"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /gene="aglJ"
                     /locus_tag="PLI76_02980"
     CDS             complement(<1..688)
                     /gene="aglJ"
                     /locus_tag="PLI76_02980"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer glycoprotein N-glycosyltransferase AglJ"
                     /protein_id="HOI61025.1"
                     /translation="MNIERDEVCIFIPTLNEAPTIAELIGEFKERGFRHILVMDGHST
                     DGTPDIARAAGAVVRTQTGRGKGNAIIEAVAIIDKPYVLMLDGDGTYAPDDAERMLDP
                     LGLGFDHVIGDRLAGPEGEAFTRLNLLGNQILNLMFKLAHGRDLHDILSGYRAFTLPS
                     IRQMTLKESGFEIETEMAVEAVRHGQRITVVPVRYLARPGTATKLNPLQDGARIFSTI
                     YRLAKMNNPIF"
     gene            728..1309
                     /locus_tag="PLI76_02985"
     CDS             728..1309
                     /locus_tag="PLI76_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF99 family protein"
                     /protein_id="HOI61026.1"
                     /translation="MHAAKPGLRALGIAESYSGRKRSTLAGVVMRKDLLIDGVAFARV
                     TVGGSDATDAVVRLVADLARRDINFIMISGCVIAWYNIIDPAAVQEATGLPVIAATYE
                     ESEGLEDDIRHHFPDDPGRLAAYRRLGGRTPIRLHTGYTLFIRPCGLSPGDAARLCND
                     FTHEGRVPEPLRVARLVARGVVRSALCGDVVRP"
     gene            complement(1299..1745)
                     /locus_tag="PLI76_02990"
     CDS             complement(1299..1745)
                     /locus_tag="PLI76_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOI61027.1"
                     /translation="MFKTILVAVDGSDISRRALEEALAVARAMQASLHAVHVVQTGAY
                     PILTSNELEPPDIARQALLDSLEREGDRILAETEGQAAAAGIQITVHKHRGHPGASIT
                     ALAHELGADLTVVGSHGRGRIDRFFLGSVSSYVADHATSTVMIVRA"
     gene            complement(1791..2366)
                     /locus_tag="PLI76_02995"
     CDS             complement(1791..2366)
                     /locus_tag="PLI76_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin"
                     /protein_id="HOI61028.1"
                     /translation="MGCRIRARPPGAVKEAPKLLRGRTLDERSLSHTHAALLMVAITV
                     ILAILLLLILLGMIPSWSWAGPELPPIIITEIVHTNDDTGILTYASRVFLLNNGSTTY
                     ENDCLRAVFYLDGTRVRTVYTLNGYRLIPSHHYGVRYIKGAGCRSPYWNPGEEMEIDL
                     ADKTLIPGGMVTVEIVNRVTGQVISKHTAQA"
     gene            complement(2350..2709)
                     /locus_tag="PLI76_03000"
     CDS             complement(2350..2709)
                     /locus_tag="PLI76_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61029.1"
                     /translation="MSGEHNRARDRLPRRISIVAPAIPGAVAWKKGISSPIRVAGMPG
                     TIDLHQAAHNSPEVEQTVIDYPEAFVTAGRDPSQGILREHADTAGWIGIHDRDSVVDR
                     NTHRQLRNLTGSCGVPY"
     gene            2905..3846
                     /locus_tag="PLI76_03005"
     CDS             2905..3846
                     /locus_tag="PLI76_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOI61030.1"
                     /translation="MVEAQRSRQPGYLRLSGSGELEERAHRAHEVLRDCTICPQECRV
                     NRIEGELGFCRTGLLPMVASYSPHFGEEAPLVGRNGSGTIFFAGCNMRCEFCQNYTIS
                     QCGAGTAISCEDLADIMLHLQERRCHNINLVSPSHVVPQILRAVAIAADRGLSLPLVY
                     NSGGYDSVATLRLLDGVVDIYMPDAKYGRDGVAWELSHARDYTSRMQAALVEMHRQVG
                     DLILSADGIAVRGMIIRHLVLPENLAHSEAVMRFIADEISRDSYVNIMAQYHPAWRAA
                     EGGRSPVLAALQRPITAREYAYAVQCAKESGLARGFS"
     gene            4040..5446
                     /locus_tag="PLI76_03010"
     CDS             4040..5446
                     /locus_tag="PLI76_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HOI61031.1"
                     /translation="MKVRDGTVKRSALTPFVIIAVAVAAVGGILFGYDTGVISGAILF
                     ITSEFALPPTLEEIATSSVLVGAILGAIAGGLLADRIGRRLSIIAASVVFLAGTGIVV
                     GATDLSIFLVGRVLIGIAIGVASFVSPLYISEIAPPAFRGGMVSLNQLFITFGILISY
                     GVDFLFSSSGDWRAMFALGAVPAAVLLIGMVLLPDSPRWLLSRQHMNRAIAVLEKARG
                     TGDVFGELDDIKKAIGMQKGGNWSDLLASSIRMPLIVGLGLAVLQQLTGINTVIYYAP
                     TIFQFSGLHSAGASIAATAGVGTVNVIATAVAVVLIDRVGRRPLLLVGVAGMVVSLAV
                     LGAGFAFSGTVQGGSLLGLITAVSLMTYIASFAIGLGPVFWLLISEIYPLTVRGRAMG
                     VTTIANWAANFLITVTFLTLVGAIGQAVVFWLYALVSIIAWFFVFRLVPETKGLTLEE
                     IEEHFRAGRHPRELKGLP"
     gene            5460..7346
                     /locus_tag="PLI76_03015"
     CDS             5460..7346
                     /locus_tag="PLI76_03015"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HOI61032.1"
                     /translation="MSNQIQQNRPQAHGEYRPIEDYGVIGNGHTVALISSSGSIDWLC
                     FHRFDSPSLFARILDPGRGGYWSVTTKGAFESSHRYQEETNILETTLRCAGGTVVLRD
                     FMDIASIARLRRLPAPGRIVRIAECTDGKVGVVSRCQPRPNYARTHPEFALYGDQVTF
                     GGYTLTGPTAWQVDDADASLTCRITLRAGETTAFTLATEDDATLPQRTPADALKVTTD
                     YWRKWSSACTYRGPYRDIVIRNALTLKMMTYEPSGAIVAAPTTSLPETFGGERNWDYR
                     FTWIRDASLTLYALLLAGYLDNEQPFFDWLVRTVKLKGTGISILYPIIPEGRIAEAVL
                     DNFRGYRDSRPVRIGNQAAVQEQLDVYGEVMGAIQFAWRIGKYDPTPLWGTMRQMLDW
                     VTRHWHDRDSGLWEVRGGVRHFVYSKAMMWFALDCGIEIAEGMRLSGDIKTWRRERDL
                     IHEEVLDKGWSDDLGAFKQSYEDENLDAANLRLPLINFVGGDDPRMLSTIDATLEHLV
                     VDGLCYRYIDAPEGVTGKEGSFVVCTTWLINALIRAGRTGEAHRMFQNLLARASPLGL
                     YAEEIQPATGAHMGNFPQSFSQIGIINAAVSLAHAGQVGTVSPHHAAAADAAGHGGGG
                     KRTG"
     gene            7432..8151
                     /locus_tag="PLI76_03020"
     CDS             7432..8151
                     /locus_tag="PLI76_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOI61033.1"
                     /translation="MKERPGLRYAAASDIGGRQQNEDAFFADEAAGYSVFAVADGLGG
                     HARGDVASKMAIRALEETARECLPGLKPARVLELAFNRANARIYTYNQENRLNAGTTL
                     SAAIVDGSGGCWIGTVGDSRTHVIRSSSVWHTRDQSYVQGLVDAGVLSPVEAMLHPKK
                     NILTGALGLSAGVQVDLDAVDAAGAVLVLSSDGLHDCVPADAIRDIVLADEPDAACRR
                     LIDAAKDAGSTDNITVIVARG"
     gene            complement(8158..8673)
                     /locus_tag="PLI76_03025"
     CDS             complement(8158..8673)
                     /locus_tag="PLI76_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOI61034.1"
                     /translation="MTHAMLPDTGDEHPRDLQPLLIRLFDPDKAVRAEAVHGLIAIGS
                     PAVPACIALLQDADWRVRYRAAEVLGRIGDADAFAPLVAALGDEKDHVRYMAAKGLGL
                     LCDPRAIAHLGVMQRDENEFVRRGAAGSLGRIGGAKAVGLLHAALGDEATEGVRAAIL
                     AALRDAGAEGT"
     gene            complement(8846..8971)
                     /locus_tag="PLI76_03030"
     CDS             complement(8846..8971)
                     /locus_tag="PLI76_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61035.1"
                     /translation="MHVLVKWIESDPVFKTLGPLIVGIGILLLVSLILYALGYLI"
     gene            complement(9186..9258)
                     /locus_tag="PLI76_03035"
     tRNA            complement(9186..9258)
                     /locus_tag="PLI76_03035"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9222..9224),aa:Asp,seq:gtc)
     gene            complement(9275..9381)
                     /locus_tag="PLI76_03040"
     tRNA            complement(join(9275..9310,9344..9381))
                     /locus_tag="PLI76_03040"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9345..9347),aa:Tyr,seq:gta)
     gene            9529..11073
                     /locus_tag="PLI76_03045"
     CDS             9529..11073
                     /locus_tag="PLI76_03045"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOI61036.1"
                     /translation="MKRTVFFTDSRAKKNVTVFDTTLRDGEQTPGISFTREEKFGIAE
                     HLSNIGVHTIEAGFPASSAAEHEIVRAIKGLGLSAQVCGLARSLPADVDACIDCDVDM
                     VHVFIPTSDVQREYTIKKTREQVLAATGDIIAYVRDHIGRCMFSAMDATRTDWDYLME
                     VYRVAVDAGATIINVPDTVGVITPTAMKHLITRIDREVDCPIDVHCHNDFGLAVANTI
                     AAVEGGASQVQVTVNGIGERAGNADLAQTVMILSSIYGVDTGIRTTSLVETSRLVARY
                     AGMSVPAIQPVVGENAFAHESGIHSHGVITRSDTFEPGIMTPEMVGHRRRLKLGKHAG
                     RHAVRQMLAEVHMTPTDAQLDEIILRVKAIAGRGKRVTDADLYEIGESVMQIVSNEKT
                     LELQDIAIMTGNHVIPTASVRATVDGVEHAFSSIGNGPVDAAVRAILGIIPAPVHLKE
                     FNIEAISGGTDALGHVTITVEDERGRVFDASASSDDIILASVEAVINAINLVCRTQKN
                     DKPRKE"
     gene            complement(11098..11463)
                     /locus_tag="PLI76_03050"
     CDS             complement(11098..11463)
                     /locus_tag="PLI76_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit beta"
                     /protein_id="HOI61037.1"
                     /translation="MENIPPKVQNQLAMLQQMQQQLQTVVSQKAQYELTIREARRAVE
                     ELGDVPEDAAVFMSVGGVMMQKGREQVLTSLNERVETLELRVKSLEKQEKALQGRFEQ
                     LSSQIRGALEGKQQPPGAS"
     gene            complement(11735..11971)
                     /locus_tag="PLI76_03055"
     CDS             complement(11735..11971)
                     /locus_tag="PLI76_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Pcc1"
                     /protein_id="HOI61038.1"
                     /translation="MHTAVFTFATDDARALYRSVCQEMGDVGDRSSVRVRLADDNTLL
                     LEATAADIPALRAALNTWLRLINIAIEMHEIVAP"
     gene            complement(11971..12375)
                     /locus_tag="PLI76_03060"
     CDS             complement(11971..12375)
                     /locus_tag="PLI76_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61039.1"
                     /translation="MTVVTTSRKPLPELRSLARDLAFAIGAGYTTRGKAGMGDLFALD
                     ESVVIVSRSGPFFLIQVFVDGETITEITVRSFAVEERPGEIARGLFVSDRPVYEALKD
                     RFNVAFAGETLPRRRIVFDGTQRKRYTLEVLP"
     gene            complement(12372..12503)
                     /locus_tag="PLI76_03065"
     CDS             complement(12372..12503)
                     /locus_tag="PLI76_03065"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit P"
                     /protein_id="HOI61040.1"
                     /translation="MAAYKCARCKQKVEIDVNIRCPYCGYRILFKERGAGIKDLKAR"
     gene            complement(12510..12800)
                     /locus_tag="PLI76_03070"
     CDS             complement(12510..12800)
                     /locus_tag="PLI76_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37ae"
                     /protein_id="HOI61041.1"
                     /translation="MAKRKQSAKGRVVGSSGRFGPRYGRFIRKRINQLEQVSRANHAC
                     PRCDQVAVRREGTGIWACRKCGFKFAGGSYVPETPVMRVAARSIERSLQREG"
     gene            complement(12856..13560)
                     /locus_tag="PLI76_03075"
     CDS             complement(12856..13560)
                     /locus_tag="PLI76_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly factor SBDS"
                     /protein_id="HOI61042.1"
                     /translation="MIPLDQAVVARLESHGERFEVLVDPDQAMRIRRGEEIEIEEAVA
                     ANYIFENAAHAERASDEALLKVFKTTEFEPAALRIIQKGEIHLTSEQRRHLIVEKRNR
                     IITFIARNAVNPQTGFPHPPQRIELAMEEARVNVDPFKSVEEQVKETVKALRPLLPIR
                     FEEIRIAVKIPADYAARAYGELQSAVTLEQNEWQKDGSWIAVVRIPAGAQEELYDLVN
                     RISRGNAETRIIERRS"
     gene            complement(13575..14297)
                     /gene="psmA"
                     /locus_tag="PLI76_03080"
     CDS             complement(13575..14297)
                     /gene="psmA"
                     /locus_tag="PLI76_03080"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit alpha"
                     /protein_id="HOI61043.1"
                     /translation="MQPQYQMGYDRAITVFSPDGRLYQVEYAREAVKRGTTAVGIKCR
                     EGVVLIVDKRVTSRLLEPVSIEKIFKIDTHIGVASSGLVGDARSLVDRARVESQINRV
                     SYSEPINVEILAKKLCDHMQTYTQFGGARPYGTALLIAGVSDGEARLFETDPSGTLLE
                     YKATGIGTGRPAVMKVFEDEYQEDADFSGAIKLGIKALHAATEGKLDVSAIEIGIVSI
                     ATREFKRLDKDEVKVYVDQFEE"
     gene            complement(14297..14779)
                     /locus_tag="PLI76_03085"
     CDS             complement(14297..14779)
                     /locus_tag="PLI76_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpp14/Pop5 family protein"
                     /protein_id="HOI61044.1"
                     /translation="MRPRPPAMRTKRRYILARILPYGATPDQKEVYLSVVEAATSLFG
                     DAAVGLAQPAVVFCDGGYAVIRCRRGTEKDIATALATVTAIGVCRAALRAVAVSGTIH
                     ALKRRVRPIRPLSGDGEMTIEGRCFTMYRYPRQKVDLVEKSIKHQKLLFFTEPDLEER
                     "
     gene            complement(14776..15420)
                     /locus_tag="PLI76_03090"
     CDS             complement(14776..15420)
                     /locus_tag="PLI76_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase P subunit p30 family protein"
                     /protein_id="HOI61045.1"
                     /translation="MKITDASVRPYPAGDSTLARMAVEAAELGFDSVVAIGEGAPPVG
                     GAAVLRGAVISGASPKEVIKQVREPAAQHADVVFVNAGDIAFNRAVVSIKEVQVLRNI
                     HAARKNAFDHVAARSAAQRGLAVDISLAPLIQYRGTKRQRALQQYEDILLLHRRYGFL
                     LTISSGACSILEQRSVREIRGLCALFGMTGAEVNEALASVGRIVEPDQPVRVVG"
     gene            complement(15453..16043)
                     /locus_tag="PLI76_03095"
     CDS             complement(15453..16043)
                     /locus_tag="PLI76_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15e"
                     /protein_id="HOI61046.1"
                     /translation="MAKSMYAYVREAWKRPDRSEVKSLLWERLQQWRREGSIVRVERP
                     TRIDRARSLGYKAKQGIIVVRVKVRRGGRRKPRYIRGRRTARMGMRRMTPAKSLQRMA
                     EERASRKFPNMEALNSYWVGEDGRSKWFEVILVDGHHPSIRSDRNLAWLADPTHRGRA
                     ERGKTSAGMKGRGMRTRGRGTEKTRPSIRSHANRGK"
     gene            complement(16161..16655)
                     /gene="moaC"
                     /locus_tag="PLI76_03100"
     CDS             complement(16161..16655)
                     /gene="moaC"
                     /locus_tag="PLI76_03100"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HOI61047.1"
                     /translation="MNESGRHGRTPVFTHIEDDRAQMVDITAKAEVPREAVASGRIYL
                     REETLRAIREGTTIKGNVLATARVAATLAVKDTPRIIPMCHPIPLGGITIDFAEGDGY
                     IEATARVKSYGRTGVEMEALTGVSVALLTVWDMVKSAEKDENGQYPVTRIDAIRVVEK
                     RKGL"
     gene            complement(16713..18104)
                     /locus_tag="PLI76_03105"
     CDS             complement(16713..18104)
                     /locus_tag="PLI76_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOI61048.1"
                     /translation="MAAGELREFRETGVIDAGRMRAVEENAVALGYPSLLMMESAGRA
                     VADAVLASNPDRILILCGRGNNGGDGMVAARYLQHIGAVDVVYPECGSMTPSAAAQAS
                     LLRHCTAALHPVRCATDVKPLGRLFEEADVIVDAMLGTGASGAVREPLATLVAGANAS
                     PAPIVAVDIPTPGIRANRILSFHRPKVEGADVADIGIPLDAEIYTGPGDLLLVPARSP
                     GAHKGAGGEVLVIGGGPYQGAPYIAAMGALRAGADIVRIASPAYIPMPDLIYERLEGK
                     VITEEHLATILSLVDRTDVVVCGMGLGKESHDVVLAIAEAAERAVFDADALARPLPAA
                     KRTIYTPHAGEFARMTGREPPADLAARARCVRGARTTGTVLLKGPVDVISDGARVRFN
                     RTGTPAMTVGGTGDLLAGIAGALFCRLPAFEAAGIAAYVNGRAGMRAAEGRGCGMLAT
                     DMLDFIPEELFPV"
     gene            18238..19635
                     /locus_tag="PLI76_03110"
     CDS             18238..19635
                     /locus_tag="PLI76_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOI61049.1"
                     /translation="MGLERDVQRAVDRISEADSVTIISHIDADGISTESILAQALARE
                     GIPATSVFVRQLEPMAMRHIPDDDSLKIFTDLGAGQQALLEEHGLSADGVLIVDHHVS
                     QPCGTAYPQVNCLDFGITRMSAAGIAYMVARAIDSANTDLAKLAVVGNVGDMMARENC
                     GLVGPAREIVQDGVEYGNVIVQRADLNCYGISTRPVHVCLGRCDDPYIDGISNSVTRA
                     LRFLERLGVTLKTPRGGWLVWEEVPLDDRRTIISALTQQLVAHGREADRLLAETYIFP
                     DEPERTPFRNASEYATLLNACGRWAKPKVGGRICRGDRGDAYREAEYMLAHHRSVIRG
                     LLQYILETGVTELSHLQYIHTGDRFPDTIVGIGAGMALSKLNWKKPIMVLAAMVDEPD
                     VTKVSMRTNEWALARGVDLQAALVEASATVGGAGGGHRIAAGAFIPHDTEEEFIDGIN
                     RILTRQFASADTDNS"
     gene            19577..20077
                     /locus_tag="PLI76_03115"
     CDS             19577..20077
                     /locus_tag="PLI76_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOI61050.1"
                     /translation="MVSTEYSRDSSLRRIRTIADFQFGAGSGEALFPEGCTFEFSTTG
                     RIRQVRLGNERLVTVRAQDGRLTLGIAGAARLAAVLAPPTYRVTVREDVAAFIADGKN
                     AMAKHVVAADEGIRAGDEVLVVTGDDMLLATGAALLSGREILAFNYGVAVKVRQGRGS
                     ECFQAR"
     gene            20059..20400
                     /locus_tag="PLI76_03120"
     CDS             20059..20400
                     /locus_tag="PLI76_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nascent polypeptide-associated complex protein"
                     /protein_id="HOI61051.1"
                     /translation="MFPGKINPRKMKQMMKQMGMEMEELEGVEKVVIYTPAGNYVFDD
                     AQVVATTMQGVTTYQLTGEARFEEAVPEIPDEDVDLVASQAGVAGEVAKKALIETRGD
                     IAEAILKLTQQ"
BASE COUNT         3690 a         6402 c         6472 g         3909 t
ORIGIN      
        1 aaaatatggg gttgttcatc ttcgcaagcc ggtatatggt cgagaagatc ctcgcgccgt
       61 cctggagggg atttaacttg gtggcggtgc cgggccgggc gaggtaccgg accgggacga
      121 ccgtgatccg ctgcccgtgc ctcaccgcct cgaccgccat ctccgtctcg atctcgaacc
      181 ccgactcttt gagggtcatc tgccggatgg aggggagggt aaaggcccgg tagccggaga
      241 ggatgtcgtg gaggtctctc ccgtgtgcga gcttgaacat gaggttgagt atctggttgc
      301 caaggaggtt taaccgggtg aacgcctccc cctcggggcc ggcgagacgg tccccgatga
      361 cgtggtcgaa cccgaggccg agcgggtcga gcatccgttc cgcatcatcc ggcgcgtagg
      421 tgccgtcgcc gtcgagcatg agcacgtagg gcttatcgat gatcgccacg gcctcgatta
      481 tggcgttgcc cttccccctg cccgtctgcg tccgaacgac cgccccggca gctcgggcga
      541 tatcaggcgt cccatcggtg ctgtgcccgt ccatgacgag gatgtggcga aaaccccgtt
      601 ccttaaactc cccgatcagc tcggcaatcg tcggggcctc atttaatgtg ggaataaaga
      661 tacagacctc gtctcgctcg atgttcatcg gtgtactata tccgattgag caacataaga
      721 acttcgcatg cacgcagcca aaccggggct tcgtgctctc ggtatcgccg agagctactc
      781 gggtcggaag cgatccaccc ttgccggcgt cgtgatgcgt aaagacctcc tcatcgacgg
      841 agtagccttc gcccgggtga ccgtcggggg gtcggatgcg accgatgcgg tcgtccgtct
      901 tgtcgccgat ctcgcacgcc gggacatcaa cttcatcatg atcagcggtt gcgtcatcgc
      961 ctggtacaac atcatcgatc cggcggcggt gcaggaggcg accggcttac ccgtcatcgc
     1021 cgccacttac gaggagtcgg aggggctcga ggatgatatc cgccaccact tcccggacga
     1081 tcccggccga ctcgctgcat accggcggct cggtggccgg acgcctatcc ggctccatac
     1141 cgggtacacg ctcttcatcc gcccctgcgg cctctctccc ggggatgcag cacggctctg
     1201 caacgacttc acgcacgaag gacgggtgcc cgaaccgctc agggtggcgc ggctcgtcgc
     1261 ccgcggcgtc gtccggtcgg ccctctgcgg tgatgttgtc aggccctgac gatcatgacc
     1321 gtcgacgtcg cgtggtcggc gacgtaggaa ctgacgctcc cgaggaagaa gcgatcgatc
     1381 cggccgcggc cgtgcgatcc gacaacggtc aggtcggcgc cgagttcgtg cgccagtgca
     1441 gttatactgg ccccgggatg gcccctgtgc ttatggacgg ttatctggat gccggcagcg
     1501 gccgcctgcc cttccgtctc ggcaaggatc ctgtctccct cccgttcgag cgaatcgagg
     1561 agcgcctgcc gcgcgatatc gggaggctcg agctcgttcg aggtcagtat ggggtacgct
     1621 cccgtctgga cgacgtgcac ggcgtggagt gatgcctgca tcgcacgggc gactgccagc
     1681 gcctcctcga gtgcacgacg gctgatatcc gacccatcga ccgccacgag aatcgtcttg
     1741 aacatacctc tcttttagga gcgtgccagt acaaatagat gtctctgcgg tcatgcctgg
     1801 gcggtgtgct tcgatatcac ctgccccgtc actctattca cgatctccac ggtgaccatc
     1861 ccgcccggga tgagggtttt atcggcaaga tcgatctcca tctcctcgcc gggattccag
     1921 tatggggagc ggcaccccgc acctttgatg tagcgcaccc cataatggtg cgatggtatg
     1981 aggcggtaac cgttcagggt atagacggtg cggactctcg tgccgtccag gtaaaaaacc
     2041 gccctcaggc aatcgttctc gtaggtcgtg ctgccgttgt tgaggaggaa taccctgctt
     2101 gcataggtca gtattccggt atcatcgttt gtgtggacga tctcagtgat aatgatcggc
     2161 ggcagttcag gccccgccca cgaccacgag gggatcatcc cgagcaggat caggaggagg
     2221 aggatggcaa ggatgaccgt gatggcgacc atcaggagcg cggcgtgggt gtgcgagagg
     2281 ctgcgctcat ccagggtgcg ccctctcagg agtttcggtg cctcctttac ggcacctggc
     2341 ggccgggccc taatacggca ccccacacga cccggtcagg ttccggagct gcctgtgggt
     2401 gttccggtct accacgctat cccgatcatg gatcccgatc cacccggcgg tgtcggcatg
     2461 ctcccgcaag atcccttgag aaggatccct gcccgctgtg acgaaggcct ccgggtaatc
     2521 gatgaccgtt tgctctactt ccgggctatt gtgggcagcc tgatggagat cgatcgtacc
     2581 cggcatcccg gcgaccctga tcggagaaga gatccctttc ttccaggcga ctgcaccggg
     2641 gatggcggga gcgacgatcg agattctgcg cggcaggcgg tcgcgcgcac ggttgtgttc
     2701 gccggacata cggtaatgga gaatcggtca cgcaggatat aagtattgtc aaaatagtcc
     2761 ttttcctggc cgggaaatca ggaaataata ttcgaaacgg gccgtggttc cgggccggcg
     2821 ccggagtgta ctcttcgccc gccgcagggc gaggcctgca gacgcagggt aaaccttctt
     2881 ttgtctcacg caaaggagga gatcatggtt gaagcacaac gatcccggca gcccgggtac
     2941 ctgcgcctat ccgggagcgg cgaactggag gagcgggcgc accgggcgca cgaggtgctc
     3001 cgcgactgca ccatctgtcc ccaggagtgc cgcgtgaacc gtatcgaagg cgaattgggg
     3061 ttctgccgga ccggcctcct cccgatggtt gcaagttaca gcccgcactt cggcgaggag
     3121 gcaccgctcg tggggaggaa cgggtcagga acgatattct ttgcgggctg caacatgcgc
     3181 tgtgagttct gccagaacta cacgatcagc cagtgcggcg ccggcacggc gatatcgtgc
     3241 gaagatctcg cggacatcat gctccacctg caggaacgcc ggtgccacaa catcaacctc
     3301 gtctccccct cgcacgtcgt cccccagatc ctccgtgcag tcgccatcgc tgccgatcgc
     3361 ggcctctccc ttccgctggt ctacaacagc gggggatacg actctgttgc aactctgcgg
     3421 cttcttgacg gcgtcgtcga catctacatg ccggacgcga agtacgggcg cgacggcgtg
     3481 gcgtgggaac tctcccatgc tcgcgactac acctcccgca tgcaggccgc gctcgtggag
     3541 atgcaccggc aggtcggaga cctgattctt agcgccgatg gcattgcagt ccggggcatg
     3601 atcatccgtc acctggtgct ccccgagaac cttgcacaca gcgaggccgt gatgaggttc
     3661 atcgccgatg agatctcgcg ggactcttac gtgaacatca tggcccagta ccacccggcg
     3721 tggcgggcgg ccgaaggggg aaggagcccg gtgcttgcgg cgctgcagcg cccgatcacg
     3781 gcgcgggagt atgcgtacgc cgtccagtgc gcaaaggaga gcggccttgc ccgcgggttc
     3841 tcctaggagc cccggaagat ccccggcacc agttcccggc gaaactgccg ggcgcggttc
     3901 catcgctatg ttttatatcg atgcaggtga agcaccccgt cgcctcacga tacgttgccg
     3961 ggcacatcat cccggataac ctgttccgga ttagcccatc ccggagcagg ttgtttacgc
     4021 attgagcggg gagcgaaaga tgaaagtgcg ggatggaacg gtgaagagat cggccctcac
     4081 gccattcgtc atcattgccg ttgcagtggc ggccgtcgga gggatcctct ttggctacga
     4141 tacgggagtc atatccgggg caatcctctt cataaccagc gagtttgctc tccccccgac
     4201 cctggaagag atcgcgacca gctcggtgct cgtcggcgca atcctgggtg cgatcgccgg
     4261 cggcctcctt gccgatagga tcgggcggcg cctctcgatc atcgctgcgt cggtcgtctt
     4321 ccttgcaggc accgggatcg tcgtcggtgc aacggacctc tccatcttcc tggtcggccg
     4381 cgttctgatc ggcatagcca tcggtgtcgc atcgttcgtc agccctctct acatatcgga
     4441 gattgcgcct ccggcgttcc gcggcggcat ggtgtcgctc aaccagctct tcatcacgtt
     4501 cgggatcctc atctcctacg gggtcgactt cctcttctct tcaagcggcg actggcgggc
     4561 catgtttgcc ctcggcgcgg tgccggccgc ggttctcctc atcggcatgg ttctgctccc
     4621 ggatagcccc aggtggcttt tgtcccgaca gcatatgaac cgggcaatcg ccgtcctcga
     4681 aaaagcccgc gggaccggtg atgtctttgg ggagcttgat gatatcaaga aggcgatcgg
     4741 gatgcagaag ggcggcaact ggtcggacct gctggcttca tcgatacgaa tgcccctgat
     4801 cgtcggtctc ggcctggccg tcctgcagca gctgaccggc atcaacaccg tcatctatta
     4861 cgccccgaca atctttcagt tttcagggtt gcactctgcc ggcgcttcga ttgcagcgac
     4921 tgcgggcgtg ggcaccgtga acgtcattgc caccgccgtt gccgtcgtgc tcatcgatcg
     4981 ggttggacgc cgccctcttc tcctcgtggg ggtcgccggc atggtcgtga gccttgccgt
     5041 gctcggtgcc gggtttgctt tcagcggaac ggtgcagggc gggagtctcc tcgggctgat
     5101 caccgcggtc agcctgatga cgtacatcgc ttcgtttgct atcggcctcg ggccggtgtt
     5161 ctggctcctg atatccgaga tctacccgct caccgtgcgg gggcgtgcca tgggcgtcac
     5221 aaccatcgcc aactgggccg cgaacttcct catcaccgtg acattcctga cgctggtcgg
     5281 agcaatagga caggccgtcg tcttctggct ttatgcgctc gtgagcatca tcgcgtggtt
     5341 cttcgtcttc aggctggtgc ccgagacgaa aggactcacg ctcgaagaga ttgaagaaca
     5401 cttcagggcc ggcaggcacc cccgcgagct gaagggttta ccgtgatcac gtgatcgata
     5461 tgtccaacca gatccagcag aaccggccgc aggcccatgg tgagtaccgg ccgatcgagg
     5521 actacggtgt gatcgggaac gggcacaccg tggcgctcat cagcagcagt gggtccatcg
     5581 actggctctg ctttcacagg ttcgactctc catcgctctt tgcccggata ctcgatcccg
     5641 gccgcggcgg atactggagc gtgacgacga aaggagcgtt cgagagctcg caccggtatc
     5701 aggaggagac aaacatcctc gagacgaccc tcaggtgtgc cgggggaacc gtggtcctcc
     5761 gggacttcat ggatatcgca agcattgccc gcctccgccg gctcccggcc cccggcagga
     5821 tcgtacgcat agcggaatgc accgatggga aggtgggggt cgtctcccgc tgccagcccc
     5881 ggccgaacta cgcccgcaca caccctgagt ttgcgcttta tggagatcag gtaacgttcg
     5941 gtgggtatac gctcaccggg ccgacggcat ggcaggtcga cgacgcagat gcatcgttga
     6001 cctgcaggat cacgctccgt gcgggcgaga cgacggcgtt cacgctcgca acggaggacg
     6061 acgccaccct gccgcagcgt acgcctgcgg atgccctgaa ggtgacgacc gattactggc
     6121 gaaaatggag cagcgcctgc acgtaccggg ggccgtatcg cgatatcgtc atccgcaacg
     6181 ccctcactct caagatgatg acctacgagc cgtccggggc catcgttgcc gcgccgacga
     6241 catcgctccc cgagaccttc ggcggcgagc ggaactggga ttatcgcttc acctggatac
     6301 gcgatgcgtc gctcacgctg tatgccctcc tccttgccgg ctacctcgac aacgagcagc
     6361 cgttcttcga ctggctcgtg cgcacggtca aactgaaggg gacgggcata tcgatcctct
     6421 atccgatcat accggagggc aggatcgccg aggcggtgct cgataacttc aggggatacc
     6481 gggattcccg gccggtgagg attggaaacc aggcagcggt ccaggagcag ctcgacgtct
     6541 acggcgaggt catgggggcc atccagttcg cctggcggat cgggaagtac gacccgacgc
     6601 cgctctgggg gacgatgcgg cagatgctcg actgggtgac ccgtcactgg catgaccgcg
     6661 atagcggact ctgggaggtc aggggcggcg tgcggcattt tgtctacagt aaagccatga
     6721 tgtggttcgc tcttgactgc gggatagaga tcgcggaggg tatgcgcctt tctggggata
     6781 tcaagacctg gcgccgcgag cgcgacctga tccacgagga agtgctcgat aaggggtgga
     6841 gcgatgatct gggtgcgttc aagcagtcgt acgaggatga gaacctcgat gccgcgaacc
     6901 tgcgcctccc cctcatcaac ttcgtcgggg gggatgatcc gcggatgctc tcgacgatag
     6961 atgcgacgct cgagcacctt gtcgtcgacg gactctgcta ccgctacatc gatgctcctg
     7021 agggcgtgac cgggaaggaa gggtcgttcg tcgtctgcac gacctggctc atcaacgcgc
     7081 tgatacgtgc cgggcgcacc ggagaggcgc accggatgtt tcagaacctt cttgcacgtg
     7141 caagtcctct cggcctctac gccgaagaga tccagccggc gaccggggcc catatgggca
     7201 acttcccgca gtcgttctca cagatcggta tcatcaacgc cgccgtctcg cttgctcacg
     7261 cagggcaggt cggcacggtc agcccgcacc atgccgccgc tgcggatgcg gcgggacacg
     7321 gcggcggggg caaacgcacc gggtgaggcg aagggtgacg acggtgaagg gagatggctc
     7381 cggggaccgg gcaccaaaag gctattcgtc cggccatcca gagtggtata cgtgaaggag
     7441 aggcctgggc tccggtacgc agcggcgagc gatatcggcg ggcggcagca gaacgaggac
     7501 gcgttctttg ccgatgaagc ggccggatac tccgtcttcg ccgtcgcgga cgggctcggg
     7561 gggcatgccc gcggggatgt tgcgagcaag atggcgatcc gggccctgga ggagaccgcg
     7621 agggagtgcc ttcctggatt aaaacccgcc cgggtgcttg aactggcatt caatcgggca
     7681 aacgcccgga tatatacgta taaccaggag aaccgcctga acgccgggac gaccctctcg
     7741 gccgcgatcg tcgatggctc cggggggtgc tggatcggca ccgtgggcga cagcaggacg
     7801 cacgtcatcc gatcatcgtc cgtctggcat acgcgcgacc agagttacgt ccagggcctg
     7861 gtggatgcag gggtcctctc ccccgtcgaa gcgatgcttc atccgaagaa gaacatactg
     7921 accggggcgc tcggcctctc ggccggggtg caggtcgacc ttgatgccgt ggatgccgcc
     7981 ggggcggtcc tggtgctcag ctcggacggg ctccatgact gcgttccggc ggatgcgatc
     8041 cgcgatatcg tgctcgccga cgagcctgat gcggcatgcc ggaggctgat cgatgccgcg
     8101 aaggatgcgg gaagcaccga caacataacg gtgatcgtcg cgagagggtg acggggctca
     8161 ggtgccctct gctcccgcat cgcggagcgc cgcaaggatt gccgcacgga cgccttcggt
     8221 cgcctcatcc ccgagtgctg catggagcag gccaacagct ttcgcgccgc caatcctgcc
     8281 gagggaaccg gcagcgcccc gtcgcacgaa ctcgttctcg tcgcgctgca tcaccccgag
     8341 gtgtgcaatc gcccggggat cgcagagcag cccgagcccc tttgccgcca tgtagcggac
     8401 gtggtctttc tcatcgccga gcgcggcaac gaggggtgcg aatgcatcgg cgtcgccaat
     8461 ccggccgagg acctccgccg cacggtagcg cacccgccag tcggcatcct gcagcagggc
     8521 gatgcaggca ggaacggcag ggcttccaat cgctataagt ccgtgcacgg cttctgcccg
     8581 cacagccttg tccgggtcaa agagtcggat aaggagaggt tgcaggtcgc gcgggtgttc
     8641 atctccggta tccggcagca tggcgtgggt catatccagt cacatctgcg ggttgtagcc
     8701 gcttgcatca catgttgagg cggcgttgtg ataagagttg atcatttgcc tccggccggt
     8761 gccggggata ccgccgcacg agagggtatc tggaggcgcc gggaaccacc atacggtgca
     8821 gtatcccgac tcccccggtg tgccgtcaga tcagatatcc cagcgcatat agtatcaggc
     8881 ttacaagaag caggatgccg atcccgacga tgagcggccc cagagtcttg aatacgggat
     8941 cgctctcaat ccatttcacc agaacgtgca tggcaccgga tacaccattc ggatatatat
     9001 attttatctt aaaaataaaa atcacatctg atatttaatg gtagtgcatc gcgccaggta
     9061 cagtgccggg gaggttaacg gttatccggc aactttcggc agttctcggt ctattccggg
     9121 tggcgagaag acttctcttg ccggggatgc gctctgctcg gaccttgaga aaatgggaga
     9181 aatgacgccc aggacggaat tcgaatccgt gtcgacggcg tgacaggccg tcatgatagg
     9241 ccactacact acctgggcac ttttgatggg tggatcccgc ctcccggatt cgaaccgggg
     9301 acatcgcggt agccgcgcag ttcgcccgag agcgaagact atactacagc cgcgcactct
     9361 accagtctga gttaaggcgg gtttctgctc taacaatgtt gtcgtgggtt gttattaaat
     9421 atttcgtaga atccccggga tgttgcacga tccggagctc tggctatata tacaacctct
     9481 gataatgagt aagtatgaac cctacaaacg ctgaagcacc caggaacgct gaagcgtact
     9541 gtcttcttca ccgatagccg tgcgaaaaag aacgtcactg ttttcgacac cacactgcgc
     9601 gacggtgaac aaacgccggg catctcattc acgcgtgaag agaagttcgg tattgcagag
     9661 catctctcga acataggagt ccacaccatc gaagcgggct ttcccgcatc ttctgctgcc
     9721 gagcacgaga tcgtcagggc catcaagggc ctcggtctct ccgcacaggt atgcggcctc
     9781 gcgcggtcgc tcccggccga cgtcgatgcg tgcatcgact gcgacgtcga tatggtccac
     9841 gtcttcatac cgacatccga cgtccagcgg gagtacacca ttaaaaagac tcgcgagcag
     9901 gtcctcgcgg ccaccggcga tatcatcgcg tacgtccgcg accacatcgg ccggtgtatg
     9961 ttctccgcca tggatgccac caggaccgac tgggattacc tgatggaggt ctaccgcgtc
    10021 gcggtggatg cgggagcgac gatcatcaac gtccccgata ccgtcggcgt gatcaccccg
    10081 acggccatga agcacctcat cacccggatc gaccgggagg tcgactgtcc gatcgacgtc
    10141 cactgccaca acgacttcgg gcttgcggtg gcaaacacca tcgcggcggt cgagggcggc
    10201 gcctctcagg tgcaggtgac ggtgaacggc atcggcgagc gggcgggaaa cgccgatctc
    10261 gcgcagacgg tgatgattct ctcgtctatt tacggtgtag ataccggcat ccggacgaca
    10321 agcctggtcg agacctcgcg gctcgtcgca cgctacgccg gaatgagcgt ccccgccata
    10381 caaccggtcg tcggcgagaa cgcctttgcc cacgagagcg gtatccactc ccacggcgtg
    10441 atcacgcggt cggatacgtt cgagcccggt atcatgacgc cggagatggt cggccaccgc
    10501 cgcaggctga aactcggcaa acacgccggc aggcatgcgg tccggcagat gctcgccgag
    10561 gtgcacatga cgcccaccga cgcgcaactc gacgagatca tcctgcgggt gaaggcgatt
    10621 gccggcaggg gcaagcgggt gacggatgcg gatctctatg agataggaga gagcgtcatg
    10681 cagatcgtgt ccaacgaaaa gacactcgag ctccaggata tcgccatcat gaccggcaac
    10741 cacgtcatac cgacggcgag cgtcagggcg accgtcgatg gggttgagca cgccttctca
    10801 agcatcggca acgggccggt ggatgcggcc gtcagggcaa tccttggcat catccctgcc
    10861 ccggttcacc taaaagagtt caacatcgag gctatatccg gtggcaccga tgccctcggg
    10921 cacgtcacca tcaccgtcga ggacgagcgg ggccgggtgt tcgatgccag cgcctcaagc
    10981 gacgacatca tcctcgcatc ggtcgaggcg gtgatcaacg cgatcaacct cgtctgccgg
    11041 acgcagaaga acgataaacc gcggaaagag tgagttctcg ataaaaaaaa ttcaggttca
    11101 ggaagcgccg ggaggctgct gtttgccttc cagcgctccc cggatctgcg aggagagctg
    11161 ctcgaacctg ccctgcaggg ccttctcctg cttctcaagc gacttgaccc ggagttcgag
    11221 cgtctcgacc cgctcgttta aggaggtgag cacctgctcc ctgcccttct gcatcatgac
    11281 gccgccgacg ctcatgaaga ccgccgcatc ctcggggaca tcgccgagct cctcgaccgc
    11341 tcgcctggct tcgcggatag tcagttcata ctgcgctttc tgtgagacca cagtctgcag
    11401 ctgctgctgc atctgctgga gcatagccag ctggttctgg actttcgggg ggatattctc
    11461 catattcatc aatcctcctt cgaatgggat gggagtagca tgatctcggg ggcaggggcc
    11521 tttgcggccc tctgtccatt ctctacatga tagggcgggg aacccttaaa aggtgagggt
    11581 tcggaagaga gaatattgtc ccgtaacgag ccgcatggta cgctcgttcg accgcccagg
    11641 ggaggttgac ggttcggaag agagaatgtt gtcctgtaac gggccgcggg cggtctatcc
    11701 cgggaggggg tgccggcgac ccgttccggc atcgtcaggg ggcgacgatc tcgtgcatct
    11761 cgatcgctat attgatcagt cgcagccagg tattgagggc cgcacggagc gccggtatat
    11821 cggcggcagt cgcctcgagg aggagcgtat tgtcgtccgc gagccgtacg cggacggacg
    11881 accggtcccc cacgtcgccc atctcctggc agacggagcg gtagagggca cgcgcatcgt
    11941 cggtcgcaaa cgtaaagacc gccgtatgca tcacgggaga acctccaggg tgtagcgctt
    12001 ccgttgcgtc ccgtcaaaga cgatccggcg ccggggaaga gtttcgccgg caaacgcgac
    12061 gttgaagcga tccttgagcg cctcgtatac ggggcggtcg gagacgaaaa gcccgcgggc
    12121 gatctctccg ggccgctcct cgacggcaaa cgaccggaca gtgatctccg ttatcgtctc
    12181 accatccaca aatacctgaa tgagaaaaaa aggacctgat cgtgagacga tcacgaccga
    12241 ctcatcgaga gcgaagaggt cgcccatgcc cgccttgcca cgggtggtat acccggcgcc
    12301 gatcgcaaac gccagatccc gggcgaggga gcgcaactca ggaagcggtt tacgtgacgt
    12361 cgtgacgacc gtcatcgggc cttcagatct tttatgccgg ccccgcgctc tttgaagagg
    12421 atacggtatc cacagtaagg acagcgtata ttgacgtcga tctccacttt ctgcttgcag
    12481 cgagcgcact tataggcggc cacgaccggt tagccctccc tctgcagcga gcgctcgatg
    12541 ctccgcgcgg cgacgcgcat caccggcgtc tcggggacgt aacttccgcc cgcaaactta
    12601 aacccacact tgcggcatgc ccagatcccg gtaccctcgc gcctgactgc tacctgatcg
    12661 cagcgggggc acgcgtggtt cgcgcgggag acctgctcga gctggtttat tctcttccgg
    12721 ataaaccggc cataccgcgg accaaaccgt cccgaacttc cgactaccct gccctttgca
    12781 ctctgtttac gctttgccat ttcgcgtacc tcttatatgt cctctaattt ttgtgctgtc
    12841 gattaatata gttactcacg acctgcgctc gatgatccgg gtctcggcat tcccccggga
    12901 tatcctgttg accaggtcgt agagttcttc ctgggcaccc gccggaattc tgacgacggc
    12961 gatccaggaa ccgtccttct gccactcgtt ctgttcgagc gtcaccgcgc tctggagctc
    13021 gccgtaggcc cgcgcggcat aatccgccgg gatcttcacg gcgatgcgga tctcctcaaa
    13081 ccggatgggg aggaggggcc tgagcgcttt gaccgtctct ttgacctgct cctcgacgga
    13141 cttgaaggga tcgacgttca cccgtgcctc ctccatggca agctcgatcc gctgcggagg
    13201 atgggggaag ccggtctggg ggttgaccgc gttcctcgca atgaacgtga tgatccggtt
    13261 ccgcttctcg acgatgagat ggcggcgctg ctccgacgtg aggtggatct cgcctttctg
    13321 gatgatccgg agtgccgccg gttcgaactc ggtcgtcttg aagactttca ggagggcctc
    13381 gtcggaggcc cgttcggcat gggcggcatt ctcgaagata tagttggccg cgacggcctc
    13441 ctcgatctcg atctcttccc cccgccggat gcgcatggcc tggtcggggt cgacaagcac
    13501 ttcaaaccgc tctccgtggc tctcaagccg cgctacaact gcctggtcca ggggtatcat
    13561 aggaggtgcc cgctctactc ttcgaactga tcgacgtaga ccttcacttc atctttatcg
    13621 agtcgcttga actcccgggt cgcgatggag acgataccga tctcgatggc gctcacatcc
    13681 agtttgcctt ccgtcgcggc gtgcagggcc ttgatgccga gcttgattgc gccggaaaag
    13741 tcggcgtcct cctggtactc gtcctcaaag accttcatga ccgcaggccg gcccgtgcct
    13801 atcccggttg ccttgtactc gagcagcgtg ccgctcggat cggtctcaaa gagccgggcc
    13861 tccccgtcgc tgaccccggc aatcaggagg gctgtcccgt aggggcgtgc accgccgaac
    13921 tgggtgtagg tctgcatatg gtcgcagagc ttctttgcca ggatctcgac gttgatcggc
    13981 tcgctgtagg agacacggtt gatctgcgac tcgacccgcg ctctgtccac gagagaccgc
    14041 gcatcgccga cgaggccgga ggatgccacg ccgatatgcg tgtcgatctt gaagatcttc
    14101 tcgatggaga ccggctcgag gaggcgggac gtcacccttt tatccacgat cagcacgact
    14161 ccttcgcggc acttgatgcc gacggccgtc gttccccgtt tcactgcttc tcttgcgtac
    14221 tcaacctggt agagccttcc gtccgggctg aataccgtga tcgcccggtc atatcccatc
    14281 tgatattgtg gttgcattat cgttcctcca aatcgggttc ggtgaaaaac aatagtttct
    14341 gatgcttaat acttttttca accaaatcaa ccttttgacg tggatagcgg tacatggtaa
    14401 aacatctccc ttcaatcgtc atctccccgt ccccggaaag ggggcggatc ggtctcaccc
    14461 ggcgcttgag ggcgtgaatg gtcccggaga ccgcgaccgc ccggagggcc gcccggcaaa
    14521 cgccgatcgc ggtgaccgtc gcaagggcgg tggcgatatc cttctccgtt ccccgccggc
    14581 accggataac ggcgtatcct ccgtcgcaga agaccaccgc cggctgtgcg aggccgacgg
    14641 ctgcatcgcc aaagagcgat gtcgcggcct cgacgaccga gaggtagacc tccttctggt
    14701 ccggggtcgc cccatagggg aggatccgcg ccaggatgta gcgccgcttt gtccgcatcg
    14761 ccggcggtct tggtctcatc ctaccacccg cacgggctga tcgggttcga cgatgcggcc
    14821 gacggatgcg agagcctcgt tcacttccgc tccggtcatg ccgaagagcg cgcagaggcc
    14881 ccggatctcg cgaaccgacc gctgttcgag gatcgagcat gcgcctgagg atatcgtcag
    14941 caaaaagccg tagcgccggt gcagcaggag gatatcctcg tactgctgca gcgccctctg
    15001 gcgcttcgtc ccccggtact ggatgagggg ggccagcgat atatccacag caagaccgcg
    15061 ctgtgccgcc gaccgtgcgg cgacatggtc gaaggcgttc ttccgggcgg cgtggatgtt
    15121 cctgagaacc tgcacctctt tgatcgagac gacggcacgg ttgaaggcga tatctcctgc
    15181 attgacaaag accacgtcgg cgtgctgggc ggcaggctcg cgcacctgct tgatgacctc
    15241 ttttggggat gcgccgctga tcacggcacc gcggagaacc gccgcccccc cgaccggggg
    15301 agcaccctcg ccgatggcga cgacgctgtc aaacccgagt tccgctgcct cgaccgccat
    15361 ccgcgcaagc gtcgagtcgc cggcgggata gggacgtacg ctcgcatctg tgatcttcat
    15421 cgtcgagagg ccgtatgtaa aaagagggtt atttatttac cgcggttcgc gtgggagcgg
    15481 atgctcgggc gggtcttctc ggtcccgcgc ccgcgcgtcc gcatgccgcg gcccttcatg
    15541 ccggcggagg tcttgccgcg ctccgcccgg ccccggtgag tcgggtcggc cagccacgca
    15601 aggttgcggt cgctccggat cgatgggtgg tgaccgtcga cgaggatgac ctcgaaccat
    15661 ttggaccgtc cgtcttcccc gacccagtag gagttcaggg cctccatgtt cgggaacttg
    15721 cgggatgcgc gttcctcggc catacgctgc aggctctttg ccggggtcat ccggcgcatg
    15781 cccatgcgtg ctgtcctgcg cccgcggatg taccggggtt tcctccggcc gccgcggcgg
    15841 accttgaccc gcacgacgat gataccctgt ttggccttgt atccgagcga tcgggcccgg
    15901 tcgatacggg ttgggcgctc cacgcgcacg atgctcccct cgcggcgcca ctgctggagg
    15961 cgctcccaga ggagactctt tacttccgac ctgtccggcc gtttccatgc ctcgcgaacg
    16021 taggcataca tcgattttgc cattctcaga tcacctcagg ttcagcgttt ccgccacatt
    16081 cctttccggg acgtatcccg tggttcctaa caggttgacg ggagcgctaa taaagaattc
    16141 tcttctgccg gctcccgcgc ctacaacccc ttgcgcttct ccacgacgcg gatggcatcg
    16201 atccgggtga cggggtactg cccgttctcg tctttttccg ccgatttgac catatcccag
    16261 acggtgagga gagcgaccga gacaccggtg agggcctcca tctcgacgcc cgttcttccg
    16321 taggacttca cgcgggcggt cgcctcgatg tagccgtcgc cctctgcaaa gtcgatcgtg
    16381 atgcccccga gaggtatggg gtggcacatg gggatgatgc gcggcgtatc cttcaccgcg
    16441 agggtggccg ccacccgtgc ggtcgcgagc acgttgcctt tgatcgttgt tccttcgcgg
    16501 atggcccgga gcgtctcttc ccggaggtag atcctgccgc ttgcaaccgc ttcccggggg
    16561 acctcggcct tcgccgtgat gtccaccatc tgcgcgcggt cgtcctcgat atgggtgaag
    16621 accggggtcc tgccgtgtct gccggactcg ttcatgggta tcgcaggaga tcgtctgtct
    16681 cttgagggat aaaagagggc gtctccagga aattataccg ggaagagctc ctccggtatg
    16741 aagtcgagca tatcggtggc gagcataccg catccccgcc cctctgcggc acgcatgccg
    16801 gctcttccgt tcacgtaggc ggcgatgccg gccgcctcga aggccgggag gcggcagaag
    16861 agcgctcccg caatcccggc gagcaggtcg cccgtccccc cgacggtcat cgccggcgtc
    16921 ccggtccggt tgaacctgac ccgcgccccg tcggatatga catcgaccgg gcctttgagg
    16981 aggacggtcc ccgtcgttct cgcgcccctc acgcaccggg cgcgtgcggc gaggtccgcc
    17041 gggggttccc tcccggtcat ccgggcgaac tcgcccgcgt gcggggtgta tatcgtcctc
    17101 tttgcggccg gcagggggcg ggcgagcgca tcggcatcaa agaccgcccg ctctgccgcc
    17161 tctgcgatgg cgagcacgac gtcgtggctc tctttcccga ggcccatgcc gcagacgacg
    17221 acgtcggtgc ggtcgacgag ggagaggatc gtcgcgaggt gctcttcggt gattaccttc
    17281 ccttccaggc gctcgtagat aaggtcgggc atggggatat aggccgggga ggcgatgcgc
    17341 acgatgtcgg ccccggcacg gagcgccccc atcgccgcga tgtagggcgc tccctggtag
    17401 ggtccgcccc cgatgacgag aacctcgccg ccggcgccct tgtgcgctcc gggcgagcgc
    17461 gccgggacga gcaggaggtc gccggggccg gtgtaaatct ctgcatcaag cgggatgccg
    17521 atatccgcga cgtccgcgcc ctcgactttt ggccggtgga acgagaggat ccggttcgcc
    17581 cggatgcccg gggtggggat atcgacggcg acgatcggtg ccgggctcgc gtttgccccg
    17641 gcaacgaggg tcgcgagcgg ctcacggacc gcgcccgatg ccccggtccc gagcatggcg
    17701 tcgacgatga catccgcttc ctcaaagagg cggccgaggg gtttgacgtc ggtcgcgcac
    17761 ctgaccgggt ggagggcggc cgtgcagtgc cggaggaggg atgcctgggc cgcggccgaa
    17821 ggggtcatcg aaccgcattc cgggtagacg acgtcgaccg caccgatatg ctggaggtac
    17881 cgggccgcaa ccatcccgtc gccgccgttg tttcccctcc cgcagaggat gaggatacga
    17941 tcggggttgc tcgcgaggac ggcatccgcg accgcccggc cggcgctctc catcatgagg
    18001 agcgacgggt agccgagcgc aacggcgttc tcctcgaccg cccgcatccg gcccgcatcg
    18061 atcacgccgg tctcccgaaa ctcccgcaat tcgcctgctg ccatgtatcg ttcctccgga
    18121 gcgttgcctg atgggtgcta tgatagttct ctcttagata ccttttgcgt gggattctcc
    18181 ggggaaccgg ttcgagacac catggtttat tgatactcgc ggcgcactac tatggagatg
    18241 ggcctcgaac gtgacgtgca aagggcagta gatcggatct ctgaagccga ctcggtgacg
    18301 atcatctccc acatcgacgc cgacggcatc agcacggagt cgatacttgc gcaggcgctc
    18361 gcccgtgaag gtatacccgc cacttccgtc ttcgtccgcc agctcgagcc gatggcgatg
    18421 cgccacatcc cggatgacga ttccctgaag atcttcaccg acctcggggc cggccagcag
    18481 gcccttcttg aggagcacgg cctctctgcg gatggcgtcc tgatcgtcga ccaccacgtc
    18541 agccagccct gcggcacggc ctatccccag gtgaactgcc ttgacttcgg catcacccgg
    18601 atgagcgccg cgggcatcgc ctacatggtg gcgagggcga tcgattcggc aaacaccgac
    18661 cttgcgaagc ttgccgtcgt cgggaacgtc ggcgacatga tggcccgcga gaactgtgga
    18721 ctggtcggtc cggcgcgcga gatcgttcag gacggcgtgg agtacggcaa cgtaatcgtg
    18781 cagagagcgg acctgaactg ctatgggata tccacccgcc ccgtccacgt ctgcctcggc
    18841 cgctgcgacg acccttacat cgacggtata tcaaacagcg tgacccgggc gctccggttc
    18901 cttgagaggc tgggtgttac gctgaagacg ccgcggggcg gctggctcgt ctgggaggag
    18961 gttccgctcg atgaccggcg cacgatcatc agcgccctta cccagcagct cgtcgcgcac
    19021 ggaagagagg ccgaccgcct cctcgccgag acctacatct tccccgacga accggaacgg
    19081 acaccgttcc ggaacgcctc cgagtacgcc accctcctga acgcctgcgg ccggtgggca
    19141 aaaccgaagg tcggggggcg tatctgccgc ggagaccgcg gggatgccta ccgtgaggca
    19201 gagtacatgc tcgcccacca ccgctcggtc attcgcggcc tgctccagta catactcgag
    19261 accggggtga cggagctctc ccacctccag tacatccaca ccggcgaccg gttccccgac
    19321 accatcgtcg gtatcggggc gggcatggcg ctctccaaac tgaactggaa gaaaccgatc
    19381 atggtgctcg ccgcgatggt ggatgagccg gatgtcacca aggtctcgat gcggacgaac
    19441 gaatgggcgc tcgcccgggg agtcgacctg caggcggcgc tcgtcgaggc atcggcaacg
    19501 gtgggcggcg cgggcggggg gcaccggatc gcggccgggg cgttcatccc ccacgacaca
    19561 gaagaggagt ttatcgatgg tatcaaccga atactcacgc gacagttcgc ttcggcggat
    19621 acggacaata gctgacttcc agttcggcgc cgggtcggga gaggccctct ttcccgaagg
    19681 gtgcacgttt gagttctcga cgaccggacg catccgccag gtgcgcctcg ggaacgagag
    19741 actcgtgacc gtccgggcac aggacggccg cctgaccctc gggatcgccg gagcggcgcg
    19801 ccttgccgct gttctcgccc ccccgaccta ccgggtgacg gtgcgggagg atgtggcggc
    19861 gttcatcgca gacgggaaga atgcgatggc aaagcacgtc gtcgctgccg atgagggtat
    19921 ccgtgcaggg gacgaggtgc tggtggtgac cggggacgac atgctccttg ccaccggtgc
    19981 agccctgctc tccggccggg agatactggc atttaattac ggtgtagcgg taaaagtacg
    20041 acagggaaga ggatccgaat gtttccaggc aagataaacc cgagaaaaat gaagcagatg
    20101 atgaagcaga tgggcatgga gatggaggaa cttgagggcg tcgagaaggt ggtgatctac
    20161 acgcccgccg ggaactacgt ctttgacgat gcccaggtcg tcgcgacaac gatgcagggg
    20221 gtcaccacct accagctcac cggggaggcc cggtttgaag aggcggtccc ggagattccc
    20281 gacgaggacg tcgacctcgt cgcatcccag gccggtgttg ccggagaggt cgcgaagaag
    20341 gcgctcatcg agacccgcgg cgacatcgcc gaagctatcc tgaagctcac gcagcaatga
    20401 tcgagaccgg cgaacagatc ctgctcgtcg gcgaggaccg cgagtacttc gtgacggcgg
    20461 gcgaggggca gtt
//
LOCUS       DAOSEE010000023        19702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1154_length_19702_cov_251.992365, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000023 DAOSEE010000000
VERSION     DAOSEE010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 19702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19702
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1154_length_19702_cov_251.992365"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="PLI76_03125"
     CDS             <1..824
                     /locus_tag="PLI76_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOI61052.1"
                     /translation="NVDAIVYVRVIDPEKAYFEVMNYRAATVALAQTSLRGIIGDMEL
                     DEVLYNRDIINARLRDILDRETDAWGVKVERVEIKEVDPVGPVKQAMTEQTAAERERR
                     AAILRADGEKRAAILKAEGSRQSIILEAEGERQSKILRAEGERMSRILQAQGEAQGLR
                     IISVGARPLDKRAITVLSLDALKQMAEGQATKIIFPFELSTIVRQAAEYLGATVEAPD
                     VPEGTQMPSEDILGEIPSKEELRAVEEAAKAPDIDVEKETRKKAQELAKEEGEGL"
     gene            955..1950
                     /locus_tag="PLI76_03130"
     CDS             955..1950
                     /locus_tag="PLI76_03130"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOI61053.1"
                     /translation="MTETTPAFAEKSFFINETGQELPLEDFVPVEILEMAEIEGQRAI
                     PGIAKNISLMKEIEAISSNVPTRHRFMHADSRNLAFLPDESVHLVVTSPPYWTLKKYP
                     ERDEQLGQIEDYETFLEELDNVWRHIFRILVPGGRLVIVVGDVCLPRREAGRHVVMPL
                     HASIQEHCRRIGFDNLAPVIWYKIANACFEADSPGSFLGKPYEPNGIVKNDIEYILFQ
                     RKPGGYRKPTLAARLLSVISEENQKVWFRQIWDLKGASTREHPAPYPQELAERLVRMF
                     SFVGDTILDPFGGTGTTALAAARWGRNSISAEVEEKYFLMQVERFAHQAKQSRLF"
     gene            1964..2731
                     /locus_tag="PLI76_03135"
     CDS             1964..2731
                     /locus_tag="PLI76_03135"
                     /inference="COORDINATES: protein motif:HMM:NF016442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaeR7I family type II restriction endonuclease"
                     /protein_id="HOI61054.1"
                     /translation="MVSDRILREISRDDYFSCAVRYFWDKRASQAKDQDNRGAKDQGF
                     RSEATGGKHMDRFFSLIRDLLVDVGVPEGDVCIRKRSLNLPGYFRAEKQWDLIVIKHH
                     PDGKKELVAVLELKSQKGPSFGNNINNRTEEVLGSAYDLWTAYREGAFKTSPSPWLGY
                     LLLLEDCERSRRSVRNNEPHFGVFPEFKEASYIERYHQTCLKLVRERVYSEVCYLLAK
                     EEDKMQLRNYSEPDGTLSGYRFLRSLCSHLNNFYEVT"
     gene            complement(2826..4157)
                     /locus_tag="PLI76_03140"
     CDS             complement(2826..4157)
                     /locus_tag="PLI76_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOI61055.1"
                     /translation="MTWLQILLLLVAAYLLIAYYIRDRGLFRDHVMFFGPIMAIKTER
                     VGFFDWFRKFQMPLRAYATLGVIMVVLVSAFMTLALLLAVPQLLVHTPEPTGIYDPRN
                     ILAIPGVNQAIPVTAAVIIGLVLTIVIHEFGHAILARVEGMRVRSMGLLIAVIPIGAF
                     VEPDEEDVDAAKGMPKIRMFGAGITNNIVIGLACFAVMFLLVGMATPLAVPLVQGVYK
                     DFPAADAGLPGYSVITSINGVPVASQAEVSAAMEGTRPGETVTLTAEKDGIESTYTLT
                     LSRWPEVLDGDHDSGFMGVYYYSAPVVKEHIGNIANLGLLAPLYLTIAPIDAFIQGNT
                     QQLAILLTDTPEQMAWKEPFPFFWGMVHLAFWTGWFNLLVGMFNAIPIVPLDGGYMMK
                     EGVERFLERRGWSRYAPRVVASISGFVTVMLILLITMPMLVAAVRFVLMMG"
     gene            complement(4198..4827)
                     /gene="rnhB"
                     /locus_tag="PLI76_03145"
     CDS             complement(4198..4827)
                     /gene="rnhB"
                     /locus_tag="PLI76_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOI61056.1"
                     /translation="MICGVDEAGKGSVIGPMVVAAVGCRAVEDLAALPIRDSKALRPK
                     QREELYGLIFRDFAIAIVTIDAAGIDEARSRMSMNACVAELHAEALMGLRPEHAYVDA
                     CDVNAERYGRTVTSHLDFPCAVVAEHRADARHKIVGAASVIAKVTRDRAIRELEQQYG
                     RIGSGYPSDPATIEFLRNYMREHGNPPPCARRSWKTVANLYQATMQDYL"
     gene            4882..5547
                     /locus_tag="PLI76_03150"
     CDS             4882..5547
                     /locus_tag="PLI76_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOI61057.1"
                     /translation="MYTIIVGLGGIGRNLTAIAVNAGDSVVVIDQDEARASDILEHYD
                     VLAITGNATDKSVLQDAGIDRADALVATTSDDAVNLMTCWLAKRYNVRNVVSIVNQKE
                     HSDLFNEVGVKISENPDELVASRLYYWAKSPNLQQVASIPGGTIFEIVAEEGAPIADH
                     EIRDLDVRDFVFIAIRRAGGDLIIPSGTVRIRPGDVITVFTKKEAEGETVKTLSKQLK
                     RSG"
     gene            complement(5539..6735)
                     /locus_tag="PLI76_03155"
     CDS             complement(5539..6735)
                     /locus_tag="PLI76_03155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOI61058.1"
                     /translation="MKSNYDILVVGGGPAGALAAKTAAEAGNSVCLIEKRSAIGTPVR
                     CAEGIGKELLREFVKPDPRWISAEIERARLIAPNGTTVNLEQDRAGNEVGYVLDRKIF
                     DRELVWQAAEAGADVVVKTRATAPIQENGVVRGAKVLSVGTPADIRADVVIAADGTEA
                     QFARRAGLDTTVPLREMMSCAQYLMTDIDIDAGSTDFYLGNDVAPEGYLWIFPKGERT
                     ANVGIGISGRKSRDGTRAKDYLDRFVAKNFPDGKTIECIAGGVPVCRPLPCTVGDGLM
                     VIGDAARVVDPLTGGGIGNAMFTGRLAAQVASECISAGNCSKEALMPYDAGWRASRMG
                     VLLERNYRVKEYFVTLDDRKLNTLAESIANISLKEFSVSALIKELIKRNPKMLLELKA
                     LKDALS"
     gene            complement(6725..6901)
                     /locus_tag="PLI76_03160"
     CDS             complement(6725..6901)
                     /locus_tag="PLI76_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOI61059.1"
                     /translation="MLRINRDKCGYCGTCVAVCPEDALELIDAYLSLERECIACGICA
                     RACPFGALEVVHEE"
     gene            7077..7607
                     /gene="cbiT"
                     /locus_tag="PLI76_03165"
     CDS             7077..7607
                     /gene="cbiT"
                     /locus_tag="PLI76_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6Y C5,15-methyltransferase
                     (decarboxylating) subunit CbiT"
                     /protein_id="HOI61060.1"
                     /translation="MGLKGGPTQDEVMAVSLAKLGIRPGDRVADVGCGTGKVAVAASK
                     VAGQVYAIDRRPEAIACARQEAVAAGAGNIEFFEGDAVDILPGLGRLDAAFVGGSRRL
                     PEVLDILAGKVQGRIVVNAVMVGTLQSAIESMRRLGIFSEAVHLQVSRSADIAGGVMF
                     KPINPVYIVVGGAGCS"
     gene            7598..8215
                     /locus_tag="PLI76_03170"
     CDS             7598..8215
                     /locus_tag="PLI76_03170"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HOI61061.1"
                     /translation="MLVGVGLGPGDPELLTLRAVRLLREAAAVFVPGKLAYDLVRPYR
                     PDAVVLNFPMTSDEAYIRECLEENADRIAPHARGGLAVFGIIGDPNFYSTFSRLCDVI
                     GERYPEIAFSTEPGISSITAFASVANVPVAGGFFVSDGNGPESRIFMKVRRPAALAAS
                     LAKEGYAEFVLVERMFLPGQQVYRGDDLPEESDYFSILYARRLNA"
     gene            8208..8924
                     /locus_tag="PLI76_03175"
     CDS             8208..8924
                     /locus_tag="PLI76_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4/precorrin-4
                     C(11)-methyltransferase"
                     /protein_id="HOI61062.1"
                     /translation="MHKVYIVGAGPGAPDLITVRGMDLLRRADVLIYAGSLVNPALVE
                     ESGAGLKLDSQKMTLEDIADAVEEHVRAGELVVRLHSGDPSLYGAIVEQMAEFSRRGI
                     EAEIVPGVSSLFAAAAALKTQLTLRDVSESLIVTRPAGATLAEDRIAEFSRLGQTMVV
                     FLGTERMEEVLSRVECPPETPAAVVYHASWPDQKVVTGTVADIAAKARAAGIERTALI
                     IIGKVADPATSGFGRSVLYS"
     gene            8921..9793
                     /gene="cbiG"
                     /locus_tag="PLI76_03180"
     CDS             8921..9793
                     /gene="cbiG"
                     /locus_tag="PLI76_03180"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HOI61063.1"
                     /translation="MTGTVVVALERFLPDARRIADMLGADTIPYGPDAFREAFCRYRR
                     IVALMSAGIAVRGIAPLLADKWRDPAVVVVGPDLRYAVPVVGGHHGANDLARELARLG
                     IEPVITTATETRGRESVERIAARSGCDIVNRDSTRAVNAAVLDADVPLYAIAGPGIVV
                     AGPEVSLLVRKGEYVVGVGCRKDVPAADVTRAVGEAFREAGVAPAEVLVYATTEKKRG
                     EAGLLAAVADLGGNLVFLDDETLNAEAAPSPSRASLIGLAGVAEPAALAVSKRKELVF
                     AKQTYGSVTVAIAR"
     gene            9762..10556
                     /gene="cobJ"
                     /locus_tag="PLI76_03185"
     CDS             9762..10556
                     /gene="cobJ"
                     /locus_tag="PLI76_03185"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HOI61064.1"
                     /translation="MGVLPLQSHDNGGKLYIVGTGPGELLQMTPRASKALEEADCIIG
                     NGFYLDLLGPMLAGKEVIRSGMGREVDRASRALALAGERVVAMVSGGDAGVYGMASIV
                     LEVAERSGTKVAVEVIPGITAAISAAALLGSPLSGDYVTMSLSDLLTPGEEIERRLDL
                     AFQMRVPVVLYNPRSRGRPHNLDAAVAIARRHLSDATPVGVVRNAYREGEERLITTLG
                     EFEDHFAFVDMHSIVFIGGEETRIWRDETDERGIITPRGYHRKYVY"
     gene            10504..11184
                     /locus_tag="PLI76_03190"
     CDS             10504..11184
                     /locus_tag="PLI76_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HOI61065.1"
                     /translation="MREESSRPGDTTGSTYTDLAAVTPEAYAIARTSRNLARERVGNV
                     TLEDRVRQRCSVAVGDFAMADLMRFSGDPVTAGLAALDRRVPIITDIRMVQAGILKRG
                     HESEILCALDYGEDIARERGITRTSAGFVALQERVEGAIVVIGNAPSALLVVCDMVRA
                     NIRPALAIGTPVGFVNAAESKEALRTLDVPSVSNAGTRGGTPVAVAAMNEIITIFAER
                     AGYEGSGY"
     gene            11165..12181
                     /locus_tag="PLI76_03195"
     CDS             11165..12181
                     /locus_tag="PLI76_03195"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HOI61066.1"
                     /translation="MKDPVTDFEYPGAWVAACRSPDLLPDVRRGLAVLTASGTVLRRG
                     FTTGTTAAAACKAAVLSLAEDEVKGVGITLPCGIAVRVPVDAYRGTASCWKDAGDYPA
                     DVTAGLEFVATAVPSLSGSVQFVPGEGIGSFARDTPRHRQGTPAISAPALDCIRRSID
                     EAVEEAGLHGATVILTIPRGAEVAQKTLNPKVGVQAGISVLGTTGLVEPWDDHLAEGV
                     LDRIARALPGAVVLTTGRLGLRYSRLLFPEHEAILVGSNLSEALRAVDGDAVLCGLPG
                     LILKFMNPAVLDGTGCATVEELSGSPLWEEVARREVRAFSVRYPRVRVVIVDRGGRVI
                     AESP"
     gene            12178..12750
                     /locus_tag="PLI76_03200"
     CDS             12178..12750
                     /locus_tag="PLI76_03200"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HOI61067.1"
                     /translation="MKIVGVGCGPGMLTAEAARTLRGAALIYGSARAIALARDVFPPG
                     CEVHEVADYRDLRSLPDRAVVLSTGDPMLAGLGYLPGDVIPGISSLQVAFARLKIPLT
                     KAAVVTAHGKDHARAVADAREEVLRGRVVFLIADPAFDVQAFAAALPPETGVAVCEDL
                     GYPAERIAVGTGAEPPAPRGGLFVVVAGEF"
     gene            complement(12772..13095)
                     /locus_tag="PLI76_03205"
     CDS             complement(12772..13095)
                     /locus_tag="PLI76_03205"
                     /inference="COORDINATES: protein motif:HMM:NF024736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOI61068.1"
                     /translation="MTFREIDDETWEIIRRHLPPRKPHVGRPRRDPRHLFNGILYVLS
                     TGCTWSDVPAKYGTKSTVHRYHLELCERGAYQAIFLDLLRSGYEFRKREVDRPIVRNL
                     RAPAL"
     gene            complement(13184..14122)
                     /locus_tag="PLI76_03210"
     CDS             complement(13184..14122)
                     /locus_tag="PLI76_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HOI61069.1"
                     /translation="MATYTARPFLKWAGGKAQLLDAFTRRIPRELSEGTLPVFVEPFV
                     GGGAVYFHFNSTFRFRECHIFDINEELILTYSVVKSDVCALIDYLAGISDDYLTKDDL
                     GRKDFYYAMRDTFNRAKETIDFGQYGEGWIERAAQLIFLNRTCFNGLYRVNLQGGFNV
                     PFGRCRNPTILNEDVLRADAELLRNTTIHHDDFAQSELYVSKDTFVYLDPPYRPLNRT
                     STFTQYAKEGFTDEEQRRLAAYYARCDARGARLMLSNSDPKNVDPCDEFFDDLYAGYR
                     IDRVPARRMINCNGDGRGEIREIIVTNYDPSAPGER"
     gene            complement(14322..15353)
                     /locus_tag="PLI76_03215"
     CDS             complement(14322..15353)
                     /locus_tag="PLI76_03215"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOI61070.1"
                     /translation="MRSEEINGNIFYNGDCIRGAREHIPDGTIDLIVTDPPYGIDGDR
                     LHRHYNRDEAFVVEGYVEVPAAGYGEFSRQWIREAGRILRPGGSIYIVSGYTNLYHIL
                     HALRQTELREVNHIIWKYNFGVYTSRKYVSSHYHILFYEKPGGKRTFNLESRYGTGEK
                     APDNGSPNYRDREDVWVVNREYKPGKAKNKNELPAELLVKMLQYSSNEGDLVCDMFLG
                     GFSTARAAVGLNRRATGFELSGPVFDLKVKELRGIGQGCLLPSLRTPLASGPKNRGRP
                     WTEDEIEALVSRFARLIEGGATKKKAVDTLAEELCRGRWAIEKMLKREGVRRTGRGAT
                     TPAPRSREE"
     gene            15678..17318
                     /locus_tag="PLI76_03220"
     CDS             15678..17318
                     /locus_tag="PLI76_03220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOI61071.1"
                     /translation="MTAGSDVTETSSRMGAEEREQKTSAMEEGPVHECIAAILAENEA
                     LRRENALLQSMKNALEEGEMRYRRLFEDALTGDFITAPDGRIIACNPAFARMFGFSSV
                     EGALCTNVRDLYEDPGDRDRLLAQLRRGGRVVNEGRTRKRRDGTRIHVVENVVGRLGA
                     DGELIEIQGYTYDDSERKRVEDALRESEERFRAVLENSLDSAYRRNLQADCYDYMSPA
                     IEEILGFTPEEMAVMSLEEVMDRIHPDDRSLVKAELNTAAASGRGLLVYRFLAKGGEY
                     RWLEDHFTVISDSAGRPLFRGGIVRDITGRRRAEEAIARSAAELARVHRDLESAHREA
                     NLYLDILTHDIGNTENVSNLYAELLAGSVEGEAARYAANLKRSIEKSIEILRTVSKIR
                     RIHAGPPGLRPIDLDAVVRAEIAHFPGIPIRYEGAPRTVLADDLLCEVFTNLIGNAVK
                     HGGPGVAVIIRVEAEEEGLLRVTVADTGRGVPEERKEEIFHCYERKQRGVGEGLGLYL
                     VRILIDRYGGRIRVEDRIPGYPGEGAAFSFLLREAERS"
     gene            17422..19554
                     /locus_tag="PLI76_03225"
     CDS             17422..19554
                     /locus_tag="PLI76_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOI61072.1"
                     /translation="MMSEQEQRRIPVRTKILLVFLGLSMAALLLAGSLAFVQMDDVSR
                     YALARSTDLGARAVNDSTAALERNAEESLLRLAQDQAYISTILFEQVNGDLEVMGRYA
                     ATVMANPSMVRPRHFYLQDEEPEDRRATSVLFLSPGVDGGRLRDERNAAGMMDDIFIP
                     VSATNRHLAGVYVGTGSGMAMIYPWTTGLDATCDPRLRTWFVQAVETGETVWSEPYVD
                     LIGHGLMVTCSQPVYGPERDWVWVVGADVTVEMIDRQIVGTQVGDRGYAMLIDQHGNV
                     ISRPGLTSGDMRWDESFITENLLASENPDIVAVAKKMVAGETGIARVGFEDGERFVAY
                     APVRGVNWSVGVVMPVDEVLAPITTTRASILSASEDTALHIGAQQDRMKAVFTGAFVV
                     LLVVVALLTLAVTRYFTRPIEELQRGSEAIGRGDLEHRVNVATGDEFEDLARSFNRMA
                     ADLRGHIEDLRRTTAEKERIEKELEIAKEIQQSILPESAPVLPGFDIAGFNLPAREVG
                     GDFYDYIPVGEGRWGVEIADVSGKGVPAALFMALSRTLVRASASENPDPAGSILEANR
                     YICMDSKTCMFVTLFYGILDTRKGTFTYVNAGHNPPLLFRAGSSTAELLRGKGIALGI
                     FDDIELELVELRLNAGDTVVFYTDGVTEATNERDEEYGMEQLTALIPGLLDLGARGMI
                     DAIVADVTAFAGDRPQFDDITLVVLKVG"
BASE COUNT         3502 a         6000 c         6489 g         3711 t
ORIGIN      
        1 cgaacgtgga tgcgatcgtc tacgtccggg tgatcgaccc cgagaaggcc tacttcgagg
       61 tgatgaacta ccgggcggcg acggtggcgc tcgcgcagac gagtctccgg ggtatcatcg
      121 gcgacatgga gcttgacgag gttctctaca accgcgatat catcaacgcc cgtctccggg
      181 atatcctgga tagagagacc gatgcatggg gcgtgaaggt cgagcgggtg gagatcaagg
      241 aggtcgatcc ggtcgggccc gtcaagcagg cgatgaccga gcagaccgca gccgagcggg
      301 agcggcgcgc ggcgatcctc agggcggacg gcgagaagcg ggccgcgatc ctcaaggccg
      361 agggcagccg gcagagcatc atcctcgagg ccgagggcga gcggcagagc aagatcctca
      421 gggccgaagg cgagcggatg tcgcggatcc tgcaggccca gggcgaggcg caggggttgc
      481 gcatcatatc ggtcggcgcc cggccgctcg ataaacgcgc gataacggtc ctctccctcg
      541 atgccttgaa gcagatggcc gagggccagg caacgaagat catattcccg ttcgagctct
      601 cgacgatcgt caggcaggcc gccgagtacc tcggtgccac ggtcgaggcc ccggatgtcc
      661 ccgagggcac acagatgccg tcggaggata tcctcgggga gataccgagc aaggaggaac
      721 tccgggccgt tgaagaggcg gcaaaggccc ctgacatcga tgtcgagaag gagacgcgga
      781 aaaaagccca ggaactggca aaggaagagg gagaggggct gtagggcatt ccccggcccc
      841 tgctattttt ggggcagtag gtctccgggt ccgcaaggga gagatttccg gtgcagttat
      901 gtgttcgagg gtatcaatca tagaagcggt agcatgatct caaaaccatt tcatatgacg
      961 gaaacaaccc ctgcttttgc cgaaaaatcc ttttttatca acgagaccgg gcaggagcta
     1021 ccactggaag actttgtacc ggttgagatc cttgagatgg cggagatcga ggggcagcga
     1081 gccataccgg ggatagcgaa gaatatctcg ctgatgaaag aaattgaagc catatccagc
     1141 aatgtgccga cacggcaccg tttcatgcat gcagatagcc gaaaccttgc gtttctccct
     1201 gatgaaagtg ttcatctggt cgtgacgtcg cctccttact ggaccctgaa gaaatacccc
     1261 gaaagagatg aacagctcgg gcagatcgag gattacgaga cctttctgga agaactcgat
     1321 aacgtctgga ggcatatctt ccggatcctt gtccccgggg gacgcctggt catcgtcgtc
     1381 ggcgatgtct gcctgcccag gcgggaggcc gggcgccatg tcgtcatgcc cctccacgcc
     1441 agcattcagg agcactgccg gaggatcggc tttgataacc ttgccccggt catctggtac
     1501 aagatagcaa acgcctgttt tgaagcagat tcacccggat cgttccttgg caaaccgtat
     1561 gagcccaacg ggatagtaaa aaacgatatc gagtatatcc tcttccagag aaaacccggc
     1621 ggttaccgga aacccacgct tgcggcacgt ctgctctcgg tcatatcgga agagaaccag
     1681 aaggtgtggt tccgccagat ctgggacctc aagggagcgt cgaccagaga gcaccctgca
     1741 ccctatcctc aggaactggc tgagaggctt gtacggatgt tctcgttcgt gggtgatact
     1801 atcctcgatc ccttcggcgg cacggggaca acggccctcg ctgccgcgcg gtgggggcga
     1861 aacagcatca gcgccgaggt tgaggagaaa tatttcctca tgcaggttga acggtttgct
     1921 caccaggcga aacagtctcg attattctga tagaggtggg tccatggtga gcgacaggat
     1981 acttcgggaa atctcccgtg acgattattt ttcctgcgcg gtgcggtatt tctgggataa
     2041 gagagcatca caggcaaaag atcaggataa ccggggtgcg aaagatcagg gctttcgctc
     2101 ggaggcgaca ggcggaaagc atatggaccg gtttttctcg ctcatccggg atctgctggt
     2161 ggatgtcggg gtgccggagg gtgatgtctg catacggaag agatctctta atcttccggg
     2221 gtacttccgc gcggagaagc agtgggatct tatcgttatc aaacatcacc ctgatgggaa
     2281 gaaggaactg gttgccgtcc ttgaattgaa gtcgcagaag gggccgtcat ttgggaataa
     2341 catcaataat cggacggagg aggttcttgg gagtgcgtac gatctctgga cagcctaccg
     2401 ggagggagct ttcaaaacgt ccccgtcacc ctggcttgga tacctgcttc tgctggagga
     2461 ttgtgagaga tcccgaagaa gcgtacggaa caacgaacca cactttgggg tttttcctga
     2521 gtttaaagaa gcctcttata tcgagcgcta tcaccagacc tgtctcaagc tggtacgcga
     2581 gcgagtctat tccgaggtct gctacttgct tgcaaaggaa gaggataaga tgcaactccg
     2641 gaactattcc gaaccggacg ggactctgtc aggatatcgg ttcttacggt ccttgtgttc
     2701 ccatctgaac aacttttacg aagtaacata gcgtcatcag gcagtggggc aaccactatg
     2761 atactgctgt tggaatccga cgatgcgaga atccccctat gccgccccga tgagaaaaag
     2821 ggtcttcacc ccatcatcag cacaaaccgc accgccgcga caagcatcgg cattgtgatg
     2881 aggaggatca gcatcaccgt gacgaatccg ctgatcgagg cgacgaccct tggtgcgtac
     2941 cgggaccagc cccggcgctc gaggaaccgc tccacccctt ctttcatcat ataaccgccg
     3001 tcgaggggga cgatcgggat ggcgttgaac atgccgacca gcaggttgaa ccaccccgtc
     3061 cagaaggcga ggtgaaccat gccccagaag aagggaaacg gctccttcca ggccatctgc
     3121 tcgggcgtat cggtaagcag gatcgcaagc tgctgggtgt tgccctggat gaacgcatcg
     3181 atcggggcta tcgtcaggta gagcggggca agcagcccga ggtttgcgat gttcccgata
     3241 tgctccttca ccaccggggc gctgtagtag tagaccccca tgaaccctga atcgtggtcg
     3301 ccgtcgagca cttccggcca tctcgagagg gtgagggtgt aggtgctctc gatcccatcc
     3361 ttttctgccg tcagcgtgac cgtctccccg ggccgcgtcc cctccatcgc cgccgagacc
     3421 tccgcctggc ttgccacggg tacaccgttt atgctcgtga tgaccgagta gccggggagg
     3481 cccgcgtcgg ccgccgggaa atccttgtag accccctgga cgagcgggac ggcaaggggc
     3541 gtcgccatac cgacgaggag gaacatcacg gcgaaacagg cgaggccgat gacgatgttg
     3601 ttcgttattc cggcaccgaa catccggatc ttcggcatcc cttttgccgc atcgacgtcc
     3661 tcttcgtcgg gctcgacgaa cgcccctatc gggatgacgg cgatgaggag acccatgctc
     3721 cgcacccgca taccctcgac ccgggcgagg atcgcgtggc cgaactcgtg gatgacgatc
     3781 gtgagcacga ggccgatgat gacggccgcg gtgaccggta ttgcctgatt gacccccggt
     3841 atggcgagga tgtttctcgg atcgtagatc ccggtcggtt cgggggtatg caccaggagc
     3901 tgggggactg caaggagcag ggcgagtgtc atgaaggccg agaccaggac gaccatgatg
     3961 accccgagcg tcgcgtaggc ccgcagaggc atttggaatt tccggaacca atcgaagaaa
     4021 ccgacccgct cggtcttgat cgccatgatg gggccaaaga acatgacgtg atcgcgaaac
     4081 aatccccggt cgcggatata atatgctatc aggagatagg ccgcgacgag aaggaggagt
     4141 atctggagcc aggtcatcac tcacgattag cgcgcagaaa gcataaattc ttccgggcta
     4201 caggtagtcc tgcatcgtcg cctggtagag gttcgccact gtcttccagc tccggcgggc
     4261 gcagggcggc gggttcccgt gctcacgcat gtagttccgg agaaactcga tggttgccgg
     4321 atcggacggg tagccgctcc cgatcctccc gtactgctgc tcgagttccc ggatcgcccg
     4381 gtcccgggtg accttcgcga tgacgctcgc cgcgccgacg atcttgtggc gggcatccgc
     4441 ccggtgctcg gcgacgaccg cacaggggaa gtcgaggtga gacgtgaccg ttctgccgta
     4501 acgctcggcg ttcacgtcgc aggcgtccac gtaggcatgc tccgggcgga gccccatgag
     4561 cgcctcggca tggagttcgg ccacgcacgc gttcatgctc atccggctcc gggcctcgtc
     4621 gatcccggcg gcgtcgatgg tgacgatggc tatcgcgaag tcgcggaaga tgagcccgta
     4681 cagttcctcg cgttgtttcg gccggagggc ctttgagtcc cggatgggga gggccgcaag
     4741 gtcctcgact gcccggcacc cgacggcggc gaccaccatc gggccgatga ccgaaccctt
     4801 ccccgcttca tccaccccgc agatcacgta ctctggttta ttcgcaaggt atttcaatgg
     4861 caacggtcga acccagatgg aatgtacacc atcatcgtcg gccttggggg gatcggccgg
     4921 aacctgactg cgatcgccgt gaacgccggc gactccgtgg tggtgatcga ccaggacgag
     4981 gcgcgtgcaa gcgatatcct ggagcactac gacgttctcg ccatcaccgg gaacgcgacc
     5041 gacaagtcgg tgctgcagga tgccgggata gaccgtgccg acgctctggt cgccaccacg
     5101 agcgacgacg ccgtgaacct gatgacctgc tggcttgcca aacgttacaa cgtgaggaac
     5161 gtcgtctcga tcgtcaacca gaaggagcac tccgaccttt tcaacgaggt cggggtgaag
     5221 atcagcgaaa accccgacga actggtggcg agccggctct actactgggc aaagagccca
     5281 aacctccagc aggtcgcctc gatccccgga ggcaccatat tcgagatagt cgctgaagag
     5341 ggcgctccca tcgccgacca cgagatccgc gacctcgacg tccgcgactt cgtcttcatc
     5401 gccatccggc gcgccggggg cgacctcatc ataccgagcg gcaccgtccg tatccgcccg
     5461 ggagacgtca tcacggtatt tacaaaaaag gaggcggagg gggagacggt aaagaccctc
     5521 agcaaacagc tgaagcggtc aggataaagc atctttgagc gctttcagtt cgaggagcat
     5581 cttcgggttg cgcttgatca gctccttgat gagcgccgag accgagaatt ccttgagact
     5641 gatgtttgcg atcgattccg cgagcgtgtt gagtttccta tcgtcaagcg tgacgaagta
     5701 ctccttgacc ctgtagttcc gctcgagaag tacgcccatc cgcgatgccc gccacccggc
     5761 atcatagggc atcagcgcct ccttcgagca gttgcccgcc gatatgcatt cggaggccac
     5821 ctgagccgcc agccttccgg tgaacatggc gttcccgatc ccaccgccgg tgagagggtc
     5881 gacgaccctt gccgcgtcgc cgatgaccat aaggccgtcg ccaaccgtgc aggggagcgg
     5941 ccggcagacc gggacgccgc ctgcgatgca ctcgatcgtc tttccgtcgg ggaagttctt
     6001 tgcgacgaac cggtcgaggt agtcctttgc ccgcgtgccg tcccggctct tcctgccgga
     6061 gatgccgatc ccgacgtttg ccgtccgctc gcccttcggg aagatccaga ggtagccttc
     6121 gggagcgaca tcgttaccga ggtagaagtc cgtcgaaccc gcatcgatat cgatatcggt
     6181 catcaggtat tgagcgcagc tcatcatctc gcggaggggc acggtggtgt cgaggccggc
     6241 ccggcgggcg aactgcgcct ccgtgccgtc cgcggcgatg accacgtcgg cccggatgtc
     6301 ggccggcgtc ccgacggaga gcacttttgc gccccggacc acgccgttct cctggatcgg
     6361 cgcggtcgcc cgggtcttga cgaccacgtc cgcccctgcc tcggccgcct gccagacgag
     6421 ttcccggtca aagatcttcc tgtcgaggac gtagccgacc tcgtttcccg cccggtcctg
     6481 ttcgaggttg acggtggtgc cgttcggggc gatgagccgg gcacgctcga tctccgccga
     6541 tatccagcgg gggtcgggtt tgacgaactc ccgtaagagt tctttgccga tcccctccgc
     6601 gcaccggacc ggtgtgccga tggcggagcg cttctcgatg aggcagaccg agttccctgc
     6661 ttctgccgcc gtctttgccg caagagcgcc ggcgggtccg ccaccgacga cgagaatatc
     6721 gtagttactc ttcatggacg acctccagtg ccccgaacgg gcacgcccgt gcgcagatgc
     6781 cgcaggcgat gcactcccgc tcaaggctga ggtaggcatc gatcagctcg agcgcatcct
     6841 ccgggcagac tgcgacgcag gtgccgcagt atccgcactt gtctcggttt atcctgagca
     6901 ttgtactcat gttgtcgttg gtctcgcaaa tacctttatg ttctctgttt ctggcggcgc
     6961 acgacttccc cacctggagg gaggaacggc ggatcgctcg ccgtgattca tggagtcccg
     7021 ttcactgggc tattataata gatgttagca caaatttatt gggaaatggg gatgcaatgg
     7081 gactgaaggg tggccctaca caggatgaag tcatggcggt ctcgctcgca aaactcggta
     7141 ttcgcccggg cgaccgggtg gccgacgtcg ggtgcggaac cgggaaggtt gccgtcgccg
     7201 cgtcgaaagt ggcgggccag gtctacgcga tcgatcgaag gccggaggcg atcgcctgtg
     7261 cccggcagga ggcggttgcc gccggcgccg gaaatatcga gttcttcgag ggcgacgcgg
     7321 tggatatcct cccgggtctc ggccggctcg acgccgcctt cgtcgggggg tcccgccgcc
     7381 tgccggaggt cctcgatatc ctcgccggga aagtgcaggg gcggatcgtc gtgaacgcgg
     7441 tgatggtcgg gacgctgcaa tcggcgatag agagcatgcg acgcctcggc atcttctcgg
     7501 aggccgtcca tctccaggtc tcccggtccg ccgatatcgc cggcggggtg atgttcaagc
     7561 ccatcaaccc cgtctacatc gtcgtcgggg gtgccgggtg ctcgtagggg tggggctcgg
     7621 gcccggcgac cccgaactcc tgaccctgcg ggcggtccgg ctccttcgcg aggcggcggc
     7681 ggtctttgtc ccgggaaaac ttgcttacga cctggtgcgg ccctaccgcc ccgacgccgt
     7741 cgtcctcaac ttcccgatga cgagcgatga ggcctatatc cgggagtgcc tcgaggagaa
     7801 cgccgaccgg atagcgccgc acgctcgggg cggtcttgcg gtctttggga tcatcgggga
     7861 tccgaacttc tactcgactt tttcccgtct ctgcgatgtg atcggcgagc gataccccga
     7921 gatagcgttc tcgaccgagc ccgggatcag ctcgatcacc gcgttcgcct ccgtggcaaa
     7981 cgtcccggtg gccggcgggt tcttcgtctc ggatgggaac gggccggagt cccggatctt
     8041 catgaaggtc cggcggccgg cggcacttgc ggcctccctc gcaaaagagg gctatgcgga
     8101 gttcgtcctc gtggaacgga tgttcctccc cggtcagcag gtctaccggg gggacgacct
     8161 ccccgaggag agcgactact tctcgatcct ctatgcgagg cgactgaatg cataaagtct
     8221 acatcgtggg tgcggggccg ggagcccccg atctcatcac cgtccggggc atggacctcc
     8281 tccggcgggc ggacgtcctc atctatgcgg gttcgctcgt gaacccggcg ctcgtggaag
     8341 agtccggtgc cgggctgaaa ctcgatagcc agaagatgac gcttgaagat atcgccgatg
     8401 ccgtcgagga gcacgtccgt gccggagagc tcgtcgtccg gctccattcg ggcgatccgt
     8461 cgctgtatgg cgcgatcgtc gaacagatgg cggagttctc gcggcgcggg atcgaggccg
     8521 agatcgtccc cggggtctcg tcgctctttg cggcggcggc ggccttaaag acccagctca
     8581 cccttcgcga cgtctccgag agcctgatcg tcacccgccc ggcgggggcg acgcttgcag
     8641 aggaccggat agccgagttc tcccgcctcg ggcagacgat ggtggtcttc ctcgggaccg
     8701 agcggatgga ggaggttctc tcccgggtgg agtgcccgcc ggagaccccg gcggcggtcg
     8761 tctaccacgc ctcgtggccc gatcagaagg tcgtcaccgg gacggtcgct gatattgcgg
     8821 caaaggcccg ggcggccggg atcgagcgga cggcgctgat catcatcggg aaggtggcgg
     8881 atccggcaac gtccgggttc gggaggtcgg ttctctactc atgaccggga cggttgtggt
     8941 agcgctcgag cggttcctcc ccgacgctcg ccggattgcg gatatgctcg gcgccgacac
     9001 tatcccctac ggccccgatg ccttccggga ggcattttgc cgttaccgcc ggatcgtcgc
     9061 cctgatgtcg gcagggatcg ccgttcgcgg tatcgcgccc ctcctcgccg acaagtggcg
     9121 ggacccggcg gtggtggtgg tcggcccgga cctccgttac gccgtcccgg tcgtcggcgg
     9181 ccaccacggc gcaaacgacc ttgcccggga actcgcccgc ctcggtatcg agccggtgat
     9241 cacgaccgcg accgagaccc gcggccggga gtcggtcgaa aggatcgccg cccgatcggg
     9301 gtgcgatatc gtgaaccgcg actccacccg ggcggtgaac gccgcggtgc tcgacgccga
     9361 cgtacccctc tacgcgatcg ccggccccgg catcgtcgtc gcgggtccgg aagtctcgct
     9421 tctcgtccgg aagggcgaat acgtcgtcgg cgtcgggtgc cggaaggatg tcccggcggc
     9481 cgacgtgacc agagccgtcg gcgaggcctt ccgggaggcc ggagttgccc ccgcagaggt
     9541 cctcgtctac gcgacgaccg agaagaagcg cggggaggcc ggtctccttg ccgccgtcgc
     9601 ggacctcggc ggcaacctcg tcttcttaga cgacgaaacg ttgaacgcgg aggcggctcc
     9661 gtcaccctcg cgcgcctccc tgatcgggct tgccggtgtc gcggaacctg cggcgctcgc
     9721 ggtctcgaaa cgaaaagaac tggtgtttgc caagcagact tatgggagtg ttaccgttgc
     9781 aatcgcacga taatggagga aaactgtaca tcgtcggcac cgggccgggc gaactgctgc
     9841 agatgacgcc ccgggcgtca aaagccctcg aagaggccga ctgcatcatc ggcaacgggt
     9901 tctacctcga tctcctcggg ccgatgctcg ccggaaaaga ggttatccgg agcggcatgg
     9961 gccgcgaggt cgaccgggct tcgcgcgcgc tcgcccttgc cggggagcgg gtcgtcgcga
    10021 tggtgagcgg cggcgatgcc ggtgtctacg ggatggcgag catcgtgctc gaggtggcgg
    10081 agcggtccgg cacaaaagtc gcagtcgagg tcatccccgg tatcacggcg gcgatatcgg
    10141 cagcggcgct tcttggttcg ccgctctcgg gagactacgt cacgatgagc ctctcggatc
    10201 tcctgacgcc gggagaggag atcgaacgaa gactcgatct tgcgtttcag atgcgggtgc
    10261 cggtcgtcct ctacaaccct cgttcccggg ggaggccgca caacctggat gcagcggtcg
    10321 cgatcgcccg ccggcacctt tccgacgcga cacccgtcgg ggtcgtccgg aatgcctacc
    10381 gggagggaga agagcggttg atcacgacgc ttggcgagtt cgaagaccac ttcgccttcg
    10441 tggacatgca ctcgatcgtc tttatcggcg gcgaggagac acggatctgg agggatgaga
    10501 cggatgagag aggaatcatc acgccccggg gataccaccg gaagtacgta tactgacctt
    10561 gcggcggtca cccccgaggc ctacgcgata gcacgcacga gtcggaacct tgcccgggag
    10621 cgggtgggaa acgtcaccct cgaggaccgg gtacggcagc gatgctcggt cgcggtcggc
    10681 gattttgcga tggccgatct catgcggttt tccggcgatc cggtcacggc ggggcttgcc
    10741 gcactcgatc ggcgggtgcc gatcataacc gatatccgga tggtgcaggc cggcatcctg
    10801 aaacgcgggc acgagagtga gatcctctgc gccctcgatt atggggagga tatcgcgcgg
    10861 gagcgcggga ttacccggac gtctgcaggg tttgtcgccc tgcaggaacg ggtggagggt
    10921 gcgatcgtgg tcatcgggaa cgccccgtcg gcgcttctcg tcgtctgcga tatggtgcgg
    10981 gccaacatcc ggccggcgct ggccatcggg accccggtgg ggttcgtgaa cgcggcggag
    11041 tcaaaagagg cgctccgtac cctcgacgtg ccgtcggtat ccaatgccgg cacccggggc
    11101 ggcacaccgg tcgcggtggc ggcaatgaac gagatcatca cgatctttgc cgagcgtgca
    11161 ggctatgaag gatccggtta ctgatttcga gtatcccggc gcgtgggtgg cggcatgccg
    11221 gtcgcccgac ctcctccccg acgtccggcg gggccttgct gtcctgacgg cgtcgggaac
    11281 agtgctccgc cgggggttca cgaccgggac gacggcggcc gcggcctgca aggcggcggt
    11341 cctctcgctt gcggaggatg aggtgaaggg cgtgggcatc accctcccct gcggcatcgc
    11401 ggtcagggtc ccggtcgacg cctatcgcgg gaccgcgtcg tgctggaagg atgccggcga
    11461 ctaccctgcg gacgtgacgg cggggcttga gttcgtcgcg accgcggttc cctcgctctc
    11521 ggggtcggtc cagttcgtcc ccggcgaggg tatcgggagt ttcgcccgcg acaccccccg
    11581 tcatcgtcag gggacgccgg ccatcagcgc cccggcgctc gactgcatcc ggcggtcgat
    11641 cgacgaggcg gtggaggaag cggggctcca cggggcgacg gtcatcctca cgattccgcg
    11701 aggggcggag gtggcgcaga agacgctgaa cccgaaggtg ggggtgcagg ccggcatatc
    11761 ggtcctcggg acgaccgggc tcgtggagcc ctgggatgac cacctcgcgg agggggtgct
    11821 cgaccgcatt gcccgggctc ttcccggggc ggtcgtcctg acgaccggcc ggctcggtct
    11881 ccgctactcc cggctcctct tcccggagca cgaggcgatc ctcgtcggga gcaacctttc
    11941 agaggcgctt cgggcagtcg acggggacgc ggtgctctgc gggctcccgg gcctgatcct
    12001 gaagttcatg aacccggccg tcctcgacgg gaccgggtgc gcgacggtcg aggaactctc
    12061 gggctccccg ctctgggagg aggtggcccg ccgggaggtt cgtgcgttct ccgttcgcta
    12121 cccccgcgtc cgtgtcgtga tcgtcgaccg cggcggccgg gtcatagcgg agtcgccgtg
    12181 aagatcgtcg gcgtgggctg cggcccgggg atgctgaccg cggaggcggc ccggactctc
    12241 cggggggctg ccctcatcta cggttctgcc cgggcgatcg cgcttgcccg ggacgttttc
    12301 ccgccggggt gcgaggtcca cgaggtcgcg gattatcgag atctccggtc tctcccggac
    12361 cgtgcggtcg tcctctcgac gggcgacccg atgcttgcag gcctcggcta cctcccgggc
    12421 gacgtgatcc ccgggatatc ctctctccag gtcgccttcg cccgcctgaa gatcccgctg
    12481 acgaaggcgg cggtcgttac agcccacggg aaagaccatg cccgggccgt ggccgatgcc
    12541 cgggaggagg tcctccgggg ccgggtggtc tttctcatcg ccgacccggc cttcgacgtt
    12601 caggcgttcg cggccgccct cccgccggag accggggttg ccgtctgcga agacctcggc
    12661 tacccggcag aacggatcgc cgtcgggacg ggggcggaac ccccggcgcc acgggggggg
    12721 ctttttgtgg tggtggcggg ggagttttaa ggcagttgcc ggcaccgtcg cctacaacgc
    12781 gggagcccgg aggttcctca cgatgggccg gtccacctcc ctcttccgga actcgtagcc
    12841 cgaccggagg agatcgagga atatggcctg gtacgccccc cgctcacaca gctcgaggtg
    12901 gtagcgatgc accgtcgact tcgtcccgta ctttgccggt acgtcgctcc aggtacaccc
    12961 ggtggagagg acatacagga tcccgttgaa gaggtgtcgg ggatcccggc gcggccggcc
    13021 gacgtggggt ttccggggcg gaaggtgcct ccggatgatc tcccaggttt cgtcgtcgat
    13081 ctcccggaat gtcatgcgag cccgccatat cacccggggt tcttatagtt gtgggatgat
    13141 tccgggcgtt tttgcgcccc gaaaagatca cgtatccggg gtgttatcgc tctccgggag
    13201 cggacgggtc gtagttcgtg acgatgatct cgcggatctc gccccgtccg tctccgttgc
    13261 agttgatcat cctccgtgcc ggaacccggt cgatgcggta tccggcatag aggtcgtcaa
    13321 agaattcgtc gcacgggtcg acgttctttg gatccgagtt gctcagcatc agcctggcgc
    13381 cccgggcgtc gcatcgcgcg taatatgccg caaggcgtct ctgctcctcg tcggtaaacc
    13441 cttcttttgc atactgcgtg aatgttgacg tccggttcag gggccggtac gggggatcga
    13501 gatagacgaa ggtatctttc gatacataca gttccgactg tgcgaagtcg tcgtgatgga
    13561 tggtggtgtt ccggagcagt tcggcatcgg cccggagcac atcctcgttc aggatcgtcg
    13621 ggtttcggca tctgccgaac gggacgttga acccgccctg caagttgacc cggtagagac
    13681 cgttgaaaca cgtcctgttg aggaagatga gttgagccgc ccgctctatc cagccctcgc
    13741 cgtactgccc gaaatcgatg gtctctttcg cgcggttgaa cgtatcccgc atggcgtagt
    13801 agaaatcctt ccggccgaga tcatcctttg tgaggtagtc gtccgatatg ccggcaagat
    13861 agtcgatgag agcgcagacg tcgctcttca ccacgctgta ggtgaggatc agttcttcgt
    13921 tgatgtcgaa tatgtggcac tctctgaacc tgaaggtgct gttgaagtgg aagtataccg
    13981 ctccaccacc tacgaagggt tcgacaaaga ccgggagggt tccctcgctg agctcccggg
    14041 gaattctcct ggtgaacgca tcgagcagtt gcgcctttcc gcctgcccac ttgaggaacg
    14101 gtcgtgcagt gtatgttgcc atcgctctct tccatccggc gttatttcct gaaatgttgt
    14161 tcccgaaatg ttttagtgtt gccggcctcc ggcggggata tctcccgatc cgggtctttg
    14221 tcggtccggt tccgggtgtc ggcaacctgc ctccccggcg catcgccgga tcttttgccg
    14281 gcagcaagtc ccggcatcca ccggcacggt tgctccggga ctcattcttc tcgcgaacga
    14341 ggggccggcg tcgtcgcccc cctccctgtt ctccggaccc cctctctctt cagcatcttc
    14401 tcgatggccc accgtccccg gcagagttcc tccgccaggg tatcgacggc cttcttcttc
    14461 gtcgcgccgc cctcaataag ccgggcgaac cgggagacca gggcctcgat ctcatcctcc
    14521 gtccagggcc ggccccggtt tttcggtccc gaggccagcg gagttctcaa cgacggcagc
    14581 agacatccct gcccgatgcc ccgcaactct ttgactttca ggtcgaagac aggtcctgag
    14641 agctcaaacc cggtcgcccg acgattgagg ccgacggccg ccctggcggt cgagaatccg
    14701 ccaaggaaca tatcgcagac gaggtcgccc tcgttgctgc tgtactgcag catctttacg
    14761 agcagttccg ccggcagttc gttcttgttc ttcgccttcc cgggtttgta ctcgcggttg
    14821 acgacccaga cgtcttcacg gtcgcggtag ttgggcgacc cgttatccgg tgccttctcc
    14881 ccggttccat accgcgactc aaggttgaac gtcctcttgc cgcccggctt ttcgtagaag
    14941 aggatgtggt agtgggagga gacgtacttc cggctggtgt agacgccgaa gttgtacttc
    15001 catatgatgt ggttgacctc gcgaagctct gtctgccgga gggcgtggag tatgtggtag
    15061 aggttcgtgt agcccgatac gatgtagatc gatcctccgg gccggagtat ccgtcccgcc
    15121 tcccggatcc actgccggct gaactccccg tacccggctg cgggaacctc gacgtagccc
    15181 tcgacgacga acgcctcgtc ccggttgtag tgccggtgca gccggtcgcc gtcgatgccg
    15241 tacggggggt cggtgacgat caggtcgatc gtaccgtcgg ggatgtgctc ccgcgccccg
    15301 cggatgcagt cgccgttgta gaagatgttg ccgttgatct cttcggacct catacgttct
    15361 ccgcccgaat cctgcagata tcgggctgtg gtgtacacca gatgggttgc gcgagaataa
    15421 gattgtggct tttccaggtc tcacggcggt acgtgcaccg gtcacggggc ccggggcgat
    15481 ggttgagtgc gaggagtggg atcgggcgat ctctgccgac tcccggcggg accgaccagc
    15541 agaggagagg tgccggcccg gcactccccg ttgcggtcgg aagccccgct cccgccccct
    15601 tcacctgccg gaggtagtat tatattagac gggtgaatcc agctaagagg gagggacgaa
    15661 gcgccccggg attttagatg acggcaggtt ccgatgtgac ggagacttca tcccgcatgg
    15721 gagcggaaga gagggagcag aagacctccg cgatggagga ggggccagtt cacgagtgta
    15781 ttgccgcgat cctggctgaa aacgaggcgc tccgacggga aaacgctctc ctgcaatcca
    15841 tgaaaaatgc cctggaggag ggcgagatgc gataccggcg gctttttgag gatgccctta
    15901 ccggggactt catcacagcc ccggatggcc ggataatcgc ctgtaacccg gcgtttgcca
    15961 gaatgttcgg gttttcctcc gtagaggggg cactctgcac caatgtccgg gatctctatg
    16021 aagacccggg ggaccgggac cgccttctcg cgcagctccg gagaggaggc agggtggtga
    16081 acgagggccg gacgcggaag cgccgggacg gcacccggat ccatgtcgtg gagaacgtgg
    16141 tcgggcgtct tggtgcggac ggcgaactca tcgagatcca gggttacacc tatgatgact
    16201 ctgagcgcaa acgggtggaa gatgccctcc gggagagcga agagcggttc cgggcagtcc
    16261 tggaaaactc cctggattcg gcctaccggc gcaacctgca ggccgactgc tacgactaca
    16321 tgagcccggc gatcgaggag attcttggct ttacgccgga ggagatggcc gttatgagcc
    16381 tcgaggaggt catggaccgc attcatcccg atgatcgttc gctggttaaa gcggaactca
    16441 acaccgctgc tgcttcgggc agagggctcc tcgtgtaccg gttcctggcc aaagggggtg
    16501 aataccgctg gctcgaagat cacttcacgg tgatatccga ttcggctggc cggccgctct
    16561 tccgcggcgg catcgtccgt gatatcaccg ggcgcaggcg ggctgaagag gctatcgcgc
    16621 ggagtgccgc ggagcttgcc cgggttcacc gggatctcga gtccgctcac cgggaggcga
    16681 acctctacct cgacatcctg acgcacgata tcgggaacac cgagaacgtc tcgaaccttt
    16741 atgccgaact gctcgccggt tccgtggaag gggaggctgc caggtatgcg gcgaacctga
    16801 aacggagcat agaaaagagc atcgagatcc tcagaactgt ctcgaagatc cggcgaattc
    16861 acgcaggacc acccggtctc cggccgatcg atctcgatgc ggtcgtcagg gcggagatcg
    16921 cccactttcc cggtatccct atcaggtacg agggcgctcc ccgaacggtc cttgcggacg
    16981 acctcctctg cgaagtcttc acgaacctga tcggcaacgc cgtaaagcac gggggccccg
    17041 gtgtcgccgt gatcatccgt gtcgaggcgg aggaagaagg tcttctccgg gtgacggtcg
    17101 cggataccgg ccgaggtgtc ccggaagagc ggaaagagga gatcttccac tgctacgaga
    17161 ggaagcagcg gggcgtgggc gaggggctcg gtctctacct cgtgcggatt ctcatcgacc
    17221 gctacggcgg caggatccgg gtcgaagatc gcataccggg ctatcccggg gagggagcag
    17281 cgttctcctt cctcctccgg gaggcggagc ggtcctgaac gggaccggcc gaccttccgg
    17341 gggttgcaat ctccggtctg cccgcggtga gcagagcgct caatccagaa tgtatatcat
    17401 cccctctcgg cgaacaatca tatgatgagt gagcaggagc agcgccggat ccccgtccgg
    17461 accaagatcc ttcttgtatt cctgggcctc tccatggccg ctctcctcct tgcgggttct
    17521 cttgccttcg tgcagatgga cgatgtcagc cggtacgccc ttgcgcggag caccgatctc
    17581 ggggcgcgtg cggtgaacga cagcaccgcc gcgcttgagc gcaacgccga agagtcgctt
    17641 ctccggcttg cgcaggatca ggcctacatc agcaccatcc tctttgaaca ggtgaacggg
    17701 gatctcgagg tcatggggcg ctacgccgca accgtcatgg caaacccgtc catggtccgg
    17761 ccgcgccact tctacctgca ggacgaagaa ccggaggatc ggcgtgcaac ctccgtcctc
    17821 ttcctctcgc ccggggtcga cgggggccgt cttcgggatg agcgcaacgc cgcagggatg
    17881 atggacgaca tattcatccc cgtctccgca acgaaccggc accttgccgg cgtctacgtc
    17941 ggtaccggtt ccgggatggc gatgatctac ccctggacga ccggacttga tgctacgtgc
    18001 gatccccgtc tcaggacctg gttcgtacag gcggtcgaga cgggagagac ggtctggtcc
    18061 gagccgtacg tcgacctcat cgggcacggc ctgatggtga cctgttcgca accggtctac
    18121 gggcccgaga gagactgggt ctgggtggtc ggtgcggacg tcacggtcga gatgatcgac
    18181 cggcagatcg tcggcaccca ggtgggcgac cggggttatg ccatgctgat cgaccagcac
    18241 ggaaatgtca tcagccggcc cggcctcacc tcgggggata tgcggtggga cgagtcgttc
    18301 atcaccgaga acctgctcgc gagcgagaac ccggatatcg tcgcggttgc taaaaagatg
    18361 gttgcggggg agacggggat cgcccgggtc gggtttgagg acggcgaacg gtttgtcgcg
    18421 tatgctcccg tccggggcgt gaactggagc gtcggggtgg tgatgccggt cgacgaggtg
    18481 cttgcgccga taacgacgac ccgggcaagt atcctctcgg cgtcggagga caccgccctg
    18541 cacatcggcg cccagcagga ccggatgaaa gccgtcttta ccggggcgtt tgtcgtcctg
    18601 ctcgtcgtcg tcgccctcct gacccttgcc gtcacccggt acttcacccg gcccatcgaa
    18661 gagctgcaga gggggtcgga ggccataggg cggggcgatc tcgagcaccg ggtgaatgtg
    18721 gcgaccgggg acgagttcga agaccttgcc cgctcgttca accgaatggc ggccgatctt
    18781 cgcgggcata tcgaggatct ccggcggacg actgcggaga aggagcggat agaaaaggag
    18841 cttgagatcg caaaggagat ccagcagagc atcctcccgg agtccgcgcc ggtcctcccc
    18901 gggttcgata tcgcgggctt caacctgccc gcccgggagg tgggcgggga cttctacgac
    18961 tacatccctg tcggcgaggg tcgctggggt gtcgagatcg ccgacgtctc ggggaagggc
    19021 gttccggcgg ccctcttcat ggcgctctcc cgcaccctcg tccgggcgag cgcctcggag
    19081 aacccggatc ccgccgggtc gatccttgaa gccaaccgct acatatgcat ggactcaaag
    19141 acctgcatgt tcgtcacgct cttctacggc atactcgata cccggaaggg aaccttcacc
    19201 tacgtgaacg caggccacaa cccgccgctc ctcttccggg cagggtcgag cacggcggag
    19261 ctcctccggg ggaaggggat tgctctcggt atattcgacg atatcgagct cgaactcgtg
    19321 gagctccggt tgaatgccgg cgatacggtg gtcttctaca ccgacggggt gaccgaggcc
    19381 acgaacgagc gcgacgaaga gtacgggatg gagcagctta cggcgctcat accggggctg
    19441 ctcgatcttg gagcacgggg gatgatcgat gcgatcgtgg cggatgtcac cgcctttgcc
    19501 ggggaccgcc cgcagtttga cgatatcacg ctcgtggtgc tgaaggtcgg gtgacgaccg
    19561 gggatcgcac gccggattgc cccccgggag cgggcgggcg aggggttctc cggccctgtc
    19621 tgaaccctcg tttctgcgcg gcggatcgtt tcgccctgtt tgtgggtttt ggtgcgggag
    19681 ggagaccgcc gggtccggtc cc
//
LOCUS       DAOSEE010000024        19280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1180_length_19280_cov_247.450351, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000024 DAOSEE010000000
VERSION     DAOSEE010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 19280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19280
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1180_length_19280_cov_247.450351"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PLI76_03230"
     CDS             complement(<1..469)
                     /locus_tag="PLI76_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI61073.1"
                     /translation="MEQEEGRPYSRRLILALITVATFLNPFTGTAINLALPSIGAEFA
                     ADAATLAWVSSAYLLASVIFLLPGGRLGDSRGRVTIFMAGTIIYAAGALLTILTPTIG
                     MLLLFRFLQGIGGAMIYANSVALITYLYPPGERGYAIGLNTTAVYAGLSLGPFL"
     gene            610..1815
                     /locus_tag="PLI76_03235"
     CDS             610..1815
                     /locus_tag="PLI76_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI61074.1"
                     /translation="MPDSSPEVRGLSVRLILLLGTASLFGSLVSNGARSVTGPFLLAL
                     GGSAVVVGLIVGFGEFAGYALRAITGGYVDRDHGYWRVVMAGYGLLAAIPLLAAIGRW
                     ETAAVLIITERVGKAVRTPARDTILSHATTNVGRGWGFGIHKALDQVGAVIGPLVMVA
                     ALTLTGGYAEGFLLLGLPLAGVALTLSFAQSTVPRPGSLDTGERDGKTIGSPRVLSYA
                     VFIFLGMAGFVNFPILSFHLKAQAIVPDAAIPLFYASAMVVSVVVALLAGRAFDRIGT
                     RILLTIPVISTLTVLLAFSRDPGVALAGSLAWGVGIGIFETAFRASIAGLATAGRRGR
                     AYGTMSAVFGTAWFVGSAVMGILYDLSIGYIVVYVVIVEIAAVVACLWMYRSRIAVRL
                     EEGIGNLMP"
     gene            complement(1855..2193)
                     /locus_tag="PLI76_03240"
     CDS             complement(1855..2193)
                     /locus_tag="PLI76_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5518 domain-containing protein"
                     /protein_id="HOI61075.1"
                     /translation="MADNFWVGVIVGWLVGLILGFFLPVVGPLVGGFVAGWIVRGGIG
                     NGAKAGLLAGIIGAIIISILILVGGTVLLGAFGLVAGVGTSIALVVAAFVYQGILSLI
                     GGAIAGAIRR"
     gene            complement(2254..3198)
                     /gene="arcC"
                     /locus_tag="PLI76_03245"
     CDS             complement(2254..3198)
                     /gene="arcC"
                     /locus_tag="PLI76_03245"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOI61076.1"
                     /translation="MTGKTVVVALGGNAIRRHDEAGTAAEQIANVRRASRQIAEIAAD
                     GYAVAVTHGNGPQVGDILVRNELTKDTLPAMPLDVCGAESQGMIGYLLQQAMQEALRA
                     AGLDRPVATVLTQTVVDGSDPAFERPEKPIGPFYTADQAERLRKEKGWRMVQIPGRGY
                     RRVVPSPRPIALVEGEVILRLFSAGVIVIAAGGGGVPVIAGDGGAFCGVEGVVDKDRT
                     AALLAGIVGADDLLILTDVECVSLNYGGPNREDIHDMTAREAREHLAAGHFPPGSMGP
                     KVEAAIRFLEAGGKRATIASLDSAAAALAGRSGTRIRR"
     gene            complement(3203..4501)
                     /locus_tag="PLI76_03250"
     CDS             complement(3203..4501)
                     /locus_tag="PLI76_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HOI61077.1"
                     /translation="MPAGVRAEWEPLRKVAVHRPGIEMFFGLLEPYAALYERAFSRYE
                     ARREHDALVRALHEEFGVRVLRLKETILDAADSDPAVRRRLVDWAYETVTLRGGRREV
                     AEARRSMEQNADALDSLHFFTLLLLNPVIEVGRGDTGDGVDVRVPGQEPLANLYFMRD
                     QQAVTARGLVVGRPAKRQRLREPGITRFLWDILGIPVAGTVEGPGTFEGGDFMPMGTF
                     ALVGTGDRTNPAGVSQFLDFDPGFDEIGVVHQPGHPLIPGDRPDPMVDMHLDTYFNVA
                     GSGVVIGSEVLLRRARVDIYHRTGEGYVRSGETVSLDDYIRSKGFAVIDLSILEQLSY
                     AANILCIRDGSILAVEGERVMQTVLLNLERKAARDPHRYGRLLCHAEEDYRRIRAAGR
                     FFPHRKEFSRHGIDLRPLHLENLTGGYGGPHCMTCVLERG"
     gene            4828..5028
                     /locus_tag="PLI76_03255"
     CDS             4828..5028
                     /locus_tag="PLI76_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61078.1"
                     /translation="MRIRRYKPPISVSTQPFRTVRRLQQNPTYTPLMTGPVVNAIVLA
                     ALSATSAMSGLTLGVCMRLVGL"
     gene            complement(5055..5549)
                     /locus_tag="PLI76_03260"
     CDS             complement(5055..5549)
                     /locus_tag="PLI76_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61079.1"
                     /translation="MTPAARAFLCLCLLLFVLAPAADGLVITNTDLTPAEVNLLFAMR
                     EYTLTYNEYAGRLPGWVTGPFENEKVNLYVEFPDAVLVIGITFRDRKIVSFDAKGCPD
                     PIVTVTTDKATVEEVMASGRPFDTFRAAMRNGTVTVEGNTLLGMLRSGMVRFGLRVMD
                     IVGL"
     assembly_gap    5841..5940
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5968..6918
                     /locus_tag="PLI76_03265"
     CDS             5968..6918
                     /locus_tag="PLI76_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOI61080.1"
                     /translation="MSGDDIIEVQDLEHAFNGFQAVRGISFTVRRGEIFSFLGPNGAG
                     KSTTINILTTLLPLQQGTVRVAGHDVAREPGRVREAIGIVFQDDVLDRDLTVWETMEF
                     HGRLYSIPRDERKARIDEMLAVVELDAKRNERTKNLSGGMKRRLQIARGLLTQPEVLF
                     LDEPTQGLDTQTRMRMWDYIRGVNAAGTTVFLTTHYMEEADMLSDRISIIDHGRIIVS
                     GTPEELKNRLGEDVVYLETGDDAKVRDALQGIDEIRSITESPRGLSITISADGSHCLP
                     RIVERVHDAGIAITSVNLKKPTMDDVFVHYTGRELRDTGA"
     gene            6926..7762
                     /locus_tag="PLI76_03270"
     CDS             6926..7762
                     /locus_tag="PLI76_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI61081.1"
                     /translation="MAWEFLTVYWREMIRFTRFRTQLLSSLLQPALWMAFFGVAMSSN
                     FNRFASSVPPPAGVVPVDYLTFMAAGVIAMTTLFTSLFGGISLLFDKNWGLMREMLAS
                     PMPRTHIMLGISLSGMTKSFIQVAIIMAFGLFLGVQFFPGYSPARVVVSILGIFLFVG
                     VFSLGFLLFSSTISMRLESPEGLQGIITLLTLPLFFVSNALYPIQAFPPLLQDLSIYN
                     PLTHLVTGVRYFALGNEFFVVGTNYSYTPADILLSFAYLVAFAAVMYAVAHRTFQKAV
                     VT"
     gene            7862..8824
                     /locus_tag="PLI76_03275"
     CDS             7862..8824
                     /locus_tag="PLI76_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOI61082.1"
                     /translation="MAHLLPFTVAGVACALPLADVQQVTGMVELRPMTGGRDVGAVNL
                     HGRIIPVYSLRRRLGLPDRPPHPDDALIIARAGRDCAALWVDAVERVRVSTTPLPREG
                     EPSPLPGVLLTEEGMALIYDLPLFLAAEESASLPRPPDTDGDEPPREPGRARTILADR
                     ARALMQRKEEPRETPFPELLTFRLAGREYAVGMQYIREVFILREITPVPGVPDFIAGI
                     GAVRGEIISVVDLCALFSIPKHGLTDLNRVIVLSDGAVTFGILADYITDIGTRPTDHL
                     APVDPGMTPIDRRYLVGVTDESVIVLDAAALLASPGMVIDEARK"
     gene            8954..11335
                     /locus_tag="PLI76_03280"
     CDS             8954..11335
                     /locus_tag="PLI76_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cache 3/Cache 2 fusion domain-containing
                     protein"
                     /protein_id="HOI61083.1"
                     /translation="MFTFFSDIKVGTKILVICLFLAIIPTLMLGIVAYTSSSGVINEQ
                     IETLLETQVHDARGWTNDVYKLTRNKVNSDLNVLRENFYARGTPEVINGKLTLVNAGG
                     EPYVINDNFEIVDQVQSLVGGAATVFQVYDNNAIRISTNVIGTDGSRAVGTELTQNVY
                     DVVVNQGETYYGSRDLFGKRYVTAYEPIKDSSGKIVGVLFVGTEEEETLDVVKASLKD
                     TVIGRNGYMFVIDSNGQVLVHPSLEGENWSSEAFVQEILQKKVGIIRHQVDGTDMIDA
                     YTYYEPLDWHIVSRAELSDFTGPIDTIRSTIVIVVLASIAIGVAIAILFGRSISDPLQ
                     QVVLMLKELRNGHLSARLNIRRQDEIGVMARTMDEFAGDLQTNVVGNLKKIALGEYVQ
                     EFPVADEGDEIRPALALMVQSLDHLHKETLKLTDAARAGDLSIRGDEKAFRGGYRMII
                     AGFNKTLESITEPVNEAMRLARIYASGDFTARFDEKIPVAGEFVAYRDALNTIGIELS
                     RLMKLISEELYEGVSVLSLASNEILAVTTQLAAASSQTAATVNETSDSVESVRKKTEL
                     VNQKTKGVSEKAMRALEVSKGGQRSVRETLEGINQIQRQMDIIRMNVIRLSEQSQAVG
                     EIIATVTDISEQSNLLAVNASIEAAKAGEFGRGFAVVANEIHNLAEQSKKATSNIRVI
                     LTDIQQGISSTAASTEQGTRSVADATRLTNNAQEAIEVLARSIADTSQEVIEIATSIQ
                     AQASGVDQISSAMENIRDAAQKNLETTHKAEKTAGDLHELGARLKRITQQYRV"
     gene            11347..13620
                     /locus_tag="PLI76_03285"
     CDS             11347..13620
                     /locus_tag="PLI76_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOI61084.1"
                     /translation="MSGTDEQFRARLLDMFREEADEYLEAIAAGLIELEKTGLVPELV
                     ESVYRRIHSLKGASRAVNLREIESICQNLETIFSLVKRGEYTPGVNDFDLFHRTLAVV
                     KALLQEEQPDASPAEIVGALRAIPGKPAGSGPGPALREPHAPEARNGNDPAVTGPDRG
                     TVRIAAHKLDRLTASADGLLTAQISIMQRIRELEEMVARFTLWQRNHSQAFNDLNLLQ
                     RKVFREEKAALPHDLVLPLQRAAEFLEYNREFVTYLQHDLATHLRAMEIDRSALKAST
                     AEISDLIHDAALLPAASMLAPFSAFVREASRAAGKSVELTIEGGEIEVDRRILDALRE
                     PVMHLIRNSIDHGIEEPPVRIAQNKPATGSVRVRIFSRSGSRVGIEVIDDGAGIDSSA
                     IRKTAVESGVITKEEDAALTDSEAIWLIFRSGMTTSRIVTDLSGRGLGLAIVEDTVSR
                     LGGRMTVSSVVGRGTTISFSVPVRMTTLRGLLVRSGRQVYVIPIQQVKQVLRVHPDSL
                     TSSRGRPTIRFSGETIEVIRLTDILGIPPSGPPPADTRPTPLVIIAYGAGQIACTVDE
                     VIRVQEIVVRPLGSQLRSVRRIEGAVILGDGSVALVLDPLELIQDALQADRPAPGPAL
                     PGEAGWRILVVEDSVTSRVLLQTILEEAGHQVETAVDGIDAFARLKQHEFDMVVSDVD
                     MPRMNGFTLTEKIRAEGRQFAGLRVALVTSLDSPEDRERGRAVGADAYVVKSNFNTED
                     FLETVRRLMRRRYGEVR"
     gene            13622..14941
                     /locus_tag="PLI76_03290"
     CDS             13622..14941
                     /locus_tag="PLI76_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HOI61085.1"
                     /translation="MRDTDNRTTMSVLVVEDSRTQAELIRHILEKEGYDVALAADGAR
                     ALQRIADNRPDIVLSDIMMPGMDGYELCRRIKRDFPDIPVILVTNLFDPADVMRGLAS
                     GADSFIVKPVDPRHVRSQIEAVQRAAEGSDPDGTPAGLDVSFSGNTYTITARRRTILN
                     TLLSTYTVAVAKNTELQEAQEQLHSLNDRLQEAVDDLSRSNERLAAENQERRRVEKAL
                     ADANRKLQLMASITRHDLLNQLAALGGYLDLAQMMREREPANAWKQVASAGEIVNRIS
                     NTIRFTAEYQKVGSASPAWQDIRSLVEKSERYVSAETVTLVNAVPAGAEVYADPLIEK
                     VFSNLIENAVRYGKTATTVTFSLRRSGDTCTILCEDDGVGVPYDEKEKIFTYAYGMNT
                     GLGLFLAREILAITGIAIRETGEPGRGARFEILCPVGVIRAPERHTG"
     gene            14984..16384
                     /locus_tag="PLI76_03295"
     CDS             14984..16384
                     /locus_tag="PLI76_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOI61086.1"
                     /translation="MNAGDSLTAYFSGRRNRTATVAGLTVLTLGGSLFGMILGAPANL
                     IPALLLSIPIILASYWYPRRGTLFSACIAAAYTVTAFLFSPPVAHLALSVLPRAALLI
                     LVGGVVAFLSTRLRESEQQMNQIIEFLPDATFAIDLEGRVITWNHAIEDMTGVKKDAM
                     IARGDHEYAIPIYGERRALLADLVLQGDPGSGYPSVRREGDIFIAEARAPHLRGGRGA
                     QIRFAATTLLDHEGRVTGAIESIRDITDEVMMEAALQNTNRQLNTLSGILRTDLSNRL
                     AVLYGHLSVGAMKFDDLEVLSFIDDLKDAANGIQRQIEISREFRNIGTSPPAWIPVQQ
                     IARNAADTLSFAGVSLEIWVERLEVFADPHLGTVFTHLFENSLAPATGATRVVVTYHL
                     RPDGCAIIVEDDGTGIPDAAKEGLFVQKEKGYGRGLFLAHEILAITGIRIRETGRFGA
                     GARFELLVPPEGYRII"
     gene            16395..16853
                     /locus_tag="PLI76_03300"
     CDS             16395..16853
                     /locus_tag="PLI76_03300"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOI61087.1"
                     /translation="MATDIRSGARPDVRELRSAEFSAADEVWREYRGMVGDPGRDRIF
                     AVFLAGKIVSLARCRRHADGLEVDGVFTPEQYRGRGYARMAVAALVVACHNDDLYMYA
                     VENLTGLYAEFRFMSIPERDLPPGVRERYTWAAGNVEGAEVRPMYRRAGL"
     gene            16945..17805
                     /locus_tag="PLI76_03305"
     CDS             16945..17805
                     /locus_tag="PLI76_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOI61088.1"
                     /translation="MHRTISLAFAILIAATTLAGCTGVPGPEETEVFDRTVAGEPESS
                     VVVVNRNGGVNVSAWDEDYVAITAVKRTVYGRGELAKVSIDVTEGDPLRIESVYTGIN
                     VRAGVDYTIRLPPDVVLQRVESSNGGIELTGVRVAGTELATSNGPVRVAGARGGDIRV
                     STSNGGIDLRDVEGYVVGTTSNGGITVEDCGGLIALRTSNGPISADISAVREDVTIST
                     SNGGITLRFAEDLNARVIATTSNGRVTIRDRSLQVSESTGTSVTGLLGDGGPTITATT
                     SNGGIDLSGL"
     gene            17867..18970
                     /locus_tag="PLI76_03310"
     CDS             17867..18970
                     /locus_tag="PLI76_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOI61089.1"
                     /translation="MADVYFANLRARSHHESKIQKIRRLFDAAGFDRVVRPGDLTAVK
                     LHVGERGCDTYINPIFARQVVDKVKERRAHPFITDTGTLYAGSRADAVRHTVTAIEHG
                     FDYAVVGAPVIIADGISGGYWREVAVGGNHFENVRIAGGILDAESMLVLSHVKGHDLA
                     GFGGAIKNLAMGCAPPSGKAEQHAGRPCFETEACKGCGRCVQVCPQAAVTLVEGRARF
                     NPERCVGCGDCMRSCGEGAIEFDWTTEIRPFIERLCEYALGAVRDKPGRVGYINFLLA
                     ITPDCDCVPWSDAAIVPDIGILASTDPVAIDHASFDLVNSERGIARTALGEHTAPGED
                     KFRGIWDYTDGGYQIDYAARIGLGDASYRLIEI"
     gene            complement(19024..>19280)
                     /locus_tag="PLI76_03315"
     CDS             complement(19024..>19280)
                     /locus_tag="PLI76_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOI61090.1"
                     /translation="LWAEWCGPCRMVAPVIEDLARDFDGRVTFGKCNVDQNRAISTQF
                     SITAIPTLLFFANGMLIDRVVGALPKEAVRARVMRAFPTG"
BASE COUNT         3582 a         6222 c         6098 g         3278 t
ORIGIN      
        1 cgaggaacgg cccgagggag agcccggcgt agacggcggt ggtgttgagc ccgatagcgt
       61 agccccgctc gccgggagga tagaggtagg tgatcagggc cacgctgttt gcgtagatca
      121 tggcgccgcc tatcccctgc aggaaccgga agaggaggag catgccgatc gtgggcgtga
      181 ggatggtgag gagggccccg gcggcgtaga tgatggtccc ggccatgaag atggtgaccc
      241 ttccccggga gtcgccaagc ctccctccgg ggaggaggaa gatgaccgac gcaaggaggt
      301 aggcgctcga tacccaggcg agcgtggcgg catcggcagc gaactcggcc ccgatggagg
      361 gcagggcgag gttgatcgcg gtgcccgtga acggattcaa gaacgtggcg accgtgatga
      421 gtgccaggat gagccttctc gagtacggcc gcccctcttc ctgctccatt cgtcgtcacc
      481 tgcgcttccc ggagagccga ggcccgcccg ggtcggtaca ggagtatgtg cggcgagcga
      541 gaataagggc actgatgcct gcccatacac tacaattaat gtgagcggct ccagaagacc
      601 cccgcgcata tgcccgactc ttccccggag gtccgcggtc tctcggtccg gctcatcctg
      661 ctgctcggta cggcaagcct ctttgggagc ctggtctcaa acggcgcccg gtccgtcacc
      721 ggccccttcc tcctcgccct cggggggagc gcagtcgtcg tcggcctgat cgtcgggttc
      781 ggcgagtttg cagggtacgc cctccgggcg atcaccggcg gctacgtcga ccgggaccat
      841 ggctactgga gggtggtgat ggccggctac ggcctccttg ccgccatacc gctccttgcc
      901 gccatcgggc ggtgggagac cgcggccgtc ctcatcatca ctgaacgggt cggaaaagcc
      961 gtcaggacgc cggcacggga cacgatcctc tcccacgcga cgacgaacgt tgggcgggga
     1021 tgggggtttg gaatccacaa ggcgctcgac caggtcggcg cggtgatagg gcccctcgtc
     1081 atggtcgccg ccctcaccct caccggcggt tacgcggagg ggtttctcct gctcggtctt
     1141 cctctcgccg gggttgcact gaccctctcc tttgcgcagt cgacggtacc gaggccggga
     1201 agtctggata ccggcgaaag agacggtaaa accatcggat ctccccgggt cctctcgtat
     1261 gccgtgttca tattcctcgg catggccggg ttcgtgaact tcccgatcct gtcgttccac
     1321 ttaaaggcgc aggcgatcgt ccccgacgcc gccatccccc tcttctacgc atccgcgatg
     1381 gtggtctcgg tcgtcgtggc gctgcttgcc ggccgggcct tcgaccgcat cggcacccgc
     1441 atcctcctca ccatcccggt gatcagcacg ctgacggtcc tccttgcctt ctcacgcgac
     1501 ccgggtgtgg ccctcgcggg atcgcttgcc tggggcgtcg ggatcggtat attcgagacc
     1561 gcttttcggg catcgatcgc cgggttggcg acggctggcc ggagggggag ggcctacggc
     1621 accatgagcg ctgtcttcgg gacggcgtgg tttgtcggga gcgccgtgat gggcattctc
     1681 tacgatctct cgatcgggta tatcgtcgtc tacgtcgtca tcgttgagat tgcagcagtg
     1741 gttgcctgcc tctggatgta ccgctcccgc atcgcggtgc ggttagagga ggggataggg
     1801 aatctcatgc cgtgattccc gcctctcctg ccggggagga tggtcctccc cgcgttaccg
     1861 ccggatcgcg ccggcgatgg cgccgccgat cagggagagg atcccctggt agacgaacgc
     1921 cgcaacaacg agagcaatcg atgtcccgac gccggcaaca agcccgaatg ccccgaggag
     1981 caccgtgccg ccgacgagga tcaggatcga tatgatgatg gcaccgatga tgccggcgag
     2041 cagtcctgct tttgcaccgt ttccaatccc gccccggacg atccagccgg cgacgaaccc
     2101 gccgaccagc gggccgacga ccggcaggaa gaagccgagg atcagcccca cgagccaccc
     2161 gacgatgacc cctacccaga agttatctgc catacccatt caccgccgtg gattccggat
     2221 atatagtata taagagttgg ggtgtggcgc cggtcaccgc cggatccggg ttcccgaccg
     2281 cccggcaagg gcggcagcgg cagaatcgag cgatgcgatg gtggcccgct tccctccggc
     2341 ctcaaggaac cggatcgccg cctcgacctt cgggcccatg ctcccgggcg ggaagtgccc
     2401 tgccgcgagg tgctcccgcg cctcgcgagc cgtcatatcg tggatatcct ctcgattcgg
     2461 ccccccatag tttaaggaga cgcactcgac gtcggtcagg atcaaaagat cgtcggcgcc
     2521 gacgatcccg gcaaggagtg ccgccgtgcg gtctttgtcg accactccct cgacgccgca
     2581 aaaggcgccc ccgtcgcccg ctatcaccgg gacacccccg ccgcccgcgg cgatcacgat
     2641 caccccggcc gagaagagcc ggaggatcac ctctccttcg acgagggcga tgggacgcgg
     2701 cgagggaacc acccgccggt agccccttcc cggtatctgc accatccgcc agcccttctc
     2761 cttccgaagg cgttccgcct gatcggccgt atagaacggg ccgatgggtt tttccggtct
     2821 ttcaaacgcc ggatcgctgc cgtccaccac ggtctgggtg agcaccgtcg cgaccggacg
     2881 gtcgagcccg gctgcccgga gggcctcctg catcgcctgc tggaggaggt agccgatcat
     2941 cccctggctc tccgccccgc agacatcgag cggcatcgcc gggagggtat ccttcgtgag
     3001 ctcgtttcgg acgagtatat cccccacttg cgggccgttt ccgtgggtga cggcgacggc
     3061 gtagccgtcg gctgcaatct ctgctatctg gcgtgatgcc cgccggacgt tcgcaatctg
     3121 ttctgcggcc gtccctgcct cgtcgtgccg ccggatggcg ttcccaccga gcgcgacaac
     3181 aacagtcttt cctgtcattc agttaccccc gctccagcac acaggtcata cagtgcgggc
     3241 cgccgtaacc ccccgtcagg ttctcaaggt ggagcggccg gagatcgatg ccgtgccggg
     3301 agaactcctt cctgtgcggg aagaaccggc cggcggcccg gatccggcga taatcctcct
     3361 cggcatggca gaggagccgc ccgtagcggt ggggatcgcg cgcggctttc cgctccaggt
     3421 tcaggagaac cgtctgcatc acccgctccc cctcgaccgc aaggatgctc ccgtcgcgga
     3481 tgcagagtat gtttgcggca tacgagagct gctcgagtat cgagaggtcg atcaccgcaa
     3541 accctttcga acggatgtag tcgtcgaggc tcacggtctc gcccgaccgc acgtaaccct
     3601 cgcccgtcct gtggtagata tcgaccctgg cccgccggag gaggacttcc gagccgatca
     3661 cgacaccgct gcccgcgacg ttgaagtagg tatcaaggtg catgtcgacc atggggtcgg
     3721 gccggtcgcc cggtatgaga gggtggcccg gctggtggac gacgccgatc tcgtcaaacc
     3781 ccgggtcgaa gtcgaggaac tggctgaccc cggccgggtt cgtccggtct ccggtgccga
     3841 cgagggcgaa cgttcccatc ggcatgaagt cgccgccctc aaacgtcccg ggcccctcaa
     3901 ccgttccggc gaccgggatg ccgagtatat cccagaggaa ccgggtgatc ccgggctccc
     3961 gcagccgctg ccgttttgcc ggccggccga cgacgaggcc gcgggcggtc accgcctgct
     4021 ggtcgcgcat gaagtagagg tttgcgagtg gctcctgccc cggcacccgg acgtccaccc
     4081 cgtcgccggt atccccccgc cccacctcga tgaccgggtt taagaggagg agggtgaaga
     4141 aatgcaacga gtcgagagcg tcggcgttct gctccatgct ccggcgcgcc tcggctacct
     4201 cgcgccggcc gccccggagg gttaccgtct cgtacgccca atcgacgagt cgccggcgca
     4261 ctgccgggtc gctgtcggcg gcatcgagga tcgtctcctt gagccgcagc acccggacac
     4321 cgaactcctc gtggagagca cgcacaaggg catcatgctc ccggcgcgcc tcatagcggc
     4381 tgaacgcccg ttcgtagagc gccgcatacg gctccagcag cccgaagaac atctcgatcc
     4441 ccggccggtg aacggccacc ttccggagcg gctcccactc cgcccgcact cccgccggca
     4501 tcgcgttcac cgcctccacc gggggcccat acagggattc gcgatcatca cccgggcgga
     4561 ggccgccgcg gcggatacct ggctcaccca cccatcagcg gtgaaataag ggccagaaga
     4621 gagaagagaa acgtgtttgc cgtagaaaag gagttctgta taccgatcca tctgtaggga
     4681 ttctctcgcc ggagatcgga taaatctgtc gtgacagggc tcttccaccc gcccgggtat
     4741 atccggcggg cggaaccgca acgattatct tttcggggaa acaaaggact cacaaggtat
     4801 attcacacga taccaggagg tgtttccatg aggatacgac gctacaaacc ccccatatcg
     4861 gtgagcacgc aaccgttccg gacagtgcgg cggctacaac agaacccgac ctacaccccg
     4921 ctcatgaccg gcccggtggt gaacgccatc gtgctcgccg ccctctcggc gacatcggcc
     4981 atgtcagggc tgactcttgg ggtctgcatg cgccttgtgg gcctgtgaga cccgccgccg
     5041 gctctttttt cccctcacaa cccgacgata tccatcaccc ggagcccgaa ccggaccata
     5101 cccgaccgca gcataccgag cagggtgttc ccctcgaccg tgacggtgcc gtttcgcatc
     5161 gccgcccgga acgtgtcaaa cggccggccg gaggccatga cctcctccac cgtcgcctta
     5221 tcggtcgtca ccgtgacgat cgggtcggga cagcctttcg cgtcgaacga gacgatcttt
     5281 ctgtcgcgaa aggtgatccc gatgaccagg acggcgtcgg ggaactcgac gtagaggttg
     5341 accttctcgt tctcaaacgg cccggtcacc catcccggga gccgaccggc atactcgttg
     5401 taggtcaggg tgtactcccg catggcaaag aggaggttca cttccgccgg cgtcaggtcg
     5461 gtgttggtga tgacgagccc gtctgccgcc ggcgcaagca cgaagaggag gaggcagagg
     5521 cagaggaaag cccgggcagc cggggtcact gtcgttctcc cgcccgctcc tcgaactccc
     5581 gcacgccggt gctccgccgg gaccgggagc aggcatcgcc ggcaggggtg ggcaaccagt
     5641 agccgcacat atcttcctga agagaaatgg gggagtttgg tatttgagaa tacctatccg
     5701 gaccggtctg tgggcagttg ccaatgcggt tttcccgccc gggatcgcgc cggggttcgc
     5761 ggtggggaga gggagatcgc atggtgccgg ggaagagaaa aggagatatg agagcaccgt
     5821 acatggaggt gtgggcgcgg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 ggaggtgtgg gcgcgggcgg tgagcgattg agcggggatg atatcatcga ggtgcaggac
     6001 ctcgaacatg cgttcaacgg ctttcaggcg gtacgcggca tcagtttcac cgtccggaga
     6061 ggcgagatct tctcgttcct cggcccgaac ggcgcgggga agagcaccac catcaacata
     6121 ctgacaacgc tcctccccct ccagcagggc acggtgcggg tggccggcca cgacgtcgcc
     6181 cgggagcccg ggcgggtcag ggaggcgatc gggatcgtct ttcaggacga tgtcctcgac
     6241 cgcgacctta ccgtctggga gacgatggag tttcacgggc gactctactc gatcccccgc
     6301 gacgagcgca aagcacggat agacgagatg ctcgcggtcg tcgagctcga cgcgaaacgc
     6361 aacgagcgga cgaaaaacct ctccggcggt atgaaacggc gcctccagat cgcgcggggt
     6421 ctcctgaccc aaccggaggt cctcttcctc gacgaaccga cgcaggggct cgacacccag
     6481 acgcggatgc ggatgtggga ctacatcaga ggggtgaacg cggccgggac gacggtcttc
     6541 ctgacgacgc actacatgga ggaggccgat atgctctcgg accggatcag catcatcgat
     6601 cacggcagga tcatcgtctc cggcacccct gaagaactca agaacaggct cggcgaggac
     6661 gtcgtttacc tcgagaccgg cgacgatgca aaagttcggg atgccctcca gggaatcgat
     6721 gagatccggt cgatcacgga gtcgccccgc ggcctctcga tcacgatatc ggccgacggg
     6781 agccactgtc tcccgcggat cgtcgaacgg gtgcacgatg ccgggatcgc tatcacgagc
     6841 gtgaacctca agaaaccgac gatggacgac gtcttcgtcc actacaccgg cagggaactg
     6901 cgggatacgg gggcgtgaac agggaatggc ctgggagttt ctcaccgtct actggcggga
     6961 gatgatccgg ttcacccggt tccggacgca gctcctctcg tcgctcctgc agccggcgct
     7021 ctggatggcg ttcttcggtg ttgcgatgtc ctcgaacttc aaccggttcg catcatccgt
     7081 cccgcccccg gcgggcgtcg tcccggtcga ctacctcacc ttcatggccg ccggggtgat
     7141 tgcgatgacg accctcttta cgagcctttt tggagggatc agcctcctct tcgataagaa
     7201 ctgggggctg atgcgggaga tgctggcaag ccccatgccc cggacccaca tcatgctcgg
     7261 gatcagcctc tcgggtatga cgaagtcgtt catccaggtc gccatcatca tggcgttcgg
     7321 gctcttcctt ggggtgcagt tcttcccggg atactcgccc gcccgggtcg tcgtctcgat
     7381 cctcgggatc tttctcttcg tcggcgtctt ctcgctcggg ttcctcctct tctcttcgac
     7441 catatcgatg cgcctcgaga gccccgaagg attgcagggg atcatcacgc tcctcaccct
     7501 gccgctcttc ttcgtctcga acgccctcta cccgatccag gcattcccgc cgctcctcca
     7561 ggatctctcg atctacaacc ccctgaccca cctggtcacc ggggtccgct acttcgccct
     7621 cgggaacgag ttcttcgtcg tcgggacgaa ctactcatac actccggccg atatcctcct
     7681 ctcgtttgcc tacctcgtcg cttttgcagc ggtgatgtac gccgtcgccc accggacgtt
     7741 tcagaaggcc gtggtgacgt gaacacccgt aagaggagag actcatcccg acgatcatgg
     7801 gaatatcgga gatctgcccg taacctttta tccgccccgg accaaccttc gagtatccac
     7861 gatggcccac cttctcccct tcaccgttgc gggggtggcc tgcgccctcc ctctcgccga
     7921 tgttcagcag gttaccggta tggtggagct ccggccgatg accggtggaa gagatgtggg
     7981 ggccgtgaac ctgcacggca ggatcatccc cgtctactcg ctccggaggc gcctcgggct
     8041 ccctgaccgc ccgccgcacc ccgacgatgc cctgatcatc gctcgcgcgg gccgtgactg
     8101 cgcggccctc tgggtggacg ccgtggagag ggtgcgggtg agcacgaccc ccctcccgcg
     8161 agagggagaa ccgtcccctc tccccggcgt gctgctgacc gaagaaggga tggccctcat
     8221 atacgacctc cctctcttcc ttgccgcgga agagagcgca tccctccccc gccccccgga
     8281 tacggatgga gacgagccgc cccgcgagcc cgggagggcg agaacgatcc tcgccgaccg
     8341 ggccagggca ctcatgcagc gaaaggaaga gccgcgtgag acgccgttcc cggagctcct
     8401 cacgttccgt ctcgccggtc gcgagtacgc cgtcgggatg cagtatatcc gcgaggtctt
     8461 catcctccgc gagatcactc cggtccccgg ggtgcccgat ttcatagccg gtatcggtgc
     8521 cgtcagggga gagatcatat ccgtcgtcga tctctgcgca ctcttctcga tcccgaaaca
     8581 cgggttgacg gacctcaacc gggttatcgt cctctccgat ggagcagtaa ccttcgggat
     8641 cctcgccgat tacataacag acatcggcac ccggccgacc gaccacctcg ctcccgtaga
     8701 ccccggcatg acgcctatcg accggcgata cctggtggga gtcacagacg aatcggtgat
     8761 cgtcctcgac gcagcggcac tccttgcctc tcccgggatg gtgatagacg aggccaggaa
     8821 ataacgccgc gttaaccggc cgttatgccg aaaacccgcc aggaatcgct tttcccggga
     8881 tggatcgtta tttaaccccg ggtgtgcatt attctagtac gtcgtacagg aacgagtgga
     8941 acggaggcaa gccatgttca cgtttttttc ggatataaag gtcgggacga agatcctcgt
     9001 catctgcctg tttctcgcga tcatcccgac gctcatgctc ggcatagtgg cgtacaccag
     9061 ctcgagcggt gtgatcaacg agcagatcga gacgctgctt gagacgcagg tgcacgatgc
     9121 gagagggtgg acgaacgacg tctacaaact cacgcgcaat aaggtgaaca gcgacttaaa
     9181 cgttctccgt gagaacttct acgcccgggg cactcccgag gtcatcaacg gaaaactgac
     9241 gctcgtgaac gccggcggcg agccctacgt catcaacgat aacttcgaga tcgtcgacca
     9301 ggtgcagtcc ctggtcggcg gtgcggcgac cgtcttccag gtttatgaca acaacgccat
     9361 ccgcatatcc acgaacgtca tcgggaccga cgggtcgagg gcggtcggga ccgagctgac
     9421 ccagaacgtc tacgatgtcg tggtgaacca gggggagacc tactacggga gcagagacct
     9481 ctttggcaaa cgctacgtca ccgcatacga accgataaaa gacagcagcg ggaagatcgt
     9541 cggggtcctc ttcgtcggga cggaggagga agagaccctc gatgtcgtca aggcaagcct
     9601 caaggatacg gtcatcggca ggaacggcta catgttcgtc atcgacagca acggccaggt
     9661 gctcgtgcac ccctccctcg agggagagaa ctggtcgagc gaggccttcg tccaggagat
     9721 actccagaag aaggtcggca tcatccgcca ccaggtcgac gggacggata tgatcgacgc
     9781 ctacacctac tacgaaccgc tcgactggca catcgtctcg cgtgccgaac tctccgactt
     9841 caccgggccg atcgacacca tccggagcac gatcgtcatc gtcgtcctcg catccatcgc
     9901 catcggtgtt gcgatagcca tcctcttcgg ccgctcgata tccgatccgc tccagcaggt
     9961 cgtgctgatg ctcaaagaac tcaggaacgg ccatctctcc gcccggctca acatccggcg
    10021 gcaggacgag atcggtgtca tggcaaggac gatggatgag tttgccggcg acctccagac
    10081 gaacgtcgtc gggaacttaa aaaagatcgc cctcggcgag tacgtccagg agttcccggt
    10141 cgccgatgag ggcgacgaga tccgccccgc acttgcgctg atggtccagt ccctcgacca
    10201 tctccacaag gagacgctca aactcacgga tgccgcccgt gcgggcgacc tctccatccg
    10261 cggggacgag aaagcgttcc gcgggggata ccggatgatc atcgccgggt tcaacaagac
    10321 actcgaatcg ataaccgaac cggtgaacga ggcgatgcgg ctcgcgagga tctacgcatc
    10381 cggcgacttc accgcccggt tcgatgaaaa gataccggtt gccggagagt tcgtcgccta
    10441 ccgcgacgcc ctcaacacga tcggcatcga gctctcgcgt ctgatgaagc tgatcagcga
    10501 ggaactctat gaaggggtct cggtcctctc gcttgcatca aacgagatcc ttgcggtcac
    10561 gacccagctt gccgcggcca gttcgcagac cgccgcaacg gtgaacgaga cgtccgactc
    10621 ggtcgagagc gtcaggaaga agaccgaact cgtgaaccag aagacgaaag gtgtctccga
    10681 gaaggcgatg cgggcgctcg aggtctcgaa aggcggccag cgatcggtgc gcgagaccct
    10741 ggagggtatc aaccagatcc agcggcagat ggacatcatc cggatgaacg tcatcaggct
    10801 ctcggagcag agccaggccg tcggcgagat catcgccacc gtgaccgata tatccgagca
    10861 gtccaacctc ctcgcggtga acgcttccat cgaggctgca aaggccggcg agttcgggag
    10921 agggtttgcg gtcgtcgcaa acgagatcca taacctcgcc gaacagtcga agaaggcgac
    10981 atcgaacatc agggtcattc tgaccgatat tcagcagggc atatcctcga ccgcggcctc
    11041 gaccgagcag gggacccggt cggttgcgga tgcgacccgg ttgacgaaca acgcccagga
    11101 ggcgatcgag gtgctcgccc ggtcgatagc ggatacgtcg caggaggtca tcgagatcgc
    11161 cacgtcgatc caggcgcagg cttcaggagt cgaccagatc tccagtgcga tggagaacat
    11221 ccgcgatgcg gcacagaaga acctcgagac cacccacaag gcggagaaga ccgccgggga
    11281 cctgcacgag ctcggcgctc gcctgaagag gatcacccag cagtaccggg tctgacacga
    11341 cgtgccatga gcgggacgga cgaacaattc cgcgcccgcc tcctcgatat gttccgcgag
    11401 gaggcggacg agtaccttga agcgatcgcc gcagggttga tcgagctcga gaagaccggg
    11461 ctcgtgcccg aactggtcga gtcggtctac cggaggatcc acagcctgaa aggcgcctcg
    11521 cgggcggtca acctccgcga gatcgagtcc atctgccaga acctcgagac catcttctct
    11581 ctcgtgaaac ggggcgagta cacccccggc gtgaacgact tcgacctctt ccaccggacg
    11641 ctcgcggtcg tgaaggcgct tctccaggaa gagcagcccg acgcatcgcc ggcagagatc
    11701 gtcggagcgc tccgggccat ccccggcaaa cccgccggga gcggcccggg tccggccctc
    11761 cgggaacccc acgctcccga ggctcgcaac ggcaacgatc cggccgtaac agggccggat
    11821 cggggcacag tgcggatcgc ggcccacaaa ctcgaccggc ttaccgccag cgccgatggc
    11881 cttctcacgg cgcagatatc catcatgcag cggatacgcg agctcgagga gatggtcgcc
    11941 cgctttaccc tctggcagcg gaaccactcc caggcgttca acgacctgaa cctgctccag
    12001 aggaaagtct ttcgtgagga gaaggcggcg ctcccccacg acctggtcct gccgctccag
    12061 agggcggccg agtttttaga gtacaaccgg gagttcgtga cctacctgca gcacgacctt
    12121 gccacgcacc tgcgggccat ggagatcgac cggtcggcgc tcaaggcgag caccgccgag
    12181 atctcagacc tgatccacga cgccgcgctg cttccggcgg caagcatgct cgcgccgttc
    12241 tccgcgttcg tgagggaggc ctcccgcgca gcgggaaagt ccgtcgagct cacgatcgag
    12301 ggaggggaga tcgaggtgga ccggcgtatc ctcgatgcat tgagagaacc cgtcatgcac
    12361 ctgatccgca acagcatcga tcacggtatc gaggagccgc cggtcaggat agctcagaac
    12421 aaacctgcca ccggttccgt gcgggttcgg atcttctccc gctccggcag ccgggtcggc
    12481 atcgaggtga tcgacgacgg ggccggcatc gacagcagcg cgatccggaa gaccgccgtc
    12541 gagagcgggg tgatcacgaa ggaagaggat gcggccctca cggatagcga ggcgatctgg
    12601 ctcatcttcc ggtcggggat gacgacgagc cggatcgtca ccgacctctc ggggaggggc
    12661 cttggacttg caatcgtcga ggataccgtg tcccgcctcg gggggcgtat gacggtatcc
    12721 tcggtcgtcg gcaggggaac caccatcagt ttctccgtcc cggtgaggat gaccaccctc
    12781 cgcggcctgc tcgtccgttc cgggaggcag gtctacgtca tccccataca gcaggtgaaa
    12841 caggtcctcc gggtgcaccc cgactccctg acttcctcgc ggggccggcc gacgatccgg
    12901 ttctccgggg agacgatcga ggtcatcagg ctcaccgata tcctgggcat ccctccgtcc
    12961 ggcccccctc ccgcggacac gcggccaacc cccctcgtca tcatcgccta cggggcgggg
    13021 cagatcgcct gcacggtcga cgaggtgatc cgggtgcagg agatcgtcgt ccggccgctc
    13081 gggagccagc tccgctccgt ccggaggatc gagggggccg ttatcctcgg agacggcagc
    13141 gtggcgctcg ttctcgaccc tctcgaactg atccaggatg cgctgcaggc ggataggccg
    13201 gcacccgggc ccgcactccc gggagaggcg gggtggcgga tactcgtcgt cgaggattcg
    13261 gtcacatcga gggtcctgct gcagacaatc ctcgaggagg ccgggcatca ggtagagacc
    13321 gccgtcgacg gcatagatgc attcgcaagg cttaagcagc atgagtttga tatggtcgtc
    13381 tcggatgtag atatgccccg gatgaacggt tttacgctca ccgagaagat acgcgcagaa
    13441 ggccggcagt ttgccggact ccgggtggcg ctggtcacct cgctcgattc acccgaagac
    13501 cgggagcggg ggagggcggt cggggcggat gcctatgtcg tcaagagcaa cttcaatacg
    13561 gaagactttc ttgagacggt gcgccgcctg atgcgccgcc gctatggaga ggtgcgatga
    13621 catgagagat acagacaaca gaacgacaat gagcgtcctg gtggtggagg atagccggac
    13681 acaggccgag cttatccgcc acatcctcga gaaggaggga tacgacgtcg cccttgccgc
    13741 ggatggcgcc agggcgctgc aacggatagc ggataaccga cccgacatcg tcctatccga
    13801 catcatgatg ccggggatgg acggatacga gctctgccgc cggatcaagc gggattttcc
    13861 ggatatcccg gtcatactgg tcaccaacct cttcgacccc gccgacgtga tgcgggggct
    13921 tgcatccggg gccgacagct tcatagtcaa acccgtcgat ccccggcacg tccgttccca
    13981 gatcgaggcc gtccagaggg cggcagaggg gtccgacccg gacggcaccc ctgccgggct
    14041 cgatgtctcc ttctccggca acacctatac catcaccgcc cggaggcgca cgatcttaaa
    14101 caccctcctc tcgacctaca ccgtcgcggt ggcaaagaac accgaactcc aggaggcgca
    14161 ggaacaactc cactccttaa acgaccggct ccaggaggcc gtcgatgacc tctcgcgcag
    14221 caacgagagg cttgcggcgg agaaccagga acggcggcgc gtcgaaaagg cgcttgcgga
    14281 tgcaaaccga aaactccagc tcatggcgag catcacccgc cacgacctct taaaccagct
    14341 tgcggcgctc ggggggtatc tcgatctggc ccagatgatg cgcgagagag agccggcaaa
    14401 cgcatggaag caggtcgcga gcgccggcga gatcgtgaac cggatcagca acaccatccg
    14461 atttaccgcc gagtaccaga aggtcgggtc ggcgtccccc gcctggcagg atatccggtc
    14521 gctcgtggag aagtcggagc ggtatgtctc ggcggaaacc gtgaccctcg tgaacgccgt
    14581 gccggcgggc gcggaggtat acgccgatcc gctcatcgag aaggtcttct caaacctcat
    14641 cgagaacgcg gtgcgctacg ggaagacggc gaccaccgtc acattcagcc tgagaaggag
    14701 tggggatacc tgtaccatcc tctgcgagga tgacggggtg ggtgttccgt atgacgagaa
    14761 agagaagata ttcacctacg catacggcat gaacaccggg ctcggcctct tcctcgcacg
    14821 cgagatcctc gccatcaccg gcatcgccat cagggagacg ggagagccgg ggaggggcgc
    14881 ccggttcgag atcctctgtc cggtgggggt gatccgtgct cccgaacggc ataccgggtg
    14941 agtatcggcc agggactcgc gcccggaata tacaggtatc ctcatgaacg ccggagactc
    15001 tctcaccgca tacttctccg gcaggcggaa ccgaaccgcg accgtagccg gactgaccgt
    15061 tctgaccctc ggcgggagcc tgttcggcat gatcctcgga gctcccgcaa acctcatccc
    15121 tgccctcctg ctcagcatac cgatcatact cgcgagttac tggtatccgc ggcgcggcac
    15181 cctcttctcc gcctgcatcg cggccgccta caccgtcacg gccttcctct tctccccgcc
    15241 cgtcgcccac cttgctctca gcgtcctccc ccgtgcggcc ctccttatcc tggtcggcgg
    15301 cgtggtcgcg ttcctctcga cgcggctccg ggagtccgag cagcagatga accagatcat
    15361 cgagttcctg cccgacgcga cattcgccat cgatctcgaa ggaagagtca ttacctggaa
    15421 ccacgcgatc gaggatatga ccggggtgaa gaaggatgcc atgatcgccc gcggcgatca
    15481 cgagtatgcc atacccatct acggtgaacg gcgggcgctg ctcgccgacc tggtcctcca
    15541 gggcgacccc gggagcgggt atccctcggt caggagggag ggggatatct tcattgcgga
    15601 ggcccgcgcc ccgcacctcc ggggcggccg gggagcgcag atcaggtttg cggcaaccac
    15661 gcttctcgac cacgagggga gggtcaccgg agccatcgag tccatccggg acatcaccga
    15721 cgaggtcatg atggaggccg cgctgcagaa caccaaccgc cagctcaaca ccctctcggg
    15781 tatcctgcgc accgacctct cgaacaggct tgcggtcctg tacgggcatc tctcggtggg
    15841 cgcgatgaag ttcgacgacc tcgaggtcct ctcgttcatc gatgacctga aggatgcggc
    15901 aaacggcatc cagcggcaga tagagatctc ccgcgagttc cgtaacatcg ggacgtcgcc
    15961 gccggcgtgg atccccgtac agcagatcgc caggaacgcg gcggataccc tctcctttgc
    16021 cggcgtctcc cttgagatat gggtggagag gctcgaagtc tttgccgacc cccatctcgg
    16081 gacggtcttt acccacctct ttgagaactc gcttgctccg gcgacgggcg cgacacgggt
    16141 cgtcgtcacc taccatctgc gcccggacgg ttgtgcaatc atcgtcgagg acgacggaac
    16201 cgggattccc gacgcggcaa aagaggggct ctttgtccag aaggagaagg ggtacgggcg
    16261 cggtctcttc ctcgctcacg agatcctggc catcaccggg atcaggatcc gcgagaccgg
    16321 aaggtttggg gcgggggcga ggttcgaact cctcgtgccg cccgaaggat acaggataat
    16381 ctgaggtgtt cgggatggca acggatattc gttcaggggc ccggccggat gtgcgcgaac
    16441 tcaggagcgc tgagttttcc gccgcagatg aggtatggcg cgagtaccgc ggtatggtcg
    16501 gcgatcccgg ccgggacagg atatttgccg tcttcctcgc gggaaagatc gtctctctcg
    16561 cccggtgcag gaggcacgcc gacggcctgg aggtcgacgg cgtcttcacg cccgagcagt
    16621 atcgcggcag agggtatgcc cgcatggcgg tcgcggctct ggtcgtggcc tgccacaacg
    16681 acgacctcta catgtacgcg gtcgagaacc ttaccgggct ttacgcggag ttcaggttta
    16741 tgtcgattcc tgagcgcgac cttccgcccg gcgtccgcga acgctacacc tgggcggcgg
    16801 ggaacgtgga gggggctgaa gtccggccga tgtaccggag agccgggctt taggaacggg
    16861 tagcccgcct ccggggcgaa ccgggtatcc atccccaccg ggcaccccgg aagtaacata
    16921 tggagcggtg caggagaggt gaccatgcac aggaccatct ctcttgcgtt tgccatcctc
    16981 atcgccgcca ccactcttgc cggatgcacc ggggtgccgg gccccgagga gacggaggtg
    17041 ttcgaccgga cggttgccgg ggagccggaa agcagcgtcg tcgtggtcaa ccggaacggc
    17101 ggcgtgaacg tgagcgcctg ggatgaggat tacgtcgcca ttacggcggt caaacgaacc
    17161 gtctacggcc ggggcgaact tgcgaaggtc agtatagacg tgacggaggg cgaccccctc
    17221 cggatagaga gcgtgtacac cggcataaac gtccgggcag gcgtggacta cacgatacga
    17281 ctgcccccag acgtcgtcct gcaacgggtc gagagctcga acgggggtat cgaacttacg
    17341 ggcgtgcggg tggccggaac ggaacttgcg acgtcgaacg gcccggtgcg cgtggccggt
    17401 gcccgcggcg gcgatattcg cgtgtcgacg agcaacggcg ggatcgatct ccgcgacgtc
    17461 gagggctacg tcgtgggaac gacgagcaac ggcgggataa cggtcgaaga ttgcggcggc
    17521 cttatcgcgt tgcggacgtc aaacggcccc atatcggccg atatctccgc agtccgggag
    17581 gacgtgacga tatcgacgag caacggcggt ataaccctcc ggttcgcaga ggatctcaat
    17641 gcccgcgtga tcgccaccac ctcgaacggc agggtgacga tccgcgatcg ttcgctccag
    17701 gtcagcgagt cgaccgggac ctcggtcacg ggtctgctcg gcgacggggg gccgacgatc
    17761 accgccacca catcgaacgg cggtatcgac ctctcgggcc tctgaccggc aagcacaacc
    17821 cttatcccct gccgatccga gagtacccat acgggagcag acgacgatgg cagacgtcta
    17881 tttcgcgaat ctccgggcga ggagccacca cgagagcaag atacagaaga tccgccgcct
    17941 ctttgatgcg gccgggtttg acagggtggt tcgcccgggc gaccttacgg ccgtcaaact
    18001 ccacgtcggg gaacgggggt gcgacaccta cataaacccg atctttgccc ggcaggtggt
    18061 cgataaggtg aaggagcgcc gggcccaccc gttcatcacc gataccggca ccctctatgc
    18121 ggggagccgg gccgacgccg tccggcacac cgtcaccgcg atcgagcacg ggtttgatta
    18181 cgcagtcgtc ggcgctccgg tgatcatcgc cgacggcata tcgggcggct actggcgtga
    18241 ggtcgcggtc ggcgggaacc atttcgagaa cgtcaggatc gccggcggca tcctcgatgc
    18301 cgagagcatg ctcgtcctct cgcacgtcaa aggccacgac ctcgccgggt ttgggggcgc
    18361 tatcaagaac ctggcgatgg gctgcgcccc gccctcgggg aaggcggagc agcacgccgg
    18421 ccggccgtgc ttcgagactg aggcctgcaa ggggtgcggg aggtgcgtcc aggtctgccc
    18481 gcaggcggcg gtgaccctgg tcgaggggcg ggcgcggttc aacccggagc gctgcgtcgg
    18541 gtgcggcgac tgcatgcgct cctgcggaga gggggcgatc gagtttgact ggacgacgga
    18601 gatccggccg ttcatcgaga ggctctgcga gtacgccctc ggcgccgtcc gcgacaaacc
    18661 cggaagggtg ggctatatca acttcctcct tgcgatcacc cccgactgcg actgcgtccc
    18721 ctggagcgat gcggcaatcg tccccgatat cgggatcctc gcctccaccg atccggtcgc
    18781 gatcgaccac gcgagcttcg acctcgtcaa cagcgaacgg gggattgccc ggacggctct
    18841 cggggagcat accgccccgg gggaagacaa gttcagggga atctgggact acacggacgg
    18901 cggttaccag atcgactacg cggctcggat cgggcttggc gacgcatcct accggctgat
    18961 cgagatctga gagggaggtg tgatctgtgg gtctcgcgcc cggtgagagg acccgggcat
    19021 cccctaaccg gtcgggaacg cccgcatcac ccgtgccctg acggcctctt tcgggagcgc
    19081 gccgaccacc cggtcgatga gcatgccgtt tgcaaagaac aggagcgtcg gtatggccgt
    19141 tatgctgaac tgtgtcgata tcgcgcggtt ctggtcgacg ttgcacttgc cgaaggtcac
    19201 ccggccgtca aagtcacgcg caagatcctc gatcaccggg gccaccatcc ggcaggggcc
    19261 gcaccactcc gcccagagat
//
LOCUS       DAOSEE010000025        18849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1210_length_18849_cov_243.478557, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000025 DAOSEE010000000
VERSION     DAOSEE010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 18849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18849
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1210_length_18849_cov_243.478557"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PLI76_03320"
     CDS             complement(<1..314)
                     /locus_tag="PLI76_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61091.1"
                     /translation="MEYARVLSGREDPLPIFSKVVEALDNFEEFPFLLEPIYREASDL
                     GDEDLDRFRFGLVRLQVYADIHRYEDMEAAQRMKYVAATIERVLFGRLLLEGEEAGGK
                     QC"
     gene            complement(318..1220)
                     /locus_tag="PLI76_03325"
     CDS             complement(318..1220)
                     /locus_tag="PLI76_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOI61092.1"
                     /translation="MTQTNEPNKDTCSGDCAGCPSATKCDDQRNVGAPKGLPPKADVM
                     VKHVVLVLSGKGGVGKSTVAANLAYALANQGYNTGLIDLDIHGPDIPKMLGIEDARLQ
                     SYDGKVIEPVRVTGNLAIISMAFLLPERNTPVIWRGPMKMTVIKQFLEDVNWGSLDYL
                     VVDLPPGTGDEALTVAQLAPNIAGAVIVTTPQDVAILDSSKAVQFIKKLELPVLGIVE
                     NMSGFICPHCKEEIDIFGKGGGEKEAKELGVPFLGAIPLDPDMRKAADEGRPFIIRQA
                     GSAENPTWKSIDAVMKALVKQIEE"
     gene            complement(1599..2036)
                     /gene="tsaA"
                     /locus_tag="PLI76_03330"
     CDS             complement(1599..2036)
                     /gene="tsaA"
                     /locus_tag="PLI76_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HOI61093.1"
                     /translation="MAPIECTPIGVVRSPYRARGDAPRQGRLADTVAEIHVFDAYAPG
                     LEDVERSSHLIVLYWLDRAERGLLYAKPPGETRARGVFSTRSPARPNPIGFGIVDLVR
                     RDGDILVVRGLDALDGTPVLDIKPYSPEIDCIPEATGGWHIKK"
     gene            complement(2039..2482)
                     /locus_tag="PLI76_03335"
     CDS             complement(2039..2482)
                     /locus_tag="PLI76_03335"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HOI61094.1"
                     /translation="MTQKETGGGTGEVTLYTDGASRGNPGDAAWAYVIVRDGAVAASS
                     SGFIGKATNNVAEYHAVINGLRAAREFTRGHLEVRSDSELVVRQLTGRYRITKEHLAA
                     LADEVRQWSRHFTKVRFENVPREDPCIQVADRLCNEVLDAEGRRR"
     gene            2588..3292
                     /locus_tag="PLI76_03340"
     CDS             2588..3292
                     /locus_tag="PLI76_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOI61095.1"
                     /translation="MLDTGRIIEDKTMRGRLSVFADRTDAGRRLAATLSQLETVAEPV
                     VCAIPAGGVPPGLEVARALQAPLRMAVVRKVQIPWNPEAGFGAVTWDGRVFLNRALMA
                     GLGLSEAEVGAAVAKARKSVEERVEMFSGGRPEPGLFGRTAILVDDGLATGYTMLAAV
                     EAVRSGSPREVIVAVPTGSLHAVEFIARKADLVVCPNIRTGRTFAVAQAYERWHDITD
                     REVQELLIQARDMGLF"
     gene            3470..3958
                     /locus_tag="PLI76_03345"
     CDS             3470..3958
                     /locus_tag="PLI76_03345"
                     /inference="COORDINATES: protein motif:HMM:NF013340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HOI61096.1"
                     /translation="MSMQDLKVEIDFDHFPPEYTCDGEGISPRIRITGSGAPYLAIII
                     DDPDASKGTFTHWVAWNIPSTEEIPPGIPPEPRISHPVSIVQGMNDSRKTGYTGPCPS
                     KGVHRYFIRVWGVERELDLPPGSERVELTDALASAATGYGEAMATYGREKVLKSPPAT
                     RT"
     gene            4172..4627
                     /locus_tag="PLI76_03350"
     CDS             4172..4627
                     /locus_tag="PLI76_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HOI61097.1"
                     /translation="MPKLTVKLDFEDFPDVYTCKGANRSPPIRVEGILPNIKSLAVLA
                     TTSPEEGPSKVAWILWNLPAVPLIPEGFPAGGVVESPLHAVQGTNDFGTVGYRGPCPE
                     TGKGEAYLFRVYALDLDLALAPGANWEEMIRKMQGSLNQSGEAIAFAVR"
     gene            complement(4667..5119)
                     /locus_tag="PLI76_03355"
     CDS             complement(4667..5119)
                     /locus_tag="PLI76_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOI61098.1"
                     /translation="MGLVKCCREQVVAVSPDTPAVEVARIMGEKNVGSVVIVTGDNRP
                     TGVITDRDLTIRVMAHEKNPGEVRAGDITTRDVITFRDSMGIYEAIQKMTAEGIRRMP
                     VVDDAGELIGIVTMDDIVRMLGEEMAAIAKNIEKQSPPLQREAPPIPM"
     gene            complement(5197..5490)
                     /locus_tag="PLI76_03360"
     CDS             complement(5197..5490)
                     /locus_tag="PLI76_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61099.1"
                     /translation="MHRPVEKVASFAATTARSLEIVANSAPMGSGDGVVARTISRGRS
                     PPTKKTATIGPPVARTSALSMLEAISKTERPATVRRMGSRSIIPDVQPLADRI"
     gene            5568..6143
                     /locus_tag="PLI76_03365"
     CDS             5568..6143
                     /locus_tag="PLI76_03365"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOI61100.1"
                     /translation="MTKRSIGPRTLLYPHPVLIIGTYDVSGRPNAMAAAWGGICSSRP
                     PAVAISVQKARQTYENLVEQREFTISIPPVDYVREADYFGIVSGHDTDKFAATGLTPV
                     KADRVNAPYVGEFPVVLECRLLQTVEIGVHTQFIGEILDVKVDESVIGPDGKPDIGKI
                     RSFAYDSMRQEYYGFAGVVAKAFSAGKEVKR"
     gene            complement(6172..6333)
                     /locus_tag="PLI76_03370"
     CDS             complement(6172..6333)
                     /locus_tag="PLI76_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61101.1"
                     /translation="MTANSLLCRIGLHRWGKKRGYDEYSSNVIEWEQRCERCGKRKRW
                     VEAKRDRRR"
     gene            complement(6330..7448)
                     /locus_tag="PLI76_03375"
     CDS             complement(6330..7448)
                     /locus_tag="PLI76_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04084 family radical SAM/SPASM
                     domain-containing protein"
                     /protein_id="HOI61102.1"
                     /translation="MYYHLILTDNCNLCCTYCRGKAFTVDPPELPDITVDEDLPVDLD
                     IDLSDLYRFLAKDPDAVLTFYGGEPLLAADRIREIARDAPVSALILHTNGTLLDRLEP
                     RVANRFDTILVSIDGPEALTDAHRGEGTFGLITKNLRNLVAGGFAGELIARMTVTEDT
                     DIVEAVSYLAENDRFSFPAIHWQMDANFWSDYSLRDYAAWVTESYNPGIRSLVALWVE
                     TMQEEGRVLRWYPFMDPMQDMLLSRPSMLRCGCGHANYSIMTDGQIAPCPIMVGMRDY
                     YVGHIATADPLRLPVTTVGSPCTECRLLDFCGGRCLYSNITRPWPEAGRRVVCETVEN
                     LYQALSAALPEVRALIGAGRVGMEDFNHRKYNSCEIIP"
     gene            7581..7913
                     /locus_tag="PLI76_03380"
     CDS             7581..7913
                     /locus_tag="PLI76_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOI61103.1"
                     /translation="MSKAKENLAEQVIDPRALVAYQSGSIVSRMLVYTKAGTITVFAF
                     DAGEGLSEHTAPYDAVLQVLDGEALITIADKEYPMKAGELIIMPAKIPHAVHAIAPFK
                     MMLTMIHA"
     gene            8099..10855
                     /locus_tag="PLI76_03385"
     CDS             8099..10855
                     /locus_tag="PLI76_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOI61104.1"
                     /translation="MVPLLSPAESRATAWHALSAAEVAGRLDSPEGGLSGAEVTRRRE
                     IFGENALPTKRSPAVIEIFLRQFASPLIYVLLAAGVVSVFFADAADAVFIFAVILLNA
                     VIGTSQEVKAERGATALQQMLRVRARVRRDGREATVPAENLVPGDRAILESGDRVPAD
                     IRVTRAIALSIDESLLTGESHAVGKNPDAVDPSLPPADRLNMLYAGSTVMSGRAMGIV
                     VATARNTEIGQIAETVTASEAGKPPLTIRMEDFSRKISIAVLAATGLLAFIVLGQGMP
                     PFEVFFLAIALAVSAIPEGLPVALTVALSIATSRMAGRNVVVRYLAAVESLGSCTLIA
                     SDKTGTLTVNQQTARVVALPTGEVFEVTGAGYSGEGDVVDESGTPVAGVPRAHLEHLA
                     RAAAINNEATLERTGEGDWAHRGDAIDVAFLALTYKLGLDPAGIREDAPPVAGLPFES
                     ERRYAAVWYRKGEEILVAVKGAAETVVPFCRTMAGGSGGNVPLDPDRVQKGHDNLTSR
                     GFRVLAVAHGRVEGTFSAGEPELPPLELLGLVGFIDPIRPDVPDAVRRCRSAGVDVVM
                     VTGDHPATALAIARELRIADSPDEVVTGAELGDEETSTFLQFVDRVKHARVFARVTPL
                     QKLRIVEAYRESGAFVAVTGDGVNDAPALRSANIGIAMGSGTDVARDTASLIITDDNF
                     ASIVAGIEEGRYAYDNVRKVVYLLISTGFAEVLLFMLALAMGLPLPLIAVQLLWLNLV
                     TNGIQDVALAFEGGEPGVMDRPPRRPEEGIFNRLMMEEVLLSGTVIALVTLGVWYWLL
                     SNGWDEFAARNLLVLLLVLFENFHVFNCRSEHLSAFRIPISNNYLLIAGVIAAQGIHI
                     LALYTPVLRDVLQVQPVSLVEWLSLLVLASSVVVVMEVFKAVRKRRHSGHQTGYGGGE
                     APSR"
     gene            complement(10813..12702)
                     /locus_tag="PLI76_03390"
     CDS             complement(10813..12702)
                     /locus_tag="PLI76_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HOI61105.1"
                     /translation="MENWRSVAAAALGCLVVAAIIGTALASDEAGTDTLGDLQKFGSK
                     EEIEVFLKENTQKGWNGYPGAPGGAGRETGMNAPAPMATAAPTSSTRDYSTTNVQVEG
                     VDEADFVKNDGKYIYILSGGTLAIVEAFPAKDAKILSETRIEGTPEALFLAGDRLVVF
                     AVATEEEMITPAGSVTPVPISRTVTHAHVYDIRDRSGPELIRTLTFTGDYYDARMVGE
                     YVYALTRESPLWVRDDLALPAVREGEAPPIRPEIYRPKTPMQNYVFYTESAFSVRGTG
                     TPDAETFLLGYDTTLFASQENLYIGYRNMGPAYPAGGTPERTIIHRFAIGQGKIGYRA
                     MGEVPGHLLNQFSLDEHEKNLRVATTVDGWTREGSIQYNNVYVLNSAMETIGTLEHIA
                     PGERIYAARFVGDRLYLVTFKQIDPLFVIDLSDPKHPGILGELKIPGYSDYLHPYDAD
                     HIIGIGKETDENGWGGVSVAGLKIALFDVSNVNKPIQVDAVVIGEAGTDSAALHDHKA
                     FLFAKEKGLLVVPVSEIVRVENPASKYPGSYGTATWQGAYVYRVSPASGFTLEGKVTH
                     AEKGPYPVWDASDAVRRSLYMDDTLYTVSARSIVMTGLGDGKRMNEVFLPYREGASPP
                     PYPVW"
     gene            complement(12796..13731)
                     /locus_tag="PLI76_03395"
     CDS             complement(12796..13731)
                     /locus_tag="PLI76_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HOI61106.1"
                     /translation="MIITVIILVVGIAILVKGADLFVSGGSGLALHHRISPALIGSTI
                     IAFGTSLPELVVSTDAAATGNTGIALGNVLGSNIANIALVLALCTLIRPGMVAASGTS
                     RSALVRHTALMLGATAAFTLLAMRGTLDAQTGVVLLILFAIILYLLLRERREESVPDI
                     RSHGWADALYIGLGLVAVIVGARLVVDSAVSLADAFGIPAFVIGLSIVAVGTSLPELA
                     TSLVAAIKNEGAISIGNILGSNIFNLLLVLGISLLLAPVAVGSFADIIAVALFSVAIL
                     PLLFARPSVVRAWSIVLLIGYAAYIAWIFSTAPVG"
     gene            13996..14697
                     /locus_tag="PLI76_03400"
     CDS             13996..14697
                     /locus_tag="PLI76_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOI61107.1"
                     /translation="MLSFDEEIDALEQERELLLRFPEEEFIAIIRDVGFSCDCCGRCC
                     TREFNGHVFLLEDDTDRVLSFQPDALMPAPDFDACDQKGRFYVSGYALRTKPDGSCIF
                     LEDGRCSIYDRRFSICRVYPYMLHREADERGVVDWRQIGGLNEHGCYNTPIDDAECAR
                     IARETRAYEAAFLDQEIRFKRALRDLFARDGLRYVRRTYDLLMRDFRKGKEVEVRVFH
                     RGRFYPHRVACDSYE"
     gene            14928..16298
                     /locus_tag="PLI76_03405"
     CDS             14928..16298
                     /locus_tag="PLI76_03405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOI61108.1"
                     /translation="MEREYDVVVIGTGVAGSDVAWHCGDAGMRVAITDRREYGGTCAL
                     RGCVPKKVLAGAAEVVARVHDQQGNGVRGTVSIDWSALIAFVRTFTGPTPRRKEESLR
                     EAGVDTYHESARFVGPNRVMIGDDTLTARQIVIAAGAHPRPLGVPGADLVTSSDDFFY
                     LETLPDRIVFIGGGYISFELAHVAARAGSEVTILQRSGRVLKGFDPDIVDRLVLASRE
                     IGIDVQVNMPLVSVERTTAGLLVRAGKKGEESTFEADMVVHGAGRVSTTEGLGLAAGN
                     VATDRRGIVVDDHLRSVSNPAVSVAGDANPTSPQLTPVAVMDAHIVVDNILNGNTRIA
                     DYSVVPSAVFTAPPLASVGLTEEAAQKKGISYVVTAGDLSARFTIRSIGQKHAGYKLL
                     VEKDSRRILGAHLIGPHVEEVINIFALAIKHNLTVDDLTLDAIPWAYPSSTYEVIHMI
                     HPLVRK"
     gene            complement(16459..17124)
                     /locus_tag="PLI76_03410"
     CDS             complement(16459..17124)
                     /locus_tag="PLI76_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HOI61109.1"
                     /translation="MHPEVIIHNSVSLDHAVRGFDIDLDLHYGALLALEPEAVLVGSA
                     TARSGLDMFHDIGKPEAESDRHRPAVRPDDPRPLCAIVDSRGVLKNLLHFYRNMEHTR
                     DVVVLVSEATPGDYIDYLREREYPFIRCGADRVDLRGAVRELEERFGVARVVSDSGPD
                     LNDALIREGIVDTISLIVYPVIAGEGEKKLFGRAGGRVALELQTVVPMESGAVHLMYT
                     AKR"
     gene            17268..18473
                     /locus_tag="PLI76_03415"
     CDS             17268..18473
                     /locus_tag="PLI76_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOI61110.1"
                     /translation="MEIPHSIADRMAGRAADRESILSPRATRNADYLRRSGHRLEEPV
                     IRPPFSRDADRIVHSKAYSRYIDKTQVFYLVENDHITHRVLHVQLASRIGRTIGRALG
                     LNDDLIEAIALGHDIGHVPFGHLGEEILDGLCRENGIGRFRHNVQSVRFLDTVEDCDL
                     TVQVLDGILCHNGEVHDRSLRAEGEADWDSFEEKLQRIEDGGDALPCTAEGCVVRVAD
                     SISYLGRDLADALEVAVIDGRDLEGFPENCMDLFHIAGRKREDFSEINRRVLDVLIKD
                     IVMTSYDADCVAFSAEASASVAAFKEFNMSHIYENPRLIANREKIRFMFRYLFDHILE
                     DVEAERENSPIYEELIHAPWVSPRYIATATPAELARDFIAGMTDRYFERVFRQSILPE
                     RVRSRYRGG"
     gene            18592..18738
                     /locus_tag="PLI76_03420"
     CDS             18592..18738
                     /locus_tag="PLI76_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61111.1"
                     /translation="MKFTPSKEKLTEILREFEPNRELADAIEKASKEMRQAQVREVDF
                     DADT"
BASE COUNT         3285 a         6012 c         6040 g         3512 t
ORIGIN      
        1 cagcactgct tcccgccggc ctcttctccc tcgaggagga gccggccgaa gagcactcgc
       61 tcgatcgttg cggccacgta cttcatccgc tgggccgcct ccatatcctc gtagcggtgg
      121 atgtccgcgt agacctgcag gcggacgagg ccgaaccgga agcggtcgag atcctcgtcc
      181 ccaaggtcgg aagcctcacg gtagatcggt tcgagcagga aggggaactc ctcgaagtta
      241 tcgagcgcct cgacaacctt tgagaatatt gggagggggt cctctcgccc ggaaagaacc
      301 ctcgcgtact ccattggtca ctcctcgatc tgcttgacca gggctttcat gacggcatcg
      361 atgctcttcc aggtcgggtt ctcggcgctc ccggcctggc ggatgatgaa gggccgccct
      421 tcgtccgcgg ccttccgcat atcgggatcg agcgggatgg cgccgaggaa gggaaccccg
      481 agctcctttg cttctttttc gccgccgcct ttgccgaaga tatcgatctc ctccttgcag
      541 tgggggcaga taaagccgct catgttctcc acgataccga ggaccgggag ctcgagtttc
      601 ttgatgaact ggacggcctt gctcgagtcg agtatcgcga cgtcctgcgg ggtggtgacg
      661 atgacggccc ccgcgatgtt cggggcgagc tgggcgacgg tgagcgcctc gtcgccggtt
      721 ccgggcggga ggtcgacgac caggtagtcg aggctgcccc agttgacgtc ctccaggaac
      781 tgcttgatga ccgtcatctt catcgggccg cgccagatca cgggggtgtt gcgctccggg
      841 agcaaaaacg ccatggagat gatcgcgagg ttgccggtga ccctgaccgg ttcgatgacc
      901 tttccgtcgt aggactggag gcgggcatcc tcgatgccga gcatcttcgg tatatccggg
      961 ccgtggatgt caaggtcgat gaggcccgtg ttgtagccct ggttggcaag ggcgtaggca
     1021 aggttcgccg ctaccgtgct cttgccgacc ccccctttcc cggagaggac gaggacgaca
     1081 tgctttacca tgacatcggc cttcgggggg aggccctttg gagcgcccac gtttctctgg
     1141 tcatcgcact tggtcgcaga cggacagccg gcgcagtctc cggaacaggt atctttattg
     1201 ggttcattcg tttgagtcat cagaattatc ctccacgatg tgatatcctt cgctaaggaa
     1261 gatctagagc tactatatat tagcggcaag cgtcacaaaa aggtataggt gatggcaaaa
     1321 tttcctgtat gcctcgggga ataccgcttt tcggcgagtg tatccgcccg ccgcaagggg
     1381 ggggaggagg gcggcaacgt tgccgtcgcc aggggtctct tacgacattc gcctggctgc
     1441 ctctgccatc ctctcaagac cccggcggtg cggagaggac gtcgccgggc ccatcccccg
     1501 gggcacctcg ccgatgccgg ccgtacagta ccggagctcc cggaatggga ccgcgatagg
     1561 gacccccgcg accccggcat catcccctct tccccggcct atttcttgat gtgccatccg
     1621 cccgtcgcct cgggtatgca gtcgatctcg ggcgagtagg gcttgatgtc cagcaccggg
     1681 gttccgtcca gggcgtcaag ccccctgacg accaggatat cgccgtcgcg ccggaccagg
     1741 tcgacgatcc cgaatccgat cgggtttggg cgtgcgggcg agcgggtgga aaagacccct
     1801 ctcgcccggg tctcccccgg cggctttgca tagaggagcc ccctctccgc ccggtcgagc
     1861 cagtagagca cgatgaggtg gctgctccgc tctacatcct caagccccgg ggcgtaagca
     1921 tcgaagacgt ggatctccgc gacggtatcc gcgaggcgcc cctggcgcgg ggcgtcaccc
     1981 ctggcccggt agggcgaccg gacgacgccg atgggggtgc actcgatcgg ggccatatct
     2041 accgcctcct cccctccgca tcgagcacct cgttgcagag gcggtcggcg acctggatgc
     2101 aggggtcctc cctcgggacg ttctcgaacc tgacctttgt gaagtggcga gaccactgcc
     2161 gcacctcatc cgcaagggcc gccaggtgct ccttcgtgat gcggtaccgg cccgtgagct
     2221 ggcggacgac cagctccgag tccgaccgga cctcaaggtg cccccgggtg aactcccgtg
     2281 cggcgcgaag gccgttgatg acggcgtggt actcggcaac gttgttggtc gccttcccga
     2341 tgaaccccga actactggcg gcgacggcgc cgtcccgcac gatcacgtat gcccaggcgg
     2401 catccccggg gttcccccgc gatgcaccgt cggtgtacag cgtcacctca ccggtgcccc
     2461 ctcccgtctc tttctgtgtc atctgccgat cgatcatcga tctctggagg gttattgata
     2521 tcgctttccg gggtgaaggg aagtccaccc cgaaatgtta ataccccggc gggttaaggg
     2581 cggacccatg ctggatacgg gccggatcat tgaggataaa acgatgcggg gtcgattgag
     2641 cgtttttgcg gatcggaccg atgccgggcg ccgcctcgcc gcaacgctct cgcagctcga
     2701 gacggttgcc gaaccggtgg tctgtgccat ccccgccggc ggggtgccgc cggggcttga
     2761 ggtggcacgg gcgctccagg cacccctccg gatggcagtg gtgcggaagg tgcagatccc
     2821 ctggaacccc gaggccgggt tcggtgccgt gacctgggac gggcgggtct tcctgaaccg
     2881 cgccctgatg gccggactcg gtctctcgga ggctgaagtg ggcgctgccg tcgcaaaggc
     2941 caggaagagc gtggaggagc gcgtggagat gttctccggc gggcggcccg aacccgggct
     3001 tttcggtcgg accgcgatcc tggtcgacga cggcctcgcg acagggtaca cgatgcttgc
     3061 cgccgtcgag gcggtccggt ccgggtcacc ccgcgaagtc atcgtggcgg tccccacagg
     3121 ctccctccat gccgtcgagt tcatcgcccg gaaggccgac ctcgtcgtct gcccgaacat
     3181 aaggaccggc cgcaccttcg cggtggcaca ggcctacgag cggtggcacg acatcaccga
     3241 ccgggaggtg caggagttgt tgatccaggc ccgggatatg gggctctttt gataacccgg
     3301 agccggaacg gtccgggaaa cggccccggc accacccgct gccggcgccc ctgcttgcgg
     3361 gtgagtcgcg tcgccgccgc ttcgggttca ccgggctttg ggatccggcc ccgatgtccc
     3421 gtaccgcaac aggtatatac gacctcccct caggggtgcc ggagaggtga tgagcatgca
     3481 ggacctgaag gtcgagatcg attttgatca ctttcccccc gagtatacct gtgatgggga
     3541 gggcatctcc ccgcggataa ggattaccgg atcaggtgcg ccctatcttg cgatcatcat
     3601 agatgatccg gatgcttcga agggcacatt cacgcactgg gttgcctgga atataccgtc
     3661 gacggaggag atcccaccgg gcatcccccc ggagccccgg atatcccatc cggtctccat
     3721 tgtccagggg atgaacgact cccggaagac aggttacacc ggcccctgcc cctcgaaggg
     3781 tgtgcaccgg tacttcatac gggtctgggg cgtcgagaga gagctcgatc tcccgcccgg
     3841 atccgagcgg gtagagctta cggatgcgct cgcctccgcc gccaccggct acggtgaagc
     3901 gatggcgact tacggccgtg agaaggtgct caagtccccg ccggcgaccc gaacctgaga
     3961 tctcctccac cccccctcca atctttcggg actccgcccg gttcggtctc cacctccccc
     4021 gggcgagagg atcgatggat ctcacgctcg ttcccgcggc gggcgccggc agatacattc
     4081 ttatcgatac catgcagggg ttcctcctct ccatggccgg acaaaccttt ttcacgtccc
     4141 cgacagatct ctaccaacgg agacgtgctg tatgccgaaa ctgaccgtga aactggactt
     4201 tgaagatttc cccgacgttt acacctgcaa gggggcgaac cgttcccctc ccatccgggt
     4261 ggaagggatc ctcccgaaca tcaagtcgct tgcggtcctc gcgacgacga gccccgagga
     4321 ggggccgtcg aaggtggcgt ggatcctctg gaacctcccg gcggtccccc tgatcccgga
     4381 gggattcccg gccggcgggg tggtggagtc gccgctccat gccgtccagg gcacaaacga
     4441 cttcggcacc gtcggctacc ggggcccctg cccggagacc gggaagggcg aggcctacct
     4501 cttccgggtc tacgcgctcg acctcgacct tgcgctcgcg cccggggcga actgggaaga
     4561 gatgatacga aagatgcagg ggtcgctgaa ccagtccggg gaggctatcg cgtttgccgt
     4621 aaggtagggg cggggggttc ccgccagaac aggggtgccg gccccatcac atgggtatgg
     4681 gcggggcctc tctctgcagg ggcggactct gcttctcgat attctttgcg atagcggcca
     4741 tctcctcccc gagcatgcgg acgatgtcgt ccatggtgac gatcccgatg agttccccgg
     4801 catcgtcgac gaccggcatt cgccggatgc cttcagcggt catcttctgg atggcctcgt
     4861 atatccccat gctgtcccgg aacgtgatca cgtcccgggt cgtgatatcc cccgcccgga
     4921 cctcgcccgg gttcttctcg tgcgccatca cccggatcgt gaggtcgcgg tcggtgatca
     4981 cccctgtggg ccggttgtcg ccggtgacga tgacgacact tcccacgttc ttctctccca
     5041 tgatcctcgc cacctctacc gccggcgtat ccggcgagac ggcgacgacc tgctcacggc
     5101 agcatttcac aagtcccata tcgttacctc ctctggcgcc tcatccatgc cggtatgata
     5161 tatgaacgta acccgtacac ccccggcctt ctccccctat atgcgatcgg cgagaggctg
     5221 tacatcgggg atgatggacc ggctgcccat ccttctgacc gtagcgggtc tctccgtctt
     5281 tgagatcgcc tcgagcatcg ataacgccga cgtcctcgcg acggggggcc cgatcgttgc
     5341 ggtctttttg gtcggagggc ttctcccgcg gctgatcgtc cgggcgacga cgccgtcgcc
     5401 tgaccccatc ggcgcactgt tcgcgacgat ctcaagcgac ctcgcggtcg tggccgcgaa
     5461 agaggctact ttctctaccg gtcggtgcac ctccggggga ggggggcggg cgctgcatga
     5521 cccgggtcaa aaaggtttat cccggtccag gggcgaaccg ttatcctatg acgaagcgat
     5581 cgatcggtcc acggaccctc ctctaccctc acccggtctt gatcatcggg acctacgacg
     5641 tctccggccg gccgaatgcc atggccgccg catggggcgg tatctgctct tcccgccccc
     5701 cggcggtcgc gatatcggtc cagaaggccc ggcagaccta cgagaacctg gtcgagcagc
     5761 gcgagttcac gatcagcata cccccggtgg actacgtcag ggaggccgac tacttcggca
     5821 tcgtctccgg ccacgatacg gataaattcg cggcgaccgg gcttacgccg gtgaaggccg
     5881 accgcgtgaa cgctccctac gtaggggagt tcccggtcgt cctcgagtgc cggctcctcc
     5941 agacggtcga gatcggggtg catacccagt tcatcggcga gatcctggac gtgaaggtgg
     6001 acgagagcgt catcgggccg gacggcaaac ccgatatcgg aaagatccgg tcgttcgcct
     6061 acgactcgat gcggcaggag tactacggct ttgccggcgt cgtcgcgaag gccttctcgg
     6121 cggggaagga ggtcaaaagg taactctttt tgtatccgtg cggtatcccc gtcaccgccg
     6181 gcggtcccgt ttcgcctcga cccaccgctt ccgcttcccg caccgttcgc accgctgctc
     6241 ccactcgatg acgttcgagg agtactcgtc gtatccgcgc tttttacccc agcggtgaag
     6301 cccgatcctg cagaggaggc tgtttgccgt catggtatga tctcgcagct gttgtacttc
     6361 cggtgattaa agtcctccat cccgacccgc cccgcgccga tgagggccct gacctccggg
     6421 agtgccgccg agagcgcttg atagaggttc tccaccgtct cgcagacgac ccgccgcccg
     6481 gcttccggcc aggggcgggt gatattcgag tagaggcacc ggccgccgca gaagtcgagg
     6541 agccggcact ccgtgcaggg gctccccacg gtcgtcaccg ggaggcgaag agggtcggcc
     6601 gtggcgatat gcccgacgta gtagtccctc ataccgacca tgatgggaca gggtgcgatc
     6661 tgtccgtcgg tcatgatgct gtagttcgcg tggccgcacc cgcaccggag catgctcggc
     6721 cgggagagga gcatatcctg catcgggtcc atgaagggat accaccggag cacccggccc
     6781 tcctcctgca tcgtctcgac ccagagcgcg acgagcgatc tgatgccggg gttgtaactc
     6841 tccgtcaccc atgcggcgta atcgcgcagg gagtaatcgc tccagaagtt tgcgtccatc
     6901 tgccagtgga tggcaggaaa cgagaaccgg tcgttctccg caaggtagct gacggcctcg
     6961 acgatatcgg tatcctcggt cacggtcatc cgggcgatca gctcgccggc aaatcccccc
     7021 gcaacaagat tccggagatt cttcgtgatc agcccgaacg tcccctctcc gcggtgagcg
     7081 tcggtgaggg cctcggggcc gtcgatcgag accaggatgg tatcgaaccg gttcgcgacc
     7141 cggggctcga ggcggtcgag gagggtgccg ttcgtgtgca ggatcagcgc cgataccggg
     7201 gcgtcgcggg cgatctcgcg tatgcgatcg gcggcaagca ggggctctcc gccgtagaag
     7261 gtcaggacgg cgtcggggtc ctttgcgagg aaccggtaga ggtcggaaag atcgatatcg
     7321 aggtccaccg gaagatcttc gtcgacggtg atatcgggca gttccggggg gtcgacggtg
     7381 aacgccttcc cgcggcagta ggtgcagcag aggttgcagt tatcggtcag gatgaggtgg
     7441 tagtacactg aaggtctctt acaggttggg ggcagggcgg gaaaaagata aggatacggg
     7501 gcatcccggc gccacaccgg cggcgagccc ccaccacacc cggcgcaaaa cagaagttcc
     7561 cacaggataa atggactcgt atgtccaaag caaaagagaa ccttgccgaa caggtcatcg
     7621 acccccgtgc cctcgtcgcc taccagtcgg gctcgatcgt cagccggatg ctcgtctaca
     7681 cgaaggccgg caccatcacg gtctttgcgt tcgacgcggg cgaagggctc tcggagcaca
     7741 ccgcacctta cgacgccgtt ctccaggtcc tcgacgggga ggcgctcatc acgatcgcgg
     7801 acaaggagta tcccatgaag gcgggtgaac tgatcatcat gccggcgaag attccccacg
     7861 cggtccacgc catcgcgccg ttcaagatga tgctgacgat gattcacgcc tgacccgccg
     7921 ggaggagggg ttgccggtca ccgcctgcaa ctatttatgc gccggcgatg gatccccttc
     7981 cgtgcatatt ctctggaagt ggtatcgccg gcctcgccct ggagaaccgc gtagcctgct
     8041 cgaaccgatc ccgaaggctg cacctgtacc ctgcggcgta tcctcgtgag gcggcggcat
     8101 ggttcctctc ctctccccgg ccgaatcccg ggcgacggca tggcatgccc tttcggccgc
     8161 cgaggtagcc gggcggctcg attcgccgga gggaggcctc tccggcgccg aggtcacccg
     8221 acggcgagag atcttcggtg agaacgccct ccccacgaag cgttctcctg cggtcatcga
     8281 gatatttctg cgccagttcg caagcccgct catttatgtc ctcctcgcgg caggagtcgt
     8341 atccgtcttc tttgccgatg ccgccgatgc ggtcttcatc ttcgcggtca tcctgctcaa
     8401 cgccgtcatc gggacctccc aggaagtgaa ggctgagcgg ggcgcaacgg cgctgcagca
     8461 gatgctccgg gtccgggctc gggtgaggcg agacggtcgt gaggcgactg ttccggccga
     8521 gaacctggtt cccggcgacc gcgccatcct ggaatccggc gaccgtgttc ccgccgacat
     8581 ccgtgttacg agagcaattg cgctctccat cgatgagtcg ctcctgaccg gcgagtctca
     8641 cgcggtcggg aagaaccccg atgcggtcga cccctcgctg cccccggccg accgcctgaa
     8701 catgctctat gccggcagca ccgtcatgag cggtcgggcc atgggaatcg tggttgccac
     8761 agcccggaat accgagatcg ggcagatcgc tgagacggtc acggcgtcgg aggcgggcaa
     8821 acccccgctc accatccgga tggaggattt ctcccgaaag atcagcatcg ccgtccttgc
     8881 cgctacggga ctgctcgcgt tcattgtcct cggtcagggg atgcccccgt ttgaggtctt
     8941 cttcctcgcc atcgcgcttg cggtctcggc gatcccggag ggcctcccgg tcgctctgac
     9001 tgtcgcgctc tcgattgcga cgtcacgtat ggccgggcga aacgtcgtcg tccggtacct
     9061 cgccgccgtg gagagcctcg ggagctgcac cctgatagcg agcgataaga ccgggaccct
     9121 cacggtgaac cagcagaccg cccgggtcgt tgccctgccc accggcgagg tcttcgaggt
     9181 caccggggcg gggtactcgg gcgaaggaga tgtggttgat gagagcggca ccccggtggc
     9241 cggtgtcccc cgcgcccatc tggaacacct ggcccgggcc gcagcgatca acaacgaggc
     9301 aaccctggaa cgaaccggcg aaggagactg ggcgcaccgg ggcgatgcga tcgatgtggc
     9361 gttcctcgcg ctcacctaca aactgggtct ggatcctgcc ggcatccgcg aagatgcgcc
     9421 tcctgttgcc ggactcccct tcgagtccga gcggcggtat gccgccgtct ggtaccggaa
     9481 gggagaagag atcctggtgg cggtcaaggg ggccgccgag acggtcgttc cgttctgccg
     9541 caccatggcc ggcggatcgg gcggcaatgt accgctcgac ccggatcgcg tgcaaaaagg
     9601 gcacgacaac ctcacctccc ggggctttcg ggttcttgcc gtggctcatg gccgcgtcga
     9661 aggcacattc tctgcaggag agccggaact gccgccgctc gaactccttg gcctcgtcgg
     9721 attcatcgac ccgatccggc ccgacgttcc cgatgccgta cggcgatgcc gcagcgccgg
     9781 agtcgacgtg gtgatggtga ccggagacca tccggcaacg gctctcgcca tagcacggga
     9841 actccggatc gcggattctc ccgacgaggt cgtcaccggc gcggaactgg gtgacgaaga
     9901 gacctcgact ttcctccagt tcgtcgaccg ggtgaagcac gcccgggtct ttgcccgggt
     9961 gacgccgctg cagaaactcc ggatcgtcga ggcctaccgt gagagcgggg cgttcgtcgc
    10021 ggtcaccggg gatggggtca acgatgcgcc ggcgctccgg agcgcgaaca tcgggatcgc
    10081 gatgggctcc gggaccgacg ttgcccggga tacggcatcg ctgatcatca ccgacgacaa
    10141 cttcgcctcg atcgtcgccg ggatagaaga gggccgctat gcctacgata acgtccggaa
    10201 agtcgtctac ctcctgatat cgaccgggtt tgcagaagtc ctcctcttta tgctcgccct
    10261 tgccatgggc ctgccgctcc ccctcatagc cgtccagctc ctctggctga acctggtgac
    10321 gaacggcatt caggacgtgg cgctggcatt tgaagggggc gagcccggcg tcatggatcg
    10381 gccgccgcgg aggccggagg aggggatctt caatcgattg atgatggagg aggtgctgct
    10441 ctccggcacg gtcattgcgc tcgtcacgct cggtgtctgg tactggcttc tctcgaacgg
    10501 gtgggacgag ttcgcggccc ggaacctgct cgtcctgctt ttggtgctct ttgagaactt
    10561 ccacgtcttc aactgccgtt ccgaacacct ctctgcgttc cgcatcccca tcagcaacaa
    10621 ctacctcctg atcgccggtg tcatagccgc gcagggtatc catatcctcg ctctctacac
    10681 gcccgtcctc cgggatgtgc tccaggtgca gcccgtttca ctggtcgagt ggctctcgct
    10741 cctcgtcctt gcctcctcgg tcgtggtcgt tatggaggtc ttcaaggcgg tcaggaaacg
    10801 ccgtcattcc ggtcaccaga ccgggtacgg gggcggggag gccccctccc ggtaggggag
    10861 gaagacctcg ttcatccgtt taccgtcgcc aagcccggtc atgacgatgc ttcgcgccga
    10921 gacggtgtag agggtgtcgt ccatgtagag cgaccggcgc accgcgtcgg acgcatccca
    10981 gacggggtac ggccctttct cggcgtgggt gacctttcct tcgagggtga acccggaggc
    11041 ggggctcacc cggtagacgt aggccccctg ccatgttgcg gtgccgtaag agccggggta
    11101 cttcgatgcg gggttttcca cccgcactat ctcgctcacc gggacgacga ggagaccttt
    11161 ctcctttgca aagaggaagg ccttgtgatc gtggagcgcc gcggagtcgg tcccggcctc
    11221 gccgataacg acggcgtcga cctggatggg cttgttcacg ttcgagacgt cgaagagggc
    11281 gatcttgaga cccgcgaccg agacgccgcc ccagccgttc tcgtccgtct ccttcccgat
    11341 accgatgatg tggtcggcgt catagggatg gaggtagtcg gagtacccgg ggatcttgag
    11401 ttctccgagg atgcccgggt gcttcgggtc cgagagatcg atgacgaaga gagggtcgat
    11461 ctgcttgaac gtcacgaggt acagccggtc gccgacgaac cgggcagcgt atatccgttc
    11521 ccccggcgcg atgtgttcga gcgtcccgat ggtctccatc gcgctgttga ggacgtagac
    11581 gttgttgtac tggatcgagc cctcccgcgt ccagccatcg acggtcgtcg ctacccggag
    11641 gttcttttcg tgctcgtcga gcgagaactg gttcagaagg tgcccgggaa cctcgcccat
    11701 cgcacggtag ccgatcttcc cctgcccgat cgcgaaccgg tggatgatcg tccgctcggg
    11761 ggtgccgccg gcgggatatg ccggccccat gttccggtag ccgatgtaga ggttctcctg
    11821 cgacgcaaag agagtggtgt cgtagccgag gaggaacgtc tcggcatcgg gggtgcccgt
    11881 gcccctgacg gagaaggcgc tttctgtgta gaagacgtag ttctgcatgg gagtcttcgg
    11941 gcggtagatc tcgggtcgga tgggcggcgc ttcaccctcc ctcaccgcgg ggagggcgag
    12001 gtcgtcccga acccagaggg gcgactctcg cgtgagggcg tagacgtact ccccgaccat
    12061 cctggcgtcg tagtagtcgc cggtgaaggt cagggtccgg atcagctccg ggccggaccg
    12121 atcacggata tcgtagacgt gtgcgtgcgt caccgtccgc gagatgggga ccggcgtcac
    12181 gcttcctgcc ggtgtgatca tctcttcttc agttgcgacg gcaaaaacca ccagacggtc
    12241 gcccgcgagg aagagtgcct ccggggtgcc ttcgatccgg gtctcggata ggatcttcgc
    12301 atcttttgcc gggaacgcct cgacgatcgc gagggttccg cccgagagga tgtagatgta
    12361 cttcccatcg ttcttcacga agtcggcctc atccacgccc tccacctgca cgttcgtcgt
    12421 ggagtagtcc cgggtagaag acgtcggggc ggcggtcgcc atcggcgccg gggcgttcat
    12481 cccggtctcc cggccggcac cccccggggc cccggggtag ccgttccacc ctttctgcgt
    12541 gttctccttc aagaacactt cgatctcctc tttcgacccg aacttttgca ggtcgcccag
    12601 ggtatcggtc ccggcctcat ccgacgcaag ggcggtcccg atgatcgctg cgaccacgag
    12661 acagccgagg gcggctgccg caaccgatct ccagttttcc atactcgttc accttccgat
    12721 gaaggaagat gggcgcgccg gagcatcagg tttgtgtaac ctacgaatat tttcgaagcc
    12781 tcgttgagaa cactctcacc cgaccggcgc cgtcgagaag atccaggcga tgtaggccgc
    12841 gtagccgatg agcaggacaa ttgaccaggc tctgacgacg gacggccggg caaagagcag
    12901 ggggaggatc gcgaccgaga agagggcgac ggcgatgata tcggcaaacg acccgacggc
    12961 gaccggggcg aggaggaggc ttatcccgag aaccagaaga aggttgaata tattgctccc
    13021 gaggatgttg ccgatcgata tggcgccttc attcttgata gcagccacga gggatgtcgc
    13081 gagctccggg agcgacgtgc cgacggcgac gatcgagagg ccgatgacga atgccgggat
    13141 cccgaaggca tccgcgagtg agacagcact gtcgacgacc agtcgggcgc cgacgatgac
    13201 ggcgacgagg ccgaggccga tgtagagcgc gtcggcccac ccgtgagacc tgatatcggg
    13261 aacgctttct tcacggcgct cccgcaggag aaggtagagg atgatcgcaa agaggatgag
    13321 gagtacaacc cctgtctggg cgtcgagcgt acctctcata gcgaggaggg tgaaagccgc
    13381 cgtcgcaccg agcatcaggg cggtgtgccg gacgagcgct gagcgtgacg tccccgacgc
    13441 ggcaaccatg ccgggtcgga tgagggtgca gagcgcgagg acgagggcga tgtttgctat
    13501 gttgctcccg aggacatttc cgagggcaat ccccgtgttc cccgtggctg ccgcatccgt
    13561 gctgacgacg agctccggga gcgatgtgcc gaacgcgatg atcgtggatc ctatcagggc
    13621 cggggatatg cggtggtgga gagcaagacc gctcccaccg ctcacgaaga gatcggctcc
    13681 tttgacgagg atggcgatgc cgacgacgag tataatgacg gttattatca tgggagcgcc
    13741 ccggatgtag tggtagtact tgggcggagt tctcctaata cttttcccga tccgggccct
    13801 ctacagcctc tcccgggccg ggtgccggcg aagagacgat ggtgcctccc cgctgccgcc
    13861 cgtggcgccg cacaggatgc ctgcccctcc ggtgcacccc ggggtgccgg aggagatatc
    13921 cgccatgccg tcgaatactt gagggtatca gtcccggcac acctgccggg ctcacgatgg
    13981 ggaggttgct gccgtatgct ctcgtttgac gaagagatag atgccctcga gcaggaacgc
    14041 gagctgcttc tccggttccc tgaggaggag ttcatcgcta tcatccgcga cgtggggttc
    14101 tcctgcgact gctgcggccg gtgctgcacc cgggagttca acggccacgt ctttctgctc
    14161 gaggatgaca ccgaccgggt cctatcgttt cagcccgatg ccctcatgcc ggcgccggac
    14221 ttcgatgcct gcgaccagaa gggacggttc tacgtctccg gctacgcact ccggacaaag
    14281 ccggacggtt cctgcatctt ccttgaggat ggcaggtgca gcatctacga ccggcggttc
    14341 tcgatctgcc gggtctatcc ctacatgctc caccgggagg ccgatgagcg gggcgtcgtc
    14401 gactggcggc agatcggcgg cctgaacgag cacgggtgct acaacacccc gatcgacgat
    14461 gccgaatgcg cccggatagc ccgggagacc cgggcatacg aggcggcgtt cctcgaccag
    14521 gagatccggt tcaaaagagc cctccgtgac ctctttgcgc gtgatgggtt gcgctacgtc
    14581 cggcggacct acgacctcct gatgcgggat ttccggaagg gaaaggaagt cgaggtcagg
    14641 gtctttcacc gcgggcggtt ctacccgcac cgggtcgcct gcgatagtta cgaatgatgg
    14701 aatccccggc ggagaccggg gttccccccg gtgccgccag gcccggcatg gtcatcccta
    14761 tccgggtctt ctccgattct ccgtgcggca actgtcgtcg caatgatcgt gcccttcgct
    14821 ctctctccag ggagagcggg agctgtttta cctgcggcct gacgggctct cctctcccgt
    14881 gatccgggta aacctttata ccgctctatg gcgactgggg ccccactatg gagcgggagt
    14941 acgatgtcgt cgtcatcggg accggggtcg ccggttctga tgtcgcctgg cactgcggag
    15001 atgccggtat gcgggtggcg atcaccgacc ggcgggagta tggggggacg tgcgctctcc
    15061 ggggttgtgt cccaaagaag gtgctcgccg gggctgccga ggtggttgca cgcgttcatg
    15121 atcagcaggg gaacggggtc cgcggaaccg tctcgatcga ctggtcggct ctcatcgcct
    15181 ttgtgcgcac gttcaccggt cccacccccc gacggaagga ggagagtctc cgggaggcag
    15241 gggtcgatac ttaccacgaa tctgcccgct ttgtcggccc gaaccgggtg atgatcggcg
    15301 acgatacgct cacggcccgt cagatcgtca tcgctgccgg cgcacatccc cggccgctcg
    15361 gtgtcccggg tgcggatctc gtgacgtcaa gcgacgactt cttctacctc gagacgcttc
    15421 ccgaccgtat cgtcttcatc ggcggaggat acatctcctt tgaactcgcc catgtggccg
    15481 ccagggccgg gtcggaagtg accatcctcc agcggagcgg acgggtcttg aaagggttcg
    15541 atcccgatat cgtcgaccgg ttggtgctgg cctcaaggga gatcgggatt gacgtccagg
    15601 tgaacatgcc cctcgtctca gtcgagagaa ccaccgccgg tcttctggtg cgggccggaa
    15661 agaaaggcga agagagtact ttcgaggccg atatggtcgt ccacggcgcc ggccgggtct
    15721 ctacgaccga aggtctcggc cttgcggcag gaaatgtcgc gaccgaccgc cgggggatcg
    15781 tcgtcgacga ccacctccgg agcgtctcaa atcccgccgt ctccgttgcc ggagatgcga
    15841 accccaccag cccacagttg acgccggtgg cggtgatgga tgcccatatc gtcgtcgaca
    15901 acatattgaa cggcaacacc cggatcgcgg actactcggt cgtcccgagc gccgtcttca
    15961 cggccccgcc cctcgcctcg gtcgggctca cggaagaggc ggcgcagaag aaggggatat
    16021 cgtacgtcgt caccgccggc gacctctcgg cgcgtttcac aatccggagc atcggccaga
    16081 agcacgccgg ctacaaactc ctggtcgaga aggactcccg ccggatactc ggggctcacc
    16141 tgatcgggcc gcacgtcgag gaggtgatca acatattcgc cctcgcgatc aagcacaacc
    16201 tgacggtgga cgacctcacg ctcgatgcca tcccgtgggc ctacccgagc agcacctacg
    16261 aggtcatcca catgatccat cccctggtgc gaaagtgatg agttccccgg cccggggccg
    16321 gccacatgcg ggtccatgtg ccaaagaccc ctgcggctcc ggatgctccc caccgcgctc
    16381 tccttctcca gggatcccct gcgtgggtca gtacaccccc gccgcggtca tgcggttccg
    16441 gactacagat cccatcactt atctcttcgc cgtatacatc aggtgaaccg cgccggactc
    16501 catcggcacg accgtctgga gttccagcgc cacccgcccg ccggcccggc cgaagagttt
    16561 cttctccccc tcgccggcaa tgaccgggta gacgatcagg ctgatcgtat ccacaatccc
    16621 ctcccggatc agggcatcgt ttaagtcggg gccggaatcg gagaccaccc ttgcgacacc
    16681 gaacctctcc tcgagttccc gtacggctcc ccggaggtct acccgatcgg cgccgcaccg
    16741 gatgaaaggg tactcccgct cgcggaggta gtcgatgtaa tcccccggcg tcgcctccga
    16801 gacgaggacg acgacatctc tggtatgctc catgttccgg tagaagtgga gcaggttctt
    16861 gagcacgccc ctgctgtcga cgatggcaca gaggggacgg ggatcgtccg gccgcaccgc
    16921 gggccggtgc cggtcagact ccgcctcggg tttgccgata tcgtggaaca tgtcgagccc
    16981 ggaccttgcc gttgccgagc ccacgaggac cgcctccggc tcgagggcga gcagtgcgcc
    17041 gtagtgcagg tcaagatcga tatcaaagcc ccgtaccgcg tgatccaggc tcacgctgtt
    17101 gtggatgatg acttccgggt gcatggcgga aggctatacg tgcccgggat agagccggtg
    17161 cggattcgcc tcctgaaccg atacagaaag ccctatctct tctcccggcc gaataaccct
    17221 ttaccggaag ttctcccggc gaggagcacc gtaccggagg agaccagatg gagatcccgc
    17281 acagcatcgc agatcggatg gccgggagag cggccgaccg tgaatcaatc ctctcgcccc
    17341 gggcgacccg gaacgccgat tatctcagga ggtccggcca caggctcgag gaaccggtga
    17401 tccggccgcc gttctcgcgc gacgccgacc ggatcgtcca ctcgaaggcc tactcgcgct
    17461 acatcgacaa gacgcaggtc ttctacctgg tagagaacga ccacatcacc caccgcgtcc
    17521 tccacgtcca gcttgcatca aggataggga ggacgatcgg gagggctctc ggcctgaacg
    17581 acgacctcat cgaggcgatc gccctcggcc acgacatcgg ccacgtgccc ttcggccacc
    17641 tcggggagga gatcctcgac ggcctctgcc gggagaacgg gatcgggagg ttccggcaca
    17701 acgtccagag cgtccggttc ctcgataccg tcgaggactg cgacctcacg gtgcaggtgc
    17761 tggacgggat cctctgtcac aacggagagg ttcatgaccg gagcctcagg gccgagggtg
    17821 aggccgactg ggactcgttt gaggagaaac tgcagaggat cgaggatggg ggcgacgccc
    17881 ttccctgcac cgctgaaggg tgtgtggtgc gggtcgcaga cagcatatcg tacctcggcc
    17941 gcgatcttgc ggatgccctt gaggtcgcgg tcatcgatgg gcgggatctt gaagggttcc
    18001 cggagaactg tatggacctc tttcacatcg ccgggagaaa gagggaggac ttctcggaga
    18061 tcaaccggag ggtgcttgac gtcctcatca aagacatcgt catgacgagt tacgatgccg
    18121 actgcgtcgc cttctccgct gaggcatcgg cctccgtcgc ggccttcaaa gagttcaaca
    18181 tgagccacat ctacgaaaat ccgcggctca tcgcgaaccg tgagaagatc cggtttatgt
    18241 tccgttacct cttcgaccac attcttgagg atgtcgaggc ggagcgcgag aactcgccga
    18301 tctatgaaga gctgatccac gccccctggg tatccccgcg gtatatcgcc accgcgacgc
    18361 cggcggaact tgcgcgggac ttcatcgccg gcatgacgga tcgctacttc gaacgggtct
    18421 tccggcagtc catcctcccg gagcgagtca gatcgaggta ccggggtgga tgaaccctta
    18481 tacccatgca gaggtatggg gcacgaagtc gatcagcata tccgacgagg cgtacgagag
    18541 gctcacctgg ctgaagggat catcgagggt gagtttttcc gaggccattc cgtgaagttt
    18601 acaccctcaa aggagaaact cacggagatc ctccgtgagt ttgaaccgaa ccgcgaactg
    18661 gcggatgcga tcgagaaggc gtcaaaggag atgcggcagg cgcaggtgcg cgaggtcgac
    18721 ttcgatgccg atacttgata cttcctggtc gatccgattc ggaaaaaacc tgaggcacta
    18781 acaaagatcg aggcattgga ggcaacgggc tctcccctta agacaacaac ggtcgacatc
    18841 ctggagctc
//
LOCUS       DAOSEE010000026        18823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1212_length_18823_cov_235.143009, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000026 DAOSEE010000000
VERSION     DAOSEE010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 18823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18823
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1212_length_18823_cov_235.143009"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PLI76_03425"
     CDS             complement(<1..391)
                     /locus_tag="PLI76_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOI61112.1"
                     /translation="MTRPCTIVISGKGGTGKTTIAALLIDALIAAGERPILAVDADPN
                     ANLHEALGIALTETLGGMREEAFTRAIPPGMDRTGYVKYRFRRALAEAEGYDLLAMGR
                     PEGSGCYCFPNALLTECIGTLERGYRFV"
     gene            complement(388..1413)
                     /locus_tag="PLI76_03430"
     CDS             complement(388..1413)
                     /locus_tag="PLI76_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61113.1"
                     /translation="MIPPPELLATGVGALPHRDPIEACRAVLAAFPRIPYVPTLPNRG
                     FLEAIVFADAEHLPGGVVRDGRLVIDRSTDPSEAMEQVLLDYLERNAEPYRVGKAYGS
                     GFHAMMGRDLSDALLVKCQVTGPVTFGMQVVDEARRPILYDPEYADLLGKLLGLRARW
                     CETAMRERMGARATLVVLSEPYLASLGSSVVPVDAGVAASAFEDIAALLDGGLGIHCC
                     ANTDWGFVLGLAPAVVSVDAWTYAREFLLYADEIARYMERGGVVAWGIVPADHAAFAA
                     EKPDAFFSRFEEIRARATETIDPDLFDRQSLITPTCGIRNANEEEAIEIMAATASLSR
                     RLRGEEP"
     gene            complement(1410..3344)
                     /locus_tag="PLI76_03435"
     CDS             complement(1410..3344)
                     /locus_tag="PLI76_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOI61114.1"
                     /translation="MEDVMPTVTFLPGYRKVTVPEGTTLLDAARAAGLSMNVVCGGQG
                     KCGKCLVFIKEGTVTFDREKYRRFFSDDEIARGACLACQATVVGDIQVNVPAESLIQE
                     QKILVEVPGLEGIPFNPAVRKYEVRLEPPSLDDTTPDLTRVLEGIELQGGPPPTSIYA
                     PLDVLCYLPLVLRRSGWHATATVALAPVGYRLSSVDEGNTTGRLYGAAVDLGTTTVVV
                     YLWSLVDGRILATASNYNRQISCGEDILSRVNFSRKSGLSRLQQLAAESINEALTTAA
                     DAAGIDREEIFEVMIAGNTVMTHLFLGIDPAYMIAEPYIPVVRRMLSTGAGQSGIAAH
                     KNAEVYTFPAVSNFIGGDIIADILATGMAEREEPTLMVDIGTNFEAVLGNSDWMLSCA
                     GAAGPALEGGEVLFGMRANPGAIERARIDAETLAPTCSTINGARPRGICGSGLIDLLA
                     ELFRACVIDRTGRINLEIGHPRVRKGQYYPEFVVVWKKETSLGKDIVITENDIRNLIM
                     SKAAIHGACMTLLDAAGLMPGDIGRVCFAGAFGNYLNKENAITIGLIPEISLERVENI
                     GNGAITGANIALLDRKRRKELDAIARKITYIELNAEPSFMDRYTSSCFLPHTDFTLFP
                     HVQQTLEACRARQGVRSWQA"
     gene            complement(3332..4666)
                     /gene="acsC"
                     /locus_tag="PLI76_03440"
     CDS             complement(3332..4666)
                     /gene="acsC"
                     /locus_tag="PLI76_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HOI61115.1"
                     /translation="MAMKALDIYKHLPKTNCKKCGYPTCLAFAMKLAVGKADVEACPD
                     LEAEARTLLGGVTRPPVRLVTIGTGERSVAVGEEFVLFRHEKTFYHPPGIVVQVSDAW
                     PDDEVRSVVEMVEETVVHRVGQDLVLNGVAVKYESNLPERFAEVVTLAGGGNSLPLAL
                     IADDPGALEAALAVAGHYRPLIHAATEENHEALAALAKRYGCPLAVRAADPAELAALA
                     GKCADAGVPDIVLDPAPSSLAGFVAIATDIRKAAIGRTEPALGYPLSIDAGAFGDADA
                     AIAAGILKYAGIIVVPPLSPASVEAALTLRQNIYTDPQKPIQVTPGIYPINEPGRNAP
                     VLLTVNFSLTYFTLLGYLEASRIPCYLFIADTEGLSVLTAVAGGKLTETLVADSIKEF
                     GVGDLVDHRLLVIPGYAAPLSGKIEDATGWKVLVGPRDAADLPGYLETEWKT"
     gene            complement(4670..5578)
                     /locus_tag="PLI76_03445"
     CDS             complement(4670..5578)
                     /locus_tag="PLI76_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HOI61116.1"
                     /translation="MESNDERLAIREVRLGATRAEGGTREVSYAIGGERDLPFSGKRP
                     ERLLVALEICDDPQFWPPVVRDYVGDLANDTAEWARAAERTYGADLVRLNFTSTKQRG
                     FDAFGAIAATTEEVLAATARPLIVEGSNEPELDSEVFRRSGEAGEGERLLLGTAEADR
                     YRSVAAAALAYGHAVIAQSPIDVNLAKQLNILLRETGVPQDRIVIDPYTGALGYGFEY
                     TYSVMERIRLAALAGDADLAMPMISAPTDTLSIREVREAAPADQEAMAVAWEFYTAYA
                     AVVAGASIVCVRHPLTVARLKTALEG"
     gene            complement(5594..7630)
                     /gene="acsB"
                     /locus_tag="PLI76_03450"
     CDS             complement(5594..7630)
                     /gene="acsB"
                     /locus_tag="PLI76_03450"
                     /EC_number="2.3.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit alpha/beta"
                     /protein_id="HOI61117.1"
                     /translation="MKDRLTDSLVTAIEHGRTGLDARMQALAGKLAYPETAYALPVAY
                     AVTGIAVRDANGAREAYRQSGNNLLVACECLMAGEGGVASPYTGFIADAVLRRLGYTL
                     VDGSVTGLGLLVGTPESPDAAAAICREMQEKYLLTFLAGGVVEALSGAGVRLGLDYRL
                     VPLGPESAHGIHFADILARVAMMFGGVQPGDVHSLLGYAAERAKAFVIAFPGLTDEEA
                     AFVDALRVLGIPILAAGKGYEGGEWIPVAPATAVRTGMDLRGIRVTVTAIPIPMACSP
                     AFEGKSIRKEEMYVEFGGGRTPAFELLRMRPREEVKDGKVTVVGPEVDAVPAGAALPL
                     AILVDVAGARMRADYEPVLERRIHTFVNYGEGSWHVAQRDLIWVRLSKEAVAKGVRIR
                     DIGTLLAHKLKAEFPDHIDAVQVTLVTDREQVERMLVEAREIYARRDERISGMKDEDV
                     ETFYSCTLCQTFAPNHVCVITPERPALCGAITWLDARVAHDIAPAGANQPVPKGEMVD
                     ALYGEFVGVNRFVKKASHGEVDRVSLYSMIENPMTACGCFECIAAIVPEVNGILVVSR
                     DFAGETPLGMTFSTLAGTIGGGAQTPGFIGISKNYILSDRFLAAENGIMRVVWMPSSL
                     KEELGDRLRERLAAQGVPDLFAKIADEVSAPTIEDLVLFLERVKHPALTMRSLI"
     gene            complement(7967..9028)
                     /gene="corA"
                     /locus_tag="PLI76_03455"
     CDS             complement(7967..9028)
                     /gene="corA"
                     /locus_tag="PLI76_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOI61118.1"
                     /translation="MSRHDERAEQNRMRGGQGMLISTGDTPAHSVTITVIDYDGSRVD
                     ERTCRPEDLRALIARPTVTWIDVDGVHDAGIIEAVGDVAGIHPLTLEDIANTRQRPKI
                     EDYGNYLYVAVRMLSPDGAGAFQSKQMSLVLGTGYVISFQERPGDVFSRIREHLRTGA
                     GRIRNEGADYLFYSLLDAIVDGYFTVIEEFGERIEAVEEEVVADPDHETLQAIYALKR
                     SLIALRRSVWPLRDVVAELERGDSSLIRTSTLVYLRDVYDHAIQVAETVETYRDTASG
                     TLDVYLSSQSSRMNEIMKVLTIIATIFIPLTFIAGVYGMNFAYMPELGYSWGYPVTLA
                     LMAVVAGIMLFYFKRKGWF"
     gene            complement(9073..9324)
                     /locus_tag="PLI76_03460"
     CDS             complement(9073..9324)
                     /locus_tag="PLI76_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5654 family protein"
                     /protein_id="HOI61119.1"
                     /translation="MSFKTEVIEKIAALVTAAFGLVAALAWNGAIQELFKLIFGEQST
                     LVAMFVYAVVVTIIAVIAVVLIGRAAAKAKGEDEAAERK"
     gene            9568..11580
                     /locus_tag="PLI76_03465"
     CDS             9568..11580
                     /locus_tag="PLI76_03465"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOI61120.1"
                     /translation="MDTLDDWFPYREYRPHQREMLEAAASVARDGGIAMIDAPTGSGK
                     SSVISALLAENRGRKVLVAVRTISQLATFMRELELIRKKRGGLKFAYLIGKSSMCPLG
                     GEGDVYRRCEGVKAFSTALMRERAQKGSLVPANDRHIKQQIRKMDPDHPLICPYFIRG
                     KSFVEPGDSGLKMIPSAALRTRAERVSTELIWPDRLAGFCGDICPYDTMMHAARDADV
                     ILVNFYHLFDDTIREQLYLSLGIEGSDALLLIDEAHNCGDVVQSIESVTIEERDIVQA
                     GHELAGRRRSQQSADAVTQILPQITRFMEALKASHEVEDWFDPAIFQRMILAGTLYRN
                     VEGVVDDLLKISEGIREKNMQAGEFRESAIERLTEFFYRIFRSADDPAYLTVYRKADD
                     GSVALEVRNIDPSTKLQEIAQAHACSVLISGTLSPIESYRRYYFGDLPVTTISLPNAF
                     PPENRRIFCADDITTAYSMRRDKENLARTEDYIATFAALPGNLAVYFPSYDLLNTFAE
                     RCAPRIRKKQIYVEPKDTAAASVMLREFMALPGTGRFGVLFAVSGGKWSEGLDYRGEM
                     LAGALVIGLPLAPFNRVRRMVIDYFRMKFGAEGEFISYTLPAINRALQALGRVLRTPE
                     DRGVLVLGERRFLESRVHAGLPPWMQEEMTTCTIDLFKKEAGRWRF"
     gene            11568..12011
                     /locus_tag="PLI76_03470"
     CDS             11568..12011
                     /locus_tag="PLI76_03470"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOI61121.1"
                     /translation="MAVLTGSCRFGLWHVHVAWQDDLVYRVRFARTGLEGPVPEEILL
                     YCAGRRADLSSLRSVATGGSSTFAAIYRAVRDIPSGETATYGDIAQRVGTAPRAVGSA
                     MAKNPTPIVVPCHRVVAKTGIGGFTPDPAIKEALLAMERREKGRS"
     gene            12040..12951
                     /locus_tag="PLI76_03475"
     CDS             12040..12951
                     /locus_tag="PLI76_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HOI61122.1"
                     /translation="MKIVVLGAGAVGLTIAAKLSRVADVHAVARARHADAVRERGLFL
                     TGIWGEGTYRFSCSEDLPPAWRAADYFIITAKSTDTEAVCRQFADAIRGQEVVSLQNG
                     IGNEEVIGRFTDRVIGAMIITGFEWRGDAAVHVSVEAAPMRLGRFPSGTDEAVVRLAS
                     LFSEAGIRAEATGAIRSDLWSKTLYNCALNPLGAIMNVPYGALADPHAWAVVTGIVRE
                     AYLVVEAEGIALPRATPEAYLEYLRTVQLPATAAHHSSMLQDLARGQKTEIEFMNGAV
                     AALARRHGLDAPYNACIAELIRFRERL"
     gene            12964..13599
                     /locus_tag="PLI76_03480"
     CDS             12964..13599
                     /locus_tag="PLI76_03480"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HOI61123.1"
                     /translation="MRSAIILVGGAARRAGGREKYFFTFQGKTFIDRLIDTLREVVDE
                     IVVVARDPEQCERFIHLDDVRCIPDIRRDLGPIGGLHAGTLAAHGEYIFVAACDMPCI
                     NPGVVRMLFDAALGYDAAIPSWNADMLEPLHAVYWRSALLAYLEEHESLSLRPMIRSL
                     NTRYVSVETIREIDPDLLTFTNINKVEDLQLIDPSTEHGQDDACRSGLEPS"
     gene            13936..15069
                     /locus_tag="PLI76_03485"
     CDS             13936..15069
                     /locus_tag="PLI76_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOI61124.1"
                     /translation="MLLTIFCCLAALLCGTATAGKGDYAPLWEKGADDVVSTVAISHD
                     GSLIAAGAGSTVSLLDREGDILWETAVGSRISDVAISPEGSRIGVAADKLYLFDGDGN
                     LLWAEKTTYIYRGVALSSEGISIAAACDNGALFVFDQDKNILWDYDMGTDGYGVAISE
                     NGRRIALGCDNHGVYYLNSGEGESWSYGTGNCVKGIALTPDGRFVAAGSLDRCVYLST
                     AEGEHLWKYPTTEAVLATALTEEAREVLAASGRTIYVLDQSGAERQKITLGGRVESIA
                     VTPDGSFLAAGGGDGDRSVHLFTKDAALLERMNPVEPAVNETDLPEENVTVPATLADE
                     SADTAAEVNARAGGEEVSLFSTISGWLENILSLLFKPQADFIA"
     gene            complement(15059..15904)
                     /locus_tag="PLI76_03490"
     CDS             complement(15059..15904)
                     /locus_tag="PLI76_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOI61125.1"
                     /translation="MLDVLGLEFFRNALIAGVLASIACGIIGTYVVVRRMVSVSGGIS
                     HAAFGGIGLGYFLGIDPLLGAAGFTVAAALGVGTLQLRARQQMDTLIGAVWAAGMAIG
                     ILFVYLTPGFAPDLFSYLFGNILLVPRGDILLMGILTAVIVAVVAVLYRELQAVTFDP
                     DYAAVMNLPVERLSLLLLVLIALTVVMLIRVVGIILVIALLTLPAAISRLYTARVWTM
                     MLLAVVLGIIFTVAGIALSYVIDVPSGATIVLVSTLAYAGALGAERLREGRGSESAAA
                     ASVRR"
     gene            complement(15907..16674)
                     /locus_tag="PLI76_03495"
     CDS             complement(15907..16674)
                     /locus_tag="PLI76_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI61126.1"
                     /translation="MTAPVIEVDDVWVRLRGRTILEAVSLAIHSGEFYAIIGPNGGGK
                     TTLLRVILGLLAPCRGEVRIGGSTDPAMRKDLGYVPQFRSFDFDYPITVREMVLSGRL
                     GRIARRPRRYGAEDRARTEEALDTMGIADLADRQIRNLSGGEQQRAIIARALVGDPKA
                     LLLDEPTVYVDAPTETQFYEILDGLRDRMAIILVTHDIGVLPGHVTRVACLNRRLYTH
                     DTDEITPDMIEAAYRCPVDLIAHGVPHRVLPEHSREE"
     gene            complement(16671..17525)
                     /locus_tag="PLI76_03500"
     CDS             complement(16671..17525)
                     /locus_tag="PLI76_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOI61127.1"
                     /translation="MKPQATIALYLIIAVLLLAAGCTGVDHREQDAAIVVAVTIAPEQ
                     EFVERVGGDHVRVVLLVPPGADPHTYEPPPGILADVAGADMYAAVGSGVEFELSWKDK
                     IAALNPGMLVVDCSRGVDLISTDPHIWVSPRNAKVMVENICRGLIEVDPENATDYRRN
                     ADAYREELDALDAEIADALARSGVTKIMVYHASWAYFARDYGLVEVPIESEGKEPSPQ
                     RIEHLVRQAEEEGIRVIFASPEHSTRSAEVIAKEVNGTVVQVSPLAGDYLANMRRVAL
                     AFAESGGP"
     gene            complement(17536..18000)
                     /locus_tag="PLI76_03505"
     CDS             complement(17536..18000)
                     /locus_tag="PLI76_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOI61128.1"
                     /translation="MQEITGHELSSKKAEYLKYIYMQGDVVRTTEIAAHFSVAPSTAT
                     KTLADIAKAGYIEQLPYHGARLTARGIDYARFLIRRHRIVALVLSRYGLRPDEACTEA
                     KKIEQCISKDLTDRICTSLGHPMMSGCGEIEHDCFCCPRTSREEDNLESGQT"
     gene            complement(18044..>18823)
                     /locus_tag="PLI76_03510"
     CDS             complement(18044..>18823)
                     /locus_tag="PLI76_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOI61129.1"
                     /translation="EDILDAVRHLVASGVYEIQLTGQDVAAWGLDRGESLPELLSAIT
                     DIPGRFAVRPGMMHPASVLGILKPLIDTYASDKIFGFLHIPVQSGSDAVLERMQRGYR
                     AADVIGIVDAFRERYPDMMISSDFIAGFPGETDEEFSQTLALLRRAAFVKVNVTRYSR
                     RPGTPAAALKDLPERIRKDRSRALLAEANRIYDRYNERWMGRETPVVATEKNAPETTV
                     CRNPCYLNVVVREDLPFGFSGRAVITENRRHYVVGELLQDI"
BASE COUNT         3172 a         6238 c         6178 g         3235 t
ORIGIN      
        1 cgacgaaccg gtagccgcgt tcgagcgtcc cgatgcactc ggtgaggagg gcgttcggga
       61 agcagtagca ccccgacccc tccggccggc ccatggcgag gaggtcgtag ccctccgcct
      121 ccgcgagagc ccggcggaac cggtatttga cgtagcccgt cctgtccatg cccgggggta
      181 tggcccgggt gaaggcttcc tcccgcatgc ccccgagcgt ctccgtgagc gcgatcccga
      241 gcgcctcgtg gaggtttgcg ttcggatcgg cgtccacggc aaggattggc cgctcgccgg
      301 ccgcgataag cgcatcgatc aggagcgccg cgatcgtcgt cttcccggta ccgcctttgc
      361 cggatatgac gatggtgcag ggccgggtca tggctcctca ccccggaggc ggcgggagag
      421 cgacgcggtt gctgccatga tctcgatggc ctcctcctcg ttagcgttcc ggatcccgca
      481 ggtcggcgtg atcagggatt ggcggtcgaa gagatcgggg tcgatcgtct ccgtcgcccg
      541 ggcgcggatc tcttcgaagc gggagaagaa cgcgtcgggc ttctccgccg caaaggcggc
      601 gtggtccgcg gggacgatac cccaggccac cacgcctccc ctctccatgt agcgggcgat
      661 ctcgtcggca tacagcagaa actcgcgggc gtaggtccag gcgtcgacgg agaccacggc
      721 cggcgcaaga ccgaggacga acccccagtc ggtgttggcg cagcagtgga tcccgagccc
      781 cccgtcgagg agggcggcga tatcctcgaa ggcggacgcc gccaccccgg catccaccgg
      841 gacgaccgac gagccgagcg acgcaaggta gggctcgctc agcaccacaa gggtcgcgcg
      901 tgcgcccatc cgctcccgca tcgccgtctc gcaccaccgg gcccgcaggc cgaggagttt
      961 tccgagcagg tcggcatact ccgggtcgta gaggatgggc cggcgcgcct cgtccaccac
     1021 ctgcatgccg aaggtcaccg ggccggtcac ctggcacttg acgagcagtg cgtccgagag
     1081 gtcgcggccc atcatggcgt ggaaccccga cccgtaagcc ttgccgaccc ggtagggctc
     1141 cgcgttccgt tcaaggtagt cgagcaggac ctgctccatc gcctcagagg gatcggtgct
     1201 ccggtcgatc acgagcctgc cgtcccggac caccccgccg gggagatgtt cggcatcggc
     1261 aaagacgatc gcctcgagaa acccccggtt cggcagggtg ggtacataag ggatccgggg
     1321 gaaggccgcg agaaccgccc ggcacgcctc aatcgggtcg cggtggggga gcgcccccac
     1381 gccggtcgcg aggagctccg gcggcggtat catgcctgcc acgacctcac cccctgtctc
     1441 gcccggcacg cctcaagtgt ctgctgcacg tgcgggaaga gcgtaaagtc ggtgtgcggc
     1501 aaaaagcagc tcgatgtgta ccggtccatg aaggacggct cggcgttcag ctcgatgtag
     1561 gtgatcttcc gggcaatcgc gtcgagctcc tttcgccgct tgcggtcgag cagggcgatg
     1621 ttcgcaccgg tgatcgcccc gttcccgatg ttctccacac gctccaggga tatctcgggt
     1681 atcaacccga tggtgatggc gttctcctta ttgaggtagt tcccgaacgc ccctgcaaaa
     1741 cagaccctgc cgatatcccc gggcataagg cccgccgcgt cgaggagcgt catgcaggcc
     1801 ccgtggatcg ctgctttgct catgatcagg ttcctgatgt cgttttcggt gatgacgata
     1861 tccttcccga gcgacgtctc ctttttccag accaccacga actccgggta gtactgccct
     1921 ttccggaccc gcggatggcc gatctccagg ttgatccgtc ccgtccggtc gatgacgcag
     1981 gcccggaaga gctccgcgag caggtcgatg aggcccgagc cgcatatccc ccgcggccgt
     2041 gcaccgttga tcgtcgagca ggtcggtgcg agcgtctcgg cgtcgatccg ggcccgctcg
     2101 atcgcaccgg ggtttgcccg catgccaaag agcacctccc cgccctccag ggccggccct
     2161 gccgcgcccg cacaggagag catccagtcg ctgttgccga gcaccgcctc gaagttcgtg
     2221 ccgatgtcca ccatgagcgt gggttcttcg cgctccgcca tcccggttgc caggatatcg
     2281 gcgatgatat cgccgccgat gaagttcgag accgccggga aggtgtagac ctcggcgttc
     2341 ttgtgggccg cgatcccgct ctgcccggca cccgtcgaga gcatccgccg cacgaccggt
     2401 atgtaaggct cggcgatcat gtaggcaggg tcgatcccga ggaagaggtg ggtcatcacc
     2461 gtgttcccgg cgatcatcac ctcaaagatc tcctcgcggt cgatccccgc ggcatcggcg
     2521 gcggtcgtca gggcctcgtt gatgctctcc gccgcaagct gctgcagccg ggaaaggccg
     2581 cttttgcggg agaagttcac ccgcgagagg atgtcctcgc cgcaggatat ctggcggttg
     2641 tagttcgatg ccgtagcgag gatccgcccg tcgacgagac tccagaggta gaccacgacc
     2701 gtggtggtcc cgaggtccac cgccgccccg tagagcctcc ccgtggtgtt gccttcgtcg
     2761 acgctgctca agcggtagcc gacgggcgcc agggcgaccg tggcggtcgc gtgccacccg
     2821 ctccggcgga gcaccagcgg gagataacag aggacgtcga gcggtgcgta gatactggtc
     2881 gggggcgggc cgccctgcag ctcgatccct tccagcacac gggtgagatc gggggtcgtg
     2941 tcgtccaggg acggcggctc gaggcggacc tcgtacttcc ggacggcagg gttgaacggg
     3001 atcccttcca ggccggggac ttccaccagg atcttctgct cctggatgag cgactcggcc
     3061 gggacattca cctggatgtc gccgacgacg gtcgcctggc aggcaagaca cgccccgcgg
     3121 gcgatctcgt cgtccgagaa gaacctgcgg tatttctcgc ggtcgaacgt taccgtgccc
     3181 tctttgatga agacaaggca cttcccgcac ttcccctgcc cgccgcagac cacgttcatg
     3241 gagaggccgg ccgcgcgcgc cgcgtcgagg agagtcgttc cctccggcac ggtcaccttc
     3301 cggtagcccg gaaggaacgt cacggtcggc atcacgtctt ccactccgtt tccaggtatc
     3361 cggggagatc ggccgcatca cgagggccga ccagcacttt ccagccggtc gcgtcctcga
     3421 tctttccgga gaggggggcg gcgtagccgg gaatgacgag cagccggtgg tctaccagat
     3481 ccccgacgcc gaactccttg atcgagtcgg cgacgagggt ctccgtcaat ttcccgccgg
     3541 cgacggccgt gaggaccgag agcccctcgg tatccgcgat gaagaggtag caggggattc
     3601 gagacgcctc gagataccca agcaacgtga agtaggtaag cgagaagttc accgtgagga
     3661 ggaccggggc gttacgcccg ggctcgttga tcgggtagat gcccggcgtc acctgaatgg
     3721 gtttctgcgg gtcggtgtat atgttctgcc gcagggtgag cgccgcctcc acggaggcgg
     3781 gggagagcgg ggggacgacg atgatccccg cgtacttcag tatgcccgcc gcgatggccg
     3841 catcggcatc cccgaaggcg ccggcgtcga tggagagggg gtagccgagc gcgggttcgg
     3901 tccgcccgat ggcagccttc cggatatccg tcgcaatggc gacgaagccg gcaagcgacg
     3961 acggcgcagg gtcgagcacg atatcgggga caccggcgtc tgcacacttc ccggcgaggg
     4021 cggcgagttc ggcaggatcg gcggcccgga cggcaagggg gcacccgtaa cgcttcgcga
     4081 gggcggcaag ggcttcatgg ttctcctccg tcgcggcgtg gatgagcggc cgatagtgcc
     4141 ccgcgaccgc gagggccgcc tcaagggccc cggggtcgtc ggcgatcagc gcgagaggga
     4201 ggctgtttcc acccccggcg agcgtcacga cctctgcaaa ccgttcgggg aggttgctct
     4261 cgtacttcac cgcgaccccg ttcaggacga gatcctggcc cacccggtgg acgaccgtct
     4321 cttcgaccat ctccacgacg gagcgcacct cgtcgtccgg ccaggcatcg gagacctgca
     4381 ccacgatccc cggcgggtgg tagaaggtct tctcgtgccg gaagaggacg aactcttcgc
     4441 cgaccgcaac cgaccgctcg cctgtgccga tggtgacgag ccggaccggc ggccgggtca
     4501 ccccgccgag gagggtcctt gcttcggcct cgaggtcggg gcaggcctcg acatccgcct
     4561 tgccgacggc gagcttcatc gcaaaggcaa ggcacgtggg gtagccgcac ttcttgcagt
     4621 tcgtcttcgg gaggtgcttg tatatatcga gcgctttcat cgccatgttt cagccctcca
     4681 gggccgtttt gagccttgcc acggtcaggg ggtggcggac gcagacgatc gacgcaccgg
     4741 cgacgacggc ggcgtaggcg gtgtagaact cccaggcaac cgccatcgcc tcctgatccg
     4801 ccggtgcagc ctcccgtact tcccggatcg agagggtgtc ggtgggggcg ctgatcatcg
     4861 gcatcgccag gtccgcatcg ccggcgaggg cggcgagccg gatccgctcc atcaccgagt
     4921 aggtgtactc gaacccgtag ccgagcgctc ccgtgtaggg gtcgatcacg atccggtcct
     4981 gcggcacgcc ggtctcgcgg aggaggatgt tcagttgctt cgccaggttg acgtcgatcg
     5041 gcgactgggc gatcaccgca tggccgtagg cgagcgccgc cgcggcgacg ctccggtagc
     5101 ggtcggcctc cgcggtcccg aggaggaggc gttcgccctc tcccgcctcc ccagagcggc
     5161 ggaagacctc gctgtcgagt tccggttcgt tgctcccctc gacgatcagg ggccgggccg
     5221 tcgccgcgag cacctcctcc gtcgtcgcgg cgatcgcgcc gaacgcatca aacccgcgct
     5281 gtttcgtgct cgtgaagttc agccgcacca ggtccgcacc gtaggtgcgc tccgcggccc
     5341 gtgcccactc agccgtgtcg ttcgcaaggt cgcccacgta gtcgcggacg accggcggcc
     5401 agaactgcgg gtcgtcgcag atctcaagcg ccaccagcag tcgctccggt ctcttcccgg
     5461 agaacgggag atcccgttcg cccccgatcg cgtaggagac ctcccgggtc cccccttcgg
     5521 cccgcgtggc gccgaggcgc acttcccgga tcgccaatcg ctcatcattg ctctccatca
     5581 cgcaccatcc ctcctatata agcgacctca tcgtgagcgc ggggtgtttg acgcgctcga
     5641 ggaagaggac gagatcctcg atcgtcggcg ccgagacctc atcggcgatc tttgcaaaga
     5701 ggtccggcac cccctgtgcg gcaagcctct cccgcagccg gtcgccgagt tcctctttca
     5761 gggaagacgg catccagacc actcgcatga tcccgttctc ggccgccaga aacctgtcgc
     5821 tcaggatgta gttcttcgat atgccgatga accccggcgt ctgcgcgccg cctccgatcg
     5881 tccccgcaag cgtcgagaag gtcatcccga gcggcgtctc cccggcaaag tcgcggctga
     5941 cgacgaggat accgttcacc tccggcacga tggccgcgat gcactcgaag cacccgcagg
     6001 cggtcatcgg gttctcgatc atgctgtaga gggagacccg gtcgacctca ccgtgggatg
     6061 ccttcttgac gaaccggttc acccccacga actcaccgta gagggcgtcg accatctccc
     6121 ccttcgggac cggctggttt gccccggcgg gggctatatc gtgggcaacc cgggcgtcga
     6181 gccaggtgat cgccccgcag agcgccggcc gctccggcgt gatgacgcag acgtggttcg
     6241 gggcaaacgt ctggcagagg gtgcaggagt agaacgtctc cacatcctcg tccttcatgc
     6301 cgctgatccg ctcgtcgcgc cgcgcgtaga tctcccgcgc ctccacaagc atcctctcga
     6361 cctgctccct atcggtgacg agcgtcacct ggaccgcgtc gatgtgatcc gggaactccg
     6421 ccttgagttt gtgggcaagc agcgtcccga tatcccggat cctgacgcct tttgccaccg
     6481 cttcctttga gagcctgacc cagatgaggt cccgctgcgc aacgtgccag gacccctcgc
     6541 cgtagttgac gaaggtatgg atccgccgct cgagcaccgg ttcgtagtcc gctctcatcc
     6601 tcgcgccggc gacgtccacc aggatcgcga gcgggagggc ggcgcctgcc gggacggcgt
     6661 cgacctccgg tccgacgacg gtgaccttcc cgtccttcac ctcctcgcgg ggccgcatcc
     6721 ggaggagctc gaaggcaggg gtcctcccgc cgccgaactc cacgtacatc tcctctttgc
     6781 ggatgctctt cccctcgaac gcgggggagc aggccatggg tatcgggatc gccgtgaccg
     6841 tgacccggat cccccggagg tccatgccgg tccggaccgc cgttgcggga gcgaccggta
     6901 tccactcccc tccctcgtag cccttgccgg ccgcgaggat cggtatcccg agcacccgca
     6961 gggcgtcgac gaacgcggcc tcttcgtcgg tgaggcccgg aaatgcgatc acgaacgcct
     7021 tcgcccgttc ggcggcgtag ccgagcaggg agtgaacgtc tcccggctgc acccccccaa
     7081 acatcatcgc gacccgggcg aggatgtcgg cgaagtggat gccgtgagcg ctctctggac
     7141 cgagcggcac caggcggtaa tccagcccca ggcggacacc ggcgccggag agcgcctcca
     7201 cgacaccgcc ggcgaggaag gtgagcaggt acttctcctg catctcccgg catatggccg
     7261 cggcggcatc agggctctcc ggcgtcccga cgaggagacc aaggcccgtg acgctcccgt
     7321 cgaccagggt gtagccgagc ctccggagga ccgcatccgc tataaagccg gtatacgggc
     7381 ttgccacgcc gccttcgccg gccatcaggc attcgcaggc gacgaggaga ttgttgccgg
     7441 actgccggta tgcctcgcgc gctccgtttg cgtcccgcac ggctatgccg gtgacggcgt
     7501 aggccacggg gagggcgtat gccgtctccg ggtaggcgag tttcccggcg agtgcctgca
     7561 tcctcgcgtc gaggccggtg cgaccgtgtt ctatcgccgt aacaagagaa tccgtgagtc
     7621 tgtccttcat gatgcgttca acagatggta ctcttcgcac ggcataaaac attgtctccg
     7681 tgcaggacgg cgccaatcgc ggtctttggg gctctccggc cggtgatgcc cgtccgggat
     7741 cacgggcgga tctcttcggc acacgccgcc aggaaggggt ctgcacctcc gtggaaaggc
     7801 cggtgcctcc gtgcaggggg gcacgctcgg tgagatccat ggctgcccat ctcccggata
     7861 tctcaccggg ataaaacatc actgccggtg gggccggact ccggcgtaat gggggggata
     7921 tcccggttac gggtgggcga tggcgatccc gcccgccctt cgccggtcag aaccagccct
     7981 ttctcttgaa gtagaagagc atgatgcccg cgacgaccgc catcagggcg agggtcacgg
     8041 ggtatcccca ggaatacccg agttcgggca tatacgcaaa gttcatgccg tagaccccgg
     8101 cgatgaaggt gagcggtatg aatatggtgg cgatgatcgt gagaaccttc atgatctcgt
     8161 tcatccggct gctctggctt gagaggtaga cgtcgagggt cccggatgcc gtgtcccggt
     8221 aggtctcgac ggtctcggca acctggatgg cgtggtcgta gacgtcgcgg aggtagacga
     8281 gcgtcgatgt gcggatcagg gacgaatcgc cccgctcgag ttccgccacc acgtcgcgga
     8341 gcggccagac cgaccgccgg agagcgatca gcgaccgttt cagggcgtag atcgcctgca
     8401 gggtctcgtg gtcgggatcc gccaccacct cctcctcgac cgcctcgatc cgctccccga
     8461 actcctcgat cacggtaaaa tacccatcga cgatcgcgtc cagcagcgag tagaagaggt
     8521 agtccgctcc ttcgttccgg atccgcccgg ccccggtgcg caggtgttcg cggatgcggg
     8581 aaaagacgtc gccgggccgt tcctggaacg atatgacata tcctgtgccg aggacaaggc
     8641 tcatctgctt gctctggaac gcacccgcgc cgtcggggga gagcatccgg actgccacgt
     8701 agagataatt gccgtaatcc tcgatcttcg ggcgctggcg ggtgttggcg atatcctcga
     8761 gcgtcagcgg atggattccg gcgacgtctc cgacagcctc gatgatcccg gcgtcgtgga
     8821 caccgtcgac gtcgatccag gtgaccgtcg ggcgggcgat gagtgcccgg aggtcttccg
     8881 gccggcaggt tcgctcatcg acccgtgacc cgtcgtagtc gatgaccgtg atggtgaccg
     8941 aatgggcggg tgtatccccg gtggatatga gcatcccctg cccgcctctc atccggttct
     9001 gctctgcgcg ttcatcgtgc cggctcatcg gacctgcgaa ctccgggtca aaaagagaag
     9061 ggttgccggg gcttatttcc tctctgccgc ctcgtcctcg cccttcgcct tcgctgcggc
     9121 gcggccgatc aggacgaccg cgatcaccgc gatgatcgtc acgacaacgg catacacaaa
     9181 catcgcaacg agcgtgctct gttcaccgaa tatgagcttg aagagctcct gaatggcgcc
     9241 gttccacgcg agggcggcga ccaggccgaa tgcggccgta acgagggctg ctatcttctc
     9301 gatgacctca gtcttgaagg acatgtggtt tcctcaaccg ggaagtgtac ggtaaacgat
     9361 ataatacttt cgcatttcgg ctttttccgg ggtttttcag gcaatagggc ctctattggg
     9421 agtccaaagg gcaaacgagc ggacatatgc gcgatgggaa gcgttcccgg cgggatcgcg
     9481 gggatcaggt ccccgcccgc cggcgcacct ttcacattca ccccgcacac ccaaactaca
     9541 ttgacgtcgg ccgccaacct gtatgtgatg gataccctcg acgactggtt cccgtaccgg
     9601 gagtaccgac cccaccagcg ggagatgctc gaagcagccg catctgtcgc gcgcgacggc
     9661 ggtatcgcca tgatcgatgc gccgaccggg agcggcaagt cgagcgtgat ctctgcgctc
     9721 ctcgcggaga accgggggcg caaagtgctc gtcgccgtcc gcaccatcag ccagctcgcc
     9781 acgttcatgc gcgagctcga gctcatccgg aagaaacgcg gcggcctgaa gttcgcctac
     9841 ctcatcggca agtcgagcat gtgcccgctc ggcggcgagg gggacgtcta ccggcggtgc
     9901 gagggcgtca aggccttctc gacggcgctg atgcgggagc gggcgcagaa gggatcgctc
     9961 gtcccggcaa acgaccgcca catcaagcag cagatccgga agatggaccc ggaccacccg
    10021 ctcatatgcc cctacttcat ccgcggcaaa tcctttgtcg agccgggaga ctcagggctg
    10081 aagatgatac cctcggcggc cctccgcacc cgggcggagc gggtgagcac cgaactgatc
    10141 tggcccgacc ggctcgccgg gttctgcggc gacatctgcc cctacgacac gatgatgcac
    10201 gccgcgcgcg acgccgacgt gatcctcgtg aacttctacc acctctttga cgacaccatc
    10261 cgcgagcagc tctacctctc gctcggcatc gaggggtctg atgccctgct gctcatcgac
    10321 gaggcccaca actgcggcga cgtcgtccag agcatcgaga gcgtgacgat cgaggagcgc
    10381 gacatcgtgc aggccggcca cgaacttgcc gggcggcggc ggtcgcagca gtcggcggac
    10441 gccgtcaccc agatcctgcc gcagatcacc cggttcatgg aagcgctcaa ggcgtcgcac
    10501 gaggtcgagg actggttcga cccggccata ttccagcgga tgatccttgc cggcacgctc
    10561 taccggaacg tggaaggggt cgtcgacgat ctcctcaaga tcagcgaggg gatccgcgag
    10621 aagaacatgc aggcgggcga gttccgggag agcgccatcg agcggctgac cgagttcttc
    10681 taccggatat ttcggtctgc agacgacccg gcctacctga cggtctaccg gaaggcagac
    10741 gacggatcgg tggcgctcga ggtgaggaac atcgatccga gcaccaaact ccaggagatc
    10801 gcgcaggcgc atgcatgctc tgtcctgata tccgggacgc tctcccccat cgagagttac
    10861 cggcgctact acttcggcga ccttcccgtc accacgatat cgctcccgaa cgccttcccc
    10921 cccgagaacc gccgcatatt ctgcgccgac gacatcacca ccgcctactc gatgcgccgg
    10981 gacaaagaga acctcgcgcg gaccgaggac tacatcgcca cgtttgccgc cctcccgggg
    11041 aacctggccg tctacttccc ctcctacgac ctcctcaaca cgttcgcgga gcggtgcgcc
    11101 ccccggataa ggaagaaaca gatctacgtc gagcccaaag acaccgccgc cgcttccgtc
    11161 atgctccggg agttcatggc cctcccgggg acgggccggt ttggcgtcct ctttgcggtc
    11221 tctggcggca agtggagcga gggcctcgac taccggggcg agatgctcgc gggcgcgctc
    11281 gtcatcggcc tcccgcttgc gccgttcaac cgcgtccgcc ggatggtgat cgactacttc
    11341 cgcatgaagt tcggggcgga gggcgagttc atcagttaca ccctcccggc gatcaaccgc
    11401 gctctccagg cgctcggccg ggtgctccgg acacccgaag accggggggt gctcgtcctt
    11461 ggagaacggc ggttcctgga atcccgggtc cacgccggtc tgccgccgtg gatgcaggag
    11521 gagatgacga cctgcaccat cgacctcttc aagaaggagg ccggccgatg gcggttctga
    11581 ccggatcgtg ccggttcggc ctctggcacg tccacgtcgc gtggcaggac gatctcgtct
    11641 accgggtgcg ttttgcgcgt accgggcttg aggggccggt cccggaggag atcctgcttt
    11701 actgtgccgg gcgccgggcg gacctctcct ccctccggag tgttgcgacc gggggaagtt
    11761 cgacgttcgc cgcgatctat cgcgcggttc gcgacatccc ctccggagag accgccacct
    11821 acggcgatat cgcccaaagg gtcgggaccg cgccccgggc ggtcgggtcg gcgatggcta
    11881 agaacccgac cccgatcgtg gtgccctgcc atcgggtcgt cgcaaagacc ggtataggcg
    11941 ggttcacccc cgatccggcc atcaaggagg cgctgctcgc catggagcgc cgggagaaag
    12001 ggagaagtta acaggtaact gcatcaatat agtagtgaca tgaagatcgt ggtcctgggt
    12061 gcgggagcgg tcggcctgac catcgccgca aagctctccc gagtcgcaga tgtccatgcc
    12121 gttgcgaggg cgcggcacgc ggacgcggtg cgggagcggg gtctcttttt gaccggcata
    12181 tggggagagg gcacctaccg cttcagttgc tctgaagacc ttccccccgc gtggcgggct
    12241 gccgactact tcatcatcac cgccaaatcc accgatacgg aggcggtctg ccgccagttt
    12301 gcggatgcaa tccggggaca ggaggtggtg agcctccaga acggcatcgg gaacgaggag
    12361 gtcatcgggc ggttcaccga ccgggtcatc ggggccatga tcatcaccgg gtttgagtgg
    12421 cggggcgatg cggcggttca cgtctcggtg gaggcggcgc cgatgaggct tggcaggttc
    12481 ccgtccggca cggacgaggc ggtcgtccgc cttgcctctc tcttttcgga ggccggcatc
    12541 cgggcggaag cgaccggtgc gatcaggagc gacctctggt caaaaaccct ctacaactgt
    12601 gcgctaaacc cgctcggggc catcatgaac gttccctacg gggcgcttgc cgatccgcac
    12661 gcctgggcgg tcgtcaccgg gatcgtccgg gaggcctatt tggtggtgga ggccgagggc
    12721 atcgccctcc cccgggcaac gccggaggcg tacctggagt atctccggac cgtccagctc
    12781 cctgcgacgg ccgcccacca ctcctcgatg ctccaggacc tcgcccgcgg gcaaaagacc
    12841 gagatcgagt tcatgaacgg cgccgtggcg gcgctcgccc ggcggcacgg tctcgatgcg
    12901 ccctataacg cctgcatcgc cgaactgatc cgcttccggg agcggctcta gcgcagggtg
    12961 agcatgcgat cggcaattat cctcgtgggc ggagcggcac ggcgtgccgg agggcgggag
    13021 aagtacttct tcaccttcca gggcaagacc ttcatcgacc gcctcatcga taccctgcgg
    13081 gaggtggtgg acgagatcgt cgtggtcgcg cgggacccgg agcagtgcga acggttcatc
    13141 catctcgacg acgtcaggtg cataccggat atcaggaggg atctcgggcc catcggcggc
    13201 ctgcacgcgg ggacgcttgc ggcgcacggc gagtacatat tcgttgccgc ctgcgacatg
    13261 ccctgcatca accccggggt ggtgaggatg ctctttgacg ccgctctcgg gtacgacgcc
    13321 gccataccga gctggaacgc cgatatgctc gaaccgctcc atgcggtcta ctggaggagc
    13381 gcgctgctcg cctacctgga ggagcacgag tccctctcgc tccgcccgat gatccggagc
    13441 ctgaacaccc gttacgtgag cgtcgagacg atccgcgaga tcgaccccga cctcctgacg
    13501 ttcacgaaca tcaacaaggt cgaggacctg cagttgatcg acccgtcgac ggagcacggg
    13561 caggacgatg cgtgcaggtc cgggctggaa ccgtcctgat ccggcccgga ccgcgccgtc
    13621 tcccggttct ctgcggcccg aggcgatgac ctccccggcc cccggcgcca aatgtcccgc
    13681 gggatacccc cgtgccccgg ggctccgccg cgccgcctgt cgtgggattg cacgggtgcc
    13741 ggtctgccgt ccggggaggg cgggcctcct cccgaccgaa aggcttatac cggttatctg
    13801 tgtagtcagg gcctgatgtc atcgggggaa gtgacttcaa ccagatctac cggagggggg
    13861 aggctcgact ccaggaccgt gccatccacg cgctccgact cgaaaagccg ccggccgggt
    13921 gccgcccggc tccctgtgct gcttaccata ttctgctgtc ttgccgctct cctgtgcggg
    13981 accgctaccg ccggtaaggg agactacgcc cctctctggg agaagggggc cgacgatgtc
    14041 gtcagtaccg tagcgatctc ccacgacggt tctctgatcg ctgccggcgc gggctcgacg
    14101 gtctccctcc tggaccggga gggtgatatc ctctgggaga ccgcggtcgg ctcccggata
    14161 agcgacgtcg ccatatcgcc cgaaggctca cgcatcggcg ttgccgcgga taaactctac
    14221 ctctttgacg gtgacgggaa cctgctctgg gccgagaaga cgacgtatat ctaccggggc
    14281 gtggccctct cgtcagaagg catctccatt gccgctgcat gcgacaacgg cgctctattc
    14341 gtctttgacc aggataagaa tatactctgg gactacgata tgggcaccga cggttacggc
    14401 gtcgccatat cggagaacgg ccggaggatc gcgctcgggt gcgacaacca tggggtctac
    14461 tacctcaaca gcggcgaggg ggagtcctgg agttacggga ccggtaactg cgtaaagggc
    14521 atcgccctca ctcccgacgg tcggtttgtg gccgccggat ctcttgaccg gtgcgtctac
    14581 ctctccacgg cggaggggga gcacctctgg aagtatccca ccacagaggc cgtccttgca
    14641 acggccctga ccgaagaggc gcgagaggtc ctcgccgcat cgggcaggac gatctacgtc
    14701 ctcgatcagt cgggggccga acgccagaag atcaccctcg gcggtcgcgt ggagtcgatc
    14761 gccgtgaccc ccgacggttc gttccttgcc gccgggggcg gcgacggcga tcggtccgtc
    14821 cacctcttca cgaaggatgc ggccctgctc gagcgcatga atccggtgga gcctgcggtg
    14881 aacgagaccg atctcccgga ggagaacgtg accgtccccg cgacccttgc agacgagagc
    14941 gcggatacgg cggcagaggt gaatgcccgg gccgggggtg aggaggtctc cctcttctcg
    15001 acgatctcgg gctggctgga gaacatactc tccctgctct tcaagcccca ggcggacttc
    15061 atcgcctgac cgacgcggcg gccgcggact cgctccccct cccctccctg aggcgctcgg
    15121 ccccgagagc gcctgcgtac gcaagcgtgc tcacgagaac gatcgtcgcg cccgacggaa
    15181 catcgatgac gtaggagagg gcgatccccg cgacggtgaa gatgatgccg aggacgacgg
    15241 caaggagcat catggtccac acgcgtgcgg tgtagaggcg gctgatcgcc gccgggagcg
    15301 tgaggagcgc gatcacgagg atgatcccca ccacccgtat cagcatcacc accgtgagag
    15361 cgatgagcac caggaggagg agcgagagcc gttcgacggg aagattcatg accgccgcgt
    15421 aatccgggtc gaacgtgacc gcctggagct cccggtagag gacggcgacg acggcgacga
    15481 tgacggcggt gaggatcccc atcagcagga tatccccccg cggcacgagg aggatgttcc
    15541 caaagaggta ggagaagagg tcgggcgcaa accccggtgt caggtagaca aagaggatgc
    15601 cgatagccat cccggccgcc cagaccgcgc cgatgagggt atccatctgc tgccgggccc
    15661 ggagctggag cgtgcccaca ccgagcgccg ccgccacggt gaacccggcg gcgccgagga
    15721 gcgggtcgat gccgaggaag tagccaagac ctatcccccc gaaggccgca tgcgatatgc
    15781 cgccgctgac cgagaccatc cgccgcacca cgacgtaggt gccgatgatg ccgcaggcga
    15841 tactcgcgag tacgccggcg atgagggcgt ttctgaagaa ctccagtccg agcacatcca
    15901 acatgctcac tcctcccgcg agtgttcggg aagtacccgg tgcggcacac cgtgggcgat
    15961 caggtccacc gggcagcggt aggccgcctc gatcatgtcg ggcgtgatct cgtccgtgtc
    16021 gtgcgtgtag aggcgccggt tcaagcaggc aacccgggtc acgtgcccgg ggagcacccc
    16081 gatatcgtga gtcacgagga tgatcgccat ccggtcgcgg agcccgtcca ggatctcgta
    16141 gaactgcgtc tccgtcgggg catccacgta gacggtcggc tcgtcgagga ggagcgcctt
    16201 cgggtcgccg acgagcgccc gggctatgat cgcccgctgc tgctccccgc ccgagaggtt
    16261 ccggatctgc cggtcggcaa gatcggcgat ccccatcgta tcgagcgcct cctccgtccg
    16321 ggcacggtct tcggccccgt agcggcgcgg gcggcgggcg atgcgcccga ggcggccgga
    16381 gaggaccatc tctctgacgg tgatggggta gtcgaagtcg aacgaccgga actgggggac
    16441 gtacccgagg tcctttcgca tcgccggatc ggtgctgccg ccgatccgga cctccccccg
    16501 gcagggggcg aggaggccga ggatcacccg gaggagcgtc gtctttccgc cgccgttcgg
    16561 cccgatgatg gcgtagaact cgcctgaatg gatggcgagg ctcaccgcct caaggatggt
    16621 ccggccgcgc agcctgaccc agacgtcgtc gacctcgatc acgggagcgg tcatggtccg
    16681 ccgctctccg cgaatgccag ggcaacgcgc cgcatgttcg caaggtaatc ccccgcgagc
    16741 gggcttacct gcaccacggt gccgttgacc tcttttgcga tcacctcggc gctccgcgtg
    16801 gagtgctcgg gcgacgcgaa gatcacccgt atcccctcct cctctgcctg cctgacgagg
    16861 tgctctatcc tctgcgggga gggctctttt ccctcgctct cgataggaac ctcgacaagg
    16921 ccgtagtccc gggcgaagta ggcccacgac gcgtggtaga ccatgatctt cgtcacgccg
    16981 gaccgggcga gcgcatcggc gatctccgca tccagggcgt cgagctcctc ccggtacgcg
    17041 tcggcgttcc ggcggtaatc ggtggcgttc tccgggtcga cctcgatgag cccccggcat
    17101 atgttctcga ccatcacctt cgcgttccgt ggcgagaccc atatgtgcgg atcggtcgat
    17161 atcaggtcga cgccgcgcga gcagtcgacg acgagcatac cggggttcag ggcggcgatc
    17221 ttatccttcc aggagagttc gaactctacc cccgatccca ccgcggcata catatccgcc
    17281 cccgcgacgt cggcaaggat acccggcggc ggttcgtagg tgtgggggtc ggcccccggc
    17341 ggcaccagga ggacgacgcg gacgtggtcg cctcccaccc gctccacgaa ctcctgttcg
    17401 ggggctatgg tgaccgcgac gacgatcgct gcatcctgct cgcggtggtc gactccggtg
    17461 cagcccgccg cgagcaggag aactgcaatg atcagataga gcgcgatagt agcctgtggt
    17521 ttcatcctct ccacctcagg tttggcccga ctctaaatta tcttcctccc ggctcgttct
    17581 cgggcagcag aagcagtcgt gctcgatctc gccgcagccg ctcatcatcg ggtgcccaag
    17641 cgacgtgcat atcctgtccg tgaggtcttt tgagatgcac tgctcgatct tctttgcctc
    17701 ggtgcaggcc tcgtcgggcc tgaggccgta gcggctgagg acgagagcaa cgatccggtg
    17761 gcggcggatc aggaaccggg cgtagtcgat gccgcgggcc gtgagacgcg ccccgtggta
    17821 gggcaactgc tcgatgtagc ccgctttcgc gatatccgcg agcgtctttg tcgccgtcga
    17881 gggcgcgacg gagaaatgag cggcgatctc ggtcgtcctc acgacatcgc cctgcatgta
    17941 gatgtacttg aggtattcgg ccttctttga agagagttcg tgcccggtga tctcctgcat
    18001 gaagagtgga aggatctcta tggaagaaaa gttttcggtg tggctaaata tcctgcagga
    18061 gttcgccgac gacgtagtgg cgccggttct ccgtgatcac cgccctcccg gagaacccga
    18121 acggcaggtc ctctctgacg acgacgttga ggtagcaggg gttgcggcag accgtcgttt
    18181 cgggcgcgtt cttctcggtc gcgacgaccg gtgtctcccg gcccatccag cgctcgttgt
    18241 agcggtcgta tatccggttc gcttcggcaa gcagcgcccg ggaccggtct tttcgtatcc
    18301 gctcggggag gtctttgagg gcggcggcgg gcgtgccggg ccgccgggag tagcgggtga
    18361 cgttcacctt cacaaacgcc gcccgccgca ggagggcaag cgtctgcgaa aactcttcat
    18421 ccgtctcccc cggaaacccg gcgatgaagt ctgaagatat catcatgtcc gggtaccgct
    18481 cccggaacgc atcgacgata ccgatgacgt ccgccgcgcg gtaaccgcgc tgcatccgct
    18541 cgaggacggc atcggagccc gactggacgg ggatgtggag gaacccaaag atcttgtcgc
    18601 tcgcataggt gtcgatgagg ggcttgagta tcccgagcac cgaggcgggg tgcatcatgc
    18661 ccggccggac ggcgaacctt cccggtatat ccgttatcgc cgagaggagt tcggggagcg
    18721 actcgccccg gtcgagcccc caggcggcca cgtcctgccc ggtcagctgg atctcataaa
    18781 ctccggatgc cacgaggtgc cggacggcat cgagtatgtc ctc
//
LOCUS       DAOSEE010000027        18002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1268_length_18002_cov_205.300997, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000027 DAOSEE010000000
VERSION     DAOSEE010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 18002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18002
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1268_length_18002_cov_205.300997"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..930
                     /locus_tag="PLI76_03515"
     CDS             175..930
                     /locus_tag="PLI76_03515"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HOI61130.1"
                     /translation="MRLKDKVVLVTGASSGIGLEIARLFAGEGASVVAVARREDKLAA
                     LVDQITAAGGKAVSVAGDVTREEDIRNAIRAAVETFGKLDVVVNNAGLMDRMEPVAEL
                     DDDTWNAVVDVNLTGPMRLFRGAVPEMEKAGGGAFVTIASVGGIRGARAGAAYTASKH
                     GVVGLAMNVAYAYAKKGIRSNVIAPGGVSTDILTGKSLSREGTEVYMAGMATNPRMGE
                     PIEVARVALFLASDEASLVNGAVITADAGWTAY"
     gene            994..2283
                     /locus_tag="PLI76_03520"
     CDS             994..2283
                     /locus_tag="PLI76_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="condensation protein"
                     /protein_id="HOI61131.1"
                     /translation="MSPRYPAPAFDVFNVYFERIYDPTMHAVFTFDGGIDAGALRKAT
                     MRTVASDPYTRSRYAEVDGRPVWEEIAEEEWARAFVVLPAGEDTGMPPPPLDVRAGPQ
                     VRVGLYPRAGGDIVAVTCHHGFCDVSGTMALARDIFATYRGIVADPGYRPPPREPYDR
                     STDRILALSSDEEQKRALAEEEPFIDRWRFPAGRMGRGTPRIASRTLAPDRLERMKAF
                     GREHGATVNDVLIGAFFLALLDIRNDPADRGAPRSILTSADLRRRYPGCHGEGLPVNL
                     SVAYDVTLTVEEGAGLEDIIGRVTAITAHKKAGNPGLAAILFYEGIMAGGMPAVRAFF
                     DEMAGRYTSSGNKNPVFSNLGIFDPGDYLPIPGEDGRVLDLLDIQYLPCVCWPYGFLM
                     AASTFCGRLTVTTAYEEGPYSTATVERFLGCVDGYLP"
     gene            2341..2829
                     /locus_tag="PLI76_03525"
     CDS             2341..2829
                     /locus_tag="PLI76_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOI61132.1"
                     /translation="MDARRFSVTPVGTVHAGRGVFSIEIAEPFRPALTELSGFGHMLV
                     LWWCDGVDTVECRSETTCEKPYTRGPDTIGIFATRSPVRPNPIALSVAPVLGVDAAAG
                     IIRVAYIDADDGTPVLDIKPYLPCNERVRDAKVPAWASRWPQWYEENEGFDWAAEFTF
                     GD"
     gene            complement(2872..4329)
                     /locus_tag="PLI76_03530"
     CDS             complement(2872..4329)
                     /locus_tag="PLI76_03530"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HOI61133.1"
                     /translation="MTNKKRLTTAAGAPVPDNQNAVTAGPRGPMLMQDVWYQEKLAHF
                     NREVIPERRMHAKGSGAFGTFTVTHDITRFTRARLFSDIGKKTDVFMRFSTVAGERGA
                     ADAERDIRGFAVKFYTEEGNWDLVGNNTPVFFIRDPHKFPDLNRAVKRDPHTNMRSAT
                     NNWDFWTSLPESLHQVTITMSDRGIPLSYRHMHGFGSHTFSMINAENERVWVKFHLQT
                     RQGIKNLTDQEAEAIVAKDRESHQRDLFESIRKGDFPRWTMYIQVMTEEQAKAMPYNP
                     FDLTKVWYKGEFPLIEVGVLELNRNPENYFQDVEQAAFNPAAVVPGIGFSPDKMLQGR
                     LFAYGDAQRYRLGVNHHQIPVNAAKCVANPSHRDGQMRVNGNAGTTIGYEPNSFGEWQ
                     EQPEYREPFLALSGAGGSWNFREDDADYYTQPGKLFRLMSPGQQQVLFENTARAMGDA
                     PELIKVRHIANCLKADPAYGKGVADALGIPLDRVK"
     gene            4569..5435
                     /locus_tag="PLI76_03535"
     CDS             4569..5435
                     /locus_tag="PLI76_03535"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOI61134.1"
                     /translation="MTGIIAFSDLAWGTRERGLPGGGRVGIASFMRIVDEADPAIVIF
                     AGDAAYDRCSRSGLDETEVFVDLLRDIAAAGRHCIVVEGNNDNARGTYGRVREAAEAI
                     PFLHEVSGKVEAVHGIRFLGVPTGKERRMARSAEGPVDIVVAHAPLADRVWLFDLPAA
                     CIVTGHYGMMVAGIAGKAYVALDCSPASYAAIEWEGGWRRIAYVAGACRISLSPGEGV
                     AATGCDAEERRRLTGGPGELPYRAEIEALRQAKDEVAVLGREEVAGRLLAMGIKKTHI
                     ERYLGRQGRPEP"
     gene            5555..5983
                     /locus_tag="PLI76_03540"
     CDS             5555..5983
                     /locus_tag="PLI76_03540"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOI61135.1"
                     /translation="MDDLVRERILQKVEVLIDRITFIEHHLSDAVMNDRILRKALYKE
                     FQEAVVSATDICALIRRILLSSAQDDYTNLDYLVGEGIISRETGRDLKAANGLRNRLV
                     HEYDGINDHIAYGAMQRLMPSLRRFSGEVLTWLNANRTRS"
     gene            5953..6345
                     /locus_tag="PLI76_03545"
     CDS             5953..6345
                     /locus_tag="PLI76_03545"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOI61136.1"
                     /translation="MVECEQDEILNHFRDLLPSIRAILLFGSRVAGYAGPTSDIDICL
                     ILTGEADRTAVHDGMLIAPEHYDIVVYDEIPWYLRGGILERHRIIYAEDPDDLDFWLY
                     KQRRIWGDMKRRQQRASSEDLLKRIGYR"
     gene            6526..7239
                     /locus_tag="PLI76_03550"
     CDS             6526..7239
                     /locus_tag="PLI76_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOI61137.1"
                     /translation="MDPVIEAIRQEFAAHADPDRRKSSLRFFKEEIRCYGMKTATAVT
                     VAKKYWKKVRSREKQEIFGLCEELYRSGMMEEAFVVSQWAQMLGERFEPGDIAVFRHW
                     IDTYITNWAACDGLCNHAVGDFVVRYPEHIEELKRWAQSDNRWMRRAAAVSLIVPAKH
                     GEFLDEAIAIADLLLADTDDLVQKGYGWLLKEASREHTDEVFSYVMRNKRVMPRTALR
                     YAIELMPKDLKAEAMKKDW"
     gene            complement(7248..7676)
                     /locus_tag="PLI76_03555"
     CDS             complement(7248..7676)
                     /locus_tag="PLI76_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOI61138.1"
                     /translation="MDFSECVKFANENPVTYIATMDGDQPRVRAFVMWFADATGFYYH
                     TGTPKAVWRQLAKNPKVELCFYAPGDVNTMMRVAGAVEFLDDAALKERLVGDRPWLLQ
                     IGISGPADPKLAVFRVAHGEAYFWTMEHNMREADAPRVRF"
     assembly_gap    7845..7944
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8185..9342
                     /locus_tag="PLI76_03560"
     CDS             8185..9342
                     /locus_tag="PLI76_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04083 family peptide-modifying radical SAM
                     enzyme"
                     /protein_id="HOI61139.1"
                     /translation="MKSPFHVMIIPTLGCPSKCHYCWSSDERSPRMSIETVREITEWL
                     KGYQSNQITITFHGGEPLLAGAEFYRQALPILSEGLAHLTPTFALQSNLWLLTPDMAR
                     ILAEYRIPIGSSIDGPEEINDVQRGKGYFKKTMRGYDIARANGLEVRFICTFTNRSVE
                     RKEEIFDFFLQNGFPLKLHPALPSLRSENPKEWALEPEAYGELLVYLLDRYLENLGRI
                     EVMNINDICRGVFTRHGTVCTFVDCMGTTLAIGPDGSIYPCYRFVGMPEYVMGNVYDR
                     PGPEDLARSDAWRLMQEFREYVDRECKGCPHIKYCRGGCPYNAIAPTDGEIRGVDPHC
                     TAYRRIFDEITDRVNKELYDSTDMEMGDFGSRQRRRARPGIMSILQAMAER"
     gene            complement(9362..11677)
                     /locus_tag="PLI76_03565"
     CDS             complement(9362..11677)
                     /locus_tag="PLI76_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOI61140.1"
                     /translation="MHEPIPAYGSISALSAVLDELDEGLLVVGSDNRIACINKRLRHI
                     LGIDRDAAGGTDATRFVHQTLAPRIAEESCRRDLLEFFSGRQRSLDLSCTLLTSGGKS
                     NRVCCSCKTVGSGLFQGMRVARFEPVPREDSASPGWIGDLLRQDDDWYRFLVENLNEG
                     IGLIDREGIVVFANKRLADIIGCPIAETVGAPASRFTDEEGARCMEDYLQNLDTFPHE
                     IFEIDLIRRDGIRIHALMATTPVIDASGACRGFLAGVLDITPLKQMELQLEESKEKYR
                     SLVELSAEAILIYQDGIVTYINPAGVRLLGAQDPGEVIGTNVADIIHPGSRETVRSFA
                     ARNLQRGETPLVELPVVRLDGTMVFVEGRGTRAFFAGRPAVQVVMRDITHRKQAEERL
                     QASNRHLLLLNRIIGISAQAQTPDELLETALDQTLDLLGYDGGGIYLTEPGRSGVALR
                     YERGMPKRCLERAGELFRASYAEVCATGAPRYIEQDDSPVSRLLRDIGFTALACTPFT
                     VEPDVAGALLVGSRNRQSFPPEERALLEAVGREIGAGMLRGMLHQRLEAANREANLYL
                     DILTHDIKNADNVANIYADLLIDELEGEVVGHAQKLKDGIRKSIEITANVATIRKIHE
                     TRAGLEPVDLDAVIKSEIEHFPDLPICYDGLPLKVFADDLLPQVFTNLIGNTTKHGGS
                     KVNVAVAVEDLGEEVVVTVADTGPGIPDDMKETILYRFERENDKRGSQGLGLSICRML
                     TARYGGRIWIEDRVAGRPGEGAAFRFTLRKA"
     gene            12084..12299
                     /locus_tag="PLI76_03570"
     CDS             12084..12299
                     /locus_tag="PLI76_03570"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HOI61141.1"
                     /translation="MTLIRLLVFLAVPVYRDVNRPGVNGLLRFIPAFLILVIRRPGAG
                     DAAMDLLRQRCARGEIDEEEFQRERRG"
     gene            12301..14253
                     /locus_tag="PLI76_03575"
     CDS             12301..14253
                     /locus_tag="PLI76_03575"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0043682 - P-type divalent copper
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HOI61142.1"
                     /translation="MGHGEEAGRETHHHIFDDYRRRFIVSVILTVPVLLLSLPVGALP
                     IAFPGSAYLLLALATAVYLYGGWPFLSGFVHELRARMPGMMTLVAVAITVAYVYSSAV
                     VIGVVGGEGFYWELATLIDVMLLGHWVEMRSVLGASRALEELVRVMPSEAHRVRDGGT
                     DDVPVDRLVPGDRVLVRPGEKVPVDGVIVEGGTSVNEAMLTGESRPIEKRPGDTVIGG
                     AINGEGSIVVEVERTGAETYLARVIDLVRKAQESRSRTQDLADRAAFFLVAIALSVGA
                     VTFAVWFAIEGTGFAVERAATVMVIACPHALGLAVPLVVAVSTALAAHSGLLIRDRAA
                     FERAKDLEAVVFDKTGTLTTGTFGVTDVLAFGGAEADVVRAAASVEAHSEHPLARAVV
                     RSAEERGLSLLPVEGFRALPGKGVEGSVGGRRVSVVGPGYLAERGMAAGDERVEPLQR
                     QGKTVVFLIEGDRLTGALALADTIRPESGEAISRLKAMGIRCMMLTGDHHDVAAWVAG
                     ELSLDEFFAGVLPHEKAAKIREIRRRYRVAMVGDGINDAPALVEADLGIAIGAGTDVA
                     VESADIVLVRSDPRDAAAIIDLAKKTYRKMVENLLWATGYNAVAIPLAAGVLFSAGIV
                     LTPAAGAVLMSASTVIVAVNARMLRV"
     gene            complement(14265..17531)
                     /locus_tag="PLI76_03580"
     CDS             complement(14265..17531)
                     /locus_tag="PLI76_03580"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61143.1"
                     /translation="MVEKQTNPEEVNSLWWLLSVWAGSYYRDLMYCFSHQVGQSYTVI
                     RGLYYSLRLQFVLIAIAVLAFDCLSANLADKQMDVSPINLEWVSIDVLPAIMNVLLIW
                     FQSISLIKGVELFGVILLLFLAFSLARRANVASKIVTIDEFKDCRQKNHVRGLEENVP
                     NVAEDLHHRLAAELTRIRSLYENVAGSMDVTYGYRPEPPRITADYSTTSKNISQSNQP
                     LSIGPINLPQEIIRDIASRVVRGTRITGILFEEDDRLHLVAHRIDNEEPLSWTVVGPK
                     KAHGDGTSSEPALNDMIHELACRIFTSIYTDDATEWKAVQAFNRGLEEYLHSKAKPQG
                     RTIGLIRAEEKFIETLSEDSGYHNAYHNLGVIYREQKKWQAAERVFILSTHSRPKEAE
                     SYYTLALNYFDEAYDHLYEGDIEKRGSLERDDGIFRDYWSLCRVIELCDACLMLQPVC
                     PKAYELKGFAYGLRGYITEKRPDVINRPDPSGDLDYPGKLPPFESGGHDSLDHAIQCR
                     RIAVEQSWKLLCQNLLRPSQKNDRANPLVTVTEDRDRAYKCLNNMAYTLHLKSLKRKS
                     GNSGSEKSHSTIDRLSLWLLSQIQMRIYRQALALNPSKANPHLGIGGLHLMANRPDLA
                     IESFKRAISIDYTLPTAWMYLALAYNESIANIKRIQKPGAKHQVGYPDSRSAMPGIYR
                     QGRDHALIQFLNYIEELKPLHLYRIAEEFENRIECKDITLLIRRSLAFLEETRKCESS
                     KAIDLSPPEIPSSGIEHLRSIYDDWQKGESLRVQGINFLSHGEPGKADEMFIESLQSL
                     KTLYPERIRRNAIYASQAHAQLQKKDYPTSIKTTHDALLLDPGCSHSHLRRGQGLYGI
                     NDLDHCINEYRLALQCDPEQPEIYFRIGQVDLLRAKRSHNPEEKRELSEEASNNFKIA
                     LELRKGESKDIAKMYYWLGKSSLIMGSYNEARAYLETAESLSIAPIKVAFHLGEAYLG
                     MGLFETSENYFEQVRRHLDKSIRDSTISRDSWLQSEVEEFLEDDVCAGALLARWHIRN
                     ATLAIHTSQSGEEAEKHLEAVREIIGEISSTTTRRDLESEYLRARRWLLKQKEHLKPT
                     "
     gene            complement(17884..>18002)
                     /locus_tag="PLI76_03585"
     CDS             complement(17884..>18002)
                     /locus_tag="PLI76_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011021079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI61144.1"
                     /translation="AAVLDRILHHSTTLNIKGDSYRLKERRRQGIYSHTLPG"
BASE COUNT         3353 a         5247 c         5627 g         3675 t
ORIGIN      
        1 atgctgtatc ctgttcttcc tgatacttat ggtacctttt tgccaccatc tcaaatacct
       61 ttgtagcacg ccggcagcgc cgtcatcgct tcccggagca gaccggcggc catgtccgcc
      121 gcgtgccgaa acatttagga tgtcataccg cgctccccgg ataccgggga aaccatgaga
      181 ttgaaagata aagttgttct cgtcacggga gccagttcgg ggataggact ggagattgcc
      241 cggctctttg ccggggaagg cgcatccgtc gtggcagttg cacgaaggga ggataaactg
      301 gcggcgctcg tcgatcagat aaccgccgcc ggcgggaagg ccgtttcggt cgccggggat
      361 gttacccgcg aagaagatat ccggaatgcc atacgggcgg cagtagagac gtttggtaaa
      421 ctcgatgtcg tcgtcaacaa cgccgggttg atggacagga tggaaccggt cgccgagctc
      481 gatgacgata cgtggaacgc ggtcgtcgac gtcaacctga ccgggccgat gcgcctcttc
      541 cggggcgccg ttccggagat ggagaaggcg ggagggggtg ctttcgtcac catagcatcc
      601 gtgggcggca tacgcggagc aagggccggg gccgcataca cggcgtcgaa gcacggggtc
      661 gtcgggcttg cgatgaatgt cgcctatgcg tatgcgaaga agggcatccg gtcgaacgtc
      721 atcgctccgg gcggcgtcag caccgatata ctcaccggca agagcctgag ccgggagggg
      781 acggaggtct atatggcggg tatggcaacc aacccgcgta tgggagagcc gatagaggtc
      841 gcccgggtgg cgctcttcct ggcctcggat gaggccagcc tggtgaacgg ggcggtcatc
      901 accgccgacg ccgggtggac cgcctactga tcgcggccgt tccggccact atcgagcata
      961 aggcagttat cccggcgggg agactcttac agtatgtctc cccgttaccc ggcacccgcc
     1021 ttcgacgtct tcaacgtcta cttcgagcgt atctacgatc ccaccatgca cgccgtcttc
     1081 acgtttgacg gcgggatcga tgccggggcc ctgcggaagg cgacgatgag gacggttgcg
     1141 tccgacccct acaccaggtc ccggtacgcg gaggttgacg gccggcccgt ctgggaagag
     1201 atcgcggagg aggagtgggc gagggctttc gtcgttctcc cggctggtga agatacaggc
     1261 atgccaccgc cgccgctcga cgtccgcgcc gggccgcagg tgcgggtcgg tctctatccc
     1321 cgggcgggag gggatatcgt tgccgtcacc tgccaccacg gtttctgcga tgtctccggc
     1381 accatggccc ttgcccgcga catctttgct acctaccggg gcatcgtggc cgacccgggc
     1441 taccggcccc ctccccgcga gccctacgac cggagcacgg atcggatact cgcgctctcc
     1501 tccgacgagg agcaaaaacg ggcgctcgca gaggaggaac cgttcatcga ccggtggcgt
     1561 ttccccgccg ggcggatggg acggggaacg ccgcggatcg cctcacggac gcttgcgccg
     1621 gatcgcctcg agcgcatgaa ggcattcggc agggaacacg gggccacggt gaacgacgtc
     1681 ctgatcggtg cgttcttcct cgccctgctg gatatccgga atgaccctgc ggatcggggg
     1741 gcgccccggt cgatcctgac gtcggccgac ctccggagga gatacccggg atgccacggc
     1801 gaggggctgc ccgtgaacct ctcggtcgcc tatgatgtca ccctcaccgt ggaggagggc
     1861 gcggggctgg aagatatcat cggccgggtg acggcgataa cggcccataa gaaggcaggg
     1921 aaccccggac ttgccgccat cctcttctac gagggtatca tggcgggcgg gatgccggcg
     1981 gtgcgggcgt tcttcgatga gatggccggg cgatacacct cgtcgggcaa caagaacccg
     2041 gtcttctcga acctcggtat atttgacccc ggcgactacc tccccatccc gggagaagac
     2101 ggcagagtgc tcgaccttct cgatatccag tacctcccct gcgtttgctg gccgtacggg
     2161 ttcctgatgg cggcctcgac cttttgcggc cgcctgacgg tcacgaccgc ctacgaggaa
     2221 gggccatact cgacggcaac cgtggagcgg ttcctcggtt gcgtggacgg atacctcccg
     2281 tgacctctcc cgcgggagag actcgcgccc tttttctcct gccgcgccca ccgttcctgt
     2341 atggacgcac ggagattcag cgtaacaccg gtcggcaccg tgcatgccgg cagaggggtc
     2401 ttctcgatcg agattgccga accgttccgg cccgctctta cggaactctc ggggttcggt
     2461 cacatgctcg tcctctggtg gtgcgacggg gtcgatacgg tagagtgccg gagcgagacg
     2521 acctgcgaaa agccgtacac gagaggcccg gatacgatcg gcatattcgc cacccggtcg
     2581 ccggtccggc ctaacccgat cgcgctctcg gttgccccgg tgctcggcgt cgatgccgcc
     2641 gcgggcatca tcagggttgc gtatatcgat gccgacgacg gcacgccggt cctcgacatc
     2701 aagccctacc tcccgtgcaa cgagcgggtc agggacgcga aggtgccggc atgggcctct
     2761 cgctggccgc agtggtacga ggagaacgaa gggtttgact gggcggcgga gttcacgttc
     2821 ggggattgaa ctcctgccgc ccttttgatg ggagtggccc catacggggg gttacttcac
     2881 gcgatcgagc gggatgccga gcgcatccgc gacgcccttc ccgtaggccg ggtcggcctt
     2941 caggcagttt gcgatgtgcc ggaccttgat gagttcgggc gcatctccca tggcgcgcgc
     3001 ggtgttctcg aagaggacct gctgctggcc cgggctcatc aggcggaaga gtttgcccgg
     3061 ctgggtgtaa tagtcggcat cgtcctcgcg gaagttccag gaccctcccg cgccggagag
     3121 agcgaggaac ggttctctat attcgggctg ttcctgccat tcgccgaaac tgttgggctc
     3181 gtagccgatg gtggtgccgg cgttgccgtt cacccgcatc tgaccgtcgc ggtgagaggg
     3241 gttcgcgaca cacttcgcgg cgttgaccgg gatctggtgg tggttgacgc ccaggcggta
     3301 gcgctgtgca tctccgtagg caaagaggcg cccctgcagc atcttatcgg gcgagaaccc
     3361 gatgcccggc accacggcgg ccgggttgaa agccgcctgc tccacgtcct ggaagtagtt
     3421 ctcggggttc cggttcagct cgagcacgcc cacctcgatc agggggaact cacccttgta
     3481 ccagactttg gtgaggtcga aggggttgta gggcatggcc tttgcctgtt cttcggtcat
     3541 cacctggatg tacatcgtcc agcgggggaa gtcgcccttc cggatgctct cgaagaggtc
     3601 gcgctgatgg ctctcgcggt cttttgcgac gatcgcctcg gcttcctggt cggtcaggtt
     3661 cttgatgccc tgccgggtct gcaggtggaa tttgacccag acgcgttcgt tttccgcgtt
     3721 gatcatgctg aacgtgtggc tcccgaaacc atgcatgtgg cgataggaga gaggaatccc
     3781 gcgatcgctc atggtgatgg tcacctggtg cagggattcg ggaagggatg tccagaagtc
     3841 ccagttgttg gtcgcggagc gcatgttggt gtgggggtcg cgcttgacgg cacggttgag
     3901 gtcggggaac ttgtgggggt cacgtatgaa gaagacgggg gtgttgttcc cgaccagatc
     3961 ccagtttccc tcctcggtgt agaacttcac ggcaaagccg cggatgtcgc gctcggcatc
     4021 ggccgcaccg cgttcgccgg caaccgtcga gaagcgcatg aagacgtcgg tcttcttgcc
     4081 gatatcggag aagagcctgg ccctggtgaa ccgggtgatg tcgtgggtga cggtgaacgt
     4141 accgaaggcg ccggacccct tggcgtgcat gcgccgttcc gggatcacct cccggttgaa
     4201 gtgggcgagt ttctcctggt accagacatc ctgcatcagc atggggccgc gtggaccggc
     4261 ggtcacggca ttctggttgt ccggcacggg tgcgccggca gcggtggtca gtctcttttt
     4321 gtttgtcata aatcactcct cgtttctgtc cgctggatga aacgggtacg ataatccctt
     4381 gatggagact ctatttgtgc gtatttatac cttgtccgga ggtatccggt gaaacggcag
     4441 gcggtcccga ctccaacggg ggtggaggag atactgtcta cggggaaggc ggccggggcg
     4501 caaagggcgg cgcggcacct cccgcccgac cgcgaggtct atcacccccg ggtgcccaac
     4561 cccggtccgt gacggggatc atcgctttca gcgatcttgc gtgggggacg agggaacggg
     4621 gtttgcctgg cggcggccgg gtcggcatcg cctcgttcat gcgcatcgtc gatgaagcgg
     4681 atccggcaat cgtcatcttt gccggcgatg ccgcctacga ccggtgttcg cgctcggggc
     4741 tcgacgagac ggaggtcttc gtcgaccttc tccgggatat tgcagcggcg gggaggcatt
     4801 gcatcgtcgt cgaggggaac aacgacaatg cgcgcggcac ctacggccgc gtccgggagg
     4861 cggcggaggc aatccccttc ctccacgaag tgtcggggaa ggtggaggcc gtccacggca
     4921 tccgtttcct cggcgtcccc acaggaaagg agaggaggat ggcccggtcg gccgaagggc
     4981 cggtcgatat cgtggtggcc cacgcaccgc tcgccgaccg ggtctggctc ttcgatctcc
     5041 ccgcggcgtg catcgtcacc ggccactacg gcatgatggt tgccgggatt gccgggaagg
     5101 cctatgtcgc cctcgactgc tccccggcat cctatgcggc gatcgagtgg gaggggggct
     5161 ggcggcggat cgcgtacgtc gccggggcgt gccggatcag tctcagcccg ggggaggggg
     5221 tcgccgcgac cgggtgcgat gcagaagaac gccggcgcct gaccgggggg ccgggggaac
     5281 tcccgtaccg ggccgagatc gaggcgctgc ggcaggcaaa ggacgaagtc gcggtgctcg
     5341 ggcgggagga ggtcgccggg cggctgctcg cgatggggat aaaaaagacg cacatcgagc
     5401 gctacctcgg gaggcagggg cgccctgagc cgtgatcccc ggctgcggcc gggatgacct
     5461 ctcccggcag cacctcgttt tcagccggcc ggagggcgca cggcagtaga gaagggaata
     5521 agttttatcg aggtaatgga tgaataactc ttcaatggat gacctggtca gggagcggat
     5581 actgcaaaag gttgaggtgc tcatcgatcg gatcacgttc atcgagcacc acctctcgga
     5641 tgccgtgatg aacgaccgta tcctgcgcaa agcgctctac aaagagtttc aggaggccgt
     5701 agtatcagct acggatatct gtgccctcat ccggcggata ctcctctctt cggcacagga
     5761 tgattacacc aatctcgact atctggtggg cgaagggatc atcagccggg agacgggaag
     5821 agatctcaaa gctgcaaacg ggcttagaaa ccgcctggtt cacgaatatg acggcatcaa
     5881 cgaccatatc gcgtatggcg caatgcagcg gctgatgccg tctctccgca ggttttcagg
     5941 cgaggtgctc acatggttga atgcgaacag gacgagatcc tgaaccactt ccgggacctc
     6001 ctcccttcga tccgggctat tctgctcttc ggttcgcgag tcgccggcta cgcgggccca
     6061 acgtccgata tcgatatctg ccttatctta accggtgagg cggacaggac tgcggtgcat
     6121 gacgggatgc tcatcgcccc ggagcactac gatatcgtcg tctacgacga gatcccctgg
     6181 tacctcaggg gagggatact cgagcggcat cgtatcatct acgcagaaga ccccgacgac
     6241 ctcgacttct ggctctacaa gcagcgtcgg atctgggggg acatgaagcg gcgccagcag
     6301 agggcatcct ccgaagatct cctgaagcgc atagggtatc gctgatcggc tcaccccggg
     6361 atccaggtcc cgcactacgc cgcccgcgcc ttcccccggg cgtccggttc gcgcctgcgt
     6421 gagcgaggag gcccgcggcc gggaacatat tcgccgggag agattgcccc gttacagccc
     6481 tatctctacc ggatcgttta tccgccgcct ctccgtaacc tctctatgga cccggtcatc
     6541 gaggcgatac ggcaggagtt tgcggcgcac gcggaccccg atcgcaggaa gagcagtctg
     6601 cggttcttca aggaggagat ccggtgctac gggatgaaga ccgccaccgc agttacggtc
     6661 gcaaagaagt actggaagaa ggtcaggtct cgcgagaagc aggagatctt cggactctgc
     6721 gaggagctct accggtcagg gatgatggag gaggcgttcg tggtctccca gtgggcccag
     6781 atgctcggcg aacgctttga acccggcgat atcgccgtct tccggcactg gatcgacact
     6841 tacatcacga actgggcggc atgcgacggc ctctgcaacc atgccgtcgg ggacttcgtc
     6901 gtccggtacc cggagcatat cgaggagctg aagcgctggg cgcagtcgga taaccgctgg
     6961 atgcgccggg cggcggcggt ctcgctgatc gtgccggcaa agcacgggga gttccttgac
     7021 gaagccatcg ccatagccga tcttctcctt gccgacacgg acgacctggt gcagaaaggc
     7081 tacggctggc tcctcaagga ggcgagccgc gaacacacgg acgaggtctt ctcctacgtc
     7141 atgagaaaca agcgggtcat gccccggacg gcgctccgct acgccatcga gctgatgcca
     7201 aaagacctga aggccgaggc gatgaagaaa gattggtgag aggtccctta gaaccggacc
     7261 cggggagcgt ccgcctcccg catgttgtgc tccatcgtcc agaaatacgc ctcgccgtgg
     7321 gccacgcgga agacggcgag tttcggatcg gcggggccgg agatgccgat ctggaggagc
     7381 cacggccgat ccccgacgag gcgctccttc aaggccgcgt cgtcgaggaa ctcgaccgcg
     7441 cccgcgaccc gcatcatcgt gttcacgtcg ccgggggcat agaagcagag ctcgaccttc
     7501 gggttcttcg caagttgccg ccagacggcc ttcggggtcc cggtgtggta gtagaacccg
     7561 gtcgcatcgg caaaccacat gacaaacgcc cggacccggg gctggtcgcc gtccatcgtc
     7621 gcgatgtagg tcacagggtt ttcgtttgcg aatttcacgc actcggaaaa gtccattctt
     7681 cttcctcatg cattcttacg gtgagttgca tgaaaagggt tgcggagacc gtcatgcggg
     7741 acccggcgcc tgccggtaga acttcgcccg ggcgggcagc acagagcaca ccccccaccg
     7801 ggtgcccgcc cgagttttat cctcacgcac gcgggggagc gcagnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn nnnnnnnnnn nnnnctcacg cacgcggggg agcgcagtct tccccactcc
     7981 ccccgatagt ccctccgttt tatccctgcg cacgcggggg agcagctgcg gcaccgggga
     8041 agaacctccg ggagcagccc ggaggatact ctcgcgggtg ccgcagggcg caggcagccc
     8101 ggtgaatacc ggaatggggg agctctatct gccgggatga ccgtatgagt aggttttaac
     8161 actctcaagg agaatctcga tacgatgaag agcccttttc acgtcatgat catccccacg
     8221 ctcggttgcc cctcgaaatg ccactactgc tggagctcgg atgaacgttc tccaagaatg
     8281 agcatcgaga cggtccggga gataacggag tggctcaaag gctaccagtc caaccagatc
     8341 accatcacct tccacggggg agaaccgctc cttgccggcg cggagttcta ccggcaggcg
     8401 ctccccatac tctcggaggg gctcgcccac cttaccccga cgtttgccct ccagagcaac
     8461 ctctggctgc tcacccccga tatggcccgg atacttgcgg agtacaggat ccccatcggt
     8521 tcgagcatcg acggccccga ggagatcaac gacgtgcaga gggggaaggg atacttcaag
     8581 aagaccatgc ggggctacga tatcgcgcgg gcaaacgggc ttgaggtcag gttcatctgc
     8641 acgttcacca accggtcggt cgagcgcaag gaagagatct tcgacttctt cctgcagaac
     8701 gggttccccc tcaaactgca cccggcgctg ccgtccctcc ggagcgagaa cccaaaagag
     8761 tgggcgctcg aacctgaggc ttacggagag ctgctggtat acctcctcga caggtacctg
     8821 gagaacctgg gccgtatcga ggtcatgaac atcaacgaca tctgcagggg cgtctttacc
     8881 cggcacggca ccgtctgcac gttcgtggac tgcatgggca ccacccttgc catcgggccg
     8941 gacgggagca tatacccatg ctaccgtttc gtcgggatgc cggaatacgt gatgggcaac
     9001 gtctacgacc gcccgggccc ggaagacctc gcccggtcgg atgcctggag gctcatgcag
     9061 gagttcaggg agtacgtcga ccgcgaatgc aaaggatgtc cccatataaa gtactgccgg
     9121 ggtgggtgcc cctacaacgc gatagcgccg acagacggcg agatccgcgg cgtcgacccc
     9181 cactgcaccg cgtaccggcg aatattcgac gagatcaccg accgggtgaa taaagaactg
     9241 tacgactcca ccgacatgga gatgggcgac ttcggttcga ggcagcggag gagggccaga
     9301 cccggtatca tgtcgatcct gcaggcgatg gcggagagat aagcggtcgg acccgagagg
     9361 ctcacgcctt ccggagggtg aaccggaacg ccgcaccctc cccggggcgc cccgccaccc
     9421 ggtcctcgat ccagatcctg ccgccgtaac gggcggtgag catccggcag atcgagagac
     9481 cgaggccctg actccccctt ttgtcgttct cccgctcgaa ccggtataag atcgtctcct
     9541 tcatgtcatc cggtataccg gggccggtgt cggcgacggt gacgacgacc tcttcgccca
     9601 ggtcctcgac cgccacggcg acattgacct ttgatccgcc gtgctttgtg gtgttgccga
     9661 tcaggttcgt gaagacctgg gggagaagat cgtcggcgaa gaccttcagg ggcagcccgt
     9721 cgtagcagat agggagatcg gggaagtgct cgatctcgct tttgatgacg gcatcgagat
     9781 cgacaggttc aagcccggcc cgggtctcgt ggatctttct gatggtggcg acgtttgccg
     9841 tgatctcgat gcttttgcgg ataccgtcct tcagtttctg agcatgcccg accacttccc
     9901 cctcgagttc gtcgatgagg aggtcggcgt atatgttcgc gacgttatcg gcgttcttga
     9961 tgtcgtgggt gaggatatcg aggtagagat ttgcctcccg gttcgccgcc tcgagtctct
    10021 ggtggagcat tccccggagc atcccggccc cgatctcccg gccgaccgcc tcgaggagtg
    10081 cacgctcttc gggagggaag gactgtcggt tcctgctccc gacaagaagc gccccggcaa
    10141 cgtcgggctc gacggtgaac ggggtgcagg ccagtgcggt aaacccgata tcgcggagga
    10201 gacgggatac cggactatcg tcctgctcga tgtaacgggg agcgccggtg gcgcatactt
    10261 cggcgtacga tgccctgaag agttccccgg cccgttccag acatctcttc ggcatacccc
    10321 gttcgtaacg aagtgcgacc ccgctccttc cgggttcggt gaggtagatg ccgccgccat
    10381 cgtagccgag gaggtcgagc gtctggtcaa gcgccgtctc caggagttca tcgggggttt
    10441 gagcctgggc ggatatgccg atgatcctgt tgagcagcag gagatggcga ttgcttgcct
    10501 gcaaccgctc ttccgcctgc tttcggtggg tgatatcccg catgacgacc tggactgcag
    10561 gcctccccgc aaagaaagcc cgggtgcccc gcccctcgac gaagaccatc gtcccgtcaa
    10621 gcctgacgac cgggagctcg acgagcggcg tctcccccct ctgcaggttc cgggcagcga
    10681 aggatctgac ggtctcgcgg gagccgggat ggatgatatc ggcgacgttc gtcccgatga
    10741 cctcgccggg gtcttgcgcg ccaagaagcc tcacgccggc ggggttgatg taggtgacga
    10801 tgccgtcctg gtagatgagg atcgcttcgg ccgagagctc gacgagcgac cgatacttct
    10861 ctttgctctc ctcaagctgc agctccatct gctttaaggg ggtgatatcc aggaccccgg
    10921 caaggaaccc gcggcaagcg cctgaggcgt cgataaccgg tgtggtcgcc atcagggcat
    10981 ggatgcggat accgtctcta cgaatcagat cgatctcgaa gatctcgtgc ggaaacgtat
    11041 ccaggttctg caggtagtcc tccatgcacc gggcaccttc ttcatcggtg aaacgggagg
    11101 ccggcgctcc gacagtctca gcgatggggc aaccgatgat atcggccagc ctcttgttcg
    11161 caaagaccac gatgccctcc ctgtcgatca gcccgatccc ctcattcagg ttctcgacga
    11221 ggaaccggta ccagtcgtcg tcctgccgca ggagatcgcc tatccatccc ggggaggcgg
    11281 aatcctcccg gggcacgggc tcgaaacggg caactcgcat accctggaac agcccgctcc
    11341 cgacggtttt gcacgagcag cagacccggt tgctcttgcc gccggacgtc aagagcgtgc
    11401 acgagaggtc gagagatcgc tggcggccgg agaagaactc aaggaggtct ctcctgcacg
    11461 actcttcggc gattcgaggc gccagggtct ggtggacgaa tcgggtcgcg tcggttccgc
    11521 cggccgcatc tcggtcgata cccaggatgt gccggagacg cttgttgatg caggcgatcc
    11581 gattatccga accgacgacg agcagaccct catcgagctc gtccaggacg gcggagagcg
    11641 cgctgatact accgtatgca ggtatgggtt cgtgcattgg ccgggatcac ctcttccctc
    11701 aggatgtttt tatattatgt taagataaaa tgttcccgtg cgattcagat gaacccgggc
    11761 tcggttcaga tgaatcgagg agttcggaac tccttcgggc ggatctgtct gactacgaga
    11821 tgtatgctgt tgcttttgta catataaacc tgtttattct ctccggcgga tcttcacgca
    11881 tcacctggct gagggggcac gcacccggcg ttcctgccat tgcgcgcaca ggggtgctcg
    11941 gggcaggccc tccgcacctg aaacggccgg catatttacg ttctttgggg ggcattccgc
    12001 cgcatggacg gattcatgca cccgcaccgt gacggtcatc cgatggttga ggggggcccg
    12061 cgtgcctgtt ctccggaggg ggtatgacgc tcatccggct gctcgtcttc ctcgccgtcc
    12121 cggtctaccg ggacgtaaat cgcccggggg tgaacggcct gctccggttc atccccgcct
    12181 tcctgatcct ggtcatccgg agacccgggg ccggcgatgc agcgatggat ctcctccggc
    12241 agcgctgcgc gcgcggcgag atcgacgaag aggagttcca gcgggagcgg aggggctgac
    12301 gtggggcacg gcgaggaggc gggccgggag actcaccacc acatcttcga cgactaccga
    12361 cggcgattta tcgtatcggt catcctgacg gtcccggtcc ttctcctctc gttgccggtc
    12421 ggggcgctcc ccatcgcctt cccgggctcg gcgtacctcc tcctcgccct cgcgaccgcc
    12481 gtctacctct acggcggctg gccgttcctc tccgggttcg tccacgaact ccgggcaagg
    12541 atgcccggca tgatgacgct cgtcgccgtc gcgatcaccg ttgcctatgt ctacagctcg
    12601 gcggtcgtca tcggtgtcgt cggtggagag gggttctact gggaacttgc caccctgatc
    12661 gacgtcatgc tcctcgggca ctgggtggag atgcgctcgg tcctcggggc gtcgcgggca
    12721 cttgaggagc tcgtcagggt catgccctcg gaggcgcacc gggtccgcga cggggggacg
    12781 gacgacgtgc ctgtcgaccg gctcgtcccg ggcgaccggg tgctcgtccg gcccggtgag
    12841 aaggtgccgg tggacggcgt gatcgtggag ggggggacga gcgtcaacga ggcgatgctc
    12901 accggggagt caaggccaat cgagaaacgg ccgggagaca cggtcatcgg cggcgctatc
    12961 aacggcgagg gttcgatcgt cgtcgaggtc gagaggacgg gcgcggagac ttacctcgcc
    13021 cgggtgatcg acctcgtccg gaaggcgcag gagagccggt cgcggaccca ggacctcgcc
    13081 gatcgggcgg cgttcttcct ggtggcgatc gccctttcgg tcggcgcggt gacgtttgcg
    13141 gtctggttcg ccatcgaggg gaccgggttt gctgtggagc gggccgcgac cgtgatggtc
    13201 atcgcctgcc cccacgcgct cggccttgcc gtcccgctcg ttgtcgcggt ctcgacggct
    13261 cttgcggccc actcggggct cctcatccgc gaccgagcgg cgtttgagcg ggcgaaggac
    13321 ctcgaggctg tcgtcttcga caagaccggg accctcacga cggggacgtt cggtgtcacg
    13381 gatgtacttg ccttcggcgg tgccgaggcg gatgtcgtca gggcggcggc gtcggtggag
    13441 gcgcactccg agcacccgct cgcccgggcc gtcgtccgga gcgcggagga gcgcgggctc
    13501 tctcttctgc cggtcgaagg gttccgggcg ctgccgggga agggcgtcga gggttcggtc
    13561 ggcggccggc gggtctcggt ggtcggcccg ggctacctcg cggagcgggg gatggcggcc
    13621 ggggacgagc gggtcgagcc tctccagcgg cagggaaaga ccgtcgtctt cctcatcgag
    13681 ggtgatcggc tcaccggggc gctcgctctc gcggatacca tccggccgga gtcgggggag
    13741 gcaatctccc ggctcaaggc gatgggcatc cggtgcatga tgctgaccgg cgaccaccac
    13801 gacgtcgccg cgtgggtggc gggggagctc agcctcgacg aattctttgc cggggttctg
    13861 ccgcacgaga aggctgcgaa gatccgggag attcggcgcc gttaccgggt ggcgatggtg
    13921 ggcgacggga tcaacgacgc cccggcgctc gtcgaggcgg acctcggtat cgccatcggg
    13981 gcggggaccg atgtggccgt ggagagcgcc gatatcgttc tggtcaggag cgacccccgt
    14041 gatgcggcgg cgatcatcga tctcgcgaag aagacctacc ggaagatggt cgagaacctc
    14101 ctctgggcaa ccggctacaa cgcggtcgct atcccgcttg ccgccggcgt gctcttcagt
    14161 gccggcatcg tcctcacccc ggcggccggg gcggtgctga tgagcgcgag cacggtgatt
    14221 gtcgcggtta atgcccggat gctccgggtg tgaggggggg tcatctacgt gggtttgagg
    14281 tgctcttttt gcttgagcag ccatctccgt gcacgtaggt actctgattc cagatcacgg
    14341 cgcgttgttg tgctggatat ctctcctatg atctcccgga ctgcttcaag gtgtttctct
    14401 gcctcctctc cgctctgcga tgtatggata gcgagggttg cattcctgat atgccatcgt
    14461 gccaggaggg caccggcaca tacgtcatcc tcgagaaact cctcaacctc agactgaagc
    14521 cagctgtcac gggagatggt actatcgcga atggatttat cgaggtgccg gcggacctgt
    14581 tcgaaataat tctcactggt ttcaaacaga cccataccca ggtatgcctc accaaggtga
    14641 aatgctactt tgatcggagc gatactgagc gattctgcag tttcaagata cgcccttgcc
    14701 tcgttgtacg aacccatgat taaagaggac tttccaagcc agtagtacat cttcgcgata
    14761 tccttactct cgcccttacg gagctccaga gcgatcttaa agttattgga tgcttcctcc
    14821 gatagttccc gcttttcttc agggttatgg gagcgctttg cacgcaatag atctacctgc
    14881 cctatacgga agtatatttc cggttgctcc ggatcacact ggagggcgag gcggtattca
    14941 ttgatgcaat ggtcgaggtc attaatgccg tagagtccct gccccctgcg cagatgagaa
    15001 tgagaacatc ccggatcaag cagtaaagca tcatgcgtgg ttttaatcga cgtcgggtag
    15061 tcttttttct ggagctgggc atgtgcttga gaggcgtaga tagcatttct ccgtatcctc
    15121 tccgggtata acgtctttaa tgattgaagg gactcgataa acatctcatc ggctttaccg
    15181 ggctctccat gacttagaaa gttgataccc tgtacccgga ggctctctcc cttctgccag
    15241 tcgtcgtaga tcgatctaag gtgttcaatc ccgctggatg ggatctctgg gggagacagg
    15301 tcgatggcct tggaactctc acatttcctg gtttcttcca agaatgccag tgatcttcga
    15361 attaataagg taatgtcctt gcattctatc ctattctcaa attcttcggc gatgcgatag
    15421 agatgaagag gctttaactc ttcgatatag tttaagaact gtatgagtgc atgatctcgc
    15481 ccctgtcgat agatcccggg catagcactt cttgaatccg gatatccgac ctgatgtttc
    15541 gcacccggtt tctggatcct cttaatgttg gctatcgact cgttgtatgc cagggcaaga
    15601 tacatccagg cggttggaag tgtgtagtcg atcgatatgg ctcgtttaaa tgattctatt
    15661 gccagatccg ggcggttggc cataaggtgc agtcctccta tccctagatg cgggttcgcc
    15721 tttgagggat tgagcgctaa tgcctgccgg tatattcgca tctggatctg cgataataac
    15781 cagagcgaca ggcgatctat cgttgagtga ctcttctcgg atccgctatt gccggatttt
    15841 cgcttcaggg atttcagatg gagggtatat gccatgttgt tcaggcattt gtatgcccga
    15901 tctctatctt ccgtcactgt tacaagtgga ttggctctat cattcttttg tgatggtcgc
    15961 aggaggttct gacagagtaa cttccatgac tgctctactg ctatacgacg gcactgtatt
    16021 gcatgatcaa ggctatcgtg gccgccactc tcgaacgggg gaagctttcc aggatagtcc
    16081 agatctcctg aaggatcagg gcggtttata acatccggac gtttttcagt gatatatcct
    16141 ctgagcccgt acgcaaaccc ttttaattcg taggctttag gacaaactgg ttggagcatt
    16201 aaacatgcat cacacagttc aattacccgg cacaagctcc agtaatcgcg aaaaattcca
    16261 tcatcacgct ccagggaacc ccttttttcg atgtccccct catagagatg gtcgtatgcc
    16321 tcgtcaaaat aattcagcgc caacgtgtaa tatgattctg cctcttttgg gcgtgaatgg
    16381 gtactcaaaa taaataccct ctctgctgct tgccatttct tctgctcgcg atagataacc
    16441 ccgaggttat gatacgcgtt atggtaaccg ctatcttcgg agagggtttc gataaacttt
    16501 tcttcggctc gaatcaaccc gatagttcga ccctgtggct ttgccttact atggagatat
    16561 tcctcaaggc cccggttaaa ggcctggacc gctttccact cagtggcatc atcggtatat
    16621 atcgatgtaa atatcctgca cgcaagttca tggatcatat cgttaagagc cggctctgac
    16681 gatgtgccat caccatgggc tttctttggt cctacaaccg tccagctgag tggttcttca
    16741 ttgtcgatcc tatgggcaac caggtggagc ctatcgtctt cttcaaaaag gattccagtg
    16801 atacgggtac ctcttacaac ccgtgatgca atatctctga ttatttcttg aggaaggtta
    16861 atcgggccta tgctcagcgg ttgattgctc tgggagatat ttttggaggt agttgagtaa
    16921 tccgcagtga tacgtggagg ttccggccga tagccgtatg ttacatccat tgatccggct
    16981 acgttctcgt acaatgagcg tatccgggtc agctcggcag ccaatcgatg gtgaagatct
    17041 tcagcgacat taggcacatt ctcctctaaa ccccgtacat gattcttttg acgacaatcc
    17101 ttgaactcat cgatagtcac gattttggag gcaacattcg cacgtcgggc gagcgagaag
    17161 gccagaaaaa gcagtaaaat aactccgaat aactcaactc cttttatgag agaaatgctc
    17221 tggaaccata tgagcaatac attcatgata gcgggtagga catctatcga aacccattcc
    17281 aggtttatcg ggctaacatc catctgctta tccgcgaggt tggcgctcaa gcagtcaaag
    17341 gctaagacag caattgcgat gaggacaaac tgaaggcgaa gagagtagta taatccacgt
    17401 ataaccgtgt aagattgtcc aacctgatga ctgaagcaat acattaaatc tcgatagtaa
    17461 gaacccgccc atacggaaag tagccaccag agggaattta cttcctcagg gtttgtttgc
    17521 ttttcaacca ttccgcattc ttgcacccaa tgaagttatc tcaaaaaatg ctcaccccat
    17581 ctacctgaaa agatcatccg ccagtaactc cgagaggcgc cctcctcctc catcatagat
    17641 atactttacg ggaatccaga tggaccgggt cgaggagggg ataagcccta gacagcggag
    17701 aatttcgaat aacctcccta ttccagggcc catcaaatca gagagcatcc taatttgaca
    17761 gcatgttttt cagcgtgatt ccccctgtta ctccatcagg ttgctcttgt caacggcggc
    17821 gtaaaagtgt acagattcag gcggaataaa agtgtacacc tgatcccata atggtatcga
    17881 aactcatccc ggcagggtgt gggagtagat cccctgtctt cgtcgttctt tcagccggta
    17941 gctatcgcct ttaatgttca atgtcgtgga gtggtggaga attcggtcaa gaactgcagc
    18001 gg
//
LOCUS       DAOSEE010000028        17579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1308_length_17579_cov_215.340733, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000028 DAOSEE010000000
VERSION     DAOSEE010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 17579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17579
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1308_length_17579_cov_215.340733"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(298..747)
                     /locus_tag="PLI76_03590"
     CDS             complement(298..747)
                     /locus_tag="PLI76_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOI61145.1"
                     /translation="MLVARFLVCDAFTRGYDLPVLSRILDESERTGEVPLKGAIGALF
                     CLAVFGREYAAAGLVTVGMLDSISTLVGLRFGRHTLRDSKPLEGTLTGVAAVALALAF
                     LIPWWAATVVAAAAGIVEAFFPLEDNVAVQVGACVTLAVVRIVMPGW"
     gene            979..1746
                     /locus_tag="PLI76_03595"
     CDS             979..1746
                     /locus_tag="PLI76_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04255 family protein"
                     /protein_id="HOI61146.1"
                     /translation="MVEVRKYVNPPAAEVICEFRFPEEIPWDLTYPGILYGHLKDTYP
                     KRDQRYVREVVMLLGPEGLREELLVAERSIFLAEDEGCAVQVGPRLLSVSCQKPYVHW
                     EAFSERIHGAFDRFRDVISADAVRTINLRYVNLIEIPEREVTLSDYFAFYPTLPPELP
                     RTPAGFITGCEFAFHDNRDNCRVELTDAVPESTTNNAFLLNIDYFLTEEGSITLDGVA
                     DWLEIAHTHVRDIFEACIKDPLRDLFTIREEVPAAAR"
     gene            1753..2556
                     /locus_tag="PLI76_03600"
     CDS             1753..2556
                     /locus_tag="PLI76_03600"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOI61147.1"
                     /translation="MGDIVRKKWMTMMDAHAGAVDQVYGGPVGLIWEMLAGEGADGDT
                     GALAERAGLGRESRVFSLLSGPGEAARRIARKYGCTVIGLVPVPQVVDEAVRKTEEAG
                     LAGQVDFQQGNALDMSFHDGVFDTVWGEDAWCYVIDKDRMIREAFRVLKPGGTLAFTD
                     WVEIGPMSDDEWRTPETFRIFPYFETVERYAELLERNGLRLVDGGEVPGDPIRQVGNI
                     RKRLRDSRDAITELIGEEAYDDARRGLDLWERAAGRGLIGRGWLLAEKP"
     gene            complement(2568..2777)
                     /locus_tag="PLI76_03605"
     CDS             complement(2568..2777)
                     /locus_tag="PLI76_03605"
                     /inference="COORDINATES: protein motif:HMM:NF022829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HOI61148.1"
                     /translation="MIGTALPPGVTVSRVHEYLRDVPYPVTREELIDYARSAHAPDEI
                     MRALDLLPEKRYANPAIVTETLGMI"
     gene            2906..4405
                     /gene="malQ"
                     /locus_tag="PLI76_03610"
     CDS             2906..4405
                     /gene="malQ"
                     /locus_tag="PLI76_03610"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOI61149.1"
                     /translation="MIQVRGSGILLHITSLPSEYGIGDFGPAAYRLVDALAQARQHYW
                     QILPLNPTQTEYASSPYSSPSAFGMNTLLISPDILVRDGFLRRADLDPAPEFPERRTV
                     YEAVAWYKDRIFSVAYERFLHSGPERGYEVFYDRNAWWLDDHALFVALKAHFRGQEWN
                     RWPEEIRGRQPEALEDMRRRLADKIRKETFLQYLAARQWSALRRYCAGRGIQVIGDIP
                     IYVAYDSVDVWQNPGIFKLDDNLRPTVVAGVPPDMFSKTGQLWGNPIYDWALLRDRGY
                     DWWVNRIARAGELYDLFRIDHFRAFANYYEVPAGDTTAENGTWVDGPGTDFFEVLARR
                     FPCFAIVAEDLGANTPAVQELLDRFGFPGMKILLFAFGAELPGSAHAPHNYVPNLICY
                     TGTHDNNTARGWFEEEASEEDKQRFFAYIGREVAADEVHRELVRLAMTSVARASITPM
                     QDLLGLGAAARMNYPSTTSGNWEWRMTPVEFAGAPLEWLRNLTVLSGRG"
     gene            complement(4407..4604)
                     /locus_tag="PLI76_03615"
     CDS             complement(4407..4604)
                     /locus_tag="PLI76_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61150.1"
                     /translation="MSKVVRVDEEALEVALKYGKNLSAGIMMMEELLRKQEKVRRDYT
                     AIEEMIRRTIREELEVLTSRY"
     gene            complement(4760..6532)
                     /gene="glgP"
                     /locus_tag="PLI76_03620"
     CDS             complement(4760..6532)
                     /gene="glgP"
                     /locus_tag="PLI76_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOI61151.1"
                     /translation="MAVRDPICGMPVDPAKAAYSVEVRGVTYYFSSELCRDTFVDGEK
                     IAYFSMEIGLESDIPTYSGGLGVLAGDVIRSSADLLIPMVAVTLVNRKGYVRQRLTPE
                     GDQIDLPDEWDPKKHMRELSRRVTVRIGDANVTIRAWLYDYWSPVGGLVPVLFLDTDL
                     PENAPEDRGITDHLYGGDQECRLKKAIVLGIGGARMLTALDIKVGKYHINESHSSLLT
                     LELLRRPGMDEEKVQTHCIFTTHTPVAVAFEKFPRDMVVHLLEGEIPPELLDRYIARG
                     GLALATLAFRLSRYINGVTTAHMNFSRQLFPGFHIRAVTNGVHPHTWTSVPFRNLYDR
                     YISGWAQEPELLARVDTIPREEIRHAHQQAKQALVNYVKEKNNVDLDPRALTIGFARR
                     AAAYKRATLLFSDLDRLREASRKGAIQLVFSGKAHPTDVAGKKEIRAIHDAMHDLEGE
                     VTGVYLENYTMDIAGLMTAGVDVWLNTPLPPYEASGTSGMKAALNGVVNFSILDGWWI
                     EGWIEGTTGWAIGPGPGAKVSEAERLREALQDLYSKLEYLIVPTYYRWEDEWATIMRS
                     SIAKIAYFFNTHRMMRRYATEAYL"
     gene            7025..9475
                     /locus_tag="PLI76_03625"
     CDS             7025..9475
                     /locus_tag="PLI76_03625"
                     /EC_number="7.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasma-membrane proton-efflux P-type ATPase"
                     /protein_id="HOI61152.1"
                     /translation="MVESQTISTDKARSMAVDDLYQALSSGQGGLGQSEAESRIRKFG
                     PNEIVEKEESEILKFFRYFWGPIPWMIEAAVIISAIIRHWEAFAVILALLLINAIVGF
                     WQEHQAGNAIAMLKKRLALEARALRDGKWQKIAARDLVPGDIVRVRNGDIIPADIKLV
                     EGDLLSVDESALTGESMPVEKHTSDIAYSGSAVKQGEMTALVVATGEKTFFGKTARLT
                     EEAMTASHFQKAVIRIGDYLIVLAIVLVTIVFIVSLFRQESVLDTLQFALVLIVAAIP
                     AALPAVLSITMAVGATALARKEAIVSKLVAIEEMAGVDILCSDKTGTITENKLTLAEV
                     APFEGFNDDDVLLAAILASREEDQDPIDIAIIGSKEAQGLKDRLEAYTVTKFKPFDAV
                     VKRTEATVHDGGGREFSVAKGAPQVILALAGGGKDLDEAIDRLSETFAKKGYRVLGVA
                     RSDTPGTWVYVGVLGLHDPPRADSAATIKTAEEMGLDVKMVTGDHVAIAREVAREVNL
                     KTNIVTAEAFLGKHDDEAAEAVEKADGFAEVFPEHKYRIVSLLQSRGHTVGMTGDGVN
                     DAPALKKANVGIAVAGATDAAKSAAAIVLTKPGLSVIIDAIKESRRIFQRMDHYVIYR
                     ITETINVLFFIALAILIFGFFPVTALMIVLLALLNDIPIMTIAWDNVLYSRSPEEWEM
                     RKTLTLATLMGITGVTASFLLLAIARGPLHLDFGVVQSFVFLKLMVAGHLTIFVARTR
                     GPFWSVRPGSALLWSTIGTKAIATLIVVYGIGVTPIGWPLAILVWIYSLIWEFGIMDP
                     VKIYTYRLIDHTGIQFAR"
     gene            complement(9486..11921)
                     /gene="ppsA"
                     /locus_tag="PLI76_03630"
     CDS             complement(9486..11921)
                     /gene="ppsA"
                     /locus_tag="PLI76_03630"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HOI61153.1"
                     /translation="MKQDATYIRWFIDVRNEDVGIVGGKNASLGEMYSELTPKGVKIP
                     DGFAVTADGYWHVLTSAGVLDDLKETLAGVDRSNVADLAKRGRKARDLILSAGIPDDL
                     WGEIKTAYDMLCDEYGPDADVAVRSSATAEDLPTASFAGQQETYLNIRGYQALREAYV
                     RCLASLFTDRAIAYRIDNHFDQFKVALSVGIMKMVRSDLASSGVIFTLDTDTGFRDVV
                     LITGSYGLGEMIVQGAVNPDEFYVFKPTFRKGYRAIVRKNLGEKRVKLIYGQGGAKEV
                     TRRVDVPDADQRRFCIADDDILTLAGYALTIEDHYSAKAGKPVPMDIEWAKDGETGEL
                     FIVQARPETVQSQKTGDVLETYRIDERGEVLAVGKSIGDKIATGKARVITDVGHLPEF
                     KPGEVLVSDTTTPDWEPVMRVAAAIVTNRGGRTSHAAIVSREHGIPAVVGTGDATEKV
                     PTGREVTVSCAEGEEGFVYDRVLPFHVEKTRLSDLRRPKTEVMMNLGNPDAAFGLAMV
                     PNDGIGLARMEFIISSYIKVHPMALVHPERVTDESVRQSIADLTYGYSNGEEYFVERL
                     AEGAGTIAAAFYPNPVVVRTSDFKTNEYASLLGGTYFEPAEANPMLGFRGASRYYDDR
                     YREGFALECRALKRVREEMGLANLIIMIPFCRRVEEAKQVIEELAKNGLSRGENGLQV
                     YQMCEIPNNVVQIDAFSDYFDGFSIGSNDLTQLTLGIDRDSAVIGGAFDERDPGVKKM
                     VAMAVEGCRRNKRHSGLCGEAPSTYPEFADFLVEQGIDSISVEPDAILKITLRVADVE
                     KRFAKKEMAPLPR"
     gene            complement(11918..12976)
                     /locus_tag="PLI76_03635"
     CDS             complement(11918..12976)
                     /locus_tag="PLI76_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOI61154.1"
                     /translation="MVKLVFFDLDEHEQDVVRDAFAREGGYEIDLHPDALTSKNASFA
                     RNADGIGVFVTSHVTREILDRLPNLKMVAAVSTGFDNIDVDACRERNIAVCNVPRYGD
                     NTVAEFAFGLILALARKFRPTFERTARGEFSRTGLQGMDIAGKTLGLIGTGHIGSHLA
                     RIAHGADMKIVAYDPHPKPDLTRDYGIRYLALDDLLRESDVISLHVPYTKATHHLINA
                     ERLQLVKNTAFLINTSRGGVVDTRAVATALNEGRLGGVALDTFEGEEVWIEEEFAGTG
                     EPPAAELKEALESFAILHSDRAILSPHNAFNTREALERILATSIENIRAFFAGNPQNV
                     VAGTYRVPAAPPAGGGRT"
     gene            complement(12985..13992)
                     /gene="gap"
                     /locus_tag="PLI76_03640"
     CDS             complement(12985..13992)
                     /gene="gap"
                     /locus_tag="PLI76_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOI61155.1"
                     /translation="MKKVAINGFGRIGRKVLSNYITDPPDNIAIVAVNDPNPTEELAY
                     LMKYDSVHGRAPFSIEAGSDRLKLGSKEVRVLHDRDPARLPWGDLGVDLVLECTGHFT
                     ERAGSAKHIDAGAKRVAISAPSPDADLTVVLGVNESAYDPAKHTIISNASCTTNALAP
                     AAKVLNDSFGVERLMATAIHAYTATQALVDRATKKPRRGRAAAVSLIPTTTGAAVATT
                     RVLPELTGKMDAIAIRAPIPDGSVVDIAAELKRETTTDAVNAAMKQAADGKMKGYLAY
                     TDDPLVSADIIGDPHSGVIDGRSTGVVDGRMARVLVWYDNEYGYAKRMVDIASYMASR
                     E"
     gene            complement(14264..15007)
                     /gene="gpmA"
                     /locus_tag="PLI76_03645"
     CDS             complement(14264..15007)
                     /gene="gpmA"
                     /locus_tag="PLI76_03645"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HOI61156.1"
                     /translation="MVELILLRHGESLWNRENRFTGWTDVDLSARGREEAHRAATLLG
                     GEHTFHVAYTSVLKRAIRTLWIVMDDLDLMYVPVQRSWRLNEKSYGALQGLDKPETAA
                     KYGTEQVHLWRRAYDVRPPPLSWDDSRHPRFDPRYADMDPETLPATESLHDTLNRVLP
                     YWESHITTDLRRGKPVLVAAHGNSLRALIKHLDNIPDGDIADLNIPTGYPLIYELDAD
                     LHPIRHYYLGDPEEVAAAARGVARQAGGR"
     gene            15238..16815
                     /locus_tag="PLI76_03650"
     CDS             15238..16815
                     /locus_tag="PLI76_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HOI61157.1"
                     /translation="MAPNRRRNKNYILATAALLIVVVAAIIAAGCTAGTSTPGAVIGL
                     TGVSWSLDSYCDENSTLVGVLQGTEITARFDDGKVTGLAGCNQYGGDYHQNGAALSFS
                     TLAQTLKYCEEPRGIMEQEARFMEVLGSTAGYRIENDRLLLTDAAGATVLVFTKEKPA
                     SIVGTSWTLAGLAGEDGGLLPVVPGTTVTAVFGADGRVGGSAGCNRYGANYAVHGADL
                     MIEPTTMTEMYCGEPSGVMEQEDRYLALLAEVASCRVEGDRLILTDANGTDILVFEPI
                     IRAPDLPLTGTDWVLDGYSTGKDAVSPVIAGTEITAMFAPDGKVAGSAGCNHYGGSYS
                     LDGGNLSVSSLFSTLMYCGEPEGIMEQEGKYLALLANVSSYRVQGDRLTLTDAEGTDL
                     LFFVMAEEVPPAPLAGTNWVLDTYSTGEDAVSSVIAKTTITVTFSPDGNIAGNAGCNN
                     YGAAYRLDGTNLSVSPPVSTKMYCGEPEGIMEQEARYLTLLESVAGYRIDGNRLDLLD
                     GAGGILLSFRADDADRS"
     gene            17007..17513
                     /locus_tag="PLI76_03655"
     CDS             17007..17513
                     /locus_tag="PLI76_03655"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HOI61158.1"
                     /translation="MRDGHHALAKAAFLIIVAACLVSAGYSGDTSPGDVGQAAEPLMG
                     TAWNLVGYRADNGSVVAPLPGKEPSVIFGGDGTLSVSAGCNLYSTSYRIDGSRIMVEP
                     ATAAGTHPAVEQDIARQESRYWELLLAAASYRVEGNRLVITGLSGQDLLAFSRAGEPA
                     DVPLLAME"
BASE COUNT         3151 a         5572 c         5492 g         3364 t
ORIGIN      
        1 tctcaccgac cctgtccggg ttccttgtag atgggtaccc aaggaactcg ccggcacccc
       61 cgccccgccc gagcctccag cgctcctccc ccgcctcctt ccggggacgg ggtccgtgcc
      121 cgggcgtata tcaggggagc agccgtggcc gggatgtatc tggtggaaag ccgtgccggg
      181 ttcttctccg ggatctccca tgtactggac cgtggtggca atgtcgcccc tgggggaggc
      241 ggcctgcggg gaagaactcg ccctctcgca gggctaccca tatgtttctc tctcctctca
      301 ccaccccggc ataacaatcc tcacgaccgc gagcgtcaca caggcgccga cctgaacggc
      361 cacgttatcc tcgagcggga agaacgcctc gacgatgccg gccgcggccg ctacaaccgt
      421 ggccgcccac cagggaataa ggaaggcgag cgcgagagcg accgcggcga ccccggtaag
      481 cgtcccctca agcggtttac tgtcccggag ggtgtgccgc ccgaaccgga gccccacgag
      541 cgtcgatatg ctgtcgagca tcccgaccgt gacgaggccg gccgcggcgt actcccgccc
      601 gaagacggcg agacagaaga gcgcgccgat ggcgcccttg agcggcacct ccccggtgcg
      661 ttccgactcg tcgagaatcc gcgagaggac cgggagatcg tagccgcgag tgaaggcgtc
      721 gcagacgaga aaccgggcga caagcaccgc gctcaggaat acgaaggccc ccggtcgtcc
      781 agacggagga cgatcccggc cccgatgagc cccatgagca ggtgggctgt ctggcggaac
      841 gtctcactca tcgcactgag atgacagccc caggagataa accaccgtgc ggatgccccc
      901 gggtgccggc ctattgccgg ggagcagtca ccttaaagta gtctccttct ctcctaccct
      961 cacgtgaggg agaagatcat ggtagaagta cgtaagtatg tcaacccgcc cgccgccgaa
     1021 gtgatatgcg agttccggtt tcccgaagag atcccctggg acctgaccta ccccggcatc
     1081 ctctacggcc acctgaaaga tacctacccg aaacgcgacc agcgctacgt ccgcgaggtg
     1141 gtgatgctcc tcggtcccga ggggctccgc gaggagctcc tggtcgccga gcggtcgata
     1201 ttcctcgctg aggacgaagg ttgtgccgtc caggtcgggc cccgcctgct atcggtgagc
     1261 tgccagaagc cttacgtcca ctgggaggcc ttctcggagc ggatccatgg ggcgttcgac
     1321 cggttccggg atgtcatcag cgccgatgcc gtcaggacca taaacctgcg ctacgtcaac
     1381 ctcatcgaga tcccggagcg ggaggtgacg ctctcggatt acttcgcctt ctatcccacc
     1441 cttccgccgg aactcccgcg gacgccggcg ggattcatca ccggatgcga gttcgcgttc
     1501 cacgacaacc gcgacaactg ccgggtggaa ctcaccgatg ctgtccctga atcgacgacg
     1561 aacaatgcgt tcctcttaaa catcgactac ttcctcacgg aggaggggag catcacgctg
     1621 gatggggtgg ccgactggct cgagatcgcc cacacccatg tgcgcgacat attcgaggcc
     1681 tgtatcaaag accccctccg cgacctcttc accatccgcg aggaggtccc ggcagcggca
     1741 cggtgatatc cgatgggcga cattgtgcgc aaaaaatgga tgacgatgat ggatgcccac
     1801 gcgggcgcag tcgatcaggt ctacggaggg ccggtcggtc tcatctggga gatgcttgcg
     1861 ggcgagggtg ccgacggcga taccggtgcc cttgcagagc gggcaggact cggccgggag
     1921 agccgggtgt tctccctcct ttccggaccg ggggaagcgg cccggcgcat cgcccggaag
     1981 tacggctgca ccgtgatcgg gcttgtgccg gtgccgcagg tggtcgacga ggcggtcagg
     2041 aagacggagg aggccggact tgccggccag gtcgactttc agcagggaaa cgcactcgat
     2101 atgtcgtttc acgatggagt cttcgatacc gtctggggtg aggacgcctg gtgctacgtc
     2161 atcgataagg accggatgat ccgcgaagcc ttccgggtct taaagccggg gggcaccctc
     2221 gcgttcaccg actgggtcga gatcggcccg atgagcgacg atgagtggcg caccccggag
     2281 accttccgga tcttccccta cttcgagacc gtcgaaaggt acgcggaact cctcgagcgg
     2341 aacggtcttc ggctcgtcga cggaggagag gttccgggcg accccatccg acaggtcggc
     2401 aatatccgca aacgactccg ggacagccgg gatgcgatca ccgaactcat cggtgaggag
     2461 gcctacgacg acgcccggcg agggctcgac ctctgggaga gagccgccgg tcgggggctg
     2521 atcgggcgcg ggtggctgct tgcagagaaa ccctgacccg gcacccctca tatcattccc
     2581 agtgtctccg tcacgatcgc cgggtttgca taccgcttct cgggcagcag atcgagcgct
     2641 cgcatgatct catccggtgc atgcgcgctt cgggcgtagt cgatcagttc ttcgcgggtt
     2701 accgggtagg gaacgtcgcg aaggtactcg tgaacgcggg agacggtcac gccgggtggt
     2761 aaagccgttc caatcacctc caatccctgt aatacaggga acctatttga ccatgccccc
     2821 gcgcggacgg gtacccgtcg ccggcacgag gccatatata gatgggaccc ggatagtcga
     2881 ataactcaag tccggagggt gaaagatgat ccaggtacgg ggaagcggca tactcctgca
     2941 catcacgtcg ctcccatcgg agtacggcat cggcgatttc ggaccggcgg cataccggct
     3001 cgtcgatgcc ctggctcaag cccggcagca ctactggcag atcctgccgc tcaatccgac
     3061 gcagacggaa tacgcgagct ctccctacag cagcccgtcg gcgttcggga tgaacaccct
     3121 cctcatcagc ccggatatac tcgtccgcga cgggttcctc aggcgggccg acctcgaccc
     3181 ggcacccgag ttcccggaga ggcgaacggt ctacgaggcg gtcgcatggt acaaggaccg
     3241 gatcttctcg gtcgcatacg agcggtttct tcattcgggg ccggagcgcg ggtatgaggt
     3301 gttctatgac aggaacgcat ggtggctgga cgaccacgcg ctctttgtcg cgctcaaggc
     3361 tcacttccgc gggcaggagt ggaaccggtg gccggaggag atccggggcc ggcagccgga
     3421 ggcccttgag gatatgcgcc ggcgccttgc cgataagatc cggaaagaga cgttcctcca
     3481 gtatcttgcc gcccggcaat ggtcggccct ccgccggtac tgcgccggcc ggggcatcca
     3541 ggtgatcggc gatataccga tctacgtcgc gtacgacagt gtcgatgtct ggcagaaccc
     3601 cgggatattc aaactggatg acaacctccg cccgaccgtt gtggcggggg tgcccccgga
     3661 tatgttcagc aagaccgggc agctctgggg gaatccgatc tacgactggg ccctcctccg
     3721 ggaccggggt tacgactggt gggtgaaccg gatcgcccgg gccggcgaac tctacgacct
     3781 cttccggatc gaccacttcc gggcgttcgc gaattactac gaggttccgg cgggcgacac
     3841 gacggccgag aacggcacat gggtcgacgg accgggaacc gatttctttg aggtgcttgc
     3901 ccggcggttt ccctgttttg ccatcgtcgc cgaggatctg ggggcaaaca cccccgccgt
     3961 ccaggagctg ctcgaccgat tcgggttccc ggggatgaag atcctgctct tcgcgttcgg
     4021 cgcggagctc cccgggagcg cccatgcccc tcacaactac gttccaaacc tcatatgcta
     4081 cacggggacg cacgacaaca acacggcccg gggatggttt gaggaagagg cgtcggagga
     4141 ggataaacag cggttctttg cctacatcgg gagggaggtg gcggcggatg aggtccaccg
     4201 ggaactcgtc cgcctcgcga tgacgtcggt tgcccgggcg agcatcaccc ccatgcagga
     4261 tcttctcgga ctcggtgccg cggcacggat gaactacccc tcgaccacca gcgggaactg
     4321 ggaatggcgc atgacccccg tggagtttgc cggtgctccg ctcgagtggc tccgcaacct
     4381 cacggtgctc tcgggacggg gatgaatcaa taccgggagg tcaggacctc gagctcttca
     4441 cggatggtcc ggcggatcat ctcctcgatg gcggtgtagt cgcgccttac cttctcctgc
     4501 ttcctgagca gttcttccat catcatgata ccggctgaga ggttcttccc gtacttcagg
     4561 gcgacctcca gcgcctcctc atcgacccgg acgactttcg acatgaaaga gtattttttc
     4621 tgtgcctgaa gttaaatgta acagttttgt tacacgaggg cgcccgatgt tacagatctg
     4681 taacagtacc ggatgtggtg taacatatgc gttacacgag gccggggtgg gaggttcacc
     4741 ccctccggcc gggatctcct tacaggtacg cctccgtcgc gtagcgccgc atcatccggt
     4801 gcgtattgaa gaagtaggcg atctttgcga tcgaacttcg catgatcgtc gcccactcat
     4861 cctcccagcg atagtaggtg gggacgatca ggtactcgag tttgctgtag agatcctgga
     4921 gtgcctccct cagccgctcg gcctcgctca cctttgcgcc aggtccggga ccgatcgccc
     4981 atcctgtcgt tccctcgatc cagccctcga tccaccaccc gtcgaggata ctgaagttga
     5041 ccacgccgtt cagagcagcc ttcatgccgc tcgtgccgct cgcctcgtag ggcgggaggg
     5101 gcgtgttcag ccagacgtcc accccggcgg tcatcaggcc cgcaatatcc atcgtgtagt
     5161 tctcgaggta gacgcccgtc acctccccct caaggtcgtg catggcgtcg tgaatcgcgc
     5221 ggatctcctt tttgccggca acatccgttg ggtgtgcttt gcctgaaaag acgagctgga
     5281 tcgctccctt cctgcttgcc tcccgcagtc tgtcaaggtc cgagaagagc agggttgccc
     5341 gcttgtaggc tgctgctcgc cgggcaaacc cgatcgtgag agcccggggg tccaggtcca
     5401 cattgttctt ctccttgaca tagttcacca gggcctgctt tgcctgctgg tgggcgtgcc
     5461 tgatctcctc gcgcgggatc gtatcgaccc tggcgagcaa ttccggttcc tgcgcccacc
     5521 cggagatgta gcggtcatag agattccgga acgggaccga tgtccacgta tgagggtgga
     5581 cgccgttcgt caccgcccgg atgtgaaacc cggggaagag ctgtcgcgag aagttcatgt
     5641 gcgccgtcgt cacgccgttg atgtacctgc tcaggcgaaa cgcaagggtc gcgagtgcaa
     5701 gcccgcccct ggcgatgtac ctgtcgagca gttccggcgg gatctcgccc tccagcaggt
     5761 ggaccaccat atcgcgcgga aacttctcga acgccaccgc caccggggta tgggtggtga
     5821 agatacagtg cgtctgcacc ttctcctcat ccatccccgg acgcctgagg agttcgaggg
     5881 tgaggagact tgagtggctc tcgttgatgt gatacttgcc gaccttgatg tccagagcgg
     5941 tgagcatccg ggctccgccg ataccgagca caatcgcctt tttgagccgg cattcctggt
     6001 cgccgccgta gagatgatcg gttatccccc ggtcttcggg cgcgttctca gggagatcgg
     6061 tatcgaggaa gagcaccggc acgaggccgc cgacagggct ccagtagtcg tagagccagg
     6121 cccggatcgt gacgttcgca tccccgatcc gcaccgtcac ccgccgggag agctcacgca
     6181 tgtgtttctt tggatcccat tcgtccggca ggtctatctg gtcgccctcc ggggtcaggc
     6241 gttggcgcac gtagcctttc cggttgacga gcgtcaccgc gaccatcggt atgaggaggt
     6301 cggcgctcga acggatgacg tccccggcaa gtacgccaag accgccactg taggtcggta
     6361 tatcgctctc aaggccgatc tccatggaga agtaggcgat cttctcgcca tcgacgaacg
     6421 tatccctgca gagctcgctt gagaagtagt aggtcacgcc acggacttcc accgagtagg
     6481 ctgcctttgc cgggtccacc ggcatcccgc agatggggtc cctcaccgcc atggccctgc
     6541 atcccccgga caccatatga acctgccgat accccggggc tcgggccggg ttatgcggca
     6601 cagcaccatc tgcatacacc tgcgacccga ttgccgccgg atatacgcgc ggacgactca
     6661 tccccgggtg ttcgagcgcc attcccgatc atcgcaggag aggagcgggg gaggccggca
     6721 gagaggcgcg ggcggggtaa gggtgctgga gaacgatgca cgggtatctc ccgtcgaagg
     6781 catgggccgg gggaggccgg cggggtgtat gggcatctcc gcagaaaggc cggcagggcg
     6841 gggaaccgga gaggaggagg gatatctgct ggaggatgtg gaggaggatc ctctacaggg
     6901 ggaaaaccca ccggaaaggc tgttctcatc tatccgtccc tccgccggct ttctcgcacc
     6961 gggccccggg gccggcagcg gcacttatat acgatcccgg tgagaggggg cgggcgggaa
     7021 gaccatggtg gagagccaga cgatcagcac cgataaagcc agaagtatgg ccgtcgacga
     7081 cctctaccag gccctctcct ccgggcaggg aggtctcggc caatccgagg cggagagccg
     7141 catccggaag ttcggcccca acgagatcgt cgagaaagag gagagcgaga tcctgaagtt
     7201 cttccgctac ttctggggcc ccatcccctg gatgatcgag gcggcggtca tcatatccgc
     7261 catcatcagg cactgggagg ccttcgcggt catactcgcc ctccttctca tcaacgcgat
     7321 cgtcggtttc tggcaggagc atcaggcggg caacgccatc gccatgttaa agaagaggct
     7381 cgcactcgag gcccgggcgc ttcgcgacgg caagtggcag aagatcgccg cacgagacct
     7441 cgttcccggg gacatcgtcc gggtcaggaa cggagacatc atacccgcag atatcaagct
     7501 cgtcgaagga gacctcctct cggtcgatga gtcggccctc accggcgagt cgatgccggt
     7561 cgagaagcat acatcggata tcgcctactc cggttccgcc gtcaagcagg gtgagatgac
     7621 tgcgctcgtg gtggccacgg gagagaagac cttctttggc aagaccgccc ggctcaccga
     7681 ggaggcgatg acggcgagcc actttcagaa ggccgtcatc aggatcggcg actacctgat
     7741 cgtgctcgcg atcgtcctcg tcaccatcgt cttcatcgtc tcgctctttc ggcaggagag
     7801 cgtccttgat accctccagt tcgcactcgt cctgatcgtt gcggcgattc ccgccgcgct
     7861 gccggccgtc ctctcgatca ccatggccgt gggggcgacg gcacttgcca gaaaagaggc
     7921 catcgtcagc aaactcgtcg caatcgagga gatggccggt gtggatatcc tctgctcgga
     7981 caagaccgga acgatcaccg agaataaact taccctcgcc gaggtcgccc ccttcgaggg
     8041 gttcaacgat gacgatgtac tccttgccgc catcctcgca tctcgcgagg aggaccagga
     8101 ccccatcgat atcgctatta tcggctcaaa agaagcgcag ggcctcaaag atcggctcga
     8161 agcatatacc gtcaccaaat tcaagccttt tgatgcggta gtaaaacgca ccgaggccac
     8221 cgtccatgat ggtggcggcc gggagttctc ggtcgcaaaa ggtgcaccgc aggtgatcct
     8281 cgccctcgcc ggcgggggaa aggatctcga tgaggcgatc gaccgtctct ccgagacctt
     8341 cgcaaagaaa ggctaccggg ttctcggcgt tgcacggagc gacacccccg gtacctgggt
     8401 atacgtcggc gtcctcggtc tccacgaccc gccccgcgct gattccgcgg caaccatcaa
     8461 gacggcggag gagatgggac tcgacgtcaa gatggtcacc ggcgaccacg tggcgatcgc
     8521 ccgcgaggtc gcccgcgagg tgaacctcaa gaccaacatc gtcaccgccg aagccttcct
     8581 cgggaagcac gacgacgagg ccgcagaggc cgtcgagaag gcagacgggt ttgccgaggt
     8641 cttccccgag cataaatacc ggatcgtctc cctcctccag tcccggggac acaccgtcgg
     8701 catgaccggc gacggcgtga acgatgcccc cgccctgaag aaggccaatg tcgggatagc
     8761 ggtcgccggg gccacggatg cggcgaagtc cgcggcggcg atcgttctca cgaaacccgg
     8821 gctctcggtg atcatcgatg cgataaaaga gagccggagg atctttcagc gcatggacca
     8881 ctacgtcatc taccggatca ccgagaccat caacgtcctc ttcttcatcg ccctcgcgat
     8941 cctgatattc gggttctttc cggtcaccgc cctgatgatc gtcctcctcg ccctcttaaa
     9001 tgatatcccg atcatgacca tcgcctggga taacgtcctc tactcccggt cccccgaaga
     9061 gtgggagatg cgcaaaaccc tcacgctcgc gacgctcatg gggatcacgg gcgtcaccgc
     9121 ctcgtttctc ctgctcgcca tcgcccgggg gccgttgcac ctcgatttcg gggtcgttca
     9181 gtcgttcgtc ttcttgaaac tcatggttgc cggccacctc accatcttcg tcgcccgcac
     9241 ccgtggcccg ttctggtctg tccggcccgg atcagccctg ctctggtcga cgatcggcac
     9301 aaaagctatc gcgaccctga tcgtcgtcta cgggatcggc gtaacgccca tcggctggcc
     9361 gcttgcgatc ctcgtctgga tatactcgct catctgggag ttcgggatca tggatccggt
     9421 gaagatctac acctaccgcc tcatcgacca taccggcata cagtttgccc ggtgagggat
     9481 atccctcacc ggggcagcgg tgccatctcc ttctttgcga accgcttctc gacatcggcc
     9541 acgcggaggg tgatcttcag gatcgcgtcg ggctcgaccg atatcgagtc gatcccctgc
     9601 tcgacaagga aatcggcgaa ctccgggtag gtgctcggcg cctcgccgca gaggccgctg
     9661 tgccgcttgt tccgtcggca tccctcgacg gccatcgcga ccatcttctt gacgccaggg
     9721 tcgcgctcat cgaacgctcc ccctatgaca gcggagtcgc ggtcgatgcc gagcgtcagc
     9781 tgggtcaggt cgtttgagcc gatcgagaat ccgtcgaagt agtcgctgaa cgcatcgatc
     9841 tggacgacgt tgttcggtat ctcgcacatc tggtagacct gaagaccgtt ctcgccccgc
     9901 gaaagaccgt tctttgcaag ttcctcgatg acctgcttcg cctcctccac ccggcggcag
     9961 aaggggatca tgatgatcag gttcgcgagt cccatctcct ctctgacccg cttcagcgcc
    10021 cggcattcaa gtgcaaatcc ttcccggtag cgatcgtcat agtagcggga ggcgccgcgg
    10081 aacccgagca tcgggtttgc ttccgcgggc tcgaagtaag tcccgccgag cagacttgcg
    10141 tactcgttcg tcttgaagtc gctcgtgcgg acgacgacgg ggttcgggta gaacgccgcg
    10201 gcgatggttc ccgcaccctc cgcgagcctc tccacgaagt actcttcgcc gtttgagtag
    10261 ccgtaggtga ggtcggcgat cgattgacgg acggattcat ccgtaacccg ttcggggtgg
    10321 acgagcgcca tcggatggac cttgatgtaa ctgctgatga tgaactccat ccgggcaagc
    10381 cctatgccgt cgttggggac catcgcaagc ccgaatgccg catccgggtt cccgaggttc
    10441 atcatcacct cggtcttcgg ccttcgcagg tccgagagcc gggtcttctc gacgtggaac
    10501 gggaggactc gatcgtagac gaacccctct tcgccctcgg cgcagctgac cgtcacctcc
    10561 cggccggtcg ggaccttctc ggtcgcgtcg cccgtgccga cgaccgcggg gatgccatgt
    10621 tcccggctga cgatcgcggc gtgggaggtc cggccgcccc ggttcgtcac gatggcggcg
    10681 gcaacccgca tcaccggctc ccagtcgggg gtcgtggtat ccgagaccag aacctcgccc
    10741 ggtttgaatt ccgggaggtg gccgacatcg gtgatgaccc gggccttccc ggtggcgatc
    10801 ttatcaccga tactctttcc gacggcaagc acctccccgc gttcgtcgat acggtaggtc
    10861 tcaaggacat ctcccgtctt ctgcgactgc accgtctcgg gtcgcgcctg gacgatgaag
    10921 agttccccgg tctctccgtc ttttgcccac tcgatatcca tcggcaccgg ttttccagcc
    10981 tttgccgagt agtggtcctc gatcgtgagc gcataccctg caagcgtcag gatatcatcg
    11041 tcggcgatac agaaccgtct ctggtcggca tccgggacat cgacacgccg ggtgacctcc
    11101 tttgcacccc cctggccgta gatcagtttc acccgcttct caccgaggtt cttcctgacg
    11161 attgcccggt aacctttccg gaacgtcggt ttgaagacgt agaattcgtc ggggttgacg
    11221 gcaccctgga cgatcatctc gccaagaccg taggatccgg tgatgaggac gacgtccctg
    11281 aaaccggtgt cggtgtcgag cgtgaatatg accccgcttg acgcgaggtc ggagcggacc
    11341 atcttcatga tcccgaccga gagggcgacc ttgaactggt cgaagtggtt gtctatccgg
    11401 taggcgatag cccggtcggt gaagagagag gcaaggcatc tgacgtaggc ctcccgcagc
    11461 gcctggtagc cgcggatgtt taagtaagtc tcctgctggc ctgcaaacga ggccgtcggg
    11521 agatcctcgg cagtggccga actccggacg gcgacatcgg cgtcgggccc gtactcgtcg
    11581 cagagcatat cgtaggccgt cttgatctcg ccccagaggt catcgggtat cccggccgag
    11641 aggatgaggt cacgggcctt ccttccccgc ttcgcgaggt cggcgacgtt gctcctgtca
    11701 acccccgcga gggtctcctt caggtcgtcg agtacccctg cggacgtgag gacgtgccag
    11761 taaccatcgg cagtcacggc aaatccgtcg ggtatcttca cgcctttcgg tgtcagttcg
    11821 ctgtacatct cgccgagcga tgcgttcttc cccccgacga tgccgacgtc ctcgttcctg
    11881 acgtcgatga accagcggat gtaggttgca tcctgcttca tgttcttcca cctccggcag
    11941 gcggcgccgc cggcacccgg taggtcccgg caacgacgtt ttgggggttt ccggcaaaga
    12001 aggcccttat gttctcgatg ctggtcgcaa gtatccgctc aagcgcctca cgggtgttga
    12061 aagcgttgtg cggcgagagg atcgcccggt ccgagtgcag gatggcgaag ctctcgagcg
    12121 cctccttgag ctcggccgcc gggggttcgc ccgtcccggc aaactcctcc tcgatccaga
    12181 cctcctcgcc ctcgaaggtg tcgagcgcga cccccccgag gcggccttcg ttgagggcgg
    12241 tcgcgaccgc ccgcgtatcc acgacaccgc cccgggatgt gttgatgaga aacgctgtgt
    12301 tcttgaccag ctgcaaccgt tcggcgttga tgaggtggtg cgtggccttc gtgtagggga
    12361 cgtgcaggct gatgacgtcg gactctcgca gaaggtcgtc gagggcgagg taacggatgc
    12421 cgtagtcccg ggtgaggtcg ggtttcgggt gtgggtcgta ggcgactatc ttcatatccg
    12481 ccccgtgggc gatgcgggca aggtgcgatc cgatgtgccc ggtcccgatc aggccgaggg
    12541 tttttccggc gatgtccatg ccctgaaggc cggtccggga gaattcgccc cgggcagtac
    12601 gctcaaacgt cggcctgaac ttccgggcaa gggcgaggat gagcccgaac gcgaactccg
    12661 ctacggtgtt gtcgccgtaa cgcgggacgt tgcagacggc tatgtttctc tcccggcagg
    12721 catccacgtc gatattgtca aacccggtgg agacggcggc gaccatcttc aggttcggca
    12781 accgatcgag gatctcacgg gtgacgtgcg atgtgacaaa gacccctatc ccatcggcgt
    12841 ttcgggcaaa ggacgcgttc ttgctcgtga gcgcatcggg gtgcaggtcg atctcatagc
    12901 cgccctcgcg ggcgaaggca tccctgacca catcctgctc gtgctcgtcg aggtcgaaga
    12961 agaccagttt taccatgggt cccctcactc ccgtgaagcc atgtaggagg cgatgtcgac
    13021 cattcttttt gcgtacccat actcgttgtc gtaccagacc aggacccggg ccatccgccc
    13081 gtcgacgacg cccgtggatc ggccgtcgat gaccccggag tgcgggtcgc ctatgatatc
    13141 ggccgatacg agcgggtcat cggtgtaggc gaggtagccc ttcatttttc catccgcagc
    13201 ctgcttcatc gcggcgttca cggcgtcggt ggtcgtctct cgcttcagtt ctgcggcgat
    13261 atcgacgacc gacccgtcgg ggatgggggc ccggattgct atcgcatcca tctttccggt
    13321 gagttcgggg aggacgcggg tggtggcgac ggcggcgccg gtcgtcgtcg gtatgagcga
    13381 gacggccgcg gcacgtcccc ggcggggctt cttcgttgcc cggtcgacca gcgcctgggt
    13441 agcggtgtag gcatggatcg ccgtcgccat cagccgctcg acgccgaacg agtcgttgag
    13501 gacctttgcc gccggtgcga gcgcgttggt cgtgcaggag gcgttggata tgatagtatg
    13561 ttttgcgggg tcgtaggcac tctcgttgac gccgagcacg accgtcaggt ccgcgtcggg
    13621 cgagggtgcg ctgatggcga cccgtttcgc cccggcatcg atgtgttttg ccgagcccgc
    13681 ccgttcggtg aagtgtccgg tgcactcaag caccagatcc acgccaagat cgccccacgg
    13741 aaggcgtgcg ggatctctgt cgtgcagcac ccgcacctct tttgacccca atttgaggcg
    13801 atccgaaccg gcctcgattg agaagggcgc ccgcccgtgg accgagtcgt acttcatcag
    13861 gtaggcgagc tcttctgtcg ggtttgggtc gtttaccgcg acgatcgcga tgttatccgg
    13921 cggatcggtg atatagttgg agaggacctt ccgcccgatc cgcccgaacc cgttgatggc
    13981 aaccttcttc atgacacttc ccccctgtca gacaggtggt ctcccctcta caggatggta
    14041 tatatgatct tctaccaccg ggttctgccc cggaaagtga gcgaaggtgt cgcctcttca
    14101 tgaaaaatct catttacact ggagatgggt tcccggctgg tgcgttcccg ggcacggttc
    14161 gtcctgccgg atatcgccat gactgccccc ccttccccca tcggggcgag aggtgagagg
    14221 gaggaggtta tcaacctctc ctgcggggag gggccacccc cggctatctt cctcccgcct
    14281 gccgggcaac accccgggcg gccgctgcga cctcctcggg gtcgcccagg tagtagtgcc
    14341 tgatagggtg caggtcggcg tccagctcgt agatgagggg gtagccggtg gggatgttga
    14401 gatctgcgat atcgccgtcc gggatattgt ccaggtgctt gatcagggca cggaggctgt
    14461 ttccatgggc tgcgaccagg acgggttttc ccctccggag atccgttgtg atatggctct
    14521 cccagtacgg gagcacgcgg ttgagcgtgt cgtgcaggga ctcggtcgca gggagggtct
    14581 ccgggtccat gtcggcatag cgggggtcaa accgcgggtg ccgggagtca tcccatgaga
    14641 gcggcggcgg ccgaacgtcg tatgcccggc gccagaggtg aacctgctcc gtgccgtatt
    14701 tcgcggcggt ctccggctta tccaggccct gcagggcgcc gtaactcttc tcgttcaacc
    14761 gccatgaacg ctgcaccggg acgtacatga ggtcgaggtc gtccatgacg atccagagtg
    14821 tccggatggc ccgtttcagt accgatgtgt aggcgacatg gaatgtatgc tcgccgccaa
    14881 ggagcgtcgc cgcccggtgg gcctcttctc tgccccgggc cgagaggtcc acgtcggtcc
    14941 agcccgtgaa ccggttctcc ctgttccaga ggctctcgcc gtgccgtaag aggatcagtt
    15001 ctaccattgc gtatctctcc catacgagga ggcatccacc gggcctccac ccatcgtggt
    15061 agtcctccat accggatata atcgcccccg cctccccctg gcaggggcgg ggcataggag
    15121 gccggagccg atataacctc tataaacagg ccgtttaaca aatcgggctc ctataagttc
    15181 gatttttttc gtagcttacc atccatttat ccctcaatcg ctcccatatg gtagcgtatg
    15241 gcaccgaaca gaagaaggaa caagaactac atactcgcaa cggcggcgct cctcatcgtc
    15301 gtcgtggcgg caatcatcgc cgcggggtgc acggcgggca cgtcgacacc gggcgctgtc
    15361 atcgggctca ccggggtctc ctggtcgctt gactcctact gtgatgagaa cagtacactc
    15421 gtaggagtcc tgcagggaac ggagatcacc gcccggttcg acgacggaaa ggtcacgggc
    15481 ctggccgggt gcaaccagta cggcggcgac taccaccaga acggcgcggc tctctcgttc
    15541 tccacgctcg ctcagaccct gaaatactgt gaggagccac ggggcatcat ggagcaggaa
    15601 gcacggttca tggaggtcct cgggtcgacg gcgggctacc ggatcgagaa cgatcggctc
    15661 ctcctcaccg acgctgccgg ggcaacggtg ctcgtcttca cgaaggaaaa acccgcgtcg
    15721 atcgtcggaa catcctggac gctcgccggc ctcgccgggg aggatggcgg tctgctaccg
    15781 gtcgttcccg gcacaacggt cacggcggtc ttcggtgccg atggcagagt cggcggatcg
    15841 gccggatgca accgctacgg tgcgaactac gccgtacatg gcgcggacct catgatcgaa
    15901 ccgaccacca tgaccgagat gtattgcggc gaaccctccg gcgtcatgga gcaggaggac
    15961 cgctaccttg cgctcctggc cgaagtcgcc tcctgccggg ttgagggcga ccggctgatt
    16021 ctcaccgacg ctaacggcac cgatatcctt gtctttgagc cgatcatccg ggcgcccgac
    16081 ctcccgctta ccggaacgga ctgggtgctc gacggctaca gcaccgggaa agatgcagta
    16141 tccccggtga tcgccgggac agagatcacc gctatgttcg cgcccgacgg gaaggtcgcc
    16201 gggagtgccg ggtgcaacca ctatggcggc tcgtacagcc tcgacggcgg gaacctctcg
    16261 gtctcgtcgc tcttcagcac ccttatgtat tgcggcgaac cggagggcat catggagcag
    16321 gaggggaagt acctcgcgct ccttgcaaac gtatcgtcct acagggtaca gggcgaccgg
    16381 ttgaccctca cggatgcaga ggggaccgat ctcctcttct ttgtgatggc ggaggaagtc
    16441 ccgccggcgc cgcttgccgg cactaactgg gtgctcgaca cctacagtac cggagaagat
    16501 gcagtctcct cggtgatcgc taaaaccacg atcacggtga cgttctcacc ggacggcaac
    16561 atcgccggga atgccgggtg caacaactac ggagccgcct accggctcga cggcacgaac
    16621 ctttcggtct cgccgccggt cagcacgaag atgtactgcg gcgaaccgga ggggatcatg
    16681 gagcaggagg cgcgttacct cacccttctt gagtccgtgg caggctaccg tatcgatggg
    16741 aaccggctcg acctccttga cggggctgga gggatcctcc tttccttccg ggccgatgac
    16801 gcggatcggt cctgacccga cctccgtccc ctttttccac gtataccgct cgaggacggt
    16861 ttttttgccc gccggaacca tatccggatg agggctacaa aacgacgcct cctctgcaag
    16921 cgctcaagaa agatggcgat cgagagaatc tcgatgaaaa tcggttattt tttcttccct
    16981 cagcccttac gcctaccgtg gtaaatatga gggacgggca tcacgcactt gcgaaggccg
    17041 cattcctgat catcgtcgcg gcgtgcctcg tctctgccgg gtactccggc gatacatctc
    17101 ccggcgatgt cggtcaggcc gccgaaccac ttatgggaac ggcgtggaac ctggtcggat
    17161 accgtgccga taacggctcc gtcgtcgcgc ccctgcccgg gaaggagcca tccgtcatct
    17221 tcggcggcga tggcacgctc tcggtgtccg ccgggtgcaa cctctactcc acctcctacc
    17281 ggatcgacgg ttccaggatc atggtggagc cggccaccgc cgcaggcaca caccctgcgg
    17341 tggagcagga tatcgcccgg caggagagcc ggtactggga actccttctt gcggctgcat
    17401 cataccgggt tgagggcaac aggctcgtga tcaccgggtt gtcgggccag gatctgctcg
    17461 ccttctcgcg ggccggcgag cctgcggatg tgccgctgct cgcgatggaa tagcggccgg
    17521 ctacagcacc aacgacacat cgatgccgcg cgcggagcac atgttctatt cggggggcg
//
LOCUS       DAOSEE010000029        17468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1324_length_17468_cov_220.086200, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000029 DAOSEE010000000
VERSION     DAOSEE010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 17468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17468
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1324_length_17468_cov_220.086200"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PLI76_03660"
     CDS             complement(<1..238)
                     /locus_tag="PLI76_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61159.1"
                     /translation="MVKRSSYVAPTPWAAQPIGICTNLRGEDIPGHRRATAEYPARMR
                     KQRERKKGWGAYFSSSASRIVNPMTLSPSSSFMIR"
     gene            289..864
                     /locus_tag="PLI76_03665"
     CDS             289..864
                     /locus_tag="PLI76_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2178 domain-containing protein"
                     /protein_id="HOI61160.1"
                     /translation="MKQQTYRICIVLVAVGLFFALGLSLVLTSANMIVPAVAVAVSIA
                     ILALCRRNVTEVIEDELSTILHGKAALSAFEVTILIAAIGFAVLMIFSFTGGSGSSLS
                     RCENGSILVVYGVLSAPPNPEFLYENTYLIPDPANLTIDDVVALDDLFAEGHRIRDTT
                     RAFGAALGAITVLLTGLYGAFLWHYNRKYGA"
     gene            870..1088
                     /locus_tag="PLI76_03670"
     CDS             870..1088
                     /locus_tag="PLI76_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOI61161.1"
                     /translation="MKNKIKVFRAMHDMTQEELADRIRVTRRTINSIERGKYNPSIEV
                     AYNIAKTFGVAIEEIFCFEDDEDAEARR"
     gene            1085..1519
                     /locus_tag="PLI76_03675"
     CDS             1085..1519
                     /locus_tag="PLI76_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOI61162.1"
                     /translation="MILCDRLTKVYNGIPAVDDLCLEIPAGEGMNQNIYIMQKYICTC
                     KMITTSTIPELSKLLAFLGNEQRLRILKSIADEEKYAREISEELSISRTLVNIYLKQL
                     ERMGLVTGTSRVTDDPPLMRRYYRAVPFELVVSLDAIKKTEE"
     gene            1523..1888
                     /locus_tag="PLI76_03680"
     CDS             1523..1888
                     /locus_tag="PLI76_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61163.1"
                     /translation="MEHSNARGFHWLTWTVIGLLALLLIGLLVTIVYQPNLGIVVMFM
                     AGILQYVFLLAFIGIVVCIFGGRLKAWLEKYLDALVEQKSKDTDAGAKLTVLNEKIGE
                     MEGKIDRIEAVLVKISEQE"
     gene            1894..2346
                     /locus_tag="PLI76_03685"
     CDS             1894..2346
                     /locus_tag="PLI76_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61164.1"
                     /translation="MELGQNRNRDWIAWAAVAITAWLVMSILVALANPVVARWIWPGL
                     LPVFILAYGCLAVCYLVKRVKGWSERYLDTLLEQQTFGNEEEVRKIQAMVEHVGEMEK
                     KVDRIEAMLIMDGDREPEIEVQEGTSANPPLPKGTHESPGSGLPYDPL"
     gene            2333..3244
                     /locus_tag="PLI76_03690"
     CDS             2333..3244
                     /locus_tag="PLI76_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI61165.1"
                     /translation="MILCDRLTKVYNGIPAVDDLCLEIPAGEVFGLLGPNGAGKSTTI
                     LMLVGLIRPTSGACSIGGIEVAAHPIEVKRKIGYMPEDVGFYADRTAAENLDYAARFY
                     GLSEAERKRRVGDLLALVGLDGVGTTVGGYSKGMRQRLGLARALVNDPEVVILDEPTA
                     NLDPRGVLDYRRIVRDLAGRGTTVLVSSHILSEVSKVCTSVGILARGRLIASGDREVL
                     SRAAMQDRVTIDVETGTPMPRFASPEILSAEFSQDACSARIVAESDISTLIAKTLYAE
                     GILLRRLAVEKPDMEDILLSYYTEGTA"
     gene            3241..4305
                     /locus_tag="PLI76_03695"
     CDS             3241..4305
                     /locus_tag="PLI76_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOI61166.1"
                     /translation="MTRTALRIIAGKEFSDHLRSRRFHLLFAILAVVAAVGMITGAVQ
                     YTRDLSDYNAVQMIAEGEDDPTLAGNLAGLMRPSILSAYSQMGMLMAGIGVVLGIAMG
                     FDLVTREKESKSLKSLLAYPVFRDEVINGKALGGVGAIALAMGAVLVVSVAVMALFGI
                     VPDLGEFVRILVFGTASFLLVFTFFALALLMSTVSKDSGNALLYSLIVMIVLSSFVPI
                     FAYTPVYSAVFGDPPAQPGTATYSYQQGSSAYMVTTTKVGVRASGDAIDPEALAEYER
                     ASREYTNQRRTITDIVTLVSPTGNYQTILGAASQPAGDNTPGLGSLLGALAYNLIALL
                     AMPAAFFGLAWVRFMREDIR"
     gene            complement(4459..4704)
                     /locus_tag="PLI76_03700"
     CDS             complement(4459..4704)
                     /locus_tag="PLI76_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61167.1"
                     /translation="MVYRWNDRRDEAVVEMNAPDAGQAICGWLMHALQQAIYDSGPEP
                     AGYFFRELERHNPASLRCFREPEFWPSGGCSANPCGT"
     gene            4834..5247
                     /locus_tag="PLI76_03705"
     CDS             4834..5247
                     /locus_tag="PLI76_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOI61168.1"
                     /translation="MFHTTIELETHGEGEITDLTFQVQQAITESAVRMGLVTVFVTGS
                     TAAVTTIEYEPGVLSDLSRALAVIAPDDIPYAHDAAWGDGNGRSHVRAAIVGPSLSIP
                     VIGGRLACGAWQQIVLLELDVRASRRRTVHVTVHD"
     gene            complement(5388..5651)
                     /locus_tag="PLI76_03710"
     CDS             complement(5388..5651)
                     /locus_tag="PLI76_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0147 family protein"
                     /protein_id="HOI61169.1"
                     /translation="MASPDETIRICIQMLQNISEDSTIPRNIRRVADETKTVLLDNSR
                     SIGLRAATAISMIDEISNDPNMPVHARTRIWELVSQLETVPLD"
     gene            5718..6068
                     /locus_tag="PLI76_03715"
     CDS             5718..6068
                     /locus_tag="PLI76_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sjogren's syndrome/scleroderma autoantigen 1
                     family protein"
                     /protein_id="HOI61170.1"
                     /translation="MTADKADEVMAEYLLKGGKMLAKSCKVCGYPLFEYRGETRCVVC
                     PLAEPESPLPVPEPAQSPSPAREPPGPVAAEIERTIVHLCERIRSEPRAGECLTLMKA
                     VDRGARALGRLNQR"
     gene            complement(6059..8344)
                     /locus_tag="PLI76_03720"
     CDS             complement(6059..8344)
                     /locus_tag="PLI76_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOI61171.1"
                     /translation="MTSVSHPLIRPESIEERRYQLAIALRALDANTMVVLPTGLGKTA
                     VALLVAASRLYSHPGKVLVLAPTKPLVEQHLRFFKRFLLFREASEPEESDFAMFTGDT
                     SPDDRAKAWEACRICFATPQVIKNDCLAGRYSLADVVLLVVDECHRAVGNYAYVFLAR
                     HYCTTAQKPLLLAMTASPGGDRMKVQEVCANLHVEAVETRVETDEDVRPYIHERDIQY
                     VDVYLPEELQAALVALRGLVEARLTRLASLGFQVPKPDKLSIKALNLLNAQIQQRIQA
                     RDPAAFAAASLHAECMKLRHAISLAETQGSEALKLYLARLGAEGASGAGSRASKHLIG
                     DPAYQRLVEVAGGWKEELHPKVPIVCEIVRAQLAAHPDSRIIVFATYRDTVQTLVDAI
                     SASGVACERFVGQASRDAERGLSQKEQIASLSRFREGEFRCLVATSVGEEGLDVPSTD
                     MVIFYEAVPSEIRSIQRKGRTGRSGSGTIVVLVTKGTSDETFRYVSQTRERAMVKGIR
                     DMSVAPVPVPDSISTLTPVPAPAPSIPAATRQADILAFASSGPAIVVDDREMSSRVIE
                     RLHELGASITIERLDLGDYAIGDRILVERKTVQDFVDTLIERDLFGQVGAMADAVPRP
                     VLIVEGGDDLYGVRNLHPNAVRGTLAAITVGMGVTLIRTRDADDTAGMLYVLAQREGG
                     ERGERKVHPKKSHRSVREEQEYALAAFPNIGLKSARLLLEHFGSLKAIFDADAEDLAS
                     VHGVGEKTARGIWELARRPYR"
     gene            complement(8341..10071)
                     /gene="glyS"
                     /locus_tag="PLI76_03725"
     CDS             complement(8341..10071)
                     /gene="glyS"
                     /locus_tag="PLI76_03725"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOI61172.1"
                     /translation="MAETSDIYEKVMDVARRRGFVWPSSEIYGSVAGFIDYGPLGAML
                     KRNIENLWRSFYVFQEGYYEIECPTVGTEAIFVASGHVKEFADKMVQCPHCGEYLRAD
                     HIAAENGIENAGTLSAAALQAALYELPCPACKECLGEAGVFAFNLMFETTIGPGSQRR
                     GYLRPETAQGIFTDFPRLLRFYRDRLPFGAVQIGKSYRNEISPRQGMIRLREFSQAEA
                     EIFVHPDEKDHPAFYRYADHTAPLLTIARQVENLEPAAITMRAAVDEGVIANEYVAYY
                     IALTHELLLAIGVDPSRLRFRQHLTDERAHYAADCWDAEVYSGRFGWVEIVGIADRTN
                     YDLRSHAAHSGTSMTVFVPYDEPRRVVKRRIVADMGVLGPRFRGKAKAIADALAASDP
                     GEDGARVTVDGEEFFIPADLYQVREEEVEVRGEEVMPHVIEPSYGIDRMIYVALEHSY
                     AEDEVDGEVRKVLRLPPTVAPVQAAVFPLMNRDGLDGIAQAITDRLTRCRILAQYDDS
                     GAIGRRYRRQDEIGTPFAVTVDYDTLEDNTVTVRDRDSMEQVRVPVERLPEVLSALIS
                     GAAAFRELRV"
     gene            10383..10991
                     /locus_tag="PLI76_03730"
     CDS             10383..10991
                     /locus_tag="PLI76_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator receiver protein"
                     /protein_id="HOI61173.1"
                     /translation="MAEDTRKQQLLELFTTITNKKTIVEPMRKVHGTLRDRDAVEREI
                     ALIMREVLDQGYFKTKLAPRQLARLVVAYYDGKNDTEIARALGDEKLSKTVARARVRL
                     KLFRDLDFKMPFDRGMMTDLLDSGKTMKEISEELDVSPSTLREYRHVIEQERDTTLDP
                     YLERIRDVMEDRDLSEQMTRGVANDGLSEAIDIAEAIDMGEF"
     gene            11058..11726
                     /locus_tag="PLI76_03735"
     CDS             11058..11726
                     /locus_tag="PLI76_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOI61174.1"
                     /translation="MRLTWLGHACFLLSGSKTILIDPFIPEGTLPVEPDIVAVTHAHA
                     DHLGMTVQLGKKTVAVNEVAKYLAAKGLPVEPMNIGGSITVDGVRFTMTPALHSSWLE
                     NEGMGCYGGVAGGFVVTMDGTRIYHAGDTGLFSDMQLIRDLYWPDVALLPVGGRFTMG
                     PDEAMIAARYIGAPLVIPMHYDTFPAIRQDLEGFKRAIERTTSIRVAVITPGESIEVG
                     REGA"
     gene            complement(11966..12931)
                     /locus_tag="PLI76_03740"
     CDS             complement(11966..12931)
                     /locus_tag="PLI76_03740"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HOI61175.1"
                     /translation="MEENMFTPGGRKRLQAKSDRENAPYPRGDGKVKLVTPAWLADHM
                     DDADLTIIDTQPNVHDYIQEHIPGAVYLTEGVLRVSNRGFPTSYSPDACIQESFRRAG
                     LKADSPAVVYTGKGVFSGWGDGLAQVMMAYTLAKYGHDTVYILDGGIDGWKSEGKELS
                     QEFPSVEPSGFTVNVRNDYAVGYDEFQKIKDNDDVIVLDARPKAFYEGTSGPWRARGH
                     IPGAISLPWRSLMDDANPALFKSNDELDTILEGHGVDRFRTVICTCGTGREATNEFVL
                     LKWLYRYPNVRLYEGSITEWTRYPDNPVVTGPRAREERTEAAPAR"
     gene            13229..14203
                     /locus_tag="PLI76_03745"
     CDS             13229..14203
                     /locus_tag="PLI76_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HOI61176.1"
                     /translation="MKKIIVYVRKDGRDRVEEALKGLRYTVSLVQDIYEIVVYTPEEN
                     LDDLVGRLRDVLDLQHDENMIEVSTPDFVVSSLLLHAEQEEGRKEEKTPVEELIDSVK
                     GYAELDLEKVALTSIAGLIALSGLFLDNPVIIIGAMLLSPIIGPIYGFAIYTALGMIR
                     ESLKCLGVLSALLLGVFLLSVMATFLIGSAMPLALTDEITSRLVANPIYTLVAVLLGF
                     VAVLAFNRGTSEVIAGVAIASAIIPPTVVAGLVLVLQPMSLVSSALLVAGQIAGLITG
                     ALVAVTLQKIEPRETRDRTVARRHLRWSVTLIVLLFALLLGITWLMWR"
     gene            14320..15330
                     /locus_tag="PLI76_03750"
     CDS             14320..15330
                     /locus_tag="PLI76_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HOI61177.1"
                     /translation="MEIRKVQITGGSSYIVSLPKQWIRSTKIQKNDPVGLIVQPDGSL
                     LITPKIGGESLQRVRVFEVGASADRTSLLRLLVGAYVAGFTAIHIEARDRMPQFVRQL
                     VREFTQMTIGQEVVDETDSSIVIKDLLNPVEMPFENTIKRMHLLARGMQQDAMTAIRG
                     RDAALAHDVIARDTEVDRLHWLVARQENLIVIDAALSRRMDVTVSQAAYYFQVSRIVE
                     RIADHATQVAHNANALIDREAGGTAMVGAMDEASTLALEIFSESMGALYTGDVRKANA
                     TLQMVRDLEMKTRDIDTRALDLEAVAAIPIGQISASIRRIGEYSGDICECVINYTVGQ
                     AL"
     gene            15398..16648
                     /locus_tag="PLI76_03755"
     CDS             15398..16648
                     /locus_tag="PLI76_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOI61178.1"
                     /translation="MGTFIPIVVLVVVFLLIAVRKVGNTSLCIWQVVLSGAAAVLVAG
                     AITPADALLSINPDVMLFLFFMFVIGEALAESGYLYHLSYRLFSRAESVRHLVVLILV
                     GGGILSALLMNDTLAVVGTPLMLYFARRHGISAKLLLLSLAFAITTGSVVSPIGNPQN
                     LLIALSGGIGNPFVTFPLYLAVPTAISLLLAYIFLCRAFPGEFHRDPLVHRKEEIRDP
                     ALAALARLSLVLLVLLIAAKVAAAVLVPDIEFRLTWIAVVAAMPVLIGSSRRFELVRR
                     IDWATLVFFAGLFVLMASVWQSGFFQPLIEESSLDLAAVPVIMAMSVVVSQFVSNVPF
                     VALSLPVLAHLGTSTTGMMALAAGSTIAGNMLILGAASNVIVIQGAERGGETLTFGEF
                     ARVGVPLTVAQAAVYVLYLALFPA"
     gene            complement(16624..>17468)
                     /locus_tag="PLI76_03760"
     CDS             complement(16624..>17468)
                     /locus_tag="PLI76_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Clp1/GlmU family protein"
                     /protein_id="HOI61179.1"
                     /translation="GEGATPERVYVVGPTDSGKTTLCRYLAEQAFAHAQTAYVDCDTG
                     QSRIGPPTTEGMALSSGPTYIRFVGSTSPGGHFIQTLTAAKRLVEKAAGARVTVIDSP
                     GLVAGRVGAEFQFHMIDLLRPTRIVALQRGRELERLLANFARSPKTAVHRLPVSPAAV
                     ARTATGRRRYREERFRDYFSGSRVQEVPLRGLGLQGRLPDMANPRGIEGRLVSFNDPE
                     NFALALGIVEEISPGERTLRALAPPFDHAAVASVRFGSLALDLDAGPGLMESLHPYAG
                     KSAR"
BASE COUNT         3202 a         5407 c         5506 g         3353 t
ORIGIN      
        1 tccggatcat gaagctcgac gagggcgaca gggtcatcgg gttcacgatc ctggacgccg
       61 aggacgaaaa gtaagccccc catccttttt ttcgttcacg ctgttttcgc atccgcgccg
      121 gatactctgc ggtcgctctc cgatgcccag gaatgtcttc acctcgcagg ttcgtgcaga
      181 ttccgatcgg ctgtgctgcc cagggtgtcg gcgctacata ggaagatcgc ttcaccatat
      241 gagaaattat ttaatctaaa agagaaatac ttgttacata gtgaacgtat gaaacagcaa
      301 acgtatcgta tctgtatcgt cctggtggct gtcggcctat tttttgctct cggactcagt
      361 ctcgtgttga catccgcaaa catgatcgtc ccggccgtcg ccgtcgctgt cagcatcgcc
      421 atcctcgccc tctgccgccg caacgtgacc gaggttattg aagatgaact ctctacgatc
      481 ctgcacggaa aggcggcgct cagcgcattc gaggtaacca ttctcatcgc cgcgattggg
      541 ttcgccgtgc tgatgatctt ctcgttcacc ggagggtccg gttcgagttt gagcagatgc
      601 gaaaacgggt ctatcctggt cgtctatggc gtgctttccg cccctccgaa cccggagttt
      661 ctctacgaga atacctacct catccccgac ccggcgaacc tgaccatcga cgacgtcgtc
      721 gccctcgacg atctctttgc cgaaggccac cgtatccggg acaccacccg cgcgttcggc
      781 gcggcgctcg gggcgatcac ggtgctgctg acagggctgt acggcgcgtt tctatggcat
      841 tacaacagaa aatacggggc gtagaggcga tgaagaacaa gatcaaagtc tttcgcgcga
      901 tgcacgacat gacccaggag gaacttgccg acaggatccg ggtcacccgg cggacgatca
      961 actccatcga gcggggcaaa tacaacccct ccatcgaggt cgcctacaac atcgcgaaga
     1021 ccttcggcgt cgccatcgag gagatcttct gtttcgagga cgatgaggat gcggaggcca
     1081 ggcgatgatc ctctgtgacc gcctgaccaa ggtctacaac ggcatcccgg ccgtggacga
     1141 cctctgcctc gagatccccg ccggcgaggg aatgaatcaa aacatctata tcatgcaaaa
     1201 atatatttgc acctgtaaaa tgattacgac cagcaccatt cccgagctct cgaaactgct
     1261 tgcattcctc ggaaatgaac aaaggctcag gattctcaag tcgattgcag acgaggagaa
     1321 gtatgcgcgc gagatctcgg aagagctctc gatctcgcgg acgctcgtca acatctacct
     1381 caagcagttg gagaggatgg gtctcgtcac cggaaccagc agggtaacgg acgaccctcc
     1441 cctgatgagg aggtattacc gggccgttcc cttcgaactg gttgtaagcc tggatgcgat
     1501 aaaaaaaacg gaggagtaaa atatggaaca ttcaaacgcg cgcggatttc actggctgac
     1561 ctggacggtg atcggactcc tggcactgct gcttatcggt cttttggtaa caatcgttta
     1621 tcagccgaat ttaggcatcg tcgtgatgtt catggcaggg atattgcagt atgtcttcct
     1681 gctcgctttc atcgggatcg tggtctgtat cttcggaggc cgcctgaagg cctggctgga
     1741 gaagtatctc gacgccctgg tagagcagaa gagcaaagac acggatgccg gtgcgaaatt
     1801 aaccgtattg aacgagaaga ttggggaaat ggaaggcaag atcgaccgga tcgaggcggt
     1861 tctggtaaag atctccgagc aggagtaacg cctatggaac ttgggcagaa tcggaacaga
     1921 gactggatcg cctgggcggc ggtcgcgatc acggcgtggc tggtcatgag catcctcgta
     1981 gctctggcaa accccgttgt cgcacgctgg atctggccgg gattgctccc cgtcttcatt
     2041 ctcgcgtacg gctgccttgc cgtctgctat ctcgtaaaaa gggtgaaggg atggagcgag
     2101 aggtatctcg ataccctcct cgagcagcaa acgttcggaa acgaggaaga agtacggaag
     2161 atacaggcaa tggtcgaaca cgtcggggag atggagaaga aggtggaccg catcgaggcg
     2221 atgttgataa tggacggcga cagggagccg gagatcgagg tgcaagaggg cacgtctgcg
     2281 aatccgcccc tcccgaaagg gacgcacgaa agccccggga gcgggttacc ctatgatcct
     2341 ctgtgaccgc ctgaccaagg tctacaacgg catcccggcc gtggacgacc tctgcctcga
     2401 gatccccgcc ggcgaggtct tcgggctgct cggcccgaac ggcgccggca aaagcaccac
     2461 cattcttatg ctcgtcgggc tcatccggcc gacgtcgggc gcgtgctcca tcggcgggat
     2521 cgaggttgcc gcccacccga tcgaggtgaa gagaaagatc ggctacatgc ccgaagacgt
     2581 cgggttctac gccgatcgca ccgccgcaga gaacctcgac tatgcggcga ggttctacgg
     2641 gctcagcgaa gccgagcgga aacggcgggt cggcgacctc ctcgccctcg tcgggctgga
     2701 cggcgtcggg acaacggtcg gcggctactc gaaaggaatg cggcagcgtc tcggcctcgc
     2761 ccgggcgctc gtcaacgatc cggaggtcgt catcctcgac gaacccacgg cgaacctcga
     2821 cccccggggg gtgctggact accgcaggat cgtcagggac ctcgccggcc gggggacgac
     2881 cgtgctcgtc tcctcgcaca tcctctcgga ggtgagcaaa gtctgcacct ccgtcgggat
     2941 cctcgcacgc ggaaggctca tcgcgtccgg cgaccgggag gtgctctcgc gcgccgcgat
     3001 gcaggaccgg gtgacgatcg acgtcgagac cgggacgccg atgccccggt tcgcaagccc
     3061 ggagatcctc tccgcagagt tctcgcagga cgcctgctca gcccggatcg tcgcggagag
     3121 cgatatcagc actctcatcg caaagaccct gtacgccgaa ggcatcctcc tccggcggct
     3181 ggcggtcgag aaacccgaca tggaggatat cctgctctcg tactatacgg aggggaccgc
     3241 atgacgcgaa cggccttgcg catcatcgcg ggcaaagagt tctccgacca cctccggagc
     3301 aggcggtttc atctgctctt cgcgatcctg gccgtcgtcg ccgccgtcgg catgatcacc
     3361 ggggcggtcc agtacaccag agacctctcc gactacaacg cggtccagat gatcgcggaa
     3421 ggtgaagacg acccgacgct cgccggcaat cttgccgggc tgatgagacc ctccatcctc
     3481 tcggcatact cccagatggg aatgctgatg gccggtatcg gggtggtgct cgggatcgcg
     3541 atgggattcg acctggtgac ccgggagaaa gagagcaaat ccttgaaaag cctgctcgcc
     3601 tacccggtct tccgggacga agtgatcaac ggaaaggccc tcgggggcgt cggtgcaatc
     3661 gcgctcgcga tgggggccgt cctggtcgtc tccgttgccg tcatggccct gttcggcatc
     3721 gtcccggacc tcggcgagtt cgtccgcatc ctggtcttcg ggacggcgtc gttcctcctc
     3781 gtcttcacgt tctttgccct cgccctcctg atgtcgacgg tctcgaaaga cagcgggaac
     3841 gccctgctgt actccctgat cgtgatgatc gtcctctcgt cgttcgtccc gatcttcgcg
     3901 tacaccccgg tctactcggc cgtcttcggc gacccccccg cccagccggg cacggccacc
     3961 tactcctacc agcagggttc gtcggcctat atggtgacga cgacgaaagt cggggtccgg
     4021 gcatccggcg atgccataga cccggaagcc ctcgcggagt atgagcgggc gtcaagagag
     4081 tatacgaacc agagacggac gatcaccgat atcgtcacgc tcgtctcgcc gaccggcaac
     4141 taccagacga ttctcggtgc cgcatcgcaa cccgcagggg ataatacccc gggactcgga
     4201 agcctcctgg gcgcccttgc ctataacctc atcgccctgc tcgcgatgcc cgcggccttc
     4261 ttcgggctgg catgggtccg gttcatgcgg gaggatatcc ggtgaccgga tccgattcat
     4321 ggccgggaaa actctttttt gccggataaa cggcgacggc ggtctcgatt atcggcggaa
     4381 gagagttcgc cgttgcatcg agatgaacgg cgaacggctg ttctcccggg gatgtcggcg
     4441 gtgcgggcct gcaggtgctt atgtcccgca ggggttcgcc gaacatcctc cggaaggcca
     4501 gaactccggt tctctgaagc acctgagcga tgcggggttg tgccgctcaa gctccctgaa
     4561 gaaatagcct gcaggctcgg gaccggagtc gtatatcgcc tgctgcaggg cgtgcatcag
     4621 ccacccgcag atcgcctggc cggcatcggg cgcattcatc tcgacgaccg cctcatccct
     4681 cctgtcgttc cagcggtaaa ccatatcaac cgggaaggag agccccgcct caccggtaaa
     4741 gcgtttgcgg gcatcccgcc cggccccctg cattgtgccg gccccggtcg gaacaggagg
     4801 tttatccggg tgcagggatc atcaggaagt atcatgttcc atacaacgat cgagctggag
     4861 acgcatgggg agggagagat caccgacctc acgtttcagg tacagcaggc gatcaccgag
     4921 agcgccgtcc ggatggggct tgtaaccgtc ttcgtcacag gctcgaccgc cgccgtcacc
     4981 accatcgagt acgagccggg cgtgctctcc gacctttccc gggcgcttgc ggtcatcgcc
     5041 ccggacgata tcccctatgc gcacgatgcg gcatgggggg acggcaacgg ccggtcccat
     5101 gtcagggcgg ctatcgtcgg gccgtcgctc tccatcccgg tcatcggggg caggctcgcg
     5161 tgcggcgcct ggcagcagat cgtcctcctc gagctcgatg tcagggcaag ccgcaggaga
     5221 acggtccacg tcaccgtcca cgactgagcg ccggaaaaag gagagatgcg cgacccgggg
     5281 tgcgtgaaga cacacggcgg atagagtgat acggctgcgt ttgcaaccac aaatagtatc
     5341 gctctatcct ggtcctgccg gagttgtttt cgagaagaat gagcagttta atcgagaggt
     5401 accgtctcca gttgcgatac cagttcccag atgcgggtcc gtgcgtggac cggcatgttc
     5461 ggatcgttgc tgatctcgtc gatcatcgag atcgcggttg cggcgcggag gccgatgctc
     5521 cgggaattat ccaggaggac cgttttcgtc tcatctgcga cccgccggat attacgtgga
     5581 atagtggaat cctcgctgat gttctgcaac atctggatgc agattcgtat tgtttcatct
     5641 ggacttgcca tgagagctac ccctctatta ttaatggcat ccagacatat ataggttgat
     5701 taagggagtt tgatggaatg acggcggata aggctgacga agttatggcc gagtatctcc
     5761 tgaaaggagg aaagatgctt gcaaaatcct gtaaggtctg cgggtacccc ctctttgagt
     5821 accggggaga gacccggtgc gtcgtctgtc cgctcgcgga accggagagt cctcttcccg
     5881 tacccgaacc ggcgcagtcg ccgtcacccg cccgggaacc accgggtccg gtcgccgcgg
     5941 agatcgagcg gacgatcgtc catctctgcg agcgcatcag gagcgaaccg cgggccggcg
     6001 agtgcctgac cctgatgaag gcggtagacc ggggagcgcg ggcgctcgga aggctgaatc
     6061 agcggtaagg ccggcgggca agctcccata tcccgcgggc ggtcttttct ccgacaccgt
     6121 gcaccgatgc aaggtcctcc gcatcggcat cgaatatcgc cttgagcgac ccgaagtgct
     6181 caaggaggag ccgggcgctc ttcaggccga tgttcgggaa cgccgcgagc gcatactcct
     6241 gctcctcgcg aaccgagcgg tgggacttct tcgggtgcac cttgcgctct cctcgctcgc
     6301 ccccctcccg ctgtgcgagg acatagagca tcccggcggt gtcgtccgca tcccgcgtgc
     6361 ggatcagcgt gacccccata ccgaccgtta tggcggcaag cgtgcccctg acggcgttcg
     6421 ggtggaggtt tcgcacaccg tacagatcgt ctcccccctc gacgatcagg accggccgcg
     6481 gcaccgcgtc ggccatggct cctacctgcc cgaagaggtc ccgctcgatg agggtgtcca
     6541 cgaagtcctg cacggtcttt ctctcgacga ggatacggtc gcctatggcg tagtcgccaa
     6601 ggtcgaggcg ctcgatcgtt atcgacgccc cgagctcgtg caggcgctcg atgacccgcg
     6661 acgacatctc gcggtcgtcg acgacgatcg ccggcccgga ggaggcgaac gcgaggatat
     6721 cggcctgccg ggtggcggcc ggtatcgacg gcgcgggggc gggaaccggg gtgagagtag
     6781 aaatggagtc gggaaccgga acaggtgcaa cgctcatatc cctgatcccc tttaccatcg
     6841 cccgctcccg ggtctggctc acgtagcgga acgtctcgtc ggacgtgccc ttcgtcacca
     6901 ggacgacgat cgtgccgctg ccgctcctcc cggtcctccc tttcctctgg atgctccgga
     6961 tctccgaggg cacggcctca taaaagatca ccatgtccgt cgaggggaca tcgagcccct
     7021 cctcgcccac cgatgtcgcg accaggcacc tgaactcgcc ctcgcggaac cgggagaggc
     7081 tcgcgatctg ctctttctgc gagagccccc gctccgcgtc ccgcgacgcc tgcccgacga
     7141 accgctcgca ggcgaccccg ctcgccgata tcgcgtcgac gagcgtctgg acggtgtcgc
     7201 ggtaggtggc aaagacgatg atccggctgt ccgggtgcgc cgcaagctgc gcccgcacga
     7261 tctcgcagac gatcgggact ttggggtgga gctcttcctt ccagccgccg gcgacctcga
     7321 caaggcgctg gtaggccgga tcgccgatga ggtgcttgct tgccctgctc ccggcgcccg
     7381 aagctccctc cgcgccgagc cgggcgagat acagtttgag cgcctcgctt ccttgcgtct
     7441 cggcaagcga tatggcgtgg cggagtttca tgcactcggc gtgcagcgac gccgcggcga
     7501 aggcggcagg gtcccgcgcc tgtatgcgct gctggatctg cgcgttgaga aggttcaacg
     7561 ccttgatcga gagtttgtcc ggcttcggga cctggaagcc gaggctcgca agacgggtga
     7621 gacgcgcctc gacgagcccc cggagggcga cgagtgccgc ctgcagttct tcgggaaggt
     7681 agacgtcgac gtactggatg tcccgctcgt ggatgtaggg ccggacatcc tcgtcggtct
     7741 ccacccgggt ctcgaccgcc tcgacatgga ggtttgcaca gacctcctgc accttcatcc
     7801 gatcccctcc cggggatgcg gtcatagcga gcagcagggg cttctgtgcc gtcgtgcagt
     7861 agtgccgggc aaggaagacg taggcgtagt ttcccaccgc ccggtggcac tcgtccacga
     7921 ccagcaggac gacgtcggcg aggctgtacc tgccggccag gcagtcgttc ttgatcacct
     7981 gcggcgtggc aaaacagatc cggcacgcct cccaggcttt cgcccgatcg tcgggggacg
     8041 tatccccggt gaacatggca aagtcggact cttccggctc ggaagcctcc cggaagagga
     8101 gaaaccgttt gaaaaaacgg aggtgctgct cgaccagggg ttttgtgggt gcaagcacga
     8161 gcacctttcc cgggtgggag tagagacggg atgccgcgac gaggagcgcg acggccgtct
     8221 tcccgaggcc cgtcgggagg acgaccatcg tgttcgcgtc aagcgcccgg agggcgatgg
     8281 cgagctggta tcgccgctct tcgatgctct ccggccggat cagcgggtgg gagacagagg
     8341 tcataccctg agttcgcgga acgcggcggc gccggagatg agcgcggaga ggacctccgg
     8401 cagccgttcg accggtacgc ggacctgctc catgctgtcg cggtccctga cggtcacggt
     8461 gttgtcctcg agcgtatcgt agtcgacggt gacggcaaaa ggcgtcccga tctcgtcctg
     8521 tctccggtag cgcctgccga tggcgccgga gtcgtcgtac tgcgcgagga tgcggcaccg
     8581 ggtgagccga tccgtgatcg cctgcgcgat accgtcgagg ccgtcacggt tcatcagcgg
     8641 gaagacggcg gcctggaccg gcgccaccgt cggcgggagg cggagcacct ttctcacctc
     8701 gccgtccacc tcgtcctccg cgtaactgtg ctcgagggcg acgtagatca tgcggtcgat
     8761 gccgtacgac ggttcgatga cgtggggcat cacctcctcg ccgcggacct cgacctcctc
     8821 ctcgcggacc tggtagaggt cggcgggtat gaagaactct tcgccgtcga cggtgacgcg
     8881 ggcaccgtcc tccccggggt ctgaggcggc gagagcatcc gcgatcgcct ttgccttccc
     8941 gcggaaccgg gggccgagga cgcccatatc ggcaacgatc cgccgtttca cgaccctcct
     9001 cggttcgtcg tagggtacga agaccgtcat cgacgtgccg gaatgcgcgg cgtgcgagcg
     9061 caggtcgtag ttggtgcggt cggcgatccc gacgatctcc acccacccga accggccgga
     9121 gtaaacctcg gcgtcccagc agtcggccgc atagtgcgcc cgttcgtccg tcaggtgctg
     9181 gcggaaccgg agccgcgatg ggtccacgcc gatggctagc aggagctcgt gcgtgagcgc
     9241 tatgtagtag gcgacgtact cgtttgctat cacgccctcg tccaccgcgg cgcgcatcgt
     9301 gattgcggcc ggctcgaggt tctcgacctg ccgcgcgatg gtgaggagag gcgccgtatg
     9361 gtctgcgtaa cgatagaacg cggggtgatc cttctcatcg ggatggacga agatctcggc
     9421 ctccgcctgc gagaactccc gcaaccggat catgccctgg cggggggata tctcgtttcg
     9481 gtacgacttc ccgatctgga cggcgccgaa ggggagccga tcacggtaga aacgcaggag
     9541 gcgggggaaa tcggtgaata taccctgggc ggtctcgggg cgcaggtagc ctctccgctg
     9601 cgacccgggg cctatggtcg tctcgaacat caggttgaac gcaaagacgc ccgcctcccc
     9661 caggcactcc ttgcacgccg ggcaggggag ctcgtagagc gccgcctgga gagccgcggc
     9721 cgagagcgtg ccggcgttct cgatgccgtt ctccgccgcg atgtgatcgg cgcggaggta
     9781 ctcgccgcag tgcggacact gcaccatctt gtcggcgaac tctttcacat gaccggatgc
     9841 gacgaagatt gcttcggttc cgacggtggg gcattcgatc tcgtagtagc cttcctggaa
     9901 gacatagaac gatcgccaga gattctcgat gttccgcttc agcatcgctc cgagcgggcc
     9961 gtaatcgata aaaccggcga ccgacccgta tatctcggac gagggccaga caaacccgcg
    10021 tcttctggct acatccatga ctttttcgta aatatcgctc gtctcggcca taatcctgca
    10081 gtacacttga gcgccggtgc tattaaagaa tggttcttcc cctgcccggg atcaaaattc
    10141 ttcgaaaagg ggctgtacga ggtggaattt ttttcaccac cggcaatata ccaaaagata
    10201 ttgttatatc ccggaaaaag atccgcttcc cttcacggat tatatagaaa gttttaaatg
    10261 gtagcagttg taatgtaggg agcgttacgc cccctcgccg ggggataatc tatatatgtc
    10321 cggggtcgtc atgaatgacc tccagagtcg gatgatggaa tacaggacag agaggcacgg
    10381 atatggcaga agatacccgc aagcagcagc tccttgagct gtttacgacc atcacgaaca
    10441 aaaagacgat cgttgaaccg atgaggaagg ttcacggtac gctccgggac cgcgacgctg
    10501 tcgagcgcga gatcgccctg atcatgaggg aagtcctcga tcaggggtat ttcaagacca
    10561 aactcgctcc acgacagctc gcaagactcg tggtcgcgta ctacgacggt aagaatgata
    10621 cggagatcgc gagggcgctc ggcgatgaga aactgagcaa gaccgtggcg cgtgcccggg
    10681 tccgcctcaa gctcttccgc gacctcgact tcaagatgcc gttcgatcgc ggaatgatga
    10741 cggatctcct cgattcgggg aagactatga aggagatcag cgaggaactc gacgtgagcc
    10801 cctcgacgct ccgtgaatat cgccatgtga tcgaacagga gagggatacc acgctcgacc
    10861 cgtacctgga acggatcaga gacgtgatgg aagaccgtga cctctccgaa cagatgacgc
    10921 gcggtgtcgc aaacgacggc ctctccgagg cgatcgatat cgccgaggcg atcgatatgg
    10981 gggagttctg aatctatccg tactcacccc ctgcctttct cccaacattt tttaagagat
    11041 agaccgatct cttctccatg aggttgacct ggctcggcca cgcctgtttc ctgctctccg
    11101 gatcgaagac gatccttatc gaccctttca tcccggaggg tacactccct gtagagcccg
    11161 atatcgtcgc cgtaacgcac gcccacgcag accacctcgg tatgacggtg cagttaggga
    11221 agaagacggt cgccgtcaac gaggtggcga agtacctggc agcaaagggt ctcccggtag
    11281 aaccgatgaa catcgggggc agcatcaccg tcgacggcgt ccggtttacg atgacgcccg
    11341 cgctccactc atcgtggctg gagaatgagg gcatgggttg ttacggcggc gttgccggag
    11401 ggttcgtcgt cacgatggac ggcacccgca tctaccatgc cggcgatacg gggctttttt
    11461 cggatatgca gctcatccgg gacctttact ggccggacgt ggcgctcctc cccgtcggcg
    11521 ggcgtttcac gatgggaccc gatgaggcga tgatcgcggc gcggtacatc ggcgcgccgc
    11581 tcgtgatccc gatgcactac gacacgtttc cggccatccg gcaggatctg gaggggttca
    11641 aacgggcgat cgaacggacg acgtcgatcc gggtcgcggt catcacgccg ggcgagagca
    11701 tcgaggtcgg cagagaaggg gcgtgacggg cgccctcatc cggcgcggcg gatcctcgcc
    11761 ggtaactgga gggataccag aagaggtttg cggtaagcgc ggtttgagag aaaaggatac
    11821 acgaaaagac gacgtcaagg ggggaagtgt cttgtgtgga tccagatctc tctctgggta
    11881 attgggcggt actactatat agttatttct aactgaaaag gctgtaaaat aaagattccg
    11941 ggtcgttgcc cggagcctgc acgggttacc gcgcaggagc cgcctccgtt ctctcttctc
    12001 gtgcccgggg tcccgttaca acgggattat ccggatacct ggtccattcg gtgattgacc
    12061 cttcatagag ccggacgttc gggtagcggt agagccattt caggaggacg aactcgttcg
    12121 tggcttccct gccggtcccg caggtgcaga tgacggtcct gaaccggtcg acgccgtgcc
    12181 cttcaaggat cgtgtcgagt tcgtcgttgg acttgaacag cgccgggttc gcgtcgtcca
    12241 tcaggcttcg ccagggcagg ctgatggccc cggggatgtg cccgcgcgcc cgccaggggc
    12301 cgctcgtccc ctcgtaaaac gccttcggcc gcgcatcgag cacgatcacg tcgtcgttat
    12361 ccttgatctt ttggaactcg tcgtacccga cggcatagtc gttacggacg tttacggtga
    12421 acccggacgg ctcgacggac gggaactcct gcgagagttc tttgccctcg ctcttccacc
    12481 cgtctatccc cccgtcgagt atgtacacgg tgtcgtgacc gtactttgca agcgtatagg
    12541 ccatcatcac ctgtgcgagc ccgtctcccc agccggagaa cacgcccttc ccggtgtaga
    12601 cgacggccgg cgagtcggcc ttgaggcccg cgcgccggaa cgactcctgg atgcaggcat
    12661 ccgggctgta cgacgtcgga aagccgcggt tggatacccg cagaacccct tcggtcaggt
    12721 agacggcgcc ggggatatgc tcctggatgt agtcgtgcac attcggctgg gtatcgatga
    12781 tcgtgagatc ggcatcgtcc atgtggtcgg cgagccatgc cggagtgacc agtttgacct
    12841 tcccgtcgcc acggggatac ggggcgttct cgcggtcgct tttcgcctgc agtcgttttc
    12901 tgccgccagg ggtaaacata ttctcttcca ttcccatcac ctcgtgcagg gtgtcctcac
    12961 tggagggcgg ccttcagtgc aggtctgaag ttatatacct tatgcaggac ccggcgggga
    13021 gcgggaacaa cgagtgccat catgcaccgg cgaccctctc ctcgcaggaa caggggttcc
    13081 ccgcaccggt cccgggtgcc gatcgccagg ttcttctccc cggaccgacc actggcggga
    13141 gaccagcgaa gatggcggag gatgtatcgg gggttctccg ggatcgcccg atccccggca
    13201 gaaccaccgt ccttcccggg acggactcat gaagaagatc atcgtatacg tccggaaaga
    13261 cgggcgtgat cgggtagaag aggctctcaa ggggctccgc tatacggtat cgctcgtgca
    13321 ggacatctac gagatcgtgg tgtatacgcc tgaggagaac ctcgacgatc tggtcggcag
    13381 gctccgggat gtgctggacc tgcagcatga cgagaacatg atcgaggtat ccacgccgga
    13441 cttcgtcgtc tcgtcgctcc ttctgcacgc cgagcaggaa gagggcagaa aagaggagaa
    13501 gacgccggtc gaggagttga tcgactcggt caaggggtat gcagaactcg accttgagaa
    13561 agtggcgctg acatccatcg ccggcctgat cgccctttcc ggcctcttcc tcgacaaccc
    13621 ggtcatcatc atcggggcca tgctcctctc ccccatcatc ggcccgatct acgggtttgc
    13681 catctatact gctcttggca tgatccggga gtccctgaag tgcctgggcg tgctctcggc
    13741 tctgctcctg ggcgtctttc tcctctcggt gatggcaacc ttcctgatcg ggagcgccat
    13801 gccgcttgcg ctgaccgatg agatcacctc gcggctcgtc gccaacccca tctacaccct
    13861 ggtggcggtc ctcctcgggt tcgtggcggt gctggcgttc aatcgcggca catcggaggt
    13921 gatcgccggc gtcgccatcg cttccgccat catcccgccg acggtggtgg ccggccttgt
    13981 cctggtgctc cagcccatga gcctggtgtc gtcggcgctc ctggtggccg ggcagatcgc
    14041 cgggctgatc acgggggcgc tcgtcgccgt gaccctgcaa aagatcgagc cgcgggagac
    14101 ccgggatcgg accgtcgcac gacggcacct gaggtggtcg gtcaccctga tcgtgctgct
    14161 ctttgctctg ctcctcggga tcacgtggct catgtggcgt taaggcgcct ctccggatga
    14221 tagatattcc cgaaagtata tgtatctccg ctaatattgt gcgacgtata tatattcggc
    14281 gtatgtatat atcccctcga tgctaacacc cgggcatcca tggagatcag aaaagttcag
    14341 ataaccggcg gttcgtcata catcgtatct ctgccaaaac agtggatacg gtcgacgaag
    14401 atccagaaga acgaccctgt ggggttgatc gtacagcccg acggttcgct cctgatcacc
    14461 ccgaagatcg gcggggagtc gctccagcgg gtcagggtgt ttgaggtcgg cgcctcggcc
    14521 gatcgcacct ccctgctccg ccttctcgtc ggggcatacg tcgccgggtt cacggccatc
    14581 catatcgaag caagggatag gatgccgcag ttcgtccggc agctggtcag ggagttcacg
    14641 cagatgacga tcgggcagga ggtggtcgac gagaccgact cctcgatcgt gatcaaggat
    14701 ctcctcaacc ccgtcgagat gccgtttgag aacaccatca aaaggatgca cctccttgcg
    14761 cgaggcatgc agcaggatgc gatgaccgcc atacgggggc gtgatgcagc gctggctcac
    14821 gatgtcatcg cccgggatac ggaggtggac cggctgcact ggctcgtcgc gcggcaggag
    14881 aacctcatcg tgatcgatgc cgcgctctcg cgccgtatgg acgtcacggt gagccaggcg
    14941 gcgtactact tccaggtcag ccggatagtc gaacggatag ccgaccacgc aacacaggtg
    15001 gcgcacaacg cgaacgccct gatcgaccgc gaagccggtg gaacggcgat ggtcggtgcc
    15061 atggatgagg caagcacgct tgcgcttgag atcttctcgg agagtatggg agcgctctac
    15121 acaggggacg tcaggaaggc gaacgcaacc ctccagatgg tgcgcgacct tgagatgaag
    15181 acccgggata tcgatacccg ggccctcgac ctcgaggcgg ttgcggcgat acccatagga
    15241 cagatatcag cgagcatccg gaggatcggg gaatattccg gggatatctg cgagtgcgtc
    15301 atcaactaca ccgtcgggca ggccctctga gagccggggc agcacccttt tttgggccgg
    15361 cggccgacgt tcgatcagca tatttgccgg tggtagcgtg ggcaccttca tcccgatcgt
    15421 cgtccttgtc gtcgtctttc tcctgatagc cgtccggaag gtcgggaaca cgagcctctg
    15481 catctggcag gtggtgctct ctggagcggc tgcggtcctt gtcgcgggtg cgatcacgcc
    15541 tgcggatgca cttctgtcca tcaaccccga cgtgatgctc ttcctcttct tcatgttcgt
    15601 gatcggggag gcgctcgcag agagcggcta cctctaccac ctctcgtacc gcctcttctc
    15661 ccgggcggag tcggtccggc acctggtcgt cctcatcctc gtcggcggcg gcatcctctc
    15721 ggcgctcctg atgaacgata cgctcgcggt cgtcggtacg ccgctcatgc tctacttcgc
    15781 ccggcggcac ggcatatccg caaaactcct gctgctctcg ctcgcgttcg ccataacgac
    15841 ggggagcgtc gtaagcccca tcgggaaccc gcagaacctg ctcatcgcgc tctcgggcgg
    15901 catcggaaac ccgttcgtca cgttccccct ctaccttgcg gtcccgacgg cgatatcgct
    15961 cctgctcgcc tacatcttcc tttgccgggc atttccgggc gagtttcaca gagacccgct
    16021 ggtccaccgg aaggaagaga tccgcgaccc cgccctcgcg gcgctcgccc gcctctcgct
    16081 cgtcctgctc gtcctcctga tcgccgccaa ggtcgccgcg gccgtgctcg tcccggatat
    16141 cgagttccgg ctgacctgga tcgcggtcgt tgcggccatg cccgtcctca tcgggagttc
    16201 gcggcgcttt gagctcgtcc gccgtatcga ctgggcgaca ctggtcttct tcgccggcct
    16261 cttcgtcctc atggcgagcg tctggcagtc ggggttcttc cagccgctca tcgaggagag
    16321 ttcgctcgac cttgcggcgg tcccggtcat catggcgatg agcgtcgtcg tctctcagtt
    16381 cgtctcgaac gtccccttcg tcgccctctc cctcccggtc cttgcgcacc tcgggacttc
    16441 cacgacgggc atgatggcgc ttgcggccgg gagcaccatc gccgggaaca tgctgatcct
    16501 cggcgcggca agcaacgtca tcgtcatcca gggcgcggag aggggcggcg agacgctgac
    16561 gttcggcgag ttcgcccggg tcggcgtccc gctcaccgtc gctcaggcgg cggtctacgt
    16621 gctctatctc gcgctcttcc cggcataggg gtggagcgac tccatcagcc ccggccccgc
    16681 atcgaggtcg agagcgagcg agccgaacct gaccgacgcg acggcggcgt ggtcgaaggg
    16741 cggtgcgagg gcgcgaaggg tccgctctcc gggggagatc tcctcgacga tgccgagggc
    16801 gagggcgaag ttctccgggt cgttgaagga gacgagcctc ccctcgatcc cgcgggggtt
    16861 tgccatgtcc ggcagtcgcc cctggagccc gagcccccgg agcggaacct cctgcacccg
    16921 gctgccggaa aaatagtccc gaaatcgctc ttcacggtag cgccgccgcc cggttgcggt
    16981 ccgggcgacg gccgccggcg ataccgggag ccggtggacg gcggtctttg gggaccgggc
    17041 gaagtttgcg agcagccgtt ccagttcccg cccgcgctgg agggcgacga tccgggtggg
    17101 gcggagcagg tcgatcatat ggaactggaa ctcggcgccg accctgccgg cgacgaggcc
    17161 gggcgagtcg atgacggtga cgcgagcccc ggcggccttc tcgacgaggc gttttgccgc
    17221 cgtcagcgtc tggatgaagt ggccgccggg ggaggtcgac ccgacgaacc ggatgtaggt
    17281 cgggccggag gagagggcca tcccctcggt cgtcggcggg ccgatccggg actgcccggt
    17341 gtcgcagtcg acgtatgcgg tctgcgcgtg ggcgaaagcc tgctcggcga ggtagcggca
    17401 gagcgtcgtc ttcccgctgt ccgtcggtcc gacgacgtat acgcgctcag gggtcgctcc
    17461 ctccccga
//
LOCUS       DAOSEE010000030        17348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1337_length_17348_cov_241.160354, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000030 DAOSEE010000000
VERSION     DAOSEE010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 17348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17348
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1337_length_17348_cov_241.160354"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..1208
                     /locus_tag="PLI76_03765"
     CDS             30..1208
                     /locus_tag="PLI76_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HOI61180.1"
                     /translation="MKVCIMCGGEGTRLRPLTFGRPKPCIPIVNKPSIQHLVSHLANL
                     GFNDVIITLGYMSDAIEKALGDGSLFGVNVTYVHEKTKLGTAGSVKNAQRYLEEQPFL
                     VVGGDHVVSLNLLEFYREHLANDSLTTIGLISIDDPTEYGIAEIDANYQIKRFKEKPG
                     PGEIFSNLASTGMYVCDPEIFDHIPTGEKFDFARNLFPKLMEEGHILKAWLARGNWSD
                     VGSPRSLREAERWKLQEIGFTNISGDLYIKGARIMGPAQIGSCVSVAANSRVIGPVSI
                     GAGTIIEENVVIGPYTSIGEGCIIQNSAKIFSSSIYNRVVVGRDSTVSGSIIDNDTLI
                     GSGCNIEHDTVIGPHVVLKGGAIVHSGTRIWPKVIIPEGTVVKEHFLNEEYDTRTEGS
                     "
     gene            complement(1205..1993)
                     /locus_tag="PLI76_03770"
     CDS             complement(1205..1993)
                     /locus_tag="PLI76_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOI61181.1"
                     /translation="MDKKRVKDYMTYDVVTVDAHGTVRDVIETIKRTRHDGFPVVENS
                     KEVVGYISARDLLFAHPATPVEQVMSRHLIVADPDMSINDAARVIFRSGIQKLPVVDE
                     KNELIGIISNADVIRSQIEHVSPDKVFKFIETLRKLYGVEPTLRRGPVPINDLLPTQS
                     KIYEDELEGRMYEIKKGLAEPLIVVRRPGRWILVDGHHRAIAAKRLGIKNLDAYIIEV
                     RENIELGMERTAKTLNLNTLDDIKVLDYARHPLVALTHRLVRHG"
     gene            complement(2058..2528)
                     /locus_tag="PLI76_03775"
     CDS             complement(2058..2528)
                     /locus_tag="PLI76_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOI61182.1"
                     /translation="MARIDRGPYRTIWQDFDDLMAEMENRFQSMLGGIGARGEEIRGR
                     VVPAVRDFRVDVRDHEDEVIVVADLPGVEKEDVAVRITDPLHLEIASTRTSETGEEGQ
                     DFFMRERIYGQMSRMVLLPAEVTEENAAASFKNGVLEVRLKKVPAEIGTTIPID"
     gene            3049..4338
                     /locus_tag="PLI76_03780"
     CDS             3049..4338
                     /locus_tag="PLI76_03780"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI61183.1"
                     /translation="MKISEGDRVVFALSFARMGDAIGNSILIIVIPLLTAQLPAPLFP
                     IPGPARVGVLIAIYGLLAALVQPISGVLIDRTNRHKLFILVGLAMVAIATFGYSFATL
                     FESLLGLRILQGIGFALTIPASVAIVTIVTQKMTRGGSMGIYNSSRFLGFAIGALIGG
                     YLYTTYGPNMAFYVGTGFIILAIVLVQAFVKEVEEPEEPRDDPEVPAGPIHQEENAAF
                     EGGKRTLPGLRGLGFISAGIGGLAFAVFLMASAFAMLVTLEPQFNIRLHETAFAFSIA
                     FSTLAATQVVFQYPIGRVSDRWGRKPFVIAGLLLIAPTTAALGFISTTFQLVSLRFIQ
                     GIATAAIAAPAFALAGDLSKRGTQGRQLSVVTTGFGLGIALGPLIAGFLVVYGFEVPF
                     LVGAALSLLGAWVVYRYVPETVEREPGARSFRWPGRR"
     gene            4417..5295
                     /gene="cyoE"
                     /locus_tag="PLI76_03785"
     CDS             4417..5295
                     /gene="cyoE"
                     /locus_tag="PLI76_03785"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HOI61184.1"
                     /translation="MEHRGVLPAYLELTKPKVVFLNLFVGLVSFLLAASHPPPVHLCT
                     FLAAGYLVAGGCGAINCWYDRDIDRVMARTSGRVIPRNALSPRRVLLFGSLLLTTGLL
                     AAVLILPFLAAAMMAAGAGIYLIIYTAWLKRASHLNVVLGGTAVCFAAFAGWTATGAP
                     FPTLTPVLIALVGFLWTPGHFWALAIYRDQDYRIARIPMLPARAGRYPAAQAIFLWNA
                     ATAGLTVLIPFLTAIDRITLAITIPAGLFLLSLSRRLLVSPTDERAVRLFSFSIVYLM
                     SVLFAFLVLADGLPAL"
     gene            complement(5332..6078)
                     /locus_tag="PLI76_03790"
     CDS             complement(5332..6078)
                     /locus_tag="PLI76_03790"
                     /inference="COORDINATES: protein motif:HMM:NF021214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HOI61185.1"
                     /translation="MEGNGKADRGRTGRSTKIFASFTAVATLIAMAYLNPALGESFTI
                     HMVYHMILIGVLAPSLLAADFFLWWLPPGTLRKRTLYRTLRRGLYAISYPVTAFILST
                     AVLWFWHIPIPYDVTLTDMPVHILEHVTLLIAFIAYWAPLVPGSRLHLPVIRTNEGKA
                     LYLLAGAMQGMILGAIITFQDQIVYLYPSTAHLPGVTLLGDQEMGGAAMWFIGAIIYA
                     VAAILSFRSTDPDIHRDVPPARGAIGGQEE"
     gene            complement(6082..7593)
                     /locus_tag="PLI76_03795"
     CDS             complement(6082..7593)
                     /locus_tag="PLI76_03795"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HOI61186.1"
                     /translation="MSSDAVPPVKTPSLPANYGGVLRFTLTGMVFAFVMFVIMGIFGL
                     TMRTEQSGLLNLLGPELFYRIMTLHGLSMVSLALLGSIAGFAAVIGRRVVLDTRWLWA
                     TFFFFFIGMMFVLFSTLGGGFAAAWTALYPLPFHGGYAFWGVIGATVGIGFVLVGLLF
                     YCVLITRTVSRAHGGVINALGWPLIVRRTARQENLSILDVLAVAVAIPGIVAVIAGFT
                     WLIPLWLQASGLIQSIDVLFMKNFDYLFGHLLVNLTIYFAAGLMYFLLPAYTGRAWKL
                     SRTYALALNAITFIVPIIYLHHLYQDFAQPLAFQVAGQIGTYVSVIPVILITLFGGLS
                     QYYRSGIRWDVTTIFLAIGLWGWMFGGMAAALDATIAVNQVMHNTLWIPGHFHTYFLL
                     GAVVFLWGFLFSILRTLSGAGSSPRTRYAAAAYGVGGAGFTLMFLASGASSIPRRYAV
                     HFPEWQIFASIAVPFIILIGVGVIWIGYVMLSRVPRAWERTRGPVDILPEGGS"
     gene            complement(7583..8116)
                     /locus_tag="PLI76_03800"
     CDS             complement(7583..8116)
                     /locus_tag="PLI76_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61187.1"
                     /translation="MIFIDPRYVFVLLLVMGGSYTLLWAWVMRSSREKVGYDDITEGL
                     ELLRRRLYSVIVLAAVVVFLGTQVALPYQPVRDITVGEPVLVINATTSQFAFTLSNTT
                     IPANVPVEFAVTSIDVNHGFGIFDPDGRIIAQTQSMPGYTNRLIVNFTRPGPYRIHCL
                     EYCGVDHWGMEAAFNVE"
     gene            8384..8821
                     /locus_tag="PLI76_03805"
     CDS             8384..8821
                     /locus_tag="PLI76_03805"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOI61188.1"
                     /translation="MAPVTDGDTLLLHFTSTRPDGEIFEDTRAGEPVRATLGERQINP
                     LFEKALIGREPGETVTVVLPPEKAYGKYRSRLVITMKRKRLNLDHEPVPGEFLKVEVM
                     GKPCLVTVTDVTEKSVTIDANHPLAGETITYAITVAAILPRSG"
     gene            8989..9657
                     /locus_tag="PLI76_03810"
     CDS             8989..9657
                     /locus_tag="PLI76_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOI61189.1"
                     /translation="MKIVGINGSPRGGRSATLQLIEAVLEGAKEKGAEVDVVHLIDLD
                     LKFRNACSACFQEGKCVDEDDFPNLYEKIKAADGLVLGSPVYFDLVTGQMKLLIDRMA
                     DGIQCQSLTGKYGCSVATSGDHAEPAVVAYLNHFLQMLGATPVGNVGVAIGKDPGALA
                     KAKETAHELGKTLAEAIQTRRQYPQIEAFHKRFQEKFKAVIAGEKPEWPGDYDHWVNQ
                     AWVW"
     gene            9869..10183
                     /locus_tag="PLI76_03815"
     CDS             9869..10183
                     /locus_tag="PLI76_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOI61190.1"
                     /translation="MPEFANPFSGNRMERRLDRGELIRTIRFSIAAEYEAVQFYEQIA
                     ESTDDPLVQKVMRDVANEEKEHAGEFLRLLREIEPTEDGFYQHGYKEVEEMIAEVKKG
                     GR"
     gene            10485..10886
                     /locus_tag="PLI76_03820"
     CDS             10485..10886
                     /locus_tag="PLI76_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOI61191.1"
                     /translation="MKKGFVTDIETETVKNTDFRRVLYTGKFSQLVLMCLKPGEEIGA
                     EVHDDVDQFFRFEEGEGAVVIDGVKHAVRDGSAVVVPSGANHNVLNTSKTADLKLYTI
                     YSPPEHQDKVVRKTREEAMAREEHFDGKTTE"
     gene            complement(11243..11665)
                     /locus_tag="PLI76_03825"
     CDS             complement(11243..11665)
                     /locus_tag="PLI76_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOI61192.1"
                     /translation="MDLAMIEDLERQGLIQRLPPDPKKVADAMALARRDIDTARTLLS
                     TDCDWAYTIAYNAALQAGRALMFAKGYRPDGANQHISVVKFAGLFLSEGDALVFDRMR
                     RKRHGSVYDTVGTISAAEAEFAVSAAVALVQKVTTLLG"
     gene            complement(11637..12212)
                     /locus_tag="PLI76_03830"
     CDS             complement(11637..12212)
                     /locus_tag="PLI76_03830"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOI61193.1"
                     /translation="MLEALIPSKARVKLLTLFLLNPESEFYIREIVRMTGENINGVRR
                     ELANLESFGLLIGRRRGNQHYFTVNRDFFLYTDLQQLVLKTEGVARVIRENLSSLQNI
                     ECMFIYGSFARGTAGGRSDIDLFIVGDVNEEVLIPLVHTGERAINREINYTLMRGSEF
                     AQRRETGDPFVKNVMGERKIMIIGSCDDRGS"
     gene            complement(12407..13816)
                     /locus_tag="PLI76_03835"
     CDS             complement(12407..13816)
                     /locus_tag="PLI76_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI61194.1"
                     /translation="MIREFVDRETERALLEEEWQKDGGRLIILYGRRRIGKTRLVTEF
                     VRGKEGILHFAEDTAPQIQIRRLQEAIARSFADPLLATLDITTWDQLFTYITRIPLTE
                     RRYLVIDEFIYLIKNDPTILSALQKAWDTALAGSNWCILLSGSTLGLMTDSALSYTSP
                     LYGRRTRDMLLGPLRFRDARQFLPSFSFGDALKAYLTIGGIPEYLLKAGDYRSYETFV
                     EREFFNRYGYFYREPYFLLSQEFRELKTYQAILNAIAVGNTAPGTIAQFCGMDARHLY
                     PYLEAMTRLRIIERELPLLASRRQGLYRIEDCIFDFWYNFVFSHRQAIEMENHPIDTI
                     DTNPYFGKQFEVFMRNELARAAFPGYTIGRWWYRGDEVDLVATRESDASIVFGECKWG
                     DLTEREARGVLTRLVQKAERIRHDRYTHERFCLVAGGVEGKGRLREEGFLVYDLADVE
                     AACSAGQAPARTYSSPEER"
     gene            13979..15178
                     /locus_tag="PLI76_03840"
     CDS             13979..15178
                     /locus_tag="PLI76_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI61195.1"
                     /translation="MSSPPQRMIRRDAEAQIRSLALGFPAVAVIGPRQSGKTTLVRSV
                     FPHLPYVLLEDPDTRAFAEEDPRSFLIHYEKTGAILDEVQRVPELFSYLQGVLDANQR
                     PGQFILTGSQNFLMMERISQSLAGRVGIVKLLPLSMGELARAGLEAERYEDLLYAGLF
                     PRPYSSAVHPRDFYSSYIQTYIERDLRLLKQVQNLSAFQTFMKMCAYRSGQVVNYSSL
                     AHDCGITHNTAKEWLSLLETSMLIVQVRPHRKNFNKRLVRMPKLYFTDPGLAAHLAGV
                     QGADDLIYHPLRGGLFESLIIAEFLKFRLNRGKESNLYFWRDKLGHEIDCIIEYMGWD
                     PIPVEIKSGRTPSPDFFNEIAYWNELSGNAPDRSFVVYGGDRSQRRAAGHLVGYRDLE
                     PIIRYLE"
     gene            complement(15288..15998)
                     /locus_tag="PLI76_03845"
     CDS             complement(15288..15998)
                     /locus_tag="PLI76_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61196.1"
                     /translation="MEEQVGRLLDDLDFPALYRGYTWRDDTWEKGFLEILDLEREVTA
                     AARSLALGLDHVQKIARRDDLSAPARIAVTLYIGSAPAYWLMLEPEETIQTVERQIRG
                     LGPVCASKLLRFAVPQVFGTLDARLIRVFGRGDSGSQRYPLLDLAVEPSGDRWAIPAG
                     QPGWPGEYGAWAGALQAVAGGLNRDEVSCPHPAGFAAAGLRSEGIWAAADVEMALSCY
                     AAGVLRGEERKDSVTGVV"
     gene            complement(15998..16345)
                     /locus_tag="PLI76_03850"
     CDS             complement(15998..16345)
                     /locus_tag="PLI76_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61197.1"
                     /translation="MRTNLQGLAGRRGTAVFRQYGACRGRGCAGRSILLGQVRDLADR
                     ARINYTAGFDAVGDLRRGDIVRLTAAIDGYVKGYRREKIDDRLMRPPAVDYRLKFPRR
                     AERIGGAPSPEVR"
     gene            16423..17265
                     /locus_tag="PLI76_03855"
     CDS             16423..17265
                     /locus_tag="PLI76_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6293 family protein"
                     /protein_id="HOI61198.1"
                     /translation="MSMTTKNDPLPGGLRDVVHIIPLGHEIDRAVAPFTKNKADRAYI
                     LAVPPDAGLDPRMVRKQHYFVGRVTEQLQDLGIAVTFVPVAMFDILDVLKVVSYLIRT
                     EKEAGNAVYVNMSSCGRKTSVAVTLAAMVHEAMLYYVSADRYATADGEECEHGLSIVK
                     TVRTEVFQPFRIMMPRPENVRLLVELFRRKEEGGMTSEEIIEFFHDANTHGYERLHTA
                     ERDGYQRAKKKRALLNRTNRGYLKELEEQGYVEREWRGRHFSIRITKAGEHIACVSGL
                     VPGG"
BASE COUNT         3352 a         5389 c         5434 g         3173 t
ORIGIN      
        1 ttaaatcgat acgcagacaa ccgtgtctca tgaaggtgtg cataatgtgc ggaggggagg
       61 gcacacggct ccggcccctc acattcgggc gtcccaaacc ctgtattccc atcgtcaaca
      121 aaccctctat ccagcacctg gtctcacacc tcgcaaacct cgggttcaac gacgtgatca
      181 tcacgctcgg ctacatgagc gatgcgatcg agaaggcgct cggggacggg tcgctctttg
      241 gcgtcaacgt cacctacgtc cacgagaaga cgaagctcgg gacggcgggc agcgtcaaga
      301 acgcccagag atatctcgaa gagcagccgt tcctcgtcgt cggcggcgac cacgtggtga
      361 gcctgaacct gcttgagttc taccgcgagc acctggcaaa cgactcgctc accaccatcg
      421 gcctcatcag catcgacgac cccaccgagt acgggatcgc cgagatcgac gcaaactacc
      481 agatcaagcg gttcaaggag aagccggggc cgggagagat cttttcaaac ctcgcgagca
      541 ccggcatgta cgtctgcgac ccggagatat tcgaccatat accgaccggc gagaagttcg
      601 actttgcccg aaacctcttc cccaaactca tggaggaggg gcatatcctg aaggcctggc
      661 ttgcccgggg caactggtcc gacgtcggga gcccgcggtc gctccgggag gcggagcggt
      721 ggaaactcca ggagatcggg ttcaccaaca tatccggcga cctctacatc aagggcgccc
      781 ggatcatggg cccggcgcag atagggagct gcgtctcggt tgcggcgaac tcccgggtga
      841 tcgggccggt ctcgataggt gcagggacga tcatcgagga gaacgtcgtc atcgggccgt
      901 atacgagcat cggtgaaggg tgcatcatcc agaacagcgc aaagatcttc tcctcgtcca
      961 tctacaaccg ggtggtggtc gggagggaca gcaccgtcag cggaagcatc atcgacaacg
     1021 atacgctcat cggcagcggg tgcaacatcg agcacgatac ggtcatcggc ccgcacgtcg
     1081 tcctgaaagg cggtgcgatc gtccactccg ggacccggat ctggccaaaa gtcatcatac
     1141 cggaggggac ggtggtgaaa gagcacttct taaacgagga gtacgacacc cggaccgaag
     1201 ggtcctaacc gtgccggaca agccggtggg tcagcgcgac gaggggatgg cgggcgtaat
     1261 cgagcacctt gatgtcgtcg agggtgttga ggttcagggt ctttgccgtc cgctccatcc
     1321 ctaactcgat gttctcgcgg acttcgatga tgtaggcgtc caggttcttg ataccgaggc
     1381 gtttggctgc gatcgcccgg tggtggccgt cgaccaggat ccagcggccc ggcctccgga
     1441 cgacgatcag gggttcggcc agccccttct tgatctcgta catccgtcct tcgagttcgt
     1501 cctcgtatat cttggattgg gtggggagga ggtcgttgat cgggaccggg cctcttcgca
     1561 gcgtcggttc gacgccgtag agtttccgta aggtctcgat gaacttgaag accttgtccg
     1621 gcgagacgtg ctcgatctgg gaccggatga cgtcggcgtt cgatatgatg ccgatgagct
     1681 cgttcttctc gtcgacgacc ggaagcttct ggatgccgga gcggaagatg acccgggcgg
     1741 cgtcgtttat gctcatgtcc gggtcggcga cgatgaggtg gcgggacatc acctgctcta
     1801 ccggtgttgc ggggtgggca aagaggaggt cccgtgccga tatgtagccg accacttcct
     1861 ttgagttctc cacgacaggg aacccgtcgt ggcgcgtcct ctttatggtc tcgatgacgt
     1921 cgcggaccgt cccgtgggcg tcgacggtga cgacgtcgta ggtcatgtag tccttgaccc
     1981 tctttttgtc catcatgata gtgtctcgtt cgcccgagat gaattgttcg gtcgagagcg
     2041 gcgggcgcaa aaaagggtta gtcgatggga atcgtggtcc cgatctcggc cggcaccttc
     2101 ttcaggcgga cctcgaggac gccgttcttg aacgaggcgg cggcgttctc ctcggtcact
     2161 tcggcgggga gcagcaccat gcggctcatc tggccgtaga tgcgttcccg catgaagaag
     2221 tcctggcctt cctctccggt ctcgctcgtc cgcgtgcttg cgatctccag gtgcagggga
     2281 tcggtgatcc ggacggcgac gtcctctttc tcgacgccgg ggagatcggc gacgacgatc
     2341 acctcgtcct cgtggtctct gacgtccacg cgaaagtcgc ggaccgccgg gacgacccgg
     2401 cccctgatct cttcgcctcg cgcgccgatc cccccgagca tggactggaa cctgttctcc
     2461 atctcggcca tgaggtcgtc gaagtcctgc catatcgtgc gatacggtcc tcggtcaatt
     2521 cttgccatgg tccttactcc tgatcgtccg ggatgcccgg gggcatcctg gctaacctta
     2581 aggtagggtt ttaaatatat aaatatattt catggggcat ccgggtgcac acggttcctc
     2641 tcccgtatgg accgtctgca gggggtctcg cccggcccgc cggatgccct gcgaccgccg
     2701 ccgagcactt tgccccacac attccgtttc cgcccggcgc acggcctctc cccggcaccc
     2761 ccccacccgc acgggcggcc ggtgcctcca tccgacgagg gaggagagag gcgaactccc
     2821 cggtggagga gtgccgatcc ggggtggtgc cggcccatcg ggcggcgtgg cagggctccc
     2881 gtggaaggat cgccgcccga taggccgatc tccggtaaaa agagggttct gcccggaggg
     2941 agggaagtat tttatcgggc aggtgccttg agagagcctc tcgaccggac ataatgagtg
     3001 tgccgcggag aggagagggg gatgacttcc ggggacagga ttctggggat gaagatctct
     3061 gagggagacc gcgtggtgtt cgccctctcg ttcgcacgga tgggggatgc cataggaaac
     3121 tccatcctga tcatcgtcat ccccctcctg accgcgcagt tgccggcccc cctcttcccc
     3181 atacccgggc ccgcacgggt cggcgtgttg atcgcgatct acgggctcct ggcagcactc
     3241 gtccagccca tcagcggggt cctcatcgat cgcacgaacc ggcacaaact cttcatcctg
     3301 gtcggtctcg ccatggtggc tatcgccacc ttcggctact cctttgccac cctgtttgag
     3361 agcctgctgg ggctgcgcat actccagggt atcgggtttg cgctgacgat cccggcatcc
     3421 gttgcgatcg tcacgatcgt gacgcagaag atgacccgcg gcggatcgat gggcatatac
     3481 aacagctcgc gatttctggg ctttgccata ggcgccctga tcggggggta tctctacacc
     3541 acctacgggc cgaatatggc tttttatgtc ggtacgggct tcatcatcct cgccatcgtc
     3601 ctggtgcagg ccttcgtgaa ggaggtggag gagccagaag agccccggga tgacccggag
     3661 gtcccggccg gccccataca ccaggaagag aatgcggctt tcgaaggcgg aaagagaacc
     3721 ctgcccggcc tccggggcct cggctttata tccgcgggga tcggcggcct tgcgttcgca
     3781 gtctttctca tggccagcgc attcgccatg ctggtcaccc tggagccgca gttcaacata
     3841 cgcctgcacg agacggcgtt tgccttctcc atcgcgttct ccacccttgc cgcaacccag
     3901 gtggtgttcc agtaccccat cgggagggtg tcggaccggt gggggcgaaa accgttcgtc
     3961 atcgcaggcc tcctgctcat cgccccgacg accgccgccc tcggatttat atctacaacc
     4021 ttccagctcg tcagcctgcg gtttatacag gggatcgcca ccgccgccat cgcggcgccg
     4081 gcattcgcac tcgccggcga cctctcgaag agagggacgc agggccgcca gctcagcgtc
     4141 gtcaccaccg ggttcggcct cggcatcgcg ctcggcccgc tgattgcagg gtttctcgtc
     4201 gtgtacgggt tcgaggtgcc cttcctggtc ggggccgccc tctcgctcct cggggcatgg
     4261 gtggtctacc gctacgtgcc cgagaccgtg gagagggaac ccggcgcccg gtcattccgg
     4321 tggccgggga ggagatagcg cgagggaggt ccactcgccc tcatccaccg gggataacta
     4381 aatatgggaa ggtatcacga gggttgccgg agatcgatgg agcatcgggg agtactgccg
     4441 gcatacctgg agctgaccaa accgaaggtg gtattcctga atctcttcgt aggattggtc
     4501 agtttcctcc ttgctgcatc gcacccgccc cccgttcacc tctgcacgtt tcttgccgca
     4561 ggatacctgg tggcgggcgg gtgcggcgcg atcaactgct ggtatgaccg ggatatcgac
     4621 cgggtgatgg caaggacttc ggggagggtt atccccagga atgccctctc ccctcgccgg
     4681 gttctcctct ttggctctct cctgctgacg acaggactcc tggccgccgt actgatcctt
     4741 ccgttccttg cagccgccat gatggcggcg ggcgcaggca tctacctcat catctatacc
     4801 gcctggctga aacgggcaag ccacctgaac gtggtgttgg gcgggacggc ggtctgtttt
     4861 gccgcgtttg cggggtggac cgcgaccggc gcaccattcc ccacgctcac cccggtgctg
     4921 atcgcgctcg tggggttcct ctggactccg gggcatttct gggcgctcgc catataccgg
     4981 gaccaggatt accgtattgc ccgcatcccc atgctccccg ccagggcagg gcgatacccc
     5041 gcggcgcagg ccatcttcct ctggaacgcg gcaacagcgg gtctcaccgt cctcataccg
     5101 ttcctcacgg cgatcgaccg gatcactctc gccataacga tcccggccgg cctcttcctc
     5161 ctctccttga gcagaaggct tctcgtatcc ccaaccgatg agagggcggt gcgcctcttc
     5221 tccttctcca tcgtgtacct gatgagcgtg ctcttcgcct tcctggtgct ggcggacggc
     5281 ctgccggcgc tgtaggaatc agggtaagcc ggcagcgcac ggtaatatct ctcactcttc
     5341 ctgcccgccg atcgcacccc ttgcgggcgg gacatctcta tggatgtccg gatcggtcga
     5401 acggaaggag aggatcgccg cgaccgcgta gattatcgcg ccgatgaacc acatggccgc
     5461 cccccccatc tcctggtccc cgagcagcgt aacgccgggc aggtgggcgg tcgacgggta
     5521 caggtagacg atctggtcct ggaacgttat gatcgcgccg aggatcatcc cctgcatggc
     5581 gccggcaagg aggtacaacg ccttcccctc gtttgtcctg atgacgggaa ggtgcagccg
     5641 tgacccgggg acaagcggtg cccagtatgc gatgaacgca atgagcaggg tcacgtgctc
     5701 gaggatgtgc acgggcatat cggtgagcgt gacgtcgtac gggatgggta tatgccagaa
     5761 ccacaggacg gccgtggaga ggataaacgc cgtgacgggg taactgatcg cgtagaggcc
     5821 gcggcgaagg gtccggtaca gggtccgctt cctgagcgtt ccgggcggca gccaccacag
     5881 gaagaaatca gcggcaagga gagagggtgc aagtaccccg atcaggatca tatgatagac
     5941 catgtggatg gtgaaggact cccccagggc cggattgaga taggccatgg cgatgagcgt
     6001 cgccaccgcg gtaaaactgg cgaagatctt tgtggatcgt ccggtacggc cccgatcagc
     6061 ctttccattc ccctccatac cctacgaccc cccctccggc agtatatcta cgggaccccg
     6121 ggtgcgttcc caggcccggg gcacccggga cagcatgacg tagcctatcc agataacgcc
     6181 gacaccgatg agaatgatga aaggaactgc gatcgaggcg aatatctgcc actcggggaa
     6241 gtgcacggcg taccgccggg ggatactgga tgccccggat gcaaggaaca taagggtaaa
     6301 cccggccccc ccgaccccgt atgccgccgc ggcataccgc gtgcgggggc tgctcccggc
     6361 tcccgagagg gtgcgaagga tggagaagag gaacccccag aggaagacta ccgcacccag
     6421 gaggaaatag gtgtggaaat ggccgggaat ccagagggtg ttgtgcatca cctggttgac
     6481 ggcgatggtt gcatcgagcg ctgctgccat gcctccaaac atccaccccc agaggccgat
     6541 ggcgagaaag atggtggtta catcccaccg gatgcccgag cggtagtact gggagagccc
     6601 cccaaagagc gtgatcagga tcaccggtat caccgatacg taggttccta tctggcctgc
     6661 tacctgaaac gccaggggct gggcgaaatc ctggtacagg tggtggaggt agatgatggg
     6721 aacgatgaac gtgatggcgt tgagggcgag ggcgtaggta cggctcagtt tccaggcccg
     6781 gccggtgtag gcgggcagaa gaaagtacat cagcccggcg gcgaagtaga tggtcaggtt
     6841 caccaggaga tgcccgaaga gatagtcgaa gttcttcatg aagagcacat cgatgctctg
     6901 gatcagcccg gacgcctgca gccagagcgg gatgagccag gtaaagccgg ctatgacggc
     6961 gacgatcccg gggattgcga cggccaccgc gagcacatcc aggatggaca ggttctcctg
     7021 gcgagcagtc ctccggacga tgaggggcca ccccagcgcg ttgatgactc ccccgtgggc
     7081 gcgggagacc gtgcgggtga tcagcacaca gtagaacagg agcccgacga gcacgaaccc
     7141 tatgccgacc gtcgccccta tcacccccca gaaggcatac cccccatgaa acggcagagg
     7201 gtagagtgcg gtccaggcag ccgcaaaccc tcccccgagg gtgctgaaca ggacaaacat
     7261 catccctata aaaaagaaga agaaggtggc ccagagccag cgggtatcca ggaccaccct
     7321 ccgcccgatg acggcggcaa accccgcaat cgatcctaac agggcgaggg agaccatgga
     7381 gaggccatgc agggtcatga tccggtagaa gagttcgggc cccagcaggt tcaggagccc
     7441 cgactgctcc gtcctcatgg tgaggccgaa gatacccatg atgacgaaca tgacgaaggc
     7501 gaacaccatg ccggtcaggg taaagcggag cacgcctccg tagttggcgg gcagggaggg
     7561 tgtctttacg ggagggaccg catcactcga cattgaaggc tgcctccatt ccccagtggt
     7621 ccaccccgca gtactccagg cagtggatcc tgtacggccc cggccgggtg aaattgacga
     7681 tcaggcggtt ggtatatccc ggcatggact gcgtttgagc gatgatcctc ccgtcggggt
     7741 cgaatatacc gaacccgtgg ttcacatcga tcgatgttac ggcaaactcc accgggacat
     7801 tggcggggat cgtggtgttg ctcaacgtga aggcgaactg cgacgtcgtt gcattgatga
     7861 cgaggacggg ttctcctacc gtgatgtccc tgaccggctg atacggaagg gcgacctggg
     7921 tgcccaggaa cacgacgacg gctgccagga ctatgacgga gtagagccgc cggcgaagga
     7981 gttccagccc ttctgtgata tcatcgtatc cgaccttctc tctgctgctc ctcatcaccc
     8041 atgcccagag gagcgtgtag gacccgccca tcacgagcag aaggacaaac acgtaccgcg
     8101 gatcgatgaa gatcatgtca ggccattacg gtcgggggtt gcacttaagg cttgtggcga
     8161 cgagccccga tcgccctcta tcgccccgga ccgggctgcc atgacgtatt ttccggcgga
     8221 gagcccctcg cgttctcaag cggaaagatc cttatggcag gcaggccgat catactaccg
     8281 gatgagaacg gttgtccgga ccaagacgag caacggcatc gattacctct acgagatcac
     8341 ctgctacgtt aaagcaactg cccgacaaac ctgatcggta cctatggctc ccgtgacaga
     8401 cggcgacacc ctgctcctgc acttcaccag cacccgccct gatggggaga tattcgagga
     8461 tacccgggcc ggcgagccgg tcagggcgac gcttggagag cgccagatca atcccctctt
     8521 tgagaaggcg ctgatcggga gggagccggg cgagacggtg accgtggtgc tgcccccgga
     8581 gaaggcctac gggaagtatc gcagccggct cgtgatcacg atgaagcgca agagactgaa
     8641 cctcgaccac gaaccggtgc ccggcgagtt cctgaaggtg gaagtgatgg gaaagccctg
     8701 cctggtgacc gtcaccgacg tgaccgaaaa gagcgtcacg atcgacgcca accacccgct
     8761 tgcaggggag acgatcacct acgcgataac ggtcgcggca atccttcccc gatccggtta
     8821 ggcccccgtg gtattactcg acgggccacg gttacgcttc tttcccgggg aaagccctcg
     8881 cacccggcgg gttctgttcc atgacccggc aggatgcgcc ggccaaaacc cgtcgacaag
     8941 ctttatgacc ggtgccgtcg atgggcgccg ctgtccggag ttgccattat gaagatagtc
     9001 ggtatcaatg gaagtccccg gggcgggagg agcgccaccc tgcagctcat tgaggcggtc
     9061 cttgagggtg cgaaggagaa gggtgcagag gtcgatgtcg tgcaccttat cgaccttgac
     9121 cttaagttcc gcaacgcctg cagcgcctgt ttccaggagg gcaaatgcgt cgatgaagac
     9181 gacttcccca acctgtatga gaagatcaag gccgccgacg ggcttgtcct cggctcaccc
     9241 gtgtacttcg acctggtcac cggccagatg aagctgctca tcgaccggat ggccgacggg
     9301 atccagtgcc agtcgctcac ggggaagtac gggtgctcgg tcgccacgtc gggcgatcac
     9361 gccgaaccgg cggtcgtcgc gtacctgaat cacttcctcc agatgctcgg cgcaacgccg
     9421 gtcggcaacg tgggggtcgc gatcgggaag gacccgggtg ccctggcaaa ggcaaaagag
     9481 actgctcacg agcttggaaa gacgcttgcc gaagcgatcc agacccggcg gcagtacccg
     9541 cagatcgagg cgttccacaa gcggttccag gagaagttca aagcggtcat cgccggtgag
     9601 aagcccgagt ggccgggcga ttacgatcac tgggtcaacc aggcatgggt ctggtgagcc
     9661 tgcgcccgcc ggcgataccc ggtgagcccg ggcttttcga gtgcggctct cgtcgccccg
     9721 ggatccgggt ttttgagtcg ttcccggccc ccctctaccc ccgggtccag aacgcacgct
     9781 ccggcagcaa ccggccccaa cctatatata gcgtcccgct gcatgggcgc gggggaggcc
     9841 cgcccttccc tgcaggaggt catgtacgat gcccgagttt gcaaacccgt tctccgggaa
     9901 caggatggag cgcaggctgg accggggtga gctcatccgg acgatccggt tctcgatcgc
     9961 cgccgagtac gaggcggtcc agttctacga gcagatagcc gagtcgaccg acgatccgct
    10021 ggtccagaag gtgatgcgcg acgtcgcgaa tgaagagaag gagcatgcag gggagtttct
    10081 gcggcttctc cgggagatcg agcccaccga ggatggattt taccagcacg gctacaagga
    10141 agtcgaggag atgatcgcgg aagtgaagaa gggcggcagg tgatcccctt cctttgaggt
    10201 gccggtattt gcacttgaag ggtcgccgcc gaaccgcccc ggtgccgggc tcccgggcgt
    10261 tcttcgctcg attggccccg ggatcgcgag gcaccgggcc ggcccccttc taccccgcga
    10321 ttcccgggca gtgaacccgc cggcgggcga gccggtcgtt gaggatcacc cgttgaagat
    10381 ttcgggcccg ggtttaaggc accaaccaga atacagaaat accggcggcg ccccacattg
    10441 gagcattaac accctataac cccgatcatc gggaggcttg cgcaatgaag aaaggatttg
    10501 tgacagatat cgagacagag acggtaaaga atacggattt ccgcagggtc ctctacacgg
    10561 ggaagttcag ccagctcgtg ctgatgtgcc tcaagcccgg tgaggagatc ggcgcagagg
    10621 ttcacgacga tgtcgaccag ttcttccggt tcgaggaggg cgagggggcc gtcgtgatcg
    10681 acggcgtgaa gcacgccgtc agggatggca gcgcggtggt tgtaccgagc ggtgcgaacc
    10741 acaacgtcct gaacacctct aagactgccg acctcaagct ctacacgatc tactccccgc
    10801 ccgagcacca ggataaggtc gtgcgaaaga cccgcgaaga ggccatggcc cgggaagagc
    10861 attttgacgg gaagacgacc gagtaatctc ccctgaagcg tataacgatc catctcgctt
    10921 tttccggcgg ccgctgtccc cggtggttat cgggccggcc gccatcgaaa atctccttcc
    10981 cctcatcccg gtcgcgagcc cggcatagcc cgggatatcc gctggcttca cctgcgtccc
    11041 ggccggccgt cacgcgcctc cggctcgcgg tctggttcgc tatcgggcct gcattgcgta
    11101 cgggagccgg cgatggggga agcgaagtga tgcaccggga tacgggagaa gagctgcggt
    11161 agtggcggcg aaggccggga agccttcagg atcgtgggcc gaggagcccc ccttcttctc
    11221 gtcgggggta gttgagatga ggtcacccaa ggagcgtcgt aaccttctgc accagagcca
    11281 cggctgcgct caccgcaaat tctgcctcgg ccgcagatat cgtgcctacg gtgtcgtaga
    11341 ccgaaccgtg tcttttacgg cgcatgcgat caaagacaag ggcgtcaccc tcactgagga
    11401 agagtccggc aaacttcacc accgatatgt gctggtttgc gccgtccgga cgatatccct
    11461 tcgcaaacat aagagcccgg ccagcctgga gggctgcgtt gtaggcgata gtatacgccc
    11521 agtcgcaatc ggtcgaaaga agcgtacgag cggtatcaat atcccggcga gcaagagcca
    11581 tcgcatcagc cacttttttc gggtcgggag ggagcctctg gatcagtccc tgcctctcaa
    11641 gatcctcgat catcgcaaga tccaatgatc atgatctttc tctcgcccat tacattcttt
    11701 acgaaaggat ctccggtctc ccttcgttgt gcaaactcgc tgccgcgcat gagcgtataa
    11761 ttgatctcgc ggttgatcgc gcgttcgcct gtatgcacga gcgggatcag cacctcttcg
    11821 ttcacatcgc ccacgatgaa gaggtcgata tcgctccttc cccctgcggt acctcttgca
    11881 aacgacccgt agatgaacat gcactcgata ttctgcaggg aagagaggtt ctcccggata
    11941 acccgtgcaa ccccttccgt cttgagcacg agttgctgga ggtcggtgta gaggaaaaag
    12001 tcacggttga ccgtgaagta gtgctggttc ccccgccgtc tcccgataag aagcccaaac
    12061 gactccaggt ttgcaagttc gcggcgcacc ccattgatgt tctcgccggt catgcggacg
    12121 atctcgcgga tgtaaaactc gctctccggg ttgaggagaa acagggtgag gagtttcacc
    12181 cgggcctttg atgggatgag cgcttcgagc atgaatacat catgtattca aaaggataaa
    12241 taatgtattc atctgaatac aaaaagtatt cagtctcaca acagatcctg ccggcgcacc
    12301 ctggaaggca ggggggcggc catgccgtcg aaggcggacg aggcagacgg gaagaacctc
    12361 gcaccacggg gccggcatac gagaccgggc tcccgtggag cacccctcac cgttcctccg
    12421 ggctcgaata cgtccgggcc ggggcctgcc cggcgctgca tgccgcctcg acgtctgcaa
    12481 ggtcgtagac gaggaacccc tcctcgcgga gccggccttt cccctcgacg ccgcccgcga
    12541 caaggcagaa gcgctcgtgg gtgtagcggt cgtggcggat ccgctccgct ttctgcacaa
    12601 ggcgcgtgag cacgccgcgg gcttctcgct ccgtgagatc tccccacttg cattccccaa
    12661 agacgatgct cgcgtcgctc tccctggtgg cgacgaggtc gacctcatcg ccgcggtacc
    12721 accaccggcc gatagtgtac cccggaaagg ccgcccgggc gagctcgttt cgcatgaaaa
    12781 cctcgaactg tttgccgaag taggggttcg tatcgatggt atcgatcggg tgattctcca
    12841 tctcgatcgc ctgccggtga gagaagacga agttatacca gaagtcaaag atgcagtcct
    12901 cgatgcggta caacccctgc cgccggctgg cgaggagggg cagctccctc tcgatgatcc
    12961 ggaggcgcgt catcgcctcg aggtaggggt agaggtggcg ggcatccatg ccgcaaaact
    13021 gggcgatggt gcccggggcc gtgttgccga cggcgatggc attgaggatc gcctggtagg
    13081 tcttgagttc ccgaaactcc tgtgagagga ggaagtaggg ctcccggtag aagtagccgt
    13141 acctgttgaa gaactcccgc tcgacgaagg tctcgtagct tctgtagtct ccggccttga
    13201 ggaggtattc ggggatcccg ccgatggtca ggtaggcttt gagagcatcc ccgaaggaga
    13261 acgaagggag gaactgccgt gcatcgcgga accggagcgg cccaaggagc atatcgcggg
    13321 tccgcctccc gtagaggggg gaggtgtagg agagcgcgct gtcggtcatc aacccgagcg
    13381 tcgacccgga gaggaggatg caccagtttg acccggcaag ggcggtgtcc caggctttct
    13441 ggagcgcgga gaggatggtc gggtcgttct tgatcaggta gatgaattcg tctataacga
    13501 ggtagcggcg ctccgtgagc ggtatccggg tgatgtaggt gaagagctga tcccatgtcg
    13561 tgatatcgag cgttgcaagg agcgggtcgg cgaaggaccg ggctatcgcc tcctgaagcc
    13621 gtcggatctg gatctgcggc gcggtgtcct cggcgaaatg gaggataccc tctttcccgc
    13681 ggacgaattc ggtgaccagc cgggtctttc ctatccggcg gcgcccgtag aggatgatca
    13741 gccggccgcc gtccttctgc cactcctcct ccaggagggc acgctcggtc tcgcggtcga
    13801 cgaattccct aatcatgatt agtactaatc tggattagta ttaagggctc cggtgccggg
    13861 gcgaatcagc gggtcaggca cgccggcatt cgtctgacga ccccggcgcg ggatatcccg
    13921 ctgcacgggt ccggcgcaga tacccttatg tggacaaatt gtaaatgcta tttacaatat
    13981 gtccagtccc ccacagagga tgatccggcg ggatgcagag gcgcagatcc ggtcgctcgc
    14041 tcttggcttt ccggcagtgg ccgtcatcgg cccccgccag tcgggaaaga cgacgcttgt
    14101 ccggtcggta ttcccccacc tgccctatgt tttactcgag gaccccgaca cccgggcctt
    14161 cgcggaggag gacccccgca gttttctcat acactacgag aagaccgggg caatcctcga
    14221 tgaggtgcag agggttccgg aactcttctc gtacctccag ggagtccttg acgcgaacca
    14281 gaggccggga cagttcatcc tcacggggtc gcagaacttc ctgatgatgg agcggatatc
    14341 ccagtcgctc gccggtcggg tcgggatcgt caaactcctc cccctctcga tgggagagct
    14401 tgcccgcgcc ggcctcgagg ctgaacgcta cgaggacctc ctctacgccg gcctcttccc
    14461 ccgcccctac agcagtgccg ttcacccgcg agacttctat tcatcctaca tccagaccta
    14521 catcgagcgc gatctccgcc ttctcaagca ggtgcagaac ctctcggcgt tccagacgtt
    14581 catgaagatg tgcgcctacc gatccggcca ggtggtaaac tactcatccc tcgcacatga
    14641 ctgcggcatc acccacaaca cggcgaaaga gtggttatca ctcctcgaga cgtcgatgct
    14701 gatcgttcag gtcaggcccc accgcaagaa cttcaacaag cggcttgtca ggatgccgaa
    14761 actctacttc acggaccccg gtcttgctgc gcacctggcc ggggtgcagg gcgccgacga
    14821 tctcatctac catcccctga ggggagggct ctttgaatcg ctcatcatag cggagttcct
    14881 gaagttccgc ctcaaccggg gtaaggagtc caacctctac ttctggcggg acaaactcgg
    14941 gcacgagatc gactgtatca tcgagtatat ggggtgggat cccatcccgg tcgagatcaa
    15001 atcgggccga acgccaagcc ccgacttctt caatgagatc gcctactgga acgaactctc
    15061 cggcaacgcc ccggatcgtt cgttcgtggt ctatgggggc gatcggtccc agcgtcgggc
    15121 ggcaggacac ctggtcgggt atcgagacct ggaaccaatc atccggtacc tggagtgagg
    15181 ggaggccggc ggcatgcacc ggattccagg agcatccaga ccggtgcccc tggagagcgc
    15241 ggtatactcc tccctcttac gctccccgca ccgctcggca tgcgccatca gaccactccc
    15301 gtgacagagt ctttccgctc ctccccccgc agcacccccg ccgcatagca ggagagcgcc
    15361 atctccacat ccgccgcggc ccagataccc tcgctccgga ggcccgcggc ggcaaacccc
    15421 gccgggtgcg ggcaggagac ctcatcgcgg ttgagaccgc ccgcaaccgc ctgcagggcc
    15481 cctgcccacg ctccgtactc ccccggccac cccggttgcc cggcagggat cgcccaccga
    15541 tccccggaag gctccacggc gaggtcgagc aggggatagc gctggctccc ggagtcgccg
    15601 cggccaaaga ccctgatcag ccgggcgtcg agcgtgccaa agacctgcgg gacggcgaac
    15661 cggaggagtt tgctcgcaca taccggcccg agcccccgga tctgtctctc gacggtctgg
    15721 atggtctcct ccggctccag catcagccag tatgccgggg cgctgccgat gtagagggtg
    15781 acggcgatcc gggccggggc cgagagatcg tcccggcggg cgatcttctg cacgtggtcg
    15841 agcccgagcg ccaggctccg cgcggcggcc gtcacctccc gctcaaggtc gaggatctcc
    15901 aaaaacccct tctcccaggt atcgtcccgc cacgtgtatc cccggtagag ggcgggaaag
    15961 tcgagatcgt cgagcagccg gccgacctgc tcctccatta cctcacctcc ggagagggtg
    16021 ccccgccgat cctctctgcc cggcggggaa atttcaggcg gtagtcgacc gccggcggcc
    16081 gcatcaaccg gtcgtcgatc ttctccctcc ggtagccctt gacgtagccg tcgattgcgg
    16141 ccgtgagccg gacgatatcg ccgcggcgaa ggtctcccac tgcgtcgaac ccggcggtgt
    16201 agttgatccg ggcgcggtcg gcaaggtcgc ggacctgccc gagcaggatg gaccgcccgg
    16261 cgcatcccct gccccggcag gccccgtact gccggaagac ggcggtcccc cgccgcccgg
    16321 caagcccctg aaggttggtt ctcatggttt gatacctcct acagatgagc atgcgccgga
    16381 tcgaaacatt aacaacttca cgtgtattca gtgaatattg tgatgagcat gaccactaag
    16441 aacgatccat tgccgggggg cctgcgcgac gtcgtccaca tcatccccct cggccacgag
    16501 atcgaccggg cggtggcgcc gttcacgaag aacaaggccg accgcgccta catcctcgcg
    16561 gtcccgccgg acgccggcct cgacccccgg atggtcagaa agcagcacta cttcgtcggc
    16621 cgggtgacgg agcagctgca ggatctcggt atcgccgtga cgttcgtccc ggtcgccatg
    16681 tttgatatac tcgacgtcct gaaggtcgtc tcttacctga tccgcaccga aaaagaggcc
    16741 ggcaacgccg tttacgtcaa catgtcctcc tgcggccgga agacctccgt cgcggtgacg
    16801 ctcgccgcga tggtccacga ggccatgctc tactatgtca gcgccgaccg ctacgcgacg
    16861 gcggacggtg aagagtgcga gcacggcctc tcgatcgtca agaccgtcag gacagaagtc
    16921 tttcagcctt tccggatcat gatgccgcgg cccgagaacg tcaggctcct cgtcgagctc
    16981 ttccggcgca aagaggaagg cggcatgacg agcgaggaga tcatcgagtt cttccacgac
    17041 gcaaacaccc acggctacga gaggctgcac acggccgagc gggacggcta ccagcgggca
    17101 aaaaagaagc gggcgctcct gaaccggacg aaccgcggct acctgaagga actcgaggag
    17161 cagggttacg tggaacggga gtggcggggc cggcacttct cgatccggat cacgaaggcc
    17221 ggggagcata tcgcgtgcgt gagcgggctg gttcccggcg ggtgactgcc cggtagtgcc
    17281 cacgagatcg ataccgcaga tacagccacg cagagccgcc cctccccccg acgccgacgg
    17341 caagcggg
//
LOCUS       DAOSEE010000031        17261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1346_length_17261_cov_210.943101, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000031 DAOSEE010000000
VERSION     DAOSEE010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 17261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17261
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1346_length_17261_cov_210.943101"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PLI76_03860"
     CDS             complement(<1..326)
                     /locus_tag="PLI76_03860"
                     /inference="COORDINATES: protein motif:HMM:NF020897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOI61199.1"
                     /translation="MTKQISTMAAEKGDPPIPFPGSEDGAARILEVLKEETRGLSISD
                     ISRRINLNRSSVAKYLGMLVTAGRVEMYVIGSAKVYRISSRPSLLEVFDYIQESVIVI
                     DSDLTVR"
     gene            complement(642..1316)
                     /locus_tag="PLI76_03865"
     CDS             complement(642..1316)
                     /locus_tag="PLI76_03865"
                     /inference="COORDINATES: protein motif:HMM:NF019407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin N-terminal domain-containing
                     protein"
                     /protein_id="HOI61200.1"
                     /translation="MVVKKVQGVTEHAVSPVVGVMLMLVATIIIAAVVSSFGTGLTGD
                     VDTASNARVELVGVSLGGGVRNTLGPVGVVFKNAGGGSLDLRNLRFYATGSAMNSQGS
                     VTLTYNDPVSLSYAGIKNNYDVRPPEEAKGYRMQKFGSGLTSEDLKDPIVEPGERFII
                     YAEYYKAYYAPAVFLGFKADRGDTDTATWSSGAIAVNGGSHYTLSDINTGTVYSEGYL
                     EPEGVF"
     gene            complement(1310..2038)
                     /locus_tag="PLI76_03870"
     CDS             complement(1310..2038)
                     /locus_tag="PLI76_03870"
                     /inference="COORDINATES: protein motif:HMM:NF019407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin N-terminal domain-containing
                     protein"
                     /protein_id="HOI61201.1"
                     /translation="MYRGGRYPREKSDPPFFTENERAVSPVIGVMLMLVVTIIVAALA
                     SSFAGGLSDSAEPAPTTAFDINIPAGENTGVRASAGSLAPPAPGCVVLTMIAGEALHS
                     KDLQIIMTYTVPETYNGVPTTNAGKIIKHTLDGALGSTNTWSAGDDPFIPQVHGYPKG
                     TSKTGIGLPPGNGMVGVGTTYFGTGLFKVGEQYWFKDRNAFLGFDVDDRAAYGFQEGS
                     VVHVTIIHTPSGQTVYDKDVVATW"
     gene            complement(2067..2723)
                     /locus_tag="PLI76_03875"
     CDS             complement(2067..2723)
                     /locus_tag="PLI76_03875"
                     /inference="COORDINATES: protein motif:HMM:NF019407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin N-terminal domain-containing
                     protein"
                     /protein_id="HOI61202.1"
                     /translation="MIEKAYNGGDDAVSPVVGVMLMLVVTIIIAAMVSAFSGTVVQDQ
                     TLAPQVTLSVSYASSITDTNRANSIPDHPAAFKPNNGIKFTLSGGESFSLKDVTVQLK
                     NGDSVMNFDMNTHLNATAAVDTEKITIVANSEGNNTYFGLPGGGDELITVGDSFMIVA
                     DNCYDNTLSADSVKGRFLTWSPKSSTGTFRVQVDVPFEYAIIDQLSGKPMQRGTIIIR
                     "
     gene            complement(2720..3367)
                     /locus_tag="PLI76_03880"
     CDS             complement(2720..3367)
                     /locus_tag="PLI76_03880"
                     /inference="COORDINATES: protein motif:HMM:NF019407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin N-terminal domain-containing
                     protein"
                     /protein_id="HOI61203.1"
                     /translation="MNKQIRNGDSMNSDKNDAVSPVIGVMLMLVVTIIVAAFVSVFAG
                     SAFGNTETTPSAAIDVKIMSNGGLNKDQYVMLIEHHGGSSIPTSDMRIISHYTPPTSK
                     EKGMQRGEITTSTTAVGITVNGEAVPSVKVPYLNDVSRGKPGAAGTNFGEYTFSSGDV
                     LSTGSSVGTQKVLGFDMTTAAKRAEFGFERGSAVDVEIIHLPSQKSLYSSRVIVQ"
     gene            complement(3364..4014)
                     /locus_tag="PLI76_03885"
     CDS             complement(3364..4014)
                     /locus_tag="PLI76_03885"
                     /inference="COORDINATES: protein motif:HMM:NF019407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin N-terminal domain-containing
                     protein"
                     /protein_id="HOI61204.1"
                     /translation="MVNREKNDAVSPVIGVMLMIVVTIVIAATVSVFSTGFASETHSA
                     PNTQIEYVGVLTGPIGGLGEIGLVFENKGGEALFLPDLEFHLKSTMSGGDEVSISYTD
                     LTSVTYRGVSEPGEARLSSSIANRMKKIGASGKNDDVIADARVKAGDRFVIYADRYTN
                     DGASRYGLIVYVADRARPGAPYSSGEFEVSSRTTYTLSDKNTGAVIASGTLVGSVL"
     gene            complement(4008..4691)
                     /locus_tag="PLI76_03890"
     CDS             complement(4008..4691)
                     /locus_tag="PLI76_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin N-terminal domain-containing
                     protein"
                     /protein_id="HOI61205.1"
                     /translation="MQFRRKTPPSGETEQPGPARWDDAVSPVVGVMLMLVVTIIVAAI
                     VSSFAGGLGSTSGTAPAATLAIKMFAGPSDKNVTIEHLGGDPLATKDLQIISTYTVPS
                     RWDQGQGANTLTNAGRVIKHTIDGSLAPIPENALDWSADPFTPQVTNDDSIVSERTAD
                     QTFGTAVLVPGGRLTFERDNFLGFKTGKRGTDGKSGFGFGEGVITHVMIVHTASGKVL
                     YDKDVVVPW"
     gene            5088..7430
                     /locus_tag="PLI76_03895"
     CDS             5088..7430
                     /locus_tag="PLI76_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61206.1"
                     /translation="MNFSAFSDPVKGGPVRMVRIDSGGQQTDPIALINGIANYIRGNP
                     GQYYPVYSDGTVNQGRYCLVQDVAGSLGQVMIRTFGTDVEPVPNPSRQNPIPYRMGVQ
                     FLLPDHTLPYSEFQGPWYEYELQGNVKTSSITNVAGDTVSLAAFSADPAAWNDTFVFR
                     FSDQSGVSRNTQVTMVFRMTLNGLNHEVPYTFTVRDYPPAIILNEMTVQRSGDLSFTV
                     QGTPFMQYDITLPDPPAGEEYPVFSGGGWDSRFSDYHVRVHPGWNGTVRLQVHIPDTA
                     PVTSYTIRATGPDIVQPVTATFRIDQKIMTLVFDRPEKQLYAIGDTILLSGGLTNLAA
                     SNSPSRIPIYLYVTGPNLPPNGAPLTDLSRSVEDGVPATFTVTEYNPLFGTWAYLWDT
                     SQISCDAGTYTVHANLQPIGHRNSGYPGSPGSIDGEVPPSWDYELNMPSLHAKFDEET
                     GGVFARGDYLYSWWYGRGSPGHTSTSSGHMKWYIFGPNFKYADYNPRFPLEDDGAYGI
                     TLPRSFTYDLSPGDYFIVYHHPGTDNQFDVLPENNLYFRGSVNGLYSTADGSLIANVG
                     MLDGRAAVEALVRALDSSSMNDLYMMDTFTVENPVVRINPVGELVVGDELIVSGTTNL
                     AGKGETADKTDVHDDLILTIAALDLYESGKANTAMKIPINETIPREYDPDLGARPFYY
                     MDPIDTSSWYPGTYQITITCKEVNYKTTSTFELLREGSQRNTTADAAQNPPPSRMTVT
                     PGSVVGAPEFTPIPIPTQSPGFTVYLVVAAIFGVFAARRR"
     gene            7433..9112
                     /locus_tag="PLI76_03900"
     CDS             7433..9112
                     /locus_tag="PLI76_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61207.1"
                     /translation="MDLAFDESLFKKIIAVLVVAIVIVGVVAVLRFSGIGFGQEPGPV
                     VPDPYVSDPGIDREPPEETLTGPKSYTWSYGGTERTMHLYIPDDTYQRFISSSYGSHD
                     EKPQRMAEYVVTDGDDGIIGAAADWFLATSRASGFGDSGTVSNVLAFVASLNYTTDEE
                     RLATSDYPNYPVITLATGEGDSEDHAILAAAILDKMGYGVGLLYYPATHDRRTIIPEA
                     TALALINDGTVPGRLYRTVAEAPAGRFIYYPENGTYTGTLPQGGDPASGWYSGSAVGY
                     NVTSAEVLGDIRYYPENRTLVAGTKALDAHMVAVEDAAWNQPVIVSAAWTVNTTEKGV
                     PRSAYDGMEPFFAGGDGLWLGYILSRDDRLDVDTTVAGKQPLGEAPPFSANTSLTAAL
                     RIPISEPSPPPMTWLESVQDYYTSTWYPAGLSWTYDDRWKLHENFLAMQDPLLRNPGT
                     YSLQGVTEVVAPVPWRITYTVRNMDVARSEKGMTPYSDVRFALYRIGDGVAVFDRTFG
                     WQTVYGAEMRKIEAVFGPGDYAVAVFVRNCKVDVAIEYHGKPAKTAYRGGI"
     gene            9109..10659
                     /locus_tag="PLI76_03905"
     CDS             9109..10659
                     /locus_tag="PLI76_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system ATPase subunit"
                     /protein_id="HOI61208.1"
                     /translation="MKFDLSSLFGKKTKRTPAGDNGAADEVPDPTPLILTLPPADPTV
                     DMVDRYWLVPPFSYANIFRRDPITLQYEIVEPRITSKELVVLEETFEQLRATLIYDTA
                     RKRGELGLDTDLIKKVIRSFYPEIADDRMELLVYYLRRNFLGYGKLDPLMNDDRIEDI
                     TCNGPGLPVFLYHRRYASIQTNCTFDGEDLNKFVLKLAQKADKQLSLSTPLIDASLPE
                     GSRAQITYSDIVSSRGSSFTIRKFRAEPMTPVSLIENDTYDADLMAHIWLAVENRKSM
                     VISGGTASGKTSTMNAISFFIPPVAKIVSIEDTREIQLPHVNWLPMRTRESANVSGTG
                     NVGMFHLLKAALRQRPEYIIVGEVRGEEAQTLFQAMNTGHTTYSTVHAGNVQETVNRL
                     THEPINVPVAMFNALDLILVQSLLYDGGRGFRRCLSLNEIRVVDDEIRWEPLFTWDHR
                     TDRFVRVYERSSVLDEIAYRNGWSNEQLEAAITVRRDALVDTVQRGASTSSMVGQMIQ
                     EMMVQEQQ"
     gene            10656..12452
                     /locus_tag="PLI76_03910"
     CDS             10656..12452
                     /locus_tag="PLI76_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOI61209.1"
                     /translation="MTKSAMLDNFRDELAHSLLSAHIPIPASRYLQYGLIVTLLAGFL
                     YIFSALLLSLFGIEANILPFLPYGVTVLLGFIAAVGLLLLAICLYPLLLAQGRRTRIE
                     MDLPHAVTYMQALSSTLTLYGIFRSVYEAGDLYGEVSKECGLIVRDVEVFGLDLLTAM
                     RNVQEVTPSANFKELINDLGLVYRSGGNLTNFFNSKSETYRELARQEQESLLQILEMV
                     AEVYVTAFVAGPIAIIIMLVAQNLAGQSQLSGIMPLMYLGLPLGAIALIFILYVLLPP
                     DNLDVARREIRESEFGVEILDTDAGDKPDPTFLKSLESRKRWLRFLEVLRHPGTFFIS
                     DYTVGAVIGTGLLVALFLSYRFGSFGSIFPSYTLEVFLCLAVIAAVIPVMIAYELRRR
                     YVMAVEKQLPEFLAEIADMRDIGMTLQGAIFMISNTRMGVLSHEIKVAAEELRFGSSV
                     SSALVRMEERIGLVSVKRAISLLVRASEVTDYIREILTIAINDLQHYIKMKAKRLNVS
                     FVYLAVIYLSFGIYLYSAYQLNVAFISSFSAYDVTFDISANVREMLHVGIILGFFSGI
                     MAGQLSSNSILAGFKHVCLMLAATVILFVYII"
     gene            12462..12911
                     /locus_tag="PLI76_03915"
     CDS             12462..12911
                     /locus_tag="PLI76_03915"
                     /inference="COORDINATES: protein motif:HMM:NF019407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin N-terminal domain-containing
                     protein"
                     /protein_id="HOI61210.1"
                     /translation="MKDGIQYDEAVSSVVGEMIMVALVIILVALFATSAFSLIPGGRE
                     ASVDVAMKNASNGTTSAGDTTVIFWHKGGDWVEENDLAVVVIKENGVRDPWKIVKLHD
                     HAGNPTGAFDLGGYLVVEPKPGSSPIGNNDTVRLVTQKSVAYSGVIP"
     gene            12908..13756
                     /locus_tag="PLI76_03920"
     CDS             12908..13756
                     /locus_tag="PLI76_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61211.1"
                     /translation="MMEKDDAVSTVIALMLVLGIVSTLVAIYSAAYLPGLKQQSEIEH
                     SREVAGAFARFDSDIQHVVSHRTSARFSEPFSLGGGDILLSPVRSSGSLTIRAAEGPL
                     VNVTVVNGTQTVTKAATLANISYVPSFTTWEPQGYLWEYGFVAVTKDDVTVPQSSSYN
                     TVSEARAGTDSGEFLRAFVDLTPKGGGPLTITLVKLVPKEGGSFVTGSGRVALGLSAT
                     VSGETIEEVTGITFSFDPEVPGMQDYLDEKCGQVSLSYIPGSNTLDIPGQPVAFRIVN
                     VEVSVW"
     gene            13750..14247
                     /locus_tag="PLI76_03925"
     CDS             13750..14247
                     /locus_tag="PLI76_03925"
                     /inference="COORDINATES: protein motif:HMM:NF019407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin"
                     /protein_id="HOI61212.1"
                     /translation="MVDREAAVTPVVGVILLLAIVIILAALVASFAGGAADAKDPPPS
                     TDLAVYPAGSEEKFTLVFEHRGGDQVRLRDLRINTWVHLPDGGLCAASHEQEKLEGLF
                     GTDIWRAGAGSNTGDLEDTAGFLGLEEEKLREYVGRSAVVEVAIYHLPSGALLHKSSV
                     LLKER"
     gene            14253..14777
                     /locus_tag="PLI76_03930"
     CDS             14253..14777
                     /locus_tag="PLI76_03930"
                     /inference="COORDINATES: protein motif:HMM:NF019407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin N-terminal domain-containing
                     protein"
                     /protein_id="HOI61213.1"
                     /translation="MGYAGTLFDDAVSEVIGVMLMLSVTLIVVALVAVYATGAAGDTT
                     QPIKADLIASGTMKDGDKYQVVFEHRAGDAFDTDQLAVSIGSREDPALHMILRNDDPR
                     DRITCFDPEESIVQLGDRFKVYLGEYDDTDKQVTYREEGEVLYTIPYGCHLTYRFVDR
                     RTGAPISSGEFLIK"
     gene            complement(15029..16369)
                     /locus_tag="PLI76_03935"
     CDS             complement(15029..16369)
                     /locus_tag="PLI76_03935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOI61214.1"
                     /translation="MFAFVLMSVYQYLKDYLYPEISLADSHLHTVVFTTLLATAAAYF
                     VLRKQQALLAMLVAEANERRETGEALRTSEEKFRSIVETANEGILQIDSAYTTTYVNR
                     TMAGMLGYPTGEMLGRPLADFVVAGDLDVYWSRMAAREQGLDGRYECRLRHSDGSIRC
                     FLVSATARKDGDGRFAGSFAMFTDITERKRTEEALQRHTEDLARLHQQLAAANREANL
                     YLDILTHDIKNTENVANLYSDLLIDTLDGDARIHAEKLQRSIRKSIEILGNVSKIRRI
                     HQPSGGRKQMDLDAVVRGEIAQFPEATIRYAATGCRVLADDLLSEVFANLIGNAVKFG
                     GPEVEIAIRVDDEGEFFRVSVEDTGPGVPDDQKEEIFHRYEKRRRGIGEGLGLFLVQV
                     LIERYGGRIWVEDRIPGYPDSGAVFRFALQKAGDDQPEADPMTARGSAGFPGTG"
     gene            16848..17246
                     /locus_tag="PLI76_03940"
     CDS             16848..17246
                     /locus_tag="PLI76_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61215.1"
                     /translation="MHIASGTKLFGFGILTWLLPFLVSVPFYSPEGEPLYDIFLIKSV
                     LLVFSAALGTALILVYFRGVRARYVREGALLGGVWLAINWALDVAVLLPLSGMDIGAY
                     MGEIGLRYLMIPIIAVGIGYAAEQAVRGRG"
BASE COUNT         3366 a         5286 c         4947 g         3662 t
ORIGIN      
        1 cgcaccgtaa gatccgaatc gatgacgatc acgctctcct ggatgtagtc gaagacctcg
       61 aggagagagg ggcgggagga gatgcgatac accttcgccg agcctatgac gtacatctcg
      121 acccggcccg ccgtcacgag catacccagg tacttggcca ccgagctgcg gttgaggttg
      181 atcctccggg agatgtcgga tatcgaaaga ccgcgcgtct cttccttcag gacctccagg
      241 atccgtgccg ccccatcctc ggagcccggg aacggtatgg gggggtcgcc cttttctgcc
      301 gccattgttg agatctgttt tgtcaaacgc aatatttatt gctttagttc aaattaattt
      361 gttctatgcc catgggagag ataatgagca ccttgccacg gtgccggacc ccgttctcct
      421 gaaaaaccga tgagggtctc cggagaaacc gtacgccgtc cgaccccgtg cagctccggg
      481 gcctcccgga gagccgggtt ttctcccggc ggcgaaattt cgtggaaaaa gagggcggga
      541 gggaaaggga tatcccgtgt tccccctctc ctgcgtggct tctccgcctg ggtttatgag
      601 ggagcctcct ctctcatggt cgggcagcgc agcatccccg attagaatac accctccggc
      661 tccaggtatc cctcggagta caccgtgccg gtattgatgt ccgagagagt gtagtgagaa
      721 ccgccgttga cggcgatggc accgcttgac caggtagctg tgtctgtgtc tccacgatcg
      781 gctttgaagc cgaggaatac ggctggagcg tagtatgctt tataatactc agcataaatg
      841 atgaaccgtt ccccgggctc cacgatcgga tccttcagat cttcggaggt caggccgctc
      901 ccgaatttct gcatgcggta tccttttgct tcttccggag gccgaacatc atagttgttt
      961 tttattccgg cataggaaag gctgacggga tcgttatagg tcagcgtaac cgatccctga
     1021 ctgttcatag cggaacctgt cgcataaaat ctcaggttcc ggaggtccag ggagccgccg
     1081 ccggcattct taaacacaac tcctaccggc ccaagtgtat tccttacacc ccccccgagg
     1141 gacacgccga ccagctctac ccgggcgttc gatgccgtat cgacgtcgcc ggtaaggccg
     1201 gttccaaacg agctgacaac cgcggcgatg atgatcgtcg ccacgagcat cagcattacc
     1261 ccgacgacgg gggagacggc gtgctccgtg actccctgca cttttttgac taccatgttg
     1321 ccaccacatc cttatcgtat acggtctggc ccgacggtgt gtgaatgatc gtcacgtgga
     1381 cgacggaccc ctcctggaac ccgtacgctg ccctgtcgtc tacatcgaac ccgaggaacg
     1441 cattacggtc cttgaaccag tactgttcgc ccactttgaa caggccggtg ccgaagtagg
     1501 tcgtgcctac accgaccata ccattgccgg gtggaaggcc aatacctgtt ttggaggtac
     1561 ctttcgggta gccgtggacc tgcgggatga aaggatcgtc acccgcactc caggtgttcg
     1621 tgctgccgag cgcgccgtcc agcgtgtgct ttatgatttt tcctgcgttt gtcgtgggca
     1681 ctccgttgta agtctccggc acggtgtagg tcatgatgat ctggaggtct ttggagtgca
     1741 gggcctcccc cgcgatcatg gtcagcacaa cacatccggg agcaggagga gcaagcgagc
     1801 cagcggaggc tctcaccccg gtattttctc ctgcaggtat gttgatatca aacgccgtcg
     1861 tcggtgccgg ctcggccgag tcactaagac cgccggcaaa gctgctggcg agagcagcga
     1921 cgatgatcgt cacgacgagc atcagcatga cgccgatgac cggtgagacc gcacgctcgt
     1981 tttcggtgaa aaaaggagga tcgctttttt ccctcggata tcttccgcct ctgtacattg
     2041 ttctgtcacc ctgtaatggc aggcaatcac cgtatgatga ttgtacccct ctgcatgggc
     2101 ttgccgctca gctggtcgat gattgcatac tcgaagggta cgtctacctg taccctgaac
     2161 gtaccggtcg agcttttggg agaccaggta aggaatcttc cctttacgct gtcggcggag
     2221 agcgtattat cataacagtt atcagcgacg atcatgaacg agtcccctac ggttatcaac
     2281 tcgtcgcctc ctccggggag tccaaagtat gtattgttac cttcggaatt cgccacaatc
     2341 gttattttct ccgtatcaac cgccgcggta gcgttgaggt gcgtgttcat gtcgaagttc
     2401 atgacgctgt ccccgttctt cagctggacg gtaacatctt tcagcgagaa cgactctccg
     2461 ccggagaggg tgaacttgat cccgttgttc ggtttgaagg ctgcagggtg gtccgggatt
     2521 gagtttgccc tgttcgtatc cgtaatgctg gaggcgtacg atacggaaag ggtaacctgc
     2581 ggtgcaagcg tctggtcctg cacgacggtc ccggaaaatg cgctcaccat ggcagcgatg
     2641 atgatcgtta cgacgagcat cagcatcacc ccgacgaccg gcgagaccgc atcgtccccc
     2701 ccgttatacg ctttttcgat cattgtacaa tcaccctgct gctgtagaga ctcttctggc
     2761 tcgggagatg gatgatctcg acgtcaaccg ccgagcctct ttcaaaacca aactctgcac
     2821 gctttgctgc cgtagtcata tcaaagccaa gtactttctg tgtgcctacg gaacttccgg
     2881 tactgaggac gtcgccggaa gagaacgtgt actccccgaa gttcgtgccc gccgctcccg
     2941 gttttccgcg tgagacgtcg ttgaggtagg gcactttcac ggacggaaca gcctcgccgt
     3001 tgacggttat acctacggcg gtcgtcgagg tcgttatctc gccacgctgc atacctttct
     3061 ccttcgatgt cggcggcgtg taatgactga ttatcctcat atccgatgtt ggaatactgc
     3121 ttccgccgtg gtgctcgatc agcataacgt actgatcttt gttcaatccg ccgttggaca
     3181 taatcttcac gtcaatggca gccgaagggg ttgtctccgt gttcccaaag gcacttccgg
     3241 cgaaaaccga tacaaatgcg gcaacgatga tcgttacgac gagcatcagc atgacgccga
     3301 tgaccggtga taccgcatcg ttcttatcag aattcatact atctccgttc ctgatctgtt
     3361 tgttcataac acacttccca caagagtgcc ggaggcgatg actgccccgg tatttttgtc
     3421 gctgagcgtg taggtcgtcc tgctgctcac ctcgaactcg ccgctggagt atggtgcacc
     3481 cggtctcgcg cgatccgcga catatacgat aagcccgtac ctgctcgcac cgtcgttggt
     3541 atagcgatct gcatagataa caaacctgtc tccggcctta accctcgcat ccgctatcac
     3601 atcatcattt ttaccacttg caccgatctt tttcatgcgg ttcgcgatgg atgacgaaag
     3661 tcgtgcctcg cccggttcgg aaaccccgcg atacgtcacc gacgttaagt cggtgtacga
     3721 gatcgagacc tcgtctcccc cggacatggt cgacttcagg tgaaactcca agtcgggcaa
     3781 aaacaacgct tctccgccct tattctcgaa caccaggccg atctccccga ggccgcctat
     3841 cggccctgtc aatactccga cgtactcgat ctgtgtattg ggtgcggagt gcgtctccga
     3901 ggcgaacccg gtggagaata cgctcaccgt tgccgcgatg acgatagtaa cgacgatcat
     3961 cagcatcacc ccgatgaccg gggataccgc atcgtttttc tcccgattta ccacggtaca
     4021 accacatcct tgtcgtagag gaccttcccg ctcgcggtgt gaacgatcat cacgtgtgtg
     4081 atgacccctt cgccaaatcc aaatccagat ttaccatcgg tcccacgctt gcccgtttta
     4141 aacccgagaa agttgtcgcg ttcgaacgtg agccgcccgc cgggcacaag aactgccgtc
     4201 ccgaacgttt gatctgcagt tctctcggag acaatgctgt cgtcgttggt tacctgcggg
     4261 gtaaacggat cggcactcca gtcgagggcg ttttccggga tcggggcaag cgatccgtcg
     4321 atggtgtgtt taatcacacg cccggcgttc gtcagtgtat tcgcaccctg accctggtcc
     4381 catctacttg gaacagtgta cgtgctgatg atctggagat ccttcgtcgc caggggatcg
     4441 ccgcccaggt gttcgatggt cacgtttttg tcgctcggtc cggcgaacat cttgattgcc
     4501 agcgtcgccg ccggcgccgt gccgctggtg gaccccaggc ccccggcgaa actgctgacg
     4561 atcgcggcaa cgatgatcgt tacgacgagc atcaacatca cgccgacgac cggcgagacc
     4621 gcatcgtccc acctggcagg gccgggctgc tctgtttctc ccgatggcgg tgtttttctc
     4681 ctgaattgca tgtccatgtt ctctcccgtt tacaaaatta atttagttat ctcaaatttt
     4741 attgcaaaaa cacatgtccc ccatatatta ttaacttttc taaatacctg gtataagacc
     4801 gtatttcgaa gaactgtgcc tttcaaagcc gcatttcgga gatgcaatgc acatttgcat
     4861 cgcaacaaag tgaaatagcg ctataacaaa taaatttgat aatgtggtgc atccccgtct
     4921 caaggtgtct caataacatg ggcagatatc ggatcatggt gatactaacg ataacgctcc
     4981 ttctcctggt tgcttccgcc ccgtcggttt cggcacggtt tgtgacccct gaaggcccca
     5041 acgccgggat cagtcccggg gatgccgttt ttcagggaga gaggaccgtg aacttctcgg
     5101 cgttctccga cccggtaaaa gggggcccgg tgcggatggt caggatcgat tccggggggc
     5161 agcagaccga cccgatcgct ctcatcaacg gcatcgcgaa ctacatccgt ggaaatcccg
     5221 gccaatacta tcccgtttac agcgatggga cggttaatca ggggagatac tgcctggtgc
     5281 aggacgttgc cggcagtctc ggacaggtga tgatccgcac ctttgggacg gatgtcgagc
     5341 ccgtcccgaa ccccagccgg cagaatccca tcccttaccg gatgggggtg cagttcctgc
     5401 tccctgacca caccctccca tacagcgagt tccagggccc atggtacgag tacgaactac
     5461 agggtaacgt caaaacttcg agcatcacga acgttgccgg ggatacagtc tctcttgcgg
     5521 ccttctccgc ggatcccgca gcctggaacg acaccttcgt cttccggttc tccgatcaga
     5581 gcggcgtctc ccggaacacc caggtgacga tggtcttccg gatgaccctc aacggcctca
     5641 accacgaagt accgtacacc ttcacggtgc gggactaccc gcccgcaatc attctcaatg
     5701 aaatgaccgt gcagcgcagc ggagatctct ctttcacggt ccaggggacc cccttcatgc
     5761 agtacgacat cacccttccc gatcccccgg caggggagga gtaccccgtt ttctccggcg
     5821 gcgggtggga tagcaggttc tccgattacc atgtgcgtgt ccaccccggg tggaacggca
     5881 cggtccggct gcaggtccat atcccggaca cagctccggt caccagctac acgatccggg
     5941 caaccggccc cgatatcgtc cagccggtga ccgcgacgtt caggatagac cagaagatca
     6001 tgacattggt cttcgatagg ccggaaaagc agctgtatgc catcggcgac accattctac
     6061 tgtccggagg attgaccaat ctagcggctt caaactcgcc atcccgcatc ccgatctacc
     6121 tctacgtcac gggacccaac ctgccgccga acggcgcgcc cctgaccgat ctgtcgcggt
     6181 ctgttgaaga cggcgtccct gccacgttca cggtgaccga gtacaacccc ctctttggaa
     6241 cgtgggcata tttgtgggac acctcgcaga tctcctgcga tgcaggtacc tacaccgtcc
     6301 atgccaacct ccagccgatc gggcaccgca atagcggtta tccgggatcc cccggctcca
     6361 tcgacggaga agtcccgccg tcgtgggact acgaactcaa tatgccgtcc cttcatgcga
     6421 aattcgacga agagaccggc ggggtgtttg cccgcgggga ctacctgtat tcgtggtggt
     6481 acggccgcgg aagccccggc catacctcca cttcctccgg gcacatgaag tggtacatct
     6541 tcggcccgaa cttcaagtat gccgactata acccccggtt ccccctcgag gacgacgggg
     6601 cctacgggat caccctcccg cgaagtttta cctacgacct ctctcccgga gactacttca
     6661 tcgtctatca ccaccccggg acggacaatc agttcgacgt cctgcccgag aacaacctct
     6721 acttccgggg gagcgtgaac ggactgtaca gcacggccga cggcagcctg atagcgaacg
     6781 tcgggatgct tgatggtcgg gccgcggtcg aagccctggt acgggcgctg gactcgtcgt
     6841 ccatgaacga cctctacatg atggatacgt tcacggttga aaatccggtc gtccgaatca
     6901 acccggtcgg ggagctggtc gtcggggacg aactgatcgt cagcgggaca accaaccttg
     6961 ccgggaaagg agaaaccgcc gataaaacgg atgttcacga cgacctgatc ctgacgatcg
     7021 ccgcgctcga cctctatgag tcgggcaagg cgaacaccgc gatgaagatc ccgatcaacg
     7081 agaccatccc ccgggagtat gacccggacc tgggggcgcg gccgttctat tatatggacc
     7141 cgatcgatac cagttcctgg tatccgggga cctaccagat caccatcacc tgcaaggagg
     7201 tcaattacaa gaccaccagc accttcgaac tcctccgtga ggggagccag cggaatacca
     7261 cggccgatgc ggcgcaaaac ccgcccccct cccggatgac ggtgacaccg ggctccgtcg
     7321 tcggggcgcc tgagtttacc ccgataccca tacccacgca atctcctgga tttacagtat
     7381 atcttgtagt tgccgcgata ttcggggtat tcgccgcaag gaggcggtag ccgtggatct
     7441 cgcctttgac gaatcactgt tcaagaagat catcgcggtg cttgtggtcg ccatcgtcat
     7501 cgtcggcgtc gtggccgtcc tcaggttctc cggcatcggt tttgggcagg aacccggccc
     7561 cgtcgtcccg gacccctacg tgtcagatcc cgggatcgac agagaacccc cggaggagac
     7621 gctcaccgga ccaaaatcct acacctggtc gtacggcggg accgagagga cgatgcacct
     7681 ctacataccc gacgacacct atcagcggtt catctcctcg tcgtacggat cgcatgatga
     7741 gaaaccacag cgtatggcgg aatacgtcgt gaccgacggc gacgacggga tcatcggcgc
     7801 cgccgcagac tggttccttg ccacatcccg ggcatcgggg ttcggcgaca gcggcaccgt
     7861 ctctaacgtc ctcgccttcg tggcaagcct caactacacc acggacgagg agcgtctcgc
     7921 gacttccgat tacccgaact accctgtcat aaccctcgcc acgggagagg gcgacagcga
     7981 agatcacgct atccttgccg ccgccatcct cgataagatg ggctacggcg tcggtctcct
     8041 ctactacccg gcaacccatg accggcgcac gatcatcccc gaggccaccg cccttgccct
     8101 gatcaacgac ggcaccgttc ccgggcggct ctaccggacc gttgcggagg ccccggcggg
     8161 caggtttatc tactatccgg agaacggcac gtataccgga acccttcccc agggcggcga
     8221 tcccgcatcg ggatggtact ccggcagcgc tgtcggatac aacgtcacct ccgcagaagt
     8281 tctcggcgat atacgttact atccggagaa ccgaacgctc gtcgccggaa cgaaagcgct
     8341 ggatgcccat atggtcgccg ttgaggatgc cgcctggaac cagccggtca tcgtctccgc
     8401 ggcctggact gtaaatacca ccgagaaagg cgttccgcgc agcgcatacg acgggatgga
     8461 gccgttcttt gccggcggcg acggtctctg gctgggctac atcctctcgc gcgacgaccg
     8521 actcgacgtc gatactaccg tcgccggaaa gcagcccctc ggggaggcgc ccccgttctc
     8581 ggcgaacacg agccttacgg cggctcttcg catcccgatc tctgagccct ccccgccgcc
     8641 gatgacctgg ctggaatcgg tacaggacta ttacacgagc acctggtacc ctgcggggct
     8701 ctcgtggacc tacgacgaca gatggaaact ccacgaaaac ttcctcgcga tgcaagatcc
     8761 cctgctccgg aacccgggaa catattcgct ccagggtgtt accgaggtcg tcgccccggt
     8821 gccctggcgg atcacctaca cggtgcgcaa tatggatgtg gcccgttccg agaaggggat
     8881 gaccccgtac agcgacgtcc ggttcgcgct ctaccgcatc ggagacggtg tcgccgtctt
     8941 tgaccggacg ttcggctggc agacggtcta cggtgcagag atgcggaaga tcgaggcggt
     9001 cttcggcccc ggagactatg cggtggcggt cttcgtccgg aactgtaagg tcgacgtggc
     9061 tatcgagtat cacggtaaac cggcaaaaac agcctaccgg ggtggaatat gaagttcgat
     9121 ctctcctccc tgtttggaaa aaagacgaaa agaacccctg ccggggataa cggcgctgca
     9181 gatgaggtcc ctgaccccac cccgctgatc ctgacccttc cgcctgcaga ccccaccgtg
     9241 gacatggtgg accggtactg gcttgtcccc cccttctcct acgccaatat cttccgcagg
     9301 gacccgatca ccctgcaata cgagatcgtc gaacccagga tcacatcgaa ggaactggtc
     9361 gtccttgagg agacgttcga acagctccgg gcgacgctga tctacgatac cgcccgcaag
     9421 agaggcgaac tcggcctcga tacggacctg atcaagaagg tgatccgctc cttttacccg
     9481 gagatcgccg acgaccggat ggagctactc gtatactacc tccggcgcaa cttcctcggc
     9541 tacggtaaac tggacccgct gatgaacgac gaccggatcg aagacatcac ctgcaacggc
     9601 ccggggctcc cggtctttct ttaccaccga agatacgcca gtatccagac gaattgcacc
     9661 tttgacggcg aagacctgaa caaattcgtg ctcaaactcg cccagaaggc tgacaaacag
     9721 ctctcgctct cgacgccgct catcgatgcc tcccttccgg aggggtcgcg ggcacagatc
     9781 acctactcgg acatcgtctc ttcccggggg agctcgttca ccattcggaa gttccgcgcg
     9841 gaacccatga ccccggtcag cctcatcgag aacgacacct acgacgccga cctgatggcg
     9901 catatctggc tcgcggtcga gaatcggaag agcatggtca tctccggagg aacggcaagc
     9961 ggaaagacct cgacgatgaa cgccatctcg ttcttcatcc ccccggtggc aaagatcgtc
    10021 tccatcgagg atacccggga gatccagctt ccgcacgtca actggctgcc gatgcggaca
    10081 cgggagagcg caaacgtcag cggcaccggc aacgtcggca tgttccatct cctcaaagcg
    10141 gcactgcgtc agcgccccga gtacatcatc gtcggtgaag tgcgcggcga ggaggcgcag
    10201 accctctttc aggcgatgaa caccgggcac accacctact ccaccgttca tgcggggaac
    10261 gtgcaagaga cggtgaaccg cctgacccat gaacccatca acgtcccggt ggcgatgttc
    10321 aacgctctcg acctgatcct ggtgcagagc ctcctctacg atggagggag agggtttcgc
    10381 cggtgcctct cgttgaacga gatccgggtc gtcgatgatg agatccggtg ggaacctctc
    10441 ttcacctggg atcaccggac tgaccggttc gtcagggtgt acgagcggtc gagcgtcctt
    10501 gatgagattg cctaccggaa cggctggagc aacgagcaac tggaggcggc cattaccgtt
    10561 cgaagggatg ccctcgtgga tacggttcag agaggtgctt ctacctcctc gatggtcggg
    10621 cagatgatac aggaaatgat ggtgcaggaa cagcaatgac gaagtcagca atgctcgata
    10681 atttcaggga cgaactggcg cactcgctgc tctcggcgca cataccgatt cccgcctcgc
    10741 ggtacctgca gtacggcctg atcgtaaccc tgcttgccgg cttcctctac atcttctccg
    10801 ccctcctcct ctccctcttc gggatcgagg cgaatatcct ccccttcctc ccctacggcg
    10861 tcaccgtcct gctcggcttt atcgctgccg tcggcctgct gctccttgcg atctgcctct
    10921 accccctcct tctggctcag gggcgccgga cacggatcga gatggatctc ccccatgccg
    10981 tcacctacat gcaggcgctc tcctcgaccc tgacgcttta cggtatcttc cggagcgttt
    11041 acgaggcggg cgacctctac ggcgaggtat cgaaggagtg cgggctcatc gtcagggacg
    11101 tggaggtctt cggcctcgat ctcctcaccg cgatgcgaaa cgtccaggaa gtcacgccgt
    11161 ccgctaactt caaggagctc ataaacgatc tcgggctggt ctaccggagc ggcggcaacc
    11221 tcaccaattt cttcaactcc aagtccgaga cctaccgcga acttgcgcgg caggaacagg
    11281 agtccctcct ccagatcctg gagatggtcg ccgaggtcta cgtgaccgca tttgtcgccg
    11341 ggccgattgc gatcatcatc atgctggtgg cgcagaacct cgccggacag agtcaactct
    11401 caggcatcat gccgctcatg tacctcggcc tcccgctggg ggcgatcgcc cttatcttca
    11461 tcctctacgt cctccttccg ccggacaacc ttgatgtcgc gcgccgcgaa atacgggaat
    11521 ccgagttcgg tgtcgagatc ctggacaccg atgccggtga taaaccggat ccgacgttcc
    11581 tcaaaagcct cgagtccagg aaacggtggc tgcgttttct tgaagttctc cgccatcccg
    11641 ggactttctt catctccgac tacacggtcg gggcggtgat cggcacgggt ctcctcgttg
    11701 cgctgttcct cagctaccgg ttcggctctt tcggatcgat ctttccctct tacaccctgg
    11761 aggtcttcct ctgccttgcc gtcatcgcgg ccgtgatccc ggtgatgatc gcctacgagc
    11821 tgcgccgccg ctacgtcatg gcggtcgaga agcagctgcc cgagtttctc gccgagatcg
    11881 cggatatgcg cgatatcggc atgacgctac agggcgccat cttcatgatc tcgaataccc
    11941 ggatgggcgt tctctcccac gagattaagg tcgccgccga agagctcagg ttcgggtcca
    12001 gcgtctcgag cgcactggtg cgcatggaag agcgtatcgg cctggtctcg gtgaagcgcg
    12061 cgatctccct cctggtacgg gcaagcgagg tgaccgatta catccgcgaa atcctgacga
    12121 ttgcaatcaa cgaccttcag cactacatca agatgaaggc gaagcggctg aatgtttcgt
    12181 tcgtctacct cgcggtcatc tacctctcgt tcggcatcta cctctactcg gcgtaccagc
    12241 tgaacgtcgc cttcatctcg agtttctccg cgtacgacgt caccttcgac atcagcgcaa
    12301 acgtccggga aatgctccac gtcgggatca ttctcgggtt cttctcgggg atcatggcgg
    12361 gccagctctc ttcgaacagc atacttgcgg gtttcaagca tgtctgcctg atgcttgctg
    12421 caacggtgat cctctttgtt tacatcatat gaggcggttt catgaaagat ggaatacagt
    12481 acgacgaagc ggtctcctcg gtcgtcggcg agatgatcat ggtggcgctg gtcatcatcc
    12541 tcgttgctct cttcgccacg tcggcgttct ccctgatacc gggcggtcgt gaggcgagcg
    12601 tcgatgtcgc gatgaagaac gcgtcgaacg gcaccacctc ggcaggggac actacggtga
    12661 tcttctggca caagggcggc gattgggtgg aagaaaatga tctcgcggtg gtcgtcatta
    12721 aggaaaacgg agttagggac ccttggaaga tcgtgaaact ccacgatcac gccggcaacc
    12781 ctactggggc gttcgatctc gggggatatc tcgtggtcga gcccaaaccc ggttcttctc
    12841 ctatcggcaa taatgatacc gtacggctcg tcacgcagaa gagcgtcgcc tactcggggg
    12901 tgatcccatg atggagaagg acgacgcggt ctcgaccgtg atcgcgctga tgctggtcct
    12961 cggcatagtc tccacgctgg ttgcgatcta ctccgcggcg tacctccccg gattgaagca
    13021 gcagtccgag atcgagcact cccgcgaggt ggcaggcgct tttgcccggt tcgattcgga
    13081 tatccagcac gtcgtatcgc acaggacgag cgcccggttc agcgaaccgt tctccctcgg
    13141 cgggggtgat attctcttaa gcccggttcg gtcgagcggt tcgctcacga ttcgggcagc
    13201 ggaggggcct ctggtgaacg taaccgtcgt caacgggacg cagaccgtga cgaaggcagc
    13261 cactctggcg aacatctcct acgtgccgtc gttcacaacc tgggagccgc agggatacct
    13321 ctgggagtat gggttcgttg cggtgaccaa agacgacgtg acggtacccc aatcgtcgag
    13381 ttacaacact gtatccgagg cacgggccgg gaccgacagc ggcgagttcc ttcgcgcgtt
    13441 cgtcgacctg accccgaaag ggggcggtcc cctcacgatc accctggtga agctcgttcc
    13501 gaaagaaggc gggtcgttcg tcaccgggag cgggagggtt gcgctcgggc tgagtgcaac
    13561 cgtctccgga gagaccatcg aggaggtgac cgggatcacc ttctcctttg atccggaggt
    13621 gcccggaatg caggattacc tggacgagaa gtgcgggcag gtgagtctgt cgtacatccc
    13681 gggctcgaat acgcttgaca ttcccggcca gcccgtggct ttccggatcg tgaacgtcga
    13741 ggtgagcgta tggtagaccg ggaggcggcg gtcaccccgg tcgtcggggt gatcctttta
    13801 ctcgcgatcg tgatcatcct cgccgcgctc gtcgcctcgt ttgccggcgg ggctgccgat
    13861 gcgaaggatc ctcccccctc gacggacctt gcggtgtatc ctgccgggag cgaagagaag
    13921 ttcactctcg tcttcgagca ccggggcggg gaccaggtcc ggctccggga tctcaggatc
    13981 aacacctggg tccacctgcc ggacggcggt ctgtgtgctg cgtcccatga gcaagaaaag
    14041 ctggaggggc tcttcggtac cgatatctgg agggcggggg ccggttcgaa taccggggac
    14101 ctggaggata ccgccgggtt tctcggcctc gaagaagaaa agttgaggga atacgtcggt
    14161 cggtcggccg tggtcgaggt ggcgatctac cacctcccga gcggggctct cctgcataaa
    14221 agcagtgttc tcttaaagga gcggtgaatg gtatggggta tgctggaact cttttcgatg
    14281 atgccgtgtc agaggttatc ggcgtcatgc tgatgctctc tgtgacgctg atcgtcgtgg
    14341 ctctcgtggc ggtgtacgcc accggtgcgg cgggtgatac gacgcagccg ataaaggcgg
    14401 acctgatcgc ctccgggacg atgaaggatg gggacaagta tcaggtcgtc ttcgagcaca
    14461 gggccgggga tgcgtttgat accgatcaac tggcggtgag catcggctcc cgtgaagatc
    14521 ctgccctgca catgatactc aggaacgacg atccgcgaga tcgtataaca tgctttgacc
    14581 cggaggaatc gattgttcaa ctcggggatc ggttcaaggt ttatcttggc gagtacgacg
    14641 acaccgacaa acaggttacg tacagggagg agggagaggt tctgtacacg atcccgtacg
    14701 gctgtcacct gacctatcgc ttcgtcgacc gccgtaccgg cgcacccatc tcctcaggag
    14761 agttcttgat caagtaagct tgcaccatcc tgtttttgga ggattgcagg gtttgagccg
    14821 gtctcctggt acaacagcag ccctgcggtc gccggtctcc gggagcatcg gcgggctttc
    14881 atagggcgcg acgagcctta cgaacgccga tacggcgagg ctcacgacga ggagggcgat
    14941 gaggccggag gaaggaaggc gagggggatg ggagatttga ggacggtgca ggcagcgtcg
    15001 cgatctccta taccacgggg cgtttgcctc agccggtccc gggaaacccg gcgcttccgc
    15061 gtgccgtcat cgggtcagcc tccggttggt cgtccccggc tttctggagc gcaaagcgga
    15121 acaccgcccc tgaatccggg tatcccggta tgcggtcctc cacccagatc ctgccgccgt
    15181 agcgctcgat gaggacctgc acgaggaaga ggccgagtcc ctcgccgata cccctcctcc
    15241 tcttctcgta gcggtgaaag atctcctctt tctggtcgtc gggaaccccc gggccggtat
    15301 cctcgaccga gacccggaag aactcgcctt cgtcgtcaac ccggatagcg atctcgacct
    15361 ctggcccgcc gaacttgacg gcgttgccga tgaggttcgc aaagacctcg gagagaaggt
    15421 cgtcggccag cacccggcaa ccggtcgcag cgtagcggat ggtggcctcg gggaactgcg
    15481 cgatctcacc gcggacgacc gcatcgagat ccatctgctt gcgccccccg gacggttggt
    15541 ggatccgccg gattttggag acgttcccga ggatctcgat gctcttcctg atactccgct
    15601 gcaacttctc cgcgtgtatc cgcgcatccc cgtccagggt gtcgatcaga aggtcgctgt
    15661 agaggttcgc gacgttctcg gtgttcttga tgtcgtgggt aagtatgtcc aggtagaggt
    15721 ttgcttcgcg gttcgctgcg gcgagctgct ggtggagccg ggcaagatct tcggtgtgac
    15781 gctggagtgc ttcctcggtc cgtttgcgct cggtgatatc ggtgaacatg gcaaaagacc
    15841 cggcaaacct tccgtcccca tctttccgtg cagtggcgga gacgaggaag caccggatgc
    15901 ttccgtcact gtgcctgagg cggcactcgt atctaccgtc aaggccctgc tctcttgctg
    15961 ccatgcggct ccagtatacg tcgaggtccc ctgcaaccac gaaatcggcc agaggccggc
    16021 cgagcatctc cccggtcggg tagccgagca tgccggccat cgtacggttg acgtaggtgg
    16081 tcgtgtaggc gctgtcgatc tgcaggatcc cctcgtttgc cgtctctacg atcgagcgga
    16141 acttctcctc gctcgttcgc agggcttcac ccgtctccct cctctcgttc gcttcggcaa
    16201 ccagcatcgc aagaagggcc tgctgtttac ggaggacgaa ataggcagca gctgtggcga
    16261 gcagggtggt gaagacgacg gtatggaggt gcgagtctgc gagcgagatc tcggggtaga
    16321 ggtaatcctt gagatactgg tatacgctca tcaggacgaa tgcgaacact cccacccttg
    16381 cgagagtgag atagaggttg gggggttgta actcgttatt catgcgcgct ccacggagaa
    16441 gcggtacagg atgttctgca tgtcgggaga tcgctgatct acggagatat cgtggaatgt
    16501 tcggccctgg ctgatccatc ggcctgaata ctgcctgtat cggccaggtt tcgcgttccg
    16561 gggctggacc ggagtgacag catatatgcg gtgcctgatt gcattctccc ctgagatttc
    16621 ataaaccttc cgtcggtggc gggccgaccg taatcacgat cctttgttaa tggtgcagaa
    16681 gctttagcga accgcgggga ttctccggaa gaacccgccg gcaacccgcg ctacgctgct
    16741 ttccggtgca tccgttccgt gacggcgccg gcagggagga ctattgccct gctttctccc
    16801 ggcgtatgtc cgattggtat attcgcgccg gagtatcatc ctcatccatg cacatagcgt
    16861 caggcacgaa gcttttcggc ttcggcatcc tcacctggct ccttcctttc ctcgtatccg
    16921 tccccttcta ctcgccggag ggcgaaccgc tctacgacat cttcctgatc aagtcggtcc
    16981 tgctcgtctt ctcggccgcg ctcgggactg ctctcatcct ggtctacttc aggggtgtgc
    17041 gtgcccggta tgtccgggaa ggcgccctgc tcggcggcgt atggctcgcg atcaactggg
    17101 cgctcgatgt cgccgtcctc ctgccccttt cggggatgga tatcggcgcc tacatgggag
    17161 agatcgggct gcgttacctc atgatcccga tcatcgcggt cggtatcggg tatgcggcgg
    17221 aacaggccgt ccggggaagg ggataacctt cccgcacctg c
//
LOCUS       DAOSEE010000032        17233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1350_length_17233_cov_266.371114, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000032 DAOSEE010000000
VERSION     DAOSEE010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 17233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17233
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1350_length_17233_cov_266.371114"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..832
                     /locus_tag="PLI76_03945"
     CDS             119..832
                     /locus_tag="PLI76_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI61216.1"
                     /translation="MLSIRVVRQLRDFSLDVALSVRCGETLVLIGENGAGKSTVLNLI
                     SGILAPDRGDITLGERTLFSSAARIDIPAESRNIGHLFQSYALFPHMTVAENVAFGLR
                     CRKVPKPEIAGAVAEQLRSMHLSDLADVNVGRLSGGQRQRVALARALVLEPELLLLDE
                     PLAAVDMRARGAMREELRDRIRHAGIPCIVVTHTLHDVLGLADRLCLIEEGRVATEGT
                     PEEVLGMRENGFIASFIEG"
     gene            2299..2463
                     /locus_tag="PLI76_03950"
     CDS             2299..2463
                     /locus_tag="PLI76_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61217.1"
                     /translation="MGEVAIATASPMIAVCGVPIGLAGIGTLALIGTVISVLLAAAGL
                     TRQRVRSRKG"
     gene            complement(2467..3324)
                     /locus_tag="PLI76_03955"
     CDS             complement(2467..3324)
                     /locus_tag="PLI76_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HOI61218.1"
                     /translation="MRTIELRPDQPLDLDLTLSCGQAFRWENVDGWWQGVVDGRAVRI
                     RQDADRLTFEGADAAFVRDYLRLDQDLPAILASIDRDPVIGAAVRECRGLRLVRQQPW
                     ECLASYICATNTNIPAVKRRVALMAERYGRPTDGPSGMTYAFPEPEALAEASRTDIWD
                     CKLGYRTDYICEAARFASENPGWAERIAALPFEDARRALMEFKGVGPKAADCVLLFAF
                     GFFEAFPVDVWIRRIVGETYLSDLAGRSCTPAEYERIRRFSRDYFGEYAGYAQEYLYC
                     ARGPARRQR"
     gene            complement(3346..4260)
                     /gene="nifB"
                     /locus_tag="PLI76_03960"
     CDS             complement(3346..4260)
                     /gene="nifB"
                     /locus_tag="PLI76_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase cofactor biosynthesis protein NifB"
                     /protein_id="HOI61219.1"
                     /translation="MADESYRTATVQGREVPYDPEQLRKISEHPCYSDKACHAFGRCH
                     LPVAPKCNIQCNYCVRDFDCVNESRPGVTSKILTPEEGLDLVRKVVKDYPFVKVIGIA
                     GPGEPLANPETFEALRLVHEEFPHLIMCLSTNGLMLPESIDELAKYDVGNVTVTLSAI
                     DPAIGEKIYSWVEYKGKKYRGREAAELLLSQQLKGIEMAVAKKMFVKINTVYIPGIND
                     EHIPAIAKKVGEMGAFTFNVIPLIPQYKFADITPPTAKDKREMQDRCAPFIKQMRHCA
                     RCRADAIGKLGQDVQSCVYQQMKDEKAE"
     gene            complement(4388..6379)
                     /locus_tag="PLI76_03965"
     CDS             complement(4388..6379)
                     /locus_tag="PLI76_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOI61220.1"
                     /translation="MTDEIHTDEPPDSRRKGRTRDKRTIGPVPNLEEHVKSDWWRRIF
                     NHLYLKTDGDVVDDQRITGREIDRVARVLRLGPDEKILDLCCGQGRHTLELARRGYNA
                     EGLDQSHYLIQRARSTAKREGLQVRFREGDARRLPYRTDSYDVVLVLGNSFGYFDSVE
                     EDLRILTELRRILKPWGQVLLDVADGEYLKEHFQPRSWEWIDRTMFVCRERSLSLDGQ
                     RLISREVITDVDKGVVADQFYAERLYTRDALRQLLERAGFSRIAFHEIATESQRNQDL
                     GMMERRHIVTAAIRKEWAKAKAKGQERAKHVAVMLGDPTKADALKPSCVFDDDDFYTI
                     DQMKAALRELKGYRFTYLCKHDTMIPDLTKLKGKVDYIFNLCDEGFNNDPRKELHVPA
                     LLEMLDIPYTGSAPQSLAFCYDKSLVRGIAKEMGIPVPDACFITPGDRTYDVGMKFPV
                     IVKPNSGDSSYGITQRSIAHTIEELSDIISSLRTTLGYSQSLLVEEFLTGKDISVGII
                     GNPSGYYTVLPITEEDYSALPPELPRICGYEAKWLPDSPYWKIVSRPANLPEETEKVV
                     VRCCLALFERLECRDYCRFDWRLDEHGNPKLLEVNPNPGWCWDGHLAKMAKYAGLSYP
                     DMLGRVLKAAEERFGMDQETGAREAEAKTVPLDLSRQEA"
     gene            6701..6877
                     /locus_tag="PLI76_03970"
     CDS             6701..6877
                     /locus_tag="PLI76_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61221.1"
                     /translation="MDERYSGRGPRSFQDRPREFHKAVCSDCGKACEVPFKPTEGRPV
                     YCRDCLPKHRKPRY"
     gene            6920..7096
                     /locus_tag="PLI76_03975"
     CDS             6920..7096
                     /locus_tag="PLI76_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61222.1"
                     /translation="MEERYPSRGPRSFQDRPREFHKAVCSDCGKACEVPFKPTEGRPV
                     YCRDCLPKHRKPRF"
     gene            complement(7217..7984)
                     /locus_tag="PLI76_03980"
     CDS             complement(7217..7984)
                     /locus_tag="PLI76_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI61223.1"
                     /translation="MIDITGRVWSVWRRNRDAFLKTYRVNFIPPFVEPVLYLLALGFG
                     LGTYIESVDGIPYPVFIAPALVSISVMYSSFFECTYSSFVRMYYQKTFDAIIATPVSI
                     DEVIAGEMFWGATRGMIYAALMLPVLLLFGVVAMPSSLLLIPFAYLAGLLFATIGMCF
                     TAITPGIDALNYPAFLFITPMFLFSGTFFPLDLLPEPIRYFALAVLPLTHVVSINRAI
                     TLPAFAPANLISLAWIAVATAFFFVLAIRLMRRRLIV"
     gene            complement(7992..8900)
                     /locus_tag="PLI76_03985"
     CDS             complement(7992..8900)
                     /locus_tag="PLI76_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI61224.1"
                     /translation="MGDVIVARDLEKRFPDLTAVDRINFRVREGEVFGFLGPNGAGKT
                     TTMKMIQCISPKTGGTLEVLGMDVDTRQREIKGRLGVVPQENNLDPDFSAYQNLLIYA
                     RYFGIPKREAERRAEELLTFMQLEEKRDVLIDRLSGGMRRRLIVARALINAPELLILD
                     EPTVGLDPQARHLIWEKLRSLRAEGNTLVLTTHYLDEAERLCDRLVIMDHAKILVEGA
                     PADLIREHAGSNVVEAERTPAVLTCLDEFGLDYDLAGGAIQVFTDHPHDVARELLETC
                     RHEAVTVRPATLEDVFLRLTGRSLRE"
     gene            9244..9690
                     /locus_tag="PLI76_03990"
     CDS             9244..9690
                     /locus_tag="PLI76_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HOI61225.1"
                     /translation="MAECAEEKGARMDRAIYPSDIVYVGSTEKRGRGVFARRDISAGE
                     LIEVCPVIAMDETDGQRLLDRTGLFNYYFAWGRHEESAAVALGYGSLYNHSYHANADH
                     VRDVAGGEIRIYACRAIRGGEEITINYGGLPDCPDPVWFDVVEEER"
     gene            complement(10394..10711)
                     /locus_tag="PLI76_03995"
     CDS             complement(10394..10711)
                     /locus_tag="PLI76_03995"
                     /inference="COORDINATES: protein motif:HMM:NF022420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2769 domain-containing protein"
                     /protein_id="HOI61226.1"
                     /translation="MDAFEQFAQKLMKMPLIARIDALKAERAKCICMNCPTYTECAEK
                     LDQGFFCFTGMSIICIDREVECICPTCQVPKDTGLQHTAFYCTRGDEKARRYDRSLAE
                     KTK"
     gene            complement(10900..11109)
                     /locus_tag="PLI76_04000"
     CDS             complement(10900..11109)
                     /locus_tag="PLI76_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HOI61227.1"
                     /translation="MKLSARNQLPGKVTKIDTGVVTAEVTIALDGGGEITSVITKKAV
                     ENLGLAVGKKVYAVVKSTEVMVAID"
     gene            11461..13038
                     /locus_tag="PLI76_04005"
     CDS             11461..13038
                     /locus_tag="PLI76_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61228.1"
                     /translation="MSTMRPLRIAILLFCLILTVPAATAGNLTILQPSANESTFAEMR
                     DFYVYGVFPEPLEHPGNIRLVLTDESGVIVRTVMSCVNETGVTPEAAVEMSLVPNRWG
                     GLLAPDVITEPGGIANGSNKVLVTGTYYLALVQGGVTRGMGSYTDASGGMLAPITRDL
                     TAGTYTLVVEGLDGDLAGERVEKELVFGTTHASLGTFRPDANRENVVGWARERVPPLR
                     VYIDWFPGYFRSGDAGYEIPDRWQANNGIEVANDCTGTTVDTIASARNDCLIYNVGST
                     SATLQVEIAAILRNGLVDSDRTTFTHYDIGEPSMAWVDRATGAAESMIGRQVAYPAGT
                     RLVYTRAEITDEPVRTNYFNTVANDSSKTVDTTPMQVTVVQGENLTFYGVTRPIETTL
                     TGAGSAYRYTPDDRIATYIYENETLGRFTFTGCLEREFEGGYIGAAGYEFGHAFTETA
                     AMPAGTWLFAVSAYDTTGTPVEMTNRTIELEVAAPPALPTATLVAAAAPAPTRTSGFW
                     SVPALVALLAAVAARRR"
     gene            13439..13624
                     /locus_tag="PLI76_04010"
     CDS             13439..13624
                     /locus_tag="PLI76_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61229.1"
                     /translation="MPTKTVVERKKATPKAAPKKALTPAEQAERQASIDRLDADIRKS
                     KMGKSYTLAEIKKEFGL"
     gene            complement(13619..13933)
                     /locus_tag="PLI76_04015"
     CDS             complement(13619..13933)
                     /locus_tag="PLI76_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61230.1"
                     /translation="MTKYEIVFKESAKKDLKVIERDDGEYVSKILEKIEFCLGEHLFD
                     RIGQCSKKKMEGKEKAHRLHVQKRYTIFYTVERTKEKTFVEVHLIMSYAAAHKRYKRI
                     DL"
     gene            complement(13943..14101)
                     /locus_tag="PLI76_04020"
     CDS             complement(13943..14101)
                     /locus_tag="PLI76_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61231.1"
                     /translation="MKRINAKGEVVQQTSIYTRENAYLLAKEASIPMSEAAEQGVIAA
                     LRARGVEV"
     gene            complement(14441..14514)
                     /locus_tag="PLI76_04025"
     tRNA            complement(14441..14514)
                     /locus_tag="PLI76_04025"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14478..14480),aa:Val,seq:tac)
     gene            complement(14727..15674)
                     /gene="mch"
                     /locus_tag="PLI76_04030"
     CDS             complement(14727..15674)
                     /gene="mch"
                     /locus_tag="PLI76_04030"
                     /EC_number="3.5.4.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018759 - methenyltetrahydromethanopterin
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HOI61232.1"
                     /translation="MLSVNELALDIFEELFEYAEEFHAVPHEFDNGARIVDCGVSTTG
                     GYLAGRRFTEICMGGLGEVDITMGRIRDVPIPFIEVSTDFPAIACLGAQKAGWTVNVN
                     KYFAMGSGPARALSLKPKHTYEVIDYEDEFDYAVIALESDHLPNAGVMEKIADACDVD
                     VANTCAVVAPTASIVGSVQVAGRCVETAVYKLNELGFDTRKITAGIGHAPIAPVKKDG
                     TRAMGSTNDATIYHGSIMLTMNAPEIKDYLDRIPSNKSRGYGKPFYEIFKEAKFDFYQ
                     IDTSLFSPAEVVINELSEGAVYHVGAVNPDVTLKSFGFI"
     gene            complement(15724..16872)
                     /locus_tag="PLI76_04035"
     CDS             complement(15724..16872)
                     /locus_tag="PLI76_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORC1-type DNA replication protein"
                     /protein_id="HOI61233.1"
                     /translation="MKRSLLMWDETLFRDPEVFEIDYVPEQFNHRDTQIRELAFQVRP
                     GLRGGRPLNTICRGLPGTGKTTSVRKVFAEIEEVTQKLVPIYINCQIDNTKFAVFSQI
                     YRRIFGHLPPASGVSFKQLLDAIARALQREEQVLLVGLDDANYLLYENEINHVLYSLL
                     RSHEAYPGVRIGVVAIVSDMSVSLQTEVDARVASVFRPTEIYFPPYSEEEIRGILGER
                     VVQGLYPNVLRSDLLDLVVEQTTKSGDLRVGIDLLKRATLSAEKAARRSIEQEDICKA
                     YKVSRYLHLAYSLRALRAEEREVLGRIAEMAIRDDRETNAGEVYHFVREHVGISYTRY
                     YKMIQKFDAMRLLNLHYRQGRGRTRLISLRYDPDRVLEHLRREQTGSI"
     gene            complement(16947..>17233)
                     /locus_tag="PLI76_04040"
     CDS             complement(16947..>17233)
                     /locus_tag="PLI76_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61234.1"
                     /translation="RVLEPPARPEKKVSVQPETPRVLEPPARPEKKVSVQPETPRVQA
                     PQQETPTAKPPGAPADDVCEICGAEVTKSQAKLSQLFMSKKLCKKCMEHP"
BASE COUNT         3290 a         5118 c         5255 g         3570 t
ORIGIN      
        1 cgatctatac gacgatgcag ggagacatgg acgcctcgat cagccttgca atcatcctcg
       61 tcgtgatatc gttcgcggtg atcgccgcgg tgaagatcgt cacccggagg agcgcctgat
      121 gctctccatc cgcgtcgtcc ggcaactacg ggatttctcc ctcgacgtcg cgctctcggt
      181 tcggtgcggc gagacccttg tccttatcgg ggagaacggg gcgggcaagt ccacggtgct
      241 gaacctgata tcgggcatcc ttgcgccgga ccggggcgat atcaccctcg gggagaggac
      301 gcttttctcg tctgcggcgc ggatcgatat ccctgccgag agcaggaaca tcggccacct
      361 cttccaatcc tacgccctct tcccgcacat gaccgtcgct gagaacgtgg cgttcggcct
      421 ccggtgccgg aaggtgccga aacctgagat tgccggcgcc gtcgcggagc aactccggtc
      481 gatgcacctc tcggacctcg cggacgtcaa cgtcggccgt ctctcgggag ggcagcggca
      541 gcgggtggcc ctcgcccggg ctctggtgct cgaaccggaa ctcctcctcc tcgacgagcc
      601 gcttgcggcc gtcgacatgc gtgcccgggg agcgatgcga gaggaactcc gcgaccggat
      661 acggcacgcc ggcatcccct gcatcgtcgt cacccacacc cttcacgatg ttctggggct
      721 cgccgatcgc ctctgtctca tagaggaggg gagggtggcg accgagggaa cgccggagga
      781 agtgctcggg atgcgggaga acgggttcat cgcgagtttc atcgaggggt gagcgaagag
      841 tggagcccgg actcccgctg aagctaggcg tatgaagttc catattcgca cggtggtggc
      901 tcccttcacc attattcggc ttgttccctc attttatctc attttgcaag accttgcccc
      961 acctcccctc ccctaacttt ttcctctccc atcctccaaa ccgtggtcgg ggtgacggag
     1021 aatctcacgt gccggcgcac ctccctccga aacgtttgtc cggctgaagc tggaccgttg
     1081 ttaccatagc ccaccatagg gcggtatggg ccataaagac tacttcctgc tgttccgttc
     1141 gatggaggaa ggaagacggt gaagagaggg agatccgggt agtatgcctg atgccgggtg
     1201 tatgttgcca gtgtaaaata caggcggaag gctccgtttt tctctcccgg cgtgcgcagg
     1261 ggtctcctta ccccgccccc ttcaccctcg caagaacaaa tcaatgagtt cttgtttagt
     1321 catattgtta tggtttgcga tatcgtttaa gatattgttc actgtgactt ccgcgctggt
     1381 ggacaaaaga atatccaagc tttttgagaa ccctgatgat atctgtgctg ctaacaactg
     1441 gaagatgtgg caacgtgatg cacctgatgt gtaatctggg tctggatcgt gatttgttcg
     1501 ttgggttcca cttcgtcttc gaaatacagc agtacagctt cttcaatgtt tttaagcaat
     1561 ttttcgaatg tttcaccatc ggtaaagata ctgtatccga ttgcgctggc agaccaatat
     1621 ccgtcttcat aggtagtttc aaatcgtact atcatgatgt aatcccccga gttagagaga
     1681 atgaaacgtg aatgatgaaa aatgttgtta cgaacatcaa acggagatca gagtggaaac
     1741 gatcggtagt gcgccaggca attactgata tcaccttcac aaacctgact ttccggattt
     1801 ataacttaca ctgactcaat ctcttctcgg ggaagagatc ctgaaacccc atcgcattca
     1861 gttgatccct catcttatcc gtcaggttga ggtaccagaa cgctttctcc tgccccgcac
     1921 atcccacctc aacatactcg acccgtttca accgccgcaa cagttcatgc cggggggatg
     1981 gctaccatga tattatcgta tgttgcggag gtaccagtgc cggctctacc ggaatgccga
     2041 tctgaccctc agagtccggg agtggatcta ttcctaatac gggatgcatc acgaccggga
     2101 cctgaacgcc gcgttcgatg tcaaaccgtt tgcacgtgca ggcgtggaac gtgccgaaga
     2161 tcctgtgggt tcgctcccaa tggggagagg acgacggccg ggaagccccc tgcgaaagcc
     2221 ggggggtagt tcacagcacg acgatgctcc ggcaccgaga tctcgttgtt ggcggggtag
     2281 ttcacgctcc cctcgaagat gggcgaagtc gctatcgcca ccgcatcgcc catgatcgcg
     2341 gtatgcggag tgcctatcgg tcttgcaggt atcggcaccc tcgctctcat cggcaccgtt
     2401 atctccgtct tgctcgcggc agccggactc acccggcagc gggtccgcag ccggaaaggg
     2461 tgagcctcac cgctggcgac gtgccgggcc ccgcgcgcag tagaggtact cctgtgcata
     2521 cccggcatac tccccgaagt agtcgcggct aaatcgccgg atccgttcgt actccgccgg
     2581 ggtgcaactc cggcctgcaa ggtcggagag gtaggtctcc cccacgatcc ggcgtatcca
     2641 gacgtcgacc gggaacgcct caaagaaccc gaacgcaaag aggagcacgc agtccgccgc
     2701 cttcggcccg acgcctttaa actccatcag cgcccgccgc gcatcctcga agggaagggc
     2761 cgcgattcgc tccgcccagc cggggttctc gcttgcgaag cgggcggcct cgcagatata
     2821 atccgtcctg taaccaagtt tgcagtccca tatatccgtg cgtgaagcct ctgcgagcgc
     2881 ctccggctcc ggaaacgcgt acgtcatgcc ggacggcccg tcggtcggtc tcccgtagcg
     2941 ctctgccatc agggcgaccc gccgcttcac cgccgggatg ttcgtgttcg tggcgcagat
     3001 gtaggaggcc aggcactccc acggctgctg ccggacgagg cggaggccgc ggcactcccg
     3061 gacggcggcg cctataaccg ggtcgcggtc gatcgatgcg agaatggcgg gaaggtcctg
     3121 gtcgagccgg aggtagtcgc ggacgaacgc cgcatccgcc ccctcaaacg tgagccggtc
     3181 tgcgtcctgc cgtatccgga cggcccgacc gtcgacgacg ccctgccacc acccgtccac
     3241 attctcccag cggaaagcct gcccgcagga gagcgtcagg tcgaggtcga gcggctggtc
     3301 gggccggagt tcgatcgtcc tcatggccgg ccgaaaaaaa gttggttatt ctgccttctc
     3361 atccttcatc tgctggtaga cgcaggactg cacgtcctgg cctaacttcc cgatcgcatc
     3421 cgcccggcag cgtgcgcagt ggcgcatctg cttgatgaac ggggcgcacc ggtcctgcat
     3481 ctcccgctta tcctttgccg tcgggggggt gatgtcggcg aacttatact gcgggatgag
     3541 cggtatgacg ttgaaggtga acgctcccat ctcgccgact ttctttgcga tcgcggggat
     3601 atgctcgtcg ttgatcccgg gtatgtagac ggtgttgatc ttgacgaaca tcttctttgc
     3661 gaccgccatc tcgatcccct tcaactgctg ggagaggagg agctccgcag cctcgcgccc
     3721 gcgatacttc ttccccttat actcgaccca ggagtagatc ttctctccga tcgccggatc
     3781 gatggcgctg agcgtcaccg tcacgttccc gacgtcgtac ttcgccagtt catcgatcga
     3841 ctcggggagc atgaggccgt tcgtgctgag gcacatgatc aggtgcggga actcctcgtg
     3901 aaccagcctg agcgcttcga acgtctcggg gtttgcaagc ggttcgcccg gtcccgcgat
     3961 gccgatcacc ttcacaaacg gatagtcctt gacgaccttc ctgaccaggt cgaggccttc
     4021 ctccggtgtg agaatcttgc tcgtcacgcc cggccggctc tcgttcacgc agtcgaagtc
     4081 ccgcacacag tagttgcact ggatgttgca cttcggggcg acggggaggt ggcaccgtcc
     4141 aaaggcgtgg catgctttgt cggagtagca ggggtgctcg ctgatcttgc ggagctgctc
     4201 cggatcgtag gggacctctc taccctggac cgttgcggtc cggtacgatt catcggccat
     4261 aatttatgat ctatgcgccg gaggatatta tatactgatg ggtaccggtc gcctgcagcg
     4321 agatcggcgt gggataggaa agacgaccgg gaggatgcag tggcctgcat tctcccggaa
     4381 aaggtaatca ggcctcctgc cgggagaggt cgagcggaac ggtctttgcc tccgcctccc
     4441 gcgcacctgt ctcctggtcc atgccgaacc gctcttctgc tgccttcagg acccgcccga
     4501 gcatatcggg ataagagagc cctgcatact tcgccatctt tgcaaggtgg ccgtcccagc
     4561 accagccggg gttcgggttg acctcgagaa gtttcgggtt gccgtgctcg tccaggcgcc
     4621 agtcgaaccg gcagtagtcg cggcactcca ggcgctcgaa gagggcaagg cagcagcgca
     4681 cgaccacctt ctcggtctcc tcggggaggt ttgccggccg cgatacgatc ttccagtacg
     4741 gcgagtcggg gagccacttt gcctcgtagc cgcagatccg ggggagttcc ggaggcaggg
     4801 cggagtagtc ctcctcggtg atggggagca ccgtgtagta gcccgacggg ttcccgatga
     4861 tgccgacgct gatgtctttg cccgtgagga actcctcgac gaggagcgac tggctgtacc
     4921 cgagcgtcgt ccgcagggag ctgatgatat cggagagttc ctcgatggtg tgggcgatgc
     4981 tcctctgcgt gatgccgtag gaggagtcgc ctgagttcgg cttgacgatc accgggaact
     5041 tcatcccgac atcgtaggtg cggtcgcccg gggtgatgaa gcaggcatcg ggcaccggga
     5101 tccccatctc ctttgcgatg ccgcgcacga gcgatttatc gtagcagaac gcaagcgact
     5161 gcggcgcgga tcccgtgtag ggtatgtcga gcatctccag gagagccggc acgtggagct
     5221 cttttcgtgg gtcgttgttg aacccctcgt cgcagaggtt gaatatgtag tccaccttac
     5281 ccttcagttt cgtgaggtcc gggatcatgg tgtcgtgttt gcagaggtag gtgaaccggt
     5341 aacctttgag ttcccggagc gccgccttca tctggtcgat ggtgtagaaa tcgtcgtcgt
     5401 caaagacgca ggacggtttc agggcgtcgg ccttggtcgg gtcgccgagc atcaccgcga
     5461 catgttttgc cctctcctgc cccttcgcct ttgccttcgc ccactctttc ctgattgctg
     5521 cggtgacgat gtggcgccgc tccatcatgc cgaggtcctg gtttcgctgt gattccgttg
     5581 cgatctcgtg gaaggcgata cgggagaacc cggcccgttc caggagttgt cggagcgcat
     5641 cgcgggtgta gaggcgttcg gcgtagaact ggtcggccac gacaccctta tcgacgtcgg
     5701 tgatcacctc ccgtgagatc aggcgctggc cgtcgagcga gagggaccgt tcacggcaga
     5761 cgaacatcgt gcgatcgatc cactcccacg accggggctg aaagtgctct ttgaggtatt
     5821 cgccgtcggc cacatccagg agcacctgcc cccagggttt gagtatgcgc cggagctcgg
     5881 tgaggatccg caggtcttcc tcgacggagt cgaagtagcc gaagctgttc ccgaggacga
     5941 ggacgacgtc gtaggagtcg gtccgatacg gcaaccggcg tgcatccccc tcccggaacc
     6001 tgacctgcaa cccctcccgc ttcgcggtcg accgggctcg ctggatcagg taatgcgact
     6061 ggtcgaggcc ttcggcgttg tagcccctgc gtgcgagttc gagggtatgc cggccctggc
     6121 cgcagcagag atcgaggatc ttctcgtcgg ggccgaggcg cagaacccgg gcgacccggt
     6181 cgatctccct cccggtgatt ctctggtcgt cgacgacgtc gccatcggtc ttcaggtaga
     6241 ggtgattgaa gatacgcctc caccaatcgg attttacgtg ctcttcaagg ttcgggacag
     6301 gaccgatcgt ccgtttatcc cgggttctcc ccttccgcct gctatcaggg ggttcgtccg
     6361 tgtggatctc gtcggtcatg ctgagccact catcctggtt ccgtctccgg aacagattcg
     6421 gcatttcatc cgattacaaa aaagattgat tgcgtgtacc atagctgggt gatcattcgg
     6481 taggacggta ttgataaaag cattgtgcca tgagtctctc taccggaatg aactgccgca
     6541 ttatcgggga tttctatgcc tcgaaagttt cgccccacgc gccgggacag cggagcgggg
     6601 tataccgggg ggtgacggaa cccgccggat tccacgaaac gcacttatat atggcggcag
     6661 gtagttgtac atatgcagac tatctgcatg gttgaagtat atggatgaga gatattcagg
     6721 caggggcccc cgctcgttcc aggaccgccc ccgcgagttc cataaggccg tctgttccga
     6781 ctgcggcaaa gcgtgcgaag ttcctttcaa gccgaccgag ggcagacccg tctattgccg
     6841 cgactgcctc ccgaagcaca gaaaaccccg gtattgagtc gtttagtgag tacggtttct
     6901 caacgtgagt gaaattccaa tggaagagag atatccgagc cggggccccc gctcgttcca
     6961 ggaccgcccc cgcgagttcc ataaggccgt ctgttccgac tgcggcaaag cgtgcgaagt
     7021 tcctttcaag ccgaccgagg gcagacccgt ctactgccgc gactgcctcc cgaagcacag
     7081 aaaacccaga ttctaactct ttttttgtgc ctgcataccc gatacagggc gggttgcccg
     7141 gaccctcccc ccgtcgtgcc ggctttatat gagtcccata cgcccctgag aacccgggtg
     7201 aaccggaacc cgcccgtcac acgatcagcc gtctcctcat cagcctgatg gcgaggacga
     7261 agaagaacgc ggtggcgacc gcgatccagg cgaggctgat gaggtttgcc ggcgcgaatg
     7321 ccgggagggt gatcgcccgg ttgatgctga cgacgtgcgt gagcgggagc acggcaaggg
     7381 cgaagtaccg gataggttcg gggagcaggt cgaggggaaa aaacgtcccc gagaagagga
     7441 acatcggcgt gatgaagagg aacgcaggat agttcagcgc atcgatgccc ggcgtgatcg
     7501 ccgtgaagca catcccgatc gttgcgaaga ggaggccggc aaggtatgca aaggggatga
     7561 ggaggaggga cgagggcatg gccacgacgc cgaagaggag gagcaccggg agcatcagcg
     7621 ccgcgtagat cataccccgc gtggcgcccc agaacatctc ccccgcgatc acctcatcga
     7681 tgctgaccgg tgtcgcgatg atggcgtcaa acgtcttctg gtagtacatc cgtacgaacg
     7741 acgagtaggt gcactcgaag aaggacgagt acatcaccga tatcgagacg agcgcgggcg
     7801 ctatgaagac ggggtagggt atcccgtcga cggactcgat gtacgtcccg aggccgaacc
     7861 ccagggccag gaggtagagg accggctcga cgaagggcgg gatgaagttc acccggtagg
     7921 ttttgaggaa ggcgtcccgg ttcctccgcc agacgctcca taccctgccg gtgatatcga
     7981 tcatcctctc ctcactcccg caggctccgg ccggtcaacc ggaggaagac atcttcgagg
     8041 gttgccgggc gaaccgtgac cgcctcgtgc cggcaggtct cgagcagttc gcgggccaca
     8101 tcgtgagggt gatctgtgaa gacctggatt gcaccgccgg caaggtcgta gtcgagaccg
     8161 aactcatcga ggcaggtaag caccgccggt gtccgttccg cttcgacgac gttgctcccg
     8221 gcgtgctctc ggatgaggtc cgccggggca ccctcgacga ggatcttagc gtgatccatg
     8281 atcaccagac ggtcgcagag acgttccgct tcgtccaggt agtgcgtggt gaggacgagg
     8341 gtgtttcctt ccgcccggag actcctgagt ttctcccaga tcaggtgtcg ggcctgcggg
     8401 tcaagcccga cggtgggttc gtcgagtatg agcagttcgg gtgcattgat gagggcccgg
     8461 gcgacgatca accgccgtct catgccgccc gagagtctat cgatcagcac gtcccgcttc
     8521 tcctcgagtt gcatgaacgt aaggagctct tctgcccgcc gttccgcctc ccgtttcggt
     8581 atgccgaagt aacgggcgta gatcagcagg ttttggtagg ccgagaagtc cgggtcgagg
     8641 ttgttctcct gcggcaccac gccgaggcgg cccttgatct cccgctggcg ggtatccaca
     8701 tccatgccga ggacctcaag ggtgccgccg gtcttagggg agatgcactg gatcatcttc
     8761 atggtcgtcg tcttccccgc cccgttcggc ccgagaaacc caaagacttc tccttccctg
     8821 acccggaagt tgatgcggtc gactgcggtg agatccggaa accgtttctc gagatcgcgc
     8881 gctacgatga cgtcgccgat tatgtgctcc tcctgatgag tgagtggggt cgtgcagata
     8941 gaagatcctt tcgcttgctc gtccccctcc ctctccgggg gggcaggaga gatcccgcgc
     9001 gacggatccc cggccggcta tatgccggat ttttgcagga aaaccgggtc ccggttcggt
     9061 ctatccgtat tcgatcccgc attctttttg agcaggtaga gccaaccact gtaattcata
     9121 cgtttctgcg gttcccccgg ggagcacacc gtctatcacc cctccacgca actccggcgc
     9181 gccggagcga ggcggttcac aggggtgagt gccccggggg aggtgcggga ctccagtgat
     9241 accatggcag aatgtgctga agagaaaggt gcgaggatgg acagggccat atacccttct
     9301 gatatcgtat acgtcggttc gacggagaag cggggccggg gagtatttgc ccgcagggat
     9361 atctcggccg gtgagttgat cgaggtctgc ccggtgatcg cgatggatga gacggacggg
     9421 cagaggctcc tcgatcggac gggcctcttt aactactact tcgcctgggg gcggcacgaa
     9481 gagtcggcag ccgtggcgct gggctacggg tcgctctaca accactccta ccacgcgaat
     9541 gcggatcacg tccgcgacgt tgccggcggc gagatccgca tctacgcctg ccgggcaatc
     9601 cgggggggcg aagagatcac catcaactac ggtggcctgc ccgactgccc cgaccccgtc
     9661 tggttcgatg tggtggaaga ggagcgataa ctcctcctct ttcccgcaga tttctctcca
     9721 actcacgccg gtcgtctcca tccggctatg gtgcctgcgg tgccccggcg gggtggttga
     9781 catccctatc ggcgcgatgc cgggagcccc ggctatctgc tctccgggta tctcccggcc
     9841 gcgtttatac cccggtgacg tgcggcgtct gccgatagta cgggatgaga cggcaccccg
     9901 gcccccccgg gccgccctgg tggttatgcc taaatctcca gtggaaatca aggggtttag
     9961 cgaaataact caggatcatt gaattctgct cccgcctcca taccgtctgc cggtccctgt
    10021 gatcgtttct caagccgtat gggtcgcccg gaagagctcc atgatttgag gcattttggg
    10081 gaattcacgg gcattttcga cttatctagg tagggtatag gggaagcgga tacatgcggg
    10141 cgggagcgtg atcaggagac gccgggatcg gcctgcaggg gccgggggtc ctctaccagg
    10201 tggccgacag tgcataacgc ccgccgcaac tggtaataaa gggcgccatc cggggtgggg
    10261 cggtggccct cgccccgctc cgggagggag ggcatcccgg ggtacgaggc actcgagcgc
    10321 cgcccgcgta ccccgttctc aggtcccctt tgccgcccgg agggttgttg cctctccgtg
    10381 acggccgggg attctacttt gtcttttcgg ccagggaccg gtcatacctt cgcgccttct
    10441 catcgccccg cgtgcagtag aatgccgtat gctgaagtcc ggtatccttc ggaacctggc
    10501 acgtagggca gatgcactcg acctctcggt cgatgcagat gatgctcatg ccggtgaaac
    10561 agaagaagcc ctggtccagt ttctccgcac attcggtgta ggtcgggcag ttcatgcaga
    10621 tgcacttcgc tcgctcggct ttgagggcat ctatgcgcgc gataaggggc atcttcatca
    10681 gtttctgtgc aaattgttcg aaagcgtcca cgtcgtcact ctcctgatct tcgcgccacc
    10741 tgttccatac aggtataaaa gaccgcctgc gtgaatacag gggtcgcggc agggtgcttc
    10801 tcgtggagca taccatgagg taaaacagtc tggcgccctt ccggttgctc cggaagggca
    10861 gatctcggta cggaggcgac cctgatgggt tccggccggt cagtcgatgg cgaccatgac
    10921 ctctgtcgac ttgaccacgg cgtagacctt cttgccgaca gcgagcccga ggttctccac
    10981 ggccttcttg gtgatgaccg aggtgatctc accgccgccg tccagtgcga tggtgacttc
    11041 ggcggtgacc acgcccgtat cgatcttcgt tacttttccc gggagctggt ttcttgcact
    11101 taacttcatg atgcaccatg atgctgctct acgccctcag tgatatacgt tgcgatctcc
    11161 ggaacccccg tgcggttgtg tgccatagaa agcggatatc ccgtgggcga acaggcaaat
    11221 cgacagcagg aatgagtgcc ggctataccg gggctcgata tcgctcttgc tgcggataag
    11281 ccggggctga agttcgagtc gggttcgccc cggccgggcc ggctgaccca gaagccatgc
    11341 gcgtacaaac gaggggatcg ctatcccggc gactggaccc ccgcctattc catcagtccc
    11401 cgatctcttt ttcccgctcg ttccggcacc tttaaccttg caggtgatct ttatcctgat
    11461 atgagcacga tgagacctct gcgcatcgct atccttctct tctgtttgat cctgaccgtt
    11521 cctgccgcca ccgccggaaa cctcaccatt ctgcagcctt ccgcgaacga gagcacgttt
    11581 gccgagatgc gggatttcta tgtctacggg gtctttccag aaccgctgga acacccggga
    11641 aacatccgcc tcgtcctcac ggatgagagc ggcgtgatcg tccggacggt gatgagctgc
    11701 gtcaacgaga ccggtgtcac gccggaggcc gcggtggaga tgagccttgt tccgaacagg
    11761 tggggagggc tgcttgctcc cgacgtcatc accgaacccg ggggcatcgc gaacggcagc
    11821 aacaaggtgc tggtgaccgg gacatactac cttgccctgg tgcagggcgg tgtcacccgg
    11881 gggatgggga gttataccga tgcgagcggc ggaatgctcg cgccgatcac gcgggacctg
    11941 accgcaggga cctacacgct ggtcgttgag gggctggacg gggatctcgc cggggagagg
    12001 gtggaaaaag agctcgtctt cggcacgacc catgcctctc ttggcacgtt ccgccccgat
    12061 gcgaacaggg agaacgtcgt cggctgggct cgggagaggg tgccgccgct ccgtgtctat
    12121 atcgactggt ttccgggata cttccgatcc ggcgacgcag gatacgagat cccggaccgg
    12181 tggcaggcga acaacggtat cgaggtagca aacgattgta cgggaacaac ggtcgacacg
    12241 atcgcctctg cacgaaacga ctgcctcatc tacaacgtcg gcagcaccag tgcgaccctg
    12301 caggtggaga ttgccgcgat actcaggaac ggccttgtgg actccgatcg cacgacgttc
    12361 acgcactacg acatcggcga accgtcgatg gcatgggtcg accgggcaac cggcgcggcc
    12421 gaatcgatga tcggaaggca ggtggcgtat cccgccggca cccggctggt ctacacccgc
    12481 gcggagatca cggatgaacc ggtgcgcacg aattacttca acacggtggc aaacgatagc
    12541 agcaagaccg tcgatacgac gccgatgcag gtgacggtcg tgcagggaga gaatttaacg
    12601 ttctacgggg tgacacggcc gattgaaacg acgctgacgg gtgccgggag cgcctatcgg
    12661 tacacccccg acgaccggat tgcaacgtat atctacgaaa acgagacgct ggggcggttc
    12721 acgttcaccg ggtgcctgga acgcgagttc gagggcggct acatcggtgc tgccggctac
    12781 gagttcgggc atgcgttcac agagaccgcg gcgatgccgg caggaacctg gctgttcgcg
    12841 gtgagcgcct acgacacgac cggcacccct gtggagatga cgaaccggac gatcgagctg
    12901 gaggtagcgg caccgcccgc tctgcccacg gcgacgctcg ttgcagcggc ggcgcctgcc
    12961 ccgacccgga cgtccgggtt ctggtctgtc ccggcgctcg tggcgcttct ggcggcggtt
    13021 gcggctcgcc gcaggtgagg ccggacaggc agccgcctca cccggaactc ccctgcagcc
    13081 ccgatacgca atggagggca taaggggctg cagcgcggcc gggtgcaggg aagaacccat
    13141 gcctgtacgc cgatgatgga acgagagagc aggtatggtg aacgatcaaa cgagaggtag
    13201 cagcgacgtt tatcgccgac cgtgagcatc tgatggccac gatcacagag gcatttaaga
    13261 agggagcggc gaaaagcagt tatcgggcga aatgaacggg tgaattcttg agtccgaagc
    13321 atgcttccaa atcgttacgg gagtgactat aagcaagaat agccggccct caataggcgc
    13381 gcagcgcgaa cgtgattctc cccaaaacta tatctttcca gatatagaca tcatctgtat
    13441 gccgacgaag acggtagtcg agcggaagaa ggcaaccccg aaggccgcgc ctaaaaaggc
    13501 ccttaccccg gccgagcagg ctgaacggca ggccagcatc gatagattag atgcggatat
    13561 acgaaagagc aaaatgggta agagttatac tctggccgag ataaaaaagg aattcgggct
    13621 ataaatcgat gcgtttatac ctcttgtgcg ccgccgcgta actcataatc aggtgaactt
    13681 cgacaaacgt tttttccttt gttctctcta ccgtgtaaaa tatcgtgtat ctcttctgaa
    13741 catgcaatcg atgggccttc tcctttcctt ccatcttctt cttactgcat tggccgattc
    13801 gatcgaaaag gtgctcgccc aggcagaact ctattttttc gaggattttt gacacatact
    13861 caccatcatc gcgctcgatt accttaaggt ctttctttgc gctctctttg aatacgatct
    13921 cgtacttcgt catgcgcctc cctcagacct cgacgccccg cgcacgcagc gcggcgatca
    13981 caccctgttc tgcagcttca gacataggga tgctcgcttc cttcgcaagg aggtaggcgt
    14041 tctcacgggt atagatggag gtctgctgaa cgacctcccc ctttgcgttg atgcgcttca
    14101 tagtcagtac gtggctattg tggcctctta aagagagggt gtcaaacaaa ccgccctttt
    14161 ggcacccgct ctttatggtc cgcccgcaca gagggtaaag catcctgagg ttatgcctgg
    14221 gcaggagaaa aagaaatgag tagatggagt aaaacggcag aatgcccgtt accagaacat
    14281 gtcaggaagc ggttagaggt cactatcgac cctgagaatc ccgagtgtcc aatgaaatgc
    14341 gccattaacg tctcttgatt gctcgataaa gcaatctccg ggctcagaaa cataacacag
    14401 cacagtttag cccttgtttc gaaaaaagga aggagagaaa tgggcctgcc cggattcgaa
    14461 ccggggatct tcgccgtgta agggcgacgt cataaccgac tagaccacaa gccccgtgga
    14521 tgccttacag tgtttcgcac cgtgagggat aaacgttgtt gcctcccgga ggctcccggg
    14581 catgggatgc cccccgaaac gaggttcccc cggcggggtg tcgcctccgc tgcccgcacg
    14641 tcgactctgc cggccatccc gctgcggccc cctccgggcg ctgccgaaac gcccgggtcg
    14701 gtcaggaaaa gggaggagag atgagttcag atgaacccga aagatttgag cgtcacgtcg
    14761 gggttgacgg cccccacgtg atagacggcc ccctcggaga gctcgttgat gacgacctcg
    14821 gcgggggaga agagcgatgt atcgatctgg tagaagtcga acttggcttc cttgaagatc
    14881 tcatagaacg gttttccgta tccccgggac ttgttgctcg ggatcctgtc aagataatcc
    14941 ttgatctccg gggcgttcat cgtcagcatg atgctgccgt ggtagatcgt ggcgtcgttg
    15001 gtgctgccca tcgccctcgt gccgtccttc ttgacggggg cgatcggggc atggccgata
    15061 ccggcggtga tcttcctcgt atcaaaaccg agctcgttca gtttgtagac tgccgtctcg
    15121 acgcaacggc cggcaacctg gaccgagccg acgatcgatg ccgtgggggc gacaaccgca
    15181 caggtgtttg ccacgtccac gtcgcaggcg tcggcgatct tctccatcac gccggcattc
    15241 gggagatggt cgctctcgag ggcgatgacc gcataatcga actcgtcctc gtagtcgatg
    15301 acttcgtaag tgtgcttcgg ctttaaggag agcgctcgtg ccggaccgct gcccatggcg
    15361 aagtacttgt tgacgttgac cgtccagcct gctttctggg cgccgaggca tgcgatggcc
    15421 gggaagtcgg tgctgacctc gatgaacggg atcgggacat ccctgatccg gcccatcgtt
    15481 atgtcgacct cgccgagtcc gcccatacag atctcggtga accgtcttcc tgccaggtac
    15541 ccgccggtag tgctgacgcc gcagtctacg atgcgtgccc cgttgtcaaa ctcgtgcggg
    15601 acggcatgga actcttcggc gtattcgaag agttcctcaa aaatatccag tgccagttcg
    15661 tttacgctga gcatgtggaa aacctcatca gaatacaaac ggtggcagaa cagataagga
    15721 ttatcaaatg ctacctgtct gctcccgccg caggtgctca agcaccctat ccggatcgta
    15781 acggaggctg atcagccgcg tccgccccct tccctgccgg taatggaggt ttaagagccg
    15841 catcgcatcg aacttctgga tcatcttgta gtaccgggta tagctgatcc ccacatgctc
    15901 cctgacgaag tgatagacct ctccggcgtt cgtctcccgg tcgtcacgaa tcgccatctc
    15961 ggcgatcctg ccgagcacct cccgctcctc ggccctgagc gcccggagag agtacgcaag
    16021 gtgcaggtac cgggagacct tgtaagcctt gcagatatcc tcctgctcga tggaccggcg
    16081 ggccgctttc tcggcgctca gggtcgcccg cttcagaagg tcgatgccga cgcgaaggtc
    16141 gccgctcttc gtcgtttgct ctaccacgag atccagcaga tcggaccgga ggacgttcgg
    16201 gtagagcccc tgcacgaccc gctccccaag gataccccgg atctcctcct ccgaataggg
    16261 cgggaagtat atctcggtgg gccggaagac cgacgccacg cgggcatcca cttcggtctg
    16321 caggctgacg gacatatcgc tgacgatggc gacgacaccg atccggacgc cgggatatgc
    16381 ctcgtgagaa cggagcaggg agtaaaggac gtggttgatc tcgttctcat agagaaggta
    16441 gtttgcgtcg tccagtccca ccaggagcac ctgctcctcg cgctggagag cccgggctat
    16501 ggcatccagg agctgtttga acgatacgcc tgatgccggc ggcaggtgcc cgaagatccg
    16561 ccggtagatc tgcgaaaaga cagcgaattt ggtgttgtcg atctggcagt tgatatagat
    16621 gggaaccagt ttctgcgtga cctcctcgat ctcggcaaac accttcctga cgctcgtcgt
    16681 cttccctgtc ccgggaagac cccggcagat ggtgttgagg ggccgccctc cccggaggcc
    16741 gggccggacc tgaaaggcga gctcccggat ctgggtgtca cggtgattga actgctcggg
    16801 aacgtagtcg atctcgaaga cctcagggtc ccgaaagagc gtctcatccc acatgagcag
    16861 gctcctcttc atgatacaat cctcgtgtgt tgtagtattt atatcctcca ggtttcagtc
    16921 tcctcatcgc gcgggtccgg caggcgttat gggtgttcca tgcacttctt gcagagcttc
    16981 ttgctcatga agagctggga gagtttcgcc tgcgatttcg tcacttccgc tccgcagatc
    17041 tcacagacgt catccgccgg ggccccggga ggctttgccg tgggagtctc ctgttgcggt
    17101 gcctgcaccc ggggcgtctc cggttgaacg gaaaccttct tttccggtcg agcgggcggt
    17161 tcgaggaccc ggggcgtctc cggttgaacg gaaaccttct tttccggtcg agcgggcggt
    17221 tcgaggaccc ggg
//
LOCUS       DAOSEE010000033        17233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1351_length_17233_cov_220.639714, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000033 DAOSEE010000000
VERSION     DAOSEE010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 17233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17233
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1351_length_17233_cov_220.639714"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1183)
                     /locus_tag="PLI76_04045"
     CDS             complement(<1..1183)
                     /locus_tag="PLI76_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOI61235.1"
                     /translation="MAATIAEKIFSMKCGRSVRAGDVVMAPLDAAMIHDITGPLAVRV
                     FGEMGGERVFDPERIIMLFDHQVPADSIPAAENHVFMRRFAEEQGIHNYDLLEGVCHQ
                     VVMEKGRAAPGEIIVGTDSHTCTYGAAGAFATGIGSTDMGFVLKFGALYFRVPESILL
                     EVDGVFDRRVGAKDLILSLAGDIGADGATYMTLEFTGKAMERMNMAGRMTCANMAIEM
                     GAKAGIVRPDAATWEYVAARREIEPFDLAGDDDAVYRERRHYDVTDLAPQVAVPHNVD
                     NVVDVADVAGTKVDQVFIGSCTNGRYEDLAEAGEVMGNADRFAKSVRVIVIPASRTEY
                     LKALRAGLIERFVEAGALVEAPCCGPCMGGAFGLLAPGEVSLSTSNRNFRGRQGSAQA
                     LV"
     gene            1330..3948
                     /gene="mutS"
                     /locus_tag="PLI76_04050"
     CDS             1330..3948
                     /gene="mutS"
                     /locus_tag="PLI76_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOI61236.1"
                     /translation="MTDGPTPAMRQYYSVKNRYPDAIIFFRMGDFYETFGEDAGVVAR
                     ELDITLTARGKDRKGDRMPLAGVPHHAADGYIARLVGRGYKVVICDQVEDPKTAKGVV
                     KREVTRVITPGTLIDSSMLGSAGARYLMAVAPDRKDTFGLAFLDVSTGEFFVSAGSGG
                     REYADVVSEAVRYRPSEAILPEALDEGLAGRLESIGVTVSRYRDDAFDPDAACRLLCE
                     QFGTATLDGYGCAQMTGAVAAAGAALHYARETQQSPLPHITGLSTRVPSGTMVLDAIT
                     LRNLEITTGIRGEGDRSTLLAALDVTETSMGSRTMRSFLVAPLVRKAAIEERLDAVEW
                     LIGHTVERQALRAALGDFADIERIAGRIAYGNAGPRDLAMLRESLSAVPGVKALLPGD
                     LPTLVREALDAMSDHASASDLIGRAIVDDPPALAKSGGMIREGFNAKLDELRGLATTG
                     KDWIVEFQQQERERTGIRSLKVAYNRVFGYYIEVTKPNLPLVPPEYERRQTTANGERY
                     TIPDLREKEALIATAEERLSALEAEIYAELVRTLAAEVPGLQATARAVGLLDVYSALA
                     EVAARYGYSRPAIEESGRIVIRDGRHPVVERHLPVPFVPNDTELDSAGDQIMIITGAN
                     MAGKSTYMRAVALCCIMAQMGSFVPARHATIGIVDRVFTRVGAFDDLASGQSTFMVEM
                     LELANILNNATPQSLVVLDEIGRGTSTLDGCAIARSVVEFLHGRAIAGPRTLFATHFH
                     DLIDLEGSQKRVKNFHFAVKDTGQDVVFLRKIIPGATDRSYGVHVARLAGLPKKVTDR
                     AMDLLKEASVRELPTGVRAPRYTQMLIVDPGEGAVAENPAVGELKRLNPDEMTPIEAL
                     NALCRLRRMANGEGER"
     gene            3945..5690
                     /gene="mutL"
                     /locus_tag="PLI76_04055"
     CDS             3945..5690
                     /gene="mutL"
                     /locus_tag="PLI76_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOI61237.1"
                     /translation="MTKIRILDPDTVNQIAAGEVVERPASVAKELLENAIDAGATSIL
                     LDVGSDMAGVTKIRVTDDGAGMTAKEALLAFHPHATSKIQDIADLSTVRTLGFRGEAL
                     ASIAAVAEVTLVTRPRGAGALAGTRVVVRGGEVVETGDVGAPEGTTIAVEHLFYNTPA
                     RRKFLKSRNTELAHLYGVVENLSLAHRGIAFRVVHNGKERMAVQRSGELIATIAGLYG
                     ADLARALVPVGGRLPFLSIGGYVSRPSESRGNPSQVVISVNGRSISSRPIAAAVREGY
                     GTLLPKDRYPVAFIDLAIDTDLVDVNVHPTKREVRLSREREILAAVAAAVEEALLGHD
                     LAGATPAEPVQQVLTGERPEAAPAAREAEAAYAAGHRDIARSDRQLRRTEGEGEENLL
                     PAMEPVGQVAATYIVAEGVDGTLYLIDQHAAHERILYDQVAERRDAAPGSQELIMPVV
                     LSLPPKESAALRDAMPFLAEEGFEVEEFGRDTFAVRAVPAALGAMEDPESIRETIADI
                     LAGESRTTPGRREAVTCIVACRGAVKAGTLLTHDQQRRLLSQLARTKTPWTCPHGRPT
                     VVAFDRRRLDGMFGR"
     gene            5862..6026
                     /locus_tag="PLI76_04060"
     CDS             5862..6026
                     /locus_tag="PLI76_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61238.1"
                     /translation="MTLLQQIRLWMQTRGEGKQLHDCENDAVIEPYFDLSPEATLPPE
                     TRENLLEEDR"
     gene            complement(6140..6733)
                     /locus_tag="PLI76_04065"
     CDS             complement(6140..6733)
                     /locus_tag="PLI76_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61239.1"
                     /translation="MKPQSLLIIGVLAVALVAVLAILPPGPAAPQPPGPGEGEPAPAP
                     TPAPLPENGTYINTTYGYAVTCPAGWFFTESGESVWFSSPVKHEEVRINTIPLPDGNS
                     SREAILETLNATYAEDLGTDIGAVWVSTEKTALDGIPAYRSVYSVPIVESDRYTFIIR
                     YAVRDDIVYSVMHTVFPEGYDSYNADAAAVAESFEFI"
     gene            complement(6799..7632)
                     /locus_tag="PLI76_04070"
     CDS             complement(6799..7632)
                     /locus_tag="PLI76_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S16"
                     /protein_id="HOI61240.1"
                     /translation="MRVREKTLTILFVLSLFANVFLLAVALVPGDDIPALFPPDAGPS
                     PAATMASLPGIGRTASMQAPVTLQKVEITGIGPFSTNRMIEEGAMVNISVEIVPGRGR
                     VLVQTTPLMGIVFQDAANRAVDLAAGRSHADLAGSDIIFSIQSPDGVSEIDGPSAGAL
                     MTALLLSVMEDFALNESVTVTGTIDENGNIGPVGGILEKAGAAAASGKTLLLLPAENN
                     NIVESREETGIFCGLRIARQRPVAVDTGMYIEERYGIRVKYVHTLDDFLADVCTPAAT
                     P"
     gene            complement(7707..8777)
                     /gene="egtD"
                     /locus_tag="PLI76_04075"
     CDS             complement(7707..8777)
                     /gene="egtD"
                     /locus_tag="PLI76_04075"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HOI61241.1"
                     /translation="MTGRLSTPSVRSELRNYLISAYRDDLASDILSGMTAEQKYIPSR
                     YFYDARGSQLFEEICRLPEYYPTRLELSILCGACGALMESFGEGDLVELGSGANKKIS
                     LLIDAAPGPEGEGLRYIPVDVSESALAAATEGLLWGYPHLHVLGIVGDFTRDIERIPR
                     GRKRLITFFGGTVGNFTGPEAHRVLVNVAAMMEEDDRFLVGLDMVKPVPVLEAAYNDT
                     AGVTAAFNRNILAVVNRELGADFDPGLFEHRAFYNRDLAQIEMHLVAREAMTVRIPAL
                     GLRVAFERGETILTEISRKFTRESAGRLFGAAGLAVERWFSDPRGWFALASLSRAAGR
                     PVEPAGMAQDPPARGGPAGIHR"
     gene            complement(8844..12236)
                     /locus_tag="PLI76_04080"
     CDS             complement(8844..12236)
                     /locus_tag="PLI76_04080"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOI61242.1"
                     /translation="MTHTVQGERRALCGICSAGCGVIVAYDDHGRIVSVRPDEDAEIG
                     IICRLGEASPEIVYSSDRVLYPLRRAGPKGTHAFERITWDEAYETIVANLTRIREESG
                     PEAVAIYTGSGSFELSFCDIFQPKGVAVSSASSVLFPFGSPNTMGVGALCYVSFAMIA
                     PHVTMGEMYFNMFSDYEQSDLILVWGTNPATDCPPLALRALAGARRRGAEVVVIDPRR
                     TRTVDLVDAEWVPIRPGTDGALALGLANVIVAEELYDADFVANWCHGFEEFATYVQHY
                     RPEVVESITGIPAETVASLARRIARARGASFAMYTGLEYSDSGVQAIRAVFVLWGISG
                     NLDVPGGRCFAMRGNAFPINRSGYIQNPDLKRAIGTDRFPVYTHYRQEGHAIALPDAV
                     LRGRPYRIRALILEAAHMLISWPQTPVWRETLANLDFLVSVDRHLTADAAYADIVLPA
                     TTLYERESYMTYGPVFRLRERVIEPLGEARGDVQIMAELARRLGYGHLYPQSEEEALR
                     HVLKGSGFTLEGVRAAGGTARSPAAMMEYRKWEKGLLRPDGRPGFDTPTGKFEIHSTI
                     LEEYGYDPLPVYTEPGESPLSQPEQAERFPLVFNSGARVTTDFHAQHHSIPSLVRERP
                     EPTVTLNSHDARERGIRDGDRVIVRTARGEIVLRALVTDGIVRGAVEANMGGGCHQAP
                     EAWREGNVNELTDLARYDPISGFPVYKALLCDVARAEGDDGRVAIGTGEVDSVAGAAP
                     PPEAPRIYLDHNATTPLDPAVHQAMAAYLTSNPGNPSSIYREGRDAKVAVESARRSLA
                     RLLNCTARRITFTGSCTEANNTVIKGLAFAGRDGIRREIITSPTEHSAVIEPCRWLEQ
                     FGFRVTFLPVDRSGRVDPADLEGLIGPETLLVSVMVANNETGTIQPVRELVRIAHDHG
                     ALFHTDATQAIGKMPVDAVDLDVDFLTLSGHKIYGPKGVGALYMKKGVRIAPLVHGGE
                     QEGRFRAGTENVAGIVGLGRAAEIAEQHIGRMDAVRHLRDRLEAGIRETVPGARLNGH
                     PTERLANTLNMVLPGYRGESIVLALDRRGISLSSGSACHSGSPAPSHVLLAMGLSAED
                     AHCSIRMTLGAHTTDEDIDRALEAFRDLLHGPGEIIRFVACR"
     gene            complement(12421..13830)
                     /locus_tag="PLI76_04085"
     CDS             complement(12421..13830)
                     /locus_tag="PLI76_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI61243.1"
                     /translation="MDRQQKIVLSVLVLGTLMGALDSTIVILAFPVIADGLHADLLST
                     MWIILVYLLIMASLTVPLGRLGDLFGRPRMFNLGFGLFTVSSFFCGISSSITMLILFR
                     GVQALGGALLESNSGAIIADIFPPNARGAAYGYNSMGWTVGATLGIVLGGIITTFAGW
                     EYIFFINVPIGIVALYLGLRYVKGAARTQASLDLPGMALLTIALSLISYAAIDFAGNG
                     LTTWSTIVAPLGVFFLLVFAWHEVRTRTPMIDFESLNRPILKYSLLAAFFQSLGYLAV
                     IFLITMYLQGIRGLSPLDAALLLTPGYVVGSVLGPFMGRLSDRLGARIIATTGTLVIA
                     AAIFIFMMLRVDSPLSIVLLASFVSGVGTSMFYPANNSAIMANARAGDYGGISGALRM
                     VQNIGILGSFVLAITVASASIPRALAFEIYVGTSHLVGNVAGAFIQGIDAALMVSLLL
                     LLLAGAMSWSRGSEHREAA"
     gene            complement(14122..15516)
                     /locus_tag="PLI76_04090"
     CDS             complement(14122..15516)
                     /locus_tag="PLI76_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOI61244.1"
                     /translation="MNDILTAQCSNNLLTEGSVGKGLWIVALPIIISNFLASILEVVD
                     MYFIGKLGDVSIAGGAMSISIIIVLTTVIFGTVTATAAFVSRAYGSERYERIPVILSH
                     SLYLALGFSAVLAVIGMFWSQDLLLLLGADPEVAAVGARFLSPMLMGMFVFVTLMILT
                     TVFQSTGDSRTPMFVMIGVNIANIVLNPTLIMGLGGLPAFGIAGSAYASIASRATGVL
                     LLVGVMYLLPSKKNGPVRLPKRWTFEPRLLKDIVRVMVPSAVQSGVRSFAFLGMTAIV
                     ALYGTSAVAAYGICQRLDMLGLIFVMGLCTGVAVMVGQNLGAGKVERAEKTVRIAMIV
                     NALFMALVGILYLRSADHLLAFFGATEASLANGIQFMHIVPPSYFVIAMAMTMGFAMN
                     GAGMTRPGMYAAIAGQLIVQVGLAAALVAMEMPLQFIWFAVVCGTVVVFLCDLFFYRQ
                     GAWKTKKLDLGGEN"
     gene            complement(15623..16444)
                     /locus_tag="PLI76_04095"
     CDS             complement(15623..16444)
                     /locus_tag="PLI76_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOI61245.1"
                     /translation="MHAWNPDDYHKHSAAQRAWATELIGKLELAGNERVLDLGCGEGK
                     VTAEIAARLPSGSVLGLDISRDMIAFARMHLPPERYPNLRFAEGDMLDLPFDTEFDIV
                     FSNAALHWVSDHGRVFSGIARALRPGGRVLLQMGGRGNAAPVLAVADDMLAGEPWTTL
                     ASGPPSRYAFYGPEDEREPLRAAGLIPVRVELIGKDMAFDSPDGLAGWVRTTWHLYLE
                     RLPEEVRPAFVAELVARYVEGRPSQDGRIHVPMVRLEVEAVKAAKRWSTGNSSLR"
     gene            complement(16555..16764)
                     /locus_tag="PLI76_04100"
     CDS             complement(16555..16764)
                     /locus_tag="PLI76_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61246.1"
                     /translation="MSIEKMAHLKSLFKISPSSRRTYPHPGLDEVRDLFARAGFVSFV
                     VDTEQLEGETLFVIRAMRDLCRMGA"
     gene            16820..>17233
                     /locus_tag="PLI76_04105"
     CDS             16820..>17233
                     /locus_tag="PLI76_04105"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HOI61247.1"
                     /translation="MSSDPELSRGGYDMNIRELWKQLLQRDEHIRLEVKLGNEIGNSV
                     MQTVCAYANTIGLDGGYLLLGIKEDKSANPKYTIVGVDNPDKLQSDLATQCSSKFNEI
                     LRPKIQSGVLEGKTVICVYTPEAEARVKPIFVKKQG"
BASE COUNT         3230 a         5546 c         5612 g         2845 t
ORIGIN      
        1 agaccaaggc ctgagcgctc ccctgtctgc cccggaagtt ccggttcgac gtcgagagcg
       61 agacctcgcc cggggcaagc agcccgaacg caccccccat gcaggggccg cagcacggcg
      121 cttccacgag ggccccggcc tcgacgaacc gctcgatgag accggcccgg agagccttga
      181 gatactccgt ccgcgatgcc ggtatgacga tcacccgaac actctttgcg aaacggtcgg
      241 cgttccccat cacctccccc gcctcggcga gatcctcgta gcgcccgttc gtgcaggaac
      301 ctatgaagac ctggtcgacc ttcgtccccg cgacatccgc gacatccacg acgttgtcga
      361 cgttgtgggg gacggcgacc tgtggagcga ggtcggtgac gtcgtagtgc cggcgctcgc
      421 gatagaccgc atcatcgtcg cccgcgagat cgaacggctc gatctcgcgg cgggcggcga
      481 catactccca cgtggccgcg tcgggccgga cgatacccgc ctttgcgccc atctcgatcg
      541 ccatgttcgc gcaggtcatc cggcccgcca tattcatcct ctccatcgct ttaccggtga
      601 actcaagcgt catgtaggtc gcgccgtcgg cgccgatatc cccggcaagg gagagaatca
      661 ggtcttttgc ccccacgcgc cggtcgaaca ccccgtcaac ctcaaggagg atgctctcgg
      721 gcacccggaa gtagagcgcc ccgaacttca ggacgaaccc catgtcggtc gagccgatgc
      781 cggtcgcaaa cgctcccgcc gcaccgtagg tgcaggtgtg ggagtcggtc ccgacgatga
      841 tctcgcccgg cgccgcccgc cccttctcca taacgacctg gtggcagacg ccctcgagga
      901 ggtcgtagtt gtggatcccc tgctcctctg caaaccgccg catgaagacg tggttctcgg
      961 cagccggtat cgagtcggcg ggaacctggt ggtcgaagag catgatgatc cgctccggat
     1021 caaagactcg ttctcctccc atctcaccaa agacccgaac cgcaaggggc ccggtgatgt
     1081 cgtggatcat cgccgcatcc agcggggcca tcacgacgtc ccccgcgcgg acggatctgc
     1141 cgcacttcat cgagaatatc ttctctgcga tcgtcgccgc catgagttat cgtgttcctg
     1201 gtacgcgctc cggacgtatc tacctttgcg gggtccggcc tcgtggtgca ggcaatcggg
     1261 gccgtattcg gcgatatttc tcaaggagta tccttatgtg gcctcgcgcg gattgtatag
     1321 ggagaagcaa tgacggatgg accgaccccg gcgatgcggc agtactactc ggtgaagaac
     1381 cggtatcccg atgccatcat cttcttccgg atgggcgact tctacgagac attcggtgaa
     1441 gacgccggcg tggtggcccg cgagctcgat atcaccctga cggcacgggg aaaggacagg
     1501 aaaggcgacc ggatgccgct tgcgggcgtc ccccaccacg ccgccgacgg ctacatcgcc
     1561 cgcctggtgg gcaggggcta caaggtggtg atctgcgatc aggtggagga tccaaagacc
     1621 gcaaagggcg tcgtgaaacg ggaggtgacg cgggtgatca ccccgggaac cctgatcgac
     1681 tcgtcgatgc tcggctcggc cggggcgcgg tacctgatgg cggtcgcccc cgacagaaaa
     1741 gacaccttcg gccttgcgtt cctggacgtc tccaccggcg aattcttcgt ctccgcgggg
     1801 agcggcgggc gcgagtacgc cgacgtcgta tcggaggcgg tcaggtaccg gccatccgag
     1861 gcgatcctcc ccgaggctct cgatgaaggg ctcgccggcc ggctcgagag catcggggtg
     1921 acggtgagcc gttaccgcga cgatgcgttc gatccggatg cagcctgcag gctcctctgc
     1981 gaacagttcg ggacggcgac gctcgacggc tacgggtgcg ctcagatgac gggagccgtt
     2041 gccgcggcag gggcggctct ccattacgcc cgcgagaccc agcagtcgcc cctcccccac
     2101 atcacggggc tctcgacccg ggtcccgtcg gggaccatgg tgctcgacgc gatcaccctc
     2161 cgaaacctcg agatcacgac cggcatccgg ggcgaaggcg acaggagcac cctccttgcc
     2221 gccctcgacg tcacggagac gtcgatggga agccggacga tgcggtcgtt cctggtagcc
     2281 cccctggtca ggaaggcggc gatcgaggag aggctcgatg ccgtcgagtg gctcatcggc
     2341 cacaccgtcg agcgccaggc gctccgcgcg gctctcggcg attttgccga tatcgagcgg
     2401 atagcgggaa ggatcgccta cgggaacgcc ggcccccggg atcttgcgat gctcagggaa
     2461 tcgctttctg cggtcccggg cgtgaaggcg cttcttccgg gcgaccttcc gaccctcgtc
     2521 cgcgaggccc tcgatgcaat gagcgatcac gcctccgcga gcgacctcat cggccgggcg
     2581 atcgtcgatg accccccggc gcttgcaaaa tccggcggca tgatccggga ggggttcaac
     2641 gcgaaactcg acgagctccg gggtcttgca acgaccggga aggactggat cgtggagttc
     2701 cagcagcagg agcgggaacg gaccggcatc aggtcgctca aggtcgccta caaccgggtc
     2761 tttggctact acatcgaggt gacgaaacca aacctccccc tggtgccgcc ggagtacgag
     2821 cggcggcaga cgaccgcgaa cggcgaacgc tacacgatac ccgacctccg ggagaaggag
     2881 gcgctgatag cgaccgcgga ggagcggctt tccgcgctcg aagcggagat ctacgcagag
     2941 ctcgtccgga cgctcgcggc ggaggtcccc ggcctccagg cgaccgcccg tgccgtgggg
     3001 ctgctcgacg tctactcggc cctcgccgag gttgccgccc gctacggcta ctcccgcccg
     3061 gctatcgagg agagcggccg gatcgtcatc cgcgacgggc gccacccggt cgtggagcgg
     3121 cacctcccgg tcccgttcgt cccgaacgac acggaactcg atagcgccgg cgaccagatc
     3181 atgatcatca ccggggcgaa catggcgggc aagtccacct acatgcgggc ggtggcgctc
     3241 tgctgcatca tggcccagat ggggagtttc gtcccggccc gccacgcgac gatcgggatc
     3301 gtcgaccggg tcttcacccg ggtgggggcg tttgacgacc tcgcctccgg gcagtccacg
     3361 ttcatggtgg agatgctcga actcgcaaac atcttgaaca acgcaacgcc gcagagcctg
     3421 gtcgtcctcg acgagatcgg gcgggggacg agcacgctcg acgggtgcgc gatagcaagg
     3481 tcggtggtgg agttcctgca cgggcgggct atcgccggcc cccggacgct ctttgcgacc
     3541 catttccacg acctgatcga ccttgagggg tcgcagaaga gggtgaagaa cttccacttc
     3601 gccgtgaagg atacgggaca ggacgtcgtc ttcctccgaa agatcatacc cggcgcgacc
     3661 gacaggagct acggcgtcca cgtggcccgc ctcgccggtc tcccgaagaa ggtgaccgac
     3721 cgggcgatgg acctcctgaa ggaggcgtcg gtgcgggagc tcccgaccgg ggtgcgggca
     3781 ccccgctaca cccagatgct catcgtcgat cccggggagg gggctgttgc cgagaacccg
     3841 gcggtcgggg agctcaagag gctcaacccc gatgagatga cgccgatcga agcgttgaac
     3901 gccctctgcc gcctccggag gatggcgaac ggagaagggg agcgatgacg aagatccgca
     3961 tactcgaccc cgataccgtg aaccagatcg ccgccggcga ggtcgtggag cggcccgcat
     4021 cggtggcaaa ggagctcctc gagaacgcca tcgacgcggg cgcgacgagc atcctcctcg
     4081 atgtcgggtc ggacatggca ggggtcacga agatccgggt gaccgacgac ggtgcgggca
     4141 tgaccgcaaa ggaggctctc ctcgcgttcc accctcacgc aacgagcaag atacaggata
     4201 tagccgacct ttctaccgtc aggacgctcg ggttccgggg ggaggcgctc gcgagcatcg
     4261 ccgccgtcgc ggaggtgacc ctggtcaccc ggccgcgggg cgccggggcg cttgccggca
     4321 cccgggtcgt cgtccggggc ggcgaggtcg tggagacggg cgatgtgggg gcgccggagg
     4381 gaacgacgat cgccgtcgag cacctcttct acaacacccc cgcccgccgg aagttcttga
     4441 agagcaggaa caccgaactc gctcacctat acggcgtcgt ggagaacctc tcgctcgcgc
     4501 acagggggat tgccttccgg gtggtgcaca acgggaagga gcggatggcg gtccagcgat
     4561 ccggcgagct tatcgccacc atcgccgggc tctacggcgc ggacctggcc cgggcgctcg
     4621 tcccggtcgg gggaaggctc ccgttcctct ccatcggcgg ctacgtctcc cgcccgtcgg
     4681 agagccgggg gaacccgtca caggtcgtca tcagcgtcaa cggccggagc atctcctccc
     4741 gaccgatcgc cgccgccgtc cgggagggtt acggcaccct cctcccgaag gaccgctacc
     4801 cggtggcgtt catcgacctt gcgatcgata ccgatctcgt ggacgtcaac gtccacccga
     4861 caaaacgcga ggtccgcctc tcgcgggaga gggagatcct cgcggcggtc gcggccgcgg
     4921 tagaagaggc actcctcggg cacgaccttg caggcgctac gccggcagag ccggtgcagc
     4981 aggtcctcac gggagagaga cctgaggcgg ccccggcagc ccgtgaggcc gaagcggcct
     5041 acgccgcagg ccaccgcgat atcgcccgct ccgataggca gcttcgccgg acggaggggg
     5101 agggggaaga aaacctcctc cccgccatgg aaccggtcgg gcaggtggcc gcgacctaca
     5161 tcgtggcgga gggggtcgac ggcaccctct acctcatcga ccagcacgcg gctcacgagc
     5221 ggatcctcta cgaccaggtc gcggagcggc gcgatgcggc gcccgggtcg caggagctga
     5281 tcatgcccgt cgtcctctcc ctccccccga aggaatcggc agcgctccgg gacgcgatgc
     5341 cgttcctcgc ggaagagggg tttgaggtgg aggagttcgg ccgggatacc ttcgccgtcc
     5401 gggcggtgcc ggccgccctc ggcgctatgg aggatccgga gagcatcagg gagacgatcg
     5461 ccgatatact cgccggcgag tcccggacga cccccggccg gcgggaggcg gtcacctgca
     5521 tcgtcgcctg caggggggcg gtgaaggccg gcacccttct cacgcacgac cagcagagac
     5581 gcctcctctc acagctcgcc cgtacaaaga ccccctggac gtgcccgcac gggaggccga
     5641 cggtggtggc gtttgatagg cgcaggctcg acggcatgtt cgggcggtga cggagccgtc
     5701 cactacgaat ccctgcagtt cctctccgga tcctgcatag aggggagaac gagggctatt
     5761 tgagccaaat ggcgtatcac aatggattta ttctcgctcg atcagaatgg ctatacgtca
     5821 caggggtgac tcacccgctg cggacgaaag gattgaacca cgtgacatta ctccagcaga
     5881 tcaggctctg gatgcaaacc cggggtgaag ggaagcagtt gcacgattgc gagaacgatg
     5941 ccgtcataga accctacttc gatctctccc ccgaagcgac cctccccccg gagacccgcg
     6001 agaacctgct ggaagaggac aggtgacggg ggcctcccaa aaacgcccta cccgatggat
     6061 cgcctcttac ctccagataa cccaccccca tcacctcttt ttgagaaccc gcccggtgcc
     6121 cggggatgat cgcggggggt cagatgaact caaacgactc cgccacggcg gccgcgtcgg
     6181 cgttgtagct atcgtacccc tcagggaaga ccgtgtgcat gaccgagtag acgatgtcgt
     6241 ctctgacggc ataacggatg atgaaggtgt agcggtcgct ctcgacgatg gggacggagt
     6301 agaccgacct gtacgccggt ataccgtcaa gggcggtctt ctccgtcgag acccacacgg
     6361 ccccgatatc agtcccgagg tcctctgcgt aggtcgcgtt cagcgtctcg aggatcgcct
     6421 cgcgagagct attcccatcc gggagcggga tcgtgttgat gcggacctcc tcgtgcttca
     6481 ccggcgaaga gaaccatacg ctctctcccg attcggtgaa gaaccacccc gccgggcagg
     6541 tgacggcgta gccgtaggtc gtgttgatgt aggtgccgtt ctcgggcagg ggcgccggag
     6601 tcggggcggg agcgggttca ccctctcccg ggcccggagg ttgcggcgcc gcggggcccg
     6661 gcggcaggat cgcgaggacg gcgaccaggg cgaccgcgag aaccccgatg atcagcagac
     6721 tctgtggttt catgctccgg ggtacgtggc ccgggagacc ataaatatcc ttcattgcag
     6781 tggccgttcc ccgggcgatc agggggtcgc ggcaggggtg cagacatcgg caaggaagtc
     6841 gtcgagggta tgcacgtact tcacccggat cccgtagcgc tcctcgatgt acatccccgt
     6901 atcgacggcg accggcctct gccgggctat ccggagaccg caaaagatcc ccgtctcctc
     6961 ccggctctcg acgatgttgt tgttctccgc cgggaggagg aggagggtct ttccgctcgc
     7021 cgcggcggcc ccggccttct cgaggatgcc gccgaccggg ccgatgttgc cgttctcatc
     7081 gatggtcccg gtcacggtca cgctctcgtt gagcgcaaag tcctccatca ccgagaggag
     7141 gagcgcggtc atcaacgctc ctgcactggg gccgtcgatc tcggagaccc cgtcggggct
     7201 ctggatgctg aagatgatgt cgctccccgc aaggtccgcg tgggaacggc cggcggcgag
     7261 gtcgacggca cggtttgcgg catcctgaaa gacgatcccc atcaggggcg tcgtctggac
     7321 gagcacccgc cctcttccgg gcacgatctc gaccgatatg ttcaccatcg ccccctcctc
     7381 gatcatccgg ttcgtggaga acgggccgat gccggtgatc tcgaccttct ggagggtgac
     7441 gggtgcctgc atcgatgccg ttctcccgat cccggggaga gaggccatgg tcgccgccgg
     7501 ggacggaccg gcatccggag ggaacagcgc ggggatatcg tccccgggca cgagagcgac
     7561 ggcaaggagg aagacgttcg caaagagcga aagaacgaag aggatcgtca gggtcttctc
     7621 ccgtacgcgc atatgctgcc cgcataccgt tcatccagat atatactttt cccctggcgg
     7681 atatgcgcac gtacctgccc gccgattcat cgatgaatcc cggccggacc tccccgggcc
     7741 gggggatcct gagccatacc ggccggctct acggggcgcc ctgccgcccg ggagagagag
     7801 gcgagcgcaa accagccgcg ggggtcggag aaccagcgct ccacggcaag cccggcggca
     7861 ccaaagagcc gtccggcgct ctcccgggtg aacttccggg atatctcggt gaggatcgtc
     7921 tccccccgct caaacgccac gcggagcccc agggcaggga tcctgaccgt catcgcttcc
     7981 cgcgccacga ggtgcatctc gatctgcgcg agatccctgt tgtagaacgc acggtgttca
     8041 aagaggcccg gatcgaagtc cgccccgagt tcccggttga ccacggccag gatgttcctg
     8101 ttgaacgcgg cggtcacccc ggcggtgtcg ttgtacgcgg cctcgagcac cgggaccggt
     8161 ttcaccatat cgagcccgac caggaaccgg tcgtcctcct ccatcatggc agccacgttc
     8221 acgagcaccc ggtgcgcctc agggccggta aagttcccga cggtgcctcc aaaaaacgtg
     8281 atgagcctct ttctcccgcg ggggatgcgc tcgatatcgc gggtgaaatc gcccacgatc
     8341 ccgaggacgt gcaggtgcgg gtacccccaa agcagcccct ctgttgcggc ggccagggcc
     8401 gactcgctca catccaccgg gatataccgc aggccttcgc cttccggccc cggcgccgca
     8461 tcgatgagca gggagatctt tttgttcgct cctgacccca gttcaacgag gtcgccctcc
     8521 ccaaaggatt ccatgagcgc cccgcacgcc ccgcaaagga tggataactc aagcctggtc
     8581 ggataatact cagggagccg gcagatctcc tcgaacaact gcgatccccg ggcgtcgtag
     8641 aagtaccggc tcggtatgta cttctgctcc gcggtcatcc cggagaggat atccgaggcc
     8701 aggtcgtcac ggtatgcaga gatgaggtag ttccgaagct cgctccgtac ggaaggagtg
     8761 ctcaggcgac ccgtcatgcc cgacaccgat ctcgattatg gcatatatat tgcccgctcc
     8821 cccgatggcg cggagatctc cggtcaccgg caggcgacga accggatgat ctcccccgga
     8881 ccgtgcagga gatcgcggaa ggcctcgagt gcccggtcga tatcctcatc cgtcgtgtga
     8941 gcgccgagcg tcatccggat cgagcagtgg gcatcctctg ccgaaaggcc catcgccagg
     9001 aggacgtggg acggcgccgg agaacccgag tggcaggctg agcccgagga gagggagata
     9061 ccccgccggt cgagggcgag gactatcgat tcgccgcggt agccggggag caccatattg
     9121 agcgtatttg cgagccgttc ggtcgggtgg ccgttcagcc gggccccggg aaccgtctcc
     9181 cggatcccgg cctccagccg gtcccgcagg tgccggacgg catccatacg accgatgtgc
     9241 tgctccgcga tctccgcggc cctcccgagc cccacgatcc cggcaacgtt ctccgtcccg
     9301 gcccggaacc ggccctcctg ttcgccgccg tggacgagcg gggctatgcg gacccccttc
     9361 ttcatataga gcgctccgac gcccttcggg ccgtatatct tgtgcccgga gagggtcagg
     9421 aagtccacgt cgaggtcgac cgcatccacc ggcatcttcc cgatcgcctg ggtcgcgtcg
     9481 gtgtggaaga gggcgccgtg gtcgtgggct atccgcacca gttcccggac gggctggatc
     9541 gtcccggtct cgttgttcgc caccatgacg gagacgagga gagtctccgg gccgatcagg
     9601 ccctcgagat cggccgggtc gacccggccg gagcggtcca ccggcaggaa cgtgacgcga
     9661 aacccgaact gctcaagcca ccggcagggc tcgatgaccg cggagtgctc ggtcggggag
     9721 gtgatgatct cccttcttat cccatcccgg ccggcaaagg caaggccctt gatcaccgtg
     9781 ttgttcgcct cggtgcagga gccggtgaag gtgatccgcc gggcggtgca gttgagcagg
     9841 cgggcaaggc tccgccgggc ggactcgacg gcgaccttcg cgtctctccc ctcccggtat
     9901 atgctcgagg ggttgcccgg gtttgatgtg aggtacgcgg ccatcgcctg gtggaccgca
     9961 gggtcgaggg gggtggtggc gttatggtcg aggtatatcc ggggtgcctc cggcggcggc
    10021 gccgccccgg cgacggagtc gacctcgccg gtcccgatgg cgaccctccc gtcgtcgcct
    10081 tcggccctcg ccacatcgca gaggagggct ttgtagacgg ggaatcccga tatcgggtcg
    10141 tatcgggcaa gatcggtcag ctcgttcacg tttccttccc gccaggcctc cggcgcctgg
    10201 tgacacccgc cgcccatgtt cgcctcgacg gcgccccgga cgataccgtc ggtgacgagt
    10261 gcgcggagca cgatctcgcc ccgcgccgtc cggacgatga cccggtcgcc gtcgcggatc
    10321 ccccgctctc gggcgtcatg ggagttgagg gtgaccgtcg gctccggccg ctccctgacg
    10381 agcgagggga tgctgtggtg ctgggcgtgg aaatcggtcg tcacccgcgc tccggagttg
    10441 aagaccagag ggaaccgctc tgcctgctcc ggttgcgaga gagggctctc gccgggctcg
    10501 gtgtagaccg ggaggggatc gtagccgtac tcctcgagga tggtcgaatg gatctcgaac
    10561 ttccccgtcg gcgtatcgaa cccgggccgg ccgtcgggcc ggaggaggcc tttctcccac
    10621 ttccggtact ccatcatagc cgccggcgac cgggcggtcc cgccggccgc cctgacgccc
    10681 tcgagcgtga accccgagcc tttcaggacg tgccggagag cctcttcttc cgactgcggg
    10741 tagaggtggc cgtaaccgag gcggcgggcg agttccgcca tgatctggac atcgccccgg
    10801 gcttcgccga ggggctcgat cacccgttcc ctgagccgga agaccgggcc gtaggtcatg
    10861 taggactccc gctcatagag ggtcgtcgcc gggaggacga tgtcggcgta ggcggcatcg
    10921 gccgtcaggt gccggtccac agagaccagg aagtccaggt tcgcgagggt ctcccgccag
    10981 accggcgtct gcggccagga tatcagcata tgggcggcct ccagtatcag ggcgcgtatc
    11041 cggtagggcc tcccgcgaag gaccgcatcc ggcagggcga tcgcgtggcc ctcctgccgg
    11101 taatgggtgt agaccgggaa ccggtcggtc ccgatagccc gcttcaggtc ggggttctgg
    11161 atgtagccgg agcggttgat ggggaaggcg tttcccctca tcgcaaaaca ccggccgccg
    11221 gggacgtcca ggttccccga tatcccccag aggacgaaga ccgcccggat ggcctgcacg
    11281 ccggagtcgc tgtactcaag ccccgtgtac atggcaaacg atgcgccccg ggcgcgggcg
    11341 atgcgccggg caagcgacgc cacggtctct gccggaatcc cggtgatgct ctccaccacc
    11401 tcggggcggt agtgctggac gtaggttgcg aactcttcaa aaccgtggca ccagttcgcg
    11461 acgaaatcgg catcgtagag ttcctccgcg acgatgacgt ttgcgagacc gagcgccagc
    11521 gctccatccg tccccggccg gatgggcacc cactccgcgt ccacgaggtc caccgtccgg
    11581 gtgcggcggg gatcgatgac gacgacctcc gccccccgcc ggcgcgcccc ggcgagagcc
    11641 cggagggcaa ggggcgggca atcggtggcc gggtttgtcc cccagacgag tatgaggtcg
    11701 ctctgctcgt agtcggagaa catgttgaag tacatctccc ccatggtcac gtgcggcgcg
    11761 atcatagcaa aggagacgta gcagagcgcc ccgacgccca tggtgttcgg ggagccgaag
    11821 ggaaagagga cgctcgatgc ggacgagacc gcgacacctt tcggctggaa tatgtcgcag
    11881 aacgagagct cgaagctccc cgacccggtg tagatggcga ccgcctccgg cccggactcc
    11941 tctctgatcc gggtcaggtt cgcgacgatc gtctcgtatg cctcgtccca ggtgatccgt
    12001 tcaaacgcat gggtgccttt cggccctgcg cgccggagcg ggtagagcac ccggtcgctt
    12061 gagtagacga tctcgggcga ggcttccccg agcctgcaga tgatgcctat ctcggcatcc
    12121 tcgtcggggc ggacggagac gatccttccg tggtcatcgt aggcgacgat tacgccgcac
    12181 cccgcagagc atatcccgca gagcgcccgg cgctccccct gaacagtgtg agtcatgtgt
    12241 gatccctgca tggtataccc cggttgcacg aaccgccccc tggggccggt gcggcataat
    12301 ggttaccatg gccggatcag gagaggccgg caggagtctt tccggggatg ccgtggctat
    12361 ccggtatcct gccgctaccc gcacgaccac cggatcgggc ggccggggta tgggggcggg
    12421 tcatgccgcc tcgcggtgct ccgacccgcg ggaccaggac atcgccccgg caaggaggag
    12481 gaggaggagg gagaccatga gcgcggcatc gatgccctgg atgaatgccc cggccacgtt
    12541 ccctaccagg tgggatgtgc ccacgtagat ctcgaacgca agagcgcggg ggatcgaggc
    12601 ggaggccacc gttatggcaa ggacgaaact gccgaggatg ccgatgttct gcaccatccg
    12661 gagcgcgccg gatatgcccc cgtaatcccc cgcccgcgcg ttggccatga tggcgctgtt
    12721 gtttgccggg taaaacatcg atgtgccgac accggagacg aacgacgcca ggaggacgat
    12781 ggagaggggg gagtctaccc tgagcatcat gaagatgaag atcgccgccg cgatcaccag
    12841 cgttccggtg gtggcgatga tccgtgcccc gaggcgatcc gagagccttc ccatgaaggg
    12901 gccgagaacg ctccccacga cgtaaccggg cgtgaggagg agggcggcat ccagggggga
    12961 gagcccgcgg atcccctgga ggtacatggt gatgagaaat atgaccgcga ggtagccaag
    13021 gctctggaag aatgcagcga gaaggctgta tttaaggatc ggcctgttca ggctctcgaa
    13081 atcgatcatg ggggtccggg tgcgcacctc gtgccaggca aataccagga ggaagaagac
    13141 ccccaggggg gcaacgatcg tcgaccacgt cgtgaggccg tttccggcaa agtcgatcgc
    13201 ggcgtaggat atgagcgaga gggctatggt aaggagagcc atgccgggga ggtcgaggga
    13261 tgcctgcgtc ctggctgcac ccttcacgta gcgcagacca aggtagaggg cgacgatgcc
    13321 gatggggacg ttgatgaaga agatgtactc ccatccggca aaggtggtga tgatgccccc
    13381 gagcacgatg ccgagcgttg caccgaccgt ccagcccatg gagttgtagc cgtatgctgc
    13441 cccgcgtgca ttgggaggaa atatatcggc tatgatggct cctgagttcg actccaggag
    13501 tgcccctccc agggcctgga cgccgcggaa gaggatcagc atggtgatgg atgaggagat
    13561 accgcagaag aacgaactga cggtgaagag gccgaacccg aggttgaaca tgcggggccg
    13621 gccgaagaga tccccgagtc tcccgagcgg gacggtcagg gacgccatga tcaggagata
    13681 gacgaggatg atccacatgg tgctgagaag atctgcgtgg agcccatccg cgatgacggg
    13741 aaaggccagg atgacgatgg tgctgtcaag tgcccccatc agggtgccga gcacgagaac
    13801 agagagcacg atcttctgct gccgatccat atcgagaggt tagattgtgt atccatatag
    13861 gggttcccaa attggcgtcc gtgcatatcg gtcttcctgc ccatcgtgaa ggaggagagc
    13921 ccggaggtga gaacgggttg caaccgcccg atgcagcgag agcgcaggca ccgggtcttg
    13981 ctcatcttga cgaccggcac ccttcgtgtc gagcgcaaaa ccgcgcagga agatcgccag
    14041 cctgaccccc cgcgagggtt cttcaagacc tcagcggtgt gccggttcag tcgaaaaaaa
    14101 ggttgtcggg ggttttccgg gtcagttctc cccgccgaga tccagtttct tcgtcttcca
    14161 ggccccctgc cggtagaaga agagatcgca caggaagacc accacggtgc cgcagaccac
    14221 cgcgaaccag atgaactgca gcggcatctc catcgccacc agggccgcgg caaggcccac
    14281 ctgcacgatc aactgtcccg cgatcgccgc gtacatgccc ggtctcgtca tccccgcacc
    14341 gttcatcgca aaccccatcg tcatcgccat cgctatcacg aagtacgacg ggggaacgat
    14401 atgcatgaac tggatgccgt ttgcaagcga cgcctcggtc gcgccgaaga acgccaggag
    14461 atgatccgca gaccgcaggt agaggatgcc caccaatgcc atgaacaatg cgttcacgat
    14521 catcgcaatc cgaaccgttt tctccgccct ctccactttc cctgctccca ggttctgccc
    14581 gaccatcacc gcgactcccg tgcagagccc catcacgaag atcaggccca gcatatccag
    14641 gcgctggcag ataccgtacg ccgccaccgc cgacgtgccg tacagcgcca cgatcgccgt
    14701 cataccgagg aacgcaaaac tcctgacccc gctctgcacg gccgacggca ccatgactct
    14761 cacgatatcc ttgagcagcc gcggctcaaa cgtccaccgc ttcgggagcc tgaccggacc
    14821 gttcttcttc gacggcagga gatacatcac cccgacgaga agcagcacgc cggttgcacg
    14881 cgacgcaatg gacgcatacg cggatccggc tatgccgaac gccgggagac cgccaaggcc
    14941 catgatcagc gtcgggttca ggacgatatt cgcgatgttc accccgatca tgacgaacat
    15001 cggcgtcctc gagtcccccg tgctctggaa cacggtcgtc aggatcatca gggtgacgaa
    15061 gacgaacatc cccatcagca tcggggagag gaagcgtgcc cccaccgctg caacctcggg
    15121 atcggctccg aggagcagga gaaggtcctg cgaccagaac ataccgatga ccgcaaggac
    15181 cgcggagaag ccaagtgcca ggtacagcga atgcgagagg atcaccggga tgcgctcata
    15241 ccgctccgac ccgtacgccc gcgacacgaa tgcagccgtt gccgtcacgg tcccgaagat
    15301 cacggtagta agcactatta tgatggatat gctcatcgct ccgccggcaa tcgagacatc
    15361 gccgagtttg ccgatgaagt acatatccac cacctccagg atgctcgcaa ggaaattgct
    15421 gatgatgatc gggagggcca caatccagag gccttttccg accgatccct cggtgagaag
    15481 attattggaa cattgtgccg tcaagatatc attcattata cggggcgaaa gacgataaag
    15541 tttgaccgac cgcctcaacc tgcatttttc cgtaaatgtg aaaagaagcg ttatggcttt
    15601 tgatcgggct atggaggata tgttatcgaa gagagctgtt gccggtggac catcgcttcg
    15661 ccgctttgac ggcttctacc tcgaggcgca ccatgggaac gtggatgcgg ccgtcctgcg
    15721 aggggcgccc ctccacgtac cgggccacca gttcggcgac gaacgccggc cggacctctt
    15781 cggggagccg ctccaggtac aggtgccagg ttgtccggac ccatcccgcg aggccgtcag
    15841 ggctatcgaa ggccatatcc ttcccgatca gctcgacccg cacggggatg agccctgccg
    15901 cccggagcgg ttcacgttcg tcttccggcc cataaaaggc atagcgggac ggcgggccgc
    15961 ttgcaagcgt ggtccagggt tccccggcca gcatatcgtc tgcaaccgcg aggaccggcg
    16021 cggcgttccc tctcccgccc atctggagga gaacccgtcc tcccggccgg agggcgcgtg
    16081 cgatccccga gaagaccctg ccgtggtcgg agacccagtg cagggcggcg ttcgagaaga
    16141 cgatgtcgaa ctccgtatcg aacggcagat cgagcatatc gccctcggca aaccggagat
    16201 tcggataccg ctccgggggc aggtgcatcc tcgcgaaggc gatcatatcg cgggatatgt
    16261 caaggccgag caccgagccc gaggggaggc gggcggcgat ctccgcggtc accttcccct
    16321 cgccgcagcc gaggtcgagc acccgctcgt tgccggcaag ttcgagcttt ccgatcaact
    16381 ccgtcgccca agcccgctgg gcggcggagt gcttgtggta gtcgtccggg ttccacgcgt
    16441 gcatcacagg aataccctct ccgctggtac gggtaccgct gccgcagggc atcaagccat
    16501 cggtgaggga gcgcgccgga aaagtgcatc agcgggtttt gccccgggga ccactcacgc
    16561 ccccatccgg cagaggtccc gcatcgcccg gatcacgaag agggtctcgc cctcaagctg
    16621 ctcggtatcg acgacgaacg agacgaaccc cgcccgggca aagaggtccc ggacctcatc
    16681 gagcccggga tgtggatacg ttcgccttga cgaaggagaa attttgaaaa gtgactttaa
    16741 gtgcgccatt ttctcgatag acacgacatc cggacttctg tagaaagata atatggttct
    16801 acgaacgtaa cgtatgctaa tgagctccga tcctgaattg agccgggggg ggtacgatat
    16861 gaatatccgc gagttatgga aacaactcct acaaagggat gaacatattc ggttggaggt
    16921 gaaactcggc aacgagatcg gaaactctgt gatgcagacg gtgtgtgctt atgcaaatac
    16981 gattggactt gatggcgggt atcttctcct cggcatcaag gaggataaaa gcgccaatcc
    17041 aaaatatacc attgtgggtg tcgataatcc agataaactt cagagtgatc tggcaactca
    17101 gtgttcgtct aaattcaatg aaattctccg cccaaaaatc cagagtgggg tactggaggg
    17161 aaaaacagta atctgcgtat acacacctga agcagaagca cgagtaaaac caattttcgt
    17221 gaaaaaacaa ggg
//
LOCUS       DAOSEE010000034        17076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1364_length_17076_cov_234.056225, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000034 DAOSEE010000000
VERSION     DAOSEE010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 17076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17076
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1364_length_17076_cov_234.056225"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PLI76_04110"
     CDS             <1..227
                     /locus_tag="PLI76_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61248.1"
                     /translation="GWMRYSGSLSVDPDLIVRTFFEVGVAVFMLELLYRFLVGLRYPG
                     HVRVTLKPAGFLRVYTDDPERVAALLSSPR"
     gene            321..1139
                     /locus_tag="PLI76_04115"
     CDS             321..1139
                     /locus_tag="PLI76_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOI61249.1"
                     /translation="MTYSSAGAHPPPPPLIEFKNVTVTRGGRILLDSVSLAIREGENI
                     AVLGPNGAGKSSLIRAITREYYPSRRGEDVTFRFRGQDRWDAFDLRSHIGIVSGDLQH
                     TFTRGISGREVVLSGFFSSIGLFFSHEVTGAMEEKADEILAFLGVAHLADRLMLEISS
                     GEARRLLIGRALVHDPGTLLLDEPTNSLDLAALHAFRATLRRIARSGTGIILVTHNLH
                     DIIPEIARVILMRDGAIRMDGRKAEVLTDEAIGGLFCVPVHVVSEGGYYYATGY"
     gene            1251..2120
                     /locus_tag="PLI76_04120"
     CDS             1251..2120
                     /locus_tag="PLI76_04120"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HOI61250.1"
                     /translation="MVEIGYKLFTGSHSAPELVKNARLAEDAGFSFLNISDHYHPWIS
                     THGRAGFAWCTIGSISQATSRIKVSTGVTCPLVRYHPAIVAQAAATAATLMPGRFELG
                     LGTGENLNEHITGSVFPTSEPVRLDMLREAVSIIRMLWKGGVQDYCGAYYALDNAQIY
                     ELPRDLPPIRISAQGPMAAEVAGDIGDALIHFEQKPEEIIEAFRASGGEGKSCMIETA
                     ACYAGSEEEAKRTAYEWFPMEELRTAPVRSEKRWIGVPALRRRSLRTPAECPPPRCHT
                     GRPAPTGIRRRRP"
     gene            complement(2023..3282)
                     /locus_tag="PLI76_04125"
     CDS             complement(2023..3282)
                     /locus_tag="PLI76_04125"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOI61251.1"
                     /translation="MRRKVPAIALITLAAVIFACGCTGTTTTEQAEVQENVVGVLLPL
                     TGDFAESGAASRAAVEVAAGDINDYFASIGSDHRVRPIIEDTATDPAVALEKLKALDA
                     QGIRMVIGPGCSAELEAVRAYADAHGILIVSTMSTAPSLAIPGDNIFRLVSPDTYQAD
                     AMAYYLEEEGIAAVVPVWRGDIWGDELRNLTTTAFKAQNGTVYDGVRYTPGQKDYAGT
                     MADLDVQVGKAIAAHGQENVCVYAVTLNEIAPIMTRAAETQNLTGVRWYGCDGNVLLG
                     TLATPGDAARFAAQTNFTGPTFWQQNLAAAHEATFQKIQNRLGRQPDGYSLASYDALW
                     LLTLTGTQTASAETPKLKIALTKVSDTFAGPFCNVVGLNAAGDRSTVHYCFWSIKADG
                     DGYRWVPDAQYDIWVAGTPPVFEAIGA"
     gene            complement(3427..5595)
                     /locus_tag="PLI76_04130"
     CDS             complement(3427..5595)
                     /locus_tag="PLI76_04130"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOI61252.1"
                     /translation="MIKRPPLSEADRIWFLALICIAIIGISSAGCIATTTSADPIRIG
                     VLLPGSGPVAVHSLDGLTWAAGAMNRQGDRTIELVYRDTSAGNISAYAEELAGRDDIA
                     IVIGPATSTELIEIAPLVTGRGKLLISPSVTGEIDDNSLFWRTCPTDDRQVEAMFRIL
                     SETGVRNVSLIVANAPYGETFARFAPAAAAANRIRITGAVSVDASSDFEEIAMRIGEE
                     EPGTVVAAVYPEEAVRIADALSRAGSTAGLFLTDAARSPYLLERLGERAEGIQGTALS
                     SDPTTGYAIAYEELFGEAPPSFAAQTYDALLLAVYTAARQEAAPGEPLAASLRWVVSG
                     DGIAKGWDPEEAGEAAAMIRAGAHPAVTGASGPLRFGEHSSEGPLAGYYTRWTVEGGA
                     FCESTPVSSAGGEPGLPGLSYDPAAGEGVRPVVWMVYPMAKGDRSFADSAYRGLFRAQ
                     ESFSFTKRECTYADIPLLDAVFSLQNFTEKPDLVITEGFEFTDASRAWAEANPDIRFV
                     TLEQAEFGLPNACDVMMVPYGASYLAGVMAANITRTGKIGAIAGAPVPVIDPFVRGFT
                     EGAAAYDPSVDVTVRYVGENAEGFGMPERAGALARDLRAEGVDVILMIAGASNIGIVD
                     TARETGDIYLIGEDTDQSYLAPNLVIASVVKQIDAIVYRAVANEINGTFRGGREVRTL
                     EDDGTMLFVSPRFDAYAGVVADWRERAVAAEGEYLKTALL"
     gene            5948..7165
                     /locus_tag="PLI76_04135"
     CDS             5948..7165
                     /locus_tag="PLI76_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61253.1"
                     /translation="MNKNACVPAVRDLMAEFAGRTGLDPPLANPRRYLWTDAYAVCNY
                     IELYRRTEEETYRDLALRLVSQVHHTLGRHRADDRRTGWISGLSDGEGEVHPTIGGLR
                     IGKPLPERRSGEPFDERLEWERDGQYYHYLTRWMHALNRVSRATNDPAYIRWAVELAR
                     AAHAAFAYLPPSGGKKRMYWKMSIDLSRPLVPAMGQHDPLDGFVTYSELQAAARPLHL
                     DLSPEIADMAGICRGTVQATDDPLGIGGLLFDAGRIAQSMDRSETDRADLLCSVLDAA
                     LAGLAAFARGGSLRYPVEYRLAFRELGLAIGLTGVTGRIREDPEIFGRGALERRAETL
                     MGYAPLADAIVEFWADERNREAGNRAGNPEINMVMLATALAPGEFLAVSLRDNHTLLC
                     RGGEAGSRRMPIR"
     gene            complement(7155..7691)
                     /locus_tag="PLI76_04140"
     CDS             complement(7155..7691)
                     /locus_tag="PLI76_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61254.1"
                     /translation="MEGEYPWYTLVTAAEPLAQGDILHSVPFISPAGDLRGDSRVDCE
                     EYDVIVMSQSCDLIEQKVDSVILSSIFSLDEHITEIARSDSKKAVVRTKNKLRKGEMI
                     AYHLLDRCTIPGHESGYIFVDLKNPRSLPLEFVQRLAAGLPYRVRLCPPYREQLSQAF
                     ARVFMRVGLPSTIPEFTE"
     gene            complement(7705..8097)
                     /locus_tag="PLI76_04145"
     CDS             complement(7705..8097)
                     /locus_tag="PLI76_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61255.1"
                     /translation="MPSLIERPAGRRPAGYSPSTQIVRPYDHFGEVSSTRTMVLARPA
                     GAHHCTRSGVCELSLAYESSSVIHQALKSSSTTYLKERGAGDEEIPGSEDAHIVRLPP
                     KRRMPLIVQVVTRRRGRITNYDHTEIDL"
     gene            complement(8085..8897)
                     /locus_tag="PLI76_04150"
     CDS             complement(8085..8897)
                     /locus_tag="PLI76_04150"
                     /inference="COORDINATES: protein motif:HMM:TIGR04255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04255 family protein"
                     /protein_id="HOI61256.1"
                     /translation="MREDIRQAVAHMGTAKLQNPPLIEVIFEIRWDLQEREDGVRFDP
                     HAKIFPGRLSSALGEEYPVYEELPTSDIPDEISGYIVQHRFRRAKGSWPLVQVGPGIV
                     TLNDIESYEWQDFQRRIEGVLDALFQEYPETSLLATNEVVLRYLDAYPFDFEKENVAE
                     FLSAKLGVQNQISPAFFEAIGVSPVPIGVDFKYSFPSQRPPGYLHVGFSRGRVKGRDS
                     VIGETIVQSPETDGPRTKDEILSWVSEAHALTHTWFFTMIEGDLLEEFACRR"
     gene            9012..9434
                     /locus_tag="PLI76_04155"
     CDS             9012..9434
                     /locus_tag="PLI76_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOI61257.1"
                     /translation="MATPRLMEYFNRQPRIGILSTANRDGKVDAAVFGSPQMTDEKTV
                     VMALGENRTFEYLRENPSAVFTIVESGKTIADWKGLRVYLWMKEYATSGEMLETYKKE
                     VARAVGDEAAKMVHAVVTFEITEVRPLVDMGQGWEGSL"
     gene            complement(9453..10205)
                     /locus_tag="PLI76_04160"
     CDS             complement(9453..10205)
                     /locus_tag="PLI76_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI61258.1"
                     /translation="MPGWFEGVSRDIGAAWAIARKDMKIYYIKPSILVSSLLFPFVMF
                     LAFAVGREAAAGTLIPGLLAVTLLFSASSIEPVSIPIERRTKTFDRLLSAPISIRAIV
                     LGASLSGALFASAIGVATTVIALIVLNAGVMNVAALLLGFALTSLCFSTMGTLFAAYR
                     TENVGEVMSLLNLVRLPLLFISGVFIPLASMPGWGQSIAVFSPLTYAHDLMLFAFDGT
                     TLFGPVIDVAALVLAIVIFQAAGIFLYQRSRE"
     gene            complement(10189..11160)
                     /locus_tag="PLI76_04165"
     CDS             complement(10189..11160)
                     /locus_tag="PLI76_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOI61259.1"
                     /translation="MDAIEVIGLTKRFGDNVAVKGIDFAVKMGETFGFLGPNGAGKTT
                     TIRMLTGMTKITSGRALIHGHDIARDTLAAKRLMGVVPETSNIYDELSAWDNLIFTGR
                     LYHLKKAEIERNARDLLDTFGLYERCHEPAGGFSKGMKRRLTLAMGLVNNPGVLFLDE
                     PTSGLDVQSRRLIRDVVQDLARKNVTIFLTTHDIEEANVSCDRVAIINRGTIAAIDAP
                     EKLKQTFESLQSIEISFDAVRPGLLEALRTLPQVNDVRKEGDKFRLYTADPPEVLSSL
                     CTFTQEQNMRPISVTTRGPSLEEVFIRLTGLSVGTKGEVRETDAGMV"
     gene            complement(11228..12199)
                     /locus_tag="PLI76_04170"
     CDS             complement(11228..12199)
                     /locus_tag="PLI76_04170"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOI61260.1"
                     /translation="MKRIGILTSGGDAPGMNACIRAAVRAALANNLTVVGIRRGYTGL
                     IATDTVPLDRAAIRNIIHQGGTILETSRSPDLYTREGRLRVAEAIGKMGLDGVILIGG
                     DGTFHGASLVAADADTAPLVGVPGSIDNDVYGTDYCIGFDTAANCAVEAIDRIRDTAR
                     SHERLFFLEVMGRTAGFLALESGIAGGAEELIIPEEETSIAPMSERIRKGFDIGKKSA
                     IVVVAEAKTPGVSFQIAGEVKKHLDLDSRVVILGHLQRGGPPTLRDRVLGSSLGVAAV
                     EALLEGRSECMVGEVGGALAYTPLEETWKKKKPLNENLRRIFSILSD"
     gene            complement(12196..13407)
                     /locus_tag="PLI76_04175"
     CDS             complement(12196..13407)
                     /locus_tag="PLI76_04175"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOI61261.1"
                     /translation="MQKAIPRRKKTVRDIDVTGKRVLVRADFNVPLGEDGAIEDDTRI
                     RASLPTIRYLSERDARVILCSHLDRPQGVVVERLRLALVANRLSVLLGRPVTALRDCI
                     GPEVESAVAAMSGGDVVLLENLRFHPEEKTDDPAFAEELASLADIYVNDAFGASHRAH
                     ASIVGVPEHLPAVAGLLLAKEVDTFTRILRDPERPFAAVIGGAKVSDKLGVLDNIMPR
                     VNLLIIGGGMAATFLASRGYGTGRSHVETDRLDDVRQLSAKAMELGVRLLLPRDAVVT
                     ERIEAGAPTQVVPVAKIPDDWVIADIGPETAGEFCRELEGCRTAVWNGPMGVFEIPEF
                     AEGTRRIAATLANLSGTTVVGGGSTADAVARFGLADKMTHVSTGGGAALTVLAGKSLP
                     GVETLEDAEGS"
     gene            complement(13651..14040)
                     /locus_tag="PLI76_04180"
     CDS             complement(13651..14040)
                     /locus_tag="PLI76_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HOI61262.1"
                     /translation="MRGTATQAKARTIARGKDVVDSSVKNPRGEDLGSIKDVMIDINE
                     GCIAYAALSFGGIMGLGDKLFAIPWAALQYNAADDYFVLDVPRDQLENAPGFDKNNWP
                     TTAEREWLTRMYSHYGYPPYWEHHSGR"
     gene            complement(14183..15949)
                     /locus_tag="PLI76_04185"
     CDS             complement(14183..15949)
                     /locus_tag="PLI76_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HOI61263.1"
                     /translation="MSELLTILFPILVGSCAVSALITYSLGAYVLGQNPSSTINRLFF
                     ALALSATCWALGEFFIWQSTSYDGYQFWLRISSFWSFVIALTVHFTLVFTNHPFARED
                     KRWILLVILYLPALLIALIGFSTNLIYTVVFQPGMGYIYQPAAASPAYLASRAYITLA
                     IVWGLYVGISSLRRVRTRRERRQHQLILTGIVTAAGFGAISGLVLPALGVYTVNVVFI
                     GFVLLSLLISYAIHRHGLFALSPQTAVVDILKVMPDGLLLADMDGRVVTANASAAEIF
                     GVAEADLPGQPVRRFIPDTTCDTIRAAIAEQGRFSDLEATPGGREGRTISISGAPVRN
                     PDGESAGVVLIIRDITRRRAAETALRTANQKLSLLSQVTGHDIGNDITGLAWYLNLLS
                     EDRTHPDAGTYLTRSVDIVEDIAKHLAFSRECQMLGAYQSVWQPLGPMIEGAVGSIHH
                     PGVEIAVRVAPVEVYADPLSPKVVYNLLENALRHGGGITRICITTAEGADGMIVVAFE
                     DDGVGVPDDEKERIFCYGYGKNTGFGLAFSRDVLSVTDIAIRETGTAGRGARFEILVP
                     PRAWRRFEGALADPQDAQPAGQ"
     gene            16272..>17076
                     /locus_tag="PLI76_04190"
     CDS             16272..>17076
                     /locus_tag="PLI76_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOI61264.1"
                     /translation="MSGREYVHGYTGREAERLRDQAETLTGLLHGDTRYPAGSEVLEA
                     GCGTGAQTVILARNSPEARITSIDISAASLDAARARVLAEGMKNVTFRAGDIYDLPCP
                     PGSFDHIFICFVLEHLAEPLRALERLRPLLRAGGTITVIEGDHGSAYFHPDSPRARRA
                     IGCLVDLQREMGGNALIGRELYPLVTGAGYRDVRVSPRTVYADATRPDLVQGFTRLTF
                     TAMVEGVGEDAVGRGMMSREDWEQGIRDLYRTAEPDGVFCYTFFKATGRR"
BASE COUNT         3100 a         5359 c         5433 g         3184 t
ORIGIN      
        1 ttgggtggat gcgttattcg ggcagcctgt cggtcgatcc cgatctcatc gttcgcacct
       61 tctttgaggt cggcgttgcg gtgttcatgc tggagttgct ctatcgcttc ctggtcggtc
      121 tgcggtatcc cggtcacgtc agggtcacgc tcaagcccgc cgggttcctc cgcgtctaca
      181 cagacgaccc ggagcgggtt gcggcgctgc tcagttcccc gcgatgacgg agcatacctt
      241 ttgaagagaa ccgtccaacc tacacagcga catccaatgg aatccccgta tcgcaaaccc
      301 cacccgacgg agcggccggc atgacttata gcagcgccgg cgctcacccc cctcccccgc
      361 cgctcatcga gtttaagaac gtcaccgtca cccggggcgg gagaattctc ctcgactccg
      421 tatcgctcgc gatccgcgaa ggagagaaca tcgccgtcct cggcccgaac ggtgccggga
      481 aatcgtcgct catccgggcg atcacccgcg agtactaccc ctcccgtcgg ggagaagacg
      541 tcacgttccg gttccgggga caggaccggt gggacgcttt tgacctccgg tcgcacatcg
      601 gcatcgtctc cggcgacctc cagcatacgt tcacccgggg catatcgggc cgcgaggtcg
      661 tcctctcggg attcttctca agcatcgggc tcttcttctc ccacgaggtg acgggcgcga
      721 tggaggagaa agccgacgag atcctcgcgt ttctcggcgt cgcccacctt gccgaccgcc
      781 tgatgttgga gatctcctcg ggcgaggcgc ggaggctcct gatcgggagg gcactcgtcc
      841 acgacccggg caccctcctc ctcgacgagc ccacgaacag tctcgatctc gccgcgctcc
      901 acgccttccg cgcgacactc cggaggattg cacggtcggg caccggcatc atcctggtga
      961 cccataacct ccacgacatc ataccggaga tcgcccgggt catcctcatg cgagacggcg
     1021 ccatccggat ggacgggaga aaggcggaag tcctgaccga tgaggccatc ggcgggctct
     1081 tttgtgtccc ggtgcacgta gtgagcgagg gcggctacta ctacgcgacg gggtactgaa
     1141 cgccccccgg gtaccgccgt ctcgatcagc acaacgttta agatcgatga cgttccccct
     1201 cgccgctcat accccggcac gagggtgccg gggaagagga gagagaaggc atggtcgaga
     1261 ttggctacaa actcttcacc gggtcgcata gcgcacccga gctggtgaag aacgcacggc
     1321 tcgccgaaga cgccgggttc tcgtttttga acatcagcga ccattatcac ccgtggatct
     1381 cgactcacgg gcgtgccggg tttgcctggt gcaccatcgg cagtatatcc caggccacat
     1441 cgcggatcaa ggtctccacc ggcgtgacct gcccgctggt gcgttaccat ccggcgatcg
     1501 tcgcacaggc tgccgcaacc gcggcaaccc tgatgccggg gcggttcgag ctcggcctcg
     1561 ggacggggga gaacttgaac gagcacatca ccgggagcgt ctttccgacg tcggagccgg
     1621 tgcgcctcga tatgctccgc gaggcggtga gcatcatccg gatgctctgg aagggcgggg
     1681 tgcaggacta ctgtggcgcc tactatgccc tcgacaacgc gcagatctac gaactcccgc
     1741 gggacctccc gccgatccgt atatcggcgc aaggaccgat ggcggcggag gttgccggcg
     1801 atatcggcga tgcgctcatc cacttcgagc agaaaccgga ggagatcatc gaggcgttcc
     1861 gtgcatcggg cggcgagggc aagtcgtgca tgatcgagac cgccgcctgt tacgctggaa
     1921 gcgaggagga ggcgaagcgc acggcatacg aatggttccc catggaagag ttacgaaccg
     1981 caccggttcg gtctgaaaaa aggtggatcg gggtgccggc actcaggcgc cgatcgcttc
     2041 gaacaccggc ggagtgcccg ccacccagat gtcatactgg gcgtccggca cccaccggta
     2101 tccgtcgccg tcggccttaa tcgaccagaa acagtagtgc acggtcgacc ggtcgccggc
     2161 agcattcagg ccgaccacat tgcagaaggg tccggcgaac gtatccgaaa ccttcgttaa
     2221 ggcgatcttc aacttcggtg tctccgccga tgcggtctgc gtcccggtca gggtgagaag
     2281 ccagagtgcg tcgtaggacg ccaggctgta accgtcgggc tgccgcccga gccggttctg
     2341 gatcttctgg aaggtggcct cgtgtgccgc ggcaaggttt tgctgccaga acgtcggacc
     2401 ggtgaagttc gtctgcgccg caaaccgggc ggcgtcgccg ggcgtcgcga gcgttccgag
     2461 gagaacgttg ccgtcgcacc cgtaccaccg gaccccggtg aggttctgag tctccgccgc
     2521 tctcgtcatg atcggcgcta tctcgttgag ggtgactgcg tagacgcaga cgttctcctg
     2581 cccatgcgcc gcgatcgcct tcccgacctg cacgtcgagg tccgccatcg tcccggcata
     2641 gtccttctgg cccggcgtgt acctgacccc gtcataaacc gtgccgtttt gggccttgaa
     2701 tgccgtcgtc gtcaggttcc ggagttcgtc gccccatata tcgccgcgcc ataccggcac
     2761 gaccgctgct attccctcct cctccaggta gtatgccatc gcatccgcct ggtaggtgtc
     2821 cggcgacacg agccggaaga tgttgtcgcc cggaatggca agcgaaggcg ccgtgctcat
     2881 ggtgctgacg atcaggatcc cgtgtgcgtc ggcatacgcc cggacggcct cgagttcggc
     2941 gctgcaaccg ggcccgatga ccatcctgat cccctgcgcg tcgagcgctt tcaacttctc
     3001 aagcgcaacc gcagggtcgg tcgccgtatc ctcgataatc ggccggaccc ggtggtccga
     3061 cccgatcgag gcgaagtagt cgttgatatc gccggcagcc acctcgacgg cggcccgact
     3121 cgccgctccg ctctcggcaa agtcgccggt gagcggcagg aggacgccga cgacgttctc
     3181 ctgcacttcg gcctgctctg tggtcgtggt tccggtacat ccgcaggcga agatgactgc
     3241 ggcaagcgtg atgagcgcga ttgctggtac ctttcgtctc ataaggtttt ttctgcagga
     3301 gtgggattta gcctttctgt ttttgcatcc ggcagtgctg cgaatatcgg atgattcatc
     3361 gccggattgc cgttcggggt gccagagaaa gaacggtgct gcccggaagg cagcacgccg
     3421 ttccgctcat aggagcgccg tcttcaggta ctctccctct gcggcgacgg cccgctcccg
     3481 ccagtccgcg acaacccccg catacgcatc gaagcgggga gagacgaaga gcatggtgcc
     3541 gtcgtcctcg agcgtccgga cctcccttcc tcccctgaac gtcccgttga tctcgtttgc
     3601 caccgcgcga tagacgatcg catcgatctg cttcaccacc gatgcgataa cgaggttcgg
     3661 ggcgaggtag gactggtcgg tatcctcgcc gatgaggtag atatccccgg tctcccgtgc
     3721 agtatccact atcccgatgt ttgaagcgcc tgcaatcatg aggatgacgt cgacgccttc
     3781 ggcgcggagg tcccgggcga gtgcacccgc ccgctccggc atgccgaacc cttcggcgtt
     3841 ctcgccgaca taacgcaccg tgacgtctac cgacggatcg tatgccgccg caccttcagt
     3901 aaaccccctg acgaacgggt cgatgacggg gaccggtgcg ccggcgatcg cgccaatctt
     3961 tcccgtcctc gtgatattcg ccgccatcac gcccgcgagg taggatgcgc cgtaagggac
     4021 catcatcacg tcgcatgcgt tcggaagacc aaactccgcc tgttcgaggg tgacgaaacg
     4081 gatgtccggg tttgcctctg cccacgcccg ggaggcgtcg gtgaactcga acccctcggt
     4141 gatgacgagg tcgggcttct cggtgaagtt ctgtagagag aacacggcgt cgaggagcgg
     4201 aatgtcggcg taggtgcact ctctcttcgt gaacgagaac gactcctgcg cacggaaaag
     4261 accccggtac gcggagtcgg caaacgaccg atcgcctttt gccatcgggt agaccatcca
     4321 gacgaccggg cgaacgccct cgccggcggc cggatcgtac gagaggccgg gcagccccgg
     4381 ttctccgccc gcagaagaga cgggggtgct ctcacagaac gccccgccct cgacagtcca
     4441 gcgggtatag taccctgcga gcggcccttc ggatgagtgc tcgccgaacc ggagagggcc
     4501 cgaagcgccg gtgacggccg ggtgagcgcc cgcacggatc atcgccgcgg cctcgcctgc
     4561 ttcttcggga tcccagcctt tggcgatgcc gtcgccggag acgacccagc ggagcgatgc
     4621 ggcgagcggt tcacccgggg ccgcctcctg ccgtgccgcg gtatagacgg cgaggaggag
     4681 ggcatcgtag gtctgtgcgg caaacgacgg cggggcctcg ccgaacaact cctcgtaggc
     4741 gattgcgtac ccggtggtgg ggtcggagga gagcgctgtt ccctggatac cctccgcccg
     4801 ctcgccgagg cgctcaagca ggtagggcga acgggcggca tcggtcagga agaggccggc
     4861 ggtcgagccc gcccgggaaa gtgcatccgc tatccggacg gcttcttccg ggtacacggc
     4921 ggcaacgacc gtcccgggtt cctcctcccc gatccgcatg gcgatctctt cgaaatcgct
     4981 cgatgcatcg acggataccg cgccggttat ccggatccgg ttcgccgcgg ccgctgccgg
     5041 ggcgaaccgt gcaaacgtct ccccgtaggg tgcgttcgcg acgatgagag agacgttccg
     5101 gacgccggtc tccgaaagga tcctgaacat cgcctccacc tgccggtcgt cagtggggca
     5161 ggttctccag aagagactgt tgtcatcgat ctctcccgtg acgctcgggg atatgaggag
     5221 tttaccccgc cccgtaacga gaggggcgat ctctatgagt tccgtgcttg tcgcagggcc
     5281 gatgacgata gcgatatcgt cgcgcccggc gagttcctct gcgtacgcgg atatgttgcc
     5341 ggcactcgtg tcccggtaga cgagttcgat cgtgcggtcg ccctgccggt tcatcgcacc
     5401 cgcagcccag gtgagtccgt ccagcgaatg cacggcaacc ggcccgctcc cgggcagaag
     5461 gacgccgata cggatgggat cggcggatgt cgtcgtagcg atgcagcctg cagaagagat
     5521 ccctataatg gcgatgcaga tgagtgcaag gaaccatatc ctgtcggctt ccgatagcgg
     5581 gggtcgtttg atcatcgtaa tccttcgtcc gggcgcctgt ggtcgatatc ggcagccgat
     5641 cgttgtattc ggcggtgagt cagatatgtt ttgttattga ttctccggcg ccggacgggg
     5701 cgacggcagg caggcatatc atcaatcgcg cgtacagccc tttacgcggg cggttcatgg
     5761 gccatgaccc cgataccggt ctgttggttc cctcatacgg gcgagagggc ggcgatccgt
     5821 cgcctggtcc ctgcagacca accgtcagcc ccgggaaggt tctcccgatc gcgggatggc
     5881 ggctcgttcg aaacaattat gccgggcggc ggatagcagg ctccgatacc cacccccgga
     5941 gggagagatg aacaagaacg catgtgtccc ggccgttcgc gacctcatgg cggagtttgc
     6001 cggccggaca gggcttgacc ccccgctcgc caatccgcgg cgctacctct ggaccgatgc
     6061 gtatgcggtc tgcaactaca tcgaactcta ccgccggacg gaggaagaga cctatcggga
     6121 cctcgccctc cgcctcgtta gtcaggtgca ccacaccctc ggccggcacc gcgccgacga
     6181 ccggagaacg ggctggatca gcggcctctc cgacggggag ggcgaagtac acccgacgat
     6241 cgggggcctc cgtatcggca aaccgctccc cgaacgccgg agcggcgagc cgttcgacga
     6301 gcggctcgag tgggagcggg acgggcagta ttaccactac ctgaccagat ggatgcacgc
     6361 gctcaaccgg gtgagccggg cgaccaacga tccggcctac atcaggtggg cggtggaact
     6421 tgcacgagcg gcccacgccg cgttcgccta cctcccccct tccggcggca agaagaggat
     6481 gtactggaag atgagcatcg acctctcccg gccgctcgtt cccgccatgg gccagcacga
     6541 ccccctcgac gggttcgtca cctacagcga actgcaggct gccgcaagac cgttgcacct
     6601 cgacctctcg cccgagatcg ccgacatggc cggcatctgc cgcgggactg tccaggccac
     6661 ggacgacccg ctcggtatcg gcggcctcct ctttgacgcc gggaggatag cgcagtcgat
     6721 ggatcggagt gagactgacc gtgccgacct gctgtgttcc gtcctcgacg ccgctcttgc
     6781 cggccttgcg gcatttgcga gaggtggcag cctccgctac cccgtggagt accgccttgc
     6841 cttccgcgaa ctcggtcttg cgatcggcct tacgggtgtt acagggcgga tccgggagga
     6901 cccggagatc ttcgggcgcg gagcgctgga acggcgggcg gaaactctca tgggttatgc
     6961 gccgcttgca gatgcaatcg tagagttctg ggcggatgag aggaaccggg aagccggcaa
     7021 ccgggccggg aaccccgaga tcaacatggt gatgctcgcc actgctcttg ctcccgggga
     7081 gttcctggcg gtctctctcc gggacaacca tacgctttta tgccggggcg gcgaggcggg
     7141 ctcccggcga atgcctattc ggtgaactcc gggatcgtgc tcgggagccc gacccgcatg
     7201 aacacccggg caaaggcctg cgagagctgc tcgcggtagg gaggacagag cctgacccgg
     7261 tatgggagcc cggcggcaag gcgttgcacg aactcaaggg gaaggctgcg cgggtttttc
     7321 agatcgacga agatatagcc cgattcgtgc ccgggaatgg tgcatcgatc caggaggtga
     7381 tacgcgatca tctcgccctt cctgagcttg ttttttgtcc tgacgactgc tttctttgaa
     7441 tcggagcggg caatctctgt tatatgttcg tccagggaga atatcgagga gagaatgaca
     7501 gagtcgacct tctgctcgat aaggtcgcac gactgggaca tgacgatgac atcgtactct
     7561 tcgcaatcga cccgcgagtc gccacgcaga tcgccggcgg gagaaataaa gggaacggag
     7621 tggaggatat cgccctgggc gagaggttct gccgcggtca cgagagtgta ccacggatac
     7681 tcgccttcca tatgactttc ccgactacag gtcgatctcg gtgtgatcat agtttgttat
     7741 ccgccctctt ctcctggtga cgacctgtac tatcagcggc attcttctct tcgggggcaa
     7801 ccggacgatg tgtgcatctt ccgacccggg gatctcctca tctccggcac cccgttcttt
     7861 gaggtaggtg gtggaactgc tcttcagggc ctggtgtatg accgaggagc tctcataagc
     7921 gagagatagc tcgcagaccc ctgatctggt gcagtgatgt gcgcctgccg gacgggcgag
     7981 gaccatggtc cgggtggagc tgacctcgcc gaagtggtcg tatggcctga ctatctgtgt
     8041 tgacggcgaa tacccggcgg gccgccggcc ggcagggcgc tctatcagcg acggcatgca
     8101 aactcctcca ggagatcccc ttcgatcatc gtaaagaacc aggtatgggt cagggcgtgg
     8161 gcttccgaga cccaggacag gatctcatcc tttgttctcg gtccgtctgt ctccggggac
     8221 tggacgatgg tctctccgat caccgaatcc ctccccttta ctctccccct tgaaaaacca
     8281 acgtggagat acccgggagg tctctgcgaa gggaaagagt acttgaagtc gacgccgatc
     8341 ggtactggtg agacgccgat cgcctcgaag aaggcagggg atatctggtt ttgtactccg
     8401 agttttgctg ataaaaattc agcaacattc tctttctcga agtcaaaagg gtaggcatcc
     8461 agatacctga ggacaacctc gttggtggca aggagggagg tttcgggata ctcctggaag
     8521 agcgcatcaa ggacgccttc tatccggcgt tggaagtcct gccattcgta cgactcgata
     8581 tcgttgagag ttacgatgcc cggcccgacc tgcaccagag gccagctgcc cttcgccctc
     8641 ctgaaccggt gctgaacgat gtaacctgat atctcatcgg ggatatcgga tgtggggagt
     8701 tcctcataca ccgggtactc ctcgcccagt gcagacgata gccttccggg gaagatcttt
     8761 gcatgtggat cgaagcgcac cccatcttct ctctcctgga gatcccatcg tatctcgaat
     8821 atgacctcaa tgaggggtgg gttctgtaat ttagccgtac ccatatgtgc aaccgcctgc
     8881 cggatatctt ctctcaaagg gtgaggggca cagcagttaa ggtttgtgat cctggtccgg
     8941 gaggagccac gcgcgacgac gtgcaaccgg gacaaccata cgcttttatg ccggggcggc
     9001 gaggcaggtt catggcaaca cccagactta tggagtattt caacaggcag ccgcggatcg
     9061 gcatcctcag caccgcgaac agagacggaa aggtcgatgc ggccgtcttc ggttcgccgc
     9121 agatgaccga cgagaagacc gtcgtcatgg ccctcggtga gaaccgcacg ttcgaatacc
     9181 tccgggagaa cccgagtgcc gtcttcacca tcgtggagag cggaaagacg atcgcggatt
     9241 ggaaagggct cagggtctac ctgtggatga aggagtatgc aacgtcgggg gagatgctgg
     9301 agacctataa gaaagaggtt gcccgggccg tgggcgatga ggcggcgaag atggtccatg
     9361 ccgtggtgac gttcgagatc accgaagtcc gtcccctcgt cgatatgggc cagggctggg
     9421 agggttccct ctgacggggc aacccgcacg gtctactccc gcgacctctg gtagaggaat
     9481 atcccggccg cctggaagat gacgatcgcc agcaccaggg cggcgacatc gatgaccggg
     9541 ccaaagaggg tcgtcccgtc gaacgcaaag agcatcaggt cgtgcgcgta ggtgaggggc
     9601 gagaagaccg caatgctctg cccccatccc ggcatgctcg cgagggggat gaagaccccg
     9661 ctgatgaaga ggaggggcag gcggacgagg ttcaagagcg acatcacctc gcccacgttc
     9721 tccgtgcggt aggcggcaaa gagggtgccc atcgtcgaga agcagaggga ggtgagcgcg
     9781 aacccaagca ggagcgccgc gacgttcatg accccggcgt tgaggacgat cagggcgatg
     9841 acggtcgtcg cgacgccgat cgccgatgcg aagagggcgc cgctcaggct tgccccgagg
     9901 acgatcgccc ggatcgatat cggggcggag aggaggcggt cgaacgtctt cgtcctccgc
     9961 tcgatcggta tggagaccgg ttcgatcgag gaggcgctga agaggagggt gacggcgagg
    10021 agaccgggga tcagggttcc ggccgcggcc tccctcccta cggcgaacgc gaggaacatg
    10081 acgaacggga agagcaggct ggataccagg atggagggct tgatgtagta gatcttcatg
    10141 tccttgcgcg caatcgccca tgcggcgccg atgtcacggg agactccctc aaaccatccc
    10201 ggcatcggtc tctctcacct cccccttcgt gccgacggag agcccggtga ggcggatgaa
    10261 gacctcctcc aggctcggcc cgcgggtggt gacgctgata gggcgcatat tctgctcctg
    10321 tgtaaacgtg cacagagagc tcaggacctc cggggggtcc gcggtgtaga gccggaattt
    10381 gtccccctcc ttcctgacgt cgttgacctg cgggagcgtc ctgagtgctt caagaaggcc
    10441 gggccggacg gcatcgaagg atatctcgat cgactgaagg ctctcgaagg tctgcttcaa
    10501 cttctccggc gcatcgatcg ccgcgatcgt tccccggttg atgatggcga cccggtcgca
    10561 ggagacgttc gcctcctcga tgtcatgcgt cgtcaggaag atggtgacgt tcttccgggc
    10621 aaggtcctgg acgacgtccc tgatgagccg ccggctctgg acatcgagcc ctgacgtggg
    10681 ctcgtcgagg aagaggactc ccggattgtt cacgagcccc atggcaagcg tcaggcggcg
    10741 tttcatcccc ttcgagaacc ctcccgcggg ctcgtggcac cgctcgtaca ggccgaaggt
    10801 atcgaggagg tcccgggcgt tcctctcgat ctcggccttc ttgagatgat agagccggcc
    10861 ggtgaagatg aggttgtccc aggcggagag ctcgtcgtag atgttcgacg tctcggggac
    10921 gacgcccatc aggcgtttcg cggccagggt atcgcgggcg atgtcgtggc cgtggatcag
    10981 ggcacgaccg ctcgtgatct tggtcatgcc ggtgagcatc ctgatggtgg tggtcttgcc
    11041 cgccccgttc gggccgagaa acccgaacgt ctcgcccatc ttcaccgcaa aatcgatgcc
    11101 tttcacggcg acgttgtcac cgaaccgttt ggtgagtccg atcacctcga tcgcgtccat
    11161 ggccccagct caccatatgt ttctttccgg aggataaaaa aggccctgaa gtacctctgc
    11221 tcccgcctta gtcagagaga atggagaata tccgccgcag gttctcgttg agtggttttt
    11281 tcttcttcca ggtctcttca agcggcgtat acgcgagggc gcccccgacc tcccctacca
    11341 tgcactcgct ccgcccctca agcagcgcct cgacggcggc gacgccgagg ctactcccga
    11401 gcacccggtc gcgaagcgtc ggcggcccgc cccgctggag gtggccgagg ataacgaccc
    11461 gggaatcgag atccaggtgt tttttgacct ccccggctat ctggaaggag acacccggcg
    11521 tctttgcctc tgcgaccacc acgatggcgc tcttcttccc gatatcgaac cctttgcgta
    11581 tgcgctcgct catcggggcg atcgacgtct cttcttccgg tatgatcagt tcctcggcgc
    11641 cgcctgcgat gccgctctcg agtgccagga aaccggccgt ccgccccata acctcaagga
    11701 agaagaggcg ctcgtgggac cgggccgtgt cgcggatgcg gtcgatcgcc tcaaccgcac
    11761 aatttgccgc cgtatcgaac ccgatgcagt aatcggtccc gtagacgtcg ttgtcgatgc
    11821 tccccggcac ccccacgagc ggcgccgtat ccgcgtcggc ggcaaccagg cttgcgccgt
    11881 ggaacgtccc gtcgcccccg atcaggatca cgccgtcaag ccccatcttc ccgatggctt
    11941 ccgccacccg gagcctcccc tcccgcgtgt agagatcggg actccgcgag gtctcgagga
    12001 tggtgccgcc ctggtggatg atgttccgga tcgccgcccg atcgagcggc accgtatcgg
    12061 tggcgatgag accggtgtag ccccggcgta taccgacgac ggtgaggttg tttgcaagcg
    12121 ccgcccgcac cgctgcccgg atgcaggcgt tcatgcccgg ggcgtcgccg ccgcttgtca
    12181 ggatgccgat ccgcttcatg atccttccgc atcctcgagc gtttccacgc ccggaagcga
    12241 ctttcccgcg agcacggtga gcgccgcccc gccgcccgtc gagacatggg tcatcttatc
    12301 ggcaagcccg aaccgtgcca ctgcgtccgc ggtcgaaccg ccgccgacga cggtggtgcc
    12361 ggagaggttg gcgagggtcg cggcaatccg gcgcgttcct tccgcaaact cggggatctc
    12421 aaagaccccc atcgggccgt tccagacggc ggtccggcag ccttccagtt cgcggcagaa
    12481 ctcgcccgcc gtctcgggtc cgatatcggc gatgacccaa tcgtccggta tctttgcgac
    12541 gggcacaacc tgtgtcgggg caccggcctc aatcctctcc gtgacgacgg catcccgggg
    12601 caggaggagg cggacgccga gttccatggc ctttgcggag agctgccgga cgtcgtccag
    12661 gcggtcggtc tcgacgtgcg acctgccggt cccgtagccg cggctcgcaa ggaacgttgc
    12721 cgccatgccg ccgccgatga tgaggaggtt taccctcggc atgatgttat cgaggacacc
    12781 cagtttgtcg ctcaccttgg ccccgccgat gacggcagca aacggtcgtt cgggatctct
    12841 caggatgcgc gtgaacgtat ccacctcttt tgcgagcaaa agcccggcca ccgccggcag
    12901 gtgctccggg acacccacga tcgacgcgtg tgcccggtgg gaggccccga aggcgtcgtt
    12961 gacgtagata tccgcgaggc tggcgagttc ctccgcgaag gccggatcgt ccgtcttctc
    13021 ctccgggtgg aacctgaggt tctcgaggag gacgacatcc ccgccgctca tcgcggccac
    13081 tgcgctctcc acctcggggc cgatgcaatc ccggagcgcg gtgaccggcc ggccgaggag
    13141 cacggagagg cggttcgcca cgagggcgag acgtagacgc tccacgacca cgccctgcgg
    13201 ccggtccagg tgggagcaga ggatgacccg tgcatcccgt tcgctgaggt agcgtatcgt
    13261 cggcaggctt gcccggatac gggtgtcgtc ttcgatggcg ccgtcctcgc cgagcggcac
    13321 gttgaagtcg gcgcggacga ggactcgttt tcctgtcacg tcgatatccc ggacggtctt
    13381 cttccttctt ggtatcgcct tctgcatacc ggcacacctc cggaacggcg gaaaggggcc
    13441 gggcggttcc gcctcccggt ccgtttttcg gccgttccct gtagaatcac ggtgctcccc
    13501 tgcatgcatg cgccggcata tatactgttc cctcccggga tccctgtttg ctgccgggcg
    13561 ggccggtgcc cctgcggtcg tcgccggggg tcacgttctg tcaacatgcc ggtcccgcac
    13621 ctgacccggg accggttggt cccggcagac tcagcgcccg gagtggtgct cccagtaggg
    13681 tggatacccg tagtggctgt acatgcgggt gagccactcg cgctcggccg tcgtcggcca
    13741 gttgttctta tcaaaacccg gtgcgttctc cagctgatct ctgggcacat cgaggacaaa
    13801 gtagtcatcg gccgcattgt actggagtgc cgcccagggg atggcgaaga gcttatcgcc
    13861 gaggcccatg atgccgccga acgagagggc ggcgtaagcg atgcagccct cgttgatatc
    13921 gatcatgacg tctttgatgc tcccgagatc ttcgccccgg ggatttttga ccgagctatc
    13981 cacaacgtcc ttcccccgtg caatagttcg cgccttcgct tgcgtggcag ttcctctcat
    14041 aggtgtctca tctcctggtt tgtggaacac gctctgttcc acggcaagga agtcactaca
    14101 ggtcgtgcag tatttaatcg ttgtgacaat atgttgggtc ctgtataaaa tcgggtgccg
    14161 gcgtttttcg gcggttatcg cttcattggc cggccggttg agcatcctgc ggatctgcga
    14221 gagctccctc aaaccgtctc cacgcccggg gaggaacgag gatctcaaac cgggctcccc
    14281 ggcccgccgt tccggtctcg cgtatcgcaa tatcggttac agagaggaca tcccgggaaa
    14341 acgccagccc gaacccggtg ttcttcccgt acccatagca aaagatcctc tccttctcgt
    14401 cgtccgggac ccccaccccg tcgtcctcga acgccacgac gatcatccca tcagcccctt
    14461 cggcggtcgt tatgcagatc cgggtgattc ctcctccgtg ccggagcgca ttctcaagca
    14521 ggttgtacac gactttcggc gagagcgggt cggcgtagac ctccaccggg gctacccgga
    14581 cggcaatctc gactccaggg tggtgtatgc tgccgacggc tccttcgatc atcggcccga
    14641 gcggttgcca gaccgactgg taggcgccga gcatctggca ctcgcgcgag aacgcaaggt
    14701 gctttgcgat atcctccacg atatcgaccg atcgggtgag gtacgtgccg gcgtcgggat
    14761 gcgtccggtc ctcggagagg aggttcaggt accacgcaag gccggtgata tcgttgccga
    14821 tgtcgtggcc ggtcacctgg gagaggaggg agagcttctg gttggccgtc cggagggcgg
    14881 tctccgccgc cctcctgcgg gtgatgtctc tgatgatcag gacgacgccg gcagactccc
    14941 catccgggtt tcggacagga gccccggaga tgctgatggt gcggccctcc ctgccgccag
    15001 gcgtcgcctc gagatcggag aacctcccct gctccgcgat ggcggcccgg atcgtgtcgc
    15061 acgtcgtatc ggggatgaac ctcctgaccg gctgtccggg caggtccgct tcggcaacgc
    15121 cgaagatctc tgcggccgat gcgtttgccg tgacgacccg cccgtccata tcggcaagga
    15181 gcaggccgtc gggcatcacc ttgaggatat ccacgaccgc agtctggggg ctcagggcaa
    15241 acagcccatg ccggtgaatg gcgtaggaga tgaggaggga gagcaggacg aacccgatga
    15301 agacgacgtt gacggtataa actcctagag ctggaaggac cagccctgag atagcgccaa
    15361 aaccggcagc ggttacaatg ccggtgagta tgagctggtg ctggcgcctc tctctcctcg
    15421 tgcgcaccct ccgcaaagac gagatgccga cgtataatcc ccagactatc gccagggtaa
    15481 tataggctct cgaggccagg taggcagggc ttgcggctgc cggctggtag atatagccca
    15541 ttccgggctg gaataccacc gtatagatca ggttggtgga gaaaccaatc agggcaatga
    15601 ggagcgcagg gagatagaga attacaagga ggatccagcg cttatcctcc cgtgcgaagg
    15661 ggtggttggt gaagacgagc gtgaagtgga cggttaacgc aatgacgaaa ctccagaacg
    15721 agctgatcct cagccagaac tgatagccgt cgtagctggt cgactgccag atgaagaact
    15781 ccccgagagc ccagcaggtc gcggagaggg ctaatgcaaa gaagaggcgg ttgatggtcg
    15841 atgaagggtt ctgtccaaga acgtatgccc cgaggctata ggtgatgagc gccgagacgg
    15901 cacaagaacc gaccagaatg ggaaagagga tcgtgaggag ttcagacatg gccaatcaca
    15961 ccataacatc atccgaattc tcccgggcag atccgatccc catcctcgca ggaatctgtc
    16021 acagggccaa cgcaggatta tttgacaaac ccctatagta acctttcttt ctgttccggg
    16081 cgtgggaggt gagatagatg aaaatggacc tttccgaccg catgaaaggc acaccagccc
    16141 atgcaggatc agttcctccg ggccgatcac cgcgatcggc gcggataacc cgtcagatcg
    16201 caccaccggt gctccttccg ccgcggatac gcttaatatc ggactcgtgc tacgattatc
    16261 gaggatgcaa agtgagcggc agggagtatg tccacgggta taccgggcgg gaagcggagc
    16321 ggttgcgcga ccaggcggag accctgaccg ggctcctcca cggcgacacc cgctacccgg
    16381 cggggtcgga ggtgctcgag gccggctgtg ggaccggcgc ccagacggtg atcctggcac
    16441 gaaacagccc ggaggcgcgg ataacatcga tcgatatatc ggcagcgtcg cttgacgcgg
    16501 cccgggcacg tgttcttgca gaggggatga agaacgtcac cttccgggcc ggggatatat
    16561 acgacctccc ctgccctccg gggagtttcg accacatctt catctgtttc gtcctcgaac
    16621 acctggcgga gcccctccgt gctctcgagc gccttcgccc ccttctccgg gcgggcggca
    16681 cgatcacggt gatcgaggga gatcacgggt cggcatactt ccacccggac agcccccgcg
    16741 cccgccgggc tatcgggtgt ctcgtcgacc tccagcggga gatgggcggc aacgccctga
    16801 tcgggagaga actctacccg ctcgtcaccg gtgcgggata ccgcgacgtc cgcgtctccc
    16861 cgcggacggt ctacgcggac gcgacccggc cggaccttgt ccaggggttc acacggctga
    16921 cgttcaccgc catggtcgag ggcgtcgggg aagatgcggt gggcaggggg atgatgagcc
    16981 gggaagactg ggagcagggt atacgcgacc tttaccgcac cgcggaaccg gacggagtct
    17041 tctgctatac cttcttcaag gcgaccggga gaagat
//
LOCUS       DAOSEE010000035        16792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1393_length_16792_cov_257.296290, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000035 DAOSEE010000000
VERSION     DAOSEE010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 16792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16792
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1393_length_16792_cov_257.296290"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PLI76_04195"
     CDS             <1..239
                     /locus_tag="PLI76_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOI61265.1"
                     /translation="YFEYSACGRPILSTPIPDMLRMGGGSIAVYRDKEEFIEKVDELA
                     GNPGRCPAEVEGHSWKKKAEAFEKVFARLIGKVW"
     gene            322..2967
                     /locus_tag="PLI76_04200"
     CDS             322..2967
                     /locus_tag="PLI76_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004576 - oligosaccharyl transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006487 - protein N-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharyl transferase, archaeosortase A
                     system-associated"
                     /protein_id="HOI61266.1"
                     /translation="MAHVDLDKYRPYIIIGVIVLLTLLPLWVRGFIPAADMVTPDGVN
                     LLGNDPWYNVRQVEQTVANFPGYAWFDAMTLYPSGDVVYWGPLFIQIISALCVLVGAT
                     TRPEIMVVASWVPPLMGAAMVPVTYLLAKRLTNWKTGLIAAGLIAVVSGSFAHRSLFA
                     FVDHHIAETLFSTIFVLAYVVALLASRDISLSRRSSEVLKMPAITAAFAGVAYLIGFF
                     NMPTMILLALIVAVFTLVQFVLDFVQDRPSDYLVLVNAVTFGVVIIGTAAFGFPHPNL
                     DLSRYTAGHVVACAGLIGGTLVLYGLSVYLRGRPKYYFPAALAGLGVVAVAVLSIALP
                     DIYNLLISSLFAFFGEAPVTTTVQEARAWSFDAAWATFHWGLVLAAGGAATLIWWSRK
                     KVNPAHVFILVWTAIILASTAAHVRYEYYLAANIALLAAIFAGAILDVTYKDVTRLFG
                     AKSSTAPPDAEVARKEEKPARKGKKGARAPDARKTKVSSKDQPDYIKVGAFAAVVVVT
                     LVFAGTSLQTNFAYAASAQYSGINSQWMEALEWMGTHTPDPGVDYHAIYDQDTFTYPE
                     ESYGVMSWWDYGHWITFIAKRIPNNNPFQHGVAGPNGSATYFVSTSEEEANRVLDNVS
                     TRYVITDIDLDTGKFHAPATWANPDVGRDRFEPTFFIPVSAGSTSYQAMTFYNQQYYL
                     TMVSRLHNFDGSMTDPTSQVIYAEYRDADAANASLPVITRTEQMNATAAAAAVEAYNK
                     NAPAGSHATLLNMYYQYRGDAILQPVEQVPALQHYRLVHESPQNVFGNVGAGGPDLKS
                     VKIFEYVPGARIKGDGIIEVPVVTNAGRAFTYRQESVNGTFIVPYATSGWSGEVKTTG
                     PYRIAGTGQTFDVTEEDIRQGRTIN"
     gene            2974..3942
                     /locus_tag="PLI76_04205"
     CDS             2974..3942
                     /locus_tag="PLI76_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOI61267.1"
                     /translation="MRASVIYGDIFTRHDMEAHPESGSRLLGALAGVPENVRWHAPVR
                     ATEQDLERVHRPQYIRWVQEIARGPCFLDPNTYVTCHSFETALYAAGSTFAAVERALD
                     GEHQFALVRPPGHHAEPDQAMGFCIFNNAAVAAAKALLSVDRVAIVDWDLHHGNGTQT
                     AFYGSDRVLFCSVHEADSFPKTGWVDEIGTGAGRGYTLNAPLAAGSTIADYQHVFREI
                     FVPALVRFRPDVLIVSAGQDALSDDEHGRMNLEPEDYTALTGMLIDGTDLPLALTLEG
                     GYGPSHGKAIAAIFRALSGKRHTPAERPLHRTTERVVDILKKVRFC"
     gene            complement(4354..5529)
                     /locus_tag="PLI76_04210"
     CDS             complement(4354..5529)
                     /locus_tag="PLI76_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-related McrA-glycine thioamidation protein"
                     /protein_id="HOI61268.1"
                     /translation="MAIMIHPVEKLYFDGTHRSRSPEETYAAVEPLMAEIGVTEIVDV
                     TPLDRLGIPVFTAVRPGAARGAARVHAGKGKEPIHARVSAMMEALERYCAEYRGDRME
                     YATYEEIGPGRAVHPEDLFVPRKLEQGEKLHWTPVWDILNDEEAYVPSNAVFHPYDSL
                     GMAVPLFRSDSNGLASGNVMEEAILHALFEVIERDALSLAEQRHDLGHRLTIGNDCAA
                     REVLDRFEENGIEIHLWLLDGKTGIPTVAAAADDTVTRDPAMIVIGSGTHACPEIAAL
                     RALTEVAQSRGSYLQGGRTDPQREMVIRKAGYERLKRINRMWFADAEAVDIRDIPDVS
                     TNRFDLDIERALQEISPYADRVCVCDLSRTPVPVVRVVVPGFEVSYMDPDRKRPAQG"
     gene            5586..5876
                     /locus_tag="PLI76_04215"
     CDS             5586..5876
                     /locus_tag="PLI76_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2098 domain-containing protein"
                     /protein_id="HOI61269.1"
                     /translation="MSTDDVVVGAIVRYPRTGTTGKVLRVEDIDGRRYAELDSTGLYY
                     RVDELVGADRTTGPVEREGHSLEDYLAEHMEFKEQLEQVWEKGIDQSCEGGG"
     gene            complement(5955..6377)
                     /gene="nikR"
                     /locus_tag="PLI76_04220"
     CDS             complement(5955..6377)
                     /gene="nikR"
                     /locus_tag="PLI76_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HOI61270.1"
                     /translation="MSGDTELSRIGISLPKNLLDKFDEILSLRGYSSRSEGIRDSIRS
                     YITHYQWISDVKGERQGVITMVYDHDQRGLLQTLTEIQHQYAHIIQASLHSHVTHNRC
                     LEVILIRGDGAVLKDITERLMAQKGVEAVKLTTIPIEG"
     gene            6590..7825
                     /locus_tag="PLI76_04225"
     CDS             6590..7825
                     /locus_tag="PLI76_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     gamma"
                     /protein_id="HOI61271.1"
                     /translation="MRDAFIPGVNIGLVGHVDHGKTTLVSALTGTWTDRHSEEIKRGI
                     SIRLGYADTTFYRCERCEGAEAYTSRPECPSCGGKAVPFRTVSFVDAPGHETLMATML
                     SGSALMDGAMLVIAANEVCPQPQTKEHLMALELIGIKKIVIVQNKIDVVTQAEALEHY
                     KQIKRFIKGTIAESAPIIPVSAQKGVNIGALIQTLDSIVPEPARNPDIDPVLFIARSF
                     DINKPGCSWRDVKGGVIGGSLIRGILHEGDDIEIRPGRQVQVENRMKWEPVETKITSI
                     NAGKTRVTEAAPGGLLGVATKLDPALTKSDALAGQVAGLVGKLPPVWERLKFDVTLMD
                     RVVGADSEQIIEPLKHKEPLMLSVGTAVTVGVIVNTKKNQVEVQLKRAVCAEVGARIA
                     ISRQVGGRWRLIGMGVLVE"
     gene            7822..8202
                     /locus_tag="PLI76_04230"
     CDS             7822..8202
                     /locus_tag="PLI76_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HOI61272.1"
                     /translation="MRVLLDTNALLMPAQFGVDLYDELLALFGDFEPIALEEVVGELS
                     GLARGRGRDAAAARVGLALVRRSTVVPSGSTAEGVDNRVIEYARREGCTVVTNDRELR
                     NALLREGVDVVSMRRQKTLELLRG"
     gene            8206..8775
                     /locus_tag="PLI76_04235"
     CDS             8206..8775
                     /locus_tag="PLI76_04235"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HOI61273.1"
                     /translation="MYYKMTLEDKVRVPPHRLGEDLGAVILNVLQEQLEGSIAKEIGI
                     FIAVTKIIDIGEGELIPGDGAVYYDVRFEAAVLRLGLQEVIEGEVVETTSFGAFVSLG
                     PIDAMLHVSQISDEYINYDEKNGRLICQDSKRYIAVGDAIRARVVALSLNEREPRESK
                     IGLTMRQPGLGTSVWLEEDLEEEKQKGAA"
     gene            8777..8977
                     /gene="spt4"
                     /locus_tag="PLI76_04240"
     CDS             8777..8977
                     /gene="spt4"
                     /locus_tag="PLI76_04240"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003711 - transcription elongation factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor subunit Spt4"
                     /protein_id="HOI61274.1"
                     /translation="MVVRKKVVKVCRECHRVVEGEACVICGTANLSEDWAGYVVIIDP
                     EHSDIAKKMKITLPGRYALKVR"
     gene            8977..9465
                     /locus_tag="PLI76_04245"
     CDS             8977..9465
                     /locus_tag="PLI76_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-dependent dephospho-CoA kinase family
                     protein"
                     /protein_id="HOI61275.1"
                     /translation="MLRLPEAHRDLFKKPFGTLYGSIGELLPRLSGRAVYAVGDVVTH
                     NLLDAGVIPDVAIIDGYTMRLPCTRLPLFRARRRLTAKNPAGTITDELAGAIADLVRG
                     PPGVLFVDGEEDLAVIPLVLAAPDGAAILYGQPGEGVVLRIVDEPAKREARFMLTCFV
                     RE"
     gene            9527..9820
                     /locus_tag="PLI76_04250"
     CDS             9527..9820
                     /locus_tag="PLI76_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S24e"
                     /protein_id="HOI61276.1"
                     /translation="MDFEITRDVRNEVLSRRELTFTLTFDGPTPSRKSIQEKLAAMLN
                     KNENLVVLDLERTRFGAMELSGRARIYDDEERKKSTERGYLLKRGEPKAESEA"
     gene            9826..9981
                     /locus_tag="PLI76_04255"
     CDS             9826..9981
                     /locus_tag="PLI76_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S27ae"
                     /protein_id="HOI61277.1"
                     /translation="MAVKRHECYELKGDKVVLQKRHCPRCGPGVLMAEHKDRVACGRC
                     GYTEFRK"
     gene            10063..11649
                     /locus_tag="PLI76_04260"
     CDS             10063..11649
                     /locus_tag="PLI76_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional N(6)-L-threonylcarbamoyladenine
                     synthase/serine/threonine protein kinase"
                     /protein_id="HOI61278.1"
                     /translation="MSDTRSEDGLVLGLEGTAWNLSAALFGEGLVALHSSPYIPQKGG
                     IHPREAAQHHASVMKEVVSRVLTEPERIRAIAFSQGPGLGPSLRTVATAARALAIALG
                     VPLVGVNHCVAHIEIGRWATGFADPIVLYASGANTQVLGFLNGRYRIFGETLDIGLGN
                     ALDKFARSHDLPHPGGPAIERLAREGNYIELPYTVKGMDLAFSGLVSAAQESTAPLED
                     VCAGLQETAFAMCVEVTERALAHAGKDEVLLVGGVGANARLQEMLRTMCEDRGASFAV
                     PERTYLGDNGAMIAYTGKIMLEHGVTLPIEESQIRPGYRADEVEVAWWIGPGEIFAAG
                     PLPEGGIARGAEAVVEIREGDVVKRRTGKRYRNPGLDRRLIAERTRAEARLIAAARRA
                     GVPTPVIRDVTADTIVMERVEGDVLKYVTSAENVRLAGKAVGRLHGAGIVHGDLTTSN
                     MIVRGDQCVLIDFGLASTSPEVESRGVDLHVLFQTLESTTENYGELREAFVAGYAETF
                     PGADEVLSREHEVELRGRYL"
     gene            11646..12194
                     /gene="rdgB"
                     /locus_tag="PLI76_04265"
     CDS             11646..12194
                     /gene="rdgB"
                     /locus_tag="PLI76_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOI61279.1"
                     /translation="MRLAVVTSNANKAREAAAYFAGVLEVEHVALECPEFRHVDIGEI
                     ARGKAEFAYGVLSRPLIVDDTGLFIDALDGFPGPCAAYVHDTIGNAGVLKLMEGVENR
                     GARFETAIAFAREDGIRIFRGILPGTIVAPRGIGGFGYDPIFASGEQTLAEMPLAEKN
                     RISHRARALEAFRTWITQEGSS"
     gene            12253..12399
                     /locus_tag="PLI76_04270"
     CDS             12253..12399
                     /locus_tag="PLI76_04270"
                     /inference="COORDINATES: protein motif:HMM:NF003161.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L40e"
                     /protein_id="HOI61280.1"
                     /translation="MARFPEAEARLLNVKVCMRCNARNAIRATRCRKCGSEELRSKSK
                     ERKA"
     gene            complement(12495..13145)
                     /locus_tag="PLI76_04275"
     CDS             complement(12495..13145)
                     /locus_tag="PLI76_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1917 domain-containing protein"
                     /protein_id="HOI61281.1"
                     /translation="MEEIDPESLGEVAYGIFEVYLNRELRHLGSHLFELVEQGTDFTD
                     DAARVFERFGEDYPDLAGALIRRFGGIDVVYASIRAGEGILPSKTTRMYWIVQDAPGP
                     LERGLDDEQVGKWLIFVPPGEADEAWRKIRDATARGELGISAKVSTAKPNPDSRDERA
                     VMYVYTRDWADEADVMRVRKRLRDLGFTERIGYKRNIETYRGEYSEEGRKVTYYSA"
     gene            13410..14291
                     /gene="htpX"
                     /locus_tag="PLI76_04280"
     CDS             13410..14291
                     /gene="htpX"
                     /locus_tag="PLI76_04280"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HOI61282.1"
                     /translation="MKWTRDFGLTMRMLLTSFLLLIVYLIFLGVLYALGFPFEFLLVV
                     AAGMAFLQYFFSDKLVLWSTGTRIVAEDEYPELHRMIEGLAVRAGLPKPQVGVMPSPV
                     PNAFATGRSPSHAVVAVTDSIMRMLTREELEAVLAHEMAHVKNRDMLTLTMASFLSML
                     AFLIMRNWFFMSLFGGGNRDNNMGALILVYVVSIVVWLVSTLLTRALSRYREFAADRG
                     SAELTDNPRALISALKKISGRMDYIPAEKKQEVEGANAFFIIPALSGNTLMELFSTHP
                     SLEKRVAALEDLAAGAP"
     gene            14709..16076
                     /locus_tag="PLI76_04285"
     CDS             14709..16076
                     /locus_tag="PLI76_04285"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOI61283.1"
                     /translation="MALKRVFGIFAVICILALAGPVVAVQVPEDQSQFVFPGEIREAG
                     AAVAFAKGDYLADRFPENMEVIGRYNVTIDPAEVFKLQPFNLDNPIEVKRDTPIGALD
                     AVARTGALNYSTYLYSTRNLLVIDHINGHLYQGDWLWLVVYENGSYITSTPDMWDYSI
                     SNGETIWLIYCDLTDFNSRYESRLDRAVAGLSITTTHGKSNVTPGPVTPEPVTPEPVT
                     PGPVTPEPVTPDPVTPEPVIPGPATPEPVTPGPATPEPVTPGPVTPEPVEGMNLIDVI
                     AENDNLSFFAVLLNATDLVTTLQSGGPYTVFAPSNDAFGNMPREAFASLLTDDGERMR
                     VLSAHVVNGSYTTADLLNVTRGGNTTTVETLAGGNLTISANGSVLSIDNATIVAPDIN
                     ASNGVVHVIDRVLSPPAEGPGPTVPAGVVLPPTTPATVTPVPAIPALTTPPLVTPMLT
                     MPAVG"
     gene            16474..>16792
                     /locus_tag="PLI76_04290"
     CDS             16474..>16792
                     /locus_tag="PLI76_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61284.1"
                     /translation="MSGVWRYAVVIVCILALFPAGAASGSVQALDLGTNVWVDGAPLT
                     PPIHEDREDPFPYAVNASSEGVWVAYNHTPGDRKAFICRDSVTGEDYTINATCAAIGE
                     HCYV"
BASE COUNT         3112 a         5189 c         5354 g         3137 t
ORIGIN      
        1 agtacttcga gtactcggcc tgcgggagac cgatcctctc cacccccatc cccgacatgc
       61 tccggatggg cggcggcagc atcgctgtct accgcgataa ggaggagttc atcgagaagg
      121 tcgacgagct tgccggaaat cccgggcgct gcccggcgga ggtcgaggga cacagctgga
      181 agaagaaagc agaggcattc gagaaggtct ttgcacggtt gataggaaaa gtctggtaaa
      241 cgcttctttt ttctgctctc ccggatacgc caagattatt aatggttcaa agggaaacag
      301 aaacgtagca aggagggtcc gatggctcac gtggatctcg ataaatatcg accgtatatc
      361 atcattggtg ttatcgttct gctcaccctg ctccctctct gggtacgggg tttcattccg
      421 gcagccgaca tggtgacccc cgacggggtc aacctcctcg gcaacgaccc ctggtacaac
      481 gtccgtcagg tcgaacagac cgtcgcgaac ttccccggtt acgcctggtt cgacgccatg
      541 acgctctacc cgagcggcga cgtcgtctac tgggggccgc tcttcatcca gatcatatcg
      601 gcgctctgcg tgctcgtcgg ggcaacgacg cgccccgaga tcatggtggt ggcatcctgg
      661 gtcccgccgc tgatgggcgc ggcgatggtg ccggtcacct acctgctcgc gaagagactc
      721 accaactgga agaccggtct catcgcggcc ggcctcatcg cggtcgtctc cgggagcttt
      781 gcccaccgct ccctcttcgc cttcgtcgac caccacatcg cagagaccct cttctcgacg
      841 atcttcgtcc tcgcctacgt cgtggctctg cttgcctccc gcgacatatc gctctcccgg
      901 aggagctctg aggtcctgaa gatgccggcg ataacggcgg cgttcgccgg tgtcgcctac
      961 ctcatcggtt tcttcaacat gccgacgatg atcctcctcg cgcttatcgt cgcggtcttc
     1021 accctggtcc agttcgtcct ggactttgtt caggaccggc cgagcgatta cctggtcctc
     1081 gtgaacgccg tgacgttcgg cgtggtgatc atcgggacgg cggcgtttgg cttcccgcac
     1141 ccgaacctcg acctctcccg ctacaccgcc gggcacgtcg tcgcctgtgc cggcctcatc
     1201 ggcgggacgc tcgtgctcta cgggctctcg gtctacctcc ggggacgccc gaagtactac
     1261 ttcccggcag cccttgcggg cctcggggtc gtcgccgtcg cagtgctctc catcgccctg
     1321 ccggatatct acaacctcct catatcgagc ctcttcgcct tcttcgggga agcgcccgtc
     1381 accacgaccg tccaggaagc gcgggcctgg tcgtttgacg cggcctgggc gacgttccac
     1441 tggggtctcg tgctcgcggc cggcggcgcc gccaccctga tctggtggag ccgcaaaaag
     1501 gtcaacccgg cccacgtctt catcctcgtc tggacggcga tcatcctcgc atcgacggct
     1561 gcgcacgtcc gttacgagta ctacctcgcg gcaaacatcg ctcttctcgc cgcgatcttt
     1621 gccggggcga tcctcgacgt aacctacaaa gacgtcacgc gcctctttgg ggcgaagagc
     1681 agcaccgccc cgcccgatgc cgaagttgcc aggaaagagg agaaacctgc acgaaagggg
     1741 aagaagggag cgagggcccc ggatgcccga aaaacgaaag tctcatcgaa agatcagccg
     1801 gactacatca aggtcggagc ctttgccgcg gtcgtcgtcg tcacgctcgt cttcgccgga
     1861 acctccctcc agaccaactt cgcctatgcc gcaagcgcgc agtacagcgg catcaactcg
     1921 cagtggatgg aggccctgga gtggatgggc acacacacac ccgatcccgg tgtcgactac
     1981 catgccatct acgaccagga cacgttcacc taccctgaag aatcctacgg cgtcatgtcc
     2041 tggtgggact acgggcactg gatcaccttc atagccaaac ggatcccgaa caacaacccc
     2101 ttccagcacg gggtcgccgg accgaacgga tcggcgacct acttcgtctc cacatccgaa
     2161 gaggaggcga accgggttct cgacaacgtc agcacccggt acgtcatcac cgatatcgat
     2221 ctcgataccg gaaagttcca cgcaccggcg acgtgggcca accccgatgt ggggagagac
     2281 cggtttgagc cgacgttctt catccccgtg agtgcgggtt cgacgagcta ccaggcgatg
     2341 acgttctaca accagcagta ctacctgacg atggtctcgc gcctccataa cttcgacggg
     2401 tccatgaccg acccgacgtc gcaggtgatc tacgccgagt accgcgatgc ggatgcggca
     2461 aacgcctccc tcccggtcat cacgcgcacc gagcagatga acgccaccgc tgcggcggcg
     2521 gcggtcgagg cttacaacaa gaacgcaccg gcgggttctc acgcgacgct cctgaacatg
     2581 tactaccagt acaggggaga cgcgatcctt cagccggtcg agcaggtgcc ggcgctccag
     2641 cactaccggc tcgtccatga gtcgccccag aacgtctttg gtaacgtcgg cgcaggcggg
     2701 cctgacctca agtccgtcaa gatattcgag tacgtcccgg gcgcccggat caagggcgac
     2761 gggatcatcg aggtgccggt cgtgacgaat gccggccgcg cgttcaccta ccggcaggag
     2821 agcgtgaacg gcacgttcat cgtcccgtac gcgacgtccg ggtggtcggg cgaggtgaag
     2881 acgaccggac cgtaccggat cgcgggcacc gggcagacgt ttgacgtcac cgaagaggat
     2941 atccgacagg gacgcaccat caactaaaca gggatgcgag cttcggtcat atacggtgac
     3001 atctttaccc ggcacgacat ggaggcgcac cccgagtcgg gctcccggtt gctgggggcc
     3061 ctcgccgggg tacccgagaa tgtgcggtgg cacgccccgg tccgcgctac cgagcaggat
     3121 ctggagcggg ttcaccgccc ccagtatatc cggtgggtgc aggagatcgc gcgaggaccc
     3181 tgttttttag acccgaacac ctacgtcacc tgccactcct tcgagacagc gttgtacgcc
     3241 gccgggtcga cgtttgcagc cgtggagcgg gcacttgacg gcgagcacca gtttgccctg
     3301 gttcgcccgc cgggccacca cgccgagccg gaccaggcga tggggttctg catcttcaac
     3361 aacgccgccg tcgctgcggc aaaagcgctc ctgtcggtcg accgggtcgc catcgtggac
     3421 tgggatctgc accacggcaa cggcacgcag acggcttttt acgggagcga ccgggtactc
     3481 ttctgttcgg tgcacgaggc cgatagtttc ccgaagaccg ggtgggtgga cgagatcggc
     3541 accggcgccg gccggggcta caccctgaac gccccgctcg cggcagggtc caccatcgcc
     3601 gactaccagc atgtctttcg cgagatcttc gtcccggcgc tcgtccggtt ccggcccgac
     3661 gtcctgatcg tatcggccgg gcaggacgcc ctctccgacg acgaacacgg gcggatgaac
     3721 ctcgaacctg aggattacac cgcgctcacc gggatgttga tcgacggcac ggatctccct
     3781 cttgccctga ccctcgaggg cggatacggt ccgtcgcacg ggaaggcgat cgccgcgata
     3841 ttccgcgcac tctcgggaaa acggcatact cctgcagaga gacccctgca ccggaccacg
     3901 gagagggtcg tcgatatctt aaaaaaggtc cggttctgct gagggtaacc cgcgcggccg
     3961 acacgcgcaa cagcggtgat accatcttac acgttgcagc gaaggtgggg actcccaccc
     4021 ggcgctctcg ccgggacgag ggcccaaggc ttgcaggtta tcacaatcgc gctcctgttc
     4081 gtcacgattg caacgatgcc ccattgccgg cactatcgat ggagggagta tcgttcttct
     4141 ccccggtgcc ggggagtttg cagccttcgg ggcatggttt ttcgccggct atcgccatga
     4201 ccgccccatt ccccggaggg agcggggttg ccggagaggt atatcgatgt cctttcacag
     4261 gccggtccgg gagggggatg ggtatgcatg agcggcctcc ctgtgggaga gcccgcgccc
     4321 ctctcccggt tcattccccc ccgggggaac ctctcacccc tgcgcggggc gtttccggtc
     4381 ggggtccatg taggagacct caaacccggg gacgacgacc cgcaccaccg gcaccggggt
     4441 tcttgagaga tcgcagacgc agacccggtc cgcatagggg ctgatctctt gaagcgcccg
     4501 ctcgatatcg aggtcgaacc ggttggtgct cacgtcgggg atatccctga tatcgacggc
     4561 ctctgcatcg gcaaaccaca tccggttgat ccgcttcagc cgctcgtaac ccgccttcct
     4621 gatgaccatc tcccgctgag ggtcggtgcg gccgccctgg agataactcc cgcggctctg
     4681 ggcgacctcg gtcagggcgc ggagcgccgc gatctccggg caggcatgcg tccccgaccc
     4741 gatgacgatc atcgccggat ccctcgtgac ggtatcgtcc gcagcggccg cgacggtggg
     4801 gatgccggtc ttcccgtcga gcaaccagag gtggatctcg ataccgttct cctcgaaccg
     4861 gtcgagaact tcccgtgcgg cgcaatcgtt cccgatggtg agacggtggc cgaggtcatg
     4921 cctctgctcg gcgaggctga gcgcatcccg ttcgatcacc tcaaagaggg cgtgcaggat
     4981 cgcctcttcc atgacgtttc ccgatgcaag cccgtttgaa tcgctccgga agagcggcac
     5041 ggccataccg agcgagtcgt agggatggaa gacggcgttg ctcgggacat aggcctcctc
     5101 gtcgttcagt atatcccata ccggcgtcca gtggagcttc tccccctgtt cgagtttgcg
     5161 cgggacgaat agatcctcgg gatgtacggc ccgccccggg ccgatctcct cgtaggttgc
     5221 atactccatc cggtcacccc ggtactcggc acagtaccgc tcgagtgcct ccatcatcgc
     5281 cgagaccctg gcgtggatgg gctccttccc cttccccgcg tggacgcggg ctgccccgcg
     5341 ggctgctccc ggccggaccg ccgtaaagac cgggatcccg aggcggtcga gaggggtcac
     5401 gtcgacgatc tccgtcacgc cgatctccgc catcaacggt tctacagcgg cataggtctc
     5461 ctcgggagac ctggagcggt gcgttccgtc aaagtagagt ttctctaccg ggtgaatcat
     5521 gattgccatt taaagtacct tggaggtgat accttaatag tatggcaggt ataccctacc
     5581 cacgtatgag cacggatgat gtggtggtgg gcgcaatcgt tcggtacccc cgcaccggca
     5641 cgacgggaaa ggttctccgg gtcgaggata tcgacggccg gcggtatgcc gaactcgaca
     5701 gcaccggact ctactaccgg gttgacgagc tcgtcggcgc cgaccgcacg accggacccg
     5761 tggagaggga ggggcactcc ctggaggatt acctcgcgga gcacatggag ttcaaagagc
     5821 aactggaaca ggtgtgggag aaagggatcg accagagctg tgaaggcggc gggtaaaccg
     5881 cccttcatca ccgctttttg atcagggcgt gaaatctccg gcatcaaccg gggggcagtt
     5941 ggtccgcggg ggggttaccc ctcgatcggt atggtggtga gtttcaccgc ctcgacgccc
     6001 ttctgcgcca tcagtcgttc cgtaatatct ttcagcaccg ctccatcacc gcggatcagg
     6061 atgacctcaa ggcacctgtt gtgggtcacg tgcgagtgaa gggatgcctg gatgatatgg
     6121 gcatactggt gctggatctc ggtgagcgtc tgaagcagcc cgcgctggtc atggtcgtag
     6181 accatggtga tgacgccctg acgctcccct ttcacatcgg agatccactg gtaatgagtg
     6241 atgtaactcc gtattgagtc ccgtatccct tccgaacggg aggaataccc ccggaggctc
     6301 aggatctcgt cgaatttatc aagaaggttc ttgggtagag agatcccgat acgtgaaagc
     6361 tcggtatcac ctgacatact gtaaccagtt ttcatatctc tcctggtaag gtataaattt
     6421 ttgctcacat tccggcgcat tattcatact tcgtatgccc cgaaaaaggc agcaaaatag
     6481 ggggccgccc ggatgtgcga taagaatgca accggggccg gagagatgat cctcaccgat
     6541 tatgtataag tagtgtcact ctatattgga taaggaggtt cgaaaacttt tgcgagatgc
     6601 attcattccc ggtgtcaata ttgggctcgt gggtcatgtc gatcacggca agaccaccct
     6661 ggtcagcgcg ctgaccggta cctggactga ccggcacagc gaggagatca agcgcggcat
     6721 ctccatccgg ctcggttacg ctgatacgac tttttaccgg tgtgagagat gtgagggggc
     6781 ggaggcctac acctcccggc ccgagtgccc gagttgcggg ggaaaagcgg tcccgttccg
     6841 gacggtctcg ttcgtcgatg ctcccggcca cgagacgctg atggcgacga tgctctccgg
     6901 ttccgcgctg atggatggcg cgatgctcgt catcgctgca aacgaggtct gcccgcagcc
     6961 ccagaccaag gagcacctga tggcgctcga gctgatcggc ataaaaaaga tcgtcatcgt
     7021 ccagaacaag atcgatgtgg tcacccaggc cgaggcgctc gagcactaca agcagatcaa
     7081 acggttcatc aaaggcacga tcgccgagag cgcgcccatc atccccgtct ccgcgcagaa
     7141 aggggtcaat atcggcgccc tgatccagac ccttgattcg atcgtcccgg aaccagcccg
     7201 gaacccggat atcgatccgg tgctgttcat cgcacggtcg tttgatatca acaaaccggg
     7261 ctgcagctgg cgggacgtga agggcggcgt catcggggga tcgctcatcc gcggcatcct
     7321 gcacgagggt gacgatatcg agatccggcc cggccggcag gtccaggtgg agaaccggat
     7381 gaagtgggag cccgtcgaga caaagatcac ctccatcaac gccggcaaga cccgggtgac
     7441 cgaggccgcg cccgggggcc tccttggcgt tgcgacgaaa ctcgatccgg cgctgacgaa
     7501 gagcgacgcc ctcgccgggc aggtggccgg gctcgtgggg aagctgccgc cggtatggga
     7561 gcggctgaag ttcgatgtca cgctcatgga ccgggtggtc ggtgcggata gcgagcagat
     7621 catcgagccc ctgaagcata aagaaccgtt gatgctctcg gtcggcaccg cggtcaccgt
     7681 cggggtgatc gtgaacacga agaagaacca ggtggaggtc cagctgaagc gggcggtctg
     7741 tgcggaggtc ggcgcacgga ttgccatcag caggcaggtc ggcgggcgat ggcggttgat
     7801 cggcatgggc gttctggtcg agtgagggtg ctcctcgata cgaacgccct cctgatgccg
     7861 gcccagttcg gggtcgacct gtatgacgag cttctggccc tctttgggga tttcgagccg
     7921 atcgcgctcg aagaggtggt cggcgagctc tcggggcttg cgcggggccg gggccgcgat
     7981 gcggcagccg cccgcgtggg ccttgcgctg gtccggcgtt cgacggtcgt gccgagcggg
     8041 agtaccgccg agggcgtgga taaccgggtg atcgagtatg cccggcggga agggtgtacg
     8101 gtagtgacga atgaccggga actccggaac gctctccttc gcgagggagt cgacgtcgtc
     8161 tcgatgcgaa ggcagaagac gctggagttg ttgaggggat aaactatgta ttacaagatg
     8221 acgctggagg ataaggtgcg ggttccgccc caccgcctcg gggaagacct tggtgcggtc
     8281 atcctcaatg tactgcagga acagctggaa ggcagcatcg caaaggagat cggtatcttc
     8341 attgcggtca cgaagatcat cgatatcggc gaaggggagc tgatccccgg ggacggtgcc
     8401 gtctactacg acgtgcggtt cgaggcggcg gtgctccgcc tggggctcca ggaggtgatc
     8461 gagggcgagg tggtcgagac gacaagtttc ggcgcgttcg tcagcctcgg gccgatcgat
     8521 gccatgctcc acgtgagcca gatatccgat gaatatatca actacgacga gaagaacggc
     8581 aggctgatct gccaggactc aaagcgctac atcgccgtcg gtgatgcgat ccgggcccgg
     8641 gtcgttgctc tctcgttgaa cgaacgcgag ccacgcgaga gcaagatcgg cctcaccatg
     8701 cgtcagccgg ggctcgggac ctcggtctgg ctggaagagg atctcgaaga ggagaagcag
     8761 aaaggagcag cgtgatatgg tcgtccgtaa gaaggtggtc aaggtctgcc gcgagtgcca
     8821 tcgggtcgtc gaaggagagg cctgtgtcat ctgcggcacg gccaacctga gcgaagactg
     8881 ggcgggctac gtcgtcatca tcgatcccga gcattcggat atcgcaaaga agatgaagat
     8941 caccctgccc ggccggtacg cactgaaggt ccgttgatgc tcaggctccc cgaagcgcac
     9001 cgggatctct tcaaaaagcc gttcggcacc ctttatggaa gtatcggcga actcctcccc
     9061 cggctttcgg gccgggcggt ctacgccgtc ggcgatgtgg tgacccataa cctcctcgac
     9121 gccggggtca tccctgacgt cgccatcatc gacggctata cgatgcgcct gccctgcacc
     9181 cgcctgcccc tgttccgggc gagacggcgg ttgacggcga agaaccccgc cgggacgatc
     9241 accgacgagc tcgcgggcgc gatcgccgat ctggtccggg gtccgccggg agtgctcttc
     9301 gtcgacggcg aggaggatct tgcggtcatc ccgctcgtcc ttgcagcgcc ggacggtgcc
     9361 gccatcctct acggtcaacc gggagagggc gtcgtcctcc ggatcgtcga tgaaccggcg
     9421 aaacgggagg ccaggttcat gctgacctgt ttcgtgcgcg agtagggtgc ggccagcccc
     9481 ataccataat ttaaataaat ctgtcaacaa cattttaggg atatcgatgg atttcgaaat
     9541 tacccgtgac gtgaggaacg aggtgttgag ccggagggaa cttacgttta ccctcacctt
     9601 tgacggcccg accccctcgc ggaagagcat ccaggagaag ctcgccgcaa tgctgaataa
     9661 gaacgagaac cttgtcgtgc tggaccttga gcggacccga ttcggggcga tggaactctc
     9721 cggccgcgcc cggatctacg acgacgagga gaggaagaag tcgaccgagc ggggatatct
     9781 gctcaagcgc ggcgagccga aggccgagag cgaggcgtga agggcatggc ggtcaagaga
     9841 cacgagtgct acgaactcaa gggagataag gtcgttctgc agaagcgcca ctgtccccgg
     9901 tgcggccccg gcgtcctgat ggcggagcac aaggatcggg tggcctgcgg caggtgcggc
     9961 tacaccgagt tccggaagta gttcctcact ctccatacct atacggtcac ggcgggagat
    10021 tccggccgcg gccatctttt tcgatggggc gatacctgtt ttatgtctga tacgagatcc
    10081 gaagacgggc tggtgctggg acttgagggg acggcgtgga acctcagtgc cgccctcttt
    10141 ggagaagggc tggtcgccct ccactcctcc ccctatatcc cgcaaaaggg cgggatccat
    10201 ccgcgggagg ccgcgcagca ccacgcatcg gtgatgaagg aggttgtctc ccgggtgctc
    10261 acggagccgg agcgtatccg ggcgatcgcc ttctcccagg gacccggtct cggcccttcc
    10321 ctccggacgg tggcgaccgc cgcgcgggcc ctcgcgatcg cgcttggtgt gccgctcgtc
    10381 ggcgtcaacc actgtgttgc tcacatcgag atcgggagat gggcgaccgg gtttgccgac
    10441 ccgatcgtcc tgtatgcgag cggcgcgaac acgcaggtgc tcggcttctt gaacggccgt
    10501 taccggatat tcggcgagac actggatatc gggcttggga acgcgctcga caagttcgcc
    10561 cggagccacg acctcccgca cccgggcggt ccggccatcg agcggcttgc gcgggaggga
    10621 aactacatcg aactcccgta tacggtgaag gggatggatc tcgccttctc cggtctcgtc
    10681 agcgccgccc aggagagcac ggccccactc gaggatgtct gcgcgggcct gcaggagacc
    10741 gcgtttgcca tgtgcgtcga ggtgaccgag cgggcgctcg cgcatgccgg caaggacgag
    10801 gtgctgctcg tcggcggggt cggggcgaac gcgcggctgc aggagatgct ccggacgatg
    10861 tgcgaggacc ggggggcgtc gtttgccgtc ccggagcgga cctatctcgg cgacaacggg
    10921 gcgatgatcg cctatacggg gaagatcatg ctggagcacg gggttacgct ccctatcgag
    10981 gagtcgcaga tccgccccgg gtaccgggcg gacgaggtgg aggtcgcctg gtggatcggg
    11041 cccggcgaga tctttgctgc cggacctctg cccgagggcg gtatcgcccg gggcgcggag
    11101 gcggtcgtgg agatccggga aggtgatgtg gtcaagcgcc ggacgggcaa gcgctaccgg
    11161 aatccgggcc tcgaccggcg gttgattgcg gagcggaccc gggccgaggc ccgcctgatc
    11221 gcggcggcgc ggcgcgcggg ggttccgacc ccggtgatcc gggacgtcac ggccgatacg
    11281 atcgtgatgg aacgggtcga gggcgacgtg ctgaagtacg tcacgtccgc cgaaaacgtc
    11341 cgcctcgccg gcaaagcggt cgggaggctg cacggggcgg gtatcgtcca cggcgacctg
    11401 acgacgagca acatgatcgt ccgcggcgat cagtgcgtcc tgatcgactt cgggctcgcg
    11461 tcgacgtcgc cggaggtcga gagccgcgga gtcgacctcc acgttttatt ccagacgctc
    11521 gagagcacca cggagaacta cggggagttg cgggaggcct tcgtcgcggg gtatgcggag
    11581 acgttccccg gggcggatga ggtcctctcc cgcgagcacg aggtggaact gcgggggcgt
    11641 tacctgtgag gctcgcggtg gtgacgagca acgcgaacaa ggcgcgggag gcggccgcgt
    11701 acttcgcggg ggtgctggag gtcgagcacg tcgccctgga atgcccggag ttccgccacg
    11761 tcgatatcgg ggagatcgcg cgcggcaagg cggagttcgc ctacggggtg ctctcccggc
    11821 cgctgatcgt cgacgatacc ggcctcttca tcgacgccct cgacgggttc ccgggcccgt
    11881 gcgccgcgta cgtccacgac acgatcggga acgcaggagt cctgaaactc atggagggcg
    11941 tggagaaccg cggcgcccgc ttcgagacgg cgatagcatt cgcccgcgaa gacggtatcc
    12001 ggatctttcg cgggatcctg cccgggacga tcgtcgcccc ccgggggatc ggagggtttg
    12061 gctacgatcc gatctttgcc tctggagagc agacgctcgc cgagatgccg cttgcggaga
    12121 agaaccggat atcgcaccgg gcacgagcac ttgaggcgtt tcgcacatgg atcacgcagg
    12181 agggtagctc ctgacaaact gttaatagga ttgagaacga acagtaccca atcgacaaat
    12241 ggtgttttcg acatggcacg atttcccgaa gctgaagcac gtctgctcaa tgtaaaggtc
    12301 tgtatgagat gcaacgcccg gaatgcaatc cgcgcgaccc gctgccgcaa gtgcggctca
    12361 gaagagctcc ggtccaagtc caaggagcgc aaggcgtaac gccgtcgtat ctgcggactt
    12421 ccggccacgg ccgggccggg gatccggtat actcttttac acgttcgccg aggagagcga
    12481 aggttatctg cccttcaggc actgtaatag gtgaccttcc ggccttcttc gctgtactcg
    12541 ccgcgatagg tctctatgtt ccgtttgtaa ccgatccgct ccgtaaaacc gagatcgcgg
    12601 agccgctttc tgactcgcat cacgtccgcc tcgtcggccc agtcccgggt gtagacgtac
    12661 atgacggccc gctcgtcgcg cgagtccggg ttcggtttgg cggtgctgac ctttgcggat
    12721 ataccgagtt ctcctctcgc cgtcgcgtca cggatcttcc gccacgcctc gtcggcctcg
    12781 cccggaggga cgaagatgag ccatttcccc acctgttcgt cgtcgaggcc ccgctcaaga
    12841 gggcccggcg catcctggac gatccagtac atccgggtcg tctttgaggg caggatgccc
    12901 tcgcccgccc tgatcgatgc atagaccaca tcgatcccgc cgaaccggcg gataagcgca
    12961 ccggcgagat ccggataatc ctccccgaac cgctcgaaga cccgggcggc atcgtctgta
    13021 aaatcggtac cctgttcgac gagctcaaag aggtgcgagc ccaggtgacg gagttcacgg
    13081 ttgaggtaga cctcaaagat cccgtacgcg acctcgccca gggactccgg atcgatctct
    13141 tccatactta ctggtaactg ccgggaccgt aaaaagggtt ctgtagtgtt atcggcacgg
    13201 ccgggccacg cgagactctt gctcttcttc cgggcggcca cggcatcgtg ccgggtgcgc
    13261 cgatggtgcg ggccacggtg agcgggagcc cggcatgccg ctacgttctc ctgagggcgc
    13321 cggatgcgcg aaaagccggt ccggacgtga gcgcccgggg cgattccggc gtatatctta
    13381 atagcgatcc ggtccaatag atctctgtca tgaagtggac gcgcgacttc ggtctgacga
    13441 tgaggatgct tctcacgtcg tttctcctcc tcatagtcta cctgatcttc ctgggcgtcc
    13501 tctacgccct cggatttccc tttgagttcc tcctggtggt tgctgccggg atggcgttcc
    13561 tccagtattt cttctccgat aaactcgtgc tctggagcac cggcacccgg atcgtcgcag
    13621 aggacgagta cccggagctg caccggatga tcgaaggcct tgccgtgagg gccggcctcc
    13681 cgaaaccgca ggtgggggtc atgccttccc cggtgccgaa cgcgtttgcg accgggagaa
    13741 gcccgagcca tgccgtcgtg gcggtgaccg actcgatcat gcggatgctc acccgcgagg
    13801 aactcgaggc ggtgctcgcc cacgagatgg cgcacgtgaa gaaccgggat atgctgacgc
    13861 tgacgatggc gagtttcctc tcgatgcttg ccttcctgat catgaggaac tggttcttca
    13921 tgagcctctt tggcggcggc aaccgcgaca acaacatggg tgcactgatc ctggtctacg
    13981 tcgtctcgat cgtcgtctgg ctggtgagca cgctcctcac ccgtgcactc tcccgctacc
    14041 gggagtttgc cgcggaccgg ggcagcgccg aactgacgga taacccccgt gcgctgatat
    14101 cggcactcaa gaagatcagc ggacggatgg attacatccc ggccgagaag aagcaggaag
    14161 tggagggtgc aaacgccttc ttcatcatac cggccctctc ggggaacacc ctgatggagc
    14221 tcttctcgac gcacccgtcg cttgagaagc gggtggccgc ccttgaggat ctggcggccg
    14281 gggcgccgta acggccttcg gggctatata agactcacct ttttttgcga ctccgttgcg
    14341 gcgatgccgg aggggttcgt gcctgtatcg gcgccgcggg tcgctgtagc ggggatcttc
    14401 tcgcggccag atcccccggg cagaaactgc tgccgggaca gtatcgtacc ccagaacccg
    14461 gggtgattgg gcagctgatc gtcacatcag ctggcttgca attttatctt accaaaatta
    14521 tataacattc ttttgccaag agatgcccgc tctgtcagtc acggccgaat acccccggga
    14581 ggtctggcgg ctcagcccgc agcaccgcgc aggtgcagtc cggggggcgg atccagttcc
    14641 cgctgtcgtc ctcatcacgg cccggctcca gtcacgagcg cccgggtcag gcagaaaagg
    14701 tgattacaat ggctttaaaa cgagtcttcg gcatctttgc cgtcatatgc atcctcgccc
    14761 tggccggtcc cgttgtcgcg gtccaggtgc cggaggatca gtcacaattc gtcttcccgg
    14821 gcgagatccg ggaagcgggc gcggcggtag cgttcgcaaa gggggattac ctcgcagaca
    14881 gatttccaga gaacatggag gttataggga ggtataacgt cactatcgat ccggcggagg
    14941 tcttcaagct ccagccgttc aacctcgaca accccataga ggtgaagaga gatacgccga
    15001 tcggcgcgct ggatgcggtg gcccggacag gggcgctgaa ttactcgaca tatctctatt
    15061 caaccagaaa tctcctggta atcgatcaca tcaacggcca tctctaccag ggggattggc
    15121 tctggctcgt agtctacgag aacgggagtt acatcacatc aacccccgat atgtgggatt
    15181 actccatatc caacggtgag accatctggc tcatctactg cgatctcacg gatttcaact
    15241 cccgctacga atcacgcctg gaccgggcgg ttgcaggact gtcgattaca accacgcacg
    15301 gcaaatccaa cgtaactccc ggccccgtta ctcccgaacc ggttacccct gaaccggtta
    15361 ctcccggccc tgttaccccc gaaccggtta ctcccgaccc tgttaccccc gaaccggtta
    15421 tccccggtcc tgctaccccc gaaccggtta ctcccggtcc tgctaccccc gaaccggtta
    15481 ctcccggccc tgttactccc gaaccggttg aggggatgaa tctcatcgat gtgatcgccg
    15541 aaaacgataa cctgtcgttc tttgcggtcc tgctcaacgc aacggatctg gtgaccacgc
    15601 tgcagagcgg cgggccgtat acggtctttg cgccgagcaa cgatgctttt ggcaacatgc
    15661 cgcgcgaggc cttcgccagt ctcctcaccg atgatggcga gcgaatgcgg gtgctctccg
    15721 cccacgtggt caacggcagt tatacaactg ccgatctcct gaatgtgacg cgaggtggga
    15781 acacgacgac ggtcgagacc cttgccgggg gaaacctgac tatctctgca aacggcagcg
    15841 tgctcagcat cgataacgcc acgatcgtcg ctccggatat caatgccagc aacggcgtcg
    15901 tccacgtcat cgatagggtt ctcagcccgc cggcagaggg tccggggccg accgtgcccg
    15961 cgggggtggt gctgccaccg acgacacccg caacggtgac tcccgtgccg gccataccag
    16021 cgctgaccac acccccgctg gtgacaccga tgctgaccat gcctgcggtg gggtagtggt
    16081 gaccacggca tcgccggccg ccgtggagga tgtccgcccg ggaacggcac ccgactgccg
    16141 tttccacctc tccgtaatta gtggaaaccg ggatgcgctt cgtgagcggg aggctttgcc
    16201 ggcaggatgt agcaggtttt ttagcctgga ggggcgccgg agtcgggcac cttcccgggg
    16261 tttttcggga gcgttcggca atcctgtatc gactatcccc gtcgtgcggt tgtgcgatgg
    16321 gggtagcgtc ccgggccggg cggggggagg cgatcccgga tcctgcaacg gtacgcagga
    16381 gagaggcccg gccgagaaac ccgcgggggc tatcccgtgc tgtatctttc ttcgcggcat
    16441 gacaggccgg atggccgaga gcgggggtgc ggggtgagcg gcgtctggag atatgcggta
    16501 gttatagtct gtatcctggc gctcttcccg gccggagccg catccggaag cgttcaggcg
    16561 cttgatttag gcactaacgt atgggttgac ggagcgcccc tcacccctcc tatccacgag
    16621 gaccgggaag acccgttccc gtatgcggtg aacgcgagca gcgagggcgt gtgggtggcg
    16681 tataaccaca cccccggcga ccggaaggcg ttcatctgca gggatagtgt aactggtgag
    16741 gattacacca tcaacgcaac gtgtgcggcg atcggggagc attgttacgt gt
//
LOCUS       DAOSEE010000036        16412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1430_length_16412_cov_237.617534, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000036 DAOSEE010000000
VERSION     DAOSEE010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 16412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16412
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1430_length_16412_cov_237.617534"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(405..1046)
                     /locus_tag="PLI76_04295"
     CDS             complement(405..1046)
                     /locus_tag="PLI76_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61285.1"
                     /translation="MDSAITFQCAFEGSLDGAVVRRLLRHAGALPGDLYRQRKSYLLE
                     KLQGFNVSARARPWVVVVDLDHDAGCAPEVVRNWLPAPSYFMNFRVAVREVEAWILAD
                     RERLARYLQVPETRITGTPEEIDYPKEYLINCARESSSSRIRKGIVPGPGSRRVEGPA
                     YLSLLTEFVADAERGWRPDVASDHSESLERCIRSLQNSIGTFSRESQRRRSSR"
     gene            complement(1048..2190)
                     /locus_tag="PLI76_04300"
     CDS             complement(1048..2190)
                     /locus_tag="PLI76_04300"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOI61286.1"
                     /translation="MPKRFTHISLWNWRNFQRVDMDLQARVFLVGPNASGKSNFLDVF
                     KFLRDIVSVGGGFQGAVKERGGVSSLRALSARKYSDIGIMVRLGNAERDDLWEYELTF
                     RQDERNLPVIAKERVARCGNEVIARPSAQDEEDPERLTQTYLEQVNVNKDFREVADFL
                     KTTRYLHLVPQLVREPDRSVGYRNDPYGSDFLKQVMGRSKEERDSMLRRIERALSVAV
                     PQLSEIKVEFDSIGKPHLYGRYKHWKPQEAWQTEQQLSDGTLRLLGILWALLDDGGPL
                     LLEEPELSLHPAIIRYVPQIFSRVQLKSDRQILISTHSSELLQDEGIGLDEVLLLRPS
                     EEGTTVVSAGNIEEIDDLLKGGLSLADVVIPKTCPGDTAELAWFGE"
     gene            2406..2711
                     /locus_tag="PLI76_04305"
     CDS             2406..2711
                     /locus_tag="PLI76_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61287.1"
                     /translation="MRILKITVEEHPDAYVACPPGLKGVVIAEGDTHEEALAHSNPDS
                     TTMPNHPQIKGLTLRSICTQAGIPREEFLRAYEQEQAIQFPAFSPARAPYYPAPQTC"
     gene            2714..3067
                     /locus_tag="PLI76_04310"
     CDS             2714..3067
                     /locus_tag="PLI76_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase family protein"
                     /protein_id="HOI61288.1"
                     /translation="MITDRERAAFEAGIKLGALYHQFVGTPIARETAAGVEAAIEQAV
                     GLQPYVTEIRVRLNCDMMVSNRFGYSELSGRMFDAEITTTVGATVCRARLHLEDDYPM
                     MEIVDIHETSRDTDN"
     gene            3039..3869
                     /locus_tag="PLI76_04315"
     CDS             3039..3869
                     /locus_tag="PLI76_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOI61289.1"
                     /translation="MKPPAIPITDDHIHIDPVNGRGVEAAKDFRRSGGTHIFLVSKPS
                     WSFGVEPSSGEDYREVFLATLRVADMVRETGLVVYPVLGVHPAEINRLAARMSLEEAA
                     GVMMAGLDLAARYVEGGEAVALKSGRPHYEVPPEVLAASNAILYRALELGADYDCAVQ
                     LHAESGPCTDVVDMAGRAGIPVERVVKHFATPDTPLMPSLIARHEDIPALARAGRHFT
                     MESDYMDENARPGAVIGPKSVPRFTHRYLKEGLITEEDAWRIHAATPSRTYGVDITLP
                     "
     gene            3914..4207
                     /locus_tag="PLI76_04320"
     CDS             3914..4207
                     /locus_tag="PLI76_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF424 domain-containing protein"
                     /protein_id="HOI61290.1"
                     /translation="MYLKVHHVPDAGEVVAACDSELLNVTLMHGDVEVCITGGFYGTE
                     RVAEEEVRRALLGATNANIIGKRVVALAVALGLVRREDCIMIGDVPHAQIFGM"
     gene            4211..5254
                     /locus_tag="PLI76_04325"
     CDS             4211..5254
                     /locus_tag="PLI76_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_function: GO:0043023 - ribosomal large subunit binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="60S ribosomal export protein NMD3"
                     /protein_id="HOI61291.1"
                     /translation="MAIQTSICPRCGQPTEEGLCPQCRAADTRLLACEPRVTAVYCPV
                     CDSQRRGKTWSDLRMPREDLIAEMAIAATSIHEDAKDIRVAVRSAETGPNRTTCTVDV
                     EGTLYSVPVHGTCEVEILWQKEACDRCSRISGGYYEGIVQVRATDRKINAYERSVAAS
                     IADQAEESLQQAGDRLSFVSELEDSRDGVDITVGSQHLGQEIARMITGALGGRYTTHP
                     KLVGEKDGKALYRVTYSIRLPFYQKGDVVVSHGGYFEVRDIDGRRLRAFDLATGATRT
                     LAEDEVERLIGNVREAESALVVFTQPDIVGLMDPKTYRTREQSPVPWTFPVEGQPIRV
                     LRDEEQDRLVIVG"
     gene            5264..6133
                     /locus_tag="PLI76_04330"
     CDS             5264..6133
                     /locus_tag="PLI76_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOI61292.1"
                     /translation="MRARRVPAAVLADIVGAEWVDPARRPYVRGGTAWVPVREGYPAD
                     ADLPERESYRGRGYHRIGDVAVLRGDAPSEAELAAIVEHCRPRGVLLVKGYLGDMRIP
                     DVEILYGNTGEVCHREQGYTFILDPARVMFAQGNRNEKARIASLVRPGERVADMFAGI
                     GYFTIPAAAGGAQVHAMEINPIAFEYLQRNSMANHVADRVTAENGDSRILLSGTYDRI
                     LMGHFDAPSMLADALAHAHSGSVLHVHSIGDVRGAIRDATAEAGFTAMVTSRRVKKYG
                     PHAWHMVQDVTIS"
     gene            6130..7260
                     /gene="coaBC"
                     /locus_tag="PLI76_04335"
     CDS             6130..7260
                     /gene="coaBC"
                     /locus_tag="PLI76_04335"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOI61293.1"
                     /translation="MKTFSGKTVVLAVTGSIAAVETVKLAHALRRRGAEVQAVMTEAA
                     TGIIHPDALTYATGRPAITRITGLVEHILYCGEGGAADLLLIAPCTANTIGKIAAGID
                     DTPATTFATTALGRGMPVVVAPAMHESMYRHPGVVENLERLRSWGIDIVAPRIEEGKA
                     KVADIDTIVLHAARAVSGRPLAGRRVLITSGACAEPFDDVRVLTTRSTGRMGRALALE
                     AFRLGADVTVVHAGSFPCVRNIRVATAADMRRAVLSVCRDDGVDIYASAAAISDFAPE
                     RCEGKIPSGSPLDIRLNPLPKIIDEVMATCRPVTVAFKLGWHEEERARTMLDAGVKMV
                     VVNAPPAMGAAGGTFSLMMADSTIEVTGSKEEVAAAIWSGLL"
     gene            7242..8084
                     /locus_tag="PLI76_04340"
     CDS             7242..8084
                     /locus_tag="PLI76_04340"
                     /EC_number="2.7.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate kinase"
                     /protein_id="HOI61294.1"
                     /translation="MVGTAVAFSPGHISGYFRRVEGSDPRTTGSVGAGVVIDEGVRAT
                     VVPAAKTGVRVIRQGRVSTGSPPVEYALDRLGVTARVTTECRLPIGAGFGLSAAALLA
                     TLTAVNHLFDLGLSADEVAARAHEAEVVHRTGLGDVAASRSGGVVCRMGPGIHADIAR
                     FYPDEPLYAVSLGPLPTASVLSSEEAMARIASAYPDRCPRDIADFCFLSRQFAERSGL
                     IAPDVRKVLSACDAAKIPASMTMLGNGVFAAGNSAERVLSGFGEVYRLNVARRGAYLI
                     EMRP"
     gene            8081..8812
                     /locus_tag="PLI76_04345"
     CDS             8081..8812
                     /locus_tag="PLI76_04345"
                     /EC_number="6.3.2.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphopantoate--beta-alanine ligase"
                     /protein_id="HOI61295.1"
                     /translation="MIPKDHPRYRSLIARERLARCAREGIVALEGLAAHGRGEAFDYL
                     IGERTSESAALAARTAAAMLLSARHPVISVNGNTAALAADEIAALQKASGADVEVNLF
                     HRTEERIRLITRLLEEHGVRVLSGTAERLVPLAHDRALSLREGIGDADVVLVPLEDGD
                     RCAALRAMGKTVITIDLNPLSRTARTATLTIVDELTRAVPAITVACASLSPGEGERLI
                     ASLDNIYLLRAAIDEMRERLSHALE"
     gene            8799..9809
                     /locus_tag="PLI76_04350"
     CDS             8799..9809
                     /locus_tag="PLI76_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOI61296.1"
                     /translation="MLWSEVYRPKRCEDIIGQDAVIRYLVSFADAGSVPHMILSGPHG
                     TGKTVAVECLAKRLYGENWEANTTVLSAADLLGRGRSALETDERFAQIYRKDQSLIVN
                     FKYVVKWYASMRPLDADFKLMVFEDAEGLTFEAQQALRRTMERYSATCRFIFVTTRPS
                     AIIPAIASRCLPLFFAPLESALIRTRLAEILAAEAATCPADDIDLIVHAARGDLRRAI
                     TYLQIVAGAGEEIDLAEVARSETSNVATSAFEALRAGNFAAARSIAESLMIEYGLPAR
                     EVVGELRRAVRREYNHPALAIALADADCQLGHNANDFVQINALLARIAREVSSEESTA
                     AL"
     gene            9784..10500
                     /locus_tag="PLI76_04355"
     CDS             9784..10500
                     /locus_tag="PLI76_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOI61297.1"
                     /translation="MKKVQQHYDEIADIYDDRYDGNRGKYYHGHISDHVMGVLPQGGF
                     LLDIGCGTGLFVQRYVEEGGRAVGLDISPGMVRHGRQRCPASGFFVGTADVLPFKDAT
                     FDALASLLAFSYVPDPEAMLRECYRILKPGGRIAVCTLSRTVFTSIVPIAYQVGEKVG
                     LKKVGVGDFGEHYYTNSEIARLFCEAGFEDVAVSRCSFAHLNLADPVFYLARKVEPFI
                     EENLPYLAYNVCAGGKKPEG"
     gene            10629..11174
                     /locus_tag="PLI76_04360"
     CDS             10629..11174
                     /locus_tag="PLI76_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOI61298.1"
                     /translation="MEPVDIVLTKDTFEVLASETRLTILKTLNERRMTITELADNLGL
                     AKSTIHHHLQKLTGAGFVAADGDGHAWVYYALTPEGRALLQPHGSARIRIILTAGLAS
                     LAGGVCALAAFLTAPVREGPVFPGPKGGGGLPVPEGAPVELLVAGVALAAVGGALMTY
                     ACVRWRNTRAAGYFPADEKSA"
     gene            11331..12623
                     /locus_tag="PLI76_04365"
     CDS             11331..12623
                     /locus_tag="PLI76_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOI61299.1"
                     /translation="MKGTIWLFSGISILILALILVGLTAAGTAPQNTTSISGKPVAGD
                     AGIAHPDTPDGAGNVAAGNNRFAFDFYRRLATDPAHAGDNLFFSPYGISSALAITCEG
                     ARGATADEIGAVLHLPTNETLRREGFAAIDTGLNCASGNYTLRTANALWAEKTHSFLP
                     EYIDVAVRWYGANVTNLDFVENPGGSRETINRWVEEKTEEKIRDLLPPGSIDSMTRIV
                     ITNAIYFKGTWAEQFDPDRTTGEEFRVGPNETVTVPMMHGKTIYPYAETDTLRVLEMP
                     YAHGNETELAMLVLLPKGDNLTAAEEALDTERLAGLQESLVSQNVRVFFPKFTLETGY
                     PLAGVLAAMGMPTAFSPDADLSGMDGTRNLFITGIYHKAFVDVNEEGTEAAAATGVIG
                     GKGVTPVFRADHPFVFLIVEKNSGTILFAGRVVNPESP"
     gene            complement(12688..13059)
                     /locus_tag="PLI76_04370"
     CDS             complement(12688..13059)
                     /locus_tag="PLI76_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOI61300.1"
                     /translation="MRDGIDRLLDIQEAIKEIERYAVRGRDAFLQDELVQVWMVHHLR
                     IIGEAAARLPPDLRSASPGVPWKQVIGLRNVLVHAYFRVDPEEVWAVVNRDIPALREE
                     VAMLLSLLGGTDGAGQPTIPS"
     gene            complement(13056..13349)
                     /locus_tag="PLI76_04375"
     CDS             complement(13056..13349)
                     /locus_tag="PLI76_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOI61301.1"
                     /translation="MPVTVDLIREKRSRILAIAGRHGARNLRVFGSVARGEAGPESDL
                     DLLVELEPGRSLLDHIALIQDLEEALGCRVDVVTEAALKERYKKRILKEAVPI"
     gene            complement(13561..14889)
                     /locus_tag="PLI76_04380"
     CDS             complement(13561..14889)
                     /locus_tag="PLI76_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOI61302.1"
                     /translation="MDVEEISRRHLAAGTPDDDIVHLLSRDILAIKHNRISSAYAGAF
                     ARAVLAEAKNSTGLSGDLFAFEPSGSTMGEFGVGSRGSGDFFAHRQLARIIGRTSAAV
                     GVDEMDDAGAVRAGGDYIITTVDGMHSRLSDFPFLAGFHVTRATLRDVYVMGAKPVAL
                     LSDIHVADDGDVAKIFDYTAGVSAVGEAMGVPLVTGSTLRIGGDMVLGTRMTGCVGAV
                     GVANHLTARKAIQPGDVLLMTEGAGGGTIATAAIYSGFPDVVDETINLHFLKACEALL
                     ASDVLNGIHAMTDVTNGGLRGDAYEMAETAGCRIVVEEDELRTLVQPKVLAMLDALEI
                     DYLGVSLDALLVVAPPEVAPDIRRVVASAGVAMKEVGYVEKGAPESVLSVGGEIRDFS
                     PRFRESAYTPVKKVVDEDKRDFAEMKAGVERAAEAALAKKLRILSRLRSS"
     gene            14986..15786
                     /gene="thiD"
                     /locus_tag="PLI76_04385"
     CDS             14986..15786
                     /gene="thiD"
                     /locus_tag="PLI76_04385"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HOI61303.1"
                     /translation="MTGAQTITACSIAGSDSGGGAGIQADLKTFTALEVYGLTVVTAV
                     TAQNTREVRGVWMLPADAVRTQIEAVVDGFSIGAWKTGMLGNTSNIRAVAEALPDGAV
                     LVIDPVMVSTSGHRLLDGDAVTDLVEQLIPRADIVTPNLPEAEVLGRMRVATLDDMTE
                     AGRRILDLGARAVVVKGGHLAGDAAVDIFIDRDGVVPLSGRRYPYSVHGSGCCFSAAL
                     AAYLARGMAARPAFAAAREFIDTAIREAAGGPGPLRIVNPGGTNLRRR"
     gene            15986..>16412
                     /locus_tag="PLI76_04390"
     CDS             15986..>16412
                     /locus_tag="PLI76_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOI61304.1"
                     /translation="MDEIDDAILRELQRDGRMSMAELGSMLGIAPSTVFKRIEKLKNA
                     GIIERFTIVINQKCFEHTLVAFLNIRVHPEGKPEVEAFVRDHQGILEVYEVLEPGDFF
                     VKVRVQNISELKRSVLIPLSSLPGVKEIQTIISVRKIKEQ"
BASE COUNT         3071 a         5218 c         5175 g         2948 t
ORIGIN      
        1 gcactttata tgtccatcag gtcgctcatt cccgtgacca caacgactcc accctgtcga
       61 gcgacccact gtcaccctct ttcccggact tacccgtcaa tctctggcat cgagcacacc
      121 aaggtcacgt gatccccggt tgatcacgcc attttttcaa aaaaacgtca atttaggcgt
      181 attttccagt tcttttttcg aaagctcccg ctctccctga cagaccgtgt tcgagccgga
      241 tgccctccgg tcgttcaggt caacgcttca ggatagcgtc tcgggccagc ttctttgggt
      301 ggtattggag accgtgacgg cagagtcgag cgaaccagag gttccatccc tctctgcccc
      361 gggcaaaaac gaatgagcga atacagagaa gcatcggggg cggtttaccg ggaagagcgc
      421 cggcgttgcg actcacggga aaaagtgccg atagagttct gcagagaccg gatgcatctc
      481 tccaggcttt ctgagtgatc cgacgcaaca tccggtctcc atccccgttc ggcatcggca
      541 acgaactctg tgaggagcga gaggtacgca gggccctcca cccgccggct gcccggcccc
      601 gggacgatgc ccttcctgat cctcgaggaa ctcgattccc gtgcacagtt gatcaggtat
      661 tccttcgggt aatcgatctc ttcgggcgtt ccggtgatgc gtgtctccgg aacctgcaga
      721 tatcttgcca gtctctccct atcggccagg atccaggctt caacctctct cacggccacg
      781 cggaagttca tgaagtacga aggggcgggc agccagttcc gaacaacttc cggcgcgcac
      841 ccggcatcgt gatccagatc gacgaccact acccagggcc gggcacgggc agaaacgttg
      901 aatccctgta acttctccag cagataggat ttcctctgcc tgtagagatc ccccggcagg
      961 gccccggcgt gccggagcag ccgtctcaca actgctccat ccagcgatcc ctcaaaagcg
     1021 cactgaaacg ttatcgcact gtccatccta ctccccaaac caggccagct ctgccgtgtc
     1081 gccgggacag gtcttcggga tgacgacatc tgcaagggat aggccacctt tgagcaggtc
     1141 gtcgatctcc tcgatgttgc cggccgacac aaccgtcgta ccctcctcag agggccggag
     1201 cagcagcacc tcatcgagcc cgatgccctc gtcctgcagc agttcggagg aatgggtact
     1261 tatgagaatc tggcggtcac tcttcaattg cacccgggag aagatctgcg ggacatacct
     1321 gattatcgca gggtggagcg agagttccgg ctcctcgaga aggagcggtc ctccgtcatc
     1381 cagcagcgcc cagagaatgc cgaggagcct gagtgttcca tcagagagct gctgctcggt
     1441 ttgccaggct tcctgcggct tccagtgttt atatctcccg tagaggtgcg gttttccaat
     1501 gctgtcgaac tcaaccttta tctcgctgag ttgtggcacc gcaacactga gcgccctctc
     1561 gatccggcgc agcatggaat cccgctcttc ttttgatctg cccatgacct gcttgaggaa
     1621 atcactgcca tacggatcgt tcctgtaacc aacagaccga tccggttcgc ggaccagttg
     1681 cgggacgagg tgcaggtagc gggtggtctt caggaaatca gcgacctccc tgaaatcttt
     1741 gttgacgttg acctgctcca ggtatgtctg ggttaacctt tcgggatcct cctcatcctg
     1801 cgcggaggga cgggcgatca cttcgttccc acaccttgca acccgttcct ttgcgataac
     1861 cggcaggtta cgctcgtcct gcctgaacgt cagctcgtat tcccagagat cgtcgcgctc
     1921 agcgttaccc agccgcacca ttatgcctat atcggaatac tttcgtgcag agagcgccct
     1981 gaggctggag acaccacctc gttctttcac cgctccctga aatccgccac ctacagatac
     2041 gatatcccgt aagaatttga atacgtccag gaagttggat ttcccggatg cgttgggtcc
     2101 gacaaggaaa acccgagcct gcaggtccat atcgaccctc tggaagttgc gccagttcca
     2161 gagagagatg tgggtaaatc tcttcggcat atccacgcat ctggagcagt tacccatgat
     2221 actgatcgat tgaagccccg tgtatattac cctttttgag gaccggaggt tccctcacgc
     2281 agtatgcccg gagataagag gctgatctgc aaccttcgcc ggcagagaaa ccaggctaaa
     2341 tatatcgtca ggtaggttga ataccacacc gatcgtcctg ttccattcga ctttgagaga
     2401 aacctatgag gatcttaaag attaccgtcg aggaacaccc tgacgcttat gtcgcctgcc
     2461 ccccgggtct gaagggtgtc gtcattgcag agggggacac ccatgaagag gcgcttgccc
     2521 acagtaatcc ggacagcaca acgatgccga accacccaca aatcaaaggc ctgactctgc
     2581 ggagcatctg cacacaggca gggattccgc gcgaagagtt cctgcgagcg tatgagcagg
     2641 agcaggcaat acagttccct gccttctccc cggcaagagc cccttattac cctgcaccac
     2701 aaacctgcta accatgatca ccgaccgcga gagagcggcg ttcgaagccg gtatcaagct
     2761 cggcgccctc taccaccagt tcgtcgggac gcccatcgcc cgcgagaccg ccgccggcgt
     2821 cgaggccgcc atcgagcagg ccgtcggcct ccagccctac gtgacggaga tccgcgtccg
     2881 gctgaactgc gatatgatgg tctcgaaccg gttcggctac tcggaacttt ccgggcggat
     2941 gtttgatgcc gagatcacga caacggtcgg cgcaaccgtc tgccgcgccc ggctgcatct
     3001 cgaggacgac taccccatga tggagattgt cgatatccat gaaacctccc gcgataccga
     3061 taactgacga ccacatccac atcgaccccg tgaacggccg gggcgtcgag gccgcaaagg
     3121 actttcgccg gagcgggggg acgcacatct tcctggtctc aaagccctcc tggtcgtttg
     3181 gggtcgagcc gtcctcgggc gaagactacc gggaggtttt tctcgcgacg ctccgtgtgg
     3241 ccgatatggt ccgggagacc gggcttgtcg tctacccggt cctcggcgtc cacccggcgg
     3301 agatcaaccg tctggcggcg cgtatgagtc ttgaagaggc cgccggcgtc atgatggccg
     3361 ggctcgacct tgccgcccgc tacgtcgagg gaggagaggc cgtcgccttg aagagcggtc
     3421 ggccccacta cgaggttcca ccggaggttc ttgccgcatc gaacgcgatc ctctatcgcg
     3481 cgctcgaact cggcgcagac tacgactgcg ccgtccagct ccacgccgag agcgggccct
     3541 gcaccgacgt cgtcgatatg gccggccggg ccggtatccc ggtcgagcgg gtcgtcaagc
     3601 acttcgcgac ccccgatacg ccgctcatgc cctccctcat cgcgcggcac gaagatatcc
     3661 cggcgctcgc ccgggcgggg cggcatttca ccatggagag cgactacatg gacgaaaacg
     3721 cccgccccgg cgcggtgatc gggccgaagt cggtcccgcg gttcacgcac cgctacttga
     3781 aagagggatt gatcacagaa gaggatgcct ggcgcatcca cgccgcgacc ccgtcgcgca
     3841 cttacggcgt ggatatcacg cttccctgac ggaactgcac acctttttga cgactcccgt
     3901 caaacgatat acgatgtact taaaagtgca ccacgtaccg gacgccggtg aggtggtggc
     3961 tgcctgcgac tccgagctcc tgaatgtcac cctgatgcac ggagacgtgg aggtctgcat
     4021 caccggagga ttctatggca cagaacgtgt cgctgaggag gaggtcaggc gtgctcttct
     4081 gggtgcgacg aacgcgaaca tcatagggaa gcgtgtcgta gccctggccg tcgccctggg
     4141 gctcgtcaga agggaagact gcatcatgat cggcgacgta ccccacgccc agatcttcgg
     4201 gatgtaattc atggccatac agacgagcat ctgcccccgc tgcgggcagc cgaccgaaga
     4261 ggggctctgc ccgcagtgcc gggccgcgga tacccggctg ctcgcctgcg agccccgcgt
     4321 gaccgcggtc tactgcccgg tctgcgactc gcagaggcgc ggcaagacct ggtcggatct
     4381 gcggatgccg cgcgaagacc tcatcgcaga gatggccata gcggcgacga gcatccatga
     4441 ggatgcaaaa gacatccggg tcgccgtccg gtcggcagag accgggccga acaggacgac
     4501 ctgcaccgtc gatgtggagg ggaccctcta ctccgtcccg gtccacggga cctgcgaggt
     4561 cgagatcctc tggcagaagg aggcctgcga tcggtgcagc cgcatatccg ggggctacta
     4621 cgagggaatc gtccaggtca gggctacgga cagaaagatc aatgcctacg agcgctccgt
     4681 cgccgcgagc atcgccgatc aggccgaaga gtccctccag caggcgggag accggctctc
     4741 gttcgtatcc gagctcgaag actcaagaga cggcgtcgac atcaccgtcg gatcccagca
     4801 cctcggccag gagatcgccc ggatgatcac gggggctctc ggcgggcggt atacgaccca
     4861 cccgaaactg gtcggcgaga aggacggtaa agccctctac cgcgtcacct actccatccg
     4921 ccttcctttc taccagaagg gggacgtggt ggtctcccac ggcggctact ttgaggtccg
     4981 cgatatcgac ggacgccggc tccgggcctt tgatcttgca accggcgcga cccggacgct
     5041 cgccgaggat gaggtcgagc gcctgatcgg gaacgttcgc gaggcggagt ccgcgctcgt
     5101 cgtcttcacg cagcccgaca tcgtcgggct gatggacccg aagacctacc ggacccggga
     5161 gcagagcccg gtgccctgga cgttcccggt cgaagggcag ccgatccggg tgctccgcga
     5221 cgaagagcag gaccgcctgg tcatcgtcgg gtgaggggcc ggcatgcgtg cgcggagggt
     5281 gccggcggcc gttcttgccg atatcgtggg tgcggagtgg gtggaccctg cccgccggcc
     5341 ctacgtccgg ggcggcaccg cctgggtccc ggtccgggag gggtatccgg ccgacgcgga
     5401 ccttcccgaa cgggagagct accgggggcg cggttaccac cggatcggcg atgtcgcggt
     5461 cctccgtggc gacgcgccgt cggaggctga gctcgccgcg atcgtggagc actgccgccc
     5521 ccggggcgtc ctcctggtga agggatacct gggcgatatg cgcatccccg atgtggagat
     5581 cctctacggg aacacgggcg aggtatgcca ccgcgagcag ggctacacgt tcatcctcga
     5641 cccggcccgg gtgatgtttg ctcaggggaa ccgcaacgag aaggcgagga tcgcctccct
     5701 cgtccggccc ggcgagcggg tcgccgatat gttcgccggg ataggctact tcaccatacc
     5761 ggcggccgca ggcggcgcgc aggtccatgc gatggagata aacccgatag cctttgaata
     5821 cctgcagcgc aatagtatgg ctaaccatgt tgctgaccgg gtcacggcag agaacgggga
     5881 ctcccggata ctcctctccg ggacctacga ccgcatcctg atggggcact tcgatgctcc
     5941 gtcgatgctc gccgacgccc tcgcccacgc ccacagcggg agcgtgctcc acgtccacag
     6001 catcggggat gtgagaggtg cgatccgcga cgcgacagcg gaagcgggct ttacggcgat
     6061 ggtgacctcc cgtcgggtga agaagtacgg cccgcatgcg tggcatatgg tgcaggatgt
     6121 gacgatatcg tgaagacgtt ttcgggaaaa acggtggtcc tcgccgtgac ggggagcatc
     6181 gcggctgtgg agacggtgaa acttgcccat gcgctgcgcc gccggggtgc tgaggtgcag
     6241 gcggtcatga cggaggcggc gaccgggatc atccaccccg atgccctgac ctacgcgacc
     6301 gggcggccgg ctataacccg tatcaccggc ctcgtggagc acatcctcta ctgcggcgag
     6361 ggcggggcgg ccgacctcct cctcatcgct ccctgcacgg caaacaccat cggcaagatc
     6421 gccgccggta tcgacgacac cccggcgacg accttcgcga cgacggcgct cggccggggg
     6481 atgccggtgg tggtcgctcc ggcgatgcac gagagcatgt accgccaccc cggggtcgtg
     6541 gagaacctcg aacggctccg ctcctggggg atcgacatcg tcgccccccg gatcgaggag
     6601 ggaaaggcga aggtcgcgga tatcgatacg atcgtcctcc acgccgcacg tgcagtctcc
     6661 ggccggccgc tcgcggggag acgggtcctc ataacgagcg gggcgtgcgc agaacccttc
     6721 gacgacgtcc gggtcctcac cacccggtcg accgggcgga tgggacgggc gctcgcgctc
     6781 gaggccttcc ggctcggcgc cgacgtgacg gtcgtgcacg cgggatcgtt tccctgcgtc
     6841 cgaaacatcc gtgtagcgac cgctgcagat atgcgccggg cagtcctctc ggtctgccgc
     6901 gacgacgggg tcgacatcta cgcgagcgcg gcggcgatat cggacttcgc ccccgagcgc
     6961 tgcgagggga agataccgag cggctcgccc ctcgacatcc ggctcaatcc cctcccgaag
     7021 atcatcgatg aggtgatggc gacgtgccgc cccgtgacgg tggcgttcaa gctgggctgg
     7081 cacgaggagg agcgagcgag aacgatgctc gatgcgggcg tgaagatggt cgtcgtgaac
     7141 gctccgccgg cgatgggggc ggcagggggg acgttctcgc tcatgatggc agatagtacc
     7201 atcgaggtaa cggggagcaa agaggaggtc gcggcggcga tatggtcggg actgctgtag
     7261 ccttctcccc cgggcacata tccggctact tcaggcgggt cgaggggagc gatccccgga
     7321 ccaccgggag cgtcggggcc ggcgtggtca tcgacgaagg ggtgcgcgcg acggtcgtcc
     7381 cggccgcaaa gaccggtgtc cgggtgatcc ggcagggccg cgtgagcacc ggatcgccgc
     7441 cggtcgagta cgccctcgac cggctggggg tcacggcccg cgtcaccacg gagtgccggc
     7501 tccccatcgg cgccgggttc ggcctctcgg cggccgcgct tcttgcgacg ctcacggcag
     7561 taaaccacct cttcgacctc ggcctctccg cggatgaggt ggcagcccgg gcccacgagg
     7621 ccgaggtcgt ccaccgcacc ggcctcgggg acgtcgccgc gagccggtcg gggggcgtcg
     7681 tctgccggat ggggcccggc atccacgcgg atatcgcccg attttacccg gacgaacccc
     7741 tctacgccgt gagtctcggc ccgctcccga ccgcctcggt cctctcgtcg gaggaagcaa
     7801 tggcgcggat agcctctgcc tacccggatc gctgcccccg cgatatcgcc gacttctgct
     7861 tcctctcacg gcagtttgcc gagagaagtg ggctcatcgc gccggatgtg cgaaaggtgc
     7921 tctctgcctg cgacgccgcg aagatcccgg caagcatgac gatgcttgga aacggcgtct
     7981 ttgctgcagg caattccgca gagcgcgtac tttcgggatt cggtgaggtc taccgcctca
     8041 acgtcgcacg ccgcggcgca tacttgatcg agatgagacc atgataccga aagatcatcc
     8101 acgctaccgc tccctcatcg cccgcgaacg gctggcacgg tgcgcccgcg agggtatcgt
     8161 cgccctcgag gggcttgccg cccacgggcg cggggaggcg tttgactacc ttatcggaga
     8221 acggacgagc gagagtgcag ctctcgccgc ccggacggcc gccgccatgc ttctctctgc
     8281 ccggcacccg gtgatatcgg tgaacggcaa caccgcagcg cttgcggcag atgagattgc
     8341 ggccctccag aaggcaagcg gcgcagatgt cgaggtgaac ctcttccacc ggacggagga
     8401 gcggatacgg ctgatcaccc ggctccttga agagcacggt gtccgggtcc tctccgggac
     8461 cgcggaacgg ctcgtcccgc tcgctcacga ccgggcgctc tcgctccggg aggggatcgg
     8521 agacgccgac gtggtcctcg tgccgctcga agatggggac cgctgcgcgg cgctccgggc
     8581 gatggggaag acggtcatca ccatcgattt aaacccgctc tcccggacag cccgcacggc
     8641 gaccctgacg atcgtcgatg aactgacgcg cgccgtccct gccatcaccg tggcgtgcgc
     8701 ctctctatcg cccggcgagg gcgagcgcct gatcgcctcg cttgacaata tctatcttct
     8761 ccgggcggcg atagatgaga tgagagagag attgagccat gctctggagt gaggtctacc
     8821 gcccgaagcg gtgtgaggat atcatcgggc aggatgcggt catccgttat ctcgtctcgt
     8881 ttgcggatgc aggaagcgtg ccccacatga tcctatcggg cccgcacggc accgggaaga
     8941 ccgtggccgt cgagtgcctg gcaaaacggc tctacggcga gaactgggag gcaaacacca
     9001 ccgtcctcag cgccgccgat ctcctcgggc gggggaggag cgcccttgag acggacgagc
     9061 gctttgccca gatctaccga aaggaccaga gcctgatcgt caacttcaag tatgtcgtga
     9121 aatggtatgc ctcgatgcgc ccgctcgatg ccgacttcaa actgatggtc ttcgaggatg
     9181 ccgaaggcct gacgtttgag gcgcagcagg ccctgcgccg caccatggag cgctacagcg
     9241 ccacctgccg gttcatattc gtcaccaccc ggccatcggc gatcatcccc gcgatcgcgt
     9301 cgcgctgcct gccgctcttc ttcgctccgc ttgagagcgc gctcatccgc acccgcctgg
     9361 cagagatcct cgccgcggaa gcggctacgt gcccggccga cgacatcgac ctgatcgtcc
     9421 acgctgcccg gggcgacctg cgacgggcga tcacgtacct ccagatcgtt gccggggcgg
     9481 gagaagagat cgaccttgca gaggtggcgc gctccgagac ctcgaatgtg gcaacatccg
     9541 cgtttgaagc gctccgggcc ggcaattttg ccgccgcgcg cagcatcgcc gagtcgctga
     9601 tgatcgagta cgggctcccg gcgcgggagg tcgtcggcga actccggcgg gcggtcaggc
     9661 gcgagtacaa ccaccctgcc cttgcgatcg cgctcgccga tgcggactgt cagctcggtc
     9721 acaatgcaaa cgattttgta cagatcaatg cacttcttgc ccggattgcc cgggaggtgt
     9781 ccagtgaaga aagtacagca gcactatgat gagatcgctg atatctacga cgatcgctac
     9841 gatggcaacc gcgggaagta ctatcacggg cacatatccg accacgtgat gggcgttctc
     9901 ccccagggcg ggttcctcct cgatatcggg tgcgggaccg ggcttttcgt gcaacgctac
     9961 gtcgaagaag ggggtcgggc ggtcggcctc gatataagcc cggggatggt ccggcacggg
    10021 cggcagcgct gcccggcgag cgggttcttt gtcgggaccg ctgacgtcct cccgtttaaa
    10081 gatgccacgt tcgatgctct tgcaagcctg ctcgccttca gttacgttcc cgacccggag
    10141 gcgatgctcc gggagtgcta ccggatctta aaacccggcg gccggatcgc cgtctgcacc
    10201 ctctcgcgga cggtcttcac gagcatcgtt cctatcgcct accaggtggg ggagaaggtc
    10261 ggcctgaaga aggtgggcgt cggggacttc ggcgagcact actacaccaa cagcgagatt
    10321 gccaggctct tttgcgaggc aggattcgag gatgtcgcgg tcagccggtg ctcgtttgcg
    10381 cacttaaacc tcgcagaccc ggtcttttac ctcgcccgga aggtggagcc cttcatcgag
    10441 gagaacctgc catacctcgc ctataacgtc tgtgcgggcg ggaagaagcc ggagggttag
    10501 atcactgtgt gggcgggatg cgggagtcac tccctgctcc cacccaccgg gccgcatggg
    10561 aaggctctgt tttacgatgc agagacgata acggaatagt tatatcctgc ctccccgaac
    10621 tcctccccat ggagccggtc gatatcgtcc tgacgaagga tacgttcgag gttctcgcat
    10681 ccgagacccg gctcacgatc ctgaagaccc tgaatgaacg caggatgacg atcacggagc
    10741 tcgcagacaa cctcggcctg gcaaagtcca ccatccatca ccacctgcag aagttgaccg
    10801 gcgccggttt tgtcgctgcc gatggtgacg ggcacgcctg ggtatactac gcgctcacgc
    10861 cggagggacg cgccctcctg cagccgcacg gcagcgcccg gatccggatc atcctgactg
    10921 cggggctggc aagcctcgcc ggaggggtct gtgccctcgc ggccttcctg accgcgccgg
    10981 tccgggaagg gccggtattt ccgggaccga agggcggcgg cggcctcccg gtcccggagg
    11041 gggcgcctgt ggaactgctc gttgccgggg tcgcgctcgc agcggtcggg ggggccctca
    11101 tgacgtatgc ctgcgtccgg tggcggaaca cgcgcgcggc ggggtacttc cctgcggacg
    11161 aaaaaagcgc gtagcctcaa tacatagctt ttgaaaccat tcgaccggga tcgaacggtt
    11221 tcgccggtat tatcgtcttt ctgcgagaga gaatccatgt ggatgcccgg ggtgtacccg
    11281 gtctcgaagg ttttcggcct tccccccgat gcgatccacg ggagagaacg atgaaaggaa
    11341 cgatatggct attctccgga ataagcatcc tgattctggc gctcattctt gtaggactga
    11401 ccgccgccgg caccgcaccc cagaacacga catcaatctc cggcaaaccc gttgccgggg
    11461 atgccggtat cgcccatccc gatacgccgg acggcgccgg caacgtggcg gccgggaaca
    11521 atcggttcgc gttcgacttc taccggcgcc tcgcgaccga cccggcgcac gcgggagata
    11581 acctcttctt ctcgccctac ggcatatcct cggctctcgc gatcacctgc gaaggcgccc
    11641 gcggcgcgac cgccgacgag atcggggcgg tgctgcacct cccgacaaac gagaccctcc
    11701 ggcgagaggg gtttgccgcg atcgataccg gtctgaactg cgcgagcggc aactacaccc
    11761 tccgcaccgc aaacgccctc tgggcggaga agacccattc gttcctcccg gagtacatcg
    11821 acgtggccgt tcgctggtat ggggcaaacg tcacgaacct cgacttcgtc gagaatcccg
    11881 gggggtcgcg ggagacgatc aaccggtggg tggaggagaa gaccgaagag aagatccgcg
    11941 acctcctgcc gccgggttcg atcgactcca tgacccggat cgtgatcacg aacgcgatct
    12001 acttcaaagg cacctgggcc gagcagttcg accccgacag gacgaccggg gaggagttcc
    12061 gggtcggacc gaacgagacg gtgaccgtcc cgatgatgca cgggaaaacc atctatccgt
    12121 acgccgagac cgataccctc cgggtgctcg agatgcccta cgcgcacgga aacgaaaccg
    12181 aactcgcgat gctcgtcctc ctcccgaagg gagataacct gacggctgcg gaggaggccc
    12241 tggacacgga gaggcttgcc ggcctgcagg agtccctggt ctcacagaac gtcagggtct
    12301 tctttccgaa gttcacgctg gagaccgggt atcctctagc gggagtgctt gcggcgatgg
    12361 ggatgccgac ggcgttctcc cccgatgccg acctctcggg gatggacggc acgagaaacc
    12421 tcttcatcac cgggatctac cacaaggcgt tcgtggacgt gaacgaggag ggcaccgagg
    12481 ccgcggccgc gaccggggtc atagggggga agggtgtcac acccgtcttc cgtgcggacc
    12541 acccgttcgt cttcctcatc gtcgagaaga actccggcac gatcctcttc gcaggaaggg
    12601 tcgtcaatcc cgaaagcccg tgaacgagag gctgttctct ccccttttgt cggcaacgga
    12661 taccggggca actgtcgcct acccgtttca tgaagggatg gtaggttgcc cggcgccgtc
    12721 tgtgccgccg agcaaagaga ggagcatcgc gacctcttct ctgagtgcgg ggatatcgcg
    12781 gttgacgacc gcccagactt cctcagggtc cacgcggaag tatgcgtgaa ccagaacgtt
    12841 ccgaagaccg atcacctgtt tccagggaac gccgggagaa gcgcttctaa ggtcaggggg
    12901 gaggcgggcg gctgcttccc cgatgatccg gagatggtgg accatccaga cctgcaccag
    12961 ttcgtcctgc aagaaagcat ctctgccccg gacggcatac cgctcaatct ccttgatcgc
    13021 ttcctggatg tcgaggagcc ggtcgatccc atccctcata tcggcactgc ctctttcaag
    13081 atccgctttt tgtaccgttc cttgagcgcc gcttccgtca ccacgtccac ccggcacccc
    13141 agggcctcct ccaggtcctg gatgagtgcg atgtgatcaa ggaggctcct gcccggttcc
    13201 agctcgacaa ggaggtcgag gtcgctctcc gggcctgcct cgccccgggc gaccgacccg
    13261 aagacccgga ggttcctggc accgtgtcgc ccggctatag cgagaatcct ggaccgcttc
    13321 tcccggatta gatccactgt gacaggcatg gctgttcaat atcttctatc gggagctggc
    13381 aatataaatc tgccctcaat ggtcgcaccc ctaaatagat cccggacata ccccgaacta
    13441 tggtcttccg aagagggtga aatccggccg gcacccccgc cttcttgtac tgctgattgg
    13501 gaatcgatgc actaaagaga gaaagtatct ctccatcgct caataagccg ccctgtttcc
    13561 tcacgacgac cggagccggg agaggatccg gagcttcttt gcaagcgccg cctccgccgc
    13621 ccgctcgacc cccgccttca tctccgcaaa gtctctcttg tcctcgtcca cgaccttctt
    13681 gaccggggtg taggccgact ccctgaatcg gggcgagaag tcgcggatct cgccgccgac
    13741 cgagaggacc gattccggtg cccctttctc gacgtagccg acctctttca tcgcgacacc
    13801 tgcggacgca acgacccggc ggatatcggg cgcgacctcg ggcggtgcga cgacgagaag
    13861 cgcatcgagc gatacgccga ggtagtcgat ctcgagcgcg tcgagcatgg caaggacctt
    13921 cggttgcacg agcgtgcgga gttcgtcctc ctcgacgacg atccggcacc cggcagtctc
    13981 cgccatctcg taggcgtcgc cccggaggcc gccgttcgtg acgtcggtca tggcgtggat
    14041 gccgttgagg acgtcgcttg caagaagcgc ctcgcatgcc ttgaggaagt ggaggttgat
    14101 cgtctcgtcg acgacatcgg ggaaccccga gtagatcgcg gcggtggcga tcgtcccgcc
    14161 gccggccccc tcggtcatga ggaggacgtc gccgggctgg atcgccttcc tcgcggtgag
    14221 atggtttgcg acgccgacgg caccgacgca ccccgtcatc cgggttccaa gcaccatgtc
    14281 cccgccgatc cgtaacgtgg atccggtgac gagagggacg cccatcgctt cgccgacggc
    14341 ggagacgccc gccgtgtagt cgaatatctt cgcgacgtcg ccgtcgtcgg cgacgtggat
    14401 atcggagagg agggcgacgg gttttgcccc catcacgtag acgtcgcgga gcgtcgcccg
    14461 ggtgacgtgg aacccggcga ggaaggggaa gtcggagagg cgggagtgca tcccgtcgac
    14521 ggtggtgatg atgtagtccc ctccggcacg gacggcgccc gcatcatcca tctcgtcgac
    14581 accgaccgcg gccgacgtcc tgccgatgat ccgggcgagc tgccggtgag cgaagaagtc
    14641 gcccgatccc ctcgatccga caccgaactc acccatcgtc gagccggaag gctcgaacgc
    14701 aaagaggtcg ccggagaggc cggtcgagtt cttcgcctcg gcaagaaccg cccgggcgaa
    14761 cgccccggca taggcggagc tgatgcggtt gtgcttgatc gcaaggatat cccgggaaag
    14821 aaggtgcacg atatcgtcgt caggagtgcc ggcagcaagg tgccggcggc tgatctcttc
    14881 tacatccatt gtaagagtga tcggtgccgg gagagataaa aggagtggcg gcggggctct
    14941 ccctgcgggc aaagattcaa gcggatccgc cccgaataca gatggatgac gggtgcgcag
    15001 acgatcaccg catgctccat agccggttcc gattccgggg gaggtgcggg catccaggcg
    15061 gacttgaaga cgttcacggc gcttgaggtc tatgggctga cggtcgtaac ggcggtgacg
    15121 gcccagaaca cccgcgaggt ccggggggtc tggatgctcc ccgccgatgc ggtgcggaca
    15181 cagatcgaag cggttgtcga cggtttctcc atcggggcgt ggaagaccgg gatgctcgga
    15241 aacacctcga acatccgggc cgtcgcggag gccctgccgg acggtgccgt gctcgtcatc
    15301 gacccggtga tggtctcgac gtcgggccac cgtctgcttg acggagatgc cgtcacggat
    15361 cttgtcgaac agctcatccc ccgggcggat atcgtcaccc cgaacctccc ggaggcggag
    15421 gtgctcggcc ggatgcgggt tgcaaccctg gacgatatga ccgaggccgg tcggcggatc
    15481 ctcgacctcg gcgcaagggc cgttgtggtg aaaggggggc accttgccgg cgatgcggcg
    15541 gtcgatatct tcatcgaccg tgacggggtg gtcccgctct ccgggcgccg ctatccctac
    15601 tcggtgcacg ggtcggggtg ttgcttctcc gcagcgctcg ctgcatacct tgcccgggga
    15661 atggctgcgc gccctgcgtt cgccgcggcc cgggagttca tcgatacggc catccgggag
    15721 gcggcggggg ggccggggcc actccggatc gtcaacccgg ggggaacgaa ccttcgccgg
    15781 cgttgaaata ataattctca cgtagggtga atttccggcg attttttccc atatttgccc
    15841 gagaaatctg attttaccac cagataggag catgttaaaa tccccatata accggcatta
    15901 tccagtagaa acaggaaata ccctgttgaa gacttaaaaa caaatgcgta atcttaataa
    15961 ccaaaaacca gtaagtactg ttagaatgga tgagatcgac gatgcgatcc ttcgggaact
    16021 gcaaagagac gggcggatgt ctatggcaga gctcggttcg atgctcggta tcgcgccgtc
    16081 gacggtcttt aaacggatcg agaagctgaa gaacgccggt atcatcgagc gattcacgat
    16141 cgtcatcaac cagaagtgtt ttgagcatac tctggttgct tttctgaaca tcagggtcca
    16201 tccggaagga aagccggagg tcgaggcgtt cgtgcgagac caccagggta tactcgaggt
    16261 ctacgaggtg ctcgaaccgg gggacttctt cgtcaaggtc cgggtgcaga atatatccga
    16321 gttgaagcgg agcgtgctca tccctctctc cagcctcccg ggggtgaaag agatccagac
    16381 gatcatctcc gtgcggaaga tcaaagaaca ga
//
LOCUS       DAOSEE010000037        15952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1483_length_15952_cov_226.194628, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000037 DAOSEE010000000
VERSION     DAOSEE010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 15952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15952
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1483_length_15952_cov_226.194628"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..379
                     /locus_tag="PLI76_04395"
     CDS             122..379
                     /locus_tag="PLI76_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61305.1"
                     /translation="MLGDTQTMQQQAVMGEIEEMIQSMPDYAEVREALRSLDFIPEQD
                     DADVAIWVQPDLRIFVLLRMNLGGGYAGYKIAAYDEADTAR"
     gene            425..691
                     /locus_tag="PLI76_04400"
     CDS             425..691
                     /locus_tag="PLI76_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61306.1"
                     /translation="MHRQVTIDREEQERVRARVREVIATSPAYGDIRAGLEHLGFQAK
                     EDNPALALWENGENELFVMVHMDPDTGRLRDCEVKTFEEMEGFE"
     gene            complement(695..1189)
                     /locus_tag="PLI76_04405"
     CDS             complement(695..1189)
                     /locus_tag="PLI76_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61307.1"
                     /translation="MNVAMRRHFHIGGAPFLSGMGRKGSLAGILVSRLTGIILFLILI
                     GVLNVFADVYVGNPVFLRVVAFLNANVGFLILIAVIFLLGDLFCTLVFPLNLPGPIFG
                     ALGAVFVVAFIFRVFMLAGDMTGIEVFRIFSGTLAHLIYVLVFAAVLIGDYISLFSEP
                     SGRA"
     gene            1224..2966
                     /locus_tag="PLI76_04410"
     CDS             1224..2966
                     /locus_tag="PLI76_04410"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HOI61308.1"
                     /translation="MKFATFAACSEELGATSSRNDMTTLLARLLATAAPDDIAVVCYF
                     TLGDIAPGYSGVTTGIGDRTVAAAIARAARTTPLSVEAAARETGDYGDAAARLAGAGP
                     AAEPLTVAAVHRGLMDIARAAGPGSAERKVETLAALLSAATPPERRSIVRLATGKMRL
                     GAGDMTLLDALAEAYLGSKDARAPLEHAYNISSDIGLVARTLKSGGLPAVEAISVTLH
                     RPVRPMLTQRVARLSEIPERIGSTAIAVEEKYDGERVQAHKDGDDVTLFSRRLTDVTH
                     QYPDIVRSVQEHVTAGTVILDGEAVAFDHETKTYRPFQTLMRRRRKHRVGEVAREIPA
                     TYRAFDLLYMDGASYLRRSYPDRRAALEAVLSPDENISPADRIVTGDLNAITGFYIAC
                     IERGLEGIVCKSCADDSFYRAGAREWQWIKWKSDYAPDLADTLDLAVVGASAGRGKRA
                     GTFGSILCAAYNPEEDIFQTVCAVGTGFTDADLADLSRRFAGATVDRQPARVMATPQA
                     APDFWFVPADVAEVRGLEITESPVHTCNWDPERRRGLALRFPRFVRWRDDRSLEAATT
                     AAEVAAMFRRRRGA"
     gene            2970..4703
                     /gene="polX"
                     /locus_tag="PLI76_04415"
     CDS             2970..4703
                     /gene="polX"
                     /locus_tag="PLI76_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HOI61309.1"
                     /translation="MEMLPGMTNLEVAAILHEVADLLDIKGVRFKPHAYRRAAMAIET
                     LPEDVADVAREGRLGDIPGVGRGIAEKVEEIVKTGRLAYLESLRRELPAGVQELIRIE
                     GIGPKKATVLTRDLGIRSIDDLEAAAKAGRIRDLPGFGEKTEQNILAGIRMSRTAKER
                     YLLGYILPIAREIEERLRGLDAVRQVSLAGSTRRRKETIGDLDILAASARPEEVMEAF
                     VALPGVVRVLARGTTKTSVVLATGIQVDLRVVEERQFGAALQYFTGSKEHNIALRKLA
                     IARNWRLNEYGLVDLADNRTIAGEDEAGVYRALGLAWIEPELREDRGELEAARTGTLP
                     DIVGYDAIRGDLHVHTRWSEGVHSIGDMAEAATALGYEYIAICDHAETLHIARGLSPE
                     RLADQMREIEQINREGGSVTVLAGTECNIGMDGSVDLPDSVLADLDVVVASVHSGFKQ
                     PERAMTERVVTAMHNDHVDIIGHPTGRILLTREPYRIDIAAVAKNAAVLGVLLEIDAF
                     PGRLDLSDVNARLARDAGARFSLGSDAHNRENLRYMEFGVATARRGWLTADDIANTRP
                     LAGLRELLTRR"
     gene            complement(4690..5127)
                     /locus_tag="PLI76_04420"
     CDS             complement(4690..5127)
                     /locus_tag="PLI76_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOI61310.1"
                     /translation="MVQAKEGDTVKVHYTGKLEDGTVFDTSGDQDPLEFTIGGGQVIP
                     GFEQAVVGMEPGETKTATIPAEEAYGPHREDMTLTVDRSQFPEEINPEPGQQLQVQQP
                     DGRAAIVVVSDVSESTVTLDANHPLAGHPLTFDIQLVDLVSAG"
     gene            complement(5241..5864)
                     /locus_tag="PLI76_04425"
     CDS             complement(5241..5864)
                     /locus_tag="PLI76_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOI61311.1"
                     /translation="MYRERAPKDPSRVVTSDGVTFRRMPDGADAVYVVGEYHFDDIGP
                     EDIVLDIGANVGAFCIRAARRSRRVLAVEPVLAGALQENVRENGVSVTVVSGALGGGG
                     DERITWGGRTLTVATRTLGEFAGMIGGCDFLKCDCEGAEWSIRPADLDGVRRIEMELH
                     VPPIGGPVNRRLLDYIGEHYDFTIDRTPGYAALGVMGVLHAARRSGD"
     gene            6137..7396
                     /locus_tag="PLI76_04430"
     CDS             6137..7396
                     /locus_tag="PLI76_04430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOI61312.1"
                     /translation="MAYMDGITCICGNGRTLEDHRPIVGNDVISGIYRKAAGLRGKRV
                     LHVNSTYQSGGVAEMILSLVPLMNDVGIRTQWNILNGEGDFFVITKNFHNALQGQAIA
                     FSDEEKTLYLRTNETFSQRMAIDHDLVVIHDPQPLPLIRFYEKTQPWVWRCHVDLSNP
                     DPALWEFLKGFALDYDRMVISHEQYRRSGMPMEQWICRPAIDPLSEKNRDLSDAEIAG
                     LLAKYGVPTDKPLVTQISRFDKWKDPEGVVDVFCEVRRHVDCRLVLCGSMAPDDPEGR
                     AIYRRVEEAARPLIDAGDVVLITAEDHLLVNALQRASAVILQKSLREGFGLTVTEGLW
                     KGRPVIASRVGGIPLQIEDGETGFLLDPTDTEGFAWRIRQVLENPELGERLGRAGKEH
                     VRQHFLITRLLSDYLDMLNAELIGRNA"
     gene            complement(7901..9460)
                     /locus_tag="PLI76_04435"
     CDS             complement(7901..9460)
                     /locus_tag="PLI76_04435"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOI61313.1"
                     /translation="MENNITFKEFNISQKMLRAIEDLGFEEPTPIQVSTIPAILEGRD
                     VTGQAQTGTGKTAAFGIPAIERIDPESRETQVLVLSPTRELAIQIAEEFARLAKHHRG
                     INILPIYGGQPIERQFKGLQRGAQIVVGTPGRVLDHLDRGTLSFAAVNLVVLDEADQM
                     LDMGFREDIEKILDETPEGRQTVLFSATLPKPILEISKRFQKDPEFISVARREVTVPQ
                     IEQLYLEVRSRDKLEILSRLLDMYDPDLTLIFTNTKRGVDDLTTHLQARGYFAEGLHG
                     DMKQTLRDRVMAKFRAGSIDILVATDVAARGIDVEDVDLVINYDVPQDIEYYIHRIGR
                     TARAGRAGRAVTFVGPKEYFKLRTIQNYTRIQIARIPLPTPGDVEESRTRKLIDRVQQ
                     VIDEGSLERYVNLVERITSEDYTSLEVAAALLKLELGAGGQGPAQETGLALLRIPLGR
                     EHQVRPKDIVGALAGETGIPGGAIGAINIYETYSTVEVPQGAVDQVIEGMKGKTIAGI
                     RLPGPIETAGR"
     gene            9556..9993
                     /locus_tag="PLI76_04440"
     CDS             9556..9993
                     /locus_tag="PLI76_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61314.1"
                     /translation="MAARYPPPVSAADMREVYRRYAADRPGLRGILLPDLLPHDACGA
                     SPAPGRIRVLFVAEPPPWAAGPSDCLSPDYPYYYDDRYDAGRKRRSPAVLRRTSLLCS
                     ASTAGRAARTWRPSPGRDATSSTRSGASSERTEKRPYRPTSSG"
     gene            complement(10254..10949)
                     /locus_tag="PLI76_04445"
     CDS             complement(10254..10949)
                     /locus_tag="PLI76_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOI61315.1"
                     /translation="MAWRRMAREPWGEFDDMIANMQRQFEETMGRISGAAQQVALPGG
                     IRTTVDVREDETDVLVVADLPGVERQDISVRLTDPRTLRITARREMIHEGEETGYYVR
                     ERRVGAIARTVSLPADVTGEGSATFRNGVLEVRLKKIAEARGREIEVGGTSGKAALSA
                     AEEQRQQKEKLYREDREKVEPSGYLDPREIAKEARDIQLEEPGSPEERSTAARLRKRK
                     EELYAEGKKKLAG"
     gene            11145..14912
                     /locus_tag="PLI76_04450"
     CDS             11145..14912
                     /locus_tag="PLI76_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HOI61316.1"
                     /translation="MFRFNLSPSLIGRFFYHDCERYLRYHATPEQERENAGIPAIAVD
                     TSPTTRALLDAGIRWEEEVVRTRLSGRVRVPGGTGPISERSFSIEESFGLIPSLSPGD
                     AIYQTTIPVSVHFLKGYGLDPDMHRFSPCRPDLIRVDEEGRLSVIDIKASEDLSVSHR
                     VQATLYALILDHALDLLGISVPVDLSRAGIWLYGKDEPEYFDLSLNIRVIEDFFRHRL
                     PGILAGPPEDVPWHITPRCEACEFYPHCRAGAEASSSVSLIPGLSPTARRYLREAPWD
                     GGFSINTLADLAAFLRDPASDRHLDDCGSLAGRGDRLRATVRALATGEVVRLAGTSLA
                     LPVYEDIAIILSLQKDPVSGGVYALGFRRSRGEAVYGTPSHEAIYVAADEGDCARVRQ
                     EFIRALAAELAAVHDYNQGREWADQKSVQTYVYDATEGELFARSIREGLSDPATAEDA
                     LRLQFTYQETDIARGSSHPAGSVAFPLVVITGEIRRMLALPAPFALRLPEVLAIIPSS
                     RFAYTLSASSLFWNELSNAMKGDAVIMAWSGRAEAAGWIRQEVSRRLLAAGSILDGLR
                     ERVKAGLVRWPENFRFPRPWDAATPELSRLLFVTEYESTLAARRMQELRSRPWPVRVR
                     EGISIPLEKSEGNFWKVRTPLDLALFEQSQAFGHLLVPDGDAGEEAQLGFDDLRYRDS
                     PNPGKSGVSFARVHDSIVDREAGIVRGLVLTVNVGRDRTPFVEGDRAVLHPRFTDFTA
                     PRYVDRLLALDRQPENTFVRLLRDPRGFAAPVAETEAVIADAEQTVLDAGFTKSQARA
                     FHRVMTGRLTLVWGPPGTGKTHFLATAILALVRARRAHGERIRVGVTAFTHAAIENLL
                     VRVQDSVDEFGLAAGFPIYKLKDARTTRGERSLEVLPHDRADTVVGYPALLLGGTVHS
                     FERLEKSLPSLDLLIVDEASQMRPADLAMVLPLLADGGRLVLAGDDLQLPPVIQGEYP
                     EPEDGLPGLWDSIFAYLRHRDDPGRYTCQLQENWRMNRTLSRFPAETLYGAGYAPAND
                     AVGEQRIALAPGLPGEEWIAWALDPAYPLVLCILENVRTTVENQVEAGLVARLACTLR
                     ERLLDPASGNPYPATADGDALFWRRGLFVVSPHHAQIGAIRDCLSRERAWESRPFVDT
                     VDKMQGQEAEAVIVSYGVSDVETALSEAGFIYSRNRLNVSLTRGRSKCVVFLPRPLLE
                     PPLDLVGDPTAAAGLRHMLDLQEFCRTHGEEKTFSGDARGVRLTVMCARVE"
     gene            14955..15839
                     /locus_tag="PLI76_04455"
     CDS             14955..15839
                     /locus_tag="PLI76_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOI61317.1"
                     /translation="MIGSLDPDQNEREQRILKETREHGATPEAVRLFRALVLAHYRAH
                     GRDLPWRQTTDPYRILVSEIMLQQTQVERVAVKYPEFIDRFPDFASLARAPQSDILLA
                     WQGMGYNRRALALKRTAERVVDDYGGRLPADVETLATFPGIGRATAAAICAYAFNMPV
                     AYIETNIRRVFIHFFFQDREGVRDDEILVLVEQALDRENPREWYGALMDYGSILKKRT
                     ANPNRRSASYSRQSRFEGSDRQIRGRLLALVLAEGAVTGEEAAARVAADPGRARRILD
                     DLVREGFVAESEGTYTCR"
BASE COUNT         2694 a         5392 c         5215 g         2651 t
ORIGIN      
        1 gccgcccctc gtattccccc ggagaaagaa gagtcgccgg cagccccgtg cgagccgata
       61 ccacgccacc cgccttcacc cccggatcac ctttatgccg gatgccggca acggggggcc
      121 catgctcggc gatacccaaa caatgcagca gcaggcggtg atgggcgaga tcgaggagat
      181 gatccagtcg atgcccgatt acgccgaagt cagggaggct ctccgctcgc tggacttcat
      241 accggagcag gatgacgccg acgtcgcgat ctgggtccag cccgacctca ggatcttcgt
      301 cctgctccgg atgaacctcg ggggcggata cgcgggctac aagattgccg cctacgatga
      361 ggccgacacc gcccggtgat cgccgcaggt caggtttatc ccggcgtacg cagtaggggg
      421 agccatgcac agacaggtaa ccattgaccg ggaggagcag gagagggtga gggcccgggt
      481 ccgggaggtc atcgccacga gccctgccta cggcgatatc cgggcgggcc tcgaacacct
      541 cgggttccag gcaaaagagg acaaccccgc cctcgccctc tgggagaacg gcgagaacga
      601 actcttcgtc atggtccaca tggaccccga caccggccgg ctgcgggact gcgaggtgaa
      661 gacgtttgag gagatggagg ggttcgagta acgatcacgc cctgccggac ggctccgaga
      721 agagcgatat gtagtcgccg atgaggacgg cggcgaagac gaggacgtag atcaggtgcg
      781 caagcgtccc cgaaaagatc ctaaaaacct cgatgcccgt catatcgccc gcgagcatga
      841 agacccggaa gatgaaggcg accacgaaga cggcgccgag ggcgccgaag atcggccccg
      901 gcaggttcag ggggaataca agggtgcaga agaggtcgcc gagcagaaag ataaccgcga
      961 tcaggatgag gaacccgacg ttggcgttga ggaacgcgac cacgcggaga aagaccggat
     1021 tcccgacata aacgtcggcg aaaacgttca ggacgccgat caggatcaaa aagaggatga
     1081 tcccggtgag cctcgatacg aggatgccgg caagtgatcc cttcctgccc atcccggaga
     1141 ggaagggcgc cccacctata tggaagtgtc ggcgcatcgc aacgttcatc tctctcccgg
     1201 ccgtgatcac tcatgagagc cccgtgaagt ttgcaacctt tgccgcctgt tcggaggaac
     1261 ttggtgccac gtcgtccaga aacgacatga ccaccctcct tgcgcgtctg cttgccacag
     1321 cggcgccgga cgatatcgcc gtcgtctgct acttcacgct cggcgatatc gcccccggct
     1381 acagcggagt gaccacgggg ataggggacc ggacggtggc ggcggccatc gcccgtgcgg
     1441 ccaggaccac acccctctcc gtcgaggcgg cggcccggga gaccggagat tacggtgacg
     1501 ccgccgcccg actggccggg gcaggccccg ccgccgaacc cctcacggtc gcggccgtcc
     1561 accggggcct catggatatc gcacgggcag ccggcccggg atcggccgaa agaaaggtgg
     1621 aaacccttgc cgcgctcctt tctgccgcga cgccgcccga gcggcgctcc atcgtccggc
     1681 tcgccaccgg gaagatgcgg ctcggcgccg gggatatgac gctcctcgac gcgctcgcgg
     1741 aggcatacct gggctcgaag gatgcccgcg cccccctcga acacgcctac aacatatcct
     1801 ccgacatcgg gctcgtggcc cggacgctca aaagtggagg cctcccggcc gtggaggcga
     1861 tatccgtcac cctgcaccgc cccgtaaggc cgatgctcac ccagagggtc gcccggctat
     1921 cggagatccc ggaacggatc ggctcgacgg ccatcgccgt cgaggagaag tacgacggcg
     1981 aacgggtcca ggcgcacaaa gacggcgacg acgtcaccct gttttcacgg cggctgaccg
     2041 acgtcaccca ccagtacccc gacatcgtcc gaagcgtgca agagcacgtc acggccggca
     2101 cggtgatact cgacggcgaa gcggtcgcgt tcgaccacga gaccaaaacg taccgccctt
     2161 tccagacgct catgcgccgg cgccggaagc accgggtcgg agaggtcgca agagagatcc
     2221 ccgcgaccta ccgggccttt gacctcctct acatggacgg cgcatcatac ctccggcgaa
     2281 gctacccgga ccggcgggcg gcgctcgagg cggtcctctc ccctgacgag aatatctccc
     2341 ccgcggaccg catcgtgacc ggcgatctca acgcgatcac ggggttctat atcgcctgca
     2401 tcgagcgggg gcttgaaggg atcgtctgca aatcgtgcgc agacgactcg ttctaccgcg
     2461 cgggagcgcg ggaatggcag tggatcaagt ggaagagcga ttacgccccg gatctcgccg
     2521 atacgctcga cctcgccgtc gtcggcgcca gcgccgggcg cgggaagcgg gccgggacgt
     2581 tcggctcgat cctctgcgcc gcctacaacc ccgaagagga tatattccag acggtctgcg
     2641 ccgtagggac ggggtttacg gacgcagacc tcgccgacct ctcccggcgg tttgccggcg
     2701 caacggtaga ccggcagccg gcccgggtga tggcgacacc gcaggcggcg cccgacttct
     2761 ggttcgtgcc ggcagatgtg gcggaggtgc gggggcttga gatcaccgag agcccggtcc
     2821 acacctgcaa ctgggacccg gagcggcggc gggggcttgc cctccggttc ccgcggttcg
     2881 tgcggtggcg ggacgacagg agccttgaag cggcgacgac ggccgcagag gtcgcggcca
     2941 tgttccgccg ccggcggggg gcgtaaggca tggagatgct ccccggcatg accaacctcg
     3001 aggtcgcggc gatcctccac gaggtcgccg acctcctcga tatcaagggc gtccggttca
     3061 aaccgcatgc ctatcggcgg gcggcgatgg cgatcgagac cctgccggaa gatgtcgccg
     3121 acgtcgcgcg ggaggggcgg ctcggcgata tacccggtgt cggccggggc atcgccgaga
     3181 aggtcgagga gatcgtaaag accgggagac tggcatacct tgagtccctg cgccgcgagc
     3241 tcccggcagg cgtgcaggaa ctcatccgga tcgaggggat cggcccgaag aaggcgaccg
     3301 tgctcacccg ggacctcggt atccggtcca tagacgacct ggaggcggcg gcgaaagcag
     3361 gccggatccg cgacctcccg gggtttgggg agaagaccga acagaacata ctcgccggca
     3421 tccggatgag ccggacggca aaagaacgct acctgctcgg gtacatcctc cccatcgccc
     3481 gggagatcga ggagcgcctc cgggggctcg acgcggtccg gcaggtgagc ctcgcgggct
     3541 cgacacggag gaggaaggag acgatcgggg acctcgatat cctcgcggca tcggcacgcc
     3601 cggaggaggt gatggaggcc ttcgtcgcgc tccccggcgt cgtgcgggtc cttgcccggg
     3661 ggacgacgaa gacgtcggtg gtgcttgcga ccggtatcca ggtcgacctc cgggtggtgg
     3721 aggagagaca gttcggggcc gccctccagt acttcacggg ttcaaaggag cacaacatcg
     3781 ccctcagaaa actcgcgatc gcgcggaact ggcggctcaa cgagtacggc ctcgtggacc
     3841 tcgcggacaa ccgcacgatc gcgggggagg acgaggccgg cgtctaccgg gcgctcggcc
     3901 ttgcctggat agagccggaa ctcagggagg accgcggcga gctcgaggcc gcccggaccg
     3961 ggaccctgcc cgacatcgtc ggctacgacg ctatccgcgg cgacctccac gtccacaccc
     4021 ggtggagcga gggcgtccac tccatcggcg atatggcaga ggccgcgacg gcgctcggct
     4081 acgagtacat cgccatatgc gaccacgcgg agaccctcca tatcgcccgc ggcctctccc
     4141 ccgagcgcct ggccgaccag atgcgcgaga tcgagcagat caaccgcgaa ggcggcagcg
     4201 tcaccgtcct cgccggcacc gagtgcaaca tcggcatgga cggcagcgtc gaccttcccg
     4261 acagcgtcct tgccgacctc gacgtcgtcg tggcgagcgt ccactcgggg ttcaagcagc
     4321 ccgagcgggc tatgacggaa cgggtcgtca cggcgatgca caacgaccac gtcgatatca
     4381 taggccaccc gacggggagg atcctcctta cgcgggagcc ctaccggatc gatatcgccg
     4441 cggtcgcgaa gaatgcggcg gttctcggcg tcctcttaga gatcgacgct ttcccgggca
     4501 ggctggacct ctccgacgtc aacgcacgtc tcgcgcggga tgccggcgcc cggttctcgc
     4561 tcggctcgga tgcccacaac cgcgagaacc tccgctacat ggagttcggc gtcgcgaccg
     4621 cccggcgggg atggctcacg gcggacgata tagccaacac ccgcccgctt gcgggtctgc
     4681 gggagctcct cacccggcgc tgacgagatc cacgagctgg atgtcgaacg tcaggggatg
     4741 cccggccagg gggtggtttg cgtccagcgt cacggtcgat tccgagacgt cgctgacgac
     4801 gacgatggcc gccctgccgt ccggctgctg gacctggagc tgctggcccg gttcggggtt
     4861 gatctcctcg gggaactggc tccggtcgac cgtgagcgtc atgtcctcgc ggtgggggcc
     4921 gtaggcctcc tcggccggta tcgtggcggt cttcgtctcg cccggctcca tgccgacgac
     4981 ggcctgttcg aacccgggta tgacctgtcc tcccccgatg gtgaactcga ggggatcctg
     5041 gtcgcctgac gtatcaaaga ccgtgccgtc ctccagtttt cccgtgtagt gcaccttgac
     5101 ggtgtcgcct tcttttgcct gcaccatgct gcttcaccgg cggggtggtg aacaggaatc
     5161 ctatttatgc atgccggccg gacccgaaca aaccaggatc gtgcgccttt ccggtgacag
     5221 aaacggcggc cggcgatcgg ttagtctccg ctccgccggg cggcatgcag gacgcccatg
     5281 acgccgagtg cggcgtaccc gggcgtccga tcgatcgtga agtcgtagtg ctccccgatg
     5341 tagtcgagga gcctgcggtt caccggcccg cctatcgggg ggacgtggag ctccatctcg
     5401 atgcggcgta ctccgtcgag gtccgccggc cggatcgacc actccgcccc ctcgcagtcg
     5461 cacttcagga agtcgcaccc gccgatcatg ccggcaaact caccgagggt ccgggtcgcc
     5521 acggtgaggg ttctgccccc ccaggtgatc cgctcatcgc ccccgccgcc gagtgccccg
     5581 gagacgaccg tgaccgagac gccgttttcc ctgacgttct cctgcagagc cccggcaagg
     5641 acgggttcga ccgcgagaac acgccgcgac cggcgggcgg cgcggataca gaaagcgccg
     5701 acgttcgccc cgatatcgag aacgatatcc tccggcccga tatcgtcgaa atggtactcg
     5761 ccgacgacgt agacggcgtc ggccccatca ggcatcctcc gaaaggttac gccgtcggaa
     5821 gtgacgaccc gggaggggtc ttttggggca cgctctcggt acacggaata gtattggaat
     5881 ttcggtatac atgggcgttt ctccccgggt gcgcgccacc ccatctgcgg ccccggaggg
     5941 cctgaatcag gccatcttat taatcttttc cggcatattc caatcgtcgc aggagtgcgg
     6001 aatttcattt ccgtccggtg tgcaagacgg tgccggtgtg ccgcgccggt aaaaactcca
     6061 gttatatata aatatattta taaatttaga agtgtaaata atccatgtag cacaaccact
     6121 tgaggggaga tctgctatgg cctacatgga tggaataacc tgtatctgcg gcaacggccg
     6181 gacgctcgag gaccaccggc ccatcgtcgg caacgacgtc atctccggga tctaccgaaa
     6241 agcggcgggc ctccggggaa aacgggtcct ccacgtgaac tcgacctacc agagcggcgg
     6301 cgtggcggag atgatcctct cgctcgtccc gctcatgaac gatgtcggca tcaggacgca
     6361 gtggaacata ctgaacggcg aaggcgactt cttcgtcata acgaagaact tccacaacgc
     6421 cctccagggg caggcgatcg cgttctcgga cgaggagaaa accctctacc tccggacaaa
     6481 cgagacgttc tcacagagaa tggcgatcga ccacgacctc gtggtcatcc acgacccgca
     6541 gcccctgccc ctgatccggt tctacgagaa gacccagccc tgggtctggc ggtgccacgt
     6601 cgacctctcg aaccccgacc ccgccctctg ggagttcctc aagggcttcg ccctcgatta
     6661 cgaccggatg gtcatatcgc acgagcagta ccggcgctcc ggcatgccta tggagcagtg
     6721 gatctgccgg ccggcgatcg acccgctctc ggagaagaac cgcgacctct ccgacgccga
     6781 gatcgccggc ctccttgcga agtacggggt gccgaccgac aaacctctgg tcacccagat
     6841 atcgcggttc gacaagtgga aggaccccga aggcgtcgtc gacgtcttct gcgaggtcag
     6901 gcggcacgtc gactgcagac tggtcctctg cgggagcatg gcccccgacg accccgaggg
     6961 ccgggcgatc taccggcggg tcgaggaggc ggcgcggccc ctcatcgatg ccggcgacgt
     7021 cgtcctgatc accgccgagg accacctgct ggtcaacgcc ctccagaggg catcggccgt
     7081 catcctccag aagtcgctcc gcgaggggtt cggcctgacc gtcaccgaag ggctctggaa
     7141 agggcggccg gtcatcgcct cccgcgtcgg cggcataccg ctccagatag aggacggcga
     7201 gaccgggttc ctcctcgacc cgaccgatac ggaggggttc gcctggcgga tccggcaggt
     7261 ccttgagaac ccggaactcg gcgagaggct cggccgggcc ggcaaagagc acgtccggca
     7321 gcatttcctg atcacccgcc tcctctccga ctacctggat atgctcaacg ccgagctcat
     7381 cggccggaac gcctgaagaa taaagcgata cgcgagatcc ggagggaccg gtttccccgg
     7441 aatcggtctc tccggaaagg tgcgcggcgg ggtgccccgc cgcgtccggg atgcaggata
     7501 ggcggcgcag gtgccggctc aacatggtca gcggtgctgg atgccgggta ttcttttcta
     7561 tcaatatcct gttgtgcaca tgcggaacac agggcaggta ctatggaagt ttttgatggc
     7621 gatgagactc cggaagaatt cagatatcac gtccaaccgg ttgacgcaac caccccccaa
     7681 tgatgacagg atatacatcc ggacatataa ttgtttctca cgccggcgaa cggtgccggt
     7741 agccatgggg tctccttcac gctcccggtt gtcgtgaggt acccccggcg gcgctcgccg
     7801 ggtgcgccgg ggtttctcca aaaaaggttc cgggagtgcg aaagatactc gtctgcggca
     7861 ccgcccgccg gccggaaaag gatgaaaccc ggggccaccc tcaccggccg gcggtctcga
     7921 tcgggccggg cagcctgata ccggcgatgg tctttccctt catcccctcg atgacctggt
     7981 cgacggcacc ctggggcacc tcgacggtgg agtaagtctc gtatatgttg atcgcgccga
     8041 tggcgccgcc gggtatgccg gtctcgcccg cgagcgcccc gacgatatcc ttcggccgga
     8101 cctggtgctc tcttccaagc ggtatccgga ggagtgcgag cccggtctcc tgcgccggcc
     8161 cctggccgcc cgcaccgagc tccagtttca ggagggcggc ggcgacttca agcgacgtgt
     8221 aatcctcaga cgttattcgt tcgaccaggt tcacgtagcg ctcgaggctg ccctcgtcga
     8281 tgacctgctg cacacggtcg atcagttttc gcgtccggct ctcctcgacg tcccccggcg
     8341 tcgggagcgg gatgcgtgcg atctggatcc gggtgtagtt ctggatcgtc cggagcttga
     8401 agtactcttt tgggcccacg aacgtgacgg cccgcccggc ccgcccggcg cgtgccgtcc
     8461 ggccgatgcg gtggatgtag tactcgatgt cctgcgggac gtcgtagttg atgaccaggt
     8521 cgacgtcctc gacgtcgatg ccccgggcgg cgacatccgt cgcgacgagg atgtcgatgg
     8581 acccggcgcg gaactttgcc atgacccggt cccgcagggt ctgtttcata tccccgtgga
     8641 gaccttcggc aaagtagccc cgggcctgga ggtgggtggt caggtcgtcg acgccccgct
     8701 tggtgttcgt gaatatgagc gtcaggtccg ggtcgtacat gtcgagcagg cgcgagagga
     8761 tctcgagttt gtcccggctg cgcacctcaa ggtagagctg ctcgatctgc gggacggtca
     8821 cctcgcggcg cgcgaccgat atgaactcgg ggtccttctg gaacctcttt gatatctcta
     8881 agatgggttt agggagggtg gccgagaaga ggaccgtctg gcggccttcc ggtgtctcgt
     8941 cgagtatctt ctcgatgtcc tcccggaaac ccatatccag catctggtcc gcttcgtcaa
     9001 ggacgacgag gttcaccgcg gcaaacgaga gggtcccgcg gtcgaggtgg tcgagcaccc
     9061 gccccggtgt cccgacgacg atctgggcgc cgcgctgcag ccccttgaac tgccgctcga
     9121 taggctggcc gccgtatatc gggagtatgt tgatgccccg gtggtgcttt gccaggcggg
     9181 cgaactcctc ggcgatctgg atggcgagct cccgcgtggg ggagaggacg agaacctggg
     9241 tctcccggct ttcggggtcg atccgctcga tcgccgggat gccgaacgcc gcggtcttcc
     9301 cggtcccggt ctgggcctgg ccggtcacgt cgcgcccttc gagtatcgcg ggtatcgtgc
     9361 tcacctggat gggcgtgggc tcctcaaaac ccagatcctc tatcgcccgg agcatctttt
     9421 gcgatatatt gaactccttg aatgtgatat tgttctccat tcatcacttc ttggtgttgc
     9481 tacctcttta tccctgcggg acaccggtgg cccgagagcg gtgatctcta tccggtcggc
     9541 aaagagacta ccagtatggc tgcacgatac cccccgcccg tgagcgccgc ggatatgcgc
     9601 gaggtctacc gccggtatgc ggccgaccgc ccgggtctcc gcggcatcct cctccccgac
     9661 ctcctgccgc acgacgcctg cggcgcaagc ccggctcccg gccgcatacg ggtgctcttt
     9721 gtcgccgagc ccccgccctg ggccgccggg ccttccgact gcctgagccc cgactacccc
     9781 tactactatg acgaccgcta cgacgccggg cggaaacgcc gctctccggc agtcttgcgg
     9841 agaacctctt tactctgctc ggcctcgacg gcgggacgcg ccgcgagaac ctggaggcct
     9901 tcgccgggaa gggatgctac ctcgtcgaca cggtccggtg cgtcttccga aagaaccgaa
     9961 aagcggccat accggccgac ctcatccggg tgagcgcgag ggatatcctc gcccgcgagt
    10021 tcaaggcgct ctccccggat tacgtcgtcg ccaaacacgg cgcttgccgg tctccgggag
    10081 atcgagcccc gcgcgagcgc tcttcgtgat gccgagaaga tcaccgcgat cggtgccgac
    10141 cgccgggagc accgtctcct ctgcgtaccc taccccggcg ggcggaaccg gcgctacctg
    10201 ggcgcgatcg agtcgggatt tcgggccatc ggggatcttc tgccctgatc cgcctatccg
    10261 gcaagcttct tctttccctc tgcgtagagt tcctctttgc gcttgcggag ccgtgcagcc
    10321 gtcgatcgct cttcagggct ccccggctct tccagctgga tatccctggc ctctttcgcg
    10381 atctcccggg gatccaggta cccggacggc tccaccttct cccggtcctc ccggtagagt
    10441 ttttctttct gctgcctctg ctcctctgca gcggacaggg cagccttccc ggaggttcct
    10501 cccacttcga tctctctccc gcgggcctcg gcgatcttct tcaaccgcac ctcgaggaca
    10561 ccgttccgga aggtggccga tccctctccg gtgacgtcgg cgggcaggga gaccgtccgg
    10621 gcgatcgcac cgacccttcg ctcccgcacg tagtacccgg tctcctcccc ctcgtgtatc
    10681 atctcccggc gtgcggttat ccgcaacgtc ctcgggtcgg tgagccgaac cgatatgtcc
    10741 tggcgctcga cgccggggag gtcggcgacg accagaacgt ccgtctcgtc ctcccggacg
    10801 tcgaccgtcg tccggatgcc gccgggaagc gccacctgct gcgcggctcc cgatatgcgt
    10861 cccatcgtct cctcaaactg cctctgcatg ttcgcgatca tatcgtcgaa ctcgccccag
    10921 ggttcccggg ccattcttct ccatgccatg gattactctc tcctgcctct cggcgccctc
    10981 gggttgtgaa cacatgatat atacattcct gtgcgggggg tgccgccgca ccgtcctgcc
    11041 gtgcgcggca agagaggtca gccggtgccc cgccctcacc ggatctcagc aataccaact
    11101 gttttcccgc gaccgcacaa acaatgagtc actcgtgatg ccgcatgttc cgcttcaacc
    11161 tcagcccgtc gctcatcggc cggttcttct accacgactg cgagcgctac ctccgttacc
    11221 acgcgacgcc ggagcaggag cgcgagaacg ccggcatccc ggcgattgcg gtcgacacga
    11281 gcccgacgac ccgggcgctc ctcgatgccg gcatacggtg ggaagaagag gtggtccgga
    11341 cgaggctttc gggacgggtg cgggtcccgg gcgggacggg gccgatatct gagcgatcgt
    11401 tctccatcga ggagagtttc ggcctcatcc cgagcctctc cccgggcgac gcaatctacc
    11461 agacgaccat accggtctcg gtccatttcc tgaagggcta cggcctcgac ccggatatgc
    11521 accggttctc cccctgccgc cccgacctca tcagggtcga cgaagaaggg cgcctctctg
    11581 tcatcgacat caaggcaagc gaagacctca gcgtcagcca ccgggtccag gcgaccctgt
    11641 atgccctgat actcgaccac gccctcgacc tgctcggtat cagcgtcccg gtcgacctct
    11701 cccgggcggg gatctggctc tacggcaagg acgaaccgga gtacttcgac ctctccctca
    11761 acatacgggt gatcgaggac ttcttccgcc accgcctccc cggcatcctt gccggcccgc
    11821 cggaggacgt gccctggcac ataacgccgc ggtgcgaagc ctgcgagttt taccctcact
    11881 gccgcgccgg ggcagaggcc tcttcatcgg tatcgctgat cccgggcctc tcgcccacag
    11941 cacggcggta cctccgggag gccccgtggg acggcgggtt ctcgatcaac accctcgccg
    12001 acctcgccgc gttcctccgc gacccggcaa gcgaccgcca cctcgacgac tgcgggtcgc
    12061 ttgcgggccg gggcgaccgc ctgcgggcaa ccgtccgggc cctcgccacg ggagaggtcg
    12121 tccggctcgc cgggacgtcg cttgccctcc ccgtctacga ggatatcgcc atcatcctct
    12181 cgctccagaa ggaccccgtc tccggcgggg tctacgccct cgggttccgg cggagcaggg
    12241 gcgaggcggt ctacgggacg ccctcgcacg aggcgatcta tgtcgcggcg gacgagggcg
    12301 actgcgcccg ggtgcggcag gagttcatcc gggcgctcgc cgccgaactc gcggccgtcc
    12361 acgactacaa ccagggccgg gagtgggcgg accagaagtc ggtgcagacc tacgtctacg
    12421 acgctaccga gggggaactc ttcgcccggt cgatccggga ggggctctct gatcccgcga
    12481 cggcggagga tgcgctcagg ctgcagttca cctaccagga gaccgatatc gcccgggggt
    12541 ccagccaccc ggcaggctcg gtagcgttcc cgctcgtcgt catcacgggg gagatacggc
    12601 ggatgctcgc tctgcccgcc ccctttgctc tccgcctgcc cgaggtcctt gcgatcattc
    12661 cctcctcccg gtttgcgtat accctcagcg caagcagcct cttctggaac gaactgagca
    12721 acgccatgaa gggcgacgcg gtcatcatgg cgtggagcgg gcgggcggag gccgcgggct
    12781 ggatccggca ggaggtctcg cgccggctgc ttgcggcggg gagcatcctt gacggtcttc
    12841 gcgagcgggt gaaggccggc ctcgtgcggt ggccggagaa cttccggttc ccgcgcccgt
    12901 gggatgccgc gacgccggag ctatcgcgcc tcctcttcgt gacggagtac gagtcgacgc
    12961 ttgccgcccg gcggatgcag gaactgcgga gcaggccgtg gcccgtgcgg gtccgggagg
    13021 ggatcagcat accgctcgag aagagcgagg gaaacttctg gaaggtgcgt acccccctcg
    13081 atctcgctct cttcgaacag tcgcaggcgt tcgggcacct cctcgtgccg gacggcgatg
    13141 cgggggagga ggcgcagctc gggtttgacg acctccgcta ccgggatagc ccgaaccccg
    13201 ggaagagcgg tgtctccttt gcccgggtcc acgacagcat cgtcgaccgg gaggcgggca
    13261 tcgtccgggg gctcgtcctc acggtgaacg tcggccggga ccgcacgccc tttgtcgagg
    13321 gcgaccgtgc ggtgctgcat ccccggttca ccgacttcac cgcgccgcgt tacgtcgacc
    13381 gcctccttgc cctggaccgc cagccggaga acacgttcgt ccggctcctc cgcgacccgc
    13441 gggggtttgc cgccccggtc gcggagacgg aggcggtcat agcggacgcg gagcagaccg
    13501 tcctcgacgc cgggttcacg aagagccagg cccgggcgtt tcaccgggtg atgacgggcc
    13561 ggctcacgct ggtatggggg ccgccgggga ccgggaagac gcattttctc gcgacggcga
    13621 tactcgccct cgtcagggcc cggcgggcgc acggggagcg gatccgggtg ggggttacgg
    13681 cgttcaccca cgccgccatc gagaacctgc tcgtccgggt gcaggactcg gtcgacgaat
    13741 tcgggcttgc cgcggggttc ccgatataca agctgaagga cgcccggacg acccggggcg
    13801 agcggagcct cgaggttctg cctcacgacc gggccgacac cgtcgtcggc tacccggccc
    13861 tcctcctcgg ggggacggtg cacagtttcg agcggctcga gaagtccctt ccgtctcttg
    13921 acctcctcat cgtcgacgaa gcgtcccaga tgaggccggc agacctcgcg atggtcctcc
    13981 ctctgctcgc cgacggcgga aggctcgtcc tcgcgggcga cgacctgcag cttcccccgg
    14041 tcatccaggg ggagtacccg gagccggagg acggtctccc gggactctgg gactcgatat
    14101 ttgcctacct ccggcaccgc gacgatcctg gccgctacac ctgccagctc caggagaact
    14161 ggcggatgaa ccggaccctc tcgcggttcc cggcggagac gctctacggc gccggctacg
    14221 ctcccgcgaa cgatgcggtc ggggagcagc ggatcgccct tgccccgggc ttgccgggcg
    14281 aggaatggat cgcatgggcg ctcgatccgg catacccgct cgtcctctgc atcctcgaga
    14341 acgtccggac gacggtcgag aaccaggtcg aggcggggct cgttgcacgt cttgcctgca
    14401 cgctccggga gaggcttctc gatccggcct cggggaaccc gtacccggcg acggcggacg
    14461 gcgacgccct cttctggcgg cgcgggctct tcgtcgtcag cccgcaccat gcccagatcg
    14521 gcgccatccg cgactgcctc tcccgggaga gggcctggga gtcccggccg ttcgtggata
    14581 ccgtcgataa gatgcagggc caggaggcgg aggcggtcat cgtcagttac ggcgtaagcg
    14641 acgtcgagac cgctctctcg gaggccgggt tcatctacag caggaaccgc ctgaacgtct
    14701 cgctgacccg gggccggtcg aagtgcgtgg tcttcctgcc ccggccgctg ctcgagccgc
    14761 cgctcgacct tgtcggggac ccgacggcgg cggcggggct ccggcacatg ctcgacctcc
    14821 aggagttctg ccggacccac ggcgaggaga agacgttctc cggggatgcg cgaggggtcc
    14881 ggctcacggt gatgtgcgct cgggtggagt gaaccggccc aacctatatc cccgtcccga
    14941 ccgaaacgtt gggtgtgatc ggcagtctcg atcccgacca gaacgagcgc gaacagcgga
    15001 tcctcaaaga aacccgcgag cacggcgcca ccccggaggc cgtccgcctc tttcgcgccc
    15061 tcgtcctcgc gcactaccgc gcccacggcc gcgaccttcc ctggcggcag accaccgacc
    15121 cctaccggat actcgtctcc gagatcatgc tccagcagac gcaggtcgag cgggtcgccg
    15181 tgaagtaccc ggagttcatc gaccggttcc ccgactttgc aagcctcgcc cgggctccgc
    15241 agagcgatat cctccttgcc tggcagggga tgggctacaa ccgccgggcg ctcgccctca
    15301 agaggaccgc agagcgggtg gtcgacgact acggcggccg gctcccggcc gacgtcgaga
    15361 ctctcgcgac cttccccggc atagggaggg cgacagcggc ggccatatgc gcgtatgcct
    15421 tcaacatgcc ggtcgcctac atcgagacga acatccggcg ggtcttcatc cacttcttct
    15481 tccaggaccg cgagggtgtc agggacgacg agatcctggt gctcgtcgag caggcgctcg
    15541 accgcgagaa tccgcgggag tggtacggtg cgttgatgga ttacggctcg atcttaaaga
    15601 agcggacggc aaacccgaac cggcggagcg cgtcctactc ccggcagagc cggttcgagg
    15661 gctccgaccg gcagatcagg ggaaggctgc tcgctctcgt cctcgcggag ggcgccgtca
    15721 cgggggagga ggcggcggcc cgggtcgctg ccgaccccgg ccgggcgagg aggatcctcg
    15781 acgacctcgt gcgggagggg ttcgtcgcgg agagcgaggg gacgtatacg tgccggtgag
    15841 gggcagaccc cgggcggcag aatccggcac ccggcatcga gtaatgtggt cttgcggcgc
    15901 cttaacatgg ggccggcagc acccggacgc cggactttgc ggcaacgcgg ga
//
LOCUS       DAOSEE010000038        15553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1523_length_15553_cov_258.036521, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000038 DAOSEE010000000
VERSION     DAOSEE010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 15553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15553
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1523_length_15553_cov_258.036521"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(243..857)
                     /locus_tag="PLI76_04460"
     CDS             complement(243..857)
                     /locus_tag="PLI76_04460"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HOI61318.1"
                     /translation="MPKKKKTFENVYQFKITLQEIRPPIWRRIQVPETYSIWDFHVAI
                     QDAMGWTDSHLHEFQMKDPKSHAEVEIGFPSEFAPGILLSWETAMADFFAINNPRAVY
                     TYDFGDDWRHMVILEKILPKEPGIRYPRCIKGKRACPPEDIGGVWGYEEYLEKLRNPD
                     ARDEDDEYCDFDFDPEHFSCAEVVFDDPEERLKQSGILDSLCID"
     gene            1184..2806
                     /locus_tag="PLI76_04465"
     CDS             1184..2806
                     /locus_tag="PLI76_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61319.1"
                     /translation="MSSCFSDPHILEFFAIGFDLMGGHLPPSAVAPIITDAEDVLEKY
                     PDNRGAALALDALNDLIGERDEALIALESLAMLSESSINMLRRARQLMLSGKTEEAEG
                     LLLEITRMRTAGSVPRELHIMLAFARLGDREAFARIWHDLIERDGLLEPVPAEEFIKY
                     PGDYTLLGEFPFREQIESLEYLFSYGVQENREAEVFAFIHSLPDYLENLLTRVDLEER
                     EYGISGCFAALPVIKAIAEGSLDVIEKIFSTYDPISDERLLEETGENIERIRKSGVEA
                     GVLNELLRWSVDPVQPAGKLLDTLRRHTGGDDEPILAMFDMANMGASGVAVGELASLL
                     LEAHPDNRCLIESVYGSLKSEERYDEALAFADRHEFLKQDDAQFESLKLEALRSSDEE
                     AAFQFLLGGIKERRMLERYADLFELALALDRMDEVASLRWIFAEEKGAIAGSHMLNAL
                     DRLKKRDVKEGTALFERAKRSGFPEELALMLLARSLLSAGYPKRVVGLREKMLKTSLP
                     PEEIYPLLIRAYRELGRESEARDVEAMLEAVR"
     gene            complement(2931..4523)
                     /locus_tag="PLI76_04470"
     CDS             complement(2931..4523)
                     /locus_tag="PLI76_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOI61320.1"
                     /translation="MLLLLFSVMLPASAGLYTDSCGMVWTDAALPFERNNETAYTEEL
                     MASYGPIMANLTEAPDLSNMTWSDAFREACACMEERYAFTEWRGVDWDALYATYAQKI
                     ADAERSKDTAAYYRTLREFIFAIPDGHVLVLSPDDFGAKHADIGGGYGFAVARLDSGE
                     VIVTYVASGSEAERAGIRFGDEVVSWNGRPIGEAINATPILWTPIKPSTAEGILLQKQ
                     RFLARAPVGTTTEIGIAGPTPRTVSLTATDDDYETLARTSIFLGRETNDGAAGSSAEL
                     KAMLTNETVTTRTLPGGIAYIAVYEESYDVYQPFRAAMETAIAGGAPGIVIDLRFNRG
                     GDDNLAAAFAGWFVDRPVFYEYGTTYDPGTGEQAVLWESWSEPRPDRYEGPVALLVSP
                     YTISSGEGLPKVFAETGAGAIVSWYGTNGAFGMESMGPVLPLGIMTAFPQGASLDELG
                     RIQVDSNRSMIGGVAPTVRVPLDRDTLERAMAGEDVQLSYAVAWIEAQAGGEPAPTPK
                     AAAGWVIPLAAIIGLVVWFGRR"
     gene            complement(4572..5147)
                     /locus_tag="PLI76_04475"
     CDS             complement(4572..5147)
                     /locus_tag="PLI76_04475"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOI61321.1"
                     /translation="MQSDTMLIREAAREDLAAVLSLYAHLHDADEKAGERVLERICLE
                     IPADPRTHLFILEYEGAPVATCVLHIHSNLTRGARPYGLIENVVTHREYRNRGFGTTL
                     PHSRAGLRVGAELLQGDAPDRPERTERLPALREGRISQGCKGRAHRISAGVIHSHIVP
                     ALRQPSGTLTVVGPGKISQKPNPAAGKSNIR"
     gene            5316..6866
                     /locus_tag="PLI76_04480"
     CDS             5316..6866
                     /locus_tag="PLI76_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HOI61322.1"
                     /translation="MPNCTTFLDANAANPAKTALIIPSTGESYTRADLLDMVARAAGG
                     LLALGVERGERVCIYLESSVEYLISYLALWRIGAVAVPTNRVYRESEVLYALRDAGAA
                     AVITDAEGAALVGRIRDRTPELRHVVAVSGNIAGTTPWTDLLRAEPCRRAAHCRFDDL
                     CQIQYTSGTTGRPKGAMLTHGNWIAAMDAERDLLGITADDIYLGIYPMGHVGISWGIS
                     TLRAGGTYVVMERFDLSRYIDLAREYRATIVAGMPPVIHSLLQTPPGTEAALATVRVM
                     ISGGGPLMPGVWKPFHERFGIPVANAYGLSETVVVGTGTAIRPEHYATADEFRSVGTP
                     VGFSEVKIVDADDPAQELGPGEDGEIALRGPGVALGYWQMPEETRAAFLPDGWFLTGD
                     IGHLDADGMLAITDRKKDMIVMSGWKVYPTEVEDALIQHPGIRDAAVFGCPDERRGEV
                     PVAVVVPAEGGITPDDIIAFARERLAGYKVPRRVIIAADIPRVNGWKLLRKRLREEYC
                     GAGGTSRA"
     gene            6930..7517
                     /locus_tag="PLI76_04485"
     CDS             6930..7517
                     /locus_tag="PLI76_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HOI61323.1"
                     /translation="MVKTSSRRSTGIAGLDLVLDGGFPPEAHIIVSGSPLSGLELLAQ
                     QFWRTGNAAGAYLMLDTVPLEGMTDARGMDITALVGAVQGERIVIDSLSTLIADRGID
                     AAIRFIREDVQAAVDEGATIVYLLYAGLHGPFEEARVMRAADVFITLRQQIHGNEIER
                     TLAIEKLRGSNVPQRAIPYHIIARGLELSTTSRVV"
     gene            complement(7514..8416)
                     /locus_tag="PLI76_04490"
     CDS             complement(7514..8416)
                     /locus_tag="PLI76_04490"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HOI61324.1"
                     /translation="MTLTSMCLPTTVVGSYPVVKGSGLMSLVDPLKHAVEVAVADQVA
                     AGIDIISDGQVRGDMIHAFTSHLPGIRGSAVVGKVQPARQPITVTDTKYALSRHPKVK
                     GILTGPSTLAHGLKLETSFYRNRDELILDLAQALAVEAGYLQDAGVRLIQIDEPIFST
                     GAANIAVGREAVNAIAGRLRVPVSLHVCGNLGQVIDDVLRIHVAVFDLEFANNPANLD
                     LLSKKDLAGRMIGYGCVDSASPTVESVEIIKKRIEAGIDVLSPSAMLIDPDCGLRMRT
                     RDEASGKLKNMVVAARQVRAEYTD"
     gene            complement(8484..9773)
                     /gene="aspS"
                     /locus_tag="PLI76_04495"
     CDS             complement(8484..9773)
                     /gene="aspS"
                     /locus_tag="PLI76_04495"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050560 - aspartate-tRNA(Asn) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HOI61325.1"
                     /translation="MRLPIRAVTPKTEFAEIIGWVHEVRDLGGLSFFLIRDRTGIIQV
                     TIPKKKVPAEVLDTARNVSRESVVRVRGRVKAIEKAPGGREIVPEELEIISIAESPLP
                     LDVAEKVPAEMDTRIDSRFLDVRKPRVRAIFFIRSTVTCAAMEFLASRGCINITTPKI
                     VAAATEGGTELFPIAYFEKEAFMNQSPQLYKQMMMAAGFEAVFEIGPIFRAEEHNTIR
                     HLNEATSLDVEVSFADHNDVMDLLEDLIVHVYGCVRETCADQIAELGVDLTVPAKPFP
                     RIPYTEAIDIANRAVEEKLSFGDDLSPAAERAIGDVIGQHYFIVDWPTDIRPYYAMPY
                     PDRPEFCKAFDLMHPRMELSSGAQRIHDHDLLVARIRAKGLSPESFEFYLRTFRYGMP
                     PHAGWGLGIERLVMTMLDLSNIREAVLFPRDRHRLMP"
     gene            complement(9917..10459)
                     /locus_tag="PLI76_04500"
     CDS             complement(9917..10459)
                     /locus_tag="PLI76_04500"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HOI61326.1"
                     /translation="MFVPTKCFFTKGVGIHKDKLASFELALRQAGIEKYNLVYVSSIF
                     PPNCQLVSREEGLKHLPTGSIVYVVMARNETNEPSRLASAAIGHAMPKESDAYGYLSE
                     HHAFGETAEVSGEYAEDLAATMLATTLGIEFDPDRAWQEREQIYKASGRIISTTHTCQ
                     AAEGVMDGRWTTVIAAAVFL"
     gene            11014..11256
                     /locus_tag="PLI76_04505"
     CDS             11014..11256
                     /locus_tag="PLI76_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOI61327.1"
                     /translation="MQQDVKPSAVVDCLGLYCPMPIAMTKDAIEKVGIGEVLLVEADD
                     PAAQEDIRRWANRVGHEIVKFEKDGEVIRFSIRRMK"
     gene            11268..11516
                     /locus_tag="PLI76_04510"
     CDS             11268..11516
                     /locus_tag="PLI76_04510"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOI61328.1"
                     /translation="MYADKVVSCIGICQSPMLSIEEEMKMLGEGQILQVMTDKPDLVE
                     MVRSWAGENRHTIEEEHVTSGVTTLILRKGAAAAAEAT"
     gene            11673..12032
                     /locus_tag="PLI76_04515"
     CDS             11673..12032
                     /locus_tag="PLI76_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HOI61329.1"
                     /translation="MARILYIQTSGIDMPERLYAPFVLAMTARATDMDADIYFMIRGV
                     TVVKKGAAEAIQVGNFPKLSEMRDQAIAAGATIYICEQSTQLLGIPRGDFIPEGKVVG
                     ALTLNDLAVDADAVMTF"
     gene            12046..13200
                     /locus_tag="PLI76_04520"
     CDS             12046..13200
                     /locus_tag="PLI76_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOI61330.1"
                     /translation="MEGRIYLDHAAAMPVHPGVARFARQFPESFAGNPSTIYREGLFA
                     QEALAEAREKVAALIGAGDPASIIFTSGATESNNIAIRGAALRNKKTGKMVVTSTIEH
                     ISVLNPAKDLQKSGFTFTQVPVDRFGVIDLDALGAAVTPETVVTSVHYANNEIGTVQP
                     IREVAEIVHDRGGFLHADATDAAGRIPINVGRDGIDLLTLSGNQLGGPDGVGALYIRP
                     GVRVQPVLVGGGQERGLRPGTENVFGIACMGEAARLAGEEMPEESPRLVAIRDRLIAG
                     ITGIEEAFLTGHPAERLPHHASFRFSRIEGESIQLAMNALGIAVATGSACTSRTLEAS
                     HVLLAIGLAHDEAHGSMVATLGRRNSLDEVPRVVNAARETVKRLRAITPL"
     gene            13211..13621
                     /gene="nifU"
                     /locus_tag="PLI76_04525"
     CDS             13211..13621
                     /gene="nifU"
                     /locus_tag="PLI76_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HOI61331.1"
                     /translation="MADQIGYSQKVMEHFMNPHNVGAIENPDGYGKVGNPVCGDLMEI
                     FIRVRDDVIEDIRFRTFGCGSAIATSSMVTDLAKGKTLDEALKITRDDVATELDGLPP
                     RKMHCSNLAADALHAAIRDYREKQKTGEGAAQHP"
     gene            complement(13609..14511)
                     /gene="cofD"
                     /locus_tag="PLI76_04530"
     CDS             complement(13609..14511)
                     /gene="cofD"
                     /locus_tag="PLI76_04530"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HOI61332.1"
                     /translation="MITFLSGGTGTPKLLRGMQELLDEREIAVIVNTAEDMWLSGNHL
                     SPDIDTVMYLFADILDTSRWWGIRDDTYITHDLLARLGIEEFIAVGDRDRAVHVARGE
                     MLRRGMRLTEATAALCANFDVFSTVLPMTDSEVTTYVTTRRGDMHFQEYWVKHRGDVE
                     IEGVVRRHKKPPVATGEAIRAIDASDAVVIGPSNPVTSISPILECAGIREALKRKPVV
                     AVSPFIGDAPVSGPAAALMRAFGKESSSAGTYSIYRDLVDIFIQDIRDTATIDGALRL
                     DTLMTNRGKSLDLAKNILTLIYGC"
     gene            complement(14585..15493)
                     /locus_tag="PLI76_04535"
     CDS             complement(14585..15493)
                     /locus_tag="PLI76_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HOI61333.1"
                     /translation="MITFLSGGTGTPALIRGARQILYDSEIAVVANTAENLRVSGSHA
                     APDIDTAVFLFAGILDTNRGWGIKGDTYATHNYLPKVAGSEPFAVGDRARAVQIARAA
                     LLREGKTLTEAVQAQCRALNIGATILPATDGDATLLVDTGTECLPLFLYRMSAGTDTE
                     VRELIRAAPELPAVTGEVRAAIEASDAVVIGPANPASSILPILECAGMRRLLRDMFVV
                     AVSPFRGDFTPDPKDAALMHAVGETPTAPAVFRLYGDIVDVFVQDIHDPDVVEGSLRL
                     ETRLLHERQAESLAWDIMAVIRHAVS"
BASE COUNT         2900 a         4811 c         4951 g         2891 t
ORIGIN      
        1 cgacgatgaa cgaacggctg cggcagtact acgggagggt gcagcagcac ttcaagggcg
       61 ggctcccaaa agagatccag cctccggaat accagtaggg ggatatccgg gagaaacccc
      121 cggagaactc tttttgtgcg ggatatatcc cctcattatc gtcccggcaa ctttctgtcg
      181 ctcccgagaa cccctctccc ctgcccgatc gggagcgggg tcgcccccac gattatggca
      241 gatcaatcga tgcagagact atccaggata ccactctgtt tcagcctctc ttcggggtcg
      301 tcaaagacaa cctccgcgca gctgaaatgt tcgggatcga aatcgaagtc gcagtactcg
      361 tcatcctcat cgcgtgcatc cgggtttctg agtttctcca ggtactcctc gtatccccac
      421 acacccccga tgtcttcggg agggcatgcc cgctttccct tgatgcaccg gggatacctg
      481 atgccgggtt ctttcgggag gatcttctca aggatgacca tgtgcctcca gtcgtcgccg
      541 aaatcgtagg tatacaccgc ccggggatta tttatggcga agaagtcggc catggcggtc
      601 tcccagctga gcagtatccc gggagcgaat tcgcttggga acccgatctc cacctcggca
      661 tggctttttg gatccttcat ctggaactcg tgcaggtggc tatcggtcca tcccatcgcg
      721 tcctggattg caacatgaaa gtcccagata ctgtaggtct ccggcacctg aatcctcctc
      781 cagatgggag ggcggatctc ctgaagggtg atcttgaact ggtacacgtt ctcgaatgtt
      841 ttcttcttct ttggcatctc tctcacgctc ctctgcaatt ccgcaggcgc accggacgca
      901 ctccatcccc ggtcgtgcag agatctgttt ctccgaagtg atccttctcc ctaccgccaa
      961 aaaaaggttc ctgccgggat cttctctcct atccaccggg cgagagggag cacctgcagg
     1021 cccgggggcg ggtcggagtc caaccccgca aattccgtaa aaattccccc gctacacctc
     1081 atccccggga gttccggcgc atatgacccg caccgcccgg gcgaaactcc tctcctcagg
     1141 ccatcgcaac aactattgcc tccaggattt cacctctcat gagatgtctt cgtgtttcag
     1201 tgacccgcat atcctggagt tcttcgcaat aggattcgac ctgatggggg ggcatctccc
     1261 tccatctgcg gttgccccaa tcatcacgga tgccgaggat gtgctggaaa aatatcccga
     1321 caaccggggt gccgctcttg cgcttgatgc cctgaacgat ctcatagggg agcgggacga
     1381 ggcgctcatc gcccttgaga gtctggccat gctctctgag agctcgatca acatgctccg
     1441 cagggcgcgg cagctgatgc tctcggggaa gacggaggag gccgaaggcc tgcttttaga
     1501 gatcacccgg atgagaacag cgggatcggt accaagagaa ctccacatca tgctcgcgtt
     1561 cgcccgcctg ggcgataggg aagcgttcgc ccgcatatgg cacgacctga tcgagcggga
     1621 cggcctctta gaacccgtcc ctgctgaaga attcatcaaa tatcccggtg actacacgct
     1681 gcttggagag ttcccgttca gggaacagat cgagtctctt gaatacctct tttcgtacgg
     1741 ggttcaggag aacagagaag ccgaagtatt tgcttttatc catagtctcc cggattacct
     1801 ggagaacctc ctcacccggg tagacctgga ggagcgggaa tacggtatct ccgggtgctt
     1861 cgcagcgttg ccggtgatca aagcaattgc tgaggggagc ctcgatgtta tcgagaagat
     1921 attctcgacg tacgatccga tatccgatga acggctcctt gaagagaccg gagagaacat
     1981 cgaacgtatt cgaaagtcgg gcgtggaggc cggcgtgttg aacgaactgt tgcgctggtc
     2041 cgttgatccc gtgcaaccgg ccgggaagtt actcgataca ttgcggaggc ataccggagg
     2101 ggacgatgag ccgatcctcg ctatgttcga tatggcgaac atgggggcat ccggggttgc
     2161 tgtcggggag ctggcctcat tactcctgga ggctcatcca gacaaccgct gccttataga
     2221 gagtgtatac ggttcgctga agtcggagga gcgatatgat gaggcgctgg cgtttgccga
     2281 ccgccacgaa ttcctgaaac aggatgacgc tcagttcgag agcttgaagc tggaagcact
     2341 tcgctcgtcg gatgaggagg cagcctttca gttcctcctc ggagggataa aggagaggcg
     2401 catgctggag cgctacgccg atctcttcga gctggcgctc gcgcttgacc ggatggatga
     2461 ggtcgcctcg ctcagatgga tctttgcgga agagaagggg gcaatcgcgg gatcgcatat
     2521 gctgaatgcg ctggaccgcc tgaagaagag ggatgtaaag gaaggcacgg cgctgtttga
     2581 gcgggcaaag agatccggtt ttccggaaga gctcgccctg atgctcctgg cgcgttctct
     2641 cctctcggcc ggctatccca aacgggtcgt cgggctccgt gaaaagatgc tcaagacgtc
     2701 gctgcctcca gaggagatct acccgctgct tatccgggca taccgggagc tcggcaggga
     2761 atccgaggca cgcgacgtgg aagcaatgct tgaggcggtg cgctgagcgg gaaccgggga
     2821 tagaaccgcg acggaaggtc tccccggatc attcgggggt gaggtgggta ttcaagggga
     2881 gatccttcgt cccggcggcg actcacccga tgccctgagc cgctctcccc tcaccgccgg
     2941 ccgaaccata ccacgaggcc gatgatcgct gcgagcggaa tcacccaccc ggccgccgcc
     3001 ttcggggtcg gggccggctc gcctcccgcc tgcgcctcga tccacgcgac cgcgtacgag
     3061 agctggacat cctccccggc catcgcccgc tcaagggtat ctcgatcgag cggcacacgg
     3121 accgtcgggg cgacaccgcc gatcatcgat ctgttgctgt cgacctggat ccgaccgagc
     3181 tcatcgagcg atgcaccctg cgggaaggcc gtcatgatac cgagcggcaa caccggtccc
     3241 atcgactcca tcccgaacgc cccgttcgtc ccgtaccacg agacgatcgc gccagccccg
     3301 gtctcggcaa agaccttcgg aagtccctcc cccgaactga tggtgtaggg gctgaccagg
     3361 agggcgaccg gcccttcgta gcggtccggc cggggctcgc tccaggactc ccagaggacc
     3421 gcctgctccc cggtcccggg gtcgtacgtg gtgccgtact cgtagaagac cggccggtcc
     3481 acgaaccacc cggcgaaagc ggcggccagg ttgtcgtccc caccccggtt gaaccggagg
     3541 tcgatgacga taccgggcgc accgccggcg atcgcagtct ccatcgccgc cctgaaaggc
     3601 tggtagacgt cgtaggactc ctcgtagacc gcgatatagg cgatccctcc cgggagggtc
     3661 cgggtggtca cggtctcgtt cgtgagcatc gccttgagtt ccgccgacga cccggccgcc
     3721 ccgtcgtttg tctcgcggcc gaggaagatg ctcgtccggg caagggtctc atagtcgtcg
     3781 tccgttgcgg tcaggctgac cgtgcgggga gtcgggccgg cgatgccgat ctccgtcgtc
     3841 gtcccgaccg gcgctcgggc gaggaaccgc tgcttctgga ggaggattcc ttccgccgtc
     3901 gagggtttga tcggcgtcca caggatcggc gtcgcgttga tcgcctcccc gatcggccga
     3961 ccgttccagg agaccacctc gtcgccgaac cggatgcccg cccgctcggc ctcgctcccg
     4021 ctcgcgacgt aggtcacgat cacctcgccc gaatcaagcc gtgcgacggc aaacccgtag
     4081 cccccgccga tatcggcatg tttcgccccg aagtcgtcag gggagaggac cagaacgtgc
     4141 ccgtcgggga tggcgaagat gaactcgcgg agcgtacggt agtacgcggc ggtatccttg
     4201 ctcctctcgg catccgcgat cttttgcgcg taggtcgcat acagcgcatc ccaatcgacg
     4261 ccgcgccact ccgtgaaggc gtagcgctcc tccatgcagg cgcaggcctc gcggaaagcg
     4321 tcgctccagg tcatgttgct gagatcgggc gcctctgtca ggttcgccat gatgggtccg
     4381 taggacgcca tcagctcttc ggtgtaggcc gtctcgttat tccgctcaaa cgggagggcg
     4441 gcatccgtcc agaccatccc gcacgagtcg gtatagaggc cggcggaggc gggcagcatt
     4501 acggagaaga gcaggagcag cacgatcaat ccggctctcg ctccgcgtga aatccttgaa
     4561 aacacgatgc tctaccttat gttactcttt cctgcagcag gatttggctt ttgtgatatc
     4621 ttccccgggc ctactacggt cagcgtcccg gagggttgcc ggagggcagg cacgatgtgg
     4681 ctatggatca ctccggcgga tatgcgatga gcccttcctt tacacccctg agaaatccgg
     4741 ccttctcgta gagccggaag acgttcggtt cgctccggcc ggtcaggagc atcaccttgt
     4801 agcagttccg ctcccacgcg taacccggcg cgtgagtgag ggagggtcgt cccgaacccc
     4861 cggttccggt actcgcggtg cgtcacgacg ttctcgatga gcccgtaggg gcgcgcgccc
     4921 cgcgtgaggt ttgagtggat gtggaggacg cacgtcgcga ccggcgcccc ctcgtactcg
     4981 aggatgaaga gatgggtccg cgggtcggcg gggatctcca ggcagatcct ctccagcact
     5041 cgctcgccgg ctttctcgtc ggcatcgtgc aggtgcgcat agagggagag gaccgcggcg
     5101 aggtcttccc gggcggcctc gcggatgagc atcgtatcgc tctgcatgca ccatcatccg
     5161 ctcctgcaga gatcatcttt gcgcttttac cggcgaggga gcctccgctg gatgtgcagg
     5221 aattatctcc ggcgaccacc ctgttggaca acttatttac ggcactatga acaaacatat
     5281 acatcaaaga gcacacctga caaacggagc ccctcatgcc gaactgcacc acgtttctcg
     5341 atgccaacgc cgctaacccc gcaaagaccg ctctcatcat cccctcgacc ggagagtcct
     5401 acacccgtgc cgacctcctc gatatggtcg cccgggccgc gggcggcctc ctcgccctcg
     5461 gggtcgagcg cggggagcgc gtctgcatct acctggaaag ttcggtggag tacctcatca
     5521 gttacctcgc cctctggcgg atcggcgccg tcgcggtccc gacgaaccgg gtctaccgcg
     5581 agagcgaggt cctctacgcc ctccgcgacg cgggggccgc agccgtcatc accgacgcgg
     5641 agggtgcggc cctcgtcggg cgtatacgcg accggacgcc ggagctccgg cacgtcgtcg
     5701 ccgtcagcgg caacatcgcc ggcacgacac cgtggaccga cctcctccgt gcagagccct
     5761 gccgtcgcgc cgcccactgc cggttcgacg acctctgcca gatccagtac acgtccggca
     5821 ccaccgggag acccaaaggc gccatgctca cccacggcaa ctggatcgcg gcgatggatg
     5881 ccgagcggga tctcctcgga attaccgcgg acgacatcta cctcggtatc tacccgatgg
     5941 gccacgtcgg gatcagctgg ggcatatcca ccctccgggc aggcgggacc tacgtcgtca
     6001 tggagcggtt cgatctttcc cgctacatcg acctcgcccg tgaataccgg gccacgatcg
     6061 tcgccggcat gccgccggtg atccactccc tcctccagac cccgcccggg accgaggcgg
     6121 cgctcgccac cgtccgggtg atgatcagcg gcggcggccc cctgatgccc ggtgtctgga
     6181 aacccttcca cgagcggttc gggatcccgg tcgccaacgc ctacggcctt tccgagacgg
     6241 tcgtcgtcgg gaccgggacc gcgatccgtc ccgagcacta cgcgaccgcc gacgagttcc
     6301 ggagcgtcgg caccccggtc gggttctcgg aggtgaagat cgtcgatgca gacgatcccg
     6361 ctcaagagct cggtcccggc gaggacgggg agatcgccct ccgggggccg ggggtggcgc
     6421 tcggctactg gcagatgccg gaggagaccc gtgccgcctt cctccccgac ggctggttcc
     6481 tgaccggcga tatcggccac ctcgacgcgg acgggatgct cgccatcacc gacagaaaga
     6541 aggacatgat cgtcatgtcg ggctggaagg tctacccgac cgaggtcgag gatgccctta
     6601 tccagcaccc gggcatccgt gatgcggcgg tcttcgggtg ccccgacgag cgccggggcg
     6661 aggttccggt agccgtggtg gtccctgcgg agggcggcat cacccccgac gatatcatcg
     6721 ccttcgcccg cgagcgcctc gccggctaca aagtcccccg ccgggtcatc atcgccgccg
     6781 atatcccccg ggtgaacgga tggaagctcc tccggaagcg cctcagggag gagtactgcg
     6841 gtgccggcgg caccagccgt gcctgacggt tactattaag aaggaaaagc ggtgtataga
     6901 tactcctgac gtacacccga ggtgcagatg tggtcaagac cagcagcaga cgttcgacag
     6961 gtatcgcggg cctggacctt gtgctcgatg gaggattccc cccggaagcc catatcatcg
     7021 tatccggatc ccctttaagc ggccttgagc tcctggcaca gcagttctgg cggacgggga
     7081 acgcggcggg tgcgtacctg atgctcgata ccgtgccgct cgaaggcatg accgacgcac
     7141 ggggcatgga tattacggcg ctggtcggag ccgtgcaggg agaacggatc gtcatcgact
     7201 ccctctccac gctgatcgcc gaccggggca tcgatgcagc catccggttt atccgcgaag
     7261 atgttcaggc ggccgtcgat gagggagcga ccatcgttta cctcctctac gccggcctcc
     7321 acggcccctt cgaggaggca cgggtgatgc gtgcagcgga cgtcttcatc accctcaggc
     7381 agcagatcca cggcaacgag atcgagagga cgctcgccat cgagaagctc agaggctcga
     7441 acgtgccgca acgggcgatc ccctatcaca tcatcgcccg tggccttgaa ctctcgacga
     7501 cgagcagggt ggtctagtcc gtatactcgg cccggacctg tctcgccgca acgaccatgt
     7561 tcttcagttt tccagacgcc tcgtcgcgtg tccgcatccg caggccgcaa tcgggatcga
     7621 tgagcatcgc gctcggcgag agtacgtcga tcccggcctc gatcctcttt ttgatgatct
     7681 cgacgctctc gacggtgggg cttgcagagt ccacgcagcc gtaaccgatc atccggccgg
     7741 cgagatcctt cttcgagagc agatcgaggt tggccgggtt gtttgcaaac tcgagatcga
     7801 agacggcgac gtgtatcctg aggacgtcgt caatgacctg cccgaggttc ccgcagacgt
     7861 ggagagagac cggcacccgg agcctgccgg cgatcgcgtt caccgcctcg cggccgacgg
     7921 cgatgtttgc cgcccccgtg gagaagatgg gctcgtcgat ctggatgaga cgaacgccgg
     7981 cgtcctggag gtagcccgcc tcgaccgcga gagcctgcgc gaggtcgagg atcagctcgt
     8041 ccctgttccg gtagaacgac gtctccagtt tcaggccgtg cgcgagcgtg ctcgggccgg
     8101 tcaggatccc cttcaccttc gggtgccggg atagggcgta cttcgtatcc gtgacggtga
     8161 tgggttgtcg ggccggctgg acctttccga cgaccgcaga gccccgtatg ccggggaggt
     8221 gagaggtgaa ggcgtggatc atgtcgccgc ggacctggcc gtcggatatg atgtcgatcc
     8281 cggcggcgac ctgatcggcg accgctacct ccaccgcatg cttcagcggg tccacaaggc
     8341 tcataagccc ggagcctttc accaccgggt agctcccgac caccgtggtc gggaggcaca
     8401 tgctcgtcag ggtcataaga tcacctgttc cggtcatggg gaagatgcac catttaccct
     8461 tgcgtcaccc gtgcagttaa tcatcacggc atcaaccggt gccggtcgcg cgggaaaagg
     8521 accgcctcgc ggatgttaga gaggtcgagc atcgtcatca ccaggcgctc gatgccgagc
     8581 ccccatccgg cgtgcggggg cataccgtag cgaaacgtcc tcagatagaa ctcgaaactc
     8641 tcgggagaga ggccttttgc acggatccgc gcgacgagaa ggtcgtgatc gtggatacgc
     8701 tgggctcccg atgagagttc catacggggg tgcatcaggt cgaacgcctt gcagaactcc
     8761 ggccgatcgg ggtaaggcat cgcatagtag ggcctgatgt cggtcggcca gtcgacgatg
     8821 aaatagtgct gcccgatcac gtcgccgatc gctctctcgg ccgcgggaga gaggtcgtcg
     8881 ccgaaggaga gcttctcttc gacggcccgg ttcgcgatat cgatcgcttc cgtatagggt
     8941 atccgcggga acggttttgc cgggacggta aggtcgaccc cgagctccgc gatttgatcg
     9001 gcgcaggtct ccctgacaca accgtagacg tggacgatca gatcttcgag gaggtccatg
     9061 acgtcgttgt ggtcggcaaa cgagacctcc acgtcaagcg acgtcgcctc gttgagatgc
     9121 cggatggtgt tgtgctcctc ggcgcggaag atggggccga tctcaaagac cgcctcgaac
     9181 cctgcagcca tcatcatctg cttgtagagc tgcggactct ggttcatgaa cgcctctttc
     9241 tcgaagtaag cgatcgggaa gagctcggtg ccgccttcgg tcgccgccgc gacgatcttc
     9301 ggggtggtga tgttgatgca gccgcgggag gcaagaaact ccatcgcagc gcaggtcacc
     9361 gtcgagcgga tgaagaatat ggcgcggacg cgaggtttcc tcacgtccag gaaccgcgag
     9421 tcgatccggg tatccatctc ggcgggcacc ttctccgcga cgtcgagcgg gagggggctt
     9481 tcggcgatgc tgatgatctc gagttcctcg gggacgatct cgcgcccgcc cggggccttc
     9541 tcgatcgctt tgaccctgcc ccggaccctg accacggact cgcgcgagac gttcctcgcg
     9601 gtatcgagaa cctctgccgg aaccttcttc ttcgggatgg tcacctggat gatgccggtc
     9661 cggtcgcgga tcaggaagaa cgagagccct ccgaggtcgc ggacctcgtg cacccagccg
     9721 atgatctcgg cgaattcggt cttgggggtt acggcccgta ttggtaaacg catgtgaaaa
     9781 acctgggtac aatgtgggcc cggggaggta ttctgtctta tgctcccgac ctggagttgt
     9841 tccggcaggg cggcgggatt gtggcctgca ccggggggag cggggaaaaa aggaagctgc
     9901 cccgggaggt gcggagctac aggaagactg cggcggcgat gaccgtcgtc cagcgaccat
     9961 ccataacccc ttccgccgcc tggcaggtgt gggtcgtgct gatgatccgg ccgctggcct
    10021 tgtagatctg ttcgcgctcc tgccatgccc ggtcgggatc gaactcgatc ccgagcgtcg
    10081 tcgcaagcat ggttgccgca aggtcttcgg cgtactcgcc cgagacctcc gccgtctctc
    10141 cgaaggcatg gtgctcggag aggtagccgt aggcatcgct ctcctttggc atcgcatggc
    10201 cgatagcagc ggaggcgagc ctgctcggct cattggtctc attccgggcc ataacaacgt
    10261 agacgatgct gcccgtgggg aggtgcttta acccttcctc ccttgagacc agctggcagt
    10321 tcggggggaa tatactcgag acgtagacga ggttgtactt ctcgatgccc gcctgacgga
    10381 gagccagctc gaacgatgcc agtttgtctt tgtggatacc cacacccttc gtgaagaaac
    10441 acttggtcgg aacgaacatt aaggatcttc ttatcgtttg aattttttaa gattttcggt
    10501 tataacaagc acaaattgaa aacggaaggg gatatcctct attttttgca ttaggccttt
    10561 aatatatcgc caaatcataa aaacggctaa aaattatcga ttttaaagga tcgtcggagt
    10621 gaccacgacg gctcttctgt ctaccccctt tcacctgcag aatgacgaaa acctgaatcg
    10681 ccgccctatc tccgtgtatc ggggtgcccg gaaagatcgg ggatgagggg agttgagcgc
    10741 atcgggtaag cgatcccggg gagggagcgc caacccggga gaccccgctg cctgcccggg
    10801 gtaccgatct ctcaggagtg cggcctctgc atccgggccc tgcgtatggt cctcctgatc
    10861 ctccggaggg gtcaccggga ctttccggcg ctgctcccca cacagccctc ctcccggagg
    10921 aacgcttata aaggatagtg catgatccgc tgcaaccggg catgcgccgg gagagtgtcg
    10981 cggcacccgg gttccacaac gggggatgga gagatgcaac aggatgtcaa accctctgcg
    11041 gtcgtagatt gtcttgggct ctactgtccc atgccgatcg caatgacgaa agatgcgatc
    11101 gagaaggtcg gcatcggcga agtgctcctg gtcgaggcgg acgatccggc agcacaggag
    11161 gatatccggc gctgggcaaa ccgggtgggg cacgagatcg tgaagttcga gaaggatggc
    11221 gaagtcatac ggttctccat ccggaggatg aagtgaggtg gtgttgagtg tacgcggata
    11281 aggttgtcag ttgcatcggc atatgccagt cgcccatgct gtcgatcgaa gaggagatga
    11341 agatgctcgg cgaggggcag atcctgcagg taatgaccga taaaccggac ctcgtcgaga
    11401 tggtcaggtc ctgggcaggt gagaatcggc acacgatcga ggaggagcac gtcacatcgg
    11461 gcgtgacgac ccttatcttg cgcaaagggg cggcggcagc ggcagaggcg acgtgatccg
    11521 atggatgccg aaggttacgc ggatgtccgg ggtgcctgct gccaggcgcc ggccgtcctg
    11581 ctcggtgagg gggtagatcg gccccaaaga ggaggagccc cgggaatcga tctcgatagc
    11641 cggttttcca tccggaaagg agcgtgaatg gtatggcacg gatactctac atccagacga
    11701 gcggcatcga catgccggaa cggttgtatg cgccgttcgt ccttgcgatg acggcacgcg
    11761 cgacggatat ggatgccgac atctacttca tgatccgggg ggtgacggtc gtgaagaaag
    11821 gcgcggcgga ggcgatccag gtcgggaact tcccgaagct ctcggagatg cgcgaccagg
    11881 cgatcgccgc gggggcaacg atctacatat gcgagcagag cacccagctc ctcggcatcc
    11941 cgcggggcga cttcatcccg gaaggaaaag tcgtcggtgc gctcaccttg aacgaccttg
    12001 cggtcgacgc cgatgcggtc atgacgtttt aaggatgacg gagatatgga aggacggatc
    12061 tatcttgacc acgcggcggc aatgccggtc caccccggtg tcgcccggtt tgcgcggcag
    12121 ttcccggaga gctttgccgg caatccctcc acgatctacc gggaaggact ttttgcacag
    12181 gaagccctgg ctgaggcacg agagaaggtt gcagcgctca tcggcgcggg ggacccggcc
    12241 tcgatcatat tcacgagcgg ggcgaccgaa tcgaacaaca tcgccatacg gggggcagcg
    12301 ctccggaaca aaaagaccgg gaagatggtc gtcacgagca ccatcgagca tatatcggtc
    12361 ctgaatccgg caaaagatct ccagaagagc gggtttacat tcacgcaggt cccggtcgac
    12421 cgcttcggcg tcatcgacct cgacgccctc ggagcggcgg tcacgccgga gaccgtcgtc
    12481 acgtcggtcc actacgcgaa caacgagatc gggacggttc agccgatccg ggaggtggcg
    12541 gagatcgttc acgaccgggg cgggttcctc cacgccgacg ccaccgacgc cgcagggcgg
    12601 ataccgatca acgtcgggcg tgacggcatc gaccttctta ccctctcggg gaatcagctt
    12661 gggggcccgg atggcgtcgg tgcgctctac atccgcccgg gggtcagggt ccagcctgtc
    12721 ctcgtcgggg gcgggcagga acgcggtctc cggcccggga ccgagaacgt cttcggcatc
    12781 gcctgcatgg gggaggcggc ccggcttgcc ggggaggaga tgccggagga gagcccgcgt
    12841 cttgtcgcga tacgggaccg gctgatcgcc ggcatcaccg ggatcgagga agcgttcctc
    12901 accggccacc cggcggagcg gctcccccac cacgcgagtt tccggttctc ccggatcgag
    12961 ggcgagagca tccagcttgc gatgaacgcc ctcggtatcg cggtcgccac cgggtcggcg
    13021 tgcacctccc ggacgctcga ggcctcgcac gtcctccttg ccatcgggct cgcccacgac
    13081 gaggcgcacg ggtcgatggt cgcgacgctc gggcgcagga acagcctcga cgaggtgccc
    13141 cgggtcgtca acgccgcaag agaaacagtt aaacgcctgc gggctattac accgctgtag
    13201 gtgaaggacc ttggcagacc agatcggata cagccagaaa gtgatggagc acttcatgaa
    13261 cccgcacaac gtcggggcga tcgagaaccc cgacggctac ggcaaggtcg gcaaccccgt
    13321 ctgcggggac ctcatggaga tcttcatccg ggtcagggat gacgtcatcg aggacatcag
    13381 gtttcggacg ttcgggtgcg gttcagcgat cgcgacgagc agcatggtga ccgaccttgc
    13441 taaaggaaag acgctcgacg aggcgctgaa gatcacccgc gacgacgtgg cgaccgaact
    13501 cgacggtctg ccgccgagaa agatgcactg ctcaaacctc gcggcggatg cgctccacgc
    13561 ggcgatacgg gactaccggg agaagcagaa gacgggtgaa ggggccgctc agcacccgta
    13621 gatcagcgtc agtatgtttt tcgcgagatc caggctcttc ccccggttgg tcatcagggt
    13681 gtcgaggcgg agcgccccgt cgatcgtggc ggtatcccgg atatcctgga tgaatatatc
    13741 cacaagatcc cggtagatgc tgtaggttcc ggcggacgag gattctttcc cgaatgcccg
    13801 catcagggcc gccgccgggc cgctgaccgg ggcgtcgccg atgaaggggc tgaccgcgac
    13861 gacgggtttc ctcttgagcg cctcacggat gccggcgcac tcgagtatcg gcgatatgct
    13921 cgtgaccgga ttgctcggcc cgatgacgac ggcatcgctc gcgtcgatcg cccggatcgc
    13981 ctcgccggtc gcgaccgggg gctttttgtg ccggcggacc acgccttcaa tctctacgtc
    14041 gccccggtgc ttcacccagt actcctggaa gtgcatgtcg ccccggcgtg tcgtgacata
    14101 agtcgtcacc tcagagtcgg tcatcgggag gacggtcgag aagacgtcga agtttgcgca
    14161 gagggccgcc gtcgcctcgg tgagccgcat ccctctcctg agcatctccc cgcgggcgac
    14221 gtggaccgcc cggtcccggt ccccgacggc gatgaactcc tcgatgccaa gccttgcgag
    14281 gaggtcgtgg gtgatgtaag tatcgtcgcg gatcccccac caccggctgg tgtcgagtat
    14341 atccgcaaag aggtacatga ccgtatcgat gtcgggcgag aggtggtttc ccgagagcca
    14401 catatcttcg gcagtgttga cgatgaccgc gatctcccgt tcgtccagca actcctgcat
    14461 gccgcggagg agcttcgggg tcccggtccc gccggagagg aaggttatca tggagtaatt
    14521 agcgtatcac gcatagtcgt atatgtattt tcccaaatcc tgccgaacag cacctccctt
    14581 catctcacga aaccgcgtga cgtatgaccg ccatgatatc ccaggcgagc gactctgcct
    14641 gccgctcgtg caggaggcgg gtctccaggc ggagcgagcc ttccacgacg tccgggtcgt
    14701 ggatatcctg gacgaagacg tcgacgatat caccatacaa ccgaaaaacc gccggtgcgg
    14761 tgggggtttc gccgacggcg tgcatcaggg cggcatcctt tggatccggc gtgaagtcgc
    14821 cccggaacgg cgagaccgcg acgacgaaca tgtcgcggag gaggcggcgc atgccggcgc
    14881 actcgagtat gggaaggatg ctcgatgccg gatttgccgg cccgatgacg acggcatcgc
    14941 tcgcctcgat cgccgcccgc acctccccgg taaccgcggg cagctcgggt gcggcgcgga
    15001 tgagctcccg cacctcggtg tcggtgccgg cgctcatccg gtagagaaag agtgggaggc
    15061 attcggtccc ggtatcgacg aggagggtgg catcgccatc cgtcgccggg aggatggtcg
    15121 ccccgatatt gagcgcccgg cactgggcct gcactgcttc cgtcagggtc tttccctcac
    15181 ggaggagggc cgcccgggcg atctggacgg cacgggcacg gtcgccgacc gcaaagggtt
    15241 cgctgccggc caccttcggg aggtagttgt gggtggcgta ggtgtcgccc ttgatccccc
    15301 acccgcggtt tgtatccagg atgccggcaa agaggaagac ggcggtatcg atatccgggg
    15361 cggcatggct ccccgagacc cggaggttct cggcggtgtt tgcgaccacg gcgatctcgc
    15421 tgtcgtagag gatctgccgg gcgccccgta tgagcgcggg cgtcccggtt ccgccggaga
    15481 ggaaggtgat cattctccat acctttatca ctcctctccc aagaacctta acggaacgga
    15541 tgaagatcat caa
//
LOCUS       DAOSEE010000039        15394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1539_length_15394_cov_245.541561, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000039 DAOSEE010000000
VERSION     DAOSEE010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 15394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15394
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1539_length_15394_cov_245.541561"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1979)
                     /gene="hypF"
                     /locus_tag="PLI76_04540"
     CDS             complement(<1..1979)
                     /gene="hypF"
                     /locus_tag="PLI76_04540"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HOI61334.1"
                     /translation="MRTQGTIIIRGIVQGVGFRPFVYAQAQKFDITGTVKNLGSEVEI
                     HAFGDRFEEFLGAVSRGPPLSRIDSVEVRDLRGNRPEEFVILESSSGSLSGFIPADVA
                     ICDDCVGDILTPGGRYEGYWATSCVNCGPRYSIITAIPYDRERTAMEAFPMCAACESE
                     YTDPSCRRHHAQTIACAACGPRLSLLRSDGTPVLGDPIREAAALLDAGFIVAVRGIGG
                     FHIACIEEAAGELKRRLGRTEQPLAVMATAGEVERIAVVSDEDRRILRSRERPIVVLA
                     KRDPASHAGISNLHTIGCMLPYTGLHHLLFAHLAHPLLIMTSANLPGYPMITDLAVAL
                     GRLSGEVDYILTHDRRIVNRCDDSVVRDGYIIRLSRGLAPKRAAIDLGDACILGVGPE
                     LNANISVYRNGFCITSPHVGNVRNPETLAYLQETAEKIGGLVGARYDTVAHDLHPQFL
                     STRYAREVAEATGAELLPVQHHRAHIAAVTRDECVGIAIDGVGYGDDGTVWGGEVFAG
                     RVPHLDRVAHLEVVPMPGGDLATRFPERMLYGILPTDSIRDLLSSRGWSDMELAVLAR
                     QVERGLNVAATSSTGRVLDAAAALLGICRERTYDGEPAMKLESAAYAGRPAAWEIEFS
                     RDGGCEVLSTRALLSEAHRRLLAGERVGDIAASVQ"
     gene            2037..2549
                     /locus_tag="PLI76_04545"
     CDS             2037..2549
                     /locus_tag="PLI76_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HOI61335.1"
                     /translation="MTGHIKILNDPVELVPLLITFNNADYKRIYELLNKTWLTEEELG
                     AHADTSTVIACLSILRKGNLVEEQWRMPKPGEKPTKEYRATYSKFRASFQCSMGDIGD
                     LLHIAISNDAGLRSIVDSVEQEIVAGTTSIGDISRKYGVSPIFIRGLAKRIPGLDVKG
                     QGMVLLDRLH"
     gene            2533..3342
                     /locus_tag="PLI76_04550"
     CDS             2533..3342
                     /locus_tag="PLI76_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HOI61336.1"
                     /translation="MTDYTDDPLYVILRSKREATRFQILVEIAEHQPSIRQQEIAGKL
                     GVTPQAVSEYIREMVDEGLVTAHGRGRYEVTKSGIEWVLRHAEALESYARHINRDIIQ
                     QVAVWTAIAREDIRKGDTVGVLMQDGWLYATKQEQNATGRATMDAKPGEDLGVAQLNG
                     LIEHEEGLIHVCKVPRVERGGSRKVSPDLLRAAVRDAEMVAAVGLESYVALRKADIEP
                     DMFFGSREGVIEAAFHGRECAILIVDEEFTDFLKRLEATGLAYTIHDLLAS"
     gene            3339..5348
                     /locus_tag="PLI76_04555"
     CDS             3339..5348
                     /locus_tag="PLI76_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOI61337.1"
                     /translation="MMVIVQPQKGTYKLLFVESGRVEGSGYVDLTATPKGQRPKNFKM
                     RRRGKQLKPTPTRDLITLLRRSSVHLAGRSPEFESFLADLQIHSSRIDICRYCQLEDR
                     FVPVDKATGVRYGGEWICMECAKREMRRELGYMGKFGGSVLVHLEKLLAQVRDLDRVL
                     ASVGPEKPDRSRTLFDRLEAHEVQKTAHITDLALPPAFAKAAGVEYLMPVQQLAVQSG
                     LLEGQHLLVVSATASGKTFIGEMAGMKNFLEGRGRMLFLVPLVALANQKYQRFRDRYG
                     HLVRVTLQTGVSRLNLPETRPAGERDIGAPVVVGTYEGIDHALRTGRPMKGIGTVVID
                     EVQMLEDPDRGHRLDGLIARLKQIAPDAQFLYLSATIGMPGLLAEKLRANLVRYADRP
                     VPLERHLLFIERAKKIGFIKQMVAEEYKIRSSKGYRGQTIVFTNSRARCHVIADTLGK
                     NAAPYHAGLTAQERRDVERRFANGEVAAVVTTAALAAGVDFPASQVIFDALAMGIQWL
                     TVQEFHQMMGRAGRPDFHDLGRVVILAEPGGSYSRTSGKTEEEVAVGLLRGEMEEVAP
                     EYDMEQSSEEFVANAVVCGGDEQQIIRMTRSMVGTLEPALPTLLDYGLVRRRAGRIEL
                     TDMARVMAEHFIGAERLIEIKDLVRRMDDAAEIVAELECAEEKTP"
     gene            complement(5397..6590)
                     /locus_tag="PLI76_04560"
     CDS             complement(5397..6590)
                     /locus_tag="PLI76_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI61338.1"
                     /translation="MNTARRIYNVLFISVFATMLGLGIVSPLLPIYAENLGATGIWLG
                     VIFSAFALSRSVFMPIIGRISDRRGRKWIILTGMFAYAVLSLAYLIAGSVYSLTAVRF
                     AHGLASAMVVPIAMAYIADLSEKGKEGSHMGNFSISMFLGMGVGPLLGGFLNDTFGMP
                     SVFYAMAGLSAFATLLVAISLPEAKPGGFALPDAPSTPMPALITLPVMQGVMVFAFIS
                     ALGRGGMMVFVPVFGPLIAISPFEVGIVLSANTFLMALLQVPIGRLTDTGNKVALIVA
                     GSTIAAIALAVIPLTHSFLPLLLVTSLIGVGGAIQQPAIMALTVDAGRTIGMGTSMGA
                     YNTVFGIGMIIAPLMGGAFMDFVGIEAVFYVGGAISLIGTVIFAAMMQRSARREVAGE
                     TPGPG"
     gene            complement(6700..7539)
                     /locus_tag="PLI76_04565"
     CDS             complement(6700..7539)
                     /locus_tag="PLI76_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61339.1"
                     /translation="MKVADILEVDGCRLVEPSFAELTIDACTALLFQGSPDEARLLVD
                     EEEEPLAFALHDGSNGWHAASFLFRDPTIEVIECFEAVGGDIYQESREAWLSAVREYY
                     SLDIRANVVPALEDLRPDREGNIRDLVTVVWGDRAGTPCLDCCCGSGVGTAALRAGGV
                     RALAYDNDPALLALGLTRGRLAPADTICIDAQEASRYIAPVPLGVAFMAGEIYSYNAD
                     LWRSIIAELLALTDEALITVGTEAEATRVEGWCAGEGWDTRVFENPRDPIYDRWCCVA
                     RRG"
     gene            complement(7536..8384)
                     /locus_tag="PLI76_04570"
     CDS             complement(7536..8384)
                     /locus_tag="PLI76_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOI61340.1"
                     /translation="MEQTGDHDWIKGCTLPDHTELQERTLKTAQDIVIGDRCRIDYGL
                     AGNDVVVCEFSKINGTIVAGGDVRIDNWCEVGGDVIAEEDAYLGEGVKIRGKLVVRGD
                     LDIGDNVQIERGFEAKGWISIRNPMPVIVYIMMYLVAVLGIEKQEDLDAVLQKLFGDD
                     EVRTEAPLMIPAGAVLNMQTFSVPERMAVGSGCRLHGNIRAASIAIREETTIFGSLHA
                     REGAKVARRSVIHGDVQSDGDVVIEQEVHILGNISCRGLTLHEDARVDGVIRAPGGLT
                     IERRTI"
     gene            complement(8401..9636)
                     /locus_tag="PLI76_04575"
     CDS             complement(8401..9636)
                     /locus_tag="PLI76_04575"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOI61341.1"
                     /translation="MIAQKVLFLVLDGISDRPCDVLDGRTPLAAARTPVLDRLAAEGV
                     CGIMDSIAPGIRPGSDTSHLALLGYPPQEYYTGRGPLEAEGTGIRMTAGMIGFRCNFA
                     ATDAEGRVTDRRAGRISGTAPLAEAIRDGVDLSPLGVGFRFESGAGHRAALALAGSGL
                     GDKVSSNDPKRDGVRPPAIRPCTDDPEDRKTAEACNEFIRQSHDILSRHPMNVRRMEE
                     GLAPGNLLLIRGAGKMGAFPQFSSRYGLSGSVISAATLIAGIGKVVGLEHIPVPGTTG
                     SVDSDLDAKVKAALAELDRKDFVLLNIKGADEAGHDGRGIQKRDFIEVIDAALEPLLD
                     LGDTLLVVCADHSTPCSIKDHSADPVPVVIRGPGVRTDRVARFDEVSCAEGGLHRIRG
                     RDLMPIALDLINKSHKYGA"
     gene            complement(9677..10117)
                     /locus_tag="PLI76_04580"
     CDS             complement(9677..10117)
                     /locus_tag="PLI76_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61342.1"
                     /translation="MTGYTSAGGTVTAEEEHTDARQVREMALCPGPPEEQQKMAFGII
                     RRMLVQFHGEWTALREKESDDAWLDAAHALLDRYAHQLHDVVCDVQVIVGDDIVIDIR
                     CLSADMITTTNILVMIGCSEECCRRGDALAKEALRHAERCLSLR"
     gene            complement(10201..10794)
                     /locus_tag="PLI76_04585"
     CDS             complement(10201..10794)
                     /locus_tag="PLI76_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3ae"
                     /protein_id="HOI61343.1"
                     /translation="MARKKQVGRRVEGWKAKKWYRVYVPETFGKVEIGDTISADPANM
                     VGRVMTATLGEVVQDYSKSHIKMRFKINNVAGDAAYTEFVGHEVTRDYLRSMVKRRAS
                     RIDTIHPIVSKDGKLLRVTVVCLTISRAEQSQVHAVRQAISQALSARAAESDFETLVK
                     EIVSGDMARDVFKVVKTIYPVRRVEITKSKLEQIAAV"
     gene            complement(10808..11065)
                     /locus_tag="PLI76_04590"
     CDS             complement(10808..11065)
                     /locus_tag="PLI76_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Pcc1"
                     /protein_id="HOI61344.1"
                     /translation="MTRIEGTIETPHSLPGCVAAALEPDNLTLIRTYPVRGGVRAEIG
                     GTRLRSTIASVDDYLMNLAIAEEICNLPVRSERAAQNKDIE"
     gene            complement(11062..12279)
                     /locus_tag="PLI76_04595"
     CDS             complement(11062..12279)
                     /locus_tag="PLI76_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOI61345.1"
                     /translation="MSLDAAAAGLADHLLEQEFVEVLAHHDADGIAAASILCHAMFRK
                     GGRFRLRIRPGITAADVPEEGSVLLCDFGSTLQDLPGDVMVVDHHLPHFEGDYHVNPR
                     LAGIDGDRNLSAAGTAYIVAQRMGDNRDLAGLALLGILGDGQDIEGPNRDIVNEGIAN
                     GFITPRRGLRLPGRGLVEQLALAVSPYLTGLSGEPDAARALVAGVTDEDDVDIEALLS
                     RVVLTAAPKASHTALCGIWGTTYSLGREVIDEAGNLAAVVDACGKAGCGDIGASLCLR
                     STHALQEAWDVTVRHRRAVVAGIRGASRLDDRLALFTVDDGTVTGDVADALARDFPAT
                     GPVFVIGVSGDGYSVSARCPPDVDLDLDALMRRLAGACGGRGGGHHRRAGAHIGADQV
                     DRFRQGLREAMPA"
     gene            complement(12288..12746)
                     /locus_tag="PLI76_04600"
     CDS             complement(12288..12746)
                     /locus_tag="PLI76_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOI61346.1"
                     /translation="MARMYARRRGTSSSVRPYRKEAPEWSNTDVAEIEKIIVNLRKDG
                     MSSSQIGLALRDRYGVPDVKLATGKRVTEILREKGLDSEIPEDLRNLMQKALGIRKHL
                     SENKKDIHNTRQLQITESKVRRLVKYYVRSGRMPKGWTYKPETAEILLSR"
     gene            13024..15294
                     /locus_tag="PLI76_04605"
     CDS             13024..15294
                     /locus_tag="PLI76_04605"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOI61347.1"
                     /translation="MIESEPDRIADGVRFPQEFLPLFGIAASALVALGVTAYCLISGI
                     FIIFPHLFYIPIILVSYYYPNRGVGATVVLSAAYLAEILFLSPVSEAEIINALVRIGV
                     FLLVAAVVSHLAGRLQVREARYRGIFETSGAGIFLFSPRTGKIEEMNQQCAAMLGYRE
                     GEAPSFDVSTIWPGYTGLTGPLEEGRIEGLDCNLVARDGTPSAVLLSASLLPDQQEGC
                     VVVTGTAELKQMENQLRRSKETLRVILDTTDVGILLTDPGRTIVEANAAAVRFFGGAG
                     REDLLGKDPYDLVAGRDREAWAACRERALHGEAHVFGECTLCRLDGTEWPAEFTITSF
                     AQGGAAPGRLVVSFRDITERRRAEREMREENRRLFVTNEVMAAATASRRLDDLLPAAL
                     EKSLALLDFDLGAAHLVYPGDDRAHLRASVGDVVFPLDVSRDEPPYRYVLVDGEARFV
                     DHFQEKYPEHGNPGTRSFASVPIPGDDGPVGCITVASRARETIPEGERRILVAIGEGL
                     GSAVVKGMLHEDLEAALASANHYLEKANAATEAANLYVDILTHDINNANTVAMGYLHM
                     ALESGDKPPREFLREPLAAIYQSSDIIRNVTTIRRLEAGDVKLRPVRLESVFRGMRNY
                     FADARITCEGPDATVLADDLIDEIFSNLIGNAVKFGGPDAGITISVREEGEAVSVTVA
                     DNGPGIPDDLKPRIFERKVRGTTKKSGKGLGLSIVRMLAERYGGSVRAGDRVPGRPEE
                     GAAIMVTLRRYKAGEE"
BASE COUNT         2739 a         4945 c         5025 g         2685 t
ORIGIN      
        1 tactggaccg acgcggcgat atccccgacc cgctcgccgg ccagcaaccg ccggtgtgcc
       61 tccgaaagga gcgcccgcgt cgagaggacc tcgcaccctc cgtcgcgcga gaactcgatc
      121 tcccaggccg ccggccgccc cgcatacgct gccgactcaa gcttcatcgc cggctccccg
      181 tcgtaggtcc gctcccggca gataccgagg agggccgccg ccgcatccag cacccggccg
      241 gtgctcgagg tcgcggcgac gtttaacccc cgctccacct gccgggcgag gaccgcgagc
      301 tccatatcgc tccagccccg cgacgaaagg aggtcccgga tcgaatccgt cggcaggatc
      361 ccgtacagca tccgctccgg gaaccgggtg gcaagatcgc cgcccggcat cgggacgacc
      421 tcgaggtggg cgacccggtc gaggtgcggg acccgcccgg caaagacctc cccgccccag
      481 accgtcccgt cgtcgccgta gccaacgccg tcgatcgcta tcccgacgca ctcatcacgg
      541 gtcacggcgg ctatgtgcgc ccggtggtgc tggacgggaa gtaactccgc cccggtcgcc
      601 tccgcgacct cccgggcgta gcgggtcgag aggaactgcg ggtggaggtc gtgggcgacc
      661 gtatcgtatc gtgcgccgac gagccccccg atcttctccg ccgtctcctg caggtaggca
      721 agggtctcgg ggttcctgac gttccccacg tggggcgacg tgatgcagaa cccgttacgg
      781 tagaccgata tgttcgcgtt cagttccggg ccgacgccga ggatgcacgc atccccaaga
      841 tcgattgctg cccgcttcgg ggcaaggccc cgcgagagac ggatgatgta gccgtccctg
      901 acgaccgagt cgtcgcaccg gttgacgatc cggcggtcgt gggtgagtat gtagtccacc
      961 tcgcccgaga gccggccgag ggcgaccgcg agatccgtga tcatcgggta gcccggcagg
     1021 ttcgcgctcg tcatgatgag gagcgggtgg gcaaggtgcg caaagaggag gtggtgcaga
     1081 ccggtgtagg ggagcatgca cccgatggtg tgaaggttcg atatacccgc gtgagacgcg
     1141 ggatcccgtt tcgcgagcac cacgatcggc cgctcccgcg agcggagaat ccgccggtcc
     1201 tcgtccgaga cgaccgcgat ccgctccact tcgcccgcgg tcgccatcac cgcgagaggc
     1261 tgctccgtcc ggcccaggcg gcgtttcagt tcgccggccg cctcctcgat gcaggcgatg
     1321 tggaaccccc cgatcccccg gacggcgacg ataaaacccg cgtcgaggag ggctgccgcc
     1381 tcgcggatgg gatcccccaa aaccggcgtc ccgtccgacc ggaggaggga gagacggggg
     1441 ccgcaggcgg cacaggcgat cgtctgggcg tggtggcgcc ggcacgacgg gtcggtgtac
     1501 tcactctcgc aggcggcaca catcgggaac gcctccatgg cggtgcgttc ccggtcgtag
     1561 gggatggcgg tgatgatgct gtagcggggg ccgcagttga cgcaggacgt ggcccagtat
     1621 ccctcgtagc gcccgcccgg cgtgaggata tcgccgacgc agtcgtcgca gatcgcgacg
     1681 tcggccggaa tgaaccccga gaggcttccc gaggaactct cgagtatgac gaactcttcg
     1741 ggccggttgc cgcgcagatc cctgacctcc accgagtcga tgcgggatag cggcggcccg
     1801 cgggagaccg ctcctaaaaa ttcctcgaaa cggtccccga aggcatggat ctccacctcg
     1861 ctcccgaggt tcttgacggt cccggtgata tcgaatttct gcgcctgcgc atagacgaag
     1921 gggcgaaatc cgaccccctg gacgatgccc cgtatgatga tggtgccctg tgtacgcatc
     1981 gaaaattcag caaaaattta tggagttata ccacgatata ataatagggt atcgcattga
     2041 cgggacatat taaaattctt aacgatccgg tggaactggt tcctcttctt ataacgttca
     2101 ataatgcaga ctataaaaga atctacgagc tcttaaacaa gacctggctg accgaagaag
     2161 aactgggggc ccacgccgat acctcgaccg tgatcgcgtg cctctccatc ctcaggaagg
     2221 gcaacctggt cgaggagcag tggcggatgc cgaaacccgg agagaaaccg acgaaggaat
     2281 accgggcgac atacagcaaa ttcagggcca gttttcaatg ttccatgggc gatatcgggg
     2341 accttctgca catcgccata tccaacgatg caggcctccg ctcgatcgta gactcggtcg
     2401 aacaggagat cgtagccggg accacatcga tcggcgatat atcccgaaag tacggggtca
     2461 gcccgatctt catcaggggg cttgcaaaga ggatccccgg tctcgatgtg aaagggcagg
     2521 gaatggtgct gcttgaccga ctacactgac gatccgctct acgtcatcct gcgcagcaag
     2581 cgcgaagcca cccggttcca gatcctcgtg gagatcgccg agcaccagcc atccattcgc
     2641 cagcaggaga tcgccgggaa actcggcgtg acaccgcagg cggtctccga atacatcagg
     2701 gagatggtgg acgaggggct cgtcaccgct cacggccggg ggcggtatga ggtgaccaag
     2761 agcggcatcg agtgggtcct gcggcacgcc gaggcgctcg agtcctatgc ccgccacatc
     2821 aaccgcgaca tcatccagca ggtcgccgtc tggacggcca tcgcccggga ggatatacgg
     2881 aaaggggata cggtcggcgt cctgatgcag gacgggtggc tctatgcgac gaagcaggag
     2941 cagaacgcca cgggcagggc gacgatggat gcaaaacccg gggaggacct cggtgtcgcc
     3001 cagttaaacg gcctcatcga gcacgaggag gggctcatcc acgtctgcaa ggtgccgcgg
     3061 gtggagcggg gcgggtcgcg gaaggtctcg cccgatctcc tccgggccgc cgtccgggat
     3121 gcggagatgg tggccgccgt cggcctcgag tcctacgtgg ccctcaggaa ggcggatatc
     3181 gagcccgata tgttctttgg ctcgcgggaa ggggtcatcg aggcggcgtt ccacggccgg
     3241 gagtgcgcga tactcatcgt cgacgaggag tttaccgact tcctcaaacg gctcgaggcg
     3301 acggggcttg cctacacgat ccacgacctg ctcgcatcat gatggtcatc gtccagcccc
     3361 agaaaggcac gtataagcta ttattcgtcg aaagcgggcg tgtcgaaggc tccgggtacg
     3421 tcgacctgac cgcgacgccg aaaggccagc gccccaagaa cttcaagatg cgccggcggg
     3481 gaaaacagct caagcccacc ccgacccgtg acctcataac gctcctgcgc cggtcttcgg
     3541 tccaccttgc ggggaggagc ccggagttcg agtcgttcct tgccgacctc cagatccatt
     3601 cgtcccggat cgatatctgc cgctactgcc agcttgagga ccggttcgtc cccgtcgata
     3661 aggcgaccgg tgtccggtac ggcggagagt ggatctgcat ggagtgtgca aaacgcgaga
     3721 tgcgccggga actcggctac atgggtaagt tcggcggatc cgtgctcgtc catctcgaga
     3781 aactccttgc ccaggtccgc gacctcgacc gggtgctcgc gtcggtgggg ccggagaagc
     3841 ccgaccggtc gcggaccctc tttgaccggc tcgaggcgca cgaggtccag aagaccgccc
     3901 atatcaccga ccttgccctg ccccccgcgt ttgcaaaggc cgccggcgtc gagtacctga
     3961 tgcccgtcca gcagctcgcc gtccagagcg ggctcctcga ggggcagcac ctcctcgtcg
     4021 tctccgccac cgcgagcggc aagacgttca tcggggagat ggccggcatg aagaacttcc
     4081 tcgaagggcg ggggaggatg ctcttcctgg tcccgctggt cgcgctcgca aaccagaagt
     4141 accagcggtt ccgcgaccgt tacggccacc tggtgcgggt taccctgcag accggggtga
     4201 gccgcctcaa cctccccgag actcgtccgg ccggggagcg ggacatcggc gcccccgtcg
     4261 tggtggggac ctacgagggg atcgatcacg ccctccgcac cgggcggccc atgaaaggta
     4321 tcggaaccgt cgtcatcgac gaggtccaga tgctcgagga ccccgaccgg ggccaccgcc
     4381 tcgacgggct gatcgcccgc ctcaagcaga tcgcccccga tgcccagttc ctctacctct
     4441 cggccacgat cggcatgccg gggctgcttg cggagaaact ccgggcaaac cttgttcgct
     4501 acgcggatag gccggtgccg ctggagcgcc acctcctctt catcgaacgt gcaaagaaga
     4561 tcgggttcat caaacagatg gtcgccgagg agtacaagat ccggtcgtcc aaaggctacc
     4621 gggggcagac gatcgtcttc acgaattcgc gggcccggtg ccacgtcatc gccgataccc
     4681 tcgggaagaa tgccgcgccc taccacgcgg ggctgaccgc ccaggagcgc cgcgacgtgg
     4741 aacggcggtt tgcgaacggc gaggttgcgg ccgtcgtgac cacggcggcg ctcgccgccg
     4801 gcgtcgactt cccggcgtcg caggtgatat tcgacgctct cgcgatgggc atccagtggc
     4861 tgaccgtcca ggagttccac cagatgatgg gccgggccgg ccggcccgac ttccacgacc
     4921 tcggccgggt ggtcatactc gcggagcccg gcgggtcgta ctcccggaca tccggaaaaa
     4981 cagaggaaga ggtcgccgtc ggcctcctcc ggggcgagat ggaggaggtg gcccccgagt
     5041 acgacatgga gcagagttcc gaggagttcg tcgcaaacgc cgtcgtctgc ggcggcgacg
     5101 agcagcagat catccgcatg acccgttcga tggtcgggac gctggagccg gcgctcccca
     5161 ccctcctcga ttacgggctg gtccggcggc gggcaggacg catcgagctc accgatatgg
     5221 cccgggtgat ggccgagcac ttcatcggcg ccgaacgcct gatcgagatc aaggatctgg
     5281 tccggcggat ggacgacgcg gccgagatcg tcgcggaact cgagtgtgcc gaggagaaga
     5341 cgccgtaaaa gtgcatcttt cgtactgccg ggacgctttg gggctgcaaa gagtgcctac
     5401 ccgggcccgg gtgtctctcc ggcgacttcc cggcgcgcgc tcctctgcat catcgcggca
     5461 aatatgacgg tcccgatgag gctgatcgcg ccgcccacgt agaagacggc ctcgataccg
     5521 acgaagtcca tgaacgcgcc tcccatcagc ggcgcgatga tcatgccgat gccgaacacg
     5581 gtattataag cgcccatcga tgtccccata ccgatcgtcc gacccgcatc gaccgtcagg
     5641 gccatgatgg ccggctgctg gatggcgcct ccgaccccta tgagggaggt gaccaggagc
     5701 aggggcagga acgaatgggt cagggggatc acggcaagcg cgatcgccgc tatggtcgac
     5761 cccgcgacga tcagggcgac cttgttcccg gtatccgtga gcctcccgat aggcacctgg
     5821 aggagcgcca tcaggaaggt gtttgcggag aggactatcc ctacctcgaa cgggcttatg
     5881 gcgatcagcg ggccgaagac cgggacgaag accatcatac ccccgcgacc gagggcgctg
     5941 atgaaggcaa agaccatgac cccctgcatg accggcaacg tgatgagtgc gggcatcggg
     6001 gtgctcgggg catccgggag agcgaaaccc cccggttttg cctccgggag ggatatggcg
     6061 acgaggagcg tggcaaacgc cgagaggccg gccatggcgt agaagaccga cggcatgccg
     6121 aacgtatcgt ttaagaaccc tccgaggagc ggccccaccc ccataccgag gaacatcgat
     6181 atggagaagt tccccatgtg gctcccttct tttcccttct ccgagaggtc ggcaatgtag
     6241 gccatggcta tcgggacgac catggccgac gccaggccgt gggcgaagcg gacggccgtg
     6301 agcgaataga cgctgccggc gatgaggtag gcaagcgaaa ggaccgcgta ggcgaacatg
     6361 ccggtgagga tgatccactt cctcccccgc ctatccgata tccggccgat gatgggcatg
     6421 aagaccgacc gggagagagc gaaggctgaa aagatgacgc cgagccagat gccggtcgct
     6481 ccgaggttct ccgcgtagat cgggaggagc gggctgacga tgccgagacc gagcatcgtg
     6541 gcaaagacgg atatgaagag gacgttatag attcttctgg cggtattcat ggagatggca
     6601 tccggtcgga ggtgagatcc gcatgggatc gatcgtttgc aaatgcagca gaaagagtgg
     6661 gatataattt accatatata ttagcccggg tggccggggt tacccccgcc gcgcgacgca
     6721 gcaccaccgg tcgtatatgg ggtcgcgcgg attctcaaag acccgggtat cccacccctc
     6781 gccggcgcac cacccctcca cccgggtggc ctcggcctct gtgccgacgg tgatcagggc
     6841 ctcgtccgtg agggcaagga gctcggcgat gatcgatctc cagaggtcgg cgttgtagga
     6901 gtagatctcc ccggccatga acgcaacgcc gaggggcacc ggcgcgatgt agcgggaagc
     6961 ctcctgcgcg tcgatacaga tggtatcggc aggcgcgagg cggccccggg taagcccgag
     7021 tgcgaggagc gcggggtcgt tgtcataggc gagcgcccgc accccaccag cccggagggc
     7081 cgcggtcccg acgcccgagc cgcagcagca gtcgaggcag ggggttcccg cacggtcgcc
     7141 ccagaccacg gttacgaggt cgcggatatt cccctcccgg tcgggacgca ggtcttcgag
     7201 agcgggaaca acgtttgcgc ggatatcgag actgtaatac tcgcggactg ccgagagcca
     7261 tgcctcccgg ctctcctggt agatatcacc gccgacggcc tcgaagcact cgatgacctc
     7321 gatcgtcggg tccctgaaga ggaacgaagc ggcgtgccag ccgttcgaac cgtcgtggag
     7381 cgcgaacgcg agcggctcct cctcctcgtc gaccaggagt cgggcttcgt ccgggcttcc
     7441 ctggaacagc agggctgtac aggcgtctat cgtcagttcc gcaaacgacg gttcaaccaa
     7501 ccggcacccg tcgacctcaa ggatatctgc aaccttcata tcgttcgcct ctcgatcgtt
     7561 aagccacccg gcgcccggat gaccccgtcc acgcgggcgt cctcgtgcag ggtgaggccc
     7621 cggcaggaga tgttcccgag gatatgcacc tcctgctcga tgacgacgtc gccgtcgctc
     7681 tggacgtcgc cgtggatgac cgatctgcga gcgactttcg cgccttcccg tgcgtgcagg
     7741 ctcccaaaga tcgtcgtctc ttctcgtatg gcgatcgatg ccgcacggat gttcccgtgc
     7801 agccggcacc ccgacccgac ggccatcctc tcaggcaccg agaaggtctg catgttgagg
     7861 acggcgccgg ccgggatcat cagcggcgcc tccgtgcgga cctcgtcgtc gccgaagagt
     7921 ttctggagga cggcatccag gtcctcctgc ttctcgatac cgaggacggc cacgaggtac
     7981 atcatgatgt agacgatgac cggcatcggg ttacggatgg atatccagcc tttcgcctcg
     8041 aacccgcgct cgatctggac gttgtctccg atatcgaggt cgcctctgac gacgagcttg
     8101 ccccggatct tcaccccctc gccgaggtag gcgtcctcct ctgcgatgac gtcgccgccg
     8161 acctcgcacc agttgtctat ccggacgtcg ccgcccgcga cgatggtgcc gttgatcttg
     8221 ctgaattcac agaccacgac gtcgtttccc gcaaggccgt aatcgatccg gcaccggtca
     8281 ccgatgacga tatcctgcgc ggtcttgagc gtcctctcct ggagctcggt gtggtccggg
     8341 agggtgcagc ccttgatcca gtcgtggtcg ccagtctgtt ccatttattc ggtataatct
     8401 tcatgcgcca tacttatgac tcttattaat tagatcgagg gcgatcggca tgagatcgcg
     8461 gccgcggatg cggtgaaggc cgccctctgc gcacgagacc tcatcaaacc gggctacccg
     8521 atccgtccgg acccccggac ccctgatgac gaccggcacc gggtcggcgc tgtgatcctt
     8581 gatcgagcag ggggtgctgt ggtcggcgca gacgacgagc agggtatccc cgagatcgag
     8641 gagcggttcg agcgccgcat cgatcacctc gatgaagtcc cgcttctgga tgcccctccc
     8701 gtcgtggccg gcctcgtccg cgcccttgat gttcaagaga acgaagtctt tgcggtcgag
     8761 ctccgcaagc gccgccttca ccttggcgtc gaggtcggag tcgaccgacc cggtcgtccc
     8821 cggcacgggg atgtgctcga gccccacgac cttcccgatg ccggcgatga gggtggccgc
     8881 ggatatgacg ctcccggaaa gaccgtaacg actggagaac tgcgggaacg ctcccatctt
     8941 tccggcgccc cggatcagga ggaggtttcc cggcgcgagc ccctcttcca tccgccggac
     9001 gttcatcggg tggcgagaga ggatatcatg ggactggcgg atgaactcgt tgcaggcctc
     9061 cgccgtcttt ctatcttccg gatcgtcggt gcagggccgg atggcgggcg gccgcacccc
     9121 atcgcgcttc gggtcgttcg aggagacctt atcgccaagc ccacttcctg cgagagcgag
     9181 ggcggcccgg tggccggcgc ccgactcgaa cctgaacccg acgccgagag gcgagaggtc
     9241 gacgccgtcc cggatcgcct ccgcgagcgg tgctgtcccg gatatccgcc cggccctgcg
     9301 gtcggtgaca cggccctccg cgtcggtcgc ggcgaagttg caccggaaac cgatcatacc
     9361 ggcggtcatg cggatgccgg tcccttcggc ctcgagcggg ccgcggccgg tgtagtactc
     9421 ctgcggcggg tagccgagga gggcgaggtg cgaggtgtcg gagcccgggc gtatgccggg
     9481 agcgatggag tccatgatgc cgcagacgcc ctccgcggcg agccggtcga ggaccggggt
     9541 ccgtgcggcg gcaagagggg tccgtccatc cagcacatcg cagggacggt cggatatacc
     9601 gtccagtacg aggaagagca ccttctgagc aatcatcaga gactggtgcg atccggaacc
     9661 tgattactct tcccggtcac cggagcgata gacacctctc cgcgtggcgg agggcctcct
     9721 ttgcgagggc gtcgccccgc ctgcagcact cctcgctgca accgatcatg acgaggatgt
     9781 tcgtcgtcgt gatcatgtcg gccgagaggc accggatatc gatgacgatg tcgtcgccga
     9841 cgatcacctg cacgtcgcag acgacgtcgt ggagctggtg cgcataccgg tcgagcagcg
     9901 cgtgcgcggc gtcaagccag gcgtcgtcgc tctctttttc gcgaagagcg gtccactccc
     9961 cgtggaactg gacgagcatc ctcctgatga taccgaaggc catcttctgc tgctcctccg
    10021 ggggtcccgg gcagagcgcc atctctctta cctgccgcgc atccgtatgc tcttcctcgg
    10081 ccgtcaccgt ccctccggct gaggtgtacc cggtcatagg gtatataccc ttcgcggcat
    10141 ctccgtcgcc cgcgaacact acggatgcag gcggctaaaa ataagggtga gcagccgacc
    10201 ttatacggcc gcgatctgct cgagtttgga tttggtgatc tcgacccggc ggacggggta
    10261 gatcgtcttg acgaccttga agacgtcgcg ggccatgtcg ccggagacga tctccttgac
    10321 cagggtctca aagtcgctct ccgctgccct ggcggagagg gcctgcgata tcgcctggcg
    10381 cacggcgtgg acctggctct gttcggccct cgatatggtg agacagacga ccgtcacgcg
    10441 caggagttta ccgtccttgc tgacgatcgg gtggatggtg tcgatacggg acgcccgccg
    10501 tttgaccatc gaccggaggt agtcccgggt cacctcgtgc ccgacgaact cggtatacgc
    10561 ggcgtcgccg gccacgttat tgatcttgaa tctcatcttg atgtgggact tggagtagtc
    10621 ctgcaccact tcgccgagcg tcgcggtcat gacccggccg accatgttcg cggggtcggc
    10681 cgatatggta tcgccgatct cgactttgcc gaaggtctcg ggtacgtaga cgcggtacca
    10741 cttcttggcc ttccagcctt ctactcttct tccaacctgt ttcttccttg ccatggtatc
    10801 acttcactta ctctatgtcc ttgttctgag ccgcacgttc actccggacg gggaggttac
    10861 agatctcctc cgcgatcgcg aggttcatga ggtagtcgtc caccgatgct atggtcgacc
    10921 ggagcctggt cccgccgatc tcggcccgca cccctcccct gacggggtac gtcctgatca
    10981 gggtgaggtt gtcgggctcg agcgccgccg ccacgcaacc gggaaggctg tgcggcgtct
    11041 cgatggtccc ctcgatccgg gtcatgcggg catcgcctcg cgcagtccct gcctgaaccg
    11101 gtccacctgg tcggccccga tatgggcgcc ggccctccgg tggtgtccgc cgccccgtcc
    11161 gccgcacgcc cctgcgagcc tccgcatcag cgcatcgagg tcgaggtcga cgtccggcgg
    11221 gcagcgggcc gatacggagt agccgtcccc gcttaccccg atgacgaaga cggggccggt
    11281 tgcagggaaa tcgcgcgcga gtgcatccgc gacgtcgccc gtcaccgtcc cgtcgtccac
    11341 tgtgaagagg gcgagacgat cgtcgaggcg gcttgccccg cggatgccgg cgacgaccgc
    11401 gcgccggtgg cggacggtta catcccaggc ctcctggaga gcgtgggtcg agcggaggca
    11461 gagcgatgca ccgatatccc cgcacccggc cttgccgcag gcatcgacga cggcggcaag
    11521 gttccccgcc tcgtcgatca cctctcgccc gaggctgtag gtggtgcccc atatgccgca
    11581 gagcgcggta tgcgacgctt tcggggcggc cgtaagaacg acccgggaga ggagggcctc
    11641 gatatccacg tcgtcctcgt cggtgacccc ggcaaccagg gcccgggcgg cgtcgggttc
    11701 gcccgagagc ccggtgaggt aggggctgac ggcgagagcg agctgctcga cgagacctcg
    11761 cccgggcagg cgaaggccgc ggcggggcgt gatgaacccg tttgctatgc cttcgttcac
    11821 gatatcccgg ttcggcccct cgatatcctg gccgtcgccg agtatgccga ggagcgcgag
    11881 gcccgcgagg tcgcggttgt cccccatgcg ctgcgcgacg atgtaggccg tgccggccgc
    11941 ggagaggttc cggtcgccgt cgatcccggc gaggcgcggg ttgacgtggt aatcgccctc
    12001 gaagtggggc aggtggtggt ctaccaccat cacgtcgccg gggaggtcct ggagtgtcga
    12061 tccgaagtcg cagaggagca cgctgccctc ctccggcacg tcagccgcgg tgatgcccgg
    12121 gcgaatcctc agccggaacc gtccgccctt gcggaacatg gcgtggcaga ggatggatgc
    12181 ggcggcgata ccgtccgcat cgtggtgcgc gagcacttcc acgaactcct gctcgaggag
    12241 atggtcggcg agaccggcgg cagcagcgtc cagcgacatg gatcgtctca gcgggagagc
    12301 aggatctctg cagtctccgg tttgtaagtc cagcctttcg gcatccgtcc ggacctgacg
    12361 tagtacttta ccagcctgcg caccttggac tcggtgatct ggagctggcg agtgttgtgg
    12421 atgtcctttt tgttctcaga aaggtgcttt cttataccga gcgccttctg catcaggttc
    12481 cggagatcct cggggatctc ggaatcaagg cccttctcgc ggaggatctc ggttacacgc
    12541 ttgccggtgg cgagcttgac gtcagggacg ccgtaccggt cccgcaacgc aagacctatc
    12601 tgactgctcg acatgccgtc tttgcggaga ttgacgatga tcttctcgat ctccgctacg
    12661 tccgtgttgg accactcggg agcctccttc cggtagggcc ggacggaact gctggttcca
    12721 cggcgccggg cgtacattct tgccattcat acacctccga tatacactat gttaagtcca
    12781 gaaaaaagtc cgttgactga ctaatctctg taatcccgag cccttgcggg cagtgcttcc
    12841 gggagcacgt cggacttacc atagccagca catgagagtg ctgcatcata ttctccggga
    12901 tccgacttaa gtgtatcggg ggtgcgtgat cggtgttcag gcaattctcc actcgtgttt
    12961 tggtgcaccg gcgcgccatc cacttcctca aggtttatca tgccccgtaa tgaccattat
    13021 ggcatgatcg agagcgaacc ggaccgtatc gcggatggcg ttcgtttccc acaggagttt
    13081 cttcccctat tcggtatcgc cgcgtcagcg ctggttgcgc tcggcgtcac cgcgtactgc
    13141 ctcatctctg gaatattcat catcttcccc catctcttct acatcccgat catccttgtc
    13201 tcctactact acccgaaccg gggcgtcggg gccaccgtgg tgctctccgc cgcgtatctc
    13261 gcggagatcc tcttcctctc cccggtcagc gaggcggaga tcataaacgc cctggtccgc
    13321 atcggcgtgt tccttctcgt tgccgccgtc gtgtcgcacc tcgccggccg cctgcaggtg
    13381 cgggaggccc ggtaccgggg gatcttcgag acgtcgggcg cgggcatctt cctcttctcg
    13441 ccccggaccg gaaagatcga ggagatgaac cagcagtgcg ccgcgatgct cgggtaccgg
    13501 gaaggcgagg ctccctcctt tgatgtatcc acgatctggc ccgggtatac cggactcacc
    13561 ggaccccttg aggagggccg gatcgaaggc ctggactgca acctcgtcgc ccgggacggg
    13621 acgccctccg cggttctcct ctcggcaagc ctcctccccg accagcagga gggctgcgtg
    13681 gtggtcaccg gaaccgctga actgaagcag atggagaatc agctccgccg ttcaaaggag
    13741 acgctccggg tcatcctcga caccaccgat gtcgggatcc tcctgaccga cccgggcagg
    13801 acgatcgtgg aggcgaatgc ggccgcggtc cggttcttcg gcggagccgg acgggaggat
    13861 ctgctcggga aggatccata cgatctggtt gccgggaggg accgggaggc ctgggcggcc
    13921 tgccgggagc gggcgctgca cggggaggcg catgtctttg gggagtgcac gctctgcagg
    13981 ctcgacggca ccgagtggcc cgcggagttc acgatcacct ccttcgcaca gggcggagcc
    14041 gccccgggac gtctggtcgt ctcgttccgg gacatcaccg aacggcgccg ggcggagagg
    14101 gagatgcggg aggagaaccg gcgtctcttc gtcacgaacg aggtcatggc cgcggcaact
    14161 gcgtcccgcc gccttgacga cctcctcccg gccgcgctcg aaaaatctct ggcgctgctg
    14221 gacttcgacc tgggtgcggc gcacctggta taccccgggg acgatcgtgc gcacctgcgc
    14281 gcctcggtgg gcgatgtggt cttcccgctc gatgtgagcc gggatgaacc gccctaccgg
    14341 tacgtgctcg tcgacgggga ggcccgattc gtcgaccatt tccaggagaa gtatccggag
    14401 catggcaacc ccggcacccg atcgtttgca tcggttccca taccgggcga cgacgggccg
    14461 gtggggtgca tcacggtcgc gagcagagct cgcgagacga tcccggaggg cgagcgccgg
    14521 atcctcgtgg cgataggcga agggcttggg agtgccgtcg tgaaggggat gctccacgag
    14581 gacctggagg cggcgctcgc gtccgccaac cactatcttg agaaggcgaa cgccgcaacc
    14641 gaggcagcca acctctacgt cgatatcctc acgcacgaca tcaacaacgc gaataccgta
    14701 gccatgggct acctgcacat ggccctcgag tcgggcgata aaccaccccg tgagttcctc
    14761 cgggagccgc ttgcagccat ctaccagagc agtgacatca tcaggaacgt cacgacgatc
    14821 cggagactgg aggccggtga tgttaaactt cggccggtac ggcttgaatc tgtgttccgg
    14881 gggatgcgca actacttcgc cgatgcccgc atcacctgtg aggggccgga tgcaacggtt
    14941 ctcgcagacg atctcatcga cgagatcttc tcaaacctga tcggcaacgc cgtcaagttc
    15001 ggggggccgg atgccgggat caccatcagc gtccgggagg agggggaggc ggtatcggtg
    15061 acggtcgccg ataacggccc cggtatccct gacgacctca aaccgcggat attcgagcgc
    15121 aaggtgcgcg ggacgacgaa gaagagcgga aaagggctcg gcctcagcat cgtcaggatg
    15181 cttgcggaac gctacggggg aagcgtccgg gcgggagacc gcgtcccggg ccggccggag
    15241 gagggggcgg ccataatggt gaccctgcgg cgttataagg ccggggagga gtaaaccgaa
    15301 tgtttatcac ctccccgggc aacatgtaat ggtaccggcg cgatagtagt ctagcggtag
    15361 gacaggggct tcccaagcct ctagcccggg ttcg
//
LOCUS       DAOSEE010000040        15119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1573_length_15119_cov_253.022570, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000040 DAOSEE010000000
VERSION     DAOSEE010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 15119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15119
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1573_length_15119_cov_253.022570"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..450)
                     /locus_tag="PLI76_04610"
     CDS             complement(37..450)
                     /locus_tag="PLI76_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61348.1"
                     /translation="MANNPYDEMLKNIAQLMGKILSEMPLQDPKIIGFTIISGVPEGA
                     PYLDLAGDEGAGFEVIEGDDCIYITATVNAQVQGAPYVTFQRESVTLCTGGDEETIIG
                     LNCEIDIPHSFYNVQHGVIDAVCRKRSTPGEGARP"
     gene            complement(440..1063)
                     /locus_tag="PLI76_04615"
     CDS             complement(440..1063)
                     /locus_tag="PLI76_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HOI61349.1"
                     /translation="MLEGDEVSRLLDILGNRNRRRIIELLRQKPCFVTEISERLVLSP
                     KAVIEHLQMMEREELLVSCQDERRRKYYYLSHDINVIVNLQKQDGITPPSVEEDQRAR
                     FTRNLSALRRMILARDELLENLEQLEREIESRFVEVVRTGGGFTADDGDLEIILALSH
                     AALTLAELEEVSSLSTDELKQRLGSLMHMGIVRRINDRYRIRGTYGE"
     gene            complement(1098..2564)
                     /gene="guaB"
                     /locus_tag="PLI76_04620"
     CDS             complement(1098..2564)
                     /gene="guaB"
                     /locus_tag="PLI76_04620"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOI61350.1"
                     /translation="MYIEKMQMETTFTFDDVLLEPAESWVEPDEADVRSRFSRNIPLY
                     IPLVSAAMDTVTESVMAITMAREGGIGVIHRNMPPEREVAEIRIVKQAEDLIEREVVA
                     VGPDSTVADVERVMKLHGIGGVPIIEGSKVIGIVSRRDIRAILPRRGEAKITEYMTRK
                     LITASEDVTAESALETMYANKVERLPVVDGEGCLVGIITMRDILEKRQYPRANRDANG
                     KLRVAAAVGPFDFNRAMLLVDGGVDALVVDCAHGHNMNVVNAVREIKASVAIDVVAGN
                     IATRQAAGALVDSVDGLKVGIGPGSICTTRIVAGVGVPQVSAIASVAEVARDAGVPVI
                     ADGGIRYSGDIAKAIAAGADSIMAGSLFAGTDEAPGRITTIKGRRYKQYRGMGSLGVM
                     SGGESSDRYFQKKEIGKTKFVPEGVEGVTPYVGRVSDVIYQLVGGLKSAMGYTGSKTI
                     GDLKKNGKFLRITSAGYGESHPHNIMITDEAPNYRLLD"
     gene            complement(2568..3257)
                     /locus_tag="PLI76_04625"
     CDS             complement(2568..3257)
                     /locus_tag="PLI76_04625"
                     /EC_number="4.2.3.153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(5-formylfuran-3-yl)methyl phosphate synthase"
                     /protein_id="HOI61351.1"
                     /translation="MQLLVSPSSIEEARSSLSADIIDVKKPSEGSLGANFPWIIREIK
                     TLAGRKPVSAAIGDNEYKPGTAALSAYGAAHAGADFIKVGLMFDGTDHAREVIGAVTT
                     AVKEEFPHKYVVIAAYADFQRMDTISPLAISPLVADAGADIAMIDTGIKDGKSLFEFM
                     DEEALTRFTEQNRGFGLKTALAGSLRFEDLDALKRIDPEIIGVRGMVCGGDRSATIRA
                     ELVEKAMMMLR"
     gene            3302..3982
                     /locus_tag="PLI76_04630"
     CDS             3302..3982
                     /locus_tag="PLI76_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOI61352.1"
                     /translation="MELILAVDLAGGLVVHGRSGDRAGYRPLTWGLAPSAEPETYIAA
                     LEPRYIYIADLESIQGRTPQDELVRRCTALVERCYLDRGIRSPADCTAVAGVTPVVGT
                     ETAAKAIEDLAAYRAGYLSIDVKGGRVLPWGIRPEEMLCHASEFSFEGCIILNIGAVG
                     TQRGLVREELEGMRACYPGRLLYGGGVAGTDDIRLLDDAGFDGAIIATAVHRGTVPLE
                     WVRRGHPC"
     gene            3976..4593
                     /gene="tmk"
                     /locus_tag="PLI76_04635"
     CDS             3976..4593
                     /gene="tmk"
                     /locus_tag="PLI76_04635"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOI61353.1"
                     /translation="MLITIEGIDGSGKSTLIARLRELIADLDPLFTREPGATWVGESV
                     RRAVAERMDPITEALLFCADHAAHIDGVIRPALDEGRLVVSDRYSDSRFAYQPVVLAG
                     ILPDPLLWLRRIHDGWSVRPDRTFLLVLPIEDAVARLDTEKKREYFENAAILARVQEN
                     YLDLAASDPARFVVVDALLPKEDVARFIADEIRTNVLSSRRHPRA"
     gene            complement(4550..7141)
                     /locus_tag="PLI76_04640"
     CDS             complement(4550..7141)
                     /locus_tag="PLI76_04640"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOI61354.1"
                     /translation="MSPSHQLPKNYDIEEVERRWQDTWRDEENYFDPDSTKPRFIIDT
                     PPPYPTGNFHIGNALNWFYIDFVARYKRMRGFNVMFPQGWDCHGLPTEVKVEETYGIT
                     KNDVPRERFREMCRDLTLGNIEKMRATMRRLGFSTDWSHEYVTMLPEYYKKTQASFLQ
                     MLKAGDIYQSEHPVNFCTRCETAIAFAEVNYAPRTTTLNYFDFDGVEIATTRPELLAA
                     CVAVAVHPGDERYRGMKGATLAVPIFGHRVPVIEDVAVDPEFGSGAVMICTFGDKTDV
                     YWWKQHSLDLRKAIDRQGVMTAVAGRYAGMSSDACRKAILEDMEEAGILKRREDLEQR
                     VGTCWRCKTPIEILSERQWFVRVHQKEILEAAKEITWLPEHMFTRLENWTESMEWDWC
                     ISRQRIFATPIPVWFCDACGGMILPAMEDLPVDPTADMPKAPCPRCGGTRFTGEKDVL
                     DTWMDSSISVLHVTGWDGSSRPPLFPAQLRPQGHDIIRTWAFYTILRSKALAGSHPWE
                     QILINGMVLGDDGFKMSKSRNNIISPEEVVGKYGADALRQWGAGGAAIGSDIMFNWND
                     VVAASRFQTKLWNIFRFVMGHLERGADPAAPVTELIDRWLLVRLSDTVAEATAAMEGY
                     QFDRALKAVREFAWNTLADDYIEIAKGRLYAEGGGRASAASALRTTMDVLCRLLAPII
                     PHFAEECYHTLTGGSVHKEAWPDFTYADDEARRHGDLLVKTVAELRRYKHDKGMALNA
                     PLGHVTIYAPEPVDDTGDAGRALAADAHWRTGTPRLEEVADGVDFNMAVIGPALRKQA
                     RGFMEAVRALPPDLLKNPPAAVTVDGEEVFVPPGSFTPKIAYQVAGEEVDVLTLGDVV
                     VTIGHSS"
     gene            7304..8500
                     /locus_tag="PLI76_04645"
     CDS             7304..8500
                     /locus_tag="PLI76_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HOI61355.1"
                     /translation="MTKPPGLVLASVLTLAFIGLAPLLQPAWLLTLLLIPFSLVLFLI
                     RDTRYVSLSIIVLAALYGFGWLSTFVFTCTLGIVVIGELAFRFTGGKQASYLYHLAAA
                     VGVSLAVMLYLRYSAPLVVMMGVVAAVLLRAALRGRDDVLMVEALGVAMTMFLFEEIN
                     FQVDLTILVVAAVIAFGFGYSSYRLRVADVSGLFSGAMIGIILIVFAGVPWFLIMLTF
                     FIVGAGATRYRYGDKEQLGVAQEHAGVRGYFNVFANGLVATGAAILYGVTGNPAFAAL
                     FMGSVASAAADTAASEIGVTGKTPYLITTLQPVPRGTNGGVTLRGEMAAVAAAALVAL
                     VAWMLGVADPRMAVVTVAAGFVGTNVDSLVGATLENSGRIGNSGTNLAATFFGGVFGM
                     LVYLVT"
     gene            complement(8714..9541)
                     /locus_tag="PLI76_04650"
     CDS             complement(8714..9541)
                     /locus_tag="PLI76_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61356.1"
                     /translation="MTLGSLSGAAGLLRRHPVLWSVGIVMGVLAVIDLVIPLYGGTFY
                     AEPLALLQALVMPFLAGGVYGTIRANSFSAGEFLQSGKTYYFRILLPALLIFFAVILT
                     AFLLAIPLTLLGAGAASGTMPLLLGVLVSIVFFTFFYDTVAVFEETNVFESIRRSVEF
                     VMNNLGNVLVFYLVNIVVLVGIGFAAFVAIAALLVDRLEPLTRLDPAELQAVTPQDLL
                     GLIGTEGIWTIAAVYAVVIVLFAAFLYAYKASFFRDHAASVPIQQGEYDEKGRWYKY"
     gene            complement(9544..10134)
                     /locus_tag="PLI76_04655"
     CDS             complement(9544..10134)
                     /locus_tag="PLI76_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HOI61357.1"
                     /translation="MGFVTRNTYYFDAPGAQNTADAARFAVERAQELGAKKIVVASTG
                     GRTALTFLDAVKGTDLRLIVVTHVVGFSKPGEWEFSREAAETLRAAGATIVTGTHALS
                     GLERAISRSPGLGGSSRTEAIAEALRRVVAIGLKVAVECVLIAADQGVVGVDEEVIAV
                     GGTLSGADTVCVIRPAHTATFFNLQVREIVAMPRNR"
     gene            10343..11512
                     /locus_tag="PLI76_04660"
     CDS             10343..11512
                     /locus_tag="PLI76_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOI61358.1"
                     /translation="MITLAIAGKPNCGKSTFFRAATLAQAEIANYPFTTIDANHGVAY
                     ARTTCPCREMRVPCENCLDGVRFVPIGLIDVAGLVPEAHKGRGLGNQFLDNLRQADAI
                     LQIVDASGGTDAEGNPVGIGSRDPEKDIEFLQYEFSMWMYGILSRNWAKLQRQAQARD
                     FSLAAAIADTFAGLGVTLEQIRDATDEAGIDLRTAGEEDLIRFCRELMAISKPMLIVG
                     NKADLAPQECLDRLAKHDVIFASAAGELALRMAAEGKFIRYLPGDQSFTPNPEANLSA
                     AQQAGLAKVAEFMRRFDGTGIQRALDAAVFDLLDRIVVFPVEDEHKLTDSKGRVLPDA
                     FLMKRGSTPRDLAYQVHTDIGEGFLYAIDAKTGMRVKDTLELKNGDIIKIVSVRK"
     gene            11612..11932
                     /locus_tag="PLI76_04665"
     CDS             11612..11932
                     /locus_tag="PLI76_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61359.1"
                     /translation="MSGEVYQAQVLRSFFDTITGTDRNLTRIYMCVVSLAKLRMEPPE
                     RMTFLVDQLRKSKQKRELSVDIIDYMCDAARDLELGTVRTAFGVKDINEVADEFSGIS
                     LDSL"
     gene            complement(12120..13217)
                     /gene="fbp"
                     /locus_tag="PLI76_04670"
     CDS             complement(12120..13217)
                     /gene="fbp"
                     /locus_tag="PLI76_04670"
                     /EC_number="3.1.3.11"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphate aldolase/phosphatase"
                     /protein_id="HOI61360.1"
                     /translation="MVKTTVSVIKADVGSFPGHSRTHPKLLEVAARRLKEAEGSTIID
                     SYVTHCGDDLELIMTHTKGEDNEEIHKLAWDIFMECAELAKEMKLYGAGQDLLADAFS
                     GNVKGMGPGVAEMEFEERGSDPVLVFMADKTEPGAWNYFLYKIFADPFSTSGLVIDPS
                     MHQGFTFEVHDVMEKRRILFNTPEESYSLLAYIGAPSRYVIKYVRKKDGTVGASTSTQ
                     RLNLMAGRYVGKDDPVMVVRAQSGFPAVGEVIDPFRTPILVAGWMRGSHHGPFMPVGV
                     GDANCTAFDGPPRVICLGFQVNNGKLIGPADMFDDPAFDRVRDRCCELSDVLRAHGPF
                     EPHRLSLEEMEYTTLPGVEKQFKDRWEDLPE"
     gene            complement(13549..14001)
                     /locus_tag="PLI76_04675"
     CDS             complement(13549..14001)
                     /locus_tag="PLI76_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOI61361.1"
                     /translation="MSDSPADIFEQLNELMKRLMEQGLKEQGDQNRSFAYGFKIVLHD
                     AGKTETPAKEADPVAEPEKASSEGTIEPIAEMYTTDDDVTVAIDLPGFEKENIHLSLI
                     NGTLTIIAGSSQLTSVEVPAVDAGSMQSNYKHGVLEVRFSRGKTIKID"
     gene            complement(14085..>15119)
                     /locus_tag="PLI76_04680"
     CDS             complement(14085..>15119)
                     /locus_tag="PLI76_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOI61362.1"
                     /translation="KAELAEAVEWPLKYPEIFASLDTEPPRGILLFGPPGTGKTLLAK
                     AVANESESNFISVKGPELLSKWVGESERGVRQVFRKARQAAPSIIFFDEIDALMPKRG
                     TYIGSSHVTESVVSQILTELDGLEELNNVVVLGATNRPDMLDDALLRPGRLDRMIYVP
                     PPDREGRKKIFEVYMRNKEILANDVDIDELVDRTEGYVGADIEALVREAKISAMREFI
                     AAMGKKSDDERKKAIGNVRITKRHFEEALSRVRGTLDIDRLEENERQSWQILYNQEQR
                     SALEDAISTINRARMRETGKSEQETRDLTQTLKDEVYRRKKDFGEIKRLTKELKTRIE
                     RPLPQAGMAF"
BASE COUNT         2591 a         4862 c         4661 g         3005 t
ORIGIN      
        1 tgtaaaaaga gaaggagggc gggatgcgca cccggttcag ggccgggctc cctcgcccgg
       61 ggtgctcctc ttccggcaga cggcatcgat gacgccgtgc tgcacgttgt agaaactgtg
      121 cggtatatcg atctcgcagt tgaggccgat gatcgtctcc tcatcgcccc cggtgcagag
      181 ggtcacggac tccctctgga acgtgacgta aggagcgccc tggacctgcg cattcaccgt
      241 tgcggtgatg tagatgcagt cgtccccctc gatcacctca aacccggcac cttcatcccc
      301 ggccaggtcg aggtagggcg ctccctcggg gaccccgctg atgatggtga acccgatgat
      361 cttcgggtcc tggagaggca tctcgctcag gatcttcccc atcagctgcg cgatgttctt
      421 cagcatctcg tcgtatggat tattcgccat atgtaccacg tatcctgtat cgatcgttga
      481 tccgcctgac aatccccatg tgcatgaggc tgcccagtct ctgtttcagc tcatccgtgg
      541 acaggctgct cacttcctcg agttccgcaa gcgtcagagc agcgtgagag agggcaagga
      601 tgatctccag gtccccatcg tcagcggtga accctccccc tgtgcgtacg acctcgacga
      661 acctcgactc gatctcccgc tccaattgtt cgaggttctc caggagctcg tcgcgggcca
      721 ggatcatcct cctgagcgcc gagaggttcc tcgtaaaccg tgccctctgg tcctcttcaa
      781 cggagggagg tgtaatccca tcctgcttct gcaggtttac gatgacattg atgtcatggg
      841 agaggtagta atactttcgt ctgcgctcgt cctggcacga gacgaggagc tcctcgcgct
      901 ccatcatctg caggtgctct atcaccgctt tcggactgag caccaggcgc tcggagatct
      961 cggtcacaaa acacggtttc tgcctcagca gttctattat tcgccgcctg ttccggtttc
     1021 ccaggatatc gaggagacgg gaaacctcat ctccctcgag catgcttact gatcgttagt
     1081 gtttttaata taaaaggtta atcgaggagg cggtagttcg gcgcctcgtc ggtgatcatg
     1141 atgttgtgcg ggtggctctc gccatagccg gcggacgtga tcctgaggaa cttgccgttc
     1201 ttcttcaggt ctcctatcgt ctttgacccc gtgtagccca tcgcggactt caggccgccg
     1261 acgagctggt agatgacatc cgagaccctc ccgacgtagg gggtcacccc ctcgacgccc
     1321 tcggggacga acttggtctt cccgatctcc ttcttctgga agtagcggtc gcttgattcc
     1381 ccgccgctca tgacgccgag cgatcccatg ccccggtact gcttgtagcg ccggcccttg
     1441 atcgtcgtaa tcctccccgg agcttcgtcg gttccggcaa agaggctgcc tgccatgatg
     1501 ctgtccgcgc cggcggcgat tgcctttgca atgtcgccgg agtagcggat gcccccgtcg
     1561 gcgatcaccg gcacgccggc atcgcgcgcc acctcggcga cgctggctat cgcggagacc
     1621 tgcggcacgc ccacgcccgc gacgatcctc gtcgtgcaga tggaacccgg cccgatgccc
     1681 accttcagcc cgtcgacgga gtcgaccagg gctcctgccg cctgcctcgt cgctatgttt
     1741 cctgccacga catcgatggc gacgcttgcc ttgatctccc tgaccgcgtt gacgacattc
     1801 atgttatggc cgtgagcgca gtccaccacc agggcgtcga cgcccccatc gacgagcagc
     1861 atggcccggt tgaagtcgaa aggccccacc gcggcggcga ccctgagttt cccgtttgcg
     1921 tcgcggttcg cgcgggggta ctgccgtttc tcgagaatgt cgcgcatcgt tatgatgccg
     1981 accagacacc cctcgccgtc cacgaccgga aggcgttcga ccttgtttgc gtacatcgtc
     2041 tcaagcgcgc tctcggccgt gacgtcttca gacgccgtga tcagtttccg ggtcatgtac
     2101 tccgtgatct tcgcctcgcc gcgccggggc aggatcgccc tgatgtccct gcggctgacg
     2161 ataccgatca ccttgctccc ctcgatgatg ggcacgcccc cgataccgtg cagcttcatg
     2221 acccgttcca catcggcgac cgtggagtcc gggccgaccg ccacgacctc gcgctcgatg
     2281 aggtcttcgg cctgtttgac gatccggatc tcggccacct cgcgttccgg cggcatattc
     2341 cggtggatga ccccgatgcc gccttcccgg gccatcgtta tcgccatcac cgactcggtc
     2401 accgtatcca tggcggcgct cacgagaggg atatacaggg ggatgttgcg cgagaaccgc
     2461 gacctgacgt cggcctcgtc gggctcgacc caggattcgg cgggttcaag cagcacgtcg
     2521 tcgaacgtaa acgttgtctc catctgcatc ttctcgatat acatacatca cctgagcatc
     2581 atcattgctt tctcgaccag ctcggccctg atcgtggcgg atcgatcgcc cccgcagacc
     2641 atcccccgga cgccgatgat ctcgggatcg atgcgtttca aggcatccag gtcctcgaac
     2701 ctcagcgaac cggcaagggc cgtcttcagt ccgaaccccc ggttctgttc ggtgaaccgg
     2761 gtcagcgcct cctcgtccat gaactcaaag agactcttcc cgtctttgat gccggtatcg
     2821 atcatggcga tatccgcccc ggcatccgcc accagcgggc tgatggcgag cggagagatc
     2881 gtgtccatcc tctgaaaatc ggcgtaagcg gcgatgacga cgtatttatg gggaaactcc
     2941 tctttcaccg ccgtggtcac cgccccgatg acctcgcggg catggtcggt gccgtcgaac
     3001 atcaggccga ccttgataaa atcagcaccc gcgtgggcgg cgccgtaggc cgagagagcc
     3061 gcggttcctg gcttatactc attgtccccg attgcagcac tgacaggctt cctgccggca
     3121 agggtcttga tctcccggat gatccagggg aaattcgccc caagggagcc ctcggagggt
     3181 ttctttacat cgatgatgtc agcggaaagc gagcttcttg cctcctcaat gctgctcgga
     3241 ctgacgagta attgcataga aattaatgat ccttcaccct aatagtgatt gcgttgagga
     3301 catggaactg attcttgcag tcgatctggc gggcggtctt gtcgtgcacg gaagatcggg
     3361 cgaccgcgcc ggctaccgcc ccctgacgtg gggacttgcc ccgtcggccg agccggagac
     3421 ctacatcgcc gccctcgaac cccggtacat ctacatcgcg gacctcgaga gcatccaggg
     3481 cagaaccccg caggacgagc tcgtccggcg ttgcaccgcc cttgtcgagc gctgctacct
     3541 cgaccggggc atccggtcgc ctgccgactg cacggcggtc gccggggtga cgccggtcgt
     3601 cggcaccgag acggcggcaa aggcgatcga ggaccttgcg gcataccggg ccggctacct
     3661 cagcatcgac gtcaaaggag gccgggtgct cccctggggc atccggccgg aggagatgct
     3721 ttgccatgcg tcggagttct cgtttgaggg gtgcatcatc ctcaacatcg gcgccgtggg
     3781 gacacagcgc ggccttgtcc gggaagaact cgaggggatg cgggcgtgtt atcccggccg
     3841 cctcctctac gggggcggcg tcgccggaac ggacgacatc cgtctccttg atgacgccgg
     3901 gttcgatgga gcgatcatag caaccgccgt ccaccgcggc acggtgccgc tcgagtgggt
     3961 ccggaggggg cacccgtgct gatcaccatc gaggggatcg acgggagcgg caagagcacg
     4021 ctcatcgccc gcctccgcga gctgatcgcc gatcttgacc ccctcttcac ccgcgaaccc
     4081 ggcgcgacct gggtgggcga gtcggtgcgg cgggcggttg ccgaacggat ggacccgatc
     4141 accgaggcgc tgctcttctg cgccgaccac gccgcgcata tcgacggggt gatccggccc
     4201 gcgctcgatg aggggagact cgtcgtatcc gaccgctact ccgactcccg gtttgcctac
     4261 cagccggtcg tcctcgccgg catcctcccc gacccgctcc tctggctccg ccggatccac
     4321 gatgggtggt cggtccggcc tgaccggacg ttcctcctgg tcctccccat cgaagatgcc
     4381 gttgcgaggc ttgatactga aaaaaagaga gagtacttcg agaacgccgc catcctcgcg
     4441 cgggtgcagg agaactacct ggaccttgcg gcgtccgacc ccgcgcggtt cgtcgtcgtc
     4501 gacgcgctgc tcccaaaaga ggatgtcgcc cggttcatcg ccgacgagat caggacgaat
     4561 gtcctatcgt cacgacgaca tccccgagcg tgaggacatc cacctcttcg cccgccacct
     4621 ggtaggcgat cttcggcgtg aaggagccgg gcgggacaaa aacctcctcg ccgtcgacgg
     4681 tgacggccgc gggcgggttc ttgagcaggt ccggcgggag cgcccggacg gcctccatga
     4741 acccgcgggc ctgcttcctg agcgccgggc cgatgaccgc catgttgaag tccacgccgt
     4801 ccgcgacctc ttcgagccgg ggtgtaccgg tgcgccagtg ggcgtctgcg gcgagcgcac
     4861 ggccggcgtc gccggtatcg tcgaccggct ccggcgcgta gatcgtcacg tggccgagcg
     4921 gggcgtttaa ggccataccc ttatcgtgct tgtagcgccg gagttcggcg accgtcttca
     4981 ccaggagatc gccgtggcgc cgcgcttcgt cgtcggcgta cgtaaagtcg ggccaggcct
     5041 ctttatgcac gcttcctccg gtcagggtat ggtagcactc ctcggcgaag tgcggtatga
     5101 tcggggcgag gaggcggcag aggacatcca tggtcgtccg gagcgcgctt gcggcgctcg
     5161 ccctgccgcc cccctccgca tacaggcggc ccttggctat ctcgatgtag tcgtcggcaa
     5221 gggtgttcca ggcaaactcc cggacggcct tgagcgcccg gtcgaactgg tagccctcca
     5281 tcgcggcggt ggcctccgcg acggtatcgg agagccggac gaggagccac cggtcgatga
     5341 gctccgtcac cggcgccgcc ggatcggcac cccgctccag gtgccccatg acgaacctga
     5401 atatgttcca gagcttggtc tggaaccgcg atgcggcgac gacatcgttc cagttgaaca
     5461 tgatatccga gccgattgca gcccccccgg caccccactg ccggagcgca tccgccccgt
     5521 acttcccgac gacctcttcg ggggagatga tgttgttccg gctcttgctc atcttgaacc
     5581 cgtcgtcccc gagcaccatg ccgttgatga ggatctgctc ccagggatgg ctgccggcca
     5641 gggctttcga ccggaggatc gtgtagaacg cccacgtccg tatgatgtcg tgcccctggg
     5701 ggcggagttg cgccgggaag agcggtggtc tgctgctccc gtcccacccg gtgacgtgga
     5761 gcaccgatat cgacgagtcc atccaggtgt cgagcacatc cttctcgccg gtgaaccggg
     5821 tgccgccgca ccgcgggcag ggggctttcg gcatatcggc ggtcgggtcg acggggagat
     5881 cctccatggc cgggaggatc ataccgccgc aggcgtcgca gaaccagacc gggataggcg
     5941 ttgcgaatat ccgctgccgg gatatgcacc agtcccactc catcgactcc gtccagttct
     6001 caagccgggt gaacatatgc tccggcagcc aggtgatctc tttggccgcc tcgaggatct
     6061 ccttctggtg gacccggacg aaccactggc gctccgagag gatctcgatc ggtgtcttgc
     6121 accgccagca ggttcccacc cgctgctcga gatcttcccg gcgcttcagg atacccgctt
     6181 cctccatatc ctcaaggatt gcctttctgc aggcatccga cgacatcccg gcgtagcgcc
     6241 ccgcgacggc ggtcatgacg ccctgccggt cgattgcctt ccgaaggtcc aggctgtgct
     6301 gcttccacca gtagacatcc gtcttatccc cgaacgtaca gatcatcacc gcgccgctgc
     6361 cgaactccgg gtcgaccgcg acatcctcga tgaccggcac gcggtgcccg aatatgggga
     6421 ccgcgagcgt tgctcccttc atgccgcgat accgctcgtc gccggggtgg accgccaccg
     6481 cgacgcaggc cgcgaggagc tcgggccggg tggtggcgat ctcgacgccg tcaaagtcga
     6541 agtagttgag cgtggtcgtg cggggagcgt agttgacctc ggcaaacgcg atggccgtct
     6601 cgcaccgggt gcagaagttc accggatgtt cgctctggta gatgtcgccg gctttgagca
     6661 tctgtaaaaa cgacgcctgg gtctttttgt agtactccgg gagcatggtg acgtactcgt
     6721 ggctccagtc ggtggagaac ccgaggcgcc gcatggtcgc ccgcatcttc tcgatgttcc
     6781 cgagcgtgag gtcgcggcac atctcccgga acctctcccg gggtacgtcg tttttggtta
     6841 tcccgtaggt ctcctcgacc ttcacctcgg tggggaggcc gtggcagtcc cacccctgcg
     6901 ggaacatgac gttgaagccg cgcatacgct tgtaccgcgc gacgaagtcg atgtagaacc
     6961 agtttagggc gttcccgata tggaagttgc cggtggggta gggcggcggc gtatcgatga
     7021 tgaaccgggg tttggttgaa tcgggatcga agtagttctc ctcatcccgc caggtatcct
     7081 gccagcgcct ctccacttct tcgatatcgt aatttttggg taattgatgt gacggcgaca
     7141 ttttcagcgt atgatctctc tcgttcctgt gaaatatatt gttcgacgga ggtttggcgc
     7201 ccccgggcct gccgtgcgca ccggggctct cacatcccgg agagggaaga gggcggatac
     7261 tcgaccctaa aggctttttc cctgcaccgc caaaggtgta tgaatgacaa agccgccggg
     7321 gttggttctg gcatcggtgc tcaccctcgc cttcataggc cttgctcccc tgctccagcc
     7381 ggcgtggctg ctcaccctcc tcctcatacc gttctcgctc gtcctcttcc tcatccggga
     7441 tacccggtac gtctcgctct cgatcatcgt gctcgccgcg ctctacgggt ttgggtggct
     7501 ctcgacgttc gtcttcacct gcacgctcgg catcgtcgtc atcggcgagc tcgccttccg
     7561 gttcacggga ggaaagcagg catcctacct ctaccacctg gctgccgccg tcggcgtatc
     7621 gcttgcggtg atgctctacc tccgctacag cgctccgctc gtcgtcatga tgggggttgt
     7681 ggccgcggtg ctgctccggg cggccctccg cggccgggac gatgtcctga tggtcgaggc
     7741 gctcggcgtc gcgatgacca tgttcctctt tgaggagatc aacttccagg tcgacctgac
     7801 catcctcgtc gtcgcggccg tgatagcgtt cgggttcggt tattcgtcct accggctccg
     7861 ggtggccgac gtcagcggcc tcttctcggg cgccatgatc gggatcatcc tcatcgtctt
     7921 tgcgggcgtg ccgtggttcc tgatcatgct caccttcttc atcgtcggcg ccggggcgac
     7981 ccggtaccgt tacggggata aagagcagct cggggtcgcc caggagcacg cgggcgtgcg
     8041 cggctacttc aacgtctttg caaacggcct cgtggcgacg ggtgccgcca tactctacgg
     8101 cgttaccggg aaccctgcgt ttgcagcgct cttcatgggg agcgtcgcct ccgccgccgc
     8161 cgataccgca gcaagcgaga tcggggtcac ggggaagacg ccctacctga tcacgacgct
     8221 ccagccggtg ccccggggaa cgaacggggg ggtgaccctg cggggcgaga tggcggccgt
     8281 cgccgccgcc gccctcgtcg ctctcgtcgc ctggatgctg ggcgtcgccg acccccgcat
     8341 ggccgtcgtc acggtcgccg ccggttttgt cggcacgaac gtcgacagcc ttgtgggcgc
     8401 aacgctcgag aacagcggca ggatagggaa ctcgggcacg aacctcgccg ccacgttctt
     8461 tggcggggtc tttgggatgc tggtctacct cgtgacgtga ggcgcggcga tctgcggcat
     8521 cgagctcgcg catcccctct ctcctgcact cacgttccgg tgcttccgta tccgggcact
     8581 cttcccagaa agatcgggga agagcgccag aggccgggat ctcgcccgaa agatccggaa
     8641 ctctccggga gccggcggcc cgcccgtttg aaccccgcgt aaaaagagag aatcggtgcc
     8701 ccggggcacc gcctcagtac ttataccacc ggcccttctc gtcgtactct ccctgctgta
     8761 tggggacgct tgcggcatgg tctcggaaga aacttgcctt gtaggcgtaa aggaaggccg
     8821 cgaagagcac gatcacgacc gcgtagacgg ccgcgatggt ccatataccc tcggtcccga
     8881 tgagcccgag gagatcctgc ggcgtcaccg cctggagttc ggcagggtcc aggcgggtga
     8941 gcggctcgag tctgtcgacg agcagggcag cgatggcgac gaaggcggca aacccgatgc
     9001 ccacgaggac gacgatgttg accaggtaga agacgaggac gttgcccagg ttgttcatga
     9061 cgaactcgac gctccgccgg atcgactcaa agacgttcgt ctcctcaaag accgccacgg
     9121 tatcgtagaa gaacgtgaag aagacgatcg agacgagcac tccgaggagg agcggcatcg
     9181 tacccgaggc ggcaccggcg ccaaggagcg tgagcggtat cgcgagcaga aaggccgtca
     9241 ggatgaccgc aaagaagatg agcagcgcgg gcaggagtat cctgaagtag taggtcttgc
     9301 ccgactgcag gaactccccg gcggagaacg agttcgccct gatcgtgccg tagaccccgc
     9361 cggccagaaa cggcatcacg agggcctgca ggagtgccag gggttcggcg tagaaggtcc
     9421 cgccgtagag ggggatgacg agatcgatga ccgcgagaac ccccatgacg atgccgaccg
     9481 accagaggac ggggtgccgc cggaggagtc cggcggcgcc ggaaagcgat ccgagcgtca
     9541 tattcaccgg ttcctcggca tggcgacgat ctcgcggacc tgcaggttaa agaacgttgc
     9601 ggtatgcgcc gggcggatga cgcagacggt atcggcgccg ctcagggtgc cgcccaccgc
     9661 gatcacctct tcatcgacgc cgaccacacc ctggtcggcc gcgatgagga cgcactccac
     9721 ggcgaccttc aggccgatgg caacgacgcg ccgcagcgcc tcggcgatcg cctcggtgcg
     9781 cgagctcccg ccgagtcccg gcgaacggga gatcgcgcgt tcaagacccg agagcgcgtg
     9841 cgtgccggtg acgatcgtcg cgcccgccgc ccggagggtc tctgccgcct cccgggaaaa
     9901 ctcccactcg cccggctttg agaaaccgac cacgtgggtg acgacgatca ggcgaaggtc
     9961 cgtccccttc acggcatcca ggaaggtcag cgccgtccgg ccgccggtgc tcgcgacgac
    10021 gatctttttc gcgccgagct cctgcgccct ctcgacggcg aaccgggcgg cgtcggcggt
    10081 gttctgtgca cccggggcat cgaagtagta ggtatttctg gttacgaagc ccatgcagtc
    10141 atgttgttcc cgctccttat tgaatccccc gttccggcgt tggaacgtgg ttttcaaggg
    10201 aggcggtgca atgtgcggga tcccgggcga cggagagatc accggttccg gcaaacggca
    10261 gatcccctgc gcacaatcca taaatggtgc ataacccaac actactctca cgccgaacgg
    10321 tgcagtataa caggagttgc tcatgatcac actggccatt gcaggaaaac ccaactgcgg
    10381 caaatcgaca ttcttcaggg cggcgaccct ggcccaggca gagatcgcca actatccgtt
    10441 cacgacgatc gacgccaacc acggcgtcgc atacgcgagg accacctgcc cctgccggga
    10501 gatgcgcgtc ccgtgcgaga actgcctcga cggagtccgg ttcgtcccca tcggcctgat
    10561 cgacgtggcg ggcctcgtgc ctgaggcgca caaggggagg gggcttggga accagttcct
    10621 cgacaacctc cggcaggcgg acgcaatcct ccagatcgtc gatgccagcg gcgggacgga
    10681 cgccgagggg aacccggtcg gcatcggatc gcgcgacccg gagaaggaca tcgagttcct
    10741 ccagtatgag ttctcgatgt ggatgtacgg catcctctcg cgcaactggg cgaaactcca
    10801 gcggcaggcc caggcacgtg acttctccct tgccgccgcg atcgccgata cgtttgccgg
    10861 ccttggagtg acgctcgagc agatccgcga cgccacggat gaggcgggta tcgacctccg
    10921 gactgccggg gaggaggacc tcatccggtt ctgccgggaa ctgatggcga tcagcaaacc
    10981 gatgctgatc gtcgggaaca aagccgacct ggcgccgcag gagtgcctgg accggctcgc
    11041 aaagcacgac gtcatcttcg cgagcgccgc cggggaactt gcgctccgga tggccgcgga
    11101 ggggaagttc atccgttacc tccccgggga ccaaagtttc acgccaaacc cggaggcaaa
    11161 cctcagcgcg gcgcagcagg cgggacttgc aaaggtggcg gagttcatgc ggaggttcga
    11221 cggcaccggt atccagcggg cgctggatgc cgcggtcttc gatctcctcg accggatcgt
    11281 cgtcttcccg gtcgaggacg agcataaact caccgacagc aagggccggg tgctccccga
    11341 tgccttcctc atgaagcgcg ggtcgacccc ccgcgacctt gcctaccagg tccacaccga
    11401 tatcggcgag gggttcctct acgccatcga cgcgaagacc ggcatgcggg tcaaagatac
    11461 actcgaactg aagaacggcg acatcatcaa gatcgtcagc gtccggaagt gagcccggta
    11521 gaggggaagg gcggatcccc tctctttgtg cccttcccgg cgaacacaaa tcggcaggct
    11581 ttaatattgc accggggaat atccgatcac catgagcggc gaggtctacc aggcgcaggt
    11641 cctccggagc ttcttcgata ccatcaccgg gacggacagg aacctgaccc ggatctacat
    11701 gtgcgtcgtg agcctcgcca agctccgcat ggagcctccc gagaggatga cgttcctcgt
    11761 cgaccagctc cgcaagtcca agcagaaacg cgagctctcg gtcgatatca tcgattatat
    11821 gtgcgatgcg gcgcgcgacc tcgagcttgg cacggtccgg actgcgttcg gcgtaaaaga
    11881 catcaacgag gtcgccgacg agttcagcgg gatcagcctc gactcgctct gatcttccca
    11941 ccccgggttg ccggataatc cttctcgcga gcggcgggac cgtctccccg gcaaactccg
    12001 gcggcacctg ggcctgaatg gtcaataagc ctctgcgaga gacgacggtc tgcagccggc
    12061 ggtgcgcgcg agcgacaccg gggaggtaga gaggcctccc ggtgtcctgc aaaaaggggt
    12121 tactccggga gatcttccca gcgatctttg aactgcttct cgacgccggg gagggtggtg
    12181 tactccatct cctcaagcga gaggcggtgc ggttcgaacg gcccgtgggc cctgagcaca
    12241 tcggagagct cgcagcaccg gtcgcggacg cggtcgaacg ccgggtcgtc gaacatatcc
    12301 gccggcccga tcagcttccc gttgttgacc tggaacccga ggcagatgac ccggggcggt
    12361 ccgtcgaacg cggtgcagtt tgcgtcgccg acgcctaccg gcatgaacgg tccgtggtgc
    12421 gaaccgcgca tccatccggc cacaagaatg ggcgtcctga aggggtcgat gacctcgccg
    12481 accgccggga acccgctctg cgccctgacg accatgaccg gatcgtcttt gccgacgtag
    12541 cggccggcca tcaggttcaa ccgctgggtg ctcgtggagg ccccgacggt gccgtccttc
    12601 ttccggacgt acttgatcac gtagcggctc ggtgctccga tgtaggcaag gaggctgtag
    12661 gactcttcag gcgtgttgaa gaggatcctg cgcttctcca tgacatcgtg gacctcgaag
    12721 gtgaacccct ggtgcatcga cgggtcgatg acgaggccgg atgtgctgaa ggggtcggcg
    12781 aagatcttgt agaggaagta gttccacgcc cccggctcgg tcttatcggc catgaagacc
    12841 agcaccgggt cagaaccccg ctcttcaaac tccatctcgg cgacgccggg ccccatcccc
    12901 ttgacgttgc cgctgaacgc atctgcgagc aggtcctggc cggcgccgta gagtttcatc
    12961 tctttcgcaa gctcggcaca ttccatgaag atatcccacg ccagtttgtg gatctcctcg
    13021 ttgtcctcgc ccttcgtgtg cgtcatgatc aactcaaggt catcaccgca gtgggtgacg
    13081 taggagtcga tgatggtgct gccctctgcc tctttaagtc tccgggcggc cacctcgagg
    13141 agcttcgggt gggtgcggga gtgccccgga aaactgccga catctgcttt gatcacggat
    13201 acggtggtct taaccattta atcaccattg cctggaaagg tgtagccctc aagactacta
    13261 gataggtatt cgtgtatatg taggtgttgc tgcctagaaa aagggttggc tcctgcaacc
    13321 gatcttacgg ataggctttc tccccccgca aaccgatcct ccgtggcaga ttcgcccggc
    13381 ggctattccg gggatgattt gatgttgctc atcgctttcg tcgtcgaaat caggtctatg
    13441 aacggtccgg gcatcgccgt gagcagattc tctggtgccc tcgtcgggag gcccggatac
    13501 gacaagaaga ggggtggggt gcggcctctc cgggaggttg cccgccgatc aatcgatctt
    13561 gatcgtcttg ccccgcgaga acctgacctc caggacgccg tgcttgtagt tcgactgcat
    13621 cgaaccggca tccacggccg ggacctccac cgaggtcagc tggctgctgc cggcgatgat
    13681 cgtcagagtg ccgttgatga gtgacaggtg aatgttctcc ttctcgaaac ccgggagatc
    13741 gattgccacc gttacgtcat cgtcggtcgt gtacatctct gctatcggct cgatggttcc
    13801 ttcggaagat gctttctctg gctctgctac cggatccgcc tcctttgccg gggtttcggt
    13861 cttgccggca tcgtggagga cgatcttgaa cccgtaggca aatgatctgt tctgatctcc
    13921 ctgctctttg agcccctgct ccatgaggcg tttcatcaat tcgttgagtt gctcgaagat
    13981 gtctgctggg ctgtcactca tgtacatcac gttgtctttg tccctgtcgg cgggggccgg
    14041 atgcccccgg gaaggaagcg cctcatgggt cgagggcgct cccgtcagaa cgccataccc
    14101 gcctggggca gcgggcgttc aatcctggtc tttaactcct tggtgaggcg tttgatctca
    14161 ccgaagtcct tcttcctccg gtagacctcg tccttgaggg tctgcgtgag atctctggtt
    14221 tcctgctcgc tcttcccggt ctcccgcatc cgggcacggt tgatcgtcga gatggcgtct
    14281 tcgagtgccg accgctgctc ctggttgtag aggatctgcc aggactggcg ttcgttctcc
    14341 tccaaccggt cgatatcgag cgtgcctctc acgcgggaga gggcctcctc gaagtgcctc
    14401 ttcgtgatcc tcacgttgcc gatcgccttt ttccgctcgt catcgctctt tttgcccatg
    14461 gcggcgatga actcgcgcat agcggagatc tttgcttcgc ggaccagtgc ctcgatatcg
    14521 gcgccgacgt aaccctcggt cctgtcgacc agttcgtcga tgtcgacgtc gtttgcgagg
    14581 atctccttgt tcctcatgta cacctcgaag atcttcttcc tgccttcccg gtcgggcggc
    14641 gggacgtaga tcatgcggtc caatctgccg gggcgaagca gcgcatcgtc cagcatgtcc
    14701 gggcggttgg tagcgccgag aaccacgaca ttgttcagct cctcaaggcc gtcgagctcc
    14761 gtcaggatct ggcttaccac actttcagtt acgtgcgatg atcctatata agttccgcgc
    14821 tttggcatga gtgcgtctat ctcgtcgaaa aagataatcg acggcgcagc ttgccttgct
    14881 ttcctgaata cctggcgaac accgcgttct gactctccaa cccattttga gaggagttcc
    14941 ggccctttaa cggagatgaa attgctttcg ctctcgtttg cgaccgcctt tgcaaggagt
    15001 gtctttcctg ttccgggcgg gccgaagagc aggatgcccc gggggggctc ggtgtcgagc
    15061 gatgcgaata tctccggata tttgagcggc cactcaacgg cctccgccag ttcggcctt
//
LOCUS       DAOSEE010000041        15112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1574_length_15112_cov_245.425384, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000041 DAOSEE010000000
VERSION     DAOSEE010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 15112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15112
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1574_length_15112_cov_245.425384"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..968)
                     /locus_tag="PLI76_04685"
     CDS             complement(174..968)
                     /locus_tag="PLI76_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HOI61363.1"
                     /translation="MDAFASLQRSIEKLVQIKCANYKEDYIKRRILSRMRLTNAADYE
                     AYHRYLLANPQEVNLLRNALTINVTKFFRDPEVFEVVRREVLPDLAGRGRSIRIWCAG
                     CSSGEEPYSIAILAHELTALRPDVSVMIYATDIDAVVLQKAMAGIYDAKALENVDERR
                     IRKHFVSHDDGTFEVRSHIKDLVKFRQHDLMSGIPITRYLDAVTCRNVTIYFTENQKN
                     ELARLFHSALGADGYYIMGKTEYLAREVEHLFSPYNIAQKILRKAA"
     gene            complement(998..1477)
                     /locus_tag="PLI76_04690"
     CDS             complement(998..1477)
                     /locus_tag="PLI76_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOI61364.1"
                     /translation="MAASVDVVEFQLGEEHYALDIQIAREIVEMMPITPLPRAPRYLA
                     GIINLRGEITNIIDLRDLLGLPGSERVESQKIVVLMPDVAGGSNVGIIVDDVHSVVSV
                     RDDQIERINDGITSSISNYVKAIIKRGSGGDGKAKTLIIWLDVQKVIGDLRHYQSMP"
     gene            complement(1488..4352)
                     /locus_tag="PLI76_04695"
     CDS             complement(1488..4352)
                     /locus_tag="PLI76_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOI61365.1"
                     /translation="MMIDKPGRKALFTGPVVPGTTGQERGPVVLPGAIESALRSGIGG
                     DGSARIDLAGVPEEFRPLATSINALLAKSEEEKQDLRTRLEAAGALRQRSETIIQENP
                     MPILIVDPQFRITVANTAYADMSGIPKGQALGKSLRDFKITSQKGSGLRQAVQEKKRA
                     YAEVIVELPSGTHTLEQYGIPLLDEEGAIESILIVYNDITRQREGEEEIKSQMENIAE
                     LQQRSDTIIQKNPMPILVVDTAMKILTANDAYLQMTGIDRSRIASMTLRDFKVLAQKG
                     EGIRKVLTEKVRVRGEVTVEFPAGTYTLEQYGIPLLNDKNELTGILNVYNDITEKRKE
                     EEEIRRMQHRIETMIKQNPLAIATLRPDKSRIDINDEYARMWRGTREETLAKKLYDYD
                     ITVLKGEHFYACYETKKPARTDVMVKWPDGVKKYLTLNAIPILDAKGDIEMAFYVWND
                     WTDLQGKEEEVKKTEHRVDAMIKQNPLAIATLRPDKSRIDINDEYARMWRGTREETLA
                     KKLYDYDITVLKGEHFYACYETKKPARTDVMVKWPDGVKKYLTLNAIPILDAKGDIEM
                     AFYVWNDWTRSKEEEDQIRALMETAKQEGENLAKSAEGLGSALAAMAKGDLTAFVDAG
                     ADDPLQKVKSDYNASLTAIRSLLAEVARAAHQVELTTREVSKSSDEIIRATEQVAVST
                     QRSSEDARRQLDKIEEIGKDINDLSASIEEIASTSQEVMEHASKASKEGNEAASLGEV
                     ATNKMLLVEGISKQTVDEISTLNNQMREINNIVKLITDIANQTNLLALNAAIEAARAG
                     EHGRGFAVVAGEIRNLAGESKRASQDIEDLIRTIQASTEKTAGSMKASHQEIQAGIES
                     VNKVIGGLSRIVNGVEIVAHGITEITKATEDQAGSTNNVMQKMDESTHMTKENLDRTE
                     DMAALAEEVSASTEEVGSAAHELAGMAEQLQKVMKQFKLN"
     gene            complement(4352..5233)
                     /locus_tag="PLI76_04700"
     CDS             complement(4352..5233)
                     /locus_tag="PLI76_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOI61366.1"
                     /translation="MLDITIVAFAHHKGGTGKTTSCLNVAGYLQKSGRSVLVVDCDPQ
                     ANATAGLGVDPDTLELSMYDLFMSAFEGFPDVVINDIIVPTASGVDLAPATLDLVGVE
                     PYLYSIEDRAGLLREALAGVRGNYDFILIDTPPSMGQFVINGLVAADHTIVTLDPGTF
                     ALRGMEALSTVFHDIREMLGENVTTDLAILTRWKGPDDRAAKTGGLALFLKRIFSSGP
                     SPEEERERERLKMFEAEVRKAFKQVFIVPYSPAIYETQQKGLPISHYAPESDAGREYR
                     AITTALLELDKNYGKDV"
     gene            complement(5365..6075)
                     /locus_tag="PLI76_04705"
     CDS             complement(5365..6075)
                     /locus_tag="PLI76_04705"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOI61367.1"
                     /translation="MDKADDDSRLHRIFRELQGKGLIGRERRLVQCPFTPSARGCPAL
                     NCIRYRRAFYERYLFGASDDTLRFVLLHEEGHIRSGSSLLSALPVLLALMLLPAALPF
                     LQAGGPLVRFASIALLAVTVFSIYRIYCRRMCDEEFAADRYAAEAMRCCYGVKDPGSL
                     LQNLLTGLRVEASAEPAPHRRTYGGLSRVIPLLLRPGPDYRPSIADRVQKIRAGVSAD
                     RLSSGRMRAPDTPVIPRE"
     gene            6403..6759
                     /locus_tag="PLI76_04710"
     CDS             6403..6759
                     /locus_tag="PLI76_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61368.1"
                     /translation="MSRYVGFLILALAALTVAAGCLSTGFGEVTYGGDALQVRVENTG
                     RPVENAVLQVTVTEVGALEQHEILSEARYVNLAPGENEYSVPVNLQPGTYRLFLTIFI
                     DGERKASVIRDLEVAP"
     gene            6756..8414
                     /gene="ade"
                     /locus_tag="PLI76_04715"
     CDS             6756..8414
                     /gene="ade"
                     /locus_tag="PLI76_04715"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HOI61369.1"
                     /translation="MTPAGLAAARGLEPADVVFSGGEVFNPFACAWERTDVAVSGGRI
                     VGLGDYEGKREYDLTGTYVVPGLIDAHVHIESSLLAPAEYARLVLRCGTTTVIADPHE
                     IANVCGAPGIEYMLAEGAQTPLDILVMLPSCVPATPFDLGGARLAAADLARFRGREGV
                     VGLAEVMNVPGVLAGDEDLRAKMDLFPVIDGHAPLLSGKDLNAYIYAGVQSDHECTAL
                     AEAREKLLRGMYVMIREGSTEQNLADLLPLVDACTAPRCCFATDDRHVDMLAGKGHID
                     DCIRRAVACGLEVELALRMATLSAAERFGLHDRGALAPGRLADFCIVDDPDRFRVLRT
                     FKRGIEVADAGYRPPACPAAPLHARVPAPRDIRIAGRGEARVIGIVPGQIVTRDLRCP
                     VDAADIPDTDRDILKVVVTDRYRGGGSGVGLVHGLGLREGALAGSVSHDSHNIVAVGV
                     ADADIVRAIGEVVRLGGGLAAVLGNEVTALPLECAGLMSALPYDEVARSLAALEEHAR
                     RLGAVENPFMYLSFLALTVIPATRVTERGVFDVTAFKDVPLFSE"
     gene            complement(8527..9606)
                     /locus_tag="PLI76_04720"
     CDS             complement(8527..9606)
                     /locus_tag="PLI76_04720"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOI61370.1"
                     /translation="MTEVTDVAWILFVISIVVLLALDLGVFNRKAHVIKPREALLQVA
                     VFIAAAVLFNIGIYTWMGQEAGLEFTTGYIMELMLSVDNLFVFVIVFASFCVPQEDQH
                     KVLFYGILGALLFRFAFIMAGVALVETFSWVLYIFGAFLIFTGIRMVTKKEEKAVEPD
                     KNILVRGFRKIMPVTGNYEGDRFFVRKTDAAGRAVLWATPMLVTLLVVETTDILFAVD
                     SIPAILGITTNPFIVFSSNAFAILGLRSMYFALAHIMSAFCYLKYGLAGILSFIGVKM
                     LVVEIYHVPVVVSLAIILVILGVTIIASLIRNRRTGTCPEIVSVQADACPALRSLRAD
                     DAPAPMAETARSISKEIAEVKKGKE"
     gene            complement(10048..10797)
                     /locus_tag="PLI76_04725"
     CDS             complement(10048..10797)
                     /locus_tag="PLI76_04725"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HOI61371.1"
                     /translation="MQGERYWEIDAARGIAVVTMIVFHSAFDLNFFGVLPLPVSGGFL
                     RMLAYMTASTFIFIVGVSFTISYARASQRLAGRDLALKYIRRGFTIFGYGLVITAVTW
                     LFLPSVYIVFGILHFIGAAILLAPLFARFSPASLILGSVACTIAGFITNAVPGPWPLL
                     WLGIHPASFASLDYVPLLPWFGLVLAGMAFGGLFYPDGRRKFALRTAEPAPARALSFL
                     GRHSLFIYFLHQPLILLLIAIIAPEAMPGLW"
     gene            complement(10809..10967)
                     /locus_tag="PLI76_04730"
     CDS             complement(10809..10967)
                     /locus_tag="PLI76_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61372.1"
                     /translation="MGRICSPFIVLECSRECGFSRIYNEPTGEQSAEIADTKVCPACG
                     APVRRRFF"
     gene            11091..11948
                     /locus_tag="PLI76_04735"
     CDS             11091..11948
                     /locus_tag="PLI76_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2099 family protein"
                     /protein_id="HOI61373.1"
                     /translation="MSDRDEHIVEAAGRCRVVIRNGRVVDVGTPQIDDCPLARRFACP
                     VDEMTPEAIRKNIEGRIRSFGMCTPQREVLAGPDFVIFGASELLSSAVRRGDLDAAVI
                     ASDGAGTLVATNPALIQGIGGRMSGLVKTSPLPAVIARIEENGGAVLDPATAVIDQAA
                     GVALAATLGHRRIAVTTASAAEAAVIRERFSDAVIVAVHTTGMSREDAVLMVRAADLV
                     TACASQHIRDVAAKTALLQAGRSIPVFAMTPAGKAIILGKVAETGQQVVVHGARLPVS
                     GRESPSPLC"
     gene            complement(11953..12648)
                     /locus_tag="PLI76_04740"
     CDS             complement(11953..12648)
                     /locus_tag="PLI76_04740"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOI61374.1"
                     /translation="MQTTSGTRHNTVEAVICDMDNTLFDFVGAKREACRCVVDYIGAG
                     DPEALFSRFISGTYGFEDHRNIRGYLDDLGVYDPGTFEVCCRTYEDVKLDRIEVYPGV
                     EETLRHLADAGIRLAVATDAELVQARRRLGRTGLIDLFEVVVTPEISGKRKPEPDSLL
                     YALRRLDTAPAAAMMVGDSPVRDIAPGRRLGMVTAFAAYGDWHGNGPADAGADIVLAR
                     FSELADHLGIPGR"
     gene            complement(12612..13619)
                     /locus_tag="PLI76_04745"
     CDS             complement(12612..13619)
                     /locus_tag="PLI76_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61375.1"
                     /translation="MNDAIVREAELQVLLNSLDAIRVTYPLYKGDLLVAHPEGTGFRL
                     ELPADRETFQNWLAGYGPIAAELPSYADFSECMFASGISRYTNQAAFDAMRTSYGQLK
                     KTVFFGLDTNLFYHGFASNNPGIDHASYLIVDTVRDEIAYAINRKYPRKMIDEMIEHA
                     PGCREFIGELENKRMKRSRKAAYLALKEYRTIRDRATEIASPGPHTHLSEENDRNIVK
                     ALRKFEEERYALPVLLTADIYMADLCMTEGLEYFYFDRPYRLEATTCTAPAFRRLLFN
                     LAVVFGFIGCNGTTIFGEYGGKGNDLDELKVRFRDDEIYHEFSREVSICRQLLALGIT
                     R"
     gene            complement(13860..14375)
                     /locus_tag="PLI76_04750"
     CDS             complement(13860..14375)
                     /locus_tag="PLI76_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61376.1"
                     /translation="MISAPGDVYITLLGRSTWALVNAYQAVLREKGLRPERVAIVTEE
                     PYAANAAIAAGAVRIVSEGYGFAPTVDVEVLPEADFVQAGRTIRSLASDFIRQGCHVA
                     IDITSGRKVTVAGALIAVSLAELDIRHIYYLAMKSTDDVAKPYMMIPHQIQKIRDIME
                     DAGALSSTASG"
     gene            14695..>15112
                     /locus_tag="PLI76_04755"
     CDS             14695..>15112
                     /locus_tag="PLI76_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOI61377.1"
                     /translation="METRRGKLRTPAETWGLADPEQRRRTRQDAIDDGDLIEITRMGR
                     DIGITFPLAVSARAAQSMVPFPNIPQETVTENLWDTLHAFRDKALMMAAEEFEFQASI
                     YLNGLVPMLTFKAAVSPGDDGEPVITIMLPDEDWETI"
BASE COUNT         2645 a         4702 c         4724 g         3041 t
ORIGIN      
        1 cacccgcgcc ttcggcaaag gatacccgta cacttccgcc ggcacccccg ccctgtccgt
       61 gccttcgtcg gcggactcgc acacttccgc cggcaccccc gccctgcccg cgccttctcg
      121 cggggaccag gagcggtgcc cgggaggcat atcccggtca cccgctacag gcgctatgcg
      181 gccttacgca gtatcttctg cgcaatgttg tagggtgaaa agagatgctc aacctctcgc
      241 gccaggtact ccgtcttccc catgatgtaa tagccgtctg caccgagtgc ggagtgaaag
      301 aggcgtgcaa gttcgttctt ctggttctcg gtgaagtaga tggtgacgtt tctgcaggtg
      361 acggcatcaa ggtaccgggt tatcgggatg cccgacatca ggtcgtgctg ccggaactta
      421 acgagatcct tgatgtggga gcggacctcg aacgtgccgt catcgtggct gacgaagtgc
      481 ttgcggatcc tcctctcatc cacgttctca agcgcctttg cgtcatatat gccggccatg
      541 gccttctgca gcaccacggc gtcgatgtcg gtcgcgtata tcatcacgct gacatccggg
      601 cgaagcgccg tcaattcgtg cgcgaggatg gcgatcgagt agggttcttc acccgaagag
      661 caaccggcac accagatccg gatcgatctc ccgcggccgg caagatccgg caacacctcc
      721 cgccgcacca cctcgaagac ctcgggatcc cggaagaact tcgtgacgtt gatggtgagg
      781 gcgttcctga gcaggttgac ctcctgcggg tttgcaagga ggtacctgtg gtaggcctcg
      841 taatccgcgg cgttcgtcag gcgcatccgc gagaggatgc gccgcttgat gtagtcctcc
      901 ttgtagtttg cacacttgat ctggaccagt ttctcgatgc ttctctggag tgacgcaaat
      961 gcatccatgt gggggctctc ctcctgccgg ggatcgctta tggcatgctc tggtagtgcc
     1021 tgagatcgcc gatcactttc tggacgtcga gccagatgat gagggtcttt gctttcccgt
     1081 cgccgccgct ccccctcttg atgatcgcct tgacatagtt gctgattgac gacgtgatgc
     1141 catcgttgat gcgctcgatc tgatcgtcgc gaaccgagac gacactgtgc acatcgtcga
     1201 cgatgatccc cacgttcgac ccgcccgcca cgtccggcat cagcacgacg atcttctggc
     1261 tctccacccg ttctgaaccc ggaagcccga ggagatctct gagatcgatg atgttcgtga
     1321 tctcgccccg caggttgatg atgcccgcaa ggtaccgggg cgcacggggg aggggagtga
     1381 tcggcatcat ctccacgatc tctcgcgcta tctggatatc cagcgcataa tgctcttccc
     1441 cgagctggaa ttccacaacg tctacggatg ctgccatcgt ctccccctca gttcagcttg
     1501 aactgcttca tgaccttctg gagttgctcg gccatacctg ccagttcgtg cgctgcactc
     1561 ccgacctctt ctgtcgatgc gctgacctct tctgcaagcg ctgccatatc ctcggtgcga
     1621 tccaggttct ccttggtcat gtgggtggac tcgtccatct tctgcatgac gttgttcgtg
     1681 gaacccgcct ggtcttcggt ggccttcgtg atctcggtga tgccgtgggc gacgatttcc
     1741 accccgttga cgatccggct caggcctccg atgaccttgt tgacgctctc gatgcctgcc
     1801 tggatctcct ggtgagatgc cttcatggag cctgcggtct tctcggtgct cgcctggatc
     1861 gtccggatca ggtcctcgat atcctggctc gcccgcttgg attcgccggc aaggttcctg
     1921 atctcgccgg caaccacggc gaacccgcgg ccgtgctccc cggcccgggc ggcctcgatc
     1981 gcggcgttga gcgcgaggag gttggtctgg tttgcaatat cggtgatcaa cttcacgatg
     2041 ttgttgatct cccgcatctg gttgttgagg gtgctgatct cgtccacggt ctgctttgag
     2101 atcccctcga cgaggagcat cttattcgtc gcgacctcgc cgagcgaggc ggcttcatta
     2161 ccctctttcg acgcctttga ggcgtgttcc atgacctctt gcgatgtact tgcaatctcc
     2221 tcgatggatg ctgagagatc gttgatatcc ttcccgatct cttcgatctt gtcgagctgg
     2281 cgccgcgcat cctcagacga ccgctgcgtc gagacggcga cctgctccgt ggcccggatg
     2341 atctcgtcag agctcttgct gacctctctt gttgtcagct cgacctgatg agccgcccgt
     2401 gccacctccg cgaggagcga gcggatggcg gtcagcgagg cgttgtaatc gctcttcacc
     2461 ttctggaggg ggtcgtccgc tcctgcatcc acaaacgcgg tcaggtcgcc cttcgccatc
     2521 gcagcgagtg cagaaccgag cccctcagcg ctttttgcaa gattctcgcc ctcctgtttc
     2581 gctgtctcca tcagggccct gatctggtct tcctcttcct tgctcctggt ccagtcgttc
     2641 cagacgtaga aggccatctc gatatcgcct ttcgcatcga ggatgggtat ggcgttcagg
     2701 gtgaggtatt tcttcacacc gtcgggccac ttgaccatga cgtcggttcg tgcgggtttc
     2761 ttcgtctcgt agcaggcgta gaagtgctcg cccttgagga cggtgatgtc gtagtcgtag
     2821 agtttctttg caagggtctc ctcgcgggtt ccccgccaca tccgggcata ctcgtcgttg
     2881 atgtcgatcc ggctcttatc gggacggagg gtcgcgatcg cgagcgggtt ctgcttgatc
     2941 atcgcatcca cgcggtgctc ggtcttcttg acctcctcct ccttcccctg cagatcggtc
     3001 cagtcgttcc agacgtagaa ggccatctcg atatcgcctt tcgcatcgag gatgggtatg
     3061 gcgttcaggg tgaggtattt cttcacaccg tcgggccact tgaccatgac gtcggttcgt
     3121 gcgggtttct tcgtctcgta gcaggcgtag aagtgctcgc ccttgaggac ggtgatgtcg
     3181 tagtcgtaga gtttctttgc aagggtctcc tcgcgggttc cccgccacat ccgggcatac
     3241 tcgtcgttga tgtcgatccg gctcttatcg ggacggaggg tcgcgatcgc aagagggttc
     3301 tgcttgatca tcgtctcgat gcgatgctgc atcctccgga tctcttcctc ctcttttcgc
     3361 ttctcggtga tatcgttata gacgttcagg atccccgtca gctcgttctt atcgttgagg
     3421 agcgggatcc cgtactgctc aagagtgtag gtgccggcgg ggaactcgac cgtcacttcg
     3481 ccccggaccc gtaccttctc ggtgagcact tttcggatcc cttctccttt ctgggcgaga
     3541 accttgaaat cccgcagcgt catcgaggcg atgcgcgacc ggtcgatgcc ggtcatctgg
     3601 aggtatgcat cgttggccgt gaggatcttc atcgcggtat cgacgaccag gatgggcatc
     3661 gggttcttct ggatgatcgt atcggatcgc tgctgtagtt cggcgatgtt ctccatctgg
     3721 ctcttgatct cttcctctcc ctcccgctgc cgggtgatgt cgttgtagac gatgaggatg
     3781 ctctcgatgg cgccctcctc atcgaggaga gggataccgt actgctcgag cgtatgggtg
     3841 cccgagggaa gttcgacgat gacctcggcg tatgcccgct tcttctcctg gaccgcctgc
     3901 cggaggcctg agcccttctg cgatgttatc ttgaaatctc tcaggctttt gccgagcgcc
     3961 tgacccttcg ggatcccgct catatcggca tacgcggtgt tggcgaccgt gatccggaac
     4021 tgcggatcga cgatcaggat gggcatcggg ttctcctgta tgatcgtctc ggatcgttgt
     4081 cggagggctc ccgcagcctc caggcgggtc cggaggtcct gtttctcctc ctcgctcttt
     4141 gcgagcagcg cgttgattga ggtagccagg ggccggaact cctccggaac gccggcaagg
     4201 tcgatcctcg cggaaccgtc gccgccgatc cccgagcgga gcgcagactc gatcgccccg
     4261 ggcagtacta ccggcccccg ttcctgtcct gtcgtaccgg ggacaaccgg cccggtaaat
     4321 agtgctttcc ttccaggttt atcgatcatc attacacgtc ctttccatag ttcttatcca
     4381 gttccagaag cgccgtggtt attgcccggt attccctgcc ggcatcgctc tcgggcgcgt
     4441 agtgggagat tggcaggcct ttttgctgtg tctcgtagat tgcaggcgag taagggacta
     4501 tgaacacctg tttgaacgct tttctgacct cggcctcaaa catcttcaac cgctctctct
     4561 ccctctcctc ctcgggggag gggccggagg agaagatccg tttcaggaag agggcaaggc
     4621 cgcccgtttt tgccgcccgg tcgtccggcc ctttccagcg ggtcaggatg gccaagtcgg
     4681 tggtgacgtt ctctccgagc atctccctga tgtcgtgaaa gaccgtcgat agggcttcca
     4741 tacccctcag agcaaatgtg ccggggtcga gggtgacgat ggtgtggtcg gcagcgacga
     4801 ggccgttgat gacgaactgg cccatgctcg ggggcgtatc gatcaggatg aagtcgtagt
     4861 ttcccctcac cccggcgagc gcctccctga gcagtcctgc cctgtcctcg atgctgtaga
     4921 ggtagggctc aacccccacg aggtccaggg ttgcaggggc gagatcgact ccggacgccg
     4981 tggggacgat gatatcgttg atcacgacgt cgggaaaacc ctcaaacgcg ctcatgaaga
     5041 gatcgtacat gctcagttca agggtatcgg ggtcgacccc gagccctgcg gtggcgttgg
     5101 cctgcgggtc gcagtcgacc accagaacgc ttcttccact cttctggagg tagcctgcaa
     5161 cgttaaggca cgacgtcgtc tttcccgtgc cacctttgtg gtgggcgaac gctacaattg
     5221 tgatatccag cacccctcat tactttcttg gtaatatgta ttattaaacc tatttctata
     5281 ctacagcgaa tataagagca agacgctgca tttaaagcga tcaacaattc ccttatagga
     5341 aaaccagccc ggatatttcg attgctactc ccgcgggatg acgggggtat cgggcgcccg
     5401 catgcggccg gaggacaggc ggtcagcgga aaccccggcc cggatttttt gtacgcgatc
     5461 ggcgatggac ggccggtagt cgggccccgg gcggaggagg agggggatca cccgcgaaag
     5521 gccgccgtac gttctgcggt gcggggccgg ctcggccgat gcctcaaccc ggagcccggt
     5581 gaggaggttc tggaggagag agccggggtc cttgaccccg tagcagcatc tcatcgcttc
     5641 tgcggcatac cggtcggccg cgaactcctc gtcgcacatg cggcggcagt atatccggta
     5701 gatggagaag acggtcacgg cgaggagagc gatggaggcg aacctgacga gaggaccccc
     5761 tgcctgcagg aaaggcaggg ccgccggcag cagcatgagg gcgaggagga ccgggagggc
     5821 ggagaggaga gaagagccac tcctgatatg cccttcttcg tggagcagca cgaaccgcag
     5881 ggtatcgtcg cttgccccaa agaggtatct ttcgtagaag gcacgtcggt agcggatgca
     5941 gttgagcgcc gggcagcctc gcgcagaagg cgtgaagggg cactgcacca gccttctctc
     6001 gcggccgatc agccccttcc cctggagctc gcggaatatc cggtggaggc gggagtcatc
     6061 gtcggcctta tccattcaag atatattata ttttgtacac tatattaaat gatttcacat
     6121 cccgcagaac cgttcatcat ccgcctcgcc tcttttccgg gctatcgagc gctcaaggca
     6181 gccggggcca cgccgtcgcg cccgaccggt ggggatgccc ccggctccgg cccggagcgg
     6241 caccggatcg ggaacctgtg caatgtccgt ttttttcata atgttttaat aatagcaacc
     6301 aaaatggtga atgagtagag tggaggtgaa tttgcagcac cgggggtctg tacggcccgc
     6361 accgcgtggg cgcggcgtct gtccccccgg ttctccgagc gaatgtccag atacgtgggt
     6421 ttcctgatac ttgcccttgc cgccctcacc gtggctgccg ggtgcctctc cacaggattc
     6481 ggggaggtga cctacggcgg cgacgccctg caggtccgcg tggagaacac cggcaggccc
     6541 gtcgagaatg ccgtgctcca ggtgacggtc actgaggttg gcgcgcttga acagcacgag
     6601 atcctctctg aagcacggta tgtcaacctt gccccgggag agaacgagta ctcggtcccg
     6661 gtcaacctgc agcccggtac ttacaggctc tttttgacca tattcatcga cggcgagcgg
     6721 aaagcatccg tcatccgtga cctggaggtc gcaccctgac accggcaggc cttgccgcgg
     6781 cgcgggggct tgagccggca gacgtcgtct tctccggcgg cgaggtcttc aatcccttcg
     6841 cgtgcgcctg ggaacgcacg gacgtcgcgg tcagcggcgg ccgcatcgtc ggtctcggcg
     6901 actacgaggg gaagcgggag tacgacctca cgggaaccta tgttgttccc ggcctgatcg
     6961 atgcccacgt ccacatcgag agttctctcc tcgcgccggc ggagtatgcc cgactcgtcc
     7021 ttcgatgcgg cacgacgacc gtgatcgccg acccccacga gatagcgaac gtctgcgggg
     7081 cgccggggat cgagtacatg ctcgccgaag gggcgcagac accgctcgac attctggtga
     7141 tgctcccctc atgcgtcccg gcaacgccct tcgacctggg aggcgcccgg cttgccgccg
     7201 cagacctcgc ccggttccgg gggcgggaag gggtcgtcgg gcttgccgag gtgatgaacg
     7261 tccccggcgt ccttgccggg gatgaggatc tccgggcaaa gatggatctc tttcccgtca
     7321 tcgacggcca cgcgccgctc ctctccggga aggacttaaa cgcctacatc tacgccggcg
     7381 tccagagcga ccacgagtgc acggcgctcg cggaggcgcg ggagaaactc ctcaggggga
     7441 tgtacgtcat gatccgggag ggctcgacgg agcagaacct cgccgatctc ctgccgctcg
     7501 tggatgcctg cacggcgccg cgatgctgct ttgccaccga cgaccggcac gtcgatatgc
     7561 ttgccggaaa ggggcatatc gacgactgca tccggagagc cgtcgcgtgc gggcttgagg
     7621 tcgagcttgc gctccgcatg gcgacgctct ccgccgccga gcggttcggg ctccacgacc
     7681 ggggcgctct tgctcccggg aggcttgccg acttctgcat cgtcgacgac cccgaccggt
     7741 tccgcgtcct gcggacgttc aagcgcggca tcgaggtggc cgatgccggc taccgcccgc
     7801 cggcctgccc tgcggccccc ctgcatgccc gcgtgccggc cccgcgcgat atccggatcg
     7861 ccggccgggg cgaggcgcgg gtgatcggta tcgtgcccgg ccagatcgtg acccgtgacc
     7921 tgcgctgccc ggtggatgcc gccgatatcc cggacaccga ccgcgacatc ctcaaagtgg
     7981 tcgtcaccga ccgctaccgg gggggaggct ccggcgtcgg gctcgtccac ggtctcggtc
     8041 tccgggaagg ggcgcttgcc ggctcggtct cccacgactc ccacaacatc gtcgctgtcg
     8101 gggtcgccga cgccgatatc gtccgcgcca tcggcgaggt ggtccggctc gggggcgggc
     8161 ttgcggcggt cttgggcaac gaggtgaccg cgctccccct cgagtgcgcc gggctgatgt
     8221 cggcccttcc ctacgatgaa gtcgcgcgaa gcctcgcggc cctcgaagag cacgcccggc
     8281 ggctcggcgc ggtcgagaac cccttcatgt acctctcgtt cctcgccctg accgtcatcc
     8341 ccgccacccg ggtcacggag cggggggtct ttgacgttac ggcgtttaag gacgtgccgc
     8401 tgttttcgga gtgaaaaagg gcaggagatc gcctggcggt gcttgctctc tccccgatcg
     8461 cccctttcga gttagatccg cttcgtgccc ggcgcgaaaa aagggggaga ccaccccggc
     8521 ggcgggtcac tccttgcctt tcttcacttc tgcgatctct tttgatatgg aacgggcagt
     8581 ctctgccatg ggagccgggg cgtcgtctgc gcggaggctc ctgagggccg ggcaggcatc
     8641 ggcctgcacg ctcactatct ccggacaggt tccggtgcgc cggttcctga taagggatgc
     8701 gatgatggtc acccccagga tcacgaggat gatagcgagc gataccacca ccgggacatg
     8761 gtagatctcg acgaccagca tcttgacgcc gatgaaactg aggatgccgg cgagcccgta
     8821 cttcaggtag cagaacgcgc tcatgatgtg cgccagcgcg aagtacatcg acctcaggcc
     8881 gaggatggcg aaggcgttgg agctgaagac gatgaacggg ttggtggtga tgccgaggat
     8941 ggcggggatg gagtccaccg cgaagaggat gtcggtggtc tccaccacca gcagggtcac
     9001 gagcatgggg gtggcccaga gcaccgccct gccggccgca tcagtcttcc tgacgaagaa
     9061 cctgtcccct tcgtagttcc cggtgaccgg catgatcttc cggaacccgc ggaccaggat
     9121 gttcttgtcc ggttcgaccg ctttctcctc ctttttggtc accatcctga taccggtgaa
     9181 gatcaggaag gcgccgaaga tgtagagcac ccaggagaac gtctccacca gcgcgacacc
     9241 cgccatgatg aacgcaaaac ggaacagcag ggctcccagg atgccgtaga acagcacctt
     9301 gtgctggtcc tcctgcggca cgcagaacga ggcgaagacg atgacgaaga cgaagagatt
     9361 gtcgacgctc agcatcagct ccatgatgta gccggtggtg aactccagcc cggcctcctg
     9421 ccccatccag gtgtagatgc cgatattaaa gagtactgcg gcggcgatga agacggcgac
     9481 ctgcaggagc gcttccctcg gctttatgac gtgggcttta cggttgaaga cgccgaggtc
     9541 taacgccagg agcaccacga tactgatgac gaacaggatc cacgctacgt ctgtcacttc
     9601 tgtcatagat tcacccggct cgatctgtca tatttcgggc tttccgggca cacattaccc
     9661 gggggccctc cgggcatcgt ctcgaccgta tcgggatgcc ggtacgcggg gcggaagaag
     9721 aactcaggat aggttttact ccggacgacg tcatcggtgt ttgggccgca cctgccgcaa
     9781 ccggcatgcc cctctccgga ataccctcaa gacagctctg catatgcgga tcggatcgcc
     9841 gggacgcccc ctccccgaca gaagcgcgtt catcggggtc cggtccggca acgtcccggc
     9901 tctttaataa ccggtcaaac ccggcctctc cccagatcat ttcagttccc ccggcaaata
     9961 ctttctgccg gcgataaaac aacctcctgt cagggagcgg agaggctccg ctgtacccct
    10021 gtgatgcacg tgcggttcgc gaacccctca ccacagcccc ggcatcgcct ccggcgcgat
    10081 gatcgcgatc aggaggagta tgagaggctg gtgcaggaag tagatgaaga gcgagtgccg
    10141 gccgaggaac gagagcgccc gggccggtgc cggctctgcc gtccggagcg caaactttcg
    10201 ccgcccgtcc gggtaaaaaa gccccccgaa ggccatgccg gcgaggacga ggccgaacca
    10261 ggggaggagc ggcacgtaat cgaggcttgc gaaggacgcc gggtggatgc cgagccagag
    10321 gaggggccat gggcccggga cggcgttcgt gatgaagccg gctatggtgc aggcgacgct
    10381 cccgagtatg agggacgctg ggctgaaccg cgcaaagagc ggcgcgagca ggatcgcggc
    10441 cccgatgaag tgcaggatgc cgaagacgat gtagacggag gggaggaaga gccaggtgac
    10501 ggcggtgatg acgaggccgt agccgaatat cgtgaatccg cgccggatat acttgagcgc
    10561 aaggtcgcgg ccggcgaggc gctgcgaagc tctggcatag ctgatggtga aggagacccc
    10621 gacgatgaag atgaacgtcg acgcggtcat gtaggcgagc atgcggagga acccgccgga
    10681 gacggggagc ggcaggacgc cgaagaagtt gaggtcgaag gcggagtgaa agacgatcat
    10741 cgtcacgacg gcgatgcccc gggcggcgtc gatctcccag tagcgctctc cctgcacggt
    10801 ttctgccgtc agaagaacct tctccgcacg ggggcgccgc aggcaggaca gaccttcgta
    10861 tcggcaatct cggcgctctg ctccccggtg ggttcgttgt agatccggga gaaaccgcac
    10921 tctctgctgc actcgagcac gatgaacggg ctacagatcc ggcccatgcc tcctcacccc
    10981 gtcttggcgc accatggtct acattctcat ggagagatca ccggataagt agttttaccc
    11041 gctggcccgg gagggagagc atttttcccc atccacgccc taccggaggt atgagcgacc
    11101 gtgacgaaca tatcgtagag gccgccggca ggtgccgggt cgtcatccgg aacggccgtg
    11161 tggtcgatgt cgggacgccg cagatagacg actgcccgct ggcacggcgg ttcgcctgcc
    11221 cggtcgatga gatgacgccg gaggcgatac ggaagaacat cgaagggcgc atccggtcct
    11281 tcggtatgtg tacgccacag cgcgaagtcc tcgccgggcc ggacttcgtc atcttcgggg
    11341 cctccgaact cttaagcagc gccgtccgga ggggagacct cgacgccgcg gtgatcgcct
    11401 ccgatggggc cgggacgctg gtcgcgacga accccgccct catccagggt atcggcggcc
    11461 ggatgtcggg cctcgtgaag acgagccccc tccccgcggt gatcgcgcgt atcgaggaga
    11521 acggcggtgc ggtcctcgac ccggcaaccg ccgtcatcga tcaggccgcc ggcgtcgccc
    11581 tggcggcaac gctcggccac cgccggatag ccgtgaccac cgcctccgcg gccgaggcgg
    11641 cggtcatccg ggagcggttc tcggatgccg tgatcgtcgc cgtccacacg accggcatgt
    11701 cgcgggaaga cgcggtcctg atggtccgcg cggccgatct ggtcaccgcc tgcgcttcgc
    11761 agcatatccg ggatgtagcg gcaaagaccg cgctcctgca ggcaggaagg tcgataccgg
    11821 tctttgcgat gaccccggcg ggaaaggcga tcatactcgg gaaggtcgcc gagaccggcc
    11881 agcaggtcgt cgtccatggc gcgcgcctcc cggtatcggg tcgggagtcc ccctcgccgc
    11941 tctgctgacg cttcaccggc ccgggatgcc gaggtgatcc gcgagctcgg agaagcgcgc
    12001 aagcacgata tcggccccgg catccgccgg gccgttcccg tgccagtccc cgtaggcggc
    12061 aaacgcggtc accataccaa gccgtctccc cggggcgatg tcgcgaaccg ggctatcgcc
    12121 caccatcatc gccgctgccg gcgccgtatc gagccgccgg agggcataga ggagggagtc
    12181 gggctcaggc ttgcgtttcc ccgatatctc cggggtcacc acgacctcga agagatcgat
    12241 aagcccggtc ctcccgagcc tccggcgcgc ctggacgagc tctgcgtcgg tggcgaccgc
    12301 gagcctgatg ccggcatccg cgaggtgccg gagcgtctcc tcgacgcccg ggtagacctc
    12361 gatcctatcg agtttcacgt cctcgtaggt gcggcagcag acctcgaacg tccccggatc
    12421 gtacaccccg aggtcgtcaa ggtagccgcg tatgttcctg tggtcctcaa acccgtaggt
    12481 gccgctgata aaccgcgaga agagcgcttc agggtctcct gccccgatgt agtcgaccac
    12541 gcaccggcac gcctcccgct ttgctccgac gaagtcaaag agggtgttgt ccatatcgca
    12601 gatgaccgcc tctaccgtgt tatgccgagt gccagaagtt gtctgcatat cgagacctct
    12661 cgtgagaatt catggtagat ttcgtcgtcc cggaaacgca ccttcagttc gtcgagatcg
    12721 ttgcccttcc cgccgtactc cccgaagatc gtggtgccgt tgcagcctat gaacccgaag
    12781 accacggcga ggttgaagag cagccgccgg aatgccggcg ccgtgcaggt tgttgcctca
    12841 aggcggtatg ggcggtcgaa gtagaaatac tcaagccctt ccgtcatgca gaggtccgcc
    12901 atgtagatgt cggcggtcag gagaaccgga agggcatacc gctcctcctc gaacttccgc
    12961 agcgccttga cgatgttacg gtcgttctcc tctgagaggt gcgtgtgcgg gcccggcgag
    13021 gctatctccg tcgcgcggtc gcggatggtg cggtactctt tcagggcgag gtatgccgct
    13081 ttccgggacc gtttcatccg cttgttctca agctccccga tgaactcccg gcagccgggc
    13141 gcatgctcta tcatctcatc gatcatcttt cgcgggtact tgcggttgat ggcatacgcg
    13201 atctcgtcgc ggacggtatc gacgatcagg taggaggcat gatcgatccc ggggttgttc
    13261 gatgcgaaac cgtggtagaa gaggtttgta tccaggccga agaagaccgt cttcttcagc
    13321 tgcccgtacg aggtgcgcat cgcatcaaag gcggcctggt ttgtgtaccg gctgatcccg
    13381 cttgcaaaca tgcactccga gaagtcggcg taagaaggga gctcggcggc gatcggcccg
    13441 tatccggcga gccagttctg gaaggtctcc ctatcggcgg ggagttcaag tcgaaaaccg
    13501 gtgccttccg ggtgcgcgac gaggaggtct cctttgtaga gcgggtacgt gacccggata
    13561 gcatcgaggc tgttgaggag gacctgcaac tcggcctccc tgacgatcgc gtcgttcacg
    13621 gtatcacggc gcgggtgcct ccggtaaata tcggaggagg aagcgcgccc tccttaccag
    13681 cccaaaggtg atcccgggta caacgcatcg gcgctcccgc gggcgaggca tgcacggcgg
    13741 gcaagcccgt cgccggataa ccggcaccgg ccggccggtg cgaataaggc cgcctgtaca
    13801 cattccatgg aaacgtctcc ggcgcactca aaataccttt ccctgcagcc acccggggat
    13861 caaccggagg cggtcgacga gagcgccccc gcatcctcca tgatatcacg gatcttctgg
    13921 atctggtgcg ggatcatcat atacggcttt gcgacgtcgt cggtgctctt catggcaagg
    13981 tagtagatgt gccggatgtc aagctccgca agcgagaccg ctatgagcgc cccggcgacc
    14041 gtgaccttcc ttcccgaggt gatatcgatc gccacgtggc atccctgccg gatgaagtcc
    14101 gaggcaagtg aacggatcgt ccggccggcc tgcacgaagt ccgcttcggg cagaacctcc
    14161 acatcgaccg tcggggcaaa cccgtatccc tccgagacta tacggactgc cccggccgcg
    14221 atcgctgcgt tcgccgcgta aggttcttcg gtgacgatag cgacccgttc ggggcgcagc
    14281 cccttctccc gcaggactgc ctggtatgcg ttgacgagcg cccatgtcga gcggccgagg
    14341 agtgttatgt agacatcgcc gggggcagag atcatcgggg gatcaccgga gtagactcac
    14401 cccgcttgct atataggctt tgtcgggagc cccctttcgg gatgaggtgg tgcggtgggc
    14461 attccaggcc gcacgctccg tcctttttgc ctccttcccc gtatatccgc ctctcccggg
    14521 gccatgcccg gtacatcgcc tgatttttgc ggcattccgc tcccgggatg ctcccaatac
    14581 gcctttaacg ctaaaggaat tgcaaaatct cgcaaataaa ttggtaaatt ttttatgacg
    14641 ggttccctca tagtggtatg gtagagcgcg aggatatccc ggagaatacg atccatggag
    14701 acgagaagag ggaagttgcg gacgccggca gagacctggg gattggcgga cccggagcaa
    14761 cggaggagga cacgccagga tgctatcgat gacggggatc tgatcgagat cacccggatg
    14821 ggcagggata tcggcatcac cttccccctc gcagtctcgg cacgggctgc gcagagcatg
    14881 gtcccgttcc cgaacatccc gcaggagacc gtgacggaga acctgtggga caccctccat
    14941 gcgttcaggg ataaagccct tatgatggcc gcagaggagt ttgagttcca ggcgagcatc
    15001 tacctcaacg gcctcgtccc catgctcacc ttcaaagccg cggtctcccc cggggatgac
    15061 ggcgaaccgg tcatcaccat catgctgccg gatgaggact gggagacgat cg
//
LOCUS       DAOSEE010000042        15098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1575_length_15098_cov_254.081633, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000042 DAOSEE010000000
VERSION     DAOSEE010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 15098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15098
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1575_length_15098_cov_254.081633"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PLI76_04760"
     CDS             <1..711
                     /locus_tag="PLI76_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HOI61378.1"
                     /translation="LSESFDYTKEALWGRWVRWLLLLVSVVIFPLIYGYSLRVMSGTK
                     PAPELEGWIGLFVDGIKLIVITIVYAIPVWVLAIVGLAAYFIPVSTTTTPGVCLATQQ
                     CIRCAYFIPVSTTTIPGSGPETGIFVLLALLIVFIIFAVVIGILSTFAMVRFARTGRM
                     GEAFRVRALVAHIGEIGWLNVFIALVVLTVVLAIVELVLVLVPLIGVPLLLILIPAFI
                     IFVYRYIALIYESAPAPA"
     gene            792..2303
                     /locus_tag="PLI76_04765"
     CDS             792..2303
                     /locus_tag="PLI76_04765"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOI61379.1"
                     /translation="MIVLLAESIRFFDTTLRDGEQTPGVSLTPAGKLEIATHLADIGV
                     HVIEAGSAAASAGERESIRVIAEAGLAAECCTYVRALAGDIDLAADAGADSVHLVVPV
                     SDLHISAKLRKTREQVCDMAWSAVEYAKERGLIVELSGEDASRADQAFLAEVFREGVE
                     RGADRLCFCDTVGLLTPERAAAIIPPLLSAPLSIHCHDDLGFGLANTVAALRAGATCA
                     HVTVNGLGERAGNTALEELVMSLETLYGVDTGIRTEELYPLSTHVARLTGVPLATNKP
                     IVGEMAFTHESGIHAHGVMRNASTYEPLRPERVGRRRRIVIGKHSGSTAVEAALNDMG
                     YAPDSAQLAEIVARIKQIGDAGMRVTDADIMAIADTVMEIEFTPCLKLRQFTIVSGSN
                     AVPTASVTMLVNGGEVTGAAVGTGPVDAAIRALQKSVANVGSVRLDEYRVDAITGGTD
                     AVVDVSVKLSRNGKTVTSRGARTDIIMASVEAVIAGMNRLLSEEHEDRSQDSD"
     gene            2275..3948
                     /gene="ilvB"
                     /locus_tag="PLI76_04770"
     CDS             2275..3948
                     /gene="ilvB"
                     /locus_tag="PLI76_04770"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOI61380.1"
                     /translation="MKTGARTLIEALQREGVDTIFGYPGGVVLPIYDELYDSSIRHIL
                     VRHEQAAAHAADGYARASGRVGVCLATSGPGACNLVTGIATAYMDSIPIVALTGQVPT
                     GLLGNDAFQESDITGITMPVTKHNYLVKDAGDLDRTIQEAFYIARTGRPGPVLIDLPK
                     DVTTGEVKGGGAAPGEVCLRGYQPTYQGHVRQIDKALDLVVQAERPLIYAGGGVVLSG
                     ASAELLEFAETAAIPVTTTLMGLGAVPGDHPLSLGMLGMHGTQSANYAVTECDLLLAV
                     GVRFDDRVTGRIGTFAPNAAVIHIDIDPAEIGKNKPVDVPIVGDVKAVLQALLRRMQK
                     RGDTTNWVARTNAWKAQYPLTYREDDHLRPQYVIRELSDILKGEAIITSEVGQNQMWT
                     ALYYCFKKPRTWITSGGLGTMGYGFPAAIGAHFARPDVPVVDVAGDGSFQMNIQELGT
                     VAHYRIPVKVMILNNMYLGMVRQWQELFYDRRYSYTELPPVDFVKIANAYGVDGIRVD
                     AKDGVREAIETALATDGPFVLDFRVEREENVFPMVPAGAAINEMIGVRQ"
     gene            3945..4448
                     /gene="ilvN"
                     /locus_tag="PLI76_04775"
     CDS             3945..4448
                     /gene="ilvN"
                     /locus_tag="PLI76_04775"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOI61381.1"
                     /translation="MKLHTLSVLVENRAGVLSRVAGMFSRRGFNIESLAVGTCEEPNM
                     SRITIVVNGDDGVVEQVMKQTNKLIDVIKVSDLTERESVERELALIKVATETGPARAE
                     VMQIADIFRAQVVDVGAKTLVLQVTGDTGKIDALESLLRQYGIKELVRTGKIAILRGV
                     KTVKSSK"
     gene            4656..6194
                     /locus_tag="PLI76_04780"
     CDS             4656..6194
                     /locus_tag="PLI76_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOI61382.1"
                     /translation="MADLTVLAVIGLYFIVLIGIGSWASKKIHTTEDYILAGRSLGFW
                     VFTILIVCSICSGMTLLGVSGFGYTSGWPGIWEQIFVPLAASFCIIVFGVKLNAVGKE
                     RGYLTIQDYLAERFESPRALRGLSAVSGIVVSLIYLVGQYAAITIVLVWLFGIPHWQA
                     LLISGVIITAYTVVGGLYAVSWTTLFQGGILILGVLLMAPPVIMSAGGLTHINTVLAG
                     VDPNFVGPWFPEAYAPYAYATPEFLFSFAILLMVGLACAPHVVNNVLAAREARYFKWA
                     PLVAFLIYAVVMLLVKLVGFAVRSLVEEGRLVLPDAVNAQDYSFIVGVEHAMPNVAFW
                     AFFAVIVLAAVMSTTDRLMLTVGTSFAWDIYKNILRPSASDREVLLVSKIALVLGAGG
                     TLLLAINPPEMLAWLIWMGIGVMLATFAVPLLAGLYWRGATAGGAIASMVVGLVAAAA
                     FGYWHQFVAPLPVHFSLFALAFSALAMVVASRLSTRNSEETLANTRTGWFIQSPGHDA
                     PRRH"
     gene            6500..8083
                     /locus_tag="PLI76_04785"
     CDS             6500..8083
                     /locus_tag="PLI76_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOI61383.1"
                     /translation="MADIVTLALVGLYFVVLIGIGSWASKKIHNTEDYIVAGRSLGFW
                     VFTILIVCSICSGMTLLGVSGFGYTSGWPGIWEQIFVPLAASFCIIVFGVKLNAVGKE
                     RGYLTIQDYLAERFESPRALRGLSAVSGIVVSLIYLVGQYAAITIVLVWLFGIPHWQA
                     LLISGVIITAYTVVGGLYAVSWTTLFQGGILILGVLLMAPPVIMSAGGLDHINTVLAG
                     VDPNFVEPWFPAAYAPYAYATPEFLVSFGILLMVGLACAPHVINNVLAAKEARYFKWA
                     PLVAFAIYAVVMFLVKFTGFAVRSLATEGAITLPAAVNAQDYSFIVGVEYAMPSVLLW
                     AFFAVIVLAAVMSTTDRLMLTVGTSFAWDIYKNIFRPSASDDEVLLVSKVAVVLGAGG
                     TLLLAINPPEMLAWLIWMGIGVMLATFAVPLLAGLYWRGATKEGAIASMAVGLIAAGV
                     FGYWSKFVGSLPVHFSFYALLLSALAMIAVSLLTARISTDTLDNTRTGWFIQSPGQPS
                     RAGSKGDAWLVEQDTPRQH"
     gene            8275..8766
                     /locus_tag="PLI76_04790"
     CDS             8275..8766
                     /locus_tag="PLI76_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328087.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HOI61384.1"
                     /translation="MRIVIYYFTGTGNSLAAAKKIAAVLGDCEIVPIASLANTPGAIA
                     PEAGRIGIVCPVYFSGLPAMVTAFAKRLDTAPAGYLFAVVMHGGGGGAAVCPAGNIDM
                     VDGRPVWNHRCELCCGCIHLCPAGAIQAGSRTEGRQRYRHPDVTVADMERQAGRNRTR
                     RAG"
     gene            9009..10397
                     /locus_tag="PLI76_04795"
     CDS             9009..10397
                     /locus_tag="PLI76_04795"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOI61385.1"
                     /translation="MQISVISGSPKGEKSVTFQYVRFLEQAFPDHTFTVVHAGRDIRA
                     IEEREEVWRGLLATVEGCEGVLWATPVYVMLVPAQLKRFIELVGERNAAEAFSGKYAA
                     CLTTSIRFFDHTAHAYLHGVCDDLGMLYTGFCSAHMEDLRNEAFQEQFILFFSDFLEA
                     IIEQRPVQRVFPPLPAASACCALKTSPAAVDTRGRRVVILHDAEPGSGQEAMVRHLAA
                     CYAGPVQVAHVRDAGMKGGCLGCCRCAFENTCIYDDGFRAFWNEYVVPADILVMAGTV
                     RDRFFSAAWKQFFDRSFSNGHVPAFEGKQIAYLVEGPLGHLPNLREVLTGLATMEGAN
                     LAGIVTDESDRIDAALHTLAERSVRLSERGYIAPATFPAVAGHKIFRDEIWGGMRAIF
                     KADDRYYRQHGLYDFPTRDYPKRIVTRATSLAMSIPAFRRDVVEKMPAYMVRPLEKAI
                     ETSTVLAKRRAK"
     gene            10568..11881
                     /locus_tag="PLI76_04800"
     CDS             10568..11881
                     /locus_tag="PLI76_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOI61386.1"
                     /translation="MKTAVLGGGLTGVTLARLLHERGDDVTVLERSETIGGLCRSKTE
                     AGFTFDIGGSHIIFSRDAEALSFMLSALGGNADRRKRNTKILYKGRYVKYPFENGLMD
                     LPAEDRFFCINEFVRNLIAVEKGEVPPPANFAEWIVSTFGRGIAECYMLPYNEKIWNY
                     PADRMSHHWVDGRIPRPPIEDIIRSAIGIETEGYTHQAVFSYPVEGGIEALVRAIAEP
                     VLPAVRTGFTVSSIREEGDGFAVSDGRETVHAGRLISTIPLQNLLPCLSGVPADVRAA
                     CDALRYNSLCSVFIGIAGEVPDISWLYVPDEAAGLFNRVSFPSNYSTRVAPAGHSSIL
                     AEITYNDGDAVSRMADADLIEHTVTSLVAAGLIPSRDTVVYTGIARQEFAYVVYDIDY
                     QKNIGIVREFCREKGIDLVGRFSRFEYLNMDGCIRSAIDFLRGDG"
     gene            11878..12855
                     /locus_tag="PLI76_04805"
     CDS             11878..12855
                     /locus_tag="PLI76_04805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOI61387.1"
                     /translation="MKVNIFVEDFRFLRYIGCATAARTLYNHLARLPDMEVSRNLYHG
                     DFDLTHYHTFGPWAMYHLKFTEGVKVLTAHSTPRLNEGNVAFSKRINTIYPKIYRQFD
                     HIITISEPCRRETEQLVPDVPKTLIPNGVDRAYFKRDKKKRKSFRDEFEIEPGQRVVL
                     SVGQQTPRKGIYDFFRLADQHPDMTWVWVGGFPYGTLSKDYAKIQMLKTHAHANVIFT
                     GIIDDISRAYSGADVFFMPSHAETFGLVIVEALSAGLPVIARDIFEFKEIFGDSVLFF
                     GDLDDARVLVNNDAALRRCASRARASTENYDITLIAQRHQQLYRELIEA"
     gene            12852..13550
                     /locus_tag="PLI76_04810"
     CDS             12852..13550
                     /locus_tag="PLI76_04810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOI61388.1"
                     /translation="MISVVVPTYNEEQNIERCLASLADQTVPRETYEIIVVDGNSRDR
                     TRELAEPLADKVFIQTSKRVGGARNDGAMAASGDIIATTDADCILPRDWVERIGQNFA
                     ERDIVQLYGTVYPIEGGLKNHLSLLGANTFARLGCYTRTIYFTLGCNTAFDREAFIRA
                     GMYRCIDAGDDLEIAQRMRKLGRVRLDTRLKVGFSMRRYQQFGTLKSVWEWLYIVLSG
                     GDAGGATYSQREYK"
     gene            13555..13974
                     /locus_tag="PLI76_04815"
     CDS             13555..13974
                     /locus_tag="PLI76_04815"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOI61389.1"
                     /translation="MERTPSIPEAYARVVQRSDACEFARLLGMAVTEIEGGRVRVAMP
                     AEGTKNVHGTTHGGAIFSLADHAFGIAANMEGIDQIAISARIRYFTVPAADTLEAIAR
                     RVSETERTSVYAVDVFSGDRLVASFEGVGFKIGTVPE"
     gene            complement(13985..14476)
                     /gene="msrA"
                     /locus_tag="PLI76_04820"
     CDS             complement(13985..14476)
                     /gene="msrA"
                     /locus_tag="PLI76_04820"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HOI61390.1"
                     /translation="MAQEKKIERATFGAGCFWGIEETFREVRGVVDTAVGFMGGSTEN
                     PTYPDVCTGKTGHTEVVQVTYDPGEVSYRDLLNVFWNAHDPTTLNRQGPDIGTQYRSV
                     IFYHTTEQEAEARASRAAVEQSGRFRRHVVTAIEPAGTFWRAEEYHQQYFAKHGGGHC
                     RVM"
     gene            14567..>15098
                     /locus_tag="PLI76_04825"
     CDS             14567..>15098
                     /locus_tag="PLI76_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HOI61391.1"
                     /translation="MIFESDTVEMVARLMALSARTAPKARGIDVIRTLIVTGDERRVL
                     ARAMREYGERHNAGFFIRDAGNVEASDACLIIGSLLADAVGLDCGGCGYATCAEMLKA
                     QGNLRSTPFCGPNCVVRMADIGIAVGSAVKTASIHNVDNRVMYTVGVGALSLGWLEGC
                     GVAYGIPLRASGKDIFF"
BASE COUNT         2646 a         4920 c         4673 g         2859 t
ORIGIN      
        1 ctcagcgaat ccttcgacta cacaaaggag gccctctggg gcaggtgggt ccgctggctc
       61 ctcctgctcg tcagcgtggt catattcccg ctcatctacg gctacagcct gcgggtcatg
      121 agcggcacga agcccgcccc cgaactcgaa ggctggatcg ggctcttcgt cgacggcatc
      181 aaactgatcg tcatcacgat cgtctacgca atcccggtct gggtcctcgc gatcgtaggc
      241 cttgcggcat acttcatacc ggtctcgacg acaacgaccc cgggtgtctg cctggcaaca
      301 caacagtgta tccgttgcgc atacttcatc ccggtctcga cgacaacgat cccggggtcc
      361 ggccccgaga cgggcatctt cgtactcctc gccctcctca tcgtcttcat catctttgcc
      421 gtcgtcatcg gcatcctctc gacctttgcg atggtccggt ttgcccggac ggggaggatg
      481 ggcgaagcct tcagggtccg tgccctcgtt gcccacatcg gggagatcgg gtggctgaac
      541 gtcttcattg ccctggtcgt gctgaccgtc gtgctcgcga tcgtggagct cgtcctcgtg
      601 ctcgtgccgc tcatcggtgt gccgctcctg ctcatcctta taccggcgtt catcatcttt
      661 gtctaccgct acatcgccct catctacgag agcgccccgg cgccggcatg aaccctcctt
      721 ttttccgccc tgactgccca atatttaaat agcctcaccg ccaaccattc ttccggtgaa
      781 acccactatg agtgattgtt ttattagctg aatcgatccg cttttttgac actaccttac
      841 gggacggcga acagacaccg ggtgtctcgc tcacgccggc cgggaaactt gagatcgcaa
      901 cgcacctcgc agatatcggc gtccacgtca tagaagccgg ctctgccgct gcatccgcag
      961 gagagcgcga gtccatccgc gtaatcgccg aagccgggct tgccgccgag tgctgcacct
     1021 acgtccgggc tcttgccggg gatatcgacc tcgccgcaga cgccggcgcc gactccgtcc
     1081 acctcgtcgt cccggtaagc gatctccaca tcagcgcgaa gctcagaaag acccgcgagc
     1141 aggtctgtga catggcctgg agcgccgtcg agtacgcgaa agaacgcggc ctgatcgtgg
     1201 aattatccgg cgaagacgca tcccgcgccg accaggcatt cctcgcagag gtattccgcg
     1261 aaggagtcga gcggggggcc gaccggctct gcttctgcga caccgtcggg ctcctcacgc
     1321 ctgagcgggc ggccgcgatc ataccgccgc tcctctctgc cccgctctcc atccactgcc
     1381 acgacgacct cgggtttggg ctcgcaaaca ccgtcgccgc cctccgcgcc ggggcaacct
     1441 gtgcccacgt caccgtcaac ggcctcgggg agcgggcggg gaacacggcc ctcgaagagc
     1501 tggtgatgtc gcttgagacc ctctacgggg tcgataccgg catccggaca gaagaactct
     1561 atccgctctc gacccacgtc gcccggctga ccggggtgcc gcttgcgacc aacaaaccca
     1621 tcgtcggcga gatggccttc acccacgaga gcggcatcca cgcccacggg gtgatgcgga
     1681 atgcaagcac ctacgaaccc ctccggcccg agcgggtggg aaggaggcgc cggatcgtca
     1741 tcgggaaaca ctccgggtcg acggcggtcg aggccgccct caacgacatg gggtatgccc
     1801 ccgacagtgc gcagctcgcg gagatcgtcg cccggatcaa gcagatcggg gatgccggga
     1861 tgcgggtgac cgacgccgac atcatggcga tcgccgacac cgtcatggag atcgagttta
     1921 cgccctgcct caaactgcgc cagttcacca tcgtctcggg gagcaacgcg gtcccgaccg
     1981 cctcggtgac gatgctcgtc aacggcggcg aggtcaccgg tgccgcagtc gggaccggac
     2041 cggtggatgc ggcaatccgc gcgctccaga aatcggttgc gaacgtcggc agcgtccggc
     2101 tcgatgaata tcgcgtcgat gcgataacgg gaggcacaga tgccgtcgtt gacgtatcgg
     2161 tgaaactcag ccggaacggg aagaccgtga catcacgggg tgcccgaacc gacatcatca
     2221 tggcaagcgt cgaagcagtt atcgccggga tgaacagact actcagtgaa gagcatgaag
     2281 accggagcca ggactctgat tgaagcgctg cagcgtgaag gggtagatac catattcggc
     2341 taccccggcg gcgtggtgtt gccgatctac gatgaactct acgactcgtc gatccggcat
     2401 attctggtaa ggcatgagca ggcagcggcg cacgcggccg atggctacgc gcgcgcaagc
     2461 ggccgcgtag gggtatgcct tgccacatcc ggtcccggcg catgcaacct ggtcacgggg
     2521 atcgcgacgg cgtacatgga ctccataccg atcgtggcgc tcaccggcca ggtgccgacc
     2581 ggcctcctcg ggaacgatgc attccaggag tcggacatca ccggcatcac gatgccggtc
     2641 accaaacaca actacctggt gaaagatgcc ggcgacctcg accgcaccat ccaggaagcg
     2701 ttctacatcg cgcggaccgg ccggcccggc ccggtgctga tcgacctccc caaagacgtc
     2761 accacgggcg aggtaaaggg cggcggggcc gcacccggtg aggtctgcct ccggggctac
     2821 cagcccacct accaggggca cgtccgccag atcgataaag cgctcgatct ggtcgtccag
     2881 gcagagcgcc cgctcatcta cgccggcggc ggcgtggtcc tctcgggagc gtcggccgaa
     2941 ctcctggaat tcgcggaaac cgctgcgatc ccggtgacga cgaccctgat gggtcttggc
     3001 gccgtcccgg gcgaccatcc cctctctctt gggatgctcg gtatgcacgg cacccagtcc
     3061 gcaaactacg cggtgacgga gtgcgacctc ctcctcgccg tcggcgtccg gttcgatgac
     3121 cgggtgacgg gcaggatcgg gacgttcgcg ccgaacgctg cagtcatcca catcgatatc
     3181 gaccctgcgg agatcgggaa gaacaaaccg gtcgacgtcc cgatcgtggg cgacgtgaag
     3241 gcggtgcttc aggcgctcct ccggcggatg cagaagcgcg gcgacacaac gaactgggtt
     3301 gcccggacga acgcctggaa ggcacagtac ccgctcacct accgcgaaga cgaccacctc
     3361 cgcccgcagt acgtcatccg cgagctctcc gatatactga agggcgaggc tatcatcacg
     3421 agcgaggtcg gccagaacca gatgtggacc gccctctact actgcttcaa gaaaccccgg
     3481 acctggatca cctccggcgg ccttggcacg atgggctacg ggttcccggc ggccatcggc
     3541 gcccacttcg cccggccgga cgtgccggtc gtcgatgtcg ccggcgacgg gagtttccag
     3601 atgaacatcc aggagctcgg aacggtggcg cactaccgga tccccgtcaa ggtcatgatc
     3661 ctgaacaaca tgtacctcgg catggtccgc cagtggcagg aactcttcta cgaccgccgc
     3721 tactcctaca cggagctccc gccggtggac ttcgtgaaga tcgcgaacgc ctacggggtc
     3781 gacgggatcc gggtcgacgc gaaggacggt gtcagggagg cgatcgagac cgctcttgcg
     3841 accgacgggc cgttcgtcct cgacttccgg gtcgagcggg aggagaacgt cttccccatg
     3901 gtccccgcgg gtgctgcgat caacgagatg atcggggtgc gccaatgaag ctgcacaccc
     3961 tgagcgttct cgtggagaac cgggcgggag tcttaagccg ggttgccggc atgttctcgc
     4021 gccgggggtt caacatcgag agcctcgccg tcggaacctg cgaagaaccg aacatgagcc
     4081 ggatcacgat cgtcgtgaac ggcgatgacg gcgtcgtcga gcaggtgatg aaacagacga
     4141 acaagctcat cgacgtcatc aaggtctcgg acctgacgga gcgcgagagc gtggagcggg
     4201 agctcgccct catcaaggtg gcgacggaga ccggcccggc ccgcgccgag gtcatgcaga
     4261 tcgccgatat cttccgtgcc caggtcgtcg acgtcggtgc aaagacgctg gtgctccagg
     4321 tgaccgggga tacgggaaag atcgacgcgc tcgagagcct cctgcgccag tacggcatca
     4381 aggaactcgt ccgcacaggt aagattgcca tcctccgcgg cgtaaagacc gtgaagagct
     4441 ctaaataatc ctctttttag tgagatatac tctctgcgcc atcggggcga ttcctccgcc
     4501 ccattgacga tcgcgttatc acatacaacg tcatacctta acgcagcatg atctttggac
     4561 tacctctcca cacgggagtc atcgtgggcg gcgctgccgt tctcgtcatc gtggtgctca
     4621 ccctctgggg gctccggttc agggaggccc cctgaatggc ggacctcacc gtgctcgccg
     4681 tgatcggact ttacttcatc gtgctcatcg gtatcgggag ctgggcctca aaaaagatcc
     4741 acaccaccga ggactacatc ctcgccggaa ggtcgcttgg gttctgggtc ttcaccatcc
     4801 tgatcgtctg ctcgatctgc agcgggatga ccctgctcgg cgtgagcggc tttggataca
     4861 cgtcgggctg gccgggcata tgggagcaga tcttcgttcc gctcgcggcc tcgttctgca
     4921 tcatcgtctt tggggtgaaa ctgaacgccg tcgggaagga acggggctac ctgaccatcc
     4981 aggactacct tgcggaacgg ttcgagagcc cgcgggcgct ccgtggcctc tcggccgttt
     5041 cgggcatcgt cgtctcgctg atctacctcg tcgggcagta tgccgcgatc accatcgtgc
     5101 ttgtctggct cttcggcatc ccccactggc aggcgctcct catatccggt gtcatcatca
     5161 ccgcctacac ggtcgtcggg gggctctacg ccgtatcctg gacgaccctc tttcagggcg
     5221 gcatcctgat cctcggcgtc ctcctgatgg cgccgccggt gatcatgagc gccggcgggc
     5281 tcacccacat caacacggtc cttgccggcg tcgacccgaa cttcgtcggc ccctggttcc
     5341 cggaggcgta tgccccgtac gcctacgcga cgccggagtt cctcttctcg tttgcgatcc
     5401 tcctgatggt cgggctcgcc tgcgcgccgc acgtcgtcaa caacgtcctt gccgcaagag
     5461 aggcacgtta tttcaagtgg gcgccgctcg tcgccttcct gatctacgcg gtcgtgatgc
     5521 tcctcgtcaa gcttgtcggg ttcgccgtcc ggtcgctcgt cgaggagggg aggctcgtcc
     5581 tcccggacgc cgtgaacgcg caggactact ccttcatcgt cggcgtcgag catgcgatgc
     5641 cgaacgtggc gttctgggcg ttctttgcgg tgatcgtcct cgcggcggtg atgtccacga
     5701 ccgaccggct gatgctcacc gtcggcacct cgtttgcgtg ggatatctac aagaacatcc
     5761 tccggccctc ggcatccgac agagaggtgc ttctcgtctc aaagatcgcc ctcgtcctcg
     5821 gggccggcgg cacgctcctg cttgccatca acccgcccga gatgctcgcg tggctgatct
     5881 ggatgggtat cggcgtgatg ctcgcgacgt ttgcggtccc gctgctcgcc gggctctact
     5941 ggcgcggcgc gacagcgggg ggtgcgatcg cgagcatggt ggtcggactc gttgcggcag
     6001 cagccttcgg ctactggcac cagttcgtgg cgccgcttcc cgtgcacttc agcctctttg
     6061 cgctcgcgtt ctccgccctc gccatggtcg tcgcgagccg tctttccacc cggaactccg
     6121 aagagacact cgccaatacc cggaccggct ggttcatcca gtcgcccggc catgacgcgc
     6181 cccgccggca ctgacgccgg ggcactcccg gcgccatttt tgcggtcggg acggcaccgg
     6241 aagacccgcc cacaggggca atttgtgggc aaaggctgtg atgcgcatgc gatagttttt
     6301 gcctgatctg gttgcccgga ccttccatcg gtggggtctc ctcatccgga tcgattgtgt
     6361 tatatgatag cgtgtcacac actgacatag tatgttcttc gcactacctc ttcacacggg
     6421 actcttcgtg ggcggcgctg tcgcgttcgt cctcgtagtc ctcgccctct ggggactccg
     6481 gttcagggag gcctcttgaa tggcggatat tgtaacgctc gccctggtcg ggctgtactt
     6541 cgtcgtgctc atcggtatcg ggagctgggc ctcaaaaaag atccacaaca ccgaggacta
     6601 catcgttgcc ggaaggtcgc ttgggttctg ggtcttcacc atcctgatcg tctgctcgat
     6661 ctgcagcggg atgaccctgc tcggcgtgag cggctttgga tacacgtcgg gctggccggg
     6721 catatgggag cagatcttcg ttccgctcgc ggcctcgttc tgcatcatcg tctttggggt
     6781 gaaactgaac gccgtcggga aggaacgggg ctacctgacc atccaggact accttgcgga
     6841 acggttcgag agcccgcggg cgctccgtgg cctctcggcc gtttcgggca tcgtcgtctc
     6901 gctgatctac ctcgtcgggc agtatgccgc gatcaccatc gtgcttgtct ggctcttcgg
     6961 catcccccac tggcaggcgc tcctcatatc cggtgtcatc atcaccgcct acacggtcgt
     7021 cggggggctc tacgccgtat cctggacgac cctctttcag ggcggcatcc tgatcctcgg
     7081 cgtcctcctg atggcgccgc cggtgatcat gagcgccggc gggctcgatc acatcaacac
     7141 ggtccttgcc ggcgtcgacc cgaactttgt cgaaccctgg ttcccggcgg cgtatgcccc
     7201 gtacgcctac gcgacgccgg agttcctggt ctcgtttggg atcctcctga tggtcgggct
     7261 cgcctgcgcg ccgcacgtca tcaacaacgt ccttgccgca aaggaggctc gttacttcaa
     7321 gtgggcgccg ctcgtcgcgt ttgcgatcta cgcggtcgtt atgttcctcg tcaagttcac
     7381 cgggtttgcc gtccggtcgc tcgccacgga gggggcgatc acgctccccg ccgccgtgaa
     7441 cgcgcaggac tactccttca tcgtcggcgt cgagtacgcg atgccgagcg tgctgctctg
     7501 ggcgttcttt gcggtgatcg tcctcgcggc ggtgatgtcc acgaccgatc ggctgatgct
     7561 caccgtcggc acctcgtttg cgtgggatat ctacaagaac atcttccggc cctcggcatc
     7621 cgacgatgag gtcctcctgg tatcaaaggt cgcagtcgtc ctcggggccg gcggcacgct
     7681 cctgcttgcc atcaacccgc ccgagatgct cgcgtggctg atctggatgg gtatcggcgt
     7741 gatgctcgcg acgtttgcgg tcccgctgct cgccgggctc tactggcgcg gcgcgacaaa
     7801 ggagggggcg atcgcgagta tggcagtcgg gctcatcgcg gccggggtct ttggctactg
     7861 gtccaagttc gtaggatcgc tccccgtaca cttcagcttc tacgcgctcc tgctctcggc
     7921 ccttgccatg atcgccgtga gcctcctcac tgcccggatc tctaccgata ccctcgacaa
     7981 cacccggacc ggctggttca tccagtcgcc cggtcagcct tcacgggctg gcagcaaagg
     8041 ggatgcctgg ctggtcgaac aggatacgcc ccgccagcac tgaggccggg gcgctcccgg
     8101 caccttttct ttttcttctg catgagggtg atggtgcgcc gtgccgatcg ccttcgcaaa
     8161 gtccatattc ctccggggaa ccacatagac tgctgacata gccggcggac agcgagatcg
     8221 ggcgtgcagg agccggcatg ccggccgggt cgagccgggt cgtttgaggt tgccatgagg
     8281 atcgtcatct actacttcac cgggaccggc aactcgcttg ccgccgcaaa aaagatcgcc
     8341 gcagtcctcg gggactgcga gatcgtccct atcgcatcgc tcgcgaacac ccccggggcc
     8401 atcgctccgg aggccggccg gatcgggatc gtctgcccgg tctacttctc cgggctcccg
     8461 gcgatggtga cggcgtttgc aaagcgcctc gataccgcac cggcgggcta cctcttcgcg
     8521 gtcgtcatgc acgggggagg cggaggcgcc gccgtctgcc cggcgggcaa catcgatatg
     8581 gtcgatgggc gaccagtctg gaaccaccgc tgcgaactct gctgcgggtg catccacctc
     8641 tgcccggccg gggctatcca ggccgggagc cgaaccgagg ggaggcagcg ctaccggcac
     8701 ccggacgtga cggttgcgga tatggagagg caggcgggac gaaaccgcac gagaagagcc
     8761 gggtgacagg tcccacgcag cgttcgtaag aggcaattat gccgtattac atcgatgccg
     8821 gaagaataag ccttgacgac ctgcaaaaga ggatcacgga gaccgatccg gttccaggcc
     8881 gtggcggcac ggtcctatgc tttccgcgtg atgtcgtttt gcgccgggga ggtgcgtacc
     8941 cgcccttccc gcaaggtata tcagcccgcc ggcttcagat ccgtatataa gacacaagcg
     9001 gctgaaccat gcagatctcc gtcatctccg ggagtcccaa gggcgagaag agcgtcactt
     9061 tccagtatgt ccggttcctc gagcaggcat tcccggacca caccttcact gtcgtgcatg
     9121 cgggaaggga tatcagggcg attgaagagc gggaagaggt atggcgcggg ctgctcgcta
     9181 cggtggaggg gtgcgagggc gtactctggg caacgccggt ctacgtcatg ctggtcccgg
     9241 cccagctcaa gcgtttcatc gaactggtcg gcgagcggaa cgcggcggag gcattctccg
     9301 ggaagtatgc cgcctgcctc accacctcca tccgcttctt cgatcacacc gcccacgcct
     9361 acctgcacgg ggtctgcgac gacctcggga tgctctacac cggtttctgc tccgcccata
     9421 tggaagacct ccgaaatgag gcgttccagg agcagttcat cctcttcttc tccgatttcc
     9481 tggaggcaat catcgagcaa cggccggtcc agagagtatt tccaccattg cccgcagcct
     9541 ccgcttgctg cgctctaaaa acctcgccgg ccgcggtgga tacccgcgga agacgggtgg
     9601 tcatcctcca cgacgccgaa cccggttccg gccaggaggc gatggtccgg caccttgcgg
     9661 cgtgctacgc gggtccggtc caggtcgccc acgtcaggga cgccgggatg aaaggcggtt
     9721 gcctggggtg ctgccggtgc gccttcgaga acacctgcat ctacgacgac ggtttccggg
     9781 cgttctggaa cgagtatgtc gtcccggcgg atatactggt catggccggg accgtccggg
     9841 accgattctt ctccgccgcc tggaagcagt tcttcgaccg gagtttctca aacggccacg
     9901 tgccggcatt cgagggaaag cagatcgcgt acctggtgga ggggccgctc ggccacctcc
     9961 cgaacctccg ggaggtcctc acgggcctcg cgacgatgga gggggccaac cttgcaggga
    10021 tcgtgaccga cgaatcggac aggatcgatg ccgccctgca caccctcgcc gaacgctccg
    10081 tccggctctc ggaacggggg tatatcgcgc cggccacctt cccggccgtc gccggccaca
    10141 agatcttccg tgacgagatc tggggcggga tgcgggcgat cttcaaggcg gacgaccggt
    10201 attaccggca gcacgggctc tatgactttc ccacccggga ttacccgaag cggatcgtca
    10261 cccgggcgac ctctcttgcg atgagcattc ctgccttcag gcgggatgtg gtagagaaga
    10321 tgcccgccta catggtccgg cccctcgaga aagcgatcga aaccagcacg gtcctcgcga
    10381 agagaagggc aaaataggag cattcctgat cgccggaagg acgcccgatc ctctacgcat
    10441 caccggacgc ctccgcggat cgccggaagg gtgccggggt tgcggcccga tttctccccc
    10501 ggagaggtca actcggtgca gcgcggatgg tcaaccatat aaggcttcgg cattaaacaa
    10561 taggaatgtg aaaacggcgg tactgggcgg cggtctcacc ggggtaacgc tggcccgcct
    10621 gctccacgag cggggcgacg atgtgaccgt tctcgaacgc agcgagacga tcggggggct
    10681 ctgccgctcg aagacggagg cggggttcac gttcgatatc ggggggtcgc acatcatctt
    10741 ctcccgcgac gcggaagccc tctccttcat gctctcggcc ctcggcggga acgccgaccg
    10801 gcggaagcgc aacacaaaga tcctctacaa agggcgttac gtcaaatacc ccttcgagaa
    10861 cggccttatg gatctgcccg cggaggaccg tttcttctgc atcaacgagt tcgtgaggaa
    10921 cctgatcgcc gtcgagaagg gcgaggtccc gccgccggca aactttgccg aatggatcgt
    10981 ctccacgttc ggccggggca tcgccgagtg ctacatgctc ccctataacg agaagatctg
    11041 gaactacccg gccgaccgga tgtcgcacca ctgggtggac gggcggatcc cccgcccgcc
    11101 aatcgaggat atcatccggt cggccatcgg tatcgagacc gagggctaca cccaccaggc
    11161 ggtcttctcc tatccggtgg agggcggtat cgaggcgctg gtcagggcga tcgcagaacc
    11221 ggtcctcccc gccgtccgga ccggctttac ggtcagttcc atccgcgaag agggcgatgg
    11281 gtttgccgtc agcgacggcc gcgagaccgt ccatgccggc cgcctgatat cgacgatccc
    11341 cctccagaac ctgctcccct gcctttcggg tgtcccggcc gacgtgcggg cggcctgcga
    11401 cgccctccgc tacaactcgc tctgctcggt cttcatcggc atcgccggcg aagtccccga
    11461 catatcctgg ctgtatgtcc ccgacgaggc ggccggcctc ttcaaccggg tatcgttccc
    11521 ctcaaactac agcacgaggg tcgcacccgc cggccactca tcgatccttg ccgagatcac
    11581 ctacaacgac ggcgatgcgg tctcccgtat ggcggatgcc gacctgatcg agcacaccgt
    11641 cacgtcgctc gtcgccgccg ggctcatccc ctcgcgggat accgtcgtct acaccggcat
    11701 cgcgcggcag gagttcgcct acgtcgtcta cgatatcgac taccagaaga acatcgggat
    11761 cgtcagggag ttctgccggg agaaggggat cgacctcgtc ggccggttct cccggttcga
    11821 gtacctcaac atggacggct gcatccggag cgcgatcgac tttttgagag gagatggatg
    11881 aaggtcaaca tattcgtcga agatttccgt tttctgcgct acatcgggtg cgcaacggcc
    11941 gcacgaaccc tgtataacca ccttgcccgg ctccccgata tggaggtctc ccgcaacctc
    12001 taccacggcg acttcgacct gacccactac cacactttcg ggccgtgggc gatgtaccac
    12061 ctgaagttca ccgagggcgt gaaggtcctg accgcccact cgaccccacg gctgaacgag
    12121 gggaacgtcg ctttctccaa gcggatcaac acgatatacc cgaagatcta ccggcagttc
    12181 gaccacatca taacgatatc ggagccctgc cgccgcgaga ccgaacagct cgtccccgac
    12241 gtaccaaaga ccctcatacc gaacggcgtc gaccgggcgt acttcaagcg ggacaagaag
    12301 aagaggaagt cgtttcgcga tgagttcgag atcgaacccg gccagagggt ggtgctctcc
    12361 gtcggccagc agaccccccg gaaaggcatc tacgacttct tcaggcttgc cgaccagcac
    12421 cccgacatga cctgggtctg ggtgggcggg ttcccctacg ggacgctctc gaaggattac
    12481 gcgaagatcc agatgctcaa gacccatgcc cacgcaaacg tcatattcac cgggatcatc
    12541 gacgacatat cccgggcgta cagcggggcg gacgtcttct tcatgccgtc gcacgccgag
    12601 acgttcggcc tcgtgatcgt cgaagccctc tcggcgggct tgccggtcat cgcccgggat
    12661 atctttgagt ttaaggagat cttcggcgac tcggtcctct tctttggaga tctcgacgat
    12721 gcgcgggttc tcgtgaacaa tgatgcggcc ctgcggcgct gcgcctcccg tgcccgcgcc
    12781 tcgacggaga attacgacat cacgcttata gcacaacggc atcaacaact gtaccgggag
    12841 ttgatcgagg catgatctcg gtcgtcgtgc cgacgtacaa cgaagagcag aacatagagc
    12901 gctgccttgc gtctcttgcc gaccagacgg tcccgcggga gacctacgag atcatcgtcg
    12961 tcgatgggaa ctcaagggac cggacccgcg agctcgcaga gcccctcgcg gataaggtct
    13021 tcatccagac gagcaaacgg gtggggggag cccgaaacga cggtgcgatg gcggccagcg
    13081 gtgacatcat cgcgacgacc gacgccgact gcatcctccc ccgcgactgg gtggagcgga
    13141 tcgggcaaaa ctttgctgag cgggatatcg tgcagctcta cggcacggtc tacccgatcg
    13201 agggggggct taagaaccac ctctcgcttc tgggtgcaaa cacctttgcg cgtcttggct
    13261 gctacacccg gacaatctac ttcacgctcg ggtgcaacac ggcgtttgac cgtgaggcgt
    13321 tcatccgggc cgggatgtac cgctgcatcg atgccgggga cgaccttgag atagcgcagc
    13381 ggatgcgtaa actcggcaga gtccgcctcg acacccgcct gaaagtcgga ttctcgatgc
    13441 ggcgctacca gcagttcggg acgctgaagt cggtttggga atggttatac atcgttttat
    13501 ccggcggcga cgccggcggc gccacctact cgcagcggga gtataagtag ttccatggag
    13561 aggaccccat ccatcccgga ggcctatgcg agggtggtgc agcgctccga tgcctgtgag
    13621 tttgcccgcc tgctcggtat ggcggtcacg gagatcgagg gcggccgtgt ccgggtggcg
    13681 atgccggcgg agggcacgaa gaacgtccac ggcacgacgc acgggggtgc gatattttct
    13741 cttgccgatc acgcgttcgg catcgccgcg aatatggagg gaatcgacca gatagcgata
    13801 tccgcccgta tccgttactt caccgtcccg gcggcggata cgctcgaagc gatcgcccgc
    13861 agggtctctg agaccgagag gacgtcggtg tacgcggtgg atgtcttctc gggcgaccgc
    13921 ctggtcgcct cgtttgaggg agtagggttc aagataggga ccgttccgga ataggaccgg
    13981 caggtcacat gaccctgcag tgcccgccgc cgtgctttgc aaagtactgc tggtggtact
    14041 cctcggcccg ccagaacgtc cccgcgggct cgatcgcggt gacgacgtgg cgccggaacc
    14101 tccccgattg ctctaccgcc gctctcgacg cccgtgcctc tgcctcctgc tccgtcgtgt
    14161 ggtaaaagat aacggacctg tactgcgtcc cgatgtcggg cccctgccgg ttcagcgtcg
    14221 tcgggtcgtg ggcgttccag aagacgttga ggaggtcgcg atacgagacc tccccggggt
    14281 cgtaggtcac ctgcacgacc tcagtatgcc cggtctttcc ggtgcagaca tcggggtagg
    14341 tcgggttctc ggtgctgccg cccatgaacc ccaccgccgt atccacgacg ccgcggacct
    14401 cccggaacgt ctcctcgatg ccccagaaac accccgcacc gaaggtggcg cgttcgatct
    14461 tcttctcctg tgccatgata tcctcgttct ttctgccctc tctccaaaga atgtatcgct
    14521 gccggccggg gcataccctt atccggggtc gggaccacgt aatctcatga tattcgagtc
    14581 agataccgtg gagatggtgg cccggctgat ggcgctttct gcgcgcaccg caccgaaagc
    14641 ccgggggatc gacgtgatcc ggacgctgat cgtcaccggg gatgaacgga gggtgcttgc
    14701 ccgggcgatg cgcgagtatg gcgagcggca caacgccggg ttcttcatcc gggatgcggg
    14761 gaacgtcgag gcaagcgacg cctgcctcat catcgggtcg ctccttgccg acgccgtggg
    14821 cctcgactgc ggcgggtgcg gctacgccac ctgtgcggag atgctcaaag cgcagggtaa
    14881 tctgcggtcg acaccgttct gcggcccgaa ctgtgttgtc aggatggccg acatcggcat
    14941 cgcggtcggt tcggcggtaa agaccgcgag catccacaac gtcgataacc gggtcatgta
    15001 cacggtcggc gtcggcgcgc tctcgctcgg gtggctggaa ggctgcggcg tcgcctacgg
    15061 cattccgctc cgggcatctg gaaaggatat cttctttg
//
LOCUS       DAOSEE010000043        15089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1577_length_15089_cov_190.142743, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000043 DAOSEE010000000
VERSION     DAOSEE010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 15089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15089
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1577_length_15089_cov_190.142743"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4088)
                     /locus_tag="PLI76_04830"
     CDS             complement(<1..4088)
                     /locus_tag="PLI76_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOI61392.1"
                     /translation="MKPQHGIVLVLLIGLIAGAAAAGDLPPALPHEFYGNVAIDGSPA
                     PAGTVVTAMIGGTACGSVEVTSPGRYGDPDWNLGNRLLVTGTEGQNGETITFLVDGGA
                     ADQTAPFTPGRVTRLDLSFEETVTVPTARFTANVTSGPAPLAVRFADASTGDPDSWFW
                     DFGDGENSAERSPTHTYTTPGTYTANLTVANAAGSNSATATIIVQEAGAVEIVRGPYF
                     TGTTTTSTFVNWMTQEPVAGTVEYADDAYYAVNGGYALSVADTAETAFHRVALEDLTP
                     GTLYHYRIVAGNTTLGDRTFRTFPEDGGFTFIVYGDTQLPANIKIVADRIADEDPLFI
                     LHTGDQVNAVESVDEWNNFFSSNRRLLANTTIYTTLGNHEYNDTVYYETFGLPQWYSF
                     VCADARFAVLDDNFNSNPLSETAWLARDLANDTAWKFVANHHPPYSSTTDRAGGWLYP
                     RAWWGQIMQDNGVTAVFNGHVHAYERYIVDGITYLVTGTGAGSLHPLGPANERPAGYQ
                     NSLVHTLGYTKITVHDNGTATGQFVQVARLDPNNSSQVLEVYPPGTAFETYTLTPRAR
                     GADLAAVSLDVPAGIVAGNACTVNGTVENLGGTVAGACPVTLAADGDLIGNLTVDPLA
                     PGETAEVSFTWTPAAAGEAVLTLSVDPASTVPDIDRTNNVRTVTVTVTEPGAPVANFT
                     ANVTAGDAPLAVQFTDASTGGPTSWSWTFGDGGTSDQQNPVHVYTAAGVYTVNLTVEN
                     DYGASSKQAEITVTKPEPIVFNASVVVYSDEPIVVDNVTYGGSTFFNILAAAGYTYTY
                     AESDAGYTDRKPLLTLTIDSVTYPTKDFGPDYAWKFYETLGTGDDYKYKKALNGYIEQ
                     SGTYYIWFGDTSQFGSWDLPTVDNSIYVVELTVDLQPTRPGAPVANFTANVTAGEAPL
                     AVQFTDLSTNATAWAWNFGDGATSAEQNPVHTYTAAGVYTVNLTVTNDFGSDTEVKTG
                     YITVTEPDTQVADFTGTPTTGDAPLEVRFADNSTGKPVQWLWEFGDGRIATTKNPITT
                     YRQPDTYTVNLTATYGDGSSSRSTKADYITVTRPNPVASFTADQASGNADLPVQFTDT
                     STGSPTSWAWNFGDGATSTDQNPAHTYTTAGTYTVTLKVSRDGGPASTKTGQIVVKPV
                     AGFTAGTTRGYAPFAVQFTDASTGNPDTHSWDFGDGATSSDPNPAHTYTTAGTYTVAL
                     TVTGGGQAHTEVKAAYITAVDPGVPPTAEFRADTTGGYAPLVVRFFDQSQGDVDEWLW
                     NFGDGATSAEQNPVHTYTTAGTYTVTLTAKKGSQSSTITKPAYVTVDNPRPIAGFTAT
                     RLRGEAPLTVQFADTSTGAPPLARSWDFGDGTNSTLRNPTHV"
     gene            complement(4093..7602)
                     /locus_tag="PLI76_04835"
     CDS             complement(4093..7602)
                     /locus_tag="PLI76_04835"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOI61393.1"
                     /translation="MKQHRLVTITLMVLLLVAALAAPVLAAGTTEVRIAKFASDGKTV
                     LNETTVDYHWLEANLPIQGDGVTRYYHQGPVFEGIWEDAHPDKPYDGWNPDEDVQMSM
                     LVKADLGAVMGTDLRDICDYIGGAEEGDEIAVRSSDGWQSKRYPYEYIYDPDPRQGPA
                     VLCWFSGNSSGPDAQEAQGQGYPDTGYDVGMRMVFFADTTTNPWGWHIFGNNDMKECW
                     DKDYWNYGAQYPSAAGTSGKLVNEIRIYSQEEPPEPPIADFSVDIASGDAPLVVAFTD
                     CSEGNPTSWSWTFGDGATSAERNPVHTYIIPGVFTASLTVTNAAGSDTAEFSIIVSGE
                     SPGVLHIVKYANDNATVLGERTVDYHWLAANLPVQGDGVTRYYHQGPVTEAAWKEVFP
                     YQAYKPWDPNERIESAFLDLGAVKGTDLAEICELVGGANDGDLITLVADGAALTLPYG
                     TVYEPNPRQGPAVLCWYREGEGYLPDLLLAYFADNSTNLKAWHVFGNTDMQTCWSEAY
                     WTYNGTLPSAASSSRDRVTEVRIESQEPPAPPVPSFSTNVTTGTPPLAVAFNDTSTGA
                     PTAWSWDFGDGKTSTERNPVHVYAAPAVYPGIYTVTLTATNDVGTNITTVENCITVTA
                     GYASFTANTTGGLPPLAVRFTDASTNAPTSWAWDFGDGATSTDQNPVHTYTAVGTHPV
                     TLTITGSFGSDTFVTTEPIDMIRVLVPGDIGTTTLRVAKVAKNGTVLNETTVDYHWLE
                     ENLPVQGDGVTKYYHQWWIFDENPAYPDPDEANRNSVFNGKGRFGAVKGTDIKDICDL
                     VGGASEGDLIRLNSRDGYKTGLQYDWVYNPHPRQGPATLCWYNAGGGNDQGVGYPDTG
                     YFPSMRLIFFADNSTNPWGLHIFGIYDAYLAWGTWEDATTGWSAKWVDAVVIEVDFYT
                     STPVANFTADVTTGDAPLTVQFTDASTGAESWSWDFGDGATSTDRNTTHSYQSAGTYT
                     VTLTVENSAGSDSATATITVIEPGEPGETGTLTLHPGWNFVSTPKRLVNGANTFAIFK
                     DVDTADHAVLLYDGITGWRSANLTTPFRPLDGAWVYANETCSIPLALAADGPELPPEK
                     DLAKGWNAIGFTGTDPRTAKETLLSLGDSWTTLIGFDAQDQEYGVSILRGASGRHGEE
                     RPMYPMRGYWLYMTDAETLGAVG"
     gene            complement(7721..8818)
                     /locus_tag="PLI76_04840"
     CDS             complement(7721..8818)
                     /locus_tag="PLI76_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61394.1"
                     /translation="MKMRQGITLILLLGLVAGAAAAGEGTPTLPHGFSGGVTIGGSPA
                     PVGTVITATIGGTECGSFQTTETGEYGNPGRSVGDRLLVKATADQSGETITFYVDGVA
                     AQETSVFAIGGGTVLDLSIPGSGGSGNTDNTGGSDNTGGSGNTGGTGSTGNTVTARES
                     AAPTAAPEVQTGQASLATSASGEVLETVAVRTAGGTGTVTIPEGTTALDAGGNPLGEV
                     TCREVAPAEVPAAPPGTTVAVALNCGPAGATFDPPVVLTYTLSAEEWAEIGSATPKVI
                     WYNPGTGKWQEIPATVDPAARTITAEVAHFSIYALAWTVPATTAEAQQTGSPGTPTPT
                     GGEPPWPLIGIGAVILIGATIGGFVYLRKKD"
     gene            complement(8832..12974)
                     /locus_tag="PLI76_04845"
     CDS             complement(8832..12974)
                     /locus_tag="PLI76_04845"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOI61395.1"
                     /translation="MPFNLGTYGPASDSIVRNSQIIRTFTIGLQGGSTNLTLENNTFV
                     DVRTGAALASILMVRDVPNSNIVNNTFTNVTATSGIFYFNSPAATNITIAGNTFDATV
                     GGTTFYFRTAGEGNRIYLNNNVAGVGLYAGNTAPDVIWNTTSPVTYTYNGVERTGYLG
                     NHWLDYTGVDADGDGIGDTPYVVPNGLGTDYAPLMGAWEDGVIASPPPEPKTWYVSIG
                     DDADFTTIQAAVDAASSNDTIVVLPGGYSTFIVNKPYLTIRSEEGPDSAIVNCGSGEG
                     ISNSDNAAGLVLDGLTIVNPGGAGIRLGEYGPAPDSVIRNCIIWNISGELVLKETNTT
                     FENNVLYNPGGGTYAINVQFDDIIFQNNRIEGSSAPYGALAVWEVKNGIFRNNTIANC
                     TGPAALIYLAENCTFTQNSFIDNPFGIWLYQPGDDHTFDCNNFVDTSFVKTWGWSDAF
                     TATFNATAPVTYTYNGVEHTGYPGNFWADYAGVDANGDGIIDEPYVLPDDLGTDYAPL
                     VGAWEDGVIASPAPEPIVVNASVTVYADEPIVVNGTTYGGNSFFNILAAAGFTYTYAE
                     SDAGYGDRKPLLTLTIDGVTYPTEDFGPDYKWKFYDGLGSSAYKYKKALNGYIDNSGT
                     YYIWFGDTSQFGSLDDPTVENSIYVLELNVTLLPTRPAEPGAPVADFTANVTSGELPL
                     AVAFTDASTGAPTTWAWDFENDGTADSTEQSPVHVYPAAGTYTVNLTVGNSYGESSKL
                     AEITVTKPDPIVFNTAVTVYADEPIVVDGTTYDGNTFFNILAAAGYTYTYAESDAGYT
                     DRKPLLTLTIDGVTYPTEDFGPDYAWKFYETLGTGNDVKFKKALNGYISKSGTYYIWY
                     GDMSQFDWWELPSVENSTYVVELNVTLLPTRPGEDGTPAGVTIISPLPNSGYAVGASV
                     RLAATATGDNLTYTWDLGNGDTVVGQDTSYAYPAAGNYTITLTVANDAGSAEASTGIT
                     ILDSDLPYIVLYQGTVDIPAGSTIQAAISPRSGGYVPGEDFVEIPAESVGGMLNASGF
                     IFNLWKSTKPIPTYSLTDITTPDGVYYPNVQDATANLAWRSFDGMTSAATKYDPDVPL
                     ANGTTIYTVYGDYDVVNSDPSGARYVIITTLNIVEPGTANPPVAGFDASTTSGDAPLA
                     VAFTDRSTNAPTSWSWTFGDGATSAEQNPVHTYTAPGTYTVSLMVANTAGSDSATKTI
                     VVTEPGEEADTIVLRPGWNFVSTPKRLADGANTFAIFDGVDTAGRSILLYDGLDYDWR
                     QMGLTDTFRPLDAVWIYANGTSEIPLTFAGGAAELPPQKNLGKGWNAIGFSDTVPESA
                     AATLLSLGNSWATLIGYDAVDQRYESAIIRTTAGETREMQPMQGYWIYTTRAETLAAI
                     SG"
     gene            complement(13354..14622)
                     /locus_tag="PLI76_04850"
     CDS             complement(13354..14622)
                     /locus_tag="PLI76_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61396.1"
                     /translation="MGLIWRVGLWGWACEERLVGGIDMRQHSVLKARVIPAACCLVIA
                     LLFVAAPAMAATTEVTVTKYRDNNYSSVENETTLDLAELQALSSVASGGPLYMQGGTF
                     DYNLSVWAYTPGSMSDFGEQNGTYVRDITDEVGGMNAGDEIWVVASDGYKTYFNHTNV
                     YAPHASQGEMILSWENDTSTVPTYENGIRLFFYTPDDLNFTNENMRDSMASWYWRLVA
                     DKYSPFGVFPSAMGLSSRTVSDLKIYPPHRYDFETGGDTTKWAYWGGVAGEPGSATEP
                     GNSINGISWIAADDNDSYPSSSEYVTGDSAAHRFVFAVVENPDNIEKLDITWIGTGTH
                     ENGVDNGATLYIWNGTHYEQLDTTDSGIEDTLTSTIATNVSKYVVGGNVTVLAKQNGE
                     DDGSLTSVLETDYVKLVVTHHHRNSNFVLP"
BASE COUNT         2454 a         4294 c         5258 g         3083 t
ORIGIN      
        1 aatacgtggg tcggattccg gagagtcgag ttcgtaccgt cgccgaagtc ccatgaccgg
       61 gcaagggggg gagcgccggt tgaggtatcg gcgaactgca cggtaagcgg tgcttcgcct
      121 ctcagcctgg ttgccgtgaa gcccgcaatc ggtctggggt tgtcgacggt gacgtacgct
      181 ggctttgtta tcgtgctgct ctggctaccc ttctttgcgg tcagggtgac ggtgtaggtg
      241 ccggcggtgg tgtaggtgtg gaccgggttc tgttcggcag aggtcgcgcc gtcgccgaag
      301 ttccagagcc attcgtccac atcgccctgg gattgatcga agaaccgcac gacgagcggg
      361 gcataacccc ctgtcgtatc cgcccggaac tctgcggtgg gcggaacgcc ggggtcaact
      421 gcagtgatgt aggctgcctt tacttcggtg tgagcctggc ctccgccggt caccgtcagc
      481 gcgacggtgt aggtgccggc ggttgtatag gtgtgcgccg ggttcggatc gctcgaggtc
      541 gctccgtcgc cgaagtccca ggagtgggtg tccgggtttc cggtggatgc gtcggtgaac
      601 tgcacggcaa acggagcata cccgcgtgtc gtgcctgccg tgaagccggc aaccggtttc
      661 acgacgatct gccctgtttt cgtggaggca ggaccgccgt ctctgctgac cttcagcgtg
      721 acggtgtagg tgccggcggt tgtataggtg tgcgccgggt tctggtcggt cgaggtcgcg
      781 ccgtcaccga agttccaggc ccaggaggtc ggcgagccgg tagaggtatc ggtgaactgc
      841 acgggaaggt cggcattacc gctggcctga tccgcagtga acgacgcgac agggtttggc
      901 cgggtgacgg tgatgtaatc ggccttcgtg ctgcgtgaac ttgagccgtc gccgtatgtt
      961 gccgtcaggt tgacggtgta cgtgtccggc tgccggtagg tggtgatcgg gttcttggtt
     1021 gtcgcgattc ttccatcccc gaactcccat aaccattgaa ccggttttcc ggtggagttg
     1081 tcggcgaatc gcacttcgag cggggcatcg cccgtagtgg gggttccggt gaagtcagcg
     1141 acctgtgtgt cgggctcggt gacggtgatg tagcccgtct tgacctcggt atcgctaccg
     1201 aagtcattcg taaccgtgag gttgacggtg tagacaccgg ccgcagtgta ggtgtggacc
     1261 gggttctgct cggcggaggt cgctccgtcg ccgaagttcc aggcccaggc cgtggcgttg
     1321 gtcgagaggt cggtgaactg cacggcaagt ggcgcctcgc cggcggtcac gtttgcggtg
     1381 aagttcgcaa ccggggcgcc cggtcgggtc ggctggaggt cgacggtaag ttcaacgacg
     1441 tagatcgagt tgtcgaccgt cgggagatcc caggagccga actggctcgt atcgccgaac
     1501 cagatgtagt aggttcccga ttgttcgatg tagccgttta aggctttctt gtacttgtag
     1561 tcatcgccgg tcccgagggt ctcgtagaac ttccacgcgt agtcggggcc gaagtccttc
     1621 gtcggatagg tgacgctgtc gatggtgagg gtcaggagcg gctttctgtc ggtgtagccc
     1681 gcgtcgctct cggcgtaggt gtaggtgtag ccggcggcgg caaggatgtt gaagaacgtg
     1741 ctgccgccgt aggtgacgtt gtcgacgacg atcggctcgt ctgagtagac gacgacggag
     1801 gcgttgaaga cgatcggctc cggtttggtg acggtgatct cggcctgttt ggaggatgcg
     1861 ccgtagtcgt tctccaccgt gaggttgacc gtgtagacac cggcggcggt gtagacgtgg
     1921 accgggttct gctggtcgct ggtaccgccg tccccgaagg tccaggacca ggaggtcgga
     1981 cctccggtcg aggcgtcggt gaactgcacg gcgagcggcg catcgccggc ggtcacgttc
     2041 gcggtgaagt tcgcgaccgg agcaccgggc tcggtgacgg tgacggtgac ggttcgaacg
     2101 ttgttcgtgc ggtcaatgtc cgggaccgtc gatgccgggt cgaccgaaag ggtgaggacc
     2161 gcttcgccgg cggctgccgg ggtccaggtg aaagagactt ctgcggtctc tccgggagca
     2221 agcgggtcga cggtgaggtt gccgatgagg tcgccgtcgg cggcgagggt gaccgggcat
     2281 gcgccggcca cggtgccgcc gaggttttca acggtcccgt tgacggtgca ggcgttcccg
     2341 gcgacgatgc cggccgggac gtccagggag acggccgcga ggtcggcgcc ccgggcccgc
     2401 ggggtcaggg tgtaggtctc aaaggcggtg cccggcgggt agacctcgag cacctgactg
     2461 ctgttgttgg ggtcgagcct ggcgacctgg acgaactggc ccgtggcggt cccgttgtcg
     2521 tggacggtga tcttcgtgta gcccagggta tggacgaggc tgttctggta gccggccggc
     2581 ctctcgttgg ccggcccgag cgggtggagc gagccggcgc cggtgccggt gacgaggtaa
     2641 gtgatgccgt cgacgatgta gcgctcgtag gcgtggacgt ggccgttgaa gacagcggtg
     2701 acgccgttgt cctgcatgat ctggccccac cacgcgcgag gatagagcca tcccccggcg
     2761 cggtcggtcg tggacgagta ggggggatgg tggttggcga cgaacttcca ggccgtgtcg
     2821 ttggcgaggt cccgggcgag ccaggcggtc tctgagaggg ggttgctgtt gaagttgtcg
     2881 tcgaggacgg cgaagcgggc gtccgcgcag acgaaggagt accactgggg cagcccgaag
     2941 gtttcgtagt agacggtgtc gttgtactcg tggttgccga gggtggtgta gatggtggtg
     3001 tttgcaagca gccgccggtt gctcgaaaag aagttgttcc actcgtccac gctctcgacg
     3061 gcgttgacct ggtcgccggt gtggaggatg aagagcgggt cttcgtcggc gatccggtcg
     3121 gcgacgatct tgatgttcgc cggcagctgc gtgtcgccgt agacgatgaa ggtgaagccg
     3181 ccgtcctcgg ggaacgtccg gaaggtgcgg tcgcccaggg tcgtgttgcc ggcaacgata
     3241 cggtagtggt aaagggtgcc cggggtgagg tcctcaagcg cgacgcggtg gaacgcggtc
     3301 tctgcggtgt cggcgacgga tagcgcgtat ccgccgttca cagcgtaata ggcgtcgtct
     3361 gcgtactcga cggtgcccgc gacgggttcc tgcgtcatcc agttgacgaa cgtcgacgtc
     3421 gtcgtggtgc cggtgaagta cgggccgcgg acgatctcta ccgcgcctgc ctcctgtacg
     3481 atgatggttg ccgtcgcgct gttgctcccg gcggcgttcg cgaccgtgag gttcgcggtg
     3541 taggtgccgg gtgtcgtgta ggtgtgggtc gggctccgtt cggcggagtt ttcaccgtcg
     3601 ccgaagtccc agaaccagga gtccgggtcg ccggtcgagg cgtcggcgaa ccggacggcg
     3661 agcggcgccg gcccggaggt tacgttcgcc gtgaaccgcg cggtcggcac cgtcacggtc
     3721 tcctcgaacg aaaggtcgag gcgggtcacc ctgccggggg tgaacggagc ggtctggtcc
     3781 gccgcccctc cgtcgacgag gaatgtaatc gtctcaccgt tctggccctc ggtcccggtc
     3841 acgagcaggc ggttccccag gttccagtcg ggatcgccgt atcttccggg gctcgtcacc
     3901 tcgacggagc cgcaggcggt acctccgatc atcgcggtga cgacggtccc ggcaggtgcg
     3961 ggagagccgt cgatcgccac gttcccgtag aactcatggg ggagggcggg gggtagatcc
     4021 ccggccgccg ccgccccggc gatgaggccg atgaggagga ccagtacaat gccgtgttgt
     4081 ggtttcacct tctcacccga cggcaccgag cgtctccgcg tcggtcatgt agagccagta
     4141 accccgcatg ggatacatcg ggcgttcttc accgtgacgg ccgcttgcgc cgcggaggat
     4201 cgaaacgccg tattcctgat cctgtgcgtc gaaaccgatg agcgtggtcc agctatcgcc
     4261 gagcgagagc agggtctcct ttgcggttct ggggtcggtt ccggtgaagc cgatcgcgtt
     4321 ccagcctttt gcaaggtctt tttcaggcgg gagttcggga ccgtcggcgg cgagggcgag
     4381 cgggatcgag caggtctcgt ttgcgtagac ccaggccccg tcgagcggcc ggaagggtgt
     4441 cgtcaaattg gccgatctcc agccggttat gccgtcgtag agcaggaccg cgtgatcggc
     4501 ggtatcgacg tccttgaaga tcgcaaaggt gtttgcaccg ttaacgaggc gcttcggggt
     4561 cgagacgaag ttccagccgg ggtggagggt aagggtgccc gtctccccgg gctcgccggg
     4621 ttcgatcacg gtgatggtcg cggtcgcgct gtcgctcccg gcagagttct cgaccgtcag
     4681 ggtgacggtg taggtgcccg cgctctgata actgtgggtc gtgttccggt cggtcgaggt
     4741 cgcaccgtca ccgaagtccc aggaccagga ttcagcgcct gtcgaggcgt cggtgaactg
     4801 gacggtgagc ggggcgtcgc ccgtggtcac atcggcggtg aagttcgcga ccggggtgga
     4861 ggtgtaaaaa tctacctcga tgacgaccgc atcgacccac tttgccgacc agcccgtggt
     4921 agcgtcttcc cacgtgcccc aggccaggta ggcgtcgtag atgccgaaga tgtgcagccc
     4981 ccaggggttc gtggagttgt cggcgaagaa gatcaggcgc atgctcggga agtagccggt
     5041 atcggggtag ccgactccct ggtcgttgcc tccgcctgca ttataccagc agagggtggc
     5101 cgggccctga cgcgggtggg ggttatagac ccagtcgtat tgaagacctg ttttatagcc
     5161 gtccctgctg ttcaggcgga taagatcccc ttcggaagct cccccgacca ggtcgcagat
     5221 gtccttgatg tcggtgccct tcacggcgcc gaaccgtcct ttgccgttga agacgctgtt
     5281 ccggttcgcc tcgtccgggt ccgggtaagc cggattctca tcgaagatcc accactggtg
     5341 gtagtacttg gttacgccgt cgccctggac gggaaggttc tcctcgagcc agtggtagtc
     5401 gacggtcgtc tcgttcaaga cggtgccgtt tttagccacc tttgcaacgc gcagggtggt
     5461 ggtcccgatg tcgcccggga caagcacccg gatcatatcg atgggttcgg tcgtcacgaa
     5521 cgtgtcgctc ccgaaggagc cggtgatggt gagggtgacg gggtgggtgc cgacggcggt
     5581 gtaggtgtgg accgggttct gatcggtcga agtcgcgccg tcgccgaagt cccaggccca
     5641 ggaggtcggg gcgtttgttg aggcgtcggt gaaccggacg gcaagcggcg gcaacccgcc
     5701 ggtcgtgttc gcggtgaagc tcgcgtatcc tgcggtgacg gtgatgcagt tctccacggt
     5761 ggtgatgttg gtgccgacgt cgttcgtcgc ggtgagggtg acggtgtaga tgcccgggta
     5821 gacggcgggg gcggcgtaga cgtggaccgg gtttcgttcg gtcgaggtct taccgtcgcc
     5881 gaagtcccag gaccaggccg tcggcgctcc cgtggaggtg tcgttgaagg cgaccgcgag
     5941 aggcggcgtt ccggtggtca cgttggtgct gaaggagggg accgggggcg cggggggctc
     6001 ctggctctcg atccgcacct cggtgacccg gtcccgggag gaggaggcag cggacggcag
     6061 ggtgccgttg taggtccagt aggcctcgct ccagcaggtc tgcatgtcgg tgttgccgaa
     6121 gacgtgccag gccttgaggt tcgtggagtt gtcggcgaag taggcgagga ggaggtcggg
     6181 caggtatcct tcgccctcac ggtaccagca gaggaccgcc gggccctggc gcgggttggg
     6241 ctcgtagacc gtcccatacg ggagggtgag cgcggcgccg tccgcaacga gggtgatgag
     6301 gtcgccgtcg ttcgccccgc cgacgagttc gcagatctcg gcgagatcgg tgcccttcac
     6361 ggcgccgagg tcgaggaagg cgctctcgat ccgttcattg gggtcccagg gcttgtaggc
     6421 ctggtagggg aagacctcct tccaggccgc ctcggtgacc gggccctggt gataatagcg
     6481 ggtgacgccg tcgccctgga ccgggaggtt tgccgccagc cagtggtagt cgaccgtcct
     6541 ctcgcccagg acggtggcgt tgtcgtttgc gtacttcacg atgtggagca cgccggggct
     6601 ctctccggag acgatgatcg agaattccgc cgtgtcgctg cctgcggcgt tcgtgaccgt
     6661 caggctcgcg gtgaagacgc cggggattat gtaggtgtgg acggggttcc gctcggcgga
     6721 ggtcgcgccg tcgccgaacg tccaggacca ggaggtcggg ttgccttcgg agcagtcggt
     6781 gaacgcgacc acaagcgggg cgtcacccga ggcgatgtcg acggagaagt cggcgatcgg
     6841 cggttccggg ggctcttcct ggctgtagat tcggatctcg ttgaccaact tgccggaggt
     6901 gccggcggcc gaggggtact gcgccccgta gttccagtag tccttgtccc agcactcctt
     6961 catgtcgttg ttcccgaaga tgtgccagcc ccaggggttc gtggtggtgt cggcaaagaa
     7021 gaccatgcgc atgccgacgt catacccggt gtccgggtag ccctggccct gcgcctcctg
     7081 ggcgtcgggg cctgatgagt tgccgctgaa ccagcagagg accgccgggc cctggcgcgg
     7141 gtcgggatcg tagatgtact cgtaggggta gcgcttgctc tgccacccgt cgctgctgcg
     7201 gacggcgatc tcgtcgccct cttcggcgcc gccgatgtag tcgcagatgt cccggaggtc
     7261 ggtccccatc accgccccga ggtcggcctt gaccagcatg ctcatctgga catcttcgtc
     7321 ggggttccag ccgtcatagg gtttgtcggg gtgggcatcc tcccagatgc cctcgaagac
     7381 cgggccctgg tggtagtagc gggtgacgcc gtccccctgg atggggagat tcgcttccag
     7441 ccagtggtag tcgacggtcg tctcgtttaa gacggttttc ccgtcgcttg cgaactttgc
     7501 gatgcgcacc tcggtggtgc ccgccgccag caccggcgcc gccagagcgg cgaccagcag
     7561 cagcaccatg agcgtgatgg ttaccagtcg gtgttgtttc atgtggttca actcgcgttg
     7621 ttggattgtc tgtgaggttt cggtcctacg ttcgtgcggg gatcccgtcc gggtgcggcg
     7681 ccccggggcc gcacaacctg gagatctaaa aaaagaggca tcagtccttc tttctgagat
     7741 acacaaagcc tccgattgtg gcgccgatca ggatgaccgc gccgataccg atgagcggcc
     7801 agggcggttc tccgccggtc ggcgtcgggg tgccgggcga accggtctgt tgtgcctccg
     7861 ccgtcgtcgc cgggacggtc catgccaggg catagatgct gaagtgggcg acctcagcgg
     7921 tgatcgtccg cgcggcaggg tccacggtgg cggggatctc ctgccacttc ccggttccgg
     7981 ggttatacca gatcaccttc ggtgtggcgc tgccgatctc tgcccactcc tcagcggaga
     8041 gggtgtaggt gaggacgacc ggcgggtcga acgtggcgcc cgcagggccg cagttgagcg
     8101 cgacggcaac cgttgttccc ggcggggccg ccggaacttc ggccggagcg acctctctgc
     8161 aggtcacctc gccgagcggg ttgccgccgg cgtcaagggc ggtggtgccc tccggtatcg
     8221 tcacggtgcc ggtcccgccg gcggttctga ccgccacggt ctcaagcacc tccccggacg
     8281 cagatgtggc aagggaggcc tgcccggtct gcacctcggg agcggcggtg ggggcggccg
     8341 attcgcgggc ggtcacggta ttgccggtgc tcccggtacc cccggtgttt ccggaaccac
     8401 ctgtgttgtc ggaaccgcct gtgttgtcgg tgttgccgct gccgccgctc cccgggatgc
     8461 tcaggtcaag gaccgtccct ccgccgattg cgaagaccga cgtctcctgt gcggcgacac
     8521 cgtcgacgta gaacgtgatc gtctcaccgc tctggtccgc ggttgccttc acgagcaggc
     8581 ggtcccccac agaccggcca ggattaccgt attctccggt ttccgtcgtc tggaacgagc
     8641 cgcattccgt gcccccgatc gtcgccgtga tcacggttcc gacgggtgcg ggggagcccc
     8701 cgatcgtcac gccaccggag aaaccgtggg gtaacgtggg ggtgccctcc ccggccgccg
     8761 ccgcaccggc gacgaggccg aggaggagga ttagtgtgat gccctgtcgc atcttcaccc
     8821 tgcaccaccc tctagccgct gatggcggca agcgtctccg cacgggtcgt gtagatccag
     8881 tagccctgca tgggctgcat ctcccgcgtc tcgccggccg tggtgcgtat gatcgcgctc
     8941 tcgtaccgct ggtcgaccgc gtcgtaaccg atcagggtgg cccagctgtt cccgagcgac
     9001 agcagggtgg ccgccgcgga ttcggggacg gtgtcggaga acccgatcgc gttccagcct
     9061 ttaccgaggt ttttttgggg cggaagttcg gcggcgccgc cggcaaaggt gagcgggatc
     9121 tcggaggtgc cgttcgcgta gatccagacc gcgtcgagcg gccggaaggt gtcggtcagg
     9181 cccatctgcc tccagtcgta gtccagcccg tcatagagca ggatcgagcg gccggcggtg
     9241 tcgacgccgt cgaagatcgc gaaggtgttc gcaccgtcgg cgaggcgctt cggtgtcgag
     9301 acgaagttcc agccggggcg gaggacgatg gtatccgcct cctccccggg ttcggtcacg
     9361 acgatggtct tcgtcgcgct gtcgcttccg gcggtgttcg cgaccatgag gctcacggtg
     9421 taggtgccgg gggcggtgta ggtatggacc gggttctgtt cggcggaggt cgcaccgtcg
     9481 ccgaaggtcc aggaccagga ggtcggcgcg ttggtcgagc gatcggtgaa ggcgaccgca
     9541 agcggcgcgt cgccggacgt ggtgctcgca tcaaagccgg cgaccggcgg gtttgccgtg
     9601 ccgggctcga cgatgttgag cgtggtgatg atgacgtacc gggcgccgct cgggtcggag
     9661 ttgacgacat cgtagtcgcc gtagacggtg tagatggtgg tgccgtttgc aagcgggacg
     9721 tccgggtcgt acttcgttgc ggcagaggtc atgccgtcga aggatcgcca ggcaaggttc
     9781 gccgtcgcgt cctggacgtt cgggtagtag accccgtcgg gggtggtgat gtccgtcagc
     9841 gagtaggtcg ggatcggttt cgtactcttc cagaggttga agatgaagcc gcttgcgttc
     9901 agcatgccgc cgacgctctc cgccgggatc tcgacgaagt cctcgccggg gacgtagccc
     9961 ccggacctcg gcgagatggc ggcctggatc gtgctgccgg ccgggatatc gaccgtgccc
    10021 tggtacagga cgatgtaggg aaggtccgag tcaaggatgg tgatcccggt cgatgcctcg
    10081 gccgagccgg catcgtttgc cacggtcagg gtgatggtgt agttgcccgc agcagggtac
    10141 gcgtatgaag tgtcctgccc gacgacggta tcgccgttgc cgagatccca ggtgtaggtg
    10201 aggttgtcgc cggtcgcggt cgccgcaagg cgaaccgagg cgccgacggc ataaccgctg
    10261 ttcggcaacg gggatatgat cgtcacgccg gccggggtgc catcctcacc gggccgggtc
    10321 ggcaggaggg tgacgttgag ttcgacgacg taggtcgagt tctcgaccga cgggagttcc
    10381 caccagtcga actggctcat gtcgccgtac cagatgtagt aggtgccgga tttgctgatg
    10441 tagccgttta aggctttctt gaatttgacg tcgttaccgg tcccgagggt ctcgtagaac
    10501 ttccacgcgt agtcggggcc gaagtcttcc gtcggatagg tgacgccgtc gatggtgagg
    10561 gtcaggaggg gctttctgtc ggtgtagccc gcgtcgctct cggcgtaggt gtaggtgtag
    10621 ccggcggcgg caaggatgtt gaagaacgtg ttgccgtcgt aggtggtgcc gtcgacgacg
    10681 atcggctcgt ccgcgtagac cgtgacagcg gtgttgaaga cgatcgggtc aggtttggtg
    10741 acggtgatct cggccagttt ggaggactcg ccgtagctgt tgccgaccgt gaggttgacg
    10801 gtgtaggtac cggccgcagg gtagacgtgg accgggctct gctcggtgct gtcggcagtg
    10861 ccgtcgttct cgaagtccca ggcccaggtg gtcggggctc cggtcgaagc gtcggtgaac
    10921 gccaccgcaa gcggcagctc gccgctcgtt acgttcgccg tgaagtcagc caccggggcg
    10981 cccggttcag ccggccgggt cggcaggagg gtgacgttga gttcgaggac gtagatcgag
    11041 ttctcgacgg tcgggtcgtc cagggagccg aactggctcg tatcgccgaa ccagatgtag
    11101 taggtccccg agttgtcgat gtagccgttc agggctttct tgtacttgta ggcgctcgaa
    11161 ccgaggccgt cgtagaactt ccacttgtag tcggggccga agtcctccgt cgggtaggtg
    11221 acgccgtcga tggtgagggt caggagcggc tttctgtcgc cgtaacccgc gtcgctctcg
    11281 gcgtaggtgt aggtgaaccc cgccgcggcg aggatgttga agaacgagtt gccgccgtag
    11341 gtggtgccgt tgacgacgat cggctcgtcc gcgtagaccg tgacggaggc gttgacgacg
    11401 atcggctcgg gggccggcga ggcgatgacc ccgtcctccc acgcgcccac caggggtgcg
    11461 tagtcggtcc caagatcgtc ggggaggacg tagggttcat cgatgatgcc gtcgccgttc
    11521 gcgtcgacac ccgcgtagtc cgcccagaag ttgcccgggt acccggtgtg ctccacgccg
    11581 ttgtaggtat aggtcaccgg ggcggtggcg ttgaaagttg cggtgaacgc atcgctccaa
    11641 ccccatgttt tgacgaatga agtatcgacg aaattattac agtcgaaggt gtgatcgtca
    11701 cctggctgat acagccaaat cccgaaggga ttgtcaatga aactgttttg cgtgaaggta
    11761 cagttttcgg ccagatagat cagagcagct ggcccggtac aattcgcgat tgtgttattc
    11821 ctgaagatcc cattcttaac ttcccagaca gcaagagcac cgtatggggc gcttgaacct
    11881 tctatcctgt tattctggaa tataatatcg tcaaactgta cattgatagc atacgtaccg
    11941 ccaccagggt tgtagagaac attattctca aaagtggtat tagtctcttt aagaacaagt
    12001 tcaccgctta tattccagat gatgcaattc cggataaccg aatccggtgc aggaccatat
    12061 tctccaagtc ggataccagc tccgccagga ttcacaattg tcaacccgtc aagaaccaat
    12121 cctgcagcgt tatcactgtt ggagattcct tctccactgc cgcaatttac aatggcagaa
    12181 tcgggcccct cctcacttcg gatagtaagg taaggtttgt ttacaatgaa tgttgaatag
    12241 cccccaggca gaacaacgat cgtatcattg gacgaggcgg cgtcgaccgc cgcctggatg
    12301 gtggtaaagt cggcatcgtc gccgatggag acataccagg ttttcggctc cgggggcggc
    12361 gaggcgatga ccccgtcctc ccacgcgccc atcaggggtg cgtagtcggt gccgaggccg
    12421 ttgggaacga cgtagggcgt atcgccgatg ccgtcgccgt cggcgtcgac acccgtgtag
    12481 tccagccagt ggttgccgag gtaccccgtc cgctctacgc cgttgtaggt gtaggtcacc
    12541 ggggaggtgg tgttccagat gacgtccgga gcggtgttgc ccgcgtaaag accgacgccc
    12601 gccacgttgt tattcaggta gatccggttc ccttcaccgg cagtacggaa gtaaaaggta
    12661 gttcccccaa cagtagcgtc gaaggtgttt ccggcgatgg tgatgttggt tgccgctggt
    12721 gaattgaaat agaagattcc tgacgtggct gtgacgttgg tgaacgtgtt attgacgatg
    12781 ttgctgtttg gcacatctct gaccataagg atactggcaa gggctgctcc cgtacgaaca
    12841 tccacgaagg tgttgttctc aagcgtgaga ttggtggacc ctccttgaag ccctattgtg
    12901 aaagttctta tgatctggct gttccggacg atcgaatccg atgcaggacc ataagttcct
    12961 agattgaacg gcatcccccc cgcattgatg aatgtaaacc catcaaggat cgtccctgtc
    13021 gcattacaat tacttgctgt cggtattctg gatgctccat ttagaacgac aggcccgtca
    13081 ttttcaagag cctgaaacgt caggtagggc ttttcaccaa ttagctcaaa tcctgaatat
    13141 gtcccggaga agacaacgat cgtatcatta gacgaagcgg cgttggccgc tgcttggagg
    13201 ttggtaaaat cagtccccgc gtcgacatcg ccgtcgacgt accaagtcgc cgccgatgca
    13261 ggaaatgcga tacaaagcag gccaagtaat atgactaaag caaggtttct tttcatcgta
    13321 atcaccgtaa atgggaaaaa gggttagggt tggttagggt agtacaaagt tggaattcct
    13381 gtggtggtga gtcaccacga gtttcacgta gtcggtttcg agcactgatg ttaaagatcc
    13441 atcatcttca ccattctgct tcgcgagcac cgtgacgttg ccgccgacga cgtatttgct
    13501 gacattcgtg gcgatggtac tggtgagcgt gtcttcgatg ccgctgtccg tggtgtcgag
    13561 ctgctcgtaa tgggtaccgt tccagatgta aagcgtcgca ccgttgtcga cgccattctc
    13621 atgtgtgccc gtgccgatcc aggtgatgtc gagtttctcg atgttgtccg gattctcgac
    13681 gacggcaaag acgaagcggt gagcggcgga gtcacctgtc acgtattcac tagaagacgg
    13741 gtacgaatcg ttatcatcag cagcgatcca cgagatgccg ttgatggagt tgcccggctc
    13801 ggtcgcgctg cccggttcgc cggccacacc accccagtac gcccacttgg tcgtgtcgcc
    13861 gccggtttcg aagtcgtagc ggtgcggcgg gtagatcttc aggtcgctga cggtcctcga
    13921 cgagaggccc atcgccgacg ggaatacacc gaacggcgag tacttgtccg caaccagcct
    13981 ccagtaccac gaggccatcg agtcgcgcat gttctcattg gtaaagttca ggtcgtccgg
    14041 cgtgtagaag aagagcctta ttccgttctc gtaggtggga acggtgctcg tgtcgttctc
    14101 ccaggagagg atcatctcgc cctggctggc gtgcggcgcg tagacattgg tgtggttaaa
    14161 gtacgtcttg tagccgtcgg atgccacaac ccagatctcg tcgcccgcgt tcatcccacc
    14221 gacctcgtcg gtgatgtcgc ggacgtaggt gccgttctgc tccccgaagt cggacatgct
    14281 gcccggggtg tacgcccaca cactcaggtt gtagtcaaag gtcccgccct gcatatacag
    14341 cgggccgccg ctcgcgaccg acgacaatgc ctggagctcc gcaaggtcca gggtcgtctc
    14401 gttctccaca gacgagtagt tgttgtcgcg gtacttcgtt accgttacct ccgtcgtcgc
    14461 cgccattgcc ggcgccgcga cgaacagcag ggcgatgacc aggcagcagg ctgccggaat
    14521 caccctcgct tttaggactg aatgttgtct catgtcaatg cccccaacaa gccgctcctc
    14581 acaggcccaa ccccacaggc cgaccctcca gatcaacccc acaggccgat cctcgcaggc
    14641 cggcttgata tgagatcaat taaactccat aaacttaagc cttttgttaa ataatattca
    14701 tttacacagc acgtcagacg ataagggttg tgcgcaacgc ccggaaagag aaaacagagc
    14761 accggatcca aaatggcact cgtgccctcc gatcatcgat ttaacccttg tggttgcaca
    14821 actccccggc ggccccggga ccgtaaccag ccccacccgc cgggcggggc gggggacctt
    14881 gcggggcatc ggcgggcggg cagagccctc gtcgaaaacc gggggaaaat gtcattttcg
    14941 cccgcggcag cagcggaacc gggtgccggg gttcccggcg cgcgcgggca ccccgcaagg
    15001 gcggcgttgc cgcgagagcg cccggcgggg tccggaggaa tcaacgagcg ctgatgttcc
    15061 gggccgatgt ttcgcccgcg ggtcgggga
//
LOCUS       DAOSEE010000044        14866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1601_length_14866_cov_236.479441, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000044 DAOSEE010000000
VERSION     DAOSEE010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 14866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14866
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1601_length_14866_cov_236.479441"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(252..1784)
                     /locus_tag="PLI76_04855"
     CDS             complement(252..1784)
                     /locus_tag="PLI76_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOI61397.1"
                     /translation="MDYRTVTALLVIVTVLLLSAAGCTATTEENIPGAASISPADEAY
                     ARGVAAYADASYRTAEEYFAEAYALSTGAGDPDRARIARDAMFRANRTYTEYPFNRSA
                     AEAALAETIPGITDAAIADWLDNRAQAIVSENETLYFDDVAGNYLYAHPDEMQKQNAR
                     DLDFGYIARYAWSEDQPEGTGPYVNPVRYAGVERLELPREALPSSGVLKIWFPLPIET
                     DSQRNVTVTNLSCPDYIVAGPFTTGPIGYVYYEIPAGAVNGDLILSADIDFVSYEQIF
                     GDIDPALVGKYNTSDPDYLLYTRSERNIEITDAIREKAREIVGNETNPHLAAQMIYYY
                     IIETYPYSHVPHYSLDARVPKVAESTYMFETGHGDCGTQSMLFAALCRSLGIPARTPG
                     GYQMLMAEAPGTHFWAEYYLPGYGWVPNDPTVAEAADWTVIPEERQSAFKDYYAANLD
                     PARLVFQKSVDIPMDPALPDDVAVFRVVRQFPAVVSDTADYDLDYFWQGNFTVSLAAV
                     DQ"
     gene            2148..2834
                     /locus_tag="PLI76_04860"
     CDS             2148..2834
                     /locus_tag="PLI76_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61398.1"
                     /translation="MQSILRYLLAVFLVACCFVLPASAQPPIGGDNGWYAVNCNVNGA
                     QVYFGNDYKGTIEAGVLYVPVYTTGTPYQTFRVEKEGYTTHYGDVISVPGKGETFDLY
                     ATLNPIQPTQEPLIGGDIGWYTVHSNVDGATVQFDNDVKGTISKGILTVQVYTTGTPY
                     RTYTLTKDGYTTYTGSIDRYPGKGETVDLYATMNPAPTPAPKSPLSPALAGSALLIAG
                     LLLIAGRKNN"
     gene            complement(2965..4548)
                     /locus_tag="PLI76_04865"
     CDS             complement(2965..4548)
                     /locus_tag="PLI76_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOI61399.1"
                     /translation="MAVDTGLFIAITLIYLVAVIGLGYLGYRRTKGDDDYMVAGRSVH
                     PVVLALSYGATFISTSAIIGFGGVAAQLGMGMIWLTVLNIGLGIFIAFVIFGKKTRKI
                     GSKLGAVTFPDLMGKCYQSPFMRYAAGLVIVVAMPLYTAAILIGGAQFITSTLQIPYT
                     TALIAFAAIVALYVVLGGLLAVMYTDALQGGIMLIGMTVLLVLTYIQLGGVIEAHTAL
                     AAMSDLVPQGLAAGGMTGWASMPALGSSIWLTMVTTLVLGVGIGVLAQPQLVVRFMTV
                     KDSRALNRAVLVGGPFILMMTGVAFTVGALTNVYFYKTQGVIALAAATEGNVDTIIPN
                     FINASMPDLFVVIFMLALLAAAMSTLSSLFHVMGTSLGFDVMRRTGSKGASMRLIQVA
                     TVVMIVASVVIAFVMPGSIIARATAMFMGLCASAFLPAYAHAMYSSRPSLFAAKVSLI
                     IGAVVWFLWTAFVHIKESATLGLSNLIFGVPAILPAPWQDVDPLVIALPLSLAALMVG
                     LVLDRHRVSVEEEGPTGAA"
     gene            5206..5598
                     /locus_tag="PLI76_04870"
     CDS             5206..5598
                     /locus_tag="PLI76_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61400.1"
                     /translation="MELHLVRDFGAGDIEAIEVSPAVLEIEVEPRMVDEADRILMMHS
                     VPGRFVYAAGRYPGQFRVEIGESSDLDRIGEALLAITELPGSTPPSYAVRDLIADLYR
                     RREDALERKEADTIEDEIALGLYDDEDW"
     gene            complement(5675..5746)
                     /locus_tag="PLI76_04875"
     tRNA            complement(5675..5746)
                     /locus_tag="PLI76_04875"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5711..5713),aa:Arg,seq:ccg)
     gene            complement(5781..6830)
                     /gene="hypD"
                     /locus_tag="PLI76_04880"
     CDS             complement(5781..6830)
                     /gene="hypD"
                     /locus_tag="PLI76_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HOI61401.1"
                     /translation="MAVGKDILTALFDLVDRDITLMHICGTHEAAIARAGLRSILPER
                     LKIVMGPGCPVCITPQGEIDAALDLVERGCTVATYGDLLRVPGSKGSLESSGGDVRVV
                     QGIHRAVEIAEREPDREVVFISVGFETTAPTVAATILSRPPENFSILSCHRLVPPAMA
                     WLLARGEAALEGFLLPGHVCAVMGYKEYEQFPVPQVVAGFEPEDILLGLLMAVRQVRE
                     GVHRVENAYPRAVSREGNVKAQRLMYEAFEPFDVEWRGFPVIPASGLRLKPEFEDYDA
                     QKKFDIEIRHVDKHSACICDKVLRGVARPSDCRLFAKACTPRTPIGPCMVSHEGACKI
                     WHLYESRRASNPPSR"
     gene            complement(6894..7826)
                     /locus_tag="PLI76_04885"
     CDS             complement(6894..7826)
                     /locus_tag="PLI76_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOI61402.1"
                     /translation="MAYRYLFGPVPSRRLGRSLGIDLVPQKTCTFDCAYCECGRTTVL
                     TLERREYVPTDRVIAELDDCLAKAPDIDYVTFAGSGEPTLHSGIGDIISFIKDRYPRY
                     RVAVLTNGALFVDPDVRAALMRADLVVPSLDAVSEDIFRDINRPSPGITAGQVVAGLV
                     AFSREFPGVIWLEVFIVPGKNDTDEELLRLRDAVAVIAPDRVQVNTLDRPGTDIRVRR
                     ASPRTLERARTIIGGNAEVIGAASCEPATAPEMRDVADMLLATLRRRPCTVADLSRIS
                     GLRPAEVTKHLRILEEKGAVEPVREQRGVFYRAA"
     gene            complement(7900..9081)
                     /locus_tag="PLI76_04890"
     CDS             complement(7900..9081)
                     /locus_tag="PLI76_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61403.1"
                     /translation="MADGFIPEIVISNQLDKSKAEIFSFIESNAEAIDENFPSFFGRV
                     VTILDRAGVPIPDAAHDEFIDAIAYRVFDVSKRAGDVKFVNRVCEIACGMKRKKERKA
                     GVHLAAAVKLMKIGMPLTAAAYLQPYRRHDAVVGCWHAYCYYTLYKEGSAEPGYSAAE
                     RWNYLKLARQHLDELGQLRPGLWRLVRGELEQDRWLAEPFWMMIFLATEWMPDNRWFL
                     EIGLSRAKQENNSVALVKLLQIGLVRFPGDMLFYREAYNLKFDQGDLADALGLINDML
                     QRFPDDLEPIYYGLRTTLYLPREGEFNGFRTLAEEKKMPGHVLCLIDYVYAFLRGRKE
                     QANLCLDEFRRKYPSLSYYSDLLRFATTADLPARPEGINQAIFSSVDQFCKRMLKIGD
                     T"
     gene            9291..10022
                     /locus_tag="PLI76_04895"
     CDS             9291..10022
                     /locus_tag="PLI76_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOI61404.1"
                     /translation="MFGISTYCLHHEPLSLALERLAPLTDCVEVMDEGLHFLESAEPL
                     ESYSYLYFIHAPARGINIASILEPIRRASVEVLTQTFAIAAEVGADVVIHPGYYAWSQ
                     ERERAAGRFRQSLDELTAAAADLSVTFFVENMGNWEYFFLRSCEDLPLVDGIGLALDV
                     GHAHLNGCLDCFLTHPAAHFHLHDNDGTADTHSPVGAGTIDFTAVMDAVARSNVIPIV
                     EVETLDGVTASIDALTAIDAAARGG"
     gene            10188..10259
                     /locus_tag="PLI76_04900"
     tRNA            10188..10259
                     /locus_tag="PLI76_04900"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10220..10222,aa:Thr,seq:ggt)
     gene            10422..11600
                     /locus_tag="PLI76_04905"
     CDS             10422..11600
                     /locus_tag="PLI76_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOI61405.1"
                     /translation="MSPTIDRPPLIYLNNAATTWPKPPGVLAAITQSLALPVFGSGRT
                     TGSQGEDYVTLAREAIAGLLAADPPEHVVFTSSATDSLNILIQGFLAGCDAGCHVLTT
                     ELDHNSVLRPLHELARQGRIRLTVLPFEDGAVSPDTVEAAITPDTRLMVTAHGSNVLG
                     SVQEIARIGKILHDHDISFIVDGAQTAGHIPVDLWSLPVDAYVFTGHKGLFGIPGTGG
                     FYVRDPETIAPTRYGGTGTDSFSLFQPRDMPERFESGTHNYPGLAALAAGAEYIAGIG
                     VETIAEKAERQTAFIIGELKEEPNVTVYNESPSLPIVSFNIRGMENDDVGFILARAYG
                     IVARTGLHCAPLAHRAIDGGRGSVRLSLSWFTTDEECRVAARAIREIAQHADPSVGSP
                     "
     gene            11572..11940
                     /locus_tag="PLI76_04910"
     CDS             11572..11940
                     /locus_tag="PLI76_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61406.1"
                     /translation="MRILRSVRHKSCADGSFMKEFLLDAPVTAGFFAYLENFGEVEAL
                     RNVGEGFYKFEKTDWFSIKGMNGDTTVEVRFKKEAMDLTADFVYLLFSSYREGAADLS
                     LLKQREQAIEKRVKERLYGP"
     gene            11946..12518
                     /locus_tag="PLI76_04915"
     CDS             11946..12518
                     /locus_tag="PLI76_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOI61407.1"
                     /translation="MPGDIFEQFAEDYDRWFDEHRAEYRAELARVRRLLPRPDARAVE
                     VGAGSGRFAAPLGIPIGIEPSPALGRMARERGIEVIRGRAESIPLRDGACSSALVVTV
                     ICFLDDPVPAFREIHRILAPQGTLVTGFLEREGPIAQKYLHEEGKHRFLSRARFYSPD
                     EVRELLGRTGFCVAGAESQAGFSVLTARKA"
     gene            complement(12562..13413)
                     /locus_tag="PLI76_04920"
     CDS             complement(12562..13413)
                     /locus_tag="PLI76_04920"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOI61408.1"
                     /translation="MGELTQDLRAQRDAWDEANERHRGNPLRSVVSVDDLNTMYIDGS
                     QKRLVSRAVRLEPHFTVLDYGCGVGRWTLWFAQEVHRVVGVDLSPGMVEAARRMAGNT
                     GIQNAEHHTIEGMPFPFEDDAFDLVNAVWVLRYILDDDELARTVEEICRVVRPGGHVT
                     FIEMIAKKEPEFKEHEGVFTGAAVYRRLEQYRSLFEGCGMMMKMSAVSSASPLYWLYA
                     LIRDTAKRRSLPDPFSPLAPSIVSASLATESLTATPMRLLADRGLIRCRHRFLCLQKP
                     GGDRTRS"
     gene            complement(13515..14348)
                     /locus_tag="PLI76_04925"
     CDS             complement(13515..14348)
                     /locus_tag="PLI76_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61409.1"
                     /translation="MVRKKRLLIIAATALAAFSIVFAALTVGLLYYSGSSRPSYHYSL
                     TLSTSGPIEDAVLLIPLPSRYDPDSGTNVTPVNMSLASFTNFDGENISVKVEYIGGIP
                     VLNISADRIDPVYTNRIVPIGIMPGQNESELPKPTHVYSNRYSEETPVLVDMELHLPG
                     ISPDHDIETRAPIGVEPLFMPCRVIGNLDGSGGFVDKNHYVSPGSTGYIVEVPFTLSF
                     DADDENVLTISSGFLGTNQRWILGWQSNSYSESLRHEFKGPCNGTYPTRGILLTGKGA
                     Y"
     gene            14533..>14866
                     /locus_tag="PLI76_04930"
     CDS             14533..>14866
                     /locus_tag="PLI76_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOI61410.1"
                     /translation="MPYVTVGKENSGNIDLYYEDHGAGRPVVLIHGWPLSSKSWEKQV
                     PALIDAGYRVVAYDRRGFGNSAKPAFGYDYDTLAEDLHKLMTELDLTGATLVGFSMGG
                     GEVARYLGT"
BASE COUNT         2825 a         4512 c         4707 g         2822 t
ORIGIN      
        1 gggctcaggg caacggtcgt caggtgccgg ccgttggcga agatcgccac cggggggtga
       61 actgcccgcc gtcgatgcgg gtgcaggtga cctttttgaa catatccctc acttcgcggg
      121 ctgccggcac cggggtgcgc ccggtgcgga agcatcgtga gtggactgtg tgcgggtgag
      181 tctcatgata cctggtgctg cccggatgta cgggtcggga tcacgtccgc tccgggtgaa
      241 aaaaggggga attattggtc gacagccgcg agactgacgg tgaagttccc ctgccagaag
      301 taatccaggt cgtagtctgc cgtatcggag acgaccgccg ggaactgtcg gacgaccctg
      361 aagacggcga cgtcgtcggg cagggccggg tccatcggga tgtcgacgct cttctggaag
      421 acgaggcggg caggatcaag gttcgcagcg tagtaatcct tgaacgccga ttgtctctcc
      481 tcggggatga cggtccagtc tgccgcctcg gcaaccgtcg ggtcgtttgg gacccatccg
      541 tagccgggca ggtagtactc cgcccagaag tgggtgccgg gagcctcggc catgagcatc
      601 tggtagcccc cgggagtgcg ggcggggatc ccgagcgacc ggcagagcgc tgcaaagagc
      661 atgctctggg tgccgcagtc gccgtgaccg gtctcgaaca tgtaggtgga ctcggcgacc
      721 ttgggcaccc gggcatcgag cgagtagtgc gggacatggc tgtaggggta ggtctcgatg
      781 atgtagtagt agatcatctg cgccgcaaga tgcgggttcg tctcgttccc gacgatctcc
      841 cgcgccttct cccggatggc atccgtgatc tcgatgttac gctcggatct cgtgtagagc
      901 aggtaatcgg gatcgctcgt gttgtacttg ccgaccaatg ccgggtcgat atcgccaaag
      961 atctgctcgt aggagacgaa gtcgatatcc gccgagagga tgaggtctcc gttgaccgct
     1021 ccggccggga tctcgtagta gacgtaaccg atgggccccg tggtgaacgg gccggcaacg
     1081 atgtagtccg ggcacgagag gttcgtgacg gtgacgtttc gttgtgaatc cgtctcgatc
     1141 gggagcggga accagatctt caggaccccg gacgagggga gcgcctcgcg cgggagctcg
     1201 agccgttcga cacccgcgta gcggaccgga ttcacgtagg gtccggtccc ttcgggctga
     1261 tcttccgacc aggcgtaccg ggcgatgtag ccaaagtcga gatcccttgc gttctgcttc
     1321 tgcatctcgt cgggatgcgc gtagaggtag ttcccggcga catcatcgaa gtagagcgtc
     1381 tcgttctccg agacgatcgc ctgggcgcga ttatccagcc aatcggctat cgccgcatcg
     1441 gtgatgccgg ggattgtctc cgcgagtgcc gcctctgcag ccgatctgtt gaacgggtac
     1501 tccgtatacg tcctgttcgc ccggaacatg gcgtctcgtg ctatccgcgc cctgtcaggg
     1561 tcgccggcgc cggtggagag ggcatacgcc tcggcaaaat attcttcggc agtccggtag
     1621 ctggcgtccg cgtatgcggc caccccccgg gcgtaggcct cgtcggccgg cgagatagag
     1681 gccgcgccgg gtatgttctc ttctgttgtg gcggtgcatc ccgctgcaga gaggagaagg
     1741 acggtaacta taaccagaag tgcagtgact gtgcggtagt ccataaagag aagaacatgt
     1801 gagtgctgat gaacctttct ctttttttcg gcggctttca cccggctttt ggggatcttg
     1861 tcgcgatatc ttcacaataa tccctccagc gccgactgac tgccgctttc agatcgcgcc
     1921 gggcgaagcg gccgctcccc ggccctctgc cctggtgagc gcgacggggc gcccttccat
     1981 cttgagggca gatccgcgcc tccgactgaa ggccgggatc atcgcgcatg gacgggatta
     2041 cgctggaatt atcggttaaa tcatatttca ggcagggtat gatccggcac agaatagaag
     2101 agtatatccg tgataagatg aatcacttga ttgaagcgga ggtttctatg cagagcattc
     2161 tacgttatct tttagcggtt ttccttgttg cctgctgttt cgtccttcct gcaagcgctc
     2221 agccgcctat aggcggggac aacggatggt atgcggtcaa ctgtaacgtg aacggagcac
     2281 aggtctattt cggcaatgat tataagggca cgatcgaggc cggcgtactc tacgtcccgg
     2341 tctacaccac gggaacccct taccagacgt tccgcgtcga aaaggagggt tacacgaccc
     2401 actacgggga tgtaatctcg gtcccgggca agggcgagac gtttgacctc tacgcgacct
     2461 tgaacccgat ccagcccacg caggagccgc tgatcggggg agatatcggc tggtacaccg
     2521 tccactccaa cgtcgacggc gcgacggtcc agtttgataa cgatgtcaag ggcacgatca
     2581 gcaagggtat cctcacggtg caggtctaca cgaccgggac gccgtacagg acctataccc
     2641 tgacaaagga cgggtatacg acttacaccg gttcgatcga ccgctacccc ggcaagggcg
     2701 agacggtcga cctgtacgcg acgatgaacc cggcgccgac ccccgccccg aaatcgccgc
     2761 tctccccggc ccttgccggc agcgccctgc tgatagcagg actgctgctg atcgcaggga
     2821 gaaagaacaa ctaacctctc actctttttt gcggccgctc taccctctgc tcggggatgg
     2881 agcctcgtta cctgccggat atccgcagag cacgacgtat atccccctgc tcccgcccaa
     2941 aaaaggtttg cgagaaagga accctcaggc agccccggtc ggtccctcct cctctacgga
     3001 cacccggtgg cggtcgagca ccagcccgac catcaacgcg gccagagaga gagggagggc
     3061 gatgacgaga gggtccacat cctgccaggg tgcaggaaga atcgccggca caccgaagat
     3121 gaggttggag agcccgagcg ttgccgactc cttgatgtgg acaaatgcgg tccagaggaa
     3181 ccagaccacg gctccgatga tcaggctcac ctttgcggcg aagagcgacg gcctgctgct
     3241 gtacatcgca tgggcgtatg ccggcaggaa cgccgatgcg cagaggccca tgaacatcgc
     3301 cgtcgcccgc gcgatgatgc tgccgggcat gacgaaggcg atgacgacgc tcgcgacgat
     3361 catgacgacg gtcgccacct ggatcagcct catcgatgct cctttagaac cggtgcgccg
     3421 cataacgtca aacccaagcg acgtccccat gacatggaag agggaactga gcgtggacat
     3481 cgcggcggca aggagcgcca gcatgaatat gacgacgaag aggtccggca tcgacgcgtt
     3541 gatgaagttc ggtatgatcg tgtcgacgtt gccctcggtg gcggctgcaa gagctatcac
     3601 gccctgggtc ttgtagaagt agacgttcgt gagcgcgccg accgtgaagg cgaccccggt
     3661 catcatcagg atgaacggtc cgccgacaag gaccgcacgg ttcagcgccc tgctgtcctt
     3721 gacggtcatg aaccggacga caagttgagg ctgcgcgagc acccctatgc ccacaccgag
     3781 aacgagcgtc gtcaccatgg tgagccagat cgatgagcca agcgccggca tggatgccca
     3841 cccggtcatc cctccggcgg cgagcccctg cgggacgagg tccgacatcg ctgcaagggc
     3901 ggtgtgcgcc tcgatgacgc ccccgagctg gatgtaggtg agcacgagaa ggacggtcat
     3961 cccgataagc atgatcccgc cctgcagcgc atccgtgtac atcacggcga ggagcccgcc
     4021 cagcaccacg tagagggcga cgatcgcggc gaacgcgatg agtgcggtgg tgtaggggat
     4081 ctgcagcgtg ctcgtgatga actgcgcccc gccgatcagg attgccgccg tatacagcgg
     4141 catggcgacg acgatgacga ggcctgcggc gtaccgcatg aacggcgact ggtagcactt
     4201 ccccatcagg tcggggaagg tcacggcacc gagtttactc ccgattttgc gggttttttt
     4261 cccaaatata acgaatgcta tgaatatacc gagcccgatg ttcaggacgg tcagccagat
     4321 catccccatg ccgagctgtg ctgcaacccc cccgaaaccg atgatggcgg acgtgctgat
     4381 gaaggttgct ccataggaga gggcgagaac gacaggatgc acactcctgc cggcgaccat
     4441 gtagtcgtcg tccccttttg tccggcggta accgaggtaa ccgagaccga tgacggcgac
     4501 gagatagatt agcgttattg ctatgaagag cccggtatca actgccatcc tgttcttcct
     4561 ccattccatt gttccagttc aacaggccat accccatgca tgcgagcgtg aagccaagcg
     4621 caagcagata tccgatccag attgaaggat caggaatgcc aaacataacg aaccacctgc
     4681 cggtaacggg aaaaaccgtt acattacacg ggaactcagc aaaaaggccg gttccctacc
     4741 taaatataaa gaagaatgcg ttctgcacgt gcgtgcaacg gtgggactct ctgccgttac
     4801 caatcatcga gtacctaagc aatcttctct gaattaattg tatttaaacc attctattct
     4861 ttcatccatt catgggagga ggcggcgctg ttgccggata tctcccctgg tgtgacccgg
     4921 atcggaatgc aggcccggtc aacggcggat agcctcccgc gcgggcttgc cgtggcagtc
     4981 gtcctcaggc gagggatgcc tgcggcggga gcgataccca ccggcacgcc ccctctcccg
     5041 cgccttgagc cgatacgcct tcttgcaggt tcgtttacgg ccggccgttt gtcgtgcccg
     5101 tccttgtgtg ctcagcgacc gatgcgcata actggtggga gggtataatg cactacaggg
     5161 ggcatacgcg cgggctggcg gcagagtccc gtcaggagag atgatatgga actacacctc
     5221 gttcgggatt tcggggccgg cgatatcgag gcgatcgagg tatcgcccgc agtcctggag
     5281 attgaggttg agccccgtat ggtcgatgag gctgaccgga tactcatgat gcactcggtc
     5341 ccgggaaggt ttgtctacgc cgcaggacgg tatccgggtc aattccgtgt cgagataggc
     5401 gagagtagcg accttgaccg catcggggag gcgctcctcg caattacgga gctcccgggc
     5461 agcaccccgc cttcttacgc cgtcagggat ctcatcgcgg atctctaccg gcggcgggag
     5521 gatgcgctcg agaggaaaga ggccgatact atcgaggatg agatcgcgct gggactctac
     5581 gacgacgaag actggtgatt gcggggcgat gttgccgcgc agccgggtgc cctcgccgat
     5641 aatcgcgtta ttaagtctat atcgaaaaac gaagcgcccc ggccgggatt cgaacccggg
     5701 tcgaaagctc cggaggcttt caggatatcc actaccctac cgagactgcc atatgatgtt
     5761 gtcctgtatg gtataaaagg tcagcgggag ggcgggtttg acgcccttcg cgactcgtag
     5821 agatgccaga tcttgcacgc tccctcgtgg ctgaccatgc aggggccgat gggggtgcgc
     5881 ggggtgcagg ccttcgcaaa gagccggcag tcggacggcc gggcaacgcc gcgcagcact
     5941 ttgtcgcata tgcaggccga atgtttgtcc acgtgccgga tctcgatatc gaacttcttc
     6001 tgcgcatcgt agtcctcgaa ctcaggtttc aggcgaaggc ccgaggccgg tataacggga
     6061 aagccgcgcc actcgacgtc gaacggctcg aaggcctcat acatgaggcg ttgcgccttg
     6121 acgttccctt cccgggagac cgcgcgcggg taggcgttct ccacccggtg aaccccttcg
     6181 cggacctggc gcaccgccat gaggagaccg aggaggatat cctccggctc gaaccccgcg
     6241 acgacctgcg ggacgggaaa ctgctcgtac tccttgtagc ccatcacggc acagacgtgc
     6301 ccggggagaa ggaacccctc gagcgccgcc tcgccccggg caaggagcca cgccatcgcc
     6361 ggcgggacga gccggtggca ggagaggatc gagaagttct ccggcgggcg ggagaggatc
     6421 gtggcggcga ccgtcggcgc ggtggtctcg aacccgaccg atatgaagac gacctcccgg
     6481 tcgggctccc tctctgcgat ctccaccgcc ctgtgaatgc cctgcaccac ccggacatcc
     6541 ccgccgctcg actcaagcga gcccttcgac ccggggacac gcagcaggtc gccgtaggtg
     6601 gcgacggtgc agccccgttc cacaaggtcg agcgcggcgt cgatctcgcc ctgcggcgtg
     6661 atgcagaccg ggcaccccgg tcccatcacg atcttgagcc tctcggggag gatgctccgg
     6721 agtccggcgc gggcgatcgc cgcttcgtgg gtgccgcaga tgtgcatgag cgttatgtcc
     6781 cggtcgacga gatcgaagag cgccgtgagt atatccttac caaccgccat ggatcctcat
     6841 acatatctct cgccgatagg gcatattcgt gccggtagcg ggcagacccg ggctcaggcc
     6901 gcccggtaga atacgcccct ctgctcccgc acgggctcga ccgccccctt ctcctccagg
     6961 atgcggaggt gcttcgtcac ctcggccggc cggaggccgg atatccggga gaggtcggcg
     7021 acggtgcagg ggcggcgccg gagcgtggcg agcagcatat cggcgacgtc gcgcatctcc
     7081 ggcgccgttg ccggttcgca cgatgccgcc ccgatcacct ccgcgttccc gccgatgatc
     7141 gtcctggccc gctcaagggt ccggggcgac gcccgccgga ccctgatatc ggtgccgggc
     7201 cggtcgagcg tgttcacctg gacgcggtcg ggcgcgatga cggcgacggc gtccctcagg
     7261 cggaggagct cctcatccgt atcgttcttg cccgggacga tgaatacctc aagccagatg
     7321 acgcccggaa actcccggga gaaggcgacg agacccgcga cgacctgccc ggcggtgatg
     7381 ccgggggagg ggcggttgat atcgcgaaag atatcttccg agaccgcatc gagcgacggc
     7441 acgacaaggt ccgcccgcat gagggcggcc cgaacatcgg ggtccacaaa aagcgcgccg
     7501 ttggtcagga ccgcaacacg gtagcgggga taacggtctt tgatgaacga tatgatatcg
     7561 ccgatcccgg agtgaagcgt cggttccccg gagccggcga acgtgacgta gtcgatatcc
     7621 ggcgcctttg caaggcagtc gtcgagctcc gcgatgaccc gatccgtcgg gacgtactcg
     7681 cgccgttcaa gggtgaggac ggtcgttctc ccgcactcgc agtaggcgca gtcaaacgtg
     7741 caggtcttct gcggcaccag gtcgatcccg agcgaccggc ccaggcgccg ggaggggacc
     7801 ggaccgaaga ggtaacggta tgccatgggg tgtcaccaga ctggtttggg gtgaaggttc
     7861 atcagtcttc accccggggc aacagcgggc gttctgctct catgtatcgc cgatcttgag
     7921 catcctcttg cagaactggt cgaccgatga gaagattgcc tggttgatgc cctccgggcg
     7981 ggcagggaga tcggcggtgg tggcaaaccg gagcaggtcg gagtagtaac tcagcgaagg
     8041 atacttccgg cggaactcgt cgaggcagag gttcgcctgc tccttccttc cccggagaaa
     8101 ggcgtagacg taatcgatga gacagaggac gtggcccggc atcttcttct cttccgcaag
     8161 cgttctaaac ccgttgaact ccccctcccg ggggaggtag agggtggttc ggagaccgta
     8221 gtagatgggc tcgaggtcgt cggggaaccg ctggagcata tcgttgatga ggccgagggc
     8281 atctgcaagg tcgccctggt cgaacttgag gttgtaggcc tcccggtaga agagcatgtc
     8341 gcccgggaac cggacgaggc cgatctggag cagtttcacc agcgccacgc tgttgttctc
     8401 ctgttttgcc cgtgaaagcc cgatctccag gaaccagcgg ttatccggca tccactcggt
     8461 cgcaaggaag atcatcatcc agaacggttc ggcgagccac cggtcctgct cgagctctcc
     8521 cctgaccagc cgccagagcc ccggccggag ctgcccgagt tcgtcgaggt gctggcgtgc
     8581 gagctttaag tagttccatc gctccgcggc cgagtagccg ggttcggccg acccttcctt
     8641 gtagagggta tagtagcagt aggcgtgcca gcaccccacc acggcgtcgt gcctcctgta
     8701 cggctggagg taggccgccg ccgtaagcgg catcccgatc ttcatcagct tgacggcggc
     8761 tgcaaggtgg acgccggcct tccgctcttt cttccgtttc atgccgcagg cgatctcgca
     8821 gacacggttg acgaacttca cgtctcccgc ccgtttgctg acatcaaaga cccggtatgc
     8881 gatagcgtcg atgaactcgt catgcgccgc atcggggatg gggaccccgg cacggtccag
     8941 gatcgtgacg actcttccaa agaatgaggg gaagttctcg tcgatggctt cggcgttgga
     9001 ctcgatgaag gagaagatct cggcctttga tttatcgagc tggtttgaga tgacaatctc
     9061 aggaatgaag ccatcggcca tatccaaaca tattgagggg gattagcata aatcatgcgg
     9121 atcggccacc cgtgccgcgg gtgttcggcc tctcgcggcg gccgtccgtg tcggggtgcc
     9181 gggggggttt tttccagcac gacggagcag aatgggtggg aacgagccgg aacagggtcc
     9241 ggaaggtgca tattccgaat ctatttatcg tcccggccgg aataccctgt atgtttggca
     9301 tctccaccta ctgtctccac catgaaccgc tctctctggc gctcgagagg cttgctcccc
     9361 taaccgactg cgtggaggtg atggatgagg ggctgcactt cctggagagt gcggaaccgc
     9421 tcgagagtta ctcgtacctc tacttcatcc atgcgccggc ccgggggatc aacatcgcaa
     9481 gcatccttga gcccatccgc cgggcaagcg tcgaggtcct gacgcagacg tttgccatag
     9541 ccgccgaggt gggggcggat gtggtcattc acccgggcta ctacgcgtgg agccaggagc
     9601 gggagcgggc ggcgggaagg ttccggcagt cgcttgacga gcttacggcg gccgccgccg
     9661 acctctcggt cacgttcttt gtggagaaca tggggaactg ggagtatttt ttcctgcgct
     9721 cctgcgagga cctgccgctc gtcgacggga tcgggcttgc gctcgacgtc ggccacgcgc
     9781 acctgaacgg ctgcctggac tgtttcctca cccaccctgc ggcgcatttc cacctgcacg
     9841 acaacgacgg caccgcggac acgcactccc cggtcggcgc cggaacgatc gattttactg
     9901 ccgtcatgga tgccgtcgcg aggagtaacg tcatccccat cgtcgaggtg gagaccctcg
     9961 atggggtgac cgcaagcata gatgcgctca cggcgatcga tgccgcggcg cggggcggat
    10021 agggccggcc gggcaacgat ccggcacgcc gttctcccgg catcggccgt aaccttttat
    10081 agggaaaaac tccaacatag ttggtgcgtt gtgcattagt ggctcagccg gtagagcgcg
    10141 ttcctggtaa ggacggtgcc gaaaccgcct ggttctcaca tctcggcgcc tcggtggcat
    10201 agcggtatcg cgcttccttg gtaaggaaga agtcgcgggt tcgattcccg cccgaggctt
    10261 tatattttga gttagaatct tttttccggg cgttatctca tcttttcggt atatcgggat
    10321 tgaggaccgg gcagcatata tattgctatt cccgaacacg gcgggataac tacatgggag
    10381 aaacgatccc tttttgaccg cgggggacag atcctctgga aatgagtccc acgattgacc
    10441 gtccgccgct catctacttg aacaacgccg cgacgacctg gccgaaacct ccgggagtgc
    10501 ttgcggccat cacgcaatcc cttgccctcc cggtcttcgg gtcggggagg accaccggga
    10561 gccagggcga agattacgtc acgctggcac gggaggcgat cgcaggcctc cttgccgcgg
    10621 acccgccgga gcacgtggtc ttcacatcga gcgcgaccga ctccttaaac atcctcatcc
    10681 agggtttcct cgccgggtgc gatgccggct gccacgtcct cacgaccgaa ctcgaccata
    10741 actcggtcct gcggccgctc cacgagcttg cgcggcaggg ccgcatccga ttgacggtcc
    10801 tccccttcga ggacggcgcc gtctcccccg atacggtcga ggcggccata acacccgata
    10861 cccggctcat ggtcacggcc cacgggagca acgtgctcgg gtcggtgcag gagatcgccc
    10921 gcatcgggaa gatcctgcac gatcacgaca tctccttcat cgtcgacggg gctcagactg
    10981 ccggccacat ccccgtcgac ctctggtccc tcccggtcga cgcctacgtc ttcaccgggc
    11041 ataagggcct cttcggcata ccgggcaccg gcggattcta cgtgcgggac ccggaaacaa
    11101 tcgcccccac ccgctacggc gggaccggga cggactcctt ctcgctcttt cagccccggg
    11161 atatgccgga gcggttcgag tcagggaccc acaactaccc cggccttgcc gccctcgcgg
    11221 ccggagcgga gtatatcgcg ggcatcgggg tggagacgat cgcagagaag gcagaacgcc
    11281 agacggcgtt catcatcggg gagttgaagg aagaaccgaa cgtaacggtc tacaacgagt
    11341 ccccctccct cccgatcgtc tcgttcaaca tccggggcat ggaaaacgac gacgtggggt
    11401 tcatcctcgc ccgcgcctac gggatcgtcg cccggacggg gctccactgt gcgcctctgg
    11461 cgcacagggc gatcgacggg gggagaggct ccgtgcggct cagcctctcc tggttcacga
    11521 cggacgaaga atgccgggtt gcagcccggg cgataaggga gattgcgcag catgcggatc
    11581 cttcggtcgg ttcgccataa gtcctgtgcc gacggctcct tcatgaagga gttcctcctc
    11641 gatgcgccgg tgactgcggg gttctttgcc tacctcgaga acttcgggga ggtcgaagca
    11701 ctccggaacg tcggcgaggg gttctacaag ttcgagaaga ccgactggtt ctccatcaag
    11761 ggaatgaacg gcgacaccac ggtcgaggtc aggttcaaga aggaggcgat ggacctgacg
    11821 gcagacttcg tctaccttct cttctcgtcg taccgagaag gagcggcgga cctctccctc
    11881 ctcaaacagc gtgagcaggc gatcgagaag cgggtgaaag agcgcctcta tgggccgtga
    11941 tcggaatgcc cggggatata ttcgagcagt tcgcggagga ttatgaccga tggtttgacg
    12001 agcaccgcgc cgagtaccgc gcagaactcg cccgggtccg gcgcctcctc ccccgcccgg
    12061 acgcccgcgc cgtcgaggtg ggtgccgggt ccgggcggtt tgccgcgccc ctcggcatcc
    12121 cgatcggcat cgaaccctcg ccggccctcg gcaggatggc gcgagagcga gggatcgagg
    12181 tcattcgcgg gcgggcagag tcgatcccgc tccgggacgg ggcgtgttcg tccgcgctcg
    12241 tggtgacggt catctgcttc ctggacgacc cggtcccggc gtttcgcgag attcaccgga
    12301 ttctcgcccc gcaggggact ctcgtgacag ggtttctcga acgcgaagga ccgatcgctc
    12361 agaagtacct gcacgaggag gggaagcacc ggttcctctc ccgcgcccgg ttttattcac
    12421 cggacgaagt ccgggaactc ctcgggcgca ccgggttttg tgtggccggg gctgagtcgc
    12481 aggccggttt ctccgttctt actgcgcgga aggcctgatc gagactcgcg aggaccgggt
    12541 atcgtcctcc cctcgcaaac cttatgaccg ggtacggtcg ccccccggct tctggagaca
    12601 gagaaagcgg tgcctgcacc ggatgaggcc gcggtcggcc aggagccgca tcggtgttgc
    12661 cgtcaggctc tcggtcgcga ggcttgcaga gacgatcgag ggggccaggg gcgagaacgg
    12721 atccggcagg ctccggcgct ttgcggtgtc gcggatgagg gcatacagcc agtagagggg
    12781 cgatgcgctt gagaccgcac tcatcttcat catcatgccg catccctcaa agagcgatcg
    12841 gtactgctcc agtctccggt ataccgccgc tccggtgaaa acaccctcgt gctccttgaa
    12901 ctccggttct ttcttcgcga tcatctcgat gaacgtgacg tggcctcccg gccggaccac
    12961 gcggcagatc tcctcgacgg ttcgcgcaag ctcgtcgtca tcgaggatgt agcgcaggac
    13021 ccagacggca ttgacgaggt cgaacgcgtc atcctcgaaa ggaaacggca tgccctcgat
    13081 ggtatggtgc tcggcgttct gtatgccggt attgcccgcc atccgtcgtg ctgcttccac
    13141 catccctggc gagaggtcca ctccgacaac gcgatgcacc tcctgcgcga accagagcgt
    13201 ccaccggccg accccgcacc cgtaatcgag caccgtaaaa tgcggttcca gcctgaccgc
    13261 tcttgagacg aggcgcttct ggctgccgtc gatgtacatg gtattcaggt catcgacact
    13321 gaccaccgac ctgagcggat tgccgcgatg ccgctcgttt gcttcatccc aggcatcgcg
    13381 ttgagcccgg aggtcctgcg tgagctcgcc catcttgatg cagggttgaa ccgttgccag
    13441 gcacttaacg atttctgttg tgcgatgcat gtccggccgg tatgatccta tccgactcaa
    13501 aatagtagaa agggtcagta cgcgccctta cccgtcagga ggatgcccct tgtcgggtaa
    13561 gtgccgttgc acgggccttt gaactcgtgg cgcaggctct cggagtacga attcgactgc
    13621 cagccgagga tccaccgctg gtttgttccc agaaatccgg acgagatggt gaggacgttc
    13681 tcgtcatcgg catcaaacga gagggtaaac ggcacctcca cgatgtagcc tgttgacccc
    13741 gggctcacgt agtggttttt gtcgacgaat ccgccggacc cgtcaaggtt gccgataacc
    13801 cggcagggca tgaacagggg ttcgacccct atcggcgccc gggtctctat atcgtgatcg
    13861 gggctgatac cgggcaggtg tagttccata tcaacgagca cgggcgtctc ttccgaatac
    13921 ctgtttgagt aaacatgggt cggttttggg agttccgact cgttctgccc cggcataatc
    13981 ccgattggca cgatcctgtt cgtatagacc ggatcgatcc ggtcggccga tatgttcagc
    14041 acggggatgc cgccgatata ctcgaccttt accgatatgt tctccccgtc gaagttagta
    14101 aagcttgcaa gcgacatatt gacgggggtc acgttggtcc cggagtccgg gtcgtaacgg
    14161 gaggggaggg ggatcaggag gaccgcatcc tcgatgggcc ccgacgtgga gagcgtcagg
    14221 ctgtagtggt acgacggtcg agaactcccg gagtaataca ggagccccac ggtcaacgct
    14281 gcgaatacga tcgagaatgc cgccagggcg gttgctgcga tgatgagtaa cctcttttta
    14341 cggaccaatc gagtcacctg ttatcgacca gtgatctgac tgctgctgct cttatgcctt
    14401 cctgttcttt gaaaaaccca cctccggtgc acccctgccg tgcgagccct cctgaaccgc
    14461 ggcgaactct cccctgacca caacactata taggatgacc ggtatcgggg gcccgcagaa
    14521 ggaggctaac ctatgcccta cgtaaccgtt ggtaaagaaa attcgggtaa catcgacctc
    14581 tactacgagg accatggcgc aggaagaccg gtcgtcctga tccacggctg gccgctctcg
    14641 agtaagtcct gggagaagca ggtgccggca ctgatcgatg caggctaccg cgtcgtcgcc
    14701 tacgaccgca gggggtttgg caactctgct aaaccggcat tcggctacga ctacgatacc
    14761 ctggcagaag acctgcacaa actcatgacg gagctcgacc tcacgggtgc aacgctcgtc
    14821 gggttctcga tgggcggcgg cgaggtcgcc cgctacctcg gcacct
//
LOCUS       DAOSEE010000045        14648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1632_length_14648_cov_302.038032, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000045 DAOSEE010000000
VERSION     DAOSEE010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 14648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14648
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1632_length_14648_cov_302.038032"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..1293)
                     /locus_tag="PLI76_04935"
     CDS             complement(7..1293)
                     /locus_tag="PLI76_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOI61411.1"
                     /translation="MNGRLSILLLASLIALGSIIAGCTDTSDATSASSAPPTVQTPPD
                     DAGNVTAGNTRFAFDLYRQIASAPAYTGKNIFFSPYSISSALAITYEGARGTTADEIR
                     SVLHLPANETLRRTGFAGIDADLARGDENYTLRTANALWAEKAYPFLPEYIALAERWY
                     GANTTNLDFAKNPEGSRATINRWVERETEDRIRDLLPPGSIDSMTRLVITNAIYFKGT
                     WAMQFDANQTKEEEFRVAPDKTVRIPMMRQTDEDARYPYAETDTLQVLELPYAHGDGA
                     GLSMLVLLPKNDNLTAAEEVLNAGEIAALQGSLVRKRVNVFFPKFRLETEYSLPETLA
                     AMGMPTAFSPDADLSGMDGTKDLFISGVVHKAFVDVNEEGTEAAAATGVVIGLTSARP
                     DATPIFRADHPFVFLITEKDSGTILFAGRVVNPVGP"
     gene            complement(1478..2545)
                     /locus_tag="PLI76_04940"
     CDS             complement(1478..2545)
                     /locus_tag="PLI76_04940"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOI61412.1"
                     /translation="MLFLSSQESAEWSGHAEEVLRSGTAPGTYDGDIRPNLLSAFDYY
                     IGTVLAARGRAAEGIEWLSAAALGEENDLFSAGFLLGFLERHNGRLAMPSVAFADPRP
                     FMHFAGVPMMREARKRFIAQCGTTLPQFDEPLSMMDIGCGDGGLTVSLLRHLLETGRV
                     AGIGEVLLIDSSPAMVALAEKTVRDAFPDVRIETVNSRIQDCSGSLDHRFDLAVSSLA
                     YHHMPLEQKKIHLARLKPWIDHFILFEIDANNDAPEVYSPELALSVYQSYGSIVDAVF
                     SHDAPVDVAIACVDLFLMAEVISLLTQPRGVRTDYHMLQSQWHALFTECLGPEFAPGC
                     HSPCYADEHTTLFTMHYGREA"
     gene            complement(2750..2822)
                     /locus_tag="PLI76_04945"
     tRNA            complement(2750..2822)
                     /locus_tag="PLI76_04945"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2786..2788),aa:His,seq:gtg)
     gene            3187..4968
                     /gene="infB"
                     /locus_tag="PLI76_04950"
     CDS             3187..4968
                     /gene="infB"
                     /locus_tag="PLI76_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOI61413.1"
                     /translation="MAKQSTIRTPIVCVMGHVDHGKTSLLDRIRGSSVVSTEEGAITQ
                     HIGATLVPIDAITRMSGALSKVSVNVPGLLFIDTPGHHAFTTLRARGGALADMAIVVV
                     DINEGFRPQTIEALQILRNYKTPFVIAANKIDRIHGWRAQAGQPFMKTFSQQNERVQG
                     ILETKVYELVGKLSDLGFNSERFDRVSDFARNICIVPTSALTGEGIPDILMVLIGLAQ
                     RYMTENLKVSTDGPGAGTVLEVKEERGLGMTLDLILYDGTLKVGDEIVVAGNDQIIET
                     KVRSLLKPRPMSEILLEERFERVKSVTAAAGIKVAAPRLDGVIAGSPLRAIRGGNRDE
                     AIERVRHEVQDIQVNLSDVGVIIRADTIGALEALSKELEGHQIQVMRAAVGPVTRHDV
                     IEAGTIKDPLYSAIIAFNTPVLPDAVDTLADTSMSHVSIFEGGVIYQLLDDYVEWREE
                     KKQELERQQFEKLIMPAKIRILPNCIFRQSNPAVVGVRVLGGKLQSGVDLALPSGKRV
                     GRIKQIQAKNETVQEAEAGKEVAISIDGPTVGRQINVDDDLYVDVPERHVKVIEREMI
                     NQLSASLRETLEEFTTLRRREDPFWGK"
     gene            5087..5485
                     /locus_tag="PLI76_04955"
     CDS             5087..5485
                     /locus_tag="PLI76_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6e"
                     /protein_id="HOI61414.1"
                     /translation="MADFKIILSDPGTGRSYKIDATGPAAGALIGKRIGDEIDGSVLG
                     LPGYTIRLTGGTDKTGIPARRDLPGPARRRLLLSRGVGFHPVMEGERRRKSVRGREIS
                     ADFVQINAAVAQSGAKPLAEYFSQPAAATE"
     gene            complement(5544..6542)
                     /locus_tag="PLI76_04960"
     CDS             complement(5544..6542)
                     /locus_tag="PLI76_04960"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOI61415.1"
                     /translation="MADLASCRLCEWRCGVDRLQGERGVCRTGRAEVAATMCSGTLAS
                     YIVTLLGCCYRCLHCNAYRISQYPDPGWHYAGHVPAAVLVADARERIAAYRGPRIRTI
                     GFTGGDPIIHLPYIEEVAAEVRRQGLDLGIGISTGGFATAATMARIADLCRVITLEIK
                     AWSDPVHRALTGAPVAPVLRNAEYLAREGRDRIRVFRTVVIPGMTDSEVGAIATFIAS
                     LDPGIPYRLIGFRPNNVLYYHPGPSAEEMDRLCAISREAGLDDVAWSGYYPETVPDGI
                     RALAARLVDAYGGDPAAALTAAYAAACGCPTHPRDCGACPLRGNCPATLREPWRGR"
     gene            6638..7180
                     /locus_tag="PLI76_04965"
     CDS             6638..7180
                     /locus_tag="PLI76_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOI61416.1"
                     /translation="MSMDTASPILRPVGIIRNAVQEPFLVAGRDGISQREGADASRTH
                     IRSTKEAISEIVLDESRIALLEGIEGYSHVTVLYWGHKVPEESRSVDRVHPMGRADIP
                     KTGIFSTCSPARPNPVLATVVRLHARRGNVLEVTGLDAVDGSPLIDIKPYVPSHYPQA
                     DVRVPAWMEELMKEIDERRQ"
     gene            complement(7211..7675)
                     /locus_tag="PLI76_04970"
     CDS             complement(7211..7675)
                     /locus_tag="PLI76_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2240 family protein"
                     /protein_id="HOI61417.1"
                     /translation="MSVQIAVAAPFKHMRKERLQRSEFVFYIAIDRKWMNKEQANQLL
                     ERAKSEGLIEIEGGAIRPLFDLAAISIPLGFRPTSDILAAEDPLGDLIGRIAAATGRP
                     VQDIVAEVHRVVTDHFDGNLRVEAAAVILARKYGVAFEDKLSALQRSVLKAR"
     gene            complement(7672..8094)
                     /locus_tag="PLI76_04975"
     CDS             complement(7672..8094)
                     /locus_tag="PLI76_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61418.1"
                     /translation="MRRIYHRFPQSIDLDFELVQPFRGILTCLARMHDTHTTVKEADG
                     LVKERIHIQIADGRTKFLDLDDLMPLSEKVTETADFRVSLQRTLLTPEKRLPVAENVF
                     YLRIIDKGPVTECYIAKEGRLGGLDAMDLRRILKGACE"
     gene            8185..>9582
                     /locus_tag="PLI76_04980"
     CDS             8185..>9582
                     /locus_tag="PLI76_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HOI61419.1"
                     /translation="MADHLTLLQVNDVHGYLELHPELFWTGEQARYRMAGGYARIATL
                     FEDARDERPGQVLAFDCGDTIHGTYPAVQSEGEALIPVLNTLGFNAMTAHWEFAYGPE
                     QFRKVARALDYPVLADNCYDDATGDLIFPPYAVCETDDLQVGVIGIAATIVDKVMPPS
                     FSEGIHFTLGNAELPEHIARLRDDEGVDIVVVMSHLGFPKEVKLAREVEGIDILLSGH
                     THDRLFAPAIVNDTIIIQSGCHGSFLGRLDLTVEDRRVTKFDHELIVVDEGVRPEPGV
                     EEMVEGIMEPHRERLSRVVGETRTGLNRNTAIEATMDNFLLQALLDCTGADMAFSNGW
                     RYGAPVPPGPVTVNDLWNIIPTNPPVSTVDITGRELRTMMEEDLEHTFARDPYNQMGG
                     YVKRCMGVTLYCRLENAPGLRIEDFFVGGKRLDLDAAYRAAFVTTQGVPERYGRNRED
                     LDTLAVEALERYLARG"
     assembly_gap    9583..9682
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9832..10209)
                     /locus_tag="PLI76_04985"
     CDS             complement(9832..10209)
                     /locus_tag="PLI76_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8e"
                     /protein_id="HOI61420.1"
                     /translation="MQWQGRSVRKPSGGRYHTSQGKKRSEIGRAPAETHIGEERKKVV
                     RTYGGNQKVRALRLDYATVANPATGETHKAKIEAVEANSANPNYVRRSLLTKGAVIRT
                     DVGRARIVSRPGQDGVVNAVLIA"
     gene            complement(10315..10962)
                     /gene="hypB"
                     /locus_tag="PLI76_04990"
     CDS             complement(10315..10962)
                     /gene="hypB"
                     /locus_tag="PLI76_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HOI61421.1"
                     /translation="MHHIDVHVEKDIYDVNNRIADANAEHLREHGIRAFDLLGAIGSG
                     KTATIERLVPLLRERGIRAGAIAGDVYGDDDFKRIVSLGVPACNVNTGKECHLDAHLV
                     EHAIEHLPLDDIDILFIENVGNMICPTDFRLGAEKRIVVISSTEGDDVVNKHPMMFRG
                     SNIGVINKVDLAPFVGADLDRMERDMRRYNPEMQIFRTNMKTGEGIRELLDAILA"
     gene            11017..11286
                     /locus_tag="PLI76_04995"
     CDS             11017..11286
                     /locus_tag="PLI76_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle subunit SRP19/SEC65
                     family protein"
                     /protein_id="HOI61422.1"
                     /translation="MSAERILYPCYFDGTLQRREGRRVSRNLGVKSPDLPAIETALRK
                     MKVPYRVEAHHHPAWWAEREGRVAVEWKGSKEDLIRKVAREIGRK"
     gene            11292..11936
                     /locus_tag="PLI76_05000"
     CDS             11292..11936
                     /locus_tag="PLI76_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase
                     domain-containing protein"
                     /protein_id="HOI61423.1"
                     /translation="MYDLHTHTLLSDGELLPTELVRRAAVLGYKTLAITDHADASNLV
                     HLVDAVSMVRDAARWYGVDLLVGVELTHVPPSLIPALAREAKERGADIVVVHGETVME
                     PVAPGTNRAACTCEYVDVLGHPGLITVEDARAAAEHGVALEITSRAGHNRTNGHVVGV
                     AREAGCLLTVDSDTHAPSDLMAEGARWEVALGAGLTEAESRDVLSRDVKRFLQK"
     gene            12135..12263
                     /locus_tag="PLI76_05005"
     CDS             12135..12263
                     /locus_tag="PLI76_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61424.1"
                     /translation="MDRRLKPIGKVVDIFGNISSPYAVVLCYNGCPVQVGEKIFAK"
     gene            12275..13288
                     /locus_tag="PLI76_05010"
     CDS             12275..13288
                     /locus_tag="PLI76_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription initiation factor IIB"
                     /protein_id="HOI61425.1"
                     /translation="MAEVEKLKQLQLQREALKKRGEQKVKETEKKRTEESVQSVCPEC
                     GSRQLVHDYERAELVCQSCGLVLDEEFIDRGPEWRAFDHDQRMKRSRVGAPMTFTIHD
                     KGLSTMIDWRNRDSYGRAISSKNRAQLYRLRKWQRRIRVSNATERNLAFALSELDRMA
                     SALGLPRNVRETAAVVYRDAVDKNLIRGRSIEGVAAAALYAACRQCSVPRTLDEIAEV
                     SRVSRKEIGRTYRFISRELGLKLLPTSPIDYVPRFCSGLNLKGEVQSRAVEILRQAGE
                     RELTSGRGPTGVAAAAIYISSILGGERRTQREVAEVAGVTEVTIRNRYKELAEKLDIE
                     IIL"
     gene            13301..13372
                     /locus_tag="PLI76_05015"
     tRNA            13301..13372
                     /locus_tag="PLI76_05015"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13333..13335,aa:Ala,seq:cgc)
     gene            complement(13834..>14648)
                     /locus_tag="PLI76_05020"
     CDS             complement(13834..>14648)
                     /locus_tag="PLI76_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOI61426.1"
                     /translation="STITFSKIGTIPFTLHRPYPGEVKGVLITRSGDNWYVIIQAEQE
                     VSEPKREGRSVGIDVGLDSFAVDSNGVVIENPRFYEHSRDRIKKLQQSVARKQRFSQN
                     WKKAKGKLEKAYDHITNQKNDFLHKLSRQYVDTHATICVEDLNIKYLKENGKSRGLRR
                     SIHSASWGRFFSYLSYKAASAGTRFIQVDPRNTTQMCSNCGSLVKKDLSVRVHECPYC
                     GFVADRDYNAAVNIHRVGMEQPFEPVEPRPLHHISVMQVLSMKQEAPPLRAG"
BASE COUNT         2789 a         4385 c         4501 g         2873 t
ORIGIN      
        1 cgccccttac ggccccaccg ggtttacgac cctgcctgca aagaggatcg tgccggaatc
       61 tttctccgtg atgaggaaga cgaacgggtg gtccgcccgg aagatgggcg tggcatccgg
      121 gcgtgcactc gtgaggccta tgacgacccc ggttgccgct gccgcctcgg tgccctcttc
      181 gttcacatcc acgaacgcct tatgaacgac gccgctgatg aagagatcct ttgtgccgtc
      241 catccccgag aggtcggcat cgggggagaa cgccgtcggc atccccatcg ccgcaagcgt
      301 ctcaggaagg ctgtactccg tctcgagcct gaacttcggg aagaagacgt tgacgcgctt
      361 gcggaccagc gagccctgca gggctgcgat ctctcccgca ttcaggactt cctccgcagc
      421 cgtcaggttg tcattcttcg gaagaaggac gagcatcgag agccctgctc cgtctccgtg
      481 ggcatagggg agttcaagca cctggagggt atcggtctcc gcatacggat agcgcgcatc
      541 ctcatccgtc tgccgcatca tcgggatgcg caccgtctta tcgggtgcaa cccggaactc
      601 ctcctccttc gtctggttcg catcgaactg catggcccag gtgcccttga agtagatcgc
      661 gttcgtgatg acaagccggg tcatggagtc gatcgaaccg gggggcagga ggtcgcggat
      721 ccggtcttcg gtctccctct ccacccagcg gttgatggtc gcacgcgatc cttcggggtt
      781 cttcgcgaag tcaaggttcg tcgtgtttgc cccgtaccag cgttccgcga gggcgatgta
      841 ctccgggagg aacgggtatg ccttctcggc ccagagggcg tttgccgtgc ggagggtata
      901 gttctcatcc ccgcgggcga gatcggcgtc gataccggca aatcctgttc tccggagggt
      961 ctcgttcgcc gggaggtgca gcaccgatcg gatctcgtct gcggtcgtgc cgcgggcgcc
     1021 ttcgtaggtg atcgcgaggg ccgaggagat gctgtagggc gagaagaaga tgtttttgcc
     1081 cgtgtaggcc ggggcgcttg caatctgccg gtagaggtcg aacgcgaacc gggtgttccc
     1141 ggccgtcacg ttgccggcgt cgtccggcgg agtctgcacc gtgggtggag cagaactcgc
     1201 cgatgtcgca tccgacgtgt cggtgcaccc ggcgatgatg ctgccgagcg cgatgaggga
     1261 tgcgaggagc aggatgctca gtcgtccgtt catggtctgc gatatggtgc gccgggtaat
     1321 tatatccagc gttggcgacg aattttttcg aagtcttgcg cctttcgcgc ggggtaccct
     1381 gcagcgcctg acagcgatga atcctccgcc ttcgtcaccg gagatgatct cgtaccctac
     1441 gcggcatctt cgtccgttag cagggtatag gtgctgccta cgcttcccgc ccgtaatgca
     1501 tcgtaaagag cgtcgtgtgc tcgtccgcat agcagggcga gtggcagccc ggggcgaact
     1561 ccggacccag gcattccgtg aagagcgcat gccactggct ctgcagcata tgataatcgg
     1621 tcctcacacc gcggggctgt gtgaggagcg agatgacctc cgccatcagg aagaggtcta
     1681 cgcaggcgat ggccacgtcc accggggcat cgtgcgagaa gacggcatcg acgatactcc
     1741 cgtaagactg gtagaccgag agcgcaagct ccggggaata cacctccggg gcatcgttgt
     1801 tcgcatctat ctcaaagagg atgaagtggt cgatccaggg tttgagccgc gcgaggtgga
     1861 tcttcttctg ctcaagcggc atgtggtggt aggcaagcga gctgacggcg agatcgaaac
     1921 ggtgatcgag gctgccggaa cagtcctgga tccggctgtt cacggtctcg atgcggacat
     1981 cgggaaatgc gtcgcgaacg gtcttttccg caagggcgac cattgcaggc gacgagtcga
     2041 tgaggaggac ctcaccgatc ccggcgaccc tgccggtctc taacaggtgt cgcaacagcg
     2101 atacggtgag ccctccatcg ccgcacccga tatccatcat ggagagcggt tcatcgaact
     2161 gcggcagggt ggtgccgcac tgtgcaatga accgctttcg tgcctcccgc atcatgggga
     2221 caccggcaaa gtgcatgaac gggcgcgggt cggcaaacgc cacagagggc atggcaagtc
     2281 tcccgttatg gcgctcgaga aagccaagga ggaatcccgc agagaagaga tcgttctcct
     2341 ctccaagagc agcggcgctc agccattcga tcccctctgc ggcccgtccg cgggcggcaa
     2401 gcaccgttcc tatatagtag tcgaaggcgg agagcagatt cggccggatg tcgccgtcat
     2461 aggtgcccgg cgcagtcccg ctcctgagaa cctcctctgc atgaccggac cactccgccg
     2521 actcctgcga tgaaagaaag agcatatcga ttgtattctg ccggcaaccg ttatgaggat
     2581 tcgtattctc tccgccctta cgatcccggg ggatcagagt cagctatcag cccgtagtcg
     2641 ccggtgagag cacgttagat caactgcaac ccccggttcg gcgccgtgaa tgcctctcgc
     2701 cacatgggcg aggtcagaaa gcagatcgtg aataatccga gaaagggaag gccagggccg
     2761 agattcgaac ccgggtcgag ggatccacag tcccttagga taaccgacta ccccaccctg
     2821 gcaattgtac tcataccagt ttgcggtcgg atttgattaa ggtatctctt catcgcctcg
     2881 gcaccctctc ccgcccccgg cgatcgcggg tgcatgccgg gccgggatcg ccggcgaccc
     2941 ggacctgccg cgtatcctgg cgcacggttg ccgggtctgg tgatcccggc cggagcggag
     3001 tgggtgcccg ggagaatcat taatagaccc cgtgctctat aacgtaccag tattccgggt
     3061 gccggagggc accccggggg tagggggatc atccggacca cggcatgatt caggaaaaac
     3121 ggtcaatatt ccaggttttt tggggattat ccgtccataa ctcgtatata ccggaaggtg
     3181 ataaccatgg caaagcaatc aacgatcaga acccccatcg tctgcgtgat gggtcacgtg
     3241 gaccacggca agacgtcgct tctcgacagg atccggggtt catccgtggt ctcgaccgag
     3301 gagggtgcga tcacgcagca catcggcgcc acactcgtcc ccatcgacgc gatcacccgg
     3361 atgagcggcg cgttgagcaa ggtcagcgtc aacgtccccg gcctcctctt catcgacacc
     3421 cccgggcacc atgccttcac caccctccgt gcgcgcgggg gggcgctcgc cgatatggcg
     3481 atcgtcgtgg tggatatcaa cgaggggttt cgcccgcaga cgatcgaggc gcttcagatc
     3541 ctgcgcaatt acaaaacacc gttcgtgatt gcggcaaaca agatcgaccg catccacggc
     3601 tggcgcgccc aggcaggcca accgttcatg aagacgttct cgcagcagaa cgaacgtgtc
     3661 caggggatac tcgagacgaa ggtctacgag cttgtcggca aactctccga cctcgggttc
     3721 aactccgaac ggttcgaccg ggtctccgac ttcgcgcgga acatatgcat cgtgccgacg
     3781 agcgccctca ccggcgaagg cataccggat atcctcatgg tgttgatcgg gcttgcccag
     3841 cgctacatga ccgagaacct caaggtcagc accgacggcc ccggcgccgg cactgtcctc
     3901 gaggtgaagg aggagcgggg gctcggtatg accctcgatc tgatcctcta cgacgggacc
     3961 ctcaaggtcg gggatgagat cgtcgtcgcg ggaaacgacc agatcatcga gaccaaagtg
     4021 cgctccctct taaaaccccg ccccatgagc gagatcctgc tcgaggagcg gttcgagcgg
     4081 gtcaagtccg tcaccgccgc agcggggatc aaagtcgccg ccccgaggct tgacggggtc
     4141 atcgcgggct cccccctccg ggccatccgg ggagggaacc gggacgaggc gatcgagcgg
     4201 gtccgtcacg aggttcagga catccaggtg aacctctccg atgtcggggt catcatccgg
     4261 gcggacacca tcggcgccct cgaagccctc tcgaaggagc tcgaggggca ccagatccag
     4321 gtaatgcggg ccgccgtcgg gccggtcacc cgccacgacg tcatcgaggc ggggacgatc
     4381 aaagaccccc tctacagcgc gatcatcgcc ttcaacacgc cggtcctgcc ggacgcggtc
     4441 gacaccctgg cggacacctc gatgtcccat gtcagcatat tcgagggcgg tgtcatctac
     4501 cagctcctcg acgattacgt cgagtggcga gaagaaaaga agcaggagct cgaacggcag
     4561 cagtttgaga aactgatcat gccggccaag atccggatcc tcccaaactg catcttccgg
     4621 cagagcaacc cggcggtggt cggcgtccgt gtcctcgggg ggaaactcca gagcggtgtc
     4681 gaccttgccc tcccgagcgg aaagagggtg ggccgcatca agcagataca ggcgaagaac
     4741 gagacggtcc aggaggcgga ggcgggaaaa gaggtggcga tatccatcga tggaccgaca
     4801 gtcggccggc agatcaacgt cgacgacgac ctctacgtgg acgtaccgga gcggcacgtg
     4861 aaggtgatcg agcgggagat gatcaaccag ttaagcgcga gcctgcgcga gaccctcgag
     4921 gagttcacca ccctccggcg ccgcgaagac cccttctggg ggaagtgaac cctctttgcg
     4981 cgctccggcg ggtatccccg accaccgttc cctttcaacc ccgcggaggt aggcttttaa
     5041 taccatatta tcaaattgta tagatacttt cgaaggagtc gtcaacatgg cagatttcaa
     5101 gatcatcctc tcagacccgg gtaccgggcg ttcatacaag attgatgcga ccggccctgc
     5161 cgcaggagcg ctcatcggca agcgcatcgg cgacgagatc gacgggagcg tcctcggtct
     5221 cccgggctat acgatcaggc tcaccggcgg cacggataag accggtattc ccgcacgccg
     5281 cgaccttccc gggccggcac gccggaggct cctcctctcc aggggcgtcg ggttccaccc
     5341 ggtcatggag ggagagcgtc gcagaaagtc ggtgcgtgga agggagatca gcgccgattt
     5401 cgtccagatc aacgccgccg tagcacagag cggcgcaaaa cccctggccg aatacttcag
     5461 ccagcccgca gcggcgactg aataaatcag cacccaatct tttttccgat ccgttcacag
     5521 gggccccggg aggggcagcc gttttatcgg ccgcgccagg gctcccggag ggttgccggg
     5581 caattgccgc ggagcgggca cgccccgcag tcgcgggggt gggtagggca cccgcacgcc
     5641 gcggcatatg cggcggtgag cgccgctgca gggtctcccc cgtatgcatc gacgaggcgt
     5701 gccgcgaggg ccctgatgcc gtcgggcacc gtttcggggt agtagccgct ccaggccaca
     5761 tcatcgagcc ccgcttcgcg agagatcgca cagagccgat ccatctcttc tgctgacggt
     5821 ccggggtggt agtagagaac gttgtttggc cgaaacccga tgagccggta gggtatgccc
     5881 gggtcgagcg aggcgatgaa tgttgcgatg gcgcccacct cgctatcggt catacccggg
     5941 atcaccaccg ttcggaagac ccggatccgg tctcttccct cgcgcgcgag gtattctgcg
     6001 ttcctgagca ccggggccac cggcgctccc gtgagggcgc ggtggaccgg gtccgaccat
     6061 gccttgatct cgagggtgat cacccggcag aggtcggcaa tccgcgccat cgtcgccgcc
     6121 gtcgcaaacc cgccggtcga tatccctatg ccgagatcga gaccctgtcg cctcacctcg
     6181 gccgcgacct cctcgatgta ggggaggtgg atgatcggat ccccgcccgt aaacccgatc
     6241 gtccgtatgc ggggcccccg gtacgcggca atccgctccc gggcatcggc caccagcacc
     6301 gccgcaggca cgtggccggc gtagtgccag ccgggatcgg ggtactgtga tatccggtag
     6361 gcgttgcagt ggaggcagcg gtaacagcac ccgagcaggg tgacgatgta acttgcgaga
     6421 gtgcccgaac acatcgtcgc cgccacctct gcgcggccgg tgcggcatac cccccgctcc
     6481 ccttgcaggc ggtcgactcc gcaccgccac tcgcagagac gacaggacgc aagatctgcc
     6541 atactcttct ctggtgtctg catgcaccat aatctctggg aatccggcag gagccatgcc
     6601 gccaccggtg caactattcg cgagcggatg ccagagtatg agcatggata ccgcatcacc
     6661 gatcctgcgg cccgtgggga taatccgtaa cgccgtgcag gaaccgttcc tcgtcgccgg
     6721 cagggacggc atctcacagc gggaaggggc cgacgcgagc aggacgcata tccggagcac
     6781 gaaagaggcg atatcggaga ttgtcctcga tgagagccgt atcgcgctgc ttgaaggtat
     6841 cgagggctac tcccacgtca cggtgctcta ctgggggcac aaagtgccgg aggagagccg
     6901 gtccgtcgac cgcgtgcacc cgatgggcag ggcggacata ccgaagacgg ggatcttctc
     6961 gacctgcagt cctgcccgcc cgaaccccgt gctcgcgacc gtcgtccggc tgcatgcgcg
     7021 gcgcgggaac gtactcgagg ttacgggtct cgatgccgtc gacggcagcc cgctcatcga
     7081 tatcaaaccc tacgtcccgt cccattaccc acaggccgat gtgcgggttc cggcatggat
     7141 ggaggagctg atgaaagaga tagacgagcg gcggcagtaa acctgcgaag aggggcggtc
     7201 gaatgaagcc ttaccgcgct ttcaggactg atcgctggag ggcggacagt ttatcctcga
     7261 acgccacccc gtattttctt gcgaggatga ccgccgccgc ctccacccgg aggttgccgt
     7321 cgaagtggtc ggtgacgacc cggtgaacct cagcaacgat atcctgcacc ggtctcccgg
     7381 tcgcagcggc gatgcgaccg atgagatctc ccagggggtc ctccgcggcg agtatatcgg
     7441 atgtgggtct gaacccgagc ggtatcgaga tcgccgcgag atcgaagagc ggtcggatgg
     7501 cgcctccctc tatctcgatg agcccttccg acttcgcccg ctctagaagc tggtttgcct
     7561 gctctttgtt catccacttc cggtcgatgg cgatatagaa gacgaactcg ctcctctgca
     7621 atcgctcctt gcgcatatgt ttgaacggtg cggcgaccgc aatctgcacg ctcactcgca
     7681 ggcacccttg aggattctcc gcaggtccat cgcgtccagg ccgcccagtc gtccctcttt
     7741 tgcgatgtag cactctgtca ccggcccttt atcgatgatc ctgagataaa agacgttctc
     7801 tgcaaccggc aacctcttct cgggggtgag gagcgttctc tgcaggctga cgcgaaagtc
     7861 tgccgtttcg gtgactttct cggagagcgg catcaggtcg tcgaggtcca ggaactttgt
     7921 ccgcccatcc gcgatctgga tgtggatccg ttccttgacg aggccgtctg cctccttcac
     7981 tgtcgtgtgg gtgtcgtgca tccgggccag gcaggtgagg atgcctcgaa atggttggac
     8041 aagttcaaaa tcgaggtcga tggactgggg gaaacgatgg tatatcctgc gcatcactag
     8101 aattgatacc cgcagaacgt aaaaagcatc cgatcccgcc ggagacacaa agatttttgg
     8161 cacacccgcc cctcccccgc acccatggcg gatcatctta cgctcctgca ggtgaacgac
     8221 gtgcacggct acctggaact gcaccccgag ctcttctgga ccggcgaaca ggcccggtac
     8281 cggatggccg gggggtatgc caggattgcc actctcttcg aagatgcccg cgatgagcga
     8341 ccgggtcagg tgcttgcgtt tgactgcggc gacaccatcc acgggaccta cccggccgtc
     8401 caatcggagg gtgaggcgct catcccggtc ctcaacaccc tcgggtttaa cgctatgacc
     8461 gcgcactggg agtttgccta cgggccggag cagttccgga aggtcgcccg cgctcttgac
     8521 tacccggtgc tcgccgataa ctgctacgac gacgcaacgg gcgatctcat ctttccccct
     8581 tacgctgtct gcgagaccga cgatctccag gtgggtgtca tcgggatagc ggccacgatc
     8641 gtcgataagg tgatgccgcc gtctttctcg gagggtatcc acttcaccct ggggaacgcc
     8701 gaactcccgg agcatatcgc ccggcttcgc gacgatgagg gagtggatat cgtcgtggtg
     8761 atgtcgcacc tcggttttcc caaggaggtg aagcttgccc gggaggtgga gggtatcgat
     8821 atcctcctct cggggcacac ccacgaccgc ctctttgcgc cggccatcgt caacgatacc
     8881 atcatcatac agtccggttg ccacggctcc ttcctcgggc ggctcgatct tacggtcgag
     8941 gaccggcggg tgacgaagtt cgatcacgaa ctcatcgtcg tcgatgaggg agtccggccc
     9001 gagcccgggg tcgaggagat ggtcgagggt atcatggaac cccaccgcga acgcctctcc
     9061 cgggtcgtcg gggagacgcg gacggggctc aaccggaata cggctatcga ggccacgatg
     9121 gacaacttcc tcctccaggc gctcctcgac tgcaccggcg cggatatggc gttctcaaac
     9181 ggctggcgct acggcgcccc ggtgccgccg ggtccggtca cggtaaacga cctctggaac
     9241 atcatcccga caaacccccc ggtatcgacg gtcgatatca cgggccggga actccggacg
     9301 atgatggagg aggacctgga gcatacattt gcgcgggacc cttacaacca gatgggcggc
     9361 tacgtgaagc gctgtatggg ggtcaccctc tactgcagac tcgagaacgc gcccgggctc
     9421 cgcatcgagg atttctttgt cgggggaaag aggcttgacc tggatgccgc ctaccgggcc
     9481 gcgttcgtca ccacccaggg cgtgccggag cgttatggga ggaaccggga agatctcgat
     9541 accctcgcgg tcgaggcgct cgagcgttac ctggcaagag ggnnnnnnnn nnnnnnnnnn
     9601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn nnggaagatc tcgataccct cgcggtcgag gcgctcgagc
     9721 gttacctggc aagagggccc gtgagcgccg atctctacgg gagcgtggtg gcgatatgat
     9781 gccgtgaccc gatcgccggt gcggtgggct ctgtgcccac aaaaaagtat cttatgcgat
     9841 caggacagcg ttgacgacgc cgtcctgacc gggtctgctg acgatccgtg cgcgcccaac
     9901 atccgtcctg atgacggcgc ctttggtcag gaggctccgc cggacgtagt tcgggttggc
     9961 gctgtttgcc tcgaccgcct cgatcttcgc cttgtgggtc tcgccggttg ccgggtttgc
    10021 gaccgttgcg tagtccaggc ggagcgcccg aaccttctgg ttgcccccgt aggtccggac
    10081 gaccttcttg cgctcttcac cgatatgcgt ctctgccgga gctctgccga tctccgatct
    10141 cttcttgccc tgggaggtgt ggtagcgtcc gcccgatggc ttccgtactg atcttccttg
    10201 ccactgcatg tgaccaccga tatactctga tgaggacgcg atacccgtcg cgggaaagca
    10261 atctctcgtt aaagtgctat aactattcgg ggcgggaata tttaactgtg ctgatcacgc
    10321 gaggatcgcg tccagcagtt cccggatccc ttccccggtc ttcatgttcg tccggaagat
    10381 ctgcatctcc gggttgtagc ggcgcatgtc gcgttccatc cggtcgaggt cggcgccgac
    10441 aaacggagcg aggtcgacct tgttgatgac gccgatgtta ctgccccgaa acatcatcgg
    10501 gtgcttgttc accacatcgt ccccctccgt cgagctgatg acgacgatcc tcttctcggc
    10561 gcccagccgg aagtcggtcg ggcagatcat gttgccgacg ttctcgatga agaggatgtc
    10621 gatatcgtca agcggcaggt gttctatggc gtgctccacc aggtgggcgt cgaggtgaca
    10681 ctctttcccg gtgttcacgt tgcaggccgg aaccccgaga gaaacgatcc gcttaaaatc
    10741 atcgtcaccg tagacgtctc ctgcgatggc gccggcgcgg atcccccgct ccctaagaag
    10801 cgggacgagt cgctcgattg tggcggtctt ccccgaccca atcgcgccga ggaggtcgaa
    10861 cgcccggatg ccgtgctccc tgagatgttc ggcgttggcg tccgcgatgc ggttgttgac
    10921 gtcgtagatg tccttctcca catggacgtc gatatggtgc atggtaagta atgtgatcat
    10981 gatctgttta taggttaaca cccaacccct acggcaatga gcgctgaacg catcctgtat
    11041 ccctgttact tcgacggcac gctgcagcgg cgggagggcc gccgggtctc gagaaacctc
    11101 ggcgtgaagt cgccggatct ccccgccatc gagaccgcct tgcggaagat gaaggttccg
    11161 taccgggtgg aggcgcacca ccaccccgcc tggtgggcgg agcgcgaagg ccgggttgcg
    11221 gtggagtgga aagggagtaa ggaagacctg attcggaagg ttgcccgcga gattggccgg
    11281 aagtgacccc gatgtacgac ctgcacactc acaccctcct ctccgacggc gaactcctcc
    11341 cgacggaact cgtccggcgg gctgccgtcc tcggctataa gactctcgcc atcaccgacc
    11401 atgcggatgc ctcgaacctc gtgcatctgg tggatgcggt gagcatggtc cgggatgcgg
    11461 cgcgatggta cggcgtggac ctgctcgtcg gtgtggaact cacccatgtc ccgccgtcct
    11521 tgatcccggc gctcgcgcgc gaggcgaaag agcgcggtgc cgatatcgtc gtggtccatg
    11581 gggagacggt gatggagccg gtcgcgcccg ggaccaaccg cgccgcctgc acctgcgagt
    11641 acgtcgacgt gctcggccac cccgggctga taacggtcga ggatgcccgc gcggctgcag
    11701 agcacggtgt cgcgcttgag atcacctccc gtgcggggca taaccggaca aacggccatg
    11761 tggtgggtgt ggcacgggag gccggctgtc tcctcacggt cgattccgat acgcatgcac
    11821 cgtccgatct gatggcagag ggggcgcggt gggaggttgc gctcggcgcg gggctgacgg
    11881 aggcggaatc acgggatgta ctctcccggg atgtgaaacg gtttcttcag aagtaagatg
    11941 gagtatttat attttagcga aacgttttta taatcttatg ttcaatatat atacgttact
    12001 aaatatatcc agctagagtg ttccctagag gttgcagttt gcggttaatc ggtcgttctg
    12061 taagtgtttg cggtaatcgc ttgttaatct tgcgatgtga tgctgcgcag ttgccccgcc
    12121 tctacggtga ggcagtggat cgccggttaa aacccatagg gaaggttgtc gatatctttg
    12181 ggaatatatc gtctccctat gccgtagtcc tctgctacaa cggctgtccc gtgcaggttg
    12241 gcgagaagat ctttgcaaaa taggtgtata atcgatggca gaagttgaaa aattaaagca
    12301 gctgcagttg cagcgtgagg ccctgaagaa gagaggggag cagaaggtta aagagaccga
    12361 gaagaagcgc accgaggaga gtgtccagtc cgtctgtccc gagtgcggca gccgccagct
    12421 cgtccacgat tacgagcgtg ccgaactcgt atgccagagc tgcgggcttg tcctcgatga
    12481 agagttcatc gatcgcggtc cggagtggcg tgcattcgac cacgatcagc ggatgaagcg
    12541 ctcccgtgtc ggcgcaccga tgacgttcac gatccacgac aagggtctct cgacgatgat
    12601 cgactggagg aaccgtgact cctacggtcg tgcgatctcg agcaagaacc gtgcccagct
    12661 ctaccggctc cggaagtggc agcggcgtat tcgtgtctcg aacgcgaccg agcggaacct
    12721 cgcgttcgct ctctccgagc tggaccggat ggcgtctgcg ctcggcctgc cccggaacgt
    12781 gcgcgagacc gccgcggtgg tctaccgtga tgcggtggac aagaacctga tccgcggccg
    12841 gagtatcgag ggtgttgccg cggcggcgct gtatgcggcg tgccggcagt gcagcgtgcc
    12901 ccggacgctc gacgagatcg cggaggtctc ccgtgtatcg aggaaggaga tcgggcgcac
    12961 ctaccggttc atatcgcgcg agctcgggtt gaagcttctg ccgacgtccc cgatcgacta
    13021 cgttccgcgc ttctgctcgg gcctgaacct gaagggtgag gtccagagcc gggcggtcga
    13081 gatcctccgg caggccggag aacgcgaact tacgagcggc agagggccaa cgggcgtcgc
    13141 tgctgccgcc atttacatct cctcgatcct cggcggagag cggcgcaccc agcgcgaagt
    13201 tgccgaggtt gcgggcgtga ccgaggtcac gatcaggaac agatataagg aactggcaga
    13261 aaaattagat atcgagatca tactctgatc tcctatcttt gggctcgtag atcaggggta
    13321 gatcgctacg ttcgcaacgt agaggccgcg ggttcaaatc ccgccgagtc catttttctg
    13381 ttttcacact gtgttcttca tgtaactttt actgcgttct tcaagaccta tgggcgacca
    13441 gaggtcgcca ggggttttga gcggggcgca gcccctttcg gtccgcaaaa ggaaaggcca
    13501 cgggttcaaa tcccgccggg tccgccctcc tgttcactta tcacactctg caggcaatct
    13561 acttctcctt tctaccgtcc tgacggttat gtgtcttccc ccgcactctc gggcacctta
    13621 ttttccggac ggagttctta atggtgtaat ggctaaaaat ggttattgtg aaagcgcatg
    13681 aaaatgtaaa tggttatatg ttgttaacaa gcattcagat caatggatag cgaattaatc
    13741 tcaatgatac ctattatttt gccatttgca ggccttattg cactcatcat accgttgatt
    13801 ggtatgattg tcaggctatc agaaaggggt gaactacccc gcccttaagg gcggggcttc
    13861 ctgcttcatg gacaatactt gcatcaccga gatgtgatgt agaggtcttg gctccacagg
    13921 ctcgaagggc tgttccatcc ccacgcggtg aatattcacc gcagcattgt agtctcgatc
    13981 ggcaacaaac ccgcaatagg ggcattcgtg aactctcacc gagagatcct tcttcacgag
    14041 acttccgcag ttcgagcaca tctgcgtcgt attccgggga tcgacttgaa tgaacctcgt
    14101 accagcactt gcagccttgt acgaaagata cgaaaagaat cgcccccacg acgcactgtg
    14161 gatacttctc cggagtccac gagatttgcc gttctctttc aggtacttga tattcaggtc
    14221 ttcaacacag atcgttgcat gggtgtcaac gtactgacga gaaagtttgt gcaggaaatc
    14281 gttcttctgg ttcgtgatat ggtcatatgc cttctccagt ttcccttttg ctttcttcca
    14341 gttctgcgaa aaccgctgct ttcgagcaac gctctgctgc aacttcttga tccggtcacg
    14401 tgagtgttca tagaaccggg ggttctcgat tactacaccg ttgctatcga ccgcaaacga
    14461 atccaacccg acgtcgatgc cgacggatcg cccctccctc ttcggttcgg aaacttcctg
    14521 ttccgcctgg atgatgacgt accagttgtc accggaacgg gtgatcagga cacctttcac
    14581 ctctcccggg tatggtcgat gcagggtgaa cgggattgtt ccgatcttcg agaacgtgat
    14641 cgtgctgt
//
LOCUS       DAOSEE010000046        14555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1645_length_14555_cov_222.252207, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000046 DAOSEE010000000
VERSION     DAOSEE010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 14555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14555
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1645_length_14555_cov_222.252207"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(205..1614)
                     /locus_tag="PLI76_05025"
     CDS             complement(205..1614)
                     /locus_tag="PLI76_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HOI61427.1"
                     /translation="MPRIYLGKIPLRWCDTCHTPVLSGTCACGAPARPVAVTPPGDAR
                     PAFPDDVDRVNRVFCEHFGAPLIPEGHLSLLNKVPADDRMDEIVLGGAVVGAIRYIPG
                     EQRWEPLPRPNAAAFLRPTKRYVVVDDGAIPSIRDGGASVLAPGLLSIDPAVAEGDEV
                     FILTEAGECIGVGRAKVDAAVATTMERGLVVRTRRNVSSACVPGEATWEDAVAANERI
                     LAEYEAASIQFVRDVAEKNPERPTVSYSGGKDSLATLLIVLKALGPVPLLFADTGLEF
                     PETCENVEAVAERYGLEVLRVCDEAAFWSEFERSGPPAVDNRWCCRVCKLHPLGRLID
                     EHFGECLSFIGQRKYESVRRMQSRRVWRNDRVPQQLSAAPIQQWTAMHVWLYIFREKA
                     PYNRLYERGLDRIGCFMCPSSDLATFALIRESCPELSEMWLAKLAGWQEKQGLPRDWV
                     ERGLWRKRGGADEEEDSYN"
     gene            2180..2638
                     /locus_tag="PLI76_05030"
     CDS             2180..2638
                     /locus_tag="PLI76_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HOI61428.1"
                     /translation="MHEGRIAGVALGAAFALAVGLYAASGHLAPGWAPPGCAVLAIGV
                     VLPFFTSGAEVPSVRVARKPMRKIEILGLLIFILMAVVMLAVGAALFCDWLAEPGSAP
                     VGTPVELGPVSGELGVLGTIPVMALMIGVEVVGGFALIALYMLSAVGREG"
     assembly_gap    2917..3016
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3286..3873)
                     /locus_tag="PLI76_05035"
     CDS             complement(3286..3873)
                     /locus_tag="PLI76_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HOI61429.1"
                     /translation="MDFLSAGALTPILIPLLVFGARVIDVSLETLRIILLARGGRLIV
                     PAIAFVEIIFWIVSLGLVVNEFTNPIYLISYGAGFAAGNYAGILLEERLAMGICVLRV
                     IASEEDHALIDAIRGTGYGVTVVAAEGLHGPCTIFYSVVRRCDLFPITRMVEEMNPGA
                     FCTVEDVRSTTHGTFPRAGERTLSGIPRRTVRLGK"
     gene            complement(4002..6005)
                     /locus_tag="PLI76_05040"
     CDS             complement(4002..6005)
                     /locus_tag="PLI76_05040"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOI61430.1"
                     /translation="MDTAFLEQILIIFALSIAALALCYRMKMPGIVAFFLTGIIAGPY
                     GLGLITNRADVEILAELGIIFLLFTIGMELSLKSLLDMKKGMLTGGVLQIGLTIGVVA
                     VIASAFGLAPPEAIFFGMLLAHTSTTVMLTVFQHRGEVDTPPVRLALGISVLQDLSTV
                     PMILLVPMLGGMGSAGVVPSLINFALGLVLLAAVTASALWVVPRFLYRVASLRSRELF
                     MITVITLCLGTAWLTSRAGLSLALGAFLAGLIISESEYSNAALSTVIPFRDVFTSFFF
                     VSMGMLLNTGFFAAHALLIVAIIIGVIVLKAIIGAVAVLPAGVSLRTAVLTGLAIGQI
                     GEFAFILSRTGIEHGLLSPDLEQIFLAVSVGTMAVAPFVIASAPQATAVVRRLPLPVW
                     LRARDASLKVAPANGPGEGHIVIVGFGPIGRHVAEAARSTGVPYMAIELNPKTVREER
                     KKGEPIFFGDAINEGVLVHAGVERARVVVITIPDAATARQVTGIARKLNPDCAIITRT
                     RYMENSLALYAIGANDVVCEEFETAAKVLSRVLVRYPVQKSDIDRLVASMRTGNYEML
                     RRASGQVPLLHDIGLHLDNAEVSIVRVDEGAPIAGKTLAEADLRRRYAVTVLAVRRGT
                     ETLAGPDGGTVIEAGDLCILVEAGPGNPDIASLFRHGTEGQGS"
     gene            complement(6229..7395)
                     /locus_tag="PLI76_05045"
     CDS             complement(6229..7395)
                     /locus_tag="PLI76_05045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOI61431.1"
                     /translation="MVVTPYFYPHMGGVQKYVYEIGTRLQRAFGYDLTIVSSNWHKEE
                     NTAKTEEIGGLEVVRLPYQFKVSSTPVSLGWERALRDLIRSERPDVVNGHMPVPFIAD
                     AAARAAAGMQVPYVLTYHNDLTGHDLPTRIIGRCYYPLLGSRTLHLSSAIVATSDYYA
                     QQSSHLAGYLEKLRYAAPGVDLERFGGDRTDFLRRKYRLGDEKILLYVGHLDRESRHK
                     GVQYLIRAMRIVRESLDAKLVVAGRGNYIDRYRRLAADEGVPEDIIFAGFIPEDEMPL
                     YFNSADLLVLPSCDRAEGFGMVLIEAQACGTPVIGTDVGGIPFAVDDGVTGLIVPPGD
                     VSGLAGAIVRVLDDPDLYRAMAENGPRRVRDLFTWEKSARVYDRVFCEAAGERA"
     gene            7959..9131
                     /locus_tag="PLI76_05050"
     CDS             7959..9131
                     /locus_tag="PLI76_05050"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOI61432.1"
                     /translation="MSRGAVFVTDAQMRSSLAVIRSLGKQGLDVTAGEETRCATGVFS
                     RYCRRGLVYPSPKKAEERFIGYLLDTLRSGEIDVLFPVADACLKPIVDHEEEISRYTR
                     IALPPRDIFTAGYDKGTTLRIAMENGIPCPRTFFPESPDDAAGIAEKIDYPAVVKPRV
                     SSGRRGVRVCRDSQDLVQAYAASASEFGPVVIQEFIPYGGELGVYTLLNAQSEPRAVT
                     VQRRIRSYPISGGASTLRETIKDERSREAVGIALRLLKAMRWSGVAMVEFRVDARDGS
                     LKLMEVNPRFWGSLNLSILSGVDFPYLLYRMVMDGDIEPVLDYQEGVLCRWLLPGDIL
                     WYLSAPKTWENLKEFLRFDTPDDILSLHDPGPTLGFALATARYALDPDMWRFVLRR"
     gene            9133..10155
                     /locus_tag="PLI76_05055"
     CDS             9133..10155
                     /locus_tag="PLI76_05055"
                     /inference="COORDINATES: protein motif:HMM:NF023385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOI61433.1"
                     /translation="MAVNALSIDLEFWWCNEFLNGYLPGDREDMFKESLRPIFASLDA
                     FNVRATFFVLGAVAERYPEVIHDIHRRGHEIACHAYSHKPLYSMDQREFERELGKSLE
                     LLADYNPVGFRAPSFSLSNRTKWALESLEKYGFQYDSSIFPVRTPLYGAPDAPVGIYR
                     PSRDDITVHDPDGPIIEFPLSIVRFGVNIPISGGFYLRSLPKQVLAWGIGRVAAKRPA
                     NIYLHPHDIYPGPPPLKVPFLSRLVTYHGVHTSLEKLIMLMMKFSFMPIGALLDEMSL
                     LSPEAPVPMAPARPLRGVGGRDDPAVPTIPAPTLQLQPFDGVRQRYPARAARPIHETG
                     DPDETL"
     gene            10335..11324
                     /locus_tag="PLI76_05060"
     CDS             10335..11324
                     /locus_tag="PLI76_05060"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOI61434.1"
                     /translation="MDWTEKRVLVTGAGGFIGSHLTESLVDHGADVRAFVRYNSRNDP
                     GLLRLLPPERLDRVRILMGDLRDADAVRRAVRDVDVVFHLGSLIAIPYSYARPRETVE
                     TNVIGALNVFEAARDAGVEKVVHTSTSEVYGTARYVPIDENHPLQGQSPYSASKIGAD
                     KVAESFYRSFDVPIATIRPFNTYGPRQSARAVIPTIITQALVQGKVALGATHTTRDYT
                     YVDDLVRAFLLIAESPGTVGETINVGSNYEISIGAVAGTVAALLGKEIDIITESERLR
                     PEKSEVERLWCDNTRARDLLGWSPAVPLEEGLLRTIAWFREHLGLYRPNHYVT"
     gene            11326..12042
                     /locus_tag="PLI76_05065"
     CDS             11326..12042
                     /locus_tag="PLI76_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOI61435.1"
                     /translation="MKAIILAGGKGRRLEPYTTIIPKPLMPIGDKPILEIILCQLREH
                     GIRDVTIAVGHLAELIMAFFGDGSRFGLSITYSREERPLGTAGCLGLIKDDLIEPFLM
                     MNGDVLTTLPFSELMDFHQKSGAIATVALNKRDYFVDFGIVDTNGNGDIVGYTEKPGF
                     EHYVSMGVYAFDPRVLAYITPHEYIDFPDLIKALLAAGETVKAYKYGGYWLDIGRVDD
                     YKKANNEIAGLYGTLGVGGE"
     gene            12044..13213
                     /locus_tag="PLI76_05070"
     CDS             12044..13213
                     /locus_tag="PLI76_05070"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOI61436.1"
                     /translation="MAWKIPLSDIAMDGEEVDAVRDVLQSRWLSMGPVTEEFEAAFAR
                     YLGAEHAFAVSNGTAALHIAHAVLGIGSGDEVITPSLTFVATANSVLYCGARPVFADI
                     AGPDDLNVAWEEIAAKITPETRAITVVHYGGYPCDMQPILDLARDHDLRVVEDAAHAV
                     GATYRGQKCGTIGDVGCFSFFANKNLATGEGGMVVTDDDDLAERIRVMRSHGMTTLTW
                     DRHRGHGYSYDVTAIGYNYRTSEVASALGIVQLRRLAENNARRKRIAGRYRKLLEGVE
                     GIECPFAGAVDKESSHHIFPVVLSQDLSRAGLQEALKEKGIQTSIHYPPIHLFTYYRG
                     LFGDTRGSLPKTEFAGEHEMTLPLYPGMTDDEVEYVCTAIAGAAPGILAGGRGAA"
     gene            13210..13845
                     /locus_tag="PLI76_05075"
     CDS             13210..13845
                     /locus_tag="PLI76_05075"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOI61437.1"
                     /translation="MIADLHIHTRYSHDSFMDPARVVRVARMRGLSAIAVTDHNTIRG
                     GLLAREANPGGDFEVIVGAEIRTEYGDVLGLFLDREIAARRFDDVVEEIHAQGGLAVL
                     AHPYRRYPSPERLAGRVDLIEGFNSRSRRQANALSMRFGRASGKGTVGGSDAHTYAEI
                     GRGVTIYAGSEIEKALRAGQTAGEGRESNYYVVHGLSCGIERLKRLVPGRG"
     gene            13985..>14555
                     /locus_tag="PLI76_05080"
     CDS             13985..>14555
                     /locus_tag="PLI76_05080"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOI61438.1"
                     /translation="MLARSRAYLNVEQIVDDLSREDLEDESHIRTFEDRFCSYIGAER
                     CHATNQARAALLIALRALDLSPGDEVLVPDFTFEGVVDAILESGATPVFVEPALQDFN
                     VPASHFIEKMTERTRAVIATHLFGIPCDIGDLPAIAKENDCVVIEDCAQCLGAEYNGR
                     PVGTFGDFTIVSFNYEKHLTTGEGGMLAVN"
BASE COUNT         2691 a         4520 c         4576 g         2668 t
ORIGIN      
        1 gctaccttac ttctcgtcgc ccgcgccttc ggcgctccgc ccctaccgcg gggatggccg
       61 ggggcagtgc ccgggtgttg tcggcgggga gccgtgctga aaagagatat ggcgccggcg
      121 acgggaaggg ggtcgtcacc ggatccatcg tgattgccgc gaccgcccct gcctgttcaa
      181 ccccggaggc tgccgggccc gcaatcaatt ataactatcc tcttcctcgt cggcgcctcc
      241 ccgcttgcgc cacaacccgc gctcgaccca gtcacggggg aggccctgct tctcctgcca
      301 tccggcaagt tttgcgagcc acatctcaga gagttcgggg caggactccc tgatgagcgc
      361 aaacgtcgca aggtcgcttg aagggcacat gaaacatccg atgcggtcga gcccgcgctc
      421 gtagaggcgg ttgtaggggg ctttctcgcg gaagatgtag agccagacgt gcatcgcggt
      481 ccactgctgg atgggggccg ccgagagttg ctgcgggaca cggtcgttcc gccagacccg
      541 cctgctctgc atccgccgca ccgactcgta cttccgctgc ccgatgaacg agaggcactc
      601 tccaaagtgt tcgtcgatca ggcggccgag ggggtggagc ttgcagaccc ggcaacacca
      661 gcggttatcg acggccggcg ggccgctcct ctcaaactcg ctccagaacg ctgcctcatc
      721 gcatacccgg aggacctcaa gcccatagcg ttctgccacc gcctccacgt tctcgcaggt
      781 ctcggggaac tcaagccccg tatcggcaaa gaggagcgga accgggccga gcgcttttaa
      841 gacgataagc agggttgcga ggctgtcttt cccgcccgag taggagaccg tgggccgctc
      901 cgggttcttc tccgcaacat cccggacaaa ctggatgctt gcggcctcgt actccgcaag
      961 gatgcgttcg tttgccgcaa cggcatcttc ccacgtcgcc tcgcccggga cacaggctga
     1021 tgaaacgttc ctgcgcgtcc ggacgacgag cccccgctcc atcgtcgtgg cgacggcggc
     1081 gtcgaccttc gccctgccga caccgatgca ttcgccggct tctgtcagta tgaagacctc
     1141 atcgccttcg gcgacggcag gatcgatcga gaggaggccg ggtgcgagga cgcttgcccc
     1201 tccgtcacgg atggatggga tcgccccatc gtcgacgacg acgtagcgtt tcgtcggccg
     1261 caggaaggca gcggcgttcg gacgcgggag gggctcccag cgctgttctc cgggaatata
     1321 gcggatcgcg cccacgaccg cgccgccaag gacaatctcg tccatgcgat cgtcggcggg
     1381 aaccttgttg aggagcgaca ggtgcccttc cggtatcagc ggcgctccga aatgctcgca
     1441 gaatacgcgg ttgacccgat cgacgtcatc agggaacgcg gggcgggcat ctcccggtgg
     1501 cgtgacggct accgggcggg ctggcgctcc gcaggcacag gttcccgaga gcaccggggt
     1561 atggcaggta tcgcaccagc gaagcgggat ctttccgagg taaatgcgag gcattcttat
     1621 acccattggg gagaaataga taaaagtctt ggcgcacttc cgatggtttt cgcaatctcc
     1681 ctccggagag tattctgctg gagtctgccg gcgacggccc tttatcaagg caggaatcgc
     1741 tctcgcctta cgacgatctc ctgttgccgg tattggaggg tcagggcata tcattaaata
     1801 tatcctctgc ctcatagtat tatcatgttc aatcatgtag gatcgtgctc gccgccttct
     1861 tgccggcccg ccgacgattc tgcctgatga aaggagagat accgtatgta ccgggtgatg
     1921 ttcacgaccc ccttcccgct gaccgttcag gtcgcaggtg cagtgtctgg ctttcccttc
     1981 gcaacgaccg gttatccggt caagcgggaa gccacaacta ccgggaggca gggtccgcgt
     2041 gccgggttgt ccgcggagcc tgcaccttcg gttcgttctg catggaaggc cactctcgtg
     2101 aagcgcccgg cggccggttt ctcggccgca tctctcgtat tcggccagct cttcggagat
     2161 ggttcccgga gtgtgccgca tgcatgaggg ccgaatagcc ggcgtagccc tcggagccgc
     2221 cttcgccctg gcggtcgggc tctatgcggc gagtggccac cttgcgcccg ggtgggcgcc
     2281 ccccgggtgt gcggtgcttg cgatcggggt tgtcctgccg ttcttcacct cgggcgcgga
     2341 agtgccgtcg gtacgggtcg cacggaaacc gatgcggaag atcgagatcc tcggtctgct
     2401 catcttcatc ctcatggcgg tcgtcatgct cgcggtcggt gcggcactct tctgtgactg
     2461 gctcgccgaa cccggttcgg cccccgtggg aacaccggtc gagctcggac cggtatcggg
     2521 ggagctcggg gttctcggca cgatcccggt catggctctc atgattggtg tcgaggtggt
     2581 ggggggattc gcgctgatcg ccctctacat gctgtccgcc gtcgggagag aggggtaggt
     2641 tttaccccat cccggcgccc gatttttcct ggttccgcat tgcagcatcc ctctggtgcg
     2701 agcggcgatc gccccccggc cggtcggcca taaataacgc ttaattttcg cctggtttat
     2761 ggaaacactg actgatcaag cgtaaaatat atatccggac cggttaacat cagatatatc
     2821 ccacctcggg agggaggtgt gtcatttatg gcaaaggata agaagcccca ggagaaaaag
     2881 ccccaggaga agaagcagga caagccccag gacaagnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnngaag ccccaggaga aaaagcccca ggagaagaag caggacaagc
     3061 cccaggacaa gccccaggac aaggctaaga agaactaacc ttcaacctct cctatcatct
     3121 ttttttccgg gcaaatctcc ggttctctgc gatatgctcc gctcttctgc acccccgtga
     3181 gtgccggccg tgcatcgatc ccggactacc gggttctgct tgatattcgt tcccgcgcct
     3241 tcccgcagca gttacccata cgctctcgcc gacaggatct cccgttcact tcccgagacg
     3301 caccgtccgg cgggggatgc ccgacagggt cctctcgccg gcccggggga aggtcccgtg
     3361 cgtggtcgac ctgacgtcct cgacggtgca gaacgcacca gggttcatct cctcgaccat
     3421 ccgggtgatc gggaaaagat cgcagcgccg caccacggaa tagaagatgg tgcatggtcc
     3481 atgaagcccc tccgcggcaa cgaccgtgac cccgtagccg gtgcccctga tggcgtcgat
     3541 gagggcgtga tcctcttcag aggcaatcac ccggagcacg cagatcccca tggcgagcct
     3601 ctcctcaagg aggatgccgg catagttccc ggcggcgaaa ccggccccat aagagatgag
     3661 gtagatcggg ttggtgaact cgttgacgac gaggccgagc gagacgatcc agaagatgat
     3721 ctcgacgaaa gcgattgcgg ggacgatgag gcggccgccc cgggcaagga gtatgatcct
     3781 gagagtctcg aggctgacgt cgatcacccg ggccccaaag accaggagcg ggatgaggat
     3841 cggggtgagg gcgccggcag agagaaagtc catatgggag agtcgcccgg ccgggtatat
     3901 gagggtgcag tcaagagccg gcgaaggccc gggatctgcg cggattacgg gaccgggcgc
     3961 cggagtctgc cccttcccgg gccgtgggcg cgcacctccg gttacgaccc ctgcccctcc
     4021 gtcccgtggc ggaagaggga ggctatatcc gggttcccgg gcccggcctc gacgaggatg
     4081 cagagatccc cggcctcgat caccgtcccg ccgtccggcc cggcgagcgt ctccgtaccc
     4141 ctcctgaccg cgagcaccgt caccgcatac ctccgccgca ggtcggcctc ggcaagggtc
     4201 ttcccggcga tcggggcgcc ctcatccacc cgaacgatcg agacctcggc gttgtccagg
     4261 tgcagcccta tgtcgtggag caacggcacc tgccctgagg cccgccggag catctcgtag
     4321 ttcccggtcc gcatcgaagc gacgagcctg tcgatatcgc tcttctgcac cgggtacctg
     4381 accaggaccc gcgacagcac tttcgctgcg gtctcgaact cttcgcagac gacatcgttt
     4441 gcccctatgg cgtagagggc gagggaattc tccatgtacc gggtccgggt gatgatcgca
     4501 cagtcagggt tcaatttccg cgcaatcccc gtcacctggc gcgccgtggc cgcatccggt
     4561 atggtgatca ccaccacgcg ggcacgctca accccggcat ggacaagaac gccctcgttt
     4621 atggcgtcgc cgaagaagat cggttcgccc ttcttgcgct cctcccgcac cgtcttcggg
     4681 ttgagctcga tagccatgta ggggacgccg gtgctccgtg ccgcctcagc gacgtgccgc
     4741 cctatcggcc cgaacccgac gatgacgata tgcccctctc cgggtccgtt tgcaggggct
     4801 accttcaggg acgcatctct tgccctgagc cataccggca ggggaagacg gcggacgacg
     4861 gcggtcgcct gtggtgccga cgcgatgacg aacggggcga ccgccatcgt cccgaccgat
     4921 accgcaagga agatctgctc gagatcgggg ctgagaagcc cgtgctcgat accggtcctt
     4981 gagaggatga acgcgaactc gccgatctgg ccgatggcaa gcccggtgag gacggccgtc
     5041 cggagggaga cccctgcagg gaggacggca acggccccga tgatcgcctt gaggacgatg
     5101 acgccgatga tgatcgcgac gatcaggagc gcgtgggccg cgaagaaccc ggtgttgagg
     5161 agcatcccca tcgagacgaa gaagaaactg gtgaagacgt cgcggaaggg gatgacggtg
     5221 gagagggccg cattgctgta ctcggactcg gatatgatca ggccggcgag gaaggccccg
     5281 agggcaaggg agaggccggc ccgtgaggtg agccatgccg tcccgaggca gagggtgatg
     5341 acggtgatca tgaagagctc ccggctccgg aggctcgcga cccggtagag gaagcgggga
     5401 acgacccaga gggcgcttgc cgtgacggcg gcaaggagga cgaggccgag ggcgaagttg
     5461 atgagcgacg ggacgacccc ggccgatccc atgccgccga gcatggggac gaggaggatc
     5521 atcggcacgg tgctcaggtc ctgcaggacc gatataccga gcgcgagccg caccggcggg
     5581 gtatccacct ctccccggtg ctggaagacg gtcagcatca cggtggtgct cgtgtgggca
     5641 agcagcatcc caaagaagat cgcttccggc ggcgcgaggc cgaaggccga tgcaataacc
     5701 gcgacgacgc cgattgtcag gcctatctgg agcaccccgc cggtgagcat ccctttcttc
     5761 atatcaagga ggctcttgag cgagagttcc atccctatgg tgaagaggag gaagatgatg
     5821 ccgagttccg cgaggatctc gacgtcggcc cggttggtga tgagaccgag gccgtaaggc
     5881 ccggcgatga tcccggtgag gaagaacgcc acgatcccgg gcatcttcat ccggtagcag
     5941 agggcgagcg ctgcgatgga gagggcgaat atgatcagga tctgttcgag aaaggccgta
     6001 tccatggtat ggtccctggc tgtcgtcccc ggcgggccgc catggtgctg gcgctctcca
     6061 gcgagtgcac ccgccggtga tatagagaaa gttgttgctc ttcccttctg ataaaacccg
     6121 atgaggtgta acctcgcaca gtatgcacga acccggattt tccccgggat tgtcacggca
     6181 gatccggttg ccggggctcg ccggcgggct gctccctctc gctcgcgtct acgcccgctc
     6241 cccggccgcc tcgcaaaaga cccgatcgta gacgcgagcg ctcttctccc aggtgaagag
     6301 gtcccgcacc ctcctcggcc cgttctctgc catcgcccgg taaaggtccg gatcgtcgag
     6361 cacccgcacg atggcgccgg cgagccccga cacatcccct ggcgggacga tgaggccggt
     6421 cacgccgtca tcgacggcga acggtatccc gccgacatct gtcccgatga ccggcgtgcc
     6481 gcacgcctgc gcctctatca gcaccatccc gaacccctcc gcccggtcgc aactcggcag
     6541 gacaaggagg tcggcgctgt tgaagtacag gggcatctcg tcctcgggga tgaaaccggc
     6601 gaatatgata tcttcgggga ctccctcgtc ggcagcaagc ctcctgtacc ggtcgatgta
     6661 gtttcccctg ccggcgacga ccagtttcgc atccagggat tccctgacga ttcgcatggc
     6721 ccggatcagg tactggacgc ctttatgccg gctctccctg tccaggtggc ccacgtagag
     6781 caagatcttc tcgtccccca ggcgatactt ccggcgcagg aagtccgttc ggtctccccc
     6841 gaaccgttcg aggtcgacgc ccggggcggc ataccgcagc ttttcgaggt atccggcgag
     6901 gtgggaagat tgctgcgcat agtaatccga tgtggcgacg attgcactgg agaggtggag
     6961 cgtccgggaa ccgaggagcg ggtagtagca ccgcccgatg atccgggtcg gaaggtcgtg
     7021 ccccgtcagg tcgttgtggt aggtcagaac gtaaggaact tgcatcccgg ccgctgcccg
     7081 ggcggctgca tccgcgatga agggaaccgg catatgcccg tttacgacgt ccgggcgctc
     7141 cgaccggatg aggtcccgga gcgccctctc ccacccgagg ctgacggggg tgctggagac
     7201 cttgaactgg tagggcagcc ggaccacctc aaggccgccg atctcctcgg tttttgcagt
     7261 gttctcctcc ttatgccagt tggaggagac gatggtcagg tcgtacccga atgcccgctg
     7321 cagtctcgta ccgatctcgt agacatattt ctgcacgcca cccatgtgcg ggtagaaata
     7381 cggggtcacc accatggcct tcacgctctc tcaccctcca gatggggtga gggtgcacaa
     7441 tcactcatat aaatctatct tcaagcccgc ggggctctga ggttcacccg acctctcatc
     7501 acggccgcgt atacgctgtc ccatcccacc cccgatcttc gcggggaggt gccggaagga
     7561 aacccccggg gcgccccgct cccaacggcg atatccccac ggttcacccg ggagaagtcc
     7621 ggagggccgg ggatgccgcc cccggaggcg acgcactcga tgaacatctc tccctccccg
     7681 gcaccatgat cgctgcttcc ccctcccgta ccggagaccc ggtgatgtac cgaacctgtc
     7741 gcctttgaga tcccctcagt ttccggcatt atgctgcata tcgtatcccg atggccaggc
     7801 tatccggtga tgaagcgatg tcgcccttct cacccgtatg tcacccccca tagtcctgat
     7861 gccggattga gaccacgaag ttccagaata ggtttaaata cttaaagagg cgatccaccg
     7921 ctcgctacaa cgtagctcac aggcccgggg gggaagtgtt gagtcgggga gccgtctttg
     7981 taaccgacgc gcagatgcgg agctctcttg cggtcatccg gtcgcttggt aaacagggat
     8041 tggatgtaac tgcgggtgag gagacgcggt gtgcgacagg agttttctcg aggtactgca
     8101 ggcggggcct ggtataccca tcgccgaaga aggcggagga gcggttcatc gggtatctcc
     8161 tcgataccct gaggtccggg gagatcgatg tactcttccc ggtcgccgat gcctgcttga
     8221 agcctatcgt cgaccatgaa gaggagatct cgcggtacac ccggatcgcc ctgccgcccc
     8281 gtgacatctt cacggcgggc tacgacaaag gcacgaccct ccgtatcgcc atggaaaacg
     8341 gcatcccctg cccgcgcacg ttcttcccgg agagcccgga cgatgcggcc ggcatagcag
     8401 agaagatcga ttacccggcc gtcgtcaaac ctcgcgtgag ttcaggaagg cggggggtca
     8461 gggtctgcag ggactcacag gacctggtcc aggcttatgc ggcatccgca tcggagttcg
     8521 gccccgtggt gatccaggag ttcattccct acggaggaga gctcggggtc tataccctcc
     8581 tcaacgcaca atcagaaccc cgtgcggtga ccgtccagcg tcggatccgc tcttatccga
     8641 tatccggggg ggcaagcacc ctccgcgaga ccatcaagga cgaacgctcc cgggaggccg
     8701 tcgggatcgc cctccgtctc ctgaaggcga tgcgatggtc cggggttgca atggtcgagt
     8761 tccgggtcga tgcccgggat gggagcctca aactcatgga ggtgaaccct cgcttctggg
     8821 gatcgttgaa tctgtctatc ctcagcggcg tggactttcc ctacctcctc taccggatgg
     8881 tcatggatgg ggatatcgaa ccggtcttgg attaccagga gggggttttg tgccgctggc
     8941 tgctccccgg agatatcctc tggtacctga gcgccccaaa gacctgggag aacctcaaag
     9001 agtttctccg gttcgatacg cccgacgata tcctgtcgct gcacgacccc ggcccgacgc
     9061 tggggtttgc gctcgccacg gcacgctacg cgctcgaccc ggatatgtgg cggtttgtcc
     9121 tgaggcgata acgtggccgt caacgcactc agcatcgacc ttgagttctg gtggtgcaac
     9181 gagttcctga acggctacct gcccggcgac cgcgaggata tgttcaagga gtcgctgagg
     9241 ccgatcttcg catcgcttga tgcattcaac gtcagagcga cgttctttgt ccttggtgcc
     9301 gtcgcggaga ggtatcctga ggtcatccac gatatccacc ggagaggtca cgagatcgcg
     9361 tgccacgcct actcccataa accgctctac tcgatggacc agagagagtt cgagagggag
     9421 ctcggaaagt ccctcgaact gcttgccgac tacaacccgg tcgggttccg ggcgccgtcg
     9481 ttctccctga gcaacaggac gaagtgggca cttgagtcgc tcgagaagta cggttttcag
     9541 tacgactcga gcatcttccc ggtgagaaca ccgctctacg gcgccccgga tgcgccggtc
     9601 gggatctacc gcccctcccg ggacgatatc accgtccacg accccgacgg cccgatcatc
     9661 gaatttccgc tctcgatcgt ccggttcggt gtcaacatcc ctatatcagg ggggttctat
     9721 cttcgttccc tgccaaagca ggttcttgcc tggggtatcg gcagagtggc cgcaaagagg
     9781 ccggcaaaca tctaccttca cccccacgac atctacccgg gtcctccccc cctgaaggtg
     9841 ccgttcctct cccggctcgt cacctaccac ggcgtccaca catcgcttga gaaactgatc
     9901 atgctcatga tgaagttctc gttcatgcct atcggagcgc tccttgacga aatgtcgctc
     9961 ctttctccgg aggcccctgt cccgatggcg cctgcccgcc ccctccgggg ggtgggagga
    10021 agagacgatc cggccgtccc gacgattccc gcacccacgc tgcagctgca gcccttcgat
    10081 ggcgtcagac agcggtatcc ggcccgtgcc gcaagaccga tccacgaaac aggtgatccc
    10141 gatgagacgc tgtaacactc accacgggca ccggggcggc atccccggca ccgggccgat
    10201 ggggagatct gcgggaaccg gcatgtcccg ggcaggcgcg atcccgggat ttgagatagt
    10261 attatatact ggatcgtgta tccagagtct gctcgcagcc gtacgcgcgt tcagggttaa
    10321 aggagtcgta tgtgatggat tggactgaaa agagagtgct tgtgaccggc gccggcggtt
    10381 tcatcggcag ccacctgacc gagagcctgg tagaccacgg tgcggatgtg cgggcgtttg
    10441 tccgctacaa ctcaagaaac gaccccggtc tcctgcggct gctcccaccg gagagactgg
    10501 accgggtgcg tatcctcatg ggcgatctga gggatgccga cgccgtccgg cgtgccgtcc
    10561 gggacgtcga cgtcgtcttc cacctgggtt cgctcatcgc catcccctac tcctacgccc
    10621 gtccccggga gacggtggag acgaacgtca tcggtgcgct caacgtcttt gaggcggccc
    10681 gggatgccgg cgtcgagaag gtcgtccaca catcgacgag cgaggtctac gggaccgccc
    10741 gctacgtccc catcgacgag aaccacccgc tccaggggca atccccctac tcggcgagca
    10801 agatcggggc cgacaaggtc gccgagagtt tctaccggtc ctttgacgtc cccatcgcca
    10861 cgatacggcc gttcaacacc tacggcccgc ggcagtcggc gcgggcggtg ataccgacga
    10921 tcatcacgca ggcgctggtg caggggaagg tggcgctcgg tgcgacgcac acgacacggg
    10981 actacaccta cgtcgacgac ctggtgcgtg cgtttctcct catcgccgaa tcgccgggaa
    11041 cggtcgggga gacgatcaac gtcgggtcga actacgagat atcgataggg gccgtcgccg
    11101 gtacggttgc agccctcctc ggaaaagaga tcgatatcat caccgagagc gagcgcctgc
    11161 ggccggagaa gagcgaggtc gaacggctct ggtgcgacaa caccagggcg cgggatctcc
    11221 tcggctggtc gcccgccgtg cccctggaag aagggctttt gcgtacgatt gcatggtttc
    11281 gggaacacct gggcctctat cggcccaacc actacgtgac ctgatatgaa agcgattatt
    11341 cttgccggcg gcaagggacg aaggcttgaa ccctacacga ccatcatccc caagccgctt
    11401 atgccgatcg gggataaacc gatccttgag atcatccttt gccagctccg tgagcacggt
    11461 atccgggacg tgacgattgc ggtcgggcac cttgccgaac tgatcatggc gttcttcggc
    11521 gacgggagca ggtttggtct ctcgatcacc tactcgcggg aggagaggcc gctcgggacc
    11581 gcaggctgcc tcgggctcat caaggacgac ctcatcgaac cgttcctcat gatgaacggc
    11641 gacgtcctga caaccctccc gttctcggag ctcatggact ttcaccaaaa aagcggcgcc
    11701 atcgcgacgg ttgccctcaa caagagggat tacttcgtcg attttggtat cgtggatacc
    11761 aacgggaacg gagatatcgt cgggtacacc gagaaaccgg ggtttgaaca ctacgtgagc
    11821 atgggcgtct acgcgttcga cccgagagtt ctcgcgtata tcacgcccca cgagtacatc
    11881 gacttccccg acctgatcaa ggccctgctt gcggcgggcg agacagttaa agcctacaaa
    11941 tacggcggct actggctgga catcgggagg gtcgatgact acaagaaggc gaacaacgag
    12001 atcgccgggc tctatgggac gctgggcgtc gggggcgagt gatatggcgt ggaagatccc
    12061 tctctcagac atcgccatgg acggcgaaga ggtcgatgcg gtcagggatg tcctgcagtc
    12121 gaggtggctc tcgatggggc cggtgacgga ggagttcgag gcggcctttg cccggtacct
    12181 cggtgccgaa catgcgtttg cagtctcgaa cgggacggcg gctctgcaca tcgcccatgc
    12241 ggtgctcggc atcggcagcg gcgacgaggt cataaccccg tcgctcacgt tcgtcgcgac
    12301 ggcaaactcg gtcctctact gcggcgcaag accggtcttt gccgatattg ccggcccgga
    12361 tgacctgaac gtggcatggg aggagattgc ggcaaagatc acgccggaga cgcgggcgat
    12421 caccgtcgtc cactacgggg ggtatccctg cgatatgcaa ccgatcctgg atcttgcccg
    12481 cgatcacgac ctccgggtcg tcgaggatgc ggcgcatgcg gtcggtgcga cctaccgggg
    12541 gcagaagtgc ggcaccatcg gggacgtcgg gtgcttcagc ttctttgcaa acaagaacct
    12601 cgccaccggc gaaggaggca tggtcgtgac ggatgacgac gaccttgccg aacggatccg
    12661 ggtcatgcgc tcgcacggta tgaccacgct gacctgggac cggcaccggg ggcacggcta
    12721 cagttacgat gtcaccgcta tcggctacaa ctatcggacg agcgaggtcg cctccgcgct
    12781 cggcatcgtc cagctccggc gcctcgcgga gaacaatgcg cgacggaaac gtatcgccgg
    12841 ccgctaccgg aagctgctcg agggggttga aggcatagag tgcccgtttg ccggggcggt
    12901 cgacaaagag tcctcccatc acatcttccc ggtcgttctc tcacaggatc tctcgcgggc
    12961 cgggcttcag gaggcgttga aggagaaagg gatccagacg agcatccact acccccccat
    13021 ccacctcttc acctactacc gcgggctgtt cggggatacc cggggctctc tcccgaagac
    13081 ggagtttgcc ggggagcacg agatgacgct gccgctctac cccggcatga ccgatgatga
    13141 ggtggagtac gtctgcaccg ccatcgccgg ggctgcaccg ggcatcctgg cgggaggccg
    13201 gggggcggca tgatcgccga tctgcacatc catacccgtt actctcacga ctcgttcatg
    13261 gatccggcca gggtcgtaag agtagcccgg atgcggggcc tctccgccat agccgtgacg
    13321 gaccacaaca ccatccgcgg cggtctcctt gcccgggagg cgaaccctgg tggggacttt
    13381 gaggtcatcg tcggcgccga gatcaggacg gagtacggcg acgtcctggg tctctttctt
    13441 gaccgggaga ttgctgcgcg ccggtttgac gacgtggtcg aggagatcca cgcccagggc
    13501 ggccttgcgg tcctcgcaca cccctaccgc cggtacccat cgcccgagcg cctcgcggga
    13561 agggtggacc tcatcgaagg gttcaacagc cggtcgaggc ggcaggcgaa cgcgctctcg
    13621 atgcggttcg ggcgagcctc cgggaagggg acggtcggcg ggagcgatgc gcatacctac
    13681 gcggagatcg ggaggggtgt gacgatctat gccggaagtg agatcgaaaa ggcgctccgt
    13741 gccgggcaga ctgccgggga aggccgggag tcgaactact atgtcgtaca cgggttgagc
    13801 tgcggtatcg agaggttaaa acgtctggtt cccggccggg gttgagtgct cccggcggga
    13861 gtactgttcg tggcccctct gaccaatacc ggccggcatt ggtcttgcct gggtttctca
    13921 ctggattata tttatatatt tggtttacta tcaccactcc gtatccacta tctcggggga
    13981 aggaatgtta gcacggtctc gagcatactt aaacgtcgaa cagatcgtcg atgatctgtc
    14041 acgtgaggat cttgaggatg agagtcatat cagaaccttt gaggaccggt tctgcagtta
    14101 catcggggca gaacggtgcc atgcgacaaa ccaggcccgt gcagccctgt tgatagcgtt
    14161 gcgggccctc gacctctcgc cgggcgacga ggtgcttgtc ccggacttca ccttcgaggg
    14221 cgtcgtcgat gccatcctgg agtccggagc gaccccggtc ttcgtcgaac cggcgcttca
    14281 ggacttcaac gtcccggcat cgcattttat cgagaagatg acggaacgga cacgggcggt
    14341 cattgcgacg catctcttcg ggatcccctg cgatatcggg gacctgccgg cgatcgcgaa
    14401 agagaacgat tgcgttgtca tcgaggactg cgcccagtgc ctcggcgcgg agtataacgg
    14461 gaggcccgtc gggacgttcg gggattttac gatcgtcagc ttcaactacg aaaaacacct
    14521 cacgaccggt gaaggcggga tgctggccgt caaca
//
LOCUS       DAOSEE010000047        14454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1654_length_14454_cov_205.065005, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000047 DAOSEE010000000
VERSION     DAOSEE010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 14454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14454
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1654_length_14454_cov_205.065005"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="PLI76_05085"
     CDS             <1..436
                     /locus_tag="PLI76_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOI61439.1"
                     /translation="HGDCGTQSMLFSAFCRSLGIPARAVGGYQMIMAGTPGGHFWAEY
                     YLPGYGWVPNDVTIAEAADWVVIPEEKRSAFKDYYAANLDPARLVIQKNVDTPLDPAL
                     PDDAAIFRVVRQCPAIVSDTADYDIDLFGMGCFGISLETVDQ"
     gene            complement(553..3193)
                     /locus_tag="PLI76_05090"
                     /pseudo
     CDS             complement(553..3193)
                     /locus_tag="PLI76_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868227.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            3448..3756
                     /locus_tag="PLI76_05095"
     CDS             3448..3756
                     /locus_tag="PLI76_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1894 domain-containing protein"
                     /protein_id="HOI61440.1"
                     /translation="MASRCVNNLGGQVLLKDVTPEQVNDYVRRHCKEYYEMPPGFVFR
                     DIRMLLKSPMLVGLQNRKKRVLLPFTKPCPGYGTMLYEITAKEADLDLIRSNLAKLSE
                     "
     gene            3894..4523
                     /locus_tag="PLI76_05100"
     CDS             3894..4523
                     /locus_tag="PLI76_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOI61441.1"
                     /translation="MGIKEWVVPQDKVFFDLFDRMARTVVIAADLLVEFVENFENVKE
                     QCYRMKQLEHQGDDITHQIYEQLNRTFITPLEPEEISRLASALDDILDYIDGTVQQMY
                     GYGITETDASMVELAKLIQLSVIEIEKAVNSIRSIKDPGLIEERCIEVNRLENIADNV
                     LGHAIMDLFKTKDAITIIKLKDIYENLEIATDKCEDVANVLSDIAIRHT"
     gene            4529..5713
                     /locus_tag="PLI76_05105"
     CDS             4529..5713
                     /locus_tag="PLI76_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOI61442.1"
                     /translation="MDPIIILGILLALLFNFANGLNDAANSIATIIATKALSPLQAVL
                     LAGVFNLLGPLLFTTAIAATIGRGLVDPAFLTPPMILMAMVGAVLWVLATSYLGIPVS
                     SSHALIGGLLGAGIAAAGMGVVFWPSLTAIGQVLFYGAVGAVLGAIGTSAYTLRKGEF
                     APWHLFLGGLIGVTLLIPSAIVLGFLKINGILAILIFIVISPTLGLISAFAFGILVAR
                     AFCDYPPRRLNSLFRNLQIFSGSLQAVGHGGNDAQNAMGIITAMLLAGGLISEFAVPL
                     WVILVSSLAISIGTLLGGWRVIDKMANRITRIRPYQGFAASTAAGGVLSLMNVFGVPV
                     STTHAITGAIMGVGATRGYSAVKWGVVREILIAWVLTIPAAAIVAGICFLVARGLLGG
                     IF"
     gene            complement(6046..6360)
                     /locus_tag="PLI76_05110"
     CDS             complement(6046..6360)
                     /locus_tag="PLI76_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor S"
                     /protein_id="HOI61443.1"
                     /translation="MMFCPQCKGLMISSGGQLKCKKCGYIREINDLDRLKKTDKRVTK
                     EIVIVDAEDTIATLPTATIKCPDCECTTAFWWLRQLRAADESEVRFFRCTACGKTWRE
                     YD"
     gene            6488..7318
                     /gene="artA"
                     /locus_tag="PLI76_05115"
     CDS             6488..7318
                     /gene="artA"
                     /locus_tag="PLI76_05115"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase A"
                     /protein_id="HOI61444.1"
                     /translation="MKDYLVLASCICFLVFLVPGRFRKYFAIGGWAGIVGYLFLELPY
                     YFSINNFMYPTIALLSVPFLAITVQHLLRDDPRVMQLSTIAAVAFLIYAPFGFIPALG
                     DWLIAAVADQTSAALAASGHPVNFSAWNTMERNGFRTEIILACTGIQSIAIMLGVAWG
                     VPSTARQKLTGFLLVFPTIYILNIARNVFVITAYSEQWFPYLPAIAGNGEFGYESFFW
                     AHNVMAELGALIFLVALAYALFLILPELGTLADSLYRLYRGEVERVLRPKTPQSGLQP
                     "
     gene            complement(7614..8246)
                     /locus_tag="PLI76_05120"
     CDS             complement(7614..8246)
                     /locus_tag="PLI76_05120"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOI61445.1"
                     /translation="MNITSLRPKFIKYSEPVASFFIRLGITPNQVSMLSVLFGLSCAL
                     AFSQRYFLAGSLLLVVSAVLDLVDGSVARRNHTESRFGAVFDWVADKYVDAAVILGVG
                     FSGIPIVSHLVSVPPIADFGVVGIALAGSLINTFIKPVTYAEIGYTERIAGKIEDPLE
                     GVGFFGRPETILALVLGGVTGYIWAAILLIAVCTNLSAIQRILYLYRQYS"
     gene            8373..9065
                     /locus_tag="PLI76_05125"
     CDS             8373..9065
                     /locus_tag="PLI76_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HOI61446.1"
                     /translation="MADHERPHVLMMSEITVDGKLTLRRGASSKILMKYMAPETEILL
                     HRTRAECDAIMVGANTIRIDNSFLTVRLVEGKSPLRVIPSNRGDIPPDAHILGPDART
                     VIAVSGAAPAEKVAHLREKGVDVVVAGEERVDLPGLMRILKADYNVSRMMIEGGPTLN
                     WSMLNHRLVDEIRLIHLPFIVGGADTPSLVGGMHIETENDMIRLSHKRHYMCGSNLVT
                     EWDVLYERSART"
     gene            complement(9103..10374)
                     /gene="truD"
                     /locus_tag="PLI76_05130"
     CDS             complement(9103..10374)
                     /gene="truD"
                     /locus_tag="PLI76_05130"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0031119 - tRNA pseudouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HOI61447.1"
                     /translation="MMPSPYPLEENLGMRYYASSTPGIGGRLRSSPADFVVEELPLPI
                     SDADGPYIICRLTKTNWELQRAAREIAKQLAISHRRIAWAGNKDKNAVTTQFLSIYGV
                     TPEEAGRVHLKDISLEVVGRSQHPLVLGGLAGNRFDIIIRDCVPEELAQRVRAVTEVV
                     AAGVPNYYGLQRFGVVRPVTHIVGEKILKGDYEGAVAAYIGCAYPGEPGDVQRARSHF
                     AETRDAGAALAGLPIRMTYERSMANHLVANPGDYAGALRVLPPKLLSLLVSAFQSYLF
                     NCALSSRIDAGIPLFEPEVGDRLLFHDGREDIVTARNRQTALVHIRRGRCGIAIFIPG
                     AEPVAPGGRMDEIMQELMQNHGIDAKDFARASRFVETAFAGVARPIALSAGVEADVMD
                     ASVRLRFTLPPGHYATTVCREYMKADPYAMI"
     gene            complement(10371..10733)
                     /gene="pth2"
                     /locus_tag="PLI76_05135"
     CDS             complement(10371..10733)
                     /gene="pth2"
                     /locus_tag="PLI76_05135"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-tRNA hydrolase Pth2"
                     /protein_id="HOI61448.1"
                     /translation="MPDIREFKWKQCLIVRADVRMSCGKKCAQAAHAAIGAYEKADKL
                     VKKAWYDEGQKKVVLKAANERQLFELKTIAERAGIPASIIQDAGMTEIPPGTVTALGL
                     GPARTEDLDRITGDLPLL"
     assembly_gap    10784..10883
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10969..12117
                     /locus_tag="PLI76_05140"
     CDS             10969..12117
                     /locus_tag="PLI76_05140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOI61449.1"
                     /translation="MWDVVVVGAGPAGSAAARACAEEGLTTLCIEEHGTIGYPVQCAG
                     LLSASALAECDVSKRSVTNEVSGARMVSDLGGELLFDARTTKAYVVDRCLLDREMAEN
                     AANAGAEFRLKTSASGIRGTALLTRGIRGREEIPFRLLIAADGPRGSVARMFGLRRAA
                     TYLAGVQADVPCAVDPRYVELHPNASPDFFGWVIPVTATRARVGLCAREGAKDHFERF
                     IARYGETTLNLTSGVIPLGVMPQTYGSRALFVGDAAGFAKPTSGGGVYTGVRSAKHAA
                     AVAAACLARGTLDDGSLRDYERRWREDFGRELDIGMKALRMRQKMTPQEIDRLCRVLN
                     DPDIIETIVEHGDMDRPGTLIRKLALKPRLARAMGILFASGVRQILTG"
     gene            12181..12819
                     /gene="cbiM"
                     /locus_tag="PLI76_05145"
     CDS             12181..12819
                     /gene="cbiM"
                     /locus_tag="PLI76_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt transporter CbiM"
                     /protein_id="HOI61450.1"
                     /translation="MHIPDAFIPIEQALAYWVIALIFIALSLRWARRSMVEEKVPLVA
                     VLAAGIFAIQALEIPIPWGTSGHMIGAALAAIVLGSPFAGVFVLTLVILVQALVFGDG
                     GITVMGANIIDMGVVGGFVGYYGYTAVMGIAKNPYVAAFIAGWASLFISATLVAGQLA
                     IAGTFPLDIGLAFMAGYHAVIGLIEGGITAVALYLIASARPDILERSTEVTA"
     gene            12819..13169
                     /locus_tag="PLI76_05150"
     CDS             12819..13169
                     /locus_tag="PLI76_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDGLE domain-containing protein"
                     /protein_id="HOI61451.1"
                     /translation="MEKAQFIVTGIAVALVIAAAAPFFASGNPDGLESAFFSIHGAKD
                     FQGNDLDEDAAAAAEEKVVAVTGNDFSYGAPLPDYGIAGMDKPGEALAGVVGTLLVLA
                     LAYGVARVAARPDN"
     gene            13198..14031
                     /locus_tag="PLI76_05155"
     CDS             13198..14031
                     /locus_tag="PLI76_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOI61452.1"
                     /translation="MHLVETRNLTHVYRGDIPALGGVDFIAGRKSRIAVIGPNGAGKS
                     TLFKHFNGILKPTSGEVLVRGEPITRENIREVRKFVGIVFQNPDDQIFSPTVEQDVAF
                     GPTNLGLDEATVAHRVGEALSLLGIEDLRERVPHHLSGGEKKRVAIAGILAMEPQVVV
                     LDEPTAGLDPRGVADLVAFVNHLPEEYGMTVIFSTHQVDLVAEMADFVYVIDQGKVAA
                     TGTVEEIFDRPELLARTRLDVPVIPRLIRSLQEEGIAIDMAYTYDGAKKSFFDACTRR
                     P"
     gene            14028..>14454
                     /locus_tag="PLI76_05160"
     CDS             14028..>14454
                     /locus_tag="PLI76_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiQ family ECF transporter T component"
                     /protein_id="HOI61453.1"
                     /translation="MIDDLYSIEESAYRDSVIHRLDARVKLLVALAGIVAVVAMPYST
                     RIYELGAVLFALSLVLWVFSRLSPAIYLRRLALILPFGIFIIGFQVFVKNPYYEVFHP
                     IAVLPFGIAIYAESVEFASILLVKFIVSISFIILLSSTTK"
BASE COUNT         2540 a         4629 c         4443 g         2742 t
ORIGIN      
        1 tcatggcgac tgcggcaccc agagcatgct cttctccgcg ttctgccggt cgctcgggat
       61 ccccgcccgc gccgtcggcg gctaccagat gatcatggcc ggaacacccg gcggccattt
      121 ctgggcggag tactacctgc ccggctacgg ttgggttcca aacgacgtga cgattgccga
      181 ggcagccgac tgggtcgtca tccccgagga gaagcggtcg gcgttcaagg actactacgc
      241 cgcaaacctc gatccggcac gcctcgtcat ccagaagaac gttgacactc cgctggaccc
      301 ggccctgccc gacgacgccg ccatcttcag ggtcgtcaga cagtgcccgg cgatcgtctc
      361 cgatacggca gactacgaca tcgatctctt cggcatggga tgtttcggga tcagccttga
      421 gactgtcgac caataatccc ctctttttat cccggaacgg ttgcctccgg cacccgggga
      481 tctgcatcgc cgggtccgcg gggactcccg atatgacgat ttacacccac gcggagaggg
      541 atgtgagggt tctcagcacc cccgaccgat atcgtcgacg gccgcaacca ggaggtcgat
      601 ctcctcttct gtcgtgtagg ccgcaacgct cgcccgcacc gtccctccct ccaggaggcc
      661 gaggtgctcc atgagcggcc ggcagcagtg gagccccgag cgcacctgta tatccgcatc
      721 ctcgtcgagc acccgcgcga cctcctgtgg gtgggtgccg tcgacggtga acgagacgat
      781 gccgatccgg gcatcaggcc tcctgccggc gtagaccccg accccgtcga gagcggagag
      841 ccccctgatg agccgggcgg tcaggtgttc ttcgtgccgc cgtatcctct ccatccctat
      901 cgccgagagg taatccacgg cgacgccgag ggcgataccg ccgccgatgt tctgtgtgcc
      961 cgcctcatag cgccggtagc cttccgccgt cacgaaccct tcggccgtca cgctctcgac
     1021 catgccgccg ccaaggtccg tgggctcaag tatcgcctcc cgcatccaga ggacgccggt
     1081 gccggtcggg ccgaataact tgtgccccga gaaacagagg aagtcgcaat cgatctgcgc
     1141 gacatccacc ggcatatgcg gcagggactg tgcgccgtcc acgaggagca tggcgccgtg
     1201 ttcgcggcag agccgggcaa tctccgcgac cggcgtcgtc acgccgagga cgttagaggc
     1261 gtgagtgacc gtaacgagcc ggacgccgcc gccggcaagt gcaccctcga gtgcggcaag
     1321 gtcgagcgcg tagtcggcat cgatcccgat catgtcgagg gcgacgccct gtttcgcgag
     1381 cgctctccag ggcaggaggt ttgaatggtg ttcgaggacg gtcgttgcca cccggtcgcc
     1441 cggtttccag gagagcccct gcgcgaccat gttgatcgcc tccgtggcgt tcttcgtaaa
     1501 gaccgtgacc cctgcctcgc cgccgatgaa ccgggccact ttctcgtggg cgtgctcgta
     1561 gcgttgcgtc gccatctgcg ccaggcggtg aaccccccgg ccgacgttag accgatagcg
     1621 gtgctcgaaa tcgacgagcg cctccaccac cggctccggc gagaaacccg tcgatgcgtt
     1681 gtccaggtag acgatgccac cgaggatagg gaagtccttc cggatctctt cgggggtgaa
     1741 ctctgctcct gccggcggcg ccggctcacg ggatcgtacc gtcttcatcg ctgccacatc
     1801 cgctctctcc cctacccgtg actgcccgca atgtaaagtc atcgttgaga gcgatccccc
     1861 tgtccctgca gatctcgcgc atcttcgggg gcagcacccg ccagcgccag agcccccacc
     1921 ggtggtaagc gtccggcatt cccgtctttt ttgcccatcg cacgaggaac gcatcccagc
     1981 gttccatcag gtcggggtgc atcgcccgga ggccttcgta ttcgctctcg agcgccgccg
     2041 ggcagaggta acagcctatc cgctcaagac ccttctcgta gagggggttt atggggattt
     2101 tctgccacca gatgtagagg aagacctcaa gcgcccgcca gttccggatg ggcgatatgt
     2161 tcagctgcag ggggtttgcc gggttctggc tcgtctcctc aaggtcggca cggttccagg
     2221 actcgtacca gcggttcccc tgtatggtga cgcaggggcc gatgtcggcg aggtagctct
     2281 tcagggggtg gagtttcaag agcttacagc accagcggcg gtcctttccc ggcggtcccg
     2341 ccttctcggc cgcctggaag aagtcgccgc tttttcggat gatctcgacc ccctgcgact
     2401 ccacgaactc caccgtctcc ggcagttcga taccggtgtc gatgaagaac gccttctcca
     2461 caccggcctt tctcgcaagg tggaggacga cggcgctgtc cttgccgccg gagaacgaga
     2521 cgttcacgca cggccggtcg ctcatgtgct tcttgacggt gcggatggcg ttgcgctcaa
     2581 ggttcttcag gtggtagcgg ttcttctcga tcaccgtgtc ccatcccggg ttgggcctgg
     2641 gtcggggggt gacgggaacg agttctttca cccggacctg cccgtccctg acgatgccgg
     2701 tgccgaaccg gttcctgtag gagacgatga ccgtcccgtc gggcacgggg gccgcgaggg
     2761 ggaaccgctt cccgccgata cgccccctgt gggcgatcac ggccggttcg gcctcgaggt
     2821 cgaggatacc gcgcgttgcg tgcggcagta tgtgcgggag cgcctcgggg gcgatatcga
     2881 ggctgaactt ccgggcgacc gggtcgaacg agagccagcc gaaccggtcg ccgtgcacga
     2941 tcacgaggtc ggccctgtcg gcgccgccgg ttttgttcag gagcacgacc ttttgcaggg
     3001 gaacgtcgcc gaactgttcg gcaagcaggc tccgtatgag ggccatatcc gctgcaaggg
     3061 ccgggcggac gtcatggggc tgcaggaggg atatctccct gactccggtc ccgcaggcgc
     3121 aggtgcgccc gatgaggggg acgttgcacc ggtcgcacca gtagagaact ttttttactg
     3181 ccggttcgcg catcacgtgg tgaatagttg gccgccgcga gcattaagtg aaccgatgat
     3241 acggatccgg ttctcgtgag gagagggatc ggccctgtgg acggccgcag tcccggtatc
     3301 cgccgcatcc gggcgacggt ctcgcacctc ctccaccccg gcgcaccatg ccgacgatcg
     3361 tcgcgccctc gccgtcaggg tgatccacag cttgatcccc ggacggtagc ataaaattta
     3421 ttcgtattga tgcgaattat ctatgcgatg gcatcgcgat gcgtgaacaa cctcggcgga
     3481 caggtcctcc tcaaggacgt gacgccggaa caggtgaacg actacgtgcg caggcactgc
     3541 aaagagtact acgagatgcc tccgggtttc gtgtttcggg atatccggat gctcttgaag
     3601 tcgcccatgc tcgtcggcct ccagaaccgg aaaaagaggg ttttgctgcc gttcacaaaa
     3661 ccctgccccg gttacggaac gatgctctat gagatcacgg caaaggaggc cgaccttgat
     3721 ctcatcagga gcaacctcgc aaagctatcg gagtgatgcg ggccgccgta cagcgcccgg
     3781 cacgctgcag agaacgtcct gtcctgcttg cccggcagca ggacgggatc gtacggaagt
     3841 tgcctgaccg atcgctatat agcccccgac aggaaaacct tatagcgatc attgtgggca
     3901 ttaaagagtg ggtagtccct caggataagg ttttttttga tctctttgac aggatggcgc
     3961 gaactgtcgt tatcgcggcc gacctgctcg tcgagttcgt cgagaacttt gagaacgtca
     4021 aggagcaatg ctaccggatg aagcagcttg agcaccaggg cgacgatatc acgcaccaga
     4081 tctacgagca gctgaaccgg acgttcatca cgccgctcga accggaggag atatcccgcc
     4141 ttgcgtcggc actggacgat attctcgact acatcgacgg caccgtgcag cagatgtacg
     4201 gttacggcat caccgagacc gacgcctcga tggtcgaact tgcgaagttg atccagctca
     4261 gcgtcatcga gatcgagaaa gccgtcaaca gcatacggtc gatcaaggat cctggcctga
     4321 tcgaggaacg ttgcattgaa gtgaaccgcc tggagaacat cgcggacaac gtcctcggcc
     4381 atgccatcat ggacctcttc aagacgaaag atgcgatcac catcatcaag ctcaaggata
     4441 tctacgagaa cctggagata gcgaccgata aatgtgagga tgtcgcaaat gtcctgagcg
     4501 atatagccat cagacacacc tgaccgctat ggacccgatc atcattctcg gcatcctgct
     4561 cgcgctgctc ttcaacttcg cgaacggtct gaacgatgcc gcgaactcga ttgccaccat
     4621 catagcgacc aaggccctgt cgcccctgca ggcggtgctc cttgccgggg tcttcaacct
     4681 cctcggccct cttctcttca ccacggcgat tgcggcgacc atcgggaggg ggcttgtcga
     4741 ccccgccttc ctcacgccgc cgatgatcct catggccatg gtcggcgcgg tgctctgggt
     4801 cctcgcaacc tcgtacctcg gtatccccgt atccagcagc cacgccctga tcggggggct
     4861 cctcggcgcc ggcatcgcgg ccgccgggat gggggtggtc ttctggccgt cgctcacggc
     4921 gatcgggcag gtcctgttct acggggctgt cggcgcagtc ctcggtgcga tcggcacgag
     4981 cgcgtacacg ctccggaagg gcgagtttgc gccgtggcac ctcttcctcg gggggctcat
     5041 cggtgtgacg ctgctcatcc cgtctgcgat cgttctcggg tttttgaaga tcaacggcat
     5101 ccttgccatc ctgatcttca tcgtcatctc ccccacgctc gggctgatat ccgcgtttgc
     5161 cttcggcatt ctcgtcgccc gcgccttctg cgactaccct ccacggcgcc tgaacagcct
     5221 ctttagaaac ctccagatct tctcgggatc gctccaggcg gtgggccacg gcggcaacga
     5281 tgcccagaac gcgatgggca tcatcaccgc catgttgctt gccggcgggc tgatctctga
     5341 gtttgccgtc ccgctctggg tgatactggt ctcttcgctt gcgatatcca ttggaaccct
     5401 cctcggggga tggcgggtca tcgataagat ggccaaccgg atcacgcgga tccggcccta
     5461 ccaggggttt gcggcgtcga ccgccgccgg aggagttctt tcgctgatga acgtcttcgg
     5521 ggtcccggtc tcgacgaccc acgcgataac cggcgcgatc atgggcgtcg gcgcaacgcg
     5581 cggttattcg gcggtcaagt ggggcgtggt ccgcgagatc ctcatcgcgt gggtcctgac
     5641 cataccggcg gcggcgatcg tggccgggat ctgtttcctt gtcgcccggg gtttgctcgg
     5701 cgggatcttc tgatgagggc ggaatgggag agaggccggc ccggctgacc cattgccagg
     5761 tcgtcgccac ctgtaacgac acgccgtatg ccggcgtccg ggggagggtg atcccggtgc
     5821 cggggggagt gagagaaggc gatctgaccc cgcgttttcg tatgaagata cccttcgcaa
     5881 ttttcatccg gcgtgcctgc ggccccacgg ggcatcgcgt aatttttgca attgccccgg
     5941 aggctcagtt accggcctat acctcctgca ttcgcgagga acgatatccg gcactctcaa
     6001 aaaccgccgg aagaagaaga gggcccggag ggcaacagat tctacttaat cgtactcgcg
     6061 ccacgtcttc ccgcaggcag tgcagcggaa gaacctgacc tcgctctcat ctgccgcccg
     6121 cagctgccgg agccaccaga acgccgtcgt gcactcgcag tcggggcact tgatggtcgc
     6181 ggtcgggagg gttgctatcg tatcctctgc atcgacgatg acgatctctt ttgtcacgcg
     6241 tttgtctgtc ttcttcaacc ggtcgaggtc gttgatctcc cggatatatc cgcatttttt
     6301 gcacttcaac tgaccaccgg acgatatcat caggcctttg cactgtggac agaacatcat
     6361 cattatatgt ggggcaccga ctgaaattaa tccttggtcg gccggacaac cggggccgca
     6421 cggacaccgg cgcaggccag atccggcgca caaatgattt attgccggat acgccgaaag
     6481 gtaaaggatg aaggattacc tggtgctcgc ctcctgcatc tgctttctcg tcttcctcgt
     6541 cccgggacgg ttccggaagt acttcgccat cggcggatgg gcgggcatcg tcggatacct
     6601 cttcctcgag ctcccctact acttctccat caacaacttc atgtacccga cgatcgccct
     6661 cctctcggtc cccttcctcg ccatcaccgt acagcacctg ctccgcgacg atccccgggt
     6721 gatgcagctc tcgacgatag cagccgtggc gttcctgata tacgccccct tcggcttcat
     6781 acccgctctc ggcgactggc tgatagccgc agtcgccgac cagacatccg ccgccctcgc
     6841 ggcatccggg caccccgtga acttcagcgc ctggaacacg atggagcgca acggtttccg
     6901 gaccgagatc atactcgcct gcaccggcat ccagagcatc gcgatcatgc tcggggtcgc
     6961 ctggggcgtg ccgtcgacgg cgcggcagaa actcaccggg tttctcctgg tcttcccgac
     7021 gatctatatc ctgaacatag cgcgcaacgt cttcgtcatc acggcataca gcgagcagtg
     7081 gttcccctac ctccccgcga tcgccggcaa cggcgagttc gggtacgaga gtttcttctg
     7141 ggcgcataac gtcatggccg aactcggggc gctcatcttc ctcgtggccc tcgcctacgc
     7201 tctcttcctg atccttcccg aactcggcac cctcgccgac agcctctacc ggctctaccg
     7261 cggcgaggtg gaacgggtcc tccggccgaa gaccccgcag tccggcctgc aaccctaagg
     7321 gcgccggcgg tatgccgcca cgaccctgcg ctggcgccgg ggatgcggga ggaagatccg
     7381 gctctgagat ttcgctcatg ccggcacccg gcactgatgt gccttgcgcg acgccttacc
     7441 gcggatgcca ggcctgaggc cgttccatcg ctcatgccct ccgcgaaggg ctcaaaggtc
     7501 gggagatggg cttataactc ccttcgcggg gcggggcccg gcgaatcccg aaccaaatgc
     7561 tcgggcaggt atgacgcggc atagcatccg tatgaagagg cggcagcctc ccttcaggag
     7621 tactgccggt agaggtagag gatccgctgg atcgcggaga ggttcgtgca gaccgctatc
     7681 aggaggattg cggcccatat gtatcccgtc acgccgccga gaaccagcgc gaggatagtc
     7741 tccggcctgc cgaagaaacc gaccccctcg agggggtcct cgatctttcc tgcgatcctc
     7801 tcggtgtaac cgatctccgc gtaggtgacc ggtttgatga aggtgttgat caacgatccg
     7861 gcgagcgcga tgccgacgac accaaagtcg gcgatcggcg ggacgcttac gaggtggctg
     7921 acaatgggga tgccggagaa ccccacgccg aggatgaccg ccgcatcgac gtatttgtcc
     7981 gcaacccagt cgaagacggc gccgaacctg ctctccgtgt ggtttctccg ggccacgctc
     8041 ccgtccacca ggtcgaggac cgccgagaca accagcaaca ggctgcccgc gagaaaatac
     8101 cgctggctaa aagcgagtgc gcacgagagg ccgaagagca cggagagcat cgagacctgg
     8161 ttaggcgtaa tgcccaggcg tataaaaaac gaagcgacgg gctctgaata tttgatgaat
     8221 ttcggccgca gcgaggttat attcatcgca taatttatca gcccgaaaac agtttaactc
     8281 tttgcttcgc cgggcaggga gcgctgtatc ctgtcgggaa tcaccatatt cttaaacgcc
     8341 cccgccagat agatcaggga gaaggagatt tcatggcaga tcacgaaagg ccgcacgtac
     8401 tcatgatgtc ggagatcacc gtcgacggaa aactcaccct gcggcgcgga gcatcgagca
     8461 agatcctcat gaagtacatg gcgcccgaga ccgagatcct ccttcaccgg acccgggcgg
     8521 agtgcgacgc catcatggtc ggggcaaaca ccatcaggat cgacaactct ttcctgacgg
     8581 ttcggctggt ggaggggaag agcccgctcc gcgtcatccc gagcaaccgg ggggatatac
     8641 cgccggatgc acacatcctc gggccggatg cccggacggt catcgccgtc agcggggcgg
     8701 ctccggcgga gaaggtcgcc catctccggg agaagggcgt cgatgtcgtt gtggcgggag
     8761 aggaacgggt cgacctgccg ggactgatgc ggatcctgaa agcagattac aacgtctcgc
     8821 ggatgatgat cgagggcggc cccacgctga actggtccat gctaaaccac cgcctcgtgg
     8881 acgagatacg cctgatccac ctgccgttca tcgtcggcgg cgccgatacc ccatccctcg
     8941 tcggcggcat gcacatagag accgagaacg acatgatccg gctctcccac aaacgccact
     9001 acatgtgcgg gagcaacctc gtcaccgagt gggacgtcct ctacgagagg agcgcgcgga
     9061 cctgaagggg cccgcgccat acgagagatc ttttttgcgc cgtcagatca tcgcgtaggg
     9121 gtccgccttc atgtactccc ggcagaccgt cgtcgcgtag tggccgggag gaagcgtaaa
     9181 ccggagccgg acgcttgcgt ccatgacatc cgcttcaacg ccggcagaga gggcgatggg
     9241 ccttgcgacc ccggcaaacg ccgtctccac gaaccgggag gcacgcgcga agtcttttgc
     9301 gtcgatgccg tggttctgca tcagctcctg catgatctcg tccatccggc cgcccggcgc
     9361 caccggttcg gccccgggga tgaatatggc aatcccgcac cggccgcgcc ggatatggac
     9421 gagagcggtc tgccggttcc gggccgtgac gatatcctcc cggccgtcat ggaagagcag
     9481 ccggtcgccg acctcgggtt caaagagcgg gatgccggca tcgatgcggg acgagagcgc
     9541 gcagttgaag aggtacgact ggaacgcgct cacgagaagc gagaggagct ttggagggag
     9601 cacccggagc gcgccggcgt agtcgccggg gttcgcgacg aggtggtttg ccatcgaccg
     9661 ctcatacgtc atccggatgg gaagaccggc gagcgccgcc cccgcatccc gggtctcggc
     9721 aaagtgcgat cgcgcccgct gcacatctcc gggctctccc gggtatgcac aacctatgta
     9781 cgcggccacg gcaccctcgt aatccccttt gagtatcttc tcgccgacga tatgggtgac
     9841 cggccggacg acgccgaacc gctgcagccc gtagtagttc ggcaccccgg cagccaccac
     9901 ctccgtgacg gcccgcaccc gttgtgcaag ttcctccggc acgcagtcgc ggataatgat
     9961 gtcgaaccgg ttcccggcaa gccccccgag gacgagcggg tgctgcgacc gcccgacgac
    10021 ctcaagcgat atgtccttga ggtgcacccg tccggcttct tcgggcgtaa cgccgtagat
    10081 cgatagaaac tgggtggtca ccgcgttctt gtccttgttc cccgcccagg ctatccgccg
    10141 gtggctgatg gcaagctgtt ttgcgatctc tcttgccgcc cgctggagtt cccagttcgt
    10201 cttggtgagc cggcagatga tgtagggtcc gtcggcatcg ctgatcggga gcgggagttc
    10261 ctcgacgacg aagtccgccg ggcttgagcg gagccggccg ccgatgccgg gggttgagga
    10321 tgcgtagtaa cgcataccga ggttctcttc cagggggtag ggcgagggca tcatagcagg
    10381 ggaaggtcgc cggttatccg gtcgaggtct tcggtgcgtg caggccctag accgagcgcc
    10441 gtcacggtcc ccggcggtat ctcggtcata ccggcgtcct ggatgatgga tgcagggatg
    10501 cccgcccgct ctgctatcgt cttgagctcg aagagctgcc gctcgtttgc agccttcagc
    10561 acgaccttct tctgcccctc gtcataccag gccttcttca cgagtttatc ggccttctcg
    10621 taggccccta tggccgcgtg ggcggcctgc gcacacttct tgccgcagct catcctgacg
    10681 tcggcacgca cgatcaggca ctgcttccac ttgaactccc gtatatcagg catctcctac
    10741 tacctggggc atgatccgtc aaatacgcat ctatgggggc ggcnnnnnnn nnnnnnnnnn
    10801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nnnnnnnnnn nnnnnnnnnn nnngccggcc gtgcccattt atacccgccg gcccctatta
    10921 ttcgaaaggg atcgccgggg tcccgtatac gcgaggcatg gtagtctgat ctgggatgtt
    10981 gttgttgtgg gcgcagggcc tgccgggagc gctgccgcgc gggcgtgtgc cgaggaaggg
    11041 cttacgacgc tctgtatcga ggagcacggc acgatcggct acccggtcca gtgcgcgggg
    11101 ctcctctccg catccgcgct tgccgagtgc gatgtctcaa aacgttcggt cacgaacgag
    11161 gtgagcgggg cacggatggt ctcggacctc ggcggggagc tcctctttga tgcgcggacc
    11221 acgaaggcct acgtcgtgga tcggtgcctc ctcgaccggg agatggcgga gaatgccgcc
    11281 aatgccgggg cggagttccg cttaaagacg agcgcctcgg gcatcagggg caccgctctc
    11341 ctgacccggg gaatccgcgg gcgcgaagag atccctttcc gcctccttat cgccgccgac
    11401 ggaccacggg gttccgtcgc ccggatgttc ggcctccggc gggcggcgac ctatctcgcc
    11461 ggcgtgcagg cggacgtgcc gtgtgcggtg gacccgcgct acgtcgaact gcacccgaac
    11521 gcctcgcccg acttcttcgg gtgggtgatc ccggtcacgg cgacccgggc gcgcgtcggg
    11581 ctctgcgcga gagagggcgc aaaggaccac ttcgagcggt tcatcgcccg ctacggggag
    11641 accacgctca acctcacgag cggcgtcata ccgctcgggg ttatgccgca gacttacggc
    11701 agccgtgcgc tcttcgtcgg cgatgcggcg gggtttgcca aacccacgtc gggcggcggc
    11761 gtctacaccg gcgtccggtc ggcaaaacac gcggccgcag tcgcggcggc ctgccttgcg
    11821 cgcggcacgc tcgatgacgg gagcctccgg gactacgagc ggcgctggag ggaggatttc
    11881 gggcgagaac tcgatatcgg catgaaggcg ctccgtatgc ggcagaagat gacgccgcaa
    11941 gagatcgacc gcctctgccg ggtactaaac gaccccgata tcatcgagac gatcgtggag
    12001 cacggcgata tggaccggcc gggcaccctg atacgcaaac ttgccctgaa accccggctg
    12061 gccagggcga tgggcatact cttcgcatcg ggagtccgcc agattcttac gggttgagag
    12121 aactttctcg cctgggaata cctatatacg aatacgtatt ccaacacgcc ggggtgtgcc
    12181 atgcatatac ctgatgcatt cataccgata gagcaggccc tggcctactg ggtcatcgcc
    12241 ctcatcttca tcgctctctc cctccggtgg gcgcggcggt cgatggtgga ggagaaggtg
    12301 ccgctggtcg ccgtgcttgc ggcagggatc tttgccatcc aggcgctgga gatccccatc
    12361 ccctggggga cgagcggcca tatgatcggg gcggcactcg cggcgatcgt cctcggttcg
    12421 ccgttcgcgg gggtctttgt cctgacgctg gtcatcctcg tgcaggccct cgtattcggc
    12481 gacggcggga tcaccgtcat gggcgcgaac atcatcgaca tgggcgtcgt cggcgggttc
    12541 gtcggctact acggctacac agccgtcatg ggtatcgcaa agaacccata tgtcgccgcg
    12601 ttcatcgccg ggtgggcgtc gctcttcata tccgcgaccc tcgttgcggg ccagctcgcg
    12661 atcgccggca cgttcccgct ggatatcggc ctcgccttca tggccgggta tcatgccgtc
    12721 atcggcctca tcgagggtgg gatcactgca gttgccctct acctgatagc atcggcgcgg
    12781 cccgacatac ttgagcgttc aacggaggtg accgcgtaat ggagaaggcg cagttcatcg
    12841 tgaccggtat agcggtcgcc ctcgtcattg ctgctgccgc accgttcttt gcgtccggga
    12901 accccgacgg cctcgagagc gcgtttttca gcatccacgg cgcaaaagac ttccagggca
    12961 acgacctcga tgaggatgcc gctgccgccg cagaggagaa ggtggtcgcc gtgaccggca
    13021 acgacttctc ctacggggct cccctgcccg actacggcat cgccggcatg gataagcccg
    13081 gcgaagcgct tgccggcgtc gtcggcacgc tcctcgtact ggccctggcc tacggcgttg
    13141 cccgggtggc tgcccgaccc gacaactaaa tacccggacc cctcactttg cgccatcatg
    13201 cacctggtcg agacccggaa cctcacccac gtctaccgcg gcgatattcc tgccctcggc
    13261 ggcgtggact tcatcgcagg gaggaagtcc cgcatcgccg tcatcgggcc gaacggggcc
    13321 gggaagagca cgctcttcaa gcacttcaac ggcatactca aaccgacgtc cggcgaggtc
    13381 ctggtccggg gcgaaccgat aacgcgagag aacatccgcg aggtcaggaa gttcgtaggg
    13441 atcgtctttc agaaccccga cgaccagatc ttctccccca ccgtggagca ggacgtagcg
    13501 ttcggcccga ccaacctcgg gcttgacgag gcgacggtcg ctcaccgtgt cggggaggcg
    13561 ctctccctcc tcgggatcga ggacctgcgc gagcgcgtgc cgcaccacct ctcgggcggc
    13621 gagaagaaac gggtcgccat cgcgggcatc cttgcgatgg aaccgcaggt ggttgtgctc
    13681 gacgaaccga ccgccgggct cgatccccgg ggcgttgccg accttgtagc gttcgtcaac
    13741 cacctgcccg aagagtacgg catgacggtg atcttctcga ctcaccaggt cgacctcgtc
    13801 gcggagatgg cggacttcgt ctacgtgata gatcagggga aggttgcggc caccgggacg
    13861 gtcgaggaga tcttcgaccg gccggagctg ctcgcgagaa cccgcctcga tgtgccggtc
    13921 ataccgagac tgatccggtc gctgcaggag gaaggtatcg ccatcgatat ggcctacacc
    13981 tatgacggcg cgaagaagtc gtttttcgat gcctgcacga ggagaccatg atcgatgacc
    14041 tctactccat cgaggagtcg gcctaccgcg acagcgtcat ccaccggctg gatgcacggg
    14101 tcaaactcct tgtcgccctc gccgggatcg tcgcggtcgt ggcgatgccg tactcgacga
    14161 ggatctacga gctcggtgcc gttctctttg ccctctctct cgtcctctgg gtattctcac
    14221 gcctctcgcc ggccatctac ctccggcggc tcgccctcat cctgccgttc gggatcttta
    14281 tcatcgggtt tcaggtcttc gtgaagaacc cctattacga ggttttccac ccgatcgccg
    14341 tcctgccgtt cggtatcgcg atatacgcgg agtcggtgga gttcgcctcg atcctcctcg
    14401 tgaagttcat cgtcagcata tcgttcatca tcctgctctc gtcgacgacg aaga
//
LOCUS       DAOSEE010000048        14374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1665_length_14374_cov_268.730568, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000048 DAOSEE010000000
VERSION     DAOSEE010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 14374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14374
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1665_length_14374_cov_268.730568"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PLI76_05165"
     CDS             <1..239
                     /locus_tag="PLI76_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division control protein Cdc6"
                     /protein_id="HOI61454.1"
                     /translation="LIDLDVLTHRRITDLISELNMLGVINTRVVSRGRYGRTKEMWFD
                     ATTSKIEEVITRDPRLVDQRLKELDINRLRAMFR"
     gene            244..735
                     /locus_tag="PLI76_05170"
     CDS             244..735
                     /locus_tag="PLI76_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOI61455.1"
                     /translation="MDDKDRILLQLLEENCRTPTNELAVLAEVSEQDIRDRIDRLEAA
                     GVIRRYGAVVDWERAGNGNVAAVIELKVSPERDFGYDRIAERIARFPAVRSLRLMTGA
                     YDLQLLVVGSSMHEIARFVAEQIAPMDRIRETATHIIMKTYKENGTTYAGREEVERLP
                     YSF"
     gene            747..1907
                     /locus_tag="PLI76_05175"
     CDS             747..1907
                     /locus_tag="PLI76_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOI61456.1"
                     /translation="MRDFVSARVRAIPPSGIRKFFDIAQTMEDVISLGVGEPDFVTPW
                     CVCEASIYSIEQGSTAYTSNKGTPQLRGAISRYLDTRFGTCYDPDEEVIVTCGVSEAA
                     DIAIRAVTDPGDEVLVAEPSYVSYTPCVSLAGGVPVTVPCRAEDEFRLTADALMERIT
                     PRTKVLVANFPNNPTGGVMPEADWRAIADILVDHDLLLISDEVYAELTYDGDHFSPAR
                     IPELRDRTITLNGFSKAFAMTGWRIGYLCAPPALAAAALKIHQYVALCAPVMGQIAAY
                     EALRNGEAEKDAMVQEYRLRRNLFVDGLNKLGLACHLPKGAFYAFPSIESTGMTDTEF
                     AESLLYEQHVAVVPGSVLGPSGAGHVRCAYAVSRDNLKKALSRMGAFLESAK"
     gene            2575..3585
                     /locus_tag="PLI76_05180"
     CDS             2575..3585
                     /locus_tag="PLI76_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HOI61457.1"
                     /translation="MSCTIIVGGFFGDEGKGKIVAHIAHQDRPSIISRGGVGPNAGHT
                     VCVGEKEYGVRMVPSGFVYPEAALMIGSGVLVDPRVFLREVDLLGVRGRIFVDGRCGV
                     IEEEHIARDRGSEHLNKKIGSTGTGCGPANSDRVLRTSRQAKDVPELAEYITDVAEDL
                     NCAMDRSEVILLEGTQGFGISLYFGTYPFVTSKDTSASQIAADNGVGPTRIDDVIVVF
                     KAYPTRVGEGPFATEMTMERSHELGIEEFGTVTRRQRRIGTWDGKMARYSAMINGCTQ
                     AAITGIDRIDPACFGVKDYDRLTRKAKDFIAQAEEDIGKPVTLISTGPEMSQIIDLRS
                     EL"
     gene            3582..3767
                     /locus_tag="PLI76_05185"
     CDS             3582..3767
                     /locus_tag="PLI76_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methytransferase partner Trm112"
                     /protein_id="HOI61458.1"
                     /translation="MKRELMDILCCPVCKGDLLLSATEESGGEVLEGTLRCAACRVDY
                     PISEGIPNLLPQTACED"
     gene            3776..4294
                     /locus_tag="PLI76_05190"
     CDS             3776..4294
                     /locus_tag="PLI76_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61459.1"
                     /translation="MSEIHLNRRGINAIEVPGEVEAVPGSDLSLRIINHGSPLHISLA
                     TTNSAPFTDFIHENLYVGGTDAEFHISIREDAPPGVFSVEVIAGYGARRAAFKVFVRE
                     RKAPEPGPVAAPRAEPARTVSLPPLPVLAPVVAALVLYGLWLASGIDLLNAAAFAVLL
                     VGVILAWLRQRS"
     gene            4273..5193
                     /gene="cbiB"
                     /locus_tag="PLI76_05195"
     CDS             4273..5193
                     /gene="cbiB"
                     /locus_tag="PLI76_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HOI61460.1"
                     /translation="MAAAAVVVAASLLVDRLVGDPHSRYHPVALLGRFIGWWGVPSRY
                     PARVQRAAGAAGTILTVALFAAPFALVQFAAPWYLSIILAPFLLKACFAWRSLEEHTV
                     AVVQALEDGGIDAGRARAGMLVSRDTAHLEPEHILSAAYESMTENLVDSIISPLFYFG
                     FFGLAGAAAFRAINTMDAMLGYRDERLRIGWFPARADDIANFIPARITGLVLLVYFAA
                     KGRFSPAYAVLCRDRKKRPGFNGGIPMAVIAGGVGVRLEKPGVYAIGDAERTLTEAGA
                     GIVSAVRAATIIFALAEIGALFLLVSMRYT"
     gene            5199..6098
                     /gene="pdxS"
                     /locus_tag="PLI76_05200"
     CDS             5199..6098
                     /gene="pdxS"
                     /locus_tag="PLI76_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HOI61461.1"
                     /translation="MKLEELRFGTELIKRGFASMQRGGVIMDVVNAEQAELAEGVGAV
                     AVMALERVPADIRAAGGVARMADPQKIVEIIDAVSIPVMGKARIGHFVEAQVLEALGV
                     DMVDESEVLTPADEQFHIDKTRFEVPFVCGARNLGEALRRIDEGAAMIRTKGEAGTGN
                     VVEAVRHMRAIMGGIRSLKGLSSQELIDRAREIEAPAELVIECANLGRLPVVNFSAGG
                     IATPADAALMMQLGADGVFVGSGIFKSSNPEATARAIVEAVNHYDDAKVIAEVSRGLG
                     DAMKGIDIRVLREDEILQTRGRT"
     gene            6310..6684
                     /locus_tag="PLI76_05205"
     CDS             6310..6684
                     /locus_tag="PLI76_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2703 domain-containing protein"
                     /protein_id="HOI61462.1"
                     /translation="MKKELIIEWRHAGSAVNKSPGGVEETGMTLAAVLAEIRMLLEME
                     GVTVRMIETDAEPEGLLFNGIPVERLLEGIEVTALSCSCASCDSCEGDAECRALRYNG
                     EAYEAIPPDLIGRAAVKALEVE"
     gene            7025..8113
                     /locus_tag="PLI76_05210"
     CDS             7025..8113
                     /locus_tag="PLI76_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOI61463.1"
                     /translation="MDVVVITKRCHLLLIMLGVCAVLLCASAACTTARESSPSAEDRT
                     ITDMVDRTVVIPSEIESVLCTSPPSTNLVYMVAPDRLKGWNFAPEKGYTAEKYMALPV
                     IGGWFGKETGNYETFISMHPDIVIEGFTTDGGAASASVAERQQKMEPIPVVAVEDSTN
                     STGYSAPIRFVGDLLGEEEQAEAMIAFYEGVLATVAERAASIPDEERVGVYYAEGPRG
                     LTTDPSGSSHSELIDLCGGRNIADCAITPGMGMTEVSMEQVIAWDPDVILVENPGFYA
                     AVGTDPAWQEITAVKTGRVYLTPRTAFCWFDRPPGINRIIGIPWTAKTLYPDLFRDMD
                     LESLVREYHEIFLHVSLSDDQIHQILNL"
     gene            8124..8765
                     /locus_tag="PLI76_05215"
     CDS             8124..8765
                     /locus_tag="PLI76_05215"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOI61464.1"
                     /translation="MGYTPGRFDRDGSKNMNRIAKTVFAPVYPVIAEQIVERCKISRG
                     RCLDIGSGPGSLGIALARASDLTVTLFDSSSEMLALAEGNIREAGLSGRLSLLSGDVH
                     DIPLPAGSVDIAVSRGSIFFWEDLARAFSEIHRVLAPGGKTYIGGGFGTAELRDAVLA
                     AMTKENPDWKNFRDKNLGPENRERVVGVIEGLGLPHRIVNDDSGFWVMMEKGR"
     gene            8833..10506
                     /locus_tag="PLI76_05220"
     CDS             8833..10506
                     /locus_tag="PLI76_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOI61465.1"
                     /translation="MYTNEGGTIVERYPDRYIAVMPIAIETMPMCHYYPRETFLQASG
                     IGCTFSCGGCISETVALHAEAVSGALKHIPPDALIRRAREEGCRGIAFCLNDPTAMHR
                     TFLAVARAARDAGLLVGCATNGYYTEEALHDLAPAIDFVNIGLKGASDARYRACGAVS
                     AEPAFRAVKTLHEAGVHVEVSCIYARGYDDEIRAVAARIADISPDIPLLVMRFIPFGD
                     ESPEREPTIAESERICDDLRRTLRYVYLFNSPGTGYLDTVCPSCGERVVGREFFGPMG
                     ARTIAVPPDARCLCGFSLPVLGSIGKTPYAEPGMMGGYRFTRALEMVHAVLVCLGVES
                     DADLARIWTGVIKDDYIRDLHGKIQTIDGYTGLVRDLGERSGRPEAAEALAAYIGDRV
                     AAVSSAVEGYPRPRVYYSMGTPLFALNADRFEMDLVEAAGGDPVNRRIVRAGKPGVNI
                     TPEEFAAFNPEYIFISGFLSAPASDYLTACRRMGLSADAIEHGRVYTLPPGWDFGNPR
                     WVLGLSAIAGTLHPACAAFDLAAEEDRFYRTFYGAEASIRTGNRSFYRP"
     gene            complement(10585..11376)
                     /locus_tag="PLI76_05225"
     CDS             complement(10585..11376)
                     /locus_tag="PLI76_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI61466.1"
                     /translation="MILNVEHASFSYDGITKQFEDISFSLRRGEVLSLLGKNGTGKST
                     LIKCILNILSLQSGKVEINGRRIAGMRPDEVAREVGYVPQVHRAAFPFTALDFVLMGR
                     APHISTFSVPKEEDYRKVEETFARIGITHLREKPLPEMSGGESQMVMIARALAQEPSL
                     LVLDEPTSHLDIGNQMKMIATINRLAAEGIGILMSTHFPDHGFMTSHTVAILENGRLI
                     AQGPAEDVITRENLYKAYGVDVCIRYIEEAGRMACIPINSCGVPG"
     gene            complement(11373..12557)
                     /locus_tag="PLI76_05230"
     CDS             complement(11373..12557)
                     /locus_tag="PLI76_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOI61467.1"
                     /translation="MTRGEEPTVEVGQRPEGTIAIELPWVASRGGMPVYEAVLRIFPV
                     EKAIYILPIVLFVFSLFFGRYMMDPVETLRILIVGSATIFLDTASSLLSTFAGHQVLL
                     PSFEQAWGASEQTVIFQIRLPRVIAAMLVGGGLALAGASFQGLFRNPLVSPDILGVAS
                     GAGFGAALGILISGDPRVIQVSAFIFGIVAVAVTYGISRVYRNSSTLILVLGGIVVGS
                     VFSALLSLTKYVADPYDTLPAIVFWLMGSLSAVSNADLLAVAPPILLGGLCLFLIRWR
                     INLLSVGEEEAQALGVDTKKMTAAIIVASTVVTASAVCISGIIGWVGLVVPHIGRMLV
                     GPDFRRLIPVSAVLGASFLLVVDDIARTLTAAEIPLGVLTALIGAPFFAYLLTRKRVG
                     WS"
     gene            complement(12951..13736)
                     /locus_tag="PLI76_05235"
     CDS             complement(12951..13736)
                     /locus_tag="PLI76_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HOI61468.1"
                     /translation="MYPGAKITADPRPGYTRITPFSLPPRQEPAGTNGRAPVFIITGE
                     QGQCKTTFLHLVLGLIVGANVRIRGVLAPGHVRDGRRSGFTLVDLATGEHEELSSVDP
                     DPRCEVHGRFYFRPEGLAFGRRALAPPNPRETDLMAIDEVGRFELQGAVWADQIDRLV
                     SLSHPPMVWTVRRRLLDTVIERWKITNPVVVDVRSASVMATAGDVMDAVWEWREAQTA
                     SAVPVSPFSPGRIAEGPPLLADAAAGASPGAPSIFRRRYGPEG"
     gene            13935..>14374
                     /locus_tag="PLI76_05240"
     CDS             13935..>14374
                     /locus_tag="PLI76_05240"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOI61469.1"
                     /translation="MAEKTVVLHTTMGKIAIRLYGDMPVTAGNFAKLAGSGFYDGTIL
                     HRVIANFMIQGGDPTGTGMGGPGYTIADEFVKGHSNRRGTIAMANTGRPNTGGSQFFI
                     NLVDNTYLDWDDPRTPSAHPVFGEVVEGMDIVDKIGKVRTDSAD"
BASE COUNT         2663 a         4451 c         4633 g         2627 t
ORIGIN      
        1 ggctcatcga cctcgatgtc ctgacgcacc gccggatcac ggacctcatc tccgagctca
       61 acatgctcgg tgtcatcaac acccgggtcg tctcgcgggg gcgctacggc cggacgaaag
      121 agatgtggtt cgatgcgacg accagcaaga tcgaagaggt catcacgcgc gaccctcgac
      181 tcgtcgacca gaggctcaag gaactcgaca tcaaccgatt aagagcaatg ttcaggtgaa
      241 accatggatg acaaggatcg tatcctcctc cagctcctgg aggagaactg ccgcaccccg
      301 acaaacgaac tcgcggtgct ggccgaagtg agtgagcagg acataagaga ccggatcgac
      361 cggctggaag cggccggcgt catccgccgt tacggtgcgg tcgtcgactg ggaacgggcc
      421 ggaaacggca acgtcgccgc ggtgatcgag ctcaaagtct cgcccgagcg ggacttcggc
      481 tacgaccgga tagcggagcg gatcgcccgg ttccccgctg tccggtcgct ccggttgatg
      541 accggggcat acgatctcca gctcctggtg gtcgggtcga gcatgcacga gatcgcgcgg
      601 ttcgtcgccg agcagatcgc cccgatggac cggatccgcg agacggcgac gcacatcatc
      661 atgaagacct ataaggagaa tgggaccacc tacgccgggc gcgaagaagt cgagcgcctt
      721 ccctactcgt tctgaggtga ggtgcgttga gagatttcgt ctcggcacgg gtgcgtgcca
      781 taccgccttc gggcatccgg aagttcttcg atatcgccca gacgatggag gacgtcatat
      841 ccctgggcgt cggcgaaccg gacttcgtga ccccctggtg cgtctgtgag gcgtccatct
      901 actccatcga gcaggggtcg accgcctata catcgaacaa ggggacgccc cagctccgcg
      961 gggctatcag ccgctacctc gatacccgct ttgggacatg ctacgatccc gacgaggagg
     1021 tcatcgtgac ctgcggcgtc tcggaggcgg ccgatatcgc catccgcgcc gtcaccgatc
     1081 cgggcgacga ggtgctggtg gcagaaccga gctacgtctc ctacacccct tgtgtctccc
     1141 tcgccggcgg tgtcccggtg accgtgccct gccgggcgga ggacgagttc cggctgacgg
     1201 ccgatgcgct catggagcgt atcactcccc ggacgaaggt tctggtagca aacttcccga
     1261 acaaccccac cggcggggtg atgccggagg cagattggcg cgcaatcgcc gatatcctgg
     1321 tcgaccacga cctcctcctc atcagcgacg aggtctatgc cgagctcacc tacgacggcg
     1381 atcacttctc gccggcgagg atccccgagc tccgtgaccg gacgatcacc ttgaacgggt
     1441 tctcaaaggc ctttgcaatg accgggtggc ggatcggcta cctctgcgca ccgccggcgc
     1501 tcgccgcagc ggccctgaag atccaccagt atgtcgcgct ctgcgccccg gtcatggggc
     1561 agattgccgc ctacgaggcg ctccggaatg gtgaagccga gaaggacgcc atggttcagg
     1621 agtaccggct tcggcggaac ctcttcgtcg acgggttaaa caagctcggt cttgcctgcc
     1681 acctcccgaa gggcgccttc tacgccttcc cctcgataga gagcaccggc atgacggata
     1741 ccgagtttgc cgagtctctt ctctacgagc agcacgtggc ggtcgtcccg ggaagcgttc
     1801 tcggaccgtc cggcgccggg cacgtgcggt gcgcctacgc ggtctcgcgg gataatctca
     1861 agaaggctct ctcccggatg ggggcgttcc ttgagtcggc aaaatagaaa cgtcaccata
     1921 cgcgcttttg gtatattcaa aaccttttta tatggtatgc ctgtcagggc aactgcctgt
     1981 ccggtgtccg acaggggaac ggcgtgcgga gaaccccttt atttccactg gagatgtgga
     2041 tttattcagg gatcataggt gccgcaggac cccgtcgccc tcatacgggg gtacgcatcc
     2101 cgtcccacaa accccgggca gattacccgg gttcgcatcc gcgctccgtc tgaaagatcc
     2161 gattacagat caacatgact tttccggaaa aatctcgatg tatcggctcc ggatctcatg
     2221 gagatagagt ttccagtgga aatgaagggg tccagacccc tttgtcgacc gggtccgggg
     2281 cgatctgccg ccgcgaaggt tgatatacaa tggcttcctg tcgccggaac cccgggatag
     2341 gcgcatatcg cggcgcagcg ccagaatggc ctgtccagga tctgcgctcc tcccccgggc
     2401 gaccgccagc ctcgcgcggg atgcgatccc ctccggcgct catccgcccg gaacgtgggg
     2461 tggatggtcc ctgcaccccg gaaacccggc tactttcacg caggacgatc gcaccctcag
     2521 tgttgcattg taacgtttat cagggtgtat cgctccatcc tacatgatag aacaatgagt
     2581 tgtactatta tcgtcggcgg attctttggt gacgagggaa agggaaagat cgtggcccac
     2641 atcgctcacc aggacagacc atccatcatc tcccggggcg gcgtcggccc gaatgccggg
     2701 cataccgtct gtgtcgggga gaaggagtat ggtgtcagga tggtcccctc ggggttcgtg
     2761 tacccggaag cggctctcat gatagggagc ggtgtgctcg tcgatccccg ggtcttcctg
     2821 agggaggtcg atctgctcgg cgtcaggggc aggatctttg tcgacggccg gtgcggtgtc
     2881 atcgaagagg agcatatcgc tcgcgacagg gggagcgagc acctcaataa gaagatcggg
     2941 agcaccggga ccggatgcgg cccggcaaac tccgaccgtg tcctccggac gtcgcggcag
     3001 gcaaaagatg tccccgaact tgcggagtac atcaccgatg tggcggaaga cctcaactgc
     3061 gccatggacc ggagcgaggt gatcctcctt gagggaaccc agggtttcgg catatcgctc
     3121 tacttcggga cttacccgtt cgtgaccagc aaagatacgt ccgcctcgca gatcgccgcc
     3181 gacaacggcg tcggtccgac ccggatcgac gacgtcatcg tcgtcttcaa agcctacccg
     3241 acccgggtcg gcgaaggccc ctttgcgacc gagatgacga tggagcggtc gcacgagctc
     3301 ggtatcgagg agttcggcac cgtcacccgc cgtcagcgcc gtatcggcac atgggacggg
     3361 aagatggccc gctactcggc catgatcaac gggtgcaccc aggcggcgat caccggcatc
     3421 gaccggatcg acccggcctg cttcggcgtg aaggactatg atcgactgac ccggaaggca
     3481 aaggacttca ttgcacaggc cgaagaggat atcggcaaac ccgtcacatt gatatcgacg
     3541 ggccctgaga tgtcccagat catcgatctc cggagtgaac tatgaagcgg gagcttatgg
     3601 atatcctctg ctgcccggtc tgcaagggcg atcttctgct ctctgcgacc gaggagagcg
     3661 gcggggaggt gctggagggc accctccggt gcgcggcatg ccgcgtcgat taccccatca
     3721 gcgaaggcat accaaacctc ctcccgcaga cggcctgcga ggactgatcc cgccgatgtc
     3781 ggagattcat ctcaaccgcc gcgggatcaa cgccatcgag gtcccgggcg aggtggaggc
     3841 ggtgccggga agcgatctct ccctccggat catcaaccac ggctctccgc tccacatatc
     3901 tctcgccacg acgaactctg cgccgtttac cgacttcatc cacgagaacc tctatgtggg
     3961 cggcaccgat gcggagttcc atatctccat cagagaggat gctcccccgg gtgtcttctc
     4021 cgtggaggtc atcgccggat acggcgcccg gcgggcggcg ttcaaggtct tcgtccgcga
     4081 gcggaaagcc cccgagcccg ggccggttgc tgccccccgg gcggagcccg cccggactgt
     4141 atcgctcccg cctctcccgg ttctcgcccc cgtcgtcgcc gccctggtgc tctacggcct
     4201 ctggcttgca tcagggatcg acctccttaa tgccgccgcg tttgcggtgc tcctcgtcgg
     4261 ggtcatcctc gcatggctgc ggcagcggtc gtagtcgccg cgtcgctcct ggttgaccgg
     4321 ctggtcgggg atccgcactc ccgataccac ccggtggcgc tcctcggccg gttcatcggg
     4381 tggtgggggg tcccgtcccg ctacccggcc cgtgtccagc gggcggccgg ggccgcgggg
     4441 accattctga ccgtggcact ctttgccgcc ccgtttgccc tcgtccagtt cgccgccccc
     4501 tggtacctct ccatcatcct cgcgcccttc ctcctcaagg cctgtttcgc ctggcgatcg
     4561 cttgaggagc ataccgttgc cgtggtgcag gcgctcgagg acggcggcat cgacgccggg
     4621 cgcgcccggg ccggcatgct ggtgagcagg gatacggcgc acctcgaacc cgagcacatc
     4681 ctctcggctg cgtacgaatc gatgaccgag aacctggtcg atagcatcat ctccccgctc
     4741 ttctacttcg gattcttcgg cctcgcaggg gccgcggctt tccgggcgat caacaccatg
     4801 gacgcgatgc tcggttaccg cgacgagcgg ctccggatcg ggtggttccc ggcccgcgcc
     4861 gacgacattg caaacttcat accggcgcgc atcaccggtc tcgtcctcct cgtctatttc
     4921 gccgcaaagg ggcggttttc cccggcctac gcggtgctct gccgggatag aaagaagcgc
     4981 cccgggttca acggtggtat accgatggcc gtcatcgccg gcggcgtcgg cgtccggctc
     5041 gagaaaccgg gcgtctacgc aatcggggat gcggagcgga cacttaccga ggcgggcgcc
     5101 gggatcgtct ctgcggtccg ggcggcgacg atcatcttcg cccttgccga gatcggcgca
     5161 ttatttttat tggtatccat gcgctataca tagaggtaat gaaacttgag gaactgagat
     5221 ttggcaccga gctgatcaag cgcggctttg cgtcgatgca gagaggcggc gtcatcatgg
     5281 atgtcgtcaa cgcagagcag gcagagctcg ccgaaggggt cggtgccgtt gccgtcatgg
     5341 cgctcgagag ggtccctgcc gatatcaggg cggcaggggg tgtggcccgc atggccgacc
     5401 cgcagaagat cgtcgagatc atcgatgcgg tctccatccc ggtgatggga aaggcccgga
     5461 tcggccattt cgtcgaggca caggttctgg aagcgctcgg cgtggacatg gtcgacgaga
     5521 gcgaggtgct gaccccggcg gacgagcagt tccacatcga taagacccgg tttgaggtcc
     5581 cgttcgtctg cggtgcgagg aacctcgggg aggcgttgcg ccggatcgac gagggggcag
     5641 cgatgatccg gacgaagggc gaggccggga cggggaacgt cgtggaagcg gtccgccaca
     5701 tgcgggcgat catgggcggg atccggagcc tgaaagggct ctcttcccag gagctcatcg
     5761 accgggcccg cgagatcgag gcgccggcag aactcgtcat cgagtgcgca aaccttggcc
     5821 gccttcccgt ggtgaacttc tcggccggcg gcatcgcgac gcccgcggat gcggcgctca
     5881 tgatgcagct cggggccgac ggtgtctttg tcgggtcggg gatcttcaag tcgtcaaacc
     5941 ccgaagcgac cgcccgggcg atcgtggagg cggtgaacca ctacgacgac gcaaaggtca
     6001 tcgccgaggt gagccggggg ctcggggacg cgatgaaggg catcgatatc cgcgtgctcc
     6061 gggaagacga gatcctccag acgcgcgggc ggacctgaga cctcataccg gtgcgtgctg
     6121 caaagatctg gtgcggtact cgttctccgg cgccatgcca cttccccgtg gtggtcgata
     6181 tcgcggcgtt tgccgggttc accctggtct ttaccgccgc gatgcacctg cacaggcgga
     6241 gcatgccgcg gaggatgtga tcgcagcggc aggaagatgt ctcatcgatg ccaatagtgg
     6301 aggtgcacca tgaagaagga actcatcatc gaatggagac atgccggaag cgcggtcaac
     6361 aagtccccgg gaggggttga ggagaccggt atgactcttg cggccgttct cgcggagatc
     6421 cgcatgctcc ttgagatgga gggcgtcacc gtccggatga tcgagacgga tgcagaaccg
     6481 gagggcctcc tcttcaacgg cattccggtc gagaggctcc tcgaagggat cgaggtgact
     6541 gccctatcct gctcctgcgc atcctgcgac tcctgtgagg gagacgcgga gtgccgggcg
     6601 ctccgttaca acggcgaggc gtacgaggcg atcccgccgg acctgatagg ccgggcggcc
     6661 gtaaaagcgc ttgaggtgga gtgagggcgt ggcggagccc gccacccccg tcacctgctc
     6721 cggcctctca aagaccggga agctgaccgg ccgtgtgggg atggcggttc taacggcgcc
     6781 ttttgggctc cattgaggct ccgcctggag aagcgacggg aattgtgcga ttcaagagca
     6841 gaagcgcatg ggtgcatccc cggtcacgga tctccaccgt tcagggtaga gtttttcaac
     6901 cggttttcaa tgtaatatgc ttaatattaa cataatgtta aatgactatt tttaataagg
     6961 gccgccccca accacgatac cataagcctc cgatagctcc ccggagcgta tcggagaaga
     7021 ctgaatggac gtggtagtta ttacaaaacg ctgtcatctt ctgctcatca tgctcggtgt
     7081 ctgtgccgtc ctcctctgcg cctccgccgc ctgcacaacc gcccgggaat catctccttc
     7141 ggccgaagac cgcacgatca ccgatatggt ggaccggacc gtcgttatcc cgtcggagat
     7201 cgagagtgtt ctctgcacct cgccgccgtc gacaaacctc gtctacatgg tcgcgcccga
     7261 ccggcttaaa ggctggaact tcgcgcccga gaagggatac accgccgaga agtacatggc
     7321 gctcccggtc atcgggggct ggttcgggaa agagaccggc aactacgaga cgttcatatc
     7381 gatgcacccc gacatcgtca tcgagggttt caccaccgac ggcggagcgg cgagtgcctc
     7441 cgttgccgag cggcaacaga agatggaacc catcccggtc gtcgctgtcg aggatagcac
     7501 caactccacc ggctactccg cgcccatcag gttcgtgggc gacctcctcg gcgaggagga
     7561 gcaggcggaa gcgatgatcg ccttctacga gggcgtgctt gccacggtgg cggagcgggc
     7621 ggcctcgatc cccgacgagg agcgggtggg ggtctactat gccgaggggc ccaggggact
     7681 cacgaccgat ccctccggtt cgtcccactc cgaactcatc gacctctgcg gcgggagaaa
     7741 cattgcagat tgtgcgataa cgcccgggat gggaatgacg gaggtctcga tggagcaggt
     7801 catcgcctgg gatccggatg tcattctggt cgagaatccg gggttctacg ccgcggtcgg
     7861 gaccgacccg gcctggcagg agatcaccgc cgtgaagacg ggtcgggtct atctcacccc
     7921 gcggactgcg ttctgctggt ttgaccgccc gccgggcatc aaccggatca tcgggattcc
     7981 ctggactgcc aaaaccctgt atcccgatct cttccgggat atggaccttg agagcctcgt
     8041 ccgggagtat catgaaatct tcctccacgt atcgctctcc gatgaccaga tccaccagat
     8101 cctgaacctc tgagggtgag aagatgggat acacgccagg caggttcgac cgggacggat
     8161 cgaagaatat gaaccggatc gcaaaaaccg tcttcgcacc ggtctacccg gtgatcgcag
     8221 aacagatcgt cgagcggtgc aagatatccc gcgggcggtg ccttgatatc ggcagcggcc
     8281 ccgggtcgct cgggatagcg cttgcgcggg cgagcgacct taccgtcacg ctctttgact
     8341 cgtcttcgga gatgcttgcc ctcgccgaag ggaacatccg ggaggccggc ctctccggcc
     8401 gcctctcgct gctttcaggc gacgtccacg atatcccgct tcccgcggga tccgtcgaca
     8461 tcgcggtcag ccgcggttcg atcttcttct gggaggacct cgcccgggcg ttctccgaga
     8521 tccaccgggt tctcgcaccc ggtgggaaga cctatatcgg gggcgggttc gggaccgccg
     8581 aactccggga cgcagttctg gccgcgatga cgaaggagaa cccggactgg aagaactttc
     8641 gggataaaaa cctcggcccg gagaaccggg agcgggttgt cggcgtcatc gagggcctcg
     8701 ggctccctca ccggatcgtc aacgatgact ccgggttctg ggtcatgatg gagaaggggc
     8761 gataggcgta tgcggtgctc catctgcgag aaccggtgtg tgatcgggcc cggaggggtc
     8821 gggcggtgca ggatgtacac aaacgagggc gggacgatcg tcgagcggta tcccgaccgc
     8881 tacatcgcgg tcatgccgat tgctatcgag acgatgccga tgtgccacta ctatccccgg
     8941 gagacgttcc tgcaggcgag cggcatcggg tgcaccttct cctgtggagg atgcatatcc
     9001 gagacggtcg ccctccacgc ggaggcggtc tccggggcac tcaagcatat cccgcccgac
     9061 gccctgatcc ggcgcgcccg ggaggagggg tgccgcggga tagcgttctg cctcaacgat
     9121 ccgaccgcca tgcaccggac gtttctcgcg gtggcccggg cggcgcgcga tgcggggctc
     9181 ctcgtcgggt gcgcgacgaa cggctactat acggaagaag ccctccatga tctcgcgcct
     9241 gccatagact tcgtaaacat cgggctgaaa ggagcatccg atgcgcgcta ccgtgcgtgc
     9301 ggagcggtct cggcagaacc ggctttccgg gcggtgaaga cccttcacga agcaggcgtt
     9361 cacgtcgagg tctcctgtat ctacgcccgc ggctatgacg acgagatccg cgctgttgcc
     9421 gcccggattg cggatatatc tcctgatatc ccgctcctcg tcatgcgatt cataccgttt
     9481 ggcgacgagt cgccggagag ggaaccgacg atcgcggagt cggagcggat ctgcgacgac
     9541 ctgcggcgga cactccggta tgtctacctc ttcaactccc ccgggaccgg gtacctcgat
     9601 acggtctgcc cgtcctgtgg ggagagggtc gtcgggcgag agttcttcgg gccgatgggg
     9661 gcgcggacga tcgcggttcc gccggacgcc cggtgccttt gcgggttctc gctccccgtt
     9721 ctcgggagca tcggaaagac cccgtacgcc gaacccggta tgatgggcgg ctaccggttc
     9781 accagggcgc ttgagatggt ccatgccgtt ctggtctgcc tcggcgtgga gtccgacgcc
     9841 gatctcgccc ggatctggac cggggtcata aaggacgact atatccggga ccttcacggg
     9901 aagatccaga cgatagacgg gtataccggc cttgtccggg atctcgggga gcggtccggc
     9961 cgccccgaag cggctgaggc gcttgccgcc tatatcggcg accgggtggc ggcggtctcg
    10021 tcggcagtag aagggtatcc ccggccccgg gtttactact cgatggggac gccgcttttt
    10081 gccctgaacg ccgaccggtt cgagatggac ctcgtggagg cggccggcgg cgaccccgtc
    10141 aaccgcagga tcgtccgggc cggaaagccg ggggtcaaca taaccccgga agagttcgcc
    10201 gcgttcaacc cggagtacat attcatatcc gggttcctct ccgcaccggc ctccgattac
    10261 cttacggcat gccggaggat gggactctct gccgatgcga tcgagcacgg ccgggtctac
    10321 acccttccgc cgggctggga tttcggcaac ccccgctggg tgctcggcct ctctgccatc
    10381 gcgggcaccc tccacccggc gtgtgcggcg ttcgatcttg ctgcagagga ggatcggttc
    10441 taccggacgt tttacggggc cgaggcgagc ataaggaccg gaaacaggtc gttttaccgc
    10501 ccataagcac acccgcgcat ctccgccgcc ggttcgtcgg aggtgcgcct gcgggccgtg
    10561 ccgggagcga tgtagctctc cctgctaccc gggaacgccg cacgagttta tcgggatgca
    10621 ggccatccgc cccgcctcct cgatgtagcg gatgcagacg tcgaccccat aggctttgta
    10681 caggttctct ctcgtgatga cgtcctcggc cggcccctgc gcgatcagcc tgccgttctc
    10741 gaggatggcg accgtatgcg aggtcatgaa cccgtggtcc gggaagtggg tgctcatcag
    10801 gatgccgatc ccttcggcgg cgagccggtt gatcgtcgcg atcatcttca tctggttgcc
    10861 gatatcgaga tgcgatgtcg gttcgtcgag aacgagcagc gatggctcct gggcgagggc
    10921 gcgggcgatc atcaccatct gcgactctcc gcccgacatc tccggaagag gtttctcccg
    10981 gagatgggtg ataccgatcc gggcaaacgt ttcttccacc ttcctgtagt cctcctcttt
    11041 cgggaccgag aaggtcgaga tatggggggc ccttcccatg aggacgaagt cgagcgccgt
    11101 gaatggaaag gcggcgcggt ggacctgggg gacgtagccg acctctcgag cgacctcatc
    11161 gggcctcatc ccggcgatcc gtcgcccgtt gatctccacc ttcccgctct ggagcgagag
    11221 gatgttgaga atgcatttga tcagggttga ctttcccgta ccgttcttcc cgaggagaga
    11281 gagcacctcg cctctccgga gggagaagga gatatcttca aactgttttg tgatgccgtc
    11341 gtacgagaac gacgcatgct cgacgttcag gatcatgacc agccaactct ctttcgcgtt
    11401 aagaggtatg cgaagaatgg tgcgccgatg agcgccgtca ggaccccgag ggggatctcg
    11461 gctgcggtca gggtccgtgc gatgtcgtcg acgacgagaa ggaacgaggc cccgagtacg
    11521 gccgatacgg ggatgagcct ccggaaatcg gggccgacga gcatcctccc tatgtggggg
    11581 acgacgaggc ccacccagcc gatgatcccg ctgatgcaga ccgcggaggc ggtgacgacg
    11641 gtcgaggcga cgatgatcgc cgcggtcatc ttcttcgtat ccaccccgag tgcctgagcc
    11701 tcctcttcgc cgacggagag gaggttgatc cgccaccgga taaggaagag gcagagaccc
    11761 ccgaggagta tcggcggagc gactgcgagg agatcggcgt ttgaaactgc cgagaggctc
    11821 cccatcaacc agaagacgat cgcgggcaga gtgtcgtagg gatcggcgac gtatttggtg
    11881 agggagagga gcgcggagaa gacggacccg acgacgatcc ccccgaggac gaggatgagg
    11941 gtcgaggagt tccggtagac acggctgatg ccgtaggtga cggcgaccgc gacgatcccg
    12001 aagatgaaag cagagacctg gatgacccgg gggtctccgg agatgaggat cccgagtgcc
    12061 gccccgaacc cggcgcctga tgccacgccg aggatatcgg gggagacgag gggatttctg
    12121 aagaggccct ggaacgaggc tcccgcaagg gcgagccctc ccccgacaag cattgcagct
    12181 atcacccgcg ggagcctgat ctggaagatg accgtctgct ccgatgcccc ccaggcctgt
    12241 tcgaaggagg ggaggagaac ctgatgaccg gcaaaggtcg agagcaggga cgacgcagta
    12301 tcgaggaaga tggttgccga cccgacgatg aggattcgga gggtctccac cgggtccatc
    12361 atatacctcc cgaaaaagag tgaaaagacg aagaggacga ttggaaggat gtagatcgcc
    12421 ttctcgaccg gaaagatccg gagtacggcc tcgtagaccg gcataccgcc acgagaggca
    12481 acccacggga gttcgatggc gatagtcccc tcaggcctct gcccgacttc cacggtcggt
    12541 tcttccccac gggtcatccg gcacaccagt ccatacccaa tatgtatacc tgttttattt
    12601 aattttatgg aaatgtaaat ataataacat ttacgggggt ggtaaaaagg tttgggtgaa
    12661 ctgccctgca ccttgtgggc gggaaattcc cgttccgccc ctttcatccc caacagttaa
    12721 aatcagttcc tggcgtgacg gaaccccctc ggcgttctgg tgggcactcc tatgactgtt
    12781 gccccgggtg ccaaacccgg tctctctcag gatcaccggg ttatccggac agcaagcgtg
    12841 atggtggcct gtccgggctt atgcgttcac gagaacagaa tggtatgagc gatgtttcgg
    12901 cagcggccgt tgccatcgtt gtttttctgg cctggatccg gtgtaaatct tcacccttcc
    12961 gggccatagc gccgccggaa gattgacggg gcgcccgggg aggcgccggc ggccgcatcc
    13021 gcgagcaacg gaggaccctc ggcgatgcgg cccggggaga agggggagac cgggaccgca
    13081 gatgccgtct gcgcctcccg ccactcccag acggcatcca tcacgtcgcc tgccgtcgcc
    13141 atcacgcttg cgctcctgac gtccaccacg acggggtttg tgatcttcca gcgctcgatg
    13201 accgtatcga ggagccggcg ccggacggtc cagaccatcg gcggatgcga aagcgagacc
    13261 agccggtcga tctggtcggc ccagaccgcg ccctggagct cgaaccgtcc gacctcgtcg
    13321 atggccatca gatcggtctc ccgggggttc ggcggcgcga gcgcccggcg gccgaatgca
    13381 agcccttcgg ggcggaagta gaatcggccg tggacttcac accgcgggtc ggggtcgact
    13441 gaagagagtt cctcgtgttc gccggtggcg aggtcgacca gggtgaatcc cgacctccgg
    13501 ccgtcccgca cgtgcccggg agcgagcaca ccccggatcc ggacatttgc cccgacaatg
    13561 agcccgagga ccaggtgcaa aaacgtcgtc ttgcactggc cctgctcgcc ggttatgatg
    13621 aagaccggcg cccgaccgtt tgtaccggcg ggctcttgcc gcggcgggag gctgaatggg
    13681 gtgatgcggg tatatcccgg ccgcgggtcg gccgtgatct tcgctccggg gtacatgcat
    13741 atacttctgc ggccgcccat ataaaacatc tttacatatg ctatttgttt aggtaatacc
    13801 attgacgtga tccgccggga agggtgtatg cccgcccccg ggccggatgg cgatcttcac
    13861 ctgccggcgg tggggcatac gcctcccacc agtataagta acaacggcga acaataccat
    13921 acggagttat accaatggca gagaagaccg tcgtgcttca cacgaccatg ggcaagatcg
    13981 cgatccgcct ctacggcgac atgccggtga cggcgggaaa ttttgcaaaa ctggcaggat
    14041 ccgggtttta tgatggtaca atcctccacc gggtcatcgc gaatttcatg atccagggcg
    14101 gcgacccgac cggcaccggg atgggcgggc cgggctacac catcgccgac gagttcgtga
    14161 agggccattc aaaccgccgg ggcacgatcg ccatggcaaa caccggccgc ccgaacaccg
    14221 gcggcagcca gttcttcatc aacctggtgg acaacaccta cctcgactgg gacgaccccc
    14281 ggacgccgag cgcccacccg gtcttcggcg aggtggtcga ggggatggat atcgtcgata
    14341 agatcgggaa ggtaaggacg gactccgccg ataa
//
LOCUS       DAOSEE010000049        13924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1723_length_13924_cov_248.543875, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000049 DAOSEE010000000
VERSION     DAOSEE010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 13924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13924
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1723_length_13924_cov_248.543875"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..1288)
                     /gene="hmgA"
                     /locus_tag="PLI76_05245"
     CDS             complement(80..1288)
                     /gene="hmgA"
                     /locus_tag="PLI76_05245"
                     /EC_number="1.1.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase (NADPH)"
                     /protein_id="HOI61470.1"
                     /translation="MDEYIERLKDGSLKLYALEKELPPAEAVRVRRAFIEGETHTALT
                     GVGSFSIDIDRVVKANIENMIGTVQVPLGVAGPLRVKGEYATGSYYLPLATTEGALVA
                     SVNRGCSLITRAGGADVRIMRDGMTRAPVFAARDVGHAHGVAAWVDEHIEDIRRAAES
                     TTKHGKFLDAATYVAGTSVYVRLEFDTKDAMGMNMVTIASQKVGELIERETGARLIAL
                     SGNMCTDKKPAAINLVRGRGKTVVAGVRLTDAMIADLLKTDADTLAEANYRKNLVGSA
                     RAASLGFNAHAANVVAAMFIACGQDPAHVVEGSSAITTVDRVDGGVYVSVTLPSLPVG
                     TVGGGTGIDTQRECLTMLGVAGGGDPPGTHAKAFAEIVAAGVLAGELSLLGALAAQHL
                     ARAHQEHGRG"
     gene            1332..2246
                     /locus_tag="PLI76_05250"
     CDS             1332..2246
                     /locus_tag="PLI76_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOI61471.1"
                     /translation="MDAEQWCLVVEKREAEQTRRRLLEEGLLDRRYKPRSEGDAVLLP
                     VTEEIAGTTRRTFVTVPQRADLPRHDLVGGIAILQEDDPEAAKRLLASRPSLHTVLFP
                     ETPVEGNYRTRRFAVLAGTPTTRTRVTEYGLRFDVDLAHAYFSARLSTERQRILGMME
                     EGERVLDMFAGIGPFAITLARRAELVVAADLNPAAIHLLIQNIALNRTGNVIPALADA
                     AHLPRIGLAPFDRVVMNLPLAAPAFLPAAAALCREGGTIHLYALEEREGEYLPHIRAV
                     TAGEVVERQVRTYSPGKWHAVYDIEKEG"
     gene            complement(2313..2705)
                     /locus_tag="PLI76_05255"
     CDS             complement(2313..2705)
                     /locus_tag="PLI76_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HOI61472.1"
                     /translation="MSVSRFWRKIPQRYNLVGTECTTCGRYFFPPRSLCPDCRRSGHI
                     VDHKFNGEGTVVTYTVIRSASDQFEHTTPYVLAVVELDEGPRLTTQIACSPEEAKIGM
                     RVKSVFRRVAADGESGTIHYGTKFVPAE"
     gene            complement(2708..3868)
                     /locus_tag="PLI76_05260"
     CDS             complement(2708..3868)
                     /locus_tag="PLI76_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HOI61473.1"
                     /translation="MRDVAVIGVGCTEFGEHWSTSFRDLFVTAGALALEDAGISGEKI
                     DALYVGNMSAGRFVEQEHIGALIADYAGLASNHVPSTRVEAACASGGLAFRQAVISVA
                     SGMEDIVVAAGVEKMTDVGTGASVDMLASAADREWEGFAGATFPGLYAMIANDYMHRY
                     PLTREQLAAVAVKNHENGAKNPIAQFRNRITIDTVLSSSMVADPLRLFDCSPITDGAA
                     AVVVVPLERAREFTDSPVRVLASAQASDTIALHDRRDISTLDATVAAGKRAFAQAGLA
                     QKDIDLVEVHDCFTIAEICAIEDLGFCKKGEAGRLTAEGATALNGDIPVNTSGGLKAC
                     GHPVGATGIKQICEVVTQLRGDAGARQVDAEIGMTHNVGGTGATVVVHILGA"
     gene            complement(3865..4917)
                     /locus_tag="PLI76_05265"
     CDS             complement(3865..4917)
                     /locus_tag="PLI76_05265"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HOI61474.1"
                     /translation="MVGIYSYGVYIPRYRIKVPEIARVWGANADDITRGLGVFEKSVP
                     DLDEDTATIAVEAARAALRRRKIDTGAIGAVYVGSESHPYAVKPTACTVGEAIGATPN
                     MTAADYEFACKAGTAGLQTCMGLVRSKMIPFGIAIGADVAQGAPGDALEYTAAAGGAA
                     FVVGEGETIADINRTCSFTTDTPDFWRREGQSYPRHGGRFTGDPGYFKHVQGAARLMF
                     DAMGTGPKDYDYAVFHQPNAKFPQRVAKMLGFTDAQIRPGLVVPKLGNTYSGSSMIGL
                     AATLDVAKPGERIFVTSFGSGAGSDAFDITVTDVIESEAFDRAAAPAVEKLLANPIYL
                     DYALYAKHKGKIVMQK"
     gene            complement(4911..5621)
                     /locus_tag="PLI76_05270"
     CDS             complement(4911..5621)
                     /locus_tag="PLI76_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOI61475.1"
                     /translation="MKSGVRHQLAEKMAGEITLSDSPGQALKKWRQSFNIPQGLLAER
                     LEVSPSVISDYESGRRKSPGTAIVGKIVDAILSIDEDSGGRFINKFAKILYIEFDEDV
                     IYDIHEYASPVSLSDFAGAIDAATLCGPMDQTIYGYTVINSLNAILQLSSSEFNRIYG
                     WSTERALIFTEVSTGKSPMVAIRVTPFKPRCVVLQGINPEEVHPLIPGLAERDRITVL
                     CTTMDVEAIISALRGKLW"
     gene            5744..6412
                     /locus_tag="PLI76_05275"
     CDS             5744..6412
                     /locus_tag="PLI76_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF120 domain-containing protein"
                     /protein_id="HOI61476.1"
                     /translation="MVEAEDLQSLKMIALLGGCQGPIWLSSQSLASALGISPQTASRR
                     LIALERQQFVVRSMKPDGQYVAITRAGEQTLRREYADYVRIFDPERRQYVLTGTVISG
                     LGEGRYYMSLPHYKEQFSGSLGFEPFPGTLNIRLDAASVQVRKHLDHAGWIDIPGFTA
                     DERTFGGARVLPCRIRGEVCAIVEPSRTHYPEDIIEVIAGHELRKTLDLNDNDTIEVE
                     ITHD"
     gene            6405..7082
                     /gene="ribB"
                     /locus_tag="PLI76_05280"
     CDS             6405..7082
                     /gene="ribB"
                     /locus_tag="PLI76_05280"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HOI61477.1"
                     /translation="MIDAAIQALARGEFVLLYDFDNRERETDFAIRSDAVTPAHIRQM
                     RKDGGGLICTAVHPDAAKRLGLPFAHDLLRACSLVEKDGEVPYDRKNHSSFSLWVNHR
                     ETYTGITDRDRALTVTAIAEEVKRSLNGGGHDFAAHFRTPGHMALLRAADRLLDERRG
                     QTELSIALAAMADVTPAVTICEMLDDDTGFALSKEGAMEYARKHGLVFVEGQDVLDRW
                     ESQKQGR"
     gene            7191..7835
                     /locus_tag="PLI76_05285"
     CDS             7191..7835
                     /locus_tag="PLI76_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOI61478.1"
                     /translation="MKRDAVAYLNTRRKGDFAAQKDEVENYCKYRFQIAAIFHDHRAG
                     ATHPERREGFLAMLEYCAANDCPEIIIYDLADLAKDMDTGLAALKTLNEDYTVYCVNN
                     DFFGSRDDPALRREAIGNFIRYMGRYREGTGKAAAPAPRKAKKEGGQAIGRPRALSEG
                     QIEALITVRRAGTSIGKICRMFDVSRSTVSKILADRPELKGEWKGARQEPPEEV"
     gene            complement(8192..8437)
                     /locus_tag="PLI76_05290"
     CDS             complement(8192..8437)
                     /locus_tag="PLI76_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61479.1"
                     /translation="MSEMTSIKISVEVKNQLTFRYNGCDTGIRTWVSHGTKEISDQLL
                     SRMAEQLNLSRQQFPDLIDCKLDEPGLIAIYEEMRLL"
     gene            complement(8617..9348)
                     /locus_tag="PLI76_05295"
     CDS             complement(8617..9348)
                     /locus_tag="PLI76_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
                     /protein_id="HOI61480.1"
                     /translation="MDRVVFNPFAPLMLILLFGLLVLVGLVIILFPIVFLTAIGATFT
                     KLGFSWREALLILLLTLAGSFVNVPVRTLESRAAPAYDRYVAVYGRLYRIPEPVQRTV
                     LAVNVGGALIPLAISLYLLYDSVVISGGYLVMGLALAGVAVVTVVTKLVARPVPGLGI
                     ATPFFIPPLAALIAALVLSTFAGEVPAAAVIIAYVSGTLGTLIGADLLNLGHIAELGA
                     PMASIGGAGTFDGIFLSGIIAALLA"
     gene            complement(9378..9980)
                     /locus_tag="PLI76_05300"
     CDS             complement(9378..9980)
                     /locus_tag="PLI76_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrillarin-like rRNA/tRNA
                     2'-O-methyltransferase"
                     /protein_id="HOI61481.1"
                     /translation="MIRLGNVLVSPGEGGVYGERTLDGYRVWDPYRSKLAALYTLGGG
                     VDLTPDMRVLYLGAANGTTVSHVADYAGTVYAVEFAPRPMQDLLEVARRRKKIVPIMA
                     DAGRPEEYAPFMEAVDLIYQDVAQPDQVGIAERNLVFLKPGGHFILMLKTRSVDVRRD
                     PVEVLGEARAGLGRRLDVVDVRWLEPYHHDHAAIVCSRRG"
     gene            complement(9977..10837)
                     /locus_tag="PLI76_05305"
     CDS             complement(9977..10837)
                     /locus_tag="PLI76_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-processing protein"
                     /protein_id="HOI61482.1"
                     /translation="MLQRYWFGDVDEQGCRAAGTDPAALAERAAALRTGMESYVPIDW
                     EVARDCGVARTREEYVDLLRAVCITLAREKIAVSYRARDVELLQMVRMLDELDNVINL
                     LSERSAEWYQVTTPSFSRKYRSLPAKKMLDLVRRGARGGLSDVAGDIERLAGTRSRLM
                     REVSARADEVLPNTSALIGGLVAARLLSRAGGLSALARMPGSAIQVLGSERALFSHLR
                     GGTPPPKHGIIFQHRRVHNAPREVRGRVARVLAAKLAIAARLDYYRGEAVPEFIRDAQ
                     EKIDGAGVAA"
     gene            complement(10871..12088)
                     /locus_tag="PLI76_05310"
     CDS             complement(10871..12088)
                     /locus_tag="PLI76_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOI61483.1"
                     /translation="MSVLIGIVFGAVIGIGCGAISGLVPGIHANTMAGLLLSLQALLL
                     AWFEPVVIASAMFAALVTHTFVDCVPGTFLGIPDADTSLAVLPAHSLCLDGRGEEAVR
                     ISALGSAAGAALSLPLAAAFVLVLPALQPGIDWGIGLIILAVGGYLIVVSESPGWALA
                     VFSVSGMLGLFSLNYSFLAWPTGGESGVLMPLLSGLFGIAVLLRASHGLMPAQHFTGI
                     ELLPRAIRRGSILGSLAGALVGWLPGLSNATANALLTSVIGYDSNPREYILATSAANT
                     VNAFLGLAAFYAISRTRNGVMAALAAAEEIPPATALLLAGALAAVGAYLLTLLLSRAA
                     WLFGDVNVTRLNQGVIAFVVLLSLFLCGPFGGLVLVLATAVGCVPSLVNIRRVYCMGA
                     IMVPVMAYSFGLA"
     gene            12171..12845
                     /locus_tag="PLI76_05315"
     CDS             12171..12845
                     /locus_tag="PLI76_05315"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOI61484.1"
                     /translation="MKIAITRLENKAAGDRALCATYGHDCYTVSPLRAELRMERVAAF
                     VDAVHHSEFDCLFFTSALPAGVVAPRLKRWPRVIAIGPKTAEILRESGIDAEVLPSFY
                     SRDFVPYLGDWLRGRTVGIPRADVPNPGLLDGIVAAGGKYHEERIYALVPTGTDLALD
                     AADAVLFTSAMSYREARWHPRVDLLLVAIGEITADAMRAGGHPPAVVGDGSLAGTLEA
                     LNREGV"
     gene            12842..13756
                     /locus_tag="PLI76_05320"
     CDS             12842..13756
                     /locus_tag="PLI76_05320"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided pseudouridylation complex
                     pseudouridine synthase subunit Cbf5"
                     /protein_id="HOI61485.1"
                     /translation="MTGREETPVPASGIVVIDKPRGPSSHQVTAWAGEILGRRVGHAG
                     TLDPEVSGVLVVMFGGAVRLAPVLLSHNKEYVCLMRLHGDVPGEAVGRVAEEFTGRVY
                     QRPPKKSAVKRNLRIRKIQEIEVLDREGRLVLLRVRCDAGTYIRTLCIHMGYALGVCA
                     HMQELRRIRSGPFDETAAVSLHELADAVIASREGDPGPLRDMIRPPEATVVDLAKVVL
                     RDTAVDAVCHGAVLAGVGIAGKEGGFAKGETVAIMTGRGELVGLGKALVGSSALKPGE
                     PGFVVAPTTILMQPGTYPRGWKTHAREP"
     gene            13804..13890
                     /locus_tag="PLI76_05325"
     tRNA            13804..13890
                     /locus_tag="PLI76_05325"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13838..13840,aa:Ser,seq:gga)
BASE COUNT         2529 a         4488 c         4514 g         2393 t
ORIGIN      
        1 cttccggatc gcatcgtatt acgcgaacgg tgaagatgcc cttatcatga tgaagtggtt
       61 tcggttttaa tggggagggt tatccccgcc cgtgctcctg gtgggcccgg gccaggtgct
      121 gcgccgcgag ggcgccgagg agggagagtt cgccggcaag gactcccgcg gcgacgatct
      181 ccgcaaacgc cttcgcgtgg gttcccggtg gatctccgcc cccggcaacg ccgagcatcg
      241 tgagacattc cctctgggtg tcgatccccg tgccgccgcc gaccgtcccc acgggaagag
      301 aggggagggt gaccgagacg tagaccccgc cgtccacccg gtcgacggtg gtgatcgcgc
      361 tgctcccctc gacgacgtgg gccgggtcct gcccgcaggc gatgaacatg gcggcaacga
      421 catttgccgc atgggcgttg aacccgagcg aggccgcccg cgcagaaccg accaggttct
      481 ttctgtagtt ggcttcagcg agcgtatccg cgtcggtctt caagaggtcg gcgatcatcg
      541 cgtcggtcag gcgcacgccg gcaaccacgg tcttcccccg gccccggacc aggttgatcg
      601 ccgccggctt tttgtcggtg cacatgttcc ccgagagggc gatgaggcgg gcgccggtct
      661 cccgctcgat gagttcgccg accttctggc ttgcgatcgt caccatgttc atgcccatgg
      721 cgtctttggt gtcgaactcg agccggacgt agacgctcgt gccggcaacg taggtggcgg
      781 cgtcgaggaa cttcccgtgt ttcgtggtgc tctccgcagc tctccggatg tcctcgatat
      841 gctcgtccac ccaggccgcc acaccgtggg cgtggccgac gtcgcgggcg gcaaagaccg
      901 gcgcacgggt catgccgtcc cgcatgatcc ggacatccgc gcctcccgcc cgggtgatga
      961 gagagcaccc gcggttcacc gatgcgacga gcgccccctc cgtcgtcgcg aggggaaggt
     1021 agtaggaccc ggtagcgtac tctcccttca ccctgagcgg gcccgcaacg ccgagcggca
     1081 cctgcaccgt gccgatcatg ttctcgatgt tagccttcac cacccggtcg atatcgatgg
     1141 aaaacgaccc gaccccggta agggcggtgt gagtctcgcc ctcgatgaac gcccgccgca
     1201 ccctgaccgc ctcggcgggc gggagttcct tctcaagcgc gtagagcttg agagagccgt
     1261 ctttgagcct ttcgatatac tcatccatga atagtatgag aacgcatacc taaagatggc
     1321 tggtggtcga tgtggacgcg gaacagtggt gccttgttgt agagaagcgg gaagccgagc
     1381 agacacggcg ccggctgctt gaggaggggc ttcttgaccg gcggtataaa ccgcgctccg
     1441 aaggggatgc ggtcctcctc ccggtgacgg aggagatcgc gggtactacc cggcgcacct
     1501 ttgtaaccgt gccacagcgc gccgatctcc cccgccacga cctcgtgggc gggatcgcta
     1561 tcctgcagga agacgatccg gaggcggcaa aacggctgct tgcatctcgc ccttcccttc
     1621 acacggtcct cttcccggag acaccggtcg aggggaatta ccgcactcgc cggttcgccg
     1681 tcctcgccgg gactcctacc acacgcaccc gggtgacgga gtacggcctc cggttcgacg
     1741 tggaccttgc ccacgcctac ttctcggccc gtctctcgac cgaacggcag cggatcctcg
     1801 ggatgatgga ggagggggag cgggtgctcg acatgttcgc cggcatcggg ccgtttgcaa
     1861 tcaccctcgc ccggcgggcg gagctcgtcg tcgccgcgga cctaaacccc gccgcaatcc
     1921 acctcctcat ccagaacatc gccctcaacc ggacaggaaa cgtcatcccg gcgctcgcgg
     1981 atgccgcgca tcttccccgg atcggcctcg cccccttcga ccgggtcgtc atgaatctcc
     2041 cgctcgcagc accggcattc ctcccggcgg ccgccgcact ctgccgggag ggggggacga
     2101 tccacctcta tgccctggaa gagcgggagg gcgagtacct cccccatatc cgggcggtta
     2161 ccgcgggaga ggtcgtcgag cggcaggtcc ggacatactc gccggggaag tggcacgcgg
     2221 tatacgatat agaaaaagag ggatagcggg gggtcccggg tagacgggta tagagcgtga
     2281 tccggttttc gcgcgagatc ccccggtggt aattactccg ccggcacgaa cttcgtaccg
     2341 tagtggatgg taccgctctc gccgtccgcc gccacccggc ggaagacgct cttcacccgc
     2401 atgccgatct ttgcctcttc gggcgagcag gcgatctggg tcgtgagcct cggcccctcg
     2461 tcgagctcga cgaccgcgag gacgtagggt gtggtgtgtt cgaactggtc gcttgccgac
     2521 cggatgaccg tgtaggtgac gaccgttcct tcgccgttga acttgtggtc gacgatatgg
     2581 ccgctcctcc ggcaatcggg gcagagcgac cgcggcggga agaagtaccg gccgcacgtc
     2641 gtgcattccg tcccgacgag gttataccgc tgcgggatct ttctccagaa tcgtgaaacc
     2701 gacattgtta cgcccccagg atgtgcacga cgaccgtcgc accggtgccg ccgacgttgt
     2761 gggtcatgcc gatctccgcg tccacctgcc gggccccggc atcgccgcgg agctgggtga
     2821 ccacctcgca gatctgcttg atgccggtcg cgccgacagg gtggccgcag gccttcaggc
     2881 cgccgctcgt gttcaccggt atatccccgt tgagagctgt tgccccttcg gcggtgagtc
     2941 ttcctgcctc gcccttcttg cagaacccga ggtcctcgat agcgcagatc tctgctatcg
     3001 tgaagcagtc gtggacctcg acgaggtcga tgtccttctg ggcgaggccc gcctgggcaa
     3061 acgcccgttt gcctgcggcg acggtggcat ccagggtgga tatgtcccgc cggtcgtgga
     3121 gtgcgatggt gtcgctcgcc tgggcgcttg cgagcacccg caccggcgag tcggtgaact
     3181 cgcgggcgcg ctcgagcggc accaccacga ccgccgccgc accgtcggtg atcggcgagc
     3241 aatcgaagag ccgcagggga tcggcgacca tcgatgagga gaggaccgtg tcgatcgtta
     3301 tccggttccg gaactgggcg atcgggttct ttgcgccgtt ctcgtggttc ttcaccgcca
     3361 ccgcggcaag ctgctcgcgg gtgagcgggt agcggtgcat gtagtcgttt gctatcatcg
     3421 cgtagaggcc ggggaaggtc gctccggcaa acccctccca ctcgcggtcc gcggcgctcg
     3481 cgagcatatc gacgctcgcc ccggtcccga catcggtcat cttctcgacg cccgctgcga
     3541 ccacgatatc ttccatgccg cttgcgaccg atatgaccgc ctgccggaag gcaagcccgc
     3601 cggatgcgca ggccgcctcc acccgggtcg aggggacgtg gttgctcgcc aaccctgcgt
     3661 aatccgcgat cagggccccg atgtgttcct gctcgacgaa ccggccggcg ctcatgttcc
     3721 cgacatacag ggcgtcgatc ttctcgcccg atatcccggc atcttcaagc gccagcgctc
     3781 ctgccgtcac gaagaggtcg cgaaacgacg tgctccagtg ttccccgaac tccgtgcacc
     3841 cgacaccgat cactgctacg tctctcattt ctgcatcact atctttcctt tgtgtttggc
     3901 atacagtgca tagtcgaggt agatcgggtt tgcgaggagt ttttccacgg ccggcgccgc
     3961 cgcccggtcg aatgcctccg actcgatcac gtcggtgacc gtgatatcga aggcgtcgct
     4021 ccccgccccg gaaccgaacg atgtgacgaa tatccgctcg cccggttttg cgacatcaag
     4081 cgtcgccgca agcccgatca tcgacgaccc cgagtaggtg ttgccgagct tcggaacgac
     4141 gaggccgggc cggatctgtg cgtcggtaaa gccgagcatc tttgccaccc gctgcgggaa
     4201 cttcgcgttc ggctggtgga agaccgcgta gtcgtagtcc ttcgggccgg tgcccatcgc
     4261 atcgaacata agccttgcgg cgccctggac gtgcttgaag tagccggggt cgccggtgaa
     4321 ccggccgccg tgccgggggt aactctgccc ctcgcgccgc cagaaatcgg gcgtatcggt
     4381 agtgaaggag caggtccggt tgatatcggc gatggtctcc ccttcgccga cgacgaacgc
     4441 ggccccgccg gccgccgccg tgtactcaag agcgtcgccg ggagcgccct gggccacatc
     4501 cgcaccgatg gcgatcccga acgggatcat cttgctccgg acaagcccca tacaggtctg
     4561 gaggcctgcg gttcccgcct tgcacgcgaa ctcgtaatct gcggcggtca tgttcggtgt
     4621 cgccccgatc gcctcgccga ccgtgcaggc ggtgggtttg accgcgtagg ggtgggactc
     4681 gctccccacg tagaccgcac cgatagcgcc ggtatcgatc ttcctccgcc ggagggcggc
     4741 acgggccgcc tcgacggcga tcgttgcggt atcttcgtcg agatcgggga cggacttctc
     4801 aaagaccccg aggccccggg tgatgtcgtc tgcgttggcg ccccataccc gcgcgatctc
     4861 cgggactttt atccggtatc gtgggatgta gacgccgtac gaataaatac ctaccataat
     4921 ttccctcgta atgcgctgat gatcgcctcg acatccatcg tggtacagag caccgtgata
     4981 cggtcgcgct ccgcaagacc cggtatcagc gggtgcacct cttcgggatt gatcccctgc
     5041 aggaccacac agcggggttt gaagggggtg acccggatag ccaccatggg tgacttcccg
     5101 gtcgagacct cggtgaatat gagcgcacgc tccgtgctcc agccgtatat acggttgaac
     5161 tcgcttgacg agagctggag gatcgcgttg aggctgttga tcaccgtgta cccgtagatg
     5221 gtctggtcca tggggccgca gagcgtggcg gcatcgatgg ctccggcaaa gtcggagaga
     5281 cttaccgggg atgcgtactc gtggatgtcg tagatgacgt cttcgtcaaa ttcgatgtac
     5341 agaatttttg caaatttgtt gatgaaccga ccgccgctgt cttcgtcgat cgagaggatc
     5401 gcgtcgacga tcttgccgac gatggcggtt cccggactct ttcgccgtcc actttcgtaa
     5461 tcgctgataa ctgagggaga gacctcgagc cgctcggcaa gcaacccctg agggatgttg
     5521 aaactctgcc gccacttctt caaggcctgt cccggtgagt ccgagagtgt gatctctccc
     5581 gccattttct cggcaagttg atgccgcact ccggatttca tgcagctaat gttagcaccg
     5641 gaacttatat aattagcgta tcccatatcg acatttcacg aaggcctgcc ggcatggtgt
     5701 cgttacaaaa cccgtatata gtatgcatct caattatgat ataatggttg aagcggaaga
     5761 tctgcagtcc ctgaagatga tcgccctgct cggcgggtgc cagggcccga tatggctctc
     5821 gtcgcagtcg ctcgcgagcg ctctcggcat cagcccccag accgcatcgc ggcgccttat
     5881 cgcgcttgag cggcagcagt tcgtcgtccg gtcgatgaag cccgacggcc agtatgtcgc
     5941 tatcacccgt gcgggcgagc agacgctgag gcgggagtat gcggactacg tgcggatctt
     6001 cgacccggag cgccggcagt atgtcctgac cgggaccgtt atcagcggcc tcggggaagg
     6061 gcgctactac atgagcctcc cccattacaa agagcagttc agcgggtctc tcgggtttga
     6121 gccctttccc ggcaccctga acataaggct cgatgcggcg agcgtccagg tcaggaagca
     6181 tctcgaccat gcaggatgga tcgatatacc cgggtttacc gccgatgaac ggacgttcgg
     6241 cggcgcccgg gtgctcccct gccgcatccg gggcgaggtg tgcgcgatcg tcgagccctc
     6301 ccgcacccat tacccggagg atatcatcga ggtcatcgcc ggccacgagc tccgcaagac
     6361 cctggacctg aacgacaacg atacgatcga ggtggagatt acccatgatt gatgcagcca
     6421 tacaggccct tgcacggggc gaatttgtcc tgctctacga ctttgacaat cgggagcgcg
     6481 agaccgactt tgcgatacgc tccgatgccg tcacgcccgc ccacatccgg cagatgcgta
     6541 aagacggcgg cggcctgatc tgcacggcgg tgcatcccga tgccgcaaag aggctcggcc
     6601 tcccgttcgc ccacgatctc ctccgggcct gcagcctggt cgagaaggac ggcgaggtcc
     6661 cgtacgaccg gaagaaccac tcgtcgttct ctctctgggt aaaccaccgc gagacctata
     6721 ccgggatcac ggaccgcgac cgcgccctga cggtcaccgc catcgccgaa gaggtgaagc
     6781 ggtcgctcaa cggcgggggg cacgattttg ccgcgcactt ccggacaccc ggtcacatgg
     6841 cgcttctacg ggccgccgac cggctgctcg acgagcggcg cggacagacc gagctctcga
     6901 tcgcgctcgc cgcaatggcc gatgtcactc ctgcggtcac gatctgcgag atgctggacg
     6961 acgatacggg gtttgcgctc tctaaggagg gagcgatgga gtatgcgagg aagcacggcc
     7021 tcgtctttgt cgaggggcag gatgtcctcg accggtggga gtcgcagaag cagggaaggt
     7081 gaaccttctt tctcctcagg ggttgctcgc cgttttgcgg gcggagggat gcggcggtag
     7141 cggcgcactt ttcataaatc atatatgcat tcctgcggca ccataaacgg atgaagagag
     7201 atgctgtggc atacctgaat acgagaagga aaggagattt cgcagcacaa aaggatgaag
     7261 tcgagaatta ctgcaaatat cggttccaga tcgccgcgat ctttcacgat caccgtgccg
     7321 gcgcaacgca tcctgagagg cgggaggggt ttctggcgat gctcgagtac tgcgccgcaa
     7381 acgactgccc cgagatcatc atatacgatc tggccgatct cgcaaaggat atggataccg
     7441 ggcttgcagc actcaagacc ctgaacgagg actacaccgt ctactgcgtg aataacgact
     7501 tttttgggtc tcgtgacgat ccggcgctcc ggagggaggc catcggcaac ttcatccgct
     7561 atatgggccg gtaccgggag ggcaccggga aggctgccgc cccggcaccg aggaaggcga
     7621 agaaagaagg ggggcaggcc atagggaggc cgcgggcgct cagcgagggt cagatcgagg
     7681 cgctcatcac ggtgcgccgg gccggaacaa gcatcggcaa gatctgcagg atgtttgatg
     7741 tgagcaggag cacggtcagc aagatcctcg ccgatcgtcc tgaactgaaa ggcgagtgga
     7801 agggggcgcg ccaggaacct ccagaggagg tctgattact gcacgatgaa ccgccgtgct
     7861 caagccggac ctctctcccg ggggtgacgc tcgcgccgaa caccttcacg aagtcggggt
     7921 cgagtttcag ggtgactcct cgatcttgcc cggcacgtca aaggcaccgt tccgcaggag
     7981 agtacagatc tcctcggccg gcatggtccc gcgctctttg agaagacctt cgggggcgct
     8041 caaatattgt tatcgatggc aggcgcatgt agagcaggtt ggaccgacac gctgcccggt
     8101 ggcgtgcgcg aaaataatct cggaacaacc ctccggtaaa accggggcga tcagagatcg
     8161 atgctctctc cgggggaaga aatgctataa ttcagaggag gcgcatctcc tcatatatcg
     8221 caatcaatcc cggttcatcg agtttgcagt cgatgagatc gggaaactgc tgccgggaga
     8281 gattgagctg ttctgccata cgagaaagca actggtcgct gatctccttt gtgccgtggg
     8341 agacccacgt acggattcct gtatcgcagc cgttgtatct gaatgttaat tgatttttta
     8401 cctccactga gatcttaatc gacgtcatct ctgacatact actgtgtacc acccggggag
     8461 tatataccag actcgatcac aggcatgagg tgcctgtgat atcggcgctg caggcaatgc
     8521 gcacttgaag gcgtcggatg agcgctgtcg agcctgtgga ttcgcttccg ccggggaagg
     8581 ggaataatag tcgggaagcc tcatccggcc gcctatctac gccaacagag ccgcgatgat
     8641 cccggagagg aagatcccgt caaacgtccc ggccccgccg atgctcgcca tcggcgcccc
     8701 cagttccgcg atatggccga ggttcagaag gtccgccccg atcagggtgc cgagcgtccc
     8761 gctcacgtag gcgatgatca cggccgcggc cggaacttcg cccgcaaacg tcgagaggac
     8821 gagcgccgcg atgagcgccg caagcggcgg tatgaagaac ggcgttgcga taccgaggcc
     8881 cggcaccggg cgggcgacga gcttcgtcac gacggtcacg accgccacgc cggcgagggc
     8941 gagccccatg accaggtagc ccccgctgat cacgaccgag tcgtagagga ggtagagcga
     9001 tatcgcgagc gggatgaggg cgccgccgac gttgaccgcg aggaccgtcc gctgcacagg
     9061 ctcggggatc cggtagagcc ggccgtacac ggcgacgtag cggtcgtacg ccggcgcggc
     9121 gcggctctcg agcgtcctga ccggtacgtt gacgaaactc ccggcaagcg tcaggagaag
     9181 gatcaggagc gcctcccgcc aggagaaccc gagtttcgtg aacgtcgccc cgatcgccgt
     9241 cagaaagacg atcggaaaga gaatgatgac gagcccgaca agtacgagca gcccgaagag
     9301 gagaatgagc atcaggggag caaaggggtt gaagacgacc cgatccatac gaagagttcg
     9361 cccccagcta ctatatatta tccccggcgg gagcagacga tcgcggcgtg gtcgtggtgg
     9421 tagggctcaa gccaccggac atccaccacg tcgaggcgtc gtccaaggcc ggcccgcgcc
     9481 tcgccgagca cttcgaccgg gtcccgccgg acatccacgc tccgggtctt gagcatcagt
     9541 ataaagtgcc ctccgggctt gaggaagacc aggtttcgct ccgctatccc gacctggtcc
     9601 ggctgcgcca cgtcctggta gatcaggtcg accgcctcca tgaacggcgc gtactcctcc
     9661 ggccggcctg cgtcggccat gatcgggacg atcttcttcc tccggcgggc cacctcgagg
     9721 aggtcctgca tcggccgggg tgcaaactcc actgcataga cggtcccggc gtagtcggcg
     9781 acgtgcgaga ccgtggtccc gttcgccgca ccgaggtaga gcaccctcat atcgggtgtg
     9841 agatccaccc cgccgccgag cgtatagagc gccgcaagct tgctccggta gggatcccag
     9901 acccggtagc cgtcgagcgt ccgctccccg tagaccccgc cctccccggg cgagacgaga
     9961 acgttcccga gtcggatcat gccgcgaccc ccgccccgtc gatcttttcc tgcgcatccc
    10021 tgatgaactc cggcaccgcc tccccccggt agtagtcgag ccgggcggcg atagcaagct
    10081 tcgccgcgag cacccgggcc acccttccgc ggacctcgcg gggcgcgttg tggacccggc
    10141 ggtgctggaa tatgatcccg tgcttcggcg gcggcgtccc gccccgcagg tgcgaaaaga
    10201 gcgcccgctc cgatccgagg acctggatgg cgctccccgg catcctcgcg agcgccgaaa
    10261 gtccccccgc ccgggagagg agacgggccg cgaccaggcc gccgatgagg gcgctcgtgt
    10321 tcggcagcac ctcgtcggcc cgggccgaga cctcccgcat caggcggctc ctcgtcccgg
    10381 caagccgctc gatatcgccg gcgacatccg aaaggccgcc ccgggcaccc cgccggacga
    10441 ggtcgagcat cttctttgcc gggaggctgc ggtacttccg cgagaacgac ggcgtcgtga
    10501 cctggtacca ctccgcagaa cgctccgaga ggaggttgat gacgttgtcg agctcgtcga
    10561 gcatcctgac catctggagg agctcgacat cccgggcccg gtaggagacc gcgatcttct
    10621 cccgggcaag cgtgatgcag accgcgcgga gcaggtcgac gtactcctcc cgggtccggg
    10681 cgaccccgca gtcccgggca acctcccagt cgatgggaac gtacgactcc ataccggtgc
    10741 ggagcgcggc cgcccgctct gcgagcgcgg ccggatcggt gccggccgcc cggcacccct
    10801 gctcgtcgac gtccccgaac cagtagcgct gtagcatggt atggtatagg aagttcgccg
    10861 atataagagg tcaggcaaga ccgaacgaat aggccatcac cgggaccatg atcgccccca
    10921 tgcagtagac ccggcggatg ttgacgagag acgggacgca gccgaccgcc gttgcaagca
    10981 cgaggaccag tcccccgaac ggcccgcaga ggaagaggga gagcaggacg acgaaggcga
    11041 tgacgccctg gttcaaccgc gtgacgttca cgtctccaaa caaccaggcc gcccgcgaga
    11101 ggaggagggt caggaggtag gccccgaccg cggccagtgc gcccgcaagg aggagggcgg
    11161 tggccggcgg gatctcctcc gccgccgcga gcgccgccat caccccgttt cgcgtccggg
    11221 atatggcgta gaacgccgca agcccgagga aggcgttcac ggtgttcgca gcgctggttg
    11281 caaggatata ctcgcgcggg ttggagtcgt agccgatgac cgatgtcagg agagcgtttg
    11341 cggtggcgtt cgagaggccg gggagccagc cgacgagagc gcccgcgaga gaaccgagga
    11401 tggagccgcg ccggattgcc cggggtaaga gttcgatccc cgtgaagtgc tgtgcgggca
    11461 tgagcccgtg cgacgcccgg aggaggaccg cgatcccgaa gagccccgag aggaggggca
    11521 tcagcacccc ggactcgccg cccgtcggcc aggcgaggaa ggagtagttg agggagaaga
    11581 gaccgagcat cccggagacc gagaagaccg cgagcgccca ccccggcgac tcggagacga
    11641 cgatgaggta acctccaacc gcgagtatga tgaggccgat gccccagtcg atccccggct
    11701 ggagcgcggg gaggaccagg acgaacgccg ccgcgagcgg gagagagaga gcggcgccag
    11761 cggcgctccc gagggccgat atcctgacgg cctcctcccc ccgcccatcg aggcagaggg
    11821 agtgcgcggg gaggaccgca agcgacgtat cggcatcggg gatgccgagg aacgtcccgg
    11881 ggacgcagtc gacgaacgta tgcgtgacga gcgcggcaaa catggccgac gcgatgacca
    11941 ccggttcgaa ccaggcgagc aggagcgcct ggagcgagag gagaaggccc gccatggtgt
    12001 ttgcatggat gccgggaacg aggccgctga tggcgccgca gccgatgccg atgacggccc
    12061 cgaagacgat accgatgagc acgctcataa tcgatcggaa ccgccgggga taaaccaacc
    12121 ggaattacac cggggataca gtcatatccc acgaggtgaa cgttataccg atgaagatcg
    12181 ccatcacccg gctcgagaac aaagctgcgg gagaccgtgc cctctgtgcc acctacggcc
    12241 acgactgcta cacggtctcg ccgctccgcg ccgagctccg gatggagagg gtcgccgcgt
    12301 tcgtcgatgc cgtccaccac agcgagttcg actgcctctt cttcacgagc gccctcccgg
    12361 ccggagtcgt cgcaccgcga ctcaaacggt ggccccgggt gatcgccatc gggccgaaga
    12421 ccgccgagat cctccgggag tcgggtatcg atgcggaagt tctcccctcg ttttactccc
    12481 gcgacttcgt cccctacctc ggcgactggc tccgggggcg gacggtcggg atcccgcggg
    12541 ccgacgtccc gaacccggga cttctcgacg ggatcgtcgc agcgggcggg aagtaccacg
    12601 aagagaggat ctacgccctc gtccccaccg ggaccgacct tgcgctcgat gccgccgacg
    12661 ccgtcctctt cacgagcgcc atgtcctacc gggaggcccg gtggcatccc cgcgtcgatc
    12721 tcctcctcgt cgccatcggc gagatcaccg ccgacgccat gcgggccggc gggcatcccc
    12781 cggcggtcgt cggggacgga tcgcttgcag gaaccctcga ggcgctgaac cgggaaggcg
    12841 tatgaccggc cgggaagaga cccctgtccc cgcctccggc atcgtcgtca tagataagcc
    12901 ccgggggccg agcagccacc aggtgaccgc ctgggccggg gagatactcg ggcggcgggt
    12961 gggtcacgcc ggaacgctcg acccggaggt ctccggggtg ctcgtcgtca tgttcggcgg
    13021 cgccgtccgg ctcgcccccg tcctcctctc gcacaacaag gagtatgtct gcctgatgcg
    13081 gctccacggc gacgtgccgg gagaggccgt cggccgggtg gcggaggagt ttaccggccg
    13141 ggtctaccag cggccgccca aaaaaagcgc cgtgaagcgg aacctccgga tccggaagat
    13201 ccaggagatc gaggtcctcg atagggaagg cagactcgtc ctcctccggg tccggtgcga
    13261 tgccggcacc tacatccgga cgctctgcat ccacatgggc tacgccctcg gggtctgtgc
    13321 gcacatgcag gaactccggc ggatacgctc cgggccgttc gacgagacgg cggcggtctc
    13381 gctccacgaa ctcgccgatg ccgttatagc gtcgcgggag ggcgatcccg gcccgctccg
    13441 ggatatgatc cgcccgcccg aggcaaccgt cgtggatctg gcgaaggtcg tcctccgcga
    13501 caccgcggtc gacgccgtct gccacggcgc ggtgcttgcc ggcgtgggga tcgctggaaa
    13561 ggagggagga tttgcgaaag gcgagacggt cgccatcatg accgggcgtg gcgagctcgt
    13621 gggcctcggg aaggcgctcg tcggttcatc ggcgctgaaa cccggggagc ccgggttcgt
    13681 cgtcgccccc accaccatcc tgatgcagcc cggcacctat ccacggggct ggaagacgca
    13741 cgccagggag ccgtagagat attattaata gactcacacg ccaactaatg atgcaccttc
    13801 tgtgctgagg tagtctagac cggtaaggcg cgggcctgga aagcccgtgg ggcgttgagc
    13861 ccctcgggag ttcaaatctc cccctcagcg ttattttata ccgtcaacgc tcgcttcgtt
    13921 atgc
//
LOCUS       DAOSEE010000050        13737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1747_length_13737_cov_255.784461, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000050 DAOSEE010000000
VERSION     DAOSEE010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 13737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13737
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1747_length_13737_cov_255.784461"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PLI76_05330"
     CDS             <1..326
                     /locus_tag="PLI76_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61486.1"
                     /translation="NCTSINANCYGLQVDLAKNVRIENFHLENPAGINGKGASLGGTN
                     GQFGDSSVDIVASGDRVETLVWAKNNQNVEYSGEIRSGSARAFLVEGGGNVRTSGMKV
                     MPAGE"
     gene            complement(426..1565)
                     /locus_tag="PLI76_05335"
     CDS             complement(426..1565)
                     /locus_tag="PLI76_05335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOI61487.1"
                     /translation="MARVAYDISRNLHENGHAVTVYTTNRSIYPSDLPTNRPTDVDGM
                     NVYYFENLRRYIPGATPPVIPYRMPAVAKRETARFDVIHIHDHRTLLTVIASHYARKY
                     GVPYVLQAHGALPDDTGSARMKRLFDVLWAKKVILGAAGVIALNETEAERYRNLGVDE
                     EKIAIVPNGIDLAEYPDLPPRGRFRAAWGIDDATKVVLYLGRLDPTKGIDLLIRSFGE
                     VGQEFGDAVLMLVGGDMGHNEEFRRRVRSLGLDDRVIFTGFVSREDKMAAFTDADVFV
                     TPSFTGFPITFLEACLCGTPIVTTGNGDLLGWVDNTVGFNTAYTPEALAGAIGRLLAD
                     DALRDGFSRQAKEIVRTRYNWQAIVHEIEALYATVANGYREPRGG"
     gene            complement(1616..2698)
                     /locus_tag="PLI76_05340"
     CDS             complement(1616..2698)
                     /locus_tag="PLI76_05340"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOI61488.1"
                     /translation="MTPVRLRAVGDVLLWTRNGKRPFDLVHHELQEKDLLFGNLETVL
                     SESGDPSRKRWVNTTPPEAAEYLEEAGFDILSLANNHTLDMGREGFEKTLDVLESHGI
                     AYIGGARGDGPPQALVVERRGIKLGFLAYTNGMFRSPPGVAVSRLKKKIVIDEIRRLK
                     DRCDHIIVSLHWGIENVYYPSPAQVRLAHDLIDGGATLILGHHSHTIQGLERYKDGLI
                     AYSLGNFQFDTGLFKEKIKSTMILSVDLDRGGIRDYTVIPCFINDDFQPEAAEGRIGE
                     KVERWITECSESVQNGRVTRRWWFEEIGEEYITYNLESYRYRIRQHGLAPLVECGVWL
                     VTPFCLNCYAGVIRRRLRERYTEGYA"
     gene            3400..4152
                     /locus_tag="PLI76_05345"
     CDS             3400..4152
                     /locus_tag="PLI76_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844608.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOI61489.1"
                     /translation="MIDTMRGKAALLGIALMVLCAAVIGNVTAETPEESGDKLIHVSG
                     TGKVTTTPDQAIIVLAVEIENADAKVAQQQNAQKMDAVTNALKNAGIPAKDIRTAGYN
                     IMPVTEIDDKPLATSRVKFYRVTNNLEITLHDVDRAGEIVDIAVANGANRVDRLMLTL
                     SDEKQQQFRSEALTAAVKQARSDADAVVAALGKTVVDVKEVNVGSNYIPMAYDNRYLA
                     ATDMAKGASVPTPVEIGEIDVTATVSIAYIIA"
     gene            4274..4936
                     /locus_tag="PLI76_05350"
     CDS             4274..4936
                     /locus_tag="PLI76_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOI61490.1"
                     /translation="MFTITEVYNNIPCREGLTPDWGFSCLVEEAGLLFDTGAKGDVLL
                     SNMRALGIDPADIRYLVLSHDHWDHTGGVRAILGVNPSMEVFVHDAFTAQTLGMIGEY
                     TKPRIVREWMEIADGIAVTGPLGTDIREQSLAITAPGGYLVITGCAHPHIGRIVDRVS
                     QEGRVWGVVGGFHTVTTGDIGALAGVEYLSASHCTDAIPEIRERYPGTFRPGGVGKVH
                     RV"
     assembly_gap    4946..5045
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5298..6170)
                     /locus_tag="PLI76_05355"
     CDS             complement(5298..6170)
                     /locus_tag="PLI76_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOI61491.1"
                     /translation="MGVIVTFNVTEIFEIPIGVGSITVENLLYFAVIIIVGVAVAKTV
                     STNVRRALVERLPKNEREVLVKLVYYIIVTGALVIALPQLQFDLSGLLVAGGIVGLVI
                     GFASQSVVSNLVSGLFLMFEHPIKIGDNINVAGISGSVEDIRILSTVVKTYDGIFTRI
                     PNEKVFTSNIANYVHNAARRFEYQIGIRYEDDAEVAIRVIKEVIWTHPFALKNPAPSV
                     FVDNFGDDGVNLTAYIWAPTRNWWDVRTSLLWQIKQKLEENGIEMPFPQRTVRFADDL
                     RVTAGDAGKGGAED"
     gene            complement(6155..6859)
                     /locus_tag="PLI76_05360"
     CDS             complement(6155..6859)
                     /locus_tag="PLI76_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF432 domain-containing protein"
                     /protein_id="HOI61492.1"
                     /translation="MFGRYSGTFRHENGDILVETERNGGFLTYRRNCEGRTFERILIS
                     ETGETIINPVEPVNLPGDVTDLFMVEFSPMLIEPGITQTIYLKFPIEVGVFLEAAKDI
                     EVLDIFGLGTQKYTLYGPPTSGAIARWYRSAVYPDIPAVERLREGVMELSIQNMSHEW
                     VEVSCAVFDSVDMKIYYGDFVATTATMKIVTTHMAETDFIDAPLVPGMARSIELYTAR
                     KIPVIKRGYLMEWGLS"
     gene            7373..8794
                     /locus_tag="PLI76_05365"
     CDS             7373..8794
                     /locus_tag="PLI76_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI61493.1"
                     /translation="MGIFDHLRQPALVLDCEGRVVIWNESMERYTGIRAEDIVGEGGY
                     AHGEALFGEKFPTLANRILSRGDAGDDTRYRLSAREDGKVIAGTCISPVSGREVASMA
                     APLYDTAGRQIGAVETIQDTPDARPLRDPPGVPDEQAQILADALSCMIFTLDWDGIYS
                     RFFTTGAAPGSVPEEIVGKTPHALFPAAEADFLVEAARRVIGAGEVVAEMRSFEWRGE
                     QRSFEVTMQPLHDPKGRIVAAAGLSFDVTRDLLRERALQEANQASSLYLDLLGTDIYN
                     TSMVAATVIEMLRERLSGEDAELAQRAKNTVEQGINVIKNVELLNTLNKHRIRLEPVD
                     LDEIIRGQIRRYAGIDIRYEGGSCMVWANPLLEHIVSNLISNSIKFGGMKVRIDISVM
                     ETAEVVTLTVADTGIGIPDPLKPNIFDRFNRDARSVSGSRGLGLYIVKTLTNQYGGRV
                     WAADRVPGRPEAGAAIKVILQRC"
     gene            8951..9391
                     /locus_tag="PLI76_05370"
     CDS             8951..9391
                     /locus_tag="PLI76_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusA-like transcription termination
                     signal-binding factor"
                     /protein_id="HOI61494.1"
                     /translation="MIRTICFKERRYIEELRILTRATALDCIIDERFDRIVYLIKEGD
                     MGLAIGRKGSNIRKMQRVLGKRIEMVEYSPEIESFTRNVFKPAEVAGVKKEDDGRLTV
                     YINKGDLGIAIGKGGCTIEKARILLSRYFDTELAGVLAREDVHA"
     gene            9384..9677
                     /gene="hisE"
                     /locus_tag="PLI76_05375"
     CDS             9384..9677
                     /gene="hisE"
                     /locus_tag="PLI76_05375"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HOI61495.1"
                     /translation="MRDWSILDDLWLVIQDRAEHPSPESYVTSVLTHRKGIDKSLEKV
                     GEEAVEFILAAKNRVPERTVSEAADLLFHLLVALQASGTDVADVLDELAARRK"
     gene            complement(9710..10120)
                     /locus_tag="PLI76_05380"
     CDS             complement(9710..10120)
                     /locus_tag="PLI76_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOI61496.1"
                     /translation="MSVSEMGAAPTVVLDAGGDSTIPIYVGLWEAISISNALNSEMLP
                     RPITHDLVVELFRNFEIVLDALHIDSLEEGVFYAKLLLRQGSRTEVMDCRPSDGIAIA
                     LRYRAPIMIEDTVVESAAVKKDDLPRMVDLKEYL"
     gene            complement(10203..11243)
                     /locus_tag="PLI76_05385"
     CDS             complement(10203..11243)
                     /locus_tag="PLI76_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HOI61497.1"
                     /translation="MKKSQWKWLLVSISFSTLVLAGVLYFTVDETTIDYLSRVNTFYL
                     LLATLLHIVSLGFWALRIQAMSASLGYRVNFRHCLNLVFANMLVAAVTPSQAGGEPVR
                     IHELYRAGVPVGDATAVVVMERILDGIILGALGAFAMLFLGRYWSSLASGFSGISFIM
                     YAAWIAVTLFVLIFVYSVKNPDHLKRALRRLSCWIDRRWHLRRLEQLLGTIDREVDNF
                     NQGLVKFVNHGKSGLVWGLIFTTFFWFSEFIIASLLLVGLGQPPYFIESFVVQIVIAV
                     IMMIPLTPGGSGIAELGATSLYSLIIPSSIVGIFVLLWRLILYYLNILLGLLSSLVIV
                     RREFLARAEKQQ"
     gene            complement(11422..11991)
                     /locus_tag="PLI76_05390"
     CDS             complement(11422..11991)
                     /locus_tag="PLI76_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF357 domain-containing protein"
                     /protein_id="HOI61498.1"
                     /translation="MTLEEQGALYLAALRRTRIAVPEGTLLYRAAEEVLEMAVAYQSD
                     GMAFLRAGDHVNALAGFAYGLGWLDAGSRLGLLLPLAAHPPGGAGVCIPEALAARLDE
                     KTHRYRRMLQSALPAIEVGPDEASPLFCGAEELRSTARCWYTDGVNRLDAGDPAGALA
                     RFSYGYAWLDAGIRAGLFRITGERGLFTV"
     gene            complement(11988..12740)
                     /gene="dph5"
                     /locus_tag="PLI76_05395"
     CDS             complement(11988..12740)
                     /gene="dph5"
                     /locus_tag="PLI76_05395"
                     /EC_number="2.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004164 - diphthine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0017183 - peptidyl-diphthamide biosynthetic
                     process from peptidyl-histidine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine synthase"
                     /protein_id="HOI61499.1"
                     /translation="MLTFVGLGLYDPEDISVKGLECVKSADVVYLESYTSRLMGTDVA
                     GMEAFFGKEVRVLAREDVEQDPRDIIEHALHGHVAFLTGGDPMVSTTHADLRIRAAAA
                     GVATAIVHASSISSAVCGLSGLQNYRFGKSCSVPFPAKGWFPTAPVETIAANLACNLH
                     TLVYLDIQKDRYMRIPEAIAIVEEMAAKRGIEPPALYVGIARAGSACPVVAAGTGADL
                     KETDFGPPLHILVVPGDLHPVEREYLEIFAGL"
     gene            complement(12766..13491)
                     /locus_tag="PLI76_05400"
     CDS             complement(12766..13491)
                     /locus_tag="PLI76_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61500.1"
                     /translation="MYRYEIGIPMIDREFGGLRAASNVLILAPPLTYAEHLAYRMACP
                     RAGEWTIALSTDERAAEVVGAFRKQGAQRNQVGIIDAVTKSSVPTLRDTAKAKFVTSP
                     LDLTSMGIKLSRMVEDMWREGVMADPPGPIPPPIRLCVNSVSTILMYARLEVTFKFLH
                     VITNRMKKFEGIGIYILNSESFDERTIATIKQLMNIVIEVRTEDGRQSVERDFRVIGV
                     RGRTTPWIRYFYDDGTLTIEEWR"
     gene            complement(13505..>13737)
                     /locus_tag="PLI76_05405"
     CDS             complement(13505..>13737)
                     /locus_tag="PLI76_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOI61501.1"
                     /translation="MAFETLAILAGTADLGAVLSSLQLYIFAVVTVLFIPCLATITVL
                     LREVGSRITLAVTIYTVALGLFIGGLMYRILA"
BASE COUNT         2649 a         4244 c         4224 g         2520 t
ORIGIN      
        1 agaactgcac gagcatcaac gcgaactgtt acggcctcca ggtcgacctc gcgaagaacg
       61 tccggatcga aaacttccac ctggagaacc cggccgggat caacggcaaa ggtgcaagcc
      121 tcggcggcac gaacggccag ttcggcgatt cgtcggtcga tatcgtcgcc tccggcgacc
      181 gggtcgagac gctggtctgg gcaaagaaca accagaacgt cgagtactcg ggcgagatcc
      241 gatccggctc cgccagggcg ttcctggtcg agggcggcgg gaacgtccgg acctcgggca
      301 tgaaagttat gcctgccggc gagtaaaccg atacctcgtc ctcaaccttt ttttaaagaa
      361 cgcgcctgcg ggctcccgta cctcttttgt gcttcccggg cggccggatc cccgccggcg
      421 aacgcttatc cgccccgcgg ctccctgtag ccgtttgcta cggtcgcata cagggcctcg
      481 atctcatgga cgatcgcctg ccagttgtag cgcgtccgga cgatctcctt cgcctgcctg
      541 ctaaacccgt ctcgcagggc gtcgtcagcg agcaggcgcc cgatggcgcc ggcaagcgcc
      601 tccggtgtat atgcggtgtt gaacccgacg gtgttgtcga cccatccgag gaggtccccg
      661 ttccccgtcg tcacgatggg cgttccgcag aggcatgcct cgaggaacgt gatgggaaag
      721 ccggtgaagc tcggcgtcac gaagacgtcg gcgtcggtga acgcggccat cttgtcctcc
      781 ctgctcacga accccgtgaa gatcacccgg tcgtcaaggc caagcgaccg gacccgccgc
      841 ctgaactcct cgttgtggcc catatctccg cctaccagca tgagaacggc gtcgccgaac
      901 tcctgcccga cctctccaaa cgagcggatc aggagatcga tcccttttgt cgggtcgagt
      961 cgcccgaggt agagcacgac cttcgtggcg tcatcgatac cccatgctgc gcggaacctg
     1021 ccgcggggag ggaggtcggg gtattcagca agatcgatgc cgttcgggac gatggcgatc
     1081 ttctcctcat cgacgccgag gttgcggtag cgctctgcct ccgtctcgtt gagcgcgatc
     1141 actcctgcgg cgccgaggat caccttcttt gcccagagaa cgtcgaacaa ccgtttcatc
     1201 cgcgcagaac cggtgtcgtc ggggagcgcc ccgtgcgcct ggagcacata cggcacgcca
     1261 tactttcgcg cgtagtgcga ggcgatgacg gtgaggaggg tgcggtggtc gtggatgtgg
     1321 ataacatcga atcgtgccgt ctctctcttt gcgaccgccg gcatgcggta gggtatcacc
     1381 ggcggtgtcg cgccggggat atacctgcgc aggttctcga agtagtagac gttcataccg
     1441 tcgacatcgg tcgggcggtt cgtcggcagg tcgcttgggt agatgctccg gttcgtcgtg
     1501 tagaccgtga cggcgtgccc gttctcgtga aggttccgcg atatgtcgta tgcgacccgc
     1561 gccactcccc ccgactccca gaccggttta aaaaacggtg taacctgcaa tatattcacg
     1621 catatccctc tgtatatcgt tctctcaacc gtctcctgat cacgcctgca taacagttca
     1681 ggcagaacgg ggtcaccaac cagaccccgc actcgaccag gggggcgagg ccgtgctgcc
     1741 tgatgcggta gcggtaactc tccaggttat aggtgatgta ctcctcgccg atctcctcaa
     1801 accaccacct ccgggtgacc ctgccgttct ggacgctctc cgaacactcc gtgatccagc
     1861 gctctacctt ctcccctatc cgcccctccg ccgcctccgg ctggaaatcg tcgttgatga
     1921 agcaggggat gaccgtatag tcccggatac cgccccggtc aaggtctacc gagaggatca
     1981 tggtgctctt gatcttctct ttgaagagtc cggtatcgaa ctggaagttc ccgagggagt
     2041 aggcgatcag gccgtcctta taccgttcca gcccctggat ggtgtgcgag tggtgcccga
     2101 gtatcagggt tgctccgccg tcgatgaggt cgtgggcaag cctgacctgg gccggagagg
     2161 ggtagtagac gttctcgatc ccccagtgca gggagacgat gatgtggtcg cagcggtcct
     2221 tcagcctcct gatctcgtcg atgacgatct ttttcttcag cctggatacg gcgacgcccg
     2281 gcggcgaccg gaacatgccg ttcgtatacg ccagaaatcc gagtttgatc cccctcctct
     2341 cgacgaccag ggcctgcggc ggcccgtcgc cccgtgcgcc gccgatgtag gcgataccgt
     2401 ggctctcaag cacgtcgagg gtcttttcaa acccctcccg ccccatatcg agcgtatggt
     2461 tgttcgcgag gctcagtata tcgaacccgg cctcttcgag gtattcggcc gcctccggcg
     2521 gggttgtgtt gacccagcgc ttccggctcg gatcgccgct ctcggagagg accgtctcca
     2581 ggttcccaaa gagcaggtcc ttctcctgga gctcatggtg taccaggtca aacggtcttt
     2641 tcccgttcct ggtccagagc agcacatccc cgacagcccg cagccgcacc ggcgtcatac
     2701 ctctctcccg gaaagggagg ggccgcgcct gaaacccgct ttcatcgcgg ttctctccgg
     2761 gcagggccgc cgtgtgcgga tcccccggaa cactcccctc cccctcgaag catcgagctc
     2821 cagacaggca ttgcagcgat atataccttt gcctcgattg ccgccccgga taggccgctc
     2881 ccggcgcttc cggcagccct gtgagcctcc ggactccggg tggagcacgg agcggttcca
     2941 gcactgcaac acccggggcg ccgaagcatt aacaggtcat acgccgacca tgtgatggca
     3001 gatgatgaga gttaccccca ctgatccgtg tgatgaagca ggggtctgat gccgttctgt
     3061 atttatcctg agagccggtc tcccgtaccg ggcatgcggt acccgggcac cggaccattc
     3121 cccgccttga ggctctcttc ttccggggca cccgcacggg cgtgcgggtg gctgtaaccg
     3181 tcgagttttg caggatctgc cgggacggga gggcatgcgg tatcctgcgg gtatgagccg
     3241 gccccgctct catccctgcg ccttccgggc ttgggtcttc ccgcttctcc atcctctcac
     3301 ctccgggaga taaaaacgga catttccggg atcggcgctc ttaaaactac gaaaattccc
     3361 gtagttaaag caagatattt attttcgtca aatcaagaga tgatcgatac catgcgagga
     3421 aaggcagcac tcctggggat cgccctgatg gtcctctgcg ccgcggtgat cgggaacgtc
     3481 accgcagaga cgccggagga atccggggac aaactgatcc acgtatccgg caccggcaaa
     3541 gtaacgacca cgcccgacca ggccatcatc gtgctcgcgg tcgagatcga gaacgccgat
     3601 gcgaaagtcg cgcaacagca gaacgcccag aagatggatg ccgtcacgaa cgccctgaag
     3661 aacgccggca tcccggcaaa ggatatcagg accgccggct acaacatcat gccggtgacc
     3721 gagatagacg acaaacccct ggcgacctcc cgggttaaat tctatcgggt gaccaacaac
     3781 ctcgagatca ccttacacga cgtcgatcgt gcaggcgaga tcgtcgatat cgcggtcgcg
     3841 aacggggcaa accgggttga ccggctcatg ctcaccttga gcgatgaaaa acagcagcag
     3901 ttccggtcgg aggccctgac cgccgccgtg aaacaggcgc gaagcgacgc ggatgcggtc
     3961 gtcgccgccc tcggcaagac cgtcgtcgat gtcaaggagg tcaatgtcgg gagcaactac
     4021 atccccatgg cctacgataa ccgctacctg gccgctactg atatggcaaa gggcgccagc
     4081 gtcccgaccc cggtcgagat cggcgagatc gacgtgaccg cgacggtctc catcgcctac
     4141 atcatagcgt aaccctcttt ttcgtctgca ggcccggggg tgctccgaac tcccgggccc
     4201 gatacccccc gggtctgccg catcgggtag cataatcgtt tgggaagaga gaccgggaga
     4261 tggagttttc atcatgttca ccatcaccga ggtctacaac aacatcccct gccgggaggg
     4321 tctcacgccg gactgggggt tctcctgcct ggtcgaggag gcggggctgc tctttgacac
     4381 cggggcgaaa ggcgacgtac tgctatccaa catgcgggcg ctcggcatcg accctgcgga
     4441 tatcaggtac ctcgtcctct cccacgacca ctgggatcat accggagggg ttcgcgcgat
     4501 cctcggcgta aacccctcga tggaggtctt cgttcacgac gccttcaccg cgcagacgct
     4561 cgggatgatc ggggagtaca caaaaccccg gatcgtccgg gagtggatgg agatcgcgga
     4621 cggtatcgcc gtcaccggcc cgctcgggac cgatatacgg gagcagtcgc ttgcaatcac
     4681 cgcccccggc ggctatctgg tcatcaccgg gtgcgcgcat ccccacatcg gccggatcgt
     4741 cgaccgggtc tctcaggagg gcagggtatg gggggtcgtc ggcggattcc ataccgtcac
     4801 caccggggat atcggcgcgc tcgccggtgt ggagtatctc tcggcatcgc actgcaccga
     4861 tgcgatcccc gagatccggg agaggtatcc ggggaccttc cggcccggcg gggtggggaa
     4921 ggtgcaccgg gtgtgagaga ggggtnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnngggga ccttccggcc cggcggggtg gggaaggtgc accgggtgtg agagaggggt
     5101 gcatggcacc ctgcagagaa tttacccatt tacggagtat gggggcacat aacgcccctt
     5161 tgcgctcttt tgcgtgaggc cgtttcaccg ctcataccct cgagttcgcg cgaagcccga
     5221 tcccgggtac ctgaagcgcc ccttcgcagc ctttatacca cgggggccgc attccccgca
     5281 gttccctgaa cccggggtca gtcttctgcc ccgcctttcc cggcatcgcc ggccgtcacc
     5341 cggaggtcat ccgcaaaccg gacggtcctc tgcgggaagg gcatctcgat gccgttctcc
     5401 tcgagtttct gcttgatctg ccagaggagg cttgtcctga cgtcccacca gttccgggtc
     5461 ggcgcccaga tgtaggccgt caggttgacg ccgtcgtcgc cgaagttatc gacgaagacc
     5521 gacggtgccg ggttcttgag cgcgaacggg tgggtccaga tgacttcttt gatgacccgt
     5581 atcgccacct cggcgtcgtc ctcgtagcgg atgccgatct ggtactcgaa cctgcgggcg
     5641 gcgttgtgaa cgtagttggc gatgttcgac gtgaagacct tctcgttcgg gatccgggtg
     5701 aatatgccgt cgtacgtctt gacgaccgtc gagaggattc gtatatcctc gacgctgccg
     5761 ctgatgcccg cgacgttgat gttgtcgccg atcttgatgg ggtgctcgaa catgaggaag
     5821 aggcccgaga ccaggttcga gacgacgctc tggctcgcaa acccgatgac gagaccgacg
     5881 atgcccccgg ccaccaggag gccggagagg tcgaactgga gttgcgggag ggcgatgacg
     5941 agcgccccgg tgacgatgat gtagtagacc agtttcacga gtacttcgcg ctcgttcttt
     6001 gggagccgct ctacgagggc tctccggacg tttgtcgaga ccgtctttgc gacggcgacg
     6061 ccgactatga tgatgacggc gaagtacagg aggttctcga cggtgatcga cccgacgccg
     6121 atggggatct cgaagatctc ggtcacgttg aacgtcacga taacccccat tccatgagat
     6181 accctcgttt gatcaccggt atcttccgtg ccgtgtagag ctcgatggat cgcgccatac
     6241 cgggcacgag cggggcgtcg atgaagtccg tctccgccat atgggtggtg acgatcttca
     6301 tcgtggccgt ggtcgccacg aagtcgccgt agtagatctt catatcgacg ctgtcaaaga
     6361 cggcgcacga gacctcgacc cactcgtggg acatattctg tatggagagc tccatcaccc
     6421 cctcgcggag acgctcgaca gcggggatat cggggtagac cgcgctccgg taccagcggg
     6481 cgatggcgcc gctggtcggc gggccgtaga gcgtgtactt ctgggtgccg aggccgaata
     6541 tgtcgagcac ctcgatatct tttgccgcct caaggaagac gccgacctcg atggggaact
     6601 tcaggtagat cgtctgcgtg ataccgggct cgatgagcat cggagagaac tctaccataa
     6661 agagatccgt gacatccccg ggaaggttga ccggttcgac cgggttgatg atggtctcgc
     6721 ctgtctccga gatcagtatc cgctcgaacg tccgtccttc gcagttccgc cggtaggtca
     6781 aaaaaccgcc gttccgctcg gtttctacca gaatatcgcc attttcgtgg cggaaagtgc
     6841 cagagtaacg cccgaacatc actctatgat ctgcaaataa tcaaatatta aggtacgcga
     6901 ccggccgcga cctgccgacg ttcgcgaccc tgcacccgat cgttgatgac ggagcatatc
     6961 ggtctttcgg tcgtgccgtt ccatgaaacc aattcattgt atcaaccccg gccattatgc
     7021 ccgggagcga tccggcaagg cataaatatc aggccttccg ggaagcacgg tcttcggggc
     7081 atcattagac atccttttaa gcggtcatcc cagacatttt ttaacgtgca tctgttccgg
     7141 tccccgggca agcaccccta tccttcgcca ccatccgggg ctgctgtcgc gggagaacgg
     7201 ggtaccggga ccgggagcac agatatgggg gcgccgtacc tgccgctgcg aagaaccctc
     7261 ccggaggaga ggactccttt gcggttgtgg gaggcacgat cggccccggg tgatacccgt
     7321 gaaggtaaac cacgagaata ccagagaatc cgtgaattgc agccagatac ccatggggat
     7381 cttcgatcac ctccgacaac cggcactggt tctcgactgc gaaggaaggg tggtcatctg
     7441 gaacgagagc atggaacgat acacggggat ccgggccgaa gatatcgtcg gggaaggagg
     7501 atatgcgcac ggggaggcgt tgttcggcga gaagttccct acgcttgcaa accgcatcct
     7561 ctcccggggc gacgccggag acgacacccg ctaccgcctc tctgcccgtg aagatggaaa
     7621 ggtgatcgcc ggaacatgca tcagtccggt atccggcagg gaggtggcca gcatggccgc
     7681 ccccctctac gacaccgccg gcagacagat cggcgcggtg gagacgatcc aggatacgcc
     7741 ggacgcccga cccctccgcg atccccccgg ggtgccggat gagcaggcgc agatcctcgc
     7801 ggatgccctc tcctgcatga tcttcacgct tgattgggac ggcatctact cccggttctt
     7861 cacgaccggc gccgcccccg gatcggtccc ggaggagatc gtcgggaaga ccccccacgc
     7921 cctcttcccg gcggcggagg cggacttcct ggtagaggcc gcacgccggg tcatcggggc
     7981 gggggaggtt gtggcggaga tgcggtcgtt tgagtggcgt ggcgaacaac ggtcgtttga
     8041 ggtcaccatg caacccctgc atgacccaaa aggaaggatc gtggctgccg ccggtctcag
     8101 ctttgatgtc acccgggacc tcctccgcga acgggccctc caggaggcca accaggcctc
     8161 cagcctctac ctcgacctgc tcggcaccga catctacaac accagcatgg ttgcggcaac
     8221 ggtcatcgag atgctccggg aacgactctc cggcgaagat gcggagctcg cccagagggc
     8281 caagaacacg gtcgagcagg ggatcaacgt catcaagaac gtcgaactct tgaacacgct
     8341 caacaaacac cgcatccgcc ttgaaccggt cgatctcgac gagatcatac gcggccagat
     8401 acgacgctat gccggcatcg acatcaggta cgaggggggg agctgcatgg tctgggcaaa
     8461 ccccctcctc gagcatatcg tatccaacct gatcagcaac agcatcaagt tcggcggcat
     8521 gaaggtgcgg atcgacatat ccgtcatgga gaccgcggag gtggtgaccc tcaccgtcgc
     8581 cgacaccggg atcggcatac cggacccctt aaagcccaat atattcgacc ggttcaaccg
     8641 tgacgcgagg agcgtatccg ggagcagggg tctggggctt tacatcgtca agaccctcac
     8701 gaatcagtac ggcgggcgcg tctgggcggc ggaccgggtg cccggcagac ccgaggcggg
     8761 agcggccatc aaggtgatcc tgcagaggtg ttagcgcccc gcgccgcccg aaaacctgca
     8821 gatcccgtgc catcgcttcg cggatcccat gacagcccgg atcgcctgcc ggcaggcgat
     8881 cccggtactc agatatataa cctcctgatg actatgctat accgcatcat tcgcatcgga
     8941 gacttctcca atgatacgaa ccatatgttt taaagaacga cgatatatcg aagagctgcg
     9001 aatcctcacc cgggcgacgg cgctggactg catcatcgac gagcgcttcg atcgcatagt
     9061 atacctcata aaagaaggag atatgggcct tgccatcggc cggaaaggga gcaacatccg
     9121 gaagatgcag cgagtcctcg ggaagcgcat cgagatggtc gaatactctc ccgagatcga
     9181 atcattcacg agaaacgtgt tcaaaccggc ggaggtcgcc ggcgtgaaga aggaagacga
     9241 cgggagactc accgtataca tcaacaaggg cgatctcggg atcgccatag ggaaaggggg
     9301 atgcaccatc gagaaagcac gcatcctcct ctcccggtac ttcgacaccg aacttgccgg
     9361 ggtgctggca cgggaggacg tccatgcgtg actggagcat cctcgacgat ctctggctgg
     9421 tcatccagga ccgcgccgag cacccgtcgc ccgagagcta cgtcacctcc gtcctgaccc
     9481 accggaaagg gatcgacaag tccctggaga aggtcggcga agaggcggtc gagtttatcc
     9541 tcgccgcaaa gaaccgggtt ccggaaagaa cggtatctga ggcggccgat ctcctctttc
     9601 atctcctcgt ggcgctccag gcatcgggca ccgacgtggc ggacgtgctc gacgagctcg
     9661 ccgcacgccg gaagtaacgg caagcttccc tctcccccgg ggcgatcgct cagaggtact
     9721 ccttcaggtc caccatcctc gggagatcgt ccttcttcac cgccgcggac tctacgactg
     9781 tatcctcgat catgatcggc gcccgataac gcagagcaat ggctataccg tcgcttggcc
     9841 ggcagtccat gacctcggtg cgcgagccct gccggaggag aagtttggcg taaaaaaccc
     9901 cctcctccag agaatcgatg tgcagggcat cgagcactat ctcaaagttc cggaagagtt
     9961 cgacgaccag gtcgtgggtg atgggccggg ggagcatctc gctgttgagc gcgttgctga
    10021 tcgagatcgc ctcccagagc ccgacgtata tcggtatcgt gctgtcgccc ccggcatcca
    10081 ggacgacagt cggcgctgcc cccatctcgc tcaccgacat gaacacgccc tgcaccctgc
    10141 aactttgagc agtcatgtga tcatctgaca tgagtcttat gatgtttaaa ccttggggtc
    10201 gatcactgct gcttctcggc acgggcgagg aactctcgcc gtacgatgac gagactcgag
    10261 agcaggccga gaaggatatt gaggtagtag aggatcaacc gccagaggag gacgaagatg
    10321 cccacgatcg acgacgggat gatgaggctg taaagcgacg tggcgccgag ctccgcaatg
    10381 cccgagcccc ccggcgtgag cgggatcatc atgatgacgg cgatgacgat ctggacgacg
    10441 aacgactcga tgaagtacgg cggctgcccg aggcccacga ggaggagcga tgcgatgatg
    10501 aactccgaga accagaagaa cgtcgtgaag atcagccccc acacgaggcc gcttttgccg
    10561 tggttcacga acttgacgag tccctggttg aagttgtcca cttcccggtc gatcgtgccc
    10621 aggagctgtt cgagccgccg caggtgccag cgccggtcta tccagcacga gagccgccgg
    10681 agagcccgtt tgaggtggtc ggggttcttc accgagtaga cgaagatcag gacgaagagg
    10741 gtgacggcta tccaggcagc gtacatgatg aaacttatgc cgctgaaccc cgaggcaagg
    10801 ctgctccagt acctgccgag gaagagcatg gcaaaagccc cgagcgcccc gaggatgatc
    10861 ccgtcgagta tccgctccat gacgacgacg gcggtggcgt ccccgaccgg aacgcccgcc
    10921 cggtagagtt catggatccg gaccggttcg ccgccggcct gtgacggggt gaccgccgca
    10981 acgagcatgt tcgcgaagac caggttcagg cagtgcctga agttcacccg gtacccaagc
    11041 gacgccgaca tcgcctgtat ccggagcgcc cagaacccga gcgagacgat gtggaggagg
    11101 gtggcaagga gcaggtagaa cgtgtttacg cggctgaggt agtcgatcgt cgtctcgtcg
    11161 acggtgaagt agaggacgcc cgcgagaacg agggtgctga agctgatcga gacgagcagc
    11221 cacttccact gagacttctt catcgctctg tactccccgg gtcggtgtcc ggcgacaccc
    11281 acggttcgtt gatgatttcg ggcagattca ggagagaatt acccggaatt ggactaataa
    11341 ggtatcgtgt ggctactatt taacgatgat tttcggtcgc gattcggcga tatctccggt
    11401 tcgctgcggg aggcgattct ctcagaccgt aaagaggccg cgctcgccgg ttatccggaa
    11461 gagtcctgcc cggatgccgg catcgagcca ggcatacccg tagctgaacc gggcgagggc
    11521 gccggcaggg tcgccggcat cgaggcgatt tacaccgtcc gtgtaccagc aacgcgccgt
    11581 cgaacggagt tcctccgcgc cgcaaaagag cgggcttgcc tcgtccggcc ccacctcgat
    11641 cgccgggagg gcggattgga gcatccgacg gtagcggtgg gtcttctcgt ccaggcgggc
    11701 cgcgagcgct tccggtatgc acacgcccgc cccgcccggc gggtgggcgg cgagcggaag
    11761 aagcagtccg agccgcgagc ctgcgtccag ccagccgagc ccgtatgcaa acccggcaag
    11821 agcgtttacg tggtcgcccg cccggagaaa cgccatgccg tcgctctggt acgcaacggc
    11881 catctcgagc acctcctccg ccgcccggta gaggagcgtc ccctccggga cggctatccg
    11941 ggtccggcgg agagccgcga ggtagagggc accctgttct tcaagggtca taggccggcg
    12001 aagatctcca ggtactcccg ctcaacgggg tggaggtctc cgggcacgac gaggatgtgg
    12061 agcgggggtc caaagtccgt ctctttgagg tctgcgccgg tacccgccgc gaccaccggg
    12121 cacgccgacc ccgcccgggc gatgcccacg tagagcgcgg gcggctcgat gccgcgcttt
    12181 gccgccatct cctcgacgat cgcgatcgct tccgggatgc gcatgtagcg gtccttctgg
    12241 atatcgaggt agacgagcgt gtgcaggttg caggcaaggt tggccgcgat cgtctcgacg
    12301 ggcgccgtcg ggaaccaccc cttcgcgggg aacggcaccg agcaggattt tccgaaccgg
    12361 tagttctgga gccccgagag gccgcagacg gcgctcgata tggacgaggc gtggacgatc
    12421 gcggtcgcaa cgccggcggc ggccgctcgt atccggaggt cggcgtgcgt cgtcgatacc
    12481 atggggtccc ccccggtcag gaacgccaca tggccgtgga gggcgtgctc gatgatatcg
    12541 cgggggtcct gctcgacgtc ctcgcgcgcg agcacccgga cctctttgcc gaagaacgct
    12601 tccatcccgg caacgtccgt ccccatcagc cgcgacgtat acgactccag gtagacgaca
    12661 tcggcacttt taacgcactc gagccctttg accgatatat cttccgggtc gtagaggccg
    12721 aggcccacga acgtcagcat gagaggcgtg ctccgggata gggtatcaac gccattcctc
    12781 aatcgtcagc gttccgtcgt cgtagaagta acggatccag ggggtcgtcc tgccgcggac
    12841 gcctatgacg cggaagtccc gctccaccga ctgccgcccg tcttccgtcc tcacctcgat
    12901 gacgatgttc atcagctgtt ttatcgtggc tatcgtcctc tcgtcgaacg actcgctgtt
    12961 gaggatgtag atgccgatcc cttcaaactt cttcatccgg ttcgtgatga cgtggaggaa
    13021 cttgaaggtc acctcaaggc gcgcgtacat gagaatggtc gagaccgagt tgacgcagag
    13081 ccggatcggc ggggggatcg gccccggggg atcggccatc accccttctc tccacatatc
    13141 ctcgaccatc cgggagagtt tgatccccat gctggtcagg tcgagagggc tcgtgacaaa
    13201 cttcgccttt gcggtgtcgc gcagggtcgg caccgaactc ttcgtgaccg catcgatgat
    13261 ccctacctgg ttcctctgcg cgccctgttt ccggaacgca ccgacgactt cggctgcacg
    13321 ctcgtccgtc gagagggcga tcgtccattc ccccgcccgt gggcaggcca tccggtaggc
    13381 aaggtgctcg gcgtaggtca ggggcggtgc caggatgagg acgttcgagg cggcccgcag
    13441 gcccccgaac tccctgtcga tcatcggtat gccgatctcg taacggtaca tgtcagcaca
    13501 tcacttatgc taagatacgg tacataaggc ctccgataaa gaggccgagg gcgacggtgt
    13561 agatcgtgac cgcaagcgtg atccgtgagc cgacttcccg caggaggacc gtgatggtcg
    13621 caagacaggg gatgaagagg acggtcacga ccgcgaagat gtagagctgg agcgacgaaa
    13681 gcaccgcccc gaggtcggcg gtccccgcga ggatggcaag ggtctcaaac gccatct
//
LOCUS       DAOSEE010000051        13735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1749_length_13735_cov_199.723173, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000051 DAOSEE010000000
VERSION     DAOSEE010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 13735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13735
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1749_length_13735_cov_199.723173"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="PLI76_05410"
     CDS             complement(<1..941)
                     /locus_tag="PLI76_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOI61502.1"
                     /translation="MAENFNVRLNEAKYYTPEASCREQSWIGDYNRAYQAFLADPDGF
                     WDSAARELEWFQPWDQVREWEYPHARWFVNAKLNITHNCLDRHAHSQRRNKVALMWRG
                     ETEDEERILTYRQLLQEVCRFANGLSRLGVRKGDRVCIYMPVVPEQIVAMLACARIGA
                     IHTVVFGGFGVSALNQRIRGTAAKVVITADFTYRRGKAIPLKNIVEEAVVKAPSVERI
                     VILRRDAEKPVEIHPEMEVDYHDLMNGVGRECPPEPMDAEDPLFILYTSGTTGSPKGI
                     VHACGGYMVGTYYTTKHIFDVKDSDIYWCTADPGWITG"
     gene            complement(1258..2259)
                     /locus_tag="PLI76_05415"
     CDS             complement(1258..2259)
                     /locus_tag="PLI76_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61503.1"
                     /translation="MNVPDVRYTVRNPTAEPVWVRLVSEYPGYSAPTVSVSEIGAGSP
                     ATFDHFVVLDREEIQEIRAPARVAVHYRIEYWDGGNWTVHDEQTDPVTFYPMDQMVWA
                     TEDKDGVITIYHGLIAIFVTPQSPGVAALAAKAKERATGEFDRRYVDYGMERTLPGYA
                     LPNQRTTYADTKNRTALQVKAIYNALKYDYNLSYVDALVAFGMGDSQRVSTPDESLAT
                     GSANCIDGAVLFASAIERLGMQPYIVVVPGHAYVAWKTDKAGTEVDALETTWVSSCDF
                     EKAYDAGTKRYKEDAKSGRMELYQSLFDRRLSRNEYDSIYVLVEIRWLRSQGILPMK"
     gene            2691..4292
                     /locus_tag="PLI76_05420"
     CDS             2691..4292
                     /locus_tag="PLI76_05420"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOI61504.1"
                     /translation="MKIGMTRICHIAGFFLLVIAISATVQSVQPADAVQADTSNPALQ
                     AVPGADNFPIVQITNSTRDQVLPRISGNRILWMETDRINTRNLYLYDIANRSAEMIIE
                     HFSPRTSPEISGNWIGWVEDQSDSSGKIFPCIRVYDIRSGTITQITQYPAMPYQTSES
                     QDITWERESTLAISGDIIVWHDKRDGNMDIYYRNLSSGVENPIARSAADETCPSISGD
                     RIIWAEQHGTKGYTIHLYNLTSNEHDRIIDLPAMRRNQVISGDYIVWSEWREGSYDVC
                     CYDIASGNTTWITDEPGHDLWPAVSGDAIVWSVYGRGSDDNGISAYDLSTETGTEILA
                     DLPHPVWPDIDGGKVVWSDNRTGNYDVYLCSLENRSDAAPGLYTVHLNSIPSGADISV
                     NNEPYGRTPATLSFDRTGICPIGLKKNGFIPYTTTLDISASLTFVVNLERELVGPVSG
                     PRPILMSITVDSVPRGANVSVDGEVYGTTLFGMDRLPSGDHEVELTLEGYQSNRTTVN
                     SSEPINITLIPGTDADRPLLMERYA"
     gene            4292..5395
                     /locus_tag="PLI76_05425"
     CDS             4292..5395
                     /locus_tag="PLI76_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61505.1"
                     /translation="MFSYLSKRQASTAIILTLLVFMLHYGFSLLFPGYLYLEEASDLI
                     AIAAQSGAIGFQLAGARYLVARLKRTFKDEFPAVPGRGGGDCPYMAARSVLCNRAHYI
                     AFIAAFLSPFIVITLVSLQPDSAPRHLLSLASRSIPGLTFDVFNYGTAYANYWLLGTI
                     AWILLGVVVALNGMEGAGCSVYDAVDIYSPDRIGGLSPVRRYLMSALSIFLISIALLV
                     MGYINLLESFIGSSQGLFHFFFDVVAEICVFSVLSLSGVALTVVGLNRLSAICLKKIE
                     DRVNAVNERCRTFQENLFSLSPDELERRGDEVGSLKLVVEALSDERERLLQRYSDCSG
                     FNARMALRIFVAYIPPLVTIAFQVSQLLPRLRV"
     gene            complement(5463..5762)
                     /locus_tag="PLI76_05430"
     CDS             complement(5463..5762)
                     /locus_tag="PLI76_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61506.1"
                     /translation="MEHTASGADRSPGAVRKGQIDLIAEIAAFADEYGDILARYHRYT
                     MDDLCRIEGECRRLQDEARRRETWGIADELAGLEYLIDRAKAMRAARMAEEDSRG"
     gene            complement(5805..6191)
                     /locus_tag="PLI76_05435"
     CDS             complement(5805..6191)
                     /locus_tag="PLI76_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61507.1"
                     /translation="MIDTAWQDLAITGITILFAVMLLPQLRDVVSRGVVLNCFSAFFT
                     SVLSYTLAMVFATLGLWISVFGQALVASVWLLLAYFSLRNVQASMYPDEALTSVARDF
                     FTVWLQGVAFVVSGGVTGFFSRLRRG"
     gene            complement(6323..8479)
                     /locus_tag="PLI76_05440"
     CDS             complement(6323..8479)
                     /locus_tag="PLI76_05440"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOI61508.1"
                     /translation="MTSRLLFYRTRIIPALALFCIVTLAISSAGCTATAADPIRIGVL
                     LPADGPVALHSLEGLAWAADAMNRRGGRTIELVCRDTSTGNISAYAEELAGRNDIAIV
                     IGPAMSAELIDIAPLVTGRGKLLISPSVTMELATGELNDNDLLWRTCLADGRQVETIF
                     LRLRGDGVRNVSLVTGATPYGETFARLAPEAAAKNGIRITGAASLSASDDLSEIARQI
                     VKEEPDIVVAAVYPEEAIGLVNVLSPDRLFFSDACRSSYLIEHLGERAEGIRGTSLAP
                     DPSTGYAIAYEEIFGEAPPAFAAQTYDAFLLALYTAARQEAAPGESLAASIRWVVTGD
                     GTVKGWDPEEAGEAMALLRAGAHPAVTGASGPLRFGEHSSRGPLAGYYSIWAIEGGAF
                     SEGSPVPSAAIDPYIPDLSFAGSAGDRPVVWMIYPLEKGDRSFADSAYRGLFRAQESF
                     SFTKREFTYADLALLDAVFSLQNFTEKPDLVITEGFEFTGAARAWAAANPDIRFVTLE
                     QEDYGLPNACDVVMVPYGASYLAGVLAANITRTGHIGAIAGAPVAVIEPFVRGFTGGA
                     AAYDPSVNVTVLYVGESFEGFGMPERAGDLARDLRADGVDVILMAAGASGTGVVDAAR
                     ETGDIFLIGVDTDQSYVAPNLVVASVVKGIDTIVYHAVANEIAGRFAPGQEVWTLENG
                     GTGLVLSSRFAEHTWVDTGWRDRAIAAEKEYLKAVA"
     gene            complement(8604..10271)
                     /locus_tag="PLI76_05445"
     CDS             complement(8604..10271)
                     /locus_tag="PLI76_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HOI61509.1"
                     /translation="MTSGSPIDDKSPWLQVIAGFLVLTIIVLIPIGLSWYLHPAPVHV
                     RILAVNDFHGQLTAGKSLSGEPVGSAPVLASYLNAAAGRPATFIALPGDIVGASPPES
                     GLLLDEPTILFWNGLAGDCPQIATFGNHEFDRGTDELMRMVDGGNGATTITHLVDPYP
                     GAAMEYVSSNVVWRVNDTPIAAPYTIRDAGGVGIAFIGATTIETPSIQKPANIEDIVF
                     KDEAESINRYIPEIQEKGVHAIIVLLHEGGSQDAYDGPTREGGNVTGRITGIVAGLDP
                     DVDLVLSAHTHEFTNACLENAGGRSVLVTQAYSYGKAFADIELVIDPATGDVTHRTAE
                     IVPAYANRLSGTDPGAAALLERARDAVRPLTERVVTSASASITGQATGAGESPLGNLV
                     ADGQRAAMQADVAFITTGSLRADIPQGNVTWGDLYAVQPFASTVLSMTLTGEQIRDVL
                     EQQWQTPLPPHNLAVSGLSYTFDDGKPAGEKITEIRVGGAPLEPGANYTAAMVDFLAT
                     GGDGYTVFTKGVNPAYGPLDIDALVTYMGALPEPVGVKAEARIAKAA"
     gene            complement(10524..10826)
                     /locus_tag="PLI76_05450"
     CDS             complement(10524..10826)
                     /locus_tag="PLI76_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61510.1"
                     /translation="MDRTTSRPARCATGSPKGDRLALLEEIAAFVREHADILARYHIH
                     TMADLNRIEQECWRLHDEACSRGACGTAGELAELEYLIGRAKEMKAKRMEGERGPG"
     gene            complement(10954..12288)
                     /locus_tag="PLI76_05455"
     CDS             complement(10954..12288)
                     /locus_tag="PLI76_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61511.1"
                     /translation="MTGQNVLIFVIVALIAVVAGIMIAFAPGETVPGEKASPLDAMSA
                     LATEVAGQIDGDAMAALKPGDESTPAFAAIHDRLNAFRSQHPDIIYIYTLQRTGNAVE
                     YVVDADYGNAGARAIGGGHQVVDPKTPLFAGFEKPSAEVYSAIYGYAPVKNGGMVCLE
                     PEGPIAQERLEALAVEVAGQIDGDAMAALKPGDEETPAYVAIYDRLNAFRAEHPEIAY
                     AYTMCKAGNTVEYVVDADYGSDDGVAIGDGYTPTAIDGVLLRGFEKPSAEIYSLICGY
                     APVKSTSGAGVGMVAVEVGNPITEERLKALAVEVAGDIDGTAMAALRPGDENTPAFTA
                     IRDDLNAFRDATPGVVYVYTMRKTGNATEYVVDADYGNGDAPAIGYTYVPTEEDAAFL
                     AGFEGPSAEPGFYVDDWGNETATVLTGYAPIRDAVGTIVGLVGVDMGIVHES"
     gene            complement(12657..13496)
                     /locus_tag="PLI76_05460"
     CDS             complement(12657..13496)
                     /locus_tag="PLI76_05460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOI61512.1"
                     /translation="MVDPDYYKGKVCIVTGANSGIGYAISEELLKRGAIVYMAGRSRE
                     KVAAAAGQLSAYGADRVRPLIMDVTKQEQVQKGIENTAAEAGRLDLLFNNAGVGGTIP
                     FEKATLDDWKTIIDTNIWSVIYGVHAAVPIMLKQGFGHVVNTSSIAGIAPPPFQALYS
                     LTKYGVTGLTECLRYEYADRGLYFSTICPANIATPIFNKSIDGQAHGELRIPDDAYPV
                     DKAASLILDRVAEHKGIIVVPEEPYTDLWKGYVLGNPEVEERLLQMARERREAFAKGG
                     TYF"
     gene            complement(13511..>13735)
                     /locus_tag="PLI76_05465"
     CDS             complement(13511..>13735)
                     /locus_tag="PLI76_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase"
                     /protein_id="HOI61513.1"
                     /translation="AELSQFVLYPQGMEVETAEIGRGSLKWTELTPMQSPVQYYIVNS
                     LASLPMKRVTQAVLIEGRAILHAMGARAIE"
BASE COUNT         2470 a         4346 c         4290 g         2629 t
ORIGIN      
        1 ccggtgatcc agccggggtc ggcggtgcac cagtagatgt cgctgtcctt tacgtcgaag
       61 atgtgctttg tcgtgtagta ggtcccgacc atatagccgc cgcaggcgtg tacgatgccc
      121 ttcggggatc cggtggtgcc gctcgtgtag aggatgaaga gcggatcctc ggcatccatc
      181 ggctcgggcg ggcattcgcg ccccacgccg ttcatcaggt cgtggtagtc tacttccatc
      241 tcggggtgga tctcgacggg tttctcggcg tcacgccgga ggatgacgat ccgctcgacg
      301 ctcggggctt tgacgaccgc ttcctctacg atgtttttga gcgggattgc tttgccgcgc
      361 cggtaggtga agtcggcggt gatgactacc ttcgcggcgg tgccccggat acgctggttg
      421 agcgcgctga caccgaaccc accgaagacg accgtgtgga tggccccgat gcgggcgcag
      481 gcgagcatcg cgacgatctg ctcggggacg accggcatgt agatgcagac ccggtcgccc
      541 ttcctgacgc caaggcggga gaggccgttt gcgaaccggc agacctcctg caggagctgg
      601 cggtaggtga ggatacgctc ctcgtcctcg gtctctcccc gccacatgag ggcgaccttg
      661 tttcgccgct ggctgtgggc gtggcggtcc aggcagttgt gggtgatgtt gagctttgcg
      721 ttcacaaacc acctggcgtg cgggtattcc cattctctca cctggtccca gggctggaac
      781 cattcaagtt ctctggcggc actatcccag aacccatcgg ggtccgcaag gaacgcctgg
      841 taggcacggt tgtagtcgcc tatccaggac tgctccctgc agctggcttc gggagtatag
      901 tacttcgcct cattgagcct gacgttaaag ttttcagcca tcctattacc ccaattatac
      961 attattaata atgtattttg tatatcaaca ttatggtcga gggaggtgtt cgggcacgaa
     1021 cgaacgcctc ctgagagcgg ataccggggt gcgctccggt tggcggggtg attatgccgg
     1081 agaggcggtg cgcgggggcg gtgccgggat gcgggcgaat cgggcccggg agcgggctcc
     1141 tacggaggag gagagacctg tccgccaggg gtttgggcct cgcggtgcgg tcgtgcccat
     1201 accgtctccc tccccggtgc ggcaccgtct ctacgatccc gccggccccc caaaacctca
     1261 cttcatcggc aggatgccct gggaccgcag ccaccgtatc tccacgagaa cgtagatcga
     1321 atcgtactca ttgcgcgaca acctccggtc gaacaacgac tggtagagct ccatcctccc
     1381 ggatttagca tcttccttat acctctttgt ccccgcgtcg tatgccttct caaaatcaca
     1441 agaactcacc cacgtcgtct cgagggcatc gacctccgtc ccggccttat cggtcttcca
     1501 tgcgacgtag gcgtgacccg ggacgacgac gatgtacggt tgcataccga gccgctctat
     1561 cgccgaggcg aagaggacgg caccgtcgat acagtttgcg ctcccggttg caagcgactc
     1621 gtccggggtg ctgacccgct gcgagtcgcc catgccgaac gctaccagcg cgtcgacgta
     1681 actcaggtta tagtcgtact tcagcgcgtt gtagatggct ttgacctgca gcgccgtccg
     1741 attcttcgta tcggcgtagg ttgtccgttg gttgggaagg gcatagcccg gcagcgtccg
     1801 ctccataccg tagtccacat acctccggtc gaactccccg gtcgcccgct cctttgcctt
     1861 tgccgccagc gctgccaccc cgggggactg cggggtcacg aatatggcga tgagcccgtg
     1921 atagatcgtg atcaccccgt ccttgtcctc cgtggcccac accatctggt ccatcgggta
     1981 gaacgtgacc gggtccgtct gctcgtcgtg aacggtccag ttgccgccat cccagtactc
     2041 gatccggtag tggacggcta ccctggccgg cgcccggatc tcctggatct cctcgcggtc
     2101 caggacgacg aaatggtcga acgtggccgg gctcccggcg ccgatctccg atacgctgac
     2161 ggtcggtgcg ctgtagcccg ggtactccga tacgagccgg acccagacgg gctctgccgt
     2221 cgggtttcgc acggtatacc ggacgtccgg tacgttcatg aaccatgtag gcctcaggtt
     2281 gtgccaggct aaaggataat cggttcggtg ctccacggag gtccggagag gggagcccgg
     2341 gtgccccggc ggcaggtccg cggagccatc ggcccccaga acgggaagag aacaggatac
     2401 gagcacgatc aacatcacca gccagcacac cacaacgcgg cgaagaaggg ttggtacgag
     2461 ggtcatacga tcggctgcag tgtggatgcg gtggcggtta aataccttgc ctcaggatac
     2521 cggccggccg catacgcctt cccgggcaga tcagcagccg gccgcatgca gtcttccccg
     2581 ggggagcgct gccgcctgca caagcgcccg ccaccgggcc ggacagcgcc ggaacatcgc
     2641 cccgcattca tatggatact attatattct gggatacata ttatgccccg gtgaagatcg
     2701 gcatgacccg gatctgccat atcgccggat ttttcctcct cgtaatcgcg atctccgcga
     2761 ccgtccagag cgtgcaaccc gccgatgcgg tccaggccga cacctcaaac cctgcgctac
     2821 aggcggttcc gggggcagat aacttcccga tcgtacagat aacaaacagc acgagagatc
     2881 aggttcttcc gcgcatatca ggcaacagga tactatggat ggagaccgat cgcatcaata
     2941 ccaggaacct gtatctctac gacattgcaa accgatccgc ggagatgatc atcgagcact
     3001 tttcgccccg gacatccccg gagatctccg gcaactggat cgggtgggtg gaagaccaga
     3061 gcgattcctc cggtaagatc ttcccgtgca tccgcgtgta tgacatccgg agcggtacaa
     3121 tcacccagat cacgcagtat cccgccatgc cgtaccagac gagcgagtcg caggatataa
     3181 catgggagcg ggagtcgacc ctggccatat cgggcgacat catcgtctgg cacgacaaac
     3241 gggacggaaa tatggatatc tactaccgca acctctcttc gggggtcgag aatccgatag
     3301 ccagatcggc ggccgacgag acctgtccct cgatatcggg cgatcggatc atctgggctg
     3361 aacagcacgg gacgaaaggc tataccatcc acctctataa cctgacgtca aacgagcacg
     3421 accggatcat cgatctcccc gccatgcgaa ggaaccaggt gatatcggga gactacatcg
     3481 tctggtcgga gtggagggaa gggagttatg acgtctgttg ctatgacatc gcatcgggca
     3541 acacgacctg gatcaccgat gagcccgggc acgatctctg gccggcggtc tccggcgacg
     3601 ccatcgtctg gtctgtttac gggagaggga gcgacgacaa tgggatttct gcgtatgacc
     3661 tctcgacgga gaccggaacg gagatcctcg ccgatcttcc ccacccggtc tggccggaca
     3721 tcgacggcgg caaggtcgtc tggtccgaca accggaccgg caattacgac gtctacctct
     3781 gctcgctgga gaaccgatcg gatgccgcgc ccgggctcta taccgttcac ctcaactcta
     3841 tcccctccgg agccgatata tctgtcaaca acgagccgta tggtcgcacg ccggcgacgc
     3901 tctccttcga tcggacaggt atctgcccca tcgggctgaa aaagaatggt ttcataccct
     3961 atacgacgac gcttgacata tccgcatcgc tgacgttcgt ggtgaacctt gagcgggagc
     4021 tggtcggccc ggtaagcgga cctcgcccga tactgatgag cataaccgta gactcagtcc
     4081 cgcggggcgc aaatgtctcg gtggacggag aggtttacgg gaccacgctc tttgggatgg
     4141 acaggctccc ctcgggggac cacgaggtcg agttgaccct tgagggctat cagtccaacc
     4201 gcacgaccgt gaactcatcc gaaccgatca acatcaccct gattcccgga acggacgccg
     4261 accggcctct gttgatggaa agatacgcct gatgttctcg tacctctcga aaaggcaggc
     4321 atccaccgcc atcatcctca ccctcctcgt tttcatgctc cattatgggt tcagtttact
     4381 gtttccaggg tatctctacc tggaagaagc atccgacctc atcgcaatcg ctgcacagag
     4441 cggggcgatc gggttccagc tcgccggcgc ccggtatctc gtcgcccggc tgaagaggac
     4501 gtttaaggat gagtttccgg cagttcccgg gcgtgggggc ggcgattgtc cgtatatggc
     4561 ggcaagaagc gttctctgca acagggcaca ttacatcgcg ttcattgccg ctttcctctc
     4621 gccgttcatc gtcatcacgc tcgtcagtct ccagccggac agcgcacccc gccacctcct
     4681 ctctcttgcg agcagatcga tccccggcct caccttcgac gtgttcaact acgggacggc
     4741 gtatgccaac tactggctgc tcggcacgat cgcatggatc ctccttggcg ttgtggttgc
     4801 gctgaacggt atggaaggtg ccggatgcag cgtatacgat gccgtcgata tctacagccc
     4861 cgaccggatc ggaggcctct caccggtcag gaggtacctg atgagcgctc tctccatctt
     4921 cctgataagc atcgccctgc tggtgatggg atacatcaac ttactggaga gtttcatcgg
     4981 ctccagccag ggattattcc acttcttctt cgatgtggtc gcagagatct gtgtattctc
     5041 ggttctctca ctctctggtg tcgctctcac cgtcgtcggg ctgaaccggc tctcagccat
     5101 ctgcctgaaa aagatcgaag accgggttaa cgccgtaaac gagaggtgcc gcacgttcca
     5161 ggagaacctc ttctcccttt cgcccgacga actggagaga cggggagacg aggtcggcag
     5221 cctgaaactg gtcgtcgagg ccctcagcga tgagcgggaa cggttgctgc agcggtattc
     5281 cgactgcagc gggttcaatg ccaggatggc gctcaggatc tttgtagcgt atatcccgcc
     5341 gctcgtcacg atagcgttcc aggtgtcgca gttgctgcct cgattacggg tctaacaacc
     5401 gtgacggaga ggcctgagcg ggccggcagc gtgatcctga tgcccgtgcc ggagggtgtg
     5461 tgtcaccccc gggagtcctc ctctgccatc cgcgctgccc gcattgcctt cgcccgatcg
     5521 atgaggtact caagaccggc aagttcgtcg gcgatacccc aggtctccct gcggcgggcc
     5581 tcgtcctgca gccggcggca ctccccctcg atgcggcaaa ggtcgtccat cgtgtagcgg
     5641 tgataccggg caagtatgtc cccgtactcg tcggcgaacg cggctatctc cgcgataagg
     5701 tcaatctggc cctttcttac ggcaccgggg ctcctatccg ccccggaagc ggtatgctcc
     5761 atactcacat ctcgccgcgg gaagggttat accttccaga gcggtcagcc ccggcggagc
     5821 cgggagaaaa accccgtaac gccgcccgag acgacgaagg ccacgccctg cagccatacc
     5881 gtaaagaagt cccgggcgac ggatgtaagc gcctcatccg ggtacatgct ggcctgcacg
     5941 ttccggagcg agaagtacgc gagcagcagc catacggacg cgacgagcgc ctggccgaag
     6001 accgatatcc agagcccgag ggtggcgaat accatggcca gggtatagct gagcacgctc
     6061 gtgaagaagg ccgagaagca gttcagcacg acgccgcgcg acaccacgtc gcggagctgc
     6121 ggcaggagca tgactgcaaa gagtatcgtg attccggtta tcgcaaggtc ctgccaggcc
     6181 gtatcgatca catccgtgct gcctgcagat atcgacgtcc ggacatatca gatcggcaga
     6241 tccggatcgg gttttcgccc accgggtccg gtcgggccag aacggcgacc gcaaatatga
     6301 gcggagtgcc gtaggccggg gctcacgcca ccgccttcag gtactccttc tccgcggcga
     6361 tcgcccggtc ccgccagccg gtatcgaccc acgtgtgctc cgcaaaccgc gaggatagga
     6421 caagcccggt gccgccgttc tcgagcgtcc agacctcctg ccccggtgcg aaccggcccg
     6481 cgatctcgtt tgccaccgcg tggtagacga tcgtgtcgat ccccttcacc accgacgcga
     6541 cgacgaggtt cggggcgacg taagactggt cggtatccac gccgatgaga aagatatccc
     6601 cggtctcccg ggcggcatcc acgacccccg tgcccgaagc accggcagcc atcaggatga
     6661 cgtcgacgcc atcggcgcgg aggtcccggg cgagatcacc cgcgcgttcc ggcatgccga
     6721 acccctcgaa actctcgccg acgtagagga ccgtgacgtt cacggacgga tcgtatgccg
     6781 ccgccccccc ggtgaacccc ctgacgaagg gctcgatgac ggcgaccggc gccccggcga
     6841 tcgcgccgat atgcccggtc cgggtgatat tcgccgccag cacgcccgcg aggtaggatg
     6901 ccccgtaggg gaccatcacg acgtcgcagg cgttcgggag gccgtagtcc tcctgctcaa
     6961 gggtgacgaa ccggatatcg gggttcgccg cggcccacgc ccgggcggct ccggtgaact
     7021 caaacccctc ggtgatgaca aggtcgggct tctcggtgaa gttttgtaga gagaagacgg
     7081 cgtcgaggag cgcaaggtcg gcgtaggtga actctctctt cgtgaacgag aacgattcct
     7141 gggcgcggaa gaggccgcgg tacgccgagt cggcaaacga ccggtcgccc ttttcaagcg
     7201 ggtagatcat ccagacgacc gggcggtcgc cggcgctgcc ggcaaaggag aggtcgggaa
     7261 tgtacgggtc gatcgccgcg gacgggaccg ggctcccctc cgagaacgcc ccgccctcga
     7321 tcgcccagat agagtagtag ccggcgagcg gccctctgga agagtgctcg ccgaaccgga
     7381 ggggtcccga tgcgccggtg accgccggat gagcgcctgc ccggagcagc gccatggcct
     7441 cgccggcctc ctccggatcc cagcccttga cggtgccgtc gccggtgacg acccagcgga
     7501 tcgatgccgc gagcgactcg cccggggccg cctcctgccg ggccgcggtg tagagggcga
     7561 ggaggaaggc gtcgtaggtc tgtgcggcaa aggccggcgg tgcttcgccg aagatctcct
     7621 cgtaggcgat cgcgtagccg gtcgaagggt cgggggcaag cgacgtgccc cggataccct
     7681 ccgcccgctc gccgaggtgc tcgataaggt aagacgagcg gcaggcgtca gagaagaaga
     7741 ggcggtcggg agaaagaacg tttacaagcc cgatcgcctc ttcggggtag acggcggcaa
     7801 cgacgatgtc gggctcctcc ttcacgatct gccttgcgat ctccgagagg tcgtcggatg
     7861 cgctgagcga tgccgctccg gttatccgga tgccgttctt cgcggccgcc tccggggcaa
     7921 gccgtgcaaa cgtctccccg tagggcgtag ctcccgtgac gagcgagacg ttcctgacac
     7981 catccccgcg gaggcgcaag aagatggtct ccacctgccg gccgtcggcg aggcaggttc
     8041 tccagaggag atcattatcg ttgagctctc ccgtggcgag ctccatcgtg acgctcgggg
     8101 atatgaggag tttgccccga cccgtgacga ggggagcgat atcgatgagc tccgcgctca
     8161 tcgcggggcc gatgacgata gcgatgtcgt tgcgcccggc gagctcctct gcgtatgcag
     8221 atatgttgcc ggtgctcgta tcccggcaga cgagttcgat cgtgcgcccg ccccgccggt
     8281 tcatcgcatc cgctgcccag gcaagccctt ccagagagtg cagggcaaca ggcccatctg
     8341 cgggcagcag gacgccgata cggatggggt cggccgcggt ggcggtgcac cccgcagaag
     8401 agatcgccag cgtgacgata cagaagagag cgagagccgg aataatcctg gtcctataaa
     8461 aaagcagtcg tgaggtcatg ataacgcccg gacgccgccg gccggcggct gatcgttgta
     8521 cttcggcgac acctcgaata tgctttttca tcgggctgtc cggaggaagg agagcctgcc
     8581 ggggggtttc cggcagagga ccctcacgcg gcctttgcta ttcgcgcttc cgccttcacg
     8641 ccgaccggtt cggggagtgc gcccatgtag gtgaccagcg catcgatatc gagcgggcca
     8701 taggccgggt tcactccttt cgtgaagacg gtatacccgt cgccgccggt tgcgaggaaa
     8761 tcgaccatcg ctgccgtata gttcgcgccc ggctccaggg gggcgccgcc cacccggatc
     8821 tcggtgatct tttcgcccgc gggcttcccg tcatcgaacg tgtaggagag ccccgagacg
     8881 gcgaggttat gaggcggcaa cggggtctgc cactgctgtt cgagcacatc ccggatctgc
     8941 tcccccgtga gggtcatcga gaggacggtc gaggcgaacg gctgcaccgc gtagaggtcg
     9001 ccccaggtga cgttgccctg cggtatgtcc gcccggagcg aaccggtggt gatgaaggcg
     9061 acgtcggcct gcatcgccgc ccgctggccg tcggcgacga ggttgccgag gggggattcg
     9121 ccggcaccgg tcgcctggcc cgtgatgctc gccgatgcgc tcgtgacgac ccgctccgtc
     9181 agaggcctga ctgcatctct cgccctctcg aggagtgcgg ctgccccggg atcggtgccg
     9241 gacaatcggt tcgcgtatgc cgggacgatc tcggccgtcc tgtgggtgac gtcgccggta
     9301 gcgggatcga tgacgagctc gatgtcggca aacgctttgc cgtaactgta tgcctgggtc
     9361 accagcaccg atctaccgcc ggcattctcc aggcaggcgt tcgtgaactc atgggtgtgg
     9421 gccgagagga caagatccac gtccgggtcg aggccggcga cgatcccggt gatcctgccc
     9481 gtcacgttgc cgccttctcg tgtcggaccg tcataggcat cctgcgaacc tccctcgtgg
     9541 aggaggacga tgatcgcgtg cacgcccttc tcctggatct cggggatata ccggttgatc
     9601 gattcggcct cgtctttgaa gacgatatcc tcgatgttcg ccggtttctg tatggaaggc
     9661 gtctcgatcg ttgtggcgcc gatgaacgca atccctacgc cgccggcatc gcggatggtg
     9721 tagggggcgg cgatgggggt gtcattcact ctccagacga cgtttgagga gacgtactcc
     9781 atagccgcgc cggggtaggg gtcgaccagg tgggtgatgg tcgtcgcgcc gtttccgccg
     9841 tcgaccatcc gcatcagttc gtccgtgccc cggtcgaact cgtggttgcc gaacgttgca
     9901 atctgcgggc aatcgcctgc aaggccgttc cagaagagaa tggtgggctc gtccagcagc
     9961 aggccggact ccggcggcga tgcgccgacg atgtcgcccg gcaacgctat gaacgtggcc
    10021 ggccggccgg ctgccgcgtt gaggtacgat gcgagcaccg gcgcgctgcc gaccggttcg
    10081 ccgctgaggc tctttccggc ggtcagctgc ccgtggaagt cgttgaccgc gagtatcctg
    10141 acgtgcacgg gcgccgggtg cagataccag gagagcccga tggggatgag aacgatgatc
    10201 gtgagcacga gaaagccggc gatcacctgt aaccatggcg atttgtcgtc gatgggcgaa
    10261 ccggatgtca tcaggaaagt tctggcctga tcgggataaa cccagctgga atggatgcgc
    10321 acgagactgc ggcgcggccc acgatcgggg ccggataccg cgatcgatag caagacggcg
    10381 gagcaggttg ttcagggtac tcaatcgagc cccggttcct gcttcgcctc gtccgtcgtg
    10441 taaactctcc cggtcccgat atccgcaatt gcctcctcga catcacggat atcttcatcc
    10501 gtcgttcccc ggcggtttcc gggtcaaccc ggccccctct ccccctccat cctcttcgcc
    10561 ttcatctcct ttgcccggcc gatgaggtac tcgagctccg caagttcgcc ggcagtcccg
    10621 caggcgcccc tgctgcacgc ctcgtcgtgc agcctccagc actcctgctc gatccggttg
    10681 aggtcggcca tcgtatggat gtggtaccgg gcgagtatat ccgcgtgttc gcggacgaac
    10741 gcggcaatct cttcaaggag ggcgagccgg tcccccttcg gcgaccctgt ggcgcaccgg
    10801 gccggccggg aagtcgtgcg gtccatgtta cgtcatcgct gccgggaggg ttagtctttc
    10861 cgaaacggcg gggagcaatt ccagggtggc gggaaaaaag caattgagat gggcggggga
    10921 ttcgcacatc ccgcccccgg taatctccat ctactacgat tcgtggacga tacccatatc
    10981 gacgccgacg agcccgacga tggtgccgac cgcatccctg atcggggcat acccggtgag
    11041 gacggtggcg gtctcgttgc cccagtcgtc gacatagaac ccgggttctg cggaaggtcc
    11101 ctcaaagccg gcgaggaacg cggcgtcctc ctccgtgggc acgtaggtat agcctatcgc
    11161 gggcgcgtcg ccgttgccgt agtccgcatc gacgacgtac tcggtggcgt tcccggtctt
    11221 acgcatggtg tagacgtaga cgaccccggg ggttgcgtcg cggaaggcgt tgaggtcgtc
    11281 gcggatggcg gtgaacgccg gtgtattctc gtcgccgggc ctgagcgccg ccatcgcggt
    11341 gccgtcgata tcgcctgcaa cctccaccgc aagggccttc agcctctcct ccgtgatcgg
    11401 gttcccgact tcgacggcca ccataccgac gccggcgccg ctcgtgctct tcacgggagc
    11461 atacccgcag atgaggctgt agatctcggc cgaaggtttc tcaaagccac gaaggaggac
    11521 accatcgatt gccgtcgggg tatacccgtc gcctatcgcg actccatcgt cgctgccgta
    11581 atccgcatcc acgacgtact cgacggtgtt tccggcttta cacatcgtgt aggcgtaagc
    11641 gatctcaggg tgttcggccc ggaacgcatt gagccgatcg tagatggcaa cgtacgccgg
    11701 cgtctcctca tcgccgggct tgagggctgc catggcgtcg ccgtcgatct gccccgcaac
    11761 ctccactgca agggcctcga gcctctcctg cgcgatcggg ccctccggtt cgaggcagac
    11821 cattccaccg ttcttcaccg gagcataccc gtaaatggcg gagtacactt ctgcggaggg
    11881 tttctcaaac ccggcaaaga gtggagtctt tgggtccacg acctggtgtc ctccgccgat
    11941 agcgcgtgca ccggcgttgc cgtagtctgc gtcgacgacg tactcgacgg cgtttccggt
    12001 cctctgcagc gtgtagatat agatgatatc aggatgctga gaccggaagg cgttgagccg
    12061 gtcgtggatg gcggcgaatg ccggtgtact ctcgtcgccg ggcttgagcg ccgccatcgc
    12121 atcgccgtct atctgccccg caacctccgt cgcgagggca ctcatcgcat ccagcgggga
    12181 cgccttctct cccggcacgg tctcgccggg agcgaacgct atcatgatcc ccgcaacaac
    12241 ggcgatcaac gcaacgataa cgaatatcag aacgttctgg cctgtcatat gggatacact
    12301 ctttcaggat ttattgcagg agtatgtact taaacgctcg caaatgtatc gcggctatgc
    12361 atcgccgtac agagatttag atgtcggatt gtcgagcatc caggagaaga acggaaaaca
    12421 gggggaagcc gggagtgata gcggcgagcg ccccgccggg gcaggcaaac gccctccggg
    12481 accggccgat attccatggc ggcgcgctcc ggtagaccgc gtcgaagatc cacggtagcc
    12541 cgggagcggt tcagcctgtc cttccgtcgt tgccgggcgt ccggtgacgc aggggcgaca
    12601 ccggatcgct gccggccagg acggagcggc accatcgcac ggcgatcccc gacctatcag
    12661 aagtatgtcc cgcctttcgc aaacgcttcc cgccgctcgc gtgccatctg caatagccgc
    12721 tcctcaacct ccggattgcc gaggacatac cctttccaga ggtcggtgta gggttcctcg
    12781 ggcacgacga tgatcccctt atgctctgcg acccgatcca gtatgagcga cgccgctttg
    12841 tcgacggggt atgcatcgtc ggggatcctc agctcgccat gggcctgccc gtcgatactc
    12901 ttgttgaaga tcggtgtcgc gatgttcgcc ggacagatcg tggagaaata gagccctcta
    12961 tccgcgtact cgtacctcag gcactcggtg aggccggtga cgccgtactt cgtcagggag
    13021 tagagcgcct ggaacggcgg cggagcgatg cccgctatcg aactggtgtt cacgacgtgc
    13081 ccgaacccct gtttgagcat gatcggcacg gcggcatgga cgccgtagat gacgctccag
    13141 atgttggtat cgatgatcgt cttccagtcg tcgagggtgg ccttctcgaa cgggatggtt
    13201 cccccgacgc ccgcgttgtt gaagagcagg tccagcctgc cggcctctgc cgccgtattc
    13261 tcgataccct tctgtacctg ctcctgcttc gtcacgtcca tgatgagcgg gcgcacccga
    13321 tcagccccgt atgcggagag ctggccggcg gccgcggcga ccttctcccg gctgcggccg
    13381 gccatgtaga cgattgcccc ccgcttcagg agctcttcgc tgattgcata ccctatcccg
    13441 gagttcgcgc cggtgacgat gcaaaccttg cctttatagt aatccggatc gaccataatc
    13501 ctcgcctcgt tcattcgatg gcccttgcac ccatcgcgtg aaggatggct ctcccctcga
    13561 tcagtaccgc ctgcgtcacc cgcttcatgg gcagggatgc gaggctgttg acgatgtaat
    13621 actggaccgg gctctgcatc ggggtcagct cggtccattt caggctgcct cttccgatct
    13681 ctgccgtctc cacctccatg ccctgggggt agaggacgaa ctgggagagt tccgc
//
LOCUS       DAOSEE010000052        13418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1793_length_13418_cov_240.138816, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000052 DAOSEE010000000
VERSION     DAOSEE010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 13418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13418
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1793_length_13418_cov_240.138816"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1650
                     /locus_tag="PLI76_05470"
     CDS             <1..1650
                     /locus_tag="PLI76_05470"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOI61514.1"
                     /translation="TPTASGKTLAFNIPIFEILVNNPRATALYIYPIKALANDQYKTL
                     KEFEAATRIIVNAERFDGDTPGDERDRIRCTSKVILTNPYELHYMLGTHRRWASFFAG
                     LKCIVVDEAHTYRGVFGSNVALVFRRLERICAHYGSDPQFILSSATIANPEEFGGKLV
                     GRPVVHISRDGSPRGSKHFVLYNPKPEGAKEDTVALSVHNETVKLLAACVRMETQTLC
                     FARSRQLVELVALWTKEALRDDYPHLAQRVRSYRGGYLPEDRLRIEQQIKDGSLLGIV
                     STNALELGIDIGSLDCVILSGYPGTMMSTWQQAGRAGRRATESVAIMVAYANPLEQHF
                     TRHPEDFFARPHENAVLDLENRYIRADHILCAAQELPLEPGRDREAFGDTIMETFREL
                     AARGDLRQTGAGWTYAGSETWPHGSVRLNSISSDSFLLMHGHRLLETLDPAQAFREAH
                     PGAVFLHDGQTYIVDEFDPEKRVIRVRYEETDYYTRAAREVSLRVTRPGARTRAGSID
                     IHTGDVVISESYTGYRVIKHEKVINEISLDLPPLHFETRAT"
     gene            1735..2067
                     /locus_tag="PLI76_05475"
     CDS             1735..2067
                     /locus_tag="PLI76_05475"
                     /inference="COORDINATES: protein motif:HMM:NF020926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HOI61515.1"
                     /translation="MPTILPYFIMCDARDINVHAGYRDAETGAPTVYVYDNFVGGIGL
                     SEKVAGLLPDILAMACRLVADCRCESGCPSCIYTSSYMSEGDVDKRATRVLLERLRDT
                     HLQAQIPS"
     gene            2259..2948
                     /locus_tag="PLI76_05480"
     CDS             2259..2948
                     /locus_tag="PLI76_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61516.1"
                     /translation="MKKTQQKTPKGDDKAWLKWGYVGIALLIAVAMVGTYFAPVFGSS
                     QKAQIGNNAAIGYTIRGEDGRPIITTDQNLFSSEYEKGNLVLLTRGLTMPVGGLVSGE
                     NVATLPVEYPPDFGDFGLLGFETNAISEGLVGMRPGEVKVVSFSYGENNLEMNLSQED
                     VDGIGLNFTERQVGDVVALGLTTSPDISMGNDTATPALRFGKVTEKTADSLTIVYRYG
                     SAEITLNSITG"
     gene            2994..3272
                     /locus_tag="PLI76_05485"
     CDS             2994..3272
                     /locus_tag="PLI76_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOI61517.1"
                     /translation="MAIKVINFYQEGCMGCEEQAPILHEVEQNLGITIEEIDAVKNPH
                     YIKEYNLRVTPTTLVIEDDRVRERMEGLVHREDLETAIRRHLPRPLPG"
     gene            complement(3553..4143)
                     /locus_tag="PLI76_05490"
     CDS             complement(3553..4143)
                     /locus_tag="PLI76_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HVO_0476 family zinc finger protein"
                     /protein_id="HOI61518.1"
                     /translation="MMSIVCPACKEECEHQVLREAAELVVQCSVCGQIHRIPKPPEPR
                     IFTVKAIVSRETESQVCSVEMLEDEVVSLGDHIAAECGDEVYGVEVTGIEAGEKRVRR
                     AKAEEVTTLWTRGIEQVVVKASIHSGRTTIPLYQAAEGEDEFVVGEVYTFGGRRIRIS
                     HIKLRDGPVMRKEGWKTVASRVKRIYGYLEGRYPRT"
     gene            4177..4602
                     /locus_tag="PLI76_05495"
     CDS             4177..4602
                     /locus_tag="PLI76_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF371 domain-containing protein"
                     /protein_id="HOI61519.1"
                     /translation="MKARDIVRARGHPLVRGAHPTTFEVTRDETLTAAGDCIIGIGAD
                     KGAADLDPGLKAVLRDGRAVLTTRLTAGGVTVEVRSRGSAALTLDHPADLVWRRSDFI
                     SDRTVGIRSDHTAATLPREFIEALRRGEDLVVELEAESP"
     gene            4721..6619
                     /locus_tag="PLI76_05500"
     CDS             4721..6619
                     /locus_tag="PLI76_05500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOI61520.1"
                     /translation="MHPPTPALDDLLEALDHRIRAVGEDAAPGGDLPGSGLCDLIDTL
                     SARIRSIDAGYRRCRHRLALLPAGCLCTGPDGVILEANRAAGALLGPAPDRLRGLSFP
                     AHLHPGSLSAFRSAVAALERGDEVPVQEFLLARPDGSTLPVGAAVAVVRDGAIEHLWI
                     LSDASGQKMIEEARRRSEERYRELTGNISDAFLALDQEDRIISWNRVAANLSGHPGEE
                     VIGKSIYDVYPELRTEEVVGFFREVRETGRPGMSECRFHVGGRDHAFEVRAVPTREGI
                     SIYIRDITDRRNAEEALRKSEERYRAIVENQTEMIYRRLPDGTITFANDAYCRSIGIP
                     PGDLVGRRYAPAIPADDQARIQKSLHSLTPAHPVLAVEHRVVMPDGEIRWQQWTHMAF
                     FDETGSLVEYQSVGRDTSAARMAEEALRLANKKLNLLSDVTRHDTLNRLNVLTGYIDL
                     SREETKDPDLQEYYRKEGEIVREIRRYMVFTGEYREIGVAAPIWQDVGRIAREAAAGL
                     DMGEVAVEVQVADIEVYADPLIVQVFANLIDNSLRHGGGVARIRISARESDRGVTIIY
                     EDDGIGIPLAEKEDIFERGFGLFLAREILSITDLSLRETGRPGEGARFEIGVPRGYYR
                     FTGPGQAP"
     gene            complement(6594..6779)
                     /gene="xseB"
                     /locus_tag="PLI76_05505"
     CDS             complement(6594..6779)
                     /gene="xseB"
                     /locus_tag="PLI76_05505"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOI61521.1"
                     /translation="MTKTFEEMLEELRGIVRRLEDGDASLEESISIYERGALLVKQCE
                     DLLGAAEMKLTELGRDR"
     gene            complement(6763..8007)
                     /gene="xseA"
                     /locus_tag="PLI76_05510"
     CDS             complement(6763..8007)
                     /gene="xseA"
                     /locus_tag="PLI76_05510"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOI61522.1"
                     /translation="MLSGPAADPDQPGGLIFGVSEVSTLICDLLDDARLQEIWVRGEV
                     TNYKNHSSGHHYFSLSEGGERSSALINCVMWRSCAAELRFAPRNGMGVLAFGTLEVYE
                     PHGRYQLIVRRLLPAGAGERHLMVERWKRELDAEGLFSPERKRPLPAFPRRVGVVTSR
                     TGAALQDILSVISRRYPVEVVLSPAAVQGEGAHADIAEAIRRIDGLVDVIIVGRGGGS
                     FEDLFPFNHPDVVRAVAACRTPVISAVGHEVDTALCDFAADLPAPTPSAAAERAVPDR
                     VDLLREVAGHRERMRALLSHRIAAAENEVDDLRARMHPRRLERRVHERMQRLVEHADL
                     LRRAALARVQRERAALAEARAELLGRDPLAALDRGYCIVGSGRRVMRSARDLHPGDRV
                     MLLMKDGRCRAVIEEVTYDEDV"
     gene            8050..8874
                     /locus_tag="PLI76_05515"
     CDS             8050..8874
                     /locus_tag="PLI76_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOI61523.1"
                     /translation="MPRFAVSTMFFHEYPCDHIFDYIAESGLDSLEFWVETPHFWLAG
                     RPEGDLSCCIADHPDLSPITIHAPSLDLNPCSINPEVAAISVDYAVESVRMADRMGAA
                     VVTVHPGRRTAKRPPSAYDYQRFEKYVARLREAASEARVKVAIENLEPRVNALLTTAE
                     DAAEVLEREPWLRFTLDMGHAMMTSCDEVLRFIDLCIERMVNVHVSGLGENGRPHYPI
                     HDDPDAMRVLTELADRGYDGYLTLELEDMVFPGALSSEEKIVVLKRQLETFEKVFS"
     gene            8970..10250
                     /locus_tag="PLI76_05520"
     CDS             8970..10250
                     /locus_tag="PLI76_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOI61524.1"
                     /translation="MVIVDLVTVEIILFIICLLLSAFFSSSEVALISITRAKVRALLN
                     QGRKGAEALDVLKRSTDTILITILIGNNIANVAAASLATAIAIGIYGDVGIGIATGVT
                     VILMLVFGEIGPKMYASRHTESLALSVARPILYLSKALYPVLWISDRINRQFAFRPGV
                     TEPVVTEEEIKEWIDVGEEEGTIEEQERDMLYSVLRFGDTTVREVMTPRIDVVMIEDA
                     NTIEGALALFNETGFSRIPVYHEQIDNVVGLLNVKDVFAAVVRQQTSAMIRDVMYEPY
                     FVPESKKIDELLKELQVKKQHMAVVLDEYGSFAGIVTVEDMLEELVGEILDEFDEEEP
                     EVQRVEENVYLVDARAWVDHLNEDLHLALPLTDAYESIGGLVIDRLGHIPRRGEVVKL
                     EESNITLVVMQMRGRRLVKVKLIVAPPPAPEESR"
     gene            10264..10875
                     /gene="purN"
                     /locus_tag="PLI76_05525"
     CDS             10264..10875
                     /gene="purN"
                     /locus_tag="PLI76_05525"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOI61525.1"
                     /translation="MERKKRIATLASGRGSNFQAVIDAVAAGDIPAICVGLITDNPQA
                     YAVERARAAGIPVTVVDYARFPSKAAYEEALLAAMRGCRADLFVLAGYMRILGAAIVH
                     EFSGRMMNIHPALLPAFAGLHAQRQAIEYGVKVSGCTVHLVDEGMDTGPIVVQRCVPV
                     LSDDDESALADRILAEEHRALPLAVKLFCEDRLEVAGRKVRVR"
     gene            10944..11261
                     /locus_tag="PLI76_05530"
     CDS             10944..11261
                     /locus_tag="PLI76_05530"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component 4"
                     /protein_id="HOI61526.1"
                     /translation="MADTTRRSGSRRLARERIAILFARAAEFYPDNPAWSNRCVELAR
                     KIGMRHRVRIDRPLKRRFCRRCSVYLVPGANARVRIYRGRVIVTCLACGHRSRYPVGR
                     RTS"
     gene            11258..11518
                     /locus_tag="PLI76_05535"
     CDS             11258..11518
                     /locus_tag="PLI76_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HOI61527.1"
                     /translation="MSRESFQDIKPTVWVGKRGISSLMIDEIRRQLETRKIVKVRWLR
                     NTEVDPEEVAVSAGAVLIEVRGRTFVLAERRSRSPGRNSRNI"
     gene            11570..12019
                     /locus_tag="PLI76_05540"
     CDS             11570..12019
                     /locus_tag="PLI76_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19e"
                     /protein_id="HOI61528.1"
                     /translation="MTTVYDIPADMLIRQAAEELKKNPAIQPPDWADFVKTGVHKEMP
                     PENDDWWYVRAAAVFRRIYTDGPVGTQRMRSIYGGKHDRGSAPSRFKRGSGSIVRKIF
                     QQLEAAGYVAHTSEGRMVTAAGRSFLDNVANNVKSQVSGISPGIAKY"
     gene            12037..12375
                     /locus_tag="PLI76_05545"
     CDS             12037..12375
                     /locus_tag="PLI76_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOI61529.1"
                     /translation="MVDDELAELRRRKMEQMQRQAMDQQAMEDEVERQRQIESQIRVV
                     LMEILEPEARERLNTIKLTRPEFAKAVEQQLVVLAQSGRVRQRITDDQLKALLTQLTP
                     SKKEFRITRK"
     gene            12375..12965
                     /locus_tag="PLI76_05550"
     CDS             12375..12965
                     /locus_tag="PLI76_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha hydrolase"
                     /protein_id="HOI61530.1"
                     /translation="MEAGLLFSGGKDSALAAVMLARDYGVELNTCVFDPARDVPAVRA
                     AAAALHLPFRRRVLGRDLLEEAADMLLVCGYPNDAITMVHRAAIEALAGEYRVVGDGT
                     RREDRVPRLERSAVQHLEMTTGSSYVRPLLGYGKPEVERLAGRLLVVRYGETGSIGNG
                     DYEQEIREAIRARGIDPAPFFPAGHLQSLVVGMRAT"
     gene            12992..13144
                     /locus_tag="PLI76_05555"
     CDS             12992..13144
                     /locus_tag="PLI76_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L39e"
                     /protein_id="HOI61531.1"
                     /translation="MSKLMKGRKIRLAKACEQNRRVPAWVMIRTNRAVSSHPKRRNWR
                     RSSLKV"
     gene            13149..13412
                     /locus_tag="PLI76_05560"
     CDS             13149..13412
                     /locus_tag="PLI76_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31e"
                     /protein_id="HOI61532.1"
                     /translation="MAEALKEHIYIIPLREVKRSPRWKRGNTAIKDIRAFLERHMKSA
                     DVKLDRSINEKVWENGSGKPPRKIRVRAMKFEDGQVQAELAEE"
BASE COUNT         2583 a         4300 c         4255 g         2280 t
ORIGIN      
        1 acgccgacgg caagcgggaa gacactcgcg ttcaacatcc ccatcttcga gatcctggtg
       61 aacaaccccc gcgcgacagc gctctacata tacccgatca aggcgctcgc aaacgaccag
      121 tacaaaacat tgaaggagtt tgaggcggcg accaggatca tcgtcaacgc cgagaggttc
      181 gatggagata cacccggcga cgagcgcgac cgtatccgct gcacatcgaa ggtgatcctg
      241 acaaacccct atgaactgca ctacatgctc ggaacccaca gaagatgggc gtcgttcttt
      301 gccggcctca aatgtatcgt cgtcgatgaa gcgcacacct accgcggggt cttcgggtcg
      361 aatgtcgccc tggtattccg gaggctggag aggatctgcg cccattacgg ctcggatccg
      421 cagttcatcc tctcgtccgc caccatcgca aaccccgagg agttcggcgg aaaactcgtc
      481 ggccggccgg tcgtccacat cagccgcgac ggatccccgc ggggctcaaa gcacttcgtc
      541 ctctacaacc ccaaaccgga gggtgcgaag gaagatacgg ttgcgctctc cgtgcataac
      601 gagacggtaa aactacttgc ggcctgcgtc aggatggaga cgcagacgct ctgcttcgcc
      661 cggtcgcgcc agctggtcga acttgtggcg ctctggacga aagaagcgct cagggacgac
      721 tacccgcacc tggcacagcg ggttaggtcg taccggggcg gctacctgcc ggaggaccgg
      781 ttgcggatcg agcagcagat aaaagacggt tcgctcctcg gtatcgtctc gacaaacgcc
      841 cttgaactcg gcatcgatat cgggtcgctc gattgcgtca tcctctcagg gtatcccggg
      901 acgatgatgt cgacctggca gcaggcgggc agggcgggga ggcgggcgac agagtccgtc
      961 gccatcatgg ttgcctacgc aaacccgctc gaacagcatt tcacccgcca cccggaggat
     1021 ttctttgccc gtccgcacga gaacgcggtc ctggatcttg agaaccggta tatccgcgcg
     1081 gaccacatcc tctgcgcggc acaggaactc cccctcgaac cgggccggga cagagaggct
     1141 ttcggggata cgatcatgga gacctttcgg gaactggccg cacggggaga cctccggcag
     1201 accggggccg gctggacgta cgcggggagc gagacctggc ctcatgggtc cgtgagactg
     1261 aactcgatat cgtcggactc gttcctgttg atgcacggcc atagactgct tgagacgctc
     1321 gaccctgccc aggcattccg tgaagcccac ccgggtgcgg tcttcctcca tgacggccag
     1381 acgtatattg tcgatgagtt tgaccccgaa aagagggtga tccgggtccg gtatgaggag
     1441 accgattact acacccgggc tgcgcgagag gtcagcctcc gcgtgacccg acccggtgcc
     1501 cggacccggg ccggatccat cgatatccat accggggatg tcgtcatcag cgagagctac
     1561 accggttacc gggtcatcaa gcacgagaag gtcatcaacg agatctccct ggacctcccc
     1621 ccgctccact tcgagacccg ggcgacctga ttctcgatcc cggtctctcc caacgagccg
     1681 ggcctttccg gggccgccgg gtttgaacgc ggcctgagag ggcttgaaaa cgtcattccc
     1741 accatcctgc cctacttcat catgtgcgat gcgcgtgaca tcaacgtcca tgccgggtac
     1801 cgggatgcag agaccggagc gccgacggtg tacgtctacg acaactttgt cgggggcatc
     1861 gggttgtcgg agaaggttgc cgggctgctc ccggatatcc ttgcgatggc ctgcaggctg
     1921 gtcgcggact gccgctgtga gagcggttgc ccgtcctgca tctataccag cagttacatg
     1981 agcgagggcg atgtcgataa gcgagccacg cgggttctgc tcgaacgact gcgcgatacg
     2041 catctccagg cgcagatccc ctcttaaggg agcatgcagg ccccggagcc ggcgtggaag
     2101 agaggctggt gaggggcggt tcttccccgg gcaccgggca gaggctctcc ggcccgtgtt
     2161 tctggtgtgc ggccgggttc tcgtatccgc gaccccgccg gatgcctccc cgcccacgac
     2221 ggtactattt tatttcaccc aaagacgatt gtacactgat gaaaaagaca cagcagaaaa
     2281 ccccgaaggg tgacgataag gcgtggctga aatggggcta cgtgggcatc gccctgctga
     2341 ttgcggtagc catggtgggg acctacttcg ccccggtctt cgggagcagc cagaaggcgc
     2401 agataggaaa caacgcggcc atcggctaca ccatccgcgg cgaggacggg cgccccatca
     2461 taacgaccga ccagaacctc ttctcgagcg aatacgagaa ggggaacctc gtcctcctga
     2521 cgagaggcct gacgatgccc gtcggcggtc tggtctcggg cgagaacgtc gccaccctgc
     2581 cggtcgagta cccgccggac tttggggatt tcggccttct cggctttgag accaacgcca
     2641 tatccgaagg gctcgtcggt atgcgccccg gggaggtgaa ggtcgtcagc ttctcctacg
     2701 gggagaacaa cctcgagatg aacctgagcc aggaagatgt cgatggtatc gggctcaact
     2761 tcacggaacg gcaggtgggt gacgtggtcg cgctcggtct gacgacgtcg ccggatatct
     2821 ccatgggcaa cgataccgcc accccggcgc tccggttcgg aaaagtgaca gaaaagactg
     2881 ctgactcgct tacgatcgta taccgctacg gcagcgccga gatcaccctc aacagcataa
     2941 cggggtaacc cctaccccac agccttttat agtctccctg ctttctccca tatatggcga
     3001 taaaagtgat aaacttctac caggaggggt gtatggggtg cgaagaacag gccccgatcc
     3061 ttcacgaggt ggaacagaac ctcgggatca ccatcgagga gatcgatgcc gtgaagaacc
     3121 cgcactatat caaagagtat aacctccggg tgacccccac gaccctcgtc atcgaggacg
     3181 atagggtcag ggagcggatg gaggggctcg tccaccgcga agacctcgag accgctatcc
     3241 gccggcacct gccccgtccc ctgcccgggt agggcgccgg ctcgccctgt tctatcgtag
     3301 cgatcgctac ccctcccctc ccttcgcgcg atctcgaggg tatgagcgat gaaacggcct
     3361 ctcgcggagc ctggaacagg gagttaccgg tacccatcca ccgaacttcg cgcccttcgc
     3421 ggcaacgcgc gagctggccg atcatatgcg gtgaaacgcc tcacgcgcga tatacccggt
     3481 gcggacaacg gtacgaccca cgcattgagc gcgatatacc cggcggcagg agcatgccgc
     3541 ccccgcccct tctcaggtcc gcgggtagcg cccctcgagg tagccgtata tccgtttcac
     3601 ccggctggcc accgtcttcc agccctcttt gcgcatgacc gggccgtcgc ggagtttgat
     3661 gtgcgatata cggatccgcc gcccgccgaa ggtgtagacc tctccgacga cgaactcatc
     3721 ctccccctcc gcggcctggt agagggggat ggtcgtgcgt ccggagtgga tggaggcctt
     3781 caccacgacc tgctcgatgc cccgggtcca gagggtcgtc acctcctcgg cctttgcccg
     3841 acggaccctc ttctcgccgg cctctatgcc ggtgacctca accccgtaga cctcatcccc
     3901 gcactccgcg gcgatatggt ccccgaggct cacgacctcg tcttcgagca tctccacgct
     3961 gcagacctgc gactccgtct cgcggctcac gatcgccttg acggtgaaga tgcggggttc
     4021 ggggggcttt ggtatccggt ggatctggcc gcagacactg cactgcacca ccagttcggc
     4081 ggcttcccgg agaacctggt gttcgcactc ctctttacag gcggggcaaa cgatactcat
     4141 cattcattag caatagaagt cctcacctaa ttaagcatga aggcaaggga tatcgtgcgg
     4201 gcacgggggc acccgctggt ccggggagcc cacccgacga cgtttgaggt gacgcgggac
     4261 gagacgctga ccgctgcagg cgactgcatc atcggcatcg gggccgacaa aggcgccgcc
     4321 gacctcgatc ccggcttgaa agcggttctc cgcgacggga gagcggtcct cacgacccgc
     4381 ctcaccgccg gcggcgtgac cgtcgaggtc cgctcacggg ggagcgcggc gctcaccctg
     4441 gaccaccctg cggatcttgt ctggaggagg agcgacttta tatccgaccg gaccgtgggc
     4501 atccgctccg atcacaccgc ggccaccctc ccccgggagt tcatcgaggc gctccggcgg
     4561 ggagaggatc tggtggtcga actcgaggcg gagagcccct gacgggtgcc ggcataccgg
     4621 ggccgtaacg gcgcctgcga cgaagagcgc ccctgatggg caggatacgg gggacccggg
     4681 atagatttat ccactcaggg tgcgggggaa cgggtgaggt atgcaccccc ccaccccggc
     4741 gctcgacgat ctcctggaag ccctcgatca ccgcatccgg gcggtcggcg aagacgcggc
     4801 ccctggagga gacctgcccg gatccgggct ttgcgacctg atcgacaccc tgagcgcccg
     4861 gatccggagc atcgatgccg ggtaccggcg gtgccggcac cgcctcgccc tccttccggc
     4921 ggggtgcctc tgcaccggcc cggacggggt gatcctggag gcgaaccggg cggcgggcgc
     4981 gctgctcggt cctgcccccg atcgtctgcg gggtctctcc ttcccggcgc acctccaccc
     5041 cgggtctctt tccgccttcc ggtcggcggt cgccgcgctc gagcggggcg acgaggtccc
     5101 ggtgcaggag ttcctgctcg cacggcccga cggcagcacc ctcccggtcg gggcggccgt
     5161 cgcggtcgtg cgtgacgggg cgatcgagca cctctggatc ctatccgacg catccgggca
     5221 gaagatgatc gaagaggcac gccggaggag cgaagaacgt taccgggaac tgaccgggaa
     5281 cataagcgac gccttcctcg cgctcgacca ggaggaccgg atcatatcat ggaaccgggt
     5341 ggcggccaac ctctcgggcc accccggaga ggaggtcatc gggaagagca tctacgacgt
     5401 ctaccccgaa cttcggactg aagaggtcgt cgggttcttc cgcgaagtcc gggagaccgg
     5461 ccggcccggc atgagcgagt gccggtttca cgtcggcggt cgggaccacg ccttcgaggt
     5521 gcgcgccgtt cccacccgtg aaggcatctc catctacatc cgggacataa cggatcgccg
     5581 caacgccgaa gaagcgctca ggaagagcga agagcgctac cgcgccatcg tcgagaacca
     5641 gaccgagatg atctaccgcc ggctccccga cggcaccatc acctttgcaa acgacgctta
     5701 ctgccgctcc atcggcatcc cgccgggcga cctcgtcggg cggcgctacg ctcccgcgat
     5761 accggccgac gaccaggccc ggatccagaa gagccttcac tccctcaccc ccgctcaccc
     5821 ggtactggcg gtcgagcacc gggtggtcat gccggacggc gagatccggt ggcagcagtg
     5881 gacccacatg gcgttctttg acgagacggg ctccctcgtc gagtaccagt cggtgggccg
     5941 cgacacgagc gctgcccgga tggcggaaga ggcgctccgc cttgcgaaca aaaaactcaa
     6001 cctgctctcg gacgtgaccc ggcacgacac cctgaatcgg ctcaacgtgc tcaccggcta
     6061 catcgacctc tcccgcgaag agacgaaaga cccggatctg caggagtact accggaagga
     6121 gggggagatc gtccgggaga tccggcgcta catggtcttc accggggagt accgggagat
     6181 cggcgtggct gcacccatct ggcaggacgt gggccggatc gctcgcgagg cggcggcagg
     6241 gctcgatatg ggagaggtag ccgtcgaggt gcaggtcgcc gatatcgagg tgtatgccga
     6301 ccccctcatc gtccaggtct ttgcaaacct catcgacaac tcgctccggc acggcggggg
     6361 cgtcgcccgg atacggatct ctgcccgcga gtcagaccgc ggagtcacca tcatctacga
     6421 ggacgacggc atcggtatac cgctcgccga gaaagaggat atattcgaac gggggttcgg
     6481 gctcttcctt gcccgggaga tcctctcgat caccgatctc tctctccggg agaccggccg
     6541 gcccggggag ggtgcgcggt ttgagatcgg cgttccccgg gggtattatc ggttcaccgg
     6601 tcccggccaa gctccgtgag cttcatctcg gccgcgccga ggaggtcctc gcactgcttc
     6661 accaggagcg caccgcgctc gtatatggag atgctctcct cgaggctcgc gtcgccgtcc
     6721 tccagccgcc ggacaatccc gcgcagctcc tcgagcatct cttcaaacgt cttcgtcata
     6781 tgtcacttcc tcgatgacgg ccctgcaccg gccgtccttc atcagcagca tcacccggtc
     6841 gccgggatgg aggtcgcggg cgctccgcat gaccctccgt cccgacccga cgatacagta
     6901 gccccggtcg agggccgcga gcgggtccct ccccagaagc tccgcacggg cttccgcgag
     6961 cgccgcgcgc tcccgctgca cccgggcgag cgccgcgcgc cggagcaggt ccgcgtgctc
     7021 gacgaggcgc tgcatccgct cgtggacccg gcgttcgagc cgccgggggt gcatgcgcgc
     7081 ccgcaggtca tcgacctcgt tttccgctgc ggcaatccgg tgggagagga gcgcccgcat
     7141 cctctcgcgg tgcccggcga cctcccggag caggtccacg cggtcgggca cggcccgctc
     7201 cgccgcagcc gacggcgtcg gcgccgggag gtccgcggcg aagtcgcaga gggcggtatc
     7261 cacctcgtgg ccgacggcgc tgatcaccgg cgtcctgcac gctgcgacgg ccctgacgac
     7321 gtcggggtgg ttgaagggga agaggtcctc aaagctcccg ccgccccgac cgacgatgat
     7381 cacatccacg agcccgtcga tccgccggat cgcctccgcg atatccgcgt gcgccccctc
     7441 cccctggacg gccgccgggg agaggaccac ctcgaccggg tagcgccggg atatgaccga
     7501 gaggatatcc tgtaacgctg cgccggtccg cgaggtgacc acccccaccc tgcgcgggaa
     7561 ggccggcagg ggccgctttc gttccggcga aaagagccct tcggcgtcga gctcccgctt
     7621 ccagcgctcg accatgaggt ggcgctcgcc ggcgcctgcc gggagcagtc ttctgacgat
     7681 cagctgatac ctgccgtgcg gttcgtagac ctccagggtc ccgaaggcaa gcacgcccat
     7741 gccgtttctg ggtgcaaagc ggagctctgc cgcgcaggaa cgccacatga cgcagttgat
     7801 cagcgccgaa ctcctctccc ctccctccga gagcgagaag tagtggtggc cggatgagtg
     7861 gttcttgtag ttggtcacct ccccccgcac ccagatctcc tggaggcggg cgtcgtcaag
     7921 gaggtcgcag atgagggtcg agacctcaga gactccaaag atcaggcccc cgggttgatc
     7981 cggatcggcg gcaggaccgc tcaacatcat cacaatctat tagaccgatg gtttatatca
     8041 agtaatatta tgccccgttt cgccgtctcg accatgttct tccacgagta cccctgcgac
     8101 catatcttcg actacatcgc cgaatcaggc ctcgacagcc tcgagttctg ggtcgagacc
     8161 ccgcatttct ggcttgccgg ccgccccgaa ggcgacctct cctgctgcat cgccgatcac
     8221 ccggacctct cccccatcac catccacgcc ccgtcgctcg acttaaatcc ctgctccatc
     8281 aaccccgagg tcgccgcgat atcggtcgac tacgccgtcg agtccgtccg gatggccgac
     8341 cggatgggcg ccgccgtggt cacggtccac cccgggagac ggacggcgaa acggcccccg
     8401 agcgcctacg actaccagcg gttcgagaag tatgtcgcac ggctccggga ggcggcgtcg
     8461 gaggcgcggg tgaaggtggc gatcgagaac ctggagcccc gggtcaacgc cctgctcacc
     8521 acggcggagg atgcggccga ggtgctcgaa cgagagccct ggctccggtt caccctggat
     8581 atggggcacg ccatgatgac gtcctgtgac gaggtgctcc ggttcatcga cctctgtatc
     8641 gagcggatgg taaacgtcca cgtcagcgga ctcggagaga acgggcggcc ccactacccc
     8701 atccacgacg acccggatgc gatgcgggtt ctcaccgaac ttgccgaccg cggctacgac
     8761 ggatacctga cccttgaact cgaggatatg gtctttcccg gggcactctc gtctgaagag
     8821 aagatcgtgg tccttaaacg ccaattagag accttcgaga aggtcttctc ctgaagcgct
     8881 gggtttataa cccctgcctg caacataatt catatattca gcaggtaacc ggacaaccgg
     8941 atgttgccgc ttggtgcggc gtggagtaaa tggtaatcgt agaccttgtc accgtagaga
     9001 tcatactctt tatcatctgt ctgctcctct cggccttctt ctcaagttct gaggtcgccc
     9061 ttatatcgat aacccgggcg aaagtccgcg cactcttaaa ccagggtcgg aagggagcgg
     9121 aagcgctcga cgtactgaaa cgatcgaccg acaccatcct gatcaccatc ctgatcggga
     9181 acaacatagc aaacgttgcc gcagcatcgc ttgcgaccgc gatcgcgatc ggcatctacg
     9241 gcgacgtcgg tatcggcatc gcgaccggcg tcacggtcat cctgatgctg gtcttcggcg
     9301 agatcgggcc gaagatgtac gcctcccggc acaccgagag cctcgcgctc agcgtcgccc
     9361 ggcccatcct ctacctctca aaggcgctct accccgtgct ctggatatcg gaccggatca
     9421 accggcagtt tgccttcagg cccggcgtga ccgaaccggt cgtcaccgag gaggagatca
     9481 aggagtggat cgacgtcggt gaggaggagg ggacgatcga ggagcaggag cgggatatgc
     9541 tctactccgt cctgcgcttc ggcgacacga cggtccgcga ggtgatgacg ccccggatcg
     9601 acgtcgtgat gatcgaggat gcgaacacga tcgagggcgc cctcgcctta ttcaacgaga
     9661 ccggtttctc ccggatcccc gtctaccacg aacagatcga caacgtcgtc ggtctcctga
     9721 acgtgaaaga cgtctttgcg gcggtcgtcc gccagcagac gagcgcgatg atccgcgacg
     9781 tgatgtacga gccctacttc gtccccgaga gcaagaagat cgacgaactc ttgaaggaac
     9841 tccaggtgaa gaagcagcat atggccgtcg tcctcgacga gtacgggtcg tttgccggca
     9901 tcgtgacggt cgaggatatg cttgaggagc tggtcggcga gatcctggac gagttcgacg
     9961 aggaggagcc cgaggtgcaa cgggtcgagg agaacgtcta cctggtcgac gcacgggcat
    10021 gggtggacca cctcaacgaa gacctgcacc tcgccctccc gctgacggat gcctacgaga
    10081 gcatcggcgg cctcgtcatc gaccgcctgg ggcacatccc ccgccgcggc gaggtggtca
    10141 aactcgagga gagcaacatc acgctggtgg tgatgcagat gcggggccgc cgcctcgtca
    10201 aggtgaaact gatcgtcgct cccccgcctg ctccggagga gtcccggtaa ggagagaatg
    10261 tccatggaga ggaagaaacg gattgcaaca ctcgcatcgg gaagaggatc gaactttcag
    10321 gcggtcatcg atgccgtcgc ggccggggat atcccggcga tctgtgtcgg cctcatcacc
    10381 gacaacccgc aggcctacgc ggtcgagcgg gcaagagccg ccggcatacc ggtgacggtc
    10441 gtcgattatg cgcggtttcc ctcaaaggcc gcttacgagg aggcgctcct tgccgcgatg
    10501 cgaggttgcc gggcggatct cttcgtcctt gccggctaca tgcggatcct cggggccgcg
    10561 atcgtgcatg agttctcggg ccggatgatg aacatccacc ccgccctgct cccggcgttc
    10621 gccggcctgc acgcgcagcg ccaggcgatc gagtacgggg tgaaggtctc gggctgcacc
    10681 gtccacctcg tggacgaggg gatggatacg ggccccatcg tcgtccagcg atgcgtcccg
    10741 gtcctctcgg acgacgacga gtcggcgctt gccgaccgga tcctcgcaga ggagcacaga
    10801 gccctcccgc ttgccgtgaa actcttctgc gaggaccgcc tggaggtcgc cggccgaaag
    10861 gtgcgggtcc gctgacaccg gcctgctata tatcctttag tcgctaaccg taagacccac
    10921 caatcggcag aacggatcgg atcatggcag acaccacacg aagatcgggc tcgcggaggc
    10981 ttgcccgcga gaggattgcc atccttttcg cgcgtgccgc ggagttctac cccgataacc
    11041 ccgcatggag caaccggtgc gtggaactcg cgcgaaagat cggcatgcgc caccgggtgc
    11101 ggatagatcg gccgctgaaa cgccggttct gccgccggtg cagcgtctac ctggtcccgg
    11161 gcgcgaatgc ccgggtccgg atctaccgcg ggcgcgtgat cgtcacctgt cttgcctgcg
    11221 ggcaccggtc acgctaccct gtcgggaggc gaacctcgtg agcagagagt cgtttcagga
    11281 tatcaagccc accgtctggg tggggaaacg gggcatcagc agcctcatga tcgatgagat
    11341 acggcgccag ctcgagaccc ggaagatcgt caaggtccgg tggctcagga acaccgaggt
    11401 cgatcccgag gaggtggcgg tgtcggccgg tgcggttctc atcgaggtca gggggcggac
    11461 cttcgtcctt gcagagcggc gaagtcgatc gcccggtcgc aattctcgaa atatataaaa
    11521 catcactgga cgaatatgta aagactgtta gcaatagtga ggttcatcta tgacgactgt
    11581 atacgacatc cctgccgata tgttgatccg gcaggcagca gaagaactta agaaaaatcc
    11641 cgcaattcag ccccccgact gggccgattt tgtgaagacg ggggtacaca aagagatgcc
    11701 gcccgagaac gacgactggt ggtacgtgcg cgcggcggcg gtcttccggc gcatctacac
    11761 cgacggcccc gtcgggaccc agaggatgcg ctccatctac ggcggcaaac acgacagggg
    11821 ctcggctccg tcccggttca agcggggaag cgggtcgatc gtccggaaga tcttccagca
    11881 gctcgaggcg gccggctacg tcgctcacac aagcgaaggg cgcatggtca ccgcggccgg
    11941 cagatcgttc ctcgataacg ttgcaaacaa cgtaaaatct caagtttccg ggatttcacc
    12001 cggaatcgcg aagtattgat cagggaggta tgactgatgg tagatgacga actcgcagaa
    12061 ctccgccgcc ggaagatgga acagatgcaa cggcaggcga tggatcagca ggcgatggag
    12121 gacgaagtag aacgccagcg gcagatcgag tcacagatcc gcgtcgtgct catggagatc
    12181 ctcgagcctg aagcgaggga gaggctcaat accatcaagt tgacccggcc ggagtttgca
    12241 aaggcggtcg aacagcagct ggtggtgctt gcccagagcg gcagggtccg gcagcggatc
    12301 accgacgatc agttgaaagc cctgctgacg cagttgacgc cgtcaaagaa agagttcagg
    12361 attacgcgaa agtaatggaa gcaggtctgc tcttctcggg agggaaagac agcgcgcttg
    12421 cagcagtcat gctcgcgcgc gactacggcg tggaactgaa tacctgcgta ttcgatcccg
    12481 ctcgcgatgt tccggcggtg cgggccgcag cggccgcatt acacctcccc ttcaggcgga
    12541 gggtgcttgg aagagacctc ctcgaggaag ccgccgatat gcttcttgtc tgcggttacc
    12601 caaacgacgc gatcaccatg gtccatcggg cggcaatcga ggcccttgcg ggtgagtatc
    12661 gggttgtcgg cgacggcacc cggcgcgaag accgggttcc ccggctcgag cggtccgcgg
    12721 tgcagcacct ggagatgacc accggcagtt cctacgtccg gccgctcctc ggctacggga
    12781 aaccggaggt ggaacgcctc gcgggaaggc tgcttgtggt ccggtacggg gagacgggca
    12841 gtatagggaa cggcgattac gagcaggaga tccgggaggc catccgtgcc cgcggcatcg
    12901 atccggcgcc gttcttcccg gccggccacc tgcagtcgct cgtggtcggg atgcgggcga
    12961 cctgaagaag gatgcaggag aaagagtaac tatgagcaag ttgatgaaag gccggaagat
    13021 ccggctggca aaggcatgcg agcagaaccg ccgcgtgccg gcatgggtga tgatcaggac
    13081 caaccgtgcg gtttcgtcgc atccgaagcg gcgcaactgg agacggagca gcttgaaggt
    13141 gtaaggttat ggcagaggcg ttaaaagagc acatctatat catccctctc cgcgaggtga
    13201 agcgttcacc ccggtggaag cggggcaaca ccgccatcaa ggatatccgg gcgtttctcg
    13261 aacggcacat gaagagtgcg gacgtcaaac tggaccggag catcaacgag aaggtctggg
    13321 agaacgggag tggaaagccc ccgcgaaaga ttcgcgtccg cgcgatgaag ttcgaggacg
    13381 ggcaggtcca ggccgagctc gccgaggagt gagatgac
//
LOCUS       DAOSEE010000053        13098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1846_length_13098_cov_238.736410, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000053 DAOSEE010000000
VERSION     DAOSEE010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 13098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13098
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1846_length_13098_cov_238.736410"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            287..505
                     /locus_tag="PLI76_05565"
     CDS             287..505
                     /locus_tag="PLI76_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOI61533.1"
                     /translation="MVLEYINAALDHARYEIIEDAEPYYGEVPELPGVWATGRTLEEC
                     RRNLAGVIDEWLILLLRRGLPIPPVAGT"
     gene            complement(605..1774)
                     /locus_tag="PLI76_05570"
     CDS             complement(605..1774)
                     /locus_tag="PLI76_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOI61534.1"
                     /translation="MNPTYITSLDKVAGIGAQERAALAPVLENFAFRSNDYYLSLIDW
                     EDTKDPIRRLIIPSPDELEPWGRLDPSSEHQYTRAPGLQHKYRETALLLVSDLCGGFC
                     RYCFRKRLFIEDAREVNKDVSGGLAYIRDHPEITNVLLTGGDPLVLGTDRLRDILNHL
                     RAIRHVRIIRIGTKMLAYDPYRIINDPALVDAVRGCYDGENLVYIMAQFNHPRELTDA
                     ACRAIALLQDAGAVIMNQTPLIRGINDDPAVLSKLLGRLSFVGVNPYYVFQCRPTTGN
                     RTFAVPVEESYRIFEQARATCSGLSKQARFVMSHATGKIEVVGKTGGFTYFKYNQAAD
                     PDDIGRFMVYKSNPQAYWFDDYTDLVDEGRLGEPEEAVGDARDLALSPGAGYTLG"
     gene            complement(2042..2416)
                     /locus_tag="PLI76_05575"
     CDS             complement(2042..2416)
                     /locus_tag="PLI76_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing protein"
                     /protein_id="HOI61535.1"
                     /translation="MPHKCTQCGREFEDGSTKILKGCPSCGGKKFLYIREAERHDDVL
                     KEKTIEEIARETGEDVLEVREDKRRAEIEVFERIESIRILGPGSYELNLERLARSDEV
                     VVGLEKEGRYAVDIISMAKKKK"
     gene            complement(2432..2800)
                     /locus_tag="PLI76_05580"
     CDS             complement(2432..2800)
                     /locus_tag="PLI76_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2073 domain-containing protein"
                     /protein_id="HOI61536.1"
                     /translation="MIQGVQIDLISAERLDRLTTMEKIRLILDDVMEGNIVVLEKGLA
                     PDEQSKLIEITMREIAPDGFSGIEMETYPVRDAPTGFLAKLLGGKRTEARLTVIGPAN
                     QLKTLRKEKDLISAWVSSSR"
     gene            complement(2806..3447)
                     /locus_tag="PLI76_05585"
     CDS             complement(2806..3447)
                     /locus_tag="PLI76_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Era-like GTP-binding protein"
                     /protein_id="HOI61537.1"
                     /translation="MTFLVRTKQKISRFLKTLFKRRTCRIGIYGPPNAGKTTLANRIV
                     RDWTGDAVGPVSEVPHETRRVRRKEDITISSQNGNSVTIDIVDTPGVTTKIDYNEFVE
                     YGIEKEEAVKRAREATEGVAEAMHWLREDIDGVIYMLDATQDPFQQVNIMLVGIIESR
                     KLPVLIVANKNDLPDASPARIRSAFPQHPVISISSLEGNNVNELYEKMTSYFG"
     gene            3659..4222
                     /locus_tag="PLI76_05590"
     CDS             3659..4222
                     /locus_tag="PLI76_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOI61538.1"
                     /translation="MMNNSDVIHFETRVPIREVMRSHPTTIDVGETVARAAQAMCRDE
                     VGSCIVLQNSLPIGIVTEEDINCKVVAKDMKPGNVHVDRIMSTPLITIGADRMVGDAV
                     AMMVKHRVRRLPVVENQRVIGIVTVRDILTVAAEVNELLADLIEINREEVYTMGVCDR
                     CGDMSDDLSRVDNLMLCHACREEEQLL"
     gene            4219..4974
                     /locus_tag="PLI76_05595"
     CDS             4219..4974
                     /locus_tag="PLI76_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOI61539.1"
                     /translation="MKVAENLMVDIPTLCNGDYITKARSILRDDVFRELYVLDEKNRL
                     AGYLDISDVLRVVDTKSNVTIEGFVREAARVAPDTPLTGVGRAIMNARTNSAAVVDED
                     GVFLGGVLFSELFPILISRRDICGRVKDAMSPEPVTCAPEDPIHRIYNLIIASGFIAF
                     PVVQKREPIGIVSRRDLLRAGSIRMSVKNQADTTVDRVMTTPVITVTPDDPVATAAHL
                     MVDHDVSILPVIDERKRIVGVIDRHDVSSCLAP"
     gene            5019..5966
                     /locus_tag="PLI76_05600"
     CDS             5019..5966
                     /locus_tag="PLI76_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOI61540.1"
                     /translation="MQPNSNNAEKKPADRLLKSPGKRDRGPVNFKTKIAGYEGEIMAI
                     ATKNVVTAQRTTTIIQAVEIMTREGFRRLPLIDPGTHRLRGIVTVSDIIDFMGGGDKF
                     NLVQVKHGGNFLAAVNEELREIMTSQPVAMPVTGSIADAVDIIINRNVGGLPITDAEG
                     TLKGIVTERDVMKVLATEHSGRKVEDVMRSSVRVTSPDTPIGSVCREMVTCRFRRLPV
                     VVDDVLCGIVTATDIMSYLGKGRAFEQLTTGDSAEVMGAPVRSLLSGELYTTTPDRNI
                     HEIAVDMIKKRVGAFPVIEDSHLVGLVTEYDLVKAFAEG"
     gene            5975..6814
                     /locus_tag="PLI76_05605"
     CDS             5975..6814
                     /locus_tag="PLI76_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOI61541.1"
                     /translation="MRAVDVMSAPVYIVAPADNVAYARNLMLRYRVSRLPVMEEDELR
                     GILTKKDIAYRLRQTEPMWRRRPIDRIPVSILMAENPITVAPDTSIHDIAAMMLDRDI
                     SGLPVVDEGRVVGIVTKLDIMRSAHVRGLFARVDEVMEDAVTVSRYHSLDHVIDTIKG
                     KNDKLIVVNDNGSLAGIITESNLAFYEYLDGRMNLPRKDVTHLRKEEPGGQKRFRYVV
                     EVSAVAEDIMSRPVVTVSPGVSIQDAVGLMLEHQINSLVVVENGDIRGVLKRDDIIKE
                     VAK"
     gene            6811..7593
                     /locus_tag="PLI76_05610"
     CDS             6811..7593
                     /locus_tag="PLI76_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOI61542.1"
                     /translation="MSDGFQILVKDVMAKPITIAKSAFVSEALEKMLDEGVDPLIVTN
                     NGTVIGTTSRAAIAETLGSRKTQALKATSIHVANTVEENFTSAYPDQNIDILVPLLQH
                     YKLVVVFDAEHRLIGQVTAGDLLKVLRPPGNLPDVMEPAHTIQVEERAVHLRRRMLDG
                     GINRFIVEEGNQTLGIVTETDVAKALYALKDIVEGTRQEFRIRNLLVRDIMSTPLISV
                     NADTDVSDVIDLMLKKNIGSVPITQNGRIAGVVTRNSLVQAL"
     gene            7738..10752
                     /locus_tag="PLI76_05615"
     CDS             7738..10752
                     /locus_tag="PLI76_05615"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOI61543.1"
                     /translation="MIILHSFWADGSPGAFHVWGEDPALPRKGMPRRGRKPKKPPVLP
                     HPFAADHAALTAALDLAGEPVTVTVLLPATGQDPLPSPECEPGSVVPDRADCVTYRVP
                     AIRLPGTVPVACLADLPSGPGYGATIRFWSEAARFALGLVVRQSFVPGARGWEALIRG
                     EDRDRVARLAGALPPACRFWAAGADGSPPDPAALVTSFLDRAVHTLVTGALRERPLLQ
                     KPRGRPRKIVPFGEQWVEVLSGSRDDLAGDAEEVARFYEQLDDWLSPAIAPTPLRACF
                     RLEEPEEESGQWHLTFHLQAADDPGIVIPAGDIWKKRGDVRTSLAQRFEDPQDRLLTD
                     LGRASRIYPVLQSSLTSPRPAGVNLDTSEAYAFLSEWTPLLMDSGFGVILPAWWKSAA
                     SRPSLHLTAKPAREEEGRLGLATLVSYDWTVALGDKAISPEEFEELASLKIPLVRMHG
                     QWRSIDRDEIKKALKAFTRRYPGGTMTAGELLRIAAGADPGGPAVSDVAAEGWLRGFL
                     DRSGEGGTLEAVPQPAAFSGTLRPYQQRGLSWLSFLARNGCGACLADDMGLGKTVQVI
                     AYLLAEEERGTPATPALLICPMSIVGNWRREFARFAPGLTVAVHHGSARACGDAFARQ
                     AAGSDVTITTYPLAARDRDLLASVDWNCIVLDEAQNIKNPSTKQAQAVRSFKAPQRIA
                     LTGTPVENRLTELWSIMEFLNPGYLGSFESFHSGFAVPIERYRDPAKTGHLRELIRPF
                     VLRRMKTDRSIIGDLPEKMEMKVFCSLTAEQATLYEAVVQEMLERADEAEGIRRRGIV
                     LGGLTRLKQICDHPALFLHDTAAFEGRSGKIARLLEMLEEVLDAGERALIFTQFAEFG
                     KILEPYIRERFGTAVLYLHGGTPRQKRDEMVQRFQQPGGPPIFLLSLKAGGTGLNLTA
                     ANHVFHLDRWWNPAVENQATDRAFRIGQTRDVQVRLMIASGTLEEQIDTLIEDKKALA
                     DQIVGKGEDWIGSLSTEQLRDLLTLRRDAVED"
     gene            10756..11700
                     /locus_tag="PLI76_05620"
     CDS             10756..11700
                     /locus_tag="PLI76_05620"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HOI61544.1"
                     /translation="MGSWYYYPSYPPSAPRPVANGLKARSKRGAIGEQWWSQQFIKAL
                     EQMGNASRLQRGKRYARKGQVIGMEVGDGEVRAKVQGSAARPYGVTIRLRALTDAEWG
                     RVFDLMSAQAIFAAQLLMGVVPHDIEKIFSEAGVPLFPTSEKDITTNCTCPDWENPCK
                     HIAAVYYLLAERFDEDPFLLFALRGQSRDEVMAALRERRTEDPEPVTPLAGSFWQGDD
                     TLAGFGIEITGSAPDAGAALKIFGEPPFTVRGNDLSTVLAPAYQAAQEYARAIAGIPG
                     EEPADEQPPGKRADDDLRSPGPAPTVPTPKTGSSRRRR"
     gene            11889..12866
                     /locus_tag="PLI76_05625"
     CDS             11889..12866
                     /locus_tag="PLI76_05625"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HOI61545.1"
                     /translation="MKPTQPVKPDRDVTTLLQAMSRTGFQGRKLGESLGVWTRMVEDP
                     DCTIFMGLSGAMIPAGMQNVLVELVRHRYVDVLVSTGANIFHDTCEHLGVRHYIGHHH
                     ADDAALFEEGIDRIYDVFAYEEEFRSVDAEIARFADEIAPFRGSSREFIRRLGGWLCT
                     RRPEGRSLIATCAEYDVPIFIPALGDSSIGIGLVMARRRGVDVDVDQLADTDEITRMV
                     EESQKTGVIYVGGGVPKNFIQQTQVIASIHEQHLGGHAYAVQYTTDAPHWGGLSGCTF
                     EEAISWGKEAPESPRVQCFCDATIALPLVASALIGSGVERARRSPRREA"
BASE COUNT         2569 a         4232 c         3975 g         2322 t
ORIGIN      
        1 agtttcagga acccatctac cgaactttgc atcttcacgc gagctacctg gtgcggatag
       61 cggtacagcc tcccgcgctg gtgcgaaagg ggggtcgagg gcgtggtcga ggatcagggc
      121 atacacagcc tcctgcgctg gtgcgaaagg ggtctgtgaa gcgtggagaa tccatgactg
      181 cagacaggtc ggcgaggctg caacgatcca tatcatgagg caaagttgcc tccggcccca
      241 accggcaaac gaaaacccta tggttggaac cctacagatg cgtagtatgg tccttgagta
      301 catcaacgcg gcactcgacc acgcccgcta cgagatcatc gaggatgccg aaccatacta
      361 cggagaggtt ccggaactcc ccggcgtatg ggcaaccggg aggacactcg aagagtgcag
      421 gagaaacctt gccggcgtca tcgacgaatg gctgatcctc ttgctccggc gcggacttcc
      481 gataccgccg gttgccggaa cataaacctc agacctctcc tccataaagc cggcgcacct
      541 gacttcgcag gagatgccgg gagaccggca ccctgccggc gagaaaccat ccccgggacc
      601 cccctcaccc cagcgtgtac cccgcgcccg gcgacagcgc gagatcccgg gcatcgccca
      661 ccgcctcctc aggctccccg agcctcccct catccacgag gtccgtgtag tcgtcgaacc
      721 agtaggcctg cgggttgctc ttatagacca taaaccgccc gatatcgtcc ggatccgccg
      781 cctggttgta cttgaagtac gtgaaccccc cggtcttccc caccacctcg atctttcccg
      841 tcgcatggga catgacaaac cgcgcctgct tcgagagccc cgaacaggtc gcccgcgcct
      901 gctcgaatat acggtaagac tcctccaccg gcaccgcaaa cgtccggttc ccggtcgtcg
      961 gccggcactg gaagacgtag taggggttga ccccgacgaa cgagagccgc ccgagaagct
     1021 tcgacagcac cgccgggtcg tcgttgatac cgcggatcag gggcgtctgg ttcatgatga
     1081 ccgctcccgc atcctgcagg agcgctatcg cccggcacgc cgcatcggtc agctcgcgcg
     1141 ggtggttgaa ctgggccatg atgtagacca ggttctcccc gtcgtagcac ccccggacgg
     1201 catccacgag ggccggatcg ttgatgatcc ggtacggatc gtaggccagc atcttcgtcc
     1261 ctatccgtat gatccggacg tgccggatcg cccggaggtg gttgagtata tcgcgcagcc
     1321 ggtcggtccc gaggaccaga gggtcgcccc ccgtcaggag gacgttcgtg atctcggggt
     1381 ggtcgcggat gtaggcaagc ccccccgaga cgtctttgtt cacctcgcgc gcatcttcga
     1441 tgaagaggcg cttgcggaaa cagtaacggc agaacccgcc gcagaggtcg ctcacgagga
     1501 gcagagccgt ctcccggtac ttatgctgca gccccggcgc ccgggtgtac tggtgctccg
     1561 acgacgggtc gaggcgcccc cagggctcaa gctcgtccgg cgagggtatg atcaggcgcc
     1621 ggatgggatc ctttgtgtcc tcccagtcga tcagcgagag gtagtagtca ttcgagcgga
     1681 acgcaaagtt ctccagcacc ggcgcaagag cggcacgctc ctgtgcaccg atgccggcga
     1741 ccttatccag tgatgtgata tatgtcggat tcatactctt ccttcatgtc ggcatccccc
     1801 gctgtgcggt gcaaccgggg atatacctca tgagagcatg aatgacggca gtcaggtacg
     1861 gtaaagggtt catgagagtg ccgtactccc atgcaggaaa ccatcgcccg ggtatgcgcc
     1921 agagggtgcc gggttatacc agatatcaaa aaagggcact tgagatatat aatagttttg
     1981 ggaagggggc atcatcgcgc gaaaaccgcc ctgttttcgc gagaaggcgc gtaaataccg
     2041 attatttctt cttcttcgcc atggagatga tatccaccgc gtatcgcccc tccttctcca
     2101 ggccgacgac gacctcatcg gaccgggcca gtctctcgag gttcagctcg tacgaacccg
     2161 gaccgagtat gcggatactc tcgatccgct caaagacctc gatctccgcc cttcgcttat
     2221 cctcgcggac ctcaagcaca tcttcgcccg tctcccgggc tatctcctcg atcgtcttct
     2281 ctttcagcac gtcgtcgtgc cgctcggcct cacggatata gagaaacttc ttcccgccgc
     2341 agcttgggca tcccttcagt atcttcgtcg aaccgtcttc gaactccctg ccgcattgcg
     2401 tgcacttgtg aggcataccc cctcacccca cctacctcga ggaggatacc catgcgctga
     2461 tgagatcctt ctccttcctg agcgtcttga gctggtttgc gggcccgatc acggtcagcc
     2521 gggcctccgt tcgcttccct ccgagaagtt tggcaagaaa tccggtcggt gcatccctga
     2581 cgggataggt ctccatctcg atccccgaga acccgtccgg cgcaatctcc cgcatcgtga
     2641 tctcgatgag cttgctctgc tcgtccggcg caaggccctt ctccagaacg acgatgttcc
     2701 cctccatgac atcgtcaagg atcaggcgaa tcttctccat cgtcgtcagc cggtcaaggc
     2761 gctccgccga gataagatct atctgtacac cctgtatcat ctccatcacc caaagtacga
     2821 tgtcatcttc tcgtacagct cgttcacgtt gttcccctca aggctcgata tcgatatcac
     2881 ggggtgctga ggaaacgcac tcctgatccg cgcgggggaa gcatcgggaa gatcgttctt
     2941 gttcgcgacg atcaggaccg ggagcttgcg gctctcgatg ataccgacga gcatgatgtt
     3001 cacctgctgg aacggatcct gcgtggcatc gagcatgtag atgacgccgt cgatatcctc
     3061 gcggagccag tgcatcgcct cggccacgcc ttccgttgcc tccctggccc gcttgaccgc
     3121 ctcctccttc tcgataccgt actcgacgaa ctcgttatag tcgatcttgg tcgtcacgcc
     3181 cggcgtatcg acgatatcga tcgtgaccga gttgccgttc tggctcgata tggtgatatc
     3241 ctctttgcgc cgcacacggc gggtctcgtg aggcacctcg ctgaccggcc cgactgcatc
     3301 tcctgtccaa tcccgcacaa tcctgtttgc aagcgtcgtc ttgcctgcat tgggaggccc
     3361 gtagatcccg atgcggcagg tccgcctttt aaagagcgtc ttgaggaacc gcgagatctt
     3421 ctgttttgtt cgaactaaga acgtcatttg gcaccctccg ataggccgat tcacctacac
     3481 ctgggtaaac atgacacccc cgaataaata taattatatg ttccatccgg cgtaatcccc
     3541 tcaaatcggg gataaacggt aatcgttccc gccgagagaa ttcttttata ctgcaaaaac
     3601 ctagatacac ttgcacctgg agaatcggct ctcaccgcga ccagattagg agaatcccat
     3661 aatgaacaat agcgacgtca tccactttga gacacgggta ccaatcaggg aggtcatgcg
     3721 gagccatcca acgaccatcg atgttgggga gacggtcgcc cgtgcggcac aggccatgtg
     3781 ccgcgacgag gtcggcagtt gtatcgtact gcaaaacagc ctgcctatcg ggatcgtcac
     3841 ggaggaggat atcaactgta aagtcgttgc aaaggatatg aagcccggca acgttcacgt
     3901 cgaccggatc atgagcaccc cgctgatcac gatcggcgcc gataggatgg tcggggacgc
     3961 cgtggccatg atggtgaagc accgcgtccg ccggctcccc gtcgtcgaga accagagggt
     4021 catcggtatc gtcacggtca gggatatcct caccgtcgcg gccgaggtga acgaactgct
     4081 ggcggatctc atcgagatca accgcgaaga ggtctacacc atgggcgtct gcgaccggtg
     4141 cggggatatg tccgatgacc tctcgcgggt cgacaacctt atgctctgcc acgcctgtcg
     4201 ggaggaggaa cagttgctat gaaagtggcc gagaacctga tggtggatat acccaccctg
     4261 tgcaatggcg actacatcac gaaagcacgg agcatccttc gggacgacgt ctttcgcgaa
     4321 ctctacgtcc tggacgagaa gaaccggctg gcaggctacc tcgacatatc cgacgtcctg
     4381 cgcgtcgtgg atacgaagtc caacgtcacc atcgaagggt ttgtcaggga agccgcccgg
     4441 gtcgccccgg atacgcctct caccggggtc ggccgcgcca tcatgaacgc ccgcacgaac
     4501 agcgccgcag tcgtcgatga agacggcgtc ttcctgggcg gcgtgctctt ctcggaactc
     4561 ttccccatcc tgatctcccg ccgcgatatc tgcggcaggg tcaaagacgc catgtcgccc
     4621 gaacccgtca cctgcgcacc cgaggacccc atccaccgca tctataacct gatcatcgca
     4681 agcggcttca tcgcgtttcc cgtggtccag aagagggaac ccatcggcat cgtatcccgc
     4741 cgggatctcc tgcgcgcggg aagcatccgc atgtcggtga agaaccaggc cgacaccacc
     4801 gtggaccggg tgatgacgac ccccgtcatc acggtgacgc ctgacgatcc ggtcgcgacg
     4861 gcggcgcacc tgatggtcga ccacgacgtc agcatccttc cggtcatcga cgagaggaag
     4921 aggatcgtcg gtgtcatcga ccgccacgac gtctcaagtt gcctcgcccc gtgaggggcg
     4981 ccacccctga atactattat cgccagagga gtgataccat gcaaccgaac tcgaataacg
     5041 cagagaagaa accggccgat cggctcctga aatcgccggg caaacgtgat cgcgggccag
     5101 tcaacttcaa aacaaagatc gccggctacg aaggcgagat catggcgatc gccacgaaga
     5161 acgtcgtcac ggcgcaacgg accaccacga tcatccaggc ggtcgagatc atgacccggg
     5221 aaggatttcg ccggctgccg ctcatcgatc ccgggacaca ccggttgcgg gggatcgtga
     5281 ccgtcagcga catcatcgac ttcatgggcg gcggcgacaa gttcaacctc gtacaggtga
     5341 agcacggcgg caatttcctt gccgccgtca acgaggaact ccgcgagatc atgacctcgc
     5401 agccggtcgc catgccggtt accgggagca tcgccgatgc ggtcgatatc atcatcaacc
     5461 gcaacgtcgg cgggctcccc atcaccgatg ccgaaggaac cctgaagggc atcgtgaccg
     5521 agcgcgacgt gatgaaggtg ctcgccaccg agcactcggg ccgcaaggtc gaggacgtca
     5581 tgcgatcgtc ggtccgcgtc acgagccccg acacaccgat cggcagcgtc tgcagggaga
     5641 tggtgacatg caggttccgg cggctccccg tcgtcgtgga cgacgtcctc tgcggcatcg
     5701 tcaccgccac cgatatcatg agttacctcg ggaagggcag ggcctttgaa cagctgacga
     5761 ccggggattc cgccgaggtg atgggggcgc cggtgcgttc gctcctctcc ggggaactct
     5821 acaccaccac gccggaccga aacatccacg agatcgccgt cgatatgatc aaaaaacgcg
     5881 tcggggcgtt ccccgtcata gaggactccc acctcgtcgg cctcgtgacg gagtacgatc
     5941 tcgtgaaagc atttgcagaa gggtgattga atatatgcgc gccgtagatg tgatgtctgc
     6001 cccggtgtac atcgttgcac cggcggataa cgtggcctat gcccggaacc tcatgctcag
     6061 gtaccgcgta tcgaggctgc ctgtgatgga ggaggatgaa ctccggggca tcctcaccaa
     6121 gaaggatatc gcctaccgtc tcaggcagac ggaaccgatg tggcgccggc gcccgatcga
     6181 ccggatcccg gtcagcatcc tgatggcgga gaacccgatc accgtagcgc ccgataccag
     6241 catccacgat atcgcggcga tgatgctcga ccgcgacata tcggggctgc cggtcgtcga
     6301 cgaaggcagg gtggtcggga tcgtcaccaa actggacatc atgcggtcgg cccatgtgcg
     6361 cggtctcttc gcccgggtcg acgaggtcat ggaggatgca gtcaccgtca gccggtatca
     6421 ctcgcttgac cacgtcatcg acacgataaa gggaaagaac gataaactta tcgtcgttaa
     6481 cgacaatgga agccttgcag gcattataac ggagagtaac ctcgcattct acgagtacct
     6541 ggacggaagg atgaacctgc cgagaaaaga cgtcacccat ctccgcaaag aggagccggg
     6601 aggacagaag cggttcaggt acgttgtcga ggtttcggca gtcgcagagg acatcatgag
     6661 ccgcccggtc gtcaccgtct cccccggcgt atccatccag gatgccgtcg ggctgatgct
     6721 cgagcaccag atcaacagcc tcgtggtcgt ggagaacggc gatatccggg gcgtgctcaa
     6781 gagagatgac atcatcaagg aagtggcaaa atgagcgacg gatttcagat actcgtcaaa
     6841 gacgtgatgg caaaaccgat caccatcgca aaatctgcct ttgtcagcga agcgcttgaa
     6901 aagatgctcg atgagggtgt cgaccccctc atcgtgacca acaacggcac ggtcatcggc
     6961 acgacgtccc gggcggctat cgccgagacg ctcgggagca ggaagaccca ggcgctgaag
     7021 gccacatcca tccacgttgc gaacacggtc gaggagaact tcacctccgc gtatccggac
     7081 cagaacatcg atatcctggt gccgctgctc cagcactaca aactggtcgt ggtcttcgat
     7141 gccgaacacc gcctcatcgg ccaggtgacg gcaggcgacc tcctgaaggt gctccggccc
     7201 ccgggcaacc tccccgacgt catggaaccg gcgcacacca tccaggtcga ggagcgcgcc
     7261 gtccacctcc ggcggaggat gcttgatggc gggatcaacc ggttcatcgt cgaagagggc
     7321 aaccagaccc tcggcatcgt cacggagacc gacgtagcaa aagcgctcta cgcgctcaag
     7381 gatatcgtgg aggggacccg ccaggagttc cggatcagga acctcctcgt gcgcgacatc
     7441 atgagcaccc ccctgatatc ggtgaacgcg gataccgacg tctccgacgt catcgacctg
     7501 atgctcaaga agaacatcgg ttccgtcccg atcacgcaga acggcaggat cgcaggcgtc
     7561 gtaacccgaa actcgctcgt ccaggccctc tgaggagagg acgggtaaac cccatttttg
     7621 gatccgggca cgcccgggcg ttggcccccg ggatgccgat cccacgcgcc gccggcctca
     7681 tcgctccacc gatgcactga tgagggtaca aaaaagagta cataatcaca cgatcgtatg
     7741 atcatcctcc acagtttctg ggccgacgga tctcccgggg cgtttcacgt ctggggggaa
     7801 gatccggccc tgccccggaa agggatgcct cggcggggca ggaagcccaa aaagccgcct
     7861 gttctcccgc acccgttcgc ggccgatcat gccgcgctta ccgcggcgct cgaccttgcc
     7921 ggcgaacccg tgacggtcac cgttctgctc ccggcaaccg ggcaggatcc tcttccttcg
     7981 ccggagtgcg aaccgggatc cgtcgtgccg gaccgggccg actgcgttac ctaccgggtt
     8041 cccgccatcc gcctgcccgg gacggtgccc gtcgcctgcc ttgcggatct cccttccggg
     8101 cccggctacg gggccacgat ccggttctgg tcggaggcgg cacggtttgc cctcggactc
     8161 gtcgtgcggc agtcgttcgt gccgggcgcc cgcggatggg aagccctgat ccgcggggaa
     8221 gaccgtgatc gcgtggcccg cctcgccggg gcgctcccgc cggcctgccg gttctgggcc
     8281 gcaggggcgg atgggtcccc gcccgacccg gcggctctgg tgacgtcgtt tctcgaccgt
     8341 gcggtccaca cgctcgtcac cggcgccctc agggagcggc cgctgctgca gaaaccgcgg
     8401 ggccggccga gaaagatcgt cccgttcggc gaacagtggg tggaggtgct ctccggcagc
     8461 cgggacgacc ttgccggcga cgcggaggag gttgcacggt tttacgagca gcttgacgac
     8521 tggctctcgc cggcgatcgc ccccacccct ctccgcgcct gtttccgcct ggaagagccc
     8581 gaagaggaga gcgggcaatg gcatctgacg ttccacctcc aggccgccga cgaccccggc
     8641 atcgtcatac cggccggcga tatatggaag aagcgcggag atgtgcgcac atctcttgcg
     8701 cagcggtttg aagatccgca ggaccggctc ctcaccgatc tcggccgcgc ctcccgcatc
     8761 taccccgtgc tccagtcaag cctcacgtcc ccgcgcccgg cgggggtgaa cctcgatacg
     8821 tcggaggcct acgcgttcct cagtgaatgg acccctctcc tcatggacag cggctttggc
     8881 gtcatacttc ccgcgtggtg gaagagcgcg gcctcccggc cttccctcca cctcacggcc
     8941 aaacctgccc gggaggagga gggaaggctc ggtcttgcca cgctcgtctc ctacgactgg
     9001 acggtcgccc tcggcgataa agcgatatcg ccggaagagt tcgaggagct cgcgtccctg
     9061 aagatccccc tggtgcggat gcacggccag tggcgctcga tcgaccgaga cgagatcaag
     9121 aaggcgctca aggctttcac gaggaggtat cccggaggca ccatgacggc cggcgaactc
     9181 ctccgcatcg ccgcgggagc agacccgggc ggcccggcgg tgagcgatgt cgcggcggag
     9241 ggatggctcc gcgggttcct tgaccggtcc ggggaaggag gaacgctcga ggccgtcccg
     9301 caaccggcgg cgttctcggg cactctccgg ccgtaccagc agcgcggtct ctcgtggctc
     9361 tcgtttcttg cgcgcaacgg gtgcggcgcc tgccttgccg acgatatggg gcttggaaag
     9421 accgtgcagg tgatcgccta cctcctcgcc gaagaggagc ggggcacccc ggcgacaccc
     9481 gcgctcctca tctgccccat gtcgatcgtc ggaaactggc ggcgcgagtt tgcccgcttc
     9541 gcgccgggcc tcacggtcgc cgtccaccac ggcagcgccc gggcatgcgg cgatgcgttt
     9601 gcccggcagg ccgccggttc cgacgtcacc atcaccacct acccgcttgc tgcgcgcgat
     9661 agagacctcc ttgcatccgt ggactggaac tgcatcgtac tcgacgaggc ccagaacata
     9721 aagaacccct ccacgaaaca ggcgcaggcg gtccggtctt ttaaagcgcc gcaacggatc
     9781 gcgctgaccg ggacgccggt ggagaaccgc ctgaccgagc tctggtcgat catggagttc
     9841 ctcaaccccg ggtacctcgg ttcgtttgaa tcgttccatt ccgggtttgc cgtaccgatc
     9901 gagcggtacc gcgacccggc aaagaccggc cacctccggg agctgatccg gccgttcgtg
     9961 ctccgccgca tgaagaccga ccgctcgatc atcggcgacc tccccgagaa gatggagatg
    10021 aaggtcttct gcagcctcac ggcagaacag gcgacgctct acgaggcggt cgtgcaggag
    10081 atgctcgagc gtgccgacga ggcggagggc atccggcgca ggggaatcgt cctcgggggg
    10141 ctcacccgct taaagcagat atgcgaccac ccggcgcttt ttctgcacga caccgccgcc
    10201 ttcgagggaa ggtccggaaa gatcgcgcgc ctcctggaga tgctcgaaga ggtgctggat
    10261 gcgggcgaac gggccctcat cttcacgcag tttgcggagt tcgggaagat cctcgagccc
    10321 tacatccgcg agcggttcgg cacggccgtc ctctacctgc acggcgggac gccccggcag
    10381 aagcgcgacg agatggtcca gcgtttccag cagccgggag ggccgcctat cttcctcctc
    10441 tctctgaagg ccggggggac ggggctgaac ctgacggcgg ccaaccacgt cttccacctg
    10501 gaccggtggt ggaacccggc cgtcgagaac caggcgaccg atcgggcctt ccggatcggc
    10561 cagacccgcg acgtccaggt gcggctcatg atcgcctcgg gaacgcttga agagcagata
    10621 gacacgctga tcgaggataa gaaggcgctt gccgaccaga tcgtcggcaa aggggaggac
    10681 tggatcggat cgctctccac cgagcagctc cgcgacctcc tgaccctgcg ccgggatgcg
    10741 gtggaggact gacgaatggg ctcatggtat tactacccct cgtatccgcc gtccgccccg
    10801 cggccggtgg ccaacggcct caaggcgcgg agcaagcgcg gggcgatcgg ggagcagtgg
    10861 tggtcgcagc agttcatcaa ggcgctcgag cagatgggga acgcttcacg tctgcagcgc
    10921 gggaagcggt atgcccggaa agggcaggtg atcggtatgg aggtcgggga cggagaggtg
    10981 cgggcgaagg tccagggctc cgccgcgagg ccctacggcg tcaccatccg cctccgcgcc
    11041 ctcaccgacg ccgagtgggg ccgggttttc gacctgatga gtgcgcaggc gatctttgcg
    11101 gcgcagctcc tgatgggtgt ggtgccccac gatatcgaga agatcttttc tgaggcagga
    11161 gttcccctct ttcccacctc cgagaaggat atcacgacga actgcacctg cccggactgg
    11221 gagaacccct gcaaacacat cgcggcggtc tattacctcc tcgcagaacg gtttgacgag
    11281 gaccccttcc tcctgttcgc cctccggggg caaagcaggg atgaggtcat ggcggcactc
    11341 agggagcggc ggacggagga tccggagccg gtcacgcctt tggccggcag tttctggcag
    11401 ggagacgaca cgcttgccgg gttcggcatc gagataaccg ggagcgcccc ggatgccgga
    11461 gccgccctga agatcttcgg cgaacccccg ttcacggtcc ggggcaatga cctctccacc
    11521 gtccttgcac cggcctatca ggcggcgcag gagtatgcac gggcgatcgc cggcatcccc
    11581 ggcgaagaac cggcggatga acagccgccc gggaagcggg cggacgacga tcttcgttct
    11641 cccgggccgg caccaaccgt tccgaccccg aagaccggtt cgtcccgacg ccggcgatag
    11701 gcacgtatgc actcgaagtc catcataccg gtatcgaaca cccctgccac ggcggtggag
    11761 ggggccatgt ccctcagccc tccggaaaac ccgcgttcta aggagaacgg caggctcttt
    11821 gcggtgcacc gtcgcccccg tgcaggcatt taatatcctc cggccgaaac ccataaccag
    11881 aattgaccat gaaaccaacg cagccagtca aaccggatag ggatgtaacg accctccttc
    11941 aagcaatgag caggaccggt tttcagggaa gaaaactcgg agagtcgctc ggtgtctgga
    12001 ccaggatggt cgaagatccg gactgcacga tattcatggg gctttcgggg gcgatgatcc
    12061 cggcaggtat gcagaacgtg ctcgtcgaac tcgtcaggca ccgctacgtc gatgttctcg
    12121 tctccacggg tgcaaacata ttccacgaca cctgcgagca cctcggcgtc cggcactaca
    12181 tcgggcacca ccacgccgac gacgcggcgc tctttgagga ggggatcgac cggatatacg
    12241 acgtctttgc gtatgaggaa gagttccgga gcgtcgatgc cgagatcgcc cggttcgccg
    12301 acgagatagc gcccttccgc ggctcgtcga gagagttcat ccggcgcctc gggggctggc
    12361 tttgcacacg gagaccggaa ggccgatcgc tcatcgccac ctgtgcggag tacgacgtcc
    12421 cgatcttcat ccccgccctc ggcgactcgt cgatcgggat cggcctcgtt atggcccgcc
    12481 ggcgcggtgt cgacgtcgac gtcgaccagc tcgccgacac cgacgagatc acccggatgg
    12541 tcgaggagtc gcagaagacc ggcgtcatat acgtcggcgg cggcgtcccg aagaacttca
    12601 tccagcagac ccaggtcatc gcctcgatcc acgagcagca cctcggcggg catgcctacg
    12661 ccgtccagta caccaccgac gccccccact ggggcgggct ttccgggtgc acgtttgaag
    12721 aagcgatcag ctggggcaaa gaggctcccg agtcccccag ggtccagtgc ttctgcgacg
    12781 caacgatcgc gctccctctc gtcgcgtcgg cactcatcgg gagcggggtc gagcgggcac
    12841 gccggtctcc ccgaagagag gcgtgatcgg gcgggccgcc ggagccggga gggagtacgg
    12901 cccggcaccc gggcgccggg accgtcgtgc cgcctggaat cccggatcac cacaattatt
    12961 attaattcat ctcgccaaaa ctagtaagca caagtcatag aatgctgtgt ctggagcagc
    13021 acccgcgtgc tgcgagtgtc gagagatgcg aagttctatg ttgaggtagc caagcctggt
    13081 atggcgcagg tttgctaa
//
LOCUS       DAOSEE010000054        13070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1851_length_13070_cov_229.192470, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000054 DAOSEE010000000
VERSION     DAOSEE010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 13070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13070
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1851_length_13070_cov_229.192470"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="PLI76_05630"
     CDS             complement(<1..578)
                     /locus_tag="PLI76_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylthioadenine synthetase"
                     /protein_id="HOI61546.1"
                     /translation="MCSKEIGRAGAEYSPAERESIQGREAAERESFTIAIDDLRERKV
                     YIESYGCTYNHADARRLAAILEGLGCTLTGPDEADAVIVNTCTVIEATERKMLRRLAL
                     FAGSDLYVTGCMPLVQMEAIRSVCTPHVILPAEIQERAGSVGSRGTGAIGVVQVAGGC
                     VGRCSYCITRLARGRLVSAPSEDILDAVRHLVA"
     gene            613..1371
                     /gene="nadX"
                     /locus_tag="PLI76_05635"
     CDS             613..1371
                     /gene="nadX"
                     /locus_tag="PLI76_05635"
                     /EC_number="1.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HOI61547.1"
                     /translation="MIKIGLLGCGNVGRIIATRAESIQVVAVFDIIPGRAEELAALCR
                     ARPYTDFDAFMREDFSIVVEAASVDAVKLYGEAVLRSGRDIAVLSGGALADEEFRERL
                     VETARAAGRKIRIPSGAIIGLDNLKIGQVSPPRRLLLRTTKPPASLGLSVAAPTEVFK
                     GLAHDCIKQYPKNINVAVALGLAAGRDADVELRVDPAAERNIHEIFAEGDFGEIYIQV
                     RNVPSPDNPATSYMAALSILTLLKNLDNPLVVGT"
     gene            1374..2267
                     /gene="nadA"
                     /locus_tag="PLI76_05640"
     CDS             1374..2267
                     /gene="nadA"
                     /locus_tag="PLI76_05640"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOI61548.1"
                     /translation="MEAKIRALKAEKNAVVMAHNYQPLDIQALADVVGDSLELAVKAK
                     ETGADLIVVCGVRFMAETAKILNPDRKVVIPVEDAGCPLADFLTPDLIREAKERYPDA
                     AVVVYVNSTAESKALADITCTSANAVRVVMSLPNETILFGPDANLAAYVQRELPKKTI
                     VPVPPGGHCYVHTGFTLADVEAARRKGGTVVCHPECLPEVQEQADVIASTGGMVRGAA
                     GAGEPWSVFTERDMVSRLRVLYPGRVFYEKPEAICVDMKKISPADLQRALECEEHEII
                     LPPGVMDRARRALERMIAVGA"
     gene            2274..3128
                     /gene="nadC"
                     /locus_tag="PLI76_05645"
     CDS             2274..3128
                     /gene="nadC"
                     /locus_tag="PLI76_05645"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOI61549.1"
                     /translation="MIPIEDLLRFIREDAPWGDVTSETVVPDVTCRAIVRAKSQGVVA
                     GLSEARRLFEHFGVTVRERSADGRAVAPGTVLLDLDGPARAILLVERTALNIIGRMSG
                     IATRTREAVEAVRAVSPDVRVAATRKTAPGLRVLDKKAVILGGGDPHRYTLSDMVLIK
                     DNHLALTPLAEAIRRAKKESLYRVVEVEVETVEDAVTAAGAGADIVLLDNMTPDTVRE
                     AVRALAGRGLRDRLTLEVSGGVGEDTLVGYAATGVDIISMGALTHTVRNFDVSLDILK
                     GMGIVRIP"
     gene            complement(3132..3557)
                     /locus_tag="PLI76_05650"
     CDS             complement(3132..3557)
                     /locus_tag="PLI76_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61550.1"
                     /translation="MKSPTGTSEGTTFPPDLERLGIAPGTRIDIRDLDAMGKRHNFHV
                     YLYFEEDLAKDSTLQEDLQEYCDIPDLERPFIRLDAFLRFATESDPLFVRRLDELPLV
                     VEIVAYGEIGIREGKTTPYVKGVMPFLDELAMEELPDAS"
     gene            3649..4203
                     /locus_tag="PLI76_05655"
     CDS             3649..4203
                     /locus_tag="PLI76_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOI61551.1"
                     /translation="MRWPMAGKGASISGGEAADHGNEERLHRHLAMLESGDLNQRWRA
                     ADALGEMGDRRAVQPLIRALSDEFVDVRWKAARALGIIDEREPVLPLIRSLEDESSWV
                     RESAAWALGTIGDPRAVEPLIGVLADTKPRVRREAARALGRIGIPRAREPLVRLLGDP
                     DRGVRLAARAALDDIGTEREIPSV"
     gene            4378..4812
                     /locus_tag="PLI76_05660"
     CDS             4378..4812
                     /locus_tag="PLI76_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOI61552.1"
                     /translation="MDLFMKCAIEEAEMGLREGGIPIGSVLVRGGRIIGRGHNRRVQE
                     NDPVIHAEIDCLRNAGRIGTYADCTLYSTLMPCYLCAGAVVQFGIRKVVAGESANFAG
                     ARELLESHGVEVIDLDLDVCKTMMHAFIEAYPDLWYEDIGEL"
     gene            4827..5633
                     /gene="budA"
                     /locus_tag="PLI76_05665"
     CDS             4827..5633
                     /gene="budA"
                     /locus_tag="PLI76_05665"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HOI61553.1"
                     /translation="MADTFLRYTAVAVAMFVVGASVALLAAPHPESSADRETVFLVST
                     IDALLQGAYDGSVSFDELAAHGDTGIGCGDRLDGELIGLDGEWYLIRTDGRAYPVAGS
                     ATTPFAAATFFDPDVAVAIDEPVNLTALLGRLETMFPSENLFYAVRLDGTFPRVVTRS
                     VPAQEKPYPRLADVVANQTVFELENVTGTAVGFWTPALAKGINVPGYHLHFITSDRTA
                     GGHLLDMTVAEGSVQLDTTANFTMVLPSRGDFLKVDLSGDLSGDLERVEK"
     gene            complement(5746..6195)
                     /locus_tag="PLI76_05670"
     CDS             complement(5746..6195)
                     /locus_tag="PLI76_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOI61554.1"
                     /translation="MKILVLIDGSKWSQKAALHAIEVAKQKNGEVVLFSVLDIKEAKA
                     MAFNFCTQSGICDRLQDYEGRIWADMRKSTEGDIAGILSRSREENIRCSSKIVEGRVR
                     EEVVNEANSGGYGLVVMGAFGKSGKARIGALIEDIGGVIRPPLLIVR"
     gene            complement(6201..7523)
                     /locus_tag="PLI76_05675"
     CDS             complement(6201..7523)
                     /locus_tag="PLI76_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HOI61555.1"
                     /translation="MDVLALIAVAVFLFTYALIIDERIHRAVAAMFGAAIVVFLGVIP
                     WEGLLEHVDFGTIFLLLGMMIIVNTARGSGLFEYIAIRTAKLANGSPIRILILFALVT
                     AVVSAFLDNVTTVLLLTPMLLYVARVMKLNPVPFLVTEIFASNIGGAATLIGDPPNIM
                     IASSAGLTFNEFLIHLGPIMAVDMVILLLMMYVIYGRSMKVSPGEREEMVRTLDSLDE
                     RAAITDRVLFRQSVIVIAFVVLLFFIHDRIGEILHVVLPFVDPAMGLEPAEVALIGAA
                     VLLFWSRQSPEEIFEKIEWPALFFFGGLFVIVGALVETGIIASIAAVMIENVGSVGEA
                     MFIVTWFAAIASAIVDNIPLTAAMIPLIHDLGTTMDVYPLWWSLALGACLGGNGTAIG
                     ASANVVVIGIAEREGIGITFIDFLKVGMLVLFVTVGVGFGMLWLTFGM"
     gene            complement(7535..8059)
                     /locus_tag="PLI76_05680"
     CDS             complement(7535..8059)
                     /locus_tag="PLI76_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOI61556.1"
                     /translation="MIRRKFKDIAGRRYEAVLKDYSEFLLTDKEMRVPEVRSILIPLD
                     LFVHDITDEAIDVLSAYNATITLAYITDAGVIELLEQALNHASTEEFRAKKEEYGREL
                     LDRTMAKLGDHGFMVQTRMFVGHKGDDVVRMAKNHDMVALCRRYADGESTETSVSPIV
                     LRICQRVETPALIY"
     gene            complement(8184..8714)
                     /locus_tag="PLI76_05685"
     CDS             complement(8184..8714)
                     /locus_tag="PLI76_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaemetzincin family Zn-dependent
                     metalloprotease"
                     /protein_id="HOI61557.1"
                     /translation="MGINILWDRQAPEGFELPVARMMTMILGREIGFVEYPFLIDGYD
                     RNRNQHDAQKILDRLQDTLTRRYEVAGPLLLVTSRDLYINGCDFVFGLARPASGVAVV
                     STARLGNGYYGRTPDDADLIDRTAKEGAHEIGHLLGLGHCADPECVMFRPQTLDELDR
                     KRKMLCPACREAAGSR"
     gene            8732..9139
                     /locus_tag="PLI76_05690"
     CDS             8732..9139
                     /locus_tag="PLI76_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0146 family protein"
                     /protein_id="HOI61558.1"
                     /translation="MRHYKRIERGIGGYIAGRYRCAVEVGIGNNPDAARVIAAAGGLL
                     LCTDIRPGIRHDGLTVVTDDIFEPDARLYAGADLLYAVRPGVEMVPPLIALAARINCD
                     LLVYHLGCEIYGDGGDVVDCGPALLHRYHRRRS"
     gene            complement(9136..10503)
                     /locus_tag="PLI76_05695"
     CDS             complement(9136..10503)
                     /locus_tag="PLI76_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication factor C large subunit"
                     /protein_id="HOI61559.1"
                     /translation="MDWAEKYRPQRLRDVVGNAGAVRQMYEWARDWSRQKKPLILYGK
                     PGIGKTSSAYALANDMNWEVVELNASDQRTKSALERVAGSGSTTASLLGASRKLILLD
                     EADNLHGQADRGGAKAIVEIIAASQQPIILIANDYYSLSRDLKAATEPVQFRALQARS
                     IVPRLRHICTAEGVTCDPAALDGIAERAGGDMRAAVNMLYAAAIGKESLAAGDVHTSA
                     KDERSTIFELVGAVFKGQDDADLLRTAMEVEDTPDTIVQWLEGNIDYIPDLASREKGY
                     ACLARADGYIGRTFRRQYYTLWRYASAVMLLGVADAAGGRGVRGRIMPPSRWQKMGAS
                     KKQKAVRAGLTRKLSNMAHIPEAALREDLFTAIGILVEQDPKSYVREFELDADELNLF
                     IHDKARAAKIVKELAKEEKAKEKKAGGKEKAAGKEKAPARKTRGDDPPGEARPDLPQG
                     QATLF"
     gene            10740..11750
                     /locus_tag="PLI76_05700"
     CDS             10740..11750
                     /locus_tag="PLI76_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 2 protein"
                     /protein_id="HOI61560.1"
                     /translation="MVKDCSTDTVAQAVREYEGVTRKRAIGDMVRSLRIENPDIVASF
                     GEDAAVIRHNGADALLLAADGIWSKLMEADPFWAGYCAVLVNVHDIAAMGGRPVAMVD
                     ILSVTNDAIRDNVTRGMVAASAQFGVPIVGGHLHPETPYSVVDVAILGTARMDHLIFS
                     DTAEVGDRVVAAIDLDGRVHPSCCFNWDSVTMKSAEEVRAQIRVMEDLGRDRLVTAGK
                     DISNPGVIGTLGMLLEMSGKGAVVDLEAIPRPDLSALDLSFEQWVRMYPGMGFILTVK
                     EENAEEVCRRFTDVGITASLIGEVNGSRKLTIRYLGRETQVFDLDRDGIMQIFSEGDV
                     CR"
     gene            11741..12502
                     /gene="mtxX"
                     /locus_tag="PLI76_05705"
     CDS             11741..12502
                     /gene="mtxX"
                     /locus_tag="PLI76_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker protein Mmp4/MtxX"
                     /protein_id="HOI61561.1"
                     /translation="MPVTVGLGAASPDEKIRATVRAVGREVDLTLFSCPGTADSFGDA
                     VGVVEAVHPEAALVEALAAGDIDAAVRGTLPASSTLAALKAAAGVDHLERIALLETAD
                     GHLFLLAPVGVDEGWTVADKVRFVEHGRHIARSFGLAGGVAVLSGGRLGDIGRHPAVD
                     RTMADAELVARLTGAEHVEILIEEAARRYGVVIAPDGISGNLIFRTLAFLGAGAGHGA
                     PVVNIDKIFVDTSRASPDYTNAIMLAKSLAESRKP"
     gene            12690..12896
                     /locus_tag="PLI76_05710"
     CDS             12690..12896
                     /locus_tag="PLI76_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone family protein"
                     /protein_id="HOI61562.1"
                     /translation="MADLPIAAVVRIAKKNGAERVGSDAAAALVLKAEAYIAELTKEA
                     NRLAQHAGRKTIKAEDVELAVKSA"
BASE COUNT         2321 a         4081 c         4235 g         2433 t
ORIGIN      
        1 gccacgaggt gccggacggc atcgagtatg tcctccgaag gcgcacttac aagccgcccc
       61 cgtgcaagcc gggtgatgca gtaactgcac cggccgacgc acccgcctgc cacctgcacc
      121 acgccgatcg cgccggtgcc gcggctgccg acgctcccgg cacgctcctg gatctcggcc
      181 gggaggatga cgtgcggcgt gcagaccgac cgtatcgcct ccatctgcac gagcggcatg
      241 cacccggtca cgtagaggtc ggagccggcg aagagggcaa ggcgccggag catctttcgc
      301 tcggtcgcct cgatcaccgt gcaggtgttg acgatcaccg cgtccgcctc gtccggcccg
      361 gtcagcgtgc acccgagccc ctccagtatc gccgcaagcc tccgggcgtc ggcgtggttg
      421 taggtgcacc cgtaactctc gatgtagacc tttctttccc tcagatcgtc gatggcgatg
      481 gtaaacgact ccctctctgc ggcctctctg ccctgtatgc tctctcgttc ggccggagaa
      541 tactctgccc ccgcccggcc gatctctttt gagcacaata cttaaccgct actattacaa
      601 cttctttact caatgattaa gatagggttg ctgggatgcg gcaacgtcgg gcgtatcatc
      661 gccacgcgcg ccgagagcat ccaggtcgtc gcggtcttcg atatcatccc cgggcgggcg
      721 gaggagctcg cggcgctctg ccgggcccgg ccatacacgg actttgacgc cttcatgcgg
      781 gaagacttct cgatcgtcgt ggaagccgcc tcggtcgacg ccgtcaaact ctacggcgag
      841 gcggtcctcc ggtcgggacg ggacatcgcc gtcctctccg ggggagcgct cgcggacgaa
      901 gagttccgcg aacgcctcgt cgagaccgca cgggcggcgg gaaggaagat ccggataccg
      961 agcggcgcca tcatcgggct tgacaacctc aagatcggcc aggtctcgcc gccgagaaga
     1021 ctcctcctcc ggacgacaaa accccctgcg tcgcttggcc tctccgtcgc ggccccgaca
     1081 gaggtattta aggggctggc gcacgattgt ataaagcaat acccgaagaa catcaacgtc
     1141 gcggtggcgc tgggactcgc tgccggccgg gatgccgatg tggagctccg tgtcgacccc
     1201 gcagcggaga ggaacataca cgagatattc gccgagggcg acttcgggga gatctatatc
     1261 caggtcagga acgtcccgag ccccgataac cccgccacga gttatatggc ggccctctcc
     1321 atcctgacac tcttgaagaa cctcgataac cctctggtgg tgggcacata ggaatggaag
     1381 caaagattcg tgcgctcaag gccgagaaga acgcagtcgt catggcgcat aactaccagc
     1441 ccctggatat ccaggcgctc gccgacgtgg tgggcgacag cctcgagctt gcggtgaagg
     1501 caaaagagac cggggcggac ctgatcgtcg tctgcggcgt ccggttcatg gccgagacgg
     1561 caaagatctt aaaccccgac cggaaggtcg tcataccggt ggaggacgcg gggtgccccc
     1621 ttgccgactt cctgaccccc gacctcatcc gggaggcaaa agagcggtat cccgacgccg
     1681 ccgtggtggt ctacgtcaac agcacggcgg agagcaaggc gctcgccgat atcacctgca
     1741 catccgcaaa cgcggtcagg gtcgttatgt ctctcccgaa cgagaccatc ctcttcgggc
     1801 cggatgcaaa ccttgcggca tacgtccagc gcgagctccc aaaaaagacg atcgtaccgg
     1861 tgccgcccgg cggccactgc tacgtccaca ccgggtttac ccttgccgac gtcgaagcgg
     1921 cccggaggaa gggcggtacg gtcgtctgcc accccgagtg cctgccggag gtccaggagc
     1981 aggcggacgt gatcgcatcc acggggggca tggtccgggg tgcggcggga gccggcgagc
     2041 cctggtcggt cttcaccgag cgggatatgg tctcccgcct ccgggtgctc tacccgggaa
     2101 gggtcttcta cgagaaaccg gaggcgatct gcgtggatat gaagaagata tcccccgccg
     2161 atctgcaaag ggcgctcgag tgcgaggagc acgagatcat cctccctccc ggggtcatgg
     2221 accgggcgcg gcgggctctc gagcggatga ttgccgtcgg ggcgtgagtg cgtatgattc
     2281 ctatcgagga cctgctccgg ttcatccggg aggatgcgcc gtggggcgac gtcacgtcgg
     2341 agacggtcgt ccccgacgtc acctgccggg cgatcgtccg ggcgaagagc cagggggtcg
     2401 tcgcggggct atctgaggcg cgacggctct ttgagcactt cggcgtcacg gtccgcgagc
     2461 gctccgccga cggcagggca gtcgccccgg gaacggtcct cctcgacctc gacggccctg
     2521 cccgggcgat cctcctcgtg gagcggacgg cgctcaacat catcgggcgg atgagcggta
     2581 tcgcgacccg gacccgtgag gcggtcgagg ccgtccgggc ggtctcgccg gacgtccggg
     2641 tcgccgcgac cagaaagacg gctccgggcc tccgggtgct cgacaagaaa gcggtgatcc
     2701 tcggcggggg cgacccgcac cgctacacgc tctcggatat ggtcctgatc aaggataacc
     2761 acctcgcgct cacgcccctt gcggaggcga tacggagggc aaagaaggag agcctctacc
     2821 gggtggtcga ggtggaggtc gagacggtgg aggatgcggt cacggcagcc ggcgccgggg
     2881 ccgatatcgt cctgctcgac aacatgaccc ccgataccgt ccgggaggcc gtccgggcgc
     2941 ttgccggccg gggtctccgg gaccgcctga ctctcgaggt ctccgggggg gtcggggaag
     3001 acaccctcgt cgggtatgcc gcaaccggcg tagatatcat cagcatgggt gcgctcaccc
     3061 atacggtcag gaacttcgac gtgagcctgg atatcctgaa aggtatgggc atagtccgga
     3121 tcccgtgacc ttcaggatgc gtccgggagt tcctccatcg cgagttcgtc gaggaacggc
     3181 atgaccccct tgacgtaggg cgtggtcttc ccctcccgta tccctatctc cccataggcg
     3241 acgatctcca cgaccagcgg gagttcatcc aggcgccgga cgaagagagg gtccgactcg
     3301 gtggcgaacc gcaggaaggc gtccagcctg atgaacgggc gctccaggtc cgggatgtcg
     3361 cagtactcct gcaggtcttc ctggagcgtg gaatcctttg ccaggtcttc ctcgaagtag
     3421 aggtagacgt ggaagttgtg gcgttttccc atcgcatcga gatccctgat gtcgatcctc
     3481 gtgccggggg cgataccgag gcgttccagg tccggcggga acgtggtgcc ctccgatgtg
     3541 ccggtcggac tcttcatact atggtgaagg ggatgatagt agaaaaaagc acctcccggg
     3601 gcaccctccg ggaggtaaaa ccttatactc cggcgctcca ggggggacgt gagatggcct
     3661 atggcgggga agggtgcatc gatatcgggg ggagaggctg cggaccatgg caatgaggag
     3721 cggctccacc ggcacctggc gatgctcgag tccggagatc tcaaccagcg ctggcgggct
     3781 gcggatgcgc tcggggagat gggggatcgg cgtgcggtgc aaccgctgat ccgggcgctc
     3841 tcagacgagt tcgtcgatgt ccggtggaag gcggcaaggg cgctcggtat catcgatgaa
     3901 cgcgaaccgg tgctcccgtt gatacggagc ctggaggacg agagctcctg ggtccgggag
     3961 agtgcggcct gggcgcttgg aacgataggc gacccccggg ccgtcgagcc gctgataggg
     4021 gtgcttgccg atacaaaacc ccgggtccgc cgggaggcgg cgcgggcgct cggccggata
     4081 ggaatcccgc gggccaggga accccttgtg cgcctcctcg gcgatcccga ccgcggcgtc
     4141 aggcttgccg cccgggcggc gctcgacgat atcgggaccg agcgggagat cccgagcgtc
     4201 tgacgggctg tttcctgctg gtcgccagac tcgcgtggtc gaccgcaccg cctctctctc
     4261 cgggaggccg gtaacccgtt tctctcccgg atataccctc gatccagaaa cgacttccgc
     4321 tattcttccc ggacattgcg attgatttat atatccggcc ctccggaaat ctattgaatg
     4381 gacttattca tgaagtgcgc catcgaggag gcggagatgg gcctgcggga ggggggaata
     4441 cccatcggat cggtgctggt ccggggcggc cgcatcatcg gccgggggca caaccgccgt
     4501 gtccaggaga acgaccccgt gatccatgcc gagatcgact gcctgaggaa cgcgggccgg
     4561 atcggcacct acgcggattg cacgctctac tcgacgctga tgccctgcta cctctgtgcg
     4621 ggagcggtcg tccagttcgg tatccggaag gtcgtggccg gggagtcggc caactttgcg
     4681 ggagcccggg agctccttga gtctcacggg gtcgaggtga tcgacctgga tctcgacgtc
     4741 tgtaagacga tgatgcatgc gttcatcgag gcgtaccccg atctctggta cgaggacatc
     4801 ggggagctgt gagcggtcgg ggctccgtgg cagatacgtt cctccgttac accgccgtag
     4861 ccgttgccat gttcgtcgtc ggggcctccg tcgcccttct ggccgctccc cacccggaga
     4921 gcagcgccga ccgggagacg gtttttttgg tctcgacgat cgatgcgctc ctgcagggcg
     4981 cctacgacgg cagcgtctcg tttgacgaac ttgcggcgca cggcgatacc ggcatcgggt
     5041 gcggcgaccg tctcgacggg gaactgatcg gccttgacgg cgagtggtac ctgatccgga
     5101 ccgacgggcg ggcctacccg gtcgccggga gcgcgacgac gccgtttgcg gccgcgacgt
     5161 tctttgatcc ggatgtagcg gtcgcgatcg acgaaccggt gaacctgacc gctcttctcg
     5221 gccgcctgga gactatgttc ccctcagaga acctctttta cgccgtcagg ctggacggca
     5281 ccttcccgcg ggtggtgacc cggagcgtgc ccgcccagga gaaaccctac ccgcggcttg
     5341 ccgacgtggt ggcaaaccag acggtcttcg agcttgaaaa cgtcaccggg accgcggtgg
     5401 ggttctggac gcccgcgctg gcgaaaggga tcaacgtccc cgggtaccac ctccacttca
     5461 tcacgagcga ccgcaccgcc ggcgggcacc tgctcgatat gaccgtggca gagggcagcg
     5521 tgcagctcga taccacggca aacttcacga tggttcttcc ttcccgcggc gatttcctga
     5581 aggtcgacct ctcaggcgac ctctcgggcg accttgagcg ggtggagaag tagcgcgccc
     5641 gagggcaggg cgctgccctc cgcccccccg gggggccggc gggaagccgt gcgcctatcc
     5701 ggagcgcgga cgcaaccctg ccacgggtgg gccgaaaaga gatgtttacc ggacgatgag
     5761 cagcggcggc ctgatcacgc caccgatatc ctcgatcagg gcgcctatcc gggccttccc
     5821 gctcttgccg aaggccccca tgacgaccag cccgtatccg ccggagttcg cctcgttgac
     5881 gacctcctcc ctgacgcggc cctcgacgat ctttgacgaa caccggatat tctcctcccg
     5941 ggagcgcgag aggatgccgg cgatgtcacc ctcggtgctc ttgcgcatat ccgcccagat
     6001 ccgaccctca taatcctgca gccggtcgca tatgccgctc tgagtacaga agttgaacgc
     6061 catcgccttg gcctccttga tatccagcac cgagaagagg accacttcgc cattcttctg
     6121 ctttgccacc tcaattgcgt gcagagccgc tttctggctc cacttcgaac catcgatcag
     6181 cacgagaatc ttcatagtgt tcacatccca aatgtcagcc agagcatccc gaacccgact
     6241 cccaccgtca cgaagagcac cagcatcccc accttcagga aatcgatgaa ggtgatgccg
     6301 atcccttcgc gctcggcgat accgatgacg acgacgtttg ccgacgcccc gatggcggta
     6361 ccgttcccgc cgaggcaggc gccgagggcg agcgaccacc agagcgggta gacatccatc
     6421 gtcgtcccga ggtcgtgtat gagcggtatc atcgccgcgg tcaggggaat gttgtccacg
     6481 atcgccgagg cgatcgcggc aaaccaggtc acgatgaaca tcgcctcgcc aaccgatccg
     6541 acgttctcga tcatgaccgc ggcgatgctt gcgatgatac ccgtctcgac gagggcgccg
     6601 acgatgacga agagcccgcc gaagaagaag agggccggcc actcgatctt ctcaaatatc
     6661 tcctccggcg actgccggct ccagaagagg aggaccgccg ccccgatgag ggccacctcc
     6721 gcaggctcaa gccccatcgc cgggtcgaca aacggcagga cgacgtgcag gatctccccg
     6781 atacggtcgt gaataaagaa gaggaggacc acaaacgcga tgacgatcac cgactgccgg
     6841 aagagcaccc ggtcggtgat cgccgcccgt tcatcgaggc tatcgagcgt ccggaccatc
     6901 tcctcccgct cgccgggact caccttcatg gatcggccgt atatgacgta catcatcagg
     6961 aggaggatca ccatatcgac ggccatgatg gggccaagat ggatcaggaa ctcgttgaac
     7021 gtcagtcccg cagacgacgc gatcatgatg ttcggggggt cgccgatgag cgtcgccgca
     7081 ccgccgatgt tcgaagcaaa tatctccgtg accaggaacg ggacagggtt gagtttcatc
     7141 acccttgcga cgtagaggag catcggtgtg aggaggagga ccgtcgtcac gttgtcgagg
     7201 aacgcgctca ccaccgcggt cacgagcgca aagagaatga ggatccggat cgggctcccg
     7261 ttcgcgagtt tcgccgttcg tatggctatg tactcaaaga gaccgctccc ccgggcggtg
     7321 ttgacgatga tcatcatgcc aaggagcagg aagatcgtcc cgaagtcgac atgctcgagg
     7381 agcccctccc aggggatgac cccgaggaag acgacgatcg ccgccccgaa catggcggcg
     7441 accgcccggt ggatccgctc atcgatgatg agcgcgtagg tgaagaggaa gaccgctacg
     7501 gcgatcagcg cgagaacatc catcgatgca cccgtcagta gatgagtgcc ggggtctcca
     7561 cccgctggca gatgcgcagg acgatcgggc tgaccgacgt ctcggtcgac tcgccgtcgg
     7621 catagcgccg gcagagcgcg accatgtcgt gattcttcgc catccggacg acgtcgtcgc
     7681 ctttatgtcc gacaaacatc cgtgtctgga ccataaatcc atggtcaccg agttttgcca
     7741 tggtccggtc gaggagttcc cggccgtact cttccttctt tgcccggaac tcttccgtcg
     7801 atgcatggtt gagcgcctgt tcgaggagtt cgatcacccc ggcgtcggtg atgtaggcga
     7861 gcgttatggt ggcgttataa gcagagagga catcgatcgc ttcgtcggtg atatcgtgga
     7921 cgaagaggtc gagcgggatc aggatcgatc ggacttccgg gacgcgcatc tctttatcgg
     7981 tcagcagaaa ttcgctgtaa tccttgagga ccgcttcgta gcgccttccg gcgatatctt
     8041 tgaactttct tcggatcaag gaagatgagt atcccatggt gattcacttc tacgttttcc
     8101 agagcttccg tttaatactt tgcccgtccg accctgccgg acacccctgg agaacaccgc
     8161 gatgcaaccc ggagcggttt ccctcaccgg gagcctgccg cctccctgca ggccggacag
     8221 agcatcttcc tcttccggtc cagttcatcc agcgtctgcg gacgaaacat gacgcactcg
     8281 gggtcggcgc agtgcccgag gccgaggaga tgcccgatct cgtgcgctcc ctcttttgcc
     8341 gtccggtcga tcaggtcggc gtcgtcgggc gtcctgccgt agtagccgtt cccgaggcgg
     8401 gcggtcgaga cgacggcaac gccgcttgcc ggccgtgcga ggccgaagac gaagtcgcac
     8461 ccgttaatat agaggtcgcg ggaggtaacg aggagcaggg ggccggcaac ctcgtacctg
     8521 cgggtgaggg tatcctgcag gcggtcaagg atcttttgag catcgtgctg gttgcggttc
     8581 ctgtcgtagc cgtcgatgag gaacggatac tcgacgaatc cgatctctct tccaaggatc
     8641 atcgtcatca tccgggcgac cgggagctcg aagccttccg gggcctgccg gtcccaaaga
     8701 atattgatgc ccattgccta atctctgggt aatgcgtcac tataaacgta ttgaacgggg
     8761 tatcgggggg tatatcgccg gccgctaccg gtgcgccgtg gaggtgggca tcggcaacaa
     8821 ccccgatgcc gcccgcgtga tcgcggccgc cggcggcctt ctgctctgca ccgacatccg
     8881 gcccggcatc cgccacgacg ggctcaccgt ggtgaccgac gatatattcg agcctgacgc
     8941 gaggttatac gcgggcgcgg acctgctata cgccgtgcgc cccggcgtgg agatggtccc
     9001 gccgctgatc gcgcttgccg cccggatcaa ttgcgacctc ctggtctacc acctggggtg
     9061 cgagatatac ggggacggcg gcgatgtcgt ggactgcggc ccggccctcc tccaccgcta
     9121 ccaccgccgc cgctcttaaa agagggttgc ctggccctgc gggaggtccg ggcgcgcctc
     9181 tccgggcggg tcgtcgcccc gggtctttct ggcaggcgct ttctccttgc cggccgcttt
     9241 ctccttaccg cccgcctttt tctcttttgc cttctcctct tttgccagct ctttgacgat
     9301 ctttgcggcg cgggctttgt cgtggatgaa gaggttcagc tcgtcggcgt cgagctcgaa
     9361 ctcgcggacg taactttttg gatcctgctc gacgaggatg ccgatcgcgg tgaagaggtc
     9421 ctcgcggagg gcggcctccg gtatatgcgc catattgctg agttttcgcg tcagacccgc
     9481 ccgcaccgcc ttctgtttct ttgatgcccc catcttctgc cagcgcgacg ggggcatgat
     9541 gcggccgcgg acgccgcgcc cgccggcggc gtcggcaacg ccaaggagca tgaccgcgct
     9601 tgcgtaccgc cagagggtgt agtactgccg ccggaacgtc cggccgatat atccgtccgc
     9661 ccgcgcgagg caggcgtagc ccttctcccg ggaggcgaga tcggggatgt aatcgatgtt
     9721 gccctcgagc cactgcacga tggtgtcggg cgtatcttcg acttccatcg ccgtacggag
     9781 cagatcggca tcgtcctggc ccttaaagac cgcgccgacg agctcgaata tggtggagcg
     9841 ttcgtccttt gcggatgtgt ggacgtcgcc cgccgcgagg ctttccttcc cgatggccgc
     9901 ggcgtagagc atgttcaccg ctgcccgcat atcgccgccg gcgcgctcag cgataccgtc
     9961 gagtgcggcg gggtcgcacg ttaccccctc tgcggtgcat atgtggcgga ggcgggggac
    10021 gatcgaacgg gcctggagcg cccggaactg gaccggttcc gtggccgctt tgaggtcgcg
    10081 cgagagggag tagtagtcgt ttgctatcag gatgatcggt tgctgtgacg ccgcgatgat
    10141 ctccacgatc gccttcgccc cgccccggtc ggcctgtccg tgcaggttgt cggcctcgtc
    10201 gagcaggatg agctttcggc tcgcgccgag gaggctggcc gtggtcgatc ccgaacctgc
    10261 cacccgctcg agggccgact tcgtccgctg gtcggaggcg ttcagttcca ccacctccca
    10321 gttcatgtcg tttgcaaggg cgtacgcgct tgaggtcttg ccgataccgg gtttcccgta
    10381 caggatgagt ggtttcttct gccgcgacca gtcccgcgcc cattcgtaca tctgcctgac
    10441 ggcgccggcg ttccccacca catcccggag gcgctgcggc ctgtacttct ccgcccagtc
    10501 catggggatc tatacgacgc ggtgcatcaa aaataccacc ccggagggca gagcgactct
    10561 ggagcaacgt ggcgatggag cggcagggca accgcagggg gccttctccc gatctaaacc
    10621 gggcgggccg tgctgccggt tatcggcttc cggagcagag ttcataatac aggtgaatca
    10681 aatacattat ctctgatgag acagaatacc atacagaaag gtggagtgtt ggtgttctcg
    10741 tggtgaaaga ttgctccaca gatacggtgg cacaggccgt cagggaatat gaaggcgtta
    10801 cccggaagag agcgatcggg gatatggtcc ggtccctccg gatcgagaac cccgatatcg
    10861 tcgcctcgtt cggcgaggat gccgcggtca tccggcataa cggagcggat gcgttgctgc
    10921 ttgcggcaga cggcatctgg agcaagctca tggaggcgga cccgttctgg gcaggatatt
    10981 gtgccgtgct cgtgaacgtt cacgatatcg ccgctatggg cgggagaccg gttgccatgg
    11041 tcgatatcct ctctgttaca aacgacgcga tccgcgataa cgtgacccgg ggcatggtcg
    11101 ccgcatccgc ccagttcggc gtcccgatcg tgggcggaca cctgcacccc gagacgccgt
    11161 acagcgtcgt ggatgtggcg atcctcggga cggcccggat ggatcacctc atcttctcgg
    11221 atacggcaga ggtgggcgac cgtgtggtcg cggcgatcga cctcgacggc cgggttcacc
    11281 cgtcgtgctg cttcaactgg gactccgtca cgatgaagtc ggcggaagag gtgcgggccc
    11341 agatccgggt gatggaggac ctcgggagag atcgcctggt gacggccggc aaggatatca
    11401 gcaacccggg tgtcatcgga acgctcggga tgctccttga gatgagcggg aagggtgcgg
    11461 tggtcgacct tgaggcgata ccccggccgg acctctccgc gctcgatctc tccttcgagc
    11521 agtgggtgcg catgtacccc ggcatggggt tcatactgac cgtgaaggag gagaacgcgg
    11581 aggaggtctg ccgccggttt accgatgtcg gcatcaccgc ttctctcatc ggagaggtga
    11641 acgggagccg gaagctgacc atcaggtacc tgggccggga gacgcaggtc tttgacctgg
    11701 accgggacgg catcatgcag atcttctctg aaggggatgt atgccggtaa cggtcggact
    11761 tggtgcggca tcccccgatg agaagatccg tgcgaccgtc cgggcggtcg gccgggaggt
    11821 cgatctcact cttttttcct gtcccggaac cgccgattcg ttcggagacg ccgtcggggt
    11881 cgtcgaggcg gtacatccgg aggctgccct tgtcgaggct cttgccgccg gcgatatcga
    11941 tgccgccgtc aggggcactc tgccggcgtc ttccacgctt gcggccctca aggcggccgc
    12001 cggcgtcgac cacctggagc ggatagcgct cctcgagacc gccgacgggc acctcttcct
    12061 ccttgccccg gtcggtgtcg acgagggctg gacggtcgcg gacaaggtcc ggttcgtcga
    12121 gcacggccgc catatcgccc ggagtttcgg gcttgccggg ggggtcgcgg tcctctcggg
    12181 cgggaggctc ggggatatcg ggcggcaccc ggccgtcgac cggacgatgg cggacgccga
    12241 gctcgtcgcc cgcctgaccg gggcggagca tgtcgagatc ctgatcgagg aggcggcgag
    12301 gcgttacggg gtggtcatcg ctccggacgg gatatcgggg aacctgatct ttcgtacgct
    12361 tgcgttcctt ggagccgggg ctggacacgg tgccccggtg gtaaatatcg ataaaatttt
    12421 cgtggatacg tcgcgcgcct caccagatta tacgaatgct ataatgctcg cgaaatcgtt
    12481 agcggaatcc agaaaaccct gaataatact cccttttctg gaaatatccg taatccgggg
    12541 ttatttgaat cgtcacaatt ttacgccggg tatcaggatg ctctgcccat aagcgacgcc
    12601 gataagaaag agaaacgcaa attttcgaaa taatcccgaa atgtttaaat attaactcgt
    12661 acacctcttt ttagaggtga aatttgatta tggcagattt acctattgct gcggtcgttc
    12721 ggatcgcaaa gaaaaatggt gctgagaggg tcggcagcga tgccgctgct gcactcgtcc
    12781 tgaaggctga ggcgtatatc gccgagctga ccaaggaagc caacaggctt gcccagcacg
    12841 cggggcgcaa gacgatcaag gctgaggatg tcgagctcgc ggtcaagtcc gcgtaacccg
    12901 gctgctcact ctctctttct ctcttctcct taccgggggt ccgagagacc cccttctcaa
    12961 ggcccgtccc ggcggtgatg gtctctcccc gggggggccg gcgttttgtg gatatctcgt
    13021 cgtgacgcat accgggccat gcccatgctg gcaatgggcg tcagacgctt
//
LOCUS       DAOSEE010000055        13070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1852_length_13070_cov_217.407069, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000055 DAOSEE010000000
VERSION     DAOSEE010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 13070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13070
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1852_length_13070_cov_217.407069"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..617
                     /locus_tag="PLI76_05715"
     CDS             171..617
                     /locus_tag="PLI76_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOI61563.1"
                     /translation="MKDEDPAVKTGVPYWHVWTDEDGVSRQARRRLTDFAFASISEGA
                     APSWIKRLSTPAARVVVLVLPAGWVGEWHENPEPQWIVPLSGRWFVETMDGARVEMGP
                     GDLSFGGDQNTRPDAHGRRGHRSGTVGDEPAVLMLVQMKDAPGRPG"
     gene            complement(625..1806)
                     /locus_tag="PLI76_05720"
     CDS             complement(625..1806)
                     /locus_tag="PLI76_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOI61564.1"
                     /translation="MVYRFAGRMGRVPDSFLDELFRVSVVPGVISFAGGLPGSAYIDV
                     EGIRDAARDVFTDEGRTALQYTTTDGYLPLREFIADRYRTRLGLPATPEEIQIVNGSQ
                     QCLDLVAKIFLDPGDAVGMERPGYLGAIEAFSLYEPEFCSVPLTDDGPDLDRFASLVR
                     EHAPKFFYGIPNSQNPSGMTYSDDTRRGVADILEGTDTVFYEDDAFGELFFDGRPRPP
                     VKRYLPDRTVMSGSFSKIVAPGMRIGWIYAPAPILREFNVAKQAADLHSNFLCQAILH
                     RYLATHDLDAHVARVSAVYGRHCRLICDLLDDLMPPGTTHTTPEGGMFMMVTLPEGVS
                     STEVFSEGVREGVAVLPGVPFYVGGGGEDTIRLNFSAAGEEDIGEGMRRLARVVRRLA
                     A"
     gene            complement(1844..3583)
                     /locus_tag="PLI76_05725"
     CDS             complement(1844..3583)
                     /locus_tag="PLI76_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2070 family protein"
                     /protein_id="HOI61565.1"
                     /translation="MESSPDVRFERLTRYLFSAPSWPRSLLLIILLGVVIDIAAYRVG
                     NGFFLVGTLGFALPALVAFLLTVPLVEVFGRQITYNRSGLLALACTVLMVILSLSPVL
                     VFGRELFSALYAGVLGLATGIRLLVLVAVADYRITRMAAPALLQGVTGIAVGAWIFTP
                     GFVPYAILLQVVFGLVFVLLIGLIERPLKRAFQVSGLNFLNAFIAHLTDGSKSMEAFF
                     RDIGEEVCVQQASLFFSRDSGEDVLFTVPNVHPGPMGDVGGGNLPRVLHDTFAAETLV
                     AHGCATHDFNLVSESEIETIARAIEASGEGISFADTASRPVRVASGSVSILCQRFGDA
                     LLMVSTRSPERTEDLDYSIGMVIMAEGRCFFSEVAFVDAHNCMTSVGSPVLPATRIAM
                     EYIDAAREGFSAARDLPAEPLAVGVSHVRVPFTREQGFGSLGVQALVTDAGGKRAAYV
                     LVDGNNMAPGVRERLLEVVLGYADEAEIMTTDTHTVNTISGKNPVGYVVPVDAIAPYI
                     EQAVREAVADIAPARVGAATASCEGVVVFGSQRVSQLASTVNAMLAFIGPISLIILLL
                     AFLLSVFAYLLLQ"
     gene            complement(3649..4539)
                     /locus_tag="PLI76_05730"
     CDS             complement(3649..4539)
                     /locus_tag="PLI76_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOI61566.1"
                     /translation="MIAVVGHTAIDHLFSVPGLPGRHNSTYIVDHQVYFGGGAANIAA
                     GIATLGERCRLVSPVGGDFPGSDYDRWMRDLGIVQDFTLVKEARTATAYVFTDEAGDQ
                     ETFFEWGASAAFSRAEAPALDFVHMATADPDFNVRVAEKSRFASFDPGQDLLRYTPDQ
                     LEIILAKIDILFSNNHEMDRMCDMLGLERPALIASVPIVVTTRGAEGSVLCMDGEEHH
                     VPAVRVNAVDPTGAGDGYRAGFLTAYRRGYAPIDCCRAGAVVSSFVVERPGTQTNLPD
                     WERMLVRYRKVFGEPGEISP"
     gene            complement(4536..4874)
                     /locus_tag="PLI76_05735"
     CDS             complement(4536..4874)
                     /locus_tag="PLI76_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF555 domain-containing protein"
                     /protein_id="HOI61567.1"
                     /translation="MPDYLVTLESAWIIKDVKSMDDAVSIAISEAGKRLNPSAKFVDI
                     EAGMIACPYCEGELNSALVVANTALVGLVLQMKVFRAESGEHASRIAKSVVGKALRDV
                     PLKVQEVQEA"
     gene            complement(4895..5422)
                     /locus_tag="PLI76_05740"
     CDS             complement(4895..5422)
                     /locus_tag="PLI76_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOI61568.1"
                     /translation="MTSSDLLRLLKARSSVRDYTGEPLDPEDIDYILTCASTAPSAGN
                     REAWDVVVVTDEDLRIGLAVAALEQMHIREAPAIFVVCANYVRSMSQYGERGILYALE
                     DATIACTYMMLAAHARGLHSCWTGAFDENDVRETLGLPEHIRPLALLAVGKGIPPLRP
                     VERMPVEEHVHRETW"
     gene            complement(5409..5765)
                     /locus_tag="PLI76_05745"
     CDS             complement(5409..5765)
                     /locus_tag="PLI76_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61569.1"
                     /translation="MDLKTAVRTYQFAERAKSELIVGSQLTTALIQFPLQEKPGGKRM
                     LLMVLQSIRSELEFAYGDTEQREFRKAIDHLNEGISLTESMELGAASERMARAVSAVT
                     TAAQTAWDTLKEHDLL"
     gene            complement(5822..6130)
                     /locus_tag="PLI76_05750"
     CDS             complement(5822..6130)
                     /locus_tag="PLI76_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5350 domain-containing protein"
                     /protein_id="HOI61570.1"
                     /translation="MGKTGSVKWAQVKGVKGQIRLVPASEGEVKKPGPNQRFKPLADI
                     RKRASREGQDQRRGGRGGRGGRGGRGARGGGTPTMDVRVRRSIRRAKVSALGTKQKSR
                     "
     gene            complement(6218..8077)
                     /gene="gatE"
                     /locus_tag="PLI76_05755"
     CDS             complement(6218..8077)
                     /gene="gatE"
                     /locus_tag="PLI76_05755"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HOI61571.1"
                     /translation="MDYKDLGLKAGIEIHQQLDTAEKLFCRCPTLLRDVADRTGEFYR
                     YLRATESELGEIDRAAEEEMKLVRKFCYYTYDTVCLVDHDEEPPTPMNPEALDICLTI
                     AKMLDMTPVEQIHTMRKLVVDGSNTSGFQRTALVALSGALPDGCRIETICLEEEAAQR
                     VEGETFSLDRLGIPLVEITTAPCMRTPEAVQEVAAYIGMVLRSTGRVKRGLGTIRQDI
                     NVSIAGGARVEIKGVQELDLIAEVVRREVDRQTNLLAIRDALRDRGARVDRTVVDVTA
                     LFAGTKSAVLKKAKSILAIRLCGFAGLVGREIQPGRRLGSEMSDYAKKCGVGGIFHTD
                     ELPAYGVTAEEVARLREFVGAGDDDCVAIVAASRERAGCAAEQVMIRADMALAGVPEE
                     TRKMLEEGSSAYMRPLPGAARMYPETDVFPIALSGALWEGIRVPELLTDRAERFVREF
                     GLDGALARQVAFSERLPLFERAVAAGVRPTLAARTLLATCRELARGGVAIDRVSEDDI
                     LALLSAVEAGRAAKEAIPDLLAELARTADESAGTPRERVDAAIAKMAPAVSQADVESI
                     VRRIVAEREAFAREKGMGALGPLMGVVMQELRGSVDGKVVSETLRRELKRLLS"
     gene            complement(8078..9310)
                     /gene="gatD"
                     /locus_tag="PLI76_05760"
     CDS             complement(8078..9310)
                     /gene="gatD"
                     /locus_tag="PLI76_05760"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HOI61572.1"
                     /translation="MTESLEAGDRVRYANGGTALTGIYIAERDGMAVIKLDSGYNIGA
                     SPEKLERVGRAAPQPPAGAGIVIQDPALPELSIISTGGTIASRIDYRTGAVTSQFSAN
                     DILRSIPELGDIARYRDRQVASILSENMRPAIWQALARAIYDEIRHGTSGVIVTHGTD
                     TMASSAAAVRFMLRTPVPVVFVGSQRSADRPSSDNAMNTLCSAAVAAGDLGEVAVVMH
                     ATTNDDRCAVHRATRVRKMHTSRRDAFQSMGMAPLGSVDYPSLAVTLSDEAVRRGAEE
                     PGLHDTLEERCALLQFYPGMPPGILDAFEGYAGLVIVGTGLGHVATDWIPKLRDRIEG
                     GTTVVMTSQCLHGRVCDRVYNTGRDLLRAGVIEGEDMLPEVALVKLMWVLGNEPDPER
                     AGVLMQTDLAGEIRRRSI"
     gene            complement(9307..10785)
                     /gene="argH"
                     /locus_tag="PLI76_05765"
     CDS             complement(9307..10785)
                     /gene="argH"
                     /locus_tag="PLI76_05765"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOI61573.1"
                     /translation="MRSDQIRQGRLSGERSADLEHFLASMAADRWIAEADLLVDMAHL
                     LGLSRQGIIDEAPARALMAALLDLHDHGLPEEAFDEHYEDIHAGKEAYLIGRAGEDFG
                     GRLHMGRSRNDEVATCIRIRLKQELIALVQSLAGLRRTLLDVAAAHTETVMPGFTHLQ
                     HAQPTTLAHHLLAYEQAFSRDTARLREAYARVDVSPLGSAAFASTGFPLDRDYTARLL
                     GFSRPAPNSMDAVAARDFAIEVLGDIAALMTTASRFCEELVIWSSAFVGFVHLDDAYC
                     SSSSIMPQKKNPDVAEIMRARAGSAAGSLIAAITIVKGLPMSYNRDLQELTPHLWRGV
                     EAARQSIPLLSGMIATAAFNTERMAAEAGRGFSTATELADVLVREYGLPFRTAHRIVG
                     RAVRHGSLDIATLEGAAREAAGLSVVDLGLTEERIALALDPRHAVAVRTIVGGPAPAA
                     VAVQLTEQEELLARDRAWAEETDTALSRAFEDLISESRRLIL"
     gene            11272..12258
                     /locus_tag="PLI76_05770"
     CDS             11272..12258
                     /locus_tag="PLI76_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOI61574.1"
                     /translation="MRSITATLLVVAMVAAFACIAGCTGTGADDMTHLRIGYQPSTHQ
                     IAHFTAMEKGWWQEDLAPLGITKVTDNQFGTGPPEMQAMLAGELDIAYVGAAPVVSAL
                     ASGLDAKIIAAVQTQGSDLMLRNDIAYESPQDLVGKEIATYHPGSIQDTLLRTWLEKN
                     GVDPAKVIIKPMDPGAATTAIAAGKVDGVFLPDPYPAIIIAEGTGRTIVQSGEMMKDH
                     ACCVLVATGSLIREHPDIVEQIVRTHIKATEYNFEHQDEAAELYSAKTGQNLDTVKAS
                     FKIWDGTWTADPRIIIPSTIEYTNLQYELGYIKKPLTQDDLFDLSFYEKARA"
     gene            12394..>13070
                     /locus_tag="PLI76_05775"
     CDS             12394..>13070
                     /locus_tag="PLI76_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI61575.1"
                     /translation="MNRQTQKRLLGITALVTVAVIWQIIAEYVVGRSFILPSVTDVIS
                     AFVDLIGSGTLLTDITVSLEHFGIGLLAAFFLGVPIGILMGWFRTADFLLDPIIEVFR
                     PIPPLAWIPFSIIWFGLTTQAAGFMIFVGAFFPILTATYTAFRSIPKVFVEAGKVLGC
                     NTSFELIRHVALPAAIPAIASGIRIAMGVGWMCLVGAEMFGVSNYGLGYQLWHNYYLH
                     QMPNVVV"
BASE COUNT         2301 a         4323 c         4289 g         2157 t
ORIGIN      
        1 atcttaagtc caccgccttt ggccgggctt ggctaccctc gctcaaaaga tccattacct
       61 attatctccg gcagatatta agcccagcgc ccggcaagag taaacccggg cagcccgacc
      121 ccggcggcac aacccgggcc gggccataca ccgcccggag aggcatcgtg atgaaagacg
      181 aggatcctgc cgtgaagacc ggcgtgccct actggcacgt ctggaccgac gaggacggcg
      241 tgagccgcca ggcccggcgc cgactgaccg atttcgcgtt tgcaagcata tcggagggcg
      301 ccgcgccgtc ctggataaag agactgagca ccccggccgc ccgcgtggtc gtcctggtcc
      361 tccccgcggg ctgggtcggc gagtggcacg agaaccccga gccgcagtgg atcgtgcccc
      421 tctcgggccg ctggttcgtc gagaccatgg acggcgcccg cgtggagatg gggccgggcg
      481 acctctcgtt cggaggcgac cagaacaccc ggcccgacgc ccatgggcgc cggggccacc
      541 ggtcggggac cgtcggcgat gagccggcgg tcctgatgct cgtccagatg aaagatgccc
      601 ccggcaggcc cgggtgaaaa tagttcaggc cgcaagcctg cgcaccaccc gggccagccg
      661 gcgcatcccc tccccgatat cctcctcgcc cgccgccgag aagttcaggc ggatcgtatc
      721 ctccccgccg ccgcccacgt agaacggaac cccggggagg accgcgacgc cctccctgac
      781 gccctcggaa aagacctccg tcgacgagac gccttccggc agcgtcacca tcatgaacat
      841 cccgccctcg ggggtggtgt gggtcgtccc cggcggcata aggtcgtcga ggaggtcgca
      901 gatcaggcgg cagtgccgcc cgtagaccgc agagacccgg gcaacgtggg cgtcgaggtc
      961 gtgcgtcgca aggtagcggt ggaggatcgc ctggcagagg aagttcgagt ggaggtcggc
     1021 ggcctgcttt gcgacgttga actcgcgaag tatcggagcc ggcgcgtata tccagcctat
     1081 ccgcatgccc ggggcgacaa tctttgagaa cgaccccgac atgaccgtcc ggtccgggag
     1141 gtagcgcttc accggcggcc gcggcctccc gtcaaagaag agctccccga aagcgtcgtc
     1201 ctcgtaaaag accgtatccg tcccctcgag gatatccgcg acgccccgcc gcgtatcgtc
     1261 cgaatacgtc atccccgaag ggttctgcga gttcggtatc ccgtagaaga acttcggggc
     1321 gtgctcccgg acgagcgacg caaaccggtc caggtccggc ccgtcatcgg tgagcggaac
     1381 cgaacaaaac tcgggctcgt agagcgagaa agcctcgatg gcaccgaggt agccgggccg
     1441 ctccatgccg acggcatccc ccgggtcaag aaagatcttg gcgaccaggt cgaggcactg
     1501 ctgcgaaccg ttcacgatct ggatctcttc aggcgtcgcc gggaggccga gccgcgtccg
     1561 gtaccggtcg gcgatgaact cccggagcgg caggtagccg tccgtcgtcg tgtactggag
     1621 cgccgtccgc ccttcatcgg taaagacatc gcgggccgca tcccggatcc cctcgacgtc
     1681 gatataggcc gaacccggca gcccccctgc aaacgatatc acgccgggaa ctaccgagac
     1741 ccggaagagc tcatcgagga acgaatccgg gacccgtccc atccggccgg caaaacggta
     1801 aaccatacga tcatattggg cgcgccggca aaaagatgcc ggcttactgc aacaggagat
     1861 acgcaaagac cgagagcagg aacgcgagca ggagtatgat gagactgatc gggccgatga
     1921 acgcgagcat ggcgttcacg gtgcttgcca gctgcgagac ccgctgcgac ccgaagacga
     1981 cgaccccctc gcacgaggcc gtcgccgccc ccacccgggc cggggcgata tcggcaaccg
     2041 cctccctgac cgcctgctcg atatacgggg cgatcgcatc gaccggcacc acgtagccga
     2101 ccgggttctt cccgctgatg gtgttcaccg tatgcgtatc ggtggtcatg atctcggcct
     2161 cgtccgcgta gccgagcacc acctcgagca ggcgctcccg gacgcccggg gccatgttgt
     2221 tcccatccac gaggacgtag gccgcccgct tccccccggc atcggtcacg agcgcctgca
     2281 ccccgagcga cccgaacccc tgttcgcggg tgaacgggac acggacgtgc gagacaccga
     2341 ccgcgagcgg ttctgcgggc aggtcacgtg cggcggagaa cccctcgcgg gcggcatcga
     2401 tgtactccat cgcaatccgc gtcgccggga ggacggggga gcccacgctg gtcatgcagt
     2461 tgtgggcatc cacgaacgcg acctccgaga agaagcaccg cccttcggcc atgatcacca
     2521 tgccgatcga gtagtcgagg tcctccgtcc tctccggcga ccgggtgctc accatgagga
     2581 gagcgtcccc gaaccgctgg cagaggatcg agaccgaccc cgacgcgacc cggacagggc
     2641 ggctcgccgt atcggcaaaa gagatcccct cgccggacgc ctcgatggcc cgggcgatcg
     2701 tctctatctc gctctccgag accaggttga agtcgtgggt ggcgcacccg tgggcgacga
     2761 gggtctccgc agcaaacgta tcgtggagga cccgggggag gttcccgccg ccgacgtcgc
     2821 ccatcggccc ggggtgaacg ttcgggaccg tgaagagcac gtcctcccca ctatcgcggg
     2881 agaagaagag cgaagcctgc tggacgcaga cctcctcgcc gatatcgcgg aagaacgcct
     2941 ccatgctctt tgagccgtcg gtcaggtggg cgatgaaggc gttgaggaag ttgagcccgc
     3001 tgacctggaa cgcccgtttc agcggccgct cgatcagccc gatcaggagg acgaagacca
     3061 gcccgaagac cacctggagg aggatggcgt aggggacgaa cccgggcgtg aatatccagg
     3121 caccgaccgc tatgccggtc acgccctgga ggagcgccgg ggcggccatc cgggtgatcc
     3181 ggtagtcggc cacggcgacg aggaccagca ggcggatgcc ggtggcaagc ccgaggacgc
     3241 ccgcgtagag cgcggaaaag agttcccgcc cgaagaccag gaccggcgag aggctcagta
     3301 tcaccatgag gaccgtgcag gcaagggcaa gaaggcccga ccggttgtag gtgatctgac
     3361 ggccaaagac ctccaccagc ggcacggtca gcaggaaggc gaccagggcc gggagcgcaa
     3421 agccgagcgt gccgaccagg aagaacccgt tgcccacccg gtatgcggct atatcgatga
     3481 cgaccccgag caggatgatg agcagaagcg accgcggcca ggacggagcg gagaagaggt
     3541 agcgggtgag ccgctcgaaa cgcacgtctg ggctggactc catcagatgc ccccgggagc
     3601 aggagccggc gataaagcgc cggcattcag cgcaatgcac cccatgattc agggagatat
     3661 ttcgcccggc tcaccaaaga ccttccgata ccggacaagc atccgctccc agtccggaag
     3721 gtttgtctgg gtgcccggcc gctcgacgac gaaggacgag accaccgctc ccgcgcggca
     3781 gcagtcgatc ggggcgtaac ccctccggta tgccgtgagg aacccggccc ggtaaccgtc
     3841 gccggcgccg gtggggtcga cggcattcac cctgacggcc gggacgtggt gctcctcgcc
     3901 gtccatgcag aggacgctcc cttccgctcc ccgggtcgtg acgacgatcg ggaccgaggc
     3961 gatgagcgcc gggcgttcca gccccagcat atcgcacatc ctgtccatct cgtggttgtt
     4021 cgagaagagt atatcgatct tcgcgagtat gatctcgagc tggtcggggg tgtagcggag
     4081 gaggtcctgg cccgggtcga acgatgcgaa ccggctcttt tcggccaccc ggacgttgaa
     4141 gtcggggtcg gccgtcgcca tgtggacgaa gtcgagcgcc ggggcctccg cccgggagaa
     4201 cgcggcggac gccccccact caaagaaggt ctcctggtcg ccggcctcgt ccgtgaagac
     4261 gtatgcggtc gccgtccggg cctccttcac cagggtgaag tcctgcacga taccgagatc
     4321 gcgcatccag cggtcgtagt cgctccccgg aaagtcgccg ccgaccggcg acaccagccg
     4381 gcaccgttcc ccgagcgtcg cgataccggc cgcgatgttc gccgccccgc cgccgaagta
     4441 gacctggtgg tcgacgatgt aggtcgagtt gtgccgcccg gggaggcccg ggacagagaa
     4501 gaggtggtcg atggcggtat gcccgacgac ggcgatcacg cctcctgcac ctcctggacc
     4561 tttaagggca cgtcgcgcag cgccttcccg acgaccgatt ttgcgatccg ggatgcgtgt
     4621 tcgccggact ccgcgcggaa gaccttcatc tggagaacga gccccacgag ggcggtgttc
     4681 gcgaccacga gcgcgctgtt gagttcaccc tcgcagtagg ggcaggcgat catacccgcc
     4741 tcgatatcca cgaacttcgc cgaggggttg agccgtttcc ccgcctcgct gatagcgatg
     4801 ctgacggcgt catccataga tttgacatcc ttgattatcc acgctgattc aagcgtaacc
     4861 agatagtccg gcatgagtct ctcctgttga taacctacca cgtctcgcgg tgcacgtgct
     4921 cctccaccgg catccgctcc acgggcctga gcggcggtat ccctttgccg accgcaagaa
     4981 gcgcaagggg gcggatgtgc tccgggagcc cgagggtctc gcggacgtcg ttctcatcga
     5041 acgctcccgt ccagcacgag tgcaggccgc gggcgtgggc ggcgagcatc atgtaggtgc
     5101 aggcgatcgt ggcgtcctcg agcgcgtaga ggatcccccg ctccccgtac tgcgacatgg
     5161 acctgacgta gtttgcgcag accacaaaga tcgccggagc ttcccggata tgcatctgct
     5221 cgagggccgc gaccgcaagc cctatccgca gatcctcgtc ggtgacgacg acgacgtccc
     5281 aggcctcgcg gttgccggcg ctcggcgccg tgctcgcgca cgtgaggatg tagtcgatat
     5341 cctccgggtc gagcggttct ccggtgtagt cccggaccga cgaccgcgcc ttgaggaggc
     5401 ggaggagatc agaggaggtc atgctctttg agcgtatccc acgccgtctg ggcggcggtc
     5461 gtgacggcgc tgacggctcg tgccatccgc tccgacgcgg cgccgagctc catgctctcg
     5521 gtcaggctga tcccctcgtt gaggtgatcg atggctttcc tgaactcacg ctgttccgtg
     5581 tcgccgtatg caaactcaag ctccgagcgg atagactgga gcaccatcag aagcatccgt
     5641 tttccgccgg gtttctcctg gagcggaaac tggatcaggg ctgtcgtcag ctgcgatccg
     5701 acgatcagct cggacttcgc ccgttcggca aactggtagg tcctgacggc ggttttgaga
     5761 tccataccac atactggaac tcgctactat aaaaagtatt gaaagagaag agagggagag
     5821 cttatctcga tttctgcttg gtcccgagag ccgaaacctt cgcgcgccgg atgctccggc
     5881 gtacccgcac gtccatcgtg ggagtgcccc cgcctctggc accgcgtccg ccacggccgc
     5941 cgcgtccacc gcgtccacca cgccgctggt cctgtccttc tctgcttgcc cgcttcctga
     6001 tatccgccag aggcttgaac cgctggttcg ggccgggttt ctttacttcg ccttcgcttg
     6061 cagggacgag ccgtatctgt cccttcacgc ccttaacctg ggcccacttt acgcttcctg
     6121 tctttcccat ggataaaact cctttacaat tatgagcgga tccgctgata aaagtaaccc
     6181 ggggccctgc cgcaacaggg caggaccccg ccgcagatca ggagaggaga cgcttgagct
     6241 cgcgccggag ggtctcgctg acgaccttgc cgtcgacgct ccccctgagt tcctgcatga
     6301 cgacgcccat aagggggccg agcgcgccca tgcctttttc gcgggcgaac gcctcgcgtt
     6361 cggccacaat ccggcgcacg atcgactcca cgtccgcctg cgagacggcg ggcgccatct
     6421 ttgcgatggc cgcatccacg cgttcgcggg gtgtcccggc gctctcgtcc gccgtccgcg
     6481 cgagttcggc gaggagatcc ggtatcgcct cctttgcggc ccggccggcc tccacggcgg
     6541 agaggagggc gaggatgtcg tcctcgctca cccggtcgat ggcgacgccg ccgcgggcaa
     6601 gctcgcggca ggtggcaagg agcgtccggg ccgcgagggt ggggcggacg ccggcggcga
     6661 cggccctctc gaagagcggc agccgttcgg agaacgccac ctgccgggcc agcgccccgt
     6721 caagcccgaa ctcccggacg aaccgctccg cacggtcggt gaggagttcg gggaccctga
     6781 tgccctccca gagggcgccg gagagcgcta tcgggaagac gtcggtctcg ggatacatcc
     6841 gggcggcccc ggggaggggg cgcatgtagg cggagctccc ctcctccagc atcttccggg
     6901 tctcttcggg aacgccggcg agggccatgt ccgcgcggat catcacctgt tcggcagcac
     6961 acccggcccg ctcccggctc gccgcaacga tggcgacgca gtcatcgtcg ccggcgccga
     7021 cgaactcgcg gaggcgcgcc acctcttcgg cggtgacgcc gtaggcgggg agttcgtcgg
     7081 tgtggaatat gccgccgaca ccgcacttct tcgcgtagtc ggacatctcg ctgccgaggc
     7141 ggcggccggg ctggatctcc cgcccgacga gccctgcaaa cccacagagc cggatcgcga
     7201 ggatggactt cgccttcttc agtacggcgg acttcgtccc ggcaaagagg gccgtgacgt
     7261 cgacgacggt gcggtccacc cgggcgcccc ggtcccggag ggcgtcgcgg atggcaagga
     7321 ggtttgtctg ccggtcgacc tcgcgcctga ccacctcggc gatgaggtcg agttcctgga
     7381 cgcccttgat ctcgacccgt gcgccgcctg ctatggagac gttgatgtcc tggcggatcg
     7441 tcccgaggcc ccgcttcacc ctgccggtcg accggagcac cataccgata tatgcggcga
     7501 cttcctggac ggcctcgggt gtgcgcatgc agggggcggt ggtgatctcg acgagcggta
     7561 tgccgagacg gtcgagggag aaggtctcgc cctccacccg ctgcgccgcc tcctcctcaa
     7621 ggcagatcgt ctcgatccgg cacccgtcgg ggagggcgcc ggagagagcg acgagcgccg
     7681 tccgctggaa cccgctggtg ttcgatccgt cgacgacgag ctttctcatc gtgtggatct
     7741 gttcgacggg ggtcatgtcg agcatcttcg ctatcgtgag gcagatgtcg agcgcctcgg
     7801 ggttcatcgg cgtcgggggc tcctcgtcgt ggtccacgag acagaccgtg tcgtaggtgt
     7861 agtagcagaa cttccggacg agtttcatct cctcttcggc cgcccggtcg atctccccga
     7921 gctcgctctc cgtcgcccgg aggtagcggt agaactcgcc cgtccggtcg gcaacatccc
     7981 ggaggagggt ggggcaccgg cagaagagtt tctcggcggt gtcgagctgc tggtggatct
     8041 cgatgccggc cttgaggccg aggtctttgt aatccattca gatcgacctc cgccggatct
     8101 cgcccgcgag atcggtctgc atcagcacgc cggcccgttc ggggtcgggc tcgttcccga
     8161 gcacccacat cagtttcacg agcgccacct cggggagcat atcctcgccc tcgatgacgc
     8221 cggcgcggag gaggtctctt ccggtgttgt agacccggtc gcagaccctc ccgtgcaggc
     8281 actgcgacgt catgacgacg gtcgtcccgc cctcgatcct atcccggagt ttcgggatcc
     8341 agtccgtcgc aacgtgcccg agtccggtcc cgacgatgac gaggcccgcg tagccctcga
     8401 aggcgtcgag gatgccgggc ggcatgccgg ggtagaactg gaggagggcg cagcgctcct
     8461 cgagtgtatc gtggagcccg ggctcttccg ccccccggcg caccgcctca tccgagaggg
     8521 tgaccgcgag cgaggggtag tcgacggaac cgagcggggc catgcccatg ctctggaaag
     8581 cgtcgcgccg ggatgtgtgc attttgcgga cccgcgtcgc ccggtggacg gcgcaccggt
     8641 cgtcgtttgt cgtcgcgtgc atcaccaccg ccacctcgcc gagatcgccg gcggcaacag
     8701 cggcgctgca gagggtgttc atggcgttgt cggagctcgg gcggtccgcc gaccgctgcg
     8761 acccgacgaa gacgaccggc accggcgtcc tgagcatgaa cctgaccgcc gcggccgagg
     8821 acgccatcgt gtcggtgccg tgggtgacga tgacgccgga cgtgccgtgc cggatctcgt
     8881 cgtagatcgc ccgggcaagg gcctgccaga ttgcgggacg catgttctcg gagaggatgc
     8941 ttgccacctg gcggtcgcgg tagcgggcga tatccccgag ttccgggatc gaccggagta
     9001 tgtcgtttgc cgagaactgg ctcgtcaccg ccccggtccg gtagtcgatc cggcttgcga
     9061 tcgtcccgcc ggtggatatg atggagagtt ccgggagggc ggggtcctgg atgacgatgc
     9121 cggccccggc cggcggctgc ggggctgcac gcccgacccg ttcaagcttc tcgggagatg
     9181 ccccgatgtt gtagccgctg tcgagcttga tgacggccat gccgtcccgc tcggcgatgt
     9241 agatgccggt gagtgcggtc ccgccgttgg cgtagcgcac ccggtctccg gcctcgagtg
     9301 attccgttat agtatcagcc tccgtgattc cgatatgaga tcttcaaaag cgcgagagag
     9361 cgccgtatcc gtctcctccg cccatgccct gtcccgggcg aggagttctt cctgctcagt
     9421 aagctgcacc gcgaccgccg cgggtgccgg gccgccgacg atcgtccgca ccgcgacggc
     9481 gtggcgcggg tcaagggcga gggcgatccg ctcctctgtc agcccgaggt cgacgaccga
     9541 taggccggcc gcttcgcggg cggctccttc gagcgtcgcg atgtcgagcg aaccgtgccg
     9601 gaccgcccgc ccgacgatcc ggtgggcggt gcggaaggga agtccgtact ccctgacgag
     9661 gacgtcggca agttccgtcg cggtcgagaa ccctctgccc gcctcggcgg ccatccgttc
     9721 ggtgttgaag gccgcggtgg cgatcatgcc ggacagcagg gggatgctct ggcgggcggc
     9781 ctccacaccc cgccagaggt ggggagtcag ttcctggagg tcgcggttgt agctcatggg
     9841 gagacccttc acgatggtga tcgccgcgat aagcgaaccg gcggccgacc cggccctggc
     9901 ccgcatgatc tccgccacgt cggggttctt cttctggggc atgatcgagg atgaggagca
     9961 gtaggcgtcg tcgaggtgga cgaacccgac gaacgcgctg ctccagatca ccagttcttc
    10021 gcagaaccgg cttgccgtcg tcatcagggc cgcgatatcc ccgagcacct cgattgcgaa
    10081 gtcccgggcg gcgaccgcgt ccatgctgtt tggcgccggc cgggagaagc cgaggagccg
    10141 ggcggtgtag tcgcggtcga gggggaagcc ggtcgaggcg aacgccgccg aaccgagggg
    10201 ggatacgtcc acccgggcgt aggcctcccg gagccgggcg gtatcgcggg agaaggcctg
    10261 ctcgtaggcg aggaggtggt gggcgagcgt cgtgggctgg gcatgctgca ggtgggtgaa
    10321 gcccggcatc accgtctccg tatgggcggc ggcaacgtcg aggagggtcc gccggaggcc
    10381 ggcgagcgat tggacgaggg cgatgagttc ctgcttcagg cgtatcctga tacaggtcgc
    10441 gacctcgtcg tttcgggacc ggcccatgtg gaggcggccg ccgaagtcct ccccggcccg
    10501 gccgatgagg taggcctctt tcccggcgtg gatgtcttcg tagtgctcat cgaacgcctc
    10561 ctccgggagg ccgtggtcgt ggaggtcgag gagcgccgcc atcagcgcac gggccggggc
    10621 ttcgtcgatg atcccctgcc gcgagaggcc gaggaggtgc gccatatcca cgaggaggtc
    10681 cgcctccgcg atccagcggt cggcggccat cgaggcgaga aaatgctcga ggtcggccga
    10741 acgctcgccg gaaagacggc cctgccgaat ctgatccgaa cgcatgctat caacagtcct
    10801 gtgtatcgtt tggcccggga agagattaaa accacgcccg gggtggggaa cgggcggcga
    10861 gccggggctc acgcgggagg aggacgccgg cggctgcggg ttggcggcag ggggcggcga
    10921 gccgggtcct gaaggcgtgg ggggtagact ggagaagagc cgggcacggc cgcccaccag
    10981 atatatgccc gggcactgca cggccccggg cgggggcggg ggaggaggcc ggaggcgtgg
    11041 gtggggtggg ggcggccggc ctcgccttcg cgctcttttg cggaggcgcc ccgcgccatc
    11101 tctcgtctcc gcgcacggcg gcagcgcccc ggccggcctc ctgcaccccc acctgatcat
    11161 ccttaaagga taattcatac attaaaatta atactgaagt ataacaataa accgattttt
    11221 cggtaacgct atatatcgcc agcatgaaga ttacacacat aggagatacc tatgagatcg
    11281 atcacagcaa ctcttctggt cgtggccatg gtcgcggcgt ttgcgtgcat cgccgggtgc
    11341 acgggcaccg gcgccgacga catgacgcac ctgcggatcg gctaccagcc gagcacccac
    11401 cagatcgctc acttcaccgc gatggagaag ggatggtggc aggaagacct cgcgcccctc
    11461 ggcatcacaa aggtgaccga caaccagttc ggcaccggcc cgcccgagat gcaggcaatg
    11521 cttgccggag aactcgatat cgcctacgtc ggcgcagcac cggtcgtatc cgccctggca
    11581 agcggccttg acgcaaagat catcgccgca gtgcagactc aaggatccga cctcatgctc
    11641 aggaacgaca tcgcatacga gagccctcag gacctcgtgg gcaaagagat cgccacctac
    11701 cacccgggct cgatccagga tacactcctc cggacctggc ttgagaagaa cggtgtcgac
    11761 cccgcgaagg tcatcatcaa acccatggac cccggtgcgg ccaccaccgc catcgctgcg
    11821 ggcaaggtcg acggcgtctt cctgcccgat ccctacccgg cgatcatcat agcagagggc
    11881 acggggagga ccatcgtgca gtccggcgag atgatgaagg accacgcctg ctgcgtcctc
    11941 gttgcaaccg gctcccttat ccgcgaacac cccgacatcg tcgaacagat tgtccggact
    12001 cacatcaagg cgacggagta caacttcgaa caccaggatg aggctgcaga gctctactcc
    12061 gcgaagaccg gacagaacct cgataccgtg aaggcatcct tcaagatctg ggacggcacc
    12121 tggaccgccg acccgcggat catcataccg tccacgatcg agtacacaaa cctccagtac
    12181 gagcttggct acatcaagaa gcccctgacc caggatgacc tcttcgatct ctcgttctac
    12241 gagaaggccc gggcataacc cgggaccaaa ccccaccatt ttccccccaa agtatctttt
    12301 gctgcgatgt cgataaagta ctgaagaata taccgggagg atcgatagac aattcctccc
    12361 ggcaggaata cctttgagca gcggaatcga gacatgaatc gacagacaca gaaacgactc
    12421 cttggcatca cagcgctcgt caccgtggcc gtgatctggc agatcatagc cgaatacgtg
    12481 gtagggcgat cgttcatcct cccgagcgtc accgatgtca tcagcgcatt cgtagatctc
    12541 atcgggtccg gcactctcct taccgatatc accgtaagcc ttgaacactt cgggatcggg
    12601 ctcctcgcgg cgttcttcct cggcgtcccg atagggatac tgatggggtg gttccgaacg
    12661 gcagacttcc tccttgaccc gatcatcgag gtcttccgcc ccattccgcc gcttgcctgg
    12721 ataccgttct ccatcatctg gttcggactc accacccagg cagcgggctt catgatcttc
    12781 gtcggagcct tcttccccat cctgaccgcg acatacaccg cgttccggtc cataccgaag
    12841 gtctttgtcg aggcgggcaa ggtgctcggg tgcaacacat cgtttgaact gatccgccac
    12901 gtcgccctcc ccgccgccat accggcgatc gcctccggca tcaggatagc gatgggcgtc
    12961 gggtggatgt gcctcgtagg tgccgagatg ttcggcgtat caaattacgg cctcggctac
    13021 cagctctggc acaactacta cctccaccag atgcccaacg tcgtcgtcta
//
LOCUS       DAOSEE010000056        12902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1881_length_12902_cov_250.351133, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000056 DAOSEE010000000
VERSION     DAOSEE010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 12902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12902
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1881_length_12902_cov_250.351133"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="PLI76_05780"
     CDS             complement(<1..893)
                     /locus_tag="PLI76_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOI61576.1"
                     /translation="MDLVYLAPIGAVLALLFAGYSYMSIKREGTGTELMQKIAAAIHE
                     GAMVYLNKQYRAIALFVIVLAIVLAIWIEPLTAACFVLGAALSATAGYIGMFTATAAN
                     VRTTNAARRGIADAFRVSFASGSVMGMAVVGLGLLGLSIAYMVISTFTGLTQAEVLTS
                     VTGFGLGASSIALFARVGGGIFTKAADVGADLVGKVEAGIPEDDPRNPAVIADNVGDN
                     VGDVAGMGADLYESYVGSIIAAMVLGVAVAATQFPNANIMNVIILPLLIAAVGIIASI
                     IGSFFVRTNKTESSAIHMAFN"
     gene            complement(984..2387)
                     /locus_tag="PLI76_05785"
     CDS             complement(984..2387)
                     /locus_tag="PLI76_05785"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase II small subunit"
                     /protein_id="HOI61577.1"
                     /translation="MLAEAEIVRRFLALKHQVHPDVVSYIREQNDPALIDRIAAGVPD
                     GTLVISAEHIPGLRKVRDGIRFLVDPELEVITGSAGSTGSVTGHEDAVHYFRDRYNNL
                     SSMIRGRMTTIPIEALVKSPHRYRDEECGIIGMVADARTTAKGHRLVEVEDPTGAIRV
                     LFNKGRDDSFLEAERILPDEVIGVKGKLSGDGGLLFAEHLFRPDIPINHAPFKSDRPG
                     KAVLISDVHVGSDTFLDEGWNRFADWLQDSDAAYLLIAGDLVDGIGIYPGQENELTIR
                     NIYEQYDVFAGMLRDLPSRIRIIAAPGNHDAVRMAEPQPAIPAEFSGKFPANCTLVEN
                     PCLLNLQGIRVLMYHGRSFDDMIGLIPGASYERAGEIMDEMLKRRLLASPYGMRTPIA
                     AMKSDRLVIDPLPEILLTGHVHICGVTRYRGVLGVNAGTWQSQTSFQKQMNIHPTPAR
                     AFVVDLQTLQPEVMDFS"
     gene            2490..3158
                     /locus_tag="PLI76_05790"
     CDS             2490..3158
                     /locus_tag="PLI76_05790"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOI61578.1"
                     /translation="MSDVTLLQRVASILAAFRESDNRVVSLARDLLWVVVVVGGIALA
                     LYLFAGTWPAVVTIESESMVPHMNVGDLVLVVDEDRFGGLTTWVEGQQTGHSSFGDYG
                     DVIVYRPNGADSVHPIIHRAMTYVDTAAVEESGLAQFYADPHGGYITKGDNNPYIDQG
                     NLRIAGIGLIEPVRKEWIVGKALVAVPLVGYLPLHLAEFAVIVVVIILIHDLILARRK
                     GKEE"
     gene            3188..4276
                     /gene="cofH"
                     /locus_tag="PLI76_05795"
     CDS             3188..4276
                     /gene="cofH"
                     /locus_tag="PLI76_05795"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HOI61579.1"
                     /translation="MPHSLHTLLCDTLAGHRLTEEEAVQLFTTRGRDIWKIAAAADEL
                     REERAGDAVTYVRNQNIHVTNICKNLCGFCGFGRRADDPGAFYDDPATVREKARTARE
                     RNVTEICLLSGVHPDYTLDSYADLISSVREAAPGVDIHTASPDEIVWAAAQSGTSTVA
                     AIERLREAGLGTLQGTAAEILVDSVRRIICPRKCDTATWVRVIREAHHLGIRSTATIM
                     YGHCESDADRARHLGILREIQDETHGFTEFIPLSFIHENTALYRAGLARAGATGREDI
                     LMVAVARLFLDNFDHVQVSWGKVGTKMAEAALLAGADDLGGTMFCDDVSTDAGSEGAD
                     YLDPRDMQRIADDIGRTLRQRTTTYGAV"
     gene            complement(4286..4603)
                     /locus_tag="PLI76_05800"
     CDS             complement(4286..4603)
                     /locus_tag="PLI76_05800"
                     /inference="COORDINATES: protein motif:HMM:NF020406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1805 domain-containing protein"
                     /protein_id="HOI61580.1"
                     /translation="MAQQILCHQRTRLSYKDAYGYVIPVGGTSLMTWVTDEGLIATAG
                     FDIEALANAGVPAAIVGPSEEESTLENLDDLMDAEVKAMNLPAIQRRIEVGMSGREAL
                     NRI"
     gene            4719..5810
                     /gene="cofH"
                     /locus_tag="PLI76_05805"
     CDS             4719..5810
                     /gene="cofH"
                     /locus_tag="PLI76_05805"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HOI61581.1"
                     /translation="MTPPLQELLSDTLAGHRLTEEEAVRLFATRDRDVWAIAAAADEL
                     REERVGDVVTYIRNQNINVTNLCVNACGFCGFSRKPGDPDVYRYDETVVRKKAREARE
                     RNVTEICTVSGLHPGFDAQSYIDIYSWIRDEAPAVHIHASNPMEVAYAARRSGLSTRE
                     VLIAMKAAGLSTLCGTAAEVLVDDVRRVICPDKIDTATWVRVIREAHYLGIPSTATIM
                     YGHCESDADRARHLGILREIQDETHGFTEFVPLSFIHENTPLYRAGLARAGATGREDL
                     LMFAVARLFLDNFDHVQASWVKLGTKMAEAALLAGADDLGGTLFEESISREAGARGTS
                     YLDPGDMRRMAEDLGRMLRQRTTTYDLLD"
     gene            5926..6081
                     /locus_tag="PLI76_05810"
     CDS             5926..6081
                     /locus_tag="PLI76_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HOI61582.1"
                     /translation="MADLISLAILFFILAIIFALLGVRGIAGMSMSIAKWLVIIFIIL
                     AILSLLF"
     gene            complement(6162..6713)
                     /locus_tag="PLI76_05815"
     CDS             complement(6162..6713)
                     /locus_tag="PLI76_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOI61583.1"
                     /translation="MSVLGISGSMRKNGNTALLVTTILDRVREAGIETEFLTLAGMDI
                     RPCTGCEACKSAKWCATEDDDWAGVAEKMIGCEVLVLGAPTYYYDINGQTKNLIDRTY
                     SLYHDRRLAGRKAVSVAVCADRGCERTLETIEGFLNTHEFSYLGYVCGKGYAPGDVRK
                     DERAMERARQVADTIIRYLRPED"
     gene            complement(6710..7165)
                     /locus_tag="PLI76_05820"
     CDS             complement(6710..7165)
                     /locus_tag="PLI76_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOI61584.1"
                     /translation="MDRGVYALILKNPHREIRIGALGRREFAEGSQIYVGSAHGSGGL
                     ARVERHIRLALRRDRSPRWHIDYLLLDPQFALVAAITAATDRDCECTLARAIGGSSAP
                     GFGCSDCTCPSHLFFRPTDPVEEVLTAFESLSLDARITRVKSEGNEHKV"
     gene            complement(7158..7760)
                     /locus_tag="PLI76_05825"
     CDS             complement(7158..7760)
                     /locus_tag="PLI76_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOI61585.1"
                     /translation="MPVRWIASGTTYANSFVYGNVLVDAGVLPMAVEPYAGAIDTIVL
                     THAHYDHTAHVKEIARLCGDAAVCIHEADAPGLVNDTRSLAMIFGARSPGIAPDVLLS
                     DGDPVGSLRVIHTPGHTPGGICLYDPAAKVLFSGDTVFAGGSFGRYDFPGGDRKALIA
                     SIERLAALDVEGLYPGHGEPAPTGGGRHIAAAREAFRFYG"
     gene            7786..9078
                     /gene="thiC"
                     /locus_tag="PLI76_05830"
     CDS             7786..9078
                     /gene="thiC"
                     /locus_tag="PLI76_05830"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOI61586.1"
                     /translation="MVLMHTLISACLRGVPPEVEAIAREEDLSPHHAARAVARGRIVV
                     PANPVRPHRLCAIGEGCRVRVNVNVGTSGIRCDEDLEVEKAKAALNEGADALMDLSTG
                     GDLVRIRRRILALDAPVGTVPVYEAVRRAGSAADLDADLLFSVVREHCRQGVDFLTLH
                     CGVNRDALASLEADPRTMGVVSRGGAFHVAMMAATGEENPLYAEYDYLLEILAEHDVV
                     VSLGDGMRPGAFVDAGRLAKTTEYLTLGHLAKRAIAAGVQRMIEGPGHIPADQIGYNV
                     RMIKELTDGAPLYLLGPLVTDVAPGYDHVVGAIGGAIACMHGADFLCMVSPAEHLALP
                     DIRDIVEGTRAARIAAHVGSLSRAAGSTRNHEARMAEARRALDWEKQFEAALFSGEAR
                     RIHERDGALETCSMCGDLCAVKMVREILKAPPEERMEP"
     gene            complement(9212..9604)
                     /gene="purE"
                     /locus_tag="PLI76_05835"
     CDS             complement(9212..9604)
                     /gene="purE"
                     /locus_tag="PLI76_05835"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOI61587.1"
                     /translation="MVDVAVICGSASDTPVAEKVFAVLQEHGISYDYQVISAHRDPER
                     LDGYVKASDARVFIAIAGLSAALPGVVASKTERPVIGVPVSGKLMGFDALLSIVQMPK
                     GVPVACVGVDNGENAGLLAVRILGAGRA"
     gene            9738..10868
                     /locus_tag="PLI76_05840"
     CDS             9738..10868
                     /locus_tag="PLI76_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HOI61588.1"
                     /translation="MEHEPLLMIPGPVPIPQRVRAAMTRQAINHRGPEFGAAYADTVR
                     TLKTLFGTAHDLYIISGSGSAGMEAAVANFARDRRIVSLVNGKFGERFAKIGERYGMV
                     ALLESEWGTPLDLAGLERELEAGAEIVTMVHNETSAGIKNPAPEVAKLARKHDALFIM
                     DGVTSIGGDDVRMDEWGVDIAVVGSQKCLAAPAGLAAIAVGDRAWDRICEKRPFYLDM
                     AAYRKSGSGTPMETPYTPAVPLFLALQEACTIIEEEGIPARIARHRRMADAVRAAAKG
                     WGVDLFPTLDARHAYSNTATAMRIPEGVTDKDLRGTVKKFGIEIAGGQDRLKGRIFRI
                     GTMGGIGAQEILATLTAVEFALKKSGFDAGAGVEAAAEVLLG"
     gene            10865..11326
                     /gene="ribC"
                     /locus_tag="PLI76_05845"
     CDS             10865..11326
                     /gene="ribC"
                     /locus_tag="PLI76_05845"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOI61589.1"
                     /translation="MKIGIADTTFARVDMGRIAADEIRKHASVGLERSVVPGIKDLPV
                     ACKKLIEERGCEIVMALGMPGGAEKDKICAHEASQGLILCQLMTNKHIIEVFVHEDEA
                     KDAKELAWLMERRTREHAANAVRLALYPKELEKLAGTGQRQGFEDVGPARP"
     gene            11385..11792
                     /gene="ribH"
                     /locus_tag="PLI76_05850"
     CDS             11385..11792
                     /gene="ribH"
                     /locus_tag="PLI76_05850"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOI61590.1"
                     /translation="MTVRLGFVVAEFNRDITYMMEIEAREHANFLGAEVAGAIYVPGA
                     YDMPLAIKKLLGQEDIDAVVTIGCVIEGATQHDEIVVQHAARKIIDLSLEFGKPVSLG
                     ISGPGMTRMEATERIDYARRAVESAVKMVQRLG"
     gene            11789..>12902
                     /locus_tag="PLI76_05855"
     CDS             11789..>12902
                     /locus_tag="PLI76_05855"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOI61591.1"
                     /translation="MRGLSEKIAGIPPSATIEISNAAKRMAQEGIDVISLSIGEPDFD
                     TPAHIKDACIDALRRGETHYAPSAGIPELTGAIAEKITRENGFSAKPDEVIVTCGAKD
                     AIYEAMEAVLNPGDEVLILDPSWVSYEPCARIAGGLARHHALSPETFQVDDTLLEAVG
                     PRVRMIVVNSPSNPSGAVLNADSLRLIADICRDNDIFALSDEIYEKLVYGKTPVSIAS
                     LPGMAERTITINGFSKAYAMTGWRLGYAVAPRPVIRQMEKVQQHTISHPTTFAMFGAV
                     AALEGPQDCVESMRREFERRRDYIIPALCDLGYTTAPADGAFYAYVNVGGDDMAIARS
                     WLHDAHVAVTPGTAFCTPGWLRLSYATSMENLMEAVG"
BASE COUNT         2352 a         4115 c         4175 g         2260 t
ORIGIN      
        1 ttgttgaagg ccatgtggat ggcgctgctc tcggtcttgt tcgtccggac gaagaacgaa
       61 ccgatgatgg atgcgatgat accgaccgcg gcgatgagca gcgggaggat gatcacgttc
      121 atgatattgg cgttcgggaa ctgcgtggcc gcgacggcaa cgccgaggac catcgccgcg
      181 atgatcgagc cgacgtagga ttcgtagagg tccgcgccca tgccggcgac gtcgccgacg
      241 ttgtcgccga cgttgtcggc gatgacggca ggattgcggg ggtcatcctc ggggatgccc
      301 gcttcgacct tgccgaccag atcggcaccg acgtctgcgg ccttggtgaa tataccgccg
      361 ccgacacggg cgaagagggc gatcgaactt gccccgagac cgaacccggt caccgaggtg
      421 aggacttcgg cctgggtaag gcctgtgaac gtgctgataa ccatatacgc gatcgagagc
      481 ccgagaagcc cgagcccgac gacagccatg cccatgaccg acccgctcgc aaacgagacc
      541 cggaacgcat ccgcgatccc tcgccgcgcg gcgttcgtgg tccggacgtt tgcagccgtg
      601 gcggtgaaca tgccgatata gccggccgtc gccgagagcg ccgcgccgag cacgaaacat
      661 gctgcggtga gcggttctat ccagatcgca aggacgatgg caagcacgat gacgaatagt
      721 gcaatagcgc gatactgttt gttcaggtag accattgctc cttcatgaat ggcggcagca
      781 atcttctgca tcagttcggt acccgtgccc tcccgtttga tactcatata cgagtatcct
      841 gcgaacagga gagcgagaac ggcacctatc ggtgccagat acaccagatc catctctttc
      901 tcattcctcc gacagagtac catatacaga ggaattcaac atttttaaat atgtggaaaa
      961 catccgggga aaaagggtga atatcagctg aaatccatca cttcaggctg aagtgtctgg
     1021 agatcgacga cgaacgcccg cgcgggtgta ggatggatat tcatctgttt ctgaaacgac
     1081 gtctgcgact gccaggtgcc ggcgttcacc ccgaggacgc cccggtagcg ggtgacgccg
     1141 cagatgtgga cgtgccccgt gaggaggatc tcggggagcg ggtcgatgac gagccgatcg
     1201 ctcttcattg cggcgatggg ggtccgcatg ccgtaggggg aggcaagcag ccggcgtttg
     1261 agcatctcat ccatgatctc cccggcccgc tcgtaacttg cgcccggtat gagcccgatc
     1321 atatcgtcaa acgaccggcc atgatacatg aggacccgga taccctggag gttcaagagg
     1381 caggggttct cgacgagagt gcagtttgcc ggaaatttcc ccgagaactc cgccggtatc
     1441 gcgggctggg gttcggccat cctgacggcg tcgtggttcc cgggagcggc aatgatccgt
     1501 atccgcgaag ggaggtcgcg gagcatgccg gcaaagacat cgtactgctc gtatatgttc
     1561 cggatcgtca gttcgttctc ctgtccgggg tagatgccga taccgtcgac gagatcgccc
     1621 gctatcagga ggtatgcggc gtccgagtcc tggagccagt cggcaaaccg gttccaccct
     1681 tcatcgagga atgtatcgct cccgacgtgc acgtcggata tcaggacggc tttccccggc
     1741 ctatcgctct tgaacggggc gtggttgatg gggatatccg ggcgaaagag gtgttcggca
     1801 aagagaagcc ccccgtcgcc cgagagtttc cccttcaccc cgatcacctc gtcggggagg
     1861 atccgctccg cctccagaaa ggagtcgtcc cgtcccttgt tgaagagcac ccgtatggcc
     1921 ccggtggggt cctccacctc gacgagccgg tgcccttttg cggtcgtccg ggcgtcggcc
     1981 accataccga tgatgccgca ctcctcgtcc cggtaacggt ggggggattt caccagcgcc
     2041 tcgatgggta tcgtcgtcat cctgccacgg atcatcgagg agaggttgtt gtagcggtcc
     2101 cggaagtagt ggacggcgtc ctcgtggccg gtgacgctcc cggtggaccc ggcgctcccc
     2161 gtgatcacct cgagttccgg atcgacgagg aaccggatcc catccctgac cttcctgagc
     2221 cccggtatat gctccgcgga tatgaccagg gtgccgtcgg gaacccccgc cgctatccgg
     2281 tcgatgagcg ccggatcgtt ctgttcccgg atgtaactca ccacgtcggg atggacctgg
     2341 tgtttcagcg caaggaaccg gcgtacgatc tccgcttcgg cgagcatgga ctcatgttat
     2401 tctccccggt ataatatggg tttatctctt ctccgcccgg tccccgggga acctatgtat
     2461 atgaagggag tgcactctct ttacaacaaa tgtcggatgt caccctgctg cagcgtgtag
     2521 catcgatcct cgcggcgttc cgggagagcg ataaccgggt cgtctccctc gcccgcgacc
     2581 tcctctgggt cgtcgtggtc gtgggcggta tcgctctcgc cctctacctc tttgccggca
     2641 cctggcctgc cgtggtcacg atcgagtcgg agagcatggt tccccacatg aacgtcggcg
     2701 acctggtcct cgtcgtcgat gaggaccggt tcggcgggct cacgacctgg gtcgaggggc
     2761 agcaaaccgg gcactcgtcg tttggggatt acggcgacgt gatcgtctac cgcccgaacg
     2821 gcgccgactc cgtccatccg atcattcacc gggcgatgac ctacgtcgat accgccgccg
     2881 tggaggagtc ggggcttgca cagttctacg ccgacccgca cggcggctac atcaccaaag
     2941 gggacaacaa cccctatatc gatcagggga acctccgcat agcaggcatc ggcctgatcg
     3001 agccggtcag gaaggagtgg atcgtcggaa aagccctcgt tgcagtgccg ctcgtgggct
     3061 acctccccct ccacctcgcg gagtttgccg tcatcgtcgt cgtcatcatc ctcatacacg
     3121 atctcatcct tgcgcggcgc aaagggaaag aggaataagt cccgggagat caatagagtt
     3181 gtttacgatg ccgcactcac tgcataccct gctttgcgat acgctcgccg gccaccgcct
     3241 caccgaggag gaggcggtgc agctgttcac aacccgcggc cgggatatct ggaagatcgc
     3301 cgccgccgcc gacgagctcc gggaggagag ggcaggcgac gccgtcacct acgtccgaaa
     3361 ccagaacatc cacgtcacca acatatgcaa gaacctctgc gggttctgcg gattcgggcg
     3421 gcgggctgat gatccgggcg cattttatga cgaccccgcg accgtgcggg agaaggcccg
     3481 gacggcgcgt gagaggaacg tcaccgagat ctgcctcctc tcgggggtgc accccgacta
     3541 cacgctcgac tcctatgccg acctcatctc ctccgtgcgg gaggccgccc ccggcgtgga
     3601 tatccacacc gcaagcccgg acgagatagt ctgggcggcg gcacagagcg gcacctcgac
     3661 cgttgcagcc atcgagcggc tccgggaagc gggtcttgga accctgcagg ggacggcggc
     3721 cgagatcctg gtggacagcg tccggcgaat catctgcccg aggaaatgcg atacggcgac
     3781 atgggtccgg gtcatccggg aggcgcacca tctcgggatc cgatcgaccg cgacgatcat
     3841 gtacggccac tgcgagagcg atgcggaccg cgcccggcat ctcggcatcc tccgcgagat
     3901 ccaggacgag acccatgggt tcaccgagtt catccccctc tcgttcatcc acgagaatac
     3961 cgctctctac cgtgcgggtc tcgcgcgagc gggggcgacg gggagagagg atatcctgat
     4021 ggtcgctgtc gcccgcctct tcctcgacaa cttcgaccac gttcaggtct cctgggggaa
     4081 ggtgggaacg aagatggcgg aagcggcgct tcttgccggt gccgacgacc tcggggggac
     4141 gatgttctgc gacgacgtct cgaccgacgc cggcagcgag ggcgccgact atctggaccc
     4201 gcgcgatatg cagcggatcg ctgacgatat cggccggacg ctccggcagc ggacgacgac
     4261 ctacggggcg gtgtgaggcg acccctcaaa tcctgttcag ggcctcgcgc cccgacatac
     4321 cgacctcgat gcggcgctgt atcgccggaa ggttcatcgc cttcacctcg gcgtccatca
     4381 ggtcgtcgag attctccagg gtggattctt cctccgaggg gcccacgatc gccgcgggaa
     4441 ctccggcatt tgccagcgcc tcgatatcga acccggcagt cgcgatcagc ccttcatcgg
     4501 tcacccaggt catcaggctg gtcccgccga ccgggatgac gtagccgtat gcgtccttgt
     4561 agctgagccg tgtccgctgg tgacatagga tctgctgagc catggctgcg gagatgttcc
     4621 cgggacagat aagtatatgc cctccgccgg gagcgaatcc gaaggttctt gaactgaccg
     4681 cgccaaatgg gatcatcctg taaagatggt cgaggtgtat gacgccaccg ctgcaagagc
     4741 tgctcagcga tacgcttgcc ggccaccggc tcacggagga ggaggccgtc cgcctcttcg
     4801 cgaccaggga ccgggatgtc tgggcgatcg ccgctgccgc cgatgaactc cgggaagaga
     4861 gagtaggtga cgtcgtcacc tacatccgga accagaacat caacgtgacc aacctctgcg
     4921 tgaacgcctg cgggttctgc gggttctctc gaaaaccagg cgatcccgac gtatatcgct
     4981 acgacgagac cgtcgtgcga aagaaggccc gggaggcgcg cgagcggaac gtgaccgaga
     5041 tctgcacggt gagcggcctc cacccggggt ttgacgccca atcctacatc gatatctact
     5101 cctggatacg ggacgaagcg cccgctgtcc acatccacgc gagcaacccg atggaggtgg
     5161 catacgccgc gaggaggagc ggtctctcca cccgggaggt gctcatcgcg atgaaagcgg
     5221 ccggcctctc gacactctgc gggacggcgg ccgaggtcct ggtggacgac gtccggagag
     5281 tgatctgccc tgacaagatc gatacggcga cgtgggtccg ggtcatccgg gaggcgcatt
     5341 accttggtat cccatcgacc gcgacgatca tgtacggcca ctgcgagagc gatgcggacc
     5401 gcgcccggca cctcggcatc ctccgtgaga tccaggacga gacccatggg ttcaccgagt
     5461 tcgtccccct ctcgttcatc cacgagaata ctcctctcta ccgggcgggt cttgcacgag
     5521 ctggggcgac ggggagggag gatctcctta tgtttgcggt cgcccgtctc ttcctcgaca
     5581 acttcgacca cgtccaggcg tcctgggtga agctcgggac gaagatggcg gaggcggcgc
     5641 ttcttgccgg cgccgacgac ctcgggggga cgctctttga ggagagcata tcccgggaag
     5701 cgggcgcgcg gggcaccagc tacctggacc cgggcgatat gcggcggatg gccgaagatc
     5761 tcggccggat gctccggcag cggacgacaa cctacgacct cctggactga tatcgcctga
     5821 ggatcggatc ttctcccgat ccaccgctat tctcggaaag atatatatgc ccacggatgg
     5881 agtgtggctc ctcaacgccg actggcgatt gggagggaga cagatatggc agatcttatc
     5941 agccttgcaa tcctcttctt tatactggcg attatctttg ccctgctcgg ggtaaggggc
     6001 atcgctggaa tgtcgatgtc gatcgcgaag tggctggtca tcatcttcat catcctcgcg
     6061 atactatcgc tgctcttctg accgcgggaa gggcgttcgg gctgctgggc ccggagatcc
     6121 ttcccgcggt cagggatgcc ccggccgggc gtatccgctc ttcagtcctc cggtcggaga
     6181 tacctgatga tcgtatccgc aacctgccgc gccctctcca tcgcgcgctc atccttccgc
     6241 acatcccccg gcgcatagcc ctttccgcag acgtaaccga ggtaggagaa ctcgtgggtg
     6301 ttgaggaacc cctctatggt ctcgagcgtc cgttcacacc cccgatcggc gcagacggcg
     6361 acggaaaccg cttttctgcc cgcaagacgc ctgtcatggt agagggagta ggtccggtcg
     6421 atcaggttct ttgtctgccc gttgatgtcg tagtaatagg tcggggcgcc gaggaccagg
     6481 acttcgcagc cgatcatctt ctctgccacg ccggcccagt cgtcgtcctc cgtcgcgcac
     6541 cacttcgcgc ttttgcaggc ctcgcagccg gtgcagggac ggatatccat accggcgagc
     6601 gtcaggaact cggtctcgat gcctgcttcc cgcacccggt cgaggatggt ggtgacaagg
     6661 agtgcggtgt tgccgttctt ccgcatgctt ccggatatgc caagaacact cataccttat
     6721 gctcgttccc ctcgctcttg actcttgtga tccgggcatc cagggagagg ctctcgaacg
     6781 cggtgaggac ctcctcgacc ggatcggtgg ggcgaaagaa gaggtgcgag gggcaggtgc
     6841 agtcgctgca cccaaatccc ggagcagaag acccgccgat ggcacgggcg agggtgcatt
     6901 cgcagtcccg gtcggtcgcg gcggtgatgg cggcgacgag agcgaactgc gggtcgagga
     6961 ggaggtagtc gatgtgccac cggggggagc ggtcacggcg aagggcgagc cggatgtgcc
     7021 gctccacccg ggcaaggccg ccgcttccgt gggcggagcc cacgtagatc tgcgagcctt
     7081 ccgcgaactc tcttctcccg agagcgccta tgcggatctc ccggtgcggg ttcttgagga
     7141 tgagggcgta gacccctcta tccatagaac cggaacgcct cccgggcggc cgcgatatgc
     7201 ctcccgccgc ccgtgggggc aggctcgccg tgcccggggt agagcccctc gacatcgagt
     7261 gctgcgagcc gttcaatcga cgctataagt gccttccggt cgccgccggg gaagtcgtag
     7321 cgcccgaacg atccgccggc gaagacggtg tcccccgaga agagcacttt tgccgccgga
     7381 tcgtagaggc agatcccgcc cggcgtgtgc cccggcgtgt ggatgacgcg gaggctcccg
     7441 accgggtcgc cgtcggagag gaggacgtcg ggggcgatcc cgggcgatcg ggcgccaaag
     7501 atcatggcga ggctccgggt gtcgttgaca aggccgggag cgtccgcctc gtggatgcag
     7561 acggcggcgt cgccgcagag ccgggcgatc tccttcacgt gggcggtgtg gtcgtagtgg
     7621 gcgtgggtga ggacgatcgt atcgatagcg ccggcatacg gctccaccgc catcgggaga
     7681 acgcctgcat ccacgaggac gtttccgtaa acgaacgagt tggcgtaagt cgtgccgctc
     7741 gcgatccacc ggactggcat gtagactaat atggcctccg gacaaatggt tcttatgcat
     7801 accctgatct ccgcatgcct gcgcggggtc cccccggagg tggaggcgat cgcccgcgag
     7861 gaggatcttt ccccgcacca tgccgcgcgg gccgtcgccc gcgggcggat cgtcgtcccg
     7921 gcaaaccctg tgcggccgca ccggctctgc gccatcggcg agggctgcag ggttcgggtc
     7981 aacgtgaacg tcgggacatc gggtatccgg tgcgacgagg acctcgaggt cgagaaggcg
     8041 aaggcggccc tcaacgaggg tgcggatgct ctgatggacc tctcgaccgg cggcgatctc
     8101 gttcgcatcc ggcgccggat cctcgccctc gacgcgcccg tcgggacggt cccggtgtat
     8161 gaagcggtcc ggcgggcggg gagcgcagcg gatctcgatg ccgacctgct tttctcggtg
     8221 gtccgggagc actgccggca gggcgtggac ttcctgacgc ttcactgcgg cgtgaaccgc
     8281 gacgcgctcg cctccctcga ggcggatccc cggacgatgg gcgtcgtctc ccgcggcggg
     8341 gccttccacg tggctatgat ggccgcgacc ggtgaagaga accccctcta cgccgagtac
     8401 gactacctgc ttgagatcct tgccgagcac gacgtggtcg tgagcctcgg ggacgggatg
     8461 cgccccggcg cgttcgtgga tgccggccgc ctcgcaaaga cgaccgagta cctgacgctc
     8521 gggcatctcg cgaagagggc gattgccgcc ggggtgcaac ggatgatcga ggggccgggg
     8581 catataccgg ccgaccagat cggctacaac gtccggatga tcaaggagct gaccgacggc
     8641 gcaccgctct acctgctcgg cccgctcgtg accgacgtgg cgccgggcta cgaccacgtc
     8701 gtgggggcga tcggcggcgc gatcgcctgc atgcacggcg ccgactttct ctgcatggtc
     8761 tcgccggccg aacatctggc gctgccggat atccgcgaca tcgtggaagg gacgcgggcg
     8821 gcaaggatcg ccgcacacgt cgggagcctc tcccgcgccg ccgggagcac caggaaccac
     8881 gaagcccgta tggcggaggc gcgccgggcg ctcgactggg agaaacagtt cgaggcggcg
     8941 cttttctccg gggaggcccg gcgcattcac gagcgggacg gggcgcttga gacctgctcg
     9001 atgtgcggcg acctctgcgc cgtaaagatg gtccgggaga tcctcaaggc gccgccagaa
     9061 gagcggatgg aaccgtaaga ggcgttcgct gccggcacta cggggcgccc ttccctccga
     9121 actgcccgtc gatccaggcg gttatcgcgc tccggctccg ccggtaggcc gcgaggaccc
     9181 tgtcaggtcc gggaagtcgg catggagcgt atcaggcgcg gcccgcgccg aggatccgca
     9241 ccgcgagcag gccggcattc tcaccgttgt ccacgccgac gcaggccacc gggacgcctt
     9301 tcggcatctg gacgatcgag aggagggcat caaaccccat cagtttcccg ctcacgggga
     9361 caccgatcac cgggcgttcc gtcttcgagg cgaccacgcc cgggagggcc gcggaaagac
     9421 cggcgatagc gatgaagacc cgggcgtcgc ttgccttcac gtacccgtcc agcctctcgg
     9481 gatcccggtg cgccgagatc acctggtagt cgtaggatat gccgtgctcc tgtaagactg
     9541 caaacacctt ctccgctacg ggtgtatccg aagcggagcc gcagatcacc gcgacatcaa
     9601 ccatacctcc ctctggtatg ctcctgcccc ttcatctctt taccgatatc gcaccgtggt
     9661 gaggttgata agagtttaca tccaagttcc ttcaggaact gcgtccgtcc ccgacgagac
     9721 gatacggact gatcaccatg gaacacgaac cactcctcat gattcccggc cccgtgccca
     9781 tcccgcaacg ggtacgcgcc gccatgacgc ggcaggccat caaccatcgc ggccccgagt
     9841 tcggcgccgc atacgcggat accgtccgga ccttaaagac cctttttggc accgcacacg
     9901 acctctacat catcagcggc tccgggagcg ccggtatgga ggccgccgtc gcaaactttg
     9961 cacgggacag acggatcgtc tcgctcgtca acggtaagtt cggcgaacgt tttgcaaaga
    10021 taggtgagcg ttacggtatg gtcgccctcc tcgagtccga gtgggggacg cccctcgatc
    10081 ttgcaggtct tgagcgggaa ctcgaggccg gggccgagat cgtcacgatg gtccacaacg
    10141 agacgagcgc cggtatcaag aaccccgcgc ccgaggtcgc gaaactcgcc aggaagcacg
    10201 acgccctctt catcatggac ggtgtcacct ccatcggcgg cgacgacgtc cgcatggacg
    10261 agtggggcgt cgatatcgcc gtcgtcgggt cgcagaagtg cctcgcagcc cccgcgggcc
    10321 ttgccgccat cgccgtcggc gatcgcgcct gggaccggat atgcgaaaag cgtcccttct
    10381 acctcgatat ggcggcctac cggaagagcg gaagcggcac cccgatggag accccctaca
    10441 ccccggcggt cccgctcttc cttgccctcc aggaggcctg cacgatcatc gaggaagaag
    10501 gcatcccggc ccggattgcc cgccaccgcc ggatggccga cgccgtccgc gccgcggcga
    10561 agggatgggg cgtcgacctc ttcccgacgc tcgatgcacg ccacgcctac tcgaacaccg
    10621 cgaccgcgat gcggatcccc gaaggcgtca ccgacaaaga tctccgggga acggtcaaga
    10681 agttcggtat cgagatcgcc ggcggtcagg accgcctcaa gggcaggatc ttccggatcg
    10741 gcaccatggg cggcatcggg gcgcaggaga tccttgcaac cctcacggcc gtcgagtttg
    10801 ccctcaaaaa gtccggattc gatgccggcg ccggcgtcga ggcggccgcc gaggtgctgc
    10861 tcggatgaag atcgggatcg ccgacaccac gtttgcccgg gtggatatgg gcaggatcgc
    10921 cgccgacgag atccggaaac acgcaagcgt ggggctcgag cgctccgtcg tcccgggcat
    10981 caaggatctc ccggtggcgt gcaagaagct gatcgaggag cggggctgcg agatcgtgat
    11041 ggccctcggc atgcccggcg gggccgagaa ggataagatc tgcgcccacg aggcctcgca
    11101 gggcctcatc ctctgccagc tcatgacgaa caaacacatc atcgaggtct tcgtccacga
    11161 ggacgaggcg aaggatgcaa aagagcttgc ctggctgatg gagcggcgga cgagggagca
    11221 cgcggcgaac gccgtccggc tcgccctcta tccgaaggag cttgagaagc tcgccgggac
    11281 cggtcagcgg caggggttcg aggacgtcgg gcccgcacgc ccctgacgta aagagcgatg
    11341 gttatcagta tccggtgcga ttactaccac agggattgat tgatatgacg gtaagacttg
    11401 gatttgtcgt cgcggagttc aaccgcgaca tcacctacat gatggagatc gaggcccggg
    11461 aacacgcgaa cttcctcggc gcggaggtgg cgggagcgat ctacgtcccc ggcgcctacg
    11521 acatgccgct cgcgatcaag aaactgctcg ggcaggaaga catcgatgcg gtcgtcacga
    11581 tcgggtgcgt catcgagggc gccacccagc acgacgagat cgtcgtccag cacgcggcgc
    11641 gtaagatcat cgacctctcg cttgagttcg ggaaacccgt ctctctcggc atatccgggc
    11701 cggggatgac ccggatggaa gctaccgaac gtatcgacta cgccaggcgt gccgtcgagt
    11761 cggccgtgaa gatggtccag cgattgggat gagaggcctc tcagagaaga ttgcgggcat
    11821 ccctccctcc gcgaccatcg agatatcgaa cgcggcaaag cggatggcgc aggaaggtat
    11881 cgatgtcatc agcctctcga tcggggagcc ggacttcgat accccggcac acatcaaaga
    11941 tgcctgtatc gacgctctcc gccgcggaga gacgcattac gccccgagtg ccggcatacc
    12001 ggagctgacg ggcgcgattg ccgagaagat cacccgcgag aacgggtttt ctgcaaagcc
    12061 ggatgaggtg atcgtcacct gcggggcaaa agacgccata tacgaggcga tggaggccgt
    12121 cctgaacccc ggcgacgagg tgctcatcct cgatccctcc tgggtctcct acgagccctg
    12181 tgcccgtata gcgggcggcc ttgcccggca ccacgcgctc tctcccgaga cgttccaggt
    12241 cgacgatacc ctgcttgagg ctgtcggccc ccgggtaagg atgatcgtgg tcaactcccc
    12301 ctcgaacccc tccggcgcgg tgctgaacgc ggactctctc cggctgatcg ccgatatctg
    12361 ccgggacaac gatatattcg cactctccga cgagatctac gagaaactgg tctacgggaa
    12421 gacgccggta tcgatcgcct ccctcccggg tatggcggag cggacgatca ccatcaacgg
    12481 gttctcgaag gcctacgcga tgacggggtg gcggctcggc tacgcggtcg cgccccggcc
    12541 ggtcatccgg cagatggaga aggtgcagca gcacaccata tcgcacccga cgacgtttgc
    12601 catgttcggc gcggtcgccg cgcttgaggg gccccaggac tgcgttgaat cgatgcgccg
    12661 cgagttcgag cgccggcgcg actacatcat accggcgctc tgcgacctcg gctacaccac
    12721 cgctccggcg gacggcgcct tctacgccta cgtgaacgtc ggcggcgacg acatggcgat
    12781 cgcccggtcg tggctccacg acgcccacgt ggcggtcacc ccggggacgg cgttctgcac
    12841 ccccggctgg ctccggctct cctacgcgac gtcgatggag aacctgatgg aagccgtcgg
    12901 gc
//
LOCUS       DAOSEE010000057        12853 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1888_length_12853_cov_209.441163, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000057 DAOSEE010000000
VERSION     DAOSEE010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 12853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12853
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1888_length_12853_cov_209.441163"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..1353)
                     /locus_tag="PLI76_05860"
     CDS             complement(154..1353)
                     /locus_tag="PLI76_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI61592.1"
                     /translation="MNERHQMPDGHAAYTLFAIGFFAIFSTTIAKNPVLPLFSQALGA
                     GDAVVGLVAAVSPLAGILFSFPVGVLSDHLGRRRLLIASGAVFLSAPLLYLLITDPFW
                     LIPIRFFHGTATAILGPVIAATIVVRFAGRKGEVLGQYSSATLVGRTLAPLVGGAIIS
                     FFAVYPGLVPYRAVYIAAAVAAVPVLALILLYLEETPAPLTVLSFSAFRMSSVAFFAN
                     RRLRATALADMATYFAFGAFETFLPLVLLSQGMGAYQAGVLFAAQTLIIAATKPSFGR
                     LSDRVDRRIQIVAGLLILGCAVAAIPFAPGFFAFLLVSALFALGMSLSTVATSAYVAD
                     VAEQEQMGASMGALSSIMDIGHSAGPLVTGIIVAAGGFGPGFFASFLIAILICGFFVL
                     SVREPAG"
     gene            2105..4147
                     /locus_tag="PLI76_05865"
     CDS             2105..4147
                     /locus_tag="PLI76_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOI61593.1"
                     /translation="MAKRMLSEFESYDLLKQYGVPVPDHVVVKTAAEAGKAAEKIGFP
                     VVMKIYSPQIVHKSDAGGVIVSIPSKSAAEEAFEKIVKSAKAYNPEAEIKGVIVEQQA
                     APGLELIIGGKTDPAFGKVLTFGMGGTLVELMRDVTLRILPITEETIREMIQEIHGYP
                     MIKGYRGTKPRDEEALVQIMWAVNCFFAENTHVVEFDINPIRLYESGACIVDARIFVD
                     DEVVEKVAKERPFVPIEYFTPRSIAVIGASSEPKKMGYAVMHNLLHFPGQLYPVNNKR
                     PEVQGLKAYASILDIPNPVDMAVITVPAKHVPSVIEECGQKGVSMAVIITAGFKETGE
                     GGKALEDRILAIAKGYGTRIVGPNCLGLIIPPKGIDTTYVHESPKPGNIAFISQSGAI
                     VNTVVDWSIKHDIGFSAVVSVGNQADLNFIDFLRFVERDPKTKGIILYIEEIQDGKTF
                     MKVAGEVARTKPIVAIKSGSSEKGQAAASSHTGSLSGSYDVYMEAFRNAGVIPVHTLT
                     GTFQVAEMLSLPKGYPRGKRAVVVTNAGGFAVLSSDYAERYGIDLISLPPAVLKELND
                     LLPDFWNKNNPIDLLGDANEKRFEQTFNVLARHQDCWDIAFVVGFPNLVIGSDQLANQ
                     IIKFSEKTENMIVATLLGGGSMDRGRKILKENGIPLFEELDFTFRVMGRILWQRFR"
     gene            complement(4415..5962)
                     /locus_tag="PLI76_05870"
     CDS             complement(4415..5962)
                     /locus_tag="PLI76_05870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOI61594.1"
                     /translation="MKTSARISFLTATLVVLLVLVLSRDILLILAGAGWSPAARALSL
                     LLIVVTAFGVIQFTNYADHLLRSLYVYPEHPLAPRQETAPPAGRVAVFIPVYNEEPGV
                     VESCIRACTSIAYPDCTVYLLDDSTDERLRGSMEEIGRRYRIRYVHRDHRKGFKAGAI
                     NHALSLLDGDTQYLLVLDADQIVKPAILADLIPVIEADPTLSFIQTPQFFRPDPDNLI
                     SATFSYQQHIYNKHVCRGLSINRTTMLTGSNCIFRVGHLLAIGGMDETTITEDIATAF
                     LFHTGGYRGVYIDAVYAEGMAPPDLAAYFTQQLRWAYGNTRLLGKVMRQFVAHPRSLA
                     PVQWLEFAVIASVYLFGGLSVVFFLLPVATLLFGIPILPIWLPVVFAVVFGTVIAIQV
                     VTSIRERHYSPRDLAFSQAIFNTLSLVYIRAIWYAVSGKKLPFVVTPKTAFVSGERAR
                     VRITPLLAVIAAILLSMLAGIVRLLTRGPDFGTIVPLFWAGFTLLVLSSFIVVWRKEG
                     KAGARGS"
     gene            6309..6926
                     /locus_tag="PLI76_05875"
     CDS             6309..6926
                     /locus_tag="PLI76_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOI61595.1"
                     /translation="MATPSLRKYELPPLPYAPDALEPYISREQLTLHHDKHHQAYVTG
                     ANADLEKLEKARQEGTEVDMKALLKELSFNVGGQVLHTLFWPNMAPAGSGGGGKPGGA
                     LADRIDREWGSFDRFKAEFTKAAASTEGSGWAALSHCSLTDRLMIMQIEKHNNNVYPM
                     FGILMVLDVWEHAYYVDYKNNRGQFIDAFWNVVNWDTVNKRLENL"
     gene            7275..9203
                     /locus_tag="PLI76_05880"
     CDS             7275..9203
                     /locus_tag="PLI76_05880"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOI61596.1"
                     /translation="MGRVNPVFVRPEETFEILDEPGVGILVLDRDLRVAWANARAMDI
                     LPIGNDIPGADARRVLGALLQEEESVRKLLDGASDISGIDLPARGPRGGKRRIRYSSR
                     KIEQGPFAGIRVLRLRDVAGEERYRSFFRDFQGIAYRTTESGPVLFHGAVEAITGYTQ
                     DDFLEGAVCWDAVVHPDDRPGFQECAGELRAVPGLSTERVYRIVRKDGTTRWVRDFAK
                     NVCNEDGTPALVQGTIHDAIGRREADDGLLSSNRQLSVLNKIMGVAASSLSLDELLEA
                     SLSVTIDLLDLDVGLTYLLNPERTLALTRYHHAAPKRYLARSRTIKVHHWPWNFVFVA
                     GQPRYIERDGNPGAAEEEILASLEVSALACIPILAESVVVGALFLGRRDRPRIRDEER
                     RLLEAIGREIGSGVLRSMLHKRLEAAHRETNLYLDVMTHDLKNAGNVANLYCDLLIDE
                     PGGAAAEYAKRLRESIRKSTGILRNVSTIRRIHQEPPDLRPVGLDRVICAERERMPEA
                     AVTLEGTPAEVWADDLLSEIFANLIGNAVTFGGPGAGITIRVEGCAKENRMVVVTVED
                     TGPGIPDGVKEALIQRLGGDEDQVHGEGLGLSVVGMLVERYGGRVWIEDRVSGRPDLG
                     AAFRFTLREVARTERKPT"
     gene            complement(9220..10635)
                     /locus_tag="PLI76_05885"
     CDS             complement(9220..10635)
                     /locus_tag="PLI76_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOI61597.1"
                     /translation="MPTSVPKDDTHSHNNQNETEGTKLLLADPKTAILRLSLPVMVAM
                     TLMTLYSVVDAFWVAGLGADALAAVGFSFPLFIITIGLASGLGTGGEAALARMIGAGD
                     RDGASSVAMHTILLMTGLAVAVTIPLALFAGDIFALMGAGSAAGLAVAYARILFMGTF
                     ALLFGEVAYALMHGEGDAKRAMYAMAAGAVANIVLDPILIYTCGMGIEGAAVATIIAE
                     IVAAVPMVYWLFVKRDTYISLSLRRFAPDPAVARDILQVGLPAAAEQLSLALMTLVLN
                     GIIVLISSTDGVAVYSTGWRVVSIGLTPILAIATSVVAVTGASYGARAYARMESALRF
                     AIRLGTGIGIGLAAATFILAPGIAALFATPEITPELTSYLRVMSLVYPMVGFGLFSAS
                     FFQGTGLGARSLTITVLQNVVLSTLLAWMFAIDLGFGLSGVWWGVAAGNAIGALLGYA
                     WARRYTAGLRVRQAGAAKAAA"
     gene            complement(10895..11152)
                     /locus_tag="PLI76_05890"
     CDS             complement(10895..11152)
                     /locus_tag="PLI76_05890"
                     /inference="COORDINATES: protein motif:HMM:NF015442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOI61598.1"
                     /translation="MVKADGRKEPFAREKVVVSALKAGAPLEEARAIGEAVERDAYDG
                     MPAADIRQRVLSHLRDRNPEWEENWLMYDRAVKRREVLAAR"
     gene            complement(11198..12286)
                     /locus_tag="PLI76_05895"
     CDS             complement(11198..12286)
                     /locus_tag="PLI76_05895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOI61599.1"
                     /translation="MQDRCDVLVVGAGPAGSTAARYAAEAELDVLLIDKRREVGLPVC
                     CGEFNASSEVLRTALPAASVPDEPDLPRRAIRGAVAVSPGGREYGFDLPGYTLDRDAL
                     DRSLADAAVRAGAALSLETTFRGRDGRKIITDRGTITAGVVVAADGPLSLVSRSAGME
                     RCAVLAPAVTCRVDGEFGDRIRLYFGNSVAPGGYAWVFPKEGCANVGLGVQGRTTSVT
                     LLLRAFLARMGFIGRDVRAGFVPVAGPIPETVRENVIAVGDAAGQVVASNGGGIATAM
                     ICGRLAGIAAARHLLHDEPLRAYERAWQSAMGRELGTAKKTKRMADWVLGSDFLIERA
                     MAALGDRGLRNVIVYGGLTPPRRRPERL"
     gene            complement(12332..12565)
                     /locus_tag="PLI76_05900"
     CDS             complement(12332..12565)
                     /locus_tag="PLI76_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3006 domain-containing protein"
                     /protein_id="HOI61600.1"
                     /translation="MQDESTFRASLDRVEEGLAVLLIREDESIRFTLPRSLLPADARE
                     GDILDVTVRRDTAATKEARRRVAERIERLRSRG"
     gene            complement(12570..>12853)
                     /locus_tag="PLI76_05905"
     CDS             complement(12570..>12853)
                     /locus_tag="PLI76_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOI61601.1"
                     /translation="YLSLLDEMVTVTNAGATAVNLTGWTIADEGARNTYTFPVFVLDA
                     GSNVTVHSGAGNETASDLYWGRSSPVWNNDGDTATLADASGAIVSVLEG"
BASE COUNT         2503 a         4053 c         4236 g         2061 t
ORIGIN      
        1 cggttgatac ctacgtgtag atgtagtagg gcaatcctta aacctttcga atttgacaat
       61 gccgcgcgga ggcacgcttg aggattatcg aggtgccggg cggccgggaa ttcccaccgg
      121 cggggcaggc gagggtatct gccgcgctcg ccgttatcct gccggctccc ggacggagag
      181 aacaaagaac ccacagatca ggatggcgat caggaaactc gcgaagaacc cggggccaaa
      241 cccgcctgcg gcaacgatga taccggtcac gagggggccg gccgagtgcc cgatgtccat
      301 gatggacgag agcgccccca tcgaagcgcc catctgctcc tgctccgcaa catccgcgac
      361 gtacgcgctc gtcgcgaccg tggagagcga cataccgagg gcaaagaggg cgcttacgag
      421 gaggaacgcg aaaaatcccg gcgcaaacgg tatggcggcg accgcacacc cgaggatgag
      481 gaggccggcg acgatctgga tgcgcctgtc gacccggtcg gagagcctgc caaacgaggg
      541 ttttgtcgcc gcgatgatca gcgtctgagc ggcaaacagg actcccgcct ggtacgcgcc
      601 catcccctgc gagagcagca cgagcggcag gaacgtctcg aacgccccga aggcaaagta
      661 ggtcgccata tccgcgagtg ccgtcgcccg tagcctcctg ttcgcgaaga acgcaacaga
      721 actcatgcgg aacgccgaaa acgagaggac cgtgaggggg gccggggtct cctccaggta
      781 aaggaggatc agggcgagca ccggcaccgc ggcgactgct gcggcgatgt agaccgcccg
      841 gtagggtacg agccccgggt agacggcgaa gaacgatatg atggccccgc cgacgagcgg
      901 ggcaagtgtc ctcccgacca gggttgcgga ggagtactgc ccgagcacct cccccttcct
      961 cccggcaaac cgcaccacga tcgtcgcggc gatcaccggc ccgaggattg ctgtcgccgt
     1021 cccgtggaag aaccgaatcg gtatcagcca gaacgggtcg gtgatgagga ggtagagcag
     1081 gggcgccgag aggaataccg ccccggatgc gatgagcaac cgccgcctgc ccaggtggtc
     1141 cgagaggacc cccaccggga acgagaacag gatgccggca agcggggaga ccgccgccac
     1201 gaggccgacg accgcgtccc cggcgcccag ggcctgcgag aagaggggca gcaccgggtt
     1261 cttcgctatc gtggtcgaga atatggcaaa aaaaccgatc gcaaagaggg tatacgcggc
     1321 atggccatcc ggcatctggt gacgctcgtt catggtcttt cccggccggc gttcaccggg
     1381 gacagttctc cggttgagag tgaccggtcc cggtatatcc catctccgaa atgttcccgg
     1441 aggagggggg cgctcccgcc atcccggggg atcgcgggag aggcgcctgc ccttcaaaaa
     1501 gccccagggt acgccgcgag cccgccccgg gcagatcccg gcccctcttc cgggctgacc
     1561 ggtaccccct ttccggaaag gaggctcctg caggcaccat gaaaaataat accattttta
     1621 taatattgat gaatatatct aacacgaggt ttctggtaca caaccagatc cgggaggcac
     1681 ccgaccgcgg ttcctcccgg tgtgttttgc ctctccggcg gtcgccagcg ccgggaggag
     1741 acgcggggat cgatggaccg ggagtctcac cacggcaccg cccgccggcg aatttcggcg
     1801 aagaccccgg ccccgggagc aggccatccg gagaggggac ctcccgcgag gctcaccgga
     1861 aaacgccgct actccggaat atcggccggt tgaatgcgga aagacagtat tctggggccc
     1921 ggcggcgctc cttcgccggt ctgtcatgta aaatcattac aaaacctata aaacggatat
     1981 gagaccaccc tgtacagttc attccggcct tccggcctga ctttccggga gacggacaat
     2041 ctgaacacga actgtagaat catactgatg ctcgatacga gactcaagat gtgaggagat
     2101 aaccatggcc aaaagaatgt tgagcgaatt cgaatcgtac gatctcctga aacaatacgg
     2161 cgtaccggta cctgaccacg tggtcgtcaa gactgccgcc gaggcgggca aggctgcgga
     2221 gaagataggt tttcccgtcg ttatgaagat ctactcgccc cagatcgtcc acaagagcga
     2281 tgcgggcggc gtgatcgtca gtatcccctc aaaatcggcc gccgaagagg cgtttgagaa
     2341 gatcgtcaag agtgcaaaag cctacaaccc cgaagccgag atcaagggcg tcatcgtcga
     2401 gcagcaggcg gcaccggggc ttgaactgat cataggcgga aagaccgatc cggcgttcgg
     2461 gaaggtgctc accttcggca tgggcggcac gctcgtcgag ctgatgaggg acgtcaccct
     2521 gcggatcctc cccatcacgg aagagacgat ccgggagatg atccaggaga tccacggcta
     2581 cccgatgatc aagggctatc gcgggacgaa accccgggac gaggaggcgc tcgtccagat
     2641 catgtgggcg gtcaactgct tctttgccga gaacacccac gtcgtggagt tcgacatcaa
     2701 ccctatccgg ctgtacgagt cgggggcctg catcgtcgac gcccgcatat tcgtcgacga
     2761 cgaagtcgtc gagaaggtgg caaaagagcg gccgttcgtg ccgatcgagt acttcacgcc
     2821 ccgctccatc gcagtcatcg gggcgtcgtc cgagcccaag aagatggggt acgccgtgat
     2881 gcacaacctc ctgcacttcc cgggccagct ctaccccgtg aacaacaagc ggccggaggt
     2941 gcaggggctc aaggcgtatg cctccatcct cgacatacca aacccggtcg acatggcggt
     3001 catcaccgtc cctgcaaagc acgtgccctc cgttatcgag gagtgcgggc agaagggcgt
     3061 atccatggcg gtcatcatca cggccgggtt caaagagacg ggcgaaggcg gcaaggcgct
     3121 tgaggaccgg atccttgcga tcgcgaaggg ttacggcacg cggatcgtgg gcccgaactg
     3181 tctcggtctg atcatccccc cgaagggcat cgacaccacc tacgtccacg aatccccgaa
     3241 acccggcaac atcgccttca tctcccagag cggcgccatc gtcaacaccg tcgtcgactg
     3301 gagcatcaag cacgatatcg ggttctccgc cgtcgtctcg gtcggcaacc aggcggacct
     3361 caacttcatc gacttcctcc ggttcgtgga gcgcgaccca aagaccaagg gcatcatcct
     3421 ctacatcgag gagatccagg acggcaagac gttcatgaag gtggcaggcg aggtcgccag
     3481 gaccaagccc atcgtggcga tcaagtccgg ttcgtccgag aaaggccagg cggcggcatc
     3541 gtcccacacg ggttcgctct cggggtcgta cgacgtctac atggaggcct tccgcaacgc
     3601 cggcgtcatc ccggtccaca ccctgaccgg cacgttccag gtcgcggaga tgctctcgct
     3661 cccgaagggc tacccccgcg gcaagcgtgc ggtcgtcgtc acgaacgccg gcgggtttgc
     3721 cgttctctcc tccgactacg ccgagcgcta cggcatcgac ctgatctccc tgccgccggc
     3781 ggtcctcaag gaactcaacg acctcctccc cgacttctgg aacaagaaca acccgatcga
     3841 cctcctcggc gacgcaaacg agaagcggtt tgaacagacg ttcaacgtgc ttgccagaca
     3901 ccaggactgc tgggacatcg cgttcgtcgt cgggttccca aacctcgtca tcgggtcgga
     3961 tcagctcgca aaccagatca taaagttctc ggagaagacc gagaacatga tcgtcgcgac
     4021 gctccttggc ggcgggtcca tggacagggg ccgaaagatc ctcaaagaga acggcatccc
     4081 gctcttcgag gaactcgact tcaccttccg ggtcatggga cggattctct ggcagagatt
     4141 ccggtaaatc tcctctccga atattttttg tcgcctctat tggtgctacc tccatagcag
     4201 gcgagatccg caacggtacc cggaacgggt cttcgcgcga ggtatacggg cggtgaaacg
     4261 gctttgcgcg gagccttgat gaggagttcc aggggcccat ccgccgaact tcgcagactt
     4321 ccgcgggagg ttgcccggtg cggataacca tacggcctcg cgagatgccg tagtgcccat
     4381 agcggcgacc gcctctgccg gcacccctca cgcatcacga tccccgggcg cctgcctttc
     4441 cttccttccg ccagaccacg atgaacgacg agaggacgag gagcgtgaag cctgcccaga
     4501 agagcggaac gatcgtgccg aagtccggcc cccgggtgag aagccgcacg atgccggcga
     4561 gcatcgatag cagtatggcc gcgatcacgg caaggagggg cgttatccgg acacgggccc
     4621 tctcccccga tacgaaggct gtctttggcg tcaccacgaa cggcagtttc ttccccgaga
     4681 ccgcatacca gatcgcccgg atgtagacga gagagagcgt gttgaagatc gcctgcgaga
     4741 acgcgaggtc gcgcggggag tagtgccgtt cgcgtatgct ggtgacgacc tggatggcga
     4801 tcaccgtgcc aaagaccacg gcaaagacca ccggaagcca tatcggcagg atggggatgc
     4861 caaagaggag cgtggcgacc gggaggagga agaacaccac gctcaaaccg ccgaagaggt
     4921 agaccgaggc gatcacggcg aactccagcc actggaccgg ggccaggctt ctcgggtgcg
     4981 ccacgaactg ccgcatcacc ttcccgagga gccgggtgtt cccgtaggcc cagcggagct
     5041 gctgcgtgaa gtacgccgcg aggtcggggg gcgccatgcc ttcggcatac acggcgtcga
     5101 tgtagacccc ccggtagcct ccggtgtgga agagaaaggc ggtggcgatg tcctcggtga
     5161 tggtggtctc gtccatcccc ccgatggcga gaaggtggcc gacgcgaaag atgcagtttg
     5221 agccggtcag catggtggtc cggttgatgg agagccccct gcagacatgt ttgttataga
     5281 tgtgctgctg gtaggagaac gtggcgctta tgaggttgtc cgggtccggc cggaagaact
     5341 ggggtgtctg gatgaacgag agggtggggt cggcctcgat gaccggtatg aggtcggcaa
     5401 ggattgccgg cttcactatc tggtcggcat cgagcaccag gaggtactgc gtatcgccgt
     5461 caaggaggga gagggcgtgg ttgatcgccc ccgccttgaa ccccttccgg tgatcgcggt
     5521 ggacgtaacg gatacgatag cggcggccga tctcctccat cgaaccgcgg aggcgctcgt
     5581 cggtcgagtc gtcgaggaga tagaccgtgc agtccggata ggcgatcgac gtgcacgccc
     5641 ggatgcagga ctcgaccacg cccggctcct cgttgtagac cggtatgaag accgcgacgc
     5701 ggccggcggg cggtgcggtc tcctgccgcg gcgcaagggg gtgttcgggg tagacgtaga
     5761 gcgaccgtag aaggtggtcg gcgtagttcg tgaactggat caccccgaac gccgtgacga
     5821 cgatgaggag gagagagagc gccctcgcag cggggctcca tccggctccg gcgagtatca
     5881 ggaggatatc gcgggagagg acgagcacga gcagcaccac gagcgtggcg gtgaggaacg
     5941 atatacgcgc tgacgtcttc atggggtatc caccactctc gcgtgcggga tgcctcgcgg
     6001 gcgcacggca ccgggctttc tgcaacggag ggtgcgcgag gcaatgtaag ttaccaccca
     6061 ctgtaccgga ttatttaacc cttatggtca cggccgcccc gggaccatga agactcatcc
     6121 ccggggagtt attcgccggc gcagggagcg gccgtgccgg tgccgcacaa cgttcgagag
     6181 tacgggcttt tattgatatc cgcgacacat ccggggcctt ccggagcaag gaagagaggg
     6241 gccgcacggc cgcacggcaa ccgacactct gaaataggtt gttccccatc catcaaatga
     6301 tacatatgat ggcaactccc tcgttaagga agtatgaact accgcccctg ccctacgcac
     6361 ctgacgctct cgagccgtac atatcaagag agcagctcac cctccaccat gacaagcacc
     6421 accaggcgta cgtgaccggg gcgaacgccg accttgagaa actggagaag gcgcggcagg
     6481 aagggaccga ggtcgacatg aaggcgctct taaaggagct ctcgttcaac gtgggcggcc
     6541 aggtcctgca caccctcttc tggccgaaca tggcgccggc aggctctggc ggcggcggca
     6601 aaccgggcgg cgcccttgca gaccggatag accgggagtg gggctccttt gaccggttca
     6661 aggcggagtt cacgaaggct gccgcgagca ccgaggggtc cggatgggcg gctctctcgc
     6721 actgcagcct gacggatcgc ctcatgatca tgcagatcga gaagcacaac aacaacgtct
     6781 acccgatgtt cggtatcctg atggtgctcg acgtctggga gcacgcctac tacgtcgact
     6841 acaagaacaa ccgcggccag ttcatagacg cgttctggaa cgtcgtgaac tgggatacgg
     6901 tgaacaagag actggaaaac ctctaacctc tcttttcggc ggaaaagcgc cccgtcagtc
     6961 ctctgttgaa acgccgtttc accgccccta cctgaactcg cgcgaagccg cgaaggccct
     7021 tccccggtcc ctgcaacccc caccgaggca acgcgagaga ccgtttcacc gctcatgccc
     7081 tctcaccttg cgcgtgaggc tgcagggatg cctgcacgca ggtttgaccc cacgcacagc
     7141 ctctcacctc gcgcgtgagg ctgcagggtt atcggcatca ggcgggccgc aaccgcaaat
     7201 ctcttatcgc ccgggacccg atccaattac ataaactcat ctatataaaa atctctgagg
     7261 ggggaacaaa ccatatgggc agagtaaatc cggtattcgt ccgaccggaa gagacgtttg
     7321 agatcctcga cgagcccggg gtgggtatcc tcgtgctcga ccgcgatctg cgggtggcct
     7381 gggcgaacgc ccgggccatg gatatcctcc cgatcggcaa cgacataccc ggcgccgatg
     7441 cccgccgggt cctcggcgcc ctcctgcagg aagaagagag cgtccggaaa ctcctcgacg
     7501 gcgcgagcga tatatccggc atcgatctcc cggcccgggg tccgcgggga gggaaacggc
     7561 ggatccgcta ctccagccgg aagatagagc aggggccgtt tgcggggata cgggtgctcc
     7621 ggctgcggga cgtcgccggc gaggagcggt accgctcgtt cttccgcgac ttccagggta
     7681 tcgcctaccg gacgacggag tccgggcccg tcctcttcca cggcgcggtg gaggcgatca
     7741 ccgggtatac ccaagacgat ttcctcgagg gagccgtatg ctgggatgcg gtcgtgcacc
     7801 ccgacgaccg ccccggtttt caggagtgcg ccggcgagct ccgggcggtc ccgggcctct
     7861 cgaccgagcg ggtctaccgg atcgtgcgaa aggacggcac gacccggtgg gtccgcgact
     7921 tcgcgaagaa cgtatgcaac gaggacggca cgcccgccct cgtccaggga accatccacg
     7981 acgccatcgg gcgaagagag gctgacgatg ggttgctctc gtcaaaccgg cagctctcgg
     8041 tcctgaacaa gatcatgggc gtcgccgcct cgtcgctctc gctggatgaa ctgctcgagg
     8101 cgtcgctctc ggtgacgatc gacctgctcg acctcgacgt ggggctgacc tacctgctca
     8161 acccggaacg gacactggcg ctcacgcgct accaccacgc cgccccgaag cgctacctcg
     8221 cccgcagccg gacgatcaag gtccaccact ggccctggaa cttcgtcttt gtcgccggac
     8281 aaccgcgcta catcgagcgg gacggcaacc cgggtgcggc cgaggaggag atcctcgcat
     8341 cgctcgaggt ctccgccctc gcctgcatac cgatcctcgc ggagtcggtc gtggtcgggg
     8401 cgctcttcct cgggaggcgg gaccggccgc ggatccgcga cgaggagcgc cgcctcctcg
     8461 aggcgatcgg gagagagatc ggctcgggcg tcctccggag catgctccac aaacggctgg
     8521 aggcggccca ccgcgagacg aacctctacc tggacgtcat gacccacgac ctcaagaacg
     8581 ccggcaacgt cgcaaacctc tactgcgacc tcctcatcga cgaaccgggg ggagccgccg
     8641 cagaatacgc aaagagactt cgggaaagta tccggaagag caccggcatc ctccggaacg
     8701 tctcgacgat ccgccggatt caccaggagc cgcccgacct caggccggtc ggccttgacc
     8761 gcgtcatctg cgcggagaga gagcgtatgc cggaggcggc cgtcacgctc gaggggacgc
     8821 cggcggaggt ctgggcggac gacctgctct cggagatatt tgcaaacctc atcggaaacg
     8881 ccgtcacgtt cggcgggccg ggcgccggca taacgatccg ggtggaaggg tgcgcaaaag
     8941 agaaccgcat ggtggtggtg accgtggagg acaccggacc cggcataccc gacggcgtga
     9001 aagaggcgct catccagcgg ctcggcgggg acgaagacca ggtccacggc gaagggctcg
     9061 gcctctccgt cgtcggcatg ctggtcgagc gctacggcgg ccgggtctgg atcgaggacc
     9121 gggtcagcgg caggccggac ctcggggcgg cgttccggtt cacgctccgc gaggttgccc
     9181 gcacggagcg taaaccgacg tgagggggag gatccccgct cacgccgctg ccttcgccgc
     9241 tccggcctgc cggacccgaa gccccgccgt gtagcggcgt gcccaggcgt agccgagcaa
     9301 tgcgccgatg gcgtttccgg cggcgacgcc ccaccagacg ccggagaggc cgaacccgag
     9361 gtctattgcg aacatccagg caagcagggt cgagaggacg acgttctgca ggaccgtgat
     9421 ggtcagggac cgtgccccaa gccccgtccc ctggaagaac gacgccgaga agaggccgaa
     9481 cccgaccatc gggtagacga ggctcatgac ccggagatac gacgtcagtt ccggcgtgat
     9541 ctccggtgtc gcgaagagcg cggcgatccc gggggccagt atgaacgtgg ccgcggcaag
     9601 cccgatgccg atgccggtcc cgagcctgat ggcgaagcgg agggcagact ccatccgggc
     9661 atacgcccgc gccccgtagg aggcgccggt gaccgcgacc accgacgttg ctatggcaag
     9721 tatcggggtt aagccgatgc tcacgacgcg ccacccggtc gagtagacgg cgacgccgtc
     9781 ggtgctcgat atgaggacga tgatgccgtt taagaccagg gtcatcaggg cgagcgagag
     9841 ctgttcggcg gcggccggaa ggccgacctg caggatatcg cgcgcgacgg cggggtcggg
     9901 ggcgaaccgc cggagtgaga gggatatgta ggtgtccctc ttcacgaaga gccagtagac
     9961 catcgggacg gcggcgacga tctcggcgat gatggtcgcg acggcggctc cctcgatccc
    10021 catgccgcac gtgtagatca ggatcgggtc gagtacgatg tttgccaccg cccctgcggc
    10081 catagcgtac atcgcccgct tcgcgtcgcc ctcaccgtgc atgagcgcgt aggcgacctc
    10141 gccgaagagg agggcaaacg tccccataaa gaggatccgg gcatacgcaa cggcaagccc
    10201 ggcggcgctg cccgcaccca tgagggcgaa tatatccccg gcaaagaggg cgagcgggat
    10261 ggtgacggca acggcaaggc cggtcatcag gaggatggtg tgcatggcaa cgctgcttgc
    10321 accgtcgcgg tcgcctgcac cgatcatgcg cgcaagagcc gcttcacccc cggtcccgag
    10381 gccgctcgct aacccgatgg tgatgatgaa gagcgggaat gagaacccga ccgcagcaag
    10441 agcatcggca ccgagacccg cgacccagaa cgcgtcgacg acgctgtaga gggtcatgag
    10501 ggtcatcgcg accatcaccg gcagagagag ccggaggatc gcggtcttcg gatcggcaag
    10561 gagtaacttt gttccttctg tttcgttctg gttgttatgt gagtgtgtgt cgtcttttgg
    10621 gacgcttgtc ggcatacata tcgcctgctg gcgtgcccgt gccgcgagct cccggttcaa
    10681 tcgggacgcc ctttgcgtgc ggtacggaca tcgcacctat ctgtgaacac tgatcatcga
    10741 acgctcctca gatattggac aggagtagaa acggcctgtt gttggccttc ccagttctgc
    10801 gatttccagg gcgcatagga cgatacaggt cagatgtggg cgcggattgt aattatactt
    10861 tcccacgggg agagggcaaa aaagtgcgag gagatcagcg ggccgccaat acctcgcgtc
    10921 tcttcacggc acggtcgtac atgagccagt tctcctccca ttcggggttt ctgtcgcgga
    10981 ggtgggagag caccctctgc cggatatcgg cggccggcat accgtcgtag gcgtcccgtt
    11041 cgacggcctc cccgatcgcc cgcgcttctt ctaacggcgc gcccgccttc agggcgctca
    11101 cgaccacttt ttcccgtgca aacggctctt tccggccgtc ggccttgacc acgttgacca
    11161 gttcgatcgc ctccgggcga ggaggggcgg tgaggggcta taacctttcc ggtcgccgcc
    11221 gggggggcgt caggccgccg tagacgatga cgtttcgcag ccctcggtcc ccgagcgccg
    11281 ccatcgcccg ttcgatcagg aagtcggagc cgaggaccca atcggccatc ctcttcgtct
    11341 ttttggcggt accgagttcc cggcccatcg ccgattgcca ggctcgctca taggcgcgga
    11401 ggggttcgtc gtgcagcagg tggcgggccg ccgcgatgcc ggcaagcctc ccgcagatca
    11461 tcgcggtcgc aatccccccg ccgttggagg cgacgacctg gccggcggcg tcgccgaccg
    11521 cgatgacgtt ctccctgacg gtctccggta tggggccggc aacggggacg aacccggccc
    11581 ggacgtcgcg gccgataaac cccatccgcg cgaggaaggc tctcaggagg agggtgacgg
    11641 acgtcgttct tccctgcacg ccgagcccga cgttggcgca gccctccttc gggaagaccc
    11701 acgcgtaccc gcccggggcg acgctgttcc cgaagtagag acggatgcgg tcgccgaact
    11761 ccccgtcgac ccggcaggtg acggcggggg cgaggacggc gcaccgctcc ataccggcgg
    11821 agcgggagac cagtgagagc gggccgtctg cggcgacaac gacgccggcc gtgattgtgc
    11881 cccggtcggt gatgattttc cgtccgtccc ggccgcgaaa tgtcgtctcc agcgagaggg
    11941 cggcgccggc acggacggcg gcatccgcga ggctgcggtc gagcgcatcc cggtcgaggg
    12001 tgtagccggg aaggtcgaac ccgtactccc ggccgccggg ggagacggcg acggcgcccc
    12061 ggattgcccg ccgggggaga tcgggttcgt cgggcacgga ggcggcgggg agcgcggttc
    12121 tgaggacctc ggacgaggcg ttgaactccc cgcagcagac gggcaggccg acctctcttc
    12181 gcttatcgat caggaggacg tcgagttctg cctccgccgc gtatcgtgcg gcggtgctgc
    12241 cggcggggcc cgcaccgacg acgaggacgt cgcaccgatc ctgcatcggc gctcacgtga
    12301 ggcgggggag gggataaaag agtggggtgc tctatccccg cgaccgcagc cgctctatcc
    12361 tctccgccac ccgcctccgc gcctcttttg tcgccgcggt gtcccggcgg accgtcacgt
    12421 cgagtatgtc gccctcccgg gcatccgcgg ggaggaggga gcgggggagc gtgaagcgga
    12481 tcgattcgtc ctcgcggatg aggaggacgg caagcccctc ctccacgcgg tcgaggcttg
    12541 cacggaaggt cgactcgtcc tgcatcctct catccctcca ggacgctgac gattgcgccg
    12601 ctcgcgtcag cgagcgtggc ggtatcgccg tcgttgttcc agaccgggga tgagcgtccc
    12661 cagtagaggt cggacgcggt ctcgttgccc gcgccggagt ggacggtgac gttcgagcct
    12721 gcgtcgagga cgaagaccgg gaaggtgtag gtgttgcgcg ctccttcgtc ggcgatcgtc
    12781 cagccggtga ggttgactgc ggtcgcgccg gcgttcgtga cggtgaccat ctcatccagg
    12841 aggctgaggt agg
//
LOCUS       DAOSEE010000058        12580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1936_length_12580_cov_221.940838, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000058 DAOSEE010000000
VERSION     DAOSEE010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 12580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12580
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1936_length_12580_cov_221.940838"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..1549
                     /locus_tag="PLI76_05910"
     CDS             50..1549
                     /locus_tag="PLI76_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2193 domain-containing protein"
                     /protein_id="HOI61602.1"
                     /translation="MKELYEKMINEAMAAQRADVDTVKAKRGRKFTVTDAKPYVEAVR
                     QMTAIGDQSRAVIDLHKNSVISHYEVIKGLTKTVRPEDDPFVEHYQTPVVLEILKDQD
                     EAFAKSVDAFVQAIAGAEALIGLEAVRHYGGFYGPTCVVDFALMPGSTSNVVNQVLAK
                     TDIPVEHKRAILAAKSWGMNTSYGIGDAFAKALEAGATPAEATAKEIATMQKIYRDPV
                     EAQAELMDEAGMTSFDARKYMSEYRRRMEASVRAAVDDGVHYGNILTVPAYSVGDVAH
                     HIAQSTFNMCKDDVVMAVIEAATDVMESSLRGSLDAYTSYWDVLTLATGASAAATEYI
                     LELDGFNAPMVVDLLTKRFHNYVLVNPARGAAVELHNCDFMDTIYRGWKILDKAQRLQ
                     NGSAGKLVPKVGRYPVDLAPIHANEVLMNPQWYAYPACAITVRFSALMRLADYPCLLT
                     SEPVTATMMTNIIALEKATAAAPMRGCKNCAAACLVDRRHQYCQWKEAV"
     gene            1566..1775
                     /locus_tag="PLI76_05915"
     CDS             1566..1775
                     /locus_tag="PLI76_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2180 family protein"
                     /protein_id="HOI61603.1"
                     /translation="MKCYYCALEGKESEAVAICIVCGMGLCMEHTIRKDVDVWEGGYP
                     LPSRRVKAPLPRILCPECYAALYGK"
     gene            1828..2574
                     /locus_tag="PLI76_05920"
     CDS             1828..2574
                     /locus_tag="PLI76_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HOI61604.1"
                     /translation="MTVSLGKRFVAEAVGTFILVFFGAGAAVVTLMLAVGTGQATPFN
                     IGIGALGGLGDWLAIGLAFGIAIAGAIYTFGRVSGCHINPAVTIALFATGRFPGRDVG
                     PYIAAQLLGAAAASLLFAWAIGPDAVTIGGLGATAPFHGIGYLQAIAIEAIGTFLLML
                     VIMGAAVDERATPGFAGLVVGLVVAGIITTTGNLTGASLNPARTFGPYLGDWLLAGQN
                     LWEFFPICIVGPIIGAVAAAILYDNLCCGD"
     gene            complement(2582..4213)
                     /locus_tag="PLI76_05925"
     CDS             complement(2582..4213)
                     /locus_tag="PLI76_05925"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOI61605.1"
                     /translation="MVKTTSKGPETTVADVLIAELAAWGVNLFFGIPGASSLPLVDAV
                     RKNQDVRYIVVRHEQTAAMAASAYNKFTGKVAACLTIAGPGATNLATGLYDAKEDHAS
                     VLSLNGQVKMQYSGPGGIQEIDQDAFFRPITVFNSTVADPMTAVKLLTRALRYAIIAR
                     GVAQLSVPNDIQAAPLKAAYCERETCLPSTRVAPTDEAVRAVAEAIDGAHRPVIVAGF
                     GALHAAGAVLDLATKIQAPIVTTLRGKGILPDDNEWVAGVHGELGTPHALMLVLKSDL
                     VIACGMSFSDHTGIPPGKRAVQIDIDPLQLGKHPLAVGVYGDCALALPRIAGQVRARE
                     DPDTRQWLAEKKREWLDRLDQEADPEATPIRPPYVMKVLSDLLPEDAIISIDVGDNQW
                     WFGRNFRMKRQRFAMSGYLGTMGFGLPGAIAAKLAYPDATVVCIVGDGGFSMVMGDLV
                     TTIKYDLPIVVVVLSNRELAMIREEQREEHYPPFGIDLTNPDFAAYAVACGGAGIRVT
                     RPQDLPGAVREALKMNTSVVIDVETDPERFPQKKK"
     gene            complement(4194..4436)
                     /locus_tag="PLI76_05930"
     CDS             complement(4194..4436)
                     /locus_tag="PLI76_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOI61606.1"
                     /translation="MELGYGPEFHLQDVNGMARWVCSVCDYEYNEEAGDPAAGIPPGT
                     RFEDLPADWRCPECMVGKDAFVRVDEEVEKNGENDL"
     gene            4639..5367
                     /gene="nfi"
                     /locus_tag="PLI76_05935"
     CDS             4639..5367
                     /gene="nfi"
                     /locus_tag="PLI76_05935"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866233.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HOI61607.1"
                     /translation="MEIRETHPFDLTPAEAAHLQEALRTRVRLTGDIGEVSLVAGVDA
                     SYVKGSNVIHAVVVALGYPDLAVVERVAARTETSFPYIPGLLTFREGPAVLKAFRKLR
                     SEPDVIFFDGQGIAHPRGLGIASHIGVLLDRPTVGVAKSLLVGSVEEPGVTRGSTAPI
                     IRDGETIGMAVRTKERTKPVYVSVGNRIDLFQAVDLVLATARRYRLPEPTRQAHLFAN
                     LARKTGIGEDRQTGLPSPEARREG"
     gene            5596..6786
                     /locus_tag="PLI76_05940"
     CDS             5596..6786
                     /locus_tag="PLI76_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOI61608.1"
                     /translation="MAAREIVPGVRSVGVIDWDRRIFDELIPLPDGTSYNSYLIRGSE
                     KTALIDTVDPVKTAELMANLDRAGAEKIDYIIANHAEQDHSGSIPAMLARFPEAKVVT
                     NQKCRDFLVDLLHIPVDRTIVVGDKETLPLGGKTLEFILAPWVHWPETMLTYLQEDRI
                     LFSCDFFGSHYATSSLYVPNEGVIYESAKRYYAEIMMPFRSSIKGHLTKLAARDIAMI
                     APSHGPIHKRPAFIMDAYRDWTDDAVKNMVVLPYVSMHGSTQAMVDHLVDALMQRGVP
                     VQPFNLPKTDIGDLAEALVDAATVVIGAPTVIFGPHPQAVYAAYLANLLRPKTRYATV
                     IGSYGWGGKTVDTVKNMLDRLKVELLEPVYIKGYPKEADFAALDRLADAIKKKHEEAG
                     IIVS"
     gene            7109..8128
                     /locus_tag="PLI76_05945"
     CDS             7109..8128
                     /locus_tag="PLI76_05945"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HOI61609.1"
                     /translation="MPPSDFKRVIAESPYVFVIGVAGDSGSGKTTFTRAIRAIFGRDL
                     VSMITIDDYHRYDREERKALGITPLVPEANRFDLLEEHLRDLKAGKTISKPVYNHDNG
                     KFDPPVPFSPTKILIVEGLHPFITRTLRDLVDFKLYVDPDPDVKRAWKIKRDVEQRGY
                     SPEAVIKEMEERKPDYEQYVAPQCRFADAVIRIAFSKYGRKVSEERNVYHVSLCQNGI
                     DRSIHNVDLSIDLYPILSLSQRSFMVEFTSEDVSGRPMGALTFDGELNYAVARKLEQN
                     IEIQTQVQPIDLVKNGAYLTAGGMAQLILAWRIINRRIAIEGAPGAAKEEEPAGYDGC
                     WCGRT"
     gene            8210..9667
                     /locus_tag="PLI76_05950"
     CDS             8210..9667
                     /locus_tag="PLI76_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOI61610.1"
                     /translation="MDRIEQFSTIAPDIGNIFRYISIGTAVPLIVAVIYREWDMILPM
                     LSVPITLFLLGTLLVRTPREEREAPLSAALMAVALIWLVMALASALPFSLGLGVSYLD
                     AVFEAMSGWTDTGLTVMRSVDDLPRTLLFWRSLMQWLGGIGIVAFTIAMASRTGLTQF
                     RLYRSEGRSEALMPSVVATGMEMWKIYLILTAASIGLILLSGVPLWDAVNIALTAIST
                     GGFSVHSAGIPYYHNPLLEVFIVPVMIAGALPFKLYYLLYRQRGIRVLDDQQARLLFM
                     LVALGIVVITWDLVTLSATGLTTAVHSALFMSAAAITSTGFQIVSPNEWASVTVLFLS
                     MLMVIGGASGSTAGGIKLSRVVLGAQSLVWWFRRMFVSGKVLVPFKYDGRVIPKNIAD
                     LEVSRNMLIIMLYFLTIFVATVLVMHLQPTAFDSSNVIFEVVSAMCNCGISAGFVSPD
                     MADSAKVLFILVMWIGRLEIIPVIMLVMGILKGFD"
     gene            complement(9824..10444)
                     /locus_tag="PLI76_05955"
     CDS             complement(9824..10444)
                     /locus_tag="PLI76_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61611.1"
                     /translation="MDPRFRSRLIAAIVLLIVVCSAFSVSPVAGFRLENRDGSGTDAT
                     LAEALVLQQSTRIREEFIKDITVYIDSRNRVFAQQGTYGNTSGLYVPAEDAIYIRSDR
                     HPAQADEAFARQVGYRVYHTMRFSESTVFPALDAGSGPCMARLSIPPGEEREAALFAE
                     AFMLYHASPALLKEDAPATYAYMDLLVKSGGDCAAVDGLYTHGRPA"
     gene            10675..11493
                     /gene="cfbC"
                     /locus_tag="PLI76_05960"
     CDS             10675..11493
                     /gene="cfbC"
                     /locus_tag="PLI76_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni-sirohydrochlorin a,c-diamide reductive
                     cyclase ATP-dependent reductase subunit"
                     /protein_id="HOI61612.1"
                     /translation="MKQIALYGKGGIGKSTTAANLSAALAGEGLDILQIGCDPKHDST
                     RMLMHGTWIPTVLDLIRERGDEGITVDDVVYRGFRGVRCVEAGGPEPGIGCAGRGIIA
                     TFQLLERLDALKGDVIVYDVLGDVVCGGFAMPMREGYAQEIYLVTSGELMSIYAANNI
                     AKAIARLSRRVRSRCSLGGVICNAKNIEGERDLVEDFARRINSRLIAYVPRDRVVQVA
                     ELHRQTVVEYAPDSSQAAVYEELGRAIYANRKTSIPTPLEMDELESFAFEFVQV"
     gene            11453..12514
                     /locus_tag="PLI76_05965"
     CDS             11453..12514
                     /locus_tag="PLI76_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HOI61613.1"
                     /translation="MNSSPSPSNSSRFEGCTLTGALSVLTEIRDAVCVIHGPSGCAHH
                     NFSLLHATLLSNDRLEVPRLLSTRLTEKDIIFGGEEALESSIARALSLSPAPSSVFVL
                     STCIVETIGDDTAAVSAKAWGVPVIAVPTAGFLGGVFETGVRNALSSAALLAESARER
                     TLSANLIGEKNLEYGVDENAAEIVRLLARLGIGINLRFVRGIATGDIGRLGSAAVNIL
                     REPALRPVGEDLKRRFSTPFVDSFPVGLAGTCRFLADIGRICSVDASAAIEEEQAFQR
                     EMLSRFADLEGSRVCFRSPHSMLEGDPAAEAVLAECAIALDLAVDPDGTPIPLPYPAP
                     VGTAGVRRMLNRWRVQIRG"
BASE COUNT         2364 a         4009 c         3936 g         2271 t
ORIGIN      
        1 acggcattcc tccccggcct gaaggccggg gtctcctgcc tatatgaaga tgaaagaact
       61 ctacgagaag atgatcaacg aggccatggc cgcccagagg gcggatgtgg atacagtaaa
      121 ggcgaaacgc ggccggaagt tcaccgtaac agacgcaaaa ccctatgttg aagcggtccg
      181 gcagatgacg gcgatcggcg accagagccg cgcagtcatc gatctccaca agaactcggt
      241 catatcgcac tacgaggtca tcaaaggcct cacgaagacc gtccgccccg aggacgatcc
      301 cttcgtcgag cactaccaga cgcccgtggt gctcgagatc ttaaaggacc aggacgaggc
      361 gtttgcaaag agcgtcgatg ccttcgtcca ggcgatcgcc ggcgctgagg ccctgatcgg
      421 ccttgaggcg gtccggcatt acggcgggtt ctacggtccc acctgcgtcg tcgactttgc
      481 gctcatgccg gggagcacga gcaacgtggt gaaccaggtc ctggcaaaga ccgacatacc
      541 ggtcgagcac aagcgggcga tactcgccgc aaagtcctgg ggcatgaaca cctcctacgg
      601 gatcggcgac gcctttgcca aagcgctcga ggccggggca accccggcag aggcgacggc
      661 aaaagagatc gcgacgatgc agaagatcta ccgcgatccg gtcgaagcgc aggcagaact
      721 gatggatgag gccggtatga cctcgtttga tgcccggaag tacatgagcg agtaccggcg
      781 gcggatggag gcatccgtca gggcggccgt cgacgacggg gttcactacg gcaacatcct
      841 gaccgttccc gcctacagcg tcggcgacgt cgcccaccac atcgcgcagt cgaccttcaa
      901 catgtgcaag gacgacgtcg tcatggcagt gatcgaggcg gccaccgacg tgatggagtc
      961 ttccctccgg ggctcgcttg acgcatacac gagctactgg gacgtcctca cgctcgcgac
     1021 cggcgcttcg gcggcggcga ccgagtacat actcgaactc gacgggttca acgcccccat
     1081 ggtcgtcgat ctcctcacga agcggttcca caactacgtc ctggtcaacc cggccagggg
     1141 agcggcagtg gagctccaca actgcgactt catggatacg atctaccgcg ggtggaagat
     1201 cctcgacaag gcgcagcggc tccagaacgg gtctgcagga aaactggtcc cgaaggtcgg
     1261 cagatacccg gtcgatcttg cacccatcca cgcaaacgag gtgctgatga acccgcaatg
     1321 gtatgcctac ccggcctgcg ccataaccgt ccggttctcc gccctgatgc ggcttgccga
     1381 ctacccgtgc ctcctgacga gcgaacccgt gaccgcgacg atgatgacga acatcatcgc
     1441 cctcgaaaaa gcgacagcgg ccgctccgat gcgggggtgc aagaactgcg cagcagcatg
     1501 cctggtcgac cgccggcacc agtactgtca gtggaaagag gcggtctaga gggagggaaa
     1561 ccggcatgaa gtgctactac tgcgcccttg aggggaagga gagcgaggcg gtggcgatct
     1621 gcatcgtctg cggcatgggg ctctgcatgg agcacaccat ccgaaaggac gtcgacgtct
     1681 gggaaggcgg ctatcctctc ccgagcaggc gcgtgaaagc gccgctcccg cggatactct
     1741 gccccgagtg ctacgctgcc ctgtacggga agtgaggcac tcccccggac tatttttgct
     1801 cgagtgacag gaaaatcagg tgaaaccatg actgtatctc ttggaaagcg gttcgttgcc
     1861 gaggccgtcg ggacgttcat cctggtcttc ttcggagcgg gtgccgcagt cgtcacgctg
     1921 atgctcgctg tggggaccgg ccaggcgaca ccgttcaaca tcggaatcgg ggcgctcgga
     1981 ggcctcggcg actggctcgc catagggctc gccttcggta tcgcgattgc cggagccatc
     2041 tacacattcg gaagggtatc gggctgtcac atcaacccgg cggtgacgat agccctcttt
     2101 gcgacggggc ggttcccggg ccgcgacgta ggtccctata tcgccgcgca gctcctcggt
     2161 gccgcggccg caagccttct cttcgcctgg gctatcggcc cggacgccgt cacgatcggg
     2221 ggcctcggtg caacggcacc cttccacggc atcggctacc tgcaggcgat cgccatcgag
     2281 gccatcggga cgttcctcct gatgctcgtc atcatgggcg ccgccgtcga cgaacgggcc
     2341 acaccgggtt ttgccggcct cgtggtcggt ctcgtggtcg ccggcatcat cacgacgacc
     2401 gggaacctga ccggcgcatc gctcaacccc gcgcgtacgt tcgggccgta cctcggagat
     2461 tggctgcttg ccgggcaaaa cctctgggag ttcttcccga tctgcatcgt cgggcccatc
     2521 ataggcgcgg tcgccgcagc gatcctttac gacaacctct gttgcggcga ttaagccgat
     2581 atcacttttt tttctgcggg aaccgttccg gatcggtctc gacgtcgatg acgacggacg
     2641 tgttcatctt caaggcctcc ctgaccgcgc cggggaggtc ctgcggccgg gtcacgcgaa
     2701 taccggcgcc gccgcaggcc acggcatagg cggcaaagtc ggggttcgtg agatcgatcc
     2761 cgaagggcgg gtagtgctcc tcccgctgct cctcgcggat catggcaagt tcgcggttgc
     2821 tcaggaccac cacgacgata gggagatcgt atttgatggt cgtgacgaga tcgcccatga
     2881 ccatcgagaa acctccatcc ccgacgatgc agacgacggt tgcatcaggg taggcgagtt
     2941 ttgccgcaat cgctccgggg agcccgaatc ccatcgtccc gaggtagccc gacatcgcga
     3001 accgctgtcg tttcatacgg aagttccgcc cgaaccacca ctggttgtca ccgacatcga
     3061 tggatatgat tgcatcctcc ggcaggaggt cggagaggac cttcatgacg tagggcgggc
     3121 ggatgggggt ggcctccggg tcggcctcct ggtcgagccg gtcgagccac tcccgtttct
     3181 tctccgcgag ccactgccgg gtatccgggt cctcccgcgc ccggacctgc ccggcgatcc
     3241 ggggaagagc aagggcacag tcgccgtaga cccctaccgc aagcggatgt ttcccgagct
     3301 ggagcggatc gatgtcgatc tggacggccc gcttacccgg cggtatgccg gtgtggtcgg
     3361 agaaactcat gccgcaggcg atcacgaggt ccgacttcag gacgagcatc agggcgtggg
     3421 gcgtcccgag ctcgccgtgc accccggcga cccactcgtt atcgtcgggg aggataccct
     3481 ttccgcggag ggtggtgacg atcggcgcct ggatctttgt tgcaaggtca aggaccgccc
     3541 cggccgcgtg cagggccccg aacccggcga cgatgaccgg gcggtgggca ccgtcgatcg
     3601 cttccgccac cgcccggacg gcctcgtcgg tcggcgcgac acgggtcgag ggaaggcagg
     3661 tctcgcgctc gcagtaggcg gccttgagcg gtgcggcctg gatatcgttc gggaccgaga
     3721 gctgggcgac gccgcgggcg atgatggcgt agcgcagtgc ccgggtgagg agtttgaccg
     3781 cggtcatcgg gtcggcgacg gtgctgttga agacggtgat gggccggaaa aacgcatcct
     3841 ggtcgatctc ctggatgccg cccgggccgg agtactgcat cttcacctgc ccgttgagcg
     3901 agagtacgct cgcgtgatcc tcttttgcgt cgtagagccc ggtcgcgagg ttcgtcgccc
     3961 ccggaccggc gatggtcagg caggcggcta ccttcccggt gaacttgttg taggcggatg
     4021 ccgccatcgc cgcggtctgc tcgtgccgga cgacgatgta gcggacgtcc tggttcttcc
     4081 ggaccgcatc cacgagcggg agagacgatg cgccgggaat gccgaagaag aggttgaccc
     4141 cccatgccgc gagttccgct atcaggacgt cggccacggt cgtttccggc cctttagagg
     4201 tcgttttcac cattcttctc cacctcctca tcgacccgca cgaacgcatc cttcccgacc
     4261 atgcactcag gacatcgcca gtcggcaggg aggtcttcaa accgcgttcc cggtggtatg
     4321 ccggcggcgg ggtcccccgc ctcttcgttg tactcataat cacagacact gcacacccat
     4381 cgcgccatgc cgttcacatc ctggaggtga aactccgggc catacccgag ttccatgtat
     4441 aaaaatgcct cgccccgggc gatcgggccg ggctcccgtg ccgccggcgc acgaggtttc
     4501 ccgccgccgc ctgcggttcc ccgtctgccc ttcaggggaa caactgcatg acgaagcgga
     4561 tccccatggt gggctgcatg ccccctgata tataccggca gggatacaac accacgtaaa
     4621 cccataccag gagtcaccat ggagatcaga gagacacacc cgttcgacct gacgccggcg
     4681 gaggccgccc acctccagga ggcgctccgg accagagtcc gcctcacggg cgatatcgga
     4741 gaggtctccc ttgtcgccgg cgtcgacgct tcctacgtga aaggctcaaa cgttatccac
     4801 gcggtcgtcg tcgccctggg ctaccccgac cttgccgtcg tcgagcgggt cgccgcccgc
     4861 acggagacgt cgtttcccta tatccccggc ctcctcacct tccgggaagg ccctgccgtc
     4921 ctcaaggcgt tccggaagct gcgatcagag cccgacgtca tcttctttga cggccaggga
     4981 atcgcccacc cgagggggct tgggatcgca agccacatag gcgtcctgct cgaccggccc
     5041 acggtcgggg ttgcaaagag cctccttgtc ggcagcgtgg aggagcccgg ggtcacccgg
     5101 gggtcgaccg cccccatcat ccgcgacggc gagacgatcg gtatggcggt gcggacgaaa
     5161 gagaggacga aaccggtcta cgtctccgtg ggcaaccgga tcgatctttt tcaggcggtc
     5221 gacctcgtcc tcgcgaccgc acgaagatac cggctcccgg aaccgacccg gcaggctcac
     5281 ctctttgcaa atcttgcgcg gaagacgggg atcggtgagg atagacagac cggcctcccc
     5341 tccccggagg cacggcggga agggtagcac atacctcacg catttccatg agagcgacgg
     5401 cgagtaccgg acgccgcgat ccggcggcgc aggtgccggt cgaaaccgtt atcaatcttt
     5461 gagaaggatt aacgtttacc ggtcggcgtg gcgcgacgat gccggtcgcc gaatggtgtg
     5521 gagcgtgaaa gatgtcggaa attactcaca cagactgtat aatgaccgga ataagcccga
     5581 tggaggcgga aagatatggc cgcgcgtgag attgtgccgg gcgtccggtc cgtcggggtc
     5641 atcgactggg accgcaggat cttcgatgaa ctgatcccgc tccccgacgg cacctcttac
     5701 aacagttacc tgatccgggg cagcgagaag acggcgctga tcgataccgt cgaccccgta
     5761 aagaccgcgg aactcatggc aaaccttgac cgggccggcg ctgaaaagat cgactacatc
     5821 atcgcaaacc acgccgagca ggatcactcc ggctcgattc cggcgatgct cgcccgcttc
     5881 cccgaagcga aagtggtgac gaaccagaag tgccgggact tcctggtcga cctcctccat
     5941 ataccggtag accggaccat cgtcgtcggc gataaagaga ccctccccct ggggggaaag
     6001 acgctcgagt tcatcctcgc accctgggtc cactggccgg agacgatgct cacctacctt
     6061 caggaggacc ggatcctctt ctcctgcgac ttcttcgggt cgcactatgc gacgagcagc
     6121 ctctacgtcc cgaacgaggg cgtgatctac gagtcggcaa aacgctatta cgccgagatc
     6181 atgatgccgt tccgctcaag catcaagggc cacctcacaa aacttgccgc gcgcgatatc
     6241 gccatgatag cgccgagcca cggcccgatc cacaagaggc cggcgttcat catggatgca
     6301 taccgtgact ggacggacga tgcggtgaag aacatggtgg tgctgccgta cgtctccatg
     6361 cacgggagca cccaggcgat ggtcgaccac ctcgtcgatg ccctgatgca gcgaggagtc
     6421 ccggtgcagc cgttcaacct cccgaagacc gatatcggcg atcttgcaga ggctctcgtg
     6481 gatgccgcga cggtcgtgat cggggcgccg acggtcatat tcggcccgca cccgcaggcg
     6541 gtctacgcag cataccttgc aaacctcctt cgcccgaaga cccggtacgc gacggtgatc
     6601 ggatcctacg gctggggcgg aaagacggtc gacaccgtga agaatatgct cgaccggctc
     6661 aaggtcgagc tcctcgaacc ggtctacatc aagggctacc cgaaagaggc ggacttcgcg
     6721 gcgctggacc ggctcgccga tgctatcaag aagaagcacg aagaggcagg tatcatcgtc
     6781 tcctgagaag aagcagactc cggagaatga gggggaagca tgcccgcggg ccggacggct
     6841 caccgggagg tcttcctccc cggcactccg ccccccgcgg tcgggaacat gcgacctctt
     6901 tctgcccgcc atgcgaagag gcaggcggga cgtgggtttg ccgggcacgc tctcctcccg
     6961 gggagccgtc ctgcggcaca ctgcccgggg acttgccatc gaaagcccgg ggcactcccc
     7021 gtaattggtt ttgggcgggc gagacggctc atccgccggc accgaaatct taacagccca
     7081 ggatacagac caatctgtga gctgacctat gcccccatca gatttcaagc gagtcatcgc
     7141 agagtcgccc tacgtcttcg tcatcggcgt agcgggagat agcggaagcg gaaagacaac
     7201 gttcacccgg gccatcaggg cgatcttcgg cagggatctc gtctcgatga taaccatcga
     7261 cgactaccac cggtacgacc gcgaggagcg caaagcactc ggtatcacgc cgctcgtccc
     7321 cgaggcgaac cggttcgacc tgcttgagga gcacctcagg gatctcaagg cgggaaagac
     7381 gatatcaaaa ccggtctaca accacgacaa cgggaagttc gacccgccgg tgcccttcag
     7441 ccccacgaag atcctgatcg tcgaagggtt gcacccgttc atcacccgga cgctccgtga
     7501 cctggttgac ttcaagctct acgtcgaccc ggaccccgac gtcaagcgcg cctggaagat
     7561 caaacgggac gtggagcagc gcgggtattc gcccgaggcc gtcataaagg agatggagga
     7621 gcgcaagccc gactacgagc agtacgtggc gccgcagtgc cggtttgcgg atgcggtcat
     7681 caggatagcc ttctcgaagt acggcaggaa ggtgagcgag gagcgcaacg tctaccacgt
     7741 ctccctctgc cagaacggta tcgaccggag catccacaac gtcgacctct cgatcgacct
     7801 ctacccgatc ctctccctct cgcagcggag cttcatggtc gagttcacct ccgaagacgt
     7861 ctcagggcgg ccgatgggag ccctcacgtt cgacggggag ttaaactacg ctgttgcgag
     7921 gaaactggag cagaacatag agatccagac gcaggtgcag cccatcgacc tcgtcaagaa
     7981 cggtgcatac ctgacggccg ggggcatggc ccagctcatc ctcgcctggc ggatcatcaa
     8041 ccggcgcatc gctatcgagg gtgctccggg cgcggcaaag gaggaagagc cggccggata
     8101 tgacgggtgc tggtgcggcc gcacgtgaga gaggagcgtt ctccggcgcc ccggggcaga
     8161 accgttatgg gagagaagat agataagagc gcccggagaa cgtgtaccga tggataggat
     8221 cgagcagttc tcgaccatcg cccccgacat cggcaatata ttcaggtata tcagcatcgg
     8281 caccgccgtg ccgctcatcg tcgcggtgat ctaccgcgag tgggatatga tcctccccat
     8341 gctctccgtc cccatcaccc tctttctcct cgggacgctt cttgtacgaa ccccacggga
     8401 ggagcgcgag gcgccgctct ccgccgccct catggccgtc gccctgatct ggctcgtcat
     8461 ggcgcttgca agcgcacttc ccttcagcct cgggcttggc gtctcgtacc tcgacgccgt
     8521 cttcgaggcg atgtcgggct ggaccgatac cggtctgacg gtgatgcggt cggtcgacga
     8581 cctcccgcgg acgctcctct tctggcggtc gcttatgcag tggctcggcg gcatcggtat
     8641 cgtcgcgttc acgatcgcga tggcgtcgag gaccggtctg acccagttcc gcctctaccg
     8701 gtcggagggg cgctcggagg cgctgatgcc gagcgtcgtc gcgacgggca tggagatgtg
     8761 gaagatctac ctgatcctga ccgcggcgtc gatcggcctc atcctcctct cgggggtacc
     8821 tctctgggat gcggtgaaca tagccctcac ggcgatatcc accggcgggt tctccgtcca
     8881 ctcggcaggc atcccctact accacaaccc gctccttgaa gtcttcatcg tcccggtcat
     8941 gatcgccggc gccctgccgt tcaagctcta ttacctcctc taccggcaga gggggatacg
     9001 ggtcctggat gaccagcagg cgcgcctcct cttcatgctt gtcgcgctcg gcatcgtcgt
     9061 gatcacgtgg gatcttgtca ccctcagcgc aaccggtctt accaccgccg tccactccgc
     9121 gctcttcatg tcggccgcgg cgatcaccag caccgggttt cagatcgtct ctccaaacga
     9181 gtgggcgagc gtgacggtcc tcttcctctc gatgctgatg gtgataggcg gggcgtcggg
     9241 gagcaccgcc ggcggcatca agctctcccg ggtggtcctc ggcgcccaga gcctggtctg
     9301 gtggttccgg cggatgttcg tctccgggaa ggtgctcgtt cccttcaagt acgacggtcg
     9361 ggtgatacca aagaacatcg ccgatctcga ggtctcgcgg aacatgctga tcatcatgct
     9421 ctacttcctc acgatcttcg ttgccacggt gctggtgatg cacctgcagc cgacggcctt
     9481 cgattcgagc aacgtgatat tcgaggttgt ctccgccatg tgcaactgcg gcatatcggc
     9541 ggggttcgtc tcgcccgata tggcggattc ggcaaaggtc ctcttcatac tggtcatgtg
     9601 gatagggcgc ctcgagatca tcccggtgat catgctcgtc atgggcatcc tgaaagggtt
     9661 cgattgaccg gctctcccgg ggcggacgct cccggcgccc cgcgaggggg gagcaccggg
     9721 tggcgcccga taacccggga tcgatctaac gatgatccag gcggcgtggg ataacacccc
     9781 gccgcgggat cgtttgaccg gggagaaccc catgatcggc cggtcacgcc ggccggccgt
     9841 gggtgtacag accatctacc gccgcgcaat caccgccgct ctttacgagc aggtccatgt
     9901 aggcatacgt cgccggggca tcctctttca ggagcgcggg agaggcgtga tagagcatga
     9961 aggcctcggc aaagagcgcc gcctccctct cctcgccggg cgggatggag agccgggcca
    10021 tgcagggccc ggacccggca tccagcgccg gaaagaccgt gctctcgcta aaccgcatgg
    10081 tgtggtagac ccggtagccg acctgccggg cgaaggcctc gtcggcctgc gccgggtgcc
    10141 tgtccgagcg gatgtagatg gcgtcctcgg cagggacgta gaggccgctc gtgttcccgt
    10201 atgtgccctg ctgtgcaaac actctgtttc tgctgtcgat gtagacagtg atgtccttga
    10261 tgaactcttc tctgatcctg gtgctctgct gcagcaccag agcctctgca agtgtcgcat
    10321 cggtgccgct cccatccctg ttctccagac ggaatccggc aaccgggctc acagaaaagg
    10381 ccgaacaaac gacgatcagg agtacaatcg ccgcaatcaa ccgtgatcga aatcgtgggt
    10441 ccatggttcg cattccctcc ggttaatcaa tggttctaaa ttcattgtta tatagttttc
    10501 taatatatta aaacagatta tatcaatcaa atacgtaaaa tatcgaatat tatctggatt
    10561 aataaggaaa taatccttaa ctaattaatt ctaatcttaa gaagtcttta tatataaaat
    10621 gcatggaagt ctcatccgac acctaattac ccttgcaagc gcataataag aacgatgaaa
    10681 cagatcgccc tctacggcaa gggtggaatc gggaaatcca ccactgcggc aaacctctcg
    10741 gcagcgctcg cgggggaggg gctcgacatc ctgcagatcg gctgcgaccc caagcacgac
    10801 agcacccgca tgctgatgca cgggacatgg atcccgaccg tgctcgacct gatccgcgag
    10861 cgcggggatg aggggatcac cgtcgacgat gtggtctacc gggggttccg gggggtgcgc
    10921 tgcgtcgagg cgggcgggcc cgagccgggg atcgggtgcg ccgggcgggg catcatcgcg
    10981 acgttccagc ttctcgaacg ccttgacgcc ctcaaaggcg acgtgatcgt ctacgatgtc
    11041 ctcggcgacg tcgtctgcgg cgggtttgcg atgccgatgc gcgagggata cgcgcaggag
    11101 atctacctgg tcacatccgg cgagctgatg tcgatctacg cggcaaacaa catcgcaaag
    11161 gccatcgccc gcctctcgcg ccgggtgcgc agcaggtgct cgctcggggg cgtgatctgc
    11221 aacgcaaaga acatcgaggg cgaacgggac ctggtggagg attttgcccg ccggatcaac
    11281 tcccggctca tcgcctacgt tccccgtgac cgggtggtgc aggtcgcgga gctgcaccgg
    11341 cagacggtcg tcgagtatgc gcccgattcc agccaggcgg cggtctacga ggaactcggg
    11401 cgcgcgatct atgcgaaccg gaagacgagc attcccaccc ccctcgagat ggatgaactc
    11461 gagtccttcg ccttcgaatt cgtccaggtt tgaggggtgc accctgaccg gagcgctctc
    11521 ggtcctcacg gagatacggg acgccgtctg cgtcatccac ggcccgtcgg gatgtgcgca
    11581 ccacaacttc tccctccttc acgccacgct gctctcaaac gaccgcctcg aagtcccccg
    11641 cctcctctcc acccgcctta cggagaagga tatcatattc ggcggggaag aggccctgga
    11701 gagcagcata gcgcgggcac tctcgctctc cccggccccc tcttccgtct tcgtcctctc
    11761 gacctgcatc gtcgagacga taggggacga caccgcggcg gtctctgcaa aggcatgggg
    11821 ggttccggtg atcgccgtgc cgaccgcagg gttcctcggc ggggtctttg agaccggcgt
    11881 cagaaacgct ctttcttccg ccgccctgct tgcagagtcc gcgagggaga gaaccctctc
    11941 ggcgaacctt atcggcgaga agaacctgga gtacggcgtc gacgagaacg ccgcggagat
    12001 cgtacgcctc cttgcccggc tcggtatcgg gatcaacctc cggttcgtgc gggggatcgc
    12061 gaccggagat atcgggcgcc tcggttctgc cgccgtcaat attctccgcg agccggcgct
    12121 ccggccggtc ggcgaggacc tcaagaggcg gtttagcacg cccttcgtgg actcgttccc
    12181 ggtcgggctt gccgggacgt gccggttcct tgcggatatc gggcgaatat gcagcgtgga
    12241 cgcctcggca gccatcgagg aggaacaggc cttccagcgc gagatgctct cgcggttcgc
    12301 ggatctcgag gggagccggg tctgtttccg gtccccccac tccatgctcg agggagaccc
    12361 ggccgcggag gcggtcctgg ccgagtgtgc catagccctc gaccttgccg tcgatccgga
    12421 tggaacccct atcccgctcc cgtatccggc gcccgtgggg accgccggtg tccgccggat
    12481 gctcaaccgg tggcgggtgc agatccgggg gtgagggggt gggcagtcga acacaggggg
    12541 ttgctgattc tcggccgttc ttccgggtaa gggttgatga
//
LOCUS       DAOSEE010000059        12497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1948_length_12497_cov_233.840781, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000059 DAOSEE010000000
VERSION     DAOSEE010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 12497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12497
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1948_length_12497_cov_233.840781"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="PLI76_05970"
     CDS             complement(<1..913)
                     /locus_tag="PLI76_05970"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOI61614.1"
                     /translation="MEQKKVVLAFSGGLDTSVCIPLLREHYGFDEIVTVAVDVGQPEE
                     EVIRATEKGRMLADNHYTIDAKEKYIAECIFPSIKANGSYEGYPMGTALARPLIAEEI
                     VKIAEKEGAFTVAHGCTGKGNDQLRFDFIFRSAGYKIVAPMREMNLTREWEICYAQEH
                     NIPVSVGKENPYSVDENCWSRSIEGGRLEDPAFHPPEEIYAWTVSPQAAPDAVEKIRI
                     EFEQGVPVALNGERLPGIAFIRELNRIAGRNGVGRNDMIEDRILGLKAREIYEHPAAT
                     VLLAAHRDLERLVLTRSELAFKHIVDDK"
     gene            1090..1305
                     /locus_tag="PLI76_05975"
     CDS             1090..1305
                     /locus_tag="PLI76_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61615.1"
                     /translation="MPAPFDAAGFPMPWQAKRDPVKSAIIRGKGDKITLNNEITDEVA
                     VYSQKHGIIPEKPEFVYISWIFKKAKL"
     gene            1329..2585
                     /locus_tag="PLI76_05980"
     CDS             1329..2585
                     /locus_tag="PLI76_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HOI61616.1"
                     /translation="MWRNSVIFEKAPINGTTPVPPIVSIRGLTKVFGPDTAKTLELHR
                     SGRSKREIYEETGATLALSDISLDILPGEIFVLMGLSGSGKSTILRCINRLIEPTNGE
                     IRIGGEDIVGMSRDELRETRRRRLGMIFQSFALLPHRTVLDNVAFGLEIQGIPPDERF
                     RKAADMLDMVGLGGYEASMPGELSGGMKQRVGLARALTSDPDILLMDEAFSALDPLIR
                     REMQDELLDLQQRLGKTIIFVTHDLDEALKLGDRIALMKDGAIVQVGTPEEILTNPKN
                     AYVEKFVADVDLTRVLSASDVMRRPEPVAQCTAGPRVALHLMEEHDIQGIFVVTRQHL
                     LRGLVTLEGAAGAIREGKQMADILITDLSVVAPDASLADIISLIVESPHPVAVADDTG
                     RLRGIISRGAILAALARKEEVNRATA"
     gene            2572..3414
                     /locus_tag="PLI76_05985"
     CDS             2572..3414
                     /locus_tag="PLI76_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline/glycine betaine ABC transporter
                     permease"
                     /protein_id="HOI61617.1"
                     /translation="MQPPKIPVGKAAEVLVDRIDTHLGWLLDGASDGLRFLVGGFEDI
                     LLLIPAPILILLAGILVWLVTRRDIWLAGLTVVGLLLIWNLGLWNLAMLTLALVVVST
                     VLALAIAIPLGIAAAGSETLNALLKPVLDFMQTMPSFVYLIPAVIFFGLGSVPGIIAT
                     VVFAMPPALRLTNLGIRQVPRELIEVADAFGSTPRQKLFKVQLPVALPTIMAGMNQCI
                     MLALSMTVIASMIGAGGLGYQVLVGIQRVDIGMGFEAGLAIVIIAIILDRITQNILPR
                     YGEQ"
     gene            3821..4696
                     /locus_tag="PLI76_05990"
     CDS             3821..4696
                     /locus_tag="PLI76_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HOI61618.1"
                     /translation="MVQKTKSILALTGIVLVLCLISAGCTGTGATEPKKQISIGYVTW
                     DCAIASTNVMKQVFEEAGYDVKPVAVDAGPLFQALSRGDVDFTTTGWLPHTHAHYWEQ
                     YGSRIDYVNENIPGAARIGLVVPAYVTIDSIEEMNGVADRFGGRIVGIEPGAGVMART
                     EQAIEEYGLDYALVASSSAGMAAELRTAISNEQWVAVTGWSPHWKFGRYDLKFLDDPK
                     GAYGGAEDIVTLARQGLAADDPEAYGILSRFEWTGEDIASVMTDIEGGMPEGEAAKKW
                     IDANRDRVDAWLGKA"
     gene            complement(4743..7913)
                     /gene="carB"
                     /locus_tag="PLI76_05995"
     CDS             complement(4743..7913)
                     /gene="carB"
                     /locus_tag="PLI76_05995"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOI61619.1"
                     /translation="MPRKSQIKKVLIIGSGPIQIGQAAEFDFSGSQACRALREEGVSV
                     VLVNSNPATIQTDPDTADVIYIEPLKADIVAKIIEKERPDGILSGMGGQTGLNMTVEL
                     AEMGALMGVEILGTPLEAIYRGEDREQFRDLMNAIGEPVPRSMILGSTKQIDEAIQYI
                     GLPAIIRPAYTLGGSGGGVAHTPDDMRRIIEIGLMRSRIHQVLVEESVAGWKEIEFEV
                     MRDAADTCIIVCGMENVDPVGVHTGESVVVAPILTLRDDEYQRLRNAAMKIIRALDVQ
                     GGCNIQFAYKDGDYRVIEVNPRVSRSSALASKATGYPIARVAAKIAIGLRLDEIVNPV
                     TGITPVSFEPAIDYVVAKVPRWPFDKFKSADRMLTTAMKSTGEVMAIGRTVEEAFKKA
                     LRSLDTDMRRHTNPNEIRMTLTAPTDERFGCLFDAFREGFTVREVADLTSIAPFFLEK
                     IKNIVDLERRLGTACNPGDIRAARRYGFSDEELQALTGKTADDIAALSGAPTYKMVDT
                     CAAEFPATTPYFYSTWEEECELTQNGAKKILILGSGPIRIGQGIEFDYCTVHAVMALR
                     EEEGVEVHIINNNPETVSTDADTSDRLFFEPMQLEDVMNILKKDDYYGVMVQFGGQNS
                     VNLAVPLEAEIRRLGLPTKILGTSPDAMDVAEDRDRFSRLLARLGIPSPANSSAHSEE
                     EARTMARAIGYPVLVRPSYVLGGRAMELVHDEVELESYITEAVRVSRKHPVLIDSFLR
                     SAVEIDVDAVSDGTDVLIGGIMEHIEWAGVHSGDSACVIPPQSLSPSVIARVRDYTKR
                     LALGLGVVGLINIQYAVQDDVVYVLEANPRASRTVPFVAKATGIALAKIAAKVMVGRK
                     LADMGIVEPAIKYVAVKEVLLPFNKLPGVDTVLGPEMKSTGEVMGIDYDFGRAYYKAS
                     TAAENTLPTAGNIFISVTDEQKEEILPIARKLRELGLSLYGTSGTVDFLTQNGVEANL
                     VRKVQEGSPNVIDIMRSGGIRLIINTPADKASRQDHIQIMRAAVDYGIPYITTLQAAR
                     AAAMAIDAIQREEITLEPLGHYHGLA"
     gene            complement(7913..8941)
                     /gene="carA"
                     /locus_tag="PLI76_06000"
     CDS             complement(7913..8941)
                     /gene="carA"
                     /locus_tag="PLI76_06000"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOI61620.1"
                     /translation="MKAVLGLEDGTFVAGNGFGVEGICSGELVFTTQMTGYMEALTDP
                     GYAGQLLMFTYPLIGNYGVDRENFESPRVHARGCVVREIACVPAMQPSVAEFFRENGL
                     LGISGVDTRRLTVKTRTVGTLRASLIVGSDDGEEAVRCARVSPPAGDADLIGSVSCPA
                     PYRIAGAGKRVAVIDLGVKRSILTGLSRSGADIRVFPHTATPGDIMAAEPDALLITNG
                     PGDPGKAADAIRCAREVVGTMPVIGIGMGTQVAALALGAGTYRMKFGHRGANQPVRYR
                     DGSISITTQNHGFAVDGETLPEGCTVSYRNVNDGTVEGFEDKDLAITCVQFHPDIQFI
                     DRVYRGIP"
     gene            9086..9967
                     /locus_tag="PLI76_06005"
     CDS             9086..9967
                     /locus_tag="PLI76_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOI61621.1"
                     /translation="MSGGTIEKIRAVETPEGTFTIRLIHDDLESIYPGMVRYTVDILC
                     GKEPVATYAVNSYEVPPGSLVDTREAAEVLFSRVAHDVASRPHTYVRPRHLTRPLPGS
                     GAYDVVILQGSPRQFGNSARIASWCSDEAARAGFSSRVFFLHELDIRPCIGCYACYNF
                     GYCPIDDDMPGIVRALESASVIVVCTPVYTGTVPAGLKAVIDRCQWLHAREKVLGREV
                     HARGLLVAVAGQRGSEPFVCITRVVGMFMGNLGIRPAAPVLIDDLDRARDVGKVEGAE
                     DDVRAALRALLAPRDEG"
     gene            9995..10339
                     /locus_tag="PLI76_06010"
     CDS             9995..10339
                     /locus_tag="PLI76_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61622.1"
                     /translation="MAEVTVEIVGFMESECGPFPCDETRSCGLETCYPSNSLIDAIGA
                     LRDELLAQYADRVEVKMTLLDEGMPDHIRKIIEERHPPIPIVLVNGRLTSIGRVSLDL
                     IKEEIEYALEET"
     gene            complement(10336..10917)
                     /locus_tag="PLI76_06015"
     CDS             complement(10336..10917)
                     /locus_tag="PLI76_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HOI61623.1"
                     /translation="MIDKFLEGNKRFLERDFVKAPEHYGSLVSGQHPTVLWIGCSDSR
                     VDPERITGSRAGELFVQRNIGNIVPVHDWNFATVLEYAINHLEVGDIVICGHSDCGAI
                     KGLDHEGKDAYIPLWLNNAREAKRRVDDRIQMPKTPEEEKTRLRLIEIENVGLQLEHL
                     RTYPPVMAAEKGGRVRLHGLYFDLETGELKKMF"
     gene            complement(11335..>12497)
                     /locus_tag="PLI76_06020"
     CDS             complement(11335..>12497)
                     /locus_tag="PLI76_06020"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOI61624.1"
                     /translation="EGVGRVAAGTMSEEELGELECAAMPGCGSCQGLYTANTMACVTE
                     ALGLSLPGCAAIPAVDAAKLRIARESGERVVGLAREGIRPRDIVSPASLANAIRVDMA
                     LGGSSNTVLHLMAIAREAGVPLDLETFNTIGEETPHICHMQPGGPHSMLTLYRAGGIP
                     AVLAMLAGHLDDAPTVSGRSILDIAKTARVADPDIIRTVKDPVNPAGGLKVVRGTLAP
                     DGAVVKCAAVPAAMWRHRGPARVFDGETAAMEAILHRDIVEGDVVVIRYEGPRGGPGM
                     PEMLAPTSALMGLGYARVALVTDGRFSGGTRGPCIGHVAPEAAVGGPIALLEDGDAVA
                     IDLYAKSLDLLVDPGTLEERRRAWKPPERRLTGVLARYARTVEQANLGAVQR"
BASE COUNT         2262 a         3918 c         3936 g         2381 t
ORIGIN      
        1 acttatcgtc gacgatgtgc ttgaacgcaa gctccgagcg ggtcaggacg aggcgttcga
       61 ggtcgcggtg cgcggcaagg aggacggtcg cagccgggtg ctcgtagatc tcgcgggcct
      121 tcaggccgag gatccggtcc tcgatcatat cgttgcggcc cacgccgttc ctgccggcta
      181 tccggttcag ttcccggata aaggcgatgc cggggagcct ctctccgttg agggcgaccg
      241 ggacgccctg ctcgaactct atccggatct tctccacggc gtcgggggcc gcctgcggcg
      301 ataccgtcca ggcgtagatc tcctcgggcg ggtggaaggc cgggtcctcg agcctgccgc
      361 cctcgatgct ccggctccag cagttttcgt cgacgctgta ggggttctcc ttcccgacgc
      421 tcaccggtat gttgtgctcc tgtgcgtagc agatctccca ctcgcgggtc aggttcatct
      481 cccgcatcgg tgcgacgatc ttatagcctg cggacctgaa tatgaagtcg aaccggagct
      541 ggtcgtttcc ttttccggtg cacccgtgcg caaccgtaaa ggcgccttcc ttctctgcga
      601 tcttcacgat ctcctcggcg atcagcgggc gggcgagtgc cgtgcccatc gggtaaccct
      661 cgtatgaccc gttcgccttt atcgagggga agatgcactc cgcgatgtac ttctccttcg
      721 catcgatggt gtagtggtta tctgcaagca tccggccctt ctctgttgcc cggataacct
      781 cctcctcggg ctggcccacg tcgacggcaa cggtgacgat ctcgtcaaac ccgtagtgct
      841 cgcgcaggag ggggatacag acggaggtat caagaccccc cgaaaatgcc aggacgacct
      901 ttttctgttc catggtaata atctccgcgt tcctgatacc tgttctgctg acagtctgga
      961 ataatctaca ttgatatgac ctttcacccg ataaaagaag cggtatggat gagcgcaccg
     1021 gcaggaatca gacttcaatc gtgtaattcc cccggtccgg gcgtgcaggg gcattgatga
     1081 gggaggcggg tgccggcgcc cttcgacgca gccggttttc cgatgccgtg gcaggcgaag
     1141 agagatcccg tcaaatcggc cataataagg ggcaaaggcg acaaaataac cctgaataac
     1201 gagattacag atgaagtggc agtctattcc caaaagcacg gcataatccc ggaaaaacca
     1261 gaattcgtgt atatatcgtg gattttcaaa aaggcgaaat tataaaagag attagatcga
     1321 aaccctgaat gtggaggaat tccgtcattt ttgagaaggc acccataaac ggcacaactc
     1381 ccgtcccgcc gatcgtcagc atcaggggcc tgacaaaggt gttcggcccg gacactgcaa
     1441 aaacgctcga actccataga tccggccgct ccaaacggga gatatacgaa gagaccggcg
     1501 cgacactggc cctcagcgac atatccctcg acatcctgcc cggtgagata ttcgtcctta
     1561 tgggactctc cgggagcgga aaatcgacga tcctgcgctg catcaaccgg ctcatcgaac
     1621 ccacaaacgg cgagatacgg ataggcggcg aggatattgt cgggatgagc cgggatgagc
     1681 tgcgggagac ccggcggcgc aggctgggca tgatcttcca gagctttgca ctcctcccgc
     1741 accggacggt gctcgacaac gttgccttcg ggcttgagat ccagggcatc ccgccagatg
     1801 agcgtttccg gaaagcggca gatatgctcg atatggtggg gctcggcggc tacgaggcaa
     1861 gcatgccggg cgagctctcc gggggcatga agcagcgggt cgggcttgcc cgggcactca
     1921 cgagcgaccc ggacatactc cttatggacg aggcattcag cgctcttgac ccgctcatcc
     1981 gcagggagat gcaggacgaa ctgctggacc tccagcaacg gctcggtaag accatcatct
     2041 tcgtgactca cgatctggat gaggccctga aactcggcga tcgtatcgcc ctgatgaaag
     2101 acggcgcgat cgtgcaggtc gggacgccgg aggagatcct gacgaacccc aagaacgcct
     2161 acgtcgagaa gttcgtcgcc gatgtggacc tgaccagggt gctctcggca agcgacgtga
     2221 tgcgccgtcc cgaaccggtt gcccaatgca ccgcagggcc gagggtggcc ctgcacctca
     2281 tggaggagca cgacatccag gggatattcg tggtcacccg ccagcacctg ctccgcggat
     2341 tggtgacgct cgaaggcgct gccggggcca tccgggaggg aaaacagatg gccgatatcc
     2401 tcataaccga tctctccgtc gtggctcccg acgcatcgct ggccgacatc atatcgctga
     2461 tcgtcgagag tccccacccc gtggcggtgg cggacgatac cggcaggctc aggggcatca
     2521 tatcgcgcgg cgccatactc gccgccctgg cgcggaaaga agaggtgaac cgtgcaaccg
     2581 cctaagatcc ccgtggggaa ggctgccgag gtgctcgtcg accggatcga cacccacctg
     2641 ggctggctcc tcgacggcgc aagtgatggg cttcggttcc tcgtgggcgg gtttgaggat
     2701 atccttctcc tcataccggc acccatcctg atccttcttg caggcatcct ggtctggctc
     2761 gtcacccggc gcgacatatg gcttgcaggg ctgaccgtcg tcggtctgct cctgatctgg
     2821 aacctcggcc tctggaatct cgccatgctc accctggcgc tcgtcgtcgt ctcgacggtt
     2881 ctcgcgctcg cgatagcaat cccgctcggg atcgcggctg cagggagcga gaccctgaat
     2941 gcgctcctca aaccggtcct cgacttcatg cagaccatgc cctcgttcgt ctacctcatc
     3001 cccgccgtta tcttctttgg cctcgggagt gttcccggca tcatcgcgac ggtggtcttt
     3061 gcaatgcctc cggcactgcg cctcacgaac ctcggcatcc ggcaggtgcc gcgggagttg
     3121 atcgaggtcg cggatgcctt cggttccacg ccccgccaga agctcttcaa agtgcagctc
     3181 cctgtcgcgc tccccaccat catggccggg atgaaccagt gcatcatgct cgccctctcg
     3241 atgaccgtca tcgcgtcgat gatcggggcc ggagggcttg gctaccaggt gctggtgggc
     3301 atccagcggg tggatatcgg catggggttt gaggcggggc ttgccatcgt catcatcgcg
     3361 atcatcctcg accggatcac ccagaacatt ctgccgcgat acggggaaca gtgacccttg
     3421 ccgatcccca cccggggcac gtcaacccgg ctgtccgccc cggatccgcc gcagttctgc
     3481 cgggacgccc ggcgacggca gatcggcgcc cttgccgggg gaccggggac ggtctccggc
     3541 ggcatgacgc tcaaaattcg atttattgag gctccgtccg ccaaacggaa cccgtaaaac
     3601 ctaaataatg tgagtatcat acgctcaagc ggtcaatgtc tggccttttt gcctggggtg
     3661 caaacacacc aggagtggcg catatcgcca tctctgcccg atgtccgatc cagcggcctt
     3721 ctgtcgtttc ggacatgtcg tgagagcccg gaaccccata ttgccgggcc ctgaacgtta
     3781 tcaagccgcc ggacggcggc aaaaacctac gaggtgatcc atggttcaaa agaccaaaag
     3841 tatactcgca ctgacaggta tcgttctggt gctctgtctt atatccgccg gttgcacggg
     3901 tacaggggca accgaaccca aaaaacagat cagcataggc tacgtcacct gggactgtgc
     3961 catcgcaagc acaaacgtca tgaaacaggt ctttgaagaa gccggctacg acgtcaaacc
     4021 ggtcgcggtc gacgcagggc cgctcttcca ggcgcttagc aggggagacg tcgactttac
     4081 gacgaccgga tggctcccgc acacccatgc gcactactgg gagcagtacg gcagccggat
     4141 cgattacgtc aacgagaaca tccccggagc ggcacgtatc gggctcgtcg tcccggccta
     4201 cgtgaccatc gactctatcg aggagatgaa cggcgttgca gaccggttcg gcggcaggat
     4261 cgtcggcata gaacccggag ccggggtcat ggcccggacc gagcaggcca tcgaggagta
     4321 cggcctcgac tacgcgctgg ttgcaagcag cagtgccggc atggccgctg agttgcgcac
     4381 cgcgatcagc aacgagcagt gggtcgcggt gacaggctgg tcgccgcact ggaagttcgg
     4441 ccgctacgac ctcaagttcc tcgacgaccc gaagggagcc tacggcgggg ccgaagatat
     4501 cgtgaccctc gcgcggcagg gtcttgctgc cgacgatccg gaagcctacg gcatcctgag
     4561 ccggttcgag tggacgggcg aggatattgc ctcggtgatg accgatatcg agggcggcat
     4621 gcccgaagga gaggccgcga agaagtggat cgatgccaac cgcgacaggg tcgatgcctg
     4681 gcttggaaaa gcataaccct ctctcttttc accccggtcc ggaggagggt gataccccct
     4741 gatcacgcca gcccgtggta gtgcccgagc ggctcgaggg tgatctcctc gcgctggatg
     4801 gcatcgatcg ccatcgcagc ggcccgcgcg gcctggagcg tcgtgatgta ggggatgccg
     4861 taatcgacgg cggcccgcat gatctggata tggtcctgcc gggaggcctt atccgccggg
     4921 gtgttgatga tcagccgtat tccaccggaa cgcatgatgt cgatgacgtt tggagaaccc
     4981 tcctggacct tccgcacgag gtttgcctcg acgccgttct gggtgaggaa gtcgaccgtc
     5041 ccgctcgtgc cgtagaggga gaggccgagt tcccggagct tcctcgcgat cggcaggatc
     5101 tcttctttct gctcgtccgt caccgatatg aatatgtttc ctgccgtcgg cagggtgttc
     5161 tccgcggcgg tgctcgcctt gtagtaggcg cggccgaagt cgtaatcgat ccccatcacc
     5221 tcgccggtgc tcttcatctc gggaccgagc acggtgtcga cgccggggag tttgttgaag
     5281 gggaggagaa cctccttcac cgcgacgtac ttgatcgcgg gctcgacgat gcccatatcg
     5341 gcaagctttc tccccaccat caccttcgcc gctatctttg caagcgcaat ccccgtcgcc
     5401 ttcgcgacga acggcaccgt ccggctcgcg cgcgggtttg cctcgagcac gtagacgacg
     5461 tcgtcctgga cggcatactg gatgttgatc aggccgacga cgccaaggcc gagggcgagc
     5521 ctcttcgtgt agtcgcgcac ccgcgcgatc accgagggcg agagggactg cggcgggatg
     5581 acgcaggccg agtcgccgga gtggacgccg gcccactcga tatgctccat gatgccgccg
     5641 atcaggacat ccgtcccgtc agagaccgca tcgacgtcga tctcgacggc gctccggagg
     5701 aaggagtcga tcaggaccgg gtgcttccgg ctcacgcgga cggcctccgt gatgtaactc
     5761 tcaagctcga cctcgtcgtg gacgagctcc atcgcccgcc cgccgaggac gtaggacggc
     5821 cggaccagga ccgggtagcc gatcgcccgg gccatcgttc gcgcctcctc ctccgagtga
     5881 gcggagctgt ttgccgggct cggtatgccg agccgggcaa ggagccggct gaaccggtcg
     5941 cggtcttccg cgacatccat ggcgtcgggc gacgtgccga ggatcttcgt cggaaggccg
     6001 aggcgccgga tctcggcctc gagcggcacg gccaggttca ccgagttctg gccgccgaac
     6061 tggaccatga cgccgtagta gtcgtccttc ttgaggatgt tcatgacatc ttcaagttgc
     6121 atcggctcaa agaagagccg gtcggacgtg tcggcgtcgg tcgagacggt ctcggggtta
     6181 ttgttgatga tatggacctc tactccttcc tcctcgcgga gcgccatcac cgcgtggacg
     6241 gtgcagtagt cgaactcgat cccctggccg atccggatcg gccccgaacc gagtatcagg
     6301 atcttcttgg caccgttttg ggtgagttcg cactcctcct cccaggtcga gtagaagtag
     6361 ggcgtggtgg ccgggaactc agcggcgcag gtatccacca tcttgtaggt cggcgccccg
     6421 gaaagagccg cgatatcgtc ggccgtcttc ccggtaagag cctgcaattc ttcgtccgag
     6481 aacccgtagc gccgggcagc ccgtatatcc ccagggttac aggcggttcc gagcctccgc
     6541 tcgaggtcca cgatgttctt gatcttctca aggaagaacg gcgcgatgga ggtgaggtcg
     6601 gcgacctccc tgaccgtgaa tccctcccgg aaggcgtcga agaggcaccc gaaccgctcg
     6661 tcggtcgggg ccgtgagggt catccggatc tcgtttgggt tcgtgtgccg ccgcatatcg
     6721 gtatcgagcg accggagcgc cttcttgaac gcctcctcga ccgtccggcc gatagccatc
     6781 acctcgccgg tgctcttcat cgccgtggtg agcatccggt ccgcggactt gaacttgtca
     6841 aacggccacc ggggcacttt cgcgacgacg tagtcgatgg cgggctcaaa cgatacgggg
     6901 gtgatgccgg tcacgggatt cacgatctcg tcgaggcgga gccctatggc gatctttgcc
     6961 gccacccggg cgatcgggta gccggtcgcc ttggatgcga gcgcggagga gcgggagacc
     7021 cgggggttca cctcgatgac ccggtagtcg ccgtctttgt aggcgaactg gatgttgcac
     7081 ccgccctgga cgtcgagggc ccggatgatc ttcatcgcag cattccggag cctctggtac
     7141 tcgtcgtccc gcagggtgag gatgggcgca acgacgacgc tctcgccggt atggacgcct
     7201 accgggtcga cgttctccat gccgcagacg atgatgcagg tatcggctgc atcccgcatc
     7261 acctcgaact cgatctcctt ccacccggcg acgctctctt ccaccagcac ctggtggatc
     7321 cgggagcgca taagcccgat ctcgatgatc cgacgcatat cgtccggcgt atgcgcaaca
     7381 ccgccgcctg agccgccgag ggtgtaggca ggcctgatga tcgccgggag accgatatac
     7441 tggatggcct cgtcgatctg ctttgtgctc ccgagtatca tgctccgggg gacgggctcc
     7501 ccgatagcgt tcatcaggtc gcggaactgc tcgcggtctt ctccccggta gatcgcctca
     7561 agcggcgtcc cgaggatctc cacgcccata agtgcgccca tctccgcgag ttccaccgtc
     7621 atgttgaggc cggtctggcc gcccatgccg ctcaggatac cgtccggtct ctctttttcg
     7681 atgatctttg ctacgatatc ggccttcagg ggctcgatat agatgacgtc cgccgtatcg
     7741 gggtcggtct ggatcgtcgc gggattagag ttcacgagga cgacactgac cccctcctcc
     7801 cggagcgccc ggcaggcctg cgacccggag aagtcgaact cggccgcctg cccaatctgg
     7861 atgggccctg aaccgatgat gaggactttc ttgatctggg atttgcgcgg cattacggta
     7921 tccccctgta tacgcggtca atgaactgta tgtccgggtg gaactggacg caggtgatgg
     7981 cgaggtcttt atcctcgaac ccttcgaccg tgccgtcgtt cacgttccgg tacgagacgg
     8041 tgcatccctc gggcagggtc tctccgtcca ctgcaaaacc gtggttctgg gtggtgatgg
     8101 atatgctccc gtcccggtaa cggaccggct ggtttgcccc ccggtgaccg aacttcatcc
     8161 ggtaagtccc ggcaccgagc gcgagagcgg ccacctgggt gcccatgccg atgccgatga
     8221 ccggcatcgt gccgacgacc tcccgggcgc accggatggc gtcggccgcc ttccccgggt
     8281 cgccggggcc gttcgttatg aggagcgcat cgggctccgc cgccatgata tcgccgggcg
     8341 tggcggtgtg cgggaagacg cggatatcgg cgccggagcg gctgaggccg gtaaggatac
     8401 tccgtttcac accgaggtcg atgacggcga cccgtttccc ggcgccggcg atacggtacg
     8461 gcgcagggca ggagacgctc ccgatgaggt cggcatcacc tgccggggga gagacccggg
     8521 cgcaccggac agcctcctct ccgtcatcgc tgccgacgat gagcgatgcg cggagcgtgc
     8581 cgaccgtcct ggtctttacc gtcagtctgc gggtatcgac gcccgatata ccgaggaggc
     8641 cgttctccct gaaaaactct gcaaccgatg gctgcatggc gggcacgcag gcgatctcgc
     8701 gcacaacgca gccccgtgca tgaacacgcg ggctctcgaa gttctcccgg tccacaccat
     8761 agttcccgat gagaggatac gtgaacatca gtagctgccc ggcatacccg gggtccgtca
     8821 acgcctccat gtaaccggtc atctgcgtgg taaagacgag ttcaccggaa catatccctt
     8881 caacgccaaa gccgttccct gcaacaaatg ttccatcttc aagacccagg accgccttca
     8941 tgaattacca tatcatgtgg aacggattcc ttaataaccg tgatgacgga tcggatgtgt
     9001 tctccggaaa tcccgcagga aacccggaaa tgggggtttt tatcgcccgg aataccgggg
     9061 ccgggaccat agggggcggc gcgcgatgag cgggggtaca atagagaaga ttcgcgcggt
     9121 cgagacgccg gaggggacgt tcaccatccg gctcatccat gacgacctcg agagcatcta
     9181 tccgggcatg gttcggtata ccgtcgatat cctctgcggc aaagaaccgg tcgccaccta
     9241 cgccgtcaac tcctacgagg tccctcccgg gtctctcgtc gatacccggg aggcggcgga
     9301 ggtcctcttc tcacgggtcg cgcacgatgt ggcgtcccgt cctcatacct acgtccgccc
     9361 ccgccacctc acgcgcccgc tccccggcag cggcgcctat gacgtcgtca tactgcaggg
     9421 gagcccgcgc cagttcggga actcggcccg gatcgcgtcg tggtgcagcg acgaggccgc
     9481 gcgggcgggt ttctcctccc gggtcttctt cctgcacgag ctcgacatcc gcccgtgcat
     9541 cgggtgttat gcctgctaca acttcggtta ctgcccgatc gacgacgata tgcccgggat
     9601 cgtccgggct ctcgagtccg cgtcggtcat cgtcgtctgc acgccggtct acaccggcac
     9661 cgtgccggcc ggcctgaagg ccgtgatcga ccgctgccag tggctccatg cccgggagaa
     9721 ggtgctgggg agggaggtgc acgccagggg cctgctcgtc gccgtcgcgg ggcagcgggg
     9781 gagcgagccg tttgtctgta taacccgcgt ggtcggcatg ttcatgggga acctgggcat
     9841 ccgcccggcc gccccggtgc tgatcgacga tctcgaccgg gcccgggacg tcgggaaggt
     9901 cgagggggcg gaagacgatg tccgggcggc gttgcgcgcg ctgctcgctc cccgggacga
     9961 agggtaatat acccctgccc cccaccgatg atgcatggca gaagtgactg tcgagattgt
    10021 cgggttcatg gaatccgaat gcggcccgtt tccctgtgac gagacgagat cctgcggcct
    10081 tgagacatgt tacccgtcaa acagcctgat agacgccatc ggcgccctgc gcgacgagct
    10141 cctcgcccaa tacgcggata gggtcgaagt gaagatgaca ctgctcgatg aggggatgcc
    10201 cgaccatatc aggaagatca tagaggagcg tcatccgccc ataccgatcg tcctcgtcaa
    10261 cgggcggctt acgtcgatcg ggagggtctc gctcgacctg ataaaagaag agattgagta
    10321 tgcgcttgaa gagacctaaa acatcttctt taactctccc gtctcaagat cgaagtacag
    10381 gccgtggagg cggacccgcc ctcctttttc cgcagccatg accggggggt aggtgcggag
    10441 gtgctcaagc tgcaggccga cgttctcgat ctcgatgagc cgcaaccggg tcttctcttc
    10501 ttcaggggtt ttcggcatct ggatcctgtc gtcgacacgg cgttttgctt ctcttgcgtt
    10561 gttgagccag agcgggatgt aggcgtcttt gccctcgtga tcgagcccct tgatggcgcc
    10621 gcagtccgag tgtccgcaga tgacgatatc cccgacctcg aggtggttga ttgcgtactc
    10681 aagcacggtc gcgaagttcc agtcatggac cgggacgatg ttgcctatgt tgcgctggac
    10741 aaagagttct ccggccctgg agccggtgat gcgttccggg tcgacgcgtg agtccgagca
    10801 ccctatccag agaaccgtcg ggtgctggcc agataccagc gaaccgtaat gttcaggcgc
    10861 tttgacaaaa tctctttcta gaaaccgttt gttaccctca agaaacttat ctatcattcg
    10921 tgatgctccg tagacgatag gggcggccat cctgatgggt gcgcctccaa aaatgggatt
    10981 gtcccccagt cttaaaaaga tatatacctg gaagttgcgg cgagaggcgc ggtgcggaat
    11041 ccggatagaa agggggattc caggttcgga tgatgcccta aagagcagat cggcccagga
    11101 tatcgacgat cggatcgggg tacggagaac catgtacatc agggcactga acagtgcatg
    11161 gtcgacaatt cggggttgtt tataacggat tgtcatccgt cgaatcgcaa taccgccggg
    11221 gtatatgcgc tcccgccagg ggagggagat ccctgcagga taccgttatg cgacgggatc
    11281 agaagaaacc ctgcgcccat cgggcgcggg aatcctcctc cccgaaaaaa agggttacct
    11341 ctggacggca ccgaggttcg cctgctcgac ggttcgcgcg taccgggcga gaacgcccgt
    11401 aagccggcgc tccgggggtt tccaggcccg cctccgctct tccagggttc cgggatcgac
    11461 caggaggtca aggctctttg cgtagaggtc gatagcgacc gcatctccgt cctcaaggag
    11521 ggcgatcggt ccgccgaccg cggcctcggg ggcgacgtgc ccgatgcacg ggccccgggt
    11581 gccgcccgag aagcggccgt cggtcaccag ggcgacccgg gcgtagccga gccccatcag
    11641 ggcggacgtc ggggcgagca tctcgggcat ccccggccca ccccggggtc cttcgtagcg
    11701 gatcacgacg acgtcgcctt ccactatatc gcggtggagg atcgcctcca tcgccgccgt
    11761 ttcgccgtca aagacacggg ccggcccccg gtgccgccac atcgccgccg ggacggcggc
    11821 gcatttgacg accgcgccgt cgggggcaag cgttccccgc acgaccttca ggccgccggc
    11881 agggttgacg gggtctttga cggtcctgat gatgtccggg tcggcgaccc gggcagtctt
    11941 cgcgatatcg aggatcgagc ggcccgagac cgtcggggcg tcgtcgaggt ggcccgcaag
    12001 catggcaagg accgcaggta tgcctcccgc ccggtagagg gtgagcatcg agtgcgggcc
    12061 gccgggctgc atgtggcaga tgtggggggt ctcctcgccg atggtgttga acgtctcaag
    12121 gtcgaggggg acgcctgcct cgcgggcgat ggccatcagg tggaggaccg tgttcgacga
    12181 tcccccgagc gccatatcca ctcgtatcgc gtttgcaagg cttgccggcg agacgatatc
    12241 ccgcgggcgg atgccctccc gtgcgaggcc gaccacccgc tccccgctct cccgggctat
    12301 gcggagtttt gccgcgtcga ccgccgggat tgccgcgcaa ccggggaggg agaggcccag
    12361 cgcctcggtc acgcacgcca tggtgtttgc ggtatagagg ccctggcagc tcccgcaccc
    12421 gggcattgcc gcgcactcga gttcgccgag ttcctcctcg ctcatcgtgc cggcggcgac
    12481 cctgcccacg ccctcga
//
LOCUS       DAOSEE010000060        12340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1979_length_12340_cov_216.099796, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000060 DAOSEE010000000
VERSION     DAOSEE010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 12340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12340
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1979_length_12340_cov_216.099796"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..420
                     /locus_tag="PLI76_06025"
     CDS             211..420
                     /locus_tag="PLI76_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone family protein"
                     /protein_id="HOI61625.1"
                     /translation="MVDIPLAPVGRIVKKSGAERVSSDANEELAKLMEQYASRIAKEA
                     IKLAGHAGRKTVKATDVRMAAETVK"
     gene            877..1134
                     /locus_tag="PLI76_06030"
     CDS             877..1134
                     /locus_tag="PLI76_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HOI61626.1"
                     /translation="MSKTFCRTLAKKKVMSNDGMLIGTIKNITVDLTTGQVIDLIVKP
                     DDTFKTDGYKMDGDKMLVPFEAVKDIKDYIVVDRYLLKRSG"
     gene            complement(1110..1814)
                     /locus_tag="PLI76_06035"
     CDS             complement(1110..1814)
                     /locus_tag="PLI76_06035"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HOI61627.1"
                     /translation="MLKALVTDVDGTLTDRRRRINTAAVEAIRTLVDAGIEVVLASGN
                     TVCFMDGLCRMIGTDGTIIGENGGVCRRGYKGSLHIAGDREICLEAFEVLHDHFAGQG
                     IDLELLSAQYRFADVALARNIDPDEARAVLRDHHLPVRVLDTGFAVHIQAPGVSKETA
                     MRKLAVDMGLSLDDMIAVGDSENDIEMLRAAGVGVAVQNAPELTRSAADWVSKSAYGD
                     GFVEAVKKYYPDRFSR"
     gene            complement(1808..2782)
                     /gene="radA"
                     /locus_tag="PLI76_06040"
     CDS             complement(1808..2782)
                     /gene="radA"
                     /locus_tag="PLI76_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair and recombination protein RadA"
                     /protein_id="HOI61628.1"
                     /translation="MTEIDLEDLPGVGPTTAEKLREAGYGTVESIATATTSDLAEAAE
                     IGEGTAKKVILAARKMADIGGFKTGRDILDKRKDVKKLSTLVPEFDELVGGGLETQAI
                     TEVYGEFGSGKSQLVHQLAVNAQLPEDLGGLHGSIIYVDTENTFRPERIEQMVNGLPE
                     GTDLGSMEEILERIHVARAHSSDHQMLLLETARELANQLRTSDYPVRLFVIDSLTSLF
                     RSEYAGRGTLAARQQKLNRHMHDLLKLLDDHNAVGLVTNQVMSNPAILFGDPTKPIGG
                     NIVGHTATFRLYLRKSKGGKRVARLVDSPNLPEGEAPFMVEQAGLKPC"
     gene            complement(2890..4176)
                     /locus_tag="PLI76_06045"
     CDS             complement(2890..4176)
                     /locus_tag="PLI76_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HOI61629.1"
                     /translation="MKFHYLLVDDLVGREEFERRVDAKVAESGDLLDERTAAMLVVRD
                     LDRAHVRIRDLSGTSSLACFFARVLSVEAPREFERSDGTTGIVANVMVGDETGRAKLA
                     LWDEKAGGVSEIGVGSVLEILGRPKGGGGRVVDVTAIAIQEAACDITCNEADTLAPAG
                     PAGDIEVRLIAVEAPRAFRRRDGSPGEMVEAVVGNEKGIFRLVAWVPETLLEAETGTN
                     VVIRGAVARESDRGIEYSLGEAGSVSPSDREIVIPMDTIAGIEEGKSYSIAGTVVSVQ
                     PSRSFVTKGGRPSSVRNLVIADSTGEVPVVIWGEKADGHLVSGDRIEAYNAAARRGRY
                     GDIELHLSWGSALVVLAGEEEEVDVRGTVIATGQGVALDTGDACYLLADPLPVGYDLR
                     VRGSLHRGVITVHHAEAVIPDPGDLQSRLDRFSGQP"
     gene            complement(4193..5485)
                     /gene="lysA"
                     /locus_tag="PLI76_06050"
     CDS             complement(4193..5485)
                     /gene="lysA"
                     /locus_tag="PLI76_06050"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOI61630.1"
                     /translation="MILPSHLSVRGGHLYIGEHDTVALAERFGTPLYVTSEDRVRENF
                     RRLSGALSAHYGKVRVLYAAKANGNLAVFRTLASMGAGSDVFSAGEVALALAAGMRPE
                     LLLFNGSSKSPADLALAVEKGIRVSVDSSDELRQLDRAAGAAGRTVDIAFRVNPAIDV
                     PTHPKIATGLATSKFGIPAEEILDAYREALAAEHVNPVGVHCHIGSQILAVEPFAREV
                     DVLIDLAADLLDIGVDLSFIDIGGGLGIPYRRETDRAPTPEEYAGAVMPVFLEGIRDL
                     GIEPELWVEPGRWLVADSTVLLSRVNSVKTAHKTFANVDAGFNLLIRPTMYDSYHEVV
                     AANRADAPAVREYSIAGPICETGDVLAKDRMLPELAAGDIVAMLDAGAYGFAMSSQYN
                     SRPRCAEVLLAGEKAALMRRAETIDDLTAPMATPPWQE"
     gene            complement(5482..6630)
                     /locus_tag="PLI76_06055"
     CDS             complement(5482..6630)
                     /locus_tag="PLI76_06055"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HOI61631.1"
                     /translation="MYARRMDHLPPYLFARIDDMKEAKQRQGVDVIDLGVGDPDLPTP
                     PHIVEALCAAARDPENHHYPSYAGMPAYREAVAEWYRGRFGVDLDQKKEVLALIGSKE
                     GIAHIAEAFVNPGEVVLAADPGYPVYKTSTLFAEGKVHELPIRAENDFLPVLEDIPAD
                     VVKQAKLLFINYPNNPTAATAPLSFFEEVVEFAREHDIIVVHDNAYSEITFDGYKAPS
                     FLEVDGAMDVGIEMHSLSKTYNMTGWRIGMACGNPDIVAGLGRVKTNVDSGAFNAIQH
                     AAITALTGPQDCVGRACAIYQERRDALVKGLAELGLDVKAPKATFYVWAPVDDCMAFA
                     AKLLDETGIVATPGVGFGKNGEGFVRFAITRSIERIHEAVDRMRGIDL"
     gene            6739..7938
                     /gene="nifS"
                     /locus_tag="PLI76_06060"
     CDS             6739..7938
                     /gene="nifS"
                     /locus_tag="PLI76_06060"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HOI61632.1"
                     /translation="MNDQAERPVYMDHAATTCMKPEVLAAMAPYFSEHFGNPSSLYRF
                     SRGSREGVEGARGEVAKALGAKPEEVFFTAGGTESDNWAIKGVAAAAKKKGGHIVTSA
                     IEHHAVLHTCEWLAKQGFSVTYLPVDGYGRVDPGDVENAITDRTVLVSVMTANNEIGT
                     VQPIGEIGRIAHDHGVLFHTDAVQAIGAVPIDVAAMEIDLLSLSGHKFYGPKGTGALY
                     IRQGTRIESLIHGGGQERGRRAGTENVPGIVGLGRAIEVATADIEGHNRRIAAMRDRL
                     IRGVLDTIPDTRLNGHPVERLANNANFSFRYVEGESILLLLDAHGICASTGSACSSGS
                     PEPSHVLLATGLTPEEAHGSLRLTLGDANTADDIDYALRLLPEVIGRLRRISPLTPAE
                     KRVQEAD"
     gene            7938..8366
                     /locus_tag="PLI76_06065"
     CDS             7938..8366
                     /locus_tag="PLI76_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOI61633.1"
                     /translation="MYTEKVIAEFTNPEYVGEIADADGVGEVGNPGCGDIMRISLKVE
                     GDRIVDARFKTFGCAAAIASSSMATRMIRGMTLSEAWNLSNADVVDALGGLPEPKVHC
                     SLLARGAIRAAINDYRTRRGLPPLEAGPSGCCEETEPGNG"
     gene            8392..8709
                     /gene="cutA"
                     /locus_tag="PLI76_06070"
     CDS             8392..8709
                     /gene="cutA"
                     /locus_tag="PLI76_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HOI61634.1"
                     /translation="MDLPEYVVVFCTAPAGEAEALARTLVDARLAACVNVTDVRSCYR
                     WEGKVHGEPEQLLIVKTQYRLLDPLVARIKALHSYETPEIIAIPIAGGYAPYLEWIRE
                     ETT"
     gene            8709..9458
                     /gene="fdhD"
                     /locus_tag="PLI76_06075"
     CDS             8709..9458
                     /gene="fdhD"
                     /locus_tag="PLI76_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HOI61635.1"
                     /translation="MEIVCRSGIQVTGEDVCAVRDDLIVEDRIRLFLNGEYLTTLVAS
                     PDRLDDLAAGFIICEGLADRVASVNVSGKDVYVTAPVGRDIRLEVESSGGYRVLGEPR
                     KVTSSITTTAETIRAVTAAIESDTWRRTGGVHCSVLFCDGELVTRACDVGRHNTVDKV
                     VGYAALRGLDRSTCILGCTGRQPAGMVAKAANAGIPIVVSRAASTDRGILTAERAGLT
                     LVCFSRGERFTIYTHPGRVSDVLAAIKKAQP"
     gene            9455..9928
                     /locus_tag="PLI76_06080"
     CDS             9455..9928
                     /locus_tag="PLI76_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1890 domain-containing protein"
                     /protein_id="HOI61636.1"
                     /translation="MTENDTRHALILLGCPQVPVQTSMALYLVHNLAQRGIRPVIAGT
                     PAARKLMEVADPGRHYLEEMTDIDAAIDEITEKRRDFDLCFVFIHNDSGVAYAGTMAY
                     ISEARVYALIFGEHAEDLAGEIEFPCEVVAAKATHNPMPLKRRLDEVMQWAVLRR"
     gene            9907..10179
                     /locus_tag="PLI76_06085"
     CDS             9907..10179
                     /locus_tag="PLI76_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1894 domain-containing protein"
                     /protein_id="HOI61637.1"
                     /translation="MGCLEALKPEILLSQTTFRDAREYIRKNIREYHEVEPGYRIFDV
                     HIIGVPPLYIGIEGGDLIFPYTKPCHGTFVVKVPGGEEIARLRARK"
     gene            complement(10232..10789)
                     /locus_tag="PLI76_06090"
     CDS             complement(10232..10789)
                     /locus_tag="PLI76_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOI61638.1"
                     /translation="MHTSSRSFLCICALGLLLITAGCTQNEPDTIVSAPETPGIRGPE
                     HYTANETLVAFVESAVDYARTHGKDKALVEFSDPNGSFVRGELYIYAYDFNNTVLAHP
                     FDPDKIGVNRMDELDAFGNPYTQQFLDAAKNGSGFVRFYYVNPAHNRTVESKLGYVMK
                     VDEDWWLGSGIYMGPADTGASRATP"
     gene            11092..>12340
                     /locus_tag="PLI76_06095"
     CDS             11092..>12340
                     /locus_tag="PLI76_06095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOI61639.1"
                     /translation="MSEPLRVLLIGESPGNLGEMLRTCGRKAELIHRERPRDGLALAS
                     GGEIDVVLLDLDLADGEGTAFLDRLRRTAPDIPVIVLTAAGEDRAAFHAMQSGAQDYL
                     VREKTDAELLCRAIRYAQERMRAEVLLRHSEAMYRRLIENLNEGIIAVDEAGLITFAN
                     PRMGEIFGCPARRLVGLPIAGFIAGENIPAAPNPFCRQTDQRQEFEQDLIRCDGGQVH
                     ALVATSPIVDAAGAPLGCLAGVLDITGRKQAEEALRQSEQRYRLVVESLSEGIWVIDR
                     NACTSFVNPRMAEMLGYSPGEMIGRSVHSFLPASGWAAMQDRLKRRRDGTREQYECKF
                     TRKGGETITVLVISSPFVDDAGEFQGSIVGVMDITERKRVEEEIRVRNEQLMVLNQVI
                     SVSATSLSLAELLEESLEKTLNLM"
BASE COUNT         2171 a         3872 c         4093 g         2204 t
ORIGIN      
        1 gagggcggca agcccgtcgg tatcgttacc cagaccgata ttattcgggt ctttccctca
       61 ctttgatacc ctccccgttc cgaatttcct gcttcaaccc ctctggaagg ggataagtat
      121 ttatatatga atacctatgg tgcttctgta aaacgagctc tgtttgagag tacatttcgc
      181 agagctcaat gaaaaggggg aaatgagatc atggtagata tacctctggc acctgttggc
      241 agaatcgtga agaagtccgg cgcggaacga gtgagttctg atgccaatga agagctcgca
      301 aaactcatgg agcagtacgc atcgcgaatc gcaaaagagg cgatcaagct cgcgggacac
      361 gcaggccgaa agacggtcaa ggcaaccgat gtccggatgg cggccgagac cgtaaagtaa
      421 accaatcccg actccatttt tccccgggac aacctgttgt gccggttatt attgctgcaa
      481 gaactgctct acctgccggg gtgcccgcct ttgcccctgc agggataccc gtacgccgtt
      541 ctccgggaac tcccacccct tcccgggcgg ggcagcgttc gcgatcattc gatgggaagc
      601 cctgcccggt ccttctccgg ggatctcccc gtggagatat cgcggagggg catgaacctc
      661 acccctgcgg ggctcaaggt taccgtcatg ccggccctca cgaacggttg cggcaccgca
      721 cctccggggt gagagcgggg ggacggagac cgggactgcc ggggggcagg gcggttgtgg
      781 tggccggcct ctccgggcag cgatcctgga aagccatttc tgaagagttt tgagggattt
      841 agaatattta tatagtacaa acaccccacg ctctctatga gcaaaacctt ctgccgcacc
      901 cttgccaaga agaaagtgat gagtaatgac ggcatgctca tcgggaccat caagaacatc
      961 acggtcgacc tcactacagg gcaggtcatc gacctgatcg tcaaaccgga tgatacgttc
     1021 aagaccgatg gctacaagat ggatggagat aagatgctcg tgccgttcga ggcggtgaaa
     1081 gacataaaag attatatcgt ggtcgaccgc tacctgctga agcgatcggg gtaatacttt
     1141 tttacggctt ccacaaaccc atccccgtat gcgctcttcg agacccagtc cgccgccgac
     1201 cgggtcagtt ccggcgcgtt ctgcaccgcg acgccaaccc cggcagccct gagcatctcg
     1261 atatcgttct cggaatcccc gacggctatc atatcatcaa gggatagtcc catatccacg
     1321 gcgagttttc gcatcgccgt ctccttgctc acgccgggtg cctggatgtg gacggcaaac
     1381 cccgtgtcca gcacccggac aggaagatgg tggtcacgga ggaccgcccg tgcctcgtcg
     1441 gggtcgatgt tccgggcaag ggccacgtcg gcaaaccggt actgtgcgct gagcagctcg
     1501 agatcgatgc cctgcccggc aaaatgatcg tgtaagacct cgaacgcctc aaggcagatc
     1561 tcccggtcgc cggcgatatg gagggagcct ttataccccc tccggcagac gccgccgttc
     1621 tcgccgatga tggtcccgtc cgtcccgatc atcctgcaga gcccgtccat gaaacagacg
     1681 gtgttgccgc ttgcaagcac cacctcgata cccgcatcga cgagcgtccg gatggcctcg
     1741 acggcagcgg tgttgatccg ccgccgccgg tcggtgaggg tgccgtcgac gtccgttacg
     1801 agcgccttca gcacggcttg agccccgcct gctcgaccat gaacggcgcc tcgccctcgg
     1861 ggaggttggg gctgtccaca aggcgggcaa ccctcttgcc gcccttgctc ttgcgcaggt
     1921 agaggcggaa ggttgcggtg tgcccgacga tgttgccgcc gatcggtttt gtcgggtcgc
     1981 caaagaggat ggcagggttc gacatcacct ggttggtcac gaggccgacc gcgttgtggt
     2041 cgtcgagcag cttcaagagg tcgtgcatgt gccggttcag tttctgctga cgggcggcga
     2101 gggtcccgcg ccctgcgtac tccgagcgga agagggacgt cagcgagtcg atgacgaaga
     2161 gcctgaccgg atagtcggat gtccgcaact ggtttgcgag ttcccgtgcg gtctcgagga
     2221 gcagcatctg gtggtcggag ctgtgtgccc tggcgacgtg gatgcgctcg aggatctcct
     2281 ccatcgaacc gaggtccgta ccctccggga ggccgttgac catctgctcg atacgctccg
     2341 ggcggaacgt gttctcggtg tcgacgtata tgatgctccc gtgcagcccg ccaaggtctt
     2401 cggggagctg ggcgttgacg gccagctggt ggacgagctg gctcttcccg gacccgaact
     2461 ccccgtacac ctcggttatc gcctgcgtct caagaccgcc gccgaccagt tcgtcgaact
     2521 cgggaaccag ggtgctgagt ttcttgacgt cttttctctt gtccagtata tcccggcccg
     2581 tcttgaaccc gccgatatcc gccatcttcc gtgcggcaag gatcaccttc ttcgcggtcc
     2641 cctcgccgat ctcggccgcc tcggcgagat ccgacgtggt ggccgttgca atgctctcga
     2701 cggtcccgta gcccgcctcg cggagttttt cggcggtggt cggcccgacg cccggtaaat
     2761 cttcaagatc aatctctgtc atgtcaatcc tcacttgatc tctgcggttg tttgttatgt
     2821 tacacttaga gtgttgaacg ggagacataa aggtcgcttg ccatgcgggg tgaaagtgaa
     2881 agtcagggat cagggttgcc cggagaaccg gtccaggcgg ctctgcagat ctcccggatc
     2941 ggggatcaca gcctcggcgt ggtggacggt gatcacgccc cggtgcaggc taccccggac
     3001 gcgcagatcg tacccgaccg gcaacgggtc ggcaaggagg tagcaggcgt cgccggtatc
     3061 aagagcgacc ccctgcccgg tggcgatgac cgtccccctg acatccacct cctcctcttc
     3121 cccggcaagg acgacgaggg cgcttcccca ggagaggtgc agttcgatat cgccataccg
     3181 cccccggcgg gcggccgcgt tgtaggcctc gatacggtcg ccggatacca ggtgcccgtc
     3241 cgccttctcc ccccatatga cgaccgggac ctcgccggtc gaatcggcga tgaccaggtt
     3301 ccggacggac gacggcctcc cgcccttcgt gacgaacgac cgggacggct ggacggatac
     3361 gaccgtcccg gcgatcgagt acgacttacc ttcctcgatg cccgcgatcg tatccatcgg
     3421 gatgacgatc tcccgatcgg agggcgagac ggatcctgcc tcgccgaggc tgtactcgat
     3481 gccgcggtcg ctctcccgcg cgacggcgcc ccggatgacg acgttcgttc ccgtttccgc
     3541 ctcaagaagg gtctccggga cccaggcgac gagccggaag atgccctttt cgttcccgac
     3601 gaccgcctcg accatctccc ccgggctccc gtcgcgtctc ctgaacgccc gcggggcttc
     3661 gacggcgatc agcctgacct cgatgtcgcc tgccggcccg gcgggcgcga gggtgtcggc
     3721 ctcgttgcac gtgatgtcgc acgctgcttc ctggatggcg atcgcggtga cgtcgacgac
     3781 tcttcccccg ccgcctttcg gccggccgag tatctcgagc acgctcccga cgccgatctc
     3841 cgagacgccg ccggccttct cgtcccagag ggcgagcttc gcccttccgg tctcgtcgcc
     3901 gaccatcacg ttcgcgacga tgcccgtcgt gccgtcggac cgctcgaact cccggggcgc
     3961 ctcgaccgag agaaccctgg caaagaaaca cgcaaggctt gaggttccgg aaaggtcgcg
     4021 tatccggacg tgcgcccggt cgaggtctct gacgacgagc atcgccgccg tccgctcatc
     4081 gaggaggtcg ccggactccg ccaccttcgc atcgacccgg cgctcgaact cctctctccc
     4141 cacgaggtcg tcgaccagga ggtagtgaaa cttcacggtc gccgctccgg ccctactcct
     4201 gccagggcgg cgtcgccatc ggggcggtca ggtcgtcgat cgtctcggcc cggcgcatca
     4261 gggccgcctt ctcgcccgcg aggagcacct cggcgcaccg ggggcggctg ttgtactgcg
     4321 acgacatggc aaacccgtat gccccggcat ccagcatcgc gacgatgtcg ccggcggcaa
     4381 gttcggggag catcctgtct tttgcgagga cgtcgcccgt ctcgcatatc ggccccgcga
     4441 tcgagtactc cctcactgcg ggtgcgtccg ccctgtttgc cgcgacgacc tcgtggtagg
     4501 agtcgtacat cgtcgggcga atgaggaggt tgaacccggc gtcgacgttg gcaaacgtct
     4561 tatgagcggt cttgaccgag ttgacccggg agaggaggac cgtcgagtcg gcgacgagcc
     4621 accggccggg ttcgacccag agttcgggct cgatcccgag gtcacggatg ccttcgagga
     4681 agaccggcat caccgctccc gcgtactcct ccggcgtcgg ggcgcggtcg gtctcccgcc
     4741 ggtaggggat gccgaggccg ccgccgatgt cgatgaacga gaggtcgacc ccgatatcga
     4801 ggagatcggc cgcaagatcg atcagcacat cgacctcacg ggcgaagggc tccacggcga
     4861 ggatctgcga gccgatgtgg cagtggaccc cgaccgggtt cacgtgctcg gcggcgagcg
     4921 cctcgcggta ggcatcgagg atctcctctg ccgggatgcc gaacttgctc gtcgcaaggc
     4981 cggtcgcgat cttcgggtgg gtggggacgt cgatcgcggg gttgacccgg aatgcgatgt
     5041 cgacggttct tcctgccgct cccgccgcgc ggtcgagctg ccggagctcg tcggacgagt
     5101 cgaccgagac ccggatgccc ttctcgaccg cgagggcgag gtcggcgggg gatttcgagc
     5161 tcccgttgaa gaggagcaac tccgggcgca taccggcggc aagggcaagc gcgacctcgc
     5221 cggcggagaa gacgtccgat cccgcgccca tagacgcgag tgtccggaat accgcaaggt
     5281 tgccgttcgc ctttgcggcg tagaggacgc ggacctttcc gtagtgggcg ctgagcgctc
     5341 ccgagaggcg ccggaagttc tcgcggaccc ggtcctcgct cgtgacgtag agcggggtgc
     5401 cgaaccgctc cgcgagggcc accgtgtcgt gctcgcctat gtagaggtgc ccgcccctga
     5461 ccgagaggtg ggaggggagg atcacaggtc gatcccccgc atccggtcga ccgcctcgtg
     5521 gatacgctcg atcgaccggg tgatggcgaa ccggacgaac ccttcgccgt ttttgccgaa
     5581 gccgactccg ggggtcgcga cgatcccggt ctcgtcgagg agcttcgcgg caaacgccat
     5641 gcagtcgtcg accggcgccc agacgtagaa ggtggccttc ggcgccttga cgtcaagccc
     5701 gagctcggcg agccccttca ccagggcgtc gcggcgctcc tggtagatcg cgcacgctcg
     5761 cccgacgcag tcctgggggc cggtgagcgc cgttatcgcc gcgtgctgga tggcgttgaa
     5821 cgcgccggag tcgacgttcg tcttgacccg gccgagcccg gcgacgatgt cggggttgcc
     5881 gcaggccatg ccgatccgcc acccggtcat gttgtaggtc ttggagaggg agtgcatctc
     5941 gataccgaca tccatcgcgc cgtcgacctc gaggaacgag ggcgccttgt agccgtcgaa
     6001 ggtgatctcg gagtaggcgt tgtcgtggac gacgatgatg tcgtgctccc gcgcgaactc
     6061 gacgacctct tcgaagaacg agaggggcgc cgtcgccgcg gtggggttgt ttgggtagtt
     6121 gatgaagagc agtttcgcct gcttcacgac atcggcgggg atatcctcga ggaccgggag
     6181 gaagtcgttt tcggcccgta tggggagctc gtggacttta ccctcggcaa agagggtgga
     6241 tgtcttgtag accgggtagc cggggtcggc ggcaaggacg acctcgcccg ggttgacgaa
     6301 agcctcggca atgtgcgcga tgccttcctt tgacccgatg agggcgagca cctccttctt
     6361 ctggtccagg tcgacaccga acctgccccg gtaccattcg gccaccgcct cgcggtaggc
     6421 gggcataccg gcgtaggagg ggtagtggtg gttttccggg tcgcgcgccg cggcacagag
     6481 cgcttccacg atgtgcgggg gtgtggggag gtcggggtcg ccgacgccga ggtcgatgac
     6541 gtcgactccc tggcgctgtt ttgcctcttt catatcgtcg atgcgggcaa agaggtaggg
     6601 ggggaggtgg tccatgcgtc ttgcgtacat taccagatct tacgcacgga accctatgta
     6661 attatccgcg accgggcctc cccgtggctc ccggccggca acgctgatac gcttccagcg
     6721 cgtacgctgt acctggacat gaacgaccag gcagagcgcc ccgtgtacat ggatcacgcg
     6781 gcgacgacat gcatgaagcc cgaggtcctc gcggcgatgg ccccgtactt ctcggagcac
     6841 ttcggcaacc cctcatccct ctaccggttc tcccgcgggt cccgcgaggg cgtggagggg
     6901 gcgaggggag aggtggcgaa ggctctcggg gcaaagcccg aggaagtctt cttcaccgcc
     6961 ggcggcacgg aatccgacaa ctgggcgatc aagggggtgg ccgccgccgc aaagaagaag
     7021 ggcggccata tcgtcacctc cgcgatcgag caccacgccg tcctccatac ctgcgagtgg
     7081 ctcgcgaagc aggggttctc ggtcacctac ctcccggtcg acgggtacgg ccgggtggac
     7141 cctggcgacg tcgagaacgc gatcaccgac cggacggtcc tcgtctcggt gatgacggca
     7201 aacaacgaga tcgggacggt ccagccgatc ggggagatcg gccggatagc gcacgaccac
     7261 ggcgtcctct ttcataccga cgccgtccag gctatcgggg cggtcccgat cgacgtggct
     7321 gctatggaga tcgacctcct ctcgctctcc gggcacaagt tctacggccc gaaggggacc
     7381 ggggcgctct acatacgtca gggaacccgt atcgagagcc tgatccacgg cggagggcag
     7441 gagaggggcc ggcgggccgg gaccgagaac gttcccggga tcgtcgggct cgggcgcgcg
     7501 atcgaggttg cgaccgccga tatcgagggg cacaaccgcc ggatcgccgc gatgcgggac
     7561 cggctgatcc gcggggtcct cgataccatc ccggataccc ggctgaacgg ccacccggtc
     7621 gaacggctcg cgaacaatgc gaacttcagt ttccgctacg tcgaggggga gtctatcctg
     7681 ctcctgctcg acgcccacgg tatatgcgca tcgaccggga gcgcctgctc gtcggggtcg
     7741 cctgagccct cgcacgtcct gctcgcgacg gggcttaccc ccgaagaggc gcacgggtcg
     7801 ctccggctga cgctcggcga tgccaatacg gcggatgata tcgattatgc cctccgtctg
     7861 ctgccggagg tgatcgggcg gctgcggcgg atatcgccgc tgacgcctgc agagaagaga
     7921 gtgcaggagg ccgactgatg tatacggaga aagtgattgc agagttcacg aacccggagt
     7981 acgtgggcga gatagcggat gccgacggtg tcggcgaggt cgggaacccg ggttgcgggg
     8041 atatcatgcg gatctccctc aaggtcgagg gagaccggat cgtggatgcc cggttcaaga
     8101 cgtttgggtg cgcagcggcg atcgcatcga gttcgatggc gacccggatg atccggggga
     8161 tgaccctctc cgaggcatgg aacctctcga acgccgacgt ggtggatgct ctcggcggcc
     8221 tcccggagcc gaaggtgcac tgctcgctcc ttgcccgcgg cgccatccgg gcagcgatca
     8281 acgactaccg gacccgccgg ggacttcccc ccctggaggc gggaccctcc gggtgctgcg
     8341 aggaaaccga gcccggtaat ggttaaatac aggcggtacg aagttaaaac catggatctc
     8401 ccggaatatg ttgtggtctt ctgcacggca ccggccgggg aggcggaagc gcttgcacgt
     8461 acgctcgtcg atgcccggct cgcggcctgc gtgaacgtca ccgatgtgcg gtcctgctac
     8521 cggtgggaag gtaaggtcca cggcgagccc gagcaactcc tgatcgtcaa gacgcagtac
     8581 cgcctgctcg accccctcgt cgcccggatc aaggcgctcc acagttacga gacccccgag
     8641 atcatcgcca tacccatcgc cggcggctac gctccctacc tcgagtggat acgggaggag
     8701 acgacctgat ggagatcgtc tgccgctccg gtatccaggt gacgggcgag gatgtgtgcg
     8761 ccgtccgcga cgacctcatc gtcgaggacc gcatccggct cttcctgaac ggcgagtacc
     8821 tcacgaccct ggtggcgagc cccgaccgcc tcgacgacct cgcggccggg ttcatcatat
     8881 gcgaggggct ggccgaccgg gtcgcttcgg tgaacgtctc cggaaaggat gtgtatgtca
     8941 cggccccggt cgggcgggac atccgtctcg aggtggagtc gtccgggggg taccgcgtgc
     9001 tcggcgaacc gcggaaggtc acctcgtcga tcaccaccac ggcggagacc atccgggcgg
     9061 tcacggcggc gatcgagtcc gatacctggc ggaggaccgg cggcgtccac tgttcggtcc
     9121 tcttctgcga cggcgaactc gtcacccggg cctgcgatgt ggggaggcac aacaccgtcg
     9181 ataaggtcgt gggctacgcg gccctccggg ggctggaccg gtcgacctgc atcctcgggt
     9241 gcacggggag gcagccggcg gggatggtcg cgaaggcggc gaacgcgggc atcccgatcg
     9301 tcgtctcccg cgccgcctcg accgaccggg gcatcctcac ggcggagcgg gcggggctca
     9361 ccctggtctg cttctcgcgg ggggagcggt tcaccatcta cacgcaccct gggcgggtgt
     9421 ccgacgtcct tgccgcgata aagaaggctc aaccatgacg gagaacgata ccagacacgc
     9481 actgattctg ctcgggtgcc cgcaggtgcc cgtccagacg agcatggcgc tctacctggt
     9541 gcacaacctc gcgcagcggg gcatccggcc ggtgatcgcc gggacgccgg ctgcgcggaa
     9601 actgatggag gtggccgacc cgggccggca ctacctggag gagatgaccg atatcgatgc
     9661 cgcgatcgat gagatcacgg aaaaacggcg ggacttcgat ctctgcttcg tcttcatcca
     9721 caacgattcg ggggtcgcgt atgccgggac gatggcgtat atatcagagg cgcgggtgta
     9781 tgcgctcatc ttcggggagc atgcggagga ccttgccggc gagattgagt tcccctgcga
     9841 ggtcgtggcg gcgaaggcca cccataaccc catgccgctg aagcggcgac tcgacgaggt
     9901 gatgcaatgg gctgtcttga ggcgctgaaa cccgagatcc tcctctccca gacgacgttt
     9961 cgggacgcga gagagtacat caggaagaac atacgggagt accacgaggt cgaaccgggc
    10021 taccggatat tcgacgtcca catcatcggc gtgccgccgc tgtatatcgg catcgagggc
    10081 ggggacctga tctttcccta caccaagccc tgccacggga cgttcgtggt gaaggtcccc
    10141 ggcggcgagg agatcgcgag gctgcgggcg agaaaatagt atttttctct tctatctctt
    10201 ccaaaaatgt gaatgcggtc cggatggtca tctacggcgt agcccggctg gctcccgtat
    10261 ctgccggtcc catgtagatc ccggagccga gccaccagtc ttcgtccacc ttcatcacgt
    10321 agcccagttt ggactcgacc gtccgattgt gcgcggggtt gacgtagtag aaccggacga
    10381 acccggagcc gttctttgcc gcatcgagga actgttgcgt gtaagggttg ccgaaggcgt
    10441 cgagttcgtc catccggttg acgccgatct tgtcggggtc gaacggatgg gcaaggacgg
    10501 tgttgttgaa gtcgtaggcg tagatgtaga gctcgccccg gacgaacgaa ccgttcgggt
    10561 cggagaactc aacaagggct ttatccttgc cgtgcgtcct ggcgtagtcg acagcgctct
    10621 cgacgaaggc gacgagcgtc tcgtttgcgg tgtagtgctc cggtccccgg atgccgggag
    10681 tttccggcgc cgaaacgatc gtgtcgggct cgttctgtgt acatcccgcg gtgatgagga
    10741 gcaagccgag ggcgcagatg cagagaaacg atctggaaga cgtatgcata tgattgagtc
    10801 ggtattcatc ccctaaaaat gcatgccgag gacaaccggc gccttatggg cccttccggc
    10861 cgcgggactg agcgctgccg ggagcccggc gatgggcact gccgctgccg gagcatctcc
    10921 ggtgtgcggg aacccatgca ggagagacgc ccaaagaaag aggtataaat tcaccgaagg
    10981 cggtactaca gtagtgagaa cccggtcgcc tgcggtcatg cccgcgatgg ccgggagagc
    11041 gaagtttacg ccgatacggg caggtaccgg gccctgaatg cgagtgatac gatgagcgaa
    11101 ccgttgagag tgctgttgat cggggagagt cccggcaatc tcggggagat gcttcgcacc
    11161 tgcgggcgca aggctgagtt gatccaccgt gaacgccccc gggacgggct tgccctggca
    11221 tcggggggcg agatcgacgt cgtgctcctc gatctcgatc tcgcggacgg agaggggacc
    11281 gcctttctcg accgcctgcg ccggacggct cccgatatcc cggtcatcgt gctcactgcg
    11341 gcgggagaag accgcgccgc gttccatgcg atgcagagcg gcgcgcagga ttacctggtc
    11401 cgggagaaga ccgacgcgga actgctctgc cgggcgatcc ggtacgcgca ggagcggatg
    11461 cgggcggagg ttttgctccg gcactccgag gcgatgtacc ggaggctgat cgagaacctc
    11521 aacgagggca tcatcgccgt ggacgaggcc ggcctcatca ccttcgccaa cccgcgtatg
    11581 ggggagatct tcggctgccc cgcaaggcgc cttgtcgggc tgccgatcgc cggtttcatc
    11641 gccggcgaga acatcccggc cgccccaaat ccgttctgcc ggcagaccga ccagagacag
    11701 gagttcgaac aggacctcat ccgctgtgac ggcgggcagg tccacgcgct cgtggccacg
    11761 tcgccgatcg tggatgccgc cggcgcccct ctggggtgcc tcgcaggggt gctcgacatc
    11821 accggccgca agcaggccga ggaggccctc aggcagagcg agcagaggta tcgactggtc
    11881 gtggagagcc tcagcgaggg gatatgggtg atcgaccgga acgcctgcac gtcgtttgtc
    11941 aacccccgga tggcggagat gctcggctac tccccgggag agatgatcgg gagatccgtc
    12001 cattcgttcc tccccgcctc cggctgggct gcgatgcagg accgcctgaa gcgtcgacgg
    12061 gacggaacca gagagcagta cgagtgcaag ttcacccgga aggggggcga gaccatcacc
    12121 gtgctggtga tatcgtcgcc gttcgtagac gacgccgggg agttccaggg ctcgatcgtc
    12181 ggcgtgatgg acatcaccga acgaaagagg gtggaagagg agatccgggt cagaaacgag
    12241 cagctcatgg tcctcaacca ggtgatcagc gtctcggcca cctcgctctc ccttgccgag
    12301 ctcctggagg agtcgcttga gaagacgctc aacctcatgg
//
LOCUS       DAOSEE010000061        12335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1981_length_12335_cov_227.200651, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000061 DAOSEE010000000
VERSION     DAOSEE010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 12335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12335
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1981_length_12335_cov_227.200651"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..488)
                     /locus_tag="PLI76_06100"
     CDS             complement(108..488)
                     /locus_tag="PLI76_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61640.1"
                     /translation="MALSYRDATINSVEANPTGSGSTGGGRDESGKVYFFPNISVSIG
                     YWFDNEGIPNSDWNTHFTAKLFVNDSLIETKQVLSGGGPQTYSFFAYKFPAPGTYKVE
                     VRGKNSKSLEVTIKNPPVQEKKAA"
     gene            1085..1348
                     /locus_tag="PLI76_06105"
                     /pseudo
     CDS             1085..1348
                     /locus_tag="PLI76_06105"
                     /inference="COORDINATES: protein motif:HMM:NF019997.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
     gene            1946..2710
                     /locus_tag="PLI76_06110"
     CDS             1946..2710
                     /locus_tag="PLI76_06110"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HOI61641.1"
                     /translation="MTIQVMGIHGLPIIHAGDDLAALICERTAFEDGDILCIASSVSA
                     KANGQTRNLTEIEPTERAIAIAAAAGEDPRFIQVILDASVDVLLESPFILAAVPCGHI
                     GVRAGVDRSNVEDDIAIILPPDPMAAAAGIREDIRRIAGRTVAVILTDTCGRAFRRGQ
                     TGHAVGWSGVTAIRDFRGDTDLFGRPLTITEEAIVDEIAGFANIVMGESSNGVPAVVV
                     RGLPAWQGHDRLYFRPEEDVMRRALATSRQNLKSAP"
     gene            complement(2699..2863)
                     /locus_tag="PLI76_06115"
     CDS             complement(2699..2863)
                     /locus_tag="PLI76_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61642.1"
                     /translation="MAACPCCGVELTGYERVAVFGDKEKVRTETVLCCPCCRWQGVCR
                     ENCPTDLLWG"
     gene            complement(2871..4634)
                     /locus_tag="PLI76_06120"
     CDS             complement(2871..4634)
                     /locus_tag="PLI76_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HOI61643.1"
                     /translation="MTSHARHLPEILACEADRVQSFGSEYASYADQLRELARRLDSGR
                     FHLAVLGQFKRGKSTLINALLGESLLPSSVIPLTAIPTLIAYGDRQSIRIRFQGNRDD
                     EVVEGGTPHDLYRSLLHSVSEEFNPKNERGVLQVEVTHPAPLLAEGVVLIDTPGIGSI
                     HQHNTEMTLNFLAQCDAALFLVSADPPITEVEVEFLRTIRDAVTKVFFVLNKVDYLTD
                     DEQKTALAFFETVLRERAGIQGPVTIFPVSAKRGLAAATAEDAALWQSSGMAKVSDHL
                     VGFLAGEKNAVLRDAVQKKARAVLGAVALRLDLSRRALEMPLDDLEGRMRTFEVKIRE
                     AELQRRHAADILEGDHKRIAISLEEEMRALRERSLARLMGVAEGALACPAGPDETAAQ
                     NAVAAAIPPLYEHELGETTATIEREVTALLQTHQQRAGDLIGSIRTAAADLFEIPYHA
                     RENDRMLSMRQEPYWVSRKGWESTFSPVTNGLVDRALPRSMRERRIRARMEKQISYLV
                     VRNTENLRWATIQNVDATFRQFGRELDADLAEAIDATKGAITAAYTKRKEHASGTAED
                     LARLQKASAEVASAISSLEQE"
     regulatory      complement(4682..4743)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            4959..5027
                     /locus_tag="PLI76_06125"
                     /pseudo
     CDS             4959..5027
                     /locus_tag="PLI76_06125"
                     /inference="COORDINATES: protein motif:HMM:NF015884.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5215..5490)
                     /locus_tag="PLI76_06130"
     CDS             complement(5215..5490)
                     /locus_tag="PLI76_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61644.1"
                     /translation="METRTINEHQEMLPTTVVEGGGRVVLDDSPEEPGRLYSEVVADP
                     DISVVVAFIPCKRFEQPGGQGRNTRRRPETGWSVFRRPPSSGPVLKA"
     gene            complement(5501..5893)
                     /locus_tag="PLI76_06135"
     CDS             complement(5501..5893)
                     /locus_tag="PLI76_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61645.1"
                     /translation="MDRSEGVREASRTGRPQVGTVTLPHTGNDRALTVRCIPLPERSG
                     SVTAILVVFDDEDARRGENGPDAACCESARELEEHLRYLASGSRVPIAAIDPEDPLAS
                     AKRSYNAALETVERMFAVSGLFGHRPDR"
     gene            complement(6052..7047)
                     /locus_tag="PLI76_06140"
     CDS             complement(6052..7047)
                     /locus_tag="PLI76_06140"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI61646.1"
                     /translation="MYAVEAAGVSRRFGRRVAVEGLTLQINPGEIVGFLGRNGAGKTT
                     TLRMLAGLLHPDEGTVRVFGYDTQARGFAAHGLVGVLPEDAAIRVMGTAWENLLQAAE
                     SRYVRPEEASRRGRELFALLGLDDRADEPVRGFSKGMKRRLAIAMSVINDPLLLVLDE
                     PTSGLDIESVVVIRDVIRELNRRGATILLTTHTLEEADALCDRVIILDRGRLVACDTP
                     ARLKASVPHEPSLVVTLLPDAPGVADEIQRIPAITAVSGDGGRYRVGYTDLPGVLEAL
                     ATYSRRTNRTIAALQTSVPSLEEVFLHVTGSDLCSRPVASVCWTDPARIDKEGGA"
     gene            complement(7035..7406)
                     /locus_tag="PLI76_06145"
     CDS             complement(7035..7406)
                     /locus_tag="PLI76_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOI61647.1"
                     /translation="MEIYFCPNCWRWGPTVQEHCPACGYALADYHALSYDERLVRAVR
                     HPIQENRMIAVELLGKRRYAPAVAVFADLLATERDYYTIREVARALRRIDTPESRAVL
                     DRLRYHSSPLVRALLEEPCTQ"
     regulatory      complement(7529..7602)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     regulatory      complement(7656..7720)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(7783..8982)
                     /locus_tag="PLI76_06150"
     CDS             complement(7783..8982)
                     /locus_tag="PLI76_06150"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI61648.1"
                     /translation="MRDDGDGWLNKTVIGAGIASFFSDLGHEAVTVLLPSLLIVIGAP
                     VYALGVIEGLSDGVSSFVKLFSGHWSDQLGKRKEFALTGYLATAVFPAFVAVATVWPV
                     VLLGRVIGWAGRGTRGPPRDAILAKSVPPHDLGKAFGFHRAGDTLGAIAGPLMALALV
                     SSIGLREIFWLTLIPGFLAVATFWLLVREKNPTPSRDKTKLVTSLRGLPGAFKRFLGA
                     VLVFGTADFSHTLLIAFAIVALTPSLGLAGATAVGAGLYLVRNVVYAAASYPFGVLGD
                     RFGRVKMLILGYTVAVATFIGFAVVPPDLVGYGALFALAGLFIAAEDTLEGAVAAELV
                     EEQRRGLGFGVLATVNGIGDLASSIIIGFLWAFVGYAAGFLFAAIVAAAGTLLLVATR
                     SLPSAAG"
     gene            complement(9049..9690)
                     /locus_tag="PLI76_06155"
     CDS             complement(9049..9690)
                     /locus_tag="PLI76_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HOI61649.1"
                     /translation="MDRWFSVSGLATGLIRPGDAIADRLIAAAPRPGGLVDGDCIVVA
                     ESAVATAEGRLVRLDGVKPTPVAHDLARRYTMDPRIVEIILQESDAVIGGMPGLLLCR
                     AHGTLLPNAGVDASNAPPGCVVLLPANPGASAAGIRDAIRASAGVEVAVIVADSRPYP
                     MRSGCAGIALGCAGMAAIRHGRAVADCIASTAELVMGEADEGIPAAIVRWWKP"
     gene            complement(9696..9875)
                     /locus_tag="PLI76_06160"
     CDS             complement(9696..9875)
                     /locus_tag="PLI76_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61650.1"
                     /translation="MASMIEDLKLAVMGRLDQVCAGIAAGGDPEEELARAARDIEALC
                     DRADQLYRDAVSSMW"
     gene            complement(9883..11175)
                     /gene="eno"
                     /locus_tag="PLI76_06165"
     CDS             complement(9883..11175)
                     /gene="eno"
                     /locus_tag="PLI76_06165"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOI61651.1"
                     /translation="MTEIDTTIRSIRAREILDSRGNPTVEAEVTLACGAHARAASPSG
                     ASTGAHEAIELRDGAPGRYNGKGVQRPVNLINYGIGDRLTGFDARGQAAVDRALIELD
                     GTPNKRNLGANTTLAVSMAVARAAAATEGRWLWEYLSNPAEPLLPVPMMNILNGGVHA
                     NWQGPDFQEYMIAPYGAPTFAEALRWGAEIYHALKAFLKEKGYSTGVGDEGGFAPAVS
                     SNTEPLDLIVEAIERAGYAPGRDIGIALDPASSAFYSNGVYNLRTEGLTLTAAEMVDR
                     YRDLAGAYPIVVIEDGLAEDDWDGWRLLTEAIGDRVELVGDDLFVTNVTRIERGIREG
                     VANAVLIKPNQIGTVTETIAAVRLARANGWGAMVSHRSGETVDTFIADFTVAMRTGHL
                     KTGAPARGERVEKYNQLLRIEEAAGDAAHYAGRAGFVR"
     gene            complement(11162..12019)
                     /locus_tag="PLI76_06170"
     CDS             complement(11162..12019)
                     /locus_tag="PLI76_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HOI61652.1"
                     /translation="MISNVERNQTIRFIADRRCASGGYCHYRLDEPNAADTFFALDIL
                     RLLGALAPDPVTDEFLLRMQHPDGRYPTFYAGAYVLRCLALIGKEPAVDPCPWLRSFD
                     PFRGSGERPIESISVFERTCTHLGLYRTLGIHPDEQLRDVVLRHLIAYHNPDGGFGRP
                     RSSLIETAHAVTILAMVGKPHGTFGAERFLRQCEDAAFGYLNTPGATPAYLEHLAAGV
                     ECAALTGRPPRFPERCRTFIYECRNANGGYSRSIYGGISTLENTHLAIRTLALINQIE
                     HRRDQHDRD"
     regulatory      complement(12026..12087)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(12253..12315)
                     /locus_tag="PLI76_06175"
                     /pseudo
     CDS             complement(12253..12315)
                     /locus_tag="PLI76_06175"
                     /inference="COORDINATES: protein motif:HMM:NF018933.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2139 a         4001 c         3934 g         2261 t
ORIGIN      
        1 cacctgttcg gtggggactt cccgctccgc ccccttcacc ctcgcaagtt aaaatggcgg
       61 ggattagccc tgtcctgatc ctaaaaagcc gacctttccc cgcgccgcta cgccgccttc
      121 ttctcctgca ccggggggtt cttgatcgta acctctagcg acttcgagtt cttgccgcgg
      181 acctccactt tgtacgtgcc cggcgccggg aacttgtagg caaagaacga gtacgtctgc
      241 ggccctccgc ccgagagcac ctgcttcgtc tcgatcagcg agtcgttgac gaacaacttc
      301 gcggtgaaat gcgtgttcca gtccgagtta gggatacctt cgttgtcgaa ccagtagccg
      361 atgctcaccg atatattcgg gaagaaatac accttgccgc tctcgtcgcg accgccgccg
      421 gtactcccgc tgcccgtagg atttgcctct acggagttta tcgttgcatc acggtacgat
      481 agtgccatgg gcctacctgt ataaaagtga tataataaat ttccgctcga cctgttgcag
      541 agaacccttc gaatcggaag aggttccgaa ctgacggcat gcttcccgac gtgactgtcc
      601 ccgttgctct atcgggctgc tctttttgac tctccctccc tttttccgct cctatctctc
      661 gttttagcag cgttgttccg gcgcccggat cggcgaactc ctcaacctca gcagaggcca
      721 cgcccggttc gaccgttacg tcacggcggc gactgtgaac ggcaagaccg gggcgcgacg
      781 gttctgcccg atcagccccg tgccgggatc aatatgcatc cactgcgtgc gaatgcaaac
      841 gccctgcttt ttgacctccc gaagccttgc gctctgcgtg cagcaggctg cggaggaggg
      901 cgaacgttac ggcaacggga ggttcttctg atgccgtccc tcactctctc cgctttttgg
      961 tgtgacgatg acgagcagga cgccccggat ttcgtcgtcc tcgtgtcacc ctacgaggcc
     1021 ggccctcggg ccatgctggt ccgtcgtcgg aggggagacg agtgataacg accacctgcg
     1081 accgatgcgg cgccgtcgtc ggggaagagt gctgccggac gcaccaggag cgggagaact
     1141 tctggggagc gccctgctcc tcctctccgg ccacacctgg cggggcacct acaaccagac
     1201 ccggcgggcg ggcggccatg accggcgcgg ggagaaggcg agcggcatgg tggtcttctg
     1261 gtcccgctac gatacaaaga aggtcacgaa cgagaacggc gagcaggtga cggtggcgct
     1321 ccagcgcgac cggccgctga tccgctacta caaggccttc aacctctcgc agtgcgaggg
     1381 catcgcaggc gaggtccggc cggcattacc cctccctgcc gtcacccact gtttcgtcga
     1441 gatcccggtc gagccgcccg agaaggtcgt gcagctcccc ggcgatacct tcgagttctg
     1501 ccgtcatccc cccggaaacc tcgccatacg cccgcacgtg cttcggccgc agctcttccg
     1561 ctgcgatatc gatcgcgatg agcgaacccc tgatggcgcg cgacaccggg actgctggcc
     1621 gatccctggc gatgccctgg tcgtccagga tgcggacgag ccgggtgcga agacacgcga
     1681 caaaatcctc gatcgtttgc cgctgcacgg gggtaagatc ggaagagtag cgcgggaaca
     1741 cactcttcga tgccgaagcg ttcagaacgc cttcgatatc ggtcaggagt cggtcgatat
     1801 actcacaggt gctcaggata tatcgcacct ggtgctcgtt cggggacggc gcccccggtt
     1861 cggtgccctc attcattctt gcctctctgc tatgcgttac gatctttcag cgacccttat
     1921 ccccaccggc gatgaattcc tgatgatgac aatccaggtc atgggcattc acggcctccc
     1981 gatcatccac gccggtgacg atcttgccgc actcatctgc gaacggactg cctttgagga
     2041 cggcgacatc ctctgcatcg cctcgtcggt ctcggcgaaa gcgaacgggc agacccgcaa
     2101 cctcacagag atcgagccga ccgagcgggc gatagcgatc gcggcagccg ccggcgagga
     2161 tccgcggttc atccaggtca tcctcgacgc ctccgtcgac gtgctcctcg agagcccgtt
     2221 catcctcgct gccgtcccct gcgggcatat cggcgtccgg gcgggcgtcg accggagcaa
     2281 cgtggaggat gatatcgcca tcatcctgcc gcccgacccg atggcagccg ccgccgggat
     2341 ccgggaggat atccgcagga tcgccggcag aacggtcgcg gtcatcctca ccgacacctg
     2401 cgggcgggcg ttccgccggg ggcagactgg ccacgccgtc ggctggagcg gggtaacggc
     2461 gattcgcgat ttccggggtg atacggacct cttcggccgt cctctcacca tcaccgagga
     2521 ggcgatcgtc gacgagatcg ccgggttcgc gaacatcgtc atgggggaga gcagcaacgg
     2581 cgtcccggcc gtcgtcgtcc ggggccttcc cgcctggcag gggcacgacc ggctctactt
     2641 ccgccctgaa gaggacgtca tgcggcgggc gctggcaacg tcgcggcaga acctaaaatc
     2701 agccccatag gagatcggtc gggcagttct cccggcatac cccctgccac cggcagcagg
     2761 ggcagcagag aacggtctcg gtcctgactt tctccttatc tccaaagacg gcgacccgct
     2821 cgtagccggt cagttccacc ccgcagcacg gacatgcagc catcttctct tcactcctgc
     2881 tcaaggctcg atatagcgga cgcaacttct gccgacgcct tctgcaaccg ggcgaggtct
     2941 tccgccgtcc cgctcgcgtg ttctttcctc tttgtgtatg cggcggttat ggcacccttc
     3001 gtggcatcga tcgcttccgc gagatccgca tccagttcgc ggccgaactg tcgaaacgtc
     3061 gcgtcgacgt tctggatcgt cgcccaccgg agattttcgg tgttccggac cacgaggtag
     3121 gagatctgct tctccatccg tgcccggatc cgcctctccc gcatcgagcg cggcagcgcc
     3181 cggtcgacga gaccgttcgt gaccgggctg aaggtgctct cccagccctt ccgggagacc
     3241 cagtacggct cctgccgcat cgagagcatc cggtcgttct cccgggcgtg gtaggggatc
     3301 tcgaagaggt ccgcggcggc ggtccggatg gacccgatga ggtcgccggc ccgctgctgg
     3361 tgagtctgca ggagcgcggt cacctcccgc tcgatcgtcg cggtcgtttc gccgagttcg
     3421 tgctcgtaga gcggcgggat ggcggcggcc acggcgttct gcgccgccgt ctcgtccggg
     3481 cccgcggggc aggcgagcgc gccctccgcg acccccatga ggcgcgcgag cgaccgctcc
     3541 cggagcgccc gcatctcctc ttcgagcgag atcgctattc tcttatggtc gccttccagg
     3601 atatcggcgg catgccggcg ctggagctcc gcctcgcgga tcttcacttc gaacgtccgc
     3661 atccggcctt cgaggtcgtc aagcggcatc tccagcgccc gccgggagag gtcgagccgg
     3721 agcgcgacgg cgcccagcac ggcccgggcc ttcttctgga ccgcatcgcg gagcacggcg
     3781 ttcttctcgc cggcgaggaa cccgaccagg tggtccgaga ccttcgccat cccgctcgac
     3841 tgccagaggg cggcatcctc ggccgttgcg gccgcaagcc cccgtttcgc cgagaccggg
     3901 aagatcgtga ccggcccctg gatacccgcc cgctcgcgga gcaccgtctc gaagaacgcg
     3961 agcgcggtct tttgctcgtc gtcggtgagg tagtcgacct tgttgaggac gaagaagacc
     4021 ttcgtgaccg cgtcgcggat ggtccggagg aactccacct cgacctcggt gatcggcggg
     4081 tcggcggaga ccaggaagag cgccgcatcg cactgggcga ggaagtttaa ggtcatctcg
     4141 gtgttgtgct ggtggatgga cccgatcccg ggcgtgtcga tcaggacgac accctctgcg
     4201 aggagcgggg cggggtgggt cacctccacc tgcaacacgc cccgctcatt cttcggattg
     4261 aactcctccg agaccgagtg gaggagcgag cggtacaggt cgtggggtgt gccgccctcg
     4321 acaacctcgt cgtcgcggtt gccctggaac cggatacgga tgctctgcct gtcgccgtag
     4381 gcgatcagcg tggggatggc ggtcagcggg atcaccgagc tcgggaggag tgactcgcca
     4441 aggagcgcgt tgatcagcgt gctcttgccc cgcttgaact ggccgagcac ggcgaggtgg
     4501 aaccggccgg aatcaagccg gcgagccagt tcccggagct ggtcggcgta ggaagcatac
     4561 tcggaaccga acgattgaac acggtcggct tcgcaggcaa ggatttcggg caggtgccgg
     4621 gcgtggctgg tcatgggatc tctcctgaca aatggatatg gatcgggcgg gcactggccg
     4681 cacgatggta ggagttatca gccacaaaag cggttagagg tgaaccccat cacctgaatc
     4741 tgtatcattg tatcttgctt cgcagataat taatgctttg ttctccgata gctccactcc
     4801 cctgatgcgt tctgcccccc cggtaccggg caggactata tgtagagagg gcagaaatcc
     4861 ttctcgtaat atcggatcca gccgtttcaa cactcttcaa taggatcgac ctgaataacg
     4921 tgcggttttt acgcctccag ttcaccgatg ttctcggcat gcccaggacc gtcgccatcc
     4981 ccgccgacca ggcagagaaa cccctgacca ccggcatcgg gtttgacggg tcctcgaaag
     5041 agtccgacat gctccccaaa ccggacctct ccgcctacgc ccttctcgtg gctgccgcac
     5101 gaggatgcag tcgcccggtt cgtttgcaac gtgaccggac ccggtggccg gcccttcgca
     5161 ggcgacccgt gctgcgtgct gcggcggggc gatggacgac gtgtcaaagg acggctacgc
     5221 cttcaacacc gggccggaac tgggtggtcg acggaaaacc gaccatccag tttcagggcg
     5281 ccggcgggta tttcgacctt gcccccccgg ctgttcgaac ctcttgcagg ggatgaaggc
     5341 gaccacaacg gagatatcgg ggtctgcgac gacttcgctg tagagccggc ctggttcttc
     5401 cggcgaatcg tcgaggacca ctcttcctcc accctcgaca acggtcgtcg ggagcatctc
     5461 ctgatgctcg ttgattgttc tcgtttccat acgcaccacc tcatctgtcc ggccggtgcc
     5521 cgaacagccc ggataccgca aacattcgtt cgacggtttc aagcgccgcg ttgtaggacc
     5581 tcttggcgct cgcaagcggg tcctcaggat cgatcgccgc tatcggcacc cggctcccgg
     5641 acgccaggta ccggaggtgt tcttccagtt cacgggcgct ctcgcagcat gcggcatcag
     5701 ggccattctc gcctctccgg gcatcttcat catcgaatac gacgaggatc gcggtgaccg
     5761 acccggatcg ctccgggagc gggatgcacc ttacggtgag ggcccgatcg ttgccagtgt
     5821 gcggaagggt gaccgtgccg acctgtggcc ggcccgtccg gctcgcctct ctcacccctt
     5881 cgctccgatc caatgcccgg ccgatttcgc catcttgatc gctaaaccgg ctccggtatg
     5941 ctctgttcac ggccaggacg cgggcacccg ggagcacggc tgccgccggc gtggagatgc
     6001 actcacagag cgcttcaagg gaattacgca tcatttcgac tgttttctgc ttcatgctcc
     6061 cccctcctta tcgatcctcg ccgggtcggt ccagcagacg gatgctaccg gccttgagca
     6121 aagatctgac ccggtgacgt ggaggaagac ctcttcgagg ctcggaacgc tcgtctgcag
     6181 ggcagcgata gttcggttgg tccgccggga gtatgtagca agcgcctcca gcacaccggg
     6241 caggtccgtg taaccgacac gataccgccc gccgtccccg gaaactgccg tgatcgcagg
     6301 gatgcgctgg atctcgtctg ccacgcccgg tgcatccggc aggagagtga ccacgagaga
     6361 cggttcgtgc ggcacgcttg ctttcagccg tgccggggta tcgcatgcga caagccgccc
     6421 ccggtcgagg atgatcaccc ggtcgcagag ggcatccgct tcctcaaggg tgtgcgtcgt
     6481 cagcagaatc gtggcgcccc gccggttgag ttcccggatg acgtcgcgga tgacgacgac
     6541 actctcgatg tccagaccgg acgtcggttc gtcaagcacc agcagaagcg ggtcgttgat
     6601 gacgctcatg gcaatcgcaa gccgccgttt catgcccttt gagaacccgc gcaccggctc
     6661 gtcggcccgg tcgtcgagcc cgagcagcgc gaagagctcc cggccccgcc gacttgcttc
     6721 ttccggacga acgtaacggc tctccgccgc ttgcaacagg ttctcccacg ctgtccccat
     6781 cacccggatc gcggcatcct cgggcagcac cccgacgagc ccgtgtgcag cgaacccccg
     6841 cgcctgcgta tcgtatccaa agactcgtac cgtgccttcg tccgggtgca ggagtcctgc
     6901 gagcatccgg agcgtggtcg tcttcccggc accgttccgg ccgaggaacc cgacgatctc
     6961 gccgggattg atctgcaggg tcaatccttc gaccgcgacc cgtcgcccga accgccggga
     7021 gacgccggcc gcttctactg cgtacacggt tcctccagca gggcacgcac cagcggcgag
     7081 gagtgatagc ggagccggtc gagcaccgca cggctctccg gggtatctat cctacgcagt
     7141 gcccgtgcga cttcgcgaat tgtgtagtag tcccgttctg ttgccagcag atcggcaaat
     7201 accgctactg cgggtgcgta tcggcgtttt ccaagaagtt cgacggcgat catgcgattt
     7261 tcctggatag ggtgacggac ggcccgtacc agccgctcgt cataggagag cgcatggtag
     7321 tcggcgagtg catacccgca ggccgggcag tgctcctgga cggtcggacc ccagcgccag
     7381 caattcgggc agaagtagat ctccaccgca cctcactctc cctggtggag cgggtaggtc
     7441 acggcgctcc cttgaatccc gtcgcctatc aggtactgaa aggctgctcc gataccaata
     7501 agccatatct gcatgagtgg tagacctccg tgtagcagac atcatcagcg cagagggcgg
     7561 ttttggcgtg gtgccggggt ggacgtcatc acccgatctt gaaatcgggc cgtgcagcgg
     7621 tgttccatcc atcggtgata tatgcccgca cggcctcaag aatagaagtc atcagcccgg
     7681 tggagggcgg tttgcggcgg acttcatcac cggagtacta tccactctga aatgagactg
     7741 caagggatta atcctttctg gctgcggcac ggataccgct cctcatccgg ccgcagaagg
     7801 caaggaccgt gttgcaacca ggagcagcgt cccggcggca gcgacgatcg cagcgaacag
     7861 gaacccggca gcatacccga caaacgccca gagaaacccg atgataatac ttgatgccag
     7921 atcgccgata ccgttgacgg tggcgaggac gccgaacccg agacctcgac gctgttcttc
     7981 tacgagttct gccgcgaccg caccttcaag ggtatcctcc gccgcgataa acagaccggc
     8041 cagcgcgaag agtgcaccat agccgaccag atccggcgga accacggcga acccgatgaa
     8101 tgtcgcgacc gcgaccgtat acccgaggat aagcattttc actcgtccga accggtcacc
     8161 gaggacgccg aacgggtagc tggccgccgc gtagaccaca tttctgacga ggtagaggcc
     8221 ggcgccgact gcggtcgcac ccgccagtcc gagcgagggt gtcaaggcga cgattgcaaa
     8281 ggcgatgagg agcgtatgtg agaaatcggc ggtcccgaag accaggaccg ccccgaggaa
     8341 gcgtttgaag gcaccgggaa ggccgcgaag cgaagtgaca agcttagttt tgtcgcggga
     8401 cggcgtcggg tttttctccc ggacgaggag ccagaaggtc gcgacggcaa gaaaacccgg
     8461 gatgagggtg agccagaaga tctcccgcag gccgatcgag gaaaccaggg ccagcgccat
     8521 aagcgggccg gcgatggcgc cgagtgtatc gccggcccgg tggaacccga aggccttgcc
     8581 gaggtcgtgc ggcgggacgg attttgccag gatcgcgtcg cggggcggcc ctcgagtgcc
     8641 gcggcccgcc catccgatca cgcggccgag gaggacgacc ggccagacgg tcgcaacagc
     8701 aacaaaggca gggaagactg cggtggcgag gtaaccggtg agcgcgaact ccttcctctt
     8761 cccgagctgg tccgaccagt gaccggagaa gagtttgacg aagctcgaaa caccgtcgct
     8821 caacccttcg atcaccccga gcgcatagac gggagctccg atgacgatca ggagggaggg
     8881 aagcaggact gtgacggctt cgtgaccgag gtcggagaag aaactcgcta tgcctgcacc
     8941 gatcaccgtc ttgttcagcc agccgtcgcc gtcatcgcgc attgttccct cccttttgca
     9001 ggatccggcc ctccgggtca cacgttcatt tccctgccgc cgcctcattc acggtttcca
     9061 ccaccggacg attgccgcag ggatgccttc gtcggcctcg cccatgacga gttccgcggt
     9121 ggatgcgata cagtccgcca ccgcccggcc gtgcctgatc gccgccatcc cggcacaccc
     9181 gagggcgatg ccggcacagc ccgaccgcat ggggtaaggg cggctgtcgg cgacgatgac
     9241 ggcgacctca accccggcag acgcccggat ggcatcccgg atcccggcgg cgctcgcccc
     9301 cgggttggca gggaggagga ccacgcatcc cggcggagcg ttggacgcat ccacgccggc
     9361 gttgggcaga agcgtgccgt gagcccggca gaggaggagc cccggcatgc cgccgatgac
     9421 ggcatcgctc tcctgcagga tgatctcgac tatccgcggg tccatcgtgt accggcgggc
     9481 aaggtcatga gccaccgggg tgggttttac cccgtcgagc cggacaagcc tgccttcggc
     9541 ggtggccacc gccgattcgg cgacaacgat gcaatctccg tcgactaagc cgcccggccg
     9601 gggcgctgcc gcaatgagcc ggtcggcgat cgcgtctccc ggccggataa ggccggttgc
     9661 gagaccggaa acggagaacc acctgtccac gccgctcacc acatcgagga gacggcgtcg
     9721 cggtagagct ggtcagctct gtcgcagagc gcttcgatgt cccgtgcagc ccgggcgagt
     9781 tcctcttccg gatcgccgcc ggcggcgatt ccggcacaga cctggtccag ccgccccatg
     9841 acggccagtt tgaggtcctc gatcatcgat gccattgcta ccctaccgga cgaacccggc
     9901 ccgcccggcg taatgggcgg catctcccgc cgcttcttcg atccggagga gctggttgta
     9961 cttctccacc cgctcgcccc gggcgggcgc tcccgtcttg aggtggccgg tccgcatggc
    10021 gacggtgaag tcggcgatga acgtatcgac cgtctcgccg ctccggtggg agaccatcgc
    10081 gccccagccg tttgcccggg caagccgaac cgcggcgatc gtctcggtga cggtgccgat
    10141 ctggtttggc ttgatcagga cggcgttcgc cacgccttcc cggatcccgc gctcgatccg
    10201 tgtaacattc gtcacgaaga ggtcatcgcc gacgagttcc acccggtcgc cgatcgcttc
    10261 ggtcagcaag cgccagccgt cccagtcatc ttcggcaagc ccgtcctcga tgacgacgat
    10321 ggggtaggcg ccggcgaggt cgcggtagcg gtcgaccatc tccgcggcag tcagggtcag
    10381 gccttcggtc ctgagattgt agacgccgtt tgagtagaac gcgctcgatg ccgggtcgag
    10441 ggcgatcccg atatccctgc cgggggcgta gcccgcccgt tcgatcgcct cgacgatcag
    10501 gtcgagcggc tcggtgttcg aggagaccgc gggcgcaaac ccgccttcgt cgccgacgcc
    10561 ggtgctgtaa cctttctctt tgaggaaggc tttgagggcg tggtagatct cggcgcccca
    10621 ccggagcgcc tcggcaaacg tcggggcgcc gtagggggcg atcatgtact cctggaaatc
    10681 aggaccctgc cagttcgcgt gcacgccccc gttgaggatg ttcatcatcg ggaccgggag
    10741 gagcggctct gccgggttgc tcaggtactc ccagagccag cggccttctg tagcggcggc
    10801 cgcacgggcg acggccatcg agacggcaag cgtcgtgttg gcgccgaggt tccgcttgtt
    10861 cggcgttccg tcgagctcga tgagcgcccg gtccaccgcc gcctggccgc gggcatcaaa
    10921 cccggtcagg cggtcgccga tgccgtagtt gatcaggttg acgggccgct gcacgccctt
    10981 cccattgtaa cggccgggtg cgccgtcccg gagttcgatc gcctcgtgag cgccggtgga
    11041 tgccccggag ggggatgcgg cgcgtgcgtg cgccccacag gcgagggtaa cttccgcttc
    11101 gacggtcggg ttgccccggg agtcgaggat ctcccgggcg cggatcgacc gtatggttgt
    11161 gtcaatctcg gtcatgctgg tctctcctat gttcgatctg gtttatgagg gcgagtgtcc
    11221 ggatcgccag gtgagtgttt tcaagcgttg agatcccgcc gtagatcgag cgggagtaac
    11281 cgccgttggc gttccggcac tcgtagatga acgtccggca cctctccggg aaccgagggg
    11341 ggcggccggt gagggcggca cactccacgc cggcggcgag gtgttcgagg tacgcgggcg
    11401 tcgctccagg ggtgttcagg tatccgaatg ccgcatcttc gcactgccgg aggaaccgtt
    11461 cagccccgaa cgtgccgtga ggctttccaa ccatagcaag gatcgtcacg gcgtgagcgg
    11521 tctcgatgag cgacgagcgt gggcggccga acccgccgtc cgggttgtgg tatgcgatca
    11581 ggtgacggag cacgacatcc cgaagttgct cgtccggatg gattccgagg gtccggtaga
    11641 ggccgaggtg tgtgcaggtc cgctcgaaga cggagatcga ttcgatgggg cgctcgccgg
    11701 atccccggaa agggtcaaac gaccggagcc aggggcaggg gtcgacggcc ggctcttttc
    11761 cgatgagcgc aaggcaccgg agcacgtagg cacctgcata gaacgtaggg tatctcccgt
    11821 ccgggtgctg catccggagc agaaattcgt cggtgaccgg gtcgggtgcg agggcgccca
    11881 ggagccggag gatgtcgagc gcgaagaagg tgtcggcggc gttgggttcg tccagccggt
    11941 agtggcagta gccgccgctt gcgcaccggc ggtcggcgat aaaccggatc gtttggttcc
    12001 gttcgacgtt tgagatcata gagggcaggg ggtagaagtc atcagccgtg tcggcggttg
    12061 gaggcggact tcatcgcctg catctgatcg tatgacgttt ggaaagtgta tgcaaggatt
    12121 gtggtgaact accccgcgcc ttttgggcgg ggcttcctgt ttcatggcca acacttgcac
    12181 caccgagatg tgatgtagag gtcttggctc cacaggctcg aagggctgtt ccatccccac
    12241 acggtgaata ttcaccgcag cattgtagtc tcgatcggca acaaacccgc aataggggca
    12301 atcgtgaact ctcaccgaga gatccttctt cacga
//
LOCUS       DAOSEE010000062        12274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_1993_length_12274_cov_259.665030, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000062 DAOSEE010000000
VERSION     DAOSEE010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 12274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12274
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1993_length_12274_cov_259.665030"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..519)
                     /locus_tag="PLI76_06180"
     CDS             complement(10..519)
                     /locus_tag="PLI76_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HOI61653.1"
                     /translation="MIEIYQNLYVGTQEDYEVTVEAHETWCVVHACRSPYHCLAVTCS
                     PVGTVPEDHPEHLVARRGNRLMLNLVDTHNPDDVPKEAIDAALLFIDRCLAKGRPVLV
                     HCGFGISRSAAIGLLYLAAHTDVLPVESLAAAEAAYRRIYPAYRPGRGIRGFLAAHWD
                     EYAKRRVTA"
     gene            complement(516..1448)
                     /locus_tag="PLI76_06185"
     CDS             complement(516..1448)
                     /locus_tag="PLI76_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOI61654.1"
                     /translation="MTTVIAVDLGGTNLRAALIDSADATILAHTAAPTPASGPSGDAI
                     TAAIIDRIETLLASAPGENATAIGVASAGPLDLDRGWVVNSPNIAFPVVEIVEPLSER
                     FDLPVALINDARAGVLGEWWAGAARGSDNVVYVTLSTGIGGGAVVNGRLLLGATGNAG
                     EIGHIPVDTRYNLTCGCGYKGHWEAYASAKNLPGFFAAWRKEADVRDVAFDAGSTRAI
                     LEAVRKEDPVALAFMEALGQVNARGVSAVIVAYNPDIIVFDGPLARYHGDIVIRHMEP
                     YIDRYLALPRLAVSELGGRSPLLGAAAYALEVRR"
     gene            complement(1525..1767)
                     /locus_tag="PLI76_06190"
     CDS             complement(1525..1767)
                     /locus_tag="PLI76_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HOI61655.1"
                     /translation="MSQPSAGSSAGGIRSIGYDPTTKALLVICESGDAYRYAGVRKEV
                     YESLLAAPAREQFVRESLEGRYPREKYPCMAVGEGI"
     gene            complement(1823..2035)
                     /locus_tag="PLI76_06195"
     CDS             complement(1823..2035)
                     /locus_tag="PLI76_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HOI61656.1"
                     /translation="MQRQAVESTNIRSVGYNEEDEILEVEFHSGGVYNYVGVPPEVYE
                     GLLAARSKGRYFGDFIRLRYPYEKVR"
     gene            complement(2174..2407)
                     /locus_tag="PLI76_06200"
     CDS             complement(2174..2407)
                     /locus_tag="PLI76_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61657.1"
                     /translation="MMPADRGWGCVLFVLPALLLVAIASAAGVADPAGDFLDPDITAL
                     SCYIREGILYVRTDLAREPVGSYGGCDLCRRRP"
     gene            complement(3006..3326)
                     /locus_tag="PLI76_06205"
     CDS             complement(3006..3326)
                     /locus_tag="PLI76_06205"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOI61658.1"
                     /translation="MHPSFRTALDAFLAKAIYRWGDRIEEVILFGSTARGEATPSSDL
                     DLLVITNEEDFRLRRSLIGLAYDIMLETGVNLSVKVLSRGDLERRRNSPFLKAVPAEG
                     ARVG"
     gene            3390..3656
                     /locus_tag="PLI76_06210"
     CDS             3390..3656
                     /locus_tag="PLI76_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61659.1"
                     /translation="MHDHYSTRGRKAMGGRLGITWKEFDLEQFRRGMVVELEHGLHDP
                     VTNVTDDDLFLTAKTALAHLNEFPDNYDRLEEQAEAYRAERRAA"
     gene            3739..3948
                     /locus_tag="PLI76_06215"
     CDS             3739..3948
                     /locus_tag="PLI76_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61660.1"
                     /translation="MERRGRAEWIKIRQRRVIDEKEYYADRLYETRRDATSYGRMLQN
                     QDKDVLILGTIHEGGHRGYAVFVRR"
     gene            complement(3959..4540)
                     /locus_tag="PLI76_06220"
     CDS             complement(3959..4540)
                     /locus_tag="PLI76_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOI61661.1"
                     /translation="MTGQPILVTDRLLLRPFTLADASMVQRLAGEYAVASSALEIPYP
                     YPDGVAEAWIATHRPGFERGVHAIYAVTCDGDLVGAAGLVEIDRDHRRAELGYWMGRP
                     YWGRGYATEATRAVIEYGFSVLGLHRVYAMHFSRNRASGRVMEKCGMVHEAHLRGHVR
                     KWGVVEDIDVCGVLHGEWRERCTGPAIRECRAG"
     gene            complement(4537..5082)
                     /locus_tag="PLI76_06225"
     CDS             complement(4537..5082)
                     /locus_tag="PLI76_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOI61662.1"
                     /translation="MENQPILATDRLLLRSFALADAFTVQQLAGDYAVAATTLLPHPY
                     PDGLAELWIASLREGIERGDTAAFAITLAGEGRLIGGVRLRIDCGHRRAELGFWVARS
                     CWGRGYATEAVRAMIEYGFSALGLHRVYAMHFSRNPASGRVMEKCGMVHEGHFREHIR
                     KWGVFEDVDIWGILREGGAFP"
     gene            5364..6389
                     /locus_tag="PLI76_06230"
     CDS             5364..6389
                     /locus_tag="PLI76_06230"
                     /EC_number="1.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HOI61663.1"
                     /translation="MIKVAINGYGTIGKRVADAVAKQPDMEVVGVSKTSVSAEAFVAK
                     KRGYPLYIADIGKKAAFEKAGLDVAGDVEAMLKAADIVVDATPGGVGEKNRPVYERLG
                     KKAIYQGGEEHEVAGFSFNAHVNYKEAEGHQFARIVSCNSTGLVRIISAMDQAFGVDR
                     VRAVMVRRASDPGDVKRGPVDATVLNPATIPSHHGPDVNTVLPDVNIVTLAMIVPTTF
                     MHMHAIQMDLKKETTREEVLKVFENHSRIGLVRKATGIKSNAQLREYAQDLGRPRADV
                     WENGVFEESVSVLGGKEFYCFQAIHQEADVIPENIDCIRALMGTVKDPEESIRMTNKA
                     LGFVAIR"
     gene            6444..7391
                     /locus_tag="PLI76_06235"
     CDS             6444..7391
                     /locus_tag="PLI76_06235"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOI61664.1"
                     /translation="MDPYELATRNTVEVVTDEELRALIDRPVRRVYTGYEPSGEIHLG
                     HMVTVNKLMDLQKAGFEVTVLIADLHAFLNRKGTMDEIREIAKYNRRCFEGLGLVGAK
                     YVLGSDVQLTPEYELAVLELSQAITLNRAKRSMDEVGRQMDNPTVSQMVYPIMQMVDI
                     ATLKVDAAVGGIDQRKIHMLAREHLPSIGYPAPVCIHTPIINGLDGKKMSSSAGNVIS
                     VADAAEDIRQKMKKAFCPPEVENNPVLEILRYHIFPRAGTVTIRRPAKFGGDREFAAY
                     NDLERAYAAGEIHPLDLKNAAANHLIDILAPVHDYVCSR"
     gene            7399..8175
                     /locus_tag="PLI76_06240"
     CDS             7399..8175
                     /locus_tag="PLI76_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase RIO"
                     /protein_id="HOI61665.1"
                     /translation="MGHDDMHERFDREIEAMGVRIKDADALKVRDDVFDEVTLVALYK
                     LVHKKLISVIGGPISTGKEANVFYGERDKRGIAIKIYRIQTANFKAMTEYLAGDRRFA
                     SIRGSRKGIVFAWTKKEYSNLARAHEVGIPVPEPLAFDRNILLMEFLGREEVPYPQLR
                     NAEVADYGVTYGEILGYIERLYQEARLVHADLSEYNILYYEKPYVIDMGQAVTLDHPR
                     ASAFLIRDIRNINRYFSRYCDVQDEQEIIGAVTGSRGRER"
     gene            8197..8739
                     /locus_tag="PLI76_06245"
     CDS             8197..8739
                     /locus_tag="PLI76_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOI61666.1"
                     /translation="MTRQELKVTSARLGVLIGKSGSTKREIEEKTGTILRIDSEEGMV
                     TIEAEDPVAVMTTTNVVSAINRGFSPERAYRLLEDEDMMFDVIDLADLSGTTRHLERL
                     RGRIIGKAGTSRAQIEDMTGTELSVHGKTVAIIGLPDQVETARKAVEMLIQGVPHEHV
                     YAFLDRKKKEAKQAMLEYYS"
     gene            8822..10960
                     /locus_tag="PLI76_06250"
     CDS             8822..10960
                     /locus_tag="PLI76_06250"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOI61667.1"
                     /translation="MEIAGLPIPPDLLADYTGRGITELYPPQAACVEAGLFAGKNLLI
                     AIPTASGKTLVAEMAMHHQVGRGGKCLYIVPLRALASEKFEEFSGKGLRVGIATGDFD
                     RRDEYLGRNDIIVATSEKVDSLLRNRTPWLAEVTLLVLDEVHLIDDPSRGATLEMVIA
                     KLRHKNPAIQIIALSATIGNPGDLAGWLGAELVESDWRPVDLREGVFFWNGIRFADRD
                     REVERRSKYEDLDLVLDTVAEGGQCLVFVSSRKNAEAFAKRAASGLKLKNPILAGYAD
                     KLRSSASTDMGKTLAACVAQGAAFHHAGLAREERQIVEAGFREGQIRVIASTPTLAAG
                     LNLPARRVIVRDYLRFNAGEGMVPIPVREYRQMAGRAGRPHLDPYGEAVLIAKSEEMV
                     DELFDCYINAPAEDVRSRCADEAVLCTHILSLIATGFAREADEVFAFMDGTFYAHQGE
                     SPRALSRAVHRALEFLREAEMITEVGEWIEATEYGSLVSRLYIDPRSAEVIVAAMIDR
                     QEYTDIGFLHLLCSTPDMPTLYIRRDDMYLLDRFLADHRDDLWTDIPWDAGEGFDRSL
                     KTALLLADWADEVGEDTICERYRVGPGDIYGMVESVAWLVHAARSLAKLFAPHLAAPI
                     EEMELRTKHGIKKELLPLIRLHGIGRVRARRLFNNNLGSIEALRAAGPEKVGKILGQG
                     IAARIFEQLEGTREEVGEVSEEQSTLSRFG"
     gene            10967..11539
                     /gene="cgi121"
                     /locus_tag="PLI76_06255"
     CDS             10967..11539
                     /gene="cgi121"
                     /locus_tag="PLI76_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Cgi121"
                     /protein_id="HOI61668.1"
                     /translation="MVEMTETVKTGEMVETAETVEAACDIFPAVFAVDENIRFLRRIR
                     RIADEHRTHIILFDADRLAGRAHVEAALRHAWRSWAGGNPIANSIEMEALLYAAGTRQ
                     CQVAASFGIHPGLNRSYIAVCPSAPGIRDHLAGLVTFVDGEHDETIDPGKRARLADLF
                     GITPEEVAVVGEDRFRDLVIERVALLDVYR"
     gene            complement(11530..12027)
                     /locus_tag="PLI76_06260"
     CDS             complement(11530..12027)
                     /locus_tag="PLI76_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HOI61669.1"
                     /translation="MESNHLKPLTVTGAVITVSSSRKPGTDASGKALIDLLSADGIEV
                     THYAVVPDDVARIRAEVSAAISRATCVIVTGGTGLTPDDVTIEAVAPLLAKTIDGFGE
                     LFRLKSYEEIGTAAILSRAIAGVIDGRAVFCIPGSTKAVTLAAREIIIPEIRHILTHA
                     SSGQR"
BASE COUNT         2442 a         3828 c         3949 g         2055 t
ORIGIN      
        1 tagccacgat catgccgtca cccgcctctt tgcgtactcg tcccagtgcg ccgcaagaaa
       61 gccccgtatc ccccgaccgg gccgataagc cgggtatatc cggcgataag ccgcctcggc
      121 cgctgcaagg ctctccaccg gcaggacgtc ggtgtgggcc gcaaggtaga ggagcccgat
      181 cgccgccgac cgggagatac cgaaaccgca atggaccaga accggccggc ccttcgcgag
      241 acaccggtcg atgaagagca gggcggcgtc gatcgcctct ttcgggacgt cgtcggggtt
      301 gtgcgtatcg accaggttga gcatcagccg gttgcctcgc cgggcgacca ggtgttcggg
      361 gtgatcctcc ggcaccgtcc cgacgggcga gcaggtgacg gccaggcagt ggtaagggct
      421 ccggcaggca tgcaccacgc accaggtctc gtgggcctcc accgtgacct cgtagtcctc
      481 ctgggtcccg acgtacaggt tttgatagat ctcgatcatc ggcgtacctc aagggcatac
      541 gccgcggccc cgaggagcgg cgagcgcccc ccaagttcgg agacggcaag ccgcgggagg
      601 gcgaggtagc ggtcgatata aggctccata tgccggataa cgatatcccc gtggtagcgg
      661 gcgagcggtc cgtcgaagac gatgatatcg gggttgtagg caacgattac ggccgagacg
      721 ccccgggcgt tcacctgccc gagcgcctcc atgaaggcga gggcgaccgg atcctctttt
      781 cggaccgcct cgaggatcgc ccgggtcgaa ccggcatcaa aggcgacgtc ccggacatcg
      841 gcctcctttc gccacgctgc aaagaacccg gggaggttct ttgcggaggc gtaggcctcc
      901 cagtggccct tatagccgca cccgcaggtg aggttgtaac gggtatcgac gggtatgtgc
      961 ccgatctctc cggcgttccc cgtcgccccg aggaggagac ggccgttcac taccgcgccg
     1021 ccgcctatcc cggtggaaag ggtgacgtag acaacgttat cggagccgcg ggcagcgcct
     1081 gcccaccatt cgccgaggac gcccgcccgg gcatcgttga tcagggcgac cggtagatcg
     1141 aaccgttcgc tgagcggttc gacgatctcc acgaccggga aggcgatgtt tggtgagttt
     1201 acgacccacc cgcggtcgag gtccagggga ccggccgacg cgacgccgat cgcggtggcg
     1261 ttctctcccg gcgccgacgc gaggagggtc tcgatccggt cgatgatcgc ggcggtgatc
     1321 gcatctccgg aaggccccga ggccggcgtc ggtgcggcgg tgtgggccag tatggtcgca
     1381 tcggcggagt cgatcagggc ggcgcggagg ttggtcccgc cgaggtcaac ggcaatcacg
     1441 gtcgtcatgg gttctccgga gagcagatgc ttctcttcac gaggttacgc gagcccgttg
     1501 cataaacctg cccccgcgcc cggatcagat accctccccc accgccatgc aggggtactt
     1561 ctcccgggga tacctgccct ccagggactc gcggacgaac tgctcccggg ccggagccgc
     1621 aagaaggctc tcgtagacct cttttctgac gccggcgtag cggtaagcgt caccgctctc
     1681 acatatgacg aggagcgcct tcgtcgtggg gtcgtaaccg atggatcgta tgccgccggc
     1741 ggaagacccc gctgatggtt gagacatgcg agctggtgtg gtcggcagcg atgatagcgg
     1801 ttgcggccgg tgatcgtgcc ggtcagcgga ccttctcgta ggggtaacgc agccggatga
     1861 aatccccgaa gtaccggcct ttcgaccggg ctgcaagcaa tccctcgtag acctcgggcg
     1921 gcaccccgac gtagttgtag accccgccgc tgtgaaactc gacctccagg atctcgtctt
     1981 cttcgttata accgaccgat cgtatgttgg tggactcgac ggcctggcgt tgcatggacg
     2041 ggggtggagg cggtgatggg gtataagggt tgtgggaggt gaggaggaat gcccggggag
     2101 tgcgggttac ggcatgacag agaaggcata gatgtagtcc gcaccggtcc cttcccggta
     2161 gcctgtcagg ggatcatggt cggcgtcgac aaagatcgca accgccgtaa ctccccacgg
     2221 gttcgcgggc aagatctgtc cggacgtaga ggatgccctc gcggatatag caggagagag
     2281 cggtgatatc ggggtcgagg aagtcccccg ccgggtcggc cacgccggcc gcagaggcaa
     2341 tagcgaccag caggagcgcc ggcagcacaa agagcacgca tccccacccc cggtctgccg
     2401 gcatcatcgg tcccgggtgt gcaggtgccg gagggggata aagatgccag atgcaggtta
     2461 tccggcggtt cttccggata ggctttccgg ggcaggaaag ctgctggagg atgctctatg
     2521 tggattctat agagcaatct ctcttatcga ggcaatcggg tctggcatac cggggtgagg
     2581 gctgcattcg ccgggccaaa acccgggact tccctgacag ggggagggag cgatcaccgt
     2641 atctctcttt gatcacttct aaaaattgtt ccgcatcgtc gataacgacc agaacctctt
     2701 ctgctgagag aatccgtgag atcgaataat caacctcctc ccgaagttct tccgcgatgc
     2761 gaaatgccct gccgaactcc ggatcgatca ccccggtccg gataaactcc gaaccgaatg
     2821 tagcgatcag gcccctgtgt gtccggactg taattccgcg ggtgagcagc agcgctgttg
     2881 ccgcaaaaga catggaataa tacgcgcgat tcacgggatc ttcgcaaaat ccctcgttca
     2941 gaagatatcg ggcggcagca agccgctgct ctgctctccg gagtaattcg tgaacagtcg
     3001 cgcccttatc caacccgggc accctcggcc ggtaccgcct tcaaaaacgg cgaatttcgc
     3061 ctcctctcca gatcgccgcg agagagcacc ttgaccgaga ggtttacgcc tgtctcgagc
     3121 atgatgtcat atgcaagccc gatcaggctc cgcctgaggc ggaagtcttc ctcgttcgta
     3181 atcaccagca ggtcgagatc cgatgaaggc gttgcctcac cgcgtgccgt tgagccgaag
     3241 aggatcacct cctcgatgcg gtcaccccac cggtagatgg ccttcgcaag gaatgcatca
     3301 agtgcggtgc ggaacgaggg atgcatgggt atcaatcctg tatcgcatct tccgggtgat
     3361 atatcttcgc gtggaagggc cggtgacccg tgcatgatca ctactcaacc cggggaagga
     3421 aggcgatggg cgggcgcctc ggcatcacct ggaaagagtt cgatctcgaa cagttccggc
     3481 gcggcatggt cgttgaactc gagcacggcc tccacgaccc ggtgacaaac gtcaccgacg
     3541 acgacctctt cctgacggcg aagacagcgc ttgcgcacct caacgaattc cccgacaact
     3601 acgaccggct cgaggagcag gcggaggcct accgggcgga acggagagcg gcatagcgat
     3661 tgagcgttcg ctgtcgcccc tgtagtgccg ggcggtcgat gcgcaacaac tatcgcccct
     3721 ccgcgccaat catggcatat ggaacggcgt gggagagcag aatggatcaa gatacggcag
     3781 cggcgggtca tcgacgagaa agagtactac gccgaccggc tctacgagac ccggcgtgac
     3841 gcaacctcct acggccgcat gctgcagaac caggataaag acgtcctcat cctcgggacc
     3901 atccacgaag gcgggcaccg gggctacgcc gtcttcgtca ggaggtaagg cagccggctc
     3961 aacctgcccg gcactcccgg atcgccggcc ccgtgcatcg ttcccgccac tcgccatgga
     4021 gaacgccgca gacgtcgata tcctcaacga ccccccactt cctgacgtgc ccccggaggt
     4081 gcgcttcgtg caccataccg cacttctcca tcacccggcc cgaggcgcgg ttccgggaga
     4141 agtgcatggc atagacccgg tgcagcccga ggaccgagaa cccgtactcg atcaccgccc
     4201 gggtggcttc cgtggcatac ccccgccccc agtacggcct ccccatccag taaccgagct
     4261 ccgcccggcg gtgatcgcgg tcgatctcca cgagcccggc cgcgcccacg agatctccat
     4321 cgcaggtgac ggcgtagatg gcatgcaccc cccgctcaaa ccctgggcgg tgcgtcgcaa
     4381 tccaggcctc cgccaccccg tcggggtagg ggtaggggat ctcgagggct gaggacgcga
     4441 ccgcatactc gccggcgagg cgctgcacca tggaagcatc cgcaagggtg aacggccgca
     4501 ggaggagccg gtcggttacg aggatgggct gtccggtcac gggaaggcgc ctccctcccg
     4561 gagaatcccc cagatatcga catcctcaaa gactccccac ttcctgatat gctcgcggaa
     4621 gtggccttca tgcaccattc cgcacttctc catcacccgg cccgaggcgg ggttccggga
     4681 gaagtgcatg gcgtagaccc ggtgcagccc gagcgccgag aacccatact cgatcatcgc
     4741 ccggaccgcc tcggtggcgt accctctgcc ccagcaggac cgtgcgaccc agaacccgag
     4801 ctctgcccgg cggtggccgc agtcgatccg caggcggacg ccgccgatca gccttccttc
     4861 cccggcaagc gtgatggcga aggctgcggt atcaccgcgc tcgatacctt cgcggaggga
     4921 ggcgatccag agctcggcaa ggccatcagg ataggggtga gggaggaggg ttgtcgcggc
     4981 aacggcgtag tcgccggcga gctgctgcac cgtgaaagcg tccgcaaggg cgaacgaccg
     5041 caagagcagg cgatcggtcg cgaggatggg ctggttctcc atatgcatgc tcattgcacc
     5101 ggccggatat aagccatgcc gcgccgccac ccggaccgca aggagagggt gatccagaga
     5161 ccacggcaac cgccacccac ccggccgtat aaccccccga taaccacacc cggttatacc
     5221 ctggtgcccc atcttccggg attatgtggc ccattagcga ttgggagcca ccctgccctt
     5281 ccgtttggcc ggaagttgta tgataaccgc cctatctgca aatcagcaac ctttattaat
     5341 ttttgttctg tattccatca tacatgatca aagtcgcaat caacggttac ggcaccatcg
     5401 gtaagcgggt tgccgatgcg gtcgccaaac agcccgacat ggaagtggta ggggtctcaa
     5461 agacaagcgt ctcggcagaa gcgttcgtcg caaaaaagcg tggatatccc ctctacatcg
     5521 ccgatatcgg aaagaaagca gcgttcgaga aggccggcct tgatgtcgcc ggcgacgtcg
     5581 aggcgatgct gaaagccgcc gatatcgtcg tggatgccac ccccggcggt gtcggagaga
     5641 agaaccgccc ggtctacgaa cgtctcggga agaaggcaat ctatcagggc ggcgaggagc
     5701 acgaggtcgc cgggttctcc ttcaacgctc atgtgaacta caaggaggcc gaagggcacc
     5761 agtttgcccg gatcgtctcg tgcaacagca ccgggctcgt ccggatcatc tctgccatgg
     5821 accaggcctt cggcgtcgac cgggtcagag cggtgatggt ccgccgggca tcagaccccg
     5881 gcgacgtcaa gcgggggcct gtggatgcca ccgtccttaa cccggccacc ataccgagcc
     5941 accacggccc cgacgtcaac accgtcctcc ccgacgtcaa catcgtcact cttgcgatga
     6001 tcgtcccgac gaccttcatg cacatgcatg ctatccagat ggacttgaag aaggagacca
     6061 ctcgcgaaga ggtgctgaag gtctttgaga accacagccg catcgggctc gtccgcaagg
     6121 ccaccggtat caagagcaac gcacagctcc gggagtacgc ccaggacctc ggccggccaa
     6181 gggccgacgt ctgggagaac ggcgtcttcg aggagtcggt atcggtcctc ggcgggaagg
     6241 agttctactg tttccaggcc atccaccagg aggccgacgt catccccgag aacatcgact
     6301 gcatccgcgc cttgatggga accgtgaagg atccggagga atccatccgg atgaccaaca
     6361 aagcgctcgg gttcgtcgcc atcaggtgaa cggcagggcc agggggaaga accattaact
     6421 ccccggcaga cccactcttt tcaatggacc catacgaact cgcgaccaga aataccgtag
     6481 aggtcgtcac cgacgaggaa ctgcgtgcgc tcatcgaccg cccggtcagg cgggtctaca
     6541 ccggctacga gccgagcggg gagatccatc tcggccatat ggtgacggtg aacaagctga
     6601 tggacctgca gaaagcaggg tttgaggtga ccgttctcat cgcggacctt catgcgttct
     6661 taaaccgcaa aggcacgatg gacgagattc gcgagatcgc gaagtacaac cgtcgctgtt
     6721 tcgaggggct cgggctggtc ggcgcgaagt atgttctcgg gtcggatgtg cagctcacgc
     6781 cggagtacga gcttgccgtc ctcgaacttt cccaggcgat caccctcaac cgggcgaaga
     6841 ggagcatgga cgaggtcggg cggcagatgg ataacccgac ggtctcgcag atggtctatc
     6901 ccatcatgca gatggtcgat atagcgacct tgaaggtgga cgctgccgtg ggcgggatcg
     6961 atcagcgaaa gatacacatg ctcgcgcggg aacacctccc ctcgatcggc tacccggcac
     7021 cggtctgcat ccacacgccg atcatcaacg gccttgacgg gaagaagatg tcgtcgtcgg
     7081 ccggcaacgt catatcggtc gccgacgccg cggaagatat ccggcagaag atgaagaagg
     7141 cgttctgccc gccggaggtt gagaacaacc cggtgctcga gatcctgcgc taccacatct
     7201 tcccccgggc cgggacggtc accatccgcc ggcccgcgaa gttcggcgga gaccgggagt
     7261 ttgccgccta caatgacctc gagcgggcct acgccgccgg cgagatccat cccctggacc
     7321 tgaagaacgc ggctgccaac catctcatcg atatcctcgc ccccgtacac gactacgtct
     7381 gcagcaggtg atccggccgt gggccatgat gatatgcacg agcggttcga ccgcgagatc
     7441 gaggcgatgg gtgtccggat aaaggatgcc gatgcactga aggtacggga cgacgtcttt
     7501 gacgaggtca ctctcgtcgc gctctacaaa cttgtccaca aaaaactgat ctcggtcatc
     7561 ggcggcccta tcagcaccgg aaaagaggcg aacgtctttt atggcgagcg cgacaaaagg
     7621 gggatcgcga tcaagatcta ccgcatccag acggcgaact tcaaggcaat gaccgagtac
     7681 cttgcaggcg accgccggtt tgcgagcatc cgggggtcgc gcaaaggcat cgtctttgcc
     7741 tggacaaaga aagagtacag caacctcgcc cgggcgcacg aggtggggat cccggtcccc
     7801 gaacccctcg cgtttgaccg gaacatcctc ttgatggagt ttctcgggcg cgaagaggta
     7861 ccgtacccgc agctccggaa cgccgaagtt gcggattacg gggtcacgta cggcgagatc
     7921 ctcggctata tcgaacggct ttatcaggag gcacgcctgg tccatgccga cctctccgaa
     7981 tacaatatcc tctactacga gaaaccatac gtgatcgata tgggacaggc ggtcaccctg
     8041 gatcacccac gggcgtccgc gttcctgatc cgggatatca ggaacatcaa ccggtatttc
     8101 tcccgttact gcgatgtgca ggacgaacag gagatcatcg gtgcggtcac cggcagcaga
     8161 ggccgggagc gataaccatc gggagacacg ataaccatga ccagacagga gctaaaagtt
     8221 acatccgcaa ggcttggtgt actcatcggc aagagcgggt ccacgaaacg cgagatcgaa
     8281 gagaagaccg ggacaatcct ccggatcgac agcgaggagg ggatggtgac gatcgaggcg
     8341 gaggatcccg tcgccgtcat gacgacgaca aacgtcgtct ccgcgatcaa ccgggggttc
     8401 tcgccggagc gtgcataccg gctccttgag gacgaagaca tgatgttcga cgtcatcgac
     8461 ctcgccgacc tctcgggcac gacccggcac ctcgagcggc tccggggccg gatcataggg
     8521 aaagcaggca catcccgcgc ccagatcgag gatatgaccg gcacggagct atcggttcac
     8581 ggaaagaccg tcgctatcat cggtctcccg gaccaggtcg agaccgcacg aaaggcggtg
     8641 gagatgctga tccagggcgt gccccacgag cacgtctacg ccttcctcga ccggaagaag
     8701 aaggaagcaa aacaggcgat gctggagtac tattcgtgat gcggccggag cacaccggcc
     8761 ctgaacaccc cgatgataac acgaagaaaa tacagtctgc agtggaaatg aaggggtata
     8821 catggagata gccggcctcc ccatcccgcc ggatcttctg gccgactaca cggggcgggg
     8881 gatcacggaa ctctacccgc cgcaggcagc ctgcgtcgaa gcgggtcttt ttgcggggaa
     8941 gaacctcctc atagccatac cgacggcaag cggcaagacc ctggtcgccg agatggcgat
     9001 gcaccaccag gtcggccggg gcggcaagtg cctctacatc gtccccttgc gagcgctcgc
     9061 gagcgagaag ttcgaggagt tctcgggaaa aggcctccgc gtcggcatcg ccaccggcga
     9121 cttcgatcga agggacgagt acctggggag aaacgacatc atcgtcgcga cgagcgagaa
     9181 ggtggactcg ctcctccgga accggacgcc gtggctcgcg gaggtcaccc tgctcgtcct
     9241 cgacgaggtc cacctcatcg acgacccctc ccggggggcg acgctcgaga tggtgatcgc
     9301 gaagctccgg cacaagaacc cggcgatcca gatcatcgcc ctctccgcca ccatcgggaa
     9361 ccccggggac ctcgccgggt ggctgggcgc ggagctcgtc gagagcgact ggcggccggt
     9421 ggatctccgg gagggagtct tcttctggaa cggcatccgc tttgccgacc gcgaccgcga
     9481 ggtcgaacgc cggagcaaat acgaggacct ggacctcgtc ctcgatacgg tcgcggaggg
     9541 ggggcagtgc ctggtcttcg tcagctcccg caagaacgcc gaggcgtttg caaagcgtgc
     9601 ggcatcgggg ttgaagctca aaaacccgat cctcgccggc tacgccgata agctacgctc
     9661 gagcgcctcg accgatatgg ggaagacact tgccgcctgc gtcgcgcagg gtgcggcgtt
     9721 ccaccacgcg ggccttgccc gcgaagagcg ccagatcgtc gaggcagggt tccgggaggg
     9781 gcagatcagg gtgatcgcct ccacaccgac gctcgcggcg ggcttgaacc tccccgcccg
     9841 gcgagtgatc gtccgcgact acctccggtt caacgccggg gaggggatgg tcccgatccc
     9901 cgtccgggag taccggcaga tggccgggcg ggccgggcga ccgcacctcg acccctacgg
     9961 cgaggcggtc ctgatcgcga agtccgagga gatggtcgac gagctcttcg actgctacat
    10021 caacgcgccg gccgaggacg tccggagccg gtgcgcggat gaggcggtcc tctgcaccca
    10081 catcctctcg ctcatcgcga cgggattcgc ccgcgaggcg gatgaggtgt tcgcgttcat
    10141 ggacgggacg ttctacgccc accaggggga gagcccccgt gcactctcgc gggcggtcca
    10201 tcgggcgctt gagttcctgc gcgaggcgga gatgatcacc gaggtcggtg agtggatcga
    10261 ggccacggag tacgggtcgc tcgtctcccg gctctacatc gatccccgga gcgccgaggt
    10321 gatcgtggcg gcgatgatcg accggcagga gtacacggat atcgggttcc tgcacctcct
    10381 ctgctcgaca cccgatatgc cgacgctcta catccggcgg gacgacatgt acctcctcga
    10441 ccggttcctc gccgaccacc gtgacgacct ctggacggat attccctggg atgcgggcga
    10501 ggggttcgac cggagcctca agaccgcgct cctccttgcc gactgggcgg acgaggtagg
    10561 cgaggatacg atctgcgagc gctaccgcgt cggcccgggc gacatatacg gtatggtgga
    10621 gagcgtcgcc tggctggtcc acgctgcccg gagtctcgcg aaactctttg ccccgcacct
    10681 tgcggcgccc atcgaggaga tggagctccg gaccaaacat ggcatcaaga aggaactcct
    10741 ccccctcatc aggcttcacg ggatcgggcg cgtccgggcg cgccggctct tcaacaacaa
    10801 cctcgggtcg atcgaggcgc tccgcgcggc cggaccggag aaggtcggaa agatcctcgg
    10861 ccaggggatc gccgcgcgga tattcgagca gcttgagggg acgcgggagg aggtcgggga
    10921 agtcagcgaa gagcagtcga ccctctcccg gttcggatga agagagatgg tagagatgac
    10981 ggagacggta aaaacaggag agatggtgga aacggcggaa acggtagagg cggcatgcga
    11041 catcttcccg gcggtcttcg cggtcgatga gaatatccgg tttctgcgcc ggatccgccg
    11101 gatcgccgac gagcaccgga cccatatcat actcttcgat gccgaccgcc tcgccgggcg
    11161 cgcccacgtg gaggcagctc tccggcacgc ctggcgatcc tgggccggcg gcaacccgat
    11221 agcaaactcg atcgagatgg aagcgctcct ttacgccgcg gggacccggc agtgtcaggt
    11281 ggctgcatcc tttggcatcc atcctggcct gaaccgctcc tacatcgccg tctgcccatc
    11341 ggcgccgggt atccgtgacc atctcgccgg cctcgtgacg ttcgtcgacg gggagcacga
    11401 tgagacgatc gatcctggaa aacgtgcgcg ccttgcggac ctcttcggca tcacgcccga
    11461 ggaggtcgcg gtggtgggcg aggaccggtt ccgcgacctg gtcatcgagc gggtggcgct
    11521 cctcgacgtc taccgctgac ccgacgaggc gtgggtgagg atgtgccgga tctcggggat
    11581 gatgatctcc cgcgcggcga gggtgacggc cttcgtcgag cccggtatgc agaagaccgc
    11641 ccggccgtcg atgacgcctg ctatcgcccg ggaaaggatc gcagcggtcc cgatctcctc
    11701 gtaacttttg aggcggaaga gttcgccgaa cccgtcgatc gtctttgcaa ggagcggcgc
    11761 gaccgcctcg atcgtcacgt cgtcgggggt gagcccggtg cctccggtga cgatgacaca
    11821 ggttgcacgc gaaatggccg cagagacctc ggcacggatc cgcgcgacat catcggggac
    11881 cacggcatag tgggtcacct cgatgccgtc ggcggagagg aggtcgatga gcgcctttcc
    11941 gctggcgtca gtcccgggtt tccggctcga cgagacggtt atgacggccc cggtcacggt
    12001 gaggggttta agatggttac tctccataag atcttcctgt acccctgtac cgcgtatttt
    12061 acttctttct atcctttgcg ggaggggggg cgatccccga cccctttgtt tcgactggaa
    12121 cttaaggggg gcacgcgagg ggagggggaa ggtcggtgtg gtggggagag aggagaggat
    12181 cacctccccg gcagatatcc gggttcatca agaagagttg acaggaggcg ggggcaggag
    12241 agagaaggtt atcccgggga tggctacagg agaa
//
LOCUS       DAOSEE010000063        11973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_2060_length_11973_cov_214.423729, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000063 DAOSEE010000000
VERSION     DAOSEE010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 11973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11973
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2060_length_11973_cov_214.423729"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="PLI76_06265"
     CDS             complement(<1..1003)
                     /locus_tag="PLI76_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOI61670.1"
                     /translation="MAAGEACPTCPRCRAGVCEIHGPIVSRRDIAVFTIAGILLLPGI
                     LIGAFTPYPLAGTVLLLAAAVISGYDVLRAGVLSFIRLRFSIAVLISIAAAGAFLTGN
                     PAEGATVLYLYAIAEFLEEYAAGRAHRSIASLLDIAPQTARVRRDGEEVTVGVGDVGV
                     GEIVIVKPGDTIALDGIVTAGASSVDQAAITGESVPVAKGIGAGVYAGTRNVDGYLEV
                     RVTKPEAESTIARVVALVAEAQAHTSPTEAFIERFSRYYTPAVIIVAAFLVVVPPLVF
                     GIPFLEAFYRALILLVISCPCALAISTPVSMVSGITTAAHNGVLIKGRDSLEAVGLAR
                     "
     gene            1257..3704
                     /locus_tag="PLI76_06270"
     CDS             1257..3704
                     /locus_tag="PLI76_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOI61671.1"
                     /translation="MPTQDSTTPPRDDPGATVPCRIWICGPGGEALHLPDPGWAGRIH
                     PEDMDAVFSGWKRSVTAGAPWSRRYRIQDNGGEYRTVVSSGIPVRDARGRVVLWVGVT
                     GRDKSCGEGRELATLAAIARVIEEGDADPGEVVRRVVRLLPRGFWHPEEVSVRVVPGA
                     AVPGPGKVVAGRLVVEFRDRPEGMGDPYLPQERDLIYAVAAMLEAAVAPAYGETGPSS
                     IAERRQAEEALREQQIALDNRVRELATLYTMTDIVERPGISVDEILAEVVAILPAGWL
                     HPGDAAARITLDGREYRSPGFVETCWRQESPIVVCGRIAGKVEICYLHEEPAKDEGPF
                     LLEERSLIDAVAARLGRIIERVRADEGLRRSEEKYRLLFEQMLESYTLYEVILDDAGD
                     PIDYRLAELNEKATAVFGCSRDELIGRRLFDIFPAIREGALAIYGEVAETGIPTRRRL
                     QEPKSGRWYDLHIYRPEIGRLVVTGQEITGQKKAELALQKSEERFRGIFEQAGTGIAL
                     IDPEGRIMEANPAFVRMFGYDEDELHAMLFLDLIYPPERETARALLQKTASGESARRE
                     KRYVTKDGRILRGRLTTSLLCDPEEEGLVIGMVEDVTDRREMQNALRESEERFREIAQ
                     RSFDMIYTCYNDRGITYISPAVTRILGYTPEEMIGVHCGEYILETSRQEWLEAESRIA
                     RGEPVAGLVVGFRRKNGTAAAVEMNESPIMEGGRVIGVQVVGRDISDRKRYEDMRLQA
                     FSQIEQNIEQFAVLADHIRLPLQVILGTADLTDDTNAAEKIREQVERINRIVKQLDEG
                     WVESREIREFLRRNELV"
     gene            3989..5914
                     /locus_tag="PLI76_06275"
     CDS             3989..5914
                     /locus_tag="PLI76_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE3 domain-containing protein"
                     /protein_id="HOI61672.1"
                     /translation="MQTNSRGTETATGYHPTDGTDIRQDHAKQGAASPGILTSLIVFT
                     VLLAALAATSLYNYLLFHTIAELVAVGIAIAVFTVAWNARTMRGNSYLLVAGTGLLFV
                     AGVGLVHTLAYEGMGIFSGYDANLPTQLWIASRYLLAGTLLAAPLLLRRNPAPWGVFA
                     AFTGVTGALLLSIFVYPVFPDCYVVGSGLTPFKIVSEYVIAALLALGAVAVYRLRDIF
                     SPRVAANLIAAILVLVASELAFTLYISVYGFANMVGHLLMVLSFGLFYVAFVETGIRQ
                     PYAVVFTGLARSEEQLRREKDRLEQYLDIAGALFVVLDRDGRITLVNRRASEILGYPA
                     NVVVGEPWIERFVPPEERERAHALFLGLMGGKSAPVYARTPVLTAGGGERIIEWHNTV
                     LRDDDGVACGTLSSGVDITERQHAEEALRQANVKLNLLSGITRHDILNQVTVARAYID
                     LTAEDSDEPGIGNYLARARTAVEEIQKQVEFTRDYQDMGVAAPVWQRPEDLINESASD
                     LALPAGVRVRTDLADLEIYADPMVKKVFYNLIDNAIRHGEKITEIRFSCAKSGRDLLL
                     LCEDDGTGIPDGEKETIFHEAYKRRHGHGLFLVTGILGITGMTIRETGIPGEGARFEI
                     RVPNGGYRGDNERCAAP"
     gene            complement(5990..6985)
                     /gene="ruvB"
                     /locus_tag="PLI76_06280"
     CDS             complement(5990..6985)
                     /gene="ruvB"
                     /locus_tag="PLI76_06280"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOI61673.1"
                     /translation="MKDRITSPVALREEPDDAAIRPARFDEFVGQREVKETLAIAIAA
                     AKRRGESLDHILFSGPPGLGKTTLARIIAREMEVAIRTTTGPVLDRPADLAAQLTALS
                     RGDVLFIDEIHRLNPVVEEILYPAMEDACIDVMIGEGPGARSVQLPLEEFTLVGATTK
                     VGLLGSPLRDRFGFIFRLNLYEVEDLATIVRRSAGILHTPITPEGALEIARRSRGTPR
                     IANRLLRRVRDFALVRGDGSIDEKTADLALTMLGIDRLGLDELDRRILSVIVHDFDGG
                     PVGVKTIAISIGEEVRTVEEVYEPYLIQIGFIKRTPQGRETTPAARDHIESASRG"
     gene            complement(6982..7572)
                     /gene="ruvA"
                     /locus_tag="PLI76_06285"
     CDS             complement(6982..7572)
                     /gene="ruvA"
                     /locus_tag="PLI76_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOI61674.1"
                     /translation="MIDHLSGELASTGDRWVVIDIGGIGYRVQVTGPTLQDLAGTGGR
                     VMVHTHMVVRDDDIQLYGFAHPRERELFTILIGVTGIGPQTAMNILSRVSFEDFAIAI
                     INDDEKVLTRIPGIGPKSAKRLILELKDKMKKCAATLPATSHRAEAGDAVSALVSLGF
                     SGREAEEAVDAALSALSAPTVQDLIRASLARLRERP"
     gene            complement(7575..8045)
                     /gene="ruvC"
                     /locus_tag="PLI76_06290"
     CDS             complement(7575..8045)
                     /gene="ruvC"
                     /locus_tag="PLI76_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOI61675.1"
                     /translation="MTVIGIDPGVARTGYGVLKRGDPYPVPLTFGCIETGSDSRPSRR
                     LLEIYERVSLLFDEYVPDSVVLEQLFFSKNITSAMHVSEARGVILLAAEERDISITEY
                     TPNQVKQAVTGSGRADKHQVQEMVRRLLRLQEIPRPDDAADGLAIALCHINMVR"
     gene            8322..8867
                     /locus_tag="PLI76_06295"
     CDS             8322..8867
                     /locus_tag="PLI76_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61676.1"
                     /translation="MPRRKKTVSSTPLLDSLSVDQARFVLARLIGDDPALAARAEGIA
                     RDVLRMVDTEGVAESVASDLSCIDIEEVWETSGRRRDGYVYPSERAWEMLDDALEPHK
                     GEMMAYLKRGMVEESRLCCAGILLGIRRFEQDSGSDLLEEAPDYCNETFDSVREEWEK
                     AVGDAAQVRLLARFIEEKGLS"
     gene            complement(8894..9811)
                     /locus_tag="PLI76_06300"
     CDS             complement(8894..9811)
                     /locus_tag="PLI76_06300"
                     /inference="COORDINATES: protein motif:HMM:NF024820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOI61677.1"
                     /translation="MGAKRLNRMAMRAATERSLIDATALIQGREFESIEEANAFLQKV
                     LKEGGLPPAPADTPLLQAQQVAYDAMEATGRRRVVLARRALEISEDCADAWVILAGEE
                     RKSLRKALEYARKGVAAGERALGKEVFEEEVGNFWTLIGTRPYMRALFALAELLWATG
                     DKDEAIGLYQEMLRLNPDDRQGVRYIVMQIFAEANRDDLLESILDEHGEDPTATVAYA
                     RALWLFRKEGAGPAADAALDDALQKNPFVPQYLLAFRALPSRMPDYYGLGDRNEAIVY
                     AMTALDGWIGTEGAPAWLASRVRKMRSSR"
     gene            complement(9912..10157)
                     /locus_tag="PLI76_06305"
     CDS             complement(9912..10157)
                     /locus_tag="PLI76_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HOI61678.1"
                     /translation="MPEGLTAWRAYLYVVSFIALIIVIAGSVNLIAVAIEVFAYPAPQ
                     PYPASQYYYSALPGSVAQVAVGFIVWAYHWRLLGRER"
     gene            complement(10162..11169)
                     /locus_tag="PLI76_06310"
     CDS             complement(10162..11169)
                     /locus_tag="PLI76_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61679.1"
                     /translation="MRRLFGAATVIVLLSLVLAAGCTSSRPPAGYVTIRSVDISIPDG
                     QPPSNVTTVVVVPYLDAVYADVGNVDLRISAKEAGTDIVVAQKVLSIGNLTNGRTEKA
                     EVTLALENGRRYDIWATVWQDGRMRESGSVSVSLPDRTTAGRRLAYSFLAVTAIDVMT
                     PDIGADPITLDIITGIKNSAAAASGDLAMDIRVVNLQTGITAVRESRPLGTVAGEGAD
                     EKQVRVTVPNGYDYEISIKLVEGGAAFATSTGRVTLAPPPQVILGNGAVLDSGKVTSP
                     VSLPVPTAAPTSPSSAQVGQFKIQSGGEPTPTQASGFTAAFAAAALLGAGAAVRAGRR
                     R"
     gene            11654..>11973
                     /locus_tag="PLI76_06315"
     CDS             11654..>11973
                     /locus_tag="PLI76_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOI61680.1"
                     /translation="MIRTEKLTKVYNGKTAVDGLDLTVGEGEVFGFLGPNGAGKSTTI
                     LMLTGMIEPTGGRCLVDGIEVAKDPLAVKKIIGYLPEDVGFYGNMTGEQNLDYFARFY
                     GMNAR"
BASE COUNT         2207 a         3797 c         3871 g         2098 t
ORIGIN      
        1 cccgggcaag ccccacggcc tcaagggagt cccggccctt tatgagcacg ccgttgtgcg
       61 ccgcggtcgt gatccccgag accatcgaga ccggggtgga tatggcgagc gcacaggggc
      121 aggatatgac gaggaggatc agcgcgcggt agaacgcctc aaggaacggt atgccgaaga
      181 cgagcggcgg aaccacgacg aggaaggcgg cgactatgat gaccgccggc gtgtagtagc
      241 gggagaaccg ctcgatgaac gcttcggtgg gggaggtgtg ggcctgggcc tccgcgacca
      301 gcgccacgac gcgggcaatc gtgctctccg cctcgggttt tgtcacccgg acctcgaggt
      361 aaccatcgac gtttcgggtc ccggcgtaga cgccggcccc gatacccttt gccaccggga
      421 cgctctcccc ggtgatcgcc gcctggtcga ccgaggatgc gccggccgtg acgataccgt
      481 cgagcgcgat ggtatcgccc ggcttcacga tcacgatctc ccccacgccg acatcgccga
      541 cgccgacggt cacctcttcg ccgtcccgcc ggacccgggc cgtctgcggc gcgatatcga
      601 ggagcgatgc tatcgagcga tgcgccctcc cggccgcgta ctcttccagg aactcggcga
      661 tggcatagag gtagaggacg gttgcgccct ctgcggggtt tccggtcagg aacgcacccg
      721 ccgccgcgat ggatatgagc acggcgatgc tgaaccggag ccggatgaag gagaggactc
      781 cggccctcag gacgtcgtag ccggatatga ccgcagcggc gagcagcaga acagtcccgg
      841 cgaggggata cggcgtgaag gcgccgatga gtattcccgg gagaaggagg atacctgcga
      901 tcgtgaagac cgcgatatcc cggcggctga cgatgggccc gtggatctcg cagaccccgg
      961 cccggcagcg gggacaggtc gggcacgcct caccggcggc catggtccgc ctccgtctcg
     1021 ccggcgatgg ggcggcaacc cccggcctca accccgggga catgggtctc tctcatgact
     1081 gtacggaatc ttggtcatat cgaacttaaa aggtgctatc ggtggcgagg ttgaccgtgc
     1141 acaaacgtta taatgggttc aaaccaactg atcggggagc gatttcgtgc agcgaacccc
     1201 ggggttccag ctgaaagcga ctgccaatcc tgtttctcct cctgcaggag cagacgatgc
     1261 ctacacaaga ttccaccaca cctccccggg atgatccggg agccactgtc ccctgccgta
     1321 tctggatctg tggacccggc ggcgaggcgc tccacctccc ggaccccgga tgggcgggca
     1381 ggattcaccc ggaagatatg gacgcggtgt tttccgggtg gaagcgttcc gttactgccg
     1441 gcgcgccgtg gagccgcagg tatcgtatcc aggacaacgg cggagagtac cgcaccgttg
     1501 tgagcagcgg tatcccggtc agggacgcca gaggtcgggt cgtcctctgg gtgggggtca
     1561 ccggccggga caaatcttgc ggagagggga gggaacttgc gaccctcgcc gcaatcgccc
     1621 gggtgatcga ggagggcgat gccgatcccg gggaggtcgt ccggcgggtg gtgcgcctcc
     1681 ttccccgggg gttttggcac ccggaagagg tctcggtacg ggtcgtgccc ggggccgccg
     1741 tcccgggccc ggggaaggtc gttgccgggc gtcttgtggt cgagttccgg gaccgaccgg
     1801 agggaatggg agatccatac ctcccgcagg agcgtgacct catctacgcc gtcgccgcga
     1861 tgcttgaggc cgccgtcgcg ccggcgtatg gggagacggg gccctcaagc atcgcagaac
     1921 ggcggcaggc agaagaggcg ctcagggagc agcagatcgc tctcgacaac cgggtgaggg
     1981 aacttgcgac tctctatacc atgaccgaca tcgtcgagcg gcccggtata tcggtcgacg
     2041 agatcctcgc ggaggtcgtc gccatcctcc ccgccggctg gctccacccc ggggatgccg
     2101 ccgcgcggat cacgctcgac ggccgggagt atcgctctcc cgggttcgtg gagacctgct
     2161 ggaggcagga gagcccgatc gtcgtatgcg gccggatcgc gggaaaagtg gagatctgct
     2221 atcttcacga agaaccggcg aaggacgagg gaccgttcct tctcgaagaa cgctccctga
     2281 tcgatgccgt cgcggcaagg ctcgggcgga tcatcgagcg ggtgcgggcc gacgagggcc
     2341 ttagaaggag cgaggagaag taccgcctcc tctttgagca gatgctcgag agttacacgc
     2401 tctacgaagt gatcctggac gacgcaggag atcccatcga ttaccgcctc gccgaactga
     2461 acgagaaagc caccgccgtc ttcggatgta gccgcgacga actgatcggg cgacggctct
     2521 tcgacatatt cccggcgatc cgcgaggggg cccttgccat ctacggggag gtcgcggaga
     2581 cgggcatccc gacccggcgg cggttgcagg aaccgaaatc cggacgctgg tacgacctgc
     2641 acatctaccg cccggagatc gggaggctcg tcgttacggg acaggagatc accggacaga
     2701 agaaggccga gctcgccctc cagaagagcg aggagagatt ccgggggata ttcgaacagg
     2761 cgggaaccgg gatcgcgctc atcgatcccg aggggaggat catggaggca aacccggcgt
     2821 ttgtgcgtat gttcggctat gacgaggacg aacttcacgc catgctcttt ttagacctga
     2881 tctatccgcc cgagagggag acggccaggg cccttctgca gaagacggcc tccggggaga
     2941 gcgcccgccg ggagaagcgt tacgtgacaa aagacggcag gatcctgcgg ggccgcctga
     3001 ccacgtcgct gctctgcgat ccggaagagg aggggctcgt catcgggatg gtggaggatg
     3061 tcaccgaccg gagggagatg cagaacgcac tccgggagag cgaggagcgg ttccgggaga
     3121 tcgcccagcg cagtttcgat atgatctaca cctgctacaa cgaccggggg atcacctaca
     3181 tatccccggc tgtcacgagg atcctcggtt ataccccgga agagatgatc ggggtgcatt
     3241 gcggcgagta catccttgaa acgagccgcc aggaatggct ggaggccgag tcccggattg
     3301 cccggggaga gcctgtcgcg gggctggtag tgggattccg ccggaaaaac gggacggccg
     3361 ccgccgtcga gatgaacgaa tccccgatca tggagggagg gagggtgatc ggcgtccagg
     3421 tcgtcggccg ggacatctcg gaccggaagc gctacgaaga catgcggctg caggcattct
     3481 cccagatcga gcagaacatc gagcagtttg cagtcctcgc ggaccatatc cgcctgcccc
     3541 tgcaggtgat cctcggcacg gcagacctga ccgatgacac aaatgcggcg gagaagatcc
     3601 gcgaacaggt ggagcggatc aacaggatcg tgaaacagct tgacgagggc tgggtcgagt
     3661 cccgcgagat ccgggagttc ctgcggagaa acgaactggt ataacggcca ccatctcccg
     3721 gtcgcgggat ctgccggagc ggatcgtacc ccggcggctt caggcagcac gttctttcgt
     3781 cgaacgatat cccgtactcc tgcaccgggc agcagaacag ttatcggtca attgaatata
     3841 ctgtactgca gatacgggta gggttaacgt gccggccgtt gccgcgcccg cataaccatg
     3901 cgaccgtgat gagatccgaa aaaagagcac ctgaagagcg gttcagcgcc ccccacacag
     3961 ccggaagaac ccgacctgaa ctcgaaccat gcagaccaat tccagaggaa cagagaccgc
     4021 aaccgggtat caccccacag acggcacgga tatccggcag gatcatgcaa aacagggtgc
     4081 agcgagcccc ggcatactga cgtcgctcat cgtctttacg gtgcttctcg ccgccctcgc
     4141 agcgaccagc ctctacaact acctcctctt ccacacgatc gccgaacttg tcgccgtcgg
     4201 aatagcgata gcggtcttca ccgtcgcctg gaacgcccgg acgatgcggg ggaacagtta
     4261 cctgctcgtc gccggcaccg gtctcctctt cgttgccgga gttgggctgg tccacaccct
     4321 tgcctatgag gggatgggaa tcttttccgg ctatgatgca aacctcccga cgcagctctg
     4381 gatcgcctcc cgctacctcc ttgccgggac actcctcgcc gcgccgctcc tgctccggag
     4441 gaacccggcc ccctgggggg tgtttgccgc gtttacggga gtcaccgggg cccttctcct
     4501 ctcgatcttc gtgtatccgg tcttccccga ctgctacgtc gtgggctcgg ggttgacgcc
     4561 cttcaagatc gtgagcgagt atgtcatcgc ggccctgctc gccctcggcg ccgtcgccgt
     4621 ctaccggttg cgggacatct tctcgccccg ggtggcggcc aaccttatcg cggcgatcct
     4681 cgtgctggtc gcatcggaac ttgccttcac gttatacatc agcgtctacg ggttcgcgaa
     4741 tatggtcggc cacctcctta tggtgctctc gttcgggctc ttttacgtag cattcgtcga
     4801 gaccggcatc cggcaaccct acgcggtggt cttcaccggc ctggcgagaa gcgaggagca
     4861 gctccggcga gagaaagacc gcctcgagca atacctggat atcgccggcg ccctcttcgt
     4921 cgtcctcgac cgggatggcc ggatcaccct cgtcaaccgc agggcgtccg agattctcgg
     4981 ttatccggcg aatgtagtcg tgggcgagcc ctggatcgag cggttcgtcc ctccggaaga
     5041 gcgggagcgt gcccacgcgc tctttctcgg gctgatgggg ggaaaatccg cgcccgtata
     5101 cgcgaggacg ccggtcctca ccgcaggcgg cggcgagcgg atcatcgagt ggcacaacac
     5161 cgtgctccgg gacgatgacg gcgtcgcctg cgggacgctc agttccgggg tggatatcac
     5221 cgagcggcag cacgctgagg aggcgctccg acaggcaaac gtgaagttga acctcctctc
     5281 cgggatcacc cgccacgaca tactcaacca ggtcaccgtc gcacgcgcct acatcgatct
     5341 caccgcggaa gatagcgatg agcccggtat cgggaactac ctcgcgcgtg cccggaccgc
     5401 agtggaggag atccagaaac aggtcgagtt cacccgggat taccaggata tgggcgtcgc
     5461 ggcgcccgtc tggcagagac cggaggatct catcaacgag agcgcttcag acctcgccct
     5521 cccggcggga gttcgggtcc ggacggacct tgcggatctt gagatctacg cggacccgat
     5581 ggtgaagaag gtcttttaca acctcatcga caacgccatt cgccatggtg agaagataac
     5641 ggagatccgg ttctcctgcg cgaaatccgg acgggatctg ctgctcctct gcgaggacga
     5701 cggcaccggc atcccggacg gcgagaaaga gacaatattt cacgaggcgt ataagcggcg
     5761 gcacgggcac gggctcttcc tggtcacggg aatcctcggt atcaccggta tgacaatccg
     5821 ggagacgggg atccccggtg aaggggcgcg ttttgagata agggtgccga acggcgggta
     5881 ccggggtgat aacgagagat gcgcggcccc gtagaccggc gggcaaccgg tcgatcggta
     5941 tcggttcaag aaggggatca ggcccgccgg agatgggggc gaagggccgt cacccgcggg
     6001 acgccgactc gatatggtcc cgtgcagccg gcgtcgtctc ccggccctgg ggtgtccgtt
     6061 ttatgaaccc gatctggatc aggtagggct cgtagacctc ctcgaccgtc cgcacctctt
     6121 ctccgatcga tatcgcgatc gtcttgacgc ccaccggccc gccgtcgaaa tcgtgtacga
     6181 tcaccgagag gatacgccgg tcgagctcgt ccaggcccaa ccggtcgatg ccgagcatgg
     6241 taagggcaag gtcggcggtc ttctcatcga tgctcccgtc gcccctgacc agggcaaagt
     6301 cacgcacccg ccggagcagc cggtttgcta tcctgggcgt tccgcggctt ctcctcgcta
     6361 tctcgagcgc gccttccggg gtgatcgggg tatgcaggat accggcgctc cggcggacga
     6421 tcgtcgccag gtcctcgacc tcgtagaggt tgagccggaa tatgaaacca aaccggtccc
     6481 tgagcgggga tccgagcaga ccgaccttcg tcgtcgcgcc gacgagggtg aactcctcga
     6541 gcgggagctg cacggaccgc gcccccggcc cctccccgat catcacgtcg atgcaggcgt
     6601 cctccatcgc cgggtacagg atctcctcga ccaccggatt caagcggtgg atctcgtcga
     6661 tgaagaggac gtcgccccgg gagagcgccg tcagctgggc ggcaaggtcg gccggccggt
     6721 cgagcaccgg gccggtggtg gtccggatcg ccacctccat ctcccgggcg atgatacggg
     6781 caagcgtcgt ctttccaaga cccggcggcc cggagaagag gatatggtcc agggattcgc
     6841 cgcgcctctt tgcggctgct atggcgatgg caagggtctc ttttacctcg cgctggccca
     6901 cgaactcatc gaaccgcgcc ggcctgatag cggcgtcgtc cggctcctcc cgcagggcga
     6961 cgggcgacgt gatccggtcc ttcatggtcg ctcccgcagg cgggcaaggg aggcgcggat
     7021 cagatcctgc accgttggag cggagagggc ggagagggcg gcgtcgaccg cctcctccgc
     7081 ttcgcgcccg gagaacccga gcgataccag ggcgctcacc gcatcgccgg cctccgcccg
     7141 atgggaggtg gcgggcagcg tcgccgcgca cttcttcatc ttgtctttca gctccaggat
     7201 caggcgtttg gcgctctttg gcccgatgcc cggtatccgg gtgaggacct tctcgtcgtc
     7261 gtttattatc gctatcgcga agtcttcaaa cgagacccgc gagagtatgt tcatggcggt
     7321 ctgggggccg atcccggtga cgccgataag gatcgtgaag agttccctct cgcgcgggtg
     7381 ggcaaatccg tagagctgga tgtcgtcgtc acggaccacc atatgggtgt gcaccatgac
     7441 gcggccccca gttcctgcga ggtcctgcag cgtgggcccg gtgacctgca cccggtagcc
     7501 gatgccgccg atatcgatca ccacccaccg gtctccggtg gatgccagtt ctccggaaag
     7561 atgatctatc atggttaccg caccatattg atgtggcaga gggcgatggc gaggccatcc
     7621 gcggcatcgt ccggccgggg aatctcctgc aggcggagaa gccgccgcac catctcctgc
     7681 acctggtgtt tgtcggcacg ccccgacccg gtcaccgcct gcttgacctg gttcggcgtg
     7741 tactcggtga tcgagatatc tctttcttcc gcagcaagga ggatgacgcc gcgcgcttcg
     7801 ctgacgtgca tggcagaggt gatgttcttt gagaagaaga gctgctcaag gacgacagag
     7861 tctggtacgt actcgtcaaa gagcagcgag accctctcgt agatctccag gagccgccgg
     7921 gatgggcggc tatcgcttcc ggtctcgatg cagccgaatg tcaggggaac cgggtagggg
     7981 tcgcctctct tgaggacgcc gtagccggtc ctcgccaccc cggggtcaat cccgatcacg
     8041 gtcatctccc tcacgcatga gtactccgtt ctcctgctag atacctattt cgcggggttc
     8101 cggctgccgg gcaccggttg acccggggtg ccgtcgtcgg gaacgctgcg cccttccatc
     8161 cctgcaggct gcggaaagca tggccgggaa gaggcgcagc cggcctgcag ggatgaaggt
     8221 tcgatcccgg agaagccccg ggccgggctc tctaccggag ccgtatcacc ctctgcgacc
     8281 ataacgataa ccctatctcc tccggcacac ctaccatcag catgccgaga cgaaagaaaa
     8341 ctgtctcctc cacccccctc ctcgactccc tcagcgtcga tcaggcgcgg ttcgtccttg
     8401 cacggctcat cggcgatgac cccgctctcg cggccagggc ggaaggtatc gcacgcgacg
     8461 tcctccggat ggtcgatacc gaaggtgttg ccgaaagcgt cgcaagcgac ctctcctgca
     8521 tcgatataga agaggtctgg gaaacctcgg gcaggaggcg cgacggctac gtctacccat
     8581 ccgaacgggc gtgggagatg ctggacgatg cgcttgaacc gcacaagggc gagatgatgg
     8641 catacctcaa gcggggtatg gttgaagagt cacgcctctg ctgcgcgggc atcctcctcg
     8701 ggatccggcg gtttgaacag gattcagggt cagacctcct cgaagaagcg cccgactact
     8761 gcaacgagac cttcgactcc gtccgggaag agtgggagaa ggccgtcggc gatgccgcgc
     8821 aggtgcgcct gcttgcccgt ttcatcgagg agaaagggct ctcgtgaaaa cacgcccgag
     8881 ccttgcagga aggttacctg ctgctccgca tctttcgcac gcgcgacgcg agccacgccg
     8941 gcgcaccctc cgttccgatc catccgtcga gtgcagtcat ggcgtatacg atcgcctcgt
     9001 tccggtcgcc gaggccgtaa tagtcgggca tccgcgacgg caacgcccgg aacgcaagca
     9061 gatactgcgg gacaaacggg ttcttctgca gtgcatcatc gagtgccgca tcagctgcag
     9121 gtccggcccc ctccttccgg aacagccaga gcgctcgtgc gtacgcgact gttgccgtgg
     9181 gatcttctcc gtgctcatcc aggatcgact caagcaggtc gtcacggttc gcctccgcaa
     9241 agatctgcat gacgatgtac cggaccccct ggcgatcatc ggggttgagc cgcaacatct
     9301 cctggtagag tccgattgcc tcgtctttat ccccggtagc ccagaggagt tctgcaagtg
     9361 caaacagggc cctcatgtag ggacgtgtcc cgatcagcgt ccagaagttc ccgacctctt
     9421 cttcaaagac ctccttcccg agtgcccgtt ccccggcagc cacgcccttc cgggcatact
     9481 ccagcgcttt tcgcaggctt tttctctcct ctccggccag gatcacccat gcgtcggcac
     9541 agtcctcgga tatctcaagt gcgcgcctgg ccagcaccac ccggcggcgc ccggtagcct
     9601 ccattgcatc gtacgcgacc tgctgcgcct gcagaagagg agtatccgcg ggggcaggcg
     9661 gcagaccacc ttccttcagc accttctgga ggaaggcgtt cgcctcctcg atcgattcaa
     9721 attccctgcc ctgtatcagc gcagtcgcgt cgatcaacga tctctcggtc gccgcccgca
     9781 tcgccatacg gttcagtcgc tttgctccca tggtcagtct atctgttcat tgatccctga
     9841 tgccggatag aggttccgca tggagtacgc acgagcatgg atgcaggcgg cgcccgggca
     9901 ggatactgcc ttcagcgttc cctcccaagc agcctccagt gatatgccca gacgatgaac
     9961 cccaccgcga cctgtgccac gctcccgggc agagcggagt agtaatactg cgatgccggg
    10021 tagggctggg gcgccgggta ggcgaagacc tcgattgcga ccgcgataag gttcacgctg
    10081 cctgcaatca cgatgatgag cgcgatgaac gagacgacat agaggtacgc cctccacgcc
    10141 gtgaggccct ccggcaccct cctacctcct ccgccccgca cgcaccgcag ccccggcgcc
    10201 gaggagcgct gcggcggcga acgcggccgt aaatcctgac gcctgtgtcg gagtcggctc
    10261 accgccgctc tggatcttaa actgcccgac ctgtgcggag gacggcgacg tcggcgccgc
    10321 ggtcgggacc gggagggaga ccggcgacgt gaccttgcct gaatcgagga ccgcaccgtt
    10381 gccgaggatc acctgcggcg gcggggccag ggtgacgcgc ccggtgctgg tcgcaaaggc
    10441 cgcacccccc tcgacgagtt tgatgctgat ctcgtagtcg tagccgttcg ggaccgtgac
    10501 gcgcacctgc ttttcgtcag ctccctcccc ggcgaccgtt ccgagcggtc gggactcgcg
    10561 gacggccgtg atcccggtct gaaggttgac gacccggata tccatggcaa ggtccccgga
    10621 tgcggcggcg gcgctgttct tgatgccggt gatgatatcg agcgttatcg ggtcggcacc
    10681 gatatcgggg gtcatgacgt cgatcgcggt gacggcaagg aacgagtagg ccaacctccg
    10741 cccggccgtc gtcctgtcgg ggagagaaac gctcaccgaa cccgactccc gcatgcgccc
    10801 gtcctgccat accgtcgccc atatgtcgta acgcctgccg ttctcgagcg caagcgtgac
    10861 ctccgccttc tccgtcctgc cgtttgtgag gttgccgatc gaaagaacct tctgtgcgac
    10921 gacgatatcg gtcccggcct ccttcgcgct gatccgcagg tcgacgttgc cgacgtcggc
    10981 gtacaccgcg tcgaggtaag gtaccaccac gacggtggtg acgtttgatg gcggctgtcc
    11041 gtccgggatg ctgatatcca cgctccggat cgtcacgtag cccgccgggg gccgggacga
    11101 cgtacaccct gccgcaagca ccagggacag gagcacgatg accgttgctg caccgaataa
    11161 ccttctcata ctgcaccggg agaacctttt gagcaccaga agaaaaggtt tccggattac
    11221 ggctcccttc gcaggccggc gggggagaag acgggtgcga ttgcacctat catggcatga
    11281 gcccgcagcc cgggagtgcg ccgggaatag ggcgactccg gtcgccagat gactgcatcg
    11341 ggcccccggg atcataggtt cgataatttt cgtagtttac cattgcttta tcctcgttcg
    11401 cgcctatccc cttatgtgaa acgccagacg ggggcgcgcg cgtgggcaga tcgtattgcg
    11461 cgtgagccgg cacaccggcg gttgccagat ccgcgacgga tctgcgggct ctttcgggat
    11521 tattgcggtt ctgcagcggt tcaaaaccgc gatcccgtcc cgacgtgtag atgcgtgtca
    11581 cagaagacgt taataagaga gcatcaaagt ctgcattagg cgccgcccgg ccgaaccggc
    11641 ggagtgtgaa cggatgatac ggacagaaaa actcacgaag gtgtataacg ggaagacggc
    11701 cgtcgacggc ctggacctta cggtcgggga aggcgaggtc ttcggctttc tcggtccgaa
    11761 cggagcgggg aagagcacga cgatcctgat gctcaccggc atgatcgagc ccaccggcgg
    11821 ccggtgcctg gtcgacggga tcgaggtcgc aaaggatccg ctcgcggtga agaagatcat
    11881 cggctacctc cccgaggacg tcgggttcta cgggaacatg accggcgagc agaacctgga
    11941 ctacttcgcc cggttctacg gtatgaacgc gcg
//
LOCUS       DAOSEE010000064        11890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_2076_length_11890_cov_241.916434, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000064 DAOSEE010000000
VERSION     DAOSEE010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 11890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11890
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2076_length_11890_cov_241.916434"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            535..933
                     /locus_tag="PLI76_06320"
     CDS             535..933
                     /locus_tag="PLI76_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61681.1"
                     /translation="MGNGGPPAAFEFYIGGSVGPSLYVRLVDGRLLHEWASAGGYSGV
                     VTETSPTPEEWGRFRETVDRLGIREWEPEYVSAHSCCDVTYWHLRLETGGLKIVVRGA
                     NAYPGSPGPEVSREFREFRAAVERLIGRST"
     gene            complement(938..>1205)
                     /locus_tag="PLI76_06325"
     CDS             complement(938..>1205)
                     /locus_tag="PLI76_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOI61682.1"
                     /translation="DGGLGFALDVGHAHVNGNLGPFLEEGGAIHVHLHDNCGNRDDHL
                     ACGEGSIDFRRVLRVLPRSATKVIEPASFRQYEKSIEYLRSIVP"
     assembly_gap    1206..1305
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1374..2471)
                     /locus_tag="PLI76_06330"
     CDS             complement(1374..2471)
                     /locus_tag="PLI76_06330"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOI61683.1"
                     /translation="MRRNLIVPTLFAIIAVGNFVCPATGQESSGDPIWESGVGDRVMA
                     AALSPDGLYGAVTTERMVYLYSQNGTTLWSYPVSGGRSVAVSSGGERIAAGGDHLLLF
                     DRNGSVIWRYRSESRIQDIAITADGRTICTGADTGLLVFSLDDGETTPDVSGSFDAND
                     PIRSVSIDSGGAGIVAGADSGNVYFLGADGRLLWNYRTGDNGIHVATSRDGSTIAAAS
                     TGRTVYLLNRNGRLLWKSSMPRAITDVSISGDGSRLVLADGGISMLNRGGEIIGMYAT
                     GEEGRIRSVSAPSDTAHILAGAADGTVSMFRVQPETPPPATATEPSRPSAAPAQEQTT
                     SQQDTALPPATPVIVSACAVAALAWRRRERR"
     gene            complement(2488..4461)
                     /locus_tag="PLI76_06335"
     CDS             complement(2488..4461)
                     /locus_tag="PLI76_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HOI61684.1"
                     /translation="MKRIGSILLILLLSIATCGTAAAYPYGAGDAIVQDGLDYIRSCQ
                     HDDGGFAEAGRDTNPGTSWFAVMAIVAAGEDPHNWRVNGTSAIDYWRSAPDAINPEGT
                     AELGKMVTLIAAAGENPRAFNNHDYVAELRARMKTDGQFGDFVYTTYWGVFGLASAGE
                     DTALPVAWLKKQQNEDGGYGWMAGAESDSDDTAAVIMALVAAGEPADTPAVARAIDYL
                     GAHQMNDGSFNYGGTSSSNAASAAWVIQAIAATGEDPSSWSKNGNDPVTTLVTLQQPD
                     GSFLWTTYTADNPCGMTARAIPALLGKPYPILPGQAAPDLQEPGRSAIATPGATTVPQ
                     TTVPAAGSVAWQPVTVTDDYGEDVGLDTMPVRIVSLAPANTEILFALGLGDRVVGVTD
                     YCNYPAEATTKPKVGGYSTVNIERVVAAHPDLVMAAFGNTEEVVNRLRDLGLTVITLS
                     PDSVEGTLHDIRLIGKATGAETEAERLATSMEARIDAVKEKTKSVAKPPTVAHIVWHD
                     PIWISGSNTFQDELITLAGGVNAFPDTEGWQIASLERFITTDPEVIVVNSGTGMGTEG
                     VDLIYRYFMNEPRLKTMKAIQNNRVYIIESDLIDRGGPRLVDALEEVAADIHPDLFDA
                     DIRKNAPIPQSPGFGVIPLAFAFLAVLLIRLKR"
     gene            complement(4458..>5663)
                     /locus_tag="PLI76_06340"
     CDS             complement(4458..>5663)
                     /locus_tag="PLI76_06340"
                     /inference="COORDINATES: protein motif:HMM:NF025833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HOI61685.1"
                     /translation="GALDAAGVGYTVDDSYYQQYGSLFIDSVNGRANEGTKGWMYQVN
                     GESPGSGANTHTVGDGDKVVFFWSESMSSTPATSPDVISIKVVIPASSGGSDPDSSDG
                     EGSGATPSSTGEQPNNGETGSLSFSLGLPVGVTVDLGEWGQTFSVNTTGTTAAGETVV
                     VSGNTLTINQSGLVLTVAAKNIDEKEGVARGLIESVTAAINPIAREIEGLGTITASLI
                     LNLTGIPAADGRLDVAFNATPDAAAGNAFALAAAENGKEITALAYTMTVTRTNLENGE
                     DIAGAVIRMTISPGWVEEHGGADAIRIARSAEDGTYQVLETRVVGTDESGNLIVEAAS
                     PGGLSTFGLLAVRAAQDTHATAATSAAPGASSAAGTPAEASPLIISAIGAIPVIGTAY
                     LIARRRREQ"
     assembly_gap    5664..5763
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5764..7027)
                     /locus_tag="PLI76_06345"
     CDS             complement(<5764..7027)
                     /locus_tag="PLI76_06345"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOI61686.1"
                     /translation="MRTRQLPLFVGLIMLSLMLVGVVSASYPMFHYDAQRTGYVTQDG
                     PQTNATLWVAETAEYADGSPAVHNGKVFVPTWPDMDFVDNDPMGLVCYDAATGTELWT
                     NELGGTSIGSVSGVAIADGQVYLGGTDGRLYCIDEETGETLWTSGQIDATGYFGLSSS
                     PLVYEGTVYALSASDGVLHAFTPEGTGSWSFSTGGAVGYFTSPAAAGGKIFVAGGGNN
                     LFCIDAATHTAAWSAALPAAVKSTPVIGDGKVYATTAERLYALDASTGAEVWNASLGG
                     TSSTPAVAGETVIAGSSDGLHAYDAGTGILLWDFPGAQVGVSPVVAGNLVYAATNERT
                     GTVYAVDTRTGEEVWSYTLEAPGDGTFAAFYASSPAVSDGVLYIGAENNRLYAFGDGG
                     LPEPAFTGWSGTVMLTAGRTFTVTPFNNE"
     gene            complement(7306..8385)
                     /locus_tag="PLI76_06350"
     CDS             complement(7306..8385)
                     /locus_tag="PLI76_06350"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HOI61687.1"
                     /translation="MMRNRLLALALAAFIVSIVVVGAAGASHSDSDGVREPTPVPAAD
                     GARTVTVTDDAGKTVTIRGIPQRIVSLAPSNTEILYALGLEDRIAAVTERCDYPPATD
                     GKPKVGGFSTVNIERVIAIEPDLIFAAPANTEEVIDRLRSLGMTVVILDPQTIDDVLH
                     DIELAGRATGQEEEALMLIDGLRKRIENVTEIAAESRAAHPSVAHVIWHDPLWISGAR
                     TFQDEVIRMAGGTNAFGSIDGWSIVSLEEFITTNPDYILVSSGSGMDRDEYDAIYNYI
                     VHEPRLQRLDAVREGRIYVIDADVVSRGSPRIVDALEEVAGYLHPDTAGAGTPEAAGT
                     VQSPGFGAIVLIGALFIVFLIRTKE"
     gene            8625..8807
                     /locus_tag="PLI76_06355"
     CDS             8625..8807
                     /locus_tag="PLI76_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61688.1"
                     /translation="MGFASLAPEQVKKVQRCEEELGVTLLAYKRPTYSELNEENLRRI
                     QALEKELNLSLVAYDV"
     gene            complement(8848..10092)
                     /locus_tag="PLI76_06360"
     CDS             complement(8848..10092)
                     /locus_tag="PLI76_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI61689.1"
                     /translation="MKPIEIIDVDVSYGAKKILEAITFHAVRGEILGIVGPNGSGKTT
                     LLKAMSRVVARDNGEIRLDEQGLDTLGHRELARRIAVVPQDISIGFDYTVRDVVMMGR
                     HPHIGRFASETARDLEICDRAMRLANVADLAEASVHDISGGERQRVLIARALAQEPKI
                     LLLDEATSNLDISHQIEILNIIRGLTGTITVISVFHDLNLAAYYCDRLIMLKEKRVVA
                     VGEPRYVLTRENLNAVYGIDTLVKTHPLTGRPYILPVYGRAAKDRLYENVHVVCGGGT
                     GSDLLYALREAGFRVSTGVLNVFDTDYATATALGIPCVTEAPFAAISPGTREDLAQCI
                     DAAYAVVVTAMPIGQGNIENIRILESYPDKPVILLNSGGNCPFPDYTGGEAEAIVRRL
                     LDRGAIPVERIEEVLQALTSRA"
     gene            complement(10089..11033)
                     /locus_tag="PLI76_06365"
     CDS             complement(10089..11033)
                     /locus_tag="PLI76_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HOI61690.1"
                     /translation="MRRTTPIVILALACTLLISMACAVVLGPVSPGTLVTLPSAWMIV
                     WDVRVPRVIAAALVGCGLAVAGTAMQGLFRNPMADPYIIGTSSGGALGATLAIVLFAG
                     TGRPMFAFAGAMAATFTVYFIARKGGRIPVETLLLSGVALATLLTALLSFLMYTSGRS
                     LHQIMFWLMGGFWNISWNDVFIALPILVGGVGIYLFARDLNILALNEEDAIHLGVNVE
                     RAKQILLALSAFVTGIAVAVAGSIGFIGLITPHVMRLIVGPDHRFLFPAAALAGAILL
                     VWADTLTRTFTGDMPVGILTACFGAPFFIYLLRSRMKA"
     gene            11303..>11890
                     /locus_tag="PLI76_06370"
     CDS             11303..>11890
                     /locus_tag="PLI76_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOI61691.1"
                     /translation="MGNLNIAVLGPLGYAKDLGKKGTTSDITFYNLKKGDDTVTLIEP
                     TRYPERLAPLFYAASMADAALVVIGEITPTLGEWILMLDEARVEQGSIVLQNYLTPRD
                     LAPLLRGTVLERYEFVDADPIVLRDRLLACAHARPSVAPAPGVVGTVPIDHHFNVRGI
                     GTVVLGGVTRGGIRKHDILKVYPGEQAITVRSIQKH"
BASE COUNT         2005 a         3614 c         3776 g         2295 t
ORIGIN      
        1 atcattgccg ccctcgatgg agcgcgtttc gtcccgggcg gcggtgagcc gaaggcaggc
       61 acccgaaagc tcgttatggc gcataccggt gcggggcggg ccgttcctat ctgacctgca
      121 cccaccggaa tcatcctccc tcccggcaaa tggtgcgcgc ccctgctcgc ctcgcttgcg
      181 gcaagccttc ttgctctcac cctcctcacc atccggagcg gggagaccct tggttgcctg
      241 gttccgcagt ctgaaggaaa gcggatccac gaggcgcagc tcgcccgggc ctacgacccg
      301 gggagaggca tctccgggaa ggtccggggc ctcttccccc tcgtcatccc cctggtctcg
      361 aactccctcg gaaaagccac ggtcctcggg ctgaccatcg atctccgggg ctaccgctcc
      421 ggcaggcgga cgccgatgcg cgacctcgcc cccggcaggg ctgatgtcgc cgggatctgc
      481 cgtatggggc ttctcatcgc agggtatttg gccgtgccgg tcgtataacc tggtatgggc
      541 aacggcggac ccccggcggc gttcgagttc tacatcggcg gaagcgtcgg gccatccctc
      601 tacgtcaggc tcgtggatgg ccggctcctc cacgaatggg cgagcgccgg cggctactcg
      661 ggggtggtga cggagacgtc gcccacaccg gaggagtggg gaaggttccg ggagaccgtc
      721 gatcggctcg gtatccggga gtgggagccg gagtacgtct ccgcccactc ctgctgcgac
      781 gtcacctact ggcacctccg tctggagacg ggcgggctga agatcgttgt ccggggtgcg
      841 aatgcctatc ccggctctcc ggggccggaa gtctcgcgag agttccgaga gttccgcgcg
      901 gcggtggagc ggttgatcgg gcggagcacc tgagggttca ggggacgatc gacctcaggt
      961 actcgatgct tttttcgtac tggcgaaacg aggccggttc gatgaccttc gtcgcgctcc
     1021 gggggagaac ccggagcacg cggcgaaaat cgatgctccc ctcgccgcac gccaggtgat
     1081 catcccggtt gccgcagtta tcgtgcaggt ggacatggat tgccccgccc tcctcgagga
     1141 acggtcccag gttgccgttg acgtgggcgt ggcccacgtc gagggcgaac ccgagaccgc
     1201 cgtcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntcaag cgtccggtcc
     1321 atcaggcagc aggtggagca cccgatagaa ttcgttggag gcactgtatt ctactacctc
     1381 ctctcccttc gccgccacgc cagcgcggca acggcgcacg cactcacgat gaccggcgtt
     1441 gccgggggga gcgcggtatc ctgctgcgag gttgtttgct cctgggcggg agcggcggac
     1501 ggtcgagacg gttcggtggc ggttgccggc gggggggttt ctggctgcac cctgaacatc
     1561 gatacggtcc cgtcggccgc accggcgaga atatgcgcgg tatcggatgg ggcggagacg
     1621 gacctgatcc gcccttcttc tcccgtcgca tacatcccta tgatctctcc gccccggttg
     1681 agcatggaga tgcccccgtc cgccagcaca agtctcgatc cgtctccgga tatcgatacg
     1741 tccgtgatcg cccggggcat cgaagacttc cagagaagcc gcccgtttcg attcaggaga
     1801 tacaccgttc gcccggtcga tgctgcggca attgtcgaac cgtcgcgcga ggtggccacg
     1861 tgaatgccgt tatcgcctgt cctgtagttc cagagaagcc ggccgtctgc gccgaggaag
     1921 tagacattcc ccgaatcggc accggcaacg atgccggcac caccggagtc gatcgatacg
     1981 ctcctgatcg ggtcgttcgc atcgaacgac ccggagacgt cgggggttgt ctctccgtcg
     2041 tccagggaga agaccagaag accggtatcc gccccggtgc agatcgtacg tccgtctgcc
     2101 gtgatagcga tatcctgtat ccgcgactcc gatctatacc gccagatcac gctgccgttc
     2161 cggtcgaaga gcaggagatg gtcccctcct gccgcgatgc gctctccacc ggatgatacg
     2221 gccacgcttc tgccgccgga taccgggtaa ctccagagtg tcgtgccgtt ctggctgtag
     2281 agatagacca tcctctctgt agtgacggca ccgtagaggc cgtccggcga gagtgccgcc
     2341 gccataaccc gatcaccgac tcctgactcc cagatcggat cgccggagga ttcctgcccc
     2401 gtagccgggc atacgaagtt cccgaccgca atgatcgcaa agagcgtggg tacgatgagg
     2461 tttcgccgca ttctgcacca gcccgtccta cctcttcagc cggatcagga ggacggcgag
     2521 gaacgcaaaa gcaagcggga tcacgccgaa acctggagat tgcggtatag gagcattctt
     2581 ccggatatct gcatcgaaga ggtcggggtg gatgtcggca gcgacctcct ccagggcgtc
     2641 gaccagacgc ggcccgcccc ggtcgatgag atcggactcg atgatgtaga cacggttatt
     2701 ctggatcgcc ttcatggttt tgaggcgcgg ctcgttcatg aagtagcggt agatgaggtc
     2761 gacgccctcg gtgcccatgc cggttccgga gttgacgacg atcacctcgg gatcggtggt
     2821 gatgaaccgc tccaggctgg cgatctgcca gccctccgta tcggggaagg cgttgacgcc
     2881 gccggcaagg gtgatcaact cgtcctggaa ggtgttgctc ccgctgatcc agatcgggtc
     2941 gtgccagacg atatgggcga ccgtcggagg tttggccacg ctcttcgttt tttccttcac
     3001 ggcgtcgatc cgtgcctcca tcgatgtcgc cagccgctcc gcttctgttt ctgcgccggt
     3061 ggcctttccg atgagcctga tatcgtggag cgtcccttcg accgagtccg ggcttaaggt
     3121 gatcacggtc aggccgagat ctcgaaggcg attgacgacc tcttctgtgt tcccgaaagc
     3181 ggccattacc aggtcgggat gcgcggccac cacgcgttcg atgttcacgg tggagtaccc
     3241 gccgactttt ggtttcgtcg ttgcctctgc cgggtagttg cagtaatcgg tcacgccgac
     3301 cacccggtcg ccgaggccga gggcgaagag gatctcggtg ttggccgggg caagggagac
     3361 gatgcgcacc ggcatcgtat cgagcccgac atcctcgccg tagtcgtcgg tgacggtgac
     3421 gggctgccac gccacggagc cggctgccgg tacggttgtc tgcggcaccg tcgtcgcccc
     3481 gggcgttgct atcgcggatc gccctggttc ctggagatcg ggtgccgcct gcccgggcag
     3541 gatcgggtag ggtttgccga gcagggccgg gatcgcccgc gccgtcatgc cgcaggggtt
     3601 gtcggcggtg tatgtcgtcc agagaaacga gccgtcgggc tgctggaggg ttacaagtgt
     3661 cgtgaccgga tcgttgccgt tcttcgacca tgacgacggg tcttccccgg ttgcggcgat
     3721 cgcctggatc acccatgccg ccgaggcggc gttcgaggag gaagtcccac cgtagttgaa
     3781 gctgccgtcg ttcatctggt gtgccccgag gtagtcgatc gcccgtgcga cggcgggtgt
     3841 atctgccggt tcgcctgcgg cgacgagggc catgatcacg gcggcggtgt cgtcggagtc
     3901 gctttcggcg cccgccatcc agccgtagcc gccgtcctcg ttctgctgct tcttcagcca
     3961 ggcgacgggg agcgcggtgt cttcgccggc cgaggcgagc ccgaagactc cccagtaggt
     4021 ggtgtagacg aaatcgccga actggccgtc ggtcttcatg cgtgctctga gttcggcgac
     4081 gtagtcgtgg ttgttaaagg cgcgggggtt ctcgcccgcg gccgctatga gggtgaccat
     4141 cttcccgagc tcggctgtac cttcgggatt tatggcgtcg ggtgccgatc tccagtaatc
     4201 gatcgccgac gtgccgttca ccctccagtt gtgcgggtct tctccggcgg cgacgatcgc
     4261 catcaccgca aaccatgagg tgccggggtt cgtgtccctc ccggcctcgg caaacccgcc
     4321 gtcatcgtgc tggcatgacc tgatgtagtc aagaccgtcc tgcacgatcg catcgcccgc
     4381 tccgtagggg taggcggccg ccgtaccgca ggtggcgatg ctgagcagga ggatgagcag
     4441 gatgctgcct atccgtttca ttgctctctc ctccgcctgg cgatgaggta tgccgtcccg
     4501 atgacgggga tcgcgcctat cgcagatatg atgagcggcg atgcttctgc aggcgttcct
     4561 gctgctgagg atgcgccggg ggccgcagag gtcgcggcgg ttgcgtgggt atcctgtgcc
     4621 gctctgaccg ccagcagccc gaaggttgag aggccgccgg gcgaggccgc ttcgacgatc
     4681 aggtttccgc tctcatcggt cccgacgacc cgggtttcaa gtacctggta cgtgccgtcc
     4741 tctgcggatc gtgcgatcct gatagcatcc gcgccgccgt gctcttcgac ccacccgggg
     4801 cttatggtca tccggatcac tgctccggcg atatcctcgc cgttctccag gttggtccgc
     4861 gtgacggtca tggtgtaggc aagggcggtg atctctttac cgttctctgc agcggcaagg
     4921 gcgaaggcgt ttcctgctgc tgcgtccggg gtggcgttga aggcgacatc gagccggcca
     4981 tcggccgccg ggatgccggt gaggttgagg atcagtgagg cggttatggt tcccagcccc
     5041 tcgatctctc ttgcgatcgg gttgatcgcc gcagtgacgc tctcgatcag gcctcttgcc
     5101 acgccttctt tttcgtcgat gttctttgca gcgaccgtca gcacgaggcc gctctggttg
     5161 atcgtgaggg tgttgccgga tacgacgacc gtctcgccgg ctgcggttgt tccggtggtg
     5221 ttgaccgaga aggtctggcc ccactccccg agatcgacgg tgacaccgac cggcaatccg
     5281 agggagaacg atagagatcc tgtttcaccg ttgttcggct gctccccggt ggaggagggc
     5341 gttgcaccgg aaccttctcc atctgaagaa tcggggtcag aaccaccgga cgatgccggg
     5401 atcacgacct tgatagagat gacatccgga gaggtcgccg gggtcgaact catgctctcg
     5461 ctccagaaga agacgacctt gtcgccgtcc ccgaccgtgt gcgtattggc accactgccg
     5521 gggctctcgc cgttcacctg gtacatccag ccttttgtcc cttcatttgc tcttccgttg
     5581 acggagtcga taaagagcga tccgtactgc tgataatagg agtcgtcgac ggtatacccg
     5641 actccggcag catcgagggc cccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnntctcgtt gttgaagggg gtgaccgtga aggtccggcc ggccgtgagc atcaccgttc
     5821 cgctccaccc ggtaaacgca ggttccggta gtcctccatc cccgaaggcg taaaggcggt
     5881 tgttctcggc gccgatgtag agaacgccgt ccgataccgc aggggaggag gcatagaacg
     5941 cggcaaacgt gccgtcaccc ggggcttcga gggtgtatga ccagacctct tcgccggtcc
     6001 tggtatccac cgcatacacg gtgccggtcc tctcgttcgt cgcggcgtag acgaggttcc
     6061 cggccacgac cggggagacg cccacctgcg cacccgggaa atcccagaga aggatcccgg
     6121 tcccggcgtc gtaggcgtgg aggccgtcgg aagaccctgc gatgacggtt tcacctgcaa
     6181 cggccggcgt cgaggatgtc ccgccgaggc tcgcgttcca gacctcagcg cccgtgctcg
     6241 catcgagggc gtagaggcgc tcggccgtcg tcgcatagac cttcccatcg ccgatgaccg
     6301 gtgtcgactt cacggcggcc gggagggcgg cgctccacgc ggcggtgtgg gtggcggcat
     6361 cgatgcagaa gaggttgttt ccgccccctg cgacgaagat cttcccgccc gcggctgcag
     6421 gcgaggtgaa gtagccgacg gccccgccgg tggaaaaaga ccatgatccg gtgccttcag
     6481 gcgtgaaagc gtgtagcacg ccgtccgaag ctgagagggc gtagaccgtt ccttcgtaga
     6541 cgaggggcga tgacgagagc ccgaagtagc cggtggcgtc gatctggccg ctcgtccaga
     6601 gcgtctcccc cgtctcttca tcgatgcagt agagccgtcc gtcggtcccg ccgaggtaga
     6661 cctgaccgtc ggcgatggca acgcccgaga ccgaaccgat ggaggtgccg ccgagttcgt
     6721 tcgtccagag ttccgtcccg gtggcggcgt catagcagac aagccccatc gggtcgttgt
     6781 caacgaagtc catatccggc caggtcggga cgaagacctt tccgttatgc accgccggcg
     6841 atccgtcggc atattcggcg gtctctgcca cccagagggt ggcgttcgtc tgcggcccgt
     6901 cctgcgtgac gtagcccgtc cgttgcgcgt cgtagtgaaa catcggataa gatgcactca
     6961 ccacgccgac gagcatgagt gagagcatga taagcccaac aaagagtggc agctgccttg
     7021 ttctcatgtg tacaagtaca cttgttgtag tgtaataaaa ctttacaaaa cgattttgat
     7081 ataattaaaa attatttgtt tttttttcga tcgaccgctc cccgggtgct cgacatgccg
     7141 gtacgctgtt tccggcccga tcaagcggtt tactgttaaa aagcggagtg atatcgtcga
     7201 acccgccggt ccgggtggcg gcgcgcagcg aggttgccgc gaagagcgtg accggcatac
     7261 agagccgcgt gccgcgcctg ttctgcaata cgtgtcgtta ctgccttatt ctttcgttct
     7321 gatcaggaag acgatgaaca gcgcaccgat gagaactatc gcgccgaacc ccggcgactg
     7381 gaccgtcccg gccgcctccg gcgtacctgc cccggcggta tccgggtgga ggtagcccgc
     7441 tacttcttcc agcgcatcca cgatccgcgg gctcccgcgg ctgacgacat ccgcgtcgat
     7501 gacgtagata cggccctccc tgaccgcatc gagcctttgc aggcgaggtt cgtgtacgat
     7561 gtagttgtag atggcgtcgt attcatctct atccatgccg ctccccgaac tgaccaggat
     7621 gtagtcgggg ttcgtggtga tgaactcctc gaggctgacg atgctccagc cgtcgatcga
     7681 gccgaacgca ttggttccgc cggccatcct gatcacttcg tcctggaacg ttctggcgcc
     7741 gctgatccag agcggatcgt gccagatgac gtgggcgacg gacggatgcg cggcccggct
     7801 ctcggccgct atctcggtaa cgttctcgat gcgcttccgg agtccgtcga tgagcatcaa
     7861 agcctcttcc tcctgcccgg tcgcccttcc ggcaagttcg atgtcgtgca ggacatcgtc
     7921 gatcgtctgc gggtcgagga tgacgacggt catcccgagc gatcgcagac ggtcgatgac
     7981 ctcttcggtg ttggcgggcg cggcgaagat caggtcgggt tctatggcga tcaccctctc
     8041 gatattgacg gtgctgaacc cgcccacctt cggtttaccg tctgttgccg gaggatagtc
     8101 gcagcgctcg gtgacggcgg cgatccggtc ctcaaggccg agggcataga ggatctcggt
     8161 gttcgagggc gcaagcgaga cgatcctctg tggtatcccc cggatggtga cggtctttcc
     8221 ggcatcatcg gtcaccgtga cggttctcgc gccgtctgct gccggtaccg gggtcggttc
     8281 ccggacgccg tcggaatccg aatgcgatgc gcctgctgcg ccgacaacga cgatcgatac
     8341 gatgaacgcc gcgagagcga gtgcgagcaa tctgttcctc atcattgcaa gtatatcgat
     8401 gcagaatgat aatacgttac cccgtgcggc ctgatacggt caccggagag gttgtgcacc
     8461 cccgggggcc catgcccggc cggaatccgg cgttgcgggg gcgggaccaa acctccccct
     8521 ccggatctct ccaccccaac ggttacggcc ggagcaccgg cggactccat aacctatatg
     8581 tgcgtgacgg gcctgtaatg ctccgggtga gaaccgtgag cactatggga tttgcgagtc
     8641 ttgcgcccga gcaggtgaag aaggtgcagc gttgcgaaga agaactcggt gtaacgctcc
     8701 ttgcgtataa gagacccacg tactcggagt tgaacgagga gaacctcagg cggattcagg
     8761 ccctcgagaa ggagctgaac ctctcgctcg tggcctatga tgtctgatcg gagcctctac
     8821 cttatatttt tgtcatgttc tggcgggtca ggcccggctc gttagagcct gcaggacctc
     8881 ttcgatccgc tccaccggga tcgccccccg gtcaagcagc ctccggacga tcgcttccgc
     8941 ctcaccgccg gtgtaatccg ggaaggggca gttgccgccg ctgttcagga ggatgacggg
     9001 cttatccggg taactctcca gtatcctgat gttctcgatg ttcccctgcc cgatgggcat
     9061 cgccgtgacg acgacggcat atgcggcatc gatgcactgc gcgaggtcct cgcgggtgcc
     9121 gggagatata gcggcaaagg gcgcttcggt aacacaggga atgcccagtg cggttgcggt
     9181 tgcgtagtcg gtatcaaaga cgttgagcac gcccgtgctt acccggaacc ccgcctcgcg
     9241 gagggcgtag aggaggtcgg aacccgtacc gccgccgcat acgacgtgca cgttctcgta
     9301 gagcctatct tttgcagcac gcccgtacac cgggaggatg tagggtcttc cggtcagggg
     9361 atgggtcttt accagggtat cgatcccgta gaccgcattg aggttttcac gggtcagcac
     9421 ataccggggt tcgccgacgg cgacgaccct cttctctttg agcatgatca gccggtcgca
     9481 gtagtacgcg gcgaggttca ggtcatgaaa gacgctgatc accgtgatcg taccggtgag
     9541 gcccctgatg atgttgagga tctcgatctg gtggcttata tcgaggttgg aggtcgcctc
     9601 gtcgagcagc aggatcttcg gttcctgcgc gagcgcacgc gcgatgagca cgcgctggcg
     9661 ctccccgccg ctgatgtcgt gcaccgaggc ttcggcgagg tccgcgacgt ttgcaagacg
     9721 catggcacgg tcgcagatct ccaggtcccg ggcggtctcg gaggcgaacc gcccgatgtg
     9781 gggatgtctc cccatcatga cgacatcgcg caccgtatag tcgaatccaa tcgatatatc
     9841 ctgcgggacg accgcaatcc ggcgtgcaag ctcgcggtgt ccgagcgtat ccagaccctg
     9901 ctcatcaagc cggatctctc cgttgtcccg ggcgacgacc cggctcatcg ccttcaagag
     9961 cgtcgtcttc cccgagccgt tcggcccgac gatgccgagg atctcacccc tcaccgcatg
    10021 gaacgtgatc gcttcaagga tcttctttgc gccgtaggag acgtcgacgt cgatgatctc
    10081 aatcggtttc atgctttcat ccggctccgg aggaggtata tgaagaacgg tgcgccaaag
    10141 caggcggtca gaatgccgac gggcatgtcg cccgtgaagg tccgcgtgag cgtatccgcc
    10201 catacgagga ggatggcgcc cgcaagcgca gctgcgggga agagaaaacg gtggtcgggc
    10261 ccgacgatca ggcgcatcac gtgcggggtg atcaggccga tgaacccgat cgacccggct
    10321 acggcaacgg ctatccccgt cacgaacgcg ctcagcgcga ggaggatctg ttttgcccgc
    10381 tcgacgttca ccccgaggtg gatcgcgtct tcttcgttca gggcgaggat gttgaggtcg
    10441 cgcgcgaaga gatagatccc cacgccgccg accaggatgg ggagggcgat gaagacgtcg
    10501 ttccacgata tgttccagaa ccctcccatc agccagaaca tgatctggtg caggctcctt
    10561 cctgaggtat acatgagaaa agagaggagc gccgtgagaa gcgtcgcgag ggcgacgccg
    10621 gagaggagaa gtgtctcgac cgggatcctg cctcctttgc gggcgatgaa gtagacggtg
    10681 aacgtggcgg ccatcgcccc ggcaaatgcg aacattgggc gccccgtgcc ggcgaagagg
    10741 acgattgcaa gcgtggcccc gagcgctccg ccggacgacg tgccgatgat gtaggggtcg
    10801 gccatggggt tacgaaagag cccctgcatg gccgtacctg ctacggcaag cccgcacccg
    10861 accagcgccg cggcgatcac cctcggcacc ctgacatccc agacgatcat ccatgcgctg
    10921 ggcagggtaa ccagggttcc gggggagacc ggtccgagaa cgactgcgca ggccatgctg
    10981 atgaggagcg tgcaggcaag tgccaggatc acgatgggcg ttgttcttcg catagggtgt
    11041 agtaaggtta actttaattt gttaaaatgt atttggttaa cgccgtggtg tgcgtggagc
    11101 ccaccgcaca tccggccgca tctgcgggat ccccgggtat ggtgcaccgc atggcggttt
    11161 catgcgaaag gagtgcggag gcgcgaaagg gagcggtgcg ggggccttca ggatcgtggg
    11221 ttccccgtgc ccgggcacgt tttttccaaa agatcgtgcc tgcgttaacc tcatactctc
    11281 gggtaaccaa atgatcaggc atatgggcaa cctgaatatc gccgtgctgg gacctctcgg
    11341 ctacgccaaa gacctcggga agaaaggcac gacatccgat atcaccttct ataacctgaa
    11401 gaagggcgac gataccgtca ccctcatcga accgacccgg tatcccgaga ggctggctcc
    11461 gctcttctac gccgcatcga tggcggatgc ggctcttgtc gtgatcggtg agatcacccc
    11521 gacgctcggg gagtggatcc tgatgctcga tgaggcccgc gtggaacagg gctccatcgt
    11581 cctccagaac tacctcaccc cccgggacct cgcgccgctc ctccgcggga cggtgcttga
    11641 gcgttacgag ttcgtggatg ccgatccgat cgtgctccgc gaccgcctgc tcgcttgcgc
    11701 gcatgcccgc ccgtccgtcg cacctgcacc cggcgtcgtg ggaacggtcc ccatcgatca
    11761 tcacttcaac gtccgcggca tcgggacggt cgtcctcggc ggcgttacgc ggggcggcat
    11821 ccggaagcac gacatcctga aggtctaccc cggggagcag gcgatcacgg tgcggtccat
    11881 ccagaagcat
//
LOCUS       DAOSEE010000065        11858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_2082_length_11858_cov_229.593154, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000065 DAOSEE010000000
VERSION     DAOSEE010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 11858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11858
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2082_length_11858_cov_229.593154"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..851)
                     /locus_tag="PLI76_06375"
     CDS             complement(87..851)
                     /locus_tag="PLI76_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOI61692.1"
                     /translation="MDEQMPLGVTITEIREETPSIRTFVFDRAIDARPGQFVMVWVPG
                     VDEIPMALSSPSSITVLKVGDATAALFAMQVGDRIGIRGPYGNGFSVTGRTLAVAGGV
                     GASPLLPLIKAGQVDTFLLGARTASELLFADQIRGAATLMVATDDGTAGHHGFVTRLM
                     SRVDLADFAHICVCGPEVMMAAVLAILDREGCADRGQFSLHRYMKCGVGLCGSCCTDP
                     HGLRVCRDGPIFSGDVLLESDFGRYTRDASGRRQRI"
     gene            complement(844..1731)
                     /locus_tag="PLI76_06380"
     CDS             complement(844..1731)
                     /locus_tag="PLI76_06380"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOI61693.1"
                     /translation="MVALYPGPVDIGGVRLKNHLLLAAGILGTTGASLARILANGAGG
                     VVTKSIGPAPKEGHPGPCLIVVDGGIINAMGLPNPSAAFADELIALTGEPVVVSIFGG
                     TPEEFRTVAGWFAGKAAGLELNLSCPHAEGYGAAIGSDPALVEECTRAAASPGVPTWV
                     KLTPNVADIGEIGAAAERGGADAVVAINTVKAMRISTSLGRPVLGNRFGGLSGKAIFP
                     VAVRCVYDLYEACSIPIIGCGGISTADNVVEMMMAGASAVEIGSAVIDDVGIFGTIAR
                     DLYSHDGIAARDIVGCAHG"
     gene            1936..2802
                     /locus_tag="PLI76_06385"
     CDS             1936..2802
                     /locus_tag="PLI76_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HOI61694.1"
                     /translation="MTSLAISGVGKVGGETAFLSAALGLADEIILYDAYEPILRSQVL
                     DLQHTGIDVTVTTDTAAMRDADVFIFAAGTPRTPDIKTRADLLEANIPVVRRCSEHLR
                     DFPGVVITITNPMDANNYALWKMMGIDRERCIGFGGQLDSVRFACLLREAGIPGPAWV
                     LGEHGEHQVPLFSKTGRYVDTGERETILSRMRGASMEVIRGKGATVFGPAYHIAMLVR
                     AVLEDRRELLSCSCVLDGEYGFSGCSLAVPARIGREGILGIEEWDLDPWEAAKMTEAG
                     AFVRGLCRRFDD"
     gene            2795..5254
                     /locus_tag="PLI76_06390"
     CDS             2795..5254
                     /locus_tag="PLI76_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase"
                     /protein_id="HOI61695.1"
                     /translation="MTDPAPESRVTIPATLNDFGKIRIGIHQVEYSVGVDIPVIHIFG
                     RDASGKAVQVRVTGFRPYFYVPAGLVNDPSPPKGVDIEPGTTYRSIRGESLRRLYTRR
                     PGDVRAVRDDFRQHYEADIPFTTRFMIDCGLTAGVELPAGAVESPEGTFEVEYTDIAP
                     ADIKAPARTCIMDIECVDEHGFPEPERDPIICVTCWDSVEEDYTTLLWQPGGAAGDAP
                     ELSTNARHKVTRYPNEAAMLRGLAAYVRERDPDILSGWNFVDFDIPYILQRMARLNLP
                     GEDLARLPGQTERNAVRGRSIFDLLGAYRKMHQAQKESYRLDAIAGEELGETKVRYSG
                     TITDLWRSDPARLVEYNCRDVELCVGIDRKNNIIEFYREISRYVGCPLDRTLNSSNVI
                     DIYVLRKASGRFVLPSKGLAAGDEFEGATVFEPATGLRENVVVLDLKSLYPMAMMTIN
                     ASPETKNPDGELRAPNGIRFSREPDGLTRSILSELLVERDERKRLRNQYPFGSPEYVL
                     YDLQQNVLKVIMNSYYGVSGYTRFRLYDREIGSAVTSVGRAIIRHTRDVITDLGYTVL
                     YGDTDSCMVEVPQGSLEETIAKAKAIEARLNASYSDFAKSELNADTHYFSIKFEKVYR
                     RFFQAGKKKRYAGHLVWKEGKDVDEVDVVGFEIRRSDSPQITREVQRTVLEMILRGDA
                     FEGVQAYLRDVIRKYRRGEYSLDEVGIPGGIGKNLDSYETDDAHIRGARYANDHLGTD
                     FKRGSKPKRIYIKTVTAKYPRTDVIAFEYADQVPPEFVVDWETMLEKTLKGPISRIIE
                     PLGWDWHDVDPTRTTLFDFGM"
     gene            complement(5279..5710)
                     /locus_tag="PLI76_06395"
     CDS             complement(5279..5710)
                     /locus_tag="PLI76_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61696.1"
                     /translation="MGGVKDSTYLDVKKALARRFSPQEGWQFAWYPTYGNVQPECVLS
                     RRTAGRTERVVVGVKMASKVPVGTIEELQGQRQALAASNVDIDRAVLVVPGGASVPAV
                     PEGIEILEMGNWQIVGDRIAWSKNIERSAFHQEERVKRGLA"
     gene            5936..6562
                     /locus_tag="PLI76_06400"
     CDS             5936..6562
                     /locus_tag="PLI76_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61697.1"
                     /translation="MAEATEIGVVEAYERLAREVLNRPESIPSAIHNLLLKLAETHPG
                     AVYWIVRKFYQEYLRLYVSDTGYIGIADRYEPGLMYPGDINLYMVPESPQPGDVVQIT
                     FTDKNEVSIYVIHGRVLRCNEDRSVQVEDTSTGEDYRVVDWNILGKLVKVIPFGSVEW
                     QELFPASGIPKEWLTTSVEGNIRSLQESDASGKDEAIAELKRRLRELV"
     gene            6678..7658
                     /locus_tag="PLI76_06405"
     CDS             6678..7658
                     /locus_tag="PLI76_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61698.1"
                     /translation="MRHLIVIVLILLLIPSGVQALTFLSGDQPAVDTPIPDDVVASGG
                     VVTINAPIDSLTAAGGTVTVNAPVKNDVIAAGGTLIINGDVGGKVLAAGGDIEVNGNA
                     TNVLATGGTVRIGQGAVIRRDAVLSGGTVANEGSVVRNLTVSANNFSNTGTAGNVTFE
                     EREEPGPLLAGIFSVLAAIGFFILGLLLVGLLPGPFSTVVHEVERRPVVFTVLGFIAL
                     FVTTIVLLIVAVTIIGLPIALFGGLLFIVGMLVSPLFVAYALGDVIASRAGRRPGRIW
                     IFVLGFVILQILIFIPLFGMIVHAVAVSLGYGGLLSALRGIRQFRAGEAA"
     gene            complement(7690..9168)
                     /locus_tag="PLI76_06410"
     CDS             complement(7690..9168)
                     /locus_tag="PLI76_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOI61699.1"
                     /translation="MSERDILLALERRAGEKPEFRLSELQKDLAAGQSLEDLAGEVRP
                     QIDRLGLTLRPSDGDYVIGRRAGPLTVDDAGRERQAAFFRNPEVPETIQHLIESYITR
                     KTGKPWDDPAVLDRMQNAILVQKSRYWKEQRVSYRKAYQVLGYLAYHAPVYLVQFEHI
                     LWQIIDRGLAKPHMRILDIGTGPGVVPLAVIDLLDRIGTGSAEIYAVERSEEHLEAYN
                     ALVPAAAAAMGDRVQVKKPTRGDIGEIAAGDLPDRIDIMVFSNVLGELRHMRIEERAD
                     LVAALAEQLAPDGTIVVVEPADLANATALRQTVRAIADRGLTIHSPCSFIRGTACNPA
                     RCWTFEQKGDIRPPRLMERLAEGKDGYRFVNVDIKYSSALLRKDARVQHAYRVPPGAK
                     FARFSHLHRHVDRTINVVAALMSGNLGDDRTKVYKVCDGTPADPVYVVVPATLRGQNR
                     DALRDIAYGDIVRFHGVLVRFNKDHGAYNLVVLPGTRVEPAA"
     gene            complement(9236..9826)
                     /locus_tag="PLI76_06415"
     CDS             complement(9236..9826)
                     /locus_tag="PLI76_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOI61700.1"
                     /translation="MKPSFDILIVGIGGQGTVLASNVLGEACIIEKRTVRSAETHGMA
                     QRGGSVESHIRIDGELGSLIVPGTADLLIAFDLLEALRYRHYLPAGGKLVVNRHLVVP
                     TSVYQQNLDVPDEESILAALADLDVACIDAAGLAEEAGSVLAQNIVMLGAASRHIPLR
                     PETLEEAVRRCVPKKTVEVNTAAFRLGREAGARGTP"
     gene            complement(9823..11592)
                     /gene="iorA"
                     /locus_tag="PLI76_06420"
     CDS             complement(9823..11592)
                     /gene="iorA"
                     /locus_tag="PLI76_06420"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HOI61701.1"
                     /translation="MAVRYLLGNEGIAHACLEANVDFASGYPGTPSSEVIDILRVQED
                     RPFTVEWSTNEKVAFENALAAAWCGLRALCTMKHVGLNVAADPLMTSAYTGVTGGFVV
                     LSADDPFAHSSQNEQDTRRYAHFARLPCLDPSSVQEAHDMLRDAFALSEEFGLPVIFR
                     PTTRICHSKSDVALGEIGTEHRTAAFHRDPRQYVVIPAHTRVLHKKLNEKQQALKERL
                     VELGYNRHAVRGRTAVIASGVAAAYVQEVLPDDVSLAIVGAYPIDEEWLREFVDRHEK
                     VLVLEELSPVVEEAVRQVATRTEVVGKLTGTVPYEGEFTPATVAAALAWAGIAPTIAF
                     PAVAPVQGVPPRPPILCAGCMHRPTFYAMKKVFRDGIFPSDIGCYTLGLQLGAVDTTI
                     CMGASITVGSGIARSGETHPVVSTIGDSTFLHTGIPGLLNAVYNDADMVVVILDNRIT
                     AMTGHQPNPNTGVTATGEESTPISLEAICRSCGVSWVETVDPYDLPLLLDTFRRAKER
                     RGVRVIIAKQPCVITARRSGIKRKAYAVDPERCTGCGVCRSFGCPAIAFSEKNATITD
                     LCAGCGVCADICPSGAIGMEGRS"
     gene            11699..11771
                     /locus_tag="PLI76_06425"
     tRNA            11699..11771
                     /locus_tag="PLI76_06425"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11732..11734,aa:Ile,seq:gat)
BASE COUNT         2067 a         3805 c         3857 g         2129 t
ORIGIN      
        1 gtatccttct tcgatgatcg gatccattgt tcacctaact cttgcgtatg ccggcataag
       61 cgcaccggtt tgtcgtccgg cggggcctaa atcctctgcc gccgcccgct cgcgtcgcgc
      121 gtgtagcgcc cgaaatcgct ctcgagcagg acgtcgccgg agaagatcgg cccgtccctg
      181 cagacccgga ggccgtgcgg gtcggtgcag cacgaaccgc agaggccgac cccgcacttc
      241 atgtagcggt ggagggagaa ctggccccgg tccgcacacc cctcccggtc gagtatggca
      301 agcaccgccg ccatcatgac ctcgggcccg cagacgcaga tgtgggcgaa gtcggcgagg
      361 tccacccttg acatgagccg ggtcacgaac ccgtggtgac cggccgtgcc gtcgtcggtc
      421 gcgaccatca gcgtcgccgc accccgtatt tggtctgcaa agaggagttc tgaggccgtg
      481 cgggcgccga ggaggaaggt gtccacctgc ccggccttga taagcggcag cagcggggag
      541 gcgccgacgc cgccggcgac ggcaagcgtc ctcccggtga cggagaaccc gttcccgtaa
      601 ggaccccgga taccgatccg gtcgcctacc tgcatggcaa agagggcggc ggtcgcgtcg
      661 cccactttga ggacggtgat cgaggagggc gaggagaggg ccatcggtat ctcgtcgacg
      721 cccggcaccc agaccatgac gaactggccc ggccgggcgt cgatagcgcg gtcgaagaca
      781 aacgtcctga tcgaaggcgt ctcctctctg atctccgtta tcgtgacccc gaggggcatc
      841 tgctcatcca tgggcgcacc ccacgatatc gcgtgcggcg atgccgtcat ggctgtagag
      901 gtcccgcgca atcgtgccga agatgccgac atcgtcgatg acggcgctcc ctatctcgac
      961 ggcgctcgcg cccgccatca tcatctcgac gacgttgtcg gcggtggata tgccgccgca
     1021 cccgatgatc gggatggagc aggcctcgta gagatcgtag acgcaccgga cggcgaccgg
     1081 gaagatcgcc ttccccgaga ggccgccgaa gcggttcccg agcaccggcc ggccaaggga
     1141 cgtcgatatc cgcatcgcct tcaccgtgtt gatagcgacg acggcgtccg cacccccgcg
     1201 ttccgcggcc gccccgatct cgccgatatc ggcgacgttc ggcgtgagtt tcacccaggt
     1261 cgggactcca ggagaggctg ccgccctcgt gcactcctcc acgagagccg gatcgctccc
     1321 gatcgccgcc ccgtagccct ccgcgtgggg gcaggagagg ttcagctcaa gcccggcggc
     1381 cttaccggca aaccacccgg cgaccgtccg gaactcttcc ggcgtccccc cgaatatgct
     1441 cacgacgacc ggttcgcccg tgagagcgat cagctcgtcc gcgaacgcgg ccgaggggtt
     1501 cggcagtccc atggcattga tgatgccgcc gtcgacgacg atcaggcagg gtccggggtg
     1561 cccctccttc ggggcggggc cgatggattt cgtgaccacg ccgcctgcac cgtttgcaag
     1621 gatacgggca agcgacgccc cggtggtccc gaggatgccg gccgcaagca ggagatggtt
     1681 cttcaaccgg acgcccccga tatcgacagg gcctggatac aacgcaacca ttgggaaaag
     1741 gtgggagtga ggtattatta taccctcgcg ccaagtatta tgaagagaaa atatgtgctc
     1801 cgcgcgccgg ggcccggata ccggaccgaa agacggcagg gtgccctttc tccccgccga
     1861 accgctatcc tccgtgcagc agggccttat cacgcataca atcttttgcc gttatccaga
     1921 cacaggacag ataccatgac ctcgcttgca atatccggtg taggaaaggt ggggggtgag
     1981 acggcatttc tctccgccgc actcggtctc gccgatgaga tcatcctcta cgacgcatac
     2041 gaacccatcc tccggtccca ggtgctcgac ctgcaacaca cgggtatcga cgttacggtc
     2101 accaccgata cggcggcgat gcgggatgcc gacgtcttca tatttgcggc aggcacaccg
     2161 agaacgcccg atatcaagac ccgagcggac ctcctcgagg caaacatccc cgtggtgagg
     2221 cggtgcagcg agcatctccg ggattttccc ggggtcgtca tcaccatcac gaaccccatg
     2281 gatgcaaaca actacgccct ctggaagatg atggggatcg accgggagcg ctgcatcggg
     2341 tttggcggcc agctcgacag cgtccgattt gcctgcctcc tccgcgaggc gggtataccg
     2401 gggcccgcct gggtgctcgg cgagcacggc gagcaccagg tgccgctctt ctctaagacc
     2461 ggcaggtacg tcgataccgg ggagcgggag acaatcctct cccggatgcg gggagcaagc
     2521 atggaggtta tccgtggaaa gggcgccacg gtctttggcc ccgcatacca tatcgccatg
     2581 ctggtgaggg cagtccttga ggaccggcgc gagctcctct cctgctcgtg cgtgctcgac
     2641 ggcgagtacg gcttctccgg ctgttcgctt gccgtcccgg cccggatcgg ccgcgagggt
     2701 atcctcggca tcgaggagtg ggacctcgac ccgtgggagg cggcgaagat gacggaggcg
     2761 ggagcattcg tgcgaggcct ctgcaggagg tttgatgact gatccggcac cggagtcccg
     2821 tgtcactatc ccggctaccc tgaacgattt cggcaagatc cggatcggca tccaccaggt
     2881 ggagtacagc gtcggcgtcg acataccggt catccacata ttcggccgtg acgcctccgg
     2941 gaaggccgtc caggtcaggg tgaccggttt tcggccctac ttctacgtgc ccgccggact
     3001 ggtgaacgat ccttcccccc caaaaggggt cgatatcgag cccggcacga cctaccgctc
     3061 catccgcggc gagtccctcc gccggctcta cacccggcgc cccggcgacg tccgtgccgt
     3121 ccgcgacgac ttccggcaac actacgaggc cgatataccg ttcaccaccc ggttcatgat
     3181 cgactgcggc ctcaccgccg gcgtcgagct gcccgccggc gccgtcgaat cacctgaggg
     3241 caccttcgag gtcgagtata ccgatatcgc gccggccgat atcaaggctc ccgcccgcac
     3301 ctgcatcatg gatatcgagt gcgtggatga gcatgggttc cctgaacccg agcgcgaccc
     3361 gatcatctgc gtcacctgct gggactcggt tgaggaggac tatacgacgc tcctctggca
     3421 gcccggcggg gctgcgggcg acgcgcccga actctccacc aacgcccggc acaaggtcac
     3481 ccgctaccca aacgaggccg cgatgctccg ggggctcgcc gcatacgtca gggaacgcga
     3541 ccccgacatc ctctcgggct ggaacttcgt ggatttcgat atcccctaca tcctgcagcg
     3601 gatggcaagg ctcaacctcc cgggagagga ccttgcgcgt ctccccggcc agacggagag
     3661 gaacgccgtc cgcgggagat cgatattcga cctcctcggc gcctaccgga agatgcacca
     3721 ggcccagaag gagtcctacc ggctcgacgc gatagccggc gaggaactcg gggagacgaa
     3781 ggtccgctac agcggcacga tcaccgacct ctggcgctcc gaccctgcgc ggctcgtgga
     3841 gtacaactgc cgcgacgtcg agctctgcgt cgggatcgat agaaagaaca acatcatcga
     3901 gttctaccgc gagatctccc ggtacgtcgg gtgcccgctc gaccggacgc tgaactcctc
     3961 aaacgtcatc gatatatacg tcctccggaa ggcgtcgggc cggttcgtcc tcccctcaaa
     4021 gggccttgcc gccggcgacg agttcgaggg ggcgaccgtc tttgagcccg cgacgggcct
     4081 tcgggagaac gtggtggtcc tcgacttaaa gtccctctac ccgatggcga tgatgacgat
     4141 caacgcatcg cccgagacca aaaaccccga cggcgaactg cgggccccaa acggcatccg
     4201 gttctcccgg gagccggacg ggttgacgcg cagcatcctc tcggagctcc tcgtggagcg
     4261 ggacgagcgc aagcgcctcc gcaaccagta tcccttcggg tcgccggagt acgtcctcta
     4321 cgacctccag cagaacgtcc tgaaggtgat catgaactcc tactacggcg tatcgggcta
     4381 cacccggttc aggctctacg accgcgagat cggttcggcc gtcacgtcgg tcgggcgggc
     4441 gatcatccgg catacccgcg acgtcatcac cgatctcggc tacacggtcc tctacggcga
     4501 caccgactcc tgtatggtcg aggtgccgca ggggagcctt gaggagacga tagccaaggc
     4561 aaaggcgatc gaggcccgcc tgaacgcaag ttacagcgac tttgcaaaga gcgagctcaa
     4621 cgccgacacc cactacttct ccatcaagtt cgagaaggtc taccggcgct tcttccaggc
     4681 cggcaagaag aagcgctacg ccggccacct ggtctggaag gaaggtaaag acgtcgacga
     4741 ggtcgacgtc gtcggattcg agatccggcg gagcgattcg ccgcagatca cgcgggaggt
     4801 gcagcgcacg gtccttgaga tgatcctccg cggggatgcg ttcgagggcg tgcaggccta
     4861 cctgcgcgac gtcatccgga agtaccggcg cggcgagtac tccctcgacg aggtcggtat
     4921 tcccggcggt atagggaaga acctggacag ctacgagacc gacgatgccc acatccgggg
     4981 cgccaggtac gcaaacgacc atctcgggac cgacttcaag cggggcagca aacccaagcg
     5041 catctacata aagaccgtca cggcgaagta cccgcgcaca gacgtgatcg ccttcgagta
     5101 cgccgatcag gtgccgccgg agttcgtcgt cgactgggag acgatgctcg aaaagaccct
     5161 caaaggaccc atatcgcgga tcatcgagcc gctcgggtgg gactggcacg acgtcgaccc
     5221 gacacggacg acgctctttg atttcggtat gtaagaaaag ttctgggggg ccggcgcttt
     5281 acgcgaggcc ccttttcacg cgctcttcct ggtggaacgc gctgcgctcg atattctttg
     5341 accaggcgat gcggtcgccg acgatctgcc agtttcccat ctcgaggatc tcaatcccct
     5401 cgggaaccgc cgggacgctc gccccgccgg gcacgaccag aaccgccctg tcgatgtcga
     5461 cgttgcttgc ggcaagcgcc tggcgctggc cctgaagttc ctcgatggtg cccaccggga
     5521 ctttggatgc catcttcacg cccacgacca cccgctcggt cctgccggct gtccggcgag
     5581 agagcacaca ctccggctgc acattgccgt aggtgggata ccatgcaaat tgccaccctt
     5641 cctggggaga aaaccgccgg gcgagggctt tcttgacatc gagatacgta gaatccttta
     5701 cgccacccat atcaatatcc tctgctttac tgtatggtca cggtcgaata taagcattat
     5761 agttctctcc ttcccggcgc cccgcgaggt gcccgggacg gcggcaggcg acgttcccct
     5821 tcccggggcc ggtgcagaga cgccgggcgc aaaggccggg ccggatgccg gcccggaata
     5881 atccggagga acacttttat tctattaccg gtattatgcg gcggggtttg tcagcatggc
     5941 agaggcgact gagatcggag tggtagaggc gtatgagcgg ctggcgcgcg aggtgctgaa
     6001 tcggccggag agcataccga gcgccatcca caacctcctc ctgaaactgg ccgagaccca
     6061 tcccggggcg gtctactgga tcgtccggaa gttctaccag gagtacctcc ggctctacgt
     6121 ctccgatacc gggtacatcg gtatcgcgga caggtacgag ccgggcctga tgtacccggg
     6181 cgacatcaac ctctatatgg ttcccgagag cccgcagccg ggcgacgtgg tccagatcac
     6241 cttcaccgac aagaacgagg tgagcatcta cgtcatccac ggccgggttc tcaggtgcaa
     6301 tgaggaccgg tcggtccagg tcgaggatac cagcaccggg gaggactacc gggtcgtcga
     6361 ctggaacatc ctcggcaaac tggtcaaagt catcccgttc ggatccgtcg agtggcagga
     6421 gctcttcccg gcgtccggca taccgaagga gtggcttacc acgtcggtcg aggggaacat
     6481 ccgctcgctc caggagtcgg acgcttccgg gaaggacgag gcgatcgcag agctcaaacg
     6541 ccgcctccgg gaactggtgt aggagcagag gctccaggac gcctcttctt gctgccggaa
     6601 aggtaattcc gggttctcct ccagccggtc tcttcatcac gcgaatcttt ttaaatgggg
     6661 tatgctctcc tccccccatg agacacctta tcgtgatcgt ccttatcctg ctgctcatac
     6721 cgtccggcgt tcaggcgctg acgtttctca gcggcgatca acccgcggtt gataccccca
     6781 tacccgatga tgtggtagca tcgggagggg tcgtgaccat caacgccccg atcgatagcc
     6841 tgacggccgc cgggggcacg gtgacggtga acgcgccggt caaaaacgac gtcatcgccg
     6901 cgggagggac cctgatcatc aacggcgacg tcggcgggaa ggtgcttgcc gccgggggcg
     6961 atatcgaggt gaacgggaac gcaacgaacg ttctcgccac gggaggcacg gtgcggatcg
     7021 ggcagggcgc ggtcatccgg cgggatgcgg tgctcagcgg cgggacggtc gcgaacgagg
     7081 gatcggtcgt gcgtaacctc accgtctcgg caaacaactt cagtaatacc ggcaccgccg
     7141 ggaacgtgac gttcgaggag cgcgaggagc cggggccgct gctggccggc atattttcgg
     7201 tgcttgccgc gatcgggttc ttcatcctcg gcctcctcct cgtcgggctc cttcccggcc
     7261 cgttctcgac ggtggtccac gaggtcgaga gacgcccggt cgtcttcacg gttctcgggt
     7321 tcatcgctct cttcgtgacg acgatcgtcc tcctgatcgt cgccgtcacc atcatcgggc
     7381 tccccatagc gctctttggc gggttgctct tcatcgtcgg catgctggta tcgccgctct
     7441 tcgtcgcgta cgccctcggg gacgtcatcg cctcgcgggc agggcggagg ccgggacgca
     7501 tctggatatt cgtcctcggg ttcgtgatct tgcagatcct catcttcata ccgctgttcg
     7561 ggatgatcgt ccatgccgtc gcggtcagcc tcggctacgg cgggctgctc tccgcgctca
     7621 ggggcattcg ccagttccgt gcaggcgagg ctgcgtgatc ggccggcgag ggttccgggg
     7681 ggagagggcc tatgcggccg gctctacccg ggtccccggc aggacgacca ggttgtatgc
     7741 gccgtgatcc ttgttgaacc ggacgaggac cccgtggaac cggacgatat caccgtaggc
     7801 gatatcgcgg agcgcatcgc ggttctgccc gcggagggtc gcggggacca cgacgtagac
     7861 cggatccgcg ggcgttccgt cgcagacctt gtagaccttc gtccggtcgt cgccgaggtt
     7921 ccccgacatc agggcggcga cgacgttgat cgttctatcg acatgccggt ggaggtggga
     7981 gaaacgggcg aacttcgccc ccggcgggac acggtaggcg tgctgcaccc gggcatcctt
     8041 tcgcaggagc gccgaagagt acttgatgtc gacgttcacg aaccggtagc cgtcttttcc
     8101 ctccgcgagc cgctccatca gacggggcgg ccggatgtcg cccttctgct cgaatgtcca
     8161 gcaccgcgcg gggttgcagg cggtgccccg gatgaacgag caggggctgt ggatggtaag
     8221 cccccggtcg gcgatcgcgc gcacggtctg cctgagagcg gtcgcgtttg caaggtcggc
     8281 gggctccacg acgacgatcg tcccgtcggg cgcgagttgt tcggcaaggg cggcgacgag
     8341 gtcggcacgc tcctctatcc tcatgtggcg gagttcgcca aggacgtttg agaagaccat
     8401 gatatcgatc cggtcgggca gatccccggc cgcgatctcc ccgatatcgc cccgggtggg
     8461 tttttttacc tgcaccctgt ctcccatcgc cgcggccgcc gccgggacga gggcgttgta
     8521 cgcctcaagg tgctcctccg agcgctcgac ggcgtagatc tctgcagaac cggtcccgat
     8581 tcgatcgagg agatcgatga cggcgagcgg cacgaccccc ggcccggtgc cgatatcgag
     8641 tatgcgcatg tggggttttg cgagcccccg gtcgatgatc tgccagagga tgtgctcgaa
     8701 ctgcaccagg tagacgggcg cgtggtaggc gaggtagccg aggacctggt aggccttccg
     8761 gtagctcacc cgctgctcct tccagtatcg gctcttctgg acgaggatgg cgttttgcat
     8821 ccggtcgagg acggccgggt cgtcccacgg cttccccgtc ttcctcgtta tgtaagactc
     8881 gatcaggtgc tggatggtct cgggcacctc agggttccgg aagaacgcgg cctggcgttc
     8941 gcgcccggca tcgtcgaccg tgagcggtcc tgcccggcgg ccgatcacgt agtcgccgtc
     9001 tgaaggcctg agggtgaggc cgaggcggtc gatctgcggg cggacctccc cggcaaggtc
     9061 ttcgagcgac tgcccggccg ccaggtcttt ctgcagttcg gagagccgga actccggctt
     9121 ttcgcccgcc cgccgttcca gcgccagaag aatatcccgc tcgctcatgg cgtatcaggt
     9181 cggcggggcg ggtaatacag gttgcgggca aaaatgatga gggggggctc tcccgtcagg
     9241 gcgtgccgcg agcccccgcc tcccgcccga gccggaaggc cgcggtgttg acctcgacgg
     9301 tctttttcgg gacgcagcgc cgcaccgcct cttcgagggt ctcgggccgg agcggtatat
     9361 gccgggacgc agcgccgagc atgacgatgt tctgcgcaag gacgctccct gcctcctccg
     9421 cgaggcctgc cgcgtcgatg caggcgacgt cgaggtcggc aagcgcggca aggatgctct
     9481 cctcgtcggg tacgtccagg ttctgctggt agaccgatgt cgggacgacg aggtgccggt
     9541 tcacgacgag tttcccgccg gccgggagat agtggcggta acggagggcc tcgaggaggt
     9601 cgaaggcgat gagaaggtcg gccgtcccgg gcacgatcag ggacccgagt tccccgtcga
     9661 tgcggatatg gctctccacc gatcccccgc gctgtgccat gccgtgggtc tcggcgctcc
     9721 ggacggtccg tttctcgatg atgcacgcct ccccgaggac gtttgaggcg aggacggttc
     9781 cctggccgcc gataccgacg atcaggatgt caaacgaggg tttcatgatc gcccctccat
     9841 gccgatcgct cccgacgggc atatatccgc gcagacgccg cagccggcgc agaggtcggt
     9901 gatcgtcgcg ttcttttcgg agaacgcgat tgcggggcag ccgaacgacc ggcagacgcc
     9961 gcagccggtg caccgctccg ggtcgaccgc gtaggctttg cgtttgatcc cgctcctccg
    10021 cgcggtgatg acgcagggct gctttgcgat gatgacgcgg acacccctgc gctcttttgc
    10081 ccggcggaag gtgtcgagga ggaggggcag gtcgtagggg tcgacggtct cgacccagga
    10141 gacgccgcag gaccggcaga tcgcttcaag cgatatgggg gtgctctctt ctccggttgc
    10201 ggtcacgccg gtgttggggt ttggctggtg gccggtcatc gcggtgatcc ggttgtcgag
    10261 tatgacgacg accatgtctg cgtcgttgta gacggcgttc aggaggcccg gtataccggt
    10321 gtggaggaac gtcgagtcgc cgatggtcga gaccaccgga tgcgtctccc ccgatcgggc
    10381 gatgccgctc ccgacggtga tcgaggcgcc catgcagatg gtggtatcga ccgccccgag
    10441 ctggaggccg agggtgtagc acccgatgtc gctagggaat atgccgtcgc gaaagacctt
    10501 cttcatggca tagaacgtcg gccggtgcat gcacccggcg cagaggatag gcggccgggg
    10561 cggcaccccc tggaccggtg cgaccgcagg gaaggcgatc gtgggtgcga tgcctgccca
    10621 cgcaagagcg gccgcgacgg ttgccggggt gaactctccc tcgtacggca ccgtgccggt
    10681 cagcttcccg acgacctccg ttctcgtcgc aacctggcgg accgcctctt cgacgaccgg
    10741 ggagagttcc tcgaggacga ggaccttttc gtgccggtcg acgaactctc tgagccactc
    10801 ctcgtcgatc gggtaggcgc cgacgatcgc aagcgagacg tcgtcgggga ggacctcctg
    10861 gacgtaggcg gccgcaacgc cgcttgcgat gacggcggtc ctgccccgga cggcgtggcg
    10921 gttgtagccg agttcgacga gcctctcttt gagcgcttgc tgcttttcgt tcagcttctt
    10981 gtgcaggacc cgcgtgtgcg cggggatgac cacgtactgc cgggggtcgc ggtggaaggc
    11041 ggcggtccgg tgttcggtgc cgatctcccc gagggcgacg tcgctctttg agtggcatat
    11101 ccgggtggtg ggccggaaga tcaccgggag gccgaactcc tcggagaggg cgaaggcgtc
    11161 ccgcagcatg tcgtgcgcct cctgtaccga cgaggggtcg aggcagggga gccgggcaaa
    11221 gtgcgcgtag cgccgggtgt cctgctcgtt ctgggagctg tgcgcgaagg gatcgtctgc
    11281 ggagaggacg acgaacccgc cggtcacccc ggtgtatgcg ctcgtcatca ggggatcggc
    11341 cgcgacgttc agcccgacgt gcttcatggt gcagagtgcc cggagaccgc accatgcagc
    11401 ggcgagcgcg ttctcaaagg cgaccttctc gttcgtggac cactccacgg tgaaggggcg
    11461 gtcttcctgc acccggagaa tatcgatcac ctctgaggac ggcgttcccg ggtagccgct
    11521 ggcaaaatcg acgtttgcct caaggcatgc gtgagcgatg ccctcattcc cgagtaagta
    11581 tcgtacagcc attgttcaac tcaactatac ttctatcatc ggttttcaac atagcgcttt
    11641 ggatgcacgc caaaacaatt aagtgctatt atggcggata ttgtagggta gtcctattgg
    11701 gcccgtagca tagccggtgg tgcacccggc tgataaccgg gaggtcatgc gttcgaatcg
    11761 catcgggccc atcgttcttt tcatcaaagc gcactggatt ccagcgtagc tcaccgatgc
    11821 ccttctccat gccgcttttc tccgtaactc cagttcta
//
LOCUS       DAOSEE010000066        11827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_2093_length_11827_cov_250.300289, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000066 DAOSEE010000000
VERSION     DAOSEE010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 11827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11827
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2093_length_11827_cov_250.300289"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="PLI76_06430"
     CDS             <1..589
                     /locus_tag="PLI76_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOI61702.1"
                     /translation="RAMYLLAHAMVGILIGIVFAAIVGDRRLIALGALGAVLPDLIDK
                     PLGHIVLAGTVNYGRIYFHGLTVLSLALLAGFLIYHYRGQVGLLVVAAGMASHQVLDG
                     MWRQPVEWFWPFLGPLPQHDYPGDYFWSSILRELAQPSEWLFFLLIAGLFTVLYRREI
                     GALLTGLADPGTWRMLVLVLGLAAVAALVVGARFL"
     gene            complement(608..1459)
                     /locus_tag="PLI76_06435"
     CDS             complement(608..1459)
                     /locus_tag="PLI76_06435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOI61703.1"
                     /translation="MKDLPMPDWNEVWRRRLALNRSTPAFREGVELWSDRERARRYAT
                     RLASDHARRVAETLSDLDVAPGDRVLDIGSGPGTLALPLARAGALVTAVEPAEGMLAE
                     LRAAAEQEGITTITTVRGLWEEIDPACDIAPPYDRVVASFSLTMPDIRSAAAAMNAAA
                     SESGSVHLYWFADEPLWERIYLALWEDLHGAPYHPRPKADCLFNVLYGMGIYADVRMR
                     PMGDTITFAAVEDAVDHFTPRMGVTTLRQREILRDYFREHLIRQDGGLALPGRSIYAA
                     IRWQKQG"
     gene            1577..2062
                     /locus_tag="PLI76_06440"
     CDS             1577..2062
                     /locus_tag="PLI76_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HOI61704.1"
                     /translation="MNSVTGESMLQITVDQLARSLCASAVLGAPVETAERVIIPVAEF
                     GFGFGAGECKGGTKEGNQGGGTGTGGGGGISAIALVIVTKGVSGPEGIQVISLKKKSE
                     IAEVITTLGEAVGPHVAKAIEKGGEMIQQRRGKPSQKPEEGRPKPEEGKEIPVSGEEG
                     A"
     gene            2084..2758
                     /locus_tag="PLI76_06445"
     CDS             2084..2758
                     /locus_tag="PLI76_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HOI61705.1"
                     /translation="MAATLLFFLLLAAVIILLAILLALYLVPVTVSTVADCGRESLRG
                     TATVAWGIVGARVRADEDVQVFEVLLAGRRVMVRDIREMVAAKPEEEKKVEEQKPALP
                     VGEYVDAARDLWPHIQRIVAAIYRSLYLETLRGEVTLGLESPASTGVVYGYCTAARYA
                     LWPAEAIDLVLTPDFDHEVFEGTFTLRVQIRRPLLIVIPVVQALLQKPVRQRLRQVSG
                     RGAPGA"
     gene            2751..3005
                     /locus_tag="PLI76_06450"
     CDS             2751..3005
                     /locus_tag="PLI76_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61706.1"
                     /translation="MPEGSELVIGGARRVGDRYIIPVIRTLVLHAGRTAVVSLTPVAL
                     IIAEGECEYLIRLPGAPRSIESALDTLRGDIDRQKRECPG"
     gene            complement(3009..4154)
                     /locus_tag="PLI76_06455"
     CDS             complement(3009..4154)
                     /locus_tag="PLI76_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI61707.1"
                     /translation="MTFFDLARRNVSRHWLRSILAVTGIAIGVVAIATLGIMGNSINL
                     MFNDMVSDVGDTITVSPATRSGTTELTKRQVDDITRAVGANRAIPFATTFDSVTVGET
                     KSGAMIYAIPAKDIPFLLDMEAGAYPKDTGSGCVIGKTLAENSKDTGLKLGARATIGN
                     ETLRVVGVLEERGMGFDINPDSAIVVPYSWYSNHYDKDTYDQVIVKVRDVNDLDAVKE
                     AIEQKMNRRENVVTVMDTRGILKEVFAAVDSITVFLMGIGAVSLIVAGVSILNVMLMS
                     VTERIKEIGVLRSIGTRRSEVMRMFIYEALILGLVGAAIGGVLSFGTGYMMTAVFSGN
                     PDYFFNPTSLLYIVFGMAFGIGMSAVSGIYPAWKAAHLNPIQALRHE"
     gene            complement(4151..4819)
                     /locus_tag="PLI76_06460"
     CDS             complement(4151..4819)
                     /locus_tag="PLI76_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI61708.1"
                     /translation="MPVISFDAVTKVYPLPAGDVVALAGIDLAIEEGEFVLVMGPSGS
                     GKSTLLNVMGSLDVPTSGEVTIAGKRISRMDDDELTLMRRDHIGFIFQQFNLIPLLSV
                     VENVEYPLILKGRRDVSRERAEEVLAAVGITESHFTHKPGELSGGQQQRVAIARALIN
                     DPDFLLCDEPTGNLDSKTGTAIMELLGRMNREEGKTVIMVTHDPRMTEYADRTIRLVD
                     GRLA"
     gene            complement(4825..5973)
                     /locus_tag="PLI76_06465"
     CDS             complement(4825..5973)
                     /locus_tag="PLI76_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61709.1"
                     /translation="MAVTMRPLYFVIISLVCALAAFTGPASAGPADITVTSSSIDPPI
                     LMKGDTGTLTIAIQNNGAEPVAIRSARLFGSGVVPLSDSYPAVGELGTKNTKTFTFTV
                     RADGGEGTFYPVFVLDFVNAGSLRYPIPVQVENTPLSISVIKKPDAFSEGRTADVTIR
                     VGNPRPNAASGVQVIPQGTGFKVIPSSAFIGALAPDASGTAVFNLTPAAETTAAFQVV
                     WRNGINTHTADLILPITFDEDKRQADPVITNVEVRQEAKGYRIVGDVMNAGLESARSV
                     IISPGEPATPTDPYRIYIVGTLDPDDASPFEVTLQADAGTTEIPIVIEYRDADGNRYT
                     ATTMVGVGGTAMALSGGEDGELPLAGIVVVVLLALGIVGAIYYSWRRK"
     gene            complement(5964..6620)
                     /locus_tag="PLI76_06470"
     CDS             complement(5964..6620)
                     /locus_tag="PLI76_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HOI61710.1"
                     /translation="MEIGFLRVLFDPGRFFESRMRDEPSLKVPALIALVIGVIGAVSA
                     ALAANMFVGILPAEAQAIGVLMVGFAAVVAVVGGFLTWFIYGIVFYIISMAFKGEGSL
                     ARTLEVTGYGFLPQIFGGIIGALLSYQIIANLTLPIARSPEEIAAVTENLAHVIATDP
                     LAQIAGVVTILFLAWSANIWVFGMKHARNLSTRNAALTVGIPVGLYIIYLLIALAGWL
                     "
     gene            complement(6960..7631)
                     /gene="radC"
                     /locus_tag="PLI76_06475"
     CDS             complement(6960..7631)
                     /gene="radC"
                     /locus_tag="PLI76_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOI61711.1"
                     /translation="MKKMRDIPDHDRPREKLAARGPQSLTNAELIALLLGRGVKGRDV
                     SQIAGDVKQYLDNAKGNPSYDALLVIEGIGAAKACEIMACFELGRRYLADDEGVAGSR
                     ITKPDEVLPLVSEWRDKKQEYFFCITLNGAGEVIRRRVVTIGILNQSLVHPREVFAEA
                     ITDRAASVILVHNHPSGTLDPSTQDISITRQLIEAGSILGIRVLDHIIVTKKGYVSLK
                     ELGHM"
     gene            7829..9484
                     /locus_tag="PLI76_06480"
     CDS             7829..9484
                     /locus_tag="PLI76_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOI61712.1"
                     /translation="MELPWRSTEVWCRKGRAYCEEGQYDRAVECYDRAIRLNAGAADA
                     WYHKGLALTAAGRHQEAAGCLDQATRIDPASARSWCARGQVLYNLRQYQEAAECCSQA
                     VKLAPDSADAWLTRGHVFRSMGRTPDAIGSYDQAIAIDPGRVETWLARGTALATERQY
                     DAAIDCYDQVIALDPGNANAWYARGTIQAILSRHNDALASCDRAVAIDSNHADAWYVR
                     GCTLAVLEQYAEAINSYDRALAVRPGDADAWYNRGRALHRLERHAEANECYDRALRIK
                     PDYAGIWYHKGTALRKLKRYEPALASFDRALKENAADPGIWYQEGLILFSLKRFEKAI
                     ECFDQVLRLRTDHPDACYYRGESYYALGDYGAAVTCYRTVVRMDPENAVAWNNYGNAL
                     YRLERYEEALACYERALGVNPENQGAWSNKANVLSILTHYDEALACYDRELRTNPENA
                     DAWYNKGLALFILGCYGEAAACYARALAIDPARAEVWCARGNTLVILERYEEALDCYD
                     RVLAANPDDPGALKGKAMALIYIDRPAEAARYYERLQRLPGRS"
     gene            complement(9585..10526)
                     /locus_tag="PLI76_06485"
     CDS             complement(9585..10526)
                     /locus_tag="PLI76_06485"
                     /inference="COORDINATES: protein motif:HMM:NF019145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61713.1"
                     /translation="MKRKVIFMGFAVVALGIIAAGVGLICLQQATPGTPIIDVPSIPN
                     IIGLSGPYVLAAPFPDVNDSYPVYRTVLPDNSIAEERRIGDLFGVSGDVKKPERTDGR
                     LYYYTNSGAFQYIIPAKAYPYATNRQPDIPSDDEARAIAMDYLKERGLLSGDIHATDV
                     SVGSSFVDGSATPGGAEYILTKHVTFVGEIGGLPVHNAGIGVSVGENGEVVAVSNSLR
                     KFDPEPVRDVKIVTPEQAYQRLSAGNVMIQPLPENYDKLVVTNISLGYWMATQTEPQN
                     YIVPVYVFSCNALWGGKSEGVFRYVPAVDPSEVQDLT"
     gene            complement(10544..>11827)
                     /locus_tag="PLI76_06490"
     CDS             complement(10544..>11827)
                     /locus_tag="PLI76_06490"
                     /inference="COORDINATES: protein motif:HMM:NF039734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6345 domain-containing protein"
                     /protein_id="HOI61714.1"
                     /translation="EPPSSPIARGGLYPATAFDPASNTLILHPGWNLVSTPLWLVDGY
                     DTMQAIFAGVDTAGHSVFRYNGSSDQWESMSAQDEFRPLEGIWIYANGSYQIPLYFKP
                     TQEQTGVTNHLYSGWNVVGFYGLSQESARDTLISLRNAWVTVQGYDAETQGYEVSMIR
                     GATGSHSDRRLMFPGKAYWVSMTEEATLVPLKATKDYHDLDYPQVGVTWVDNYDFNWQ
                     HPLSQSSNTSRGFYNTLVVEGGWIPMFEYGDHEVFRGLFTSSEWGGEDWKYIDGVDVA
                     LVASHGSASAMALSDVLGTFHFVDCSWGDNDLEWIFLHGCHTTNATETVFKRPPPRTY
                     TMNGVHLVCGFETEGWDVNDGENLATLLLNGETVIDAWFNATDMTHGPPVRLRVLGES
                     KDCENDHIWGSGSVVSDEDLYVDPYVYQWQYTCLP"
BASE COUNT         2248 a         3668 c         3641 g         2270 t
ORIGIN      
        1 gagagcgatg tacctcctcg cccacgccat ggtcggcatc ctcatcggga tcgtcttcgc
       61 ggcgatcgtc ggcgatcggc gacttatcgc gctcggcgcc ctcggtgccg tcctccccga
      121 cctgatcgat aagccgctcg ggcacatcgt ccttgccggg acggtcaact acgggcggat
      181 ctacttccac ggcctcaccg tcctctccct cgccctcctc gccggcttcc tcatctacca
      241 ctaccgcggc caggtcggcc tcctcgtggt ggccgccggt atggcaagcc accaggttct
      301 cgacggcatg tggcggcagc ccgtcgagtg gttctggccg ttcctcggtc ctctcccaca
      361 gcacgactac ccgggagact acttctggag ctcgatcctg cgggagctcg cccagccgag
      421 cgaatggctc ttcttcctcc tgatcgccgg gctgtttacc gttctttacc ggcgggagat
      481 aggggccctc ctcaccgggc ttgcagaccc tggaacctgg aggatgctcg tcctcgtcct
      541 cggtctcgcc gccgtcgccg cgctggtcgt gggcgcgcgg ttcctgtgag aggagatctc
      601 ccggccttca cccctgtttt tgccaccgga tcgcggcgta tatcgatctc ccggggagag
      661 caagcccgcc atcctgccgg atgaggtgtt cacggaagta gtcccggagt atctcccgct
      721 gccgcagggt cgttacgccc atccggggag tgaagtggtc gaccgcatcc tccacggccg
      781 caaacgtgat cgtatcgccc atcgggcgca tccggacgtc tgcgtagatc cccatcccgt
      841 agaggacgtt gaagaggcag tcggccttcg gcctcgggtg atacggcgcc ccgtgcaggt
      901 cctcccagag cgcgaggtat atccgttccc agagcggttc atcggcgaac cagtagaggt
      961 ggaccgaccc ggactcggag gcagctgcat tcatcgccgc cgcggccgac cggatgtcgg
     1021 gcatggtgag cgagaacgag gcgaccaccc tgtcgtaagg cggcgcgatg tcgcaggccg
     1081 gatcgatctc ctcccagagg ccgcggacgg tggtgatcgt ggtgatcccc tcctgctccg
     1141 ccgccgcccg gagctcggca agcatcccct ccgcaggctc gactgccgtc accagcgcac
     1201 cggcccgggc aagcggcagg gcgagagtcc cgggccccga cccgatatcc agcacccggt
     1261 cgccgggagc gacgtcgagg tccgagaggg tctcggcaac ccgtcgtgca tgatcggacg
     1321 caaggcgggt agcgtaccgc cgggcccgct cccggtcgct ccagagttcg acaccctccc
     1381 ggaacgccgg agtcgaccgg ttcagggcaa gcctcctccg ccagacctcg ttccaatccg
     1441 gcatgggaag atccttcatc ggacaatcgg tcgcctccga agcataaaca ggtatccata
     1501 cgcgccctgt gggcatccgt gtggcccggg ggatacccca accattatat atgaggccgg
     1561 agagggaata cttgtggtga atagtgtgac tggcgaatcc atgctccaga taaccgtcga
     1621 tcaacttgcg agatcactct gtgcgagcgc cgtcctcggt gccccggtcg agactgctga
     1681 acgggtcatc ataccggtcg ccgaattcgg gttcgggttt ggtgccggag aatgcaaagg
     1741 agggacgaaa gaaggtaacc agggcggcgg aaccgggacc ggcggcgggg gcggcatatc
     1801 ggccatcgct ctggtcatcg tcaccaaggg cgtatccggc cccgaaggta ttcaggtcat
     1861 atcgctgaag aagaagagcg agatcgccga ggtgatcacg acgctcgggg aggcggtcgg
     1921 cccgcatgtc gcgaaagcga tcgagaaggg cggcgagatg atacagcagc gtagaggcaa
     1981 accgtcccag aagccggaag aaggaagacc gaagccggaa gaggggaagg agatcccggt
     2041 cagcggtgag gaaggggcat aaagtcctct ttttctccag aacatggccg cgacccttct
     2101 cttcttcctc ctgcttgccg ccgtcatcat cctccttgcg attctgcttg ccctgtacct
     2161 ggtcccggtg accgtctcga cggtcgccga ctgcgggcgg gaaagcctcc gcgggacggc
     2221 aaccgtagcc tggggcatcg tgggggcgag ggtccgggcc gacgaggatg tgcaggtgtt
     2281 cgaggtcctc cttgccggcc ggcgggtcat ggtccgggat atccgggaga tggtggcggc
     2341 aaaaccggaa gaagagaaga aggtggagga gcaaaaaccg gccctcccgg tgggggagta
     2401 cgtcgacgcc gcccgcgacc tctggcccca tatccagagg atcgttgccg ccatctaccg
     2461 gtctctctac cttgagaccc tccgcgggga ggtcaccctc ggcctcgaga gccccgccag
     2521 taccggcgtc gtctacggtt actgcaccgc cgcccgttac gccctctggc cggcggaggc
     2581 gatcgatctc gtgctgaccc cggactttga ccatgaagtc ttcgagggaa cgttcacgct
     2641 cagagtgcag atccgccgcc ccctcctgat cgtcataccg gtcgtgcagg ctctcctgca
     2701 gaaaccggtg cggcaacggc tccggcaggt ctcgggaaga ggtgctccgg gtgcctgaag
     2761 ggagcgaact tgttatcggc ggcgcccgga gggtcggcga ccggtacatc atccccgtca
     2821 tcaggacgct cgttctccat gcaggaagaa ctgccgtggt cagcctgacg ccggtcgcgc
     2881 tcatcatagc cgaaggagag tgcgagtacc tcatccgcct cccgggcgcg ccccggtcga
     2941 tcgagagcgc actcgatacg cttcgcggcg atatcgaccg ccaaaaaaga gagtgtccgg
     3001 ggtagccgtc actcgtggcg gagagcctgg atcgggttta agtgcgccgc cttccaggcc
     3061 gggtagatgc cggagaccgc gctcatcccg atgccgaagg ccatcccgaa gacgatgtag
     3121 agaaggcttg tcggattgaa gaagtagtcg gggttcccgg agaagacagc cgtcatcatg
     3181 tatccggtgc cgaaactgag cacgcccccg atggcggcac ccacgagccc gaggatcagg
     3241 gcctcgtaga tgaacatccg catcacctcg ctccggcggg tgccgatgct ccgcaggacc
     3301 ccgatctcct tgatccgttc ggtgaccgac atcagcatca cgtttaagat cgagactccg
     3361 gcgacgatga gcgataccgc gccgataccc atcagaaaga ccgtgatcga gtcgacggcc
     3421 gcaaagacct ctttcaggat cccccgggta tccatgacgg tgacgacatt ctcccgccga
     3481 ttcatcttct gttcgatcgc ctccttgacc gcatcgaggt cgttgacgtc gcggaccttc
     3541 acgatgacct ggtcgtacgt atctttatcg tagtggttcg agtaccacga gtagggcacg
     3601 acgatggcac tatcagggtt gatgtcgaac cccatccccc gttcctcgag cacgcccacg
     3661 acccggaggg tctcgttgcc gatcgtggcc cgggcaccga gtttgaggcc ggtatccttg
     3721 ctgttctcgg caagcgtctt tccgatcacg cacccggaac cggtatcctt cgggtatgct
     3781 cccgcctcca tatcgagcag gaaggggata tctttggccg ggatcgcgta gatcatggcg
     3841 ccgcttttgg tctcgcccac ggttaccgag tcaaacgtgg tcgcgaacgg tatggcccgg
     3901 ttggcgccga ccgcccgggt gatatcgtcc acctgccgtt tggtgagttc cgtggttccc
     3961 gagcgggtcg ccggggagac ggtgatggta tcgccgacat cgctgaccat atcgttgaac
     4021 atgaggttga tgctgttgcc catgataccg agcgtggcga tcgcgacgac gccgatggcg
     4081 atcccggtga ccgcgaggat cgacctgagc cagtggcggc tgacgttccg gcgggcaagg
     4141 tcgaagaagg tcatgccagc ctcccatcca ccagccggat cgtgcggtcg gcgtactcgg
     4201 tcatccgggg atcgtgcgtg accatgatga cggtcttgcc ctcctcgcgg ttcatccggc
     4261 cgaggagctc catgatcgcc gtcccggtct tcgagtccag gttcccggtc ggttcgtcgc
     4321 agaggaggaa gtcgggatcg ttgatgagcg cccgggcgat cgccacccgc tgctgctgtc
     4381 cgcccgagag ttcgccgggt ttatgggtga agtgcgactc ggtgattccg accgccgcga
     4441 gaacctcctc tgcccgctcg cgggatacat cccgccggcc tttcaggatg agcgggtact
     4501 cgacgttctc gaccaccgag aggagcggta tgaggttgaa ctgctgaaag atgaacccga
     4561 tgtggtcccg ccgcataagg gtcagttcgt cgtcgtccat cctgctgatc cgctttcctg
     4621 cgatggtgac ctctcctgat gtcgggacgt caagcgaccc catgacgttt aagagcgtcg
     4681 acttccccga cccggagggg cccatgacca ggacgaactc accctcttcg atagcgagat
     4741 cgatcccggc aagcgccacg acatcgccgg ccgggagggg gtagaccttc gtgaccgcat
     4801 cgaaactgat gacgggcatc gctgttactt ccgtctccag gagtagtaga tggcgccgac
     4861 gatcccgaga gcgagaagca cgacgacgac gatgccggca agcggcagct cgccgtcctc
     4921 tccgccggac agcgccattg cggtgccgcc gacgccgacc atcgtcgtcg ccgtgtagcg
     4981 attgccatca gcgtcccggt actcgatgac gatcgggatc tccgttgttc ccgcgtcggc
     5041 ctggagtgtc acttcaaacg gagacgcatc gtcgggatcg agggtcccga cgatgtagat
     5101 ccggtacgga tcggtggggg tcgccggctc gcccggggag atgatcaccg atcgcgcgga
     5161 ctcaaggcct gcgttcatga catcgcccac gatccggtat ccttttgctt cctgccggac
     5221 ctcgacgttg gtgatgaccg ggtccgcctg ccgtttgtcc tcgtcgaacg tgatcggcag
     5281 gatgaggtcg gcggtatggg tgttgatgcc gttgcgccag accacctgga aggcggcggt
     5341 cgtctccgct gccggggtca ggttgaagac ggcggtcccg gaggcgtcgg gcgcaagagc
     5401 gccgatgaac gcgctcgacg ggatgacctt aaaacctgtt ccctgcggga tcacctggac
     5461 gccggaggcg gcgttcgggc gggggttccc cacccggatg gttacgtcgg ccgtcctccc
     5521 ctcggagaag gcatcgggtt ttttgataac cgatatgctg agcggggtat tctcgacctg
     5581 gaccgggatc gggtagcgca ggctgccggc gttgacgaag tcgaggacga agacggggta
     5641 gaacgtcccc tccccgccgt ccgcacgaac ggtgaaggtg aacgtcttcg tgtttttggt
     5701 accgagctcc ccgacagcgg ggtaggagtc gctcagcggc acgacaccgc tcccgaagag
     5761 ccgggcgctc cggatagcga ccggttcggc accgttgttc tggatcgcga tcgtgagcgt
     5821 cccggtatcc cctttcatca ggatcggcgg gtcgatggag gaggaggtca cggtgatgtc
     5881 cgcggggccg gcagaggccg gtccggtgaa tgccgcgagg gcgcagacaa gtgatatgat
     5941 gacaaagtag agcggtctca tggttacagc catccggcga gcgctatgag gagatagatg
     6001 atgtagaggc ccacgggtat cccgacggtg agggccgcat tccgcgtgga gaggttgcgc
     6061 gcatgtttca tgccgaagac ccatatgttc gcgctccagg ccaggaagag gatggtcacg
     6121 accccggcga tctgcgcgag cgggtcggtt gctatgacgt gcgcgaggtt ctccgtcacc
     6181 gccgcgatct cttcagggct cctcgcgatc gggagcgtca ggttcgctat gatctggtag
     6241 gagagaagag ccccgatgat gccgccgaag atctggggca ggaacccgta accggtgacc
     6301 tcgagcgtcc gcgcaagcga tccctctccc ttgaaggcca tcgatatgat gtagaagacg
     6361 atgccgtata tgaaccatgt caaaaacccc ccgacgacgg caaccaccgc ggcgaagccc
     6421 accatgagca ccccgatggc ctgggcctct gcggggagta ttccaacgaa catgtttgcc
     6481 gccagtgcag ccgagaccgc accgatcacc ccgataacga gcgcgatcag cgccggcacc
     6541 ttcaggctcg gttcatcccg catgcggctc tcgaaaaaac gcccgggatc gaagagtacc
     6601 cggagaaatc caatttccat gatagatatg cctcctgtat ttctgttata caagagggga
     6661 taaacttttt cggaaacacg gaagcaaaaa ttcagggatc ttcccaaatc tccggagaat
     6721 tgcgagggta ttaccccaaa cccggacctg aggtggaccg caccgggatc gaggtgccgg
     6781 cgaggatatg ccgcccgcat cgcccggggg aagagcggcg gcgggggcgc ccctgacgcc
     6841 taacgtctca aaatcccgca tgcggttctt cttcgcggca acgcgagagg ctgtctcacc
     6901 gctcatatac ctgaacacgc gcattgccgc gaagtccggg agatgggtcg agcactcccc
     6961 tacatgtgcc cgagctcttt gaggctcacg taacccttct tcgtgacgat gatgtggtcg
     7021 agcacccgta tgccgaggat cgacccggcc tcgatgagct gccgggtgat gctgatatcc
     7081 tgggtggagg ggtccagggt gcccgaggga tggttgtgga ccagtatgac cgatgcggcg
     7141 cggtcggtga tcgcctcggc aaagacctcg cggggatgga cgaggctctg gttcaggata
     7201 ccgatggtga cgacccggcg tctgatcacc tccccggcac cgttgagcgt tatgcagaag
     7261 aaatattcct gtttcttgtc ccgccactcg ctgaccagcg ggagcacttc atcgggcttg
     7321 gtgatccggc tcccggcaac gccctcatcg tctgccaggt atcgccgccc gagctcgaag
     7381 caggccatga tctcgcaggc tttcgccgcc ccgatgccct ctatcacgag gagggcatca
     7441 tacgaggggt tgcctttggc attatccagg tattgtttca catctccggc aatctgcgag
     7501 acgtcgcgcc cctttacacc tcgcccgagg aggagggcaa tcagttcggc attggtgagc
     7561 gactggggcc cccgtgctgc gagcttctca cgcgggcgat cgtggtcggg tatatcgcgc
     7621 atcttcttca tgctcattcg gccctgcgag gttcttcatc tccggttttg attcggaagc
     7681 atgtatacgt tgtcaatcgg ttcgccgcac cgggtgagcg caacgcgaga gcagggcgag
     7741 gggaggcgtt aggttctctg ttgggcatgc agaaaaccgg gggatactac cgaaaccctt
     7801 ttgtctgaga actgctatct ctcacaaaat ggagctgccc tggaggagca ccgaggtctg
     7861 gtgcaggaaa ggacgggcgt attgcgagga ggggcagtac gaccgggccg tcgagtgcta
     7921 cgaccgggcg atcaggctga acgccggcgc cgccgatgcc tggtaccata aggggctcgc
     7981 tctcaccgct gccgggcgcc accaggaggc ggccggatgt ctcgaccagg ccaccaggat
     8041 cgatccggca tccgcccgct cctggtgcgc ccgggggcag gtgctctaca acctccggca
     8101 gtaccaggaa gccgccgagt gctgcagcca ggcggtgaaa ctcgcgcctg actccgcgga
     8161 cgcctggctt acccgggggc acgtcttcag gagcatgggg cgaacccccg atgcgatcgg
     8221 gagctacgat caggccatcg ccatcgatcc gggccgggtg gagacctggc ttgccagggg
     8281 aacggcgctt gccaccgaac gacagtatga tgcggcgatc gactgctacg atcaggtcat
     8341 cgcgcttgac cccgggaacg caaacgcctg gtatgccaga ggaacgatcc aggccatcct
     8401 ctcccggcac aacgacgccc ttgcatcctg cgaccgggcc gtcgcgatcg actcaaacca
     8461 cgccgacgcc tggtatgtca ggggatgcac gcttgcggtg ctggagcagt atgcggaggc
     8521 gatcaactcc tacgaccggg cgcttgccgt ccgtccgggg gatgccgatg cctggtacaa
     8581 ccggggccgg gcgctgcacc gcctcgagcg ccatgcggag gcaaacgagt gttacgaccg
     8641 ggcgctccgg atcaagcccg actacgccgg aatctggtac cataaaggca cggcactgag
     8701 gaaactgaaa cgctacgaac cggcgctcgc ctcttttgac cgggcactca aggagaacgc
     8761 cgccgacccg gggatatggt atcaggaggg gctcatcctc ttcagcctga agcgctttga
     8821 gaaggcgatc gagtgcttcg accaggtgct caggctccgc accgatcacc cggatgcatg
     8881 ctactaccgt ggcgagagtt actacgccct cggggactac ggggcggcgg tcacctgcta
     8941 ccggaccgtg gtccggatgg acccggagaa cgccgtcgcc tggaacaact acggaaacgc
     9001 gctctaccgg ctcgagcgtt acgaagaagc ccttgcctgc tacgaacggg cgcttggagt
     9061 caaccccgag aaccagggcg cctggagcaa caaggcaaac gtcctctcga tcctcacgca
     9121 ttacgatgaa gcactcgcct gctacgaccg ggagctccgg acaaacccgg agaacgcaga
     9181 cgcgtggtac aacaaaggtc ttgcgctctt cattctcgga tgttacggcg aggctgccgc
     9241 ctgctatgcg cgagcactcg ctatcgatcc cgcgagagcg gaggtctggt gcgcccgggg
     9301 caacaccctc gtcatcctgg agcgttacga ggaggcgctc gactgctacg accgggtgct
     9361 cgccgcaaac cccgacgacc ccggggcgct caaggggaag gcgatggccc tcatctacat
     9421 cgaccgcccc gccgaggcgg ccaggtacta cgagaggctg cagcggctgc cggggcggtc
     9481 ctgagcgcgg gatcccctat acccgcgcta tccgcccgac ggcttccatc aggttctcca
     9541 tcgacgtcgc gtaggagagc cggagccacg ggcagggaat cgcatcaggt gagatcctgc
     9601 acctccgacg gatcgactgc cggtacatat cggaagactc cttccgactt gccgccccac
     9661 agtgcgttgc acgaaaagac atacaccggc acgatgtagt tctggggttc ggtttgtgtc
     9721 gccatccagt agccaagcga gatgttggtc accacgagtt tgtcgtaatt ctctgggagt
     9781 ggttggatca tgacgttccc cgcagagagt cgctgatatg cctgctccgg agtcacgatc
     9841 ttcacatcgc ggaccggttc cggatcgaat ttcctgagag aattcgagac agctaccacc
     9901 tcgccgtttt ctccgaccga tacgccgatc cctgcgttat gcacggggag gcccccgatc
     9961 tctcccacga atgttacatg tttcgtgagg atatactccg ctccaccagg ggttgcgctt
    10021 ccatctacaa acgaagatcc gacactcaca tccgtagcgt gaatgtcacc ggaaagaagt
    10081 ccgcgttcct tcaagtagtc catcgcgatc gcacgagcct cgtcgtcgga cggaatgtcc
    10141 ggctgcctgt tagtggcgta agggtacgcc tttgccggga tgatatactg gaacgctccg
    10201 gagttcgtgt aataatacaa ccttccatcg gttctttcag gctttttcac gtctcccgat
    10261 accccgaaca gatcgccgat gcgccgttcc tccgcgatgc tgttgtcggg gagaacagtc
    10321 cggtataccg ggtacgaatc gttcacatcc gggaacggtg cggcgaggac atagggtccg
    10381 gataatccaa tgatattcgg aatcgaagga acgtctatga ttggtgttcc tggcgttgcc
    10441 tgttgcaggc agatcaaccc gactcctgcg gcgatgattc ccagtgcaac aactgcgaat
    10501 cccataaata ttacttttct cttcattttc tactccaaaa tttttaagga aggcaagtat
    10561 attgccactg gtagacatat gggtcgacat acagatcttc gtctgatact accgatccgc
    10621 tgccccaaat atggtcgttt tcgcagtcct tactctcacc gaggactctt agtctgacgg
    10681 gaggcccatg cgtcatgtca gtcgcattga accatgcatc tatgactgtc tctccattca
    10741 ggagcaaagt agcaagattt tccccgtcat tcacgtccca accttcggtc tcaaatccgc
    10801 agacaagatg aactccgttc attgtatatg tccttggtgg agggcgttta aagaccgttt
    10861 ctgtggcgtt tgtggtatga cacccgtgga ggaatatcca ttccaggtca ttgtcccccc
    10921 atgagcagtc tacgaagtga aaggttccaa gaacgtcact taaagccatc gcactagcgc
    10981 tgccatgact tgcgacaaga gcaacgtcaa ccccatcgat gtacttccaa tcctcgcctc
    11041 cccattcact tgatgtaaat agaccccgaa aaacctcgtg atctccgtac tcaaacatcg
    11101 ggatccagcc tccctctaca acaagcgtgt tgtagaatcc gcgagaggta ttgctgcttt
    11161 gcgagagtgg atgttgccaa ttaaaatcgt aattatccac ccacgtcacc ccgacctggg
    11221 gataatcaag atcgtgataa tccttcgtcg ctttcagagg aaccagagtt gcttcttccg
    11281 tcatggacac ccagtacgcc tttcccggga acatcaggcg ccggtcgctg tggctgcctg
    11341 ttgcgccgcg gatcatgctg acctcgtaac cctgcgtttc cgcatcataa ccctgtaccg
    11401 ttacccacgc gttcctgagg gaaatgagcg tatcccgtgc agattcctgg ctcaaaccat
    11461 agaatccgac aacgttccag ccggaataga ggtggtttgt cactcccgtc tgctcttggg
    11521 tgggcttgaa gtagagcggg atctggtacg acccgtttgc atagatccat atcccctcga
    11581 gcggcctgaa ctcatcctgg gcgctcatcg attcccactg gtcgctcgag ccgttatatc
    11641 ggaaaaccga gtggccggcc gtgtccaccc cggcgaagat cgcctgcata gtatcatacc
    11701 cgtcgacaag ccagaggggc gtcgagacaa gattccatcc cggatgcagg atcagcgtgt
    11761 tcgacgccgg atcgaaggcg gttgccggat acaacccgcc ccgcgctatc ggcgagctcg
    11821 gtggctc
//
LOCUS       DAOSEE010000067        11703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_2118_length_11703_cov_221.941707, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000067 DAOSEE010000000
VERSION     DAOSEE010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 11703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11703
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2118_length_11703_cov_221.941707"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /locus_tag="PLI76_06495"
     CDS             <1..1281
                     /locus_tag="PLI76_06495"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HOI61715.1"
                     /translation="PVLQVALDLLELDRAVEIADEAVRGGADWIEAGTPLIKSEGMRA
                     VRTLRERFPGREIVADMKIADTGAVEVEMAAKSGAGIVCILADADDTVVAEAVRSARK
                     YGVRIMADLINIDDPVARSREVAALGVDYINAHVGIDQQMIGRSSLDLLRRLAGEVDI
                     PIAVAGGLDATTASGAVAAGASIVIVGGNIIKAADVTAAAQRVRAAIDRPDIRPREEE
                     SPDVAIRRLLEAVSAPNVTDAMHRKGAMAGVVSLCGRVKAVGRAVTVRTVAGDWAKPV
                     EAIDVAGPGDVLVISNDGGTHIAPWGELATHSAKNRGVSGVVIDGAVRDVDDIREMAF
                     PVFARATVPNAGEPKGLGEINAEIACCGQEVRPGDWIVADESGVVVVPRERAYEVARR
                     AMEVKKTEERIREEIRRGKTLSEVSELLKWEKRH"
     gene            complement(1339..1770)
                     /locus_tag="PLI76_06500"
     CDS             complement(1339..1770)
                     /locus_tag="PLI76_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOI61716.1"
                     /translation="MEKSYIIKQISIFSENRPGRLAAVAGALRDAGINIFAFSIAEAN
                     GFGVVRALVNRPEDAHRTLTGLGFRVSFTDVIGVKMRDEPGGLSEVASVLGDAGINIE
                     YAYAYSGKDAAVLILRVDKVEDAVRQILDHGGELLKASLFR"
     gene            complement(1770..3071)
                     /locus_tag="PLI76_06505"
     CDS             complement(1770..3071)
                     /locus_tag="PLI76_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOI61717.1"
                     /translation="MAPWNRQIEVMPTDELRQLQYRLAKSLIRRLYETSEFYRRRMKE
                     HDVRPDDIGSLADIGKLPFMYKGDLRDNYPDGLFCAQKDELVRYHVSSGTTGKPTVVG
                     YTRQDIENWSESLARAFSSCGLGRGDVLQVSYGYGLFTGGLGVHYGAERVGATVLPTS
                     VGNTERQIELMQDLHTTAIACTPSYLLHMGEVAEKMGVSIKNDTDLRIGILGAEPWSE
                     QMRGRLEEWLGIRAYDIYGTSELSGPMFTECSEQQGIHIWGDIALVEVVDPETGEALP
                     PGERGEMVVTMLQKEAFPLVRYRIGDITVLDEEPCACGRTHPRIRRIQGRVDDMLIVR
                     GINVFPSQVEHVLLEIPEVGRHFQIVIDRKGALDTMLVRVEVSEEAFSDKITELMVIK
                     KAVEHRLKTTLNVAVGVELVEPGTLPRFEGKSKKVVDRRSL"
     gene            complement(3071..4360)
                     /locus_tag="PLI76_06510"
     CDS             complement(3071..4360)
                     /locus_tag="PLI76_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOI61718.1"
                     /translation="MFWNRAIETIRPEELADLQLKRLKWALRQAQEVGLYQRKFREAG
                     ISPDDIRTLDDVEKLPFTYKKELQAGYPFGLFAVPMREIVRIHTTSGTTGKPTVVGYT
                     RQDLDNWADLIARNMTMIGLGVDDVFQNAVNYGLFTGGLGFHYGAEKIGMTVIPSATG
                     NTRRQIEMIEDFGVTAIHCTPSYALHLAEVAESMGKTLDTLKTGIFGAEPWSENTRVE
                     LERRLGVKAYDSYGLSEMYGPGVAFECPEHNGLHIWHDCYLTEVIDPVTGERLAPGER
                     GEMVITPLVKEALPLVRYRTGDITRLLEGECACGRGQKIERITGRSDDMLVIRGINIF
                     PSQIEHVLRSLPEVGDQFMVYVDRVRHLDEMTIEVEMTRAGFSGELQDLARVQKKIVG
                     ELHSTLGLRTAVKLVEPGSLPRFEGKAKRVIDRRGAV"
     gene            complement(4495..5529)
                     /gene="wtpA"
                     /locus_tag="PLI76_06515"
     CDS             complement(4495..5529)
                     /gene="wtpA"
                     /locus_tag="PLI76_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tungstate ABC transporter substrate-binding
                     protein WtpA"
                     /protein_id="HOI61719.1"
                     /translation="MNRSPVLAVLILAVLAASAGCADAGPERTILMVIPAGSLLVPLE
                     DIEAAFESQHPDIDVRVEGHGSIQAIRQVTDLGREVDVVLVADASLIPDMMYLPMGAG
                     EGNYTDWYIPFAENEVVIAYTGRSAGAGEITAENWYRILSRPDVRVGFSNPMLDACGY
                     RAIMVTALAEEYYGEPGLFEAIIGGSFKPPIAAVWTDGATTIALPELMRPAAEKVAIR
                     DGSIYLLSLLDAGGIDYAFEYRSVAEEHGLRWIDLPPAINLGSPEHADDYRKVRVNLG
                     FQRFRSIGSERIGQPIVYAMTIPANAPHPAEARIFVDFILDAFREGRAGWPDPVRQDP
                     GTATVYHATD"
     gene            complement(5526..6092)
                     /locus_tag="PLI76_06520"
     CDS             complement(5526..6092)
                     /locus_tag="PLI76_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOI61720.1"
                     /translation="MIPVRRISLPLILVLIGALALGAGCTDTGSVDDYPAWNLTLAGD
                     REQVLTHPEIRALPAAEGYGYSVSTVGIKTGPNRYRGVLLADLIDMAGGAGADDLIYV
                     SAEDGYLWVFDMDQVNGEGFFTFDENLREVTSPPLRVILAYEQDKEPLAYENGGPLRL
                     VVITESPDVITEGSPWVKWVDRVEVHRK"
     gene            complement(6089..6934)
                     /locus_tag="PLI76_06525"
     CDS             complement(6089..6934)
                     /locus_tag="PLI76_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOI61721.1"
                     /translation="METMMMQKKLIWTLLALLCAGMLVSAASAAPTTELHVAKIASDG
                     VTVINETTVDYRWMEKNLPVLGDGVTHYYHQGPVFEGDKWDPLETTNFKGRGAVKGTN
                     IRDLAELVGGAAPGDEIMVRAADGYHVEFAYENVYNPDPRQGPIGICWYNGAEAGAGE
                     RQGCGYVPDYYTGMRLVFFADNSTNSEGLNVYGNQDMYDTLPESAQHFYDLYPSTGGL
                     TVKWVDEVRVYAGGFSGERGALAGSLTTPVAKATTEAAKAPLSLMVTLAAALGAVALH
                     ARREA"
     gene            complement(6922..8109)
                     /locus_tag="PLI76_06530"
     CDS             complement(6922..8109)
                     /locus_tag="PLI76_06530"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOI61722.1"
                     /translation="MRRYQKTRAAAVMLLALLLVPAASATIIWGPYVTNTTTGSATIA
                     WKATGEEPGWVEYSSEGGEVCRVASAGEEGMHRVHLAGLLPATTYRYRVGTGGATVED
                     CRFRTFGDRTFTCIIYGDTQGQRPFFTQTERHGLVAEKMAGEEDILFVIHTGDFVGDE
                     SEWDEFFALAGPVLGNTTLVPVAGNHDGTAEVFSAIFGLPPYYSFDAGSLHVTVLDSN
                     DRAWADMRTQTAWLKEDLTSPLPQTIVAFHHPPFSSDRKHPGGDLAIRAEWCGIFAQN
                     DVDAVFSGHTHTYERYRVNGTEYVVAGCGGGPFYPLSEEKPEGYQAGREETLGYIRAT
                     ISPVSIGIEVVPVADITRDGEVTMHPAGAVIDTVRLNTAGGWWSVLASAPGVWELPGL
                     WRR"
     gene            complement(8115..11006)
                     /locus_tag="PLI76_06535"
     CDS             complement(8115..11006)
                     /locus_tag="PLI76_06535"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOI61723.1"
                     /translation="MVSAHLRGSTPLLAALMFCLLLALAAPVTAGATTELHIKKLASD
                     GVSIIDEKTVDYRWLEANLPIQGDGRTHYYHQGPIFELAWEEKHPGEPYNFWNPEEDI
                     NILDKDMGAVKGTDLKDICELVGGMGEEDTVRVRATDGMSKMFPYRNVYEPESRQGPM
                     VITWYRTEDGYVPNYYTGMRLVFFADTSTNPYGIHAFGAWDMHECFDERYWYYFQPNL
                     PTTTGLSVQYVSDIIIYSEEEPVGSIYVNSTPPGATVYIDDEKAGNVTPCNIPGIEVG
                     TYPVRVELEGFAQSDEKWVTVRANTVTEVEFHLTPETGKIDVSSVPDDARIYLDGIDT
                     GKRTDTTLEDISVGKHTILLVKPDYRNATQTVTVEMDTTTKVRFDLTPENKSSGDDDS
                     SASGVENGSITITSVPANASIYLDGNKTGLFTDATLKDLPPGEHTIKLVLPGYLNMTR
                     TVTVEAQKRSFLDLIFENATPGKTVAGTGRVAVSSVPANASIYLDGNATGVRTNATLE
                     AVPAGRHTIGLMMPGYRNATKTIIVEESEHIALDLVLSPTGEEAGADYYDEPISGYTA
                     PEDTVEDPIRFPTGEKAGANTTARNEPTSGRTTPANPIEGFIGAVLGFLGNLFSIFGI
                     GGSTTPPPEPEITQVPVEHVTTPVPTDTPVPPVQEVPSVKNHSGGLYVDSYPPGMRIV
                     VDNEKLVRKTPGVIYGLREGLHTVTVEEGDPDRKTEDTGYRFEAVQVWVYLDTIAPAF
                     LDSLTTARKRTIRIDSNTCRGERFTVNGLFPAGTIPGNAGVESVRSWITVLWNGTYIS
                     YVVPYSVEEGGTFTVEPEEGGTVPVSVDSSPPGAAVFIDGFSTGMVTPCQVDGLSFGQ
                     HRIMISKVGYIPAEKVIRIPEGAGTGDTHTFALREYVSGDLLVESTVPDAKIYLYGRY
                     TGEKAPHTFSGMSIGTYKVRAVSENASRTVEDVLVTPGETTLCRVVLEE"
     gene            complement(11029..>11703)
                     /locus_tag="PLI76_06540"
     CDS             complement(11029..>11703)
                     /locus_tag="PLI76_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61724.1"
                     /translation="SDSGAGSESGGGRSPGDAGSQLAHAPAAPEAQTGRASLATSPAG
                     VVLESVTVRTVDEICAVAIREKTTARDAAGNPLNEVTVARTADVPAAPPGTTVAIALD
                     CGPAGATFDPPALVTYTLSAEEWAKIDRGATPKVMWYNPGSGAWQDIPATVNLATRTV
                     TVEVSHFSIYALVWTVPEKGFAGAEGTPAGSPVGETGPAFPVPALVLVVVLIAALAAF
                     LVMRKK"
BASE COUNT         1929 a         3739 c         3760 g         2275 t
ORIGIN      
        1 ccggttctcc aggtggcgct cgatctcctc gagctcgatc gtgcggtaga gatcgcggat
       61 gaagcggttc gtggcggtgc ggactggata gaggccggga cccccctgat caagagcgag
      121 ggcatgcggg ccgtgcggac gctccgggag cgcttccccg gccgcgagat cgtcgcggac
      181 atgaagatcg ccgataccgg ggccgtcgag gtggagatgg cggcaaagtc cggcgccggc
      241 atcgtctgca tccttgccga tgcggacgac accgtcgtcg ccgaggcggt ccggtcggcc
      301 cggaagtacg gcgtccggat catggccgac ctcatcaaca tcgacgaccc cgtcgcccgg
      361 tcgcgggagg ttgcggcgct cggcgtcgat tacatcaacg cccacgtcgg catcgaccag
      421 cagatgatag ggcggtcgtc gcttgacctc ctccgccgcc ttgccggcga ggtcgatatc
      481 cccatcgccg tcgcgggggg gctcgacgcg acgaccgcat ccggggcggt tgccgccggc
      541 gcctcgatcg tcatcgtcgg cggaaacatc atcaaggccg ccgacgtgac cgcggcggcg
      601 cagcgggtcc gggccgccat cgaccggccc gatatacgcc cgcgggagga agagagcccc
      661 gatgtcgcca tccgccggct ccttgaagcc gtctctgcgc cgaacgtcac cgacgccatg
      721 caccggaagg gagccatggc cggcgtcgtc tccctctgcg gcagggtgaa agcggtcggc
      781 cgtgcggtga cggtccggac cgtcgccggg gactgggcaa aacccgtcga ggcgatcgac
      841 gtcgccggac ccggcgacgt cctcgtcatc agtaacgacg ggggaaccca tatcgcgccc
      901 tggggtgaac ttgccaccca ctccgcgaag aaccgggggg ttagcggcgt cgtgatagac
      961 ggggcggtcc gggatgtgga cgacatccgg gagatggcat tcccggtctt tgcccgggcg
     1021 accgtcccga acgccggcga accgaagggt ctcggggaga tcaacgccga gatcgcctgc
     1081 tgcggtcagg aggtgcggcc gggagactgg atcgtcgccg acgagagcgg tgtcgtggtg
     1141 gtcccccggg agcgggccta cgaggtcgct cgccgggcga tggaggtcaa gaagaccgag
     1201 gagaggattc gcgaggagat ccggcgcgga aagacgctct cggaggtctc ggaactcctg
     1261 aagtgggaga aacggcactg agcgggggcc ggcgtcggtg cggccccccg tccccgcggt
     1321 agggttatgc tcttgtggtc accggaagag ggacgctttg aggagttctc cgccgtgatc
     1381 gaggatctgc cggacggcat cctcgacctt atccacgcgc aggataagga ccgccgcatc
     1441 cttccccgag taggcgtaag cgtactcgat gttgatcccc gcatccccga ggaccgatgc
     1501 gacctccgag aggccgccgg gttcgtcgcg catcttcacg ccgatgacgt cggtgaacga
     1561 tacccgaaat cctagccccg tcagcgtccg gtgagcatcc tccggccggt tgacgagcgc
     1621 cctgacgacg ccgaacccgt ttgcctcggc gatgctgaac gcaaatatgt tgattcccgc
     1681 atcccgcagc gcaccggcga ccgccgcaag gcgccccggg cggttctccg aaaagattga
     1741 gatctgtttg atgatgtatg atttctccat tacaatgacc tcctgtcgac aaccttcttt
     1801 gacttgccct caaagcgcgg gagggttccc ggctcgacca gttcgacgcc cacggcgacg
     1861 ttcaacgtgg tcttgaggcg gtgctccacc gccttcttga tcaccatgag ttcggtgatc
     1921 ttgtcggaga acgcctcctc gctcacctcc acccgcacga gcatggtatc gagcgcgccc
     1981 ttccggtcga tgacgatctg gaagtgcctg ccgacctcgg gtatctcgag cagcacgtgc
     2041 tccacctgtg aggggaagac gttgatgccc ctgacgatca gcatgtcgtc cacgcgcccc
     2101 tgtatccggc ggatccgcgg atgagtccgc ccgcaggcgc agggctcctc gtccaggacc
     2161 gtgatgtctc cgatccggta gcggaccagg gggaacgcct ccttctggag catggtgacg
     2221 accatctcgc cccgttcacc gggcgggagc gcctcgccgg tctcgggatc gacgacctcg
     2281 acgagggcga tgtcgcccca tatatgaatg ccctgctgct cggagcactc ggtgaacatg
     2341 ggcccggaga gttcgctcgt gccgtagatg tcgtaggcgc ggataccgag ccactcctca
     2401 agccggcccc gcatctgctc cgaccagggt tctgcgccga gtatgccgat ccggagatcg
     2461 gtgtcgttct tgatcgagac gcccatcttc tccgccacct cgcccatgtg gaggaggtag
     2521 gagggggtgc aggcgatggc ggtggtgtgg aggtcctgca tcagctcgat ctgccgttcg
     2581 gtgttcccga cgcttgtcgg gaggacggtt gcgccgaccc tctcggcgcc gtagtggacg
     2641 ccgaggccgc cggtgaagag gccgtagccg taactcacct ggagcacgtc cccgcgcccg
     2701 aggccgcagg aggagaacgc gcgcgccagc gactcgctcc agttctcgat gtcctgccgg
     2761 gtgtagccga cgacggtcgg cttcccggtc gtccccgatg agacgtggta gcggaccagc
     2821 tcatcctttt gtgcgcagaa gaggccgtca gggtagttat cgcggaggtc tcccttatac
     2881 atgaacggga gtttcccgat atccgcaagc gacccgatat cgtcgggccg aacatcgtgc
     2941 tccttcatcc gccgccggta gaactcgctc gtctcgtaga gccgccggat gagcgacttt
     3001 gcgagcctgt actggagctg ccgcagttcg tcggtcggca taacctcgat ctgccggttc
     3061 cagggcgcca tcagacggcc ccccggcggt cgatgaccct ctttgccttc ccctcgaacc
     3121 ggggcagcga cccgggctcc accagtttca ccgcggtccg gaggccgagg gtgctgtgga
     3181 gttcgccgac aatcttcttc tggacgcggg cgaggtcctg gagctcgccg gagaacccgg
     3241 cccgggtcat ctccacctcg atcgtcatct cgtcgaggtg ccgtacccgg tcgacgtaga
     3301 ccatgaactg gtccccgacc tccggaagag accggagcac gtgctcgatc tgtgacggga
     3361 agatgttgat cccccggata accagcatgt cgtcgctccg gcccgtgatc cgctcgatct
     3421 tctgtccgcg cccgcaggcg cactcgccct cgagcagccg ggtgatgtcg ccggtgcggt
     3481 agcggacgag cggcagggcc tctttgacga gcggcgtgat gaccatctct ccccgctcgc
     3541 cgggggcgag ccgttcgccg gtcacgggat cgatgacctc tgtaaggtaa cagtcgtgcc
     3601 agatatggag gccgttgtgc tccgggcact caaacgccac ccccgggccg tacatctccg
     3661 agagcccgta actgtcgtag gccttcaccc cgaggcgccg ttcgagctcc acccgcgtgt
     3721 tctccgacca gggttccgcc ccgaatatgc cggtcttgag tgtatcgagc gtctttccca
     3781 tcgactcggc cacttcggcg aggtgaaggg cgtaacttgg cgtgcagtgg atggcggtca
     3841 ccccgaagtc ctcgatcatc tcgatctggc ggcgggtgtt tcccgttgcg ctcgggatca
     3901 ccgtcatgcc gatcttctcg gcgccgtagt ggaacccgag accgccggtg aagaggccgt
     3961 agttgaccgc gttctgaaag acatcgtcta ccccgagacc gatcatcgtc atattccgcg
     4021 ctatcaggtc cgcccagttg tcgagatcct gccgggtgta gccgacgacg gtcggtttcc
     4081 ccgtcgtgcc ggatgtcgtg tgaatccgga cgatctctct catggggacc gcaaagaggc
     4141 cgaacgggta tcctgcctgg agctcctttt tgtaggtgaa cgggagcttc tccacatcgt
     4201 caagcgtcct gatatcgtcg ggcgatatgc ccgcttccct gaactttctt tggtatagcc
     4261 ctacttcctg cgcctggcga agcgcccatt tgagccgctt cagctggaga tccgcgagtt
     4321 cttcaggccg gattgtttct attgccctgt tccagaacat aatatcccat cggtatcgtg
     4381 ccggccgcat gctgcctggc atccgtcata cggttgcgat cctggcggcg gggagagccc
     4441 gcgccgccgg gcatacgcta cctttacatg ggtgtccggt gcggaaaagg tttgtcaatc
     4501 cgtagcgtgg tacactgtgg cagtccccgg atcctgccgc acggggtcgg gccatccggc
     4561 cctcccctcg cggaatgcat cgaggatgaa gtccacgaag atccgcgctt cggcggggtg
     4621 cggtgcgttt gccgggatgg tcatcgcgta gacgatcggc tgccctatcc gctcgctccc
     4681 gatggaccgg aaccgctgga acccgaggtt cacccgcacc ttccggtagt cgtcggcgtg
     4741 ctccggcgaa ccgaggttga ttgcgggggg aagatcgatc caccggagtc cgtgctcctc
     4801 cgcaacgctc ctgtactcga aggcgtagtc gatcccgccg gcatcgagga gcgagaggag
     4861 atagatgctc ccgtcacgga tggcgacctt ctcggccgcc ggcctcatca gttccggcag
     4921 ggcgatcgtc gtggcgccgt ccgtccagac cgccgcgatg ggcggcttga acgaaccgcc
     4981 tatgatggcc tcgaagagac cgggctcgcc gtaatactcc tctgcaagcg ccgtcaccat
     5041 gattgcccgg tagccgcagg catcaagcat ggggtttgag aaccctaccc gcacgtcggg
     5101 gcgggagagg atccggtacc agttctccgc cgtgatctcg cctgccccgg cgctccggcc
     5161 ggtatacgcg atcaccacct cgttctccgc aaagggaatg taccaatcgg tgtagtttcc
     5221 ctccccggca cccatcggca ggtacatcat atccgggatg agcgaggcgt cggcgaccag
     5281 gacaacgtcc acctctctcc cgaggtccgt cacctgccgg atggcctgga tgctcccgtg
     5341 cccttcgacc ctgacgtcga tatcggggtg ctggctctca aaggcggcct ctatatcctc
     5401 gagcgggaca aggaggctgc ctgccggtat caccatgagg atggtgcgct ccgggccggc
     5461 atcggcgcag cctgcggatg ccgcgagcac ggcaaggatg agcacggcga ggaccggaga
     5521 gcggttcatt tcctgtggac ctcgacccgg tcgacccatt tcacccaggg actcccttcg
     5581 gtgatgacgt cggggctctc ggttatgacg acgaggcgga gcggtccgcc gttctcgtag
     5641 gccagcggtt ctttatcctg ctcgtaggcc aggatcacgc ggagcggcgg cgacgtgacc
     5701 tcccggaggt tctcatcgaa ggtgaagaac ccctctccgt tcacctggtc catatcaaag
     5761 acccagagat acccgtcttc tgcagagacg tagatcaggt cgtccgcccc ggcgccgccc
     5821 gccatatcga tgagatcggc aagaagcaca ccgcggtagc ggttcgggcc ggtcttgatc
     5881 ccgaccgtcg agaccgagta gccgtagcct tcggcggccg ggagggccct gatctccggg
     5941 tgggtgagga cctgttcccg gtctccggca agcgtcaggt tccaggcagg atagtcgtcg
     6001 acggaaccgg tatcggtaca cccggccccg agggccaggg caccgatcag caccaggatg
     6061 agggggagag agatccggcg caccgggatc atgcctccct ccgggcatgg agcgccaccg
     6121 ccccgagtgc cgcggcgagc gtcaccatga gggagagcgg ggcttttgcc gcctcggtcg
     6181 tcgcttttgc caccggggtg gtgagggatc ccgcaagagc cccccgttcg ccggagaatc
     6241 cgccggcata gacccggacc tcgtccaccc acttcaccgt gaggccgccg gtggagggat
     6301 agaggtcgta gaagtgctgc gctgattcgg ggagagtatc atacatgtcc tggttcccgt
     6361 agacgttgag gccctcgctg ttggtcgagt tgtccgcaaa gaagacaaga cgcataccgg
     6421 tgtagtagtc agggacgtag ccgcacccct gccgctcgcc tgctcccgct tcggcgccgt
     6481 tgtaccagca gatccctatg gggccctggc gcgggtcggg gttgtagacg ttctcgtagg
     6541 caaactcgac gtggtagccg tccgccgccc ggaccatgat ctcgtcgccg ggtgcggcgc
     6601 cgccgaccag ttcggcgagg tcacgaatgt tcgttccttt cacggctcct ctgcccttga
     6661 agttcgtcgt ctccagggga tcccacttgt ctccttcaaa gaccggtccc tggtggtagt
     6721 agtgagtcac gccgtcgccg aggaccggga ggttcttctc catccagcgg taatcgaccg
     6781 tcgtctcgtt tatgacggtg accccgtcgg atgctatctt tgcaacgtgc agttcggtcg
     6841 tcggcgccgc cgatgcggcg cttaccagca tgcccgcgca gaggagcgcg agcagcgtcc
     6901 atatcagttt cttctgcatc atcatcgtct ccataatcct ggtaattccc atacgccggg
     6961 ggcggatgcg agcacgctcc accacccgcc ggccgtattg aggcggacgg tatcgatgac
     7021 ggcccctgcc ggatgcatcg tcacctctcc gtctctcgtg atatctgcga ccggcaccac
     7081 ctcgatgccg atcgataccg ggctgatggt tgcccggatg tagccgagcg tctcttcgcg
     7141 gcccgcctgg tagccttcgg gcttctcctc gctaagcggg tagaacggcc cgccgccgca
     7201 cccggcgaca acgtactccg tcccgttcac ccggtagcgc tcgtaggtgt gggtgtggcc
     7261 gctaaagacc gcgtcgacat cgttttgcgc gaatatgccg caccactcgg ccctgatggc
     7321 cagatcgccg ccggggtgtt tccggtcgga actgaacggc gggtggtgga acgccacgat
     7381 cgtctggggc agaggggatg tgaggtcttc ttttagccac gcggtctgtg tcctcatgtc
     7441 cgcccatgcc cggtcgttgc tgtcgaggac ggtcacgtgg aggctgccgg cgtcaaagga
     7501 gtagtagggc ggaaggccga atatagcgga gaagacctcc gccgttccgt cgtggttgcc
     7561 cgccaccggc accagggtcg tgttcccgag caccggccct gccagggcga agaactcgtc
     7621 ccactccgac tcgtcgccga caaagtcgcc ggtatggatg acaaagagga tatcctcctc
     7681 tccggccatc ttctccgcca cgaggccgtg ccgttcggtc tgggtgaaga agggtctctg
     7741 cccctgggtg tcgccgtata tgatgcaggt gaacgtccgg tccccgaacg tccggaacct
     7801 gcagtcctct accgttgcac cgccggtgcc gacccggtag cggtaggtgg tcgccgggag
     7861 gagcccggcg aggtgcacgc ggtgcatgcc ctcctccccg gcggaggcca cgcggcaaac
     7921 ttcgccgccc tcggaggagt actcgaccca tccgggctcc tcgccggtcg ccttccaggc
     7981 gatcgtcgcc gacccggtgg tggtgttcgt gacatacggt ccccatatga tggtcgccga
     8041 tgccgccggg acgaggagga gggcgaggag catgaccgcc gctgcccggg tcttctggta
     8101 acgcctcacc ctgttcactc ctcgaggact acccggcata gggtcgtctc tcctggcgtc
     8161 accagcacgt cctcgacggt cctcgaggcg ttctcggaga cggcccggac cttataggtg
     8221 ccgatgctca tcccggagaa ggtgtgcggc gccttctcgc ctgtgtaccg gccgtagaga
     8281 tagatcttcg cgtccgggac cgtgctctcg acgaggaggt cgccgctgac gtactcccgg
     8341 agcgcgaagg tgtgggtgtc gccggtgccg gccccttccg gtatcctgat aactttctcg
     8401 gcaggtatgt agccgacttt cgagatcatg atgcggtgct ggccgaatga gagcccatcc
     8461 acctggcacg gcgtaaccat cccggtggaa aacccgtcga tgaagaccgc ggcacccggg
     8521 gggcttgagt cgacgctcac ggggacggtg cctccctctt cgggctcgac cgtgaacgtc
     8581 ccgccctcct cgacgctgta gggaaccacg tatgagatgt aggtcccgtt ccagaggacg
     8641 gtgatccagg accttacgct ctcgaccccg gcattgcccg gtatggtgcc ggccgggaag
     8701 agcccgttca ccgtgaacct ctcgccacgg caggtgtttg aatcgattcg gatcgtcctt
     8761 ttgcgcgcgg tcgtcagact atcgaggaac gccggtgcga tcgtatcgag atagacccag
     8821 acctggacgg cctcaaaccg ataaccggta tcttctgtct tcctgtcagg atcgccctcc
     8881 tcgacggtga cggtgtgcag gccttcacgg aggccgtata tgaccccggg cgtcttccgc
     8941 accagcttct cgttatcaac gacgatcctc atgcccggtg gataggaatc gacgtagagc
     9001 ccgccggaat ggttcttcac gctcggcact tcctgtaccg ggggcaccgg ggtgtcggtc
     9061 ggcacgggag tcgttacatg ctcgacgggg acctgcgtga tctccggttc cggcggtggt
     9121 gttgtactcc cccctatccc gaagatcgag aagagattgc ccaggaaacc gaggacggcc
     9181 ccgatgaaac cctctatagg gttagccggc gtcgttcgcc cggatgtggg ttcattacgg
     9241 gccgtggtgt ttgctccggc cttctcgcct gtcgggaagc ggatcggatc ctctacggtg
     9301 tcttccggtg ccgtgtaccc ggatatgggt tcatcatagt agtctgctcc agcctcctcg
     9361 cctgtcgggg agaggaccag gtcaagggcg atatgctcgc tttcttcgac gatgatggtc
     9421 tttgtggcat tccggtaacc gggcatcatg agcccgatcg tatgtctgcc ggccgggacc
     9481 gcttccaggg tcgcattggt ccgtaccccc gtcgcgttcc catcaaggta gatgctcgcg
     9541 tttgccggca ccgaggatac ggcgaccctc ccggtcccgg cgacggtctt acctggagtg
     9601 gcgttctcga agatcagatc gaggaagctg cgcttctgtg cctcgacggt gacggttcgg
     9661 gtcatattca aatacccggg cagaacgagt ttgatcgtat gctcgccggg cggaagatcc
     9721 ttcagagttg catcggtgaa gagccctgtc ttattcccat cgaggtagat gctcgcgttc
     9781 gccgggaccg atgtgatggt gatgctcccg ttctcgacgc cactggcaga actatcgtcg
     9841 tcgccgctcg atttattctc gggagtcagg tcgaacctga ccttcgtggt agtatccatc
     9901 tcgacggtga cggtctgggt ggcgtttcgg tagtccggct tcacgagcag aatcgtatgc
     9961 ttgccgacgg agatatcctc aagggtcgtg tcggtccgtt tccctgtatc tatcccgtcg
    10021 aggtagatgc gtgcgtcgtc cgggaccgag gatacatcga tcttcccggt ctcgggggtg
    10081 aggtggaact cgacctccgt gacggtattc gctctgaccg tgacccactt ctcgtccgac
    10141 tgggcgaacc cttcaagctc gacccggacg ggataggtac cgacctcgat gccggggatg
    10201 ttgcaggggg tcacgttgcc ggctttctcg tcatcgatat agacggtagc cccgggaggg
    10261 gttgagttta cgtagatgct cccgacaggc tcttcctcac tgtaaatgat gatatcgctc
    10321 acgtactgca cggagaggcc cgtggtggtg gggaggttgg gctggaagta gtaccagtac
    10381 cgctcatcga agcactcgtg catatcccag gcgccgaagg cgtggatgcc gtaagggttg
    10441 gtcgatgtat ccgcgaagaa gacgaggcgc atcccggtgt aatagttggg gacgtacccg
    10501 tcttcagtcc ggtaccaggt gatgaccatc ggcccctggc gtgattccgg ttcgtagacg
    10561 ttccgatacg gaaacatctt tgacatccca tctgtggccc ggaccctgac ggtatcctcc
    10621 tcgcccatcc cgccgacaag ttcgcatata tctttgaggt ctgtcccctt caccgcgccc
    10681 atatccttat caaggatgtt gatatcctcc tcggggttcc agaaattgta gggctcgccg
    10741 ggatgctttt cttcccaggc caattcaaag atcggcccct ggtgatagta gtgggttctg
    10801 ccgtcaccct ggatcgggag gtttgcttca agccagcggt agtcgactgt cttttcatct
    10861 atgatgctaa ccccatccga tgccagcttt ttgatgtgca gctcggtcgt cgcgcctgcg
    10921 gtaaccgggg ctgccagcgc aaggagcagg cagaacatga gggcggcaag aagcggggtg
    10981 gagccccgca gatgcgctga taccatggtc catctcagag aagaggggtt atttcttcct
    11041 cataacgagg aatgctgcaa gcgcggcgat cagcacgaca acgagaacca gcgccgggac
    11101 cgggaacgca gggccggttt ctccgaccgg agaaccggcc ggggtcccct ctgctcctgc
    11161 gaaccccttc tccgggacgg tccataccag ggcgtatatg ctgaagtgcg agacttccac
    11221 agtgaccgtc cgcgtggcga ggttcaccgt ggcggggata tcctgccacg ccccggatcc
    11281 ggggttgtac cacatcacct tcggcgtggc acccctgtca atcttcgccc actcctccgc
    11341 agagagggtg taggtgacga gggccggcgg gtcgaacgtg gcgcccgcgg ggccgcagtc
    11401 aagggcgata gcaaccgtcg ttcccggcgg agctgccgga acatcggcag tgcgggccac
    11461 ggtcacctcg ttgagcgggt tgccggccgc gtcacgtgcg gtggtcttct cccgtatcgc
    11521 cacggcgcag atctcgtcga cggtcctgac cgtcacggac tcgagcacca caccggccgg
    11581 tgacgtggca agggatgcgc gcccggtctg tgcctcaggg gcagcggggg cgtgcgcaag
    11641 ctgcgacccc gcatctccgg gggatctccc tccgccactc tcgctaccgg cgccgctatc
    11701 act
//
LOCUS       DAOSEE010000068        11692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_2120_length_11692_cov_220.340638, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000068 DAOSEE010000000
VERSION     DAOSEE010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 11692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11692
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2120_length_11692_cov_220.340638"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..479
                     /locus_tag="PLI76_06545"
     CDS             165..479
                     /locus_tag="PLI76_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61725.1"
                     /translation="MNVIQGKQAGGWTFKVSHDVDYEKERKQVAAELMQFQKDHPELE
                     ILGCITSPTTIDMWVANLTPENEALNGTVMHGRTVLVNRSPVDYGLRMAREQASGEPG
                     TS"
     gene            618..1301
                     /locus_tag="PLI76_06550"
     CDS             618..1301
                     /locus_tag="PLI76_06550"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HOI61726.1"
                     /translation="MQEIASLRANPFFRQLRLLLFSPDRFFAERSTRPVDLKIPSLIV
                     CGLFLVEIVGNYDTIIAYARTLELGDPSAHPIVYVGLAATALSPFLAWVSYSAVFYGV
                     SYLYKGRGTFLRTLASVGYGCVPLIIGGLLYLVLFQFVSLPVQDYFVPARVAAHATAV
                     ALVDAGVAIFVLLWCGFLWTAGMRHARSLSTRQALVTVFVPVGLTIGAKTVLLLWKLV
                     SARVYTGVL"
     gene            1298..2500
                     /locus_tag="PLI76_06555"
     CDS             1298..2500
                     /locus_tag="PLI76_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HOI61727.1"
                     /translation="MSMTRSGMTLKTIGILLLLIACALPAAAASVVQLTNDSAFDAYP
                     FWSPDGSRIAYVSSDGVHAGIRVMGLDGQNPVPVTDDHSWNLFTEFDPWSPDGKKLLF
                     LSDDEGGLDLWTMNPDGTGRVRLTEGGRILPIPGLSGYGADWSADSGRIVYTSCLFDN
                     SAVWKSLVETTAEGSPAVNLSNVRKDADIWIMDADGGNKKQLTAGGDALLPLWQPHGD
                     RIAYLSDTSGKSEIWTVQSDGTGKTQVTFSEGDVSGYSWSPDGTRIACVVAAPPGTLP
                     EFSLWVIESDGSGSEQLTTGNRDRTPVWSPDGARIAFRSESRDRKLWVMESDGSGLAP
                     LDPDNPAYMMQQWSPDGRAIVVSDGNDLYAIRLEDPAAPGSPGFGVTAAAGALLLTAC
                     LFRLRKEE"
     gene            complement(2559..6239)
                     /gene="cobN"
                     /locus_tag="PLI76_06560"
     CDS             complement(2559..6239)
                     /gene="cobN"
                     /locus_tag="PLI76_06560"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HOI61728.1"
                     /translation="MKMTIVAWGSELLQFSDAAREAGIDLSAWQLHELRDEPEKRRYC
                     IEACRGSDVVLAHPSSDPVWNEILGGLAEGIPIIAFGYTDTWWSASTVPLSVVSTVNA
                     YFLYGGAENIQNLVRYVQAEVAGMDVAYDPPQEIHWEGAYHPDAERIFDDADAYLAWY
                     PSRHPIRVGLLLSRTRWANGDFTVEDTLIRSLEDYYDVLPVFCFGISDEEIGARSTRD
                     VVEMFFEGRVSALIDARAFTPPRDADAFTRTLEGLGVPVFHPLFLYHQTEAEWRDCID
                     GMRGSNVSWYVAMPEFLGGITMMPIGAAGSRNLAGIDSEQHIPMDERLERFVARVRRW
                     IDLAQKPAAGKRVAFILHNKPCASVEATVGAGAHLDTLESVARILEAMHDAGYAVECP
                     ESGKTLIDDIMNRKAISDFRWTSVTEIVRKGGALALVEPEEYAAWFETLPPAVRTRIV
                     ETWGQPPGEAMVYEGKIVVTGVRYGDAIVCVQPKRGCAGSQCDGEVCKILHDPDIPPT
                     HQYLATYRYIEEVFGADAIIHVGTHGNLEFLPGKSVALSDACYPDIAIGTVPHLYIYN
                     ADNPPEGTIAKRRSYAVLVDHMQAVMTSSDLYAGLKELEEQIADYNRARGVDPARAHA
                     LEHTIADLLEETGIADEIGFRGLHEDNRPFEDVIAAAHTSVTRIADTRIPDGMHVFGE
                     IPEGERLAEFIDAIMRYGGEAKGAVLRMMGHNVSITDEDLLRDADSRAKDLIAAAVAE
                     EPLDRAAERILAGSLQNLDLAALEEIRAIVLDLTARIEDSDEIGSLLSGCAGSYIPPG
                     PSGLITRGKPEVLPTGRNFYSLDPFRIPTRAAWRIGMRLAESIIGKYVEEQGRIPENV
                     GMYWMASDVMWADGEQLAQIFHLLGVEPVWIDGRVRSFRIIPLEELGRPRIDVTVRMS
                     GILRDCFYTCIELLDDAISEVAALDEPPDMNLVRKHALQGLGTTRIFGSKPGTYGNGV
                     SLAVYASAWREEADLAEVYLRWNSYAYGRGNFGEEAREAFSAHLATIDLTFNKTVTDE
                     YDLLGCCCYFGGHGGLTGAARVLSNRDIPAYYGDTRDRDRVEVRTLAEEVRRVVRAKL
                     LNPKWIEGMKRHGYKGAGDISRQVGTVYGWEATTEEVDDRIFDDIVRTFVLDAKMRQF
                     FADENPWALEEIGRRLLEAHGRGLWDADPDVLEGLRAAYLEIEGWIEDGMDDAGGGVQ
                     GGAIRVVTVEDAEALRGARKRE"
     gene            complement(6236..7030)
                     /locus_tag="PLI76_06565"
     CDS             complement(6236..7030)
                     /locus_tag="PLI76_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI61729.1"
                     /translation="MPLLCARGVSFAYNERRVLEDISFTLDAGEVLGLVGPNGSGKTT
                     LIRCLDRILAPEGEIHLDGRDMLSMSRPEIARMIAYVPQNSGILSSATVFETVLMGRR
                     PHLRWSVSEEDKQTVSDALSLLGISAFASRRIDRLSGGERQRVFIARAIAQDARVLLL
                     DEPTSALDVRNRLEVMALLFRLAEERGLAVVAAIHDLNLAARYCHRLLILRDGLVLGQ
                     GTPSSLLTPGMVRAAYGIEAAVKDEVGVPYVVPLRPSDNDRPVELP"
     gene            complement(7030..8094)
                     /locus_tag="PLI76_06570"
     CDS             complement(7030..8094)
                     /locus_tag="PLI76_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOI61730.1"
                     /translation="MMAETGKQEYTRLTEKRVLFIVGLILILIALAGVAVTLGSANLS
                     VADSYLAILAGLFPDAVDLPDRMIESNAVGIVWGWRLHRVLFGIAAGFGLAIAGTVMQ
                     GVLRNPLASPFTLGIASAASCGASIAIVLGAGILSGSLLVIGNAFLFAMLAAAAIYGM
                     ARLRGMGSEAMILAGIALMYLFSAITSLLQYLGTAAKVQEVVFWMFGSLDKSSWPKLG
                     IVAIVIVAVIPPLLWRAWDLNALAEGDEVAASLGVPVERSMAGFMLAASLVTAVIICF
                     TGTIGFIGLVAPHITRMAIGTDHRTLLPASGLVGAVLLLGADCLARTLIPGAIIPVGI
                     MTAFLGIPFFLYLFMRGRDA"
     gene            complement(8091..9305)
                     /locus_tag="PLI76_06575"
     CDS             complement(8091..9305)
                     /locus_tag="PLI76_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOI61731.1"
                     /translation="MTRRLILLACSLCLLAGMAMAIPCDRDSDGTLTSSELATAILDS
                     LDVRQMGGAVEAPSPGDLRDAAFIYEHWDGRALTITDTSGRTATLTRPIRRIALFNSD
                     SLEMLRSIGIEPDRIVGVSKYVLEDPIFYPEYQGKANLGSVWSPDYEQAAGIRPDAVF
                     LYASISQSSCDEIEDTLSAIDPDIRFFRFDSFRPTVYADEVRTLGLLLGKREGAGRFL
                     AFYENATGSVAGVVDTIPVKDRIPVYLENWNDYKSAGKGSGYDEKIELAGGMNIFGDN
                     PVEYPVIDPEAVISKNPGVIVKIVGAGETTFGGYGDDDISPFERVHRAISDRPVWNQM
                     KAIRDGRVHIIHTDVIGGPEYFIGTAYFAKWFYPDRFPDLDPRAVHRQYLEEFQRLDY
                     DLDEHGTFVYPA"
     gene            complement(9302..9769)
                     /locus_tag="PLI76_06580"
     CDS             complement(9302..9769)
                     /locus_tag="PLI76_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61732.1"
                     /translation="MTPRRSVTLCILIVLLAAPVTAANIERIVTSSGDGLDVTLVVTG
                     IPVGGIVETLPDGCVWKETDHPADRTRLSGGKAAFAVIGEETIRYRVQGPQEAAGVFT
                     GTWEDLLTGESGTVGAAGTHGPESSKTPVEAPGFLSVAALAALAVAYRRRCGR"
     gene            10330..>11692
                     /locus_tag="PLI76_06585"
     CDS             10330..>11692
                     /locus_tag="PLI76_06585"
                     /inference="COORDINATES: protein motif:HMM:NF023252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3344 domain-containing protein"
                     /protein_id="HOI61733.1"
                     /translation="MTVPVSAIYDFEGIPLDTAAQGEVRGDVLTFGTYGLAAPPIECA
                     FSLPAKPVWARVYTGVWGGTERYTGWEELSVNNGVPVRRDLFGKDDRHPETLVSGYGV
                     YWVAHDCTDQLRGGKNTVTLTTSRGQPESRIDGRVYGIFVVAVVEDAARPVTRYWIAE
                     GNENLHGEGWSGTNPTRHDTCEVVFEKAGAIEKGAADLTVVLLASTKGQPDYVLFNGE
                     DLGVPVTDLSIYPEGARDIGNEHIGDATGGAGVEARYVDVETFDVGRLLKETNRIVFE
                     QGRDLDGDGEISTTGSKPEGEDYIHPVSAILTVQRPGASPAPDFAVDTPVVTGAYAGE
                     EATIRAMVRNYGARPDGKIAVAFSVDGSAVKTVEVEPARSGVQQVETGVTLAEGRHTV
                     AVRVDAAGDADPADNEASMQLTVGTVPDLSVAIGAPVRAGAGAHAAPTQQAPAPFFAL
                     FAGL"
BASE COUNT         2222 a         3645 c         3682 g         2143 t
ORIGIN      
        1 cgatctggcg atcatcgggg aatgcagcaa cagatcctta aggttgccca ttaaaagatc
       61 tacgacgcgg tgagaacaga acattcagca tcagtaaatg ggaatttgac ccggacaaca
      121 aactggtcgt catctatgcg tactccatat gggacgaaaa aggggtgaat gtcattcagg
      181 gcaaacaggc cggcggctgg acctttaagg tcagccatga tgtggattac gagaaagagc
      241 gtaagcaggt tgcggccgaa ctgatgcaat tccagaagga ccatccggaa ttggaaatat
      301 tgggctgtat tacatcaccc acaactatcg atatgtgggt ggccaacctc acacccgaaa
      361 acgaggcact gaacggcacc gtgatgcacg gcaggacggt tctggttaac aggagccctg
      421 ttgattatgg actcaggatg gcgagagagc aggcgtcggg ggaaccgggg acaagttgag
      481 gcacagagac tctatccggg gacatgagtt tcctccgcct ctcctccccc agcgcactac
      541 ggacccggtg acctgtaagt gcaggatttg cctttaaata ctcctaaacc atgaaaatga
      601 gtgattatat gtcccctttg caagaaatcg cttccctccg ggcaaaccca ttcttccgac
      661 agttgagact gctgctcttc tcacccgatc ggttttttgc agaacgttcg accagacccg
      721 tcgacctgaa gattccatcc ctgatcgtct gcggcctatt cctggttgag atcgtcggca
      781 attacgatac aatcatcgcg tacgcccgaa cgctggagct cggcgacccg tccgcgcacc
      841 cgatcgtata cgtgggcctg gctgcaacgg cgctctcacc gttcctggca tgggtatcgt
      901 actcggcagt cttttacggg gtatcgtacc tgtacaaagg ccggggtacg tttctccgga
      961 cgctggcttc tgtgggatac gggtgcgtgc ccctgatcat cgggggcctc ctctacctgg
     1021 ttctctttca gttcgtctcc ctgccggtgc aggactattt tgttcccgct cgtgtggcgg
     1081 cgcatgcaac cgccgtagcg ctggtcgatg caggggttgc gatttttgtt ctcctctggt
     1141 gcgggttcct ctggaccgca ggtatgcggc acgcacgaag cctctccacc cgtcaggcac
     1201 tggtgacggt cttcgttccc gtggggctga cgatcggtgc aaaaaccgta ttgcttctgt
     1261 ggaaactggt atcagccaga gtgtatacag gagtattatg agtatgacaa gatccggtat
     1321 gaccctgaaa acgataggta tcctcctgct gctgatcgcg tgcgctctcc cggcagcggc
     1381 cgcatccgtc gttcaactga caaacgattc ggcattcgat gcgtatccct tctggtcgcc
     1441 ggacggttcc aggatcgcct acgtctcatc cgacggagtt catgccggca tccgggtgat
     1501 ggggctcgac ggccagaacc cggtaccggt gacggacgac cattcgtgga atctcttcac
     1561 cgagttcgat ccctggtcgc cggacgggaa gaaactcctc ttcctctccg acgacgaagg
     1621 agggctcgac ctctggacga tgaaccctga cgggaccggg agggtgcgcc tgaccgaagg
     1681 cggccgcatc ctccccattc cggggctctc cggctacggg gcggactgga gcgccgatag
     1741 cggacggatc gtctatacct cctgcctctt cgacaattcg gctgtctgga agagcctcgt
     1801 ggagaccacc gcagaagggt cgcctgcggt gaacctctcg aatgtccgga aggatgccga
     1861 tatctggatc atggacgcgg acggcggcaa caagaagcag ctgacggccg gcggagatgc
     1921 acttctgccc ctgtggcagc cgcacgggga caggatcgca tacctctcgg atacctccgg
     1981 gaagagcgag atctggaccg tgcagagcga cggaacgggc aagacacagg tgacgttcag
     2041 cgaaggggac gtatccgggt attcctggtc cccggacggc acccggatcg cctgcgtcgt
     2101 cgcggcaccg ccggggacat tgccggagtt ctctctctgg gttatcgaga gcgacggatc
     2161 gggttcggag caattgacga ccggaaaccg ggacagaaca ccggtctggt cgccggacgg
     2221 tgccaggatc gccttccggt cggagtcgcg ggaccggaag ctctgggtga tggagagcga
     2281 cggctccggc cttgcacccc tcgatcctga caacccggca tacatgatgc agcagtggtc
     2341 ccccgacggc agggcaatcg tcgtgagcga cggaaacgac ctgtatgcaa tccggctcga
     2401 agatccggcg gcacccggat cgccgggatt tggggtaact gctgcagcgg gtgcgctgct
     2461 tctcactgca tgtctgttca ggctgcgaaa agaggaatga ggggaaaagc ggggatgtgc
     2521 cgcatgatgg agtgcgatgc gttaatgggt atcgccgctc actctctctt ccgtgcccct
     2581 cgcaacgcct ctgcatcctc tacggtcacc acgcggatgg caccgccctg gaccccgccg
     2641 ccggcatcgt ccatcccgtc ctcgatccag ccctcgatct ccaggtaggc cgcccgcagc
     2701 ccctcgagga cgtccgggtc ggcgtcccag aggccgcgtc cgtgcgcctc gagcagccgc
     2761 ctcccgatct cctcgagcgc ccagggattc tcgtccgcga agaactgccg catctttgca
     2821 tccaggacga acgtgcggac gatgtcgtcg aagatccgat catccacctc ctcggtcgtt
     2881 gcctcccacc cgtagacagt ccccacctgc cgcgagatat ccccggcgcc cttgtagccg
     2941 tgccgcttca tcccctcgat ccatttcggg ttgaggagtt tcgccctgac gacccgccgc
     3001 acctcttcgg caagcgtcct gacctccacg cggtcccggt cgcgggtatc gccgtagtag
     3061 gcggggatgt cccggttcga gagcaccctt gccgccccgg tcagaccgcc gtgccccccg
     3121 aagtaacagc agcacccgag aaggtcgtac tcgtcggtca ccgtcttatt gaacgtgagg
     3181 tcgatggttg caaggtgtgc ggagaaggcc tcccgggctt cctcgccgaa attgccccga
     3241 ccgtaggcat agctgttcca ccggagatag acctccgcga gatctgcctc ctccctccag
     3301 gccgaagcat agaccgccag gctgacgccg ttgccgtaag tgcccggctt gctcccgaat
     3361 atccgggtcg tccccagacc ctggagagcg tgtttccgta ctaagttcat atcggggggc
     3421 tcatccagcg ccgcgacctc agagatggca tcatccagga gctcgatgca ggtgtagaag
     3481 cagtcccgga ggatcccgct catcctgacg gtgacgtcga tcctcggtcg cccgagttcc
     3541 tcgagcggga ttatgcgaaa cgatcgaacc ctcccgtcaa tccagaccgg ctcgaccccg
     3601 aggaggtgga agatctgtgc gagctgctcg ccgtcggccc acatgacgtc tgacgccatc
     3661 cagtacatcc cgacattttc cgggatccgc ccctgctcct cgacgtactt tcctatgatg
     3721 ctctcggcaa gccgcatccc gatccgccat gcggcgcggg tggggatgcg gaaggggtcg
     3781 agcgagtaga agttccgtcc ggtggggaga acctcgggtt ttccgcgggt gatcaatccg
     3841 gaggggccgg gcgggatgta gcttcccgca cagcccgaga gcaggctccc gatctcgtcc
     3901 gaatcctcga tccgggctgt aagatcgagg acgatggccc ggatctcctc aagtgccgca
     3961 agatcgagat tctggagaga gccggcgaga atccgttccg ccgcccggtc caggggctcc
     4021 tctgcgacgg ctgcggcgat gagatctttc gccctggaat cggcgtcccg gagcagatcc
     4081 tcgtcggtga tcgagacgtt atgccccatc attcggagca ccgcgccctt cgcctcgccg
     4141 ccataccgca tgatggcatc gatgaactcc gccaggcgtt caccctccgg tatctcccca
     4201 aagacgtgca tcccatcagg gatccgggta tcggctatcc tggttacgga ggtatgggct
     4261 gcagcgatta catcctcaaa cggtctatta tcttcgtgga gaccgcgaaa cccgatctca
     4321 tcagctatac cggtctcctc gaggaggtcg gcgatggtgt gctcaagcgc atgcgccctc
     4381 gccgggtcga cgcccctcgc ccggttgtag tcagcgatct gctcttcaag ttccttcagg
     4441 ccggcataga ggtcgcttga ggtcatcacc gcctgcatgt gatcgaccag gaccgcgtag
     4501 ctccgccgtt tggcaatcgt cccctcaggc gggttgtcgg cgttgtagat gtagaggtgg
     4561 gggacggtcc cgatggcgat atcggggtaa caggcgtcag agagggcgac cgacttgccg
     4621 gggaggaact cgaggttccc gtgcgtgccg acatggatga tcgcatccgc accaaacacc
     4681 tcctcgatgt agcggtaagt cgcgaggtac tggtgggtcg gcgggatatc cgggtcatgg
     4741 aggatcttgc agacctcgcc gtcgcactgt gatccggcgc acccgcgctt cggctggacg
     4801 cagacgatag catctccata gcgaacgccg gtgacgacga tctttccctc gtagaccatc
     4861 gcttcacccg gcggttgccc ccacgtctcg acgatgcggg ttctgactgc aggggggagg
     4921 gtctcaaacc aggcggcgta ctcctccggc tccaccaggg caagagcgcc ccccttccgg
     4981 acgatctcgg taaccgatgt ccagcggaaa tcgctgattg cttttctgtt catgatgtca
     5041 tcgatcagcg tcttcccgct ctccgggcac tcgaccgcat accccgcatc atgcatcgcc
     5101 tcaaggatcc gggcgacgct ctccagcgta tcgaggtgcg ccccggcccc gaccgtcgcc
     5161 tcgaccgatg cgcagggctt gttgtggagg atgaaggcga cccgtttccc ggcggccggc
     5221 ttctgcgcaa ggtcgatcca cctcctgacc cgggccacga acctctccag ccgctcatcc
     5281 atggggatgt gctgctcgct gtcgatgcct gccaggttcc ggctcccggc cgcaccgatc
     5341 ggcatcatgg tgatcccgcc caggaactcg ggcatcgcga cgtaccatga gacgttgctc
     5401 ccgcgcatac cgtctataca gtccctccat tcggcctcgg tctggtggta gaggaacagg
     5461 ggatggaaga ccggcacgcc gagcccttca agcgttcttg tgaaagcatc ggcgtcgcgg
     5521 ggcggcgtga atgcccgcgc atcgatgagc gccgagaccc gaccctcaaa gaacatctcc
     5581 accacatccc gtgtggaccg cgccccgatc tcctcgtcgg agattccgaa acagaagacc
     5641 gggaggacat cgtagtagtc ctcgaggctt cggatcaggg tatcctcaac ggtaaaatcc
     5701 ccgttcgccc accgggtccg ggagaggagg agtccgacgc ggatggggtg ccgggaggga
     5761 taccaggcga ggtatgcgtc agcatcgtcg aagatgcgct ccgcatccgg gtggtaggcc
     5821 ccctcccagt ggatctcctg cggagggtcg tacgcaacgt ccataccggc gacctctgcc
     5881 tgaacgtagc ggaccaggtt ctggatattc tcagcccctc catagaggaa gtaggcgttc
     5941 acggtcgaga cgacactgag cgggacggtc gaggccgacc accaggtatc agtgtagcca
     6001 aacgctatga tgggaatccc ttccgcaagg ccgccgagga tctcattcca gaccgggtcg
     6061 ctcgagggat gggccagaac gacgtccgaa ccccggcacg cctcgataca gtatcttcgc
     6121 ttttccggtt cgtctctcaa ctcatgcaac tgccacgccg agagatcgat ccctgcctcc
     6181 cgcgccgcat cggagaactg caacagttcg ctgccccagg caactatagt catcttcatg
     6241 gtaactccac aggtctgtcg ttgtcagaag gtcggagggg gaccacgtag ggcaccccca
     6301 cctcgtcctt tacggccgcc tcgatgccgt aggccgcgcg caccattccc ggcgtcagga
     6361 gggaagacgg ggtcccctgc ccgagcacca gcccatctct cagtatcagg agccggtggc
     6421 agtagcgtgc ggcaaggttc aggtcgtgga tcgccgccac caccgcaagc ccgcgctcct
     6481 cggcgagccg gaagaggagg gccatcacct caagccggtt gcgtacatca agcgcgctcg
     6541 tcggctcgtc gaggaggagc acccgggcgt cctgcgcgat cgcccgcgcg atgaacaccc
     6601 gctgccgctc cccgcccgag agccggtcga tcctgcggga cgcaaacgca gatatgccga
     6661 ggagggagag cgcgtcagag accgtctgct tatcctcttc ggagacgctc cagcgcaggt
     6721 gcggccgcct gcccatcagg acggtctcaa agaccgtcgc cgaactgaga atgccgctgt
     6781 tctgtgggac gtaagcgatc atccgcgcga tctccggccg gctcatcgag agcatgtcgc
     6841 gcccgtcgag atggatctcc ccctcaggag cgaggatccg gtccaggcac cggatcagcg
     6901 tcgtcttccc cgatccgttc ggcccgacga gcccgaggac ctcgcccgca tcgagcgtga
     6961 acgatatatc ctcgagcacc cggcgctcgt tgtaggcgaa cgagaccccg cgcgcgcaga
     7021 gcagcggcat caggcatccc tccccctcat gaagaggtag aggaagaagg gtatgccgag
     7081 gaacgcggtc atgataccga ccgggatgat ggccccgggg atcagggtgc gggcgaggca
     7141 gtcggcgccg aggaggagaa cggcccccac gagaccggat gcagggagta gggtgcgatg
     7201 atcggtcccg atcgccatcc gggtgatgtg gggcgccacg aggccgatga accctatggt
     7261 gccggtgaag cagatgatca cggccgtcac gagcgatgcg gcgagcatga acccggccat
     7321 ggaccgctcc accgggacgc cgagactggc tgccacctcg tcaccctcgg cgagggcatt
     7381 gagatcccac gcacgccaga ggaggggtgg tatgacggcg acgatgacga tcgccacgat
     7441 cccgagtttc ggccacgacg atttgtcgag cgatccaaac atccagaaga cgacctcctg
     7501 caccttggcc gccgtcccga ggtactggag gagggatgtg atcgccgaga agaggtacat
     7561 gagtgcgatc ccggcgagga tcatagcctc gctccccatg ccccgcaggc gggccatgcc
     7621 gtagatggcg gccgcggcga gcatggcaaa gaggaaggcg ttgccgatga cgagaaggct
     7681 gccggagagg atgccggcgc cgaggacgat cgcgatcgat gcgccgcatg acgcggcgga
     7741 cgctatgccg agggtgaagg ggcttgcgag cgggtttcgg aggacgccct gcatcaccgt
     7801 gccggctata gcaagcccga accccgccgc gataccgaag aggacacggt gcagccgcca
     7861 gccccacacg atgccgacgg cgtttgactc gatcatccgg tcaggcaggt cgaccgcatc
     7921 gggaaagagc ccggcgagga tggcgaggta ggagtcggcg accgagaggt tggccgatcc
     7981 aagggtcacg gcgaccccgg cgagcgctat gaggatcagg atgaggccca cgatgaagag
     8041 cacccgcttc tcggtgagcc gcgtgtattc ctgtttgccg gtctcggcca tcatgccggg
     8101 tacacgaatg ttccgtgttc atcgaggtcg tagtcgagcc gctggaactc ctccaggtac
     8161 tgccggtgga cggcgcgggg gtcgaggtcg gggaaacggt cggggtagaa ccacttcgca
     8221 aagtatgcgg tgccgatgaa gtactcaggc cccccgatga catcggtgtg gatgatatgc
     8281 accctgccgt ccctgatcgc tttcatctgg ttccagacgg ggcgatcgga gatcgcgcgg
     8341 tgaacccgtt cgaagggcga gatgtcgtcg tcgccgtagc cgccgaacgt cgtctccccg
     8401 gcgcccacga tcttgacgat gacgccgggg tttttgctga tgaccgcctc gggatcgatc
     8461 accggatact cgacggggtt gtcgccgaag atgttcatgc cgccggcaag ttcgatcttc
     8521 tcgtcgtagc ccgagccctt ccctgcgctc ttgtagtcgt tccagttctc aaggtagacc
     8581 gggatccggt cttttaccgg gatcgtatcg accacccccg ccacgctgcc ggtggcgttc
     8641 tcgtagaacg cgaggaaccg ccccgcaccc tctctcttcc cgaggaggag gccgagcgtc
     8701 cgcacctcat cggcgtagac cgtcggccgg aagctgtcga accggaagaa gcgtatgtcg
     8761 gggtcgatgg cgctgagggt atcttcgatc tcgtcgcagg acgattggga gatggaggcg
     8821 tagaggaaga ccgcatcggg gcggatgccg gccgcctgct cgtagtcagg ggaccagacc
     8881 gaaccgagat tcgctttgcc ctggtattcg ggatagaaga tcgggtcctc gaggacgtac
     8941 ttgctcacgc ccacgattcg gtcgggttcg atgcctatcg accgcagcat ctcgagagaa
     9001 tcgctgttga agagcgcgat cctcctgatc gggcgggtga gcgtcgccgt ccgtcccgat
     9061 gtatcggtta tcgtgagtgc ccggccgtcc cagtgctcgt agatgaacgc ggcgtcccgg
     9121 aggtcgccgg gcgacggtgc ttcgactgct ccgcccatct gccggacatc cagcgagtcg
     9181 aggattgctg tcgcaagctc ggacgacgtc agcgtgccgt cgctgtcgcg gtcgcacggg
     9241 atcgccatcg ccatccctgc gaggaggcag agagagcatg cgagcaggat gaggcgcctg
     9301 gtcatctgcc gcacctccgg cggtatgcaa cggcaagcgc ggcgagtgcg gccacggaaa
     9361 ggaagccagg ggcctccacc ggagttttgc tggactcggg gccatgggtt ccggcggccc
     9421 cgacggtgcc tgactcgccg gtcaacagat cttcccaggt gccggtgaag actccagcgg
     9481 cctcctgtgg tccctggact cgatagcgta tcgtttcttc gccgatgacg gcgaaagcag
     9541 cttttccgcc cgagagccgg gtgcggtcag cgggatgatc cgtctctttc catacgcatc
     9601 catcggggag cgtctcgacg atgcccccga ccgggatgcc ggttaccacg agggttacat
     9661 caaggccgtc tcccgaggat gtgacgatcc gctcgatgtt cgcagccgtt acgggggccg
     9721 cgagcagaac gatcaggata cacagggtta ctgatctcct cggtgtcatt agagagggat
     9781 tcatcacagc gtgttaaata tattgtcatt tgtatcattt attcgaagtt attttaaaac
     9841 acggcctatg gaatgggaat aaacacgaaa tacatattaa catccatggg gctctcccgg
     9901 agcatcgatg aaggcctgct cttgtcggga aggacaaacg cattccaccc gccggggctc
     9961 atgcaccccg gcaggcgcgc ttccgatctg caggagtttc aggccttcga ggaagttttc
    10021 ggcagcggtg cgcaggggca atgtgccgga ggaccggaac ctgcaggtag tcctatccgg
    10081 ataatcgagc aaatcaggct ggggcacaag tagaaaatga tctggctgtt tataagggtc
    10141 tgatggaaat aactccaatt tgaggttctt ttgcggcacc tgcggggttg tgtcccagcc
    10201 tgaaatgaga gcacacgata tcgcaaaaaa ataaaaggta ttaatacatg gtgctacctt
    10261 atgacaaatt atatgaatat attcagaaat atccacatat ggttatgcgt gatcatggca
    10321 tctatcttca tgacggtgcc ggtttcggca atctacgact tcgagggcat ccccctcgat
    10381 acagcggccc agggcgaggt cagaggggac gtgctcacct tcgggactta cgggctcgcc
    10441 gctcccccta tcgagtgcgc cttctccctc ccggcaaagc cggtctgggc gcgggtctac
    10501 acgggtgtct ggggcggcac cgagcgctac accgggtggg aggagctcag cgtcaacaac
    10561 ggcgtcccgg tccgccggga cctcttcggg aaggacgacc ggcaccccga gacgctcgta
    10621 tcgggatacg gcgtctactg ggtcgcgcac gactgcaccg accagctccg gggcggcaag
    10681 aacaccgtca ccctcacgac cagcaggggc cagcccgaga gcaggatcga cgggcgcgtc
    10741 tacggcatat tcgtcgtggc cgtcgtcgag gatgccgcaa gaccggtcac ccggtactgg
    10801 atcgcagaag ggaacgagaa cctccacggc gagggatggt ccggcacgaa ccccacgcgc
    10861 cacgatacgt gcgaagtagt cttcgagaag gcaggcgcaa tagagaaggg cgccgccgac
    10921 ctgaccgtgg tccttctcgc ctcgacgaaa gggcagccgg attacgtcct cttcaacggc
    10981 gaggacctgg gcgtcccggt gaccgacctc tcgatctatc ccgaaggtgc gcgggacatc
    11041 ggcaacgaac atatcggcga cgcaaccggc ggtgctggtg tcgaggcccg ctacgtcgac
    11101 gtcgagacgt tcgacgtcgg ccgcctcctg aaagagacca accgcatcgt cttcgagcag
    11161 gggagggacc tcgacggcga cggcgagatc agcaccaccg ggtcgaagcc cgagggcgag
    11221 gattacatcc acccggtctc cgcaatcctc acggtgcaac ggcccggcgc gtctccggcg
    11281 ccggacttcg ccgtcgatac acccgtggtg acgggcgctt acgccggcga ggaggcgacg
    11341 atccgggcga tggtgaggaa ctacggggcg cggcccgacg ggaagatcgc ggtggccttc
    11401 tcggtcgacg ggagcgccgt caaaacggtt gaggtcgagc cggcgaggtc cggcgtccag
    11461 caggtcgaga ccggcgtcac cctcgccgaa ggaaggcata ccgtcgcggt gcgcgtcgat
    11521 gccgccggcg acgccgatcc cgcggacaac gaggcgtcga tgcagctgac ggtcggcacg
    11581 gtccccgacc tctccgtcgc cataggggca ccggtccggg ccggggctgg agcgcacgcc
    11641 gcgccgacgc aacaggcgcc cgctccgttc tttgccctgt ttgcggggct cg
//
LOCUS       DAOSEE010000069        11362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_2190_length_11362_cov_251.662598, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000069 DAOSEE010000000
VERSION     DAOSEE010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 11362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11362
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2190_length_11362_cov_251.662598"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..801
                     /locus_tag="PLI76_06590"
     CDS             247..801
                     /locus_tag="PLI76_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TATA-box-binding protein"
                     /protein_id="HOI61734.1"
                     /translation="MDEKGYESLKIENIVASGVIADSIDLEKVSNKIKNCELNTKRFP
                     GAVYRIEKPKIASLIFSSGKVVLTGIRNKEDLHAGLDIIMHSLRDAGIDTYDEPQVAV
                     TNIVCSYDMGRYINLNKVVITLNLENIEYEPEQFPGLVYRIEDPKIVALLFSSGKIIL
                     TGGKNIEDIKRGLDFLEQRLETIM"
     gene            complement(1118..3847)
                     /locus_tag="PLI76_06595"
     CDS             complement(1118..3847)
                     /locus_tag="PLI76_06595"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOI61735.1"
                     /translation="MVRTSAPRPRSAPPAAGELLSPAVREVVRKRGFTALSDAQEQAI
                     PLLLEGKNLVLVAPTGTGKTESAMLPVFDRLLATAGPGFKAVYVTPLRSLNRDILARM
                     EWWCRELSLSAGVRHGDTPQNERRKQALNPPDLLITTPESLQALFMGKRLREHLKNVR
                     YVIIDEIHELADSKRGAQLAVALERLAAYAGEFQRIGLSATVGNPDDIGRFLCGARAF
                     SLVEVPVASHLEIGVRFAGEDFAAQTRAVGDCLDAPGSTLVFVNTRVTAEALGHELYP
                     RGDVEVHHGSLSRDVRVEAEERFREGAVRTLIATSSMELGIDIGHIEHVVQFGSPRQV
                     SRLVQRVGRAGHRLDAISRGTVLATGFDDLLESLVIARRAKANECERIVPPAGAADVL
                     ANQVAAIAVEYGEIEVSRIRDMIERSGVFAGCGPLLDTVCSQMAEHRLIRLDGTRVTR
                     TGRARRYLIDNLSMIPDERKVEIFDMVSRRTVGTLDESFVLGWMHTGAIFITKGQLWQ
                     VLDIEGGRITVEPAARARGEVPTWEGEQIPVPFAVAREVGALRRTRAFSRYSDIPAAI
                     RYAEQFIARMDGGNYPVPSDRLITLENTDEGVVCNVCGGHKANEALARTLSVLLSARY
                     GTTVGIEVGAYRFLLRLPPAVRALEVEETLAALEPAHLPGVLKLALKRTALFKWKLVQ
                     VAKKFGAIDADADYERFSIHRLIDLFDETVIAAEAYRELFSGSMDVDAAREILLAIRD
                     GRTAVTTGRLSPLGSEGLSSSRDMIPPPMIDQAVIGTLMRRLDKEDVVLFCMHCRKWK
                     SRTVVERVPDKPQCPLCNARLIAALKPWDEQFIPAMRKKEKTEEERAIEVRFLRNANI
                     VLSSGKKAVTALAAKGVGPEAASRILATLSEGDAFYREILKAERNYVRTHRFW"
     gene            complement(3795..4550)
                     /locus_tag="PLI76_06600"
     CDS             complement(3795..4550)
                     /locus_tag="PLI76_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOI61736.1"
                     /translation="MHLHFIESGPALLVEQDRRVLVVADLHMGIESGLERHGVHIASR
                     SAVRIARVVACIEEMKPDLLLLLGDVKHNVPVTSRQEYRELPGTLATLRERVPLAVVP
                     GNHDGGIGRFLEPGELLPPGGAVIDGVGYLHGHTRPDPELFGRLIILGHHHPVVSLVD
                     AVGCAARARPAYLYSGLDAGCEVETPPGGNRTRLLFIPAFNEFAGGIDVGRLSESGLG
                     PLSRCIERETAEVFLADGTYVGSPATLCSAGGG"
     gene            complement(4557..5327)
                     /locus_tag="PLI76_06605"
     CDS             complement(4557..5327)
                     /locus_tag="PLI76_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61737.1"
                     /translation="MEPVERVFARDFAAARHTVDSEGGRGGSYVVTPGGAWCRRLFIV
                     GALTERRGSGDVMQARVADPTGTFRVSMDRQTPDAGETLGYIEPPAFVAVTGRATIVG
                     SGPRAYATVDAEALRVVDRTARDLWVQKTAGTTLDRLIALGEALSGDGDERAATTLRL
                     YGTTEEAIRDMAGMVRTALAVVRPGTVPGDIGFVPRRDILLDALEEEGGTRGVPVEDA
                     VAAGVRSGLSAREARAALEELLAAGECYQPASGMVKLI"
     gene            5653..6621
                     /locus_tag="PLI76_06610"
     CDS             5653..6621
                     /locus_tag="PLI76_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication factor C small subunit"
                     /protein_id="HOI61738.1"
                     /translation="MDGNHTIWIEKYRPRILDEMVGQKDIVVRLQSYVKTGNLPHLLF
                     TGSAGIGKTTAAVALAREFFGDSWQTNFREMNASDERGIDVVRNQIKQFARTSPLAGA
                     KFKILFLDEADALTTDAQAALRRTMETYARTCRFILSCNYSSKIIDPIQSRCAIYRFR
                     PLDREAVIEETRRIAAVEGLTITEGALDAIVYVASGDMRKAINALQGAAILRPDIDED
                     LIYEITATARPAEIDELIDLSLAGKFDEAEQALSELTRGRGIAPNELINQCYRALVRR
                     DIDRTLKVRLIDALGETDFRLSEGASSDIQMEALLARFVLAAGEHR"
     gene            6634..9816
                     /locus_tag="PLI76_06615"
     CDS             6634..9816
                     /locus_tag="PLI76_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HOI61739.1"
                     /translation="MPEEEVTVEVTDVVGEWTKILKKQFKRELAELSREYPHKRSLII
                     DFRKILNNRLAFELLRSPGKVLGDIRDAIVQNRLLKLKDGQDPDLINIRFTNLPQKTD
                     VRDIRADQINTFIGIEGILRKTTEVRPRIVSAVFRCRSCGKNTDPVPQGYGRFDEPDV
                     CPNCERKTRLDLVMNRCRFVDAQKLRIQESPEGLRGGEQPQTLDIDVTDDLTGMVSPG
                     DRVVVNGILRSVQRVNYGQKSTLFDIYLECNSIEVAEKEFEEVSITEEDEVKIKALAR
                     DPLVYKKIARSIAPTIYGTDDVKEAIALQLFGGIAKEMPDGSRLRGDVHVLLVGDPGI
                     AKSQILRYVVKLSPRGIYTSGKSSTSAGLTATAVKDEFGDGRWTLEAGALVLADMGIA
                     AVDEMDKMAKEDRSALHEAMEQQCYDDKTEVLTESGWKLFCDVTADDRVATLSSEGRL
                     EYTAPSNFVATEYDGEMYYVKSRQVDLAVTPNHRMYVNLNRRADEWEGFRLVRMDEIP
                     IHKRMRFKKGAIWAGERRDVYEIPPVVKFANQNAEGKLTDPVTVGMNDWLEFLGYFLS
                     EGSVQRHSKTGVPYRTIISQKTSESAETIRECLERLPFRFSYDGRNFSINAKQLAEHL
                     APFGKCHEKYVPGCAKRLPPEQIGILLDALMLGDGYVNKTTGVSIYTTSSKRLADDVT
                     ELLLKKGWSGNVHLMREAGTIIANPRGGTSTTTHDIFQVTFIRDGQNEPNINSNGKRH
                     IEKRPYKGTIYCLEVPNHILYVRRNGIPVWCGNSISVAKAGITATLKSRCALLGAANP
                     KLGRFDQFVPIGEQIDMPPSLLSRFDLIFVMTDQPEAQRDGAIAQHIIKTHAVGELIK
                     QHEFSPLPGVDDAYIERALAPVTPDIDPTLLRKYIAYAKRNCFPILSDGAKEALIAYY
                     MRLRNLAGGNKPVPVTARQLEALVRLAEASARMRLSNTVDTEDTDRILTIVDACLRQV
                     AYDAESGSFDIDKLVTGVTKSQRDIIRSVKEAIRNVSGDSGGQARVDEVIDILLQQGF
                     VRDKIEHTIEQLKRGGEVLEPRHGLVKLIG"
     gene            complement(9840..10913)
                     /locus_tag="PLI76_06620"
     CDS             complement(9840..10913)
                     /locus_tag="PLI76_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOI61740.1"
                     /translation="MAFDRVLNVAKKEFSDHITSRRFIIILALFLVISTVSIHEGIEL
                     YNTRLEMYNEQLQQMTAGFEGLYPGQMPEKPSIMLVFLFMMGYMTALGSILALAVGFD
                     LVSKEKESRSLKSLLSHPVYRDEIINGKMLGGVGALGFAMALALALSLAMLLVFSVVP
                     TLDEFAAILIFGAASLGLLLAYFAIALTMSTVAKESGNALIYTLVIFAIVSTLLPAFG
                     TMAADVFAGDPPEPPETVVPMPTPAQSSGGGYVTADDPRETDPAWKDYEEARKTYIEK
                     RRLISDISSILSPQANYVMVSLAVTDPGSSTMYTAYGAVQAEPGGLADALGRIWMNIA
                     AIIVFPAAFFAATYVKFMRMDIR"
     gene            complement(10917..>11362)
                     /locus_tag="PLI76_06625"
     CDS             complement(10917..>11362)
                     /locus_tag="PLI76_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI61741.1"
                     /translation="LDEPTANLDPEGVREYRDLVERLAGEGKTVFVSSHILSEVRAVC
                     RTVGLLAKGKLVAQGTLDEVARTLAPSDGDAVRIVVETQEHLPDIEDTELLAVERNGN
                     RAVFRAKTDIRDRLAASLLEQGIHVREMRIEEPDIEDIFMAAYRR"
BASE COUNT         2196 a         3595 c         3604 g         1967 t
ORIGIN      
        1 ccttctttgc ccatgcgtcg atcacgtcaa agccgaagtt atagtattca gggacgtcta
       61 tcttgaaatt cgcgcagagg tcttcgtagg atgctacttt ttgctcgtca tctgtcatac
      121 cgcgatcaat agagatttgc gcgcctatgg tttaaagaat tcacgacgat gatgagcgct
      181 ccctattcag aatacccata aagatcatcc attagtatta tggtccgaaa aacgatactc
      241 atatcaatgg atgagaaggg atacgagtca ctgaaaatcg agaacatcgt tgcttccggg
      301 gtgattgccg actcaatcga cctcgaaaaa gtatctaata aaataaaaaa ctgtgaattg
      361 aatacgaaga ggtttcccgg ggcggtctac cgcatcgaga agcccaagat tgcgtccctg
      421 atattctcgt cggggaaggt ggtgcttacc ggtatcagga acaaggagga tctccacgct
      481 ggccttgata tcatcatgca ttcgctccgg gatgccggca tcgatactta cgatgaaccg
      541 caggtcgcgg tcacgaacat cgtctgttcg tacgatatgg gcaggtacat caacctgaac
      601 aaggtggtca tcaccctcaa cctcgagaac atcgagtacg aacccgaaca gttcccgggg
      661 cttgtctacc gtattgagga tccgaagatc gtcgcccttc tcttcagttc cggcaagatc
      721 atcctgaccg gcggaaagaa tatcgaggat atcaagagag ggcttgattt cctggagcag
      781 cggctcgaaa ctatcatgtg agactggttt tcactacact ttttctgagg ggagagagga
      841 gaccgaaagc ccctccgggt tttttgccgg gcgcgatccg gctgtcggga tctcacccgt
      901 atatgccgct ggattctccg ggctaaaagc cggctcttat gcctccggca acccggtgac
      961 gctctcctct cgcatctgcc gccagtctca cgccgggcat cagggcaagc ctgatgcggt
     1021 gaacccgggg tattgctatg ggggatgaag gtcaggcccc gatcttatgc ggaagagcgc
     1081 ggagtatccg ccacaccggc ccggcttctg tctcctctta ccagaacctg tgtgtcctga
     1141 cgtagttacg ctccgccttc aggatctccc ggtagaaggc gtccccctcg gagagggttg
     1201 cgaggatccg ggatgcggcc tccggcccga cgccctttgc ggcgagggcg gtcactgcct
     1261 tcttgccgct cgagaggacg atatttgcgt tccggaggaa ccggacctcg atcgcccgct
     1321 cctcctccgt cttctccttc ttcctcatcg ccgggatgaa ctgctcgtcc cagggtttca
     1381 gcgcggcgat gagacgtgcg ttgcagagcg ggcactgcgg cttatcaggg acccgttcga
     1441 cgacggtccg gctcttccac ttccggcagt gcatgcagaa gaggacgacg tcctccttat
     1501 cgaggcgccg catcagcgtc ccgatcaccg cctgatcgat catcggcggc gggatcatgt
     1561 cccgcgacga ggagagccct tcgctcccga gcgggctcaa cctccccgtg gtgacggcag
     1621 tccggccgtc gcggatcgca aggaggatct cccgggccgc gtccacgtcc atcgagccgc
     1681 tgaagagctc ccggtaggcc tcggccgcga tgacggtctc gtcgaagagg tcgatgagcc
     1741 ggtggatgct gaaccgctcg tagtcggcat ccgcgtcgat ggcgccgaac ttctttgcca
     1801 cctgcacgag cttccacttg aagagcgccg tccgcttcag ggcgagtttc aggaccccgg
     1861 ggagatgtgc gggctcgagc gccgcgaggg tctcctccac ctcaagcgcg cggacggccg
     1921 gcggcaggcg gaggaggaac cggtaggcgc cgacctcgat cccgacggtc gtcccgtacc
     1981 gggccgagag gaggaccgag agcgtccggg caagcgcttc gttcgccttg tgccccccgc
     2041 agacgttgca gacgacgccc tcatccgtat tctcaagggt gatgaggcga tcggaaggga
     2101 ccgggtagtt gcccccgtcc atccgggcga tgaactgctc cgcgtaccgg atagccgccg
     2161 ggatatcgga gtagcgggag aacgcccggg tcctccggag cgccccgacc tcccgggcaa
     2221 cggcgaacgg caccgggatc tgctcgccct cccacgtcgg tacctcgccc cgcgcccggg
     2281 cggccggctc gaccgttatc cgccctccct cgatatcaag gacctgccag agctgccctt
     2341 tcgtgatgaa gatagcgccg gtgtgcatcc agccgaggac gaacgactcg tcgagcgtcc
     2401 cgaccgtgcg gcgcgagacc atatcgaaga tctccacctt ccgctcgtcg gggatcatcg
     2461 agaggttatc gatgaggtaa cgccgggcac gcccggtccg ggtaacccgt gtcccgtcga
     2521 gccggatcag ccggtgctcc gccatctggc tgcagacggt atcaaggagg gggccgcacc
     2581 cggcaaagac tcctgagcgc tcgatcatat cccggatgcg ggagacctcg atctcgccgt
     2641 actcgaccgc gattgccgct acctggtttg caaggacgtc ggcggcgccg gcgggcggga
     2701 cgatccgctc gcactcgttc gcttttgccc ggcgcgcgat cacgagcgac tctaagaggt
     2761 cgtcaaaccc cgtggcgagg acggtcccgc gcgatattgc atcgagacga tggccggccc
     2821 ggccgacccg ctgcaccagg cgcgagacct gccggggaga accgaactgg acgacgtgct
     2881 cgatgtgccc gatgtcgata ccgagttcca tcgacgatgt ggcaatcaac gtccgaaccg
     2941 cgccctccct gaaccgttct tcggcctcga ccctgacgtc ccgcgagagc gacccgtggt
     3001 ggacctcgac gtcgccgcgg ggatagagct cgtgcccgag agcctcggcg gtcacccggg
     3061 tgttgacgaa gacgagggtg gaccccgggg cgtcgaggca atctccgacg gcccgcgtct
     3121 gggcggcaaa atcctccccg gcaaaccgga cgccgatctc gaggtggctc gccaccggca
     3181 cctcgacgag cgagaacgcc cgggccccgc agaggaaccg cccgatatca tccgggttcc
     3241 cgacggttgc cgagaggccg atccgctgga actctcccgc gtaggcggcc aggcgctcga
     3301 gcgcgaccgc gagctgggcc ccccgtttgc tgtcggcgag ctcgtggatc tcgtcgatga
     3361 tgacgtacct gacgttcttt aagtgttcac ggaggcgttt gcccatgaag agcgcctgca
     3421 gggactcggg cgtcgtgatc aggaggtcgg gaggattgag cgcctgtttc cgccgttcgt
     3481 tctgcggcgt atccccgtgc cggacaccgg ccgagagcga gagttcccgg caccaccact
     3541 ccatccgcgc aagtatatcg cggttcaacg accggagagg ggtgacgtag accgccttga
     3601 accccgggcc ggcggtcgca aggagccggt cgaagaccgg aagcatcgcg ctctcggtct
     3661 tccccgtgcc ggtcggggcg accaggacca ggtttttgcc ctcgagcagg agcgggatgg
     3721 cctgttcctg tgcatcggaa agagcggtaa acccgcgctt ccggacgacc tcccggaccg
     3781 cggggctgag gagttcaccc gccgccggcg gagcagagcg tggccgggga gccgacgtac
     3841 gtaccatctg ctaagaacac ctccgcggtt tctctctcta tacaccggga gagggggcca
     3901 agcccgctct ccgaaagccg cccgacgtcg ataccccccg caaactcgtt gaaggccggt
     3961 ataaagagga gccgggtgcg gttgccgccg ggcggcgtct ccacttcaca ccccgcatca
     4021 agccccgagt agaggtaggc cggccgggcg cgtgcagcgc acccgacggc atccacgaga
     4081 gagacgaccg ggtggtggtg gccgaggatg atgaggcggc cgaagagttc ggggtcgggg
     4141 cgggtatggc cgtggaggta gccgacgccg tcgatgaccg cgccgccggg gggcaggagt
     4201 tcgccgggtt cgaggaaccg cccgataccc ccgtcgtggt ttccggggac gacggcgagc
     4261 ggcaccctct cccggagagt cgcaagggtg ccggggagtt cacggtattc ttgccggctc
     4321 gtcaccggga cgttgtgctt gacgtcgccg aggaggagga ggagatcggg cttcatctcc
     4381 tcgatacagg caacgacccg ggcgatccgg acggcgctcc ggcttgctat gtggacgccg
     4441 tggcgttcaa ggcccgactc gatccccatg tgcagatcgg cgacgacgag cacgcgccgg
     4501 tcctgctcga cgaggagggc aggaccgctc tcgataaagt gcaggtgcat aacgtgttat
     4561 atcagtttta ccatgcccga tgcaggctgg tagcactcgc ctgcagcaag gagttcctca
     4621 agcgccgcac gtgcctcccg cgcggagagg ccggaacgca ccccggcggc gacggcatcc
     4681 tcgacgggga caccccgggt gccgccctcc tcctcgagcg catcaaggag gatgtcgcgc
     4741 cgggggacga agccgatatc gccgggaacg gtacccgggc ggacgaccgc gagcgccgtg
     4801 cgcaccatcc cggccatatc ccggatcgct tcctccgtcg tcccgtagag ccggagggtg
     4861 gttgcagccc gctcgtcacc gtcgcccgat agagcttctc cgagcgctat gaggcggtcg
     4921 agcgttgtgc cggcggtctt ttgcacccag agatcgcggg cggtgcggtc gaccacgcgg
     4981 agcgcctcgg catccaccgt tgcgtacgcc ctcggcccgg aaccgacgat cgtcgcccgg
     5041 ccggtgacgg cgacgaacgc cggcggttcg atgtacccga gcgtctcgcc cgcgtccggg
     5101 gtctgccggt ccatcgagac cctgaacgtg ccggtcgggt cggcgacccg ggcctgcatg
     5161 acatcaccgc tccccctgcg ctcggtgagg gcgccgacga tgaagaggcg gcggcaccag
     5221 gcaccgccgg gcgtcacgac gtacgagccg ccacggcccc cctcggagtc taccgtatgc
     5281 cgcgcggcgg caaagtctcg cgcaaatacc cgttctacag gttccatatc agccgtctat
     5341 tccctgagtg cgtcgagtcg ccctgatgcg agggcatcga tcggtgagga gggtcgtcat
     5401 gaccctgcag tctttgaggc agcaggcgaa gcgattgggc ggggatgccg gcgcggccgc
     5461 tcgcgctgcc gtgaaggggg aaaccaggga tgcgcgggtt tatcgggcct tatcgaagac
     5521 cggatctcca cgcgaactcc cggtatctcc acagtaatct gtacctatcc tttctctctc
     5581 aaccctctaa aaaggatcac cggaggcgat gtcgatgctt ttacaatgcg cgagcgccaa
     5641 cacttcatgc aaatggacgg taaccacacc atctggatag agaagtatcg gccccggata
     5701 cttgacgaga tggtcgggca gaaggatatc gtggtgcgcc tccaatccta cgtgaagacc
     5761 ggcaacctgc cgcacctcct cttcaccggg agcgccggga tagggaagac gaccgctgcc
     5821 gtggcgctcg cgcgggagtt cttcggcgac tcctggcaga cgaacttccg ggagatgaac
     5881 gcatccgacg agcgcggcat cgacgtcgtc aggaaccaga tcaaacagtt cgcccggaca
     5941 tcgccgcttg ccggcgcgaa gttcaagatc ctcttcctgg atgaggcgga tgcgctcacg
     6001 acggatgcgc aggccgccct ccggaggacg atggagacct acgcccgcac ctgccggttc
     6061 atcctctcgt gcaactactc ttcaaagata atcgatccca tccagagccg gtgcgccata
     6121 taccggttca ggccgctcga ccgcgaggcg gtcatcgagg agacccggag gatcgccgca
     6181 gtcgaaggcc tcaccatcac ggaaggggcg ctcgacgcca tcgtctatgt cgcttccggg
     6241 gatatgcgaa aggcgatcaa cgccctgcag ggtgccgcca tcctccggcc cgatatcgac
     6301 gaggatctga tctacgagat caccgcgacc gcccgcccgg cggagatcga cgagctcatc
     6361 gacctctcgc ttgcagggaa gttcgatgag gccgagcagg cgctctccga actgacccgc
     6421 ggccggggca tcgccccgaa cgagctgatc aaccagtgct accgggcgct tgtccggcgc
     6481 gatatcgacc ggacgctcaa ggtgagactg atcgatgccc tgggcgagac ggacttccgt
     6541 ctctcggaag gggcaagcag cgatatccag atggaggcgc tgctcgcccg gttcgtcctc
     6601 gccgcaggag agcaccggtg aggggctccg gccgtgcctg aagaagaggt aaccgtcgag
     6661 gtcaccgatg tcgtcggtga gtggacgaag atcctaaaaa aacagttcaa gcgggagctc
     6721 gccgagctct cccgcgagta cccgcacaag cggtcgctca tcatcgattt tcgaaagatc
     6781 ctgaataaca ggctcgcgtt tgagctcctg cggagcccgg ggaaggtcct cggcgacatc
     6841 cgggatgcga tcgtccagaa caggctcctc aaactcaaag atggccagga tccggacctg
     6901 atcaacatcc ggttcacgaa cctgccgcag aagaccgacg tccgggatat cagggccgac
     6961 cagatcaaca cgttcatcgg catcgagggg atcctcagga agacgaccga ggtccgcccc
     7021 cggatcgtga gcgcggtctt ccggtgccgc tcctgcggga agaacaccga cccggtcccc
     7081 cagggctacg ggcggttcga cgagcccgac gtctgcccga actgcgagag gaagacccga
     7141 ctcgatctcg tcatgaaccg ctgccggttc gtcgatgccc agaaactccg gatccaggag
     7201 tctcctgaag gcctccgcgg cggcgagcag ccccagacgc tcgatatcga cgtcaccgac
     7261 gacctcaccg ggatggtctc gccgggcgac cgggtggtgg tgaacggcat cctgcggtcg
     7321 gtgcagaggg tcaattacgg ccagaagagc acgctctttg atatctacct ggagtgcaac
     7381 tcgatcgagg ttgccgagaa ggagtttgaa gaggtctcga tcaccgagga ggatgaggtg
     7441 aagatcaagg cgctcgcccg cgatcccctg gtctacaaga agatcgcccg gtcgatcgcg
     7501 ccgacgatct acgggaccga cgacgtgaag gaggcgatcg ctctccagct cttcggtggt
     7561 atcgcgaagg agatgccgga cggctcccgc ctccgcggcg acgtccacgt cctcctggtc
     7621 ggcgacccgg gcatcgccaa gagccagata ttgcgctacg tcgtgaagct ctcgccccgc
     7681 gggatctaca cgagcggcaa gtcgtcgaca tccgccggcc tgacggcgac ggccgtgaaa
     7741 gacgagttcg gcgacgggcg ctggacgctc gaggccggcg cgctggttct tgcggatatg
     7801 gggatcgccg ccgtcgacga gatggacaag atggcgaagg aggaccggag cgcgctgcac
     7861 gaggctatgg aacagcagtg ctacgacgac aagactgagg ttctcacgga gagcgggtgg
     7921 aagctcttct gcgatgtcac cgctgacgac cgcgtcgcga ccctctcctc tgagggcaga
     7981 ctcgagtaca cagccccgtc caacttcgtg gcaacggagt acgacggaga gatgtactac
     8041 gtcaaatccc ggcaggtcga cctcgcggtc accccaaacc accggatgta cgtcaacctg
     8101 aaccggcgtg ccgacgagtg ggaggggttc cggctcgtcc gcatggacga gatcccaatc
     8161 cacaagagga tgcggttcaa gaaaggcgcc atatgggcgg gagagcggcg agatgtctac
     8221 gagatcccgc ccgtcgtcaa gttcgcgaac cagaacgccg aaggaaaact caccgatccc
     8281 gtcaccgtcg ggatgaacga ctggcttgag ttcctgggtt acttcctctc cgaggggtcg
     8341 gttcagcggc actctaagac cggggtgccc taccgcacaa tcatatcgca gaagacctct
     8401 gagtcggccg agacgatccg ggagtgcctc gaacggctgc cgttccggtt ctcctacgac
     8461 ggcagaaact tttcgatcaa tgcaaaacag ctcgccgaac atctcgcgcc gttcgggaaa
     8521 tgccacgaga agtacgtccc cggctgcgcg aaacgtctac ccccggagca gatcgggata
     8581 ctcctcgacg cgctcatgct cggggatggc tacgtcaaca aaacgaccgg ggtctcgata
     8641 tacacgacct cttcgaagag actggccgac gacgtcaccg aactcctgct gaagaaagga
     8701 tggtccggga acgttcatct catgcgggag gccgggacga tcatcgcaaa cccccggggc
     8761 ggaacctcga cgacgaccca cgacatcttc caggtgacgt tcatccgcga cggtcaaaac
     8821 gagccgaaca tcaattcaaa cgggaagcgg catatcgaga aaaggccgta taagggcacg
     8881 atctactgcc ttgaagtccc gaaccacatc ctctatgtcc ggcgtaacgg catcccggtc
     8941 tggtgcggca actcgatctc ggtcgccaaa gccggcatca ccgcgaccct gaagtcccgg
     9001 tgcgcgcttc tcggtgcggc gaacccgaaa ctcgggcggt tcgaccagtt cgtccccata
     9061 ggcgagcaga tcgatatgcc gccctcgctc ctctcgcggt tcgacctcat attcgttatg
     9121 accgaccagc cggaggccca gcgcgacggg gcgatcgcgc agcacatcat caagacccat
     9181 gctgtcggcg aactgatcaa gcagcacgag ttctcgcccc tgccgggcgt cgacgatgcg
     9241 tatatagagc gtgcccttgc gccggtcacc cccgacatcg atcccacgct tctccggaag
     9301 tacatcgcct acgcgaagcg gaactgcttc cccatcctct ccgacggggc gaaggaggcg
     9361 ctgatagcct actacatgcg cctgcgaaac ctcgccggcg ggaacaagcc cgttcccgtc
     9421 actgcccggc agctcgaggc gctggtccgg cttgcggagg cgagcgcacg gatgcgcctc
     9481 tcgaacaccg tcgacacgga ggacaccgac cgaattctca cgatcgtgga tgcctgtctc
     9541 cggcaggtcg cctacgacgc cgagagcggg agcttcgata tcgacaaact cgtgaccggt
     9601 gtcacgaagt cgcagcgcga catcatccgg tcggtcaagg aggctatccg gaacgtctcc
     9661 ggcgactccg gcggccaggc aagggtcgat gaggtgatcg atatactgct gcagcagggc
     9721 tttgtccgcg acaagatcga gcacaccatc gagcagctga aacggggcgg cgaggtgctc
     9781 gaaccccggc acgggctggt gaagctgatc gggtaggctg caatgcaggc acccgatctt
     9841 catctgatat ccatccgcat gaacttcacg taggtcgcgg cgaagaaggc cgcagggaag
     9901 acgatgatcg cggcaatgtt catccagatc cggccgaggg catcggcaag cccgccgggc
     9961 tccgcctgca ccgcgccata ggccgtgtac atcgtcgagg atccgggatc cgtcaccgcc
    10021 aatgagacca tcacgtagtt cgcctgcggc gataggatgc tggagatgtc gctgatgagc
    10081 cgtcgcttct caatataagt ctttctcgcc tcctcgtaat ctttccacgc cggatcggtc
    10141 tcacgggggt cgtcggcggt gacgtatccc ccaccggaac tctgtgccgg ggtcggcatc
    10201 gggaccaccg tctccggtgg ttcggggggg tcgccggcga aaacatccgc ggccatcgtc
    10261 ccgaaggcag ggaggagcgt ggagacgatg gcgaagatga cgagggtgta gatcaacgcg
    10321 ttcccgctct cttttgctac cgtcgacatc gtcagtgcga tcgcgaagta ggcgagcagg
    10381 agcccgagcg aggccgcccc gaagatcagg atggcggcga actcatccag cgtcgggacg
    10441 accgagaaga cgaggagcat cgcaagtgag agggcaagcg cgagcgccat ggcgaacccg
    10501 agcgcgccga cgccaccgag catcttgccg ttgatgatct cgtcgcggta gaccgggtgg
    10561 gagaggagcg acttgagcga ccgggactcc ttctcctttg agaccaggtc gaacccgacg
    10621 gcaagagcaa ggatactacc gagggccgtc atgtaaccca tcatgaacag gaagacgagc
    10681 atgatcgagg gtttttcggg catctggccc gggtaaaggc cctcgaaacc cgctgtcatc
    10741 tgctggagct gctcgttata catctccagg cgggtgttat acagttcgat gccttcgtgg
    10801 atgctgaccg tggatattac caggaacagg gccaggatga tgatgaaccg cctgctggtg
    10861 atgtggtcgg agaactcttt ctttgctacg ttgagcactc gatcgaacgc catgctttac
    10921 ctccgatagg ccgccatgaa gatgtcctcg atatccggct cctcgatccg catctcccgg
    10981 acgtgaatcc cctgttcaag gagggaggct gcaagccggt cccggatgtc ggtcttcgcc
    11041 cggaagaccg cccggttccc gtttcgctcg acggcgagga gctcggtatc ttcgatgtcc
    11101 ggcaggtgct cttgggtctc gacgacgatc ctgacggcgt cgccgtccga aggtgcgagc
    11161 gtccgggcga cctcgtcaag cgtcccctgc gcgacgagtt ttcctttcgc gaggagcccg
    11221 acggttcggc agaccgcacg gacctcggag aggatgtggg aggagacgaa gaccgtcttt
    11281 ccctcgccgg cgaggcgttc gacgagatcc cggtactccc ggacgccttc ggggtcgagg
    11341 ttcgccgtcg gttcgtcgag ga
//
LOCUS       DAOSEE010000070        11209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_2223_length_11209_cov_229.760086, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000070 DAOSEE010000000
VERSION     DAOSEE010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 11209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11209
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2223_length_11209_cov_229.760086"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PLI76_06630"
     CDS             complement(<1..285)
                     /locus_tag="PLI76_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61742.1"
                     /translation="MRCAKAFLGVCFLLAVLPAGALAGTPLWTHSISSDIADLSLTPD
                     GSFILVGGDRLCLLTGNGTLLWREWAAGLIACSADGRLIAGAAGPSLILFN"
     gene            469..735
                     /locus_tag="PLI76_06635"
     CDS             469..735
                     /locus_tag="PLI76_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61743.1"
                     /translation="MADAKKYYAAAVLAVWLIIIAFFMVLSRTLDLEVFFVLALIGLL
                     VVAVLIDGAFSRPRYMRYIGYIIAAGVALFAYIVAQKILEILAA"
     gene            732..2360
                     /locus_tag="PLI76_06640"
     CDS             732..2360
                     /locus_tag="PLI76_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOI61744.1"
                     /translation="MNRQVSHAVLAAVAILVLVLIGVQAVDPVLYAAKNISTAAHADP
                     ATIQKRSLGDAASLVPLMDDLLGQTGTLALTIKLKDYESAERDLARYNELSRRFDRLV
                     VTLDVSGTDIGEFQQNNRQNQDALAALLNDTRRFDALQRLEIEVRGDDEQHMTVIYEG
                     EALRQKMRETFSAYARREAATVRIAEHYEINATPYQESVDHFAEVADAANGWQEEING
                     GGTGTGGTGSGGTVQSPLVIAVTPVEGSYGDVLLIGGIHAAGKSGAPVEIYVDSRLAA
                     TVALDDDRTYVHSYRVGRILAGPHLAYATAGTIYSNVAAFEVLPKNTSITLSLAEVNG
                     TAVACTGNLTADDGRPVTRAPVLIRVDGETLVGAETDGNGTYRADILLPAGEHALRAE
                     FHAAGYPLNASESPTETVILRGEGLSPFPFAAAIAAAAGTGWYLRRRRSEESTPAAEP
                     EAAPSPEVARPTPQVSIAGLPPREAATVLFCTLRARLGLPETKTPRDCARIAPAYAGF
                     FERYERIRYAGETPSEEELTDMERIALGGDADRA"
     gene            2341..3162
                     /locus_tag="PLI76_06645"
     CDS             2341..3162
                     /locus_tag="PLI76_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HOI61745.1"
                     /translation="MTQIEREAWAILLILLLAAAVAYAHVTTTTEEYSRYNTDWNGTS
                     ALPAEEIRDLRNLDTAATLLILAPDRPFTAEEVGYLQTFLDAGGRVIVADEEGTANSL
                     LADLGSTMRVRPQNLASLERDRADPGLFTVSVVGNTSLFAGVGTVRVNRPAAVTGGEP
                     LLETAILTWEDGDGDGRISGDEVFGTAVICAAEGNLTVLGDESLFINAMLAENPQFIE
                     NIQPIRVDSIHSRTGTANPIINTRLWIQETPPAAAAAAALAILPVAWHFGRKRDD"
     gene            3155..4126
                     /locus_tag="PLI76_06650"
     CDS             3155..4126
                     /locus_tag="PLI76_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOI61746.1"
                     /translation="MIDDLTTRINAIADAYEEIRATTQQVIVGNQPLIEEIFICMISG
                     GHLLIEGVPGTAKTTVCKIVAHLIDYDFRRVQGAVDLQPADIIGIRIYDREKSTFALQ
                     RGPIFTNFLMVDEMNRLTPKTQSALLEAMSERQATIDGITYPLPDPYFAIATQNPHEI
                     EGTFPLIEAQRDRFMFSSVLDHLNSENELEILRREHAGELDWKVYRDRVDPILDPTGV
                     EAMAAAIREVRVDETVLGYMRDLVLATRNHGDVRLGASSRASIALLRGSAARAALNRR
                     TYVIPDDVRALAPSTLRHRLLLTREAAITGVTAEAIIADIVESVGVS"
     gene            4129..5373
                     /locus_tag="PLI76_06655"
     CDS             4129..5373
                     /locus_tag="PLI76_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOI61747.1"
                     /translation="MIRPTRTSGGIAALALALAVTALLLENPAAAFAAGSLALFLLWR
                     GWRFESDLAAAATSLEVIRTVDRTILRQGTAATVRVQINLTVPPGMDIRVRDVPPAVA
                     VGDAPPSPPGRAVTYTIRLMAPGETSFGGVAIEAADTFFTTNLVCRRSGAPQIRVFPA
                     GTVGDGRGRGAGSGDTEVDRRMAISGQSILGFRPYQTGDDPNLIDWKITARRDTLYVR
                     QMTGLEGGAPLIAVDLPGRAGDPAAFARFSMAVSGAVEGAISARDGCSLLIVAGPEIV
                     RFLPRTTEIREALAALGGLAPLEPRAPLYRAPGPAVLAARARVPRQGRGEEETAYLAR
                     LCGALSAFAGENPALFAAAVRTAFSVAEAREVRVYSLLPPGDRSHLIQLAGEAKIRGM
                     RVVLKAPAGTGTLPGVDTVEVI"
     gene            5373..5660
                     /locus_tag="PLI76_06660"
     CDS             5373..5660
                     /locus_tag="PLI76_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61748.1"
                     /translation="MEIDRKFLLLCAAEVLVVAVDNLILALILQVIALGAFVGDRRGY
                     PVFVAGAILFAAVVASAGNVALIGLAAALGCGYLILTLYDYRLTRWAGGEA"
     gene            5657..6448
                     /locus_tag="PLI76_06665"
     CDS             5657..6448
                     /locus_tag="PLI76_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61749.1"
                     /translation="MTTKSPYRGAFILIIAAAALLALAALTGRGDITTATLVLAGTGC
                     FIAGVFLLALHKGEPLDPDIAALLPAQGTIGIATLCADLGVQGNAWFVPEEETVIEVI
                     PVADTLPEKTGDDYSFITREGECAVRLAPACTPLLARLREKHALEVPEENEALLACIT
                     EVCDDLLEIAEKTEAKRDGENIVVTLSGYRLIPGCRTIRAASPKCCTMVACPVCSLIA
                     AIAALGTKMPVTIEHIDVDEKRKSLRMILTPAGPAPRGADPGNSR"
     gene            complement(6396..7370)
                     /locus_tag="PLI76_06670"
     CDS             complement(6396..7370)
                     /locus_tag="PLI76_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1616 domain-containing protein"
                     /protein_id="HOI61750.1"
                     /translation="MDTEDRQPLILEAAGDVPGDLVAIAAVTAITLACVYVPVLNESF
                     LRILFGVAMVLFIPGYALIAALFPARGDLDGIERVALSFGLSIAVVPLIGLALNYTPW
                     GIRLDPILASLTLFTLAMTAAAWYRRLLLPAGDRFVVPARAMLAAARLEFFDPGASRL
                     DRGLSALLLVSIVAALATTAYVIAVPKEGEHFTEFYILGPGGKAADYPTDFPAGATRS
                     LIIGVGNHEYREIPYTVEAFALNQTFDTTTNTSTIHAVERLDRFVVTVPHNETEEIPW
                     EFAVPSTAYNRIEFLLFNETVPGEDIMGQDRINASYRDLHLWVRVRPA"
     gene            complement(7382..8566)
                     /locus_tag="PLI76_06675"
     CDS             complement(7382..8566)
                     /locus_tag="PLI76_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61751.1"
                     /translation="MKRLGGFLLLLVLLIPAATAATITISPDTIDSGDTVTVTVNDLP
                     DGASFSLGIRGEFAATPGEEFAFSARGITLPFALNGGEVNAYTKGTNWTELAANLPDG
                     GSVILLKYADPNNEVRISQSQNIGSGTISLVTLRGEAAADTIIADFTVMGTKKGPIDG
                     TISFALEGIEQGTATVSVYINGSEALARTITIGGGVPTTPTTTTTSTPAPTTTESGNG
                     DSSGTPGGSSGGSTGPVSTTAPTTATVTSTDGKASLAGTDTAGAGLLAVAAQGTTPAG
                     WTFAGNAYAVTPANRTFDPAVVLSFTLPSAGATATIARLDNGAWSPVPSKVEGDRITA
                     EISRAGTYALLVPAEAPTATTIPATTTAVPVTTTPTAAPLTALLPVIAFATLMLERKR
                     RG"
     gene            complement(8652..9587)
                     /locus_tag="PLI76_06680"
     CDS             complement(8652..9587)
                     /locus_tag="PLI76_06680"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HOI61752.1"
                     /translation="MDTRALRAFIRLGRFPFLLSGFIPFTAGALFGFLLGARFTPGEF
                     LFGYAAMAAAHLSVHYSNDYFDADADRFVETTAISGGSGVLAAHPGLKPAALAAAVAL
                     MAASILIGYLFVAVYDYPMIFLAFGIAGNLLGWFYTAPPFALAYNNLGEVANVITFGL
                     LMPGAGYFVATGRLDPAFFAFAIPLALYGLVFIVSVEIPDREGDIAGGKRTLVVRRGR
                     IFGFCLIAAAAALATAAFALFALTGILSPVDFRPVAVFSLVPLAIALRGLSNRSAGRA
                     GALPHAVANIRGYFIFQTLLALYLLITAAGSLLSP"
     gene            complement(9632..10264)
                     /locus_tag="PLI76_06685"
     CDS             complement(9632..10264)
                     /locus_tag="PLI76_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61753.1"
                     /translation="MAYDCGPLDRSIEETLAALRDGLAREYRLYRRPAHRRSPRRTRR
                     LRRIGGWRRAADRLIFEAGRVARETLPRIERDTAHTFPGPDGLLRVLMDPSTKRLFAG
                     VLAGFPEEALPVPARDLACLAAFSDDARALALIGDVTLRLRGFSGPEILVALSDRWEL
                     HESPVGRPAGKTLSSEKEALARAVLGLIYVQGGAGALERAVRDPGCDPAG"
     gene            complement(10319..10582)
                     /locus_tag="PLI76_06690"
     CDS             complement(10319..10582)
                     /locus_tag="PLI76_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HOI61754.1"
                     /translation="MPLLPAAIAAYILVNAVSFAAFRRDKKLAGRAAWRTPERRLLLL
                     AFFGPLGALAAMRVFRHKTQKAKFRLVPLFLCLHVGIVVLLLL"
     gene            complement(10586..11014)
                     /locus_tag="PLI76_06695"
     CDS             complement(10586..11014)
                     /locus_tag="PLI76_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron dependent repressor, metal binding and
                     dimerization domain protein"
                     /protein_id="HOI61755.1"
                     /translation="MKHKGCAKNLSRRVEDYLEAILNVTLEKGYARTKDVACELDLSP
                     SSVVEMFRKLDEMGFVEYRRYEGVVLNPAGREVAEVIKARHDTLKEFLRIIHVPEKIA
                     SADACYMEHELHPETIRQIRLLVEVLRADSGVSERIRRGG"
BASE COUNT         2031 a         3775 c         3652 g         1751 t
ORIGIN      
        1 gttaaagagg atgagggacg ggccggccgc ccccgctatc aggcgcccgt cggcggagca
       61 ggcgatcagc cccgccgccc actcccgcca gagaagagtg ccgttgccgg taaggaggca
      121 gagccggtcc cccccgacca ggatgaagga gccgtccggt gtcagggaaa gatcggcgat
      181 atcggatgat atggagtggg tccagagagg ggttcctgcc agggcaccgg cgggcagaac
      241 cgcaaggagg aaacagacac cgagaaaggc cttcgcacac cgcatacgcc agaaggtttc
      301 tggagcgaca ctataatgat tccgttttac ataaagaggt aaaacgggat ttcatcgaga
      361 tccgcccccc gggcaccggg aacattcgcg attccccgcc atccaccccg ggacgggaaa
      421 acatcatgtc aataagatta tctcttctag agccgaaatt tagtcaggat ggcggatgcg
      481 aagaaatact atgctgcggc cgtactcgca gtatggctca tcatcatcgc cttcttcatg
      541 gtcctatccc gtacgctcga ccttgaggta ttcttcgtcc tcgccctgat cggcctcctg
      601 gtcgtcgcgg tcctgatcga cggggccttc tcgcggccgc gctacatgcg atacatcggc
      661 tacatcatcg ccgcgggggt cgccctcttc gcctacatcg tcgcccaaaa gatactggag
      721 atccttgctg catgaaccgt caggtctcgc acgccgtcct tgccgcggta gccatcctcg
      781 tcctcgtcct catcggagtc caggcggtcg accccgtcct ctacgccgca aagaacatat
      841 cgaccgccgc ccacgccgac ccggcgacca tccagaagcg ctctctaggc gacgccgcat
      901 ccctcgtccc ccttatggat gacctgctgg gccagaccgg gaccctcgcc ctcacgatca
      961 agctcaagga ctacgagagc gccgagcgcg accttgcccg ctacaacgaa ctctcccgcc
     1021 ggttcgaccg gctcgtcgtc accctcgacg tctccgggac cgacataggc gagttccagc
     1081 agaacaaccg gcagaaccag gatgccctcg cggccctctt aaacgatacc cggcggttcg
     1141 atgccctcca gcgcctcgag atcgaggtca ggggcgacga cgagcagcac atgaccgtca
     1201 tctacgaggg cgaggccctc cggcagaaga tgcgggagac tttctccgcc tacgcaagga
     1261 gagaggccgc aacggtcagg atcgccgagc attacgagat caatgccacc ccctaccagg
     1321 agagcgtcga ccacttcgca gaagtcgccg atgccgcgaa cggctggcag gaggagatca
     1381 acggtggtgg aaccggaaca gggggaaccg gcagtggtgg aaccgtacaa tcccccctcg
     1441 tcatagcggt caccccggtc gaggggagct acggtgacgt cctcctcatc ggcgggatcc
     1501 acgccgccgg aaaatccggc gctcccgtcg agatctacgt cgacagtcgg ttggcggcga
     1561 ccgtcgccct cgacgacgac cgcacctatg ttcactccta ccgggtcggc cgcatcctcg
     1621 ccggcccgca cctcgcctac gccaccgcgg gaacaatcta ctcaaacgtc gcagcgttcg
     1681 aggttctgcc caaaaacacc agcatcaccc tttccctcgc ggaagtgaac ggaacggccg
     1741 tcgcctgcac ggggaacctc acggccgacg acggtcgccc ggttacgcga gccccggtcc
     1801 tcataagggt cgacggcgaa accctcgtcg gggcagagac cgatggaaac ggcacctaca
     1861 gggcggatat tctcctcccc gccggggagc acgccctccg tgccgagttc cacgccgcgg
     1921 gctaccctct gaacgcttcg gagagcccga cagagacggt catcctccgg ggcgaagggc
     1981 tctcgccctt ccccttcgcg gcggcgatcg cggcggcggc cggcaccggg tggtacctcc
     2041 gccggcgccg atcggaggag agcaccccgg cggcagaacc ggaagcggca ccgtcgccgg
     2101 aggttgcgag acccacgccg caggtgagca tcgcaggcct cccgccgcgc gaggccgcga
     2161 ccgtcctctt ttgtaccctc cgtgcccggc tcggtctccc cgagacaaag acgccgcgcg
     2221 actgcgcccg catcgccccg gcatacgccg ggttcttcga gcgctacgaa cgaatccggt
     2281 atgcaggaga gaccccttca gaggaggagc tcacggatat ggaacgcatt gccctcgggg
     2341 gtgacgcaga tagagcgtga ggcctgggcg atcctcctca tcctcctcct cgccgccgcc
     2401 gtagcttacg cccatgtcac caccaccacc gaagagtaca gccgctacaa cacggactgg
     2461 aacggcacat cggcgctccc tgcagaggaa atccgcgacc tccggaacct cgacaccgcg
     2521 gcaacccttc tcatcctcgc gcctgacaga ccttttacgg ccgaggaggt cggttacctg
     2581 cagacgttcc tcgatgccgg cggccgcgtc atcgtcgccg acgaggaagg aaccgcaaac
     2641 agcctccttg cagacctcgg gagcaccatg agggtccggc cgcagaacct cgcaagcctc
     2701 gagcgggacc gtgccgatcc cggcctcttc accgtcagcg tcgtcgggaa cacgagcctc
     2761 tttgccgggg tagggaccgt ccgggtcaac cgccccgcgg cggtcacggg gggtgaaccc
     2821 ctcctcgaga cggcgatact cacctgggag gacggcgacg gcgacggccg gataagcggc
     2881 gacgaggtct tcgggaccgc ggtcatctgc gcggccgagg gaaacctcac cgtactcggc
     2941 gatgaaagcc tcttcatcaa cgccatgctc gccgagaacc cacagttcat cgagaatatc
     3001 cagccgatcc gggtagacag catccacagc agaaccggca ccgctaaccc aatcatcaac
     3061 acacgattat ggatacagga gacgccgccg gctgcggctg ccgcggcggc cctggccatc
     3121 ctgccggtgg cctggcactt tgggaggaag agagatgatt gacgacctta ccacacgcat
     3181 caatgcgata gcagacgctt acgaagagat cagggcgacg acccagcagg tgatcgtcgg
     3241 gaaccagccc ctcatcgagg agatcttcat ctgtatgatc agcggcggcc accttctcat
     3301 cgagggggtt cccgggaccg caaagactac tgtctgtaag atcgtggcac acctcatcga
     3361 ttacgacttc aggagggtcc agggggccgt ggatctccag ccggccgaca tcatcggcat
     3421 ccggatatac gaccgggaaa aaagcacgtt cgccctccag agggggccca tcttcacgaa
     3481 cttccttatg gtcgacgaga tgaaccgcct gacgccgaag acccagagtg ccctcctcga
     3541 ggccatgagc gagcgccagg cgacgatcga cgggatcacc tatccgctcc ccgaccccta
     3601 ctttgccatc gccacccaga acccccacga gatcgagggg accttccccc tcatcgaggc
     3661 gcagcgcgac cggttcatgt tcagctccgt cctcgaccac ctcaactccg agaacgaact
     3721 ggagatcctc cggcgggagc acgccggaga actggactgg aaggtctacc gcgaccgggt
     3781 cgaccccatc ctcgacccca ccggggtgga ggctatggcc gcggccatcc gggaggtccg
     3841 ggtcgacgag acggtccttg gctacatgcg cgatctcgtc ctcgccacgc ggaaccacgg
     3901 cgacgtccgg ctcggggcga gctcccgtgc ctcgattgcc ctcctacgag gctcggccgc
     3961 ccgggcggca ctcaaccgcc ggacctacgt catccccgac gacgtcaggg ccctggcccc
     4021 ctcgacgctc cgccaccgcc tcctcctcac ccgggaggcg gctatcaccg gggtcaccgc
     4081 cgaagcgatc atcgccgaca tcgtcgagag cgtcggggtg tcgtaaggat gatccggccg
     4141 acccggacga gcggcggcat agcggccctc gccctcgccc tcgccgtcac ggcgctcctc
     4201 ctcgagaacc cggccgcagc attcgccgcc ggttccctcg ccctcttcct cctctggcgg
     4261 ggctggcggt tcgagagcga ccttgccgcc gcggcgactt cgttagaggt cattcgcacc
     4321 gtcgaccgga cgatcctccg gcaggggaca gcggcgaccg tccgggtaca gatcaacctc
     4381 accgtcccgc ccggcatgga tatccgggtg cgcgacgtac cgccggcggt cgctgtcggc
     4441 gatgcccccc catcaccgcc cggcagggcc gtgacttaca ccatccggct catggcaccc
     4501 ggagaaacgt cttttggagg ggtcgccatc gaggcggccg acaccttctt taccacaaac
     4561 ctcgtatgcc gccggtctgg cgccccgcag atccgggtct ttcccgccgg aacagtcggg
     4621 gacggccggg ggaggggggc aggcagcggc gataccgagg tggaccggcg gatggccatc
     4681 tccggccaga gcatcctcgg gttccggccc taccagacgg gtgacgaccc caacctgatc
     4741 gactggaaga tcactgcccg gcgtgacacc ctctacgtcc ggcagatgac cgggcttgag
     4801 ggcggcgccc ccctcatcgc cgtcgacctc ccgggccggg ccggagaccc ggcagccttc
     4861 gcccggttct cgatggccgt ctccggcgcc gtggaaggcg cgatcagcgc ccgcgacggc
     4921 tgctctcttc tgatcgtcgc cggccccgag atcgtccgat tcctcccgag gacgacggag
     4981 atccgtgaag ccctcgccgc tctcggcggg cttgctcccc tcgagccccg cgcccccctc
     5041 taccgtgcgc cggggccggc cgtccttgcc gcccgggcaa gagtgccccg gcagggtagg
     5101 ggagaagaag agaccgccta cctggcaagg ctctgcggcg ccctctccgc gtttgcaggc
     5161 gagaacccgg cactcttcgc ggcagcggtg cggacggcgt tcagcgtcgc agaggcacga
     5221 gaggtccggg tctactccct cctcccgccg ggcgacagaa gtcacctcat ccagctcgcc
     5281 ggcgaggcga agatccgggg tatgcgggtc gtcctgaagg cccccgccgg caccgggacc
     5341 cttccgggcg tcgacaccgt ggaggtgatc tgatggagat cgatcgaaag ttcctcctcc
     5401 tttgcgcggc cgaggtcctg gtcgtcgcgg tggataacct cattctagcc ctcatcctcc
     5461 aggtgatcgc cctcggcgcg ttcgtcggcg accggcgggg ctacccggtc ttcgtcgccg
     5521 gggcgatcct ctttgccgcg gtcgtcgcga gcgccgggaa cgtcgccctc atcggtcttg
     5581 ccgccgctct cggttgcggc tacctcatcc tcaccctcta cgactaccgg ctcacgcggt
     5641 gggcaggagg cgaggcatga ccacgaaaag tccctaccgg ggagccttca tcctcatcat
     5701 cgccgcagcg gcccttctcg cccttgcggc cctgaccggc aggggggaca tcaccaccgc
     5761 aaccctcgtc ctcgcgggca ccgggtgctt catcgccggt gtcttcctcc tcgccctcca
     5821 caagggcgag cccctcgacc cggatatagc cgccctcctc cccgcccagg ggacgatcgg
     5881 gatcgccacc ctctgcgcgg acctcggcgt ccagggcaac gcctggttcg tcccggagga
     5941 agagaccgtc atcgaggtca taccggtcgc ggataccctc ccggagaaga ccggcgacga
     6001 ctactccttc atcaccagag aaggcgagtg cgccgtccgg ctcgccccgg catgcacccc
     6061 gctcctcgcc cgtctccgcg agaagcatgc cctcgaggtg ccggaggaga acgaagccct
     6121 cctcgcctgc atcaccgagg tctgcgacga cctcctcgag atcgccgaaa agactgaggc
     6181 gaagagagac ggcgagaaca tcgtcgtcac cctctcgggc taccgcctca taccgggctg
     6241 ccggaccatc cgggcggcct cgccgaagtg ctgcaccatg gtcgcctgtc cggtctgcag
     6301 cctcatcgcc gctatagccg cgctcgggac gaagatgccg gtgacgatcg agcacatcga
     6361 cgtcgacgaa aagagaaaga gcctccggat gatcctcacg ccggccggac ccgcacccag
     6421 aggtgcagat cccggtaact cgcgttgatc cggtcctggc ccattatatc ctccccggga
     6481 accgtctcgt tgaagaggag aaactcgatc cggttgtagg cggtcgacgg caccgcaaac
     6541 tcccagggga tctcttctgt ctcgttatgg gggaccgtca ccacgaaccg gtcgagacgc
     6601 tcgaccgcat ggatggtcga cgtgttcgtc gtggtatcga acgtctggtt gagggcgaac
     6661 gcctcgaccg tgtaggggat ctcccggtac tcgtggttcc cgacccctat gatgagcgac
     6721 cgggttgcgc cggccggaaa gtcggtcggg tagtccgcgg ccttcccgcc ggggccgagt
     6781 atgtagaact cggtgaagtg ctccccttct tttgggaccg cgatgacgta ggcggtcgtc
     6841 gcgagcgcgg cgacgatcga taccaggagg agggcggaga gcccccggtc gagccgggag
     6901 gcacccgggt cgaagaactc caggcgagcc gcggcaagca tcgcccgggc cgggacgacg
     6961 aaccggtcgc cggccgggag gaggaggcgc cggtaccagg cggccgcggt catcgcgagg
     7021 gtgaagaggg tgagcgaggc aaggatcggg tcgagccgga tcccccaggg ggtgtagttc
     7081 agcgcaagcc ctatgagagg gacgacggca atagagagcc caaacgagag agccacccgt
     7141 tcgatgccgt cgagatcgcc ccgtgcggga aagagcgccg cgatcagggc gtagccgggt
     7201 atgaagagga ccatcgcgac gccaaagagg atccggagga agctctcgtt taacaccggg
     7261 acgtagacgc aggcaagggt tatggcggtg acggcggcga tcgcgacgag gtcgccggga
     7321 acgtcgccgg cggcctctag gatcaggggt tgtctatctt ctgtatccat gggcggttct
     7381 gtcagcctct tctcttccgc tcaagcatca aggttgcgaa agcgatcacc gggaggaggg
     7441 cggtcagggg ggctgcggtc ggtgtcgtcg tcacggggac ggcggtggtt gtcgcgggga
     7501 tggtcgttgc ggtcggcgcc tccgcaggca ccaggagggc gtaggtaccc gcccgggaga
     7561 tctccgccgt gatccggtcg ccctcgacct tcgagggtac cggcgaccag gcgccgttat
     7621 cgaggcgggc gatcgtcgcc gtcgcgccgg ccgaggggag ggtgaaagag aggaccacgg
     7681 cggggtcgaa cgtccggttt gcgggggtca cggcgtaggc gttcccggca aaggtccacc
     7741 cggcaggggt cgtcccctgc gccgcgacgg caaggaggcc ggcgcccgcg gtatcggtcc
     7801 ccgcaaggga ggcctttccg tcggtcgagg tcaccgtcgc ggtggtgggg gcggtcgtgg
     7861 atacgggccc ggtcgaaccg ccgctgctgc cgcccggtgt gccggaagaa tcgccgttac
     7921 cgctctcggt ggtggtcggt gccggcgtcg acgtcgtcgt cgtcgtcggc gtcgtcggca
     7981 ctcctccgcc gatggtgatc gtccgcgcga gcgcctcgct gccgttgatg tagacggaga
     8041 ccgtcgcggt cccctgctcg atcccttcga gagcaaagga gatcgtgccg tcgatcggcc
     8101 ccttcttcgt gcccatcacc gtgaagtcgg cgatgatggt atcggccgcg gcctccccgc
     8161 ggagggtgac gaggctgatg gttccgctcc cgatgttctg ggattgagag atccggacct
     8221 cgttattcgg atcggcatat ttgagcagga tgacggagcc cccgtcgggg aggttcgccg
     8281 caagctcggt ccagttcgtg cctttcgtgt aggcattcac ctcaccgccg ttgagggcga
     8341 aggggagggt gatcccccgt gccgagaagg caaactcctc gccgggcgtc gccgcgaact
     8401 cgccgcggat gccaagcgaa aaagaggcgc cgtcgggaag gtcgttcacg gtcacggtga
     8461 ccgtatcgcc ggaatcgatc gtatcagggc tgatcgtgat cgtcgccgcc gtcgcggccg
     8521 ggatcagcag aacaagcagg agcaggaatc cacccaatct cttcacgaat aagatctcct
     8581 tgtcgatgaa tacgatatag atgattttcg atactaaaaa agtttatgag gatgtgccgg
     8641 gatccggcgc attacgggga gagcagggat ccggctgccg tgatgaggag gtagagggcg
     8701 aggagtgtct ggaagatgaa gtagccccgg atgtttgcga ccgcgtgggg gagggcgcct
     8761 gcccgcccgg cagaccggtt cgagaggccg cgaagagcga tcgcgagcgg aacaagggag
     8821 aagaccgcca ccggccggaa gtccacgggg gagaggatac ccgtgagcgc aaagagggcg
     8881 aaggccgcgg tcgcgagggc cgccgcggcg gcgatgaggc aaaacccgaa tatccgcccc
     8941 cgccgcacga cgagggtccg ctttcccccg gctatgtcgc cctcccggtc ggggatctcg
     9001 acgctcacga tgaagacgag gccgtagagc gcaagcggta tggcgaacgc gaaaaaggcg
     9061 gggtcaagcc tccccgtcgc gacgaagtag cccgcgccgg gcatcaggag gccgaaggtg
     9121 atcacgttcg cgacctcgcc gaggttgttg taggcgagcg cgaacggcgg cgcggtgtag
     9181 aaccagccga gaaggttccc cgctatgccg aaggcgagga agatcatcgg gtaatcgtag
     9241 accgccacga agagatatcc aatcaggatc gaggccgcca tcagggcgac ggccgcggca
     9301 agcgcggcgg gtttcaggcc ggggtgggcg gcgaggacgc cgctgcctcc cgatatggcc
     9361 gtcgtctcga cgaaccggtc cgcgtcggcg tcgaagtagt cgttgctgta gtggacggag
     9421 aggtgagcgg ccgccatcgc tgcatagccg aagaggaact cccccggggt gaaccgcgcc
     9481 ccgaggagga acccaaagag cgccccggcg gtgaagggta taaagcccga gaggaggaag
     9541 gggaaccgcc cgagccgtat gaatgcgcgg agggcacggg tatccatgga cgactctccc
     9601 gctccgggag aggcgggtcc ggtatataac gttatcctgc cggatcgcac ccggggtccc
     9661 gcaccgcacg ttcgagggcg ccggcgcccc cctggacgta tatgaggccg aggacggccc
     9721 gggcaagggc ctccttctcc gaagagagcg tcttgccggc cgggcggccg accgggctct
     9781 cgtgaagctc ccaccggtcg gagagcgcta cgagaatctc cggcccggag aagcccctca
     9841 gccgcagggt gacgtcgcct atgagggcga gggcccgcgc atcgtcggag aacgccgcaa
     9901 ggcaggcaag gtcccgtgcc gggaccggga gcgcctcctc cggaaacccg gcgagtacac
     9961 cggcaaagag ccgtttcgtg gaggggtcca tcaggacccg gaggaggccg tcaggaccgg
    10021 ggaaggtgtg agcggtatcc cgctcgatgc gggggagggt ctcgcgcgcc acccggccgg
    10081 cctcgaatat caggcgatcg gccgccctgc gccacccccc tatccgccgg agcctcctcg
    10141 ttctccgggg ggagcgccgg tgggcagggc ggcggtagag gcggtactcg cgcgcaaggc
    10201 cgtcccggag cgccgcgagg gtctcctcga tcgagcggtc gagcggaccg cagtcgtagg
    10261 ccatatgatc cagggttggt caaccggacg catttacctt tccccggcag tcaacaacct
    10321 acaggaggag cagaacgacg atcccgacgt gcaagcagag aaagaggggg acgagccgga
    10381 acttcgcctt ctgggtcttg tgccggaaga cgcgcatcgc ggccagggcc ccgagcggcc
    10441 cgaagaacgc gaggagaagg agcctcctct cgggtgtccg ccaggccgcc cgtcccgcca
    10501 gctttttgtc gcggcggaag gcagcaaacg agacggcgtt gacgagtatg taggccgcga
    10561 tcgcggccgg caggaggggc atgccttatc ccccccgccg gatacgctcg gataccccgg
    10621 aatcggcccg taacacttcg accagcagcc ggatctgccg tatcgtctcc ggatggagct
    10681 cgtgctccat gtagcaggcg tcggcgctgg cgatcttctc cgggacgtgg atgatcctca
    10741 ggaactcctt tagcgtatcg tgacgtgcct tgatgacctc ggcgacctcg cgccccgcag
    10801 ggttcaggac caccccctcg tagcgccggt actcgacaaa gcccatctcg tcaagttttc
    10861 ggaacatctc cacaacgctc gacgggctga ggtccagctc acacgccacg tccttggtgc
    10921 gggcgtaccc cttctccagc gtgacgttga ggatggcctc gagatagtcc tcgacccttc
    10981 tgctgaggtt tttcgcacaa cctttgtgtt tcatgcaaca ccagcgcttc atcgtatcac
    11041 agcgcgaaga tccccggttt caaccgggag aggaagcgca ccctccgaag gatttttgcc
    11101 gcccgaagac gatatcgccg agatggtccg ccggtgctcg cgggaatctt ttaccgctgt
    11161 tgcgacccgt cgatgaccca cgatcgagag cagccggcgt atccgtctt
//
LOCUS       DAOSEE010000071        11184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_2229_length_11184_cov_273.956869, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000071 DAOSEE010000000
VERSION     DAOSEE010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 11184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11184
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2229_length_11184_cov_273.956869"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PLI76_06700"
     CDS             <1..254
                     /locus_tag="PLI76_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A''"
                     /protein_id="HOI61756.1"
                     /translation="LVADMMCMDGEVKQIGRHGIAGEKESVLSRAAFEVTVNHLLDAA
                     IANEVDELNGVTENVIVGQPIQLGTGDVKLIAKPVNLKI"
     gene            266..553
                     /locus_tag="PLI76_06705"
     CDS             266..553
                     /locus_tag="PLI76_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30e"
                     /protein_id="HOI61757.1"
                     /translation="MDFNASLRKAVKTGTVFLGQNKTRECIEEGRARLVVVAKNSPES
                     VKNLVNEIDIPVYVYEGSSVQLGKACGMPYVVSALAVVEPGESDILNAARV"
     gene            557..1006
                     /locus_tag="PLI76_06710"
     CDS             557..1006
                     /locus_tag="PLI76_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusA-like transcription termination
                     signal-binding factor"
                     /protein_id="HOI61758.1"
                     /translation="MPQVVLTEECMRLISQFESLTGAGSRDCVVDNRNERIIFVINPG
                     DMGLAIGKSGSSIKKASEVMGKRIEVVEYSTDPAQFLRNCFLPAQVTGVDFDTNEEDQ
                     QVAHIEVRSEDRGLAIGKAGKNIFKAKVLALRQHDIADVVLMQNEAA"
     gene            1257..2696
                     /locus_tag="PLI76_06715"
     CDS             1257..2696
                     /locus_tag="PLI76_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61759.1"
                     /translation="MLILHLLAAAAIAALCLGSIASCAAAAPVPGENDTPGVPPAPGT
                     IAGEKPVTAELPGTAVESPGLAEKPVTARPADIAATLGETPPSDIINATPSLSATATP
                     EPVVSSPDGADGEETPVPTLEDSNVTASPEPTVSTLSTADDVDDEDEPEETPVPVPTL
                     EREAPPRPNATMAKSHQYHDRPLAQGAHPLVRPTSPYETNETVEQPEDVTPREGLFVR
                     GVGVLLTRTPEDVALTRSGVETANLDWLLDTAMRLGGRRPRVAMEGETFTVTVTVEAQ
                     SLAVTGEEPAYVVLVPPPGEVAVYDITRTKEPRNLLDGERAVWEFSVTTHVGRLMLEN
                     LTLPEERLITDLTSYPDAFAFRAYALAGTQEIPSMGLSDPVIAIRSAGSAAAESSLPP
                     LYTLVGVQDSLLDPSETMVDAWRRGIDYDAITAGAGEHLGTLASLGKEEVAAIYTAEG
                     RAGAEAAAGTSSSIIDLIVEFFRSIFGEF"
     gene            complement(2709..3740)
                     /locus_tag="PLI76_06720"
     CDS             complement(2709..3740)
                     /locus_tag="PLI76_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1786 domain-containing protein"
                     /protein_id="HOI61760.1"
                     /translation="MIDLTTPLLAIDVGRGTQDILVYEPGRSIENSIKLVLPSPTVVT
                     ADRIRRATRAGRPIFLEGFLMGGGANTGAVRQHLAAGLPVYATPDAAGTLHDDPERVR
                     SMGVAIETNPPEDAVVVHTTDYMESELRTALSLFGIAYPENVAVAVQDHGYSPHRSNR
                     IHRFELVRERLDAGDWDLLSFVSDPPLAAMTRMQAVRRQAPGALVTDTGPVALIGALC
                     DPEVRRMADKGVILVNAGNGHTLCFALKGREIYGIFEHHTGALTPAKLRHYIRRLADG
                     TLTSEEIFDDGGHGAAVRKPLPTDALAVTGPNRLRLLPEACQAAPFGDMMLSGCFGLM
                     HLWKQFREG"
     gene            complement(3825..8834)
                     /gene="nrdD"
                     /locus_tag="PLI76_06725"
     CDS             complement(3825..8834)
                     /gene="nrdD"
                     /locus_tag="PLI76_06725"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HOI61761.1"
                     /translation="MRLARRSTQTTIFGEFVPTFPKVRTSRGYLLDWDRNRIIRQIVE
                     ETRLVEVFYGYEGADEETAKDIARRVERKIQMLGLQSLSGPLIREIVNMTLLERGLIS
                     YRNVCTRVGTPVFDAHLIDVGRGFEAHDNANLQENAETSHKKKADKISKEQYLLQLPP
                     DLADHHLRGDLHIHDLEYFGTRPFCIDGSTVVPLRMENRIRSLRLDELPIDADTWFPE
                     DLAALTPKGWRRVAKVTRRKVDPGEMRRIRTSGGRSLLVTGEHRIPIKAEDGMAIRRA
                     DEVRPGDALYRISGSDALRGETIESIDLVRELAASVPANLLENVYVRGAEEIFSHVVQ
                     SGQAASYAGISRELGVEHQKQWYTRGIMPVALFSAFSRRYGIEDYAGVTIGVAGSEHE
                     LPAQLTLTPELVRLLGFFVSEGNYNAAPAVGQYNLAITENRQAPAIQAAARAALNTYA
                     TIAGSTPDTTNIYGIEMEHNRGLQVSIGGKAGYLLFRHVFGIPEGAAGKRLPWIVYHL
                     DDVLLHEFLSALFTGDGSAYYRPEKSDCIVNYTTASPALRQELALLLTALGMSPHIAE
                     SSDGETFYRLQLSGRRDIEAFARYTTFFDARQDQIDRFLSAVKERQTAGRGEIVVDVS
                     PAEPTGAYVYDLFIDGDGTEESHTFYASDGLLIHNCQDWDLRYFFYYGLMPDGNGTKA
                     SVAGPAKRAEVAVLHAVKALGSAQTNFAGGQGYYNFLTFMAPFFEGMDYEEIKQLMQM
                     FVYEMTQMMVARGGQVVFSSVQLSPGVPTLWKDKPCVYRGKVWDGEQAPLRTYGEFER
                     EVRLLFKALMEVMLEGDYWGKPFSFPKPEISIEPDFLTENEAYNREHPDLPTYHDLYL
                     MTFELASKFGTPYYDNQIPAYRGAGEGISCYQCLAGDELVPVADSTGRVVVRSIRSLF
                     DVAAKNGRRVDPFGTELAYYDGKTPSVDFTTLEASLRPFRGVMRRRYTGDLLRITLES
                     GRQITVTPDHPVYILNGGGFARETAGDLETGDYLPVLTAEGLGEGSVTGIDLAEVLTK
                     AGHAGAIRVTEDAVTIPGTRHPGLPRTLPVSPELAVFLGYYLAAGSSDHAGRHYTVRL
                     AFGRHDADSAADAAACIRAALGYEPQIQKSTTGIDVVVRSKLIFLLVDALGCGSSAET
                     RSVPDLLFNLDRILAGDYLGAAFLATGKRTIGRRARSIRLKTASEDAARKVVWLAGQL
                     GIQMSYAEREHGIHRPGAPQTTYTCSITAQDQIERFSAATGFPAEMPLGRERGGAFTR
                     LPEGERARGYTALACASKYSAGGVEGVQVSLFLPPITQPAGKGRFAGGDVHPLRVRSV
                     EPVVYDGHVYDLVDVAGTHTFANALGIVTGNCCAYQFSSLADEDDEFEDKLHFREGKH
                     FSMGSWQVMSINCPRAAYKAEGNQERLFAELKALMDTAVGLYRIKRRWMSLIRANGRM
                     PFAMQRPKDPNTGERGAVAVDLEGLVYTIGVVGVNEMVQHFTGHQLHESKEAFRLAVR
                     AMTELEMYARELSQKHNMTIALARTPAETTGQRFAVADLLDERFRDHAIKVVKGDADK
                     ALDMLGKTLDLPIYYTNGTHVTPSAPVPLTKRIEIEHVFFPVVDGGNIFHIWLGEARP
                     DPRGLMEMAMNLCRTTQIGYFAFTRDLTVSLKEYRELKPAHPRPGRAAGFSPAADRAD
                     A"
     gene            complement(8831..9100)
                     /locus_tag="PLI76_06730"
     CDS             complement(8831..9100)
                     /locus_tag="PLI76_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HOI61762.1"
                     /translation="MSDTAQFIVYTLEFCPNCEILKEFLSSRGASFIECDMASAEALT
                     ELRMGGIFVREAPVLQKGEEFYTSADLFAGGAFREDLVADLIAEA"
     gene            9321..9860
                     /gene="thpR"
                     /locus_tag="PLI76_06735"
     CDS             9321..9860
                     /gene="thpR"
                     /locus_tag="PLI76_06735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOI61763.1"
                     /translation="MVRTFVAIDLPEAIRERARASQAALARAEGRLAIVDPANLHVTV
                     KFLGEVDPAQIGPIGEALGAVTADPFDLTVGRAVCNPPRRARVIWCDVIDAGESAAIA
                     RQVDDLLSPLGFSRENRPFRPHVTLARVKEFHPSQCREVASLPPEPLGRCRVASIKLK
                     KSTLTPRGSIYEDLLEVPL"
     gene            9884..>11184
                     /gene="cca"
                     /locus_tag="PLI76_06740"
     CDS             9884..>11184
                     /gene="cca"
                     /locus_tag="PLI76_06740"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOI61764.1"
                     /translation="MLRRIRPTPEERAYIRTIGERLIEAVERSGMAKAMMVGSVARDT
                     FVRGDRDLDVFMLFDPALSREALQEEGLSLAHRVAEEFGATWREKYAEHPYVNATINS
                     LDIDLVPCYAVASATDIKSAVDRTPFHTRYILAHIDGYADDVLLAKQFAKAGGVYGSD
                     HMTEGFSGYLCEILTIYYGGFHALLCAAACWRPGEVVDIEGHGRKPFDEPLVVIDPVD
                     PERNVAAALSLSRMFEFAELARGYLAEPSEAFFCRSPSPPLTRDVFSRLLAARGTHLF
                     SVTFATPDYTPDTVVPQLRKSAESIRELLERSGFAANRIDVCMGKERCVLLFELMASD
                     VPAMRRHIGPPLSSRENAEKFLAKYVQEDVFAGPYIEDGRYVVEVPRPFTRAADLLRS
                     RILFDVALGKHVRRSMKQGWTVKVGAECWDDAFAGFLSGFMG"
BASE COUNT         1905 a         3495 c         3699 g         2085 t
ORIGIN      
        1 tgctcgtcgc ggatatgatg tgcatggacg gcgaggtcaa gcagatcggc cggcacggta
       61 tcgccggcga gaaggagagc gtcctctccc gggctgcatt cgaggtgacg gtcaaccacc
      121 tgctggatgc cgcaatcgca aacgaggtgg atgaattgaa cggcgtcacc gagaacgtga
      181 tcgtgggcca gcccatccag ctcggcaccg gtgatgttaa actcatagca aaacctgtaa
      241 acctgaaaat ctaggagaac catacatgga ctttaatgct tcacttcgta aagccgtgaa
      301 gactggtaca gtattcctcg gccagaacaa gacccgggaa tgcatcgagg agggcagggc
      361 cagacttgtc gtggtggcca aaaacagccc ggaatccgtc aaaaatcttg tgaatgagat
      421 cgatatccct gtttacgtct acgagggctc gagtgtccag ctcgggaaag cctgcggtat
      481 gccgtatgtc gtcagcgcgc ttgccgtggt cgaaccaggt gaatccgata tcctgaatgc
      541 cgcgagagtg tagatgatgc cgcaggtcgt gctgactgag gagtgcatgc gccttatctc
      601 tcagttcgag agcctcaccg gtgcaggcag ccgcgattgt gtcgtcgata accgcaacga
      661 gcggatcatc ttcgtgatca atcccggcga catgggactt gccataggca agagcggatc
      721 gagcatcaag aaggcatccg aggttatggg caagaggatc gaggtcgtgg aatactccac
      781 ggatccggcc cagttcctcc ggaactgttt ccttcccgcc caggtcaccg gcgtcgactt
      841 cgatacgaac gaggaggacc agcaggtcgc ccacatcgag gtgcggagcg aggaccgggg
      901 tcttgccatc ggcaaggcgg ggaagaatat attcaaggca aaggttcttg cactgcgcca
      961 gcacgacatc gccgatgtcg tgttgatgca gaacgaggct gcctgaacct ttctcccctt
     1021 cttttcaatc aacccgcatc cgtaccctcc ctatccggaa tggcgatccc cgactgccgg
     1081 gggtcctctc gcagggctga tccgcacagg aggttccggg cgagccctga tacggaaatt
     1141 atttatactc gtgcgcgctc catggtccaa ccatgagcgg aggatccggg cggatggtgg
     1201 ggcctccggg tagaacccgg ggagagggca gagggagttt gcgaatgccg ggaggggtgc
     1261 tcatcttgca cctcctcgcc gccgccgcga tagcggcgct ctgccttggc tcgatcgcct
     1321 cttgtgcggc tgcagccccg gttcccgggg agaacgatac tcccggagtg ccgccggcac
     1381 ccggcaccat tgccggcgag aaacccgtca ccgcagaact gccgggtact gcggtcgagt
     1441 cgccaggatt ggcagaaaag cctgtaacgg cgaggcctgc ggatatcgcg gccactctgg
     1501 gagagacgcc gccctccgac atcatcaacg cgacaccttc cctgtcggcg acagccactc
     1561 ctgaacctgt tgtttcgtcc cccgatggcg ccgacggtga ggagacgccg gtgccgaccc
     1621 tggaggactc gaatgtgacc gcttctcccg agcctaccgt gtcgaccctc tcgacggccg
     1681 atgacgtcga cgatgaggat gaaccggagg agacgccggt gccggtgccg accctggagc
     1741 gggaggctcc cccgagaccg aatgcgacga tggcgaagtc gcatcagtac catgaccgcc
     1801 cgctggccca gggagctcac ccgctcgtca ggcctacatc gccctatgag acgaatgaga
     1861 ccgtggagca gcctgaggac gttacaccgc gcgaaggcct cttcgtcagg ggcgtcgggg
     1921 ttctcctcac ccggaccccc gaagacgtcg ccctcacccg gagcggggtg gagacagcga
     1981 acctcgactg gctgctcgat acggccatgc gccttggcgg tcggcgccca cgggtggcga
     2041 tggagggcga gacgttcacc gtcacggtga cggtcgaggc gcagagcctg gcggtcacgg
     2101 gagaggaacc ggcgtacgtc gttctggtgc cgccgccggg cgaggttgcc gtctatgaca
     2161 tcacgcggac aaaagagccc cggaacctcc tggatgggga acgggctgtc tgggagttct
     2221 cggtcaccac ccatgtcggc cggctgatgc tcgagaacct cacgctccct gaggagcggc
     2281 tcatcaccga cctgacgtcg tatcccgatg ccttcgcgtt tcgggcctac gctcttgccg
     2341 gcacccagga gatcccctcg atggggctct ccgatccggt gattgccatc cggtctgccg
     2401 gaagtgcggc cgcggagtcc tctcttcctc ctctctacac gcttgtcgga gttcaggact
     2461 cactccttga cccatcggag acgatggtcg atgcgtggcg gcggggcatc gactatgacg
     2521 cgatcaccgc cggggccgga gagcacctcg gcactctcgc gagcctcggg aaggaggagg
     2581 tggcggccat ctacaccgca gaaggaagag ccggggcgga ggcggcggcc ggcacctcgt
     2641 cgtcgataat cgacctgatc gtggagttct tccgcagtat cttcggagag ttttgagggt
     2701 gaaccccctc acccctcccg gaactgcttc cagaggtgca tgagcccgaa gcatcccgag
     2761 agcatcatgt ccccgaaggg tgctgcctgg caggcctccg ggagcagccg gagccggttt
     2821 gggcccgtga cggccagggc atcggtggga aggggttttc tgaccgcagc cccgtggccg
     2881 ccgtcatcaa agatctcttc cgacgtcagc gtgccgtcgg cgagccgccg gatgtagtgc
     2941 cggagtttgg ccggggtgag ggcgccggta tgatgctcga agatcccata gatctcccgt
     3001 cctttcaggg caaagcagag ggtatgcccg ttcccggcgt tgacgaggat cactccttta
     3061 tctgccatac gccggacttc ggggtcgcag agcgccccga tgagcgccac cggcccggta
     3121 tcggtgacga gcgccccggg cgcctggcgc cggacggcct gcatcctggt catggcggcg
     3181 agcggggggt cggagacgaa cgagaggaga tcccagtccc cggcatccag ccgctcccgc
     3241 accagttcga accggtggat gcggttgctc cggtgcgggg agtagccgtg gtcctggacc
     3301 gcgaccgcga cgttctccgg gtacgcgatg ccaaagaggg agagtgccgt ccggagttct
     3361 gactccatgt aatcggtggt atggacgacc accgcatctt ccgggggatt ggtctcgata
     3421 gcgactccca tcgaccggac cctctccgga tcgtcgtgga gggttccggc ggcatcggga
     3481 gtggcgtaga cggggaggcc tgccgccagg tgctgccgta ccgcgccggt gttcgcgccg
     3541 ccgcccatca ggaacccctc gaggaagatc gggcggccgg cccgggtcgc ccggcggatc
     3601 cgatcggccg tcaccaccgt cggagagggg aggaccagtt tgatgctgtt ctcgatagaa
     3661 cggccgggtt cgtagaccag gatatcctgg gtgcccctgc cgacgtcgat cgcaagaagc
     3721 ggtgtcgtca gatcgatcat gagtgagcct cgcacgccgc cgtcctcacg acaggcggcc
     3781 ttaacaggat ctcttttacc gggactaaaa aggtgtgatg ccggtcaggc gtccgcccgg
     3841 tctgccgccg gcgagaaacc ggccgccctc cccgggcggg ggtgggctgg cttcagctcc
     3901 cggtattctt ttaaagagac cgtcaggtcg cgggtgaagg cgaagtagcc gatctgggtc
     3961 gtccggcaga ggttcatcgc catctccatc agcccccggg gatcgggacg cgcctccccg
     4021 agccatatgt ggaagatgtt cccaccgtcc acgaccggga agaagacatg ctcgatctcg
     4081 atccgtttcg tgagcgggac cggggccgac ggggtgacat gggtcccgtt ggtgtagtag
     4141 atggggaggt cgagcgtctt gccgagcata tcgagcgctt tgtcggcgtc gcccttgaca
     4201 accttgatcg cgtggtcccg gaaccgctcg tcgaggaggt cggcgaccgc gaaccgctgg
     4261 ccggtcgtct ccgccggggt gcgggcgagg gcgatcgtca tgttgtgctt ctgcgagagt
     4321 tcccgggcgt acatctcgag ctccgtcatg gcccggacgg cgaggcggaa cgcctccttt
     4381 gactcgtgga gctggtgtcc ggtgaagtgc tgcaccatct cgttgacgcc gaccacgccg
     4441 atggtgtaga cgaggccttc gagatccacg gcgaccgccc cgcgctcgcc ggtgttcgga
     4501 tctttcgggc gctgcatcgc aaacggcatc cgcccgttcg cccggatcag cgacatccac
     4561 cggcgcttga tccggtagag gccgacggcg gtgtccatga gcgccttcag ttcggcaaag
     4621 agccgttcct ggttaccttc cgccttgtac gccgcccgcg ggcagttgat ggacatcacc
     4681 tgccaggaac ccatcgagaa gtgctttccc tctctgaagt ggagtttgtc ctcgaactcg
     4741 tcgtcctcgt cggcaaggga ggagaactgg taggcgcagc agttcccggt cacgatgccg
     4801 agggcgtttg cgaaggtgtg cgtgccggca acgtcgacaa ggtcgtagac atggccatcg
     4861 tagactaccg gctcgaccga gcggacccgg agcgggtgga cgtcaccccc ggcgaaccgg
     4921 cccttcccgg ctggttgggt gataggcggg aggaagagcg agacctggac cccctccacc
     4981 ccgccggcgc tgtacttgct tgcgcaggcg agggccgtgt acccgcgagc acgttcgccc
     5041 tccgggaggc gggtgaaggc cccgcccctc tctctcccga ggggcatctc tgcagggaac
     5101 ccggttgccg cggagaaccg ttcgatctga tcctgggccg tgatgctgca ggtgtaggtc
     5161 gtctgtgggg caccgggccg gtgaatcccg tgttcccgtt cggcgtaact catctggatg
     5221 ccaagctgcc cggcgagcca gaccaccttc cgtgcggcat cctccgaggc cgtcttcagc
     5281 ctgatgctcc tggcgcggcg cccgatggtt cttttcccgg tggcgaggaa ggctgcaccg
     5341 aggtagtcgc cggcgaggat gcggtcgagg ttgaagagca ggtcgggcac cgatcgtgtt
     5401 tcagccgagg aaccgcaccc gagagcgtcg accaggagga agatgagttt actcctgacg
     5461 acaacgtcga tccctgtcgt cgacttctgg atctgtggtt cgtacccgag ggcggcgcgg
     5521 atacaggcgg cggcatccgc tgcgctatcc gcatcatgcc ttccaaacgc gagtcggacc
     5581 gtgtagtgcc gtccggcgtg gtcgctcgac cctgccgcaa gatagtagcc gaggaacacc
     5641 gcgagttcgg ggcttaccgg gagggtgcgg ggcaggccgg ggtggcgggt gcccgggatc
     5701 gtcaccgcat cttcagtgac gcggatcgca ccggcgtggc ctgccttcgt gagaacctct
     5761 gcgagatcga tgcctgtaac gctgccctca ccgagtccct ctgccgtgag caccggcagg
     5821 taatcgccgg tctcgaggtc tcccgccgtc tctctggcaa atccgcctcc gttcaggatg
     5881 tagaccgggt ggtcgggggt gacggttatc tgccggcccg actcaagcgt gatccggagg
     5941 aggtcgccgg tatatcgccg gcgcatcacg ccgcggaacg ggcgaagcga tgcctccagg
     6001 gtcgtgaaat cgacgctcgg cgtcttcccg tcatagtagg caagctccgt gccgaacggg
     6061 tcgacccgac gcccgttctt tgccgcgaca tcaaagaggc tccggatcga tcgcacgacg
     6121 accctgccgg tgctgtcggc caccgggacc agttcgtcgc ccgcaaggca ctggtagcac
     6181 gatatgcctt ctccggctcc ccggtaggct ggaatctggt tgtcgtagta gggagtgccg
     6241 aacttcgacg cgagctcaaa cgtcatcagg tagaggtcgt ggtaggtggg gagatccggg
     6301 tgttcgcgat tatacgcctc gttctcggtg aggaagtcgg gctcgatgct gatctcgggt
     6361 ttcgggaagg agaagggctt gccccagtag tctccctcga gcatcacctc catcagcgcc
     6421 ttgaagagga gccggacctc ccgctcgaac tcgccgtagg tccggagcgg cgcctgctct
     6481 ccgtcccaga ccttgccgcg gtagacgcag ggtttgtcct tccagagggt ggggacgccg
     6541 ggagagagct ggaccgacga gaagaccacc tggccgcccc gggcgaccat catctgggtc
     6601 atctcgtaga cgaacatctg catcagctgc ttgatctcct cgtagtccat cccctcgaag
     6661 aagggcgcca tgaacgtcag gaagttgtag tagccctggc cgccggcgaa gttcgtctgc
     6721 gcggagccga gcgccttgac ggcgtggagg acggccacct cggccctctt tgccggtccg
     6781 gcgaccgacg ctttcgtccc gttgccgtcc ggcatcaggc cgtagtagaa gaagtagcgg
     6841 aggtcccagt cctggcagtt gtggatgagg aggccgtcgg aggcgtagaa cgtgtggctc
     6901 tcctcggtcc cgtcgccgtc gatgaagagg tcgtagacgt acgcaccggt gggctcagcc
     6961 ggactgacat cgacgacgat ctctccccgt ccggcggttt gcctctcttt cactgcggag
     7021 aggaacctgt cgatctggtc ctgccgcgca tcaaagaagg tggtatatcg tgcgaatgcc
     7081 tcgatatctc ttctcccgct gagctggagc cggtaaaatg tctccccatc agaggattcg
     7141 gcgatgtgcg gcgacatacc gagcgcggtg agcaggagag cgagttcctg ccggagcgcc
     7201 ggcgacgcgg tggtatagtt gacgatgcag tccgattttt ccgggcggta gtatgcggag
     7261 ccgtcgcccg tgaagagggc ggagaggaac tcgtggagga ggacgtcgtc cagatggtag
     7321 acgatccagg gaaggcgttt tcctgccgct ccctccggga tgccgaagac atggcggaag
     7381 aggaggtagc ctgccttccc gccgatggag acctgcaggc cccggttgtg ttccatctcg
     7441 atgccgtaga tgttcgtggt atcgggagtg cttccggcga tcgttgcgta ggtgttcagg
     7501 gcggcgcgtg ctgccgcctg gatggcgggc gcctgacggt tctcggttat cgcgaggttg
     7561 tactgaccga ccgccggggc ggcgttgtag ttcccctccg agacgaagaa cccgaggagc
     7621 cggacgagtt ccggcgtaag ggtgagttgt gcaggcagtt cgtgttcgct ccccgcgacc
     7681 ccgatggtga ccccggcgta gtcttcgatg ccgtagcggc gtgaaaaggc gctaaagaga
     7741 gcaaccggca tgatcccgcg cgtataccac tgcttctggt gctcgacgcc gagttcccgc
     7801 gagatcccgg cgtacgaagc ggcctgcccg ctctgcacca catggctaaa gatctcctct
     7861 gcaccgcgga cgtagacgtt ctcgaggagg ttggccggca ccgacgccgc gagttcgcgg
     7921 acgagatcga tcgattcgat cgtctccccg cggagggcgt cgctcccgct gatccggtag
     7981 agggcgtccc cgggccggac ctcgtcggcg cggcggatgg ccatgccgtc ctctgccttg
     8041 atcgggatcc ggtgctcgcc ggtgaccagg agcgaccggc cgcccgatgt ccgaatccgg
     8101 cgcatctcgc cggggtccac cttccggcgg gtcacctttg caacccggcg ccagcccttc
     8161 ggcgtgagcg cggcgagatc ctccgggaac cacgtatcgg cgtcgatcgg gagttcgtca
     8221 aggcgaagac tccggatccg gttctccatc cgcaggggca cgaccgtgct cccgtcgatg
     8281 cagaacgggc gcgtcccgaa gtattccagg tcgtggatgt ggaggtcgcc gcggaggtgg
     8341 tggtccgcaa gatcgggggg gagctgcagg aggtactgct ccttgctgat cttgtccgcc
     8401 ttctttttgt gcgacgtctc ggcattctcc tggaggttcg cgttgtcgtg agcctcaaat
     8461 ccccgcccga cgtcgatgag gtgcgcgtca aagaccgggg ttcctacccg ggtgcagacg
     8521 ttgcggtagg atatgaggcc gcgctcaagg agcgtcatgt tgacgatctc gcggatcagg
     8581 gggccggaga gcgactgcag cccgagcatc tggatttttc tctcgacccg gcgggcgata
     8641 tccttcgccg tctcctcgtc ggctccctcg tagccgtaga agacctcgac gaggcgggtc
     8701 tcttcaacga tctgccggat gatccggttc ctgtcccagt ccaggaggta gccccgcgat
     8761 gtcctgactt tcgggaaggt ggggacgaac tccccgaata tagtcgtctg ggtcgacctt
     8821 cgcgccaacc tcatgcctcc gcgatcaggt cggcgacgag gtcttcccgg aacgcgccgc
     8881 cggcaaagag gtcggccgat gtgtagaact cttcgccctt ctgcagcacc ggcgcctccc
     8941 ggacgaatat cccgcccatg cgaagttcgg tcagtgcctc ggcggacgcc atgtcgcact
     9001 cgatgaagga agcacccctg gaggagagga actctttgag gatctcgcag tttgggcaga
     9061 attccagtgt atagactata aattgcgctg tatcggaaat ggtaataccc ccggagtcgg
     9121 tcgttatgtt ttcgttctgt acggatataa caggtacctt tcttccgaac aggcctctct
     9181 ctcccggtga gcgtgtgtcc cgggcgttac gggggatcgc cgggaactca cccctgcgcc
     9241 gctcgatatc ccggctccgt cgggtacgct cctgtcgtac aatcaccatt ttgtagggag
     9301 aggcacgata gtacggtagt atggtcagga cgttcgttgc aatcgacctg cccgaagcga
     9361 tccgcgagag ggcgcgtgcg tcccaggcgg ctctggcacg ggccgaagga aggctcgcca
     9421 tcgtcgaccc cgcaaacctt cacgtcacgg tgaagttcct cggagaggtc gatcctgcac
     9481 agattgggcc tatcggcgag gcgctcgggg ccgtcacggc cgaccctttc gacctgacgg
     9541 tcgggcgtgc ggtctgcaac cccccgcgga gggcgcgggt gatctggtgc gacgtcatcg
     9601 acgccggaga gagcgccgct attgcccggc aggtggacga cctcctctcg ccgctcgggt
     9661 tttcccggga gaaccgccct tttcgccccc acgtcaccct tgcgcgggtc aaggagttcc
     9721 acccctcgca gtgccgggag gtagcttccc tgccccctga accgctcggg cggtgccgcg
     9781 tcgcgagcat caagctcaag aagagcacgc tcaccccccg ggggtccatc tacgaggatc
     9841 ttctggaggt cccgctttga cccggtgccc ctgtgaggag gaagtgctcc ggcggatccg
     9901 cccgacaccc gaggagcggg catacatccg tacgatagga gagcgcctga tcgaggcggt
     9961 ggagcggtca ggtatggcaa aggcgatgat ggtgggctcg gtcgcccgcg acaccttcgt
    10021 ccggggcgac cgggacctcg atgtcttcat gctctttgac cccgccctct cccgcgaggc
    10081 cctccaggag gaaggtctct ccctcgcgca ccgggtcgcg gaggagttcg gcgcgacctg
    10141 gcgcgagaag tatgccgagc acccctacgt caacgcgacg atcaactcgc tcgacatcga
    10201 cctcgttccc tgctacgccg tcgcaagtgc caccgatatc aagagcgccg tggaccggac
    10261 accgtttcac acccgctaca tcctcgccca catcgacggc tacgccgacg acgtcctttt
    10321 agccaagcag tttgcaaagg cgggcggggt ctacgggtcg gaccacatga ccgaggggtt
    10381 ctccggctac ctctgcgaga tcctgacgat ctactacggc ggtttccatg cgctcctctg
    10441 cgctgccgcc tgctggagac ccggggaggt ggtcgatatc gaggggcacg ggagaaagcc
    10501 gtttgacgaa ccgctcgtcg tcatcgatcc ggtcgacccg gagaggaacg tggcggcggc
    10561 gctctcgctc tcgcgtatgt ttgagttcgc tgaactcgcc cgcggctacc ttgccgaacc
    10621 gtcggaggcg ttcttctgcc ggtcgccttc tccgcccctc acccgcgatg tcttttcccg
    10681 cctgctcgca gcacggggga cgcacctctt ctccgtcacg tttgcaacgc cggattacac
    10741 ccccgacacg gtagtccccc agctccggaa gtccgccgag tcgatccgcg aactcctgga
    10801 gcggagcggg tttgcggcaa accggattga cgtctgcatg gggaaggagc ggtgcgtcct
    10861 cctcttcgag ctgatggcga gcgatgtccc ggcgatgcgc cggcatatcg ggccgccgct
    10921 ctcgtcgaga gagaacgcgg agaagttcct cgcaaagtac gtgcaggagg atgtctttgc
    10981 cggcccctac atcgaggacg gccgctacgt cgtggaggtg ccccgcccct tcacccgtgc
    11041 cgccgatctc ctgcgttcca ggatcctctt tgatgtcgcg ctcggcaagc acgtccgtcg
    11101 ctcgatgaaa cagggctgga cggtgaaggt gggtgcggag tgctgggacg atgcgtttgc
    11161 cggattcctt tcggggttca tggg
//
LOCUS       DAOSEE010000072        11079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_2260_length_11079_cov_256.894775, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000072 DAOSEE010000000
VERSION     DAOSEE010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 11079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11079
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2260_length_11079_cov_256.894775"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1796)
                     /locus_tag="PLI76_06745"
     CDS             complement(<1..1796)
                     /locus_tag="PLI76_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOI61765.1"
                     /translation="MRAHEDEELGLIAAVRNLLTQSGGPGSGNGDTLAEPQGTVPAPW
                     PDEVEIRGETDPDPGETDDTAAFPPVAAPESEDTRILARPGHTGDRRAADREDPIRAL
                     LDRIERRRMGEEAASPPSPVAEETGTTPAPFGILGRMRGRKDAGAPPLDEPEPVDDAG
                     APPLDGPDPVDDAGVISVEELGNLADLILPKSATFTVDELSINHHEHRFDFVDNARVV
                     SEFDDLFARAFSTTTFTAAEIAVPAEPEVQSRFGFLKKFYPTAGEIIEEYNLTLHGPL
                     VDLSLRPTPGVEEIELYPVNEPYAYVRVTYDSTTHEYTYHVLEPVLTPGEQELLSEIK
                     ERLFETLNIATRDLTRDAAKKALRGAANTIVADYGIRLPPPGREKILYHVEKEFLGDG
                     LIDPVMHDKYIEDISCDGVGSPIFVYHTTYESMRTSLVYRNAGELDSFVTKLAQRAGK
                     YISIAEPMLDATMTDGSRIQMTLGSEVTAHGSTFTIRKFRDEPITPTDLIEWHTFSPL
                     GIAFLWLAVENAKSCIFAGGTASGKTTTLNAISLFIPPLAKIVTLEDTRELKLPHPNW
                     IPSITRDSFSQDGRGEIDMYELLRAALRQRPE"
     gene            complement(1783..2403)
                     /locus_tag="PLI76_06750"
     CDS             complement(1783..2403)
                     /locus_tag="PLI76_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61766.1"
                     /translation="MNEILLQSTYEGCFTLDELLHHGSVHSLTGIGASKEQTQAFFLV
                     LLGGEPDGAIFIDEKGTLFGDTAVLHLTGQEEFNLHRVAPPIADALVARCRVFEKSHL
                     KKSGRLEIPTIGAPPRQRVGVLCLTVQDNRVPLAGAHVSIRKGKLVITGDVTDSEGRV
                     CFRLLAGRYMCVVTDRTGERTRCIIEFHEPQVETFVDIGGTEDEST"
     gene            2755..3549
                     /locus_tag="PLI76_06755"
     CDS             2755..3549
                     /locus_tag="PLI76_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HOI61767.1"
                     /translation="MILASIPLMIAAAAIGVTLIYASVLDARERRVPHRTWRPALAIA
                     VPAAAIVYGLATLADWQAAAGYLVLVVVFCGFFYLFAARNLFGGADAYALIIITACIP
                     FFPVEPYFGIPPLGFFPFTVLTNAVLINIAAPVAILAKNLVEGNRAPFPCLFLGFPVD
                     GKTIQNEFGFVMEDIEEEDGRLVRRFVGFAGSLGRMVRGEQRIYTKDLRLHPEDYERE
                     LALYRKAGRVWISYGIPFIIPIAAGFLTALFIGDILFVIMATFAGV"
     gene            3556..3924
                     /gene="hisI"
                     /locus_tag="PLI76_06760"
     CDS             3556..3924
                     /gene="hisI"
                     /locus_tag="PLI76_06760"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HOI61768.1"
                     /translation="MEIQFNDNGLVPVIVQERRTRDVLMLAYANALALELTRTTGYAH
                     YYSRSRQKLWKKGEESGHFQRVCRILVDCDADAVLYEVEQVGAACHTGHASCFYRTAD
                     GEEIAGMVFDPKKVYANKGE"
     gene            3948..5843
                     /locus_tag="PLI76_06765"
     CDS             3948..5843
                     /locus_tag="PLI76_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PINc/VapC family ATPase"
                     /protein_id="HOI61769.1"
                     /translation="MKIVPDTSVVIDGRITSLIKTGEYKGATIIIPEAVIAELEAQAN
                     QGREIGFSGLTELQELFRMSVDGIIELQFSGERPSLDQVKLSSGGEIDALIRNVAIVY
                     GARFITSDLVQAEVAKAKGLDVVYLRPQIGDFSPLSIDQFFDEETIAITLKERALPVA
                     KVGGLRDTRLVTLRDTPMSEYELRSMAQELLERAKRDPDGFIELEKRGITVVQIGSLR
                     ISIARRPFADGMEITVVRPLVDLSLDDYDMAPEIRQRILGNRRGVLIAGPPGAGKTTL
                     AQGLATFLADHDFIVKTMEAPRDLQVPDHITQYMALEGSMANTAEALLLVRPDYVIFD
                     EIRKNEDFNVYADMRLAGMGMVGVVHAMEVHDCLQRFCDRVDYSILPQIINTVIFVVQ
                     GAITKMYDLELTIKAPEGMPGETHVRPVIVVRDHARQETEIEIFRYEGETLVMPLTRV
                     AVEPPAVLEPAPAVAAAAAAPEPGENVAWKVAEKEVQREIGRYTSGPVDVRIISDNKA
                     VVYIEDKDVPAAIGKGGKNVSAIVNKLGIGIDIRPHSELEAKKPVEEEMQLAGGIRIR
                     IDKKQLTIISPENKGKIVDVFAGKEYLFTATVNEEGDILLAKNSTIAQEMIKRYNEGE
                     TIRLRPV"
     gene            5901..8666
                     /gene="leuS"
                     /locus_tag="PLI76_06770"
     CDS             5901..8666
                     /gene="leuS"
                     /locus_tag="PLI76_06770"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOI61770.1"
                     /translation="MQSNERECIARWEHAFEADPEREKEKYYLTVAYPYPSGAMHVGH
                     GRTYIVPDVLARYQRMAGRQVLFPMAFHVTGTPVIGISKRIANGDEKTVGLYRDLYRV
                     PQDILDRFINPMEIVRYFSEEYRRVMQKSGLSIDWRRRFITVDPPYSKFIEWQYKHLR
                     EDEHVVRGAHPVKYCPQCENPVGDHDLLEGDKAEIIRFTLVVFRWGNARVPCATLRPE
                     TIYGVTNLWVNPAVTYVRAMIDGKEWIVSSEAAAKLSLQDHAVTVTEKIPGTALVDQT
                     VTHPLSGDVPVLPATFVDPDMGTGIVMSVPAHAPFDYIALRDLQQQGRYTSVEPIPLI
                     AVEGYGDIPAKDAVERAGIHDQNDPGMEALTQEVYSAEFSRGKVFEKYGGKPVREARD
                     DVAALMMERYDSVPMYDFDNRLVICRCGGRVFVKILHDQWFLEYSDPCWKEQVKTQLE
                     EMALVPPEVRAEFIRTVDWLKDWACTRRVGLGTKLPWDPTWIIEPLSDSTIYMAYYTI
                     AHHLKAIPPEKLTPAVFDYIFFGMGSPETVDRETLDAIRSEFLYWYPYDYRFSAKDLI
                     SNHLTFQLFHHRAIFPSYLQPKGMVVFGMGLLNGAKMSSSKGNVFLLEDALEEFGADT
                     VRMFLVGSAEPWQDFDWRNELVSSTKRQIERFWNTVSEAMQAEGATPIDAWLASRLER
                     RIEAATAALDSFQTRQALQEAFFGVESDLKWYRRRLPEGAGGGAVLQDLCRTWVRLLA
                     PFIPFTCEALWSDLGESGMVSFAPWPETDESRINPGVELAEELLARTVEDIESIVKLI
                     PMEPASISLFVAPAWKYEAFRIIAGSADKTRVMREIMQNESMRRRGREATDAAKQITK
                     LVLKLPPDIVKQLAASPLDERAVLEGARAFLEHEFGVPVTVKDAGESNHPKAAAAMPF
                     KPAIMIE"
     gene            8787..9152
                     /locus_tag="PLI76_06775"
     CDS             8787..9152
                     /locus_tag="PLI76_06775"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HOI61771.1"
                     /translation="MPMLLFDGEFVGTREQDRRLSGLFAAAEYRQERMRVIESRCGIL
                     CSGCGYRAEMGCGGCMAIKRPFWGEICPLKACCEARGLENCGRCGEFPCEVLVQFAFD
                     ERQGDGGRRIEQCRAWSLQ"
     gene            9128..9517
                     /locus_tag="PLI76_06780"
     CDS             9128..9517
                     /locus_tag="PLI76_06780"
                     /inference="COORDINATES: protein motif:HMM:NF016854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HOI61772.1"
                     /translation="MQGVVSAVEAIMMASSSEFVEYVAGQLAGAGDITYRKMFGEYGI
                     YCDGKFFACACDNQFLVKITEAGKKFMPDGRTASPYPGAKPCFLIDDLDDREFLRELT
                     AITCAALPAPRPKKRRAGTAARRQGED"
     gene            complement(9569..9895)
                     /locus_tag="PLI76_06785"
     CDS             complement(9569..9895)
                     /locus_tag="PLI76_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5611 family protein"
                     /protein_id="HOI61773.1"
                     /translation="MQEYQIKRGYTKDLAKSMVQGLRDQFGLEPEVAADGHYIVAYGA
                     LHRLEVWSGAGGKTLVVDTEANRDAGDEAIIDTNRRFRNYLQQVTGYTAKERAKKAQQ
                     ALKGSE"
     gene            9955..10668
                     /locus_tag="PLI76_06790"
     CDS             9955..10668
                     /locus_tag="PLI76_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome assembly chaperone family protein"
                     /protein_id="HOI61774.1"
                     /translation="MDDIKVISRALAGAEKTVLIGFPGSGLVGSIALQYLVEQMDFEQ
                     IGAITSKYFPPVALMTRGVINAPVRLYEKDSLAAIISDVPVHPMICYEVANGIMDWLT
                     QFGIREIATIAGIITNEPDKRVFGVATTSTVLQRIEEQTIILPMGSISGIAASILTEC
                     KTRGIPGIGLLGETVNTPDPRSSAATIEVLNKIYDLDLDVQPLLEQAVEIEAAMAQIA
                     EQVQKTEAQPRREQLPMYG"
     gene            10675..11064
                     /locus_tag="PLI76_06795"
     CDS             10675..11064
                     /locus_tag="PLI76_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF473 domain-containing protein"
                     /protein_id="HOI61775.1"
                     /translation="MKCAALTGISPSVIAELKTGRARTLELNSAHNIITLTEAAPGEC
                     IFMTSVNGEDLSPGDSGIMVDLLALTISMKRVEVVNPLYYEERERMSARVKVQFRGTT
                     IARGVEERKWGDPTMVEIIRSACYRAG"
BASE COUNT         2186 a         3349 c         3510 g         2034 t
ORIGIN      
        1 tactcggggc gctgccggag ggcggcacgc aggagctcgt acatatcgat ctcgccccgc
       61 ccgtcctgcg agaacgagtc gcgggtgatg ctcgggatcc agttcgggtg aggaagtttc
      121 agctcgcggg tatcctcaag cgtcacgatc tttgcaagcg gcgggatgaa gagcgatatg
      181 gcgtttaagg tcgtcgtctt ccctgaagcc gtcccgccgg caaatatgca ggatttggcg
      241 ttctcgaccg caagccagag gaaggctatc ccgagcggcg agaaggtgtg ccactcgatg
      301 agatctgtgg gcgtgatcgg ttcatcgcgg aacttgcgga tcgtgaacgt cgaaccgtga
      361 gcggtcacct ccgagccgag cgtcatctgg atgcgcgacc cgtcggtcat cgtggcatcg
      421 agcatcggtt cggcgatcga tatgtacttt ccggccctct gcgcgagttt cgtgacgaac
      481 gagtcgagtt ccccggcgtt ccggtaaacg aggctcgtcc tcatcgattc gtaggtcgtg
      541 tggtagacga atatcgggct tcccacgccg tcgcaggata tatcctcgat atacttgtcg
      601 tgcatcaccg ggtcgatcag gccgtcgccg aggaactcct tctccacgtg atagaggatc
      661 ttctcccgcc ccggcggggg aaggcggatc ccgtaatcgg cgacgatcgt gtttgccgca
      721 ccccggagcg cctttttcgc cgcatcgcgg gtgagatcgc gggtagcgat gttgagcgtc
      781 tcaaagaggc gttctttgat ctccgagagg agttcttgct ctccgggcgt gagcacgggc
      841 tcaaggacgt ggtaggtgta ctcgtgggtc gtgctgtcgt aggtcacccg gacgtaggca
      901 tacggctcgt tcaccggata gagctcgatc tcctcgactc ccggtgtcgg ccgcagagag
      961 agatcgacga gcggcccgtg gagcgtgagg ttgtattcct cgatgatctc cccggcagtc
     1021 gggtagaact ttttgaggaa tccgaaccgg gactgtacct ccggttctgc cgggaccgcg
     1081 atctctgccg cggtgaacgt ggtggtggag aacgccctgg caaagaggtc gtcgaactcc
     1141 gatacgaccc gggcgttatc gacgaagtca aaccggtgct cgtggtggtt gatgctcagc
     1201 tcatcgaccg tgaacgtcgc gctcttcggg aggatcaggt ccgccaggtt cccgagctcc
     1261 tcgaccgaga tcacgccggc atcatcgacc gggtcgggcc cgtcgagggg aggtgcgccg
     1321 gcatcatcga ccggttcggg ctcgtcgagg ggaggcgccc cggcatcctt tcgcccccgc
     1381 atgcggccga ggataccgaa gggtgccggc gtggttccgg tctcttccgc caccggcgat
     1441 ggcggggatg cagcctcttc tcccatcctg cgccgctcta tccggtcaag gagcgccctg
     1501 atcggatcct ccctgtcggc cgcacgccga tccccggtat gcccgggtcg ggcgagaatg
     1561 cgggtatcct cgctctctgg cgccgctaca gggggaaacg ccgccgtatc atcggtctcg
     1621 ccgggatccg ggtccgtttc gccccggatc tccacctcat cgggccacgg tgccggaaca
     1681 gtgccctgcg gctccgccag ggtatctccg ttgccggaac ccgggccccc ggattgtgta
     1741 agaagattcc tgactgctgc tatcaatcca agttcttcat cctcatgtgc tctcatcctc
     1801 tgtgccccca atatcaacaa acgtttcaac ctgaggctca tggaactcga tgatgcaccg
     1861 tgtccgctcg ccggtccgat ccgtgaccac gcacatatag cgcccggcga gcagccggaa
     1921 acagacccgc ccttcggagt ccgtcacatc gcctgtgatg acaagtttcc ccttccggat
     1981 agagacatgg gccccggcaa ggggtacccg gttgtcctgt acggtaaggc agagcacccc
     2041 cacccgctgg cggggagggg caccgatcgt cgggatctcg aggcggccgc tcttcttcaa
     2101 gtggctcttc tcaaagaccc tgcaccgtgc caccagtgca tcggcgatcg gcggcgccac
     2161 ccggtggagg ttgaactcct cctgcccggt caggtgcagg accgccgtat caccaaagag
     2221 cgttcccttc tcgtcgataa aaatagcgcc gtccggttcg cccccaagga gaaccaggaa
     2281 gaacgcctgc gtctgctctt ttgaagcccc tattccggtc agggagtgaa cgctaccgtg
     2341 gtgcagcaac tcgtcaagag taaaacatcc ttcgtacgtc gattgcagaa ggatctcgtt
     2401 catcaggccg tttatgtcca tgcagatctt caggtttgat attcagtggt ttttatgtcg
     2461 gagaggggat tttaccccct gccacaatat acctcattac gatgctatta ttaatacctt
     2521 gtgatggtcc gataccggtt ccggcggacg actcgatgaa agtattcgat cagaaaaccc
     2581 tgatgcggcc atatacgggc gagaactctg cgaacgctcc tgaatcgcgg ccaaaagagc
     2641 ggtgatcccg gccccggaga gaccgccggc ggtcttgccg ccgtcccggg agggtccgcc
     2701 cggcggaaag aatcgccaga aataagatat gcaaggtgga tatcgtacct gtccatgatc
     2761 ctcgcatcca tcccgctcat gattgcagcg gccgcgatcg gagtcacgct catatacgcg
     2821 tcggtcctcg acgccagaga gcggcgggtg ccccacagga cctggcgccc cgcgcttgcc
     2881 atcgccgtcc cggcagcagc catcgtatac gggcttgcca ccctcgcaga ctggcaggcg
     2941 gcagccggct acctcgtcct ggtcgtggtc ttctgcggtt tcttctacct ctttgcggca
     3001 cgaaacctct ttgggggggc ggacgcgtat gctctcatca tcatcaccgc gtgcataccg
     3061 ttcttcccgg tcgagccgta cttcggcatc ccgccccttg ggttctttcc gttcaccgtc
     3121 ctgacgaacg ccgtgctcat caacatcgcc gcacccgttg caatacttgc aaagaacctc
     3181 gtcgagggga accgtgcccc gttcccctgc ctcttcctcg gctttcccgt cgacgggaag
     3241 acgatccaga acgagttcgg ctttgtcatg gaagatatcg aggaagagga cggcaggctg
     3301 gtcaggcggt ttgtcgggtt tgccgggtcg ctcgggcgca tggtgcgggg ggagcagcgc
     3361 atctacacaa aagacctcag gctgcacccg gaagattacg agagagaact cgctctctac
     3421 cggaaggccg gcagggtctg gatatcctac ggcatcccgt tcatcatacc gatcgcggcg
     3481 ggattcctga cggcgttatt cataggagat atcctcttcg tcatcatggc gacttttgca
     3541 ggagtgtgaa tcaatatgga gatacaattc aacgataacg gattggtgcc tgtcatcgtg
     3601 caggagagac ggacccgcga tgtcctgatg ctcgcctacg caaacgctct ggcactggaa
     3661 ctcacccgga ccaccggata cgcacattac tatagccgga gccggcagaa gctctggaag
     3721 aaaggagagg agagcggaca tttccagcga gtctgccgca tactcgtcga ctgcgacgca
     3781 gacgcagtcc tctatgaggt ggaacaggtc ggtgccgcct gccataccgg ccatgcctcg
     3841 tgcttctacc ggacggcgga tggcgaagag atcgccggaa tggtcttcga tccgaagaag
     3901 gtatacgcta ataagggtga atgacctcat tactatggtg attttttatg aaaattgtac
     3961 ccgatacaag cgtcgtgata gatggacgca taacatctct gataaaaacg ggagaatata
     4021 aaggcgcaac aataatcata ccggaagccg tcatcgccga actggaagca caggcgaacc
     4081 aggggcggga gatagggttc tccggcctga ccgaactcca ggagctcttc aggatgtcgg
     4141 tggacggcat catcgagctc cagttctccg gagagcgccc gagcctcgac caggtgaaac
     4201 tctcaagcgg cggcgagatc gacgccctta tacggaatgt cgctatcgta tacggcgccc
     4261 ggttcatcac gagcgacctc gtgcaggccg aggtggcaaa ggcaaaaggg cttgacgtcg
     4321 tctacctgcg gccgcagata ggcgatttct cgccgctctc gatcgaccag ttcttcgatg
     4381 aggagacgat cgcgatcacc ttgaaagagc gggccctgcc ggttgcaaag gtaggggggc
     4441 ttcgcgacac acggctcgtc accctccgcg atacgccgat gagcgagtac gagctccggt
     4501 caatggcgca ggaactcctt gagagggcaa aacgcgaccc ggacgggttc atcgaactcg
     4561 agaagcgggg gatcacggtc gtccagatag gatcgctccg gatatcgatc gcccggcggc
     4621 cgtttgcgga cgggatggag atcacggtgg tgcggccgct tgtcgacctc tcgctcgacg
     4681 actacgacat ggcgccggag atccggcaga ggatcctcgg gaaccgccgc ggcgtcctga
     4741 ttgccggccc cccgggtgcg gggaagacca cgcttgccca gggcctcgcg acgttccttg
     4801 ccgaccacga cttcatcgtg aagacgatgg aggcgccgcg ggacctgcag gtgcccgatc
     4861 acatcaccca gtacatggcg ctcgagggga gcatggcaaa caccgccgaa gccctcctgc
     4921 tcgtccggcc cgactacgtg atattcgatg agatccggaa gaacgaagac ttcaacgtct
     4981 acgccgacat gcgccttgcc ggtatgggca tggtcggcgt ggtgcacgcg atggaggtgc
     5041 acgactgcct tcagcggttc tgcgaccgcg tggactacag catcctgccc cagatcatca
     5101 acaccgtcat attcgtcgtc cagggtgcga tcacgaagat gtacgacctt gaactgacga
     5161 tcaaggcgcc ggagggcatg cccggcgaga cgcacgtccg cccggtgatc gtcgtccgcg
     5221 accacgcccg gcaggagacc gagatcgaga tcttccgtta cgagggcgag accctggtga
     5281 tgccgctcac ccgggtcgcc gttgaaccgc ccgccgttct cgaaccggcg ccggccgtcg
     5341 ccgccgccgc ggcagcaccc gaacccgggg agaacgtcgc ctggaaggtt gccgagaaag
     5401 aggtccagcg agagatcggg cgctacacga gcggcccggt cgatgtccgg atcatcagcg
     5461 acaacaaagc cgtcgtctac atcgaggata aggatgtccc ggcggcgatc gggaagggcg
     5521 gcaagaacgt ctcggcgatc gtgaacaaac tcggcatcgg gatcgatatc cggccgcaca
     5581 gcgagcttga ggcgaagaaa cccgtcgaag aggagatgca gcttgccggc ggcatcagga
     5641 tacgcatcga caagaaacag ctgaccatca tctcaccgga gaacaagggc aagatcgtcg
     5701 atgtctttgc cgggaaagag tacctcttca cggcaacggt gaacgaggag ggggatatcc
     5761 tccttgcgaa gaacagcacg atcgcacagg agatgatcaa acgctacaac gaaggggaga
     5821 cgatccggct gcggccggta tgaggggaac aatatgacga gtgatccaga ttttggaggc
     5881 atagcgtgag cggactggat atgcagagca acgagcggga gtgtatcgcc cggtgggagc
     5941 atgcgttcga ggccgacccg gagagagaga aggagaagta ctacctgacc gtagcatacc
     6001 cgtacccgag cggggcgatg cacgtcgggc acgggaggac ctacatcgtc ccggacgtcc
     6061 tcgcccgcta ccagcggatg gcggggaggc aggtcctctt cccgatggcg ttccacgtca
     6121 ccggcacgcc ggtcatcggg atctcaaaga ggattgcaaa cggtgacgag aagaccgtcg
     6181 ggctctaccg cgacctctac cgggtgccgc aggacatcct cgatcggttc atcaacccga
     6241 tggagatcgt ccgctacttc tcggaggagt accggcgggt gatgcagaag tcggggctct
     6301 cgatcgactg gcggcgccgg ttcatcaccg tcgacccccc gtacagcaag ttcatcgagt
     6361 ggcagtataa gcacctgcgg gaggacgagc acgtcgtcag gggcgcccac ccggtcaagt
     6421 actgtcccca gtgcgagaac cctgtcggcg accacgacct cctcgagggc gacaaggccg
     6481 agatcatccg gttcaccctg gtggtgttcc ggtggggcaa cgcccgggtc ccgtgcgcca
     6541 ccctccggcc cgagacgatc tacggcgtga cgaacctctg ggtgaatccg gccgtcacct
     6601 acgtgcgggc gatgatcgac ggcaaggagt ggatcgtaag ctctgaggcg gcggcaaaac
     6661 tctccctgca ggatcacgcc gtcaccgtga cggagaagat ccccgggacc gcgctcgtcg
     6721 accagacggt tacccacccg ctctccggcg acgtccccgt cctcccggcg acgttcgtgg
     6781 atccggatat ggggaccggg atcgtcatga gcgttcccgc ccatgcgccg tttgattaca
     6841 tcgctctccg cgacctgcag cagcaggggc ggtatacgtc cgtagagccc ataccgctca
     6901 ttgccgtcga gggttacggc gatataccgg caaaggatgc ggtcgagcgt gcgggcatcc
     6961 acgaccagaa cgaccccggg atggaggcgc tcacccagga ggtctacagc gctgagttct
     7021 cccgcgggaa ggtctttgag aagtacggcg gcaaaccggt gcgggaggcc cgcgacgacg
     7081 tggcggccct gatgatggag cgctacgact cggtcccgat gtacgacttc gacaaccgcc
     7141 tggtgatctg ccggtgcggc ggcagggtct ttgtaaagat cctccacgac cagtggttcc
     7201 tggagtacag cgacccgtgc tggaaggaac aggtcaagac ccagctcgag gagatggcgc
     7261 tcgtcccccc cgaggtcagg gcggagttca tccggacggt cgactggctg aaggactggg
     7321 cctgcacccg gcgggtggga ctcggcacga agcttccctg ggacccgacc tggatcatcg
     7381 aaccgctctc ggactcgacg atctacatgg cctactacac gatcgcccac cacttaaaag
     7441 ccataccgcc ggagaagctg acgcccgcgg tatttgacta tatcttcttt gggatgggtt
     7501 ctccggagac cgtcgaccgg gagaccctcg atgcgatcag gagcgagttc ctctactggt
     7561 atccctacga ctaccggttc tcggcaaagg acctcatatc caaccacctc acgttccagc
     7621 tcttccacca ccgggccata ttcccctcgt acctgcaacc gaaaggcatg gtggtcttcg
     7681 ggatgggcct cctgaacggg gcgaagatgt cctcaagcaa ggggaacgtc ttcctgctcg
     7741 aggatgccct cgaggagttc ggcgccgata ccgtcaggat gttcctcgtc gggagcgccg
     7801 agccctggca ggacttcgac tggaggaacg agctcgtctc gtcgacgaag agacagatcg
     7861 agcggttctg gaacacggtc tccgaggcga tgcaggcgga gggggccacg cccatcgacg
     7921 cctggcttgc aagccggctc gagcgccgta tcgaggccgc caccgcggct cttgactcgt
     7981 tccagacccg gcaggcgctc caggaggcgt tcttcggcgt ggagtccgac ctgaagtggt
     8041 accgccgccg cctgcccgag ggtgcgggcg gcggggcggt gctgcaggac ctctgccgca
     8101 cgtgggtccg cctccttgcc cccttcatcc ccttcacctg cgaagcgctc tggagcgatc
     8161 tcggcgagag cggcatggtc tcctttgccc cctggccgga gacggacgag agccggatca
     8221 accccggagt ggagctcgcc gaggagctcc tcgcgcggac ggtcgaggat atcgagtcga
     8281 tcgtcaaact gataccgatg gagcccgcgt ccatcagtct cttcgtcgcc ccggcctgga
     8341 agtacgaggc gttccggatc atagccggtt cggcggacaa gacgagggtg atgcgggaga
     8401 tcatgcagaa cgagagtatg cggaggcgcg gccgggaggc gacggacgcg gcaaagcaga
     8461 tcacgaaact cgtcctgaag ctgccgcccg atatcgtgaa acagcttgcg gcgtcgcccc
     8521 tcgatgagcg ggcggtcctc gagggcgcac gggcattcct ggagcacgag ttcggcgtgc
     8581 cggtgacggt caaagatgcc ggggagagca accacccgaa ggcggcggcg gccatgccct
     8641 tcaagccggc gatcatgatc gagtagaaac ctcctctttt ttacggcata accggaggga
     8701 tctcatccgc acggttgtcc cgcgatatcc cgcgcatgct caccccgggc ggggtcaggt
     8761 atatctcccc atcacgagac ctgagtatgc cgatgctcct cttcgacgga gagttcgtcg
     8821 gaacccggga acaggacaga cggttatccg gcctgtttgc ggcagcggag taccggcagg
     8881 agcgtatgag ggtgatcgag tcgcggtgcg gtatactctg cagcgggtgc ggctaccggg
     8941 cagagatggg ttgtggggga tgtatggcga taaaaagacc gttctggggc gaaatctgcc
     9001 cgctgaaggc ctgctgcgaa gcgagagggc tggagaactg cggccggtgc ggcgagttcc
     9061 cctgcgaggt tctcgtgcag ttcgcgttcg atgagcggca gggcgacggc ggcaggagaa
     9121 tcgagcagtg cagggcgtgg tctctgcagt agaggcgata atgatggcgt cgagttcgga
     9181 gttcgtggag tacgtggccg ggcaactcgc cggtgcaggc gatatcacgt accggaagat
     9241 gttcggggag tacggtatct actgcgacgg gaagttcttt gcctgtgcct gcgacaacca
     9301 gtttctcgtc aagataacgg aggcgggaaa gaagttcatg ccggacggca ggaccgcatc
     9361 gccgtatccg ggggcaaaac cctgcttcct gatcgacgac ctggatgacc gggagttcct
     9421 gagagaactg acagcgatca cctgtgccgc acttcccgca ccgagaccga agaagaggcg
     9481 ggcggggacg gcggcccggc ggcagggcga agattagata aaaatccggt cccggagcca
     9541 gaaagaggcg gccggccgga gaacccggtt attcgcttcc tttcaaggct tgctgcgctt
     9601 tcttcgcccg ctcttttgcg gtatacccgg tcacctgctg gaggtagttc ctgaaccggc
     9661 ggttggtatc gatgatcgcc tcgtccccgg catccctgtt tgcctcggta tcgacgacca
     9721 gtgttttgcc tcccgcaccg gaccagacct caaggcgatg gagcgccccg taggcgacga
     9781 tatagtggcc gtcggccgcg acttcgggtt cgagcccgaa ctggtcgcgg aggccctgca
     9841 ccatggattt cgcaagatct ttcgtgtatc cacgtttgat ctggtattcc tgcataatat
     9901 ttttaaggga ctgccagcta tgttaaacta atccgggagt gggtgattga tcccatggac
     9961 gatataaagg tcatatccag ggcgcttgcc ggcgccgaaa agacggtgct gatcgggttc
    10021 cccggcagcg ggctcgtcgg gagtattgcc ctgcaatacc tcgtcgagca gatggatttc
    10081 gagcagatcg gggcgatcac gagcaaatac tttccgccgg tcgccctcat gacgcggggc
    10141 gtcatcaacg cacccgtacg cctctacgag aaggatagcc tcgccgcgat catatccgac
    10201 gtccccgtcc atcccatgat ctgctatgag gtggcaaacg gcatcatgga ctggctcacc
    10261 cagttcggca tccgcgagat cgccacgatc gcggggatca tcacaaacga gcccgacaag
    10321 agagtttttg gggttgcaac caccagcacg gtcctccagc ggatcgagga gcagacgatc
    10381 atcctcccca tggggagcat atccggcatc gcggcaagca tcctcacgga gtgcaaaacc
    10441 cggggcatac cggggatcgg actcctcggg gagacggtga acacccccga cccgcggtcc
    10501 tccgcggcca cgatcgaggt cttgaacaag atatacgacc tcgacctgga cgttcagccg
    10561 ctcctcgagc aggcggtgga gatcgaggcg gccatggccc agatcgccga gcaggtgcag
    10621 aagaccgagg cgcaaccacg aagagagcag ctgccgatgt acgggtgatg agtgatgaag
    10681 tgcgcagcat taaccgggat atcaccgtcg gtcattgccg agctcaagac cggcagggca
    10741 cgcaccctcg agctgaacag cgcccacaac atcatcacgc tgaccgaggc cgccccgggc
    10801 gagtgtatct tcatgacgtc ggtcaacggg gaagacctct cgcccgggga ctcggggatc
    10861 atggtcgatc tcctcgccct caccatcagc atgaagcggg tcgaggtcgt caacccgctc
    10921 tactacgagg agcgggaacg gatgtcggcc cgggtcaagg tccagttccg ggggacgacc
    10981 atcgcccgcg gcgtggagga gcgcaagtgg ggtgacccga ccatggtcga gatcatccgg
    11041 tcggcgtgtt accgggccgg gtgagtacgc cctcccttt
//
LOCUS       DAOSEE010000073        11078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_2261_length_11078_cov_256.658079, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000073 DAOSEE010000000
VERSION     DAOSEE010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 11078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11078
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2261_length_11078_cov_256.658079"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PLI76_06800"
     CDS             complement(<1..247)
                     /locus_tag="PLI76_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HOI61776.1"
                     /translation="MMEEILAILLAVAIAATVYYFMKKTLTLVINAVAGLATLLLLNY
                     FDVLAWFGAPDIQVNLVTILICALGGLPGALILVLLHL"
     gene            complement(248..1540)
                     /locus_tag="PLI76_06805"
     CDS             complement(248..1540)
                     /locus_tag="PLI76_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOI61777.1"
                     /translation="MIGDDLIARILREGERRADEVEVFYARGTSVSAEIKKGILGTAE
                     ESEAWSMAIRTVRDGRIGSSSTGDPDRWKECLEAALASGRLATPQQWGGFPKPAEMAG
                     TASSSDPDIVVAIEDAAGMVDALLAGAAEHPVEVVGGSADLARSYIAVANTNGVTYGM
                     DRTVAAVSLEAIREQSTGYEFDASPFRADIDARSVGEEAAALAARSLGGSEVATGRYD
                     VVLSPVAAASILGQVILPALSGRNVKAGRSFLADKVGEQVFDETLSVYDDPFAPGLGS
                     TAWDAEGTPTRRLVFVEQGVLRRFAYDLKTGYRYGEGSTGSAVRSGGDTPGIGFHNLF
                     IDGPRVKEPGDERAVWIHDVVGAHTANPFSGDFSVEISNPFWIEGGDLVEPIRTAMFA
                     GNAFEMLRAIGGLGNDARHVGRLTIPSIRLNNQQIIGK"
     gene            complement(1537..2865)
                     /locus_tag="PLI76_06810"
     CDS             complement(1537..2865)
                     /locus_tag="PLI76_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOI61778.1"
                     /translation="MADVRYYDVRSVRGEVTQIDIDNGVVESAGASFFDKTVIRVLGP
                     RGWGILTRDHVDIDDRRELDGLVAAAARLAAVTKDAISLADAPRGVLAVPPLGEDPRD
                     VDLEEKTRLLAGIEAAARIEGVANTRARYTEGIDAVRFRDSSENEYSFEAVRSGFSVL
                     AIASKNGVMQMGSERDHITTGFNLRDKQGLGRKAGEVAVSLLDAKLPKGGAQRAILDP
                     ELAGVFTHEAVGHASEGDLVREGASVLGGKIGEEIGCRGLVIVDDPSLHEFGFIPVDA
                     EGVAVQRTEIIRDGILRSFLHNRETLAAVGDGIPGHARAEHGAPPIVRMSNTFIENGD
                     ASYDELLAECRDGILLIGSRGGQVDPGRGIFQFNAEYGYIVRGGETTDMVRDVSLSGE
                     ILSTLHNITLIGDDRRMSPGYCGKGGQSVPVSDGAPHLLLEGAVIGGRGE"
     gene            complement(2872..4812)
                     /gene="lonB"
                     /locus_tag="PLI76_06815"
     CDS             complement(2872..4812)
                     /gene="lonB"
                     /locus_tag="PLI76_06815"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease LonB"
                     /protein_id="HOI61779.1"
                     /translation="MDLTTPEEIPKIELTNDELAEADVFAGLELSASSDIDVPPNLID
                     QVIGQEHAVEVIRKAAVQRRHVMMIGTPGTGKSMLAKAMAELLPKEELQDILVYPNSE
                     DNNNPIVRTVPAGRGKQIVNAHKMEARKKIQMRSTLIMLLVFGIIGYAIIASQWLMGI
                     IAAAFVFMALKYSTPREEAMVPKLLVTHDPNTPAPFVDATGSHAGALLGDVRHDPFQS
                     GGLETPSHDRVEGGAIHRAHGGVLFIDEINTLTPHSQQNLLTALQEGTFPITGQSERS
                     SGAMVRTEPVPCRFVMIAAGNLDAIQGMHPALRSRIRGYGYEVYMRDTMEDTPENRKK
                     FLRFIAQEVKNDGKIPHFDRSAMLEVLREARRRSNRKGHLTLKLRDMGGLIRVAGDLA
                     RQEGADITTARHVIAAKSAARSIEDQISDEYIRRSRDYEITVVEGARVGRVNGLAVMG
                     SDSGSVLPVMAEVTPSQGANGSVIATGMLKEIAKESITNVSALIKKFTGKDIRNMDIH
                     IQFIGTYSGVEGDSASVTVATAVISAVENIPVRQDVAMTGSLSVRGDVLPIGGVTYKI
                     EAAAKAGIKRVIIPRSNLEDVLIEDRYRTMVEVVPVEHIEEVLQNALVPENRESFLSK
                     LRKMTVNPTAGIFDPNVGRTLV"
     gene            4894..5751
                     /locus_tag="PLI76_06820"
     CDS             4894..5751
                     /locus_tag="PLI76_06820"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOI61780.1"
                     /translation="MRIISTERSQVLAARVAAKLGVPLVETKFTRFPDGEMYLRCGEL
                     DDETLIVSSTVDNDMFVQTLLLIDAADRSKNTLVAPYLGYSRQDRRFVPGEPISARAV
                     ARALSAGTERAFVVNIHDPGVLQHFGVPAKNITIAPAIGGYIGDLHLKNPLILAPDEG
                     AIEFATGVAAVGGWDCDHLEKTRLSGDEVRIAPKTIDAAGREVVIVDDIISTGGTLAT
                     AACMLREQGAASIHAACVHGVFTSGAYTRLRAAGIASVVSSDTYENASSFISAANAIA
                     TAIRDDAHH"
     gene            5738..6718
                     /gene="rtcA"
                     /locus_tag="PLI76_06825"
     CDS             5738..6718
                     /gene="rtcA"
                     /locus_tag="PLI76_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HOI61781.1"
                     /translation="MLTIDGSCLEGGGQIVRLAVALSALAGIPVAVTRIRENRPNPGL
                     APQQIAAVRAVAGVCNAECRGLTVGSREITFAPGDLRRADLRIDPGTAGSITLILQAW
                     LPVALRTGGSITVTGGTEVAWSPTIDYMDRVLAPVLRNAGASIAIDVLERGYYPRGGG
                     RVHVRVESTCPGPIAIPEGGECGIVSCSAGLPAHVVERQAAAAREILCRDPGLSCTVT
                     TDAREGGAGAGSSVTVWSGAKGAVALGRRGLPAEEVGRTAARALARECRSPGTVDVHL
                     ADQLLIYVACYGGEYTTHTLSMHAKTVCRILPEFGYRVECRENDTVEFSA"
     gene            6715..7170
                     /locus_tag="PLI76_06830"
     CDS             6715..7170
                     /locus_tag="PLI76_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HOI61782.1"
                     /translation="MMFVLDASVFFSDIPIDGPACTTPSVVGELADLHARCRFEVLAA
                     TGLSVREPREEDLARVEAAAVRTGDAGVLSATDRDILALALELPAVLVTDDFAVQNVA
                     HHLGVETRSIRQRPARAIRWRYRCSGCGRYWREPGECRVCGAPIRRKLK"
     gene            7192..7803
                     /locus_tag="PLI76_06835"
     CDS             7192..7803
                     /locus_tag="PLI76_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase-like protein"
                     /protein_id="HOI61783.1"
                     /translation="MSALEELITKAKALQAEGHTPGQISDELGLSMETVTWLLTQPKG
                     MEAPKDVHIDWTAVGSHGTLLNDMAMMMLKRFLYLAEGEGLLTGEIPDTVVGIAPSGV
                     PLATLIAAEEGLKLAVYLPAKHSRSEAPTGSLAGTFATITAQRCIIVDDVVTTGTTLA
                     EVIAFLRRHGATPMAVWSLFDKLGAREVDGVPVLSLFTISRLG"
     gene            complement(7883..9262)
                     /locus_tag="PLI76_06840"
     CDS             complement(7883..9262)
                     /locus_tag="PLI76_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI61784.1"
                     /translation="MRFYGRERELQLMEHLYARAPSFLVVTGRRRIGKTELIKEFCRG
                     KPALYLYVDANKSITALMEEFGRLAAEALNLPGYIRTDTPETFLEFLFSYDQPLIVVF
                     DEFQRFLKIHPSFISQMQRFWDLAGRDSHLFIIISGSSVGMIREIFLLGDAPLFRRAD
                     NILTLRPFSPRECFVILGDLGVQDPVEQLNLYLLFGGTIYYYTFLEKYGCTNLESALD
                     QLILSDLAPLGREMSDVVVEEFGREYATYYEILGAIALGKRTLKEIADLVSLPSTSLP
                     PYMRDLVDLLGIIEYRVPVTERGKRSKMGRYIFVDNFLRFYARFIYRNMSLYESGRFV
                     LLKGRILGEWKGFSGWAFEEMARTLLARNLAEGYEEIGTWWNRRGDEIDLLAFGPEGS
                     LAIEIKNRDLTLGEARAILAALGEKIPLIKGLAGPVTTGIAARTIEGREELATEGFYT
                     ADLGDIRRI"
     gene            9650..>11078
                     /gene="thsA"
                     /locus_tag="PLI76_06845"
     CDS             9650..>11078
                     /gene="thsA"
                     /locus_tag="PLI76_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit alpha"
                     /protein_id="HOI61785.1"
                     /translation="MLAGQPIIILRENVERTRGFEAQRSNIMAAKAIAAAVRTTLGPR
                     GMDKMLVSSSGDVVITNDGATILHEMSVQHPGAKLVVEVAETQDDEVGDGTTTATVLV
                     GSLMEEAERLLAQNVHPTVIANGYQLGMEKALAVLDDLAITIGFEDRENLIKIADTAM
                     TGKSIEAVKEKVSNIVVDAVRQVATGAGAGAYIVDEDDIKIKTQVGDTMHDAELIRGV
                     VIDKKRVFEQMPDAVTDARVALLAQPLEVTKTQVKSKIKITSSDQMKAFTEQERESLK
                     KIADQIAASGANVVLCQKGIADAVQYYLAKSGIYAIEDVKEEDMKFAARALCGTIVNK
                     PEELNATTLGHAEAAEELPDTELTVISGCDNPKAVTILLRGTSQLLLDELERAVYDAA
                     RVVQDAIEDGRFVVGGGSVETELQLRIRDYAATVGGRAQLAIEAFANAFEVIPRTLAE
                     NSGFDTIDKVVALRKAHADGAKYAGL"
BASE COUNT         1999 a         3600 c         3537 g         1942 t
ORIGIN      
        1 ccaggtggag cagcaccagg atcagggcgc cggggagccc cccgagggcg catatgagga
       61 tcgtcaccag gttgacctgg atgtcgggtg ccccgaacca ggcgaggaca tcgaagtaat
      121 tcaggagcaa gagcgtggcg agcccggcga cggcgttgat gacgagcgtc agggtcttct
      181 tcatgaagta atagaccgtc gcggctatcg ctacggcaag aaggatggca aggatctctt
      241 ccatcatcta tttaccaatg atctgctggt tatttaaccg tattgatgga atcgtcagcc
      301 gtccgacatg ccttgcgtca ttcccgagcc cccctatcgc ccggagcatc tcaaaggcgt
      361 ttcctgcaaa catagccgtc cggatgggtt cgacgaggtc tccgccctcg atccagaagg
      421 ggtttgatat ctccacggag aagtcgccgg agaaggggtt tgcagtatgg gcgcccacga
      481 cgtcgtggat ccagaccgcc cgctcgtcgc cgggctcttt cacccgcggc ccgtcgatga
      541 agaggttgtg aaaccctata ccgggagtat cgcccccgga gcgcacggcg ctcccggtgc
      601 tcccctcgcc gtagcggtag ccggtcttga gatcgtaggc aaaccgccgg agcaccccct
      661 gctccacgaa gaccaggcgg cgggtgggag tgccctccgc atcccaggcg gtgctgccga
      721 ggccgggggc gaaggggtcg tcgtagaccg agagggtctc gtcgaagacc tgttcgccga
      781 ccttatccgc aaggaacgac cgaccggcct tcacgttccg cccggagagg gccgggagga
      841 tgacctgccc gaggatgctt gcggcggcaa ccggcgagag gacgacgtcg tagcgcccgg
      901 tcgcgacctc agaacccccg agggagcggg cagcaagcgc ggccgcctct tctccgaccg
      961 accgggcatc gatatcggca cggaacgggg aggcgtcgaa ctcgtagccg gtggattgct
     1021 cccggatcgc ctcgagggag accgccgcca ccgtccggtc catgccgtag gtgacgccgt
     1081 tcgtgttcgc gaccgcgata tacgaccggg cgaggtctgc agaccccccg acgacctcca
     1141 cgggatgctc cgccgcaccc gcgaggagag catcgaccat cccggccgca tcctcgattg
     1201 cgacgacaat atccgggtcc gacgacgatg cggtgccggc catctcggcc ggcttcggga
     1261 acccgcccca ctgctgcggg gtggcgaggc ggccgcttgc aagcgccgcc tccaggcact
     1321 ccttccaccg gtcgggatcg ccggtggatg aggagcctat ccgtccgtcc ctgacggtcc
     1381 gtatggccat gctccaggcc tcggactcct cggcggtgcc gaggatcccc ttcttgatct
     1441 cggcgctgac gctcgtcccc cgggcataga agacctcgac ctcgtcggcc cggcgctcgc
     1501 cttcccggag aatcctcgcg atcaggtcgt cgccgatcat tcgccccgcc ccccgatgac
     1561 cgcgccctca agcaggaggt gcggcgcgcc gtcgctgacc gggacgctct gcccgccctt
     1621 gccgcagtag cccgggctca tccttcgatc gtccccgatg agggttatgt tatggagcgt
     1681 cgagaggatc tcgcccgaga gcgagacgtc gcggaccata tcggtcgtct cgccgcccct
     1741 gacgatatag ccgtactccg cgttgaactg gaagatgccc cgcccggggt cgacctggcc
     1801 gccccttgag ccgatgagga ggatgccgtc ccggcactcg gcgaggagct cgtcatagga
     1861 tgcatcgccg ttctcgatga acgtgttgct catccggacg atcggcggcg caccgtgctc
     1921 cgcccgggcg tggccgggga tgccgtcccc gaccgccgcg agggtctcgc ggttgtggag
     1981 gaacgatcga aggatcccat cgcggatgat ctcggtccgc tgcactgcga caccctcggc
     2041 atccaccggg atgaacccga actcatggag ggagggatca tccacgatca cgagcccgcg
     2101 acagccgatc tcttcgccga tctttccccc gaggaccgat gcgccttcgc ggacgaggtc
     2161 gccctcgctc gcgtggccga ccgcctcgtg ggtgaagacc cccgcaagct ccgggtcgag
     2221 tatggcccgc tgcgccccac ccttcgggag cttcgcatcg aggagcgaga ccgcgacctc
     2281 gccggctttc cggccgaggc cctgcttatc ccggaggttg aacccggtcg tgatgtggtc
     2341 gcgctcgctt cccatctgca tcacgccgtt ttttgaggcg atggcaagga ccgagaatcc
     2401 cgaccggacc gcttcaaagg agtactcgtt ctcgcttgag tcccggaacc ggacggcgtc
     2461 gatcccttcg gtgtaccggg cccgggtgtt tgcaacgccc tcgatccgtg ccgctgcctc
     2521 gatcccggca agcagccggg tcttctcctc gaggtcgacg tcccgagggt cttctccgag
     2581 aggggggacc gcgagaacgc cgcgcggtgc gtcggcaagg ctgatagcat ccttcgtgac
     2641 ggcggcaagg cgtgccgccg ccgcgacgag accgtcgagt tcgcgcctgt cgtcgatatc
     2701 gacgtggtcg cgggtgagga tgccccagcc tctcggcccg agcacccgga tcaccgtttt
     2761 gtcaaagaac gaagcgcctg cggactcgac caccccgtta tcgatatcga tctgggtgac
     2821 ctcgcctcgc acgctcctga catcatagta cctcacgtct gccatacgac atcagaccag
     2881 ggtgcggccg acattcgggt cgaatatgcc ggccgtcggg ttgaccgtca tcttccggag
     2941 cttcgagagg aagctctcgc ggttctcggg gacgagagcg ttctggagaa cctcctcgat
     3001 gtgctcgacc ggcaccacct cgaccatcgt gcggtagcgg tcctcgatga ggacatcctc
     3061 caggttcgac cgcggtatga tcacccgctt gataccggcc tttgccgccg cctcgatctt
     3121 gtaggtcacc ccgccgatgg ggaggacgtc gcccctgacc gagagcgacc cggtcatcgc
     3181 gacgtcctgg cgcaccggta tgttctcgac ggcgctgatc accgccgttg ccacggtcac
     3241 ggaggccgag tcgccctcca cgccgctgta ggtgccgatg aactggatgt ggatatccat
     3301 atttctgata tccttgcccg tgaacttctt gatcagggcg ctgacgttcg tgatcgattc
     3361 tttcgcgatc tcttttaaca taccggtcgc gatcaccgaa ccgttcgccc cctggctcgg
     3421 tgtcacctcg gccatcaccg ggaggaccga tcctgagtcg ctccccatca ccgcaagccc
     3481 gttcacccgg ccgacccggg cgccctcgac gacggtgatc tcgtagtcgc ggctccgccg
     3541 tatgtactcg tcggatatct gatcctcgat cgagcgggcg gccgacttcg cggcgatgac
     3601 gtgccgtgcc gtggtgatat cggcaccttc ctgtcgggcg aggtcccccg ccacccggat
     3661 gagcccgccc atatcgcgga gtttcagggt caggtggccc ttccggttcg agcggcggcg
     3721 ggcctccctg agcacctcga gcatggcgct ccggtcgaag tgggggatct tcccgtcgtt
     3781 cttgacctcc tgggcgatga atcggaggaa cttcttccgg ttctccggcg tatcctccat
     3841 cgtgtcccgc atgtagacct cgtagccgta gccgcggata cgcgaccgga gcgccgggtg
     3901 catcccctgg atggcatcga ggtttccggc cgcgatcatg acaaatcggc agggaaccgg
     3961 ttcggtccgg accatcgcac cgcttgaacg ctcgctctgg ccggtgatcg ggaaggtgcc
     4021 ctcctggaga gcggtcagca ggttctgctg ggagtgcggc gtgagggtgt tgatctcgtc
     4081 gatgaagagc accccgccgt gcgcccggtg aatggccccg ccctcgacac ggtcgtggga
     4141 cggtgtctca aggccgccgc tctggaacgg gtcgtgccgc acgtcgccga gcagggcacc
     4201 cgcgtgcgag ccggtggcgt ccacgaaggg cgcgggggtg ttcgggtcgt gggtgaccag
     4261 gagcttcgga accatcgcct cctcgcgggg cgtcgagtac ttcagcgcca tgaagacgaa
     4321 cgcggcggcg attattccca taagccactg ggaggcaatg atggcgtagc cgatgatacc
     4381 gaagacaagg agcatgataa gagtgctccg catctggatc ttctttctcg cctccatctt
     4441 gtgggcgttg acaatctgct tgccgcgacc ggcgggaacg gtccggacga tcgggttatt
     4501 gttgtcctcg gagttcggat agaccaggat atcctgcagt tcctctttcg ggaggagctc
     4561 cgccatcgct ttggccagca tcgatttgcc ggtcccgggg gtgccgatca tcatcacgtg
     4621 cctgcgctgg accgctgcct tccggatcac ctcgacggca tgttcctgac cgatgacctg
     4681 atcgatgaga ttcgggggta catcgatatc tgacgatgcg ctcagctcaa ggccggcaaa
     4741 gacgtcggct tccgccagct cgtcgtttgt gagctcgatc ttcgggattt cttctggagt
     4801 cgtcaaatcc atcgtcgggt ttacctcttt gatgcactga tacttatata atttattcat
     4861 cctcgttcaa atactttcca gcaaaggtgt tacatgagga taatcagcac ggaacggtcc
     4921 caggtcctcg cggcccgcgt cgccgcgaag ctcggggtac cgctggttga gacgaaattc
     4981 acccgatttc ccgacgggga gatgtatctg cggtgcgggg aactggacga cgagaccctg
     5041 atcgtcagca gcaccgtgga caacgatatg tttgtgcaga cgctccttct gatcgatgcc
     5101 gccgaccgat cgaagaacac cctggtggcc ccgtacctcg gctactcccg ccaggacagg
     5161 cggtttgtcc cgggtgagcc gatcagcgcc agggcggtcg cacgagctct ctctgccggc
     5221 accgagcggg cgttcgtggt caacatccac gaccccggcg tgctgcagca cttcggcgtc
     5281 ccggcaaaga acatcaccat cgctccggcc atcggggggt atatcggcga cctgcacctg
     5341 aagaaccccc tcatccttgc cccggacgag ggagcgatcg agttcgcgac cggcgttgcg
     5401 gcggtcggcg ggtgggactg cgaccacctt gagaagaccc ggctctcggg cgacgaggtc
     5461 cggattgccc caaagaccat cgatgccgcc gggcgcgagg tcgtgatcgt ggatgatatc
     5521 atatccaccg gcggaacgct tgcgaccgcg gcctgcatgc tccgggaaca gggagcggca
     5581 tccatccacg cggcctgcgt ccacggggtg tttacgagcg gtgcctacac gcgcctgcgg
     5641 gcggcgggga tcgcctcggt cgtctcaagc gacacctacg agaacgcctc aagcttcata
     5701 tcggccgcga acgccatcgc caccgcgatc agagacgatg ctcaccattg atgggtcctg
     5761 cctcgagggg ggcgggcaga tcgttcgcct ggcggtcgcc ctctcggcgc ttgccgggat
     5821 accggtcgcc gtgacccgga tacgggagaa ccggccgaac cccggtcttg ctccccagca
     5881 gatcgccgcg gtccgggcgg tcgcgggggt ctgcaacgcc gaatgccggg ggcttacagt
     5941 cgggagccgc gagatcacgt tcgctccggg cgacctccgg cgggccgacc tccggatcga
     6001 tccggggact gccgggagca tcaccctcat cctccaggcg tggctcccgg tcgccctccg
     6061 caccgggggg agcataacgg tcacgggggg gaccgaggtg gcctggagcc cgacgatcga
     6121 ttacatggac cgcgtcctcg caccggtgct ccgcaacgcc ggggcctcga tcgctatcga
     6181 cgtgctcgag cggggttact acccgcgggg aggggggcgg gtccacgtca gggtggaatc
     6241 gacctgcccg ggcccgatcg cgatcccgga ggggggggag tgcggtatcg tctcctgctc
     6301 ggcaggcctc cccgcacacg tcgtggagcg gcaggcggcc gcggcccggg agatcctctg
     6361 ccgggatccg ggcctctcct gcacggtgac cactgacgcc cgggagggcg gggcgggcgc
     6421 ggggagttcc gtgacggtat ggagcggggc aaaaggcgcc gtcgctctcg ggcgacgggg
     6481 gctccccgcc gaggaggtgg gccggacggc cgcccgggcc ctcgcacggg agtgccgctc
     6541 ccccggcacg gtcgacgtcc acctcgccga ccagctcctc atctacgtcg cctgctacgg
     6601 cggggagtac accacgcaca cgctctcgat gcacgcgaag accgtctgcc ggatcctccc
     6661 ggagttcggc taccgggtcg agtgccggga gaacgacacc gtggagttct ccgcatgatg
     6721 ttcgtcctcg acgcctcggt ctttttttcg gatatcccga tcgacggccc ggcctgcacc
     6781 accccgtcgg tcgtcgggga gcttgccgac ctccacgcac ggtgccggtt tgaggtgctc
     6841 gcagcaacgg ggctctccgt ccgggaaccg cgggaagagg acctcgcacg ggtcgaggcg
     6901 gctgcagtcc ggacggggga cgccggggtg ctctccgcga ccgaccgcga tatactcgcg
     6961 ctcgccctgg aactcccggc cgtcctcgtc accgacgact tcgctgtcca gaatgtcgcc
     7021 caccatctcg gcgtcgagac ccggagcatc cgacagcggc ccgcccgggc gatccggtgg
     7081 cgctaccggt gcagcggatg cggccggtac tggcgggagc cgggggagtg ccgggtctgc
     7141 ggcgcaccga tcagaagaaa acttaaatag gtcactaaaa gattcttctc catgtccgcc
     7201 cttgaagagt tgatcacgaa ggcaaaggcg ctccaggcag aagggcacac cccgggccag
     7261 atatccgatg aactcggcct ctcgatggag accgtcacct ggctgctcac ccagccaaag
     7321 gggatggagg ctccaaaaga cgttcacatc gactggacgg cggtagggag ccacgggacg
     7381 ctcttaaacg atatggcgat gatgatgctc aagcgcttcc tctacctggc ggagggggaa
     7441 gggctgctga ccggggagat cccggatacg gtcgtcggta tagcgccctc gggcgtcccg
     7501 ctcgcgaccc tcatcgccgc agaggaaggg ctcaaactcg cggtctacct tcccgcaaag
     7561 cacagccgga gcgaggcgcc caccgggtcc ctcgcgggca cgttcgccac gatcaccgcc
     7621 cagcgctgca tcatcgtcga cgacgtcgtc acgaccggga cgacgcttgc ggaggttata
     7681 gcgttcctcc ggcggcatgg ggcgacgccg atggcggtct ggtcgctctt tgacaaactg
     7741 ggcgcccggg aggtcgacgg cgtcccggtc ctgtcgctct ttacgatatc gaggctcggg
     7801 tgagagtcaa aactacctgt tttttcggga ggcgcgggca ggagaacccg gggataccac
     7861 cccgcctccc gcgcccggcc cctcagatcc tcctgatgtc accgagatcc gccgtgtaga
     7921 acccttccgt cgcgagctcc tccctgccct cgatggtgcg ggcggcgatg ccggtggtca
     7981 ccggcccggc gagacctttt atgaggggga tcttctcccc cagagcggca aggatggcgc
     8041 gtgcctcgcc gagggtgaga tcgcggttct tgatctcgat ggcaaggctc ccctccgggc
     8101 cgaaggcgag aagatcgatc tcatcgccac ggcgattcca ccaggtgcca atctcctcgt
     8161 agccctctgc gaggttccgg gcgaggaggg tccgcgccat ctcctcgaag gcccagccgg
     8221 agaacccctt ccactcgccg aggatccggc ccttcagaag gacaaagcgc ccgctttcgt
     8281 aaaggctcat gttccggtag ataaagcggg cgtagaatcg gagaaaatta tcaacaaaga
     8341 tgtatctgcc catcttcgag cgtttccccc tctcggtgac cggaacccgg tactcgatga
     8401 tcccgaggag gtcaacgaga tcccgcatgt acggggggag cgatgtcgag gggagggaga
     8461 cgagatcggc gatctccttc agggttcttt tcccaagagc aatggccccg aggatctcgt
     8521 aataggtcgc gtattcccgc ccgaactcct cgaccacgac gtcactcatc tcccggccga
     8581 ggggagcaag gtcactcagg atgagctgat cgagggcact ctcaaggttg gtacatccgt
     8641 acttctcaag aaaggtatag tagtagatcg tcccgccgaa gaggagatag aggttgagct
     8701 gctccaccgg atcctgcaca cctagatcgc cgaggatgac aaagcattct cttgggctga
     8761 aggggcgcag tgtcaggatg ttgtcagccc gcctgaagag aggggcatcc cccagaagga
     8821 agatctcccg gatcattccc accgacgacc cggatatgat gatgaagaga tgcgaatccc
     8881 ggcccgcgag atcccagaac cgttgcatct gagatataaa cgaggggtgg atcttaagga
     8941 aacgctggaa ctcatcgaag acgacgatca gcggttggtc gtaggagaag aggaactcaa
     9001 ggaacgtctc cggcgtatcg gtcctgatgt aaccagggag gttgagcgct tccgctgcca
     9061 acctcccgaa ctcctccatg agagccgtga tgctcttgtt cgcatcgacg tagaggtaga
     9121 gggccggctt ccctctacag aactctttga tcagctcggt cttgccgatc cttcgcctgc
     9181 cggtgacgac cagaaaggag ggggcacggg catagaggtg ctccatcagc tggagttccc
     9241 gctccctgcc atagaacctc atagttatta tggatataat aattacaaat ataataatta
     9301 cggtgccacc caccccgccg ccggcggaga ggtactcttt gggagcggca ttttcccatc
     9361 accgatagag agactcttgc aacccctttt cggaaggacc gggtctccct gcagagataa
     9421 accggagtgt ttgccccggg caaccccgac cgccctcgcc ctgcccggat cttccggata
     9481 cgggggaaga ctccgcacct cacgcatccc cccggacccc gggcgtgcag aaccgatctc
     9541 ccggcgatcc ggcgcctccc gacggcccgg gcaccccggg gtgaccgggc ctatcgatca
     9601 ctatatatag aacgtctgaa caaacctaac ggacaaagga gattatacga tgcttgctgg
     9661 acagcccatc attattttac gggaaaacgt tgagcgcacg cgcggattcg aggcgcagcg
     9721 ctcgaatatc atggctgcaa aggcaattgc ggccgccgtt cggacaacgc tcggtccccg
     9781 gggaatggac aagatgctgg tcagctccag cggtgacgtg gtgatcacga acgacggggc
     9841 aaccatcctg cacgagatgt ccgtgcagca ccccggcgcc aagctggtcg tcgaggtcgc
     9901 cgagacccag gacgacgagg tcggcgacgg caccacgacc gcgacggttc tggtcgggtc
     9961 gctgatggaa gaggcggaga gactgctcgc ccagaacgtt caccccacgg tcatcgcgaa
    10021 cggttaccag ctcggcatgg agaaggccct cgcggttctt gatgacctgg ccataacgat
    10081 cgggtttgaa gaccgtgaga acctcataaa gatcgccgac accgctatga ccggcaagtc
    10141 catcgaggcg gtcaaggaga aggtatccaa catcgtcgtg gatgcggtgc ggcaggtcgc
    10201 gaccggggcg ggcgccggcg cctacattgt ggacgaggac gacatcaaga tcaagacgca
    10261 ggtcggcgac acgatgcacg acgcggagct catccggggc gtcgtcatcg acaagaagcg
    10321 cgtctttgaa cagatgccgg acgcggtcac cgacgccagg gtagccctgc tcgcccagcc
    10381 gctcgaggtc acgaagaccc aggtaaaatc aaagatcaag atcacctctt cagaccagat
    10441 gaaggcgttc accgagcagg aacgcgagtc cctcaagaag atcgccgatc agatcgccgc
    10501 ctcgggtgca aacgtcgtcc tctgccagaa ggggatcgcc gatgccgtcc agtactatct
    10561 cgcgaagagc ggcatatatg ccatcgagga cgtaaaggaa gaggacatga agttcgcagc
    10621 acgggcactc tgcggcacca tcgtcaacaa gcccgaggaa ctcaacgcaa cgacgctcgg
    10681 ccacgccgaa gcggccgaag agctcccgga caccgaactg acggtcatat ccgggtgcga
    10741 caacccgaaa gccgtcacga tcctcctccg gggcacctcc cagctcctgc tcgacgagct
    10801 cgagcgggcg gtctacgacg cagcccgggt cgtccaggac gccatcgagg acggcaggtt
    10861 cgtcgtcggc ggcgggtcgg tggagaccga gcttcagctc cgcatccgcg actacgcggc
    10921 aaccgtcggg ggccgggccc agcttgccat cgaagccttt gcgaacgcgt tcgaggtcat
    10981 cccgaggacg ctcgcggaga actccgggtt tgacacgatc gacaaggtcg tcgctctccg
    11041 gaaggcccac gccgacggcg cgaagtacgc cgggctca
//
LOCUS       DAOSEE010000074        10898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_2314_length_10898_cov_287.618371, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000074 DAOSEE010000000
VERSION     DAOSEE010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 10898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10898
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2314_length_10898_cov_287.618371"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="PLI76_06850"
     CDS             complement(<1..663)
                     /locus_tag="PLI76_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HOI61786.1"
                     /translation="MSLATIREKLRFGSFFRRRTPAIRGPASRDREAEMRALLARPPF
                     AITDIADAALPLYDRALTHRSYANGSEYPIAAADDNERLEFLGNYVLDFVIADHLYGE
                     YDLPPGEMNRRLQVTRNTKLADIVHRQGLGIDGLIRRRGQAPTDSIIADAFEALIGAI
                     YVDRGIGAAREVVLAIFEDEIAGCDTRRNYRGRLQELVARKGLGGIDYIYRRTGPGSS
                     PVW"
     gene            774..1520
                     /locus_tag="PLI76_06855"
     CDS             774..1520
                     /locus_tag="PLI76_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61787.1"
                     /translation="MQSAAVGGGGSVYLDIEFGYVYGTSRAVMMPVEIGAVIHHPEDD
                     SVRYAGEQFRYDIDVEVWKKVTDPCGRTVGVATTVANMGRGRYGGAYDHYFRLPGDRV
                     PAAEETAGKAFADLRVFMESLLTDDITEIVVFAADMERRAFRTADVPLDGRRLVDLQR
                     EIRRRLGMKQVLSLDRLARLIDFSAENGAVASTHFRYPVPPGYRHLLDVHRGMGDAVR
                     MFLLAREFREKLPELEKRVRALEDTCGGEE"
     gene            complement(1483..2733)
                     /locus_tag="PLI76_06860"
     CDS             complement(1483..2733)
                     /locus_tag="PLI76_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HOI61788.1"
                     /translation="MQTSTPGSNFFHRKRERLALKPLGDLRAMMRSTFPCPDRTTRVP
                     VAASAGRVTARPVHSPMTVPATDTAARDGFAVISAETRGAGDATPVRLRNPHRVNTGN
                     AIPPGYDAVVMIEDIAGEDGTWSTRKAAAPGEHIHPAGSEIEQGERILPAGHRIRPCD
                     IGALLTYGIAEIDVPAVKVGLIPTGNELVPAGGHPGAGEVIESNTAVAAAWLCEAGAS
                     PTRYGIVADDPAALRQAIAKAVRENDLVLVSAGSSTGTRDFTAGVIGELGEVLVHGIA
                     MKPGRPAIVGRVEDKPVIGMPGYPIAALTTIRELALPLLAEWGFCAPPAERLRARLCE
                     SVLADPEFDEFILLTVYRADDGYTAVPLPPHSRTQMAMVRANAYLHVPAGSGAIPKGT
                     EVDVILTGPCAPVTPRPRRYPQEP"
     gene            complement(2708..3508)
                     /locus_tag="PLI76_06865"
     CDS             complement(2708..3508)
                     /locus_tag="PLI76_06865"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018493 - formylmethanofuran dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit C"
                     /protein_id="HOI61789.1"
                     /translation="MKTVTITLVNPPELYLEGQNITPDNVAGKTAADIATLDVWEGKQ
                     KSPLGKYFTVSGNGGATAADTKIVFRGDCTRVKRIGQQMTAGEIVVEGNADMYIGGWM
                     KGGKIHVKGNVDSFCGIGMEGGELIVDGNAGHHLGSSPRGEWRGMQGGLIRVAGDAGN
                     DTATFINGGTIIIGGNADIHVSTHGEGGTVIIKGNAKGRVGGQMVKGDIYVFGTIENP
                     LPGFQRVGEVEQEVAGEKACFIHHVGDLGERHPIAKGKAVYANLYTRI"
     gene            complement(3522..5228)
                     /locus_tag="PLI76_06870"
     CDS             complement(3522..5228)
                     /locus_tag="PLI76_06870"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit A"
                     /protein_id="HOI61790.1"
                     /translation="MGELIVKNGFVFDPLQGIKGDRMDVGVRDGKIVETNTLKNPKEI
                     DAAGMTVMAGGVDIHAHVVGPKVNVGRLFRPEDKLFKSPLKRGNRMEMGFSVPSTFKT
                     GYDYARMGYVFVNEAAMPPLHSPHVHEEIRDTPIIDNAAMPVFGNNWFTLDYLKNNEI
                     ENNAAYTAWLLRATRGFAIKVVNPGGTEAWGWGLNCENIHDTVPYFDITPAKIITGLI
                     ETNEFLGLPHSMHLHTNNLGNPGNYTDTLDSLRLAEGYTAKNNFGRDQVMHVTHVQFC
                     SYGGDSWKNIDSQAKTIMDYVNAHDNITIDMGQVTLDETTTMTADGPFEHHLRGLNNL
                     KWANTDVELETAAGVVPYIYSPNVKVSTIQWAVGLELALYSNDLMRTFITTDHPNAGP
                     FTRYPRVIKWLMSRAARDATMKTFKWLDRTIESTTIDSIDRELTLYEIAQMTRAGPAK
                     ALGISNTYGGLAPGMDGDIAVYALNPENMPSDPDRIEEAFSRCAYLVKDGVVTARGGE
                     VVTEPAKRTIWVDVKVPENAQVQRDIYEHFLRHYSVGVKNYQLFDEHLHNPRVIEVDT
                     TQ"
     gene            complement(5232..6599)
                     /locus_tag="PLI76_06875"
     CDS             complement(5232..6599)
                     /locus_tag="PLI76_06875"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit B"
                     /protein_id="HOI61791.1"
                     /translation="MAKKIENVGCPFCGVCCDDLVVTVSDDGKKILEVENACIIGSTV
                     FHRAMEDRHALPRLRQPDGTFKEISYDEAIDYTAKVLHNAKKPLIYGFGSTNCEGMAA
                     AAKVAEKAGAVLDNCASICHGSSFLAIFDNGYPSCTLGETKNRADVVLYWGANPAHAH
                     PRHMSRYSIFPRGFFTGKGQKSRKIIVIDPRHTDTAQVADIYLQVRQGHDYELFDAFR
                     AVVRGHEIPEEVAGIKREQILEVANIMKKARFGTIFYGMGLCHSDGRNHNVDIAISLT
                     RDLNDYTKWTIMAMRGHYNITGPGAVWSWQYGFPYCLDLTKRDIAHMNPGETSSVDLA
                     MRDEVDAFINIGTDAAAHFPIEPVKHLRKHPWIAIDPHPNMATEISDLHIPVGIVGVE
                     VPGIVYRMDNVPIQYRKVIDPPEGVISDEELFERIHLRLCELEAEDAVNVPAKAAPEG
                     VRAGE"
     gene            complement(6599..7015)
                     /locus_tag="PLI76_06880"
     CDS             complement(6599..7015)
                     /locus_tag="PLI76_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HOI61792.1"
                     /translation="MAKKITLNMITQRSVEEGVGIVKGKMTPDYFEACSIIEMHDDDV
                     RALGLVPNQLVRVTSECGSVVVKSVRGRQALYPGLAFIPQGVYANQIVPARTQATGEP
                     QYSGFPVTVEPVAPGERRKSTLELVQSAVGMWKGDQ"
     gene            8337..9641
                     /gene="mcrB"
                     /locus_tag="PLI76_06885"
     CDS             8337..9641
                     /gene="mcrB"
                     /locus_tag="PLI76_06885"
                     /EC_number="2.8.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme-B sulfoethylthiotransferase subunit
                     beta"
                     /protein_id="HOI61793.1"
                     /translation="MAKYTETIDLYSDDGKLLKGGVTLDRISPLVNPATSKIIDLTKR
                     TINVNLGGIQDALKTGKLGKGKIKGRELDLPIMENKDAIVARIKDMIQVTEGDDTEIM
                     EFNGGKLQLVKVPAKRLANAATYDAAITSVAAATTYAIVEEFGIDAFNASTVKAACWG
                     GYPHTQDLEGALVTSILNIPQNNEGLGYALRNIPVNHTVMMTGRNALQGAALASTLET
                     AGVFEMGGAVGPFERAMLLAYAYQGLNANNMVYDLVKANGATGTIGTVVQSLVERAIE
                     DKVITPGKKGGYFQFYDTKDPMLWNAYAAAGTMAATIVNCGAGRFAQAVSATLLYFND
                     LLEHETGLPSTDYGRVMGTAVGFSFFSHSIYGGGGPGIFNGNHVVTRHANGVAIPCVV
                     AAAALDAGTQMFSPESTSKIFADTYGKIDVFNKPIDQIANGA"
     gene            9852..10616
                     /gene="mcrG"
                     /locus_tag="PLI76_06890"
     CDS             9852..10616
                     /gene="mcrG"
                     /locus_tag="PLI76_06890"
                     /EC_number="2.8.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme-B sulfoethylthiotransferase subunit
                     gamma"
                     /protein_id="HOI61794.1"
                     /translation="MAYKPQYGPGTSIVAANRRKQMNPGQKLEKVRSVTDEDIVLILG
                     HRAPGSAYPSAHPPLGEQQEPDCPMRRIVKPTEGASAGDRVRYIQFADSMFNAPSQPY
                     QRTYTEMYRFRGIDPGTLSGRQIVECRERDLEKYAKELIETELLDPARTGIRGATVHG
                     HSLRLAEDGMMFDMLQRNILGEDGVVRYVKNQIGEPLDRAVAVGKPLDEKWLKAHTTI
                     FHSLVGTPYRDDTEYIEYVQRIHSLRTKYGFMPREE"
     gene            10622..>10898
                     /locus_tag="PLI76_06895"
     CDS             10622..>10898
                     /locus_tag="PLI76_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-coenzyme M reductase subunit alpha"
                     /protein_id="HOI61795.1"
                     /translation="MAKIERSQKLFLKSLKEKFQGQDVESETAEFYKFNGVRQSPRKV
                     EFMKASLAAEMDRGISMYDPERCHLGGIPMGQRQLMTYEVSGTGVFVE"
BASE COUNT         1897 a         3343 c         3446 g         2212 t
ORIGIN      
        1 ccagacggga gaactccccg gacccgtccg gcggtagatg tagtcgatcc cgccgaggcc
       61 ctttcgcgcg acaagctcct ggagcctccc ccggtagttc cggcgggtgt cgcacccggc
      121 tatctcgtcc tcgaatatgg cgaggaccac ctcccgggcc gcgccgatgc cccggtcgac
      181 gtatatggcg ccgatgagag cctcgaacgc atcggcgatg atcgagtcgg tcggcgcctg
      241 cccgcgcctc ctgatgagcc cgtcgatgcc gaggccctgc cggtgcacga tatcggcgag
      301 tttggtgttc ctcgtcacct ggagccgccg gttcatctcg ccgggcggaa ggtcgtactc
      361 cccgtagagg tggtcggcta tgacgaagtc gaggacgtag ttccccagga actcgagccg
      421 ctcgttgtcg tcggccgccg ctatcgggta ctcggagccg ttcgcgtatg accggtgggt
      481 cagcgcccgg tcgtagaggg ggagggcggc gtcggcgata tcggtgatag caaacggggg
      541 gcgggcgagg agcgcgcgca tctccgcctc ccggtcgcga gaggcgggcc cccggattgc
      601 gggggttctc ctccggaaga aggagccaaa acgaagtttt tcccggatgg tggcgaggct
      661 catggtcgtc tggtgcaggg tatcctgttg ttcgtataga gaggctctcg tgctctccga
      721 taaacctttg ccgggaaacc ttttcgtcgt ctccgaatat acttctctct ggtatgcagt
      781 ccgcggcggt cgggggcggg gggagcgtgt atctcgatat cgagttcggg tatgtctacg
      841 gcaccagccg ggcggtcatg atgccggtcg agatcggcgc cgtcatccac cacccggaag
      901 acgacagcgt ccgctacgcc ggggagcagt ttcgctacga catcgacgtc gaggtatgga
      961 agaaggtgac cgacccctgc ggcaggaccg tcggcgtcgc gacgaccgtg gcgaatatgg
     1021 gccgcggcag gtacggtggg gcctacgacc actacttccg gctcccgggc gatcgggtcc
     1081 ctgcggcgga agagactgcc gggaaagcgt ttgccgacct ccgggtcttc atggagtctc
     1141 tcctgacaga cgatatcacg gagatcgtcg tctttgcggc cgacatggag cggagggcct
     1201 tccggaccgc ggatgtcccg cttgatgggc gccggctggt cgatctccag agggagatac
     1261 ggcgccgcct cggcatgaag caggtcctct ccctcgaccg ccttgcccgg cttatcgatt
     1321 tttccgccga gaacggagcc gtcgcctcga cgcattttcg gtacccggtc ccccccgggt
     1381 accgccacct cctcgacgtc caccggggga tgggcgacgc cgtccggatg ttcctgctcg
     1441 cgcgggagtt ccgggagaag ctccccgaac tggagaagcg ggtcagggct cttgaggata
     1501 cctgcggggg cgaggagtga ccggtgcgca cggcccggtc aggatgacgt cgacctctgt
     1561 tcctttcggt atagctccgc tccccgccgg gacgtgcagg taggcgtttg cgcggaccat
     1621 cgccatctgc gtccttgagt gcgggggcag gggaacggcg gtatacccgt cgtcggcccg
     1681 gtatacggtg aggaggatga actcgtcgaa ctccggatcg gccaggaccg attcgcaaag
     1741 ccgggcacga aggcgttctg ccggcggggc gcaaaaaccc cattccgcga gaagggggag
     1801 ggcgagctcg cgtatggtcg tcagcgcggc gatcgggtag cccggcatac cgatgaccgg
     1861 tttgtcctcg acccgcccca cgatcgccgg tctcccgggc ttcatcgcga tgccgtggac
     1921 gagcacctcg ccgagttcgc ctatgacgcc ggcggtgaag tcccgtgtgc ctgtcgagga
     1981 gccggcggag accaggacca gatcgttctc ccggacggcc tttgcgatcg cctgccggag
     2041 cgcggcgggg tcgtccgcga cgatgccgta gcgggtcgga gaggcgccgg cctcgcaaag
     2101 ccacgccgct gccacggcgg tgttgctctc gatcacctcg cccgcgcccg ggtgcccgcc
     2161 cgccgggacc agctcgtttc ccgtgggtat gaggccgacc ttcacggccg gtacatcaat
     2221 ctccgcgatg ccgtaggtga ggagcgcacc gatatcgcag gggcgtatgc ggtggccggc
     2281 cgggaggatc ctctctcctt gctcgatctc ggatcccgcg gggtggatat gttcccccgg
     2341 tgccgcggcc tttcgggtgc tccaggtacc gtcctctccg gcgatatcct cgatcatgac
     2401 gacggcgtcg taccccgggg ggatcgcgtt tcccgtgttc acccggtgag ggttccggag
     2461 ccggacgggg gtcgcatcac ccgccccgcg ggtctcggcg cttatgacgg caaaaccgtc
     2521 ccgtgcggcg gtatccgttg ccgggaccgt catgggcgag tggacgggcc gtgccgtcac
     2581 ccgtccggcc gatgcggcga cgggaaccct cgtcgtccgg tccgggcacg ggaatgtgga
     2641 gcgcatcatg gcccggagat cgccgagcgg tttcagggcg aggcgctctc ttttccggtg
     2701 aaaaaaatta gatccgggtg tagaggtttg catacaccgc cttgcccttc gcgatcgggt
     2761 ggcgctcgcc aaggtccccg acgtggtgga tgaagcaggc cttctcgccg gcgacctcct
     2821 gttccacctc gccgaccctc tggaatcccg ggagcgggtt ctcgatcgtt ccgaagacgt
     2881 atatgtcgcc tttcaccatc tggccgccga cgcggccttt ggcgttgccc ttgatgatga
     2941 cggtgccgcc ttcgccgtgg gtggagacgt ggatgtccgc gttgccgccg atgatgatcg
     3001 tcccgccgtt gatgaacgtg gccgtgtcgt tgccggcatc cccggcgacc cggatcagcc
     3061 cgccctgcat gccgcgccac tcgccgcggg gcgacgagcc caggtggtgc ccggcgttcc
     3121 cgtcgacgat cagttcgccg ccttccatcc ctatgccgca gaacgagtcg acgtttcctt
     3181 tgacgtgaat cttcccgccc ttcatccagc cgccgatgta catgtcggcg ttgccttcga
     3241 cgacgatctc gcctgcggtc atctgctggc cgatcctctt cacacgggtg cagtccccgc
     3301 gaaagacgat cttcgtatcg gccgcggttg ctccgccgtt gcccgagacc gtgaagtact
     3361 tcccgagcgg gctcttctgt tttccctccc agacgtcgag ggtggctata tcggctgccg
     3421 tcttgccggc aacgttgtca ggcgtgatgt tctgcccctc aagatagagt tcggggggat
     3481 ttaccagggt gatagttact gtcttcatgc ttcccgtcac ctcactgtgt cgtatcgacc
     3541 tcgatcaccc gcgggttgtg gaggtgctca tcgaagagct ggtagttctt gactcccacg
     3601 ctgtagtgcc tgaggaagtg ctcgtagatg tctctctgca cctgtgcgtt ctccggaacc
     3661 ttcacgtcga cccagatggt ccgctttgcc ggctccgtga cgacttcgcc gcctctggcg
     3721 gtcacgacgc cgtctttgac gaggtaggcg caccgcgaga atgcctcctc gatcctatcg
     3781 gggtcggagg gcatattctc ggggttgagg gcgtagacgg ctatgtcgcc atccatgccg
     3841 ggcgcgagcc cgccgtaggt gttgctgatg ccgagcgcct ttgcgggtcc cgcccgggtc
     3901 atctgcgcga tctcgtagag cgtgagctcg cggtcgatgc tgtcgatggt ggtggactct
     3961 atcgtccggt cgagccactt gaacgtcttc atggtcgcgt cacgtgcggc cctgctcatc
     4021 agccacttga tgaccctcgg gtagcgggtg aacggcccgg cgttcgggtg gtcggtggtg
     4081 atgaacgtcc gcatcaggtc gttggagtag agcgcgagtt ccagaccgac ggcccactgg
     4141 atggtggaga ccttgacgtt cgggctgtag atgtagggga cgacacccgc cgcggtctcg
     4201 agctcgacgt cggtgtttgc ccatttcagg ttgttgaggc cccggaggtg gtgctcgaac
     4261 ggcccgtcgg cggtcatcgt cgtggtctcg tcgagtgtca cctggcccat gtcgatggtg
     4321 atgttgtcgt gcgcgttcac gtaatccatg atcgttttgg cctgagagtc gatgttcttc
     4381 caggaatcgc cgccataaga gcagaactgg acgtgcgtga cgtgcatgac ctggtcgcgg
     4441 ccgaagttgt tcttcgccgt gtagccctct gcaagcctga gggagtcgag ggtgtcggtg
     4501 tagttgcccg ggtttccgag gttgttcgta tgcaggtgca tcgagtgggg gaggcccagg
     4561 aactcgttcg tctcgatgag acccgttatg atctttgccg gggtgatatc gaagtagggg
     4621 accgtatcgt gtatattctc gcagttcaga ccccatcccc aggcttccgt gccgccgggg
     4681 ttgacgacct tgatggcaaa cccgcgggtt gcccggagaa gccaggcggt gtaggcggca
     4741 ttgttctcga tctcgttgtt cttgaggtaa tcgagcgtga accagttgtt cccgaagacc
     4801 ggcatcgccg cgttgtcgat gatcggcgtg tcgcggatct cctcgtggac gtggggggag
     4861 tggagcgggg gcatcgccgc ttcgttgacg aagacgtagc ccattcgggc gtagtcgtag
     4921 ccggtcttga acgtggaggg gacggagaac cccatctcca tgcggttgcc ccgcttgagg
     4981 gggctcttga agagtttgtc ttccggccgg aagagccggc cgacgttcac cttcggcccg
     5041 acgacgtggg cgtggatatc cacgccgccc gccatgacgg tcatgccggc ggcgtcgatc
     5101 tctttcggat ttttaagggt gttcgtctcg acgatcttgc cgtctctgac gccgacgtcc
     5161 atccggtcgc ccttgatgcc ctgcaggggg tcgaatacga acccgttctt gacgatgagt
     5221 tcacccatgg ttcactcccc cgcgcggaca ccctcgggcg cggcctttgc cggcacattt
     5281 acggcatctt ccgcttccag ctcgcagagc ctcaggtgta tccgctcgaa gagttcttcg
     5341 tcgctgatga cgccctcggg cgggtcgatg accttgcgat actggatggg gacgttgtcc
     5401 atccgataga cgatgcccgg aacctcgacg ccgacgattc ccaccggtat gtggagatcc
     5461 gatatctcgg tcgccatgtt ggggtggggg tcgatggcga tccaggggtg tttcctgagg
     5521 tgtttgaccg gctcgatcgg gaagtgagcg gcggcatcgg tccctatatt gatgaaggcg
     5581 tccacctcgt cccgcatggc gaggtcgacg gagctcgtct cgccggggtt catgtgggcg
     5641 atatcgcgtt tggtcaggtc caggcagtag gggaacccgt attgccatga ccagaccgcg
     5701 cccggcccgg tgatgttgta gtggccccgc atcgccatga tcgtccactt ggtgtagtcg
     5761 ttgaggtccc gggtcaggct gatggcgata tccacgttgt ggttccggcc gtcggagtgg
     5821 cagagcccca tgccgtagaa gatggtgccg aaccgggcct tcttcatgat attggcgacc
     5881 tcgaggatct gctcgcgctt gatccccgca acctcctcgg ggatctcgtg gccccggacg
     5941 acggcgcgga acgcgtcgaa gagttcgtag tcgtgcccct gcctcacctg caggtagatg
     6001 tccgccacct gggcggtatc ggtgtggcgg ggatcgatca cgatgatctt gcggcttttc
     6061 tggcctttgc ccgtgaagaa accgcggggg aagatcgagt agcgggacat gtgccgcggg
     6121 tgggcgtgag cggggtttgc gccccagtag aggacgacgt ccgcgcggtt ctttgtctca
     6181 ccgagggtgc agctcgggta gccgttgtcg aatatggcaa ggaacgagga cccgtggcag
     6241 atggacgcgc agttgtcgag cacggctcct gccttctcgg cgaccttggc ggcggcggcc
     6301 atgccttcgc agttggtcga cccgaacccg tagatcaggg gtttctttgc gttgtgcagg
     6361 acctttgccg tgtagtcgat cgcctcgtcg taggagatct ctttgaaggt gccgtccggc
     6421 tggcggaggc ggggcagagc gtgccggtct tccatggcac ggtggaagac cgtgctgccg
     6481 atgatgcagg cgttctcaac ctcaaggatc ttctttccgt catcagagac ggtgacgaca
     6541 aggtcgtcgc agcagacgcc gcagaacggg catccgacat tctcaatctt ttttgccatt
     6601 actgatcacc tttccacatc cctacggcgc tctggacgag ttcgagcgtg ctcttgcgcc
     6661 gttcgcccgg agcgacgggt tcgactgtca cgggaaagcc gctgtactgg ggttccccgg
     6721 tcgcctgggt acgcgccggg acgatctggt ttgcatagac gccctgcggg atgaacgcaa
     6781 gaccggggta gagcgcctgg cgccccctga cgctcttgac gaccacactt ccgcattcac
     6841 tcgtcacccg tacgagctgg tttgggacca gaccgagcgc tctgacgtcg tcgtcgtgca
     6901 tctcgatgat ggagcaggcc tcgaagtaat ccggggtcat cttgcccttt actatcccaa
     6961 cgccttcttc tacggagcgc tgggtgatca tgttcagcgt aatcttcttc gccatggttc
     7021 tgcctcgttg tgtacctgca ggaccggttc tcttcctgga gaaccggccc cggaacgtgg
     7081 tgctccggcc cggatgcggg ccgttcattc tgttgcgaga gcctcctccc cggggggcgc
     7141 ccctacccgg catgccgggc cttcctgcac ggggaaggtc tgccagaaac cgggggatac
     7201 gcagatcggc tgtccatcag gggttgactg agactctccc ggcggagcac cggagagatc
     7261 ggtttatcat aacttgtaat ggtttatatt taactattcc gcatttgtaa gtgatatcag
     7321 gcaaccagat ttagtttaaa ttgttaattt atcataatta acttataccg gccccggttg
     7381 cggtgacgtt gcagaaaaat cgcgaggtgg tattttcacc ggctgcccgg gtgcttccgg
     7441 tctgatatcc ggttaacccg gtcgggcggg gattggccgg cacaacatcc gccgcccggg
     7501 tgcatcgggg agattggccg gtgggaagac aggctacgga tgaggtttgc ggggttgatc
     7561 gccggtggag ccggctgcgg gttgctcccc ttctttctgg cgtgcggggg gctcactgca
     7621 ccgggccggg ttactccgcc cgccgatgga tagctcggac gtgggtcagg gagcccctgc
     7681 tcacgcggaa gatctctctt ggggggtgcc ccccgggccg gcggcctcga tcccttcccg
     7741 gggcgcagtg cggggcgata tcttcccgcc ctgcccgtgg cgtaccgggg atgggttgcc
     7801 ggcgggtaga tgctcaccgt atccgtttta ttcccgatcg aaatcaggcg cgtttgtaaa
     7861 ataggtgtct gacgattcgg ggtttcgaga aacctcccca ttttgagtag cattatatat
     7921 ctttgagtgt aatgtataca gtcaggtact tttgatctcc ttcatggagc aataatagca
     7981 catattgtaa ctttgtttag ctattttatt atttaataag tctctctctg ctgacctctc
     8041 ccggttgctg gtcgagggtg gcctctctcc aaaacccttt ttaagtacaa cgctcaacta
     8101 tctgagccat aaaccccctg ttttaaaata attcgatcag aataaaatct gggtaaatat
     8161 tattatttat gctaaaattc gatctaaatt cgtaaatttt attaataacc ctccttctat
     8221 tcttatagtc ccttctggga cgtgcagtag cggctttcag agaaccgggg catgtgaacg
     8281 ctccggcctc tttcacgaac ggaatgcatt gaacaagggt aaaaggatga aatactatgg
     8341 ctaaatacac ggaaacaatc gatctctatt ctgacgacgg gaagctgctc aagggcggcg
     8401 tcaccctcga cagaatcagc ccgctggtca acccggccac aagtaagatc atcgacctga
     8461 ccaagagaac gatcaacgtg aaccttggag gcatccagga cgcacttaag accggaaaac
     8521 tcgggaaggg caagatcaag ggaagagagc tcgacctccc catcatggag aacaaggacg
     8581 ccatcgtcgc ccggatcaag gacatgatcc aggttaccga gggcgacgac accgagatca
     8641 tggagttcaa cggtggaaaa ctccagctcg tcaaggtccc ggcaaagcgc ctggcaaacg
     8701 ccgccactta cgacgccgcg atcacgtcgg tcgcagccgc gaccacctac gcgatcgtcg
     8761 aggagttcgg catcgacgcc ttcaacgcat ccaccgtcaa ggcagcctgc tggggcggct
     8821 acccgcacac gcaggacctc gagggtgcgc tcgtcacgtc gatcctcaac atcccccaga
     8881 acaacgaagg cctcggctac gcgctccgga acatcccggt caaccacacc gtcatgatga
     8941 ccggcaggaa cgccctgcag ggcgctgcac ttgcatcgac gcttgagacc gccggcgtct
     9001 ttgagatggg aggcgccgtc gggccgttcg aacgcgccat gctgcttgcc tacgcctacc
     9061 agggcctgaa cgcgaacaac atggtctacg acctcgtcaa ggcgaacggt gcgaccggaa
     9121 ccatcggcac ggtcgtccag agcctggtcg agcgcgccat cgaggacaag gtcatcaccc
     9181 cgggcaagaa gggcgggtac ttccagttct acgacaccaa ggaccccatg ctctggaacg
     9241 cctacgccgc tgcgggaacc atggcagcca ccatcgtcaa ctgtggtgcc ggacggttcg
     9301 cccaggcggt ctccgcgacg ctcctctact tcaacgacct gcttgagcac gagaccggtc
     9361 tcccgagcac cgactacggc cgtgtcatgg gtaccgccgt cgggttctcg ttcttcagcc
     9421 actcgatcta cggcggcggc ggccccggta tcttcaacgg caaccacgtc gtgacccggc
     9481 acgcaaacgg cgtcgccatc ccgtgcgtgg tcgctgcagc ggcgctcgac gccggaacgc
     9541 agatgttctc gcccgaaagc acatcgaaga tcttcgccga cacctacggc aagatcgacg
     9601 tcttcaacaa gccgatcgac cagatcgcaa acggagcctg aacgagcgga gatccgaatg
     9661 acgaacgccg catctccgca acccgggcct atcgggcacc gatccggagc ttcccgtgcg
     9721 gtgcaccgcc tatcggcctt gcggaccccc ggtacgggga aggtccggcg gtcacccgga
     9781 gatatggcag aaagtcgttc aaatcaaccg aaaatggata cgataacaat caatctgagg
     9841 tgaatgatac aatggcatac aagccccagt atggtcccgg gacgtcgatt gtcgccgcaa
     9901 accggcgcaa gcagatgaac cccggccaga agcttgagaa ggttcgttcc gtaacggatg
     9961 aggacatcgt gctgatcctc ggccaccgtg cccccggctc ggcatacccg agcgcccacc
    10021 cgccgctcgg tgagcagcag gagcccgact gccccatgcg caggatcgtc aagcccaccg
    10081 aaggcgccag tgccggcgac cgcgtccgct acatccagtt cgccgactcg atgttcaacg
    10141 ccccctcgca gccctaccag cggacctaca ccgagatgta ccgcttccgc ggcatcgacc
    10201 ccggcacgct ctccggccgc cagatcgtcg agtgccgcga gcgcgacctc gagaagtacg
    10261 caaaggaact catcgagacc gaactcctcg acccggcccg caccggcatc cgcggtgcga
    10321 ccgtgcacgg ccactcgctc cgtcttgctg aagacggcat gatgttcgac atgctgcaga
    10381 ggaacatcct cggcgaggac ggcgtcgtcc gttacgtcaa gaaccagatc ggcgagcccc
    10441 tcgaccgtgc cgtcgccgtc ggcaaaccgc tcgacgagaa gtggctcaag gcccacacga
    10501 cgatcttcca ctcgctcgta gggactccct accgtgacga caccgagtac atcgaatacg
    10561 tccagcgcat ccactcgctg cggacgaaat acggcttcat gcccagagaa gagtgatcac
    10621 aatggcaaag attgagagat cccagaagct gttcctgaaa tccctcaagg agaagttcca
    10681 gggacaggac gtcgagtccg agaccgccga gttctacaag ttcaacggtg ttcgccagtc
    10741 tcctcgcaag gtggagttca tgaaggcgag ccttgccgcc gagatggacc gcggcatatc
    10801 catgtacgac cccgagcgct gccacctggg cggtataccg atgggccagc gccagctgat
    10861 gacctacgag gtcagcggca ccggcgtctt cgtcgagg
//
LOCUS       DAOSEE010000075        10834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_2327_length_10834_cov_230.786065, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000075 DAOSEE010000000
VERSION     DAOSEE010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 10834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10834
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2327_length_10834_cov_230.786065"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..336
                     /locus_tag="PLI76_06900"
     CDS             55..336
                     /locus_tag="PLI76_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HOI61796.1"
                     /translation="MTEEDIEATRAEAKAYAKEKGYVLNVDEKQLSAVLRGLARNKER
                     LGAAYCPCRLRSGDPEKDRIIVCPCLYHEQEIEEQGSCHCRLFFKKKAE"
     gene            complement(407..2056)
                     /locus_tag="PLI76_06905"
     CDS             complement(407..2056)
                     /locus_tag="PLI76_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HOI61797.1"
                     /translation="MVTRLRRYRQIADVLAKYGFGILVEEVFPGIGRLWKAPVPAEEK
                     QSVYERIRLALEELGPTYIKFGQIMSTRRELLPPELIAELQRLQDRVDPLPFEEIRPV
                     IRKYCPNLEECFDIIEADPVAAASLSQVHRAVMKDGQVVALKVQRPGIVDLIEADILI
                     LQSLARRVESLFPDLRVYNLPGMVDEFAVQIRRELDFSQDGMNAERLRRNMRDIPCVK
                     IPRIHWGITGPFLLAMDYVEGVRIDDVAAIRAFGLFPEEIADIGFQAYVQQIFVDGFF
                     HGDPHPGNLFVTEQGEVVFLDYGIVGVLRPERKRMFIDLLLAMTRTDVAGVVSALERL
                     DVRIRPSDLDAVKDDLYLVLLDYREMSLERMNFATALGGLTDTLRRYHIRVPPTLMLM
                     MKVIIMVMDIGTRLDPAFNFDRRIHPYLTDIVMQERFSPETMANAVRSLASAAEGLLA
                     IPENVNDTLKTLSEGTVTIDLEDTDLKRITDVIDQTSDKVIVGLVVAAVVVGSSLVLR
                     VTELPVPGYVTTLAVLGYVFAVLFGFYAVYNALRHGHVLRR"
     gene            complement(2103..3272)
                     /locus_tag="PLI76_06910"
     CDS             complement(2103..3272)
                     /locus_tag="PLI76_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI61798.1"
                     /translation="MTFDTKPVWGLSAAHLVTDLYSPVLPAILPLLIAEQGYSFFLAG
                     LIVTVYNLTSSLIQPLIGWAFDTRGFAVHVGTSVAIGAIFISIVGLLNNYTLVLASVA
                     VAALGHAFFHPSALGTVSRLTRDASRGRITSYFVIGGNLGYAIGPICAGVAVGLMGLP
                     GLVVLAVPGLVMAAVLRQVLPAPDRLKAAPAADPGKRPAYRAIALLVTASGLRAWAIF
                     GSVAYLPTVLTLRGIDLVTANLLVSGMLIAGVVGQLVGGALSDRYGRKEYTILGLVTA
                     IPPFVVFLSTGGILSLVALMIFGFILWSTFAVTVAMGHEIAPGNVGLISGLMLGLAVG
                     VGGMGVAITGWIADMTSLSLGLMTIPLAIGLALPLFLAVPYPWKSLSQRRSPVPG"
     gene            complement(3277..3888)
                     /locus_tag="PLI76_06915"
     CDS             complement(3277..3888)
                     /locus_tag="PLI76_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="METTL5 family protein"
                     /protein_id="HOI61799.1"
                     /translation="MNLRHLEMRLERLEGFERPIARLEQYQTPAPVAARLLHHAAMQG
                     AIEGRRVCDIGCGTGILACGAALLGASAVAGVDIDPAAIDIAHRNARALGVSVEFLVA
                     DIRDPTVDRTRLACDTVVMNPPFGAQKAHADRPFIDRALDLADVVYGIFNEGSTPFVA
                     AYTEGRAAIEEVIRCAFPLRRTFAHHRKERVDITVEVIHLRRI"
     gene            complement(3885..4838)
                     /gene="dph2"
                     /locus_tag="PLI76_06920"
     CDS             complement(3885..4838)
                     /gene="dph2"
                     /locus_tag="PLI76_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043364 - glycyl-radical enzyme activating
                     activity [Evidence IEA];
                     GO_process: GO:0017183 - peptidyl-diphthamide biosynthetic
                     process from peptidyl-histidine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthamide biosynthesis enzyme Dph2"
                     /protein_id="HOI61800.1"
                     /translation="MSLIPVSDLTAKLHERGARSVALQFPAGLARQAPQVAAALRDAG
                     FFVLVSGDPCYGACDLALDTLRHADVLVHFGHAPVEDRPGVIYEPVRFDFDVAVLDRA
                     LPLLSTRRIGLVTTVQHIHLVDAMAAYLREHGIEAAVAPGDGRTPFPGQVLGCNFAAA
                     RATGADEILFVGTGVFHPVGIRLATKKRVVALDPYTGEAGEVDADRLVRRRAAVMERA
                     RGAANFGIIVSTKSGQQRMDLARRLAALSDRAVLVMMREVSPGELLDLGFAAYVNTAC
                     PRLAYDDQARFPAPVLAPPEFEILCGARSWDDYTIDEYLSP"
     gene            complement(4816..5367)
                     /gene="hpt"
                     /locus_tag="PLI76_06925"
     CDS             complement(4816..5367)
                     /gene="hpt"
                     /locus_tag="PLI76_06925"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine/guanine phosphoribosyltransferase"
                     /protein_id="HOI61801.1"
                     /translation="MLERLIQSLEASPVMRRGEYNYFIHPITDGVPLLEPVILREIGC
                     AMVRILDLAGVDKIVVCEAMGIPIGVALSMMTDIPLVIVRKRPYNLPGEVAVHQATGY
                     SKGELYLNGVGAGDRVVVIDDVCSTGGTLAALVSALEQVGADIADICVVIGRGDVDLG
                     RPLKTLVRIEVSEDRVHVVDTCL"
     gene            complement(5360..6457)
                     /gene="mfnA"
                     /locus_tag="PLI76_06930"
     CDS             complement(5360..6457)
                     /gene="mfnA"
                     /locus_tag="PLI76_06930"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase MfnA"
                     /protein_id="HOI61802.1"
                     /translation="MREQGCPEGDLFSFLSLQRKKDLGYRNILSSMCTPPHPVAARAH
                     AMFLETNLGDPGLFPGTAELEELLVRRLATLMHHPKAGGYATSGGTESNIQAFRIAKK
                     QKPVESPNVVVPASSHFSFLKACDILGLAMRTAPLDTHFRVDAEAVESLVDKDTVCIV
                     GIAGTTEYGTVDPIAHLADVALDRDVFLHVDAAFGGMVVPFLDQPIPFDFHLPGVDSL
                     SVDPHKMGMSTIPAGCLLVRNPEQFSCLNVDTPYLTVKRECTLAGTRPGASVAAAFAV
                     LEYLGMDGMRAVVAGCMENTRRLVEGMETLGCERAVTPDVNVATFSCSRAPAGWRVST
                     TRAGHMRVVCMPHVTRDVIEAFLKDMSDMHA"
     gene            complement(6450..8738)
                     /gene="ppsA"
                     /locus_tag="PLI76_06935"
     CDS             complement(6450..8738)
                     /gene="ppsA"
                     /locus_tag="PLI76_06935"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HOI61803.1"
                     /translation="MKEMPNILWLEEIKKEDIPSVGGKGASLGEMTAIGLPVPKAFVV
                     TAQAFRKFLVETGIEETLFRRLARLDVDDNGALESVSRDVQDLVLSVDIPDHFREEIT
                     DAYARMGANGMVVAVRSSATAEDLPEASFAGQQETFLNILGDEDLINAVHRCWASLYG
                     ARAIYYRAKQGFDDRSVNIAVVVQELIRSEKSGVMFTSHPVTGEPLTIIEGSWGLGEA
                     VVSGSVSPDNYVFDLRTERVVDRLIAEKEIMIVPEGEHGTTIVNLSGRERTAPVLSDA
                     EVARLATLGKIAESHYGVPQDVEWAIVGDDVFILQSRPITTIRRPEIPRPGTAPAPRD
                     KPGVILLEGQGASPGVASGRVVIVRDVKDTSAVKEGDILVTKMTNPDMVPAMRRVSAI
                     VTDEGGMTCHAAIVSRELGTPAVVGTKKATKVLQDGQIITVDGEKGTIYEGATAAAAP
                     APAVASAAAAAPAPLITGTLVKVNVSLPEAAKRAAATGADGVGLLRIEHLILGLNKTP
                     GWYVEHNREEEFVGELYGGIKTVLDAFPGKPVWVRTLDAPTDEFRNMKGGEEEPIEHN
                     PMLGWRGIRRDLRSPDQFRLQVEAFKRLWAAGYSNLGVMFPMVNHPGEFIQARAMMKD
                     WGVDVENATLGVMIEIPSSAILVEDFIKAGIRFASFGTNDLVQYTLAIDRNNEHVADM
                     YEPEHPAVLWLIDYAIKACREHGIECSICGQAGSDPAMVAWLVGHGITSVSANIDAVP
                     RIREAVARKERQILLDAARRNA"
     gene            8834..10417
                     /gene="serA"
                     /locus_tag="PLI76_06940"
     CDS             8834..10417
                     /gene="serA"
                     /locus_tag="PLI76_06940"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOI61804.1"
                     /translation="MRYRVLVSDPLAEEGIDILKGFCDVDINTGLTEDELVAAIGGYD
                     ALLVRSGTEVTARVIDAGARLKFIGRAGAGVDNIDTEAATRRGIIVANAPEGNTLAAT
                     EHTMAMMLSLARNIPQANASLKKGEWKRSKFIGIELNDKVLGIVGLGRIGNEVAKRAR
                     AMEMKCIAYDPFISKERAAKIGVELVSLDDLFRRADVITVHTPLIKETRHIVNAKTIA
                     TMKDGVRLINCARGGIIDEKALADAIASGKVAGAAVDVFESEPPTDSPLIGLDHVIVT
                     PHLGASTVEAQMNVAISVANQCISVLEGGPAKYVVNAPMVPAEQQALVEPYAVLAQKM
                     GRLLIQLIEGRLESIEITYGGEVAQMPNTKFITRVILKGMLDPILQTPVNIVNAEFVA
                     KERGIRMSETTTEEAQGFRNIICITAKTDRMTETVSGSVSGPNRARIVSIGGYMTDLT
                     PTGHVVISRHTDKPGVIGKAATILGRVNVNIAGMQVGRHRPGEEALMVLTVDSSVPAD
                     AMDEIRKIDGIHTAKHAEI"
     gene            10554..10627
                     /locus_tag="PLI76_06945"
     tRNA            10554..10627
                     /locus_tag="PLI76_06945"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10588..10590,aa:Val,seq:gac)
BASE COUNT         2023 a         3390 c         3623 g         1798 t
ORIGIN      
        1 cttgatgaca acccatatcg aagagtacgg acggcaggag gcctgattaa gagtatgacc
       61 gaagaggata tcgaggcgac gcgcgcggag gcgaaagcct acgcaaagga gaaaggttac
      121 gtcttgaacg tcgatgaaaa gcagctctcg gccgttcttc gcgggcttgc ccggaacaag
      181 gagcggctgg gggcggcata ctgtccctgc aggctccgga gcggcgatcc cgagaaagac
      241 cggatcatcg tctgcccctg cctctaccac gagcaggaga tcgaggagca agggtcctgc
      301 cactgcaggc tcttcttcaa gaagaaagcc gagtgaaccg cccccggccc ggatgatctt
      361 tttttgggcc ggcaggctct ccgcgttgag cgggaggggg gaccggtcag cgccggagga
      421 cgtggccgtg cctcagggcg ttgtagaccg cgtagaaccc gaacagcacc gcgaagacgt
      481 agcccaggac ggcaagcgtc gtgacgtagc ccgggaccgg gagctcggtc acccggagca
      541 ccagtgagga ccccacgacg accgcggcga ccaccagccc gacgatgacc ttatcgctcg
      601 tctggtcgat gacatcggtg atccttttaa gatcggtatc ctcgagatcg atcgtgacgg
      661 tcccctccga gagggttttc aaggtatcgt tcacgttctc cggtatggca agcagcccct
      721 ccgcagcgct tgcaagcgac cgcaccgcat ttgccatggt ctcgggggag aaccgctcct
      781 gcatcacgat atccgtcagg tagggatgga tccgccggtc gaagttaaac gccgggtcga
      841 gccgggtgcc gatatccatc accatgatga tcaccttcat catcagcatc agggtgggcg
      901 gaacccggat gtggtagcgc cggagggtgt cggtcaggcc cccgagcgcc gtcgcgaagt
      961 tcatccgctc aaggctcatc tcccggtagt cgagcaagac caggtaaagg tcatctttga
     1021 cggcatcaag gtcggagggc ctgatgcgca cgtctagcct ctcaagcgcc gagaccacgc
     1081 ccgccacatc cgtccgggtc atggcgagga ggagatcgat gaacatacgc ttcctctccg
     1141 ggcggaggac gccgacgata ccgtagtcga ggaagacgac ctcgccctgc tccgtcacga
     1201 agaggtttcc ggggtgcggg tccccgtgga agaacccgtc gacgaatatc tgctggacgt
     1261 aggcctgaaa ccctatatcg gcgatctcct cggggaagag gccgaacgcg cggatggccg
     1321 ccacgtcgtc gatccggacc ccctcgacgt aatccatggc gagcaggaag gggccggtga
     1381 tcccccagtg gatccgcggg atcttcacgc acgggatgtc gcgcatgttc ctcctgagac
     1441 gctccgcgtt catcccgtcc tgggagaagt ccagctcgcg ccggatctgg accgcgaact
     1501 catccaccat ccccggcagg ttgtagaccc gcaggtccgg gaagagcgac tccacccgcc
     1561 gggcaagcga ctgcaggatg aggatatcgg cctcgatcag gtcgacgata ccggggcgct
     1621 gcaccttgag ggcgacgacc tggccatcct tcatcacggc ccggtgcacc tgcgagaggg
     1681 aggccgccgc gacgggatcg gcctcgatga tatcgaaaca ttcctcgagg ttcggacagt
     1741 acttccggat cacgggccgg atctcctcaa acgggagcgg gtcgacccgg tcctgcaacc
     1801 gctgcagctc cgctatcagc tcggggggga gaagttcccg ccgtgtactc atgatctggc
     1861 cgaacttgat gtaggtcggc cccagctcct cgagcgcaag gcggatgcgc tcgtacaccg
     1921 actgtttctc ttcagcgggc acgggggctt tccacagccg cccgatcccg gggaagacct
     1981 cctcgaccag gataccgaaa ccgtacttcg ccagcacatc cgcaatctgc cggtagcgtc
     2041 ggagccgggt gaccatcggg gatggactct gcactcccgg tacttaagag ggtggggtgg
     2101 ggttatcccg ggacggggga ccgcctctgc gagagcgact tccagggata ggggacggcg
     2161 aggaagaggg ggagggcaag gccgatcgcg agcgggatcg tcatgaggcc gagcgagagc
     2221 gacgtcatat cggcgatcca cccggtgatc gcgacaccca tgccgcccac gccgacggcg
     2281 agcccgagca tcaggccgga gatgagcccg acgttccccg gggcgatctc gtgacccatg
     2341 gcgacggtga cggcgaaggt cgaccagagg atgaacccga agatcatcag ggcgacgagc
     2401 gagaggatgc cgccggtcga gaggaagacg acgaacggcg ggatggcggt aaccagcccg
     2461 aggatcgtgt actccttgcg gccgtagcga tccgagagcg cgccgccgac gagctgcccc
     2521 acgaccccgg cgatgagcat ccccgagacg agcaggtttg cggtcacgag gtcgatcccc
     2581 cgcagggtga ggacggtcgg gaggtaggcg accgaaccga atatcgccca tgcccggagg
     2641 cccgaagccg tgaccaggag ggcgatagcc cggtaagcgg gccgcttgcc cgggtcggct
     2701 gccggcgcgg ctttcaggcg atccggcgcg gggagaacct gccggaggac cgccgccatc
     2761 acaaggccgg ggacggcgag tacgacgagg ccgggaagac ccatgaggcc gaccgccacc
     2821 ccggcgcata tggggccgat cgcgtagccg aggtttccgc cgatgacgaa gtaggaggtg
     2881 atccgacccc ggctcgcgtc ccgggtcagg cggctgacgg tcccgagcgc gctcgggtgg
     2941 aagaaggcgt gccccagcgc cgctacggcg acggatgcaa ggacgagcgt gtagttgttg
     3001 agcaacccga cgatggatat gaagatcgcg cctatggcaa cgctcgtccc gacgtggacg
     3061 gcaaaccccc gggtatcgaa ggcccagccg atgagcggtt gtatgaggga cgacgtgagg
     3121 ttgtagacgg tgacgatcaa ccccgcgagg aagaacgagt agccctgctc cgcgatgagg
     3181 agcggcagga ttgcagggag aaccggcgag tagagatcgg tcaccaggtg ggcggcggag
     3241 agcccccaga cgggtttggt atcgaaagtc acggtattag atccgtcgta aatgtatgac
     3301 ctctacggta atatccactc gctctttgcg gtggtgggca aacgtccgcc ggaggggaaa
     3361 cgcgcaccgg atcacctcct cgatcgccgc ccgcccttcg gtgtaggccg ccacgaacgg
     3421 tgtcgagccc tcgttgaaga tgccgtagac cacatccgca aggtcgagcg cccggtcgat
     3481 gaacggccgg tcggcatgcg ccttctgcgc cccgaacggc ggattcataa cgacggtgtc
     3541 gcacgcaaga cgtgtccggt cgacggtggg atcgcggata tcggcgacga gaaactccac
     3601 ggagacgccg agcgctcgcg cgttccgatg ggcgatatcg atcgcggccg gatcgatatc
     3661 cacccctgcc acggccgagg cgccgaggag cgccgccccg caggcgagga tccccgtccc
     3721 gcacccgatg tcgcagaccc ggcgcccctc gatggcgccc tgcatggcgg cgtggtgaag
     3781 gaggcgggcc gccaccggcg ccggggtctg gtactgctcg aggcgggcga tcggccgctc
     3841 gaaaccttcg aggcgctcaa ggcgcatctc aaggtgccgg aggttcatgg ggagaggtac
     3901 tcgtcgatgg tatagtcgtc ccaggaccgg gccccgcaga gaatctcgaa ctccggcggc
     3961 gccagcaccg gggcggggaa cctggcctgg tcgtcgtagg cgagccgcgg gcaggcggta
     4021 ttcacgtagg ccgcaaaccc aagatcgagg agctcgccgg gcgatacctc gcgcatcatg
     4081 acgagcaccg cccggtcgga gagggcggca agccttcgcg cgagatccat cctctgctgt
     4141 ccgctcttcg tgctgacgat gatcccgaag ttcgcggcgc cgcgggctct ctccatcacc
     4201 gcggcgcggc ggcggaccag gcggtcggca tcaacctccc ctgcttcgcc cgtgtatggg
     4261 tcgagcgcca ccacccgctt tttggtcgcg agccggatgc cgacaggatg aaagaccccc
     4321 gtcccgacaa agaggatctc gtccgccccc gtcgcccgtg cggcggcaaa gttgcacccg
     4381 agcacctgcc cgggaaacgg cgtgcggcca tcgccgggtg cgacggccgc ctcgatgccg
     4441 tgctcgcgga ggtaggccgc catcgcgtcg acgaggtgga tgtgctggac ggtcgtgacg
     4501 aggccgatcc ggcgggtgga gaggaggggg agggcgcggt cgagcacggc cacgtcgaag
     4561 tcgaaccgga cgggttcgta tatgacgccc gggcggtctt cgaccggcgc gtggccgaaa
     4621 tggaccagaa cgtcggcgtg ccggagggtg tcgagcgcaa ggtcgcaggc accgtaacag
     4681 gggtcgccgc tgacgagcac gaagaaccct gcgtcgcgga gggcggcggc cacctgcggc
     4741 gcctgccgcg caagcccggc ggggaactgg agggcgacgg accgcgcccc gcgttcgtgg
     4801 agttttgcgg tgaggtcaga gacaggtatc aacgacatgc acccggtcct cggagacctc
     4861 gatcctgacc agggtcttga gcggccggcc gagatccacg tcgccccggc cgatgaccac
     4921 gcatatgtcg gcgatatcgg cgcccacctg ttcgagcgcg ctgacgaggg cggcaagcgt
     4981 ccccccggtg ctgcagacgt cgtcgatgac cacgacccgg tcgcccgcgc cgaccccgtt
     5041 caggtagagc tccccctttg agtagcccgt cgcctggtgg accgcaacct ccccgggaag
     5101 gttgtagggg cgttttcgga cgatcacgag cggtatatcg gtcatcatcg agagggcgac
     5161 gccgatgggg atccccatcg cctcgcagac gacgatctta tcgactcctg caaggtcgag
     5221 gatccgcacc atggcgcacc cgatctcgcg gaggatgacc ggctcaagga gcggcacccc
     5281 gtcggtgatc gggtgaatga agtaattgta ctccccgcgc ctcatgaccg gggaggcctc
     5341 cagcgattgt atcaggcgtt caagcatgca tatcactcat atctttaaga aacgcttcta
     5401 tcacgtcgcg ggtgacgtgc ggcatgcaga cgacccgcat gtgcccggcc cgggtcgtcg
     5461 agacccgcca gccggcaggc gcgcggctgc aggagaacgt cgccacgttg acgtcggggg
     5521 tcaccgcccg ctcgcagccg agcgtctcca tcccctcgac cagtctccgg gtgttctcca
     5581 tgcaccccgc gaccaccgcc cgcatgccgt ccatccccag gtactcgagg accgcgaacg
     5641 cggcggccac cgaagccccg ggccgggtgc cggcaagggt gcactcccgc ttcaccgtca
     5701 ggtagggcgt atcgacgttg aggcacgaga actgctccgg gttccggacg aggaggcatc
     5761 ctgccggtat ggtgctcatc cccatcttgt gggggtcgac ggagagggag tcgacacccg
     5821 ggagatggaa gtcgaagggg atcggctggt cgaggaacgg caccaccatg ccgccgaacg
     5881 cggcgtcgac gtggaggaag acgtcccggt caagagccac gtcggcgagg tgggcgatgg
     5941 ggtcgaccgt accgtactcc gtcgtcccgg ctataccgac gatgcagacc gtatccttat
     6001 ccacgaggga ctcgaccgcc tccgcatcca cccggaaatg ggtgtcgagc ggcgccgtcc
     6061 gcattgcaag cccgagtata tcgcaggcct tcaaaaacga aaaatggctc gatgccggga
     6121 ccacgacgtt tggagactcc accggcttct gcttctttgc tatccggaac gcctgtatgt
     6181 tcgactccgt tccgccgctc gtcgcatatc ccccggcctt cggatggtgc atcagggtgg
     6241 cgagcctccg gacgaggagt tcctcgagtt ctgcagtccc cgggaagagg ccggggtcgc
     6301 cgaggttcgt ctcgaggaac atggcatgcg cccgggccgc gaccgggtgg ggcggcgtgc
     6361 acatcgaact cagtatattc cggtagccga gatccttctt tcgttgtaat gaaaggaagg
     6421 agaagaggtc cccttcgggg catccctgct cacgcatttc ttctcgccgc atccaacagg
     6481 atctgtcgct ctttacgcgc aacggcctca cggatgcggg gaacggcatc gatgtttgcc
     6541 gagacgctcg tgatgccgtg cccgacgagc catgcgacca tagcggggtc ggagccggcc
     6601 tggccgcata tggagcactc gatgccgtgc tcgcggcatg ccttgatcgc gtaatcgatc
     6661 agccagagca cggcagggtg ctcgggctcg tacatatcgg cgacgtgctc gttgttccgg
     6721 tcgatggcga gcgtgtactg gacgaggtcg ttcgtcccga acgaggcaaa ccggatgccg
     6781 gccttgatga agtcctcgac cagtatggcg ctgctcggga tctcgatcat gacgccgagg
     6841 gttgcgttct ccacgtcgac cccccagtct ttcatcatcg cccgggcctg gatgaactcg
     6901 ccggggtggt tgaccatcgg gaacatcacg ccgaggttgc tgtagcctgc ggcccagagc
     6961 ctcttgaacg cctcgacctg caggcggaac tggtcggggc tccggagatc ccggcggata
     7021 ccgcgccacc cgagcatcgg gttgtgctcg atgggttctt cttccccgcc tttcatgttc
     7081 cggaactcgt cggtcggagc gtcgagcgtc cgaacccaga cgggttttcc ggggaacgca
     7141 tcgagcaccg tcttgatgcc gccgtagagt tcgccgacga actcttcctc cctgttgtgc
     7201 tcgacatacc agcccggtgt cttgttcagc ccgagtatga ggtgctcgat gcggaggagg
     7261 ccgaccccgt cggcgccggt ggcggccgcc cgctttgcag cctcggggag ggagacgttc
     7321 accttgacga gcgtgccggt gatgagcggg gcgggcgccg ccgctgccgc cgacgcgact
     7381 gccggcgccg gtgccgctgc tgccgtcgct ccctcgtaga tggtgccctt ctcgccgtcg
     7441 acggttatga tctgcccgtc ctggagcacc ttcgttgctt tcttggtccc gacgaccgcg
     7501 ggcgtcccga gttcgcggct cacgatggcc gcgtggcagg tcatgccgcc ctcgtcggtg
     7561 acgatggcgc tgacccgccg catcgccggc accatgtcgg ggttggtcat cttcgtgacc
     7621 aggatgtcgc cttccttgac ggcgctcgtg tccttgacgt cgcgcacgat aacgacccgg
     7681 ccgctcgcga cgccggggga tgcgccctgg ccctcgagga ggatcacgcc gggcttatcc
     7741 cggggtgccg gtgcggtccc cgggcggggg atctccggcc gccggatcgt cgtgatggga
     7801 cgtgactgga ggatgaagac gtcgtcgccg acgatcgccc actcgacgtc ctgcgggacg
     7861 ccgtagtggc tctcggcgat cttgccgagc gtcgcgaggc gtgccacctc ggcgtccgag
     7921 aggaccggag cggtgcgctc tctcccggag aggttgacga tcgttgtccc gtgctcccct
     7981 tccggcacga tcatgatctc cttctcggcg atcaggcggt cgaccaccct ttctgtgcgc
     8041 aggtcaaaga catagttgtc gggcgagacg ctgcccgaga cgacggcttc tccaagcccc
     8101 caggaaccct cgatgatcgt caggggttcg ccggtgacag ggtgggaggt gaacatgacg
     8161 ccggacttct ccgaccggat gagctcctgc acgacgacgg cgatgttcac gctccggtcg
     8221 tcgaacccct gtttggcccg gtagtagatg gcacgcgcgc catacaggga ggcccagcac
     8281 cggtggactg cgttgatgag gtcttcgtcg ccaagaatat tgagaaaagt ctcctgctgg
     8341 ccggcaaaac tggcttccgg caggtcttcg gcggtggcgc tcgaacggac ggcgacaacc
     8401 attccgttcg cccccatccg ggcgtatgcg tcggtgatct cctcgcggaa gtggtcgggg
     8461 atatcgacgc tcaggacgag atcctgcacg tcacgggaga cggactccag tgcaccgttg
     8521 tcgtcgacgt cgaggcgcgc cagacgccgg aagagcgtct cctcgatccc ggtctcgaca
     8581 aggaacttgc ggaacgcctg cgcggtcacc acaaacgcct tcggcacggg gaggccgatt
     8641 gccgtcatct caccaagaga ggctcccttt ccccctacgg aggggatatc ttcctttttt
     8701 atttcttcga gccacagaat gttgggcatt tccttcattc tcgcaccaca catatacttc
     8761 tcaccattac ataaaagatt tcggaagaaa aagcatcaat acacatgatg tcgaatcaga
     8821 tatgggttga actgtgagat atagagtgct ggtcagcgac ccgctggcag aggaagggat
     8881 tgatatcctc aaggggttct gcgacgtgga tatcaatacc gggttgaccg aggatgagct
     8941 cgttgccgct atcgggggct acgacgccct tctggtgcgt tcgggcaccg aggtgacggc
     9001 gcgggtcatc gatgcgggag cgagactgaa gttcatcggt cgtgccggcg ccggggtcga
     9061 caatatcgat accgaagcgg cgacacgccg gggcatcatc gtcgcgaacg ctccggaagg
     9121 caataccctg gcagccacgg agcatacgat ggcgatgatg ctctcgcttg cgcgcaacat
     9181 cccccaggca aacgcatcct tgaagaaggg cgaatggaaa cgctcgaagt tcatagggat
     9241 cgagttgaac gacaaggtcc tcggcatcgt ggggctcggg cggataggca acgaagtggc
     9301 gaagcgggca cgggcgatgg agatgaagtg catcgcctac gaccccttca tcagcaagga
     9361 gagggcggca aagatcggcg tcgagctggt ctcgctcgac gacctcttcc ggcgggctga
     9421 cgtcataacg gtccatacgc cgctgatcaa ggagacgcgc cacatcgtca acgcaaaaac
     9481 catcgccacg atgaaggacg gggtgcggtt gatcaactgc gcccgcggcg ggatcatcga
     9541 cgagaaggcg ctcgcggatg cgatcgcgag cggcaaggtt gccggcgccg cggtggatgt
     9601 ctttgagtcg gaaccgccga cggactctcc gctcatcggc ctcgaccacg tgatcgtgac
     9661 gccgcacctc ggggcgagca cggtcgaggc gcagatgaac gtcgcgatct cggtagcaaa
     9721 ccagtgcatc agcgtgcttg agggcggtcc ggcgaagtac gtggtgaacg cgccgatggt
     9781 cccggcggag cagcaggcgc tggtcgagcc ctatgcggtg ctcgcgcaga agatgggccg
     9841 cctcctcatc cagctcatcg aggggcgtct tgagtcgatc gagatcacct acggcggcga
     9901 ggtcgcccag atgccgaaca cgaagttcat cacccgcgtc atactgaagg ggatgctcga
     9961 cccgatcctg cagacgccgg tcaacatcgt gaacgcggag ttcgtcgcaa aggagcgcgg
    10021 catccggatg agcgagacca ccaccgagga ggcgcagggg ttcaggaaca tcatatgcat
    10081 cacggcaaag accgaccgca tgaccgagac ggtgagcggg agcgtctctg gcccgaaccg
    10141 cgcgcggatc gtgagcatcg ggggctacat gacggacctg accccgaccg gccacgtggt
    10201 gatctcccgc cacaccgaca aaccgggcgt catcgggaag gccgcgacga tcctcggccg
    10261 ggtgaacgtg aacatcgcag ggatgcaggt gggacgccac cggcccggcg aggaggcgct
    10321 gatggtcctc acggtcgact cctcggtgcc ggccgatgcg atggacgaga tccggaagat
    10381 cgacggcatc cacacggcaa agcacgccga gatctgatcg gtcccggccg atcacccccg
    10441 gttctcccgc gtgccggccc ccgatggggc ggagccggca cacgggggat ttccggggcg
    10501 agggaataat tttatacccg gagcaccaac cttattgatc gtcaggcaga cacgggctcg
    10561 tggtctagct ggttatgacg tcgccttgac atggcggaga tcctgagttc gaatctcagc
    10621 gagcccatca ccgtttttga aacaagttct tatacgaagc cttttaccag gagagactcc
    10681 gctcgaacga gctgataatc gtgctggaga catacgagcg agatcctcga gcatgaaaca
    10741 atcctcctcg ccttcatgca gcctcaacca ggtactacat ggggcctggt ttgggaatct
    10801 tacaccgacg agagtggggg atcttacacc gacg
//
LOCUS       DAOSEE010000076        10825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_2331_length_10825_cov_227.511328, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000076 DAOSEE010000000
VERSION     DAOSEE010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 10825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10825
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2331_length_10825_cov_227.511328"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PLI76_06950"
     CDS             complement(<1..241)
                     /locus_tag="PLI76_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOI61805.1"
                     /translation="MLDVRALSLSLGEFSLDGVDLTVGAGEYLIILGPTGAGKTILLE
                     TIAGIYAPASGTISLDGREITRTDPKDRGIGMVYQD"
     gene            complement(241..1053)
                     /locus_tag="PLI76_06955"
     CDS             complement(241..1053)
                     /locus_tag="PLI76_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI61806.1"
                     /translation="MIRDRSALPHPSPFKALFLAVLFGLVTYMVVVIVGLVLYPPLPA
                     LIESLTSPEILFAIRLSLITSAISTALCVVVAVPVAYSLARFSFPGKKAVNAVFNIPL
                     ALPPLVAGVALLIFYGPSKFGQMLTAAGFDIIYTPLGIIIAQFFVNLPYMVRVTRSAF
                     ETINPRYEHVARTLGCTEWGTFCQVTLPLARAGLIAGLVITWSKSIGEFGAVLMLAGA
                     TQMKTETLPIALFLNMSTGDLDLAIAASVILIVISVTSLAIFERYTDGRGVF"
     gene            complement(1050..1547)
                     /gene="tsaA"
                     /locus_tag="PLI76_06960"
     CDS             complement(1050..1547)
                     /gene="tsaA"
                     /locus_tag="PLI76_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HOI61807.1"
                     /translation="MNGADYFCKAIGIARTPFSDPAETPIQSVFSTARGTVEVYPGFE
                     DGLIALAGFSHIILIYRFHRAAGEGVSERPLIDGAEPHGIFATRHFNRPNRLGISVAR
                     LTGIDGRRIVVEGIDLLDETPILDIKPYIPAFDSIPDATSGWVTPGHVEAIERQSAAF
                     QAGRP"
     gene            complement(1544..2371)
                     /gene="modA"
                     /locus_tag="PLI76_06965"
     CDS             complement(1544..2371)
                     /gene="modA"
                     /locus_tag="PLI76_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOI61808.1"
                     /translation="MRKKIIVPAALIILAALLWTAGCTVQSPTTTDTNATPGASDVSL
                     FVYSGAGLKKPMNEIGKAFTEKTGINVEYNFAGAGALVSQMELSRKGDVFVSGGTPDY
                     RIARDKGLVGEPGYVAYHVPIIAFQKGNPKNITSVDDLARPGLKLALGGVESTAIGKA
                     GDILFEKHGIRDAVEENVVLRAPTINEVVIAMEMGTADAALLTLDSVNPETMDTIDLA
                     QEDSLTLIVPIGPTTFTTQPDAARQFVAFVTSDEGKAFFAKHGFPTYPDPRYAGIEP"
     gene            complement(2573..3055)
                     /gene="tsaA"
                     /locus_tag="PLI76_06970"
     CDS             complement(2573..3055)
                     /gene="tsaA"
                     /locus_tag="PLI76_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HOI61809.1"
                     /translation="MTGIAFTPIGTVRSPHHDPRDMPIQPVGARGIRGTIELDPTYAA
                     GLRDLEGFSRIILLYHFHRSEGYALEVVPFLDKTPRGVFATRAPRRPNAVGLSMLKLI
                     AVDGATLTVEDVDILDGTPVLDIKPYVPAFDAFPDERAGWLTATSGAARTMRSDGRFC
                     "
     gene            complement(3144..3779)
                     /gene="thiE"
                     /locus_tag="PLI76_06975"
     CDS             complement(3144..3779)
                     /gene="thiE"
                     /locus_tag="PLI76_06975"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOI61810.1"
                     /translation="MGYDLYVITDEEIGRGRSHAELARRAVAGGADVIQLRDKRLSGR
                     DLFRAAVAIRGITADAGALFIVNDRLDVALAAGADGVHLGENDLPVGEARRIAPPGFV
                     IGASVSSVATAVRAAAEGADYVALSPTFATASKDDAGPGYGLTMLAEIRAAVSLPLVA
                     IGGITAANVAGVIAAGADGVAVISAVVGEDDVTAAARDLRARITAAKAGRR"
     gene            complement(3780..4619)
                     /gene="thiM"
                     /locus_tag="PLI76_06980"
     CDS             complement(3780..4619)
                     /gene="thiM"
                     /locus_tag="PLI76_06980"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HOI61811.1"
                     /translation="MPGSTNHGSSARFMDGTIPAALLAAVREQRPLIHHITNSVTVND
                     CANITICAGAAPVMAEAPEEVADMVAAAGALVLNIGTLSAAQVEAMLAAGRRANDLGI
                     PVILDPVGAGATAFRTGTVQRLLNALEIAVLKGNAGEIGVLAGTGGSVRGVDSGGVAG
                     DPIETARECARLTGTIVSMTGEVDVVTDTRRVFLIRNGVPAMDRLSGTGCMAASVTAA
                     FVAVADDYAVAAAAALAAFGRAGERAAVSARGPYSFRTALFDELAGLSPEDLACHARI
                     EER"
     gene            4795..5748
                     /gene="mch"
                     /locus_tag="PLI76_06985"
     CDS             4795..5748
                     /gene="mch"
                     /locus_tag="PLI76_06985"
                     /EC_number="3.5.4.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018759 - methenyltetrahydromethanopterin
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HOI61812.1"
                     /translation="MRTTLSINETAMPVVREMIGYRDELGIAVAEQENGATCIDCGVH
                     VPGSYRAGALFVEACLAGLATAAITMGRVGSVPVPFLHITVSHPALACLGSQKAGWVL
                     KAGGYSAMASGPARALAAKPKDTYKTIGYRDTSVFGILALEADTLPDEEVTGFIAEKC
                     GIDPGDLYVLVAPTASLVGSIQISGRVVTATLHKLEAKGYDVCRIRHAAGRSPIAPVK
                     RTGIAAMGATNDCNIYCGSVSLVVEGYDPVFASLPSRTSPDYGRPFSRVLEDAGYDFL
                     KVDSLLAFSPAEVTINDGTSGEVYHFGSLNPDVLLESFEIR"
     gene            complement(5776..6048)
                     /locus_tag="PLI76_06990"
     CDS             complement(5776..6048)
                     /locus_tag="PLI76_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOI61813.1"
                     /translation="MSEDRKKVPDRFPWIIPFSCEGCGDCVEACPTGSIELVDLGRKI
                     DKAWITNPETCIGCGKCAQACVVGAVQMTSYVEKAILRYREKTTPE"
     gene            complement(6055..6549)
                     /locus_tag="PLI76_06995"
     CDS             complement(6055..6549)
                     /locus_tag="PLI76_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61814.1"
                     /translation="MEVIRMTVNLTERMKAWVEAMGCHLCVATPEGVPFVVLGRYARA
                     TADGEVAFALAEDEAGVIAPVLSRNPRVAFGVSRQGGIRAAYQFKGTGELLRSGDIFD
                     AVRARAKEEKGIDAGAVLLVRLTELYCTKPGYEAGLRLDGMAPEELDAWERQRWKDVP
                     RKRN"
     gene            complement(6551..7036)
                     /locus_tag="PLI76_07000"
     CDS             complement(6551..7036)
                     /locus_tag="PLI76_07000"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOI61815.1"
                     /translation="MVKFTKEMKEALRVPGKGGSLIYLCTSSRGGNPNIAAMRFVATH
                     MDDKILIADMFLLKTKANIKENPNVAVTICHPLDEGRWWVFKGKAVDMEYGFPRDFDW
                     YGANATDILNEWGNWNKAEPPDEVPPDIVFPKAVQRGVVVVHVEEVYSIEPGHVGEKI
                     V"
     gene            complement(7053..8141)
                     /locus_tag="PLI76_07005"
     CDS             complement(7053..8141)
                     /locus_tag="PLI76_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOI61816.1"
                     /translation="MAFDLSKCDFSRCKGECLVRCPYVSYGEDEAKRAVRSLIAGEPH
                     PILKDCITCAACNDFCPRGANPWDLIAERQEETGVLGIPADAKPSSDWLTKPATVIRR
                     GKPGGPLISAGGIYEVVPQAEFLTGQVFSDATIIGGGPYACGFTETHLGRASRPVKFL
                     PQFIENLARTAEEFGVDEIVFTHDACYNVATTLAMQQGVRVPFRPVHILEYIRNWLRD
                     HPDRIVNPLDIRIAVQGGCTTRYAPKGGDREIWSDWLADIFDMIGVTSVEEKRVYTGV
                     DRLCCGCGIFHTQHERAVDIQRKNVQDAADAGAEQLLFICPACISVMRATCRKMKLEP
                     IYITQLVKRALGEELGPAGTAAFGYPVK"
     assembly_gap    8342..8441
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8507..8698
                     /locus_tag="PLI76_07010"
     CDS             8507..8698
                     /locus_tag="PLI76_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61817.1"
                     /translation="MKKFRIELTGFLEMDTDQERDARVLAERILLEIRSVVAGHGGVQ
                     DYDIDIGSVTEKPLTKRYR"
     gene            complement(8712..10604)
                     /locus_tag="PLI76_07015"
     CDS             complement(8712..10604)
                     /locus_tag="PLI76_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOI61818.1"
                     /translation="MYRCFDKLDDAVIILDRDNTVARLNRSFQEIFGIDDETVRSMET
                     EEFTTSHLARFLPKGEGADRVIEALRYRQEVSHAACRIETPDGEVRWFSFSCRMMTEE
                     PYTGMALVRLRDITSGYSEAAWNASEVEAEVGAETIFAGIGELVPYGIWICDPDGTVL
                     YISPSFLDLIGTTLEECRQSGWLGRTPLGDPAGVLADWGRCLDAGCRWDRELRVPGPG
                     GEYRTILSRGGPIRDEDGRIILWAGINLDITARREAEQITAVRAAQQAVIADLGRLAL
                     AGTDLSELMNALVRAVAGHLGVKYAKVLRYLPDEDAFLLEAAVGFNETRVGKEKVEGG
                     TDSQAGYTLLSHEPVIVEDLRTETRFTGPALLRDEGIVSGISVIIQGKRAPYGVLGAH
                     TRTHRRFTQDDINFIQAAANILAQAIERRATEEALLASEEKFRAIAQRSFDMIYTCYH
                     DSGIAYISPAVTRMLGYTPEELIGRKCRDYLSPVSLPAWEEAEKKLARGEPVEGLEVE
                     FHRKDGFVAFVELNESAILEHGKVVGVQAVGRDITERKQNEQLRRQAFSQIERNIEQF
                     AVLGDHIRQPLQVTLGRAELIDDTETAAVIREQVERINDYIRQLDRGWVESRKVREFL
                     RRHDLE"
BASE COUNT         1845 a         3432 c         3468 g         1980 t
ORIGIN      
        1 agtcctggta gaccatgccg atcccccggt ctttcgggtc ggtgcgggtg atctcccggc
       61 cgtcgaggct gatcgtcccc gaagcgggcg catagatccc cgcgatcgtc tcgaggagga
      121 tggtctttcc cgcaccggtc ggtccgagta tgatgaggta ttcgccggca ccgacggtca
      181 ggtccacgcc gtcgagggag aactcgccga gggagaggga gagagccctg acgtcgagca
      241 tcagaaaacc ccccggccgt cggtgtagcg ctcaaatatc gcaagcgacg tcaccgatat
      301 gacgatcagg atgaccgacg cagcgatggc gagatcgagg tctcccgtcg acatattcag
      361 gaagagcgcg atgggcaggg tctcggtctt catctgggtg gccccggcaa gcatcaggac
      421 cgcgccgaac tcgccgatgg acttcgacca ggtgatgacg agaccggcga tcagaccggc
      481 ccgggccagg gggagggtga cctggcaaaa cgtcccccac tccgtgcacc cgagcgtcct
      541 ggcgacgtgc tcgtagcgag ggttgatcgt ctcgaaggcc gaccgggtga ccctgaccat
      601 gtagggaagg ttgacgaaga actgggcgat gatgatgccg agcggcgtat agatgatatc
      661 gaaccccgca gccgtgagca tctgcccgaa ctttgaaggg ccgtagaaga tgaggagcgc
      721 tacgcctgca acgaggggcg gaagggcgag cggtatgttg aagacggcgt ttacggcttt
      781 tttcccggga aacgagaacc gggcaagaga gtaggcgacc ggcacggcga cgacgacgca
      841 gagggcggtc gagatcgccg acgtgatgag cgagagcctg atggcaaaga ggatctcggg
      901 ggaggtgagg ctctcgatga gggccgggag cggcgggtag agtacaaggc cgacgatgac
      961 gacgaccatg taggtgacca ggccgaacag gaccgcgagg aagagagcct taaacgggga
     1021 gggatggggg agagcactcc ggtcccggat cacggtcgac cagcctggaa cgctgcgctc
     1081 tgccgctcga tcgcttcgac gtgccccggc gtcacccacc ccgatgttgc gtcggggatg
     1141 ctgtcgaatg ccgggatgta gggtttgatg tcgaggatag gcgtctcgtc caggagatcg
     1201 atgccttcga cgacgatccg ccttccatcg atcccggtaa gcctggcgac cgatatgccg
     1261 agccggttcg ggcggttgaa gtgccgggtc gcgaatatcc cgtgcggctc cgccccgtcg
     1321 atgagcggcc gctcggaaac cccttctccg gcggcgcggt ggaaccggta gatgaggatg
     1381 atgtgggaga accctgcaag cgcgatgagc ccgtcttcga accccgggta gacctccacc
     1441 gtcccgcggg cggtggagaa tacgctctgg atgggggttt cggccgggtc cgagaacggc
     1501 gtgcgggcaa tcccgatggc cttgcagaaa taatctgcac cgttcatggc tcgatccctg
     1561 cgtacctcgg gtccgggtag gtcgggaacc cgtgctttgc aaagaaggcc ttcccctcat
     1621 cggaggtgac gaacgcgacg aactgccttg cagcgtccgg ttgcgtcgtg aaggtggtcg
     1681 ggcctatcgg gacgatgagg gtgaggctgt cttcctgcgc cagatcgatg gtatccatcg
     1741 tctcggggtt gacgctgtcg agcgtgagga gggcggcatc ggcggtgccc atctccatcg
     1801 cgatcaccac ttcattgatc gtcggtgccc tgaggacgac gttttcctcg acagcatcgc
     1861 ggataccatg tttttcgaag agtatatcgc cggctttgcc gatcgcggtc gattccacgc
     1921 caccgagggc aagtttcagc cccggccggg cgagatcatc aacggaagtg atattcttcg
     1981 ggttcccctt ctggaaggcg atgatcggga cgtggtaggc gacgtacccc ggctctccga
     2041 ccaggccctt atcccgggcg atacggtagt cgggtgtccc gccggagacg aagacgtcgc
     2101 ccttccggga gagctccatc tggctgacga gtgcccccgc accggcgaag ttatattcga
     2161 cgttgatgcc ggtcttctcg gtgaacgcct tgccgatctc gttcatcggt ttcttgagac
     2221 ccgcgcctga gtagacgaag agcgatacat cgctcgcacc gggggtagcg ttggtgtcgg
     2281 tggtggtcgg tgattggacg gtgcagcctg ccgtccagag gagggcggcg aggatgatga
     2341 gtgcagccgg cacgatgatc ttctttctca tggtactgaa cggtacccac ctgccggtat
     2401 taaagaacta ttatttattt tgaggttatg ttaaactgtt ttatctgaca tactccctgt
     2461 tgaaggttgc tatcctcccc tacccggttg ccgtaccccc ggtatctgca gcggtcgcga
     2521 tggggcggta cagggtgtcc tttgcggtcg acccggggcc ccgccgggac cgtcagcaga
     2581 accggccgtc cgatcgcatg gtgcgggccg cccccgatgt cgcggtcagc cacccggcgc
     2641 gctcgtcggg aaacgcgtcg aaggccggga cgtagggttt gatatcgagg accggggtgc
     2701 cgtccagtat atccacgtcc tcgacggtga gcgtcgcgcc gtcgacggcg atcaacttga
     2761 gcatcgatag accgactgcg ttcgggcggc ggggtgctct ggttgcaaag acgccgcgag
     2821 gcgtcttgtc gaggaacgga accacctcaa gcgcgtagcc ctccgatcgg tggaagtggt
     2881 agaggaggat gatccgcgag aacccttcga ggtctctcag tccggcggca taggtcgggt
     2941 caagctcgat ggtaccccgg atgccccggg cgccgacggg ctggataggc atgtcgcgtg
     3001 ggtcgtggtg gggggaacgg acggtaccga taggggtgaa tgcgatgccg gtcatggata
     3061 ttctcctgag ggcgttatgc ggcggaagtg aagagggttt gtgtccgggc aggatcctgc
     3121 cttcaggaac ccgggagccg gattcagcgg cgccccgcct tcgcggcggt gatccgggcc
     3181 cggaggtccc gtgccgcggc ggtgacgtca tcctctccca cgaccgccga tatcaccgcg
     3241 acgccatccg cccccgcggc gatgacgccg gcgacgtttg ctgcggtgat cccgccgatc
     3301 gcgacgaggg ggagggagac cgcagctcga atctccgcga gcatcgtaag cccgtatccc
     3361 ggacccgcgt cgtccttcga tgcggtcgcg aacgtcgggc tgagcgccac gtagtcggct
     3421 ccctccgccg cggcccggac cgccgtcgcg acggagctca ccgaggcacc gatgacgaac
     3481 cccggcgggg cgatccgccg ggcctccccg acggggaggt cgttctcgcc gaggtggacc
     3541 ccgtcggcgc cggccgcgag ggctacgtcc aggcggtcgt tcacgatgaa gagcgcgccg
     3601 gcatcagcgg tgatcccgcg gatggcgacg gctgcccgga agaggtcgcg accggagagc
     3661 ctcttatccc ggagctggat aacgtccgct cccccggcaa cggcccggcg ggcaagctcc
     3721 gcgtgggagc gcccccggcc gatctcctca tcggtgatca cgtagaggtc gtatcccatt
     3781 tatcgctcct cgatccgcgc gtggcaggcg aggtcctcgg gggagagccc ggcaagttcg
     3841 tcaaagagcg ccgtccggaa cgagtagggc ccgcgggcag agactgccgc ccgctccccg
     3901 gccctcccga aggctgcaag cgccgcggct gccgcgaccg cgtaatcatc cgcgacagcg
     3961 acgaacgccg cggtgaccga tgcggccatg caccccgtcc cggagaggcg gtccatcgcc
     4021 gggacaccgt tcctgatgag gaagacccgg cgggtatcgg tgacgacgtc gacttccccg
     4081 gtcatcgata cgatggtccc ggtcaggcgg gcacactccc gggccgtctc gatggggtcg
     4141 ccggcaactc cgccggagtc caccccccgg acgctcccgc cggtcccggc aaggacaccg
     4201 atctccccgg cgttcccctt caagacggcg atctcgaggg cattaaggag gcgctgcacc
     4261 gtcccggtcc ggaacgccgt cgcccccgca ccgaccgggt cgaggatgac cgggatgccg
     4321 aggtcgtttg cccgccggcc ggcggcgagc atcgcctcga cctgcgccgc cgagagcgtc
     4381 cctatgttca ggacgagggc gccggcggcg gcgaccatat cggcgacctc ctcgggcgcc
     4441 tcggccatca ccggggcggc tccggcgcag atcgttatgt ttgcgcagtc gttgaccgtg
     4501 acgctgttcg tgatgtggtg gatgagcggt cgctgctccc gcactgcggc aaggagggcg
     4561 gcggggatcg tgccgtccat gaagcgtgcc gatgaaccgt gattcgtgct acccggcata
     4621 gataactatc tgtttgagcg gttgcataaa accggtcgcc ggatctccgc ggacctcacc
     4681 cgtcgatgcg atccctgtac cgggccgcgc ggccggccgg agaatactct ctcgcatgta
     4741 tgccgggata ggaattgcta aagcgatctg caccccaccc ctccgcgaag agaggtgagg
     4801 acgacgctca gtatcaacga gacggcgatg cccgtcgtga gagagatgat cggctaccgg
     4861 gacgaactcg ggatcgcggt cgcggaacag gagaacggcg cgacgtgcat cgactgcggc
     4921 gtgcacgtgc cggggagcta ccgggcgggg gccctcttcg tcgaggcctg ccttgcgggg
     4981 cttgcgacgg cggcgataac catgggccgg gtagggagcg tgcccgtccc gtttctgcac
     5041 atcaccgtga gccaccccgc gcttgcatgc ctcggttcgc agaaggcggg gtgggtgctc
     5101 aaggccggag ggtattctgc gatggcctcg ggtccggcgc gggcgctcgc ggcaaagccg
     5161 aaagatacct acaagacgat cggctaccgc gatacatcgg tgttcggcat cctcgcgctc
     5221 gaggccgata ccctcccgga cgaggaggtc acggggttta tcgcggagaa gtgcggcatc
     5281 gaccccggag acctctatgt cctggtcgcc ccgaccgcga gcctggtcgg ttcgatccag
     5341 atatccgggc gggtcgtcac ggcgaccctc cacaaactcg aggcgaaggg ttacgacgtc
     5401 tgccggatcc gccacgcggc agggagatcc ccgatcgcgc ccgtgaagag gaccggcatc
     5461 gcggcgatgg gggcgaccaa cgactgcaac atctactgcg gttcggtctc cctcgtcgtc
     5521 gagggttacg acccggtctt tgcgagcctc ccctcgcgga cctcccccga ctacgggagg
     5581 ccgttctccc gcgtcctcga agatgccggc tacgacttcc tcaaggtgga ctcgctcctc
     5641 gcgttctcgc cggcggaggt gacgatcaac gacggcactt ccggcgaggt ctaccacttc
     5701 ggcagcctaa accccgatgt gctgcttgag tcgtttgaga tccggtgaga caaaaagggg
     5761 atgggatccc ccggctcact ccggggtcgt cttctcgcgg tagcgcagga tggccttctc
     5821 gacgtaggac gtcatctgca cggcgccgac gacgcaggcc tgtgcgcact tcccgcaccc
     5881 gatgcaggtc tccgggttcg tgatccaggc cttgtcgatc ttcctcccga ggtcgacgag
     5941 ctcgatcgag ccggtcgggc aggcctcgac acagtcgccg cacccctcgc acgagaaggg
     6001 gatgatccag gggaaccggt cgggcacctt ctttctgtct tcgctcatcg tgcctcagtt
     6061 cctcttccgc ggcacatcct tccagcgctg ccgctcccac gcatcgagtt cctccggcgc
     6121 catgccgtcg agccggagcc ccgcctcgta gccgggcttc gtgcagtaga gctcggtcag
     6181 gcgcacgagg agcaccgctc cggcgtcgat ccctttctct tcctttgccc gcgccctgac
     6241 cgcatcgaag atatctcccg accggaggag ctccccggtc cccttgaact ggtatgcggc
     6301 gcggatccct ccctgccgcg atacgccgaa cgcgacccgc gggtttcgtg agagcacggg
     6361 ggcgatcacc cctgcctcgt cctccgcaag cgcgaacgcc acctcaccat cggctgtcgc
     6421 ccgggcatag cgtcccagca ccacgaacgg cacgccctcc ggcgttgcga cgcagagatg
     6481 gcagcccatc gcctctaccc atgccttcat cctctctgta aggttcaccg tcatcctgat
     6541 cacctccata ctacacgatc ttctctccca cgtgcccggg ctcgatggag tagacctcct
     6601 cgacgtgcac gaccacgacc cctcgctgga cggccttcgg gaagacgata tcgggcggca
     6661 cctcgtccgg cggttcggcc ttgttccagt tcccccactc gttgaggata tccgtcgcgt
     6721 tcgcgccgta ccagtcgaaa tcgcgcggga acccgtactc catatccacg gctttgccct
     6781 tgaagaccca ccagcgcccc tcgtcgagcg ggtggcagat cgtgaccgcg acgttcgggt
     6841 tctccttgat gttcgccttc gtcttcaaaa ggaacatatc ggcgatcagg atcttgtcgt
     6901 ccatgtgcgt ggcaacgaac cgcatcgcgg cgatgttggg gttgccgccc ctgctcgacg
     6961 tgcagagata gatcaggctc cctcctttgc cgggaacccg cagggcttct ttcatctcct
     7021 ttgtaaactt caccatgctt ctcacctcac aattatttca ccggataccc gaacgcggcg
     7081 gtcccggcgg gcccgagctc ctcgcccagc gcacgtttta cgagctgggt gatatagatc
     7141 ggctcgagtt tcatcttccg gcaggtcgcg cgcatcacgg agatgcaggc cgggcagatg
     7201 aagaggagct gttcagcccc ggcgtcggca gcgtcctgga cgttcttgcg ctggatatcg
     7261 accgcccgtt cgtgctgggt gtgaaagata ccgcacccgc aacagagccg gtcaactccg
     7321 gtatacaccc gcttctcctc aacggacgta acgccgatca tgtcgaagat gtcggcaagc
     7381 cagtccgacc agatctcgcg gtccccgccc ttcggggcgt agcgcgtcgt gcaaccgccc
     7441 tggaccgcga tccggatgtc gagcgggttc acgatccggt cggggtgatc gcgcagccag
     7501 ttccggatgt actcgaggat atgcaccggc cgaaacggca cccgtacccc ctgctgcatc
     7561 gcaagggtcg tcgccacgtt gtagcaggca tcgtgggtaa agacgatctc gtcgaccccg
     7621 aactcttctg ccgttctcgc gaggttctcg atgaactgcg gaaggaactt caccggccgg
     7681 gacgcccggc caagatgggt ctcggtgaac ccacaggcat acggcccgcc acctatgatg
     7741 gtcgcgtccg aaaagacctg tcccgtgagg aactccgcct ggggcaccac ctcgtagata
     7801 ccgccggcag agatcaacgg gccgcccggc tttcctctcc ggatgaccgt cgccggtttc
     7861 gtcagccagt cgctcgacgg ttttgcatcc gccgggatgc cgagaacgcc cgtctcctcc
     7921 tgccgctctg cgatgaggtc ccaggggttt gcgccccgcg ggcagaagtc gttgcaggct
     7981 gcgcaggtga tgcagtcttt caggatcggg tggggttctc ctgcaatcag ggatcgtacg
     8041 gccctctttg cttcatcctc gccgtatgag acatacggac agcggacgag gcattctcct
     8101 ttgcagcgtg aaaagtcgca tttcgagaga tcaaatgcca tcactcagcc ctctatttac
     8161 cctgtgtccg tatccttatc taacgtaggt tcccggcaat gtgagatccc gggacgattt
     8221 gcgcgcccct cccggcagga aacgtttcgg cgagcggccg cggagggtat cggcgtgcgc
     8281 atatcgctat tctttttatg ttaacgctgt taacgaggat gtgcaggcgc cccacgcggg
     8341 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nccggatgag gccgaccgcc
     8461 tgcggataac cgggaagggg tcgcccgtga gaaagaggtg attggtgtga agaagtttcg
     8521 tatagagctg acggggttcc ttgagatgga taccgaccag gagcgcgatg cacgagtgct
     8581 ggcggagcgg atcctcctcg agatccgctc ggtcgttgcc gggcatggcg gggtgcagga
     8641 ctatgacatc gatataggtt cggttacgga aaaaccgctc acgaagcgct acaggtaacc
     8701 aaccgcagga ctcactcgag atcgtgccgc cggaggaact cgcgaacctt ccgtgactcc
     8761 acccatcccc ggtccagctg ccggatgtag tcgttgatcc gctcgacctg ctcgcggatg
     8821 actgctgccg tctccgtatc gtcgatcagc tccgccctcc ccagggtcac ctgcaggggc
     8881 tggcggatgt ggtcgccgag gacggcgaac tgctcgatat tccgctcgat ctgggagaac
     8941 gcctgccggc gaagctgttc gttctgtttc cgttcggtga tatctcttcc cactgcctgg
     9001 acgccgacca ccttcccgtg ctcgaggatc gccgactcgt tcagctcgac gaacgccacg
     9061 aacccatctt tccggtggaa ctcgacctcg agcccctcga ccggttcgcc ccgggccagc
     9121 ttcttctctg cctcctccca cgcggggagc gatacggggc tgaggtaatc gcggcatttg
     9181 cgaccgatca gctcctcggg cgtgtaaccg agcatgcggg tcaccgccgg ggatatgtag
     9241 gcgatcccgc tgtcgtggta gcaggtgtag atcatatcga agctccgttg cgctatcgcg
     9301 cggaacttct cttcgcttgc gaggagcgcc tcctcggttg cccgccgctc gatcgcctgc
     9361 gcgagaatgt ttgccgccgc ctgtatgaag ttgatgtcat cctgcgtgaa cctgcggtgc
     9421 gtccgggtgt gggcgccgag caccccatag ggtgcccttt ttccctggat gatgacgctg
     9481 atcccgctca cgatcccctc gtcccggagg agggccgggc cggtaaaacg tgtctcggtc
     9541 ctgagatcct cgacgataac cggttcatgg gagaggagcg tgtagcctgc ctgggagtcg
     9601 gttccgccct ccaccttctc cttgccgacc cgggtctcgt taaaccccac cgccgcctca
     9661 agcaggaatg cgtcctcgtc ggggagatac cggagcacct tggcatactt gacgccgagg
     9721 tgcccggcca ccgcccggac cagggcgttc atcaactccg ataggtcggt ccccgcaaga
     9781 gcgaggcggc cgaggtctgc gatgaccgcc tgttgcgccg cccggacagc ggtgatctgt
     9841 tcggcctctc ttcttgcggt gatgtcgaga ttgatgccgg cccagaggat gatccggcca
     9901 tcttcatccc ggatggggcc accgcgcgag aggatggtgc gatactcccc gcctggaccc
     9961 ggaaccctga gttcacggtc ccaccggcac ccggcatcaa ggcagcgccc ccagtcggcg
    10021 agcaccccgg ccggatcccc aagcggtgtg cggcccagcc acccggactg ccggcactcc
    10081 tcaagcgtcg tcccgataag gtcgaggaac gaaggggaga tgtagagcac ggtgccgtcc
    10141 gggtcgcata tccatatgcc gtaggggacg agctcaccga tcccggcgaa tatcgtctcc
    10201 gctccgacct ctgcctccac ttccgaagcg ttccatgccg cctcactata cccggacgtg
    10261 atatcccgga gccggacgag ggccatcccg gtgtagggct cctccgtcat catacggcac
    10321 gagaatgaga accagcgcac ctcgccgtcg ggggtctcta tcctgcaggc ggcgtgcgag
    10381 acctcctgcc ggtaccggag ggcttcgatc acccggtcgg cgccctctcc cttcgggagg
    10441 aatcgcgcaa ggtggctggt tgtaaactct tccgtctcca tgctgcggac cgtctcgtcg
    10501 tcgatgccga agatctcctg gaacgatcgg ttcaaccgcg caacggtgtt atcccgatcc
    10561 aggataatga cagcatcgtc cagtttgtca aagcagcggt acacgggtga tccccatcgg
    10621 tcgataccaa tgccggtact gatgtaccgg gtttatgaat attactttct ggagcgctcg
    10681 cgatctgctg tggtaccagg atcacaggca gtggttcttg aggatggaaa ccggatgaga
    10741 gggatcgcgc acccccggtc gttgtagaga gacgccgagg gggagatttg aactcccgag
    10801 gtgccaggca ccagtggctt tcgag
//
LOCUS       DAOSEE010000077        10570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_2390_length_10570_cov_263.403043, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000077 DAOSEE010000000
VERSION     DAOSEE010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 10570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10570
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2390_length_10570_cov_263.403043"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1518)
                     /gene="purF"
                     /locus_tag="PLI76_07020"
     CDS             complement(85..1518)
                     /gene="purF"
                     /locus_tag="PLI76_07020"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOI61819.1"
                     /translation="MCGIVGIVDAGGVSFPLYYSLYALQHRGQESAGISSFEGTTLYT
                     HRVQGLVAEAFNSNTLQGLRGNVGIGHVRYPTAGSKVPENIQPFNFQYRGLNLAIAHD
                     GNLVNTAELREEYERRGQIFCTTTGTEVIGNIIVEALRTSRSMEDAVRLCMRRLRGSY
                     AVVALLNGTIYAFRDPLGIKPLCIGKIDRGYIVASESVAIDALDGTLVRDVRPGELVR
                     IDEDGLSSIQVATANRRAHCIFEYIYFARADSVIDGRLVYDVRRRIGQNLYDADPVEG
                     DIVCPVPDSGTAYAAGYAERSGIPFVEGLMKNRYMGRTFIMPTQQQREWAVRIKLNTV
                     RGNLKDRRVILVDDSIVRGTTSRRIVNMIREAGAKEIHLCVGSPPIIAPCYLGVDMPT
                     RTELIANSRDVEAVRESIGATSLTYVPFGDLMGAIGCGERDLCTGCLTGCYPVEILGE
                     KSCHCAVDYVAGTHQSNLSTFGKQQTS"
     gene            complement(1528..1710)
                     /locus_tag="PLI76_07025"
     CDS             complement(1528..1710)
                     /locus_tag="PLI76_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37e"
                     /protein_id="HOI61820.1"
                     /translation="MSKGTPSMGKRQKRTHIACRRCGKISFHAQHKVCAACGFGRSSR
                     IRSYRWTEKKAKVPTH"
     gene            complement(1725..1952)
                     /locus_tag="PLI76_07030"
     CDS             complement(1725..1952)
                     /locus_tag="PLI76_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOI61821.1"
                     /translation="MTPRPLDILDQVLNRQPVIISLKGGREIRGILQGYDVHMNLVLD
                     RAEEETGGTAQKLGTLIVRGDNVIYITPSIE"
     gene            2062..2559
                     /locus_tag="PLI76_07035"
     CDS             2062..2559
                     /locus_tag="PLI76_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOI61822.1"
                     /translation="MPKITVKKRHVIRKTQIAGLLDRLRAEIGVSAELFQSDRIERVE
                     TDAPVEIYLVDRKPLLMASGDWAFPTLRGLIEHPIPERRVTVDAGAVRFVANGADIMR
                     PGVVSISSDIRQGHPLQVVEERYGKPLAVGVALFDAADMERQEKGKVVKSLHFVGDDL
                     WNLEI"
     gene            2610..2987
                     /gene="sepF"
                     /locus_tag="PLI76_07040"
     CDS             2610..2987
                     /gene="sepF"
                     /locus_tag="PLI76_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HOI61823.1"
                     /translation="MVKKLFDSILGKSPVRDQDEYMELDLASYESVTEEEPASMYIKI
                     ATIADLKDTPRVKDEVYNGNIVIVDIGRLKMDKVTFERVLKDLRDVAKDVNGDIVGLG
                     EQKYVVITPMSVKVSREKIGGGL"
     gene            2987..3514
                     /locus_tag="PLI76_07045"
     CDS             2987..3514
                     /locus_tag="PLI76_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZPR1 zinc finger domain-containing protein"
                     /protein_id="HOI61824.1"
                     /translation="MREACPGTCPACGADIQIVHHRIDIPHFPDLLLVTIACDACGYR
                     HTDTIIPGEREPARWMVRIEEPGDLSTRVVRSTTGTIRIPELGLAVEPGTACEGFVTN
                     VEGVLSRFERAVAIILANPESDEEQEAALRMQEALAAAREVASPFTVILEDPAGNSAL
                     VGEKAEKVLLEEREA"
     gene            3589..3825
                     /locus_tag="PLI76_07050"
     CDS             3589..3825
                     /locus_tag="PLI76_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61825.1"
                     /translation="MARTISVLKWENKQDVDNAVHDIFAEMSERGGLTKDTERAMQHS
                     LLVADPDLTNYFLRTVMEQVPDAMHYFEERGGGA"
     gene            complement(3892..4884)
                     /gene="purM"
                     /locus_tag="PLI76_07055"
     CDS             complement(3892..4884)
                     /gene="purM"
                     /locus_tag="PLI76_07055"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOI61826.1"
                     /translation="MTEHTYREAGVDIDLEARAVRALIDTLTYRRTGAFPMLGKVGHF
                     AGLIDFGPYVLALAVDGVGTKMLVADALCDWRTVGIDCIAMNVNDLYVMNLEPVAFVD
                     YIATDALSVEKMAQIGQGLNEGARQANMNIVGGETATLRGLVNGLDLAGTCLGVQEKE
                     KVVTGESIAPGDVIVGVPSSGIHSNGLTLARRVVEEYASYDTLLANGRTLGEELLTPT
                     RIYAEVLRVTEACTVHGMCHITGGGLLNFSRLSGYGFSITDPLEVPAIFRWLQETGGI
                     GDVEMYRTFNMGMGYAFVVPAASVPVIRSIVPDARVVGKVTEEPGVRFKGIEMR"
     gene            complement(4877..6265)
                     /locus_tag="PLI76_07060"
     CDS             complement(4877..6265)
                     /locus_tag="PLI76_07060"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOI61827.1"
                     /translation="MKFGGTSVGEADCIRRVADIVEPHHRGGDEVALVVSACSGITDQ
                     IIAIADEVVKSMEQPPIGAFLAAMRTRHTRLLGEVAPDYADEVTAVIDDRLTRLQNIL
                     TAVHTLKELTPRSRDYIISFGERLSAPIVAAALRQRGIPSVALDGAEAGIVTTANHGD
                     ARALPSSETGIRSRVAPLLAGSVPVIMGFMGATEQGVVTTLGRSGSDYSAAVIGAGIG
                     ADEIWIWTDVDGVMTSDPRIIRDARVLNDISYLEVMELSYFGAKVLHPRSIEPAMQKD
                     IPIRVRNTFRPDYPGTIVLRDEHREKRVVKAIALIEKVALVNVNGAQMIGRPGVAKTI
                     FSALAEREVNVMMISQGSSEANISLIVDESHLEAAIAALTPIAKQGVVREVTCDRDVA
                     ALAVVGAGMAGTPGTGGRIFSALGRAQINTMMISQGSSEVNVSFVVKGGDGKRALQVL
                     HDEFRLSENSDD"
     gene            6338..7969
                     /locus_tag="PLI76_07065"
     CDS             6338..7969
                     /locus_tag="PLI76_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit A"
                     /protein_id="HOI61828.1"
                     /translation="MLVDEIVDAHVLVIGSGGAGARAAIEASRYGDVVLVSKTIAGKG
                     GCTTMAEGGFNAVLRDQDSTGVHREDTLKGGAYLNDPALVDLLTREAPERLADLVRWG
                     AVFDVTDDRQVAQRPFGGQRFPRTCYAGDRTGHEMMMTLLDRLDATDTQLYQEVSVVD
                     LVTGEKGAVAGAVALDRDGDPILFSADATVLATGGGTQAYDISTNSAAGTGDGFAMAY
                     RAGAELIDMEMVQFHPTGAVYPYDARGRLVTEAVRGEGGILLNARRERFMQRYDPDRM
                     ELSTRDVVARAIATEVLEGRGTSRGGVYLDVTHLPAERIETRLPVMLEQFLAYGVDIR
                     REPMEVAPTAHHIMGGLRITPECRTTIAGLFACGEVTGGVHGANRLGGNALADTQVFG
                     KRAGEFAGKTQEQSGRVDPARVDGVLRMLDAFFEGAVSPADVKKDLKLTMWNQAGIFR
                     SAPDLRTALGHIRRLADRRLFAASAANLLDCCTVRNMCTTASLIVRCALLRPENRGAH
                     VRRDAEVATDPATSPFGHTYVSLAREGIERREVPV"
     gene            7966..9429
                     /locus_tag="PLI76_07070"
     CDS             7966..9429
                     /locus_tag="PLI76_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HOI61829.1"
                     /translation="MKAMTIRVSRYDPAHDAEPHFEEYTIRVNEGARVLHALHAIRDE
                     HDPTLAYRYCCGSGQCGSCAVRVDGTPVLACMAEARDGMTVEPLDLPVLKDLTVEMSP
                     VIAKIARICPAPDAHLPAREEIEAIKPLRDCIECLCCVSACPALQVTEFAGPTVLRQE
                     MRLALDPRDSGDRIGDAIEKGLFYCTTCRRCLEVCPKEINIPGKAIEKLRELANRRGL
                     TLPRHREVAQLVQETGRSVERTKATFLEQVPEVIEPDGPVRGEVGFFVGCMFNGRVPQ
                     TALDAMEVMKRNGIRVIVPHDQVCCGSPLIRTGQTAFIDDLRQKNIEAFARRGIATVM
                     TICAGCGATLKNDYETPFAVKDVTEILTEYGIELPSKLDIRATYHDPCHLLRGQGISK
                     EPRALLRESVREFVEMPSQCCGAGGGVRSGVPEEAKALGAKRNDAVQATGADVVVTVC
                     PFCEFHIADCTDVPVKNLVTVLLEGYRKKDAEQKGPA"
     gene            complement(9508..9720)
                     /locus_tag="PLI76_07075"
     CDS             complement(9508..9720)
                     /locus_tag="PLI76_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HOI61830.1"
                     /translation="MIRQPVNSGSLRSVGYDLRPGILEIEFANGSVYQYLDTPATVYQ
                     ELLIAPSHRNYFARHIRDRYPYRKIG"
     gene            10012..>10570
                     /locus_tag="PLI76_07080"
     CDS             10012..>10570
                     /locus_tag="PLI76_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOI61831.1"
                     /translation="MHIPIAQPSLGNEESEAVLAVLASGMIAQGPVTAAFEEEFAAYC
                     GVPHAVAVSNGTTALHAALLAAGVGPGDEVIVPAFTFFATASAVSMCGAVPVFADVDA
                     ATATIEPADALTKVTEKTKAVIAVHLYGQPCDVPAVRKICDDEDLIFIEDAAQAHGAT
                     YRGKKAGNLGDLACFSFYATKNLATG"
BASE COUNT         1942 a         3313 c         3440 g         1875 t
ORIGIN      
        1 ccttcaccgg gatctctaac tatcagggcc aagtcgcaaa aagagagggg tgccgccggg
       61 gagcttcgcc ggcaacggcg cgggctatga cgtctgctgc ttcccgaagg tcgagaggtt
      121 ggactggtgc gtgccggcca cgtagtccac ggcgcagtgg cagctcttct cccccaggat
      181 ctccaccggg tagcacccgg tcaggcaccc ggtgcagagg tcgcgctcgc cgcacccgat
      241 cgcgcccatc agatccccaa acggcacgta ggtgagcgat gtcgccccga tgctctcacg
      301 aaccgcctcg acgtccctgc tgttcgcgat cagttccgtg cgggtgggca tatccacgcc
      361 gaggtagcag ggggcgatga tcggcggaga accgacacag aggtggatct cctttgcccc
      421 cgcctcacgg atcatgttca cgatccgccg cgacgtcgtc ccccggacga tgctgtcgtc
      481 gaccaggatc acccgccggt ctttcaagtt tccgcggacg gtgttgagct tgatccggac
      541 cgcccactcc cgctgctgct gggtcggcat gatgaacgtc cggcccatgt aacggttctt
      601 catcagcccc tcgacgaacg ggatgccgga ccgttcggcg tagccggcgg cgtaggcagt
      661 cccggagtcg gggaccgggc agacgatatc gccctcgacg gggtctgcat catagagatt
      721 ctgcccgatc ctgcgccgca catcgtagac cagtctcccg tcgatgaccg agtcggcgcg
      781 ggcaaagtag atgtactcga atatgcagtg cgcccgccgg tttgcagtcg caacctggat
      841 cgaggagagc ccatcttcat cgatccggac gagctccccc ggacgcacgt cccgaacgag
      901 cgtgccgtcc agcgcatcga tcgccacgct ctccgaggcg acgatgtagc cccggtcgat
      961 ctttccgata cagagcggtt tgatgccgag tgggtcgcgg aaggcgtaga tggtcccgtt
     1021 taacagtgcg acgacggcat aggagcctct caggcgccgc atgcagagcc ggacggcatc
     1081 ctccatgctc ctggatgtcc ggagcgcttc gacgatgatg ttccctatca cctcggtccc
     1141 ggtcgtcgtg cagaagatct gtccccggcg ctcgtactcc tcccggagtt cggcggtgtt
     1201 gaccaggttg ccgtcgtggg cgatcgcgag gttgagcccc cggtactgga agttgaacgg
     1261 ctggatgttc tccggcacct tcgacccggc cgtcgggtag cggacgtggc ctataccgac
     1321 gttgccccgc agcccctgca gggtgttgct gttgaaggcc tcggcaacga ggccctgcac
     1381 cctgtgcgtg tacagggtcg taccttcaaa agaggagatt cccgcgctct cctgcccccg
     1441 gtgctggaga gcatagaggg aatagtacag cggaaaagaa acaccgccag catcgacgat
     1501 gccaacgata ccacacatga tccagctcta gtgcgtcggt acctttgctt tcttctccgt
     1561 ccagcggtaa ctgcggatcc tgctgctcct gccaaacccg caggctgcac agaccttgtg
     1621 ctgtgcgtgg aacgagattt tgccgcaccg cctgcaggcg atatgggtac gtttctgccg
     1681 tttgcccatg gagggtgtgc cttttgacat gaactctcac ctactcattc aattgaaggg
     1741 gttatgtaaa ttacgttgtc gccgcggacg atcagcgtac cgagtttctg cgctgtgccg
     1801 ccggtctctt cttctgccct gtccaatacc agattcatgt gaacgtcgta tccctggagg
     1861 ataccccgga tctcccttcc acctttgagg ctgatgatga cgggctgacg attcagtacc
     1921 tgatctaaaa tatccaacgg tcttggcgtc atatgattac cctttgccgt cctttctgga
     1981 gtaatatatt tgagtgagga agctacttaa atataaccgc actgttgcgg ggagcatgtt
     2041 gcaccccagg gtaagagttc catgccgaag atcaccgtga aaaaacgcca cgtcatcaga
     2101 aaaacgcaga tagcagggct tctcgaccgg ctccgcgccg agattggagt ctcggccgaa
     2161 ctgttccaat ccgaccggat cgagcgggtg gagacggatg cccccgtcga gatctacctt
     2221 gtcgatagaa aaccgctcct tatggcctcc ggggattggg ccttccccac cctgcggggc
     2281 ctcatcgagc acccgatccc cgagcgccgg gtgacggtcg atgccggcgc ggtcaggttc
     2341 gttgcaaacg gcgcggacat catgcgcccc ggtgtcgtct cgatatcgtc ggatatacgt
     2401 caagggcacc cgctacaggt cgtcgaagaa cgttacggta aaccgctcgc tgtcggggtt
     2461 gctctcttcg atgccgccga tatggagcgg caggagaagg gcaaggtggt caaaagcctc
     2521 cactttgtcg gggatgatct ctggaatctt gagatctgac gcggaaatct agagatacta
     2581 ttaaataaaa ttctttcctc attttctcta tggttaaaaa gctctttgac agcatcctcg
     2641 gcaaaagccc cgtcagggat caggacgaat acatggagct cgacctcgcc tcctacgaga
     2701 gcgtaaccga agaagaaccg gcatccatgt acatcaagat cgccaccatc gctgacctca
     2761 aggatacccc ccgcgtcaaa gacgaggtct acaacggcaa catcgtcatc gtcgatatcg
     2821 ggcggctgaa gatggacaag gtgacgttcg agcgggtctt aaaggacctc cgcgacgtgg
     2881 caaaagacgt gaacggggat atcgtcggtc ttggtgagca gaagtacgtc gtcatcacgc
     2941 cgatgtccgt caaggtctcc cgcgagaaga tcggcggagg cctgtagtgc gggaggcctg
     3001 ccccgggacc tgcccggcgt gcggggccga tatccagatc gtacaccatc gcatcgatat
     3061 tccccacttc cccgacctcc tgctcgtcac gatcgcctgc gacgcctgcg gctaccggca
     3121 caccgacacc atcatcccgg gggaacgcga gcccgcccgg tggatggtgc gcatcgagga
     3181 gccgggcgac ctttccaccc gggtggtgcg gagcacgacg gggacgatcc ggatccccga
     3241 actcgggctt gcggtcgagc ccgggaccgc ctgcgagggg ttcgtcacga acgtcgaggg
     3301 ggtcctctcc cggttcgagc gggcggtggc gatcatcctc gcgaaccccg agagcgacga
     3361 ggaacaggag gccgcgctcc ggatgcagga ggcgctcgcc gccgcacgag aggtagcctc
     3421 cccgttcacg gtcatcctcg aggacccggc cgggaacagc gcgctcgtcg gagagaaggc
     3481 cgaaaaggtg ctcctcgaag agagagaggc ctgatacctc ttttttgccc catcccgggg
     3541 taagcctcaa atataaccac ccccatatgc agcgccgggt gaaaaagaat ggcacgaacc
     3601 atatcggtgc tgaagtggga gaacaagcag gatgtcgaca acgccgtcca cgacatattc
     3661 gcggagatga gcgagcgggg cgggctcacc aaggataccg agcgggcgat gcagcactcg
     3721 ctcctggtgg ccgatcccga cctcacgaac tacttcctgc ggacggtcat ggaacaggtc
     3781 cccgacgcga tgcactactt cgaggagcgc ggcggcgggg cgtgagcacc ccgggggagg
     3841 gatggtcccg gacagcgccg cacgaaagaa cgcgggggaa tacccccgca ttcaccgcat
     3901 ttcgatgccc ttgaaccgga ccccgggctc ctccgtcacc ttcccgacca cccgggcatc
     3961 ggggacgatc gatcggatca cgggcacgct tgccgccggc acgacgaagg catagcccat
     4021 gcccatgttg aaggtgcggt acatctccac gtcgccgatg ccgccggtct cctgcagcca
     4081 gcggaagatg gccgggacct cgagcgggtc ggttatggag aacccgtacc ccgagagcct
     4141 tgaaaagttc aggaggccgc cgccggtgat gtggcacatg ccgtggacgg tgcaggcctc
     4201 cgtcacccgg agcacttctg cgtatatccg ggtcggcgtc aggagttctt cgccgagggt
     4261 tctgccgttt gcaagaaggg tgtcgtaaga tgcgtactcc tcgacgaccc ggcgggcgag
     4321 cgtcagaccg ttgctgtgga tgccgcttga ggggacgccg acgatgacgt cgccgggggc
     4381 gatcgactcg ccggtgacga ccttctcctt ctcctggacc cctagacacg tccccgcaag
     4441 gtcgaggccg ttcacgagcc ccctgagcgt cgcggtctcg ccgccgacga tgttcatgtt
     4501 cgcctgccgg gcgccctcgt tcaggccctg cccgatctgg gccatcttct cgaccgagag
     4561 ggcgtcggtc gctatgtagt cgacgaacgc tacgggttcg aggttcatca cgtagaggtc
     4621 gttgacgttc attgcgatgc agtctatccc gaccgtccgc cagtcgcaaa gcgcgtcggc
     4681 aacgagcatc ttcgtgccga cgccgtcgac cgcaagagca aggacatacg gaccgaagtc
     4741 gatcagaccg gcaaagtgcc cgaccttccc gagcatcggg aatgcgccgg tcctccggta
     4801 ggtgagggta tcgatcagcg cccgcaccgc ccgggcctcg aggtcgatat cgacccccgc
     4861 ctcgcggtag gtatgttcag tcatcggagt tctccgaaag ccggaattcg tcgtgcagta
     4921 cctggagagc ccgtttgccg tcgccgccct tcacgacgaa cgagacgttc acctccgagg
     4981 atccttgcga tatcatcatg gtgttgatct gcgcccgccc gagcgccgag aagatccggc
     5041 cgcccgtccc gggggtgccg gccatccccg cgcccacgac ggcaagcgcg gcgacgtccc
     5101 ggtcgcaggt tacttcccgc acgacgccct gcttcgctat cggggtgagg gccgcgatag
     5161 cggcttcgag gtgggactca tcgacgatga gggatatgtt ggcctcgctt gagccctgcg
     5221 atatcatcat gacgttcacc tcccgctccg cgagcgcgga gaagatcgtc ttcgcaacgc
     5281 ccgggcggcc gatcatctgc gcaccgttga cgttgaccag ggccaccttc tctatcaggg
     5341 cgatggcctt gaccacccgc ttctcgcggt gttcgtcgcg gaggacgatc gtcccggggt
     5401 agtcgggccg gaatgtgttc cggacccgga tcggtatatc cttctgcatc gcgggttcga
     5461 tcgaccgcgg gtggaggacc ttcgcgccga agtaggagag ctccatcacc tcaaggtagg
     5521 agatgtcgtt gaggacccgg gcatctctga tgatacgggg gtcagatgtc atcaccccgt
     5581 cgacgtcggt ccagatccag atctcgtcgg caccgattcc ggcgccgatg acggctgccg
     5641 agtagtctga gccgctccgc ccgagggtgg tgacgacgcc ctgctcggtc gcccccataa
     5701 aacccatgat caccgggacg gagccggcaa gcagcggcgc gacccggctc cggatgcccg
     5761 tctcgctcga gggaagggcg cgggcatctc catggttcgc cgtcgtgacg ataccggcct
     5821 ctgcgccgtc gagggcgacc gacgggatac cccgctgccg gagggcggcc gcgacgatcg
     5881 gggcggagag cctctcgcca aaggagatga tgtagtcgcg ggaccgggga gtcagctcct
     5941 tcagggtgtg cacggcggtg agtatgttct gcagccgggt gagccggtcg tcgatgacgg
     6001 ccgtcacctc atcggcgtag tcgggagcca cctccccgag gagccgggtg tgccgggtcc
     6061 gcatcgcggc aaggaacgcc ccgatggggg gctgttccat gctttttacg acctcgtcgg
     6121 ctatagcgat gatctggtcg gtgatccccg agcacgccga tacgaccagg gcgacctcgt
     6181 cgcctcctct atggtgtggt tctacgatat ctgcaacccg ccggatacag tcggcttccc
     6241 cgacggatgt gccgccaaac ttcattaaaa gcctcattct ggctcacgtg tcccctgcaa
     6301 ttactttatg gtagtataca acgcacagac taataagatg cttgtagacg agatagtgga
     6361 cgcgcatgtg ctggttatcg gaagcggcgg cgccggcgcg cgggctgcga tcgaagcctc
     6421 gcgatacggc gacgtggtcc tggtctccaa gaccatcgcc ggaaaaggcg gatgtacgac
     6481 gatggcagaa ggcggattca acgccgtgtt gcgggatcag gactcgaccg gcgtccaccg
     6541 cgaggatacg ctcaaggggg gggcatacct caacgatccc gcgctcgtgg atctcctcac
     6601 ccgggaggcg ccggagcggc tcgcggatct cgtccggtgg ggggcggtat tcgacgtcac
     6661 ggatgaccgg caggttgcgc agaggccgtt tggggggcaa cggttccccc ggacctgcta
     6721 cgccggggac cggaccggcc acgagatgat gatgaccctc cttgatcgcc tcgacgcgac
     6781 ggatacgcag ctctaccagg aggtctcggt cgtcgacctg gtaacgggcg agaagggtgc
     6841 ggtcgcgggc gcggtcgctc tcgaccggga cggggatccc atcctcttct cggccgacgc
     6901 gacggtgctt gcgacggggg gcgggacgca ggcctacgac atatccacga actccgccgc
     6961 cgggaccggg gacgggtttg ccatggccta ccgcgcaggg gcggagctga tcgatatgga
     7021 gatggtccag ttccacccga cgggcgcggt ctacccctac gacgcccgcg gccggctggt
     7081 gacggaggcg gtccggggcg aaggggggat cctcctgaac gcgcgaaggg aacggttcat
     7141 gcagcgctac gatcccgacc ggatggagct ctcgacacgg gacgtggtgg cccgggcgat
     7201 agcgaccgag gtgctggaag ggcgcgggac gagccgcggc ggggtctacc tcgacgtgac
     7261 ccatctcccg gcggagcgga tcgagacccg gctcccggtg atgctcgagc agttcctcgc
     7321 ctacggcgtg gatatacggc gggagccgat ggaggtggcg cccaccgccc accacatcat
     7381 gggcgggctc cggatcaccc cggagtgccg gacgacgatc gccggcctct tcgcctgcgg
     7441 agaggtgacc ggcggggtgc acggcgcaaa ccgtctcggc gggaacgcgc ttgcggatac
     7501 gcaggtcttc gggaaacggg ccggcgagtt cgccgggaag acgcaggagc agagcggccg
     7561 ggtcgacccc gcccgggtcg acggggtgct ccggatgctt gacgccttct tcgagggggc
     7621 cgtgagcccc gcggatgtga agaaagacct gaaactgacg atgtggaacc aggccgggat
     7681 cttccggagc gctcccgacc tccggacggc tctcgggcat atcaggcggc ttgccgaccg
     7741 gcgtctcttc gccgcgtcgg ccgcaaacct ccttgactgc tgcacggtcc ggaacatgtg
     7801 caccacggca tcccttatcg tccggtgcgc cctgctccgg ccggagaacc ggggagccca
     7861 cgttcgccgg gacgccgagg tcgcgaccga tccggcgacg tcgccgtttg ggcacaccta
     7921 cgtctccctc gcccgcgagg ggatcgagcg gcgggaggtg ccggtatgaa ggcgatgact
     7981 attcgggtct cccgttacga cccggcccac gacgcggagc cgcacttcga ggagtatacg
     8041 atccgggtca acgaaggcgc ccgggtgctc cacgccctcc acgcgatccg cgatgagcac
     8101 gacccgacgc tcgcctaccg ctactgctgc gggtcggggc agtgcgggag ctgcgccgtc
     8161 agggtggacg ggacgcccgt ccttgcctgc atggcggagg cccgcgacgg catgacggtc
     8221 gagccgctgg atcttccggt cttgaaagac ctgacggtgg agatgagtcc ggtgatagca
     8281 aagattgccc ggatctgccc ggcaccggat gcacatctcc cggcacggga ggagatcgag
     8341 gcgatcaagc cgctccggga ctgtatcgag tgcctctgct gcgtctcggc ctgcccggcg
     8401 ctccaggtga cggagtttgc ggggccgacg gtgctccggc aggagatgcg cctcgccctc
     8461 gatccccggg actcggggga ccggataggc gatgcgatcg agaaggggct cttctactgc
     8521 acgacctgca ggcgctgcct tgaggtctgc ccgaaggaga tcaacatacc gggcaaggcc
     8581 atcgaaaagc tccgggaact tgcgaaccgg cgcgggttga ccctcccccg ccaccgggag
     8641 gtggcgcagc tggttcagga gacgggccgg agcgtcgagc ggacgaaagc caccttctta
     8701 gagcaggtgc cggaggtgat cgagcccgac ggccccgtcc gcggcgaggt ggggttcttc
     8761 gtcgggtgca tgttcaacgg ccgggtgccg cagacggccc tcgatgcgat ggaggtcatg
     8821 aagcgcaacg gtatccgggt gatcgtcccc cacgatcagg tctgctgcgg ttcgccgctg
     8881 atccggacgg ggcagacggc gttcatcgac gacctgaggc agaagaacat cgaggccttc
     8941 gcccggagag ggatcgcgac cgtgatgacg atctgcgccg ggtgcggggc aacgctgaag
     9001 aatgattacg agacgccgtt cgcggtgaaa gacgtcaccg agatcctgac ggagtacggg
     9061 atcgagctcc cgtcgaaact cgatattcgt gcgacctacc acgatccctg ccaccttctc
     9121 cggggccagg gtatcagcaa agagccacgg gcgctcctcc gcgagagcgt cagggagttc
     9181 gtggagatgc cctcgcagtg ctgcggggcc ggcggcggtg tccggtcggg cgtgccggag
     9241 gaggcaaaag ccctcggagc aaagcgcaac gatgccgtgc aggcgacggg cgccgatgtg
     9301 gtggtgacgg tctgcccgtt ctgcgagttc catattgcgg actgcacgga tgtgcccgtg
     9361 aagaacctgg tgacggtact cctcgagggc taccggaaga aggacgcgga gcagaagggc
     9421 ccggcctgaa ccagcaggag gggaacggcg cccctctggc tgattcccgc ccagacgtat
     9481 cgggccgggg gtctcgagtg aagcggttca cccgatcttt cgataggggt agcgatccct
     9541 tatgtgccgg gcgaagtagt tccggtggga gggggcgatc agcagctcct ggtagaccgt
     9601 tgcaggcgta tccaggtact ggtagaccga accgttcgca aactcgatct ccaggatgcc
     9661 gggtcgaaga tcgtatccga ccgaccggag gctgccggag ttgacgggtt ggcgtatcat
     9721 gagagggggc taatgtcatc agagtatata tagtatttca ttcgttcttt tcacgaaatt
     9781 cgcaatattt ccgccttgat cgtaattaca gggaaatatt ttatgtgttt gtagaatcgt
     9841 ccgaagatat ggacgacgga gtgccgggat ggccttgtaa ttcacgcaca ttccaaaacg
     9901 gagaatatag cgaccgtatt ccggatcaca tccggtgtct ggatctcaaa aggcaacccg
     9961 tatatgaaac cccacgaaag agacatgaca ccagcacccg gagagagatc catgcacatc
    10021 cctatcgccc agccatccct cgggaacgag gagagcgagg ctgttctcgc cgtccttgcc
    10081 tccggcatga tcgcccaggg gccggtcacc gccgcctttg aagaggagtt cgccgcctac
    10141 tgcggcgttc cccacgctgt tgccgtcagc aacggcacca ccgccctcca cgcggcgctg
    10201 ctcgccgccg gcgtcgggcc gggggacgag gtgatcgtcc cggccttcac gttctttgcg
    10261 accgcttcag cagtctcgat gtgcggggcg gtgccggtct ttgccgacgt cgatgccgcg
    10321 accgccacga tcgagcctgc cgatgccctc acgaaggtca ccgagaagac gaaggccgtg
    10381 atcgccgtcc acctctacgg ccaaccctgc gacgttccgg cggtcaggaa gatctgcgac
    10441 gatgaggacc tcatcttcat cgaagacgcc gcccaggccc acggggccac ctaccggggg
    10501 aagaaggccg ggaacctcgg cgacctcgcc tgcttctcct tctacgccac gaagaacctg
    10561 gcgaccgggg
//
LOCUS       DAOSEE010000078        10390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_2447_length_10390_cov_240.313014, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000078 DAOSEE010000000
VERSION     DAOSEE010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 10390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10390
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2447_length_10390_cov_240.313014"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1843)
                     /locus_tag="PLI76_07085"
     CDS             complement(<1..1843)
                     /locus_tag="PLI76_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61832.1"
                     /translation="MKQKITSAISSGDPRELEKVVLDISETQTRKEKKSLYEQMNAAL
                     VTAAFEGGQPELLSQLVEPTKEEIFDLVRRAAGLYSQNKDDIWFGAIFSLVNKLDRKS
                     HQSDILAEISRSLVQTGVDTGDIHYIEKGGEAFDKISIRKYRSAILSEIIPLLIQYGQ
                     KYQNIDIMRHALLMLSEIGDISRQSQLHADVARAIAGSGIESGNIALVISGLSSATEI
                     NQKIRRTNSIADIVDATWKSSQKKEIADIEQIIDALPDVPEERLTEVLAILTEQLLDR
                     QRDKKQVYARLIRIDDERPWAGQTLILELLKKAERSGERWYLEKAFEFNTRSTVEAKL
                     PIEEIVLSGIAVVEKSGNSTILLDIMPLIDESCDATKAAQLYRQITDALSRMGDFYHA
                     IEVMKKASTSAQRINAQFFDTSVRLIKEGIRRGEVDLVRENILATLDIDIAESLVHQA
                     VTDFCKEYDFDEVVRHIPAVKELVSSHRTQDHLLIECNTILIERGFIRERDPSALVDL
                     VSQIGEQALREQAISTISVEMARIGVARKDRDLLRHATGLTCEIVDQQTRAEAMGGIV
                     DQAAVLAVEDGDLELLHGMRVLSTALLEREYCLFAMGKVIHGLIMYGI"
     gene            complement(2079..3437)
                     /gene="glmM"
                     /locus_tag="PLI76_07090"
     CDS             complement(2079..3437)
                     /gene="glmM"
                     /locus_tag="PLI76_07090"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOI61833.1"
                     /translation="MQKKKQMFGTNGVRGVVGETMTPALVLKIGAALGSMRKGTIAVG
                     RDTRTSGEALAHALKAGLLMTGCDVVDMGILPTPALQYIIKTGRFSGGAMITASHNPP
                     EYNGVKVIEADGTEMSDEEIIRLEDRFFAGEFEAASWEGVGAETAAPDRLEEYIEAVV
                     RHFPAGIGEGMTVVVDPGSGPAALTTPIILSRMGCRVHTINARLDGTFPGRMPEPTPE
                     GLRPLSEMVVATGADFGVAHDGDADRAVFVDNKGCYIEENYEFGLIEDYVCARNGGGL
                     VVTPVATSRLIRDIAEKHGCTVDYTPVGSIYVARRMIELIGGGKKVSFGGEGNGGLIY
                     SDHQFCRDGGMTAAMMVAVLASHNGRRLSDIQGDLPLYHLVKEKHHTHDPAALVRAIE
                     EAFAGEAIEKIDGIKIVRDDAWALVRASGTEPMIRVMIESRDPDIADAMYREIVQVVG
                     RV"
     gene            complement(3442..4197)
                     /gene="mobB"
                     /locus_tag="PLI76_07095"
     CDS             complement(3442..4197)
                     /gene="mobB"
                     /locus_tag="PLI76_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HOI61834.1"
                     /translation="MKIIQIVGRSNSGKTTFIRNLIPALSARGTVGAIKHLGHHGFQL
                     EPEKDTTLFHESHAALSGGVDETKSVVVRRENDLDSTLDLLCNAGVEYAILEGFKSRL
                     FPRVVIGDLASENVVLRNPTVDEVIAALPKFEDHYTIEGLVRELKRECGVSRAGAILT
                     FNGIVREWTGTERTDHMDFDDTVDALVESLRREMEAVPGIIGARFYHRKGRLYAGEDI
                     TYIALLAEHREEAFAAAIRAIDRLKRELHDVEK"
     gene            complement(4194..4742)
                     /locus_tag="PLI76_07100"
     CDS             complement(4194..4742)
                     /locus_tag="PLI76_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5806 family protein"
                     /protein_id="HOI61835.1"
                     /translation="MEEPDNRKDPNKYRKFKKVDGATYQRVNQFLRKHTHITAREWAI
                     ARLCADFKTTSGSEMTFIGENLPDLCPFMVDSYTPQAVNQARSSFKKKVKKAGATFFY
                     GAMCGFFTAEELDEILFEASEVARFLLEVEGTSLNIDDEIDVEDRITGVMRGVAEAAS
                     VILKSRPGQEDEKENPTGEEEP"
     gene            5987..6904
                     /locus_tag="PLI76_07105"
     CDS             5987..6904
                     /locus_tag="PLI76_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOI61836.1"
                     /translation="MEKTPFSEWLDRFSNYLRMRNYSPRTIRKYEQTIERFARYAWFR
                     EHSGPGPFDETACGDAPLDADVNVSAALVTDFFSALSEERDYKPKTLHRMISTLSSFY
                     RYLYAQGAVVANPMPGVERPRVKNQELKYLKHSQVVRLLNTIPAGRDRLIVRLIYATG
                     VRVSELCAINVEDIDFEEQTIRVKGKGDKFRTVFVDEETLGEIERFVGNSIEGPLFLG
                     QQGNHISPRTVQHLFRQYAPPGITPHKIRHSYASELYRRSKNLRVVQENLGHSSIKTT
                     EIYLHTDIEERRSVYRQYFPLSNGKKIDE"
     gene            7273..8208
                     /locus_tag="PLI76_07110"
     CDS             7273..8208
                     /locus_tag="PLI76_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOI61837.1"
                     /translation="MRYIVTGGAGFIGSNLAEHLARDHDVVIIDNLSSGRRENIEHLI
                     GHPRVTFIEGSVTDLALLTGAFAGADGIFHQGAIASVPRSVEKPLETNAVNVGGTVNV
                     LWAAKECGVPAVVAASTSAIYGDDPVFPKHETMAPTPLSPYAVSKLAGEYYGKVFSDL
                     YGIRTVFLRYFNVFGPRQDPNSEYAAVIPKFITRLLAGKPPIIYGDGEQTRDFIFVAD
                     VVRANVQAMEGNATGVFNIAGGRRISLNELAARLSGIAGMHARPLYGPVRPGDVRDSL
                     ADISRARDAFGFSPRSTVDEGLRETVAWFRDRGSL"
     gene            8378..8749
                     /locus_tag="PLI76_07115"
     CDS             8378..8749
                     /locus_tag="PLI76_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOI61838.1"
                     /translation="MTATDLHDPAVLNNRGVALERQGRYEEALAAFSAALLCDDDDVY
                     AWNNRAVILTRLGRPQEALLACKEALAVDRSCIFAWVTYGTVLGRIGRYVEAESAFAV
                     AEDLDPQARALYPGNSTMTRA"
     gene            8942..10291
                     /locus_tag="PLI76_07120"
     CDS             8942..10291
                     /locus_tag="PLI76_07120"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012108020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HOI61839.1"
                     /translation="MKTLILAGGSGTRLFPLSREHYPKQFIPLFDNESLFQKTIKRAL
                     LFSSPQDIAIVTNKEHRFLVRDQLAAIGCNSKVIEEPVGRNTLPAIYYGVREVARGEP
                     ETIAVLPSDHLITATGSFLAAFRRAEHLTKDYLVVFGVRPTSPHTGYGYIRPGDSLGD
                     GSLVDAFVEKPDLATAGRYVADGYLWNSGMFCFDAGLFLAECETCAPDVVRAFERPAD
                     EAYHAVPSLSIDYGIMEKTRRAAVVPLECDWNDVGNFDALYAALPKNGDKNAVRGEHI
                     GIDSSENLIIADRLVATVGVRDLAIVETKDAILVAARNEAQRVGEIAKTLREKGDSRA
                     LFHTQVHRPWGSYTNLEEGRSYKIKRVTVPPKRRLSLQMHHHRSEHWVVVTGCAEVTI
                     ADKTYLLRNGESTFVPAGTVHRLANPGLLPLELIEVQIGEYTGEDDIVRFEDDFERA"
BASE COUNT         1788 a         3297 c         3166 g         2139 t
ORIGIN      
        1 cgatcccgta catgatcagg ccatggatga ccttgcccat cgcaaagagg cagtactccc
       61 gctccagcag ggccgtggag aggacccgca taccgtggag cagttcgagg tcgccgtcct
      121 ccacggcgag cacggccgcc tggtccacga tgccccccat cgcctccgcc cgtgtctgct
      181 ggtccacgat ctcgcaggtg agcccggtcg catgccggag gagatcccga tctttccggg
      241 cgacgccgat acgcgccatc tcgacggata tggtcgatat cgcctgttcg cggagagcct
      301 gctcgccgat ctggctcacc aggtccacga gggccgacgg atctctctcc cggatgaacc
      361 cccgctcgat caggatggtg ttgcactcga tgagcaggtg gtcctgtgtc ctgtgcgagc
      421 tcacgagttc ctttacggcc ggtatgtgcc tgacgacctc atcgaagtca tactccttgc
      481 agaagtcggt caccgcctgg tgcaccagcg attcggcaat atcgatgtcc agtgtggcga
      541 ggatattctc gcggacgagg tcgacctcgc cgcgccggat accctccttg atcaggcgga
      601 cactggtatc gaaaaattga gcgttgatcc tctgggcgct ggtgctggct ttcttcatga
      661 cctcgatcgc gtggtagaaa tcgcccatcc gggagagggc gtcggtgatc tgccggtaga
      721 gctgggccgc cttcgttgcg tcacaggact catcgatcag cggcatgatg tcgaggagga
      781 tggtcgagtt gccgctcttc tcgacgaccg caatcccgga gaggacgatc tcctcgatgg
      841 ggagtttggc ctccaccgtg ctccgggtgt tgaactcgaa cgccttctca aggtaccagc
      901 gttctccgct ccgctccgct ttcttcagga gctcgagtat gagggtctgg cccgcccacg
      961 gcctctcgtc gtcgatcctg atcagcctgg cgtagacctg tttcttgtcc cgctgacggt
     1021 cgaggagttg ctcggtcagg atcgcgagga cctcggtgag ccgttcctcc gggacatccg
     1081 ggagggcatc tatgatctgc tcgatatcag cgatctcctt cttttgcgac gacttccacg
     1141 tggcatccac gatgtcggcg atggagttgg tgcgccggat cttctggttg atctcggtgg
     1201 cgctcgatag tccgctgatc acgagggcga tgttgccgga ctcgatgccg gaaccggcaa
     1261 tcgcccgggc cacgtcggca tggagctgtg actgtcttga tatatcgccg atctcggaga
     1321 gcatgaggag ggcatgtcgc atgatatcga tattctggta tttctgtccg tattggatga
     1381 ggagcggtat gatctcggag aggattgccg agcgatattt acggatgctg atcttatcga
     1441 acgcttctcc tcccttctcg atatagtgga tatctccggt atcgactccg gtctggacga
     1501 ggctgcggga gatctcggcg agtatgtcgg actggtggct cttgcggtcg agcttattga
     1561 ccagggagaa gatggcacca aaccagatgt cgtctttatt ctggctataa agccctgcgg
     1621 cacgtcttac gaggtcaaat atttcttctt tcgtcggttc gacgagctgg ctcaagagtt
     1681 cgggctgtcc cccctcgaag gctgccgtta ccagggcggc attcatctgc tcatagagcg
     1741 atttcttctc tttccgtgtc tgggtctccg atatgtccag cactaccttt tcaagctcac
     1801 gcggatcccc ggaagagatt gcggaggtga tcttctgttt catctggggt tttccatcca
     1861 ttttgatcag cgctctccag tgggttctgc atcatgcatg tccattatca gatatataga
     1921 ttgcccccca cccacaagaa tgtttttcaa ggggaatcgg ggagatcctg aaaaatgtcg
     1981 gagatatggc ggacgataca ggcccgccgt gtcaccgtga atggaacact taatgtgtga
     2041 tggtttcatc tgttcccggg ggtcacgcgg gcgggtgatc acacccgccc gacgacctgc
     2101 acgatctccc ggtacatggc gtccgcgata tccggatccc ttgactcgat catgaccctg
     2161 atcatcggct ccgtgcccga tgcccgcaca agcgcccagg cgtcgtccct gacgatcttg
     2221 atgccgtcga tcttctcgat ggcctccccc gcgaacgctt cctcgatggc acggaccagt
     2281 gcggccgggt cgtgggtgtg gtgcttctcc ttgaccaggt ggtagagggg gaggtcaccc
     2341 tggatatccg agagcctcct gccgttatgg cttgcaagga ccgccaccat catggcggcg
     2401 gtcatgcccc cgtcccggca gaactggtgg tcggagtata tcagcccgcc gtttccttct
     2461 cccccgaacg agaccttttt tcccccgccg atgagctcga tcatcctcct tgcgacgtag
     2521 atgcttccca cgggggtgta gtcgacggtg cacccgtgtt tctccgcgat atcccggatc
     2581 agccgggatg tggcgacagg ggtgacgacg agcccgccgc cgttcctggc gcagacgtaa
     2641 tcttcgatca gcccgaactc gtagttctct tctatatagc atcctttgtt atcaacgaag
     2701 accgcccggt cggcgtcgcc gtcgtgcgcc actccaaagt cggcgcccgt cgcgacgacc
     2761 atctctgaga ggggccgcag gccttcgggg gtcggctcgg gcatccggcc cgggaaggtg
     2821 ccgtcgagac gggcgttgat ggtgtggacc cggcagccca tcctcgagag gatgatcggg
     2881 gtggtgagcg ccgcgggccc ggagccggga tcgacgacga cggtcatccc ctccccgatg
     2941 cccgccggga agtgccgtac gacggcctcg atgtactcct cgagccggtc gggagcggcg
     3001 gtctccgcgc cgacgccttc ccaggaggcc gcctcgaact cgccggcaaa gaaccggtcc
     3061 tcgagccgga tgatctcttc gtcggacatc tcggtgccgt cggcctcgat gaccttcacg
     3121 ccgttgtact cgggcgggtt gtgggatgcg gtgatcatag cgccgccgct aaaccggccg
     3181 gtcttgatga tgtactgcag ggccggcgtg gggaggatgc ccatgtccac cacgtcgcac
     3241 ccggtcatga ggagcccggc cttgagggcg tgagccaggg cctcgccgga cgtccgtgta
     3301 tctctcccga cggctatcgt gccctttctc atcgatccga gcgctgcgcc tatcttgagg
     3361 acgagggccg gcgtcatggt ctctccgacg acgccccgca cgccgttcgt cccgaacatc
     3421 tgtttcttct tctgcattcc gtcacttctc cacgtcgtgt agttcccgtt tcagccgatc
     3481 gatcgccctg atagcggcgg cgaacgcctc ctcgcggtgt tctgccagga gagcgatata
     3541 ggttatatct tctcccgcat agagtcgccc tttccggtgg taaaaccgtg cgccgatgat
     3601 ccccgggacc gcttccatct ccctcctgag gctctcgacc agggcgtcga cggtgtcgtc
     3661 gaagtccatg tgatcggtgc gttcggtgcc ggtccactcc ctgacgatcc cgttgaacgt
     3721 gaggatggcc ccggctcgcg agacaccgca ctcgcgtttc agttccctga ccagcccttc
     3781 gatggtgtag tggtcttcga acttcggaag cgcggcgatc acctcgtcga cggtggggtt
     3841 ccggagcacg acgttctcac ttgcaaggtc gcctatcacg actcgcggga agagccggga
     3901 cttgaaccct tcgagtatgg catactcgac cccggcgttg cagaggaggt ccagcgtgga
     3961 gtcaaggtcg ttctcgcgcc tgacgacgac cgacttcgtc tcgtcgacac cgccggatag
     4021 agcagcatgc gattcatgga acagggtggt gtccttctcg ggctcaagct gaaagccgtg
     4081 gtgtcccagg tgcttgatgg ccccgacggt cccgcgtgcg gagagcgccg ggatgaggtt
     4141 cctgatgaac gtggtcttcc cggagttcga ccggccgaca atctggatga tcttcatggt
     4201 tcttcttctc ctgttgggtt ctctttctca tcttcctggc cggggcggga cttcaggatg
     4261 accgatgccg cctcggccac gccccgcatc acaccggtga tccggtcctc gacgtcgatc
     4321 tcgtcgtcga tgttcaggct tgtcccctcc acctcgagca ggaaccgggc gacctcgctt
     4381 gcctcaaaga ggatctcgtc gagttcctct gcggtgaaga acccgcacat ggctccgtag
     4441 aagaacgtgg cccccgcttt cttcaccttc ttcttgaacg agctccgcgc ctggttgacc
     4501 gcctgcggcg tgtaggagtc gaccatgaac gggcagaggt cggggaggtt ctcgccgatg
     4561 aacgtcatct ccgagccgct ggtggtcttg aagtctgcgc agagccgtgc gatcgcccat
     4621 tcccgggcgg tgatgtgggt gtgtttgcgc aggaactggt tgacccgctg gtaggtggcg
     4681 ccgtccactt tcttgaactt tctgtatttg tttgggtctt tgcggttgtc aggctcttcc
     4741 atttcttatc ctcttgtcgg ttttgctatt tcaatagggc tggtcgaacg ccggcgcacc
     4801 ccggccgccc gccgcgtcaa gagagcgggg tcgccacggc gatccggcgg gcgctatgcc
     4861 cgatgggccc tgccccttca atgtatagca tcatgcggtt tatacctgcc tctttgatcc
     4921 cggggtgcgg atggatcttc ggcctctccg gaggagctct ctcccgatcg aggcctgttc
     4981 tgcggtctat ccttccccct ggagattgcc gggattaccg gcgaggccgc cctgcgcagg
     5041 ggctcgtgac ccccagggtt ccttcctccc cacgtcggga atccgctccg ttttgctccg
     5101 gggagttccg gtcgcaggtg cgccttcgtt cggagaaaag gtttgcgggg ccaaagagcc
     5161 gtctcccggc gggaccggcg tcatgggcgt accgggctat ccgatctttc cgggtatgcg
     5221 agggagagat tgccggcgac ctatggtaac cgcgagcctc gatccgtggc aggaggccgg
     5281 agcgtatgcg ggaaggatat gtgagatccg tggatttcag gtggcatgaa gcggtatctg
     5341 ttccggtttt cggaaccttc gtatcgtggt tataagcccg gatcgtgagc cccggcatgg
     5401 gtaaggtcgt ctccctggac gttcgggcgt ttaaaagcat ctatgcggat cgtttatatg
     5461 tgggcgggtt ctgccggctc cgtggcaccg gtggcgttcc cgcatcgttc actttcaccc
     5521 cgcacggtat ggggttcata taccctggag acgatgtatt catcagggtc tgcctcccgg
     5581 cccacccggg gagcagcccc atacctccta actccgatac ctcctgtatc acccgaaata
     5641 tcttttttgt ggcctgaagc accatgaacc gcctttaccg tttgccggac ggcggagcgc
     5701 ctttcatcca ggaacggggt ttcccggtgc cggcaggacc ggcaccgggt tgtcccgacg
     5761 accactgctg aggcgccggt tgttgcccgg ttccgggccc cgttcggcgt tgtacgggtc
     5821 gggtccccct ctccgggggt ggtgcacacc cctatcgatc ccctgcgatc gtggccggcc
     5881 atccccggac cggggccggg caggggtttt tgaggtgaga tctctattgc ggggaatagt
     5941 gatttaccgc aatcttgata ttcttcgata tctaacccat tatccgatgg aaaaaacgcc
     6001 cttctcggaa tggctggatc gtttcagcaa ctacctccgg atgcggaact actcgccccg
     6061 gaccatccgg aaatacgagc agaccatcga gcgttttgcc cggtatgcct ggttccggga
     6121 gcattcgggt ccgggcccgt tcgatgagac ggcgtgcggg gatgcccccc tggatgcgga
     6181 cgtcaacgtc tctgcggccc tcgtcaccga tttcttctcc gccctctcgg aagaacgtga
     6241 ctataagccg aagactctcc accggatgat atcgacgctc tcctcgttct accgctatct
     6301 ctacgcacag ggggccgttg tcgcaaaccc catgcccggt gtggagcggc cccgggtcaa
     6361 gaaccaggaa ctgaagtacc tcaagcacag ccaggtggtc cgcctgctca acaccatccc
     6421 ggccgggcgg gaccggctga tcgtccgcct catatacgcc accggcgtcc gtgtttcaga
     6481 actctgcgcg ataaacgtcg aggatatcga ctttgaggag cagacgatcc gggtgaaggg
     6541 gaagggcgac aagttccgga cggtcttcgt cgacgaggag acgctcggcg agatcgagcg
     6601 gttcgtcggg aactcaatcg agggcccgct ctttttgggc cagcagggca accacatctc
     6661 cccgcgaacc gtccagcacc tcttccggca gtatgctccg ccggggatta cgccgcacaa
     6721 gatccgccat tcctacgcaa gcgaactcta ccggcgctca aagaacctcc gcgtcgtgca
     6781 ggagaacctg ggccactctt ccatcaaaac gaccgagatc tacctgcata ccgacatcga
     6841 agagcggaga agcgtctacc ggcagtattt cccgctctcg aacgggaaga agatagacga
     6901 gtagccggcg gtcgataaac cgcgtgatgc tatatgggtt ggaggtggcg ggggatccat
     6961 ccggcccggt tcttcgggag agctcaccgg aggctctcct cctcccacgg ccccgggaaa
     7021 tgggtgcagt cgcccggcac gagaggcttt ctccccccgt tgccggcgga agtttgagcg
     7081 ccagacggat tacggctgca ggtacccggc tgccgaactt cgggtccttc gcggcttcgc
     7141 gcccgggtat acgagcgata aaggtagcct ctttgcatgg agtgcgagat gcgattagaa
     7201 ggatcagggc ccatcgtctt tcgccctccc gaccggcaac gctcaaattg caggagcacc
     7261 taccgtattc gtatgcgcta catcgtcaca ggcggagcgg gattcatcgg ttcgaacctt
     7321 gcggagcacc tggcacgcga ccacgacgtc gtgatcatcg ataacctcag ctccgggcgg
     7381 cgggagaata tcgagcacct catcggtcat ccccgggtga cgttcataga ggggagcgtc
     7441 accgaccttg ccctgctcac cggtgccttt gccggcgcgg acggcatatt ccatcagggg
     7501 gccatcgcct cggtcccccg ctccgtggaa aagcccctgg agacgaacgc ggtgaatgtg
     7561 ggcggaaccg tgaacgtcct ctgggccgca aaggagtgcg gcgtcccggc ggttgtggcg
     7621 gcctctacat cggcgatcta cggcgacgac ccggtctttc ccaaacacga gacgatggcg
     7681 ccgacgccgc tctccccgta cgcggtctca aagctcgcgg gcgagtacta cgggaaggtc
     7741 ttctccgacc tctacggcat caggacggta ttcttacgct acttcaacgt ctttggcccc
     7801 cggcaggacc cgaactcgga gtacgcggcg gtgattccga agttcatcac ccgcctgctc
     7861 gccggaaaac cgccgatcat ctacggcgac ggggaacaga cccgcgactt catctttgtc
     7921 gccgacgtgg tgcgggcgaa cgtccaggcg atggagggca acgccaccgg cgtcttcaac
     7981 atagccgggg gccgccggat cagcctcaac gagctcgctg cccgcctctc ggggatcgcc
     8041 ggcatgcacg ccaggccgct ctacggaccg gtgcggccgg gggacgtgcg tgactcgctt
     8101 gccgacatat cgcgggccag ggatgctttt ggcttttctc cccggagcac ggtcgacgaa
     8161 gggctccgcg agaccgtggc gtggttccgg gaccggggtt ctctataacc cttcttctcc
     8221 ggcggtcggc ccccccgttc ccggcatggt gggtgcaggt atctatatat cttcgctcca
     8281 ccgtatgcgt gcgaggtgcc aatgtatgcc ccggctgatt acaccggaga ccccccgcaa
     8341 gggtgacgac agatatgctt cacgggcgta cagcgatatg acagcaacgg atctgcacga
     8401 tcccgcggtc ctgaacaaca ggggcgtggc gctcgagcgg cagggccgtt acgaggaggc
     8461 cctggccgcg ttcagtgcgg ccctcctctg cgacgacgac gacgtctacg cctggaacaa
     8521 ccgggcagtg atcctcaccc gcctcggccg cccccaggaa gccctgcttg catgcaaaga
     8581 ggcgctcgcg gtcgaccggt catgcatctt cgcctgggtc acctacggca cggtcctcgg
     8641 caggattgga agatacgtgg aggccgagtc ggcctttgcg gtcgcagagg acctcgaccc
     8701 gcaggcgaga gcgctctacc ccgggaactc gacgatgacc cgggcgtgag ggatcgcgtc
     8761 gccctgcccg cgatacaccg cccggtgcga tacggagggg tgccggcaga tccgatcgat
     8821 cgctctcccg aactgctcgc acgacatcgc cgggcctcca ccgaaaaaac cctacgcttt
     8881 tatcctccat accgcaatag agtaatcaca atcgcgtgcc ccggcaccgg agtcgatcct
     8941 gatgaagacg ctcattcttg caggcggaag cggcacccgc ctcttcccgc tctcaaggga
     9001 acactaccca aaacagttca tcccgctctt cgacaacgaa tcgctcttcc agaagacgat
     9061 aaagagggcg ctcctcttct cctccccgca ggacatcgcc atcgtcacca ataaggagca
     9121 ccggttcctg gtccgggacc agcttgccgc gatcgggtgc aactcaaagg tcatcgagga
     9181 gccggtgggc aggaacaccc tgccggcgat ctactacggc gtccgggagg tcgcccgggg
     9241 cgaaccggag acgatcgcgg tgctgccctc ggaccacctg atcaccgcaa ccggatcgtt
     9301 tcttgcggcc ttccgccggg cggagcacct cacgaaagac tacctggtgg tctttggagt
     9361 gcggccgacg tcgccccaca ccggctacgg ctacatccgc ccgggcgact ccctcgggga
     9421 cggttcgctc gtggatgcct tcgtggagaa acccgacctt gcgaccgccg ggaggtacgt
     9481 cgccgacggc tacctctgga actccggcat gttctgtttc gatgccggcc tcttccttgc
     9541 cgagtgcgag acgtgcgcgc cggacgtggt ccgggcgttc gagcggcccg ccgatgaggc
     9601 ataccatgcc gtcccctccc tctccatcga ctacggtatc atggagaaga cccggcgggc
     9661 ggctgtcgtg ccgcttgagt gcgactggaa cgacgtgggg aacttcgacg cgctttacgc
     9721 cgccctgcca aagaacggag acaaaaacgc cgtcaggggc gaacatatcg gtatcgactc
     9781 ctccgagaac ctgatcatag ccgaccgcct ggtcgccacc gtcggcgtcc gcgaccttgc
     9841 catcgtcgag acgaaggatg cgatcctcgt cgccgcccga aacgaggcgc agcgggtggg
     9901 cgagatagca aaaaccctcc gcgagaaggg cgactcccgt gcgctcttcc acacgcaggt
     9961 ccaccggccc tggggttcgt acacgaacct cgaggagggg cggtcctata agatcaagcg
    10021 ggtcaccgtc cccccgaagc gtcgcctctc gctgcagatg caccaccacc gctccgagca
    10081 ctgggtggtc gtcaccgggt gcgccgaggt gacgatcgcc gataagacct acctcctccg
    10141 gaacggcgag tccaccttcg tcccggcggg gacggtccac cgcctcgcga accccggtct
    10201 cctgccgctc gaactgatcg aggtgcagat cggggagtac accggtgagg acgacatcgt
    10261 caggttcgaa gacgacttcg agcgggcgtg agcggtggat attccacccc cctgcccgcg
    10321 accaaatcga tataatcccg ggatgcatag tacataaggg ccggtacccc aggtatcagc
    10381 gattggattc
//
LOCUS       DAOSEE010000079        10388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_2449_length_10388_cov_262.475370, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000079 DAOSEE010000000
VERSION     DAOSEE010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 10388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10388
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2449_length_10388_cov_262.475370"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..882
                     /locus_tag="PLI76_07125"
     CDS             43..882
                     /locus_tag="PLI76_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61840.1"
                     /translation="MKMKTNTTPPGSRSRLWMAVPAIAFGGIGIELLLMSAGFPYAAL
                     AGIAGCVIASFILFYQAYLKPRRDIVSLFVPLYAVLIFIVPNDISSGVIVQAFYAATI
                     TLLAVRLEKLFNAPKQEKKTMKQMLNEYIARIEPFLAPIDEETGHLVAQALLTYKFEL
                     YGNAAGKIAEALARLDTIAPHPGTLERALLILRERAGGFAESRVITSPEHTFMKEDYD
                     ALAIRLRPDQIESPAALDLDNALILLYAVGIETSPDDEQALEEHQRFVIQVLESYKDK
                     LTA"
     gene            1214..1861
                     /locus_tag="PLI76_07130"
     CDS             1214..1861
                     /locus_tag="PLI76_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HOI61841.1"
                     /translation="MKSELAHEIERLTSLARERGADARQIAAHDVVVAEWVRFKCRFG
                     CKGYGKHMSCPPYAPTPTETRRLLAEYSTGILLRFEGIPGHPDLKPDDIPLDFHPFLR
                     DLILWVSSTVHLLEKTAFYDDFPKAFGFGAYPCIYCEHQHCVAEEHEGVVDESIRRLC
                     RHMDLVRPTMEGAGIDVFATARKAGWDLHIIPCKDLEYGKIVHGNITSIGLVLIE"
     gene            complement(1874..2977)
                     /locus_tag="PLI76_07135"
     CDS             complement(1874..2977)
                     /locus_tag="PLI76_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HOI61842.1"
                     /translation="MDSIRIAIVGIGNCASSLLQGIEYYRGRGKVDAVGLMHQDICGY
                     RPSDISVAAAFDIDARKVGKDVAEAIFSPPNCTTAFCPGVPETGVTVRMGRVLDGFPE
                     HMRDYREETRFVLADEEEPSREEIVRALEDSGAEMLLNYLPVGSEEAARFYAGCALEA
                     GIGFINNMPVFIASDPAWAERFRARGIPVIGDDVKAQLGATITHRALVDLFRRRGVRL
                     DRTYQLNTGGNTDFLTMLNRDRLASKRTSKTEAVQSVLDAPLDDEDIHIGPSDYVCWQ
                     KDNKVCFLRMEGRLFGDVPMQIDLRLSVEDSPNSAGIVIDAVRCCRVALDRGIGGPLV
                     SPSAYFMKHPPVQIRDDDAFRMTEEFIRGIRND"
     gene            4125..5057
                     /locus_tag="PLI76_07140"
     CDS             4125..5057
                     /locus_tag="PLI76_07140"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOI61843.1"
                     /translation="MADRATGELRAGSFAVVVPAYNEGLVIGSVVLQAKRHTDRVIVV
                     DDGSADRTAAIARLAGADVIQLKKNGGKARAMMAGFARARDLEYDTVVMLDGDGQHDP
                     DEIPIVVAPVLAGEADLVIGSRFLETGADIPVYRRAGQLVLTRFTNLSADGGNFVTTD
                     SQSGFRALSRRALENLTFASEGYNIESDMIAYLAPLDLKVTEVPITVTYDVPHKHKKN
                     PISHGFGVLARIVEVIGHRRPLLIFGIPGGIIALFGIGLEIYTFSVFFRTGQIHYVAV
                     IFGITALILGLLLISSGLVLNFLVSMMKRHISEA"
     gene            complement(5184..5981)
                     /locus_tag="PLI76_07145"
     CDS             complement(5184..5981)
                     /locus_tag="PLI76_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61844.1"
                     /translation="MKIKEYNHYRAGAYLLTGFAPLLILAALLTDPRGYLQSPFLMLS
                     ATCFIGGILLFALGGGDSVDTRLASRLSMQGILALGDIILDLGGDGAAIFLPPENDGG
                     RVMQFIPIRSTCRSYQADGGGFTHHSGSVGTLNPPLAATILDDLKRDNDLILPREYSL
                     LMGAIREVCEDLLSVTGRVEVRRKGDTVTLDLHNYLLLSACTHRREVSPASCTLCPCS
                     ICSLIACMIAEGLGCEVSLSQVALDETARPPLMRVHYTLMEGAGIPD"
     gene            complement(6029..6295)
                     /locus_tag="PLI76_07150"
     CDS             complement(6029..6295)
                     /locus_tag="PLI76_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61845.1"
                     /translation="MRKKDIASVVLMVWMLAMSNLMGILYGPDLRFFIAVALMGFFIV
                     AYTMRPIFSRPDYIRNIDRMTVAGAALFGLIIYLRMLELIGYWS"
     gene            6536..8035
                     /locus_tag="PLI76_07155"
     CDS             6536..8035
                     /locus_tag="PLI76_07155"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HOI61846.1"
                     /translation="MPGFFSNVFKLATGTMLAQVAGITLIPVVTRIYSPECLGVLQLF
                     LSIATVIVIVSSLSYNLAVMLPEKDEDSINIFVLSIMCVTGISVATGAVVVGFADRIG
                     EIFTTAAIADYLIWLPVFVILSGFSLILNEWLSRKVKYGVLSRSIVVSSISTRMFQIG
                     GGLMTASPLGLILGSVMGLALANFFMFRGLKEDIGLLKAVTVQRMKDLAIRYREFSLF
                     GTAASLANSMSWELPAFMLGLFFSSTILGYYALAMMAVRVPMMMVGTAVSQVFFQKAS
                     EEMNLTGGVQTVVQEIHTRLISISIFPFVIFIVLSEGLFTFIFGANWLIAGTYARILA
                     PWLFAVFIISPISSLFGVMERQKAYLFFESVTLCTWALIFFIGGTSGDPIFTLALFSI
                     CGMLIWGSKSMYLIKASGAGYRGSAESLARHLLLSIIVSLPLIFGVYMGLAFLFLLGI
                     AGITAIVYYLTIFFTDALIQREIKELAIGFFRPEHIDLIKRLSLFRYWN"
     gene            complement(8099..9112)
                     /locus_tag="PLI76_07160"
     CDS             complement(8099..9112)
                     /locus_tag="PLI76_07160"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOI61847.1"
                     /translation="MARLDVRRLEGDEFHAWDALAAGSAQGTVFHSSDWLVKNASLQD
                     QDLILLGCYDSEELIGGCPLFLSRPYNLLGLAISTAALTPYGGVMMTESERAKQRARE
                     MYADRVITALHEYILRQGFDYVSLVNSPGLTDIRAFTRRGWSPAIYYTHILRLHDDIL
                     KTVSKDVRQNIRKARKQGISSTERFDPGVFWDLTVRTFSKQGKQPPVSERHLTGVLTM
                     IREKGIGSMRIASTSSGEIAAAEVILWDEKMAHSWSAVAAPEHLSTGAVSLLCYDIFT
                     SLKERDFRAINLMTGNIPRLSAFISGFNPGLVPYYGVDYSRFRYRIFKRLQQYVYRDA
                     SPG"
     gene            complement(9376..>10388)
                     /locus_tag="PLI76_07165"
     CDS             complement(9376..>10388)
                     /locus_tag="PLI76_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOI61848.1"
                     /translation="EIWELFTRKEEYDNHSRDHHDRFLYSMSRHGDISLPRASGVLIE
                     KGYSCEYPETRPFAICLTHDIDTVYRPLRSKILDILCALKSGDLTQAGYIAPQLCSKK
                     RPEWNFSEIVDLEASYGACSSFYFLALEEGDRDYAYAIEDLEQDMGTLLDRGWDIGLH
                     GGCEAYRDSEQLQREKRYLERILNRTVDGYRNHFLKFRVPDTWELLEQAGFRYDTTLG
                     YPDSIGFRNGMCHPFQPFNIHTNREMEILEIPLAVMDRTLFSYMQLNMEQAWARMERI
                     LEAVERYNGVITILWHNVFMTGAPLRIYRKILEYGCEKGAWMTSCREIEAWWRKGGMQ
                     PP"
BASE COUNT         2102 a         3095 c         2969 g         2222 t
ORIGIN      
        1 cggcattcct ccccggcctg aaggccgggg tctcctgcct atatgaagat gaagacaaac
       61 acgaccccac ccgggtcccg gtcgcgcctc tggatggcgg tccccgcaat cgcgttcgga
      121 gggatcggga tcgagttgct cctgatgtct gccgggtttc cctacgcagc cctggcgggt
      181 attgcaggat gcgtgatcgc ctcattcatc ctcttctacc aggcatacct caaaccgcgc
      241 agggatatcg tctcgctctt tgtgccgctc tatgcagtcc ttatcttcat tgtgccaaac
      301 gatatcagtt cgggcgtgat cgtgcaggcg ttctacgcgg cgacgatcac ccttcttgct
      361 gtacggctgg agaagttgtt taatgcacca aaacaagaga agaagacaat gaagcagatg
      421 ctgaacgagt atatcgcccg gatcgaaccg ttcctcgccc cgatcgatga ggagaccggg
      481 caccttgtcg cgcaggccct gttgacgtat aagttcgagc tctacgggaa tgcggccggg
      541 aagatcgcgg aggcgcttgc tcgactcgac acgatcgccc cgcatcccgg aacgctggag
      601 cgggcgctcc tgatcctcag ggagcgtgcc ggcggctttg ccgaatcccg cgtgataaca
      661 agcccggagc ataccttcat gaaggaggac tatgacgctc tcgccatccg cctccgcccc
      721 gaccagattg agagccctgc agcactcgac cttgacaacg cgctgatcct gctctacgcc
      781 gtcggtatcg agacctcccc cgatgacgag caggcactgg aagagcacca gcgctttgtc
      841 atccaggtac tggaatccta caaagataag ttaaccgcat gatcaacctg accgagggta
      901 acctctttcg aggcttcata acagtagcgg tgagagtaca gcgatcggcg tctcgtgcct
      961 gcagacggtc gcgccgttct acgtcctcat cgctgttgca atcgtcctcg ggtttgctct
     1021 caacggggcg ggggataccc ggagaggccc atgtatgcga cgctcttctc catggtcctc
     1081 ctccctcgac gtacccggcg cggggcctgg aaggcgaccg tgatagagcg gtgaggcccg
     1141 caggcgttcg cccccttcac cccacaggat aaatacctgg atgcggtaga tatcctcact
     1201 gagggaacgt gcagtgaaaa gcgaacttgc gcatgagatc gagagactca cctcccttgc
     1261 ccgggagcgc ggcgcggacg cgcgccagat agccgcccac gacgtggtcg tcgcggagtg
     1321 ggtgcggttc aagtgccggt tcgggtgcaa gggctatggg aaacatatgt cctgtccccc
     1381 gtatgccccg acccccaccg agacccggcg actgctcgcc gagtacagca ccggcatcct
     1441 cctccggttc gaaggcatcc ccggccatcc cgacctgaaa cccgacgata taccgcttga
     1501 cttccatccg ttcctccgcg acctcatcct ctgggtgagc tcgacggtgc acctcctcga
     1561 gaagacagcc ttctacgacg atttcccgaa ggcgttcggg ttcggggcat acccctgcat
     1621 atactgcgag caccagcact gcgtcgccga agagcacgaa ggcgtcgtcg acgagagcat
     1681 ccgaaggctc tgccggcaca tggacctcgt ccgcccgacg atggaggggg ccgggatcga
     1741 cgtctttgcc actgcccgga aggccggatg ggacctgcac atcataccct gtaaggatct
     1801 cgagtacggc aagatcgtcc acggcaacat cacgtcgata gggctcgtcc tcatcgagtg
     1861 atgcgcccgg ccttcagtcg ttccggatgc cccggatgaa ctcttcggtc atgcgaaagg
     1921 cgtcgtcgtc cctgatctgc accgggggat gcttcatgaa gtaggcggac ggggatacga
     1981 gtgggccgcc gataccccga tcgagggcga ccctgcagca ccggacggca tcgatgacga
     2041 tccccgcaga gttcggggaa tcctcgacgg agaggcgaag atcgatctgc atggggacat
     2101 ctccaaagag cctcccctcc atccggagga agcagacttt gttgtccttc tgccagcaga
     2161 cgtagtcgct cgggccgatg tggatgtcct cgtcgtccag gggtgcatcg aggaccgact
     2221 gcaccgcctc ggtcttcgag gtcctcttcg aggcgaggcg gtcccggttt aacatggtta
     2281 aaaagtccgt attcccgccg gtgttcaact ggtaggtccg gtcgagtctc accccgcgcc
     2341 gccggaagag gtcgacgagg gcccggtggg tgatcgtcgc gccgagctgg gccttgacgt
     2401 cgtcgccgat gaccgggatg ccccgcgcgc gaaacctctc cgcccaggca ggatcgctcg
     2461 cgatgaagac cggcatgttg ttgatgaaac cgatacccgc ttccagcgca cacccggcat
     2521 agaagcgtgc ggcctcttcc gaccctacgg ggaggtagtt gagcagcatc tcggcgcccg
     2581 aatcctcaag cgcccggacg atctcctcgc gcgacggttc ctcctcgtcg gcgagaacga
     2641 accgggtctc ctctctgtag tcccgcatgt gttcgggaaa gccgtcgagc acccgcccca
     2701 tccggacggt cacaccggtc tccggtacgc cggggcagaa ggcggtggtg cagttcggcg
     2761 gggagaatat cgcttccgca acatcttttc cgaccttcct tgcatcgata tcgaaggctg
     2821 cggccacgct gatatccgac ggccgatagc cacagatgtc ctggtgcatc agaccgactg
     2881 catcgacctt gcctctcccc cggtagtatt cgataccctg gagcagcgaa ctggcacagt
     2941 tcccgatgcc cacaattgca atcctgatcg agtccatgac agtctccctg agatccggtg
     3001 tgtcgctgag cccatcccct ccatcccgga gagtgtgccc ggcaccgcat gagtggacgc
     3061 aaccatgaaa gacaggttgc gttttggact ataatacgga gggggaaata taagggtaat
     3121 ggttcgggaa gcagcagggt aattcgtgcc tcacgatgca aagacggagt agcggtcacc
     3181 catcgccacc ataaagaaga tgatcgcgga ggcggtcgga tacggatgaa cagcgtttga
     3241 gagaacccgg accaaaccgt acaccccctg tacgagcggg agggacgagc cctatcggct
     3301 ataacctcct gcacgatgac catgcccggg gtacgaggca ggagggcgtt cgcggtcgcc
     3361 gttcgccggt cttgaatctc ggtcgaaccg aaacagacga aacagcagac gtccgtcctc
     3421 gatggatggg tttcgtcggc cagaggcgcc ggggaataag agtgaacgcc ctccaggtgg
     3481 tcggaagggg cacgaagaac atatccccca ctctgttccg aatcccgatg cccgatcccc
     3541 ggtcaacgtg aggtgctccg cttcccgggc cggatcaggg aggagaatgc atgcccggta
     3601 tacggttgtt atcggcaaaa tatttatacc aggaacagta tggcacagtg cccgggcgat
     3661 gtgatgaaac aggcagattc tctatatcac tgcacaggcc tgcagtccgg atggccccga
     3721 ccgcaagaag gagggatctg gcattacacc gcaggcgctc aactggagag gtaggtaaaa
     3781 tgcgtcgatc tggctcacca tttcctttca tagccagaat ctctgcttac tggagagaga
     3841 tcaccatccg gggcaggcct tcctgctccc tatctcaccg ggcacccgct ttcaccggca
     3901 gcaccccgga gatggcctgc acctcgactg ccggttgtca tgtaaccccc acgcactggc
     3961 gggcaccggc tgagagtcct gatactacct ctgccgtagc aagtggaagt tggagtcgac
     4021 agggttactc cctgccttct cccgacgcat cggggaatgg aaccggcatc acgggcgaca
     4081 atcaccgctc atcggtcacg ccccggagtg ggttgagctc cggaatggca gaccgggcaa
     4141 ccggggagct ccgtgcaggt tcgtttgccg tcgttgtccc cgcctacaac gagggcctcg
     4201 tcatcggttc cgtcgtcctc caggcaaagc ggcataccga ccgggtcatc gtcgtcgatg
     4261 acggttcggc agaccggacc gccgcgatcg cccggcttgc cggcgccgac gtcatccagc
     4321 ttaagaagaa tggtggtaag gcaagggcga tgatggccgg gtttgcccgg gcgcgggatc
     4381 tcgagtacga taccgtcgtg atgctcgatg gcgatgggca acacgatccc gacgagatcc
     4441 cgatcgtggt ggccccggtt cttgcgggag aggcggatct ggttatcgga tcgcgatttc
     4501 tggagaccgg agcggatatt cctgtttatc gccgggccgg ccagctggtg cttacccggt
     4561 tcacaaacct ctcggctgac ggcggcaact tcgtgacaac cgattcccag tcgggttttc
     4621 gggcgctctc ccgccgggct cttgaaaacc tgacgttcgc gtcagaaggc tacaacatcg
     4681 agtccgatat gatcgcctac ctcgcccctc tggacctcaa ggtgacagag gtcccgatca
     4741 ccgtcaccta cgatgtcccc cacaagcaca agaagaaccc gatctcccat ggtttcgggg
     4801 tgctcgcccg gatcgtggag gttatcggcc atcggcgacc attgctcatc tttggcatcc
     4861 cgggggggat tattgcgttg ttcgggatcg gccttgagat ctatacgttc tccgtgttct
     4921 tccgaacagg ccagatccac tacgttgcag ttatcttcgg gataactgcg ctcatactcg
     4981 gacttttgct gatctcgtcg ggactggtcc tgaacttcct tgtatcgatg atgaaacgcc
     5041 atatcagcga agcatgaata cggtgggagc tgcgcttccg gggttgccgt cgcgcatcca
     5101 ttgcccacac tggagttatc ttcatcccgg tcgcccgggc ggcgcccggt tccctctccc
     5161 ccaaacccgg acggcccgcc ccttcagtcc ggtatccctg ccccctccat gagggtataa
     5221 tgaactctca tgaggggtgg ccgggctgtc tcatccaggg ccacctggct caggctgacc
     5281 tcacacccga gaccctcggc gatcatgcat gcgatcaggc tgcagatcga gcaggggcag
     5341 agcgtgcagg atgcggggga tacctccctg cgatgggtac aggccgagag gagcagatag
     5401 ttgtgcaggt cgagcgttac cgtatcgcct ttcctcctta cctcaacccg accggtgacc
     5461 gagaggaggt cctcgcacac ctcgcggatc gctcccatca gcaggctata ctcacgggga
     5521 aggatgaggt cgttgtcgcg tttcaggtcg tcaaggatgg tggccgcaag gggcgggttc
     5581 agggtcccta cactcccgct gtggtgcgtg aatcctcctc cgtccgcctg ataggatctg
     5641 caggtggacc ggattggaat gaattgcatc actcttccgc cgtcgttctc cggcggaaga
     5701 aagatcgcgg ccccgtcgcc acccagatca aggatgatat cgcccagagc gaggatgccc
     5761 tgcatggaga gccggcttgc cagccgcgta tctacggaat cacctccccc gagcgcaaag
     5821 aggaggattc ccccgataaa acaggtcgcg ctcagcatga gaaaggggct ttgcaggtac
     5881 ccccggggat ccgtgagcag ggccgcaaga atgagcaggg gtgcaaagcc ggtgaggagg
     5941 tatgcgccgg ccctgtagtg attgtattct tttatcttca tgaccatcat tcggatagag
     6001 tctggagtac ggaatacccc ttacctgctc agctccagta tccgatcaac tccagcattc
     6061 tcaggtagat gatcaggcca aagagcgctg caccggctac cgtcatgcga tctatgttgc
     6121 ggatgtagtc cggccttgag aagatcgggc gcatggtgta ggcaacgatg aagaacccca
     6181 tgagcgcaac ggctatgaaa aacctcaagt ccgggccgta gagtatgccc atgaggttac
     6241 tcatggcgag catccagacc atgagaacga ccgacgctat atccttttta cgcattgccg
     6301 gatctattca gacttgccca tatcacgtat aatacctgcg ccggcctgag caggtccggg
     6361 ggcgaggtct gccgttcggg atgactggcg tttgggtgtt cgtttcccga catagggggt
     6421 ctcccgtggc cgggatagag ggatctgccg gctggacacc gttcataccc catatcctgc
     6481 cggtggtcaa gcacaacata tatcacggtt cccgggtgta ggtgggggag aactcatgcc
     6541 gggcttcttc agcaatgtct ttaaactggc gacgggtacc atgcttgctc aggtagccgg
     6601 catcactctc atcccggtgg tcacccgtat ctactctccg gaatgccttg gggttttaca
     6661 gctctttctc tccatcgcaa cggtgatcgt gatcgtctcc tcgctctctt acaatctcgc
     6721 cgttatgttg cctgaaaagg atgaagattc cataaatatc tttgtactct ctatcatgtg
     6781 tgtaacgggc atctccgtcg ccaccggcgc tgttgttgtc gggtttgctg ataggatcgg
     6841 ggagatcttt acaacggcag ccatcgccga ctatctcata tggctgccgg tctttgtcat
     6901 cctcagcgga ttttcgctga tcctgaacga atggctctcc cggaaagtga agtatggggt
     6961 gctctcaaga agcatcgtgg tgagttcgat ctcaaccagg atgttccaga tcgggggcgg
     7021 gttgatgacg gcatccccgc tcgggctcat tctcggttcg gtgatggggc tggctctcgc
     7081 caatttcttt atgttccggg gattaaagga ggatattgga ctcttaaagg ccgtcaccgt
     7141 gcaacgcatg aaagatctcg ctatccggta cagggaattc tccctctttg gaaccgccgc
     7201 aagtcttgca aacagtatgt cctgggagct acctgccttc atgctcggtc tcttcttcag
     7261 ctccaccatt ctcgggtact acgccctcgc tatgatggcg gtgagagtgc ctatgatgat
     7321 ggtggggacg gccgtttcgc aggtgttctt tcagaaagca agcgaggaga tgaatcttac
     7381 aggaggtgtg cagaccgtcg tccaggagat ccacacacgt ctcatctcta tcagcatctt
     7441 ccctttcgtc attttcatcg tcctttccga gggtcttttt acctttatct tcggtgcaaa
     7501 ctggctcatc gccggcacgt acgcacggat acttgccccg tggctctttg ccgtctttat
     7561 catctcacct atttcatccc tgttcggtgt catggaaaga cagaaggcgt atttattctt
     7621 cgagagtgtg accctgtgta catgggcgct catcttcttc atcggcggga ccagcggaga
     7681 tcccatcttc acgcttgctc tcttctccat ctgcgggatg ctgatatggg gttccaagag
     7741 catgtatctc ataaaggcat ccggggccgg ataccggggc agcgccgaga gcctggcccg
     7801 acacctgctc ctgagcatta tcgtatccct ccccctcatc ttcggggtgt atatgggact
     7861 cgcattccta ttcctgctcg ggattgcagg aataaccgcg atcgtctatt atctcacgat
     7921 attcttcacc gatgccctga tccagagaga gatcaaggaa ctcgctatag gattctttcg
     7981 cccggagcac atcgacctga tcaagcggct aagcctgttc cgatactgga actgacggga
     8041 accgatggat acccttatcc gcagtaacgt cagccgatcg gccgcgacgg cacggtaact
     8101 atccgggaga agcatcccgg tagacgtatt gttgcagccg tttgaagata cggtacctga
     8161 accgggaata gtctacgcca tagtagggaa caagcccggg gttgaatccg gagatgaatg
     8221 cactcaggcg ggggatgttt cccgtcatca gattgattgc tctgaaatct cgctctttga
     8281 gcgaggtaaa gatgtcatag cagaggagcg agaccgctcc ggtagagagg tgttccgggg
     8341 ccgctacagc ggaccagctg tgtgccatct tctcatccca gaggatgact tcggcagcgg
     8401 ctatctctcc ggaggatgtg ctggcaatcc gcatcgatcc gatccccttc tcccggatca
     8461 tggtcagtac gcctgtaaga tgcctctcgg agaccggggg ttgtttaccc tgttttgaaa
     8521 atgtgcgcac ggtaagatcc cagaagacgc cgggatcaaa tctctcggtt gaactgatac
     8581 cctgttttcg ggccttgcga atattctgcc gcacgtcttt tgagacggtc ttaaggatat
     8641 cgtcatgcaa ccggagtata tgagtatagt agatcgccgg gctccatccc cttctggtaa
     8701 acgcccgaat atcggtaagg cccggggagt ttaccaggct cacgtaatcg aatccctgcc
     8761 gaaggatata ctcgtgaagc gcggtgatga cccggtctgc gtacatctcc ctcgccctct
     8821 gttttgcacg ctcgctctcg gtcatcatca ccccaccgta gggggtcagg gccgcggttg
     8881 agatagccag cccgagaagg ttgtaagggc gtgacaggaa gaggggacaa cccccgatca
     8941 actcttcact gtcgtagcac cccagcagaa tcaggtcctg gtcctgcagg gatgcattct
     9001 taaccagcca gtcactcgaa tgaaagacgg tcccctgtgc cgagccggcg gccagcgcat
     9061 cccacgcatg aaactcgtcg ccctcgagcc tccttacatc cagtcgtgcc atacggcgaa
     9121 ccgcccggct cgcatggggg gtacccacgg ttaagtgttt cggctccccg tgagcatctt
     9181 ctttatccgg caaaacgcca gtgccggctt tgaagagagg accagcacag agtggatagg
     9241 cacatgtccg gtcccgggca gtgttttact gcagagcggg attggcggac gttgtccggg
     9301 ggtcagtcag gggacctgtt ccgggttatc cgccgggaaa cacccggaga tctcgatgcc
     9361 ggacgcgttg cagattcaag ggggctgcat gccgcccttc cgccaccacg cttcaatctc
     9421 gcggcagctt gtcatccagg cacccttctc acagccgtac tccagtatct tccggtagat
     9481 ccggagtggc gcaccggtca tgaacacgtt gtgccagaga atggtaatca caccgttgta
     9541 gcgttcgacc gcctcaagga tgcgttccat ccgggcccag gcctgctcca tattcaattg
     9601 catgtaggag aacagtgtcc gatccatcac tgcgagcgga atctccagga tctccatctc
     9661 ccggttggta tgtatgttga acggctggaa tgggtggcac atcccattcc gaaacccgat
     9721 ggagtccgga tacccgaggg tggtatcgta gcggaatccc gcctgttcca gcagctccca
     9781 ggtatccgga actcggaact tgagaaaatg atttcgatat ccatctaccg tccggttcag
     9841 gattctctcc aggtatcgtt tttctctctg caactgttcg gagtcgcgat aggcctcaca
     9901 ccctccatgc aggccgatat cccaccccct atccagaagc gtgcccatat cctgctccag
     9961 atcctcgatc gcgtacgcgt aatccctgtc cccctcttca agggccagga aatagaaact
    10021 ggaacatgcg ccataggatg cctcaagatc aacgatctcg ctgaagttcc actccgggcg
    10081 cttcttcgag cacagttggg gagcaatata accggcctga gtgagatcac cactcttcaa
    10141 tgcacatagg atatccagaa ttttggaacg gaggggtcgg tacaccgtat cgatatcgtg
    10201 cgtcaggcag atcgcaaaag gccgggtttc cggatattca caggaataac ccttctctat
    10261 cagcacgcct gatgcccggg gcaaagatat gtcaccgtga cgactcatgg aatagaggaa
    10321 ccgatcatgg tggtcacggc tatggttatc atactcttcc tttctggtaa acaactccca
    10381 gatctcgt
//
LOCUS       DAOSEE010000080        10042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_2540_length_10042_cov_244.507360, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000080 DAOSEE010000000
VERSION     DAOSEE010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 10042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10042
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2540_length_10042_cov_244.507360"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="PLI76_07170"
     CDS             complement(<1..422)
                     /locus_tag="PLI76_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOI61849.1"
                     /translation="MVQILELIRDDHDFIRNLFDELESNPDTRDIRCITLRRELPGHM
                     DAEEATLYARLRGAKPEVVRRLMADHTAIRETLARFEVIPLRDDAWLPALAGLREEIE
                     AHFTLEEEDIFEQARRYIDINTLFELGEVFPREKVAAAR"
     gene            complement(526..978)
                     /locus_tag="PLI76_07175"
     CDS             complement(526..978)
                     /locus_tag="PLI76_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOI61850.1"
                     /translation="MAQSIIDTLKEEHKMVLSELAELSEKGTSNREQKYSTMKESLLP
                     HLVGEEKVLYPRTREEPEMRDISLEGIEEHGAVKTLLSQLDSASTAEEDVWVAKMRVI
                     QENVAHHIREEEDQLFPAMQKKMSSDELSTLNTRYEDAKKAVLPVAPR"
     gene            complement(1062..1511)
                     /locus_tag="PLI76_07180"
     CDS             complement(1062..1511)
                     /locus_tag="PLI76_07180"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOI61851.1"
                     /translation="MGQNILDLLRREHAKVLAGLDELQRKGISDRADKFNRMKNELLP
                     HMAGEERVFYPELEARGLSDLMAVSRGEHKAAREIVDRLNGIPMADEDDWVRVVPELR
                     EAMRNHIEREEKMVFSEARRTMDDALLLHIGGRFEEAKGKAHLRPLR"
     gene            1627..2940
                     /locus_tag="PLI76_07185"
     CDS             1627..2940
                     /locus_tag="PLI76_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HOI61852.1"
                     /translation="MRNEEPGSPATGMKLQRELGLPAVTLSGIGIILGAGIYALLGEA
                     AGMAGNAVWLTFAIAAAIAAFSALSYAELSSIFPRAGAEFEYVSHAFGRRLAFVVGWL
                     IILSGILGAATVSLGFAGYFSELVGLPVIPSAILITLVLAGILFIGVRETARAAIVMT
                     LIEAGGIVMVILIGLPYLGRVDYFAMPQGIPGLLQASALVFFAYMGFEEIVKLSEETK
                     NPERTIPLALMIALAVSILLYILVSLAAVSVVGWEQLAASRAPFADVAAVALGPAAFT
                     LISVIALFATANTALLMMMASSRIIYGMASSYSLPPLLARVHPRTRTPWTAVVAVLLA
                     TIAFLFAGRIDFVANVTNFTLFVTFIVINASVIALRYRAPDMIRPFKVPGAVGRLPVI
                     PVVGMASSLFLLAQLGTPVLLVGGVMVVLGAGLALAGGWQPRGTA"
     gene            complement(3068..4588)
                     /locus_tag="PLI76_07190"
     CDS             complement(3068..4588)
                     /locus_tag="PLI76_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOI61853.1"
                     /translation="MTKYSFIARMPDEPGALHRAADIVKSYSGNINRIQYDRRIDPAT
                     VFFEVTATPETYVQMKEDLHAIGYLQETLPALGFLKFSLYLPHQPGSLFELLTYITGA
                     GANIAYIDFDDRRCDPGRVTISLNIEKTAIVESLLDRLKSRYRLDIIEYDMTGEKLDD
                     TVFYVRFAQAVRGLVGTAEDGFLLSFLHDVNHIVQELNSLGQDPKEVFESILATGRTL
                     RNTTGDRFYADLQRIRLSDAVEVFCFQMPCGGNIFLLRAPDETVMIDTGYGIYHEDVM
                     RMFRHYGLPNQQGIGRVYITHADADHCGAGGFSGEKVHTHAGSLAIIRQANRAYGSRS
                     ESSILEEVYTTVINLFSRFTPPENPDLFPAEMIGMRSIFPVLARVKVHDIEFEVLESL
                     GGHLHGQVYLFCPGHGIVFTADTLINFGSLDEDRKRYNSLADFLVTSVNVDSECARQE
                     RKALLELIRDLDEELSPHGRRCLVAGGHGAVSILNDGRLEAVGPIERYRAGGPKAD"
     gene            complement(4718..5224)
                     /locus_tag="PLI76_07195"
     CDS             complement(4718..5224)
                     /locus_tag="PLI76_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOI61854.1"
                     /translation="MADRILVAYATRYGSTADVAEAIGDELRKAGETVDVRPFTDIAD
                     LSPYRAAVIGSPIYMGRWLPESQVFIEKNQQFLRTIPVAYFAVGLTVKEGTHEFIRKA
                     EASMDQVRLLVQPVEVGVFPGKLETGRLTIADRAIVKLIRAEAGDFRDLAAVRAWAQA
                     VREKIAQA"
     gene            5459..6100
                     /locus_tag="PLI76_07200"
     CDS             5459..6100
                     /locus_tag="PLI76_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOI61855.1"
                     /translation="MAIRDRNARAWYHRGQTLCNGKKFDEAVACYDRALELSPDDPIL
                     WRRRGFTLVRTGRYHEAAASFDRALALAPDDATAWQRMGYALACLGQEKDAAACFEAA
                     LMRDPEHILAWQSRGWLLGIMCRYDEATACYEAVIRLDPSRGSAVWHRERMQERRDLA
                     ALADEVREAEQSIDVPACISDVVRERDYGNVALARAVLRELVGRAERSAVQRR"
     gene            complement(6088..6471)
                     /locus_tag="PLI76_07205"
     CDS             complement(6088..6471)
                     /locus_tag="PLI76_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOI61856.1"
                     /translation="MTALSGAAGRPDGRPDVREPDQERGRALTGISGLHLLHLTPVET
                     VVADDPSKPARELLATISDRIRNPLQVLLARADLMDDKETAGRIREQVRRINDAVKYL
                     EGALSDPYSTGKKGGPALDEDIHRR"
     gene            complement(6468..6863)
                     /locus_tag="PLI76_07210"
     CDS             complement(6468..6863)
                     /locus_tag="PLI76_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61857.1"
                     /translation="MVAKKLMGTCATAMLVVIAAACGHTGTGGESQPAGQPQEDVGAP
                     RSPGSAPERREQPQMDYASAAATFGVAGEEIRAAPGEPGQGRKTVADAAATLGVPEEA
                     FRVSEGTTGSGAPPGVSVGGELRERAGVP"
     gene            complement(6935..8149)
                     /locus_tag="PLI76_07215"
     CDS             complement(6935..8149)
                     /locus_tag="PLI76_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI61858.1"
                     /translation="MIGNDIIFALSVRSVRLHFLRSVLAALGIVIGVVAIASIGMMGA
                     NMTLSVTEQLSDMANKLTVTPYRGGGGGMMGGFGGGGGSSDDDAITEKEFKTIQRIVT
                     KYGTAYTVRSESDKIEVGSDEGRATIYGLDTETMREILTVESGEYPKSTTSVVVGPTL
                     AERLDLKIGSKIKIGDPDKGSTTTVRVVGILEERGMSMDLSTDMAIIGSDKLFVGIYG
                     GEGEYDQVNVVVSDLTDIETVKTAIENELNRKEDTVTVQDSSRMMESITSTVSTMTTF
                     VMAIAGISLLVAAVSIFNVMMMSVNERVREIGILRSIGTQRAEILRMFIYEAAIIGIV
                     GAIIGAIVSIVIGYVIVVGMIGSAEYFFAPGSIVYVPMAMTVGAGICIATGIYPAWRA
                     SNLDPIEALQNE"
     gene            complement(8146..8817)
                     /locus_tag="PLI76_07220"
     CDS             complement(8146..8817)
                     /locus_tag="PLI76_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI61859.1"
                     /translation="MSSPVIELVDVVRVYPLPSGDVTALSGVSLRIDEGEFVAIMGPS
                     GSGKSTLLNQIGCLDRPTSGDLSLAGKNTREMDDRELTNLRLSSIGYIFQKFNLIPLL
                     SAYENVEYPYIMKHRKNDDTGRVRDLLAMMGIDENLSAHKPNELSGGQQQRVAIARAL
                     VNDPAILLCDEPTGNLDSQTSLQIMEILADLHRRGRTLVMVTHNPETATYADRIITIR
                     DGQIA"
     gene            complement(8834..>10042)
                     /locus_tag="PLI76_07225"
     CDS             complement(8834..>10042)
                     /locus_tag="PLI76_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HOI61860.1"
                     /translation="LLSAIVGGATAADASAEEQIAVTSVSVNPDTLMRGDTGTVTVEI
                     KNTGETGVAISRAELYPNGISVLNDKTYNSVGTIGPGNTMSFTFTVRADAADGIYYPT
                     FYLDIRNGGSVRYSVPVTVESTEIQVNVVDAPETFPVDGKDTITLSVGNPREGSVNGI
                     TITPSGEGVRNTRTAVFLGALASDEEKNASFEITASRSTELTFDVSYRNGINEHHTIL
                     TVPIEIGERAVAPDMVVNNIEIARGGSAITLTGDVTNAGLKDAYSVKVTVDDPATPTD
                     PYPVYVVGGLEPDDFSSFEVTCNAEGASSIPLVVEYRDQDGRTFTETVTVSLNSASQA
                     SQAGAGSTMPSGMTGGPQGGRGGMGMSFGGGFSQIPVMEILLVVIGGVGVAVVWRKGY
                     LGKLRDRFRK"
BASE COUNT         1660 a         3191 c         3114 g         2077 t
ORIGIN      
        1 cgtgccgccg ccaccttctc ccgtggaaag acctcgccga gctcaaagag ggtgtttatg
       61 tcgatgtagc gtctcgcctg ctcgaagata tcctcttcct cgagggtaaa gtgcgcctcg
      121 atctcttccc ggaggccggc gagcgccggc agccaggcgt catcccggag agggatcacc
      181 tcgaaccggg cgagcgtctc gcggatggca gtatgatccg ccatcagccg cctgaccacc
      241 tcgggctttg cgccccgaag tcgtgcatac agggtggcct cctccgcatc catgtgcccg
      301 ggcagctcgc ggcggagcgt gatgcaccgg atgtcccgcg tatcagggtt gctctcgagt
      361 tcgtcgaaga gattccggat gaagtcgtgg tcgtcccgga tcagttcaag aatctggacc
      421 atattctgcc tccgtcggga gaggaataga ggatgagaga cttaacggtt tcgcccggcc
      481 aaaaaaagtg tgtacgggtg tccctgcact cctccttctg gtggttcacc gcggcgcaac
      541 cggaagcacg gccttctttg cgtcttcgta ccgggtgttc agggtcgaga gttcatcgct
      601 gctcatcttc ttctgcattg caggaaagag ctgatcctcc tcttcgcgaa tgtggtgggc
      661 cacgttctcc tggatcaccc tcatctttgc cacccagacg tcctcctctg cggtggatgc
      721 gctatcgagc tggctgagca gtgtcttgac tgcaccgtgc tcctcgatcc cctcaaggct
      781 tatatcccgc atctccggtt cttctctggt ccgaggatac aggacttttt cttccccgac
      841 gaggtgcggc agaaggctct ccttcatggt ggaatatttc tgctcccggt tgctcgtgcc
      901 cttctctgag agctcagcaa gctctgagag caccatcttg tgctcctctt tcagggtatc
      961 gataatactc tgcgccattc gatcacttcc cccccattcc cgggggggcc atctggtgcg
     1021 ggataggtat ataaattata tcccgggccg ggactacagg atcacctgag cggccgcagg
     1081 tgggccttcc ccttcgcctc ctcaaaccgg cccccaatat gcaggaggag ggcgtcatcc
     1141 atcgtccgtc gggcctccga aaagaccatc ttctcctccc gctcgatatg gttcctcatc
     1201 gcctcgcgga gctccggcac cacgcggacc cagtcatcct cgtcggccat ggggatgccg
     1261 ttcagccgat cgacgatctc ccgggcggcc ttatgctccc cgcgagagac cgccatgaga
     1321 tcggataacc cccgtgcctc aagctcgggg taaaaaactc tctcctcgcc ggccatgtgc
     1381 ggcaggagtt cgtttttcat ccggttgaac ttatccgccc ggtcgctgat gccttttctc
     1441 tgcaactcgt ccagtccggc aagcaccttt gcatgttccc gccgtaagag gtccaggatg
     1501 ttctgcccca tcgttctccc ccacgctgag tgctccccgg tgtcgggaac ggataaatac
     1561 ctatgctccg cctccggggg tcgcctgccg gatacccata aatggagcag atgcgtatga
     1621 tctcacatgc gaaacgagga accgggctct cctgcgaccg ggatgaagct tcagcgcgaa
     1681 ctcggtctcc ctgcggtcac cctctcgggc atcgggatca tcctcggcgc cgggatatac
     1741 gcccttctcg gggaagcggc cggcatggcc ggcaacgccg tctggctgac gtttgccatc
     1801 gccgccgcca tagccgcgtt cagcgctctc agctacgccg aactctcgtc gatctttccc
     1861 cgggccggcg ccgagttcga gtacgtctca cacgcatttg gaaggaggct tgcgttcgtc
     1921 gtcgggtggc tgatcatcct ctccggcatc ctcggcgccg cgaccgtctc gctcgggttc
     1981 gccggctact tttcagagct cgtcggtctg cccgtcatcc cctccgccat cctgatcacc
     2041 ctcgtccttg ccggcatcct ctttatcggg gtcagggaga ccgcccgggc agccatcgtc
     2101 atgaccctca tcgaggccgg gggcatcgtc atggtcatcc tgatcggcct cccgtacctg
     2161 gggcgggtgg actactttgc tatgccccag ggcatcccgg gcctcctgca ggcatcggca
     2221 ctcgtcttct ttgcctacat ggggtttgag gagatcgtaa aactctcgga ggagacgaaa
     2281 aatcccgagc ggaccatacc tctcgccctc atgatcgcgc ttgccgtctc catcctcctc
     2341 tacatcctgg tctccctcgc cgccgtctcg gtcgtcggat gggagcagct cgccgcctcc
     2401 cgggcaccct tcgccgacgt cgcggcggtc gccctcggcc ctgcggcgtt caccctcata
     2461 tccgtcatcg ccctctttgc cacggctaac accgccctcc tcatgatgat ggcctcgtcg
     2521 cggatcatct acgggatggc gtcctcgtac tcgcttcccc ccctcctcgc ccgggtgcac
     2581 ccccggacac gaacaccctg gacggccgtc gtcgccgtcc tgctcgccac catagccttc
     2641 ctctttgcgg gcaggatcga cttcgtcgcg aacgtcacga actttacgct ctttgtgacg
     2701 ttcatcgtca tcaacgcatc cgtgatcgcg ctccggtacc gtgcgcccga catgatccgg
     2761 ccattcaagg ttcctggcgc cgtcgggaga ctgccggtca taccggtcgt cggtatggca
     2821 tcgagcctct tcctccttgc ccagctcggc accccggtcc tgctggtcgg cggggtcatg
     2881 gtcgttctcg gtgcaggtct cgcactggca ggagggtggc agccccgcgg gacagcgtag
     2941 agaggagtga acggcatcgg ggtttgagag acgggtacct gaaacttcct tcgcactcaa
     3001 cgcgcaagac cgtatcgcca tcggcaatgg gcagctcgcg tgctacagca tgatacggcg
     3061 cggttcgcta atcggccttt ggtcccccgg cccggtaacg ctcgatcgga ccgaccgctt
     3121 ccagtctccc gtcgttgagg atggagacgg cgccgtgacc gcccgcaacc aggcaccggc
     3181 ggccgtgcgg cgagagttcc tcatccagat cccggatcag ctcaagaagc gccttacgtt
     3241 cctggcgtgc acactcgctg tcgacgttga ccgacgtcac caggaagtcc gcaagcgagt
     3301 tgtagcgctt ccggtcctca tcgaggctcc cgaagttgat cagcgtatcg gcggtgaaga
     3361 cgatcccgtg ccccgggcag aagaggtaga cctggccgtg caggtgcccg cccagggact
     3421 cgaggacctc gaactcgatg tcgtggacct tcacgcgggc gaggacgggg aagatcgagc
     3481 gcatgccgat catctccgcc gggaagaggt cggggttctc aggcggtgtg aagcgcgaga
     3541 agaggttgat gacggtcgtg tagacctcct cgaggatcga ggactcgctg cgcgaaccgt
     3601 aggcccggtt cgcctggcgg atgattgcaa gcgacccggc atgggtatgg actttttcgc
     3661 ctgagaaccc tcccgccccg cagtggtccg cgtcggcgtg ggtgatgtag accctgccga
     3721 ttccctgctg gttcgggagg ccgtaatgcc ggaacatccg catgacgtcc tcgtggtaga
     3781 tcccgtatcc cgtgtcgatc atgacggtct catccggggc gcggaggagg aagatgtttc
     3841 caccgcacgg catctggaag cagaagacct cgacggcatc gctgagccgg atacgctgga
     3901 gatccgcgta gaaccggtcc ccggtggtat tccgaagcgt ccttcccgtg gcgaggatgg
     3961 actcgaagac ctcttttggg tcctggccga ggctgttgag ttcctggacg atatggttga
     4021 cgtcatgcag aaaagagagc aaaaaaccat cctccgccgt cccgaccagc ccccgcaccg
     4081 cctgggcgaa cctgacgtag aagacggtgt cgtcgagttt ctcgccggtc atgtcgtact
     4141 cgatgatatc gagacggtag cgcgacttga gacggtcgag caggctctcc acgatcgcgg
     4201 tcttctcgat gttcaggctg atggtgaccc tcccggggtc gcaccgccgg tcgtcgaagt
     4261 cgatgtaggc gatgtttgcc cccgcaccgg tgatgtaggt gaggagctcg aagagggagc
     4321 cgggctggtg gggcaggtag agggagaact tgaggaaccc gagcgccgga agcgtctcct
     4381 ggagatagcc gatggcgtgg aggtcttctt tcatctgtac gtaggtctcc ggcgtcgcgg
     4441 tcacctcaaa gaagaccgtg gcaggatcga tccgccggtc gtactggatg cggttgatgt
     4501 tccccgagta agacttgacg atatcggcgg cacggtggag cgcgcccggc tcgtcgggca
     4561 ttctggcgat gaaggagtac ttggtcaccc ggttcacctg gagatatcgg tactatgctc
     4621 tcgtcctata tccttcttcc ggcaaaaatg gtcctgatcg ggggataggg cataccggga
     4681 gccccgttcc gggtgtggac atagggaggg cgaccggtca cgcttgcgcg atcttctcgc
     4741 gcaccgcctg cgcccaggca cggacggccg cgagatcccg gaagtcccct gcctctgccc
     4801 ggataagttt gacgatcgcg cgatccgcga tcgtaaggcg gccggtctca agcttcccgg
     4861 ggaagacgcc gacctcgacg ggttgtacaa gcagccggac ctggtccatc gaggcctccg
     4921 ccttccggat gaactcgtgc gtcccctctt tgaccgtgag ccccacggcg aagtaggcga
     4981 ccgggatggt gcggaggaac tgctggttct tctcgatgaa gacctgcgac tccgggagcc
     5041 atctccccat gtatatcggg ctcccgatga cggccgcacg gtagggggag aggtcggcga
     5101 tatcggtgaa cggccggacg tcgaccgtct cgcctgcctt ccggagttca tcgccgatcg
     5161 cctccgccac atccgcggtc gaaccgtagc gggtggcgta ggcaacaaga atcctgtctg
     5221 ccatgatgta cctatctctc atcttgatgg ccataaacgt tatcgggagg ccgggagcca
     5281 acggcatcgg gtcgtcgtat cccgaggcgc cggcgggggc cgggagcgcc ggtctcctgc
     5341 cgacttctgc gcgaggcggc ggtgcgggga cggcgtgcgg ctgctcgcgt gaaggaggcg
     5401 aggaataccc tgtcgcggtc catcaggttc ttatggattg gccgccctca taggtccaat
     5461 ggcgatcaga gacaggaatg ccagagcatg gtaccacagg gggcagactc tctgcaacgg
     5521 caaaaagttc gatgaagcgg tcgcctgcta cgacagggcc cttgaactct cacccgatga
     5581 ccctatcctc tggaggcgca gggggtttac cctcgtcagg accggccgct atcacgaggc
     5641 ggccgccagt tttgaccggg cgcttgctct tgctcccgac gatgcgacgg cctggcagcg
     5701 gatggggtat gcgcttgcct gcctgggcca ggaaaaagat gccgccgcct gctttgaggc
     5761 ggcacttatg cgggacccgg agcacatcct cgcctggcag agccggggct ggctgctcgg
     5821 gatcatgtgc cgctacgacg aggcgacggc ctgctacgag gcggtcatca ggctcgaccc
     5881 ctcccgcggg tctgcggtct ggcaccggga gcgtatgcag gagaggaggg accttgcggc
     5941 gctcgcagac gaggtccggg aggcggagca gtccatcgac gtgccggcct gcatctcgga
     6001 cgtcgtccgc gagcgggact acggcaacgt cgcccttgcg cgggcggtgc tccgcgaact
     6061 ggtcgggcgg gcggagaggt cggcggttca gcgccggtga atgtcctcgt ccagtgccgg
     6121 tccccccttc tttccggtgc tgtacggatc ggataaggcg ccctcgaggt atttgacggc
     6181 atcgttgatc cggcggacct gctctcgaat ccttccggcg gtctctttat cgtccatcag
     6241 gtccgcccgg gccaggagca cctgcagggg gtttctgatg cgatcggaga tggtggcgag
     6301 gagttcccgg gcaggttttg acggatcatc ggcaacgacc gtctctaccg gcgttaaatg
     6361 caggaggtgc agcccggaga tcccggtgag ggcccgaccg cgttcctgat cgggctcgcg
     6421 aacatccggt cttccgtccg gcctccccgc ggcaccgctg agcgctgtca tggaactcct
     6481 gcacgctccc gaagttctcc ccctacggag actccggggg gagcgccgct ccctgtcgtt
     6541 ccttcggaaa ctctgaacgc ctcttccggg acaccgagcg ttgccgccgc atcggcaacg
     6601 gtcttccggc cctggcccgg ttcaccgggt gccgcccgga tctcctcccc ggcgaccccg
     6661 aacgtggccg cggctgaggc gtagtccatc tgcggctgct ctcgccgttc gggggccgat
     6721 cctggcgaac ggggcgcccc cacgtcttcc tgcggctgcc ctgcaggctg cgattcaccg
     6781 cccgtgccgg tgtgcccgca cgctgctgca ataacgacga gcatggcggt tgcacaggtg
     6841 cccatcagtt tctttgctac catacttctc tcttccaatc tgttctcttg cgacggatgc
     6901 ccggggtaaa aacaggtgtt cccctgtttg tacgctattc gttctgcaac gcctcgatcg
     6961 ggtcgaggtt tgaggcccgc cacgccgggt agatgcccgt cgcgatgcag attccggcgc
     7021 cgacggtcat ggccatcggg acgtagacga tgctcccggg agcgaagaag tactcggcgg
     7081 agccgatcat gccgaccacg atgacgtaac cgatgacgat gcttacaatg gcaccgatga
     7141 ttgctccgac gatcccgatg atcgccgcct cgtagatgaa catccgcagg atctctgccc
     7201 gttgcgtccc gatggagcgg aggatcccga tctcacgtac ccgctcgttc accgacatca
     7261 tcatgacgtt gaagatcgag actgcggcaa cgagaagcga gatacccgcg atcgccatca
     7321 cgaacgtcgt catcgtcgag accgtcgagg tgatgctctc catcatgcgg ctgctgtcct
     7381 ggacggtgac ggtatcttct ttccggttga gttcgttctc gatcgccgtt ttgaccgtct
     7441 cgatgtcggt taagtcgctc acgacgacgt tgacctgatc gtactcgccc tcgccgccgt
     7501 agatcccgac aaagagtttg tcgctgccga tgatcgccat atccgtggag aggtccatcg
     7561 acatgccgcg ctcttcgagg ataccgacga cccggacggt cgtggtggac cccttgtcgg
     7621 gatcgccgat cttgatcttg ctccctatct tgaggtcgag tcgctcggca agcgtcggcc
     7681 cgaccaccac cgaggtcgtg ctcttgggat actctcccga ctcgacggtc aagatctcgc
     7741 gcatggtctc cgtatcaaga ccgtagatag ttgctctccc ttcgtccgat ccgacctcga
     7801 tcttatcgga ttcagacctg acggtgtacg cggtcccgta cttcgtgacg atgcgctgaa
     7861 tagtcttgaa ctctttttcc gtgatcgcat cgtcgtcgga gctgccgcct ccaccaccga
     7921 accctcccat catgccgccc ccgccaccgc ggtatggggt gacggtgagt ttgtttgcca
     7981 tgtcggagag ctgttccgtg accgagagcg tcatgttcgc tcccatcatc ccgatcgagg
     8041 cgatggcgac gacgccgatg acgatgccga gggcggcaag gaccgatctg aggaagtgga
     8101 gccggacgct ccgtacggag agagcgaaga tgatgtcgtt tccaatcatg cgatctgccc
     8161 gtcccgtatc gtgattatcc tgtccgcgta cgtggcggtc tcggggttgt gggtcaccat
     8221 gacgagcgtc cggcccctgc ggtgaaggtc tgcaaggatc tccatgatct ggaggctcgt
     8281 ctgcgagtcc aggtttccgg tcggttcgtc gcagagaagg atcgccggat cgttgacgag
     8341 cgccctggcg atcgcgaccc gttgctgctg gccgccggag agttcgtttg gcttatgtgc
     8401 cgagaggttt tcatcgatcc ccatcatggc gaggagatcc cgcacccttc cggtgtcatc
     8461 gttctttctg tgcttcatga tgtagggata ctcgacgttt tcgtaggcag agaggagggg
     8521 gatgaggttg aacttctgga agatgtagcc gatggaagag agtcggagat tggtcagttc
     8581 ccggtcgtcc atctcccggg tgttcttccc cgcgagagag aggtcgcccg acgtaggacg
     8641 gtcgaggcac ccgatctggt tcaagagggt cgattttccc gaccctgacg gccccatgat
     8701 agcgacgaat tctccctcgt cgatcctgag ggataccccc gagagggccg tgacgtcgcc
     8761 tgaggggagg gggtaaaccc tgacgacatc tacgagttcg ataaccggac tgctcatctg
     8821 tcttcccccg ggatcacttg cgaaaccggt cgcggagctt tccgaggtat ccctttcgcc
     8881 acacgacggc gacgcccacc ccgccgatga cgacgaggag gatctccatc accgggatct
     8941 gcgagaaccc gccgccgaac gacatgccca tgccccctct tccaccctgc gggccgccgg
     9001 tcatgccgga cggcatcgtg ctgccggcgc cggcctgcga cgcctggctt gcggagttga
     9061 gcgagacggt cacggtctcc gtgaaggttc tgccgtcctg gtcacggtat tcgacgacga
     9121 gcgggatgga ggatgcacct tctgcgttgc aggtgacttc gaaactcgag aagtcgtccg
     9181 gctcgagccc gccgacgacg tagaccgggt aggggtcagt cggggttgcg ggatcgtcga
     9241 cggtgacctt gaccgagtat gcatctttga ggccggcgtt ggtcacgtcc ccggtcagcg
     9301 tgatcgcgct gccgccccgg gcgatctcga tattgttgac caccatgtcg ggcgcaaccg
     9361 cccgctcacc gatctcgatg gggacggtca ggatggtgtg gtgctcgttg atcccgttcc
     9421 ggtaggagac gtcgaaggtg agctcggtcg accgcgacgc cgtgatctcg aacgaggcgt
     9481 ttttttcctc atcagacgcg agagcgccga ggaagacggc cgtccgggtg ttcctgacgc
     9541 cttcgccgct cggggtgatg gttatgccgt tcacgctgcc ctcacggggg ttcccgacgg
     9601 agagcgtgat ggtatctttg ccgtccaccg gaaaggtttc cggagcgtcg acgacgttga
     9661 cctggatctc cgtgctctcg accgtcaccg gaacggagta gcggacgctt cccccgttcc
     9721 ggatgtccag gtagaaggtc gggtagtaga tgccgtccgc ggcatcggcc cgcacggtga
     9781 aggtgaacga catcgtgttc cccgggccga tggtgcccac tgaattgtac gtcttgtcgt
     9841 tgagaaccga gatcccgttg ggatagagtt ccgccctgct gatggcaacg cctgtctccc
     9901 cggtgttctt gatctcgacc gtcaccgtgc cggtatcccc gcgcatgagc gtgtcggggt
     9961 tgacgctgac actggtgact gcaatctgtt cctccgccga cgcatcggcc gccgtggcac
    10021 cacccacgat tgccgagagg ag
//
LOCUS       DAOSEE010000081         9827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_2601_length_9827_cov_223.629144, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000081 DAOSEE010000000
VERSION     DAOSEE010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 9827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9827
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2601_length_9827_cov_223.629144"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            391..1593
                     /locus_tag="PLI76_07230"
     CDS             391..1593
                     /locus_tag="PLI76_07230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOI61861.1"
                     /translation="MKVALLCEYSRDMGAYQGSNIYITELITRLAGRDDIDLHLITMG
                     RENAVRQEEGFTHHILRKGGPLSLPYFHPLVLVRMVKTVRAIRPDIVHALSTRYPCST
                     AAVLLQNDYPSLVTAFGIFEREIAHYREDMLPLERTLSHIFHTFFIRNERWVLSRAPE
                     LVVDAPSIGDLVRTPTTGRIHVVAAGLDLERLRPQILSPPAGEGPDIVFINTLTRLKG
                     ADILLSALPGVVEAYPGIRACIGGKGPQAPELRRLARDLGLEANVIFPGFVPDEEKYR
                     YYRQCRMVVVPSRWDCQPSPLFEAAAFGKPVVASDMAHPGIVEDGVTGLIFRSDDAAG
                     LEEKILFLLDDDAARERMGRAAAARVGEYDWPRVVDRYVTIYQDAVREHRRGGKRATA
                     PAGASPER"
     gene            1606..2727
                     /locus_tag="PLI76_07235"
     CDS             1606..2727
                     /locus_tag="PLI76_07235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOI61862.1"
                     /translation="MRESTIGLLTFPIERSGIHPTSNLIGILSSLTREPIPLVTGNAG
                     RAFENDARVRFSGVDHAGGGSVASRVLQNIATQVRMARRIREVDGTGTWLFFFGGEVL
                     VLPMLAAKLSGKKVILALPGHTPLMMAPARDRLLLPAGILTGITCRLCDRIVLYSPAL
                     VDHWGLGQYREKITIAREHLLDFEAFRVKVPLDERPMAVGYIGRLADEKGVWNFVRAL
                     PRLAADTDLHFLIGGDGPLLPAIREFIRESGLSERVTLAGWIRHDDLPCHLNRLRLLV
                     MPSCTEGLPNIMLEAMACGTPLLAAPVGAVPDFVHDGVTGFILAPNTPEGIAASVIDI
                     LGRSELTSIAETARKCVEQEITYERAVERFAAVIEGLGD"
     gene            2733..4604
                     /locus_tag="PLI76_07240"
     CDS             2733..4604
                     /locus_tag="PLI76_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61863.1"
                     /translation="MHLDKILAGTGIVVSAALVAYIALTTGRLIFILAGVLTLICCLL
                     WLAVRERAIREFPVSWAGGAETGNGRSSTVLLILFFALYVLSIAALHTRASLYERPTG
                     YFVLSSLMAGVIALEILLPHRRKARRVLPQIVLLGVSVVWSQILIVPGILGSDPWWHR
                     MFVSQILDLSAIPGGYWYTHMPLFHLEVAGASLLAGLDYRLAAMLSVSLAQIIILPVV
                     VYLLGNALFEDERVGLLAGLLLITANQQIGMSTASIPNAFAAIFIALVLFLLFRLKYT
                     RPVRAGYLAYLLLAAIILTHTIAAACMALILFVVWGACRVHGRIRDDAAAKPYPVTLG
                     LASLFLAAMLGWWYFVSGHIAVLADLIRQGFTRAYFIQNPAVVDAYPGLVPVPEQVFN
                     NIGMFAFFTVSLVGIFYLAARRERNGFAFGMVGIILLGIGFFPLITGLSFIEHRWWYF
                     SQILLAVPLAASLLLVAGREKAHRMPAALTLAACVGLLAFTMIASPAADTGIPLFSPN
                     SAVRAGFTGSELQAIDTVGGIRAGAIGADRYVQVVNYVNPGPGFVVPADDMLIEGAYR
                     SHGMDAVLVREYIRGHPIFCYDGMFRLTHDPAMTLAREGYYRVYESGSVGLFRLDGL"
     gene            4673..5176
                     /gene="fae"
                     /locus_tag="PLI76_07245"
     CDS             4673..5176
                     /gene="fae"
                     /locus_tag="PLI76_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HOI61864.1"
                     /translation="MVSFEHLLIGEALVGEGPEVAHIDLVIGKKGSSVEQAFVTALAS
                     PARGHTPLLAVLTPNIPAKPATLMVNKVTIRNAGQALLIFGPAQAAVAKAVVDSVAEG
                     IIPGAEADNLLIIASVFIEWDAADKKKIYDWNYEATKLAIRRAVAGEPKMQEVLARKD
                     TAKHPFA"
     gene            complement(5189..5932)
                     /locus_tag="PLI76_07250"
     CDS             complement(5189..5932)
                     /locus_tag="PLI76_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61865.1"
                     /translation="MIPLFPLPAAAVLAAVHLVAGRLRFLDVVPRSRWLSLAGGVSVA
                     YVFVRLLPELSAGQEVVQRAIAGELLAGLENHIYIVALLGLAIFYGLERVVRRSQEER
                     STTQDIERTTTGPGVFWLHIASFGVYNILIGYLLLHRIRPGLMSLAFYTVAMALHFLV
                     TDYGLRNDHRRDYDRVGRWALSIALAGGLAAGYASRLPELWIAVITAFLAGGVILNVL
                     KEELPEERKSRIAPFFIGAFGYAAILLLA"
     gene            complement(5965..7362)
                     /locus_tag="PLI76_07255"
     CDS             complement(5965..7362)
                     /locus_tag="PLI76_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOI61866.1"
                     /translation="MGLQRGLSQFDLTNIIVGAIVGADIYIASALTAGLVGPFSIVLW
                     AVAGLFAATIAMVFAYCSYYVPRVGGPFAYVSEAFDDFYGFLTGWSMWIAELLALPVF
                     AIAFTNYLQALVPLTTAAEIAVRALFVASLTLVNIVGVRAAGRLNDLLTLIKLAPLLL
                     LIVAGLGIFLIRPEVFIGNYTPLLPFGLDNASHALVLVFWAYAGFELGTLPAGEVENP
                     QTAIPRAITTGMLIVAVFYLLTNFVLFGLVNWTLLDRSAVPLVVAGTALFGSAGAIIM
                     TVGALFSVSGSNESGMLGSARLAYAMAIDGLFPRAFARLHPRYDTPHVVLIAQGVIAF
                     ILSNIGNLSNLISFAVLTLAFSFLLTCFALIVLRDDNAAHLRGQHILPLAGIGVCLFL
                     LFSTTMLDKVAGVLVILAGIPIYLFYSPKQDIRHLKALFLSEEAVLRRRLEAKETYLA
                     NLLDIIRTVSRKIGR"
     gene            7486..7656
                     /locus_tag="PLI76_07260"
     CDS             7486..7656
                     /locus_tag="PLI76_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOI61867.1"
                     /translation="MRFRIVVREDPEDGGYVVSCPAIPGCHSQGETLEEALENISDAI
                     SGCVEVLNERAE"
     gene            complement(7738..7809)
                     /locus_tag="PLI76_07265"
     tRNA            complement(7738..7809)
                     /locus_tag="PLI76_07265"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7774..7776),aa:Gly,seq:gcc)
     gene            complement(7963..8658)
                     /locus_tag="PLI76_07270"
     CDS             complement(7963..8658)
                     /locus_tag="PLI76_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HOI61868.1"
                     /translation="MVAIEPLVLLVLALGPGVFWLWYFYHRDKYEPEPAALIVKVYLL
                     GVLVTFPVAFVEGFVGLLIASPLIMGVIIAPIVEEYGKFTVVYRFVYRDAEFDEPMDG
                     IVYAAAAALGLASLENVLYVLTAYVTSPTLAFETLAIRAIFSVPAHALFSSVWGYALG
                     RAKFTAAERRPGIIFRGLALAMMLHGTFNLLLFLSDIVAYATVIFVLVLIPGLWILAN
                     RNIRIALRWQRRR"
     gene            complement(8719..9666)
                     /locus_tag="PLI76_07275"
     CDS             complement(8719..9666)
                     /locus_tag="PLI76_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61869.1"
                     /translation="MAEEIYAFNRLEGAFHRMKSLLWPIKWGIWLRLAVIALFVGGGI
                     SIPNVFQYQFEEGDFLAGNAGSFPEILPMVVLGIIAVVLAIALVWMLIGATMQFVFVD
                     MLSTGDIHIRRFFRERLGKGTRLFLFELGITVAMVLAIMALFLILFGFGSIGTGAAAA
                     LLIIALIPIILVAALVFGLIFLLTTDFVVPIMIREDCGVIEGWQRLIGVISAGFWQTA
                     AYVVVRFVVGLIAAIVQAVLVIIALLIIAIPFVLIGVVLLAAFQGGNIALFVVLLIPY
                     LIIAIPAALLIAVPFVTFFRYYALLVLEGLGPGYRLLPE"
BASE COUNT         1883 a         3084 c         3147 g         1713 t
ORIGIN      
        1 gtgcccggag atcggcgttg tgcgccggcc ctcgtggcga ggcccggtcc ccgttcttct
       61 ccggcctatc cccacggggt ggaccgtggc gtctctcgcc acgggggggg ccggccatgc
      121 ccggcatcta tccggacggc tgcccgccct tctcccccct ctccggccta tttccgtata
      181 tttcgggtga agtgacggct cccggcgccg gctgcccgcc cggtctctct ggcggcaacg
      241 tttcggcagc ctgcatgtgg gccggtgcgg tttgcggtgc agggctcctc aaactatata
      301 tgggttgatg gtgattgcat ccctgcgcca catgactgga ccgttgaaga accctatccc
      361 ggcggggcag cacagtccgg gggagatatc gtgaaagtag cccttctctg cgagtactca
      421 cgggacatgg gggcatacca gggttcaaac atctacataa cggagttgat aacacgcctc
      481 gccgggcgtg acgatatcga tctccatctc atcaccatgg gcagggagaa cgccgtacgg
      541 caggaagagg ggttcaccca ccatatcctc cggaagggcg gcccgctctc cctcccctac
      601 ttccaccccc tggtgctcgt acggatggtg aagaccgtca gggccatccg gccggatatc
      661 gtccacgccc tctccacccg gtacccctgc tccacggccg ccgtcctcct ccagaacgac
      721 tacccctccc tcgtgaccgc gttcggcata ttcgagagag agattgcgca ctaccgcgag
      781 gatatgctcc ccctcgaacg gacgctctcc catatcttcc acaccttctt catccggaac
      841 gagcggtggg tgctctcgcg ggcgcccgag ctcgtcgtgg atgccccctc gatcggggat
      901 ctcgtcagga cgccgacgac cggccggatc cacgtcgtcg ccgccggcct cgaccttgaa
      961 cggctccggc cgcagatcct ctcccccccg gccggagagg ggccggacat cgtcttcatc
     1021 aacacgctca cccggctgaa aggcgcggat atcctgctat ccgcccttcc gggcgttgta
     1081 gaggcgtatc ccggcatcag ggcgtgcatc gggggaaagg gtcctcaagc acccgaactc
     1141 cggagactcg cccgggatct cgggctggag gcgaacgtca tcttccccgg tttcgtgccc
     1201 gacgaggaga agtatcgcta ctaccggcag tgccggatgg tggtcgtccc ctcccgatgg
     1261 gactgccagc cgtcccccct cttcgaggcc gcggcgttcg gcaaacccgt cgtcgcatcg
     1321 gacatggctc atcccggcat cgtcgaggac ggggtgaccg ggctcatatt ccgctccgac
     1381 gacgcggccg ggcttgaaga gaagatcctc tttctcctcg acgacgatgc cgcacgcgag
     1441 cgcatgggcc gtgccgccgc ggcacgggtc ggcgagtacg actggccgag ggtcgtcgac
     1501 cggtacgtca cgatatacca ggacgccgtc agggagcacc ggcggggcgg gaagcgggct
     1561 acggcgcccg ccggtgcctc cccggagagg tgaggagaga ctgccatgag agagagtacc
     1621 atcggactgc tcacgttccc gatcgagcga tcgggcatcc acccgacatc taacctcatc
     1681 gggatcctct cgtcccttac acgcgagccg atccccctgg tgaccgggaa tgccgggcgc
     1741 gccttcgaga acgacgcgcg ggtccggttc tccggcgtcg atcacgcggg cgggggaagc
     1801 gtggcatcaa gagtcctgca gaacatagca acgcaggtga ggatggcccg ccgcatcagg
     1861 gaggttgacg gcaccggcac ctggctcttc ttcttcggcg gcgaggtcct cgtcctgccg
     1921 atgctcgcgg caaaactctc cggaaaaaag gtcatactgg cgctcccggg ccataccccc
     1981 ctcatgatgg cgccggcacg tgaccggctc ctcctcccgg cggggatact caccggtatc
     2041 acctgcaggc tctgcgatag gatcgtcctc tactccccgg ccctcgtaga ccactgggga
     2101 ctcggacaat accgggagaa gataacgatc gcccgcgaac acctcctcga cttcgaagcg
     2161 ttcagggtaa aggttccgct cgacgagcgg cccatggccg tcggctacat cggccgcctc
     2221 gccgacgaga aaggggtctg gaacttcgtc cgcgccctcc cgcgccttgc tgccgatacc
     2281 gacctgcact ttctcatcgg cggcgacgga cccctgctcc cggctatccg ggagtttatc
     2341 cgggagagcg gcctctccga acgggtgacc ctcgcgggat ggatacgcca cgacgacctc
     2401 ccctgccacc tcaaccgcct caggctgctc gtgatgccgt cctgcacgga agggctcccg
     2461 aacatcatgc tcgaggccat ggcctgcggc acgcccctgc tcgcggctcc cgtgggcgcc
     2521 gtgcccgact tcgttcatga cggcgtgacg gggtttatcc tggcgccaaa cacaccggag
     2581 ggcatcgcag cctcggtcat cgatatactc gggcggtcgg agctgacatc catcgcagaa
     2641 accgccagaa agtgcgtaga gcaggagatc acctacgaac gggcagtgga gcggtttgcc
     2701 gccgtcatcg aagggctcgg ggattgacgg caatgcacct cgataagatc ctcgccggta
     2761 ccggcatcgt cgtctcggcc gccctcgtcg cctacatcgc gctcaccacc gggcgcctga
     2821 tattcatcct cgccggcgtt ctcaccctca tctgctgtct cctctggctt gcagtccgcg
     2881 agcgggcgat ccgggagttc ccggtatcgt gggccggcgg ggcagagacc ggaaacggcc
     2941 gatccagcac cgtgctgctc atcctcttct tcgccctcta cgtgctgagc atcgccgcgc
     3001 tccatacccg cgcctcgctg tatgagcggc caaccggcta ctttgtcctc tcatcgctca
     3061 tggccggcgt catagccctc gagatcctcc tgccgcaccg gagaaaggcc cgccgggtcc
     3121 tgccgcagat cgttctcctc ggcgtatcgg tcgtatggtc gcagatcctt atcgtcccgg
     3181 gcatactcgg ctcggatccc tggtggcaca ggatgttcgt atcgcagatc ctggacctct
     3241 ccgccatacc gggaggttac tggtacaccc acatgccgct cttccacctg gaggttgccg
     3301 gggcctcgct ccttgccggg ctggactacc gcctcgcagc gatgctctcc gtgagcctgg
     3361 cacagatcat catcctcccg gtggtcgtct acctcctcgg gaatgccctc tttgaggacg
     3421 aacgggtcgg gctgctcgcc gggctcctgc tcatcaccgc aaaccagcag atcggcatga
     3481 gcactgcatc gatcccgaac gccttcgccg cgatattcat agcccttgtc ctctttctcc
     3541 tcttcaggct gaagtataca cggccggtgc gggccgggta tcttgcatac ctcctccttg
     3601 ccgccatcat cctgacgcac accatagcgg cggcgtgcat ggcgctgatc ctcttcgtgg
     3661 tgtggggcgc gtgccgggtc cacggccgca tccgggacga tgcggccgca aaaccgtatc
     3721 ccgtcacgct gggcctcgcg tcgctcttcc tggccgccat gctcgggtgg tggtacttcg
     3781 tctcagggca catagcggtc cttgccgacc tcatccgcca ggggttcacc cgggcctact
     3841 tcatccagaa cccggcggtc gtcgacgcgt atcccgggct cgtgccggtc ccggaacagg
     3901 tcttcaacaa catcggcatg ttcgccttct tcaccgtaag cctcgtcggc atcttctacc
     3961 ttgccgcacg gcgggagagg aacgggtttg ccttcggtat ggtggggatc atcctccttg
     4021 gcatcggctt cttcccgctc atcacgggcc tctcgttcat cgaacaccgg tggtggtact
     4081 tctcccagat cctccttgcc gtcccgctcg cggcgtcgct tctcctggtt gcagggagag
     4141 agaaggccca caggatgccc gccgcactca cccttgccgc gtgcgtcggc ctccttgcct
     4201 tcaccatgat cgcaagcccg gcagccgata ccggcatccc gctcttctca ccgaacagcg
     4261 ccgtacgggc cgggtttacg gggtcggaac tccaggcaat cgatacggtg ggcggcatac
     4321 gcgccggggc tatcggggcg gacaggtacg tccaggtggt aaattatgtc aacccgggcc
     4381 cggggttcgt cgtgccggcc gacgatatgc tgatcgaggg ggcgtatcgt tcgcacggga
     4441 tggatgccgt cctggtccgg gagtacatcc ggggtcaccc catcttctgc tacgacggca
     4501 tgttcaggct gacgcacgat ccggctatga cgcttgcccg ggagggctac taccgggtgt
     4561 acgaatccgg ctcggtcggc ctgttcaggc ttgacggcct gtagtgccgg cgcattctcc
     4621 acaacctttt tcttccccgc accccatccc acaacccggg aggtttacta ccatggttag
     4681 ttttgaacat ctactcatcg gagaagcgct cgtcggcgag gggccggagg ttgcccacat
     4741 cgatctcgtc atcgggaaga aggggagcag cgtggagcag gccttcgtca cggcgctcgc
     4801 atcgcctgcc cggggccaca cgccgctgct tgcggttctc accccgaaca tcccggcaaa
     4861 gcctgcgacg ctgatggtca acaaggtcac gatccggaac gccggccagg ccctcctgat
     4921 cttcggcccg gcacaggctg ccgtcgcgaa ggcggtggtg gatagcgttg cggaaggtat
     4981 cattcccggg gcggaggccg ataacctgct gatcatcgca tcggtcttca tcgagtggga
     5041 tgcggccgac aaaaagaaga tctacgactg gaactacgag gcgacgaagc ttgccatccg
     5101 ccgggcggta gcgggcgaac cgaagatgca ggaggtgctt gcacggaagg ataccgcgaa
     5161 gcatcctttt gcgtgattgc cgtcgctttc acgccagaag gaggatggcg gcatacccga
     5221 acgcgccgat gaagaaaggc gctatacggc tcttgcgctc ctcggggagc tcttccttga
     5281 gcacgttgag gatgacgccg ccggcgagga acgcggtgat gacggcgatc cagagctccg
     5341 ggagccggct tgcgtagcct gccgcaagac cgccggcgag cgctatggag agcgcccatc
     5401 gccctacccg gtcgtagtcc cggcgatgat cgttacgcag cccgtagtcg gtgaccagga
     5461 agtggagtgc catggcgacg gtatagaatg caaggctcat gaggccgggc cgtatacggt
     5521 ggagcaggag gtagcctata aggatgttgt agacgccgaa ggatgcgata tggagccaga
     5581 agactcccgg gccggtcgtc gtgcgttcga tatcctgcgt tgtcgaccgt tcctcctgcg
     5641 acctcctgac gacccgctcc agaccgtaga agatggcaag accgaggagc gcgacgatgt
     5701 agatgtggtt ctcaaggccg gcgagtagct ccccggcgat ggctctttgg acaacctcct
     5761 ggccggcgct caactcgggg agcaggcgga cgaagacgta ggcaacggag acacctcctg
     5821 cgagagagag ccagcggctc cgcggtacga cgtcgaggaa ccgcagcctg cctgcgacca
     5881 ggtgcaccgc cgccaggacg gcggctgcag ggagcgggaa gagagggatc accggctccc
     5941 cctcgaggcg gcaggggata acggttacct cccgatcttt cgggataccg tacggatgat
     6001 atcgagcagg tttgccagat acgtttcctt cgcctcgagt cttcgccgga gcacggcctc
     6061 ttcggagagg aagagggcct tgagatggcg gatgtcctgt tttggggagt aaaagaggta
     6121 gatcgggatg ccggcgagga tgaccaggac ccctgcgacc ttatcgagca tcgtcgtcga
     6181 gaagaggaga aagaggcaga cgccgatgcc ggcgagggga aggatgtgct ggccgcggag
     6241 atgtgcggcg ttgtcgtctc gcagcacgat gagggcaaag caggtgagca ggaacgagaa
     6301 cgcgagggtg aggacggcaa acgatatcag attggagagg ttcccgatat tggagaggat
     6361 gaacgcgatg accccctggg cgatgaggac cacgtggggc gtgtcgtagc gtgggtggag
     6421 ccgtgcgaag gcgcggggga agagcccgtc gatagccatc gcatacgcga ggcgggcgct
     6481 gccgagcatc cccgactcgt ttgaccccga gaccgagaag agggcgccga ccgtcatgat
     6541 gatcgccccc gccgacccga agagggcggt cccggcgacg acgagcggga cggcgctccg
     6601 gtcgaggagg gtccagttga cgagcccgaa gaggacgaag ttcgtgagga ggtagaagac
     6661 ggcgacgatc agcatcccgg tggtgatggc gcgggggatg gccgtctgcg ggttctctac
     6721 ctcgccggcc gggagcgtcc cgagctcgaa cccggcgtat gcccagaaga ccaggaccag
     6781 ggcgtgggag gcgttatcga gcccgaaagg gagcaggggg gtgtagttcc cgatgaagac
     6841 ttccggccgg atgaggaaga tgccgagacc ggccacgatg aggaggagca ggggtgcgag
     6901 tttgatgagc gtgaggagat cgttcaacct ccctgcggcc cggacgccga cgatgttgac
     6961 gagggtcagg gatgcgacga agagcgcccg gacggctatc tctgcggctg ttgtgagggg
     7021 gacgagcgcc tggaggtagt tcgtgaacgc tatcgcaaag accgggaggg cgaggagttc
     7081 ggctatccac atgctccagc cggtgagaaa cccatagaag tcgtcgaacg cctccgagac
     7141 gtaggcgaac ggccctccga cgcgaggaac gtagtaactg cagtaggcaa agaccatcgc
     7201 gatcgtcgcg gcaaagagcc cggccaccgc ccagaggacg atcgagaacg ggccgacgag
     7261 gcccgccgtg agggcggacg cgatgtagat gtcggcgccg acgatcgccc cgacgatgat
     7321 gttcgtcagg tcgaactgcg agagcccccg ctgtaatccc atgccgcggg aagaggaggg
     7381 ggcagcggcg ataaagattc cgcaccggta tgcgccggcc ccgtgcgcaa ccgccgggca
     7441 aaccctcatc acatccgggg tcgtagaatg gtcgggtgca taagggtgag attccgcatt
     7501 gtcgtcaggg aggatccgga ggatggcggg tatgtggtga gttgccctgc aattcccgga
     7561 tgccactcgc agggcgagac gcttgaagag gctcttgaga acataagcga tgccatatcc
     7621 gggtgcgttg aggtattgaa cgagcgagcg gagtgagcat ccgggcggct cgccgcgaga
     7681 gggtggtcac tctgcgaaag attctaaaaa acagcgcacc ggtgcggtgc tttcgagtgc
     7741 gccgaccggg actcgaaccc gggtttaggc gttggcaacg cctagtgata accactacac
     7801 tatcggcgca caccagtgct ctgcactgtg tgccttacaa agtgggatct cctccataat
     7861 aagtctatcg aatcgcagtc cggggcgggc ggcatcgccc ctgcggtatc cgcacgatga
     7921 ggcgctctca gggcggtatt cgccacccct ccgcgccggc acctaccgcc gcctctgcca
     7981 ccgaagcgcg atccggatgt tccggtttgc aagtatccat agccccggta tcaggaccag
     8041 gacgaatatg accgttgcgt aggcgacgat atcggagagg aaaagcagga ggttgaaggt
     8101 gccgtgcagc atcatcgcga gagcaagccc ccggaatatg atacccggcc gtcgctcggc
     8161 agccgtgaac ttggcccggc cgagggcgta gccccagacg ctggaaaaga gcgcgtgagc
     8221 ggggaccgag aagatcgccc ggatggcgag cgtctcgaag gcaagcgtcg gcgacgtcac
     8281 gtatgccgtg aggacgtaga ggacgttctc gagggatgcg agcccgaggg cggccgcggc
     8341 ggcgtagacg atgccgtcca tcggctcatc gaactcggcg tcccggtaga cgaaccggta
     8401 tacgaccgtg aacttgccgt actcctcgac gatgggcgct atgatgacgc ccatgatcag
     8461 cggggatgcg atgaggaggc cgacgaaccc ctcgacgaag gccacgggga aggtgaccag
     8521 gacaccgagg aggtatacct tcacgatcag ggctgccggc tcgggctcgt acttatccct
     8581 gtggtagaag taccataacc agaatacccc gggccccagc gccaggacaa ggagcaccag
     8641 cggttcgatc gcaaccatta cccggccgta gtcatgttct catcagggaa gagcctttct
     8701 catggccgaa aaaaagggtt actccgggag gagccggtac cccggtccaa gcccttccag
     8761 caccagcagg gcataatacc ggaagaacgt gacgaacggc accgcgatca ggagcgcggc
     8821 cgggatggcg atgatgaggt aggggataag gagaacgacg aacagagcga tgttcccccc
     8881 ctggaatgcg gcgaggagca ctaccccgat gagcacgaac ggtatcgcga tgatcaggag
     8941 cgctatgatg accaggaccg cctgcacgat tgcggcgatg aggcccacca caaacctcac
     9001 aacgacgtag gcggcggtct gccagaaccc ggccgatatc acgccgatga gccgctgcca
     9061 gccctcgatg acgccgcagt cctcgcggat catgatcggg acgacaaagt cggtggtcag
     9121 gaggaatatg agaccgaata cgagagctgc gaccaggatg atcggtatga gcgcgatgat
     9181 caggagcgct gcggcggcgc cggtgccgat actcccgaac ccaaagagta tcaagaagag
     9241 cgccataatg gcgaggacca tggccacggt gatgccgagc tcgaaaagga agagtcttgt
     9301 tcctttgccg agccgttcgc ggaagaaccg cctgatatgg atatccccgg tcgagagcat
     9361 atcgacgaag acgaactgca tcgtagcacc gatcagcatc cagacaagag cgatcgcgag
     9421 cacgacggca atgatcccga gcacaaccat cggtaaaatc tcggggaagg agccggcatt
     9481 accggctaaa aaatcaccct cctcgaattg atactggaag acgtttggta tactgatgcc
     9541 gcctcctaca aagagagcga tcacggcgag gcggagccag atgccccact ttatcggcca
     9601 gagcaggctt ttcatgcgat gaaaagcgcc ttccagcctg ttgaatgcat agatctcttc
     9661 agccatatgt ctcaagaaga aaaggatctc ctcgccttta aaacctcctc cgggcaagcc
     9721 ggcgccggtc tatcggtccg gggacatatg ccggggagcc tgaggggagg ccggtttttc
     9781 aacaccttaa tctattccgt tcaccacact atagaagaat gatccct
//
LOCUS       DAOSEE010000082         9797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_2605_length_9797_cov_212.505235, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000082 DAOSEE010000000
VERSION     DAOSEE010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 9797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9797
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2605_length_9797_cov_212.505235"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PLI76_07280"
     CDS             <1..240
                     /locus_tag="PLI76_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61870.1"
                     /translation="PAAPTPPAPAALLPRPPWGGGSAYDIRWAAVHRIMVTTATGGCH
                     GARAPRRRVAGIAAGALRKGLIGIAGDLMPSPPEP"
     gene            complement(262..1191)
                     /gene="argF"
                     /locus_tag="PLI76_07285"
     CDS             complement(262..1191)
                     /gene="argF"
                     /locus_tag="PLI76_07285"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOI61871.1"
                     /translation="MKKDFLSITDIDGYELESIVADAMYLKRLKSTGTPHEFLRGKSL
                     GMIFEKASTRTRVSFEVGMTDFGGHALFLNPADLQLGRGEAIKDTARTLARYTDAVMI
                     RAYSHATVEEFARYSTVPVVNGLSDRLHPCQVLSDIMTLNERFGDLQDLKLAWVGDGN
                     NVCNTWLLSAAMTGMEIVVASPPRYRPRDAVVDQARAAGGRITIVADPAEAVRDADVL
                     YTDIWVSMGDEKERTERMQALRDYTVDSRLLARAPPDALVMHCLPAHRGEEITDEVID
                     GPQSIVWDQAENRLHAQKALLLRLIAGGMPATE"
     gene            complement(1188..2480)
                     /gene="purD"
                     /locus_tag="PLI76_07290"
     CDS             complement(1188..2480)
                     /gene="purD"
                     /locus_tag="PLI76_07290"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOI61872.1"
                     /translation="MVMKLLVVGGGGREHAITRALSCNSDVKLFSVMARENPGIARLA
                     ERVFLTDETDLPGIVRFAMANGIDVAVIGPEAPLEAGVVDHLEAAGIPCVGPTRAAAR
                     IETDKAFCRRLMERHGIAGCPEYRVFQDPEEAGRFIEAYGGDLAVKPIGLTGGKGVRI
                     MGEHVDVAGAVGYVREIGGDVVLEERLIGEEFTLQAFVDGKHLVPMPLVQDHKRAYEG
                     DVGPNTGGMGSYSLSDHMLPFVTRRDYEEALRIMEDTVAAMRDEGTPYRGILYGQFMN
                     TRDGPKVIEFNARFGDPEAMNVLSILESDLAGIVRHIVEGDLSPAHVRFAHKATVCKY
                     IVPEGYPDAPMAGEPLAPGDCGDALLYYANVEERDGVLYTRTSRTLAFVGRGETLEEA
                     EAIAERAASSVTGRVFHRRDIGTQEVLERRCRHMKEVS"
     gene            complement(2506..3018)
                     /gene="pyrE"
                     /locus_tag="PLI76_07295"
     CDS             complement(2506..3018)
                     /gene="pyrE"
                     /locus_tag="PLI76_07295"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOI61873.1"
                     /translation="MVNPIAKLLLESGAVEFGDFVLASGAPSSYYIDIKAATTNPTIL
                     AAIGKAVAEGWDFDTVAGVAVGGIPIAVAVSLASGRPYAIVRKEEKDHGRAGTIIGDV
                     AGRNVLLVEDVTTSGGSVLYGLAALRAAGARVDRVVTVVDREAGAHEALAEKGTSLFA
                     LVRVSELLNG"
     gene            complement(3012..3512)
                     /locus_tag="PLI76_07300"
     CDS             complement(3012..3512)
                     /locus_tag="PLI76_07300"
                     /EC_number="2.7.7.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol
                     synthase"
                     /protein_id="HOI61874.1"
                     /translation="MLPAYVPNSAAALFGGGTPIDSGRTLGDGRRIFGDGKTYRGFFG
                     GVLAGVLVGLVEILAWSSFGLTALPHQTILSVTLFATGALLGDLAKSFLKRRLGKERG
                     ESWFLADQYDLVIGSFLLILLVYPEWLFENITLPIAVWIVVMTPLLHRAVNIIGYYIG
                     VKEVPW"
     gene            3654..5123
                     /locus_tag="PLI76_07305"
     CDS             3654..5123
                     /locus_tag="PLI76_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOI61875.1"
                     /translation="MLMKKTKSLCPTCGRVLDADIVEEEGKVWLVRTCPVHGEYKALY
                     WSDADMYRKFEEYERIGRGVANPQKTTSPAGCPNDCGLCPNHRSATLLANIDLTNRCN
                     LNCDFCFANAQACGYIYEPTFDQIVDMLRMLREEKPVPVPAVQFSGGEPTMRDDLPEL
                     VLKAKELGLYQVQVATNGIRIAQEPGYAARLKEAGLSTVYLHFDGVTRETNSKLASDR
                     RAIENCEKIGLGVVLVPTIINGRNDHEVGAIIRYAAEHIKTVRGVNFQPVAFTGAASE
                     DDVRKERITIPELADRIEEQTDGVIKKEYFYPVPCVVPISELVEAYTGKPQVTFTTHQ
                     HCGAATYVFITDEGMVPINKMVDVDAFFESVEKMGANLAKGGSLNKYVTLVEGVKDLY
                     VSTRKAEQTNTGELLKLIGRTLIMQNFEALREFHWNALFIGTMHFMDNYNYDISRVQR
                     CCIHYATPDGRLIPFCTYNSGPVYREQVWKAFALPPEDE"
     gene            complement(5205..6143)
                     /locus_tag="PLI76_07310"
     CDS             complement(5205..6143)
                     /locus_tag="PLI76_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843848.1"
                     /note="catalyzes the interconversion of alanine and
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HOI61876.1"
                     /translation="MRYYPVHSGYPPYTRVNQAIEAAFAEHGRGGVRMPSKVYVGLVE
                     NGDFRTMPAYMPALGIAGVKIVNVHPNNRARGLPTVMALTEIIDVTTGVPQAIVNATE
                     LTAMRTGAAGAIAAKYLSPRRTVTLGVVGAGRQAEALVEAIAATLTIDEIRVWSRTEK
                     SSEAFAARYADYNAVSVSIERACDCDVLNTTTPSRKPLIMADWIGEGTHINAIGADAP
                     GKQELDPRILTGAEVFVDDPGQAAHSGEINVPIETGLYDAGMIAGTLGEVVIGKRGRS
                     SPGAVTVFDSTGLAIQDLAIAALIIERDDGYDLPFP"
     gene            complement(6292..6945)
                     /locus_tag="PLI76_07315"
     CDS             complement(6292..6945)
                     /locus_tag="PLI76_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HOI61877.1"
                     /translation="MLPDGISLGRLQGPLSALASISPRFTGAVRIKGPGGTGFILAQG
                     GNPYAAAFEGTGGGLPLRGDEAYRYLLDRPSFDYELIAYTADETGAAREVAREAGCLI
                     SGDGKTPASRANSGETARLDRIARQPGVIAVSVFHEGFALQSLGSADFDQVAAVAEDL
                     LRAGVRITADMDMGDLAQVILETPAGKLIIAPYGDLSLCILAKPDANLGLIRLSIRG"
     gene            complement(6950..7297)
                     /locus_tag="PLI76_07320"
     CDS             complement(6950..7297)
                     /locus_tag="PLI76_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HOI61878.1"
                     /translation="MLRQILGEFLRLDGVTAAVVAGRDGFVIESVVAGDIDVEALGAM
                     ASTGMGTSEAMSNELGKGEMHQMLVELENGPILLSPLSEDELIAIVANANVNVGRIRY
                     ELKKNRDRITAAL"
     gene            complement(7309..8676)
                     /locus_tag="PLI76_07325"
     CDS             complement(7309..8676)
                     /locus_tag="PLI76_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HOI61879.1"
                     /translation="MKEITISPVTRIEGHAGVRIYLNDRGEVDSAHFQVVELRGFEKF
                     LVGAAIEEAPRITPRICGICPSAHHLAAAKATDRIFGVTPPATGKNLRELLGLGQFVH
                     SHALHFFMLAAPDFILSPDAPPEVRNVVGLARHSPDIARKAITVRKFGQRLTEAVGGK
                     PIHPANALPGGMSAPLTEGKRDELAAMADEALGIAREGWEIAKNLLDGVDTEIGAVRT
                     GFMGMTNHGLYSTYDGPVAMLDPDGGRIGSFSGQDYTNFVEEYSEDWSYLKFARLKGG
                     GYYRVGPLARLNIVERMGTEHADAALAEYRERFGTVTQAPLAYHLARYIEFLASCERA
                     KDLLADPGITGSGIRTPAGKVVNRRGVGIIEAPRGTLIHDYTVNDAGIIEKCNLIVAT
                     CQNNYAIDRGVEDMARRVVKNGTLTEGAANRIEMIIRAYDPCISCATHAIGRMPLRIE
                     CMRRT"
     gene            complement(8673..9590)
                     /locus_tag="PLI76_07330"
     CDS             complement(8673..9590)
                     /locus_tag="PLI76_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F420-nonreducing hydrogenase"
                     /protein_id="HOI61880.1"
                     /translation="MKIAIEELAGCSGCTIAVLDLHEMLLDVVAKADIVYSPVIMDVK
                     EPPEGIDIAFVTGAVRNEENRERLEKIRKRAKTLVALGTCACYGGISGLSMLSSNQDL
                     FSCVYRGVETAKPDGVIPTDVPPFLYRAFAVGDIVKIDYYVTGCPPKEAFLKKIIPAM
                     VAGDTLELSRKSVCSECDRTMGPVGDWTLRRRHEGVPDREHCLLGQGYLCLGSVTFGR
                     CGATCPKNNIPCHGCNGPSLDILREPCRDIYGMMVRRISDLTDKPAKEVEKELYDVAH
                     TMYAFTIGSLIMEDKETSKIRDLVKERSR"
     gene            complement(9601..>9797)
                     /locus_tag="PLI76_07335"
     CDS             complement(9601..>9797)
                     /locus_tag="PLI76_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrocarbon binding protein (contains V4R
                     domain)"
                     /protein_id="HOI61881.1"
                     /translation="IYLVFHDCIVRQTLRRNGLDQGGPLCQTLFGYVVGAVEEITGRK
                     ARLEIVHTGPNACLKKLVLK"
BASE COUNT         1758 a         3227 c         3000 g         1812 t
ORIGIN      
        1 ccggcagccc ccaccccgcc cgcgcctgcg gcgctcctcc cccgcccccc ctggggtggt
       61 ggcagtgctt acgatatcag gtgggccgcc gtgcacagga tcatggtgac caccgccacg
      121 gggggctgcc acggcgcgcg cgcgccccgc cggcgggtcg cgggcatcgc tgccggcgcg
      181 ttgagaaagg gcctgatcgg tatagccggc gacctcatgc cttcgccgcc ggaaccctga
      241 aaaaagatac tcttcccgcc gtcactccgt cgcaggcatg ccgccggcga tcagccgcag
      301 gagcagtgct ttctgcgcgt ggagccggtt ctcggcctgg tcccagacga tgctctgcgg
      361 cccgtctatc acctcgtcgg tgatctcctc tccccggtgg gccgggaggc agtgcatcac
      421 gagcgcgtcg ggcggcgccc gggcaaggag gcgggagtct accgtgtaat ccctgagggc
      481 ctgcatgcgc tcggtgcgct ccttctcgtc gcccatggag acccatatgt cggtatagag
      541 gacgtccgca tcccggacgg cttccgccgg gtccgcgacg atcgtgatcc tgccgcccgc
      601 cgcccgcgcc tggtcgacga cggcatccct gggccggtaa cgcggggggc ttgcgacaac
      661 gatctccatc ccggtcatgg ccgcggagag gagccacgtg ttgcagacgt tgttgccgtc
      721 tccgacccat gccagcttca ggtcctgcag gtcgccgaac cgctcattga gggtcatgat
      781 gtcggagagc acctggcagg ggtgcagccg gtcggacaac ccgttgacga ccgggaccgt
      841 cgagtagcgg gcaaactcct cgacggtggc atggctgtag gcacggatca tcaccgcatc
      901 ggtgtagcgg gcaagcgtcc gcgccgtatc cttgatcgct tcgccccgcc caagctgcag
      961 atccgcgggg ttcaagaaga gcgcgtggcc cccgaaatcg gtcataccga cctcaaacga
     1021 gacccgggtg cgggtggacg ccttctcgaa tatcatcccg aggctctttc ccctcaggaa
     1081 ctcatgcggg gtccccgtgg acttcagccg ctttaagtac attgcatcgg caacgatgct
     1141 ctccagttca tacccgtcga tatcagtgat cgagagaaaa tccttcttca tgaaacctcc
     1201 ttcatatgcc ggcaccgccg ttcgaggacc tcctgcgtgc cgatatcccg gcggtgaaag
     1261 acgcgcccgg taacagatga tgccgccctc tcggcgatcg cctcggcctc ttcgagcgtc
     1321 tcgcccctcc cgacgaacgc gagcgtccgc gacgtccggg tgtagagcac cccgtctcgc
     1381 tcctcgacgt ttgcgtagta gaggagggcg tcgccgcagt cgccgggggc gagaggctcg
     1441 ccggccatcg gggcatccgg gtagccctcg gggacgatat acttgcagac cgtcgcctta
     1501 tgggcgaacc ggacatgcgc cggcgagaga tcgccctcga cgatgtgccg gacaatcccg
     1561 gcgagatcgg actccaggat cgagagaacg ttcatcgcct ccggatcacc gaatcgggcg
     1621 ttgaactcga tgaccttcgg gccgtcgcgg gtgttcatga actggccgta gaggatcccc
     1681 cggtacggcg tcccctcgtc ccgcatggcc gcgaccgtat cctccatgat ccggagcgct
     1741 tcctcgtaat ccctccgggt gacgaaggga agcatgtggt cggagaggga gtacgaaccc
     1801 ataccgccgg tgttcggccc cacgtcaccc tcgtatgctc gtttgtggtc ctgaacgagc
     1861 ggcatgggca ccaggtgctt gccgtcgacg aacgcctgga gggtgaactc ttcaccgatg
     1921 agccgctcct ccagcacgac atcccccccg atctcccgga cgtacccgac cgctcccgcc
     1981 acgtcgacgt gttcgcccat gatccgcaca cccttccccc cggtgagccc gatgggcttg
     2041 acggcgagat cgccgccgta ggcctcgatg aaccgcccgg cctcctccgg gtcctgaaag
     2101 acccggtact ccgggcatcc cgcgataccg tgccgctcca tcagccggcg gcagaacgcc
     2161 ttatccgtct cgatacgagc cgcggcccgg gtcggcccca cgcagggtat ccccgccgcc
     2221 tcgaggtggt cgacgacgcc tgcctcaagg ggcgcctccg gcccgatgac cgcaacatcg
     2281 atcccgttcg ccatggcgaa ccggacgata cccgggagat cggtctcatc ggtgagaaac
     2341 acccgctccg cgagccgggc aattcccggg ttctctcgtg ccatgaccga gaagagcttc
     2401 acgtcactgt tgcaggaaag cgccctggtg atagcatgct ccctgccacc accccccaca
     2461 acgagtagtt tcataaccat ctatttgccc gctcttaaaa aatgtttatc cgttcagcag
     2521 ctcgctgacc cgcacaagtg cgaagagcga cgtccctttc tctgcgagcg cctcgtgggc
     2581 gcccgcctcc cggtcgacga cggtcacgac ccggtcgaca cgggcacccg cggcacgcag
     2641 ggccgcaagg ccgtagagaa cgcttccccc cgatgtggtc acgtcctcga cgagcagaac
     2701 gttcctgccg gccacgtcgc cgatgatcgt cccggccctg ccgtgatcct tctcctcctt
     2761 cctgacgatc gcgtagggcc ggccgctcgc aagcgagacg gcgacggcga tcgggattcc
     2821 gccgaccgcc accccggcca ccgtatcaaa atcccatccc tcagcgacag cctttcctat
     2881 cgccgcaagg atcgtggggt tcgtcgttgc ggccttgatg tcgatgtagt aggagctcgg
     2941 ggctcccgat gcgaggacaa agtccccgaa ctcgacggcc ccgctctcca gcaacagttt
     3001 tgcgattggg ttcaccatgg tacctcttta actccgatgt aataaccgat aatgttcacc
     3061 gcccggtgaa ggaggggtgt catgacgacg atccagacgg caatgggtaa agtaatattc
     3121 tcaaagagcc actccggata aaccaggagg atgagcagga atgaaccgat aaccagatcg
     3181 tactggtcgg ccaggaacca ggactcgccc cgctccttgc ccagcctccg tttcaggaag
     3241 ctcttggcga gatcgccaag aagcgcgcct gttgcaaaca acgtaacgga gaggatggtc
     3301 tgatggggaa gtgcggtcag gccgaacgag gaccatgcca ggatctcgac gaggccgaca
     3361 agcacgccgg caagtacacc cccgaagaat ccccggtatg tcttcccgtc gccaaatatc
     3421 cgcctcccat cgccaagcgt cctgccggag tcgataggtg ttccgccgcc gaagagcgct
     3481 gccgctgagt tcggcacgta ggccgggagc atgatccaca gcgccgcgag cgcagggaag
     3541 atccaggagc cgatgtctgc gtcgatcaat gtcaatattc agtacaatca gtaattataa
     3601 agattaaagt gtcgaaatat gagataggaa tgctagatgg gcacggtgag agaatgctga
     3661 tgaaaaagac aaaaagcctc tgtccgacgt gcggccgcgt gctcgatgcc gatatcgtcg
     3721 aggaggaggg gaaggtctgg ctggtccgca cctgtcctgt tcacggcgag tacaaggccc
     3781 tctactggtc ggatgcggac atgtaccgga agttcgagga gtacgagcgc attggaaggg
     3841 gggtggcaaa cccacagaag accacttctc cagcagggtg cccaaacgac tgcggcctct
     3901 gcccgaacca ccggtcggcg acattgcttg caaacatcga cctgacgaac cggtgtaacc
     3961 tcaactgtga cttctgtttt gcaaacgccc aggcatgcgg ctacatctac gaaccgacct
     4021 tcgaccagat cgtcgatatg ctgcgtatgc tgcgcgagga aaaaccggtc ccggtccctg
     4081 ccgtccagtt ctcgggcggc gaaccgacga tgcgcgacga tctccccgaa cttgtattaa
     4141 aggcaaagga gctcggcctc taccaggtgc aggtcgcaac gaacggcatc cggatagcgc
     4201 aggaacccgg ctacgctgca cgactgaagg aggcagggct ctccaccgtc tacctgcact
     4261 tcgacggcgt aaccagagag accaattcaa aactcgcaag cgaccggcgg gccatagaga
     4321 actgcgagaa gatcggtctt ggcgtggtgc tggttcccac gatcatcaat ggccgaaacg
     4381 accacgaggt gggcgccatc atccggtatg ccgcggagca tatcaagacc gtgagaggcg
     4441 tcaacttcca gccggtggcg ttcaccgggg ccgcaagcga ggatgatgtc aggaaagagc
     4501 gtatcaccat ccctgaactt gcggatcgga tcgaggagca gacggacggc gtgatcaaga
     4561 aggaatactt ctatccggtg ccctgtgtcg tcccgatatc cgaactcgtc gaggcatata
     4621 cgggcaaacc ccaggtcacg ttcaccacgc accagcactg cggcgcggcg acctacgtct
     4681 tcatcaccga cgaggggatg gtcccgatca acaagatggt ggacgtcgac gccttctttg
     4741 agtctgtcga gaagatgggg gcgaatctcg ccaaaggcgg gtcgctcaac aagtacgtga
     4801 ccctggtcga gggcgtcaag gatctctacg tctccacgcg gaaggcggag cagacgaata
     4861 ccggggagct cctgaaactg atcggcagaa ccctcatcat gcagaacttc gaggccctgc
     4921 gggagttcca ctggaacgcc ctcttcatcg ggacgatgca cttcatggac aactacaact
     4981 acgacatatc ccgggtgcag cggtgctgca tccactacgc gacccccgac ggccgcctga
     5041 taccgttctg cacctacaac agcggccccg tctaccgcga acaggtctgg aaagccttcg
     5101 ccctgccgcc cgaggacgag taacggctca ccataccgca aaagagaggt ccgccggccc
     5161 tctcccggcc ccttttctcg ataagcacga tttatctgtg aagatcaggg gaacggcagg
     5221 tcatacccat catccctctc gatgatgagg gcggcgatgg caaggtcctg tatggcaagt
     5281 ccggtggagt caaagaccgt gacggcgccg ggagacgacc gtcccctctt cccgatgacg
     5341 acctctccga gggttcccgc tatcataccc gcgtcgtaga ggccggtctc tatcgggacg
     5401 ttgatctcgc ctgagtgggc agcctggccg ggatcgtcga caaaaacctc cgccccggtc
     5461 agtatccggg gatccagttc ctgtttgccg ggagcatcgg cgccgatcgc gttgatatgc
     5521 gtcccctccc cgatccagtc cgccataatg aggggttttc ttgacggcgt cgtcgtgttc
     5581 aggacgtcgc agtcgcatgc gcgctcgatc gagacgctta ccgcgttgta atccgcgtaa
     5641 cgtgctgcaa acgcctcgct actcttctcc gtcctgctcc agacccggat ctcatcgatc
     5701 gtgagcgttg ccgcaatcgc ctcgacgagc gcctccgcct gccggccggc acccacgaca
     5761 ccgagggtga cggtgcgccg gggcgagaga tacttcgccg ctatggcgcc cgccgccccg
     5821 gtgcgcatcg ccgtgagctc ggtggcgttg acgatcgcct gcggtacgcc tgttgtcacg
     5881 tcgatgatct ccgtgagcgc catgacggta ggcaggcctc gtgcacggtt gttcgggtgg
     5941 acgttgacga tcttcacccc ggctatcccg agcgccggca tgtatgcagg catcgtccgg
     6001 aaatccccgt tctcgacgag ccccacgtag accttcgacg gcatccggac tcctcctctg
     6061 ccgtgctctg caaacgccgc ctcgatcgcc tggtttaccc gggtgtacgg cgggtatccg
     6121 gagtgaaccg ggtagtatct catagtgcgg tcacctcacg gcggggcgat tgggccggga
     6181 gcctttcccg ggctcccgcc ggccataccc ctggtacagt ccggagttgc atagctttct
     6241 ctccgatctg ccgggatatc aacctgcggg tacagacccc tctctcctca ctcagccccg
     6301 tatcgagagc cggatcaggc cgaggttggc atcgggtttt gcaagtatgc agagggagag
     6361 gtcaccgtag ggtgcgatga tgagtttccc tgccggggtc tcgaggatca cctgggcaag
     6421 gtcccccata tccatatcgg cggtgatgcg gacgccagcg cggagcaggt cttcggcgac
     6481 cgcggccacc tggtcgaagt ccgcggaacc gagcgactgg agggcgaacc cctcgtggaa
     6541 gaccgagacg gcgatcaccc cgggctgccg tgcgatccgg tcgaggcgag ccgtctcccc
     6601 cgaattagcc cgggatgcgg gtgttttgcc gtccccgctg atcaggcacc ccgcttcccg
     6661 ggctacctcc cgggccgccc ccgtctcgtc ggcggtgtag gcgatgagtt cgtagtcaaa
     6721 cgacggccgg tcgagcaggt accggtaggc ctcgtctccc ctgaggggca ggcctccccc
     6781 ggtaccctcg aacgctgccg cgtaggggtt cccgccctgg gcgaggatga accccgtccc
     6841 cccgggcccc ttgatacgca ccgctcccgt gaagcggggg ctgatggagg caagggccga
     6901 gaggggcccc tgcagccgtc cgagcgagat gccgtcgggg agcatcgtct cacagggcgg
     6961 cggttatcct gtccctgttc ttcttcagct cgtaccggat ccgcccgacg ttgacgttcg
     7021 cgttcgccac gatggcgatc agctcgtcct cggagagcgg ggagaggagg atggggccgt
     7081 tctcgagttc gacgagcatc tggtgcatct cgcccttccc gagttcgttg ctcatcgcct
     7141 ccgacgtacc catgcccgtc gatgccatgg cgcccagggc ctcgacatct atgtcgccgg
     7201 caacaacgct ctcgatcaca aagccgtccc gtcccgccac gaccgcagcg gtaaccccgt
     7261 caagtctcag gaactctccg agaatctgtc gtaacatctc acaccacctc aggttcttct
     7321 catacattcg attctaaggg gcatccgccc gatggcgtgg gtcgcacacg atatgcaggg
     7381 gtcgtatgcc ctgatgatca tctcgatgcg gtttgccgcc ccttcggtca acgtcccgtt
     7441 cttcaccacc cggcgcgcca tatcctcgac accccggtcg atcgcgtagt tgttctggca
     7501 ggtcgcgacg atcaggttgc acttctcgat gatgccggca tcgttcacgg tataatcgtg
     7561 gatcagggtc ccccggggag cctcgatgat cccgacgccc cgccggttca cgaccttccc
     7621 tgccggtgtc cttatgccgg aaccggtgat gccgggatcc gcgagcaggt cttttgcccg
     7681 ctcgcaggag gcgaggaact cgatgtagcg ggcgaggtga taggcaaggg gtgcctgggt
     7741 gacggtgccg aaccgctcgc ggtactccgc gagggcggcg tcggcatgct ccgtccccat
     7801 cctctccacg atgttgagcc gggcaagcgg cccgacccgg tagtagcccc cgcctttgag
     7861 ccgggcgaac ttcaggtagg accagtcttc cgagtactcc tcgacgaagt tcgtgtaatc
     7921 ctggcccgag aacgagccta tccgcccacc gtcggggtca agcatcgcca caggtccgtc
     7981 gtatgtcgag tagagaccgt ggttggtcat acccatgaac ccggtccgga cggcgccgat
     8041 ctccgtgtcg accccgtcga ggaggttctt cgcgatctcc cagccctccc gggcgatgcc
     8101 gagcgcctcg tccgccatgg cggcgagctc atccctcttc ccctccgtga gcggcgccga
     8161 catgccgccg gggagggcgt ttgccgggtg gatgggtttg ccgccgaccg cctccgtcag
     8221 gcgctggcca aacttcctga ccgttatggc cttcctggcg atgtccggcg agtggcgtgc
     8281 aagcccgacg acgttccgca cctccggcgg cgcgtcaggg gagaggatga agtcgggggc
     8341 cgcgagcatg aagaagtgga gggcgtggga atggacgaac tgcccgagcc ccaggagctc
     8401 gcggaggttc tttccggtcg cggggggtgt gacgccgaat atccgatcgg tcgccttcgc
     8461 cgccgcgagg tggtgcgccg aggggcatat gccgcatatc cggggagtga tccggggtgc
     8521 ctcctcgata gccgccccga cgaggaactt ctcgaacccc cggagttcga cgacctggaa
     8581 gtgggccgag tcgacctctc cccggtcgtt gagatagatc ctgacgccgg cgtggccttc
     8641 gatccgggtc accgggctga tcgttatctc cttcatcggc tcctctcctt cacgaggtcg
     8701 cggatcttcg acgtctcctt gtcttccatg atcaggctcc cgatggtgaa ggcgtacatc
     8761 gtatgggcga cgtcgtagag ctccttctcc acctcttttg ccggtttatc ggtgagatcc
     8821 gatatcctcc tgaccatcat gccgtagatg tcgcggcagg gttcgcggag gatatcgagc
     8881 gacgggccgt tgcacccgtg gcagggtatg ttgttcttcg ggcacgttgc accgcacctg
     8941 ccgaaggtga cgctcccgag gcagaggtag ccctgcccga gcagacagtg ttcgcggtcg
     9001 ggaacgcctt cgtgccgccg ccggagcgtc cagtccccga ccggccccat ggtccggtcg
     9061 cactccgagc agacggactt gcgcgagagt tcaagggtat cccccgcaac catcgccggg
     9121 atgatcttct tcaagaacgc ttcttttggg gggcagcccg tgacgtagta gtcgatcttc
     9181 acgatgtcgc ccaccgcgaa cgcccggtag aggaacggcg gcacatccgt cggtataacc
     9241 ccgtcgggtt ttgccgtctc caccccgcgg tagacgcacg agaagaggtc ctggttcgag
     9301 ctcagcatcg agaggcccga tatccccccg tagcaggcgc aggtcccgag agcgacgagc
     9361 gtctttgccc gtttccggat cttttcgagg cgttcccggt tctcttcgtt tctgacggct
     9421 cccgtcacga acgctatgtc gataccctcg gggggttcct tcacgtccat gatcaccggg
     9481 gagtagacga tatccgcctt cgcgacgacg tcaaggagca tctcgtggag gtcgagtacc
     9541 gcgatcgtgc accccgaaca tcctgccagt tcttcaatgg cgatcttcat cgtctccacc
     9601 tcatttcagg accagtttct tcaggcaggc gttcgggccg gtgtggacga tctccagcct
     9661 ggctttccgg ccggtgatct cctcgaccgc gcccacgacg tagccaaaga gggtctggca
     9721 gagcgggccg ccctggtcga ggccgttgcg ccggagcgtc tggcggacga tgcagtcgtg
     9781 gaagacgagg tagatga
//
LOCUS       DAOSEE010000083         9593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_2667_length_9593_cov_256.818096, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000083 DAOSEE010000000
VERSION     DAOSEE010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 9593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9593
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2667_length_9593_cov_256.818096"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1876)
                     /locus_tag="PLI76_07340"
     CDS             complement(<1..1876)
                     /locus_tag="PLI76_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOI61882.1"
                     /translation="MYITQLEIDNFKSFAKKTKIPFFGGFTVISGPNGSGKSNIIDSI
                     LFVLALSGARGLRAEKLTDLINVNTGKNTAEVTATFSDGTAIRRRIKRTPAGYYSYNY
                     LNNRLCKQSEVIEFLAKIGIKPEGYNVVMQGDITRIMEMSDGERRKIVDEIAGVAEFD
                     HKREQAFSELEVVRERIEREEILLSELVARLDALQHEREQAMEYRRWQEELEHLGRCR
                     GAALVRQKEQEIGTLHALTLDQKEALGRTAGEADAVRAQAEEARERRQAVEEEISTKS
                     GPEYLALVGRLEEARGGIKLAEKTIERLAAGRDENLETIQRVYMDGRRAEAKVEGCAG
                     QIRTLSIDRANLAMEASIEREQLAAVEKRIASESKEVEGIKDQLFARMQDLENRKELR
                     AKILREQDILIEKSRMRTSERERLEARTRQIDEELVNKRAQMADYASSMATSETEKRR
                     IERDLSEAESTLFARRSALDRLQKEIRENQQSLMRLEAQQQAQGDAGGKAMDAILDME
                     GVHGTVAQLGRAPPEYATALDVAAAGRLRWVIVDTDGIASDAIRYLKDNRLGRVTFLP
                     LNKLRPPVLSPLEADPGIVGYAVDLLEFDPAFERAFRVVFGATVVVDTLERARRLMGR
                     Y"
     gene            complement(1894..2511)
                     /locus_tag="PLI76_07345"
     CDS             complement(1894..2511)
                     /locus_tag="PLI76_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61883.1"
                     /translation="MAQKDARIRFLERELAEREKEMETMTEPEQPAAPAVNDERLRDL
                     ERKVRELEALVRGLTEEILDLKSVTMKLSREAEERRVKSVTAESKKAGATLQAEPRAA
                     VEPRVAAVPREPARPTEKQRIAADQSDANMELIMQNDGTLKPESRRSSEYIVASNKFG
                     NAPMKGQSRSRGGKSSDRTLFVEQKKREVENVIQAEEDDTVDLDR"
     gene            2700..3971
                     /gene="glyA"
                     /locus_tag="PLI76_07350"
     CDS             2700..3971
                     /gene="glyA"
                     /locus_tag="PLI76_07350"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOI61884.1"
                     /translation="MSYLANFDPEVAGIIENERLRQINGLELIASENVVSKAVLEATG
                     SIMTNKYAEGYPGKRYYGGCEYHDAVENLARDRLCKLFGAEHANVQPHSGSQANQAVY
                     FAYLNYKDRIMSQSLTQGGHLSHGSPVNITGRWYSIFHYGVDSVTETLDYAVIEDMAR
                     IAKPQIIVCGASAYPREIDFKAFQEIADTVGARCMADIAHIAGLCATGYHNSPVGVVD
                     IVTTTTHKTLRGPRGGAIMCGKENAAAIDKSIFPGMQGGPLMHIIAAKAVCFKEALTP
                     AYGDYCGQVVRNARTMADVLTEEGLDLVSGGTDNHLILLDLTGVSTNHEHLTGLAAET
                     ALGEAGITVNKNTIPREQLSPFVTSGLRIGTPAVTSRGMKEEEMKQIGHWIARVVKDV
                     ARDRTSKKAITEVREEVIALASKYPLYPEVA"
     gene            3968..4819
                     /gene="folD"
                     /locus_tag="PLI76_07355"
     CDS             3968..4819
                     /gene="folD"
                     /locus_tag="PLI76_07355"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOI61885.1"
                     /translation="MILDGKAVSEKRLELLKEKIDESGLYPRLATVIVGEDPASQMYV
                     RMKHRACERVGIGSIGIELSIDASTEQVLEAVARLNTDPDIDGILVQLPLPRGVETAR
                     VIDAVAPIKDVDGFHPFNLGRLLAGKPVYAPCTPQGVMTILDEYGIPIAGKRAVVVGR
                     SIDVGRPMAVLLLNADATVTICHSRTENLADETRRADILVSAVGKAKFVRPDMVKAGA
                     TVIDVGINQDEQGRLSGDVDFEAVRERAGAITPVPGGVGPMTIATLMENTFKAARLRT
                     CCDEIVR"
     gene            4795..5610
                     /gene="folP"
                     /locus_tag="PLI76_07360"
     CDS             4795..5610
                     /gene="folP"
                     /locus_tag="PLI76_07360"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOI61886.1"
                     /translation="MLRRDCTINRLRIGGDAPVRLMGVINCSPESFYRGSYTPAGRVY
                     DRALAMIDAGADLIDLGARSTAPGSPPITVAEEAARAEAALSELDGTGITVSVDTRYA
                     EVLDLALRHDIHAVNDISGLADERYARAVADSGLPVFVMASLREPGDAVGLAATLAAL
                     DAVVARCTRLGIEEYVLDPAVGRWVPGRTSEDDWGLCRNFEAFSSFGRPVLAAVSRKT
                     FIGDLLGREPDDRLAGTLALTAMLVEKGAAVVRSHDVRETADLLRVSAKMRGR"
     gene            5607..6413
                     /gene="cofE"
                     /locus_tag="PLI76_07365"
     CDS             5607..6413
                     /gene="cofE"
                     /locus_tag="PLI76_07365"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HOI61887.1"
                     /translation="MTAPSFSVYGLATALIRAGDDVAAHLIEAAARAGCEGFQTGDVV
                     VIAESAVATAEGRAVQLAGIEPSDEARRLAENYRMDPCQVEVVLRESDRVVGGIPGFL
                     LCMKNGTLLPNAGIDASNTPRGSVLLLPADPDASAARIRATIRERSGADVGVIVIDSR
                     THAMRLGCSGVAIGCSGIPSVLDERGKQDLFGRELEVTKRAVADCIASAAELVMGEAN
                     ECVPAAVVRGLMLPVGDYTGVATIDASECLFMGVALHADPSLLVDDKREP"
     gene            complement(6339..6986)
                     /gene="cofC"
                     /locus_tag="PLI76_07370"
     CDS             complement(6339..6986)
                     /gene="cofC"
                     /locus_tag="PLI76_07370"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HOI61888.1"
                     /translation="MYFHALIPFKPVNPKTRLSCILDQEEREAFARAMLEDVIASVLK
                     SGCSATLLCTHPFKHENALIAVRKESLNDAINWALGQFHCPALIIMADIPLVTAGDIQ
                     RLIRTEKDMAIVPGRGGGTNIIFLKKPRCFRADYYGASFLDHMRIAGECGFSVEVIDS
                     FRMSTDIDEKEDLVEILVHGKGRKSREYLENSGFSLVIDEKGRVGVQRDAHKETL"
     gene            complement(7108..7338)
                     /locus_tag="PLI76_07375"
     CDS             complement(7108..7338)
                     /locus_tag="PLI76_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61889.1"
                     /translation="MPFDELRNTVAKYLKTARDVEEFRITLAVLESGAWRLNLSWIEK
                     IEDFKWPRRAGMKVNANTGALLEFRYSYNYSH"
     gene            7518..8513
                     /gene="cofG"
                     /locus_tag="PLI76_07380"
     CDS             7518..8513
                     /gene="cofG"
                     /locus_tag="PLI76_07380"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HOI61890.1"
                     /translation="MHRRVITFSRNAFLPLTTVCRNRCGYCCFRTPVREGCVMPPDDA
                     LRTMEASARLGCTEALFTFGERPGAVAGFDAVLGRIGYADILDYVYDLSLAAIERGLL
                     PHTNAGILSYDELDRLRGVNASMGLMLETTADIPAHRNSPGKDPAVRIDMIENAGRLS
                     IPFTTGILVGIGETMEDREESLSVIRDLHVRFGHIQEVIVQNFCPKPGTLMEGAPAPG
                     TDELCATIALAREVLPADVAVQIPPNLADASHLVGCGVDDLGGVSPLTIDYVNPERPW
                     PQLEELRRVAGDAELRERLCIYPQYIEKGWYPPLLEPLIRRLAARIAAPGGGEGA"
     gene            8556..9281
                     /locus_tag="PLI76_07385"
     CDS             8556..9281
                     /locus_tag="PLI76_07385"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOI61891.1"
                     /translation="MKQVDLLYSGKAKSVYRTDDPGVYIMKFRDDITAFDGGKKDTLG
                     GKGRYNAEVSSFFFRYLEENGIKTHYLSSVDPTAIAVRSLTMIPLEVIVRNVAAGSIV
                     RNYPFREGEVLDPAVIVIDYKSDAHHDPMLNDDLIYALGLATPEELDQIKAMALAVNI
                     VLSEYLAARGITLVDFKLEFGKTDGEIVVGDEISMDSMRLWDKETGASLDKDVYRFNK
                     GDVMETYAGVAKRILSPPGEESA"
BASE COUNT         1712 a         3157 c         2974 g         1750 t
ORIGIN      
        1 tgtagcggcc catcagccgc cgcgcccgct cgagggtatc caccaccacc gtcgcgccga
       61 agacgacgcg gaaggcgcgc tcgaacgccg ggtcgaactc aagaaggtcg actgcgtacc
      121 cgacgatgcc cgggtccgcc tcgagcggcg agaggacggg cgggcgaagt ttgttgagcg
      181 gcagaaaggt gacccgcccg aggcggttgt cctttaagta gcggatcgcg tcggacgcga
      241 tcccgtcggt atcgacgatc acccagcgga gccttccggc cgccgccacg tcgagagcgg
      301 tcgcgtactc cggcggcgcc cggccaagct gcgccacggt gccgtggacc ccctccatgt
      361 cgaggatggc gtccatcgcc ttcccgccgg catcgccctg cgcctgctgc tgcgcctcaa
      421 ggcgcatcag gctctgctgg ttctcccgga tctccttttg cagccggtca agagccgagc
      481 gccgcgcaaa gagcgtgctc tcggcctcgg agaggtcgcg ctcgatccgg cgcttctcgg
      541 tctcggaggt ggccatggag gaggcgtagt ccgccatctg cgcccgtttg ttcaccagtt
      601 cctcgtcgat ctggcgggtg cgcgcctcca ggcgctcgcg ctccgacgtc cgcatccggc
      661 tcttctcgat caggatatcc tgctcgcgga ggatctttgc ccggagctcc tttctgttct
      721 cgaggtcctg catccgcgcg aagagctggt ccttgatccc ctcgacctct ttgctctcgc
      781 tcgcgatacg tttctcgacc gccgcaagct gctcgcgctc tatcgatgcc tccatcgcga
      841 ggtttgcccg gtcgatcgag agggtgcgga tctggccggc gcatccctcg accttcgcct
      901 cggcgcgcct gccgtccatg tagacccgct ggatggtctc gaggttctcg tcccggccgg
      961 ccgcgaggcg ctcgatggtc ttctcggcga gtttgatccc gccccgtgcc tcctcgagcc
     1021 gcccgacgag cgcgaggtat tcgggaccgc tctttgtgct gatctcctct tcgaccgcct
     1081 gccggcgctc gcgcgcctct tcagcctgtg cccggacggc gtcggcctcg ccggcggtgc
     1141 gcccgagcgc ctccttctgg tcgagcgtga gcgcatgcag ggtaccgatc tcctgctcct
     1201 tctgccggac gagcgcagcc ccccggcagc ggccgaggtg ctcgagttcc tcctgccacc
     1261 gccggtactc catcgcctgc tcgcgctcgt gctggagcgc atccagtcgt gccacgagtt
     1321 cggagaggag gatctcctcg cgctctatcc gctcccggac cacctcgagc tccgagaacg
     1381 cctgctcgcg cttatggtcg aactcggcga cacccgctat ctcgtcgacg atctttcgcc
     1441 gctctccatc gctcatctcc atgatgcggg tgatatcgcc ctgcatcacg acgttgtagc
     1501 cttccggttt gatcccgatc ttcgcgagga attcgatgac ctcgctctgc ttgcagaggc
     1561 ggttgtttaa gtagttgtaa ctatagtacc ccgccggggt ccgcttgatc ctgcggcgaa
     1621 tcgccgtgcc gtccgagaac gtcgccgtca cctcggcggt gttcttcccg gtgttgacgt
     1681 tgatgagatc ggtcagcttc tcggcccgca gcccccgcgc acccgagaga gccaggacaa
     1741 agaggatgct gtcgatgatg ttgctcttcc ccgagccgtt cgggcctgag atgaccgtaa
     1801 accctccaaa aaaagggatt ttcgtctttt tcgcgaagga cttgaagttg tcgatctcaa
     1861 gctgcgtgat atacaaggat cgggctcccc tgtctacctg tcgagatcga ccgtgtcgtc
     1921 ctcttcagcc tggatgacgt tctcgacctc ccgcttcttc tgctccacga agagggtccg
     1981 gtcggatgac ttgccgcccc ggctccggct ctggcctttc atgggagcgt tcccgaactt
     2041 gttgctggca acgatgtact ccgaggatct ccgcgattcc ggtttcagcg tcccatcgtt
     2101 ctgcatgatg agttccatgt tcgcgtcgct ctggtctgcc gctatccgct gcttctcagt
     2161 cggtcgcgcg ggctcccggg gcacggccgc aacacggggt tccacggcgg cgcgaggttc
     2221 tgcctggagc gtcgccccgg ccttcttgct ctcggccgta accgacttaa ccctgcgctc
     2281 ctccgcttcc cgggagagtt tcatcgtcac ggacttgaga tccaggatct cttccgtcag
     2341 gcccctgacc agggcctcga gctcccgcac cttgcgctcc agatcgcgca ggcgctcatc
     2401 gttcacggcc ggtgccgccg gctgttcggg ttctgtcatc gtctccatct ccttctcgcg
     2461 ttccgcaagt tccctctcaa ggaaccgtat cctcgcatct ttctgtgcca ttgtctccct
     2521 cgaattaatc cttgataata attacgtaat aatattacta tggatttccg aaaagaagaa
     2581 tgattacatt ttatccggga tctgccggac accctgccgg aagtcaccgg ataggcagtc
     2641 gacgcggaca aattctgcaa aagaaagaat attacatccc cagagcaacc ttttcctgca
     2701 tgtcttatct ggcaaacttc gatccggaag tcgcaggcat catcgagaac gagcgcctgc
     2761 gccaaatcaa cgggttggag ctgattgcct cagagaacgt cgtcagcaag gcggttctcg
     2821 aggcgaccgg ctccatcatg accaataagt acgccgaggg ctaccccggc aaacgttact
     2881 acggcggttg cgagtaccac gacgccgtgg agaacctggc gcgcgaccgg ctctgcaaac
     2941 tcttcggcgc ggagcacgca aacgtccagc cgcactcggg aagccaggca aaccaggccg
     3001 tctacttcgc ctacctcaac tacaaggaca ggatcatgag ccagagcctc acccagggcg
     3061 gccacctctc ccacggctcg ccggtcaaca tcaccgggcg ctggtactcc atcttccact
     3121 acggcgtcga ctccgtgacg gagacgctcg actatgcggt catcgaggat atggcgcgga
     3181 ttgcaaagcc ccagatcatc gtctgcggcg caagtgccta cccgcgcgag atcgacttca
     3241 aagcgttcca ggagatcgct gacaccgtcg gtgcgcggtg catggccgat atcgcccaca
     3301 tcgccggact ctgcgcaacc ggataccaca actccccggt cggcgtcgtc gatatcgtga
     3361 cgacgacgac gcacaagacc ctgcggggcc cccggggcgg cgccatcatg tgcggcaaag
     3421 agaacgccgc cgccatcgac aagtccatct tccccggcat gcagggcggt ccgctgatgc
     3481 acatcatagc cgcaaaggcg gtctgtttca aggaagcgct gacccccgca tacggggact
     3541 actgcgggca ggtcgtcagg aacgcacgca cgatggccga tgtcctcacc gaggaagggc
     3601 tcgatctcgt ctccggcggc accgacaacc acctgatcct cctcgacctg accggggtct
     3661 ccacgaacca cgagcacctc accggccttg cggccgagac cgctcttggc gaggccggca
     3721 tcaccgtgaa caagaacacc atcccccgcg agcagctcag ccccttcgtg acgagcggtc
     3781 tacggatcgg cacgccggcc gtcacctccc gcggcatgaa agaagaggag atgaaacaga
     3841 tcggccactg gatcgcccgg gtcgtgaagg acgtcgcccg ggaccggacg tcaaagaagg
     3901 caatcaccga ggtgagggaa gaggttattg ccctcgcaag caagtatcct ctttatcctg
     3961 aagtagcatg atactcgatg gaaaagcggt ctctgagaag aggctcgagc tcctcaagga
     4021 gaagatagac gagtccgggc tctacccgcg ccttgcgacc gtcatcgtcg gagaggatcc
     4081 ggcatcgcag atgtatgtcc ggatgaagca ccgggcgtgc gaacgcgtcg ggatcgggtc
     4141 catcgggatc gagctttcga tagatgcctc cacggagcag gtgctcgagg cggtcgcgcg
     4201 cctcaacacc gacccggata tcgacggcat cctggtccag ctgccgctcc cccggggcgt
     4261 ggagaccgcc cgcgtcatcg atgcggtcgc gcccataaag gatgtggacg ggttccaccc
     4321 cttcaacctg ggccgcctcc ttgccgggaa accggtctac gccccctgca ccccgcaggg
     4381 ggtcatgacg atcctcgatg agtacggtat cccgatcgcc gggaagcggg cggtcgtcgt
     4441 cggccggagc atcgatgtgg ggaggcccat ggccgtcctg ctcttgaatg ccgatgcgac
     4501 cgtcacgata tgccactcca ggaccgaaaa cctcgccgat gagacgcgga gagccgacat
     4561 cctggtcagc gcggtcggaa aggcgaagtt tgtcaggccg gatatggtaa aagccggcgc
     4621 gacggtcatc gatgtcggga tcaaccagga cgagcagggc agactctccg gcgacgtcga
     4681 tttcgaggcg gtgcgcgagc gggcgggggc gataaccccg gtccccggcg gcgtcggccc
     4741 catgacgatc gcgacgctga tggagaacac cttcaaggct gccaggttga ggacatgctg
     4801 cgacgagatt gtacgataaa ccgcctccgg atcggcggcg acgccccggt tcgcctgatg
     4861 ggcgtcatca actgcagccc cgagtcgttc taccggggtt cctacacccc ggccggccgg
     4921 gtgtacgacc gggctctcgc catgatcgat gcgggcgccg acctgatcga cctcggggcg
     4981 cggagcacgg ccccgggttc tcccccgatc accgtcgccg aggaggcggc gcgggcggag
     5041 gcggccctct cggaactcga cgggaccggt atcaccgtat cggtggatac ccgctatgcg
     5101 gaggtgctcg atctcgctct ccgccacgat atccacgcgg tcaacgacat atccgggctt
     5161 gccgacgagc gctacgcccg ggcggtcgcc gactccggtc tcccggtctt tgtcatggca
     5221 agcctccggg agcccggcga tgcggtcggt cttgccgcaa ccctcgccgc gctcgatgcg
     5281 gtcgtggcgc ggtgcacgcg cctcgggatc gaggagtacg tcctcgaccc cgctgtcgga
     5341 agatgggtcc cggggaggac gagcgaggac gactgggggc tctgccggaa cttcgaggcg
     5401 ttctcatcct tcggccgccc ggtgctcgcc gcggtctcgc gaaagacgtt catcggggac
     5461 ctgctcggga gggagcccga cgatcggctt gccggaacgc tcgcgctcac ggcgatgctg
     5521 gtggagaagg gggcggccgt cgtgcggagc cacgacgtcc gcgagaccgc cgacctcctg
     5581 cgggtgtccg cgaagatgag gggacgatga cggcgccatc gttctcggtc tacgggcttg
     5641 cgaccgcgct gatacgtgcc ggcgacgacg tcgcggccca cctcatcgag gcggccgctc
     5701 gtgcggggtg cgaagggttt cagaccggcg atgtcgtggt catcgccgag tcggccgtgg
     5761 ccaccgccga ggggcgggcc gtgcagctcg ccggcatcga gccgtccgac gaggcgcgca
     5821 ggctcgccga aaattaccgt atggacccct gccaggtcga ggtggtcctc cgcgagagcg
     5881 accgggtcgt cgggggcata ccggggttcc tgctctgcat gaagaacggg acgctcctcc
     5941 cgaacgccgg gatagacgcc tcgaacactc ctcgcggttc cgtcctcctc ctgccggccg
     6001 accccgacgc atcggcggcg cggatacgcg cgacgatccg ggagcggtcg ggggcggacg
     6061 tcggcgtcat cgtcatcgac tcgaggacgc acgcgatgcg ccttggatgc agcggggtcg
     6121 ccatcgggtg ttcgggtata ccgtcggtgc tcgacgagcg cgggaagcag gacctcttcg
     6181 gccgcgagct tgaggtcacc aaacgggcgg tggcggactg tatcgcgtcc gccgcagaac
     6241 tcgtgatggg ggaggcaaac gagtgcgtcc ccgcagccgt ggtgcggggg cttatgctcc
     6301 cggtcggcga ctataccggg gtcgccacca tcgacgcgtc agagtgtctc tttatgggcg
     6361 tcgcgctgca cgccgacccg tcccttctcg tcgatgacaa gagagaaccc tgagttctcc
     6421 aggtactccc ggctcttcct cccttttcca tggacaagga tctcgaccag gtcctccttc
     6481 tcgtcgatgt cggtcgacat ccggaacgag tcgatcacct cgacggagaa accgcactcc
     6541 cctgcgatcc gcatgtggtc gaggaagctt gcgccgtagt agtcggcgcg gaaacaccgg
     6601 ggtttcttta agaatatgat gttcgtcccg ccgccccggc ccggcacgat ggccatgtcc
     6661 ttctcggtgc ggatcagccg ctggatatcc ccggccgtca ccagggggat gtcggccatg
     6721 atgatgagcg ccggacagtg aaactgaccg agcgcccagt tgatcgcgtc gttcaacgac
     6781 tctttcctga cggcgatgag cgcgttctcg tgcttgaacg ggtgggtgca gaggagggtg
     6841 gcgctgcacc ccgacttcag cacggaggcg atcacatctt caagcatcgc ccgggcgaac
     6901 gcctcccgct cttcctggtc gaggatgcag gagaggcgcg tcttggggtt gaccggctta
     6961 aaggggatga gcgcgtggaa gtacatcggg aaaatcgttg tcggggcagc ctcaaaaagg
     7021 catcgctgga tgcgtaccgg agagcccccc acccgcctgc tttatgtcct ctgcgtgccg
     7081 gtgcggcgaa gcgccgggtg ccgcgcttca gtgtgaatag ttgtaagaat accggaactc
     7141 taacagagcg ccggtattcg cattaacttt cattccagcc cgtctcggcc atttaaaatc
     7201 ttcaatcttt tcaatccagg agagattcag cctccaggcg ccgctttcta aaacagccaa
     7261 cgtgatcctg aactcttcaa catcccgggc tgttttcaga tatttcgcca ctgtgttgcg
     7321 cagttcgtca aaaggcaaat tctctccggg tatactgacc gccccccaat gatgataacc
     7381 ggattccggg acaaaaaagc gttttcctca tgccggatat cccggcgtgc agaggggtgc
     7441 gccgcctcgc gcagatcgcc cgcgagagac ggcatcgcca tccctacctt caagtggagg
     7501 gatgccgaca tatacccatg caccgccgcg tgattacctt ctcacgaaac gcgttcctcc
     7561 ccttaacgac ggtctgcagg aaccgttgcg gctattgctg tttccggacg ccggtacggg
     7621 agggatgcgt catgcctccc gacgatgcgc tccgcaccat ggaggcgagc gcacgcctcg
     7681 gctgcacgga ggcgctcttc accttcgggg agcgcccggg agcggtggcg ggattcgacg
     7741 ccgtgctcgg caggataggg tatgccgaca tactcgacta cgtctacgac ctctcccttg
     7801 cggccataga aagggggctc ctcccgcaca ccaacgccgg catcctctcc tacgacgaac
     7861 tcgaccggct ccgcggcgtg aacgcgagca tggggctgat gctcgagacg accgcggata
     7921 tccccgccca ccgtaactcc cccggaaaag acccggcggt ccggatcgac atgatcgaga
     7981 acgccggcag gctctcgata ccgttcacga ccgggatcct ggtcgggatc ggcgagacga
     8041 tggaggaccg cgaggagtcg ctctcggtca tccgggacct tcacgtgcgg ttcggccaca
     8101 tccaggaggt gatcgtgcag aacttctgcc cgaaacccgg gacgctgatg gagggtgcgc
     8161 cggcgcccgg caccgacgag ctctgcgcga cgatcgccct tgcccgcgaa gtcctcccgg
     8221 cagacgtcgc ggtgcagata cccccgaacc tcgcggatgc atcgcacctg gtcgggtgcg
     8281 gcgtcgacga cctcggcggg gtctccccgc tcaccatcga ctacgtcaac cccgagcgcc
     8341 cctggccgca gcttgaagaa ctgaggagag tggcgggaga cgccgagctg cgggaacggc
     8401 tctgcatcta cccgcagtat atcgagaaag gctggtatcc accgcttctc gaacccctga
     8461 tccggcgtct cgcggcacgg atcgcggcac ccggcggagg tgagggagca tagtctcatc
     8521 atacatcccg atcaacacct gggaggagat ctggcatgaa acaggttgac ctcctctact
     8581 cagggaaggc gaagtccgtc tatcgcaccg acgacccggg cgtctacatc atgaagtttc
     8641 gcgacgacat cacggccttc gacggcggga agaaagatac tctgggcggc aaagggagat
     8701 ataacgcaga agtctcctcg ttcttcttca ggtacctgga ggagaacggg ataaagacac
     8761 actacctctc aagcgtcgac cccaccgcca tagcggtgcg gagcctcacg atgatcccgc
     8821 tcgaggtgat cgtgaggaac gttgcggccg gttccatcgt gcgcaactat ccgttcaggg
     8881 agggagaggt gctcgatccg gcagtgatcg tcatcgatta caagagcgac gcgcaccacg
     8941 acccgatgct caacgacgac ctgatctatg ccctcgggct tgccacgccc gaggaactcg
     9001 accagatcaa ggcgatggcg ctcgccgtaa acatagtgct ctcggagtac ctcgccgcgc
     9061 gcggcatcac cctggtcgac ttcaagctcg agttcggcaa gaccgacggc gagatcgtcg
     9121 tcggcgacga gatcagcatg gactcgatgc ggctctggga taaagagacc ggggcatccc
     9181 ttgacaagga tgtctaccgc ttcaacaagg gggacgtcat ggagacctac gccggcgtcg
     9241 cgaaacggat actctcgccg cccggagaag agtccgcgtg agccataccg cgaccgtcgc
     9301 cgttgcaccc cggggagaga tgcccggcac cggggtgcga tccctctctc cggggaggaa
     9361 aaccggcatg ccgccctgaa ccgtgagaga ggggtcgcgc tccgtccctc tgatgggcac
     9421 ggcaacgcga cggcggggag agcctgaacg ggccggcctt ctctccggag caggaaccac
     9481 cctcgcaacg atgccacgcc ccgggcatgc ccgcagaggg cagggtcaaa atctttaact
     9541 tgatgacaac ccatatcgaa gagtacggac ggcaggaggc ctgattaaga gta
//
LOCUS       DAOSEE010000084         9269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_2775_length_9269_cov_223.324506, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000084 DAOSEE010000000
VERSION     DAOSEE010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 9269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9269
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2775_length_9269_cov_223.324506"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..976)
                     /locus_tag="PLI76_07390"
     CDS             complement(5..976)
                     /locus_tag="PLI76_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOI61892.1"
                     /translation="MVKVAVVEGDGIGREVVPAARDILAAVRPDIEFFDVDLGYGLWE
                     RTGCACGEETVADLGSADAILFGAVTTPPDPGYRSVMLQIRHALDLYANVRPIRGDTV
                     DVVIVRENTEGLYSGIEWTEPDRSCTVRVVSRRGSERIARYACTLAKSRRHHLTVGNK
                     ANVLKSDCLFVEVCTAVAAHLGVPCTARYIDALSLDLLMHPDRYDVIVTTNMFGDILS
                     DAAAYLVGGLGMLPSANIGERAALFEPVHGSAPDIAGRNVANPIAAIRSGAMLLRHVG
                     DPASAAAVEEAVVRVLRAGIRTRDIGGTAGTREFGAAVLREVGRGKA"
     gene            complement(954..1778)
                     /locus_tag="PLI76_07395"
     CDS             complement(954..1778)
                     /locus_tag="PLI76_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61893.1"
                     /translation="MIFPPHCKYVGAVTGTPCGERVYFLSRYLVRETEPDGMEVLEVE
                     ADPAGTGLMRSILSTRVLAAGDEVCRYPDPVNVQDRTFLVRAALRSGHRCTVFSGHGC
                     QTTFVLDPDLSGFLRLHVYDITPPRPHLSATLGDLERTGLFGDLEVIFEHHVRDIREI
                     EADVYPCRAAGFPRTLDADPVRPGDRVAGCLTAKDLVRECYGEEIGVESICPLGAVTA
                     EPFVARCCRSERGGLGRWNGKFGAVVHWGASSWEIADAVRQVAAAWRERNGEGSGR"
     gene            complement(1775..2245)
                     /locus_tag="PLI76_07400"
     CDS             complement(1775..2245)
                     /locus_tag="PLI76_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HOI61894.1"
                     /translation="MQGTGPAVCLGNDVDTDLIIAGRYLRTKDRSVWAEHVLEDLDPA
                     LAPRIRGAIIVAGRNMGCGSSREQAVVALREAGVVGIVAESFARIFFRNAVNVGLPVI
                     EASVACTDGAHVAFDLDSGWVEVDGVRRPARPLSPRMVTILRAGGLVPYWRSCR"
     gene            complement(2245..3456)
                     /locus_tag="PLI76_07405"
     CDS             complement(2245..3456)
                     /locus_tag="PLI76_07405"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HOI61895.1"
                     /translation="MSTLSERILGAPAGAYVDREVDIAFAHDGTGVLTREALREMGVE
                     QLPHPERLRLVFDHIVPANTGTTATLQAELRGYARASGIDLSDAGGGICHQVMSEGVV
                     RPGMVVVGADSHTCTLGALGAFATGVGATDMAAIWASGATWFRVPETIAVNLTGRLGG
                     PAEPKDVALACVARLGMEGATYRALEFVGDGASGISMDGRLTLCNMAVETGAKTGIFY
                     ADAVTVRHLAGHGVAASPQAVEEPRYEREIAIDLSGIVPLVAVPHRVDTVREAEDVAG
                     TPIDQVFVGTCTNGRYEDLARLARIVRGRKVAVRTLVVPASRAVLARAIATGVLADIV
                     DAGCMVAPPGCGPCLGAHEGVMGEGEVCLSTANRNFKNRMGVGAEIYLSSVATAAVSA
                     LAGEIAVPEVA"
     gene            complement(3453..4583)
                     /locus_tag="PLI76_07410"
     CDS             complement(3453..4583)
                     /locus_tag="PLI76_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase family protein"
                     /protein_id="HOI61896.1"
                     /translation="MKPWNIEICDVTLRDGEQTPGVSFTHDEKMTIARALDEIGVEVI
                     EAGFPVVSSAEKECVAAIARSGLSARVCCLARALRPDVEAALDCDVDMVSIFIATSDL
                     HIRHKYRKPRAEVLDDALDMVEFAADHGVQVRFAAEDASRTDPAFLIEMYSRGAECGA
                     DLVSFADTVGCLAPLEIHAVISRILGDVHHPLCIHCHNDLGFAAANTITAAAAGAFQL
                     HTTVNGIGERAGNAALEQVLVALRMKGGVDRYDLTRLQEISRLVARCSGVVPERTRPI
                     VGENAFAHESGIHIAAILEDPATYESILPELVGGERRFVLGKHTGRKALEHIAQAYGF
                     DLSDGQVRWVLEQIKQRSEGKCSVTPEVLCGFLRQAKAEVLS"
     gene            complement(4555..5811)
                     /locus_tag="PLI76_07415"
     CDS             complement(4555..5811)
                     /locus_tag="PLI76_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOI61897.1"
                     /translation="MKGEEALARAILRSADRCYTVPGYPVSDLAGRVGAAITANEKVA
                     VEYALGDSLSGRRAAVIVKHVGLNACADPLVHATAQGLRAGVVIVVGDDLRAAASDVV
                     QDSRYYGEVARVPVLEPDGETIGPAVEAAFEASETFSRIAIVRVTPGLLDADVPETPA
                     PRSGREGSLADPGLTMAGRAQAADRRTAAMVAWSRASPLNRYEGDVFHAVTVYPPPAD
                     PDVLARTLEAGRPFLREHRLLEPPATTGEPERFSARGYSRTFCRDCPFHPALAILSER
                     RMRTICDAGCAILAMNPPYRVGLATYGLGSSVAVAATGPGIALAGDYALLHSGLAALI
                     DVYERGLPLLCIVLANNRMGMTGGHPVPDILRYIAWADPVVCAADDTGALRRALTLPE
                     EGPRTVVIKGTCPEGAHHETLEYRDL"
     gene            complement(5808..6776)
                     /locus_tag="PLI76_07420"
     CDS             complement(5808..6776)
                     /locus_tag="PLI76_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOI61898.1"
                     /translation="MEWIRLKARLLEAGSVRLSGEPADQYISRSAAGPSAGAPGSLFF
                     SLGDRRVRVGIDETAPIEVIHRGEGVADLLIGGDMVRGRLEAAALHCPRQAYVTVSGT
                     CIFHCRYCTVPHLPGRRKGIAEVVGMVESVADRIDAIAITSGVASSIEEEEAYVLDVV
                     AALAPFGLPIGVSIYPGPETPARLRALGVVEVKFNLEAATETLFRDMCPGLSWEAVHE
                     ALFRSVALFGRGRVFSNIIVGLGETDGDLERVMTDLAAAGVIPVLRPLTPAASLAGRP
                     RPPADRLLRLSAAHERILREAGLDPRRALTMCPACTGCDLVPGRDT"
     gene            complement(6758..7651)
                     /locus_tag="PLI76_07425"
     CDS             complement(6758..7651)
                     /locus_tag="PLI76_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1743 domain-containing protein"
                     /protein_id="HOI61899.1"
                     /translation="MERLSDPYTIRYPQIVGVADESGGHVELIEFFDCTGGAMWVKRH
                     YAQSPLVCSVRTVGATNRYILSAGSADLALEGSVFPAGIAAVAVQGDEIAVTYRGLGG
                     GGVGASICRASATGVVRHKSDPAGGGRLAGSTIWLPRRERVVIGIDDTDTPEEGATWT
                     LAHNIARAVEDDRSRYLSHTIVQLYPVPYRTKNCVAIACEFATSDPGGLARHYRDLLT
                     EYTLSEETGMAVWRGFDTAPLEEFGGKVKRGEVSADDLAAITDGRLSVLMDGRGAIGA
                     VAAIPFFTRYEEALALWNGSG"
     gene            complement(7635..8591)
                     /locus_tag="PLI76_07430"
     CDS             complement(7635..8591)
                     /locus_tag="PLI76_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Ile2) 2-agmatinylcytidine synthetase"
                     /protein_id="HOI61900.1"
                     /translation="MITLTPDDVRARFGPLFSMKYLAMVDQNAGLAEIREHCRARGTI
                     EWDAANRIRAGGAIRSCHVEGTTMTMLARLGLSPAKFGAAGREIGGQALEGVEVDGDE
                     VVTTWSGIAGAGVGVAACLTQAPGVIRAEYPSEDDLRIGGARVCRVRIVSPLYEKVTI
                     GIDDTDTREEGATWVLALKCAEACTIPGVEYLDMRLVQLNPAVPKKTTNCVGSALNFA
                     VRPGRVDALLEYVRDFIESEAVSKDTGIAVYRGIAFAEESSYARRVKTELLTLEEAEA
                     EAARMGVRFIDSNRRKGRIGALGAVLWGNKGVEAAGLYGETF"
     gene            complement(8884..>9269)
                     /locus_tag="PLI76_07435"
     CDS             complement(8884..>9269)
                     /locus_tag="PLI76_07435"
                     /EC_number="2.3.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867620.1"
                     /note="catalyzes the transfer of a formyl group from
                     formylmethanofuran to tetrahydromethanopterin
                     tetrahydromethanopterin; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formylmethanofuran--tetrahydromethanopterin
                     N-formyltransferase"
                     /protein_id="HOI61901.1"
                     /translation="AALMAAQAAVDAISGVCGVITPFPGGIVASGSKVGSKKYKFMGA
                     STNEVYCPTLRDKVEGSKVPEGVKAVYEIVIDGVDQDSVRTAMAEGIRAASKVPGVKF
                     ISAGNFGGSLGPFRFELKDVLADYL"
BASE COUNT         1505 a         3211 c         3170 g         1383 t
ORIGIN      
        1 gcgtttaggc cttcccccgc ccgacctcgc ggagcaccgc cgccccgaac tcccgggtgc
       61 cggcggtccc gccgatgtcg cgggtccgga tgcccgcacg gagcacccgc acgaccgcct
      121 cctcgacggc tgcggcggat gcagggtcgc cgacgtggcg aagcagcatc gcaccgctcc
      181 gtatcgccgc gatggggttt gcgacgttcc tccccgctat gtcgggagcg ctcccgtgca
      241 cgggctcaaa gagggcggca cgttctccta tgttcgcgct cgggagcatg ccgagccccc
      301 ccacgaggta cgccgcggcg tcggagagga tgtccccgaa catattggtc gtcacgatca
      361 cgtcgtagcg gtcggggtgc atgaggaggt caagcgagag cgcatcgatg tagcgggccg
      421 tgcaggggac tcccagatgg gcggccaccg ccgtgcagac ctcgacgaag aggcagtccg
      481 actttaagac gtttgccttg ttccccacgg tgaggtggtg ccggcgggac ttcgcgaggg
      541 tgcaggcgta gcgggcgatc cgctcgctcc cccggcggga gacgacccgg acggtgcagg
      601 agcggtcggg ctcggtccac tcgatgccgg agtagagccc ctcggtgttc tcccggacga
      661 tgacgacgtc gacagtgtcg ccacggatgg ggcggacgtt tgcgtagagg tcgagggcgt
      721 ggcggatctg gagcatgacg ctccggtaac cggggtccgg cggcgtcgtg acggccccaa
      781 agaggatggc atccgccgat ccgaggtcgg caaccgtctc ctccccgcag gcgcacccgg
      841 tccgctccca gagcccgtaa cccagatcga cgtcgaagaa ctcgatgtcg gggcggacgg
      901 ccgcaagaat atcgcgagcg gccgggacga cctcgcgccc gatcccgtcg ccctcaacga
      961 ccgctacctt caccattccg ctccctccac gcggccgcga cctgcctgac cgcatccgca
     1021 atctcccacg acgatgctcc ccagtggacc accgcgccga acttgccgtt ccagcgcccg
     1081 agaccgcccc gctcgctccg gcagcaccgg gcgacgaaag gttctgccgt caccgctccg
     1141 agcgggcaga tgctctccac gccgatctcc tccccgtagc actcccgcac caggtctttc
     1201 gccgtcaggc atcccgcgac ccggtcgccc gggcggaccg ggtccgcatc gagggtgcgg
     1261 gggaaaccgg ccgcgcggca gggatagacg tcggcctcga tctccctgat atccctgacg
     1321 tggtgctcga atatgacctc gaggtcgccg aagagaccgg tcctctcaag gtcgccgagc
     1381 gtggcggaga ggtgggggcg gggcggggtg atgtcgtaga cgtgaaggcg aaggaacccc
     1441 gagaggtcgg ggtcgagcac gaacgtggtc tgacagccgt ggccggagaa gaccgtgcac
     1501 cggtgcccgg accggagggc cgccctcacc aggaacgtgc ggtcctggac gttcaccggg
     1561 tccgggtaac ggcagacctc gtcgccggcg gcgagcaccc gggtggagag gatgctcctc
     1621 atcaaaccgg ttccggccgg atcggcctcg acctcgagca cctccatgcc gtccggttcg
     1681 gtctcgcgga ccaggtagcg cgagaggaag tagacccgct ccccgcacgg tgtgccggtc
     1741 acggccccga cgtatttgca atgcggggga aatatcaccg gcacgacctc cagtagggga
     1801 ccagtcctcc ggcccggaga atggtgacca tcctcggcga gagcgggcgg gcggggcggc
     1861 gcaccccgtc cacctccacc cacccgctat cgaggtcgaa agcgacgtgg gcgccatcgg
     1921 tgcaggcaac ggaggcctcg atcaccggga gacccacgtt gacggcgttc cggaagaata
     1981 tgcgggcaaa cgactccgca acgatcccga cgacaccggc ctcccggagg gcgacaaccg
     2041 cctgctcccg cgacgacccg caccccatgt tcctcccggc gacgatgatc gcgccgcgga
     2101 tacggggggc aagcgccggg tccaggtcct cgaggacgtg ctccgcccag accgagcggt
     2161 ccttcgtccg gaggtagcgg cccgcgatga tgaggtcggt gtcgacgtcg ttgccaaggc
     2221 agacggccgg cccggttccc tgcatcatgc cacctccggc acggcgatct cgcccgcgag
     2281 cgcactcacc gcggcggtgg cgaccgatga gaggtagatc tcggcgccca cgcccatccg
     2341 gttcttgaag ttccggttgg cggtcgagag gcagacctcg ccctccccca tcacgccctc
     2401 gtgcgcccca agacacggcc cgcacccggg cggggccacc atgcatcccg catccacgat
     2461 atcggcaagg acgccggtgg cgatcgcccg ggcaagcacc gcgcgggagg cggggacgac
     2521 cagggtgcgg accgccacct tcctcccccg gacgatccgg gctagccggg caaggtcttc
     2581 gtagcggccg ttcgtgcagg tcccgacgaa gacctggtcg atcggcgtgc ccgcgacatc
     2641 ttcggcctcc cggacggtgt ccacccggtg cgggaccgcg acaagaggca cgatgccgga
     2701 gaggtcgatg gcgatctccc gctcgtagcg cggttcctcc acagcctggg gcgacgccgc
     2761 aacgccgtgc ccggcaagat ggcggacggt gaccgcatcg gcgtagaaga tgccggtctt
     2821 tgctcccgtc tcgaccgcca tgttgcagag ggtcaaccgc ccgtccatgg atatccccga
     2881 cgcgccgtca cccacgaact ccagcgcccg gtaggtcgcc ccctccatgc cgagtctggc
     2941 gacacaggca agagcgacgt cttttggctc tgcggggcca ccgagccttc ctgtaaggtt
     3001 gaccgcgatc gtctccggca cccgaaacca cgtagccccc gacgcccaga tcgccgccat
     3061 atcggtcgct cccacgccgg tggcaaacgc cccgagagcg ccgagggtgc aggtgtgaga
     3121 gtcggcgccc acgacgacca tgccgggccg gacgacccct tcgctcatca cctggtggca
     3181 gatccctccg ccggcatccg aaagatctat ccctgaggcc cgggcatacc cgcggagctc
     3241 cgcctggagc gtcgccgtcg tgccggtgtt ggccggcacg atgtggtcga agacgaggcg
     3301 caggcgctcg gggtgaggga gctgctccac gcccatctcc cggagcgcct cccgggtgag
     3361 gacgccggtc ccgtcgtggg caaaggcgat atcgacctcg cggtccacgt atgcgccggc
     3421 aggggcaccg aggatccgct ccgagagcgt gctcacgaga ggacctccgc ctttgcctgc
     3481 cggaggaacc cgcagagcac ctcgggggtg acgctgcatt tcccctcgct cctctgtttg
     3541 atctgctcga gcacccaccg cacctgcccg tcggagaggt cgaacccata cgcctgcgcg
     3601 atatgctcaa gcgctttcct ccccgtatgc ttcccgagga cgaaccggcg ctcgccgccc
     3661 accagttcgg ggaggatgga ctcgtaggtc gccggatcct caaggatggc ggctatgtgg
     3721 atgccgctct cgtgggcaaa ggcgttctcg ccgacgatcg gccgggtgcg ctccgggacc
     3781 acgcccgaac accgggcgac cagccgcgag atctcctgca ggcgggtgag atcgtagcgg
     3841 tcgacgccgc ctttcatccg cagggcgacc agcacctgtt caagcgcggc gttccccgca
     3901 cgctccccga tgccgttgac ggtggtgtgg agctgaaacg ccccggcagc cgccgccgtg
     3961 atggtgtttg cggccgcaaa cccgagatcg ttgtggcagt ggatacagag cgggtgatgg
     4021 acgtcgccaa ggatccggga tatgaccgcg tggatctcaa gcggtgcgag gcatcctacc
     4081 gtatcggcga aactgacgag atcagcgccg cattctgcgc cccgggagta catctcgatg
     4141 aggaacgcag gatcggtccg ggaggcatcc tcggccgcga accgcacctg cacgccgtga
     4201 tcggcggcga actccaccat gtcgagagca tcgtcgagca cctcggcgcg gggctttcgg
     4261 tacttgtgcc ggatgtggag gtcggatgtc gctatgaaga tgctgaccat atccacatcg
     4321 cagtcgaggg ccgcctcgac gtcgggcctg agcgcccgtg ctaggcagca gacccgggcg
     4381 gagaggccgc ttcgcgcgat cgcggcgacg cactctttct ccgccgacga caccacgggg
     4441 aacccggcct cgatcacctc cacgccgatc tcatcgagcg ctcgtgcgat cgtcatcttc
     4501 tcatcatggg taaacgagac gccgggagtc tgctccccgt ctcgcagcgt tacatcacag
     4561 atctcgatat tccagggttt catggtgtgc accttcaggg caggttccct tgatcaccac
     4621 cgtccggggg ccctcctccg ggagggtgag ggcacggcga agagcgcccg tgtcgtctgc
     4681 agcgcagacg accgggtccg cccaggcgat gtagcgaagg atatcaggga cggggtgacc
     4741 gccggtcatc cccatccggt tgttcgcaag tacgatgcag aggagcggga gccctcgttc
     4801 gtagacgtcg atgagggcgg cgagccctga gtgcaggaga gcgtagtcgc cggcgagcgc
     4861 gatgcccggg ccggtcgccg ccacggcaac cgacgacccg aggccgtagg tggcaagccc
     4921 tacccggtag ggcgggttca tcgcgaggat agcgcatccg gcgtcgcaaa tggtccgcat
     4981 cctgcgttcg gagaggatgg caagggcagg gtggaacggg cagtcccggc agaacgtccg
     5041 ggagtagccc cgcgcggaga accgttccgg ctctccggta gtggcgggag gctcgagcag
     5101 ccggtgctcc cggaggaagg ggcggccggc ctcgagggtc cgggcgagca cgtccggatc
     5161 cgccggcggc gggtagacgg tgacggcgtg gaacacatcc ccctcgtaac ggttgagggg
     5221 cgaggcccgc gaccaggcaa ccatggccgc cgtccggcgg tctgccgcct gcgcccgccc
     5281 ggccatcgtg agccccgggt ccgcgaggct cccctcccgg ccgctccggg gtgcgggggt
     5341 ctccgggaca tccgcatcca ggaggccggg cgtgaccctg acgatggcga tgcgggagaa
     5401 ggtctccgag gcttcgaacg ccgcttcgac cgcggggccg atcgtctcgc cgtcgggttc
     5461 cagcaccggc acccgggcga cctcgccgta gtagcgggag tcctgcacga cgtctgatgc
     5521 ggccgcgcgc agatcgtcgc cgacgacgat aacgacgccg gcccggagac cctgggccgt
     5581 tgcatgcacg agcgggtccg cgcaggcgtt gaggccgacg tgcttgacga tcacggcggc
     5641 cctccgcccg gagagcgagt cgccgagggc gtactcgacc gccaccttct cgtttgcggt
     5701 gatcgcggcg ccgactcgcc cggcaagatc cgataccggg tagccgggga cggtgtagca
     5761 ccggtcggcg gaccggagga tggcgcgggc gagcgcctcc tcgcccttca tgtgtccctc
     5821 ccggggacga ggtcgcaccc cgtgcatgcc gggcacatcg tcagcgcccg gcgggggtcg
     5881 agccccgcct ccctgaggat ccgctcgtgg gcggcagaga gccggagcag ccggtcggcg
     5941 gggggccggg gccggccggc gagggacgcc gccggggtga gcgggcgaag caccggtatg
     6001 accccggccg cggcgagatc ggtcatcacc cgctccaggt ccccgtcggt ctccccgagg
     6061 ccgacgatga tgtttgaaaa gaccctgccc ctcccgaaga gcgccacgga ccggaaaagc
     6121 gcctcgtgga ccgcctccca cgaaaggccg gggcacatat cgcgaaagag cgtctccgtc
     6181 gccgcttcga ggttgaactt cacctcgacg acgccgaggg cacggagccg ggccggggtc
     6241 tccgggcccg ggtagatcga gacgccgatg gggagaccga agggcgcgag cgccgcgacg
     6301 acgtcgagga cgtaggcctc ctcctcctcg atcgacgagg cgacgccgct cgtgatcgcg
     6361 atggcgtcga tccggtcggc cacgctctcg accatcccca cgacttcggc gatccccttc
     6421 cgccggccgg ggaggtgcgg caccgtgcag tagcggcagt gaaagatgca cgtcccgctg
     6481 acggtgacgt aggcctggcg cggacagtgg agcgcggcgg cctcgagcct gccgcggacc
     6541 atgtcgccgc cgatgaggag gtcggcaacg ccctctcccc ggtggatgac ctcgatgggg
     6601 gccgtctcat cgataccgac ccggacccgc ctatcgccga gagaaaagaa gagcgaaccg
     6661 ggcgcccccg cagaggggcc ggcggcggag cgggagatgt actgatccgc gggttcgccg
     6721 gagaggcgga cagagcccgc ctcgagcagg cgggctttca gccggatcca ttccatagcg
     6781 ccagcgcctc ctcgtatcgc gtgaagaacg gtattgccgc caccgccccg atcgcccccc
     6841 ggccgtccat gagaacggag agccttccat cggttatggc ggcgagatcg tctgccgaga
     6901 cctcgccccg cttcaccttt ccgccgaact cttcgagcgg tgcggtgtca aacccgcgcc
     6961 agaccgccat accggtctct tccgagaggg tgtactccgt aaggaggtcg cggtagtgcc
     7021 gggcaagccc accggggtcg gaggtcgcga actcgcaggc gatggcgacg cagttcttgg
     7081 tccggtaggg gacgggatag agctggacga tggtgtggga gaggtagcgg gaacggtcgt
     7141 cctcgacggc ccgcgcgatg ttgtgcgcga gcgtccaggt ggcgccttcc tccggcgtat
     7201 cggtgtcgtc gatcccgatg accacgcgct cgcggcgggg gagccagatc gtcgagcccg
     7261 caagacgccc gccgccggcg ggatcgcttt tgtggcgaac gacgcccgta gccgatgcgc
     7321 ggcagatgga tgcccccacg ccgccgccgc cgaggccccg gtaggtgacg gcgatctcgt
     7381 cgccctggac ggcgacagcg gcgatccccg ccgggaagac cgaaccttcg agagcgaggt
     7441 cggcgctgcc ggccgagagg atgtaacggt tggtcgcgcc cacggtgcgg acggagcaga
     7501 cgagcgggct ctgcgcgtag tggcgcttca cccacatcgc gccgccggtg cagtcgaaga
     7561 actcgatgag ttcgacgtgc ccgccgctct cgtcggccac ccccacgatc tgcgggtagc
     7621 gtatcgtgta gggatcagaa agtctctcca tagagccctg ccgcttcgac gcccttgtta
     7681 ccccagagga cggcgccgag tgcccctata cggcccttcc gccggttcga gtcgatgaac
     7741 cggacaccca tccgggcggc ctccgcctcc gcctcttcaa gcgtcaggag ttcggtcttg
     7801 acccttcgtg cgtaggaaga ctcctctgca aacgcaatcc cacggtatac ggcgatgccg
     7861 gtgtctttgc tgaccgcttc ggactcgatg aagtcgcgga cgtactcgag gagggcgtcc
     7921 actctccccg gccggacggc gaagttcagg gcggaaccga cgcagttggt ggtcttcttc
     7981 gggacggcgg ggttcaactg cacgaggcgc atgtccaggt actccacccc ggggatcgtg
     8041 caggcctcgg cgcacttgag cgcgagcacc cacgtggcgc cctcctcccg ggtgtcggta
     8101 tcgtcgatcc cgatcgtcac cttctcgtag agcggcgata cgatccggac ccggcatacc
     8161 cgggcgccgc cgatacggag gtcgtcctcg gagggatact cggcccggat cacccccggc
     8221 gcctgggtca ggcaggcggc gacgccgaca ccggccccgg cgatacccga ccaggtcgtc
     8281 accacctcgt ccccgtcgac ctcgactccc tcgagcgcct gtccgccgat ctcccttccg
     8341 gccgccccga acttcgccgg agatagcccc aggcgcgcaa gcatcgtcat cgtcgtgccc
     8401 tcgacgtgac aggaccggat ggccccgccc gcccggatgc ggttcgccgc atcccactcg
     8461 atcgttcccc gcgcccggca atgctcgcgg atctccgcaa ggccggcgtt ctggtcgacc
     8521 atggcaaggt acttcatcga gaagagaggc ccgaaccggg ccctgacgtc atcgggtgtg
     8581 agcgtgatca tgatgtcacc gaaataatcg ttaacaagaa tttcgttgat aacgatattt
     8641 aaggggatcg atcgcgaggg ccggggcgtg cgagggggcg cacgagggtc atgcgcaccg
     8701 tatgcccggc atcgggggcg agccggcggg catggggagc aggccgacat caatcatatg
     8761 ggcccggcat acccgggcac gaccgtgtcc gcaccatggt gcgccgcacc cgcccgggcc
     8821 ggggccggag gcttatccgt gcgatccagg gccccgaacc gtggataggc aacaaaaaaa
     8881 ggtttaaagg taatccgcaa ggacatcctt gagctcgaac ctgaacggtc cgaggctgcc
     8941 gccgaagttc ccggcgctga tgaacttcac gcccggcacc ttcgatgccg cccggatgcc
     9001 ctcggccatc gcggtccgga ccgaatcctg gtcgacgccg tcgatgacga tctcgtagac
     9061 cgccttgacg ccttcgggca ccttgctccc ctcgacctta tcgcggaggg tcgggcagta
     9121 gacctcgttc gtgcttgcgc ccatgaactt gtacttcttg ctcccgacct ttgagccgct
     9181 cgcgacgatg ccgccgggga agggcgtgat gacgccgcag acgccgctga tggcgtcgac
     9241 cgccgcctgc gcggccatga gcgcggcca
//
LOCUS       DAOSEE010000085         9223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_2791_length_9223_cov_220.376309, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000085 DAOSEE010000000
VERSION     DAOSEE010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 9223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9223
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2791_length_9223_cov_220.376309"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PLI76_07440"
     CDS             complement(<1..662)
                     /locus_tag="PLI76_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61902.1"
                     /translation="MSSLPYPWVIAAVAVLVAAGATFALLNQPATDDRAASALLIRAQ
                     SDITVALETLDGRLSHAASRLGATGLNDTAARAVLSNLAATDPAIVDCTASNPEGTLV
                     AAEPAAYHGAEGTDLRDQPNVLYVLSSKRPIMSEVITVAEGFPAAVISTPVFTNEGRF
                     AGFTSVVFRPEVLIGNAVGPAVNGTPFQVMVVQTDGRVLYDTDPVQVGRMTFEDPLYA
                     DYP"
     gene            complement(746..1462)
                     /locus_tag="PLI76_07445"
     CDS             complement(746..1462)
                     /locus_tag="PLI76_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HOI61903.1"
                     /translation="MTVDHTTSAPGPARWKAFLQNAHLTGLIKFVLPLALIPAIFAAL
                     YLTEPYQQFLVLSGLLVAYFVPPAGKETIIPIAILMGYPWWLIMLVIFLLDVAVSLFV
                     VWNFDLALKIPFIGRLLESGMAVARNYTESQPWLRRLSTVGLILFVFFPLQGTGAMNG
                     SILGRLLGLDESRVFGCVCIGSFASSLIIALGADVLIDIYQEDPVLGIGLLVLIVIII
                     LAGVVGWRMRDRKLRGRVPR"
     gene            1577..1843
                     /locus_tag="PLI76_07450"
     CDS             1577..1843
                     /locus_tag="PLI76_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61904.1"
                     /translation="MAPAPSARSPPRTPAVESGTRESALCTIANVGTALETLREVRRH
                     VRGDHKRRLDDVAVILRVIALEAQATYAITDEEARAFIRECRSR"
     assembly_gap    1894..1993
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2078..2626
                     /locus_tag="PLI76_07455"
     CDS             2078..2626
                     /locus_tag="PLI76_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOI61905.1"
                     /translation="MNEPLRQVVHLLFGLGIAGFILAFDRDFTLSVLVLTLFMGFILS
                     DAVSRGYTIPVISTIIINLERRDAAPGRGALFFALGALFCLVFFEREIVFAGLVVLSL
                     LDSVTTLAGTRFGKTRIYNHKSLEGTLAGVAVTTVALCLLVPPAVALVTAAAAAVAEL
                     VSPVDDNLVIPVVACLALTLLL"
     gene            complement(2874..4373)
                     /gene="pyk"
                     /locus_tag="PLI76_07460"
     CDS             complement(2874..4373)
                     /gene="pyk"
                     /locus_tag="PLI76_07460"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOI61906.1"
                     /translation="MMLEEVLQRISEQAKRKRLTLPDQKTKIVCTIGPTSRSPEVLAG
                     LARAGMNVARLNLSHGTFDEHRENIRNIRAAAEETGLPITILIDLPGPKIRIGDLAHE
                     PLELEKGEVVTLTTAPVSDDPSRIPVEFPQFPQLVSEGSTVFLNDGFLQLRVDNVAEK
                     DVRATVVIGGPLLSRKGMSLVGGGGIVALSAVTDRDLECIDFGLNEGLDTFSISFIER
                     AEDIRKARAYAAGSGKSIRVIAKIERQEALHHLDEILGETDGVMIARGDLGVQTPIEE
                     VPAVQKRIIQKANILGRPVITATQMLMSMTDNIRPTRAEVTDVANAILDGTDAVMLSE
                     ETSIGKYPVETVAMMAKIARSTEEKRTAVGGRCAPLSYFREGKGRERITVNDVVSLNV
                     IEALDALNIRFVLTPTRSGSTPRNISRFKPGAWILAFTRNPATSRFLAFSYGVCPFLI
                     RSEKEYWHGAILDSVRDSGLIRPGERVVLTEGVSPGREGTDSLIILTVD"
     gene            complement(4489..5682)
                     /locus_tag="PLI76_07465"
     CDS             complement(4489..5682)
                     /locus_tag="PLI76_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOI61907.1"
                     /translation="MTTPLLETDNVAYTYPDGTAALAGVSIRIAAGSKTALVGPNGAG
                     KSTLLLMLNGMLRPASGEVRFSGRPIAYDRRSLRELRRQVGFVFQNPDVQIIAPTVEA
                     DVAFGPVNLGLSSDAVRRAVKGALGYVGLDGYERRPPHHLSGGEKKRVAIAGILAMDP
                     AVLVFDEPTNTLDPASSEEVMELLDELASSGRTVLISTHDVELAYRWADSVVLMERGS
                     ILARGSPEEVFSDHDLLAAARLKPPALLDLYNELGLLGIVAPGTPPKCVLEFTDRIER
                     AMHGRAPARCEPGAISVCDADRIDAAHIRALVERGTVAHVGAMGTRAKEFAGREQIFL
                     DYTYGVIDKCILKALIGENSLIITTGGMVEHARRRIGEYSAGSGRAIRVTTVQEPAND
                     LAARE"
     gene            complement(5679..6455)
                     /gene="cbiQ"
                     /locus_tag="PLI76_07470"
     CDS             complement(5679..6455)
                     /gene="cbiQ"
                     /locus_tag="PLI76_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HOI61908.1"
                     /translation="MHEVLEDFARTNALRQVNPGLKLFVGLASILLSVSSPGPVAPLL
                     VAASMSAAVLLLARIPPRVYARLLLIPVSFALMSIAVVLFITGGGAVLLEVPGLPLAV
                     TAGGANLAVLLLARVFGGMCSLYFIALTTPMTEVFDILRRLGVPTVLLDIAMLTYRFI
                     FILIEEAGQIYRSQVMRLGYGRFRESVNSFGMLAGALFIRTWEGGEALVLAMDARCYD
                     GKLDIPGELRPISLLALAAALLYTAIILGVLLSTGGIALI"
     gene            complement(6488..6778)
                     /locus_tag="PLI76_07475"
     CDS             complement(6488..6778)
                     /locus_tag="PLI76_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HOI61909.1"
                     /translation="MKYGMEIAVAALIVIFAAVFLFQDAAIRASGEEAWGGADSNAAD
                     LIEASGYEPWVEPFWEPPSGEIESLLFALQAAIGAIVIGYIFGYWQGGRKAA"
     gene            complement(6775..7467)
                     /locus_tag="PLI76_07480"
     CDS             complement(6775..7467)
                     /locus_tag="PLI76_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HOI61910.1"
                     /translation="MHIMEGFLPGPWWEIWFLVSLPFIVIGLYQVNRLVQEKREALPL
                     LAVAGAFVFVLSSLKLPSFNGSCSHPTGTGLGAILFGPCIAAVLGLIVLVFQAVFLAH
                     GGLTTLGANVFSMGIAGPAVAYIVYRAGIRAGANTYATVFVAAALADLATYVVTSVQL
                     ALAFPGTTGFFGAFLTFAAIFAATQVPLAIIEGAVIALVFKYIVQLKPEILTRLNLLS
                     ESTIQKLREVPA"
     gene            7599..8930
                     /locus_tag="PLI76_07485"
     CDS             7599..8930
                     /locus_tag="PLI76_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HOI61911.1"
                     /translation="MCSIPRIIVAGTHSGCGKTTLASGLMAALTARGLAVQPFKVGPD
                     FIDPTHHSAICGRTSRNLDPFMMGEAGVRETFVRTSAGADVAVVEGAMGLYDGLEGSD
                     TASTAHVAKILDAPVILVVDAGGASRSVHAMALGYAGFDPGVRVAGVILNQIGSARHR
                     AMIEETKSLPIYGGVPRRKDLAVESRHLGLAMAAEDDAMAQFGAVMEETCDLSRIVDL
                     ARSAPSLPAPAEGGSRSETRVRIGVAQDAAFCFYYADNLDRLARAGADLVFFSTVADR
                     LPDVDALYLGGGYPELHAAALAASRCREDIRRAADDGMPIYAECGGLIYLAEQLATDG
                     GDYPMAGILPAAAVMTDRIQALGYVEARVAGGTPVLIPGSAFRGHEFHYSRLDCAPDA
                     RFALTLLRGKGIDGGRDGLIAQNAVGQYTHAYFTEGFAGMLVQAALAYRRS"
     gene            complement(8945..>9223)
                     /locus_tag="PLI76_07490"
     CDS             complement(8945..>9223)
                     /locus_tag="PLI76_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61912.1"
                     /translation="PPAAGGESVSITDRGFVPDVLTVSQGTTVTWTNNAAENETVTAT
                     GQAADFDSGVLQPGDSYNFTFATPDNYSYMSLPTGFTGLIVVTGNTTS"
BASE COUNT         1646 a         2838 c         3097 g         1542 t
ORIGIN      
        1 ggatagtccg cgtagagcgg gtcctcaaac gtcatccgcc cgacctggac cgggtcggtg
       61 tcgtagagca cccgcccatc cgtctggacg accatcacct ggaagggagt tccgttcacc
      121 gccgggccga cggcgttgcc gatcaggacc tccggccgga agacgacgga ggtaaacccg
      181 gcaaaccggc cctcgttcgt aaagaccggc gtgctgatca ccgccgccgg gaacccctcc
      241 gcgaccgtga tcacctcgct catgatcggg cgtttcgacg agaggacata caggacgttg
      301 ggctgatcgc ggagatctgt cccctccgcc ccatgatacg ccgccggttc ggcggcgacg
      361 agcgtgcctt ccgggttgga ggccgtacag tcgacgatcg caggatcggt agcggcgagg
      421 tttgagagga ccgcgcgggc ggcggtgtcg ttcaggcccg tggccccgag gcgggacgcc
      481 gcatggctca ggcggccatc cagcgtttcg agtgccacgg tgatatcgga ttgtgcccgg
      541 atcaggagcg ctgatgcggc acgatcatcg gttgccggct ggttcagcag cgcaaatgtg
      601 gcgccggcag cgaccaggac cgccacggca gcaatgaccc atgggtatgg cagagaactc
      661 attctatccc tctcgtagag agatggggag cgggaaatgt tagaactgtc gatttatcgc
      721 cggaagagag cccgcgccgg caccctcacc ggggtacccg gcccctgagc ttccggtccc
      781 gcatccgcca tcccaccacc ccggcaagga tgatgataac gatcaggacg agcagcccga
      841 taccgagcac cggatcctcc tggtagatat cgatgaggac atcggcgcca agcgcgatga
      901 tgaggctgga tgcgaacgac ccgatacaga cgcaaccaaa gacccgggat tcatcgagcc
      961 cgagcagcct tcccagtatc gacccgttca tcgccccggt cccctggagc gggaagaaga
     1021 caaagaggat gagcccgacc gtcgagagcc gccggagcca gggctgtgac tcggtgtagt
     1081 ttcgggccac cgccataccg ctctcgagca accggccaat gaacgggatc ttcagcgcga
     1141 gatcgaagtt ccagacgacg aagagggaga cggcaacgtc gaggaggaag atcacgagca
     1201 tgatgagcca ccaggggtaa cccatcagga tcgcgatcgg tatgatcgtc tccttgcctg
     1261 ccgggggaac gaagtaggca acgagcaggc cggagagaac gaggaactgc tggtagggtt
     1321 cggtcaggta gagggctgca aagatggccg gtatgagggc gagcggaagg acgaatttta
     1381 tgaggccggt gaggtgtgcg ttctggagga aggccttcca ccgggccggc ccgggggcgc
     1441 tggttgtatg gtccacggtc atggatgaaa ctcccggggt gggagatact cagttctggt
     1501 ttgaggtttc tggataaata cgttcgcgga gaacgttcca gcaggttcaa atctccggag
     1561 cgtgagagta agcaggatgg ctcctgcccc ttccgcccgg tcgccaccgc gcacccccgc
     1621 cgtcgagagc ggcacccggg agagcgcgtt atgcacgata gcaaacgtcg gaaccgcgct
     1681 tgagaccctc cgcgaggtga gaagacacgt ccgcggcgac cacaagcgcc gcctcgacga
     1741 cgtggcggtg atcctccggg tgatcgcgct cgaggcccag gcgacctacg cgatcaccga
     1801 tgaggaggcc cgggcgttca tacgggagtg ccgttcgcgg tgagaccgcc gggcggcgcc
     1861 ggcccctcgc aaagggcctt attgccggca atcnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnaaggcct gtccgggtgt ctaaaacacc cttctgcagc tcccgcctgc
     2041 agagggttta accggctccg caccaacctc tgatacaatg aacgaaccgc tccgccaggt
     2101 cgtccacctc ctcttcggcc tcgggattgc gggatttatc ctcgccttcg accgcgactt
     2161 caccctctcc gtcctcgtgc tcaccctctt catggggttc atcctctccg acgccgtctc
     2221 ccggggctac accatcccgg tcatatcgac gatcatcatc aacctcgagc ggcgggacgc
     2281 ggcacccggc cggggagcac tcttcttcgc gctcggtgct ctcttctgcc tcgtcttctt
     2341 cgagagagag atagtcttcg ccggccttgt ggtgctctcg ctcctcgaca gcgtcaccac
     2401 cctcgccggc acccggttcg ggaagacccg gatctacaac cacaagtctc ttgaggggac
     2461 gctcgccggg gtcgcggtga cgacggtggc gctctgtctc ctcgtcccac cggcggtcgc
     2521 cctcgtgacg gccgcggccg cagccgtcgc cgagctggtg agccccgtgg acgacaacct
     2581 cgtcattccg gtcgtggcct gcctcgccct cacgctgctg ctctgagata tcgtgggccg
     2641 tgggtcaccg ctcttcttcc gatctctcat ggagcgccgg ccggtcgcac tccgggcagg
     2701 cggcaaagat tccgcaggcg attcaacgcg tgaggtcaaa acgcgccttt gtgatgaggg
     2761 cggcgacatc gggcacgacg atggggtcaa gcccggtaag ccggatcagg atgagcccga
     2821 tcacgacacc ggcggaggtg gagacgtcta tctggaggtg agaggacccg ccctcagtcg
     2881 accgtgagga tgatgagcga gtcggtcccc tcgcgcccgg gggatacccc ctcggtgagc
     2941 accacgcgct ccccgggacg gatgagaccc gaatcccgga ccgagtcgag tatcgcgccg
     3001 tgccagtact ccttttcgct ccggatcagg aacgggcaga ccccgtagga gaacgcgagg
     3061 aacctggaag tggccgggtt ccgggtgaac gcaaggatcc aggccccggg tttgaaccgc
     3121 gagatgtttc gcggagtgct cccggaacgg gtgggtgtca gcacgaaccg gatattgagc
     3181 gcatccagcg cctcgatgac gtttaaggag acgacatcgt tgacggtgat cctctcccga
     3241 cccttcccct cccggaagta gctgagaggg gcacacctgc ctccgacggc cgtgcgcttc
     3301 tcctcggtcg atcgggcgat ctttgccatc atcgcgaccg tctcgaccgg atacttcccg
     3361 atggacgtct cctcggagag catgaccgca tccgtcccgt cgaggatggc gttcgcgacg
     3421 tcggtcacct ccgcacgggt gggccggatg ttgtcggtca tcgacatcag catctgcgtg
     3481 gcggtgatca ccggccggcc gagtatgttg gccttctgga tgatccgctt ctgcaccgca
     3541 ggaacctcct cgatgggagt ctggacgccg aggtcgccgc gggcgatcat gaccccatcg
     3601 gtctcgccga ggatctcatc gaggtgatga agcgcctcct gtcgctcgat cttggctatc
     3661 acccggatag atttcccgga tccggccgca tacgcccggg ccttccggat atcctccgcc
     3721 cgctcgatga aggatatgct gaacgtgtca agcccctcgt tgagcccgaa gtcgatgcac
     3781 tcgaggtcgc ggtcggtcac ggcgctgagc gcaacgatcc ccccgccgcc gacgaggctc
     3841 atccctttgc gcgagagaag gggaccgccg atgaccacgg tcgcccggac atccttctcg
     3901 gcgacgttgt cgacccggag ctggaggaac ccgtcgttga ggaagacggt gctcccctcc
     3961 gagaccagct ggggaaactg tgggaactcg acgggtatcc gggaagggtc atctgatacc
     4021 ggggcggtcg tgagggtgac gacctcgccc ttctcgagtt ccaggggctc gtgcgcaagg
     4081 tcgccgatcc ggatcttcgg ccccggaaga tcgatcagga tcgtgatggg gagcccggtc
     4141 tcctcggccg cggcccggat gtttctgatg ttctcccggt gctcgtcaaa cgttccgtgg
     4201 gagaggttga gccgggcaac gttcatgccg gcccgggcca gcccggcgag cacctccggg
     4261 gaccgggatg tgggcccgat ggtacagacg atcttggtct tctggtcagg cagggtcaac
     4321 cgcttccgtt ttgcctgttc tgatatccgt tgcagcacct cttcgagcat cataaagtat
     4381 cttcaggtgc cgacaccgga ttaaccttcg cccggcaaga ccggcggacc cgggggaaag
     4441 aagaatacac gaaggtttcc ggccaattca ccggtggtgc ggccccgatc actcccgggc
     4501 ggcaaggtcg ttcgccggct cctgcaccgt ggtcaccctg atagccctcc cgctcccggc
     4561 gctgtactcc ccgatacgcc ggcgggcatg ctcgaccatc cccccggtgg tgatgatgag
     4621 cgagttctct ccgatgagag cctttaagat gcacttgtcg atgaccccgt aggtgtagtc
     4681 gaggaagatc tgttcccggc cggcgaactc cttcgcgcgg gtgcccatcg ccccgacgtg
     4741 cgcgaccgtg ccccgttcca cgagcgcccg gatatgggct gcgtcgatcc ggtcggcgtc
     4801 gcagacggag atcgccccgg gctcacaccg ggccggggcg cggccgtgca tcgcccgctc
     4861 gatccggtcg gtgaactcaa gcacgcactt tgggggggtg ccgggagcga cgatgcccaa
     4921 aagaccgagc tcgttgtaga ggtcaaggag agcgggcggc tttaagcggg cggccgcgag
     4981 gagatcgtgg tcagagaaga cctcctccgg cgacccccgg gcgaggatgc ttccccgctc
     5041 catgaggacg accgagtcgg cccagcggta ggcgagctcg acgtcgtgcg tggatatgag
     5101 caccgtccgc ccggaggagg caagctcgtc gaggagttcc atcacctcct cggaactcgc
     5161 gggatcgagg gtgttcgtcg gttcgtcgaa gacgaggacc gccggatcca tcgcgaggat
     5221 gccggcgatg gcgactcgtt tcttctcgcc gccggagagg tggtggggcg gccgcctctc
     5281 gtaaccgtca agtccgacgt aaccgagcgc gcctttgacc gcccggcgga cggcgtcgga
     5341 agagagaccg aggttcaccg gcccgaaggc cacgtcggcc tcgacggtcg gagcgatgat
     5401 ctggacgtcg gggttctgga agacgaaccc gacctgccgg cggagttccc ggaggctccg
     5461 gcggtcgtag gcgatcggtc ggccggagaa gcggacctcg cccgaggcgg gccggagcat
     5521 gccgttgagc atcaggagga gcgtcgactt tccggcgccg ttcgggccga cgagagcggt
     5581 ctttgaaccg gcagcgatcc ggatgctcac cccggcaagg gcggccgtgc cgtccgggta
     5641 ggtgtaggcg acgttgtcgg tctcaagcag tggtgtcgtc atatgagggc aattcctccg
     5701 gtggagagca agacgccgag aatgattgcg gtgtagagca gggccgcggc gagcgctagg
     5761 agggatatcg gccggagttc cccgggaata tcgagttttc catcataaca gcgtgcatcc
     5821 atcgcaagca cgagggcctc gccgccctcc caggtccgta tgaagagggc tcccgcgagc
     5881 atcccgaatg agttcaccga ctcgcggaac cggccgtacc cgaggcgcat cacctgcgaa
     5941 cggtagatct ggccggcctc ctcgatgagg atgaaaatga accggtaggt gagcatggcg
     6001 atatcgagaa ggacggtggg caccccgagc ctccggagta tatcgaagac ctcggtcatc
     6061 ggcgtcgtga gcgcgatgaa gtagagcgag cacatgccgc cgaagacccg ggcgaggagg
     6121 aggacggcaa ggtttgcgcc gccggccgtg acggcgaggg gaaggccggg aacctcgagg
     6181 aggaccgctc cgcctccggt gatgaagagg accacggcga tgctcatcag agcaaacgag
     6241 accgggatga ggaggaggcg ggcgtagacc cgggggggta tgcgcgcgag gaggaggacg
     6301 gcggcgctca tcgatgccgc gaccagcagg ggggcgacag ggccgggcga cgagacggag
     6361 aggaggatgc tcgcaagccc gacgaagagt ttgaggccgg ggtttacctg ccggagagcg
     6421 tttgtccggg caaagtcttc gagcacctcg tgcatatccc tgttcccctt accccgatcg
     6481 ccgcgagtca ggcggccttc ctgccgccct gccagtagcc gaatatgtac ccgatgacta
     6541 tcgcgccgat ggcggcctgc agggcaaaga ggagcgactc gatctcgccg ctcgggggtt
     6601 cccagaaggg ctccacccag ggctcgtagc ccgaagcctc gatgagatcg gcggcgttgc
     6661 tgtcggcccc gccccaggcc tcctctcccg aggcgcggat agcggcatcc tggaacagga
     6721 agacggcggc aaatatgacg atgagcgcgg cgaccgcgat ctccatgccg tacttcatgc
     6781 cggaacctcc cggagtttct ggatggtgct ctcggagagg aggtttaggc gggtgaggat
     6841 ctcgggtttg agctggacga tgtacttgaa gaccagcgca atgaccgcgc cctcgatgat
     6901 ggcgagcggg acctgggtgg cggcgaagat cgcggcaaag gtcaagaacg ccccgaagaa
     6961 cccggtggtc ccggggaacg cgagagcgag ctggaccgat gtgacgacgt aggtcgcaag
     7021 atcggcgagg gctgcggcga caaagaccgt cgcgtaagtg ttcgcgcccg cccttatccc
     7081 ggccctgtag acgatgtagg cgacggcggg tccggcgatc cccatcgaga agacgttcgc
     7141 cccgagggtg gtgagtccgc cgtgggcgag gaagaccgcc tggaagacaa ggacgatcag
     7201 accgaggacg gcggcgatgc aggggccaaa gagaatggcg ccgagtccgg tccccgtggg
     7261 gtgggaacag ctcccgttga acgaggggag cttgagcgaa gagaggacga agacgaacgc
     7321 gcctgcaacc gcaagaagcg gcaacgcttc ccgtttctcc tgtacgagcc tgtttacctg
     7381 gtagagcccg atgacgatga agggcaggga gacaaggaac catatctccc accagggtcc
     7441 tggtaagaat ccttccatga tgtgcatgat gatcacccgt aaagcgccgt gcaggcgcgt
     7501 cactcaaatc gatttgtgtt aatacaaatt aatcttccct atcgaggtta taccgggtca
     7561 tatttatccc ctgcgatctg caacactaca gagacgatat gtgctcgatt ccacggatta
     7621 tcgttgccgg aacccacagc ggctgcggca agaccacgct tgcaagcgga ctgatggcgg
     7681 cgctcaccgc ccgggggctc gctgtccagc ccttcaaggt gggcccggac ttcatcgatc
     7741 ccacgcatca ctcggccatc tgcggccgga cgtcgcgtaa cctcgacccc ttcatgatgg
     7801 gggaggcggg cgtgcgggag acgttcgtcc ggacctcggc cggggccgac gtcgccgtcg
     7861 tcgagggtgc gatggggctc tacgatgggc ttgaggggag cgataccgcg agcacggccc
     7921 acgtcgcaaa gatcctcgat gctccggtca ttctcgtggt ggacgccggg ggcgcgtcgc
     7981 ggagcgtgca tgcgatggcc ctcggctacg cggggttcga ccctggcgtc cgggtcgccg
     8041 gcgtcatcct caaccagatc gggagcgccc gccaccgggc catgatcgag gagacaaaga
     8101 gccttcccat ctatggagga gtcccccggc gaaaggacct tgcggtcgag agccggcacc
     8161 tcggtcttgc gatggcggct gaggatgatg cgatggcgca gttcggtgcg gtgatggagg
     8221 agacatgcga tctctcccgg atcgtggatc tcgcgcggtc tgccccgtcc cttccggcgc
     8281 cggcagaggg cgggagccgg tcggagaccc gggtgcggat cggtgtcgcg caggatgcgg
     8341 cattctgttt ctactatgcc gataacctcg accggctcgc acgggccgga gcggacctgg
     8401 tcttcttctc gacggtggcg gaccgactcc cggatgtgga tgccctctac ctcggcggcg
     8461 gctacccgga actccacgcg gcggcgctcg cggcttcccg gtgccgggag gatatccgcc
     8521 gggctgccga cgacggcatg ccgatctacg cggagtgcgg cgggctcatc taccttgcgg
     8581 aacagctcgc gaccgacggg ggcgactacc cgatggcagg gatcctcccc gcggcggccg
     8641 tgatgacgga ccggatccag gctctcggct acgtggaggc ccgggtggcc ggcgggacac
     8701 cggtccttat accgggttcg gccttccggg gccacgagtt ccactactcg cgcctcgact
     8761 gcgcccccga cgcgaggttc gccctgaccc tcctccgggg aaaggggatc gacggcggcc
     8821 gggacggact tatcgcgcag aacgcggtcg ggcaatatac ccacgcttac ttcacggagg
     8881 ggtttgccgg gatgctggtg caggctgctc tggcataccg gcggtcgtag agcaaaaaaa
     8941 ggggtcagga ggtcgtgttg ccggtgacga cgatgagccc ggtaaacccg gttggcagtg
     9001 acatgtagga gtagttgtcc ggcgtggcga aggtgaagtt atacgagtcg cccggctgaa
     9061 gtacccccga gtcgaagtcc gccgcctgac cggtcgccgt gacggtctcg ttttcggcgg
     9121 cgttgttggt ccaggtcacc gttgtgccct gggagacggt gagcacgtcc ggcacgaacc
     9181 ctctatcggt gatggagacg ctctcgccgc cggctgccgg cgg
//
LOCUS       DAOSEE010000086         9150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_2818_length_9150_cov_230.759758, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000086 DAOSEE010000000
VERSION     DAOSEE010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 9150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9150
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2818_length_9150_cov_230.759758"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2375)
                     /locus_tag="PLI76_07495"
     CDS             complement(<1..2375)
                     /locus_tag="PLI76_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HOI61913.1"
                     /translation="MPEIYLKVDSAYPEDQGAGKARLDPDTMLQLRLSPGDLVAIEGK
                     RRTVAKVWRAMVNDWHQGKVRIDNFTRLNTGASIGDRIKIKTLDEEVEAKRVVLAPPE
                     DLPKQLPINYSSVVNKLIDFPVVKNDSVPIQAGLPFMQPQLVAFKAVVVEPENAVIIT
                     KNTKIEFSEKPAAGFEGVKRISYEDIGGLKGELQRVRETIELPMRHPEIFHKLGIEPP
                     KGVLLYGPPGTGKTLIAKAVASESGAHFISIAGPEVISKYYGESEQRLREVFEDARQH
                     APAIIFIDELDSIAPRREEVTGEVERRVVAQLLTMMDGLEERGQVVVIGATNRLDAID
                     PALRRPGRFDREIEIGVPPEGDRAQVLHIHTRGMPLGEGVDIAAIAQQTHGFVGADLA
                     ALAREAAIKALRRYLPEIDLEAEEIPAETLEKMEVQGKDFRDALRDVGPSAMREILLE
                     VPHTTWGDVGGLEEAKQDIREAVEYPLTRREQFEDLGIEPPKGVLLYGPPGTGKTLIA
                     KAVASESGANFVPVKGPQLLSKWVGESERAVREVFKKARQVAPSIIFFDELDALAPAR
                     GGGSESHVIESVLNQILTEIDGLEELRGVVVMGATNRPDMVDPALLRPGRFDRLVYIG
                     EPGRDDREKILRIHTRYMPIEGSTIEDVVDMTEGLSENAVEDLMLAVDAGRHTSIEDL
                     RSRRAMIEADGDEGLRRSLRRRKIVDLLSQQKIALDDPARSRLIRKIATETEGFVGSD
                     LEALGREAAMLAMREGASHVNATHFDRAQEKVHATMNERLRQYYGRVQQHF"
     gene            2422..3615
                     /gene="larC"
                     /locus_tag="PLI76_07500"
     CDS             2422..3615
                     /gene="larC"
                     /locus_tag="PLI76_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HOI61914.1"
                     /translation="MNVLLFDPFNGAAGDMIIGSLLDLGADEGVVRAAMRSVVGEPTI
                     ERVDRSGIRAVRVRAHAPVDHRTLEEVLDRVAESDTPAPAREMARRVFLRIHRAEESI
                     HGHGAHFHEVGADDAIADVVGACTALYSLAPDGVAVLPVALGRGFAVGAHGTFPIPAP
                     ATVAILAASDLRTVPGGEERELCTPTGAALLAEFSTTRPDDIGPAAIRAVGYGAGSRN
                     PPGRPNVLRSMIFATDEKAGGDRVDILETNVDDVTGETLAYTINRLMEEGARDASATP
                     VVMKKGRSGHLIRVISYPDATGRLMGVMAAELGTLGIRCIPMIHRAIAERTVEQITVT
                     ISGREFAIDVKCGWSGGEITSFKAEHEQAAACARDTGIPLREVTGTVEAAARRLFIER
                     GVLAS"
     gene            3612..4289
                     /gene="radB"
                     /locus_tag="PLI76_07505"
     CDS             3612..4289
                     /gene="radB"
                     /locus_tag="PLI76_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair and recombination protein RadB"
                     /protein_id="HOI61915.1"
                     /translation="MKPDRVSTGSRPLDDLLGGGLERRTITQIYGEPASGKSTFCLMA
                     AVASLRAGNSVIYIDTEGFSVERFAQVAGGHAAEFADRLYLFEPIDFAQQGAMIADTE
                     TLLRKKDGAPVDLLVMDSMTALYRTELDLGREAVRRLSHHVIKLLGLAKKYDIPVLIT
                     NQIYVDVERDRVSGLGGTALEHISKAIVRLEKRDSVRRAMLRKHRSRPEGISFDFVIT
                     EDGIRAV"
     gene            complement(4276..5094)
                     /gene="dapF"
                     /locus_tag="PLI76_07510"
     CDS             complement(4276..5094)
                     /gene="dapF"
                     /locus_tag="PLI76_07510"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOI61916.1"
                     /translation="MEIAFTKLQGNGNDFILIDEYQHEIIPEEMKGSFAALYCDRRFG
                     IGADGVLFLQASNHADVRMRLVQPDESEAAMCGNGIRCLAKYAYDAGYAGESCSVETG
                     AGVVPVAMGYVDGEFAATITMPEPAFERSAIPATGNGEYREDIRGFAVHAVNTGVPHA
                     VVFAEEVDAIDLAAVGPVIRNHPTFPEGANVNFVECAGNDILRVRTFERGVEAETECC
                     GTGATASAAVARRLGYVGETVRVETNGGPLVISFSKAVTMEGPAETVFFGVIPL"
     gene            5230..6879
                     /locus_tag="PLI76_07515"
     CDS             5230..6879
                     /locus_tag="PLI76_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOI61917.1"
                     /translation="MPSGEPHWRKSLSADIVAGTTTALVGIPQTMGFALVAGINPIYG
                     LYTAAFSTAFGALLTGSSFLKVMLSNVIAVSLFSILAPVPESDVPATLFVLTLLVGLF
                     QLGFGLARAGSLTRFISNAVLTGFIIGAALLIVLGQLGNLTGYELPGDAIQILAVLDL
                     ILHAGDIQVQPLAVGLLTIALALTFRRAPRLSSAALLLPIVITTLLVRAADLEVALVG
                     DISAIPAGLPFPVLPELALAPGLLVPALALAIIGLVMAIGITEKTPEPDGTIADVNRD
                     FAGQGVANILASFFQCAPSSGSLSATTLNVSAGAETRAANLISGILVGAVILVAGPLA
                     ELIPLSALAGILILIGAELIYRPHEIVCICRYSRPGRWGMIATFISTQVLPLQYSIYV
                     GVFLSLAIYLITSTREASVVRLVPVEGGMFREESPPGWLPDGRLTILSIAGSVYFATL
                     RAIERSLPSPEGTDGAVVILALRGRVEAGTGFFRMLERYARRLLAHNGRLILAEVDPR
                     LLAGLAETGVTETVGPANIFVATPTIWESLMEAIQTAEAGT"
     gene            6934..7956
                     /locus_tag="PLI76_07520"
     CDS             6934..7956
                     /locus_tag="PLI76_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOI61918.1"
                     /translation="MNPESLSPPARIAIVGAAVVIVLAGIQAATPILGPFLIAVFFAM
                     ITAPIMAWLTRRGVPRALAAGMVVTGLAVVLVGTIAFLGMAFTEFLLSLPRYEKALEA
                     QATIIASYGIDTTGIQVWDYIDRGFVIQQAAEIARQVGNIAFDAIIVAIAIGFLLIEA
                     PRFAAALARRLGPESPPYRHLTQSGRFLIEYVVIRTEVNLVTGVGTGLFLALLGVEFA
                     ALWGFIAFILSYVPYIGLVVAAIPPTLLALVGFGPSGALAVIVAITLIDAAAENLVFP
                     QITRKGLNLSPFVVIFSVVFWGLVLGAVGVFLAIPLTIAVKLFLESWDETRWLGEMMN
                     AGERQK"
     gene            8089..8490
                     /locus_tag="PLI76_07525"
     CDS             8089..8490
                     /locus_tag="PLI76_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HOI61919.1"
                     /translation="MLERMETGAGDIAGFRFDGDMTTKDYNGVLIPALEEAGGTHPVF
                     RILFSIVNFHGWKPRGNWEALKDWPGMEKVDRIAIVGGEKWEEWMNYLPGLFVGFVGI
                     DVRYFSSEHLNDALDWLREPIAAEEAALKPG"
     gene            8580..>9150
                     /locus_tag="PLI76_07530"
     CDS             8580..>9150
                     /locus_tag="PLI76_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOI61920.1"
                     /translation="MRHYKDIIFEKPLPEAIRRLGLLPADMHFHTRHSDSPTRVRDAL
                     KLAARRGIGLAITDHNQVGGVVEAGRQNIRVPLVPGVEVSANDGPHILLYFYSVADLL
                     DFYRCHIEKNRRNGPFTAIRLDTQEILDHREDYACIAAEAHPCGYIFLNRGVQRCVAG
                     RCIDREVFSRLDALEVICGGMARSHNLKAA"
BASE COUNT         1500 a         3131 c         2837 g         1682 t
ORIGIN      
        1 ttgaagtgct gctgcaccct cccgtagtac tgccgcagcc gttcgttcat cgtcgcgtgc
       61 accttctcct gcgcccggtc aaagtgcgtc gcgttcacgt gggatgcacc ctcccgcatc
      121 gcgagcatgg ccgcctcccg gccgagcgct tcgaggtccg agccgacgaa tccttcggtc
      181 tcggtcgcta tctttctgat gagccggctt cgcgcgggat cgtcgagggc gatcttctgc
      241 tgggagagga ggtcgacgat cttcctccgc cggagagagc gccggagccc ctcatcacca
      301 tctgcctcga tcattgcgcg gcggctccgg aggtcttcga tactcgtatg gcggccggcg
      361 tcgaccgcga gcatcaggtc ctcgaccgca ttctccgaga gcccctcggt catatccacg
      421 acatcctcga tcgtagaacc ctcgatcggc atgtagcggg tatggatcct gaggatcttc
      481 tcacggtcgt cccgcccggg ctcgcctatg tagacgagcc ggtcaaaccg cccgggccgg
      541 aggagcgccg ggtcgaccat atccggccgg ttggtcgcac ccatcaccac gacgccgcgg
      601 agttcctcaa ggccgtcgat ctcggtgagg atctggttta gcacgctctc gatgacgtgc
      661 gactcgctgc cgccgccgcg ggcgggggca agggcatcga gctcatcgaa gaatatgatg
      721 gacggcgcta cctgccgggc cttcttgaag acctcgcgga cggcccgctc gctttcgccg
      781 acccacttcg agaggagctg agggcccttg acgggcacga agttcgcccc gctctcgctt
      841 gcgaccgcct ttgctatcag ggtcttaccg gtcccgggcg gcccgtagag gaggacaccc
      901 ttcggaggct cgatgccgag atcctcgaac tgctcccgcc gggtgagcgg gtactcgacc
      961 gcctcgcgga tgtcctgctt tgcctcctca agcccgccga catcccccca ggtggtgtgc
     1021 ggcacctcga ggaggatctc ccgcatggcg ctcgggccca cgtcgcggag ggcgtcgcgg
     1081 aagtccttgc cctgcacctc catcttctcg agggtctctg ccggaatctc ctcggcctcg
     1141 aggtcgatct cggggaggta gcgccgaagc gccttgatag ccgcctcacg ggcaagagcg
     1201 gcaaggtccg cccccacaaa cccgtgggtc tgctgggcga tggcggcgat atccacgcct
     1261 tctccgagcg gcatgccccg ggtgtggatg tggagcacct gggctcggtc gccctccggc
     1321 gggacaccta tctcgatctc ccggtcgaac cggccggggc ggcggagagc cgggtcgatg
     1381 gcgtcgagcc ggttcgtggc cccgatcacc acgacctgcc cccgctcctc aagcccgtcc
     1441 atcatcgtca ggagctgggc aaccacacgc cgctcgacct ccccggtcac ctcttcccgc
     1501 cggggagcga tcgagtcgag ctcgtcgatg aatatgatgg ccggcgcatg ctgccgggca
     1561 tcctcgaaga cctcgcggag gcgctgctcg ctctcgccgt agtacttcga tatcacctcg
     1621 ggccccgcga tggagatgaa atgggcgccg ctctcgcttg cgaccgcctt tgctatcagg
     1681 gtcttacccg ttcccggcgg gccgtagagg aggacgccct tcgggggctc gatgccgagc
     1741 ttgtggaata tctccgggtg gcgcatggga agctcgatcg tctcgcggac acgctgcagc
     1801 tcgcccttca ggccgccgat gtcctcgtag ctgatcctct tgacgccctc aaacccggcg
     1861 gcgggttttt cggagaactc gatcttcgtg ttcttggtga tgatcacggc gttctccggc
     1921 tcgaccacca cggccttgaa cgcgacgagc tggggctgca tgaacgggag ccccgcctgg
     1981 atcggcacgg agtcgttctt cacgacggga aagtcgatca gcttattgac gacactgctg
     2041 tagttgatgg ggagttgttt cgggagatcc tcgggaggcg cgagcaccac tcgtttcgcc
     2101 tcgacctcct catccagcgt ctttatcttt atacgatccc cgatactggc gccggtgttg
     2161 agccgggtaa aattatcgat tcggaccttt ccctggtgcc agtcgttcac catggcacgc
     2221 cagaccttgg ccacggtgcg gcgcttaccc tcgatagcga cgaggtctcc ggggctgagc
     2281 cgaagctgca gcatggtgtc agggtcaagc cgtgccttgc ctgccccctg atcctccggg
     2341 tacgcgctat ctactttcaa gtatatctcg ggcattacct ctaattctta tattcggggg
     2401 atttataagt actggaggcg catgaacgta cttctcttcg atccgttcaa cggggccgca
     2461 ggcgacatga tcatcggctc gcttctcgat ctgggggcgg acgagggggt tgtccgggca
     2521 gccatgcgct ctgtcgtcgg cgaaccgaca atagagcggg tagaccgttc cgggatccgc
     2581 gcagtccggg tgagagcgca tgcaccggtc gaccaccgta cgcttgagga ggtgctcgac
     2641 cgggtggccg agagcgacac ccccgcccct gcacgggaga tggcccggcg ggtctttctc
     2701 cggatacacc gggctgaaga gagcatccac ggacacgggg cacacttcca cgaggtgggt
     2761 gcggacgacg ccatagccga tgttgtgggg gcctgcaccg ccctgtactc cctcgccccc
     2821 gacggtgtcg ccgtcctccc ggtcgccctc ggccgtgggt ttgccgtcgg ggcccacggc
     2881 accttcccta tcccggcccc cgcgaccgtc gctatccttg ccgcatcgga cctccggacc
     2941 gttcccggcg gcgaggagcg agaactctgc accccgacgg gggccgctct ccttgccgag
     3001 ttctcgacga cccggcccga cgatatcggg ccggcggcga tcagggcggt cggctacggg
     3061 gccgggagca ggaacccgcc gggccggccg aacgtcctcc gctcgatgat cttcgcaacg
     3121 gatgagaagg ccggcggcga ccgggtcgat atcctcgaga cgaacgtcga cgacgtcacc
     3181 ggggagaccc ttgcctacac gataaaccgg ctgatggaag aaggagcacg ggacgcaagc
     3241 gccactcccg tcgttatgaa gaaggggcgg agcggccatc tcatccgggt catctcctac
     3301 cccgatgcga ccggccgcct catgggggtg atggccgcag aactcgggac gcttggcata
     3361 cgctgcatac cgatgatcca ccgggcgatc gccgaacgga cggtcgaaca gatcacggtg
     3421 accatcagcg gccgggagtt cgctatcgac gtcaaatgcg ggtggtcggg aggggagatc
     3481 acctcgttca aagcggagca cgagcaggcg gcggcgtgtg cccgggacac gggcatcccc
     3541 ctccgcgagg ttaccggcac cgtcgaggcg gcggcacgtc gcctcttcat cgagcggggc
     3601 gtgctcgcgt catgaagccg gaccgggtca gcacgggaag ccggcccctc gacgacctgc
     3661 tgggcggggg actcgagcgg cggacgatca cccagatcta cggcgaaccc gcgagcggca
     3721 agagcacctt ctgcctgatg gcggcggttg cgagcctccg ggcggggaac tcggtcatct
     3781 atatcgatac ggaaggattc tcggtggagc ggttcgccca ggtcgccggg gggcatgccg
     3841 cggagttcgc cgatcggctc tacctctttg agccgatcga tttcgcccag cagggggcga
     3901 tgatcgccga tacggagacg ctgctcagga agaaagacgg cgcacccgtc gacctcctgg
     3961 tgatggattc gatgaccgcc ctctaccgga cggagctcga cctcgggcgg gaggcggtgc
     4021 ggagactctc gcaccacgtg ataaaactcc tcgggcttgc aaagaaatac gacatccccg
     4081 tcctgatcac gaaccagatc tacgtggacg tggagcggga ccgtgtctcc ggcctcgggg
     4141 ggacggcgct tgagcatata tccaaggcca tcgtgcgact cgaaaagagg gatagcgtcc
     4201 ggcgggccat gctccggaag caccggtccc ggccggaagg gatttcgttt gatttcgtga
     4261 tcaccgaaga cgggatcaga gcggtataac accgaagaag accgtctcgg cggggccttc
     4321 catcgtgacg gctttgctga agctgatgac gagcgggccg ccgttcgtct cgaccctcac
     4381 cgtctccccg acgtagccga ggcggcgggc gaccgccgcg gaggccgtgg cgccggtgcc
     4441 gcagcactct gtctcggcct cgacgccgcg ctcgaatgtc cgaacccgga ggatatcgtt
     4501 tccggcgcac tcgacgaagt tgacgtttgc gccttcggga aaggtgggat ggttccggat
     4561 gaccgggccg acggcagcga gatcgatggc atcgacctct tctgcaaaga cgacggcgtg
     4621 gggaacgccg gtgttcacgg cgtggacggc aaacccgcgg atatcctccc ggtactcccc
     4681 attcccggta gccggtatgg cgcttcgctc gaacgcaggc tccggcatcg tgatcgtcgc
     4741 ggcgaactcg ccgtccacat agcccatcgc caccgggacg actccggccc cggtctcgac
     4801 cgagcaggac tctccggcgt agccggcgtc ataagcgtac ttcgcgaggc accggatgcc
     4861 gttcccgcac atggcggcct cgctttcgtc gggctgcacg agccgcatcc ggacatcggc
     4921 gtgattggac gcctggagaa agaggacgcc gtcggcaccg ataccgaacc tgcgatcgca
     4981 gtaaagcgcc gcaaacgatc ccttcatctc ttcgggaatg atctcgtgct ggtactcgtc
     5041 gatcaggata aagtcattgc cgtttccctg cagtttggtg aacgcgatct ccatggatag
     5101 tagcctctct atgataggta ttgggagcgt cgagagataa accgtggtgc cttcaccgtc
     5161 gttcctccac ccttgccggg caagcccgaa gagtaactat atacccggcg gcccgcactc
     5221 tcccggcgca tgccctcggg tgaaccgcac tggaggaaga gtctctccgc cgacatcgtc
     5281 gccgggacga ccactgccct ggtcggcatc ccgcagacga tggggtttgc cctcgtcgcc
     5341 ggtatcaacc cgatctacgg cctctacacc gccgcgttct cgacggcgtt cggggcgctc
     5401 ctcaccggct cctcgttcct caaggtgatg ctctcaaacg tcatcgcggt ctcgctcttc
     5461 tccatcctcg ctccggtccc ggagagcgat gtcccggcca ccctttttgt cctgacgctc
     5521 ctcgtcggac tcttccagct cgggttcggt ctcgccaggg ccggcagcct gacccggttc
     5581 atatcgaacg ccgtcctcac cgggttcatc atcggagcgg cactcctgat cgtcctcggg
     5641 cagctcggga acctcaccgg ctacgaactc ccaggggacg cgatccagat ccttgccgtc
     5701 ctcgacctca tactgcatgc gggcgatatc caggtgcaac cgcttgccgt cggcctcctc
     5761 accatcgccc tcgccctcac ctttcggcgg gcgccgcgcc tcagttccgc cgccctcctc
     5821 ctcccgatcg tcataacgac cctccttgta cgggcagcgg atctcgaggt cgcgctcgtc
     5881 ggcgatatca gcgccatccc cgccggcctc ccgttccccg tcctccccga gctcgccctc
     5941 gctcccggtc tcctcgtccc ggcgcttgcc ctcgccatca tcggtctcgt gatggccatc
     6001 gggatcaccg agaagactcc cgagcccgac gggaccatcg ccgacgtcaa ccgtgacttt
     6061 gccgggcagg gggtggcgaa catactcgca agcttcttcc agtgtgcacc ctcaagcggc
     6121 tcgctctcgg cgacgacctt aaacgtcagc gcgggggcag agacccgggc ggcaaacctc
     6181 atctcgggca tcctcgtcgg ggcggtcatc ctcgtcgcgg gcccgctcgc ggaactgata
     6241 ccgctttcgg ccctcgccgg catcctgatc ctcatcgggg ccgaactcat ctaccggccc
     6301 cacgagatcg tctgcatctg caggtactcc cgccccgggc gctgggggat gatcgccacc
     6361 ttcatatcca cccaggtcct gccgctccag tacagcattt acgtcggcgt ctttctctcg
     6421 cttgccatct acctcatcac atccacccgc gaggcctccg tggtccgcct cgtcccggtg
     6481 gaggggggaa tgttccggga agagtcgcca cccggctggc tccccgacgg ccgcctcacc
     6541 atcctctcga tagcgggaag cgtctacttc gccaccctcc gggccatcga gcggtcgctc
     6601 ccgtcaccgg agggaacgga cggcgcagtc gtgatcctcg ccctccgggg gagagtcgag
     6661 gccgggacgg ggtttttccg gatgctggag cgctatgccc ggcgccttct cgcccacaac
     6721 ggccggctca tcctcgccga ggtcgatccc cggttgcttg cgggcctcgc cgagaccgga
     6781 gtaacggaga ccgtcggtcc cgcaaacata ttcgtcgcga ccccgaccat ctgggagtcg
     6841 ctcatggagg cgatccaaac ggcggaggcc ggaacatgag accccgcccg gaaaactctt
     6901 tctttcccgg gcacccatac ctttcttgtg gatatgaacc ccgagagcct ctctcccccc
     6961 gcccgtatcg cgatcgtcgg ggcggcggtc gtcatcgtcc tcgcggggat ccaggcggcc
     7021 acaccgatcc tcggcccatt cctcatcgcc gtcttctttg ccatgatcac cgcgcccatc
     7081 atggcatggc tgacccgccg gggagtgccc cgggccctcg ctgccgggat ggtggtcacg
     7141 gggctcgccg tggtccttgt cgggacgatc gccttcctcg gcatggcctt caccgagttc
     7201 ctcctctccc tgccgcgcta tgagaaagcg ctcgaggcgc aggcaaccat catcgccagc
     7261 tacggcatcg atactaccgg catccaggtc tgggactaca tcgacagagg atttgtgatc
     7321 cagcaggcgg cagagatcgc ccggcaggtc gggaacatcg ccttcgatgc catcatcgtc
     7381 gcgatagcga tcgggttcct cctcatcgag gcgccccggt tcgcggccgc ccttgcacga
     7441 cgcctggggc cggagagtcc accctaccgg cacctcacgc aatcgggccg gttcctcatc
     7501 gagtacgtcg tgatcaggac cgaggtgaac ctggtcaccg gggtcgggac cgggctcttc
     7561 cttgccctcc tcggggtcga gttcgcggcg ctctgggggt tcatcgcctt catcctgagt
     7621 tacgtcccct acataggcct cgtcgtggcc gcaatacccc cgacactcct tgccctggtc
     7681 gggttcggtc cgtcgggggc cctggccgtc atcgtcgcca tcaccctcat cgacgccgcg
     7741 gcggagaacc tggtcttccc ccagatcacg agaaaggggc tcaacctctc gccgttcgtc
     7801 gtcatcttct cggtcgtctt ctggggtctc gtcctcgggg cggtcggggt cttcctcgcc
     7861 atacccctga cgatcgcggt gaaactcttc cttgaaagct gggatgagac ccggtggctc
     7921 ggggagatga tgaacgccgg ggagcggcaa aagtaacatc ccccgggtcc ggccacgggc
     7981 gctccatccc tgcaatatcc cgcgccgggg ctcctcccgc tcgcgccagc cccggaccgg
     8041 gggagcgacc ggaacctttt tctggcgtgc cccggaaggg ggggcgccat gcttgagcgg
     8101 atggagacgg gtgcaggaga catcgccgga ttccgattcg acggtgatat gacgaccaaa
     8161 gactacaacg gggtgctgat accggcgctc gaagaggcgg gaggaaccca cccggtcttc
     8221 cggatcctct tctccatcgt gaactttcac ggctggaaac cccgcggaaa ctgggaggcc
     8281 ctcaaggact ggccggggat ggagaaggtc gaccggatag cgatcgtcgg cggtgagaaa
     8341 tgggaggagt ggatgaacta tctaccgggg ctctttgtcg ggtttgtcgg catcgacgtc
     8401 cgatacttct caagcgaaca cctgaacgat gccctcgact ggctgcggga gccgatcgcc
     8461 gcagaagagg ccgctctcaa gccgggatag cgctgccaaa acgagagatg cgagatcccg
     8521 ggagagcagg caaccttttt tcatccccgg caacctaccg gtgatcagac agcgatccca
     8581 tgcgccatta caaggacatc atattcgaaa aacccctgcc ggaggcgatc cggcggctcg
     8641 gtctcctccc ggctgatatg cacttccata cccgccactc cgattcgcca acccgcgtgc
     8701 gggatgcact caaactcgcc gcccggcggg ggatcgggct tgcgatcacc gaccacaacc
     8761 aggtcggcgg cgtcgtcgag gccgggcggc agaatatacg cgttcccctc gtacccggtg
     8821 tcgaggtcag cgccaacgac ggcccgcaca tcctcctcta cttctactca gttgccgacc
     8881 tcctcgactt ctaccggtgc cacatcgaaa agaaccgaag aaacggcccg tttaccgcga
     8941 tacgcctcga tacccaggag atcctcgacc accgggaaga ttacgcctgc atcgccgccg
     9001 aggcgcaccc ctgcggctac atcttcctga accggggggt ccagcggtgc gtcgccggcc
     9061 ggtgcatcga cagggaggtc ttctcccgcc tcgacgccct cgaggtgatc tgcggtggta
     9121 tggcccggtc ccataacctg aaggcagccg
//
LOCUS       DAOSEE010000087         9034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_2864_length_9034_cov_208.536363, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000087 DAOSEE010000000
VERSION     DAOSEE010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 9034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9034
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2864_length_9034_cov_208.536363"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PLI76_07535"
     CDS             <1..240
                     /locus_tag="PLI76_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOI61921.1"
                     /translation="DGEPVITIMLPDEDWETIGCGRHSAGDRMLTVDDVASTLNFTPG
                     RIREFIREERIPAVKCGGSWRIKRSELERIMNEGF"
     gene            322..4491
                     /locus_tag="PLI76_07540"
     CDS             322..4491
                     /locus_tag="PLI76_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOI61922.1"
                     /translation="MCSGASRELPNASLIDPVAGLDDGAAERTYCADGCAPGPGRDCG
                     RRSCILLSLFFCVALLALLFSTPADLWTPPAIEPLSPIVGLPGIFGPDARSFFKRWRG
                     RRAGEEEAPPDPAPVEHSGSEAGAPVKLEEAPANSLQLDDPVLPDSADAAGKTPEEFF
                     WFDDPLLPDGADALPVEQEPDDCSGEAGELPEPPPRSEPLTDAELMRLLGLDDEDDEP
                     SSGTPGYRAFPAEDEPTFAPGEDRPVESLIDDLGSPDADIRRRAVDAIREHGADAVAP
                     LVEALARADDGRRWCVAEALAAIGDDAIPALIAALGDDAVQTGAAATLVRIGEPAVPP
                     LIAALAGDDDEVQFGAFYALREIGDTAIPFLVGALDAPDGGARRSAARLLRELGWEPP
                     DDPGAIRYLIASEAWLDVAEYGGAAVGPLIAILRSPDREARWSAARTLGEIGKTAVGP
                     LVDLLADADDDIRWLAVMALAEIGSPAVGSLIRLLSDPSLCGTATAALLRIGEPAAEE
                     CVHALDDADGDALEALQEVLSALGEAAVPPLIQALTASRSRTRGHAAEILDGMGWEPW
                     NDAERAWYLIAREQWIELALMGEAAVVPLIRTLNGDDDHVRSEAAATLGEIGHPAAVL
                     PLVDVLANDAVAPAAADALVAIGRPAVDPVLALLEGGIGTVREHAVEILGRLGAHEAV
                     PTIVDLVRSGGDRLHRKAVDALVGIGAPAVGALIPLLGEDGDGYAGAEAALTGIGDAA
                     REPLIKALGDEDARTRMGAAMVLRRVGRAPAGVEGEAAYLIALQQWPEVVGLGAPAVD
                     LLAARLGDRDAGVRAGAAESLARLGAVAAPHLVSLLGNEARRGAAGETLVRIGEAAVP
                     HLIRALGEEALRRPAAGVLVRIGNPAAGPLVRVLGDPDTGQVAAKILGAMGESSIGAL
                     VGALGDDDIQVRQGAGGILIGLGDAGIGALIGALGHPDDAVRLEAVDMLIRIGRSAVA
                     DLTGALLDERYRVRLGAAEALGRIGWAPETEQETIGYLIAKEQWVSVAEFGADAVIPL
                     IRTLNDSDTAVQMGAARALGMIGAPAVTRLLGELRDGQEDGRRKAVEALKMVGEAAVN
                     PLIDALQDRDWHTRLGAARALVGIGNPAIEPLIRALRDGSPGIKMGAAATLGKIGNPA
                     AIGPLTDTLLHEDWRVGRVVVRALGMMGEPAVEALLGVLEKGDDAARKGAVAALVLIG
                     EPAKRLLPGALAAGHFRIRAGAADALDRLGWSPGPGEEMALYLIAKERWSDLVRLGPL
                     AVEPLVQVLDDHDDSVRRRAAKALGEVRDPRAVPALMALLHDDFYSIRREAAAALVAV
                     GPPAMGPVISALGDPDGDVRKRAADVLAGIGDARAVEPLRSIERDEDWYVRKAAEEAV
                     EKIRGRIGESV"
     gene            complement(4513..6000)
                     /locus_tag="PLI76_07545"
     CDS             complement(4513..6000)
                     /locus_tag="PLI76_07545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOI61923.1"
                     /translation="MTPAISPGGRKTLPSPLAQGDGILIVDADQVVVQANIQVQALLC
                     ATREEILGADAPDVVQRYLLPLLPEGESPGEVVALLCDGSYPQPVTLDVRVPGTGIRR
                     ISVTANTVDAVTPALRLLVFHDTGKGGAGRAEERPPPATERLIVVPGRERRTPEQEVP
                     GSGDVTTAARRSVADTAVLEQIAEDEDRFRSIFATSHAVMLIVDPQTGAIVDANPAAS
                     AYYGHPLETLTTMQIADLNVLDREDLFRDMQKAVTGEKRQFSFLHRLADGRYRDVDVF
                     SGRVIICGRVLLHSIIHDVTGRRRAEEQFRALLDATPDAAMLINHSGIILALNEVMAT
                     RFKKTVEELLGACAYDILTPELAMRRRELTIQALATGKPLRHVDEREGRILENVLFPV
                     PGDQEDSRRVAVISHDITRERELEQARQEAFDRIRQNIEQFAILGDHIRQPLQVILGT
                     ACLLEDEQATGKIQREIERINGYIRQLDQGWIDSRKIREYLQRHE"
     gene            complement(6107..6277)
                     /locus_tag="PLI76_07550"
     CDS             complement(6107..6277)
                     /locus_tag="PLI76_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61924.1"
                     /translation="MKRDRKGEGKAETEEYWCEICGAACDEITEETFPDLRKARTLCP
                     GCRKSYEDSCRL"
     gene            complement(6357..7181)
                     /locus_tag="PLI76_07555"
     CDS             complement(6357..7181)
                     /locus_tag="PLI76_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOI61925.1"
                     /translation="MAKKTILITGSTDGIGRAAAYLLAEQGHRILIHGRDRAKGRAVL
                     EEVEAAAVPEQPALFIADLSVQDRVRDLAEEVAGACDRLDVLINNAGVFMPERYVAPG
                     GIEMTFAVNYLAGLLLTHGLLPLLSKSAPARVVNVASIAHRSVRAVDWENIPAFEDYD
                     AYGAYATSKLGVAACTARLASVLDGTGVTANSLHPGVIDTKLLRAYTGGRDGGAPPEK
                     GAAVEAHLATSPDAGEVSGRYFEATRWTRPSPLALDADVQERFWEMGLDLVGVDRW"
     gene            7500..>9034
                     /locus_tag="PLI76_07560"
     CDS             7500..>9034
                     /locus_tag="PLI76_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOI61926.1"
                     /translation="MVRYSVTMDDDLVKTIDRMCDSLGMSRSEWLNKLCTAHLSGAPG
                     NGGGQGVAPTLMPPLQAPGPNMTDYETACASFKIDVPDYFNFGYDVIDRWAETDRNKL
                     AMIWVDQQGAEKKYSFRDLRNLSNGAANILLKYGVKKGDRVMLILPRIPEWWIFVIAL
                     IKLGAIFCPCPTMLTPKDIRYRIRAGKFRMIITNTENAEKIDEVADACPLLETLFLVD
                     GERDGWASYLHELDYPAPVSRHTVSMPVAKRTKSTDPMMIYFTSGTTGEPKMVLHNQG
                     YPLGHIITASLWQDLTPNDLHLTFSDTGWAKCAWGKIFGQWIAGACIFVYDIQGKFGA
                     TELLPLIEKYEVTTFCAPPTVYRMLILADLDKFDFRNLRHCCSAGEPLNPEVIRVWQD
                     GTGQPIYEGYGQTETVCCIATFPCMEHKPGSMGRPVPGWHIELHDDDGNPVGAGEEGK
                     IAVSLNPRPVGLFMEYLDNPEANRESFQNGFYYTGDKARMDEDGYLWFIGRSDDVIKS
                     SGYR"
BASE COUNT         1441 a         2936 c         3041 g         1616 t
ORIGIN      
        1 gacggcgaac cggtcatcac catcatgctg ccggatgagg actgggagac gatcgggtgc
       61 ggccgccaca gcgcgggcga ccggatgctc accgtcgacg acgtcgcctc gaccctgaac
      121 ttcacccccg gccggatccg ggagttcatc cgcgaagagc ggatcccggc ggtcaagtgc
      181 gggggatcgt ggcggatcaa gcggtcggaa ctcgagcgga tcatgaacga gggtttctga
      241 tcccggcctt acttatgagt tttctcacgg gccttgaact ggaaacaaac ctatttaatt
      301 tttagacgta gagttctcct tatgtgctcc ggggcctcac gagaactccc gaacgcctct
      361 ctcatcgacc ctgtcgccgg actcgacgat ggtgcggcag aacggacata ctgtgcggac
      421 gggtgcgcgc ccgggcccgg gcgggactgc gggcggcggt cgtgcatctt gctgtccctg
      481 ttcttctgcg ttgcccttct cgctctcctc ttctccacgc cggcagacct ctggacacct
      541 ccggcgatag aacccctctc gcccatcgtg ggattgcccg gtatattcgg tccggatgcg
      601 cgctcgttct tcaaacgctg gagagggcgg cgagccgggg aggaagaggc gcctccggat
      661 ccggccccgg tcgagcactc ggggagcgag gccggcgcac ccgtgaagct tgaagaggcc
      721 cctgcgaact ccctgcagct cgacgacccc gtcctcccgg acagcgcgga tgccgccggg
      781 aagaccccgg aggagttctt ctggtttgac gatcccctct tgcctgacgg ggccgatgcg
      841 ctgcccgtcg agcaggagcc ggacgattgc tccggcgagg cgggggaact cccggagcct
      901 cctccccgct ccgaacctct caccgatgcg gagctcatga ggctgctcgg gctcgacgat
      961 gaagacgatg aaccctcgtc cgggactccg ggttaccgcg ccttcccggc ggaggatgaa
     1021 ccgacctttg cgcccggcga agatcgcccc gtggagtcgc tcatcgacga cctcggcagc
     1081 cccgatgccg atatccgcag gcgtgccgtc gacgccatca gagagcacgg ggccgatgcc
     1141 gtggctcccc tggtagaggc cctcgctcgc gcggacgacg ggcggcggtg gtgcgtcgca
     1201 gaagccctcg ccgcgatcgg tgacgacgca atcccggcgc tgatcgcggc tctcggtgac
     1261 gatgcggtgc agaccggtgc tgccgccacg ctcgtgcgga taggggagcc cgccgtgccg
     1321 cccctcatcg ctgcactcgc cggcgatgac gacgaggtgc agttcggcgc tttctacgct
     1381 ctccgggaga tcggcgatac ggcgatcccc ttccttgtcg gagcccttga tgctcctgac
     1441 gggggcgccc ggcgatctgc cgcacggctc ctcagagaac tcgggtggga acctcccgat
     1501 gatccggggg cgatccggta cctcatcgcc agcgaggcat ggctcgacgt cgcggagtac
     1561 ggcggggccg cggtcggccc tctcatcgcc atcctgcgat cccctgacag ggaggcccgg
     1621 tggagtgccg cccgaaccct cggggagatc ggaaagaccg cggtcggccc cctggtcgat
     1681 ctcctggccg atgcggatga cgatatccga tggctcgcgg tcatggcgct cgcggagatc
     1741 ggttctcctg cggtcggttc gctcatccgg ctgctctccg atccctccct ctgcggcacg
     1801 gcgacggcgg ccctgctcag gataggggag ccggcggcgg aggagtgcgt ccacgccctg
     1861 gacgatgcgg atggggatgc gctcgaggcg ctccaggaag tcctcagtgc gctcggggag
     1921 gcggcggtcc cgcccctgat ccaggcgctg acagccagcc ggtcccggac ccgcggtcat
     1981 gccgccgaga tcctggatgg tatgggctgg gagccctgga atgatgccga gcgggcgtgg
     2041 tacctcatcg cccgggaaca gtggatcgag cttgccctga tgggtgaagc ggcggtcgtt
     2101 cccctgatcc ggacgctcaa cggcgatgac gaccacgtac gcagcgaggc tgcagcgacg
     2161 ctcggtgaga tcggtcatcc cgcggctgta ctgccgctcg tagatgtcct tgccaacgat
     2221 gccgtcgccc cggcagccgc ggatgcactt gttgccatag ggaggccggc cgtcgatccg
     2281 gttcttgccc tgctcgaggg ggggatcggc accgtccggg agcacgccgt cgagatcctg
     2341 ggccgtctcg gtgcgcacga ggcggtcccg accatcgtcg acctcgtcag gagcgggggc
     2401 gaccgcctcc accggaaggc cgtcgacgcc ctggtcggca tcggtgcgcc tgcggtgggc
     2461 gctctcatcc ccctcctcgg tgaggacggc gacggatatg caggggcgga ggctgcgctc
     2521 accggtatcg gcgatgccgc gcgggagcca ctcatcaagg cgctcggcga tgaggatgcc
     2581 cggacccgca tgggggccgc gatggtcctc aggagggttg gccgggcacc cgccggggtg
     2641 gaaggagagg ccgcgtacct gatcgcgctg cagcagtggc cggaggtcgt gggtctcggc
     2701 gcccccgccg tcgatctcct ggcggcgcgc cttggcgatc gggatgcagg ggtgcgggcg
     2761 ggggctgcgg agtcgcttgc ccgccttggg gccgttgcgg ctccccatct ggtcagcctg
     2821 ctcggcaacg aggctcgccg gggggctgcg ggcgagacgc tggtccggat cggggaggca
     2881 gcggtcccgc acctgatccg ggccctgggc gaggaggcgc tccgccggcc ggcagccggg
     2941 gtgctggtcc ggatagggaa tcctgccgcc ggccccctcg tccgggttct cggggaccct
     3001 gataccgggc aggttgcggc gaagatcctc ggggcgatgg gggagtcttc catcggcgca
     3061 ctggtcgggg ccctcgggga cgacgatatc caggtccggc agggagccgg gggcatcctg
     3121 attggtcttg gtgatgcggg catcggcgcg ctcatcggcg cgctggggca tccggacgac
     3181 gccgtccggc tggaagccgt cgatatgctc atccggatcg gtagatcggc cgtcgccgat
     3241 cttacggggg cgctcctcga tgagcgttac cgcgtccggc tgggcgctgc ggaggccctc
     3301 ggcaggattg gttgggcgcc ggagaccgag caggagacta tcggctacct gatcgcaaag
     3361 gagcagtggg tttccgtagc ggagtttgga gccgatgcgg tcatccccct gatccggacg
     3421 ctcaacgatt ccgacaccgc agtccagatg ggtgcggcgc gggcgctcgg catgatcggg
     3481 gcgcccgcgg tcacgaggct gcttggcgag ctccgtgacg ggcaggagga cgggcggaga
     3541 aaagcggtcg aagcgctcaa gatggtcggg gaggcggcgg tcaacccgct catcgatgcc
     3601 ctccaggacc gtgactggca cactcgcctg ggcgcggcgc gggcgctcgt tggcatcgga
     3661 aaccccgcta tcgagccgct gatccgggct cttcgcgacg gctccccggg catcaagatg
     3721 ggagcggccg caacgctcgg caagatagga aacccggccg ccatcgggcc gctcacggat
     3781 acgctcctgc acgaggactg gcgcgtgggg cgtgttgtcg tgcgggcgct cggcatgatg
     3841 ggcgagccgg cggtcgaggc gctcctcggc gtccttgaga aaggggacga tgcagcccgg
     3901 aaaggcgcgg tggcggctct tgtcctgatc ggcgaaccgg caaagcggct gctccccggc
     3961 gcacttgccg ccgggcattt ccgcatccgc gccggtgctg cggatgctct cgaccgcctg
     4021 ggctggtcgc cgggcccggg ggaggagatg gccctctacc tgattgcaaa ggaacgctgg
     4081 agcgatcttg tccggctggg cccgctcgcg gtcgagccgc tggtgcaggt gctcgacgac
     4141 cacgacgaca gcgtccggcg gcgggcggca aaggccctcg gcgaggtgcg cgacccccgt
     4201 gcggtccctg ccctcatggc cctcctccac gacgacttct acagcatccg gagggaggcc
     4261 gccgcagcgc ttgtcgcggt aggacctccg gccatggggc cggtcatatc cgcgctcggg
     4321 gatcctgacg gcgatgtcag gaaacgggcg gcggatgtgc ttgccggtat cggggatgca
     4381 cgggcggtcg agcccctcag gagcatcgag agggacgagg actggtatgt ccggaaggcg
     4441 gcggaggagg cggtggagaa gatccgggga cgcattggag agagcgtgta gggattccgc
     4501 tcgatcccgt agttactcgt gccgctggag gtactcccgg atcttccttg aatcgatcca
     4561 gccctggtcg agctgccgga tatacccgtt gatccgttcg atctcccgct ggatcttacc
     4621 ggtcgcctgc tcgtcctcca gcaggcatgc cgtgccgagg atcacctgga ggggctggcg
     4681 gatgtggtcg ccgaggatag cgaactgctc gatgttctgc ctgatccggt cgaatgcttc
     4741 ctgccgggcc tgttcgagtt cccgctcgcg ggtgatgtcg tgggatataa ccgcaactct
     4801 ccggctgtct tcctggtccc ccgggaccgg gaagaggacg ttttcgagga tccgcccctc
     4861 ccgctcatcg acatgccgga gaggttttcc cgtggcgagg gcctgtatgg taagctctcg
     4921 ccgccgcatc gccagttccg gcgtgaggat gtcgtaggcg catgccccga ggagctcctc
     4981 gacggttttt ttgaaccgcg tcgccatcac ctcgttcagg gcgagtatga taccgctatg
     5041 attgatgagc atggccgcat cgggtgtggc gtcaagaagc gcccggaact gctcctctgc
     5101 tcgccgccgc ccggtgacgt cgtggatgat ggagtgcagg agcacccgcc cgcagatgat
     5161 cacgcggccg ctgaagacgt ccacgtcgcg gtatcgcccg tccgccagcc ggtgcaggaa
     5221 cgagaactgc cgcttttcgc cggtcaccgc cttctgcatg tcccggaaga gatcttcccg
     5281 gtcaaggacg ttgaggtcgg cgatctgcat cgtggtgagg gtctcgagcg gatggccgta
     5341 gtaagcgctc gccgcggggt tcgcatcgac gatggcaccg gtctgtgggt cgacgatcag
     5401 catgaccgcg tggctcgtgg caaaaatgct ccggaacctg tcttcgtcct ccgcgatctg
     5461 ttccaggacg gcggtatcgg cgacgctcct cctggctgcc gtggttacgt ctcccgagcc
     5521 gggaacttcc tgttccgggg tccgcctttc tctgccgggc acgacgataa ggcgctctgt
     5581 cgccggggga ggcctctcct ccgcacgccc ggcacctccc tttccggtgt cgtggaagac
     5641 gaggagccgg agcgcgggcg tgacggcatc gaccgtgttt gccgtgacgg atatcctccg
     5701 gataccggtc ccgggaaccc ggacatcgag cgtgaccggc tgaggatacg atccgtcgca
     5761 gaggagggcg acgacctccc cgggagactc gccctccgga agcagcggca aaagatatct
     5821 ctgcacgaca tcgggagcat ccgctccgag gatctcttcc cgtgtcgcgc aaaggagggc
     5881 ctgcacctgt atgttagcct gaacaaccac ctggtcggcg tcgacgatga ggatcccgtc
     5941 gccctgcgca aggggagatg gcagggtctt acgcccaccc ggggatatcg ccggagtcat
     6001 ggatgagatg gttctctggt tcggttgaag gatctctttc gccggagaat aagtactttt
     6061 accatccgtc tcgtccgggc atgtacccgt gcctcccgcc ccatcactac agccgacagg
     6121 agtcctcgta ggacttcctg caccccggac agagcgtccg ggcctttctc aggtcgggga
     6181 atgtctcttc ggtgatctca tcgcaggccg ctccgcatat ctcgcaccag tactcttccg
     6241 tctcagcttt cccctcgcct tttctgtccc gtttcatgca acccctctcc gtgcccggtt
     6301 gtccggcccc gggcgggttc ctgctctcct ccatccggca tatgctttcc cccggatcac
     6361 catcgatcca ccccgacaag atcgagcccc atctcccaga accgttcctg cacgtccgca
     6421 tcgagagcaa gcggcgacgg ccgggtccac cgggtcgcct cgaagtacct gccgctcact
     6481 tctcccgcat ccggcgatgt ggcaaggtgc gcctcgaccg ccgccccctt ctcgggtggt
     6541 gcaccaccgt cccgcccgcc ggtgtaggcc cggaggagtt tggtatcgat cacccccggg
     6601 tggagggagt tcgccgtcac ccccgtcccg tcgaggacgg aggcgagccg ggcggtgcag
     6661 gcggcgactc cgagttttga cgttgcatag gcaccgtagg cgtcgtaatc ctcgaaggcc
     6721 ggaatattct cccagtcgac cgcccgaacg ctccggtggg cgatcgatgc gacgttgacg
     6781 acccgcgccg gtgcgctctt cgacaggagc gggaggaggc cgtgggtgag caggagccct
     6841 gcaaggtagt tcacggcaaa cgtcatctcg atcccgccgg gtgcaacgta ccgctccggc
     6901 atgaagaccc cggcattgtt gatgagcacg tcgagccggt cgcatgcccc ggcgacctcc
     6961 tctgcaaggt cccggacccg gtcctgcacc gagagatcgg cgatgaagag ggccggttgc
     7021 tccgggacgg cggctgcctc cacctcctca aggacggcgc gccccttcgc ccggtccctg
     7081 ccgtggatca ggatgcggtg gccctgttcg gccaggagat atgctgctgc cctcccgata
     7141 ccgtccgtcg agcccgtgat caggatggtc ttcttcgcca tacgcggcca gggtgcgcac
     7201 cgtaattatt tgaacgttgt gtgccggggc ggcaatccgg tgcaccctcc aggcaggggc
     7261 ggcagcggtg accggacacg aggtccgtac cctgcaccac ccctcccttt ccgtggcggg
     7321 tggaggaccg gcacggtctt tccggttcag gccgggtgcg cccctcccct cggggccggc
     7381 accgttcacc tctcctccgc ccggcttcgg gtctttctgc accgctgaga accccggtca
     7441 tcgatttctg gtgcgagatg aatcaaaaag gtgatatgtt gcaccacaga actgtacgta
     7501 tggtgcgata ctctgtcacg atggacgatg accttgtgaa gacgatcgac aggatgtgcg
     7561 actctctcgg gatgtcccgc tccgaatggc tcaataaact ctgtacggcc cacctgagtg
     7621 gtgcgccggg taacggtggc gggcagggcg tggccccgac attgatgccg cctctgcaag
     7681 cccccggtcc caacatgacc gattacgaga ccgcctgcgc cagtttcaag atcgacgtcc
     7741 ccgattattt caacttcgga tatgacgtga tcgaccggtg ggcagagacc gaccggaaca
     7801 aactggcaat gatctgggtg gaccagcagg gggccgagaa gaagtactcc ttccgcgacc
     7861 tccggaacct ctccaacggc gccgcgaaca ttctcctgaa gtatggcgtc aagaagggtg
     7921 accgggtcat gctgatactt ccgcggatac cggaatggtg gatctttgtt atcgcgctca
     7981 tcaagcttgg agcgatcttc tgcccatgcc cgacgatgct gacccctaaa gatatcagat
     8041 accgtatacg ggcggggaag ttcaggatga tcatcaccaa caccgagaac gccgagaaga
     8101 tcgatgaagt cgctgatgcc tgcccgctgc tcgaaaccct cttcctcgtg gatggggagc
     8161 gggacggttg ggcgagttac ctgcacgagc tcgattaccc tgcaccggta tcgcgccaca
     8221 ccgtcagcat gccggtcgca aagaggacga agtcgaccga cccgatgatg atctacttca
     8281 cctcgggcac caccggtgag ccgaagatgg tgctgcacaa ccagggctac ccgctcgggc
     8341 acatcataac cgcctcgctc tggcaggatc tcaccccaaa tgaccttcac ctgacgttct
     8401 cggataccgg gtgggcgaag tgtgcctggg gcaagatctt cggtcagtgg atcgccggcg
     8461 cctgcatctt tgtctatgat atccagggta agttcggcgc gaccgagctc cttccgctga
     8521 tagagaagta cgaggtgacc acgttctgtg cgccgccgac ggtctaccgg atgctgatcc
     8581 ttgccgatct cgataagttc gacttccgga acctccggca ctgctgcagc gccggcgaac
     8641 cgctcaaccc cgaggtgatc cgcgtctggc aggacggcac cgggcaaccc atctatgagg
     8701 ggtacggcca gacggagacc gtctgctgca tcgccacgtt cccctgcatg gagcataaac
     8761 ccggttcgat gggtagaccc gtcccgggct ggcatatcga gctccacgac gatgatggga
     8821 acccggtcgg tgccggggag gaggggaaga ttgctgtcag cctcaatccc cggccggtcg
     8881 gactcttcat ggagtacctt gacaaccccg aagcgaaccg cgagtcgttc cagaacgggt
     8941 tctactacac cggcgacaag gcacgtatgg acgaggatgg ctacctctgg ttcatcggac
     9001 gcagtgacga cgtcatcaag tcgtccggct accg
//
LOCUS       DAOSEE010000088         8859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_2920_length_8859_cov_220.095525, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000088 DAOSEE010000000
VERSION     DAOSEE010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8859
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2920_length_8859_cov_220.095525"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2224)
                     /locus_tag="PLI76_07565"
     CDS             complement(<1..2224)
                     /locus_tag="PLI76_07565"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HOI61927.1"
                     /translation="MAKGEVDPAETRPGEAVTPEEITEVPPFIITGIGASAGGLEALE
                     TFLKNTPPDIGAGFVVVTHMDPEHRSLLPEILQRSTTMPVVQIEHGMKVRQNSVYVIP
                     PNTNLTIQKGILNLEEPSRPRGMRLPIDHFFRGLAQDQDSKAVGILLSGMGHDGVLGL
                     RSLKERLGTVMVQDPITAEFPSMPRSVIVSGLADYIAPAGELPKLLANYIQSYRSLRA
                     ANPEEKRYLNTRTKILALIRSRTGEDYSIFKESLIRGRIERRMGLHRITDGEDYVRYV
                     QEHPREIDFLAQEIPTGPNWFFRDPGVWKILRERLLGELIRAKPGDSTLRVWVSGCAT
                     GEQAYSMAIALEETIQSSGSDIQYQIFATDIDRESILEARGGIYIANIEVDVSPEQLD
                     RFFTRQDNTYQIRQEIREKTIFAQHNILTHPPFLHLDILDVRSLLLYTSPDVVQKLIP
                     LFHYALNPGGILILGIDYSAGEPLEHFETIDATWKIYRRAAGPGGDDIQVELPSTLIP
                     SFGLVVGEPRLRKENEGSSIAALAREWLLSQYAPPAVIVNENGDILYFQGRTGKYLEP
                     HPGRATLNVHTMAREGLRYPLASTTRTALQEKRTVTRGPVFVQTNGSEEAIRLTVRPI
                     REIEGAEDLLMVVFEPVQDPAPEVGHEEGAAWKAATQPDLDKELAETRAQLQRTVEDM
                     QASQEELKSMNEELQSTNEELKSTNEELTSSKEELQSLNEELLTVNAEHQRKIEELSE
                     S"
     gene            complement(2433..2744)
                     /locus_tag="PLI76_07570"
     CDS             complement(2433..2744)
                     /locus_tag="PLI76_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOI61928.1"
                     /translation="MAAVTAEQVREAAGVLGIRDRASLNEIRRRYHDRIKEWHPDVSQ
                     NDPVKSHEMTLRLKEAYDLLVDYCMNRTFSFRTEDIAEGLGQNPTRYWMDRFGDDPIW
                     G"
     gene            2946..4400
                     /locus_tag="PLI76_07575"
     CDS             2946..4400
                     /locus_tag="PLI76_07575"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HOI61929.1"
                     /translation="MRPAPGEVIAMTGSSGAAIRLFDYGPIFLLRHCLRDLHLDRHLP
                     ELFPETEAAMVVALAFNRLIRPATMPYIDTWYADTALALDNPALSLSDREIGELLKKI
                     GTSDIPDILADLIIEENETRRALVYDITSLSGSSSLEHGYSQDSLLLPPITLSLVVDE
                     DRGIPVMYDVYPGSITDSTTLAGTLARLEACGVTDCTAILGSGFFSSDNLLDMLDEQI
                     SFVIAARFSMKELRALITREQRDIARAGYIREFHGRTIHVKPVVFALEGRALQGYLYY
                     DPGLEQEAREAFTRHLDDIRAALFAVRLKPGQIADHVFSGIAGEFGGFFDCKQVEDRI
                     EATIRQSAVTLRTNRMGRYMLFYQGNADWMTCLSRYRERDCIEEEFERLKGELEALPL
                     EAHGTAAFHGYLFIVFLSLMLRSRLARMLEEAGLAETYSVEGVMLELEKWKKIVLADG
                     RVTTTRMTRKQKTILKALGLVPLPREAKEKGRPR"
     gene            4731..5927
                     /locus_tag="PLI76_07580"
     CDS             4731..5927
                     /locus_tag="PLI76_07580"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOI61930.1"
                     /translation="MTKVFLCTNGCVEGQLRTKFVERYLGAHAQNIHMASAIGDADLV
                     LFYACGLTGEKEGHSLSVIDELKEKMRQDAELVVWGCLPKQNPGALVGVYDGPIIGPM
                     DTGSITSLIGTNGVSPESIDRSAAASELATMASPSIHGCNGTDLLTAAFLLYKRGVDL
                     FGSRRQRRSEVYHIPIAAGCTGHCTYCSERPVFGNTVHSRPIDDIVSEFRGGLERGYS
                     RFSLFATDLGSYGIDIGCRLPDLLRDLVAIGPAENCSILLNQIEPHRFEEVYGDMEPI
                     LASGKIEMVMAPVQSGSNRILKLMGRHYTAERWSALMLDIYRNYPAIRLSTQFMVGFP
                     TETEDDFAATLSLLDPPLRLDEAHIFRFSPRPTVAASRLPGQVPDDVKEARAARLLSR
                     FARRRI"
     gene            6211..7683
                     /locus_tag="PLI76_07585"
     CDS             6211..7683
                     /locus_tag="PLI76_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOI61931.1"
                     /translation="MIRKMACTGFLVILVASVSMAVLLAFIQAGGIHTAGIPEKNAIL
                     VAARDSPESAKALADYICDGVADQVEIQQAIDASRSRGMIVSLAEGTFTLDGDLVVPD
                     DTTIEGKGDTRTHLHWISGRLTAYGGENIVLRDFETCGTGAIFFFNCSHVKVHDVTAR
                     IDNSLGGGAFFVWVQNSVSEDIEFVNCKAIDCGRMGFMNDGEGARRLIRDIRYINCQA
                     INSGRHERFAPYGEWTTGFAIAENNDLADAVLTGCLAEGSFESGFHVEDAPTVTNLVF
                     RNCISRNNGQKPDAFYNPSEESHGLHFGSGYWVQGETTLYNCVSEGNGNAGFSAGPGV
                     RLYSCTDDGSAVGFRLVETHDVYIEDCTSRNAGAYGVYALEAERIVAEGLEILDPAGF
                     DGSGSFFGTTAHPVVDSRFDIVGRNSETIRTIYGEGCRDVTFTGVIRTDHREPVVIAR
                     GAGVDTRGLQVEAKEEIASPGQDGGCGLKNFLIRIITAFL"
     gene            7680..8822
                     /locus_tag="PLI76_07590"
     CDS             7680..8822
                     /locus_tag="PLI76_07590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOI61932.1"
                     /translation="MKIVQVTAYYPPHTGGIERYVHNLSRALAGRGHDVTVLTANVPR
                     GRPFETENGVAIERRPCLVRPFNDPFVPSFFRPGESLAGVDVLHAHNAYSCAALSAGV
                     VRRKFGVPTVLTHHGQHMFGHPLKDLFVTLYRGTIEHGLVHRVDRVVTLSPSDAAYVS
                     AFGVGRERISVIPNAICPEDFLPCTSEDTAGFEEAHRLEGKKRVLFVGQISTRKGVDV
                     LLRAVDQIVHEQGRDDLVFLFVGDGDYRERAQELARRHRVEEYVRFAGKVGLCDLVAA
                     YRSSDVYVLPSLSEGMPTVILEAMFFGLPVITTDLPCLRDNFGDHAVLVPPRDEHALA
                     RAVTGLLDDAARCRRLSERGKELVRSNYTWDNVVLAYERLYEQMTD"
BASE COUNT         1534 a         2720 c         2881 g         1724 t
ORIGIN      
        1 tgctctccga gagctcctcg atcttcctct ggtgttcggc gttgacggtg agcagttcct
       61 cgtttaagga ttggagttcc tccttcgaac tcgtcaactc ctcgttcgtg ctcttcagtt
      121 cctcgttcgt gctctggagc tcctcgttca tcgacttgag ttcttcctgg gaggcctgca
      181 tatcctcgac cgtgcgctgg agttgggccc tggtctcggc gagttcttta tcgagatcgg
      241 gctgcgtggc agccttccat gccgccccct cttcatgccc cacctccggg gcggggtcct
      301 gcaccggctc gaagacgacc atgaggagat cctctgcgcc ctcgatctcg cgtatcgggc
      361 ggacggtcag gcggatagcc tcttcgctcc cgttcgtctg cacgaagacc ggcccccgcg
      421 tgacggtgcg cttctcctgg agagcggtgc gggtggtgga ggcaagcgga tagcggagac
      481 cctcccgcgc catcgtatgg acgttcaggg tggctctgcc cggatggggt tcgagatact
      541 tcccggtcct cccctggaag tacaggatgt cgccgttctc gttcacgatc accgccggcg
      601 gggcatactg cgagagtagc cactcccggg cgagtgcggc gatggaggac ccttcgttct
      661 ccttccggag cctgggttcg cccaccacga gcccgaacga ggggatgagg gtggagggca
      721 gttctacctg gatatcatcc cctccgggcc ccgccgcccg ccggtagatc ttccatgtcg
      781 catcgatggt ctcgaagtgc tccaggggtt cgccggcgga gtagtcgatg ccgaggatga
      841 ggatcccgcc gggattcaag gcataatgaa agagaggtat cagtttctgc accacatccg
      901 ggctcgtgta gaggagaagg ctccggacgt ccaggatgtc caggtggagg aacggcggat
      961 gggtcagtat gttatgctgg gcaaatatgg tcttttcgcg gatctcctgg cggatctggt
     1021 aggtgttgtc ctgccgggta aagaagcgat ccagttgctc gggggataca tccacctcga
     1081 tgtttgcgat gtagatcccc ccccgcgctt cgaggatgct ctcccggtcg atgtcggtcg
     1141 caaagatctg gtactggata tcgctgcccg aggactggat cgtctcttcg agcgcaatgg
     1201 ccatgctgta ggcctgctcc ccggtggcgc accccgagac ccagacgcgg agcgtgctgt
     1261 caccgggttt tgcccggatc aactcgccga gcagcctctc gcggaggatc ttccataccc
     1321 cggggtcgcg gaagaaccag ttcggaccgg tggggatctc ctgcgcgagg aaatcgatct
     1381 ctcgtgggtg ttcctggacg tagcgcacgt agtcttcgcc gtcggtgatc cggtgcagac
     1441 ccatccgccg ctcgatcctg ccgcggatca ggctctcctt gaatatggag tagtcttcgc
     1501 ccgtccgcga gcggatcagg gcaaggatct tcgtgcgcgt attcaggtac cgcttctcct
     1561 ccgggttcgc cgcgcgaagg ctccggtagg actggatata attggcaagc agtttcggga
     1621 gctcgcccgc cggcgctatg tagtcggcaa gcccggagac gatgacgctc cggggcatgc
     1681 tcgggaactc tgcggtgatc gggtcctgga ccatcaccgt cccgaggcgc tcttttaacg
     1741 agcggaggcc gagcaccccg tcgtgcccca tgccggagag gaggatgccg accgcttttg
     1801 agtcctggtc ctgagcgaga ccgcgaaaga agtggtcgat gggcagccgc atccccctcg
     1861 gtctggacgg ttcttcgagg ttgaggatgc ctttctggat ggtgaggttg gtgtttgggg
     1921 ggatcacgta gaccgagttt tgccggacct tcatgccgtg ctcgatctgt accaccggca
     1981 tcgtggtgct tcgctggagg atctcgggca gaaggctcct gtgttcgggg tccatgtggg
     2041 tgaccaccac gaaccctgcg ccgatatcgg ggggcgtgtt cttgaggaag gtctcaaggg
     2101 cctcaagccc cccggcagat gcgccgatgc cggtgatgat gaagggcggc acttctgtga
     2161 tctcttccgg tgtaacggct tctcctggtc ttgtttctgc tggatctacc tctcctttcg
     2221 ccataccacg gtcgccctca ttcctccggt cgggatgtga gtacatctat gcacccggca
     2281 ttaaacctgt tgcaccgggg tccgcgcgac cgccgtgatg ccggtgcccg gagaccccgc
     2341 ctgcgatgcc cggttggctc cggtggaggt gcacccggtg acggcggagg cctgcgccac
     2401 gtttgccggc tccggggatg gcgggcggag tactaccccc agatcgggtc gtcgccgaac
     2461 cggtccatcc agtacctggt ggggttctgc cccaggccct cagcgatatc ttcggtccgg
     2521 aaagagaacg tccggttcat gcagtaatcg accaggaggt cgtaggcctc cttcaggcgc
     2581 agggtcatct cgtgggattt taccgggtcg ttctgcgaca cgtcggggtg ccactccttg
     2641 atccgatcgt gataacgtct ccggatctcg ttgaggctgg cacggtcccg gatgccgagc
     2701 accccggccg cctccctcac ctgctcggcg gtaactgcag ccatcaggga tctgtggacg
     2761 ttcccgcagg aaaaatctgc cggagtatcc agagcagccc cctgccggcc ggggtgcccg
     2821 gcaccgcgag accggtacgg cggatcagcg ggattacata gaggtgcgaa gagatcctgt
     2881 accatgatcc ggtcttcaga aagacccggc accctgaccg gaacgccggc ggtgaaccaa
     2941 tccgtatgcg gccagcccct ggtgaggtca ttgccatgac cgggagctcg ggggcggcta
     3001 tccggttgtt cgactacggc ccgatcttcc tcctccggca ctgcctgcgt gacctgcacc
     3061 tcgatcggca cctccccgaa ctcttcccgg agaccgaggc cgccatggtc gtcgcgctcg
     3121 ccttcaaccg ccttatccgg ccggcaacga tgccctatat cgacacctgg tatgcggata
     3181 ccgccctcgc cctggacaac ccggccctct ccctctccga tcgagagatc ggtgaactcc
     3241 tcaagaagat cggcacgagc gatataccgg atatcctggc ggatctgatt atcgaggaga
     3301 acgagacgcg gcgagccctg gtgtacgaca tcaccagcct ctcgggctcc tcctcgctcg
     3361 agcacgggta tagccaggat agcctgctcc tgcccccgat caccctctcc ctggtcgtgg
     3421 atgaggaccg cggcatcccg gtgatgtatg atgtctatcc gggcagcatc accgatagca
     3481 ccaccctcgc cggcaccctt gcaaggctcg aggcctgcgg ggtcacggac tgcaccgcca
     3541 tcctggggag cgggttcttc agttcggaca acctgctgga tatgctcgat gagcagatat
     3601 cgttcgttat tgccgccagg ttcagcatga aagagctcag ggcgctgatc accagggagc
     3661 agcgggatat cgcccgggcc gggtatatcc gggagttcca cggccggaca atccatgtca
     3721 aaccggtggt ttttgccctc gaaggccgtg cgttgcaggg ctacctgtat tacgaccccg
     3781 ggcttgagca ggaagcgaga gaggcgttca cccgccacct ggatgatatc cgggcggcgc
     3841 tctttgccgt gaggctgaag ccgggccaga tcgcggatca cgtcttctcc gggatagccg
     3901 gggagtttgg gggttttttt gactgcaagc aggtggagga ccggatcgag gcgaccatcc
     3961 gccagagcgc ggtaaccctg cggacgaacc ggatgggaag atacatgctc ttttaccagg
     4021 ggaacgcgga ctggatgacg tgcctctccc ggtacaggga gcgggactgt atcgaagagg
     4081 agttcgagag gctgaagggt gaactggagg ctctccctct ggaggcgcac ggcaccgccg
     4141 cgttccatgg ctacctcttc atcgtgtttc tcagcctgat gctgcggtcg cggctggcga
     4201 ggatgctgga ggaggcgggg ctggcagaga cgtattcggt ggaaggggtg atgcttgagc
     4261 tggagaagtg gaagaagatc gtcctggcgg acggccgggt tacgacgacc cggatgaccc
     4321 ggaaacagaa gacgatcctg aaggcgctcg gtctcgtgcc cctcccccgc gaggccaaag
     4381 agaagggccg gccacggtaa accccgggtg ctcaccggct ccctggattg cacgttcgcc
     4441 tcagccgggg gccggatata ccggattgcg agcggggaga gttgccgcag atggcgttcc
     4501 cggtatcccc tgcctggagg ttgacgatgg ccccggcaaa tcccgccggg gaccggcccg
     4561 ggtacgcggc gctataggga gcagccccct ccgggctgca ccaggtgatg cctcatgtgc
     4621 cggcaggata ccccgctctc ctgattctcc cggaaggcgg cgaggtcgat ccggctcccg
     4681 gcaaaagttt tatctatggc aggtgctata gggtgctttc gcggtaacct atgacgaaag
     4741 tattcctctg tacgaacggg tgcgtcgagg ggcagctcag gacaaaattc gtcgagaggt
     4801 acctgggtgc tcacgctcag aatatccata tggcgtcggc gataggcgat gccgatctcg
     4861 ttctcttcta tgcgtgtggt ctgaccggcg agaaagaggg ccattcgctg agcgtcatcg
     4921 atgaactcaa ggagaagatg cggcaggatg ccgaactcgt cgtctggggt tgcctcccga
     4981 aacagaaccc cggggcgctt gtaggagtct acgacggccc gatcatcggt ccgatggata
     5041 ccggctccat tacctctctt ataggcacga acggcgtctc cccggagagc atcgaccgct
     5101 cggcggccgc aagcgagctt gcgaccatgg cctcgcccag tatccacggc tgcaacggca
     5161 ccgacctgct caccgccgcg ttcctcctct ataaacgggg ggtggacctc ttcggctcgc
     5221 gccggcagag acgctccgag gtgtatcaca tacctatcgc ggccgggtgc accgggcatt
     5281 gcacctactg cagcgaacgg ccggtctttg ggaataccgt ccacagccgt cccatcgatg
     5341 acatcgtatc ggagttccgg ggcgggctcg agcgtggata tagccgtttc tccctgttcg
     5401 ctacagatct cgggagttac ggcatcgata tcggttgccg cctccccgac ctcttgagag
     5461 acctggtcgc catcggtccg gcggagaact gttcgatcct cctgaaccag atcgaaccgc
     5521 accgctttga agaggtttac ggcgacatgg agcccatcct cgcatccggt aagatcgaga
     5581 tggtgatggc gcccgtccag tcggggagca accggatcct gaaactcatg ggccggcact
     5641 acaccgccga gcggtggagc gcgctgatgc tggacatcta ccggaactac cccgctatcc
     5701 ggttgagcac ccagttcatg gtggggttcc cgacggagac ggaggacgac ttcgctgcaa
     5761 cgctctctct cctcgatccg ccgctcagac tcgacgaagc gcatatattc aggttctccc
     5821 cgcggccgac ggttgccgcc tcccgccttc cgggccaggt tcccgatgac gtcaaggagg
     5881 cccgggctgc acgcctgctg agcaggtttg caaggaggcg tatatagccg gtgagagcgg
     5941 accagagccc gctgccttcc gggagcgtcc gcccgcccac ccgcaccggg cggcggacca
     6001 tacctctccg ataggagagg ccggaaagat cccggcattt gttcatcgcc gtagcgacag
     6061 gagcgggtcg tgtacgggcc ggcatgtgca gccacctccg gcccgccggt tgctcctggt
     6121 cgaggatgcg gccgtgcccc gtgaccgcaa cgttattata gcccggtgct gtattgcaga
     6181 atcgtctttt gggggatgga aagggttcgc atgattcgga agatggcgtg tacggggttc
     6241 ctcgtgatcc tcgttgcctc tgtgagcatg gcggttctcc tcgcctttat ccaggcaggc
     6301 ggcatacata ccgccggtat cccggagaag aacgccatac tggtcgctgc ccgcgatagc
     6361 ccggagtcgg caaaggcgct cgcggattac atctgcgacg gcgttgccga ccaggtggag
     6421 atccagcagg ccatcgatgc gtcccggtcg agagggatga tcgtctctct cgcagaaggg
     6481 acgttcaccc ttgatggaga cctggtggtg cccgatgata ccactatcga ggggaagggc
     6541 gataccagaa cccacctcca ctggatatcc ggccggctta ctgcgtacgg gggggagaat
     6601 atcgtcctta gagactttga gacctgcggg accggagcga tattcttctt caattgcagc
     6661 cacgtgaaag ttcacgacgt caccgcacgc atcgataact cccttggcgg gggggcgttc
     6721 ttcgtctggg tgcagaacag cgtatccgag gatatcgagt tcgtcaactg taaggccatt
     6781 gactgcggga ggatggggtt catgaacgac ggcgaggggg cccggagact catccgggat
     6841 atccgctaca tcaactgcca ggccatcaac tcgggaaggc acgaaaggtt cgccccctac
     6901 ggggagtgga cgaccggctt tgccatagcg gagaacaacg accttgccga tgcggtgctc
     6961 accggctgtc tggccgaggg ttcgtttgag tccgggtttc acgtcgaaga cgcccccacg
     7021 gtaacgaacc tggtattccg gaactgtatc agcaggaata acggccagaa accggatgcc
     7081 ttctacaacc cctcggagga gtcgcacggg ctccacttcg gttcgggcta ctgggtgcag
     7141 ggagagacga cgctctacaa ctgcgtatcc gaaggcaacg gcaatgcggg tttctccgcg
     7201 ggtcccgggg tccgcctcta cagctgcacc gacgacggtt cggcggtggg gttccgcctg
     7261 gtggagacgc atgatgtcta catcgaggac tgcaccagcc ggaacgccgg ggcgtatggg
     7321 gtctacgcgc tcgaggcgga gaggatcgtg gcggagggcc tggagatcct cgatccggcc
     7381 ggtttcgacg ggagcggttc gttcttcggc acgacggcgc acccggtcgt cgacagccgg
     7441 tttgacatcg tcggccggaa cagcgagacc atccgcacga tctacggcga aggctgccgc
     7501 gacgtcacgt tcacgggcgt catccggacc gatcacagag agccggtggt catcgcgaga
     7561 ggggcaggtg tcgatacccg cggtctgcag gtcgaggcga aagaggagat cgcgagcccc
     7621 ggccaggatg gcggctgcgg gctgaagaac ttcctgatca ggatcatcac ggcgtttcta
     7681 tgaagatagt ccaggttact gcctactacc cgccgcacac cgggggcatc gagcgctacg
     7741 tccataacct gagcagggcg cttgccggcc ggggccatga cgtcaccgtc ctgacggcga
     7801 acgtgccgag gggccgcccg ttcgagaccg agaacggggt ggcgatcgaa cgccgcccct
     7861 gtctggtgcg ccccttcaac gacccgtttg tgccctcgtt cttcaggccc ggcgagagcc
     7921 ttgccggagt cgacgtcctg cacgctcata atgcctacag ttgcgctgcg ttgagcgcgg
     7981 gcgtcgtccg gcggaagttc ggggtgccga cggtcctgac gcaccacggg cagcatatgt
     8041 tcgggcatcc cctcaaggac ctcttcgtga cgctgtaccg ggggaccatc gagcacgggc
     8101 ttgtccaccg ggtggaccgg gtcgtcaccc tctctccctc ggatgcggcg tacgtctcgg
     8161 cgttcggtgt cggccgggag cggatctccg tcataccaaa cgcgatctgc cccgaagact
     8221 tcctgccctg caccagcgag gatacggccg gatttgagga ggcgcacagg ctcgaaggga
     8281 agaagcgggt gctctttgtc gggcagatat cgacgaggaa gggcgtcgac gtgctcctgc
     8341 gggcggtcga ccagatcgtg cacgagcagg gccgggacga cctggtcttc ctcttcgtcg
     8401 gcgacgggga ttaccgggaa agagcgcagg agctcgcccg caggcaccgt gtcgaggagt
     8461 atgtccggtt cgccgggaag gtggggctct gcgacctcgt tgccgcatac cggtcgtcgg
     8521 acgtgtatgt cctcccctcg ctctcggagg ggatgcccac ggtgatcctg gaggccatgt
     8581 tcttcggcct gccggtcatc acgaccgacc tgccctgtct ccgggataac ttcggcgacc
     8641 atgccgttct ggtgcccccc cgcgacgagc acgctctggc ccgtgccgtc acggggctcc
     8701 ttgacgacgc cgcacgctgc cgccggctct cggagcgggg gaaggaactc gtccggtcga
     8761 actacacctg ggataacgtc gtgctggcat acgagcggct ctacgagcag atgaccgact
     8821 gagcggtcag ccggccccct ggtgcggcag aggaccggc
//
LOCUS       DAOSEE010000089         8832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_2931_length_8832_cov_255.694656, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000089 DAOSEE010000000
VERSION     DAOSEE010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8832
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2931_length_8832_cov_255.694656"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="PLI76_07595"
     CDS             complement(<1..815)
                     /locus_tag="PLI76_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3821 domain-containing protein"
                     /protein_id="HOI61933.1"
                     /translation="MAPEWIRGRGPVVLLALCLLALCAGDAGARGPTISDIQPYDTIF
                     VDEEGLNLSQLRNATTNNPITALQRYQDDNPGKSLVKSIPVADDTDFEVQEFLVGREY
                     GTYYAFNPADGTTASVFIRKPEIFLDIVLANPYHNEPLTGLTVSPNTRIAFRVAAPDV
                     GASYHVGNVYPATIDIVLTHPGGAETTLVGGINLAGLNITSTRFYTDDPGKPGAVRIG
                     DLGKPGDYTARAVWRMPAGFDTRASDSELITFTVGNRIGIDTTPTPTPTATPTA"
     gene            1010..2518
                     /locus_tag="PLI76_07600"
     CDS             1010..2518
                     /locus_tag="PLI76_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HOI61934.1"
                     /translation="MKKVAFIVLSILIFFAILAIPVDPALLAPEAKSVAAVTALMVLF
                     WVSGVIPLEATALLPLVLFPALGVLTPVQVAKSYGDEVIFLFLGGFIIAMSMQRWGLH
                     RRIALQIIRIVGTSPRRLVLGFMLATAFLSMWISNTATTMMMIPIAVAIILTVMPGIT
                     RSPENPDGEEQALARCLIVSIPYAASIGGIATIIGTPPNGIFISQLAAIFPDAPAIDF
                     FTWMKFGVPFAVAFLLLAWVWLTRGPYRHMVATLPSARGIIREELTKLGPISAGERWT
                     LIVFTLTALAWIFEQTKEIGGIVIPGLDIIFPGIKDSTIAIFGALLLFVLPVDTKEGI
                     FTMDWKWAVKIPWGILLLFGGGIALSSGFLESGLAAAIVESLTLIHGLPVILLLFVVA
                     MGIVLAGEIASNTAIAAILMPIMAVTAVSMGVNPIILMMTAAVCASIGFMLPVATPPN
                     AIAFGSGYINAKDLLRSGWVLDIIGVVLWMICLYTIVLWALGLPLDLPAWAL"
     gene            2640..4298
                     /locus_tag="PLI76_07605"
     CDS             2640..4298
                     /locus_tag="PLI76_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOI61935.1"
                     /translation="MLEDQRWQPVPGTIGAEIYPFFRKPDVTCSNAYVIRTSGDILII
                     DTGADAGQMDRLMLLIEEALRKAPRPVTLFITHCHIDHCYQAIRDRRFRSIPGFTVAA
                     EEAGARVLESGDTIRTAAELMGWEIEPLPVNLPLLSARDCETLEMGGGITLRRQAIPL
                     CSGDPLVVYHTPGHSPDSVCIRFGGLLFIGDLLFSTSPGVAGVAGWDQPALLASVGRI
                     RWILAHEPVNLCCPGHGRCIPVQAMPALLDRLEAEAERLSGIGAFDRERLDDSLEHTA
                     DLLQEANRVFTVIAGRLDALAYRLEDLGEADEARRYLELLKSDRIDEFLEEFAQFADE
                     FRTGKKIEVQLVLKAVQVIQRIESHFAGDRLNHIVDASLLRRTDRLLTDFMNTIMGYQ
                     DTVHLVATDLPSVLRDTVAGLSTLPFSDEAFIEAADDPATYRAELVVRLAYLPLFEGV
                     ALSLDTASPSLPPVLVDPERFSDEVVGVLGDLAAAGAVAIRLSLRQEGDAVGLLIQGS
                     DVFSARRLRFYRRKFGLAGASLAISRDGTGLFLVLQAVNRMRQA"
     gene            complement(4193..5512)
                     /gene="codB"
                     /locus_tag="PLI76_07610"
     CDS             complement(4193..5512)
                     /gene="codB"
                     /locus_tag="PLI76_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HOI61936.1"
                     /translation="MSGDERGRSGMEDYPLSAVPDEGRQEFWPITLVLLGFTFFSATM
                     WGGASIGVAFRFWPDLVWIILWGNAILAVYVAGLGYIGFRSGFSTVLSARFAFGDIGS
                     WWSDTLLGLTQLGWYAWGTATIAIALVRLLNLDAGCAAPLMIVFGLGFCMTAYVGCRG
                     LAALSWIAVPAMLLLIAVSIAVALRDAGSAAGPLAAGTLTVAEALTIVVGTFVSGGTQ
                     VANWTRFAGSGRVAVGSVLIAFFVGNGLMIAVGAVSAAIYGVSDIVEVLEIQGLLFAG
                     ILLLFLNIWTTQDNTIYNFSVAGCHFWRTERRRLITFGGAAAGTLLALAGMYDWLIPY
                     MVMMGVLIPPLGGVIMADFFVGHRGRYPPLDGAAVPRFNWRGLAAYAAGSLAALVVPG
                     IPPVNGIAAAFLIYACLIRFTAWRTRKRPVPSRDIASDAPARPNFRR"
     gene            5650..5847
                     /locus_tag="PLI76_07615"
     CDS             5650..5847
                     /locus_tag="PLI76_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61937.1"
                     /translation="MPRVRINEGDLVSGAGVEGRRKKKIDESPEPPREEPAGKEPAPV
                     KKGGRPSFGDADGADIFFTRK"
     gene            complement(5849..6214)
                     /locus_tag="PLI76_07620"
     CDS             complement(5849..6214)
                     /locus_tag="PLI76_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61938.1"
                     /translation="MLERQEKTALAVLICVVGIVLAVHLLFDAVGRPLVAAPYSEEVS
                     DGAFVLLEGRIEEIRKTSTGGHRILTVNGTAVFLPQDVAARLELDKNASVALYGVVQT
                     YRGEREVVVASAADIRVLK"
     gene            6285..6518
                     /locus_tag="PLI76_07625"
     CDS             6285..6518
                     /locus_tag="PLI76_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOI61939.1"
                     /translation="MDDVNLPPSSRKILLLLEDGGALTHKELVRLSSLAPRTVRYALK
                     RLKDNDMIVEKFNFRDARQILYEYKNSPMVSAQ"
     gene            6515..6832
                     /locus_tag="PLI76_07630"
     CDS             6515..6832
                     /locus_tag="PLI76_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOI61940.1"
                     /translation="MTEPPSCDIMRCDTLARRLLPRMRAEMVYRLVNERGISQSEASK
                     RLGISRAAISQYMSRKRGFTRQDFPGDLDVVIERWVSTIVSGEGTITICDVCRSAGLS
                     GRW"
     gene            complement(6836..8509)
                     /locus_tag="PLI76_07635"
     CDS             complement(6836..8509)
                     /locus_tag="PLI76_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOI61941.1"
                     /translation="MRRIAAIILLLCLLAGTAGGILIVEFCPDPYRPGDPDEYLVLEG
                     EGSLDRFVVSDGKGGFRFPPGTWINGRLVIARNSLAFEQTHGYLPDFEIEKRTAAVPD
                     VTSSGNLLMANQADALYLYHGATIVQQVAWPADVRPREGQVHYLEDGVWDPRPFFIGQ
                     SRFSSATFDDVTVTAFVAPDCAYDVFSAAVASAEREILVNVYEFTDPEMAADLIRARE
                     RGAAVTVLLEGGPVGGIPPEERAIAGILNRSGIVVLSMTTTDAAHAKYRYDHAKYVVI
                     DARTVLIGSENFKPGGYPAPGLQGNRGWGACLDDPKLAAYFREVLLFDLSGGDIVPLE
                     GTTADVRMPYAPAYTVEFTPCRAEGARVTPVLSPDTSALILRLIEGAGESIAIEQAYI
                     TNETTYELNPYLAAAIDASRRGVAVRVLLDACWFNIEDDADNDEMVDLINRIAAAEGL
                     PLEARIVDLTANNLAKVHNKGVIVDGRAVLVSSINWNANSPAFNREAGVIIEHPDITA
                     YFARVFADDWDAAAADGTVAEGPDRLKVVAAACILAALMGLYIYRRRRT"
     gene            8549..>8832
                     /locus_tag="PLI76_07640"
     CDS             8549..>8832
                     /locus_tag="PLI76_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridylate kinase"
                     /protein_id="HOI61942.1"
                     /translation="MTTPAPPLVVKVGGSLFDRVAPLLGILREVGRPVLIVPGGGKFA
                     DLVRRLAVSDTSAHWMAIAGMEQFGWYIASHGIPAVASPVLPAEATVLLP"
BASE COUNT         1514 a         2900 c         2794 g         1624 t
ORIGIN      
        1 gcggtcggcg tcgcggtcgg cgtcggcgtg ggggtcgtat cgataccgat ccgattcccg
       61 accgtgaacg ttatgagttc ggagtcggaa gcccgagtat cgaaaccggc cggcatcctc
      121 cagacggccc gggcggtgta gtcccccggc ttaccgaggt cgccgatccg gaccgcaccg
      181 ggtttacccg gatcgtcggt gtagaaccgg gtcgaggtga tgttcaatcc ggcaaggttg
      241 atcccgccga cgagggtggt ctccgcgccg cccgggtggg tgagcacgat atcgatcgtc
      301 gccgggtaga cgttgcccac atggtaggat gccccgacgt cgggtgcggc tacccggaac
      361 gctatccggg tgttcgggga taccgtgagg ccggtcagcg gttcgttgtg gtaggggttt
      421 gcgagtacga tgtcgaggaa gatctccggt ttgcggatga agacggatgc cgtcgtgccg
      481 tcggcggggt tgaaggcgta gtaggtcccg tactcacggc ccacgaggaa ctcctgcacc
      541 tcaaagtcgg tgtcgtcggc gaccgggatg ctctttacga gcgattttcc ggggttgtcg
      601 tcctggtacc tctggagcgc cgtgatgggg ttgttggtgg tcgcgttcct gagctgcgag
      661 aggttgagcc cctcctcatc tacgaagatc gtgtcgtagg gctggatatc gctgatcgtc
      721 gggccgcggg cccctgcatc tccggcacag agggcaagga ggcagagggc gaggaggacg
      781 acaggacctc gtccgcggat ccattctggt gccataatat tccttgtgtt gagtttgcaa
      841 atataggttg gtgcgggtca ggcccgcccg cccccggggt atacgggtgc ggtgagcgcg
      901 gcccggcggt atctgccggg atgaggcgat ccgttctcgc tccctgtcgc tccggcccgt
      961 gcgatctcaa ttccgcatgc ttaaataatt cttcctcaca ggttttagga tgaaaaaggt
     1021 cgccttcatc gtcctatcaa tcctcatatt ctttgcaatt ctggcgatcc ctgtcgatcc
     1081 tgctcttctt gcacctgagg caaagtccgt tgcggcggtg accgccctca tggtcctctt
     1141 ctgggtttca ggcgtcattc ccctggaggc gaccgccctc ctcccgctgg ttctcttccc
     1201 ggcgctcggc gtcctcaccc cggtgcaggt ggcgaagtcc tatggcgacg aggtgatctt
     1261 cctcttcctc ggcgggttca tcatcgccat gtcgatgcag cggtggggcc tgcaccggcg
     1321 gatcgccctg cagatcatcc ggattgtggg gacgagtccg aggcgcctgg tgctcgggtt
     1381 catgctcgcc accgccttcc tctcgatgtg gatatcgaat accgccacca cgatgatgat
     1441 gatcccgatt gcggttgcga tcatcctgac ggtcatgccc gggatcaccc ggtcccccga
     1501 gaaccctgac ggcgaagaac aggcgctcgc ccgctgcctg atcgtatcca tcccctacgc
     1561 cgcgagcatc ggaggcatcg ccacgatcat cggcacgccc ccgaacggga ttttcatatc
     1621 ccagcttgcg gcgatctttc ccgatgcgcc cgccatcgac ttcttcacct ggatgaagtt
     1681 cggcgtaccg ttcgctgtgg ccttcctcct ccttgcctgg gtctggctca cccggggccc
     1741 ctaccgccac atggtggcga ccctcccgag cgcccggggg atcatccggg aggagctcac
     1801 aaaactcggg cccatatcgg cgggcgagcg ctggacgctg atcgtcttca cgttgacggc
     1861 tcttgcctgg atattcgagc agaccaagga gatcggcggt atcgtcatcc ccggtctcga
     1921 tatcatcttc cccggcatca aagactcgac catcgcgata ttcggtgcgc tccttctctt
     1981 cgtcctcccg gtcgacacga aggagggcat cttcacgatg gactggaagt gggcggtgaa
     2041 gatcccctgg ggcatcctcc tcctctttgg cggcggtatc gccctctcaa gcggattcct
     2101 cgagagcggg cttgccgcgg ctatcgtcga gtctctcacc ctcatccacg gcctcccggt
     2161 aatccttctc ctcttcgtcg tggcgatggg gatcgtgctg gccggggaga tcgcctcgaa
     2221 caccgcgata gccgccatcc ttatgccgat catggcggtg acggccgtca gcatgggcgt
     2281 caacccgatc atcctgatga tgaccgcggc ggtctgcgcc tcgatcgggt tcatgctccc
     2341 ggtcgccaca cccccgaacg ccatcgcctt cgggagcggt tacatcaacg caaaagacct
     2401 tctccggtca ggctgggtgc tcgacatcat cggcgtcgtg ctctggatga tctgcctcta
     2461 cacgatcgtc ctctgggcgc tcgggcttcc cctcgacctc cccgcctggg cgctctgatc
     2521 cccggcacct tttttcatgg cggttctccc ggccaccttt tccccgggaa gaacccgccg
     2581 atcttttctc ctctgcggcg caacactctc cacgactctt ccctatcgag gcgcaaagaa
     2641 tgcttgaaga tcagcgatgg cagccggtgc ccggaaccat cggtgcagag atctatccct
     2701 tcttccggaa accggacgtc acctgctcga acgcctacgt catccggaca tccggcgata
     2761 tcctgatcat cgatacgggg gccgacgccg gccagatgga tcggctcatg ctgctcatcg
     2821 aggaggccct ccgaaaagcc ccgcggccgg tcaccctctt catcacccat tgccatatcg
     2881 accactgcta ccaggccatc cgggaccgac ggttccgttc catcccgggc ttcaccgtcg
     2941 ctgcagagga ggcgggagcc cgggtgcttg agtcgggcga tactatccgg acggctgcgg
     3001 aactcatggg gtgggagatc gagccgctcc cggtcaatct cccgctgctt tccgcccgcg
     3061 actgcgagac cctcgagatg ggcggcggga tcacccttcg ccggcaggcg atccccctct
     3121 gctccgggga tccccttgtg gtttatcaca cccccgggca ctcgcccgac agcgtatgca
     3181 tccggttcgg cggtctcctc ttcatcggcg acctcctctt ctccacaagc cccggggtcg
     3241 ccggggttgc cgggtgggat cagccggcgc tgctcgcatc cgtgggaagg atccggtgga
     3301 tcctggctca cgagccggtg aacctctgct gtcccggtca cggcagatgt atcccggttc
     3361 aggcgatgcc ggcgctcctc gaccggctcg aagcggaagc ggagagactc tccggtatcg
     3421 gcgccttcga ccgggagcgt ctcgacgact ccctggagca caccgccgat ctgctccagg
     3481 aggccaaccg ggtctttacg gtgatcgcgg ggcgcctcga cgccctcgct taccgcctcg
     3541 aagacctcgg cgaggccgac gaggcccgtc gctacctgga actgctcaaa tccgatcgga
     3601 tcgacgagtt tctggaggag ttcgcgcagt tcgccgacga gttccggacc ggaaagaaga
     3661 tcgaggtcca gctcgtcctc aaggccgtcc aggtcatcca gcggatcgaa tctcacttcg
     3721 cgggcgaccg gctcaaccac atcgtcgatg catcgctcct ccgcaggacc gaccgcctcc
     3781 tcaccgactt catgaacacg atcatgggct accaggacac ggttcacctt gtcgccacgg
     3841 accttccgtc ggtgctccgc gacacggtcg ccgggttatc aaccctgccc ttctcggacg
     3901 aggcgttcat cgaggcggcc gacgaccccg cgacataccg cgcggagctg gtcgtgcggc
     3961 tcgcctacct cccgctcttt gagggcgtcg ccctctctct tgataccgcc agcccctccc
     4021 tccctcctgt tctggtcgac ccggagcggt tctccgacga ggtcgtcggc gtgctcggcg
     4081 atctggcggc tgccggcgcc gtggcgatcc gcctctccct ccggcaggag ggcgatgccg
     4141 tcgggctcct gatccagggt tccgacgtct tctccgcgag gaggctccgg ttctaccggc
     4201 ggaagttcgg ccttgccggg gcgtcgctcg cgatatcgcg ggacggcacc ggcctcttcc
     4261 tcgtcctcca ggcggtgaac cggatgaggc aggcgtagat gaggaacgcc gcggcgatcc
     4321 cgttcaccgg cgggatgccg gggacgacga gcgcggcaag cgacccggcg gcgtaggcgg
     4381 caagcccccg ccagttgaac cggggaacgg ccgccccgtc gagcgggggg tagcggcccc
     4441 gatggccgac gaagaaatcg gccatgatca ccccgccgag cggagggatg agcaccccca
     4501 tcatgaccat gtaggggatc agccagtcat acatcccggc cagggcgagg agggttccgg
     4561 cggcggcgcc gccaaacgtg atcaaccgcc gtcgctcggt tcgccagaag tggcacccgg
     4621 cgaccgagaa gttgtagatg gtgttatcct gggtcgtcca gatgttcagg aagagcagca
     4681 gtataccggc aaagaggaga ccctggattt ccagaacctc gacgatatcg gagacgccgt
     4741 agatggcggc gctgacggcg ccgacggcga tcatcagccc gttaccgacg aagaaggcga
     4801 tgaggacgga gccgacggca acccggccgg acccggcaaa ccgtgtccag ttggcgacct
     4861 gcgtaccacc ggagacaaag gttccgacga cgatggtgag cgcttcggca acggtgagcg
     4921 tccctgccgc gaggggcccg gccgcgctcc ccgcatcacg gagagcaacc gcgatgctta
     4981 cagcgatgag gaggagcatg gccgggacgg cgatccacga gagggctgca agaccgcgac
     5041 agccgacgta tgctgtcatg cagaaaccga gaccgaagac gatcatcagg ggcgccgcgc
     5101 agcccgcatc gaggttgaga aggcgtacga gggcgatcgc gatcgtcgcg gtcccccagg
     5161 cataccaacc gagctgggtg aggccgagga gggtatcgga ccaccagctc ccgatatccc
     5221 cgaaggcaaa ccgggcggag aggacggtgg aaaaaccgct cctgaagccg atgtaaccga
     5281 gaccggcgac gtagacggcg aggatcgcgt ttccccagag gatgatccag acaaggtcgg
     5341 gccagaaccg gaacgcaacc ccgatactcg cgccgcccca catcgtggcg gagaagaagg
     5401 tgaacccgag caggaccagg gtgatcggcc agaattcctg ccgaccctcg tccggaacgg
     5461 cggagagcgg gtaatcctcc atgccgctcc tgcccctttc gtcgccgctc atcttctccc
     5521 ggttcaccgc catttcgccg atgctggagt gtctgccgcg caggactaat gcgttttggt
     5581 tcttcttccg ggtatccggc gcccggtcga tccagaggag tcatatacca ggccggccat
     5641 ctctcctgta tgccgcgagt gaggatcaat gagggagacc tggtctccgg ggccggcgtg
     5701 gaggggcgcc ggaagaagaa gattgatgag agcccggaac ctccccggga ggagcccgcg
     5761 gggaaggagc cggcgcccgt gaagaagggg gggcgcccca gctttgggga tgcggacggc
     5821 gccgacatat tcttcacgag aaaataggtt atttcagaac ccgtatatcg gcggcggagg
     5881 caaccaccac ctcccgctcc ccgcggtagg tctgcacgac cccgtagagc gctacgcttg
     5941 cgtttttgtc gagttcgagc ctggccgcca cgtcctgcgg caggaagacg gcggtcccgt
     6001 tgacggtgag gatccggtgc ccgccggtcg aggtcttccg gatctcctcg atcctcccct
     6061 caaggaggac gaacgcaccg tcggagactt cctcggagta cggtgccgcc acgagcggcc
     6121 gcccgacggc atcaaagagg aggtgtaccg caagcacgat cccgacgacg catatgagaa
     6181 cggcgagggc cgttttctcc tgccgttcca gcataggtac ccaatggatt tttgggaata
     6241 taacttccgt cactttgata tgttaacggc gttaactgat ctgtatggat gatgtgaacc
     6301 ttcctccgtc ctcaagaaag atcctcctgc ttctcgagga cgggggcgcc ctgacccaca
     6361 aggagctggt ccgtctcagc agtctggcac cccgcaccgt ccgctacgcc ttaaagaggc
     6421 tcaaagacaa tgatatgatc gtggaaaagt tcaacttccg cgacgcccgc cagatcctct
     6481 atgagtacaa aaactccccg atggtgtcgg cacaatgact gaacctccct cctgcgacat
     6541 catgcgttgc gatacgcttg cccggcggct cctcccccgg atgcgcgccg agatggtcta
     6601 ccgcctggtg aacgagcgcg gcatcagcca gagcgaggct tcaaaacgcc tcggcatcag
     6661 ccgtgcggcg atatctcagt acatgagccg gaaacgcgga tttacccggc aggacttccc
     6721 cggcgacctc gacgtcgtca tcgaacggtg ggtctccacc atcgtctcgg gcgaggggac
     6781 gatcaccatc tgcgacgtct gccgctcggc cggcctctcc ggccgatggt gattatcagg
     6841 tgcgccgccg ccggtagatg tagagcccca tgagcgcggc gaggatacat gccgccgcca
     6901 ccaccttgag gcgatccggc ccctccgcca cggtcccgtc ggcagctgcg gcgtcccaat
     6961 cgtccgcaaa gacccgggcg aagtaggcgg tgatatccgg gtgctcgatg atcacgcccg
     7021 cttcccggtt gaatgcaggc gagttggcgt tccagttgat gctgctgaca aggaccgccc
     7081 ggccgtcgac tatgacgccc ttgttatgca cttttgcgag gttgtttgcc gtgaggtcga
     7141 cgatccgcgc ctcgagcggc aacccctcgg ccgcggctat ccggttgatc aggtcgacca
     7201 tctcatcgtt gtccgcgtcg tcctcgatat tgaaccagca ggcatcgagg aggacccgca
     7261 ccgcgacccc gcgccgggag gcgtcgatcg cggccgcaag gtaggggttg agttcgtagg
     7321 tggtctcgtt cgttatgtag gcctgctcga tcgcgatgct ctcccctgcc ccctcgatca
     7381 gccggaggat gagggcgctc gtgtcggggg agaggaccgg ggtcacccgc gccccctccg
     7441 cgcggcaggg ggtgaactcc accgtgtagg cgggggcgta gggcatgcgg acgtcggccg
     7501 tcgtgccctc gagcgggacg atatccccgc cggagagatc aaagaggagg acctcccgga
     7561 agtaggccgc aagtttcggg tcatcgaggc atgcccccca cccgcggttg ccctgcagcc
     7621 ccggcgccgg atagccgccg ggcttgaagt tctcgctccc gatgaggacc gtcctcgcat
     7681 cgatgacgac atacttcgcg tggtcgtagc ggtacttcgc gtgggccgca tcggtcgtcg
     7741 tcatcgagag aaccacgatg ccgctccggt tgagtatgcc ggcgatcgcc cgctcctccg
     7801 gcggtatgcc gccgaccggt ccgccctcga ggaggacggt cacggccgca ccccgctcgc
     7861 gggcgcggat caggtcggca gccatctccg gatcggtgaa ctcgtagacg tttacgagga
     7921 tctcgcgctc agcagatgcg acggccgccg agaagacgtc atacgcgcaa tcgggggcga
     7981 cgaacgccgt caccgtgacg tcgtcgaacg tcgcggagga gaagcgggac tgcccgatga
     8041 agaacgggcg cgggtcccac acgccgtctt cgaggtagtg aacctgccct tcgcggggac
     8101 gaacgtcggc aggccaggcg acctgctgga cgatggtcgc cccatggtag aggtagagcg
     8161 cgtcggcctg gttcgccatc aggaggttcc cgctcgaggt gacgtccggc acggccgctg
     8221 tccgcttctc gatctcgaag tcgggcaggt agccgtgcgt ctgctcaaac gcgagactgt
     8281 ttcttgcgat caccagacgc ccgttgatcc aggtccccgg cgggaaccga aaaccgccct
     8341 tgccgtccga gacaacgaac ctatcaagcg atccttcgcc ctcgagcacc aggtactcgt
     8401 cgggatcacc gggccggtag gggtcagggc agaactcgac gatcaggata ccgccggccg
     8461 tgccggcaag gagacagagg agcaggatga tcgcagcgat ccgtcgcata cgcaaggtta
     8521 tgtattatcc ggataaagag caaacgatat gacaacgcca gcgcccccgc tcgtcgtcaa
     8581 ggtgggcggc agcctctttg accgggtagc cccgctgctc ggcatactcc gggaagtcgg
     8641 ccgtccggtg ctgatcgtcc ccggcggcgg gaagttcgcc gacctcgtcc ggcgccttgc
     8701 cgtctccgat acatccgctc actggatggc gatcgccggt atggagcagt tcgggtggta
     8761 catcgcctcg cacggcatcc cggcggtcgc cagccccgtc ctccccgcgg aagcgacggt
     8821 cctccttccc ta
//
LOCUS       DAOSEE010000090         8545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_3091_length_8545_cov_180.666784, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000090 DAOSEE010000000
VERSION     DAOSEE010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8545
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3091_length_8545_cov_180.666784"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PLI76_07645"
     CDS             <1..302
                     /locus_tag="PLI76_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOI61943.1"
                     /translation="HRCDGYSLEVVPFLDKTPRGIFATRAPRRPNAIGISILKLVAVD
                     GATLTVEDVDILDGTPVLDIKPYVPAFDAYPDERAGWLTTTSGAARTMRSDDRFC"
     gene            394..1221
                     /gene="modA"
                     /locus_tag="PLI76_07650"
     CDS             394..1221
                     /gene="modA"
                     /locus_tag="PLI76_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOI61944.1"
                     /translation="MTKKAIALITIVLIAGLLCTTGCTSQQTPATGDNTTPGATGTLL
                     FVYSGAGLKKPMNEIGAAFTEKTGIGVEYTFAGSGTLISQMDLSRKGDVFIPGGTPDY
                     RNARDKELVGEPGYVAYHVPIIVVQKGNPKNITSVDDFARPGLKIALGGANTTAIGRA
                     GDKLFEKHGILDAVEKNVVLRAPTINEVVVAMNMGTADAALITLDLVNPDTMDTIDLP
                     QEGGLTLVVPIGQTTFTTQPDAARQFVEFVTSDEGKAIFAKHGFPTYPDPAYAGIEP"
     gene            1218..1703
                     /gene="tsaA"
                     /locus_tag="PLI76_07655"
     CDS             1218..1703
                     /gene="tsaA"
                     /locus_tag="PLI76_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HOI61945.1"
                     /translation="MKESEAYSCRAVGVIRTPFSNPASTPIQSIFSTARGTVEIYPGF
                     RDGLIALAGFSHVILIYRFHRAASEELSERPLIDGAEPHGIFATRHFNRPNRLGLSVV
                     RVLGIDEGQISVEGVDLLDGTPVLDIKPYIPEFDSVPDARSGWVTPRHVEQIRRQSAS
                     F"
     gene            1715..2527
                     /locus_tag="PLI76_07660"
     CDS             1715..2527
                     /locus_tag="PLI76_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI61946.1"
                     /translation="MPPNRDALPRPSAFKALFLAVLFGLVAYMVVVIVGLVVYPPLPA
                     LIESLTSPEILFAIKLSLITSAISTTLCVVAAVPVAYSLARFSFPGRRLINSIFNIPL
                     ALPPLVAGVALLIFYGPSTFGRMLSATGLDVIYTPLGIIVAQFFVNLPYMVRVARSAF
                     ETINPRYEHVARTLGCTEWGAFRQVSLPLARSGIVAGLVITWSKSIGEFGAVLMLAGA
                     TQMKTETLPIALFLNMSTGDLDLAIAASVILIVISVASLAVFERYSGGRGVF"
     gene            2527..3576
                     /locus_tag="PLI76_07665"
     CDS             2527..3576
                     /locus_tag="PLI76_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI61947.1"
                     /translation="MLDIRGLSLSLGEFSLDGVDLTVADGEYFIILGPTGAGKTILLE
                     TIAGIYAPDSGTISLDGREITRTDPKDRGIGMVYQDYMLFPHLTVGENIGFGLRQQKI
                     EPDRIKERVSETANLLGIGHLLDRTPLTLSGGEQQRAAIARALVLRPRVLLLDEPLSA
                     LDAVTRDRLRRELKAVHRAMGTTIVHITHHFEDIFALADRVAVMQDGMIVQAGTPDEV
                     FRQPATEFVAAFTGMENIFPGISRVRGSEAAIDLGAITLRTVTAVEGDVYAGIRPEEV
                     IVSREAFESSAVNAFSGTVVEVQQNGIFSRVVVDAGVPFVAVLTRQSIARLGLAEGEP
                     AHVTFKASAVHVFSR"
     gene            3613..4134
                     /locus_tag="PLI76_07670"
     CDS             3613..4134
                     /locus_tag="PLI76_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61948.1"
                     /translation="MNAEDIQEEITFAAAEAALAVRFGIPAGALIPLLFSLRYGGDWS
                     YASGDVTALSAVTKTTVYDEASRTGYSLEEIYLFVDPVLLHREGTVHRLEKCGEAPAR
                     LLVARPYRVRLGARRVIRMTVHPLKREIRTEELDAREMAFSGSTAYGIDHELEHLAGR
                     EIAGEELRVFRFG"
     gene            complement(5072..5230)
                     /locus_tag="PLI76_07675"
     CDS             complement(5072..5230)
                     /locus_tag="PLI76_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOI61949.1"
                     /translation="MNSYRCTLCGYVYNPAIGDSANGIKPNTAFEDLPASWRCPRCGA
                     AKSRFVKV"
     gene            complement(5315..6874)
                     /locus_tag="PLI76_07680"
     CDS             complement(5315..6874)
                     /locus_tag="PLI76_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HOI61950.1"
                     /translation="MELAIGKAGDREIAVNAQELVTGRTCIIAQSGAGKSWGIAVLCE
                     RLLEAGIGFCLIDTEGEYFSLKDRFSLLWIGSADGCDAEIGKADIRSLMQDAICSRTA
                     VIFDVSEIDMRDEVAKFAEILYDLESRLKQPYLLIVEEADKFIPQAGTSIKKIEEISR
                     RGRKRGLGLLVATQRPSLVAKNVLSQCNSQIIGKLSIENDLKAVSLFFSSRKEVEELA
                     ELEPGEFFVMGSLSREKVRMRFGARVTKHRGVTPRLVAAPPARAGPCPCPREDPADPP
                     PAGNAVVPVLLREEALDIAKGKRRRRFRVLAPEERIVSGERVYRPLHRVEVRYIGGLI
                     RKATKTASFVIDGLTGCIVEVDRGLKVRPGFSELLGLDEAAVRIVANLANGGSTLVEI
                     EADTLLSPGVVKKAIKSLEAAKLVTTMKTVGDTVVYVPLLATEVPGLATLRPGADLPV
                     EPLREAPLEEKVTESSLRTILKGLEPTAEIVGFDTIYYPVYRIGFASEHGERAIILDG
                     CTGRELSFPDP"
     gene            complement(6958..8421)
                     /locus_tag="PLI76_07685"
     CDS             complement(6958..8421)
                     /locus_tag="PLI76_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI61951.1"
                     /translation="MPLSAPAANTLVTTPGELLLSGEARGTTMEQEEGHSYSRRFILV
                     LITIATFLNPFTGTAINLALPSIGAEFAADAATLAWVSSAYLLASVIFLLPGGRLGDS
                     RGRVTVFMAGIVVYTAGALLTIFTPTIGMLLAFRFLQGVGGAMIYANSVALITHLYPP
                     GERGYAIGLNTTAVYAGLSLGPFLGGALTQFLGWRSIFIVTALLAVPVLLYAGKFPAF
                     LNDRHRERFDVPGLVLSSALIFCLFLGLASATTPAGMVLLAAALVLGVVFYLVERRHP
                     NPLLPVSLLTSNRVFAASNAAALINYSATYAIAFLLSLYLQYIRGYEPVTAGTLLLVQ
                     PIVQVFVAPLSGRLADRMQPGQIASVGMAISAAGLFCLSMLDATTSVGVILALLVLLG
                     VGVGLFSSPNTTAIMASVEMRFYGSASAMTAMMRSLGMMLSMGAVLVVFAVIMGSTAV
                     TPAIFPEFLRSLHLIFLAFGILSAFGVVLSLLRNRLR"
BASE COUNT         1524 a         2916 c         2649 g         1456 t
ORIGIN      
        1 tccaccgctg cgacggctac tcgcttgagg tggtgccgtt cctcgacaaa acaccgcgtg
       61 gcatctttgc gacccgggcg ccccgacgcc cgaacgcgat cgggatctcc atcctcaaac
      121 tggtcgccgt cgacggagcg acgctcaccg tcgaggacgt ggacatcctg gacggcaccc
      181 cggtcctcga tatcaagccc tacgtcccgg cgttcgacgc ctaccccgac gagcgcgccg
      241 ggtggctgac cacgacatcg ggggccgccc gcaccatgcg gtctgacgat cggttctgtt
      301 aatataggag tgtattaagt taacaatatt tacataacca attagtacaa tatagtactt
      361 taatatcgcc gaagtggata ttgaacggta tccatgacaa aaaaagccat agcactgatt
      421 accatcgtcc tcatcgccgg cctcctctgc acgacgggat gcacatccca gcagacgccg
      481 gccaccggcg acaacaccac ccccggtgcg accggcacct tgctcttcgt ctactccggc
      541 gcaggcctca agaaaccgat gaacgagatc ggagcggcgt tcaccgagaa gaccggtatc
      601 ggcgtcgagt acaccttcgc cggttcgggc acactcatca gccagatgga cctctcccgg
      661 aagggcgacg tcttcatccc cggcggaacg cccgactacc ggaacgcccg ggataaggag
      721 ctggtcggag aacccggata cgtcgcctac cacgtcccga tcatcgtcgt ccagaagggg
      781 aaccccaaga atatcacctc ggttgacgac ttcgcccggc cggggctgaa gatcgcgctc
      841 ggcggcgcga acaccaccgc gatcggcagg gccggcgaca aactcttcga gaagcacggc
      901 atccttgacg ccgttgagaa gaacgtcgtc ctccgggcgc cgacgatcaa cgaggtcgtg
      961 gtggcgatga acatgggcac cgcagatgcc gcgctcatca cgctcgacct ggtgaacccg
     1021 gatacgatgg acaccatcga cctcccgcag gaaggcggcc tcaccctcgt cgtcccgatt
     1081 gggcagacca ccttcacgac gcagccggac gccgcccggc agttcgttga gttcgtcacc
     1141 tccgatgagg ggaaggcgat cttcgcaaag cacgggttcc cgacctaccc ggacccggcg
     1201 tacgcgggga tcgagccatg aaggagagcg aggcctactc ctgcagggct gtcggggtta
     1261 tccgcacccc tttttcgaac ccggcctcca ccccgatcca gagcatcttc tccaccgccc
     1321 gcgggacggt tgagatctac cccgggttcc gggacgggct catcgcactc gcggggttct
     1381 cccacgtcat cctcatctac cggttccacc gggctgccag tgaagaactc agcgagcggc
     1441 cgctcatcga cggcgcggag ccgcacggca tctttgcgac ccggcacttc aaccgcccga
     1501 accggctcgg gctatcggtc gtcagggttc tcgggatcga cgaaggacag atctccgtcg
     1561 agggcgtcga cctcctggac gggacgccgg tcctcgacat caagccctac atccctgagt
     1621 ttgacagcgt ccccgacgcg agatcagggt gggtgacgcc ccggcacgtc gaacagatca
     1681 ggcggcagag cgcatcattc taggttggtc ggccgtgccc ccgaaccggg atgccctccc
     1741 ccgcccctcc gcttttaagg cgctctttct cgcagtcttg ttcggtctgg tcgcctacat
     1801 ggtcgtcgtc atcgtcggcc tcgtggtcta cccgccgctc ccggccctca tcgagagcct
     1861 cacctccccc gagatcctct ttgccatcaa actctcgctc atcacatcgg cgatctcgac
     1921 caccctctgc gtagtcgccg ccgtcccggt cgcctactcc ctcgcccggt tctcgtttcc
     1981 gggcaggagg ctcatcaaca gcatcttcaa tatcccgctt gccctcccgc cgctcgttgc
     2041 gggcgtggcg ctgctcatct tctacggtcc ctcgacgttc ggacggatgc tctcggccac
     2101 agggctcgac gtcatctaca ccccgctcgg gatcatcgtc gcccagttct tcgtcaacct
     2161 cccctacatg gtcagggtcg cccggtctgc cttcgagacg atcaacccgc gctacgagca
     2221 cgtcgcccgg acgctcgggt gcaccgagtg gggggcgttc cgccaggtca gcctccctct
     2281 tgccaggagc ggcatcgtcg ccggtctcgt catcacctgg tcaaagtcca tcggcgagtt
     2341 cggtgcggtc ctcatgctcg cgggagccac ccagatgaag accgagaccc tgcctatcgc
     2401 gctcttcctg aacatgtcga ccggagacct cgaccttgcc atcgccgcgt cggtcatcct
     2461 gatcgtcata tcggtggcgt cgcttgcggt ctttgagcgc tacagcggcg gccggggggt
     2521 cttctgatgc tcgatatcag ggggctctcc ctctccctcg gggagttctc cctcgacggt
     2581 gtggacctga ccgtcgccga cggcgagtac ttcatcatcc tcgggccgac cggcgccggg
     2641 aagacgatcc tcctcgagac gatcgcgggg atctacgcgc ccgattcggg gacgatcagc
     2701 ctcgacggcc gcgagatcac ccgcaccgac ccgaaagacc gggggatcgg catggtctac
     2761 caggactaca tgctcttccc ccacctcacc gtcggggaga atatagggtt tggcctccgg
     2821 cagcagaaga tcgaacccga ccggatcaag gagcgcgttt ctgaaaccgc aaacctgctc
     2881 gggatcggtc atctccttga ccgcaccccc ctgacgctct ccggcgggga gcagcagcgg
     2941 gcggcgatcg cccgggcgct cgtcctccgg ccccgcgtcc tgctccttga cgaaccgctc
     3001 tcggcgctcg acgccgtcac ccgggaccgg ctcaggagag aactcaaggc cgtccatcgt
     3061 gcgatgggaa cgacgatcgt tcacatcacc caccatttcg aggatatatt cgccctcgcc
     3121 gaccgcgtgg cggtgatgca ggatggaatg atcgtccagg cgggcacgcc ggacgaggtc
     3181 ttccggcagc cggctacgga gttcgtcgcc gccttcaccg gcatggagaa catattcccc
     3241 ggaatatcac gggtccgggg cagcgaggcg gcgatcgacc ttggcgccat caccctcagg
     3301 accgtcaccg ccgtcgaggg cgacgtctat gccggcatcc ggcccgagga ggtgatcgtc
     3361 tcccgggagg ccttcgaatc gagcgccgta aacgccttct ccgggacggt cgtggaagtc
     3421 cagcagaacg gcatcttctc acgggtcgtc gtggatgccg gcgtgccgtt cgtcgccgtc
     3481 ctgacccggc agagcatcgc ccgcctgggg ctcgccgagg gggaaccggc ccacgtcacc
     3541 ttcaaggcct cggcggtcca cgtcttttcc cggtagcggg aggggccacg tacccggccg
     3601 caggatacat cgatgaacgc cgaggatatc caggaagaga tcacctttgc cgccgcagag
     3661 gccgccctcg ccgtccggtt cggcataccc gccggcgcgc tcatccccct cctcttctcg
     3721 ctcaggtacg gcggggactg gagttacgct tcaggggacg tcaccgctct atcggccgtg
     3781 acgaagacga cggtctacga tgaggcgagc cggaccggct actccctcga agagatctac
     3841 ctcttcgtcg atccggtcct cctgcaccgg gaggggacgg tccaccggct cgagaagtgc
     3901 ggggaggccc cggcgcggct gctggtcgcg cggccctacc gggtgcggct cggcgcccgc
     3961 cgggtgatca ggatgaccgt tcacccgctc aagcgtgaga tccggaccga agaactcgac
     4021 gcgagggaga tggccttctc gggctccacc gcctacggga tcgaccacga gctcgaacac
     4081 ctggccgggc gggagatcgc cggagaggag ctccgggtct tccggttcgg gtgagcgcct
     4141 tcgcgcatct cgcgcgaggc tgcatcgccc atccgcaccc gttaactctc gtggtgccgc
     4201 gggagttcgc gaaggccggt agatgggttt gcaactcccc atcaagacaa cgcgctcccg
     4261 ggccccctga gtgcatcgat cccaaacgaa cagaccatat ccggccttcg tcccatcccc
     4321 ccacggagcc tcccctccgt ctcccccacc ccacccaggc ttcacccagg tggcataccc
     4381 atacgcttct tcggtagccc ccaccccacc cgggcttcac ccaggtgaca tacccatacg
     4441 cttcaccggc aacccccacc ccgcccgcgc ttcgcgctcc tcccccgccc cctccgcagg
     4501 aatagttctg gcagcggttt cccgggggag gagagagcca gagcccggtt ttccaggctt
     4561 gtcccggtac actggaccgt ggtggatatc gccacggggg gaggggaccg gggagggggc
     4621 tcgtcccctc cccgcaggtg ggtacccgta tgcttcttcg gcaaccccca ctccgcccgg
     4681 ccttcggcct cctcccccgc cccggggggc gggggcagtg cgtggcgata gccagtggga
     4741 agccgtgcca ggggatggtg ctggcggcgg tttttcggag gggcgggcag agaagaacct
     4801 gctacggact tttcccgtcc cgagccccac aacccccgga tcggcccgcc ggtcccgtac
     4861 ttgcttgaat gccggcggat ttcgtgatat tttccggtca ccgggttgag atccgctcct
     4921 ttccggcagg ctgtgtcgac atgccatctc tccccgcgca gcccgtatat cctcagaaaa
     4981 gtccttcttg ctgcaggcca cgcccgcggt cggcgactcc cgggcagatc ccggcgcccc
     5041 tgaaagaaaa gattggttgt gagggttccc ctcagacctt cacgaaccgg ctctttgccg
     5101 cgccgcagcg ggggcatcgc cagctcgccg ggaggtcttc gaatgctgta ttcggtttaa
     5161 tgccgttcgc agagtccccg atcgcggggt tgtagacgta gccgcagagg gtgcatcggt
     5221 atgaattcat aactggtact cctcttctcc ccggccgcga ggccgacggg gttgtacgat
     5281 ctcatttgtc ccggggcagg gataagcgct ccggtcaggg gtcgggaaaa gagagttccc
     5341 gcccggtaca gccgtcgagg atgatcgcgc gctccccgtg ctccgacgcg aaccctatcc
     5401 ggtagaccgg gtagtagatc gtatcgaacc cgacgatctc cgccgtcggc tcaagcccct
     5461 tcaggatggt ccggagcgag gactccgtga ccttctcctc aaggggcgcc tcccggagcg
     5521 gctccaccgg gagatccgcc cccggccgga gggtggcgag ccccgggacc tccgtcgcga
     5581 gcagggggac gtagaccacc gtatcgccca ccgtcttcat cgtggtgacg agtttcgccg
     5641 cctcaagact tttaatcgcc ttcttcacca cgcccgggga tagaagggta tcggcctcga
     5701 tctcgaccag tgtcgagccg ccgttcgcaa gattggcaac gatcctcacc gcggcctcat
     5761 cgagccccag gagctccgag aaccccggcc ggaccttcag cccccggtcc acctcgacga
     5821 tgcagccggt aagcccgtca atgacgaacg acgccgtctt cgtcgccttc cgtatgagcc
     5881 cgccgatgta gcggacttcc acccggtgaa gcggccggta gacccgttcc cccgagacga
     5941 tccgctcttc gggcgcgagg acccggaacc ggcgcctgcg cttccccttc gcgatgtcga
     6001 gcgcctcctc gcggagcagg accgggacga cggcattccc ggcgggcggc gggtccgccg
     6061 gatcctcccg ggggcagggg cacggtccgg cccgggccgg cggcgcggcc acgagccggg
     6121 gcgtcacccc ccggtgcttc gtcacccggg ccccaaaccg catccgcacc ttctcccggg
     6181 agagagagcc catcacgaag aactccccgg gctcaagctc cgcgagctct tcgacctctt
     6241 tgcgggacga aaaaaagagg ctgaccgctt ttagatcgtt ctcgatcgag agcttcccga
     6301 tgatctggct gttgcactgc gagaggacgt tctttgcgac aagcgacggt cgctgcgtcg
     6361 cgactaagag accgagcccg cgtttgcgcc cccggcggga tatctcctcg atcttcttga
     6421 tcgaggtccc cgcctggggg atgaacttgt ccgcctcctc cacgatcagg aggtacggct
     6481 gcttcaaccg gctctccagg tcgtagagga tctctgcaaa cttcgccacc tcatcacgca
     6541 tatcgatctc cgagacgtcg aagatcaccg ccgtccgtga gcagatcgcg tcctgcatca
     6601 ggctccggat gtccgccttc ccgatctccg cgtcgcagcc gtcggccgaa ccgatccaga
     6661 ggagggaaaa ccggtccttg agcgagaagt actcgccctc cgtatcgatg aggcagaacc
     6721 cgatccccgc ctcaaggagc cgctcgcaga ggaccgcaat cccccagctc ttcccggccc
     6781 ccgactgcgc gatgatgcag gtccgtcccg tgaccagctc ctgtgcattg acggcgatct
     6841 cacggtcgcc ggcctttccg atggcgagct ccatctgatc agatgatcac aggatgcccc
     6901 gaggtttaac gtttcccatc ggtttccaag agagatatag ggagatcagg cggatattta
     6961 ccgcaatctg ttccggagga gcgataggac gacaccgaaa gccgagagga tgccgaacgc
     7021 gagaaagatc aggtgcaggc tcctcagaaa ctcaggaaat atagccggtg tgacggcggt
     7081 cgaccccatg atgaccgcaa agaccacgag gaccgccccc atgctcagca tcatgccgag
     7141 cgagcgcatc atcgccgtca tggccgatgc gctcccgtaa aatctcatct ccacagaggc
     7201 catgatggcg gtggtgttcg gagacgagaa gaggccgacc cctacgccca ggaggaccag
     7261 gagggcgagg atcacgccga ccgatgtcgt cgcatcgagc atcgagaggc agaagaggcc
     7321 cgcggccgat atcgccatcc ccaccgaggc gatctgtccg ggctgcatcc ggtcggcgag
     7381 acggcccgat agaggggcga cgaagacctg gacgatcggc tgtacgagga ggagagtgcc
     7441 ggcggtgacg ggttcgtagc ctcttatgta ctggaggtag agggagagga ggaacgcgat
     7501 cgcgtaggtg gcgctatagt tgatcagggc agcggcgttc gatgcggcaa agacccggtt
     7561 cgatgtaagc agcgagaccg ggaggagggg gtttgggtgc cgccgctcca ccaggtagaa
     7621 gaccaccccg agcacgagcg ccgccgcgag gagcaccatg ccggccgggg tggtcgccga
     7681 ggccagcccc aggaagaggc agaagatcag ggcggaggag aggacgaggc ccgggacgtc
     7741 gaagcgctcc cggtgccggt cattcaagaa cgccggaaac tttccggcgt agaggaggac
     7801 cgggacggca aggagcgcgg tcacgatgaa tatgctccgc cagccgagga actgggtcag
     7861 ggcgccgccg aggaacggcc cgagggagag cccggcgtag acggcggtgg tgttgagccc
     7921 gatcgcgtag ccccgctcgc cgggcgggta gaggtgggtg atcagggcca cgctgtttgc
     7981 gtagatcatg gcgccgccga ccccctgcag gaaccggaag gcgaggagca tgccgatcgt
     8041 gggcgtgaag atggtgagga gagctccggc ggtgtagacg acgataccgg ccatgaagac
     8101 ggtgactctc ccccgggagt cgccgagcct ccccccgggg aggaggaaga tgaccgacgc
     8161 aaggaggtag gcgctcgaga cccaggcgag cgtggcggcg tcggcagcga actcggcccc
     8221 gatggagggc agggcgaggt tgatcgcggt gcccgtgaac gggtttaaga acgtggcgat
     8281 cgtgatgagt accaggatga atcttcgcga atacgagtgc ccctcttcct gctccatcgt
     8341 cgtccctcgt gcttccccgg agagcagaag ctcgcccgga gtcgttacaa gggtatttgc
     8401 agcgggcgca gataagggca ctgatgctcg cctgtgcagt acattaatgc gaacggctcc
     8461 ctaagaccct cacgcgtatg cccgaccctt ccccggagac ccggcaggag gtccgcgacc
     8521 tctcgatccg gctcatcctg ctgct
//
LOCUS       DAOSEE010000091         8350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_3182_length_8350_cov_203.232429, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000091 DAOSEE010000000
VERSION     DAOSEE010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8350
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3182_length_8350_cov_203.232429"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..5784)
                     /locus_tag="PLI76_07690"
     CDS             complement(<1..5784)
                     /locus_tag="PLI76_07690"
                     /inference="COORDINATES: protein motif:HMM:NF023252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3344 domain-containing protein"
                     /protein_id="HOI61952.1"
                     /translation="MIGLAAPAAAAGTVGGVQLVDLAVPFIENQEQLPDEVRYYANTF
                     YGTAYVTDTDLTHAVTVGTEDGTQGVALKERFLNRAGSAISFTPSGEEPADTQASYFI
                     GNDPAGWQTGLATYNLVSLGEIYPGITVKAKARSVNIEKLFYVAPGAAPDEIRIEILG
                     AESLRLADDGSLVVVTGAGEVSLTAPEAFQDGHGVAVAYRVLGDDTYGFEVGEYDTGR
                     ALLIDPTLAYSSHLGGSDRDYGESIAVDGSGNAYIGGYTKSSDFPVTAGAIQAIKAGS
                     SSSYDAFVAKVNADGTGLSYCTYLGGTGKENCYGIAVDGDGNAYITGSTGSSDFPATA
                     GVFQTANAGGSDVFVTKINSSGTGLVYSTYLGGAGADTGYGIAVDGDGNAYITGSTGS
                     SDFPVTAGVFQTANAGGSDVFVTKMNSNGTNPVYSTYLGGIGGTEQGSGIAVDGSGNA
                     YVGGYTLSADFPVTTGTVQSTYGGGMYDGFVTKVSPGGSQLTYSSYFGGSESDQVNGI
                     AVDSAGTAYITGQTNSDNIPVTDGAFQATRNSPDNAEAFVARILQDGSGLLYSTYLGG
                     SGADGASSIDVDGAGNAYVVGQTNSNNFPVTGDAFQTAHAGSFDAFVAGINSDGSTLD
                     YATYFGGSSGNDYGQSIAMDDSGNLYVAGYTTSSVPVTDGAFQASSGGSYDAFVAKFS
                     GSAPVLTTIEVSPATANLAVDATQQFTAVAKDQNDDEMTGVTFDWSSGNETAGTVNTT
                     GYFTALAAGTTTVTAAAGSVTGTAEVTVTPAAQIDLNITGVVNPYPGVAVFAREPNTV
                     QVRGIKNAGTDTVTNIVVALYVSDVFEGTVPVNTTTIESLAGGAQIAITTIVDPTIRN
                     LQGGTVRYTAIVDPDNLIAETDEANNVKVGFAAKPVLYNGYKGKMYWEGGSNVTTVRT
                     YDLRGDIIHSFGDSQYMSGSFGSEGWTNYTVTWTAGDLPLPEGAAVHDAWLYVPYCWD
                     NTNSAPDNVSIDFNGVRIPYVNWYNDVSNFGAYADHYYGLLTYNVTDLYQTGANNTAL
                     FARTDTNAKISPAGFTLAVIYEDTSAARKQIFVNEEFDILGADEGNYGTSMAEATAYV
                     PFSGAIIDTGSVARANLTTFVPWGNDEGNLFFNGEQIGTQVWSHGGSGATGEVGGTQV
                     AVDEREVTAYLNATGNEAAIQSDDTWKSPVMVAGLTFLVVEYADDSGGDAPVAAFTAT
                     PLNGLAPLAVTFADASTNSPTAWSWESRPSGTTENWTLFSTDQSPTHTFAAGAYDIRL
                     TATNANGNDTLTKTQYISSSAGAKRLATVQSGTVSGDLYVGAYGGWSSGISYATNTFN
                     QTFTLPAYTDIQWARLYTVVYAAGTDSRAGTVTVRFDGNGTYATLGTETLATAGDNMA
                     NVYPVNDHVNRQYSDYLLWYDVTDLIGSEDPKAEVVATPVAPNFDGRIKELVLVVAYN
                     DGDGDQVHYWVNEGHDWQANGADGVTSTFATGGLGAGWTDATLRNVMLSSKDALYTFN
                     GAPCTGADSSITFRANAWDVRTNLTAGSESSLTYVPNGGSYKTTLATLAVRYPASVAL
                     PDLTVSTLAPNSGEVFSASHNTYTATVTNIGTADAGAFAVGFNASGAAGTVPVTGLAA
                     GATATVTWTDETIRNAGDVVTITVTANPDGLITESNEANNIRTIDKTVVHNGYRGMRW
                     TDGGDIATAVTYDIRGDLLWSAGNSTYLSVSTYPNWTDYIVAWTAGDLAVPEGATIAG
                     ARLYVPYTWDKGPIFPSNVTLAFNSETVDRSAFYEDEKMWGSSYPYGMVVYDVTDQFS
                     AEGNTALLANTFPGGGNVSVRGMLLAVVYDDGTTAPHTVLMNEGFDLLYGGEAQGTTP
                     EQATAYAPFSGVETDDVLSATLVTVAPGAGPTKGDLLFNDQTWTRVWNYTGTTQIGVD
                     ERDVTTFLIDGENLVGFRSSADYMEAAA"
     gene            complement(6219..7142)
                     /locus_tag="PLI76_07695"
     CDS             complement(6219..7142)
                     /locus_tag="PLI76_07695"
                     /inference="COORDINATES: protein motif:HMM:NF023252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3344 domain-containing protein"
                     /protein_id="HOI61953.1"
                     /translation="MLVLMAALAMVAPAAADPYLGGDPLVTANGTNGIVTGGLWFDAY
                     PGFEFAYPTPIVHSFNPLPCDPEDIAWARLYVDTYIGNMQANYPLNTTVEFDGAGLGN
                     YDTLGIEIMNTTYTFPEGTGTGTIWINDHSTRVTSDHLMWYDVKDEITSSNVTARVHT
                     ARPDGTQPLDGRVKMIALVVAYDDGDFDKIRYWVNQGHDTDSYVTDDEGYSYVGETGF
                     DTSAVTAVDSATLTAIYLGSYNGNYAFESNALPWSNPQQGSYFGYQSWNVTSYVDTGG
                     ISTMTFDRNSTHSGQYSGYFKIPLALLTVEE"
     gene            7909..>8350
                     /locus_tag="PLI76_07700"
     CDS             7909..>8350
                     /locus_tag="PLI76_07700"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOI61954.1"
                     /translation="MPANFLDLHIVDYCQLACRHCYLHKGNTSMPLEMIREVSTNFLQ
                     ADLPLPQSTIILSGGEALLHPQFGEVCSIVRSLNGYIALSSNGILVPQYIGLFRRNDG
                     IQISIDGNSRVHDFIRGAGSYENAMRALRLLDENNIRHSISFTAN"
BASE COUNT         1554 a         2401 c         2564 g         1831 t
ORIGIN      
        1 cgcggcggcc tccatgtagt cggcgctgct ccggaacccg accaggttct cgccgtcgat
       61 gaggaacgtg gtcacgtcgc gttcatcaac cccgatctga gtcgtgccgg tgtaattcca
      121 gacgcgggtc caggtctgat cattgaagag gaggtcgccc ttggtcggtc cggcgccggg
      181 ggcgacggtg accagggtgg cactcaggac gtcatctgtc tcgacgccgg agaatggtgc
      241 ataggcggtt gcctgctccg gcgtggtccc ctgggcctca ccgccgtaga ggaggtcgaa
      301 accttcgttc atgaggacgg tgtggggggc ggtggtgccg tcgtcgtaga cgaccgcaag
      361 cagcatccca cggaccgaga cgttgccgcc gccggggaag gtgtttgcca gcagggcggt
      421 gttgccctca gcggagaact ggtcagtgac gtcgtagacg accatgccgt agggatacga
      481 ggagccccac atcttctcat cctcgtagaa ggcagaccga tcaacggtct cgctgttaaa
      541 ggccagggtg acgtttgagg ggaagatcgg acccttgtcc caggtgtagg ggacgtagag
      601 ccgggcgccg gcgatcgtgg ctccttcggg aaccgcgagg tcgccggcgg tccaggcgac
      661 gatgtaatca gtccagttcg ggtaggtgct gacggagagg taggtgctgt tgccggccga
      721 ccagaggagg tcgccgcgga tgtcgtaggt gacggcggtg gcgatgtccc cgccgtcggt
      781 ccagcgcatg ccgcggtagc cgttgtggac gacggtcttg tcgatggtgc ggatattgtt
      841 cgcttcgttc gattcagtga tgagcccgtc ggggttggcg gtgacggtga tcgtcacgac
      901 gtcgccggcg ttgcggatgg tctcgtcggt ccaggtgacg gtagcggtcg caccggccgc
      961 gaggcccgtg acgggaaccg tgccggccgc tccgctcgcg ttgaacccga ccgcaaacgc
     1021 gccggcgtcg gcggtgccga tgttcgtgac cgtcgccgtg taggtgttat gggatgcgga
     1081 gaagacctcg ccgctgttcg gcgcgagggt cgagaccgtg agatcgggga gggcgacgga
     1141 ggccgggtat ctcacggcaa gcgttgcgag cgtggtcttg tacgacccgc cgttcggcac
     1201 gtacgtgaga ctgctctcgc tgccggcggt caggttggtc ctgacgtccc aggcgttcgc
     1261 cctgaacgtg atcgatgagt ccgcacccgt acagggagcg ccgttgaacg tatacagggc
     1321 gtcttttgag gagagcatga cgtttcggag ggttgcatcc gtccagccgg caccaagccc
     1381 gccggttgca aaggtcgagg tcaccccgtc ggcgccgttt gcctgccagt cgtgcccctc
     1441 gttcacccag tagtgcacct ggtcgccgtc gccgtcgttg tacgcgacca cgaggacgag
     1501 ctccttgatg cggccgtcga agttcggtgc caccggggtt gcgacgacct ccgctttcgg
     1561 gtcctctgaa ccgatgagat cggtcacgtc gtaccagagc aggtaatcgg agtactggcg
     1621 gttcacgtgg tcgttgaccg ggtagacatt ggccatgttg tccccggcgg tggcaagcgt
     1681 ctcggtgccg agcgtggcgt atgtgccgtt gccgtcgaac ctgacggtca cggttcccgc
     1741 ccggctatcc gtacctgcgg cgtagacgac cgtgtacagc cgtgcccact ggatatcggt
     1801 gtatgccggg agggtgaatg tctggttgaa cgtgttcgtc gcatatgaga taccggagct
     1861 ccatcccccg tatgccccta cgtaaagatc gccggagacc gtgccgctct gcactgtggc
     1921 gaggcgtttt gcccctgcgg aggacgagat gtactgggtc ttcgttagag tgtcgtttcc
     1981 gttggcattg gtcgcggtga gccggatgtc gtaggctccc gctgcaaagg tgtgggtcgg
     2041 gctctggtcg gtggagaaga gcgtccagtt ctcggtggtc ccggacggcc gcgactccca
     2101 cgaccatgcg gtgggggagt tcgtcgaggc gtcggcgaag gtgacggcaa ggggtgcaag
     2161 cccattcagc ggagttgcgg tgaatgctgc aaccggcgca tccccgccgg aatcgtcggc
     2221 gtactccacc acgaggaaag tcagtccggc aaccatcacc ggcgatttcc aggtatcgtc
     2281 gctctggatc gctgcctcgt tcccggtcgc gttcaggtac gccgtcacct cgcgctcgtc
     2341 cacggccacc tgtgtgccgc cgacttcgcc ggtcgcgccg ctgccgccgt ggctccagac
     2401 ctgcgttccg atctgctctc cgttgaagaa gaggttgccc tcgtcgttgc cccatgggac
     2461 gaacgtcgtc aggttcgcgc gggcgacgct ccccgtatcg atgatcgcgc cggagaacgg
     2521 gacatatgcc gtcgcctcag ccatgctcgt gccatagttc ccctcgtcgg cgccgaggat
     2581 atcgaactcc tcgttgacga agatctgctt tctggcggcg gatgtgtcct cgtagatcac
     2641 ggcgagggtg aacccggccg gcgagatctt ggcgttggtg tccgtccgtg cgaaaagcgc
     2701 cgtgttgtta gcgcccgtct ggtagaggtc ggtcacgttg taggtgagca gaccgtagta
     2761 atggtcggca tacgccccga agttcgagac atcgttgtac cagttcacgt aggggatccg
     2821 gacgccgttg aagtcgatcg agacgttgtc gggtgcagag ttggtgttgt cccagcagta
     2881 gggcacgtag agccaggcat cgtgaaccgc cgcgccttca gggagcggca ggtctcccgc
     2941 cgtccaggtc acggtgtagt tcgtccagcc ctcgctgccg aaactgccgg acatgtactg
     3001 gctgtcgccg aaggagtgga tgatgtcgcc gcgaaggtcg taggtccgca ccgtggtgac
     3061 gttgctgccg ccctcccagt acatcttgcc cttatagccg ttgtaaagga cgggtttagc
     3121 ggcgaaaccg acctttacgt tgttggcctc gtcggtctcg gcgatgaggt tatcggggtc
     3181 gacgatggcg gtgtatctca cggtgccgcc ctgaaggttg cggatcgttg ggtcgacgat
     3241 ggtggtgatg gcgatctggg ctccgcccgc aagagattcg atggtcgtgg tgttgaccgg
     3301 gacggtacct tcgaagacgt cgctcacgta cagggcgacc acgatattgg tcacggtatc
     3361 agtcccggcg tttttgatgc ccctgacctg aaccgtgttt ggttcccttg caaagaccgc
     3421 gacgccggga tacgggttca cgacgccggt gatgttgagg tcgatctggg cggccggagt
     3481 caccgtcacc tctgccgtcc cggtcacgct ccctgcagca gcggtgacgg ttgtcgttcc
     3541 tgccgcaagg gcggtgaagt acccggtcgt gttcaccgtg ccggcggtct cgttgccgct
     3601 cgaccagtcg aatgtgacgc cggtcatctc atcgtcgttc tggtcctttg cgacggcggt
     3661 gaactgctgg gtggcgtcga ccgccaggtt cgccgtcgcg ggggagacct cgatggtcgt
     3721 caggacaggg gcagaaccgc taaatttcgc tacgaaggcg tcgtaactgc cgccgctact
     3781 cgcctggaac gcaccgtccg tcaccgggac ggacgatgtg gtatatccgg cgacgtagag
     3841 atttccggaa tcatccatcg caatgctctg cccgtagtca ttgccactgc ttcccccgaa
     3901 gtaggttgcg tagtcgagcg tggaaccatc cgaatttatt ccggcaacga atgcatcaaa
     3961 tgatccggca tgggctgtct ggaaagcgtc gcctgttacg gggaaattat tggagttggt
     4021 ctggcctacc acataggcat tccctgcacc gtccacgtcg atagaacttg caccgtcagc
     4081 gccacttcct cctaggtagg tcgagtacag gagcccggaa ccgtcctgga gtatccgggc
     4141 aacgaaagcc tccgcattgt cgggactgtt tctggtcgcc tggaaggcgc cgtctgtcac
     4201 cggtatgttg tcggagttgg tttggccggt gatatacgcg gtacctgcgg agtccaccgc
     4261 aattccattg acctgatcac tttcgctccc tccgaagtag ctggagtacg tgagctggga
     4321 tccgcccgga ctcaccttgg tgacaaatcc gtcgtacatc ccccccccat aggtcgactg
     4381 aacagtgccg gttgtaaccg ggaagtcggc ggataacgtg taaccaccaa catacgcgtt
     4441 tcctgacccg tcgacggcga tcccggaccc ctgctcggta ccgccgatac ccccgaggta
     4501 tgtggagtag acaggattgg taccgttgga gttcatctta gtgacaaata catcgctccc
     4561 tccggcattg gcggtttgaa atacgccggc cgtcaccggg aagtcgcttg acccggtaga
     4621 cccggtgata taggcgttgc cgtcgccgtc caccgcaata ccataccctg tatctgctcc
     4681 ggctcctcca agatacgtgg agtagacgag gccggtgccg ctggagttta tcttggtgac
     4741 aaatacatcg ctccctccgg cattggcggt ttgaaatacg ccggccgtcg ccgggaagtc
     4801 gcttgacccg gtagacccgg tgatataggc gttgccgtcg ccgtccaccg caataccata
     4861 acaattctcc ttcccggttc ctccgagata ggtgcagtag gaaagtcccg taccatcggc
     4921 gttcacttta gcaacgaatg catcatatga agaggaagaa ccggctttaa tagcctgtat
     4981 tgcaccggca gtcaccggga agtcggatga tttggtataa ccgccgatat aggcattgcc
     5041 gcttccatcc accgcaatac tctctccata gtcccggtca cttcccccga ggtgactcga
     5101 ataggcaaga gtcgggtcga tgagcagggc cctgcccgta tcgtactctc cgacctcaaa
     5161 cccgtaggta tcgtcgccca gcactcgata agcgaccgcg accccatgcc cgtcctggaa
     5221 cgcctccggc gcagtcaggc tgacctcgcc tgcaccggtg acgacgacga gacttccatc
     5281 gtctgcaagc cggaggctct cggcgccgag gatctcgatc cgtatctcgt ccggtgcggc
     5341 gccgggtgca acgtagaaga gcttctcgat gtttacgctg cgggccttcg ccttcacagt
     5401 gatgccggga tagatctcgc cgagcgagac gaggttgtac gttgcaaggc cggtctgcca
     5461 ccctgccgga tcgttcccga tgaaatagga ggcctgtgta tcggccggct cttcgcccga
     5521 gggagtgaag gagatcgcag atcctgcgcg gttaaggaac cgctctttca acgcgacccc
     5581 ctgcgtcccg tcctccgtgc cgacagtgac ggcgtgcgtc agatccgtat cggtgacgta
     5641 cgccgtaccg tagaaggtat tggcataata cctcacttca tcgggcagct gctcctggtt
     5701 ttcgatgaac ggcacggcaa ggtccaccag ttggaccccg cccaccgtgc cagccgccgc
     5761 cgcgggcgcg gcgagcccga tcatgcacag cagtgcagcg atgctaaaaa tcaacaatgt
     5821 tcgttgcatt tttcgttcag ctccttcttt agcgttttat ctcgcccggc acgtcggcac
     5881 tgcgccccgg cgatcgggag agcgtcggta gtcaaatcgg acgatcagaa gatgacattt
     5941 agtactatat taagtgagat cattcatact cgatcatcac ttttataaaa tatgtattaa
     6001 tttatgtata tgttttgaaa attcgtgtca cgaacgggtg gataatccag tctccgacca
     6061 gatgcagccg ggaatggagg agtcggtggt ctgtcggttc cccgtgcggg tcgaaaaaag
     6121 agggggtcaa cccgagaggt tgaccgccga ttctgcgcct tctgcctccg taagggagcg
     6181 acgcgccgcc acggtatcgc cgcagcgaaa ctgcgcgcct actcctccac cgtcagcagg
     6241 gcgagcggta tcttgaagta gccgctgtac tgcccggaat gagtggagtt ccggtcaaac
     6301 gtcatcgtac tgatgccacc tgtgtctacg taactggtga cattccaact ctggtacccg
     6361 aagtacgatc cttgctgcgg gttgctccag ggcagggcgt tgctttcgaa ggcgtagttt
     6421 ccgttgtaac tccctaggta gattgccgta agagtggcgg aatcaacggc cgtgacagcc
     6481 gatgtgtcga agccagtctc cccgacatag gagtatccct cgtcgtcggt aacataggag
     6541 tcagtgtcgt ggccctggtt cacccagtag cggatcttgt cgaaatcacc gtcgtcgtac
     6601 gccaccacca gggcgatcat ctttactctg ccgtctaacg gctgggtgcc gtccggtctg
     6661 gccgtgtgca cgcgcgccgt gacgttcgat gaggtgatct catccttcac gtcgtaccac
     6721 atcaggtggt cgctcgtcac ccgggtggag tgatcgttga tccagattgt cccggtcccg
     6781 gtaccctccg ggaaggtgta ggtcgtgttc atgatctcga tgcccagggt atcgtagttc
     6841 cccaaaccag cgccgtcgaa ctccacggtc gtgttcaggg ggtagttggc ctgcatgttc
     6901 ccgatgtagg tgtctacata gagccgcgcc caggcgatgt cttcgggatc gcacggcagc
     6961 gggttaaaac tatgcacgat cggagtcggg tacgcaaatt cgaaccccgg gtacgcgtcg
     7021 aaccagagac cgccggttac tatcccgttc gttccgttcg cggtgaccag ggggtctccg
     7081 cccaggtatg ggtccgccgc cgccggcgct accatcgcga gcgccgccat cagcacgagc
     7141 atgcccgtca gcatgcactg tttggctgtt ttcattttcc atctccatgt ttatttactg
     7201 tcgggggtac catccccggc agagggagat cgttttagta caataaatgt ctttttgttt
     7261 aatatctgat atgaatttta tttaattaat atttaataaa tagttgtaat tttaatattc
     7321 atgatatctc ttggtactgt ttggtatcgt cgcgggggga ctatgccctg tatgggtcaa
     7381 aacagccggg cgcgcgaaaa tctgtcggaa cccggtattt cgctaaacta tttgcgaaag
     7441 cctccgggag gccagataac ctactttcct cgcgcctttg gtgctcgagc tccgctccgt
     7501 aggaacatcg cacttcgaag atcttcacgt ccaatgcgtg aggcatcggt cctgccgcca
     7561 ccgcacgcga agcccggtgt cgtgtgttgc cgcctcctct tcgcacctga cggtgctctt
     7621 gtcctggacg ttccgtccat cacatatcgc tatgcctgtc cccagccccc cgctggatgg
     7681 tgtgggagtt gatgtacggg gcagagcaat gtgcgttgga ataggaggcc atcaaaccgg
     7741 agatacacat aattcgatga gctcggaaca taatcccgat gggcaactgt taagtaagat
     7801 caaaacaatc acaaaaattg ttaatactac ataattaagc gttacgaaat ctcgcgtgac
     7861 atgatattgt aatggaaaga aaatgtgccg aacagaatag tggtatgaat gcctgcgaac
     7921 ttccttgacc ttcatatcgt cgattactgc cagctggcat gcaggcattg ctatcttcac
     7981 aaaggaaata cctcgatgcc gctggagatg atacgggaag taagtaccaa cttcctgcag
     8041 gcagatcttc cactccccca gagtactatc attttgagcg gcggggaagc ccttctccac
     8101 ccgcaattcg gcgaagtatg cagcattgta cgaagtttga acgggtacat agccctgagt
     8161 tcgaacggga tccttgtccc gcaatacatt ggtctgttcc ggagaaacga cgggattcag
     8221 ataagcattg atggaaatag ccgcgttcac gatttcatcc gtggtgccgg gagctacgaa
     8281 aacgcgatga gagcactgcg gctactggac gagaacaaca ttcgccacag catctcattt
     8341 accgccaaca
//
LOCUS       DAOSEE010000092         8143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_3275_length_8143_cov_284.950420, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000092 DAOSEE010000000
VERSION     DAOSEE010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8143
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3275_length_8143_cov_284.950420"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /gene="gatB"
                     /locus_tag="PLI76_07705"
     CDS             <1..1107
                     /gene="gatB"
                     /locus_tag="PLI76_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOI61955.1"
                     /translation="VEIEDDEGHEKIVRITRVHLEEDPGRLVHVAGASKYSLVDYNRS
                     GIPLLEIVTEPDMRSPQEARRFLNKLRTILEYLGVFDGEREGALRVDANISIEGSARV
                     EVKNISSYKGVEKALTFEATRQRNLLRRGQTVARETRHFMEGRGITTSARGKEEAHDY
                     RYFPDPDLGPIRVADRVAAIDLPELPTARKNRFMAQYGISLNHARTLTGDPRLADFYE
                     HVAPADPILAATWAADILLGELNYRDMSIAAVPADRIRELLALLRAETITDAAGVQVL
                     REMLDACAAGRPCEKPADIVKREGLARATGDDFVAIVQAVIASNPQAVEDYRAGKKGA
                     LNFIVGQVMKETRGRADPRELGRIVSECIEKGGV"
     gene            1114..3909
                     /locus_tag="PLI76_07710"
     CDS             1114..3909
                     /locus_tag="PLI76_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HOI61956.1"
                     /translation="MHLIIAEKNISANRIAQILAGSEKVRASKDGSVSTYSFDTKTVV
                     GLKGHVVEVDFEPGYTNWRSEVHTPRSLIDAGIVKKPTEKKIVNLIQKLSKKADLVTI
                     ATDYDTEGELIGKEAYELVRAVNSTVKINRARFSAITPAEIRTAFENLTDLDFALAAA
                     GEARQTVDLMWGASLTRFISLAARRGGKDILSVGRVQSPTLAMIVDRERAIENFVPQT
                     YWMLSLLTEKDGVPVEARHTAGRFTDHDAALAAEAGTKEPLTVTDVKEGQKTDRAPTP
                     FDTTTFIVAASRLGLSAANAMRIAEDLYMNGYISYPRTDNTVYPKSLNLNGILETLRG
                     GVFDADVAWVQQHRRSVPTRGKKESTDHPPIHPTGAATREALGQDRWKVYELVVRRFL
                     ATLSPDATWTTTKCLFDASGEPYTATGGRLLSAGWRRIYPYSEAKENILPVFAVGERL
                     PIRKVNLEEKQTQPPARFSQSRLIQVMEELGLGTKSTRHEVIGKLISRRYVEGNPLRP
                     TLVGRAVTDALDNHAETITEPEMTRTLEEHMQLIKQRERSRDDVVDESREMLHRVFDK
                     LEAHEKEIGDEIMGQTAEEHTVGPCPACGHELRIRHLGASQFIGCTGYPECRFNISLP
                     GSTWGKAIRTDEVCKTHKLTHVRLIRKGSPPWTIGCPLCSHIASNIEALRMMPSMTDS
                     LVERLHAHHIYTVSEIAGMQPHDLAGAAGVGAEEAGQLIREAGGALDILRRRSELRKF
                     IRKIVPPKKGRSHAKITKSLVEQGVGDIRALSRSDSAVLKKAGIGETPAAELLAAARG
                     LCNERTLREAGIPAVSLKKYQAGGIASPDDLCYLPVPYLANKTGINPETVHKHADMVC
                     RHLGRPAPEKVTKTALERGRKELLAVPGIGEATVEKLYLAGIYDAATLREADPGEVSS
                     ITGIAKTRIHDYISHLK"
     gene            3914..4606
                     /locus_tag="PLI76_07715"
     CDS             3914..4606
                     /locus_tag="PLI76_07715"
                     /EC_number="2.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerol geranylgeranyltransferase"
                     /protein_id="HOI61957.1"
                     /translation="MHANWKTWAHVTKLDPDKRLPPGAAEEIATSGTDALMLSGTLNV
                     TQENLNELLDQVSPYGLPLVVEPASPDCAIFDGRIDHLFVPSVVNANDVRWIVGKHYT
                     WLRHANSIDWDMVVPEAYIVLNPNSAVGRVTGADCALSGRDVAAFVEVADRYFRFPIV
                     YIEYSGRYGDPSIVQAASDAVEHAVLYYGGGIRSAEQAAEMGRIADTIVVGNAVYDEG
                     IDVLRATVRAVQ"
     gene            4610..5308
                     /locus_tag="PLI76_07720"
     CDS             4610..5308
                     /locus_tag="PLI76_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOI61958.1"
                     /translation="MPEVSIVLVEPLYEGNVGFTARVMKNFGFTRLVLVNPCALGDDA
                     IMRAAHARDVLDGARRMTIDDVYREFDFVVATTGEVSKSVCTPMRMPYYAPAEVREAV
                     ADIDGTVAILFGRENWGLANTELARANFICTIPTSEAYPILNLSHAVGILCYELANLP
                     RGTYLLAGQVEMESLYRHIETFLDRIDHPDFKRENTMVLLRRVLGRTKLTVREVSTMH
                     GLMRRTEWHLENKE"
     gene            5332..5889
                     /gene="dcd"
                     /locus_tag="PLI76_07725"
     CDS             5332..5889
                     /gene="dcd"
                     /locus_tag="PLI76_07725"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HOI61959.1"
                     /translation="MILVDWQIEDRIRRGYIRVDPYDPALIQPNSLDIRLGSHFVWYV
                     PGDIVIDPYERESVCADVEEVVADSIVLAPGQFLLAKTLETIELPDDVVASIEGKSSI
                     ARLGIELHQTGGWIDAGFQGTITLEMCNVNSRPVRVYAGMPIGQLVFYRTDRAARPYN
                     MKKDAKYMGQQQATLSRYHENARHA"
     gene            6027..7862
                     /locus_tag="PLI76_07730"
     CDS             6027..7862
                     /locus_tag="PLI76_07730"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOI61960.1"
                     /translation="MRLLLIHSDHIEYEARKKTKVAEEDAVPKDALDEALAVFCAVES
                     VDEENIGDAVRQAADEIVTTARQLGTTNIMIYPYAHLSSDLASPEAAVSALRGIEEAL
                     SGESDFVVKRAPFGWYKAFSLSCKGHPLSELSRTIVPGEGTAAPKKEIEHEFFVFTPE
                     GERKDAAAYVDEKTPFAALIRKELGYPGPEGVEPVHVDLMRAKELVEYEPRSDVGHHR
                     WMPRGKLIRDLLTDYVLTQVLEYGGMPVETPVMYDLGDQAIAEHAAKFGERQYRFKSG
                     NRDMMLRFAACFGMFSIMHDMHISPNTLPMKLYELSTYSFRHEQKGEVIGLKRLRAFT
                     MPDMHTLCRDVDDALASFEEQLAMGWKSGEDLETPLVGVFRCTRDFFDQYELWVKQLV
                     AKSGVPMLIEVLSDRTHYWIAKVDLAAIDAQGRPIENPTVQIDVESADRFDIKYYTPE
                     GVEVHPPILHCSPTGSIERVICALLENTAQMEVPSLPTWLAPTQVRLVPVAGRHVCFA
                     EEIGARLNAAGIRADVDDRDESVNKKIREAGMDWAPYVAVIGDQEAETGRLTVTIRKL
                     SEKKKPYKEMMSEDELIEAVKMETAGKPSRPLYTPRLLSMKPRFI"
BASE COUNT         1639 a         2633 c         2636 g         1235 t
ORIGIN      
        1 gtcgagatcg aggacgacga ggggcacgag aagatcgtcc ggatcacccg ggtgcacctc
       61 gaggaggacc cggggcgcct cgtccacgtc gcgggcgcat cgaagtactc gctcgtcgac
      121 tacaaccggt cgggtatacc gctcctcgag atcgtcaccg aaccggatat gcgctccccg
      181 caggaggcgc gccggttcct gaacaagctc aggacgatcc tcgagtacct gggagtcttc
      241 gacggcgagc gggaaggcgc tctccgtgtt gacgcaaaca tatcgatcga gggttcggcg
      301 agggtcgagg tcaagaacat atcctcctac aaaggcgtcg agaaggccct caccttcgag
      361 gcgacccggc agaggaacct gctccggcgc ggccagacag tggcaaggga gacccggcac
      421 ttcatggaag ggcgcgggat caccacatcc gcccgcggca aggaggaggc gcacgactac
      481 cgctacttcc ccgacccgga tctcgggccg atccgcgtcg cagaccgggt cgcggcgatc
      541 gacctccccg aacttcccac cgcgcggaag aaccggttca tggcgcagta cggcatatcg
      601 ctaaaccacg cccggacact gaccggcgac ccgcggcttg ccgacttcta cgagcacgtg
      661 gcgcccgccg atccgatcct cgccgccacc tgggcggcag atatcctcct tggggagctc
      721 aactaccgcg acatgagcat cgccgccgtc ccggccgacc gcatacgaga actcctcgcc
      781 ctcctcaggg cggagacgat caccgatgcg gccggcgtcc aggtcctgcg ggagatgctc
      841 gatgcctgcg ccgcaggccg gccctgcgag aaacccgccg atatcgtgaa gcgcgagggg
      901 cttgccagag ccacggggga cgactttgtc gcgatcgtgc aggcggtcat cgcctcaaac
      961 ccgcaggcag tggaggacta ccgtgccggc aagaagggag ccctgaactt tatcgtgggg
     1021 caggtgatga aagagacccg cgggcgggcg gacccgcgag aactcgggcg aatcgtatcc
     1081 gaatgtatcg agaaaggcgg ggtgtaatcc gcagtgcacc tgatcatagc agaaaaaaac
     1141 atatcagcaa accggatcgc gcagatcctc gccggcagcg agaaggtgag ggcgtcgaag
     1201 gatgggagcg tatcgaccta ctccttcgat acgaagacgg ttgtgggcct caaggggcac
     1261 gtggtggagg tggacttcga gcccggctac acgaactgga gaagcgaggt ccacacaccg
     1321 cgaagcctca tcgacgccgg catcgtcaag aagccgaccg agaagaagat cgtcaacctc
     1381 atccagaaac tctcgaagaa ggcggacctc gtcacgatcg ccaccgacta cgatacggaa
     1441 ggcgaattga tcgggaaaga ggcctacgaa ctcgtccgcg cggtgaactc cacggtcaag
     1501 atcaaccggg cccggttctc ggcaatcacc cctgcagaga tccggaccgc ctttgagaac
     1561 ctgaccgatc tcgacttcgc cctcgcggcc gccggcgaag cccgccagac ggtcgacctg
     1621 atgtgggggg cgtcgctgac ccggttcatc agccttgccg cgcggcgggg cggcaaagac
     1681 atcctctccg tcggccgggt ccagagcccg acgctcgcta tgatcgtcga ccgcgaacgc
     1741 gcgatcgaga acttcgtccc ccagacctac tggatgctct cgctcctgac ggagaaggac
     1801 ggcgtgccgg tggaggcgcg gcataccgcc ggacggttca ccgaccacga tgcggccctt
     1861 gccgccgagg cggggacgaa agagcccctc acggtcaccg acgtcaagga agggcagaag
     1921 accgaccggg cgccgacacc cttcgacacc acgacgttca tcgtcgcggc aagccgcctg
     1981 ggcctctcgg cggcaaatgc catgcggatc gccgaagacc tctacatgaa cgggtacata
     2041 tcctacccga ggaccgacaa cacggtctac ccgaagtccc tgaacctgaa cggcatcctc
     2101 gagacgcttc ggggaggggt cttcgatgcg gacgtcgcct gggtgcagca gcaccggcga
     2161 tcggtcccga cccggggcaa gaaggagagc accgaccacc cgccgatcca cccgacggga
     2221 gcagcgaccc gcgaggccct cggccaggac cggtggaagg tctacgaact cgtcgtccgg
     2281 cggttcctcg cgaccctctc tcccgacgcg acctggacga cgacgaagtg cctcttcgac
     2341 gcctccggcg agccctacac cgcgacgggc gggcggctcc tctccgcggg atggcgccgg
     2401 atctacccct actcggaggc aaaagagaac atactcccgg tatttgccgt gggcgagcgc
     2461 ctgccgatcc ggaaggtgaa tctcgaagag aaacagacgc agccgccggc acgcttctcc
     2521 cagagccggc tcatccaggt gatggaggag ctcggcctcg gcacgaagag cacccggcac
     2581 gaggtgatcg ggaagctcat atcccgccgc tacgtggaag gaaacccgct ccgcccgacc
     2641 ctcgtcggga gggccgtcac ggatgcgctc gacaaccacg cggagacgat caccgagccc
     2701 gagatgacga gaacgcttga agagcatatg cagctcatca agcagcgcga gcgctcccgc
     2761 gacgacgtcg tcgacgaatc cagggagatg cttcataggg tctttgacaa gctcgaggcg
     2821 cacgagaagg agatcggcga tgagatcatg ggccagacgg cggaagagca taccgtcggc
     2881 ccctgccccg cctgcggcca cgagctccgg atccggcatc tcggcgcttc tcagttcatc
     2941 gggtgcaccg gctacccgga gtgccggttc aacatcagcc tgcccggcag cacctggggg
     3001 aaggcgatcc ggaccgacga ggtctgcaag acgcacaaac tcacccacgt tcgccttatc
     3061 cggaaaggat cccccccgtg gacgatcggc tgcccgctct gcagccatat cgcctcgaac
     3121 atcgaggcgc tccgtatgat gccctcgatg accgacagcc tcgtagagcg cctgcacgca
     3181 caccacatct acacggtatc cgagatcgcg ggcatgcagc cccacgacct tgcgggtgcc
     3241 gccggcgtcg gcgcggagga ggccgggcaa ctcatccggg aggccggggg cgcgctcgat
     3301 atactccggc gccgctcgga gctccggaaa ttcatcagga agatcgtccc cccaaagaag
     3361 ggccggagcc acgcgaagat aacaaaaagc ctcgtcgagc agggggtcgg ggatatccgc
     3421 gcgctctcgc ggtctgactc tgcggtcctg aagaaagccg gtatcggcga gacgccggcg
     3481 gcagagctcc tcgcggcggc ccgcggcctt tgcaacgagc gcaccctcag ggaagccggc
     3541 attcccgcgg tcagcctgaa gaagtaccag gcagggggca tcgcaagccc cgacgacctc
     3601 tgctacctcc cggtccccta cctcgcaaac aaaaccggca ttaatccgga gaccgtacat
     3661 aaacatgctg atatggtctg caggcacctc gggcgccccg ccccggaaaa ggtcacgaag
     3721 accgcgcttg agcgcgggcg aaaagagctc ctggcggtcc ccggcatagg ggaagccacc
     3781 gtggagaaac tctaccttgc gggcatatac gacgccgcga cgctccgcga ggccgacccc
     3841 ggggaagtct cctccatcac cggcatcgcg aagacgagga tccacgacta catcagccac
     3901 ctgaagtgac accatgcacg cgaactggaa gacctgggca catgtgacga agctggaccc
     3961 cgacaaacgc cttccaccgg gcgccgccga ggagatcgca acgagcggga ccgatgccct
     4021 gatgctctcg ggcaccctga acgtgacgca ggagaacctc aacgagcttc tggaccaggt
     4081 ctccccctac gggctgccgc tggtcgtgga gccggcgagc cccgactgcg ccatattcga
     4141 cgggcggatc gatcatctct tcgtgccgag cgtggtgaac gcaaacgacg tccgctggat
     4201 cgtcgggaaa cactacacct ggctccgcca cgcaaacagc atcgactggg atatggtggt
     4261 gcccgaggcc tacatcgtcc tcaatccgaa ctccgccgtc gggcgggtga cgggggcgga
     4321 ctgcgccctc tcgggcaggg atgtggccgc attcgtagag gtggcggacc gttacttccg
     4381 gttcccgatc gtctacatcg agtacagcgg aagatacgga gacccgtcga tcgtgcaggc
     4441 ggcatccgat gcggtcgagc acgccgtcct ctactacggg ggcggcatcc gttcggcgga
     4501 gcaggcggcg gagatgggcc ggatcgcgga tacgatcgtc gtcggcaacg ccgtctacga
     4561 tgaggggatc gatgtgctcc gggcgacggt ccgggcggta cagtgaggca tgcctgaggt
     4621 ctcgatcgtc ctggtcgaac ccctctacga ggggaacgtc gggtttaccg cccgggtgat
     4681 gaagaacttc gggttcaccc ggctggtgct ggtcaacccc tgcgcgctcg gcgacgacgc
     4741 catcatgcgc gccgcccatg cccgcgacgt cctcgacggc gctcgccgga tgacgatcga
     4801 cgatgtctac cgggagttcg acttcgtcgt cgcgacgacc ggcgaggtga gtaagtcggt
     4861 ctgcacgccg atgcggatgc cctactacgc gccggcggaa gtccgggagg ccgtcgccga
     4921 tatcgacggg acggtcgcga tcctcttcgg ccgggagaac tggggcctcg cgaacaccga
     4981 acttgcgcgg gcaaacttca tctgcaccat cccgacatcg gaggcatacc ctatcctgaa
     5041 cctctctcac gcggtgggca tcctctgcta cgaactggcc aacctgcccc gcgggaccta
     5101 cctccttgcg gggcaggtcg agatggagtc gctctaccgg cacatcgaga cgttcctcga
     5161 ccggatcgac caccctgact tcaaacggga gaacacgatg gtcctcctcc ggcgcgtgct
     5221 cggccggacg aaactgaccg tccgcgaggt gagcacgatg cacgggctta tgcgccggac
     5281 ggagtggcat ctcgaaaaca aagaataggt tcgaggagaa gagctaaagg aatgattctt
     5341 gtcgactggc agatagagga ccgtatcagg cgcgggtata tacgggtcga cccctacgac
     5401 cccgccctga tccagcccaa ctccctggat atcaggctcg gttcccactt cgtctggtac
     5461 gtgccggggg atattgtgat cgatccctac gagagggaga gcgtctgcgc agatgtcgag
     5521 gaggtagtgg ccgactcgat cgtcctcgcg ccggggcagt tcctccttgc aaagaccctg
     5581 gagacgatcg agctccccga cgacgtcgtg gcgagcatcg aggggaagag cagcatcgcc
     5641 cgcctcggta tcgagctcca ccagacgggg ggctggatcg acgccggatt ccaggggacg
     5701 ataacgcttg agatgtgcaa cgtgaactcc cggccggtcc gggtctacgc cgggatgcct
     5761 atcgggcagc tcgtcttcta ccggacggat cgcgccgcgc gcccctacaa catgaagaag
     5821 gacgcgaagt acatgggcca gcagcaggcg accctctccc gctaccacga gaatgcgcgc
     5881 catgcgtgat gcatactgcc ggtagtccgg aaaagacccc gcgcattcca gctccggcga
     5941 gcagggtgcc gggtgttccg cccgggagag cgttatgcac tatatagcag gaaaacagat
     6001 attcaacggt tcaactgggg ataccaatgc gacttcttct gatccactct gaccatatcg
     6061 aatatgaggc acggaagaag acgaaggtcg ccgaagagga tgcggtccca aaggacgctc
     6121 tcgatgaggc gctcgccgtc ttctgcgcgg ttgagtctgt cgacgaggag aatatcgggg
     6181 atgccgtcag gcaggcggcg gacgagatcg tcactaccgc ccggcagctc ggcacgacca
     6241 atatcatgat ctacccctac gcccacctct cctccgatct tgcatcgccg gaagcggcgg
     6301 tgagcgctct ccggggcatc gaggaagccc tctccgggga aagcgacttc gtcgtgaagc
     6361 gggcgccgtt cggctggtac aaggccttct cactctcctg caagggccac ccgctctcgg
     6421 agctctcccg gacgatcgtg cccggggagg gaacggccgc gccgaagaag gagatcgagc
     6481 acgagttctt cgtcttcacc cccgaagggg agcgcaagga tgcggccgcc tacgtggatg
     6541 agaagacgcc cttcgccgcc cttatccgga aagaactcgg gtatccgggg ccggaagggg
     6601 tcgaaccggt tcacgtcgac ctgatgcggg caaaagagct cgtggagtac gaaccgcgct
     6661 ccgacgtcgg gcaccaccgc tggatgcccc ggggcaagct gatccgcgac cttctcaccg
     6721 actacgtcct tacgcaggtg ctcgagtacg gcggtatgcc ggtggagacc ccggtgatgt
     6781 acgatctcgg cgaccaggcg atcgcggagc acgccgccaa gttcggggag cggcagtatc
     6841 ggttcaagag cggcaaccgc gacatgatgc tccggttcgc ggcgtgcttc ggcatgttct
     6901 cgatcatgca cgacatgcac atatcgccga acaccctccc gatgaagctc tacgagctct
     6961 cgacctactc gttccggcac gagcagaagg gcgaggtgat cgggttgaag cgcctccgcg
     7021 ccttcacgat gcccgatatg cacaccctct gccgggacgt tgacgatgcg ctcgcgtcgt
     7081 tcgaggagca gcttgccatg ggctggaaga gcggcgagga tctcgagacc ccgctcgtag
     7141 gggtcttccg gtgcacccgc gacttcttcg accagtacga actctgggtg aagcagcttg
     7201 tcgcgaagtc aggcgtcccg atgctgatcg aggtcctctc ggaccggacg cactactgga
     7261 tagcaaaggt cgacctcgcg gcgatcgatg cacagggaag accgatcgag aacccgacgg
     7321 tccagatcga cgtcgagagc gcggaccggt tcgacatcaa gtactacacc ccggagggtg
     7381 tggaggtcca ccccccgatc ctccactgtt cgccgaccgg ctcgatcgag cgggtcatct
     7441 gcgcgctgct cgagaacacc gcgcagatgg aggtcccctc gctcccgacc tggctcgccc
     7501 cgacccaggt caggctggtc ccggtggcgg ggcggcacgt ctgctttgcg gaggagatcg
     7561 gtgcacgcct gaacgccgcg ggcattcggg cggatgtcga cgaccgcgac gagagcgtga
     7621 acaagaagat ccgcgaggcc ggcatggact gggcgcccta cgtcgcggtg ataggcgacc
     7681 aggaggccga gaccggccgg ctcacggtca ccatccggaa gctctcggag aagaagaagc
     7741 cgtataagga gatgatgagc gaggacgagc tcatcgaggc ggtgaagatg gagaccgccg
     7801 gaaagccttc ccgcccgctc tacaccccgc ggctcctctc gatgaagccc cggttcatat
     7861 aacctctttt ttcgcgaacc tctttgagat ggagtgagct gctgaagcct gagcgcagcc
     7921 gccgtcgggc atggcccctt cgggcggcca gacccggcga tggcggggcg gggcaaaacg
     7981 gtcgagcggg gagaacgcga tgcaggggcc gtggcggcta tcgctccggg cactgccctt
     8041 accccgggat aggcggggcg ggggggccgg cagagagcgt atgcttaacc ggcaggaggc
     8101 gcgggcaagg gatgccggcg agaagtaaga tggttggagt gtg
//
LOCUS       DAOSEE010000093         8137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_3278_length_8137_cov_214.955828, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000093 DAOSEE010000000
VERSION     DAOSEE010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8137
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3278_length_8137_cov_214.955828"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="PLI76_07735"
     CDS             <1..746
                     /locus_tag="PLI76_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOI61961.1"
                     /translation="KRYLSVDRGVVRAVNGVSFNVGEQEIFGIIGKSGAGKTTLSRII
                     SGILEPTSGEMNVRIGDDWVDMTKPGIEHRGRAKGYIGLLHQEYDLYPHRTVLDNLTD
                     AIGLEFPKELAMRKAIITLGMAGFTPEKSREILDRYPGQLSEGEKHRVALAQVLIREP
                     LLVVLDEPTGTMDPITKIDVKHSILHAREEMDETFIVVSHDMEFVRDICDRVALMRGG
                     KIIRLGPTEEVLAHLTDEERKVMGTGGAP"
     gene            754..2283
                     /locus_tag="PLI76_07740"
     CDS             754..2283
                     /locus_tag="PLI76_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012108012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 3 protein"
                     /protein_id="HOI61962.1"
                     /translation="MMEIYLDRDRIEVPEGARLGDILTGWDARCSVAVIRPALEEDAT
                     ESQEVRFATSAGDMVVEMADPALVSRLFAPGLAERLRLHWQDRYAAAFGPFETGIRPA
                     RAPGRYERGDVILGCGGYDPARSYLIFARMRHTADYGAPADGGVIGRVVTGRGLLDRI
                     AEGDRVVEIERILRRADRSHAIVTRDPGLPLEDGMQVVSSIEAEVQGFTGGEIDTRAA
                     RSVEHFLLSVQSGRFRVDRSSSTHITDTHLVPTKVPPEFIGPRLEGSITVRTAGKSSG
                     ATYIYTQGVSASPAHTVVGRVVHGLELVRFSSDGDLLAIRVEPEQFDLVGLTLAEAEA
                     VAERRGIALTADAAGGDRMVVGQTPATTLEVLSAGAARVATEAAENVVSITLDEAAAP
                     RSVTIFREVTGLKHHAVGKMPLVFRFEDVSLFKPKIGKGVGIIPENTPTGEVPAFTLA
                     MTNDSRRGAGMVGIRETPNAEFGPTAEPLTGTNVIGKVLDAGSLAKMREGTTVYVREV
                     K"
     gene            2284..2739
                     /locus_tag="PLI76_07745"
     CDS             2284..2739
                     /locus_tag="PLI76_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 6 protein"
                     /protein_id="HOI61963.1"
                     /translation="MAEYIPGYVGTVTKYVFVESPVMTPGELALRAYEASEGVLIKET
                     CFGLQVTGEPEAVDRLIARIRTFDPMHIFVKDRGFPPGDPRRCRANLGGARPGYLGHE
                     REFRILRYITRGLEEVERRGEEAETPEPAPEKRSGITIRRLQELIDEES"
     gene            2745..3197
                     /locus_tag="PLI76_07750"
     CDS             2745..3197
                     /locus_tag="PLI76_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 5 protein"
                     /protein_id="HOI61964.1"
                     /translation="MAKVFIYPATSLILSDLVARFGHKPLGAALGIRERIQTAGVDSP
                     PLQITPEEPKRGLKYAAVEVPSGVRGRMAIYGPLIEEAEAAVIVTDADFAFGCMGCAR
                     TDELILFSLRQSGIPILELKYPTTDDEGVRFVAAIKKFLAGLPNGGAA"
     gene            3194..4432
                     /locus_tag="PLI76_07755"
     CDS             3194..4432
                     /locus_tag="PLI76_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 15 protein"
                     /protein_id="HOI61965.1"
                     /translation="MSQKVRIAQLSCGPEYSGVQKEIYDAAGSVGAEVFFPDIALADV
                     ERAVDRFGLDVRSADLKLMIARGMALVDGKVDADGVFIGTCFRCAEAAIVRNELRRYI
                     PEHSRLPVVSYSFTERTTAGTLLTRMEALTTIARRRALLAREEQTGITMGVDSGSSTT
                     KAVVMKDNEIVGTGWHPTTEVIASADSVVADALAMAGLVPDDIQAIGTTGYGRFLIGK
                     HFGAELVQEELTVNSKGAVYLAGHQKGSATVIDIGGMDNKAISVIDGIPGTFTMGGIC
                     AGASGRFLEMTAKRLGVDITELGPLAMKGMGKNVPMNSYCIVFGTQSLVNALAAGSSR
                     EDVAAAACHSVAEQVFEQQLQEVDIKEPVIMVGGTSLIEGLVYAMGQLLQTEVVVPPY
                     SQYIGAVGSALLASGFVKGE"
     gene            4433..5017
                     /locus_tag="PLI76_07760"
     CDS             4433..5017
                     /locus_tag="PLI76_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 17 protein"
                     /protein_id="HOI61966.1"
                     /translation="MPPLNYFVVESPDPVGREAYERITGDVLQDLDLIKVIDRLHIYV
                     DPKVPIFIAAGVLRHMPRSIRVGDFANVNPGEKLIVLDIGDETYLAPLLRELWAHYGK
                     ENVDQPDRFTIVLPAGVVGEEELEQMVVADPSETLHKDIIYALQYIAPEGFKVRRQYI
                     KDEKFYYVASEDTLPGDIVETMVVPIFAKMGVTL"
     gene            5014..5955
                     /locus_tag="PLI76_07765"
     CDS             5014..5955
                     /locus_tag="PLI76_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 7 protein"
                     /protein_id="HOI61967.1"
                     /translation="MTTLVPVTYKGGVYRHDEIMDLIDDLGGYIVQKHVMAQDVVLQS
                     FVPRDDIDLIRTVAKPLAGDVIEAPLVGTEIAVVSPSLEIHHLPHTACDIAEYLRRSG
                     AKTNMIGLARGFGKRIANLNDEERDVINEHDLAVYVLGNFEECIEHKFPALRRGIQVP
                     IVVTGAPDRETLARIVDPPVEGYVGGVGRIMHRFKRPEELAKLDELVDEVSRALDARR
                     DALARDPLSVFPPRLMAIVEEQIPEVTMLTHPTPVTAQMEGLRVKLPYDLYADDVRSL
                     DVTGGVTVGDIADVLPSRMRNYILIRIKPFSETRILV"
     gene            5958..6404
                     /locus_tag="PLI76_07770"
     CDS             5958..6404
                     /locus_tag="PLI76_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOI61968.1"
                     /translation="MDFERILARVAERGSDAIARELLREIENEYGRVPLIFERMAERP
                     EILISHLLYKGAVVETTRLDPKTIELISLAVGAALKCTHCVEYHMQAAKAKGATRDEI
                     LEAILIAGMLANSAVLADAYRVVNGSIPCASCDINGTGLDKTCLDE"
     gene            6417..>8137
                     /gene="asnB"
                     /locus_tag="PLI76_07775"
     CDS             6417..>8137
                     /gene="asnB"
                     /locus_tag="PLI76_07775"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOI61969.1"
                     /translation="MCGIAGMVAPDGEAADPTLVRAMAERLAHRGPDGEGEFFSGPVG
                     LAHRRLAIVGLSDEGRQPMANGDGSLQIVFDGEIYNFPEVREALVAAGHTFATATDTE
                     VILHAYEEWGHDCLSRFNGMWAFALWDGRRRELFCARDRLGVKPFYYTADAGSFLFAS
                     EIKALLVHAGRRPNDRMLLTFLAWGVADHTPETMYEGIVQLPPAHFVVVSEAGAGEPE
                     RYWDIAVNGAPRGADDEEAAQGVRSLLADAVRLRLQSDAPVGICLSGGIDSSTVTALA
                     NVLLQSGHPKSVGDRQKTFSVSFEDPRFDESRHIDTVVAATGVANRRTTPDTEGLWED
                     IGRLLYMQDEPFASLSIYAQYCVMRLASSEVKVVLDGQGADEQLAGYIAYQMPYIRGL
                     LRRGEVLAALREVAGSVRRHTSFFSWAARQFAVRSERRGLLKGDAPEVLRYAGSLDEV
                     LAREITASNLPLLLHWGDRNAMAFSIEARVPFLDYRLVEYLAGLPLDQKIRSGVTKYV
                     LRRAIEGLVPDAIRCRMDKMGFVTPEEAWMKDELRPHILALFSSPEFARRPYWDAERV
                     LRNYREFL"
BASE COUNT         1460 a         2582 c         2768 g         1327 t
ORIGIN      
        1 tcaagcgcta cctctcggtc gaccggggcg tggtcagggc ggtcaacggc gtctccttca
       61 acgtcggcga gcaggagatc ttcggcatca tcggcaagag cggtgcgggc aagacgaccc
      121 tctcgcggat catatccggg atccttgaac ccacgagcgg cgagatgaac gtccggatag
      181 gcgacgactg ggtcgatatg accaaacccg gcatcgaaca ccggggacgg gcgaaaggct
      241 acatcgggct tctccaccag gagtacgacc tctacccgca cagaacggtg ctcgacaacc
      301 tcaccgacgc cataggcctc gagttcccaa aagaacttgc catgcgaaag gcgatcatca
      361 ccctcggtat ggccgggttc acccccgaga agagcagaga gatcctggac cgttacccgg
      421 ggcagctctc ggaaggagag aaacaccggg tggcgctcgc ccaggttctc attcgcgagc
      481 ccctgctcgt cgtcctcgac gagccgaccg gcacgatgga ccccatcacg aagatcgacg
      541 tgaaacactc tatcctccac gcccgcgagg agatggacga gacgttcatc gtggtctcgc
      601 acgacatgga gttcgtgcgg gacatatgcg accgggtggc gctgatgcgg ggcggcaaga
      661 tcatcaggct ggggccgacg gaagaggtgc tcgcgcacct caccgacgag gaacgaaagg
      721 tgatggggac cggcggcgcc ccctgaggca cgaatgatgg agatctacct ggacagagat
      781 cgtatcgagg ttccggaggg cgcccggctc ggggatatcc tcaccggatg ggacgcccgg
      841 tgcagcgtcg ccgtcatccg cccggcgctc gaggaggacg cgaccgagtc gcaggaggtc
      901 aggttcgcca cgtcggcggg ggatatggtc gtcgagatgg cggatccggc cctggtttca
      961 aggctctttg cgccggggct tgccgaacgg ctccgcctgc actggcagga ccgctacgcc
     1021 gccgccttcg gaccgtttga gaccggcatc cgccccgcgc gggcgccggg gcggtacgag
     1081 cggggcgacg tcatactcgg gtgcggcggc tatgaccctg cccggtccta cctgatattc
     1141 gcacgcatgc ggcataccgc cgactacggc gccccggccg acggcggcgt catcggcagg
     1201 gtcgtcaccg gccgggggct gctcgaccgc atcgccgaag gcgaccgggt cgtcgagatc
     1261 gaacggatac tcaggcgcgc agatcggtcg cacgcgatcg tcacccgtga ccccgggctg
     1321 ccgctcgagg acgggatgca ggtcgtctcc tctatcgagg cggaggtgca ggggtttacg
     1381 ggaggcgaga tcgatacccg ggccgcccgg agcgtcgaac acttcctgct ctcggtgcag
     1441 agcggccggt tccgggtgga ccgttcaagc agcacccata tcaccgacac ccacctggtc
     1501 ccgacgaagg tcccgccgga gttcatcgga ccccggctcg aggggtccat cacggtcagg
     1561 accgccggga aatcctcggg agcgacctac atctacacgc agggcgtatc ggcaagcccg
     1621 gcccacaccg tcgtcgggcg ggtggtccac ggccttgaac tcgtgcggtt ttccagcgac
     1681 ggcgacctcc ttgccatccg cgtcgagccc gaacagttcg acctggtcgg cctgaccctt
     1741 gcggaggcgg aggcggtcgc cgagcggcgg gggatcgcgc tcaccgccga tgcggccggc
     1801 ggagaccgga tggtggtcgg ccagacgccg gcaacgacgc ttgaggtgct ttctgccggt
     1861 gcggcccggg tcgcaacgga ggcggccgag aacgtcgtca gcatcaccct cgacgaggcg
     1921 gccgcgcccc ggtcggtcac gatctttcgc gaggtgaccg ggctcaagca ccacgccgtc
     1981 gggaagatgc cgctcgtctt ccggttcgag gacgtctccc tcttcaagcc gaagatcggg
     2041 aagggcgtcg ggatcatacc ggagaacacc cccaccgggg aggtcccggc gttcaccctt
     2101 gcgatgacga acgattcgcg gcggggggcc ggcatggtcg gcatacggga gacgccgaac
     2161 gccgagttcg gcccgaccgc cgagcccctc accggcacga acgtcatcgg gaaggtgctc
     2221 gatgcgggga gccttgctaa aatgcgcgag ggaacgacgg tctacgtgag agaggtgaag
     2281 tgagtggctg agtatatacc ggggtacgtc ggcacggtca cgaagtacgt cttcgtcgag
     2341 tcgccggtga tgaccccggg cgagcttgcc ctcagggcat acgaggcgtc cgagggcgtc
     2401 ctcatcaagg agacctgttt tggcctgcag gtgacgggcg agccggaggc cgtcgaccgg
     2461 ctcatagccc ggatacggac gtttgacccc atgcacatat tcgtcaaaga ccgcgggttc
     2521 ccgcccggcg acccgcggcg gtgccgggca aacctgggcg gggcgcggcc gggctacctc
     2581 ggccacgagc gcgagttccg catcctgcgc tacatcaccc ggggtctcga agaggtcgag
     2641 cggcggggag aagaggcgga gacgccggag ccggcccccg agaagagatc cggaataact
     2701 atcaggcgac tacaagaact catagacgag gagtcctgaa gagaatggca aaggtgttca
     2761 tatatcctgc aacgagcctt atcctctccg acctcgtggc ccggttcggc cacaaaccgc
     2821 tcggtgcagc ccttggtatc cgggaacgga tacagaccgc cggggttgac tccccgcccc
     2881 tgcagatcac gcccgaagaa ccgaaacgcg gcctgaagta cgcggccgtc gaggtgccgt
     2941 ccggtgtccg gggacggatg gcgatatacg gcccgctcat cgaggaggcg gaggccgcgg
     3001 tcatcgtcac cgacgcggac ttcgcgttcg ggtgcatggg gtgcgccagg accgacgagc
     3061 tgatcctctt ttccctgcgc cagagcggca taccgatcct cgaactgaag tatcccacga
     3121 ccgacgacga aggcgtccgg ttcgttgccg ccatcaagaa gttcctcgcc ggcctcccga
     3181 acgggggtgc agcatgagcc agaaggtgcg gatcgcccag ctctcctgcg ggcccgagta
     3241 cagcggcgtc cagaaagaga tatacgacgc tgcaggatcg gtgggcgcgg aggtcttctt
     3301 ccccgacatc gctcttgccg acgtcgagcg ggcggtcgac cggttcggcc tcgatgtccg
     3361 gagcgccgac ttgaaactga tgatagcccg gggcatggcg ctcgtggacg ggaaggtgga
     3421 cgccgacggc gtcttcatag gcacctgctt ccggtgcgcc gaggcggcta tcgtccgaaa
     3481 cgagctccgc cgctacatac ccgaacactc gcggctcccg gtggtgagtt actccttcac
     3541 cgagcggacg accgcaggca ccctcctcac ccggatggag gccctgacca ccatcgcccg
     3601 ccgccgggcg ctccttgccc gcgaggagca gaccggtatc acgatgggcg tcgactcggg
     3661 gtcgagcacg acgaaggctg tcgtgatgaa agataacgag atcgtcggca ccgggtggca
     3721 cccgacgacc gaggtgatcg cgagcgccga tagcgtggtt gccgacgccc ttgcaatggc
     3781 cgggcttgtc cccgacgata tccaggcgat agggacgacc gggtacggcc ggttccttat
     3841 cgggaaacac ttcggcgccg aactcgtcca ggaagagctg acggtgaact caaagggcgc
     3901 cgtctacctt gccggccacc agaaggggtc tgcgaccgtc atcgatatcg gcggcatgga
     3961 caacaaggcg atcagcgtca tcgacggcat acccgggacg ttcacgatgg gcggcatatg
     4021 tgccggcgca agcggcaggt tcctcgagat gaccgcaaaa cgtctcggcg tggacatcac
     4081 cgaactcggc cccctcgcca tgaagggcat gggcaaaaac gtcccgatga acagttactg
     4141 catcgtcttt gggacgcaga gcctggtgaa cgcgcttgcc gccgggtcgt cgcgggagga
     4201 tgtggcggct gcggcctgcc acagcgtcgc cgaacaggtc tttgaacagc agctccagga
     4261 ggtcgacatc aaggagccgg tgatcatggt gggcggcacc tccctgatcg aggggctggt
     4321 ctacgcgatg ggccagctcc tccagacgga ggtcgtcgtc ccgccctact cgcagtacat
     4381 cggcgcggtc gggtcggccc tgctggcatc cggattcgtc aaaggagagt agatgccccc
     4441 gttgaactac ttcgtcgtcg agtcgcccga tccggtcggg agagaggcct acgagcggat
     4501 aaccggcgac gtcctccagg acctcgatct catcaaggtg atcgaccgcc tccacatata
     4561 cgtcgacccg aaggtgccga tcttcatcgc ggccggggtg ctccgccaca tgccgcggtc
     4621 catccgggtc ggcgactttg ccaacgtcaa ccccggcgag aagctgatcg tcctcgatat
     4681 cggtgacgag acctaccttg cgccgctgct cagagagctc tgggcccact acgggaaaga
     4741 gaacgtcgac cagcccgacc ggttcaccat cgtcctcccg gcgggggtgg tcggggagga
     4801 ggagctcgag cagatggtcg tcgccgaccc gagcgagacg ctccacaaag acatcatcta
     4861 cgccctccag tacatcgccc ccgaagggtt caaggtccgc cgccagtaca tcaaggatga
     4921 gaagttttac tatgtggcaa gcgaggatac cctcccgggc gacatcgtgg agacgatggt
     4981 tgtaccgata tttgcaaaga tgggggtgac gctgtgacga cactggtgcc ggtgacctac
     5041 aagggcggtg tctaccggca cgacgagatc atggacctga tcgacgacct cggcggctac
     5101 atcgtgcaga agcacgtcat ggcccaggac gtcgtgctcc agtcgttcgt gccgcgagac
     5161 gacatcgacc tgatacggac ggttgcaaaa ccgcttgccg gcgatgtgat cgaggcgcct
     5221 ctcgtgggga cggagatcgc cgtcgtgagc ccgagccttg agatccacca tctcccccac
     5281 accgcctgcg atatagccga atacctccgg cgctcgggcg cgaagacgaa catgatcggg
     5341 cttgcccggg gatttgggaa gcggatagcg aacttaaacg acgaggagcg ggacgtcatc
     5401 aacgagcacg accttgccgt ctacgtgctc gggaacttcg aggagtgcat cgagcataag
     5461 ttcccggcgc tccggcgggg gatccaggtg ccgatcgtcg tgaccggagc accggaccgc
     5521 gagaccctgg cccggatcgt cgatccgccc gtcgaaggct acgtcggcgg tgtggggagg
     5581 atcatgcacc ggttcaagcg ccccgaggag ctcgcgaaac tcgacgagct ggtggacgag
     5641 gtctcccgcg ccctggacgc ccggcgggac gcccttgcac gggacccgct ctccgtcttc
     5701 ccgccccgcc tgatggcgat cgttgaagag cagatccccg aggtcaccat gctgacgcac
     5761 ccgacgccgg tgaccgccca gatggagggg ctccgggtga agctccccta cgacctctac
     5821 gccgacgacg tgcggtcgct cgatgtcacc ggcggggtca ccgtcgggga tatcgccgac
     5881 gttctgccgt cgcggatgcg caattacata ttgatacgga tcaaaccctt ctcggagacc
     5941 aggatattgg tgtagcgatg gattttgaga ggatactggc gagggttgca gagcggggat
     6001 cggatgctat cgcacgcgag ctcctccggg agatcgagaa cgagtacggc agggtccccc
     6061 ttatattcga gcgtatggcg gagcggcccg agatcctgat atcgcacctg ctctacaagg
     6121 gcgccgtggt cgagaccacc cggcttgacc cgaagacgat cgagctgatc agccttgcgg
     6181 tcggcgccgc gctcaagtgc acccattgcg tcgagtatca catgcaggcg gctaaggcaa
     6241 agggcgcgac ccgggacgag atcctggaag cgatcctgat cgccggcatg ctcgcgaact
     6301 ccgcggtcct tgcggatgcc taccgggtgg tgaacggatc gattccctgt gcgtcctgcg
     6361 acatcaacgg caccggcctg gataagacct gcctggacga gtgacggggg cccgacatgt
     6421 gcgggatagc aggaatggtc gccccggacg gggaagcggc ggatccgacg ctcgtccggg
     6481 ccatggcgga acggctcgcc caccgcggcc ctgacggcga aggggagttc ttctccggcc
     6541 cggtggggct ggcacaccgg cgccttgcga tcgtcggtct ctccgatgag gggcggcagc
     6601 cgatggcaaa cggggacggg tcgctccaga tcgtcttcga cggcgagatc tataattttc
     6661 ccgaggtccg cgaggccctc gtcgccgccg gccacacctt tgccacagcg accgatacgg
     6721 aggtgatcct gcacgcctac gaggagtggg ggcatgactg tctctcccgg ttcaacggca
     6781 tgtgggcgtt cgccctctgg gatggccggc gccgcgagct cttctgcgcc cgtgaccgcc
     6841 tgggcgtcaa gccgttctac tacacggcgg atgccggatc gtttctcttt gcctcggaga
     6901 tcaaggcgct cctcgtgcat gccggccgga gaccaaacga ccgcatgctc cttacgttcc
     6961 tcgcctgggg ggtcgcggac catacgccgg agacgatgta cgaggggatc gttcagctcc
     7021 cgccggccca cttcgtcgtg gtgtcggagg cgggcgccgg ggagccggag cgctactggg
     7081 atatcgcggt gaacggtgcc ccgcgggggg ccgatgacga ggaggccgcg cagggggttc
     7141 gatcgctcct cgccgacgcg gtccggctcc ggctccaaag cgacgccccg gtcgggatct
     7201 gtctttcggg gggtatcgat tcgtcgacgg tcaccgccct ggccaacgtc ctcctgcaga
     7261 gcgggcatcc gaagagcgtc ggcgaccggc agaagacgtt ctcggtcagt ttcgaggatc
     7321 cccggttcga cgagagccgg catatcgata cggtggttgc ggcgacgggg gtcgcgaacc
     7381 gccggaccac ccccgacacg gaggggctct gggaggatat cgggaggctc ctttatatgc
     7441 aggacgagcc gtttgcgtcg ctctcgatat acgcccagta ctgtgttatg cggctggcaa
     7501 gtagcgaggt gaaggtcgtc ctcgacgggc agggggccga cgagcagctt gccgggtata
     7561 tcgcctacca gatgccgtat atccgcgggc tcctccggcg gggggaggtg cttgcggctc
     7621 tccgggaggt tgccgggagc gtgcggcgtc atacgtcgtt cttctcgtgg gcggcccgcc
     7681 agttcgccgt ccggtcggag cggcgggggc ttctcaaggg ggatgcgccg gaggtcctcc
     7741 ggtacgcggg atcgcttgac gaggtgcttg cccgggagat aacggcctcg aaccttccgc
     7801 tcctgctcca ctggggggac cggaacgcta tggcgttctc gatcgaggcg cgggtgccgt
     7861 tcctcgacta ccggctggtg gagtacctcg caggcctccc cctcgaccag aagatccgct
     7921 ccggcgtcac gaagtacgtc ctccggcggg cgatcgaggg gctggtgccc gacgctatcc
     7981 ggtgccggat ggacaagatg gggttcgtca cccccgagga ggcctggatg aaggatgaac
     8041 tccggcccca tattcttgcc ctcttctcgt cgccggagtt cgcgaggcga ccctactggg
     8101 acgcggagcg ggtgctccgg aactaccggg agttcct
//
LOCUS       DAOSEE010000094         8108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_3288_length_8108_cov_250.834844, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000094 DAOSEE010000000
VERSION     DAOSEE010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8108
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3288_length_8108_cov_250.834844"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PLI76_07780"
     CDS             complement(<1..488)
                     /locus_tag="PLI76_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOI61970.1"
                     /translation="MLPGEVYPPAEDSSLLLRAALREVGETDRVLEVGTGSGYVAAGL
                     SGRAAAVVVTDINPHAAACARARGLAAVRTDLFAGLSGPFDLILFNPPYLPTAPEERI
                     DDWLEYALDGGPTGRVVIERFIADAGRVLAPFGRVLLLVSSLTGLDEVRDLFARAGFV
                     SFV"
     gene            complement(491..1264)
                     /gene="rsmA"
                     /locus_tag="PLI76_07785"
     CDS             complement(491..1264)
                     /gene="rsmA"
                     /locus_tag="PLI76_07785"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOI61971.1"
                     /translation="MSAPRDQHFLIDRRAVERIAGFADVSGRRVLEIGPGEGILTRAL
                     LDRGANVVAVEIDPALVEELDLAFADAIDEGRLRIVSGDARKVDIPPFDIVVANLPYS
                     ASSKITFRLLDIGFETAILMYQKEFAQRMIARPGTPDVGRLSVMVQTYASVKPLLELS
                     PSAFRPQPQVRSWVVRVTPHEPPYPIADKRVYADVVRVLFSHRRKTVRKGLRSGKEFF
                     PLDVIERAIAALPDDLLQRRPENLTLCEFALVANTLAGC"
     gene            complement(1261..1824)
                     /locus_tag="PLI76_07790"
     CDS             complement(1261..1824)
                     /locus_tag="PLI76_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF655 domain-containing protein"
                     /protein_id="HOI61972.1"
                     /translation="MKAERKEENAVVIDVLPMGYMSDQRPVYKREPVVLAVGVDQFKL
                     LELVPKAGADIQIHDRVYIGDAERDKIERVKRRIGYEDLTATAKLELPFVVEQIVHEN
                     EQRFVDFFNKSLPITPKFHMLHLLPGIGKKLMWEVIEQREKKPFESFADISGRIKSLP
                     HPARMIVSRVLREIEDPNEKYHVFTLK"
     gene            complement(1842..2192)
                     /locus_tag="PLI76_07795"
     CDS             complement(1842..2192)
                     /locus_tag="PLI76_07795"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase Rpb4 family protein"
                     /protein_id="HOI61973.1"
                     /translation="MKVKRIVSEERMLLPELRDTLLAVEATRLGSGEEMSYELRKSIE
                     HANHLSKTSSDKARDLVGELMQFEKMKEEIAYRIANLMPRTRDELRAIYAKERFNLTA
                     EELDRILELVVTHF"
     gene            complement(2214..2507)
                     /locus_tag="PLI76_07800"
     CDS             complement(2214..2507)
                     /locus_tag="PLI76_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21e"
                     /protein_id="HOI61974.1"
                     /translation="MAHHNGPRKKTRYKFKKDLRRRGIPPVTSVIQDFEVGQKVHVVV
                     EPSVQKGMPHRRFHGKTGTVIGQRGRAWVLEVRDGDTMKQVIARPQHLKAQKV"
     gene            complement(2511..3749)
                     /locus_tag="PLI76_07805"
     CDS             complement(2511..3749)
                     /locus_tag="PLI76_07805"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0031119 - tRNA pseudouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(54/55) synthase Pus10"
                     /protein_id="HOI61975.1"
                     /translation="MDIIETVGAILEYGETCNHCLGRFFGKRSFSLTNEERGRALRIT
                     REIAVNEPHSEPESESCWICGGELSRTDAWAAKVVEAVQGIEFATFLIGTRVPPLIAE
                     SEEMVWSDLALRDPEPLKAEMNREVGKAVSALIGKTADLKRPDIVVILDIAEGTVEVQ
                     VNPVFFVGRYLKYERGIPQTHWDCRACKGAGCEKCDFTGKQYADSVEELIGRPVIEAF
                     DAENAVLHGAGREDIDARMLGSGRPFVMEVEAPRRRSVDLAALEEDINEKAGGRVAVH
                     LAGWADRKTVQSLKSDKAHKKYRILVEIDGPVTQDEFRAALDQLKGVTIRQRTPLRVA
                     HRRADKVRERDVLDIRCTGAQDDRYWVEVVGEAGLYIKELISGDNDRTQPSLARILGR
                     TARVVSLDVVLVETANEFGE"
     gene            complement(3737..4318)
                     /gene="trmY"
                     /locus_tag="PLI76_07810"
     CDS             complement(3737..4318)
                     /gene="trmY"
                     /locus_tag="PLI76_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (pseudouridine(54)-N(1))-methyltransferase
                     TrmY"
                     /protein_id="HOI61976.1"
                     /translation="MKRFAVVGHRAATSGTFSLNDLAGSAGRMDVLCRCINASFFLSH
                     DLRRDVECYLILCGEPGPEKTVLFSGSALRYLSPDERSSAALVKKALALPCGDEFRES
                     TPGVYVRRGGFTRLLAEVPFAVLDEAGEDIRAAPALPEAYLLSDHLNFTPEEEDLIAA
                     YPRYSVGPRSLHADHTITVLLNEMDRRESGWIS"
     gene            complement(4315..5646)
                     /locus_tag="PLI76_07815"
     CDS             complement(4315..5646)
                     /locus_tag="PLI76_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein Srp54"
                     /protein_id="HOI61977.1"
                     /translation="MLDNLGTSLKDAVKKLAGKTVIDRAAVDELVRDLQRALLSADVN
                     VKLVMELSKAIKQRALEEEPPKGMGVREHVLRIVYQELVRLMGAPGAVALGPQTILMA
                     GLQGSGKTTTTAKLARYFQRKGLRVGVICADTFRPGAYDQLKTLCDRIGIPCYGDPGE
                     KDALRIVREGLPKLRKQYEVIIIDTQGRHALEENLIEEIISINDIARADHRWLVIDAA
                     LGQQASEQARRFHEAIGIDGVLVTKMDGTARGGGALSAVAETQSGIVFIGSGETIEDL
                     ERFDPDGFISRMLGMGDLKALVERAEEAVSAEDIDVNAMLKGKFTLRDMYKQLEALNR
                     MGPLKQIMSMLPLGGMQIPDDAYDITSSKMQRYKVIMDSMTAPELDDPSVIGSSRIHR
                     IARGAGVAPEDVRDLIKYYKIMQRALKGMRGMGGGKFNMQRMMKKFGGPSR"
     gene            complement(5646..6746)
                     /gene="ftsY"
                     /locus_tag="PLI76_07820"
     CDS             complement(5646..6746)
                     /gene="ftsY"
                     /locus_tag="PLI76_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOI61978.1"
                     /translation="MFDSLKKKLQNIRTKFASNIEEAATVQPAVEQPAPPPTVEPAPP
                     PVITPAPPLPGKPLAPPAAREEKREDPTFFNRLKILVREREILLQEKDIEEPLMELEM
                     VLLENDVALPVTDEIIARMKKDLVGRHRKIGASVDDLVVATLRSALCGVLGDGLDLRD
                     YIREHERPVKILFTGVNGTGKTTTVAKVGHYLQKNGFTVVIGAGDTFRAGAIEQIRTH
                     ADRLDIKVIQHQAGADPSAVLFDAVQYAKAHEIDVVLADTAGRFHNRANLMNQLEKIR
                     RVMKPDLVVYVDEAVAGNDAVIRAGEFNRAVGTDAVVLTKADMDSKGGAAISVAHTVG
                     KPILFLGTGQGYDDIMPFTPRTVVDELLGGEA"
     gene            complement(6746..7195)
                     /gene="pfdA"
                     /locus_tag="PLI76_07825"
     CDS             complement(6746..7195)
                     /gene="pfdA"
                     /locus_tag="PLI76_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit alpha"
                     /protein_id="HOI61979.1"
                     /translation="MNQADPREIQTLQMYLNEYGQQIEILTQQLGMIEQQRIEAVAAI
                     ETLRALQESTDGVVLLPIGGGALLRVQVLDAGHVIVNIGADVSVERASADAIEYLGDR
                     ITELEAVGKKVAGSVEQLQGQATEISRRIEAAYRGIRQGEAGQGGIR"
     gene            complement(7197..7388)
                     /gene="rpl18a"
                     /locus_tag="PLI76_07830"
     CDS             complement(7197..7388)
                     /gene="rpl18a"
                     /locus_tag="PLI76_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18Ae"
                     /protein_id="HOI61980.1"
                     /translation="MENQTFEVVGACRINNAWKPYRKVVEAPNENQAREWVFADIGSK
                     HRLKRSYITIESVNVVAGE"
     gene            complement(7396..8058)
                     /locus_tag="PLI76_07835"
     CDS             complement(7396..8058)
                     /locus_tag="PLI76_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-6"
                     /protein_id="HOI61981.1"
                     /translation="MTGTIDLLGDPNIGVYARVFEDIAIVDPRVPGEFTEALGRELSV
                     DIVATTVQESGIIGSLVAGNSRGLVVSGLAAADELAVLAEHREVLLLEGSMNAAGNVI
                     LANDYVAAVHPDMDVGVAEEIGSFLGVPVIRLTLGGIKTVGMAGCATNKGILAHPRTN
                     ETEIATLERVVDLPIGLGSVNMGSGLVGTGLLANSKGYIAGSFTTGFELGRIEEVFGF
                     LE"
BASE COUNT         1379 a         2680 c         2433 g         1616 t
ORIGIN      
        1 acgaacgaga cgaaccccgc ccgggcaaag aggtcccgga cctcatcgag cccggtcagg
       61 gacgagacaa ggaggagaac ccgcccgaag ggcgcgagca cccggccggc atcggcgatg
      121 aaccgctcga tcaccaccct ccccgtcggc ccgccgtcga gggcgtactc cagccagtcg
      181 tcgatccgct cctcgggagc cgtcgggagg tagggcgggt tgaaaaggat gaggtcgaac
      241 ggccccgaga gccccgcaaa gaggtcggtc ctgaccgccg cgagaccgcg ggcgcgggcg
      301 cacgccgccg cgtgcgggtt gatatcggtc acgaccacag cggccgcccg acccgagagg
      361 ccggccgcga catacccgct cccggtcccg acctcaagca cccggtcggt ctccccgacc
      421 tcccggagcg ccgcccggag gaggagagac gaatcttcgg cgggaggata gacctcgccc
      481 gggagcatcc tcagcacccg gcaagcgtgt ttgcaaccag ggcaaactcg cacagtgtca
      541 ggttctcggg ccggcgctgc aggagatcgt cgggcagcgc cgcgatcgcc cgctcgatca
      601 cgtcgagcgg gaagaattct ttcccgctcc ggaggccttt ccggacggtc ttacgccggt
      661 gggagaagag caccctgacg acgtcggcat agacccgctt atccgctatc gggtagggcg
      721 gctcgtgcgg cgtcacccgg acaacccagg agcggacctg gggctgcggc cgaaacgcgg
      781 aaggcgagag ctcaaggagc ggtttgacgg acgcataggt ctggaccatc accgagagcc
      841 gcccgacatc cggtgtcccc ggccgcgcga tcattcgctg ggcaaactcc ttctggtaca
      901 tcaggatggc cgtctcaaac ccgatatcca gcaggcggaa cgttatcttc gaagaggccg
      961 agtacgggag gtttgcaacg acgatatcaa acggcgggat gtccaccttc ctcgcgtcgc
     1021 cggaaacgat ccggagacgc ccctcatcga tagcatcggc aaaagcgaga tcgagttcct
     1081 ccacgagagc cggatcgatc tcgaccgcga cgacattcgc accgcggtcg aggagagccc
     1141 gggtgaggat tccttcgccg ggcccgatct cgagcactcg ccggccggat acatcggcaa
     1201 acccggcgat cctctcgacg gccctccgat cgatgaggaa gtgctgatcc cgtggagcac
     1261 tcatttcagc gtgaagacat ggtacttctc gttcggatcc tcgatctcgc gcaggacccg
     1321 gctgacgatc atccgggcgg ggtgcgggag cgatttgatc cgccccgata tatccgcgaa
     1381 gctctcgaac ggcttcttct cccgctgctc gatcacctcc cacatcagtt tcttcccgat
     1441 gccggggagc aggtggagca tatggaactt cggcgtgatg ggaagagact tattgaagaa
     1501 atcgacgaac cgctgctcgt tctcgtggac gatctgctcg acaacaaacg gcagctccag
     1561 tttcgccgtc gccgtcaggt cttcgtagcc gatccgccgc ttgacacgct cgatcttatc
     1621 ccgctccgcg tcaccgatgt agacgcggtc gtggatctgg atatccgcgc ccgctttcgg
     1681 gacgagctcg agcagtttga actggtccac gccgacggca aggacaaccg gttcgcgctt
     1741 gtagacggga cgctggtcgc tcatgtatcc catcgggagg acatcaatga ctaccgcgtt
     1801 ctcttctttc ctctcagcct tcatcgacat caccccgttg gttagaagtg cgtcactacc
     1861 agctcgagga tcctgtcaag ctcttccgcg gtgaggttga accgctcttt ggcatagata
     1921 gcccgcagtt cgtcccgggt ccgcggcatc aggttcgcga tgcggtaggc aatctcctct
     1981 ttcatcttct caaactgcat gagctcgccg acgagatcgc gggctttatc ggatgatgtc
     2041 ttcgagagat gattggcatg ctcgatactt ttgcgcagct cgtaggacat ctcttctccg
     2101 gacccaagcc gggttgcctc cacggcaagg agtgtatcac gcagttccgg aagcagcatc
     2161 cgctcttcgc tgacgattcg ttttactttc atgccaatca ctactgttga gggttatacc
     2221 ttctgcgctt ttagatgttg cggtctcgca atcacctgtt tcatcgtatc gccgtccctg
     2281 acctcgagca cccatgcgcg cccgcgctgt ccgatgaccg tgccggtctt tccgtggaat
     2341 ctccggtggg gcatgccctt ctggacactc ggctcgacca cgacatgcac cttctgcccg
     2401 acctcgaagt cctgaatcac cgaggttacg ggggggatac cgcgtcttct gaggtccttc
     2461 ttgaacttat accgcgtctt cttgcgtggt ccattgtgat gtgccatgat ttactcacca
     2521 aactcgtttg ctgtttcgac cagcaccaca tcgaggctga caacacgtgc ggtgcgcccc
     2581 aggatccggg caaggctggg ctgggttctg tcgttgtcgc ccgatatcag ctctttgatg
     2641 tagaggcccg cttcgccgac gacttcgacc cagtatctgt cgtcctgtgc gcctgtacac
     2701 cggatatcga ggacatcgcg ttcccgaact ttatctgccc gtcggtgtgc cacccgcagg
     2761 ggggtgcgct gtcgtatcgt tacacctttt agctgatcga gggccgccct gaactcgtcc
     2821 tgagtcacag gaccgtcgat ctcgaccagg atcctgtatt ttttatgcgc tttgtcggac
     2881 ttaaggcttt gcaccgtctt ccgatccgcc catccggcca ggtggaccgc cactctcccc
     2941 ccggccttct cgttgatatc ctcttcaagg gccgcaaggt ccaccgaccg ccttcgcggc
     3001 gcctcgacct ccatgacgaa ggggcggccc gacccgagca tccgggcatc gatgtcctcc
     3061 cggcctgcgc cgtggaggac ggcgttctcc gcatcgaagg cttctatcac cggccggccg
     3121 atcagctcct cgacggagtc cgcgtactgt ttgccggtga agtcgcactt ctcgcacccg
     3181 gcgcccttgc aggcccggca gtcccagtgg gtctgcggga tcccccgctc gtacttcagg
     3241 tagcgcccga caaagaagac gggattgacc tgcacctcga cagtcccttc cgcgatatcg
     3301 aggatcacga cgatatcggg ccgtttcagg tcggccgtct tcccgatgag agctgatacg
     3361 gctttgccga cctcccggtt catctccgcc ttcagcggtt cggggtcgcg aagggcgaga
     3421 tcgctccaga ccatctcctc gctctccgct atgagcggcg gcactctcgt cccgataaga
     3481 aacgtcgcaa actctatccc ctgcaccgct tccacgacct ttgcggccca ggcatcggtg
     3541 cgggagagtt cgccgccgca gatccagcag gactccgact ccggctcgct atgcggctcg
     3601 tttaccgcga tctcgcgcgt gatccggagc gcccttcccc gctcctcgtt cgtcagcgaa
     3661 aacgaccgtt tgccaaagaa ccgcccgagg cagtggttgc aggtctcgcc gtactcaagg
     3721 attgctccta ccgtttctat gatatccatc cggactccct ccggtccatc tcgtttaaga
     3781 ggacggtgat ggtatgatcg gcatgcagcg accgcggccc caccgagtag cggggatagg
     3841 ccgcgatcag gtcttcctcc tcaggggtga agttcaggtg atcggagagg aggtatgcct
     3901 ccgggagcgc cggcgccgcc cggatatcct cccctgcctc atcgaggacg gcaaacggca
     3961 cctccgcgag gagtcgcgtg aacccgccgc ggcggacgta aacccccggt gtcgactcgc
     4021 ggaactcatc cccgcagggg agggcaagcg ccttcttgac cagggcggcg ctgctccgct
     4081 cgtccggcga gaggtagcga agagcgctcc ccgagaagag gacggtcttc tcgggcccgg
     4141 gctccccgca gaggatgagg taacactcga cgtcgcgccg cagatcgtgg gagagaaaga
     4201 acgaggcgtt gatgcaccgg cagaggacat ccatcctccc ggcgctcccg gcgagatcgt
     4261 tgaggctgaa cgtgccgctc gttgcggcac gatggcccac gacggcaaac cgcttcatcg
     4321 gctgggtccg ccgaacttct tcatcatgcg ctgcatgttg aacttgccgc cgcccatgcc
     4381 ccgcatgcct ttcagggcgc gctgcatgat cttgtagtat ttgatgaggt cgcggacatc
     4441 ctcgggcgcc acgccggcgc cgcgggcgat ccggtggatc cgcgagctcc cgatcaccga
     4501 agggtcatcg agctcaggag ccgtcatcga gtccatgatc accttgtagc gttgcatctt
     4561 gctgctggtg atgtcgtagg cgtcgtcggg tatctgcatg ccgccgagcg ggagcatgct
     4621 catgatctgc ttcaagggcc ccatccggtt cagcgcctca agctgcttgt acatgtcccg
     4681 gagggtgaac ttgcccttca acatcgcgtt gacgtcgata tcctcggccg agaccgcctc
     4741 ttcggcccgc tcgacgaggg cttttaagtc gcccatgccg agcatccggg atatgaaccc
     4801 gtccgggtcg aaccgttcga gatcctcgat cgtctcgccg ctcccgatga agacgatccc
     4861 gctctgcgtc tccgcgaccg ccgagagcgc gccgccgccg cgcgcggtgc cgtccatctt
     4921 cgtcacgagg acgccgtcga tcccgatcgc ctcgtggaac cggcgggcct gttcgctcgc
     4981 ctgctggccg agagccgcgt cgatgacgag ccaccggtgg tcggcgcgcg cgatatcgtt
     5041 gatgctgatg atctcctcga tcaggttctc ctcgagggcg tggcgcccct gggtatcgat
     5101 gatgatgacc tcgtactgct tccggagctt cgggaggccc tctctgacga tccggagggc
     5161 atccttctcg cccgggtccc cgtagcaggg gatgccgatc cggtcgcaga gggtcttcaa
     5221 ctggtcatag gcgcccggcc ggaaggtgtc ggcgcagatc acgccgaccc tgagcccctt
     5281 gcgctggaag tagcgggcaa gctttgccgt cgtcgtcgtc ttcccgctcc cctgcagccc
     5341 cgccatcagg atagtctggg gcccgagcgc caccgctccc ggcgccccca tcagccggac
     5401 gagctcctgg tagacgatcc gcaggacgtg ctcgcgcacg cccatcccct tcgggggctc
     5461 ctcctcaagg gcacgctgct tgatcgcctt tgagagttcc atgacgagct tgacgttgac
     5521 gtcggcggag aggagcgccc gctggaggtc gcggaccagt tcgtcgactg ccgcccggtc
     5581 gatcaccgtc ttgccggcga gtttcttgac cgcatccttc aaggatgtcc cgaggttgtc
     5641 aagcatcagg cttcacctcc caggagttcg tcgaccacgg tgcggggcgt aaagggcatg
     5701 atgtcgtcgt agccctggcc ggtcccgagg aagaggatgg gttttcccac cgtatgcgcg
     5761 accgatatgg ccgcgccgcc ctttgaatcc atatccgcct tcgtcaggac gacagcatcg
     5821 gtaccgaccg cccggttgaa ctcgccggcc cggatgacgg catcgtttcc tgccaccgcc
     5881 tcatcgacgt agacgacgag gtcgggtttc atgacccgcc ggatcttctc gagctgattc
     5941 atgaggtttg cccggttgtg gaaccggccg gccgtatcgg cgaggacgac atcgatctcg
     6001 tgcgcctttg cgtactggac cgcatcaaag aggacggcgg aagggtcggc gcccgcctgg
     6061 tgctggatca ccttgatgtc caggcggtcg gcatgggtcc ggatctgctc gatcgcgccc
     6121 gcgcggaagg tgtcgcccgc gccgatcacg accgtaaacc cgttcttctg gaggtaatgg
     6181 ccgacctttg cgaccgtcgt cgtctttccg gtcccgttca cgccggtgaa gaggatcttc
     6241 accggccgct cgtgctctct gatatagtca cgaaggtcga ggccgtcgcc aagcaccccg
     6301 cagagcgcgg agcgcagggt cgcgacgacg agatcgtcga ccgatgcgcc gatcttcctg
     6361 tgccggccca cgaggtcttt cttcatccgg gcgatgatct catccgtgac cgggagagcg
     6421 acgtcgtttt cgaggaggac catctcgagc tccatcaggg gctcctcgat atccttctcc
     6481 tggaggagga tctcccgctc ccggacgagg atctttaacc ggttgaaaaa cgttggatcc
     6541 tcccgtttct cctccctggc ggcaggaggc gccagaggtt tccccgggag agggggtgcg
     6601 ggtgtgatga ccggcggcgg tgcgggttcg acggtcggcg gcggtgcggg ctgctcgacc
     6661 gccggttgca ccgtggcggc ctcctcgata ttcgaggcga actttgtccg tatattctga
     6721 agtttcttct ttagcgaatc aaacattatc ggattccgcc ctgaccagcc tctccctgcc
     6781 ggatcccccg gtaggccgcc tcgatgcggc gggagatctc cgttgcctgc ccctgcaact
     6841 gctcgactga acctgcgacc ttcttgccga ccgcctcgag ttcggttatc cgatcgccga
     6901 ggtactcgat cgcatccgca ctggcacgct ccacggagac atcggccccg atgttcacga
     6961 ttacgtggcc ggcatcgagc acctgtaccc ggagaagggc gccgcccccg atggggagga
     7021 ggacgacgcc gtccgtgctt tcctggaggg cccggagggt ctcgatggcg gcgaccgcct
     7081 cgatgcgctg ctgctcgatc atgccaagct gctgcgtcag gatctcgatc tgctgcccgt
     7141 actcgtttaa gtacatctgg agcgtctgta tctcacgggg atctgcctgg ttcacgttat
     7201 tcaccagcta ctacgttaac cgactcgatg gtgatataac ttctcttcag gcgatgcttg
     7261 ctcccgatat ccgcaaaaac ccactctctg gcctggttct cgtttggtgc ctcgacgacc
     7321 ttgcggtacg gtttccacgc gttgttgatc ctgcacgcgc ccacaacttc aaacgtctgg
     7381 ttctccatga ctgcttcact ccaaaaaccc aaagacttcc tctatccgcc cgagttcaaa
     7441 cccggtcgta aacgatcctg caatgtatcc tttgctgttt gcaaggagac cggttccgac
     7501 aagaccgctg cccatgttga ccgacccaag gccgatcggg aggtcgacca cccgctcgag
     7561 ggtcgcgatc tccgtctcgt tcgtccgggg atgagcgaga atacccttgt ttgtcgcgca
     7621 tccggccatg ccgacggtct tgatgccccc gagcgtgagc cggatcaccg gcacgccgag
     7681 gaacgacccg atctcttcgg caaccccgac atccatgtcg gggtggacgg cggcaacata
     7741 gtcgtttgcg agtatgacgt tgcctgccgc gttcatggac ccctcgagca ggaggacttc
     7801 gcggtgctcc gcaaggaccg cgagctcatc ggcggcggca aggccgctga cgaccaggcc
     7861 ccggctgttc cccgcaacga gagaaccgat gatgccgctc tcctggacgg tggtggccac
     7921 gatatcgaca gagagttccc gcccgagggc ttcggtgaac tccccgggca cccgggggtc
     7981 cacgatcgcg atatcctcaa agacccgggc gtagacgccg atgttcgggt cgcccaagag
     8041 atcgatcgtc ccggtcatct cactcctcgg cgagctcggc ctggacctgc ccgtcctcga
     8101 acttcatc
//
LOCUS       DAOSEE010000095         8102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_3291_length_8102_cov_211.434696, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000095 DAOSEE010000000
VERSION     DAOSEE010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8102
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3291_length_8102_cov_211.434696"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..1249
                     /gene="dinB"
                     /locus_tag="PLI76_07840"
     CDS             188..1249
                     /gene="dinB"
                     /locus_tag="PLI76_07840"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOI61982.1"
                     /translation="MHVDMDSFFASIEVRRDPSLAGRPVIVGADPKGGAGRGVVSTCS
                     YEARRYGVHSGMPISRAFDLCPHGVYLPVDRNLYVRTSADIMAILSRHAGRIEQVSID
                     EAYLDVSDAGGFPAAEALAAAIKQEVRETVGLTCSVGIAPGKAVAKIASDYKKPDGLT
                     VVRPEEVAGFLAPLPVEKIPGVGRKTGEDLRQMGIRTIGDLAGHDIQALIARLGRSGI
                     SMHRLARGIDEAEVQGREGSKSIARETTFDADTADPDMLSGVVADLADDVAGTLAAAG
                     LRCRTVTVKVRYRGFETHTRSRTLPRFTADPEAIHRAASALLLPFLNGRPVRLIGVRL
                     STLEGGRTRQTAIDEFVGT"
     gene            1303..2136
                     /locus_tag="PLI76_07845"
     CDS             1303..2136
                     /locus_tag="PLI76_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61983.1"
                     /translation="MSDIWIVLLIVAIVLLFTVPVAQQQMVRTRRLRAIRDLETERRT
                     RVIALIHRQERIGFLGIPLFRYIDINDSEEVLRAIRLTPPDMPIDLVVHTPGGLILSS
                     EQIAMALRRHRGKVTIFVPHYAMSGGTLLCLAADEVAMDENAVLGPVDPRIGEYPAAS
                     ILRVPRLKPPEEIEDRTFILVDIAAKAQNQMRQFVTVLLRDRMDAERADRLSRLLTQG
                     TWTHDYPITFEEARDLGLPVTPGMPAGIYRLMDLFPQAMPRRPSVAYVPVPYQGEEKK
                     G"
     gene            2222..2653
                     /locus_tag="PLI76_07850"
     CDS             2222..2653
                     /locus_tag="PLI76_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HOI61984.1"
                     /translation="MSTSIQLNLDAFDRTLPPLCGDDPDRNGFRRLCCRCPFAVRSEG
                     SIHCERFAIPIRAREKYALRGWKEVRDTILERDGHRCAVCGGEGDLHIHHVDCDPTND
                     DPGNLVTLCGICHARVHAGLHRAGGAERVARVMKAARRRPG"
     gene            2740..3588
                     /locus_tag="PLI76_07855"
     CDS             2740..3588
                     /locus_tag="PLI76_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOI61985.1"
                     /translation="MGGTSGMGIAERLFGRGDPGDREDPDADAAVSRKAISLAQRGQF
                     DEAIACFDRVLALDPANAKMLNNKGVFLDLLGRDQEALACWEKALSIDPEFAPAWVSR
                     GMLYRRRNRFDEALACYDRAVELNPNSAVAWYNRSGVFAAMRRLDEAVACYERVLALD
                     PHFVAAWTDLGYARFLQHRHEEAIACYDRAIADDPEGVRVWSLKGGALYALGEYRKAL
                     ECFDRVLAIDPKYAAGWSMKCSVLYHLGMYRHALACADKALAINPSCELTTQVRKMLL
                     SLIEKW"
     gene            3719..4573
                     /locus_tag="PLI76_07860"
     CDS             3719..4573
                     /locus_tag="PLI76_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOI61986.1"
                     /translation="MPVDQIPIGTFSRITHLSQKALRHYDERGLLVPAARDVCTGYRY
                     YTFAQAERGLRIRHLLWLGFDLSEVEAVLAARESGDAGAIRDLFRARLAATEREALRL
                     HAIAEVLRTQDPLNGGFHMSITEPAIKDVPAQRALSLRERGTYHEAIPRLIGELLTYV
                     CPEDGRRPAARMAGPVIFICHDEEYRETDADIEVAIPIIGPVSLENSRVDIVTLPAGR
                     FVSVLHTGPYPGVGKAYERLFACMGERGLIPAGPSRELYLNNPGEVSEDELLTEIQFP
                     VQEIPPSP"
     gene            complement(4584..6044)
                     /locus_tag="PLI76_07865"
     CDS             complement(4584..6044)
                     /locus_tag="PLI76_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOI61987.1"
                     /translation="MNTPRYPSLYEVNARVRVRQIGRRATLDDIPDTWLAGLAACGFS
                     WVYLMGAWTTGDAGLRVSRSHEEWLSGYRSLIPDIEEDDICGSPFAVTAYRLHPDLGE
                     PETLERFRERLRRHDLLLMLDLVPNHTAPDHPWVRDHPDYYVHGTEEDLARRPQDYAG
                     VDPGIVLAHGRDTFSGGWPDTLQLDYSNPDLREAMIAEVQGIARKCDGLRCDMAMLVL
                     PEVFRQTWGRDMEPFWPKAIGAVRENLPEFVFMAEVYRDLEWTLQQQGFDYTYDKRLY
                     DRLRWGDARSVREHLRADPAYLDKSVHFLENHDEPRAAAVFSPDRHRAAAVLAFLSPG
                     LRFFHQGQFSGRTKQTPVHICREPEDPGDPAIEEFYRRLSASLRLPAFRDGDWRLLDC
                     NPAWEGNPSHGGYIAFAREREGERCLVTVNYAPDRGQCYVPLPWADIAGKGIRLRDLM
                     GEAAYDRDGESLLSPGLYLDIPGWGFHLFEIIPKGG"
     gene            complement(6085..7296)
                     /locus_tag="PLI76_07870"
     CDS             complement(6085..7296)
                     /locus_tag="PLI76_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HOI61988.1"
                     /translation="MEDQPAYDVVVIGGGPAGSTAACLLARFGHRVALLERETYPREK
                     VCGGCLSQKSIRFLERVFDLPVPALHEAGLLDSTGTGYALYIRDSRVLAGDLSEPLYF
                     TRRERYDAFLARMAADAGADIYEGAEVVAVDHARRTVTTSSGDRYAARVLIGADGINS
                     RVRRSLPESVVDRQRWQENLGWALELTIPRTEVKTLADGDGAIHLDADLATPQIVLAA
                     CRWGYGWVFPNREALIVGVGGLLTKNKGNQRGRFTEFLKTLGLSAFTKRRPAGYFLPF
                     GNFISSPAHEGTLLVGDAGGFASPILGEGIFYAHRTAELAAHAVDRHLTSGAPAGETY
                     TALLDRRLTPELQAEAALRGYLYGGLEGRTHALLKTFMRASSTRVIDAVQGSRSFRGF
                     RRDGDLHSAVW"
     gene            7654..>8102
                     /locus_tag="PLI76_07875"
     CDS             7654..>8102
                     /locus_tag="PLI76_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOI61989.1"
                     /translation="MSVAEQKLSHLTAASRFDLCCSGECIRTLFGASHITYNPRAEGC
                     ERMLKVLLHGACSYDCAYCDVRLQRERLSLSPDELAGTFLGFWREGLVDGLFLSSGIP
                     RDVDLVMHELVETGEILRRRGYDGYLHLKILPGAARADIHDAARVANR"
BASE COUNT         1327 a         2750 c         2669 g         1356 t
ORIGIN      
        1 ggggggcagg ggcaatggcc cgggcgctat cccgccctat gaaggccggg cggggctccc
       61 ctccctccgg gcccgacggg atcctgcatc ctctccacct ccaccaaacc ccctatatcg
      121 ccggacctcc aacactttca agagagatcc agggggagtt catcgagtgg ccgccgatcg
      181 gatcgtcatg cacgtcgata tggacagttt ctttgcctcc atcgaggtcc gccgcgaccc
      241 ctcccttgcc ggccggccgg tgatcgtcgg cgccgacccg aagggcgggg cggggcgtgg
      301 ggtcgtgagc acctgctcct acgaggcccg ccgctatggg gtgcactccg gcatgcccat
      361 atcacgggcg tttgacctct gcccgcacgg cgtctacctc ccggtggacc gcaacctcta
      421 cgtgcggaca tcggcggata tcatggcgat cctctcgcgg catgccggcc gcatcgaaca
      481 ggtgagcatc gacgaggcct acctcgacgt cagcgatgcc gggggttttc cggcggcgga
      541 ggcgcttgcc gccgcgatca agcaggaggt ccgggagacg gtggggctca cctgctccgt
      601 cggcatcgcc ccgggcaagg ccgtggccaa gatcgcgtcc gactacaaaa aacccgacgg
      661 cctgacggtc gtccgccccg aggaggtcgc ggggttcctc gcccccctgc cggtcgagaa
      721 gatccccggc gtcgggagaa agaccggtga agacctgcgg cagatgggca tccggaccat
      781 aggagacctc gcgggccacg atatccaggc gctcatcgcc cggctcgggc gatccggtat
      841 ctcgatgcac cgcctcgccc ggggcatcga cgaagctgag gtgcagggca gggaggggag
      901 caagtccatc gcccgggaga cgacgttcga tgcggacacc gccgacccgg atatgctctc
      961 gggcgttgtt gccgacctcg cggacgacgt cgcgggcacg ctcgccgccg ccggcctccg
     1021 gtgccggacc gtcacggtca aagtccgtta ccgggggttt gagacgcata cccggtcccg
     1081 gacgctcccg cggttcaccg cggacccgga agcaatccat cgggcggcat ccgctctcct
     1141 cctgccgttc ctcaacggcc ggcccgtccg gctcatcggt gtcaggctct cgacgcttga
     1201 aggcggccgc acccggcaga cggcgatcga cgagttcgtc ggcacctgac ggatcgcgca
     1261 gggcgaacct tctttacccg gagcgcccac cataccgccg gtatgtccga tatctggatc
     1321 gtgctcctca tcgtcgcgat cgtcctcctc tttacggtgc cggtcgccca gcagcagatg
     1381 gtccggaccc gccgcctgcg ggcgatccgg gacctcgaga cggagcgccg gacccgggtc
     1441 atcgccctca tccaccggca ggagcggatc gggtttctgg gcatcccgct cttccgctac
     1501 atcgatatca acgactccga agaggtcctc cgggccatcc ggctcacccc gccggatatg
     1561 ccgatcgatc ttgtcgtcca cacccccggc ggcctgatac tctcgtccga acagatagcg
     1621 atggccctcc ggcgtcacag ggggaaggtg acgatatttg tccctcacta cgccatgtcg
     1681 ggcgggacgc tcctctgcct tgccgccgac gaggtggcta tggacgagaa cgccgtgctc
     1741 ggcccggtcg acccccggat cggggagtac ccggcggcgt cgatcctccg ggtcccccgg
     1801 ttgaagccgc cggaggagat cgaggatagg acgttcatcc tggtcgacat cgcggcaaaa
     1861 gcccagaacc agatgcggca gttcgtcacg gtcctcctcc gtgaccggat ggacgccgaa
     1921 cgggcggacc gcctctcgcg cctgctcacg cagggaacgt ggacccacga ctacccgatc
     1981 accttcgaag aggcgcggga cctcggtctc cccgtcaccc ccggcatgcc cgccggtatc
     2041 taccggctga tggacctctt cccgcaggcg atgccgcgcc gcccgtcggt cgcctacgtc
     2101 ccggtgcctt accagggaga ggagaagaag ggctgatacc cccgcgatat cgtcgccatt
     2161 ctgacccgaa agacgggggt tcccagcact tatgccgtcc tgcaaccaac tattcctgac
     2221 cgtgagcacc tccatccagc tgaacctgga cgcgttcgac cgcacgctcc cccctctctg
     2281 cggagacgac cccgaccgga acggttttcg ccggctctgc tgccggtgcc cgtttgccgt
     2341 acggagcgag ggcagcatcc actgcgagcg gttcgcgatc cccatccggg cgcgcgagaa
     2401 gtacgccctg cggggctgga aagaggtacg cgacaccatc ctcgagcgcg acgggcaccg
     2461 gtgtgccgtc tgcggcgggg aaggggacct ccacattcac cacgtcgact gcgaccccac
     2521 gaacgacgac cccgggaacc tggtcacgct ctgcggcatc tgccatgccc gggtgcacgc
     2581 cggcctccac cgcgcgggcg gggcggagag ggtggcgcgg gtgatgaagg cggcacgccg
     2641 ccgtcccggc tgaccctccc gatacaccct cagggtgccc cgccggtatc aacggcaggg
     2701 gaacggttat attgtatgcc cgctaccaga tgcgtgacca tggggggaac gtcgggaatg
     2761 ggtatagcag aacgactctt cgggcggggc gatcccgggg accgggagga cccggatgct
     2821 gatgccgcag tgagcaggaa ggctatctct ctcgcccagc gggggcagtt tgacgaggca
     2881 atcgcgtgtt ttgaccgggt gctcgcgctc gatccggcca acgcaaagat gttgaacaac
     2941 aagggcgtct tcctcgacct tctcggcagg gatcaggaag cgctcgcctg ctgggagaag
     3001 gcgctctcga tcgatccgga gttcgctccg gcctgggtct cccgggggat gctgtatcgg
     3061 cgccgcaacc ggttcgatga ggcgctcgcc tgctacgacc gcgccgtgga actcaacccg
     3121 aactccgcgg tcgcctggta caaccggagc ggcgtctttg ccgcgatgcg ccggcttgac
     3181 gaggcggtcg cctgctacga gcgggtcctc gcgctcgacc cccatttcgt ggcggcctgg
     3241 accgatctcg gttacgcccg attcctgcag caccggcacg aggaggccat cgcctgctac
     3301 gatcgggcca tcgccgacga ccccgagggc gtcagggtct ggagcctgaa gggcggcgcc
     3361 ctgtacgccc tcggggagta ccgcaaagcg ctcgaatgct tcgaccgggt gctcgcgatc
     3421 gacccgaagt atgccgccgg gtggagcatg aagtgcagcg tcctctatca tctcggcatg
     3481 taccgccatg cgcttgcgtg tgccgacaaa gcgcttgcga tcaatccttc ctgcgagctg
     3541 accacccagg tccgtaagat gctcctctcc ctcatcgaga agtggtgagg gggcccgcac
     3601 gggacattcg aaaggttgac gccgggcgcc gtccaaccaa gagaccgtga ggaaactctc
     3661 ccccggggga gactccgacc gcctattacc cggcggtgcc aacaggtgaa ttgtatccat
     3721 gccggtcgat cagatcccta tcggtacgtt ctcgcggatc acccatctat cgcagaaggc
     3781 gctccgccac tacgacgagc ggggacttct cgtccctgcg gcgcgagacg tgtgcacggg
     3841 gtatcgctac tacacctttg cccaggccga gcggggcctc cgcatcaggc acctgctctg
     3901 gctggggttc gacctctccg aggtcgaggc ggttctcgcg gccagggaga gcggcgatgc
     3961 cggagcgatc cgcgatctct ttcgcgcgcg cctcgctgca accgagcggg aagctctacg
     4021 gctgcatgcg attgcagagg tcctgcgaac acaggatccc ctgaacggag gattccatat
     4081 gtcgatcacc gaacctgcca tcaaggatgt ccctgcccag cgtgcgctca gcctccggga
     4141 gcggggtacc taccacgaag cgatcccgag actgatcggg gaactactca cgtatgtctg
     4201 tcccgaagac gggcggcggc ccgctgcccg gatggccggg ccggtcatat tcatctgcca
     4261 cgacgaggaa taccgggaga cggatgcgga tatcgaggtc gcgatcccga tcatcgggcc
     4321 ggtcagcctt gaaaactcac gggtcgatat cgtgaccctt ccggccggcc ggttcgtctc
     4381 ggtgctccac acgggcccct atccgggtgt cgggaaggcc tacgagcggc tctttgcctg
     4441 catgggcgag cgcggtctta tccccgccgg cccctcgcgg gaactctacc tcaacaaccc
     4501 cggagaggta tcggaggacg agctcctgac ggagatccag ttcccggtgc aggagatccc
     4561 tccctccccc tgacgggagg aggtcaccct ccctttggta tgatctcgaa caggtggaac
     4621 ccccacccgg gtatgtccag gtagagcccg ggcgaaagaa ggctctcccc gtcgcggtcg
     4681 tatgctgcct cccccatgag gtcgcggagc cggatgcctt tccccgctat atcggcccac
     4741 gggagcggga cgtaacactg gcccctgtcg ggcgcgtagt tgacggttac gaggcagcgt
     4801 tctccctccc tctcccgggc aaaggcgatg tagccgccgt gggacgggtt tccttcccag
     4861 gcggggttgc agtcgaggag ccgccaatcc ccgtcccgga acgccgggag ccggagagag
     4921 gcagaaagcc gccggtagaa ctcttctatg gccggatcgc ccgggtcctc cggctcccgg
     4981 cagatgtgga cgggagtctg cttcgtgcgc ccggaaaact gcccctggtg aaagaaccgg
     5041 aggccgggcg agaggaaggc gaggacggcg gcagcccggt ggcggtcggg cgaaaagacg
     5101 gctgccgccc ggggttcgtc gtggttctcg aggaaatgga cggatttatc caggtatgcc
     5161 ggatccgccc ggaggtgctc ccgcaccgac cgtgcgtcgc cccaccgcag tcggtcgtag
     5221 agccgtttgt cgtaggtgta gtcaaacccc tgctgctgga gcgtccactc caggtcccgg
     5281 tagacctccg ccatgaagac gaattccgga aggttctcgc gaacagcacc gatcgctttc
     5341 ggccagaaag gctccatatc cctcccccag gtctgccgga agacttccgg gaggacgagc
     5401 atcgccatgt cgcaccggag gccgtcgcac ttcctcgcga tcccctgcac ctcggcgatc
     5461 atcgcttccc gaagatccgg gttactgtaa tcgagctgca gggtgtcggg ccagccgccg
     5521 gagaacgtgt cccggccgtg ggcgaggacg atccccggat ccaccccggc atagtcctgc
     5581 ggccggcggg cgaggtcttc ctccgtgccg tggacgtagt agtcggggtg atcgcgcacc
     5641 caggggtgat cgggcgccgt gtggttcggg acgaggtcga gcatgaggag gaggtcgtgc
     5701 cggcgcagcc gttcgcggaa ccgctcgagc gtctccggct ctccgaggtc cgggtggagg
     5761 cggtaggcgg tgacggcaaa aggggagccg cagatgtcgt cctcctcgat atcggggatc
     5821 aggctccggt agccgctgag ccactcctcg tgtgagcggg agacccggag cccggcatct
     5881 cccgtcgtcc aggcgcccat gaggtagacc caggagaatc cgcaggcggc aagccctgca
     5941 agccaggtgt cggggatatc gtcgagggtg gcgcggcggc cgatctgccg gacccggacg
     6001 cgggcgttga cctcgtagag ggaggggtag cggggggtgt tcatgcgggg ctctcttcgg
     6061 tcctcgggac gatagaggta tccctcacca gacggcggag tggagatcgc cgtcccgccg
     6121 gaacccccga aacgaccgcg aaccctggac ggcatcgatg acccgggtgc tcgatgcccg
     6181 catgaaggtc ttgaggagcg catgcgtccg cccttcgagg ccgccgtaga ggtagccccg
     6241 gagcgccgcc tccgcctgca gctccggtgt gaggcggcgg tcgaggaggg cggtgtaggt
     6301 ctcgccggcc ggcgcaccgg acgtgaggtg gcggtcgacg gcgtgggcgg caagttctgc
     6361 ggtccggtgg gcgtaaaaga tcccttcgcc gaggatgggg cttgcaaacc ccccggcatc
     6421 gccgacgagg agcgtcccct catgggcagg cgaggagatg aagtttccaa acggcaggaa
     6481 gtatcccgcc ggccgcctct tcgtgaacgc cgagaggccg agggtcttga ggaactctgt
     6541 aaatcggccg cgctggttgc ctttgttctt cgtgagcagc cctcccaccc ccacgatgag
     6601 cgcttccctg ttcgggaaga cccacccgta gccccaccgg caggcggcaa ggacgatctg
     6661 cggcgtcgca aggtcggcgt cgaggtggat cgcgccgtcg ccgtccgcga gcgtcttcac
     6721 ctccgttcgc gggatcgtga gttccagagc ccacccgagg ttctcctgcc atcgctgccg
     6781 gtcgacgacg ctctccggga gggagcgccg gacccggctg ttgataccgt cggccccgat
     6841 gaggacccgg gcggcgtatc ggtccccgct cgacgtcgtg acggtgcgcc gggcgtggtc
     6901 gacggcgacg acctctgcgc cttcgtagat atccgccccg gcatccgccg ccatccgggc
     6961 gaggaaggca tcgtagcgct cccgccgggt aaagtagagc ggctccgaaa ggtccccggc
     7021 gaggacccgg ctatccctga tgtagagcgc atacccggtc ccggtcgaat cgagcaggcc
     7081 cgcttcgtgg agtgcgggga ccgggaggtc aaagacccgt tcgaggaacc gaatggactt
     7141 ctggctgagg cagcccccgc agaccttctc ccgcggatac gtctctctct caaggagcgc
     7201 cacccggtgc ccgaaccgcg cgagcaggca ggcggcggtg ctcccggcag gcccgccccc
     7261 gatgacgacg acatcgtatg ccggttgatc ctccatactt ctctctcctc ggcgacgata
     7321 gggtccggcg cagactatat tcctttcccc ggagggagta tggtgtgcgc cgcgattgaa
     7381 tagcgtttct caagaggggt atttacgggc tccccttttg ccgcgaaggc tccctccgga
     7441 ggctgctttc ctctccccgg cagcagcggg gatatcgccg gcccgtcgtt cggaacgggg
     7501 ttctcgcgaa tatccgcgcg tagcccccgg gcgatcgccg ccgaaggccg gttgtataac
     7561 ccggggtaga gtagaaagaa ctgcacgcca ggttccgttc actttcaccc cgcacccgac
     7621 aggctcatct tctatcgggc cgatgcttcc ggtatgtccg ttgcggagca gaaactcagc
     7681 catctcaccg ctgccagccg gttcgacctc tgctgctccg gggagtgtat ccggacgctc
     7741 ttcggtgctt ctcacatcac ctacaacccc cgggccgaag gatgcgagcg gatgctcaag
     7801 gttctcctcc acggcgcctg ctcctacgat tgcgcctact gcgacgtccg cctccagagg
     7861 gagcgcctct cgctctcccc ggatgaactt gccggcacct tcctcggttt ctggcgggag
     7921 gggctcgtcg acggcctctt cctgagctcc gggattcccc gcgacgtcga cctggtgatg
     7981 cacgaactcg tggagacggg agagatcctc cgccggcgcg gctacgacgg ctatcttcac
     8041 ttaaagatcc tccccggcgc ggcccgggcg gatatccacg acgccgcccg cgtggcaaac
     8101 cg
//
LOCUS       DAOSEE010000096         8094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_3295_length_8094_cov_218.138450, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000096 DAOSEE010000000
VERSION     DAOSEE010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8094
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3295_length_8094_cov_218.138450"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            417..2150
                     /locus_tag="PLI76_07880"
     CDS             417..2150
                     /locus_tag="PLI76_07880"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOI61990.1"
                     /translation="MQPIASIIRDIIGRRPARIAPVAIIAAVLIACCGCTAAVNAGND
                     GEAARDISDIQADPSHSSSAEPDYSVVFPDDRVNVMTIAITPENWRKMLDNMTELHGE
                     FGGGATGGMPAAPAARGNMQPGAGRGMGGISDVDPVYVPANVLFEDKVWTDVGIRFKG
                     QMSLGSTWREGSYKISFKLNFDKFEDENPAIKNQRFYGFDELNLQSGYTDDSLMRDMI
                     VPSIFRESGVPAPHTAFYRVYLDYGEGPVYFGLYTMIESVEDTVIETQFENDSGNVYK
                     PEGVGATFARGTFDTASFEKKTNKGDGNWTDVETLYTVLNSETRTTDPEAWRAELETI
                     LDVDEFLRWLATNTVIQNWDTYGGNSRNFYLYTDPADGRIVWIPWDNNFALNGNMGTG
                     NRGDLPTDGALQMNGPGADPAREFGRPIPAGADALQMNGPPADLTREFGRPIPADGAA
                     PGNRTGMNPAAGFGGGMGRTLSLDLGDVGENWPLIRYLADDPVYYTQYQEYLEMVVTT
                     SFEPTDMEETYRYYHALIEPYVTGPDGEQAGYTHLRSAEAFEASLDALIEHVYDRHDA
                     VMDYLSTERAL"
     gene            2168..2995
                     /locus_tag="PLI76_07885"
     CDS             2168..2995
                     /locus_tag="PLI76_07885"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HOI61991.1"
                     /translation="MEGTTMDSCMQSDLDDPGDCWFPSVVRDIESISLAEVAEAKTRL
                     LNRVESKHLMTVGQCIRLIQAISGSYRVLEVHDARVGRYETQYYDDGMFTTYIQHHNG
                     KGNRYKLRLRHYESSGETYLEVKKKSNKGSTEKQRMKTSWPPAGFLPEEERFLKAAFP
                     YDHQEFRPVLRTIYERLTLVSTEFPERITFDTAVLFSNGQRSISYPGLVIGEVKREKN
                     AKNSSAQRAMRSMGIQERAFSKYCVGVSLLYGQLKHNRFKPNLRFIAGLSQGGRQPC"
     gene            2989..3576
                     /locus_tag="PLI76_07890"
     CDS             2989..3576
                     /locus_tag="PLI76_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HOI61992.1"
                     /translation="MLTGDLTLFVAGFVLNFLAAFVIVRFVYYPKGRCNNYVFTFLAF
                     NTVIYFIMGLFTSVELSIGAGFGLFALFSILRYRTETVPIREMTYLFVMAALPVLNSV
                     LWGFARYDEMLVANILVVLTIWILDKAWGFNREQHQMDVIYEKIDLIPRERREEMLAD
                     LRERTGLNVYEVEVLSINFLRDTAEIRITYSNNKG"
     gene            complement(3635..4390)
                     /locus_tag="PLI76_07895"
     CDS             complement(3635..4390)
                     /locus_tag="PLI76_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOI61993.1"
                     /translation="MTAEVIPGLRVIGMNEYEASVYSTLVGLQKATARDVHEISGVPR
                     GRIYEILNDLARRGFIGVEEGSPTSYYVQDIDVVFDRLKDDYVRSLDETRETLKSLSV
                     KPRLPPDSFFILRSEWAIENHISSLFRRVKKSMVILCYNPEFLQRYYTIIKPLERKID
                     LYIIVRKREEYAGIKLPIYEARGTVIDLLDNRPVNESVMEGWREEECGILVDGRDFFA
                     VVTAGSGRYAVVGSDMPLIDYLQKTVVEQLREA"
     gene            4976..5398
                     /locus_tag="PLI76_07900"
     CDS             4976..5398
                     /locus_tag="PLI76_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI61994.1"
                     /translation="MNCTSTHSTGSRSGSDKPLRRKSVYHNKFCQEPQFEETDQVWTE
                     AGFLPPTGCRRTVALLHRLAMNEEPVASLLSLVLTATAVGVDNMGNDYRILDQTQLRR
                     TAGEYGIDPAGRSDEEIARAVALAIISEYGESAPEEDA"
     gene            5395..7659
                     /locus_tag="PLI76_07905"
     CDS             5395..7659
                     /locus_tag="PLI76_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RND family transporter"
                     /protein_id="HOI61995.1"
                     /translation="MKNPYERLANAINNHTLTTFGIIVTVFVLALFGLTMVTMETGDD
                     TYIDKNTPRGALLAHYKDTYGSDAIMLIYEADSVRSPDALRYIDHLQEDLRNERYVDS
                     VSGVVDLLKQANDGTLPSSKAEIDAIIGETPPEMVERMLPSNMMTISVISLEPGVSKQ
                     IQEQVLGNIGSIIGVSEPPPGLSVTVSGTPAFSKEMGEAMGAEMGTLILAAMILMTIA
                     VMLLFSHVRYRLLPIGVVTVGLILTFGLMGLFGIPVSMVVIAAFPVLIGVGIDYAIQF
                     QARFDEETRRSTLPEAVRTTVVHAGPSILIAMTATALGFVAMFFTPVPMIADFGKVCA
                     IGVASCYLAAFIIVPVFGILTNYRPKKDTDLSNPAPESEQSFIERYEHLIGKMAYTIA
                     KHPLPVLLIFGMVAVGGYYLDERVPISADEKTFVPDDMPALENMGKITRTMGATSTIP
                     VVVRGDNVLFPETLAWIDRFGTYELEHNDKMTGVTSIATLIRDYNGGVLPATKGEVDE
                     AVARLPEATQKRYLNGNMEAVLEFSTVSMEMDTQKSFIEGIRSDIAWSELPAGVTVQI
                     TGTGEMFTAIMDEISESKMFMTLLGFAFILAFLLLVYRRVGAASPLIPIAFVVGWCGA
                     IMYLLGLDYTPLTAVLGSMSIGVASEYTIVIMERCEEELAKGVGYLDAIQHGVQKIGM
                     AITVSGVATISGFAALTVSSFNIISNFGVVTVLTVGFSLVGAIIALPAVLSLMYRYAG
                     SSRAPLPTGAGVTG"
BASE COUNT         1771 a         2486 c         2328 g         1509 t
ORIGIN      
        1 cggcacgatc ctctttgcag gcagggtcgt aaacccggtg gggccgtaag gggcgcagga
       61 ttactcccgc gcccccttat ctgaacggag cgtgcacgct ttcaccgcac tccgctcaag
      121 cacttcacaa cccgagtttc gggcggcaat aagcgaagcc cttcgcatat tgcctttgca
      181 gtcggggtct gtagcctgtt aacaactccc tgtcagtatg cttctcacct tgcggcgtcg
      241 ttgaatgaca tactttcaca ggtatacctc ccctcgtcgc cgtgaaacac caggagtaag
      301 tccgcgccct ttcgggtcgg gacagaagtc atccctgtcg ctggcgctcc acaccaggaa
      361 acgattgcat acaatacgta aagtattatt aacattatgt ctaaacatac agacatatgc
      421 aacccatcgc atcgatcata cgcgatataa taggaaggag gccggcgaga atcgcacccg
      481 tggccatcat cgcagctgtt ctcatcgcct gctgcggttg caccgccgca gtaaatgcgg
      541 gaaacgatgg tgaggcggcc cgagatatca gtgacatcca ggccgacccc tcgcatagca
      601 gtagcgcaga accggactac tccgtggtat ttcccgacga cagggtcaac gtgatgacca
      661 tcgccatcac cccggaaaac tggcggaaga tgcttgacaa catgaccgag cttcacggcg
      721 agttcggtgg aggcgccacg ggaggtatgc ctgccgcgcc ggcggcccgg ggcaacatgc
      781 agcccggcgc aggccggggc atgggaggca tctccgatgt cgacccggtc tatgtccctg
      841 caaacgtctt attcgaggat aaggtctgga cagacgtcgg tatccggttc aagggccaga
      901 tgtcccttgg aagcacgtgg agggaggggt cgtacaagat ctcgttcaag ctcaacttcg
      961 ataaattcga ggacgagaac cccgcgatca agaaccagcg gttctacggg ttcgacgagc
     1021 tcaacctgca gagcggctac accgacgact cgctgatgcg ggacatgatt gtcccgtcta
     1081 tcttccggga gtccggagtc cctgcgccgc acaccgcgtt ctaccgggtc tatctcgatt
     1141 acggagaggg accggtctat ttcggactct acacgatgat cgagagcgtc gaggacacgg
     1201 tgatcgagac ccagttcgag aacgactccg gcaacgtcta taaacccgaa ggcgtcggag
     1261 ctacgtttgc ccggggcacg ttcgatacgg caagtttcga gaagaagacc aacaaaggcg
     1321 acgggaactg gaccgatgtc gagacgcttt acaccgtcct gaactcggag acccggacga
     1381 ccgacccgga ggcgtggagg gctgagctcg agacgatact ggacgtcgat gagttcctcc
     1441 gctggcttgc aacgaacacc gtcatccaga actgggacac ctacgggggc aacagcagga
     1501 acttctacct gtatacagat cccgccgacg gcagaatcgt ctggatcccc tgggacaaca
     1561 acttcgccct caacggcaac atgggcaccg gcaaccgggg cgatctcccg acagacggcg
     1621 ccctgcagat gaacggcccc ggggcggatc ctgcacggga attcggccgc ccgattccgg
     1681 cgggcgccga cgccctacag atgaacggcc ccccggcgga tcttacacgg gagttcggtc
     1741 gcccgattcc agcagacggc gccgcaccgg ggaaccgaac cgggatgaac ccggccgcgg
     1801 ggttcggcgg cggcatgggc aggaccctct cactcgatct cggagatgtc ggggagaact
     1861 ggccgctgat acgctatctg gccgacgacc cggtgtacta cacgcagtac caggaatacc
     1921 tggagatggt cgtgacgacg tcgtttgaac cgacggatat ggaagagacc taccggtatt
     1981 accatgccct gatcgagcct tacgtgaccg gccccgacgg agaacaggca ggttacacac
     2041 acctgaggag cgccgaagcc ttcgaagcct cccttgatgc gttgatcgaa catgtctacg
     2101 accgtcacga tgcggtcatg gattacctct ccaccgagag ggcattataa atcgagagca
     2161 agttgccatg gaaggaacta cgatggactc ctgcatgcaa agcgatctcg acgaccccgg
     2221 ggactgctgg ttcccgtcgg tcgtccggga tatcgagagc atctccctcg ccgaggtggc
     2281 ggaggcgaag acccggctgt tgaaccgggt ggagagcaaa cacctgatga cggttggaca
     2341 gtgcataaga ttgatacagg cgatatccgg ctcgtacagg gttctggagg ttcacgatgc
     2401 cagggtcggc agatacgaga cccagtatta cgatgacggt atgttcacca cgtatatcca
     2461 gcaccacaac ggaaagggca accgctacaa actgcggttg cgtcattacg aatcatcggg
     2521 agagacctac ctcgaggtga agaaaaaaag caacaaaggt tccaccgaga agcagcggat
     2581 gaagacatcc tggccgccgg ccgggtttct gcctgaggaa gagcgtttcc tcaaggcagc
     2641 ctttccctac gaccatcagg agttccgccc cgtcctcaga actatctacg agaggttaac
     2701 cctggtctca acggagttcc ccgagcgtat cacgttcgat acggccgtcc tgttcagcaa
     2761 cgggcagcgg agcatctcgt atcccgggct cgtcatcggt gaggtgaagc gcgagaagaa
     2821 cgcaaagaat tcctcggcac agcgggccat gcgctcaatg ggtatccagg agagagcctt
     2881 ctcgaagtac tgcgtcggtg tctcgctgct ctacggtcaa ctgaagcata acaggttcaa
     2941 gccgaacctg cgcttcatcg ccgggctctc tcagggaggc cgccagccat gctgaccggc
     3001 gatctcaccc ttttcgtcgc aggcttcgtc ctcaacttcc ttgccgcgtt cgtcatcgtc
     3061 cggttcgtct actaccccaa aggccggtgc aacaactacg tcttcacgtt cctcgccttc
     3121 aacacggtga tctacttcat catggggctg ttcaccagcg tcgagctctc gataggagca
     3181 ggtttcggcc tttttgcgct cttctcgatc ctgcgctaca ggacggaaac ggtcccgata
     3241 cgcgagatga cctacctctt cgtgatggcc gcgctgcccg tactgaactc ggttctctgg
     3301 gggtttgccc ggtacgacga gatgctggtc gcaaacatcc tcgtcgtcct gacgatctgg
     3361 attctcgaca aggcgtgggg gttcaacaga gagcagcacc agatggatgt gatctacgag
     3421 aagatcgacc tgataccgcg agagcggcgg gaagaaatgc tcgccgatct cagggagaga
     3481 accggattga acgtttatga ggttgaggtg ctgagtatca actttctgcg ggataccgca
     3541 gagatcagga tcacttactc gaacaacaaa ggataatgcc tttttcgcgg cgagccggtg
     3601 cggggtaagt cccggccggc cggcgcctcc cccctcacgc ctcccggagc tgctcgacga
     3661 ccgtcttttg caggtaatcg atgagcggca tatccgaacc gaccaccgcg tacctcccgg
     3721 accccgcggt gacgacggcg aagaaatctc gaccgtcaac caggatcccg cactcttcct
     3781 ccctccagcc ctccatcacg gactcgttca ccggccggtt atccagaagg tcgatcacgg
     3841 ttcctctggc ctcgtagatc gggagtttga tccccgcata ctcctccctt ttccgcacga
     3901 tgatgtagag gtcgatcttg cgctcgaggg gtttgatgat cgtatagtat ctctggagaa
     3961 actcggggtt gtaacagagg atcaccatgc tcttctttac ccggcggaag agcgatgata
     4021 tatggttctc gatcgcccat tcgctccgga ggatgaagaa cgagtccggg gggaggcgcg
     4081 gttttaccga gaggctcttc aacgtttcgc gcgtctcgtc aagggagcgg acgtagtcat
     4141 ctttgagtcg gtcgaagacc acatcgatat cctgtacgta gtaggaggtc ggagaaccct
     4201 cttccacacc gatgaacccc cgccgggcaa gatcatttaa tatctcgtag atccgccccc
     4261 ggggaacacc gcttatctcg tggacgtcgc gcgcggtcgc tttctgcagc ccgacgagcg
     4321 tagagtagac actcgcttcg tactcgttca tcccgataac ccggagcccg gggataactt
     4381 cagcagtcat tgcaactcaa ttaaggtgca acacatttat cgtttgcgca gaaagatgga
     4441 tagaacagcg agagaagatc cgtctctcgc aagatcgccc cgtagcagcc gcctgctgga
     4501 agccctgcaa accggccccc acgctgaaag agccggaacg ccgggcatac tcaccttcga
     4561 gaaaggtcgc tgcgatgagg acaaagagag cagcacaaag cacaaaagag cgataccacc
     4621 tgaaccaaca gaatcagatg agtgagacgc atcgccctca caaggcaggg agatccgtca
     4681 tcaacatacc ctagagcgta tcctgcggca acgacggcgg gcccgggtgt ggccgggtcg
     4741 ccgcccgcac cgcccctgtc ccgggcaccg gaaatatcct tccttttctg acccaatccc
     4801 ggggggcatc ccaccggaga gggcttgtgc cgcacgacga ccgatcctaa agggccctgc
     4861 ggggaacctc gctccgggag tagggacgag ccctcgccgg agttgcacta aattcatctt
     4921 tgaccttttg aaacaactac tccgataata tagcccaggt acagcgagga aagacgtgaa
     4981 ctgcacatca acccacagta ccggatcccg tagcggttcc gacaaaccgc tacgccggaa
     5041 atcggtctac cacaacaagt tttgccagga gccacagttt gaggagaccg atcaggtctg
     5101 gaccgaggcc gggttcctcc ccccgacagg ctgtcgacgc accgtagccc tgctccatcg
     5161 ccttgccatg aacgaagagc ccgtagcatc cctgctctcg ctcgttctca ccgcaacggc
     5221 ggtaggagtc gacaatatgg gcaacgacta ccggatcctc gaccagacgc agctccggcg
     5281 aaccgccggg gagtacggaa tcgatcccgc cggcagaagc gatgaagaga ttgcacgcgc
     5341 cgtggccctg gcaatcatca gcgagtatgg ggagagcgca ccggaggagg acgcatgaag
     5401 aacccttacg aacggctcgc aaacgccatc aacaaccaca ccctgaccac ctttggaatc
     5461 attgtaaccg tcttcgtcct ggcgctcttc ggactcacga tggtgacgat ggagaccggc
     5521 gacgacacct acatcgacaa gaacaccccc cgcggcgcgc tcctcgccca ctataaggac
     5581 acctacggct cagacgcgat catgctcata tacgaggcgg acagcgtccg gagccccgat
     5641 gccctcaggt atatcgatca cctccaggag gacctcagga acgagcgcta cgtcgacagc
     5701 gtctccggcg tcgtcgacct cttgaaacag gcaaacgacg gcaccctccc gtcgtccaag
     5761 gcggagatcg atgcgatcat cggggagact ccgccggaga tggtcgagag gatgctgcca
     5821 tcgaatatga tgacgatcag cgtcatcagc ctcgagccgg gcgtctccaa acagatccag
     5881 gagcaggttc tcgggaacat cgggtcgatc atcggcgtat cggagccccc accgggtctt
     5941 tcggtcacag tctccgggac tccggcgttc tcaaaagaga tgggcgaggc gatgggggcg
     6001 gagatgggca cgctgatcct tgcggccatg atccttatga ccatcgcggt gatgctcctc
     6061 ttctcccacg tccgctaccg ccttctcccc atcggggtcg tgaccgtcgg gctcatcctg
     6121 acattcggac tcatgggcct cttcggaatc ccggtcagca tggtggtgat tgcggcattc
     6181 ccggtgctca tcggggtcgg gatcgactac gcgatccagt tccaggcccg gttcgacgag
     6241 gagacccggc gcagcaccct cccggaagcc gtacggacca ccgtcgtcca tgcgggccct
     6301 tcgatcctga tcgcgatgac ggcaacggcg ctcgggttcg tcgcgatgtt cttcaccccg
     6361 gtgccgatga tagccgactt cggaaaagtc tgcgccatag gcgtggcctc ctgctacctt
     6421 gcggcgttca tcatcgtccc ggtctttgga atcctcacga actaccgacc gaagaaagat
     6481 accgatctct ccaaccccgc ccccgaatcg gagcagtcgt tcatcgaacg atatgagcac
     6541 ctcatcggaa agatggccta taccattgca aaacacccgc ttcccgtcct cctgatcttc
     6601 ggcatggttg cggtaggcgg ttattacctt gacgagagag ttcccatcag cgcagacgag
     6661 aagaccttcg ttcccgacga catgcccgcc ctcgagaata tgggaaagat cacccggacg
     6721 atgggggcaa cgagcaccat cccggttgtc gtgagggggg acaacgtcct ctttccggag
     6781 acactcgcct ggatagaccg attcgggaca tacgaactcg agcacaacga caagatgacc
     6841 ggggtaacga gtatcgcaac cctgatccgg gactacaacg gcggggtcct gcccgcgacg
     6901 aagggggagg tcgatgaggc cgtcgcccgg ctcccggagg cgacccaaaa gcgctacctc
     6961 aacgggaaca tggaggcggt gctcgaattc tcgacggttt cgatggagat ggacactcag
     7021 aagtcgttca tagaagggat acggagcgat atcgcgtgga gcgagctccc cgccggtgtg
     7081 acggtgcaga tcaccggcac cggagaaatg ttcaccgcca tcatggacga gatcagcgag
     7141 tccaagatgt tcatgaccct gctcgggttc gccttcatcc tcgccttcct gctcctcgtc
     7201 taccggagag tcggcgccgc atcgccgctc atcccgatcg ccttcgtcgt gggctggtgc
     7261 ggggcgatca tgtacctcct cggcctcgat tacaccccgc tgaccgccgt tctcggatcg
     7321 atgagcatcg gcgtggcctc ggagtatacc atcgtgatca tggagcggtg cgaggaggaa
     7381 ctcgccaagg gcgtaggata cctcgacgcc atccagcacg gagtgcagaa gatcgggatg
     7441 gccatcacgg tatccggggt ggcaacgatc tccgggtttg cggcgctcac cgtctcgagt
     7501 ttcaacatca tcagcaactt cggggtcgtg acggtcctca ccgtcgggtt ctcgctcgtg
     7561 ggcgcgatca tcgcgctgcc ggcagtgctc tcactcatgt accgctacgc gggaagttca
     7621 cgggccccgc tccccaccgg ggccggggtc acgggctgat acccttttcg tgtgaagata
     7681 cccttcccct tttttatccg gtatgtctgt ggccccacgg gcatcatgtc gtttttccgg
     7741 cggatggcgt gccccgggtg cgggggcgtc atcaccggtc ccacaagaac tataccctga
     7801 caacgagtta catatagcga agagggctgt gagctgctca aaccaccctg cagcgccgga
     7861 acctgccggg actccagcga accccgggct ggagacccat agagagacgt catttgaggg
     7921 accattcccg gagacacgcg atccgtaacc tccggtcaac agaatgacga cagacatgct
     7981 catgcgtacc ccccggcgtg catcgcagag cacggggaag agacgccgga ggaacataca
     8041 tgaagaaccc ctaccaatgg ctcgccgacg ccatcaacaa ccacacctgg gccg
//
LOCUS       DAOSEE010000097         8069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_3308_length_8069_cov_300.825181, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000097 DAOSEE010000000
VERSION     DAOSEE010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8069
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3308_length_8069_cov_300.825181"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="PLI76_07910"
     CDS             complement(<1..871)
                     /locus_tag="PLI76_07910"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit B"
                     /protein_id="HOI61996.1"
                     /translation="MTKTVTDVVCPFCGTLCDDLEVVVSDDGKKLFEVYNACAIGAEK
                     FLHSQAADRLTRPRMRQDDGSWKEISYDEAIEYTARMLADAKKPLMYGWSSTNCEAQA
                     VGSEIGEMVGAVMDNTATVCHGTSLIAVQDIGIPSCTLGEVKNRADRILFWGCNPAHA
                     HPRHMSRYSIFPRGFFTGKGHTGRKMIVVDPRPTDTARIADVHLQIEQGRDYELLDAF
                     RVAFKGEKLPDVVAGIPKEKIYEAADMLKSGRFAIIFFGMGVTQSLGKNHNIDAAIAV
                     TRDLNEYTKAAIMP"
     gene            complement(868..1269)
                     /locus_tag="PLI76_07915"
     CDS             complement(868..1269)
                     /locus_tag="PLI76_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HOI61997.1"
                     /translation="MAIRVNLITGRTIQQGVSMEAGKEKPAYTAACGIIELDPADFKK
                     LGAFRNTTVRVTSQYGSVVVKAVEATQGPHPGVAYIPMGPWANRVINPNTYSTGMPTF
                     KGTPVTVEVAKNEPVLDSLELVRKGCRGELA"
     gene            complement(1297..1722)
                     /locus_tag="PLI76_07920"
     CDS             complement(1297..1722)
                     /locus_tag="PLI76_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOI61998.1"
                     /translation="MADENWKPKIIAIICNWCSYAGADLAGGARIQYPPDIRAVRVMC
                     TGRVDPLFILKAFQDGADGVLVSGCHFGDCHYLEGNYKAAKRMFLLKSVLKNIGLDDK
                     RLRMTFVSASEGAKWGHVMEDVVRTITELGPSPLKELAR"
     gene            complement(1723..3747)
                     /locus_tag="PLI76_07925"
     CDS             complement(1723..3747)
                     /locus_tag="PLI76_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOI61999.1"
                     /translation="MAEVKKYEEPRIGVFVCHCGTNIAGSIDVKAVMEYARTLPNVIV
                     ADDYQYMCSTPGQNKIAEAINEQKLTGIVVAACSPRLHEPTFRNATKEGGLNPFRFEM
                     ANIREQNSWVHMHQPEEATAKAKDAVRIAVAKASLLEDLIPKSVPVEKTAMVVGAGVG
                     GMQAALDLASAGIKTYLVEKEPTIGGKMSQLDKTFPTLDCSQCILTPKMVDVYRHEGI
                     ELLTYTEVEAVEGYIGNFDVTLRKKARGVLTPDEATARGIIGGGCTGCGDCTDVCPVI
                     KPNPFELGMAPRKAIYIYHPQVSPLIYTVDFDACVKCGLCVEACGEERKAIDLDAKDE
                     LMNVKVGTVVLALGYDIFPIEKKFEWGYKHYDNVITSLEFERLICASGPTGGHLIRPS
                     DGETPKRIAFVLCAGSRDNTGVGKPYCSRFCCMYSLKHAHQIIEKIPGAIPYIFYMDI
                     RSFGKMYEEFYYRIQDEGAKFIRGRVANILEDPVTKNLHVNAEDTLLGRPIDMEVDLV
                     VLAAAVQPSADTEKTRRLFGVSCSQDGWLLEAHPKLNPCGTTTAGVYLAGVCQGPKDI
                     PDTVAQAEGAASAASIPIHRGEVELEPYFAVCMEDKCAGCGLCVNLCPYQALSLIEKD
                     GRTVMHVTEAKCKGCGTCGGFCPGGAIWMQHFATPQIVAQIDAFLLGGEQ"
     gene            complement(3759..4658)
                     /gene="hdrB"
                     /locus_tag="PLI76_07930"
     CDS             complement(3759..4658)
                     /gene="hdrB"
                     /locus_tag="PLI76_07930"
                     /EC_number="1.8.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase subunit B"
                     /protein_id="HOI62000.1"
                     /translation="MSNRHQYAFFLGCIAPNRYPGIEAAAIKTSANVGIDLLPLKGAS
                     CCPAPGAFGSIDLNVWYAMAARNVVLAEQMKTDITLICNGCYKSIWEVNHKLKHNDEL
                     RDGVNEVLKEIDMEFKGTVDVWHLAELYYDPKIVGIKKLADSVTRPLTGAKIAVHYGC
                     HLMKPAKERHFGDTENPMWIEELVAALGAEPVQYRNKMQCCGAGGGVRGYDIAHALDI
                     TNEKMINLQEVGADALTEVCPFCQLQYDRGQIEIEEKFGITWGLPILHYNELLGLAQG
                     MSPDELALDLHAVSVEPFLKKIL"
     gene            complement(4663..5244)
                     /gene="hdrC"
                     /locus_tag="PLI76_07935"
     CDS             complement(4663..5244)
                     /gene="hdrC"
                     /locus_tag="PLI76_07935"
                     /EC_number="1.8.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase subunit C"
                     /protein_id="HOI62001.1"
                     /translation="MAVKKDYKDQKLAERLRDRKYYIPDSNPEFIKDVEKIGRTAAHM
                     CYQCGTCTGSCPSAPRSSYRIRLFMRKAILGLEEEALTDPDLWLCTTCYSCTDRCPRD
                     LAPTDAIMAMRNLAFKRDIVPRNFLKTAQLIYQSGHGVPNNAANRVAREKLGLEAEPE
                     TTHKYPEYLPGIRKIMDHYRLKENADRILAEGE"
     gene            complement(5255..6424)
                     /locus_tag="PLI76_07940"
     CDS             complement(5255..6424)
                     /locus_tag="PLI76_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOI62002.1"
                     /translation="MALFPKYSKTREGQNVIMEQKLLKAVNNLILNAETCTGCGICVD
                     ACPEEAIVLGPVGATRRGAIDYAEPVDVNPEKCSYCGVCVIMCPFNAMTLKIDGEERL
                     PILEKEGFPQYDMVTVIDEEKCDRCTICEEVCPRDAINRDVPAFEGGEEAGRPRETAL
                     QTKTTFKVDMEKCSVCGICGELCPSITVVRKPRTPETGKIEGDVLWEESTCDGCKICV
                     EACPEDAITLEREVVSDRLPGKVDIQQDDCCTCTWCVQTCPTEAITVEKIFEGEIEIN
                     PEKCPGGCSTCVEVCPCNALYLPTPLPAKEMKGRIEPNIAINKDFCIFCGACVNACPS
                     EDAIILRRTAIRMQDKDSELFRDIKEKLLTTRTSKVKETTPGEVEIKVLGKVEEA"
     gene            complement(6440..6712)
                     /locus_tag="PLI76_07945"
     CDS             complement(6440..6712)
                     /locus_tag="PLI76_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOI62003.1"
                     /translation="MAFALHINMERCTGCNNCVVACPVNALELHTEEPVTLEKIYKVK
                     DGKAIILDFNSELCAGCGVCVEACPYEVIKLAGPWESRAKARRVEA"
     gene            7492..>8069
                     /locus_tag="PLI76_07950"
     CDS             7492..>8069
                     /locus_tag="PLI76_07950"
                     /EC_number="2.3.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867620.1"
                     /note="catalyzes the transfer of a formyl group from
                     formylmethanofuran to tetrahydromethanopterin
                     tetrahydromethanopterin; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran--tetrahydromethanopterin
                     N-formyltransferase"
                     /protein_id="HOI62004.1"
                     /translation="MELNGVTIDDTYAEAFPTWVARPIITAVTEEWAYRAAVEAVGFA
                     TSTIGCPAEAGIDCFVPADETPDGRPGYAILICASKKKLKEQVVERLAECVLTAPTTA
                     VFDGLADVVAEVQEKLPVKLHFFGDGYEQKREVGGRTVWAIPIMEGEYIGEEEFGAVK
                     GVAGGNFFIMGENQMAALMAAQAAVDAISGVCG"
BASE COUNT         1432 a         2367 c         2448 g         1822 t
ORIGIN      
        1 tcggcatgat ggccgctttc gtgtactcgt tcaagtcacg ggtgaccgcg atggccgcat
       61 cgatgttgtg gttctttccg agtgactggg tcacgcccat accgaagaag atgatcgcga
      121 accggccgct cttgagcata tcggcggcct cgtaaatctt ctccttcggg atgccggcga
      181 cgacatccgg gagcttttcg cccttgaacg ccacgcggaa cgcatcgagg agttcgtagt
      241 cgcgcccctg ctcgatctgg aggtggacgt ccgctatccg ggcggtgtcg gtgggccgcg
      301 ggtcgacgac gatcatcttc cggcccgtgt ggcccttacc ggtgaagaac ccacggggga
      361 agatcgagta ccgggacata tgccgcgggt gggcgtgtgc cgggttgcac ccccagaaga
      421 ggatccggtc ggcacggttc ttgacctcac cgagcgtgca gctcggtatg ccgatatcct
      481 gaacagcgat aagggatgtt ccgtggcaga cggttgccgt gttatccatg accgccccga
      541 ccatctcgcc gatctcggac ccgacggcct gggcctcgca gttcgtggag ctccagccgt
      601 acatcagggg cttctttgca tcggcaagca tccgcgctgt gtactcgatt gcctcgtcgt
      661 aggagatctc cttccaggaa ccatcgtcct gccgcatccg ggggcgggtg agacgatccg
      721 ctgcctggga gtggagaaac ttctcggcgc cgatggcgca ggcgttgtag acctcgaaga
      781 gtttcttccc gtcatcgctg acgacgacct cgaggtcgtc gcagagtgtc ccgcagaacg
      841 ggcagaccac atcggttact gtctttgtca tgccagctca cccctgcatc ctttgcgcac
      901 cagttcaagc gaatcaagga caggttcgtt tttggcgacc tccaccgtga cgggtgtacc
      961 cttaaaagtt ggcatccccg tcgagtaggt gttcggattg atcaccctgt ttgcccatgg
     1021 gcccatcggt atatacgcca caccgggatg gggcccctgg gttgcctcga ctgccttgac
     1081 aacgacgctg ccatactgac tggtgacacg tacggtcgtg ttgcgaaaag ctccgagctt
     1141 cttgaagtcc gccggatcga gctcgatgat cccgcaggcg gcggtgtagg cgggtttttc
     1201 tttccccgcc tccatcgaca ccccctgctg gatggtgcga cccgtgatca aatttactct
     1261 gattgccatt attattcatc ccctgttata ttgtatttat ctggcaagct ctttgagcgg
     1321 gcttgggcca agctcggtga tggtcctgac gacgtcttcc atgacgtgcc cccactttgc
     1381 accctcagat gcggagacga aggtcatcct gagacgcttg tcgtcaagac cgatgttctt
     1441 gaggacactc ttcaagagga acatcctctt tgcagccttg tagttgccct caaggtagtg
     1501 gcagtcgcca aagtggcacc cggagaccag cacgccatct gctccatcct ggaatgcctt
     1561 caggatgaag agggggtcga cacggcccgt gcacatcacg cggacggcac ggatgtccgg
     1621 cgggtactgg atacgggcac cgccggcgag gtctgcaccg gcataggagc accagttgca
     1681 gatgatggcg atgatcttgg gtttccagtt ctcgtctgcc atttactgtt cacctccaag
     1741 gaggaatgca tcgatctgtg caacgatctg cggtgttgca aagtgctgca tccagatagc
     1801 accgccggga cagaatccgc cgcaggtgcc gcagcccttg cacttggcct cggtgacatg
     1861 catgaccgtg cggccgtcct tctcgatgag cgagagggcc tggtaggggc agaggttgac
     1921 acacagtccg catccggcgc acttgtcctc catgcacacg gcgaagtaag gctcgagctc
     1981 cacctcgccg cggtggatcg ggatggatgc cgcggatgcc gcaccctctg cctgggcgac
     2041 cgtgtcgggg atatccttgg gtccctggca gacgccggcg aggtagacac cggcagtggt
     2101 ggtgccgcag gggttcagct tcgggtgagc ctctaacagc cagccgtcct gcgagcagga
     2161 gacaccgaag agtctccgcg tcttctcggt atcggcggag ggctggacgg cggccgcgag
     2221 cacgaccagg tcgacttcca tatcgatggg ccggccaagc agcgtatcct cagcgttgac
     2281 gtggaggttc ttcgtaaccg ggtcctcgag gatgtttgca accctgccgc ggatgaactt
     2341 cgcaccctcg tcctggatgc ggtagtagaa ctcctcgtac atcttgccga aggaccggat
     2401 gtccatgtag aagatgtagg ggatcgcgcc ggggatcttc tcgatgatct ggtgggcgtg
     2461 cttgagcgag tacatgcagc agaaccgcga gcagtacggc ttgccgacgc cggtattgtc
     2521 acgggatcct gcacagagga cgaatgcgat cctctttggg gtctcgccgt cgcttgggcg
     2581 gatgaggtgg ccgccggtcg gtccggacgc gcagatcaac cgctcgaact caaggctcgt
     2641 gatgacgttg tcgtagtgct tgtaacccca ctcgaacttc ttctcgatcg ggaagatatc
     2701 gtatcccagg gcgaggacga cggtgccgac cttgacattc atgagttcgt cctttgcatc
     2761 gagatcgatg gccttcctct cctcgccgca tgcctcgacg cagaggccgc acttgacgca
     2821 ggcgtcgaag tcgacggtgt agatcagggg cgagacctgc gggtggtaga tgtagatcgc
     2881 ctttctcggc gccatgccga gctcgaacgg gttcggcttg atgaccggac agacgtcggt
     2941 acagtcgccg catccggtac agccgccgcc gatgatgccg cgggctgtgg cctcatccgg
     3001 agtgagaacg ccgcgcgcct tcttccggag ggtcacgtcg aagttgccga tgtagccctc
     3061 gaccgcctcg acctcggtgt aggtgaggag ctcgatgcct tcgtggcggt agacatccac
     3121 catcttcggg gtaaggatac actgcgagca gtccagcgtc gggaacgtct tgtcgagctg
     3181 ggacatcttg ccgccgatgg tgggttcctt ctccacgagg taggtcttga tgccggcgct
     3241 cgcaaggtcg agcgcggcct gcatgccgcc gacaccggcg ccgacgacca tcgccgtctt
     3301 ctccacgggg acgctcttcg gtatgagatc ctcaaggagg gatgcctttg ctacggcgat
     3361 cctgactgca tcttttgcct ttgcggtggc ctcttcgggc tggtgcatgt gcacccacga
     3421 gttctgctcg cggatgtttg ccatctcgaa ccggaagggg tttaagccgc cctccttggt
     3481 cgcgttacgg aacgtgggct cgtgcaggcg gggagaacat gctgcaacga cgatcccggt
     3541 cagtttctgc tcgttgatgg cctctgcgat cttgttctgg ccgggggtcg agcacatgta
     3601 ctggtagtca tcggcgacga tgacgttcgg gagcgtcctg gcgtactcca ttactgcctt
     3661 aacgtcgatg gagcccgcaa tattggtacc acagtggcac acaaaaacgc caattcttgg
     3721 ctcttcgtac tttttgactt ctgccatgta tttgcacctc acaggatctt cttcaggaac
     3781 ggctcgacac tgacggcgtg gaggtcgagt gcaagctcat ccgggctcat gccctgcgcc
     3841 agtcccagga gttcgttgta gtgcagtatc ggaagccccc aggtgatgcc gaacttctcc
     3901 tcgatctcga tctgccctct gtcgtactgg agctggcaga acggacagac ttcagtcagc
     3961 gcatcagcac cgacctcctg cagattgatc atcttctcgt tcgtgatatc gagtgcatgc
     4021 gcgatatcgt atccgcggac accgccgccg gcaccacagc actgcatctt gttgcggtac
     4081 tgtaccggtt cggcgccgag agcggcgacg agctcttcta tccacatcgg gttctcggtg
     4141 tcgccgaagt gccgctcttt ggcgggcttc atcaggtggc aaccgtagtg aacggcgatc
     4201 ttcgcgccgg tcagggggcg cgtgacgcta tcggcgagct tcttgatacc cacgatcttc
     4261 gggtcgtagt agagctcggc gaggtgccag acgtcgacgg ttcccttgaa ctccatatcg
     4321 atctctttga gcacctcgtt gacgccatcg cggagttcgt cgttgtgctt gagcttgtgg
     4381 ttgacttccc agatggactt gtagcagccg ttgcagatca gggtgatgtc cgtcttcatc
     4441 tgctcggcaa gcacgacgtt tcgcgcagcc attgcgtacc agacgtttaa gtcgatcgaa
     4501 ccgaacgcgc ccggcgccgg gcagcagctt gcgcccttca ggggaaggag gtcgatgccg
     4561 acattcgcgc tggtcttgat ggccgccgcc tcgatcccgg ggtaccggtt cggtgcgatg
     4621 catccgagga agaatgcgta ctgatgtctg ttgctcatgg tttcactcac cctccgccag
     4681 tatcctgtct gcattctctt tgaggcgata gtgatccata atcttcctga taccgggcag
     4741 atactccgga tacttgtgcg tcgtctcggg ttccgcctca aggccgagct tctctcgtgc
     4801 aacccggttt gcagcgttgt tcgggacgcc gtggcccgac tggtagatca gctgggctgt
     4861 cttgaggaag ttgcgcggca cgatatcccg cttgaacgca aggttcctca tcgccatgat
     4921 cgcgtcggtc ggcgcgaggt cacgggggca ccggtcggtg cagctgtaac aggtggtgca
     4981 gagccagagg tcggggtcgg tgagggcttc ttcctcgagg ccgaggatgg ctttcctcat
     5041 gaacagccgg atacggtacg aactccgggg tgccgagggg cacgagccgg tgcaggtgcc
     5101 gcactggtag cacatgtggg cggccgtccg cccgatcttc tccacatcct tgatgaactc
     5161 ggggttgctg tcgggaatgt agtacttcct gtcccggagt ctctcagcga gtttctggtc
     5221 cttatagtct ttcttcactg ccattgacta tccctcacgc ctcttccact tttcccagga
     5281 ctttgatctc gacttctcca ggagtggtct ctttcacctt cgatgtcctc gtggtgagca
     5341 gtttttcctt gatgtccctg aagagttccg aatccttgtc ctgcatccgg atagcggttc
     5401 gccgcaggat gatagcatct tcgctcgggc aggcgttgac acaggcgccg cagaagatgc
     5461 agaaatcctt gttgatggcg atgttcggtt cgatcctgcc tttcatctcc tttgccggga
     5521 gcggtgtggg caggtagagt gcattgcagg ggcagacctc gacacaggtc gaacaaccgc
     5581 cggggcactt ctcggggttg atctcgatct ctccctcgaa gatcttctcg acggtgattg
     5641 cctcggtggg gcaggtctgg acgcaccagg tgcaggtaca acagtcgtcc tgctggatgt
     5701 cgaccttgcc gggcagtctg tcgctcacga cctcgcgctc aagggtgatt gcgtcctcgg
     5761 ggcaggcctc gacacagatc ttacatccgt cgcaggtgct ctcctcccac aggacatccc
     5821 cttcgatctt gccggtctcg ggggttctgg gcttgcggac gacggtgatg ctcgggcaga
     5881 gttcgccgca gatgccgcag acgctgcact tctccatgtc gactttaaac gtggtcttcg
     5941 tctgcagagc ggtctcacgg ggtctgccgg cctcttcgcc gccctcgaat gcggggacgt
     6001 cgcggttgat ggcgtctctc gggcagacct cctcgcagat ggtacaccgg tcgcacttct
     6061 cttcgtcgat cacggtgacc atatcatact gggggaatcc ttctttctca agtatcggga
     6121 gtcgttcttc tccgtcgatc ttgagcgtca tcgcattgaa ggggcacatg atgacgcaga
     6181 cgccacagta tgaacacttt tcagggttga cgtcgacagg ttcggcgtaa tcgatcgccc
     6241 cgcggcgtgt cgcaccgact ggtccgagca cgattgcctc ttcggggcag gcatcgacac
     6301 agatgccgca gcctgtgcag gtctccgcgt tcaggataag gttgttgacc gctttgagga
     6361 gcttctgctc catgatgacg ttctgtcctt cccgcgtttt ggaatacttt ggaaacagtg
     6421 ccatatttta gtatcactcc taggcttcca ctcttcgggc ctttgcccgg ctctcccacg
     6481 gtcctgcaag tttgataact tcgtaggggc atgcctcgac gcatacgccg caaccggcgc
     6541 agagctcgga gttaaagtca agaataatgg ctttgccgtc cttgaccttg tagatcttct
     6601 ccagggttac cggttcttcg gtatggagct cgagagcgtt gaccggacat gccaccacgc
     6661 agttgttgca gcctgtacat cgttccatat tgatgtgcaa tgcaaatgcc atggttatca
     6721 cgcaccagat ctgatcgatt aaaaatcgat ataaaaagaa ttgtcaaaga atatacataa
     6781 actttctgaa attcggcagt cttatattcc agaactatga ttattcgtca aattttataa
     6841 attaaatcgg ttgttatgtt aacccgatgt cacggatatc ggcgcaaaac gcatctcggg
     6901 gcagaatgtc aaagcgaaaa ccttaaattt taagtcgggc cttctggtga aaattggtgg
     6961 ccaatccggg ccggcagcgt gaagaaccgg ccggatctcc ccgggacccg gcgataaccc
     7021 ccaccttcct ccccggaaga gcccgccgcc cgttgcgcgg tttgggcgac acctgtttac
     7081 gtaatcgttg gttctccacc ctttgcgccg ggcacgtgcg cccacccgga atgcccgtcg
     7141 cgaccctgcg cccacggtcc tccatagcat cccgttcagg cccggtcggg cgggatcgtg
     7201 ggaccgggaa gggcttaacc cggcctccga cgcggatccg ggagatatgg atgttttgca
     7261 ccccaatagc gcaatcaggc tgaatttctg cgttctgtcg tccggcagcc ggttcttcgc
     7321 cggtttaccc tgccgtggcg gtggctgtcg gcgggccggc ccggtctccg ggggggccgc
     7381 gcggttgagg ctgccgatct ccggcgccga tccggtgtgc acgcccggga gaaaaagaaa
     7441 ccttattctg tccgcatcgt gacattgtag caaacgaggt gtattctgtc catggaattg
     7501 aatggcgtta ctatcgacga tacctatgca gaggcgttcc cgacctgggt ggcgcggccc
     7561 atcatcaccg ccgtcaccga ggaatgggca tacagggcgg cagtggaggc cgtcggtttt
     7621 gccacctcca cgataggttg tccggctgag gcgggcatcg actgcttcgt ccctgccgac
     7681 gagactcccg acgggcggcc cggctacgca atcctgatct gtgcgagcaa gaagaagctc
     7741 aaggagcagg tcgtggagag gcttgccgag tgcgtcctca ccgcaccgac gacggcggtc
     7801 ttcgacggcc ttgccgacgt cgttgccgag gtccaggaga aactcccggt caagctccac
     7861 ttcttcggcg acggctacga acagaagcgc gaggtcggcg gcaggacggt ctgggccatc
     7921 ccgatcatgg agggcgagta tatcggcgaa gaagagttcg gtgccgtcaa gggtgtcgcg
     7981 ggcggtaact tcttcatcat gggcgagaat cagatggccg cgctcatggc cgcgcaggcg
     8041 gcggtcgacg ccatcagcgg cgtctgcgg
//
LOCUS       DAOSEE010000098         7980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_3352_length_7980_cov_198.637098, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000098 DAOSEE010000000
VERSION     DAOSEE010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7980
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3352_length_7980_cov_198.637098"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="PLI76_07955"
     CDS             <1..543
                     /locus_tag="PLI76_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HOI62005.1"
                     /translation="LGYRTSRSPLDNTNRHRIYACIRDHPGIAPAEIARATGINRGTV
                     HYHLSCLRDAGLVGILRRKGRGGYFRNGDCNPTEKAMYLHLNNDIRRQILALLLEKPG
                     ITQSEIADIMGIARSTAAWHLQKLAADELVESDRDGRTVHYALTCEALAFSGALSSWG
                     TLFLPGDKTSAGNCADSLPQ"
     gene            926..1708
                     /locus_tag="PLI76_07960"
     CDS             926..1708
                     /locus_tag="PLI76_07960"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HOI62006.1"
                     /translation="MARWSRILLVLIIVMILALPGTATARYVVEPVAEEYAGDWHDLE
                     EITFRDLPLRVMLLHLLFTILPGLPYIGELFYALSLLVPLGFRRVMRHNILDDDLRRD
                     LYRQITANPGIGTTELGELTGASRGRLRYHLDMLIREGKVAAVSYRNRARHFAGNQRY
                     TALEERIFASLRDETPAVILTHLHRSPGATQNEIKDQLGLAAPTISQHMQQFEAEGIV
                     VRDQEGRSFRYRLTGDAREILNEHQKFPTLKSASLRKWRSVS"
     gene            complement(2078..2707)
                     /locus_tag="PLI76_07965"
     CDS             complement(2078..2707)
                     /locus_tag="PLI76_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HOI62007.1"
                     /translation="MKIVVSVEEPGTIDQVLEYNPTFIEVRLDRMEGDLPDLVRAIRE
                     KTAIPLIATLRSREEGGRFVGDADLWMRVVGPVTRYVDYVDVEARYRDHAPFIKSQGV
                     QIVASLHTNEMPTPPELGRIEALLRSYGDIPKIVVKPRTADDLLTLVTFTHQAQKPIC
                     TSIMGAGFRYARAVLPLFGSEFVFCHAGTPTAEGQYHIREMRQLADLLK"
     gene            complement(2710..3540)
                     /locus_tag="PLI76_07970"
     CDS             complement(2710..3540)
                     /locus_tag="PLI76_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HOI62008.1"
                     /translation="MNSTGIIGYGHMGSMLVKGFITSGALRAGEIVVASRSRRSLDAC
                     AAAWPGVGIAATNADIARRCRTVIIAVRPQEVGGVLCEIAPALAGDEHIVSLAAGISL
                     AAIEDAYTGPVTRVVPTITSAVGEGTTLVCHGRQVGMDNALRVERLFSSIGGVVAVSE
                     ADLPAATLLSSCGPGLLASLIEELAGAAVRASGIPPDRALLLATEMVAATGAYLRETG
                     RAPGDLVGEVATGGGITEVGVRGFRERLPAVFDEVFAAMLERYGTVGRPLSDERSYPR
                     "
     gene            3633..6173
                     /locus_tag="PLI76_07975"
     CDS             3633..6173
                     /locus_tag="PLI76_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOI62009.1"
                     /translation="MPHDHPQGTGPVKPPELLAPAGSPEALAAAVAAGADAVYLAGRR
                     FGARHYAANFSDEELRSAIDYAHLHDVRVFVTVNTLVRDAELSDVARYLLRLYEAGTD
                     AVLVQDAGVASLAREIVPDLPLHASTQMTVHNREGVARAAREGFSRVVLAREVTLAEI
                     EDIAEVQGIGIEVFAHGALCYCYSGQCLLSSVIGGRSGNRGMCAQPCRKPYRLVTADK
                     DDYGRPKSLRAVPAEDRYLLSTRDLAVYPHLDRIARAPVASLKIEGRMRSPEYVATVV
                     DIYRRALDTIAAGAPWSPSREDMRDLALAFNREFTEGYILGAPDIMARDRPGNRGVLL
                     GTVSGYDPRRREASVRLAGDTSPRSGDGLAFSSDDPDRGVGAIVRGTPAVKNGMVRIG
                     VGAPVGAGARVFLTKSADLEERAKRIMANPLPQIPIDILVTWEGGTPSLEATVPVCGG
                     EPLRVLYRADIVMEPARSRPLTGEQIAEHLGKTGGTPFTVRKMDLRYPGGLFTPIGEL
                     NRIRRTFLERVGEAVLAARRPGPDAVHAARERVEAAIEGMTRTAGSRRTPRTPSVSVY
                     VDTLEAAAAAAREGARTIYIEPSGPVGLRFLEEAATACREGGADLVWKWPTVTRRGFL
                     DAAAPLLPSLYDAGIRGVMVSGMGAADAVRNAEPRMRLYGAAGLNVWNHRTAGSLSAL
                     FLRCTVSPELPAADAAELAGSVGNTPELEVIVQGTIEAMVTEDRLVAAVAGKEPDDRF
                     LGLEDQRRRIFPLRHDSEGRTYIANAAEICLIDRLPGVAGMGIDTVAIDARGRGACYA
                     GDMVRLYREGIDAVNRGDTGILPALKDEVRRRALGGITGGHFVRGLAE"
     gene            6261..6929
                     /gene="tpiA"
                     /locus_tag="PLI76_07980"
     CDS             6261..6929
                     /gene="tpiA"
                     /locus_tag="PLI76_07980"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOI62010.1"
                     /translation="MVSPFILVNLKTYQEGMGSNAHRIAAAAEAVARESGVAIGIAPV
                     FTEIRPLSQHYAIPVYAQHIDAVTPGAHTGHILPEAVRAAGARGTLINHSERRLTLAD
                     IDACVENARRLHLESVVCTNNDATSAAAAALRPDYVAIEPPELIGSGISVSKADPGII
                     ERSVSAVRAVNPDVKVLTGAGIQSGECVKIAVDLGTCGVLLASSVVKAADPEAVFRDL
                     ISLL"
     gene            complement(6934..7488)
                     /locus_tag="PLI76_07985"
     CDS             complement(6934..7488)
                     /locus_tag="PLI76_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOI62011.1"
                     /translation="MEIPTPAELREKRLRMGLKQADVARMAGISQSMVARIEAGSVDP
                     RVSTLAKIVDVLQAAEHSTVTAATVMTAPVLSVTPDDPVNRAVEIMGQNGISQLPVLE
                     GGVPVGCISESAIMSAMEEGGLHQTHRKLVQDYMEPGFPTVPPIAPIDTIVHLLHHNH
                     AVIVVEQGKVQGVITKHDLISLIT"
     gene            7611..7874
                     /locus_tag="PLI76_07990"
     CDS             7611..7874
                     /locus_tag="PLI76_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62012.1"
                     /translation="MAVNREQQQKVLTKVREILSTYHTRDAVKIELESRGFTIRAEHG
                     DVVSLENTPAEIFVQLFVNDRGDIVDTHVVTFEEIELRPPAKE"
BASE COUNT         1431 a         2676 c         2520 g         1353 t
ORIGIN      
        1 ctcggttacc ggacctcacg cagccctctg gacaacacga accggcaccg gatctacgcc
       61 tgcatccggg accacccggg gatcgcgccc gcggagattg cccgtgccac cgggatcaac
      121 cggggaacgg tccattacca tctctcctgt ctccgggatg ccggcctggt cggtatcctg
      181 cgccgaaaag gcaggggagg ctacttccga aacggcgact gtaatccaac tgagaaggct
      241 atgtacctgc acctcaataa cgatatccgc cgacagatcc tcgccctgct cctcgaaaaa
      301 cccggcatca cgcagtcgga gatagccgat attatgggca tcgcgcgctc cacggctgcc
      361 tggcacctgc agaagctcgc cgcagacgaa ctcgtcgaat cggatcgcga cggtcggaca
      421 gtgcattacg cactcacctg cgaggctcta gccttctcgg gggctctgag cagctggggg
      481 acactcttcc ttcccgggga taaaacgagc gctggaaact gcgctgactc tctccctcag
      541 tagataccgg ggtggatact ctcgttcgca ggggaatgag cgcgatcgac cggttcgcag
      601 gaaaactcgt ttcggggaag catcatttta gtaaacacca cgcagggcga gacggaccgg
      661 tactcgatcg atgttcccgc aggcaaaacc gaactcatcg ccgacctgaa ctggggcgac
      721 tcatccgact cgctccggct gatggttcat gtgccggatg ccgtgctcgg gctgtaattt
      781 gcgcataaag gactccggcg gcctgcaccc cgggatatgg cggttcgagg cctacgggaa
      841 cagagtagcc ggcaccctgg actacacgct tatccggcga taaaagagcg aaacattgaa
      901 catctcttcc atcggaccaa ccaccatggc gcggtggagc cggatacttc tcgtcctcat
      961 catcgtcatg atcctcgcac tcccgggcac ggcaaccgcc cgctacgtgg tcgagccggt
     1021 ggcggaggaa tatgccggcg attggcacga tctcgaagag atcacgttcc gggatctccc
     1081 gctccgggtg atgctcctcc accttctgtt cacaatcctg cccggactcc cctatatagg
     1141 ggaactcttc tacgcgctca gccttcttgt gcctctcggt ttccggcggg tgatgcgcca
     1201 taatatcctc gacgatgacc tcagacgcga tctctaccgg cagatcaccg ctaacccagg
     1261 tatcggaacc acggagctcg gggagctgac gggtgcatca cgagggaggc tccgctacca
     1321 cctcgatatg ctcatcaggg agggcaaggt ggccgccgtg agctaccgaa accgtgccag
     1381 gcacttcgca ggaaaccagc ggtataccgc tctcgaagaa cgcatctttg ccagcctccg
     1441 ggatgaaacg ccggcggtga tactcacaca cctccacagg tcacccgggg caacccagaa
     1501 cgagatcaaa gatcaactcg ggcttgctgc tccgacgatc tcccagcata tgcaacagtt
     1561 cgaggcagag gggatcgtcg tcagagatca ggaggggaga tccttccgct acaggctcac
     1621 cggagatgcc cgggagatcc tcaacgagca ccagaaattc cccaccctga agagcgcatc
     1681 gctgcgtaag tggcgttctg tctcataatg tccagctcct cccgtgagaa ccctgtctta
     1741 tactggccga agatacacca gatcctcctg gcggtccccc ggcgctcctt gctgcacaga
     1801 agcctgctgc cccgaatcga cacccgggat aacatacgac ccggttgcgt tggagatgtt
     1861 cctaatggga agcacctcga tggccgatgt tccggggtgt tgcggcggcg ggcctgcggc
     1921 gagccctcca gccgtcgccc cgagaccgga aggagcaacg agatgacgaa gaagatcacg
     1981 agggtgaaga tcaatgcgtt gccgctctcc cggacggccg tcgagaatgc cagggcaacg
     2041 gcaaaccagg caaacaggaa gaggaacggg gccccgctca cttcagcaga tccgcaagtt
     2101 gccgcatctc ccggatgtgg tactgccctt ccgccgtcgg ggtgccggca tgacagaaga
     2161 cgaactccga cccgaagagc ggcaggacgg cccgggcgta gcggaacccg gcacccatga
     2221 tgctcgtgca gatgggtttc tgagcctggt gggtgaacgt gacgagcgtc aggaggtcgt
     2281 ctgcagtgcg gggcttcacg acgatcttcg gtatgtcgcc gtatgacctg agcagggcct
     2341 cgatcctgcc gagttccggg ggggtgggca tctcgttcgt gtggagggat gcaacgatct
     2401 ggacaccctg gctcttgatg aacggcgcgt ggtcccggta acgggcctcc acgtccacgt
     2461 agtcgacgta gcgcgttacc ggcccgacga cccgcatcca gaggtccgca tccccgacaa
     2521 accttccccc ctcctcccgg ctcctgagcg tggcgatcag gggaatggcg gtcttctccc
     2581 ggatcgcacg gaccaggtcc gggaggtcgc cctccatccg gtcgagcctg acctcgatga
     2641 acgtcgggtt gtactccagc acctggtcga tggtgccggg ctcctccaca gagacgacga
     2701 tcttcatact tatcgggggt atgaccgctc atcgcttaaa ggtctgccca ctgtaccgta
     2761 gcgctccagc atcgcggcga agacctcgtc aaagaccgcc ggcaaccgtt cccggaaccc
     2821 ccgcacgccg acctcggtga tgccgccgcc ggttgcaacc tcccctacca ggtcccccgg
     2881 cgccctgccg gtctcccgga ggtatgcacc cgtggccgcg accatctccg tcgcgagcag
     2941 aagagcccgg tcgggcggaa tcccgctcgc ccgaaccgcc gcgcccgcca gctcctcgat
     3001 gagggaagcc agcagtcccg gcccgcagct cgagagcagc gtcgccgccg gaagatcggc
     3061 ctcactgacg gccaccacgc ctccgatgga tgagaagagc ctctccaccc ggagagcgtt
     3121 gtccatcccg acctgccgcc cgtgacagac cagcgtcgtc ccctctccga ccgcggaggt
     3181 gatcgtgggc acgacccggg tgaccgggcc cgtatacgca tcctcgatcg ccgcaaggct
     3241 gatgccggcg gcaagcgaga cgatatgctc gtcgccggcc aacgccgggg cgatctcgca
     3301 gaggacgccg cccacctcct gcggcctgac ggcgatgatg accgtccggc accgccgggc
     3361 aatgtccgcg ttcgttgcgg cgatgccgac cccgggccac gctgcggcgc aggcatcaag
     3421 gctccgccgg ctccgggacg cgaccacgat ctcgccggcg cggagtgccc cggacgtgat
     3481 aaaccccttc accagcatgc tccccatgtg gccgtagccg ataatccctg tgctgttcat
     3541 cctttccctt cacatcggcc tctcgcgcaa aaagaatact catcctcacc ggggtggagt
     3601 atataggccg gcacggcgaa cggtacccga caatgccgca cgatcaccct caggggaccg
     3661 gcccggttaa gcccccggag ctgcttgccc ccgccggatc gccggaagcc ctggctgccg
     3721 cggtcgccgc cggcgcggat gccgtctacc ttgccgggag gcgtttcggg gcccggcact
     3781 acgcggcgaa cttctccgac gaagagctcc ggtccgccat cgattacgca cacctccatg
     3841 acgtgcgagt tttcgtcacc gtaaacaccc tcgtccggga tgccgaactc tccgacgtgg
     3901 cccggtacct cctccggctc tacgaggccg ggaccgacgc cgtcctggtg caggatgccg
     3961 gggtggcgtc gcttgcccgg gagatcgtcc cggaccttcc tctccacgcc tccacccaga
     4021 tgacggtcca caaccgcgag ggcgtggccc gggccgcgcg ggaggggttc tcccgcgtgg
     4081 tgctggcgcg ggaggtcacg ctcgccgaga tcgaggatat cgcagaggtg caggggatcg
     4141 ggatcgaggt cttcgcccac ggtgctctct gctactgcta ctcggggcag tgcctcctct
     4201 cctcggtcat cggagggcgg agcgggaacc gggggatgtg cgcccagccg tgcaggaaac
     4261 cctaccggct cgtgaccgcc gataaggacg attacggtcg cccgaagtcc ctccgggcgg
     4321 tgcctgcaga ggatcgctac ctgctctcga cccgggacct cgcggtatac ccgcacctcg
     4381 accgcatcgc acgtgcaccg gtggcgtcgc tcaagataga ggggaggatg cgctccccgg
     4441 agtacgtcgc cacggtggtg gatatctacc ggcgcgccct cgacacgatc gccgccgggg
     4501 ctccctggtc accctcgcgg gaggatatgc gggacctcgc cctcgcgttc aaccgggagt
     4561 tcacggaggg ctacatactc ggcgctcccg atatcatggc ccgcgaccgg cccgggaacc
     4621 ggggcgtcct ccttggcacg gtcagcggct acgacccgcg gaggcgggag gcgagcgtgc
     4681 gcctcgccgg cgatacctcg ccccgctctg gcgacgggct cgccttctct tccgacgacc
     4741 ccgaccgcgg cgtgggggcg atcgtccgcg gcacccccgc cgtgaagaac ggcatggtcc
     4801 gcatcggtgt cggcgccccc gtcggggcgg gtgcccgtgt cttcctgacg aagagcgccg
     4861 acctcgagga gcgggcaaag cggatcatgg caaaccccct gccgcagatt cccatcgata
     4921 tcctggtcac ctgggagggc ggcacgccct ctctggaggc gacggttccc gtgtgcggcg
     4981 gcgaaccgct ccgggtgctc taccgtgccg atatcgtgat ggagcctgca cggagccggc
     5041 cgcttaccgg ggagcagatc gcagaacacc tcgggaagac cggggggacg ccgtttaccg
     5101 tccggaagat ggacctccgg taccccgggg gactctttac accgataggg gagttgaacc
     5161 gcatccggcg aacattctta gagagagtcg gggaggccgt gctcgccgca cggcgcccgg
     5221 ggccggatgc ggtgcatgca gcccgggagc gggtcgaggc agcgatcgaa gggatgacac
     5281 gcacggcagg atcgcgccgc acaccccgga ccccatcggt ctcggtctac gtcgataccc
     5341 tcgaggctgc agcggccgcc gcacgggagg gcgcccgcac gatctacatc gaaccttccg
     5401 gacccgtggg cctccgcttc ctcgaagagg cggccaccgc ctgccgggag gggggcgccg
     5461 atctggtctg gaagtggccg acggtcaccc ggcgcgggtt cctcgatgcg gcggcccctc
     5521 tcctcccgtc gctctacgat gcgggcatcc gcggggtgat ggtgagcggg atgggggccg
     5581 cggatgcggt ccggaacgcc gaaccccgga tgcgcctcta cggcgccgcc gggctgaacg
     5641 tctggaacca ccgcactgcc ggttccctgt cggcgctctt cctgcggtgc acggtctccc
     5701 ccgaactccc ggccgccgat gctgcagaac tcgcggggtc ggtcgggaac accccggaac
     5761 tcgaggtgat cgtgcagggc accatcgagg cgatggtgac cgaggaccgg ctggtcgcag
     5821 cggtcgccgg caaagaaccc gacgaccgtt tcctggggct cgaggaccag cgcaggcgca
     5881 tcttccccct caggcacgat agcgaagggc ggacctatat cgcgaatgcc gccgagatct
     5941 gcctgatcga tcgccttccc ggggtggccg ggatgggcat cgataccgtc gccatcgatg
     6001 cgcgggggcg gggagcctgt tatgccgggg atatggtccg gctctaccgg gaggggatcg
     6061 atgccgtgaa caggggcgac accggaatcc tccccgcgct caaagatgag gtgagacggc
     6121 gggcgctcgg cggcatcacc ggcgggcact tcgtccgggg gctcgcggag tgaatctccg
     6181 gggccgcccg ccgattctcc ctctgccccg gcaccagagt aaatatcagg aggagcaacc
     6241 tattggcaga tgtgatggat atggtctcac ccttcattct tgtcaacctc aagacctacc
     6301 aggaaggtat gggcagcaac gcccaccgga tcgccgctgc ggccgaggcc gtggcaaggg
     6361 agagcggggt cgccatcggc attgcgccgg tcttcaccga gattcgcccg ctgagccagc
     6421 attatgcgat accggtttac gcccagcata tcgatgcagt cacccccggc gcccacaccg
     6481 gtcacatact ccctgaagcg gtcagggccg cgggcgcacg cggcaccctg atcaaccact
     6541 ccgagcgccg cctgactctc gccgatatcg acgcctgcgt cgagaacgcc cggaggctcc
     6601 acctggaatc ggtcgtctgc acgaacaacg acgcgaccag cgccgcagcc gcggccctcc
     6661 ggcccgacta cgtggcgatc gaacccccgg agctgatcgg gagcgggatc tcggtctcga
     6721 aggccgaccc ggggatcatc gagcgatccg tcagcgctgt cagggcggtg aacccggatg
     6781 tgaaagtcct gaccggggcg ggcatccagt cgggcgaatg cgtgaagatt gccgtcgacc
     6841 tcgggacctg cggcgtcctc ctcgcgtcga gcgtggtcaa ggccgccgac cccgaagcgg
     6901 tcttcaggga cctgatctcc ctgctctgat gccctacgtg atcagcgata tcaggtcgtg
     6961 cttggtgatg acaccctgca ccttcccctg ctcgaccacg atgaccgcgt ggttgtggtg
     7021 caggagatgg acgatcgtat cgatgggcgc tatcggggga acggtcggga aacccggctc
     7081 catgtagtcc tgcaccagct tccggtgcgt ctggtggagc cctccctcct ccatggcgct
     7141 catgatggcc gattcggata tgcatcctac cggcacccca ccctcgagca ccggcagctg
     7201 ggagatgccg ttctggccca tgatctcgac ggcacggttg acagggtcgt caggagtaac
     7261 ggagagcacg ggtgcggtca tgacggtggc cgccgtgacc gtcgagtgct ccgccgcctg
     7321 caggacatcc acgatctttg caagcgtgct caccctcggg tcgacgctgc ccgcctcgat
     7381 ccgcgcgacc atggattggc tgatccccgc cattcgggct acgtctgcct gttttaagcc
     7441 catacgaagc cttttttccc gcagttcagc cggggtgggt atctccatat tattactata
     7501 ggtaattttt ttcacttata cgtttgcccg ccggtgcggt catcggcggt atcgcgatcc
     7561 gtcgcccctt cccgggcagg ttgatatagg gcagggttcc actggatggt atggccgtaa
     7621 acagggagca acagcagaag gtcctcacga aggtccgcga gatcctctcg acctatcaca
     7681 cccgcgatgc cgtcaagatc gagctggagt ctcgtgggtt cactatcagg gcagagcacg
     7741 gagatgtcgt gtccctggag aacacacccg ccgagatctt cgtccagctc ttcgtgaatg
     7801 accggggcga tatcgtcgat acgcacgtcg tgacgtttga ggagatcgag ctcaggccgc
     7861 cggctaaaga ataaactgcg ctctaccgag aagagttgca aggagtggga ggagtaagcc
     7921 tccttctgtt ctgtgcggca ttcagctacg cgccatacct gggcggatac cagacgatcc
//
LOCUS       DAOSEE010000099         7963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_3361_length_7963_cov_230.435761, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000099 DAOSEE010000000
VERSION     DAOSEE010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7963
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3361_length_7963_cov_230.435761"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..663
                     /locus_tag="PLI76_07995"
     CDS             238..663
                     /locus_tag="PLI76_07995"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOI62013.1"
                     /translation="MKPQNLSVEEKRRLSGLIVASLSQREEVLFAYVYGSFPRGAFRD
                     IDIAVFLADGSNGIADPIRYETALEQELEDIAGVPIDVRVLNAAPLPFAYSVLKSGDL
                     LLSRDERARCDFACRTYTHYHDFAYYRKRYRREALGLLR"
     gene            complement(822..1754)
                     /locus_tag="PLI76_08000"
     CDS             complement(822..1754)
                     /locus_tag="PLI76_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOI62014.1"
                     /translation="MSGTVMDLLVVADQILILVLLIAVGYAAVSTKIMDPRATRGLSA
                     LLVNVTIPALIIASMQVPFTPERLAGAEILLLATGVLFAFSFALAWAVSKAMQVPPAE
                     EGVLQFAIVFGNVGFMGFPVALTLFGEESLFYVAIFNLVFNILVFSVGIAMLTGGRGK
                     EFDPKLLANPGIAAAIVGFALFLGSIEIPGPFIDSIDLLGGVTTPLAMIIVGAMLATF
                     PAREMVGNWRIWVASAILLLAVPVAYCCLFAPVFADPFINGVMITMAAMPAAANTVIF
                     AEQYGANSRLASQIVFVSTIGSLVTIPLVATVLL"
     gene            complement(1912..2274)
                     /locus_tag="PLI76_08005"
     CDS             complement(1912..2274)
                     /locus_tag="PLI76_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62015.1"
                     /translation="MRPKLKRIEGERKTFTGTFARFGKKDRYRPKKVGDEWVTHDLTV
                     LLVDVRDSDGNPVTDHLWFNYTKGFASLGDLQEGDVIRFDARVRPYIKGYVNQREYID
                     EREVDYKLAYPTKVARAR"
     gene            complement(2387..3304)
                     /locus_tag="PLI76_08010"
     CDS             complement(2387..3304)
                     /locus_tag="PLI76_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOI62016.1"
                     /translation="MVTEFLVVADQIFILVLLIAAGYVAVSTKIMDPGATRGLSALLF
                     NITIPALIVSAMQVPFTSERLADTGTMLLATGAFFALSFVVARIASRAMAVAPQKRGV
                     FEFAIVFGSVSFMGFPVARALLGTGSLFYVALFNIVFNILLFSAGIAMLAGGKGFDPR
                     LLANPGIVATIAGFLLFLASVEIPSPFIDAIDLLGGVTTPIAMIIVGAMLATLPAREM
                     IGNWRVWATSAVILIALPVAYCCLLSTIFPDPLINGTMIMMAAMPAAAGTPVFAEQCG
                     ADARLASQIVFVSTIGSLVTIPLVATVLL"
     gene            3417..3989
                     /locus_tag="PLI76_08015"
     CDS             3417..3989
                     /locus_tag="PLI76_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOI62017.1"
                     /translation="MPEKPFYLAVSALIRDGAGRILLLKRAADDPVNPGKWDLPSGTL
                     DPGETFDCALRREETGMRVILRHVAGELDLATKRIACLMAMPIGPRSPSARSTRITPG
                     SRQGRRRRWTLRSSSGGCFRVENEFSSRPGRVEALRPFSSADRGSAGSPWRRTTLTPP
                     QKVHYAGKGSGSPGLGDRSHMRLPPDHPPD"
     gene            4055..4480
                     /locus_tag="PLI76_08020"
     CDS             4055..4480
                     /locus_tag="PLI76_08020"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOI62018.1"
                     /translation="MSNPEKLSPLARHIIFVQVQIIRKSDLPEDQGAAGIVRDYGVFG
                     SVDFFLEYEARFDTLVDSVKRAAWILHAIAVRHPFQNGNKRTAFVVADAILREEWGFH
                     IGASQKKKGNFLIEVADYQHDVDDVERWIWKYLEKTDEI"
     gene            complement(4775..4849)
                     /locus_tag="PLI76_08025"
     tRNA            complement(4775..4849)
                     /locus_tag="PLI76_08025"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4812..4814),aa:Ile,seq:cat)
     gene            complement(4979..6325)
                     /locus_tag="PLI76_08030"
     CDS             complement(4979..6325)
                     /locus_tag="PLI76_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOI62019.1"
                     /translation="MPGIFRAYDIRGRYPDELDEAMARRIGNAFARLLSAEQVVVGRD
                     MRLSAESLAGAFAKGVLEAGADVADVGEVSTPLLNYAIIAGGFDGGAMVTASHLPGDM
                     NGVKLARKDAVPLSGDRGLPRLEAMVGEEPVARAPGSSRRVDMLDSYIGKVAGFVRNP
                     RPLTIAVDAGNGMAGPEVPRLFERVPAWRLVPMYIVPDGRFPHHRANPLDPETTRDLQ
                     ERVVREGADFGAAFDGDADRCGFIDERGERVREDLVTGLIADYLLGEVPGATILYDLR
                     SSRAVVEAIERAGGRAVRSRVGHAFIKARMREEGALFAGELSGHYYYRDMGFTDNGLV
                     TMVMVANILAASGKTLSALMKPLDRYPTTGEINLRASDPERVFTALAARYRDADLDHL
                     DGLTVGYPAWWFNIRSSHTEPVVRLNLEADTEGLMHEKAREVLGVIREADPGAREV"
     gene            complement(6325..7491)
                     /gene="nifS"
                     /locus_tag="PLI76_08035"
     CDS             complement(6325..7491)
                     /gene="nifS"
                     /locus_tag="PLI76_08035"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HOI62020.1"
                     /translation="MERRRSVYMDYAATTPVRPEVLEAMAPYFSERFGNPSSLYALAR
                     EAKEAVEEARGRVAAAIGADPGEVFFTAGGTEADNWAIKGTALRGKGDHIVTSAIEHH
                     AVIHPCRALEKQGYRVTYLPVDEFGRVDPADAEDAIADETILVSVMAANNEIGTIQPI
                     RAIADIAHDHGVPFHTDAVQAIGAYPVDVDDMGADLLALSAHKFGGPKGAGALYIRRG
                     TRVGTFMDGGAQERGRRAGTENVPGIVGLGRAIEVATADIEGHSRRLVAMRDRLIRGI
                     LDDIPDTRLNGHPVERLANNVSVAFRYVEGESILLLLDALGIAASTGSACTSASLEPS
                     HVLTACGLPHEEAHGSLRLTLGSRNTEEDVDYVLSVLPGVIGRLRDISPLRGEA"
     gene            complement(7497..>7963)
                     /locus_tag="PLI76_08040"
     CDS             complement(7497..>7963)
                     /locus_tag="PLI76_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent protease"
                     /protein_id="HOI62021.1"
                     /translation="SYEGIEGDSASSTELYALLSALSGLPLKQYLAVTGSVNQKGEVQ
                     AIGGVNEKLEGFFEICKAKGLDGTQGALIPASNVQNLMLKEEIVEAARAGKFRIYPVR
                     TIDEGIEVLTGVPAGTRREDGTYEEGTVNYLVDRRLRDMAETMRGFQPTAAK"
BASE COUNT         1477 a         2532 c         2493 g         1461 t
ORIGIN      
        1 cgaccttcat ccacgttgtg ttgaagcccg ggaacgcggc ggtcccgaat attcccctct
       61 ctccggccgg gatccgggag cgatttatgc gggcgatccg ggggtgatta cccctccggt
      121 ctccccgatg ggataccccg ggtcgtcatg tcctgatccc gcaccggcca cggcgaggag
      181 aaccgctcca cccgccagaa cgcaaccctc atcatcaccg gcgccaatca tatcgtgatg
      241 aaacctcaga acctctccgt cgaagagaag cggcggctct ccggcctgat cgttgcctcg
      301 ctctcacaga gggaggaggt tctctttgcc tacgtgtatg ggtcgtttcc ccggggtgca
      361 ttccgcgaca tcgatatcgc cgtctttctc gcagacggca gcaacgggat tgccgatccg
      421 atcaggtatg agactgcgct cgaacaggaa ctggaggata ttgcaggagt acctatcgac
      481 gtcagggtct tgaacgctgc tccccttcca ttcgcgtact cggtcttgaa gtccggagat
      541 ctgctcctct cccgtgatga gcgggcacgc tgcgacttcg cgtgcagaac ctatacccat
      601 tatcacgact tcgcctacta tcgaaaacgt taccggaggg aagcacttgg tctcctacga
      661 tgacgaaaaa gtcaccgcac tctcattgga actggtgcag gcgtgcaggc gcttgagaga
      721 cctcgcccgg atgtaaaaag aggtcatcat cggggatccc catctgattg ccagcgcaaa
      781 gtaccatctc atcgtcggta tcgaggcggc tatcgctctc tctacagcag caccgtcgcg
      841 acgagcggga tggtaacgag cgatccgatc gtcgagacga agacgatctg cgacgcgagc
      901 cttgaattcg cgccgtactg ctccgcgaat atgacggtgt ttgcagcggc cggcatcgcg
      961 gccatcgtga tcatgacacc gttgatgaag gggtcggcaa agaccggggc gaagaggcag
     1021 cagtaggcca ccgggacggc gaggaggagg atggcgctcg cgacccagat ccgccagttc
     1081 ccgaccatct cccgggccgg gaacgtcgcg agcatcgccc cgacgatgat catggcaagc
     1141 ggcgtcgtca cgccgccgag gagatcgatc gagtcgatga aggggcccgg tatctcgatc
     1201 gatccgagga agagggcgaa cccgacgatt gcggccgcga taccggggtt tgcgagcagt
     1261 tttggatcaa attccttccc cctccctccg gtcagcatcg cgatcccgac cgagaagaca
     1321 aggatgttga agacgaggtt gaatatcgca acgtaaaaga gcgactcctc cccgaagagt
     1381 gtcagggcga ccgggaaccc catgaacccg acgttgccga agacgatcgc gaactggaga
     1441 accccttcct ctgcgggcgg gacctgcatg gccttcgaga cggcccaggc aagggcgaac
     1501 gagaaggcga agaggacccc ggtcgcgagg aggaggatct ccgcaccggc gaggcgctcg
     1561 ggggtgaaag ggacctgcat cgaggcaatg atcagcgccg gtatggtgac gttgacgagg
     1621 agggcggaga gcccccgggt ggcccggggg tccatgatct ttgtggagac ggcggcgtaa
     1681 ccgacggcga tcagcagcac caggatgagg atctggtcgg cgacgaccag gagatccata
     1741 accgtcccgc tcatgggtgg ggcactccct cttgctccac cagtgtgggt gccgctactc
     1801 ctttacggtt ctgctcgggg tcggaggtat ggcgagcgag cctgtccggg aggcgctcta
     1861 ccggagttat cgcgcgatca tcatcctgcg gggcgcctcg cctgccggca ctcaccgggc
     1921 ccgggcaacc tttgtcgggt aggccagttt gtagtcgacc tcccgctcgt cgatatattc
     1981 cctctggttg acgtagccct tgatgtaagg cctgactctg gcgtcgaacc tgatcacgtc
     2041 cccttcctgc aggtctccga ggcttgcaaa ccccttcgtg tagttgaacc agagatgatc
     2101 ggtgaccggg ttgccgtcgc tgtcccggac atcgacgagc aacaccgtca ggtcgtgggt
     2161 gacccactca tccccgacct tctttggcct gtaacggtct ttcttcccga accgtgcaaa
     2221 cgttccggtg aatgtcttcc gttccccctc gatccgtttc agcttcggtc tcacgatctc
     2281 actcttcccg gcagactcat acgatcagtg gctgcacctt tccgccatgt tcaatcggag
     2341 cgatatacca taacggttat gcaagcgggg tgaaagtgta ttccccctac agcagcaccg
     2401 tcgcgacgag cgggatcgta acgagcgatc cgatcgtcga gacgaagacg atctgtgacg
     2461 caagccgggc gtcggcgccg cattgctccg caaagacggg ggtgcctgca gcggccggca
     2521 tcgcggccat catgatcatg gtgccgttga tgagagggtc gggaaagatt gtggagagga
     2581 ggcagcagta ggcaaccggt agagcgatga gaatgacggc acttgtcgcc cagacccgcc
     2641 agttcccgat catctcccgg gcggggagcg tcgcgagcat cgccccgacg atgatcatgg
     2701 ctatgggtgt cgtcacgccg ccgaggaggt cgatcgcgtc gatgaagggc gaggggatct
     2761 cgaccgatgc gaggaagagg aggaacccgg cgattgtagc cacgatgccg ggatttgcga
     2821 gcagtcgcgg gtcaaatccc ttccctccgg cgagcatcgc aattccggcc gagaagagga
     2881 ggatgttgaa gacgatgttg aagagggcga cataaaagag ggaaccagtg cccaggagcg
     2941 cccgggcgac cggaaacccc atgaaactca cgctcccgaa gacgatcgcg aactcaaaaa
     3001 ctcctctctt ctggggcgct accgccatcg ctcgcgatgc tatccgggcg acgacgaacg
     3061 agagggcgaa gaaggctccg gttgcgagga gcatcgtccc ggtatccgcg aggcgctcgg
     3121 aggtgaaggg cacctgcatg gcggagacga tcagcgccgg tatggtgatg ttgaagagga
     3181 gggcggagag cccccgggtg gccccggggt ccatgatctt tgtcgagacg gcaacgtagc
     3241 cggcagcgat cagcagcacc aggatgaaga tctggtcggc gacgaccaga aattccgtga
     3301 ccataccgct catgaacggg ctccaccctt ccggatacag cagtgtgggt tctgataacc
     3361 cattacggtt ctgccgtggg ggagattttt ctctcggcag gactctatct ccctgtatgc
     3421 cggaaaaacc gttctacctc gccgtttcgg cgctcatccg ggacggtgcc gggaggatcc
     3481 tcctcctgaa acgcgccgcc gacgaccccg tcaatcccgg gaagtgggac cttcccagtg
     3541 gaacactcga ccccggcgag acgttcgatt gcgccctccg gcgggaggag accgggatgc
     3601 gggtcatcct ccgtcacgtc gccggcgagc tcgaccttgc gacgaagcgg atcgcttgcc
     3661 tgatggcgat gccgataggg ccgagatccc cctcagcccg gagcacgagg attacgcccg
     3721 ggtcgcgcca ggggaggcgg cggcgatgga ccttgcggag cagttccggg ggctgtttcc
     3781 gggtggagaa tgagttttcc agcaggccgg ggcgggtgga ggctttacgg ccgttttcca
     3841 gcgccgacag aggttcggcc ggttcgccct ggcgtagaac gaccttgact cctcctcaga
     3901 aggttcatta cgctggtaag ggctccggca gtcctgggct gggcgataga tcgcatatgc
     3961 ggttgccgcc ggatcatccg ccggattgat gtgatcacgg cgtcggtgtg tggaagcata
     4021 taatcgttct ccaacgaata gaactcaata tatcatgagc aacccggaaa aactcagccc
     4081 tctcgcgcgg cacatcattt ttgtgcaggt gcagataatt cgcaaaagcg atctaccgga
     4141 ggatcaaggt gccgcgggga ttgtgaggga ctacggggta tttggctcgg ttgatttttt
     4201 cttagagtac gaagcaagat ttgataccct ggtcgattcg gtgaagcgcg cagcatggat
     4261 actccatgcg atagcagttc ggcatccgtt ccagaatgga aataagcgca ctgcctttgt
     4321 tgttgcagat gcgatcctcc gagaagagtg gggtttccac atcggtgcct cgcagaagaa
     4381 aaaagggaac ttcttgatcg aggtcgcgga ttatcagcac gatgttgatg atgttgagcg
     4441 atggatctgg aaataccttg agaagaccga tgagatctaa cgttctgcca tgatcatgag
     4501 ggcttcgcgg ttctcctcga tagagtcggt gattgctttc cgctcggatt gcgtaagagt
     4561 ctcgttcata aatgcgcctc tccgccgctg cacagcagga ggattacagg ggactgcatc
     4621 gccgtccgcg ccgggaacca tcactatcac cgaccttgta gtaatacttc atagatgagt
     4681 atgcacgccg gcataaattg atgtcgcata ctcaccggat ctgtgaatgc tctttatcga
     4741 gggtcagaat aacgaccgtt tcaagaactg ggagtgggcc cggagggatt cgaacccacg
     4801 accgcccggt tatgagccgg gcgctctaac cggactaagc tacgggcccg gagatgcgcg
     4861 tcacgattca cggataccag gaacggcgcg ggtttcggca gttcccgaca ttcatgccga
     4921 tcacggtgca tataaatatc cctccttgat cgataaatac gttgcgcgct tcccggccct
     4981 acacctcccg cgccccgggg tcggcctccc ggatgacccc gaggacctcc cgcgccttct
     5041 cgtgcatcag cccctccgta tccgcttcga ggttcagccg caccaccggc tcggtgtggg
     5101 aggagcggat gttgaaccac cacgccggat accccaccgt cagcccgtcg agatggtcga
     5161 gatccgcatc ccggtagcgc gccgcaagcg cggtaaacac ccgttccggg tcgctcgccc
     5221 ggaggttgat ctctccggtc gtggggtagc ggtcgagcgg tttcatgagc gcggagagcg
     5281 tcttgccgct cgcggccagg atgtttgcga ccatgaccat cgtcacgagc ccgttgtccg
     5341 tgaaccccat gtcccggtag tagtagtggc cggagagctc cccggcaaag agggcgccct
     5401 cctctcgcat ccgggccttg atgaaggcgt ggcccacccg tgagcggacg gcccgtcccc
     5461 cggcccgttc tatcgcctcg acgaccgccc ggctcgaccg caggtcgtag aggatcgtcg
     5521 cccccggcac ctcgccgagg aggtagtccg cgatgagccc ggtcaccagg tcctctctga
     5581 cacgctcccc ccgctcgtcg atgaacccgc accggtcggc gtcgccgtcg aacgccgccc
     5641 cgaagtccgc accctcgcgg accacccgtt cctggagatc ccgggtggtc tcggggtcga
     5701 gcgggtttgc gcggtggtga ggaaaccgcc cgtcgggcac gatatacatc ggcacgaggc
     5761 gccacgccgg cacccgctcg aagagacggg gcacctccgg cccggccatg ccgttccctg
     5821 catccacggc gatcgtgagc ggccgggggt tcctgacaaa tccggccacc ttcccgatgt
     5881 aggagtcaag catatccacc cggcgagaag accccggggc ccgggcgacc ggctcctccc
     5941 cgaccatcgc ctcgaggcgc ggcagccccc ggtccccgga gaggggcacc gcgtcttttc
     6001 gcgcaagttt gacaccgttc atatcccccg ggagatggga ggccgtcacc atcgcgccgc
     6061 cgtcaaaccc cccggcgatg atggcgtagt tgaggagcgg cgtgctcacc tcgccgacgt
     6121 cggcgacatc cgccccggcc tccagcacgc cttttgcgaa cgcaccggca agcgactccg
     6181 ccgagagccg catatcccgc ccgacgacga cctgctccgc ggagaggaga cgggcaaaag
     6241 cgttcccgat ccgccgcgcc atcgcctcat cgagctcgtc cgggtagcgc ccccggatat
     6301 cgtaggcccg gaatatgccg ggcatcacgc ctccccccga agcggcgata tatcccggag
     6361 ccgcccgatc accccgggca ggaccgagag gacgtaatcg acatcctcct cggtgttccg
     6421 cgaaccgagg gtgagccgga gcgaaccgtg cgcctcctcg tgcgggaggc cgcaggccgt
     6481 gaggacgtgg gagggttcaa gcgaggccga tgtgcaggcg ctcccggttg aggcggcaat
     6541 accgagagcg tcgagcagga ggaggatcga ctccccctcg acgtagcgga acgcgacgct
     6601 gacgttgttt gcgagccgtt cgaccgggtg gccgttcagc cgggtatccg ggatatcatc
     6661 gaggatcccc cgtatcagcc ggtcccgcat cgcgacgaga cggcggctgt gcccctcgat
     6721 atcggctgtc gcaacctcga tcgcgcgccc gagccccacg atgccgggca cgttctcggt
     6781 cccggcccgg cggccccgtt cctgtgcccc gccgtccatg aacgttccaa cccgggtccc
     6841 tcgccggatg tagagcgccc ccgccccttt cgggccgccg aacttgtggg cggagagggc
     6901 gaggagatcg gctcccatgt cgtccacatc caccggatag gccccgatcg cctggacagc
     6961 gtcggtatgg aacgggacgc cgtggtcgtg cgcgatatcc gcgatggcgc ggatcggttg
     7021 aatcgtcccg atctcgttgt ttgcggccat caccgagacg aggatcgtct catcggcgat
     7081 cgcgtcctcg gcgtcggcgg ggtccacccg gccgaactcg tcgacgggca ggtaggtgac
     7141 ccggtaaccc tgcttctcga gcgcccggca ggggtggatg accgcgtggt gctcgattgc
     7201 tgaggtgacg atgtggtcgc ccttcccccg gagagccgtc cccttgatcg cccagttgtc
     7261 ggcctccgtc ccgccggcgg tgaagaagac ctccccgggg tctgcgccga tcgccgctgc
     7321 cacccgtccc cgcgcctcct ccaccgcctc tttcgcctca cgggcgaggg catagagcga
     7381 agaagggttc ccgaaccgct ccgagaagta cggggccatc gcctcgagga cctccggccg
     7441 caccggcgtc gttgctgcat agtccatgta gaccgatcgc ctccgctcca taccgttcac
     7501 ttcgccgccg tcggctggaa cccgcgcatc gtctccgcca tatcacggag gcgccggtcc
     7561 acgaggtagt tcaccgtccc ttcttcatac gtcccgtctt cgcggcgcgt gcctgccgga
     7621 acgcccgtga ggacctcgat cccctcgtcg atcgtccgca ccgggtagat ccggaacttc
     7681 ccggcacgcg ccgcctcgac gatctcctcc ttcaacatca ggttctggac gttgcttgcc
     7741 ggtatcaggg ctccctgggt gccgtcgagg ccctttgcct tacagatctc gaagaaccct
     7801 tccagtttct cgttcacgcc cccgatcgcc tggacctctc ctttctggtt caccgagccc
     7861 gtgacggcga ggtactgttt cagggggagg cccgagagcg ccgagagaag ggcgtagagc
     7921 tcggtgctcg acgcggagtc gccctcgatc ccctcgtaac tct
//
LOCUS       DAOSEE010000100         7905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_3381_length_7905_cov_215.574395, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000100 DAOSEE010000000
VERSION     DAOSEE010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7905
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3381_length_7905_cov_215.574395"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2482
                     /locus_tag="PLI76_08045"
     CDS             <1..2482
                     /locus_tag="PLI76_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62022.1"
                     /translation="FSKQVTWPVFEPITLTVEATDAAGNYNSTSTSLDVKDAGFSSPE
                     PEGFIKTAPTHARAFMSRINTTEHVSMRFGNESYTSPDLNPDTSGNYAIGTLSGLADG
                     KYWVNVSGTDTFGQERSLNWNFTLDTTKPVIDKFVITDSDGDGYIEAGEKLYLTWDVT
                     DTYFDNVTLVDVATGEKLWSSIASSGNNVEVQPIPIGNRNLAFRAYDKAGNSASSGFH
                     LYYDYTIWVNSTKMGEVVGINTTYTAMKDLTRTGISSVTLYGCTVTLPALNQLQKNVT
                     NVGQVTPDTYVTVDKNANVTISGSDTYRQAWVYESNKDLDFLVKVPYARNATLVLAEA
                     NETYIAELIKGGKGGMHSVNYTELIKKTAYIFIEGGWAQITVGDNGIITPGPKSGNDL
                     TISTEGISATLRDADNQVNLDVAGYRLSTQKDRTGTSLNAIKLQSGDYALAAIAMDGD
                     RLGVLAAMPVVVMESETQGWLSADSVVQGGNITARFATPCERLGVVVLRDVAYDGNAL
                     IDAATLGKETLHLNLTYNGIPATQKLIGNVYVSPSSGKYAVSNTNQTTFSTSGLEPGT
                     YRVYMIGQSAKGTVQAYGQHMLNITSPVPPTPTPGPTPYHRGGGGSSGSTTASYTGTG
                     TLLTGSSGTVLKSIIVNANDNVGSVLVPIGTKALDADGKPLGEITLKPLAGDAVPAVP
                     SGSVFKFAGYAYEASPDGATFSPGITLSLSIPEDVWNSLDLTNQQCVMKWYNKETGLW
                     EDVPTTVIPGTRTVEIRVTHFSIYALFTEPVTTPTPTETATTTPTTTTTTPSGDQPAE
                     GLPMMMILAIFAVIAIIVVAGYFLMMRK"
     gene            2892..5084
                     /locus_tag="PLI76_08050"
     CDS             2892..5084
                     /locus_tag="PLI76_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62023.1"
                     /translation="MSIEENFDIPLIAAMALKEKQIQQNYRPIIAVHKWFARRPGTLF
                     RGLILSEFGGRPVADTFFQANSIPDRTIADPFMGGGTPLLEANRVGCNVVGFDINPMA
                     AWIVREEIERLDLQEYRSEAERLITSLEQEIGELYRTDCNLYGDSDVPVKYFLWVKTL
                     DCEECGASIDLFPGYVIAKDARHPRNVVVCSCCGELNEISDLQAPGACRCCGEKLLST
                     GPARRNRCVCPDCGYQNTYPRPESGPPRHRLFAIEYHNPYRAGGHEGRFFKKPDEKDL
                     DMVQEAARRLRSIRPRYIPEQKILAGDETDRLHHWGYNHYRELFSGRQLLGLELSCRR
                     IAGVPDDRIRRALATNLSDLLRYQNLLCRYDTTALKSLDIFSVHGFPVGLIQCESNLP
                     GITNGQGMNVGSGGWRNIVNKYITAKQYCEAPFETYQKGKRKVRIPIVGEWIGGSPDA
                     CGRREVHLYCANSAGVKLKPASLDAVFTDPPYFGNVQYGELMDFCYVWLRGLAGTGAE
                     GFDRESTRSPEELTGNESQGRDLEHFADGLSSVYRNMVYALKPGAPLAFTFHHNNVET
                     YYAVGVAILDAGLRCFASLPCPAEMGGSIHIHGTKSSIVDTVFVCRRYGHTRRGQDFE
                     TLVQLAGIVRDELEQLKRAGMKPTEGDIRCIVYGHLTRMTVWRLSEGWNSTLPVGERL
                     NRFAETFSGFGDLQPVLDLLARHLRSAAPCNLPVQTRLYEEGRDAVSL"
     gene            5053..6105
                     /locus_tag="PLI76_08055"
     CDS             5053..6105
                     /locus_tag="PLI76_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62024.1"
                     /translation="MRRAVMPFPFEVPFEEVQSDLESYIDATFDSLGSAFLTMPRGEG
                     FTEYPTFEEGYEVLKRATRNFRDMSPESVTAAVYDAPIAFIVLRTILGFTPPEWAYVT
                     TQRTGVEVTQGAARGIDKKIRTNPFEPLCAPNSGITDIRIQAMVQVACELIREGPREN
                     IAGILHRLDKADTRQGLNSLQSLAGLGVPYPMLLYERFLGRPFAAHRDSVSGLIGDVL
                     EAAIEEVLSRGGISFRKTKRAERVIGFDQAPDFIIPDEFNPQIVIEAKIAEDDGTARD
                     KVTRVQHLGELSMQGRRAGEAPKYEVIACIAGRGFGIRREDMKKLILSTRGKVFTLST
                     LDRLIECTKLKEYAIR"
     gene            6559..7134
                     /locus_tag="PLI76_08060"
     CDS             6559..7134
                     /locus_tag="PLI76_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62025.1"
                     /translation="MKRVFGILALMVIASIMIAGCVQPTGNETATPGTTTPGVTETPM
                     ATTTTETPMATTTTETPMATTTTGTPATTATPMETGTTTATPTTAAANVTETPPGPPA
                     AGGETVSITDSGFVPDTLTISPGTTVTWTNDAATNETITATGQAAYFDSGMLQPGDSY
                     NYTFTAPDNYSYMSQTSGFTGTVVVTGNTTS"
     gene            complement(7147..>7905)
                     /locus_tag="PLI76_08065"
     CDS             complement(7147..>7905)
                     /locus_tag="PLI76_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinic acid a,c-diamide synthase"
                     /protein_id="HOI62026.1"
                     /translation="MAAETGVLGGFGAVVEETCDLSGIMDLARSAPPLPAPPGTRDRP
                     EARVRVGIANDAAFCFYYADNLDRLARAGADLIFFSPMTDRLPDVDALYLGGGYPELH
                     AAALAASRCREDVRQAADDGMPVYAECGGLIYLTERLTTGDGDYPMAGILPAAAVMTD
                     RIQALGYVEARVVGGAPVLTPGSAFRGHEFHYSRLDCAPDARFALELLRGKGIDGGRD
                     GLTAQNAVGQYTHAYITEESAGTLVRAAAAYRRS"
BASE COUNT         1726 a         2437 c         2322 g         1420 t
ORIGIN      
        1 cttcagcaaa caggtaacct ggccggtctt tgagcccatc acgctgaccg tcgaggcgac
       61 tgacgccgca gggaattaca attcgacatc cacatccctt gatgtaaagg acgccggatt
      121 ctcatcgccc gagccggaag gcttcatcaa aaccgccccg acccatgccc gggcgttcat
      181 gtcccggata aatacgaccg aacatgtctc gatgcgtttt ggtaatgagt cgtacacatc
      241 accggatctg aaccctgaca cttccggcaa ctacgcgatc ggcaccctgt ctggtcttgc
      301 cgacgggaag tactgggtga acgtcagcgg caccgacacc ttcggtcaag aacggtccct
      361 gaactggaac ttcacgctcg acactaccaa gccggtgatc gataagttcg tcatcaccga
      421 cagcgacggt gacggctaca tcgaggccgg cgagaagctc tacctgacgt gggacgtcac
      481 cgacacgtac tttgataatg tcacccttgt ggatgtcgca acgggcgaga aactctggag
      541 cagcattgcc tcctctggaa ataacgtcga agtacaaccg atcccaatcg gaaaccgcaa
      601 tctcgcgttc cgtgcctacg ataaggccgg caactccgcc agcagcggat tccacctcta
      661 ctatgactac acgatctggg tgaactcgac gaagatgggt gaggtcgtcg gcatcaacac
      721 gacctacacc gcgatgaaag atctcacccg gacagggata agctccgtca ccctctatgg
      781 gtgcactgta acccttcctg ccctgaacca actgcaaaag aacgttacaa atgtcggtca
      841 ggtcaccccc gatacctacg tgaccgtgga caaaaacgcc aacgtaacca ttagcggaag
      901 cgacacctac cggcaagcct gggtctacga atcgaacaaa gacctcgact tcctggtgaa
      961 ggtgccgtac gccaggaacg ccaccctcgt gctcgcggag gcgaacgaga cctacatcgc
     1021 cgaactgatc aaaggcggaa agggcggcat gcactcggtc aactacaccg agctcatcaa
     1081 gaagactgcc tacatcttca tcgagggcgg ctgggctcag atcacggtgg gggacaacgg
     1141 tataatcact ccggggccca aatccggtaa tgacttgacc atctctaccg aaggcatctc
     1201 tgcgaccctc cgcgatgctg acaaccaggt gaacctggac gttgccggct accggctaag
     1261 cacccagaag gaccggactg gcacgtccct gaacgcgatc aagcttcagt ccggcgacta
     1321 cgcccttgct gcaatcgcca tggacggcga ccggctcggt gtcctcgccg cgatgcctgt
     1381 cgtagtcatg gaaagtgaaa cccagggctg gctctctgcc gacagtgtgg tgcagggagg
     1441 taacatcacc gcgaggtttg cgacaccctg tgagcgcctt ggggtggttg tcctccgcga
     1501 tgtcgcctat gacggcaacg cgctcatcga cgcagcgacg ctcggtaaag aaaccctcca
     1561 cctgaacctt acttacaacg gtatccccgc gacccagaag ctcatcggca acgtctacgt
     1621 cagcccgagc agcgggaagt acgccgtatc gaacacgaac cagaccacgt tctcgacgtc
     1681 gggacttgag cctggcacct accgggtcta catgatcggg cagagcgcga aggggacggt
     1741 gcaggcctat ggtcagcaca tgctcaacat cacctcgcct gtgccgccca cgcccacgcc
     1801 cggccccaca ccctaccacc gcggcggtgg cggcagcagc ggcagcacca ccgcctccta
     1861 caccgggacc ggcaccctgc tgaccggcag ctcgggcacc gtcctcaagt ccatcatcgt
     1921 gaacgccaat gacaacgtcg ggagcgtgct cgtcccgatc ggcacgaagg cgctcgatgc
     1981 cgacggcaaa ccgctcggcg agatcaccct caagcctctc gccggcgacg cggtcccggc
     2041 ggtcccgtcc ggatcggtct tcaagtttgc cggctacgcc tacgaggcca gccccgacgg
     2101 tgcaaccttc agcccgggca tcacgctctc gctcagtatc cccgaggacg tctggaactc
     2161 cctcgacctc accaaccagc agtgcgtgat gaagtggtac aacaaggaga ccggcctctg
     2221 ggaggacgtc ccgacgaccg tcatcccggg cacgaggacc gtcgagatcc gggtgacgca
     2281 cttcagcatc tacgcgctct tcaccgagcc ggtgacgacc cccaccccga ccgagacggc
     2341 gaccacgacc ccgacgacga caacaacgac accgtccggg gatcagcccg ctgaagggct
     2401 gcccatgatg atgatcctcg cgatctttgc ggtgatcgcc atcatcgtag tggccggata
     2461 cttcctcatg atgcggaagt aatccagcaa ctcttttttt ggtcccgtgg acccgtaaac
     2521 ggcacgtgaa tgcccccccg gcgaagtccc tgcgacgccc cgggaagggg tgtttcgcgg
     2581 tcggtcgccc gggagggagg gccggatggt ttttcggccg gattgatcgc ggaacatgac
     2641 ggctacaggc gagatcgccg gagaagttgt acgagaacgc gcctccgatc tgaaccgcgt
     2701 tttcacgttc cggtgtggga aagtctaaat cgtagagtat cgagatcata acctggcgga
     2761 tgccataatc cgttttgacg ggtatatctg cgcctcctgc ctcgagctgc atgaagagag
     2821 ccggatcatg gcaacacgcc cggaaacatg ccctgccgga gcagaacggc ccgggacaaa
     2881 aagaggggac aatgagcata gaagaaaatt tcgacatacc gctgattgcc gcaatggcgc
     2941 tcaaagaaaa acagatccag cagaattacc ggccgattat tgcggttcat aagtggtttg
     3001 ccagaaggcc cggcacgttg ttccgcggtt tgatcttatc ggaattcggc gggcgcccgg
     3061 ttgccgatac gttcttccag gctaacagca tccccgatcg tacaatcgcc gatccgttca
     3121 tgggaggagg caccccgctc cttgaagcaa accgtgtggg gtgcaatgtc gtaggtttcg
     3181 acatcaatcc catggccgcc tggattgtac gcgaggagat agagcgcctc gacctccagg
     3241 agtatcgaag tgaggccgaa aggttaataa catcgttaga acaggagata ggggaactct
     3301 acaggaccga ctgcaatctc tacggggata gcgacgtccc ggtaaagtac tttctctggg
     3361 tcaagacact tgactgcgaa gagtgcggcg cgtccatcga tctgttcccc gggtacgtga
     3421 tcgcaaagga tgcgcgtcat ccccgtaacg tcgtggtgtg ctcgtgctgc ggagaactca
     3481 atgagatcag cgatctgcag gcgccgggtg catgcaggtg ctgtggagag aaactgctct
     3541 cgaccggccc tgcaaggcgt aaccggtgcg tctgcccgga ctgcggatac cagaatacct
     3601 atccccggcc ggaatccggc cccccacgtc accggttgtt tgcgattgag tatcataacc
     3661 cgtacagggc gggtgggcac gagggtcggt tcttcaagaa acccgatgaa aaagacctgg
     3721 atatggtgca ggaagccgcg cggaggctac gctcgatccg cccgcgctac atcccggagc
     3781 agaaaatttt agcgggagat gagaccgaca ggctccatca ttggggttac aaccattacc
     3841 gggagttatt cagcgggcgg caactgctcg gtctggagtt gagctgccgt cgcatcgccg
     3901 gggttccgga tgaccgcata aggcgggctc ttgctacaaa cctgtccgat cttctccggt
     3961 accagaatct gctgtgccgt tacgacacca cggccctgaa gtccctggat atcttctcgg
     4021 ttcatggatt tcccgtcgga ttgatccagt gcgaatcgaa tctcccggga attacgaacg
     4081 ggcagggcat gaacgtgggt tcgggcggat ggcgaaatat cgtcaacaaa tacataacgg
     4141 ccaagcaata ctgcgaagct cccttcgaaa cctaccagaa aggaaaacga aaggtccgca
     4201 tacccattgt gggcgaatgg attggaggta gtccggacgc ctgcggccgg cgggaggtgc
     4261 atctgtattg cgcaaattcc gccggcgtaa aactgaagcc cgcaagcctg gatgcagtgt
     4321 tcaccgatcc cccgtacttc gggaacgtcc agtatggcga gttgatggac ttctgctacg
     4381 tctggttgcg gggccttgcc ggaaccggtg cagaaggatt tgatagggaa tcgacccgct
     4441 ccccggaaga attaaccggg aatgagagcc agggcagaga cctggagcat tttgcggacg
     4501 gtctctcgtc tgtctaccgc aacatggtat atgccctgaa gccgggagca ccgcttgctt
     4561 ttacgtttca ccacaacaac gtcgaaacat attatgccgt cggcgttgcg attcttgacg
     4621 ccgggctcag gtgctttgcg agtctgccct gtcctgccga gatgggggga tctatccata
     4681 tacacggcac aaaatcgtcg atcgtcgata ccgtttttgt ctgccggagg tatgggcata
     4741 cccggagagg ccaggacttc gaaaccctgg tacagctggc aggtatcgta cgggacgaat
     4801 tagagcaatt aaaacgagcg ggcatgaaac ccaccgaagg ggatatccgg tgcatcgtat
     4861 acggacatct cacccgcatg accgtatgga gattatctga aggatggaac tcaacgctac
     4921 ctgttggtga gcggttgaac aggttcgcag aaacattctc gggtttcggg gatctgcaac
     4981 cggtcctgga tctgcttgcc cggcacctga gatctgcggc cccctgcaat ctaccggttc
     5041 aaacccgtct ctatgaggag ggccgtgatg ccgtttccct ttgaggtgcc gtttgaagag
     5101 gtgcaatcgg atctggagag ttacatcgat gcaacatttg attctctggg gtctgcgttt
     5161 ttaacgatgc ccagagggga agggttcact gaatatccca cgtttgaaga gggttacgaa
     5221 gtactgaagc gcgccacacg caatttccgg gatatgtcgc cggaatcggt gactgcagcg
     5281 gtatatgacg ccccgatcgc cttcatcgtg ctccggacga tccttggttt caccccaccc
     5341 gaatgggcat atgtcaccac acagcggaca ggcgtagagg taactcaggg cgctgcccgc
     5401 ggaatagaca aaaagatccg aaccaatccg tttgagccgc tgtgcgcccc gaacagcggt
     5461 ataacggata tacgaataca ggcgatggtg caggttgcct gtgagttaat ccgcgaaggt
     5521 ccccgtgaga acattgcggg tatactgcac cggctggata aggcagacac aagacagggg
     5581 ctgaacagcc ttcaatcact ggccggcctt ggtgtcccct acccgatgct cctctatgaa
     5641 cgatttctgg gaaggccttt tgccgcacat cgcgactccg tctccgggct gatcggggac
     5701 gtgctggagg ccgccatcga agaggtgttg tcgcgcggag ggataagttt ccgaaagacg
     5761 aaacgtgcag agcgcgtaat cggatttgat caggccccgg acttcattat tccggacgag
     5821 tttaatccgc agattgtcat tgaggcgaag attgccgagg atgacggtac agcgcgcgac
     5881 aaagtgaccc gggtgcaaca cctgggcgaa ctcagtatgc agggccgccg ggccggcgag
     5941 gccccgaagt atgaagtgat tgcctgtatt gccggccggg gtttcggcat ccgccgggag
     6001 gacatgaaga aactcattct ctcgacccgc ggaaaggtgt tcaccctgag taccctggac
     6061 cgcctgatcg agtgcacaaa attgaaggag tatgcgatac ggtaggatgg ggcgggaccg
     6121 tcacgaagcc tccctgttct ttctcagaag tcccccccgg acaaactgtt cgacatccgg
     6181 gccggcagcc cggagagtga gccggctgac gtgttagaac ccggccctcc gccggatccg
     6241 gtgcgcccgg ctcgacaccg cggcaacggg gacgggaaac ggatcgagat ggcgccatct
     6301 gcccgcccgg tgcagggggt gccgcaccgg gcgggatatg aacctccggc gacaggaccg
     6361 ggaaccccct atccgggctc atggcccgtt acccggagat ttgatgccgt ccggggtgct
     6421 tatcggccgt aaggcccacg ttattggtcg gatagcagaa cagccggagg caacgccttc
     6481 ccgacaaaca tttaccctgc attccgattt tcgcaccggg ggtaaagttt atctgcgcta
     6541 aggagcatca tccgctccat gaagagagtg tttggtatcc ttgccctcat ggtgatcgcg
     6601 agcatcatga tcgcagggtg tgtgcagccg acgggcaacg agacagcgac ccccggcaca
     6661 acaacaccgg gtgtcaccga gaccccgatg gcaacaacga caaccgaaac cccgatggca
     6721 acaacgacaa ccgagacccc gatggcaaca acgacaaccg ggaccccggc gacgacggcg
     6781 acgcccatgg agaccgggac caccacagca accccaacga ccgccgccgc gaacgtgacg
     6841 gaaaccccgc cgggaccgcc ggcagccggc ggcgagaccg tctccatcac cgatagcggt
     6901 tttgtgccgg atacgctcac catctccccg ggcaccacgg tgacctggac caacgacgcc
     6961 gccacgaacg agaccatcac ggcgaccggg caggcggcat acttcgactc agggatgctc
     7021 cagccgggcg actcgtataa ctacaccttc accgcaccgg acaactactc ctacatgtca
     7081 cagacatccg ggtttaccgg gaccgtcgtc gtcaccggca acacaacctc ctgacccttt
     7141 ttccctctat gaccgccggt atgccgcagc ggcccgcacc agcgtcccgg cagactcttc
     7201 cgtgatgtag gcgtgggtgt actgcccgac cgcgttctgc gccgtaagtc cgtcccggcc
     7261 gccgtcgatc ccctttcccc ggaggagctc cagggcgaac ctcgcgtcag gggcgcagtc
     7321 gagacgtgag tagtggaact cgtggccccg gaaggccgaa cccggcgtaa ggaccggggc
     7381 cccgccgacc acccgggcct ccacgtagcc gagagcctgg atccggtcgg tcatcactgc
     7441 cgccgcgggg aggatccccg ccatcgggta gtcgccgtcg ccggtcgtga gccgttccgt
     7501 gaggtagatg agcccgccgc attccgcgta gaccggcata ccgtcatcgg ccgcctgccg
     7561 cacgtcctcc cggcatcggg aagccgcgag cgccgccgcg tggagttccg ggtaaccgcc
     7621 gccgaggtag agggcatcca cgtcggggag ccgatccgtc atcggcgaga agaagatcag
     7681 gtccgccccg gcccgcgcga gccggtcgag gttgtcggca tagtagaagc agaacgccgc
     7741 atcgttcgcg atgccgaccc gcacccgggc ctccggccgg tcccgggtcc cgggcggcgc
     7801 cgggagaggc ggggccgatc gcgcaaggtc catgatcccg gagagatcgc aggtctcctc
     7861 caccaccgcc ccgaaccccc caagcacccc ggtctcggcc gccat
//
LOCUS       DAOSEE010000101         7870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_3398_length_7870_cov_255.301216, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000101 DAOSEE010000000
VERSION     DAOSEE010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7870
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3398_length_7870_cov_255.301216"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1836
                     /locus_tag="PLI76_08070"
     CDS             <1..1836
                     /locus_tag="PLI76_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOI62027.1"
                     /translation="TTSRYAVVALSALAIGGASVYLLVRYASGGAVYFAASSEATDIV
                     MVAIEMALALFIIVMGVKFRNYLAIALAVVQAALVVYLETTFAGSLHAANNLFVDQFS
                     IIMALIIGIIGSLIAVYGVRYMETYHHHHPEVRDRQRMFFFVIFAFLAAMFGLVFSNN
                     LMWVFFFWEVTTIASFVLIGYSETEEATTNAFRALVMNLLGGVAFVAALIFLAATGPT
                     SGGVDLALVLASGDAAVILVPAVLIGFAGITKAALMPFSSWLLGAMVAPTPVSALLHS
                     STMVKAGVYILVRFAPVFAGTFAGLSVGLVGAVTFVVASAIAISRSDAKSVLAYSTIA
                     NLGLIAACAGVGTYMLVWAAILLIIFHAVAKSLLFLGTGAIEHRIGSRNIEDMEGLIV
                     RMPKMAVMMFIGIAGMFLAPFGMLVSKWAAIEAFVQAPFGLVFIAILAYGSAVTVFFW
                     AKWMGKLVAVTRGTERIEKGFLEEPWVPLYITTAIVILVVFLFPVISTALIEPYVFVI
                     YGMTAHLAQANVAIMLLMISLLLILPISFSLFRKSAKRLPVYMGGRPATPELQFAGSL
                     GMTREAQTQNYYLTGYFGEARLYWPGAVVCIVLILASWVFAGVAL"
     gene            1833..2699
                     /locus_tag="PLI76_08075"
     CDS             1833..2699
                     /locus_tag="PLI76_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOI62028.1"
                     /translation="MNWLWLIGAVLFLVLAPVAGGLIAGIDRKITARMQGRVGPPILQ
                     PFYDVGKLFEKERVVVTTAQNFYVLAYLVFIALSGAIFFAGGDLLLTVFAFTLAHVFL
                     VLGAYAVHSPYSHIGAERELIQVMAYEPMIILVVVGFYMVTGSFFVDAIAAATVPAIA
                     YLPGVFIGFAAILTIKLRKSPFDISTSHHAHQEIVKGITTEFSGSTLAQIEIAHWYEN
                     IFLLGLVYLFFAWNPVIAIIAVAVTYVAEIYIDNVTARVRWQAALKSGWLAAALGIAN
                     LAILSYMMIGGA"
     gene            2705..3172
                     /locus_tag="PLI76_08080"
     CDS             2705..3172
                     /locus_tag="PLI76_08080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOI62029.1"
                     /translation="MAFLKRSPWILHYDASSCNGCDIEVLACLTPLYDIERFGIINTG
                     NPKHADILLITGGINEQNRPVVKNLYEQMPEPKVVVAIGACAATGGIFRECYNITGGV
                     DKVIPVDVYVPGCAARPEQIIDGVVTALGILEEKRMKMTGEATRPAAGVGEER"
     gene            3169..3555
                     /locus_tag="PLI76_08085"
     CDS             3169..3555
                     /locus_tag="PLI76_08085"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOI62030.1"
                     /translation="MTMVHEEQTTMAVAREDLLNEVERLKTDGYRLVQIGCTDIGGAY
                     EINYSFDKGYQFKNLRLTVGPDEEVTSISGIYWGAFVYENEVHDLFGIPVTGINIDFK
                     GTFIRTATKYPFSVTRRGGDDPCQNA"
     gene            3540..4619
                     /locus_tag="PLI76_08090"
     CDS             3540..4619
                     /locus_tag="PLI76_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HOI62031.1"
                     /translation="MSKRIVVPFGPQHPVLPEPIHLDLVLEDELVVDVVPSIGYIHRG
                     LEQLVLKREFTEYVYVAERICGICSFMHALCYSQGIEHIMNIEVPDRAKYLRTIWSEC
                     SRLHSHLLWLGLFADGMGFENLFMRSWQLRESVLDVLEDTTGGRVIQGTCKVGGVRRD
                     IGQEKLDEMHRTLDTFEENCRDLGDVFLNDGSVKYRLQGLSVIPRDDAYALGAAGPTA
                     RASGVAMDHRETGYAAYGDLGFKPVVETAGDCYARCAVRVRELYSSIDIIRRAIEGMP
                     EGPIDVKVKGTPDGEYFSRVEQPRGEVVHYLRGNGTKHLARARIRTPTLANIPILVKM
                     LPGIQLADVPVVVLSIDPCISCTER"
     gene            4627..5013
                     /locus_tag="PLI76_08095"
     CDS             4627..5013
                     /locus_tag="PLI76_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOI62032.1"
                     /translation="MGLFKMTKTVLRNLAGGPATRCYPAVPARTSPLTRGHVAFDPAT
                     CRSCGICSRRCPCEAIRLDKEAKVWEIDRMRCIACGDCVEGCPFESLTMEQEYFPPVT
                     EHVVEHYTITYVKPEKPAKKPAEESA"
     gene            complement(5079..6008)
                     /locus_tag="PLI76_08100"
     CDS             complement(5079..6008)
                     /locus_tag="PLI76_08100"
                     /EC_number="5.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0046033 - AMP metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphate isomerase"
                     /protein_id="HOI62033.1"
                     /translation="MILNGTAASIKSMEIRGAGRIARAAVEALADYARNLEVSDADAF
                     KREMQKAAEILTETRPTAVSLPNAVRYVMRALDSFESVEAAREAVLARAAEFIDHSEH
                     AVERIAEIGARHISDGDVLLTHCNSEAALGCILEAHRQEKEIEVYATEVRPRGQGLIT
                     IRALNDAGIRTNYIIDSAVRYFINDVDLVIVGADAIAVNGAVVNKIGTAQIAHAADEA
                     RTNVIVAAETYKFAPRTILGEKIAIEERDTGEVLPGTIAENLPFVRVRNPAFDVTPAE
                     YVDLIVTEMGAFPPELAYLVIRDYLGWKIEDLR"
     gene            6058..6900
                     /locus_tag="PLI76_08105"
     CDS             6058..6900
                     /locus_tag="PLI76_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOI62034.1"
                     /translation="MSVAVVFDSAGTLLRTYRVARDILREEMLIEVETTTITFGSDAR
                     VLVVLHLHSRDVIDAPPDQLLSTFFIERSVGFGVACSRRVIPAEEVANLLYADRCARI
                     SDMQACIREVWNCCKEESVVAMNSGVILNMDIPGIEFTVTTGGRPFDGAKETISELHR
                     MGVPAYIASGDRVTKLERMGDYLGIPRERVYGVATPSMKARIVEDLKEQYDKVVMVGD
                     AINDLHAFRKADLAVLTEQQSDRKPEVLYDCVDRVIRNVSEVPGIVAELLADTTAPGE
                     NATI"
     gene            6948..>7870
                     /locus_tag="PLI76_08110"
     CDS             6948..>7870
                     /locus_tag="PLI76_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOI62035.1"
                     /translation="MTPLITVKDLCMEFNGSVVLKDIHFEVAEGETVGIIGRSGAGKT
                     VLMHLMRGVDQPPSRGAIIYHIAACGNCDYIGVPSEAGKPCPACGSELTPLDIDLWAE
                     ENAPMKRRIMRRTAIMFQRTFALYGDDRVIENVLRALDDVGYPSEKAVGRAADLIDQV
                     RLSHRMMHIARDLSGGEKQRVVLARQLAKNPFMLFADEPTGTLDPETATLVHRMLIES
                     AQKNDMAMVVTSHFSSVIEDVADRAILLENGEIKEIGDPGEVIRRFMEDYSDVERYQP
                     GEVGEKLLVARDVIKRYLSVDRGVVRAVNGVS"
BASE COUNT         1461 a         2520 c         2409 g         1480 t
ORIGIN      
        1 acgacatcgc gatacgcggt ggtcgccctc tcggctctcg ccatcggcgg agcatcggta
       61 tacctgctcg tacggtatgc atccgggggc gcggtctact ttgccgcatc ctcggaggcg
      121 accgatatcg tcatggttgc catcgagatg gcgcttgcgc tcttcatcat cgtcatgggc
      181 gtgaagtttc gaaactacct cgcaatcgca ctggccgtcg tccaggcggc gctggtggta
      241 tacctcgaga cgacctttgc ggggagcctc catgcggcaa acaacctctt cgtcgaccag
      301 ttctccatca tcatggccct gatcatcggg atcataggga gcctcatagc cgtatacggc
      361 gtcaggtaca tggagaccta ccaccaccac cacccggagg tgcgtgaccg gcagaggatg
      421 ttcttcttcg tcatattcgc cttcctcgcc gcgatgttcg gcctggtctt ctccaacaac
      481 ctcatgtggg tcttcttctt ctgggaggtc accaccatcg cctcgttcgt cctgataggc
      541 tactcggaga ccgaggaggc gacgacgaac gccttccgcg ccctggtgat gaacctcctc
      601 ggcggggttg cgtttgtcgc ggcgctcatc ttccttgccg cgaccggccc gacgagcggg
      661 ggcgtcgatc tcgccctggt gcttgcctcc ggcgacgccg ccgtcatact ggtaccggcc
      721 gtcctgatcg ggtttgccgg catcacgaag gccgccctga tgcccttctc ctcctggctc
      781 ctcggggcga tggtggcccc gacaccggtc tcggccctgc tccactcgag caccatggtc
      841 aaggccggcg tctacatact ggtccggttc gcaccggtct ttgccgggac gttcgccggg
      901 ctctccgtcg gcctcgtcgg cgcggtgacc ttcgtcgtcg cctccgctat cgcgatatcg
      961 cgaagcgacg caaaatcggt cctcgcctac tcgacgatag caaacctcgg cctgatagcc
     1021 gcgtgtgcgg gcgtcgggac atacatgctc gtctgggcgg caatcctcct gatcatattc
     1081 cacgccgtcg caaagtcgct cctcttcctc gggaccgggg cgatcgagca ccggatcggg
     1141 agccgcaaca tcgaggacat ggaaggcctg atcgtccgga tgccgaagat ggcggtgatg
     1201 atgttcatcg ggatcgccgg catgttcctt gcgccgttcg gtatgctggt ctccaaatgg
     1261 gcggcgatcg aggcgttcgt ccaggcgccc ttcggcctcg tcttcatcgc catcctggcc
     1321 tacgggagcg ccgtcaccgt cttcttctgg gccaagtgga tgggcaaact cgtcgcggtc
     1381 acccggggca cggagcggat cgagaaaggg ttcctggagg agccctgggt tcccctctac
     1441 ataaccaccg ccatcgtcat actggtcgtc ttcctcttcc cggtcatatc cacggcgctg
     1501 atcgaaccct acgtcttcgt catatacggc atgaccgcac acctggcgca ggcaaacgtc
     1561 gccatcatgc tcctgatgat atccctgctc ctgatcctcc cgatatcgtt ctccctcttc
     1621 cggaagagcg caaagcgcct cccggtctac atgggcggga ggcccgcgac gccggaactg
     1681 cagttcgcag gctccctcgg catgacacgg gaggcccaga cgcagaacta ctacctcacc
     1741 gggtacttcg gcgaggcgcg gctctattgg ccgggcgcgg tggtctgcat cgtgctgatc
     1801 ctggcatcgt gggtctttgc gggggtggcc ttatgaactg gctctggctt atcggggcgg
     1861 tcctcttcct cgtcctcgcg cccgtcgcgg gcggcctcat agcggggatc gatcgtaaga
     1921 tcaccgcgcg gatgcagggg agggtcggac cgccgatcct gcagcccttc tacgatgtgg
     1981 ggaaactctt cgagaaggag agggtcgtcg tcaccacggc gcagaacttc tacgtcctcg
     2041 cctacctggt cttcatcgcc ctctccgggg cgatcttctt cgcgggagga gacctcctgc
     2101 tgacggtctt tgccttcacg ctcgcccacg tcttcctggt cctcggggcc tacgcggtcc
     2161 actcgcccta cagccacatc ggggccgagc gggaactgat ccaggtgatg gcctacgaac
     2221 cgatgatcat cctcgtggtc gtcgggttct acatggtcac ggggagcttc ttcgtcgacg
     2281 cgatcgcggc cgcgaccgtg ccggccatcg cctacctccc gggcgtcttc atcgggtttg
     2341 ccgccatcct cacgatcaaa ctcaggaagt ctcccttcga catatcgacg tcgcaccacg
     2401 cgcaccagga gatcgtcaaa ggcatcacga cggagttctc gggctcgacg ctcgcccaga
     2461 tcgagatagc ccactggtac gagaacatct tcctcctcgg cctcgtctac ctcttctttg
     2521 cctggaaccc cgtcatcgca atcatcgcgg tcgcggtcac gtacgttgcc gagatctaca
     2581 tcgacaacgt caccgcacgg gtccgctggc aggcagcgct gaagagcggg tggctcgcgg
     2641 cggcactcgg gatcgcgaac ctcgcgatcc tctcctatat gatgatcggg ggtgcatgag
     2701 tagtatggca ttcctgaagc gatcaccctg gatccttcac tacgatgcat ccagctgcaa
     2761 cgggtgcgac atcgaggtgc ttgcatgcct gacgcccctc tacgacatag agcggttcgg
     2821 catcatcaac accggcaacc cgaagcatgc ggacatcctg ctgatcaccg gcgggatcaa
     2881 cgaacagaac cggccggtgg tcaagaacct ctacgagcag atgccggagc ccaaagtcgt
     2941 cgtcgctatc ggcgcctgcg ccgcgaccgg cggcatattc cgggagtgct acaacatcac
     3001 ggggggcgtc gacaaagtta tccccgtcga cgtctatgtc ccgggatgcg cggcccggcc
     3061 ggagcagatc atcgacggcg tcgttacggc gctcgggatc ctcgaggaga aacggatgaa
     3121 gatgacaggg gaagcaacgc gacctgccgc aggggtgggt gaggagagat gacgatggtg
     3181 cacgaagagc agacgacgat ggccgtcgcg cgggaagacc tcctgaacga ggtcgaaaga
     3241 ctcaagaccg acggctaccg cctcgtccag ataggatgca cggacatcgg cggggcttac
     3301 gagatcaact actcgttcga caaaggctac cagttcaaga acctccgcct gacggtcggg
     3361 ccggatgaag aggtaacgag catatccggt atctactggg gagcgttcgt ctacgaaaac
     3421 gaggtgcacg acctcttcgg gatcccggtg accgggatca acatcgactt taaggggacg
     3481 ttcatcagga cggcaacgaa gtatccgttc agcgtcacgc ggcgaggagg tgacgacccg
     3541 tgtcaaaacg catagtcgtg ccgttcgggc cgcagcaccc ggtgctgccg gaaccgatcc
     3601 acctcgacct cgtcttagag gacgagctgg tggtggacgt ggttccctcc atcggctaca
     3661 tccaccgggg gcttgaacag ctcgtgctga agcgcgagtt caccgagtac gtctacgtgg
     3721 cggagcggat ctgcggcata tgcagtttca tgcacgctct ctgctactcc cagggcatcg
     3781 agcatatcat gaacatcgag gttcccgacc gggcgaagta tctccggacg atctggtcgg
     3841 agtgttcgcg tctccactcc cacctcctct ggctcggcct ctttgcggac ggcatgggtt
     3901 ttgagaacct cttcatgcgc tcctggcagc tccgcgagag cgtgctcgac gtcctcgagg
     3961 acaccaccgg cggccgggtc atccagggga cctgtaaggt cggcggggtc aggcgcgaca
     4021 tcgggcagga gaaactcgac gagatgcacc ggacgctcga caccttcgag gagaactgcc
     4081 gggacctcgg cgacgtcttc ttaaacgacg gatcggtgaa gtaccgcctc caggggctct
     4141 cggtcatacc ccgggacgac gcttacgccc tcggggcggc agggccgacc gcgagagcaa
     4201 gcggagtcgc catggaccac cgcgagaccg gatacgcggc ctacggggac cttggtttca
     4261 aaccggtcgt cgagacggcc ggtgactgct acgcccggtg tgccgtcagg gtcagggagc
     4321 tctactcgtc gatcgacatc atccggaggg cgatcgaggg gatgcccgaa ggacccatcg
     4381 acgtgaaggt gaagggaacg ccggacggcg agtacttctc ccgcgtggag cagccccggg
     4441 gcgaggtcgt ccactacctg cgcggcaacg gcaccaaaca cctcgcccgc gcccgcatca
     4501 ggacgccgac gcttgcaaac atacccatac tcgtgaagat gctccccggc atccagcttg
     4561 ccgatgtccc ggtcgtcgtc ctctcgatcg atccctgcat cagctgcacg gagaggtgac
     4621 ggcggcatgg gactctttaa gatgacaaag acggttctcc ggaaccttgc gggaggaccg
     4681 gccacccggt gctacccggc ggtcccggca cggacgagcc ccctcacccg ggggcacgtc
     4741 gcgttcgacc cggccacgtg ccgctcctgc ggcatctgct cgcggcggtg cccgtgcgag
     4801 gcgatccgcc tcgacaagga ggcgaaggtc tgggagatcg accgtatgcg ctgcatcgcc
     4861 tgcggcgact gcgtcgaggg ctgcccgttt gagagcctca cgatggagca ggagtacttc
     4921 ccgccggtga cggagcacgt ggtggagcac tacaccatca cctacgtgaa acccgagaaa
     4981 ccggcaaaga agccggccga agagagcgcg taagagagcc ggggcccgga cccccggcaa
     5041 agaccttttt attcggcaat cgggaggaac tatcgccttc accgcaggtc ttcgatcttc
     5101 cacccgaggt aatcccgtat gaccagatac gcaagctccg gcgggaacgc ccccatctcg
     5161 gtgacgatca ggtcgacgta ctccgcgggc gtcacgtcga aggccgggtt tcggacccgg
     5221 acgaaaggaa ggttctctgc tatagttccg ggcagcacct cgccggtgtc gcgctcctcg
     5281 atcgctatct tctcgccgag tatggtccgc ggcgcgaact tgtaggtctc cgccgcgacg
     5341 atgacgttcg tccttgcctc gtctgcggcg tgcgcgatct gggcggtccc gatcttgttc
     5401 acgaccgctc cgttcaccgc tatggcatcg gcgccgacga tgaccaggtc gacgtcgttg
     5461 atgaagtagc ggaccgccga gtcgatgatg tagttcgtcc gtatgccggc atcgttcagc
     5521 gccctgatgg tgatgagccc ctggccccgc ggccgcacct ccgtcgcgta gacctcgatc
     5581 tctttttcct gccggtgcgc ttcaaggata cacccgagag ccgcctcgga gttgcagtgg
     5641 gtcaggagga cgtcgccgtc ggatatatga cgagcaccga tctcggctat ccgctcgacc
     5701 gcgtgctccg agtgatcgat gaactccgcc gcccgcgcga ggaccgcttc ccgtgcggcc
     5761 tccacggact caaacgagtc gagggcccgc atcacgtagc gcaccgcgtt cgggagcgag
     5821 acggcggtcg ggcgggtctc cgtgaggatc tctgccgcct tctgcatctc ccgcttgaag
     5881 gcatcggcgt cggaaacttc cagatttcga gcgtaatcgg ccagcgcctc caccgccgcc
     5941 cgggcgatcc tgccggctcc ccgaatctcc atactcttta tgcttgccgc cgtcccattc
     6001 agtatcataa tcattcattc aagcgagcgc cacatagatg tattgacgtg agggtagatg
     6061 tcggtagccg ttgtttttga tagtgcaggc acacttctcc ggacttaccg tgtagcgcgc
     6121 gatatcctcc gcgaagagat gctcatcgag gtcgagacca cgaccatcac cttcgggtcc
     6181 gatgcacggg tgctcgtcgt cctccacctc cactcccgcg acgtcatcga tgcgcctccc
     6241 gaccagctcc tctcgacgtt cttcatcgag cggtcggtcg gtttcggcgt ggcctgctcc
     6301 cggcgggtca tccccgccga agaggtcgca aacctcctct acgccgaccg atgcgcccgc
     6361 atcagcgata tgcaggcctg catcagggag gtctggaact gctgcaaaga agagtctgtc
     6421 gtcgccatga acagcggcgt gatcctgaac atggatatac ccgggatcga gttcacggtg
     6481 acgaccggcg gcagaccgtt tgacggcgcg aaagagacca tcagcgaact gcaccgaatg
     6541 ggcgtccccg cctacatcgc gtcgggggac cgtgtcacga aactcgagcg gatgggcgac
     6601 tacctcggta tcccccggga acgggtctac ggtgtcgcca ccccctcgat gaaggcccgg
     6661 atcgtggaag accttaaaga acagtatgac aaagtcgtca tggtagggga tgccatcaac
     6721 gacctccacg ccttccggaa ggcggatctc gcggtgctca ccgagcagca gtccgatcgg
     6781 aagcccgagg ttttatatga ctgtgtcgat cgggtgatcc ggaacgtcag cgaggtgccc
     6841 ggtatcgtgg cggaacttct cgcggacacc acggcgcccg gggaaaatgc aacaatataa
     6901 accctaatat gataccacga gaagataact ttaacgagga actcaagatg accccactca
     6961 tcacggtgaa agacctctgc atggagttca acgggagcgt tgtactcaaa gacatacact
     7021 tcgaggttgc agaaggtgaa acagtcggca tcataggaag aagcggggcc ggcaaaaccg
     7081 ttcttatgca tctcatgcgg ggtgtagatc aacccccgtc acggggtgcg atcatctacc
     7141 atatcgccgc ctgcgggaac tgcgactaca tcggcgtccc gagcgaggcg gggaaaccat
     7201 gtcctgcctg cggaagcgaa ctcacgcccc tggatatcga tctctgggcc gaagagaatg
     7261 cgcccatgaa acggcggatc atgcgaagga ccgccatcat gttccagagg acgtttgccc
     7321 tctacggcga cgaccgggtg atcgagaacg tcctgcgggc gctcgacgac gtcggttacc
     7381 cctcggagaa ggcggtcggc agggcggccg atctcatcga tcaggtcagg ctctcccacc
     7441 ggatgatgca tatcgcccgc gacctctccg gcggcgagaa gcagcgggta gtgcttgccc
     7501 ggcagcttgc aaagaacccg ttcatgctct ttgcggacga accgaccggg acgctcgacc
     7561 cggagaccgc cacgctcgtc caccggatgc tcatagagag cgcgcagaag aacgatatgg
     7621 cgatggtggt cacgtcgcac ttctcaagcg tcatcgagga tgtcgcggac cgggcaatcc
     7681 tgcttgagaa cggcgagatc aaggagatcg gggacccggg ggaggtcatc cgccggttca
     7741 tggaggacta cagcgacgtc gagcgctacc agcccgggga ggtcggcgag aagctcctgg
     7801 tcgcccgcga cgtgatcaag cgctacctct cggtcgaccg gggcgtggtc agggcggtca
     7861 acggcgtctc
//
LOCUS       DAOSEE010000102         7858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_3408_length_7858_cov_269.662181, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000102 DAOSEE010000000
VERSION     DAOSEE010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7858
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3408_length_7858_cov_269.662181"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PLI76_08115"
     CDS             <1..404
                     /locus_tag="PLI76_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1616 domain-containing protein"
                     /protein_id="HOI62036.1"
                     /translation="GEKFTEFYILGPTGKAADYPTEFMSRTPQTVIIGIGNHESRDIT
                     YTVETFAVESGFDSAKNRPTVVSATLLDRFSVTVPDNRTVEQPYTFRIEDPDVDRLEF
                     LLFAEAPPEEIPLDSLIGAGYRNLHLQLRVH"
     gene            594..1088
                     /locus_tag="PLI76_08120"
     CDS             594..1088
                     /locus_tag="PLI76_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62037.1"
                     /translation="MKRGILVASLLILSLVAAPAGAFSADDLRITLDTDGSADVTFNY
                     TLSWLERIAVFFRIAEPEKELKNALETATGSPVTVTSAMSDSATFSIRNFAKVRSTDE
                     GSVYSTPALDFTGAQEVLDSYWFAPLVKADFSPDLTLVRFPDGHEETFANQYAIPALS
                     HTIP"
     gene            complement(1300..2538)
                     /locus_tag="PLI76_08125"
     CDS             complement(1300..2538)
                     /locus_tag="PLI76_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HOI62038.1"
                     /translation="MAAKGDTYYAWAADAACQERGECGGAVTALLTHALKSGMVDAVF
                     AVKKGSDIYDAVPTLITDPAEVAETAGSLHCGTLLLSKLIKKYLEGAENMRIAMPVKG
                     CDAMGLYELAKRNQVNLDNILMIGLNCGGSVSPVLARKMIREKFGVDPDDVVKEEIDK
                     GQFIIVTKDGQHKGISMDELEEEGFGRRSNCRRCKMKVPRQADLACGNWGVIGDKAGK
                     ATFIEVCSEKGANLLDAAVAAGAVKTDAANPKGIEIRGKVENAMLKLGDKWRKKDFES
                     LGEGKDRLKKIMNDTSRCIKCYACIENCPICYCVECSTRKPYLVTPGQVPPPFMFHLI
                     RYAHISDSCINCGQCEENCAMDIPNALFMHALQVDLEEMFGHVPGVDMELPVLALVEE
                     RTERKRLSDTGSDQIFDIFK"
     gene            complement(2541..4607)
                     /gene="fdhF"
                     /locus_tag="PLI76_08130"
     CDS             complement(2541..4607)
                     /gene="fdhF"
                     /locus_tag="PLI76_08130"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HOI62039.1"
                     /translation="MELKYVQTTCPYCGTGCSFNLVVKDGKVVGTQPYKRSPVNEGKV
                     CPKGTYAHEFVNSPDRLTKPLIKKDGKFVEATWDEAYDLIAQKFKSYKPDEIACLSSA
                     RVSNEENYLLMKLARGVFKTRHIDHCARLCHSSTVAGLAASFGSGAMTNSIGDIAESK
                     CVFVLGSNTFEQHPLIGRKIVQAKLNGGKVIVFDPRYTPTAKQADLYASFYSGTDVAI
                     LNCLMGEIIRNGWEDKEWVSTRAKGYEELKAVVLKDDYLPENVEKISGISAQDLRTAA
                     EWIGTAESAALLYSMGITQHTVGVDNVKSTANIQMLTGNIGKRGGGVNALRGQNNVQG
                     ACDMGALPVVFTGYQKVIDPAAHKKFADAWGFPDGICEPKNGYEVTVMMDVLTDNPGE
                     LKCMYIMGENPMLSDPDLNHVRHALESVEFLVVQDIFLTETAELADVILPATCYAEKE
                     GTQTSTERRVQMWRKAQDPPGEAKVDWQILCEVAAAMGYAEQFPYHSAEEIFNEVAAL
                     TPSYHGMTYERLNKPEALHWPCPTLEHPGTPILHIGKCSHPDGMGVMHAIEWKPPAEV
                     PDAEYPYILTTGRCIWHWHTGSMTRRSETLDTEVPTGWIEINTEDAKELGIQDKEMVR
                     AMTRRGSVNAPAKVTDEIKKGVMFMPFHFAECAANVLTHNALDPIAKIPEFKACAVKV
                     EKITEA"
     gene            complement(4816..5967)
                     /locus_tag="PLI76_08135"
     CDS             complement(4816..5967)
                     /locus_tag="PLI76_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HOI62040.1"
                     /translation="MAAIGDMFYTWAADADVQKRGECGGAITALQQYALKSGMVDAVL
                     AVRKGADIYDAVPTVITDPAEVAETAGSLHCGTLLLSKLVKNYLDSNKTARLGVTVKG
                     CDTMGLIELAKRNQVDLDRVILLGVNCGGSVSPVLARKMIREKFEVDPDIVHKEEIDK
                     GQFIIEYEGGHKGIKIDDLEDEGYGRRSNCRRCLYKVPRQADLACGNWGVIGEKAGKA
                     TFVEVCSEKGANLLDAAIKAGAVMTEPANPKGIEIRGKVEGAMLNLGNKWRKKDFGAL
                     ASDLWGTISRETGRCIKCYSCIENCPVCFPVAKDFKGGSRMIASGEVPPNPMFHLRRF
                     THISDSCINCGQCEELCPMEIPLALFSHAIRTEGDSAFESKLGKAPYSN"
     gene            complement(5970..>7858)
                     /gene="fdhF"
                     /locus_tag="PLI76_08140"
     CDS             complement(5970..>7858)
                     /gene="fdhF"
                     /locus_tag="PLI76_08140"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HOI62041.1"
                     /translation="LTKPLIKKDGKFVEATWDEAYDLIAQKFKSYKPDEFAALASARV
                     SNEENYLMMKFARGVMKSRHVDHCARLCHASTVAGLAASFGSGAMTNSIGDIAESKCV
                     FVLGSNTLEQHPLIGRKIVQAKKNGAKVIVADPRYTPTARLADLYMPFVSGSDVALLN
                     GMMQEIIRNGWEDKEFIEKRTKDYEKLKEVVMKETYSLENVSKISGIPVEQLKQAAEM
                     FGTTKPGAILYSMGITQHTVGVDNVRSVANIQMLTGNLGKPGSGVNALRGQNNVQGAC
                     DMGALPVVFTGYQKVIDPAAYKKFADAWGFPGGICEPKNGYEVTVMMDVLTDNPGELK
                     CMYIMGENPMLSDPDLNHVRHALESVEFMVVQDIFLTETAELADVVLPAACYAERGGT
                     QTSTERRVQMWRKAQDPPGEAKEDWQIISELAARMGYAAQFPYKDIEEIFDEVAVLTP
                     SYHGMNYERLNRPEALHWPCPDASHPGTPILHREKFSHPDGIGIFAPIEWKPPAEVPD
                     AEYPFVLTTGRVLWHWHTGSMTRRSATLDAEVPTGWIEINPEDAKALGIKDKEKVRAV
                     TRRGSVDVPAKVTPDIMKGVMFMPFHFAECAANILTNNALDPIAKIPEFKACAVKVEK
                     ITEA"
BASE COUNT         1386 a         2331 c         2455 g         1686 t
ORIGIN      
        1 agggggagaa gttcaccgag ttctacatcc tcgggccgac ggggaaggca gccgattacc
       61 cgaccgagtt catgtcccgg acgccgcaga cggtcatcat cgggatcggg aaccacgagt
      121 cacgggacat cacctacacg gtggagacgt ttgccgtgga gagcgggttt gatagtgcga
      181 aaaaccggcc gacggtcgtc tcggcgacgc tccttgaccg gttctccgtc acggtgccgg
      241 ataaccggac cgtcgaacag ccgtacacct tccggataga ggatccggat gtggaccggc
      301 ttgagttcct cctctttgcg gaggcgccgc ctgaggagat accgctggat agcctcatcg
      361 gggcgggcta ccgtaacctg cacctccagc tccgggtgca ctgacgggcg cgaagggtta
      421 tcgggggcac caggggcccc ggatggcagg aggccccttc gccggatttt tacagttttc
      481 tcttttttct gcgagcgatg ccggaaaatt tccgatatcc ggccacgata cgcaggggca
      541 accctccaaa tatttatacc gcatgacggc gtatggttca tcgaggtata aggatgaaac
      601 gcggaattct ggtcgcctcc ctcctgatcc tgtcgcttgt ggcagccccg gccggggcgt
      661 tcagcgcgga cgacctccgg ataactctgg atacggacgg gagcgcagat gtcaccttca
      721 actacacact ctcgtggctc gagaggattg ccgtcttctt ccgtatcgcc gagcccgaga
      781 aggagctcaa gaacgcgctc gagacggcaa ccgggtctcc ggtaacggtg acctcggcca
      841 tgagcgactc cgccacgttc tcgatccgga actttgcaaa ggtcaggagc accgatgagg
      901 gcagcgtcta ctcgacgccg gcactcgact tcaccggggc gcaggaggtg ctcgacagtt
      961 actggtttgc accgctggtc aaagccgact tctccccgga cctgacgctg gtcaggttcc
     1021 ctgacggcca cgaagagacc ttcgcgaacc agtacgcaat cccggctctc tcccacacaa
     1081 tcccctgaac cggcccctat cctttctttt ttcactcaca caagatagag aggtacggcc
     1141 tctcaccccg ggcgctggag tcgggtctaa cacccgggag gtatgacgat gcccggcgat
     1201 gaatcgtata gaatcacttg cgttgccgcg aagacgagca gatcacacgc gatgaaccgc
     1261 ccaccacgga tgtcaaaaaa atagagggga aaactcccct tacttgaaga tgtcgaagat
     1321 ctggtcgctg ccggtgtcgg agagacgctt cctctccgtc ctctcctcga cgagtgcgag
     1381 gaccgggagt tccatatcca cacccgggac gtggccgaac atctcctcaa gatcgacctg
     1441 cagggcgtgc atgaagagcg cgttcggtat atccatcgcg cagttctctt cgcactggcc
     1501 gcagttgatg catgagtcgg atatgtgggc gtaccggatc aggtggaaca tgaacggcgg
     1561 cggaacctga ccgggcgtga ccaggtaggg cttcctcgtg ctgcactcga cgcagtagca
     1621 gatcgggcag ttctcgatgc aggcgtagca cttgatgcac cgggacgtgt cgttcatgat
     1681 cttcttcagg cggtccttcc cctcaccgag gctctcgaag tccttcttcc gccacttatc
     1741 gccgagcttc agcatcgcgt tctcgacctt gccgcggatc tcgataccct tcgggttcgc
     1801 ggcatcggtc ttgacggcgc cggcagcgac agccgcatcg aggaggtttg cacccttctc
     1861 ggagcagact tcgatgaacg tggccttccc ggctttgtcg ccgatgacac cccagttccc
     1921 gcaggcgagg tcggcctggc gcggaacctt catcttgcac cggcggcagt tcgaacggcg
     1981 gccgaaccct tcttcctcga gttcgtccat cgatatgccc ttgtgctggc cgtccttcgt
     2041 cacgatgatg aactgaccct tgtcgatctc ttccttgacg acgtcgtcag gatcgacacc
     2101 gaacttctcg cggatcatct tgcgggccag gaccgggctg accgagccac cgcagttgag
     2161 gccgatcatc aatatattgt ccaggttgac ctggttgcgc ttcgcaagct catagagccc
     2221 catcgcatcg cagcccttta ccggcatggc gatccgcatg ttctcggcgc cctccaggta
     2281 tttcttgatc agcttcgaga gcagaagcgt tccacagtgg agcgaacctg ctgtttctgc
     2341 gacctcggcg gggtcggtga tgagggtcgg aaccgcatcg tagatatccg aacctttctt
     2401 gactgcaaag acagcgtcca ccatgccgga cttgagcgcg tgcgtcagca gagcggtgac
     2461 cgctccgccg cactcgcccc tctcctggca ggcggcgtcg gctgcccatg cgtagtatgt
     2521 atctcctttc gctgccatct tcaggcctcc gtgatcttct cgaccttcac ggcacaggcc
     2581 ttgaactcgg ggatctttgc gatcgggtcg agggcgttgt gggtcaggac gtttgccgcg
     2641 cattcggcga agtggaacgg catgaacatg acgcccttct tgatctcgtc ggtcaccttc
     2701 gcgggcgcgt tgacggatcc acggcgggtc atcgcgcgga ccatctcctt atcctggatg
     2761 ccgagttctt ttgcatcttc ggtgttgatc tcgatccagc ccgtcggaac ctcggtgtcg
     2821 agggtctcag agcggcgggt catggacccg gtgtgccagt gccagataca gcgcccggtg
     2881 gtaaggatgt aggggtactc cgcgtcgggg acttccgccg gcggtttcca ctcgatggcg
     2941 tgcatcacgc ccataccgtc ggggtgggaa cacttgccga tgtgcaggat gggcgtgccg
     3001 gggtgttcca gggtcgggca gggccagtgg agtgcttcgg gcttgttcag ccgctcgtag
     3061 gtcataccgt ggtaggatgg cgtgagggcg gcgacctcgt tgaagatctc ctcagcacta
     3121 tggtagggga actgttctgc gtagcccatg gcggctgcca cctcgcagag gatctgccag
     3181 tcgaccttcg cctcgcccgg cggatcctgg gccttccgcc acatctggac acgccgctcg
     3241 gtggatgtct gagtgccctc cttctccgcg tagcaggtgg cagggagaat gacatcggca
     3301 agttcggcgg tctcggtcag gaagatatcc tggacgacca ggaactccac gctctcgagg
     3361 gcatgccgga cgtggttgag gtccgggtcg gagagcatcg ggttctcgcc catgatgtac
     3421 atgcacttga gttcgccggg gttgtcggtc aggacgtcca tcatgacggt gacctcgtag
     3481 ccgttcttgg gctcgcagat gccgtcgggg aagccccagg cgtcggcgaa cttcttgtgg
     3541 gcggccgggt cgatgacctt ctggtagccg gtgaagacga ccgggagcgc acccatgtcg
     3601 caggcgccct gaacgttgtt ctggccacgg agagcgttca cgccgccacc acgcttgccg
     3661 atgtttccgg tcagcatctg gatgttcgca gtcgacttga cgttatcgac gccgacggtg
     3721 tgctgggtga tgcccatcga gtagaggagc gcggcggact cggcggtacc gatccactcg
     3781 gctgcggtcc tgaggtcctg agcggatatg ccggagatct tctcgacgtt ctcagggagg
     3841 tagtcatcct tgagcacgac cgctttgagt tcctcgtatc cctttgcacg ggtcgagacc
     3901 cattccttgt cctcccagcc gttccggatg atctcgccca tcaggcagtt gaggatggcc
     3961 acatcggtgc cggagtagaa ggaggcgtag aggtcagcct gcttggcggt cggggtgtaa
     4021 cgcgggtcaa agacgatgac ctttccaccg ttcagtttgg cctgcacgat cttgcggccg
     4081 atgagcgggt gctgctcgaa tgtgttggac ccgagaacga agacgcactt ggactcggcg
     4141 atgtcgccga tggagttcgt catcgcaccg gaaccgaacg atgcagcaag gccggcgacg
     4201 gtggacgagt ggcagagccg ggcgcagtgg tcgatatgac gcgtcttgaa gacaccacgt
     4261 gccagtttca tcagcaggta gttctcttca tttgagacac gagcggagga gaggcaggcg
     4321 atctcatcgg gcttgtagga cttgaacttc tgcgcgatca ggtcgtaggc ctcatcccag
     4381 gtcgcctcga caaacttgcc gtccttcttg atgagcggct tcgtgaggcg gtccgggctg
     4441 ttcacgaact cgtgagcgta ggtgcctttg gggcagacct ttccctcgtt gaccggagaa
     4501 cgtttgtaag gttgtgtgcc gacaaccttc ccgtccttca cgacgaggtt gaagctgcat
     4561 cccgtaccgc agtacgggca tgtcgtctgt acatacttaa gttccatggt aaaacctcgg
     4621 atatgtaaaa atacgaggtc aatcatattt aatagtagtg attaacaccc ccatttttga
     4681 tgaataagtg tgggttgcgc cacaccgatt ttcttgaaaa acatacaata ccgggtataa
     4741 ccacctgaat accgattcag gggatcatcg ctattctgag taatgatgcg ctgcccgtac
     4801 ggcgggctaa aaaaattagt tggagtaggg tgccttcccg agcttcgact cgaatgcgct
     4861 atcgccctcg gtccttatgg catgggagaa gagtgcaagc ggaatctcca tcgggcagag
     4921 ttcctcgcac tggccgcagt tgatgcacga gtcggatatg tgtgtaaacc gccggaggtg
     4981 gaacatcggg tttggcggaa cctcgccgga tgcgatcatc ctcgagcctc ccttgaagtc
     5041 ctttgcaacc gggaagcaga cggggcagtt ctcgatgcag gagtagcact tgatgcaccg
     5101 gcccgtctcg cggctgatgg tgccccagag gtccgatgcg agcgccccga agtccttctt
     5161 ccgccacttg ttgccgaggt tgagcatggc cccctcgacc ttgccgcgga tctcgatccc
     5221 cttcgggttt gcgggttcgg tcatgacggc accggccttg atggcggcat cgaggaggtt
     5281 cgcacccttc tcggagcaga cctcgacgaa cgtcgccttc ccggccttct cgccgatgac
     5341 gccccagttc ccgcaggcga ggtcagcctg gcgcgggacc ttgtagaggc accggcggca
     5401 gttcgaacgg cggccgtaac cctcgtcctc gaggtcgtcg atcttgatcc ctttgtgccc
     5461 accctcgtac tcgatgatga actggccctt gtcgatctcc tctttgtgga cgatgtcggg
     5521 atcgacctcg aacttctcgc ggatcatctt ccgggccagg accgggctga ccgagccgcc
     5581 gcagttcacg ccgagcagga tgacgcggtc caggtcgacc tggttgcgct tcgcaagctc
     5641 gatgaggccc atcgtgtcgc agcccttgac ggtgacaccg agccgtgcgg tcttgttgct
     5701 gtccaggtag ttcttgacga gcttcgagag caggagcgtt ccgcagtgga gcgagcctgc
     5761 cgtctctgcg acctcggcgg ggtcggtgat gacggtcggg accgcatcgt agatgtccgc
     5821 gcccttccgg acggcaagga ccgcgtccac catgccggac ttaagggcat actgctgcag
     5881 ggcggtgatc gcgcctccgc attcgcctct cttctgcacg tcggcgtctg ccgcccacgt
     5941 gtagaacata tcgccaattg ctgccatctt caggcctccg taatcttctc gaccttcaca
     6001 gcacaggcct tgaactccgg gatcttcgcg attggatcga gagcgttgtt cgtcagtatg
     6061 tttgccgcac actcggcgaa gtggaacggc atgaacatga cacccttcat gatgtcgggg
     6121 gtcaccttcg cggggacgtc gaccgaacca cggcgggtga cggcacggac cttctctttg
     6181 tccttgatgc cgagcgcttt tgcgtcctcg gggttgatct cgatccagcc cgtggggact
     6241 tcggcgtcaa gcgtcgcgga ccggcgggtc atgctgccgg tgtgccagtg ccagaggacg
     6301 cgcccggtcg tgaggacgaa cgggtactcg gcatcgggga cttccgccgg cggtttccac
     6361 tcgatcgggg cgaatatgcc gatgccgtcg gggtgggaga acttctcgcg gtgcaggatc
     6421 ggcgttcccg ggtggctggc gtcagggcag ggccagtgga gggcctcggg cctgttcagc
     6481 cgctcgtagt tcatgccgtg gtaagatggc gtgagaacgg cgacttcatc gaagatctcc
     6541 tcgatgtcct tgtaggggaa ctgggcggcg tagcccatcc gggcggcaag ctcgctgatg
     6601 atctgccagt cttcctttgc ttcgcccggc ggatcctggg ctttgcgcca catctggacg
     6661 cgccgctcgg tggacgtctg ggtgccgccg cgttcagcgt agcatgcggc gggtaaaaca
     6721 acatcggcaa gttcggcggt ctcggtcagg aagatatcct gaacgaccat gaactccacg
     6781 ctctcgaggg cgtgccggac gtggttgagg tccgggtcgg agagcatcgg gttctcgccc
     6841 atgatgtaca tgcacttgag ttcgccgggg ttgtcggtca ggacgtccat catgacggtg
     6901 acctcgtagc cgttcttcgg ctcgcagata ccgccgggga aaccccaggc gtcggcgaac
     6961 ttcttgtagg cggccgggtc gatgactttc tggtagccgg tgaagacgac gggcagagct
     7021 cccatgtcgc aggcgccctg gacgttgttc tggccgcgga gggcgttcac gccgctgccg
     7081 ggcttaccca ggttgccggt gagcatctgg atgtttgcaa ccgaccggac gttgtcgacg
     7141 ccgacggtgt gctgggtgat acccatcgag tagaggatcg cgccgggctt ggtggtgccg
     7201 aacatctcgg cagcctgctt gagctgctcg acggggatgc cggatatctt cgagacgttc
     7261 tcaaggctgt aggtctcctt catgacgact tccttgagct tctcgtagtc cttcgtccgc
     7321 ttctcgatga actctttgtc ttcccagccg tttctgatga tctcctgcat catgccgttt
     7381 aaaagagcga catcggagcc ggagacaaac ggcatgtaga ggtcggccag ccgggcggtc
     7441 ggtgtgtagc gcgggtcggc aacaatgacc ttcgcaccgt tcttcttcgc ctggacaatc
     7501 ttacgcccga tcagggggtg ctgttcgagt gtgttcgacc cgagaacgaa gacgcacttg
     7561 gactcggcga tgtcgccgat ggagttcgtc atcgcaccgg agccaaacga cgcggcaagg
     7621 ccggcgacgg tggatgcgtg gcagagccgg gcgcagtggt cgacgtgtcg cgacttcatg
     7681 acgccgcggg cgaacttcat catcaggtag ttctcttcgt tcgagaccct cgccgacgca
     7741 agcgctgcga actcatcggg cttgtaggac ttgaacttct gcgcgatcag gtcgtaggcc
     7801 tcgtcccagg tcgcctcgac aaacttgccg tccttcttga tgagcggctt ggtgagac
//
LOCUS       DAOSEE010000103         7842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_3415_length_7842_cov_283.320791, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000103 DAOSEE010000000
VERSION     DAOSEE010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7842
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3415_length_7842_cov_283.320791"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1505)
                     /gene="uvrC"
                     /locus_tag="PLI76_08145"
     CDS             complement(<1..1505)
                     /gene="uvrC"
                     /locus_tag="PLI76_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOI62042.1"
                     /translation="MIDPQKLPTEPGCYLFSDDEGTIIYVGKAKNLKRRVSSYFSRHD
                     LDPKTRRLVAAIAGIDFIVTDTEVEAFILENTLIKKHQPKYNIDLKDSKSFAYIHITD
                     EVYPRIHVARGPDGSGRYYGPFVSARERDDLLRLLKSIFGLRSCRRLPRRACLRAHIG
                     SCSAPCTGAIGEEDYRERVKRAEAVLKGDIAGLIRTLREEMAVLAGRQEYERAMELRD
                     QIAALEGLRERQHVDRQKTYNEDIVNYIVSEGTVFLMLFNVDRGTLAGKREYTFDETE
                     GFLEEFLARYYAENEPPEEVILPEAVDDAITGYLSHLRGGRVRAVVPQRGEKKNLLDL
                     VRKNVEIGFFGDRIKLEELKRRLHLPDIPVAIECFDISHLSGTAMVGSMVRFLRGQPD
                     RRNYRRFRIRTVEGIDDFAAIAEVVRRRYSRLQKEGGELPDLILVDGGKGQLAAAVAV
                     LQELGIKVPIAAIAEREEEVFVPGFSHPLPLARNEKASLFLQEIRDEAHRFA"
     gene            complement(1502..4306)
                     /gene="uvrA"
                     /locus_tag="PLI76_08150"
     CDS             complement(1502..4306)
                     /gene="uvrA"
                     /locus_tag="PLI76_08150"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOI62043.1"
                     /translation="MKKIVIKGAREHNLQNITVELPRDRLIVLTGVSGSGKSTLAFDT
                     IYAEGQRRYVESLSAYARQFLGLMRKPDVDSIDGLSPAISIEQKTTSKNPRSTVGTVT
                     EIYDYLRLLFARVGTPFCPEHHIPIEAQSPEKIADHIAATMAGTVTILAPVVRQKKGT
                     YQQVFKDLDREGYTRVRVNGEIRRTDEEIALERYQKHDIEVVIDRLAADDRSRLVEAV
                     ENALAKSEGLVIAVDEDGREEICSSLMACPVCGIAFEELQPRMFSFNSPFGACEECNG
                     LGIRMEFDPDLIIPDRSKCIADGAVALYRNFLDGYRSQYLGAVAKHFGFSVLTPIRDL
                     TDRQYNALMFGSPEHLRLSMQMRNGDAWSRSGAWEGLAPQAERLYHQTGSDYRRQELE
                     KFMRVLPCPKCGGKRLKEKVLAVKVNGRSIVDVADLSIAGALAFFLTLELTESEREIA
                     KQVLKEIIARLEFLEQVGVGYLTLSRSAGSLSGGEAQRIRLATQIGSNLTGVLYVLDE
                     PSIGLHQRDNKKLLETLQRLRDLGNTLVVVEHDEETIRSADWVVDMGPGAGLHGGKVV
                     AEGPPDAIAGDPASLTGRYLSGDLRIDIPAARRGSDRFIRLLGCRGNNLKNIDVNIPI
                     GVLTVVTGVSGSGKSTLIYETLYRALMRELHGSREPPGEHDSLTFDDEIDKVIVIDQS
                     PIGRTPRSNPATYTKVFDEIRKVFAGTKEAKVRGYKPGRFSFNVKGGRCEACQGDGLI
                     KIEMNFLPDVYVECEECKGARYNRETLEVKYRGKSIADVLEMSVEEAIALFENVPSIR
                     NRLDTLSRVGLGYIKLGQSSTTLSGGEAQRIKLTRELAKRATGKTIYLLDEPTTGLHF
                     HDVKNLIAVLDDLVARGNTMVVIEHNLDVIKSADYVIDLGPEGGDAGGEVVAAGTPEE
                     VAAVEGSHTGAFLKEILKTP"
     gene            complement(4509..4964)
                     /locus_tag="PLI76_08155"
     CDS             complement(4509..4964)
                     /locus_tag="PLI76_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOI62044.1"
                     /translation="MKVEDFQKIITDAIDSEVESYTFYKSVSERVKDANLRSLFLELA
                     EDEKGHREYLQGLLLRGAKEMHFAAVSDYKVTDAIEEPALSVDMKPLDGLILAIKKEL
                     HAAQMYTRLAGASADAGQKMIFEQLANMEKGHKARLEDIYTNMAYPEAW"
     gene            5300..6016
                     /locus_tag="PLI76_08160"
     CDS             5300..6016
                     /locus_tag="PLI76_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5591 domain-containing protein"
                     /protein_id="HOI62045.1"
                     /translation="MAGRRSAGAPRQITGVVEDRHGNLIDIFDPPFYRREFEDAYRFI
                     IDDYVMAEKEIALFLPCAVRKPYSQSPSHRLFRRIIDGALDPTEYQIVIFGTCGTVPA
                     ELECMYPYRNYHYMLGKTTDGRIRRDFHRIEVYRLKGFLEKTCDTYRQRLAYCIGPFR
                     KAMVEASEETGIAVDLLPSDPMIERLYDIDCPFPEGSLSMQGYIDEFREGLARLSRSV
                     EKSSPPVIERHDRGRRPLRA"
     gene            complement(6043..7335)
                     /gene="rbcL"
                     /locus_tag="PLI76_08165"
     CDS             complement(6043..7335)
                     /gene="rbcL"
                     /locus_tag="PLI76_08165"
                     /EC_number="4.1.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016984 - ribulose-bisphosphate
                     carboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III ribulose-bisphosphate carboxylase"
                     /protein_id="HOI62046.1"
                     /translation="MAIDWYEEFVDTDHTPGPDELVALYYFEPAKGISREEAVGRIAS
                     ESSTGTWTTLFTMPPRMRNLQAKAFDIEGNYVKIAYPLALWEEGNAPQLLSGIAGNVF
                     GMKALERLRLIDASLPAEYIRHFKGPHFGIEGIRDMMKVRDRPLTGAVPKPKVGFTAE
                     EHAAVGYETWMGGFDFVKDDENLTSLAFNRFDDRVRAMTKLRDKAEQETGDVKSALIN
                     ITADTETMEERAKMLADYGWSYAMIDVVVAGTAAVATLRDYCSDLGLAIHAHRAMHAA
                     FDRDEKHGITMQFLAKIMRLVGVSQIHTGTAVGKLVGTRAEATVLADMLRETHMNAVN
                     HMALDQDWGGIKSAFPVSSGGLHPGLVPDVLDIYGSELVLLVSGGIHGHPKGTRAGAE
                     ATMQAIEAWQEGETLDEKAKKAPELREALDKWGYYKPR"
BASE COUNT         1276 a         2450 c         2530 g         1586 t
ORIGIN      
        1 gcgaaccggt gcgcctcgtc gcggatctcc tggaggaaga gcgacgcctt ctcgtttcgc
       61 gcaagcggca gggggtggga gaaccccggc acgaagacct cttcttcgcg ctccgcgatg
      121 gccgcgatcg gaaccttgat cccgagttcc tggagcacag cgacggccgc cgcgagctgc
      181 cccttcccgc cgtcgacgag gatcaggtcg gggagttcgc cgccctcttt ctggaggcgc
      241 gagtagcggc ggcggaccac ctcggctatg gcggcaaagt cgtcgatgcc ctcgaccgtc
      301 cgtatcctga aacgccggta gttccgccgg tcgggttgtc cccggaggaa ccggaccatc
      361 gaccccacca tcgccgtccc ggagaggtgg gatatatcga agcactcgat cgcgacgggg
      421 atatccggga ggtgcaggcg gcgtttcagt tcctccagtt tgatccggtc gccgaagaac
      481 ccgatctcga cgttctttcg caccaggtcg agcaggttct tcttctcgcc ccgctgcggc
      541 acgaccgccc ggaccctgcc gccgcgaagg tgggagaggt agccggtgat ggcgtcgtcc
      601 accgcttcag ggaggataac ctcctcgggc ggctcgttct ccgcgtagta ccgggcaagg
      661 aactcctcga ggaacccctc cgtctcgtcg aacgtgtact cgcgcttccc ggcaagcgtt
      721 ccccggtcga cgttgaagag catgaggaat acggtcccct cgctcacgat gtagttgacg
      781 atatcttcgt tgtaggtctt ctgccggtcc acgtgctggc gctcgcgcaa tccctcgagg
      841 gctgcgatct ggtcgcggag ctccatcgcc cgctcgtact cctgccgccc tgcgaggacc
      901 gccatctctt cccggagcgt ccggatgagt ccggcgatat ctcccttcag gaccgcctcg
      961 gccctcttca cccgctcccg gtagtcttct tcgccgatgg ctcccgtgca gggggcgctg
     1021 cacgaaccga tgtgagcccg gaggcacgcc cgccggggaa ggcgcctgca ggaccggagg
     1081 ccgaagatcg actttaagag ccggaggagg tcgtcgcgct cccgtgcgga gacgaacggc
     1141 ccgtagtagc ggccgctccc gtcgggtccc cgggcgacgt ggatacgggg gtagacctcg
     1201 tcggtgatgt ggatgtaggc gaagctcttc gagtccttga gatcgatgtt gtacttcggc
     1261 tggtgcttct tgatcagggt gttctcgagg atgaacgcct cgacctcggt atcggtgacg
     1321 atgaagtcga tcccggcgat cgcggcgacg agccgtctgg tcttcgggtc gaggtcgtgc
     1381 cgggagaagt aacttgatac gcgccgcttg aggttctttg ccttcccgac gtagatgatg
     1441 gtgccctcgt cgtcggagaa gaggtagcac ccgggctcgg ttggaagttt ctggggatcg
     1501 atcatggtgt tttaaggatc tcttttaaga acgcgcctgt gtggctcccc tccaccgcag
     1561 cgacctcctc gggggttccg gccgcgacga cctcaccgcc ggcgtccccg ccctccggac
     1621 caaggtcgat gacgtagtcg gcggacttga tcacgtccag attatgctcg atcacgacca
     1681 tcgtgtttcc ccgggccacc aggtcgtcga ggacggcgat caggttcttc acgtcgtgga
     1741 agtgcagccc ggtcgtcggt tcgtcgagga ggtagattgt cttccctgtg gcccgctttg
     1801 cgagttcccg ggtcagcttg atccgctgcg cctcgccgcc cgagagggtg gtggaactct
     1861 ggccgagttt gatgtagccg agccccaccc gggagagcgt atcgagcctg ttcctgatcg
     1921 aggggacgtt ctcgaagagg gcgatcgcct cctcgacgct catctcgagg acgtcggcga
     1981 tggacttgcc ccggtacttc acctcgaggg tctcgcggtt gtagcgcgcc cccttgcact
     2041 cctcgcactc gacgtagacg tcggggagga agttcatctc gatcttgatg agcccgtcgc
     2101 cctggcaggc ctcgcaccgt ccgcctttga cgttgaacga gaaccggccg ggcttgtagc
     2161 cccggacctt cgcctccttg gtcccggcaa agaccttccg gatctcgtcg aagaccttgg
     2221 tgtaggtcgc cgggttcgag cgcggcgtcc gcccgatggg gctctggtcg atgacgatca
     2281 ccttgtcgat ctcgtcgtcg aacgtgaggc tgtcgtgctc ccccgggggt tcccgcgaac
     2341 cgtggagttc ccgcatcagc gcccggtaga gcgtctcgta tatgagcgtg gacttccccg
     2401 accccgagac cccggtgacg accgtcagga cgccgatggg gatgttgacg tcgatgtttt
     2461 ttaagttgtt cccccggcag cctagaaggc ggatgaaccg gtcgctccct ctccgggcgg
     2521 cgggtatgtc gatccggagg tcgcccgaga ggtagcgccc ggtgagggag gccggatctc
     2581 cggcgatggc gtcgggcggc ccctccgcga cgaccttgcc gccgtggagc ccggccccgg
     2641 gccccatatc cacgacccag tcggcgctcc ggatcgtctc ctcgtcgtgc tccacgacga
     2701 ccagggtatt cccgaggtcg cggagacgtt gcagggtctc gaggagcttt ttgttgtcgc
     2761 gctggtgcag cccgatcgag ggttcgtcga ggacgtagag caccccggtg aggttcgatc
     2821 cgatctgggt tgcaaggcgg atcctctgcg cttccccgcc cgagaggctg cctgcgctcc
     2881 gcgagagggt caggtagccg acgccgacct gttcgaggaa ctcgagtcgg gcgatgatct
     2941 cctttaagac ctgttttgct atctcgcgtt cgctctcggt gagttccagg gtcaaaaaga
     3001 acgcaagcgc tcccgcgatg gagaggtcgg cgacgtcgac gatggatctc ccgttcactt
     3061 tgacggcgag aaccttctcc ttgagcctct ttcccccgca cttcgggcag gggaggaccc
     3121 gcatgaactt ctcgagttcc tggcggcggt aatccgaccc ggtctggtgg tagagccgct
     3181 cggcctgcgg agcaagcccc tcccatgccc cggagcgcga ccacgcgtcc ccgttcctca
     3241 tctgcatgga gaggcggagg tgctcgggcg aaccgaacat cagggcgttg tactgacggt
     3301 cggtgagatc ccggatcggt gtcaggaccg agaacccgaa gtgtttcgcg acggcgccga
     3361 ggtactggct ccggtagccg tcgaggaagt tccggtagag cgcgacggcg ccgtcggcga
     3421 tgcatttgct cctgtccggt atgatcaggt cgggatcgaa ctccatcctt atcccgagtc
     3481 cgttgcactc ctcgcaggcc ccgaaggggc tgttgaagga gaacatacgg ggctggagtt
     3541 cctcaaacgc gataccgcag accgggcagg ccatgagcga cgaacagatc tcctcgcgcc
     3601 catcctcgtc gacggcgatg acgagccctt ccgactttgc aagggcgttc tcgacggcct
     3661 cgacgagcct tgagcggtcg tcggcggcaa ggcggtcgat gaccacctcg atgtcgtgct
     3721 tctggtagcg ttcgagggcg atctcctcgt cggtccggcg gatctcaccg ttgacccgga
     3781 cccgggtgta gccttcccgg tcgaggtcct tgaagacctg ctggtaggtc cctttcttct
     3841 gccgcacgac cggggcaagg atggtgaccg tcccggccat ggtggcggct atgtggtccg
     3901 cgatcttctc gggcgactgg gcctcgatgg ggatgtggtg ctccgggcag aacggtgttc
     3961 ccacccgggc aaagaggagc cggaggtagt cgtagatctc ggtgaccgtg ccgacggtgc
     4021 tccgggggtt tttggatgtc gtcttctgtt cgatggatat ggcgggcgag agcccgtcga
     4081 tgctgtcgac gtccggtttc ctcatcaggc cgaggaactg ccgcgcgtag gcggagagcg
     4141 actccacgta gcgccgctgg ccttcggcgt agatggtgtc gaaggccagc gtggattttc
     4201 ccgacccgga gacgccggtg aggacgatga gccgatcgcg cgggagttcg acggtgatgt
     4261 tctggaggtt gtgctcccgc gcccccttga tgacgatctt cttcattctt atgtgcagat
     4321 cagtttgtct tcttacttca tagccgtttg ccgacaccaa catttaaata aaatgatgga
     4381 aacgagcccg gtatacagga ggttttgagg gtaaagattg aggaagcggt ccgatcccgg
     4441 gccgtcgcgc ggccgggccg ggtccacctg cagaagagcg atagagaggg gttttgaggc
     4501 ggcggtggtt accacgcctc ggggtatgcc atgttcgtgt agatatcctc gagccgggcc
     4561 ttgtgcccct tctccatgtt tgcaagctgc tcaaagatca tcttctggcc ggcatcggca
     4621 gaggcgccgg cgagccgggt gtacatctgt gcagcgtgca gttccttctt gatggcgagg
     4681 atcaggccgt cgagcggttt catgtcgacc gagagcgcgg gctcctcgat agcatcggtg
     4741 accttgtaat cgctgaccgc tgcaaagtgc atctcctttg caccgcggag cagcaggccc
     4801 tgcaggtact cccggtggcc cttctcgtcc tcggcaagtt cgaggaagag gctccgcagg
     4861 ttcgcgtcct tcaccctctc ggatacggat ttgtagaagg tgtacgactc aacctcgctg
     4921 tcgatggcgt ccgtaatgat cttctggaaa tcttcgactt tcatagctgg tttctccttt
     4981 ctgcgccgtc ccggccctgt gggaggtcgg ctgcacggtt tgttcgtagt tatacgaaca
     5041 atactctcta tgaggggccg gatatttatt ctttttcttt tgcatccggc agaacctcca
     5101 taagggcccg gcatgggttt acggcacccg cccgttcctg aaaaggggga gtgcaacctg
     5161 tagagatccc ggcgcgtgcc gccggcaccc gggccccggc actaccggga tacccccctc
     5221 gcaaggccgg tgctttttcg ttcccggcgg ccaacaggta catgctgaag agcaaaccct
     5281 tttcatggtg gaggatagga tggcagggag acggagcgcc ggtgctcccc ggcagatcac
     5341 cggggtcgta gaggacaggc acgggaacct gatcgatata ttcgatcccc ccttctaccg
     5401 gagggagttt gaggacgctt accggttcat catcgacgac tacgtgatgg ccgagaagga
     5461 gatcgctctc ttcctgccct gcgccgtccg gaaaccctac agccagagcc cgagccaccg
     5521 gctcttccgc cggatcatcg acggcgccct cgatccgaca gagtaccaga tcgtcatatt
     5581 cggcacctgc gggaccgtcc ctgcggagct cgagtgcatg tacccctaca gaaactacca
     5641 ctatatgctc ggcaagacaa cagacgggcg aatccggcgg gatttccacc ggatcgaggt
     5701 ctaccggctg aaagggttcc tcgagaagac ctgcgatacc taccggcagc ggctcgccta
     5761 ctgtatcggg ccgttccgga aggcgatggt ggaggcatcg gaagagaccg ggatcgccgt
     5821 cgacctgctc ccctccgacc cgatgatcga gcggctctac gatatcgact gcccgttccc
     5881 cgaaggcagc ctctcgatgc agggctacat cgacgagttc cgggaggggc tggcgaggct
     5941 ctcgcggtcg gtagagaaga gcagcccgcc ggtcatcgag cggcacgatc ggggtcgcag
     6001 accgctacgg gcatgacggc gccgggtttg acgaaaaagt agttatctgg gtttatagta
     6061 gccccactta tcgagggctt ctctgagctc gggagccttc ttcgccttct cgtcgagggt
     6121 ctccccctcc tgccaggcct cgatcgcctg catggtcgct tccgcgcccg cccgggtgcc
     6181 cttcgggtgg ccgtggatgc cgccgctcac gaggaggacg agttcgctgc cgtagatatc
     6241 gaggacatcc ggcacgagcc cggggtggag tccgcccgac gagacgggga aagcgctctt
     6301 gataccgccc cagtcctggt cgagtgccat gtggttcacg gcgttcatgt gtgtctcccg
     6361 gagcatatcc gcaagcaccg tcgcctccgc ccgggtgccg accagtttcc cgacggcggt
     6421 cccggtgtgg atctgggaaa caccgacaag gcgcatgatc ttcgccagga actgcatcgt
     6481 gatgccgtgc ttctcgtccc ggtcgaaggc cgcgtgcatc gcccggtggg cgtggatggc
     6541 aagccctaga tcggagcagt agtcccggag ggtcgcaacg gcggcggtcc cggccaccac
     6601 gacgtcgatc atggcgtaac tccagccgta atccgcgagc atcttcgccc gctcctccat
     6661 cgtctccgtg tcggccgtta tgttgatgag cgcggacttc acgtcgccgg tctcctgctc
     6721 ggccttatcg cgcagtttcg tcatggcccg gacgcggtcg tcgaaccggt tgaacgcaag
     6781 ggacgtcagg ttctcgtcgt ccttcacgaa gtcgaatcct cccatccagg tctcgtagcc
     6841 gaccgcggcg tgctcctctg cggtgaaccc caccttcggt ttcggcaccg cccccgtgag
     6901 cggtctatcc cggaccttca tcatatcccg tatcccctcg atgccgaagt gcggcccctt
     6961 gaagtgccgg atgtattcgg ccgggagcga ggcgtcgatc aggcggaggc gttcgagcgc
     7021 cttcatgccg aagacgttcc cggcgatgcc gctcaagagc tggggtgcgt tcccttcctc
     7081 ccagagggcg agggggtagg cgatcttcac gtagttcccc tcgatatcga acgctttcgc
     7141 ctggaggttc cgcatccggg gcggcatggt aaagagcgtc gtccaggtcc ctgtcgagct
     7201 ctcggaggca atcctcccga cggcctcctc cctgctgatc ccttttgccg gttcaaagta
     7261 gtagagggct acgagttcgt ccggcccggg ggtatggtct gtgtcaacaa actcttcgta
     7321 ccaatcaatc gccatagaat cacctcttga aggaagggat gcccgggtag aaaaactttc
     7381 cattcgaggc cgggtggaga gaggcaaaat cacgttctcc ctgcaggcac cgggtttatc
     7441 cgccggaggc cgagatgaag gcagaaggtg tggtatgggg gagattgccg agaacgtgcg
     7501 ttctttgact atagtgcgga aaggccgccc gatatcgaga ttgccggcac cggcgcccgg
     7561 gcctccccgg cgaccccggc agaagccggc cggtcaaagc gctttgctgc catccttgtt
     7621 ctccacccgg gacccggctc ccgcgccgcg ttccttgccg gtatgggcgc ggtctccctg
     7681 ctcgtcgatg ctcccctggc ggagagaacg gtggcggcct ggggccgtgc agttacggac
     7741 ccggggggaa gccgtgcggg agcatatccg gacggcgctc gaccttcgac gggggcttga
     7801 cctccttata gcgcagccgg gcgccgaccc ggaacgaatc gg
//
LOCUS       DAOSEE010000104         7786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_3457_length_7786_cov_251.095201, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000104 DAOSEE010000000
VERSION     DAOSEE010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7786
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3457_length_7786_cov_251.095201"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..1250
                     /locus_tag="PLI76_08170"
     CDS             42..1250
                     /locus_tag="PLI76_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOI62047.1"
                     /translation="MKIIKDPVHGYVEADEPMLRLLDSGVVQRLRHVTQLGFANLVYP
                     GANHTRFEHSLGAMHLAGIMCRELGLDGEETRLVTTAALLHDVGHGPFSHVTEPVMEE
                     FVGRKHHQIGYLVREGAIAAILEAEGIDPAEVCAVVAGEHRFASIIHGSLDVDRMDYL
                     MRDAHYTGVPYGTVDAHRLIRCSVLAESGIALRESGINAAESLLIARTLMRPAVYFHH
                     VSRIATSMFVHALREEVLTVPGANARELVRMDDAACMERLKHSPSPIVRDLASRVYAR
                     DLYKRALYVGEDRVNAAALRQDPGPAREREIARDIAGVAGVPVAKVLVDIPPLPRALS
                     MEVRVRNGHAMVDIEEVSPLIGTLNDTRRQQWRLGVYTAPEHRQAVEQAATEVLRVKR
                     ATKQDKLVVT"
     gene            1265..1819
                     /locus_tag="PLI76_08175"
     CDS             1265..1819
                     /locus_tag="PLI76_08175"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HOI62048.1"
                     /translation="MKKEFVIGVTGASGVIYARRLLEVLCDRATVHIVVSDTARQIAG
                     IEGVDLTGFDAVYAENENLAADIASGSFRYDGMAIVPCSMKTLAAVSNGLADNLIARA
                     ADVCLKERRPLLLLLREMPLSRVHLRNMLAADEAGATVMVASPPFYQRPATIDDLVDM
                     VVARVLDHLGVEHALGSRWSGYDA"
     gene            1812..3053
                     /locus_tag="PLI76_08180"
     CDS             1812..3053
                     /locus_tag="PLI76_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HOI62049.1"
                     /translation="MRDFIAMMREQGRVEDIERSCSTIYEAPRMASRTDKILFFHDLD
                     GHRAVMNLLGDRKSLAAALGVGERDLVRNLAARTYNGRVVDAGRLAGGIPADLSRLPV
                     MKHFPGDAGRYFTAGVVFSRYGGVENASIHRMMVLDDHRVVARLVEGRHTHTLLKAAL
                     ARGERLPVAVTVGTHPLVTFAACTRVPEGKELPYAAELMGGELAVHECANGVRVPGAE
                     IVLEGSIGPETAAEGPFVDITGTYDPVRQQPVIEFSRMYLRENPIYHGILPAGDEHKL
                     LMGAPYEPKIYRAVGEVTTVRDVLLTKGGAGYLHGVVQIRKNTDGDAKNAIMAAFAAH
                     TSLKHVVVVDEDIDIHDPYDVEYAIATRVRGDTDIMIITGVRGSSLDPMRLADGTNVK
                     VGVDATMLMGREGEFRRAEWP"
     gene            3057..4217
                     /locus_tag="PLI76_08185"
     CDS             3057..4217
                     /locus_tag="PLI76_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HOI62050.1"
                     /translation="MYLNEDDERILAGEFGETRQKMLEILVALGNVYGAERLVPITSA
                     QVSGASYKTIGRWGLAWLQSLNARVAVPAVLNPIGMPREEWREFGIREEFAHNQGAVI
                     EAYRRLGIKLECTCTPYYLRITEYGEHLAWSESSAVAYANSVLGARTNREGGPSALAA
                     AIIGKTPYYGLHIVANRRPQVVVDVEGGTGLRSDHWGAIGHVAGRKVGNRIPIFAGIR
                     PNRDQLKALGAAMAATGAVALFHVDTITPEARVFSFDTGDLDRVTVGADEVETLFAEM
                     DVEAVAVGCPHCSAEELRELADLLRGKKTTKPFYIFAARGVAKSNPDLIAAIERSGAR
                     VIPDTCMVVSPRMDEFSSIMVDSGKALAYVPGMCGALARIGTRRECVEVATS"
     gene            complement(4329..5015)
                     /locus_tag="PLI76_08190"
     CDS             complement(4329..5015)
                     /locus_tag="PLI76_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62051.1"
                     /translation="MRRELLLCGILASILIVACAPAVTAAAVGPVMPRDPVGPTASRM
                     GNVIKPGTVLTYDASISRITTDQYGMSSQSYVTGFDWVIRVEEASGGRYAGTAEIVPR
                     SAGMTGGVYRWSYVEGQQNPDWYGPPLWVDPGAPAESAGTYGSSTWQLLGDPRGLRMP
                     NPSTGVEQSVNQMQTVGPGVLRYISYDTDYGTVLLYSEKTDQYFMSFWFRSLNEGGGG
                     LTGPFGGMVS"
     gene            5357..5626
                     /locus_tag="PLI76_08195"
     CDS             5357..5626
                     /locus_tag="PLI76_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOI62052.1"
                     /translation="MEEKDIDTLRRRRDIDGLIRALSDPAGIVRLTAAEALGSAGDER
                     ALEPLERLKFSDPDIGVRRAASLAHARVAARLAEKKAVEGWLLDR"
     gene            complement(5654..6745)
                     /locus_tag="PLI76_08200"
     CDS             complement(5654..6745)
                     /locus_tag="PLI76_08200"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HOI62053.1"
                     /translation="MEYPPPKPVSKAGPFRDIPVDILRGLAITFMIMANLTPALLLPP
                     APGWYRFLSSLAAPIFIGLAGMMVALSRTGKGRPFSYIATRGGLFILAGALLQVLIFD
                     YVPFLDVDVLYLIGLALPLTYLYLGLPERGRWAVIIAILCLTPLLQVGLGYSGAVITR
                     PDVTLTPFSISGDPFPAPVEIARAWLMEGWFPVFPWLAIAFFGAELGRYRWREGAAPR
                     FRFKDEGRWGAGLLLAGAVLWMLFPGAQYIRDSYVELFYPPMIGFFVFAAGLVVFALT
                     VLDHIPAQYGLLDPLRAAGGCSLAIYLAQYAVIVKVIEPFGLQLPLPAYLGVFLVLYT
                     GMILLAYFLRYVRTSWHGQPFLVRFLIGS"
     gene            6970..7248
                     /locus_tag="PLI76_08205"
     CDS             6970..7248
                     /locus_tag="PLI76_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOI62054.1"
                     /translation="MTAPEDIDAMRDARDVAGLIRALDDADEFVRAQAALSLGTIADP
                     EAREPLDRMRREDPSASAREAAATAYKWLVGRLEEVEAVRGPKPPPPW"
     gene            complement(7256..>7786)
                     /locus_tag="PLI76_08210"
     CDS             complement(7256..>7786)
                     /locus_tag="PLI76_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOI62055.1"
                     /translation="EPALVALIGASILLFWSRQPPEEIFEKIEWPALFFFGGLFIVVG
                     ALVETGTIAAVAGFVVESVHSEGEAMLIIAWFSAIASAVVDNIPLTATLIPLIHDMGA
                     SMNTYPLWWALSLGACLGGNGTAIGASANVVVLGIAARNGLTISFVEFLKVGMLVLFV
                     TVGIGTAILWLNFFVL"
BASE COUNT         1488 a         2473 c         2596 g         1229 t
ORIGIN      
        1 atacctttat cactcctctc ccaagaacct taacggaacg gatgaagatc atcaaggatc
       61 cggtgcacgg ctacgtcgag gcggacgaac cgatgctcag gctgctcgac tcgggagtcg
      121 tccagaggct tcgccacgtc acgcaactcg ggtttgcaaa cctcgtctac ccgggcgcaa
      181 accacacccg gtttgagcac tcgctcgggg cgatgcacct tgccggcatt atgtgccggg
      241 agctcgggct tgacggcgag gagacgcggc tcgtcacgac ggccgccctc ctccacgacg
      301 tcggtcacgg gcccttctcc cacgtcaccg aaccggtgat ggaagagttc gtcgggagga
      361 agcaccacca gatcggctac ctggtcaggg agggagcgat cgccgccatc ctcgaggcgg
      421 aggggatcga tcccgccgag gtctgcgccg tcgtcgccgg tgagcaccgc ttcgcgagca
      481 tcatccacgg gagcctcgac gtcgaccgga tggattacct gatgcgggac gcccattaca
      541 ccggcgtgcc ctacgggacg gtcgacgccc accgtctgat ccggtgttcg gtcctcgccg
      601 aatcaggcat cgcgctccgc gagagcggca tcaacgccgc cgaatcgctc ctcatcgccc
      661 ggaccctgat gcgccccgcg gtctacttcc accacgtgag ccggatagcg acgagcatgt
      721 tcgtccacgc cctccgcgag gaggtgctca ccgtcccggg cgcgaacgcc cgcgaactcg
      781 tgcggatgga cgacgccgcc tgcatggagc gtctgaagca ctcgccctct ccgatcgtcc
      841 gcgaccttgc cagccgggtc tacgcccggg acctctacaa gcgggcgctc tacgtcggcg
      901 aagaccgggt gaacgccgcg gccctccggc aggaccccgg gcctgcccgg gagcgggaga
      961 tcgctcgcga tatcgccggg gttgccggag tcccggtggc aaaggtcctc gtggatatcc
     1021 ccccgctccc gcgggcgctc tcgatggagg tccgggtcag gaacggccat gccatggtgg
     1081 atatcgagga ggtctcgccc ctcatcggca ccttaaacga cacccggcgg cagcagtggc
     1141 gcctcggggt ttacaccgcc ccggagcacc ggcaggcggt ggagcaggcg gcgaccgagg
     1201 tccttcgggt gaagcgggcg acaaaacagg ataaactcgt agtgacataa tagatggaga
     1261 aaagatgaag aaggagtttg tcatcggggt caccggggcg agcggggtca tatacgctcg
     1321 ccgcctgctc gaggttctct gcgatcgtgc aacggtgcac atcgtcgtat ccgacacagc
     1381 ccggcagatc gccgggatcg agggcgtgga ccttaccggg tttgacgccg tatacgccga
     1441 gaacgagaac ctcgcggcgg atatcgcgag cgggtcgttt cgctacgacg gcatggcaat
     1501 cgtcccctgc agcatgaaga cgcttgccgc ggtcagcaac ggccttgccg ataacctgat
     1561 cgcccgggca gcggatgtct gcctcaagga gcggcggccc ctcctcctcc tcctccggga
     1621 gatgccgctc tcccgggtcc acctgaggaa catgctcgcc gccgacgagg ccggcgcgac
     1681 cgtgatggtc gcaagccctc ccttctacca gcgcccggca acgatcgacg acctcgtcga
     1741 tatggtggtg gcccgggtgc tcgaccacct gggggtcgaa catgctctcg ggtcccgatg
     1801 gagcggatac gatgcgtgat ttcatagcga tgatgcggga gcagggccgg gtcgaggata
     1861 tcgagcggtc ctgctcgacc atctacgagg ctccccgcat ggcgagccgg accgacaaaa
     1921 tactcttctt ccacgacctt gacggccacc gggcggtgat gaacctgctc ggcgaccgaa
     1981 aatcgcttgc ggcagccctc ggcgtgggtg agcgcgacct cgtccggaac ctcgcggcca
     2041 ggacctacaa cggccgcgtc gtggatgccg gacggctcgc gggcggcatc cccgccgacc
     2101 tctcccgcct cccggtcatg aagcacttcc cgggcgatgc cgggcgctac ttcacggctg
     2161 gcgtggtctt ctcccgctac ggcggcgtgg agaacgcctc catccaccgg atgatggtcc
     2221 ttgacgacca ccgggtcgtc gcccgcctgg tggaggggag gcacacccac acgctcctca
     2281 aggcggcgct tgccaggggg gaacggctcc cggttgcggt caccgtcggg acccaccccc
     2341 tcgtgacgtt tgccgcctgc acccgtgtcc cggaaggaaa ggaactcccc tacgcggccg
     2401 agctgatggg cggagaactc gccgtccacg agtgtgcaaa cggcgtccgg gtccccggcg
     2461 ccgagatcgt gcttgaaggc tccatcgggc cggagacggc ggcggagggg ccgttcgtcg
     2521 acatcaccgg gacatacgac cccgtgcggc agcaaccggt gatcgagttc tcccggatgt
     2581 acctcaggga gaaccccatc taccacggca tcctccccgc cggcgacgag cacaaactcc
     2641 ttatgggagc cccgtatgag ccgaagatct accgcgcggt cggcgaggtg acgacggtcc
     2701 gcgacgtcct cctcacgaag ggcggggccg gctacctcca cggcgtggtc cagatccgga
     2761 agaacaccga tggtgatgca aagaacgcca tcatggcggc gtttgccgcc catacgtcct
     2821 taaaacacgt cgtcgtggtg gacgaggata tcgatatcca tgacccctac gacgtcgaat
     2881 atgcgattgc cacaagggtg cggggcgata ccgacatcat gatcatcacc ggcgtgcggg
     2941 gttcgtcgct tgacccgatg cgccttgcag acgggacgaa cgtgaaggtc ggggtcgacg
     3001 ccacgatgct tatgggtaga gagggcgaat tcaggagagc ggagtggccg tagttcatgt
     3061 acctgaatga agatgacgag aggatcctcg ccggcgagtt cggcgagacg cggcagaaga
     3121 tgctggagat cctggtcgcg ctcgggaacg tctatggagc cgagaggctc gtcccgatca
     3181 cgagcgccca ggtgagcggt gcgtcgtata agaccatcgg gaggtggggg cttgcctggc
     3241 tgcagagcct caacgcccgg gttgcggtcc cggccgtctt aaaccccatc ggcatgccgc
     3301 gggaggagtg gcgggagttc ggcatcagag aggagtttgc ccacaaccag ggggcggtca
     3361 tcgaggccta ccggaggctc gggatcaaac tcgagtgcac ctgcaccccc tactacctcc
     3421 gcataacgga gtacggggag catctggcct ggtcggagtc ttcggcggtc gcctacgcaa
     3481 actcggttct cggcgcccgg acgaaccggg agggcgggcc gagcgccctt gccgcggcga
     3541 tcatcgggaa gactccttac tacggccttc acatcgtcgc gaaccggcgg ccgcaggtcg
     3601 tcgtcgatgt cgagggcggc acgggtctgc gctccgacca ctggggcgcc atcggccacg
     3661 tggcgggcag aaaggtgggg aaccggatac cgatctttgc cggcatccgg ccgaaccgcg
     3721 accagttgaa ggcgctcggg gcggctatgg cggcgaccgg cgcggttgcg ctcttccacg
     3781 tcgacacgat cacgccggag gcccgggtct tttccttcga taccggcgac cttgaccggg
     3841 tgacggtggg cgccgatgag gtcgagaccc tcttcgcgga gatggatgtc gaggcggtcg
     3901 cggtcgggtg cccgcactgc tcggccgaag aactccgcga acttgccgat ctcctcaggg
     3961 gaaagaagac gacaaaaccc ttctacatct tcgccgcccg gggggtcgca aagagcaacc
     4021 ccgacctcat cgccgccatc gagcggagcg gcgcccgggt gatcccggat acctgcatgg
     4081 tcgtctcgcc ccggatggat gagttcagct cgatcatggt ggattcgggc aaagctctcg
     4141 cctatgtccc cggtatgtgc ggggcgctcg cccggatagg gacgcggaga gagtgcgtcg
     4201 aggtcgcgac gtcgtgagga gggccggcgc tccgtgtcgc cctccccccc cgcagaccgc
     4261 atgccgcctt tcgcggggtc gtcgggggtg tctgcgatcc gccgcaggcg ccgggatggc
     4321 ccggtgcgtt acgagaccat ccctccgaat ggcccggtca ggccgccccc gccctcattc
     4381 agagatcgga accagaacga catgaaatac tggtctgtct tctcgctgta caggagaacc
     4441 gtcccgtaat cggtgtcgta cgatatatac cgcagaaccc cggggcccac cgtctgcatc
     4501 tggttcacgg actgttcaac gccggtagaa gggttgggca tcctcaggcc ccggggatct
     4561 ccgagcagtt gccaggtgct gctcccgtat gtccccgcgg actccgccgg agctccgggg
     4621 tcaacccaga gcggagggcc ataccagtca gggttctgct gcccctccac atacgaccat
     4681 cggtacaccc ccccggtcat acccgcgcta cgcgggacga tctctgcggt ccccgcgtag
     4741 cggccaccgg atgcctcctc gacacgtata acccagtcga accccgtgac ataggattga
     4801 ctcgacatgc cgtactgatc ggttgtaatc cgggagatgg atgcgtcata cgtcagaaca
     4861 gtcccgggct tgatgacatt ccccatcctt gaagcagtgg ggcccaccgg atctctcggc
     4921 atcaccggtc ccaccgccgc cgccgtcacg gcgggtgcgc acgcgacgat aagaatgctc
     4981 gcgaggatcc cacacaacag caattccctt cgcatcagtc tcacatcctg caatcatcgt
     5041 tccacaccgg gtcatcctca ccgcgtccct ggtgccgcgc ggtggagcac catcccggcc
     5101 tccggaatgc gagcagtcga tgcgaacctg gtatatagac ctatgcgctc ccggccatgc
     5161 cggcacgaga tctccgcgaa gtcgttgccc ggccagtacc gatggccacc acgatatcgg
     5221 gagcatcgtg cgggtttcac cggccgttac cgggcgggca ggggtccggg ttgccgtcgt
     5281 gccgggatcg cctccggaac actgatgaga tggcgggcca taactgcacc gtaaagactg
     5341 gtcgaggaga tcctggatgg aagagaagga tatcgatacc ctgcggaggc gccgggatat
     5401 cgacgggttg atccgggcgc tctcggatcc cgccgggatc gtgcggttga ctgcggccga
     5461 ggccctcggt tccgccggcg atgagagggc gcttgagccg cttgagcgac tgaagttctc
     5521 ggacccggat ataggggtcc ggcgggctgc gtcgcttgcc cacgcccggg tggcggcaag
     5581 gcttgcggag aagaaggcgg tggaaggctg gctccttgat aggtaggccc gccagcacac
     5641 cggggagatg gggtcaactc ccgatcagga accggacaag gaacggctgc ccgtgccagg
     5701 atgtcctgac gtaccgcagg aagtacgcaa gcaggatcat gccggtatag agaaccagga
     5761 agacgcccag gtacgccggc aacggcaact gaagtccgaa gggctcgatg acttttacga
     5821 tgaccgcata ctgggcgagg tagatggcaa gcgagcaccc gccggccgcc cgcagggggt
     5881 caaggagccc gtactgcgcg gggatgtgat cgagaacggt cagtgcgaag acgacgagcc
     5941 cggcagcaaa cacgaagaaa ccgatcatcg gcgggtaaaa aagctccacg tagctgtcac
     6001 ggatgtattg tgcgccgggg aagagcatcc agaggacggc cccggcaagc aggaggccgg
     6061 caccccaccg tccctcgtcc ttgaaccgga accgcggtgc ggccccctca cgccaccgat
     6121 acctcccgag ctcggctccg aagaaggcga tcgcaagcca ggggaagacc gggaaccagc
     6181 cctccatgag ccaggcccgg gcgatctcga caggtgccgg gaacgggtcg ccggagatgg
     6241 agaagggggt gagcgtgacg tcaggtctcg tgatgaccgc ccccgaatag cccaacccca
     6301 cctggagaag aggtgttaga cagaggatcg cgatgatgac ggcccagcgt ccccgctccg
     6361 ggaggccgag atagaggtag gtgagcggaa gagcaaggcc gatgaggtag agcacatcga
     6421 cgtcaaggaa cgggacgtag tcaaagatca gcacctggag aagcgccccg gcgaggatga
     6481 agagcccacc cctcgtggcg atatacgaga agggcctgcc cttgccggtc cgtgagaggg
     6541 cgaccatcat accggcaaga ccgatgaaga tcggggctgc aagggaggag aggaaacggt
     6601 accagcccgg tgcagggggc aggaggagcg ccggcgtgag gtttgccatg atcatgaacg
     6661 tgatggcaag gccccggagt atgtcgacgg ggatatcacg aaaagggcca gctttggaga
     6721 ctggtttcgg aggcgggtac tccatatgtt gcgtgtgatc tttaagggat aaattttgct
     6781 gaatactctt gccctgagga ggggcgctcc ccgggagata acgttatccg gaccaggaat
     6841 catcctgtgc tcggggcagg ggacgtatcg cgcttcagcc cgtgcggccg gccgtgcatc
     6901 cagaacatcc cagggaagga atatatacac cggagacatg ccagccctga cgacattcgg
     6961 gagtctgtca tgactgcacc ggaagatatc gatgcaatgc gcgatgcccg ggacgtcgca
     7021 gggttgatcc gggccctcga cgatgccgac gagtttgtca gggcgcaggc ggccctctcg
     7081 ctcgggacga tcgccgaccc ggaggcccgg gagccactcg atcggatgcg aagagaggac
     7141 ccgagcgcct cggcccgcga ggctgccgcg accgcgtaca aatggctggt cggccgcctc
     7201 gaggaggtcg aggcggtcag gggacccaaa cccccgccgc cctggtagat gcggctcaca
     7261 ggacaaagaa gttgagccag aggatggcgg tgccgatccc gaccgtgacg aagagcacga
     7321 gcatccccac cttcaaaaac tctacaaagg agatagtgag accgttccgc gccgcgatcc
     7381 cgagcacgac gacgtttgcc gatgctccga tggccgtccc gttcccgccg aggcaggcgc
     7441 cgagcgagag cgcccaccag agcgggtagg tgttcatcga tgcgcccata tcgtggatga
     7501 gcgggatcag ggtggcggtg aggggtatgt tgtccacgac ggctgaggca attgccgaga
     7561 accaggcgat gatcagcatg gcctcgccct ccgagtggac gctctcgacc acgaacccgg
     7621 caacggccgc aatcgtcccg gtctcgacga gagcgccgac gacgatgaag agcccgccga
     7681 agaagaagag ggccggccac tcgatcttct caaagatctc ttccgggggc tgccggctcc
     7741 agaagaggag gatcgacgcg ccgatcaggg cgacgagggc gggttc
//
LOCUS       DAOSEE010000105         7758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_3469_length_7758_cov_185.403609, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000105 DAOSEE010000000
VERSION     DAOSEE010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7758
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3469_length_7758_cov_185.403609"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(737..1192)
                     /locus_tag="PLI76_08215"
     CDS             complement(737..1192)
                     /locus_tag="PLI76_08215"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOI62056.1"
                     /translation="MATIEDCFSKKLIRRVVPSREKANESLNLASSYLCEARQVAGIG
                     ANRMSLAGAYMAWFHAARAVLFRDGIREKSHYCIELYLETYVRSGDLEDEWVLMFGRI
                     RAQRHESQYSFGPTPTDAEVSAAVLHAERFIERMRQLLSETPKKEWVGR"
     gene            complement(1179..1736)
                     /locus_tag="PLI76_08220"
     CDS             complement(1179..1736)
                     /locus_tag="PLI76_08220"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOI62057.1"
                     /translation="MREAGLSELFKTEERIRILRYVAGQRTVTATAVVEATGTSKALV
                     SRYLHLLVREEFCTRHGRMYIWQENARSLATKRLLNIDLLRAQVPLPEWARGIGVYGS
                     YAEGTNTAESDIDLWVFVDEYTPKLEICAARIEKTVSVASGTEVHILILTPEKLAELR
                     EADTPFYAGLMRWGITIGGASIGND"
     gene            complement(1863..2792)
                     /locus_tag="PLI76_08225"
     CDS             complement(1863..2792)
                     /locus_tag="PLI76_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOI62058.1"
                     /translation="MPVISSVVNAVLLVIAGVILANILAETGIFSRLSSLTGPLCRFS
                     GLSDACVLSIVAMGVNATAGKSMLAEYYRDGKVTEREVIPVLIMGAFPVVLGESLFRV
                     QLPTALVLLGPVVGGVYTGLNLFSSGIQALFALIYSRRFLAGSGRAPPPVPSAAALML
                     NQEVVLAGCRKAVPTLRRVVPITLAALVVFSLLSATGVMDGIAAAFDPLLRAFGLPGE
                     SAAALVAQFLHFSAGYAIVASLLDGGVLTFETALVTLVIGSMVVITMIYVKYSVPLYL
                     SLFGRFGVRISLATYAASMAAKVITVALVFLLF"
     gene            complement(3029..3493)
                     /locus_tag="PLI76_08230"
     CDS             complement(3029..3493)
                     /locus_tag="PLI76_08230"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI62059.1"
                     /translation="MLPRKAGSIFLGKMFGVSTDVMLQSIIIFPSAFLIGAYTDLSVF
                     VQALPIMLLIAVGLTFSSLVKSFEGFGLIQTFINLPIFFLNGALFLLSNVPQWLLWIN
                     RSNPLTYGVDALRTVMMGGHAPLLPMYVNIGVLHASDAVMFTIGVYAFWGTE"
     gene            3648..4310
                     /locus_tag="PLI76_08235"
     CDS             3648..4310
                     /locus_tag="PLI76_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62060.1"
                     /translation="MAQRTYPGHTLIGNIATTKRRAKGDVMRQLKQERQADPGAESWL
                     LMFQGEYQPGYWITLLARKNATLKAIDTELRYVWLECCSHLSAFTIYGEEYSSYPDRW
                     TTEKGMNVPLGKLFSAGLTGKYIYDYGDSTYLTFKVLDLVPYAPAGGKDIELVARNDP
                     PDIRCSVCGKPATTICLECLDESPGDAFLCDDCFGEHECDEEMSLPVVNSPRMGQCAY
                     TG"
     gene            4803..5837
                     /locus_tag="PLI76_08240"
     CDS             4803..5837
                     /locus_tag="PLI76_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HOI62061.1"
                     /translation="MATTCRITDELTMNLKGWIERDGVRLSPADVERMLREEPAALAR
                     CGGEFLLEWDDCIARDTFGIMPGQIAPGTICCGGREAARIAPDPPPCGLEEAIVTAVG
                     LRSNEGAVAFSGGVDSALIAGLADLPCVAVGLRGSHDIARAAAAARMMGLDLAIVTPT
                     EEEVAEALREVVRVIPDPTNPIDASIAATLSFVAAWAGENGHTRILAGQGADELFGGY
                     ARYLSSPDLAAELERDVMDHGRQRARDQAVAALHGAYFSMPYLDTRVVRAARAIPAGE
                     KVRGGVRKHPLRTVAERHIPAEIARAEKKAMQYGSGIWRTMHQLTRQNGYKKSVQGYL
                     TEISRAEHGN"
     gene            5827..6096
                     /gene="gatC"
                     /locus_tag="PLI76_08245"
     CDS             5827..6096
                     /gene="gatC"
                     /locus_tag="PLI76_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOI62062.1"
                     /translation="MVTESDIEHIAELADIGISRDEVPEFTRQFNAILEYFEILDTVE
                     GESAPAGGITNAFRDDEPRPCLSQEAALANAGSTEDGFIKAPRVM"
     gene            6097..7398
                     /gene="gatA"
                     /locus_tag="PLI76_08250"
     CDS             6097..7398
                     /gene="gatA"
                     /locus_tag="PLI76_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOI62063.1"
                     /translation="MAGTLDFSPDDRYNAFITTCSTAPFSGGALAGIAVAVKDNISTA
                     GIETTCGSRILEGYIPPYDAYVVELLRREGAAIVGKTNMDEFGMGSTTETSAFGPTKN
                     PVDPSRVPGGSSGGSAAAVAAGLVPMALGSDTGGSIRCPAAFCGVVGLKPTYGRVSRY
                     GLIAYASSLEQVGPMARTVEDVSRVMSVIARYDPRDATMFDRPYDHRPSADIGGLRVG
                     IPGEYFGEGVDPRVAKKVRDAIGVIEELGASTVPVSIPGMRYALAAYYVICTAEASSN
                     IARFDGVRYGPAVDTRKSWHEAYQDIRQEKLGPEVRRRVMLGTFALSAGYAGRYYAKA
                     LGARQKIRQDFERAFRDVDVIAGPTMPTVAFPLGERTDPVSMYLSDILTAPANLAGIP
                     AISVPCGRVDGLPVGLQLMGRQFEEERIIDAAYAYEQGVKA"
     gene            7395..>7758
                     /locus_tag="PLI76_08255"
     CDS             7395..>7758
                     /locus_tag="PLI76_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HOI62064.1"
                     /translation="MKTIVGLEIHVQLATATKLFCGCSTDYRDDGPNTHCCPICLGLP
                     GSLPRLNRKAVEYGLLVAKALDMRVLEESDFARKNYFYPDLAKAYQITQSDRPLAVEG
                     TVEIEDDEGHEKIVRITRV"
BASE COUNT         1612 a         2448 c         2334 g         1364 t
ORIGIN      
        1 aaaaacgggc ggcctcaggt cggtttcgtg aaaatagtcc gggcggcccg gagcgtctca
       61 gcatcccagg gtatgacgac gcccgtttcc tttcagagca gaagaaacac ccgctcgcgg
      121 ccatgctcga agacgtaccg gtcgccccgt acccgaccgc cgaccgccat gcccgacctc
      181 acagggaatt gagggaaccc gcttccccga ttttttcccg ggacagacca gcgcgcgggg
      241 tcctgccgta cgccgctcga tcgcgaagct ccgtgcggcg aacgatgcgc ccgtagcaca
      301 ccattccgac aggataatca tgcaggagta caaacagaga aatggccctg tttcccttca
      361 accatctcca tgttaaggtt tacggggcca tctccctgcg ccgggatgag ttccgtcact
      421 tttcccggcg cagttacacg cctgcaagga tccgttgaac agagcgaacc gcatcgcccc
      481 cttcacccct ggcaagttag aacgcgctgt ggctgttcat ggtaatgcgc gatacctggt
      541 atgggcgccg gacctctcct ccctgttaag tcccggtact tccaggtctt tcaaccgacg
      601 gagaatcgcg gatttggaca tgcccagggc acgagcaacc tcgctgcttt tcgaggggtc
      661 gcggactgca ttcaaaggtg cttcgtaaga ccggctcagt ccgatgtgag cggcctctcg
      721 tgcatgaata atcgaatcat ctccccaccc attctttttt aggcgtttcg gagagcagct
      781 gcctcatcct ctcgataaat cgctcagcat gcagaacggc agccgagacc tccgcatcgg
      841 tcggcgtcgg cccgaatgag tactgacttt catggcgctg tgccctaatt ctgccgaaca
      901 tcagtaccca ctcatcttcg aggtctcccg atcgtacata cgtttcgaga tacaactcga
      961 tgcagtaatg gctcttctca cggataccgt cacggaagag tacggcacgg gcggcatgga
     1021 accacgccat gtacgctccc gccaggctca tccggttcgc accgatcccg gcgacctgcc
     1081 gtgcctcgca aagataggag gaggccaggt tcagtgactc gttcgccttc tctctggaag
     1141 gcacgaccct tcggatcaac tttttcgaga aacagtcctc aatcgttgcc aatggacgca
     1201 cccccgatcg ttatacccca cctcatgagg ccggcataga acggtgtatc agcctcccga
     1261 agttctgcta gcttctccgg ggtgaggatg aggatatgga cctcagtccc tgatgctaca
     1321 ctcacggttt tctcaatcct cgctgcacaa atctcaagtt tcggtgtgta ttcgtcgacg
     1381 aatacccaga gatcgatgtc gctttcggcc gtattcgtcc cttccgcata gctcccgtag
     1441 acgcctatcc cccgggccca ttcggggagc gggacctgcg cccggaggag atcgatgttg
     1501 agcagacgct ttgttgccag gcttctggca ttctcctgcc agatgtacat ccggccatgc
     1561 cgtgtacaga actcttccct gacaagcagg tgcaggtacc gtgagacgag agcttttgat
     1621 gtcccggtcg cttcgacaac cgccgtcgcc gttaccgtcc gctgccctgc cacgtaccgg
     1681 aggatgcgta tccgctcctc ggtcttgaac agttcgctta atcccgcctc tctcatgcag
     1741 tttctattcc gtgaacaaaa tgtttccatt gtagaaacaa aaagtttccg atccgggaac
     1801 atcttccgaa cagcccgaga tacttctcag tatcccgggg gtatgctgac ttccgttccc
     1861 cttcagaaca gaagaaacac cagcgcgacc gtgatcacct tcgcggccat gctcgctgca
     1921 tacgtggcga gcgaaatccg gacgccgaac cggccgaaga gcgagaggta gagcggcacg
     1981 gagtacttga cgtagatcat ggtgatgacc accatgctcc cgatgacgag cgtcacaagc
     2041 gccgtctcga acgtgagaac gcccccgtcg agcagggacg caacgatcgc atacccggcc
     2101 gagaagtgca ggaactgggc cacgagcgcc gcagcgctct cgccggggag gccgaaggcc
     2161 cggaggagcg gatcgaatgc ggcggcgatg ccatccatga cccccgttgc ggagaggagg
     2221 gagaacacga ccagcgcggc cagggtgatg ggaacgaccc gccggagcgt cgggaccgcc
     2281 ttcctgcacc cggcaagcac gacctcctga ttgagcataa gagcggccgc ggaagggacg
     2341 gggggaggcg cgcgcccgga gcccgcgagg aaccggcggc tgtagatgag ggcgaagagg
     2401 gcctggatgc cgcttgagaa gaggttcagc ccggtgtaga cccccccgac gacggggccg
     2461 aggaggacca gggccgtggg gagctggacg cggaagagcg actccccgag caccaccggg
     2521 aacgcaccca tgatgagaac cgggatcacc tcccgctccg tcaccttccc gtcacggtag
     2581 tattcggcaa gcatcgactt ccctgccgtg gcgtttaccc ccatcgcgac gatcgagagg
     2641 acacacgcat cggagaggcc ggagaaccgg cagagcggcc cggttaacga cgaaagccgg
     2701 gaaaagatcc cggtctcggc aaggatgttc gcaagtataa ccccggcgat gaccagcagg
     2761 acggcgttta ccacggagga gatcaccggc atgccggata ccccttgtac ctatccaccg
     2821 ttccctgcac ccccaaacgc ctctgttcag taatacaatc caccacaatg tatacaacaa
     2881 gtattacttt tctgcaatta atagatctcc attctgccgg ggccgttgcg gtgaccacgg
     2941 agatcgaccg tcaccggcac tcgtcggcgg gatgaattcg ggatcatggg tcgaggagcc
     3001 cccgaccaag gtcaggagta gttgatggtt attccgtccc ccaaaacgcg tataccccga
     3061 tggtgaacat cactgcatcg gaggcgtgca gtacgccgat gttgacgtac atcgggagca
     3121 ggggcgcgtg cccccccatc atgacggttc tgagtgcgtc gacaccgtac gtgagcgggt
     3181 tagagcggtt gatccacaac agccactgcg gcacgttcga cagcaaaaag agcgccccgt
     3241 tcaggaagaa gatcggcagg ttgatgaacg tctgaataag gccgaatcct tcaaaactct
     3301 tcaccaggct ggaaaacgtc aggccgacgg cgatcaggag catgatcggc aacgcctgta
     3361 caaagacgga aagatccgta tatgcaccga tcaggaaggc agacgggaag attataatgc
     3421 tctgcagcat tacatcggtg ctgacgccga acatcttccc aagaaagatg gatcctgctt
     3481 tccgcggtaa caactaccac tttctgaatt aatcagccga atcaggctaa cctcaggcca
     3541 gggcactata tttaaaacat cgagccaaac gatggcatcg aggttaatcc agccccgacg
     3601 ggatcgtaca acgcaacaat tttcactcaa tataccagag actcatcatg gcgcaacgca
     3661 cctatcccgg gcataccctc atcgggaata tcgcaaccac aaagcgaaga gctaagggcg
     3721 atgtcatgcg gcaactcaaa caggagcggc aggccgaccc gggagcagaa tcatggcttc
     3781 tcatgttcca gggagagtac caacccggat actggatcac ccttcttgca aggaagaatg
     3841 ccacgctgaa ggcgatagac accgaactca ggtatgtctg gctcgaatgc tgcagccacc
     3901 tctccgcctt cactatatac ggcgaggaat actcctccta cccggacaga tggaccacag
     3961 agaagggcat gaatgtcccc ctgggaaaac tcttctcggc gggcctgaca ggcaaataca
     4021 tctatgacta cggcgactcc acctatctca ccttcaaggt gctcgatctc gttccgtatg
     4081 ctccggcagg cgggaaagat atcgaactcg ttgcccgaaa cgatccgccc gacattcgct
     4141 gttccgtctg cgggaaaccg gcaaccacga tctgcctcga gtgtctcgat gagtctcccg
     4201 gtgacgcctt cctttgcgac gactgcttcg gcgagcatga gtgcgatgag gagatgtcgc
     4261 tccctgtcgt caactcgccg cgaatgggcc agtgcgcgta taccggatga ccacaacgag
     4321 gtctggtctg catctgccgc ggtcttcatg aggagagaga gcgtcgagta cagaaaccgt
     4381 gatgggcgct cttcacaggt ggccggcgcc cccctccgcc gccccgtgca agggcactta
     4441 ctctccgtcc tatcgccccg tccggttcga ggatgcggag accgcagagg agccggtgtg
     4501 aactcctgcc cgtgagcagt tctggcagca tagatctcgc aaaagagtca ggggagggcc
     4561 ggaccggacc cggaggggcg acgcgtgggc gtagtgcctt ccgtggcctc ctcccgggcg
     4621 ccggcacccc tccattcagg atcggggccg caaccctgca cccaatacgg gtgcttatcc
     4681 ggcggaaatc actgaaatac cgtgtttttc gcgccgaacc cggtgcgtat atttatagtt
     4741 taacagtatc aaatattgat actgatccgc ttttttgagg ggccgggtac tgcggcagcc
     4801 tcatggcaac cacctgccgg attacagacg agctgaccat gaacctgaag ggctggatag
     4861 aacgcgacgg cgtgcggctc tcgccggccg acgtggagcg gatgctccgc gaggagccgg
     4921 cggcccttgc ccggtgcggc ggcgagttcc tgcttgaatg ggacgactgc atcgcccgcg
     4981 acaccttcgg tatcatgccc ggccagatcg cgcccgggac catctgctgc ggcgggcggg
     5041 aggcggcccg gatagccccc gacccgccgc cctgcggcct cgaggaggcc atcgtcaccg
     5101 cggtaggcct ccggagcaac gaaggggcgg tggcgttctc cggcggcgtg gactcggccc
     5161 tcatcgccgg actcgccgat cttccctgcg tggcggtggg cctccgcggg tcgcacgaca
     5221 tcgctcgggc tgccgcggcc gcacggatga tggggctaga cctcgcaatc gtcacgccga
     5281 cggaggagga ggtcgcagag gctctccgcg aggtcgtccg ggtcatcccc gacccgacga
     5341 accccatcga cgcctcgatc gcagcgaccc tctccttcgt cgccgcatgg gccggcgaga
     5401 acggccacac ccggatcctc gccggtcagg gggcggacga gctcttcggc ggttacgccc
     5461 gctacctctc atcccccgac ctcgcggcgg aactcgagcg ggacgtcatg gaccacgggc
     5521 gccagagggc gcgggatcag gcggtggcgg cgctgcacgg ggcatacttc tcgatgccct
     5581 acctcgatac ccgggtggtg cgcgccgcac gggctatacc ggcaggcgag aaggtgcgcg
     5641 ggggggtgag aaagcatccc ctgcgaactg tcgcagaacg tcacattccc gccgaaatag
     5701 cccgtgcaga gaagaaagcg atgcagtacg gaagcggaat atggcggacg atgcaccagc
     5761 tcacccgtca aaacggttat aaaaagtcgg tacaagggta cttaacggag atcagcaggg
     5821 cagaacatgg taactgagag cgacatagag catatagcag agcttgcgga tatcggcata
     5881 tcacgagacg aggtgccgga gttcaccagg cagttcaacg cgatcctcga atacttcgag
     5941 atcctcgaca ccgtagaggg cgagagcgct ccggcgggcg ggataacgaa cgcctttaga
     6001 gatgacgagc cccggccctg cctctcgcag gaggcggccc tcgcgaacgc agggtcgacc
     6061 gaggacgggt tcatcaaagc accgagggtg atgtgagtgg cgggaactct cgacttttca
     6121 ccggacgacc ggtacaacgc cttcatcacc acctgcagca cagcgccgtt ctccggcggc
     6181 gcgctcgccg ggatcgccgt cgcggtcaag gataacatat cgacggcggg tatcgagacc
     6241 acctgtgggt cccggatcct tgagggctac ataccgccct acgacgccta cgtcgtcgaa
     6301 ctcctccgga gggagggtgc cgcaatcgtc ggcaagacca acatggacga gttcggcatg
     6361 ggctcgacga ccgagacgag cgcgttcggc cccaccaaaa acccggtcga cccctcgagg
     6421 gtccccggcg gttcgtccgg cggaagcgcc gccgcggttg ccgcgggcct cgtcccgatg
     6481 gcgctcggca gcgataccgg cggatcaatc cggtgtcctg cggcattctg cggtgtcgtg
     6541 gggctcaaac ccacctacgg ccgggtctcc cgctacggtc tcatcgccta cgccagctcc
     6601 ctcgaacagg tcgggccgat ggcgcggaca gtggaggacg tatcgagggt gatgtcggtg
     6661 atcgcacgct acgatccccg cgatgcgacg atgttcgacc gcccgtacga ccaccggccg
     6721 tcggccgata tcggggggct ccgtgtcggt ataccgggag agtacttcgg cgaaggcgtt
     6781 gacccccgcg tcgcaaagaa ggtccgcgac gccatcggtg tcatcgagga actcggtgcc
     6841 agcacggtgc cggtgagcat acccggcatg cggtatgccc ttgcggccta ctacgtcatc
     6901 tgcacagccg aagcctcctc gaacatcgca cggttcgacg gcgtccgcta cggcccggcg
     6961 gtcgataccc ggaagtcatg gcacgaggcc taccaggaca tccggcagga gaaactcggc
     7021 cccgaggtcc ggcgccgtgt catgctcggc acattcgccc tctcggccgg ctatgccggc
     7081 aggtactacg cgaaggccct gggggcccgg cagaagatcc ggcaggactt cgagcgggcc
     7141 ttccgcgacg tggacgtcat cgccggcccg acgatgccga ccgtggcctt ccccctcggc
     7201 gagaggaccg atccggtctc gatgtacctc tcggacatac tcacggcgcc ggcgaacctt
     7261 gccgggatac cggccatatc ggtcccgtgc ggcagggtgg acggcctccc cgtgggcctc
     7321 cagttgatgg gaagacagtt cgaggaggaa cgcatcatcg atgcggccta tgcctacgag
     7381 cagggggtga aggcatgaag acgatcgtcg ggcttgagat ccacgtccag ctcgcgacgg
     7441 cgacgaagct cttctgcggg tgctcgacgg actaccgcga cgacgggccc aacacccact
     7501 gctgcccgat ctgtctcggt ctgcccgggt cgctcccgcg cctgaacaga aaggcggtcg
     7561 agtacggcct cctggtagca aaagccctcg acatgcgggt gctggaagag tcggatttcg
     7621 cccggaagaa ctacttctac cccgatcttg caaaggccta ccagatcacc cagagcgata
     7681 ggccgcttgc ggtcgagggg accgtcgaga tcgaggacga cgaggggcac gagaagatcg
     7741 tccggatcac ccgggtgc
//
LOCUS       DAOSEE010000106         7715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_3491_length_7715_cov_255.132376, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000106 DAOSEE010000000
VERSION     DAOSEE010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3491_length_7715_cov_255.132376"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PLI76_08260"
     CDS             <1..350
                     /locus_tag="PLI76_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286613.1"
                     /note="catalyzes the reduction of
                     methylenetetrahydromethanopterin to
                     methyltetrahydromethanopterin with the oxidation of
                     coenzyme F420; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5,10-methylenetetrahydromethanopterin reductase"
                     /protein_id="HOI62065.1"
                     /translation="TAMSIDMNEKKARNAAKIVAAFIAAGSPPALLQRHGLDLANVAK
                     IKEALGRFDFKTVGGLVGDKEIDAFTIAGTPDVVKQKCEDLAKAGVTQIIFGSPLGPD
                     MTNSIRLLGKYIV"
     gene            506..1432
                     /locus_tag="PLI76_08265"
     CDS             506..1432
                     /locus_tag="PLI76_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HOI62066.1"
                     /translation="MFTIREVRTERGTFQYRRESLTGIRCRISPVRVERKIDTVPALP
                     LSREGCPFCPDSIGVATPTFEDGSRLRYGESVTFPNLYPYAERHIVTVITPDHATGRF
                     GVRCLADALSGAAEALSRSPGYASINWNYLPSAGASIVHPHLQGIADTEPTFLAGLYI
                     AAARRYLADHGRLYWDDLVEHERRSGRFLFEDEIFWSASPVPLGEREVRGVLPVSTLA
                     DIGPYIEPLAGGILRIVEFYRSLGTHAFNVSILFDAPGAGRGHRAFCSLIARLNPNSL
                     SMCDSAFMERLHLEPVILTLPEDLGALFREKG"
     gene            complement(1752..2594)
                     /locus_tag="PLI76_08270"
     CDS             complement(1752..2594)
                     /locus_tag="PLI76_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F420-dependent methylenetetrahydromethanopterin
                     dehydrogenase"
                     /protein_id="HOI62067.1"
                     /translation="MVVKVGIAKLGNIASGVMGELLLDERADREDMITFMATSGTKLQ
                     PEDIDRVVSNMKAWGPDFCIVVSPNGVLPGPTKAREDLAAAKIPCIVITDDVTTKKEQ
                     FDALKASNFGYIIMKADAMIGARREFLDPIEMADYNGNLVKVLAITGAFRKMQQELDK
                     VIDQVKAGKKGADLALPKVVMTVDRAVDGEFSNPYALAKARAAYEIAQSVAGVNVRGC
                     FMTKEWEKYIPMVSSAHEMMRQAMLLCEEAREIEKSTDGVIRKPHKKTGEIVSKTKLI
                     SKPE"
     gene            2736..3140
                     /locus_tag="PLI76_08275"
     CDS             2736..3140
                     /locus_tag="PLI76_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tfx family DNA-binding protein"
                     /protein_id="HOI62068.1"
                     /translation="MKQGLLTDRQKEVLRYRKRGLTQQQIADIIKTSKANVCTIEKAA
                     LENIARARETLEFLYTLDAAHLCTLEGGLDLLKAPEIIYAEAERMGVKVKYDTISLIN
                     RLRDANPDRFRGRQVRDDVEIYINQDGDLYFG"
     gene            3937..4950
                     /locus_tag="PLI76_08280"
     CDS             3937..4950
                     /locus_tag="PLI76_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOI62069.1"
                     /translation="MPKYNRPRRGSLAYSPRKRAKSPVPRYGSWPEYKGAPAVQGFAG
                     YKVGMTHVIMVDDHKSSPTEGRDVMVPVTVVEVPPMRVAGVRAYCEDTYGKHALTEAW
                     TTDLDDELSRRINVPKNHDTAAALDAIKAAIGEGRVTELYALAYTRPMELTGVPKKVP
                     DLMEMRIAGGSLDDQVTYAAGVLGKEVVISGNLKVGEYVDVTAVTTGKGTEGPVKRWG
                     VQVRKRKHSRGGKKRHIGNLGPWHPHHVRWQVPQMGQMGYQQRTEFNKRILKIGTNGD
                     EITPEGGFLHYGLVRGPYVLIKGSVPGPNKRLVRIRPAIRQGEHTVRTPTINFVSAQS
                     KQG"
     gene            4957..5706
                     /gene="rpl4p"
                     /locus_tag="PLI76_08285"
     CDS             4957..5706
                     /gene="rpl4p"
                     /locus_tag="PLI76_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOI62070.1"
                     /translation="MKAQVRTLTGEIAHEIDLPEIFNEEYRPDLIKRAVLALQSTRFQ
                     PHGTDPYAGLRTSAESWGSGRGVAQVPRLKNGSRVARVPQATGGRAAHPPKVAKVLVK
                     EINRKEKQKALRSAIAASTSPELVRGRGHLFEGDLPLVFEDRFEEVTRTADVIAALSA
                     LGVYADVERAKGSKKVRAGRGTMRGRRYKQRKSILIVTGDEPLRAARNLAGVDAVAVD
                     QLNAELFAPGTEAGRLTVWTESAIRRLEELS"
     gene            5703..5951
                     /locus_tag="PLI76_08290"
     CDS             5703..5951
                     /locus_tag="PLI76_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOI62071.1"
                     /translation="MILKYPFVTEKATMMLEGENKLQFIVQKNATRQEIKRELEAAFE
                     QDVVAIRTMMTMKGEKKAIVTFSDAKAAEEILSRLGIM"
     gene            5964..6683
                     /locus_tag="PLI76_08295"
     CDS             5964..6683
                     /locus_tag="PLI76_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOI62072.1"
                     /translation="MAHRIIAQNRGKGGPTYRAPSHRYKAELRHAGKNTTLTSGRVVD
                     IEHDPARHAPIALTRLDDGKKIYVLATEGLGVGDMVTWGSGGAVKNGNTLPLQEIPVG
                     AYICNIEARPNDGGKFVRASGVQALVIGKADDGRVGVRMPSGKNKWFSGVCMATVGIV
                     AGGGRGEKPFAKAGKKHFHVKSSAEKWPRVKGVCMNVIDHPFGGGGHQHCGRPKTVSR
                     GTSPGRKVGHIAARRTGKWKK"
     gene            6694..7107
                     /locus_tag="PLI76_08300"
     CDS             6694..7107
                     /locus_tag="PLI76_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOI62073.1"
                     /translation="MAKKTQKRMPRRREEFTYRGHSVTDLQQMALSELLPIMPARARR
                     KFDRGLSRDHEKLLADVRSGDETIRTHLRDMVIMPEMVGRTIQIHNGKEFQKVEIQPE
                     AVFHYLGEFALTRKRVSHGSAGIGATRSSKYVPLK"
     gene            7113..7574
                     /locus_tag="PLI76_08305"
     CDS             7113..7574
                     /locus_tag="PLI76_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOI62074.1"
                     /translation="MARTEYSANIEGENVARAKANELPISPKHSIEIARFIKNMTTTE
                     ARAYLADVVELKKAIPFKRFNRNVAHKRGLDRWAAGRYPVKAAEAYIRLLKSVEQNAE
                     YIGLDTENLRIDHVAANTGRGLRAFFPRAMGRATPKRRETVNIEIVVTEVA"
     gene            7574..>7715
                     /locus_tag="PLI76_08310"
     CDS             7574..>7715
                     /locus_tag="PLI76_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOI62075.1"
                     /translation="MAIEKKFITDGVRNVRVEKFLTKELKRAGYGGMDITRTPLGTQV
                     TIF"
BASE COUNT         1602 a         2427 c         2380 g         1306 t
ORIGIN      
        1 acaccgccat gtccatcgac atgaacgaga agaaggcccg gaacgccgca aagatcgtag
       61 ccgcattcat cgccgccggc tccccgcccg cgctcctgca gcgccacggc ctcgacctcg
      121 ccaacgtcgc gaagatcaag gaagcccttg gacgctttga cttcaagacc gtcggcggac
      181 tcgtcggcga caaggagatc gacgccttca ccatcgccgg aacccccgat gtcgtcaagc
      241 agaagtgcga agacctcgca aaggctggag ttacccagat catcttcggc tcgcccctcg
      301 gccccgacat gaccaactcc atccgcctgc tcggcaagta catcgtgtaa ggcagaaaca
      361 accctcacca ctttttcttg aacggttctg gccccgacga agggcacgcg caggcctcta
      421 tcgcgtacct cctccgcggt ctccgccacc gataaccctg acagggcttt atacgatagg
      481 cgcgccaatc ggagtacgaa gggtcatgtt caccatccgc gaggtcagga cggaacgagg
      541 aaccttccag taccgccggg agtccctcac cggcatccgg tgccggatca gccccgtccg
      601 ggtcgagcgt aagatcgata ccgttcccgc cctccctctc tctcgcgaag gctgcccgtt
      661 ctgccccgac tcgataggcg tcgccacccc gacgttcgag gacggcagcc gcctccggta
      721 cggggagagc gtgacgttcc cgaacctcta cccctacgcg gagcgccaca tagtcacggt
      781 gatcacgccc gatcacgcaa ccggccggtt cggcgtccgg tgcctcgccg acgccctatc
      841 cggcgcggca gaagccctat cccggtctcc cggctacgcg agcatcaact ggaactacct
      901 cccttcagcc ggtgcgagca tcgtccaccc gcacctgcag ggtatcgccg atacggaacc
      961 caccttcctt gcgggcctct acatcgccgc cgctcgccgg tacctcgccg atcacggtcg
     1021 cctctactgg gacgacctgg tggagcacga gcgccgctcc ggccggttcc tctttgagga
     1081 cgagatcttc tggtcggcaa gccccgtccc cctcggcgag cgggaggtgc ggggggttct
     1141 cccggtatcg acgcttgccg atatcgggcc ctacatcgag ccgctggccg gcgggatcct
     1201 ccggatcgtc gaattctacc ggagcctcgg gacgcacgcc ttcaacgtct cgatcctctt
     1261 tgacgccccc ggggcggggc ggggccaccg ggccttctgc tccctcatcg cgcggttaaa
     1321 cccgaacagc ctctccatgt gcgactcggc gttcatggag cggctgcacc tggaaccggt
     1381 catcctgacg ctccccgagg acctgggagc gctcttccgg gaaaagggtt gattctggcg
     1441 ttgagcctta acggtgggga ttccgcgccg ggatcctcga cgtccggttc ccctataccc
     1501 gggaggcggt cgaccgggtc cgtaccctga ccgtcggcga cccgcgcgac atccggacca
     1561 tcgcggagcc gacgatccct gaggctgcag cgcatgtgca aatgtgagag cgcgggtcgc
     1621 cgaaggcacc tgcgcggggc cgctcgcccc acaacccacc ccggcacggc gccggtggtt
     1681 cccggcaacc cggatggcgg ggcgcggtcc gatcacccac tccagggtcg aaaaaaggga
     1741 tggtgttggg tttactcggg cttgctgatg agttttgtct tcgagacgat ctcgccggtc
     1801 ttcttgtggg gcttcctgat gacgccgtcc gtcgacttct cgatctcgcg agcctcctcg
     1861 cagaggagca tggcctggcg catcatctcg tgggcgcttg agaccatcgg gatgtacttc
     1921 tcccactcct tcgtcatgaa gcagcccctg acgttgacgc cggcaaccga ctgcgcgatc
     1981 tcgtaggccg cgcgggcctt cgcgagcgcg taggggttgc tgaactcgcc atcgacggcc
     2041 ctgtcgacgg tcatgacgac cttcgggaga gcgaggtctg cgcccttctt gccggccttg
     2101 acctggtcga tgaccttgtc gagttcctgc tgcatcttcc ggaacgcgcc ggtgatggcg
     2161 aggaccttga cgaggttccc gttgtagtcc gccatctcga tggggtcgag gaattcacgc
     2221 cgggcgccga tcatggcgtc ggccttcatg atgatgtagc cgaagttgct cgccttgagc
     2281 gcgtcgaact gctccttctt ggtggtgacg tcgtcggtga tgacgatgca ggggattttg
     2341 gccgctgcga gatcttcacg ggccttggtg ggtccgggga gaacgccgtt cggggagacg
     2401 acgatacaga agtcggggcc ccatgccttc atgttgctga ccacgcggtc gatgtcctcg
     2461 ggctgcagct tcgtgccgga ggtcgccatg aaggtgatca tatcctcacg gtcggcacgt
     2521 tcgtcgagga ggagttcacc cattacaccg ctggcgatat ttcccagttt cgcaattcca
     2581 actttaacaa ccacttttaa cacctctcaa aatttgagaa cacgatagta tcaccaaatt
     2641 ctcatataaa cattttgaaa tggccccgga aaggtgcgac cgcgggggtg gttaaggaaa
     2701 agtgtttaaa tttgtcaagg tcatatataa ttctgatgaa acaaggtctg cttaccgatc
     2761 gccagaaaga agtgttgcgc taccggaaga ggggactgac gcagcagcag atcgcagaca
     2821 tcataaagac ctcaaaggcc aacgtctgta ccattgagaa ggcagccctt gagaacatag
     2881 cgcgtgcgcg cgagacgctt gagtttctct atacgctcga tgccgcacac ctctgtacgc
     2941 tcgagggggg cctcgatctg ctcaaagctc ctgagatcat atacgccgaa gccgagagga
     3001 tgggggtaaa agtcaagtac gacacgatat cgctcatcaa caggctcagg gatgcaaatc
     3061 ccgatcggtt caggggcagg caggtccgcg acgacgtcga gatctacatc aaccaggacg
     3121 gcgatctcta cttcggatga agcgaatcgg gggcgcgcac acgcgcgggc ctcgtgcggg
     3181 aatggccctg ccggagcgag tgctccgcac aaacggatct tttgcctctt ctccggcagc
     3241 agcatccttg cagcccttca ttcacccatc cggccgttcg gtccggggtg ccggcctcat
     3301 gcctgcagca gcacccgttc ggcgcaattt tgtgagagaa cggatgacaa ggtttatatc
     3361 ccattttaag taatgtttaa gaaaccgccc tgtataatcc tatccgggcc cccggggtag
     3421 ccggcacgtt atgtgccctc gatctccgga atctctctga tccgtaagat tctcggacct
     3481 gagacccgcg gcaggagagt acaggaggta ccaaacagac gaaggacgaa ccctccgcaa
     3541 ggagggccga tctcgattga ggagttgtgc tcgtcacaac gggatgacag gagctggatc
     3601 tgatcgagac gcggggccaa cgccagtgca gcgcgcccgg ttccggagac tctctccgga
     3661 agcgtcaaag agtcaggatc tattggcgac gactggagcg tacccgggtg gcacacccgg
     3721 gccggcaacg gagagcgatg ggatacgaag caggagagcg atctctcccg cccctcacgc
     3781 tctgttgcct ccatcaactg ttacccttca cgcccgggta tatctgcccg ggtgctcgat
     3841 cttctgcccg tcatgggttc gccggaagac cgccctgcgg catgcctgca gagcggcgca
     3901 tcagactaca acgcacgaag aagggttgat tgaaacatgc cgaagtataa caggccacgc
     3961 agaggatccc tcgcgtacag cccgcgaaaa cgggcaaaga gcccggttcc ccggtatggc
     4021 tcatggccgg agtataaggg agccccggcc gtccaggggt ttgcaggcta caaggtggga
     4081 atgacccatg tcatcatggt ggatgaccac aagagcagcc ctaccgaagg ccgggacgtg
     4141 atggtgccgg tgaccgtggt tgaagttccg cccatgcggg tggcaggcgt gcgcgcatac
     4201 tgcgaggaca cctacgggaa gcatgcgctg accgaggcct ggacgaccga tctcgacgac
     4261 gagttatcgc ggcggatcaa cgtcccgaag aaccacgaca ccgccgccgc cctcgacgcc
     4321 atcaaggccg ccatcggcga gggcagggtc acggaactct acgctctggc ctacacgcgg
     4381 cccatggagc ttaccggcgt cccgaagaag gttcccgacc tgatggagat gcggatcgcc
     4441 ggcggaagcc tcgacgacca ggtcacctat gccgccgggg ttctcgggaa agaggttgtc
     4501 atctccggga acctcaaggt cggcgagtac gtcgacgtga ccgccgtcac caccggtaag
     4561 ggcaccgaag gccccgtaaa gcgctggggc gtccaggtcc ggaagcggaa acactcccgg
     4621 ggcggcaaga agcgccacat cggcaacctc ggcccgtggc acccgcacca cgtccggtgg
     4681 caggtgcccc agatgggtca gatgggctac cagcagcgca ccgagttcaa caagcggatc
     4741 ttgaagatcg gcacgaacgg cgacgagata accccggaag gcgggttcct ccactacggc
     4801 ctcgtgcgcg gcccctacgt gctgatcaag ggttccgttc ccgggccgaa caagcgtctt
     4861 gtccggatac ggcccgctat acggcagggt gaacacaccg tccgcacgcc gacgatcaac
     4921 tttgtcagcg cgcagagcaa gcaggggtga gcagcgatga aggcacaggt tcgaacactg
     4981 acaggcgaga tcgcccacga gatcgatctc ccggaaatat tcaatgaaga gtacaggccc
     5041 gacctgatta agcgggcagt cctcgcgctc cagagcaccc ggttccagcc gcacgggaca
     5101 gacccctacg cgggcctccg cacctcggca gagtcctggg gcagcgggcg aggcgtcgct
     5161 caggtccccc gcctgaagaa cggaagccgg gtggcccggg ttccgcaggc aaccggcggg
     5221 cgtgcagccc atcccccgaa ggtcgcgaag gttctcgtca aagagatcaa cagaaaagag
     5281 aagcagaagg ctctccgatc tgcaatcgca gccagcacat ccccggaact cgtccgtggg
     5341 cgcgggcacc tcttcgaggg cgacctcccg ctggtctttg aggaccggtt cgaggaagtc
     5401 acccggacgg ccgacgtcat cgcggcgctc tccgcactcg gcgtctacgc cgacgtcgag
     5461 cgggcgaagg gcagcaagaa ggttcgcgcg gggcgcggca ccatgcgcgg ccgccgctac
     5521 aagcagcgca agagcatcct gatcgtcacc ggagacgaac cgctccgggc ggcccggaac
     5581 cttgccggcg tcgacgccgt tgcggtcgac cagctcaacg ccgagctctt cgcacccggc
     5641 acggaggcag gacgcctgac ggtctggacg gagtctgcaa tcaggaggct ggaggagctc
     5701 tcatgatact gaaatacccg ttcgttactg aaaaggcaac gatgatgctc gagggcgaga
     5761 acaaactcca gtttatcgtg cagaagaacg ccaccaggca ggagatcaag cgcgagctgg
     5821 aggcggcttt cgagcaggat gtggtcgcta tccgcaccat gatgacgatg aaaggcgaga
     5881 agaaggccat tgtaacgttt tcagatgcaa aggcggctga agagatcctc agccggctgg
     5941 ggataatgta aggtgagtag cgaatggcac atagaattat agcgcagaat cgcggtaaag
     6001 ggggtccgac ctatcgtgca ccgtcgcacc ggtacaaggc cgagctccgg catgccggga
     6061 agaacaccac cctgacatcc gggcgcgtcg tcgatatcga gcacgaccct gcccgccacg
     6121 cgccgatcgc cctcaccagg cttgacgacg gcaaaaagat ctatgtcctt gccaccgaag
     6181 ggcttggtgt cggggatatg gtcacctggg ggtcgggagg cgccgtgaag aacggaaaca
     6241 ccctgcctct ccaggagatc ccggtcggtg catacatctg caacatcgaa gcacggccca
     6301 acgacggcgg caagttcgtc cgcgcaagcg gcgtccaggc cctcgtcatc ggcaaggccg
     6361 acgacggcag agtcggcgtc cggatgccga gcggcaagaa caagtggttc agcggtgtct
     6421 gcatggcaac cgtcggtatc gttgccggcg gcggacgggg cgaaaaaccg ttcgccaagg
     6481 caggaaagaa gcacttccat gtcaaatcct ccgcagagaa gtggccccgc gtcaagggtg
     6541 tctgcatgaa cgtcatcgac cacccgttcg gcggcggcgg tcaccagcac tgcgggcggc
     6601 caaagaccgt atcccgcggg acatccccgg gaagaaaggt agggcatatc gccgcccgga
     6661 gaaccggcaa gtggaagaag tgaggggtga atcatggcaa agaagacaca gaagcgaatg
     6721 ccgcgacggc gtgaggagtt cacctatcgc ggccactccg ttacggacct gcagcagatg
     6781 gctctctccg agctgctgcc gatcatgccg gcccgggcac gcaggaagtt tgaccggggt
     6841 ctctcccggg atcacgagaa actcctcgcc gatgtccggt cgggggatga gaccatccgc
     6901 acccacctgc gtgacatggt catcatgccc gagatggtcg ggaggacaat ccagatccac
     6961 aacgggaaag agttccagaa ggtggagatc cagcccgagg cggtctttca ctacctcgga
     7021 gagtttgcac tgacccgtaa gagggtctct cacggcagcg ccggtatcgg tgcgacccgg
     7081 tcgagcaagt acgtaccgct gaagtgatgg ctatggcaag gactgaatac tcagcaaata
     7141 tcgagggtga gaacgtcgct cgcgcaaagg cgaacgaact tcccatctca cccaaacact
     7201 cgatcgagat tgccaggttc atcaagaaca tgaccaccac cgaagccagg gcataccttg
     7261 ccgatgtggt ggagctgaag aaggccatcc ccttcaagcg gttcaaccgg aacgtcgccc
     7321 acaagcgggg cctcgaccgg tgggcggcag gccgctaccc ggtcaaagcc gcggaagcct
     7381 acatcaggct gcttaagtcc gtcgagcaga acgccgagta catcggcctc gataccgaga
     7441 acctccggat cgaccacgtc gccgcgaaca cgggccgtgg tctccgggcg ttcttcccgc
     7501 gtgcgatggg tcgcgccacc ccgaagcgca gagagaccgt gaacatcgag atcgtcgtga
     7561 ccgaggtggc gtaatggcaa tcgagaagaa gtttatcaca gacggcgtga ggaacgtccg
     7621 cgtcgagaag ttcctcacga aggaactcaa gcgggccggc tacggcggca tggatatcac
     7681 ccggacgccg ctcggcaccc aggtgaccat ctttg
//
LOCUS       DAOSEE010000107         7621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_3542_length_7621_cov_285.273725, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000107 DAOSEE010000000
VERSION     DAOSEE010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7621
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3542_length_7621_cov_285.273725"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..682)
                     /locus_tag="PLI76_08315"
     CDS             complement(212..682)
                     /locus_tag="PLI76_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62076.1"
                     /translation="MDESTEPGTPSPNEHQARYIRSTCEYIDRLLTDIEGVLDASTSG
                     SVFPRYSADLTPVQRETIEDFVADLRARLVRILDDQGIARDRPVVPASHAIKGSLIAI
                     DIAAEELRPKHVRAYGKVSGGMAAELEGIAGELHDLLGRLDRDLGEAAGDGREE"
     gene            751..1515
                     /locus_tag="PLI76_08320"
     CDS             751..1515
                     /locus_tag="PLI76_08320"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HOI62077.1"
                     /translation="MTIQVMGIHGLPIIHAGDDLAALICERTAFEDGDILCIASSVSA
                     KANGQTRNLTEIEPTERAKAIAAAAGEDPRFIQVILDTSVDVLLESPFILAAVPCGHI
                     GVRAGVDRSNVEDDTAIILPPDPMAAAAGIREDIRRITGRSVAVILTDTCGRAFRRGQ
                     IGHAVGWSGVPAIRDFRGDTDLFARPLTITEEAVVDEIAGFANIVMGESNNGVPAVVV
                     RGLPAWQGHDRLYFRPEEDVMRRALQTSRQNPESAP"
     gene            complement(1504..1668)
                     /locus_tag="PLI76_08325"
     CDS             complement(1504..1668)
                     /locus_tag="PLI76_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62078.1"
                     /translation="MAACPCCGAELTGYERVAVSGDKEKIRTETVLCCPCCQWQGICR
                     ENCPDDLLWG"
     gene            complement(1676..3439)
                     /locus_tag="PLI76_08330"
     CDS             complement(1676..3439)
                     /locus_tag="PLI76_08330"
                     /inference="COORDINATES: protein motif:HMM:NF012570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HOI62079.1"
                     /translation="MTSHARHLPEILACEADRVQSFGSEYASYADQLRELARRLDSGR
                     FHLAVLGQFKRGKSTLINALLGESLLPSSVIPLTAIPTLIAYGDRQSIRIRFQGNRDD
                     EVVEGGTPHDLYRSLLHSVSEEFNPKNERGVLQVEVTHPAPLLAEGVVLIDTPGIGSI
                     HQHNTEMTLNFLAQCDAALFLVSADPPITEVEVEFLRTIRDAVTKVFFVLNKVDYLTD
                     DEQKTALAFFETVLRERAGIQGPVTIFPVSAKRGLAAATAEDAALWQSSGMAKVSDHL
                     VGFLAGEKNAVLRDAVQKKARAVLGAVALRLDLSRRALEMPLDDLEGRMRTFETKIRE
                     AELQRRHAADILEGDHRRVAISLEEEMRALRERSLAHLMGVAEGALVGPAGPDETAAQ
                     NAVAAAIPSFYEHELGETTAAVEREVTALLQTHQQRAADLVGSIRTAAADLFEIPYHA
                     RENDRMLSMRQEPYWVSRKGWESTFSPVTNGLVDRALPRSMRERRIRARLEKQISYLV
                     VRNTENLRWATIQNVDATFRQFGRELDADLAEAVDATEGAITAAYTKRKEHASETAGD
                     LARLQKASAEVASAISDLEQG"
     regulatory      complement(3487..3548)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            3674..5002
                     /gene="glnA"
                     /locus_tag="PLI76_08335"
     CDS             3674..5002
                     /gene="glnA"
                     /locus_tag="PLI76_08335"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HOI62080.1"
                     /translation="MSDPAISTILNRIEQDNVRFLRLQFTDVLGMPKNVAIPAGQAEK
                     ALTTGIGFDGSSIEGFARIEESDMLLKPDPSTYTLLPWRPHEDAVARFVCDVTGPDGR
                     PFAGDPRYVLRRAMDDAAKDGYVFNTGPELEFFLFRMLDGRPTTQFQDVGGYFDLAPT
                     DLAEDVRREIIIALTEMGFEIEASHHEVAESQHEIDFKYSDALHTADSVITFKFAVKT
                     MALMRGLHASFMAKPIYGICGSGMHTNCSLAKDGKNAFYDPDAPRQLSETCMHFIAGL
                     LRHAPAITRVANPTVNSYKRLVPGYEAPCYVSWSASNRSALIRVPAPRGNSTRVEFRS
                     PDPTCNPYLAFAALLAAGMDGVRNRIEPPTGVGKNIYHMDAAERQDAGIRTLPADLAE
                     AHQALLADEIICRALGPHVVAALTNVANAEWDAYRTAVHPWELDRYLPTY"
     gene            complement(5076..6275)
                     /locus_tag="PLI76_08340"
     CDS             complement(5076..6275)
                     /locus_tag="PLI76_08340"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI62081.1"
                     /translation="MGDDGDGWLNRTVIGAGVTSFFSDLGHEAVTVLLPSLLILIGAP
                     AYVLGVIEGLSDGASSFVKLVSGHWSDRLGKRKEFALAGYLATAVFPAFVAVATVWPV
                     VLLGRVIGWAGRGTRGPPRDAILAKSVPPHDLGKAFGFHRAGDTLGAIAGPLMALVLV
                     TSIGLREILWLTLIPGFLAFATFWLLVREKNPTPSRDKTKFVASLRGLPGVFKRFLGA
                     VLVFGIADFSHTLLIAFAIVALTPSLGLTGATAAGAGLYLVRNVVYAAACYPFGVLGD
                     RFGRWNVLILGYAVAAATFIGFAVAPPDLIGYGALFALAGLFIAAEDTLEGAVAAELV
                     EEQRRGLGFGALATVNGIGDLVSSIAIGFLWAFIGYTAGFLFAAIVAAAGTLLLAAPW
                     LRPSAAA"
     gene            complement(6345..6995)
                     /locus_tag="PLI76_08345"
     CDS             complement(6345..6995)
                     /locus_tag="PLI76_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HOI62082.1"
                     /translation="MSGVDRWFSVAGLATGLIRPGDAIADRLVAAAPRPGGMRGGDCI
                     VVAESAVATAEGRLVRLDGVKPTPAARDLARRYTMDPRMVEIILQESDAVVGGMPGFL
                     LCRAHDTLLPNAGVDASNAPPGCVVLLPADPGASAAGIRDAIRASAGVEVAVIVADSR
                     PHPMRSGCAGIALGCAGLAAVMDGRAVADNIASAAELVMGEAGEGIPAALVRWCER"
     gene            complement(6992..7171)
                     /locus_tag="PLI76_08350"
     CDS             complement(6992..7171)
                     /locus_tag="PLI76_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62083.1"
                     /translation="MASMIEDLKRAVMGRLERARAAIAAGGDPEEELARATADIEALC
                     DGTDRLYRDAVCSMW"
     gene            complement(7179..>7621)
                     /locus_tag="PLI76_08355"
     CDS             complement(7179..>7621)
                     /locus_tag="PLI76_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOI62084.1"
                     /translation="PIVVIEDGLAEDDWDGWRLLTEAIGDRVELVGDDLFVTNVTRIE
                     RGIREGVANAVLIKPNQIGTVTETIAAVRLAQQNGWGAMVSHRSGETVDTFIADFTVA
                     MRTGHLKTGAPARGERVEKYNQLLRIEEAAGEAARYAGRAGFVR"
BASE COUNT         1306 a         2637 c         2432 g         1246 t
ORIGIN      
        1 tttagggtcc agtcttgaag aagttcaagg gttcgaatcc cttcccccgc atacccacag
       61 ttttcaacgt gtctgccgac caggtgcttg ctcccgccgt tttaccggct gtggccgccc
      121 atcacatggc tatcgccagg ggtcgtccca cttccgggaa taataccttc gagaagagaa
      181 gtgttccctc ccacccgggc gccggcaggc atcactcttc cctgccgtca cccgctgctt
      241 cgccgagatc ccggtcgagc cgcccgagaa ggtcgtgcag ctccccggcg ataccttcga
      301 gttccgccgc catccccccg gagaccttgc catacgcccg cacgtgcttc ggccgcagct
      361 cttccgccgc aatatcgatc gcgatgagcg aacccttgat ggcgtgcgac gccgggacga
      421 ccggccggtc ccgggcgatg ccctggtcgt ccaggatgcg gacgagccgg gcacgaagat
      481 cggcgacaaa atcctcgatc gtttcccgct gcacgggcgt gagatcggcg gagtagcgcg
      541 ggaagacgct tcccgacgtc gaagcgtcca gaacaccctc gatatcggtc aggagccggt
      601 cgatatactc gcaggtgctc cggatatacc gggcctggtg ttcgttcggg gacggcgtcc
      661 ccggttcggt gctctcatcc attctcgtcc ctctgctacg tgctacggtc tcccgcgacc
      721 cttatcacca ccggcgtcga ctacgtgacg atgacaatcc aggtcatggg cattcacggc
      781 cttcccatca tccacgccgg cgacgatctt gccgcactca tctgcgaacg gaccgcgttc
      841 gaggacggcg acatcctctg catcgcctcg tcggtctcgg cgaaagcgaa cggacagacc
      901 cgcaacctca cagagatcga gccgaccgaa cgggcgaaag ccatcgcggc agccgccggc
      961 gaggatccgc ggttcatcca ggtcatcctc gacacctccg tcgacgtgct ccttgagagc
     1021 ccgttcatcc tcgcggccgt cccctgcggg catatcggcg tccgggcagg cgtcgaccgg
     1081 agcaacgtgg aggacgatac cgccatcatc ctgccgcccg acccgatggc cgccgccgcc
     1141 gggatccggg aggatatccg caggatcacc ggcagatcgg tcgcggtcat cctcaccgac
     1201 acctgcggac gggcgttccg ccgggggcag atcggccacg ccgtcggctg gagcggggtg
     1261 ccggcgatcc gcgacttccg gggcgataca gacctcttcg cccgccccct cactatcacc
     1321 gaggaggcgg tcgtcgatga gatcgccggg ttcgcgaaca tcgtcatggg ggagagcaac
     1381 aacggtgtcc cggccgtcgt cgtccggggt cttcccgcct ggcaggggca cgaccggctc
     1441 tacttccgcc ccgaagagga cgtcatgcgg cgggcgctgc aaacgtcgcg acagaacccg
     1501 gaatcagccc cataggagat cgtcgggaca gttctcccgg catatcccct gccactggca
     1561 gcacgggcag cagaggacgg tctcggtccg gatcttctcc ttatctccag agacggcgac
     1621 ccgctcgtag ccggtcagtt ccgccccgca gcatggacat gcagccatct tctcttcacc
     1681 cctgctcgag atccgagatc gcggacgcaa cctctgccga cgccttctgc agccgggcga
     1741 ggtcgcccgc cgtctcgctc gcgtgctcct tcctctttgt gtaggcggcg gtgatggcac
     1801 cttctgtggc gtcgaccgcc tctgcgagat ccgcatccag ttcgcggccg aactgccgga
     1861 acgtcgcgtc gacgttctgg atcgtcgccc accggagatt ctcggtgttc cggaccacaa
     1921 ggtaggagat ctgcttctcc agccgcgccc ggatccgcct ctcccgcatc gagcgcggca
     1981 gcgcccggtc gacgagaccg ttcgtgaccg ggctgaaggt gctctcccag cccttccggg
     2041 agacccagta cggctcctgc cgcatcgaga gcatccggtc gttctcccgg gcgtggtagg
     2101 ggatctcgaa gaggtccgcg gcggcggtcc ggatggaccc gacgaggtcg gcggcccgct
     2161 gctggtgggt ctgcaggagc gcagtcacct cccgttcgac cgccgcggtc gtttcgccga
     2221 gttcgtgctc gtagaacgac gggatggcgg cggcaacggc gttctgcgcc gccgtctcgt
     2281 ccggccccgc cgggccgaca agcgcgccct ccgcgacccc catgaggtgc gcgagcgacc
     2341 gctcccggag cgcccgcatc tcctcttcga gcgagattgc taccctcctg tggtcgcctt
     2401 ccaggatatc ggcggcatgc cggcgctgga gttccgcctc gcggatcttt gtctcgaacg
     2461 tccgcatccg gccttcgagg tcgtcaagcg gcatctccag cgcccgccgg gagaggtcga
     2521 gccggagcgc gacggcgccc agcacggccc gggccttctt ctggaccgca tcgcggagca
     2581 cggcgttctt ctcgccggcg aggaacccga ccaggtggtc cgagaccttc gccatcccgc
     2641 tcgactgcca gagggcggca tcctcggccg ttgcggccgc aagcccccgt ttcgccgaga
     2701 ccgggaagat cgtgaccggc ccctggatac ccgcccgctc gcggagcacc gtctcgaaga
     2761 acgcgagcgc ggtcttttgc tcgtcgtcgg tgaggtagtc gaccttgttg aggacgaaga
     2821 agaccttcgt gaccgcgtcg cggatggtcc ggaggaactc cacctcgacc tcggtgatcg
     2881 gcgggtcggc ggagaccagg aagagcgccg catcgcactg ggcgaggaag tttaaggtca
     2941 tctcggtgtt gtgctggtgg atggacccga tcccgggcgt gtcgatcagg acgacaccct
     3001 ctgcgaggag cggggcgggg tgggtcacct ccacctgcaa cacgccccgc tcattcttcg
     3061 gattgaactc ctccgagacc gagtggagga gcgagcggta caggtcgtgg ggtgtgccgc
     3121 cctcgacaac ctcgtcgtcg cggttgccct ggaaccggat acggatgctc tgcctgtcgc
     3181 cgtaggcgat cagcgtgggg atggcggtca gcgggatcac cgagctcggg aggagtgact
     3241 cgccaaggag cgcgttgatc agcgtgctct tgccccgctt gaactggccg agcacggcga
     3301 ggtggaaccg gccggaatca agccggcgag ccagttcccg gagctggtcg gcgtaggaag
     3361 catactcgga accgaacgat tgaacacggt cggcttcgca ggcaaggatt tcgggcaggt
     3421 gccgggcgtg gctggtcatg ggatctctcc tgacaaatgg atatggatcg ggcgggcact
     3481 ggccgcacga tggtaggagt tatcagccac aaaagcggtt agaggtgaac tccatcacct
     3541 gaatctgtat ccctgtatct tgctctgcag ataattaatg ctttgttctc cgatagcctc
     3601 gctctcctga tgcgttctgc ctccccggta ccaggcaaga ttatatgtag agagagcagg
     3661 aacctttctc ccaatgtcgg atccagccat ttcaacaatt ctcaatagaa tcgaacagga
     3721 taacgtgcga tttttacgcc tccagttcac cgatgttctc ggcatgccca agaacgtcgc
     3781 catccccgcc ggccaggcag agaaagccct gaccaccggc atcgggtttg acggatcctc
     3841 gatcgagggg ttcgcccgga tcgaagagtc cgacatgctc ctcaaaccgg acccctccac
     3901 ctacaccctt ctcccgtggc ggccgcacga ggatgcagtc gcccggttcg tctgcgacgt
     3961 gaccgggccc gacggccggc ccttcgcggg cgacccgcgc tacgtgctgc ggcgggcgat
     4021 ggacgacgcg gcaaaggacg gctacgtctt caacaccggg ccggaactgg agttcttcct
     4081 cttccggatg ctcgacggcc ggccgacgac gcagttccag gacgtcggcg gctacttcga
     4141 cctcgccccc accgaccttg ccgaggacgt ccggcgcgag atcatcattg ccctcacgga
     4201 gatggggttc gagatcgagg cgtcccacca cgaggtcgcc gagagccagc acgagatcga
     4261 cttcaagtac agcgacgccc tccacaccgc cgacagcgtc atcaccttca agttcgcggt
     4321 caagacgatg gcgctgatgc gcggcctgca cgcctcgttc atggcaaagc cgatctacgg
     4381 catctgcggg agcggaatgc acacgaactg ctcgctcgcc aaagacggga agaacgcctt
     4441 ttacgatccg gacgctccgc ggcaactctc ggagacctgc atgcacttca tcgccgggct
     4501 gctccgccac gcgccggcga tcacccgggt cgcgaatccg acggtcaact cctacaaacg
     4561 gctcgtcccc ggctacgagg cgccctgcta cgtcagctgg agcgcgagca accggtcggc
     4621 cctgatccgg gtcccggcac cccgcggcaa cagcacccgg gtcgagttcc gcagccccga
     4681 cccgacctgc aacccctacc ttgccttcgc cgcgctcctt gccgccggga tggacggcgt
     4741 caggaaccgg atcgagcctc cgaccggcgt tggaaagaac atctatcata tggatgccgc
     4801 tgagcggcag gatgcgggga ttcggacgct cccggcagat ctcgccgagg cacaccaggc
     4861 cctgcttgcg gacgagatca tctgcagagc gctcggcccc catgtcgtcg cagccctcac
     4921 gaacgtcgcg aacgccgagt gggacgccta ccgcacagcg gtccacccct gggagctcga
     4981 tcggtacctc ccgacctact gatctctctt tttcatagcg tgccaggtat cgtgcatgaa
     5041 ccgggcacgc cccggtatgc tcaacgagtg tccccttatg cggccgcaga aggccggagc
     5101 catggtgcgg ccaggagcag ggttccggca gcggcgacga tcgcagcgaa caggaacccg
     5161 gcggtatatc cgataaacgc ccagagaaat ccgattgcga tactggatac caggtcgccg
     5221 atgccgttga cggtggcgag tgctccgaat ccgagacccc ggcgctgctc ttccacgagt
     5281 tctgccgcga ccgcaccttc aagggtatcc tctgcggcga taaacaggcc ggcaagagca
     5341 aagagtgcac catagccgat caggtccggc ggagccacgg cgaacccgat gaacgtcgcg
     5401 gctgcaaccg cgtacccgag gataagcacg ttccagcgtc cgaaccggtc gccgaggacg
     5461 ccgaaaggat aacaggccgc cgcgtagacc acgttcctga cgaggtagag gccggcgcct
     5521 gctgcggtcg cgcccgttaa cccgagcgaa ggggtcaggg caacgatcgc aaaggcgatg
     5581 aggagggtgt gcgagaagtc ggcgatcccg aagaccagga cagcgccgag gaagcgtttg
     5641 aagacccccg gaaggccccg gagcgaagcg acgaacttcg ttttgtcgcg ggacggcgtc
     5701 gggttcttct cccggacgag gagccagaag gtcgcgaagg caagaaaccc cgggatgagg
     5761 gtgagccaga ggatctcccg caggccgatc gaggtcacca ggaccagcgc catgagcggg
     5821 ccggcgatgg ccccgagcgt atcgccggcc cggtggaacc cgaaggcctt gccgaggtcg
     5881 tgcggcggga cggactttgc caggatcgcg tcgcggggcg gcccacgggt gccgcgtccc
     5941 gcccacccga tcacgcggcc aaggaggacg accggccaga cggtcgcaac cgcaacaaag
     6001 gcagggaaga ctgcggtggc gagatatccg gcgagcgcga actctttcct tttgccgagc
     6061 cggtccgacc agtggccgga gacgagcttg acgaagctcg aagcaccgtc gctcaacccc
     6121 tcgatcacgc cgagcacata ggcgggagcg ccgatgagga tcaggaggga agggagcagg
     6181 accgtgacgg cttcgtggcc caggtcggag aagaaactcg ttacgcctgc gccgatcacc
     6241 gtcctgttca gccagccgtc accgtcatca cccatgccct cctcctcttg cgggacccgc
     6301 gcccgccggg tcgcacgttc atttccctgc caccacagtc cagctcaccg ttcgcaccac
     6361 cggacgagcg ccgccgggat gccctcaccg gcctcgccca tgacgagttc cgcggcggat
     6421 gcaatattgt ccgccaccgc ccggccgtcc atgaccgccg cgagcccggc gcagccgaga
     6481 gcgatgccgg cacagcccga ccgcatggga tgagggcggc tgtcggcgac gatgacggcg
     6541 acctcaaccc cggcagacgc ccggatggcg tcccggatcc cggcggcgct cgctcccggg
     6601 tcggcgggga ggaggaccac gcaccccggc ggggcgttgg acgcgtccac gccggcgttg
     6661 ggcagaaggg tatcgtgagc ccggcagagg aggaaccccg gcatgccgcc gacgacggca
     6721 tcgctctcct gcaggatgat ctcgaccatc cgcgggtcca tcgtgtaccg gcgggcgagg
     6781 tcgcgggctg ccggggtggg tttaacgccg tcgagccgga caagcctgcc ttcggcggtg
     6841 gccaccgccg attcggcgac gacgatgcag tctccgccgc gcatgccgcc cggccggggc
     6901 gccgccgcga cgagccggtc ggcgatcgca tcccccggcc ggatcaggcc ggtcgcgagg
     6961 ccggcaacgg agaaccaccg gtccacgccg ctcaccacat cgagcagacg gcgtcgcggt
     7021 agagccggtc ggttccgtcg cagagcgctt cgatatccgc tgtcgcgcgg gcgagttcct
     7081 cttccgggtc gccacctgcc gcgatggcgg cacgggcacg ctccagccgc cccatgacgg
     7141 cccgcttgag gtcctcgatc atcgatgcca ttgctaccct accggacgaa cccggcccgc
     7201 ccggcgtaac gggcagcctc tcctgccgcc tcttcgatcc ggaggagctg gttgtacttc
     7261 tcgaccctct caccccgggc cggcgccccg gtcttgaggt ggccggtccg catggcgacg
     7321 gtgaaatcgg cgatgaacgt atcgaccgtc tcaccgctcc ggtgggagac catcgcgccc
     7381 cagccgttct gttgcgccag tctgaccgcg gcgatcgtct cggtcaccgt cccgatctgg
     7441 ttgggcttga tcaggacggc gttcgccacg ccttcccgga tcccccgttc gatccgcgta
     7501 acattcgtca cgaagaggtc gtcgccgacg agttccaccc ggtcgccgat cgcttcggtc
     7561 agcaggcgcc agccgtccca gtcgtcttcg gcaagcccgt cctcgatgac gacgatcggg
     7621 t
//
LOCUS       DAOSEE010000108         7621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_3543_length_7621_cov_197.501850, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000108 DAOSEE010000000
VERSION     DAOSEE010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7621
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3543_length_7621_cov_197.501850"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3206)
                     /locus_tag="PLI76_08360"
     CDS             complement(<1..3206)
                     /locus_tag="PLI76_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOI62085.1"
                     /translation="MGLTERQARAALDHTRSKCVTAGAGTGKTHVLVRKYISLLEEGA
                     SVGSILALTFTEKAAAEMKVRVRQALAEKEGKAWDAVRDEFLWAKVSTFHAFCAAVLR
                     EFPLEANVGPSFTVLDEREAALLRDEAMDDLIYGDPPAEDREAVIGALRAVGAYELKN
                     YLIALSRHRDDAEAFFAALAENEEAVLDAWRAIVEEEREKASLAFRDRAGPSIEVLEG
                     LAARYPGKRDPAEAYLRAVERHLSGTVDALIEIHRGSGFRANMGQRKNWAGDDLNRLR
                     EAYVALKGCIEDNEAALSLALDPDDPFTRATLAFLHDLGKVFHAFIRAVEAEKRRRNA
                     LDFDDLVNRTRRLLVDHEEIAAHFRNRFRFILIDEFQDTDPVQIAIIHVILGREPGTL
                     FIVGDPKQSIYLFREADVAQFRHTRDMIERDFGGETIALDVNFRSAPSVVEFTNAIFG
                     ALMAECGRPWEFLYEPLAPNRSDGAGSVEILLPQKVRDRAAGRRAEADMAAKKIRAIV
                     ERGEKSVWRDGESRPAGYGDIAILLERRTNLAYYEWALARYGIPYRVHAGLGFYERQE
                     LYDIYNILRFLVNDRDDVALYGALRSPYFGFSDARLFAIAGTSLWERLQRSGDPDAIA
                     AATTLRGWLSVARRLPPARLIRRVIDESGITVIFGGTAGGEQAVANVEKLIAIARASS
                     VTLADLVEELGRSIDDGEREGDAPLDLTSSDAVSIMTVHAAKGLEFPVVVVPDLAETP
                     RAGGATIMVEGGLRLGVRIPNPAHDYEREDTPILQVLKAEYRQKEEAERKRLFYVAAT
                     RAKDHLVLCGVRPDEAPETLAAGKTRMAWLAHCLGLSPEAYARGEAVLEALRIPLTLD
                     PASIPVDVGEAAPVCITVPDGIPAVAPAGILPRGTEEHVYSVSEIERYIRDPAASRMP
                     RFSTVPGPVTRGLAVHEVFRGREPAAVLSRYGMDVGRAEEYRDLYERFLASPLMQGRI
                     DDRCEVPFVARVNGVMFEGSIDRLVRRPDGSWVLIDYKTGDAGEYAIQMTVYRHAAEQ
                     ILGRPATPYLYFVDADRWVEVAVDEEQAFAAIERAVRG"
     gene            complement(3193..6231)
                     /locus_tag="PLI76_08365"
     CDS             complement(3193..6231)
                     /locus_tag="PLI76_08365"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOI62086.1"
                     /translation="MSPAVLYRRLPGEGLDAFIAGFRKAAGHDPFGTVFIAPTASLAR
                     EVVRRLGEEGVPLVADAVTTLAGIARKIVSEHAARETLIPAAGSRLIIARLIAGGDYP
                     LLSGAGAVDELAILFSVLTMRKVDYPAALGDLQGAKSAEIAALYRTYTRFLDGHRLID
                     ESTLTARAIRLLSGDFRTVYVYGLFEPMPLERDLLLALRESAEEFFYAIPCAANPAVF
                     ADDGGWLHPDKIIEGKQTSAVAGLFSRGPPGDYRGLIRIAERRDRLDEVRAIAQEIRD
                     LAAAGVRPGDIAVAFPDLDGAAPYVDEVFPDFGVPCTVSVGRPLSASPLVQALLSVLA
                     APARGYRRSDVIALVTSPYLPFTRGSELDILSREARIVAGTWDERLVALAANLDEQPE
                     PEAARDRNAEKIAAIEAARITIRRLFADLATLEGAKTIPDHIAAYRLLLDTWQAPVMP
                     GGGEPDLLDDEARDLAGFTRALAELEELARLLPGDKVPLSEFLSLLGILIAGSRAGRR
                     RNRNTVRVIGIREVPHISVPYLFIAGLVEGDMPRLTTRLPFATDAETRRLGTRSKDDV
                     LREERYHFIAALLAAQSRVYISYPAADDSTPLVRSGFVDAVREAFAPETWGNSDFPDS
                     RLARAGRAGALLARGEAANPPGLTVREAVCRLNIENYHRKGGYDSPYDGLIGDDPAIA
                     GRFGEEAVFSATALETYADCPFRFSMEKVLGLAPLPPADPDLTAQERGNLVHRVACRF
                     YAGWRRDGNGPVTEAGLPDALRRIQATGREEADRFTFTSPAWVAEKEHLLGSPAAGPG
                     LLERFLRHDADMAASGLIPQAFEVSFGLPVSPGEVDLLSVPDAVAVPLSGETLRLRGR
                     VDRVDVLPDGRFAITDYKTGSRHPVLKDIRAGKALQLPLYMRAVEILTGMQGVAGTYY
                     TIRRGDVRNRPVFWDKAMKDCFGCFPGSQQSGVEDIRALVEGSLARAGEYLAGIRGGR
                     FPPRSDAGPCPVYCGFKTICRFDGLRLITAGGEVNADGTD"
     gene            complement(6375..>7621)
                     /locus_tag="PLI76_08370"
     CDS             complement(6375..>7621)
                     /locus_tag="PLI76_08370"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOI62087.1"
                     /translation="QDGNVVAAIETIRDITDRKRAEQEIVRSRRSLADIIDFLPDATF
                     AIDREGIVITWNHEMEKLTGIPAASMIGRGDYEYSLPFYRDRRPMLANLVFIPDAELE
                     SLYTHVQREGNVLVVDTFIPGQEGRPGRFFWAKASPLYDPEGTVTGAIESIRDITERR
                     EMEGRLARSNAELQIAADIQMSFMPKVLPRIAGFDIAARAIMAKEVGGDFFDVIPFEI
                     VALDRGTLGLLIADVSGKGVPAALFMALSRIVVRVNALWHPDPAQAIYDANNIIAGDA
                     KSGMFVTLFFGRLREKDRTLTYVNAGHNPPVVFRSKTGVLEELPATGIILGAFENQDY
                     FSRSLVIEPDDVVVMYTDGVTEAIDARDEPFGVPRLNAIIRENARLPAQGILDRILSD
                     LHEFTGDTPQFDDITLLVIKGT"
BASE COUNT         1251 a         2484 c         2625 g         1261 t
ORIGIN      
        1 atccccctga ccgcccgctc tatggcggca aaggcctgct cctcgtcgac cgcgacctcc
       61 acccagcggt cggcgtcgac gaagtagagg tagggtgtcg ccggccggcc gaggatctgc
      121 tccgcggcat gccggtagac cgtcatctgg atcgcgtact ccccggcatc tcccgtcttg
      181 tagtcgatga gcacccagga cccgtccggc cgccgcacca gacggtcgat ggacccctcg
      241 aacataacac cgttcacccg ggcaacgaac ggcacctcgc agcgatcgtc tatacgcccc
      301 tgcatcaggg gtgaggccag gaaccgctcg tagaggtcgc ggtactcctc cgcccggccc
      361 acatccatgc cgtagcgcga tagcaccgcc gcaggctccc ggccgcgaaa gacctcgtgg
      421 acggcgagac cacgggttac cgggcccgga acggtcgaga accgcggcat acgggacgct
      481 gccggatcgc ggatgtagcg ctcaatctcg cttaccgagt agacgtgctc ctctgtcccg
      541 cgagggagga tacccgccgg agcgacggcc gggataccgt ccgggaccgt tatgcagacc
      601 ggggcggcct cccccacatc gaccggtatc gacgccggat cgagggtgag cgggatacgg
      661 agcgcctcaa ggacggcttc gccccgggca taggcctccg gggagagccc gaggcagtgg
      721 gcgagccagg ccatccgggt ctttccggcc gccagggtct ccggggcctc atccggcctc
      781 actccgcaga ggaccaggtg gtcttttgcc cgcgttgccg cgacgtagaa gagccgtttc
      841 ctctccgcct cctctttctg ccggtattct gccttgagga cctggaggat gggcgtatcc
      901 tcgcgctcgt agtcgtgtgc cgggtttggt atcctgacac cgagcctgag cccgccctcg
      961 accatgatgg tcgccccgcc ggcccgcggc gtctccgcaa ggtccgggac gacgacgacg
     1021 gggaactcta gcccctttgc ggcgtggacc gtcatgatcg agaccgcgtc cgaggaggtg
     1081 aggtcaaggg gcgcatctcc ctcgcgctcg ccgtcatcga tagagcgtcc gagttcttcg
     1141 accagatcgg cgagcgtgac ggaggatgcc cgcgcgatcg cgatcagttt ctcgacgttt
     1201 gcgaccgcct gctcgccccc ggccgtcccg ccgaagatga ccgtgatacc cgactcatcg
     1261 atgacccgcc ggatgaggcg ggcgggcggg agccttcggg cgacggagag ccagccccgg
     1321 agggtcgtcg ccgccgctat ggcgtccggg tcgccggatc gctgcagccg ctcccagagg
     1381 gaggtccccg cgatcgcaaa gagccgggca tcggagaacc cgaagtaagg cgaccgcaac
     1441 gcgccgtaga gcgccacgtc gtcacggtcg tttacaagga accggaggat gttgtagatg
     1501 tcgtatagct cctggcgctc gtagaacccg aggcccgcgt ggacgcggta ggggatgccg
     1561 taccgcgcaa gcgcccactc gtaataggcg aggttcgtcc ggcgttcgag gaggatggcg
     1621 atatcgccgt agccggccgg ccggctctcc ccatcccgcc agaccgactt ctccccccgc
     1681 tcgacgatgg cccggatctt ctttgccgcc atatccgcct cggcccggcg gcccgctgcc
     1741 cggtccctga ccttctgggg gaggaggatc tcgaccgaac ccgcgccgtc agaccggttc
     1801 ggcgccagcg gttcgtagag gaactcccag ggccggccgc actccgccat cagggcgccg
     1861 aatatggcgt tcgtgaactc gacaactgag ggtgcgctcc ggaagttgac gtcgagcgcg
     1921 atcgtctccc cgccgaagtc ccgttcgatc atgtcgcggg tatgccggaa ctgggcgacg
     1981 tcggcctccc ggaagaggta gatggactgt ttcgggtcgc cgacgatgaa gagggttccc
     2041 ggttcccggc cgaggatgac gtggatgatg gcgatctgga cggggtcggt gtcctggaac
     2101 tcgtcgatca ggatgaaccg gaaccggttc cggaaatgcg ccgcgatctc ttcgtggtcg
     2161 acgaggagcc ggcgggtgcg gttgaccagg tcatcgaagt cgagcgcgtt acgccgccgc
     2221 ttctcggcct ccaccgcccg aatgaaggca tgaaagacct tacccaggtc gtggaggaac
     2281 gcaagcgtcg cccgggtgaa ggggtcgtcc gggtcgaggg cgagcgagag ggccgcctcg
     2341 ttatcctcga tgcagccttt gagggcgacg taagcctccc ggaggcgatt gaggtcgtct
     2401 cccgcccagt tcttcctctg ccccatgttt gcccggaacc ccgagcccct gtggatctcg
     2461 atgagcgcat ccaccgtccc ggagaggtgc ctctcgacgg cccggaggta ggcctcggcc
     2521 gggtcccgct tgccggggta gcgggcggca agaccctcga gcacctcgat cgaggggccg
     2581 gcacggtctc ggaacgcgag agaagccttc tcccgctctt cctccacgat cgcccgccag
     2641 gcgtcgagca ccgcctcctc gttctctgca agggcggcaa agaacgcttc ggcgtcatcg
     2701 cggtgcctgg agagtgcgat aaggtagttc ttcaactcgt aggcccctac cgcccggagg
     2761 gcaccgatca ccgcctcccg gtcctccgcc ggcgggtcgc cgtagatgag gtcgtccatc
     2821 gcttcatcgc ggagcagggc cgcctcccgc tcatcgagga cggtaaaaga ggggccgacg
     2881 ttcgcctcga gcggaaactc gcggaggacc gcggcgcaga aggcgtggaa ggtcgagact
     2941 ttcgcccaga ggaactcatc gcggaccgca tcccacgcct tgccctcctt ctcggcgagg
     3001 gcctgccgga ccctgacctt catctccgcg gccgccttct cggtgaaggt cagggcgagg
     3061 atgctcccga cactggcgcc ctcctcaagg aggctgatgt atttccggac caggacatgc
     3121 gtctttcctg tcccggcccc ggcggtgacg cacttgctcc tcgtgtggtc gagcgcggcc
     3181 cgcgcctggc gttcagtcag tcccatcggc gttcacctcc ccgcccgcgg ttatcaaccg
     3241 caagccgtcg aaccggcaga tggtcttaaa tccgcagtag accgggcagg ggccggcatc
     3301 cgaccggggc gggaaccgcc cgccgcgtat accggcgagg tactctccgg cccgggcgag
     3361 ggagccctcg accagcgccc gtatatcctc gacaccgctc tgctgcgatc ccgggaagca
     3421 gccaaaacag tctttcatcg ccttatccca gaagacgggc cggttccgga catccccgcg
     3481 cctgatggta tagtaggtgc cggcgactcc ctgcatcccg gtcaggatct cgaccgcccg
     3541 catgtagagc gggagctgga gcgccttccc cgcccgaata tccttgagaa cagggtgcct
     3601 ggatccggtc ttgtagtcgg tgatcgcgaa ccggccgtcg ggcaggacgt cgacccgatc
     3661 aaccctcccc cgcaggcgga gggtctcgcc gctgagcggg accgcgaccg catcggggac
     3721 cgataggagg tcgacctctc ccggcgagac cgggaggccg aacgagacct cgaaggcctg
     3781 cgggatgaga ccggaagccg ccatatccgc atcgtgccgg aggaaccgct caagcagccc
     3841 cgggccggcc gcgggcgacc cgaggaggtg ctccttctcc gcgacccagg ccgggctcgt
     3901 gaacgtgaac cggtcggcct cttcccggcc ggtggcctgg atccgccgga gggcgtccgg
     3961 gaggccggct tcggtgaccg ggccgtttcc gtcccgccgc cagccggcgt agaaccggca
     4021 ggcgacccgg tggaccaggt tcccccgctc ctgtgcggtc aggtcgggat cggccggggg
     4081 cagcggcgca agaccgagga ccttctccat ggagaaccgg aacgggcagt cggcgtaggt
     4141 ctcgagcgcc gttgccgaga agacggcctc ttcgccgaat cggccggcga tcgccgggtc
     4201 atccccgatc aggccgtcgt agggggagtc gtagccgcct ttccggtggt agttctcgat
     4261 attgagccgg caaacggctt cgcgtaccgt aagccccggg ggatttgccg cctctccccg
     4321 ggcgagaagg gcgccggccc gcccggctct cgcgagccgc gagtccggaa agtcgctatt
     4381 cccccacgtc tccggggcaa aggcttcccg gacggcgtct acgaacccgg agcggacaag
     4441 gggcgtgctg tcgtcggccg cggggtaact gatgtagacc cgggattggg cggcaagaag
     4501 cgccgcgatg aagtggtagc gttcctcgcg gaggacgtcg tctttcgaac gggtgccgag
     4561 gcgccgggtc tcggcatcgg ttgcgaacgg gaggcgggtg gtcagccgcg gcatatctcc
     4621 ctcgacgagg ccggcgatga agaggtaggg gacggagata tgcgggacct cgcggatacc
     4681 gatgacccgg acggtgttcc tgttccgtct ccggccggcc cgggatccgg caatgaggat
     4741 gccaaggagg gataagaact cggaaagcgg caccttgtcg cccgggagga gccgggcgag
     4801 ttcttcgagt tccgcgaggg ctctcgtgaa cccggcaagg tcgcgggcct cgtcgtctaa
     4861 taggtcgggt tctcccccgc cgggcatcac cggggcctgc cacgtatcga ggagcaggcg
     4921 gtaggcggcg atgtgatccg ggatcgtctt tgccccctca agggttgcaa ggtcggcaaa
     4981 gagccggcgg atagtgatcc gtgccgcctc gatcgcggcg atcttttctg cgttccgatc
     5041 tcttgcggcc tccggttccg gctgctcatc gaggttcgcg gcgagtgcta caagccgctc
     5101 atcccaggtg ccggcaacga tccgggcctc ccgcgagagg atgtcgagtt cggacccccg
     5161 ggtaaacggc aggtaggggg aggtcacgag ggcgatgacg tcagaacgcc tgtagccgcg
     5221 ggccggggcg gcgaggaccg agaggagtgc ctggacgagc ggcgatgcgg agagcggccg
     5281 gcccacggag acggtgcagg gaaccccaaa gtcggggaag acctcgtcga cgtagggcgc
     5341 cgccccgtcg agatcgggga acgcgacggc gatgtcgccc ggccggacgc cggcggcggc
     5401 aagatcgcgg atctcctgcg ctatcgcccg gacttcgtcg agccggtccc gccgttctgc
     5461 gatgcggatg agccctcgat agtccccggg cgggccgcgg gagaagaggc cggcgaccgc
     5521 tgaggtctgt ttgccctcga tgatcttgtc ggggtgcagc cacccgccgt cgtccgcgaa
     5581 gaccgccggg tttgcggcgc agggtatcgc gtagaaaaac tcctccgcgg actcccggag
     5641 agcgaggagg aggtcgcgct cgagcggcat cggctcgaag agcccgtaga cgtagaccgt
     5701 ccggaagtcg cccgaaagga gccggattgc ccgggcggta agggtgctct cgtcgatgag
     5761 gcggtgcccg tcgaggaacc gggtgtaggt gcggtagagg gcggcgatct cggcgctctt
     5821 tgcgccctgc agatccccga gggcggccgg gtagtcgacc ttccgcatgg tgaggaccga
     5881 gaagaggata gcgagttcgt caacggctcc ggcaccggag aggagcgggt aatcgccgcc
     5941 ggcgatcagc cgggcgatga tgagccggga cccggccgcg ggaatcaagg tctctctcgc
     6001 cgcgtgttcg gaaacgatct ttctggcgat gccggcgagg gtcgtgaccg cgtcggcgac
     6061 gagcgggacc ccttcctccc cgaggcggcg gacgacctcc cgggcgaggc ttgcggtcgg
     6121 ggcgatgaag acggtcccga acgggtcatg gccggcggct ttccggaaac cggcgatgaa
     6181 cgcatcgaga ccctctccgg gcaatcggcg gtagagaact gctggggaca atcgataatc
     6241 tctccttacg agaggtaggg ggcgggggag ggatgaggat tgttatttgg gtttggggag
     6301 gccctctttt cagtctgcga ccggaggatc ggccgctgcc cccatcgcct cgagcccggg
     6361 gaggaatccc gccatcacgt tcctttgatc accagcagcg tgatgtcgtc aaactggggc
     6421 gtatccccgg tgaactcatg gagatcggat aatatccggt cgaggatccc ctgtgccgga
     6481 agccgggcat tctcccgtat gatcgcgttg agccggggca ccccgaacgg ttcatcccgg
     6541 gcgtcgatcg cctccgtgac gccatcggtg tacatgacaa ccacatcgtc cggctcgatc
     6601 acgagcgacc gggagaagta atcctggttc tcgaacgcgc cgaggatgat accggttgcc
     6661 gggagttcct cgagcacgcc ggttttgctc cggaagacca ccggggggtt gtggccggcg
     6721 ttgacgtagg tcagggtccg gtctttctcg cgcagtctcc caaagaagag ggtgacgaac
     6781 atcccggact tcgcgtcccc tgcgatgatg ttgttggcat cgtagatcgc ctgcgccggg
     6841 tcagggtgcc agagcgcgtt cacccggaca acgatacggg acagcgccat gaagagggct
     6901 gccgggacgc ctttccccga gacgtcggcg atgaggaggc cgagggttcc ccggtcgagt
     6961 gcgacgatct caaaagggat cacgtcgaag aagtcgccgc cgacctcttt ggccatgatt
     7021 gcgcgggcgg cgatatcgaa ccctgcaatc cgggggagca ccttcggcat gaaactcatc
     7081 tggatatccg cggcgatctg gagctcggcg ttcgaccggg caagccgccc ctccatctcg
     7141 cggcgttcgg tgatgtcccg gatcgactcg atggcgccgg tgacggtgcc ctccgggtca
     7201 tacagcgggc ttgccttggc ccagaagaac ctgcccggcc ggccttcctg gcccgggatg
     7261 aacgtatcca cgacgagcac gttcccctcc cgctgcacgt gggtgtagag gctctccagt
     7321 tccgcatccg gtataaagac gaggttggcc agcatcggcc tcctgtcccg gtagaacggg
     7381 agggaatact cgtaatcccc tctcccgatc atggatgcgg cggggatgcc ggtcagtttc
     7441 tccatctcat ggttccacgt gatgacgatg ccctcccggt cgatggcaaa cgtggcatcg
     7501 gggagaaaat cgatgatatc cgcgagactc cgccgggacc ggacgatctc ctgttcggcg
     7561 cgttttcggt cggtgatgtc gcggatcgtc tcgatggcgg caacgacgtt gccgtcctgg
     7621 t
//
LOCUS       DAOSEE010000109         7576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_3562_length_7576_cov_238.950140, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000109 DAOSEE010000000
VERSION     DAOSEE010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7576
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3562_length_7576_cov_238.950140"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..124)
                     /locus_tag="PLI76_08375"
     CDS             complement(<1..124)
                     /locus_tag="PLI76_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOI62088.1"
                     /translation="MKLLFELSGEHPDLPVAELECVGTVLDRRAQVAVAECPDPL"
     gene            complement(308..1075)
                     /locus_tag="PLI76_08380"
     CDS             complement(308..1075)
                     /locus_tag="PLI76_08380"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOI62089.1"
                     /translation="MEEITIHEFDFALINEFFTELERQGPGSPEETIRALGFIGNLSN
                     KTKIADLGCGTGFQTMVLAQNTEATITALDLYAGSIDKLNATAGKLGLQNRVKGIVGS
                     MDDLPFQNDEFDLIWSEGAIANIGFEKGLNHWRRFLKKDGYVAVTYESWFTDERPAEI
                     EKWWLDAVPEISTIGQNISIMQKAGYIPVAAFTLPESCWIDNYFIPQKARQEEFLKKH
                     AGNKTVEDMIAFMRREADLYSKYKQYYGYVFYIGKKM"
     gene            1444..2280
                     /locus_tag="PLI76_08385"
     CDS             1444..2280
                     /locus_tag="PLI76_08385"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOI62090.1"
                     /translation="MEHYAFTALDRTQASDLETLRYYCGLFKEFYPDENERYMDATYL
                     RYVQGTTAIEGNTINLREAQDLLEHNISPAEKRMDDVYEILNFVNLKRYLEGYSGGIT
                     ETFIRKIHEILMNEILRSRGDYRNIQVGIEQVDYEPPPAILIPEEMRALPGWYRQNKR
                     HLNPFELAVVLHAKFEMIHPFVDGNGRVGRALMNFVLERSGYPTLCLGLEHRSSYLNA
                     LVRADDGDYAPIIETLYTIHHQQHGRIADEVRQRLSSKNSRIQEENARIIRQFVLLKK
                     EV"
     gene            complement(2354..3505)
                     /gene="dnaJ"
                     /locus_tag="PLI76_08390"
     CDS             complement(2354..3505)
                     /gene="dnaJ"
                     /locus_tag="PLI76_08390"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOI62091.1"
                     /translation="MGPDSYYDTLGVSRDADDKEIKKAYRDLARKYHPDVCKEPGAEE
                     RFKGINEAYSVLSDPEKRAQYDRMGHSAYSNASRGSYSGGGGFSGGFSADFSGFGDIF
                     DTFFGGGGRQSTGPRPGADLLMQMRITLEEAAFGTEKEVGLDHIEPCPACDGSGSETK
                     KFTTCPTCGGSGQMRQMSQSIFGSFVRMSTCTACGGRGKIPESRCKACGGTGHRRARQ
                     TVKVRIPPGVDTGMRLRMEGYGDAGEYGAPAGDLYIEVTVAPHRAFTRRGDDLETSVD
                     ITPAQATLGSEVEVRTIDGRTVVLDIPAGVQHNTGLKVPGEGVRWQTKPGDMLVRVHI
                     VTPERLTEEERELYRRLLELEGSKPSTKAAKKGKGFFQDVVDKMKESVK"
     gene            complement(3511..5325)
                     /gene="dnaK"
                     /locus_tag="PLI76_08395"
     CDS             complement(3511..5325)
                     /gene="dnaK"
                     /locus_tag="PLI76_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOI62092.1"
                     /translation="MVSDKVLGIDLGTTNSCMAIMEGGRATVIANAEGGRTTPSVVAF
                     TKEGERLVGTVAKRQAITNPNRTIQSIKRKMGSSEKVAIGDKTYTPQEISAMILQKMK
                     LDAEEYLGEKIKKAVITVPAYFNDAQRQATKDAGTIAGLEVLRIINEPTASALAYGIE
                     REGEATVLVYDLGGGTFDVSILQLGDGVFEVKSTAGNNRLGGDDFDNLVVDYLIDEFK
                     KKEGADLRKDPVAMQRLRDAAENAKIELSTVQKTTINLPYITTTESGPKFLDIDLSRA
                     KFESLIADLVDSTLGPVRQALADAKLGADDIDHVLLVGGSTRVPLVQETIKKVLKKEP
                     DKGINPDECVALGAAIQAGVLTGETKDVLLLDVTPLSLGIETLGGIATKLIERNTTIP
                     TRKSQIFSTAADSQTSVEIHVMQGERALAKDNFTLGRFQLTGIPPAPRGIPQIEVTFD
                     IDANGIVHVSAKDLGTGNEQSITIKPQDSRPSETEIQRMMNDAKKFEAEDQKKREEID
                     LRNTADTAVFTAERALKEAKDSIEVAEREKIESAIADLKKALDGDDLDAIKQKMDALT
                     EAVYAVTTKMYQQAAQQKAEGAAKKDDTVVDADYEVKE"
     gene            complement(5330..5839)
                     /locus_tag="PLI76_08400"
     CDS             complement(5330..5839)
                     /locus_tag="PLI76_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOI62093.1"
                     /translation="MKEDTSEPNERQQDRSPAADAASPALEELQKAYDDLNSRHLRLA
                     ADFDNYRKRVAREIDALTTYAIEDFAVELLEVVDNFERAEKAEGAGLREGMEQIKKLL
                     MAILERHGIRPIECRNLPFDPERHEAIACIPSAAEEGIVIDEVIRGYCMQDRIIRCAK
                     VVVSKGMEE"
     gene            6141..>7576
                     /gene="purH"
                     /locus_tag="PLI76_08405"
     CDS             6141..>7576
                     /gene="purH"
                     /locus_tag="PLI76_08405"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOI62094.1"
                     /translation="MKWALLSVWDKAGIVDLAKVLTGHGYRILSSGGTGTALAKAGVP
                     YTDVATYTGFPEMMHGRVKTLHPKVHGGILGRRGIDDAVMQEHGIEPIDLVVVNLYPF
                     EAMRDAGLDIDEIVEYIDIGGPAMVRAAAKNHKYVAVVVDPADYRMVADAVRRGGFTD
                     EERLNLAAKAFARTAAYDGAIVNYLTGIGRQFPEILTVQYRGGRALRYGENPHQAAAV
                     YGDAGIAAEQPLQGKQMSYNNYLDVDAAVGLLREFDDCAVVIVKHNNPCGVATGENLL
                     ATYIAAREVDPVSAYGSIVAMNREVAADVARELTGTFVEVVVAPSFTPEALEIMKAKE
                     NMRVLRLPEPVVQDAIRSIDGGALVQRTEPFREDWQVVTEREPTAHEMRAMHLAWKVC
                     RHTKSNAIIFADEKAVIGIGAGQMNRVESAEIAVRKARRPLAGTAVASDAFLPFPDTL
                     EVAAAAGATALVQPGGSIRDTEVIDAANR"
BASE COUNT         1380 a         2307 c         2229 g         1660 t
ORIGIN      
        1 ccagggggtc cgggcactcg gcgaccgcga cctgggcccg ccggtcgagg acggtgccca
       61 cgcactccag ttccgcgacg ggaaggtcgg gatgctcgcc ggagagctcg aagagcagtt
      121 tcatcccgag gatattggcc ccgcgggggg ataaagcggg aggatgaggg gagcgaccgg
      181 ccgtagggca ggagatcgag aagaacgaag ttcttcgagg ggctggatgc tctaggggtt
      241 gagagccttg cccatctggt tggccgggct tgaaatccgg cgaattctcg gattcccgga
      301 aacaacttta catctttttt ccaatataaa atacgtatcc atagtactgt ttgtatttcg
      361 aatacaggtc cgcctcacgc ctcataaatg caatcatatc ctcaacggtt ttattccccg
      421 cgtgtttttt caagaattcc tcctgccttg ctttttgcgg aataaaataa ttgtctatcc
      481 aacaactttc aggcagtgta aatgcggcaa caggaatata acctgctttc tgcatgatgg
      541 aaatattctg ccctattgtg ctgatctcag gaactgcgtc cagccaccac ttctcaattt
      601 cagcggggcg ttcatcggta aaccatgact catatgttac ggcaacataa ccgtcttttt
      661 tgaggaagcg tctccagtga ttcaagcctt tttcaaaacc tatattggca atagccccct
      721 cggaccatat aagatcgaac tcgtcattct gaaacggcaa atcatccatt gaaccgacaa
      781 taccttttac cctgttctgc aaaccaagtt ttccggctgt tgcgttgagt ttatcaatcg
      841 atccggcgta aaggtcgagg gcggtgatgg ttgcttccgt attttgtgcc agaaccattg
      901 tttgaaagcc tgtgccgcag cctaaatcgg caatttttgt tttgttcgaa agattgccga
      961 taaaacctaa tgccctgatg gtttcttcgg ggctgccggg tccctgccgt tcaagctctg
     1021 tgaaaaattc attgatgaga gcaaaatcaa attcatggat tgttatctct tccattgtca
     1081 tgtcccttat cttctaatta tacccttgac tttttctgcc ttaacaattt ttgctatttt
     1141 atgcgtctgc tcctttgccg caaggtagtt ttcagcgtcc tcagcataag cgttctcagc
     1201 cttcgccttt tgataggact ctcaacgctc tgatgataaa tccccgcttt cgatggcggc
     1261 aattacagcg caacccggtt aacccgggaa taaccttttc atctatcgaa accgagatga
     1321 atgaaaccat gccggaattg accgtaaaga caatcaaggg caataagtac ctttatctca
     1381 gggataaggc taaaactctc cgttctccat aatctaaggt taacgacata cctcgactac
     1441 cggttggaac actatgcctt cacggccctc gacagaactc aggcatctga tcttgagact
     1501 cttcgttact actgcgggct attcaaagag ttctatcccg acgaaaacga gcgatacatg
     1561 gatgcaacgt atctcaggta cgtccagggc acgacagcga tcgaggggaa taccatcaac
     1621 ctccgggaag cacaggacct cctggaacat aacatctccc cggccgaaaa gaggatggat
     1681 gatgtttatg agatcctcaa cttcgtcaac ctcaaacgat atctcgaagg atattccggg
     1741 ggcataacgg agacgttcat cagaaagatc cacgaaatac tgatgaatga gatcctccgg
     1801 agccggggag attaccggaa catccaggtc ggcatcgagc aggtggacta cgaacctcct
     1861 cctgcgatcc tcatccctga ggagatgcgt gctctccccg gctggtaccg gcagaataaa
     1921 cggcacctga acccgttcga actcgccgta gtgctccacg caaagtttga gatgatccat
     1981 ccgtttgtcg atggaaatgg ccgggttggg cgagcgctga tgaattttgt cctggagcga
     2041 tccgggtatc caacgctctg tctcgggtta gaacaccggt catcctacct taacgcgctc
     2101 gtgagggcgg atgatgggga ctatgcaccg atcatcgaga cgctctacac cattcaccac
     2161 cagcagcacg ggcgtatcgc agatgaagtc aggcaaagac tctccagcaa aaacagcagg
     2221 atccaggaag agaacgccag aatcatccgg cagttcgttc ttctgaagaa ggaagtttga
     2281 cccccgatcg ggcagcctcc cggggcgatc cgggtttagc gttccggccg gaggtcaggg
     2341 ggaacagatt ccctcacttc accgactctt tcatcttgtc gacgacgtcc tgaaagaagc
     2401 ctttgccctt ctttgccgcc ttcgtggagg gtttgctccc ctccagttcg agcaggcgcc
     2461 ggtagagttc gcgctcctcc tccgtcaggc gctcgggggt gacgatgtgc acccgcacca
     2521 gcatatcgcc gggcttcgtc tgccaccgca cgccctcgcc cggaaccttc aacccggtgt
     2581 tgtgctgcac gccggccggt atatcgagga cgaccgtgcg cccgtcgatc gtccgcacct
     2641 cgacctcgga gccgagcgtc gcctgggcgg gggtgatatc gacgctcgtc tcgaggtcgt
     2701 cgccccgccg ggtgaacgcc cggtgcggcg cgacggtgac ctcgatgtag aggtcccccg
     2761 ccggcgcacc gtattctccg gcgtcgccgt agccctccat ccggaggcgc atacccgtat
     2821 ccacgcccgg cggtatgcgg accttcacgg tctgccgggc ccgccggtgc ccggtgccgc
     2881 cgcatgcctt gcaccgggac tcgggtatct tcccccgccc gccgcaggcg gtgcaggtgc
     2941 tcatccgcac gaagctcccg aagatcgact ggctcatctg ccgcatctgg ccgctgccgc
     3001 cgcaggtcgg gcaggtggtg aacttcttcg tctcgctccc cgaaccgtcg caggcggggc
     3061 agggctcgat atggtcgagg ccgacctctt tttcggtccc gaacgccgcc tcctcgagcg
     3121 tgatgcgcat ctgcatgagg aggtcggctc cggggcgcgg accggtgctc tgccggcccc
     3181 cgccgccgaa gaaggtatcg aatatatccc caaacccgga gaagtccgcg ctgaacccgc
     3241 cggagaaccc tcccccgccg gagtacgagc cccgggatgc gttgctgtag gcggagtgcc
     3301 ccatccggtc gtactgggcg cgtttctcgg ggtcggagag gacgctgtag gcctcgttga
     3361 tacccttgaa cctctcctcg gctcccggct ccttgcagac atcggggtgg tattttcgtg
     3421 ccaggtcccg gtaggctttc ttgatctcct tatcgtcggc gtcgcgcgag acgccgaggg
     3481 tatcatagta gctgtccgga cccatcggcc tcactctttg acttcgtaat cggcatcaac
     3541 gacagtatca tccttctttg cggccccctc cgccttctgc tgggcggcct gctggtacat
     3601 cttcgtcgtg accgcatata ccgcttcggt cagggcatcc atcttctgct tgatggcatc
     3661 gaggtcgtcg ccatcgagcg ccttcttgag gtcggcgatc gcgctctcga tcttctcgcg
     3721 ctccgcgact tcgatcgaat ccttcgcttc cttcagggcc cgctcggcgg tgaagacggc
     3781 ggtgtcggcg gtgtttctaa ggtcgatctc ctcgcgcttc ttctggtcct ccgcctcgaa
     3841 cttctttgcg tcgttcatca tccgctggat ctcggtctca gaaggacggg agtcctgcgg
     3901 cttgatggtg atcgactgct cgttcccggt gccgaggtct tttgccgaga cgtggacgat
     3961 accgtttgcg tcgatatcga acgtgacctc gatctgcggg atcccccgcg gtgcgggcgg
     4021 gatgcccgtg agctggaacc ggcccagggt gaagttgtcc tttgcaagcg cccgctcgcc
     4081 ctgcatcacg tggatctcga ccgacgtctg gctgtcggcg gcggtcgaga agatctggct
     4141 cttccgtgtc gggatggtgg tgttgcgctc gatcagcttc gtcgcgatgc cgccgagcgt
     4201 ctcgatgccg agcgagagcg gtgtcacgtc gaggagcagg acgtctttgg tctcgcccgt
     4261 cagcacgccg gcctggatgg cggccccgag ggcgacacac tcgtcggggt tgatgccttt
     4321 gtcgggctcc ttcttcagaa ccttctttat ggtctcctgc acgagcggca cccgggtcga
     4381 cccgccgacg aggaggacgt ggtcgatatc gtcggcgccc agtttggcgt cggcgagcgc
     4441 ctgccgcacc ggcccgaggg tcgagtcgac gaggtcggcg atgaggctct cgaacttcgc
     4501 gcgggagaga tcgatgtcca ggaacttggg accgctctcg gtcgtggtga tgtaggggag
     4561 gttgatagtg gtcttctgga cggtggagag ctcgatcttc gcgttctcgg ccgcgtcccg
     4621 gagacgctgc atcgcgaccg ggtctttccg gaggtcggcg ccttccttct tcttgaactc
     4681 gtcgatcagg tagtcgacga ccaggttgtc gaagtcgtcg ccgccgaggc ggttgttgcc
     4741 ggcggttgat ttgacctcga aaacgccgtc gccgagctgg aggatggaga cgtcgaacgt
     4801 gccgccgccg aggtcgtaga cgagcaccgt ggcctcgccc tccctctcga tgccgtaggc
     4861 aagcgcgctt gccgtcggct cgttgatgat ccggaggacc tcaaggccgg caatggtccc
     4921 ggcatccttc gtcgcctgcc gctgggcgtc gttgaagtag gcgggcaccg ttatgacggc
     4981 cttcttgatc ttctcgccca ggtactcctc cgcgtccagc ttcatcttct ggaggatcat
     5041 cgcggagatc tcctgcggcg tgtaggtctt atccccgatg gcgaccttct cgctcgaacc
     5101 catcttcctc ttgatcgact ggatggtgcg gttcgggttc gtgatcgcct gccgttttgc
     5161 gacggtgccg accagccgtt caccctcttt ggtgaacgct acgacggatg gggtggtcct
     5221 gccgccttca gcattcgcga tgaccgtcgc ccgcccgcct tccatgatcg ccatgcacga
     5281 gttggtcgtc ccgagatcga taccaagaac tttatctgaa accatctgtt tattcctcca
     5341 ttccttttga tactacgact tttgcacacc tgatgatcct gtcctgcata cagtatccgc
     5401 gaatgacctc atcgatcacg atcccctcct cggccgccga agggatgcag gcgatcgcct
     5461 cgtgccgttc ggggtcgaat ggaaggttcc ggcactcgat gggccggatg ccgtgccgct
     5521 caaggatggc catgaggagt ttcttgatct gctccatccc ctcgcggagg ccggcgccct
     5581 cggctttctc cgcccgctcg aagttatcga cgacctcaag caactccacc gcgaagtcct
     5641 cgatggcgta ggtcgtgagg gcgtcgatct cccgggccac tcgcttccgg tagttgtcga
     5701 agtctgctgc aaggcgaagg tgacggctgt ttaagtcgtc atacgccttt tgcaactcct
     5761 caagcgccgg agatgctgca tccgccgccg ggctgcgatc ctgttgcctc tcgtttggtt
     5821 cggatgtatc ctccttcata cgcctcacct tcttgaaaat gatataccgt actggtttga
     5881 gtatttattg taatgtagtt ctatatagta ttatcgtatg ctgcacccat tcggctcctg
     5941 caacagcccg gattgacgat cgggcactca ttcgccatta ttctcccctc gttatgtcga
     6001 tcggccggaa agaagggcac cccctctccg gccgcaccga tgagcccggg agcggccggt
     6061 cgctcccttc tccgggcccg ggcgcagccg gttttgcggg tagcgccagt tatatctttc
     6121 catgctgctc acgtgtatcc atgaagtggg cactgctgtc tgtctgggac aaggcaggca
     6181 tcgttgatct cgcaaaggtg ctgaccgggc acggctaccg gattttaagc tcgggcggca
     6241 cagggaccgc gctcgcaaag gccggggtcc cgtataccga cgtcgcgacc tacaccgggt
     6301 ttcccgagat gatgcacggg cgggtcaaga ccctccaccc gaaggtgcac gggggcatcc
     6361 tcggccgccg cggcatcgac gacgccgtga tgcaggagca cggcatcgag ccgatcgatc
     6421 tcgtggtggt gaacctctat cccttcgagg ccatgcggga cgcggggctc gacatcgacg
     6481 agatcgtcga gtacatcgat atcggcggtc cggccatggt gcgggcggcg gcaaagaacc
     6541 ataagtacgt cgcggtcgtg gtggaccctg ccgactaccg catggtggcg gatgcggtcc
     6601 ggcggggcgg gtttacggac gaggagcggt tgaaccttgc cgcgaaagcg tttgcccgga
     6661 cggcggccta cgacggtgcg atcgtgaact acctgacagg tatcggccgg cagttccccg
     6721 agatcctcac cgtccagtac cgtggcggaa gagcgctccg ctacggcgag aacccccacc
     6781 aggccgccgc cgtctacggc gacgccggca tcgccgcgga gcagccgctc cagggcaaac
     6841 agatgtccta caacaactac ctcgacgtcg atgccgcggt cgggcttctc cgggagttcg
     6901 acgactgcgc cgtggtcatc gtcaagcaca acaacccctg cggcgtggcg acgggggaga
     6961 acctccttgc cacctacatc gccgcccgcg aggtcgatcc ggtctcggcc tacggttcga
     7021 tcgtcgcgat gaaccgcgag gtggcggctg atgtcgcccg cgaactcacg gggacgttcg
     7081 tcgaggtggt ggtcgccccg tcgtttacgc ccgaggcgct cgagatcatg aaggcaaagg
     7141 agaacatgcg ggtgctccgg ctgcctgagc cggtcgtgca ggatgccatc cggagcatcg
     7201 acggcggggc cctggtccag cggacggagc ctttccgcga ggactggcag gtcgtgaccg
     7261 agcgggagcc caccgcccac gagatgcggg cgatgcatct tgcctggaag gtctgccggc
     7321 acacgaagag caacgccatc atattcgccg acgaaaaagc ggtcatcgga atcggcgccg
     7381 ggcagatgaa ccgggtcgag tcggcggaga tcgcggtcag gaaggcccgg cggcctcttg
     7441 ccggaacggc ggttgcatcg gacgcattcc tccccttccc cgataccctg gaggtcgcgg
     7501 cggcggcggg cgcgacggcc ctcgtccagc cgggcggctc catccgggat actgaggtca
     7561 tcgatgcggc gaaccg
//
LOCUS       DAOSEE010000110         7551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_3582_length_7551_cov_241.145144, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000110 DAOSEE010000000
VERSION     DAOSEE010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7551
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3582_length_7551_cov_241.145144"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            392..1282
                     /locus_tag="PLI76_08410"
                     /pseudo
     CDS             392..1282
                     /locus_tag="PLI76_08410"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
     gene            complement(2006..3109)
                     /locus_tag="PLI76_08415"
     CDS             complement(2006..3109)
                     /locus_tag="PLI76_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-NAD kinase family protein"
                     /protein_id="HOI62095.1"
                     /translation="MRKTVGFLVNPVAGMGGAVGLAGTDGKVSEALLRGAVPRARDRA
                     VQALSLLRDDDITWYTCAAPMGEDALAGAGIDRFTVVYQPSGPTGAADTKAACRAFLD
                     AGLDLIVFCGGDGTARDVFDAVGRSVPVLGIPAGVKMYSAVFAVNPAAAADLIRRAGG
                     VACRDSEVMDVDEEAYRSGRLSARLYGYLRVPYIPERTQGGKQVFEQQDEERAKDDIA
                     AFINEVMLPETLYIIGAGSTTARIMERLGLTPTLLGVDVVRDGRVVARNTDERTLLAL
                     LDRYPRAKIVVSPIGAQGFVLGRGNQQISPAVLRKTGLSNLVVVATPGKLAATPLLYV
                     DSGDPALDRDIGDSLLVVSGYRVAQRKRVLHPE"
     gene            complement(3106..3942)
                     /gene="trpA"
                     /locus_tag="PLI76_08420"
     CDS             complement(3106..3942)
                     /gene="trpA"
                     /locus_tag="PLI76_08420"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOI62096.1"
                     /translation="MSRIEALFARKNPVFVGFTVAGDPDRETSFGVAKTMVDAGVDLL
                     EIAVPYSDPVADGPVIERAHVRALRAGTTPDDVFALVRRVREYAPPLPLVLFTYYNIV
                     YRRGVDRFFAEAAAAGADGILIVDLPTEESGEVAPSALRHGIDRIVLVAPTTSPERRQ
                     KILGGASGFVYLISLEGVTGERDHLPPNLAGLVGAVREKTDLPLAVGFGVSRPEHVAA
                     VAAAGADGVIVGSALVRVIEEHPGDEAGMHEVLRAVIRALKSGFAPDPDPDMPRRERV
                     EG"
     gene            complement(3939..5111)
                     /gene="trpB"
                     /locus_tag="PLI76_08425"
     CDS             complement(3939..5111)
                     /gene="trpB"
                     /locus_tag="PLI76_08425"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOI62097.1"
                     /translation="MRAGRYGVYGGQYVPETLMSALIELEETYARVREDPEFSRRLSW
                     YLHEYAGRETPLTFCGNLSRDLGCRVYLKREDLLHGGAHKLNNTLGQALMAKFMGKRR
                     LIAETGAGQHGVATAIAGAVLDLPVEVYMGEVDTRRQALNVFRMELLGARVVPVASGT
                     RTLKDAINAAMRDWVANSGDTHYLLGSCVGPHPFPRIVRDFQSVIGEEARRQILEREG
                     RLPDIVVACVGGGSNAIGIFYPFVNDDVRLVGVEAGGEGLISGRHGASLSAGSVGVFQ
                     GALSYLLQDGDGQALETHSIAAGLDHPSVGPEHAMLKDAGRVRYEAVTDAEALHAFRY
                     LSRVEGIIPALESAHAVAYALRAADNLDEDDILVVNLSGRGDKDVADVANVHGGAA"
     gene            complement(5108..5680)
                     /locus_tag="PLI76_08430"
     CDS             complement(5108..5680)
                     /locus_tag="PLI76_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOI62098.1"
                     /translation="MKICGITTVGDARAAAAAGADAIGVVLASPSPRSVTPELAREIF
                     AAVPPFVTTVAVTSTDREEDLATILSSRPDAVQVAGDLALPRGAGVRVIRMLAPGDAL
                     RDDCDAVVIDGSCGTGRAFDPEYARRCVAASPVPVILAGGLSPGNVAAAIRAVRPYAV
                     DVASGVEAAPGIKDERLMRAFVKACRTIEP"
     gene            complement(5677..6435)
                     /locus_tag="PLI76_08435"
     CDS             complement(5677..6435)
                     /locus_tag="PLI76_08435"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOI62099.1"
                     /translation="MILDDIVRATRERLESLDTLPEIDPGAPPHRSLEAAIRACRERR
                     AIIAEVKYASPSRGRIHDGCAPETIAREFAAAGAVGLSVLTEPAYFFGSIENLVRVRR
                     AVTVPILRKDFIVDERQLAETRALGADAVLLIARVLGDRLPAFVDGAYALGLEALVEV
                     HDRSEMERALATDARLIGINNRDLETMRIDLSTTFRLAEAARDAGRIVVSESGITWPY
                     DVRSLGRHCDAFLIGSALMSAKDRCKRLEGFVFA"
     gene            complement(6432..7448)
                     /gene="trpD"
                     /locus_tag="PLI76_08440"
     CDS             complement(6432..7448)
                     /gene="trpD"
                     /locus_tag="PLI76_08440"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOI62100.1"
                     /translation="MIREAIALISSGTDLTPAEAGRVMEEIMRGAATPAQVGGFLTAL
                     RMKGESETEIAAFARAMRAAAVPVVLPAPEMRVDTCGTGGDGAGTFNISTAAAFVAAG
                     AGVPVVKHGNRGVSSRCGSADVLEALGVSVAIPPDKVGEVLAVAGIAFLFAPAYHPAM
                     RYARSARQEIGIRTVFNLLGPLTNPAGAGAHLLGVYDPRLTLPVARVLGDLGVERAMV
                     VHGAGLDEIATAGPTTVAELRDGEVETYTLDCMEFGIPRSPVAALRGGGPEENARLLL
                     SVLAGEDGPARDIVALNAGAAIYLGGKAGDIAAGIARAEASIDSGAALDRLRRLVEAT
                     GGPV"
BASE COUNT         1277 a         2589 c         2490 g         1195 t
ORIGIN      
        1 atgccggcga cgaggccgaa gacgaagacc ccgaggaccg aggggtcgaa ggttgccaca
       61 gagagaggtt gggccgcaac cgggaatata cctgcgatcc ttttgccggg ccattgcagg
      121 cggcgccctg gatacccact tcacactcca ggtcggcatg tcaaccgccg ggcatgcaga
      181 gcgttgcatg agggggtgag gatacagggg cgggcgttgc cgcatcgagc acgaagatga
      241 ggtataatgc ggcgctcccc gtgaggcaac atatggcgcg gcatacgggg tgccgtcccg
      301 gcgaagaacg cactgctcgc cccggtgaaa tcccaccgaa agcattgaga gaggtatttc
      361 aaatcagaga acctatattt atagaacaat aatggtcccc tgggaaatag tcttcgccgt
      421 cgtacctggc ctgattctct tcttctatgg aatcgagaac ttctcccgcg aaatcctcgc
      481 cgttgcaaaa ggcagcttcg cggcaatcct cggcagggtg accgagcggc cgatcctggc
      541 cgcgatactc ggcgccgtcg tcactgccct cgttcagtcg agcgcagcga cggtgatcat
      601 caccgtcagc ctggtcaacg ccgggacgct ctcgttcgtc cagagcctcg ggatcatcat
      661 aggcgcaagc gtcggaacga ccatgaccgc ccagctcgtc gccttcaaga tgacggcgtt
      721 cggacaggtg ctgatcccga tcggtttcct cgtcgggatc ttcgggcggc gttaccggtt
      781 cctcggaaaa cctctcttct acttcggcct cgtcttcttc agtctgaacc tcatatcgac
      841 ggcgctcgtg ccgttccaga acgatcccga gatcatcggc ctcatcacgc ggttctcggc
      901 gctcccgctc gccatcctga tcgggtttct cgtgacgacc atggtgcagt cgagcgccgt
      961 cacgaccggc cttgccgtcg tcctcgccca gcaggggctg ctcacccttc cgcaggcgat
     1021 cccgctcata ctcggcgcgc acatcggttc gaccacgatg gccctcatcg cctccgcccg
     1081 gatgaacctt tactcccggc gggcggcggt ggcccatttc accttgaacc tcgggggagt
     1141 gctcctgatc ctcccgttca ttgatccgtt caccgaactc atcatgacga tcggcggaac
     1201 cgaggcgcag atggtggcaa acgcccacct catatccaac ctgatcacgg cgacggtctt
     1261 cctcatcctc atcaggcagc tcgggcaatt cgttacgtgg ctggtgcccg ggaacgagcc
     1321 tgagatcctg atgcgaacgc actacctcga agacggcatc cccgacgata cgcaccacgg
     1381 gtttgagctg atccgaaagg agctcgggta cgcccacggg gtcacgctcg acctcttccg
     1441 cgcggtgatg acctaccttg cgacgtcaga tgatgcggat taccagaggg tcgagaggct
     1501 caaagacctc aacagccacc ttgacgggaa gatagggcag gcgcttcggg agatatcgcg
     1561 gaggcagctt gcggaccagg aagcgaccga ggtggtcttc ctggtccgga tgtcgcacga
     1621 gatcggccgg ctcggctacc tcggggcgga cctcgggaag ttcttccgga aagcatccga
     1681 aaacgggaac ggcgcctttg tcgtcctcgc agagaagaca gagagtgtct accggatcct
     1741 cgacgcaaac atcgtcgggc tcggccgcca cttcccggtg atcgatgacg cgaccgtcgc
     1801 cgaactccgc gcccgcgatg tcgagctcca gctcgccatc aactggcact accgcgagca
     1861 gctcatctgt ctccgcgaga agggcgaccg cggggacagc cgctacctcg atgccctctc
     1921 catcatcgag gcggcgaacg ccaaagtgcg ggatctccgc aagctcgccg agcagtactc
     1981 gcgcgagaag atggatgaac cggtgtcact ccgggtggag gacccgcttc ctctgcgcca
     2041 cccggtagcc ggagacgacg agaagagagt ccccgatatc ccggtcgagc gccggatccc
     2101 cggaatcgac atacaggagg ggtgtcgcgg cgagttttcc gggcgtggcg accacgacca
     2161 ggttcgataa cccggtcttc cggagcaccg cgggactgat ctgctggttt cccctcccga
     2221 gaacgaaccc ctgggcgccg atggggctca cgacgatctt tgcccgcgga tatcggtcga
     2281 ggagggcaag gagggtccgc tcgtcggtgt tccgggcgac gacccgccca tccctgacga
     2341 cgtcgacgcc aagaagcgtc ggggttagac cgagccgctc catgatccgc gccgtcgtgc
     2401 tcccggcgcc gatgatataa agtgtctccg ggagcatgac ctcattgatg aacgccgcga
     2461 tatcgtcctt cgcccgctcc tcgtcctgct gctcaaagac ctgttttccc ccttgagtcc
     2521 gctccggtat atacgggaca cgcaggtacc cataaagccg ggcagaaagc cggcccgagc
     2581 ggtaggcttc ttcgtcgacg tccatcacct cggagtcgcg gcaggcaacc ccccccgccc
     2641 gccggatgag gtcggccgcg gccgccgggt tgactgcaaa gaccgccgag tacatcttca
     2701 caccggcagg gatcccgagg acggggaccg accgccccac ggcgtcaaag acgtcgcggg
     2761 cagtcccgtc cccgccgcag aagacgatga ggtcgagacc ggcatcgaga aacgcccggc
     2821 acgcggcctt cgtatcggca gcacccgtcg ggccagatgg ctggtagacg acggtaaaac
     2881 ggtcgatacc ggcaccggca agggcatcct cgcccatcgg cgcggcacag gtataccacg
     2941 taatatcgtc gtctctgaga agggagaggg cctggaccgc ccggtcgcgg gcgcggggga
     3001 cggcgccgcg caggagcgcc tccgagacct ttccatccgt ccccgcgagc cccacggcgc
     3061 cgcccatacc ggcgacgggg ttgaccagaa acccgacggt ctttctcatc cctctactct
     3121 ttcgcgccgg ggcatatcag gatcggggtc gggagcgaat ccgctcttga gcgccctgat
     3181 tacggcgcgg aggacttcgt gcatcccggc ttcgtcgccg gggtgctcct cgattacgcg
     3241 gacaagcgcg ctccctacga tgacgccgtc cgcaccggcg gcggcgaccg cagccacgtg
     3301 ttccgggcgc gagaccccga acccgacggc gagcgggagg tccgtcttct cccgcaccgc
     3361 accgaccagc ccggcgaggt tcggggggag atggtcgcgc tcgccggtca ccccctcaag
     3421 cgagatcagg tagacgaacc cggaagcgcc cccgaggatc ttttgccggc gctccggcga
     3481 ggtcgtcggg gcgacgagca cgatccggtc gatgccgtgc cggagggcgg agggtgcgac
     3541 ctcgcccgac tcctcggtgg ggaggtcgac gatgaggata ccgtccgcac cggcggcggc
     3601 ggcctccgca aagaaccggt cgaccccccg acggtagacg atgttgtagt aggtgaagag
     3661 gacgaggggg aggggcggcg cgtactccct gacccgccga acgagggcga agacgtcgtc
     3721 cggggtggtg cccgcccgga gggcgcggac atgcgcccgc tcgatcacgg ggccgtccgc
     3781 caccgggtcg gagtagggga cggcgatctc gaggaggtcg acgcccgcat cgaccatcgt
     3841 cttcgccacg ccaaacgacg tttctctatc agggtccccg gcgacggtga acccgacgaa
     3901 gaccgggttc ttccgggcga aaagtgcctc gatccggctc atgccgcgcc tccgtgaacg
     3961 ttcgcgacgt ctgcaacgtc cttgtcgccc cggccggaga ggttgacgac gaggatgtcg
     4021 tcttcatcga ggttgtccgc cgcccggagg gcgtaagcga ccgcgtgggc ggactcgagc
     4081 gccgggatga tcccttcgac gcgggagagg tagcggaagg cgtggagcgc ttcggcgtcg
     4141 gtgaccgcct cgtagcggac ccttcccgca tccttcagca tggcgtgctc cggcccgacg
     4201 gagggatggt cgaggcccgc ggcgatcgag tgcgtctcga gcgcctgacc gtcgccgtcc
     4261 tggaggaggt aggagagcgc tccctggaag accccgaccg aaccggccga gagcgaagcc
     4321 ccgtgacggc cggagataag tccctcgccg cccgcctcca cgccgacgag ccgcacatcg
     4381 tcgttcacga acgggtagaa gatgccgatt gcgttcgaac ccccgccgac gcaggcgacg
     4441 acgatgtcgg ggagccgccc ttcccgctcg aggatctgcc gccgcgcctc ctcgccgatg
     4501 accgactgga agtcccggac gatccggggg aaggggtgcg ggccgacgca ggagccgagc
     4561 aggtagtggg tatccccaga gttcgccacc cagtcgcgca tcgccgcgtt gatggcgtct
     4621 ttaagtgtcc gcgtcccgga ggcgaccggg acgacccggg cgccgaggag ctccatccga
     4681 aagacattga gcgcctggcg ccgggtgtcc acctcgccca tgtagacctc gacgggcaga
     4741 tcgagaactg cgcccgcgat cgccgtcgcg acgccgtgct gcccggcgcc ggtctcggcg
     4801 atgagccggc gcttgcccat gaacttcgcc atgagcgcct ggccgagggt gttgttcagt
     4861 ttgtgggcgc cgccgtggag gagatcctcc cttttcaggt agacacggca gccgaggtcg
     4921 cgggagaggt tcccgcagaa ggtaagcggt gtctcccggc cggcgtactc gtgcaggtac
     4981 caggagagcc ggcgggagaa ctccgggtcc tcgcggaccc gggcataggt ctcctcaagc
     5041 tcgatcaacg cgctcatcag ggtttcgggc acgtactgcc cgccgtatac tccgtatcgt
     5101 cctgctctca tggttctatc gtcctgcatg ccttcacgaa cgccctcatg agacgctcgt
     5161 ccttgatccc cggcgccgcc tcgacgcccg aagcgacgtc gacggcgtaa ggccttaccg
     5221 cccggattgc cgcggcgacg tttccggggg aaagcccccc cgcgaggatg accggcaccg
     5281 gggaggcggc cacgcatctt cgtgcgtatt cggggtcgaa cgcccgcccg gtgccgcagc
     5341 tgccgtcgat gaccaccgcg tcgcagtcgt cccggagagc gtcgccggga gcaagcatcc
     5401 ggatgacccg gacccccgcg ccccggggga gggcgaggtc gcctgccacc tggaccgcgt
     5461 ccggccgcga ggagaggatc gtcgcgaggt cctcctcccg gtcggtcgaa gtgacggcga
     5521 ccgtggtcac gaacggcgga accgccgcga atatttcccg cgccagctcc ggggtcaccg
     5581 accggggcga ggggctcgcg agcaccacgc cgatcgcatc ggctccggcc gcggcggcgg
     5641 cacgggcgtc gccgacggtg gtgatcccgc agatcttcac gcgaatacga atccctccag
     5701 ccgtttgcac cgatctttag ccgacatcag ggcggacccg atcaggaacg catcgcaatg
     5761 cctcccgagg ctccggacat cgtagggcca cgtgatgccg ctctcggaga ccacgatcct
     5821 ccccgcgtca cgtgccgctt ccgcgaggcg gaaggtcgtc gagaggtcga tcctcatcgt
     5881 ctccaggtcg cggttgttga tcccgataag tctcgcatcc gtcgcgagag cgcgttccat
     5941 ctcgctcctg tcgtggacct cgacgagcgc ctcaagaccg agcgcgtatg ccccgtcgac
     6001 gaacgcgggg aggcgatccc cgagcacccg ggcgatcagg aggacggcgt cggcgccgag
     6061 cgcccgggtt tctgcaagct ggcgctcgtc gacgatgaag tctttcctga ggatcggcac
     6121 cgttaccgca cgccgcaccc ggacgaggtt ttcgatgctc ccaaaaaaat aggcaggttc
     6181 ggtcagcacc gagagcccga cggctccggc ggcggcgaac tccctcgcga tcgtctcggg
     6241 ggcgcagccg tcgtggattc tgccgcgcga cggggaggcg tacttcacct cggcgatgat
     6301 cgcgcgtctc tcccggcacg cacgtatggc ggcctcaagg ctccggtggg gcggagcgcc
     6361 gggatcgatc tccggaagag tgtcgaggct ctccaggcgc tcccgggtcg ccctgacgat
     6421 atcgtcgagg atcataccgg gcctccggtc gcctcgacca gccggcggag ccgatcgagc
     6481 gccgcgcccg agtcgatcga agcctcggcg cgggcgatgc cggcggcgat gtcgccggcc
     6541 tttccaccga ggtagatcgc cgccccggcg ttgagggcga cgatgtcccg ggcggggccg
     6601 tcctcccccg cgaggacgga gaggaggagc cgggcgttct cctccggccc gccgccccgg
     6661 agtgcggcca caggcgagcg cgggatcccg aactccatgc agtcgagcgt atacgtttcg
     6721 acctcgccgt cccggagctc cgcgaccgtc gtcggcccgg ccgtcgcgat ctcgtcgaga
     6781 cccgcgccgt ggaccaccat cgcccgctcc accccgagat ccccgagcac ccgcgcgacc
     6841 ggaagggtca ggcggggatc gtagacgccg aggaggtgag ccccggcacc tgccgggttc
     6901 gtgaggggac cgaggaggtt gaagaccgtc cgtatcccga tctcctgccg ggccgatctg
     6961 gcgtaccgca tcgccgggtg gtaagccggg gcgaagagga aggcgatccc ggcgaccgcg
     7021 aggacctccc cgaccttatc gggcgggatc gcgacggaga cgccgagcgc ctcgaggacg
     7081 tcggccgaac cgcaccggct cgatacgccc cggtttccgt gcttcacgac cgggacgccg
     7141 gccccggccg cgacaaaggc ggccgcggtg ctgatgttga acgtccccgc accgtcgccc
     7201 ccggtcccgc aggtatccac ccgcatctcc ggggcgggga gaacaaccgg gacggccgcc
     7261 gcccgcatcg cccgggcgaa cgccgcgatc tccgtctcgc tctccccttt catccgcagt
     7321 gccgtgagga acccgccgac ctgggcggga gtcgccgcgc cccgcatgat ctcctccatc
     7381 acccttcccg cctcggcggg ggtgaggtcc gtgcccgagg agatcagggc aattgcttcg
     7441 cggatcatgc cgcacccccc atgccggaga ggaagttttt catcaggcgg ttcccgtcgg
     7501 gggtgaggat gctctccggg tggaactgca ccccctcgat cgggaactcc c
//
LOCUS       DAOSEE010000111         7318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_3721_length_7318_cov_189.172931, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000111 DAOSEE010000000
VERSION     DAOSEE010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7318
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3721_length_7318_cov_189.172931"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PLI76_08445"
     CDS             <1..305
                     /locus_tag="PLI76_08445"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867931.1"
                     /note="catalyzes the reversible formation of fructose
                     1,6-bisphosphate from glycerone phosphate and
                     D-glyceraldehyde 3-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HOI62101.1"
                     /translation="ARVAAELGADIVKTVYTGDADSFREVTRGCPVPVVVAGGSKTDD
                     RAILDLVEGAMEGGAAGISIGRNAFQHAAPDRLIRAAALIVHEGRSAEEAMEILEV"
     gene            311..1096
                     /locus_tag="PLI76_08450"
     CDS             311..1096
                     /locus_tag="PLI76_08450"
                     /EC_number="2.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate
                     synthase"
                     /protein_id="HOI62102.1"
                     /translation="MIGKEIRLERIMDRNTGRAVIIPMDHGFTMGQIEGLRNMTGIVS
                     EVSEAGANAIVLHKGIVKGGHRKRGKDIGLIVHLSASTSMNPDPNDKVLVCTVEEAVA
                     LGADAVSIHINLGAPNESRMIESAGAVVRDCNRWGIPLLIMIYPRGKGIDSTSPQAIG
                     HCVRVAEELGADLIKTNYTGNPETFRPITAACSVPVMIAGGEKGGDLETLTTIRDAIG
                     AGAAGVCMGRNAFQRDDPGRFIGAICRVVHEGISPADALERGR"
     gene            1093..2079
                     /locus_tag="PLI76_08455"
     CDS             1093..2079
                     /locus_tag="PLI76_08455"
                     /EC_number="1.4.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase II"
                     /protein_id="HOI62103.1"
                     /translation="MKQFWVDIRPWRKDLATTAIESGANVLVVDDADRVRELGRVMTA
                     AENGDLVPGKDVFEVEIVDKAAEEEALRLSREGYVIVRTGDWTVIPLENLVAQSDRIV
                     AAVTTADEAAVALTVLERGTAGVLLTTDDPAEIRRVAGVVAGSGAEVPLTVFEVTRIA
                     PVGMGDRVCVDTCSLLVDGEGMLVGNTSSAFLMVHPETLENPYVAPRPFRVNAGAVHA
                     YILLPGGKTAYLADLAAGDKVLVAEHTGPTHDAVVGRVKIERRPLLLVEAKAGSANVS
                     LVLQNAETIRLVREGGTAVSVANLAVGDKVLGSLMEGGRHFGIAVKETIREK"
     gene            2085..2909
                     /locus_tag="PLI76_08460"
     CDS             2085..2909
                     /locus_tag="PLI76_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOI62104.1"
                     /translation="MLAGIIGGTGQMGRFFAEVFRAAGWDVIVSGRTTPLTGRDIAGE
                     ADLVMVSVPIRATVAVIREVAPLLSEEQIFCDLTSLKAEPVGAMLASRAEVIGLHPMF
                     GPGAVSLRGQTIVATPARCRPATLEGLLSIFRDQGAAITLSTPEDHDRMMAVIQGLTH
                     FGTLAKAETIRRTGADVGETLAFTSPVYRIEMGFVGRLLAQDAGLYGDILQMNPAVPE
                     VLAAFEDAVRTLRRIVESGDGEEFRMLFAENAGHYASYLHTATEETDDLINHVVNR"
     gene            2906..3700
                     /locus_tag="PLI76_08465"
     CDS             2906..3700
                     /locus_tag="PLI76_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HOI62105.1"
                     /translation="MTVLTLGPAGTFSHDLAVRLYGDDVLLLPTIRAIIGRVAAGAVK
                     GLVPIENSEAGGVGETLQGFLEFDVAITGEAYMPVRHHLAARGEPERLSVIYAHPQTH
                     EQCSVLLDSLGVEVVHTSSNAASAVAMQGDERAGAVVPEMAAVLYGLPIALRDIQNSR
                     ENTTRFVEISPAPGREVGCTKCSLLVDPKADRVGLLADLLGIFARRGINLTRIESRPS
                     RRGIGSYLFFIDLEISEGWREAKEELKRMTSVRELGCYARLEVPGV"
     gene            3697..4965
                     /gene="aroA"
                     /locus_tag="PLI76_08470"
     CDS             3697..4965
                     /gene="aroA"
                     /locus_tag="PLI76_08470"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOI62106.1"
                     /translation="MIVRVSQAGPVDAAFEAPPSKSYTHRALIAGALATGRSRIAGPL
                     RAADTELTARGLEQLGVPIEWLPGEVTVTGCNGAFPAPGEVTIDCGNSGTTLRLLTSA
                     ALLARQAVVLTGSARMLERPVGPLAGALQALGGDVAFTGRPGFPPIRVSGSLRGGKAA
                     IDGSVSSQFVSSVLMAAPYADEDVELTLPATVASRSYLDVTADVMLRFGARLDRRGYE
                     WFRVESGAPYRGRDYQIEGDYSSASYFFAIAAVCGGRVTVRGLNPASVQGDRRFLDAL
                     EAMGCRVAAAGDAVTVERAGDLRGIDIDMSSSPDTVQTLAAVAAVAASPTTITGIAHL
                     QYKESDRVGVTADALRRMGAGASVTDDSLTITPAPLHGVLVDPHDDHRTAMAFTVLGL
                     AVGGMAIRDPGCVEKSFPGFWEALYGQGLL"
     gene            4962..6314
                     /gene="aroE"
                     /locus_tag="PLI76_08475"
     CDS             4962..6314
                     /gene="aroE"
                     /locus_tag="PLI76_08475"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOI62107.1"
                     /translation="MKIVLVGFRGTGKTSVGRLLAERLALPFFDTDTLIERRAGVSIP
                     DIFGRHGEAHFRALEREVVASLRDAEGIISTGGGVVLDPANVADLRWGGRVFLLSAPP
                     DVIQERIAGSDRPGLTNLPPAEEVQALLAARKEAYIGAADVCIDTGRRTPGEVADMVL
                     REAGISGDEKSERNTLLERFALADIGDMVDRDPGLMVCAIAGNPCAHSKSPLLYNRLF
                     ARFGMRYHYTRFEWPDAGAIVRLASLLPTKGLSVTIPFKADVMRHLDLVDSHAAAIGA
                     VNTVIRCGGRMYGYNTDWLGVRAPLAHRRGGRAVVLGAGGAAAAAVYALLSLDMDVTV
                     LARTPDAARRLAERFTCRWGGLDEFRGSDADVVVHATPVGMEPDTRSLLSASDFWAGT
                     TVFDLVYTPPETPLIRAAKEAGCETIPGTEMFVHQAVAQFRLMTGIAVTPELVREMLR
                     "
     gene            6311..7312
                     /locus_tag="PLI76_08480"
     CDS             6311..7312
                     /locus_tag="PLI76_08480"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOI62108.1"
                     /translation="MNTFGRTFRCTTFGESHGLAVGVVIDGCPPGIALAEADIQPYLD
                     RRRPGKGPLESARREPDRVEILSGIFEGRTTGAPLALVVRNRDVRSEDYDALREVFRP
                     GHADYTWQAKYDLRDHRGGGRSSGRETLARVAAGAVAVRCLAPHGISIRGRVVEVHAA
                     TEPETMEAEIRAARDAGDSVGGIVEVTAAGCPAGLGDPVFGKLDAAIAGAMMGIGAVK
                     GVEIGEGFAAARLFGSEMNDPIERTGFSSNHAGGTLGGISTGQDIVVRLAVKPTPSIR
                     RVQRTVDISGTEREISVGGRHDPCIAPRIVPVAECMMALVILDAMLEQAKYRGFGGE"
BASE COUNT         1180 a         2338 c         2668 g         1132 t
ORIGIN      
        1 ccgcacgggt ggcggcggag ctcggcgccg atatcgtcaa gaccgtctac accggcgatg
       61 cggactcatt ccgcgaggtg acccggggat gcccggtgcc ggtcgttgtc gcgggaggct
      121 cgaagaccga cgaccgcgcg atcctcgacc tggtggaggg tgcgatggag ggcggtgccg
      181 ccggtatatc gatcgggagg aacgccttcc agcacgcggc gcccgaccgt ctcatccgcg
      241 ccgcggcgct gatcgtccat gaggggcggt cggcagaaga agcaatggag attcttgagg
      301 tgtgatgaat atgattggaa aagagatccg cctggagcgg ataatggaca ggaacaccgg
      361 tcgcgccgtg atcatcccca tggatcacgg gttcaccatg ggccagatcg aggggctccg
      421 caacatgacc gggatcgtca gcgaggtgag cgaggccggg gcgaacgcca tcgtcctcca
      481 caaaggcata gtgaagggag ggcaccggaa gcgcgggaag gacatcggcc tcatcgtgca
      541 cctctccgcg agcacgtcga tgaacccgga cccaaacgac aaggtactcg tctgcaccgt
      601 ggaggaggcg gtcgcgctcg gcgccgacgc ggtctcgatc cacatcaacc tcggcgcgcc
      661 gaacgaatca cgcatgatcg agtcggcggg cgcggtggtg cgggactgca accgctgggg
      721 gataccgctc ctgatcatga tctacccccg cggcaagggg atcgattcga catcgccgca
      781 ggcgatcggg cactgcgtcc gggttgccga agaactcggg gccgacctta tcaagacgaa
      841 ctacaccggg aacccggaga cgttccgccc gatcaccgcc gcctgctcgg tgccggtgat
      901 gatcgccggc ggcgagaaag ggggagacct tgagacgctc acgaccatcc gcgacgccat
      961 cggggcgggc gcggccggtg tctgcatggg acggaacgcc ttccagcgcg acgaccccgg
     1021 ccggttcata ggcgcgatct gccgggtggt gcacgagggc ataagcccgg ccgacgcgct
     1081 ggagcgtggg cgatgaaaca gttctgggtc gatatcaggc cgtggaggaa ggatcttgcg
     1141 accacggcga tcgagagcgg tgcgaatgtc ctggtcgtcg acgacgccga ccgtgtccgg
     1201 gaactcggac gggtgatgac cgccgcggaa aacggcgacc tcgtccccgg aaaagatgta
     1261 tttgaggttg agatcgtcga taaagcggcc gaggaggagg ccctccggct ctcccgcgag
     1321 ggttacgtca tcgtccggac gggggactgg acggtcatac cgcttgaaaa cctcgtcgca
     1381 cagtcggacc ggatcgtcgc cgcggtcacg accgccgacg aggcggcggt cgccctgacc
     1441 gttctcgagc gcgggacggc gggcgtcctc cttacgaccg atgatccggc ggagatccgg
     1501 cgcgttgccg gagtggtcgc aggttcgggc gcagaggtcc ctctcaccgt attcgaggta
     1561 acccggatcg cccccgtcgg catgggggac cgggtctgcg tggatacctg ctcgctcctc
     1621 gtcgacggag aagggatgct cgtcggcaac acgtcatcgg cgttcctgat ggtgcacccc
     1681 gagaccctgg agaaccccta cgtggcgccg cgcccgttcc gggtgaacgc cggggcggtg
     1741 cacgcctaca tcctcctccc cggcgggaag accgcatacc tcgccgacct tgcggcgggg
     1801 gataaggtgc tcgtcgcgga gcataccggc ccgacccatg acgcggtcgt cggcagggtg
     1861 aagatcgagc gccgcccgct cctcctcgtc gaggcgaagg ccggcagcgc aaacgtgagc
     1921 ctggtcctcc agaacgccga gacgatacgg ctcgttcgcg agggcggcac ggccgtatcg
     1981 gtcgcaaacc tcgcggtcgg cgacaaagtg ctcggcagcc tcatggaggg cgggcggcac
     2041 ttcggcatcg ccgtgaagga gacgatccgg gagaagtgac ggcgatgctt gcagggatca
     2101 tcggcggcac cgggcagatg gggcgcttct ttgcggaggt cttccgggcg gccggctggg
     2161 acgtgatcgt ctcggggaga acaacgccgc tcaccggccg ggatatcgcg ggggaggcgg
     2221 atctcgtcat ggtatcggtg cccatccgcg cgaccgtcgc ggtgatccgg gaggttgccc
     2281 cgctgctctc ggaggagcag atcttctgcg acctcacctc gctcaaggcc gaaccggtcg
     2341 gggcgatgct cgcatcccgc gcggaggtga tcgggctcca ccccatgttc gggccgggtg
     2401 ccgtatcgct ccgggggcag acgatcgtcg ctacgccggc ccgatgccgc cccgcgaccc
     2461 ttgaagggct cctctccatc ttccgcgacc agggagcggc gatcaccctc tcgacgccgg
     2521 aagaccacga ccggatgatg gcggtgatcc aggggctgac tcacttcgga acgcttgcaa
     2581 aagcagagac cattcgccgg accggcgccg atgtcgggga gaccctcgcg ttcacgagcc
     2641 ccgtctaccg gatagagatg gggtttgtcg gccggctcct cgcccaggac gccggactct
     2701 acggcgatat cctgcagatg aatccggcag tcccggaggt gcttgcagcg tttgaggatg
     2761 cggtccggac gctccgcagg atcgtggagt ccggcgacgg tgaggaattc cggatgcttt
     2821 ttgcggagaa cgccggccat tacgcctcct acctccatac cgcgacagaa gagacggacg
     2881 acctcatcaa ccacgtggtg aaccgatgac ggtcctgacc ctcgggccgg ccgggacgtt
     2941 cagccacgat ctcgcggtcc ggctctacgg cgacgacgtc ctcctcctgc cgaccatccg
     3001 cgccatcatc gggcgcgtgg cggccggcgc agtaaagggc ctcgtcccga tcgagaactc
     3061 ggaggccggc ggcgtggggg agaccctcca ggggtttctg gagttcgacg tcgccatcac
     3121 cggggaggcg tacatgcccg tccggcacca cctcgcggcc aggggggaac cggagcggct
     3181 ctcggtcata tacgctcacc cgcagaccca cgagcagtgc tccgtcctcc tcgacagcct
     3241 cggcgtggag gtcgtgcaca cgagcagcaa cgccgcaagc gccgtcgcca tgcagggcga
     3301 tgaacgcgcc ggcgcggtcg tccccgagat ggcggcggtg ctctacggcc tcccgatagc
     3361 cctccgggat atacagaaca gccgggagaa caccacccgg ttcgtggaga tatcccccgc
     3421 tcctgggagg gaggtggggt gcacgaagtg cagcctcctc gtggacccga aggccgaccg
     3481 ggtcggtctc cttgccgatc tgctcggcat cttcgcccgg cgcggtatca acctgacccg
     3541 gatcgagtcg cgcccttccc ggcgcggtat cgggagctat cttttcttca tcgacctcga
     3601 gatatcagaa ggatggcggg aggcaaagga ggaactcaaa aggatgacca gcgtcaggga
     3661 gctcgggtgt tacgcccgac tggaggtgcc gggagtatga tcgtcagggt atcgcaggcc
     3721 ggcccggtcg atgcggcgtt cgaggccccg ccctccaaga gttacactca ccgggctctg
     3781 attgcaggag cgctcgccac cgggaggtcg cggatcgcag ggccgctcag ggcggccgat
     3841 acggaactga ccgcccgcgg gctggaacaa ctgggggtgc cgatcgagtg gctgccggga
     3901 gaggtcaccg tcacggggtg caacggggcc ttcccggccc cgggtgaggt cacgatcgac
     3961 tgtgggaact cggggacgac gctgcgtctc ctcacctcgg cggcgcttct tgcccggcag
     4021 gcggtggtgc tgacggggag cgcccggatg ctcgaacgcc cggtggggcc gcttgccggt
     4081 gcgctccagg cactcggcgg cgacgtcgcg ttcaccgggc ggccggggtt cccgcccatc
     4141 cgggtctccg gcagcctccg gggcggaaag gctgcgatcg acgggagcgt gagcagccag
     4201 ttcgtctcgt ccgtcctgat ggcggccccg tatgcggatg aggacgtgga gcttacgctc
     4261 ccggcaacgg tggcctcgcg gtcctacctc gacgtgaccg cggacgtgat gctccggttc
     4321 ggcgcccggc tcgatcggcg gggctacgag tggttccggg tggagagcgg cgcgccctac
     4381 cgggggaggg actaccagat cgagggcgac tactcgtcgg cctcctactt cttcgcgatc
     4441 gcggccgtct gcggcgggag agtcacggtc agggggttaa acccggcgtc ggtccagggc
     4501 gaccggcggt tcctcgatgc cctcgaggcg atgggctgcc gggtggcggc ggccggggat
     4561 gcggtgacgg tggagcgggc gggcgacctc cggggcatcg atatcgatat gtcgtcgtcc
     4621 cccgacaccg tccagacgct cgcggcggtg gcggcagtgg ccgcgtcgcc gacgacgatc
     4681 accgggatcg cgcacctgca gtacaaggag agcgaccgcg tcggggtgac ggcggatgcc
     4741 ctccggagga tgggtgcagg cgcctcggtc acggatgact ctctcacgat cacgcccgct
     4801 cccctccacg gcgtcctcgt cgacccgcac gacgatcacc ggacggccat ggcgtttacc
     4861 gtgctcgggc tcgcggtcgg ggggatggcg atccgcgatc ccgggtgcgt ggagaagtcg
     4921 ttccccgggt tctgggaggc gctctacggg caggggctgc tatgaagatc gtcctcgtcg
     4981 gtttccgggg gaccggaaag acgtcggtcg ggcggctcct cgccgaacgg ctcgcgctgc
     5041 cgttcttcga taccgacacc ctgatcgagc gccgggcggg ggtctcgatc ccggatatat
     5101 tcgggcggca cggtgaggca cacttccggg ccctcgagcg ggaggtggtc gcgtcgctcc
     5161 gggacgcgga gggcatcatc agcaccggcg gcggggtggt tctcgacccg gcaaacgtcg
     5221 cggacctgcg gtggggcggc agggtcttcc tcctctcggc cccgcccgac gtcatccagg
     5281 agcggatcgc cggaagcgac cggccggggc tgacgaacct cccaccggca gaggaggtac
     5341 aggcgctcct cgccgcaagg aaggaggcat acatcggtgc ggccgacgtc tgcatcgata
     5401 ccgggaggcg cacgccgggt gaggtggcgg acatggtcct ccgggaggcc ggcatatccg
     5461 gggacgagaa gagcgagcgg aataccctcc tcgaacggtt cgctcttgcg gatatcgggg
     5521 atatggtcga ccgcgacccg ggtctcatgg tgtgcgccat cgccggcaac ccctgcgccc
     5581 acagcaagag cccgctcctc tacaaccggc tctttgcgcg ctttgggatg cgttaccact
     5641 acactcgctt cgagtggccg gacgcaggcg ccatcgtccg cctcgcctcc ctccttccca
     5701 caaaggggct ctcggtcacg atcccgttca aagccgatgt catgcgccac ctcgacctcg
     5761 tcgacagcca cgcggcggcg atcggggcgg tgaacaccgt catccggtgc ggcggccgga
     5821 tgtacggcta taacaccgac tggttgggcg tgcgggctcc gctcgcccac cggcggggcg
     5881 ggcgggcggt tgtgctcggc gccgggggtg cggcggcggc ggcggtctac gccctcctct
     5941 cgctcgacat ggacgtgacg gtgcttgccc gaacgccgga tgcggcacgg cggctcgcgg
     6001 agcgctttac gtgccggtgg ggcgggcttg atgaattcag ggggagcgac gccgacgtgg
     6061 tggtgcacgc aaccccggtc ggcatggagc ccgacacccg gagcctgctc tctgcgagcg
     6121 acttctgggc ggggacgacc gtctttgacc ttgtctacac gccgccggag acgcccctca
     6181 tccgggcggc gaaggaggcc gggtgcgaga cgataccggg cacggagatg ttcgtccacc
     6241 aggcggtggc gcagttccgg ctgatgaccg gtatcgcggt cacgccggaa ctggtgcggg
     6301 agatgcttcg atgaacacgt tcggaagaac cttcagatgc acgacgttcg gcgagagcca
     6361 cgggttagcg gtgggcgtgg tcatcgacgg ttgcccgccg gggatcgccc tcgcggaggc
     6421 ggacatccag ccctacctcg accggaggcg gccggggaag ggtccgcttg agtcggcgcg
     6481 gcgggagccc gacagggttg agatcctctc ggggatattt gaggggcgga caaccggcgc
     6541 cccactggcg ctcgtcgtcc ggaaccggga tgtccggtcg gaggactacg atgccctccg
     6601 ggaggttttc cggccggggc acgccgacta cacctggcag gcaaagtacg atctccgcga
     6661 ccaccggggc ggcgggcgga gttcgggcag agagaccctt gcccgggtcg cggccggggc
     6721 ggtggcggtg cgctgtcttg cgccgcacgg catatcgatc cgcgggaggg tcgtcgaggt
     6781 gcatgccgcg acggaaccgg aaacgatgga ggcggagatc cgtgccgccc gcgacgcggg
     6841 ggactccgtg ggcggtatcg tcgaggtgac ggcggccggg tgcccggcag gcctcgggga
     6901 tccggtcttc gggaagctcg acgccgccat cgccggggcg atgatgggca tcggtgcggt
     6961 gaagggcgtc gagatcgggg aagggttcgc cgccgcccgg ctcttcggga gcgagatgaa
     7021 cgacccgatc gagagaaccg gattttcatc caaccacgcg ggcgggaccc tcggcgggat
     7081 cagcaccggg caggatattg tcgtccggct cgccgtgaaa ccgacgccct cgatacggcg
     7141 ggtgcaacgg accgtcgata tctccgggac ggagcgggag atctccgtcg gcggaagaca
     7201 cgacccctgt attgcgccgc ggatcgtccc ggtcgcggag tgcatgatgg cgctcgtcat
     7261 actcgatgcg atgcttgagc aggcgaaata ccgggggttt ggaggggagt agggcggt
//
LOCUS       DAOSEE010000112         7144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_3827_length_7144_cov_209.729440, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000112 DAOSEE010000000
VERSION     DAOSEE010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7144
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3827_length_7144_cov_209.729440"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..273)
                     /locus_tag="PLI76_08485"
     CDS             complement(109..273)
                     /locus_tag="PLI76_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62109.1"
                     /translation="MRVFWPGTELVRQRLQAAVERFVEDENILRATLSSSFAGGRAVS
                     GSDLEVLARD"
     gene            complement(601..1101)
                     /locus_tag="PLI76_08490"
     CDS             complement(601..1101)
                     /locus_tag="PLI76_08490"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOI62110.1"
                     /translation="MNAKTFAALAGAMRKDAAVGQKTVSGLLNWNGGAHSTMIVRNFV
                     IPADEPAALGGTDRGASPMELVLAGLMSCVAVSIAYSAAEEGIDLHAIAIDVEGDIDL
                     RGLFGVADDVRPGFEEIRLIVRVDADASEETIEDLVHRGYRRSPAVDSLTGRVPVRVC
                     IGREGV"
     gene            complement(1098..3170)
                     /locus_tag="PLI76_08495"
     CDS             complement(1098..3170)
                     /locus_tag="PLI76_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOI62111.1"
                     /translation="MTPEKPVQGRSPPNRLIHEQSPYLLQHADNPVDWHPWGEEAFAK
                     ARKEDKPVFLSIGYSACHWCHVMEKESFADPVVAKLLNDVFVCIKVDREERPDIDQIY
                     MAAAHVLAGSGGWPLTILMTPDKKPFFAASYIPKESRYGMTGLLDLIPRISKVWRDRR
                     GELEEAGDCVFGALQSAPSPEGDIPDAAIDEAYETFLRIFDEENGGFGDAPRFPAPHN
                     LIFLLRYAHGTGKEPAYAMAEKTLHAMRQGGIFDQIGWGFHRYATDVAWAVPHFEKML
                     YDQALLAMAYTEAYLATGREEFARTARETIAYVLRDMTGPGGGFYSAEDADSEGEEGK
                     FYTWTKAEILDVLGEDGERFSRIFGIADRPGRQVLRRRRPLASWAHEFSMPEEDLAWF
                     VEGARQRLFQAREQRVRPAKDDKILTDTNALMIAALAGAARAFDDPGYLAAAEKALAF
                     VLENLRDEDGRLLHCRGVAATLDDYAFMLRALIEVYEASFSPAYIRTAVDLARDMIAR
                     YLDRGGFFFTPDDTDLPVRQKTVYDGAVPSGNGVAMHALFVLGRMTANLELEAVADGV
                     LRAFAGAVRKAPAAAPGFLTGLLFMRGPGFEVIIAGVPGAEDTRALVGAVRSRYTPEA
                     VVIFRPSDEEEPDITRVAGFTRDVVAIGGKATAYVCINYACDIPTTEPAEMLRLLASR
                     ERPPEPVI"
     gene            complement(3237..3539)
                     /locus_tag="PLI76_08500"
     CDS             complement(3237..3539)
                     /locus_tag="PLI76_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOI62112.1"
                     /translation="MAALRVAPYVCAYSDENEENLHIEIELPGVEKKDITFKMQETSF
                     SIDASRGDVRYVGTYAVGCPVDTNRAKATFRNGLLTVDVPYVQPAAEETKEIPIAE"
     gene            3635..4018
                     /locus_tag="PLI76_08505"
     CDS             3635..4018
                     /locus_tag="PLI76_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62113.1"
                     /translation="MSERFIFTRHRTDCYHCGEDADQIIKAVSAQAQVVCSACGATRI
                     FVPRFEDTGAVGTCTPIGRYDVWEVRTDAPCKNCGVTGPHDLAVGSGHMTTRCRNCGY
                     AHFYRFNLEYIAQCPLADEEVSVPP"
     gene            complement(4002..4247)
                     /locus_tag="PLI76_08510"
     CDS             complement(4002..4247)
                     /locus_tag="PLI76_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOI62114.1"
                     /translation="MRERLALSRPREAASGKTGSWRTFRPVVDKELCNACGLCAQYCP
                     DGVIDEELNIDLDYCKGCGICANECPKQAIAMVREER"
     gene            complement(4244..4771)
                     /locus_tag="PLI76_08515"
     CDS             complement(4244..4771)
                     /locus_tag="PLI76_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOI62115.1"
                     /translation="MYEIRIHSRGGQGGVTAAKLLALAAFRDGKYATACPFYGAERRG
                     APVVSFVRIDGEPIKIYSQIHEPDLVIVLDTSIMDVVDVLQGLKAGGTVLLNSAHPIT
                     GCNGASLHVDLTGIALAENLVVAGSPILNTPVLGALAKMGIVTLPSAEQAIREMFADE
                     RNVKAARAAYEELKA"
     gene            complement(4764..5876)
                     /locus_tag="PLI76_08520"
     CDS             complement(4764..5876)
                     /locus_tag="PLI76_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOI62116.1"
                     /translation="MLTIATGNKAVAAAVKAARPGVVAAYPITPQTEIVEQIAEYVTD
                     GDLESRYVPVESEHSAMAACIGASVAGVRTFTATSSHGLLYMHEMVHWAAGARLPVVM
                     ANVNRALGPGWNTWAEHTDAFSQRDTGWLQVFVGTVQEAYDATLMAFKIAEDERVLLP
                     VMVNLDGFSLSHITQALDTVDIGDFLPPCHLPHAIDTENPLGYGPMTGPADYFRFRWD
                     IERSMRDARGVIAEVEGEFCERFGRSYGPTEDYRCEDADVVVVAMGTLGKEAEVAIDR
                     LRDEGIKAGSMRLRWFRPFPDLDLAGRDVVVIDRDYSFGFGGVVAHSIRSKAGVSPYS
                     VIAGLGGQEVTYNDIADFVRDRRPGEETWFGVNDHV"
     gene            complement(5870..6751)
                     /locus_tag="PLI76_08525"
     CDS             complement(5870..6751)
                     /locus_tag="PLI76_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOI62117.1"
                     /translation="MSTIPEEEYIVKCTSACAGCSSSLCLRYVLKAAGPDSVLVVPAC
                     CTSVIQGIYPGTAMNVPVYNIAFAAAAACASGMSEAFRALGRKTNVIAYAGDGGTADI
                     GVQALSGALERGTDFLYICYDNEAYGNTGMQRSGATPLGARTTTTPGGKPTAKKDLDR
                     IVEAHNPPYMATACSAYPLDLYKKVKKALSIPGPKFIHILAPCPPGWRYPTEKTVEMG
                     KLAVKTGTWILYEREYGRLTINGPSKTAMRRPAPLEDYIKGQGRFKNLSPEILDRMRQ
                     QIERNVQRLAREEAGAC"
     gene            complement(6842..>7144)
                     /locus_tag="PLI76_08530"
     CDS             complement(6842..>7144)
                     /locus_tag="PLI76_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOI62118.1"
                     /translation="GDFVPRPAEDERILSLVDDISRPVADGTLTWSRWFEEVAPAYMR
                     MNLESYRYRVRRNGILPLLEMGAWFCTPFCLRCCAGLVRRRFRREPVRRSGQSDGE"
BASE COUNT         1196 a         2410 c         2270 g         1268 t
ORIGIN      
        1 cccggctggc aagggagttg tcaacctcgt ctaccgtgga gggaaagaga tcgatcccga
       61 tgccggagca gtacgggagg taggcgggaa tccgctcgag gggggcgatc aatcgcgcgc
      121 aagaacctcg agatcactcc cggaaacagc cctgccgcca gcaaacgaac tggagagggt
      181 ggcgcggagg atgttttcgt cctccacgaa cctctccacc gccgcctgca gtcgttgccg
      241 gacgagttcc gtaccaggcc agaagactct cacagagttg aaaaactctt tccgcgccac
      301 tgattgcatc atcccggcga agtagtcggc cggcttcctc cggggaaaac ccttccggga
      361 tatacgccgg cttcctccgg ggtatacagg cggtcgaggc tgcgtgcgac cgaacagagg
      421 ccggctgccc ggagggcaaa gcggccccat gtctctggta gacggccttt atcgcctttt
      481 cggcggctgc tgcgatacga aacatccgct caaagaaccc ggtatcccgg gcctgcccgg
      541 cgggcgagaa gattcgccga accggagccg tccatctccc cggtgccgaa gacaccgcta
      601 tcagacaccc tcccgcccga tgcagaccct gaccggcacc cggcccgtga gcgaatccac
      661 ggccggcgag cgccggtagc cgcggtgcac gaggtcctcg atcgtctctt ctgacgcatc
      721 ggcatccacc ctgacgataa ggcggatctc ctcaaacccc ggccgcacgt cgtcggcaac
      781 cccaaagagc cctctcaggt cgatatcgcc ctcgacgtcg atcgcaatcg cgtgaaggtc
      841 gattccctcc tccgccgcgc tgtaagcgat cgatacggcg acgcaggaca tgagccccgc
      901 gagcaccagc tccatcgggc ttgccccccg gtcggtcccg ccgagggcgg ccggctcgtc
      961 ggccgggatg acgaagtttc gtacgatcat cgtcgagtgc gcaccgccgt tccagttcag
     1021 gaggccggag acggtcttct gccccacggc cgcatccttt cgcatcgccc ccgcaagggc
     1081 ggcaaacgtt ttcgcgttca tatcaccggc tccggcggcc tctcccgcga cgcaaggagc
     1141 cggagcatct cggccggctc ggtcgtcggt atgtcgcagg cgtagttgat gcagacgtac
     1201 gccgtcgcct tccccccgat agccacgacg tcccgggtga acccggcaac ccgcgtgata
     1261 tcaggctctt cctcatcgga gggccggaat atgacgacgg cctcgggcgt gtagcgcgat
     1321 cggacggcgc cgaccagggc gcgcgtatcc tccgcgcccg ggacgccggc gatgatcacc
     1381 tcaaaacccg gcccccgcat gaagaggagg ccggtgagga aaccgggggc ggcggcaggc
     1441 gccttccgga cagcgccggc aaaagcgcgc aaaaccccat ccgccaccgc ctcaagctcg
     1501 aggttcgccg tcatccggcc gaggacgaag agcgcatgca tggccacgcc gttcccggag
     1561 gggaccgccc cgtcgtacac cgtcttctgc cggacgggca gatcggtgtc gtccggcgtg
     1621 aagaagaacc cgccgcggtc caggtagcgg gcgatcatgt cgcgcgcgag atcgaccgcg
     1681 gtcctgatat acgccgggga gaacgatgcc tcgtagacct ctatgagggc gcggagcatg
     1741 aaggcgtagt cgtcgagtgt cgccgcaacg ccccggcagt ggaggagccg cccgtcctcg
     1801 tcgcgcaggt tctcaagtac gaacgcaagc gccttctccg ccgccgccag gtagcccggg
     1861 tcgtcgaagg cccgggcggc cccggcaagg gcggcgatca tgagggcgtt cgtatcggtg
     1921 aggatcttgt cgtcctttgc cgggcggacc cgctgctccc gggcctggaa gagtctctgc
     1981 cgggcaccct cgacgaacca cgcaaggtcc tcctccggca tcgagaactc gtgagcccat
     2041 gacgcgaggg gccggcggcg gcggaggacc tgcctgcccg gtctatccgc gatgccgaat
     2101 atacgggaga accgctcgcc gtcctccccg aggacgtcga gtatctcggc cttcgtccag
     2161 gtatagaact tcccctcctc cccctcgctg tccgcatcct ccgccgagta gaacccgccg
     2221 ccgggaccgg tcatatcccg caggacgtag gcgatcgtct cgcgggcggt ccgggcaaac
     2281 tcctccctcc ccgtcgcaag gtaggcctcc gtatacgcca tggcgaggag cgcctggtcg
     2341 tagagcatct tctcgaagtg cgggacggcc cacgccacgt ccgttgcgta gcggtgaaac
     2401 ccccacccga tctggtcgaa tatgccgccc tgccgcatgg cgtgcagggt cttttcggcc
     2461 atcgcatacg ccggctcctt cccggtcccg tgcgcgtagc ggagcagaaa gatcaggttg
     2521 tgcggggccg gaaaccgggg cgcgtcccca aacccaccgt tctcctcatc gaatatgcgg
     2581 aggaacgtct cgtaggcctc gtcgatcgct gcgtcgggta tatcgccctc cggggagggc
     2641 gcgctctgca gggcgccaaa cacgcagtcg ccggcttcct cgagttcccc tcgccggtcc
     2701 cgccagacct tgcttatccg ggggatgagg tcgaggaggc cggtcatgcc gtagcggctc
     2761 tccttcggta tgtagctcgc cgcaaagaac ggttttttgt ccggcgtcat gaggatggtg
     2821 agcggccatc ccccggaccc ggccagcacg tgggcggccg ccatgtagat ctggtcgata
     2881 tccggccgct cctcgcggtc gaccttgatg cagacgaaga cgtcgttcaa gagctttgca
     2941 accaccgggt cggcaaacga ctccttctcc atgacgtggc accagtggca ggccgagtag
     3001 ccgatggaga ggaataccgg tttgtcctct ttgcgcgcct tcgcaaatgc ctcctccccc
     3061 caggggtgcc agtcgacggg gttatcggcg tgctggagca ggtaggggct ctgttcgtgt
     3121 atcagccggt tcggtggcga tctcccctgc accggcttct ccggggtcat actcctgacg
     3181 tgccggtaca ggctgataaa ggtgacgccg gcggatagaa aagagagggt ttcccctcac
     3241 tccgcgatgg ggatctcttt tgtctcctcc gcggccggct gtacgtaggg aacgtccacg
     3301 gtcaggaggc cgtttcggaa cgtcgctttc gcccggttgg tatcgaccgg gcagccgacc
     3361 gcgtaggtac cgacgtagcg gacgtccccg cgggacgcat cgatggagaa actcgtctcc
     3421 tgcatcttga acgttatatc cttcttctcg acgcccggta gctctatctc gatgtgaagg
     3481 ttctcttcgt tctcatccga gtaagcacag acgtagggtg cgactctcaa cgctgccata
     3541 tcatgacctc gacaccagag gggcggatgt gctatttaat ccttgtctcc tgccgggggc
     3601 ggaggtttta tctcctgctc gtcgagtatc atgcatgagc gagcggttca tcttcacccg
     3661 gcaccgcacc gactgttacc actgcggtga ggacgccgac cagataataa aagccgtatc
     3721 cgcacaggcg caggtggtct gctccgcctg cggtgcgacg cggatattcg tcccccggtt
     3781 cgaggacacc ggggcggtcg ggacctgcac gccgatcggg cgttacgacg tctgggaggt
     3841 caggaccgat gcgccgtgca aaaattgcgg ggttaccggg ccgcacgacc tcgcggtcgg
     3901 gagcggccac atgacgaccc ggtgccgcaa ctgcggttat gcgcacttct accggttcaa
     3961 cctcgagtac atcgcgcaat gccccctggc cgacgaggag gtcagcgttc ctccctgacc
     4021 atagcgatgg cctgcttcgg gcactcgttt gcgcagatgc cgcacccctt gcagtagtcg
     4081 aggtcgatat tcagttcctc gtcgatgacg ccgtccgggc agtactgggc gcagaggccg
     4141 caggcgttgc acagttcttt atccaccacg ggccggaacg tcctccagga acccgtcttc
     4201 cccgacgcgg cctctcttgg cctgctgagc gcaagtctct ccctcatgcc ttcaactcct
     4261 cgtacgccgc cctggcggcc ttcacgttcc gttcatcggc aaacatctcg cgtatcgcct
     4321 gctccgccga cgggagggtg acgatcccca tctttgcaag cgccccgagc accggggtgt
     4381 tgaggatggg gcttcccgcg accaccaggt tctcggcaag cgctatgccg gtcaggtcga
     4441 cgtgtagaga ggccccgttg cacccggtga tcgggtgagc gctgttcaag aggaccgtcc
     4501 cgccggcttt gagcccctgc aggacatcca cgacgtccat aatgctcgta tcgagcacga
     4561 tcacgaggtc cggttcgtgg atctggctgt atatcttgat cggttcgccg tctatccgga
     4621 cgaacgagac gaccggcgcc ccgcgccgct ccgccccgta gaacgggcag gcggtcgcgt
     4681 acttcccgtc ccggaacgcc gcgagggcga ggagctttgc ggcggtgacg ccgccctgcc
     4741 cgccccggga atgaatcctg atctcataca tggtcgttca ccccaaacca ggtctcctcg
     4801 ccgggacgcc ggtcccggac aaagtccgcg atgtcgttgt aagtgacctc ctggccgccg
     4861 agccctgcga tgacgctgta gggcgaaacg ccggccttcg accggatgga atgcgccacc
     4921 acgcccccaa agccgaacga gtagtcgcgg tcgatgacca cgacatcccg gccggcaaga
     4981 tcgaggtcgg ggaagggacg gaaccagcgc aggcgcatcg acccggcctt tatcccctcg
     5041 tcgcggaggc ggtcgatcgc cacctccgcc tccttcccga gcgttcccat ggcgacgacg
     5101 acgacgtcgg cgtcctcgca ccggtaatcc tccgtggggc cgtaggagcg gccgaaccgc
     5161 tcgcaaaact ccccttcgac ctccgcgata acgccccggg catcgcgcat cgaccgctcg
     5221 atgtcccagc ggaaccggaa gtagtccgcc ggcccggtca tggggccgta accgagaggg
     5281 ttctcggtgt cgatcgcgtg ggggaggtgg cagggcggca ggaagtcgcc gatatccacc
     5341 gtgtcgagcg cctgcgttat gtggctgagc gaaaacccgt ccaggttgac cataacggga
     5401 agaaggaccc gctcgtcctc ggctatcttg aacgccatca gggtggcgtc gtaagcctcc
     5461 tggaccgtgc ccacgaagac ctgcagccag cccgtgtcgc gctgggagaa ggcatcggtg
     5521 tgctccgccc aggtgttcca cccgggcccg agggcgcggt tgacgttcgc catcacgacc
     5581 gggaggcgcg cacccgccgc ccagtggacc atctcgtgca tgtagaggag gccgtgcgag
     5641 ctcgtcgccg tgaacgtccg cacaccggcg acactcgccc cgatacaggc agccatggcc
     5701 gaatgctcgc tctccaccgg tacataccgg ctctcaaggt cgccgtcggt gacgtactcg
     5761 gcgatctgct cgacgatctc ggtctggggg gtgatcgggt aggcggcaac gacacccggc
     5821 cgcgcggcct tcaccgcggc cgccaccgcc ttgttgccgg tcgctatcgt caacatgcgc
     5881 cggcctcctc gcgggcaaga cgctggacgt tccgttcgat ctgctggcgc atccggtcga
     5941 ggatctcggg gctcaggttc ttgaaccggc cctggccctt tatgtaatcc tcgagcggcg
     6001 cgggccgcct catcgccgtc ttcgacgggc cgttgatcgt gagtctcccg tactcgcgct
     6061 cgtagaggat ccacgtcccg gtcttcacgg cgagttttcc catctcgacc gtcttctccg
     6121 tggggtagcg ccaccccggc gggcacggtg cgagtatgtg gatgaacttg ggccccggaa
     6181 tcgagagcgc cttcttcacc ttcttgtaga ggtcgagggg gtaggcgctg caggcggtcg
     6241 ccatgtaggg agggttgtgc gcctcgacga tccggtcgag gtccttcttt gccgtgggtt
     6301 tgccgccggg ggtcgtggtg gtgcgcgctc caagcggcgt tgcgcccgac cgctgcatac
     6361 cggtgttgcc gtatgcctcg ttgtcgtagc atatgtagag gaaatcggtt ccgcgctcca
     6421 gggcgccgga gagcgcttgc acgccgatat cggccgttcc gccgtcgccg gcgtaggcga
     6481 tgacgttggt ctttcgcccg agcgcgcgga acgcctcgct catgccggag gcgcaggcag
     6541 cggctgcggc aaacgctatg ttatagacgg gcacgttcat cgccgtgccc gggtagatcc
     6601 cctggatgac gcttgtgcag caggcgggca cgaccaggac cgagtccggg ccggccgctt
     6661 tgagcacgta gcgcaggcag agagatgaac tgcacccggc acaggcggaa gtgcatttga
     6721 cgatatactc ttcctctggt atcgtagaca aatcgtgtac actccttcct tctctatctg
     6781 tcatcaattg taatgagggt tttgcgcccc gggcccgggc ggccggcagc tctccgccac
     6841 ttcactcccc gtcgctctgc ccggagcgcc gcaccggctc ccggcgaaac cgccggcgga
     6901 cgagcccggc gcagcacctc aggcagaacg gcgtgcagaa ccaggccccc atctccagga
     6961 gggggaggat gccgttcctg cgcacccggt agcgataact ctccaggttc atccgcatgt
     7021 aagccggcgc gacctcctcg aaccaccggc tccaggtgag cgtcccatcg gcgaccggcc
     7081 gggatatatc gtcgacgagg gagaggatac gctcgtcctc tgcggggcgc ggcacgaagt
     7141 cgcc
//
LOCUS       DAOSEE010000113         6855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_4030_length_6855_cov_213.068824, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000113 DAOSEE010000000
VERSION     DAOSEE010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6855
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4030_length_6855_cov_213.068824"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="PLI76_08535"
     CDS             <1..957
                     /locus_tag="PLI76_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOI62119.1"
                     /translation="HSYGVYGPLLNGATCLITETTPDYPTPATNWDLIEEYGVTIFYT
                     APTAIRMFMRVGEEWPNRYNLSSLRILGSVGEPLNPEAFEWFYQTIGKGRCPIVDTWW
                     QTETGMQMLTTTIGEPMRPGFVGKPIPGVVADVVDKTGKPVPPGTGGLLVIKEPWPSM
                     MRTIWNDDERYCKYWDTIPGCYTAADLAVKDEDGYIMVIGRSDDLIVVAGHNIGTAEV
                     ESALVSHEAVAEAAVIGKPDALKGNTIKAFVILRDGRQPGDKLKNDLTYHVRLTLGPI
                     AIPSEIEFVRSLPKTRSGKIMRRVLKAQELGMDPGDISTLEE"
     gene            1000..1563
                     /locus_tag="PLI76_08540"
     CDS             1000..1563
                     /locus_tag="PLI76_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TATA-box-binding protein"
                     /protein_id="HOI62120.1"
                     /translation="MNERRPEESLKIENIVASAKVTDSLDLPSLASQLKDAEYNKKRF
                     PGVVLRMQDPKIAALVFGSGKVVLTGAKSIDSLSRGLDILADQLRALGIDIPEDLTYK
                     IQNIVTSADLGTPINLNKIAVGFNLDKIEYEPEQFPGLVYRLDDPKVVVLLFGSGKLI
                     ITGGKVPEDAKRAVQRILSELSGLGLI"
     gene            1913..2293
                     /locus_tag="PLI76_08545"
     CDS             1913..2293
                     /locus_tag="PLI76_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HOI62121.1"
                     /translation="MRSDKVRYFTPRDEELAELLMGIGIKRNVSKVLVYLANIDEATS
                     RDIERGTDLRQPEVSIAMRYLKECDWIDTRESKSESKGRPVKIYALSRPITEIMDTIE
                     KEKKKEARHQLDLIQKMRECISMQ"
     gene            complement(2282..2968)
                     /gene="rpiA"
                     /locus_tag="PLI76_08550"
     CDS             complement(2282..2968)
                     /gene="rpiA"
                     /locus_tag="PLI76_08550"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HOI62122.1"
                     /translation="MTAEPKRNAGYRAAAMVEDGMAVGLGTGSTVFFAMERLGERIAG
                     EGLSFTGIPTSYQAAIRARRYGIPLAGLDEYPELDVAIDGADQVDPALNLIKGRGAAH
                     LREKCVCDAAREVIIVVDPSKTVDILSAPVPVEVLPFALAGVSRRLSLLGAAPVLREG
                     VKKDGPVVTDNGNFILDCDFGAIGEPEGLETAIAAIPGVLECGLFTAYTEKITVIVGE
                     KIGCRVVSLH"
     gene            complement(2965..3762)
                     /gene="surE"
                     /locus_tag="PLI76_08555"
     CDS             complement(2965..3762)
                     /gene="surE"
                     /locus_tag="PLI76_08555"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOI62123.1"
                     /translation="MKPKIMLTNDDGITSAGLWAAYEALAPIADVTVVAPATQQSAVG
                     RSISIFEPIRATKVLMNGVPAYSVGGKPTDAVIIGLFALHLNPDVVVSGINIGENLSY
                     ESIMTSGTVGAALEASNQGVPSLAFSLQVEDQGDKFDDPSQIRDRFFDAKRVVREVCE
                     RVLANGFPGNAHVINVNIPTPVRGGYEITRLAEKLFYTGVEERFDPRGRPYYWIDGPL
                     HEDAEEGTDVHAVQKGNVSITPITLDCTAFGARDELRGIFDGMVSRS"
     gene            3845..4732
                     /locus_tag="PLI76_08560"
     CDS             3845..4732
                     /locus_tag="PLI76_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOI62124.1"
                     /translation="MKVFLAEYSICNDPGLAPEGAAMLAVLSGSFERCGYDVVTPEGP
                     DLEDGIRRLAPGCDAGLVITPDRLLFRYTHLLEQFTHNIGCGSMNAAISANKQRAGAI
                     LARHGIAVPPAAGSGRRVVKPVMGCGAQDVRLTDGEPGAGEFAEAYIEGEALSVSLVG
                     SRVTGNACEYYSGNPPLLLAVNRQDIAVADDGSFQYLGGETPIHPDREEEIVDLAVRA
                     MNVLGCQGYAGIDVIVGDEIYVVDVNPRPTTSLVGIAAVMEEEIADILIRASHGEAPA
                     DVHLSGRVRFDKDGRIARV"
     gene            4729..5655
                     /locus_tag="PLI76_08565"
     CDS             4729..5655
                     /locus_tag="PLI76_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HOI62125.1"
                     /translation="MIGIDIGGANLKIVDDAGVHIHYCPLWQGAPLAGLLEPYAGSAA
                     VVMSGELADCFSTKIEGIRWIVGTVREAIPDAAFYGTDAAFHTRPVPGLAAANWLASA
                     DYLREEYADAVLLDVGSTTADVIPLTRFEDLKGLTDTRRLQKQYLVYTGMLRTNVATL
                     LSSVTLDGTVTPVSTEYFAASADAHLVLGHIAPADYTCPAPDNGEKTVDAALRRLARV
                     VCADIDEIGKSGAHQVARQFWEVQRRVIGRAVGRALFQGDAERVITAGIGADLFAREL
                     GCATLAQEIGEVSDALPAYAVREVALRRAACD"
     gene            complement(5733..6380)
                     /locus_tag="PLI76_08570"
     CDS             complement(5733..6380)
                     /locus_tag="PLI76_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62126.1"
                     /translation="MDAHTFRTAGQESVTIAKKTLPVPPMLADLVGNALTAMDGVRFT
                     HLDACPSCGGQVTGHDLRPKRFAVILEEGEERTIRVFVKRFYCRQCGSLCYADAPFYP
                     DTRLASPIVDLCLLLSQRMPFNQVAEHLRALGIVVDRGTIRNYASRDFDAIPATELFG
                     LLLPLSLLNLAISTLGREGCPVIGAEALAACGLPPADRAAPRGRGLSEERYQRYN"
     gene            6623..>6855
                     /locus_tag="PLI76_08575"
     CDS             6623..>6855
                     /locus_tag="PLI76_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HOI62127.1"
                     /translation="MTLQYVATTCPYCGTGCSFNLVVQDGKVVGTAPYRRSPVNEGKV
                     CPKGTYAHEFVNSPDRLTKPLIKKDGKFVEATW"
BASE COUNT         1319 a         2181 c         2128 g         1227 t
ORIGIN      
        1 cacagctacg gcgtctacgg cccgctcctg aacggcgcca cctgcctcat cacggagacg
       61 acccccgatt acccgacccc ggccaccaac tgggacctca tcgaggagta cggcgtcacg
      121 atcttctaca ccgccccgac cgccatccgc atgttcatgc gggtaggaga agagtggccg
      181 aaccgctaca acctctcgtc gctccgcatc ctcggatcgg tcggcgaacc gctcaacccc
      241 gaggcgttcg agtggttcta ccaaaccatc gggaaaggcc ggtgccccat cgtggacacc
      301 tggtggcaga ccgagaccgg tatgcagatg ctgaccacga cgatcggtga accgatgcgc
      361 cccgggttcg tcggaaaacc gataccgggc gtcgtcgcgg atgtcgtcga caaaacgggc
      421 aaaccggtcc cgccgggcac cggcggcctc ctggtcatca aagagccctg gccgtccatg
      481 atgcggacga tctggaacga cgacgaacgc tactgcaagt actgggacac catcccgggc
      541 tgctacacgg cagccgacct cgcggtgaag gacgaggacg gttacatcat ggtcatcggg
      601 cggtccgacg acctgatcgt cgtcgccggt cacaacatcg gcaccgcgga ggtcgagagc
      661 gccctcgtct cccacgaggc cgtggcggag gctgccgtca tcggaaaacc cgacgctctg
      721 aaagggaaca ccatcaaggc cttcgtcata ctcagggacg gccgccagcc cggtgacaag
      781 ctgaagaacg acctcaccta ccacgtccgg ttgacgctcg gccccatcgc cataccctcc
      841 gagatcgagt tcgtccggtc gctccccaaa acccggagcg gcaagatcat gaggcgggtc
      901 ttaaaggcgc aggaactggg tatggacccc ggagacatct cgaccctgga agagtagaca
      961 accaacccct ttatatactc ttttatatat gttataaata tgaatgagcg tagaccagag
     1021 gagtccctca agatagaaaa tatcgttgct tcggcgaaag tgacggattc tcttgatctc
     1081 ccctcccttg cctctcagct gaaggacgcg gaatataata aaaaacggtt ccccggtgtc
     1141 gttcttcgta tgcaggaccc gaagatcgcc gcgctcgtct tcggttccgg caaggtcgtc
     1201 ctgacgggcg caaagagcat cgacagcctc tcgagggggc tcgatatact cgccgaccag
     1261 ttgcgggcgc tcggcatcga tatacccgaa gacctgacct acaagatcca gaacatcgtc
     1321 acgtccgccg acctcgggac gccgatcaac ttgaacaaga tcgccgtggg gttcaacctg
     1381 gacaagatcg agtacgaacc cgagcagttc cccggcctcg tctaccgcct cgacgacccg
     1441 aaggtggtcg tcctcctctt cgggtccggc aaactgatca tcacgggcgg caaggtgccc
     1501 gaggatgcga agcgtgcggt gcagcggatc ctctccgagc tctccggtct cgggctcatc
     1561 tgaactctgg caactactct tttccggatt ccgggaatgc gttctcgggt ctctgatccg
     1621 tatccccctt ctcacacggt gccgccgcaa aatccgctcc cggccagcac gtgccgcccc
     1681 ctgcccggga gacgcctctc tccggcgtat gttggtcggg gttgccggcc acgccctgtc
     1741 cccgggtatt ccccacactg cccggcaaag cgcgatccgt cagagaaaga tatcatcaac
     1801 ccgatgccct atcggtcttt tcaggtgcag cgtaatcgcc ggatctccca ttagaataat
     1861 aatattttta tagtcagatt taaaaccata gatactacct gatggtgaac ggatgaggtc
     1921 agacaaggta cgatacttta cgccacgcga cgaagaactc gccgagctcc tcatgggcat
     1981 cggtatcaag agaaatgtct ccaaagtgct cgtatatctt gcaaacatag atgaagcgac
     2041 gtcccgcgat atcgagcgcg gcaccgatct ccgccagccc gaggtcagca tcgccatgcg
     2101 ctacctcaag gaatgcgact ggatcgatac ccgcgagagc aagtcggaga gcaaggggcg
     2161 gccggtgaag atatacgcgc tctcccgtcc cataaccgag atcatggata cgatcgagaa
     2221 ggagaaaaag aaagaagccc ggcaccagct agacctcatc cagaagatgc gggaatgtat
     2281 atcaatgcag tgagacgacg cggcaaccga ttttttcgcc gacgataacc gttatctttt
     2341 ctgtatacgc cgtaaaaaga ccgcactcca gaacaccggg gatggcggct atcgctgttt
     2401 caagcccctc gggctccccg atcgccccga agtcgcagtc gagtatgaag ttgccgttgt
     2461 cggtgacgac cgggccgtct ttcttcaccc cttcccggag gaccggcgcg gccccgagca
     2521 gggagaggcg ccgggagacg cccgcgaggg caaacgggag cacctcgacc gggacgggcg
     2581 cgctcaggat atcgaccgtc ttggaggggt cgacgacgat gatcacctca cgggccgcat
     2641 cgcagacgca cttctcccgg aggtgggccg ccccgcgccc ctttatcagg ttcagggccg
     2701 gatcgacctg gtcggccccg tcgatggcga cgtcgagctc cgggtactca tcgaggccgg
     2761 cgagcggtat gccgtagcgg cgcgcccgga tggccgcctg gtaggatgtc ggtatgccgg
     2821 tgaaagagag cccctccccg gctatccgct cgcccaggcg ctccatcgca aagaagaccg
     2881 tcgaccctgt cccgaggccg acggccatac cgtcctcgac catcgcggcc gcacgatacc
     2941 ctgcgttccg cttcggttct gccgtcacga cctgctcacc ataccgtcga atatgccccg
     3001 gagctcatcc ctggcgccga acgccgtgca gtcgagcgtg atgggcgtga tggagacgtt
     3061 gcccttctgc accgcatgca cgtcggtccc ctcttccgca tcctcgtgca gcggcccgtc
     3121 tatccagtag tacgggcgcc cgcgggggtc gaagcgctcc tcgacgccgg tgtagaagag
     3181 tttctcggca aggcgggtga tctcgtagcc gccgcgcacc ggtgtgggta tgttcacgtt
     3241 gatgacgtga gcgttcccgg ggaacccgtt tgcgaggacc cgctcgcaga cctcgcggac
     3301 cacccttttc gcgtcaaaga acctgtccct gatctgggat gggtcgtcga acttgtcgcc
     3361 ctggtcctcc acctggagag agaaagcaag ggaaggcacc ccctggttgg atgcctcgag
     3421 ggcggcgccg acggtccccg acgtcatgat ggactcgtac gagaggttct caccgatgtt
     3481 gatgccgctg acaacgacat ccgggttcag gtgcagggca aacagaccga tgataacggc
     3541 atcggtcggt ttcccgccga ccgagtaggc cggcacgccg ttcatcagca ccttcgtcgc
     3601 ccggatcggc tcaaagatcg atatcgatcg ccccacggcg ctctgctggg tggcgggcgc
     3661 gacgaccgtg acgtcggcga tcggcgcgag ggcctcatag gccgcccaga gtcccgcgga
     3721 cgtgatcccg tcgtcgttgg tgagcataat ctttggcttc atccttatct ataggattgt
     3781 tcccccgtat caaatacttg cccgatcgtc agggtcaagt gcctgagagg caacctttac
     3841 tgcgatgaag gtttttctcg ccgaatactc catctgcaac gatcccggcc ttgcgcccga
     3901 gggtgccgcc atgctcgccg tgctcagcgg aagttttgaa cggtgcggct acgacgtggt
     3961 gacgccggag ggccccgacc tcgaggacgg catacggagg ctcgcgccgg ggtgcgatgc
     4021 cggtctggtc atcacgccgg accgcctcct ctttcggtat acgcacctcc ttgagcagtt
     4081 cacccacaac atcgggtgcg ggagcatgaa tgcggcgatc tctgcaaaca aacagcgggc
     4141 cggagcgatc ctcgcccggc acggcatagc ggtcccgccc gctgccggca gcgggagacg
     4201 ggtcgtcaaa ccggtgatgg gctgcggggc acaggatgtc aggctgacgg acggggagcc
     4261 gggcgccggg gagtttgccg aggcttacat cgagggggag gcgctgagcg tgagcctcgt
     4321 ggggagccgg gtgaccggca acgcctgcga gtactactcg ggcaacccgc cgctcctgct
     4381 tgccgtcaac cggcaggata tcgccgtcgc ggacgacggg agtttccagt acctcggggg
     4441 agagacgccc atccaccctg atcgggaaga ggagatcgtc gatctcgccg ttcgggcgat
     4501 gaacgtcctc gggtgtcagg ggtatgccgg catcgacgtc atcgtcgggg acgagatcta
     4561 cgtggtcgac gtcaacccgc gccccacgac cagcctggtc ggcatcgcgg cggtcatgga
     4621 ggaggagatc gccgacatac tcatcagggc gtcgcacggc gaggcgccgg cggacgtgca
     4681 cctctccggg agggtgcggt tcgataagga cgggaggatc gcccgggtat gatcggcatc
     4741 gacataggcg gtgcaaacct caagatcgtc gacgacgccg gggtgcacat ccactactgc
     4801 ccgctctggc agggcgcgcc gctcgccggc ctcctcgagc cctacgccgg ctccgccgcc
     4861 gtggtgatga gcggggagct cgcagactgt ttttcgacca agatcgaggg catccggtgg
     4921 atcgtcggga ccgtccggga ggccataccc gatgccgcct tctacggcac cgatgcggcg
     4981 ttccataccc ggccggtccc gggccttgcc gccgcaaact ggcttgcgtc cgccgactac
     5041 ctgcgggagg agtatgccga tgcggtcctc ctcgacgtgg ggagcaccac cgccgacgtc
     5101 atccccctga cccgctttga ggacctcaaa ggcctgaccg atacccggcg gctgcagaag
     5161 cagtacctcg tctacaccgg catgctcagg acgaacgtcg cgacgctcct ctcgtcggtc
     5221 acgctcgacg ggacggtgac cccggtgagc accgagtact tcgcggcgag cgcggacgcg
     5281 cacctcgtcc tcggccacat cgcccctgcg gactacacct gcccggcgcc ggataacggc
     5341 gagaagaccg tcgatgccgc gctccggcgg cttgcccggg tcgtctgcgc cgatatcgac
     5401 gagataggga agtccggggc tcaccaggtc gcccggcagt tctgggaggt ccagcggagg
     5461 gtgatcgggc gcgcggtggg gcgcgcactc ttccagggcg atgccgaacg agtgatcacg
     5521 gccggcatcg gggcggacct ctttgcccgg gaactgggct gtgccacgct ggcgcaggag
     5581 atcggggagg tctcggatgc cctgccggcg tacgctgtca gggaggtggc gttacggcga
     5641 gccgcctgcg actgaccctc cttttgcttg cggtgtccgt cgccgtcacg gcagtctcgt
     5701 tcgccctcgg gtttccgttc ttcttcctct ttctagttat accgctgata ccgttcttcg
     5761 gaaagccccc tgccgcgagg cgctgcccgg tctgcgggtg ggagaccaca ggcggcgagc
     5821 gcttctgccc ctatgacggg acacccctcg cgcccgaggg tggatatggc gaggttcaag
     5881 agcgagaggg gtagaagaag gccgaagagt tcggttgccg gtatcgcgtc gaagtcgcgt
     5941 gaggcgtagt tccggatcgt accgcggtcc acgacgatcc ccagcgcgcg gaggtgttcg
     6001 gcaacctggt tgaagggcat ccgctgcgag aggaggaggc aaaggtcgac gatgggcgac
     6061 gcgaggcggg tgtcggggta gaaaggtgca tccgcgtagc agagcgaacc gcactggcgg
     6121 cagtaaaacc gcttcacaaa gaccctgatc gtccgctcct ctccctcctc aagtatgacg
     6181 gcaaatcgtt tcggccgcag gtcgtgtccg gtcacctggc cgccgcacga cggacaggcg
     6241 tcgaggtggg tgaatctcac gccgtccatc gctgtcaggg cgttgcccac gagatcggcg
     6301 agcatcggcg gaaccggcag tgttttcttt gcgatggtaa cgctctcctg tccagctgta
     6361 cgaaaagtgt gggcgtccac cgataataac atattcctcc ggccggcagt attccggccc
     6421 ccggttgaac acatatgtct gaccggtgcg ggtacataaa cccgggatct atgggaggtc
     6481 ctccgcgtaa acgtcagaca aagtcgtgta atccgttttc gacctggcgg gactgttcag
     6541 ggggcccggg cagatctggt taaccactat aatttaagta gcttaacatc agatattcgg
     6601 tcgccagaga ggcgatagat gcatgacgtt acagtatgtt gctacaacgt gcccctactg
     6661 cggtacagga tgcagtttca acctcgtcgt ccaggacggc aaggtggttg ggaccgcacc
     6721 gtaccgccgc tcacccgtca acgagggaaa ggtctgcccg aaaggcacct acgcccacga
     6781 gttcgtgaac agccccgatc gtctcaccaa gccgctcatc aagaaggacg gcaagtttgt
     6841 cgaggcgacc tggga
//
LOCUS       DAOSEE010000114         6844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_4040_length_6844_cov_244.746944, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000114 DAOSEE010000000
VERSION     DAOSEE010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6844
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4040_length_6844_cov_244.746944"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /gene="rimI"
                     /locus_tag="PLI76_08580"
     CDS             complement(<1..447)
                     /gene="rimI"
                     /locus_tag="PLI76_08580"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOI62128.1"
                     /translation="MPLQLTIRRAQPADIPQIVAIERMAFTDPWDERTLCESLAYFPE
                     TFFVARNNGDMAGFVAAGLEDTGEDIYGHIMNLAVAPGYRRQGVGQHLICRLEQEFML
                     SGANAAQLEVRISNTGAQGFYHRLGYREVFRIASYYANGEDALIMMK"
     gene            540..1802
                     /locus_tag="PLI76_08585"
     CDS             540..1802
                     /locus_tag="PLI76_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOI62129.1"
                     /translation="MVQIYRFAAVRPERRYSGDIPSVPYDVVTAEEARECIEKNPLSF
                     LRVSRPDAELPDLPPHDDRVYERAREVFYGLLADGRLQRDPEPGMYVYRAVQDGEEFV
                     GLVCCVATEEYENRQIRRHELTRYDKEEDRTRHIDAVGANTGLVFLLYRDPDDIYTAI
                     RSLIDGAEPDGSTTTVSGVLHQVYRITDEAALSHLEGLFATVPEAYIADGHHRAKSAV
                     NVAERRREEGRFTEEAGRFMVVLFARDHVRIHGYSRLVTDLGGLTLPGFIDVLLRAGW
                     SIRPHGKVDASEYQVRPLATGDEPLHVMHFYMQGTWYEVSRPVDNPADLIGSLDVSVL
                     QKEVLEGMLGINDPRGDPRLHYMGGAKPLTGLERLVDSGEYALAVAMQPVDVETVLAI
                     ADADGVMPPKSTWFEPKLLSGLVVHTID"
     gene            1849..2721
                     /gene="hisG"
                     /locus_tag="PLI76_08590"
     CDS             1849..2721
                     /gene="hisG"
                     /locus_tag="PLI76_08590"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOI62130.1"
                     /translation="MITIALPKGSLEAQTLQLFKEADLEVRRTDRDYNPRINDPRIGK
                     VKILRPQEIPLYVQMGYFDIGISGLDWVQESGADVREIANLSYSKTGDGNVKIVVAVH
                     RDEPIDSIAAIRPDSRVTTEYPRITERFFKENGVPVRLFASYGASEAKVPDLMDVVVD
                     LTETGSTLKKNGLKIVGQIMESHTALLANHEAFADPEKRREIEEITTLLLGVIEARHQ
                     VLLTMNVPSGALDRVIDVLPAMKKPTVGRLHGIDYFSIQTVVQKGLVNGLIPHLKAAG
                     AEDILEIPITKIVR"
     gene            complement(2731..3387)
                     /locus_tag="PLI76_08595"
     CDS             complement(2731..3387)
                     /locus_tag="PLI76_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HOI62131.1"
                     /translation="MQGKHMCTTTPPGMPGEAHDRFAEAARFHGHICPGLALGYRAAG
                     IALERLRSGRAEDEELVTIAETDACGVDAIQVLTGCTAGKGNLLFKDHGKHAFTFINR
                     VTGDAVRVVGNPSFDTDSLDPGLASLRTRVMQGQASGEELAEYRRRIDRIVEKILDLP
                     AETLFTIRKVDVDMPERARIFKSVPCAKCGELTAESRVRVEDGKFVCRSCSKEYTRRW
                     "
     gene            3621..4184
                     /locus_tag="PLI76_08600"
     CDS             3621..4184
                     /locus_tag="PLI76_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62132.1"
                     /translation="MRTSGSVPALIATVCFFCATCGCLGAAPLPTGGGITPIDLPEPP
                     AASTFADALAELDLLGAEGGLNVTGASVRQVLGTGVEIDGQAASWALGLQDGEEIRWL
                     TYGPAGWQEISFSAPLPADEVNVTGVLSPEDLLRGREDMFRPVMERLGADTVDLALAG
                     GVYSVTIRSDAGMETLSFEADTGEVIA"
     gene            4190..>5008
                     /locus_tag="PLI76_08605"
     CDS             4190..>5008
                     /locus_tag="PLI76_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62133.1"
                     /translation="MADDGVLSLDFLAGFTIFMLALVMVLSMVPGLLAGLESSGIDYD
                     AVAYRTGVILVEDPGWPAYPPWELYDRDNKEEIQRMGLAVSKETPNILLSTKVERFFE
                     ESFFTSEDYRSKVIFGDIPYSYNISLRSETYNYNTGDPLPPGYGYIRRVVKIKEPGVA
                     EIKSPGPEYVIPDPSAPEWNSTARNFTVRLNFSQLLDPGRGPAYRIDPRTEPVNITIT
                     NFGEYRNITGPSVNATLHNVTFWKMDPTRPNPRFTRIPFSYDTIDANLYTLHIND"
     assembly_gap    5009..5108
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5109..5344
                     /locus_tag="PLI76_08610"
     CDS             <5109..5344
                     /locus_tag="PLI76_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62134.1"
                     /translation="AVPHSLQPDVPVNSSISLVLKPAATSLFTLDQNSILDIRFNFED
                     DPPRTNITGTHPYDYTNVSHAGLKTAVLEVAIW"
     gene            5338..5859
                     /locus_tag="PLI76_08615"
     CDS             5338..5859
                     /locus_tag="PLI76_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62135.1"
                     /translation="MVNDAGQLYTMEGVAAGVIMLLAAYIVVSTTSIYTTGDTHIPDM
                     QLEQLGSDVLAMMDTPDTNGTTSALETFVSTNNGTGFNSTFLGYCNATTRGTLDNLHM
                     SANITYRDANANKINEYPFVAPDNTWTGRESAVRATRWVQLPKLSPKPVGMPGDMRTD
                     PQAVLVEVLLWRA"
     gene            5856..6230
                     /locus_tag="PLI76_08620"
     CDS             5856..6230
                     /locus_tag="PLI76_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62136.1"
                     /translation="MNEEGQWIVLMGLLVAVALFFLALIINQSALVGQTTAEGVLEFP
                     KNDIRDLREAVFDYVDAAGALDPIERVDIEKDIITISLERKNAVVQFGNGTPQDVSGR
                     WLRPVDIHYNNGVTVYNETVYY"
     gene            6211..6669
                     /locus_tag="PLI76_08625"
     CDS             6211..6669
                     /locus_tag="PLI76_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62137.1"
                     /translation="MRPCIIEPMDEEGVTRLMEYIIITGVLLLLMIVMMFSVNASIME
                     GPANKLRYHAFVDIGNGVSTRIVDLYVIAPGNGTIETKFDIPDDVAGKGYFVDVWMEG
                     ENRASQAIEVQGGAIKSKVAIAGIGATRGVIGNTTGAGWNMIQYDSKGFL"
BASE COUNT         1323 a         2062 c         2097 g         1262 t
ORIGIN      
        1 cttcatcatg ataagggcat cttcaccgtt cgcgtaatac gatgcgatcc ggaagacctc
       61 ccggtagccg aggcggtggt agaacccctg cgccccggtg ttgctgatcc ggacctcaag
      121 ctgggcggcg ttcgcacccg agagcatgaa ctcctgttcc aggcggcaga taaggtgctg
      181 cccgactccc tggcgccggt accccggtgc gaccgcaagg ttcatgatgt gcccgtatat
      241 gtcttcaccc gtatcttcaa ggcccgcggc gacgaacccg gccatatccc cgttgttccg
      301 tgccacaaag aatgtctccg ggaagtaggc gagcgattcg cagagcgttc tctcatccca
      361 ggggtcggtg aacgccatcc tctcgatggc aactatctgt ggaatatctg caggctgcgc
      421 cctgcgaatg gtgagttgta gcggcatcat cgttaccggt cacccccctt cctggagtgc
      481 cctacctaat atagtgtcag gagacaaaat agtaggcact cacgagtgtg gcggttttca
      541 tggtccagat ctatcgtttt gcagcggtcc gtcctgagcg gcggtactcc ggggatatac
      601 cttccgtgcc gtacgatgtg gtgacggcag aggaggcccg ggagtgtatc gagaagaacc
      661 ccctgagttt cctgcgggtc agccggccgg acgcagagct ccccgatctc cctccgcacg
      721 acgatcgggt ctacgagcgg gcacgggagg tcttttacgg cctgctcgcc gatggccggc
      781 tgcagaggga cccggagccg ggcatgtacg tctaccgggc cgtccaggac ggcgaggagt
      841 tcgtcgggct ggtctgctgc gtggcgacgg aggagtacga gaaccggcag atccggcggc
      901 acgaactcac ccggtacgac aaggaagagg accggacccg gcacatcgat gcggtggggg
      961 caaacaccgg cctcgtcttc ctcctttatc gtgacccgga cgatatctac accgccatcc
     1021 gctccctcat cgacggggcg gagccggacg ggagcaccac gaccgtgtcg ggggtgctcc
     1081 accaggtcta ccggatcacc gatgaggcgg ccctctccca ccttgaaggg ctctttgcaa
     1141 ccgtgccgga ggcctacata gcagacgggc accaccgggc caaatcggcg gtgaacgtgg
     1201 ccgagcggcg ccgggaggag ggcaggttca ccgaggaggc ggggaggttc atggtcgtcc
     1261 tctttgcccg ggaccacgtt cgcatccacg gctacagccg gctcgtcacc gatctcggcg
     1321 gtctcaccct ccccgggttc atcgatgtgc tcctgcgggc aggctggagc atccgcccgc
     1381 acgggaaggt cgacgcctcg gagtaccagg tccggccgct cgcgaccggg gatgaaccgc
     1441 tccacgtcat gcacttctac atgcagggga cctggtacga ggtctcccgg ccggtcgata
     1501 accccgccga cctgatagga tcgctcgacg tctcggtgct ccagaaggag gtcctcgaag
     1561 ggatgctcgg catcaacgat ccccggggag acccgaggct ccactacatg ggcggggcaa
     1621 aacccctcac cgggcttgag cgtctggtgg attccggcga atatgcgctt gcggtggcga
     1681 tgcagccggt agatgtcgag acggtgcttg ctatcgccga tgccgacggt gtcatgcccc
     1741 cgaagtccac ctggtttgag ccgaaactcc tctcgggcct cgtcgtccac accatcgact
     1801 gagatccgga tcatcacctt taatcccttt tctgaccgat caactcttat gataacgatt
     1861 gccctcccga aggggagcct cgaggcgcag acgctccagc tcttcaagga ggcggacctt
     1921 gaggtcaggc ggaccgaccg cgactacaac ccccgcatca acgatccccg gatcgggaaa
     1981 gtgaagatcc tccgcccgca ggagatcccg ctctacgtcc agatgggcta cttcgatatc
     2041 ggtatatcgg ggcttgactg ggtgcaggag agcggagccg acgtcaggga gatcgcaaac
     2101 ctctcctaca gcaagacggg cgacggaaac gtgaagatcg tggtcgcggt ccaccgcgac
     2161 gagccgatcg acagcatcgc cgccatccgg ccggacagcc gggtgacgac cgagtatccc
     2221 cggatcacgg agaggttctt caaggagaac ggcgtccccg tcaggctttt tgcctcttac
     2281 ggggcatcgg aggcgaaggt gcccgacctg atggacgtcg tcgtcgacct cacggagacc
     2341 gggagtacgc tcaagaagaa cgggttgaag atcgtcgggc agatcatgga gtcgcacacc
     2401 gcgctgcttg caaaccacga ggctttcgcc gacccggaga agcgccggga gatcgaggag
     2461 atcacgaccc tcctcctcgg cgtgatcgag gcgcgccacc aggttctcct gacgatgaac
     2521 gtgccctcgg gggcgcttga ccgcgtcatc gatgtcctcc ccgcgatgaa gaagcccacc
     2581 gtcggccggt tgcacgggat cgactacttc agcatacaga cggtggtgca gaaagggctt
     2641 gtcaacggtc tcatcccgca cctgaaggct gcgggtgccg aggatatact tgagataccg
     2701 ataacgaaga tcgtgcggtg aggggtcccc tcaccagcgc cgggtgtatt cttttgagca
     2761 ggaacggcag acgaatttgc cgtcctcgac ccgcacccgt gactcggcgg tcaattcgcc
     2821 gcattttgcg cacggcaccg acttaaatat ccgggcccgc tccggcatat cgacgtcgac
     2881 ctttcggatg gtgaagaggg tctcggcggg caggtcgagg atcttctcga cgatccggtc
     2941 tatccgcctc cggtactcgg ccagttcctc accggaggcc tggccctgca tgacccgggt
     3001 cctgagcgat gcaaggccgg ggtcgaggga gtcggtatcg aacgacgggt tcccgacgac
     3061 ccgaaccgca tcgccggtca cgcggttgat gaacgtgaag gcgtgtttgc cgtggtcctt
     3121 aaagaggagg tttccttttc cggcggtgca ccccgtcagg acctggatgg catcgacgcc
     3181 gcaggcatct gtctcggcga tggtgacgag ttcttcgtcc tcggcccggc cggagcggag
     3241 ccgttcaaga gcgataccgg ccgcccgata ccccagagca aggccggggc agatgtggcc
     3301 gtggaaccgg gctgcttcgg cgaaccggtc gtgtgcctct cccggcatgc cggggggtgt
     3361 agtagtacac atgtgcttac cttgcatgat atgttgctac taacagagag atataagtta
     3421 cgatgccctg ccggggcctg cgggcagggg ctcctggcat ataaagaccc gggtgctccg
     3481 gatttccagc cctcatttga gacgaaatat ttttgccgcc gggtacctgc acgtttccgg
     3541 tatcgaggga tggtgacgga ttcttgactc cccgcggggg atgattattt ataccccttc
     3601 gtgtataatg agtgccttgc atgagaactt ccggaagcgt cccggccctg attgccacgg
     3661 tctgtttctt ctgtgccacg tgcggttgtc ttggtgcagc cccgctaccg accggcggcg
     3721 gcatcacccc gatcgacctg ccggaaccgc cggcggcctc gaccttcgcc gatgctctcg
     3781 cggagctgga cctgctcggt gccgagggcg gtctgaacgt caccggggca tcggtacgcc
     3841 aggtgctcgg gaccggcgtc gagatcgacg gtcaagccgc ttcctgggcc ctcggcctcc
     3901 aggatggcga ggagatccgg tggctgacct atggccctgc gggctggcag gagatatcgt
     3961 tctcggcccc tctgcccgcg gatgaggtga acgtcaccgg cgtcctctct cccgaagacc
     4021 tccttcgcgg tcgggaggat atgttccggc ccgtgatgga gcggctcggt gccgatacgg
     4081 tcgatcttgc tctcgccggg ggggtgtatt ccgtcaccat ccgctcggat gccgggatgg
     4141 agaccctctc gtttgaggcg gataccgggg aggtgatcgc ataagatcgg tggccgacga
     4201 cggggtgctc tcgcttgact ttctcgcggg gttcaccatc ttcatgctcg ccctggtcat
     4261 ggtgctgagc atggtgccgg ggctccttgc cggccttgag agcagcggca tcgactacga
     4321 cgcggtcgcc taccggacag gggtgatcct cgtcgaggat ccggggtggc cggcctaccc
     4381 gccgtgggag ctctacgacc gggataacaa ggaggagatt cagcggatgg ggcttgccgt
     4441 ctcgaaagag acgccgaata tcctcctctc gacgaaggtc gagagatttt ttgaggaatc
     4501 gttcttcact tccgaggatt accgcagcaa ggtgatcttc ggcgacatcc cctattctta
     4561 caatatctcg ctccggagcg agacatacaa ctacaatacc ggcgaccccc tccctcccgg
     4621 ctacggctac atccgccggg tggtgaagat caaggagccg ggggtggcgg agatcaagag
     4681 ccccgggccg gagtacgtca taccggatcc atctgctccg gagtggaact ccaccgcgag
     4741 aaactttacc gtccggctca acttcagcca gctccttgac ccggggcggg gcccggccta
     4801 ccggatagac ccgaggaccg aaccggtgaa catcacgatc acgaacttcg gagagtatcg
     4861 taacattacc ggaccatccg tgaacgcaac gctccacaac gtcacgttct ggaagatgga
     4921 tccgacacgg ccaaacccgc ggttcactcg catcccgttc tcgtacgata cgatcgacgc
     4981 aaatctctac accctgcaca tcaacgacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnct gcagtcccgc actcgctcca gccggatgtg ccggtcaatt ccagcatctc
     5161 cctcgtcctg aaaccggcgg ccacctcgct cttcaccctc gaccagaaca gcatcctcga
     5221 tatccggttt aacttcgagg acgacccccc gcggaccaac atcaccggca cgcacccgta
     5281 tgactacacg aacgtcagcc acgccggcct caaaaccgcc gtcctggagg tcgccatatg
     5341 gtgaacgatg caggacagct ctacacgatg gagggtgtcg cggccggggt catcatgctg
     5401 ctcgccgcgt acatcgtcgt cagcacgacg agcatctaca ctacgggaga tacgcacatc
     5461 ccggatatgc agctagagca gctcgggagc gacgtgctcg ctatgatgga cacgccggat
     5521 accaatggca caacgagcgc cctggagact tttgtcagca cgaacaacgg taccggtttc
     5581 aacagcacgt tcctgggcta ctgcaacgca acgacaaggg gtacgctcga taacctgcac
     5641 atgagtgcaa acatcaccta ccgggatgca aacgcgaaca agattaatga gtacccgttc
     5701 gttgcacccg acaacacctg gacgggaaga gaatccgccg tgcgggcgac ccggtgggtg
     5761 caactgccaa aattatcccc aaagccggtc ggcatgcctg gtgatatgcg aacagaccct
     5821 caggccgtcc tcgtggaggt gcttttgtgg cgcgcatgaa cgaagaaggc cagtggattg
     5881 tcctgatggg gctcctggtc gccgtggcgc tcttcttcct cgcgctgatc atcaaccagt
     5941 cggcgctcgt cgggcagacg accgcggagg gggtgcttga gttcccgaag aacgatatcc
     6001 gggatctacg agaggcggtg tttgactacg tcgatgctgc tggggcgtta gaccctatag
     6061 aacgtgtgga tattgagaag gatatcatca caatatccct cgaacggaag aacgccgtgg
     6121 tacagttcgg gaacggaacg ccgcaggacg tctccgggcg ctggctccgg ccggtagaca
     6181 tccactacaa caacggggtg acggtgtaca atgagaccgt gtattattga accgatggat
     6241 gaggagggcg tcacccggct tatggagtat atcatcatca ccggcgtgct cctcctcttg
     6301 atgatcgtca tgatgttctc cgtcaacgcc tcgatcatgg aggggccggc gaacaaactc
     6361 cgctaccacg ccttcgtgga catcgggaac ggtgtcagca cccggatcgt ggacctctac
     6421 gtgatcgcgc ccggcaacgg gaccatcgag acgaagttcg acatccccga cgacgtcgcc
     6481 gggaaaggct acttcgtgga cgtgtggatg gagggcgaga accgggcaag ccaggcgata
     6541 gaggtgcagg gcggcgccat caagagcaag gtcgccattg ccggcatcgg ggcaacgagg
     6601 ggtgtcatcg gcaacacgac cggtgcgggg tggaacatga tccagtacga ctcgaagggg
     6661 tttttatgat aaatgagaag gcagtttcgg aggcgatcgg atttatcctg atcctgggga
     6721 tcgtgctcac cggtatcggc ctcgttacgc tctacggcta cccggtgctc gtcaaagagc
     6781 agagcaacgc cgacgtcagg aacatggagc gggcgatgat cgtcatccag aacgacatga
     6841 agag
//
LOCUS       DAOSEE010000115         6839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_4047_length_6839_cov_214.407134, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000115 DAOSEE010000000
VERSION     DAOSEE010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6839
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4047_length_6839_cov_214.407134"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1374)
                     /locus_tag="PLI76_08630"
     CDS             complement(<1..1374)
                     /locus_tag="PLI76_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOI62138.1"
                     /translation="MDRRRDRFFPAFSGKKVLPAANSQFAIIASDLGLIFLFIALVSC
                     TPFLVGFWYGELDLLLPMASAPAGFGILGVWLASVAKAEGEAHLSVALSAVALIWLAA
                     ALIGALPYMFGLGIPYTDSVFEAMSGWTGTGFSLLPSVDTVPRMILFWRSLTQWMGGL
                     GIVAFTIALAGRSGLTRFQLYRSEGRSEAFMPGVVATATEMWRIYLVLTALGIGLVLL
                     SGISLWDAVNIVMTAISTGGFAVHSAGILYYESPLLEMLVVPVMIAGALPFKLYFILY
                     YRRTFEFFRDSQAILLFALILVGCTAITFDLVALSSLAPAEALREGIFMTVSGITCTG
                     FQNANPRDWYGATVMVLTALMLIGGSAGSTAGGIKLGRVILGLETLGWWIRRLRAGWR
                     TITPLRHEGRPILEHLPEYEVSKSMLVVVLFILAVAVATLLLLHLVPQGGFDSTDVIF
                     EVVSAISN"
     gene            1503..1712
                     /locus_tag="PLI76_08635"
     CDS             1503..1712
                     /locus_tag="PLI76_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62139.1"
                     /translation="MTSAERAAVTMGFEEPDRVPLFTIYGAKDLGLSIREYSSKLKYV
                     AAGQMRLLEKFGGIDCPAHQGGVCR"
     gene            2037..2618
                     /locus_tag="PLI76_08640"
     CDS             2037..2618
                     /locus_tag="PLI76_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2121 domain-containing protein"
                     /protein_id="HOI62140.1"
                     /translation="MTLVMAYIGRQGAVMAGDMREIAFQGDDPCIEALERELYSGSIA
                     SDNDLLERAGEIGVAIRVRDDKVKVTEQDGVLVGEVTETAGSTIRQKRLYVTAGSYVI
                     AEIIDSRLRVTQRGQAGNFVVLGNDITKQVAGQCIREAWKGGTVPEAMRVILLTMQMA
                     SGVTASVSRTFMLVHTDRAMNLAGAINRASGGE"
     gene            complement(2684..3001)
                     /gene="eif1A"
                     /locus_tag="PLI76_08645"
     CDS             complement(2684..3001)
                     /gene="eif1A"
                     /locus_tag="PLI76_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor eIF-1A"
                     /protein_id="HOI62141.1"
                     /translation="MTNTRGSQNSGSEEIVRVRLPKKRNRELFGRADLMLGANHIRVR
                     CEDGVTRMGRIKGKIKKRLWIREGDLLIVVPWSFQDEKCDIIYRYIRPQVDWLKKHNY
                     LSQ"
     gene            3553..3813
                     /locus_tag="PLI76_08650"
     CDS             3553..3813
                     /locus_tag="PLI76_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOI62142.1"
                     /translation="MESSKLYVGNLTYSVNEEQLKELFGRYGTVNDVRVIERKGFGFV
                     EMSSPEEAEKAKDALNNTVFEGRTLKIDEARPPRPRSDFRRY"
     gene            4133..4471
                     /locus_tag="PLI76_08655"
     CDS             4133..4471
                     /locus_tag="PLI76_08655"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOI62143.1"
                     /translation="MVIGRDDAYNYYIDSTQKALYACVLSLRKDFTILDFGCGNGRWA
                     FWFAPRVARVIGIDLSPAMVDAARRFASEQGIQNVGFTVPGDGSQFPDDSFDIVNCRP
                     RPHLHRACDG"
     gene            4673..4999
                     /locus_tag="PLI76_08660"
     CDS             4673..4999
                     /locus_tag="PLI76_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HOI62144.1"
                     /translation="MCPAVRDAVNKVVRSMAEERRSVIEEERPSVRGGHASAAQAGSV
                     EELSASAFQSYLHGMDYPGKKQDLINLARKNNAPDAVIQVLEMFNDDKTFQSAADVSQ
                     EFGRVK"
     gene            complement(5077..5161)
                     /locus_tag="PLI76_08665"
     tRNA            complement(5077..5161)
                     /locus_tag="PLI76_08665"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5123..5125),aa:Leu,seq:cag)
     gene            complement(5199..>6839)
                     /locus_tag="PLI76_08670"
     CDS             complement(5199..>6839)
                     /locus_tag="PLI76_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOI62145.1"
                     /translation="VPEYYNFGFDVIDAWAKKDRNKLAMIWTDQKGNEKKYTFFDLMR
                     LSNQAVNICIKYGIKKGDRVMLMLPRAPEWWIFTIALIKIGAVYCPATTMLTAKDLKY
                     RIQAADIKMIITMAEYAERVEEIREECPTLAVRLMIDGTRDGWVSYPVELDYPAPCSH
                     KLVNLPGMRRTRSSDPLLIFFTSGTTGEPKMVVHDHAYPLGHLVTGQVWHDLHPNDLH
                     LTISDTGWGKSAWGKLYGQWIIGACIFVYDIRSRFHATEILPLLERYGITTFCCPPTI
                     YRMLILADLDKFDLADMRHCCSAGEPLNPEVIRAWQEGTGRTIYEGYGQTETVLCIGT
                     FPGMKCKPGSMGRPAPGWHIELHDDDGNPVGVGEEGRIAIKLDPRPVGLFRGYLNNEE
                     ENCKVFQNGFYYTGDKACMDEDGYFWFIGRDDDVIKSSGYRIGPFEVESALMEHPAVQ
                     EAAVIGSPDVIRGLVVKAFITLKPGYNPSESLIKDIQKHVKRVTAPYKYPRAIEFVES
                     LPKTISGKIKRYELREREMKQFMDNNSHGVHSGSKCAE"
BASE COUNT         1365 a         1978 c         2150 g         1346 t
ORIGIN      
        1 gttcgatata gccgagacga cctcgaagat gacatcggtg gagtcaaacc ccccctgcgg
       61 cacaaggtgg agcaggagaa gggtggcgac cgcgaccgcc aggatgaaga gcacgacgac
      121 gagcatgctc tttgagacct cgtactccgg gagatgttcg aggatcggcc ggccctcgtg
      181 ccggagcggg gtgatcgtcc gccaccctgc gcggagcctc cggatccacc acccgagcgt
      241 ctcaaggccg aggatgaccc tgccgagttt gatgccgccg gcggtacttc ccgccgaccc
      301 gcctatcagc atcagcgcgg tgagcaccat cacggttgcg ccataccagt cacgggggtt
      361 tgcgttctgg aacccggtgc aggtgatacc gctcaccgtc atgaagatgc cctcccggag
      421 ggcctcggcg ggggcgaggg aagagagggc cacgagatcg aaggtgatgg cggtgcatcc
      481 gacaaggatg agtgcaaaga ggaggattgc ctgcgaatcc cggaaaaatt cgaacgttct
      541 ccggtagtag aggataaaat agagtttgaa cggcagagca ccggcgatca tgaccgggac
      601 gacgagcatc tcaaggagcg ggctctcgta gtagaggatg ccagccgagt gcactgcaaa
      661 tccgccggtc gatatcgccg tcatgacgat gttcacggcg tcccagaggg atatgcccga
      721 gaggagaacg aggccgatgc cgagggccgt gagcaccagg tatatccgcc acatctccgt
      781 agcggtggcc acgacgcccg gcatgaacgc ctccgaccgc ccctccgagc ggtagagctg
      841 gaatcgggtc aggcctgacc tgcccgcgag agcgatggtg aacgcaacga tgccgagccc
      901 gcccatccac tgggtcagcg accgccagaa gaggatcatc cgtggaacgg tatcgaccga
      961 cgggagaagg gagaaccccg ttcccgtcca gcccgacatc gcctcaaaga cgctgtcggt
     1021 ataggggatt ccaaggccga acatgtaggg gagcgcgcct atcagggcgg cagcaagcca
     1081 gatcagggcg accgcagaga gtgcgacgga gaggtgcgct tctccctccg ccttcgcgac
     1141 gctggcaagc cagaccccga ggatgccgaa ccccgccggc gccgaggcca tcgggaggag
     1201 gaggtcgagc tcgccatacc agaaaccgac aaggaacggt gtgcaggaga ccagcgcgat
     1261 gaagaggaag atgagcccga ggtcggaagc gatgatcgca aactggctgt ttgccgccgg
     1321 cagtaccttt ttgccggaga acgccgggaa gaaacggtct ctacgacggt ccattgccgc
     1381 acctgatgcg gttttacggg atcaccagct gaattatgga catggaaaac ccggtatatg
     1441 tagtttttgg cgacctgtcc ggctccgcac acggcgatgg cgagggccgt acgaccggag
     1501 agatgacttc ggcggagagg gcggccgtga cgatggggtt tgaggagccc gatcgtgtcc
     1561 cgctcttcac catatacggg gcgaaagacc tcgggctctc catccgggag tactcctcaa
     1621 aactgaagta tgtcgccgcg ggccagatgc gtctcctgga gaagttcggc ggcatagact
     1681 gccccgccca tcagggcggg gtttgcagat agcagccctg cgatcttatg gaactatctc
     1741 ttctccaagg gggccgagtg ccgggttcgc cgcggggatg gtcggtgccg tcaggatatc
     1801 cctgataccg gcagagataa tagtatcccc actgcataga ccatcatgcc cctcccttcc
     1861 tgcacccttg cgcaggggcg gtatacggtg tgccgaccgc tgccggaagg ccggtcttca
     1921 caaccccttc cgacagtgag aggaagcgac ggggtgggta gccggagggg caccaccggg
     1981 ttgatgcggg gcgttcctgg cttcccgcct cgtgccgggg attggaggtt cattgcatga
     2041 ctctcgtgat ggcatacatc ggaagacagg gcgccgtgat ggcgggcgat atgcgggaga
     2101 tcgcattcca gggagacgac ccctgtatag aagcgctgga gcgcgagctc tacagcggtt
     2161 cgatcgcgtc cgacaacgac ctgctggagc gggccggtga gatcggggtg gcaatccggg
     2221 tcagggatga taaggtcaaa gtcacggagc aggacggggt gctcgtcggc gaggtgaccg
     2281 agaccgcggg atcgacgatc cgccaaaaga ggctgtacgt cactgcaggg agctacgtca
     2341 tcgccgagat catcgactcc cggttacgcg tgacacagag aggccaggcg ggcaatttcg
     2401 tggtgctggg caacgatata acgaagcagg tcgccggcca gtgtatccgc gaggcgtgga
     2461 agggtgggac ggttcctgag gccatgcggg tcatcctgct caccatgcag atggcgtcag
     2521 gcgtaacggc gtcggtgagc agaacgttta tgctggtgca taccgatcgt gcgatgaacc
     2581 ttgccggtgc gataaaccgg gcttccgggg gagaataggt ttcgggaggt gttcgattcc
     2641 gccggtggcc ggtgaaggtg tcccggccgt agcagagacg gcgctactgg ctgaggtagt
     2701 tgtgcttctt gagccagtca acctggggcc tgatgtaacg gtagatgatg tcgcactttt
     2761 catcctggaa actccaggga acaacaatca ggaggtcgcc ctcgcgtatc cagagccgct
     2821 tcttgatctt gcccttgatc cgccccatgc gcgtgacacc gtcctcgcaa cggacgcgga
     2881 tatggtttgc cccgagcatc aggtcggccc ggccaaataa ctcccggttt cgttttttcg
     2941 gtaatcgtac ccgtacaatc tcttcgcttc cgctgttctg tgatcctcgt gtatttgtca
     3001 gttcatcaac tccgtgacag gtagcgataa gctgcctatc ggttctttcc tggaggtgta
     3061 tactaatgac cccggccacc attaatgtat ctattccagg aaaattcctt caatccgggt
     3121 ccgccgggga agtgcaggca tgacgagggt ggttatcata tcccatgtct cccggtcgcc
     3181 cgaaccgttc cgccggggcc tgcggtgagg tggtgagagc actcccgtca cctgatcgac
     3241 tgcatggtgg gcggtgccgc gcattcacat gggtctgccc ccccgggata caccggcggc
     3301 ggtacgggga gatacgcccg gggttttaca tgatatctcc gtccgggcgg agcggctacc
     3361 gtgccggggc gcatacaccc gggcacgatc ccccgcccct ctcttgtgtt ctgggcaaac
     3421 gccgcccggg aatgcacacc tcctctgcca tgtgcctcct ctacccggga aaatcgaccc
     3481 cgggcgggca cccgtgggtt tatccgaaga gtagttaagg tttgagcgta catgtgtgaa
     3541 gaggatcttt gaatggaaag cagcaagctg tacgtcggaa acctcaccta ctcggtaaat
     3601 gaagagcagt tgaaagaatt gtttggccgg tacggaacgg tgaacgacgt cagagtcatt
     3661 gagcgcaaag ggttcgggtt cgttgagatg tccagcccgg aagaggctga gaaggcaaaa
     3721 gacgccctga acaacaccgt attcgagggc cgcaccctga agatcgacga ggcccggcct
     3781 ccgagaccca gaagcgactt ccggcggtac tgaaccccgc ccggaagggt agtactctga
     3841 atatcccctt ttttttatga atcggggttc gcaagaacct catctctgat tgaccggcgc
     3901 gcatgcccct ccgcccgggc actgctgtcg ttcctgcccg gagccgttct cctgaggtag
     3961 ccagaaccgg ggctgacttg ccggtgggaa gtcccgacca cccggcagct acctgctata
     4021 taggtccggc cgggtatatc gggggtggtg atgcataatc gggtccaata tacccggggc
     4081 gaaaaatccc ctggacgctc tccatggtga gcacgccagc catccgctga gggtggttat
     4141 agggagagac gatgcctata attattacat cgattccacg cagaaagcgc tctatgcctg
     4201 tgtcctttcc ctcagaaaag actttacaat ccttgatttc gggtgcggga acggccggtg
     4261 ggcattctgg ttcgcaccac gggtggcccg cgtcatcgga atcgacctct cgcctgcaat
     4321 ggtggatgca gcccggcggt tcgcgtcgga gcagggtatc cagaacgtcg gtttcaccgt
     4381 tcccggtgac gggtcacagt ttcctgacga ttccttcgat atcgtgaact gtcggccgcg
     4441 acctcatctg caccgggcct gcgatgggta actcgggtgt ggacccatat ccatacgttc
     4501 atctcccgga tccccctctc accctccgac accgcgcgcg gcccgccttt cctcccggca
     4561 cccgcacggg ccgatcaccc tccctgactg aaaccaatcc tctgccgtat catcgatttt
     4621 tcagcctaag aacacagcaa catttttatt caccaagagc tatggggcag gcgtgtgtcc
     4681 cgctgtgcgg gatgcagtca acaaggtggt gagaagtatg gcagaagaga ggagaagcgt
     4741 gatcgaggag gagagaccat cggtcagggg tggacatgcc tctgcggccc aggccggatc
     4801 ggtcgaggag ctgagcgcgt ctgcgttcca gtcgtacctt cacggcatgg actaccccgg
     4861 caagaaacag gacctgatca acctcgcccg gaagaacaac gcgccggatg cggtcatcca
     4921 ggttctcgag atgttcaacg atgacaagac cttccagtcc gcagccgatg tgagccagga
     4981 gtttggcagg gtcaaataac cttacccatt tttgaacata tcaaagcgcc ttccgacctc
     5041 gttagccgga cagcaccgat aaaatcggga gaaggatgcg agaggccgga ttcgaaccgg
     5101 cgaactccta cgagaatagg ccctgaacct atcgcctttg acctggcttg gcaactctcg
     5161 cgcctagtat tgtaatacct tcaacaaaat aaagatgttt actccgcgca cttcgagccg
     5221 gagtggacac cgtgggagtt gttgtccatg aactgtttca tctccctctc gcggagttca
     5281 tagcgcttga tcttgccgga gatcgtcttt gggagcgatt ccacgaactc gatcgcgcgc
     5341 ggatacttgt agggtgccgt cacccgtttg acatgcttct ggatatcctt gatgagggac
     5401 tccgaggggt tatacccggg cttgagagtg ataaacgcct tgacgaccag gccccggatc
     5461 acatccggcg atccgatgac cgccgcctcc tggacggccg ggtgctccat aagcgcgctc
     5521 tccacctcga acggcccgat ccggtagccg gatgacttga tgacgtcgtc gtcgcggccg
     5581 atgaaccaga agtagccgtc ctcatccatg catgccttgt cgccggtgta gtagaacccg
     5641 ttctggaaga ctttgcagtt ctcctcttcg ttgttcaggt agccgcggaa gagcccgacc
     5701 ggacgcgggt ccagcttgat cgcgatgcgc ccttcctccc ccacgccgac cgggttcccg
     5761 tcgtcgtcgt ggagctcgat atgccagccc ggcgccggcc tccccatgga gccgggtttg
     5821 cacttcatgc cggggaacgt cccgatgcag agcactgtct cggtctggcc gtagccctcg
     5881 tagatcgtcc ggcctgtccc ttcctgccac gcccggatga cctcggggtt gaggggttcg
     5941 ccggcgctgc agcagtggcg catatcggcg agatcgaact tatcgaggtc ggcgaggatc
     6001 agcatccggt agatggtcgg agggcagcag aaggtcgtga taccgtatcg ttccagcagc
     6061 ggcaggatct cggtggcgtg gaaccggctg cggatgtcgt agacgaatat gcacgcgccg
     6121 atgatccact ggccgtagag tttcccccac gcgctcttgc cccagccggt atcggagatc
     6181 gtcaggtgga gatcgtttgg gtgcaggtcg tgccacacct gcccggtgac gaggtgcccc
     6241 aggggatagg cgtgatcgtg gaccaccatc ttgggttcgc cggtggtgcc ggaggtgaag
     6301 aagatcagca gcgggtctga ggaccgggtc cggcgcatgc cggggaggtt caccagtttg
     6361 tgcgagcagg gcgcggggta gtcgagctcg acggggtaac tgacccatcc gtccctggtg
     6421 ccgtcgatca tgagacggac cgcaagcgtg gggcactctt cgcggatctc ctcgaccctc
     6481 tccgcatact cggccatggt gatgatcatc ttgatgtcgg ccgcctggat gcggtatttc
     6541 agatcttttg ccgtcagcat cgtcgttgca gggcagtata ccgcgccgat cttgatgagt
     6601 gcgatggtga agatccacca ttcgggggcg cgggggagca tcagcatgac ccggtcgccc
     6661 ttcttgatgc cgtattttat gcagatgttg actgcctgat tggagaggcg catgaggtcg
     6721 aagaatgtgt atttcttctc gtttcctttc tggtcggtcc agatcatcgc cagcttgttc
     6781 cgatccttct ttgcccatgc gtcgatcacg tcaaagccga agttatagta ttcagggac
//
LOCUS       DAOSEE010000116         6596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_4217_length_6596_cov_219.632625, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000116 DAOSEE010000000
VERSION     DAOSEE010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6596
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4217_length_6596_cov_219.632625"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..116)
                     /locus_tag="PLI76_08675"
     CDS             complement(<1..116)
                     /locus_tag="PLI76_08675"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HOI62146.1"
                     /translation="MKKRPPMNRAERAQMAMMLEVSAYPKPGNVDRCHDYADT"
     gene            complement(85..1224)
                     /locus_tag="PLI76_08680"
     CDS             complement(85..1224)
                     /locus_tag="PLI76_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 9 domain-containing
                     protein"
                     /protein_id="HOI62147.1"
                     /translation="MMEAYDRFELLINDRIVKTPVAIASMAGVVDAGYVLERAAHVGA
                     AFIGGYSIDGPTLDASRRMAAEGRREFLYDDPLDALAKEIDALKGSDVITGVNLRGST
                     PAAYVDVARAFEDRVVYEIDAHCRQPAMLEAGCGEYLLQHPSKLTEIIRALKSANVTV
                     SVKIRAGVAADDRDLARAIWKAGADILHADLMDYGYARVRQIRNASPLMLIANNSLNT
                     FEKAMDAFSHGADLVSLARRSDPWTLAGLDAAITRYADEGGWYNAPKQLCRGGDIRAL
                     TFCCMPVKACPLIPTLEKIGLSRNEYLKLKQEAVKGTPLDDGKGTCFGGLAWCCKISS
                     PCMFRNMTLEQKGLSAQEYMRCKHRLSEKILHRLFDEEETADEPS"
     gene            1489..1896
                     /gene="cfbA"
                     /locus_tag="PLI76_08685"
     CDS             1489..1896
                     /gene="cfbA"
                     /locus_tag="PLI76_08685"
                     /EC_number="4.99.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin nickelochelatase"
                     /protein_id="HOI62148.1"
                     /translation="MSMKGMLLVGHGSKLPYNKELIETTAAIIAGKTDEYLVKPGFMS
                     INAPTVEEQLAAFRREDIDMLVVVPLFLARGIHIEQDIPRLLGLAPGVRTGEFQLNGK
                     TVPLVYANPIGSDPLLAELMLKNASDAIAELKP"
     gene            1901..3079
                     /gene="cfbE"
                     /locus_tag="PLI76_08690"
     CDS             1901..3079
                     /gene="cfbE"
                     /locus_tag="PLI76_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F430 synthase"
                     /protein_id="HOI62149.1"
                     /translation="MRILVLDTIHGGAELAVALREAGHEADEVDVYRGKAGIPVAEAL
                     ARTYDLVTAPVHLDPDHPLLRQHGYAVSHHEMVGWVLRAHPPRPFIEITGARGKTTTA
                     HALASLLPGPGILHTSTGTYRYPERKILWKKSITPASLLSAAHEAWRTGGWLVAEESL
                     GVTGAGDVAVLTSTDDYPIAAGKKSAIAEKCRLITRARTIVVPERIDLPRSAVAADGM
                     VSFEGPVCRYAGRGIAGTFTNPLCTLSGYRTAFALATATACVLGIDPAPLAEFTALSG
                     RMAARREGDRMIVDDANSGTNAATVIEAARYARELTGNGALTLVIGEEARAVCEGFSP
                     EDVRRAVSAVSPTGVVYVGEAWSAAGEEHTALSLEAGLDAARRITPAGAIVLAVKTWR
                     "
     gene            3082..4146
                     /gene="cfbD"
                     /locus_tag="PLI76_08695"
     CDS             3082..4146
                     /gene="cfbD"
                     /locus_tag="PLI76_08695"
                     /EC_number="6.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ni-sirohydrochlorin a,c-diamide reductive
                     cyclase catalytic subunit"
                     /protein_id="HOI62150.1"
                     /translation="MDYIQPRPSSIVAALYTARDLGVDVAILHGPSGCSFKHARLLEE
                     DGMRVLTTSLADNEFIFGGHDPLVRVLRHAEKEFTPRRMAVVGTCVAMIIGEDLQSAL
                     HDAGTRVPAIAVDIHAGFRENIDGVLATLEPAAAAGWISGAELERQRYLLGKANEVER
                     LRGAASRHYIEPSRGDLKHVAARRLVDLADSGAIGVAVMNAKKETAYMFADELVALHD
                     TCPDAAITYVANLEDRGLPKVRNDAARILAGMRERGVDPELLGALDEYGANGDAIGER
                     LREMKPDFALMVGVPHAVPPEYTEGIEVFSITNGPRQVEPLRDMGHRHVMVEIDLHPK
                     TLGVREIVASEFGAVLRSMR"
     gene            4143..5510
                     /gene="cfbB"
                     /locus_tag="PLI76_08700"
     CDS             4143..5510
                     /gene="cfbB"
                     /locus_tag="PLI76_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni-sirohydrochlorin a,c-diamide synthase"
                     /protein_id="HOI62151.1"
                     /translation="MKSFLVAGDRSGSGKTSITLALSALLSATRAVQTFKVGMDYIDP
                     SYLTGVTGRPCRNLDGYVMSPAEVRAVFAHGCRGADVAVVEGVRGLFEGAEALTDLGS
                     TAAIAKQLDLPVVLVVNARSITRSAAAIVKGFQAFDPDVAIRGVILNQVTGARHREKA
                     VRAIEHYCGIPVVGVIPRTADMELAMRHLGLVPYREGQEHGEFLARIEAVKQMIGEHV
                     DIDALLSLAKESVLPAGEDPIYAAVDPDVRIGVALDEAFNFYYADLFDVLASSGAEVV
                     PFSPVHGRLPEADGYIIGGGSPEVFGPELEANTAVREGLREASRNGIPVYAECGGLVY
                     LTERMVLAPGFTASKREETYDLAGVFAGEARMPEKRMLGYVVGTSAGENPMGAAAFKG
                     HEFHYSSVRLAPETRYAYRLTRGGGIRDGLDGAVRDRTIGSYTHLHPVTSRGMFANFV
                     AGCRG"
     gene            5602..6471
                     /gene="ilvE"
                     /locus_tag="PLI76_08705"
     CDS             5602..6471
                     /gene="ilvE"
                     /locus_tag="PLI76_08705"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HOI62152.1"
                     /translation="MIIYLDGRYVPEEEAKVSVFDHGLLYGDGVFEGIRAYNGRVFRL
                     DEHLARLYDSAKTIDLAVPITREEMAEAIKETLRKNNLKDAYIRPIVTRGKGDLGLDP
                     RKCARPTVIVIAVSWGAMYGDLYEKGLRAVCVSVRRTPPEAMPPNVKSLNYLNNILAK
                     IEANYRGVDEAIFFDTNGYISEGSGDNIFVVKNGAIITPPTLNNLRGITRTVALEIAA
                     SMGITLLERNLGYYDLYTADEVFVTGTAAEVAPIREIDGRVIGNGKPGPVTRQLMAAF
                     KTVTQKEGAPVYK"
BASE COUNT         1167 a         2108 c         2216 g         1105 t
ORIGIN      
        1 gtgtcggcgt agtcgtggca ccggtccacg ttcccgggtt tcgggtaggc cgagacctcg
       61 agcatcatgg ccatctgcgc ccgttcagct cggttcatcg gcggtctctt cttcatcgaa
      121 cagcctgtgc aggatcttct cggacaggcg gtgcttgcac cgcatgtact cctgcgccga
      181 gaggcctttc tgctcaagcg tcatgttccg gaacatgcag ggggaactga tcttgcagca
      241 ccaggcaagg cccccaaaac aggtgccctt cccgtcgtcg agcggggtgc ccttcaccgc
      301 ctcctgcttg agcttgaggt actcgttccg ggagagcccg atcttctcga gggtcggtat
      361 gagggggcag gccttcaccg gcatgcagca gaaggtgagc gccctgatgt cgccgccgcg
      421 gcagagctgt ttcggggcgt tgtaccagcc gccttcgtcg gcataccggg tgatggcggc
      481 atcgaggccg gcaagcgtcc aggggtcgga gcgccgggca agcgagacga ggtccgcccc
      541 gtgagagaag gcatccatcg ccttctcgaa ggtgttgagg gagttgttcg cgatcagcat
      601 caggggggag gcgttgcgga tctggcggac cctggcgtag ccgtagtcca tcaggtccgc
      661 gtggagtatg tccgctcccg ccttccagat agcgcgggcg aggtcgcggt cgtcggcggc
      721 gacgccggcc cggatcttga ccgatacggt cacgttcgcc gacttcaagg cgcggatgat
      781 ctcggtgagt ttcgaggggt gttggagaag gtattcgccg cacccggcct caagcatcgc
      841 cggctgccgg cagtgagcat cgatctcgta gaccacgcgg tcctcaaacg cgcgggcgac
      901 atcgacgtac gcggccggcg tgctcccgcg gaggttgacg cccgtgatga cgtcgcttcc
      961 cttcagggca tcgatctctt tagccagggc gtccaggggg tcgtcgtaca ggaactctct
     1021 tcggccctcc gctgccatcc gccggcttgc atcaagggtg gggccgtcga tggaatagcc
     1081 gccgatgaag gcggccccca cgtgagccgc cctctcaaga acgtaacccg catccactac
     1141 tccggccatg gatgctattg ccaccggtgt ttttacgatc cgatcgttta tgaggagctc
     1201 aaaacgatca taagcttcca tcatgcttct ctattcgttg tcggtatgag gggatattga
     1261 tttgtatcgg gtcagggacc ggtcggcacc cggcgcccgg gaggcccgta taccgggcgc
     1321 gctccggccc gggcgataac agggccttcc ggcgttccgg cgtacaaaaa gccggcagat
     1381 atcgggggcg acgatccatc cgaagcccgg catcgccgcg ataccgggcc ggacccttac
     1441 gaacaatcag taattatttt aatatagtat cgtagtaccc tgtaacatat gagtatgaag
     1501 ggaatgttac tggtcgggca tggcagcaag ctcccctaca acaaggaact gatcgagacc
     1561 accgcggcga tcatcgccgg gaagaccgac gagtatctcg tcaaacccgg tttcatgagc
     1621 atcaacgcgc cgacggtcga ggagcagctt gcggcgttcc ggagagagga tatcgatatg
     1681 ctggtcgtcg tcccgctctt cctcgcacga ggcatccaca tcgagcagga tatacccagg
     1741 ctcctcggtc ttgctcccgg ggtaagaaca ggcgaattcc agctgaacgg gaagacggtg
     1801 ccgctcgtct acgcaaatcc catcgggagc gacccgctcc ttgccgagct gatgctgaag
     1861 aacgcatccg atgcgatcgc cgaactgaag ccctgaggct atgcggatcc tggtgctgga
     1921 taccatccac ggcggggcag aacttgccgt ggcgctccgg gaggccggcc acgaggcgga
     1981 tgaggtggat gtctaccgcg gaaaggccgg cattcctgta gcggaggcgc tcgcccgcac
     2041 ctacgacctc gtcaccgcac cggtccacct cgacccggac catcctctcc tccggcagca
     2101 cgggtacgcg gtctcccacc acgagatggt ggggtgggtc ctcagggccc accctccccg
     2161 cccgttcatc gagatcaccg gtgcacgggg caagaccacc acggcgcacg cgctcgcttc
     2221 gctgctgccg ggcccgggca tcctgcacac gtcgaccggg acctaccgct atccggaacg
     2281 aaagatcctc tggaagaaga gcatcacgcc cgcctccctg ctgtcggcgg cgcacgaggc
     2341 ctggcgcacc ggaggctggc tggtcgcgga ggagtcgctc ggcgtcacgg gggccgggga
     2401 tgtggcggtg ctgacatcaa cggacgatta cccgatcgcg gcggggaaga agagcgcgat
     2461 cgccgagaaa tgccggctga tcacccgggc gcggacgatc gtggtcccgg agaggatcga
     2521 cctcccgcgg tcggcggtcg ccgccgacgg gatggtctcc ttcgagggtc cggtctgccg
     2581 gtatgccggg cgcgggattg ccggcacttt cacaaacccg ctctgcaccc tctccggcta
     2641 ccggacggcg tttgcgcttg caaccgcgac ggcctgcgtg cttggcatcg atcccgcccc
     2701 tctcgcggag tttaccgctc tttccggccg tatggcggcc cggcgagaag gggatcgtat
     2761 gatcgtggac gacgccaaca gcggaacgaa tgcggccacc gttattgaag ccgcccgtta
     2821 cgcgcgggaa ctcaccggga acggcgccct gacgctcgtc atcggcgagg aggcgcgggc
     2881 cgtctgcgag gggttctccc cggaggatgt ccggcgggcg gtatcggccg tcagcccgac
     2941 gggagtcgtt tacgtcgggg aggcgtggag tgcggccgga gaggagcaca cggcactctc
     3001 gctcgaagcg gggctcgatg ccgcccggcg gatcacccct gcgggcgcga tcgtccttgc
     3061 ggtaaagacg tggagatgac catggattac attcaaccaa gaccgagttc cattgtagcg
     3121 gcgctctata ccgcccgcga cctgggggtg gacgtggcga tcctccacgg cccgtcaggc
     3181 tgttcgttca aacacgcccg tcttctcgag gaggacggca tgcgggtgct gacgacgtcg
     3241 cttgccgaca acgagttcat cttcggcgga cacgaccccc tcgtgcgggt cctccgccac
     3301 gcggagaaag agtttacgcc ccggcggatg gcggtcgtcg ggacgtgtgt cgcgatgatc
     3361 ataggagaag atctccagtc cgcgctccac gacgccggga cccgggtgcc cgctatcgcc
     3421 gtggatatcc acgccgggtt ccgcgaaaac atcgacggag tgctcgcgac gcttgaaccg
     3481 gcggcggctg ccggctggat cagcggcgcc gagctggagc ggcagcgcta cctcctcggc
     3541 aaggcaaacg aggtggagag gctccggggg gcggcgtccc ggcactacat cgagccctcc
     3601 cgcggcgacc tcaagcacgt cgcggcacgg cgcctcgtgg acctcgccga ctccggcgcc
     3661 ataggggtcg cggtcatgaa cgcgaagaaa gagaccgcgt atatgttcgc cgacgagctc
     3721 gtcgccctcc acgacacctg tccggacgcc gcgatcacct acgttgcgaa cctcgaagac
     3781 cggggcctcc cgaaggtccg caatgacgcc gcacggatcc tcgccggcat gcgggagcgg
     3841 ggagtcgacc ccgaactcct cggggcactc gacgagtacg gcgcgaacgg ggatgcgatc
     3901 ggggagcggc ttcgcgagat gaaacccgac ttcgccctca tggtcggcgt gccgcacgcc
     3961 gtcccgccgg agtacacgga agggatcgag gtcttctcga tcacgaacgg gccgcggcag
     4021 gtggagccgc tccgcgacat ggggcaccgg cacgtcatgg tcgagatcga cctgcaccca
     4081 aagaccctcg gcgtccgcga gatcgtcgca agcgagttcg gggcggtcct ccggagcatg
     4141 cgatgaagtc cttcctcgtc gccggcgacc gctccgggag cgggaagacg agcatcaccc
     4201 ttgcgctctc tgccctcctc tcggcaacgc gggccgtcca gacattcaag gtggggatgg
     4261 actacatcga cccgtcctac ctcaccggcg tgacgggccg gccctgccgg aacctggacg
     4321 gctacgtgat gagcccggcc gaggtccggg cggtctttgc gcacgggtgc cggggagcgg
     4381 acgtcgccgt cgtcgaaggg gtcagggggc tcttcgaggg cgcggaggcg ctcaccgacc
     4441 tcgggagcac ggccgcgata gcgaaacaac tcgacctgcc ggtcgtcctg gtcgtcaacg
     4501 cccggagcat cacccggagc gcggcggcta tcgtgaaagg tttccaggct ttcgacccgg
     4561 acgtcgccat ccggggggtc atcctgaacc aggtgaccgg cgcccgccac agggagaagg
     4621 cggtcagggc gatcgagcac tactgcggca tccccgtcgt cggggtcatc ccccggacgg
     4681 cggatatgga acttgccatg cgccatctcg gcctcgtccc ctaccgggaa ggccaggagc
     4741 acggggagtt cctcgcacgt atcgaggcgg tgaagcagat gatcggcgaa cacgtcgata
     4801 tcgatgcgct gctctcgctc gcaaaagagt ccgttttacc ggccggagag gaccccatct
     4861 acgcggcagt cgatccggac gtccggatcg gcgtcgccct cgatgaggcg ttcaacttct
     4921 actacgccga tctcttcgac gtgctcgcgt cctctggggc agaggtggtt ccgttctccc
     4981 cggtccatgg ccggctgccg gaggccgacg gctacatcat cggcggcgga tccccggagg
     5041 tctttgggcc ggagctggag gcgaacaccg cggtgcggga ggggctcagg gaggcttcac
     5101 gaaacggcat cccggtctac gcggagtgcg gggggctcgt ctacctcacg gagaggatgg
     5161 tgctcgcccc cggctttaca gcctcgaaga gggaggagac ctacgacctt gccggggtct
     5221 ttgccggcga ggcccggatg ccggagaagc ggatgctcgg ctacgtcgtg gggacgagcg
     5281 cgggcgagaa ccccatgggg gcggcggcgt tcaagggtca cgagttccac tacagcagcg
     5341 tccgcctcgc gccggagacg cgctacgcct accggctgac ccgggggggc gggatccgcg
     5401 acgggcttga cggtgcggtc cgggaccgga cgatcgggag ctacacgcac ctccacccgg
     5461 tgacgagccg ggggatgttt gcaaacttcg tggcgggctg ccggggctga gaggaggcgc
     5521 cggcattccc ggggggagag gtcgtgccgc cgggatcatg aagcgcaaga catacaaacg
     5581 ccccggtctc atactgttct tatgattatc taccttgacg gcagatacgt cccggaggag
     5641 gaggcgaagg tctcggtctt cgaccacggc ctcctctacg gcgacggcgt tttcgagggg
     5701 atccgcgcct acaacggaag ggtcttccgt ctcgacgagc acctcgcacg gctctacgac
     5761 tcggccaaga cgatcgatct tgccgtcccg atcacgaggg aggagatggc cgaggcgatc
     5821 aaggagacgc tgcggaagaa caacttaaag gacgcctaca tccgtccgat cgtgacccgg
     5881 gggaagggcg acctcggcct cgatccccgg aagtgcgcga gaccgacggt catcgtcatc
     5941 gccgtctcat ggggcgcgat gtacggcgac ctctacgaga aggggctccg ggcggtctgc
     6001 gtctcggtca ggcgcacccc gccggaggct atgccgccga acgtgaagag cctcaactac
     6061 ctcaacaaca tcctcgcgaa gatcgaggcg aactaccgcg gcgtggacga ggcgatcttc
     6121 ttcgacacca acggctacat atccgagggc tcgggggaca acatattcgt cgtcaagaac
     6181 ggggccatca tcactccgcc gacgctgaac aacctgcgcg gtatcacgcg gacagttgcg
     6241 ctcgagatcg cggcatcgat ggggatcacc ctgctcgagc ggaacctggg ctattacgac
     6301 ctctatacgg cggacgaggt cttcgtcacc gggaccgcgg cggaggtcgc gcccatccgc
     6361 gagatcgacg ggcgcgtcat cgggaacggc aagcccggcc cggtcacccg gcagctgatg
     6421 gcggccttca agacggtcac ccagaaggaa ggggcgccgg tttacaagtg aggagagggg
     6481 ccattcgccc cggattctcc ggcacaactt ttttatcttc tggcatcgaa acgatatggc
     6541 aacactgtgc atgctgctat agtgtagagg ccaatcatgt cggcctttca cgccga
//
LOCUS       DAOSEE010000117         6542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_4259_length_6542_cov_250.312471, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000117 DAOSEE010000000
VERSION     DAOSEE010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6542
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4259_length_6542_cov_250.312471"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PLI76_08710"
     CDS             complement(<1..182)
                     /locus_tag="PLI76_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase type 11"
                     /protein_id="HOI62153.1"
                     /translation="MLRRVGNQGALLAELRRITKPGSALTPNDGHQPREKTRRAIHDA
                     GIWRVEEENREYLRCRP"
     gene            complement(766..1785)
                     /locus_tag="PLI76_08715"
     CDS             complement(766..1785)
                     /locus_tag="PLI76_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HOI62154.1"
                     /translation="METKTFKNLEAEVWTTDLCSGCGACVAVCPADALRFVPGKTDAP
                     VNIGYCKSENDGVPCGACYAACPRVDLAGQGRMLGAYREIVAARSAFPVENKQSGGAV
                     TAILANALDEGLIDAVVTVTRDPWTLKPSSAVITSSDALIHHAGSRYSWWVPLLASLK
                     EAVVTRKYRRIAVVGVPCVARATQAIRASDHDLLRPYAKAIRLVIGLFCTETFDYAKL
                     VEEKLQSEKKIEPWEIQRLDVKGKLDVYLQDGRHIPIPLADLEECVRPGCRVCTDLTA
                     VDADISAGAIGSPDGYTTLVIRNDIGRGFVDRAVWKGKLATGGNVDIAAIERLAAKKA
                     ERRKE"
     gene            complement(1791..2528)
                     /locus_tag="PLI76_08720"
     CDS             complement(1791..2528)
                     /locus_tag="PLI76_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62155.1"
                     /translation="MTKLVTIDAKGVHYTPLNQQIRKAIEDGADEIVVDNVLGQRFIG
                     NGLRGNAVIRVNGVAGGDLAMFMSGPTVVVHGNAEHAPGNTMDSGKVVIHGSAGDAVA
                     HSMRGGKVFVRGDIGYRGGIHMKQYEAHRPILVVGGSAQAFLGEYMAGGLLLVLALDG
                     AMKAREIGSGIHGGEIVIRGNVDEACLAAGAKTLPLTDEDRARIAPVIREFADDFGLD
                     PEPLVNADYTRIVPASARPFAGKYTWE"
     gene            complement(2534..4051)
                     /locus_tag="PLI76_08725"
     CDS             complement(2534..4051)
                     /locus_tag="PLI76_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HOI62156.1"
                     /translation="MSSNIGSMPLRYRVTIDRERCMECGRCIENCSYGVFRWDGDRIL
                     INSRNCTACHRCLAYCPRDAIMLEEHPCDYRSHPVWSRQVRESIYNQARTGKIILAGM
                     GSVADLPIIFDHLMLDACQVTTPPTDPLREPVELRTYLGKKPSRLDFARKPNGDIEVS
                     TELAPNLMLETPIMLGHMSYGAISLNAHLAMARAAKETGTYMGTGEGGLHPGLYPYQD
                     RMIVQVASGRFGVNVDYLERGAAIELKLGQGAKPGIGGHLPGEKVSADVSRTRMIPEG
                     SDAISPAPHHDVYGIEDLPQLVNSVKEATGWKKPVFAKIAAVNNNAENVAAIARSGVD
                     AVAIDGFRGGTGAAPRVFRDHVGIPIEVAIATADRELRKQGLRNEVSLIACGSIRESA
                     DVVKAIALGADAVYIGTAALVAMGCRVCGNCYQGVCSWGIATQRPNLVARLDPDVASK
                     QVANLIHAWTLEITELMGAAGINSIESLRGNRDRLRGYMLDEGLLEILDVKSVGA"
     gene            complement(4048..5109)
                     /locus_tag="PLI76_08730"
     CDS             complement(4048..5109)
                     /locus_tag="PLI76_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62157.1"
                     /translation="MCGIFGVMDRRRQMMDGSGIRQALSIMNERGSGEGAGYVAYGIY
                     PDYRDCYALHVFLDHACESKAVVDATLEQWGAIEHDEAIPTCDQPNFRAACTPWRYFF
                     KPDPSLAPGSATPEKDIITNLVMQINASPDGTEVISSGKNVGVFKAGGAGSWPETVAD
                     YYRIEDYEGYIWLAHNRYATSCPERWERPDPFNLHDWSIVENGKTTTYGANRRYIESF
                     GYNCSTGTDSEVIAYTIDFLVRRHGLEIDLAIRALAPPYWDEIDRMPEAEQKLNRALR
                     FAYGPATMNGPFTVVAANPDMMVGFTDRGKLRPMVVGECGDRLYIASEETAIRTMEPG
                     VESITRPGAGEPVIGRVAP"
     gene            5213..6541
                     /locus_tag="PLI76_08735"
     CDS             5213..6541
                     /locus_tag="PLI76_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HOI62158.1"
                     /translation="MSRDATSAMLERIGEDNVRFLHLQFTDLLGMPKNVAIPVKQAEK
                     ALTGGIGFDGSSIQGFVRIEESDMILKPDLSTYTLLPWRSGEYTVARFMCDVYKANGK
                     PFEGDPRYVLRTAMEDAARDGYIFNTGPELEFFLFRTIDGKPTTFFQDAGGYFDLAPT
                     DLAEDVRREIIIALTEMGFEIEASHHEVAESQHEIDFKYSDALHTADNVITFKFVAKT
                     MALMRGLHASFMAKPIYGICGSGMHTNCSLAKGGVNAFYDPDAPRELSTACMHFIGGL
                     LRHAKAITRVANPTINSYKRLVPGYEAPCYISWSAGNRSALVRVPTPRGNSTRVEFRS
                     PDPTCNPYLTFAAMLTAGMEGVREKIEPPAGVDKNIYHMAAEDRRCAGIETLPGDLYS
                     AHQALLADDLICRALGPHVVEALTNVAEAEWEAYRTAVHPWELERYLATY"
BASE COUNT         1076 a         2159 c         2038 g         1269 t
ORIGIN      
        1 ggccggcacc gcaggtattc tcggttctct tcctcgaccc tccatatccc cgcgtcgtgg
       61 atcgcccggc gcgtcttttc ccgcggctgg tgcccatcgt taggggtgag ggcgctcccc
      121 ggtttcgtga tccgccggag ttccgcaagg agcgcgccct gattcccgac cctgcggagc
      181 atgtcgaggg catagatgag gtctgcaacc gtatcagccg gaaatggaga tccgccgtgc
      241 ggtctgcccg ttctggttta cgcgccgata cagattccgg cacctcttta tgaacctggt
      301 gcaatcttct cgcccctcag atcgaactat tcacataagt atgatttttc ataccatatg
      361 aaccccctgt tccagtactt tgctcatccg gcacccgcct caaatgtggt ctcgcggcgc
      421 cttaacgcgg gaccggcagc acccggacac cgggcttcgc ggcttcgcgc gagacggcag
      481 ggtatacgcg atgaagcggc ctctcgcgaa gacgcgaagg gatgttccag gtccctctac
      541 cgaagttcgc gtccttcgcg gctcgcgcga gctcgagggt atggatgatg gaacaacccc
      601 gcgcgaagat gcgaagggga atctgtagtt tgacttctcc gtgggatttt ccggaacttg
      661 agcgaagtgc tgcggtcgtg cgcatcctcc atcatccccg atcaagcggg cagaggcttt
      721 gccccggggc tccggcagac tggggagcac gttgcaaaaa aagtgttatt ctttccggcg
      781 ctccgccttc ttcgccgcca ggcgctcgat ggcggcgata tcgacgttcc cgccggtcgc
      841 gagttttccc ttccagacag cccggtcgac gaaacccctg ccgatatcgt ttcggatcac
      901 gagcgtcgtg tagccgtccg gcgacccgat ggccccggcc gatatatccg catccaccgc
      961 cgtaaggtcg gtgcagaccc ggcacccggg ccggacgcac tcctcgaggt cggcaagcgg
     1021 gatggggatg tgccgcccgt cctgcaggta tacgtccagt ttccccttga cgtccaggcg
     1081 ctggatctcc cagggttcga tcttcttctc ggactgcagc ttctcctcga ccagcttcgc
     1141 gtagtcgaag gtctccgtgc agaagagacc gatgacgagc cggatagcct tcgcgtaggg
     1201 ccggaggaga tcgtggtcgc ttgcccggat cgcctgggtc gcccgcgcca cacagggcac
     1261 gcctacgacg gcgatccgcc ggtacttccg ggtgaccacc gcctccttca aggaggcaag
     1321 cagcggcacc caccaggagt atcggctccc ggcgtgatgg atgagcgcat ccgacgacgt
     1381 gatcaccgcc gaggacggtt tcagcgtcca ggggtctctc gtcaccgtga cgaccgcatc
     1441 gatcagccct tcatcgaggg cgtttgcgag gattgccgtc accgcgcccc cgctctgctt
     1501 gttctccacg gggaaagccg accgggccgc gacgatctcc cggtaggccc cgagcatccg
     1561 cccctgcccc gcaaggtcga cccgggggca ggccgcgtag caggccccgc agggcacgcc
     1621 gtcgttctcg gacttgcagt agccgatgtt caccggcgca tcggtcttcc ccggcacaaa
     1681 ccggagggca tccgccggac agaccgccac gcatgctccg cacccggagc agaggtccgt
     1741 agtccagacc tcggcctcca ggttcttgaa agtcttcgtc tccatcccca tcactcccac
     1801 gtatacttcc cggcaaaggg ccgtgcgctc gcagggacga tccgtgtgta atccgcgttc
     1861 acgagcggtt ccggatcgag cccgaaatcg tccgcaaact cccggatgac cggggcgatc
     1921 cgtgccctgt cctcatcggt gagcggcaat gtcttcgctc ccgcggcgag gcaggcttca
     1981 tccacgttcc cgcggatgac gatctcgccg ccgtggatac cgctcccgat ctcccgggct
     2041 ttcatggccc cgtcgagtgc gaggacgagc agaaggccac ctgccatgta ctcgccgagg
     2101 aacgcctgcg cagacccccc gacgaccagt atcggccgat gggcctcgta ctgcttcatg
     2161 tggatcccgc cccggtagcc gatatccccg cggacgaaga ccttgccgcc ccgcatgctg
     2221 tgcgccaccg cgtcgccggc actcccgtgg atcaccacct tcccggagtc catcgtgttg
     2281 ccgggcgcgt gttcggcgtt cccgtgaacg acgacggtcg ggccgctcat gaacatggcg
     2341 agatccccgc ccgcgacgcc gttgacccgg atgactgcat tcccgcgaag gccgttgccg
     2401 atgaaccgct gcccgaggac gttgtcgacg acgatctcat ccgctccatc ctcgatggcc
     2461 ttcctgatct gctggttcag gggggtgtag tgtacccctt tcgcatcaat cgtgaccaat
     2521 tttgtcattg tgctcacgct cccaccgatt tgacgtcgag gatctcaagg agcccctcgt
     2581 cgagcatgta gccacggagc cggtcgcggt tgccgcgcag gctctcgatg ctgttgatgc
     2641 cggccgcgcc catcagctcg gtgatctcaa gcgtccaggc gtggatcagg tttgccacct
     2701 gctttgatgc cacgtcggga tccaggcgtg cgacaaggtt cggtcgctgg gtggcgatgc
     2761 cccaggagca gaccccctgg taacagtttc cgcagacccg gcagcccatc gccacgagcg
     2821 ccgcggtccc gatgtagacg gcatcggcgc cgagagcgat cgcctttacg acgtctgcgc
     2881 tctcgcggat gctgccgcag gcgatgagtg agacctcgtt cctgagtccc tgcttgcgga
     2941 gttcgcggtc ggcggtcgcg atggcgacct cgatcgggat gccgacgtgg tcgcggaaga
     3001 cccgcggtgc cgcgccggtg ccgccccgga acccgtcgat tgcaacggca tccacgccgg
     3061 aacgggcaat ggccgctacg ttctcggcgt tgttgttgac ggccgcaatc tttgcaaaga
     3121 ccggtttttt ccacccggtc gcctccttga cggagttgac gagctggggg aggtcctcga
     3181 tgccgtagac gtcgtgatgc ggcgcagggc tgatcgcgtc gcttccctcc ggtatcatcc
     3241 ttgtccggga gacgtccgcg ctcaccttct cgccggggag gtgcccgccg atgcccggtt
     3301 ttgctccctg gccgagtttg agctcgatcg cggcgccccg ctccaggtag tcgacgttca
     3361 cgccgaacct ccccgaggcg acctggacga tcatccggtc ctggtagggg tagaggccgg
     3421 ggtgcaggcc gccctcgccg gtgcccatgt aggtgcccgt ctcctttgcc gcccgggcca
     3481 tcgcgaggtg ggcgttgagg ctgattgcgc cgtagctcat gtggccgagc atgatcggcg
     3541 tctcgagcat gaggtttgga gcgagttccg tggagacctc gatatcgccg ttcggttttc
     3601 gcgcaaaatc cagcctcgac ggtttcttcc cgaggtaggt ccggagttcg acgggttcgc
     3661 ggagcgggtc ggtcgggggc gtggtgacct ggcaggcgtc cagcatcagg tggtcgaata
     3721 tgatcgggag gtcggcgacg ctccccatcc cggcgaggat gatcttaccc gtccgggcct
     3781 ggttgtagat agactccctg acctgccgcg accagaccgg atggctccgg taatcgcagg
     3841 ggtgctcctc gagcatgatg gcgtctcgcg ggcagtaggc gaggcaccgg tgacaggcgg
     3901 tgcagttccg ggagttgatg aggatgcggt cgccgtccca tcggaatacg ccgtaggagc
     3961 agttctcgat gcatcgcccg cattccatgc accgctcccg gtcgatggtg acccggtacc
     4021 ggagcggcat gctgccgatg ttgctgctca tggtgcaacc ctcccgatga ccggctctcc
     4081 tgcacccggt ctcgtgatcg actcgacgcc gggctccatg gtacggatcg ccgtctcttc
     4141 gcttgcgatg tagagccggt cgccgcactc gccgacgacc ataggccgga gtttgccccg
     4201 gtcggtgaac ccgaccatca tatcggggtt cgccgccacg acggtgaacg gcccgttcat
     4261 cgtcgcgggg ccgtaggcga accggagggc gcggttcagc ttctgctcgg cttcaggcat
     4321 ccggtcgatc tcgtcccagt agggcggggc gagcgcccgg atggcgaggt cgatctcaag
     4381 gccgtgcctg cgcaccagga agtcgattgt gtaggcgatc acctcgctgt cggtgccggt
     4441 cgagcagttg tagccgaagc tctcgatgta gcgccggttg gccccgtagg tggtggtctt
     4501 cccgttctcc acgatgctcc agtcgtgcag gttgaacggg tcgggccgct cccacctctc
     4561 cgggcagctc gtcgcgtagc ggttgtgtgc gagccagatg tagccctcgt agtcctcgat
     4621 ccggtagtaa tccgcgacgg tctccggcca gctcccggct cctccggcct tgaagacacc
     4681 gacgttcttt ccggacgaga tgacttcggt cccgtctgga cttgcgttga tctgcatcac
     4741 aaggttggtg atgatatcct tctcgggcgt cgcgctcccc ggggcgaggg aggggtcagg
     4801 cttgaagaag tagcgccagg gggtgcaggc tgcccggaag ttcggctggt cgcaagtcgg
     4861 gatcgcctcg tcgtgctcga tcgctcccca ctgctccagc gttgcgtcga cgaccgcctt
     4921 gctctcgcag gcgtggtcga ggaagacgtg gagcgcataa cagtcccggt agtcggggta
     4981 tatgccgtac gccacgtacc ccgccccctc gccgctgccg cgctcgttca ttatggagag
     5041 agcctgccgg atgccggaac cgtccatcat ctggcgtctc ctgtccatta caccaaaaat
     5101 gccgcacatg gtaacctcgg tgaacaacgt tgtattgccg tttcctatag ttctgcctgg
     5161 gtatatttgt gtcaataggt atttattcca tcggatagaa gtagacttcc acatgtcccg
     5221 tgatgccact tccgcgatgc ttgagcgcat cggagaggat aatgttcgat ttcttcacct
     5281 gcagttcacc gacctcctcg gtatgccaaa gaacgtcgcc atcccggtga agcaggcgga
     5341 gaaggcgctg accgggggca tcgggtttga cgggtcgtcg atccaggggt tcgtccggat
     5401 agaggagtcc gatatgatcc tgaaacccga cctctccacc tacaccctcc ttccctggcg
     5461 atcgggggag tacacggtgg cgcgcttcat gtgcgacgtc tacaaggcga acggcaaacc
     5521 cttcgagggc gacccgcgct acgtcctccg cacggccatg gaggacgcgg cacgagacgg
     5581 gtatatattc aacacgggcc cggagctcga gttcttcctc ttccgcacga tcgacggcaa
     5641 accgacgacg ttcttccagg atgccggcgg ctacttcgac cttgcgccga ccgatctcgc
     5701 ggaggacgtc cggcgcgaga tcatcatcgc cctcaccgag atggggtttg agatcgaggc
     5761 ctcccaccac gaggtcgcgg agagtcagca cgagatcgac ttcaagtaca gcgacgccct
     5821 ccataccgcg gacaacgtca tcacgttcaa gttcgtggca aagacgatgg cgctgatgcg
     5881 cggcctgcac gcatcgttca tggcgaaacc tatctacggc atatgcggga gcggcatgca
     5941 caccaactgt tcgcttgcaa agggcggggt gaatgcattc tatgaccccg atgccccacg
     6001 ggagctctca acggcctgca tgcacttcat cggcggcctc ctccggcacg caaaggccat
     6061 cacccgggtc gcgaacccga cgatcaactc ctacaagagg ctcgtccccg gctacgaggc
     6121 accctgttac atcagctgga gcgccggcaa ccgctcggcc ctcgtccggg tcccgacgcc
     6181 gcgcggcaac agcacccggg tcgagttccg cagtcccgat ccgacctgca acccctacct
     6241 cacctttgcc gcgatgctca ccgccgggat ggaaggtgtc cgggagaaga tcgaaccgcc
     6301 ggccggcgtc gataagaata tctaccacat ggcggccgaa gaccggaggt gcgccggcat
     6361 cgagacgctc cccggggatc tttactcggc ccaccaggcc ctgcttgccg acgacctcat
     6421 ctgccgggcg ctcgggcctc acgtcgtcga ggcgctcacg aacgtcgcag aggccgagtg
     6481 ggaggcatac cggacggcgg tccatccgtg ggagcttgag cggtacctgg cgacgtattg
     6541 aa
//
LOCUS       DAOSEE010000118         6393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_4376_length_6393_cov_256.943358, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000118 DAOSEE010000000
VERSION     DAOSEE010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6393
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4376_length_6393_cov_256.943358"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..582
                     /locus_tag="PLI76_08740"
     CDS             112..582
                     /locus_tag="PLI76_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HOI62159.1"
                     /translation="MNRSSAVRRDTGAIEARIGYVFQDRALLLRALTRLAYTLETGLP
                     PGAHMDALATLGDAVINVVVVEALVAEGAHDKGAITNRKVNLVNMTRLRSLAEDLDLE
                     GYVRWGKGEAVQAVWTSGRVLAECLEALVGAVYLDGGMSAAAGVLRRLGLTEKA"
     gene            complement(589..1881)
                     /locus_tag="PLI76_08745"
     CDS             complement(589..1881)
                     /locus_tag="PLI76_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HOI62160.1"
                     /translation="MTETPTIFGLPAERGRWGLIALGLLINLCLGSIYSWSVFVAPLA
                     AHFTAALGREVTAGEVLLPFSVFLAFFAIAMPLAGKYIERYGPRKVTVAGGLLTGLGW
                     LLASTATSVEALYVVYGVIGGLGVGIAYGAPVAVAARWFPDRRGLAVGLTLLGFGVSA
                     FVTANAAGALIAAYGVMTTFAIFGVVLAAVLVVAALPLRFPPAGWHPAGWSAPAPGTG
                     TAPACECDRRAMLRTGTFYGLFACYFVGCLAGLTAISIAKPVGTEVAGVDAGLATLLV
                     GFFAIFNGAGRPAFGGLADRISPQKTAMLTFGLIAAASVLIWLAPGVPAYVVSFAVLW
                     GCLGGWLAIAPAATASYFGTCDYPRCYGVVFLAYGAGAIAGPQLAGFVRTATGTYLGV
                     FPLVAVLAAVGFAVAWLLMRPPASVPASTPVPVAAGEE"
     gene            complement(1888..3777)
                     /gene="acs"
                     /locus_tag="PLI76_08750"
     CDS             complement(1888..3777)
                     /gene="acs"
                     /locus_tag="PLI76_08750"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HOI62161.1"
                     /translation="MTETFAVKLEEKCYLPDPRCKAAAWTPDYTRAYQEFMHDPEAFW
                     DQVAHELDWFEPWDRVLEWNYPYAKWFVDGKLNITYNCLDRHVTSDRRDKPAVIWRGE
                     GGEERRLTYDGLYREVMRCANALAGLGVGKGDRVCIYMPLVPEQIVAMLACARLGAVH
                     SVVFGGFGAGALAMRITDAEAKVVITADVGYRRGKTIPLRDIVNEALTHAPCVEKTVV
                     FRRGTPAVDLDPAREVDYADLVARAAPDCPAEVMDAEDPLFILYTSGSTGAPKGIVHT
                     CGGYMVGTYYTARHDFDIKESDILWCTADPGWITGHSYIVYGPLAVGTTVVIAEGTPD
                     YPDPGAYWRLVQDLGVTIFYTAPTAIRMFMRHGDAWPAKYDLSTLRVLGSVGEPLNPE
                     AFEWYYHRIGGGECPVVDTWWQTETGMHVVTTMIGEAMKPGFAGRAIPGAVVDVVDRT
                     GKPVPPGTGGFLVIREPWPAMLRTVHGNDARYRTYWETIPGCYTAGDLAVKDEDGYIM
                     ILGRADDLIVVAGHNIGTAEVESALVSHDAVAEAAVIGKPDALKGNVIKAFVTLRVGA
                     NPGDALTDELARHVKKSLGPVAVPAEIEFMDRLPKTRSGKIMRRVLKARELGMDPGDI
                     STLEE"
     gene            complement(4159..5100)
                     /locus_tag="PLI76_08755"
     CDS             complement(4159..5100)
                     /locus_tag="PLI76_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOI62162.1"
                     /translation="MFRIYRTVEGATVPRTDETGRFEPGAWVSLVNPTADEIREAAEK
                     LSIPGDYIRAALDEEERPRTESEDGVTLIIIDIPMPYPEQTILYTTMPLGIIVTPDVI
                     VTVCLRENAIIEDFANGRVKLFYTAKKTRFVLQILFRNASYFLQYLRQIERISDRLGA
                     ELNQSMRNRELIQLMNLKKSLVYFSTSLKSNQIVLDKTLTFQPLRMYADDTDLLEDVI
                     IENKQAIEMANTYSTILSETMDAFASIISNNFNNVLKLLTSITIILAIPTMIASFLGM
                     NVPVPLQDEPYGFLIIIALSLIVSSLLAVAINRKGWL"
     gene            complement(5207..>6393)
                     /locus_tag="PLI76_08760"
     CDS             complement(5207..>6393)
                     /locus_tag="PLI76_08760"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOI62163.1"
                     /translation="FVRTNKTESSAIHMAFNKGLLSALVLVVIATYFLVTQILGAEYI
                     GVFYATVAGLIAGFAIGLITEYYTSFDRKPTLSIVKSTETGAATTIITGFAKGMESTI
                     WPVLIVAVAIYISYQVAGLYGIAIAAVGMLSTLGISLSVDAYGPVADNAGGIAEMSHQ
                     KPEVREITDTLDAVGNTTAAIGKGFAIGSAALTALALFSAYTQAVNLSIIDVSVPNVF
                     IGLLIGAMLPFLFCSLTMMAVGKAAYSIVHEVRRQFKEIAGLMEGTVEPDYTSCIAIS
                     THSALREMIVPGILAVVAPLAVGFVLGPEALGGLLVGSLASGFLLAVTMANSGGAWDN
                     AKKYIEQGHLGGKGSPAHKAAVVGDTVGDPFKDTSGPSLNILLKLMSMVALVFAPLIL
                     VV"
BASE COUNT         1137 a         2005 c         2160 g         1091 t
ORIGIN      
        1 aggggatgga tatcgtcgat aagatcggga aggtaaggac ggactccgcc gataaaccga
       61 aggtccccgt ccggatagag aaggcggaag tcctggagta gcccctctgc catgaaccgc
      121 agcagcgccg tccgccggga cacgggcgcg atcgaggcac ggatcggcta cgtattccag
      181 gaccgggcgc ttcttttgcg ggcgctcacc cgcctcgctt acaccctgga gacgggtctt
      241 cccccgggcg cccacatgga tgccctcgcg accctcggcg atgcggtcat caacgtcgtg
      301 gtcgtggaag cgctggtcgc ggagggggcg cacgacaagg gcgcgatcac gaaccggaag
      361 gtgaacctcg tgaacatgac ccgcctccgc tctcttgcag aagacctcga cctcgagggg
      421 tacgtccgct gggggaaggg cgaggccgtc caggcggtct ggacatccgg ccgggtcctt
      481 gccgagtgcc tggaggcgct cgtcggcgcc gtctacctgg acggcggtat gagcgcggcc
      541 gccggggtgc tccggcggct cgggctcacg gagaaggcgt aaccgctttt actcttcccc
      601 tgccgccacc ggcacgggcg tgctcgccgg aacgctcgcc ggcgggcgca tcaggagcca
      661 ggcgacggca aaccccaccg cggccaggac cgcaaccagc gggaagacgc cgaggtaggt
      721 tccggtcgcc gtccggacaa acccggcaag ttgcggcccg gcgatggcac ccgcgccgta
      781 ggcaaggaag acgaccccgt aacaccgcgg gtagtcgcac gtcccgaagt aggaggccgt
      841 cgccgccggc gcgatcgcaa gccacccccc aaggcatccc cagagaacgg caaacgagac
      901 gacgtaggcg ggcacccccg gtgcgagcca gatcaggacc gatgccgccg cgatcaggcc
      961 aaacgtcagc atcgccgtct tttgcgggga tatccggtcg gcaaggccgc cgaacgccgg
     1021 cctgcccgcc ccgttgaata tggcgaagaa cccgacgagg agcgtcgcga gccccgcatc
     1081 caccccggcg acctcggtcc cgaccggctt tgctatcgat atcgcggtga gccccgcgag
     1141 gcagccgacg aagtagcagg cgaagaggcc gtagaaggtc cccgtccgga gcatagcgcg
     1201 gcggtcgcac tcgcaggccg gggccgtgcc cgtgccgggc gcaggcgccg accaccccgc
     1261 cgggtgccag cctgccggcg ggaaccggag cggcagggcc gcaaccacca gcacggcggc
     1321 gaggacgacg ccgaatatcg caaacgtcgt catcacgccg taggcagcga tcagggctcc
     1381 tgcggcgttt gcggtgacga acgccgagac cccgaacccg agcagggtga gcccgaccgc
     1441 gagcccccgc cggtccggga accaccgggc cgcgacggcg accggcgccc cgtaggcgat
     1501 gccgacaccg agccccccta tcaccccgta gacgacgtag agggcctcga ccgacgtcgc
     1561 cgtcgaggcg aggagccacc cgaggccggt gaggagcccg ccggcgaccg tcaccttccg
     1621 cggcccgtag cgttcgatat acttcccggc gagcggcatc gctatagcga agaaggcgag
     1681 gaagaccgag aacgggagga ggacctcccc cgccgtcacc tcccggccga gcgctgcagt
     1741 gaagtgcgcc gcaagcggcg cgacgaagac gctccaggag tatatgctcc cgaggcagag
     1801 gttgatgagg aggccgaggg cgatgagccc ccacctcccc ctctccgccg gcagcccaaa
     1861 gatcgtcggt gtctctgtca tgcccgtcta ctcctcgagt gtcgatatgt cgcccgggtc
     1921 catgccgagc tcgcgggctt tcaagacccg gcgcatgatc ttgccgctcc gggtctttgg
     1981 gagccgatcc atgaactcga tctcggccgg caccgcaacg ggcccgaggc tcttcttgac
     2041 gtggcgggcg agttcgtcgg tgagggcgtc gccgggattt gcgcccacgc ggagggtgac
     2101 gaacgccttg atgacgttcc ctttcagggc atcgggcttc ccgatgaccg cggcctccgc
     2161 caccgcatcg tgggagacga gcgcgctctc gacctccgcg gtcccgatgt tgtgcccggc
     2221 gacgacgatc aggtcgtcgg cccggccgag gatcatgatg tagccgtcct cgtccttcac
     2281 cgcgaggtct cctgcagtgt agcagcccgg gatggtctcc cagtaggtgc ggtagcgcgc
     2341 atcgttcccg tggaccgtcc ggagcatggc cggccagggc tccctgatga ccaggaaccc
     2401 cccggtgccg ggcgggaccg gcttacccgt ccggtcgacg acatccacga cggcgcccgg
     2461 tatggctctt ccggcaaacc cgggtttcat cgcctcgccg atcatcgtgg tgacgacgtg
     2521 cataccggtc tcggtctgcc accaggtatc cacgaccggg cactctccgc cgccgatccg
     2581 gtggtagtac cactcgaagg cctcggggtt gaggggttcg ccgacggagc cgagcacgcg
     2641 cagcgttgaa aggtcgtact tcgccggcca cgcatcaccg tggcgcatga acatgcggat
     2701 tgcggtcgga gcggtataga agatcgtcac cccgaggtcc tggaccagcc gccagtaggc
     2761 gcccgggtcc gggtagtcgg gtgtcccttc ggcgatgacg acggtcgtcc cgacggcaag
     2821 cgggccgtag acgatgtagc tgtggccggt gatccacccg gggtcggcgg tgcaccagag
     2881 gatatcgctc tccttgatgt cgaagtcgtg gcgcgcggtg tagtaggtcc cgaccatgta
     2941 gccgccgcag gtatggacga tacccttcgg tgcgccggtc gagccgctcg tgtagaggat
     3001 gaagagcgga tcttccgcat ccatcacctc cgccgggcag tccggtgcgg cccgggccac
     3061 aagatcggcg tagtcgacct cccgcgcggg gtcgaggtcg acggccggtg tcccccgccg
     3121 gaagacgacg gtcttttcga cgcacggcgc gtgggtgagc gcctcgttca cgatatcgcg
     3181 gagcgggatg gtctttcccc gccggtagcc gacatctgcg gtgatgacga cctttgcctc
     3241 cgcatcggtg atccgcatcg cgagcgcgcc cgcgccgaac ccgccgaaga ccaccgaatg
     3301 gacggccccg agccgcgcgc aggcaagcat cgcgacgatc tgctcgggga cgagcggcat
     3361 gtagatgcag acccggtcgc ctttcccgac gccgaggccc gcaagggcgt ttgcacaccg
     3421 catcacctcg cggtagaggc cgtcgtaggt gagtctccgc tcttccccgc cctcgccgcg
     3481 ccatatgacc gccggtttat ccctgcggtc gctcgtgacg tggcggtcga ggcagttgta
     3541 ggtgatgttg agttttccgt cgacgaacca cttcgcgtag gggtagttcc actcaagaac
     3601 ccgatcccag ggctcgaacc agtcgagttc gtgggcgacc tggtcccaga aggcctccgg
     3661 gtcgtgcatg aactcctggt acgcccgggt gtagtccggc gtccaggcgg ctgccttgca
     3721 ccgggggtcg gggaggtagc acttctcctc cagtttgaca gcaaatgtct ctgtcatatc
     3781 tcctcattcc tatcttctgt catgccgggg tgcacggccg ggacccgcgc gggtcacatt
     3841 accgtgcagt gccggtcgat ccgttaggtc tgccggtctt tcggcacctg atccgtggtc
     3901 gatacatgcc ggggatgggc aacgggtgcc ggccccggcg ggtgattccc catcacgggc
     3961 aaccccctat tttacatgat ctatcatgtt gatttatcat gatcaataat gtataattcc
     4021 gggataaact ttgcggtcag gcgggcgacg gggttgagat catgcacctg ccggtgcttc
     4081 ttgcaaggcc gcgatgagga tatgcttgcg ccgggtgccg cggatggccg gcacccggcg
     4141 cgggcccgga cgatcggcct acaaccatcc tttccggttg atcgccaccg caaggaggga
     4201 ggagacgatc agagagaggg cgatgatgat gagaaatccg tagggctcgt cctgcagggg
     4261 gaccgggacg ttcatgccga ggaaacttgc gatcatcgtc gggatggcga ggatgatggt
     4321 tatcgacgtg aggagtttta gaacgttatt gaagttgttc gatatgatcg aggcgaacgc
     4381 atccatagtc tccgagagga tcgtcgagta ggtgttcgcc atctcaatcg cctgcttgtt
     4441 ctcgatgatg acgtcctcga ggagatcggt gtcgtccgcg tacatccgga gcggttgaaa
     4501 cgtcagggtc ttgtcgagga cgatctggtt gctcttgagc gatgtcgaga agtagaccag
     4561 gctctttttg aggttcatca gctggatgag ctcccggttc cgcatcgact ggttcagctc
     4621 ggctccgagc cgatccgata tccgctcgat ctggcgcagg tactggagga aataagatgc
     4681 atttcgaaag aggatctgga gcacgaaccg ggtctttttc gcggtgtaaa agagtttcac
     4741 ccggccgttt gcgaaatcct cgatgatcgc gttctcgcgg aggcagacgg tgacgatgac
     4801 gtcgggggtg acgatgatcc caaggggcat cgtcgtgtag aggatcgtct gctccgggta
     4861 gggcatcggg atatcgatga tgatcagggt gacgccgtct tcgctctcgg ttcgcgggcg
     4921 ttcctcctcg tcgagcgccg cccggatata atcgcccggg atggaaagtt tctctgcggc
     4981 ctctctgatc tcatccgcgg tggggttgac gagagagacc caggcgcccg gttcaaaccg
     5041 cccggtctcg tccgtccgcg gcaccgtggc cccctcaacc gtacggtaga tccggaacat
     5101 gcggtcttca cccccgctcc agggtatctg cagtatctgg ttatccgtag aacgggatga
     5161 aggtatgtcc tggctccgct cccggcggac cttaaaaaaa ggtgagttag acgaccagga
     5221 tcagcggtgc aaagaccagc gcgaccatag acatcagctt caagagaatg ttcagggacg
     5281 ggcccgacgt atccttgaag ggatcgccca cggtgtcgcc gacgaccgct gccttgtggg
     5341 ccggcgatcc cttcccgccg aggtggccct gttcgatgta cttcttcgcg ttgtcccagg
     5401 ccccgccgga gttcgccatc gtgacggcga ggaggaaccc ggatgcaagc gaaccgacga
     5461 gcaggccgcc gagcgcttca ggcccgagaa cgaacccgac cgcgagcggt gcgacgacgg
     5521 cgaggatgcc gggcacgatc atctcgcgca gcgccgagtg ggtggatatg gcgatgcagg
     5581 aggtgtagtc gggctcgacc gtcccctcca tgaggccggc gatctccttg aactggcggc
     5641 ggacctcgtg gacgatgctg tacgcggcct tccccactgc catcatcgtg agagagcaga
     5701 agaggaaggg gagcatcgca ccgatcagga ggccgatgaa gacgttcgga accgagacgt
     5761 cgatgatcga gaggttgacc gcctgggtgt aggcggagaa gagggcgagc gccgtcagag
     5821 ccgcagagcc gatggcgaac cccttgccga tagcggcggt ggtgttgccg acagcgtcga
     5881 gcgtatcggt gatctcgcgg acttcgggtt tctggtgcga catctcggcg ataccgccgg
     5941 cgttgtcggc gaccgggccg taggcgtcga cggagaggga gatcccgagc gtcgagagca
     6001 tgccgacggc ggcgatggcg ataccgtaga gcccggcgac ctggtaggat atgtagatcg
     6061 cgacggcgac gatcaggacc ggccagatgg tgctctccat cccctttgca aacccggtga
     6121 tgatggtggt cgcggccccg gtctcggtgg acttcacgat tgaaagggtc ggtttccggt
     6181 caaatgaggt gtagtactcg gtgatcagac cgatagcgaa ccctgcgatg agaccggcga
     6241 cggtggcgta gaagacgccg atgtactccg ccccgaggat ctgggtcacc aggaaataag
     6301 tggcgatgac gaccaggaca agcgcggaga gcaacccttt gttgaaggcc atgtggatgg
     6361 cgctgctctc ggtcttgttc gtccggacga aga
//
LOCUS       DAOSEE010000119         6286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_4478_length_6286_cov_201.885091, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000119 DAOSEE010000000
VERSION     DAOSEE010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6286
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4478_length_6286_cov_201.885091"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PLI76_08765"
     CDS             complement(<1..290)
                     /locus_tag="PLI76_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1616 domain-containing protein"
                     /protein_id="HOI62164.1"
                     /translation="MPPEASPGPLSGPPRTCTVLPGLTGSCPDASLSIAAQGESDDLS
                     GGSAPRFERVLSLVLIAAVLAALGTTIFIIVSPGEGEKFTEFYILGPTGKAA"
     gene            533..1090
                     /locus_tag="PLI76_08770"
     CDS             533..1090
                     /locus_tag="PLI76_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOI62165.1"
                     /translation="MSREGKNEQAFTGLEAAIIFIAFVVVASVFSYVVLGMGMTTSQK
                     SQEVVHAALDEARSAFRPGDAVIVKLDVAGNLSFIEFDLETAAGPTAIDMKSMTCTLA
                     TSKALLTYPPGDPAVKLTWRYCKDTDDILEAGEIVTVRLTPGSKGIKRGDTFTIGVVA
                     AGGATASLTRTIPAGARGNTYVELF"
     gene            1074..1649
                     /locus_tag="PLI76_08775"
     CDS             1074..1649
                     /locus_tag="PLI76_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOI62166.1"
                     /translation="MSSSSDAGFTGLEAAIVLIAFVIVAAVFGFVILQAGFTSAQQGQ
                     SVIHDGMEQAGSSCMVSGIVYGISARPGVVESFVIPVGLTAGGEPIDMATVSVRFTGP
                     GHSNPISRSVPLADTFPKPGYWSIQERVNSDSDLLLEPGERFILNVSPEVWKDCQPYG
                     SFAIEIKPPGRAALRVERKIPGSIERVNRLE"
     gene            complement(1654..2340)
                     /locus_tag="PLI76_08780"
     CDS             complement(1654..2340)
                     /locus_tag="PLI76_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOI62167.1"
                     /translation="MFVACHLLLGLIIGLVIARHLGDRRLIGLAALGAILPDLIDKPV
                     GHILLAESLNSGRLFTHGLLFLALLLMAGIILERRRGSFALLAVAAGVLSHQILDAMW
                     AAPVAWYFPFLGPYQPHEFADYFGNAILAEVSSLSEWIFLAAAAAIILALSRGTRPGF
                     LAIARTLARVAIPLLILLTLASLYAWAAGIPESILMAGGSGEDYLLLAAVGAAGIAGM
                     VRYRDLLDLG"
     gene            2437..2625
                     /locus_tag="PLI76_08785"
     CDS             2437..2625
                     /locus_tag="PLI76_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62168.1"
                     /translation="MASFEREILVLFVSFLIGSVAGWWSRVYWESSLAVVAATLAGTV
                     AGYCIITVALRAAGHPVA"
     gene            complement(2610..3323)
                     /locus_tag="PLI76_08790"
     CDS             complement(2610..3323)
                     /locus_tag="PLI76_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5714 domain-containing protein"
                     /protein_id="HOI62169.1"
                     /translation="MEHRAGCLICGRDILYSEEPRDLPCAVCGEIRSTTAACEAGHFV
                     CDRCHSLEALDLIEEFCIATDLEDPLAVACILMRCPAVRMHGPEHHFLVPASLIAAYC
                     NHRGEPGRKAPLVRQARARAGMVPGGFCGSHGTCGAAIGTGIFVSLITGSTPLKKQEW
                     SLANQMTARSLMTIARHGGPRCCKRDSWLAIRAAVTFLSERFGITLPMPDAVRCEFSD
                     INRECLREACPFHAGYATG"
     gene            complement(3313..5169)
                     /locus_tag="PLI76_08795"
     CDS             complement(3313..5169)
                     /locus_tag="PLI76_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI62170.1"
                     /translation="MSITDCYRRSIAFTRDILRQPAKTGGGLRFSDIAFFLRFARPIR
                     RAGALALVTTALVSAEKTVLPLSLKIFVDNILLGEAVAPDALLAGTGGILSSLNILVA
                     ALLVLGLLTGATDLARNYWTARFREGYAFNLQTALFEHVLGFPLSYFKSQQTGYIMSR
                     LSDDVQILQYTVSQYLPQIVANALYAAFTLVILGALNIRLTLVVLAFIPLYILVNAVF
                     IRRIRAAVHRERERQAYVSRDLAEVISGIDTVKSHAAEDREVHRVSGTLRNMVDARIR
                     STMLSAFSEYFRVGAMSLMLIAVIWFGGNDVLAGAMTVGDFVAFAVYIMTFAPTVSTF
                     FAFPVVMQPAATSAARLRELFAMQGEPGALPDAGGFVPDTVQGRIEFSRVWFGYGEDT
                     PVLRDAQFVIRPGEVVAVVGRTGAGKTTLINLILRAFSPEKGTITLDGRDLSTLDPRW
                     LRRQISIVSQDLFLFHTTIEENIRYSRPDATAGDVVEAAEKAQIHDDIVRLPDGYQTV
                     VGERGARLSVGQRQRIAIARAFLKDAPILILDEPTSALDLRTEDRIRQTLRDLVRDRT
                     TILVSHRPSLLEIADRVLTIEDGQVRECRMPAPPSLPDLFKGCGDAPLRHGA"
     gene            5314..6066
                     /locus_tag="PLI76_08800"
     CDS             5314..6066
                     /locus_tag="PLI76_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62171.1"
                     /translation="MVSRAAADRWKGAVVGILAVGAVFLLGRFAILPAPVMTVAFLFG
                     GGLAAGLFTPGTVRDGAITGAACGALVALLASFGITLASLVNSEPYYPPFWMTFGFYA
                     LILTSLLLPYNAIGGAAGTAVRNGVGGGEHAGRGRWFGIGVGTAIIAASALFLFFVGP
                     FVVAAPLAGGFIAGFFAGDKPGDGFEAGLVAALFGTGLFSLPLLWTASYGTGFAAGLA
                     GMAAIALGYIFISFGTAAGVAGAVVRASHGTA"
BASE COUNT         1101 a         1889 c         2210 g         1086 t
ORIGIN      
        1 tcggctgcct tccccgtcgg cccgaggatg tagaactcgg tgaacttctc cccctctccg
       61 ggggagacga tgatgaagat cgtcgtcccg agagccgcga ggacggcggc gatgaggacg
      121 aggctcagga cccgctcgaa ccggggggcg gaaccgccgg agaggtcgtc agattcgccc
      181 tgtgccgcga tcgagaggga tgcatcgggg caactgcccg ttagacccgg caggacggtg
      241 caggtgcgcg gggggccgga gagaggaccc ggagaggctt caggaggcat tctgttaccc
      301 catgataggc caggtataaa taggtttccg gcggcagacc ctcgccccgt atatcgatcc
      361 gggagaaccg ggagcctcac gaacagaggg tgccgaacgg attttgccct ctctcccgga
      421 gtgcgcccgg gcacatatct cgtcacggct gccctcctgc cggccgcaaa atcctgctat
      481 ctccgggtga taccagcatg ttttaatcac ggtacgccca aacccgatca ttatgtcccg
      541 ggaggggaag aatgagcagg ccttcaccgg ccttgaggcg gctatcatct tcatcgcctt
      601 cgtcgtcgtt gcatcggtct tctcctacgt cgttctcggg atgggcatga cgacgtcgca
      661 gaagagccag gaggtggtgc acgccgccct cgacgaggcc agatctgctt ttcgccccgg
      721 ggatgcagtg atcgtaaaac tggatgtggc gggtaacctc tcgttcatcg agttcgacct
      781 cgagaccgcg gccggaccga ccgccatcga tatgaagagc atgacctgca ccctcgccac
      841 atcaaaggcg ctcctcacgt atcctcccgg tgaccctgcg gtaaagctca cctggcgata
      901 ttgcaaggac accgacgata tactggaggc gggggagatc gtcacggtga ggttgacccc
      961 gggcagtaag ggcatcaaac gcggagatac gttcaccatt ggagtggtcg ccgcgggagg
     1021 tgcgaccgct tcgctgacga gaacaattcc tgccggcgcc agggggaata cctatgtcga
     1081 gctcttctga tgccgggttc accgggcttg aggcggcgat cgtcctgatc gcgttcgtca
     1141 tcgttgccgc ggtcttcggg ttcgtcatcc tgcaggccgg tttcacctcc gcacagcagg
     1201 gccagtcggt catccacgac ggtatggagc aggcgggatc gagctgcatg gtgagcggca
     1261 ttgtctacgg catcagcgcc aggccagggg ttgtggagtc gttcgtcatc ccggtcggcc
     1321 tgacggccgg gggcgaaccg atcgatatgg ccacggtatc cgtccggttc accggtcccg
     1381 gacatagcaa tcccatctcc cggagtgtcc ctctggccga caccttcccc aaacccgggt
     1441 actggagcat ccaggagagg gtgaacagcg attccgacct gcttcttgag ccgggagaac
     1501 ggtttatact gaacgtatcg ccggaggtct ggaaagactg ccagccctac gggtcgtttg
     1561 ccatcgagat caaaccgccc ggacgggcgg cgctccgggt cgagcggaag attcccggca
     1621 gcatcgagcg ggtgaaccgg ctggagtgac ggcctacccg agatcgagga ggtcacggta
     1681 ccggaccatc cccgcaattc cggcggctcc cactgccgca agcagcaggt agtcttcccc
     1741 gctgcccccg gccatcagga tgctctccgg tatgccggcc gcccaggcgt agagggaagc
     1801 gagagtgagg agtataagga gcgggattgc cacgcgggcg agcgtgcggg cgatcgcgag
     1861 aaagcccggc cgggtgcccc gggagagagc aagaatgatc gcggccgcgg cggcaaggaa
     1921 gatccactcc gagagcgacg agacctctgc aagtatggcg ttgccgaagt agtcggcgaa
     1981 ctcatgcggt tggtacgggc cgaggaacgg gaagtaccag gcgacggggg ccgcccacat
     2041 cgcatcgagg atctggtggg agagcacgcc cgccgcgacg gcaaggagcg caaacgatcc
     2101 tcgtcgccgt tcaaggatga tgccggccat aaggaggagc gcaaggaaga gcaacccgtg
     2161 tgtgaacagc ctgccggagt tgagcgactc tgcaagcagg atgtggccca ccggtttgtc
     2221 gatcaggtcg gggaggatgg ccccgagagc ggcaagcccg atgagtcgcc ggtcgccgag
     2281 gtgccgggca atcacgaggc cgatgatgag gccgagcagg aggtggcagg cgacgaacat
     2341 ccctcatgag atcggtgccc ggccgggata taaccctgct atccgtgcgc aaacgattat
     2401 gtccggagag aaccgacggt tgaagggaga gcggtcgtgg cctcattcga gagagagatc
     2461 ctcgtactct ttgtttcgtt cctcatcggc agcgtagcgg gctggtggag ccgtgtatac
     2521 tgggagagca gcctcgccgt cgttgccgcg accctcgccg ggaccgtggc cggttactgc
     2581 atcatcaccg tcgccctccg ggccgcgggt catcccgtcg cgtagccggc atggaagggg
     2641 cacgcctccc gcaggcactc gcggttgata tcgctaaact cgcaccgcac cgcatccggc
     2701 atggggaggg ttatgccgaa ccgttccgag aggaacgtga ccgccgcccg gatcgcaagc
     2761 cacgagtcgc gtttgcagca ccggggaccc ccgtggcggg cgatcgtcat caggctgcgt
     2821 gcggtcatct ggtttgcaag cgaccactcc tgcttcttca agggcgtcga tcccgtgatc
     2881 aggctcacga agatgcccgt cccgatcgcc gcgccgcagg tgccgtggct gccgcagaac
     2941 cctcccggaa ccatgccggc cctcgcacgc gcctgccgga cgagcggggc tttcctcccc
     3001 ggctcccccc ggtggttgca gtacgcggcg atgaggcttg ccggcacgag gaagtggtgc
     3061 tccggcccgt gcatcctgac cgccgggcag cgcatcagga tacaggcgac ggcaagcgga
     3121 tcctcaaggt ccgttgcgat gcagaactct tcgatgaggt cgagcgcctc aaggctgtgg
     3181 caccggtcgc agacgaagtg cccggcctcg caggccgccg tggtgctccg tatctccccg
     3241 cagacggcgc aggggagatc gcggggttct tcgctgtaca ggatatcccg gccgcagatg
     3301 aggcaccctg ctctatgctc catgtcgtag gggtgcgtcg ccgcacccct taaagagatc
     3361 cgggagagag ggaggggcgg gcatgcggca ctccctgacc tgcccatcct cgatcgtgag
     3421 gacccggtcg gcgatctcca ggagcgaggg gcggtgggag acgaggatgg tcgtccggtc
     3481 ccggaccagg tcgcggagcg tctgccggat ccggtcctcg gttcggaggt cgagtgcgga
     3541 cgtgggttcg tcgagtatga ggatgggcgc atccttcagg aacgcccggg cgatggcgat
     3601 ccgctgccgc tggccgacgg agagccgggc cccgcgttcg ccgacgaccg tctggtagcc
     3661 gtccgggagc cggacgatat cgtcgtggat ctgtgccttc tctgcggcct cgacgacatc
     3721 cccggcggtg gcatcaggtc tgctgtagcg gatgttctcc tcgatggtgg tgtgaaagag
     3781 gaagaggtcc tgcgagacga tcgatatctg ccgccgcagc caccgggggt cgagcgtcga
     3841 gaggtcgcgc ccgtcgagcg tgatcgtgcc tttctcgggc gagaacgccc ggaggatcag
     3901 gttgatgagc gtcgtcttcc cggcgcccgt ccgcccgacg acggcgacca cctcgccggg
     3961 ccggatgacg aactgcgcat cccggaggac cggggtatcc tctccgtaac cgaaccagac
     4021 gcgggagaac tctatgcgcc cctgcacggt gtcgggaaca aacccgccgg cgtcggggag
     4081 ggcgcccggt tcgccctgca tcgcaaagag ctcgcgcagg cgggcggccg acgtcgccgc
     4141 gggctgcatc accacgggaa acgcgaagaa ggtgctgacg gtgggggcaa acgtcatgat
     4201 atagaccgcg aacgcgacga agtcccccac ggtcatggcg ccggcgagca catcgtttcc
     4261 gccgaaccat atgacggcga tcagcatgag cgacatcgcc ccgaccctga agtactccga
     4321 gaacgcggag agcatggtgc tcctgatccg ggcgtcgacc atgttccgga gcgtccccga
     4381 gaccctgtgc acctcccggt cctctgcagc gtgcgatttg acggtatcga taccggatat
     4441 cacctccgcg aggtcccgcg agacgtaggc ctgccgttcc cgctcccggt ggacggcggc
     4501 acggatcctt cggatgaaga cggcgttgac caggatgtag agggggatga aggcgaggac
     4561 gacgagggtc agcctgatgt ttaaggcgcc gagtatgacg agggtgaagg cggcgtagag
     4621 ggcgtttgcg acgatctggg ggaggtactg ggagacggta tactgcagga tctggacgtc
     4681 gtcggagagg cgggacatga tgtagccggt ctgctgcgac ttgaagtagg agagcgggaa
     4741 accgaggacg tgctcgaaga gggcggtctg gaggttgaag gcgtagcctt cgcggaaccg
     4801 ggcggtccag tagttccggg cgaggtctgt tgcgccggtg aggaggccga gcacgaggag
     4861 ggcggccacg agtatattga gcgacgagag gatgccgccg gtaccggcaa ggagggcgtc
     4921 gggtgccacg gcctccccga gcaggatgtt gtcgacgaag atcttgaggc tgagcgggag
     4981 gacggtcttt tcggcggaga caagcgccgt cgtcacgaga gcgagagcgc ccgctctccg
     5041 gatcggccgg gcgaaccgga ggaagaaggc gatgtcggag aaccggagtc ctccgccggt
     5101 cttcgccggc tggcgcagga tatcccgcgt gaaggcgatg gagcgccgat agcagtctgt
     5161 gatactcata gctggtccgg ttgaggagcg ataaacccgg ggccggccgc atctccgttg
     5221 cagttgcacg ccctcgtatc gtataatacc tgcggccccg gtatactgga ggcaaaccga
     5281 aacggataac atatggcggg gtgagactct catatggtct cgcgtgccgc cgccgatcgg
     5341 tggaaggggg cggttgtggg aatcctggcg gtcggtgcgg tctttcttct cggccggttt
     5401 gctatcctcc cggccccggt catgacggtg gcgtttctct tcggcggggg ccttgccgcc
     5461 gggctcttca ccccggggac cgtcagggac ggtgccatca ccggtgccgc gtgcggggcg
     5521 ctcgtggccc ttctcgcgag ttttggtata accctcgcga gccttgtaaa cagcgaaccc
     5581 tactatcctc cgttctggat gacctttggt ttctacgccc tgatcctcac gtcgctcctc
     5641 cttccctaca atgctatcgg cggagcggcg ggaacggcgg tgcgaaacgg cgtcggggga
     5701 ggcgagcacg ccggacgggg ccggtggttc ggcatcggcg tcggcacggc catcatcgct
     5761 gcatcagccc tcttcctctt cttcgtcggc ccgttcgtcg tggccgctcc gctcgccggc
     5821 gggttcatcg cggggttctt tgccggggat aaacccgggg acggttttga ggcagggctt
     5881 gtcgcggcgc tcttcgggac cggcctcttc tcgctccccc tcctctggac ggcatcctat
     5941 ggcacggggt ttgccgccgg gcttgccggg atggccgcga tcgctctcgg gtatatcttc
     6001 atatccttcg ggacagccgc cggggttgcc ggggcggtcg tgcgggcgag tcacgggacg
     6061 gcgtgagggc gtttgcgggg agatgaagcg gtcgggatga ccgctccggg agcgcctgca
     6121 ggcaggggaa cttatagatg tccgtctgcg gggagaggcg gaccctccct tctcatggcg
     6181 atagccgcac gatctgcttc gtgagaagag cctggaggcg cgggcggggt gggggatgcc
     6241 ggcagaagta aggtatttgg agcgtgggag acaggggagg gggaac
//
LOCUS       DAOSEE010000120         6237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_4520_length_6237_cov_224.124555, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000120 DAOSEE010000000
VERSION     DAOSEE010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6237
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4520_length_6237_cov_224.124555"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..925
                     /locus_tag="PLI76_08805"
     CDS             77..925
                     /locus_tag="PLI76_08805"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOI62172.1"
                     /translation="MVLVGCHVSIAGSIDLAVGRALDSGCDTFQIFSRNPRGWKAKDI
                     DPDVAGAFRRALDASGIGPAVDHMPYLPNLASPDDAIYEKSVAALTDELRRCEVLGIP
                     CLVTHLGHHRGAGTGTGQERVVAAINRALEEAGAQGVMLLLENTAGEKNSVGSTVADL
                     CRIFEGIDGKERIGICFDTCHAFAAGYDLRTGEGVDAVLEEIDDLIGPGHLRIIHLND
                     CKGDLGSGLDRHEHIGLGSIGEEGFRHILSRPAIRGCPLICETPVDGRRGDDGNIAKV
                     RELAGA"
     gene            1216..4386
                     /locus_tag="PLI76_08810"
     CDS             1216..4386
                     /locus_tag="PLI76_08810"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOI62173.1"
                     /translation="MIVCTVIAAGLLASPVLAVEAAPGETVLFCTAGGSQEHPDIDGG
                     MVVWEDGRDDRSIYYAAAPGSGGRKVAGGYAEERYPSVSGDFIAWQDRRHGNWEIYLF
                     SPYDGERRITNSTTDQTMPIVHGNHIAWYDTRKGYTDICLYDIATGKETYLGCSPRIE
                     WKLALSERYVVWEEIAGGIQAYDIQTEEKLGIAASGARQAYPAISGDRIVWEDYRNGL
                     PDIYMYDLNEGREYQITDDPAEQVSPAIDGEIIAWEDKRSGIWDIYMYDLRAWTEMSV
                     CTANGEQLFPSVSGEKIVWQDKRSGRWQVYLFAYSGGAPPEAEFSADPIAGTVPLTVR
                     FADRSTGSPDEWEWEFGDGGSSTEPNPGHTYEDPGEYTVSLRVSNRFGSDIVTKTDLI
                     RARTLEPPAADFTAAPVSGSLPLTVRFADRSTGAPSAWLWDFGDGATSNEQDPEHTYA
                     AVGTYAVSLTAGNPAGSTNMTKPAFITVVSPPKASFSADTRSGRAPLAVRFSDASTGT
                     ITAWSWDFGDGGSSHDQNPTHTYTGPGIYTVALTAANEYDSDTRTMEGYITVTAPPVA
                     GFSANVTVGRSPLAVMFSDESGGGPLSWSWTFGDGASSAEKSPVHVYQAPGAYTVTLT
                     VANGAGENTTARTGYITVRDLLSARFTANVTAGAAPLAVLFTDASVGEPRSWSWDFGD
                     KGTSTVPAPVHVFEQPGNYTVSLTIADGADRSTKSLQIRAFGPPGAEFSAAPLNGTAP
                     LTVAFTDASSGEPSAWLWDFGDQGRSIERHPSHTYTAAGNYTVNLTVENAAGTDTATK
                     TGYVSVHPAPVTPAPTSGGGGGGGGGGGGGGGGGGGGSTWTTPDPGTPTATATPTATP
                     SPGPAGPDVLPLGESGLVSRFVRIASPDGVASLAIAEGVRAVDAVGGPLRTVTLKLLD
                     SPDVPGAPGSYVFSGYACTAGPDGAAFSPPATLVFNLTEEEWDAVYSSRRGFLVQWYN
                     RSANAWEEVPTLVHPETRSVTAEVSHFSLYALFVGLPGDGAVEVIAPDTPQAGEAFPS
                     MQIIPGLIALAIVGAGAYLYYRRGEP"
     gene            complement(4431..5639)
                     /locus_tag="PLI76_08815"
     CDS             complement(4431..5639)
                     /locus_tag="PLI76_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62174.1"
                     /translation="MIDSMTGIYRYFRNRGPTMAALLIAVAMIAVVPVSGAALPVAWN
                     ATFSPEENSKFDAVAGTADGGYIALGSVLAGSGEDLLLVKTDGRGSEVWTERFPGMAA
                     ASVAETADGGYIAAGYNATAEPEQNAYQGSSLLIRTDAAGKEEWRQVLQGEKVSAVRP
                     TADGGYVIAGWLWDPPGSANDTTAIVTKTDGSGTPTWNRTFPGAAANAGIVTADGGYI
                     IGGTKSPFAYDIGDAFLIRLDTAGNTLWEKNYAVPVIFDVEETDEGGFVHSGNFWYGL
                     VDAKGNEVWLRNMEGLAGYAVTLRPAGGYMIAGTDIRRGEAFAFGTDGDGAIRWNTTI
                     PDTRVYAAESTPGGYVLAGIRHLSPASGAAWLAGLEEAAEPAPTTPGFGAIAAGAALL
                     LLFAGRKRRG"
     gene            5760..>6237
                     /gene="endA"
                     /locus_tag="PLI76_08820"
     CDS             5760..>6237
                     /gene="endA"
                     /locus_tag="PLI76_08820"
                     /EC_number="4.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000214 - tRNA-intron endonuclease
                     complex [Evidence IEA];
                     GO_function: GO:0000213 - tRNA-intron endonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006388 - tRNA splicing, via
                     endonucleolytic cleavage and ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-intron lyase"
                     /protein_id="HOI62175.1"
                     /translation="MLATFDGTWVRLGGEGRTFYEQGGYGRPEGTSLRLSPEEALYLM
                     ERGKIDVKDFDFDALLGLFAGQPNFVRRYLVYRDIRERGYVVQPGPHDFRVFRRGHKP
                     GVGRSQYLIRVLSERDLVDFEQLGQDVLAAVNMRKQYLLAVVDDEDELTYYEVRVQD"
BASE COUNT         1081 a         2053 c         2016 g         1087 t
ORIGIN      
        1 cgggagttcg gggcggcggt gctccgcgag gtcgggcggg ggaaggccta aacgccctcc
       61 cgccaaggtg taggctatgg tgctggtagg atgccacgtc tccatcgcgg gctcgatcga
      121 ccttgcggtc gggcgggccc tcgattcggg ctgcgatacg ttccagatct tttcaagaaa
      181 cccccggggc tggaaggcaa aagacatcga ccctgacgtg gccggtgctt tccggagagc
      241 gctcgacgca tccggcatcg gtccggccgt cgaccacatg ccttacctcc cgaacctggc
      301 ctcgcccgac gatgcgatct acgaaaaatc ggtcgcggcg ctcaccgacg aactccggcg
      361 gtgcgaggtg ctcggtatac cctgcctcgt gacgcatctt ggccatcacc gcggcgccgg
      421 gaccgggacc gggcaggaac gggtcgtcgc cgccatcaac cgggcgcttg aagaggccgg
      481 cgcacaaggc gtgatgctcc tcctcgagaa caccgccggc gagaagaaca gcgtcgggtc
      541 gaccgtcgcg gacctctgcc ggatctttga gggtatcgac gggaaggaga ggatcgggat
      601 atgtttcgat acctgccacg cgtttgccgc aggctacgac ctccgaaccg gggaaggggt
      661 cgatgcggtg ctcgaggaga tcgacgacct gatcggcccg gggcacctcc ggatcatcca
      721 cctcaacgac tgtaagggcg acctcgggag cgggcttgac cggcacgagc atatcgggct
      781 tgggtccatc ggggaggagg ggttccggca tatcctcagc cgtccggcca taaggggatg
      841 tccgctcatc tgcgagacgc cggtggatgg acggcgggga gacgatggaa atatcgcgaa
      901 ggtccgcgaa ctcgccgggg catgaatttc tctgcgatac cctatcctca taatattcgc
      961 taatttttat aataattacg aatacttctc tcggctcccc ggggccgcaa gggatatata
     1021 tttatacccc catcatgccg ttcacgaacc ctttgcccgg caggacccgg tgactctccg
     1081 gcatcctccc ggacctcagt ttgcacgcgg gcacggccgg gaagcttctc tctcctgcct
     1141 gcaggcacca tcgtgtgaga cgaaaggggg cgttctcatc atggtgatcg aggaatggct
     1201 gaatacagag tgttaatgat agtctgcacc gtcatcgccg ccggactgct ggcctcgccc
     1261 gttctggcgg tggaggcagc gccgggggag acggtgctgt tctgcaccgc cggcggatcc
     1321 caggagcacc cggatatcga cggcggtatg gtcgtctggg aggacggcag ggacgatcgg
     1381 tctatctact acgctgccgc tcccggcagc ggtggccgga aggttgccgg cggctacgcc
     1441 gaagagcggt atccgtccgt gtcgggcgat ttcatcgcct ggcaggaccg gcggcacggg
     1501 aactgggaga tctacctctt ctcgccatac gacggcgaaa ggcggatcac gaacagcacg
     1561 accgaccaga cgatgcccat cgtgcacggt aaccatattg cctggtacga tacgcgaaag
     1621 gggtataccg atatctgcct ctacgacatc gcgaccggca aggagacgta ccttggctgc
     1681 tcgccgagaa ttgagtggaa actcgctctc tcggagcgtt acgtcgtctg ggaggagatc
     1741 gccgggggca tccaggcata cgatatccag accgaggaga aattggggat agcggcgagc
     1801 ggtgcgcggc aggcataccc tgcgatatcg ggggaccgga ttgtctggga agattaccgg
     1861 aacgggctgc ccgatatcta catgtacgat ctcaatgagg gccgggaata ccagatcacc
     1921 gacgaccccg ccgagcaggt ctctccggcg atcgatggag agatcatcgc ctgggaggac
     1981 aagcggagcg ggatctggga tatctacatg tacgatctcc gcgcctggac cgagatgtcg
     2041 gtctgcaccg caaacggcga gcagctcttc ccctcggtct ccggggagaa gatcgtctgg
     2101 caggataagc ggagtgggag gtggcaggtc tacctcttcg cctactccgg cggagcgccg
     2161 ccggaggcgg agttctcggc ggatcccatc gccggcacgg tcccgctcac cgtccggttc
     2221 gccgaccggt ctaccggcag tccggatgag tgggagtggg agttcggcga tggcggttcg
     2281 tcgacggagc cgaaccccgg gcatacctat gaggatcccg gcgagtacac ggtctctctt
     2341 cgggtgagca accgcttcgg gtccgacatc gtcacaaaga ccgatctcat ccgcgcaaga
     2401 accctggaac ccccggccgc cgatttcacc gccgcaccgg tgtcgggcag tctccctctc
     2461 accgtgcggt ttgcggaccg atcgaccggc gcgccatcgg cctggctctg ggactttgga
     2521 gacggcgcga cgtcaaacga gcaagacccg gagcacacct atgccgcagt cggcacctac
     2581 gccgtcagcc tgaccgccgg gaacccggcg ggcagcacga acatgacgaa acccgccttc
     2641 atcaccgtcg tcagtcctcc aaaagcctcg ttctcggcag atacccggag cgggagagcc
     2701 ccgctcgccg tccggttctc ggacgcctcg accggcacta taacggcctg gtcctgggat
     2761 ttcggcgacg gcgggtcctc tcacgaccag aaccccaccc acacctacac cgggccgggc
     2821 atctacaccg tcgcgctcac ggccgcaaac gagtacgaca gcgatacccg gacgatggaa
     2881 ggctacatca ccgtcaccgc accgccggta gccgggttct ccgcgaacgt gaccgtcggt
     2941 cgctcgccgc tcgcggtgat gttctcggat gagtccggag gaggtccgtt atcgtggtcc
     3001 tggaccttcg gcgacggggc ctcatcggca gagaaaagcc cggttcatgt ctaccaggcg
     3061 cccggggcct acacggtcac tctcaccgtg gcaaacggcg caggggagaa cacgacggcc
     3121 cggaccggct acataacggt tcgcgaccta ttgagcgctc ggtttaccgc caacgtgacc
     3181 gccggggccg ccccgctcgc ggtcctcttc accgacgcgt cggtcggcga accccggtcg
     3241 tggtcgtggg acttcggcga taaggggacg tcgaccgtgc cggccccggt ccacgtattc
     3301 gagcaacccg gtaactacac cgtcagcctg acgatcgccg acggcgccga tcgttcgacg
     3361 aaatccctgc agatccgcgc ctttggtccg ccgggtgcag agttcagtgc cgctcccctg
     3421 aacggcacgg cccccctcac ggttgcgttc accgacgctt cttccgggga gccctccgcc
     3481 tggctctggg acttcggcga tcaggggcga tcgatcgagc gccatccctc gcacacctac
     3541 accgccgccg ggaactatac cgtcaacctg accgtggaga acgccgccgg gacggacacc
     3601 gcgacgaaga ccgggtacgt atccgttcac cccgccccgg taacgccggc accgacgagc
     3661 ggcggcggtg gcggcggtgg aggaggtgga ggaggtggcg gtggcggcgg tggcggagga
     3721 tccacctgga cgaccccgga cccgggcacg ccgaccgcga ctgcgacccc gactgcgacc
     3781 ccctcccccg ggcctgccgg acccgacgtg ctccccctcg gggagagcgg ccttgtgagc
     3841 cggttcgtcc ggatcgcttc gcccgacggt gttgcaagcc tcgccatcgc ggagggtgtc
     3901 agggccgtcg atgccgttgg aggcccgctt cgcacggtga ccctcaaact cctcgattca
     3961 cccgacgtgc ccggggcgcc gggatcatat gtgttcagcg ggtatgcctg cactgccggg
     4021 ccggatgggg ccgccttctc accgccggcg accctggtat tcaacctcac cgaggaggag
     4081 tgggatgctg tctacagcag ccggaggggc ttcctggtgc agtggtataa ccggtcggcg
     4141 aacgcctggg aggaggttcc caccctcgtt caccctgaaa ccaggagcgt cacggcagaa
     4201 gtctcgcact tcagcctgta tgcgctcttc gtcggtctgc ccggcgacgg cgcggtagag
     4261 gttatcgcgc cggatacccc gcaggccgga gaggcgttcc cctccatgca gatcatcccg
     4321 ggattgattg cccttgccat cgtgggtgca ggggcatacc tctactaccg gagaggggag
     4381 ccctgaacag cgggtctccc cgcccgtaaa aatgcgcgtg cgggaagggt ttaccccctc
     4441 cgcttccttc ccgcgaacag gaggaggagg gccgccccgg cggcgatcgc gccaaatccc
     4501 ggtgttgtcg gtgccggctc cgcggcctcc tcgaggccgg ccagccaggc ggcgcccgat
     4561 gcgggcgaga ggtgccggat gcccgcaagg acgtagccgc cgggggtgct ctccgctgcg
     4621 tagaccctcg tatccgggat cgtcgtgttc caccggatcg ccccgtcccc gtcggtcccg
     4681 aaggcgaagg cctcccctct cctgatgtcg gtgcccgcta tcatgtatcc cccggcaggc
     4741 cgcagggtga cggcgtagcc ggcaagcccc tccatgttcc ggagccagac ctcatttcct
     4801 tttgcatcga cgaggccgta ccagaagttc cccgagtgga caaacccgcc ctcgtcggtc
     4861 tcttcaacat cgaagatgac cgggacggcg tagttcttct cccagagcgt gttgccggca
     4921 gtgtcgagac ggatgaggaa tgcatccccg atatcgtagg cgaagggcga cttcgtgccg
     4981 ccgatgatgt atcccccgtc agcagtcacg atccctgcgt tcgcagcggc tccggggaat
     5041 gtccggttcc aggtgggcgt gccgctgccg tcggtcttcg tgacgatcgc ggtcgtatcg
     5101 tttgccgatc cgggcgggtc ccagagccat ccggctatga cgtagccgcc gtcggcggtc
     5161 ggcctgaccg ctgagacctt ctcgccctgc aggacctgcc gccactcttc cttcccggcg
     5221 gcatcggtcc tgatcaggag cgagctgccc tggtaggcgt tctgctccgg ttcggccgta
     5281 gcgttgtatc cggcggcgat gtaaccgccg tcggcggtct ctgcaaccga agctgccgcc
     5341 atgccgggga acctctccgt ccatacttcg ctcccccggc cgtcggtctt tacgagcagc
     5401 agatcttccc cggacccggc gaggaccgag ccgagggcga tataaccgcc gtccgccgtt
     5461 cctgcgacgg cgtcgaactt gctgttctcc tcaggcgaga acgttgcatt ccaggcgacc
     5521 ggcagagcgg ccccgcttac cgggacgacg gcgatcatcg ccaccgcgat aagcagcgcc
     5581 gccatggtcg gcccccggtt ccggaagtat cggtatattc ccgtcatgga atcgatcact
     5641 ggtgcggttt aacacgccaa aaatttagtg gttctctctc acccgggccg ggaggtgtgc
     5701 cgatgcgccg gcgcgccggc aatcccttaa agtacacaaa ccccctataa ctctaccacg
     5761 tgttggcgac attcgacgga acctgggtgc gccttggtgg cgaaggacgc acgttctacg
     5821 agcagggggg gtacgggagg cctgagggga ccagcctccg gctctctccg gaagaggcgc
     5881 tctacctcat ggagaggggc aagatcgacg tgaaagactt cgacttcgac gccctgctcg
     5941 gtctctttgc cggccagccg aattttgtcc gccggtatct cgtctaccgc gacatccgcg
     6001 aacggggata cgttgtccag cccggcccgc acgatttccg cgtcttccgc cgcggccaca
     6061 aacccggggt cggcaggtcc cagtacctga tccgtgtcct ctccgagcgg gaccttgtcg
     6121 atttcgagca gctgggccag gacgtgcttg ccgcggtcaa catgaggaaa cagtacctcc
     6181 ttgcggtcgt ggacgacgag gacgaactga cctactacga ggtgcgggtg caggacc
//
LOCUS       DAOSEE010000121         6133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_4616_length_6133_cov_194.902435, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000121 DAOSEE010000000
VERSION     DAOSEE010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6133
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4616_length_6133_cov_194.902435"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..237
                     /locus_tag="PLI76_08825"
     CDS             76..237
                     /locus_tag="PLI76_08825"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HOI62176.1"
                     /translation="MEVMQHDPTVTIAELARIIGVTNRAIKKQIEKLKQQGRLRRVGP
                     ARGGRWEVL"
     gene            237..704
                     /locus_tag="PLI76_08830"
     CDS             237..704
                     /locus_tag="PLI76_08830"
                     /inference="COORDINATES: protein motif:HMM:NF016220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HOI62177.1"
                     /translation="MTFTEANTVEQLILDAVTRKRQAARLTVQEAPRAGSPGDIHPAG
                     WTYVPAREIPRRPGDVMVESWLRDALIRLNPRIAAEPGRADEVIYNLRAILLAVQADG
                     LVRANENFMAWLRGEKTMPFGKNGEHVPVRLVDADNPGGNHLVVTNQWTYPAR"
     gene            683..2800
                     /locus_tag="PLI76_08835"
     CDS             683..2800
                     /locus_tag="PLI76_08835"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HOI62178.1"
                     /translation="MDLPGPIEKRFDIVFVVNGLPLVIGEAKSPARSAVTWFDGAYRV
                     SEIYEKQVPAIFVPNVFSFATEGRVYRYGSIRMPIDLWGPWRTEENEPEGTLADVSRS
                     VESMLQPEVVLDLLQYYTLFATDKKHRRIKIIARYQQFVTANQIVDRVVRGYPKKGLI
                     WHFQGSGKSLLMVFAAQKLRLHPGLGNPTVMIVVDRIDLDTQITATFNAADVPNMVSV
                     ATRQELQTLLAQDVRKVLITTIHKFAEADGDLNERSNIIVLVDEAHRTQEGDLGRKMR
                     EALPNAFLFGLTGTPINRADRNTFWAFGADEDEKGYMSRYSFQESIRDRATLPLHFET
                     PEVKLQIDRAAIDEAYQAMYLDKPMKDHNLLQAICRTNRTFGQKKTHGLIVDYIGIFD
                     DVAHALDFDEKTVQQVITNIDDLRRELPIRVQKCLTFFPGVDRTRGGYEGLMEAQQCL
                     PDNEARDGFAAEYSVLGTIWEALSPDPVLGPYENDFRWITQVYESVRPPSGHGRLLWH
                     ALGAKTVDLINKNVHVQSVRDDVETLVLDAQVLAGVLGDADPEKRAREIEIKLIARLR
                     RHAANPAFIALGERLEKVRDRHEQGFLTSLELLKAILEVAKDVVEAERETDPEEERDR
                     AREALTELFNEAKNKATHIIVERIVTDIDDIAKKVRFPDWQHTSQGERLVQKELRRTL
                     LKYKLHTDQELFDRAYAYIRQYC"
     gene            3209..4852
                     /locus_tag="PLI76_08840"
     CDS             3209..4852
                     /locus_tag="PLI76_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HOI62179.1"
                     /translation="MKNIGDETEDLDPGTKENVARALWMSGYAPESIPPDVMSSLCGT
                     KNHRFGDLMRRRLADLLANPERFTPDYTERYTTFCNHARDLSAHQAYAMTNLLGLDSA
                     RGYQELPQEITFTFPCDDRPQFEYQVGWHFFVGTASDAGGREFGIQFMLWSYSLLPPE
                     MARDEGLSDIENQIVEIHLAVTPANDRHHRPRPVLVAGTTGLIRFSENPYEYAVGKNT
                     MTSLADDSLFPVRLRARGIDEREDAPVEIAIDLTLHQTKGYILNGDGGLAPSCGGVGT
                     LYYSVTNLRIRPGESWLSIDGTRVPLTGGKFWYDHQWGTGFMPPGSPRSDLLRAVGHL
                     HEQGPGGWDWMAIQFDDDTEIALSALHTNDNRAFYSQTGAKPPTMAAGAKGSYIRQNG
                     EYESITAEIRVTDWIRSAVADGPYLATNTWYPNRVEVTVYEDAVPAKKRHFVMVPIVT
                     TGQQGFFAAGSEYSEGAVTIESADGERIGIGFLESTGYIDARRQGLLLAGLPDTDDMI
                     RLVSPPAVPDEMKAEVLALFQDPEIVAKMGEELAKCKGL"
     gene            5161..5913
                     /locus_tag="PLI76_08845"
     CDS             5161..5913
                     /locus_tag="PLI76_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62180.1"
                     /translation="MDIEAFFAHEGVDVSSRVGIEDLTDADRASVREFLPAARSVIVF
                     GREVPAPVYRMPEREKTREMLRIAASLDETASRLAGCLDAEHIPARVIPLYFPVRVVD
                     EKVQGIVRLKQIAAAAGLGDIGKNTLLLHRRFGPRLLLAGVVTGEPASGKTAAAAPVC
                     TGCGACIRACPAGALGPAGVDAFRCRNVRAWVPPPVVPAAKWLLRRQFLLRSAAPLAP
                     LVARAATIRCSRCVTECPMFPGEEQAPTFHAE"
     gene            complement(5938..>6133)
                     /locus_tag="PLI76_08850"
     CDS             complement(5938..>6133)
                     /locus_tag="PLI76_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62181.1"
                     /translation="TWRRRTIRAGYSRPILPGGRRSGEEEMVQAVIQPPTPVDLLQVV
                     YCCGEEYRLLQEPRIHGMVA"
BASE COUNT         1250 a         2001 c         1855 g         1027 t
ORIGIN      
        1 agttccccaa aaagttcccc aaaaagttcc ccaaaaagtt ccccaaaaag ttccccaaaa
       61 agttcccaga agatcatcga agtcatgcag catgacccaa ccgtgaccat tgccgaactc
      121 gcccggatca taggcgtaac caaccgggcg atcaagaagc agatcgaaaa attgaaacaa
      181 caaggccggc tccgtcgcgt cggccctgcc cggggcggcc gctgggaggt cctctaatga
      241 ccttcaccga agcgaacacc gtcgagcagc tgatcctcga tgccgtcacc cggaagcggc
      301 aggccgcccg cctgaccgtg caggaagccc cccgggccgg atcgcccggc gacatccacc
      361 cggccggctg gacctacgtg cccgctagag agatcccgcg ccggccgggc gacgtgatgg
      421 tcgagtcatg gctgcgggac gcgctcatcc gtctgaaccc ccggatcgcc gccgaacccg
      481 gccgggccga cgaggtcatc tacaacctcc gcgccatcct ccttgccgtg caggccgacg
      541 gcctcgtccg ggcgaacgag aacttcatgg cctggctccg gggcgagaag accatgccct
      601 tcgggaagaa cggcgaacac gtcccggtgc ggctcgtcga tgccgataac ccgggcggta
      661 accaccttgt cgtcaccaac cagtggacct acccggcccg atagagaagc ggttcgatat
      721 cgtcttcgtc gtcaacggcc tcccgctggt catcggcgag gcaaaaagcc ccgcccggag
      781 cgccgtgacc tggtttgacg gcgcataccg ggtcagcgag atctacgaga agcaggtgcc
      841 ggcaatcttc gtgccgaacg tcttctcctt cgccaccgag gggagggtct accgctacgg
      901 ctccatcagg atgcccatcg acctctgggg cccgtggcgg acggaggaga acgaaccgga
      961 ggggacgctt gccgacgtga gccggtcggt cgagagcatg cttcagcccg aggttgtgct
     1021 cgacctcctg cagtactaca ccctctttgc caccgacaag aagcaccgcc gcatcaagat
     1081 catcgcccgc taccagcagt tcgtcaccgc caaccagatc gtggaccggg tcgtcagggg
     1141 ctacccgaag aaagggctca tctggcactt ccagggcagc ggcaaatcgc tcctcatggt
     1201 ctttgccgcg cagaaactcc gcctgcaccc gggactcggc aaccccacgg tgatgatcgt
     1261 cgtcgatcgc atcgacctcg acacccagat caccgccacc ttcaacgccg cagacgtccc
     1321 gaacatggtc agcgtcgcca cccggcagga actccagacc ctcctcgcac aggacgtccg
     1381 gaaagtgctc atcaccacca tccacaagtt cgccgaagcc gacggagacc tgaacgaacg
     1441 ctcgaacatc attgttctgg tcgatgaggc gcaccgcacc caggagggag atctcggccg
     1501 taagatgcgc gaggcgctgc cgaacgcgtt cctcttcggc ctcaccggga cgcccatcaa
     1561 ccgggccgac aggaacacgt tctgggcatt cggcgccgat gaagacgaga agggctacat
     1621 gagccgttac tcgttccagg agtccatccg cgaccgggcc accctgccgc tgcacttcga
     1681 gacccccgaa gtgaagttac agatcgaccg ggcggcgatc gacgaggcct accaggcgat
     1741 gtatctcgac aaaccgatga aggaccataa cctcctgcag gccatctgcc ggacgaatcg
     1801 caccttcggc cagaagaaga ctcacggcct catcgtggac tatatcggca tcttcgatga
     1861 cgtggctcat gcgctcgact tcgacgagaa aaccgtgcag caggtgatca caaacatcga
     1921 cgacctgcga agggagctcc cgatccgggt gcagaagtgc ctcaccttct tccccggggt
     1981 ggatcggaca aggggcggct acgaagggct catggaagcg cagcaatgtc tgcccgacaa
     2041 cgaggcacgc gatgggtttg cggcggagta ctccgtgctc ggcaccatct gggaggcact
     2101 ctcgccggac cccgtgctcg ggccgtacga gaacgacttc cggtggatca cgcaggtcta
     2161 cgaatccgtc aggcccccaa gcggccacgg gaggctgctc tggcacgccc tcggcgcaaa
     2221 gaccgtcgat ttgatcaaca aaaacgtcca cgtccagtca gtgcgggacg atgtcgagac
     2281 gctggtgctg gacgcgcagg tgctcgccgg ggtcctgggc gacgccgatc cggagaagag
     2341 agcccgcgag atcgagatca agctcatcgc ccggctgcgc aggcatgcgg caaacccggc
     2401 attcatcgcg ctgggcgaac ggcttgaaaa ggtgagagat cgccatgagc agggtttcct
     2461 caccagcctg gagctcctca aagccatcct cgaggtggca aaagacgtcg tcgaggccga
     2521 acgggagacc gacccggagg aggagcgcga ccgggccagg gaagccctca ccgagctctt
     2581 caacgaggca aagaacaaag ccacccacat cattgtcgag cggatcgtga ccgacatcga
     2641 cgatatcgcg aagaaagtgc gtttccccga ctggcagcat acctcgcagg gcgagcgtct
     2701 ggtccagaag gaactccggc gcactctcct caagtacaaa ctccacaccg accaggaact
     2761 cttcgatcga gcctacgcct acatccggca gtattgctaa tgaccggagc aatcctgtta
     2821 ttctcgatgg tgatcgtcag cgtcgcctcc gctttgttga accaccggca tttgtctcta
     2881 ccgatttacg gcggtatgca taccaccaaa acaggttccc ctccaaaagc cggatcgtgg
     2941 tggctccgga gaacccgggg ccgccgggcc ctctcatcac gggtaccgag atctggggcc
     3001 ggccgcctct gtagacgacg aacgagcggc cccgggcagc agacctcctc acggttgagg
     3061 gcgatcgcct gcagggtttc gcctgcccgt gacgggccgg cataaccggg agagcgtaca
     3121 gttcccggct ggaattcata cccaccccgg gggagccggt aaaagaatat gagctgatat
     3181 aaatgcagag aggattacat aaaaacatgt gaagaacatc ggtgatgaaa cggaggatct
     3241 cgaccccgga acgaaagaga acgttgctcg tgcgctctgg atgagcggat acgcaccaga
     3301 atcgatcccg ccggatgtca tgagcagcct ctgcggcacg aaaaaccatc gattcgggga
     3361 tctgatgcgg cggcgtcttg ccgatctgct ggccaatccc gaacggttca cccccgatta
     3421 caccgaacgg tacactactt tctgcaacca tgctcgtgat ctctcggcac accaggccta
     3481 tgccatgaca aacctccttg gcctggatag cgcacgggga taccaggagc tgccccagga
     3541 gatcacgttc acgttcccct gcgacgacag gccgcagttc gagtatcagg tcggatggca
     3601 ctttttcgtg ggaacggcgt ccgacgcagg tggccgggag ttcggcatcc agttcatgct
     3661 ctggagttac tctctcctcc ccccggagat ggcccgggac gagggcctga gcgacataga
     3721 gaaccagatc gtcgagatcc acctggccgt aactccggca aacgaccggc atcatcgacc
     3781 gaggcccgtt cttgtcgcgg gcaccaccgg cctcatccgg ttctcggaga acccgtacga
     3841 gtatgccgtc ggcaagaaca cgatgacctc tcttgccgat gactccctct tccccgtccg
     3901 cctgagagcc cggggtatcg atgaacggga agacgccccc gtggagatcg ccattgatct
     3961 cacccttcac cagaccaaag gctacattct gaacggggat ggaggcctgg cacccagttg
     4021 cggcggtgtc ggcacgctct actactcggt cacgaacctc cggatacgac ccggcgagag
     4081 ctggctctcc atcgacggca cccgggtccc gctcaccggc ggaaagttct ggtacgatca
     4141 ccagtggggc acgggcttca tgcccccggg aagcccccgg agcgatctac tgcgtgcagt
     4201 cgggcacctc cacgagcagg gccccggggg gtgggactgg atggcgatcc agtttgacga
     4261 cgatactgag atcgccctct ccgcgctcca caccaacgat aaccgggcgt tctattcaca
     4321 gaccggagca aaaccgccga cgatggctgc aggtgccaaa ggatcgtaca tccggcagaa
     4381 cggagagtat gaatcgatca ccgccgagat ccgggtgacc gactggatcc ggagcgcggt
     4441 ggcggacggc ccgtacctgg ccaccaacac ctggtacccg aaccgcgtgg aggtgacggt
     4501 atacgaggat gccgttccag ccaagaaacg gcactttgtg atggtgccta tcgtaacgac
     4561 cgggcagcag gggttctttg ccgcgggttc cgagtactcc gagggcgccg tcaccatcga
     4621 gtctgccgac ggagagagga tagggatcgg atttctagag tctaccggct acatcgatgc
     4681 ccggaggcag ggcctgctcc ttgcgggcct gcccgatacc gacgatatga tccggctcgt
     4741 ctccccgccc gcggttccgg atgagatgaa ggctgaggta ctggcgttgt tccaggatcc
     4801 ggagatcgtc gcgaagatgg gggaggagct ggcgaagtgc aaggggttgt gagcccttcc
     4861 cgccggtcaa acctcatccg gagccatcat cccgcaggta gctgcatcag tgaatcagga
     4921 agagggatcg agcacgcgta agtcgttcct cggtgcgcta caccgggggt ttcccgggtg
     4981 gtcgccccga aggctgcggt gaccggaccg ggaagtattt tttggtaact gcccggtaat
     5041 cttcacccgg tccagcccag accccttctt ctccagggtc ttgatgacta tccggggcgg
     5101 gaacgcctca ccaatacctt gataaggcgt tcgcgcccat gcactccagg aagtgccgga
     5161 atggatatcg aggcgttctt tgcgcacgaa ggagtcgacg tcagctcccg ggtcggcatc
     5221 gaggatctca ccgacgcgga cagggcatcg gtgcgggagt tcctcccggc cgcccggtcc
     5281 gtgatcgtct tcgggaggga agtcccggcc ccggtctacc ggatgccgga aagagagaag
     5341 acccgtgaga tgctccggat tgcggcaagc ctggacgaga ccgccagccg gcttgccggc
     5401 tgcctcgacg cagagcatat accggcccgc gtcatcccgc tctacttccc cgtgcgggtc
     5461 gtcgacgaaa aagtgcaggg tatcgtccgc ttgaaacaga tagcggcggc cgcggggctc
     5521 ggggatatcg ggaagaacac cctcctcctc catcgccggt tcggcccgcg gctgctgctt
     5581 gccggtgtcg tgacgggaga gccagcctcc ggcaagactg ccgccgctgc accggtctgc
     5641 accgggtgcg gggcctgcat ccgggcatgc ccggccgggg cgctggggcc ggccggtgtc
     5701 gatgcgttcc ggtgccggaa cgtgcgtgca tgggttccac ccccggttgt cccggccgca
     5761 aaatggctgc tccggcggca gttcctcctc agaagtgcgg ccccgcttgc gcccctggtc
     5821 gcccgggcgg caacgatccg gtgcagccgc tgcgtcaccg aatgcccgat gttccccggg
     5881 gaagagcagg cgccgacctt tcacgcggag taacccctgc actcccggta ggatctctca
     5941 ggcaaccatg ccatggatcc ggggctcttg caacagccgg tactcttcac cgcaacagta
     6001 tactacctgc aggaggtcta ccggggttgg cggttgtatg accgcctgca ccatttcctc
     6061 ctcaccagat ctcctgcccc ccggcaaaat gggccgtgaa tatcctgccc ggatcgttcg
     6121 tctcctccac gtt
//
LOCUS       DAOSEE010000122         6108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_4642_length_6108_cov_262.996365, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000122 DAOSEE010000000
VERSION     DAOSEE010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6108
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4642_length_6108_cov_262.996365"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            271..1512
                     /locus_tag="PLI76_08855"
     CDS             271..1512
                     /locus_tag="PLI76_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HOI62182.1"
                     /translation="MAVTLPKNFSEVKRHLQEPLIRNSFFIMASSLAAAGFGFFFWMI
                     AARLYSQAEVGVATALMSSMGLLILISRLGLDQSVIRFFPSRDKNGVLGTAIIVPTAV
                     ALGAGLIFIAAVEVISPELAVVRTVAPLYLAFLGAYSVTWVLEGAFNALRKSEHYFAL
                     NLLYGSRILFLIPLVFLGAMGIFSAFGLSFVLGLALALVLLARCSIRPTPGIDRVFLR
                     EAWQFSAGAYIAGMLISAPNMIIPIMVLHVLGAESTASYYITYAIVSILFMIPYAFTT
                     SLFVEGSHGGEMKRSVFRTLASMFALLIPAIIGLSLFGEQILNLIGKDYVEGVALLQV
                     LALSALFVSVCQTFISIAKVRSDIKSLILLSGFVSVALLGLGYTLMQRFGLIGMGYAW
                     LGTYAAGTLLVGLILKRKGWM"
     gene            complement(1602..3809)
                     /locus_tag="PLI76_08860"
     CDS             complement(1602..3809)
                     /locus_tag="PLI76_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2206 domain-containing protein"
                     /protein_id="HOI62183.1"
                     /translation="MRLPDPFQMNDWENRRFFWAFQAIQLAFIGIVCLDLIGYHIPVA
                     REALAFVYLTFLPGILVLKALRLHDLGTIETVLYSVGLSLVVLMLTGLFANTVYPLLG
                     SARPLSLEVLFPTIVGVVQILLYLALTRDREHAGSSHAGLEISIPPPAVPFLALLPFL
                     AIVGTYIRNEYHMVTLLFLLLVLIAVIGLVVGFDRFIPASCYPFAVYSLALALLYHTS
                     LISGYIWGYDIHHELHLASNVLISGTWDMTIPYNTNGMLSVVMLAPIYSLICDLDLVW
                     VFKILYPLFFALVPLALYRVIEKQTNPKIGFFSVFFFMSFFTFYTEMISLARQQVAEL
                     FLVLTVLAMVDKSMDRSRRAFLMITFGFAMIVSHYGLSYIYLFSLVPAWLLLILPDYL
                     PSGILEKLRGMAGTLAHDPLASHEPRPRLQTLVLPYIFILAILTYLWYSTVAEGTAFA
                     TITGIGDKIVNTLFAEPFNPATIQGMYILASQSVTPLHSLAKVVHIATQGLIAVGLLA
                     TLARRGRWRIEPEYLAISIVFFLVNIAGIAVPFFASSLNTSRLYHITLILLAPFAIIG
                     GIALYEAVAGRVRAITALPLMRTAFQALSVFFVVFLLFNTGLIYQVTGDSPTSMALET
                     SGDKPVFNNKEVQGARWLISEGSGRPIYVDGMRWWLLLGFNPDNQRYLPANASYIEPG
                     SYLYLGTYNLVRESVRIEVRERAVTAADYTDAGRFTENQNRIYDNAGSSVYYR"
     gene            complement(4026..5201)
                     /locus_tag="PLI76_08865"
     CDS             complement(4026..5201)
                     /locus_tag="PLI76_08865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOI62184.1"
                     /translation="MRVACLTFWFYDYTIQMANELARHTEVLLLLPDYRTDEYLESID
                     PEVKIRIFRYSRYAGRLGPSCYPVIRDIADAIHDFRPDIVHFQVNNPMLCPLIPMLRR
                     YPLVATFHDIEPHPGEDRLLDIGSLLYRLTLFMSRALTDRMFVHGRALKDILVANYRV
                     PEERVHVIPIGEHEVAPFVKFEEEGREPEGLRVLFFGRIHRYKGLDCLIQAEPLITQK
                     IPGARIVIAGTGEDFGRYEEMMADRDVFEVYNYRIPYEEGARLFQQASVVALPYIEAS
                     QSGVIPTAYGFKRPVVVTDTGSLPEIVDDGETGYVVPPRDPEALAGAIVRLLEDPAAC
                     RRMGERGYMKLKTDMAWPTIVRSILPVYRDLAPAPGREETPADRTFTGRAPAAGKER"
     gene            5705..>6108
                     /locus_tag="PLI76_08870"
     CDS             5705..>6108
                     /locus_tag="PLI76_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62185.1"
                     /translation="MIDRRYVPRLVALLLVCCLIPAFSPVSAAGSATPATLTVAANDS
                     TELSKTQADYVCDGVDDHAEIQAALAALPEGGKVVLSNGTFNCAGVIAPAGGTTLSGQ
                     GPDATNLVFTRDGRITIDREHVTLDGFGVKGSG"
BASE COUNT         1162 a         1793 c         1936 g         1217 t
ORIGIN      
        1 ccttcgcagg aatacccata cgcttctctc cgctttcctc cccgtagagg atacccgtac
       61 gcttctgccg gcccccctct ccatcaggct tcgggcatgc gcccggtgag acctcacggg
      121 gcggtttggt ggagtatcga tcgtgcggag gtctcccggg cttgttttgg gcggcagagc
      181 ctcgcagaac ccccctcgcc cgccctgcaa cctgcaggcg gccgggacgg tggatacatc
      241 tatataggta ccgcccgata atggcggccg atggcagtaa cgctccccaa aaacttcagt
      301 gaagtcaaac ggcacctgca ggaaccgctc atccggaatt cgttcttcat catggcgtcg
      361 tcgcttgccg cggcgggatt cggatttttc ttctggatga tcgcggcacg actctactcg
      421 caggcggagg tgggcgttgc aaccgccttg atgtcgtcca tggggctcct catactcata
      481 tcccgtctcg gcctggacca gtcggtgatc cggttctttc ccagccgcga caagaacggg
      541 gtgctcggga ccgccatcat cgtgccgaca gcggtggcgc tcggtgcagg gctgatcttc
      601 atcgccgcgg tcgaggtcat atcgcccgag cttgcggtcg tcaggacggt cgcgccactc
      661 tatcttgcct tcctcggggc atactccgtc acctgggttc ttgagggtgc gttcaacgct
      721 ctccggaaat cggaacacta tttcgccttg aacctgctct atggatcgcg gatcctcttt
      781 ctcatccccc tggtcttcct cggggcgatg gggatcttca gcgcctttgg cctctcgttc
      841 gtcctcggtc tcgcccttgc cctcgtcctc cttgcccggt gctccatacg gccgacaccg
      901 ggtatcgacc gggtcttcct gcgggaggcg tggcagttct ccgccggcgc atacatcgcc
      961 gggatgctga tctccgcccc gaacatgatc atcccgatca tggtgctcca cgtgctcggg
     1021 gccgagagca ccgcgagtta ctacatcacc tacgccatcg tctcgatcct cttcatgatc
     1081 ccgtacgcgt tcacgacatc gctcttcgtt gaggggagcc acggggggga gatgaagagg
     1141 agcgtgttcc ggacgctcgc gagcatgttc gccctgctca tacctgcgat catcgggctt
     1201 tccctcttcg gggaacagat cctcaacctg atcggcaaag attacgtcga aggggtggcc
     1261 ttgctccagg tgctcgcgct atccgccctg ttcgtctccg tctgccagac tttcatatcc
     1321 atcgccaagg tccggagcga tatcaaaagc ctcatcctcc tcagcggatt cgtcagcgtc
     1381 gccctgctcg ggctcggtta caccctcatg cagcggttcg gcctcatcgg catgggctac
     1441 gcgtggctcg gcacgtatgc ggcaggcacg ctcctcgtcg gccttatcct gaagagaaaa
     1501 gggtggatgt aacttcgggg tgcaggccgg gaggtctctt ccccctccgg cacccccgga
     1561 aatccgtgaa acccggcttt ggccacacgg tggccgcccc ctcaccggta gtagacggag
     1621 gaaccggcgt tgtcatagat ccggttctgg ttctcggtga acctcccggc atcggtgtag
     1681 tccgccgcag tcaccgcccg ctcccgcacc tcgatccgga cgctctcccg cacgaggtta
     1741 tacgtaccga ggtagaggta ggagcccggt tctatatatg acgcatttgc cgggagatat
     1801 cgctggttat ccgggttgaa cccgagaagg agccaccagc gcatgccgtc gacgtagatg
     1861 ggccgtccgc tcccttccga tatgagccac cgcgccccct gcacctcttt attgttgaag
     1921 accggtttgt ccccggaagt ctcaagcgcc atcgacgtgg ggctgtcgcc cgtgacctgg
     1981 tagatgaggc cggtgttgaa gaggaggaag acgacgaaga agaccgagag cgcttgaaat
     2041 gccgtcctca tgagcggcag ggccgtgatc gcacggaccc tcccggcgac cgcctcatag
     2101 agcgctatgc cgccgatgat cgcgaacggg gcaaggagga tcagggtgat gtggtagagc
     2161 cggctggtgt tgagcgagct tgcgaagaag ggaacggcga tgccggctat gttgacgagg
     2221 aagaagacga tcgatatggc gagatactcg ggctcgatcc gccacctgcc gcgccgggca
     2281 agggttgcca gcagccccac ggcgatgagc ccctgggttg cgatatggac gacctttgcc
     2341 aggctgtgca ggggcgtgac ggactggctg gcgaggatat acataccctg gatggtcgcg
     2401 gggttgaaag gctccgcaaa aagggtgttt actatcttat ccccgatgcc ggtgatggtg
     2461 gcaaatgccg tgccttcggc taccgtcgag taccagaggt aggtgaggat agcaaggatg
     2521 aagatgtagg ggaggacgag cgtctggagg cgtggccggg gttcgtgcga ggcgagcggg
     2581 tcgtgtgcca gggtgcccgc catcccgcgg agcttctcca ggatgccgga cggcaggtag
     2641 tcgggaagga tcagcagcag ccatgccggt acgagcgaga agagatagat gtacgagagc
     2701 ccgtaatgcg agacgatcat ggcaaaaccg aaggtgatca tgaggaacgc ccgcctgctc
     2761 cggtccatgg atttgtcgac catcgcaagc acggtcagca cgaggaagag ttccgcgacc
     2821 tgctggcggg caagggagat catctcggta tagaacgtga agaacgacat gaagaagaag
     2881 accgagaaga acccgatctt ggggttcgtc tgcttctcga tcacccggta gagcgcgagc
     2941 ggcacgagcg cgaagaagag agggtagagg atcttgaaga cccagacgag atccaggtca
     3001 catatgagcg aatagatggg ggcgagcata acgaccgaga gcattccgtt tgtgttgtag
     3061 gggatggtca tatcccaggt ccctgatatc agcacgttgc ttgcgaggtg gagctcgtgg
     3121 tggatgtcgt acccccagat gtatcccgat atgagcgatg tgtggtagag gagcgcgagc
     3181 gcgaggctat agacggcaaa tgggtagcag gatgccggga tgaaccggtc gaacccgaca
     3241 acgaggccga tgacagcgat caggacgagg agcaggaaga ggagcgtgac catgtggtac
     3301 tcgttgcgta tgtacgtccc gacgatcgca aggaacggga gcagcgcgag aaacggcacg
     3361 gcgggcgggg ggatggagat ttccagccct gcatggctcg atccggcatg ttcccgatca
     3421 cgggtgagcg cgaggtagag caggatctgc acgacgccga cgatcgtcgg aaagaggacc
     3481 tcgagggata acggtcgtgc agaaccgagc agcgggtaga cggtgttggc gaagaggccg
     3541 gtgagcatga gaaccacgag gctcagtccg accgagtaga gcaccgtctc gatcgtgccg
     3601 agatcgtgga gcctgagcgc cttcagcacg aggatgcccg gcaggaaggt gaggtagacg
     3661 aaggccaggg cctcccgtgc gaccggtata tgatagccga tgaggtcgag gcagacgata
     3721 ccgatgaaag cgagctggat cgcctggaat gcccagaaga atcttctgtt ctcccagtcg
     3781 ttcatctgaa acgggtcggg caggcgcatt gtacctccgg gggatcagtc ggcgaccgat
     3841 tttgcggata tgggcgggat cttctcattc aggtctccat caggtctctg tatcgtgcac
     3901 tactatcggg cccgcctatt aaacctttgt gccggcggac cgctccccgg ggatatctcc
     3961 gcctgcggca ggggctgccg gcgctctgca acgcaccacc ggtcgagcag ggctgccgcc
     4021 tgcattcatc gctctttccc ggcggccggg gctcgcccgg tgaaggtcct gtctgcaggg
     4081 gtctcctctc tccccggagc gggcgcaagg tcgcggtaga ccggaaggat ggaccgtacg
     4141 atcgtgggcc atgccatatc cgtcttcagc ttcatgtagc cccgctctcc catgcgccgg
     4201 cacgcagccg ggtcctcaag cagtctcacg atcgcaccgg cgagggcctc cgggtcgcgt
     4261 ggaggcacga cgtacccggt ctctccgtcg tccacgatct ccggcaggct tcccgtatcg
     4321 gtgaccacca caggcctctt gaacccgtat gcggtcggta tgacgccgct ctgggatgcc
     4381 tcgatatacg ggagcgcgac gacgcttgcc tgctggaaga gccgggcgcc ctcttcatag
     4441 ggtatcctgt agttgtagac ctcgaataca tcccggtccg ccatcatctc ctcgtacctc
     4501 ccgaaatctt cgcctgtgcc ggctatgacg atccgggcgc cggggatctt ctgggtgatg
     4561 aggggttctg cctggatcag gcaatcgagc cccttgtaac ggtggatgcg gccgaaaaag
     4621 aggactctga ggccttcggg ctcccgcccc tcttcctcga acttcacaaa cggcgccacc
     4681 tcgtgctcgc ctatcgggat gacgtgcact ctctcttcgg gaacacggta gtttgccacc
     4741 aggatatcct tgagcgctct cccgtggaca aacatccggt cggtcagggc ccgcgacatg
     4801 aacagggtga gccggtagag cagggagccg atatcgagga ggcggtcttc gccggggtgc
     4861 ggttcgatgt cgtggaacgt cgccaccagg ggatacctcc tgagcatcgg tatgagcggg
     4921 cagagcatgg ggttgttcac ctgaaagtga acgatatcgg gcctgaagtc atggattgca
     4981 tcggcgatgt cccggatcac ggggtagcag gacgggccta accttcccgc gtatcgcgag
     5041 tacctgaaga tgcggatctt tacctccggg tcgatgctct ccaggtactc gtccgtcctg
     5101 taatcgggga ggagcagcag cacctcggtg tgccgggcga gctcgtttgc catctggatg
     5161 gtgtagtcgt agaaccagaa ggtcaggcat gccactcgca taggggcagt ctctgcggag
     5221 atctcttata taaatgttat gtctcccggg gccgttggtc ctgacggcgt gcgaccggca
     5281 gcggtgaatg gggggctccc ctcatccacg ggtgctctcc tgcacgctcc tgaaggctgg
     5341 cccgcatgcc cgacccgggt gtgccgggcc gtcggcgggc ggtagcggcc ggaggtcgcg
     5401 ggtcgtgccg gcgcacgagg caaaatctcc atcagcctta cgcaaatgcg tgcaggggat
     5461 ggtcgatctc ctgcaggcga tggcgaaagc gcgagtatta atacgttttg attttaattt
     5521 tggtaatgat ataataccct tttgcattat cctatattcc ctctcgaatg actttcaccc
     5581 gatgagcgaa ccccggaggg attatatggc taaatatccc ctttcacgag ttttattctt
     5641 tcaaaaaaat atttatagcg ttatcaaaag acggtagttc tacctcacag agaggctgaa
     5701 tggcatgata gacaggcgat atgtaccacg actcgttgca ctgctcctgg tgtgctgcct
     5761 tataccggca ttctctccgg taagcgcggc cgggtctgca actccggcca ccctcaccgt
     5821 ggctgcaaac gatagcaccg aactgtcaaa gacccaggca gactatgtct gtgatggagt
     5881 cgacgaccat gcggagatcc aggcagcgct cgccgcactg ccggaaggcg gcaaggtcgt
     5941 cctctcaaac ggcacgttca actgtgcggg cgtcatcgcc ccggcggggg ggacgacgct
     6001 ctccgggcag gggcccgatg cgacgaacct cgtcttcacc cgcgacggcc gcatcaccat
     6061 cgaccgtgag cacgtgaccc tggacgggtt cggcgtgaag ggcagcgg
//
LOCUS       DAOSEE010000123         6086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_4656_length_6086_cov_196.684132, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000123 DAOSEE010000000
VERSION     DAOSEE010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6086
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4656_length_6086_cov_196.684132"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PLI76_08875"
     CDS             <1..218
                     /locus_tag="PLI76_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOI62186.1"
                     /translation="KYSSLAYMITVIELSGAGKLVATKYFTYTESFAVVGIVYLALVS
                     ITTVAVNILEKQLAVPGTVRATAAVQL"
     gene            complement(257..1345)
                     /locus_tag="PLI76_08880"
     CDS             complement(257..1345)
                     /locus_tag="PLI76_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOI62187.1"
                     /translation="MESGSTLSHYRGPVLCALITAVLIGGSAPFTKLLLGGTGPLALA
                     ALVSLGSGAGALLFSLAGTRRSHMEAPPGRKEIPWLIGVTIFGGILAPVTLIQSLPGT
                     PAATAALLLNFEAVATTLIAAAVFRESVSRRVWIALGCITASCILLTRDPAAGFGLSL
                     PALGVLLTCLFWAVDNNMGQRLSAKDPLIIISVKGLVAGTITLIFALAAGEHLPDPAT
                     AAAAMAVGFLCYGGLTSILFLLALRGIGAARAGSLLAISPFFGVLFSLALFAELPAGA
                     FYAALPVMAAGAWLLVSEEHAHLHRHPPVVHEHRHRHDDLHHDHPHATGDPPLSPSGE
                     HSHLHEHGEIVHEHPHQPDIHHRHPHRR"
     gene            complement(1436..2209)
                     /locus_tag="PLI76_08885"
     CDS             complement(1436..2209)
                     /locus_tag="PLI76_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOI62188.1"
                     /translation="MRMHGILTVLACMVALLAVIGAGCTGTTDDQTYVVGIDGSYVPF
                     SYIDKDGNAQGFDVDSMRWIAEKKGINVKFEAVEWSAIIPTLQAGKIDMVYAGMSITP
                     ERLEKVNFSNPYWTVNQDVAARKDSNVTIEDVRAGKVVLGAQSGCTGAHWIEENVVGE
                     GLMPKENLKLYVDTQAAVSDLEIGRIDAVMYDDVSLREIIEGKPLKIIGSVQTQEQEQ
                     FGVAIRKDDTALLEFMNEALAELQADPYWEELKEKYNLK"
     gene            2533..2787
                     /locus_tag="PLI76_08890"
     CDS             2533..2787
                     /locus_tag="PLI76_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HOI62189.1"
                     /translation="MAGMKESATGMASGVAGTIQELDPQIIEVSTSGINYPASKSDLI
                     TRAKGAEAPQIVLDALDKFEDRQYKDPEDVKSEFQKIVKK"
     gene            2923..3606
                     /locus_tag="PLI76_08895"
     CDS             2923..3606
                     /locus_tag="PLI76_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOI62190.1"
                     /translation="MLIIGHRGARALAPENTLPALLKGMACADLVEIDVRLTGDEIPV
                     AIHDATVDRTTDGTGPVKGYTIEELKRLDAGEGEAIPTLREVLDLAHGQMGLIVEIKE
                     PGTEAIVVSVLMDRMPQRLILISFNPESVAMVKRLLPGVRAGLISTAEVDDPVGLARR
                     VQADLILPRWDRLSREVVERAHGAGLSVIPGTLNREDDLDEAVRLGVDGFATDDPCAA
                     RRYLEERRG"
     gene            complement(3619..3993)
                     /locus_tag="PLI76_08900"
     CDS             complement(3619..3993)
                     /locus_tag="PLI76_08900"
                     /inference="COORDINATES: protein motif:HMM:NF018767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HOI62191.1"
                     /translation="MRPAGRTVWAIPGGHIPLRTTGPEPEETSSDTVCLLNTGDRDAA
                     VEIRVYYEDREPVGPYRLTVPARRTRHIRFNDLIDPEPIPLDTDFAAVVISDLPIVVL
                     FARKDTSRAENAIAATLAFPCD"
     gene            complement(3990..5231)
                     /locus_tag="PLI76_08905"
     CDS             complement(3990..5231)
                     /locus_tag="PLI76_08905"
                     /inference="COORDINATES: protein motif:HMM:NF015963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HOI62192.1"
                     /translation="MAPQAPARDARLTPGTARMWENWSGSLRFSPERVERPESESAIR
                     DIVRRAAGAKKTLRPAGTGHSSSLLLATRDVLISLDAFRGIESYDPAKREATLLAGTT
                     LGEAGNLLLALELAMHNLGDVDVQSVAGALATGTHGSGKHLPALSAMVAGGRMITASG
                     EKVEFSLEKDPDLTRAAQVSLGALGIYTALTLRLLPAYRLHRREWCTTADRCFAHIDD
                     LAERHRNFDFYWYPRSDRVKLRTMDPPGDAPESIPYARLVEDRTGWSSEVIAKVRTLR
                     FDEMEYAVPAEVGPECFEAVRRRIRERHRKHVAWRVLYRTVAPDDAYLSIAHGRATAT
                     ISLHQNATLPFWGFFNDIEPIFQEYGGRPHWAKKHTMAADDLMPLYPRWDRFIAARRR
                     MDPDGLFLNPYLERLLGEEKA"
     gene            complement(5195..5893)
                     /locus_tag="PLI76_08910"
     CDS             complement(5195..5893)
                     /locus_tag="PLI76_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOI62193.1"
                     /translation="MLGTATIDVDATRIEVRPTDVRRIPQAGEAYDVVEIETDRGTTT
                     CRYYEAEGARAGVVMVGGVGGGFDTPGRGLYPRLAEGLQNDRIGALRVRYRHPTNLVE
                     SVVDTAIGMRYLESRGIRAIGLVGHSLGGAVVIQAAAGDPLVRVVVALSTQDAGTGPV
                     ARLAGRAAVLLIHGDADTVISPRSSEEVYRRTHEPKRLVIYEGAGHALDEAAESLAVE
                     VKAWLLKHLPGTLV"
BASE COUNT         1015 a         1909 c         2089 g         1073 t
ORIGIN      
        1 tcaagtactc gtcgcttgcc tacatgatca ccgtgatcga gctctccggg gcgggaaagc
       61 tggtggcgac gaagtacttc acctacacgg agtcgtttgc agtcgtcggc atcgtctacc
      121 tcgcgctggt ctcgataacg accgtcgcgg tcaacatcct cgagaaacag ctggccgttc
      181 ccgggacggt ccgggctacc gccgcggtcc agctctagga catccggctg ccaccctctt
      241 tttgcaggtg tccgcgttat cgccggtgcg ggtggcggtg atggatgtcg ggctggtgcg
      301 gatgctcgtg gacgatctcg ccgtgttcgt gcaggtggga gtgctcaccg gacggggaga
      361 gcggcgggtc gccggttgcg tgggggtggt cgtggtgaag gtcgtcgtgg cggtgccggt
      421 gctcgtggac gaccggcggg tgccggtgga ggtgggcatg ctcctcggag accagcagcc
      481 atgcgcctgc ggccatgacg ggcagcgcgg cgtagaacgc gcccgccggg agttctgcaa
      541 agagggcgag ggagaagagg acgccaaaga acggggatat ggcaaggagc gagcccgccc
      601 gggcggcgcc gatgccccgg agggcgagga ggaagaggat gctcgtcaga cccccgtagc
      661 agaggaaccc gacggccatg gcggcggctg ccgtggcagg gtccgggagg tgttctccgg
      721 cggcgagagc gaagatgagc gtgatggtgc cggcaacgag cccttttacg gatatgatga
      781 tgaggggatc ctttgccgag agccgctgcc ccatgttgtt gtcgaccgcc cagaagaggc
      841 aggtgagcag gacaccgagc gcggggaggg agaggccgaa gcccgccgcc gggtcgcggg
      901 tgaggaggat gcaggatgcg gtgatgcacc cgagcgcaat ccagacgcgg cggctcaccg
      961 actcccgaaa gaccgccgcg gctatcagcg tcgtcgcgac ggcctcgaag ttcaggagga
     1021 gggcggccgt cgccgccggc gtccccggga ggctctgtat cagggtgacc ggcgcgagga
     1081 ttccgccgaa tatcgtcacc ccgatcagcc aggggatctc cttcctcccg ggcggcgcct
     1141 ccatgtggct ccgccgggtc cccgcgaggg agaagagcag ggcgccggcg ccgctcccga
     1201 gcgagaccag cgcagcgagc gcgagcggcc cggtcccgcc gaggaggagt ttggtgaacg
     1261 gggcgctccc gccgatcagg acggccgtta tcagcgcaca gaggacgggg ccccggtagt
     1321 gcgagagagt ggatccgctc tctatggcgg cgggtttgcg cgggtacaga agagaggcct
     1381 ccgggagttt gaagatctaa aagagggagg ggacgatcgg tccccatagt ccccgttact
     1441 tcaggttgta tttctctttg agctcttccc agtagggatc ggcctgcagc tctgcaagcg
     1501 cctcgttcat gaactcaagg agggcggtgt catccttcct gatggcgacg ccgaactgct
     1561 cctgctcctg cgtctgaaca gacccgatga tcttcagcgg cttgccttcg atgatctccc
     1621 tgagcgagac atcgtcgtac atgaccgcat cgatcctgcc gatctcaagg tcgctgacgg
     1681 cggcctgggt atcgacgtag agtttcaggt tctccttcgg catcagccct tctccgacga
     1741 cgttctcctc gatccagtgg gcgccggtgc atccgctctg cgcaccgaga accaccttgc
     1801 cggcacggac atcctcgatc gttacgttcg agtccttacg agccgcgaca tcctggttga
     1861 ccgtccagta ggggttgctg aagttgacct tctccagccg ctcgggggtg atcgacatcc
     1921 ccgcgtagac catatcgatc ttcccggcct ggagggtggg gatgatggcg ctccactcta
     1981 ccgcctcgaa cttcacgttg atgcccttct tctcggcgat ccatctcatg gagtcgacgt
     2041 cgaacccctg ggcattgccg tccttgtcga tgtagctgaa cggcacgtac gaaccgtcga
     2101 tgccgacgac gtaggtctgg tcgtcggtcg tgccggtgca tcctgccccg atgactgcca
     2161 gtagtgcgac catacaggca agcactgtaa gaataccatg cattctcata gagaaggggt
     2221 tagtcaggta agtaagaaga accttctgat ctggcccggg gaagagccgg tttgccccat
     2281 cccggagcca tgccgttccc ggtgcatgtt ccggccgccg tcccctggtt cggcgcaccg
     2341 gacaggcgat catctctcca aggctcacga tcggccgtga atccccgggt agccttccaa
     2401 tgtcgcaggt tcggctgtat ccggccgata cagggcaccc tgcccgccgc cgtacgggcg
     2461 ataggtattt attgcatctt acccactggg ggtcgcctcc cggaaggggg cacagatacg
     2521 ctggtgagat atatggcagg tatgaaggaa tccgctacgg gcatggcgtc aggtgtcgcc
     2581 gggaccatac aggagctcga tccgcagatc atcgaggtga gcacttcggg gataaactat
     2641 cctgcatcga agagcgacct gatcacacgg gcaaaggggg ccgaggcacc ccagatcgtc
     2701 ctcgatgcgc tcgacaagtt cgaggaccgg cagtacaaag atccggaaga tgtgaagtcc
     2761 gagttccaga agatcgtgaa gaagtgaact tttctggccg caggcgccag gatgtggcgc
     2821 ctgccggctc ccatttccga ctgtgcaatc tggcggtctc tcgagagagc cgtgcaacgg
     2881 agacccgcca tctatatcta tcccccgggt agacggcaca ctatgctcat catcggacac
     2941 cgcggcgccc gggctcttgc accggaaaac actctcccgg ccctcttgaa gggtatggca
     3001 tgcgccgatc tcgtcgagat cgacgtccgc ctcacggggg acgagatccc cgtcgcaatc
     3061 catgacgcga ccgtcgaccg gacgacggat gggaccgggc cggtcaaggg ctacaccatt
     3121 gaagagctga agaggctcga tgcgggcgag ggtgaggcga taccgacgct ccgtgaggtc
     3181 ctcgacctcg cccacgggca gatggggctc atcgtcgaga tcaaggagcc cgggaccgag
     3241 gcgatcgtcg tctcggtgct catggaccgg atgccgcaga ggctcatcct gatctcgttt
     3301 aacccggaga gcgtcgcgat ggtaaaaagg ctcctccccg gcgtgcgggc gggcctcata
     3361 tccactgcgg aggtcgacga tcccgtcggg cttgcccgcc gggtgcaggc ggaccttatc
     3421 ctcccgcgat gggaccgcct ctcgcgcgag gtggtggaac gggcgcacgg ggccgggtta
     3481 tctgtcatac cgggaacgct gaatagggag gacgacctcg atgaggccgt ccggctcggc
     3541 gtcgacggtt ttgcgaccga cgatccttgc gccgcacgcc gctacctgga ggagaggaga
     3601 gggtgatccg ccggccgatc agtcgcacgg gaacgcgagc gtggctgcga tagcgttctc
     3661 ggcccggctg gtgtctttcc gggcaaagag cacgacgata ggaagatccg atatgacgac
     3721 agcggcaaaa tcggtatcca gcggtatggg ttcggggtcg atcaggtcgt tgaagcggat
     3781 atgccgggtc cgccgggccg gcaccgtgag gcgataaggg ccgacaggct cgcggtcttc
     3841 gtagtagacc ctgatctcga ccgccgcatc ccggtccccg gtgttgagca ggcagaccgt
     3901 gtcgctgctg gtctcctccg gctccgggcc ggtcgtccgg agggggatat gtccgccggg
     3961 tatcgcccag accgtcctcc ctgccggcct catgccttct cctccccgag cagcctctcc
     4021 aggtaggggt tgaggaagag gccgtccggg tccatgcggc ggcgggcggc aataaaccgg
     4081 tcccaccggg gatagagcgg catgaggtcg tcggccgcca tcgtgtgttt cttcgcccag
     4141 tgcggccgcc ccccgtactc ctggaatatc ggctcgatat cgttgaagaa gccccagaaa
     4201 ggcagcgtcg cgttctggtg gagcgatatc gtggcggtcg cgcggccgtg tgcaatgctc
     4261 aggtaggcgt cgtccggcgc gaccgtccgg tagaggaccc tccaggccac gtgttttctg
     4321 tggcgttccc gtatgcgccg gcggaccgcc tcgaagcatt cgggcccaac ctccgccggg
     4381 acggcatact ccatctcgtc gaaccggagg gtgcggacct ttgcgatgac ctcgctgctc
     4441 cacccggtcc tgtcctcgac gaggcgggca taggggatgc tctccggcgc atctccggga
     4501 ggatccatcg tcctgagttt cacccggtcg ctcctggggt accagtagaa gtcgaagttc
     4561 cggtgcctct cggcgaggtc gtcgatgtgg gcgaagcagc ggtcggcggt ggtgcaccac
     4621 tcccggcggt ggagccggta tgccgggagc aggcggagcg tcagggccgt gtagatgccg
     4681 agcgctccga gcgagacctg tgccgcccgg gtgaggtccg ggtccttctc aagagagaac
     4741 tccaccttct cgcccgatgc cgtgatcatc cgcccgccgg cgaccatggc ggagagggcc
     4801 ggcaggtgct ttccgctgcc gtgcgtcccc gtcgccaggg cgcccgcgac gctctggacg
     4861 tcgacgtcgc cgaggttatg catggcaagc tcgagggcga ggaggaggtt cccggcctct
     4921 ccgagcgtcg tgccggctaa aagggttgcc tcgcgctttg cagggtcata cgactcgatg
     4981 cccctgaagg catcgagcga gatgagtaca tcccgggtcg cgaggaggag cgacgacgag
     5041 tggccggtcc cggcggggcg gagcgtcttc ttcgctcctg ccgcccggcg tacgatgtcg
     5101 cggatcgcgc tctcgctctc cggccgttct acccgttccg gtgaaaaacg gaggctcccc
     5161 gaccagttct cccacatgcg tgccgtcccc ggggtcagac gagcgtcccg ggcaggtgct
     5221 tgaggagcca cgccttcacc tcgacggcga ggctctccgc cgcctcgtcg agggcgtggc
     5281 ccgccccctc gtagatgacc agccgtttgg gctcgtgggt gcgccggtag acctcctccg
     5341 acgaccgggg agatatgacc gtgtcggcat ccccgtggat caggaggacc gccgcccggc
     5401 ccgcaaggcg ggcgaccggt ccggtcccgg cgtcctgggt cgagagggcg acgacgaccc
     5461 ggaccagggg gtcgcctgcc gccgcctgga tcaccaccgc tcccccgagg gagtgcccga
     5521 cgagcccgat ggccctgatg ccccggctct ccaggtagcg cataccgatg gcggtatcga
     5581 cgaccgactc cacgaggttg gtcgggtgcc ggtaccgcac ccggagtgcg ccgatcctgt
     5641 cgttctggag gccttctgcc aggcgcgggt agagcccccg ccccggggtg tcaaacccgc
     5701 cgccgacacc acccaccatg acgacgccgg cgcgtgcccc ctccgcctcg tagtagcggc
     5761 aggtggtcgt cccccggtcg gtctcgatct ccacgacatc gtaggcctcg ccggcctggg
     5821 ggatcctcct tacatccgtg gggcgaacct caatccgcgt agcatccaca tcaatcgtag
     5881 ccgtcccgag catggaggag agtggatacg gtgctatttg tcctttgtgg ctgccgcttc
     5941 catgcggtct aaccgttgca gatcccgggc ggggttttgt atgggcaagc cccgggcggg
     6001 gatggcggga agaaatccgg gttcccgccg gcggagagac aggcagggtc ggcgcgatgt
     6061 gcggggtcct gagggcgtga gagaca
//
LOCUS       DAOSEE010000124         6047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_4692_length_6047_cov_238.541722, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000124 DAOSEE010000000
VERSION     DAOSEE010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6047
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4692_length_6047_cov_238.541722"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..1436
                     /locus_tag="PLI76_08915"
     CDS             138..1436
                     /locus_tag="PLI76_08915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOI62194.1"
                     /translation="MHKSDRAYAGLIGMSAIHGIRAYERYAGRETIRRIEAKARPLRD
                     MHILHMNSTYYGGGVSQILSSLTLLMNGLGIRTGWRVVHGPPDFFSVTKKFHNALQGA
                     DINLTDRKKEIYEGVIRENAMRNHLDHDVVFIHDPQPLPMITHYRKRSPWIWRCHVDL
                     TTPNQKVWNYFAPLIEKYDAVILSCKEYRQDLRTPQIFFMPAIDPFSIVNRELSESAI
                     DERLAYYNIPTDLPLVVQISRFDRWKDPLGVIQAFEKARKEVECTLVLVGNVATDDPE
                     GAEVYRSLLAHRNERVIILSVQDGALVNALQRRAAVVLQKSIREGFGLTVSEAMWKGA
                     AVIGGNVGGILHQIRDGENGFLVSSVDEAAGRIVQLLRDPDLARRLGHAAHETVRERF
                     LFTRTIEQYLDLIGSFEPEFRLKGMKEPSCEQPANPDRTG"
     gene            1663..2412
                     /locus_tag="PLI76_08920"
     CDS             1663..2412
                     /locus_tag="PLI76_08920"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HOI62195.1"
                     /translation="MAIQVIGVSGLPLIQKGDDLPALICGRVTLEDGDILTIASSVYS
                     KAKGFTRELSAITPGADAVRIAEKTKEDPRFVQAVLDSSTDILLEYPFILSEVPSGHI
                     GVRAGVDHSNIEDGRIIVLPPDPMAAAAEIRDAIRRITGKDVRVIITDTCGRSFRRGQ
                     TGIAIGWAGMTAINDYRGDTDLFGHILQITEEAVVDEIAAFANFVMGESHQGVPAVVF
                     RNCKAWKGHDAVYFTPEEDIIRAALAKNKKD"
     gene            complement(2409..2567)
                     /locus_tag="PLI76_08925"
     CDS             complement(2409..2567)
                     /locus_tag="PLI76_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit Sec61beta"
                     /protein_id="HOI62196.1"
                     /translation="MAKKSGGRLVSSAGLVNYYDSEDHRAIHISPVAVIIVTAVIGVA
                     VFILNALF"
     gene            2612..3658
                     /locus_tag="PLI76_08930"
     CDS             2612..3658
                     /locus_tag="PLI76_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOI62197.1"
                     /translation="MQQVAPYTITGRALLGEDLEEEDVAITVSGGIVTAIEPYRGTPE
                     RWIVPAFFNAHTHLGDTVAMDLPARGSLAELVKPPDGLKHRILAATPRAELVRGMRSS
                     IKTMIATGTAGFADFREGGAAGVAAIREAAAGLDCRPVILGREGGERVSDGAGISSAH
                     DVKNAEEVVREARKAGKLVAFHAGEKNPDDIDDALAFDPDLLVHCTHATDAHLRRIVD
                     MGIPIAVCPRSNWLLGVAASPARPPIARILELGGRLLLGTDNVMFVQPDMLREMAFTA
                     TVYRAPPKEILRAAIAGATLAGRTGYLEIGQNTKMLVINPAKCNLSFSKDIRTTIVKR
                     LDPSSIEQNVLTLQ"
     gene            3695..4123
                     /locus_tag="PLI76_08935"
     CDS             3695..4123
                     /locus_tag="PLI76_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOI62198.1"
                     /translation="MFRKILVAIDGSEPASHALDAALDEARVRNAEVHVVYVIESGLF
                     SSLPMDNTLEIIYSVLQKEGEEILESAKKKADAKNIPLTTHLLQGHAGSRIASLAEEL
                     GVDLIVLGSYGKSGVDRLLLGSVTDYVVQNSPVTTLVVRS"
     gene            4138..4998
                     /locus_tag="PLI76_08940"
     CDS             4138..4998
                     /locus_tag="PLI76_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOI62199.1"
                     /translation="MLVRDFMVTDVVCVEIPGNRDDVLRILKRTGISGVPVLKGGELV
                     GIITRKDLLRKAEETQLGLLMTPDPVVIRPDAPLPEAAQLMVRHNIRRLPVVENGSMV
                     GIISVADLIGAIAQMRLAVPIRDNYISKTYALWEETPLSLVGRVLEISGYDAIPILME
                     DGTLTGIISERDLIRHTRIEDGVEVSDFSNGTDDDEWTWESIRDMHTISYGISRIQLL
                     PIPVKNAMVRNVLAVPINAEVGECALKMRRARVDQLPVVNGNKRLIAMLFDRELIKAL
                     LPDQPGLRKN"
     gene            5144..5788
                     /gene="psmB"
                     /locus_tag="PLI76_08945"
     CDS             5144..5788
                     /gene="psmB"
                     /locus_tag="PLI76_08945"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit beta"
                     /protein_id="HOI62200.1"
                     /translation="MLDTSQEIMKGTTTVGLIFNEGVVLATEMRATMGNMIASKRAKK
                     IYQITPRIGMTTAGGVGDAQQLVRILQVECNLFEMRRGKTMSVGAASTLLSNYLNQNR
                     YYPYYVQLLMGGFDDEGPSVYSVDALGGATKEEEIVATGSGSPFAYGVLEDQYRPDMK
                     EDEAKDLAVRAVKSAMRRDSASGEDIMVVVITKDKYEEHIERGLQRPSVARSIT"
     gene            5809..>6047
                     /locus_tag="PLI76_08950"
     CDS             5809..>6047
                     /locus_tag="PLI76_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-CASP ribonuclease aCPSF1"
                     /protein_id="HOI62201.1"
                     /translation="MVIEEKLRELKEQINKIVPGGITISDVEFEGPELVIYTDDPKQF
                     ADQADLIKVLARDLRKRIVVRPNILEDPERAAQEIR"
BASE COUNT         1294 a         1848 c         1827 g         1078 t
ORIGIN      
        1 gggggattgc cggcgaactg cgtatgcgtg gccaggcggg gtggaatccg cgttacgggg
       61 atgaccagcg gggaacaccc gggtccggca cccctcctcg ggggtgaggt ccccgatcta
      121 cgcgggggtt cgccggtatg cataagagcg atcgcgcgta tgcagggctg atcgggatga
      181 gcgcaattca cggtatcagg gcatatgagc ggtatgcagg aagagagacg atacggcgta
      241 tcgaggcgaa agcccgcccg ctccgggata tgcatatact gcacatgaac tccacctact
      301 acggcggcgg cgtctcgcag atcctctcct ccctgacgct cctgatgaac ggcctcggca
      361 tacggaccgg gtggcgcgtc gtgcacggcc cgccggactt cttcagcgtg acaaagaagt
      421 tccataacgc ccttcaggga gcggatatca acctgaccga ccggaagaag gagatctacg
      481 agggggtcat ccgcgagaac gccatgcgga accacctcga tcacgacgtg gtcttcatcc
      541 acgatccgca acccctcccg atgatcaccc attaccggaa gaggagcccc tggatatggc
      601 gctgccatgt cgacctcacc acaccgaacc agaaggtctg gaactacttt gccccgctca
      661 tcgagaagta cgacgccgtc atcctctcct gcaaagagta ccgccaggat ctcaggacgc
      721 cgcagatctt cttcatgccc gccatcgacc ccttctccat agtgaaccgg gagctctccg
      781 agagcgcaat cgacgagcgt cttgcgtact acaatatccc gaccgacctc cccctggtcg
      841 tgcagatctc ccggttcgac cgctggaaag acccgctggg ggtcatccag gcgtttgaga
      901 aagcgaggaa agaagtggag tgcaccctgg tcctcgtcgg aaacgtggcg accgatgatc
      961 cggagggcgc tgaggtctac cggtcactcc ttgcccaccg gaatgaacgg gtcatcatcc
     1021 tgagcgtcca ggatggggcg ctcgtgaacg cgctccagcg ccgcgcagcg gtcgtcctgc
     1081 agaagtccat ccgggaaggg ttcgggctga cggtatcgga ggcgatgtgg aagggggcgg
     1141 cggtgatcgg cgggaacgtg ggcggtatcc tccaccagat acgggatgga gagaacgggt
     1201 ttctggtctc ctccgtcgat gaagcggccg ggcggatcgt ccagctgctc cgcgatcccg
     1261 acctggcgcg ccggctcggg catgcggcgc acgagacggt gcgggagcgg ttcctcttca
     1321 cccgcaccat cgagcagtac ctcgacctga tcggctcgtt tgagccggag ttccggctga
     1381 aggggatgaa ggagccatcc tgcgagcaac ctgcaaaccc ggacagaaca gggtgagaac
     1441 cctgatggga ggaaggatat ttggcagaga tcccgcgtac atcaacggtg caccggcaga
     1501 tggaaagagg gattgagaac acccccatgg tatcgcgcgc actgcgggat cgcgcaccgt
     1561 agccctcgtc agggccgcta tcggcaaaat ccgccggtat ccttccatag taaacccgag
     1621 catttttctg cgagcgggtg cgatcactga tctgtgagtt ccatggctat ccaggtaatc
     1681 ggtgtttcag gcctccccct gatccagaaa ggcgacgacc tgcccgccct catctgcggc
     1741 cgggtcacgc ttgaagatgg agatatcctt acaattgctt catcggtcta ctcaaaggca
     1801 aaggggttca cccgtgaact ctccgccatc actcccggcg cggatgccgt gcggatcgcg
     1861 gagaagacaa aggaagaccc ccgattcgtg caggccgtgc tggactcttc caccgatatt
     1921 ctgctcgagt acccgttcat cctctccgag gtgccgtccg gccacatcgg cgtccgggca
     1981 ggcgtcgatc acagcaacat cgaggacggc cggatcatcg ttctcccgcc cgaccccatg
     2041 gcggccgccg ccgagatccg cgacgccatc cgcaggatca cgggaaaaga cgtccgggtg
     2101 atcatcaccg acacctgcgg caggtcgttt cggcgcggcc agaccggcat cgccatcggg
     2161 tgggcgggta tgacggcgat taacgactac cggggcgaca ccgacctctt cggccacatc
     2221 ctccagatca ccgaagaagc ggtggtcgat gagattgcgg cattcgcgaa cttcgtcatg
     2281 ggcgagagcc accagggagt ccccgccgtg gtcttccgga actgcaaggc ctggaaaggg
     2341 cacgacgccg tctacttcac gcccgaagaa gatatcatca gggccgccct cgcaaagaac
     2401 aaaaaggatt agaagagggc attgaggata aagactgcca ctccgatcac cgcggtcacg
     2461 atgatcacgg ccaccgggct gatatggatg gcccggtggt cctcgctgtc gtagtagttg
     2521 accaggccgg cagaggatac cagtcttccg ccagattttt tcgccataca gaaatatact
     2581 cctttgccat atataaaaca aaggtcgaac gatgcagcaa gtcgcaccgt ataccatcac
     2641 cggccgggcg ctcctcggcg aagacctcga ggaggaagac gtcgccatca ccgtctcggg
     2701 gggtatcgtc accgcgatag aaccttaccg gggaacgccg gaacggtgga tcgtgcccgc
     2761 cttcttcaac gcccacaccc acctcggcga caccgtcgcg atggatctcc ccgcccgggg
     2821 cagccttgcc gaactcgtca aacccccgga cggcctgaaa caccggatcc tcgcggcaac
     2881 cccgcgggcc gaactcgtca ggggaatgcg gtcgagcatc aagacgatga tcgcgaccgg
     2941 aacggccggg tttgccgact tccgggaagg gggggccgcc ggtgtcgcgg cgatccgcga
     3001 ggctgcggcc gggctcgact gccgcccggt cattctcggc cgcgaaggcg gggagcgggt
     3061 gagcgacggg gcgggcatca gcagcgccca cgatgtgaag aacgccgaag aggtcgtcag
     3121 ggaggccaga aaggccggca aactcgtcgc ctttcatgcc ggggagaaga acccggacga
     3181 catcgacgat gcgctcgcgt tcgatcccga cctgctcgtc cactgcaccc acgcgacgga
     3241 tgcgcacctc cgccggatcg tcgatatggg gatcccgatc gccgtctgcc cgcgatcgaa
     3301 ctggctcctc ggggttgcag cgtcgccggc ccgcccacct atagcgcgca tactcgaact
     3361 cggtggccgg ctcctcctcg ggacggataa cgtaatgttc gtccagcccg acatgctccg
     3421 ggagatggct tttaccgcaa ccgtctaccg cgcgccccca aaagagatcc tgcgtgccgc
     3481 gatcgcgggc gcaacgctcg ccggcaggac cggatacctg gagatcgggc agaataccaa
     3541 aatgctcgtc ataaacccgg caaaatgcaa cctctccttc agtaaagata tccggacgac
     3601 cattgtgaag cgactggatc catcctccat cgaacaaaat gttttaaccc tgcagtagaa
     3661 atctgataag aagtatctgt tctgcgtggt gtgtatgttc cgtaagatac tcgttgctat
     3721 agatggatcc gaaccggcca gtcatgcgct ggatgcggcc ctcgatgagg cccgtgtccg
     3781 gaatgccgag gttcatgtcg tgtatgtgat cgaatccggg ctgttttcat cccttcccat
     3841 ggacaacaca ctcgagataa tctacagtgt cctgcagaag gaaggcgagg agatcctcga
     3901 atccgccaaa aagaaggcag atgcaaagaa tatcccactc accacgcacc tcctgcaggg
     3961 gcatgcggga tcgcggatcg cttccctcgc cgaagagtta ggagtcgatc tcatcgtcct
     4021 cggatcttac ggcaagagcg gcgtcgatcg ccttctcctc gggagcgtga cagactatgt
     4081 cgtccagaac agccccgtca cgaccctggt ggtgagatca taacatcctc gcgacagatg
     4141 cttgtccgtg acttcatggt gaccgacgtc gtctgcgtcg agattcccgg gaacagagac
     4201 gatgtcctgc gtatactgaa acgcacgggc atcagcggcg tgccggtctt aaagggcggc
     4261 gagctcgtcg ggatcatcac ccggaaagat ctcctccgca aggcggaaga gacccagctc
     4321 ggacttttga tgacgcccga cccggtcgtc atcaggccgg atgcccccct ccctgaagcg
     4381 gcgcagttga tggtccggca caacatccgg aggctcccgg tcgtggagaa cgggtccatg
     4441 gtcggtatca tcagcgtcgc cgacctcatc ggcgccatcg cccagatgcg cctggcggtg
     4501 ccgatccggg acaactacat cagcaagacc tacgcactct gggaggagac gcccctctcg
     4561 ctcgtcgggc gagttcttga gatatcgggc tacgatgcca tccctatcct gatggaagac
     4621 ggcacgctca ccggcatcat atcggagcgc gacctcatcc ggcacacccg catcgaggac
     4681 ggcgtcgagg tcagcgactt ctcgaacggc acggacgacg acgagtggac ctgggagagc
     4741 atcagggata tgcacaccat cagctacggg atctcgcgga tccagctcct ccccatcccc
     4801 gtcaagaacg ctatggtccg aaacgtcctt gccgtcccca tcaacgccga ggtcggcgaa
     4861 tgcgcactga agatgcggcg ggcccgggtc gaccagctcc cggtggtcaa cgggaacaaa
     4921 agactcatcg ccatgctctt cgaccgggag ctgatcaagg ccctgctgcc ggaccagccg
     4981 ggcttgcgaa agaattaagg ggcaacatta tataacatag tgatgcttat cgtatcgtgc
     5041 tgaaacccgg tacgacgata aatttatgtt ttatgatatc gctgtatacg tttgaccgct
     5101 tttgagcgat tcaaacgtct ttaaaggttt tagaggtgct tcaatgcttg atacttccca
     5161 ggaaattatg aagggtacaa ccacggtggg actgatcttc aacgagggcg tggttcttgc
     5221 caccgagatg cgggcgacga tgggcaacat gatcgcgagc aagcgtgcca agaagatcta
     5281 ccagatcacg ccgcggatcg gcatgaccac cgccggcggc gtcggcgacg cgcagcaact
     5341 cgtccggatc ctccaggtcg agtgcaacct ctttgagatg cgccgcggca agaccatgtc
     5401 ggtcggcgcg gcatcgaccc tgctctcgaa ctacttgaac cagaaccggt attatcccta
     5461 ctacgtccag ctcctgatgg gcgggttcga cgacgagggg ccgagcgtct actccgtcga
     5521 tgcgctgggc ggggcgacca aggaagagga gatcgtcgcc acggggtcgg ggtctccctt
     5581 tgcctacggc gtgctcgagg accagtaccg tccggacatg aaggaagacg aggcgaagga
     5641 cctcgccgtc cgcgccgtca agtcggcgat gcgccgcgac tccgcatccg gtgaggatat
     5701 catggtggtc gtgataacga aagataaata cgaagaacat atcgagcgcg ggttgcagcg
     5761 accatccgtg gcccgctcga taacctgata ttttttagga cgatgtgaat ggtgattgaa
     5821 gagaaacttc gggagctgaa ggagcagatc aataagatcg ttcccggcgg gattacgatc
     5881 tcagacgtcg agttcgaagg gcctgagctc gtcatctaca ctgatgaccc aaaacagttt
     5941 gccgaccagg cggacctgat caaggtcctt gcgcgcgatc tgcgcaagcg catcgtcgtg
     6001 cgcccgaaca tcctcgaaga tccggaacgg gcggcccagg agatccg
//
LOCUS       DAOSEE010000125         5924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_4816_length_5924_cov_242.180099, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000125 DAOSEE010000000
VERSION     DAOSEE010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5924
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4816_length_5924_cov_242.180099"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1518)
                     /locus_tag="PLI76_08955"
     CDS             complement(<1..1518)
                     /locus_tag="PLI76_08955"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HOI62202.1"
                     /translation="MRRESILAFILLTCALATVAGAAAVDLSVVWNKTYGKPGANDSA
                     YSIIQIPGDRGYVFSGETESFGAGEADAWVVDLSRSGDERWNRTYGDRGMDTARSVIN
                     TSDGNYLFVGAFTYIMGEDRKDTDVLVVKFSPTGDIIWNETFGGPEDNATAYAVAETA
                     DRGYIVAGKTAFWGEPDTDALVIKLNRSGSEEWSRTFGEPGLNDTAYAVVAAPSGDIG
                     IAGSTESFGAFAADAFVQKIDSSGNETWNVTFGGAENDTARSIVLAPDGGFVFAGSYM
                     SRTASGRTDDDALVVRMRPDGKVAWNWTYGNADENESAESIIATRDGGYLFAGRTGES
                     PLDNDAWIVKLDGEGAVEGDLTIGGRNPGDRAASVIQTTASEYVFAGTFNSTKKGGTP
                     DTDAWVVKLAAKPKTIIKPPPKPPKPPNVCPGQCPPPTPKPPVTQKPQTGSIGDLVWN
                     DTNKNGIQDKGENGIKGVGVELLGSDGKTVVKQTKTDSRGTYLFSGLQPGEYYIEF"
     gene            complement(1825..2424)
                     /locus_tag="PLI76_08960"
     CDS             complement(1825..2424)
                     /locus_tag="PLI76_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HOI62203.1"
                     /translation="MLGTVPGIDPEVLSLVIVPAFIFFARICDVTIGTMRIIFVSRGM
                     KMVAPVLGFFEVFIWIIAVGQIFQNLTNPLNYFAYAAGFATGNYVGMLVEERLAMGLA
                     LIRVITQRDATNLIDYLRGAGYGVTVLDAQGKQGPGKVIFSVIKRKNIKDVEKAIHEF
                     NPKAFYSIEDVRRAAEGTFPTAVPGLTPLHWHRTTRKGK"
     gene            3106..>5924
                     /locus_tag="PLI76_08965"
     CDS             3106..>5924
                     /locus_tag="PLI76_08965"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOI62204.1"
                     /translation="MLIAPAAADSLPDRVYVNASYNDETPDYGKLNFSSIQEAVDNVT
                     DGGEVWVYNGTYNEPIVIDRAMTVTTVSHLECPGLDGVIIDAMGAQAGIEIDADEVSI
                     SGFEVVNASGVGIYAYNAGAVTIEYNSVTVENDTYELTCGILIEGGEGVCIGNNAVRV
                     IGSSAQAGIMVNGVTEANVHDNHVVAVSRHQGLEAVEMEPNPVVAALFTEADEPFIAA
                     RATVTQQASAGVIIEESSSVLVERNRIVAVGNCTEDALDLHYSAVEAWGIRSGGNSKN
                     LKILENDVTVVGKALDTAWTVGILGVGDLALVQSNTVEVIARSRVVLPVGIVISEAEK
                     GQVLDNEIEVEAACIGKSDGEVSPEATGIIAVESPKAQVLRNDVLYTLILTDAGQLAM
                     TSTEGIYIGGCDGSRVSENIVLIQTGIFNLPVDGDESTEDEEPLTLGVARVKGIGVEA
                     SDKPEICDNVVHAFGAVMTYDETAGDLNVGALSALVMTGIHVWSYSDDLLENPVVREN
                     VVVVRAQVQTNLVPELELDEEEVKAALAGNSALLARYVGMMQESEEFSVATCLADTKG
                     LSALTESDLEFEPDIDEATLAAALTVTAGIVLEDVLNPEVCCNYVPVYQDVAVIVFDR
                     PVDDVGMVLQTPLSLGLIDQAFSDEQVQQAFLLGLIDEDESAWQNLSKDDRAAITQAV
                     LAGDTGVLAAYTDDETLAGYQALMEDGEAFEFLNTTRLAAAIPLSVSCGVYVNDAEGD
                     VVLHDNEIVVETNAAGLAAAQGGDLDAPDAAAGSAGIVAAFGFAGTGDSISVEENCIH
                     VITNGMFLGQAMGGENETADTAAGTANLFLGVGVLADADGEHVVNNDITVAQRSRAQT
                     LAFNIVKDRTALSVAATAGVGIGIVLDPYDVFDPETYEPAPPAPDEWFASVIEGNTIA
                     VTNDINTFSLAEMLLPAPLDGSSNAL"
BASE COUNT         1196 a         1809 c         1730 g         1189 t
ORIGIN      
        1 gaactcgatg taatactcgc cgggctgcag accgctgaag aggtacgtgc cccgggaatc
       61 ggtttttgtc tgctttacca cagtcttgcc gtcggatccg agcagttcca caccgacgcc
      121 cttgatgccg ttctcaccct tatcctggat gccgttcttg ttcgtatcgt tccagacgag
      181 atcgccgata ctgccggtct gcggtttttg ggttaccggc ggcttcggcg tgggaggcgg
      241 acactgcccg ggacagacat tcggcggttt cgggggtttt ggtggcggtt ttataatcgt
      301 cttcggcttc gctgccagtt ttacgaccca ggcatcggta tcgggcgtgc cacccttctt
      361 tgtgctgttg aaggtcccgg caaagacgta ctcggacgct gtcgtctgga tgaccgacgc
      421 cgcccggtcg cccgggttcc tgcccccgat cgtcaggtcg ccctcgacgg ccccctcgcc
      481 gtcgagcttc acgatccagg cgtcgttatc gagcggagac tctccggtcc tgccggcaaa
      541 gaggtatccg ccgtcgcgag tggcgatgat cgattcggcc gactcgttct catccgcgtt
      601 cccgtaggtc cagttccagg cgaccttccc gtccggtctc atcctgacga ccagggcatc
      661 gtcgtctgtc cttccggaag cagtcctgct catgtaactc cctgcaaaga caaaacctcc
      721 gtccggcgca aggacgatcg accgggcagt atcgttctcc gcgccgccga aggtcacgtt
      781 ccaggtctcg ttcccggacg agtcgatctt ctggacgaac gcatcggccg caaacgctcc
      841 aaacgactcc gtactccccg caataccgat atcaccggac ggtgccgcga cgaccgcgta
      901 tgccgtatcg ttcagccccg gttctccgaa ggtacggctc cactcctccg acccggaacg
      961 gttgagcttg atcaccagcg catcggtgtc cggctctccc cagaatgccg tttttccggc
     1021 aacgatgtaa cccctgtccg ccgtctcggc gaccgcgtat gccgtggcgt tgtcctcagg
     1081 cccgccgaac gtctcgttcc atatgatatc gcccgtcggg ctgaacttca cgaccagaac
     1141 atcggtatct ttccggtctt cccccattat gtaggtgaac gcccccacga agaggtagtt
     1201 cccgtccgat gtgttgatca ccgatcgggc cgtatccata ccgcgatcac cgtaagtcct
     1261 gttccatcgt tcgtcgcccg aacgggagag atcgaccacc caggcgtctg cttctccggc
     1321 gccgaacgat tcggtctcgc cggaaaagac gtatccccta tctcccggaa tctggatgat
     1381 cgagtatgct gaatcgttcg caccaggttt gccgtaggtc ttattccata caacggagag
     1441 gtccaccgca gcggcaccgg cgacggtcgc cagtgcgcag gttaacagta tgaaagccag
     1501 aatgctctct cttctcatct cctcaactcc tcaagcagtc ctttcccgca ggaaggtgct
     1561 ccggagcgat gagatccttg aggcgtttta cgttccgccg ggtgaaggga agagatgacc
     1621 gtggtagatc gtgtggcact tttcgggatg atatgctgat gcccgaacaa gatttaccgc
     1681 cagccggtct tctgcgaatc agcgatggag tagtcgatgc accctcgatc tgcgtcgaga
     1741 gcacaatatc tgtaataata tattaatttc tccatatact cggatgaacc tgaggcgatc
     1801 tccaccttgc cggatatcct gccgtcactt ccccttccgg gtagtcctgt gccagtggag
     1861 cggggtcagg cccggtacag cggtgggaaa cgtcccttcg gccgcgcgtc ggacgtcttc
     1921 gatcgagtag aatgccttgg gattgaactc atggatggcc ttctccacat ccttgatgtt
     1981 cttcctcttg atcaccgaga agatgacttt gccggggccc tgttttccct gggcgtcgag
     2041 caccgtcacc ccgtagccgg caccgcggag gtaatcgatg aggttcgttg cgtcccgctg
     2101 ggtgatgacc cggatgaggg caagccccat cgccagcctc tcctcgacga gcatcccgac
     2161 gtagtttccg gtcgcaaacc cggccgcata ggcaaagtag ttgagcgggt tcgtgaggtt
     2221 ctgaaagatc tggccgacgg caatgatcca gatgaagacc tcgaagaacc cgaggaccgg
     2281 ggcgaccatc ttcatccccc gcgagacgaa gatgatccgc atcgtcccga tagtgacgtc
     2341 gcagatccgc gcaaagaaga taaacgccgg gactatgacc agggataaga cctccgggtc
     2401 gatacccggg acggtaccga gcatggatca ttgttgatga acgagaaata taattctatc
     2461 catatgtaaa tacataccgt cgagaaaaag ctcatgaagt cgcgctttcg aggatattga
     2521 cggataatta cagactatgc cggtggaatg aagttgagat ccttttgtag gcatagcacc
     2581 cgccgggcat ctttatttac ccggactctc tcgatctctt cgtcgacctc gccggtgctc
     2641 tcctcccccg gaggttcggt tcgacgaaca accgggaggg ttcgtcgaca tgtgctccca
     2701 tcggtgatat ccctgaagat cccacgggca ggctcgttcc cggcggcatc ggtcatccgg
     2761 ttgtaaatct ggacggttcc aattataccg gggtctaccg gcatgcggca ggaggaggga
     2821 ggtcacggcc tctgccggga ttcagggtgc cggccgtcgc gcggggggaa ggggtcgccg
     2881 gcatagcatc gatgaaatcg aacttttttc aaaaaatcag gcaattacgc aatattattg
     2941 gcataccgtg gcgtttaaat tggggataat atccatacac ctaaatatca ctccatgaaa
     3001 gctacccctg aacgcatgtt tcaggtgatc gaagtgagta gcagacggat caaggaaatt
     3061 cgctcgatgt catctttttt cgcgctgatt gtgcttatct gcgcgatgct gatagcaccg
     3121 gcagctgcag actccctgcc ggacagggtc tatgtgaacg catcgtataa cgatgaaacg
     3181 cccgactacg ggaaactgaa cttctcgagc atccaggagg ccgtcgataa tgtcacggat
     3241 ggcggcgagg tgtgggtcta caacgggacc tacaacgagc cgatcgtgat cgacagggcc
     3301 atgaccgtca cgaccgtctc ccaccttgaa tgccccggcc tcgacggcgt aatcatcgac
     3361 gccatgggtg cgcaggcagg catcgagatc gacgcagacg aagtctccat ttccggcttt
     3421 gaggtcgtga acgcctccgg ggtcgggatc tacgcctaca acgccggggc ggtcaccatc
     3481 gagtacaact cagtcacggt cgagaacgac acctacgaac tcacctgcgg tatcctcatc
     3541 gagggcggcg agggagtctg catcggcaac aacgcggtgc gtgtcatcgg gagcagcgct
     3601 caggcgggca tcatggtcaa tggcgtcacc gaagccaacg tccacgacaa ccacgtcgtg
     3661 gcggtctcgc ggcaccaggg tctcgaagcc gtcgagatgg aacccaaccc ggttgttgcc
     3721 gccctcttca cggaggctga tgaaccgttc atcgctgcca gggcgaccgt cacacagcag
     3781 gcgtctgccg gggtcatcat cgaagagagt tcctctgttc tcgttgagcg caacaggatc
     3841 gtcgccgtgg gtaactgcac cgaggacgct ctggatctcc actactcggc cgtcgaggcc
     3901 tggggtatcc ggtccggcgg caactcgaag aacctgaaga tcctggagaa cgacgtcacg
     3961 gtcgtcggaa aggctctcga cacagcctgg acggtcggta tcctcggggt cggagacctc
     4021 gcgctcgtcc agagcaacac ggtcgaggtt atcgccaggt cccgggtcgt cctgccggta
     4081 ggcatcgtga tctccgaggc cgagaagggg caggttctcg ataacgagat cgaggtagag
     4141 gccgcctgca tcggcaaatc cgacggagaa gtcagtccgg aggccaccgg catcatagcc
     4201 gtcgagtccc cgaaagccca ggttctcagg aacgacgttc tgtacacgct catcctcacc
     4261 gacgcaggcc agctggccat gacgtcgaca gagggtattt acattggcgg atgcgacggg
     4321 tcccgggtga gcgagaacat cgtcctcatc cagaccggga tcttcaacct gccggtcgac
     4381 ggtgacgaat ccaccgaaga cgaagagccc ctgaccctgg gggtcgcgag ggtcaaaggc
     4441 atcggcgtcg aagcgagcga caaacccgag atctgcgaca acgtcgtgca cgccttcggc
     4501 gctgtcatga cctacgatga gactgccggg gatctcaatg tgggcgccct ctccgctctc
     4561 gtcatgacag gcatccacgt ctggagctac tccgacgacc tcctcgaaaa ccccgtggtc
     4621 cgcgagaacg tggtcgtggt cagggcgcag gtgcagacca atctggttcc cgaactggaa
     4681 ctcgatgaag aagaggtaaa agccgccctt gcaggcaaca gcgccctgct cgcccgttac
     4741 gtcggcatga tgcaggagag cgaggagttc tccgtcgcaa cgtgcctcgc ggacaccaaa
     4801 ggcctgtcgg ccctcacgga gagcgacctt gagttcgaac cggatatcga tgaggcgacc
     4861 ctcgctgccg cactcacggt aacagccggc atcgtcctcg aagatgtcct gaacccggag
     4921 gtctgctgca actacgtgcc ggtgtaccag gacgtcgccg tcattgtgtt tgaccggccg
     4981 gtcgatgacg ttggcatggt gctccagact cctctctcgt tagggcttat cgaccaggcc
     5041 ttctcggatg agcaagtgca gcaggcgttc ctgctcggcc tcatcgacga ggacgagagc
     5101 gcatggcaga acctctctaa agacgacaga gcggcgatca cacaggccgt ccttgccgga
     5161 gataccggcg tccttgcagc ctacaccgat gacgagacgc tcgccgggta tcaggcactc
     5221 atggaagacg gggaagcgtt cgagttcctg aacacaaccc ggcttgcggc cgcgatcccg
     5281 ctctcggtga gctgcggcgt ctacgtcaac gacgccgagg gagacgtggt gctccacgac
     5341 aacgagatcg tggtagagac caacgccgcc ggcctcgcag ccgcgcaagg cggcgacctc
     5401 gatgcgcccg acgcggcggc gggctccgcc ggcattgtag cggcgttcgg cttcgccgga
     5461 acaggtgatt ccatcagcgt cgaagagaac tgcatccatg tgatcaccaa cggcatgttc
     5521 ctgggccagg ccatgggggg tgaaaacgag accgccgata cagcagcagg caccgcaaac
     5581 ctcttcctcg gagtgggagt tcttgccgat gcagacggcg agcacgtcgt gaacaacgac
     5641 attactgtcg cacagagaag ccgggcccag accctggcgt tcaatatagt taaggatagg
     5701 acggccctca gcgtcgcggc tacggcgggc gtgggcatcg gtatcgtcct cgatccatac
     5761 gatgtcttcg atcccgagac ctacgagccc gctccccctg cgccggacga gtggttcgca
     5821 agcgtcatcg agggtaacac cattgccgtc acgaacgata tcaacacctt ctcgctcgcc
     5881 gagatgctgc tccctgcacc cctcgacggg tcgtcgaacg cgct
//
LOCUS       DAOSEE010000126         5915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_4823_length_5915_cov_278.958532, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000126 DAOSEE010000000
VERSION     DAOSEE010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5915
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4823_length_5915_cov_278.958532"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="PLI76_08970"
     CDS             <1..1098
                     /locus_tag="PLI76_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system ATPase subunit"
                     /protein_id="HOI62205.1"
                     /translation="IPLFLYHRRHQNIKTNIFFDEPALNSLAITLAQRSGKHVSIGSP
                     LVDATLPDGSRLQLTFGSEVTTRGTSFTIRKFRETPFTPVELMETKTFDVDQLVYFWM
                     AIENNKSLLFVGGTASGKTTSLNAVALFIPPLAKIVSIEDTREITLYHENWVATVTRE
                     RISEGLGATIGMFDLLKAAMRQRPEYILVGEVRGNEAQTLFQAMNTGHTTFSTMHAGG
                     VDAAIHRLENAPLNVPRNMLQALDIISVQALTHRGYDRARRCREIVEIAGIDPITGSL
                     QVNTVYEYNPVPDTFSYSGRSRILGEIMEYRGWSRTRLDEEMQIRRSVLLAMQNQGIK
                     DYRSVARIIQAFTIEPDRVLASLDDLGRLIR"
     gene            1143..3035
                     /locus_tag="PLI76_08975"
     CDS             1143..3035
                     /locus_tag="PLI76_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOI62206.1"
                     /translation="MERDPIRYRSLREDLVAANLGVTLDRYLLLTFLASGLFGAFWAI
                     LVFLVLRFAVLPRMSIGIYNVFAIHLPEVMLVDPMISILQVVASLVVFLITAYAASLV
                     FLQYPTLVKANRATRINLLLHHAVAYMYAMRQGGAEMMGVFRAIGGNSAVYGEAAHEF
                     RRVIRDTDYFGYDQIAALRHLQETTPSEKLAEFVQDLVSVVESGGDMLAFLDARVRIY
                     QEEARFEQKTFLSTLQLVAEAYVTLFVAGPLFIIIVMVVMGFMGSTPILQLSVIIYVL
                     VPVGSLLFILFLDVISIKTEGIERYTETRWLDEFDDVRVEERTGDEVQVRQLQRYDRI
                     RNIRAFLRHPLKAFLVDPNRTFYVTVPVALVYVLLAFFMTPAYTDVEVLIDVLDDHII
                     IALLIILVPFGAFHWFWQKTVMGLEASIPEFLNRLSGINQVGLTLVQAITIVVKADLG
                     VLTYEIKKIKRDIDWGASIQDALVRFEERIRTPAIARAVTLITTASRMTGDIGEVLNI
                     AARDAAMSETLKRERRGEMFIYVAIVYLVFIVFLFVVIVIDTQFLSALAETEILSPGG
                     LSAGISFGKTPVITFERLLFHACLIQALFSGLIAGEMGEGSLRAGVKHAAVMVFIAFV
                     VFTIAL"
     gene            3176..3328
                     /locus_tag="PLI76_08980"
     CDS             3176..3328
                     /locus_tag="PLI76_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62207.1"
                     /translation="MCTVGGPVDIEFNERVKGRNYRCKECGERFKGIGKRPMCPSCQS
                     EDVEEA"
     gene            3312..3848
                     /locus_tag="PLI76_08985"
     CDS             3312..3848
                     /locus_tag="PLI76_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOI62208.1"
                     /translation="MSKRPEGSASGSPPTAPGEAAVPLAREILIIRRGGSFLLVGKAG
                     SRFTLCIETPDDEYCLAVDPDDLVVVSMPEGGPIEQARMMLELVRQYHIPLVVLPKDH
                     PGSKRLSMVVSVAPEILLACDVRRGTHPEQHLLCSSAELSGLSLAGVPGGITLKQLPP
                     GATVERLNPEKSSTDKQQ"
     gene            3900..4229
                     /locus_tag="PLI76_08990"
     CDS             3900..4229
                     /locus_tag="PLI76_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit subunit G family protein"
                     /protein_id="HOI62209.1"
                     /translation="MKTDVLKSIRETEEEYQAMIRDAQAGRKKSLSDAELEADNLVQK
                     AQSDAGDYRNRRLAEARAQAQNRYAEIVREGEARAETLKAQGGKNLTRAVDFVVSRFK
                     EHLHVKA"
     gene            4216..>5915
                     /locus_tag="PLI76_08995"
     CDS             4216..>5915
                     /locus_tag="PLI76_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HOI62210.1"
                     /translation="MLRPEGMRRLLIAVPKGEIDTVIRELYRHNIYHIEDFVPESESP
                     DELSIGHPLPGASDAASALIKVRAIENACGIDPDSVEIKEKIPASKVRERIRTDLPAI
                     QKEVEGLVATRSKLEAQKKEHEQRAKELEPFAALPLDLELYRGYIRFTVFTGHIRHDV
                     VIDVPHEKYFSDKVDGNMIAVVVPNEHREEVERTLLDSGFQAIPVPEEAGAPADRLKN
                     HIDEAREATTGIAGVNQRLAEIRDRHADLLVACDELLTADVERAEAPLRFATTEETFI
                     TEGWVPEDRADGIRAALEKATNGRIYIGELAIDDDAKVPVEYENPSFASPTQVLMDIY
                     ARPRYSELDPTLMVAIVYPIFFGLILGDVGYGAILLALSFGLRKFLKGDEGRMLLSVL
                     TYSSISSIIFGVLYSEFFGAALPWPALIFNRHLNIGGAAGGHPPQITELMILAIWIGI
                     LHITLGRILGMVNARKLHHGRHAAKAAIANAGWIGTMWGILIMIWSLYAIPLMPDLTG
                     LPVVAMGFNVAAVVGAVLLVAGILGIAQENALELVEIPTIISHVLSYARLVAVGLSSV
                     A"
BASE COUNT         1174 a         1839 c         1725 g         1177 t
ORIGIN      
        1 ataccgctct tcctctacca tcgccggcac cagaacatca agacgaacat cttctttgac
       61 gaaccggcat taaactcgct cgcgatcacc cttgcgcaac gttcaggcaa gcacgtctcg
      121 atagggagcc cgcttgtgga tgcaaccctc cccgacggtt cgcggttgca gctcaccttc
      181 ggcagcgagg tcaccacccg gggtacatcg ttcacgatcc ggaagttccg cgagacgccc
      241 ttcacgcccg tcgaactgat ggagacaaag accttcgacg tcgaccagct cgtctacttc
      301 tggatggcga tcgagaacaa caaaagtctg ctcttcgtcg gcggcaccgc gtcgggaaag
      361 accacatcgc tcaacgcggt cgccctcttc ataccgccgc ttgcaaagat cgtctccatc
      421 gaggataccc gcgagatcac gctctaccac gagaactggg ttgcgaccgt cacgcgcgag
      481 aggatatcgg aagggctcgg cgccaccatc gggatgtttg acctcctcaa ggccgctatg
      541 cggcagcgcc ccgagtacat actggtcggc gaagtcaggg gtaacgaggc ccagactctc
      601 tttcaggcaa tgaacaccgg gcatacgacg ttctccacca tgcacgccgg gggcgtcgac
      661 gcggcgatcc accgcctgga gaacgcaccg ctcaacgtcc cacggaacat gctccaggcg
      721 ctcgacatca tatccgtcca ggcgctcacc caccggggat acgaccgcgc gcggaggtgc
      781 cgggagatcg tcgagatcgc cgggatcgat cccatcaccg gcagcctcca ggtgaacacg
      841 gtctacgagt acaaccccgt gcccgatacc ttctcctact cggggcgatc acggatcctc
      901 ggcgagatca tggagtaccg cggctggagc cggacccgcc tcgacgagga gatgcagatc
      961 cggcgttccg tccttcttgc gatgcagaat cagggcatca aagactacag gtcggtcgcc
     1021 cggatcatcc aggcctttac gatcgagcct gaccgggtgc tcgcttctct tgacgacctc
     1081 ggcaggttga tccggtgatc ctgcctccct gcatacggat ccgccgtctt atccgccggt
     1141 tggtggagcg cgaccccata cgctaccgga gcctccgcga agaccttgtt gcggcgaacc
     1201 ttggtgtcac cctcgaccgg tacctcctcc tgacgttcct cgcctccggg ctcttcggcg
     1261 ccttctgggc tatcctggtc ttcctcgtgc tgcgatttgc agttctcccg cggatgagca
     1321 tcgggatcta caacgtcttt gccatccacc tcccggaggt catgctggtg gatcccatga
     1381 tcagcattct ccaggtcgtc gcgagcctgg ttgtcttcct catcaccgcc tatgcggcct
     1441 ctctcgtctt cctgcagtat ccaaccctcg taaaggcgaa ccgggcaacc cggatcaacc
     1501 tgctgctcca ccacgccgtc gcctacatgt atgccatgcg ccagggcggg gcggagatga
     1561 tgggggtctt ccgggcgatc ggcgggaact ctgcagtcta cggcgaagcg gcccacgaat
     1621 tccggcgcgt tatccgggat accgattact tcggctacga ccagatcgcg gctctccgac
     1681 acctgcagga gacgaccccg tcggagaaac tcgccgagtt cgtccaggat ctcgtctccg
     1741 tggtcgagag cgggggggat atgctcgcct tcctcgatgc ccgggtccgc atctaccagg
     1801 aagaggcccg gttcgagcag aagaccttcc tctccacgct ccagctcgta gccgaggcat
     1861 acgtgacgct cttcgttgcc ggcccgctct ttatcatcat cgtcatggtg gtcatggggt
     1921 ttatgggcag cacgcccatc ctgcagctct cggttatcat ctatgtcctg gtcccggtcg
     1981 ggtcgctcct cttcatcctc tttcttgacg tgatatcgat caagaccgag ggcatcgagc
     2041 ggtacaccga gacgcgctgg ctcgacgagt tcgacgacgt ccgggtcgag gagcggaccg
     2101 gcgacgaagt acaggtccgg caactgcagc gctacgaccg catcagaaac atccgcgcct
     2161 tcttgcgcca tccgctcaag gcattcctcg tcgatccgaa ccgaacgttc tacgtgacgg
     2221 tgccggtcgc gctcgtctac gttctccttg ccttcttcat gaccccggcc tacaccgacg
     2281 tcgaggtgct gatcgacgtc ctcgacgacc acataatcat tgcgctgctt atcatccttg
     2341 tcccgttcgg cgccttccac tggttctggc agaagacggt gatgggcctg gaggcctcca
     2401 tccccgaatt cctcaaccgt ctctcgggta taaaccaggt gggcctgacc cttgtacagg
     2461 ccatcacgat cgtggtgaag gcggatctcg gggtgctgac ctacgagatc aagaagatca
     2521 agcgcgatat cgattggggt gcgagcatcc aggacgcgct tgtccggttc gaggagcgga
     2581 tacgcacccc tgccattgcc cgtgcggtca ccctgatcac gacggcaagc cggatgaccg
     2641 gcgatatagg cgaggtgctg aacatcgccg cccgcgacgc cgcgatgtcg gagactctca
     2701 agcgcgagag gcggggggag atgttcatct acgtcgccat cgtctacctg gtcttcatcg
     2761 tcttcctctt cgtcgtgatc gtcatcgata cccagttcct ctccgcgctc gcagagaccg
     2821 aaatcctctc tcccggcggc ttatccgccg ggatatcgtt cggaaagacc cccgtcatca
     2881 cgttcgagcg cctcctcttc catgcgtgtc tcatccaggc gctcttctcg ggcctgatcg
     2941 ccggggagat gggagaggga tcgctccggg cgggggtcaa acacgccgcc gtgatggtgt
     3001 tcatcgcgtt cgttgtgttt actattgccc tgtaatagtc cccgatctcc acctccggcc
     3061 ggcccgtgcg ggaactcctc tctatcgggc atcagccttc cgcctcaccg tgccgtgggt
     3121 ttcgcgatag aattttatat tctccgctag agatcactct cctggtgata acaagatgtg
     3181 tactgtaggc ggacctgtcg atatcgagtt caacgaacgg gtgaaaggca ggaattaccg
     3241 ctgcaaagag tgcggcgagc ggtttaaggg catcggaaag cggcccatgt gcccgagctg
     3301 ccagtcggaa gatgtcgaag aggcctgaag gcagcgcatc cgggagccct cccaccgcgc
     3361 cgggcgaggc tgccgtcccc ctcgcgaggg agatcctgat catcaggaga ggtggctctt
     3421 ttctgctcgt ggggaaggcc ggttcccggt ttaccctctg tatcgagacc cctgacgacg
     3481 agtactgcct tgcggtcgac cccgacgatc tcgtcgtcgt ctcgatgccg gagggcgggc
     3541 ctatcgagca ggcacgcatg atgctcgagc tggtccggca gtaccatatc ccgctcgtcg
     3601 ttcttccaaa agatcatccg gggtcaaaac ggctttcgat ggtcgtctcg gttgctccgg
     3661 agatcctgct tgcctgcgac gtccggcgag gaacccaccc cgagcagcac ctcctctgct
     3721 cgtcggcaga actctccggg ctctcgcttg ccggggttcc cgggggtatt acactgaaac
     3781 aactcccccc cggggcgaca gtagaacgtc tgaatccgga gaaatcctct acggacaaac
     3841 aacaataaat atatgctcat tttcatcaaa tacttataag ccgtgagaag gagggtttga
     3901 tgaagaccga tgtcctgaaa agcatcaggg aaactgaaga agagtatcag gcaatgatcc
     3961 gtgatgcgca ggccgggagg aaaaaaagcc tctcggatgc cgagttggag gctgataacc
     4021 tggtccagaa ggcacagagc gatgccgggg actacaggaa tcggcgtctt gcagaggcac
     4081 gggcccaggc gcagaacagg tacgcagaga tagtcaggga gggtgaagcg cgcgcagaga
     4141 cgctcaaagc gcagggaggc aaaaacctca cacgagctgt agactttgtc gtctcgcggt
     4201 tcaaggagca cctgcatgtt aaggcctgaa gggatgcgcc gactgctcat cgcagtcccg
     4261 aagggtgaga tcgataccgt tatcagggaa ctctaccggc acaacatcta ccacatcgaa
     4321 gattttgtgc cggagagcga gtctcccgat gagttatcga tcggtcaccc gctcccggga
     4381 gcaagcgatg cggcctcggc gctcatcaag gtcagagcca tcgagaatgc gtgcgggata
     4441 gatcccgata gcgttgagat caaagagaag attcccgcat cgaaggtcag ggagcggata
     4501 aggacggacc ttcccgccat ccagaaggag gtggaaggcc tcgtcgcaac acgctcgaag
     4561 cttgaggcgc agaagaaaga gcacgagcag cgcgcaaaag aactcgaacc gtttgccgcg
     4621 ctccctcttg acctggaact ttatcgggga tatatccggt ttactgtctt taccgggcat
     4681 atcaggcacg atgtagtcat cgacgtcccc cacgagaaat atttttccga caaggtggac
     4741 ggcaacatga tcgccgtcgt ggtgccaaac gagcaccgcg aagaggtcga gcggactctt
     4801 cttgattccg ggttccaggc gattcccgtc cctgaagagg cgggagctcc ggctgatcgc
     4861 ctgaagaacc atatcgacga agcccgggag gccactaccg ggatcgccgg ggtcaaccag
     4921 aggcttgcag agatccggga caggcacgct gatctcctgg tagcatgcga tgaactactc
     4981 acggccgacg tagagcgggc ggaagccccg ttgcggtttg ctaccacgga ggagaccttc
     5041 atcaccgaag gctgggtgcc tgaggaccgg gcggacggga tccgggcggc actggagaag
     5101 gccaccaacg gtcggatcta catcggggaa ctggcgatcg atgacgacgc aaaagtcccg
     5161 gtggagtacg agaacccgtc gtttgcctca ccgacccagg tgctcatgga tatctacgcg
     5221 cgaccccggt actccgagct cgacccgaca ctgatggtag ccatcgtata ccccatcttc
     5281 ttcgggctga tcctcggcga cgtggggtac ggcgcgatcc tgctcgcctt aagcttcggg
     5341 ctccggaagt tcctgaaggg tgacgaagga aggatgcttc tatcggtgct cacgtactca
     5401 agtatatcga gcatcatatt cggggttctc tacagcgagt tctttggggc tgcactcccg
     5461 tggccggcgc tgatcttcaa tcggcacctt aacatcggag gagccgcagg cggtcacccc
     5521 cctcagatca ccgaactcat gatcctcgcg atctggatcg gcatcctgca tatcacgctc
     5581 gggcgcatcc tcggtatggt caacgcccgg aagctccatc acggaagaca cgcggcaaaa
     5641 gcggcaatcg ccaacgccgg gtggatcggg acgatgtggg gtattctcat catgatctgg
     5701 tcactctacg ccatcccgct gatgcccgac ctgacgggtc ttcccgttgt cgccatgggg
     5761 ttcaacgttg ccgccgtggt gggagccgtc cttctggtgg caggcattct cgggatcgcg
     5821 caggagaacg cactcgagct tgtcgagatc ccgacgatca tcagtcatgt attgtcgtac
     5881 gcccgtctcg tggcggtggg cttatcctcg gtcgc
//
LOCUS       DAOSEE010000127         5911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_4829_length_5911_cov_223.459016, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000127 DAOSEE010000000
VERSION     DAOSEE010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4829_length_5911_cov_223.459016"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="PLI76_09000"
     CDS             complement(<1..626)
                     /locus_tag="PLI76_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(10)-N(2))-dimethyltransferase"
                     /protein_id="HOI62211.1"
                     /translation="MDLVEVTEGRTRFSVPEQDPHLQFPPGSGPVFYNARMEMNRDAT
                     VLLLSVLRPENYLDAMGASGARGLRVACEVGIPVTINDRSQRAVDLARQNIEALGLVA
                     DVTRRDANALMSERKFDAVDLDPFGTPAPFVDSAARSAERYLFVTATDTAPLCGAHLK
                     AGMRRYFSRPRNTEYHAEVGLRTLLGFVVREVIKYDRGVEPLFCYAHE"
     gene            696..770
                     /locus_tag="PLI76_09005"
     tRNA            696..770
                     /locus_tag="PLI76_09005"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:731..733,aa:Arg,seq:cct)
     gene            834..1673
                     /locus_tag="PLI76_09010"
     CDS             834..1673
                     /locus_tag="PLI76_09010"
                     /EC_number="2.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HOI62212.1"
                     /translation="MSVSAFVRITRPHNAVVAGFTALIGYLVATGTLILPSLLLAAVV
                     ALITAAGNVINDVYDIDIDRINRPDRPIPSGAISLSGAKIYAVALFAGGIALATLTTA
                     LCLLIALANALILILYAAWLKRTPGIGNVAVAYLTASVFLFGGAFAGIEGLIQNISLA
                     AIAFLATLAREILKDAEDVDGDAAGGARTLPMIVGIRRTGLFAFACACGAVLASLLPS
                     GDWWGPFYLAAIAVVDIIILAGAARGVRCTTPDCVRESRATSILRAGMFAALAVFAVA
                     AVT"
     gene            1760..2170
                     /locus_tag="PLI76_09015"
     CDS             1760..2170
                     /locus_tag="PLI76_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOI62213.1"
                     /translation="MKVYRHGNTYIAPRGSFFDGNVKIDGNFIAPPETHIWGNMVVAG
                     CLELGPYSTVGGYVEAESIVIGHDAKIKGPLRIQKDATVCDNACLHSIRAGGNIIIRP
                     GVRVGDVNSAETIYVYGKVTSERLFGRAVKVYGT"
     gene            complement(2135..2839)
                     /locus_tag="PLI76_09020"
     CDS             complement(2135..2839)
                     /locus_tag="PLI76_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HOI62214.1"
                     /translation="MSILDSGAWVGLMTLIGEITFFLILGMVLAAVALAIIATASITR
                     GKFYLPRILIPGMVLLEGLVKAICKLLGLDDKDLITFFITLRNTMNTKAFAGTPVEQR
                     AVFLPQCLRSAECPAHLTPEGLKCRHCGRCTVGENVAWLEGIGYRVFIVPGSTFIKRM
                     VKKYRPKAIIGVGCLMEVKDGIDMSDRIGITAIGVVNFKDGCVETVADWAGVRDAALL
                     GIEPPSGAVDFHGPAE"
     gene            complement(2836..3897)
                     /gene="mtnA"
                     /locus_tag="PLI76_09025"
     CDS             complement(2836..3897)
                     /gene="mtnA"
                     /locus_tag="PLI76_09025"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOI62215.1"
                     /translation="MLELDDLCTIAWEEETGSIIYIDQTLLPGRCEQMRCTTVEDLAR
                     AIRRLEIRGAPALGIAGAMGVALAAARSTERSLDRFLADVGRAADLLRGTRPTAVNLA
                     WGIDRVIRRIAVAASVDEAKTVAAAEAKAVADEDELVCRRLGAFGEGLLPDDCTVLTH
                     CNAGALACRCWGTALGVIRSAVAAGKNVRVIACETRPLNQGSRLTCWELLRDGIDVTL
                     IPDSSAAYLMRKGRVDLVIVGADRITRDAVFNKVGTYMHAVTARHHGIPFYVAAPLST
                     FDLSRVESEIVVEERDRSELACSGDRQLAPDGVNVLNYAFDATPLDLVDAIVTEIGVL
                     RPPYAESFRLAGRREGVRP"
     gene            complement(3900..5174)
                     /locus_tag="PLI76_09030"
     CDS             complement(3900..5174)
                     /locus_tag="PLI76_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOI62216.1"
                     /translation="MADVVVIGAGVAGIQAALDLADHNIHVHLVEREPTIGGHMAQLD
                     KTFPTNDCSMCILSPKMVDVARHPNVTIHTCSEVEGIEGEVGTFRVRVRKHPRYIIES
                     ECNGCGDCIQICPVEVYNRFDAGIGVRKAIYKPHAQAVPDIVIKDKDHCIECGLCYDV
                     CGREAILHEDEERVVDIEAASIVVATGYATFDAGKKAQLRYLLIPDVITSLEFERMIN
                     ASGPTGGRLKRLSNGNVPRSIAFVQCVGSRDISAGRPYCSGVCCMYAMKNAMLIREKN
                     PEIEVTVFYNDIRAYGKGYEEYYERAKDLGVRFIRGFPGEVLEGNDCLMMTAENTETG
                     EVETVHPDLVVLSVGLEPARGAEEVARMLGIPQDESGFFEVADQKLGPVLTVRPGIYV
                     AGTATAPRDIPDSVAMGEAAAMRAYLDTVRAG"
     gene            complement(5167..5658)
                     /locus_tag="PLI76_09035"
     CDS             complement(5167..5658)
                     /locus_tag="PLI76_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOI62217.1"
                     /translation="MGSGTVVGNRVFIAENATVIGDVTLADDVNIWFGAVVRADRDTI
                     TVGSGSNIQDNAVVHTTIGFPVDIGSDVSVGHGAILHGCIIHDRVLVGMGAIVLNGAV
                     VGEGSIIGAGSVVTEGKEIPANSLVLGVPGKVVRETTPEQRDGITRNAREYVKLAGRY
                     RRG"
     gene            complement(5726..>5911)
                     /locus_tag="PLI76_09040"
     CDS             complement(5726..>5911)
                     /locus_tag="PLI76_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62218.1"
                     /translation="GAGDAGEVSGYPLPQARAGWGAGEKRLEVPGREAPASGIRIASS
                     RCDSSPLRSTRGRTTSR"
BASE COUNT         1016 a         1961 c         1843 g         1091 t
ORIGIN      
        1 tgctcgtgcg cgtagcagaa gagcggttcc accccgcggt cgtacttgat cacctcgcgc
       61 acgacaaacc cgagaagtgt ccggaggccg acttcggcgt ggtactcggt gttccgcggc
      121 cgggagaagt atcggcgcat gccggctttc aggtgcgccc cgcagagcgg cgcggtgtcg
      181 gtggcggtga cgaagaggta tctttcggcg ctccgggctg cagagtcgac gaacggcgcc
      241 ggggtcccga aggggtcgag gtcgacggca tcgaactttc gttcgctcat cagcgcgttt
      301 gcgtcgcgcc gggtgacatc ggcgacaagg cccagggcct cgatgttctg ccgggcaagg
      361 tcgaccgccc gctggctcct gtcgttgatc gtcaccggaa taccaacctc acaggccacc
      421 cggagccccc gggccccgga cgcccccatg gcgtcgaggt aattctccgg cctcaggacg
      481 gagagcagga ggacggtggc atcccggttc atctccatac gcgcgttata aaagaccgga
      541 ccgctccccg gcgggaactg gaggtgagga tcctgctccg gcacagagaa ccgggttctt
      601 ccctccgtaa cctcaacgag atccatactt ctggaaaagg tggatgctcg atatccttat
      661 accttcttgt gagaaacact ggtactccgg tgtatgggct tgtggcctag ccaggatatg
      721 gcgtcagcct cctaagctga atgtcggggg tccgaatccc cccaagcccg tcgtttctcc
      781 gaaaaagacc atttggttat ttatcttccg cccgagaacc tgatccgttg agcatgagcg
      841 tctctgcctt cgtcaggatt acccgcccgc acaacgccgt cgttgccggt tttaccgccc
      901 tcatcggcta cctcgtcgcc acgggaaccc tcatcctgcc ctccctcctg cttgccgcgg
      961 tcgtcgcgct catcaccgcc gcggggaacg tgatcaacga tgtctacgac atcgatatcg
     1021 atcgcatcaa ccgtcccgac cggccgatac cctccggggc gatcagcctt tccggcgcaa
     1081 agatctacgc ggtagcgctc tttgccgggg gcatcgccct cgccaccctg acgacggcgc
     1141 tctgccttct gatcgccctg gcaaacgccc tgatcctgat cctgtacgcc gcctggctga
     1201 aacggacacc gggcatcggc aacgtggccg tcgcctacct gacggcaagc gtcttcctct
     1261 tcggcggtgc ctttgcgggc atcgagggac tcattcagaa tatatctctg gcggcgatcg
     1321 cgtttcttgc aacccttgcc cgggagatcc tcaaagatgc ggaagacgtg gatggggatg
     1381 ccgcgggggg cgcacgcacc ctccccatga tcgtcgggat ccggcggacg gggctcttcg
     1441 cgttcgcctg cgcctgcggc gccgtgctgg caagcctcct cccctccggc gactggtggg
     1501 gccccttcta ccttgccgcc atcgcggtcg tcgatatcat catactcgcc ggggctgccc
     1561 ggggggtcag gtgcacgacg ccggactgtg tcagggagtc cagggcgaca tcgatcctgc
     1621 gggccggtat gtttgcagcg ctcgcggtct ttgcggttgc tgcggtgaca taggctggcg
     1681 agatttatcc atatcgcccc acgatgcacc ggaaaatccc actgatcatt ttatgtattc
     1741 cggggcagag ttagtaccga tgaaagttta ccggcatggg aacacctaca tagcgccgag
     1801 aggttccttt tttgacggaa acgtaaagat agacgggaac ttcatcgccc ccccggagac
     1861 gcatatctgg ggcaacatgg tcgttgccgg ttgcctcgaa ctcggaccct actccacggt
     1921 aggcggctac gtcgaggcgg agagtatcgt catcggtcat gacgcgaaga tcaaagggcc
     1981 gctccggatc cagaaagacg ccaccgtatg cgataacgcc tgcctccact ccatccgggc
     2041 gggcgggaac atcatcatac ggcccggcgt cagggtcggc gacgtcaaca gcgccgagac
     2101 gatatacgtc tacggcaagg tcacgagcga gcggttattc ggcagggccg tgaaagtcta
     2161 cggcacctga tgggggttcg ataccgagca gggcggcatc ccgcacaccg gcccagtcgg
     2221 caaccgtctc cacgcagcca tccttgaagt tcacgacgcc gatggcggtg atccctatcc
     2281 ggtcggacat atcgatccca tccttcacct ccatgaggca gccgacgccg atgatcgcct
     2341 tcgggcgata cttcttgacc atccgtttga tgaacgtcga acccgggacg atgaagacgc
     2401 gatacccgat cccttccagc cacgctacat tctctccgac cgtgcagcgg ccgcagtgcc
     2461 tgcatttgag gccttccggc gtcaggtgag cgggacactc cgccgaccgc aggcactggg
     2521 gcagaaagac cgcccgctgt tccaccggcg tcccggcgaa ggcctttgtg ttcatggtgt
     2581 ttcgaagggt tatgaagaac gtgatgaggt ccttgtcatc caggccgaga agtttgcaga
     2641 tcgccttcac gagcccctcg agaaggacca tgccgggtat aaggatccgg gggaggtaga
     2701 acttcccccg ggtgatcgag gcggtggcga tgatagcgag agcgaccgcc gcaagcacca
     2761 tgccgaggat gaggaagaac gtaatctccc cgatgagggt catcagaccg acccaggccc
     2821 cggagtcgag aatgctcatg gccggactcc ctccctcctc ccggcaagcc ggaacgattc
     2881 ggcatacggc ggccggagca ccccgatctc ggtgacgatc gcgtcgacga ggtcgagtgg
     2941 ggtggcatcg aaggcgtagt tgagcacgtt caccccgtcg ggcgcaagtt gccggtcccc
     3001 cgagcacgcg agttcggagc ggtcccgctc ctcgacgacg atctcgctct ccacccgcga
     3061 gaggtcgaac gtggagaggg gcgccgcaac gtagaacggt atcccgtggt ggcgggcggt
     3121 gacggcatgc atgtaggttc caaccttgtt gaagaccgcg tcccgcgtga tcctgtccgc
     3181 accgacgatg acgaggtcga ccctgccctt ccgcatcagg tatgcggccg acgagtcggg
     3241 gatgagggtc acgtcgatgc catcgcggag gagctcccag caggtgagcc gggagccctg
     3301 gttcagcggc cgggtctcgc aggcgatcac ccgcacgttc ttcccggccg cgaccgccga
     3361 ccggatcaca ccgagggccg tcccccagca ccggcaggcg agagcgcccg cgttgcagtg
     3421 ggtgaggacc gtgcagtcgt cgggtaagag gccctcgccg aacgcaccga gcctccggca
     3481 gaccagttcg tcctcatcgg caacggcctt tgcctcggcg gcggcaacgg tcttcgcctc
     3541 gtcgaccgat gcagccaccg cgatcctcct tatcacgcgg tcgatccccc aggcgaggtt
     3601 gaccgcggtc gggcgtgtgc ccctgaggag gtcggcggcc cggccgacat ccgcgaggaa
     3661 ccggtccagg ctcctctcgg tgctccgggc ggccgcgagg gccaccccca tcgcaccggc
     3721 gatgccgagc gccggggctc cccggatctc gagtcgccgg atcgccctgg cgaggtcctc
     3781 aacggtggtg caccgcatct gctcgcacct gccggggagg agggtctggt cgatgtatat
     3841 gatgcttccg gtctcctcct cccacgcgat ggtgcagagg tcgtccaact ccagcatatt
     3901 catcccgccc tgaccgtgtc gaggtaggcc cgcatcgctg ccgcctcgcc catggcgacg
     3961 gagtcgggta tatcccgcgg cgcggtggcc gtccccgcca cgtagatccc gggtctcacc
     4021 gtgagcacgg ggccgagttt ctggtcggca acctcgaaga acccggactc gtcctgcggt
     4081 atgccgagca tccgggcgac ctcttccgct ccccgggcag gctcaagccc gacggagagc
     4141 accacgaggt cggggtgaac cgtctccact tcaccggtct cggtgttctc cgcggtcatc
     4201 atcaggcagt cgttcccctc gagcacctcg cccgggaacc cgcggatgaa ccggaccccg
     4261 aggtccttcg cccgctcgta gtactcctcg tagcccttgc cgtatgcccg gatgtcgttg
     4321 tagaagacgg tcacctcgat ctccgggttc ttctcgcgga tgagcatggc attcttcatc
     4381 gcgtacatgc agcagactcc cgagcagtag gggcggccgg cggagatgtc gcgggagccg
     4441 acacactgga cgaacgctat gctccggggg acgttgccgt tcgagagcct ttttaagcgg
     4501 ccgcccgtcg ggccgctcgc gttgatcatc cgttcgaact caaggctcgt gatgacgtcg
     4561 gggatgagga ggtagcggag ctgggccttc ttcccggcat caaacgtcgc ataaccggtc
     4621 gcaacgacga tgctcgccgc ctcgatatcg acgacccgct cctcgtcctc gtggaggatc
     4681 gcttccctcc cgcagacatc gtagcagagg ccgcactcga tgcagtgatc cttatctttg
     4741 atgacgatgt cggggacagc ctgggcgtgg ggtttgtaga tcgccttccg gacgccgatg
     4801 ccggcatcga accggttgta gacctcgacc gggcagatct ggatgcagtc cccgcagccg
     4861 ttgcactcgc tctctatgat gtagcgggga tgcttcctga cccggacacg gaaggttccg
     4921 acctcaccct cgatcccctc gacctcggag caggtgtgga tcgtgacgtt cgggtgccgg
     4981 gccacatcca ccatctttgg agagaggatg cacatcgagc agtcgttcgt ggggaacgtc
     5041 ttgtcgagct gggccatatg cccgccgatg gtcggttcgc gctcgacgag gtggacgtgg
     5101 atattgtggt ccgcaaggtc gagcgccgcc tggatgccgg cgaccccggc accgatgacg
     5161 acgacatcag ccacgacggt acctcccagc gagtttgacg tattcgcgtg cgttacgggt
     5221 aataccgtcc cgctgctcgg gcgtcgtctc tctgaccacc ttgccgggga ccccgaggac
     5281 cagcgaattc gccgggatct cttttccctc ggtcaccacg ctgccggcgc cgatgatgga
     5341 gccttccccc accaccgcgc cgttcaggac gatggcgccc atcccgacga gcacccggtc
     5401 atggatgatg cagccgtgca ggatggcgcc atggccgacc gagacgtccg acccgatatc
     5461 gaccggaaac cctatcgtgg tgtggacgac ggcgttgtcc tggatgttcg agcccgatcc
     5521 caccgtgatc gtatcccggt cggcccggac aacggccccg aaccagatgt tcacgtcgtc
     5581 ggcgagcgtc acatccccga tgacggttgc gttctctgcg ataaagacgc ggttccccac
     5641 aactgttccg ctccccatag tatgtctact gttggttgcg gcgatacaaa atcttctccc
     5701 ttgcgccggc aaggggagcg gggaatcaac gcgaggtggt gcggcctcgc gtggagcgca
     5761 gcggactgct atcgcaccgg gacgaggcga tgcggatacc actcgccggc gcctccctgc
     5821 cgggaacctc caggcgcttc tctccggctc cccaccccgc ccgcgcctgc ggcaggggat
     5881 atccggatac ctcgccggca tcccccgccc c
//
LOCUS       DAOSEE010000128         5875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_4855_length_5875_cov_239.906186, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000128 DAOSEE010000000
VERSION     DAOSEE010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5875
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4855_length_5875_cov_239.906186"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..492
                     /locus_tag="PLI76_09045"
     CDS             259..492
                     /locus_tag="PLI76_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62219.1"
                     /translation="MVNISRLKYRVEVLGDHDDIRRYGPFNEQKAREFFEVEESLGGT
                     ARIARLEEGIRETWRIVAECGEWGSREAELPAR"
     gene            653..2668
                     /locus_tag="PLI76_09050"
     CDS             653..2668
                     /locus_tag="PLI76_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HOI62220.1"
                     /translation="MDPLLQASSLLLWAGLLKGLQLALWPRLRPVLGDYAYPAAYPAS
                     LLLFALATWYCGLLRIPVALALLLFVALGIYGLYRGDYRGLRGFFAWDAVFLVGFLFA
                     LSVRFANPVIGSFSEQYMNHAFLASIIREPIVPPLDPWFAGGHLTVYYYLGHWLMGCL
                     AVVTGTPSRIAFNLIPATVYGAAFVTLYALGHLFLRRLRWVPLAVLLLVPPSVLWFLA
                     AGGDIYSAFQETNWIISGARFEFPAFSLFLGNAHAFEMAVFNQIFLIFLLGFAWLRWG
                     GLDTRGRGGLALLIALSLGSMPLLSSWDALVYGPAAAVFLLALLLRERDRSTVVAAVA
                     VPAIALLIYIPYYLQLEPAGVGGIGMGFPPTDPLAFIAIWGGFLAVIYIAVARDIRQS
                     PLYLGVALPFLVAGYAALGIVAVPLAYLIRRRQRSFADILSLAGLLVLALCDVFYIKE
                     MLGGDYSRFNTIFKFYFDAWILIGTGSLLTIGGWFAARRPILPARRWFAVAAAAALVV
                     APLALDIDIGRGLLGITHPPEGYHTLDGLASIDPGEAAAIDYLGTLGGGHRLVEAENG
                     DYGYYSRVSSFSGIPAVLGQISHELTWRGNGAWYTDRPADIRAIYENPGETLALMAKY
                     NATLLYVGEAERARYDVCLPGSGLVLIYEGDGVRIYAPEGVTIIPPG"
     gene            2717..3040
                     /locus_tag="PLI76_09055"
     CDS             2717..3040
                     /locus_tag="PLI76_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HOI62221.1"
                     /translation="MTPETATAEVAKSVTIDIVSVNMAFDLDTITVPAGAEVTINLDN
                     QDVGIPHNVSFYTDSSAAETIFVGDLITGPDKVTQTFTAPAEPGSYYFQCDVHPFMNG
                     TFVVE"
     gene            complement(3047..3940)
                     /locus_tag="PLI76_09060"
     CDS             complement(3047..3940)
                     /locus_tag="PLI76_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOI62222.1"
                     /translation="MRETFAALADLTRIHFFFVWPLLFCSGLALAFQNYGGFSWELVG
                     RAALIGLFGFEAGLVLNDYVDREYDRRDVDSSLTRYWRPFRARPIPEGKISPRAAFTL
                     FIALAGTAAALAATLPIPHNLYVLGIMGYSYLVEYFYQTKKRRQALPIAQVVGRTDFA
                     LFPVAGYLVIGSPDMTALFIFLFFYPWALAHLAANDIADVANDRARGMATIPVLYGMR
                     GAAAWVAGCAAAHAVTAVAFGLALGPVALAGFLAGLALLAGAACLILRGKSPAAAMQA
                     LPLIHATLIVYAVAIIATVAL"
     gene            4054..4515
                     /locus_tag="PLI76_09065"
     CDS             4054..4515
                     /locus_tag="PLI76_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62223.1"
                     /translation="MKNYWLIIAAVFAACCVGPASAQTAAEANASITVADQEVVGNGV
                     VGNITVEKVVSDGPGWVAIHNNLFGHPGGVIGYAPVKDGENTNVTVTVHTFVALDTLF
                     AVLHHDYGKEGVFEYPIPDVEQKTGGEIVIVPFNVTSRSATLMNLTELCPR"
     assembly_gap    4659..4758
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5048..5239
                     /locus_tag="PLI76_09070"
     CDS             5048..5239
                     /locus_tag="PLI76_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62224.1"
                     /translation="MEQSESPECTAATTLDRVGLKRRCRIVGVRAAGAFRKRVLAMGM
                     LPRWRPSRPPLSATPSSTG"
     gene            5304..5549
                     /locus_tag="PLI76_09075"
     CDS             5304..5549
                     /locus_tag="PLI76_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOI62225.1"
                     /translation="MMGGVYPFAFLVAGTEATIADIRGGREMRVRLSGMGLCPEARVT
                     IRSANQGSVIVAVGDARYALSRRIAVKILIRETQETV"
     gene            5546..>5875
                     /locus_tag="PLI76_09080"
     CDS             5546..>5875
                     /locus_tag="PLI76_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HOI62226.1"
                     /translation="MRKRVRIALTGNPNVGKTTLFNALTGSRQHVGNWPGVTVEKKTG
                     RINRNGYDIEIVDLPGTYSLTAYSADEIVARDFILEEKPDVVVQVVDATNLERNLYLT
                     TQLAELGA"
BASE COUNT          973 a         1901 c         1826 g         1075 t
ORIGIN      
        1 gctctccgaa ccctgctcgt ttcgggtata agagccgaaa accccgatct tgcggacgcg
       61 ataccgctcg cgaaggcagg atttcgcttt cctgagccga tcgatcgcac ggcccggtgc
      121 gccgggatat cctccggcct ttgcggcatg tcctggcctc cctgaagggg tatggggcga
      181 gagggcggtt taacgctttc ttcgcccggc atccccaaag tttatggatc tgcaggcgat
      241 tctctggagc ggtgagtgat ggtgaatatc tcccggctga agtacagggt cgaggtcctt
      301 ggcgaccacg acgacatcag gcgctacggt ccgttcaacg agcagaaggc acgggagttc
      361 ttcgaggtcg aggagagcct cggcggaacg gcccggatag cacggctgga ggaggggatc
      421 cgggagacgt ggcggatcgt tgccgaatgc ggggaatggg gatcacgaga agcggagttg
      481 ccggcccggt aacccctcgc cggccggttc ttcggcctcg gtgccgacct cccgccggtg
      541 gagaagcgta acctctcccc cgcggcccgg accctcgtcg ccatctgtag tgtggtcatg
      601 gcgccgccgg cgatcacaaa ccgcatatac cctccccgcg ataaatccgg gtgtggaccc
      661 cctcctgcag gcatcctctc tcctcctctg ggccggcctc ctgaaaggcc tccagctcgc
      721 cctctggccc cgcctccgcc ccgtgctcgg cgactacgcc tacccggcgg catacccggc
      781 ctccctcctc ctcttcgccc tcgcgacctg gtactgcggc ctcctccgta tcccggtcgc
      841 tctcgccctc ctcctctttg tcgccctcgg tatctacggc ctttaccggg gcgattaccg
      901 gggactccgg gggttctttg cctgggatgc ggtcttcctg gtcggctttc tcttcgctct
      961 ctctgtccgg ttcgcaaacc cggtcatcgg cagtttcagc gagcagtaca tgaaccatgc
     1021 cttccttgcg agcatcatcc gcgaacccat cgtgccgccg ctcgacccct ggtttgccgg
     1081 cgggcacctc accgtctact actacctcgg ccactggctg atgggctgcc tcgcggtcgt
     1141 caccggcacc ccctcccgga tcgccttcaa cctcatcccg gccacggtct acggggcggc
     1201 gttcgtcacg ctctacgccc tcgggcacct cttcctccgg cggctgcggt gggtgccgct
     1261 cgcggtcctc ctcctcgtcc cgccgtcggt tctctggttt cttgccgcgg gcggggatat
     1321 ctactccgcg ttccaggaga ccaactggat catatccggc gcccggtttg agttcccggc
     1381 cttctccctc ttcctcggga acgcccacgc cttcgagatg gccgtcttca accagatctt
     1441 cctcatattc ctcctcgggt ttgcgtggct ccggtggggc gggctcgata cccgggggcg
     1501 ggggggtctt gccctcctca tcgccctctc cctcggctcg atgccgctcc tctcctcctg
     1561 ggatgccctc gtctacggcc cggcggccgc ggtcttcctc cttgccctct tactccggga
     1621 gcgggaccgt tccaccgtag tcgccgccgt cgcggtcccg gcgatcgctc tcctcatcta
     1681 catcccctac tacctgcagc ttgaacccgc gggcgtgggc ggtatcggca tggggttccc
     1741 tcccaccgac cctctcgcgt tcatcgcgat ctggggcggg ttcctcgcgg tgatctacat
     1801 cgccgttgcc cgcgatatac ggcagtcccc gctctatctt ggtgtcgctc tcccgttcct
     1861 cgtcgccggc tacgccgccc tcggtatcgt cgcggtgccg ctcgcctacc tcatccgccg
     1921 gcgtcaacgc tcgtttgccg acatcctctc tcttgccggg cttctggtgc tcgccctctg
     1981 cgatgtcttc tacatcaagg agatgctcgg cggcgactac agccggttca acaccatatt
     2041 caagttctac ttcgatgcct ggatcctcat cgggaccggc tcgctcctca ccataggggg
     2101 atggtttgcc gcccgccgcc cgattctccc ggcgcggcgg tggtttgccg tcgccgcggc
     2161 cgcggcgctc gtcgtcgccc cgctcgccct cgatatcgac atcggccggg ggctcctcgg
     2221 gatcacccat cccccggaag gctaccacac cctcgacggg ctcgcctcca tcgaccccgg
     2281 ggaggccgcg gcgatcgact acctcggaac gctcggcggc ggccaccggc tcgtggaggc
     2341 ggagaacggg gattacggct actactcccg tgtctcgtcg ttctccggca tcccggcggt
     2401 cctcggccag atcagccacg aactgacctg gcgggggaac ggggcgtggt ataccgaccg
     2461 cccggcggac atccgggcga tctacgagaa cccaggagaa accctcgccc tgatggcgaa
     2521 gtacaatgcg accctcctct acgtcggtga ggcggaacgg gcgcgttacg acgtttgtct
     2581 gccggggagc gggctcgtcc tgatctacga gggggacggg gtccggatat acgcaccgga
     2641 aggcgtaacc attatacctc ccgggtgaca gggtggggct catgacgcga gcaactgcag
     2701 tcatggaggt gcgggcgtga ccccggagac ggctacggcc gaggtcgcga agagcgtgac
     2761 catcgatatc gtatccgtga acatggcgtt cgacctggat acgatcacgg tcccggcggg
     2821 tgcggaggtg accataaacc tcgacaacca ggatgtcggc ataccgcaca acgtctcgtt
     2881 ctacaccgac tcatcggcgg ccgagacgat cttcgtcggc gacctcatca ccggcccgga
     2941 caaggtgacc cagacattca cggcgccggc ggagccgggg tcctactact tccagtgcga
     3001 tgtccacccg ttcatgaacg ggacgttcgt cgtggagtag gatccatcag agcgcgacgg
     3061 tcgcgatgat cgctacggcg tagacgatca gggtcgcgtg gatgagcggg agcgcctgca
     3121 tcgcggcagc cgggcttttc ccccggagga tgaggcaggc tgcgcctgca aggagggcga
     3181 gccccgccag gaatccggcg agggccaccg gtccgagggc gaggccgaag gcgaccgccg
     3241 tcacggcatg ggcggccgca cacccggcga cccaggcggc cgcacctctc atgccgtaga
     3301 ggaccgggat cgtcgccatc ccccgggcgc ggtcgtttgc gacgtcggcg atgtcgtttg
     3361 ccgcgaggtg cgcgagcgcc caggggtaga agaagaggaa gatgaagaga gcggtcatgt
     3421 cggggctgcc gataacgagg tagccggcca cgggaaagag ggcgaagtcc gtccgcccga
     3481 ccacctgcgc gatcgggaga gcctggcgcc gcttttttgt ctggtagaag tactcgacca
     3541 ggtaggagta gcccatgatg ccgaggacgt aaaggttgtg cgggataggg agggtcgcgg
     3601 cgagggcggc cgcggttccg gcgagggcga tgaagagggt gaacgccgcc cggggcgata
     3661 tcttcccctc cggtatcggc cgcgcccgaa acggccgcca gtagcgggtg agcgagctat
     3721 cgacgtcccg gcggtcgtac tcccggtcga cgtagtcgtt caagacgaga ccggcctcga
     3781 acccgaagag gccgatgagc gccgcacggc cgacgagttc ccacgagaat ccgccgtagt
     3841 tctggaacgc gagtgcgagc cccgagcaga aaagcagcgg ccagacgaag aagaagtgaa
     3901 tacgggtcag atcggcgagc gctgcgaagg tctctctcat ggggtgtatg atggggtata
     3961 cggccgcacc caaaaaaagg ttgcagatct cacccgaacc ctttatgtat acccgtggca
     4021 tctaccccct cccccacggg ggaggtcaat ggcatgaaga actactggtt gatcattgca
     4081 gcggtcttcg cggcctgctg tgtcggtccc gcctccgccc agaccgctgc cgaggcaaat
     4141 gcctcgatca cggtggcgga ccaggaggtc gtgggcaacg gggtcgtcgg gaacataacg
     4201 gtcgagaagg tcgtgagcga cggccccgga tgggtcgcca tccataacaa cctcttcggg
     4261 catcccggcg gcgtcatcgg gtatgctccg gtaaaggatg gagagaatac gaacgtcacg
     4321 gtcacggtcc acacgttcgt cgccctcgat accctgtttg cggttttgca ccacgattac
     4381 gggaaggagg gcgtctttga gtaccccatc cccgacgtcg agcagaagac agggggcgag
     4441 atcgttatcg tgcccttcaa cgtaacatcc aggagcgcca ccctgatgaa cctgaccgag
     4501 ctctgcccgc ggtgagggaa ctgacgggac ggtcaatcgc tctcaacttt tttttcagga
     4561 tatcttttca aatccgaaac cgtttcgggt gaatctcatc cctcccgaag gccttaccgg
     4621 gtatgtagca accatggaag acacaggaaa cgatacgcnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnntt ggcgggtgtt ccgggatgag cagcggtgcg tcgatccgcg
     4801 gcacccggga tccggaggcc gtccgggagt tcatccaaca accattcgga tcggatccgg
     4861 agaggcggga agagcgccgg cccgggtagc ccgtgcttcc gcctgctacc gtggagatcc
     4921 cgggaagccg ttcatgccca tcgaccttcg gattttctcc ttttccgaaa aaggttcgtt
     4981 tcagagactc atatcctgac atttttcggc ccgggattat atcgaccaaa cgagagaaaa
     5041 aggtcgtatg gaacagtcag aatcaccaga atgcacggcg gcgaccacgc tcgatcgtgt
     5101 cggcttgaaa cgccggtgcc gtatcgtcgg tgtccgcgcc gccggggcgt tcaggaagcg
     5161 cgtgctcgcc atgggtatgc tccccaggtg gagaccctcg aggccgcccc tctcggcgac
     5221 cccatcgagt accgggtgaa agggtactgc ctctcgctac ggcgcgcctc agcgcgcctc
     5281 atcgaggttg tcccggagga gggatgatgg gcggtgtcta tcccttcgcg ttccttgtcg
     5341 ccggaaccga ggcaacgatc gccgatatcc ggggcggcag agagatgcgg gtgcgcctct
     5401 ccgggatggg gctctgcccc gaggcccggg tgacgatccg gtctgcaaac cagggttcgg
     5461 tcattgtcgc ggtcggcgac gcccggtatg ccctctcgcg caggatagcg gtgaagatac
     5521 tcatcaggga gacgcaggag accgtatgag gaagagggtc cggattgcgc tcaccggcaa
     5581 tcctaacgtc ggcaagacaa cgctcttcaa cgcgctcacc ggctcaaggc agcacgtcgg
     5641 gaactggccc ggggtgacgg tggagaagaa gaccggccgg atcaaccgta acggctacga
     5701 tatcgagatc gtcgatctcc ccggaaccta cagcctgacc gcgtactctg cagacgagat
     5761 cgttgcgcgg gacttcatcc tcgaagagaa gcccgacgtc gtcgttcagg tcgtggatgc
     5821 gacgaacctg gagcggaacc tctacctgac gacccagctc gccgaactcg gggcc
//
LOCUS       DAOSEE010000129         5792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_4942_length_5792_cov_220.264598, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000129 DAOSEE010000000
VERSION     DAOSEE010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4942_length_5792_cov_220.264598"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..1434)
                     /gene="thiC"
                     /locus_tag="PLI76_09085"
     CDS             complement(160..1434)
                     /gene="thiC"
                     /locus_tag="PLI76_09085"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOI62227.1"
                     /translation="MGLIEDARRGVVTEEMRIVAAAEGVTEDFIRRGVADGHIVIPVS
                     PYRRVKFCGIGEGLRTKVNASIGTSSDIIDVDMEIEKARQAERAGADTLMELSTGGDF
                     TEIRRRVVEATTLSVGSVPLYQAFIEAARKHGAVVHMEEDDLFRITAEQAKLGTNFMA
                     IHTGINYETMKRLQNQGRHGGLVSRGGAFMTAWMIHNNKENPLYSEFDYLLEILKEHE
                     VTLSFGNGMRAGAVHDATDRAQVQELLINAELADKAHAEGVQAIIEGPGHIPIDEIQT
                     NVVLQKRVTNRKPFYMLGPLVTDIAPGYDDRVAAIGAALSSSYGADFICYVTPAEHLA
                     LPTPEEVYEGVMSSRIAAHVGDMIKLRKRDADLEMGHARRDLDWERQFAVAMNPERAR
                     AIRAERMPADADACTMCGDYCALKIVGRHFNF"
     gene            1613..2323
                     /locus_tag="PLI76_09090"
     CDS             1613..2323
                     /locus_tag="PLI76_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOI62228.1"
                     /translation="MYVNFGGFVPISTVDWRGRAACTVFLRGCPARCFYCHNVALQGG
                     EDLRPVDEVVAMIRSSRPVAGAVVFSGGEPTLQGPALIRLAAAARGMGLSVGLHTNGI
                     FPGVIEALLRERLVDMIALDIKTTWERYDDLIELTAADAVKRSLALCKEAKADGSLGT
                     CEIVTTLFRGREGDLPTIAEAARGLDLVLQQGVTAGYVPLSRQEIETAAAPLGQKVRI
                     RTREDGEVGYDPEQNTSP"
     gene            2449..2997
                     /locus_tag="PLI76_09095"
     CDS             2449..2997
                     /locus_tag="PLI76_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOI62229.1"
                     /translation="MKVIGIVGMPASGKGEVSRIARDLGIPVVVMGDAIREKVKEAGL
                     PLTDANLGAISGKLRADLGMDAIARITIPVIEATAAPVVLVDGIRGDYEVTAFREHFP
                     DFTLIAIVSSFETRYARLANRGRSDDSLTAGELRSRDERELGWGLLRALAQADCRITN
                     EGTLEEFAAEARSLLCRVGGLS"
     gene            2994..3767
                     /locus_tag="PLI76_09100"
     CDS             2994..3767
                     /locus_tag="PLI76_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOI62230.1"
                     /translation="MSLTPYFSSSSKVWESRDWIFGLEEIGYVGWEIVADGKYRLDNP
                     ENFIAIQENLASTGLRSTVHAPYSDLNLASLNYPIWRESIRQTCCCIHHAADLTDRVT
                     IHPGFLSPVGKLVPEKVWEMQKTALVEIGKHAEDHGVLACVENMISIKDFLCRYPEEI
                     LGLTEGIAGIGVTLDIGHANTNGLADAFLAHVGEVNHLHIHDNHGQSDEHLALGDGTI
                     NWEKVGRIVARDYSGPVVIEGRTLEEAGRSLAVFRKWFV"
     gene            3755..4708
                     /locus_tag="PLI76_09105"
     CDS             3755..4708
                     /locus_tag="PLI76_09105"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOI62231.1"
                     /translation="MVRIAGETLHVHFLGTAGSLPSPQRNPPCILIRRGSDTLLFDCG
                     EGAQQQMMRAKTGFTIDAIFVTHWHADHFLGIFGIVETLGFMERTAPLPIYGPPWVVE
                     FVDLIQRINRHTRGFAVTAHPLDHGSVVPFNGYTVRAFATIHGIPGLGYVLEEDERPG
                     RFNRDHAIALGVPPGPLFGRLQRGETVRIVRDGVETEVRPGDVLGEPRPGRKVVYTGD
                     TRPIQNQPGVAALIRGADLLIHDATFDDQESSRAREVLHATAGEAGEAAAALNARMLA
                     LVHISSRYASTANHIRDAKRQYEGDVILPADLTALEIPFRS"
     gene            4724..>5792
                     /locus_tag="PLI76_09110"
     CDS             4724..>5792
                     /locus_tag="PLI76_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOI62232.1"
                     /translation="MVMEQGLCSLSRRRLILTGLGALLVSAAAASVAGIYLGAAREVL
                     MLIPGLMVLLPSIIDMRGNIAGVLASRLSSSMHLGVFSIDFEEGSVLGDNIRASFVIT
                     VLIAFVLGIFAYLASLLSGLPTVGVTDLVLISVTSGIVSGLVVMGITVVIALASYRYG
                     LDLDMIAAPTVTTSGDIVTLPILMLSAVFVINLPPWARLTLGIAALAAAGAAILYTRH
                     RPVGIGTIVRENLALLIPLSVLGTLAGLTYSLNLDDLVTFAAFLILIPPFMGGLGSIG
                     GILGSCLSTGMHTGELAPTLIPRRDVVHHFIISYLYTLILLPLLALIAHGAAVLMGLN
                     SPGLGILVTISLIAGLIVMTLV"
BASE COUNT         1004 a         1899 c         1792 g         1097 t
ORIGIN      
        1 agccggcggg cagccgtgct ccggagcgac gccggcgtgc gccgcctccc cgggtggatg
       61 cccgtatgct cttcgctaaa tagcgccgga tgcttgcttg cacaccagaa agccgttcta
      121 taaaaaccgg cgggagccgg gggaagatcc ccgcctccgt cagaagttaa agtgccgccc
      181 gacgatcttc aacgcacagt agtccccgca catcgtgcag gcgtcggcgt cggccggcat
      241 ccgctcggcc cggatcgccc gggcgcgctc ggggttcatc gcgacagcaa actgccgctc
      301 ccagtcgagg tcgcggcggg cgtggcccat ctcgaggtcg gcgtcccgct tcctgagctt
      361 gatcatatcc ccgacgtggg cggcgatccg cgaactcatg acgccctcgt agacctcttc
      421 gggcgtgggg agcgccaggt gctccgccgg cgtcacgtag cagatgaagt cggcgccgta
      481 agaggaggag agcgcggccc cgatcgcggc cacccggtcg tcgtagccgg gcgcgatatc
      541 ggtgacgagc ggcccgagca tgtagaacgg ttttctgttc gtgacccgct tctgcaggac
      601 gacgttcgtc tggatctcat cgatcgggat atgccccggc ccctcgatga tcgcctgcac
      661 gccctctgcg tgtgccttat cggcaagttc cgcgttgatg agcagctcct gtacctgggc
      721 acggtcggtc gcatcgtgga ccgcccccgc ccgcataccg ttgccgaacg agagggtgac
      781 ctcgtgctct tttaagatct cgagcaggta gtcgaactcc gaatacagcg ggttctcctt
      841 attattgtgg atcatccacg cggtcatgaa cgccccgccc cgggagacga gcccgccgtg
      901 ccggccctgg ttctggaggc gcttcatcgt ctcgtagttg atcccggtgt ggatcgccat
      961 gaagttggtc ccgagtttcg cctgctccgc cgtgatccgg aagagatcgt cctcctccat
     1021 gtgaacgacc gccccgtgtt tccgggcggc ctcgatgaac gcctggtaga gcggaaccga
     1081 cccgaccgag agggtggtcg cctcgacgac ccggcgccgg atctccgtaa agtcaccgcc
     1141 ggtggagagc tccatcaatg tatcggcgcc ggcacgctcg gcctgccgcg ctttctcgat
     1201 ctccatatcg acgtcgatga tatccgacga tgtcccgatg ctcgcgttga ccttcgtgcg
     1261 gagcccctcg ccgataccgc agaacttaac tctccggtaa ggggataccg gtatgacgat
     1321 gtggccgtcg gccacgccgc gcctgatgaa atcctcggtt acgccttctg ctgctgcgac
     1381 gatccgcatc tcctcggtga cgacaccccg ccgggcgtct tctatgagac ccatgacggt
     1441 atatttggaa gaaagagatg ataaaggctc gttttttacg aatcagacta agatatggtt
     1501 gtttcctcat caagtaaaat tgacttaccc ttattcaggt ccggtttcgt agccggaaag
     1561 ccaaggctta caagcgatcg gggagcacca gtacaccgtc cggtgaacag cattgtacgt
     1621 caatttcggg ggattcgtcc ccatcagcac cgtcgactgg cggggacggg cggcctgcac
     1681 ggtcttcctc cgggggtgcc ccgcccgctg cttctactgc cataacgtcg ccctccaggg
     1741 cggagaggac cttcgccccg tagatgaggt cgtcgcgatg atccggagct cccgcccggt
     1801 tgccggcgcc gtcgtcttct ccggcggaga accgaccctg cagggtccgg cgcttatacg
     1861 tctcgccgcc gccgcccgcg gcatggggct ctctgtcggc ctgcacacaa acggcatctt
     1921 tccaggagtg atcgaggccc tcctccgcga acgcctggtg gacatgatcg cgctcgacat
     1981 caagaccacg tgggagcgtt acgacgacct tatcgagctt accgccgctg atgccgtgaa
     2041 acggtcgctc gccctctgta aagaggcaaa agccgacggc agcctcggga cctgtgagat
     2101 cgtgaccacc ctcttccgtg gccgcgaagg cgacctcccg acgattgcag aggccgcccg
     2161 cgggctcgac ctcgtcctcc agcagggggt gaccgcaggg tacgtgcccc tctcccggca
     2221 ggagatcgag acggcggcgg cacctctcgg gcaaaaggtc cggatacgga caagggagga
     2281 cggcgaggtc ggatacgacc cggagcagaa cacctccccg tgacgttaag catattatcg
     2341 tcgggagcgc aaggttcatg gatatggttg tggcggccgc cacgagccag gagtccggag
     2401 caccggcacg gttgttctcg atagagcacg atacaacgga gaaacctgat gaaggtcata
     2461 ggaatcgtcg gtatgccggc aagtggaaaa ggagaggtat cgaggatcgc ccgggacctc
     2521 gggataccgg tcgtggtcat gggcgacgcg atacgggaga aggtgaagga ggccgggctt
     2581 cccctgacgg acgcaaacct cggcgcgatc tcggggaagt tgcgcgcaga cctcggtatg
     2641 gatgctatcg cccggatcac catcccggtc atcgaggcaa cggctgctcc ggtggtcctc
     2701 gtggacggca tcaggggaga ctacgaggtg acggcgtttc gggagcattt ccccgacttc
     2761 acgctcatcg cgatcgtctc gtcgtttgag acgcgctacg cgaggcttgc gaaccggggg
     2821 cggtcggacg actccctcac cgccggagaa ctccggtccc gcgacgagcg ggagctcggg
     2881 tgggggcttc tgcgggctct cgcgcaggcg gactgccgga tcacgaacga aggcacgctc
     2941 gaagagtttg ctgcagaggc ccggagcctg ctctgccgcg tgggagggct ctcatgagcc
     3001 ttaccccgta cttctcctcc tcatcgaagg tctgggagtc gcgcgactgg atcttcggcc
     3061 tcgaagagat cgggtacgtc gggtgggaga tcgtcgccga cgggaagtac cgcctggaca
     3121 accccgagaa ctttatcgct atccaggaga acctcgcgag caccggcctt cgctcgaccg
     3181 ttcacgctcc ctacagcgac ttgaacctcg cctcgttgaa ctaccccatc tggcgcgagt
     3241 ccatccgcca gacctgctgc tgcatccacc acgcggccga cctgaccgac cgggtgacga
     3301 tccacccggg tttcctctcc cctgtgggaa aactcgtccc cgagaaggtc tgggagatgc
     3361 agaagaccgc actcgtcgag atcgggaagc atgcggaaga tcacggcgtg cttgcatgcg
     3421 tcgagaacat gatcagcatc aaggacttcc tctgccgcta ccccgaggag atcctcggac
     3481 tcacggaggg gatagcgggg ataggggtca cgctcgatat cgggcacgcg aacaccaacg
     3541 gccttgccga tgcattcctc gcgcatgtcg gggaggtcaa tcacctccac atccacgaca
     3601 accacgggca gtcagacgag cacctggcac ttggtgatgg aaccatcaac tgggagaagg
     3661 tcgggcgcat cgtcgcccgc gactactccg gcccggtcgt gatcgagggg cgaacgcttg
     3721 aagaggcggg acggagcctt gccgtcttca ggaagtggtt cgtatagccg gcgagaccct
     3781 gcacgttcac ttcctcggca cggccggatc gcttccgtca ccgcagagga accccccctg
     3841 catactgatc cggcggggct ccgacaccct gctcttcgac tgcggggagg gcgctcagca
     3901 gcagatgatg cgtgcgaaga ccgggttcac catcgatgcc atcttcgtca cgcactggca
     3961 cgccgatcac ttcctcggca tcttcgggat cgtcgagacg ctcgggttca tggagcgcac
     4021 cgccccgctc ccgatctacg gcccgccctg ggtcgtggag ttcgtcgacc tcatccagcg
     4081 gatcaaccgc cacacccggg ggttcgccgt cacggctcat cccctcgatc atgggtcggt
     4141 cgtgcccttt aacggctaca ccgtcagggc gtttgcgacg atccacggca tccccggtct
     4201 cggttacgtt ctggaggagg acgagcggcc ggggaggttc aaccgtgacc acgcgatcgc
     4261 gctcggcgtc ccgccgggcc cgctcttcgg tcggctccag cgcggggaga cggttcgcat
     4321 cgtccgcgac ggcgtcgaga ccgaggttcg gcccggcgac gtgctgggcg agccgcgtcc
     4381 ggggaggaag gtcgtctaca ccggagatac gcggcccatc cagaatcaac ccggggtcgc
     4441 cgcgctgatc cggggcgccg acctcctgat ccacgacgcc acgttcgacg accaggagag
     4501 cagccgcgca cgagaagtcc tgcatgccac cgccggcgag gccggcgagg cagcggcggc
     4561 cttaaacgct cgcatgcttg ccctggtgca catcagctcc cgctatgcaa gcacagcaaa
     4621 ccatatacgc gatgcaaagc gacagtatga aggagacgtg atcctcccgg ccgatctcac
     4681 ggccctggag atcccttttc ggagttgatg ataatggacg gtgatggtga tggagcaggg
     4741 tctatgcagc ctgagccgcc gccgtcttat cctgaccggt ctaggcgccc tgctcgtcag
     4801 cgctgccgcg gcgagcgttg cgggcatcta tcttggcgcc gcacgtgaag tcctcatgct
     4861 gatacccggc ctgatggtgc tcctgccgtc gatcatcgat atgcggggca acatcgccgg
     4921 ggttctcgca tcccgcctct cgtcgtcgat gcacctcggg gtcttctcca tcgactttga
     4981 ggaggggtcc gttctcggcg acaacattcg cgcttcgttt gtcataaccg tcctcatagc
     5041 cttcgttctc ggcatattcg cctacctggc aagccttttg tccggcctcc cgacggtggg
     5101 cgtcaccgat ctcgtgctga tctcggtcac gagcgggatc gtctcggggc tcgtggtgat
     5161 ggggatcacc gtggtcatcg cgcttgcgag ttaccgctac ggcctcgacc tcgacatgat
     5221 cgccgccccg accgtcacca catccgggga tatcgtcacg ctcccgatcc tcatgctctc
     5281 cgcggtcttc gtcatcaacc tcccaccctg ggcgcgcctg acgctcggga ttgccgcgct
     5341 cgccgcggcc ggtgccgcca tcctctacac ccggcaccgg ccggtgggaa tcgggacgat
     5401 cgtcagagag aacctcgctc tcctcatacc gctcagcgtc cttgggacgc tggccggatt
     5461 gacctactcc ctcaacctcg acgatctggt gacgtttgcc gcgttcctca tcctgatacc
     5521 gccgtttatg ggcggtctcg gctcgatcgg cggcatactc gggtcatgcc tctcgaccgg
     5581 gatgcacacc ggcgaacttg ccccgactct catcccccgg cgcgatgtcg tgcatcactt
     5641 tattatttcc tacctctata cgttgatact gcttccattg ctggcgctca tagcacatgg
     5701 cgcggcggtg ctcatggggt taaacagccc cggccttgga atactggtca ctatcagcct
     5761 tatcgcgggt ctgatcgtca tgaccctggt ga
//
LOCUS       DAOSEE010000130         5740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_5010_length_5740_cov_233.316799, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000130 DAOSEE010000000
VERSION     DAOSEE010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5010_length_5740_cov_233.316799"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /gene="endA"
                     /locus_tag="PLI76_09115"
     CDS             <1..588
                     /gene="endA"
                     /locus_tag="PLI76_09115"
                     /EC_number="4.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000214 - tRNA-intron endonuclease
                     complex [Evidence IEA];
                     GO_function: GO:0000213 - tRNA-intron endonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006388 - tRNA splicing, via
                     endonucleolytic cleavage and ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-intron lyase"
                     /protein_id="HOI62233.1"
                     /translation="AVVDDEDELTYYEVRVQDLPGVGEPARCDRPIEASLFGTYALAH
                     LPPGTPLEEGWYGKRLDPERLLFRPVESIYLMQKGCLAVTRDGGRMTAEEFRGVAAEK
                     DIEIREKERVFSDLREKGYIPRTGYKFGHHFRVYSGKKTHSEMLVHAVPSRTSTPMSA
                     VSRSVRLAHSVKKKMLFACIYNSDIGYIEFARIKL"
     gene            599..1855
                     /locus_tag="PLI76_09120"
     CDS             599..1855
                     /locus_tag="PLI76_09120"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOI62234.1"
                     /translation="MQSGMNPWSSNQTVDVDRLFAEFGIEPVGEVARGLPEVPPFMRR
                     GVVVGHRDYGLIADAIRNRTPFHVLTGFMPSGLPHLGHLMVMKEVVWHVRQGGNGYVA
                     IADREAHAVRGISWEKCREYGKEYLKALYALGFCGTTYYQSRNDRLKDLAFEASTKVN
                     FSELTAIYGFGPETSLSHAVSVVTQVADILFPQLDAGPAPTVVPVGLDQDPHIRLTRD
                     VAYKLRMFTVEDRGDHISVRSKNAPEAALEAVHRTFSGSKKYAGHVDVTGVPMARIED
                     AVRGIEIARGGFGFYLPSSTYHIFMPGLQGGKMSSSVPESLFGFYEPEPSVRKKIMGA
                     LTGGRMTLEEQRRLGGEPGGCPVFLLNLFHMLEDDRELADLRRRCEGGELTCGQCKKE
                     TYERVQAFLRELRDKMDAVEHLAEEV"
     gene            1859..3304
                     /locus_tag="PLI76_09125"
     CDS             1859..3304
                     /locus_tag="PLI76_09125"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOI62235.1"
                     /translation="MELTQNEKRLLAALGPVGSADAAALAGMMDARREAVVQYANLAG
                     ERGLVDVEKRISRRYVPTDEGRACAGKGLPERQVFESFGEAIPMRDLQAHPLAKIAVG
                     WMRKKGWVTIRDGVVQKTGNAAPGPDETVFARISENGSAEDGPGVADLLRRGLVEEEE
                     TVTYTVSITPLGCSLLAGGLDLEEEAGTLTRDQILSGAWRDLPLRRYDVTKLPRRAYP
                     GKVHPYQRLLDEMRRILLDMGFTEMAGGIVQSSFWNFDALFQPQDHPAREMQDTFFLG
                     ERWPLPAGYERVRDMHEHGGETSSAGWGGTWSAAKAEQCVLRTHTTGLSIQHLAKHPR
                     PPVRAFCIGRVYRREAVDPTHLAEFEQLEGIVMDEGVNFRHLLGFLKEFYAKMGFEKV
                     RFRPGYFPYTEPSVEPEVYVENLGWVELGGAGIFRQEVTAPFGIEHPVLAWGLGISRV
                     AMLRLGLRDLRHLYRSDVEWIRETPTYGGRR"
     gene            3306..4946
                     /gene="pheT"
                     /locus_tag="PLI76_09130"
     CDS             3306..4946
                     /gene="pheT"
                     /locus_tag="PLI76_09130"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOI62236.1"
                     /translation="MAIITLPYRYLERLTGTDRQTIIDRVPMIGADIERIEEDHVDVE
                     FFPDRPDLYSPEGVARAMRGFLGIEEGLPVYTVHPSGITFSVDPGLAGIRPHLASAVI
                     RNVDLDEEAIESLMSLQEALHWAVGRGRAKVAIGVHDLDMVTPPFRYIASPRSRSFVP
                     LDFDREMTMEEILVDHPKGRDYARLVEDFPRFPLIVDAEDRVLSFPPIINGELTRVTA
                     ATKNILLDCTGTDRKAVMTATNIICTALAEAGATIESVTVAGEEMPTLAPAERIVSVD
                     ECAALLGIPLTGEEMARLLRRMRFGAEPEGASHLRVFVPCYRSDILHDWDIFEDAAIA
                     YGFENFDAALPPTSTTAEEHPVTAIAGAVRSVMIGLGYLEMMPFTLTNERVLYANMQR
                     EPLPGTLRVLHPISEEQTVVRTDLLPLLMEMLQANKHRELPQRLFAVGDVVRYCITSQ
                     MAAAVSIHPAADFSEAYAAADVLCRELALPYTVAESSDPAFIDGRRGDIIVDGKIVGV
                     FGEIHPAALNAFDLEHSVAAFALDLTAVPGYPVPPGTP"
     gene            complement(4903..5709)
                     /gene="hisF"
                     /locus_tag="PLI76_09135"
     CDS             complement(4903..5709)
                     /gene="hisF"
                     /locus_tag="PLI76_09135"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOI62237.1"
                     /translation="MVPTKRIIPCLDLKDGRVVKGTHFTDLCDAGDPVALARRYDEQG
                     ADELVFLDIAASKEHRGTIIDVIRRASDQLALPFTVGGGIRTIDDMQQILGAGADKVS
                     INSSAVENPELISRGAERFGAQAIVVAIDVRRNYAVEPGKKPIALADGRECWYEVVTH
                     GGSRATGRDAVAWAREAEERGAGEILLTSMETDGTKDGFDIPITAAVATAVGIPVVAS
                     GGVGTLEHFYEGFAEGKADACLAASVFHYGVFAIREVKEYLAGRGIPVRR"
BASE COUNT          997 a         1878 c         1863 g         1002 t
ORIGIN      
        1 gcggtcgtgg acgacgagga cgaactgacc tactacgagg tgcgggtgca ggacctcccc
       61 ggcgtcgggg agccggcccg gtgcgacaga ccgatcgagg cctcgctctt cggcacctac
      121 gcgcttgccc acctgcctcc gggaaccccg cttgaggagg gctggtacgg caaacggctt
      181 gaccccgaga ggctcctctt ccgcccggtc gagtcgatct acctcatgca gaaggggtgc
      241 ctcgcggtca cgcgggatgg gggtcggatg accgcggaag agttccgcgg tgttgcagcc
      301 gaaaaagaca tcgagatccg cgagaaagag cgggtcttct ccgatctccg ggagaaaggc
      361 tacataccga ggaccgggta caagttcggc caccacttcc gggtctactc cggaaagaag
      421 acccactccg agatgctcgt ccacgccgtc ccttccagaa cctccacgcc gatgagcgct
      481 gtctcccggt cggtcaggct tgctcacagc gtcaaaaaaa agatgttgtt tgcctgcata
      541 tacaatagcg atatcggata catcgaattt gcccgaatca agctgtgagc gtcttttcat
      601 gcagtccgga atgaatccgt ggtcaagtaa tcagaccgtc gatgtagatc gactctttgc
      661 cgagttcggc atcgagcctg tcggtgaggt cgcacgaggg cttcccgagg taccgccgtt
      721 tatgcgcagg ggtgtcgtcg tcggacaccg ggattacggc ctgatagccg atgccatccg
      781 gaaccgcacc ccgtttcacg ttctgaccgg gttcatgcct tcagggctcc cgcacctcgg
      841 acacctgatg gtcatgaagg aggtggtctg gcacgtccgg cagggcggga acggctacgt
      901 ggccatcgcc gaccgcgagg cgcacgcggt ccgcggcata tcgtgggaga agtgccgcga
      961 gtacgggaag gagtacctca aagccctcta cgccctcggg ttttgcggca cgacctacta
     1021 ccagagccga aacgatcggt taaaagacct cgcgttcgag gcgtccacga aggtgaactt
     1081 ctccgagctt acggcgatat acgggttcgg gcccgagacg tcgctctcgc atgccgtgag
     1141 cgtcgtcacc caggtggccg atatcctctt cccgcagctc gacgccggcc ccgcccccac
     1201 ggtcgtcccg gtcggcctcg accaggaccc gcacatccgg ctcacccgtg acgtggccta
     1261 caagctcagg atgttcacgg tcgaggaccg cggcgaccat atcagcgtcc ggtcgaagaa
     1321 tgccccggag gcggctctcg aggccgtaca ccgcacgttc agcggctcaa agaagtacgc
     1381 gggccacgtg gacgtcaccg gcgtgccgat ggcgcgcatc gaggatgccg tccgcgggat
     1441 agagattgcc cgcgggggat tcgggttcta tctcccctcg tcgacctacc acatattcat
     1501 gcccggcctc cagggcggga agatgtcgag cagcgtgccc gagagccttt ttgggttcta
     1561 cgagcccgaa ccgtcggtca gaaagaagat catgggcgcg ctcaccggcg ggcggatgac
     1621 gctcgaggag cagaggcgcc tcggagggga gcccggcggc tgcccggtct tcctcttaaa
     1681 tctcttccac atgctcgagg acgatcggga acttgccgat ctccgccgcc ggtgcgaggg
     1741 aggcgagctc acctgcgggc agtgcaagaa ggagacatat gaacgcgtgc aggcgttcct
     1801 tcgtgagttg cgggataaga tggatgcagt agagcacctt gcagaggagg tgtgaacggt
     1861 ggaactgaca cagaacgaga agaggctcct ggctgccctg gggccggtgg gatcggccga
     1921 tgcggccgcc cttgccggca tgatggatgc ccgccgggag gcggtggtgc agtatgcaaa
     1981 ccttgccggc gagcggggac tggtggatgt cgagaagcgc atctcccgac ggtatgttcc
     2041 gaccgacgag gggcgggcct gcgccgggaa gggccttccc gagcggcagg tgtttgagag
     2101 tttcggagag gccatcccga tgcgggacct gcaggcgcac ccgcttgcaa agatagccgt
     2161 cggctggatg cggaagaagg gctgggtcac catacgggat ggggtcgtgc agaagaccgg
     2221 caacgccgct cccggacccg acgagaccgt gtttgcccga atcagcgaga acggttcggc
     2281 tgaagatggc cccggggttg ccgaccttct ccggcgcggg ctcgtggagg aggaggagac
     2341 cgtcacctac accgtctcga tcacgcctct tggctgcagc ctcctcgccg gagggcttga
     2401 cctcgaggaa gaggcgggta cactcacccg cgaccagatt ctctccggtg catggaggga
     2461 tctccccctc cggcgctacg acgtgacgaa acttccccgg cgcgcctacc ccgggaaggt
     2521 ccacccctac cagcgccttc tcgacgagat gcgccgtatc ctccttgaca tggggttcac
     2581 cgagatggcg ggcggtatcg tccagagttc gttctggaac ttcgacgccc tcttccagcc
     2641 gcaggatcac ccggcgcggg agatgcagga caccttcttc ctcggcgagc gctggccgct
     2701 cccggcgggg tatgagcgcg tccgcgatat gcacgagcac gggggcgaga catcttccgc
     2761 cggctggggg ggaacctgga gcgccgcaaa ggcagaacag tgcgtgctcc ggacccatac
     2821 gacgggcctc tccatccagc acctcgcgaa gcacccgaga ccgcccgtac gggcgttctg
     2881 catcggccgg gtctaccggc gggaggcggt cgaccccacc caccttgcgg agttcgagca
     2941 gctcgagggg atcgtcatgg acgaaggcgt caacttccgc cacctcctcg ggttcttaaa
     3001 ggagttttac gccaagatgg gctttgagaa ggtcaggttc cgccccggct acttccccta
     3061 caccgagccc tccgtggagc ccgaggtcta cgtcgagaat ctcggctggg tggagctcgg
     3121 cggagcgggc atattccggc aggaagtgac cgcgcccttc ggcatcgagc acccggtcct
     3181 cgcgtggggt cttggtatca gccgggtggc gatgctccgt ctcggcctta gggacctccg
     3241 gcatctctac aggagcgacg tcgagtggat cagggagacg cccacctacg gcgggaggcg
     3301 gtgatatggc tataatcacg ttaccctacc ggtacctgga gcgattgacc gggaccgacc
     3361 ggcagacgat catcgaccgc gttccgatga tcggggccga tatcgagcgg atcgaggaag
     3421 accacgtgga cgtcgagttc ttcccggacc gccccgacct ctactctccc gagggtgttg
     3481 cccgggcgat gcggggattc ctcggcatcg aggaggggtt gccggtctac accgtccacc
     3541 cgtccggcat aacgttctcg gtcgacccgg gccttgccgg catccggccg caccttgcct
     3601 cagcggtgat ccggaacgtc gacctcgacg aggaggcgat cgagagcctg atgagccttc
     3661 aggaggcact ccactgggcg gtcgggcgcg ggcgggccaa ggtggcgatc ggcgtccacg
     3721 acctcgatat ggtcacgccg cccttccgct acatcgcctc tccccggagc cggtctttcg
     3781 tcccgctgga cttcgaccgg gagatgacga tggaggagat cctcgtcgac cacccgaagg
     3841 gccgcgacta cgcccgcctc gtggaggatt tcccccggtt cccgctgatc gtcgatgcgg
     3901 aagaccgggt gctctcgttc ccccccatca tcaacggcga gttgacacgg gtcacggcgg
     3961 cgacgaagaa tatcctgctc gactgcaccg gcaccgaccg aaaagcggtg atgacggcga
     4021 cgaacatcat ctgcaccgcg cttgccgagg ccggggcgac gatcgagagc gtcaccgtcg
     4081 caggcgagga gatgccgacg cttgcccccg cggagcgtat cgtctcggtc gatgagtgcg
     4141 ccgctctcct cggcatcccc ctcacggggg aggagatggc gcggctcctt cgccggatgc
     4201 ggtttggggc cgaaccggag ggcgcgtccc acctccgggt cttcgtcccg tgttaccgct
     4261 cggacatcct ccacgactgg gatatattcg aggacgcggc gatcgcctac ggtttcgaga
     4321 acttcgacgc cgccctcccg ccgacgtcca cgaccgccga ggagcacccg gtcaccgcca
     4381 tagcgggagc ggtccggtcg gtgatgatcg gccttgggta cctcgagatg atgcccttca
     4441 ccctcacgaa cgagcgggtg ctctacgcga acatgcagcg cgagcccctg cccgggaccc
     4501 tccgggtgct ccacccgatc agcgaggagc agaccgttgt ccggaccgac ctccttcccc
     4561 tgctcatgga gatgctccag gcgaacaaac atcgcgagct cccgcaacgc ctctttgcgg
     4621 tgggcgacgt ggtcaggtac tgtatcacct cccagatggc cgcggcggtg agcatccatc
     4681 cggctgccga cttctctgag gcatacgcgg cggccgacgt cctctgccgg gagctcgcgc
     4741 ttccctatac ggtcgcggag tcgtccgacc cggcgttcat cgacggccgc cgcggcgaca
     4801 tcatcgtcga tgggaaaatt gtgggggtgt tcggggagat ccacccggcc gccctgaacg
     4861 cgttcgacct tgagcattcg gtcgcagcgt tcgcgctcga ccttaccgcc gtaccgggat
     4921 accccgtccc gccaggtact ccttgacctc gcggatggcg aacacaccgt agtgaaagac
     4981 gcttgccgcg aggcaggcgt cggcttttcc ttctgcaaac ccttcgtaaa agtgttcgag
     5041 cgtcccgacg ccgccgcttg caaccaccgg tatgccaacc gccgtcgcga ccgccgcggt
     5101 gatcgggata tcgaacccgt ccttcgtccc gtcggtctcc atgctggtga ggaggatctc
     5161 gccggccccg cgctcttcgg cctcgcgcgc ccaggcgacg gcatcacgcc ccgttgccct
     5221 gctcccgccg tgggtcacga cctcatacca gcactcccgg ccgtcggcga gggcgatcgg
     5281 cttcttcccc ggttcaaccg cgtaattccg ccggacatcg atggcgacga cgatcgcctg
     5341 cgcgccgaac cgttccgccc cccgggagat caactccggg ttctctacgg cgcttgagtt
     5401 gatgctcacc ttgtccgcac ccgccccgag gatctgctgc atgtcatcga tcgtccgtat
     5461 gccgccgccg acggtgaacg ggagggcgag ctgatccgaa gcccgccgga tcacgtcgat
     5521 gatagtgccc cggtgttctt ttgaggcggc tatatcgagg aagaccaatt catccgcacc
     5581 ctgttcgtcg tagcgccggg ccagagcgac ggggtcgccc gcgtcgcaga ggtcggtgaa
     5641 atgcgtgcct ttgacgaccc gcccgtcctt gaggtccagg caggggatga tccgcttcgt
     5701 cggaaccatg cctactggtg tgaggcgatg ggagatgagg
//
LOCUS       DAOSEE010000131         5620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_5148_length_5620_cov_241.040611, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000131 DAOSEE010000000
VERSION     DAOSEE010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5148_length_5620_cov_241.040611"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(278..1354)
                     /locus_tag="PLI76_09140"
     CDS             complement(278..1354)
                     /locus_tag="PLI76_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI62238.1"
                     /translation="MIECVDVAKRFGSTVVLTGVTARIEAGEIFAVIGPSGSGKSTLL
                     RLIDLLDTPTGGTIRIGGTDIHREPERGLAVRRMMGMVFQKPAAFNTTVYENIAVGLR
                     FRGVGEAAIREKVKEVLRMVGLAGYEERRARTLSGGEMQRVALARAIVIEPDILLMDE
                     PTANLDPVSVALIEDLVLRINRDRGTTIVFSTHDMYQGQRLAHRVGVLMKGVFAQVGS
                     PREVFTLPASREVARFVGVENIVDGVVVSENGSSAVDVGGIRIRARSPFGAGEAVALC
                     IRSEDIRIAPADGGAAAPGRNVLSGTVAAIVPRGPFSRVTVDCGFMITSILSWKKVDD
                     LDIREGSQVEVSFAPESVHVVRRE"
     gene            complement(1359..2060)
                     /locus_tag="PLI76_09145"
     CDS             complement(1359..2060)
                     /locus_tag="PLI76_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI62239.1"
                     /translation="MYEIIEGFIEATKLIATFDPDVMAIAARSIIVSFTATIAATLIS
                     VPLGAAISFGSFRGKKSLINLIQTLYSLPTVIVGLLIFLLISRVGPFGFMRLLFTPTA
                     MIIAQTVLILPIMTGLTIAALSGVDPVIRDTLYSLGATRLQFLVNIMKEARFAILMAV
                     AVGFGRAISEVGAAILVGGNIMASSFASSTRTLTTAISLETSMGNIPTSIALGIILLA
                     IALGVNLAITRIQHR"
     gene            complement(2125..3048)
                     /locus_tag="PLI76_09150"
     CDS             complement(2125..3048)
                     /locus_tag="PLI76_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOI62240.1"
                     /translation="MQRRTLFAVAAVMVALLLICGCTGTTTDPTPAEKQQLRIATTTS
                     LDDTKLLNHLSSIFEKQYNAEVLVISAGTGKALEYGQRGDVDVLMVHDRAREDVFLAD
                     GYGINRRVFAYNFFILIGPEADPAGIKGMEPTKAFATIREKGMTDKNVVFVSRGDASG
                     THAKEQALWKGAGFNYSTDIQGSGDWYLEAGKGMGETLTMANEKEAYTLSDIGTYLAY
                     KGNLDLVTLVDEGDQLLNVYCAMQINPAKYPDINSTIAKDWVNFMISDDVQKEIASFG
                     VDKYGQPLFYAAQKDWEKIGVTEAEVTDPIA"
     assembly_gap    3098..3197
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3210..4016)
                     /locus_tag="PLI76_09155"
     CDS             complement(3210..4016)
                     /locus_tag="PLI76_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridylate kinase"
                     /protein_id="HOI62241.1"
                     /translation="MAERFEMESKLAGESLVRRGLMRKTAGVRQIRIMPDLNVIKIGG
                     HGVIDYGRDVIYPLIEEIGELSRNHKILIATGGGARTRHILDVGIDLGMPTGVLAELA
                     GKISEQNAIMMSLLFSRYNGVRISTGDLLNLPSLISLGMLPVVQGTPPYGLYEHPPEL
                     GSIPPHRTDTGAFLMAEVVGAKNCILGKNVNGLYTENPFINPDAEFIADITADELLKM
                     DLEDMVLEHMAVRLLRDAVHVKEIKIVNAHVPGNITKAVNGERVGTIIRA"
     gene            4110..4343
                     /locus_tag="PLI76_09160"
     CDS             4110..4343
                     /locus_tag="PLI76_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62242.1"
                     /translation="MKIYARERQKVGTGVKQPRFRVVAVVEEKGEAKHLKVESTHFRK
                     IELEQIAKDIGAEVIYLEEMPEEERGEMKAEAA"
     gene            4394..4753
                     /locus_tag="PLI76_09165"
     CDS             4394..4753
                     /locus_tag="PLI76_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62243.1"
                     /translation="MSTYEHDDIFEAAIRILLEEDRCITVSFSPGGVSIRFPTTRKLA
                     EYLDIPHYYVLPRFGIMEHDGLIRRAERVGISTTAAGTVRLLAVMAERYRERAEEVLG
                     REVFSALQARAGAGPER"
     gene            complement(4943..>5620)
                     /locus_tag="PLI76_09170"
     CDS             complement(4943..>5620)
                     /locus_tag="PLI76_09170"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOI62244.1"
                     /translation="NLYLDIMTHDIKNAENVSNLYTDLLIGMLEGETAQYVRRIQSSI
                     QKSAGILRNVTTIRRIHHDASDLGPIDLDAVIRGEIEAFPGIDIEFSGIRCPVWADDL
                     LCEVFSNLIRSAVRFGGSDIRIVIRVEDYDGENVLVSIEDTGPGIPDSVKESFFHRND
                     RGWTEGCGDGLGLFIVSTLIERYGGTIRVEDRVEGRPDLGAAFRFTLREVLHAVDDDD
                     DGDEECG"
BASE COUNT         1057 a         1744 c         1686 g         1033 t
ORIGIN      
        1 accggtagta ggaccgtcgc ctgcacctct cgatcggtct ttgcctccag tgccggcacc
       61 cgatcgcgat cgacccggct cccgtcgccg atgatcagga cgacgatgtc gctctgccca
      121 tcataggtcc tcgctgcggt gttccctgta gaatcgcgac cgcgataccc tccggcgcga
      181 gctcttcgat gccggcccgg acgagaaaaa gggccgtgca ggctcgcttc aactcccgga
      241 cggcaaaaga atcgttatca ggagagagga gccctcctca ctcccgccgg acgacgtgga
      301 ccgactccgg cgcgaacgat acctcgacct ggctcccctc ccggatatcc aggtcatcca
      361 ccttcttcca ggagaggatc gacgtgatca tgaacccgca gtcgaccgtc accctgctga
      421 acggcccgcg ggggacgatg gcggccaccg tgcccgagag aacgttcctc cccggggcag
      481 cggcgccccc atccgccggg gcgatccgga tatcctcaga gcggatgcag agagcgaccg
      541 cttcccccgc cccgaacggc gaccgtgccc ggatgcggat gccgccgaca tcgacagcgg
      601 aagaaccgtt ctcgctgacg acgacgccgt cgacgatgtt ctcgacgccc acgaaccggg
      661 cgacctcccg gctcgccggc agggtgaaga cctcccgcgg cgaccccacc tgcgcaaaca
      721 cgcccttcat cagcacgccg acccggtgag cgagccgctg cccctggtac atatcgtggg
      781 tcgaaaagac gatcgtcgtc ccgcgatcgc ggttgatccg gagcaccaga tcctcgatca
      841 gggcgaccga gaccgggtct aagttcgcgg tcggctcatc catcaggagg atgtcgggct
      901 cgatcactat cgcccgggca agggccaccc gctgcatctc cccgcccgag agcgtccgcg
      961 cccgccgctc ttcgtaaccg gcaaggccga ccatgcggag gacctccttg accttctcac
     1021 ggatagccgc ttcccccacc cccctgaacc ggaggccgac ggcgatgttc tcgtagaccg
     1081 tcgtgttgaa ggcggccggc ttctgaaaga ccatccccat catccggcgg acggcaagac
     1141 ccctctccgg ctccctgtga atatcggtgc cgcctatccg gatcgtccct cccgtgggcg
     1201 tatcgaggag atcgatgagg cgcagcaggg tcgacttccc cgacccggag gggccgatga
     1261 cggcaaagat ctctcccgcc tcgatccgcg ccgtgacgcc ggtgagcacc accgtgctac
     1321 cgaaccgctt tgcgacgtcg acacattcaa tcatcgttct acctgtgctg tatcctggtt
     1381 atggcgaggt tcacgccgag ggcgatggca agcagtatga taccgagcgc tatcgacgtg
     1441 gggatgttgc ccatcgacgt ctcgagcgat atcgcggtcg tcagggtccg ggtcgaactc
     1501 gcaaacgacg acgccatgat gttgccgccg acgaggatcg ccgccccgac ctcggatatc
     1561 gcccggccaa atccgaccgc gacggccatg aggatggcaa accgtgcctc cttcatgata
     1621 ttcacgagaa actggaggcg cgtcgccccg agcgagtaga gggtatccct gatgaccgga
     1681 tcgacccccg agagagccgc tatcgtaagg ccggtcatga tcggcaggat gagcaccgtc
     1741 tgggcgatga tcatcgccgt cggggtgaag agcagccgca tgaacccgaa cggcccgaca
     1801 cgggatatga gcaggaagat gaggaggccg acgatgaccg tcggcaggga gtagagcgtc
     1861 tggatcaggt tgatgaggct ttttttcccc cgaaacgaac cgaagctgat cgcggcgccg
     1921 agcgggacgg agatcagagt ggcggcgatc gtcgcggtga acgagacgat gatgctccgt
     1981 gcggcgatgg ccatcacgtc ggggtcgaac gtggcgatga gctttgtcgc ctctatgaag
     2041 ccctcgatga tctcgtacat actctctcca gtcgtgggtc gtcgaaagca aaaaaagggt
     2101 tggaatcagg ggccatctgc cggatcacgc gatgggatcc gtcacttcgg cctcggtcac
     2161 gccgatcttc tcccagtcct tctgggcggc gtagaagagc ggctggccgt acttgtcgac
     2221 gccgaaagat gcgatctcct tctggacatc atcggatatc atgaagttga cccagtcctt
     2281 cgcaatcgtg ctgttgatat cggggtactt cgcagggttg atctgcatgg cgcagtagac
     2341 gttgaggagc tgatcgccct catccacgag cgtcacgagg tcgaggttgc ccttgtaggc
     2401 aaggtaggtc ccgatatcgg agagcgtata ggcctccttc tcgtttgcca tcgtaagggt
     2461 ctcgcccatg cccttcccgg cctcgaggta ccagtcgccg gagccctgga tatccgtcga
     2521 gtagttgaac ccggctccct tccagagggc ctgctccttt gcgtgcgtcc ccgacgcatc
     2581 gccgcgcgag acgaagacga cgttcttgtc ggtcattccc ttctcccgga tggtggcaaa
     2641 cgccttcgtg ggctccatgc ccttgatgcc cgccgggtcg gcctccggcc cgatgaggat
     2701 gaagaagttg taggcaaata cgcgccggtt gatgccgtag ccgtcggcga ggaagacatc
     2761 ttcgcgcgcg cggtcgtgca ccatcaggac gtccacgtcg ccccgctgcc cgtactcgag
     2821 cgccttgccc gttccggcgg agatgaccag cacttcggcg ttgtactgct tctcgaagat
     2881 cgaactgaga tggttgagga gcttggtgtc gtcaagactc gtcgtcgtag cgatacggag
     2941 ttgctgcttt tctgcaggcg ttgggtcggt cgtcgtcccg gtacagccgc agatgagcag
     3001 gagagcgacc atgacggccg caactgcaaa aagggttctt cgctgcatag ttctcaaaga
     3061 ggtgtcgcaa tacacatatt taaatagggc ggttttgnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnncca gaggggccgt caggcccgga tgatggtgcc gacccgctcc
     3241 ccgttcacgg cttttgttat gttgcccggc acatgggcgt tcactatctt gatctctttc
     3301 acgtggacgg cgtcgcggag gagccgcacc gccatatgct cgagcaccat gtcctccagg
     3361 tccatcttca ggagttcatc ggccgtgata tccgcgatga actcggcatc ggggtttatg
     3421 aacgggttct ccgtatagag cccatttacg ttcttcccga ggatgcagtt ctttgcgccc
     3481 acgacctcgg ccatcaggaa cgcgccggta tccgtccggt gcggcgggat gctcccgagt
     3541 tccggcgggt gctcgtagag gccgtagggc ggggtgccct ggacgaccgg cagcatcccg
     3601 agcgatatga gcgaggggag gtttaagagg tcgccagtgc tgatccggac gccgttgtac
     3661 ctcgagaaga ggagggacat catgatcgcg ttctgctcgc tgatcttgcc cgcgagttcc
     3721 gcaagcaccc cggtcggcat gccgaggtcg atcccgacat cgaggatgtg ccgcgtccgg
     3781 gcgccgccgc cggtggcgat caggatcttg tggttccgcg agagttctcc gatctcttcg
     3841 atcagcgggt agatcacgtc ccgcccataa tcgatgacac cgtgaccgcc gatcttgatc
     3901 acattcaggt cgggcatgat cctgatctgc cggacaccgg cagtctttcg catcaggccc
     3961 cgccggacaa ggctctcgcc cgcaagtttc gactccatct caaaccgttc tgccatgatc
     4021 aaacctcacc atagtgtaca cggggaaagt attaagtatc ttccggatat acctggtgat
     4081 ataccacctg gtattgggat gtggagacca tgaagatcta tgcacgtgag cgtcagaaag
     4141 tcggcaccgg cgtgaaacaa ccccggttcc gcgtcgtcgc cgtcgtcgaa gagaagggcg
     4201 aggccaagca cctgaaggtg gagagcaccc acttcaggaa gatagagctc gaacagatcg
     4261 caaaggacat cggcgccgag gtcatctatc ttgaggagat gcccgaagaa gagcggggcg
     4321 agatgaaggc ggaagcggcg taaccagacc gaccctcttt tttctttccg aaaaccagta
     4381 cttctgcgat cgcatgtcca catacgaaca cgacgacata ttcgaggcgg ccatccggat
     4441 cctccttgaa gaagaccggt gcataaccgt cagcttctcc ccgggcggcg tatccatacg
     4501 gtttcccaca acccggaaac tcgccgaata tctcgatatt ccccactact acgttctccc
     4561 ccggttcggg atcatggagc atgacgggct gatccgcagg gcggagcggg tcggcatctc
     4621 caccacggcg gcgggaacgg tgcggcttct cgcggtcatg gcggagcgct acagggaacg
     4681 cgcagaagag gtcctcggca gggaggtctt ctcggcgctg caggcccgcg ccggcgcggg
     4741 gccggagagg tgaaggactc tatcacgacg catggcggca cgcaccaaaa gatcccctgc
     4801 gcggtcgacg atgcggcgcc ggcaccctcc cccttcatgt aaggcccggc cgttgcggga
     4861 gatcccccgg gtgcagaagg atgtatcgca gccaagatac gctgcttagc gcacccggag
     4921 gagggaaggg gcgtagaggg cctcacccgc actcttcatc gccgtcatca tcatcgtcga
     4981 cggcatgcag gacctcgcgg agggtaaacc ggaacgccgc gccgaggtcc ggccgccctt
     5041 ccacccggtc ctccacccgt atcgtaccgc cgtaacgctc gatgagggtg ctgacgatga
     5101 agagaccgag cccgtcgccg cacccctcgg tccacccccg atcgtttcgg tgaaagaacg
     5161 actccttcac ggagtcaggg atacccggac cggtatcctc gatggagacg aggacgttct
     5221 cgccgtcgta atcctccacc cggatgacga tccggatgtc cgaacccccg aacctgacgg
     5281 cactccggat gaggtttgag aagacctcgc agaggaggtc gtccgcccag accgggcagc
     5341 gaatgcccga aaactcgatg tcgataccgg gaaaggcctc gatctcccct ctgatgaccg
     5401 cgtcgaggtc gatcggcccg agatcggatg catcgtggtg gatccggcgg atcgtggtga
     5461 cgttcctgag gataccggcg ctcttctgga tgctgctctg gatcctcctc acgtactgcg
     5521 cggtctcgcc ctccagcatc ccgatgagga gatccgtgta aaggttcgat acgttctcgg
     5581 cgttcttgat atcgtgcgtc atgatgtcga gatacaggtt
//
LOCUS       DAOSEE010000132         5609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_5166_length_5609_cov_255.907274, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000132 DAOSEE010000000
VERSION     DAOSEE010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5609
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5166_length_5609_cov_255.907274"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(264..1811)
                     /locus_tag="PLI76_09175"
     CDS             complement(264..1811)
                     /locus_tag="PLI76_09175"
                     /inference="COORDINATES: protein motif:HMM:NF040080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6398 domain-containing protein"
                     /protein_id="HOI62245.1"
                     /translation="MACLQASGWLTGKKPSLLIMIQGRYNKNYWLVVLARHDARLREL
                     DRLIRDVWVECCDHLSSFKIGGVAYESDSTFSTGTMSVPLARLVSPGSTFTYDYDFGS
                     TTSLDLKVIGETPIALPGGPLCLIARNDPPAIPCDLCGGKAAVILDDPDDDIPHYYCR
                     ACLVSTEHDPDYVETIANSPRNGVCGYTEDIAAALSWYPPGRSTGEVVSEEPDEVLPD
                     DDAEVNAAIVAVIQDIGPDIDAFVQEEGAAHGDRGACMAGDTILAFCTFMHLLSGTKI
                     DAWDVPSVQRCLIEHLAQNLLTPDDWLKNAVPILCRFLSRMEASGRITNASELIAALK
                     QAEPAFQEAATSPEKGRAIFEYILMKAEEAGISITDIDASFAFAVKELARMAGIDPDD
                     EEVRKRLSGLLDSGITEAFSSKLRATMIFGRCDEFCRRFKDDTILERCRRIFTALADH
                     PAEPLLRGDGALWSAAIIYAACQDEDLIRPGKGAPPLGQEIGFFFGFERSSIRNKVRA
                     MRALLPE"
     gene            2142..2459
                     /locus_tag="PLI76_09180"
     CDS             2142..2459
                     /locus_tag="PLI76_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62246.1"
                     /translation="MEVLRTRIVIDWVRKDGLIFASDYSDCDHSAEGFRKVMFRIKGG
                     VPLKAGAAYSLEYETEDILKPFVQKSRSGKTTSYYPHRFYHKIRNVTEVSANPDADLL
                     FDL"
     gene            complement(3086..3445)
                     /locus_tag="PLI76_09185"
     CDS             complement(3086..3445)
                     /locus_tag="PLI76_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOI62247.1"
                     /translation="MQDECTVNQTVKYLGKKWTLLIILELYKGDGYTRRFTEMKERLP
                     GITAKVLSARLKELEEEGLVEKKIDTKTIPVRSEYTLTESGLGLVEVIKDIKRWALRW
                     KIENLACGAQDCRDCVL"
     gene            3531..5147
                     /gene="hcp"
                     /locus_tag="PLI76_09190"
                     /gene_synonym="priS"
     CDS             3531..5147
                     /gene="hcp"
                     /locus_tag="PLI76_09190"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOI62248.1"
                     /translation="MFCNQCEETAKGFGCTVRGVCGKDEEAAGLQDVLIYTLKGLSTR
                     NLAAMKAGGGNPDAGKFVAGCLFATLTNVNFDPARLRDMINEAARIRDALPPAGSTEP
                     AACTWAPASPAAIAAKAQEIVAAREAVDPDLQSLRDLLVYGLKGLGAYYHHAAILGYE
                     DEEITVFMQEALAATLQDLSVGDMVGHVLACGEVGVKVLALLDTANTTAYGTPAITTV
                     RTTAGARPGILVTGHDLKDLADLLEQARGAGVDVYTHGEMLPAHAYPDLRKYDHLVGN
                     YGGSWPFQREEFERFNGPVLVTTNCLVPPKDSYRDRVYTTGLVGYAGLKHIDASPDGK
                     KDFSTLIEHAKRCKAPEDISRGDLVTGCAHAPVLALADTVIDAVKTGAVKRFLVMAGC
                     DGRYGGRDYYTRFAKALPADTVILTAGCAKYRYNSLGLGDIGGIPRVLDAGQCNDCYS
                     LVVIAKALAEAFGVGINDLPVSYNIAWYEQKAVLVLLSLLSLGVKDITLGPRLPAFVS
                     PGILKVLVENFGIRGIATVEEDLARMVPGN"
BASE COUNT         1063 a         1714 c         1720 g         1112 t
ORIGIN      
        1 cggaacagca ggcgttcgcg agccgggggc tcgacttcgt gacgaaggag tacctgccga
       61 agaggcttgc ggagatgggg gttatctgag gcggaccccg gcggttctcc ggtaccctgt
      121 aacttccatg gaggttttgc ggtggccccc tccgggacga cgcgctctct cgcatacggg
      181 gagcgattga gcgtcatgct gccgcaggca gagggccggc ggccggcaac cttcgttgcc
      241 gggagttctg cttccggccg gtttcactcg gggaggagcg ctctcatcgc cctcaccttg
      301 ttccggatcg atgaccgctc aaagccgaag aagaaaccga tctcctgccc gaggggagga
      361 gcgccctttc ccggccggat caggtcttca tcctggcagg ccgcatagat gatcgccgcg
      421 ctccagagtg ccccgtctcc ccgcaggagc ggttcggccg gatggtcagc gagagccgtg
      481 aagatcctcc ggcaacgctc aaggatggtg tcatccttaa accgcctgca gaactcatcg
      541 cacctcccga atatcatggt ggcgcgcagt ttgctggaga atgcctctgt tattccgcta
      601 tcgagcaggc cggagagcct ctttcgaacc tcttcgtcgt cagggtcgat cccggccatc
      661 cgggcaagct ccttcactgc gaaggcgaag gatgcatcta tgtcggttat gctgatgcct
      721 gcctcctctg ctttcatcag tatgtactcg aagatggccc gacccttctc cgggctcgtg
      781 gctgcctcct ggaaggcggg ttcagcctgc ttgagggcgg caatgagttc ggaggcgttg
      841 gtgatgcggc cggatgcctc catgcgcgag aggaaccggc agaggatggg gacggcgttc
      901 ttcagccagt catccggagt gagcagattt tgcgccagat gttcgatcag gcacctctgc
      961 acggacggaa catcccatgc gtcgatcttt gttccagaga ggagatgcat gaacgtacag
     1021 aaggccaaga tagtgtcccc ggccatacag gcgcccctat ccccgtgtgc cgccccctct
     1081 tcctggacaa acgcatcgat atcgggtccg atatcctgga tgacggcaac aatcgcggcg
     1141 ttcacctccg catcgtcgtc gggcaagacc tcgtccggtt cttccgaaac gacctcgccc
     1201 gtgctccgcc ccggcggata ccaggagagt gcggcggcaa tatcctcggt atagccgcag
     1261 acgccgtttc ttggggagtt ggcgatggtc tccacgtagt cggggtcgtg ttcggtcgat
     1321 acgaggcatg cgcggcagta atagtgcggg atgtcgtcgt caggatcatc caggataaca
     1381 gcggccttgc cgccgcagag atcgcagggg atagccggcg gatcgttccg tgcgatcagg
     1441 cagagcgggc cgcccggcag ggcaatgggc gtctccccga tcaccttcag gtccagcgat
     1501 gtggtggagc caaagtcgta gtcataggtg aacgtgctgc ccggggagac gagacgcgcg
     1561 agggggacgc tcatggtgcc tgtggagaat gtactgtccg attcgtaggc gactcccccg
     1621 atcttgaagg aactgagatg gtcgcagcac tccacccata catcgcggat caaccggtcg
     1681 agttctctaa gccgggcgtc gtgtcgcgcg agaacgacca gccagtagtt tttgttgtac
     1741 cgcccctgta tcatgatgag gagggagggt ttcttcccgg tgagccagcc cgacgcctgg
     1801 aggcaggcca tgccgtgctt gagagccttc cgttatgtca ggggagccct gcagagcagg
     1861 caggtaccgg gtgagacctt tgatgccatt caccagaagg tatgatagtc agggataatg
     1921 aatgatcgtg ggtctgcccg cctccccgga ggatacgacc cgggcaccct ccgcatgccc
     1981 ggactaccgc gtttagtggg tttgatgaac gttaatgact gttttgggaa tttcggcggc
     2041 tatctcttat gggcgaggca ctttcgctac gatcactttc acgccgcacg gcgcgcctta
     2101 aagcctgatg aggacaacag caattcaata taggattggt gatggaagtg ctgcgcacca
     2161 ggattgtgat cgattgggtc cggaaggatg ggcttatctt cgcctcggat tactcggatt
     2221 gcgaccactc agcggagggt ttccggaagg tcatgttccg aataaaagga ggagttcccc
     2281 tcaaggccgg agcggcgtac tcgcttgagt acgagacgga ggatatcctc aaacccttcg
     2341 tgcagaagag ccggagcggg aagacgactt cctactatcc ccacaggttc tatcacaaga
     2401 tcagaaacgt cacagaagta tcggccaacc cggatgcgga tctcctgttc gacctctgat
     2461 ccctccagga atcgcatcct ccgggcatgg tgtatggagc ggtacagcat cccgaatccg
     2521 gaggagcggt cttctgaacg gccggcaacc ctgtggcggc ccggcagttc atgctaccgg
     2581 agcgcgcaca caggttccgg agaggcgtca cagtttttcc agccattcgg tgatcaggag
     2641 aatgggtatt accggaactg agagaacact cttcacgccg acggcgactc atcgggtgcc
     2701 ctgagtagtt actggtaatc aatagaagtg tcgagtatac tcgatgataa tcaataaaag
     2761 cgtcgattat gatctacgat tccctatgcc ccggagagag cgtccgagga actccccctg
     2821 cgcatgccgg tgatgggagc ggtgcccctt cgtctggctc acgaatcccc ccgcacggct
     2881 aaccttcatg ccggaggcgg tcaaacacga tacttgcggc gggggcgccg gcacggttcc
     2941 ggccgctgcc gccggtggca aagcggtgat gcggggtatc cccgccagcc ggggcagccc
     3001 gcctgtgaga gcaagcgtgc atgctatccg gtggacccgc cggggaatga ttggtgcaaa
     3061 gtcccgtccg cgagggcagg gtcgttcaca aaacacagtc gcggcagtcc tgcgccccgc
     3121 acgcgaggtt ctcgatcttc caccggagcg cccaccgctt gatatccttg atcacctcga
     3181 cgagcccgag cccgctttcg gtgagggtgt actcgctcct gacggggatg gtcttggtat
     3241 cgatcttctt ctcgacgagg ccctcctcct ccagctcctt caggcgggcc gagaggacct
     3301 ttgccgtgat ccccgggagc cgctccttca tctcggtgaa ccgccgggtg taaccgtccc
     3361 ccttgtagag ctcgaggatg atcaggagcg tccatttctt cccgagatac ttgacggtct
     3421 gattgaccgt gcactcgtcc tgcatggtat aattagagaa accttctcct ccttatactt
     3481 tagtatctaa ttgatacctt atatcaaaaa gatagtatgg agtgagatga atgttctgca
     3541 accagtgtga agagacggca aagggtttcg gctgcaccgt acgcggcgtc tgcggcaagg
     3601 atgaggaggc tgccggactc caggatgtcc tgatctacac cctcaagggg ctctcgacga
     3661 ggaaccttgc ggcgatgaag gcgggcggcg ggaacccgga tgccgggaag ttcgttgcgg
     3721 gatgcctctt tgcgaccctg accaacgtga acttcgatcc tgcccgcctc cgggatatga
     3781 tcaatgaggc ggcccgcatc cgcgacgccc tcccgcccgc cgggagcacg gagcccgcgg
     3841 cctgcacctg ggcgccggca agcccggcgg cgatcgcggc aaaggcgcag gagatcgtcg
     3901 ccgcgcggga ggccgtggac cccgacctgc agtcgcttcg tgacctcctg gtctacgggc
     3961 tcaaagggct cggcgcctac taccaccacg ccgccatcct cggctacgag gacgaggaga
     4021 tcacggtttt catgcaggag gccctcgccg ccacgctgca ggatctctcc gtcggggata
     4081 tggtcggcca tgttctcgcg tgcggcgagg tcggggtgaa ggtccttgcc ctcctcgata
     4141 ccgcgaacac cactgcctac gggacgccgg cgatcacgac cgtccggacc acggcgggcg
     4201 cgcggccggg tatccttgtc accggccacg acctcaagga cctcgccgac ctccttgaac
     4261 aggcgcgcgg ggcgggtgtg gatgtttaca ctcacggcga gatgctcccg gcgcacgcct
     4321 atcccgatct tcggaagtac gaccacctgg tcgggaacta cggcggctcc tggcccttcc
     4381 agcgggagga gttcgagcgg ttcaacggcc cggtcctcgt gactacgaac tgcctggttc
     4441 ccccgaagga ctcttaccgc gaccgggtct acaccaccgg gctcgtcggg tatgcgggac
     4501 tgaagcacat cgatgcgtca ccggacggga agaaggactt ctcgaccctg atcgaacatg
     4561 cgaagcgctg taaggctccg gaggacatct cccgcggcga cctcgtcacg gggtgcgccc
     4621 atgccccggt tctcgccctc gcggatacgg tcatcgatgc ggtgaagacc ggcgccgtca
     4681 agcggttcct cgtgatggcg ggctgcgacg gccgatacgg cggacgggac tactacaccc
     4741 ggttcgcgaa ggctcttcct gcagatacgg tcatcctcac cgccgggtgc gcgaagtacc
     4801 gctacaacag cctcggtctc ggggatatcg gcggcatccc gcgggtcctc gacgccggcc
     4861 agtgcaacga ctgctactcg ctcgtggtca tcgcaaaagc cctcgccgaa gcgttcgggg
     4921 tcggtatcaa cgacctcccg gtctcttaca acatcgcctg gtacgagcag aaggcggtgc
     4981 tcgtcctctt aagtctcctc tcgctcggcg tcaaggatat caccctcggc ccccgcctcc
     5041 cggccttcgt ctcgcccggg atcctcaagg tgctcgtcga gaacttcggg atccggggga
     5101 tcgcgacggt cgaggaggac ctcgcccgga tggtgccggg gaactgaaca cctctttttc
     5161 ggggcgggca ggttttgcag gtttaccccg cacccggggc tccggttcgg tatctacgtt
     5221 gcacacctga caccatcacc cctgataacg gcacccggcc ctctaaaaga gatcccgaac
     5281 ttcttccaga gcgtagatga gaaggaaaag ttcagtcagg ctcatcagcg gcttaacgtg
     5341 attgagttat aactttcccg ggatgtgcca gtgcgagaaa cgccctcggg gtatggacaa
     5401 accgccttta tccggcttca gatcgcttcg cagcgttcag tctccgcaca acatatccag
     5461 tgacagttcc tgtgtctcat aactcttctt tcagtccctt caaccactcc cggagcacag
     5521 gcatatcgga ctgcaccgtc tcccagacga tctcaaggct gacgccaaaa tagtgatgga
     5581 tgagtttatc ccgcattccg gcgatgaac
//
LOCUS       DAOSEE010000133         5570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_5212_length_5570_cov_258.584587, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000133 DAOSEE010000000
VERSION     DAOSEE010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5212_length_5570_cov_258.584587"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PLI76_09195"
     CDS             <1..397
                     /locus_tag="PLI76_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62249.1"
                     /translation="VTMQLKQSVLLALGILLVLAGVVLQYTGIVVESTATVALVSAGV
                     VMVIVAVITGFSRQDEVISDERTKRIGAYGTTYSWVLTLVLILAFLWLDFLGLVSFNL
                     QGTLLVLLFTMLISARIFQWYLFRQGDVA"
     gene            447..653
                     /locus_tag="PLI76_09200"
     CDS             447..653
                     /locus_tag="PLI76_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOI62250.1"
                     /translation="MKTRIRELRAKTGLTQEELAREVGVRRETIVFLEQGKYNPSLKL
                     AYKVARVLGASIEEVFMFEEEDLA"
     gene            complement(1074..1619)
                     /locus_tag="PLI76_09205"
     CDS             complement(1074..1619)
                     /locus_tag="PLI76_09205"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HOI62251.1"
                     /translation="MIGNLLITGRPGSGKTTLMRRLAERLRDCSPVGFYTVEVREGGV
                     RVGFDLVSLAGDRRPFARTGYPGRCRVGRYGVDIEGFEDFLSSIPFFTPGTRLAFIDE
                     IGKMECYSGVFRGIVREALDAPAPCVATIAQRGMPGLDRIRGRADVRVVEVTPSNRDG
                     LLPELEAEARRLVGDAGQGWK"
     gene            complement(1669..2721)
                     /locus_tag="PLI76_09210"
     CDS             complement(1669..2721)
                     /locus_tag="PLI76_09210"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOI62252.1"
                     /translation="MPGGSNPPGRMGYPFDRAGRGWRAGGRGLFRESIAPGCDQARHI
                     RDDLIAEYRGLTVEDAFSGREVACPTGSCYVLESRSPMTEGLDSRDPERAAAAILADL
                     TLVPGIGGATRQRLQRQGYRTIADLVRHPLYCRDAARILDGVLGRDTCGLMEWIGRRH
                     RQSDPLLLEVSRFHAPETFVFLDIETLGLSSRPIVLIGLARVEGDAIAIRQYLLRSLN
                     EEAAALATALPDLEAEGAALVTFNGRAFDLPFIRERLVRYGIPADLGAPHFDALLFAR
                     RRWKGSIPACRLNVLETEVLGVERQDDLPGWMVPEFYDIYRRTGNPGPLIPVVEHNRQ
                     DLVSLARLFALLRGDE"
     gene            complement(2765..3268)
                     /locus_tag="PLI76_09215"
     CDS             complement(2765..3268)
                     /locus_tag="PLI76_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62253.1"
                     /translation="MDVFERIPMDVRWKLAAREISALPLAYRRAIRDGADDYLYNEVE
                     RAVWQEQGREVGVIARAFGMPTGNARGVAEAFAAATRVFFGPEYRYEIQESGSDEALL
                     TVRGCAMVARADERGEDPLTVCNACHAYANAAIPALNPDYAPKYRSSICAGDRACSIA
                     VRPAGRE"
     gene            3389..3553
                     /locus_tag="PLI76_09220"
     CDS             3389..3553
                     /locus_tag="PLI76_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62254.1"
                     /translation="MKVRAVPIDIGGARTSGTKGIPAGTDRRRERAVRRSVPVPDLVV
                     GLLADLLDHP"
     gene            3558..4661
                     /locus_tag="PLI76_09225"
     CDS             3558..4661
                     /locus_tag="PLI76_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62255.1"
                     /translation="MERKLLATLILVAMALGAGCADIFPGPPVALPAIVPVEGASLDP
                     LPAHTFPFEGGEETIRIDPDPAVYAGAKGADRRLHLARDLSREEWIPLYYRAFESEPH
                     LEPFYADLLAALRDIRDREGLDDDRYLELIAVFVQSIPYRTDASIVEPKFPIETYVDG
                     EGDCDDRSLLLAGLLAREGYGVALFYFEDEAHMAVGVQSTGCHYRNTSLAYVETTNVS
                     YVGIPPLALSSGVVLASDPLVIPVGDGPRLYTGCDQIETIERALSSSLARAGALAPEL
                     AARTRDIEAERESLEAFGTRLKALSRDGEFLEYNRLVPEYNRKTREYNDAVQAYNALL
                     AESRAAVDLHNHLVTHACDRPGSYLRARAYLAG"
     gene            complement(4725..4880)
                     /locus_tag="PLI76_09230"
     CDS             complement(4725..4880)
                     /locus_tag="PLI76_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62256.1"
                     /translation="MEETAFATFACLAERAARYPTIVRRSQMCKAAYASIAAPADRTR
                     LKAEGRA"
     gene            5118..>5570
                     /locus_tag="PLI76_09235"
     CDS             5118..>5570
                     /locus_tag="PLI76_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOI62257.1"
                     /translation="MLDISDLHVNVDDTEVLHDINLHIGQGETHVLMGPNGSGKSTLL
                     KAVMGFGGYTITAGSIVFRGTDITHMPIHERAHLGIGMMFQHPPAISGLKLGKLLTAT
                     SHMGEGAIEALAQSVNMERFLTRDINVGFSGGEIKRSEVLQLKVQQPDF"
BASE COUNT         1037 a         1799 c         1749 g          985 t
ORIGIN      
        1 ggtgacaatg cagttgaaac agtccgtact tctggccctc ggcatcctgc tcgttcttgc
       61 aggtgtcgtc cttcagtata ccggcatcgt cgtcgaaagc accgctacgg tcgcgctcgt
      121 ctcggcgggt gtcgtcatgg taatcgtggc ggttatcacc ggctttagca ggcaggacga
      181 agtcatctcc gatgaaagaa cgaagaggat tggcgcttac ggcaccacct actcctgggt
      241 gctcacgctg gtactgatac tggccttctt atggttggac ttcctcggtc tggtgtcgtt
      301 caatctccag ggaacgcttc tggttctgct gtttaccatg ctcatctccg cacggatctt
      361 ccagtggtac ctcttccggc agggagacgt ggcgtgaggc aggcagatgc cgccgaaagg
      421 agtctctctc ggaaggggga cgtcgggtga agacccggat ccgggaactc cgtgcgaaga
      481 cggggctcac ccaggaggaa ctggcgcgag aggtcggcgt gcggcgggag acgatcgtct
      541 tcctcgagca ggggaagtac aacccttcct tgaagctcgc ctacaaggtg gcccgggtgc
      601 tcggcgcctc catcgaggag gtcttcatgt tcgaggaaga ggacctggcg tgaccgcgcc
      661 ggagccgtag gacggcagag aaaccccgcc cgtgacgttg ccgccagagc cccggcggtg
      721 acggaggtcg tttggcgggc gcaaccggct tttccggagg gccgaacggc aggtgagcag
      781 gaaagaaggc agagcaccgg gcagacgccc ccgcgacgat atgcgggaca gaagagaagt
      841 cggaaaaata tattgggggc aattgttaag gttgtaatat aaggttaaaa acattccggt
      901 ttttaattct ggcagaatct cggtagattc ccataaaaag ccttatatca ttatatttcc
      961 ggaaagtgcg tttttattgt gcgataaata tattatctcc cggcaatttg aacattcggg
     1021 gtgggcgcag cactatggaa ggttacttcg gtttttcgcc ggatctgcgt cgatcatttc
     1081 cacccctgtc ccgcatcccc caccaggcgc ctcgcctctg cctcgagctc cggcaggagg
     1141 ccgtcacggt ttgatggggt cacctcgacg acccgaacgt ccgcccggcc cctgatccgg
     1201 tcgagtcccg gcatgccccg ctgtgcgatc gtcgccacgc agggggcggg agcatcgagc
     1261 gcctcccgta caatcccgcg aaagaccccc gaataacact ccatcttccc tatctcatcg
     1321 atgaacgcca ggcgggtgcc cggtgtgaag aacgggatcg acgagagaaa atcctcgaac
     1381 ccctcgatat cgaccccgta ccggcccaca cggcagcgac ccgggtatcc ggtgcgggcg
     1441 aagggtcgcc gatcgccggc aaggctcacg aggtcgaacc ccacccgcac tcccccttcc
     1501 ctgacctcga cggtgtagaa cccgaccggc gagcagtcgc gaagacgctc tgccagacgg
     1561 cgcatgagcg tggttttccc ggaaccgggc cggccggtga tgagcaggtt cccgatcacc
     1621 gttccgcggc ctccccggtg cggaccctcc ggcggcagac cggcaccatc actcgtctcc
     1681 cctgaggagc gcaaagagcc gggcgagcga gacgaggtcc tgccggttat gctccaccac
     1741 cggtatgagc ggaccggggt tcccggtccg ccggtagata tcgtagaact ccggcaccat
     1801 ccagcccggg aggtcgtcct gccgctcgac cccgagaacc tccgtctcaa gcacgttgag
     1861 ccggcaggcg ggtatcgagc ccttccagcg ccgccgggca aagaggagcg catcgaagtg
     1921 aggcgctcca aggtccgccg gaataccgta gcgtacaagc cgttcccgga tgaaggggag
     1981 gtcgaacgcc cggccgttga aggtgacgag ggccgcaccc tccgcttcca ggtcggggag
     2041 ggcggtggcg agcgccgccg cctcctcgtt gagggaccgg agcaggtact ggcggatggc
     2101 gattgcgtca ccctccaccc gggcaagccc gatgaggacg atcggccggg aggagagccc
     2161 cagcgtctcg atatcgagga agacgaacgt ctcgggagcg tggaaccggc tgacctcgag
     2221 gaggagcggg tcgctctgcc ggtgccgccg cccgatccac tccataagac cgcaggtatc
     2281 cctccccaga accccatcga ggatccgcgc ggcatcccgg cagtagaggg gatgccggac
     2341 gagatcggcg atcgtccggt aaccctgccg ctgcagcctc tgccgggtcg cgccaccgat
     2401 accggggacc agggtgagat ccgcaaggat ggccgctgcc gcccgttccg ggtcacggct
     2461 atcgagccct tccgtcatgg ggcttctgga ttcaaggacg tagcaactcc cggtggggca
     2521 tgcaacctcc cggccagaga aggcatcctc gacagtcagg ccgcggtatt cggcgatgag
     2581 gtcgtcccgg atgtgccggg cctgatcgca gccgggcgcg atgctctccc gaaaaagtcc
     2641 gcgcccaccg gctcgccacc ctctccccgc acgatcgaac ggatacccca tcctgccggg
     2701 agggttagat cctcccggca ttcaaaggtg atccctgcgg ggtgaatgcg agcggcaggg
     2761 cacgtcactc ccgacctgcc ggcctcaccg cgatcgagca cgcgcggtcg ccggcgcaga
     2821 tgctgctccg gtacttcggc gcataatccg ggttcagggc cggtatcgcg gcgtttgcgt
     2881 aggcgtggca ggcgttgcag acggtaagcg gatcctcccc tcgctcatct gctcttgcca
     2941 ccatcgcgca cccccggacg gtcagcaacg cctcgtccga tcctgattcc tggatctcat
     3001 accgatactc gggcccgaag aagacccggg ttgccgccgc aaacgcctct gcaacccccc
     3061 gtgcgttccc ggtcggcata ccgaacgcgc gggcgatgac gcccacctct ctgccctgct
     3121 cctgccacac cgcccgttcg acctcgttgt agagatagtc gtcggcgccg tccctgatgg
     3181 cccgccggta ggcaagcggc agcgcggaga tctcccgggc cgccagcttc cacctcacat
     3241 ccattggaat tcgttcgaat acatccatac gctctccctc catctcgtgt cggcctccat
     3301 gccggaggtg gctatacccg caacccacat gaacgttcgc ccgggaggca ggcgcgcgca
     3361 taaatacccc ggcgagcaac aggagagcat gaaggtcaga gcagtcccga tcgatatcgg
     3421 tggcgcccgg acctccggga cgaagggaat cccggccggg accgacaggc gccgggagag
     3481 ggcggttcgc cggtcggtcc ccgtaccgga tctcgttgtc ggcctgctcg ccgacctcct
     3541 cgatcacccc tgataccatg gaaaggaagc tcctcgccac gctcattctg gtcgccatgg
     3601 ccctcggggc cgggtgcgcg gacatcttcc cgggcccgcc ggttgccctg ccggcgatcg
     3661 tgccggtcga gggggcgagc cttgaccctc tcccggccca caccttcccc tttgagggcg
     3721 gagaggagac gatccggatc gatcccgatc cggcggtcta tgccggggca aaaggggcgg
     3781 accgaaggct tcatctcgca agagacctct cccgggaaga gtggatcccc ctctactacc
     3841 gggcgtttga gagcgaaccc cacctggaac ctttctatgc ggacctgctt gcggccctcc
     3901 gcgatatccg ggaccgcgag gggcttgacg acgaccgata cctcgaactg atcgccgtct
     3961 tcgtgcaatc cattccttac cggaccgacg cctcgatcgt ggagccgaag ttccccatcg
     4021 agacctatgt cgacggggaa ggcgactgtg acgacaggag cctcctcctc gccgggctcc
     4081 tcgcacggga gggttacggg gtcgcgctct tctacttcga ggacgaggcg cacatggccg
     4141 tcggcgtgca gagcaccggg tgccactacc gtaacacctc ccttgcctac gtcgagacca
     4201 cgaacgtgag ttatgtgggc atcccgcctc tggccctctc gagcggggtg gtgctcgcct
     4261 cggatcccct ggttatcccg gtcggtgacg ggccgcgcct ctacacgggg tgcgatcaga
     4321 tcgagacgat cgaacgcgcc ctctcctcct cccttgcccg ggccggggca cttgcgcccg
     4381 agctcgcggc gcgcacgcgg gatatcgaag cggagcggga gtctctcgag gccttcggca
     4441 cgcggttgaa ggctctctcc cgggacgggg agtttttgga gtacaaccgt ctcgtcccgg
     4501 aatacaaccg gaaaacccgg gaatacaacg acgcggtgca ggcctacaac gccctgctcg
     4561 cggagtcgag ggcagccgta gaccttcaca accatctcgt cacccatgcc tgcgaccggc
     4621 ccgggtcgta cctcagggcc cgggcgtatc tcgcgggatg atgcacgacg atcggttgag
     4681 cgggggccgg gcgagagcgg tcttgcaagt accccggaga ggtctcaagc gcgcccctct
     4741 gctttgaggc gcgtacgatc cgcgggggca gcgatgctgg catacgctgc tttacacatc
     4801 tggctccgcc ggacaatggt cgggtatcgg gctgcccgct cggcgaggca ggcaaacgtt
     4861 gcaaacgcag tctcttccat acaaagaaca agggcgaggc ctctatttaa ccaggaggcg
     4921 tgcggggtga aagtgaaagt caggggcaac ggtcgtctct cctgtctcgt ttgcgcgtta
     4981 agccgcggca ccggcaggcg ttcatgaaaa aatccctgta tcgagcgggt ttgcccctac
     5041 aggggcagaa acggcaactg ttccgttggc aagtgttaat gtatcccctt gaccaataca
     5101 tgaaacggag tgccgttatg ctggatatca gtgatctgca cgtgaacgtg gatgacaccg
     5161 aagtgctcca cgatatcaac ctccacatcg ggcaggggga gactcatgtt ctgatgggcc
     5221 cgaacggttc ggggaagagc acgctgctca aagccgtcat ggggttcggc ggctacacga
     5281 tcacggccgg atccatcgta ttcaggggaa cagatatcac ccacatgccg atccacgaac
     5341 gggctcatct cgggatcggc atgatgtttc agcacccgcc tgctatatcc gggctgaaac
     5401 tcgggaaact gctcaccgcc acgtctcaca tgggagaggg cgcgatagag gcgctcgcgc
     5461 agtcggtcaa catggagcgt ttccttaccc gggacatcaa cgtcgggttc tccggcggcg
     5521 agataaaacg gagcgaggtt ctgcaactga aggtccagca gcccgacttc
//
LOCUS       DAOSEE010000134         5567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_5217_length_5567_cov_254.173077, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000134 DAOSEE010000000
VERSION     DAOSEE010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5567
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5217_length_5567_cov_254.173077"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..694
                     /locus_tag="PLI76_09240"
     CDS             146..694
                     /locus_tag="PLI76_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOI62258.1"
                     /translation="MERDEALALLRRYVVSPSHIAHSYATAGIMKRVAEHLGEDAGTW
                     EIIGLLHDIDYDLVAGDMDRHGIEGHRILIENGVPEEIADIVRRHNHTHFSDYVRPVE
                     IALQAADSASGLIIACALVKGGAVSEVAPRTVKKKFKEKSFAAGCERERIRMIEPLMD
                     METFFRLAIEGIMDIRTDIGLT"
     gene            734..1285
                     /locus_tag="PLI76_09245"
     CDS             734..1285
                     /locus_tag="PLI76_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate synthase family protein"
                     /protein_id="HOI62259.1"
                     /translation="MTGVDRARVIERMNEAMMLLTERMDARLIPETGMNIAYALPDAR
                     SKDDVAAVPGGIVGHADRVHPAGDIAFGADAYVARIVITAMRFDPAIRSAATIRFSEA
                     VLIEMENLLFEICSFDPEKEPPGVQTMDWGVASCCREGVPDVIYDRGAAGKEPTIRLL
                     GEDPVTVARNLLKLSNRITYAQL"
     gene            1298..1486
                     /locus_tag="PLI76_09250"
     CDS             1298..1486
                     /locus_tag="PLI76_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17e"
                     /protein_id="HOI62260.1"
                     /translation="MGIKPSYIKNLGEELIVKHRERFSGDFEENKHAVSEVATIGSKS
                     VRNRVAGYISRKINTRKR"
     gene            1497..2372
                     /gene="dapA"
                     /locus_tag="PLI76_09255"
     CDS             1497..2372
                     /gene="dapA"
                     /locus_tag="PLI76_09255"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOI62261.1"
                     /translation="MFEGILPAIITPFHRDSGMSLDIEGLRSNIESLLQRGIHGIVPC
                     GSTGESATLTFEEHEQVISKTVEVVNGRIPVLAGTGSNNTEEAIRLTRAAKDAGADGA
                     LVISPYYNKPNRSGLVKHFTKLADIGLPIVLYNVPGRTGQNLLPDLVIELARHPNIVG
                     VKEASGDIVQISRIIEGTRDEEFVVLSGDDAMTLPVLALGGAGVISVTANVDPGRMVQ
                     MYEAFRAGDLARARDLHFELSPLMRAMFIDTNPIPVKKAVGLSGMAAGPVRLPLDDLD
                     GQKTEQLRKVLASYD"
     gene            2365..3123
                     /gene="dapB"
                     /locus_tag="PLI76_09260"
     CDS             2365..3123
                     /gene="dapB"
                     /locus_tag="PLI76_09260"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOI62262.1"
                     /translation="MIKVAVSGALGRMGANIGRLVNEAPDLTLVGGIDVREGTLFETE
                     VVPAAKIDEFLKEKRPDVLVDFTVAAAAVENVRAAARNGVALVVGTTGFSPEQREVIR
                     GAVEGNVPAVISSNFSVGVNIFWKLVREAARELGDYDIEVTEAHHRHKKDAPSGTAKT
                     ILDILDEEIGNREKAYGRVGMTERKNEIGVHVIRGGDIVGDHAVLFAGNFECIEISHR
                     AYDRAVFAQGAVRAVRWVVGKEPRMYTMQDVLGI"
     gene            3205..3378
                     /locus_tag="PLI76_09265"
     CDS             3205..3378
                     /locus_tag="PLI76_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOI62263.1"
                     /translation="MPAVVDIEKCTACETCIDVCPASAITMEDGKAKVDPDLCVDCET
                     CVDECPSEAISME"
     gene            3425..4441
                     /gene="asd"
                     /locus_tag="PLI76_09270"
     CDS             3425..4441
                     /gene="asd"
                     /locus_tag="PLI76_09270"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOI62264.1"
                     /translation="MIHVGVLGATGAVGQRFVQLLADHPWFHLQTLTASERSAGKPYG
                     KVVNWRLDAPYPDNVSDIVVTPTTVESLKDCDLVFSALPADVAGPFETELANAGIAVC
                     SNARSHRMEPDVPLVIPEVNPDHLGLIDIQRDRGNGGFIVTNPNCSTIVLVLALAPLR
                     AFEFHDVRAATMQAISGGGFAGVPAMDIYDNVIPYIGSEEEKIETETAKIMGTFNGSE
                     VIPAPFKVSASCHRVPVIDGHTIAVWVDVRESVAEVKKAYATFKSPFSNLPLQPAKAV
                     ELFDQPDRPQPRLDRNRGRGMTVSVGRIREGLRFVALGHNTIRGAAGASVLNAELIHS
                     KKYL"
     gene            4456..4731
                     /gene="albA"
                     /locus_tag="PLI76_09275"
     CDS             4456..4731
                     /gene="albA"
                     /locus_tag="PLI76_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006325 - chromatin organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein Alba"
                     /protein_id="HOI62265.1"
                     /translation="MIKDNTVFVGNKPVMNYVLAVVTQFNNGAEEVAIKARGKAISRA
                     VDTAEIALNRFLENVSKKEIFTSTEMIDTDTGKTNVSSIEIVLMHAK"
     gene            4949..5398
                     /locus_tag="PLI76_09280"
     CDS             4949..5398
                     /locus_tag="PLI76_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62266.1"
                     /translation="MKWVAVGVMLMLVAALVAPAMAAGEERYGYITVKDVTIRLENED
                     AVVTMNYTIDGGIAFLVFLLGKSDLKQKTLDILNFNDTRVQHLDLERIEVRVNNVSDN
                     YGQGSYWFPTHRFGVVVPSLTIITPQDTRHYENISEFPDGFGYFSRP"
BASE COUNT         1200 a         1630 c         1702 g         1035 t
ORIGIN      
        1 ggggggctcg cctgcaatag tcactccgaa ccggtgttgc agggatgacc gccggggagc
       61 accggatacc gggcgggatc cctcacccgc ccccaccggt gcaaaaaccc gcacgcttat
      121 ctctgcgccg gataacccgt ccctcatgga gagagacgaa gcactggccc tgctccggcg
      181 ctacgtggtg tcgccgtcac acatcgcgca cagttacgcc acggcgggca tcatgaagag
      241 ggtcgccgaa cacctcggcg aggatgccgg cacctgggag atcatcggcc ttttgcacga
      301 catcgactac gacctcgtcg ccggagacat ggaccggcac ggcatcgagg gccaccggat
      361 cctcatcgag aacggcgttc ccgaagagat cgcggatatc gtcaggcgcc acaaccacac
      421 ccatttctcc gattacgtcc ggcccgtcga gatcgcgctc caggcggcgg acagcgcatc
      481 gggccttatc atcgcctgcg ccctggtgaa gggcggggcg gtcagcgagg tcgcgccccg
      541 aaccgtgaag aagaagttca aagagaagtc gtttgccgcc gggtgcgagc gcgagaggat
      601 ccggatgatc gaacccctca tggatatgga gacgttcttt cgcctcgcga tcgagggtat
      661 catggatatc cgtaccgata tcggcctcac ctgagcggaa tccttatgag acgcccggca
      721 cgaggagtat agcatgacag gggtagaccg cgcgcgggtg atcgagcgca tgaacgaggc
      781 gatgatgctc ctcaccgagc ggatggatgc ccgcctcata ccggagacgg gtatgaacat
      841 cgcctatgcc ctgcccgacg cgcggagcaa ggacgatgtg gcggcggtcc cgggagggat
      901 cgtcgggcac gccgatcggg tccaccccgc cggcgatatc gcgttcggtg cagacgccta
      961 cgtggcccgg atcgtcatta cagccatgcg gtttgacccg gcgatccgga gcgcggcaac
     1021 catccgcttc tcggaagcgg tccttatcga gatggagaac ctcctcttcg agatatgctc
     1081 gtttgacccg gaaaaagagc cgccgggggt gcagacaatg gattggggtg tcgcctcctg
     1141 ctgcagagag ggcgtgcccg acgtcatcta cgaccggggc gccgcaggga aagaaccgac
     1201 gatcaggctg ctcggggaag atccggtcac ggtcgcacgc aatcttctta agctgtcgaa
     1261 tcgcattacg tatgcacaat tgtaagggaa ggtccacatg ggaataaagc cgtcatatat
     1321 caagaacctc ggcgaggaac tcatcgtcaa gcatcgtgag agatttagcg gagattttga
     1381 ggagaataag cacgccgtct ccgaagtcgc cacgatcggg agcaagtccg tccgaaaccg
     1441 ggttgccggg tacatctcaa gaaagataaa taccaggaag cgttaaacct cactatatgt
     1501 ttgagggaat ccttccagca atcattaccc cttttcatcg ggactcgggg atgagtctcg
     1561 atattgaagg attacggtcc aacatagaat cactgctgca acgcgggatc catggcatcg
     1621 tcccctgcgg atcgaccggg gagtccgcga cgctcacgtt cgaggagcac gagcaggtga
     1681 tctcaaagac cgtcgaggtt gtcaacgggc ggataccggt gcttgcggga accgggtcga
     1741 acaacaccga agaggcaatc cggctgaccc gcgcggcaaa ggatgcaggc gcggacggcg
     1801 cccttgtcat cagcccgtac tataacaagc cgaaccgctc cggtctcgtc aaacacttca
     1861 cgaagcttgc cgatatcggt ctccccatcg tcctctacaa cgtccccggc cggacggggc
     1921 agaacctcct gccggacctg gtgatcgagc ttgcccggca cccaaacatc gtcggcgtga
     1981 aggaggcaag cggcgatatc gtccagatat cccggatcat cgaggggacc cgggacgagg
     2041 agttcgtcgt gctctcgggc gacgacgcga tgacgcttcc cgtcctcgct ctcgggggcg
     2101 cgggcgtgat atcggtgact gcaaacgtcg accccggccg gatggtgcag atgtacgagg
     2161 cgttccgcgc gggcgacctt gcccgcgcac gggacctgca cttcgaactc tcgccgctca
     2221 tgcgggcgat gttcatcgac acgaacccca tccccgtgaa gaaggcggtc gggctctccg
     2281 ggatggccgc aggaccggtc aggctgccgc tcgacgacct ggatgggcag aagacggagc
     2341 agctgaggaa ggtgctggcg agctatgatt aaggtagccg tatccggggc cctggggaga
     2401 atgggcgcca acatcggccg gcttgtcaac gaggctcccg acctcaccct ggtcggcggc
     2461 atcgacgtga gggagggaac gctctttgag acggaggtcg tccccgcagc gaagatcgac
     2521 gagttcctca aggagaagag acccgacgtc ctcgtcgact tcaccgtcgc ggccgctgcc
     2581 gtcgagaacg tcagggccgc cgcgagaaac ggcgtagcgc tcgtcgtcgg aacgacaggg
     2641 ttctctcccg agcagcggga ggtgatccgg ggcgcggtcg aaggaaacgt ccccgccgtg
     2701 atatcgagca acttctccgt cggcgtcaac atcttctgga aactcgtgcg ggaagccgcc
     2761 cgcgagctcg gcgactacga catcgaggtc acggaggccc accaccgcca caagaaggac
     2821 gccccgagcg gcaccgcaaa gaccatcctt gacatactgg acgaggagat cgggaatcgc
     2881 gagaaggcct acggccgtgt gggtatgacg gagcggaaga acgagatcgg ggtgcacgtc
     2941 atccgcggcg gcgacatcgt cggcgaccac gcggtcctct ttgcggggaa ctttgagtgc
     3001 atcgagatct cccaccgcgc ctacgatcgg gcggtcttcg cacagggtgc cgtccgggct
     3061 gtccgctggg tcgtcgggaa agaaccgcgc atgtatacca tgcaggatgt cctcgggatc
     3121 tgagggaggc cccctcgccc gccttccgga aacgaaattt atttttacgc cgctagaaaa
     3181 acatgtacgt ctggaggaga aaaattgcca gcagttgttg atattgagaa gtgtaccgcc
     3241 tgtgagacct gtatagatgt ctgcccggct tccgccatca ccatggaaga cggcaaagca
     3301 aaggtcgatc ccgatctctg cgtcgattgc gagacctgcg tcgacgagtg tccgtctgag
     3361 gcaatctcga tggaatgaag aaacatactc tttaatacct cctacactac agatattttt
     3421 gtctatgatt catgtaggag tgcttggtgc cacaggagcg gtgggacagc gcttcgttca
     3481 gcttttagcg gatcatccct ggtttcatct ccagacactc accgcttctg aacgaagtgc
     3541 gggaaaaccc tatggtaaag tggtcaactg gcgccttgat gcgccgtacc cggacaacgt
     3601 cagcgatatc gtcgtcactc ccacaaccgt cgagagtctg aaagattgcg atctcgtctt
     3661 ctcagcgctc cctgccgacg tggcgggacc gttcgagacc gagcttgcca atgcgggcat
     3721 cgccgtctgc agcaacgccc gatcgcaccg catggaaccg gacgttccgc tggtgatccc
     3781 ggaggtcaat ccggaccacc tgggcctgat cgatatccag cgggaccgcg gtaatggggg
     3841 gttcatcgtg accaacccga actgctcgac gatcgtccta gtgctggccc tcgcacccct
     3901 cagggcgttt gagttccatg acgtgcgtgc ggcaaccatg caggcgatat cgggaggcgg
     3961 gtttgcaggc gtccccgcca tggacatcta tgataacgtc atcccctata tcggctcaga
     4021 agaggagaag atcgagaccg agaccgcaaa gatcatgggc acgttcaacg gttccgaggt
     4081 gatccctgcc ccgtttaagg taagcgcaag ttgccaccgg gtccctgtca tcgacgggca
     4141 taccatcgcg gtctgggtcg acgtcaggga gtcggttgcc gaggtgaaaa aagcttacgc
     4201 aacctttaag tcacccttca gcaatctacc cttacaaccg gcaaaggcag ttgagctctt
     4261 cgaccagccc gatcgtccgc aaccccggct tgaccgcaac cggggacgcg gcatgacggt
     4321 ctccgtcggg agaatccggg aaggattacg gtttgttgca cttgggcata acaccatccg
     4381 tggggctgca ggcgcatccg tcctcaacgc agagctgata cactcgaaga agtaccttta
     4441 gacgaggtgg gcttgatgat taaggataac acagtattcg tgggaaacaa accggtcatg
     4501 aactatgtgc tcgcggtggt cacccagttc aacaacggag cggaggaggt tgcgattaag
     4561 gccaggggga aggctatttc acgtgcggtc gacacagcgg agatcgccct caaccggttc
     4621 ttagagaacg tcagcaagaa agagatcttc acatcgaccg agatgatcga caccgacacc
     4681 ggaaagacga acgtctcaag catcgagatc gttctcatgc atgcaaaatg aggccctgag
     4741 gcacctcttt ttggattcgc ccctgtacgc gcctcggcgg gatcgctcat cgaggcgcct
     4801 cttccctcat cgacgttgcg ctgcagggtg ccggcgcatc gtaaaagcag gttttatgag
     4861 ggcgatccgc ctgaaatttc agccgtacgg tctttttgga ccggggcaac cataaagatt
     4921 ttatgagcca ccccataact attgtaccat gaagtgggtg gcggtcggag tgatgctgat
     4981 gctggtggct gctctcgtcg ctcctgctat ggcggcgggt gaggagcggt acggttacat
     5041 cacggtaaag gatgtcacca tccggctgga gaacgaggat gccgtcgtca ccatgaacta
     5101 tacgatcgac ggcggcatcg cattcctcgt cttcctcctc ggaaagtccg acctgaaaca
     5161 gaagacgctt gatatcctga atttcaacga tacgagggta cagcatctcg acctcgagcg
     5221 catcgaggta cgcgtaaaca acgtctcgga taattacggg cagggatcgt actggttccc
     5281 tacgcaccgg ttcggtgtgg tcgttccctc tctcaccatc atcaccccgc aggacaccag
     5341 gcattacgag aatatcagcg agttccctga cgggtttggc tatttttccc gaccgtgaac
     5401 ggacccggct tatcctatga gagaggcacg atgtgcctga cgaccactgt ctccgccttc
     5461 cgggagccgt gcaggcgatc cggccacgcg aatgtatgct gcttgctcaa gagcagatag
     5521 ggccctgcag gtctcgcgag ggtttcggga aggggcggat gagagcg
//
LOCUS       DAOSEE010000135         5467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_5335_length_5467_cov_256.590355, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000135 DAOSEE010000000
VERSION     DAOSEE010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5467
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5335_length_5467_cov_256.590355"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="PLI76_09285"
     CDS             <1..417
                     /locus_tag="PLI76_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase maturation
                     protein"
                     /protein_id="HOI62267.1"
                     /translation="NALLTECIGTLERGYRFVVVDSEAGMEHIARGTIKNPDVLLIVS
                     DPSARGIRTAGRIRDLAEGLGLSRDRVFLAVNRDRGDAVVESNLPLVARIPEDRGVGE
                     ADLAGTPIVGIPQESPARAAVRMLAGWLVEECARRG"
     gene            complement(601..990)
                     /locus_tag="PLI76_09290"
     CDS             complement(601..990)
                     /locus_tag="PLI76_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOI62268.1"
                     /translation="MENQSIARLMEEGAAFFSAGEAGRRLTWYTNPAWTGVSLADLAP
                     GADTKGSFTSHFVRIRKGCEVPDHLHESQWEWNVILDGHAKMVLDGREIPFKPGDTFT
                     TPPGVRHAVVAGKEDVALLAVFVPGPA"
     gene            complement(1031..2023)
                     /locus_tag="PLI76_09295"
     CDS             complement(1031..2023)
                     /locus_tag="PLI76_09295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HOI62269.1"
                     /translation="MKTQIGYFASLEQYRPMDALEQTIRAEKVGFDSIWADDHFHPWY
                     HDNAQSAQAWAWMGAALQATKRVFISTCITCPIIRYNPAIVAQTFATLRQMYPKRVGV
                     AVGAGEAMNEVPVTGVWPSVAERQDMTVEAIEVMRKLWESKEPVTFKGKFFTLDKAFM
                     YTKPDDEVPLYFSGLGPKGAKLAGMYGDHLMTVAAAPSVLKNVTIPRFEEGAREAGKD
                     PSKMERAMLIWYSVDPDYEKAVEGNRFWAGCLVPSMFKYKVYDPKEVQLHANLVHCDT
                     IKENYMCATDAEEMIKEIERFKAAGINHFCLGNSSPDVNTGIDIFKEIIPAVRD"
     gene            complement(2685..4100)
                     /locus_tag="PLI76_09300"
     CDS             complement(2685..4100)
                     /locus_tag="PLI76_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOI62270.1"
                     /translation="MSDYQDKFSKIVDLLEERSPQGLSISAIARELGMNRASVSKYLE
                     MLQSSGDVSMQRFGRSKLYTPAHQVPFSELFDRLSSAIVILDADLRILMVNTSFIKAL
                     GIHRERNIIGAPLFDLNLRILSDPAIRRNIERIRAAGPYLAEMQHIEDSTNHIHLIEF
                     APIASRAGKPGIMISLRDITAWKNAEVALKNSEKKIRTIFETAPSGIVFFAVDGTILN
                     ANRASLRILGLKDFQELVNGNIFDISCYKGKILELIRQGKVAETELVCDFDLLKQEQR
                     IPSSRSGIAYFDVVFTPIALDGGGAPREFAILFWDVTTERLTRKELVFKETRYRSFFE
                     NTCNGVLIYEPVDDGNEYIFKDINHATEQILRMKKEDLIGRKLFEVFPDLPDPDVRNA
                     LIRVLKTEKPEFLLPLQYRKGKDSPWIWHYIFKLPSGEIASFMIDVSDAIREDAEGPL
                     RACGMHRQHSRDQPAPVSHAD"
     gene            complement(4411..4578)
                     /locus_tag="PLI76_09305"
     CDS             complement(4411..4578)
                     /locus_tag="PLI76_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62271.1"
                     /translation="MRQGITLLLALVAGATAAGEVAPPAFPHEFYGSMTIDGATASPP
                     LKSHDEDRDRR"
     gene            complement(4589..>5467)
                     /locus_tag="PLI76_09310"
     CDS             complement(4589..>5467)
                     /locus_tag="PLI76_09310"
                     /inference="COORDINATES: protein motif:HMM:NF023252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3344 domain-containing protein"
                     /protein_id="HOI62272.1"
                     /translation="TAPHTVLVNEGFDLLYGGAAQGTTPEQATAYAPFAIDAKDAVNA
                     RLVTVAPGAGPTKGDLLFNDRVWTRVWNYTGTTQIGVDDRDVTALLAGENLAGFRSDA
                     DYMEAAAAFLVVEYPVPAGDTAIILSPGWNFVSTPKRLADGANTFAIFDTVDTAAHSI
                     QLYDGLERKWRQMAPTDPFRPLDAVWVYANKTCTVPLTFAAGTPELPPAKDLGKGWNA
                     IGFTDTVPETANYTLQSLGDHWSTLIGFDAGIQEYDTSIVRGATGPHGDERLMQPMQG
                     YWLYVNAADTLGSLSG"
BASE COUNT         1041 a         1588 c         1698 g         1140 t
ORIGIN      
        1 aacgccctcc tcaccgagtg catcgggacg ctcgaacgcg gctaccggtt cgtcgtcgtg
       61 gactccgagg ccggtatgga gcatatcgcc cggggaacga tcaagaaccc ggacgtcctc
      121 ctgatcgtga gcgatccctc tgcccgcggt atacgaaccg ccggccgcat ccgcgacctc
      181 gcggaggggc tcggcctctc gcgcgaccgg gtattcctcg ccgtcaaccg tgaccggggc
      241 gatgccgtcg tagagagcaa tcttcccctc gtcgcccgga taccggagga ccggggcgta
      301 ggggaggcgg atcttgcggg gacgccgatc gtcggtatcc cgcaggagag tccggcacgg
      361 gcggcggtga ggatgctcgc cggatggctc gtcgaggagt gcgcccggcg cgggtgatgc
      421 tccaatataa aaaaggatga ggcggatatt gaaggtgccc cggggttcca tcggcccata
      481 tggggaaggt gccgatcttt cagcggggag atacagggag tggggagggg atccccgcac
      541 ccggatgcac cggacgcgga ggtgtggtat cggggagaat gaggcggaag acccctgccg
      601 tcatgccgga cccggaacaa ataccgcaag cagcgcgaca tcctccttgc cggcaacgac
      661 ggcgtgacgg acaccgggcg gcgtggtgaa ggtgtctccg ggcttaaagg ggatctccct
      721 cccgtcgagg accatcttcg cgtggccgtc caggatgacg ttccactccc actggctctc
      781 gtggagatgg tcgggaacct cgcagccttt cctaatcctg acgaagtggg aggtgaacga
      841 cccctttgta tccgctcccg gcgcaagatc cgccagggag acgccggtcc acgccggatt
      901 cgtataccac gtcaaccgtc tgcccgcttc cccggcggag aagaaggcgg caccctcctc
      961 catgagccgg gcaatgctct ggttttccat cgttcctcag ggaaatgggg atggaccggt
     1021 gatgcccggt tcaatccctg acggcaggga tgatctcctt gaagatatcg atgccggtgt
     1081 tcacatccgg gctcgagttg ccgaggcaga agtggttgat gcctgccgcc ttgaaccgct
     1141 cgatctcctt gatcatctct tcggcatccg tggcacacat gtagttctcc ttgatggtgt
     1201 cacagtggac gaggtttgcg tggagctgga cttccttcgg gtcgtagacc ttgtacttga
     1261 acatcgaggg gacaaggcat cccgcccaga accggttgcc ttcgaccgcc ttctcgtagt
     1321 cggggtcgac cgagtaccag atgagcatcg cgcgctccat cttgctcggg tccttcccgg
     1381 cctcgcgggc gccttcctcg aacctgggga tggtgacgtt cttgagaacc gacggggcgg
     1441 ctgcaacggt catgaggtgg tcgccgtaca tgccggcgag tttcgcaccc ttggggccga
     1501 ggccgctgaa gtagagcggg acctcatcgt cgggtttggt gtacatgaac gccttgtcaa
     1561 gagtgaagaa cttgcccttg aaggtgacgg gctccttgct ctcccagagc ttccgcatca
     1621 cctcgatagc ttcaacggtc atgtcctgcc gttcggcaac gctcggccat acgccggtca
     1681 ccggcacttc gttcatcgcc tcgccggcac ccacggcaac gccgactctc ttcgggtaca
     1741 tctgccgaag ggtggcaaac gtctgcgcga cgattgccgg gttgtagcgt atgatcgggc
     1801 aggtgataca ggtcgagatg aatactctct tggttgcctg gagcgctgca cccatccagg
     1861 cccaggcctg cgccgactgc gcattgtcgt ggtaccaggg atggaagtgg tcgtccgccc
     1921 atatcgagtc gaagccgacc ttttctgctc tgattgtttg ttcaagcgca tccatggggc
     1981 gatactgctc cagagatgca aagtaaccga tctgtgtctt catggtactg gtctcctgtc
     2041 gatcggcaga gtaccctatc cggaggcaag actaccctaa tcttgatatg ttaccagcgc
     2101 caatgaaaac actggtacgg cagctgaggc ccgcacgtca atcccacttg cctgtagcgg
     2161 accaaggcca tcctaccccg ttaatggttc tcttccgatt ctgtcgtatg cggactggtc
     2221 gtccacataa gaagtaatat atctaactgt agatataggt gttgtttatt gattaacaat
     2281 aactccggca attatacggg atatcaggcg gtgcggcacc cgcccctgga ttaaaattgc
     2341 agtgattttc tcaattattg aataaatgtg aaatataaca tcctttctgg catcgtgtgt
     2401 actgtggcat gcggataggc cggcaggcgc cggcgcggtg caacgttaat caataaacaa
     2461 tatattttta tattgaaacc ataagttaac tccattgggt tatttgttcg cgcgaatagg
     2521 gcaggagcga gaaggccccg gaagaaggcc ggagggggga tcggcattct ccttcccata
     2581 tactgccgct tcccggacag cgtttcacac acgaccgcct ttccagcatc ggcgttcggg
     2641 catgggtccc gcctccggat acgctgtcca ggcgccccca tgaatcaatc ggcgtgggac
     2701 accggagccg gttgatcccg ggaatgctgc ctatgcatac cgcatgcccg gagcggccct
     2761 tcagcatcct cgcgaatagc atcggagaca tcgatcatga acgatgcgat ctccccggag
     2821 ggtagtttga atatatagtg ccagatccag ggagaatcct tccctttccg atactggagg
     2881 ggcagcagga actcaggctt ttcggttttc aagacccgga taagagcgtt ccgaacatcg
     2941 gggtcgggga gatcggggaa gacctcgaag agttttctcc cgatcagatc ttccttcttc
     3001 atccggagga tctgctctgt agcatggttg atatccttga agatatactc gttgccatcg
     3061 tcgaccggtt cgtagatcag gacaccgttg caggtattct cgaagaacga tcggtagcgg
     3121 gtttctttga acaccagctc tttccgggtc aaccgctccg tggtgacatc ccagaagagt
     3181 atggcaaact cgcgcggggc tcccccgcca tcgagggcga taggggtgaa gacaacatca
     3241 aagtaggcga tgcccgatcg ggagctcggg attcgctgtt cctgcttcag gaggtcgaaa
     3301 tcacagacga gttccgtctc ggcgaccttt ccctgccgga tcagttcaag aatcttccct
     3361 ttatagcagg agatatcgaa gatgttcccg ttcaccagct cctgaaaatc cttcagaccg
     3421 aggatccgca gggacgcgcg gtttgcgttg agaatggtgc cgtcgaccgc aaaaaagacg
     3481 atcccgctcg gggccgtctc gaagatcgtc cggatcttct tttctgagtt cttcagggcc
     3541 acctcggcat tcttccatgc cgtgatatcc cggaggctga tcatgatccc gggctttcct
     3601 gcgcgggatg cgatcggggc gaactcgatg agatggatat ggttcgtgct atcctcaatg
     3661 tgctgcatct cggcaagata cggtccggca gcccggatcc tctcgatatt tcgcctgatc
     3721 gccggatctg agaggatgcg gaggtttaag tcaaagagcg gcgcaccgat gatgtttcgc
     3781 tcgcggtgga tgccgagggc cttgatgaaa cttgtgttta ccatgagaat gcgaagatcc
     3841 gcatccagga tgacgatcgc gctggagaga cggtcaaaga gctccgagaa gggaacctga
     3901 tgcgccggtg tatataattt tgatctgccg aaacgctgca tgctcacatc cccgcttgat
     3961 tgaagcatct cgaggtactt ggagacggaa gcacggttca tcccgagttc gcgggcgatc
     4021 gcagagatag agagtccttg aggcgaccgc tcctcaagaa gatcgacgat cttgctgaat
     4081 ttgtcctggt aatcgctcat gggtgctgtc ccccgaaccc cgccgggttt ccctgagtat
     4141 ccacggtcgg gatctgtact ggtaatttgg ccggatcggt aattttacct ttcgggcccc
     4201 gaaggaatcc ccaccctgcc ggcaccggag caccggtttc gtcccgggca tggccctcgg
     4261 gaagcccgat cgacggaagc gtcacctgca tcgacttccg tatcggggcg ccggataatc
     4321 ccgatgctgc cgcggccagc caccgggagt gcaggagaaa cgactgcccg ggcctcctgc
     4381 cggcgggatc gtggaggcgc tcgccggctg ctacctgcga tcccggtcct catcatggct
     4441 tttgagggga ggcgaggctg ttgcaccgtc gatcgtcatg ctcccgtaga actcgtgggg
     4501 gaaggcagga ggtgccacct ccccggccgc cgtagcgccg gcgacgaggg cgaggaggag
     4561 ggtgatgccc tgtcgcacgc ctttcctctc acccgctgag actgccgagc gtatctgccg
     4621 cgttcacgta cagccagtag ccctgcatgg gttgcatcag gcgctcatcc ccatggggac
     4681 cggtggcgcc gcggacgatc gatgtatcgt actcctggat gcctgcatca aacccgatca
     4741 gggtggacca gtgatcgccg agagactgca gggtgtagtt cgccgtctca gggacggtat
     4801 cggtgaaccc gatcgcgttc cagcctttcc cgaggtcttt tgcgggcgga agctctggtg
     4861 tgccggcggc gaaggtcagg ggaacggtgc aggtcttgtt tgcgtagacc cagaccgcat
     4921 cgagcggccg gaacgggtcg gtcggagcca tctgcctcca cttccgctcc agaccgtcgt
     4981 agagctggat cgagtgggcg gcggtatcga ccgtatcgaa tatggcgaag gtgttcgcac
     5041 cgtcagcaag cctcttgggc gtcgaaacga agttccagcc cggggagagg atgatcgcgg
     5101 tatccccggc cgggaccggg tactcgacga ccaggaaggc tgccgcagcc tccatgtagt
     5161 cggcgtcgct ccggaacccg gccaggttct cgccggcaag gagcgccgtc acgtcgcggt
     5221 cgtcaacccc gatctgggtc gtgccggtgt agttccagac gcgggtccag acccggtcat
     5281 tgaagaggag gtcgcccttt gtcggcccgg caccgggggc gacggtgacc aggcgggcgt
     5341 tgacggcgtc cttcgcatcg atggcgaagg gggcatacgc cgtcgcctgc tcgggggttg
     5401 tcccctgggc cgcgccgccg tagaggaggt cgaagccctc gttcaccagc accgtgtgcg
     5461 gggcggt
//
LOCUS       DAOSEE010000136         5314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_5511_length_5314_cov_260.132154, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000136 DAOSEE010000000
VERSION     DAOSEE010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5314
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5511_length_5314_cov_260.132154"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2256
                     /locus_tag="PLI76_09315"
     CDS             <1..2256
                     /locus_tag="PLI76_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrophobe/amphiphile efflux-3 (HAE3) family
                     transporter"
                     /protein_id="HOI62273.1"
                     /translation="MKNPYQWLADAINNHTWAVAGVAGAIFILALFGLSMVTMETGDD
                     TYIDKNTPRGALLAHYKDTYGSDAIMLIYEADSVRSPDALRYIDHLQEDLRNERYVDS
                     VSGVVDLLKQANGGTLPSSKAEIDAIIGEAPPEMVERMLPSDLMTISVITLDPGVSMS
                     AQKQVLGNIESTIRISGPPPGLSVTVSGNPAFSKEMEGEMGGQMGILILAAMLLMVLA
                     VLLLFSHVRYSLLPVGVVAVGLIMTFGFMGLSGISISMVVIGAFPVLIGIGIDYAIQF
                     HARFDEEIQRGTIPEAVWATVVNLGPSVLIAMSATALGFIAMFFAPVPMVADFGMVCT
                     IGVASCYIAALIIVPVFATVMNYRPKKEAPTSGSQEGGLSFIERYDRLLGRLAYTVAK
                     HPIPVILLVAVVAAGGYHLDQSVPISADEKTFVPDDMPALRDLEKITRTMGETSSIPV
                     VVIADDVLSPETLAWIDQFGVYEQEHNDKITGVASIATLIREHNGGVLPSTRGEVTDV
                     VARIPEPTLKHYLNGNMEAVLEFSTVAMEMSVARDLVKRMQSDATWNNPPAGVTVKIT
                     GTMEMFAAVMDDIAESRTFMTLLGFAFIIGFMILVYRKFRAISPVIPIVFIVGWNGAI
                     MYLLGLDYTPLTAVLGSMTIGVASEYTILIMERCEEELARGMEFLDAIQIAVQKIGTA
                     ITASGMTTVFGFAALTLSTFNIISNFGIVTVITVGFSLLGAIVVMPAVLSLMYRYTGR
                     TQAPAAIGAAE"
     gene            2401..3093
                     /locus_tag="PLI76_09320"
     CDS             2401..3093
                     /locus_tag="PLI76_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62274.1"
                     /translation="MANEDLAAQVLAPFSALQELINSIILFLPSIIAAIVILIIGWIL
                     GRVLGRIVAEVLDRIGVEGALRKTSVGAYIEQSGTNIVKLSELIVRWIIYIIAILAAI
                     SVLHIPSLTIAVGIIAAYIPNVIAFILILVIGIILVDWLMDFLEDMGEKQRIEGLGVV
                     TLALRAFFYFIIVILALQQLLIDLSIIYVFVIPLAWGVALGLGAAIAIIVGFGLRDRA
                     PEIMDRLIGRVR"
     gene            complement(3119..3892)
                     /locus_tag="PLI76_09325"
     CDS             complement(3119..3892)
                     /locus_tag="PLI76_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOI62275.1"
                     /translation="MNHDLKAAVLARCRSMEIPLVGVASTDRWENPPFKPWMPEEFYP
                     QSIFPEARSVIVIGLPVHLPVLRTAPSIYYHELYRTINTLLDQYTYRLASFLNDRGYP
                     SVFIPRDGYGGIDVLLKNPVAFFSHRHAAFLAGLGTFGVNNALITPEYGPRVRFGSIL
                     TAAPLPPDPLREEGLCTRCMECVTACPAGALDGRDYPEGLTDKAACARNSADLARRHI
                     SPCGICIQVCPVGEDRRHYGGEDSRHCRAREHVQKYGGL"
     gene            4172..4438
                     /locus_tag="PLI76_09330"
     CDS             4172..4438
                     /locus_tag="PLI76_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62276.1"
                     /translation="MSEAKGHFERGRWVVDPVPAPEPEKEPAPKTPIVEERTHEAPKP
                     VQKAGSHPPDAHEQIELAARKAGEDLERAINAWAASARQGLQKG"
     gene            complement(4445..5275)
                     /locus_tag="PLI76_09335"
     CDS             complement(4445..5275)
                     /locus_tag="PLI76_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOI62277.1"
                     /translation="MTNRRYPVVLVHGWRSHPGIWNRLTPVLSDAAVPFWLFDYAAMQ
                     NAGTEAIALALQDYIHTKREETGYSGPVDVVCHSMGTCIARYLLEVLDGGTREEGVRQ
                     LVGIGPPNNGSAMAELFNDPLRGPEVIRSLAGVFVPPDYDPGDDTIVQEFRPRSRIVA
                     ALRAAGTRDDIAYRIVLAVNLTRTPAFFPIFDGRTWEIAPDREWRATYAGDGIVPHID
                     SHLPGVRTDVIPADPVNFARHPGQYCHIMLPRNPEVVARIMEYLCSPDAGPAGGSPVP
                     "
BASE COUNT          980 a         1660 c         1667 g         1007 t
ORIGIN      
        1 atgaagaacc cctaccaatg gctcgccgac gccatcaaca accacacctg ggccgtcgcc
       61 ggggttgccg gagccatctt catcctcgcg ctcttcgggc tctcgatggt gacgatggag
      121 accggcgacg acacctacat cgacaagaac accccccgcg gcgcgctcct cgcccactat
      181 aaggacacct acggctcaga cgcgatcatg ctcatatacg aggcggacag cgtccggagc
      241 cccgatgccc tcaggtatat cgatcacctc caggaggacc tcaggaacga gcgctacgtc
      301 gacagcgtct ccggcgtcgt cgacctcttg aaacaggcaa acggcggcac cctgccgtcg
      361 tctaaagcgg agatcgacgc tatcatcgga gaggctccgc cggagatggt cgaacggatg
      421 ttgccttcag acctcatgac gatcagcgtc atcaccctcg accctggcgt ctccatgagt
      481 gcccagaaac aggtcctcgg gaacatcgag tccaccatcc gcatatcagg gcccccgccg
      541 ggcctctcgg tcacggtctc gggcaacccc gcgttctcca aggagatgga gggggagatg
      601 ggaggacaga tgggcatcct gatccttgcg gccatgcttc tcatggtcct tgcggtgctg
      661 ctcctcttct cccacgtccg ttacagtctc ctccccgtcg gggttgtcgc cgtcgggctc
      721 atcatgacct tcgggttcat gggcctctcc ggtatttcca tcagcatggt ggtgatcggg
      781 gcgttcccgg tgctcatcgg tatcggtatc gactacgcca tccagtttca cgcgcggttc
      841 gatgaggaga tccagcgcgg cacgattccg gaggccgtat gggccacggt ggtgaacctc
      901 ggcccctcgg tcctgatcgc gatgtcggcg acggcgctcg ggttcatcgc catgttcttt
      961 gcgccggtac cgatggtggc cgacttcggt atggtctgca ccatcggggt cgcgtcctgc
     1021 tacatcgcgg cgctcatcat cgtcccggtc tttgcaaccg tcatgaacta ccggccgaag
     1081 aaagaggcgc cgacatcagg ttctcaggaa ggcggactct cgttcatcga acggtatgac
     1141 cgcctcctcg gcaggctggc ctacaccgtc gccaaacacc cgatccctgt catcctgctc
     1201 gttgccgttg tcgcggcagg ggggtatcat ctcgaccaga gcgtccccat cagcgcagac
     1261 gaaaagacct tcgtccccga cgatatgccg gcacttcggg atctcgaaaa gatcacccgg
     1321 accatggggg agacgagctc catcccagtc gtcgtgatcg ccgatgatgt cctctccccg
     1381 gagacacttg cctggatcga ccagttcggg gtctacgaac aggagcacaa cgacaagata
     1441 accggtgtgg cgagcatcgc aaccctgatc cgggagcata acggcggggt gctgccctcg
     1501 acccgggggg aggtcactga tgtcgtcgcc cggatccccg aaccgaccct gaaacactat
     1561 ctcaacggga acatggaggc ggtgcttgag ttctcgacgg tcgcgatgga gatgagcgtc
     1621 gcccgcgatc tcgtcaagag gatgcagagc gatgcgacat ggaacaaccc gccggccggc
     1681 gtgacggtga agatcaccgg caccatggag atgtttgccg ccgtcatgga cgacatcgcc
     1741 gaatccagga ccttcatgac cctgctcggg ttcgctttca tcatcgggtt catgatcctc
     1801 gtctaccgga aattcagggc catatcgccg gtcatcccga tcgtcttcat cgtgggctgg
     1861 aacggggcga tcatgtacct cctcggcctt gactacaccc cgttgaccgc cgtcctcggg
     1921 tcgatgacga tcggggtcgc atccgagtac accatcctga tcatggagcg gtgcgaggag
     1981 gaactcgcac gaggcatgga gttcctcgac gctatccaga tcgccgttca gaagatcggg
     2041 acggccatca cggcatcggg gatgacgacg gtcttcgggt tcgcggcgct caccctctcg
     2101 acgttcaata tcatatccaa cttcggtatc gtgacggtca tcaccgtcgg gttctcgctt
     2161 ctgggtgcga tcgtcgtgat gccggcggtc ctctctctga tgtaccgcta caccgggcgg
     2221 acacaggccc cggcggcgat aggggctgcg gagtaacccc tcccatcgcc cctcgttccc
     2281 cgggggttgt cgcgaccggg atggacgcct ccgctaacca ccgcccctcg ccctccgggg
     2341 aaggcagggc atatcaaggc atatatagta cctgctatat ccctccaccg gtggtatacc
     2401 atggcaaatg aagaccttgc ggcgcaggta cttgcgccat tctcagctct ccaggagtta
     2461 atcaacagca tcattctgtt tctgccgagt atcatcgcgg caatcgtcat tctcatcatc
     2521 ggctggattc tggggcgagt tctcgggagg atcgtcgccg aggttctcga ccgtatcggc
     2581 gttgaaggcg ccctccggaa gacgtcggtc ggagcctata tcgagcagtc ggggacgaat
     2641 atcgttaagc tatccgaact catcgtccgc tggatcatct acatcatcgc gatccttgcg
     2701 gctatcagcg tcctccatat cccatccctg acaatagcgg tcggtatcat tgcggcctac
     2761 atcccgaacg tcatcgcgtt catcctcatc ctggtcatcg ggatcatcct ggtcgactgg
     2821 ctcatggatt tccttgagga tatgggcgag aaacagagga tcgaggggct tggggtggtt
     2881 acactggcgc tccgggcgtt cttttacttc atcatcgtca tactcgcact ccagcagctc
     2941 ctgatcgacc tctcgatcat ctacgtcttt gtcatcccgc ttgcctgggg agttgcgctc
     3001 gggctcggtg cggcgatcgc catcatcgtc gggttcggcc tccgggaccg cgcgccggag
     3061 atcatggatc ggcttattgg ccgggtaaga tagacataac cctttttccg ggcgggtctc
     3121 acaacccgcc gtacttctgc acgtgttcgc gggcccggca gtgccgggag tcctcgccgc
     3181 catagtgccg ccggtcctct cccaccgggc agacctgtat gcagataccg cagggagata
     3241 tatgtcgtcg ggcgaggtcg gcgctgttcc tggcacaggc agccttatcg gtgaggcctt
     3301 cggggtagtc ccggccgtca agcgcgcccg cggggcaggc ggtgacgcat tccatgcacc
     3361 gggtgcagag gccttcttcc cgcaaggggt cgggcgggag gggtgcggcg gtgaggattg
     3421 agccgaacct gacccgggga ccgtattcgg gcgtgatgag ggcgttgttc acaccaaacg
     3481 tcccgaggcc ggcgaggaag gcggcgtgcc ggtgcgagaa gaaggcaacc gggttcttca
     3541 ggaggacatc gatgcccccg tagccgtcac gggggatgaa gaccgaaggg tagccgcggt
     3601 cgttaaggaa cgacgcaaga cggtaggtgt actggtcgag cagtgtgttg atcgtcctgt
     3661 atagctcgtg gtagtatatc gagggggcgg tcctgagcac cgggaggtgg accggaaggc
     3721 cgatgacgat caccgatcgg gcctcgggga atatcgactg cgggtagaat tcttccggca
     3781 tccagggttt aaacggcggg ttctcccacc gatccgtgct cgcgaccccg acgagcggga
     3841 tctccatact ccggcaccgc gcgaggactg ccgccttcag gtcgtggttc ataactactg
     3901 gtaccctgcc ccggcgaaaa aagattctcc ggtcaggtgc cggccgggaa cggtcccact
     3961 gccagaggat caggcaggtg aaccgcgatc acccggccgg ctctcgtgcg gaggtggaag
     4021 gcgccccccg agtatcggaa gagtcgcgcg acgataccgg agttatccgc cagtcagggg
     4081 ggtttggtcc cctcttcgac cgggtgcatc cctctcggtc cttacggtgc aaaaagccag
     4141 gtttttattc ccggcggatc catcaccgca tatgagtgag gcaaaaggcc actttgagcg
     4201 gggacgatgg gtcgtcgacc cggtacccgc tcctgaaccg gagaaagagc cggcaccaaa
     4261 aacaccgatc gttgaagaac gcacgcacga ggccccaaaa ccagtccaga aagcaggcag
     4321 ccacccgccc gatgcgcacg agcagataga actggcggca cggaaggccg gggaggacct
     4381 cgaacgggcg atcaacgcct gggcggcgtc tgcgcggcag ggtttgcaga aagggtgaac
     4441 gggttcacgg taccggggag ccccccgcag gcccggcgtc cgggctgcag aggtactcca
     4501 tgatccgcgc gacgacctca gggttcctcg gcagcatgat gtggcagtac tgccccgggt
     4561 gccgggcaaa gttcaccggg tctgccggta tcacgtccgt ccgcaccccg ggcaggtgag
     4621 agtcgatatg ggggacgatg ccgtccccgg cgtaggtcgc ccgccactct ctatccggag
     4681 cgatctccca ggtccggccg tcgaagatcg gaaagaacgc cggggtccgg gtgaggttca
     4741 cggcgaggac gatccggtag gcgatgtcgt cgcgggtgcc tgcggcccgg agcgccgcga
     4801 cgatccgact ccggggccgg aactcctgaa cgatggtgtc atcgccggga tcgtagtccg
     4861 gcggcacgaa gacacctgca agactccgga tcacctccgg gccccgcagg ggatcgttga
     4921 agagttcggc catggccgag ccgttgttcg ggggaccgat cccgacgagc tgccggacgc
     4981 cctcttctcg tgtgccgccg tcgaggacct cgaggaggta gcgggcgatg catgtcccca
     5041 tcgagtggca gacgacgtcg accgggccgc tgtagccggt ctcctcccgt tttgtatgga
     5101 tgtagtcctg gagagcaagg gcgatcgctt ccgtcccggc gttctgcatc gcggcatagt
     5161 caaacagcca gaagggcacc gcggcatccg agagcacggg ggtgaggcgg ttccatatgc
     5221 cgggatgact ccgccagcca tggacaagga cgaccggata tcgccggttc gtcaggatgg
     5281 cggcatgccc cctctgcggg tacagcggtt catt
//
LOCUS       DAOSEE010000137         5242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_5610_length_5242_cov_189.312319, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000137 DAOSEE010000000
VERSION     DAOSEE010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5242
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5610_length_5242_cov_189.312319"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PLI76_09340"
     CDS             <1..285
                     /locus_tag="PLI76_09340"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HOI62278.1"
                     /translation="TVVNLTAQNIAFDKSTITVPAGANVTVHFTNKDSGVQHNFAVYD
                     SNLRSKSFFVGEIITGPAEANYTFTAPSDPGTYYFQCDIHPFMNGKFIAE"
     gene            422..766
                     /locus_tag="PLI76_09345"
     CDS             422..766
                     /locus_tag="PLI76_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62279.1"
                     /translation="MVGTKKEVKIARILITVSVVFGFLVVVTLLVIGTGGFAGRTGPS
                     GISTTGALLLAAVKAAGTVFGLAAYLTIARGDLRKAGRYALVASILPPFDLIALFAGL
                     LALFSHREGGAG"
     gene            766..1851
                     /locus_tag="PLI76_09350"
     CDS             766..1851
                     /locus_tag="PLI76_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOI62280.1"
                     /translation="MERVLDAGGGPRIVSWNVTARCNLACAHCYIDADGEGSPGELDT
                     AEGMALIDQIAAVGRPILVLSGGEPLLRPDIFDLARYAAGRGLRVAMGTNGILVTDAV
                     AERLSDTGIRKVAVSIDSTDPGVHDAFRGVSGAWEKAVNGIRALRRAGVPVQIHTTIG
                     EPDIGEIDRIIAFGKSHGVRDYQFFFPVPTGRGEDLAGLPPEVHEAVIGRILAHAREP
                     GFSVRPTCAPQFVRVAARMGLDLSQWGRGCIAGTAYCRITPVGDVTPCPYLPVPVGNI
                     RREAFSDIWHGSEIFAALRDPDSLGGKCGQCEYRRACGGCRARAYGVRHRRGDACGGP
                     VRPGDPDGDILAEDPCCPYEPGEITHD"
     gene            1844..2344
                     /locus_tag="PLI76_09355"
     CDS             1844..2344
                     /locus_tag="PLI76_09355"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HOI62281.1"
                     /translation="MTDRHLDILDRQLLEILQDTFPLAPRPWHAIGRRLGIPEGEVLR
                     RVRRLADEGIVLTVSPVIESARIGPVASTLIGMRVPADRVERCASIVNREPGVSHNYL
                     RDHDYNLWFTLAAPDSAALRRTVEDIAARAGVPPADLLDLPAVRRFKIGVRFRFFPDG
                     DGEGGI"
     gene            2341..2820
                     /locus_tag="PLI76_09360"
     CDS             2341..2820
                     /locus_tag="PLI76_09360"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOI62282.1"
                     /translation="MNDRTLLIRLLSGVPLVEEPYRAIAEELGVTEAEVIARIESLLS
                     QGIIRRFAVRLDHRRAGIRVNAMVAWRVPPGDMVQVAEIMARSPEVTHCYERATVPGR
                     WDYNLYAVLHGRRRAGVDRSIARLSRAAGIDDCAVLVSTRELKRTPPGLALLAKEGR"
     gene            2821..3975
                     /locus_tag="PLI76_09365"
     CDS             2821..3975
                     /locus_tag="PLI76_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOI62283.1"
                     /translation="MIRFTHLIHGEGPVSGPAAKHSHLAFEEGRRGPVVFWNITSRCN
                     LACTHCYLRSGPRRRREDELTTGEAKALIDDLAAANVPLLLFSGGEPLMREDFWSLAA
                     HAQERGIPAVLSTNGTLITPTVARRLRDAGIGYAGISLDGATAATHDLFRGVVGSFDR
                     SVQALKYCIDAGLRCGVRFTVTKRNHSELGRLIALARSIGVHRFCVYWLVPSGRGGDA
                     HADLQVGPGEVQGVIDLIYRETLRTDPAAMEFLTVDAPQDGVCLLEMLKADAHPAYDR
                     TYRLLARMGGCSAGRRVANIDPSGNVYPCQFAQFREFLAGNIRDRPFSEIWSDPGNQV
                     LTAFREGEARPGSPCSTCDRQEVCGTGCRVRAYVRYGDLNSGDPLCLRHG"
     gene            4025..4627
                     /locus_tag="PLI76_09370"
     CDS             4025..4627
                     /locus_tag="PLI76_09370"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HOI62284.1"
                     /translation="MRPNVILIVALLVVSAGVLAAGCTTPGENVTQPTAPPTGTETPA
                     VTATTVAETPAATGTTTAETPAATATTAAETPTTAETTAATTTTAATNATTGATTAAG
                     GGTATVDLTAQNIAFDKSTITVPAGAEVTVNFDNQDSGIPHNFAVYTDSSASQSIFKG
                     DTITGPATTTYTFTAPDEAGTYYFRCDVHPEQMNGDFVVQ"
     gene            4986..>5242
                     /locus_tag="PLI76_09375"
     CDS             4986..>5242
                     /locus_tag="PLI76_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62285.1"
                     /translation="MTTPAGGASSAPDEHDSKGDTRKIIGILAALAIFIIVLYGYIIP
                     LQGGFISSTAIRADDLLGADPRFEEQMPIQEVDLGASGRSI"
BASE COUNT          918 a         1776 c         1688 g          860 t
ORIGIN      
        1 acggtggtga acctcaccgc ccagaacatc gcctttgaca aaagcaccat cacggtaccc
       61 gccggggcga acgtgaccgt gcacttcacg aacaaggact cgggcgtcca gcacaacttc
      121 gcggtctacg acagcaacct ccgttccaaa tccttcttcg tgggcgagat catcaccggg
      181 cccgccgagg cgaactacac gtttacggca ccatccgatc ccgggaccta ctacttccag
      241 tgcgacattc accccttcat gaacgggaaa tttatcgcag agtgatggtc ggcatgtgaa
      301 aaaggggcta actgccccat ctctcctccc ttcaggccgg ggtttttggt aacgaacaac
      361 catcccgatc actcaaccgg ccggggaaag gttaatggtc acgacagggg aactgcccgg
      421 catggtaggc acgaaaaaag aggtgaagat cgcccgaatc ctcatcaccg tatcggtggt
      481 cttcgggttc ctggtggtgg tgacgctcct tgttatcggc acgggcggct ttgcgggaag
      541 gaccggcccc tccggcatat ccacgacggg ggcgcttctc cttgccgcag tcaaggccgc
      601 cggaaccgtg tttggtcttg cggcatacct gacgatcgcc cggggcgacc tccggaaggc
      661 cgggcggtat gccctcgtcg caagcatcct cccgcctttc gacctgatcg cgctctttgc
      721 cggacttctc gccctcttct cgcacaggga ggggggggca gggtgatgga gagagtcctg
      781 gatgccggcg gcggaccgcg catcgtctcc tggaacgtca cggcccggtg caacctcgcc
      841 tgcgcccact gctacatcga cgcggacggc gagggctccc ccggcgaact cgacaccgcc
      901 gaaggcatgg ccctgatcga ccagatcgcc gccgtcggcc ggccgatcct ggtcctcagc
      961 gggggtgaac cgctcctccg ccccgacatc ttcgaccttg cccggtatgc cgccgggcgc
     1021 gggcttcgcg tcgccatggg cacgaacggc atcctcgtca ccgatgccgt tgcagaacgg
     1081 ttgagcgata ccggcatccg gaaggtggcg gtcagcatcg actccacgga cccgggggtc
     1141 cacgacgcct tcaggggcgt cagtggcgcc tgggagaagg ccgtcaacgg catccgggct
     1201 ctccggaggg cgggggttcc ggtccagatc cacacgacga tcggtgaacc tgacatcggc
     1261 gagatcgaca ggatcatcgc gtttgggaag agtcacggcg tccgcgacta ccagttcttc
     1321 ttccctgtcc cgacgggccg gggagaggac ctcgccggcc tccccccgga ggttcacgag
     1381 gcggttatcg ggcgcatcct cgcccacgcc cgggaaccgg ggttctcggt ccggccgaca
     1441 tgcgccccgc agtttgtccg ggtcgccgcc cggatggggc ttgacctctc gcagtggggc
     1501 aggggatgca tcgccggcac cgcatactgc cggatcaccc cggtcggaga tgtgacgccc
     1561 tgcccatacc tgcccgtccc ggttggaaac atccgaagag aggctttcag cgatatctgg
     1621 cacggctccg agatcttcgc cgccctccgc gaccccgaca gcctcggggg gaagtgcggg
     1681 caatgcgagt accgccgggc ctgcggcgga tgccgtgcac gggcttacgg ggtcaggcac
     1741 cggcgcggcg atgcctgcgg cggcccggtg cgccccggcg acccggacgg cgatatactg
     1801 gcggaagacc cctgctgccc ctacgaaccc ggagagataa cgcatgactg accggcacct
     1861 ggacatcctt gaccgacaac tcctcgagat actgcaggat accttccccc tggcaccgcg
     1921 gccgtggcac gcgatcggga ggcgtcttgg cataccggag ggcgaggtgc tccggcgggt
     1981 gcgccgtctc gccgatgaag gtatcgtcct gaccgtctcg ccggtcattg agtcggcgcg
     2041 gatcggcccc gtggcctcca cgctcatcgg catgcgggtg ccggcggacc gggtggagcg
     2101 gtgcgcatcg atcgtcaacc gggaacccgg tgtctcgcac aactacctcc gtgaccacga
     2161 ctacaacctc tggttcacgc tcgccgcgcc ggacagcgcc gccctgcgcc ggacggtgga
     2221 agatatcgcc gcccgggccg gcgtcccccc ggccgatctc ctcgacctgc cggcggtgcg
     2281 gcggttcaag ataggcgtcc ggttccggtt cttcccggac ggcgacgggg agggcggcat
     2341 atgaacgacc gcaccctcct catcaggctt ctctcgggcg tcccgctggt ggaggagccc
     2401 taccgggcga tcgcggagga actcggcgtg acggaggcgg aagtgatcgc ccggattgag
     2461 tcccttctct cgcagggcat catccggcgg ttcgcggtgc ggcttgacca ccggagagcc
     2521 ggcatccggg tcaacgcgat ggtcgcatgg cgggtgccgc ccggggatat ggtgcaggta
     2581 gcagagatca tggcccggtc cccggaggtg acgcactgct acgaacgagc cactgttccc
     2641 gggcggtggg attacaacct ctacgccgtc ctgcacggcc ggcgtcgcgc cggggtcgac
     2701 cgctccattg cccgcctctc ccgggcggcc ggcatcgatg actgcgcagt cctcgtcagc
     2761 acccgggagc tgaaacgcac acccccgggg ttagcgctcc tcgcaaagga ggggagatga
     2821 gtgatccggt tcacgcacct catacacggg gaagggccgg tcagcggccc ggcggcgaag
     2881 catagccacc ttgcgtttga ggaggggcgc cgcgggccgg tggtcttctg gaacatcaca
     2941 tcgcggtgca acctcgcctg tacgcactgc tacctccggt ccgggccccg ccggcggcgg
     3001 gaggatgaac tgacgacagg cgaggcaaaa gctctcatcg acgaccttgc tgcggcgaac
     3061 gtcccgctcc tcctcttctc cggcggggaa ccgctgatgc gggaagactt ctggagcctc
     3121 gcggcgcacg ctcaagagcg cggcatcccc gcggtcctga gcaccaacgg gacactgatc
     3181 acaccaaccg tcgcccgaag gctccgggat gccgggattg ggtatgccgg tatatccctc
     3241 gacggcgcga cggcggcaac ccacgacctg ttccggggag tggtgggaag tttcgaccgt
     3301 tcggtgcagg cgctgaagta ctgcatcgat gcgggcctcc ggtgcggcgt ccggttcacg
     3361 gtcacgaaga ggaaccacag tgaactcggg cgcctgatcg cgcttgcccg gtcgatcggg
     3421 gtgcaccggt tctgcgtcta ctggctcgtc ccgagcgggc gggggggcga cgcacacgcc
     3481 gacctgcagg tcgggccagg ggaggtgcag ggagtcatcg atctgatcta ccgcgagacc
     3541 ctcaggaccg atccggcggc gatggagttc ctgaccgtcg atgctcccca ggacggcgtc
     3601 tgcctgcttg agatgctgaa agcggatgcg catccggcat acgaccggac ataccgcctc
     3661 cttgcccgca tgggcggatg cagcgccggg aggcgggtgg cgaatatcga cccctccggg
     3721 aacgtctacc cctgccagtt tgcgcaattc cgggaatttc tcgcgggcaa catacgagac
     3781 cggccgttta gcgagatatg gagcgacccc ggcaaccagg tgcttacggc gttccgggag
     3841 ggcgaggcac gccccgggag cccgtgcagc acctgcgacc ggcaggaggt ctgcggtacc
     3901 gggtgccggg tccgggccta cgtccgatac ggcgatctca acagcggcga cccgctctgc
     3961 ctccggcacg ggtgagcccg ggggagggca atatttaaat atcgggtgcg ttatggtctc
     4021 tcccatgagg cctaacgtaa tcctcatcgt agcgctcctt gtcgtgagcg ccggagttct
     4081 ggccgcgggg tgcaccacgc cgggtgagaa tgtcacccag ccgacggctc ccccgaccgg
     4141 gaccgagaca ccagcagtca cagcgacgac cgtcgctgag acaccagcgg ctacagggac
     4201 aaccaccgct gagacaccgg cggctacggc gacaaccgcc gccgagacac cgacgactgc
     4261 agagacgacg gcggcgacca caaccacagc ggcaacaaac gccacaacgg gagcgaccac
     4321 tgcggctggc ggcgggactg cgactgttga cctcaccgcc cagaacatcg ccttcgacaa
     4381 gagcaccatc acggtgccgg cgggggcgga agtaacggtg aacttcgaca accaggactc
     4441 gggcatcccg cacaacttcg cggtctacac cgactcctct gcgagccaga gcatattcaa
     4501 gggcgacacc atcaccggcc cggcaacgac cacctacacc ttcaccgcgc ctgatgaggc
     4561 cggtacttac tacttccgct gcgacgtcca ccccgagcag atgaacgggg acttcgtcgt
     4621 gcagtgatca atccacccac tctttttccc atctccgctt ccggtaaccg ttatgaccgc
     4681 ctgatccgat cgggggccgc cgtgaaaccc ctgctattcc tgatagtatc cccggcagtt
     4741 gccgggatgc tccttcctcg cagccggaag tcccggcgac gaccacctac accttcatcg
     4801 cacccgataa acccggtacc tactgcttcc gctgcggcgt ccaccccgaa gagatgaaag
     4861 acgatctcgt cgtgcagtga tacgcaccac cggcgggaga tcccccgatc tcccccttct
     4921 tcgcccccgg tgtcgcccgg cataacgttt atatggggtg agccccatgc ccgggctcgg
     4981 gggggatgac cacgccggcc gggggagcat catcggcacc tgatgagcat gactcaaagg
     5041 gcgataccag gaagatcatc gggatcctgg ccgcgcttgc gatcttcatc atcgtcctct
     5101 acggttatat catacccctg cagggcgggt tcatatcctc tacggcgata cgggctgatg
     5161 atctcctggg agcggatccc cggttcgagg agcagatgcc gatccaggag gtcgacctcg
     5221 gggcctccgg ccggtcgatc tt
//
LOCUS       DAOSEE010000138         5213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_5641_length_5213_cov_261.881155, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000138 DAOSEE010000000
VERSION     DAOSEE010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5641_length_5213_cov_261.881155"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="PLI76_09380"
     CDS             complement(<1..352)
                     /locus_tag="PLI76_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62286.1"
                     /translation="MPGKKEVTIGITVNLENYENLRLEVEGDVETHEDVDDLITFLDG
                     MLARLGRGDPATAERVDAYRRRVLKTRPAEPQAPAARPVPEREPAGTAAVEPAHRQEE
                     AISPPVGPIEAAIPP"
     gene            801..2333
                     /locus_tag="PLI76_09385"
     CDS             801..2333
                     /locus_tag="PLI76_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOI62287.1"
                     /translation="MTTRLPERPGTLPGPCQPIALLIGAVICLLLAQSVAAAPLFVAA
                     SDSSPADRAQADYTCDGVDDQVEIRAALAALPGGGTVALANGTFNCSGSLIPRANTTL
                     RGEGPDATFILFRENGRLNVSEEHITLDGFQIAGADYKNMSTDTTLDLGRWLGVLTIY
                     ASHTKVRNVTGTADASIQAVFLLLHNPNVYASVLEDVEFTNCRAVDTGTYGFLHNAWG
                     SENTTIRNIRYENCSAINCGRYGAFNAWVTGFDFAEQNNIEGLRVSNCSARGSLESGF
                     HFEWDAEKRDCVFINCVSTENGQKPWPETYLVGDPHYFGAGFYLPGGRVSLVNCHAGD
                     NRAFGYFIGNPDGMLLYNCTENGTGRSPIFGWSARPIAYCILQSLPVTKNPSVVMDHC
                     SSLDTNGWGLFICGASNVLIRNFTLTDPAGIRGVGAMLGCPGDELPVNSTIGVGISHS
                     AIDLAASGSRPHTLIYIVNNRNVTYSGRIASGAAHPVLFEGSAGDVDLSALKVLPASG
                     TA"
     gene            2691..4340
                     /locus_tag="PLI76_09390"
     CDS             2691..4340
                     /locus_tag="PLI76_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62288.1"
                     /translation="MSAKPYRGARNRRIRIAIALLIALAGIAVLVLHLYPPVPAPPPP
                     PPPPFTPLPTTPVVPPEVPGSTITVAASDSSPASKARADIQCDGIDDQRDIQAAFDAL
                     PSGGTVALTEGRFNCAGSIYPHANTMLRGEGPDKTFVEFSRNGRLIVSRESVTLYNFH
                     VEGTNYPAGDDRWLGVVTIYASHARILAVEGTADATTQAVFLLLHDPGVYVPVLQDIE
                     FTNCRAADTGTYGFLHNAWGTENRVIRDIRYENCAAINCGRYGAFNPWVTGFDFAELN
                     DIEGLRVKNCLAEGNLESGFHFEWDPEKRDCILENCVSRNNGQKSYPTKPYQEDDTST
                     HYFGCGYYAPRGDITFVSCYSEENSQYGFYATNGGKLYACVDRGVGAGKTDYRIIQPA
                     SFYAAPTRSIAPSLILENCSSIDSNGYGLHIDFASDVRIVSFHLENPAGIDGKATNLG
                     GSQGGPLTNSVVNIYASGDRAETLIWARNNENVEYSGRIISNTAKPFVIEGDRTRKVR
                     VRDMEIVSANLAPFTNGVVLASTVPAGAVTFENVAVIPAVR"
     gene            4882..>5213
                     /locus_tag="PLI76_09395"
     CDS             4882..>5213
                     /locus_tag="PLI76_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOI62289.1"
                     /translation="MFLIVGLVPIVGALETTPTTIVAASDSSAAAKAAAAYVCDGVND
                     HVEIQAALNALPSSGGVVLLSSGTYNCAGIIAPKAGSTLKGEGETKTNLVFTHDGRIN
                     VDREYVTL"
BASE COUNT         1012 a         1612 c         1568 g         1021 t
ORIGIN      
        1 ccggcggaat tgccgcctcg atggggccga caggaggaga gattgcctct tcctgccggt
       61 gtgcaggttc aactgccgcc gtcccggcag gctctctctc cggcaccggt cgcgcagccg
      121 gtgcctgcgg ctctgcaggg cgggttttca ggacgcgccg gcggtatgcg tcgacccgtt
      181 cggcggtcgc aggatccccg cgcccgagcc gggcaagcat cccgtcgagg aacgtgataa
      241 ggtcgtctac atcctcatgg gtctctacat cgccctcgac ctcgaggcgg aggttttcgt
      301 agttctcgag gttgacggtg atcccgatgg tgacttcctt ctttccgggc atatgcttat
      361 aattattgtt ttccatatat acaattccac cggccgccgg aattgccctg aaccgcccct
      421 tcttcccgtc atccgcaccc ggcagcgacc cgcaccgtgg atggacgagc cggagaaata
      481 caagcaactt tcctgaaatc ggggcctgat cctgtcctgc cagccctgcg cgcgggctct
      541 cccgccgtga gtatctcaaa cccggatcct ggagcgatgc atcgatttcc tcacccatca
      601 ccttttatac tgccgcggct atacctgccg tgcccgatcg cgccgggccg cccggttctg
      661 gtaaggcggt ccgcctgtcg ggtccggcaa ccggatctcc ggtagatccg gtatgacagg
      721 gcctgctcgc tccatagcag ggcgctaaag cctctggtac agcataccag ggatcctgca
      781 cgatacgacc acggagtgtc atgacgaccc gcctgcctga aagaccggga acccttcccg
      841 gcccgtgcca gccgatcgcc ctccttatcg gcgcggtgat ctgccttctg ctcgcccaga
      901 gcgttgctgc ggcgccgctc tttgtggcgg cgagcgacag cagcccggca gacagggcac
      961 aggcggatta cacctgcgac ggggtagacg accaggtgga gatccgggca gcacttgctg
     1021 ctctgccggg cggaggcacg gttgccctcg caaatgggac attcaactgt tccgggagcc
     1081 tcataccccg ggcgaacacg acgcttcgcg gggaggggcc ggatgcgacg ttcattctgt
     1141 tccgcgagaa cgggaggctc aatgtctcgg aagagcatat caccctggat gggtttcaga
     1201 tcgcaggggc cgactacaag aatatgagca cggatacgac gctcgacctc ggccggtggc
     1261 tcggcgtcct caccatctat gcgagccata caaaggttcg aaacgtcacg ggcactgccg
     1321 atgcgagcat ccaggcggtc ttcctcctgc tccacaaccc caacgtctac gcatccgtcc
     1381 ttgaagacgt cgagttcacg aactgccgtg ccgtggacac cgggacctac gggttcctcc
     1441 acaacgcctg gggatcggag aacacaacga tccggaacat ccgctacgag aactgctccg
     1501 ccatcaactg tggcaggtac ggggcgttca acgcctgggt gaccgggttt gactttgccg
     1561 agcagaacaa catcgagggc ctcagggtga gcaactgctc cgccaggggg agccttgagt
     1621 ccgggtttca cttcgagtgg gacgcggaga agcgggattg tgtcttcatc aactgcgtga
     1681 gcacggagaa cgggcagaaa ccctggcccg agacctacct ggtgggcgac ccgcactact
     1741 tcggggccgg cttctacctc cccggcggac gggtatccct ggtgaactgc catgcagggg
     1801 ataacagggc gtttggttac tttataggca atccggacgg gatgctcctc tacaactgca
     1861 ccgagaacgg caccggacgc tcccctatct tcggctggtc tgcgcgacct atcgcctact
     1921 gcatactcca gtcgctgccg gtcacgaaga atccatccgt cgtgatggac cactgcagca
     1981 gcctcgacac gaacggatgg ggccttttta tctgcggcgc atcgaacgtg ctcatcagaa
     2041 acttcaccct gaccgatccg gctggcatca ggggggtcgg ggcgatgctg ggatgtcccg
     2101 gggatgaact cccggtgaac tcgacgatcg gggtgggcat ctctcactcg gccatcgatc
     2161 ttgccgcatc gggaagccgg ccccatacgc tgatatacat cgtgaacaac agaaacgtca
     2221 cgtactccgg caggattgcc tccggcgccg ctcatccggt cctgttcgag gggtcggcgg
     2281 gggatgtgga cctgtcggcc ctcaaggtat tgccggccag cggtacggcg tagcggcggc
     2341 atcgccgggg ccgggagtac cccggatccg tttgaaaaaa acttcacata tgccgcttcc
     2401 ccccctgccc ggcaaggata ccccgacatc agccctgcgg aaccctgggc ccgaccctcc
     2461 gggggaacgg ggcgactaaa ctcttaaatg gtacctccgc gctatacggg gtgttgaccg
     2521 ggcaggaggc agacgggtca tccgtacccg cgcccgggga gtttccgctg gcagggccgc
     2581 cttcggatgg tccgatccag cctgagcagg gagtgcatcg tcaggtcgcc aggggcgatg
     2641 ccgcaggaga tccgacagcg tgagaagagt aacgggcagg ttgtagtctc atgtcagcga
     2701 agccgtatcg gggggccagg aacagacgta tacgtatcgc catcgcactc ctgattgctc
     2761 tggccggtat cgccgtactg gtcctgcacc tttatcctcc ggtgccggcg cccccgcctc
     2821 ctcctccccc gccgttcacc ccgctgccca cgacaccggt cgtgcctccg gaggtgcccg
     2881 gatcgacgat caccgtcgcg gcgagcgaca gcagccctgc atcgaaagcc cgggccgaca
     2941 tccagtgcga cggcatcgat gaccagcgcg acatccaggc tgcgttcgac gctctgccgt
     3001 cgggaggcac ggtcgccctg actgagggcc ggttcaactg tgccgggagc atctaccccc
     3061 atgcaaacac catgctccgc ggggaggggc cagataagac gttcgttgag ttcagccgga
     3121 acggtcggct catcgtctcc cgtgaatccg tcacgctcta caactttcat gtcgagggca
     3181 ccaactatcc cgccggagac gatcgctggc tcggtgtggt caccatctac gcgagccacg
     3241 cgaggatcct tgccgtcgag gggactgccg atgcgaccac ccaggcggtc ttcctcctcc
     3301 tccacgaccc gggtgtctac gtgccagtgc tccaggatat cgagttcacg aactgccgtg
     3361 ccgcggacac cgggacctac gggtttctcc acaacgcctg gggaacggag aacagggtga
     3421 tcagagatat ccgttacgag aattgcgccg caatcaactg cggcaggtat ggggcgttca
     3481 acccctgggt gaccgggttt gacttcgcgg agttaaacga catcgagggg ctccgcgtga
     3541 agaactgtct cgcggaggga aacctggagt ccggctttca cttcgagtgg gaccccgaga
     3601 agcgggactg catacttgag aactgcgtga gcagaaacaa cggacagaaa tcctacccga
     3661 cgaagcccta ccaggaggac gatacgtcca cgcactactt cgggtgcggc tactacgctc
     3721 cccgggggga tatcaccttc gtcagctgct actccgagga gaacagccag tacgggttct
     3781 atgcgacgaa cggcggcaag ctctacgcct gcgtcgaccg gggcgtcggc gccgggaaga
     3841 ccgattaccg tatcatccag ccggcatcgt tctacgccgc ccccacccga tcgatagcgc
     3901 cgtcgttgat actggagaac tgctcaagca tcgactcaaa cggctacggc ctccatatcg
     3961 actttgcaag cgacgtccgg attgtgagct ttcacctgga gaacccggcc ggaatcgatg
     4021 gaaaggcgac gaaccttggc gggtcgcagg gagggccgct cacgaactcg gtggtgaata
     4081 tctatgcgtc gggtgaccgg gccgagacgc tgatctgggc ccggaataac gaaaacgtcg
     4141 agtattctgg caggatcatc tcgaacaccg cgaaaccgtt cgtgattgaa ggagatcgca
     4201 ccagaaaggt ccgggtcagg gatatggaga tcgtctccgc gaacctcgcg cctttcacca
     4261 acggtgtggt cctcgccagt acggtgcctg ccggagcggt gacgttcgag aacgtggctg
     4321 ttattccggc ggtgcggtga cgcaccgatc gccattccgg gcctggcccc gggagccccg
     4381 atgcattgca cgggttctac cggcgaccgg gtagtcgaga ttgtttgccg gccgatcccg
     4441 ccctgtcccg ggcattgcat ggggggtgcc gcgggtattc ccctccggtc ctccggggtg
     4501 cggccggggg cggcagatcg cccccgcctg ccggatccgg ggatctatga cttttccggg
     4561 tttttcatga atttttcagt aatccggggc ttatttgcct gccagaacta cgcccctcgt
     4621 tcccgggaca gaacgggttt tatcccgggt gcacgcgcta aagtccaatt tcatatatat
     4681 ctatttctat aatagtacaa ctttaattat aattggaatt cattatttca aaagagtata
     4741 atcctctttt ttggcttctt agatgattat atcaaataaa tcaaaataag aaacttctta
     4801 agttaggttt ttcagataat gcagtatctc ccgagaggtg agataatgcg agatagacgg
     4861 acaacaaagc ttttaatcgc ggtgttcctg atcgtcgggc ttgtcccgat agtcggggca
     4921 ctcgagacga cccccaccac gattgtcgcg gcaagcgaca gcagtgcggc cgcgaaagcg
     4981 gcggctgcct atgtctgtga tggggtcaat gatcatgtgg agattcaggc agcactgaat
     5041 gccctgccat catccggtgg tgttgtcctc ctttccagcg gcacatacaa ctgtgctggt
     5101 atcattgctc ccaaagcagg ctctaccctc aagggggagg gtgagacgaa gacgaacctt
     5161 gtcttcaccc atgacggtcg catcaacgtc gaccgtgagt atgtgaccct gga
//
LOCUS       DAOSEE010000139         5153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_5728_length_5153_cov_215.124166, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000139 DAOSEE010000000
VERSION     DAOSEE010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5728_length_5153_cov_215.124166"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..1212
                     /locus_tag="PLI76_09400"
     CDS             103..1212
                     /locus_tag="PLI76_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04013 family B12-binding domain/radical SAM
                     domain-containing protein"
                     /protein_id="HOI62290.1"
                     /translation="MKIYWRHIPQANNSYAALSAACEVHGYNLEAAEGPRPDVTCYSI
                     NSIDERLYRDEIAGADCITVVGGPHASACYREVARYADYVVVGEGEYTLPALLFAIEE
                     GRDPPPGVATAAGYTPARHTVLLDGHPPFSAVKGFIEITRGCPFSCGYCQTPRLFGRC
                     MRHRSVDEISRYAARFRDIRFVTPNALAYGSDGVHIRLDRVERLLRSLDGRVYFGTFP
                     GEVRPECISQQALDLIVETCANTRLHFGAQSGSDRVLRLLHRGHTVEDVVRAVDLCRD
                     NGLVPVVDFIFGLPFESDDDQRATLDLVRMVTRAGRAHIHYFMPLPGTPLKQSRPRPL
                     LPETEKILGKLALDGRITGSWMDHEIRFFRRTPHL"
     gene            1216..1857
                     /locus_tag="PLI76_09405"
     CDS             1216..1857
                     /locus_tag="PLI76_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOI62291.1"
                     /translation="MTDVLLLADLHGNYGKLDAFLSYDYDAVIIAGDITNFGPLEPVD
                     SVLSRFEVPCFAIPGNCDPREIIDVLERSDTICLHNSWIALGKITLAGFGGSNTTPFG
                     TPFELTEEEIDSELTRIVNHMDRNIHNVLLSHAPPYGVLDSVGENHVGSRSIRKHMTR
                     FDLICCAHIHEARGIVEVDGVRVVNPGPASEGYGALIHFGKEPKDIDIELISV"
     gene            complement(1844..2374)
                     /gene="cyaB"
                     /locus_tag="PLI76_09410"
     CDS             complement(1844..2374)
                     /gene="cyaB"
                     /locus_tag="PLI76_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HOI62292.1"
                     /translation="MLEVEAKFAVRDPGEVRARLDRQGVHMARRQQEHDIYYNAPHRD
                     FAKTDEALRVRYDDAGVTVTYKGPKIRVGSAKAREEFNLAVASGETLEAILSRLGFRR
                     AASVSKTREFYEMGDVTVTLDDVEGLGTFIEIEILTESDREDAARRIGVTAKGLGVDG
                     PPIYTSYLEMLVSKQK"
     gene            complement(2417..3868)
                     /locus_tag="PLI76_09415"
     CDS             complement(2417..3868)
                     /locus_tag="PLI76_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOI62293.1"
                     /translation="MDTVQQQAEAVSYAPAPPEEFCGREEERGRLSAIISRAGEQGQA
                     VMISGPSGIGKSSLLSWLAHEVRGRPGGLPVPVLRAEVFDLPGMIFPAFRELVNDLRR
                     YAAPGREGLREAIRYAGDVLEKYAAPVEPVGLLPKTGEEIVGIFARSPDVGYDRVCRA
                     FGELLREIGREMAGTGRIAAILLDDVHLASHLDRRLLRDTVRDLPPGILLAFTCRRED
                     GSGYIAICEELRDSRVQEVQLAGMGRNEIRELGEKRFDLAIDDAAAGILEETRNDPFG
                     LVACFNALRRQGLAPSAGNIAGVLAGAGEPVALAFATLTGPERVLVEELCILNPPFPA
                     PVVACMLRFTEADMIRGSGVLRKLPGAGYAFVHPLLQEYCRRELPDDARITLNARAAD
                     CFERTMHRLPGRLHALLSLACHSFYAREYGKAADLNLELGLRFSHREDYDTALVLTER
                     AIASAEQIGDDLLLAAARRQDGLIREKIAGSTG"
     gene            4106..4867
                     /locus_tag="PLI76_09420"
     CDS             4106..4867
                     /locus_tag="PLI76_09420"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOI62294.1"
                     /translation="MALQEGDFIRLSYTGRAGENIFDTTDEENAKEAGIHNPQAEYGP
                     VTIRLGSHHVIIGLEEALIGKEVGAEGEVTVPPEKAFGAHEEKYVRSVPTTQFHGKPK
                     RGMSVEVEGREGIITDIIGRRAVVDFNHPLAGKTLSYSYAILDMVDDRVEQIKGLIKL
                     YAGRTDIGISIVDGTVELALPAAIMYDRRWMAWRGTLIRGIFEYYPDITNVVMKETFP
                     RPEEPGEEPEAPAAEDAAEETEAPAVEGAAEEAGE"
BASE COUNT          961 a         1676 c         1599 g          917 t
ORIGIN      
        1 atgggtcgtc accttaattg catgcgcggc gagagtacag gatacgtatg ccgtgcaggg
       61 aaacccggca tgtgcatgct ccacgactgg aacacagcag ccatgaagat ctactggcgc
      121 cacatccccc aggcaaacaa ctcctacgcc gcgctctctg cagcgtgcga ggtgcacgga
      181 tacaatctcg aggcggcgga gggccctcgc cccgacgtca cctgctacag cataaactcc
      241 atcgacgaac gcctctaccg cgacgagata gcgggggcgg actgcataac cgtcgtcggg
      301 gggccccacg cctccgcctg ctaccgcgag gtggcgcggt atgccgacta cgtcgtcgtc
      361 ggggagggcg agtacaccct cccggcgctt ctctttgcca tcgaggaggg gcgggacccg
      421 cctcccggcg tcgcgaccgc cgccggctac acgccggccc gccataccgt cctcctcgac
      481 ggccatcctc ccttctcggc ggtgaaaggg ttcatcgaga tcacccgggg atgccccttc
      541 tcctgcggct actgccagac gccccgcctc ttcgggcggt gcatgcgaca ccgttccgtc
      601 gacgagatca gccggtatgc cgcacggttc cgcgacatcc ggttcgtcac cccaaacgcg
      661 ctcgcttacg ggtcggacgg ggtccacatc cgtctcgacc gggtggaacg gctcctccgg
      721 agcctcgacg gccgggtcta cttcggcacc ttcccgggcg aggtgcgccc cgagtgcata
      781 tcgcaacagg ccctcgacct catcgtcgag acctgcgcga acacgcgcct gcactttggc
      841 gcccagtcgg ggagcgaccg tgtgctccgt ctcctgcacc gggggcatac cgtggaggac
      901 gtcgtccgcg ccgtcgatct ctgccgggat aacggactcg tcccggtcgt cgatttcata
      961 ttcgggctgc cgttcgagag cgacgacgac cagcgcgcaa ccctcgatct ggtgcggatg
     1021 gtcacccggg cggggagagc gcacatccac tatttcatgc cgctgcccgg aaccccgctc
     1081 aagcagagcc ggccgcgccc gctcctcccg gaaacggaga agattcttgg caaactggcg
     1141 ctcgacggca ggataaccgg ctcatggatg gaccatgaga taaggttttt tagacgcact
     1201 cctcatctat agtgcatgac ggatgtgcta ctcttagcag atctgcacgg taactacgga
     1261 aagcttgacg cttttctcag ctacgactac gatgcagtca tcattgcagg cgacatcacc
     1321 aacttcggtc cacttgagcc tgtagattcg gtactctccc gtttcgaagt accttgtttt
     1381 gcaattcccg gtaactgcga cccccgtgag atcatcgacg tgctcgaacg ctcagatacg
     1441 atctgtctac acaactcctg gatcgcgctt ggcaagataa cgcttgcggg ttttgggggt
     1501 tcgaacacga cccccttcgg cacgccgttc gagctgaccg aggaagagat cgacagcgaa
     1561 ctcacccgga tcgtcaacca catggacagg aacatccaca atgtcctgct cagccatgcc
     1621 ccgccgtacg gagtgctgga ctctgtcggt gaaaatcacg tggggagccg gagcatcagg
     1681 aaacacatga cccggttcga cctgatctgc tgtgcccata tccacgaggc acggggtatc
     1741 gtcgaggtcg acggcgtcag ggtcgtcaac cccggacccg cctcggaggg ctacggggct
     1801 ctcatccact tcggaaagga gccgaaagat attgatatcg aacttatttc tgtttagata
     1861 ccagcatctc gagatatgag gtgtagatcg gtggaccgtc tacacccaac ccttttgcag
     1921 taaccccgat ccgccgggcg gcgtcttccc gatcgctctc cgtcaggatc tcgatctcga
     1981 tgaacgtccc gagaccttcg acgtcgtcga gggtcaccgt cacgtccccc atctcgtaga
     2041 actcccgggt ctttgagacg gatgcggccc gccggaaccc gaggcgggag aggatagcct
     2101 caagggtctc gcccgaggcg accgcgaggt tgaactcctc gcgggccttt gcgctcccga
     2161 cccggatctt cggtcccttg taggtcacgg tgaccccggc atcgtcgtac ctgaccctca
     2221 acgcctcgtc ggtctttgca aaatcacggt gaggggcgtt gtagtagatg tcgtgctcct
     2281 gctgccttct tgccatatgc actccctgcc ggtcgagccg ggccctgact tctcccgggt
     2341 ctctgaccgc aaactttgcc tctacttcga gcataccggt cgcctcagat cgtctcttct
     2401 cgatggcgat aaaccctcac cctgtggagc cggcgatctt ctcccggatg aggccgtcct
     2461 gcctgcgtgc ggcggcgagg aggagatcgt ccccgatctg ctctgcggag gcgatcgccc
     2521 gttcggtcag cacgagcgcc gtatcgtagt cttcacggtg ggagaaccgg agaccgagct
     2581 ccaggttcag gtccgccgcc ttcccgtact cccgcgcata gaaggagtgg caggcaagcg
     2641 agaggagggc gtgcagcctg cccgggaggc ggtgcatggt gcgctcgaaa cagtccgccg
     2701 cacgggcgtt gagcgttatc cgcgcatcgt ccgggagctc gcgcctgcag tattcctgca
     2761 ggagtggatg gacgaaggca tacccggcac ccgggagctt ccgaagtacg ccgctccccc
     2821 ggatcatatc cgcctccgtg aacctcagca tgcaggcgac gaccggcgcc gggaaaggag
     2881 ggtttaagat gcagagttcc tctaccagga ctctctccgg gccggtgagc gtggcaaagg
     2941 cgagcgctac cggctccccg gccccggcga gcacgccggc tatgtttccg gcggagggcg
     3001 cgagaccctg ccggcgcagg gcgttgaaac aggcgacgag gccaaacggg tcgttacggg
     3061 tctcttcgag gatgccggcg gccgcatcgt cgatagcgag atcgaaccgc ttttccccga
     3121 gctcccggat ctcgtttctc cccatgccgg caagctggac ctcctgcacc ctggaatccc
     3181 ggagttcctc gcagatcgct atgtacccgg atccgtcctc cctccggcag gtgaaagcaa
     3241 ggaggatgcc gggcgggagg tcgcgtacgg tgtcccggag gagccggcgg tcaaggtgtg
     3301 atgcgaggtg gacgtcgtcg agcaggatcg ccgcgatgcg gcccgtcccg gccatctctc
     3361 tcccgatttc ccggaggagt tctccgaacg cccggcacac ccggtcgtac ccgacatccg
     3421 gcgaccgggc gaatatgccg acgatctcct ccccggtctt cgggagcagg ccgaccggct
     3481 ccaccggggc ggcatacttc tcgagcacgt cgccggcata ccggatcgcc tcccgcaggc
     3541 cttctctccc gggggcagcg tacctgcgca ggtcgttcac cagttcccgg aacgccggga
     3601 agatcatccc gggaaggtcg aagacctccg ccctgaggac gggcaccggc agcccgccgg
     3661 ggcgcccccg gacctcatgg gcaagccagg agagaaggga gctctttccg atgccggatg
     3721 ggccggatat catcaccgcc tgcccctgct cccccgcccg ggatatgatg gccgagagcc
     3781 gtccgcgctc ctcctccctg ccgcagaact cctccggggg agcgggagca tacgagaccg
     3841 cctccgcctg ctgctgcacc gtatccatga tcccggccac gattgagccg ggtatacttg
     3901 aacgtttggg cggaggcggc cccacctgaa gcatgaatga agcgcgtttt tcctgcagtc
     3961 gccgccctcc gggaggagcg cctgcccgcc gggcagcccg gtagacacta taaataccat
     4021 tccatccaaa tggattagag caacgagaaa caggcgtgcg ggagatcggt aaccggaccg
     4081 cggtctgtaa tttcaggtga atggtatggc acttcaggaa ggagatttta tcagactcag
     4141 ctacaccggc cgtgccggcg agaatatatt cgataccaca gatgaggaga acgcaaagga
     4201 agcgggcatc cacaacccgc aggcggagta cggcccggtc accatccgcc tggggagcca
     4261 ccacgtcatc atcggccttg aggaagcgct gataggcaaa gaggtcggcg ccgagggaga
     4321 ggtcactgtc ccgcccgaga aggcgttcgg ggctcacgag gagaagtacg tgcggtccgt
     4381 cccgaccacg cagttccacg gcaaaccaaa gcgcgggatg tcggtcgagg tcgagggccg
     4441 cgaagggatc atcaccgata ttatagggcg gcgtgcggtc gtcgacttca accacccgct
     4501 tgccgggaag accctgagtt actcctacgc gatcctcgat atggtcgatg accgggtaga
     4561 acagatcaaa ggcctgatca agctctacgc gggccggacc gacatcggta tcagcatcgt
     4621 cgacgggacg gtcgagctcg cacttcctgc ggcgatcatg tacgaccggc gctggatggc
     4681 ctggcggggc accctcatcc gcgggatatt cgaatactac cccgacataa cgaacgtcgt
     4741 catgaaagag acgttcccgc gcccggagga accaggggaa gagcccgagg cccctgcggc
     4801 tgaggatgca gcggaagaga ccgaggcccc tgcggttgag ggagcagcgg aagaggccgg
     4861 ggagtaatcc tcccggttct tgcttttttc aaaggatatc tcccgggccg cgaatcgtgg
     4921 ggatcctcga actcccggag aggccgggtg catgaggcct gccggccgtc gttatcgtat
     4981 cccgcccgct atccttgcgg cgatgctcga ttaccggcac gttcgaagag ggaggtcgta
     5041 atgccggggt acctgcaccc ccgggttccc tgccggctat gtacgggcga ggtagggatg
     5101 ccggcgaggt atacggatcc cctgccaaag gctggggtgg gatcgacggg aag
//
LOCUS       DAOSEE010000140         5150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_5730_length_5150_cov_251.914033, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000140 DAOSEE010000000
VERSION     DAOSEE010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5730_length_5150_cov_251.914033"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            305..1114
                     /gene="minD"
                     /locus_tag="PLI76_09425"
     CDS             305..1114
                     /gene="minD"
                     /locus_tag="PLI76_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATPase MinD"
                     /protein_id="HOI62295.1"
                     /translation="MIRAYTIASGKGGTGKTTVVANLGTALAQYGRETCIVDTDIGMA
                     NLGLVLGLTRTPVTLHEVLAGKAPIQDAIYEGPYGLKIVPSGPSLEGFQNADPARLKD
                     VMTDLTDRCDYLFFDAPAGISTDAIMPLSVADEVLLVVNPELSSLVDALKIRILTEMV
                     GGTVMGTLLNRMTMESTDVSRGKIEKALGVPVIDIIPEDANVRRAAAARMPVVAKYPQ
                     SESSKAFRRIAATLAGLPIEEEPEKVREGFIERFARALFRRDSECSTSMDS"
     gene            1090..1290
                     /locus_tag="PLI76_09430"
     CDS             1090..1290
                     /locus_tag="PLI76_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62296.1"
                     /translation="MFDINGLMIVLLSVIIICLLFIMYVMYVRIQQLLTEVDALTGRM
                     EITGSELEQLTRNVEEYKRMRG"
     gene            1355..1428
                     /locus_tag="PLI76_09435"
     tRNA            1355..1428
                     /locus_tag="PLI76_09435"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1390..1392,aa:Pro,seq:ggg)
     gene            1470..2327
                     /locus_tag="PLI76_09440"
     CDS             1470..2327
                     /locus_tag="PLI76_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HOI62297.1"
                     /translation="MRLHQGCTDCLISRVEYESRLCTDDPARIRAIVEACRELLARSL
                     ADPVPAPVIASRVHRLAYRMLGSTDPYRALKQANNGDALAVCRRVRGSLATFHDLVLA
                     SVIGNTLDYGSKAHTVTDDFVGFFHREFAFGLTVDDTDRMEPLTARVVYLADNCGEIV
                     FDALLAEHLRKSGSHVTFAVRGAPILNDATIEDALALGLDRRVDILTTTTDGIAELGL
                     NRDLLPPILADALDRATLVIAKGMANYESLSDEGDLPPVAYLMSVKCGPIGADIGIPV
                     GSRVALLRE"
     gene            complement(2852..4066)
                     /locus_tag="PLI76_09445"
     CDS             complement(2852..4066)
                     /locus_tag="PLI76_09445"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOI62298.1"
                     /translation="MDIGTLADAGKLKGTVLLRVDLNSPIDPSSNQILDDKRFREHLP
                     TVQALDDARLVILTHQSRPGKKDYTTLEAHAAKFERLLGRPVTYVDDVFGRCARDAIR
                     GAKRGDILMLENLRFAAEENLTLKPEEAKKTILVRRLAAMADFYVNDAFGTAHRSQPS
                     IVGLPLLLPSVAGLLMEKEVANLSRVFTGAPRPVTFVLGGTKVDDSIAVAENVLLRGT
                     ADRVIVVGVVGNVFLLAAGYDIGRPSARLIDDLGYRGEIERARDLLKTYRDRILMPHS
                     VAVREDGGRAEYPVDAVPEDAQVLDIGSESIDRLTEAVSASGTVVVNGPAGLFEEEQF
                     AVGTFDLLRAASTVEFSVVGGGHSGAAIERLGIEDRFTHISTGGGAAIEFLTGKKMPA
                     LEALEMSKKIFG"
     gene            complement(4088..4714)
                     /locus_tag="PLI76_09450"
     CDS             complement(4088..4714)
                     /locus_tag="PLI76_09450"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOI62299.1"
                     /translation="MALRDIKPTRSELINIKRRIKLSERGYNILKMKRDGLILEFFKV
                     LQQAKDSRGALTERYTHAMEMIALAETVEGAIGVKAAAFSASDIPAITLASKNIMGVV
                     VPEIEASSVRKGILDRGYGMLGTSAVIDETAEAFEDLLEAVIEAAEIETTMKRLLDEI
                     ESTKRRVNALEFKVIPELTEARDFIKMRLDEMEREELFRLKKIKARSA"
     gene            complement(4721..>5150)
                     /locus_tag="PLI76_09455"
     CDS             complement(4721..>5150)
                     /locus_tag="PLI76_09455"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843143.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the B subunit is part of the
                     catalytic core of the ATP synthase complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOI62300.1"
                     /translation="ITEGQIVVNRDLHRKGIYPPINVLPSLSRLMNLGIGKGHTREDH
                     KKVSDQLYAAYAEGNDLRGLVAIVGKDALSERDRMFLEFADLFEDRFVRQGLYEDRSI
                     VETLDLGWDLLATLPEEQLVRIDRELIQKYHPKYRKKAQG"
BASE COUNT         1016 a         1614 c         1531 g          989 t
ORIGIN      
        1 ggcactccac ggtgcacgac tttgccgatc cccgccttta tgcgccggtg ctggcaaccg
       61 agcgtgaacg acagggtacc atgcgaaggt cacggcgccg tagcgatccg taagaccccg
      121 ggattgaggc gatccggtat gtccgtaggg caggaacatg aatccgctcc ccggcgccga
      181 tcaaccgggg gcctcacgag ggagagaata cctggtataa aagcagactc tcctttttcc
      241 acgaagagac cgaacgaata agtttaaatt agatcactgc aacctatccc tgagacgatt
      301 ttagatgatc agggcctaca caatcgcctc cggaaaggga gggaccggaa agacgaccgt
      361 ggtcgcgaat ctcggaacag cactggccca gtacggcagg gagacctgta tcgtcgatac
      421 ggatataggt atggccaacc tcgggctggt gctcggactc accaggacgc cggtcacgct
      481 ccatgaggtt cttgcaggga aagcaccgat ccaggatgcc atttatgagg ggccctacgg
      541 cctcaagatc gtcccgagcg gcccttcact tgagggtttc cagaacgcgg atcctgcgcg
      601 gctcaaggac gtgatgaccg atctgaccga ccgctgcgac tacctcttct tcgacgcacc
      661 tgccggcatc agcaccgatg cgatcatgcc cctcagcgtg gcggacgagg tgctgctggt
      721 ggtgaatccc gaactctcct cgctcgtgga tgccttgaag atcaggatcc tgacggagat
      781 ggtcggcggc accgtcatgg ggacgctcct caaccggatg accatggaga gcaccgatgt
      841 gagtcggggg aagatcgaga aggccctggg cgtgccggtc atcgatatca tacccgagga
      901 cgcgaacgtg agaagggcag cggcggcgag gatgccggtc gtcgcgaagt acccgcagtc
      961 cgagtcatca aaagcattcc ggaggattgc ggccacgctg gcgggtctcc ccatcgagga
     1021 agagcccgaa aaggtcaggg aaggatttat cgagcggttt gcccgcgcac tattcaggag
     1081 ggattcagaa tgttcgacat caatggactc atgatagttt tactctcggt gatcatcatc
     1141 tgcctgttgt ttatcatgta cgtcatgtac gtgcgcatcc agcagctcct cacggaggtc
     1201 gatgcgctga cgggcaggat ggagatcacc ggcagcgagc tggaacagct gacgcggaac
     1261 gtcgaagagt acaagagaat gcgaggataa cccctccgtc tccgctctcg ggaggcgaaa
     1321 ctatatgtgc aatttgagca catactactg aggaggggcc atagggtagc ctggccatcc
     1381 taggagactg ggggtcttct gacctgcgtt caaatcgcag tggccccatc cgtacctttt
     1441 tgccttcgcc ggacccagtt atcttcacca tgagactcca ccagggctgc acggactgcc
     1501 tgatctcccg tgtcgagtat gagagccggc tctgtaccga cgacccggcg cgcatccggg
     1561 ctatcgtcga ggcatgccgg gaactcctcg cccgctcgct cgccgacccg gtacccgcgc
     1621 cggtcatcgc gagccgcgtg caccgtcttg cctaccggat gctcggcagc accgacccat
     1681 accgcgcatt gaaacaggcg aacaacgggg atgcactggc agtctgccgg agggtgcgcg
     1741 gcagccttgc gacgtttcac gatctcgtgc tcgcgtcggt catcggcaac acgctggact
     1801 acggctcaaa ggcccatacc gtcaccgacg acttcgtcgg gttctttcac cgggagttcg
     1861 ctttcgggtt gaccgtcgat gataccgacc ggatggagcc cctcaccgcc cgggtggtct
     1921 acctcgccga caactgcggg gagatcgtct ttgacgccct gcttgccgaa cacctcagga
     1981 agagcggatc acacgtcacg tttgccgtca gaggcgcgcc gatcttaaac gacgcgacga
     2041 tcgaggatgc gctggcgctc gggctcgacc gccgggtgga tatcctcacc accaccacgg
     2101 atgggatagc cgagctcggt ctcaaccggg atcttcttcc acccatcctc gccgacgcgc
     2161 tcgaccgcgc gacgcttgtc atcgcgaagg gcatggcaaa ctacgagtcg ctctccgacg
     2221 agggcgatct cccgccggtg gcttacctta tgtcggtgaa gtgcggcccg atcggcgcgg
     2281 atatcggtat ccctgtcggt tcccgggtcg ccctcctccg cgagtgatcc ggcggcagcg
     2341 cgggccgggg ggtaccccgg gcagcgttgc accaatccca aagataacat ataaagaaca
     2401 tggttacaga aaaagattat ttaaacaatt tttatgatta aaagaacggt ttaagacatc
     2461 atttttgcga tttgtcgtca tatatccaca atataattat aggaaaatat tatatagcca
     2521 ataatagaga cattggatcg gggtacgacc acacccagcg accgtcagtc attcaatttc
     2581 catcagcaac gaccacaaac accacacacc tatcagcaat acagggataa caacgaggga
     2641 cgctccgggc acaacacgac gcgcccctgc accagtattc atcggctaca agcgagatct
     2701 caccgggctc acgaagtgtt cgggtgtggg ctccccacct cattttttcg ggaaccagaa
     2761 ccggggagcc acgggtgaca cataacgggg tgtgacggcg ggggtcacct gccgcccgtt
     2821 ccacggtgcc gggcacggac cgcgccctcc ttcacccgaa gatcttcttc gacatctcca
     2881 gcgcctcaag ggcgggcatc ttcttcccgg tcaggaactc gatcgccgcc ccgccgccgg
     2941 tggatatgtg agtgaaccgg tcttcgatgc cgagccgctc gatggccgcg ccggaatgcc
     3001 cgccgcccac caccgagaac tcgacggtcg aggccgccct caggagatcg aacgtgccga
     3061 cggcgaactg ctcttcctca aagagccctg cagggccgtt caccacgacc gtccccgacg
     3121 ccgataccgc ctctgtgagg cggtcgatcg actcgctccc gatgtcgagc acctgggcat
     3181 cctccgggac ggcatcgacc gggtactccg cccgcccgcc gtcctcgcgg accgcaaccg
     3241 aatgaggcat caggatccgg tcccggtagg tcttaaggag atctcgggcg cgctcgatct
     3301 cgccgcggta gccgaggtcg tcgatcaggc gggccgaagg ccggccgata tcgtagccgg
     3361 ccgcgagcaa aaagacgttt ccgaccacgc cgaccacgat gacgcggtcg gccgtcccgc
     3421 gcagaaggac gttctctgcg accgcgatcg agtcgtccac cttcgtcccc ccaaggacga
     3481 aggtgacggg gcgcggggcg ccggtaaaga cccgggagag gtttgcaacc tctttctcca
     3541 tcaggaggcc ggccaccgac ggaagcagaa gcggcagccc cacgatcgag ggctgcgagc
     3601 ggtgcgccgt gccgaacgca tcgttgacgt agaagtcggc catcgcggcg agcctccgta
     3661 cgaggatggt ctttttggcc tcttccggtt tcagggtcag gttctcctcg gcggcaaacc
     3721 ggaggttctc gagcatcagg atatctccgc gcttcgcgcc ccggatagca tcgcgggcgc
     3781 accgcccgaa tacatcgtcc acgtaggtca ccgggcggcc gagcagccgc tcgaacttcg
     3841 ccgcgtgcgc ttcaagcgtc gtgtaatcct tcttgccggg cctgctctgg tgcgtgagga
     3901 tgacaagccg cgcatcatcg agtgcctgca ccgtcggcag gtgctcccga aacctcttgt
     3961 cgtctaatat ctggtttgag gaagggtcga tgggggagtt gagatccacc cgcagcaaca
     4021 ccgtcccctt cagtttccct gcatcagcaa gcgtaccgat atccaccggg tatcacctgt
     4081 accctactca ggcgctccgt gccttaatct ttttcaggcg gaagagctcc tcacgctcca
     4141 tctcatcgag ccgcatcttg atgaagtcgc gcgcctcggt gagctcaggg atcaccttga
     4201 actcgagcgc gttcacgcgc cgcttggtgc tctcgatctc atcgagcagc cgcttcatgg
     4261 tcgtctcgat ctcggccgcc tcgatgaccg cctcgagcag atcctcaaac gcctccgcgg
     4321 tctcgtcgat gacggcggat gtgccgagca tgccgtagcc gcggtcaagg atgcccttcc
     4381 tgacggaaga cgcctcgatc tccgggacga cgacgcccat gatgttcttg cttgcgaggg
     4441 ttatggcggg gatgtcggat gccgagaatg cggcggcctt cacccctatc gccccttcca
     4501 cagtctctgc aagggcgatc atctccatag cgtgcgtata ccgctccgtc agtgcgccgc
     4561 ggctgtcttt ggcctgttgc agcaccttga agaactccag gatcagccca tcgcgcttca
     4621 tctttaagat gttgtagccg cgctccgaga gcttgatccg tcgcttgatg ttgatcagtt
     4681 cagagcgggt cggcttgatg tctcgcagcg ccatggatgc ttacccctgg gccttcttgc
     4741 ggtacttcgg gtgatacttc tggatcagtt cgcggtcgat acgcaccagc tgctcctcgg
     4801 gcagtgtcgc gaggagatcc cagccgagat cgagcgtctc gacgatcgac cggtcctcgt
     4861 agaggccctg cctgacgaac cggtcctcga agaggtcggc gaactcaagg aacatccggt
     4921 cgcgctccga gagcgcatcc ttgccgacga tggcgacaag cccccggagg tcgttgccct
     4981 cggcatacgc cgcatagagc tggtcggaga ccttcttgtg atcctcgcgg gtgtgccctt
     5041 tcccgatacc gaggttcatc aggcgggaga gcgacggcag cacgttgatc ggcgggtaga
     5101 tgcccttccg gtgcagatca cggttgacca cgatctggcc ttcggtgatg
//
LOCUS       DAOSEE010000141         5089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_5837_length_5089_cov_260.790028, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000141 DAOSEE010000000
VERSION     DAOSEE010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5089
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5837_length_5089_cov_260.790028"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..1251)
                     /locus_tag="PLI76_09460"
     CDS             complement(70..1251)
                     /locus_tag="PLI76_09460"
                     /EC_number="4.1.2.43"
                     /EC_number="4.2.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,6,7,8-tetrahydromethanopterin
                     hydro-lyase/3-hexulose-6-phosphate synthase"
                     /protein_id="HOI62301.1"
                     /translation="MYLIGEALVGDGAELAHIDLVMGNKEGAVGQAFANSISQLSRGH
                     TPLLAVIRPNLPTKPSTLVIPKVTLKKEYQVNQMFGPVQAAVAKAVADSVEEGVFEGL
                     DIEDIVIMASVFVHPTAQDYNKIYRFNYGAMKLALRRALDRFPDVETLLHEKDRAAHA
                     VMGFKVQRLWDPPYLQVAMDLVDRNHMRKVLESLPQNDHLIIEAGTPLIKKFGLSIIS
                     EIREIRPNAFIVADLKTLDTGNLEARMTADAGADAVVISGLAPLSTIEKAILDTKKTG
                     IYTVVDMLNVEDPRAVVEQLKVKPDVVELHRGIDVENTAHAWGDIPAIKKAGGERMLV
                     ATAGGIRQHVVKDALHAGADILVVGRAITASKNIKNAAEEFLQELSTEEIDQFRIMTD
                     F"
     gene            complement(1605..1757)
                     /locus_tag="PLI76_09465"
     tRNA            complement(join(1605..1640,1720..1757))
                     /locus_tag="PLI76_09465"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1721..1723),aa:Trp,seq:cca)
     gene            1848..3548
                     /locus_tag="PLI76_09470"
     CDS             1848..3548
                     /locus_tag="PLI76_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOI62302.1"
                     /translation="MVEGSYACGNSQKPLIGITIGEMLNRIAAAHPDSEAIVSVHQGI
                     RWTYAEFLERVNTLARALMALDVERGDRVAIWALNYAEWVLVQFATAKIGAIMVNINP
                     AYRTYEFEYAMKQSEVQTLLLQGRFKTSDYVGMFYESCPEAFEAKPGRINSDKFPFLK
                     NVVFLGDIPYNGMYTWDDLLARAALISPDELREREESLNFDDAVNIQYTSGTTGFPKG
                     VVLTHHNILNNGFIIGEGMKFTHEDRLCIPVPFYHCFGMVLSNMAAVTHGSAMVLPSP
                     VFSAEAVLATVQDERCTALHGVPTMFIAELSHPDFSKYRLDSLRTGIMAGSPCPTEVM
                     REVNQKMNMSEIVIVYGQTETSPGVTMTTTADPLERRVSTVGRPFPHTEIKIVDPHTK
                     RILPRGETGEICARGYCVMRCYYNNPNATRATIDENGWNHTGDLGTMDEEDYVKIVGR
                     LKDMVIRGGENIYPREIEEYLHNHPSVADVYVIGVPDQKYGEELMAWIKLENGANLTE
                     DEVKEFCRGRIAHFKIPRYVKFVDDFPMTVSGKIMKFKMREAAIAELGLECASQIETA
                     "
     gene            3591..4442
                     /locus_tag="PLI76_09475"
     CDS             3591..4442
                     /locus_tag="PLI76_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HOI62303.1"
                     /translation="MHETFGIPEIIEAIQSLLTDPGVNRLGPDVLEPAWESPLIGVSR
                     GDDPLYAEFQETIGPFHWTPLEAFCTEFPDAGVTASDIAVVVWALPQTAATRRDHRAC
                     TALPSRRWSLARHYGEAVNDRIRDHLARTLTNAGYPAVAPARLPVFRRETSERFGIAS
                     RWSERHAAYASGLGTFGLCDGLITERGKAVRLGSVVAAINLPATPRPYSNRTAYCLHF
                     SGDECDACIRRCPAGAISADGHDKERCFAYIREMMVPFVESDIGIPVTSCGLCQTGVP
                     CEGRRPR"
BASE COUNT          950 a         1614 c         1582 g          943 t
ORIGIN      
        1 tattcttcgg gacggcttcg gtctccgcgc cgccgggcaa tttttgccgg aaaaaaaggt
       61 gatgaccgct tagaagtcgg tcattatccg gaactgatcg atctcttcgg tgctcagttc
      121 ctggaggaac tcttcggccg cgttcttgat gttcttgctc gcggtgatcg cccggccgac
      181 gaccaggata tccgcgcccg cgtggagtgc gtccttcacg acgtgctgcc ggatgccgcc
      241 cgccgttgca acgagcatcc gctcgccgcc ggccttcttg atcgccggta tgtcgcccca
      301 ggcgtgggcg gtgttctcga cgtcgatgcc ccggtggagc tccacgacat ccggcttcac
      361 cttgagctgc tcgacgacgg cgcgggggtc ctcgacgttg agcatatcga cgaccgtgta
      421 gatgccggtc tttttggtat cgaggatcgc cttctcgatc gtcgagaggg gggcaagccc
      481 cgatatcacg acggcgtcgg cgccggcatc ggcggtcatc cgggcctcga ggttgcccgt
      541 gtcgagcgtt tttaggtcgg caacgatgaa cgcgttcggc cggatctcgc ggatctcgga
      601 tatgatcgag agcccgaact tcttgatcag gggcgtgccc gcctcgatga tcaggtggtc
      661 gttctggggc agcgactcaa ggaccttcct catgtggttc ctgtcgacga ggtccatggc
      721 gacctggagg tagggcgggt cccagaggcg ctggaccttg aaccccatga ccgcgtgggc
      781 ggcccggtcc ttctcgtgca ggagcgtctc gacgtcgggg aaccggtcga gggcccggcg
      841 gagcgcgagt ttcatcgcgc cgtagttgaa ccggtagatc ttgttgtagt cctgtgcggt
      901 cgggtggacg aagacgctcg ccatgatgac gatatcctcg atgtcgagcc cctcaaagac
      961 gccctcctcg accgagtcgg cgacggcctt cgccaccgcg gcctgcacgg gcccgaacat
     1021 ctggttcacc tggtactcct tcttcagagt gaccttcggg atgacaagcg tcgagggctt
     1081 tgtcggcagg ttcggcctga tgaccgcaag gagcggtgtg tggcccctcg atagctgcga
     1141 tatcgagttc gcgaacgcct gccctaccgc gccctcttta tttcccatga cgagatcgat
     1201 atgcgcaagt tctgcgccgt ctccaaccaa tgcttcaccg atgagataca tgctcatgcc
     1261 ctcagatagt gcagaaatat tggtgagaca ggtattaaag atcatccggg ccaatccgta
     1321 gaaatcgcga gcgatacagg ttttttcagg gagagccggt gatcaagagg gattttgccc
     1381 cggaagcggt gcggtgtcgc gggcagacgc tttgcgcctg aaaactccgg ccgcgattgc
     1441 aagtttgacc caccgtcctc ctgcagcaca caacgcaggc gacgactcac gcctggagag
     1501 cgccggcacc aggtctaccg ggcttcgatc gcgagagaag atagccggga acacaaaagg
     1561 ctcaacacga tacccggcgg tatccgggag aagtcatgag aatgtggggt cggtgagatt
     1621 tgaactcacg atcgacgggt ctctccgaca tgcatcagtg ctccaacggg tcatcatctc
     1681 tcaatcagga gacccattgt tcatcacgtg caaagaccgc tggagcccgt cgccattccg
     1741 ggcttggcca cgaccccgct ctctatacag tcgacgccat gcagtataag gattgcgctg
     1801 agagaaacca gtaacgatat aactgtaccg catcccattt atatagaatg gttgagggca
     1861 gttacgcgtg tgggaactcg caaaaacccc tgatcggcat caccatcggc gagatgctga
     1921 accgaattgc agcggcgcac ccggacagtg aagcaatcgt ctccgtccac cagggtatcc
     1981 ggtggacgta tgccgagttt ctcgagcgcg tgaacaccct tgcgcgcgcc ctcatggcgc
     2041 tcgacgtgga gcgcggggac cgtgtcgcca tatgggcgct gaactacgcg gaatgggtgc
     2101 tggtccagtt cgccaccgca aagatcggcg ccatcatggt gaacatcaac ccggcctacc
     2161 ggacctacga gttcgagtac gccatgaagc agtccgaggt gcagacgctc ctcctccagg
     2221 ggcggttcaa gacgtccgac tacgtcggca tgttctatga atcctgcccc gaggcgttcg
     2281 aggcaaagcc gggccggatc aacagcgaca aattcccgtt cctaaagaac gtcgtcttcc
     2341 ttggcgatat accctacaac ggcatgtata cctgggacga cctccttgcc agagccgcgc
     2401 tcatcagccc cgatgagctc cgggaacggg aagagtcgct taacttcgac gacgcggtca
     2461 acatccagta caccagcggg accaccggct ttcccaaggg cgtagtcctg acccaccaca
     2521 acatcttaaa caacgggttc atcatcggcg aggggatgaa gttcacccac gaggaccggc
     2581 tctgcatccc ggtgcccttt taccactgtt tcggcatggt cctctccaac atggccgccg
     2641 tcacccacgg ctccgcgatg gtcctgccct cgccggtctt cagcgccgaa gcggtcctcg
     2701 ccacggtaca ggatgagcgg tgcacggcgc tccacggggt gccgacgatg ttcatcgccg
     2761 aactctctca cccggacttc tcgaagtacc ggctggactc gctccgcacc ggcatcatgg
     2821 ccgggtcgcc ctgcccgacc gaggtgatgc gggaggtcaa ccagaagatg aacatgtccg
     2881 agatcgtgat cgtctacggg cagacggaga cctctcccgg cgtcacgatg accacgaccg
     2941 ccgatcccct ggaacgccgc gtctccaccg tcgggaggcc gttcccccac accgagatca
     3001 agatcgtcga cccgcacaca aagaggatcc tcccccgcgg ggagaccggc gagatctgcg
     3061 cacggggata ctgcgtgatg cggtgctact acaacaatcc gaacgccacc cgtgcgacga
     3121 tcgacgagaa cggctggaac cataccggcg acctcgggac gatggacgag gaggactacg
     3181 tcaagatcgt cgggcgcctg aaggatatgg tgatccgggg cggcgagaac atctacccgc
     3241 gcgagatcga ggagtacctc cacaatcacc cgtcggtcgc cgacgtctac gtgatcgggg
     3301 taccggacca gaagtacggc gaggagttga tggcctggat caagctggag aacggggcga
     3361 acctcaccga ggacgaggtg aaggagttct gccggggccg gatcgcgcac ttcaagatac
     3421 cgcgctacgt caagttcgtc gatgatttcc ccatgacggt ctcgggcaag atcatgaagt
     3481 tcaagatgcg ggaggcggcg atcgcggagc tcggccttga gtgcgcgtct cagatcgaga
     3541 ccgcataacg ccctccccaa cttttttacc gcgcacgacg tggtgttcgt atgcatgaga
     3601 ccttcggcat cccggagatt atcgaggcga tccaatcgct cctcaccgac ccgggcgtaa
     3661 accgcctggg cccggatgtt ctggagcctg cctgggagag cccgctcatc ggcgtctcgc
     3721 gcggcgatga cccgctgtac gctgagttcc aggagacgat cgggccgttt cactggacgc
     3781 ccctcgaggc gttctgcacc gaattcccgg atgccggcgt cacggcatcc gatatcgccg
     3841 tcgtcgtctg ggcgctgccg cagaccgccg caacgcggcg cgaccaccgt gcatgcacgg
     3901 ctcttccctc gcggcgctgg agccttgccc gccactacgg agaagcggtg aacgaccgca
     3961 tccgcgacca cctcgcccgg acgctcacca atgccggata cccggccgtc gccccggcca
     4021 ggctcccggt gttccggcga gagacctctg agcggttcgg catcgcctcg cgctggtcgg
     4081 agcggcacgc cgcctatgcc tcgggcctcg gcaccttcgg cctctgcgac ggcctgatca
     4141 cggagcgggg caaggcggtg cgcctcgggt cggtcgtggc cgcgataaat cttcccgcaa
     4201 cgccgcggcc gtatagcaac aggaccgcat actgcctcca cttctccgga gatgagtgcg
     4261 acgcctgcat ccgccgttgc cccgcaggcg cgatctcggc ggacggccac gacaaggagc
     4321 gatgctttgc gtacattcgg gagatgatgg tgcccttcgt cgagagcgat atcggcatcc
     4381 cggtcacgag ctgcggcctc tgccagaccg gcgtgccctg cgaggggcgg cggccgcgat
     4441 aatggggtat tctgccgcgg tggcctcttc atctcccggc cctggcagag ggcggccatg
     4501 accgtgccgg cgcggtgaag aaggatggtg caggccggct ctaccggaga cgtcacgatg
     4561 tcgcccggga tcatcgccga tcgcgatgga tcgggtgcaa tggtagaaag atggcccggt
     4621 gccggcccgg gccaacctat atattgtggg atcgccatcg ggctgcagcg tgggggtgat
     4681 gggtcaggat atgtaccctg ccgtgagtcc ttctccaccg tatccaccct gctcaccggt
     4741 cttttggggc gcggcgtagc gcccctctgc cggccgtatc atcgatctca ccgttctctg
     4801 ccgggatgaa acccggacgc gcacgccctc atgccggagc ctcgccccca cacgagggcc
     4861 ggcgaggttt gcaggttatc aggaggtatc ggtatgaaac gattcatacg tacaatggca
     4921 gttgctgctg ttctggtcat cgccatcgtg cttgctacag gcgcagcctc agcacaactc
     4981 ttcccgcccg gaccgggacc aatgacgccc ggacagggaa cggggcccgg gccgggaccg
     5041 gccggggcga ccgcgatcac gagcgggccg ggggacaacg agtttcctg
//
LOCUS       DAOSEE010000142         5070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_5870_length_5070_cov_214.651047, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000142 DAOSEE010000000
VERSION     DAOSEE010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5870_length_5070_cov_214.651047"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="PLI76_09480"
     CDS             <1..912
                     /locus_tag="PLI76_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HOI62304.1"
                     /translation="GMLTTAEGKVSIGAPMGTYTVNVAKPGYRSWKKEGVEVRIGRTT
                     NLGIVPLEKENFFAEIRVKSPSRMAAIGTNPQYEMTLKNAGRNDDTYALSVEGLPEQW
                     YARFKESRDATEEVSEIYIPAGEEKPLYLDLIPPYSVDVGEYNFTAVIDSSARQYEEN
                     LTLRLRGSYTMRTYVKSYRHEINRGDTLTFDMTVSNAGVGGALTRIGINMSAPEGWRA
                     NVDPASVASLEPGERATVRVTVVPPADIVAGEYKVVALVKSDQAEGEDEFRIVVKEQS
                     YVAILGLLVMVGVAAGLWYMFRRYGRR"
     gene            complement(1015..3813)
                     /locus_tag="PLI76_09485"
     CDS             complement(1015..3813)
                     /locus_tag="PLI76_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOI62305.1"
                     /translation="MLHILVVDNDPALLESTRIFLERSGDLQVEVTPSPLQGLDMLTA
                     APYDAIVAGYEMPEMDGIEFLREVRGRGMGIPFIIFSGCGREEVIIEAINNGADFYLR
                     KGDNPSARLAELRNMILQGIRQRQAEAEVQRSRDMYQSIFEHTGSATIIIEADMTIAL
                     ANSEAARLTGYSREEVEGKMPWTAFVVAEDLRRLSGYHQHRRLDPGSAPRNYEFRLVD
                     REGRKKDIYMTIGSIPGTDRTVASMIDISDRKQYEANLTAAHEELTAALEEVMAGQES
                     LAMQCRELEDYQATLQGIIDFLPDPTYVLDRTGTVTIWNRAIEQVTGIQKSQIIEARP
                     EFLAEAIPGLSTSLLAASILSRKAPEGEIAREISLPSPHTGEEIDLWSKASLLYDAQG
                     GLSGAIESLRDVTELKRMEAQIRRRIDLERMVGSISARFVALDPGNLNDALNETVRTL
                     GSFFGVDRSYIFSFSADLSCAGNTHEWCAEGIEPQIDLLQNLRLDTLPWGMAHLEARR
                     AISIPDIRNLPVEAAAERELFLQYGARSLVLVPIATADEILGIVGFETVRAPRKWSDD
                     DITLLTVVGNLLANLFIRIRAHERLRESEERFRMLVERSHDCYIRATWQPPAIEYISP
                     SCERLTGYTAGECMGIPGFIPDLIHPDDREAFRTLVETPVLPADRPPILRVRRKDGRY
                     AWIEVCTIPVYGNDGHVAAVEYAVHDIDAWKEAEAALLQANKKLTLMNSIVRHDILNQ
                     VTVVLGHIALLREQPLDAAVIAALDKQQAAIETIRSQIEFTRDYQDLGVRAPQWFSVE
                     PLVTAAARALRQNRIRIVTDLNKTSVYADPLLSMVFYNLLENTVRHGKTVTTVRVTAV
                     PDGGGARITWEDDGIGIPAAHKERIFERGFGSHTGLGLFLVREILSITGMGIRETGTP
                     GRGARFEILVPEGCARFE"
     gene            4248..4571
                     /locus_tag="PLI76_09490"
     CDS             4248..4571
                     /locus_tag="PLI76_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOI62306.1"
                     /translation="MLTLSFSTIPELVRLLSLLGNEQRLAILRSIAIDEKYAREIAEE
                     LGISRPLAAIYLRQLEKAGLVEGASRTSDEPPYLRRYYRAVPFEVVVDLNVVLSLERS
                     EDETG"
     gene            4558..4890
                     /locus_tag="PLI76_09495"
     CDS             4558..4890
                     /locus_tag="PLI76_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62307.1"
                     /translation="MKQDRWKLFLKALWGAVIALLVISLIAGLIGGSKFWSTMLAGSV
                     WVIIQLGALTLLLIIVVRIKEWIEKQVANVAEQKAAGTELQMLRQSMERIEEKVDRIE
                     KVLDGISE"
BASE COUNT         1001 a         1505 c         1585 g          979 t
ORIGIN      
        1 gggatgctca cgaccgccga gggcaaggtc tccatcggcg ccccgatggg cacctacacg
       61 gtcaacgtcg ccaaacccgg ctaccggtcg tggaagaagg agggcgtcga ggtccggatc
      121 ggccggacga ccaatctcgg gatagtgccg cttgagaagg agaacttctt tgccgagatc
      181 cgggtgaagt ccccgtcacg gatggcggcg atcggcacca acccgcagta cgagatgacc
      241 ctgaagaacg ccggcaggaa cgacgatacc tacgccctct cggtcgaggg cctccccgaa
      301 cagtggtatg cccggttcaa ggagagccgg gatgccaccg aagaggtctc ggagatctac
      361 atcccggcgg gcgaggagaa acccctctac ctcgacctca taccgcccta ctcggtcgat
      421 gtcggggagt ataacttcac ggcggtgatc gactcctccg cccggcagta cgaggagaac
      481 ctgacgctcc ggctccgggg gtcgtacacc atgcggacct acgtgaagag ctatcgccac
      541 gagatcaacc gcggcgatac cctcacgttc gatatgacgg tctcaaacgc cggcgtcgga
      601 ggagcgctga cgaggatcgg gatcaatatg agcgcgccgg aagggtggcg ggcaaacgtc
      661 gacccggcat cggtcgcgag ccttgagccc ggcgaacggg caacggtgcg ggtgacagtg
      721 gtcccgccgg ccgatatcgt cgccggcgag tacaaggtcg tcgccctggt gaagagcgac
      781 caggccgaag gagaggacga gttccggatc gtggtgaaag agcagtccta cgtcgctatc
      841 ctcggcctcc tggtgatggt cggggtcgcc gcggggctct ggtacatgtt caggagatac
      901 ggccggcggt gaggccaaaa tcccctggac aactttttct gcgagaacta tcacaaacgc
      961 gagcgtccgg agaggttgca ggaacgcgat ccttcgtggt agagcccggt ctcttcactc
     1021 gaaccgggca catccttccg gcaccaggat ctcgaagcgt gcccccctgc ccggcgtccc
     1081 ggtctcccgg atgcccatcc cggtgatcga gaggatctcc cgcacaagga agagcccaag
     1141 acccgtgtgg ctcccaaaac cccgctcgaa tatgcgctct ttatgtgcgg cggggatgcc
     1201 gatgccgtca tcctcccagg tgatccgtgc cccgccgccg tcgggcacgg ccgtgacccg
     1261 gacggtggtc acggtctttc cgtgccgcac ggtgttctcg aggaggttgt aaaagaccat
     1321 cgagaggagc ggatcggcgt agacggaggt cttgttgaga tcggtgacga tccggatccg
     1381 gttctgccgg agcgcccgtg ctgccgccgt gaccaggggc tcgacggaga accactgcgg
     1441 ggcccggaca ccgaggtcct gataatcccg cgtgaactcg atctgcgacc gtatagtctc
     1501 gatggcggcc tgttgcttgt cgagggccgc gatgacggcc gcatcaagcg gttgctcccg
     1561 gaggagggcg atatgtccga gcaccaccgt gacctgattc aggatgtcgt gccgcacgat
     1621 gctgttcata agggtgagtt tcttgttggc ctggaggagc gctgcttcgg cctctttcca
     1681 cgcatcgata tcgtggacgg catactcgac tgcagccacg tgcccgtcat tcccatagac
     1741 cgggatcgtg cagacctcga tccaggcgta gcgtccgtct ttccggcgca cccggaggat
     1801 gggcggccga tcggcaggca gcaccggggt ttccaccagg gtacggaatg cctcccgatc
     1861 atccggatgg atgagatccg ggatgaaccc cggaatgccc atacactccc cggccgtgta
     1921 acccgtcagg cgttcgcacg aggggctgat atactcgatc gccggcggct gccaggttgc
     1981 acggatgtag cagtcgtgtg accgctcgac gagcatacgg aaccgttctt cgctctcccg
     2041 gagtcgttcg tgagcgcgga tccggataaa gagattggca agcaggttgc cgacgaccgt
     2101 gagcagggtg atatcgtcgt ccgaccactt ccggggtgct cgaaccgtct cgaagccgac
     2161 gatgccgaga atctcatcgg cggtggcgat cgggacaagg acaagcgatc tggccccata
     2221 ctgcaggaaa agttcacgct cagcggcggc ttccaccggg aggttgcgta tgtcgggtat
     2281 ggagatcgct ctccgtgcct cgaggtgcgc cattccccag gggagtgtat cgaggcgcag
     2341 gttctgcagc aggtctatct gcggttcgat cccctccgcg caccattcgt gggtgttccc
     2401 tgcgcaggag aggtccgcgg agaagctgaa gatgtaactc cggtcgacgc cgaagaacga
     2461 cccaagtgtc cggaccgtct cgttcagggc atcgttcagg ttgccggggt cgagggccac
     2521 gaaccgcgcc gagatcgaac ccaccatccg ctcgaggtct atccggcgcc ggatctgcgc
     2581 ctccatcctc ttcaactcgg tgacatcccg gagcgactcg atggcgccgg agagcccccc
     2641 ctgcgcatca tagagcaggg atgccttgct ccagagatcg atctcctcac cggtgtgagg
     2701 tgaagggaga gagatctccc gggcgatctc cccttccggg gcctttctgg agaggatgct
     2761 cgccgcaagc agtgaagtag agagtccggg tatcgcctcg gccagaaact ccggccttgc
     2821 ttcgatgatc tggctcttct gaataccggt cacctgctcg attgcccggt tccagatggt
     2881 cactgtcccc gtacggtcga gcacatacgt cgggtcgggg aggaaatcga tgattccctg
     2941 gagggttgcc tggtaatcct cgagctctcg gcactgcatc gcaagcgact cctggcctgc
     3001 catcacctcc tcgagcgccg cagtcaactc ttcatgcgcc gcagtcaggt ttgcctcgta
     3061 ctgctttcgg tcggatatat cgatcatcga tgcgacggtg cgatccgttc ccgggatgct
     3121 cccgatcgtc atgtatatgt ctttctttct accttcccgg tcgaccagcc ggaactcgta
     3181 gtttctcggt gcggagccgg ggtccaaccg ccgatgctgg tggtagccgg aaaggcgccg
     3241 gaggtcctct gcaacgacaa acgccgtcca gggcatcttc ccttcaacct cctccctgga
     3301 gtagccggta aggcgagcag cctcgctgtt cgcgagggcg atcgtcatgt cggcctcgat
     3361 gatgatggtg gcggagcccg tgtgctcgaa gatgctctgg tacatgtccc tcgaccgctg
     3421 cacctccgcc tcggcctgcc tctgcctgat cccctggagg atcatgttcc ggagctcggc
     3481 taaccgggct gaggggttgt cgcccttccg gaggtagaag tccgcgccgt tgttgatcgc
     3541 ctcgatgatg acttcctcgc gcccgcagcc ggaaaatatg atgaacggta tacccatgcc
     3601 gcgcccgcgg acctctcgca agaattcgat gccgtccatc tccggcatct cgtaaccggc
     3661 aacgatggcg tcgtacggtg ccgcagtaag catatcaagc ccctgcaggg gcgatggcgt
     3721 cacctcaacc tggagatctc cggatcgttc cagaaagatc cgggtcgatt cgagaagggc
     3781 cggatcattg tcgaccacaa ggatatggag catgaaccca tcccatgtga agatctgaac
     3841 gatagcaacg ataaatattg tgattttgca ttggcgttcg tatcgaaaga aacaagaaac
     3901 tgtgatttta aaacccacag ataaaagatc cgtgagatct tgccgtacct gcgggcgaca
     3961 ccggtcccgg gaattatcag catgttccgg catcactatg ccgccggcag gatgacgggc
     4021 gtatgtatga tgcgaatctc ttccttgaga tctccgagtc ggaagaggac ccgggcgttt
     4081 gacgatctcg cccgcaaccc gggcgtgcgt gtcttccacc tccgatcaac ccggtggccg
     4141 gatgctatcc cgccctccgg ggcgaggtgg ttcgtaaacc ccaaactccc gaagggagca
     4201 cgggatgccg tatcataatg ttcatatatg taaaaaataa taaacacgtg ttgacactat
     4261 ctttcagcac tatccccgaa ctggtacgac tgctctccct gctcgggaac gagcaacggc
     4321 tcgccatact ccggtccatc gcgatcgacg agaagtacgc gcgcgagatc gccgaggagc
     4381 tcggcatatc gcgcccgttg gccgcgatat acctgcggca gctcgaaaag gccgggcttg
     4441 tggagggagc cagcaggacg tcggacgaac cgccatacct ccggaggtac taccgggcgg
     4501 tgccgttcga ggtggtggtg gacctgaacg tggttctgtc attggagaga tcggaagatg
     4561 aaacaggata gatggaaact cttcttaaag gcgctctggg gcgccgtcat cgcactgctc
     4621 gtgataagcc ttatcgccgg cctgatcggc ggttcgaagt tctggagcac catgcttgcc
     4681 gggtccgtct gggtcatcat acagctcggt gcccttacgc tcctcctcat catcgtggta
     4741 cggatcaagg agtggatcga gaaacaggtg gcgaatgtcg ccgagcagaa ggctgccggc
     4801 acggagctcc agatgctgcg gcagtcaatg gagcggatcg aggagaaggt ggaccggatc
     4861 gagaaggttc tcgatggaat ctcggagtaa aaagtgattg ggagggtgta tggggggcgt
     4921 gcagtaaccg gtctaagggt acggcagacg gggcgcaggc agggatccat cgcgcatgac
     4981 gcctgaccat gtagtaatat gaatccggca tataataata ctatctttta tgacaaagtt
     5041 ttggttcggt agcaacacgg ctgcctgccc
//
LOCUS       DAOSEE010000143         4942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_6045_length_4942_cov_222.369347, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000143 DAOSEE010000000
VERSION     DAOSEE010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6045_length_4942_cov_222.369347"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="PLI76_09500"
     CDS             <1..875
                     /locus_tag="PLI76_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase"
                     /protein_id="HOI62308.1"
                     /translation="LPLDPLYAEKYHFKLIPVSEVVVFEGGGERLDYVPEEAKEQAEA
                     LLDMGIENFKERHSMIEPIRGLPVREAVVGFAPESIVAALGGSLDALLGAIKDGTIRG
                     IVGLVSCTTLRDTGQDVHTVAVAERLIARDILVLGMGCGVAALQVAGLCSPEAKEKAG
                     PGLKGLCTALGVPPVLGFGTCTDTGRCADLLLSVSEALGGVPIPDLPVAVAAPEYMEQ
                     KATIDALFALALGLYTYVNPIPTVTGGPDLVKLLTEDLRWITGGVLRVETDAEAAVEG
                     IAGHIEGRRKILGL"
     assembly_gap    1110..1209
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1210..1480)
                     /locus_tag="PLI76_09505"
     CDS             complement(<1210..1480)
                     /locus_tag="PLI76_09505"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOI62309.1"
                     /translation="MNVRQLLDWHPGGVPGGLSGIRCDSSKGGRELMQQRDAIDWYNE
                     GIALHISGDRDGAIRCYDHALEIDPEYAAIWGSKGIALYELGRYDE"
     gene            1685..2842
                     /locus_tag="PLI76_09510"
     CDS             1685..2842
                     /locus_tag="PLI76_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62310.1"
                     /translation="MSTGALDWLEEHFPRDFFDETVAQRALRHLGFNEKRALFSDDYV
                     LQRTSEGFWYEFIVYEIMLELSLETDRIQSIVGKGADASAYSVGMRPGQNGFYYSERG
                     SLTVRGNGQNIAEMDLLFVDGRGNMGFVEVITSAMELEKFYEEIHYKKTLLASLFGQE
                     TVPFVLVSSVDVSRYDSILRIAEMPDSLILITNPMEEIRTLIYEGSLRRRPEKPGHHP
                     KRIDLSSIRPRRPFDYKEIHDRNRDVVIRTLLSCRDGGIAVISSAINSLSKKIMLGTL
                     GEAGVKAVLRDRKVRIKDTTLYADDIKQRFSRVVIAFDIPAYTPVIYFKLKEKEVYLK
                     LIQNASGDLVFQGKRTPAPYMSGFYEWLDADKPTVDSRVAERYASLFLGTS"
     gene            3134..3829
                     /locus_tag="PLI76_09515"
     CDS             3134..3829
                     /locus_tag="PLI76_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOI62311.1"
                     /translation="MEEGPRGAITQRDRTSYAIVPRTPAGIISPEHLESIIKVVRRYN
                     IPVIKMTSGQRMALVGIKKEDVEKIWNELGMTVGQATAPCVHYVQACPGTVTCKYGKQ
                     DALGLGLEVEELYQDMNLPAKLKMGVSGCPRCCGESYLRDIGLIGDAKGWNVIFGGNS
                     GGRPRIGDVAARGLSKDEALDLVRRLLDYYRKNARPGERTARFVERVGFDTIKSDVLT
                     LAPYIPLDSIRSR"
     gene            4275..>4942
                     /locus_tag="PLI76_09520"
     CDS             4275..>4942
                     /locus_tag="PLI76_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2115 domain-containing protein"
                     /protein_id="HOI62312.1"
                     /translation="MQFRVFLNRLLRLIGRGGRSEPGADPDDACALIIDPFAGDPGEE
                     VGEGSIPVDAIRAACRRMHGAATKGELLRIVASEVAAFDLRDLEAMNAGFERKVANLP
                     PDYRDRLLASVREEIFSAHHRLVLLSRNGSNPGMDEPPGPGHQTYWAMVAEACTAKAR
                     EKDPKYLYLKYLLSGFTMFVLKEPAHPVGTPFPGGQIVDEWEGAYLCPVRDKADDVAF
                     ALCP"
BASE COUNT          954 a         1539 c         1473 g          876 t
ORIGIN      
        1 cgctcccgct cgatcccctc tacgccgaga agtaccactt caaactgatc ccggtgagcg
       61 aggtcgtggt cttcgagggt gggggcgagc ggctcgatta cgtcccggag gaggcaaagg
      121 agcaggcgga ggcgcttctt gatatgggga tcgagaactt caaggagcgg catagcatga
      181 tagagccgat ccggggcctt cccgtgcggg aggcggtggt cgggtttgcg cccgagagca
      241 tcgttgcggc gctcgggggg agccttgatg cgctgctcgg agcgatcaag gacggcacca
      301 tccggggcat cgtggggctt gtctcctgca caacgctgcg ggataccggg caggacgtcc
      361 ataccgtcgc ggtcgcggag agactgatcg cccgcgacat cctggtcctc gggatgggct
      421 gcggggttgc ggccctccag gtcgccgggc tctgctcgcc cgaagcaaag gagaaggccg
      481 gcccggggct caaagggctc tgcacggcgc tcggcgtccc gccggtgctc ggattcggga
      541 cgtgcaccga taccggccgg tgcgccgacc tcctcctctc ggtctcggag gccctcggcg
      601 gcgtccccat ccccgatctc ccggtcgccg tcgccgcacc ggagtacatg gagcagaaag
      661 cgacgatcga cgccctgttt gcgctcgcgc tcggtctcta cacctacgtc aacccgattc
      721 cgacggtgac cggggggccg gatctcgtca aactcctcac cgaagacctc cggtggatca
      781 ccggcggggt gctccgcgtc gagacggatg cagaggcggc ggtggagggg atagcagggc
      841 acatcgaggg gaggcgcaag attctcgggc tgtgaggggc gggacgggca gggggcttct
      901 gctcaaacgg cccctttccg gcgaggcgat tccggtaggt cttcggtgcg tgcgccctta
      961 tatcgaggcc cctctatcac tctcttttgc tgttgcaccc ctctttttca atgcaccttc
     1021 tctgttgctc tttgcgagag tatctccggg gtctatctcc agcgcgtgat cgtagcactg
     1081 gatcgcctcg tcatagcggc cgagttcgtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnc ctcgtcatag cggccgagtt cgtagagcgc aatgcctttg ctgccccata
     1261 tcgcggcata ctccgggtcg atctccagcg cgtggtcata acaccggatc gccccgtcac
     1321 gatcccctga aatgtgaaga gcaatgccct cgttatacca gtctatcgca tccctttgct
     1381 gcatgagttc tcttcctcct ttgctgctgt cgcaacgtat gccggataga ccacccggaa
     1441 caccgccagg gtgccagtcg agcagttgcc tgacgttcat tcgttgttac ccggcgagca
     1501 taaccctatc cattacgccg ccggcgcatc ggggcatcct cgctgagcaa caaccatggc
     1561 aacaaatcgc atgatgaatc tgtctccctc atcggtagtt ccccggagca ctacccaccc
     1621 tgtacccccc tgcccgggcc ggccggacga aaatagtaat atacctccag tggtatccta
     1681 tcatatgagc acgggcgccc tggactggct tgaagagcac tttccccggg atttttttga
     1741 cgagacggtt gcacaaagag ctcttcgcca tctcggattt aatgagaagc gtgccctctt
     1801 ctccgacgac tacgtgctcc agcgcacgtc cgaggggttc tggtatgagt tcatcgtgta
     1861 cgagatcatg ctggagctct ccctcgagac cgaccggata cagtccatcg tgggaaaggg
     1921 agccgatgcg tcggcatact ctgtcgggat gcgccccggg cagaacgggt tttattactc
     1981 ggagcgaggg agcctgaccg tcagaggaaa cgggcagaac atcgccgaga tggacctcct
     2041 gttcgtggac ggccgtggga acatgggctt tgtcgaggtg atcacctccg cgatggaact
     2101 ggagaagttt tatgaagaga tccactacaa gaaaacgctg ctcgcctctc tgttcgggca
     2161 ggagaccgta cccttcgtcc tggtctcgtc ggtggatgtc tccaggtacg acagcatcct
     2221 caggattgca gagatgcccg acagtctcat cctgatcacc aaccccatgg aagagatacg
     2281 aaccctcatc tacgaggggt cgctgcggag gcgcccggag aaacccggcc atcacccaaa
     2341 acggatcgat ctcagcagta ttcgcccgag aagacccttc gactacaaag agatccacga
     2401 ccgcaacagg gatgtggtca tcagaaccct gctctcctgc agggatggcg ggattgctgt
     2461 gatatcatct gccatcaact cgctctcaaa gaagatcatg ctgggcacgc tgggggaggc
     2521 cggcgtcaaa gcggtgctgc gcgacagaaa ggtcaggata aaagatacca ccctgtatgc
     2581 cgacgacata aaacaacggt tcagcagggt ggtgatagcg tttgacattc ccgcgtatac
     2641 gcccgtcatt tacttcaaac tgaaagaaaa ggaggtgtac ctgaaactca tccagaacgc
     2701 ttccggagac ctggtgttcc agggtaagcg gacgcctgca ccctacatgt cggggttcta
     2761 cgagtggctg gatgccgata agcccacggt cgactcccgg gttgcagagc ggtatgcctc
     2821 gctctttctg ggcacaagct gaacgaaaat tcccggggaa cgcatctttg aaaaaaccac
     2881 acgggatccc tctcatatac gccggccggc ctcctcgccc tcccgaaaat cccagaaaac
     2941 ctgttcgccg cgaaggtcgt gcccctgacg gcaaccgcat acctcccgca cccccgctca
     3001 cggaacagag gaccatacgg ggatccctga ggggacgggc cttagagagc agcggcactc
     3061 ttttccctca ggagaacgaa ggttggatca tatccggcgc acctgacttt gccggaaaaa
     3121 cgagaggcat actatggaag aaggaccaag gggagcaatc acccagagag acaggacatc
     3181 ctacgcgatc gtgccgagaa cccctgcggg aatcatcagc cctgagcacc tggagagcat
     3241 catcaaggtc gtcaggcgct acaacatccc cgtcatcaag atgacgtcgg ggcagcggat
     3301 ggcgctcgtg ggcatcaaga aagaggatgt cgagaagatc tggaacgaac tcgggatgac
     3361 cgtcggccag gcgactgcgc cctgcgttca ttacgtccag gcatgccccg ggacggtgac
     3421 ctgcaaatac ggcaagcagg acgccctggg cctcgggctt gaggtcgagg aactctacca
     3481 ggatatgaac ctgccggcaa aactcaagat gggagtctcc gggtgccccc ggtgctgcgg
     3541 cgagagttac ctccgcgata tcgggctcat aggggacgca aaaggctgga acgtcatatt
     3601 cgggggcaac tccggcggcc ggccgcgtat cggcgacgtc gctgccaggg gcctctcgaa
     3661 agatgaagct ctcgacctcg ttcgccggct gctcgattac taccggaaga acgccagacc
     3721 gggagagcgg accgcacggt tcgtcgagcg ggtcgggttt gacaccatca aaagcgatgt
     3781 cctgaccctc gctccctaca taccgctgga ctccatccgg tccagataag gggttgcgcg
     3841 gccccactcc catctccccg ggccgccgac ttgcccggcg ttccggaccc cgcccggccg
     3901 ggagacgatg cgttgagcga tctgagaatg gagcgcgaac acccctccct catcatcccg
     3961 ggcgccgggc gatccccgca aagaccttct tgaacggccg ggacagcagc cccatagtcg
     4021 cgaggcccgc cggcggtcga gaggccccgc catgggtatt catggagcgg gcccggatgt
     4081 aacatcggcc agaccctctg tcactcccgg caggcgacca tgcaggcccg cgggcgggca
     4141 cccgcctcgc cgatcccggc aaaggccgca tggcctcgta cccgcgagcc gggttatcca
     4201 cactcttacc cggggattac gccaggatta tacctcttca cgccccatct tttccagaga
     4261 actcgggtga catcatgcag ttccgcgtgt tcctgaaccg tctcctgcgg ctcatcggcc
     4321 ggggaggccg gagtgagccc ggcgccgacc cggacgatgc ctgcgccctc atcatcgacc
     4381 ccttcgccgg cgaccccggt gaggaggtcg gcgagggatc gataccggta gatgccatcc
     4441 gggcggcctg ccggcggatg cacggggcgg cgacgaaggg agaactcctc cgcatcgtcg
     4501 ccagcgaggt agcggcattc gacctgcgcg atctcgaggc gatgaacgcc gggtttgagc
     4561 gcaaagtcgc aaacctcccc ccggactacc gtgaccgcct cctcgcaagc gtcagggagg
     4621 agatcttctc ggcacaccac cgcctggtcc tcctctcccg gaacggcagc aatcccggca
     4681 tggatgagcc tccgggcccg gggcatcaga cctactgggc catggtggcc gaggcctgca
     4741 ccgcaaaggc gcgggagaaa gacccgaagt acctctacct gaagtatctc ctctccgggt
     4801 tcaccatgtt cgtcctcaaa gagcccgccc acccggtcgg caccccgttc cccggcgggc
     4861 agatcgtcga cgagtgggag ggggcttacc tctgcccggt cagggataag gccgacgacg
     4921 tggctttcgc cctctgcccc ta
//
LOCUS       DAOSEE010000144         4887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_6128_length_4887_cov_232.426118, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000144 DAOSEE010000000
VERSION     DAOSEE010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6128_length_4887_cov_232.426118"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            556..759
                     /locus_tag="PLI76_09525"
     CDS             556..759
                     /locus_tag="PLI76_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62313.1"
                     /translation="MAATKRIPVSEEVWAEISELKRPGQTFDDLLSQMAEQEKKRRFI
                     EDMDRIEAEGDFVELEFDVPDTD"
     gene            815..1021
                     /locus_tag="PLI76_09530"
     CDS             815..1021
                     /locus_tag="PLI76_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62314.1"
                     /translation="MEQLKTLQEDPYPGSGSDKERLHGREDIYRLHTSRTFTAFYRIH
                     EPEKEVHVLAVMTIGQAHKRYGWL"
     gene            1230..2417
                     /locus_tag="PLI76_09535"
     CDS             1230..2417
                     /locus_tag="PLI76_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62315.1"
                     /translation="MLPVGSPATGDDFVDRERETAFILNTIKNDHVMLVAPRRFGKTS
                     IMRKIERDLASRGQSCVFLEVESVNSPGEFITDLVTALVECEGASPGARISSALGKAF
                     ALVQEKIDEIETPGFRAKLRGRLRAEFSDDWHEKAKRVDEIFAGLTGPICIILDEFPV
                     AIQNMEEDDARKFLQWFRRLRQVSPGARFIVGGSVSIDHVVYHIGGTPVINDFRRVTI
                     GGFSEDVALSVVERVFQGERWEYSPEIGRAVLDCVGEPCIPYFLMIVLSGLKEEADIS
                     GGVPSPALVEKVYNRRILGSEGRHYFEHYSRRLQTTYSGQEAKAARAILSRVSLAESL
                     PAGIAYEIFRQSTASESEDAFRALLAQLNHDFYVTSETPGELRFYSKMLRDWWRIYHA
                     GAW"
     gene            2401..4059
                     /locus_tag="PLI76_09540"
     CDS             2401..4059
                     /locus_tag="PLI76_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOI62316.1"
                     /translation="MPAPGRCYRYFPAALAGETLRHLLVGRQEIFESLFDEADRASAS
                     RTPRFFLLVGDRGAGKSHLMSLLYRRIRDELSGRMIPVDLAGEEYSIFRASDFFLRVL
                     AGMGVETADVAALGEDSMVREAAVERIAASAGERQVVIFVENIHEVFSQMERREVRAL
                     RSVFQRRDDLSVVASAPSLFSGVLDHEEPFYNFFRVFHLRELDCDEAKDLMRRVAEVD
                     GDTAFIENFGDYEPGIEALRHLVGGNPGLMVRLYEILSQCGAGKVAEAFFRLADEQTP
                     YYREVFRRLPGQRRLILDTILAAETPLTPKEVAGRARLNLATVNAQLRRLEAEGYVVS
                     RPMKKRTTYEARDRLFRMWRAMRQPAGRDRVFALIGFLEAWHGRPAAVDVSYRGAPGV
                     MEGVAPPNRIAAGGREDVLAALQEAEGQTDDPGRFVHLALDLAGEELLVGNRANGLSL
                     IRAAYAHAGSLDPATARRMTAGFLKRLAGAGEVSAVGSAVREIVASGGAGWKGLLKPV
                     TDAVAIVEAKDTRLYYTRLQPEERAVVAGIVRAITGSEELGPGG"
     gene            4386..4787
                     /locus_tag="PLI76_09545"
     CDS             4386..4787
                     /locus_tag="PLI76_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOI62317.1"
                     /translation="MKIILTTVFVNDQDRALKFYTETLGFVKKSDVSAEGYRWLTVVS
                     PDDQDGTELLLELNDNPVARTYQKGMFEQDLPAASFGVEDTRAEYERLKAQGVKFTLE
                     PMEVVAGVTIAVLDDTCGNLIQIQTLQGLST"
BASE COUNT          962 a         1454 c         1523 g          948 t
ORIGIN      
        1 ccccggcagg ggctcttgca gcacggaccc cagttttgca cctgcttaca gcacgttgca
       61 accggcgatg caacttaacg gacgatactc taacgcccta tccgggtttc atcccccgga
      121 ctgccgtatc gtgggatcac ttccagcatt ccggatctcg cacacacacg cacccgcacc
      181 catcccggca ccggtgccgg ggactgtgtg tgttttctgc tgtaatttag tactaagtga
      241 gagacgcatc tctctatctg tacaagtaat gctggatcca gcacctttcc cggccgtttg
      301 atctttgcaa ccggtgctgg aatcctgcgg taaacctgct gcaatgttgt aacttgcaga
      361 tcgcacctat cagtgctcaa actccgtacc ataggaacgt cgtccctatc ggtgctcgaa
      421 ctccggccca ttggcccatc gttcctgcgg tgctcccact cctgctcttc ccttgaactg
      481 ttagaactct ctcgcgtttt caccggctca ctcgcgtaca acactgtaaa tacaacaagc
      541 acttacaata ttatcatggc tgcaaccaag aggattcccg tctcggagga ggtatgggcg
      601 gagatctccg agctgaaacg tcccggccag accttcgacg acctcctctc gcagatggcc
      661 gagcaggaga agaagcgccg gttcatcgag gatatggacc gcatcgaggc cgaaggggat
      721 ttcgtggagc tggagtttga cgttccggat actgattaac agaaaggccc ttgagagtat
      781 ccggagcctc agcgaaaagg gccggcggat agtcatggag cagctgaaaa ccctgcagga
      841 agacccgtat cccggcagcg gaagcgacaa ggagcgcctg cacgggcgcg aagacatcta
      901 tagacttcat accagccgga cattcacggc attctaccgt atccatgaac ccgaaaaaga
      961 agtccatgtc ctcgccgtga tgacaatcgg gcaggcgcac aagcggtacg gatggctctg
     1021 acggcctttc attctatcaa ctgttccagc gcttctcctg ctcccggata atgatcacct
     1081 ccgcccccgg ggtcgcctgc tgtcacggcc cgttgtcgtt cttcctgccc gggtgccgct
     1141 gtatataccg gcattgagat agtgcccgaa caataacgaa tttgttatat aacagatttg
     1201 ttatatacta tatctgtcag gtgtcgtaca tgctgcccgt aggaagcccc gcaacaggcg
     1261 acgacttcgt cgaccgcgag cgcgagaccg cgttcatcct gaacaccatc aagaacgacc
     1321 acgtcatgct cgtcgccccc cggcggttcg gcaagacctc gatcatgcga aagatcgaga
     1381 gagacctcgc gagcagaggt caatcgtgcg tattccttga ggtcgagagc gtcaactcgc
     1441 cgggggagtt catcaccgat ctcgtcacgg cgctcgtcga gtgcgaggga gcgagccccg
     1501 gagcccggat ctcctcggcg ctcgggaagg cgtttgccct ggtgcaggag aagatcgacg
     1561 agatcgagac cccaggcttc agggcaaaac tccggggccg gctgcgagcg gagttctcgg
     1621 acgactggca cgagaaggca aagagggtgg acgagatctt tgcagggctc accgggccga
     1681 tctgcatcat cctcgacgag tttccggttg ccatccagaa catggaggag gacgacgcga
     1741 gaaagttcct gcagtggttc cggcggctgc ggcaggtctc gcccggggcg aggttcatcg
     1801 tcgggggctc ggtcagcatc gaccacgtcg tctaccacat cggcgggacc ccggtcatca
     1861 acgacttccg gagggtcacc atcggcgggt tcagcgagga tgtcgcgctc tccgtcgtcg
     1921 agcgcgtctt tcaaggagaa aggtgggagt attctccgga gatcgggcga gcagttctcg
     1981 actgcgtggg cgagccctgc atcccctact tcctcatgat cgtcctctcc ggcttaaagg
     2041 aagaggcgga tatctccgga ggggtgccct ccccggcgct ggtcgagaag gtctacaaca
     2101 ggaggatcct cggcagcgag gggcgccact actttgagca ctactcgcgc cgcctccaga
     2161 cgacctactc cgggcaggag gcaaaggctg cccgtgccat cttaagcagg gtgagtctcg
     2221 cggagtccct gcccgcgggt atcgcctacg agatcttccg gcagagcacc gcatcagaga
     2281 gcgaggatgc atttagagcg ctgctggcgc aactgaacca cgacttctac gtcacctcgg
     2341 agacccccgg cgagttgcgg ttctactcga agatgctccg ggactggtgg aggatctacc
     2401 atgccggcgc ctggtaggtg ttaccgctac ttccccgcgg cccttgcggg ggagacgctc
     2461 cggcaccttc tcgtcggccg gcaggaaatc ttcgaaagcc tgttcgacga agcagatcgg
     2521 gcatccgcga gcagaacgcc gcggttcttc ctcctggtcg gggaccgggg cgccgggaag
     2581 tcccacctga tgagcctgct ctaccggagg atacgcgacg aactctccgg ccggatgatc
     2641 ccggtagacc ttgcaggaga ggagtactcg atattcaggg cgtccgactt cttcctccgg
     2701 gtgctcgcgg ggatgggggt cgagaccgcc gacgtggccg cgctcggcga ggattcaatg
     2761 gtccgcgagg ccgcggtcga gcggatcgcc gcatcggccg gggagaggca ggtcgtaatc
     2821 ttcgtcgaga acatccacga ggtcttttcc cagatggaga ggcgcgaggt ccgggcgttg
     2881 cggtcggtat tccagcggag agatgacctc tcggtcgttg cctcggcgcc gtcgctcttt
     2941 tccggggtcc tcgaccatga ggaaccgttt tacaacttct tccgggtctt tcacctccgg
     3001 gagctcgact gtgacgaagc aaaggatctc atgcggaggg tcgcggaggt cgacggcgat
     3061 accgccttca tcgagaactt cggggactac gagcccggca tcgaggcgct ccggcacctc
     3121 gtcggcggca acccggggct gatggtccgg ctgtacgaga tcctctcgca gtgcggggcg
     3181 ggcaaggtgg cggaggcctt cttcaggctg gcggacgagc agacgcccta ctaccgggag
     3241 gtcttccgga ggcttcctgg gcagcggcgg ctcatcctcg atacgatcct cgccgcagag
     3301 accccgctca ccccaaaaga agtcgcaggg cgcgcccggc tgaacctcgc gacggtgaac
     3361 gcgcagctcc ggaggctgga ggccgagggc tacgtcgtct cccgcccgat gaagaagagg
     3421 acgacctacg aggcccggga ccggctcttt cggatgtggc gggcgatgcg gcagccggcc
     3481 ggccgtgacc gggtgtttgc cctcatcggg tttttagagg cctggcatgg gcggccggcg
     3541 gccgttgacg tatcataccg gggggcgccg ggcgtcatgg aaggcgttgc cccgccgaac
     3601 cggatcgcgg ccggagggcg ggaggatgtg ctcgcggcgc tgcaggaggc ggagggacaa
     3661 actgacgatc cgggccggtt cgtgcatctc gcgctcgatc tcgcggggga ggaactgctg
     3721 gtcgggaacc gggcaaacgg cctctcgctg atccgggcgg cgtatgcgca cgccggcagc
     3781 ctggacccgg cgacggcacg aaggatgacg gccgggttcc tgaagcggct cgccggggcg
     3841 ggggaggtct ccgcggtcgg gagcgccgtc cgcgagatcg tcgcgtccgg gggcgccggg
     3901 tggaaggggt tgttgaaacc ggtcacggat gccgtggcga tcgtcgaggc gaaggatacg
     3961 aggctgtatt acacgaggct gcagccggag gagcgggcgg tcgttgccgg gattgtccgg
     4021 gcaatcacgg ggtcggagga gctggggccg gggggttagg ggtttggggc ggggtcttcg
     4081 gttcccgctc ggttcgtgtt gtttcctgga ttcccgacaa tacgtaacga ttccaaccga
     4141 tccacgccat tattgccctt aaatctcaat catccttcga aatcaaagca atcaatttac
     4201 atctcgttat gtgttacaca taacgagcat gtttttgcca atccacaaaa cacattctat
     4261 ttcggatttt aattgaaata cggccagttt gcgtacagct cgttatgagt tccgggaatg
     4321 taggaggcat gctccagaca actggttgcc gaaacctaca tgtggttctc ctgatatgaa
     4381 cgaccatgaa gatcatactg accaccgtat ttgtaaacga tcaggacaga gccctgaaat
     4441 tctacaccga gacgctgggt ttcgtgaaga agagcgatgt ctctgccgaa ggctaccggt
     4501 ggctcaccgt cgtctccccc gacgatcagg acgggaccga gcttctgctt gaactaaacg
     4561 acaaccccgt agcccggaca taccagaaag gaatgttcga acaggatctc cccgccgcgt
     4621 ccttcggcgt cgaggacacc cgtgcagaat atgagcggct caaagcacag ggcgtgaagt
     4681 tcacgctgga gccgatggaa gtcgttgccg gggtgaccat agcggtcctc gacgacacct
     4741 gcggcaacct catccagatc cagacactgc aaggattatc tacataaatt ggaggcggcc
     4801 catcccccgg actgctggaa cccctctgtt tttcaccggt tcgcctgttt ccaacattgg
     4861 atgggctgat cccccatcgc cgcgaaa
//
LOCUS       DAOSEE010000145         4881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_6139_length_4881_cov_264.818690, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000145 DAOSEE010000000
VERSION     DAOSEE010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6139_length_4881_cov_264.818690"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PLI76_09550"
     CDS             complement(<1..256)
                     /locus_tag="PLI76_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62318.1"
                     /translation="MKRMFGILALMVIAGAMIAGCVQPGGNETAAPGTTTPGATGTPA
                     ATTATPVGTTPASTANATETPPAPPAAGGESVSITDRGFVP"
     gene            complement(604..1641)
                     /locus_tag="PLI76_09555"
     CDS             complement(604..1641)
                     /locus_tag="PLI76_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62319.1"
                     /translation="MPFPFEVPFEEVQSDLESYIDATFDSLESAFLTMPRGEGFIEYP
                     TFEEGYEALKRATRNFQDLSPESVTAAVYDAPIVFIVLRTILGFTPPEWAYVTTQRTG
                     REVTQGAARAIERKIRTNPFEPLCAANHGITDIRIQAMVRAACELLHEGPRENIAGVL
                     HRLDKADTRQGLNSLQSLAGLGVPYPMLLYERFLGRPFAAHRDSVSGLIGDVLEAAIE
                     DVLSRGGISFRKTKRAERVIGFDQAPDFIIPDEFNPRIVIEAKIAEDDGTARDKVTRV
                     QHLSELGMQGRRTGEAPKYEVIACIAGRGFGIRREDMKKLILSTRGKVFTLGSLDRLI
                     ECTGLKEYTAG"
     gene            complement(1625..3673)
                     /locus_tag="PLI76_09560"
     CDS             complement(1625..3673)
                     /locus_tag="PLI76_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62320.1"
                     /translation="MSEFGGRPVADTFFQANSLPGRTIADPFMGGGTPLLEANRVGCD
                     IVGFDINPMATWIVREEIEHLDLQEYRREAERVITSLEQEIGELYRTDCPLYGESGVP
                     VKYFLWVKTLDCEECGTSVDLFPGYLVAKDARHPHNVVVCPCCGELNEIGDLQAPGAC
                     GCCGERLLSTGPARRNRCICPNCGHQNTYPRPESGPPRHRLFAIEYHNPHRTDGHEGR
                     FFKKPDEKDLAVVQEATRRLRSLCPSYIPAQNILAGDETDRLHHWGYNHYRELFGERQ
                     LLGLELSCRRIAGVPDDRIRRALATNLSDLLRYQNLLCRYDTTALKSLDIFSIHGFPV
                     GLIQCESNLLGIANGQGMNVGSGGWRNIVNKYTAAKQYCEAPFETYQKGKRKIRVPIP
                     GEWIGEYSGTGNRREVHLHCASSAGVKLKPASLDAVFTDPPYFGNVQYGELMDFCYVW
                     LRGLAGTGSEGFDRESTRSPEELTGNESQGRDLEHFADGLSSVYCTMADALKPGAPLA
                     FTFHHNNVETYYTIGVAILDAGLRCLASLPCPAEMGGSLHIHGTKSSIVDTVFVCRRY
                     GYTRRGQDFETPVQLTGIVQDELEQLKRAGMRPTEGDIRCIVYGHLTRMTVERLSGEW
                     NSTLPVGKRLNRFAEAFSSFGDLQPVLDLLTRRLTSATPCNLQVQTRLYEEDRDAVSL
                     "
     gene            complement(4266..>4881)
                     /locus_tag="PLI76_09565"
     CDS             complement(4266..>4881)
                     /locus_tag="PLI76_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62321.1"
                     /translation="TGTLLTGSSGTVLKSVIVNAADNVGSMFVPIGTTALDANGKPLG
                     EIDLKPLAGDAVPAVPSGSVFKFAGYAYEAGPDGATFDPAITLSLDIPEDVWNSLDLT
                     NQQCMMKWYNKESGLWEDVPTTIIPGTRTVEIRITHFSIYALFTEPVTTPTTPTETVT
                     TAPPATPTTPTAPPAEGLSMTMILAIFAVIAIVVVAGYFLMMRK"
BASE COUNT          951 a         1422 c         1441 g         1067 t
ORIGIN      
        1 ccggcacgaa ccctctatcg gtgatggaga cgctctcgcc gccggctgcc ggcggtgcgg
       61 gcggggtctc cgtcgcgttc gcggtggagg ccggtgtggt tcccacgggt gttgccgtcg
      121 ttgccgccgg tgtcccggtg gcacccggcg tcgttgtgcc gggagccgct gtttcgttgc
      181 cccccggctg cacacaccct gcgatcatgg cgcccgcgat caccatgagg gcaaggatac
      241 caaacattct cttcatgggg cagacgatac ttcttggcgt agataaagtt tacccctggt
      301 gcgagagccg ggatgcaggg taaccgtggt gtcggagggc ctcctgcaac tctgccatcc
      361 ggccgataac acgagctgtt ttgtcgatac gcaccctggg cgggtcaaac tcccggatga
      421 cgggctatgg gcaaggggct ccgtatggcg gcgcggtgca gcgatgccgg atcatgcggt
      481 gctggcgggg tgtcgggccg cgccagtttg gagtcctggt tttcagattg ccggcccggg
      541 tgctgaatca tttgtccggg gggcttctga gaaagagccg ggaggcgtcg tgacgaaccc
      601 gcttcatcct gccgtatact ctttcaatcc tgtgcattcg atcaggcggt ccaggctacc
      661 cagggtgaaa accttcccgc gagtcgagag aatgagtttc ttcatatcct cccgacggat
      721 accgaaaccc cggccggcaa tacaggcaat tacttcatac ttcggggcct cgccggtgcg
      781 gcggccctgc atgccaagct cgctcaggtg ctgcacccgg gtcactttgt cgcgcgccgt
      841 accgtcgtcc tcggcaatct tcgcttcgat gacgatccgg gggttgaatt catccgggat
      901 aatgaaatcc ggggcctggt cgaacccgat cacgcgctct gcgcgtttcg ttttccgaaa
      961 acttatccct ccgcgcgaca acacgtcttc gatggcggcc tccagcacat ccccgatcag
     1021 cccggagacg gagtcgcggt gtgcggcaaa aggtctcccc aaaaaccgct cgtagaggag
     1081 catcgggtag gggacgccaa ggccggccag tgattggagg ctgttcagtc cctgcctggt
     1141 atcggcctta tccagccggt gcagtacgcc cgcaatgttc tcgcgagggc cttcgtggag
     1201 taactcgcag gcagcccgta ccatcgcctg tatccgtata tccgttatgc cgtggttagc
     1261 ggcgcacaac ggctcaaatg gattggttcg gatctttcgc tcgatcgcgc gggcagcgcc
     1321 ctgagtcacc tccctacctg ttctctgcgt ggtgacatat gcccactcgg gaggggtgaa
     1381 gccaaggatc gtccggagca cgatgaagac gatcggggca tcatataccg ctgcagtcac
     1441 cgattccggc gacaggtcct ggaaattgcg cgtggcgcgc ttcagtgctt cgtaaccctc
     1501 ttcgaacgtg gggtattcga tgaatccttc tcccctgggc atcgttaaga acgccgactc
     1561 cagagaatca aatgttgcat cgatgtaact ctccagatcc gattgcacct cttcaaacgg
     1621 cacctcaaag ggaaacggca tcgcggtcct cctcgtagag acgggtttga acctggagat
     1681 tgcagggagt tgcagatgtc aggcgccggg taagcagatc caggaccggt tgcagatccc
     1741 caaaactcga gaaagcctct gcgaaccggt tcaaccgctt gccgacaggt agcgttgagt
     1801 tccattctcc agacaatctc tctacggtca tacgggtgag gtgcccatat acgatgcacc
     1861 ggatatcccc ctcggtgggt ctcatgcctg ctcgtttcag ttgctctaat tcgtcctgta
     1921 cgatacctgt cagctgtacc ggggtctcga agtcctggcc tctccgggta tacccatacc
     1981 tccgacagac aaaaacggta tcgacgatcg acgatttcgt gccgtggata tggagagaac
     2041 cccccatctc ggcagggcag ggcaggctcg caagacacct gagcccggca tcgagaatcg
     2101 caacgccgat ggtatagtat gtttcgacgt tgttatggtg aaatgtaaaa gcaagcggtg
     2161 ctcccggttt cagggcatct gccatggtac agtaaacaga cgagagacca tccgcaaaat
     2221 gttccagatc tctgccctga ctctcgttcc cggtcaactc ttccggagag cgggttgact
     2281 ccctgtcaaa cccttctgaa ccagttccgg caagaccccg caaccagaca tagcagaagt
     2341 ccatcaactc gccgtactgg acattcccga agtacggggg atcggtgaac accgcatcca
     2401 ggcttgcggg ctttagcttt acgccggcgg aacttgcaca gtgcagatgc acctcccgcc
     2461 ggttgccggt accggaatac tctccaatcc attcgcccgg aataggcacg cgaatctttc
     2521 gttttccctt ctgataggtt tcgaagggag cttcgcagta ctgcttggcc gccgtatatt
     2581 tgttgacgat gtttcgccat ccgcccgaac ccacgttcat gccctgcccg tttgcaattc
     2641 ccaggagatt tgattcgcac tggatcaacc cgacaggaaa tccgtggata gaaaagatat
     2701 ccagggattt cagagccgtg gtatcatagc ggcacagcag attctggtac cggaggagat
     2761 cggacagatt tgtagcaaga gcccgtctta tgcggtcatc cggaaccccg gcgatgcgac
     2821 ggcagctcaa ctccaggccg agcaactgcc gttcgccgaa taactccctg tagtggttgt
     2881 aaccccaatg gtggagcctg tcggtctcat ctcccgccag gatattctgc gccgggatgt
     2941 agcttggaca aagcgatcgt aatctccggg tggcttcctg caccacagcc agatcctttt
     3001 catcgggctt cttgaagaac cggccctcat gcccatccgt cctgtgcggg ttatgatact
     3061 caatcgcaaa caaccggtgg cgcgggggac cggactccgg ccggggatag gtattctggt
     3121 gtccgcagtt cgggcagata caccggttac gccttgcagg gccggtcgag agcaatctct
     3181 ccccacagca cccgcatgca cctggtgcct gcagatcgcc gatctcgttg agctcaccac
     3241 agcacgggca caccacgacg ttatggggat gccgtgcatc ctttgcaacc aggtatccgg
     3301 ggaacaggtc gacggatgtc ccgcactctt cgcagtcgag tgtcttgacc cagagaaagt
     3361 actttacggg aacaccgctc tccccgtaaa gagggcagtc ggtcctgtag agttccccta
     3421 tctcctgctc caatgatgtg attactcttt cggcttcacg ccgatattcc tgaaggtcga
     3481 ggtgttctat ctcctcgcgt acaatccagg tggccatggg gttgatgtcg aaacctacga
     3541 tatcgcatcc tacacggttt gcttcaagaa gcggagtgcc tcctcccatg aacggatcgg
     3601 caatcgtacg gccggggagg ctgttagcct gaaagaacgt atcggcaacc gggcgcccac
     3661 cgaactccga caagatcaaa ccgcggaaca acgtgccggg ccttctggca aaccacttat
     3721 gaaccgcgat aaccggccga taattctgct gaatctgttt ttctttgagt gccattgcgg
     3781 caatcagtgg tatgtcaaaa ttttcttcta tactcattgt tcccccttcg catgaaaatg
     3841 ccctcctcct ttttcatccg gtatgcatgt ggccctttgg gcatcatgcg gtttttaccc
     3901 cggatgatcc ggcagggcat gttttcgggc gtgtcgctac gatccggctg atttcgcacg
     3961 gctcggacag gagacggaga gatacctggc gaggcggatt atggcatccg tccaagttat
     4021 gatctcgata ctctacgatt tagatgttgc cacgtcggat agggaagagc gaggtccgga
     4081 acggcgacac atctccatat aagctccggc ggtatcggtc atatcccatg aacagtcgtt
     4141 catgttgaga gaagatgtcc ctctcccttc cggtgcgacg gggtcgtgcg atgcccgggg
     4201 atctcacccg atgagcattt gcggaacgtt tacaggtccg gggtcaaaaa aaagaggttt
     4261 tcaaattact tccgcatcat gaggaagtat ccggccacca cgacgatggc gatcacggcg
     4321 aagatcgcga ggatcatcgt catggacagc ccttcagcgg gcggcgccgt gggtgtcgtg
     4381 ggtgtcgcgg gcggtgccgt ggtcaccgtc tcggtcgggg tggtgggggt cgttaccggt
     4441 tcggtgaaga gcgcgtagat actgaagtgc gttatccgga tctcgacggt cctcgtgccc
     4501 gggatgatgg tcgtcgggac gtcctcccag agaccggact ctttgttgta ccacttcatc
     4561 atgcactgct ggttcgtgag gtcgagggag ttccagacgt cctcggggat gtcgagcgag
     4621 agcgtgattg ccgggtcgaa ggtcgcgccg tcggggcctg cctcgtaggc gtagccggcg
     4681 aacttgaaga ccgatccgga cggaaccgcc gggaccgcgt cgccggcgag gggtttgagg
     4741 tcgatctcgc cgagcggttt gccgttggcg tcgagcgccg tcgtgccgat cgggacgaac
     4801 atgctcccga cgttatcggc ggcgttcacg atgacggact tgaggacggt gcccgagctg
     4861 ccggtcagga gggtgccggt c
//
LOCUS       DAOSEE010000146         4878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_6145_length_4878_cov_139.614970, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000146 DAOSEE010000000
VERSION     DAOSEE010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6145_length_4878_cov_139.614970"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PLI76_09570"
     CDS             complement(<1..406)
                     /locus_tag="PLI76_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HOI62322.1"
                     /translation="MEIDETHPFNLAPAEAVRLQETLRTRISLSGDIGEVTLLAGADA
                     SYARGSNEIHAVAVALRYPGLAVVERVSASIETSFPYIPGLLTFREGPALLEAFRRLR
                     SEPDVILFDGQGIAHPRGLGIASHMGILLDRPT"
     gene            547..747
                     /locus_tag="PLI76_09575"
     CDS             547..747
                     /locus_tag="PLI76_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOI62323.1"
                     /translation="MARWVCSVCDYEYNEEAGDPDIGIPPGTLFEDLPADWRCPRCTV
                     GKGAFVRADEEEEVKTDEEDDL"
     gene            821..2347
                     /locus_tag="PLI76_09580"
     CDS             821..2347
                     /locus_tag="PLI76_09580"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOI62324.1"
                     /translation="MPGASALPLVDAVRRNRNARYIVVRHEQTAALAASAYNKFTGKI
                     AVCLTIAGPGATNLATGLYDAKEDHASVLALSGQVKTQYAGPGGIQEIDQDAFFRPIT
                     VFNNTVADPTTAVKLLTRALRYAIIARGVAQLSLPNDIQREPLDATYCERETCLPEVG
                     VAPTNEAIAAAAEAIDGAARPVILAGFGAMRAAGPVLDLAETIQAPIVTTFRAKGILP
                     DENEWVAGLHGQLGTPHALTLVLKSDLVIACGASFSDLTGIPPEKPAVHIDIDPLQLG
                     KHPLAVGVWGDCAIAIPRIRERVRPRDDSGTRQWLAKKKQEWRDRLDREADPAAVPIR
                     PPYIMKVLSETLPEDAVISLDVGENQWWFGRNFRMKRQRFAMSGYLGTMGFGLPGAIA
                     AKLAYPEKNVVCITGDGGFGMVMADFTTAVKYDLPITVLVLNNHDLAMIREEQREADY
                     PSFGTDLTNPDFAAYAEACGGVGIRVKKPEDLTGAVLKALAMNKPALIDIETDPKRFA
                     "
     gene            complement(2372..3115)
                     /locus_tag="PLI76_09585"
     CDS             complement(2372..3115)
                     /locus_tag="PLI76_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HOI62325.1"
                     /translation="MTTSLGKRCIAEAVGTFILVFFGTGAAVVTLMLAAGTTPATPFN
                     IGIGALGGLGDWLAIGLAFGIAIAGAIYAFGRISGCHINPAVTIALFATRRFSGRDTV
                     AYIVVQLIGAAAASLLLAWVIGPDAVTIGGLGATTPFPGIGYLQAIVIEIIGTFLLML
                     VIMGVAVDERATPGFAGLMVGLAVAGIITTIGNLTGASLNPARTFGPYLGDWLLGGSN
                     LWAFFPIYIIGPIVGALLAAFLYDYLSGE"
     gene            complement(3179..3394)
                     /locus_tag="PLI76_09590"
     CDS             complement(3179..3394)
                     /locus_tag="PLI76_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2180 family protein"
                     /protein_id="HOI62326.1"
                     /translation="MKCYYCALEGKESEAVAVCIVCGMGLCMDHAIRKDIDVWEGGYP
                     LPSRRVKAPLPRILCPECYAALYGKEK"
     gene            complement(3398..4876)
                     /locus_tag="PLI76_09595"
     CDS             complement(3398..4876)
                     /locus_tag="PLI76_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2193 domain-containing protein"
                     /protein_id="HOI62327.1"
                     /translation="MINEAMAAQRADVDTVKAKRGQKFVIEDTKPYVDVAKKMTAIGN
                     QSQAVIDLHKDSVVSHYEVLKGLTTTVRPEDDPFVEHYQTPVVLEVLKEQDEAFAKSV
                     DTFVQKIADEEALIGLEAVRHYGGFYGPTCVVDFALIPGSTSNVVNQVLQKTDIPVEH
                     KRAILAAKSWAMNTSYGFGDHFAHALEAGATPTEAMAKEIATMQKIYREPVEAQAELM
                     DTAGMTSFDQRKYMSEYRRRMEGTIKAAMDDGVHYGNILTVPAYSVGDIAHHIAQTTF
                     NMCKDDVVMATIEAVTDVMESSLRRSLDSYKSYWDVLSVATGSSAAATEYLLQLDGFN
                     APMIVDLLTKRFHNYVQIYPSRGAAAELHNCDFMDMIYRGWKLLDKAERMRNGSDEAL
                     VPMVGKYPVDLSPIHKNEVLMNPQWYVYAACAISVRFSALMRLSDYPCLLTSEPVTAT
                     MMTNIIALEKETAAAPVRACKNCASACLVDMRHQYCQWKEAV"
BASE COUNT          900 a         1484 c         1600 g          894 t
ORIGIN      
        1 cggtgggccg gtcgagcagg atgcccatgt ggcttgcgat gccgaggccg cggggatggg
       61 cgatcccctg tccgtcgaag aggatgacgt ccggctcgga ccgcagtctc cggaacgcct
      121 caaggagggc gggaccttcc cggaaggtga gaagtcccgg tatgtaggga aacgacgtct
      181 cgatagaggc agagacacgc tcgacgacgg cgaggccggg ataccggaga gcaacggcga
      241 ccgcatggat ctcgtttgag cctctcgcgt aggaggcgtc ggcgccggcg aggagggtga
      301 cctcaccgat atcgccggag aggctgatcc tcgtccggag cgtctcctgg aggcgaacgg
      361 cctccgccgg ggcaaggttg aacgggtggg tctcgtcgat ctccatgatc accgataccc
      421 tgatccttac agactattac tgcctgttgg tgggtgtggc ccgaaccggt tgccggggtt
      481 cgggggcatt tttatatgta gtgaggtagg gtacggctcg cgagtcatct ccgggacgtg
      541 agcggtatgg cgcgatgggt atgcagcgtc tgcgattacg agtacaacga ggaggcaggg
      601 gaccctgaca tcggtatacc cccgggcaca ctgtttgagg atctccccgc tgattggcgg
      661 tgtccccgtt gcaccgtggg aaagggagcg tttgtgcggg ctgatgaaga ggaagaggtg
      721 aagactgatg aagaagacga cctttgaagg ggctatagcg acggtggccg atgtaatggt
      781 agcagagctt gaagcatggg gggtcaacct ctacttcggt atgcccggag cgtcggcgct
      841 cccgctcgtg gacgcggtcc ggaggaacag gaacgcccgg tacatcgtcg tccggcacga
      901 gcagaccgcg gccctcgcgg cgtccgccta caacaagttc acgggaaaga tagcggtctg
      961 cctgaccatc gccgggccgg gagcgacgaa ccttgccacc ggcctatacg acgcaaagga
     1021 ggaccatgcg agcgttctcg cgctctccgg gcaggtgaag acgcagtacg ccggccccgg
     1081 cggcatccag gagatcgacc aggacgcctt cttccggccc atcacggtct tcaacaacac
     1141 cgtcgccgat ccgacgacag cggtcaaact cctcacccgg gcgttgcgct acgctatcat
     1201 cgcccgcggc gtcgcccagc tctcgctccc gaacgatatc cagagagaac cgcttgatgc
     1261 aacttactgc gagcgtgaga cctgcctccc ggaggtcggg gttgcgccga ccaatgaggc
     1321 catcgcggcc gcggcggagg cgatcgacgg cgccgcacga ccggtcatcc ttgcgggatt
     1381 cggggccatg cgtgcggccg gtccggtcct cgacctcgcg gagacgatcc aggcgccgat
     1441 cgtcaccacc ttccgggcga agggcatcct ccccgacgag aacgagtggg ttgccgggct
     1501 ccacgggcag ctcgggacgc cgcatgccct gacactcgtc ctgaaatcgg acctggtgat
     1561 cgcctgcggg gcgagtttct ccgacctcac cgggatcccg ccggagaaac cggccgtcca
     1621 tatcgatatc gacccgctcc agctcgggaa gcacccgctt gcggtcgggg tctgggggga
     1681 ctgcgccatt gccatacccc ggatccgtga gcgggtccgg cctcgggatg actccgggac
     1741 ccggcagtgg ctcgcgaaga agaagcagga atggcgcgac cggctcgacc gggaagccga
     1801 cccggctgcc gttcccattc gcccgcccta catcatgaag gtcctctcag agaccctgcc
     1861 ggaggatgcg gtcatatcgc ttgacgtcgg ggagaaccag tggtggttcg ggcggaactt
     1921 ccggatgaag cggcagcggt tcgcgatgtc gggctacctc gggacgatgg ggttcgggct
     1981 gcccggggcg attgcggcga aactggccta tccggagaag aacgtcgtct gcatcaccgg
     2041 ggacggcgga tttggcatgg tcatggccga cttcaccacg gcggtaaaat acgaccttcc
     2101 catcacggtc ctcgtcttga acaaccacga cctcgccatg atccgcgaag agcagcggga
     2161 agcggactac ccctccttcg gcaccgacct cacgaacccg gactttgcgg cgtatgcgga
     2221 ggcctgcggc ggcgtcggga tacgggtgaa aaagccggag gatctcaccg gggctgttct
     2281 caaggcgctg gcgatgaata agcccgccct catcgatatc gagaccgacc ctaagcgctt
     2341 tgcgtgagga aaaaaaaggg aaggggccgg ctcattcgcc ggagaggtag tcgtagagga
     2401 acgcagcgag caacgcaccg acgatgggcc cgatgatgta gatggggaag aatgcccaga
     2461 ggttcgaccc gccaagcagc cagtccccga ggtacggccc gaacgtacgg gcagggttca
     2521 acgacgcgcc ggtcaggttc ccgatcgtcg tgatgatccc cgcgaccgcg aggcccacca
     2581 taagcccggc aaagcccggt gtggcccgtt cgtcgacggc gacgcccatg atgacgagca
     2641 tcagaaggaa cgtcccgatg atctcgatga cgatcgcctg caggtagccg atgccgggga
     2701 agggcgttgt tgccccgagg cccccgatgg tgacggcgtc cgggccgatg acccaggcta
     2761 agagcaggct tgcggccgca gcgccgatga gttgcacgac gatgtaggcg accgtatcgc
     2821 gcccggagaa ccgccgggtc gcaaagaggg ctatcgtcac cgccgggttg atgtggcagc
     2881 ctgatatcct tccgaaagcg tagatcgccc ctgcgatggc gataccgaag gcaagaccta
     2941 tagcgagcca gtcgccgagg cccccgagcg ccccgatccc tatgttgaag ggggtcgccg
     3001 gggtggtccc cgcggcgagc atcagcgtga ctactgcggc gccggtaccg aagaagacca
     3061 ggatgaatgt cccgacggct tcggcgatgc atcgttttcc aagggatgtg gtcatgatta
     3121 caccagattg gcatagattt tacgagaagc cgaccaaaaa aggtcggggg cgtgtgcttt
     3181 acttctcctt tccatacagg gcagcatagc actcgggaca gaggatcctc ggcagtggag
     3241 ccttcacgcg cctgctgggg agcgggtagc cgccctccca gacatcgata tcctttctga
     3301 tggcatgatc catgcagagt cccatcccgc agacgatgca gactgccact gcttcgctct
     3361 ccttcccctc aagggcgcag tagtagcact tcatggttta gaccgcctct ttccactggc
     3421 agtactggtg ccgcatgtcg accaggcacg ccgaggcaca gttcttgcag gcccgcaccg
     3481 gggcggctgc cgtctccttc tcgagagcga tgatgttcgt catcatcgtc gcggtcaccg
     3541 gctcgctcgt caggaggcac gggtaatccg aaagccgcat aagggcggag aaccggacgg
     3601 atatcgcaca ggccgcgtag acataccact gcgggttcat cagcacctcg ttcttgtgga
     3661 tgggcgagag gtcgacgggg tacttgccga ccatgggaac cagcgcctca tcggagccgt
     3721 tgcgcatccg ctctgccttg tcgaggagtt tccacccgcg gtagatcatg tccatgaagt
     3781 cgcagttgtg gagttctgcc gccgctcccc gggacgggta gatctggacg tagttgtgga
     3841 accgcttcgt gagcaggtcg acgatcatgg gggcgttgaa cccgtcgagc tggaggagat
     3901 attcggttgc ggcggcagac gacccggtcg cgaccgagag gacgtcccag taactcttgt
     3961 aagagtcgag cgatctccgg agcgaggact ccatcacgtc ggtgaccgcc tcgatcgtcg
     4021 ccatgacgac gtcgtccttg cacatgttga aggtcgtctg cgcgatgtgg tgcgcgatgt
     4081 cgccgacgct gtaggccggg acggtcagga tgttgccgta gtggacaccg tcatccatcg
     4141 ccgccttgat cgttccttcc atgcggcgcc ggtactcgct catgtacttc cgctgatcga
     4201 aggaggtcat cccggccgta tccatcagtt ccgcctgggc ttcgaccggt tcgcggtaga
     4261 tcttctgcat ggtcgcgatc tcttttgcca tcgcctcggt gggggtggct cctgcctcaa
     4321 gggcgtgggc gaagtggtct ccaaagccat aggaggtgtt catggcccat gatttggcgg
     4381 cgaggatcgc tcgcttgtgc tcgaccggga tatcggtctt ctgcagcacc tggttcacca
     4441 cgttgctcgt gctccccggg atcagcgcaa agtcgacgac acaggtcgga ccgtagaacc
     4501 cgccgtagtg ccgcaccgcc tcaaggccga tcagagcctc ctcatccgcg atcttctgga
     4561 cgaaggtatc gacgctcttc gcaaacgcct cgtcctgctc cttcaggact tcgagcacca
     4621 cgggtgtctg gtagtgttcg acgaacgggt cgtcttcggg gcggacggtc gtagtaaggc
     4681 ctttcaagac ctcgtagtgc gagaccaccg agtccttgtg gaggtcgatg accgcctggc
     4741 tctggttccc gatcgccgtc atcttcttcg ccacgtcgac gtagggtttc gtgtcctcga
     4801 tgacgaattt ctggccgcgt ttcgccttta ccgtatccac gtccgcccgc tgggcggcca
     4861 tggcctcgtt tatcatct
//
LOCUS       DAOSEE010000147         4771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_6313_length_4771_cov_207.181086, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000147 DAOSEE010000000
VERSION     DAOSEE010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6313_length_4771_cov_207.181086"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="PLI76_09600"
     CDS             <1..1128
                     /locus_tag="PLI76_09600"
                     /inference="COORDINATES: protein motif:HMM:TIGR04275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62328.1"
                     /translation="GPAGATAITSGPGDNEFPAVSGNTIVWFDNTTKSVKVYDVASGR
                     SLTIPGNNTYALVSMRQVDVSENHVVWAGMSAATMTSDIYLYDIASGSVTPLTSGQGY
                     QMFPAIMDNLIFWDQIDMATGKGEIYWYNIANGHTGSLLKNSSINQLFPAAGGGFVTW
                     LDDGKVSGRLDFRWSSLQTGNITRLISTSSVISPPTVSSDGRWIVWTAMDEAGNAPYL
                     RMLDTTTKKIQKITGEGAMPANPAVDGDFIVWTDYRNGNGDIYLYDIQSGQERQLTSE
                     RAEEAFPDISDGRIVWMGNNDGYWNIYSATVGGRAQPTVAPTGFPTVAPTGFPTVAPT
                     GFPTFAPTGFPTPAPTGFPTFAPTGHHTEEPHHTEEPHHFT"
     gene            complement(1346..2662)
                     /locus_tag="PLI76_09605"
     CDS             complement(1346..2662)
                     /locus_tag="PLI76_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOI62329.1"
                     /translation="MNESNDVFIARGPVLIAGATINGSTVDIAVDETGTIAGIGEGIR
                     REHDVDVIIDGSDRLAFPGLVNTHTHAAMTLLRGYGDDMPLQEWLSQKIWPLEAHLTG
                     DDVYAGTRLACLEMIRSGTIAFNDMYFFMDRAAQAVDDMGIRATLAYGFIDLGNAEKR
                     EAEIKATESLVTRIKSLANPRIRAAVGPHSVYTVSPEGLSWCADFAREQEIGIHVHLS
                     ETEKEVVDCVAQFGKRPAAHLDDCGCLTPRTVAAHCCWLDEAECRLLGKRGVSASHNP
                     ASNMKLAVNRAMPYHWLKQHGANVALGTDGCASNNNLDLMEEMKFAALLQKFAWNSPT
                     LLSAGEAVAMATTAGARALGTGPGTLTVGAPADIILLDARAVCNTPLHHADSNAVYAC
                     NGGAVMTVLCQGRVLMHEREVAGEEEIVREAAAAAQALVGRAREAA"
     gene            complement(2659..3330)
                     /locus_tag="PLI76_09610"
     CDS             complement(2659..3330)
                     /locus_tag="PLI76_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HOI62330.1"
                     /translation="MLGIIGGTSLLFADLPPLEETTVATPYGRAEVRTGAFALLMRHQ
                     HNLPPHRINYRACLAALAILGVDTVIAFGSAGSLRPDIPPGSIVIPTDYLSATDIPSI
                     HECTIGHICPRLDADLIRSLGELIPEARVGGVYAQTRGPRLETVAEVRALACAADLVG
                     MTVASEATLAMELDMRFAAICTVDNYANGLGETALTFDQVLETSRANARRMEKILENI
                     VERYT"
     gene            3512..4636
                     /locus_tag="PLI76_09615"
     CDS             3512..4636
                     /locus_tag="PLI76_09615"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOI62331.1"
                     /translation="MGRFLMVGEDAIRAGRCTDVYFRRVVDVMEKDGVNPRVTMEVTA
                     TALPDPWGVFCGLDDVLRLLEGIPVDVDAMPEGSIFSANEPVLRISGRYRDFAVYETA
                     ILGFLCHASGVASAAAHVRLAAGGRPVYSFGSRRQHPAIAAMIERAAWIGGVDAASNT
                     CAPEGIALAGTMPHAFVMCYPRPEDAWSAFAREAAPEVPRIMLCDTFSDERAEAVRAA
                     ACGATAVRLDTPRSRRGDMRAIIEEVRWELDVHGYPDVKIFLSGGLSREDVAAYRDVA
                     DAFGVGGAIANAPVIDFAMDIVEMEGKPYAKRGRRSGAKQVYEMAGRRVTLPLEAPAP
                     EGGVGLLSRFIEGGAIRTRPKMEDARERVLSRLSGLARRG"
     gene            4695..4766
                     /locus_tag="PLI76_09620"
     tRNA            4695..4766
                     /locus_tag="PLI76_09620"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4727..4729,aa:Ala,seq:ggc)
BASE COUNT          805 a         1452 c         1647 g          867 t
ORIGIN      
        1 ggaccggccg gggcgaccgc gatcacgagc gggccggggg acaacgagtt tcctgcggtc
       61 tcagggaata ccatcgtctg gttcgacaac accactaaat cggtcaaggt ctatgatgtt
      121 gcatccggac ggagcctgac gattccgggg aacaacacct atgcgctggt atccatgcgg
      181 caggtggatg tctcagagaa ccacgttgtc tgggccggca tgagcgccgc gacgatgacg
      241 tctgatatct acctttacga tattgcaagt ggttcggtga caccgctcac gagtggccag
      301 ggctaccaga tgtttccagc gataatggat aacctcatct tctgggacca gatcgatatg
      361 gcgaccggga agggcgagat ctactggtat aacattgcta acgggcacac cggttcgctt
      421 cttaaaaatt catcgattaa ccagctcttc ccggccgctg gcgggggttt cgtaacatgg
      481 ctcgatgatg ggaaagtcag tggtcgcctc gattttcgct ggtcttcact ccagaccggg
      541 aatataacac gcttgatctc gacgagcagt gtaatcagtc cccccacggt atcgagcgat
      601 gggagatgga tcgtctggac tgctatggat gaggctggga atgctcccta cctgcgcatg
      661 ctggatacca ccaccaagaa gatacagaag atcaccggtg agggagccat gccggccaat
      721 cccgccgtcg atggcgactt tatcgtctgg accgattacc ggaacggaaa cggcgatatc
      781 tatctctacg atatccagag cgggcaggag agacagctca catccgagag agcagaagag
      841 gcgttcccgg atatatccga tggcaggata gtctggatgg gcaacaacga cggttattgg
      901 aacatatact ctgccaccgt cggcgggaga gcacagccga ccgttgcgcc taccgggttc
      961 ccgaccgttg cacctaccgg gttcccgacc gttgcaccta ccgggttccc gacctttgcg
     1021 ccgaccgggt tcccgacccc cgcaccgacg gggttcccga cctttgcacc gaccggacac
     1081 cacactgagg aaccgcatca caccgaggaa ccgcaccact tcacctgatg gggagagcgc
     1141 atcgtccctg gcggggcgat ctcccgcgcc tttgcaggcg gtacgccggt cctcgtcagg
     1201 tttagcggtg acggggacaa cgtactgccg ctctccagga gcgatgtgcc ctgcccggat
     1261 cgtgggcggt cccggtgcgc cgggagaaaa gacagccggc gttccgggcc atacgagggg
     1321 ctgcccggaa caacccccgt gcggatcagg cagcctcgcg agcccgcccg accagcgcct
     1381 gcgccgccgc cgcggcctcc cggacgatct cttcctcgcc tgccacctcc cgctcgtgca
     1441 tgagaacccg gccctggcag aggacggtca tgaccgcgcc gccgttgcag gcgtagacgg
     1501 cgtttgaatc ggcgtgatgg aggggggtgt tgcagaccgc ccgggcgtcg aggaggatga
     1561 tatcggccgg cgcaccgacg gtcagggtcc ccgggccggt gccgagcgcc cgggcgcccg
     1621 cagtcgtcgc catcgcgacc gcctcgcccg cggaaagcag ggtcggcgag ttccaggcaa
     1681 acttctgcag gagggcggca aacttcatct cctccatcag atcgaggttg ttgttcgagg
     1741 cacacccatc ggtcccgagg gcgacgttcg ccccgtgctg cttcagccag tggtagggca
     1801 tggcccggtt gacggcaagc ttcatgttgc tcgccgggtt gtgggatgcc gagacgccgc
     1861 gcttgccgag gagccggcac tcggcctcgt cgagccagca gcagtgcgcg gcgacggtcc
     1921 ggggggtgag gcacccgcag tcgtcaaggt gcgccgcggg gcgtttgccg aactgtgcga
     1981 cgcagtcgac gacctccttc tcggtctcgg agagatggac gtggatgccg atctcctgtt
     2041 cccgtgcaaa atcggcgcac caggaaagac cctccggcga gacggtgtag accgagtgcg
     2101 ggccgacggc tgcccggatc cgcgggtttg caagcgattt tatgcgggtg acgagggatt
     2161 ccgtcgcctt gatctcggcc tcccgcttct cggcgttccc gagatcgatg aacccgtagg
     2221 caagtgtcgc ccggataccc atatcgtcga ccgcctgcgc cgcccggtcc atgaagaagt
     2281 acatatcgtt gaacgcgatc gtgccgctcc ggatcatctc aaggcaggcg agcctcgtgc
     2341 cggcgtagac gtcgtcgccg gtaaggtggg cctcaagcgg ccagatcttc tgcgagagcc
     2401 actcctgtag cggcatgtcg tccccgtagc cccggagcag ggtcatcgca gcgtgggtgt
     2461 gggtgttgac gaggccgggg aaggcgagcc ggtcggaacc gtcgatgatg acgtcgacgt
     2521 catgctcgcg tcggatcccc tccccgatcc cggcgatcgt tcccgtctcg tcgacggcga
     2581 tgtccacggt cgatccgttg atggtggccc cggcgatcag caccggtccc cgtgcgataa
     2641 atacgtcatt tgactcgttc atgtgtatct ctccacgata ttctccagga tcttctccat
     2701 ccttctggca ttcgcccgcg aggtctcgag cacctggtca aaggtcaggg ctgtctcccc
     2761 gaggccgttt gcgtagttgt ccacggtaca tatggcggca aaccgcatat cgagttccat
     2821 ggcgagtgtc gcctcgcttg caaccgtcat cccgacgagg tccgctgcgc aggcgagcgc
     2881 tcttacctcg gcgaccgtct cgaggcgcgg cccccgggtc tgggcgtaga ccccgcccac
     2941 ccgggcctcc gggatgagtt cgccgaggct gcggatgagg tccgcgtcca gccgggggca
     3001 gatgtggccg atggtgcact cgtggatgga cggtatatcg gtggcgctga ggtaatcggt
     3061 ggggatgacg atcgagcccg gcggtatatc cggtctcagg gagccggcgg aaccgaacgc
     3121 gatgaccgtg tccacgccga gaatggcgag cgcggcaaga cacgcccggt agttgatgcg
     3181 gtgcggcggg aggttgtgct ggtggcgcat gaggagcgcg aaggctccgg tgcggacctc
     3241 ggccctgccg tagggggtcg cgacggtcgt ctcctcgagc ggcgggaggt cggcgaagag
     3301 gaggctcgtg cctccgatga tcccgagcac tatacgcacc ccgcacggtg gtgcaggccg
     3361 tcccggcggg ccgggagagc cgtagcggca tacgctcgtc cgtgtgacat gctatgtaga
     3421 gggttggcgc ccggatggga aaattccatc tatacggtgt tgcggaatcg gttcgcaaga
     3481 gtttacccct ccggcgacga ccggatcagg catgggcagg tttctgatgg tcggcgagga
     3541 cgccatccgg gccgggcggt gcacggacgt ctacttccgg cgcgtcgtgg acgtcatgga
     3601 gaaggacggg gtcaatcccc gcgtcacgat ggaggtgacg gccacggcgc tcccggaccc
     3661 gtggggggtc ttctgcggcc ttgacgacgt tctccggctg cttgagggca tcccggtgga
     3721 tgtggatgcg atgccggagg gctcgatctt ctctgcaaac gaaccggtcc tccgtatatc
     3781 ggggcgctac cgggattttg cggtctacga gacggcgata ctcgggtttc tctgccacgc
     3841 gtcgggggtg gcgtcggcgg cggctcatgt ccggcttgct gccgggggcc ggccggtcta
     3901 ctcgttcggc tctcgccggc agcacccggc gatcgcggcg atgatcgagc gggcggcctg
     3961 gatcggcggg gtggacgccg cgagcaacac ctgtgcgccg gagggaatag cgctcgcggg
     4021 gacgatgccc cacgcgtttg ttatgtgcta tccacggccc gaggatgcct ggtctgcgtt
     4081 tgcgcgggag gctgccccgg aggtgccgcg gatcatgctc tgcgacacct tctccgacga
     4141 gcgggccgag gcggtcaggg cggcggcatg tggggcgacg gcggtgcggc tcgacacgcc
     4201 gcggtcccgg cggggggata tgcgggcgat catcgaggag gtgcgctggg agctcgacgt
     4261 ccacggctac ccggacgtga agatcttcct ctcgggcggg ctctcgcgcg aggacgttgc
     4321 ggcataccgt gacgtcgcgg acgccttcgg cgtcgggggg gcgatcgcga acgcgccggt
     4381 gatcgatttt gccatggata tcgtcgagat ggaggggaaa ccctacgcga agcgcgggag
     4441 acggagcggc gcaaagcagg tctacgagat ggccggccgg cgggtcacgc tgccccttga
     4501 ggctcctgcg ccggagggcg gggtggggct cctctcccgg ttcatcgagg gcggtgctat
     4561 tcgcacacgc ccgaagatgg aggatgcgcg ggagcgggtg ctctcccggc tttccggcct
     4621 tgctcgccgg ggatagggga gcggccggcc acaacccttt ttatgggatc acgatcaaca
     4681 tagtagggta acaggggccg atagatcagg gggagattgc ttccttggca tggaagaggc
     4741 cgcgggttca attcccgctc ggtccatgtt t
//
LOCUS       DAOSEE010000148         4728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_6390_length_4728_cov_218.333191, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000148 DAOSEE010000000
VERSION     DAOSEE010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6390_length_4728_cov_218.333191"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2691
                     /locus_tag="PLI76_09625"
     CDS             <1..2691
                     /locus_tag="PLI76_09625"
                     /inference="COORDINATES: protein motif:HMM:NF023252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3344 domain-containing protein"
                     /protein_id="HOI62332.1"
                     /translation="DDSGGGEAVDYGYTGKRLDIAASGTLHGNLTYYDFGSYTGLIPA
                     GESREFAIDIPAPANGTIRYARLYVYTTWGHDEKKKEGTTVRIDLSVDGRAIRADRTY
                     NDRKYEGIYNYLIETHAFNVTGIPGGIAGEHVVKVKNDGRKDDLFAAYGVSLLVVTED
                     PDEPETTYWIAEGSDAVFASSQVGTTSEDCTTTAVFEDPIDPATTGEARLILVSTAGS
                     GMGNDEHRILFNDGEWFNLLAAGSSAVSVADLDARPFLRQSANEAGIQSLITATKGDY
                     MENRAIILVVRKGSPPEGYEMNATPTTSGPGTPAVSADTGSRIVLDAANGRIGAMHIT
                     ADGGAVDLYIPEGAAIVDGAKDPVSVLVLQRLRPGDAGGVERAYAVGSDDMASDIALT
                     LTISADAPGGVIARYDRSSGAWQPLETRADAAGGTVSAQIRQGGTYAVRHDGHAPAAH
                     ALSGGLLNTLANIPAGGLSGRLLEAVTAFLGLFGIVPAGEVPAMTAAPVPAPTPGSVP
                     AALEPAPAPDITAMRFALSVLSDPPGALVDIDGEYTGRATPAAFASLPGGNHTVRVHA
                     DGAEPVEETILLDHDDEVFIEFPGAGTSPAGPLRTWEQNHYGGVYVDSFPAGADILVD
                     GQKVNRKTPDIVYGLKEGLHTIKLRKENTAFSSEKEQVWVERNTITRVTFASGNADLV
                     RSITFDSEECAQKPFSVNGRYLGDRLPKKVDVRGIGGAYITVRDSESYRSYRIPATAG
                     SNDTVTVTLPDAPCSLLVTSVPAGAAIAVDGFLTGFATPYVIENISEGKHRIAVSKPG
                     HIPAEREVLLTDGSGDTTVKVTLEPYVWGSLAVSSTPSDAKIYLFGRDTGEVTPHTFH
                     YLDIGSYPVKVVGKEGSKTIEGVLVSPHAVAGCHADLTGE"
     gene            2881..>4728
                     /locus_tag="PLI76_09630"
     CDS             2881..>4728
                     /locus_tag="PLI76_09630"
                     /inference="COORDINATES: protein motif:HMM:NF023252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3344 domain-containing protein"
                     /protein_id="HOI62333.1"
                     /translation="MHDTTSQGGCRSPRLFFFIAVGLLAAALLPAAVSADPHVGDILF
                     DADMADDATQGPASASFAPGARIVAATADCDLTIGGMPNPTGSVVFAREPNTIRVMNI
                     KNNGPGTSPATVMEVRSDDGWTGRVDIPPIESGKNILSVSIEDPTIRNLAGGTVTYTA
                     EIDPDDTVAESDESNNIRVGQPREVKYNGYKGKRYWEGGSDITTVRTYDLYGGIVHSF
                     GDSRYWSGSFGGDDYWKGYTVTWTADDLPLPEGSSVRDAWLYVPYAWDNTNSAPDNVS
                     IEFNGVRIPYVNWYTDVSNFGAYVDHHYGLFTYNVTSLFRKGANNTALFERDRPGKIS
                     PAGFTLAVVYEDPSATRKQIFINEEFDILGADPLGYGTNETEATAYVPFTGMTIDTGN
                     VVRADLTTFVPWGDCGDGTRPGEGNLLFNDELVREWVWDYGPGTSTQVAVDERDVGDR
                     LRPTGNIAAIQGTEANLPTMVAAQVFLVVEYVDDSSGDAPVAAFTAAPMSGLAPLAVT
                     FADASTNSPTAWSWESRPSGTTENWTLFSTDQSPTHTFAAGAYDVRLTATNAGGSDTL
                     TKARCISSSAGPKRLATVQSGTVSGDLYVGAYGGWSSGISYATNTFNRTF"
BASE COUNT          919 a         1507 c         1513 g          789 t
ORIGIN      
        1 gacgattccg ggggcgggga agccgttgac tacggttaca cggggaagag gctggatatc
       61 gccgcctcgg gcacgctcca cgggaacctg acctactacg atttcggcag ttacacgggg
      121 ctcatccctg ccggcgagag cagggagttt gcgatcgata tccccgcccc tgcgaacggc
      181 acgatccggt acgcacggct gtacgtctac accacatggg ggcacgacga gaagaagaaa
      241 gaagggacca cggtcaggat cgatctctcg gtcgacggga gggcgatcag ggcggacaga
      301 acctataacg accgtaaata cgaggggata tacaactacc tcatcgagac ccacgcgttc
      361 aacgtcaccg gcatcccggg cggcatcgcc ggagaacacg tcgtgaaggt gaagaacgac
      421 gggagaaagg acgatctctt tgcggcgtac ggcgtcagcc tcctggtcgt caccgaagac
      481 ccagacgaac ccgagacgac ctactggatc gcggaggggt cggatgcggt ctttgccagt
      541 tcgcaggtcg ggacgacctc tgaggactgt acaacgacgg cggtctttga ggacccgatc
      601 gatcctgcca ctaccggcga agcccgtctc atcctcgtat ccacggccgg ttcgggtatg
      661 ggcaacgacg agcaccggat cctcttcaac gacggcgagt ggttcaacct gcttgccgcc
      721 ggttcgtcgg cggtcagcgt ggccgacctg gatgcgagac cgttcctccg gcagtcggca
      781 aacgaggcgg gtatccagag cctcatcacc gcgacgaaag gagactacat ggagaaccgc
      841 gccatcatcc tcgtcgtcag gaagggcagt ccgccggagg gctacgagat gaacgccacg
      901 cccacgacga gcggacccgg aactccggcg gtatctgccg ataccggatc gcggatcgtc
      961 cttgacgcgg caaacgggcg gatcggggcc atgcacatca ccgccgacgg cggggccgtc
     1021 gacctctaca tccccgaggg agcggcgatc gtcgatgggg ccaaagatcc tgtttcggtg
     1081 ctggtgctgc agaggctcag gcccggggat gccggaggtg tcgagcgggc ttacgctgtc
     1141 ggcagcgatg atatggcgag cgatatcgcc ctcacgctga ccatatccgc ggatgcaccg
     1201 ggaggggtga tcgccaggta cgaccgctct tcgggcgcct ggcagcccct ggagacgagg
     1261 gccgatgcgg cgggaggcac cgtctctgcg cagatacggc agggagggac ctatgctgtc
     1321 cggcacgatg ggcacgctcc ggcagcgcat gctctctccg gcgggctcct caataccctc
     1381 gcgaacatcc ccgccggggg actctccggc aggctgctcg aggcggtcac ggcgttcctg
     1441 gggctctttg gcatcgtccc tgccggggag gtgccggcga tgacggcagc ccctgtgccg
     1501 gcacccaccc ccggttcggt gccggcagcc cttgagccgg cgccggcacc cgatatcacc
     1561 gctatgcggt ttgctctctc ggtgctctcc gatccgccgg gcgcgctcgt cgatatcgac
     1621 ggggagtata ccggcagggc gacaccggca gcatttgcgt cgctcccggg cggcaaccat
     1681 accgtccggg tgcacgcaga cggcgcagaa ccggtggaag agacgatcct gctcgatcac
     1741 gacgatgaag tcttcatcga gttcccggga gccgggacat cccctgcggg accgctccgc
     1801 acctgggagc agaaccacta cggcggcgtc tacgtcgact cgttcccggc gggggcggat
     1861 atcctcgtcg acggccagaa ggtgaaccgg aagactcccg atatcgtcta cgggctcaag
     1921 gaggggttgc acacgatcaa actgcgcaag gagaacaccg cgttcagctc cgagaaagag
     1981 caggtctggg tcgaaagaaa caccatcacc cgggtgacgt ttgcgagcgg caacgcggac
     2041 ctggtgcgct cgatcacgtt cgactcagag gagtgcgcgc agaaaccctt ctccgtcaac
     2101 ggcaggtacc ttggcgaccg tctcccaaag aaggtcgacg tcagggggat cgggggggcg
     2161 tatatcaccg tccgcgacag cgagagttac cggagttacc ggatacccgc caccgccggg
     2221 tcaaacgaca ccgtgaccgt caccctcccc gatgcaccct gcagcctcct cgtgacgtcg
     2281 gtgccggcgg gagcggcgat agcggtcgac ggatttttga cagggtttgc gacgccatac
     2341 gtcatcgaga acatatctga gggcaaacac cggatagcgg tctcaaaacc cgggcacata
     2401 ccggctgagc gggaggttct gctgacggac ggttccggcg ataccacggt gaaagtcacc
     2461 ctcgagcctt acgtctgggg gtcgcttgcg gtctcgagca caccgtcgga cgcgaagatc
     2521 tacctcttcg ggcgggacac cggcgaagtg acgccccaca ccttccacta cctcgatata
     2581 ggcagttacc cggtgaaagt ggtggggaag gaggggtcga agacgatcga gggcgtgctg
     2641 gtctcgccgc atgccgtcgc cgggtgccac gcggacctta cgggggagtg aaagttctcc
     2701 cttttttata acctcccctc tccgcagccg gatacgcacc atggccgata tacgtgtcct
     2761 gataaccggg aaaaaccttc tcgcggggcg gattcccgga gtgattacaa atattttata
     2821 taatgttact tgatcatata ctctcattcg attgagggat caagtgtgat taactttaag
     2881 ttgcatgata ctacatccca gggcggctgc cggtcgcccc ggttgttctt cttcatcgcc
     2941 gttgggctcc tggcagcggc gctgctcccg gcggcggtat ccgccgatcc gcacgtaggc
     3001 gatattctct ttgatgccga tatggcggac gacgccacgc aggggccggc ctccgcctct
     3061 ttcgcgcccg gcgcccgcat cgtggccgct acggcagact gcgacctcac catcgggggg
     3121 atgccaaacc ccaccggcag cgtggtgttt gcccgcgagc ccaacaccat ccgggtcatg
     3181 aacatcaaga acaacggccc gggaacctcc ccggcaacgg tgatggaggt ccgctcagac
     3241 gacgggtgga cgggcagggt cgacataccg ccgatcgagt ccggcaagaa catcttgagc
     3301 gtctcgatcg aggatccgac catccggaac cttgctggcg ggacggtgac ctacaccgcc
     3361 gagatcgatc ccgacgacac cgtcgccgag agcgatgagt ctaacaacat cagggtcggc
     3421 cagcccagag aggtgaagta caacggttac aagggcaaac gctactggga gggcggcagc
     3481 gacatcacca ccgtccggac ctacgacctt tatggcggca tcgtgcactc cttcggcgac
     3541 agccgttact ggtcggggag tttcggcggc gacgactact ggaagggtta cacggtgacc
     3601 tggacggcgg acgacctgcc gctgcctgag ggctcgagcg tccgcgacgc ctggctctac
     3661 gtgccctacg cctgggacaa caccaactct gcacccgaca acgtctcgat cgagttcaac
     3721 ggcgtccgga tcccctacgt gaactggtac accgatgtct cgaacttcgg ggcgtatgtc
     3781 gaccatcact acgggctgtt cacctacaac gtgacgtcgc tcttccggaa gggcgccaac
     3841 aacaccgcgc tctttgagcg ggacaggccg ggcaagatat cgccggcagg gttcacgctt
     3901 gcggtggtct acgaggaccc ctccgccacc cgaaagcaga tcttcatcaa cgaggagttc
     3961 gacatcctcg gcgccgatcc gctcggttac ggcacgaacg agaccgaggc tacggcgtac
     4021 gtgccgttta cggggatgac gatcgatacc gggaacgtcg tccgcgcaga cctcacgacg
     4081 tttgtcccct ggggggactg cggcgacggt acgagacccg gcgaggggaa cctcctcttc
     4141 aacgatgaac tggtccggga atgggtctgg gactacggcc cgggcacctc cacccaggtg
     4201 gccgtggatg agcgggacgt cggggaccgc ctccgcccga cggggaacat cgcggcgatc
     4261 caggggaccg aggccaattt gccgacgatg gtggcggcgc aggtcttcct ggtcgtcgaa
     4321 tacgtcgacg attccagcgg ggatgcgcct gttgccgcgt tcaccgcagc cccgatgagc
     4381 ggacttgcgc cgcttgccgt caccttcgcc gacgcctcga cgaactcccc caccgcatgg
     4441 tcgtgggagt cgcggccgtc cgggaccacc gagaactgga cgctcttctc caccgaccag
     4501 agcccgaccc acacctttgc agcgggagcc tacgacgtcc gtctcaccgc gaccaatgcc
     4561 ggcggcagcg atacgctcac gaaggcccgg tgcatctcgt cctctgcggg gccaaaacgc
     4621 cttgccacgg tccagagcgg cacggtctcc ggcgatctct acgtgggggc atacggggga
     4681 tggagctccg gtatctcata cgcgacgaac acattcaacc ggacattc
//
LOCUS       DAOSEE010000149         4718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_6405_length_4718_cov_240.633069, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000149 DAOSEE010000000
VERSION     DAOSEE010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4718
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6405_length_4718_cov_240.633069"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="PLI76_09635"
     CDS             complement(<1..507)
                     /locus_tag="PLI76_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HOI62334.1"
                     /translation="MFRPQDDFTYLMPVHFGGGKFDPGTLITQKATGLAVSYETERDL
                     LENYIPEGFELLAPEVQVAFNKFTEINWMHGGQYNLINVSAPVRFHGKKDELDGAYTL
                     VVWENKTAPILGGREQTGIPKIYADIEDLHIVRPHYATTVSYEGSTFLTMNFEAAGPI
                     TGRDLDTLK"
     gene            822..1454
                     /locus_tag="PLI76_09640"
     CDS             822..1454
                     /locus_tag="PLI76_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62335.1"
                     /translation="MGFTSQSGEKNNPVVRAESELSLEVVILIVFGVFMLLFGLLLFR
                     IQTGELPYAPDSTYGLFLVIVSFQVITMGKTPFGDLRRSWAVIVLGVCTAIVGMVACF
                     IPGLLAEPVRILVGILLSAGGVALLVQLFFSEDKARVWLKVPGALQHLTVACGLVYVV
                     SIVLGIITLLPGITTDTVTAVFLVIYGVGFFYLSWSIQKAGRLYPPGEAE"
     gene            1451..2065
                     /locus_tag="PLI76_09645"
     CDS             1451..2065
                     /locus_tag="PLI76_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62336.1"
                     /translation="MTPNASTATGGTGLFGEASLPLSIALIIPVGVLLVLLGLLLFPV
                     NQGLIPFSPDGQLGLLLVIMAIQMLALGETPVGQYRRSWLLIGIGIVFAALGIFSCIV
                     PGILTDAIRVLLAFLNIAGGAILLGKRFLPMLQGTGSAAELAHLPPIYKRLLGTLTVL
                     NVVAIAFGISMLLPGLVPGMVIAGIVVINGLLLLSLASILVKIG"
     gene            2438..>4718
                     /locus_tag="PLI76_09650"
     CDS             2438..>4718
                     /locus_tag="PLI76_09650"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOI62337.1"
                     /translation="MDALRAYAGVPGLLRKVIDENDGDAWAEITGKIDYIYTHIGYAL
                     RALDRETGFIGEVQSQVRSGKKLLFKPNLVGPQVIDPVTHGEDLGAPICTDWSVMAAL
                     MRWFHDNLDIDYHQMALGEASTSSLLLESVFGRQAGRSITSEAIFEGRSGDFYGGWGF
                     YFVRRYLAERHSPSHTDNPMRGYEESVAGRYFPPGRAGDRLMVYDLNKLCDDLSRGRT
                     VPVPGGANFQEITLHKAIVGGDPRDADDRGDYPGCVLVNVPKMKIHAQDLITNAVKNL
                     GIGLYPTQCPAYTGETSWKYALPSSATPSYKAKLPHMPWVAEVDTASNLPVKDENGDY
                     VVTKTMGMPGTQADVIRAVQNQQVFMVHVSDAIDMINLNHNPEGIAVRIPEGYIWSSL
                     DCVALDHLCARYCFKTVPMAEGLKLKEENGWATEFVHHVPVAKVEGRNIVTAEGLDSP
                     LFRYNLYRYAEERGVGRQQYYVTGWDGITGTPLASLLGHPGRIEDAAFVELMTKTMYY
                     NPTCMLWDMQKTLLCYAEAHDRLTGSSILEQFMEGFDENRDGIIDYDENGQKGFWTLG
                     FSILSHALDLEMTGDYGMLEGRFYQVANLSLKHTDRDWNPQGHDFAREYMLVWIATQA
                     YDMSKAETVSDDPFVPGMQWGGGMWPSWDLAAWHLLSGLVYGGTSPDQVGPGSLYGTA
                     FRYADKTLHNGAYTGSVDQGVSDPRAVATYFRAVSNGADPLDFILYVPAGFGSLAGTK
                     IPNVEETNDPGRIFTAHFAG"
BASE COUNT          935 a         1386 c         1404 g          993 t
ORIGIN      
        1 cttcagggta tccaggtccc gcccggttat cgggcctgcc gcctcgaagt tcatggtgag
       61 gaaggtgctc ccctcgtagc tgacggtcgt tgcatagtga ggccgtacga tatgcagatc
      121 ttcgatatcc gcgtatatct tcgggatgcc ggtctgctcc cgccccccga ggatgggggc
      181 ggtcttgttc tcccagacaa ccagcgtgta ggcaccgtcg agctcgtcct tcttcccgtg
      241 gaaccggacc ggtgccgaga cgttgatcag gttgtactgg ccgccgtgca tccagttgat
      301 ctcggtgaac ttgttgaacg caacctgcac ctcgggggcc aggagttcga atccctcggg
      361 aatatagttc tcaagaagat ctctctcggt ctcgtaactg acggcgagac cggttgcctt
      421 ctgcgtgatc agggttcccg ggtcgaattt gccgcctccg aagtggaccg gcatcaagta
      481 cgtaaaatca tcctgcggtc ggaacataac atcctccaat ctgtctcatg tcatcccata
      541 agagatatgt gttttggatc ttgctttccc cggggagcga tttgaaaaga tacattttca
      601 tgtcacaata tgcgtaacaa acggcaattc ttcgtattca tagcaacgcc accaggccgg
      661 cagagcaatg cggcaaagag aatgccgacc agggccggga aacacctgca acgcagccgg
      721 agcggatgca tacttataaa taaactccgg atgcagatca aaatatttac gtaatcgtga
      781 tatatccggc gatggacgga tagagcgagg tggatacaat tatgggattt accagtcagt
      841 caggggaaaa aaacaacccg gttgtgagag cagagtccga actctctctc gaagtcgtca
      901 tcctgatcgt ctttggagtg ttcatgctcc tcttcggtct gcttctcttc aggattcaga
      961 cgggcgaact tccgtatgcc ccggacagca cctacgggct gtttctcgtg atcgtctcgt
     1021 ttcaggtcat cacgatgggg aagaccccgt tcggcgacct ccggcgttca tgggcggtca
     1081 tagtgctcgg ggtatgcacg gcgatcgtcg ggatggtcgc ctgcttcatt cccgggctgc
     1141 tggccgagcc ggtccgcatc cttgtcggca tactcctctc tgccgggggg gtcgcgctcc
     1201 ttgtgcagtt gttcttctct gaagataagg cccgggtatg gctgaaggtc cccggagccc
     1261 tccagcacct gacggtcgca tgcggccttg tgtacgtggt ctcgatcgtt ctcggcatca
     1321 tcacgctcct tcccggcatc acgaccgaca ccgtgactgc agtcttcctc gtcatctacg
     1381 gcgtcgggtt cttctacctc tcctggagca tccagaaggc cggccggctc taccctccgg
     1441 gggaagcaga atgaccccga acgcctccac tgcaaccggc ggcaccggcc tgttcgggga
     1501 ggcctccctt cccctctcga ttgccctgat catcccggtc ggcgttcttc tggtcctcct
     1561 cggtctgctt ctcttcccgg tgaaccaggg gttgatcccg ttctccccgg atggtcagct
     1621 cggcctgctg ctcgtcatca tggccatcca gatgctggcc ctgggagaga ccccggtagg
     1681 ccagtacagg cgctcgtggc tcctgatcgg catcgggatc gtctttgccg ctctggggat
     1741 cttctcctgc atcgttccgg gcatcctgac cgacgcaata cgggtgctcc tggcgttctt
     1801 gaacatagcc gggggagcga tactcctcgg gaagcggttc ctcccgatgc tgcagggcac
     1861 cgggagcgcc gccgaactgg ctcaccttcc gcccatctac aaacggctcc tcggtaccct
     1921 gacggtcttg aacgtcgtgg cgatcgcgtt cggcatcagc atgcttctgc cgggcctcgt
     1981 tccgggcatg gtcatcgcag ggatcgtcgt gatcaacggc ttgctcctcc tctccctggc
     2041 atccattctc gtaaagatcg ggtaacccgg gatcccgcca tccttttttc tgcttcccaa
     2101 tcgccgtttc ccaccggaat gcagtccggg tgccggcttt atgctcccgg tgggcatctt
     2161 ggtgaccgga aaggaatcag aaggcatata atggtacgca gtgtacgctg aatcatccgg
     2221 aatcatcaaa tagggtccga catcgaaggt gaaggcctcg cgcggagcta cgggagattc
     2281 tgatgttgtt cagagcgatt tgattgcccc cattcagact gagaggattg agaggatcat
     2341 gaccacactc gaacgttccc tgaaaggaac cccggtaaga tcggatatcg ggagaacgaa
     2401 gaccgattcc gatggatctc ctgtctgcgg cacaaggatg gatgccttac gggcctatgc
     2461 cggtgttccc ggactgctcc ggaaggtcat cgatgagaac gacggtgacg cctgggcaga
     2521 aattacgggg aagatcgact acatctacac gcatatcggt tatgccctgc gcgctctcga
     2581 ccgggagacc ggttttatcg gcgaggtgca gtctcaggtg cgatccggca agaaactgct
     2641 cttcaagccc aacctggtcg gaccgcaggt tatagatccc gtgacacacg gcgaagacct
     2701 tggcgctcca atctgcaccg actggtcggt gatggccgcc ctgatgcggt ggtttcacga
     2761 caacctcgat atcgactacc accagatggc gcttggggaa gcctccacgt cttcgctcct
     2821 cctggagagc gtttttggcc ggcaggccgg gcggagcatc acctcggagg cgatcttcga
     2881 ggggcgaagc ggggatttct acggcgggtg gggattctac ttcgttcgcc gctacctcgc
     2941 agagcgccac tctccttccc acaccgacaa ccccatgagg gggtatgaag agagcgtggc
     3001 cggcaggtac ttcccgccgg gcagggcggg ggaccggttg atggtctacg acctgaacaa
     3061 gctgtgcgac gatctgtcac ggggaaggac ggttccggtt cccggcgggg cgaacttcca
     3121 ggagatcacc ctgcacaaag cgatcgtggg cggcgacccc cgtgacgccg acgatcgcgg
     3181 ggactatccc ggctgcgtcc tggtcaacgt cccgaagatg aagatccacg cgcaggatct
     3241 catcaccaac gcggtgaaga acctgggcat cggcctctac ccgacgcaat gccccgcata
     3301 cacgggagag acttcgtgga aatacgcgct gccctcctct gcaacgccga gctacaaggc
     3361 gaaactgccg catatgccct gggtcgccga agtggatacc gcctccaacc tgccggtgaa
     3421 ggatgagaac ggagattacg tcgtgacgaa gaccatgggg atgccgggga cccaggcaga
     3481 cgtcatcagg gccgtgcaga accagcaggt cttcatggtg cacgtctccg atgcgatcga
     3541 catgatcaac ctcaaccaca accccgaagg catcgccgtc aggattcccg aaggatacat
     3601 ctggtcgtct ctggattgcg ttgcactgga tcatctctgc gcacggtact gtttcaagac
     3661 cgtccccatg gccgaagggc tcaaactcaa ggaggagaac ggctgggcca cggagttcgt
     3721 ccatcatgtc ccggtagcaa aggtcgaagg gcgaaatatc gtgaccgcag aagggctcga
     3781 ctcgcctctc ttcaggtata acctctaccg gtacgccgaa gaacgggggg tgggccggca
     3841 acagtactac gtcactgggt gggacggcat caccgggacg ccgctcgcat ctctcctcgg
     3901 gcatcctggg aggattgagg atgcggcctt cgtcgaactc atgacgaaga cgatgtacta
     3961 caacccgaca tgcatgctgt gggacatgca gaagacgctc ctctgctacg cagaggcgca
     4021 cgatcgcctg accggctcct cgatccttga acagttcatg gaggggttcg acgagaaccg
     4081 cgatggcatc atcgattacg acgagaacgg acagaaaggg ttctggacac tggggttcag
     4141 catcctgtcg catgcgctgg atctcgagat gaccggagac tatggtatgc tcgaagggag
     4201 attctaccag gtagcaaacc tctccctgaa gcataccgat agagactgga atccgcaagg
     4261 ccacgatttc gcccgtgagt acatgctggt gtggatcgcg acgcaggcct acgatatgtc
     4321 gaaagccgag acggtgagtg acgacccctt tgtgccgggg atgcagtggg gagggggcat
     4381 gtggccgagc tgggatctcg cggcatggca cctgctgtcc gggctcgtgt acggtggaac
     4441 gtctccggac caggtcggtc ctggttccct ctacgggacg gcgttcaggt atgccgataa
     4501 gaccctgcat aacggtgcat ataccggaag cgtcgatcag ggggtctctg atccacgcgc
     4561 cgtcgccaca tatttcaggg ctgtttcaaa cggagcggat cccctggact tcattctcta
     4621 tgtgccggcg gggttcggga gtctcgcggg tacgaagatc cccaacgtgg aggagacgaa
     4681 cgatccgggc aggatattca cggcccattt tgccgggg
//
LOCUS       DAOSEE010000150         4714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_6410_length_4714_cov_202.507405, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000150 DAOSEE010000000
VERSION     DAOSEE010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6410_length_4714_cov_202.507405"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..673
                     /locus_tag="PLI76_09655"
     CDS             47..673
                     /locus_tag="PLI76_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HOI62338.1"
                     /translation="MIPLMLDLTGRRVLIFGGGDVGARKAAYFAHEAEVTVISRSFSP
                     DLDGLAIRRQETDLAALSDEALQSLLAGTFLAVAATPDAALNDRIGRVCAGSGVLFNN
                     AAGKPGDIVIPSVVRGSRYLVAISTRGKSPAVSRYIRMRFEAEYADLDRMIDLQDDMR
                     SMLKEVEPVQAERSRILWSILRDEEVWRALASDYDGARTMAIERYLHA"
     gene            666..1940
                     /gene="hemA"
                     /locus_tag="PLI76_09660"
     CDS             666..1940
                     /gene="hemA"
                     /locus_tag="PLI76_09660"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HOI62339.1"
                     /translation="MPDPAATTPLALAGVNHHTADIATIEAFRFPDEAAFLHEARERF
                     RGALLLQTCNRVEVLVQGDARSLEGFLAEQGRRGFAVIEGEAVPRHLLELAAGIDSLV
                     VGEDQILGQLKRALAVTEEAGAASSVIKICINKAVHVGVRVRRQTEINRGAVSVGSAA
                     VTLAEKLLGTLRDRHILVIGSGEMGLLVTQALCAKDLTAIYVANRTYERAVMLAEKIG
                     GRAVNFRDLYRYIALSDVVISCTAAPHPVIRTEDIREVMEKRLWPLDPAPRHLILIDI
                     AQPRDIEDEVRSVEGVHLFTIDDLRSINDAAMDSRRSEADRARGIIDEELDRFVRLLR
                     RTAADETLALLYTWAESIRTRERDRALARLGEEDDRTVEVIDDLTRALTKKLLSDATA
                     AIRASAECGDTARADGLMRAITRGEPCFQNDE"
     gene            1919..2902
                     /gene="hemB"
                     /locus_tag="PLI76_09665"
     CDS             1919..2902
                     /gene="hemB"
                     /locus_tag="PLI76_09665"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HOI62340.1"
                     /translation="MFPERRMRRIRRRNIRPLLRETELQKTDLVMPVFVDESIRTPLA
                     IASMPGQFRHPVDGVADYCERLLGAGIRAVILFGIPAAKDSEATGAYAADGVVQRAAR
                     SIKERLPQMVVATDVCACEYTDHGHCGIVGETIDGPDLKNDPSLALMARIAVSHAESG
                     ADIVAPSCMLDGMVQAIREALDAAGYQDVLIMSYSSKFASALYGPFRDAADSGFSFGD
                     RTTYQIDPGNAREAVMESELDAAEGADILMVKPAGIYLDILASVADLGLPVAAYQVSG
                     EYAMIKAAGERGWLDERAVALESLTAIKRAGADLIITYFAEDAARWLDEEQ"
     gene            2889..4136
                     /gene="hemL"
                     /locus_tag="PLI76_09670"
     CDS             2889..4136
                     /gene="hemL"
                     /locus_tag="PLI76_09670"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HOI62341.1"
                     /translation="MKSSDLFARAKTLMPGGVSSPVRAIKPYPFYVERAAGSRIATVD
                     GADLIDCCLGYGPLILGHAHPVIREAIKRQLEKGWLYGTPSPLELDLAEVITGDHPGI
                     DMVRFVSSGSEATMAAIRLARGYTGKQDIVKVEGGFHGAHDAVLVKAGSGATTLGVPD
                     SAGVLADLTAHTRQVPYNDPEALEAILAGNDDVAAFILEPIMGNIGPILPDSGYLADV
                     REITAAHGVLLILDEVITGYRVGIGGAEVLYGVKPDIATFGKIIGGGLPIGAFGGRRD
                     IMDLIAPSGPVYQAGTFSGNPASLAAGIATLRHLHDHPDSYRRLDDATRAIEEVAVDA
                     GRGSFVRMGSLFKHFFRDTPPRDYREVKECDTEAFGRFWKAMLEAGIFLPPSQFETNF
                     LSAAHSDQDIEQIAGAYGSCLFA"
     gene            4121..>4714
                     /gene="hemC"
                     /locus_tag="PLI76_09675"
     CDS             4121..>4714
                     /gene="hemC"
                     /locus_tag="PLI76_09675"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HOI62342.1"
                     /translation="MSLRIGTRGSALALAQTNKVAGMLADRGIETEVVTITTEGDTAT
                     GVPLHAIGGQGVFVRALDDAILRGDIDAAVHSMKDIPAARPAGLACSAVLERDSPADF
                     LAHDLPLDAVRVVGSSSTRRRAQLLRYDPALEVKQLRGNVDTRIRKLREGQYDAIVLA
                     EAGLERLGLSLPGEALPLDQFVPSPNQGTVAVVCRDDP"
BASE COUNT          815 a         1574 c         1568 g          757 t
ORIGIN      
        1 ggtttttcaa caccttaatc tattccgttc accacactat agaagaatga tccctcttat
       61 gcttgacctg acgggcagga gggtgctcat attcggggga ggcgacgtcg gtgcccggaa
      121 ggctgcatac tttgcgcatg aggcggaggt gacggtgatc agccgttcat tctcgccgga
      181 ccttgacggt cttgcgatcc ggcggcagga gaccgatctt gccgcccttt cggacgaggc
      241 gctccagagc ctcctcgcgg gcaccttcct cgcggtagcc gcgacgccgg atgcggcact
      301 caacgaccgt atcgggagag tatgcgccgg atccggcgtc ctcttcaaca acgccgccgg
      361 taaacccggc gatatcgtca tcccttcggt cgtccggggc agccgctacc tggtcgcgat
      421 cagcacccgc gggaagagcc ctgccgtatc gcgctacatc cgcatgcggt ttgaggccga
      481 gtatgcggac ctcgaccgga tgatcgacct gcaggatgat atgcgctcga tgctcaaaga
      541 ggtcgaaccg gtgcaggccg agcggtctcg tatcctctgg agcatcctcc gcgacgaaga
      601 ggtctggaga gcgcttgcct ccgactacga cggggcccgc acgatggcga tcgagaggta
      661 cctgcatgcc tgatccggcc gcaacgaccc cgcttgctct tgcgggcgtg aaccaccaca
      721 ccgctgacat cgccacgatc gaggcgttcc ggttccccga cgaggcggcc ttcctccacg
      781 aggcccggga gcggttcagg ggcgccctcc tgctccagac ctgcaaccgc gtcgaggtcc
      841 tggtgcaggg cgacgcgcgg agccttgagg ggtttcttgc ggaacagggc cggcgcggtt
      901 ttgcggtcat cgagggggag gccgtgcccc gccatctcct ggaactcgct gccggcatcg
      961 actctcttgt cgtcggcgag gaccagatcc tcggccagct caaacgggcg ctcgcggtca
     1021 cggaggaggc gggggccgca agcagcgtca tcaagatctg catcaacaag gcggtgcacg
     1081 tgggagtcag ggtccggcgc cagacagaga tcaaccgggg tgcggtctct gtcggttccg
     1141 cagccgtgac gcttgccgag aaactcctcg gcacccttcg cgaccggcac atcctggtca
     1201 tcgggagcgg ggagatgggg ctgctggtga cccaggcgct ctgtgcgaag gacctgacgg
     1261 cgatatacgt cgcgaacagg acatacgaac gcgcggtgat gcttgcagag aagatcgggg
     1321 gccgggcggt caacttcagg gacctctacc gctacatagc gctctccgac gtcgtcatat
     1381 cctgcaccgc cgcgccgcac ccggtcatcc gcacggagga tatacgggag gtgatggaga
     1441 agcgcctctg gccgctcgat ccggcccccc gccacctgat cctcatcgac atcgcccagc
     1501 cccgcgatat cgaggatgag gtgcggtccg tcgagggcgt gcacctcttc accatcgacg
     1561 acctgcgcag catcaacgat gccgccatgg attcccggag gagcgaggcc gaccgggcac
     1621 gggggatcat cgacgaggag ctcgaccgct tcgtccgtct cctccggcgg acggcggccg
     1681 acgagacgct tgccctcctc tacacctggg cggagtcgat acggacacgc gagcgcgacc
     1741 gggcgctcgc ccgcctggga gaggaggacg accgcacggt ggaggtcatc gacgacctga
     1801 cgcgggcgct cacgaagaag ctcctctcgg acgcgaccgc agccatccgt gcgagtgcgg
     1861 aatgcggcga tacggcgaga gccgatggcc tgatgagggc gatcacccgg ggagaaccat
     1921 gtttccagaa cgacgaatga ggcggatacg gcggcggaac atccggccgc tcctccgcga
     1981 gacagaactt caaaagaccg acctcgtcat gccggtcttt gtagacgaat cgatccgcac
     2041 accactggcc atcgcctcga tgccggggca gttccggcac cccgtggatg gggtcgccga
     2101 ctactgcgag cgcctcctgg gcgccggcat ccgcgcggtg atcctctttg gcataccggc
     2161 agcaaaggac agcgaggcga ccggggccta cgcggccgac ggcgtcgtcc agcgggccgc
     2221 gagatcgatc aaggagcgcc tgccgcagat ggtggtcgct accgacgtct gcgcctgcga
     2281 gtacaccgac cacggccact gcggcatcgt cggagagact atcgacggcc ccgacctcaa
     2341 gaacgatccg tcgcttgccc tgatggccag gatagccgtc tcccatgccg agagcggcgc
     2401 cgatatcgtc gcgccgtcgt gcatgctcga cggcatggtg caggcgatca gggaggcgct
     2461 cgacgctgcc gggtaccagg acgtcctgat catgtcctac tcctcgaagt tcgcgagcgc
     2521 cctctacggg ccgttccgcg acgccgcgga ctcggggttc tcgttcggcg accggacgac
     2581 ctaccagatc gacccgggca acgcccgcga ggcagtgatg gagtcggaac tcgacgccgc
     2641 cgagggggcc gatatactga tggtgaagcc ggccgggata tacctcgata tccttgcatc
     2701 ggtggcggac ctcggcctgc cggtggcggc ttaccaggtc agcggggagt acgccatgat
     2761 caaggccgcc ggagaacgcg gctggctgga cgagcgggca gtcgccctcg agagcctcac
     2821 cgccatcaag cgtgccgggg cagacctgat catcacttac tttgccgaag atgcagcgag
     2881 gtggctcgat gaagagcagt gatctcttcg cacgggcaaa gaccctgatg ccgggcggcg
     2941 tcagcagccc tgtccgggcc atcaagccct acccgttcta cgtggagcgg gcggcgggct
     3001 cgcgtatcgc gaccgtcgac ggggcggatc tcatcgactg ctgtctcggc tacggtccgc
     3061 tcatcctcgg ccacgcgcac ccggtcatcc gggaggcgat aaagcgccag ctcgagaagg
     3121 gctggctcta cggcacgccg tcgccccttg agctcgacct tgcggaggtc atcaccggcg
     3181 accaccccgg catcgatatg gtccggttcg tctcgtcggg ctcggaggcg acgatggccg
     3241 cgatccggct cgcccgcggc tacacgggca aacaggacat cgtcaaggtc gagggcgggt
     3301 tccacggcgc ccacgatgcg gtcctcgtga aggccgggtc gggagcgacc accctcggtg
     3361 tgcccgactc cgccggcgtc ctcgccgacc ttacggcaca cacccggcag gtcccgtaca
     3421 acgatcctga ggcgctggag gcgatccttg ccgggaacga cgacgtcgcc gcgttcatac
     3481 tcgagccgat catggggaac atcgggccga tcctccccga cagcggctac ctcgccgacg
     3541 tccgcgagat caccgccgcc cacggggtcc tcctcatact cgatgaggtg atcaccggct
     3601 accgggtcgg tatcggcggc gccgaagtgc tctacggggt aaagcccgat atcgccacgt
     3661 tcggcaagat catcggcggc ggccttccca tcggggcatt cggggggcgg cgcgacatca
     3721 tggacctgat cgcgccttcg ggacccgtct accaggcggg gacgttcagc ggcaacccgg
     3781 caagccttgc ggcggggatt gcgaccctcc gtcacctcca cgaccatccg gatagctacc
     3841 ggcggctcga cgacgccacg cgggcgatcg aggaggtcgc cgtcgatgca gggcgcggtt
     3901 cgttcgtcag gatgggatcg ctcttcaagc acttcttccg cgatacaccg ccgcgagact
     3961 accgggaggt caaggaatgc gacacggagg cattcgggcg gttctggaag gcgatgctcg
     4021 aggcgggcat cttcctcccg ccctcgcagt tcgagacgaa cttcctctcc gcggcgcata
     4081 gcgatcagga tatcgaacag atagcgggcg catacggatc atgtctcttc gcataggcac
     4141 acgggggagc gctcttgcgc ttgcccagac gaacaaggtc gccgggatgc tcgccgaccg
     4201 gggcatcgag acggaggtcg tcacgataac gaccgagggc gacaccgcga cgggagtccc
     4261 gctccacgcc atcgggggac agggggtctt tgtccgggcg ctcgacgacg ccatcctccg
     4321 cggcgatatc gacgctgcgg tgcacagcat gaaggatata ccggcggcgc ggccggcagg
     4381 gcttgcctgc tctgcggtgc tcgagcgtga ctcgccggcg gatttcctcg cgcacgacct
     4441 ccctctcgac gcggttcgcg tcgtcggatc gtcgagcacc cggcgccgcg cccagctcct
     4501 ccgctacgac ccggcgcttg aggtgaaaca gctccggggg aacgtcgata cacggatacg
     4561 gaagctccgc gaggggcagt acgacgccat cgttctcgcg gaggccggcc tcgagcggct
     4621 cggcctcagc cttcccgggg aggctcttcc cctggatcag ttcgtcccgt ccccaaacca
     4681 ggggaccgtc gcggtcgtct gccgggacga cccg
//
LOCUS       DAOSEE010000151         4711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_6413_length_4711_cov_237.105241, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000151 DAOSEE010000000
VERSION     DAOSEE010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6413_length_4711_cov_237.105241"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="PLI76_09680"
     CDS             complement(<1..549)
                     /locus_tag="PLI76_09680"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844427.1"
                     /note="catalyzes the ATP-dependent breakage, passage, and
                     rejoining of double-stranded DNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI"
                     /protein_id="HOI62343.1"
                     /translation="MSREEMDALARERLVGIARGWYDQMRDGIVPYIRLPTRTKQNIE
                     YDDESEVWKYGDKESTRSAATEKSAVHLLKMAYVIGFLKQQLAENRSSTLRELYYISE
                     GWKRAKFAAQDESNLLIEDLEIITEVQREAFHLRPEEDGASIFGPLRIREATRRGVKE
                     MHCQEDVGEAGYPIPNNVDNLDF"
     gene            complement(542..2344)
                     /locus_tag="PLI76_09685"
     CDS             complement(542..2344)
                     /locus_tag="PLI76_09685"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HOI62344.1"
                     /translation="MVLAEDLAKQQRSISVAEFFEKNKHLLGFDSPTRGIITTIKEAV
                     DNALDACEEAEVLPDIFVGVRRVDADVYRITVEDNGPGIVPENVPPVFGKLLYGSRFH
                     QIRQSRGQQGIGISAAVLYAQLTTGTPALVVSRTGAKARAHRFELMIRTETNEPEIVS
                     HEEIDWDRMHGTRLEIQFKSTLAAKKRLVDYLRLTAIVNPHARITADIDGEVTTFERV
                     ASEVPPCPKPVLPHPHGIEFGALKRIAAASTGTIDEFLVGSFSRVGKKTADDMIALAG
                     LKPSRKVKNLDADELRRLLDAMQSVKVPAPPAQQCLSPIGEDLICRGLEKEIALDFVR
                     ARTRPSAVHGGHSFIIEAAIGYGGKIPAEGNAQIFRFANRVPLLYQQGACAITNCISQ
                     VNWKNYGLSQQGLPVGPALVMVHVASTNVPFTSESKDAVASIPEIEREIVLVLQELGR
                     ELKAYLSRRERKKQQDDRARAICSIIPEIAAKVSEIVELPLVDTSPIEGRIMRRVVAK
                     KRTHDGKVRIDVNNYTGGEVTLVLYNLSRDAATDANPRPDFVDEIDGEYNKVWRCTLD
                     AGAAWQVVYTGKGDGSLDLRGVEDGRKVVVDLDV"
     gene            complement(2331..3563)
                     /gene="hisS"
                     /locus_tag="PLI76_09690"
     CDS             complement(2331..3563)
                     /gene="hisS"
                     /locus_tag="PLI76_09690"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOI62345.1"
                     /translation="MLQKPRGTRDFLPDEMERRRLIERRMREAAGRWGYREVSTPDFE
                     HLELFTMKSGEGIIQEMYVFEDKGGRQMTLRPEVTAAVLRMYVNEGKVLPKPIRWCYF
                     ADCFRYERPQKGRYRQFWQFGVELIGADTPSADAEVVMLADDVLRSTGVTFDLHVGHL
                     APMKHLLADLAPGDQRAIMACLDKHDTAGLEAALVEKNLTHLAEPLAALSKCRNVSEV
                     FEIAGDIPERARIEEIFSLLDSQDIDYRPDFGIARGLDYYTGMVFEGFAKNLGAENQI
                     IGGGAYRLAHLFGGDDVASSGFAIGFDRVVVSIGDFPLVDEPIVGVVCTPEARARALD
                     VARAFRSAGVRAEIDLMQRGMGAQVSHAAKTADFAVILGKREVEAGTVTLRDLHSGKQ
                     QERSLEDAIAEVARHGSC"
     gene            complement(3580..3912)
                     /locus_tag="PLI76_09695"
     CDS             complement(3580..3912)
                     /locus_tag="PLI76_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI62346.1"
                     /translation="MSYLVYVAGFGVAVTVFLWLRDLRIFYRTGLSGYRTAAYWGVPY
                     TALALLGFFVTAYAEAWEFLGLGLVLLALYLQGRVDRENVWQNEGTAERFFGRAKRTK
                     DKGSRKRL"
     gene            3960..>4711
                     /gene="larB"
                     /locus_tag="PLI76_09700"
     CDS             3960..>4711
                     /gene="larB"
                     /locus_tag="PLI76_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HOI62347.1"
                     /translation="MLPNATIREILRMYRSGEVSEDEAAAAIEGLRIDVLDGMARIDA
                     GRSVRCGMPEVVLAEGKDPLTFAEIMLAQVKTAGRCIATRVSPAHIEALRSRLPSDIR
                     LEHRKAARAVILSTGNGPDPRGGTVAILTAGTSDIPVAEEARLIAEEMGCDVRTAYDV
                     GVAGIHRLFSALKDLIPADVFIVAAGREGTLPAIVAGLVDRPVIGVPVSTGYGYMGGG
                     EAALASMLQACSVLAVVNIDAGFTAGAFAAQVA"
BASE COUNT          767 a         1550 c         1489 g          905 t
ORIGIN      
        1 gaaatcgagg ttgtcgacgt tgttcgggat ggggtagccc gcctccccga catcctcctg
       61 gcagtgcatc tccttcaccc cgcgccgggt cgcctctctg atccgcaagg gcccgaagat
      121 cgaggcgccg tcctcctccg gccggaggtg gaacgcctcc cgctgcactt cggtgatgat
      181 ctcgaggtcc tcgatcagga ggttgctctc gtcctgggcg gcaaacttcg ctctcttcca
      241 cccttcggat atgtagtaga gctctctcaa ggtcgacgac cggttttcgg caagctgctg
      301 ctttagaaac ccgatgacgt aggccatctt caggaggtgg accgcgctct tctcggtcgc
      361 cgccgaccgg gtgctctcct tatcgccgta cttccagacc tcgctctcat cgtcgtactc
      421 gatattctgt ttcgtccgcg tcgggagccg gatatagggc acgatgccgt cccgcatctg
      481 gtcgtaccac ccacgggcga tacccaccag ccgctcgcgc gcaagtgcat ccatctcttc
      541 tctagacatc gagatccacc accactttcc tgccgtcctc gacaccacgc agatcgagcg
      601 acccatcgcc cttccccgtg tagacgacct gccaggccgc gccggcatcg agcgtgcacc
      661 gccagacctt gttgtactcc ccgtcgatct cgtccacgaa gtccggccgg gggttcgcat
      721 cggtggccgc atcgcgggag aggttgtaga ggacgagcgt gacctcgcca cccgtgtagt
      781 tgttcacgtc gatacggacc ttcccgtcgt gcgtccgctt ctttgcgacg acacggcgca
      841 tgatcctccc ctcgatcggg gatgtatcca ccaggggaag ttcgacgatc tcgctcactt
      901 tcgcggctat ctcggggatg atcgagcaga tcgcccgggc ccgatcgtcc tgctgcttct
      961 ttctctcccg gcgggagagg taggctttca gttcccggcc gagctcctgg aggacgagca
     1021 cgatctcgcg ctcgatctcg gggatggagg cgaccgcatc cttgctctcg ctcgtgaagg
     1081 ggacgttcgt ggatgccacg tggaccatga cgagcgccgg gcccacgggg agtccctgct
     1141 gcgagagacc gtagttcttc cagttcacct gggatatgca gttcgtgata gcgcaggcgc
     1201 cctgctggta gaggagcggg acgcggtttg cgaaccggaa tatctgggcg ttcccctctg
     1261 cggggatctt gcccccgtag ccgatcgcag cctcgatgat gaacgagtgc ccgccgtgga
     1321 ccgcgctcgg gcgggtccgg gccctgacga agtcaagggc gatctccttt tctagccccc
     1381 ggcagatcag atcctccccg atgggggaga ggcactgctg cgccggcggt gccggaacct
     1441 tcacgctctg catggcgtca agaagcctcc tcagttcgtc ggcatcgagg ttcttcacct
     1501 tccgggaggg tttgagcccg gcaagagcga tcatatcgtc cgcggtcttc ttcccgaccc
     1561 gggagaaact cccgacgagg aactcgtcga tcgtcccggt gctcgcggcc gcgatccgct
     1621 tcaatgcccc gaactcgatc ccgtgcgggt gggggaggac gggtttcggg cacggcggga
     1681 cttcgctggc cacccgctca aacgtcgtca cctcgccgtc gatatcggcc gtgatacgcg
     1741 cgtgcgggtt gacgatcgcc gtcaggcgga gataatccac cagccgcttc ttcgcggcaa
     1801 gggtgctctt gaactggatc tcaagcctcg tgccgtgcat ccggtcccag tcgatctcct
     1861 cgtggctcac gatctcgggc tcgttcgtct cggtccggat catcagctcg aaccggtgtg
     1921 ctctcgcctt cgctcccgtc cgggagacga cgagcgccgg ggtgccggtg gtgagctgcg
     1981 catacagcac cgccgccgat ataccgatac cctgctgccc ccggctctgc cggatctggt
     2041 gaaaccgcga gccgtagagg agcttcccaa agaccggggg aacgttctcc ggcacgatcc
     2101 cggggccgtt gtcctcgacg gttatccggt agacgtcggc gtcgaccctc cgtacgccga
     2161 cgaatatgtc ggggaggacc tcggcctcct cgcaggcgtc gagcgcgttg tccaccgcct
     2221 ccttgatggt ggtgatgatc ccgcgcgtcg gggagtcgaa gccgagaagg tgcttgttct
     2281 tctcgaagaa ctccgcgacg ctgatgctcc gctgctgctt cgccaggtcc tcagcaagaa
     2341 ccatgccgtg ctacctcagc gatggcgtcc tcaaggctcc tctcctgctg cttgcctgag
     2401 tggagatccc tgagggtcac cgtccccgcc tcgacctcgc gcttcccgag gatcacggcg
     2461 aagtcggccg tttttgcggc gtgagagacc tgggcgccca ttccccgctg catgaggtcg
     2521 atctcggccc tcacccctgc gctacggaag gcgcgggcta catcgagcgc ccgtgcccgt
     2581 gcctcgggcg tacagacgac gccgacgata ggttcatcta cgagcgggaa atccccgatc
     2641 gagaccacga cccggtcgaa ccctatagca aaccccgagg aggccacgtc gtcgccgccg
     2701 aagaggtggg cgagccggta ggcgccgccg ccgatgatct ggttctccgc accgaggttt
     2761 ttcgcgaacc cctcaaagac catccccgtg tagtagtcaa gtccccgggc gatgccgaag
     2821 tccggccggt agtcgatatc ctgggagtcg aggagcgaaa atatctcctc gatccgcgcg
     2881 cgttcgggga tgtcgccggc gatctcaaag acctccgaaa cgttgcggca cttcgagagc
     2941 gccgcaaggg gttcggcaag atgggtgagg tttttctcga cgagcgccgc ctcgagccct
     3001 gcagtatcgt gcttgtcgag gcaggccatg atcgcccgct ggtcgcccgg cgcgaggtcg
     3061 gcgaggagat gcttcatcgg cgcgaggtgg ccgacgtgga ggtcgaacgt gacgccggtc
     3121 gatcggagca catcatcggc gagcatgacc acctcggcgt cggcggacgg agtatctgcg
     3181 ccgatgagct cgacgccgaa ctgccagaac tgccggtacc ggcccttttg tgggcgttca
     3241 tagcggaaac agtcggcgaa gtagcaccag cggatgggct tcgggaggac cttgccctcg
     3301 ttgacgtaca tccggaggac cgctgcggtc acctccggcc ggagcgtcat ctgcctcccg
     3361 cccttgtcct cgaagacgta catctcctgg atgatccctt cgccggactt catcgtgaag
     3421 agttcgaggt gctcgaagtc gggcgtgctg acctcccggt atccccaccg gccggctgct
     3481 tcccgcatcc gccgctcgat cagccgtcgc cgttccatct cgtcgggtaa aaagtcccgt
     3541 gttccccgtg gtttctgaag cattcggtat tctcctgaac taaagtctct tgcgcgaacc
     3601 cttatctttt gtgcgctttg ccctgccgaa gaaccgctcg gcggtgcctt cgttctgcca
     3661 tacgttctcc cggtctaccc ggccctggag gtagagggct aacaggacca gcccaagccc
     3721 caggaactcc cacgcttcgg cgtacgcagt cacgaagaaa ccgagcaggg caagcgccgt
     3781 ataggggacc ccccaatatg ccgccgtccg gtagccggat agaccggtcc ggtagaatat
     3841 ccgcaggtcg cggagccaca ggaagacggt gacggcaacg ccgaaccctg cgacgtagac
     3901 gaggtaggac atgagatgaa gtaatattat acttccccac ctatttttgt tatcgaacca
     3961 tgctgccgaa tgccaccatc agggagatcc tccggatgta tcgtagcggt gaggtatctg
     4021 aggacgaggc ggcggccgcc atcgaggggc tccgcatcga cgtgctcgac gggatggccc
     4081 ggatcgatgc cgggcggagc gtgcgctgcg ggatgcccga ggtggtgctc gccgagggaa
     4141 aggatcccct cacgtttgcg gagatcatgc tcgcgcaggt gaagacggcc ggccggtgca
     4201 tcgcgacgag ggtctcgccg gcgcatatcg aggccctccg gtcccgcctg ccgtcggata
     4261 tccggctgga acatcggaag gcggcgcggg cggtcatcct ctcgaccggc aacggacccg
     4321 acccccgggg cggaaccgtc gccatcctca ccgcggggac atcggatatc cccgtcgccg
     4381 aggaggcgag gctgatcgcc gaggagatgg ggtgcgacgt caggactgca tacgatgtcg
     4441 gcgtggcggg gatccaccgc ctcttctcgg ccctcaagga tcttataccg gcggatgtct
     4501 tcatcgtggc cgccggccgg gagggaacgc ttcccgcgat cgtcgccggc ctcgtcgacc
     4561 gtccggtgat cggcgttccc gtgagcaccg gctacggcta catgggcggc ggcgaggcgg
     4621 cgctggcgag catgctccag gcatgctcgg tgcttgcggt cgtgaacatc gacgccgggt
     4681 tcacggcggg cgcgtttgcc gcgcaggttg c
//
LOCUS       DAOSEE010000152         4701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_6425_length_4701_cov_214.196083, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000152 DAOSEE010000000
VERSION     DAOSEE010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6425_length_4701_cov_214.196083"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(465..1895)
                     /locus_tag="PLI76_09705"
     CDS             complement(465..1895)
                     /locus_tag="PLI76_09705"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOI62348.1"
                     /translation="MKLLINGEWRDAASGKAFAVRNPATGDMLEEAPLGGEEDVRAAV
                     EAAGEAFADWSAKSPRARAKILFFAAEEVRRRNTDLAALLTAEQGKPIREAVDEINGF
                     ANILEYYYALSAGERGEFANLPGYGHTIVLRRPLGVCGAIIPWNMPAIIMGWKIGPAL
                     TTGNTMVLKPATTAPLTCGRLASILEGAGLPPGVLNVVTGPGETVGREIARNPEIRKV
                     SFTGEAGTGRQVALDAAPALKRVTLELGGSDPMIVCDDADIPMAVEGAVRGRFYNCGQ
                     TCTAVKRLYVFDSIADEFIRRLRSRVESIVVGNGMDRGVDMGPLNNPAGLERVVREVD
                     AVCEHRDGEIVTGGQAPRGEGYERGFFFLPTLVAGLSHDSPLFSEEVFGPVLPVATVA
                     DLDEALIRANDSRYGLGASVWTRNADLIARATEGLEAGIVWVNQHLKIPPEVPFGGTK
                     GSGIGRENGSRALEAYTEEKVVMIRL"
     gene            complement(1905..2990)
                     /locus_tag="PLI76_09710"
     CDS             complement(1905..2990)
                     /locus_tag="PLI76_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI62349.1"
                     /translation="MPRSLPVLPGIFVVMALSNAVVPVLPGFAEGATMQGAVYAAYFL
                     GAFIMVLPAGMASDHLGRVPLIHAGLLLTVASGALILLFPSGLPLLLFRAVEGIGAGL
                     FLSAALAWANSHPSAGRVSGYVMAAQNLGLVAGLLAAGWLDAVFDSHLAGIAFFTALA
                     IPALVMSAAIRESNHAGFVEADLSLIARNYFWLFTTAAVLVGMTGVVAALYPGYTGGD
                     PSLLALQLATVNIATVIAVLVVPRLDLRPAPAIRASSLLMAGAVAASYATPYALPVVG
                     ALAGVIIVAVLAFLAETGIRQGVMIGLFNTATYAGFTVLPALAGAIAGEFGFLAGFLL
                     PAGLAAAMAATIGRCPAQQSAYNRPHP"
     gene            complement(2993..>4701)
                     /locus_tag="PLI76_09715"
     CDS             complement(2993..>4701)
                     /locus_tag="PLI76_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-CASP ribonuclease aCPSF1"
                     /protein_id="HOI62350.1"
                     /translation="VVRPNILEDPERAAQEIRSVVPENAGISDLFFDPETGEVLIEAE
                     KPGVVIGKNGITLREITKQIGWTPKVVRTPPIESTTVKQIRTFLRSVKDERKAFLRKI
                     GHRIHREVTSKDQWLRVTTLGCCREVGRAAFLISTPESKILVDCGEKPGSATNGAPYL
                     YVPEIYPLDSLDAVVLTHAHLDHCALVPLLFKYGYEGPVYSTPPTRDLSTMLLLDYLD
                     VVRKETDKIPYTSNEVRTYMKHSIALNYGNVTDIAPDVKLTFHNAGHILGSAIAHFHI
                     GEGLYNIAFTGDFNYQKTRLFSPAVSSFPRLEALFMESTYGGANDIQPQRKDAEEKLY
                     ETVSTTIKRGGKVIIPAFAVGRSQEVMLALEEGIRKQKIPPVKIYLDGMIKEATAIHT
                     TYPEYLNSDLRNQIFREGLNPFLADAFVQVDSPDLREKVIGGDPCVIITTSGMLNGGP
                     VMEYLRTLGPDERNSLIFVGYQAEGTLGRRIQKGWREIPLGWRETIVINLEIATSDGF
                     SGHSDRKQLMNYVAHLQPRPEKIFTIHGDENKTIDLASSIYKRHRIETHSPLNLETYR
                     MI"
BASE COUNT          851 a         1545 c         1465 g          840 t
ORIGIN      
        1 cggtcatgtt tccggttgtg ttcccggtca cgtttccggg cgtcatggtt gcattcccat
       61 ccccgaccag cgccgccgtc acggcgaacg ggatggccag aaggatgagg atgcacacgc
      121 atggagctat ccatcgtttc atgtcacttt ccccccttgg gagcgctaag ggcatgcatg
      181 taattatata taaatactgt tatcgttccg gcgacacctc caatagcaga acaaccgcgt
      241 aacgaactct cttttctctc gtagatcccc ggggagaggg ggcgaatccc cgggctcgcg
      301 caccccttcg cggccatcac ggcaacgtgc gcatgagccg gcagggtatg agcgatgagt
      361 gaacctctcg cgccggatca tctcctacac ccgggacccg cgcatgaaga cagcccggtg
      421 cagcagggcc cgggtggatg agaggccgcc ccggagggcc ccgttcacaa ccgtatcatc
      481 accaccttct cctccgtata cgcttcaagc gcccggctgc cgttctccct cccgatacca
      541 ctccccttcg tcccgccaaa cggcacctcc ggggggatct tcaggtgctg gttgacccag
      601 acgatccccg cctcaagccc ctccgtcgcc cgggcgatca gatccgcatt ccgggtccag
      661 accgacgcac cgaggccgta acggctgtcg tttgcccgta taagcgcctc gtcgaggtcc
      721 gcgacggtcg cgaccgggag caccggacca aagacctcct cggagaagag cggggagtcg
      781 tgcgaaaggc cggcaacaag cgtcggcagg aagaagaacc cccgctcgta cccctctccg
      841 cggggagcct gcccgcccgt gacgatctcc ccgtcccggt gttcacaaac cgcatccacc
      901 tcgcgcacca cccgctcaag ccctgcagga ttgttcaacg gacccatatc gacgccgcgg
      961 tccatgccgt tcccgaccac gatcgactcg acccttgatc tcaggcgccg gataaactcg
     1021 tccgcaatcg agtcgaagac gtagagccgt tttaccgccg tgcaggtctg cccgcagttg
     1081 tagaaccgtc ccctgaccgc cccctcgacc gccatcggta tatcggcatc gtcgcagacg
     1141 atcataggat cgctcccccc gagctccagc gtgacccgtt tcaatgccgg cgcggcatcc
     1201 agggcgacct gcctcccggt cccggcctcg ccggtgaacg agaccttccg gatctccgga
     1261 tttcgcgcaa tctcccggcc gaccgtctcc cccggacccg tgacgacgtt caggacgccc
     1321 ggtgggagcc cggcgccctc gaggatcgac gcaagccttc cgcaggtcag cggggccgtc
     1381 gtcgcgggtt tcaggaccat ggtgtttccc gtcgtgaggg ccggcccgat cttccagccc
     1441 ataatgatcg ccggcatgtt ccagggtatg atcgcaccgc atacgccaag agggcgccgg
     1501 agaacgatgg tatgcccgta gcccgggagg tttgcgaact ctccccgctc ccctgcggag
     1561 agagcgtaat aatactcgag tatgtttgca aacccgttga tctcgtcgac cgcctcccgg
     1621 atgggtttcc cctgctcggc cgtcaggaga gccgcaaggt cggtgttcct gcggcgcacc
     1681 tcctctgcgg cgaagaagag gatcttcgcc cgggctctcg ggctcttcgc cgaccagtct
     1741 gcaaacgcct ccccggccgc ctcgacagcc gccctgacat cctcctcgcc gccaagcggc
     1801 gcctcctcga gcatatcccc cgtcgcaggg ttacgcaccg caaacgcctt cccggaggcc
     1861 gcatcgcgcc actcgccgtt gatcaacagt ttcatacgat gatcctatgg atggggccgg
     1921 ttatatgcgc tctgttgggc gggacatctg ccgatcgtgg ccgccatcgc cgccgccagc
     1981 ccggcaggca ggaggaaccc ggcgaggaac ccgaactccc ctgcgatcgc cccggcaaga
     2041 gcggggagga ccgtaaaccc ggcataggtc gcggtgttga agagcccgat cataacgccc
     2101 tgccggatcc cggtctctgc aaggaacgcg agcaccgcga cgatgatcac cccggcaaga
     2161 gctccgacga ccgggagggc atacggtgtt gcgtaactcg ccgcgaccgc cccggccatg
     2221 aggagcgaag aggcccggat ggccggggcg ggacggagat cgaggcgggg aacgacgagc
     2281 acggcgatga cggtcgcgat gttgaccgtc gcgagctgga gggcaagaag cgacggatcg
     2341 cctccggtgt acccggggta gagcgccgcc accacccccg tcatccctac gaggacggct
     2401 gccgtggtaa agagccagaa gtagttccgg gcgatgagcg agagatcggc ctctacaaac
     2461 ccggcgtggt tgctctccct gatggcggcg ctcatcacga gcgcagggat ggcaagcgcc
     2521 gtgaagaaag cgatcccggc aagatggctg tcgaagacgg catcgagcca ccccgcggcg
     2581 agaagtcccg cgaccaggcc gaggttctgg gctgccatga cgtatccgct gacccggccg
     2641 gccgacgggt gcgagtttgc ccatgcaagc gccgccgaga ggaagaggcc ggccccgatc
     2701 ccctcgaccg cccggaagag gaggagcggg aggcctgaag ggaagagcaa aatgagagcc
     2761 ccgcttgcaa ccgtcaggag aaggccggcg tggatgagcg gcacgcggcc gaggtggtcg
     2821 cttgccatcc cggccggcag caccatgatg aacgcgccca aaaaatatgc ggcgtagacc
     2881 gcgccctgca tagttgcccc ctccgcaaaa cccgggagca ccggaacgac ggcgtttgag
     2941 agcgccatca ccacgaatat accggggaga acgggcaggc tccgcggcat cctcagatca
     3001 tgcggtaggt ctcgaggttc aggggagaat gcgtctcgat acggtgccgc ttgtagatag
     3061 aactcgcaag atcgatcgtc ttgttctcgt cgccgtggat ggtgaatatc ttctccgggc
     3121 ggggctggag atgagcgacg tagttcatca gctgctttcg gtccgagtga ccggagaatc
     3181 cgtcgctcgt cgcgatctcg aggttgatca cgatcgtctc gcgccacccg agcgggatct
     3241 cccgccaccc tttctggata cgccgcccga gcgtcccttc cgcctggtac ccgacgaaga
     3301 tgagcgagtt gcgttcatcg gggccgaggg tcctgaggta ctccatcacc ggcccgccgt
     3361 tcaacatacc gctcgtcgtg atgatgacgc aggggtcgcc cccgatcacc ttctcgcgga
     3421 ggtccggcga gtctacctgg acaaaggcat cggcaaggaa ggggttcaac ccctcgcgga
     3481 agatctggtt tcggagatcg ctgttgaggt actccgggta ggtcgtgtgg atcgccgtcg
     3541 cctccttgat catgccgtcg agatagatct tcaccggcgg tatcttctgc ttccggatcc
     3601 cctcctcgag ggcgagcatg acctcctgcg accgcccgac ggcgaacgcc ggtatgatca
     3661 ccttgccgcc gcgtttgatc gtcgtcgaga ccgtctcgta gagtttctct tctgcgtcct
     3721 ttctctgcgg ctggatatcg tttgctccac cgtaggtgct ctccatgaag agcgcctcga
     3781 ggcgggggaa cgacgacacc gcgggagaaa agagcctcgt cttctggtag ttgaagtctc
     3841 cggtgaatgc gatgttgtag agaccctctc cgatatggaa gtgtgcgatg gccgacccga
     3901 ggatatgccc cgcgttatgg aaggtgagct tgacatcggg ggcgatgtcg gtcacgttgc
     3961 cgtagttgag ggcgatggag tgcttcatgt aggtccggac ctcgtttgag gtatagggga
     4021 tcttgtccgt ctccttccgg acgacgtcca ggtagtcgag caggagcatc gtcgagaggt
     4081 ccctcgtcgg cggggtcgag tagaccggcc cctcgtagcc gtacttgaag aggagaggga
     4141 cgagagcaca gtggtcgagg tgcgcgtggg tgagcaccac cgcgtcgagc gagtcaagcg
     4201 gatagatctc gggcacgtaa agatacggcg ccccgttcgt ggcactgccg ggcttctcgc
     4261 cgcaatccac caggatcttg ctctccggcg tcgatatcag aaacgcggcc cggccgacct
     4321 cgcggcagca cccgagcgtc gtgacccgca gccactggtc cttgctcgtg acctcgcgat
     4381 ggatgcgatg gccgatcttg cgcaggaacg ccttgcgctc gtccttcacc gaccggagga
     4441 acgtgcggat ctgcttcacc gtcgtgctct cgatgggcgg tgtccggaca accttcggtg
     4501 tccagccgat ctgttttgtg atctcgcgaa gcgtgatgcc gttcttgccg atgaccacgc
     4561 cgggcttctc tgcctcgatc aggacctcgc ccgtctccgg gtcaaagaag agatcggata
     4621 tcccggcgtt ctccggcaca acggagcgga tctcctgggc cgcccgttcc ggatcttcga
     4681 ggatgttcgg gcgcacgacg a
//
LOCUS       DAOSEE010000153         4691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_6444_length_4691_cov_229.921700, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000153 DAOSEE010000000
VERSION     DAOSEE010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4691
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6444_length_4691_cov_229.921700"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /locus_tag="PLI76_09720"
     CDS             complement(<1..173)
                     /locus_tag="PLI76_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62351.1"
                     /translation="MTETNGKLQYVPTTCPYCGVGCSLNLVVNDGKLVGVEANKRSPI
                     NEGKLCPKGATCW"
     gene            496..1137
                     /locus_tag="PLI76_09725"
     CDS             496..1137
                     /locus_tag="PLI76_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HOI62352.1"
                     /translation="MQPRLNHTWDDIEARLRSKGSSPEMIEHFRRCIDFHTFAAPGLL
                     VGVFMVDYALELLNPPSGEKIYAVCETTKCLPDALQVVAHSTTGNHRLRVIPIGKFAI
                     TVNRPAETPLVDGIRVFVDGEKIGRYPTFALWYTKDPLFDPRTRGAALLDEIVDAGRD
                     LLSYERVRVKVPQKWPWKAAVCTICGEMVPDNLLIDGACADCRSQSYYEKMPY"
     gene            1212..2024
                     /gene="tsaA"
                     /locus_tag="PLI76_09730"
     CDS             1212..2024
                     /gene="tsaA"
                     /locus_tag="PLI76_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HOI62353.1"
                     /translation="MEIFPIGIVHSSIRSRSDMPVQGVDAEIEVFPDYVAALDGIKES
                     SHLILICWLHEADRNVLKAVARKVSGDLPERGVFSLRSPVRPNPLSVSVVRLCGLREG
                     RILNLANVDLIDETPVIDIKPYQRGWDCIFSATGHDRTEKIRKMGPGEYRKSLIREAV
                     NYHGELCPGVAVAVRIAEAAARILGCDLAVPEVAVAPGTDPCIADALIGITGARPGNR
                     RLRHPGGEDCIVAAPGMEVIFHLRDAPESIEAVLAAREESLFDCELAFPDDR"
     gene            2107..3933
                     /locus_tag="PLI76_09735"
     CDS             2107..3933
                     /locus_tag="PLI76_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HOI62354.1"
                     /translation="MSRRYLNLTPLPEALAAMRRAFPPSDHVETVPLCLAVGRVTAEP
                     LYAEYSVPEVEIAKFDGYAVKSRDTPGAQDQRPLPLREYARINTGEILPPSFDAVVMV
                     EDTWDDGGTPWIRKSAAPWQNVRHAGEDIRAGELALPKGHRIRPFDLGALATYGITRL
                     AVRSVRIGIIPTGNDLVPLGVVPAPGQTIETNTLMAEAYLTRMGATCRRYEIVPDDPD
                     LIRSALETALDANDLVILSAGSSAGSRDFSRDVVGALGEVIFHGIAVRPGKPVLLASV
                     GGKPVLGMPGYPVAAQTILREVAGSLLAWWGLPALPEGELDVRLSRRLASDLGFDEFV
                     PVSVGRVNGTCFATPHPRGGGIQMAVVRANGYIHIPAEREGFEAGEEVRVRLTVSPAE
                     IARTLICVGVRDPTITELGNCLADAGYLLHCCKASTMGAVLALRAKTCHAASISLPET
                     EAAWNDQVLRYLPGVDLLRVQVARTELGLASAGDLDAGNPASFRFINQPKGTAARVFL
                     DAWLAREGIDAGIVTGYGHEVRTPAAVAAAVGNGFADAGVCPAAMAREAGLRFTPLGY
                     ETRYLLVRKELAADEGVASLVRAARSPEFEEHLRSRERCRAG"
     gene            4165..4593
                     /locus_tag="PLI76_09740"
     CDS             4165..4593
                     /locus_tag="PLI76_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOI62355.1"
                     /translation="MPVSEQTQSQIMAVLRRMADAMGRKDIDGLVALVDPEFRAFGTG
                     PDEKAIGREAYRRHLERDFAQAEKVAIDLSDIRIGAEGTVAWVMADMAYHIVVGGVPQ
                     TLNGRMTAVLRGTGHAWIFVQMHYSLPAAGQEAGQSYPAV"
BASE COUNT          856 a         1446 c         1478 g          911 t
ORIGIN      
        1 tcccagcagg ttgctccctt gggacagagt ttaccctcgt tgatcgggct cctcttgttg
       61 gcttctactc ccacaagttt gccatcgttc accacgaggt taaggctgca ccctacacca
      121 cagtaggggc aggtcgtggg aacgtactgt aactttccat tggtttctgt cattcacatt
      181 ctcccgattg ttgtgttcaa cgtcggttgc caagcatgtc aggaaaggcg gacatgcaca
      241 cagagttgtt ttactctact aagtagtata taaatttaaa cttacccttt taacatttgt
      301 ctgtatacaa tcgaatatat ttgatcctga tctgacatga gctcaaccat ttgatatatt
      361 gcaccatacg ttgactgtat atcgattaga aatgattttt gctttattat gccctgaatg
      421 tgctttcaag atgtcaagtt aatttaattt aatgatccaa aatgtttaca atcaacctta
      481 accaaacatt cttccgtgca accgagactg aatcatacat gggatgatat cgaggccaga
      541 ctcaggtcga aaggctcctc tccggagatg atcgagcact tccggcgatg catcgatttt
      601 catacattcg cagcccccgg gctcttggtc ggggtcttca tggtggacta cgccctggaa
      661 cttctcaacc ctccttctgg cgagaagatc tacgccgtct gcgagaccac gaagtgtctg
      721 cccgacgctc tccaggtggt tgcccatagt acgaccggta accaccgcct cagggtgatc
      781 ccgatcggaa agtttgccat caccgtgaac agaccggccg agacaccact ggtggatgga
      841 atccgggtct tcgtcgacgg cgagaagata gggcggtatc cgacatttgc cctgtggtac
      901 accaaagacc ctctcttcga cccgcgaact cggggcgctg cgcttcttga cgagattgtt
      961 gatgccgggc gcgacctcct ctcgtacgaa cgcgtgcggg tgaaggtacc gcagaagtgg
     1021 ccctggaaag cggccgtctg caccatctgc ggtgagatgg tccctgacaa cctgcttatc
     1081 gacggagcct gtgccgactg ccggtctcag tcttactacg agaagatgcc ctactgacgg
     1141 aggaaacccc tccctcttcc cccgtggaaa gggtctatga gggtactggt tcattgtcac
     1201 agatgcagcg catggagatc ttccctatcg ggatcgtgca ctcgagcatc cgctcccgga
     1261 gcgatatgcc ggtccagggc gtcgatgccg agatcgaggt cttccccgac tacgtagcag
     1321 cgctggacgg catcaaagag agctcgcacc tgatcctgat ctgctggctc cacgaggcgg
     1381 accggaacgt cctgaaggcc gtggcccgga aggtctcagg cgacctcccg gagaggggtg
     1441 tcttctccct ccggtctccg gtgcggccga accccctctc ggtgtcggtg gtgcggctat
     1501 gcggtctccg tgaagggcgg atcctcaacc tcgcgaacgt cgacctgatc gacgaaacac
     1561 cggtgatcga catcaaaccc taccagaggg gatgggactg tatcttctcc gcgaccggcc
     1621 acgaccggac tgagaagata cggaagatgg ggcccggaga gtaccgaaaa agcctcattc
     1681 gcgaggccgt gaactaccac ggggagctct gtccgggcgt cgcggtcgcg gtgaggatcg
     1741 cggaggcggc ggcgcgcatt ctcgggtgcg acctcgctgt gccagaggtc gcggtcgcgc
     1801 ccggcaccga tccctgcatc gccgacgccc tcatcggaat caccggcgcg cgcccgggaa
     1861 accgccggct gaggcatccg ggtggagaag actgcatcgt tgccgcgccc gggatggagg
     1921 tgatcttcca cctccgggat gccccggaga gcatcgaggc cgtccttgca gcgcgcgagg
     1981 agtcgctctt cgactgtgag cttgccttcc cggatgaccg ataggtaaac caggagtaat
     2041 ggagaatcgt atgtcacggg cgggggtaaa aaccctccac cggcacgcta taagggagtc
     2101 atgagtatgt cacgccgata tctgaacctt accccgcttc ccgaagcgct cgcggcgatg
     2161 cggcgggcgt ttcctccgtc ggatcatgtc gagaccgtgc cgctctgcct tgccgtcggc
     2221 agggtgacgg ccgaaccgct gtacgcagag tactctgttc ccgaggtcga gatcgcgaag
     2281 ttcgacggtt atgcggtgaa gagcagggat accccggggg cgcaggacca gcggccgctg
     2341 cctctccgcg agtatgcccg catcaacacc ggtgagatac tccccccctc gtttgacgcc
     2401 gtcgtcatgg tcgaggatac ctgggacgac ggcgggacac cctggatcag gaagtctgcc
     2461 gcgccctggc agaacgtccg gcatgccggg gaggatatcc gtgcaggcga acttgccctc
     2521 ccgaaaggcc accggatccg gccgttcgat ctcggcgcgc tcgcaaccta cgggataacc
     2581 cggctcgctg tccggtcggt ccggatcggc atcataccga caggaaacga cctcgtcccg
     2641 ctcggcgtgg tgcccgcccc gggtcagacg atcgagacga acaccctcat ggcggaggcg
     2701 tacctcaccc ggatgggggc gacctgccgc cgttatgaga tcgttcccga cgatccggat
     2761 ctgattcgga gcgcgcttga gacggctctc gacgcaaacg atctcgtcat cctctcggca
     2821 ggctcgtcgg ccggttcccg ggacttctcc cgcgacgtcg tcggcgcgct cggggaggtg
     2881 atcttccacg gcatcgcagt ccggccggga aaaccggtgc tgctcgcgag cgtcggcggc
     2941 aaaccggtcc tcggtatgcc gggctatccg gtcgccgcgc agaccatcct ccgggaggtt
     3001 gccgggagtc tgctcgcgtg gtgggggctc ccggcgctcc cggaggggga actggatgtc
     3061 cggctgtcaa ggaggctggc atccgatctc gggtttgacg agttcgtccc ggtctcggtc
     3121 gggcgggtga acggcacctg ttttgcgacc cctcatcccc gggggggcgg catccagatg
     3181 gcggtggtcc gggcgaacgg ctacatccat atacccgccg agcgcgaggg gttcgaggcc
     3241 ggggaggagg tgcgggtccg gctcaccgtc tccccggctg aaatcgcccg cacgctcatc
     3301 tgtgtcggtg tgcgcgaccc caccatcacc gagctcggca actgccttgc ggatgccggt
     3361 tacctgctcc actgctgtaa agcctcgacg atgggggcgg tgctcgctct gcgggcgaag
     3421 acctgccacg ccgcgtcgat ctctcttccg gagacggaag cggcctggaa cgatcaagtg
     3481 ctccggtacc tgcccggggt cgatctcctg cgggtgcagg tggcccggac ggagctcgga
     3541 ctcgcctcgg ccggcgacct ggatgccggc aaccctgcgt cgttccggtt catcaaccag
     3601 ccgaaaggca cggcagcgcg ggtcttcctc gatgcctggc tggcccggga aggcatcgat
     3661 gcgggcatcg tcaccgggta cgggcacgag gtgaggacgc ctgcggccgt cgccgcagcc
     3721 gtcggcaatg ggtttgccga cgcaggcgtc tgtccggcag ccatggcgcg ggaggcgggg
     3781 ctccggttca ccccgctcgg ctacgagacg cgttacctcc tggtccggaa ggaacttgcc
     3841 gcggacgagg gtgttgcaag cctcgtccgg gccgcccggt cgccggagtt cgaagagcac
     3901 ctccggtcgc gggagagatg ccgggcaggg taaccggata ccctgcccgg agggacttaa
     3961 aggcacgtgg ctacaggagg ttcatcggct gccgcatcca gagcgcaaca cgcctgaaca
     4021 ggccagaagc tgcccggcaa cccgccggct atctcctgtg cccgttgtgg ataaccgtgg
     4081 tgcataagcc atccgttatc gaccgtcccc gggttggcgc gccggtccga aaagatcatc
     4141 tcctatcggg agagagagtt cttcatgcca gtcagcgaac agacacagag ccagatcatg
     4201 gcagtgctcc ggcggatggc ggatgccatg ggcaggaagg acatcgacgg cctggttgcg
     4261 ctcgtcgatc ctgagttccg ggcattcggg accggtcccg acgagaaggc gatcgggagg
     4321 gaggcctacc gccggcacct tgaacgcgac tttgcgcagg cggagaaggt cgcgatcgac
     4381 ctctccgaca tccgcatcgg tgccgaagga acggtcgcct gggtcatggc cgatatggca
     4441 taccatatcg tcgtcggcgg cgtcccgcaa accctgaatg ggcggatgac ggcggtcctc
     4501 cgggggacag gccatgcgtg gatctttgtc cagatgcact actctctccc ggcggcgggc
     4561 caggaggccg gacagtcgta tccggcggta taattttttg cccgccgagg gcactgtgcc
     4621 ggtttcagcg ctcgttatag ggaataccga tacgttctcc tccggggcac ccccctactc
     4681 tggtggcggg g
//
LOCUS       DAOSEE010000154         4681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_6461_length_4681_cov_200.661695, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000154 DAOSEE010000000
VERSION     DAOSEE010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4681
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6461_length_4681_cov_200.661695"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PLI76_09745"
     CDS             <1..447
                     /locus_tag="PLI76_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOI62356.1"
                     /translation="MGGVPGSKIVQFDMGNLTQDFPVELSIVVEETCQIRHTALEAAR
                     IGINRQLLKEVGRANYHLKIRTFPHHVLRENKQATGAGADRVSEGMRLAFGKAVGTAA
                     RVQSGQKVFSVWTTPQYAEKAKVSLKRGIYKIPTPARIVEEQTMEA"
     gene            553..1389
                     /locus_tag="PLI76_09750"
     CDS             553..1389
                     /locus_tag="PLI76_09750"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOI62357.1"
                     /translation="MTHPADPALLRALATATEKALLEAEIRLPTDVLAALRRAAAAER
                     NEVARQEFALILENIALAGERQVPICQDTGVPVVYLTLPPDVPFTPDLFAGIREGVRR
                     ATTAIPLRPNVVDPLTRENTGDNTGAGMPAVHVVFGDRLTVTVLPKGAGSENASRIGM
                     LLPSQAADIPRFVAETMLIAGGKPCPPVFLGVGIGGTFDGAAALAKEALLLPVDTMDD
                     YEQEICDAVNALGIGPMGLGGETTALAVKVKRRDCHTASLPVAVNVQCWACRRATVEV
                     ER"
     gene            1356..1928
                     /locus_tag="PLI76_09755"
     CDS             1356..1928
                     /locus_tag="PLI76_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HOI62358.1"
                     /translation="MPPGYRGGGAVNLTTPLGDEVLDLRAGDRVTLTGTIYTARDEAH
                     LRMMEEGIPFDPVGAAVYHCGPVVRDGRLVVAGPTTSARMNALTGFLIDAGVRALIGK
                     GGMGPEVVEQLRGRAVYLAFTGGCAALAAARMSLSGVYFEDLGMAEAVWIIEADHLPL
                     TVGIDARGGDLFSAVREKAKTQFHQRFNIR"
     gene            1940..2212
                     /locus_tag="PLI76_09760"
     CDS             1940..2212
                     /locus_tag="PLI76_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOI62359.1"
                     /translation="MKLVIDESRCKGCNLCVLVCPYGIFREGTELNSRGIVSPVLDRP
                     ERCTNCRLQALYGRMLCGICHMICPDQAISWVEEKPFEPHKVEVEF"
     gene            2209..3309
                     /locus_tag="PLI76_09765"
     CDS             2209..3309
                     /locus_tag="PLI76_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOI62360.1"
                     /translation="MSRVEFMQGNAACAEGALAAGCRFFGGYPITPSTEIAEAMARRL
                     PKAGGVFISMEDELASIAAVIGAAWTGVRAMTATSGPGFSLMMENIGYAAMTETPCVI
                     VNVQRGGPSTGQPTRAAQGDMLQCRFGSHGDYSIIALTPNSVQETFDLTAKAFDLADR
                     FRVPTFLMTDEIVGHMRERVTIPDAVDITRSSPLAESSLPFEAGDDGIPGFAPFGSGR
                     AVHVTGLTHDERGYPDTTDPAAHDRLVRRLVAKVESARRDIADYEVTNPDAGTVFVTY
                     GPPSRTVEQVMRDYPDDAVGHLRLRVVWPFPEFALHEFPNAETYIVPELNMGQVVREV
                     ERHVDRPVVPLGKIGGELHTPAELVRALEAHR"
     gene            3306..4094
                     /locus_tag="PLI76_09770"
     CDS             3306..4094
                     /locus_tag="PLI76_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOI62361.1"
                     /translation="MIAPDWLREDRLPHIYCTGCGNGTVINCTLAAVEEMGWKRDETV
                     FVSGIGCSSRAPGYILTDSLHTTHGRALAFATGVKMARPEFNVVVFTGDGDLAAIGGN
                     HFIHACRRNVDMTVVCMNNHIYGMTGGQGSPTTPPGAISTTTPYGMSEPAFDLAELAV
                     AAGANYVARWTSYHVKELTKAIAAGMRTPGFAFIEARTQCPTNYGRHNKLRTVPAMIE
                     HIRSHAMLIEKRDRLVREGRPVPEDIFTIGELVRRNRPAMGVRR"
     gene            4091..4630
                     /locus_tag="PLI76_09775"
     CDS             4091..4630
                     /locus_tag="PLI76_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOI62362.1"
                     /translation="MRHEVRFSGYGGQGIILSAVILGRAAALYDGKHAVQTQVYGPEA
                     RGGASMGQVVIDDRPILYPEVADPDIYAIMSQQGFEKYGVRARPDAVMLLDTDLVRSR
                     PGCRYYEIPATAEAKTVLGREIVANIVMIGALVTSTGIVSREAIERAVLDSVPKGTEN
                     LNMKALKRGFELGERVCNL"
BASE COUNT          852 a         1586 c         1535 g          708 t
ORIGIN      
        1 atgggcggcg tgccgggcag caagatcgtg cagtttgata tgggcaacct cacccaggac
       61 ttcccggtcg aactctccat cgtcgtcgaa gagacctgcc agatacgcca cacggccctc
      121 gaggccgccc gtatcggcat caaccggcag ctcttgaagg aggtcgggag ggcaaactat
      181 cacttgaaga tacggacctt cccccaccac gtcctccgcg agaacaagca ggcgaccggc
      241 gcaggagccg accgtgtctc cgaaggcatg cgtcttgcct tcggaaaggc cgtcggcacc
      301 gcggcgcggg tccagagcgg ccagaaagtc ttctccgtct ggacaacccc ccagtacgcc
      361 gagaaggcga aggtctctct caaacgcggc atctacaaga taccgacccc cgcacggatc
      421 gtcgaagagc agaccatgga agcgtaggcc tgcacaacgc ccaattcttt tttgcgccga
      481 acggttcgca gagggcggaa ctgaggccgg cgggaataga gtatttatag cctcttccca
      541 ccagtaataa taatgactca tccggcagac ccggcgctcc tgcgcgcgct cgccaccgcc
      601 accgagaagg ctcttctaga ggccgagatc cggctcccca cggacgtcct cgcggcgctc
      661 cggagggcgg ccgcggccga gaggaacgag gtcgcccggc aggaattcgc ccttatcctc
      721 gaaaacatcg ctctcgccgg agagcggcag gtgccgatct gccaggacac cggcgtgccg
      781 gtggtctacc tcaccctccc gcccgacgtt ccgttcacgc ccgacctttt cgcgggaatc
      841 cgggaaggag tgcgccgggc aaccaccgcg atcccgctcc gcccgaacgt cgtcgatccc
      901 ctgacccggg agaacaccgg cgacaacacc ggggccggca tgcccgcggt tcacgtcgtt
      961 ttcggcgacc ggctcacggt gacggtcctc ccgaagggcg cgggctctga gaacgcatcc
     1021 aggatcggga tgctcctccc ctcgcaggca gcggatatcc cccggttcgt cgcggagacg
     1081 atgctcatcg cgggcggcaa accctgcccg ccggtcttcc tcggcgtcgg tatcggcggg
     1141 acgtttgacg gggccgcggc gctcgcaaag gaggccctcc tcctcccggt cgacaccatg
     1201 gacgactacg agcaggagat ctgcgacgcg gtcaacgccc tcggcatagg gccgatgggt
     1261 ctagggggcg agacgacggc gcttgccgtg aaggtgaagc gcagagactg ccacaccgca
     1321 tccctcccgg tggcggtgaa cgtccagtgc tgggcatgcc gccgggctac cgtggaggtg
     1381 gagcggtgaa ccttaccaca ccgctcggcg acgaggtcct cgacctccgg gcgggcgacc
     1441 gggtcaccct cacgggaacg atctacaccg cacgggacga ggcccacctc cgcatgatgg
     1501 aagaaggcat ccccttcgac cccgtgggcg ccgcggtcta ccactgcggc ccggtggtcc
     1561 gggacggccg tctcgtcgtc gccggaccca ccacatccgc gcggatgaac gctctcacgg
     1621 ggttcctcat cgacgcgggc gtccgcgccc tcatcggcaa ggggggaatg gggccggagg
     1681 tggtcgagca gctccggggg cgcgccgtct acctcgcgtt cacgggaggc tgcgcagccc
     1741 ttgccgccgc ccggatgagc ctctccggcg tctattttga ggacctcggc atggccgaag
     1801 cggtctggat catcgaggcg gatcacctcc cgctgacggt cggcatcgac gcccgcggcg
     1861 gcgatctctt cagtgcagta cgcgagaaag cgaaaacaca atttcaccag cgcttcaata
     1921 tcaggtagga taccgtctca tgaaacttgt catcgatgaa agccgctgca aggggtgcaa
     1981 cctctgcgtg ctggtctgcc cctacgggat ctttcgagaa gggacggaac tcaacagccg
     2041 ggggatcgtc tcccccgtcc tcgaccgccc cgagcggtgc acgaactgcc ggctgcaggc
     2101 tctctacggg cggatgctct gcgggatctg ccacatgatc tgccccgacc aggccatctc
     2161 ctgggtcgag gagaagccgt ttgagccgca caaggtggag gtggagtttt gagcagggtt
     2221 gagttcatgc aggggaacgc cgcctgcgcg gagggggcgc ttgctgccgg gtgccggttc
     2281 ttcggcggct acccgatcac gccctcgacc gagatcgccg aagcgatggc ccggcggctc
     2341 ccgaaggccg gcggggtctt catatccatg gaggacgaac tcgcgagcat cgccgcggtc
     2401 atcggcgccg cctggacggg cgtccgggcc atgaccgcga cgagcggccc ggggttctct
     2461 ctgatgatgg agaacatagg ctacgcggcc atgaccgaga cgccgtgcgt catcgtcaac
     2521 gtccagcgag gcggcccgag caccggccag ccgacgcggg cggcgcaggg cgacatgctc
     2581 cagtgccggt tcgggtcgca cggcgactac agcatcatag cgctcacgcc gaactccgtc
     2641 caggagacat tcgacctgac ggcgaaggcg ttcgatctcg ccgaccggtt cagggtcccc
     2701 accttcctga tgaccgacga gatcgtcggc cacatgcggg agcgggtgac catccccgat
     2761 gcggtcgata tcacccgctc ctccccgctc gcggaaagct ctctaccgtt cgaggccggc
     2821 gacgacggga tacccgggtt tgcgcccttc ggctcgggga gggcggtcca cgtgaccggg
     2881 ctcacccacg acgagcgcgg ctacccggac acgaccgatc ccgcggcgca cgaccgactg
     2941 gtgaggaggc tcgtcgcgaa ggtcgagtcg gcgcggcgcg atatcgccga ctacgaggtc
     3001 acgaaccccg acgccgggac ggtcttcgtc acctacggcc cgccgtcccg gacggtcgaa
     3061 caggtgatgc gcgattaccc cgacgacgcc gtcggccacc tccggctcag ggtcgtctgg
     3121 ccgttcccgg agttcgcgct ccacgagttc ccgaacgcag agacctatat cgtgccggag
     3181 ctgaacatgg gccaggtggt ccgggaggtc gagcgccacg tcgaccggcc ggtcgtcccc
     3241 ctcggaaaga tcggcggcga actccacacc cccgccgaac tcgtccgggc cctggaggcg
     3301 caccgatgat cgcgcccgac tggctcaggg aggaccgcct gccccacata tactgcaccg
     3361 ggtgcggcaa cgggacggtc atcaactgca ccctcgcggc ggtggaggag atgggctgga
     3421 aacgggacga gacggtcttc gtctccggta tcgggtgctc gtcccgcgcc cccggctaca
     3481 tactcaccga ctcgctgcac accacccacg gccgggcgct cgcgttcgct acgggtgtga
     3541 agatggcgcg gccggagttc aacgtcgtcg tcttcacggg cgacggcgac cttgccgcca
     3601 tcggcggcaa ccacttcatc cacgcctgcc gccggaacgt ggacatgacg gtggtctgca
     3661 tgaacaacca tatctacggc atgaccggcg gccaggggag cccgaccacc ccgccggggg
     3721 ccatatcgac gacgacgccc tacggcatga gcgaaccggc cttcgatctt gcggaactcg
     3781 cggtcgccgc cggcgcgaac tacgtcgccc gctggacgtc ctatcacgtc aaagagctca
     3841 cgaaggccat cgccgccggc atgcggacgc cggggtttgc gttcatcgag gcaaggaccc
     3901 agtgcccgac gaactacggc cgccacaaca agctcaggac cgtcccggcc atgatcgaac
     3961 acatccggag ccacgcgatg ctcatcgaga agcgcgaccg cctggtccga gagggaagac
     4021 cggtcccgga ggatatcttc accatcggcg aactcgtccg gcggaaccgg ccggcgatgg
     4081 gggtgcggcg atgaggcacg aggtcaggtt ctcgggatac ggcggccagg ggatcatcct
     4141 ctccgcggtc atactcgggc gggcggcggc gctctacgac gggaagcacg ccgtccagac
     4201 acaggtctac ggcccggagg cccggggcgg ggcatcgatg ggacaggtgg tgatcgacga
     4261 tcgcccgatc ctctaccccg aggtggccga ccccgacata tacgccatca tgtcgcagca
     4321 ggggtttgag aagtacgggg tcagggcccg gccggacgcg gtcatgctcc tggataccga
     4381 tctcgtccgg tcccgcccgg gatgccgcta ctatgagata ccggcgacgg cggaggcaaa
     4441 gaccgtcctc ggccgggaga tcgtcgcaaa catcgtcatg atcggcgccc tcgtgacctc
     4501 gaccggcatc gtgagcaggg aggcgatcga gcgtgcggtg ctcgacagcg tccccaaggg
     4561 caccgagaac ctgaacatga aagcactgaa acgcggattt gaactcggag agcgagtgtg
     4621 taacttatga aactactgga gtacgaggca aaacagatct tttcggagta cggcataccg
     4681 g
//
LOCUS       DAOSEE010000155         4621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_6551_length_4621_cov_195.348226, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000155 DAOSEE010000000
VERSION     DAOSEE010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6551_length_4621_cov_195.348226"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..425)
                     /locus_tag="PLI76_09780"
     CDS             complement(24..425)
                     /locus_tag="PLI76_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOI62363.1"
                     /translation="MPPVETCYDILGVAPDATPDEIRAAYRRLARQYHPDVNPDPDAS
                     ERFIAIHQAYEVLIDPEARARYDLSLRHQAADAGGQAAWAPGFTWRAQAPGSEAGRPG
                     TIAALVIWSMMVLLMLVLSLLLRLVIGGRQR"
     gene            complement(425..823)
                     /locus_tag="PLI76_09785"
     CDS             complement(425..823)
                     /locus_tag="PLI76_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HOI62364.1"
                     /translation="MQGRGISRGIGSGDLLVSPEPISFLSGVDPETGIVIERGHPLEG
                     QSIAGRVLAFPYGKGSTVGSYVIYALKRNDRAPVAIINTEAEPIIAVGAIIAGIPMVD
                     RLPPEFSCLAPGTRVTVNGDTGEVSYVLDT"
     gene            865..2037
                     /locus_tag="PLI76_09790"
     CDS             865..2037
                     /locus_tag="PLI76_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-activating nucleotidase"
                     /protein_id="HOI62365.1"
                     /translation="MSNMDETVGSSPGTEHDMLNLQIQDLKAQILDYRLKNELLEKEV
                     MQLRKENAQLKRVPLFVAAVVDVLENGEVYLRQQGNNQEYVTTVSEKLHRILKPGMKV
                     AVNNTLSVVRTIGNTYDSRVRVMELDEQPSVTFEQVGGLKEEIEEVREAVEYPLTKPK
                     IYERVGVEPPKGILLYGPPGTGKTLIAKAVARQSRARFIRMSGSELVHKYIGEGAQLV
                     RELFVLARERAPAIVFIDEIDAIGSMRTNDGTSGSAEVQRTLMQLLAEMDGFGNRGNV
                     RIMAATNRIDMLDPALLRPGRFDRIIRIPLPDAGARLEILKIHTAKMSLGKDVDLAGL
                     AELAEQTTGAELQAICREAGMMAVRRDANAVDHEDFLVALRKVKREVAAPDTRMYI"
     gene            2215..2658
                     /locus_tag="PLI76_09795"
     CDS             2215..2658
                     /locus_tag="PLI76_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5804 family protein"
                     /protein_id="HOI62366.1"
                     /translation="MNVLLIQPEGVDLYTTLLKSETSRGALRFYQPDRLEYGIRVRTA
                     SLGSALALASELRWYVQRYVEEVLFEPEKDIFCTSGLARQIYERDVRPGSPWRYRCLY
                     RISGDHLTDTIWMDEGTAPEAYAGRMEPGDTVLEVWCTEEEYPGR"
     gene            complement(2679..3983)
                     /locus_tag="PLI76_09800"
     CDS             complement(2679..3983)
                     /locus_tag="PLI76_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOI62367.1"
                     /translation="MSDKGREEWSSMLGFILASIGSAVGIGNVWRFPYIVGANGGGAF
                     LVPYLISVFLFGLPLMVLEFAIGRSTGSSVASAFQSLRQRFFAVGLAIVVIVCLILSY
                     YLVITSWVLAYALFFALNRPVDFGAFTGSYLPLAFFILSGLAVFATVRSGVREGIERV
                     ARYLIPALVVILLFLVVFALTEPGALEGVRFYLLPDYSRLADPGVWTAAFGQAFFSLS
                     VGMGVMLTYGSYLKRGGLFRSASIIAVADMAIAILAGLMIFPLVFTAGVDPAAGVNLA
                     FVTLPAVFMGMQSGMVLGALFFFMLFAAALTSAVSMLEVPVATLMDSYGYPRRRATLL
                     IFVAVMLLGLPSALSYTALRFEIFGAPFLDLADYTFGTIGLIAAGLVLSVIGGWFMER
                     SRICAEIGGCGWQQRIYMVLIRYCVPAILVITLAATLLQAAL"
     gene            4233..>4621
                     /locus_tag="PLI76_09805"
     CDS             4233..>4621
                     /locus_tag="PLI76_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HOI62368.1"
                     /translation="MSGRYPFLFSIPHGGVSVPPEIRGLVGLSKKEIVFNSDPHTRLL
                     YGFGDRVEALVDFDVSRIFVDTNRPPYDYPPRSQDGVVKVITQDGTPVYRRGQIPGRE
                     LIGTLLKSYYYPFHDRLAGALDASTIE"
BASE COUNT          953 a         1444 c         1465 g          759 t
ORIGIN      
        1 gcgggcaggg atgacagggc gcgttaccgc tgccgaccgc cgataaccaa gcgtaagagg
       61 agcgagagta cgagcatgag gaggaccatc atgctccaga tgaccagggc tgctatggtg
      121 ccgggccgcc cggcctctga tccgggcgcc tgtgctctcc aggtgaaccc cggcgcccat
      181 gccgcctgcc ctcctgcatc ggccgcctgg tgcctgaggg agaggtcgta gcgtgcccgg
      241 gcctccggat cgatcagcac ctcgtatgcc tggtgaatag cgatgaaccg ctcgctcgca
      301 tcggggtcag gattgacgtc cgggtggtat tgcctcgcga gtctccggta ggccgcccgg
      361 atctcatcgg gtgtcgcgtc gggtgcgacc cccaggatat cgtagcaggt ctccacaggg
      421 ggcatcacgt atcgaggacg tatgatacct cgcccgtatc cccgttcacc gtcacccggg
      481 tgccgggggc gaggcaggag aactccggcg ggaggcggtc gaccatcggg atccccgcga
      541 tgatcgcgcc gaccgcgatg atcggttccg cctcggtgtt gatgatcgcg accggggccc
      601 ggtcattccg tttgagcgcg tagatgacgt aagatccgac cgtcgatccc ttcccgtacg
      661 ggaatgcgag cacgcgcccg gcgatggatt ggccctcaag cgggtgtccg cgttcgatga
      721 cgatacccgt ctcggggtcg acgcccgaga ggaacgatat gggttcgggc gaaacaagga
      781 ggtctccgct cccgataccc ctggatatcc ctctgccctg catggtcact gccaccacta
      841 taatgaggtt ggagatccaa ctatatgagt aacatggacg aaaccgttgg cagcagtcct
      901 ggcaccgagc acgacatgct caacctccag atccaggacc tgaaggcgca gatcctggat
      961 tataggttaa aaaacgagtt gcttgagaag gaggtcatgc aactgcgcaa agagaacgct
     1021 cagctgaagc gggtgccgct cttcgttgcc gctgtggtcg acgtgctgga gaacggcgag
     1081 gtatacctcc ggcagcaggg caacaaccag gagtacgtca ccacggtcag cgagaagctc
     1141 caccgcatcc tcaagcccgg gatgaaggtg gcggtgaaca acacgctctc cgtcgtcagg
     1201 acgatcggca acacctacga ctcgagggtc cgggtcatgg aactcgacga gcagccgagc
     1261 gtcacgttcg agcaggtcgg cggcctgaag gaggagatcg aggaggtcag ggaagccgtc
     1321 gagtacccgc tcacgaaacc caagatctac gaacgcgtgg gcgtcgaacc cccgaaaggc
     1381 atcctcctct acggcccgcc cgggaccggg aagacgctga ttgcaaaggc tgttgcccgc
     1441 caatcgcgcg cccggttcat caggatgtcc gggagcgaac tcgtccacaa atacatcgga
     1501 gaaggtgcac aactcgtgcg tgaactcttc gtcctcgccc gggaacgcgc cccggcgatc
     1561 gtcttcatcg acgagatcga cgcgatcggg agcatgcgca ccaacgacgg gacgtccggc
     1621 agcgccgagg tccagcggac gctgatgcag ctcctggcgg agatggatgg ttttgggaac
     1681 cgcggcaacg tccggatcat ggcggcgacg aaccggatcg atatgctgga cccggccctc
     1741 cttcgcccgg gccggttcga ccgcatcata cggattcccc ttcccgatgc cggggcacgc
     1801 ctcgagatcc tgaagatcca taccgcaaag atgtcccttg gaaaggacgt ggacctcgcc
     1861 gggctcgcgg agcttgccga acagaccacc ggtgcggaac tgcaggcgat ctgccgcgag
     1921 gccggcatga tggcggtccg gcgggatgcc aatgccgtcg accacgagga tttcctcgtg
     1981 gcgctcagga aggtgaagcg cgaggtggcg gctcccgaca cccgcatgta tatctgacgg
     2041 ggactcctcc acccccggtt tttccgtgag atactcatcc cggggagacg gcgtcctgtc
     2101 cttcgcgctc attcctgcgc ccggcacccg gatcttcacc tctgcagcct tttgctcttc
     2161 cccatatgca cccctgagga acccttatcg acctcccgcg cccacaccta tgcgatgaac
     2221 gtcctcctca ttcagcccga gggggttgac ctctacacca ccctgctcaa atccgagacc
     2281 agcagggggg cccttcgttt ttatcagccc gacaggctgg agtacggcat acgtgtccgg
     2341 acggcgtcgc tcggaagtgc gcttgccctc gcctccgagc tccggtggta tgtccagcgc
     2401 tacgtcgagg aggttctctt cgagccggag aaagacatct tctgcacatc cgggcttgcc
     2461 cggcagatct acgagcggga cgtgcggccg ggatcgccgt ggcggtaccg ctgcctctac
     2521 cgcatctccg gggaccacct gaccgacacc atatggatgg acgagggcac ggcgccggag
     2581 gcgtatgccg gccggatgga gcccggggat acagtgctcg aggtctggtg cactgaagag
     2641 gagtacccgg gcaggtagag gatcgggtgc gcccccgttc acaacgcggc ctggaggagg
     2701 gtcgcggcca gcgtgatcac gaggatcgcc gggacgcagt agcggatgag caccatgtag
     2761 atccgctgct gccacccgca cccgccgatc tctgcacaga tccgggagcg ttccataaac
     2821 caccccccga tgacgctcag gaccagccct gcggcgatga ggccgatcgt cccgaacgtg
     2881 taatcggcga ggtcgaggaa cggagccccg aagatctcga accggagggc ggtgtagctg
     2941 agggcggacg gcagcccgag gagcatgacg gcaacgaata tgaggagcgt cgcgcgtctc
     3001 ctcgggtagc cgtacgagtc catcagcgtc gccaccggga cctcgagcat ggagaccgcc
     3061 gaggtgagcg ctgcggcaaa gagcatgaag aagaagagcg ccccgagcac catgccggac
     3121 tgcatcccca taaaaacggc aggaagagtg acgaacgcga ggttcacccc ggctgccggg
     3181 tcgactcctg cggtgaagac gaggggaaat atcatgagcc cggcaaggat tgcaatcgcc
     3241 atatctgcga cggcgatgat ggaggcgctc cggaaaaggc ctcctctctt gagataactg
     3301 ccgtaggtga gcataacgcc catgcccacc gagagcgaga agaaggcctg cccgaacgct
     3361 gcagtccaga cgccggggtc ggcaagcctc gagtagtccg ggaggaggta gaaccgaact
     3421 ccctcgagcg ccccgggctc ggtgagcgcg aagaccacga ggaagaggag gatcacgacc
     3481 agggccggta tgagataccg ggcaaccctc tcgatcccct cccgaacccc ggatcgcact
     3541 gttgcgaaga cggcaagccc cgagaggatg aagaaggcga gcgggagata ggagccggtg
     3601 aacgcaccga agtccaccgg ccggttgagg gcaaagaaga gtgcgtaggc cagcacccag
     3661 ctcgttatga ccaggtagta actgaggatc aggcagacga tgacgacgat ggcaaggccc
     3721 acggcgaaga accgctgtcg cagggattga aaggcagatg ccaccgagct cccggttgac
     3781 cggccgatgg caaactccag caccatcagg gggagaccga agagaaacac ggagatcagg
     3841 tacgggacca ggaacgcacc acccccgttg gcgcccacga tgtaggggaa ccgccatacg
     3901 tttcctattc ccacggccga tccgatgctc gcgaggatga agcccagcat cgaagaccat
     3961 tcctcccttc ctttgtcact catcgtttgg aaccccaccc ctgatagtag agggatgttc
     4021 catcttatta tcggtgtggc actccggcgg cgtgagtgga aaatcccccg tgcaagcggg
     4081 ggagtattta taggcatccg accccatcaa gcggcaggat ctgccgggtt atccccgacc
     4141 agtgcacttt tgtatcaggt tttccataca aatacagttg aatacagaat gagcgtcagt
     4201 tcctggagac gccccggcgg ggggaagagg ggttgagcgg tcgctacccg ttcctcttct
     4261 cgatccccca cggcggtgtc tctgttcccc ccgagattcg cggcctcgtc ggcctctcaa
     4321 agaaagagat cgtcttcaac agcgaccccc atacccgtct cctctacggg ttcggcgaca
     4381 gggtggaggc gctggtcgat ttcgacgtct cccggatatt cgtcgatacc aaccggccgc
     4441 cctacgacta cccgccgcgg tcccaggacg gcgtggtcaa ggtcatcacg caggacggga
     4501 caccggtata ccggaggggg cagatccccg gcagggaact gatagggacc ctcctgaaga
     4561 gttactacta cccgttccac gaccgactgg ccggcgccct cgatgcttcc actatcgaga
     4621 t
//
LOCUS       DAOSEE010000156         4579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_6656_length_4579_cov_190.219275, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000156 DAOSEE010000000
VERSION     DAOSEE010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6656_length_4579_cov_190.219275"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PLI76_09810"
     CDS             <1..183
                     /locus_tag="PLI76_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62369.1"
                     /translation="PGWEANRAMMRWREEERLVQWGCTSCMHYEPRSIYCTFIHAPVW
                     VLFTCPMVLDNSRSSR"
     gene            514..1410
                     /locus_tag="PLI76_09815"
     CDS             514..1410
                     /locus_tag="PLI76_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62370.1"
                     /translation="MPGADYIYPNVTEMAQRGGLLERWRLAEPLGCTYVEVPADLVKN
                     RTEVERTGQDIGSILKRSSVELLYEQEAGLPGNLAYLLHTEPAIPRRDGHGRQVKADL
                     RWRDRAWLATFGDMLLDIEEYIGIPPDIIEIHPGSRKNTYADIVAAMYSLIVAHWNAF
                     GVEPLVLLENHADQSVATAGQMEAFWTTLKELHPEIAGLAGIALDPWHLRAAASDEFP
                     GALRRVPPAALKAFHIHNNLVPPTAADRMPWQEVFSVVRSLPARPRIKPVVYQESRVA
                     GAIDFCEGMLAQPRAVLPGQHS"
     gene            1435..2835
                     /locus_tag="PLI76_09820"
     CDS             1435..2835
                     /locus_tag="PLI76_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOI62371.1"
                     /translation="MKPTAAPDLPPGLLWFNTDRPLSLSDLAGRVVLLSFRTFACANC
                     MRLVPEIRRLRKEHPALVVIGVHAPGFEPAAVAGNFAEAVRRSGAGYPVAIDRDHRLR
                     EAFGIRTWPTFVLIDPEGKIVGTTTGEGIAGRLGPTIGRLGGDSAGPLQAGGVSVVKG
                     ALYYPGKVAADHAGMRLFISDTGHNRIVVAGTDGRIIETIGSGEPGNADGSSGKAAFY
                     RPEGVAFDEAAGILYVADTGNHMIRAVSWPDRAVATVAGTGLSVPGALNAPRDITLLG
                     GFLYIAGARRIWRMNLATRETEPYAGSGREALVDGPLMEAAFAGPSGIVTDGEALYVA
                     DREASAIRRIKRGMVETRIGHSLEDFGDLDTIAGMARIHHPAGLAYHQGLIAIADTGN
                     HKIKEFDPETGWVLTRAGSGGQGDRDGLSGDATFNEPGGLVNLGGLWYIADTKNHVVR
                     VYDPAGHVVSTLRLWG"
     gene            3300..3788
                     /locus_tag="PLI76_09825"
     CDS             3300..3788
                     /locus_tag="PLI76_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62372.1"
                     /translation="MSSDPGEALADAVLLRLKFISGFDHGSSALRLDARIAIGIGTVQ
                     YLPKGGSAGEGDGEAFRRSGLELDLMKEAGRGITVKTPWGDVNATLSIFCDVLDRTID
                     GYTKKQAEAVMLTLEGLTQKEIGERVGTSQSAVSYRLKGTGYDLVQKILEWYRLQISS
                     RI"
     gene            3994..4254
                     /locus_tag="PLI76_09830"
     CDS             3994..4254
                     /locus_tag="PLI76_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62373.1"
                     /translation="MIGEDISEDWRNDLKQFASNRKKDPDEPTFKNVIGERALSNWFA
                     AFPTGRPAGKSARPGLPLQFGGNGIGGEPAPADPKEIMLREG"
     gene            4274..>4579
                     /locus_tag="PLI76_09835"
     CDS             4274..>4579
                     /locus_tag="PLI76_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOI62374.1"
                     /translation="MHPTIREKIPEIARIARRRRVKRLGLFGSATGDRFDPAASDIDF
                     VVEFEPMTPAEHAEAYFGLAEDLTRLFCRTIDLVERSAIRNPIFRESVEETCRDIYAA
                     "
BASE COUNT          839 a         1410 c         1555 g          775 t
ORIGIN      
        1 cccgggtggg aggcgaaccg cgccatgatg cgatggcgag aggaggagag gctggtgcag
       61 tgggggtgca cctcgtgcat gcattacgaa ccgcggtcta tctactgcac gttcatccac
      121 gcacccgtat gggtcctctt cacctgcccc atggtcctcg ataacagcag gtcgtcccgg
      181 tagtcaggag atcttttttt caggatcatg agtcgaggaa ccccttcttc tcgccagggg
      241 aggaatcgat cccctgcgta tcctgataca cgtttgcccc gcacgcaccc gtgacgccgg
      301 tggaggaggt gctcgcggcc gcagccacgg ggggaacggc acgatctccc ggggggtgca
      361 gaacgcgagc acccgggagg gcgctcttcg tccgcgggcc gggtgcatgg gggcaccgag
      421 aagaataggt cgggttgacg gcctgccggg gaggcggtac cgcccgcccc caacatattt
      481 gtagcccttc cgccatgggg tagcccgatc acgatgccgg gcgcagacta catctatcca
      541 aacgtcaccg agatggcgca gcggggaggg ctgctcgaac gctggcgcct cgccgaaccg
      601 ctcgggtgca cgtacgtcga ggtccctgcc gaccttgtca agaacaggac cgaggtggag
      661 cggaccgggc aggatatcgg ctccatactc aagaggtcgt ccgtcgagct cctttacgag
      721 caggaggcgg gcctcccggg caacctcgcg tatctcctgc acaccgagcc cgccatcccc
      781 cggagggacg ggcacgggcg gcaggtgaag gcggacctgc ggtggcgcga ccgcgcctgg
      841 ctggcaacgt tcggggatat gctcctcgat atcgaggaat atatcggtat tcccccggac
      901 atcatcgaga tccatcccgg cagccggaag aacacctacg ccgatatcgt cgcggcgatg
      961 tactccctga tcgtcgccca ctggaacgcc ttcggcgtgg agcccctggt gctgcttgag
     1021 aaccacgcgg accagagcgt ggcgacagcg ggccagatgg aggcgttctg gacgacgctc
     1081 aaggaacttc acccggagat cgccggtctt gccgggattg cgctcgatcc ctggcacctc
     1141 cgcgccgccg cgagcgatga gtttcccggg gcgcttcgcc gggtcccgcc cgcggccctc
     1201 aaggccttcc acatccacaa caatctcgta ccgccgacgg cggccgaccg gatgccctgg
     1261 caggaggtct tctcggtggt ccggagtctg ccggcacggc cccgtatcaa acccgttgtc
     1321 taccaggaga gccgggtcgc cggagcgatc gacttctgcg aaggtatgct cgcacaaccg
     1381 cgggcggtgc tgccgggcca acattcataa attcccggtg ccagagggtg ccgcatgaaa
     1441 ccgacggctg cgcccgatct ccccccgggc cttctctggt tcaacaccga ccgccccctc
     1501 tccctctccg acctcgcggg cagggtcgtg ctgctctcgt ttcggacgtt tgcctgcgca
     1561 aactgcatgc gccttgtgcc ggagatccgg cgtctccgaa aggagcaccc cgctctcgtg
     1621 gtcatcgggg tccacgcgcc cgggtttgag cccgccgcgg tcgccgggaa cttcgccgag
     1681 gcggtccggc gctccggggc gggatatccg gtcgcgatcg accgcgatca ccggctccgg
     1741 gaggccttcg gcatcaggac ctggccgacg ttcgtcctca tcgatccgga ggggaagatc
     1801 gtcgggacga cgaccggcga ggggatagcc gggcggctcg gcccgacgat cggccggctt
     1861 ggcggtgact ccgccgggcc gctgcaggcg gggggcgtat ccgttgtaaa gggcgccctc
     1921 tactaccccg ggaaggtagc ggcggaccac gcgggtatgc ggttgttcat cagcgatacg
     1981 ggccacaacc ggatcgtcgt cgccggcacc gacgggcgga tcatcgagac gatagggtcc
     2041 ggggagccgg ggaacgccga cggatcgtct ggcaaggccg cgttctaccg gccggagggt
     2101 gtcgccttcg acgaggcggc cggcatcctc tacgtcgcgg acaccgggaa ccacatgatc
     2161 cgggcggtat cctggcccga ccgggcggtc gcgacggtcg cggggacggg gctctccgtc
     2221 cccggggcgc tgaacgcgcc gcgggacatt acgctgcttg gggggttcct ctacatcgcg
     2281 ggggcacgcc ggatctggcg gatgaacctt gccacccgcg agacggagcc gtacgccggg
     2341 tcgggccggg aggccctggt ggacgggccg ctcatggagg ccgcgtttgc cggcccgtcg
     2401 gggatcgtca ccgacggcga ggcgctctac gtggccgaca gggaggcgtc ggcgatccgc
     2461 cggatcaagc gggggatggt cgagacgcgg atcgggcatt cacttgagga cttcggcgac
     2521 ctcgacacca tcgccggtat ggcgcgtatc caccaccccg cgggcctggc ctaccaccag
     2581 gggctgatcg ccatcgccga taccggcaac cacaagatca aggagttcga cccggagacg
     2641 ggctgggtcc tcacccgggc cgggagcggg gggcagggcg accgcgacgg gctctcgggg
     2701 gacgcgacgt tcaacgagcc cggcggcctg gtcaacctcg gagggctctg gtacatagcc
     2761 gataccaaaa atcacgtcgt cagggtctac gatccggccg gccacgtggt ctcgaccctg
     2821 aggctctggg ggtagggcgg gtttgctggc gagctgcagc cgccagggga agaccgcccg
     2881 cacccccctc ccgggccgcg gcccgtgtca cataagggaa ccggccatac cggatcgata
     2941 tagatgggcg ccgccctgtg cggatagggt ctcgcagctc tcaccccggc cgagatgact
     3001 ggcatcatcc gtaactggcg gttctgggta atatataagt aatataactc atgatcttat
     3061 gatataagaa aagatactca tatacggcgg ggacaggaaa cccctgagag gaaggcgacg
     3121 atgccgccat gacggaagga agggtttacg cggtcctcac gggagatctg gtccgatcga
     3181 gggctatcaa agatgcgtat ggtgaggagt gtatacaccg cctgaaggat acgctggctg
     3241 agatcgggag aaactacaca gtcccgttct cgatataccg tggggacagt tttcaggggg
     3301 tgtcttcaga tcccggggag gcgctcgcgg atgcagtcct cctgcgcctc aagttcatca
     3361 gcgggttcga tcacggcagc agcgccctcc ggctggatgc acgaattgca atcgggatcg
     3421 gaaccgttca gtatctgccg aagggtggtt ctgcgggaga gggggatggc gaggcgttcc
     3481 ggcgttcggg tctggagctg gatcttatga aggaagccgg gcgcggtatc accgtaaaga
     3541 ccccctgggg ggatgttaat gcgacgctat cgatcttctg cgatgttctg gaccggacca
     3601 ttgacggata tacgaagaag caggccgaag cggttatgct gacactggaa gggctcacgc
     3661 aaaaggagat cggggaacgg gtcggcacaa gtcagtctgc ggtgtcatat cgtctgaagg
     3721 ggacgggtta cgacctcgtg cagaagatcc tcgaatggta ccggttgcag atcagctccc
     3781 ggatctagcg atcgggagat ggcgaagtgc catgacccga gcccggcctc cagcggagga
     3841 ccggtttttt gggccgggtg acacgattgc cgacccgtgg atgccatgac tgttttgctt
     3901 cgccgggatt ctgtgaaccg cgtgatcaat gacatacctc aatggactgc cgggatgaca
     3961 gggggtgctc gcccccggca cctcgaatga gatatgattg gcgaagatat tagcgaagat
     4021 tggcggaatg atctcaagca atttgcctca aatcgtaaaa aagatcctga tgaaccgaca
     4081 tttaagaacg tgattgggga aagggctctc tcgaactggt tcgcggcctt cccgacagga
     4141 cgccccgcgg gtaagagcgc caggcccggg ctcccgctgc agttcggcgg caacggcatc
     4201 gggggggagc cggcacccgc cgatccgaaa gagataatgc tccgggaggg gtaaatcctc
     4261 atagagaagt ccgatgcacc ccaccatccg cgagaagatc cctgagattg cccgcatcgc
     4321 ccggcgccgg cgcgtgaaga ggctcgggct cttcggttcg gcgacaggcg accggttcga
     4381 ccctgccgcg agcgacatcg acttcgtcgt cgagttcgag ccgatgactc cggcggagca
     4441 cgcggaggca tacttcggcc tcgcggaaga cctgacccgg ctcttctgcc gaacgatcga
     4501 tctcgtcgag cgatcggcga tcagaaaccc aattttccgc gagtccgtgg aagagacctg
     4561 cagggatatc tatgctgcc
//
LOCUS       DAOSEE010000157         4530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_6734_length_4530_cov_242.394860, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000157 DAOSEE010000000
VERSION     DAOSEE010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6734_length_4530_cov_242.394860"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PLI76_09840"
     CDS             complement(<1..345)
                     /locus_tag="PLI76_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOI62375.1"
                     /translation="MGRSDMQGAVQETAGSDRAPESIILRVALLSLAVNIGLVVAKLA
                     LSVYSGSLALRADGIHSFVDVVASLALIAGVLLSTRRRRDFPYGLYKVENVVAVIIAL
                     LIFFTAFEIAAEA"
     gene            complement(470..1078)
                     /locus_tag="PLI76_09845"
     CDS             complement(470..1078)
                     /locus_tag="PLI76_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOI62376.1"
                     /translation="MIDLSIFLAEHPYIDHSSPRIRAKAGELFSGIESSLERTRIAYE
                     FVRDEIPHSFDIGSDAITARASDVLARGTGICHAKANLLAALLRGFGIPAGFCYQHIT
                     LADDDSLGYCIHCYNAVHVEGRWIFLDARGNAGGRQALFSPGKPILAYPNRSEYDEYF
                     WKGIYASPQMGVMRMLDAAVTRQDVIDNLQDYIEGEPDIPGW"
     assembly_gap    1163..1262
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1370..1960
                     /locus_tag="PLI76_09850"
     CDS             1370..1960
                     /locus_tag="PLI76_09850"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOI62377.1"
                     /translation="MDIFDEIYRGTPPWDIGRPQKAFIELAGRGEIRGSVLDIGCGTG
                     EHALFVAVAGHEALGIDTAPLAIQKAREKAAERGLPARFLVGNVLNLSGLNGRFDTVI
                     DSGLFHTLSDKDRPVLAESLATVLAPGGRYFMLCFSDRNPGEYPLPRRIAMAEIRDTF
                     QGGWSIDYVRPAVFENTVQADGHLAWLASISRKAQE"
     gene            complement(2167..3474)
                     /locus_tag="PLI76_09855"
     CDS             complement(2167..3474)
                     /locus_tag="PLI76_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOI62378.1"
                     /translation="MTACAQEYETKRTNPADAIRLVKSGNTVVYGMSISQPPALLTAL
                     ADAARAGSLKDVRIYTFLPREHAARSVLAPDLCDCLENYSWFVGAVDRDLTKVGLNYH
                     VPSYLHQIPTLVAEFMEADVVMTTVSPMDTAGYFSFGTGNDYISTAARHGKRFVVEVN
                     RNMPRVFGDSQVHISEVDAIVENHVPLLEIAAPDPKPEDDIIGPAIAEMIPDGATIQL
                     GIGNLPNAVARYLTGHTDIGVHTELFTEGMVDLIEQGVITGRRKTLHPRKHVFTTAAG
                     TRRMYEFMDDNPAMESYPASYVCDPGIIAKNDTMISINSVLEVDLLGQANAEYLGGTT
                     FSGTGGQLDFVRGAFASRGGKSILAFYSTAKGGTISRIVPRLPLGSIVTTPRADTDFL
                     ATEHGIVSLKGRSTKERALSIISLAHPKFRDWLMEEAENMYLL"
     gene            complement(3907..4386)
                     /locus_tag="PLI76_09860"
     CDS             complement(3907..4386)
                     /locus_tag="PLI76_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62379.1"
                     /translation="MKRYSRQDQTTMAVWAAYCAERVLPLFESVYPEDGRPHRAIEAC
                     RAWVETGVFRMVDIRGASLAAHAAARDAEEGSAARFAARAAGQAVATAHVPQHAYGAA
                     YYALKAVAAANLADVAREREWQAERLPEGLREEIMERIIILERSGGVSIKLRKGEGF"
BASE COUNT          820 a         1393 c         1361 g          856 t
ORIGIN      
        1 cgcctccgcc gcgatctcga aggcggtgaa gaagatgagg agggcaatga tcaccgcgac
       61 gacgttctca actttgtaga gcccgtaggg gaaatcccgg cgcctgcgtg tggagagaag
      121 cacgcctgcg atgagcgcaa gcgaggcgac gacgtccacg aacgagtgga tgccgtccgc
      181 acggagggcc aggcttccgg aatagacgga gagtgccagt ttcgcgacga ccagcccgat
      241 gttgacggcg agggagagga gggcgacacg caggatgatg gattccggcg cccgatccga
      301 tcccgctgtc tcctgcaccg ctccctgcat atcggatcgc cccataatgc attccttggc
      361 gtggcgcatc catcaatcta accattgtac ccaatccgcc ctgacaggcg ccagagcagc
      421 ggctccgccg gcgcgaacgg ccgtgttacc cattccgccg catctcgtat caccaaccgg
      481 ggatatcggg ctcgccctcg atatagtcct gcaggttatc gatgacgtcc tgccgggtca
      541 cggcggcgtc gagcatccgc atgacgccca tctgcggcga tgcatagatc cccttccaga
      601 agtattcgtc gtactcactc cggttgggat acgcgaggat gggttttccc ggcgagaaga
      661 gcgcctgccg gccgccggcg ttcccgcgtg cgtcaagaaa gatccagcgg ccttccacgt
      721 ggactgcgtt gtagcagtgg atgcagtagc cgagcgagtc gtcgtccgca agggtgatat
      781 gctggtagca gaacccggcg gggattccga atccgcggag cagggccgcg agcaggtttg
      841 ccttcgcgtg gcagatccct gttccccggg caagcacgtc ggaggccctc gccgttatgg
      901 cgtccgagcc gatatcgaac gagtgcggga tctcgtcgcg aacgaactcg tatgcgatcc
      961 gcgtccgttc gagagagctc tctatcccgg agaacaactc tccggccttc gcccggatac
     1021 gcggcgacga gtgatcgata taggggtgct ctgcaaggaa gatgctcagg tcgatcatgc
     1081 gaggagggtg ttgccgggtg gttgtactgc gttggcgccg gggttattta agtatccggg
     1141 cggttgtgct gtccggcatg ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnatgctgca gtgctcccct cacctcagac cgtatgaccc ccgcctctca gtccaacctg
     1321 ccttgagcag cacaaaaccg gttaagccgc acacggccca tactgcggta tggacatctt
     1381 cgacgagatc taccggggca cgccgccctg ggacatcggc cggccccaga aggcgttcat
     1441 cgaactggcc gggagggggg agatcagggg ttccgtgctg gatatagggt gcgggacggg
     1501 agagcacgcc ctctttgttg cggtcgccgg gcacgaggcg ctcggaatcg acaccgcccc
     1561 ccttgcgatc cagaaggccc gggagaaggc cgctgaaaga ggcctcccgg cgcgcttcct
     1621 cgtcggtaac gtcctgaacc tctccggcct caacgggaga ttcgataccg tcatcgattc
     1681 ggggctcttc catactctgt ccgacaaaga ccggccggtc ctcgcggaga gcctcgcaac
     1741 cgttctcgcg cccggcggga gatacttcat gctctgcttc agcgaccgaa accccggcga
     1801 ataccccctg ccgaggagga ttgcgatggc ggagatccgg gataccttcc agggtgggtg
     1861 gtccatcgac tatgtcaggc cggcggtctt cgagaacacc gtccaggccg acgggcacct
     1921 ggcctggctt gcgtcgatat cgagaaaggc gcaggaatga cgtgatcggc cgtgacggca
     1981 cgacgcagac gctgatcgag cggggcggag ggtgctctac cgattttctc tcatcgctcc
     2041 cggtgagatg gaactcgtct acgccgaggg aagcgatctc ttcaacatcg cagtcggctc
     2101 gctgaaaaat atctgagagt cttttcctct ttcgtttgaa gcaaccgccc gggcgggaga
     2161 ccccgttcag agcagataca tattctccgc ttcctccatc aaccagtccc ggaactttgg
     2221 gtgggcgaga gagatgatcg agagcgcccg ttccttggta gacctgcctt tcaggctgac
     2281 gatcccgtgt tcggtagcaa ggaaatcggt atcggcccgc ggggtggtga caatggagcc
     2341 gagcgggagg cggggaacga tgcgggagat ggttccgcct ttcgccgtgg agtagaacgc
     2401 gaggatggac ttgcctcccc gcgaggcgaa cgccccccgc acgaaatcca gctggccgcc
     2461 ggtgccggag aacgtcgtgc cgccgaggta ctccgcattc gcttgcccca gcaggtccac
     2521 ctcgagcacc gagttgatcg agatcatggt atcgtttttt gcaatgattc cgggatcgca
     2581 gacgtaggag gcggggtaac tctccatcgc cggattgtca tccatgaact cgtacatgcg
     2641 ccgtgttccg gccgccgtgg tgaagacgtg cttcctcgga tggagcgtct ttctcctccc
     2701 ggtgatcacc ccctgctcga tgaggtcgac catgccctcg gtgaagagct cggtatggac
     2761 gccgatgtcc gtatgcccgg tcaggtatcg ggcaaccgca ttcggcaggt tcccgatccc
     2821 gagctggatc gtcgcgccgt ccgggatcat ctcagcaatc gccggcccga tgatatcgtc
     2881 ctccggtttt gggtcgggag cggcgatctc gaggagcggg acgtggttct cgacgatcgc
     2941 atcgacctcc gagatatgca cctgtgaatc gccgaagacc cggggcatat tccggttcac
     3001 ctcgacgacg aacctcttac cgtggcgggc tgcagtcgag atatagtcat ttcccgtccc
     3061 gaaactgaag tagccggccg tatccatcgg cgagacggtc gtcatcacga cgtcggcctc
     3121 catgaattcg gcaacgagcg tggggatctg gtgaaggtag gaggggacgt ggtagttcag
     3181 cccgaccttc gtcaggtccc ggtccacggc acctacgaac cacgagtagt tctcgaggca
     3241 gtcgcagaga tccggtgcaa ggaccgaacg ggcggcatgt tcccggggga gaaacgtgta
     3301 gatcctgaca tccttcagcg accctgcccg ggcggcatcg gcaagagccg tgagcaacgc
     3361 cggcggttgg gaaatgctca tcccgtagac cacggtattt ccactcttca ccaaccggat
     3421 tgcatccgcg gggttggttc tctttgtctc gtactcctga gcacaggcgg tcatgtagta
     3481 cctcgtaacc ggtacgtccg gtcataacgt actatggagg cgcccctgta ttaatcctgg
     3541 ccggcaaatc cattttctgc caaaatatcg cctgtatcgg taaaatgacg cagtacatcg
     3601 cgttttctga gagcccgccc cccctcgccc ggtcttgaaa ctcacgaccc cgtagcaccg
     3661 atcaagcggg aggggcggga tcgcctgtgt gtacgtccgc gccgtgccga agaccggggt
     3721 catgccgtgc cgcccgcgac cgcgacttcg gcccttgtca tcgggcgaat cgtgagctca
     3781 cccgatgctg ccgtggttat gggtgtacac caaaggggga tgatggttcg gaaccgaccc
     3841 cgctcccctc acgccgcggc gccccggatg cgccgaacgg cgcaggccca ctgccccgcg
     3901 atcagatcaa aacccctcgc cttttcgtag tttgatagaa accccgccgc tacgttctaa
     3961 aatgataatc ctctccatga tctcctcccg gagcccctcc gggagccgtt ccgcctgcca
     4021 ctcccgctcc cgggcgacat ccgccaggtt cgccgccgca acggccttga gggcatagta
     4081 tgccgccccg taggcgtgct ggggaacgtg ggcggtcgcc accgcctggc ccgcggcccg
     4141 tgccgcgaat ctggccgcgc tcccctcctc cgcatcgcgg gcggcagcat gagcggcgag
     4201 cgaggcaccg cggatatcga ccattctgaa cacgcctgtt tcgacccacg cccggcacgc
     4261 ttcgatggct ctgtgcggcc ggccgtcctc cgggtagacg ctctcgaaga gcgggaggac
     4321 tcgttcggcg caatacgcag cccagacggc catcgttgtc tgatcctgtc tactgtatct
     4381 cttcatggta caccaccccg ggaaaacccc tcttcccgga gagaagggga actatatccg
     4441 cgtgccttct tcgataatcc tgacgtaatc cacaaacatg tactgtttat atcgctcttt
     4501 tccggtgatc tcttcaagga ttccgatatc
//
LOCUS       DAOSEE010000158         4495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_6813_length_4495_cov_257.129054, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000158 DAOSEE010000000
VERSION     DAOSEE010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6813_length_4495_cov_257.129054"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="PLI76_09865"
     CDS             <1..545
                     /locus_tag="PLI76_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (guanine(10)-N(2))-dimethyltransferase"
                     /protein_id="HOI62380.1"
                     /translation="VIKYDRGVEPLFCYAHEHFHRLHLRLREGAAAADRSLARIGYVM
                     QCPRCLYRTEQTGMLPEPEGCALCGADLVPVGPLWTGRINDDGTLAAMQEALARITVG
                     TGPRIARLLAICRQELDTSSHYDYHVIAKSLRVSPGGIGTVVDRLAALGYRASRAHYS
                     GTAIKTDAPLPVLESVISGG"
     gene            complement(532..1647)
                     /locus_tag="PLI76_09870"
     CDS             complement(532..1647)
                     /locus_tag="PLI76_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOI62381.1"
                     /translation="MSSVEEQIRELEDEIKNTPYNKATSKHIGRLKAKLAKIRDEAVA
                     RAMASSGGGEGYSVKKSGDATVVLVGFPSVGKSTLLNRLTGFDLSETADYAFTTVSVI
                     PGAMEHRGAKIQVLDIPGLIAGAAMGKGRGKEVIAVVRSADLILVLVDVFNEQHTDVL
                     LRELYDAGIRINRQRPDITIRKTGNGGVRLNTVGAVDLDLEEVRSILAENKIVNADVL
                     VRDGATQDDFIDAMIGNRVYVPAFIAVNKVDLVDGKTRAEIEEELTERFGEPPIMVSA
                     HSGYRIEDLKDAIFDDLGFMRVYLKPVGGPADMDEPLIIRSPATVEDVCNRLHREFAD
                     KFRYAKVWGRSAKHDAQRVGITHQLADGDVLSIVTRR"
     gene            1836..1910
                     /locus_tag="PLI76_09875"
     tRNA            1836..1910
                     /locus_tag="PLI76_09875"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1872..1874,aa:Glu,seq:ctc)
     gene            complement(2066..2476)
                     /locus_tag="PLI76_09880"
     CDS             complement(2066..2476)
                     /locus_tag="PLI76_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62382.1"
                     /translation="MLTRSEYEAHFRDCEENLLVQQSNGIYTVTPRDGAACPHWDRGG
                     CAVYSSRPIDCRLYPYRMARIDGRDRKCTITLKMSTLCPKVDTLSRSMPETEARALVT
                     KFWREAAGDETGIVVRCESGLVDRLRSRLMAVFR"
     gene            complement(2728..3018)
                     /locus_tag="PLI76_09885"
     CDS             complement(2728..3018)
                     /locus_tag="PLI76_09885"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOI62383.1"
                     /translation="MRDNKPPGKAAAPDETALQVRLYALGLQACGRKVDPASLAFLQD
                     SSVVGADVPGPDTARATARSYIQGINGGEFPAKPGPFCSRCDYGAICCWRAG"
     gene            complement(3137..>4495)
                     /locus_tag="PLI76_09890"
     CDS             complement(3137..>4495)
                     /locus_tag="PLI76_09890"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOI62384.1"
                     /translation="LGLRTVEASLIRDGFSEEEVAVVHPLDFKSMIGDETRIVAIGVH
                     DPLGINPPTSTFVDIARTGPPYNRIKFLELVKNPLLSGLTTIVGGKGAWQVSDPAVME
                     KLHIDHIHQGMGEVSMPRVCRQILDGEEVPRIIEGEEVPAEDIPNILRPTIHGLVEIS
                     RGCGRGCAFCTPGGLKVVHKSLDHIARDIEVNVRAGSTTALLHSEDVLRYGSKGIAAE
                     PEKVLRLVDRVTAIEGVETLACSHIALATAYHHPDLVDQVSEKALSLPKAIWIGSQTG
                     IETGSPRLMEQHMGGKCLPSKPGTWHDIVVESISHLSDAGWVLACTMVVGLPGETEED
                     VLLTRDLVEELRGLRVFLVPMNFVAMGDSRLSLEDSFTADKMTSAHWALLGSCIEHDV
                     RLARGIKDAVYTGSLPVRLLGKYVSERLIRGGESFARSLKDGNPPRDYSVAGESYLVP
                     EI"
BASE COUNT          793 a         1446 c         1378 g          878 t
ORIGIN      
        1 aggtgatcaa gtacgaccgc ggggtggaac cgctcttctg ctacgcgcac gagcatttcc
       61 atcgcctgca cctcaggctc cgggaagggg cggcggcggc cgaccggtcg cttgcacgga
      121 tcggctacgt gatgcagtgc ccccgctgcc tctaccggac ggaacagacc ggtatgctcc
      181 ccgaaccgga ggggtgcgcg ctctgcggtg cggacctcgt gccggtcggc cccctctgga
      241 cggggaggat caacgacgat gggacgcttg ccgcgatgca ggaggcactt gcccgcatca
      301 cggtcgggac cgggccgcgg atcgcgcggc tgctcgctat ctgccggcag gaactggata
      361 cgtcgagcca ttacgactat cacgtcatag caaaatcgct ccgggtctcg cccggcggca
      421 tcgggacggt cgtcgaccgc ctcgcggccc tcgggtaccg ggcgagccgc gcgcactact
      481 cgggaacggc cataaagacc gatgctccgc ttcccgtcct ggagagtgtg atcagcggcg
      541 ggtgacgatg gagaggacgt ctccatccgc gagctggtgg gtgatcccga cgcgctgcgc
      601 gtcgtgcttt gccgaccggc cccagacttt tgcgtaccgg aacttgtccg cgaactcccg
      661 gtggaggcgg ttgcagacat cctcgaccgt cgccgggctc cggatgatca gcggttcgtc
      721 catatccgcc gggccgccga ccggcttcag gtagacccgc atgaatccca ggtcatcgaa
      781 tatggcgtct tttaagtcct cgatgcggta gccgctgtgt gccgatacca tgatgggggg
      841 ttcgccgaac cgttcggtca gttcttcctc gatctccgcc cgcgtcttcc cgtccaccag
      901 gtcgaccttg ttgaccgcga tgaacgcggg gacgtagacc cggttgccga tcatcgcgtc
      961 gatgaagtcg tcctgcgtgg caccgtcgcg gaccaggacg tcggcgttga cgatcttgtt
     1021 ctccgcgagg atcgagcgga cctcttcgag atcgagatcg acggcgccga cggtgttcaa
     1081 ccggacaccg ccgttccccg tctttcttat ggtgatatcg ggtctctgcc ggttgatccg
     1141 gatgccggca tcgtagagct cgcggagaag gacgtccgtg tgctgctcgt tgaagacgtc
     1201 gaccaggacg aggatcaggt cggcgctccg caccacggcg atgacctctt tcccgcgccc
     1261 tttgcccatg gcagcgccgg cgatgaggcc gggaatatcc aggacctgga tctttgcgcc
     1321 ccggtgctcc atcgcgccgg ggatgacgga gacggtcgtg aaggcgtaat ccgccgtctc
     1381 gctgagatcg aaaccggtga gcctgttgag cagcgtactc tttccgacgg acgggaaacc
     1441 gaccaggaca acggtggcgt ctcccgactt cttcacggag taaccctcgc cgcccccgga
     1501 ggaggccatg gcccgggcta ccgcctcatc gcgaatcttc gcgagtttcg ccttgaggcg
     1561 accgatgtgc ttggaggtcg ccttgttgta cggggtattc ttgatctcgt cttcaagttc
     1621 ccgtatctgc tcttcgacgc tgctcattac tgccggtata ggtacgccgg gcagctactt
     1681 attgatgtct gtgatggaga acgtggattg atgggggaat cccggcccgg atagggctcc
     1741 gattacaggg ttgccggcag gggtgaaagg aggggggtac gcgacatttt taaatagtag
     1801 cgtatcagat aatgtagagc acattggtgc aatgcgctgc tatagtgtag accggccaat
     1861 catgagggac tctcactccc tcgactgggg ttcgaatccc catagcagca ttctgttttt
     1921 cgggttcaaa cctgacagcg agcggtttta tgcctcccct gcacgtgaac gttcaacagt
     1981 accaacggcc ggagggatag aggagggagt acaaggcgga tgcctgccgg taatgggcac
     2041 ggcagggaga gatctcctga ccccttcacc ggaacaccgc cataagccgg gaccgcagcc
     2101 gatcgacgag ccctgattcg caccggacga cgatgcccgt ctcgtctccg gcagcctctc
     2161 tccagaactt cgtgacaagt gcgcgtgctt cggtctccgg catcgacctg ctcagcgtat
     2221 caaccttcgg gcagagcgtg ctcatcttga gggtaattgt acatttcctg tctctcccat
     2281 cgatccgcgc cattctgtag ggatagagcc ggcagtcgat cggtcggctc gaatagacgg
     2341 cgcaaccgcc acgatcccag tgcggacagg ctgcaccgtc tcttggcgtt acggtgtaga
     2401 tgccgttgga ctgttgtacg agaaggtttt cctcgcagtc tctaaagtgc gcctcgtact
     2461 cgctcctcgt caacataaga ccggtcaatc gcgtacaaca gtgttgatcc gtgctgcatg
     2521 tgccgcacag attatccatc acgcttccca ttccctgacc tcctgacacc tgcccggtgt
     2581 cggggatccc ttatgtatgt atggtgctgc taacgtgggg cgtccttgag accctgctcc
     2641 tcgaatgaaa gatccttcgt ctgggcacgc ggaggtccat gacgcccctg aagaagcccg
     2701 gtggagcggg cggcgtacct cccgccccta ccccgccctc cagcagcata tcgccccgta
     2761 gtcgcaccgc gagcagaatg gccccggctt tgccgggaac tccccgccgt tgatcccctg
     2821 gatgtaactc cgggccgtcg ctctcgcggt atcaggcccg ggcacatcgg cgccgaccac
     2881 cgatgagtcc tgcaggaacg caagcgacgc cgggtccacc ttcctgccgc aggcctggag
     2941 gccgagcgcg tagagccgga cctgcagtgc ggtttcgtcc ggcgcggcgg ccttaccggg
     3001 tggcttgttg tcccgcacct tgagcccgtc gcgtagccgc ccatgcggcg catatcccgg
     3061 agcccggcgt ctcctcgcac ccgacacagg taaaacagcg gcaggaccct cccgtcctcc
     3121 cggagagccg gaaaaattag atctcgggaa ccaggtaact ctccccggca accgaatagt
     3181 cgcggggcgg gttgccgtcc ttcaggctcc gggcaaacga ttcgcccccc cggatgagcc
     3241 tctccgagac gtacttgccg aggagccgca ccggcaagct gccggtgtag acggcatcct
     3301 tgatgccccg ggccagacgc acatcgtgct cgatgcagga accgaggagt gcccagtgtg
     3361 cagaggtcat cttatccgcc gtaaaagaat cctccagcga gaggcgggag tcccccatgg
     3421 caacgaagtt catggggacc aggaatacac ggagacctct gagttcctcc accaggtccc
     3481 gggtcagcag gacatcctct tccgtctctc ccggcagacc gaccaccatg gtgcaggcga
     3541 gcacccagcc ggcgtcgctg aggtgagaga tgctctccac gacgatatcg tgccacgtcc
     3601 cgggttttga cggcaagcat tttcccccca tatgctgctc catcaggcgg gggctccccg
     3661 tctcgatgcc ggtctgagag ccgatccaga tcgccttcgg caacgaaagg gccttctccg
     3721 agacctgatc caccaggtcg gggtggtgat aggcggtggc aagcgcgatg tggctgcagg
     3781 caagcgtctc cactccctcg atagccgtca cccggtccac gagccggagc actttttccg
     3841 gttccgccgc gatccccttc gaaccgtatc gcagcacgtc ctcggagtga aggagcgctg
     3901 tggtgcttcc ggcccgcacg ttcacctcga tgtcgcgggc gatatgatcg agggatttat
     3961 gcaccacctt cagacctccg ggtgtgcaga aggcgcagcc cctcccgcat ccccgcgaga
     4021 tctccaccag gccgtggatg gtcggccgca ggatgttcgg tatatcctcg gccggaacct
     4081 cttctccctc gatgatgcgg ggaacctcct cgccatccag gatctgccgg cagacgcgag
     4141 gcatcgagac ctcccccatc ccctggtgga tgtggtcgat atgcagtttc tccatgaccg
     4201 cagggtccga gacctgccag gcgcctttgc ccccgacgat ggtcgtcagg ccggagagga
     4261 ggggattttt cacgagctcc aggaacttga tgcggttgta tggcggacct gtccgggcga
     4321 tatccacgaa tgtcgatgtc ggcgggttga tccccagggg atcgtgcacc ccgatcgcca
     4381 cgatccgtgt ctcatcgccg atcatactct tgaagtccag gggatggacc acggcaacct
     4441 cttcttctga aaaaccgtcg cgaatgagcg acgcttcgac agtccgcagc cccag
//
LOCUS       DAOSEE010000159         4492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_6823_length_4492_cov_255.240929, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000159 DAOSEE010000000
VERSION     DAOSEE010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6823_length_4492_cov_255.240929"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="PLI76_09895"
     CDS             <1..662
                     /locus_tag="PLI76_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOI62385.1"
                     /translation="TFDPSVLGVFVFGLVAGICPCNSVLCLGLIGYLTSGKTDLTLAG
                     ILRLTIAFSFGTILVLLPLGVIAGYLGEYLLFLDETIAWAIGGVLMILMGLQLLHAYK
                     PPIRRIFNFFRAPISYSVTGAFLLGLSFGAITVGRGAPMLLIVLTYIALYQTAIEGLF
                     TIFVYAVGLSIPLIVLSSVGGAVGKKVKEKARMSGEVFDRIIGIAIVLIGVYFLTLAF
                     F"
     gene            complement(667..1227)
                     /locus_tag="PLI76_09900"
     CDS             complement(667..1227)
                     /locus_tag="PLI76_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62386.1"
                     /translation="MSGNIIHPLPRSRFSPALPPLVAAVTAVIWAGTARIVSGTPGPA
                     PPFVLPVFILVIVALVVAGAVTPLPLIEGAVPVKRQRQAMLVAIAASVPFVGALVINP
                     ERWPGGPAPLIAESVPIFGWFFDGIMSVLPLAADTPTYKLFFSAFAVFGFYLECVLVA
                     AILYVVLRVLAALDPGPRGESRPEEE"
     gene            complement(1402..2421)
                     /locus_tag="PLI76_09905"
     CDS             complement(1402..2421)
                     /locus_tag="PLI76_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOI62387.1"
                     /translation="MATESMVSGKVQMDRVSRGMKTERVFIVARKEFTDQVTGWRFLV
                     ILALFLAIALVGTYSGVVSYERELDRYSEQLAAMDDRFDGPERMMPTKPPVADIYSSM
                     FLTLISCGGLLAIAVGFDLVSREKESRSLKSILSHPVYRDEIINGKALGGIALLALVV
                     GGVLAISTALLLVFSIVPAPGDLWMVLTYAGVTLLFFVTFFSIALAISTLCRESGSAL
                     LLSMIVFIFLVLLAPYATTHIGMAFMMEEPDPAAYGGDISSEEFQAAITAYYGQFNLI
                     QSITNLVSPQMATNALIQGISNSSGATLEDMLGRIWSSIAALTIYPVIFFAIGYTKFM
                     RMDIR"
     gene            complement(2786..3562)
                     /locus_tag="PLI76_09910"
     CDS             complement(2786..3562)
                     /locus_tag="PLI76_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62388.1"
                     /translation="MTKATLRIIIALAIVCCTGAMIVTVHLPDSYPHPEVGNVTPEGS
                     PLGSDLPGGISSNATPNEVPEEMQEVRNMTPERSSQGLYVHPFVRQALEEANMTAEVL
                     PEELTEEIEQLQRSYEDRKFGISGWEFDSENKLVIIYAYSILDEKKVKAIQGRQAGGW
                     TFKVIHNLDYENERKQAAAELMQFQNDHPELEISGFIITSPAHVEMWVRNRTPENEAL
                     NGTVMYGRTIELIGGAPREELMMREELKWNEPASREPEAG"
     gene            complement(3577..4185)
                     /locus_tag="PLI76_09915"
     CDS             complement(3577..4185)
                     /locus_tag="PLI76_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62389.1"
                     /translation="MTQEIRSLNQADTQIPAANRHIRQDRDCHSSAGAYLPFMIGYYR
                     HGLSDCGGGLGPWNSADCLPNVMIRYARTRKCLAHLQTLAGCYWMEIDGCPEHCYIEG
                     TFNLDFYMVSLINNSREFGHVICAEFLGSDMQTFSSWKFYQFDNLDIRPGNWQMPYGT
                     ETEDTKVKIYEITGIFNCGLPDHDLVPLVTFLIKENGGVTKG"
BASE COUNT          875 a         1333 c         1275 g         1009 t
ORIGIN      
        1 caaccttcga cccctcggtc ctcggggtct tcgtcttcgg cctcgtcgcc ggcatttgcc
       61 cctgcaacag cgtcctttgt cttgggctga tcggctacct gacgagcgga aagacggatc
      121 ttacgcttgc cgggatcctc aggctgacca tagcgttctc gttcggaacc atcctcgtcc
      181 tcctcccgct cggggttata gcggggtacc tcggggagta cctcctcttc ctcgacgaga
      241 cgatcgcgtg ggctatcggc ggggtgctga tgatcctgat ggggctgcag ctcctgcatg
      301 cgtataaacc gccgatacgg aggatattca actttttccg ggcgcctatc tcctactccg
      361 tcacgggcgc gttcctgctc gggttgtcgt tcggagcgat caccgtgggg cgcggggcgc
      421 cgatgcttct tattgtcctg acctacatcg ccctctacca gacggctatc gagggactct
      481 tcacgatctt cgtgtatgcc gtggggctct cgatcccgct catcgtgctc agctcggtcg
      541 ggggcgcggt cgggaagaag gtcaaggaga aggcgcggat gagcggggaa gtctttgacc
      601 ggatcatcgg catcgcgatc gtcctgatcg gggtctactt cctgactctc gcgttcttct
      661 gagggttcac tcctcctcgg gccggctttc cccacggggg cccggatcga gggcggcgag
      721 cacccggaga acaacgtaaa ggatcgcggc gacgagcacg cactcgaggt agaacccgaa
      781 gactgcgaac gcggagaaga agagtttata cgtgggcgta tctgcggcga gcggcaggac
      841 actcatgatg ccgtcgaaga accacccgaa gatcggcacg ctctccgcta tgaggggagc
      901 aggcccgccc ggccaccgct cggggtttat caccagggcg ccgacgaacg ggaccgaggc
      961 tgcgatggcg acgagcattg cctgccgttg ccttttgacc gggacggcgc cctcgatcag
     1021 ggggagcggt gttacggccc ccgcgaccac cagggcgacg atcacgagga tgaagacggg
     1081 gaggacgaac ggtggggccg ggcccggcgt tccggagacg atccttgcgg tacctgccca
     1141 gataacggcc gtaacggccg cgacgagcgg agggagtgcc ggcgagaacc ggcttcgggg
     1201 gagggggtgg atgatgttac ctgacatatt gcaccgtccg ggatgcggtt ttgctgccgg
     1261 gctcggtatt agttgatgaa tggtcggcgc atccccataa atagcctctg ccatgattat
     1321 ttacacattg gagtgtttcg atcgattttc tctcgatgtt acggaggggc cgggatacat
     1381 ggccccctcc ctcatgagcg gtcatcggat atccatccgc atgaacttcg tatacccgat
     1441 agcgaagaat atcacagggt agatggtcag cgccgcaata ctcgaccata tcctgccgag
     1501 catatcctcg agggtcgccc cggaggagtt gctgatcccc tggatgaggg cgttggtcgc
     1561 catttgtggt gatacgaggt tcgtgatgct ctggatcagg ttgaattgcc cgtaatatgc
     1621 cgtgatcgct gcctggaatt cttctgacga gatatccccg ccgtatgcgg cagggtcggg
     1681 ctcttccatc atgaacgcca tgccgatatg cgtggtggcg taaggggcca gaagaacgag
     1741 gaagatgaag acgatcatcg aaagaagcag ggcgctgccg ctctcccggc agagggtgga
     1801 gatggcaagc gcgatggaga agaaggtcac gaagaagaga agggtgacgc cggcataggt
     1861 caggaccatc cagaggtcgc ccggggcggg cacgatcgag aagacgagga ggagcgcggt
     1921 ggatatggcg agcactcctc cgacgacgag ggccagcagt gcgatgcccc cgagcgcctt
     1981 gccgttgatg atctcgtcgc ggtagaccgg gtgagagaga atgcttttga gtgaccggga
     2041 ctccttctct ctggagacca ggtcgaaccc gacggcgatg gcgaggaggc ctccgcaaga
     2101 gatcagcgtg agaaacatcg aggagtaaat atccgcgacc ggcggttttg tgggcatcat
     2161 tcgttccggt ccgtcgaacc tgtcgtccat cgcggccagt tgctcggagt atctatccag
     2221 ttctctctcg tagctcacga ccccgctgta cgtccccacc agggcgatgg cgaggaacag
     2281 ggcgaggatc accaggaacc gccagcccgt gacctggtcg gtgaattctt tccgcgccac
     2341 aatgaagacc cgttctgtct tcatcccccg ggaaacccga tccatctgca cttttcccga
     2401 taccatcgat tctgttgcca tcctgttatc ctcgtaaaga cccccgcctg gagcaccgcc
     2461 gatgggtctc agacctggaa aggccgcccg gacatcccca tccagagact gggccgtaac
     2521 tctatttacg ttttctctca cgttcctcta cacgtctgcc gccgtcttgc ggaaagtggg
     2581 cggtgaggtc cttcctgtgt ggcagaaaaa acccggatct tcaagggttg ccccggggag
     2641 gtgagtgctc cggataagcc atcgacggcc acctgcagcg aggaacgggt tgtggaactc
     2701 ttaatatacg cactaacaat aaccggtccc ctcccctcac ggggcctggt gtcgaccacc
     2761 cgaactctgg ctttcgctct gcgcctcaac ccgcctccgg ttcccgcgat gcaggttcgt
     2821 tccatttcag ttcctcccgc atcattagtt cttcccttgg tgcgcctcca attaactcaa
     2881 tcgtcctacc gtacatcacg gtgccgttca atgcctcgtt ttcgggtgta cggttgcgca
     2941 cccacatttc cacatgggcg ggtgatgtta taataaaacc cgatatctcc aattcaggat
     3001 gatcattctg aaattgcatc agttcagccg cagcctgctt acgctcattc tcataatcga
     3061 gattatggat gaccttgaat gtccagccgc cagcctgcct gccctgaatg gccttcacct
     3121 tcttttcgtc cagtatggag tacgcataga tgatgaccag tttgttctct gagtcaaact
     3181 cccatccgct tataccaaac tttctgtctt cataacttcg ttgcagttgt tcaatctcct
     3241 ctgtcagctc ttccggcaac acctccgccg tcatatttgc ctcttctagc gcttgcctca
     3301 cgaacggatg cacatatagt ccctgcgatg atctttccgg tgtcatgttc ctaacctcct
     3361 gcatttcttc aggtacctca tttggcgttg cattcgagct tataccgccg ggtaagtccg
     3421 accctagtgg cgatccctcc ggcgtcacgt tcccgacctc cggatgggga taactgtccg
     3481 gaagatgcac tgtcacgatc atcgctcccg tgcaacagac gattgcaagt gcgatgatga
     3541 tccgtagggt cgcttttgtc atttattcct cctctctcaa cccttggtta cacccccatt
     3601 ttccttgatc agaaacgtca ccagaggcac caaatcgtgg tctgggagac cgcaattaaa
     3661 gatgcccgtt atctcgtaga tcttcacctt tgtatcctct gtttccgtcc cgtagggcat
     3721 ctgccagttc ccgggcctga tgtccaggtt gtcaaactgg taaaacttcc agctggagaa
     3781 cgtctgcata tcgctgccta agaactcggc acagatcaca tgaccaaact cccgggagtt
     3841 gtttatgagc gacaccatgt agaaatccag gttgaaggtc ccctcgatgt agcaatgctc
     3901 ggggcagccg tctatctcca tccagtagca gcccgccagt gtctggagat gtgcgaggca
     3961 tttccgggtc cgggcatacc gtatcatcac gttcggcagg caatcggcac tgttccacgg
     4021 gcccaaacct cccccgcagt ccgaaagccc gtggcggtaa tacccgatca tgaagggcag
     4081 atacgcgcct gccgacgagt ggcaatccct atcttggcgg atgtgccggt tggcggcagg
     4141 aatttgagta tcagcctgat tgagtgatct tatttcctgt gtcatgcgtg ttcatccagt
     4201 cttcgaaacg atgcccggag gaacgctaga ccacaacgac tatccctgat ggcagcctac
     4261 agccctattg gccctccggg cacgcttttg gggggcggga tacgacggca atcggggttc
     4321 ccggcccccg cgggataccg tacacggtgt caccgatcaa ggcgtcaacg ttcccccata
     4381 taccttttct gtgacaaaca tcgaactgtc gcggttgtac cctggccgtc gcattctcct
     4441 gatacgccct cgttgttcca tctcccggca gtactgccga tatccagtgg aa
//
LOCUS       DAOSEE010000160         4464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_6869_length_4464_cov_228.012248, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000160 DAOSEE010000000
VERSION     DAOSEE010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6869_length_4464_cov_228.012248"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2784
                     /locus_tag="PLI76_09920"
     CDS             <1..2784
                     /locus_tag="PLI76_09920"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HOI62390.1"
                     /translation="VLITHMDPAQKGLLPEILRRFTRMPVRETEDGMKVEANTVYVKP
                     AQADLTLFHGTIGHLEPVRTHGLRMPIDSFFRHLADDQDGKAVGIVLSGTGSDGTLGI
                     RAIKERMGMVMVEDPSSAQFSGMPRSAMATGLVDYSAPPEELPELLIQYVKFSATTPT
                     GEKLTTARVEADLRRIFSLIRLRNGQDFSRYKRNTIRRRIERRMALHQIPSIGEYVTF
                     LYENPPEVDTLARELLIGVTRFFRDQKAWVALKEGILKDLIPLRPEGGTIRAWSVGCS
                     TGEEAYSLAIVLRECLDTLKRPDMVQVFATDVDARAIGIARRGTYPANITADVSPERL
                     ERFFVKEDNTYLVHKEIRESVVFATQNVLADPPFTHLDIISCRNLLIYLSAELQKQLI
                     PLFQYALNPGGFLFLGTAESVGGFNTLFKTVDARWKIFRRQEGTTPQVPRFEIPAAVV
                     AQPATAEKKAREFAREDEIVTALAREWLLVQYAPPAVIVNEDGDILYFHGRTGKYFEP
                     RPGRATLNIHTMARPGIRDALISLLQAATREREGFVQEAAPARVSDGEQQILITIRPV
                     GQLAGRGGMLYVVVFQNNEKPYRKYPEGGAEERKQSDRAPEGGTVQEIARVRFQIQHT
                     IEDMQASQEELKSMNEELQSTNEELQSMNEELTSSREELQSLNEELLTVNMEHQKKIA
                     ELSESNDDMRNLLQITNIAMLFLDDDLRVRRFTTPIRPIVNLQAGDVERPITDLAVNL
                     KEGGFSDDLRGVLRTLQVREREVQTKEGRWFKMQIRPYRTAENRIEGVIVTFIDISQS
                     RDLESSIRRARACAEEVMAAVHEPLLVLDDNHRVVSANRSFYTMFGVTREEAEGNLIY
                     ALQDRQWDIPRLHELLEEILPKQGEIEDFLVEHDFPGVGHRVMRFNARALPPDAGPDR
                     ILLAVGGSIDRPI"
     gene            2946..4325
                     /locus_tag="PLI76_09925"
     CDS             2946..4325
                     /locus_tag="PLI76_09925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI62391.1"
                     /translation="MTQHTKEECARQLQAIFQENDKDPAALRAAVQDFFRRLAADWSR
                     TIEDLRRENVLLQEARQCLCQDTHAEALERIQTYAEELATANEELETANEMLNNRNEE
                     LIAARKVAEAERERYYELFDAAPEGYLVTDRNGKVLEANRTAGVLLAVHKESLIGRNL
                     LSFARNENRVNLKAWMVQAVKGQGRQTFEADLLPCMEEPVHVSVTIVAVRGGEEGEPS
                     LRWMVQDISGRKRAEEALRRLNREVEAARDEANLYVDILTHDIRNTENVSNLYAELLI
                     DCLEGDEAQYAANLQRSIRKSIDIVGTISTIRQIHQTGSELRPVDLDAAIRDTVKGYP
                     RTIIHYVRTDRQVLADDLLPVIFNNLIGNAVKHGGPGVEIAIRAEEEDGFVRVSVEDN
                     GPGVPDEEKDAIFRRYEQQKRGVGAGLGLHLVQILIRRYGGRIWVEDRIPGTPGAGTA
                     FRLTLRKAA"
BASE COUNT          945 a         1363 c         1443 g          713 t
ORIGIN      
        1 gtgctgatca cgcacatgga tcctgcccag aaagggctcc tgccggagat cctccggcgg
       61 ttcaccagga tgcccgtgcg ggagaccgag gacggcatga aggtcgaggc gaacacggtg
      121 tacgtgaaac ccgcacaggc ggacctcact ctattccacg gcaccatcgg gcacctggaa
      181 ccggtccgga cgcacggtct ccggatgccc atcgacagtt tcttccggca ccttgccgac
      241 gaccaggatg gaaaggcggt agggatcgtg ctctccggga cggggagcga cggaacgctc
      301 gggatcaggg ccatcaaaga gcgcatgggc atggtgatgg tcgaggaccc gtcttcggcc
      361 cagttcagcg gcatgcccag aagcgcgatg gccaccgggc tcgtggacta cagcgctccg
      421 cccgaagaac ttcccgagct gctcatccag tacgtgaaat tctccgctac gactcccacc
      481 ggggagaagc tcaccacggc gcgggtggaa gcggacctcc ggcggatatt ctctctcatc
      541 cgcctgcgaa acggccagga tttctcccgg tacaagcgaa acaccatccg aaggcgtatc
      601 gagcgccgga tggccctgca ccagatcccg agcatcgggg agtacgtcac gttcctctat
      661 gagaaccccc cggaggtcga tacccttgca cgcgaactcc ttatcggtgt gacgcggttc
      721 ttccgtgacc agaaagcctg ggttgctctg aaagagggga tcctcaaaga cctgatcccc
      781 ttgcggccgg agggagggac gatccgggcc tggagcgtgg gctgctccac cggggaggaa
      841 gcttacagcc tcgccatcgt gctcagggag tgcctggata ccctgaagcg gccggacatg
      901 gtccaggtct ttgccaccga cgtggacgca agggctatcg ggatcgcgcg gcgcggcacc
      961 tacccggcga acatcaccgc cgatgtctcc cccgaacggc tggaacgttt cttcgtaaag
     1021 gaggacaaca cctacctcgt gcataaggag atccgcgaga gcgtggtctt tgcgacgcag
     1081 aacgtcctcg cagacccccc gttcacgcac ctcgacataa tctcctgccg taacctcctc
     1141 atctacctct cggcggaact gcagaagcag ttgatacccc tcttccagta tgccttgaac
     1201 ccgggcgggt ttctcttcct cggcacggcc gagtcggtcg gcggattcaa taccctgttc
     1261 aagacggtcg atgccaggtg gaagatcttc cggcgccagg agggcaccac cccgcaggtg
     1321 cccaggttcg agataccggc ggccgtagtc gcgcaacccg caacagcgga gaagaaggcg
     1381 cgagagttcg caagggagga tgagatcgtc accgcccttg cccgggaatg gctgctcgtg
     1441 cagtatgccc ccccggcggt gatcgtgaac gaggacggcg atatcctgta cttccacggc
     1501 aggacgggga agtactttga accccggcct ggcagggcta ccctgaacat ccacacgatg
     1561 gcacggcccg gtatccggga tgcgctgata tccctcctcc aggcggccac ccgggagaga
     1621 gaggggttcg tccaggaggc agctcccgcg cgggtgagcg acggcgagca gcagatcctg
     1681 atcacgattc ggccggtcgg gcagctggcg ggaagaggcg ggatgctcta cgtcgtcgtc
     1741 ttccagaaca atgagaaacc ataccgaaaa taccctgaag gcggggcgga ggagcgcaaa
     1801 caatcagacc gtgccccgga agggggcacg gtgcaggaga ttgcccgggt ccggttccag
     1861 atccagcaca cgatcgagga tatgcaggcc tcgcaggagg agctcaagtc gatgaacgag
     1921 gagctccaga gcacgaacga agagctccag agcatgaacg aggaactgac gagttcccgc
     1981 gaggaactcc agtccttgaa cgaggagttg ctcaccgtca acatggaaca ccagaagaag
     2041 atcgcggaac tctcggagag caacgacgac atgcgcaacc tcctgcagat aaccaatatc
     2101 gccatgctct tcctcgacga cgatctgcgg gtgcggcggt tcaccacccc catccggccc
     2161 atcgtcaacc ttcaggcggg ggatgtagag aggcccatca ccgatcttgc cgtcaacctc
     2221 aaagaggggg ggttctcaga cgatctccgc ggcgtgctca ggaccctgca ggtgcgggag
     2281 agagaggtgc agacgaagga ggggagatgg tttaagatgc agatccggcc ctaccgaaca
     2341 gcggagaacc gtatcgaggg cgtgatcgtc accttcatcg atatcagcca gtcgagggat
     2401 ctcgagtcgt ctatcaggcg ggcacgcgca tgcgctgaag aggtcatggc cgccgttcac
     2461 gagcccctcc tggtgctgga cgataaccat cgtgttgtct ccgcgaaccg ctcgttttat
     2521 acgatgttcg gggtcacgcg ggaggaggcc gagggaaacc tcatatacgc gctccaggac
     2581 cgccaatggg acatcccccg gctccatgaa ctgctcgagg agatcctgcc caaacagggt
     2641 gagatcgagg attttctggt ggagcacgac ttcccgggcg tcggccaccg ggttatgcgc
     2701 ttcaacgccc gggcgcttcc cccggatgcc gggcccgacc ggatcctcct tgccgtcggc
     2761 ggttcgatag atcggccgat ataggtaccg gaagtgccca aacctgaagc ggggcctgtt
     2821 cctgcggggg accgatcgaa gggcaatcgg cagatatatg agtttattgg tgtaacggtt
     2881 ggaatacagc tgccggtctc cggccgcggg gttccagcag ggatcagacc atcatgagat
     2941 ccggtttgac gcaacatacg aaggaagagt gcgcacgaca gctgcaggcg atatttcagg
     3001 agaacgataa agaccccgcc gcactccgtg ctgccgtgca ggacttcttc aggagactgg
     3061 cggcggactg gtcccggacc atcgaggatc tccgacggga gaacgttctg cttcaggagg
     3121 cccggcaatg cctctgccag gacacccacg cagaagccct ggagcggatc cagacctatg
     3181 ctgaggaact tgctacggca aacgaggagc tcgagaccgc aaacgagatg ttgaacaacc
     3241 ggaacgagga gctgatcgct gcccggaagg ttgccgaggc ggaacgggag cgctactacg
     3301 agctcttcga tgccgccccc gaaggctacc tggtgacgga caggaacggg aaggtcctcg
     3361 aggccaaccg caccgcgggg gtgctcctcg ccgtccacaa agagagcctg atcgggcgaa
     3421 acctcctcag ctttgcccga aatgagaacc gggtaaatct caaggcctgg atggtccagg
     3481 cggtgaaggg gcaggggcgg cagaccttcg aggcggatct cctgccgtgc atggaggagc
     3541 ccgtgcatgt ctccgtgacc atcgtcgcgg tcagaggggg agaagaggga gagccgtcgc
     3601 tccgctggat ggtccaggat atcagcggcc gcaagcgggc cgaggaggcg ctccggcgcc
     3661 tgaaccggga ggtcgaggcg gcacgcgacg aggcaaacct ctacgtggat atactgaccc
     3721 acgacatacg gaacaccgag aacgtctcga acctctacgc cgaactgctc atcgactgcc
     3781 tggagggaga tgaggcccaa tacgcggcga acctccagcg gagcatcaga aagagcatcg
     3841 atatcgtcgg caccatctcc acgatccgcc agatccacca gacgggttcc gagctcaggc
     3901 cggtggatct cgatgcggcc atccgggata cggtcaaagg ctatccgcgc accatcatcc
     3961 attacgtaag gacggatcgc caggtcctcg ccgacgacct gctccccgtg atcttcaaca
     4021 acctcatcgg caacgcggtg aaacacggcg gtcccggcgt cgagatcgcc atccgggcgg
     4081 aggaagagga cgggtttgtc cgggtctcgg tcgaggacaa cggccccggc gtgccggatg
     4141 aggagaaaga cgcaatcttc cggcgctacg aacagcagaa acggggcgtc ggggcggggc
     4201 tcggcctcca tctcgtgcag atcctcatcc gccgctacgg cggcaggatc tgggtcgagg
     4261 atcgcatacc gggcaccccg ggtgcgggca cggcgttccg gctgacgctc aggaaggcgg
     4321 cataggcgca cggcccgggg gtgttctgcc ggcagagcgc ctctgccgcg gagttccggc
     4381 gaaaggcgcg tagcgtgtac taccgccgga acccggagat gctcttttac ctgatcaagt
     4441 gccgggcccg ggacgacgga gcgg
//
LOCUS       DAOSEE010000161         4424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_6943_length_4424_cov_239.598764, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000161 DAOSEE010000000
VERSION     DAOSEE010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6943_length_4424_cov_239.598764"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PLI76_09930"
     CDS             <1..194
                     /locus_tag="PLI76_09930"
                     /inference="COORDINATES: protein motif:HMM:NF012643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62392.1"
                     /translation="AALEDVNIEEAISKAAVAPVAAAPVAAAAPAAAAPAAEEEAEEE
                     SKKEEEEESGMAGLGALFG"
     gene            573..941
                     /locus_tag="PLI76_09935"
     CDS             573..941
                     /locus_tag="PLI76_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI62393.1"
                     /translation="MSKKVMIIDDNEKFLRITGLLIQSFGYTLITLNDPREGLEMISR
                     DPPSLILLDIMMSPMDGSQFLDERRKIPGALDIPVVICSAWRLTEEELAPYRNEIVGV
                     IAKPVEPALLREILKDHLGV"
     gene            1011..3926
                     /locus_tag="PLI76_09940"
     CDS             1011..3926
                     /locus_tag="PLI76_09940"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOI62394.1"
                     /translation="MPVNFTAPDIKRLVSKRDLGGLITAIGSEDKDTRWMAAGGLGEL
                     CDPRAIEPLIRALADVDPDVRWKAAEALGSIGDTRATEVLIPLLRDPDGIMRLQAVWA
                     LGKIRDQSATAHIIPFLTDPDYDVRIAAVWALGAIGGGRAVAALREMLLDRHPGIRSK
                     AAESLEKCGWKPVDDRERGAFAFARRDWDEVSHYGRRLEDILIWALDDRYFDVRMHAA
                     RILGTTRSRSAIPHLHKALDDPEECVTYEAAAALAEIGNAESKKALVHGLESPHLSTR
                     KVAAGALDRLAWQPRSIYHESLFLSAKDDWIGLVRLKKHGVDPLARELQERCGVERAS
                     IAKALGAIGSPAIDAMIGLLGSPNPDIRWRAASILGDSRDRRAVEPLIAALADPDEHV
                     SSSAAVALGEIRDEGAVDPLIRAYATGGPGLRRDIVAALGRTRSERAVAAIIAASGDE
                     NPVVRSSAVRAMGRIRDVRVLPALIPFFQDPDPAIRFEAVVAIRPYSGREIDLLLTRA
                     LEDPDAGVRREAARQAGSRGVTTAVQPLVGLFGDPDAAVRKAAARALERIGWEPSDSR
                     ELLAVLIAKEDWEEIQRQGLLYSPAGDPKSGGAGLLPGGLQGREGVVSPGPADQEKQI
                     PQEEREVSPRRGVNVATHEAVPDLQYFIDILNDPRVDLPARLKAVEALGNLGDLRGVR
                     PLMQALRDPESEIRGNAALSLGMLGDHKAFGSLVVALEDPVFEVKRRVAEALSVLKPG
                     GAVLPLAELARSPNPGSRMLAVTVLGDFEEDDGVRALLGALNDEYSGVKSAALSSLLK
                     LVDYWGSRIPVFLKDGDPAIRKNVLTALNTIIGEDKAISLLIPLLHAGNFSVRREAVL
                     ALEGMGWRPGLPEEHAMVLIAGREWDEVVILGKQAESALIGALFDTDREIRDGAVASL
                     GMIGDDETVRSIRAAMRGGVDLQARGIHAAMKAASLIEEKEAGRGGEGLIPSSPGVR"
     gene            complement(3996..>4424)
                     /locus_tag="PLI76_09945"
     CDS             complement(3996..>4424)
                     /locus_tag="PLI76_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOI62395.1"
                     /translation="IAPTAAFTAHVVIGPAPLAVQFTDLSTGNPTAWIWTFGDGATSI
                     EQHPVHTYGTPGTYTVNLTVSTMSSSASLSRPDYITVTRKGDLNGDGDVDIGDVSNVA
                     YMVVGKAPEDPAADFNGNGKVDIGDAAKIACFIVGKIGEL"
BASE COUNT          810 a         1200 c         1531 g          883 t
ORIGIN      
        1 tcgccgcact cgaggacgtg aacatcgagg aggccatcag caaggccgcc gtcgctccgg
       61 tcgccgccgc acctgtggca gccgccgccc ctgccgccgc cgcgcccgcg gccgaggaag
      121 aggcggaaga agagtccaag aaggaagagg aagaagagag cggcatggcc ggtctcggcg
      181 ccctcttcgg gtaatctttt ttttcggttt gtttgcgatt tttgcggcgt gattttcccg
      241 ggagccacgg ccggacgggt tttgggaata catatgatgg ggccgagagc atatccggca
      301 atccgtgatg gttcgtgttg ggtccgggcc ggtgggcacc ggcgtatcat cgacaaagtt
      361 gccgtatccg tcggacccct gttctgcaga ttccggcagg tgaacgcctg gttaatcata
      421 atttcatcga ttttcccctt ccggtcgtgt atgttcgtcc gatgtatata gataagtata
      481 aattatcctt tggatgattg ttcattcaag accggatccc ctgcagggcc gcagtcgctc
      541 gccccggcag gaatcaggga ggtaaatcgg gtatgtcgaa gaaagtgatg ataatcgacg
      601 acaacgaaaa attcctccga atcaccgggc tgcttataca atcatttgga tacaccctca
      661 tcacgctcaa tgacccgcga gaggggcttg agatgatctc aagagacccc ccctcgctga
      721 tactcctcga tatcatgatg tcgccaatgg atggttcgca gttcctggac gagcggagga
      781 agatccccgg agcgctggat ataccggtgg tgatctgttc ggcgtggagg ctgaccgagg
      841 aggaacttgc cccttatagg aatgagatag tcggggtcat cgccaaaccc gttgaacccg
      901 ctctattgag ggagatcctt aaagaccatc tcggggtgta atggcgtgaa gctcggaaag
      961 gtttgttaca ggcgcctacc ggaaggatct ttttcccggg gtggagtata atgccggtta
     1021 acttcaccgc ccccgacatc aagaggctcg tctcaaaacg ggatctcggc ggtcttataa
     1081 cggctatcgg gagcgaggat aaggatacgc ggtggatggc agcgggggga cttggggagt
     1141 tatgcgaccc gcgtgcgatt gaacctctta tccgggcgct ggccgatgtc gatccggatg
     1201 tcaggtggaa ggccgcggaa gcgctcggtt ctatcgggga tacccgggcg acagaagttc
     1261 tcatcccgct cctcagggac cctgatggga ttatgcgatt gcaggcggtc tgggcgctcg
     1321 gaaagatccg ggatcagagt gcgacggcgc atatcatccc cttcctgacc gatcccgact
     1381 acgacgtcag aatcgcagcg gtctgggcgc tcggggccat agggggcggg cgtgccgttg
     1441 ccgcactgcg ggagatgctg ctggatcggc acccgggcat ccggtcgaag gcagctgaat
     1501 ccctggagaa gtgcgggtgg aaaccggttg acgaccgtga gcggggtgct tttgcatttg
     1561 cacgaaggga ttgggatgaa gtgtctcact atggtcggag gctggaggat atcctgatct
     1621 gggcgctgga cgaccgttat tttgatgtcc ggatgcatgc cgcaaggatc cttggtacaa
     1681 cgagaagccg gagcgctatc ccgcacctcc ataaggccct tgacgacccg gaagagtgcg
     1741 tcacttatga ggcggcagcc gcacttgcgg agatcgggaa cgccgaatca aagaaggctc
     1801 ttgttcatgg gcttgagagc ccgcatttaa gtacccggaa ggtcgcggca ggggcactcg
     1861 atcgcctggc atggcagcca cggagcatct accatgagag cctgttcctg agcgcaaagg
     1921 atgactggat cggtcttgtg cggctcaaga aacacggcgt cgatcccctc gcccgggagc
     1981 tgcaggagcg atgcggtgtg gagagggcga gcatcgcaaa ggcgcttggg gctatcggca
     2041 gcccggcgat cgatgccatg atcgggttgc ttggaagccc gaaccccgat ataaggtggc
     2101 gggcagcctc gatactggga gatagccgcg atcggcgggc ggtcgagccg ctcattgcgg
     2161 ccctggcaga tcccgatgag cacgtgtcat cttccgccgc ggttgccctc ggtgagatcc
     2221 gtgacgaggg ggcggtggat cccctcatca gggcgtatgc aaccggaggc ccgggtctcc
     2281 gccgcgatat tgtcgcggcg cttgggagaa caagatcgga acgggccgtt gcggctatca
     2341 tcgcagcatc cggcgatgaa aatcccgttg tccgcagtag cgccgtgagg gccatgggcc
     2401 ggatcaggga tgtgcgggtt ctccccgccc tgatcccgtt cttccaggat cccgatcctg
     2461 ccatccggtt tgaggcggta gtggcgattc gcccgtattc cggccgggag atcgatctcc
     2521 tcctcacccg ggccctcgaa gacccggatg ccggggtacg acgggaggcg gcccggcagg
     2581 ccggcagtcg tggggtgaca accgcggttc aaccgcttgt cgggctcttt ggagacccgg
     2641 atgccgcggt gaggaaggcg gccgcaaggg cgcttgagag gattggctgg gagccttcgg
     2701 attcccggga gctgttggcc gtcctgattg caaaagagga ctgggaggag atccagcgcc
     2761 agggccttct ctactctcct gccggggacc cgaagagcgg cggcgcgggg ttgctgcccg
     2821 gtggcctgca gggcagggag ggagtggtct ctcccggccc cgccgatcag gagaagcaaa
     2881 tcccgcagga ggaacgtgag gtctctccac ggcggggggt gaatgtcgca actcacgaag
     2941 ctgttccaga tctgcagtat ttcatcgaca tcctcaacga ccctcgcgtg gatctgccgg
     3001 cccggctcaa ggctgtcgag gcgctcggga atctcgggga tctcagggga gttcgaccgt
     3061 tgatgcaggc tctacgcgat ccggaatcgg agatccgcgg aaacgcggcc ctctctcttg
     3121 gtatgctggg cgaccataag gcctttggtt ccctggtcgt tgcactcgag gatccggtgt
     3181 ttgaggtgaa gcggcgggtt gccgaggcgc tctcggttct gaaacccgga ggtgcggtcc
     3241 tcccgcttgc cgagctggcg aggagcccga accccggcag ccgcatgctt gccgtcacgg
     3301 tcctcggcga ctttgaagag gatgatgggg tgcgcgccct gcttggtgcg ctcaacgatg
     3361 agtattccgg ggtgaagtcg gctgcgctgt cgtcgttgct caaactggtc gactactggg
     3421 ggtcacgtat accggttttc ctgaaagacg gggatccggc catccggaaa aacgttctga
     3481 ctgctctgaa taccataata ggggaggata aggccatttc tctgcttatc ccgctcctcc
     3541 atgccggcaa cttctccgtc cggcgagagg cggttctcgc gctcgagggg atgggctgga
     3601 ggccgggcct gccggaagag catgcaatgg tgctgatcgc cggcagggag tgggatgagg
     3661 ttgttatact tggaaagcag gcggagagtg ccctgatcgg cgctctcttc gatacggacc
     3721 gggagatccg ggatggggct gtggcgtctc tcgggatgat cggggatgat gagacggtcc
     3781 ggtcgatcag ggcggcgatg agggggggtg tggatctcca ggccaggggg atccatgccg
     3841 cgatgaaggc ggcgagcctc atcgaggaga aagaggcggg gagaggcggg gaggggctaa
     3901 tcccctcttc tcccggtgtt cggtgaggtg tttgccggcg agggtcgtcg cgcaatttcg
     3961 tgagccggta tgtggggctc cggggagatc agggattaga gttcccctat cttcccgacg
     4021 atgaaacagg cgatcttcgc cgcgtcgccg atatcgacct tcccgttccc gttgaagtcg
     4081 gcggcgggat cttccggggc ttttccgacg accatgtagg ctacgtttga gacgtcgccg
     4141 atatcgacgt caccgtcgcc gttgagatcg cccttacggg tgacggtgat gtagtccggc
     4201 cgggagagcg aggcggaact gctcatggtg ctgaccgtga ggttcacggt gtaggtgccc
     4261 ggggttccgt aggtgtggac ggggtgctgc tcgatggagg tcgcgccgtc cccgaacgtc
     4321 cagatccacg ccgtcgggtt gccggtcgag aggtcggtga actgcaccgc gagcggggca
     4381 gggccgatga cgacgtgcgc ggtgaatgcc gctgtcgggg cgat
//
LOCUS       DAOSEE010000162         4409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_6984_length_4409_cov_220.426275, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000162 DAOSEE010000000
VERSION     DAOSEE010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6984_length_4409_cov_220.426275"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..3682
                     /locus_tag="PLI76_09950"
     CDS             59..3682
                     /locus_tag="PLI76_09950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOI62396.1"
                     /translation="MAASTPVQALYVDDEAVLLDIARLYLERTGDIAVDITANPLEAY
                     TLIMTGRYDVVVSDYQMPEMDGIALLKRAREAGSQVPFIIFTGKGREEVVIEALNNGA
                     DFYLQKGGDPKTQFAELANAIRQLAGKQKAEIAVRRSEEMLHKAESLAHLGSWVFDHR
                     TGRLTWSDETYRIFGLDPGERVMTHESFLSMVHPDDRAMVHATYSSSVTKGEASYTVE
                     HRIFRADTGEVRYVAERCEHLRDALGEATLSVGMTHDITERKLAELELLQRHEELRAA
                     YEQLASIEEELRTSFDDLAGSQRRIQESEAKLADIIDFLPDATFVIDTDGCVIAWNRA
                     MEKMTGIPKAEMLGRGDYAYAVPFYGEACPVLIDYVLHPGREADSPYQTEKISEDILD
                     GETSRARLAGRAVALKIRASPLYDREGRCVGAIEIVRDITGQKQVEADLRETKKYLEN
                     LIDHASAAIVVWNPECTITRFNQAFEHLTGIPADEAIGQKVEILFPAKSRDALMERIR
                     QAMAGGSWRDAEVPILHRSGSVRTVLWNTANITGNDGETLVSTIAQGQDITFRKEAET
                     KLLETHRELLAAYEQIASNEEELRRSFDDLAESQRQLQQSEDLYRRISGSISDIVYAC
                     DLQDTGGYALSLIAGAVSRITGYTAEEVLAMRCWKALVHPDDHSVFERDIVPLSPGMS
                     SVTELRIIHRDGSVRWIRVTANCTTGQDGCPRMYGGWQDVTAQKKAEEALRESEQHFR
                     TLADSGQALIWACGLDAGWDYFNEPWLAFTGRTLEEEIGDGWMEGVHPDDRSRCIEIF
                     TGAFARRKPFSMTHRLRRYDGEYRWVQDDGSPRYDTRDNFLGFIGHCLDVTELRRMEE
                     MLQETLEMMEGIFLVAPTGIGMTVGGVFTEANLQLCEITGYTRDELIGKPMRLLYPGE
                     TEYETALEVMRAQIKMTGRCTIETRFLRKNGTSSDVQLAIALFDPADLSRGMALTVLD
                     ITESKAMEREIESHARELLRQTSSLAAVNKKLNLMNSITRHDVLNQLTILLGNLSFAQ
                     EARPGQDISRFLSPVKDAAGRIQRQIEFTRDYADLGIGEPEWQRISDAIRSAAYEFPT
                     EDESGDLAIYADPMLARVFANLMDNTIRHGGQVSRVRVRYWLEGDGALTLAWEDDGIG
                     VPAKEKEEIFKQGIGKNTGLGLFLIREILGITGIAITETGEPGKGARFEMRVPEGMYR
                     FGE"
     gene            3726..4172
                     /locus_tag="PLI76_09955"
     CDS             3726..4172
                     /locus_tag="PLI76_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3887 domain-containing protein"
                     /protein_id="HOI62397.1"
                     /translation="MNLPRLSLFVVLAVLLGISTAGCMDRETVLSGDDAAEVLAYAEP
                     IVDNLMQGFNEGNYAKYSRDFGPEMKQALDEAAFAENHEFVTSRIGLHESRGDPVVTK
                     TGEYVAVTYRAKFEREDGVALQFVFEKDDPSHRLQGLWFDSPMLRS"
BASE COUNT          908 a         1317 c         1406 g          778 t
ORIGIN      
        1 caaattcgaa aggtttaagg attgccctac tacatctaca cgtaggtatc aaccgtatat
       61 ggccgcatca acccccgttc aggcactcta tgtcgacgac gaggccgtgc tgctcgatat
      121 cgcccgcctc taccttgagc gaacgggcga tatagccgtc gatatcacgg caaacccgct
      181 tgaagcgtat acgctgatca tgaccggccg gtatgacgtg gtcgtctcgg actaccagat
      241 gccggagatg gacggtatcg ctctcctgaa gcgggcccgg gaggccggat cgcaggtgcc
      301 cttcatcata ttcaccggca agggacgcga agaggtggtg atcgaggcgc tgaacaacgg
      361 cgccgacttc tacctccaga aggggggcga tccgaagacg cagtttgccg agcttgcaaa
      421 cgctatccgg cagctggccg ggaagcagaa ggcggagatt gcggttcgcc ggagcgagga
      481 gatgctgcat aaggccgaga gccttgcgca tctcgggagc tgggtgttcg atcacaggac
      541 cgggcggttg acgtggtctg acgagaccta ccgcatattc ggcctcgatc ccggggagcg
      601 tgtgatgacc cacgagtcgt tcctctcgat ggtgcacccg gacgacaggg ccatggtcca
      661 tgcaacctac tcttcctctg tcacgaaagg agaggcctcc tataccgtcg aacaccggat
      721 atttcgtgcc gacaccgggg aggtccggta tgtcgctgaa cggtgcgaac acctgcggga
      781 cgcactgggg gaggccaccc tctcggtcgg gatgacgcac gatatcaccg agcgtaaact
      841 cgccgaactc gaactgctgc agaggcacga ggaactgcgg gcggcgtacg agcagcttgc
      901 ctccatcgag gaggagctcc ggacaagctt cgacgacctt gccgggagcc agcgccggat
      961 ccaggagagc gaggcgaagc ttgccgatat catcgatttt ctccccgatg caaccttcgt
     1021 catcgatacg gacggctgcg tcatcgcctg gaaccgcgcg atggagaaga tgaccggcat
     1081 accaaaggca gagatgctcg ggaggggcga ttatgcatat gcggtcccgt tctacggcga
     1141 ggcatgcccg gtcctgatcg attatgtcct ccatcccggg agagaagcag acagcccgta
     1201 ccagaccgag aagataagcg aagatatcct cgatggcgag acttctcgtg cccgtcttgc
     1261 cggaagggca gtcgccctca agatcagggc atcccctctc tatgaccggg agggccggtg
     1321 tgtcggcgca atcgagatag tccgggatat caccgggcag aaacaggtcg aggccgacct
     1381 gcgcgagacc aaaaagtatc tggagaacct gatcgatcac gccagcgccg cgatcgttgt
     1441 ctggaacccg gagtgcacca tcacccggtt caaccaggcc tttgagcacc tgaccggcat
     1501 acctgcagat gaagccatcg ggcagaaggt agagatcctc ttcccggcaa agagccggga
     1561 cgcgttgatg gagcggatcc ggcaggcgat ggcgggaggg tcgtggagag atgcggaggt
     1621 cccgatcctg caccggtcgg gcagcgtcag gacggtgctc tggaacacgg caaacatcac
     1681 cggcaacgat ggagagacgc tggtctcgac gattgcgcag ggacaggaca tcacgttccg
     1741 aaaagaggcg gagacgaaac ttctggagac gcacagggaa ctgctggcag cgtacgagca
     1801 gattgcctcc aacgaggagg aactccggag aagcttcgac gaccttgccg agagccagcg
     1861 ccagctccag cagagcgagg acctctaccg gcggatatcc gggagcatat ccgacattgt
     1921 ctacgcgtgc gacctgcagg atacgggcgg gtatgccctc agcctgattg ccggagcggt
     1981 atccaggatc accgggtata ccgcagaaga agttcttgcg atgcgatgct ggaaggctct
     2041 tgtccacccc gacgaccatt ccgtattcga gcgcgatatc gtcccgctct ccccgggtat
     2101 gtcgtcggtc accgagctcc ggataatcca ccgggacggt tcggtccggt ggatccgcgt
     2161 caccgccaac tgtaccaccg gtcaggatgg ttgcccgcgg atgtacggcg ggtggcagga
     2221 cgttaccgct cagaaaaagg ccgaagaggc gctgcgggag agcgaacagc acttccgcac
     2281 ccttgcggac agcggccagg ccctgatctg ggcgtgcggg ctggatgcag ggtgggatta
     2341 cttcaacgaa ccatggcttg ccttcaccgg ccggacgctt gaagaggaga tcggtgacgg
     2401 ctggatggag ggggtgcacc ccgacgatcg ctcgcgatgt atcgagatct tcacgggcgc
     2461 ctttgcccgg cgtaaaccgt tcagcatgac ccaccgcctg cgacggtacg acggagagta
     2521 ccgctgggtt caggacgacg gctccccccg gtacgacacc cgggacaact tcctcggttt
     2581 catcgggcac tgcctggacg tcaccgagct ccggaggatg gaggagatgc tgcaggagac
     2641 cctggagatg atggagggca tcttcctcgt cgccccaaca ggcatcggta tgacagtggg
     2701 cggggtgttt acggaggcga acctgcaact ctgcgagatc accggttata cgcgcgacga
     2761 actcatcggc aagcccatgc ggctgctcta tcccggggag accgagtacg agaccgccct
     2821 tgaggtgatg cgtgcgcaga tcaagatgac cgggagatgc acgattgaga cccgttttct
     2881 gcgaaagaac ggcacgagca gcgatgtcca gcttgctatc gcgctgtttg acccggcgga
     2941 cctctcccgg ggcatggcgc tcaccgtact ggatatcacc gagagcaagg cgatggagcg
     3001 ggagatcgaa tctcatgccc gggagcttct ccgtcagacg agcagtctgg ccgccgtgaa
     3061 caaaaaactc aacctgatga acagcatcac ccggcacgac gtcttgaacc agctgacgat
     3121 cctccttggc aacctctcgt ttgcacagga ggcacgaccc ggccaggata tatcgaggtt
     3181 cctctccccg gtgaaggacg ccgccgggcg gatacaacgc cagatcgagt tcacccggga
     3241 ctatgcggat ctcggtatcg gagaacctga gtggcagcgt atctccgatg ccatccgatc
     3301 ggcggcgtac gagttcccta cggaagatga gtccggagac cttgccatct acgcggatcc
     3361 gatgcttgca agggtctttg ccaacctgat ggacaacacg atccgtcacg gggggcaggt
     3421 gagccgggtc cgtgtccggt actggctgga gggggacgga gctcttaccc tggcctggga
     3481 ggacgacggc atcggcgttc ctgcaaaaga gaaggaggag atcttcaagc agggtatcgg
     3541 aaagaacacc ggacttggcc tcttcctgat ccgggagatc ctcggtatca ccggtatcgc
     3601 catcaccgag acgggagagc ccgggaaggg ggcacggttt gagatgcgcg tgcccgaggg
     3661 gatgtaccgg tttggggagt gaggccatct cctccattca tatcctcccg ggtcgatagt
     3721 accgcatgaa cctcccccga ctctcactct tcgtcgttct cgcggtcctt ctgggtattt
     3781 ctacagccgg ctgcatggac cgggagaccg tgctctccgg cgatgatgcg gcagaagtgc
     3841 tcgcatatgc cgaaccgatc gtcgataacc tcatgcaggg tttcaacgaa ggcaactatg
     3901 cgaagtactc ccgcgatttc ggccccgaga tgaagcaggc cctggacgag gccgcgtttg
     3961 cagagaacca cgagttcgtc acgtcccgta tcgggcttca tgagtcccgg ggcgaccccg
     4021 tcgtcacaaa gaccggcgag tacgtcgccg tgacctaccg tgcgaaattc gagcgggaag
     4081 acggcgtggc cctccaattt gtctttgaaa aggacgatcc gtcgcaccgg ctccagggcc
     4141 tctggtttga ctccccgatg ctgcgcagtt gaggcgcacc gccggcctcc ccctgcagcc
     4201 gcgtcgccgg atcagagcgg ggtcccgctc acccttcatc cggccggcga gcatcgacca
     4261 cgggccgggc ctgatcctat tccgcaacgc ctggcatacc gggctgccgt ggatatcgca
     4321 gtcggtcctg tcgggcgtgt gggggtcgtc gccctcgccc cggacgacga tcgcggcccc
     4381 ctgcgagggt tatcgcccgg tgcgctacc
//
LOCUS       DAOSEE010000163         4389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_7026_length_4389_cov_226.516151, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000163 DAOSEE010000000
VERSION     DAOSEE010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7026_length_4389_cov_226.516151"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="PLI76_09960"
     CDS             complement(<1..779)
                     /locus_tag="PLI76_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI62398.1"
                     /translation="MDLTRFRQETNVFEYGSRRVAKRATLTLLTAVLLALFLLFITLP
                     VASLFLRISPEAFLRSLAEPVVLDALSLSFITATVSTAIVVVFGTPLALVNARSDYAG
                     RELVDTLTDLPIVLPPAVAGLALLMAFGRRGVIGQYLDIFGIHIGFTTLAVVMAQVFV
                     ASPFYIRQARASFEAVDRLYEDAARTLGASRRTVALRITIPLAWGGLVSGAILSFARA
                     LGEFGATIMFAGNFQGRTQTMPLAIYTTMQGDMDASISLAI"
     gene            complement(748..1575)
                     /gene="modA"
                     /locus_tag="PLI76_09965"
     CDS             complement(748..1575)
                     /gene="modA"
                     /locus_tag="PLI76_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOI62399.1"
                     /translation="MRSSIPATLVAVSLLVIAGLLTAGCTTTEETPGPTTLTVFTAAS
                     LTGAFTDIGRAYEAQNADVKVDFVFDGSQTLRTQIEQGATPDIFVSASTKHMKALQDG
                     GFMDNSTVATFLGNSLALIVPVDNPAGITSLADLDEPGVKLVIGTKDVPFGDYTRQMF
                     EKMAADPAYGQAYQDAVMKNVVSEEMAVSSVVPKLALGEADAAIVYKSDVSKDDLTKV
                     TRIGIPAEYNVVATYPLGVLAESPSKAEAESFIAFVRGPDGSAVLTDYGFDPIPAGN"
     gene            complement(2165..2446)
                     /locus_tag="PLI76_09970"
     CDS             complement(2165..2446)
                     /locus_tag="PLI76_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62400.1"
                     /translation="MGLSDSVSSKQVGVRLPGHLYRWLKNKVDSGEYSNMAQSVIGEL
                     TKVKTLEEARCQETTSHGTYDDEPLSRMVNERIEGVRRELLDEVKRRRA"
     gene            complement(3033..4259)
                     /locus_tag="PLI76_09975"
     CDS             complement(3033..4259)
                     /locus_tag="PLI76_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOI62401.1"
                     /translation="MESGDLKIAWARQYMPVLSSIRERFIAEKPFAGMTIGMALHVEA
                     KTAVLVETLAAGGAEVYITGCNPLSTQDDVSAALNTRKGVHSYARRGADVEEYYAAID
                     RVLDARPAVTIDDGMDLIYRIHTERREVLDAVIGGCEETTTGIHRLRAMARDGALRFP
                     VIAVNDTPMKHFFDNVHGTGESSLASIMITTNVLIAGKHFVVAGYGYCGRGLAQKARS
                     LGARVIVTEVDPRRALQAHMDGFDVMTMDDAAPLGDIFVTTTGNAGIITGRHFPNLKD
                     GAILANAGHFNVEIDVEWLASHADSTVHRDGIDTYVLGGRAIHVLAEGRLVNLATPKG
                     MGHPIEVMDLSFAIQALSAEFIAKRGRDLAPGVHDVPAVIDEGVARLKLAALGLSIDR
                     LTPEQETYMSSWTIGT"
BASE COUNT          806 a         1324 c         1424 g          835 t
ORIGIN      
        1 atgattgcaa ggctgatcga ggcgtccatg tctccctgca tcgtcgtata gatcgcaagc
       61 ggcatcgtct gggtccggcc ctggaagttc ccggcaaaca tgatcgtggc gccgaactca
      121 ccgagcgccc gcgcaaacga gagtatcgcc ccggagacga gtccgcccca cgcaagcggg
      181 atcgtgatcc ggagggcgac ggtccggcgg gatgcgccga gggtccgcgc cgcatcctcg
      241 tagagccggt cgacggcctc gaagctcgcc cgcgcctgcc tgatgtagaa cggcgaggca
      301 acaaagacct gggccatgac caccgcgagc gtggtgaaac cgatatggat gccgaagatg
      361 tcgaggtact gcccgatcac gccccggcgg ccgaaagcca tcaagagcgc gagccccgcc
      421 accgccggcg ggaggacgat ggggagatcg gtgagggtat cgacgagttc cctccccgcg
      481 tagtcgctcc gggcgttcac cagggcaagc ggcgtcccga agacgacgac gatcgccgtg
      541 ctcaccgtcg cggtgatgaa ggagagggag agcgcatcga ggaccaccgg ttcggcgagc
      601 gagcggagga acgcttccgg cgatatgcgc aggaagagcg aggcaaccgg cagcgtgatg
      661 aagaggagga agagtgcaag aaggacggcg gtgaggaggg tgagggttgc cctcttcgct
      721 accctccggc tgccgtactc gaatacgtta gtttcctgcc ggaatcgggt caaatccata
      781 gtctgtcagt acggcgctgc cgtccgggcc ccggacgaat gctatgaacg attctgcttc
      841 ggccttgctc ggtgattccg caaggacccc gagcggatag gtggccacga cgttgtactc
      901 ggccggtatg ccgatccggg tcactttcgt caggtcgtct tttgagacat ccgacttgta
      961 gacgatcgcc gcatccgcct cgccgagcgc cagtttgggc actacggagc tcaccgccat
     1021 ctcctcggag acgacgttct tcatcacggc atcctggtag gcctgaccgt aggccggatc
     1081 ggcggccatc ttctcgaaca tctgccgggt atagtcgccg aagggaacgt ccttggtgcc
     1141 gatgacgagc ttcacgcccg gttcgtccag gtctgcaagg ctggtgatcc ctgccggatt
     1201 gtcgaccggg acgatcaggg cgagactgtt cccgaggaac gtcgccacgg tgctgttgtc
     1261 catgaacccg ccgtcctgga gggctttcat gtgcttcgtg ctcgcggaga cgaatatgtc
     1321 gggggtcgcc ccctgctcga tctgggtgcg gagggtctgt gaaccgtcga agacgaagtc
     1381 gaccttgacg tctgcgttct gtgcctcgta ggccctgccg atatccgtga aggcccctgt
     1441 gagcgatgcg gccgtgaaga cggtcagggt ggtcggcccg ggggtttcct cggtggttgt
     1501 acaccctgca gtcagaaggc cggcgatgac gagaagactt actgcaacaa gagttgccgg
     1561 gattgacgat ctcataataa caccattctc ataggggtta cggttccggg tatttatgtt
     1621 gtttactttg cccgtatatc aactaaaacg gacttacctt ccataccggt attaaaaggg
     1681 cacggtgcgg ccatggggaa ggatcccgcc ggagggagat cggtgccggg gcgagaggga
     1741 gttgtgaact acccctcctc tcgccggggg cacgacgccg taggcggcac catgctcttt
     1801 ccaggcgtgc gctgccgcct ctcctaacgt gtgggggagc ggaggcctct gtgcagcacc
     1861 ggcaacctgt gcggcccctc tcccccgtgt gtgggggaac ggaggcaccg gcgagcccgt
     1921 atacgctatc ggattcccct ctcccccgtg tgtgggggag cggaatgcaa tgggccggtt
     1981 gacggcggcc cgggaagcac ggtcccatgc ttgccatgag atgtcgcctt cggagcgtag
     2041 ccgtgatctc cggaagagag aaccgggcgg catccccatc gaaggcggga acgagcaccg
     2101 gacaggtccc gatcggcaag ggtgcatctc cttcgcccac gcggcggtta tgcacgggcc
     2161 cggatcaggc gcgccgccgc ttcacctcat ccaggagctc gcggcgaact ccctcgatgc
     2221 gctcgttcac catccgggag aggggttcgt catcgtaagt accgtgcgat gtggtctcct
     2281 gacatcgagc ctcttcgagc gtcttgacct tcgtcagctc cccgatgacg gattgggcca
     2341 tattcgagta ctcgccgcta tcgaccttat tcttcagcca ccggtagagg tgcccgggca
     2401 gtcgtacgcc cacctgcttg cttgagacgc tgtcggagag acccattatt tcattcagag
     2461 attccgtttg aacgattata ttacattgtt tttatttttc cggcgagata ccatgagata
     2521 cccataggta tcattaagcc gtcggtaacc caacaccgat gtatgagaca gagcgggttg
     2581 accgatttcg atctgccgaa aaccccggat gggaggttga aatgccaaaa gacaccccat
     2641 taaatgagat cgacttcgtg cgaagctgga ggagttgccg tatgcgcgga tcggatcgga
     2701 ggtcggggat cgcatgccca ccggatggca acggggccga tgcaagcgct ccctgtccct
     2761 tctgcgggta tcggcgccac gtcgaggatg ccatcgccgt cggtccggag gtacacaccc
     2821 ctcaccagtg cttccggtgc ggccgctggt tcgatggtaa aaggccggta tgccccctgt
     2881 gcgggtcacc gggagaactc ctcggagaag tcgttatttc cggggatggc ctgtatgtgc
     2941 agtaccgatg tcgcaggtgc gggaacggat ttatgcgcag gatcgacgat gacagcgcca
     3001 cccgccgtaa ataaaaaggg ggaagggcgg attcacgtcc cgatcgtcca gctgctcata
     3061 tatgtctcct gctcgggcgt caggcggtcg atcgagagac cgagcgcggc aagtttcagc
     3121 cgtgcaaccc cctcatcgat gacggccggc acatcgtgca caccgggggc aaggtctctg
     3181 ccgcgttttg ctatgaactc cgccgaaaga gcctggatcg cgaaactcag gtccatcacc
     3241 tcgatggggt ggcccatccc cttcggggtt gcgaggttca cgagcctccc ctcggcgagg
     3301 acgtggatgg ccctgccgcc gagcacgtag gtatcgatgc cgtcgcggtg aacggtggag
     3361 tccgcatgcg acgcgagcca ctcgacatcg atctcgacgt tgaagtgacc ggcgttcgcg
     3421 agtatggccc catccttcag gttcgggaag tgccggcccg tgatgatgcc tgcgttgccg
     3481 gtggtggtga cgaatatgtc gccgaggggt gccgcatcat ccatcgtcat gacgtcgaat
     3541 ccatccatgt gggcctggag cgccctccgg gggtcgacct cggtcacgat gacgcgggca
     3601 ccgaggctcc gcgccttctg tgcaagcccc cggccgcagt agccgtaccc ggcgacgacg
     3661 aagtgtttgc cggcgatgag gacgtttgtg gtgatcatga tcgacgcaag cgagctctcc
     3721 ccggtgccgt ggacgttgtc gaagaagtgc ttcatcggcg tatcgttgac ggcgatgacc
     3781 gggaaccgaa gcgccccgtc ccgggccatg gcgcggagcc ggtggatgcc ggtcgtggtc
     3841 tcctcgcacc cgccgatgac cgcatcgagc acctcccgcc gctcggtatg gatccggtag
     3901 atcaggtcca tgccgtcgtc gatggtgaca gcaggccggg cgtcgagcac ccggtcgatg
     3961 gccgcgtagt actcctcgac atcggccccg cgccgggcgt acgagtgaac ccccttacgg
     4021 gtgttgagcg ccgccgagac gtcgtcctgg gtcgaaaggg ggttgcaccc ggtgatgtac
     4081 acctctgcgc cgcctgccgc cagggtctcg accaggaccg cagtctttgc ctcgacgtgg
     4141 agggccatgc cgatggtcat gccggcaaaa ggtttctccg cgataaaccg ttcccgaatg
     4201 gacgagagta cgggcatgta ctgccgcgcc catgcaatct taagatctcc agactccatt
     4261 caatcaccag acccgttatc ccgcccggat gctcctccgg tttgcctgta aaggtatcct
     4321 cccggattac ttaaacctga ggggcggccg cagatggacc ggtgcggccc cgggaaggga
     4381 gcaggtcgg
//
LOCUS       DAOSEE010000164         4365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_7071_length_4365_cov_261.590255, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000164 DAOSEE010000000
VERSION     DAOSEE010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7071_length_4365_cov_261.590255"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..172
                     /locus_tag="PLI76_09980"
     CDS             2..172
                     /locus_tag="PLI76_09980"
                     /inference="COORDINATES: protein motif:HMM:NF022282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2551 domain-containing protein"
                     /protein_id="HOI62402.1"
                     /translation="MQEQFSISYHSVASMVGIIASRIGILHVRRNADGTNTVYELKDQ
                     YVDVVANILGTT"
     gene            224..493
                     /locus_tag="PLI76_09985"
     CDS             224..493
                     /locus_tag="PLI76_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62403.1"
                     /translation="MQSDTQEPGQSRGIYVTDDVRSYILQRGRDFRVCTSCGGPILLP
                     ITVKPPKATDLQVPVGDHTIYISKYQARYVDVIRRDMIPIFFEDF"
     gene            497..904
                     /locus_tag="PLI76_09990"
     CDS             497..904
                     /locus_tag="PLI76_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HOI62404.1"
                     /translation="MSFEELDHTADVLMRVRGATITEVFSDAGRAMFHVMYGSCEDRG
                     VKRRFALEAEDLESLLIDYLSELLFITDVENIVFCSFDIDIEGTRLSAVARGEPFDPA
                     RHAAGALIKGISYFGLEIVKEEETYAVDIIFDI"
     gene            914..2350
                     /locus_tag="PLI76_09995"
     CDS             914..2350
                     /locus_tag="PLI76_09995"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOI62405.1"
                     /translation="MLEGINKIGDLEWEVPIGYVADMRVPGRFFLSLALAETLEEGAV
                     RQLANVATLPGIVKHSLAMPDIHWGYGFPIGGVAAFDMTEGVISPGGVGFDINCGVRL
                     ITTPLTEADLAGKKRDLIERFFNAVPTGVGAKSTLRVSNKELSDVMVNGARWAVERGF
                     GIEEDLVRCEEEGAMPGADPGAVSAKARQRGLPQLGTLGAGNHFLEIQVVKEVVDPEA
                     ARAFGIAEGQICFMVHCGSRGLGHQVATDHLRILESALGKYRIRLPDRQLACAPVDSP
                     EGRAYYGAMVCAANYAWANRQVIMHEARKVFSGMFGIDYDDMRLIYDVAHNVAKFERH
                     DIDGASAEVCVHRKGATRAFGPGAPGIPREYAAVGQPVLIPGSMGTSSYLLRGTTTAM
                     QKTWGSTCHGAGRVLSRSKAKKEVRGRELREQLAGEGILVRAPHDAVLAEEAPEVYKP
                     SHEVVRVVHEAGLSGIVARLNPLGVIKG"
     gene            2359..2925
                     /locus_tag="PLI76_10000"
     CDS             2359..2925
                     /locus_tag="PLI76_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62406.1"
                     /translation="MGLLWDSPLMFSRLIEDCGACCEMVNPYMLASPFWRGRFTALIV
                     PTGFANPAYSNLLPALRASSGRIRRFIKSGGRLLAFGAGCPREDAYDWLPFPVTYAFG
                     YGPRAVRFTGESRYTSLFAGYDLAAVECDGTFPAHGGETLAVSASDEALLIEKTIGSG
                     TILVSSIHEYPSREFLKGFSCGDRETLF"
     gene            2945..3379
                     /locus_tag="PLI76_10005"
     CDS             2945..3379
                     /locus_tag="PLI76_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2111 domain-containing protein"
                     /protein_id="HOI62407.1"
                     /translation="MDMAMEQYIISASSTARDLAPIMICIHDLLGRLPVTARSRDHPG
                     IRIEDGKIIDQAYTGPILEEVLAGNTMKRVQPPAGPYRGIPVVVAPVRDSEGRAIGAI
                     GIVDVTGIFDLAGFMEQNAGIRKQVCGQDPCPLPTESPAAKK"
     gene            3385..4365
                     /locus_tag="PLI76_10010"
     CDS             3385..4365
                     /locus_tag="PLI76_10010"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOI62408.1"
                     /translation="MKDTAINVLKILEESPGPVSGERIAEQLGITRSAVWKQVRELRR
                     LGYGIASSRAEGYRLESATGKLLPYEIHKKLRTRSIGRQIRHFDRTASTSWIAKQLAA
                     ETDPEKLHGMVIIAEEQTGGVGRLGRAWVSPAGGIWITIVLKPKIPLDHLFMITMAGA
                     IAIARAIRKEYNISALIKWPNDIFAGDKKVGGLLLEIAAEADTIHYGLLGIGIDANIS
                     LGDLPPNLKDTVTSLQAEVGHEVDRVALLARVLREFELRYQQLEEGEYDSIIREWKSL
                     SLTLDQRVAIRTINKTFEGVAIDIDDHGALIIRKDNGRIERVIAGDCFQL"
BASE COUNT          912 a         1319 c         1328 g          806 t
ORIGIN      
        1 gttgcaggag cagttctcca tcagttacca ctccgtcgca tccatggtag gcatcatcgc
       61 gtcccggata ggcatcctgc atgtgagacg gaacgcagac ggaacaaaca ccgtttatga
      121 gttaaaagac cagtatgtgg atgtagtagc gaatatcctc gggactacgt aaaccccaaa
      181 acagactctt tttttacacc gaagaccaac ataaaagact atcatgcaga gcgatacaca
      241 ggagcctgga cagtcacggg gcatatacgt tacagacgat gtccggtcat acatacttca
      301 gagaggacgt gatttcaggg tctgcacatc ctgtggcggc ccgatcctcc tgccgataac
      361 ggtgaaaccg cccaaagcga ccgacctgca ggttcctgtc ggcgaccaca ccatctacat
      421 atcgaagtac caggcacgct acgtcgatgt catccgccgg gatatgatcc ctatattctt
      481 tgaagatttt taggccatga gttttgaaga actggaccat accgccgacg tcctcatgcg
      541 ggtacggggc gcaaccataa cggaggtctt ctccgatgca ggtcgggcca tgttccacgt
      601 gatgtacggc tcctgcgagg accggggtgt aaaacgacgg ttcgcccttg aggcggaaga
      661 tctcgaatct ctcctcatcg actacctctc ggaactgctc ttcatcaccg acgtcgagaa
      721 catcgtcttc tgctccttcg atatcgatat agaaggcacc cggctctctg ctgttgcaag
      781 gggcgaaccg ttcgaccccg cgcggcacgc cgcaggggcg ctcatcaaag ggatatctta
      841 cttcgggctc gaaatcgtta aagaagaaga gacctatgct gtagatatca tattcgatat
      901 atgagggatc gacatgcttg aaggaatcaa taagatcgga gacctcgagt gggaggtccc
      961 catcggatac gtcgccgaca tgcgggtgcc gggacgtttt ttcctctctt tggccctcgc
     1021 ggagacgctt gaggaagggg ccgtccgcca gctcgcgaac gtcgcaaccc tgcccgggat
     1081 tgtgaaacac tcgctcgcca tgcccgacat ccactgggga tacggatttc ccatcggtgg
     1141 tgtcgcagcg tttgacatga ccgaaggcgt catatctccc gggggagtcg gattcgacat
     1201 caactgcggc gtccgcctga tcacgacgcc cctcaccgag gccgacctcg ccggcaaaaa
     1261 acgagacctg atcgagagat tctttaacgc cgtgccgacg ggcgtgggtg caaagagcac
     1321 tctccgggtc tcaaacaagg agctctccga tgtcatggtg aacggtgcgc ggtgggcggt
     1381 cgagcgcggg ttcggcatcg aggaggatct cgtccggtgc gaggaagaag gcgcgatgcc
     1441 gggcgcggat ccgggtgcgg tcagcgccaa agcgcgccag agaggtttgc cgcagctcgg
     1501 gacgctcggc gccggcaacc acttcctcga gatccaggtg gtgaaggagg tcgtcgaccc
     1561 ggaggcggcg cgcgccttcg gtatcgccga aggccagatc tgtttcatgg tccactgcgg
     1621 ctcacggggt cttggccacc aggtcgccac cgatcatttg cggatactcg agagcgcgct
     1681 cggaaagtac cggatacgtc tccccgatcg gcagctcgcc tgcgccccgg tcgactcccc
     1741 tgaaggccgc gcttactacg gtgcgatggt ctgtgcagca aactacgcct gggccaaccg
     1801 gcaggtcatc atgcacgagg ccagaaaggt cttttcggga atgttcggga tcgactacga
     1861 cgatatgcgg ctcatctacg acgtcgcgca caacgttgcc aagttcgagc gccatgacat
     1921 cgacggtgcg tcggcggagg tctgcgtcca ccgtaaaggc gcgacacggg cattcgggcc
     1981 gggtgctccc ggcatccctc gcgagtacgc cgcggtcggg caaccggtcc tcatcccggg
     2041 gagcatgggg acgtcttcct acctcctccg cggcacgacg accgccatgc agaagacctg
     2101 ggggagcacc tgccacggcg cggggagagt gctcagccgg tcgaaggcaa agaaagaagt
     2161 cagggggaga gaactcaggg agcaactggc aggagaaggt atcctcgtgc gtgctcctca
     2221 cgacgccgtc ctcgcggagg aggcgcccga ggtgtacaaa ccgagccatg aggtggtgcg
     2281 cgtcgtacat gaggcgggcc tgtcgggtat cgtcgcccgg ctaaacccgc tcggggtgat
     2341 caaggggtga ccggcaggat ggggctgctc tgggatagcc ccctcatgtt ctcgcggctt
     2401 atcgaagatt gcggcgcctg ttgcgagatg gtcaacccct acatgctcgc ctcccccttc
     2461 tggcggggac gatttactgc gctcatcgta ccgaccgggt ttgcaaaccc ggcctactca
     2521 aacctcctcc cggcactccg ggcctccagt ggacgtatcc ggcgtttcat caagagcggg
     2581 gggcgattgc tcgccttcgg cgcggggtgc ccgcgggagg atgcctacga ctggcttccg
     2641 ttcccggtga cctacgcgtt cggctacggg ccgcgtgccg tccggttcac cggagagagc
     2701 aggtacacgt ccctcttcgc cgggtacgac cttgccgccg tcgagtgtga cgggacattc
     2761 ccggcccacg gcggcgagac ccttgccgtc tcagcgtccg acgaggcgct tctcatcgaa
     2821 aaaacgatcg gcagcggcac gatactggtt tccagcatcc atgaataccc atcgcgtgaa
     2881 ttcctcaaag gtttctcctg cggcgatagg gaaaccttat tctagctaca acatgatgaa
     2941 aagtatggat atggccatgg agcaatacat catctctgca tcctctactg cgcgcgatct
     3001 cgcccccatc atgatatgca tccatgatct gctcggccgt ctgcccgtca cggcgcggtc
     3061 ccgcgaccac ccgggaatca ggatcgagga tgggaagatc atcgaccagg cctacacggg
     3121 ccccatcctc gaggaagtcc tggctggaaa cacgatgaag cgggtccagc cccccgcagg
     3181 cccttaccgg ggcatcccgg tggtggtagc gccggtgaga gacagcgagg gcagagccat
     3241 cggtgcgatc ggcatcgtcg atgtgaccgg catcttcgat ctggccggct ttatggagca
     3301 gaacgcgggt atccggaagc aggtgtgcgg ccaggatccc tgcccgctcc cgactgagtc
     3361 tcctgcagcg aaaaagtgat gcgcatgaag gatacggcaa tcaacgtact gaagattctc
     3421 gaagaatctc ccggcccggt atcgggcgag cggatcgcag agcagctcgg aatcacccga
     3481 tcggccgtct ggaaacaggt gcgggaactc cgacggttgg gatacggcat cgcatcgtcc
     3541 cgcgcggagg ggtatcgcct tgagtcagcg accggcaaac tcctcccgta tgagatccac
     3601 aaaaaactcc gcacccggtc catcggaaga cagatccgcc acttcgatcg caccgcatcg
     3661 acgagctgga tcgcaaagca acttgccgcc gagaccgatc cggagaagct gcacggcatg
     3721 gtgatcatcg ccgaagagca gaccggcggg gtcggcagac tcgggcgcgc ctgggtctcg
     3781 cctgccggcg gcatatggat caccatcgtc ctgaaaccga agataccgct cgatcatctc
     3841 ttcatgatca ccatggccgg ggcgatcgca atcgcccgcg ccatcaggaa agagtacaac
     3901 atcagcgcac tcatcaagtg gcccaacgac atattcgccg gggataagaa ggtcggcggg
     3961 ctgctcctgg agattgccgc ggaggcggat accatccatt acggcctcct cggcataggg
     4021 atcgacgcca acatatcgct cggtgatctc ccgccgaacc tgaaggatac ggtgacctcg
     4081 ctccaggcgg aggtcggtca cgaggtcgac cgcgtggcgc ttcttgcccg ggtcctgcgg
     4141 gagttcgaac tgcgctacca gcagctcgag gagggcgagt acgactccat catccgtgag
     4201 tggaagagtc tctctctgac gctcgatcag cgggtggcca tcaggaccat caacaagacc
     4261 ttcgaagggg tggcgatcga tatcgacgac cacggcgccc tgatcatccg caaggataac
     4321 gggagaatag agcgggttat cgccggcgac tgcttccagc tctag
//
LOCUS       DAOSEE010000165         4295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_7226_length_4295_cov_267.922642, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000165 DAOSEE010000000
VERSION     DAOSEE010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7226_length_4295_cov_267.922642"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            387..1244
                     /locus_tag="PLI76_10015"
     CDS             387..1244
                     /locus_tag="PLI76_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOI62409.1"
                     /translation="MESVRYDINKYSPRQMVVLPLVLLLLAGIVLGYTMLATGLPLKP
                     GIDFAGGTAVTAFTSDSRETIEATFAGYPLLSVGEGIGGGKYIQFGPMDDARYQSLVA
                     LINEKYPDAKVDQIGETFGKTLQGQAFLALIFSFVGMSVVVLIAFRNLVPAGAVVLAA
                     FADIAVTAAIMQVVGIPLSLGTTAALLMLIGYSVDSDILLTTRLLKRKGKLDEKLAGA
                     FRTGIIMTTTTLAAIAAMWVVSTAGQVQIISDIASVLLIGLFVDLMNTWMLNAGILKG
                     YILKGGGKK"
     gene            1241..2701
                     /locus_tag="PLI76_10020"
     CDS             1241..2701
                     /locus_tag="PLI76_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071806 - protein transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecD"
                     /protein_id="HOI62410.1"
                     /translation="MKRETIVHLIKDWRVALLLLLVAGSLIGIYLTPPNPANGLEGNL
                     QFGLDLQGGSWLQMEFQSVVVAYSTDKPVGDLIENLQKSLEVDVVQIDQNHLEIRKSV
                     SRADLEPLFAASGAKIVTYHEGVSTFTADEVKRILNDKVNALGMQDARINLLTPTGSE
                     YPQYVRIELAGVDMATAQEIVGKQGMFEIRIQTSGNETEHVLFGDQITSVGVPQRDTR
                     SQAWGVGFTLSDAGADAFRDAALSHGAIDNPNAHNLVMLLDNKTVYSAPLSGDLAAQL
                     RSAPVRSLSASTGTGDAGLEAAMTLEIHLRAGALPVKVDVVGSGSVPAALGEHFKTTV
                     AIAGLFALLTVGVVIYYRYREPSIVIPMLAINFSEIIILLGIARFIQQLDLAAIAGLI
                     AVVGTGIDQLVIITDEVLHEGRVPSPSLYMKRYSRAFGIIAVSAATVMFAMLPLALMD
                     LSTLRGFAIITILGVLIGILVTRPAYGKIIMEILSK"
     gene            2762..3049
                     /locus_tag="PLI76_10025"
     CDS             2762..3049
                     /locus_tag="PLI76_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOI62411.1"
                     /translation="MGKPVLMDFFAEWCGPCHQQTPIIDELKKRMGDQVDIRKIDVGV
                     DSEQTRQYAAKYDIQFVPTLVIEKDGVLVRKLVGVQDLKSLESILKPLVGQ"
     gene            3046..3696
                     /gene="npdG"
                     /locus_tag="PLI76_10030"
     CDS             3046..3696
                     /gene="npdG"
                     /locus_tag="PLI76_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HOI62412.1"
                     /translation="MKIGIVGGTGDIGEGMALRLSSKYDVIVGSREEIKALATCETCR
                     ETLQVRGLKCSLAGVTNQHAVDEADIVVLAIPFKHVAPTLKTLTGFEGKIVVTPVNPI
                     ERTAYFYYAPPPEGSAAMMIKGMLPSSATVCAAFNNIAANKWQMLDEELDYSVAVCCD
                     DEGAKQTVMELVDSVSSLRAYDAGPLAAAAIVESVTPLLLNIARFNRMRDVGVKFV"
BASE COUNT          890 a         1284 c         1203 g          918 t
ORIGIN      
        1 gggtgatctc atatgtgctt gtgattcggt attttatata tatcttttct tttcatttat
       61 ttaatcggat attatgccag gaagacgacg attgtctgac agaatcggat gaaggtagat
      121 attatagccc cgttatccat aacaatccct gtaaatgccc tgatatttgt ttcagccaat
      181 atccgcccgg atggcctctc ccccccggcg cactcttccg gcccgccgca cataccggaa
      241 cgggcgatca ggggatcccc ggcccaccac gccgctcctc cccggggtgg tgccggggca
      301 atgcggcgtc acatgggaga aactcgattc ccgccgtata tccatgagca atcctttaac
      361 ctatcacaat cgtacactaa tacagcatgg aatctgtcag atatgacatc aacaaatact
      421 ctccgcggca gatggtagtg ctgcccctcg ttcttctcct cctcgccggg atcgtgcttg
      481 gctacaccat gctcgccacc ggcctgccct tgaaaccggg catcgacttt gcggggggaa
      541 cggcggtcac cgcattcacc tctgacagca gagagacaat agaagcgact tttgccgggt
      601 atccgctcct atcggtgggt gagggtatcg gcggcgggaa gtatatccag ttcggcccca
      661 tggacgacgc ccgataccag agccttgttg cgctcatcaa cgagaagtat cccgatgcga
      721 aggtggatca gataggggag acgtttggaa agacgctcca gggtcaggcc ttcctcgccc
      781 tgatcttctc gttcgtcggt atgtccgtcg tggttctcat agcgttccgg aacctcgtgc
      841 ccgcgggcgc ggtcgtcctc gcggcctttg ccgatatcgc cgtcaccgcc gcgatcatgc
      901 aggtcgtcgg catcccgctc tccctcggga cgacggcggc cctgttgatg ctgatcggct
      961 actccgtcga cagcgacatc ttgctgacca cgcgcctctt aaagcgcaaa ggaaaactcg
     1021 acgagaaact cgccggagcc ttcaggaccg gtatcatcat gacgaccacg acgcttgcgg
     1081 ccatcgccgc catgtgggtc gtctccacgg cagggcaggt ccagatcatc agtgatatcg
     1141 caagcgtcct cctcatcggt ctcttcgtcg acctgatgaa cacctggatg ctgaacgccg
     1201 ggatcctcaa gggatacatc ctcaaagggg gcggtaagaa atgaaacgcg agaccatcgt
     1261 acacctcatc aaagactggc gggttgccct gctccttctg ctggtcgccg gctccctcat
     1321 aggcatctac ctcacccccc cgaatccggc gaacgggctt gaggggaacc tccagttcgg
     1381 cctcgacctc cagggcggtt catggctgca gatggagttc caatccgttg tcgtagccta
     1441 ttcgaccgat aagccggtcg gggatctcat cgagaacctc cagaagagcc ttgaggtgga
     1501 tgtcgtccag atcgaccaga accatctcga gatcaggaag agcgtctcgc gcgccgatct
     1561 cgaaccgctc tttgcagcat cgggtgcgaa gatcgtcacg tatcacgagg gcgtatcgac
     1621 gttcaccgca gacgaagtga aacggatctt aaacgataag gtgaacgccc tcggcatgca
     1681 ggatgcgcgg atcaacctcc tcaccccgac gggaagcgag tacccgcagt acgtgcggat
     1741 agaactcgcc ggtgtggata tggcgacggc gcaggagatc gtcggtaaac agggcatgtt
     1801 cgagatccgg atccagacga gcgggaacga gaccgagcac gtcctctttg gcgaccagat
     1861 caccagcgtc ggtgtgccgc agagggatac caggagccag gcgtggggtg tcgggttcac
     1921 gctctccgat gctggcgcag atgccttccg ggatgccgcc ctcagtcacg gggcgatcga
     1981 caacccgaat gctcacaacc tggtgatgct ccttgataac aagacggtct acagcgcccc
     2041 gctctccggc gatcttgcgg cacagctccg gtcggccccg gtcaggtcgc tctccgcgag
     2101 caccgggacc ggcgatgcag ggcttgaggc tgcgatgacg cttgagatcc acctgcgcgc
     2161 cggcgccctg ccggtgaagg tggatgtcgt cgggtccggg tcggttccgg cggcactcgg
     2221 cgaacacttc aagacgacgg tcgccatcgc cgggctgttt gcgctcctca ccgtaggggt
     2281 cgtcatctac taccgctacc gcgagccttc gatcgtgatc ccgatgctcg ccatcaactt
     2341 ctccgagatc atcatcctgc tcggcatcgc acggttcatc cagcagctcg atctcgccgc
     2401 aatcgccggc ctgattgcgg tggtgggcac cggcatcgac cagctcgtca tcataacgga
     2461 cgaggttctc cacgaagggc gtgtcccgtc gccgagcctt tacatgaagc ggtacagccg
     2521 tgcgttcggt atcatcgccg tctcggcggc tacggtcatg ttcgccatgc tcccgctcgc
     2581 gttgatggat ctctccaccc tcagaggctt tgccatcatc accatactcg gcgtcctgat
     2641 cggtattctg gtcacgaggc cggcatacgg gaagataatc atggagatcc tctcaaagta
     2701 aatttttttc gaaaaccttt attgccccgt tcacacacaa tgtgtgaatg gggtatagtt
     2761 tatgggaaaa ccggttttga tggacttctt tgccgagtgg tgcggtccgt gccaccagca
     2821 gacaccgatc atcgatgagc tcaagaagag aatgggcgac caggtcgata tcagaaagat
     2881 cgacgtcggc gtcgattccg aacagacgcg acagtacgct gcgaaatatg atatccagtt
     2941 cgtacctacc ctcgtcatag agaaagatgg ggttctcgtc cggaaactgg tgggggtcca
     3001 ggatctcaag agcctcgaga gcatattaaa gccgctggtg gggcagtgaa gatcggcatc
     3061 gtcggtggga ccggtgatat cggagagggc atggcgcttc ggctctcttc gaagtacgat
     3121 gtgatcgtgg gatcacgcga ggagataaag gcgcttgcaa cctgcgagac ctgccgggag
     3181 accctgcagg tgcggggact gaagtgcagc cttgccggcg tcaccaacca gcatgcggtc
     3241 gatgaggcgg atatcgtcgt ccttgcgata ccgttcaagc acgtggcccc caccctgaag
     3301 accctgaccg ggtttgaggg taagatcgtc gtcacccccg tcaacccgat cgagcgcacg
     3361 gcctactttt actacgctcc tccccccgag ggctccgctg cgatgatgat caaagggatg
     3421 cttccttcga gcgcaaccgt ctgtgccgcg ttcaacaaca tcgccgcaaa caagtggcag
     3481 atgctcgacg aggaactgga ctactccgtc gccgtctgct gcgatgacga gggagcgaaa
     3541 cagaccgtga tggagctcgt cgacagcgta tcgagtcttc gtgcttacga tgcgggcccc
     3601 cttgcggctg cagcaatcgt cgaaagcgtc acgcccctcc tcctcaacat agcccggttc
     3661 aacaggatgc gggatgtcgg cgttaagttc gtgtaaaatt acgttatttt acaaaattca
     3721 tgcgggagag cacgggatcc tccttccagg ccggtcttcc ggtccgggga gatgccgctt
     3781 tctccatctc ctctcctctt catgcagaga tgcgagtgac cgccggcaga actcggtgtc
     3841 tgctgtttta catccgcctc gcacgaggtt gtgccggcgc gccatgcgtt cccggcgtga
     3901 aaggccacag agatgttagt gagtacattc gcctgagcgg agtcaagacc ttttcccatt
     3961 tactttttgg atgtcatact gtcgcagtaa aagccattga ttgagggtta tggcgagaat
     4021 ggtgagaaac atgatcaaag ggacaatgaa ccattgctgt tctggataat acaggctgcg
     4081 ccagtattca accccctcag cgataacgac gataattgct gttcctattg tcattctcgc
     4141 taacaaaccc accatacgac caccacatca actcgatctt tccgtacgtt attaatcctt
     4201 gcacgagtcg tcccccccat ggcgatagcc accacgatcc ggtgcacggc tgctcaccgg
     4261 ctccaccccg gatacggctg ctctcctgat atacg
//
LOCUS       DAOSEE010000166         4283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_7257_length_4283_cov_227.450095, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000166 DAOSEE010000000
VERSION     DAOSEE010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7257_length_4283_cov_227.450095"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="PLI76_10035"
     CDS             complement(<1..926)
                     /locus_tag="PLI76_10035"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOI62413.1"
                     /translation="MTIRDPGRGFHCRVALRISSNGRNAYRLTRIHVNGEEFAVRTMR
                     QREVEIAVDWAAQEGWNPGVHDADAFYAADPEGFFIAELDGEPIGSLSVVNYSETFAF
                     AGLYILRPEYRNRGYGSRLFAAGMAHAGGRNVGGDGVLSMQEKYRTRSGFTLVYRNIR
                     FEGAGGGNEPEGPVDIVAVPFERLAAYDARHFPAPRLRFLASWIRQEGTCGRAVIGRD
                     GEVAGYGVIRRCRHGCKIGPLFADTPEVAEDIFLALSAQAPDEPVYLDVPEPNAAAVA
                     LARRHGMAPVFETARIYTKAAPDLPLDEIFGVT"
     gene            1185..2486
                     /locus_tag="PLI76_10040"
     CDS             1185..2486
                     /locus_tag="PLI76_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HOI62414.1"
                     /translation="MKEDLGQIIDRSFRPATEILLDLLDDGFKCAFSISGVVVEHLER
                     WYPEMLELISRAATHRNAEILAETYYHSVAGQFADLAEFTDELLMHRDLMKEQFATVP
                     TTFVHTDYPITPATADALAGAGIEAAIIEGGAPPAPERDPNHTYTYHGLPVLVTHCDL
                     TDDIAVRFPSREWDRWPLMADRYAKWLAVSPGDCITLFLDYRIFGDVIGPETGILDFL
                     RALPAALAGEGIMTLHPSEAVRTIPPQADIEETGGPQRTILQQSALEALEEAGDLVTD
                     RAVWRCLLETDHIRRMAMRSPSCGRSLHAVSHQATYDYFTSFMQILSHAEERSASRTR
                     SPKAAFSLRCVPPDKAFSFSSYDRPAGYAAYSLQELASMLDFTPDDVIRYHVERDDIY
                     RWIDQVVGDGKLAKKVQGISDRNELRSTIQKRIDELWKRLR"
     gene            2468..3619
                     /locus_tag="PLI76_10045"
     CDS             2468..3619
                     /locus_tag="PLI76_10045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOI62415.1"
                     /translation="METLKIAFFCWESLHSPFKVGGLAPAATHLAEHLARRGHEVHFF
                     TRGEPGDAEVNGIHYHYCRPYGDNIIEYCKTMSNMLVDAFRACDTPAFDILHFHDWHL
                     VEALHTLRDRNTVLSYHSTEYGRHGGNFGGWWEFKEISGKEWYGGYIAKTVTTVSNST
                     KTEVMWLYNVPEWKVTVIPNGIDPDAYYARVDPGEVKKRYGIHPLAPVVLFVGRLTYQ
                     KGPDLLMQAIPRILATRWDVQFLFAGTGDMRGYLEGMAHGLPVQFLGYIADREHVQLL
                     NACDLIAIPSRNEPFGLVLTEAWSAERCVVATDVGGLSENIDNYINGIKVPVRPDSIA
                     WGICHLIDDPVYMQSLGKAGRRKVMEKFRWAVITERMLSVYRKVLSDSP"
     gene            3635..>4283
                     /locus_tag="PLI76_10050"
     CDS             3635..>4283
                     /locus_tag="PLI76_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HOI62416.1"
                     /translation="MQQALPDITRTRSLITDYDVYLFRQGSHSRLYDRLGSHPAVIDD
                     RRGTFFAVWAPNAVEVSVIGDFNGWKAGENPLSLRNDDSGIWEGFLPDIGQGTLYKYH
                     IRSRYNNYSVEKGDPFARFWEIPPKTASIIWDIAYTWRDRAWMRGREEENSPDAPISI
                     YEVHAGSWRKVPEEENRSLSYREAADELAEYLVDAGFTHVEFLPIMEHPFYASWGY"
BASE COUNT          877 a         1414 c         1224 g          768 t
ORIGIN      
        1 gtcaccccga agatctcatc gaggggcaga tcgggggctg ccttcgtgta tattcgcgcc
       61 gtctcaaaga cgggagccat cccgtgccgg cgggcgagcg ccacggcggc ggcgttcggc
      121 tccggcacat caaggtagac cggttcatcc ggcgcctgtg ccgagagcgc gaggaagata
      181 tcctccgcca cctccggcgt atctgcaaag agcggtccga tcttgcagcc gtgacggcac
      241 cttcgtatga cgccgtaccc cgcgacctca ccgtcccggc cgatcaccgc cctgccgcac
      301 gtcccctcct gccggatcca ggatgctagg aatcggaggc gcggcgccgg gaaatgccgg
      361 gcatcgtagg cggcgagccg ctcaaacggc accgcaacga tgtcgacagg accttcgggc
      421 tcgttgccgc cgccggcacc ctcgaacctg atgttcctgt agacgagcgt gaacccggac
      481 cgcgtacggt acttctcctg catcgaaagg acaccgtcgc ccccgacgtt ccggcccccg
      541 gcgtgggcca tgccggcggc aaacagcctg ctcccgtagc ccctgttccg gtattcgggc
      601 ctcagtatat acaggccggc aaaggcaaac gtctcgctgt agttgacgac cgagagcgat
      661 cctatcggct cgccgtcgag ctcggcgatg aagaacccct ccggatcggc tgcataaaag
      721 gcatccgcat cgtgaacccc cggattccac ccctcctgtg ccgcccaatc gacggcaatc
      781 tcgacctccc gctgccgcat cgtcctcacg gcaaactcct ctccgttcac gtgaatcctc
      841 gttaagcggt aagcgttcct cccatttgag gatatccgga gagcaacacg gcagtgaaac
      901 cctctcccgg gatcccggat ggtcatgatc ccaccgggag gtaggatcta ccctttattc
      961 gggcatcagg accctgtgga gggcaaaaga ccaaaagatc ttccgggttg atgaccttta
     1021 tatacggaaa cagcgaccta cctcgtatga gaggcgaggg cgcaaatccg ccggcggtct
     1081 gtctggggtt cgacatccac tatccctgtc atctcaatcc cggatttcga ccagatatgg
     1141 taaagggaaa gagaaaccca aaggacagct actttaatcc ggatatgaag gaagatctgg
     1201 ggcagatcat cgaccggtcc ttccgaccgg cgacggagat cctcctcgat ctcctcgatg
     1261 acgggtttaa gtgcgctttc agtatatccg gggtggtggt ggaacacctc gaacggtggt
     1321 atccggagat gctcgaactc atatcccgcg cggcaaccca ccggaacgcc gagatcctcg
     1381 cagagaccta ctaccacagc gttgccgggc agttcgccga tctggcggag ttcacggacg
     1441 agcttctcat gcaccgcgac ctgatgaagg agcagttcgc aaccgtcccg accacgtttg
     1501 tccataccga ttatcccatc accccggcca ccgcagatgc gctcgccggt gccggtatcg
     1561 aggcggctat catcgagggg ggcgcgcccc ccgcccccga gagagaccct aaccacacct
     1621 acacctacca cggcctcccg gtcctcgtca cccactgcga cctcaccgac gacatcgccg
     1681 tgcggtttcc ctcaagggag tgggataggt ggccgctgat ggccgaccgg tatgcaaaat
     1741 ggcttgccgt atcccccggc gactgcatca cgctcttcct cgactaccgc atattcggag
     1801 acgtcatcgg tcccgagacg ggtatcctcg atttcctgcg cgcactcccg gcagccctgg
     1861 ccggggaggg gataatgacc ctgcacccat cagaagctgt gcggaccatc cccccgcaag
     1921 cggatatcga agagaccggg ggtccgcaaa ggaccatcct gcagcagtcc gcactcgagg
     1981 cacttgagga agccggcgat ctggtgactg accgggcagt ctggcgctgc ctccttgaga
     2041 cagaccatat ccggaggatg gccatgcgct cgccctcgtg cggaagatcc ctccacgccg
     2101 tcagccacca ggcaacctac gactacttca cctcgtttat gcagatcctc tcccacgccg
     2161 aagagcggtc tgcgtcccgc acacggtcac cgaaggccgc cttctcgctc cggtgcgtcc
     2221 cccccgataa ggcgttctcc ttctcatcgt acgacagacc ggccgggtat gccgcctaca
     2281 gcctgcagga acttgccagc atgctcgatt ttactccaga cgacgtcatc cggtaccatg
     2341 tagagcggga tgacatttac cgctggatcg atcaggtcgt cggcgatggg aaactggcga
     2401 agaaagtcca gggcatcagc gatcgcaatg aactccgctc aacaattcaa aagaggatcg
     2461 acgagttatg gaaacgctta agatagcatt cttctgctgg gagtcactcc actccccatt
     2521 caaggtgggc gggctcgcac cggcggcaac ccaccttgcc gaacatcttg cccgaagagg
     2581 gcacgaggtc cacttcttca cccggggaga acccggggat gcagaggtca acggcatcca
     2641 ctaccattat tgccgccctt acggcgacaa catcatcgag tactgcaaga ccatgagcaa
     2701 catgctcgtg gatgcgttcc gcgcctgcga caccccggcg ttcgatatcc tccactttca
     2761 cgactggcat cttgtcgagg cgctgcatac cctccgggac cgaaacaccg tcctctctta
     2821 ccactccacc gagtacgggc ggcacggcgg gaacttcggc ggttggtggg agttcaaaga
     2881 gatatcgggc aaggagtggt acggcggcta cattgcaaag acggtcacga ccgtctcgaa
     2941 ctcgacgaag accgaggtga tgtggctcta caacgtcccc gagtggaaag tcaccgtcat
     3001 cccgaacgga atcgatcccg atgcctatta cgcccgggtc gaccctggcg aggtgaagaa
     3061 acgctacggc atccacccgc tcgcacccgt cgtcctcttc gtcgggcgcc tcacctacca
     3121 gaaggggccc gacctcctga tgcaggccat accccggatc ctcgccacac gctgggatgt
     3181 gcagttcctc ttcgccggca ccggagatat gcgcggctac ctcgagggga tggcccacgg
     3241 ccttcccgtg cagttcctcg gctacatcgc cgatagagag cacgtccagc tcctgaacgc
     3301 ctgcgacctc atcgccatac cgagcaggaa cgaaccgttc gggctcgtcc tcaccgaggc
     3361 ctggagcgcg gagcgttgcg tcgtcgcgac cgatgtcggg gggctctccg agaacatcga
     3421 caactacata aacgggatca aggtccctgt ccgaccggac tcgatcgcct gggggatctg
     3481 ccacctcatc gacgacccgg tctacatgca gagcctcgga aaagccggcc gccggaaagt
     3541 gatggagaag ttccggtggg ccgtcatcac ggagaggatg ctatccgtct acagaaaggt
     3601 tctctccgac agtccctgat cccggaggga gaccatgcag caggcgctac ctgacataac
     3661 gaggacccgg agtctcatca ccgactacga cgtctacctc ttccggcagg gaagccattc
     3721 ccgcctgtac gatcgcctgg gatcgcaccc tgccgtcatc gacgacagac ggggaacctt
     3781 ctttgcggtc tgggcgccga acgcggtgga ggtctcggtc atcggagact tcaacgggtg
     3841 gaaggcgggc gagaatccgc tctctctccg gaacgacgat tccggtatct gggaagggtt
     3901 tctgccggat atcggccagg gaacgctcta caagtaccat atccggagca ggtacaacaa
     3961 ctactccgtg gagaaaggcg atccgttcgc ccgcttctgg gagatcccgc caaagactgc
     4021 atccatcatc tgggatatcg cctatacatg gcgcgatcgg gcctggatgc ggggccgcga
     4081 ggaggagaac tcccccgatg ccccgatatc catatacgag gtgcatgcag gctcatggcg
     4141 gaaggtgccg gaggaggaga accgttccct cagttaccgg gaggcggcag acgaactcgc
     4201 cgaatacctg gttgatgccg ggttcaccca cgtcgagttc ctccccatca tggagcaccc
     4261 gttctacgcc tcctggggct acc
//
LOCUS       DAOSEE010000167         4245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_7350_length_4245_cov_284.727685, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000167 DAOSEE010000000
VERSION     DAOSEE010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7350_length_4245_cov_284.727685"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="PLI76_10055"
     CDS             complement(<1..643)
                     /locus_tag="PLI76_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844289.1"
                     /note="GTPase; similar structure to tubulin; forms
                     ring-shaped polymers at the site of cell division; other
                     proteins such as FtsA, ZipA, and ZapA, interact with and
                     regulate FtsZ function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOI62417.1"
                     /translation="MQTIINEALKHAEREKYLKTDSGEAEDDLLGQPRIVIVGCGGAG
                     NNTVNRLYHMQVSGAETIAINTDKQHLDMIQADKRVLVGKSLTKGLGAGGFPDVGRRA
                     AEMARPTLEGLLCDADLVFITAGMGGGTGTGTAPVVAQIAKEQGAIVVGMVSYPFQVE
                     KARLLRAEEGLEQLSTTADSVIVLDNNRLIKFVPNLPLGQAFSVMDQLIAETVK"
     gene            complement(657..833)
                     /locus_tag="PLI76_10060"
     CDS             complement(657..833)
                     /locus_tag="PLI76_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HOI62418.1"
                     /translation="MDRITIRLPRQQVEMLERLVEAGEFPTVSEAVRYSVRALLERHG
                     NRALREADQISFQV"
     gene            1034..2032
                     /locus_tag="PLI76_10065"
     CDS             1034..2032
                     /locus_tag="PLI76_10065"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOI62419.1"
                     /translation="MRENLPERMEHGGRVRWHCMREGGELIDFSANINPYPPIIPWTP
                     DPSALKDYPDDRYEALKEVIGSTFGRDAAEITVGNGSVELIRAFCSAVLGEGDTACIQ
                     PPTFAEYEMAARLAGARCTADESGAAVRFLCNPNNPTGRLSTRPEILGALDETANEGG
                     YLFVDEAFIELADPRQSVVDVSHEHLFTLRSLTKSFTVPGIRFGYGFGTPELIEQVET
                     MRLPWTVNAFAEAFAIEAFRHYDLLERSRERIAREREWLCGRLTDLGLAYEPPSANYV
                     LMEVPLEAGVLVARLLSHGILARDCRSFGLPRHIRVAVRTREENRQLIEAFEACLP"
     gene            2020..2625
                     /locus_tag="PLI76_10070"
     CDS             2020..2625
                     /locus_tag="PLI76_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOI62420.1"
                     /translation="MLALIMAGGQGSRLRMGEKPLVTICGKPMLAYVIDAFASAGHEV
                     VVVASHRTPFTKNWCRAQGIVLYEAEGLGYVEDICAAADDLGEAGTPFFTCVSDLPCL
                     APDIIAGVEEAWRRAKTPACSTWVPRSLCEEHGCRTQYTETVDGVPACPAGINILTAG
                     GFDGLEEELCLLLHDRRLAFNVNTREELALVQGYLCQKRVP"
     gene            2751..3980
                     /locus_tag="PLI76_10075"
     CDS             2751..3980
                     /locus_tag="PLI76_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HOI62421.1"
                     /translation="MESCREIELEGHIIDSGIMTLVFDRIMDMGGEFEILTFNVGKQK
                     TDTSYARLRVTAPTGRQLDAILSELHRLGARTPEISDVTLVPAEGDRILPKGFYSTTN
                     HPTFVKYRDEWLPVEHIEMDCHIVIDEAEKRAFCKPIAKIKAGDAVVVSEAGVRVVYP
                     ERPRKVSTFEFMHGTVSSERPSEAIIAKIACEILEMKKAGEKIALVGGPAIVHTGAAD
                     ALAKMIHEGYIDVLFAGNALATHDIESNLFGTSLGMNVRTGTLVTGGHKHHIYAISEI
                     MRAGSIKNAVDQGVVTGGIMYECVKKGIPFVLAGSIRDDGPLPDVITDVVEAQDTMRR
                     HIHDVGMVLMVGTLLHSIAVGNCLPSYVKTVCVDINPASVTKLMDRGTTQAIGVVSDA
                     GTFLPLLLEQLEEQETC"
BASE COUNT          816 a         1303 c         1303 g          823 t
ORIGIN      
        1 ccttgacggt ctccgcgatg agctggtcca tgaccgagaa cgcctggccg agcggcaggt
       61 tcggcacgaa cttgatgaga cggttgttgt cgaggacgat gaccgagtcg gcagtggtcg
      121 agagctgctc aagcccctct tccgcgcgga ggagtcgggc tttctcgacc tggaagggat
      181 agctgaccat gccgacgacg attgccccct gctcttttgc tatctgcgca acgaccggcg
      241 ccgtccccgt accggtcccg ccgcccatgc cggccgtgat gaagacaagg tccgcatcac
      301 agagcaggcc ttcaagggtc ggccgggcca tctcggctgc gcgcctgccg acgtccggga
      361 acccgccggc cccaaggcct ttggtgagcg attttccgac aagcaccctc ttgtcggcct
      421 ggatcatgtc caggtgctgt ttgtccgtgt tgatcgcgat cgtctctgca ccactgacct
      481 gcatgtggta aaggcggttg acggtattgt tgcctgcccc gccgcatcct acgatgacaa
      541 tccgtggctg gccgaggaga tcgtcctctg cctcgcctga gtccgtcttc aggtattttt
      601 ctcgttctgc atgttttaat gcctcgttga tgatcgtctg catctgacaa tacctcctaa
      661 acctggaatg aaatttgatc ggcctcgcgt aaggcacggt tgccatgcct ttcgagaagc
      721 gccctgaccg aataccgcac agcctccgat actgtgggga attcgccggc ctccaccaac
      781 ctctcgagca tttcaacctg ctgccggggc aatctgatag ttatccgatc catcatctca
      841 atcactcata tctgacatat ggcagctcta cgtctgtcat ttgtcagaca atcgtaatct
      901 tcttgtctga ctgtatcaga tatggcataa taacatcggt atgatataaa taccttctga
      961 aatgagtcca gacacgtcac aacggtttta tggatagcag cagacaacgt accttcacag
     1021 gattgccacg atcatgagag aaaacctccc tgaaaggatg gaacacggcg gccgggtacg
     1081 ctggcactgc atgcgagagg gtggcgagct gatcgatttc agtgctaaca taaatccata
     1141 tccccctatc atcccctgga caccggaccc ttcggcactg aaagactacc ccgacgaccg
     1201 ttatgaggcg ctcaaagagg tgatcggcag cacgttcggg cgcgatgcgg ctgagatcac
     1261 cgtgggcaac ggatctgtcg aacttatccg cgcgttctgc tccgccgtgc tcggcgaggg
     1321 ggataccgcc tgcatccagc cgccgacatt tgccgagtac gagatggcgg cgaggctcgc
     1381 cggcgcccgg tgtacggccg atgagagcgg tgccgccgtc cggttcctct gtaacccgaa
     1441 caacccgaca ggcaggctct ctacccgccc cgagatcctc ggggcgcttg atgagacggc
     1501 gaatgaggga gggtatctct tcgtcgatga ggcgttcatc gaactcgccg acccgcgcca
     1561 gagcgtcgtc gacgtctccc acgagcacct ctttaccttg cgctccctga cgaagagctt
     1621 caccgtgccg ggcatccggt tcggatacgg gttcggcacg cccgaactca tcgagcaggt
     1681 ggagaccatg cgcctcccct ggacggtgaa tgcgtttgcc gaggccttcg ccatcgaggc
     1741 gttccggcac tacgatctgc tcgaacgttc gcgggagcgg attgcccggg aacgtgaatg
     1801 gctctgcggc cgccttacgg acctcggtct cgcatacgaa cccccatctg cgaactacgt
     1861 cctgatggag gtcccgctgg aggcaggggt gcttgtcgcg cggctcctct cccacggcat
     1921 cctcgccagg gattgccggt cgttcgggct accccgccac atccgtgtag cggtgcggac
     1981 ccgcgaggag aaccggcaac tcatcgaggc attcgaagca tgcttgccct gatcatggcc
     2041 ggggggcagg gctcccgtct ccggatgggc gagaaaccgc tcgtcaccat ctgcggaaaa
     2101 cccatgctcg cctacgtcat cgacgccttc gcatccgcag ggcacgaggt ggtcgtggtc
     2161 gcctcgcacc ggacgccgtt taccaaaaac tggtgccggg cacaggggat tgtcctgtat
     2221 gaggccgaag ggctcggcta cgtcgaggat atctgtgcgg cggcggatga cctcggagaa
     2281 gcaggaacgc ccttcttcac ctgcgtctcc gacctgccct gtcttgcccc cgatatcatc
     2341 gccggcgtcg aggaggcctg gcgccgggca aaaacgccgg catgctccac gtgggtgccg
     2401 cgcagtctct gcgaagagca tggctgccgc acgcaatata cggagacggt cgacggcgtg
     2461 ccggcctgcc cggcagggat caatatactg acggcgggcg gctttgatgg ccttgaggag
     2521 gagctctgcc ttctcctgca cgatcgccgg ctcgccttca acgtcaacac gcgcgaggag
     2581 ctggcgctgg tgcaggggta cctctgccag aagcgggtcc cctaacctcc ggcggacggc
     2641 cgcacagaaa agcctccggt cttcccgaac ttcgctccac gggagcatct ctcccgaaca
     2701 atcgtttttt gggagaaact ctattatcgg accatcgctg atctctttat atggagtcat
     2761 gccgggagat cgaactcgag gggcatatca tcgattcggg cattatgacc ctcgtgtttg
     2821 acaggatcat ggatatgggt ggggagtttg agatcctcac gttcaacgtc gggaagcaga
     2881 agacggatac gagttacgcg cggctgcgcg tgacggcacc caccgggcgg caactcgacg
     2941 ccatcctctc cgagctgcac cggctcggcg cacgcacccc cgagatcagc gacgtcaccc
     3001 ttgtaccggc agagggcgac cggatcctcc cgaaggggtt ctactcgacc accaatcatc
     3061 cgacgttcgt gaagtaccgg gatgaatggc tgccggtcga gcatatcgag atggactgcc
     3121 acatcgtgat cgatgaggcg gagaagcggg ccttttgcaa accgatagcg aagataaagg
     3181 ccggcgatgc cgtcgtcgtg agcgaggccg gggtgcgcgt ggtctacccc gagcggccgc
     3241 ggaaggtcag cacgtttgag ttcatgcacg ggaccgtctc gtcggagcgg ccgagcgagg
     3301 cgatcatcgc aaagatcgcc tgcgagatcc ttgagatgaa gaaggccggg gagaagatag
     3361 ccctcgtcgg cggcccggcc atcgtccaca cgggagcggc cgacgcgctt gcgaagatga
     3421 tccacgaagg ctacatcgac gttctcttcg cggggaacgc gcttgccact cacgatatcg
     3481 agtccaacct cttcggcacg tcgctcggga tgaatgtaag gacaggtacg ctcgtcaccg
     3541 gggggcacaa gcaccacatc tacgccatca gcgagatcat gcgtgccggg tctatcaaga
     3601 acgcggttga ccagggggtc gtcaccgggg ggatcatgta cgagtgcgta aagaagggca
     3661 ttccgttcgt gcttgccggc tccatacggg acgacggacc gctccctgac gtaattacgg
     3721 atgtcgtgga agcgcaggac accatgcgtc gtcacatcca cgacgtcggg atggtgctga
     3781 tggtcgggac cctccttcac tccatcgccg tcggaaactg cctcccctca tacgtcaaga
     3841 cggtctgcgt cgatatcaac cccgcatcgg tgacgaaact gatggaccgg ggcacgacgc
     3901 aggcgatcgg cgtcgtgagc gatgccggga cgttcctccc gctgctcctc gagcagctgg
     3961 aggagcagga gacgtgttag cgggcaatct cggggtgggg gtgccggcag cccccgcatg
     4021 ctcttttgtg tgagccgggg gcgcgtggat gccggtgaag aaccggggcc gggcttgtct
     4081 ctgccggcaa atcggcgcca acattgctcc tccaatatga cttcccacca tatatacgcc
     4141 cgggtattgt ccccaggcag gggcggagcg ggggacgcca agacatacgg tggttggagt
     4201 gtgggagaca ggggaggggg aacaagttcc ccctccccgt cagcc
//
LOCUS       DAOSEE010000168         4209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_7424_length_4209_cov_223.374338, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000168 DAOSEE010000000
VERSION     DAOSEE010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7424_length_4209_cov_223.374338"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="PLI76_10080"
     CDS             <1..1067
                     /locus_tag="PLI76_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HOI62422.1"
                     /translation="GGAAEVNLYDRDIANIRLARALRLPIVLVADIERGGVFAQVYGT
                     LALLPEDIRPLVAGVIVNKFRGDPWLFASGVAKLEELTGVPVLGVVPYTDIPLPSEDS
                     LSLADKGRQAAGGAIRIAVVRLPRIANFTDFELLERHAAVDYVPPGTPLSGYDCIVLP
                     GTKNTVEDLAALSCHGTGEELRRARERGVPVIGICGGYQMLGSRIVDAGTESGTPGEH
                     PGFGLLDVTTTFSGYHKTTVQVRRRATGPGPILSRMGEVAGYEIHMGETERGSTLEAF
                     AGEGAVTPDGLVFGTYMHGLFQNPGAVNALLAYLSERRGVPFEPATAGGDAAYDALAR
                     HFEEHVDMDAIVAWFGDGEE"
     gene            complement(1192..1515)
                     /locus_tag="PLI76_10085"
     CDS             complement(1192..1515)
                     /locus_tag="PLI76_10085"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOI62423.1"
                     /translation="MKVRAIITGRGVHDVGYRLYLLERALDTGFQRFYAKIQVQNGAE
                     QVVVQYEGEPGQVDALGSIIREEHPPGVDIEHIAFEACAWDVVPVTDYLDLLIVEQLS
                     LIAPL"
     gene            complement(1649..2683)
                     /locus_tag="PLI76_10090"
     CDS             complement(1649..2683)
                     /locus_tag="PLI76_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOI62424.1"
                     /translation="MAFKEIIDDFLNRNQPRGPAAEESFADEPFGEREIFSRIENRRK
                     YQEGFYRFIRHPLRVMIEDPVTVLFVSVPVALFLLIGGSASLVMSYGPGILLTTTMVD
                     DIFVFALLIAIVPLAALDLKEALRVSSIEASLPNFFRDVAGMNDSGMTLPHAIHIVSE
                     GEYGALTPHIRKLDTEMSWGVPFVEAIRRFGKVVNTPLAERSVDLIAHASSAGGDVSE
                     VLRAAAHDAYEFFNLKSERRNGMMIYMIIIVMSFFVFLFVIGVLSSTFLTTMAEAGEA
                     VAKSGSSQMFMGGVDLFLYNRLFSHSALIQGFFSGLAAGVMGEGRVLAGLKYSAIMVL
                     IAWIAFRFFI"
     gene            complement(2685..3608)
                     /locus_tag="PLI76_10095"
     CDS             complement(2685..3608)
                     /locus_tag="PLI76_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOI62425.1"
                     /translation="MNGYERFCFNLLGRDLKARRGEYIDLRNDLTGARMNTPFEAYLA
                     TAYVSSIAVGLVAAALIGILTYALRVPEMISYHGAVPEVFYALNAYKLLIGTVVITLL
                     SLLIFGGITYLIFLLYPGIRAGERRRNIDATLPYAINYVTAMSSAGITPDEVFRLLGQ
                     SRIYGESAVEARYVARETDFFGKDLLDALRSVSQATPSERMREFLQGAVASISSGSNL
                     TEYFRAKARQYTLENNQQQKTFLETLGLIAESYVTAMVAGMLFLIILQSVMTILSGDA
                     NPFFLYIIIYLIVPFGSIMFVILISSMTPEV"
     gene            complement(3605..>4209)
                     /locus_tag="PLI76_10100"
     CDS             complement(3605..>4209)
                     /locus_tag="PLI76_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOI62426.1"
                     /translation="EIDMYELLRAALRQRPEYILVGEVRGREALTLFQAMSTGHVTYA
                     TMHADSVASAVHRLENPPIDVPRNMLSALSLVSIQVQARVGGQRIRRNKQLIEILDID
                     PRTNELITNEVFRWHPATDEIRYSGKSYILEQIMEDRGWSEERMREELKRRQEVLEWM
                     RIKKIRHFRDVSKILVSYFRDPESVIERVRTDLYGEGSPA"
BASE COUNT          787 a         1340 c         1352 g          730 t
ORIGIN      
        1 ccgggggagc ggcggaggtg aacctctacg accgcgacat cgcaaacata aggcttgccc
       61 gcgccctccg cctcccgatc gtcctcgtcg ccgacatcga acggggcggt gtctttgcgc
      121 aggtctacgg gacgctcgcc ctcctcccgg aggacatccg gccgctcgtc gccggggtca
      181 tcgtcaacaa gttcagagga gacccctggc tctttgcatc gggggtcgcg aaactcgagg
      241 agctcacggg tgtcccggtg ctcggcgtgg tgccgtacac ggatatcccc ctcccgagcg
      301 aggactcgct ctctctcgcc gataagggcc ggcaggcggc cggaggggcg atacggatcg
      361 ccgtcgtccg tcttccccgg atagcgaact tcaccgactt cgagctcctc gaacgccacg
      421 cggcggtgga ctacgtcccg cccgggacgc cgctctccgg ctacgactgc atcgtcctcc
      481 cggggacgaa gaacaccgtc gaggacctcg ccgccctgag ttgccacggc acgggggaag
      541 aactccgtcg tgcccgcgaa cggggagtcc cggtcatcgg tatatgcggg ggctaccaga
      601 tgctcgggag ccggatcgtc gacgccggga ccgagtcggg gaccccgggc gagcacccgg
      661 ggttcggtct cctcgatgtc accaccacct tctccggcta ccacaagacg acggtgcagg
      721 tccggcgacg ggcgaccggc cccggcccca tcctctcgcg gatgggagag gtggccggct
      781 acgagatcca tatgggcgag acggagcggg ggagtacgct cgaggcgttt gccggggaag
      841 gggccgtaac ccccgacggc ctggtcttcg ggacctacat gcacggcctc tttcagaacc
      901 cgggcgccgt caatgccctc cttgcctatc tttcggagcg gcgtggggtt ccgttcgagc
      961 cggcgacggc gggcggcgac gccgcctacg acgccctcgc ccggcacttc gaggagcacg
     1021 tggatatgga tgcgatcgtg gcctggtttg gggacgggga ggagtagggg gccggcgctc
     1081 gcatcatcgc ccactcctgc acgaggctct ggcatcctct atcgccaacg cgggaggcga
     1141 ctggtgtggg taacgataca gccccacgcg aagatgcaac ggagagagac ctcagagggg
     1201 ggcgatcagg gagagctgct cgactatgag cagatccagg taatcggtta cggggacgac
     1261 gtcccacgcg caggcctcga acgcgatatg ctcgatatcc acgccgggag ggtgctcttc
     1321 acgtatgata gagccgagcg catccacctg cccgggctcg ccctcgtact gcaccaccac
     1381 ctgttcggcg ccgttctgca cctggatctt tgcgtaaaac ctctggaatc cggtatcgag
     1441 ggctctttcc agcaggtaga gccggtaacc tacatcatgc actcccctgc cggtgatgat
     1501 cgcccgcacc ttcatagaag ggcatctctc cggaaaggtc atataccctc cggagcggct
     1561 acgaacacct gcgatccggc gctccggccc gtacggatca tcacgccacg aaccggcctt
     1621 cgaaaaaaag agactctgcc ccggctcttc agatgaagaa ccggaacgct atccaggcga
     1681 tgagcaccat gatcgcggag tacttcagcc ccgcaaggac ccggccctcg cccatgacgc
     1741 ccgccgcgag ccccgagaag aacccctgga tcagggcgga gtgggagaag agccggttgt
     1801 agaggaagag gtccacgccg cccatgaaca tctggctcga gcccgatttt gcgaccgcct
     1861 cccccgcctc ggccatcgtg gtgaggaagg tgctcgatag cacgccgatg acgaagagga
     1921 agacgaagaa cgacatgacg atgatgatca tgtagatcat catgccgttc ctccgctccg
     1981 acttcaggtt gaagaactcg taggcgtcgt gcgccgcagc ccgcagcacc tcgctcacgt
     2041 cgccgcccgc agaactcgcg tgggcgatca ggtccacgct ccgctcggca agcggagtgt
     2101 tcacgacctt cccgaaccgg cggatggcct ccacgaacgg gacgccccag gacatctcgg
     2161 tatcgagctt gcggatatgc ggcgtcagcg ccccgtactc gccctccgag acgatgtgga
     2221 tggcgtgcgg gagcgtcatg cccgagtcgt tcataccggc cacgtcgcgg aagaagttcg
     2281 ggagcgaggc ctcgatgctc gatacccgga gcgcctcctt caggtcgagg gccgcaagcg
     2341 ggacgatggc gatgaggaga gcaaagacga atatgtcgtc gaccatcgtc gtcgtgagga
     2401 ggatgccggg accatacgac atcaccaggc tcgcggaccc gccgatgagg aggaagaggg
     2461 caaccgggac ggatacgaag aggacggtga cggggtcctc gatcatcacc cggagcgggt
     2521 gccggatgaa ccggtagaac ccttcctggt acttccgccg gttctcgatc cgcgaaaata
     2581 tctcccgttc cccaaacggt tcgtccgcaa aagattcctc cgccgcggga ccccggggtt
     2641 ggttcctgtt caggaaatca tcgatgatct ctttgaatgc catatcacac ctccggggtc
     2701 atggaactga tcaggatgac gaacatgatg ctcccgaacg gcacgatcag gtagatgatg
     2761 atgtagagga agaacgggtt tgcatcgccc gagaggatcg tcatcaccga ctgcaggatg
     2821 atcaaaaaga gcatgccggc aaccatcgcg gtgacgtagg actccgcgat caggccgagc
     2881 gtctcaagga acgtcttctg ctgctggttg ttctcaagcg tatactgtcg ggccttggcg
     2941 cggaagtact ccgtgaggtt gctcccgctc gatatgcttg cgaccgcccc ctgcaggaac
     3001 tcccgcatcc gctcgctcgg cgtcgcctgc gagaccgacc ggagggcgtc aagaagatcc
     3061 ttgccgaaga aatccgtctc tcgggcgacg taacgcgcct cgacggcgct ctcgccgtat
     3121 atcctgctct ggccgagcag ccggaagacc tcgtcggggg tgatccccgc cgaagacatc
     3181 gcggtgacgt agttgatggc gtagggcagg gtggcgtcga tgtttctccg gcgctctccg
     3241 gcccgtatgc cgggatagag gaggaagatc aggtaggtta tccctccgaa tatcaggagg
     3301 gagaggagcg tgatgacgac ggtgccgatc aggagtttgt aggcgttcaa cgcgtagaag
     3361 acctccggga ccgcgccgtg atacgatatc atctcgggaa cccggagagc gtaggtcaga
     3421 atcccgatga gcgcggcggc gaccagcccc acggcgatcg aggagacgta ggcggtcgcg
     3481 aggtaggcct caaacggcgt attcatccgg gcccccgtca ggtcgtttcg gaggtcgatg
     3541 tactccccgc gcctcgcctt caggtcccgg ccgagcaggt tgaaacagaa ccgttcatac
     3601 ccgttcatgc cggactcccc tccccgtaga ggtcggtgcg cacacgctcg atgaccgact
     3661 ccgggtcacg gaagtaggag accaggatct tgctcacgtc gcggaagtgg cggatcttct
     3721 tgatgcgcat ccattcaagc acttcctggc gccgtttcag ctcctcgcgc attcgctcct
     3781 cgctccagcc ccggtcctcc atgatctgct cgagtatgta ggacttcccg gagtagcgga
     3841 tctcgtcggt cgccgggtgc cacctgaaga cctcgttcgt gatcagttcg ttcgtccggg
     3901 ggtcgatatc cagtatctcg atgagctgct tgttcctccg gatccgctgg ccgcccaccc
     3961 gggcctgcac ctggatggac accagggaga gagccgagag catgttccgc ggcacgtcga
     4021 tcggcgggtt ttccaggcgg tgaacggcgc ttgcgaccga atccgcgtgc atcgtcgcgt
     4081 aggtgacgtg tccggtgctc atcgcctgga agagggtcag ggcctcgcgg ccgcggacct
     4141 cgccgaccag gatatactcg gggcgctgcc ggagggcggc acgcaggagc tcgtacatat
     4201 cgatctcgc
//
LOCUS       DAOSEE010000169         4172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_7527_length_4172_cov_254.847462, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000169 DAOSEE010000000
VERSION     DAOSEE010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7527_length_4172_cov_254.847462"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..1011)
                     /gene="fen"
                     /locus_tag="PLI76_10105"
     CDS             complement(10..1011)
                     /gene="fen"
                     /locus_tag="PLI76_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease-1"
                     /protein_id="HOI62427.1"
                     /translation="MGVAIRDILADCKEPLAWNDLSGIAAVDAHNALYQFLSIIRQPD
                     GTPLMNGAGRVTSHLSGILFRTVNFLEKGIRPVFVFDGRPPEFKQGTINERREIRARA
                     DEAWKTALAEGDMEEAYKQASASARIDGHILESSRELLDLLGIPRVQAPSEGEAQAAH
                     MARRAGVAYAVSQDYDSLLFGTPVLVRNLTISGRRKMRGRTITVNPERIVLASLLDRL
                     GVTREQLVDIGILVGTDFNPGIRGVGGKTALKIVRKGEFASVIAEKQPDFDPDPIREF
                     FLDPPVTDDYTLEWRPPDVEGVVSMLCGRYDFSEDRVRSALARAPAKGTQKTLDAWF"
     gene            complement(1060..1374)
                     /locus_tag="PLI76_10110"
     CDS             complement(1060..1374)
                     /locus_tag="PLI76_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62428.1"
                     /translation="MEIRELIGDIVLTIVMVVSTVVLVMRFWQDLTIAVAATLMMLSL
                     GGLLISLGMKIVSLERSVAQRERTMRVNIEEMEKTMSARYDNTISHIEGIVDGLSRRM
                     YR"
     gene            1432..2343
                     /locus_tag="PLI76_10115"
     CDS             1432..2343
                     /locus_tag="PLI76_10115"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042500 - aspartic endopeptidase activity,
                     intramembrane cleaving [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presenilin family intramembrane aspartyl
                     protease PSH"
                     /protein_id="HOI62429.1"
                     /translation="MQVRDWLSLLGMPLMLLSVQVIAVVLVLPMQAAGLAAFEDPESV
                     ANPLIFIGMLLVFTLILLLLIRFGGRRFIALFIGFAIFMTFLYIFSALSLLVLGATDA
                     AAVGAIIAAAAATALLYLYPEWYVIDVLGVLISAGVASIFGVSLAILPVLVLLVLLAI
                     YDAISVYRTKHMITLAEGVLETKAPIMVVVPKRADYSFRREGLNISDGEERGAFVMGM
                     GDLIMPTILVTSSHVFVDAPAVLWTLSAPTLGAMAGSLAGLAVLLRFVNRGNPQAGLP
                     PLNGGAILGFLVGAVLAGSFGWCPFCI"
     gene            complement(2276..3256)
                     /locus_tag="PLI76_10120"
     CDS             complement(2276..3256)
                     /locus_tag="PLI76_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOI62430.1"
                     /translation="MEFETIPTVPTADEVLDRSLRRAAAKKKQKINIDRANEEFVRAV
                     ASAIHDKLKSVVASFPSFERLPPFYQEAADILVSLDRLKKSLGAVTWAADQVRTIGSG
                     YARSMRSAEETDRVRRQAVARIASVVHQVEDDLLFLNEARNILRKLPHVNEGEFTVVV
                     AGYPNVGKSSFIRLVSTAEPEIAAYPFTTKGVIVGHRDIGRRERIQFIDTPGVLERPA
                     DERNPIERQAVSAIINTADVVLFILDASEHCGYTLDDQVRLLEEVRGFVDVPVVAVAN
                     KADIRGIEGCPAMSTLTGEGVEAALDLLLIYRKDTTQTNLREPPQPGTQE"
     gene            3305..>4172
                     /locus_tag="PLI76_10125"
     CDS             3305..>4172
                     /locus_tag="PLI76_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOI62431.1"
                     /translation="MDLSPIQKDILITLITLYHQSSHSIKGEEIADVLKRNPGTVRNQ
                     MQALKALGLVDGVPGPKGGYSPTARAYKELNLADLEHQSEVPIFRDGEKVKGVRVSEI
                     GFTTLCHPDLCHAMVKIIGSVKLFKVGEMLSIGPTPVNKLLIRGEVFGMDENQQALMI
                     SISEMISLPKQAIKHYMSTPLLTLPLSATLRDAVHLFNTHHIHGAPVMEDGNLAGIVT
                     LSDIARALDQGQTLDAPVSGVMTTDVIEAPSSIRLYELVGRFKEREIGRLIVVEGGKP
                     VGIVTQTDIIRVF"
BASE COUNT          749 a         1292 c         1251 g          880 t
ORIGIN      
        1 ggcgcctcgt cagaaccagg catcgagcgt cttctgcgtc cccttcgccg gggccctggc
       61 aagagcgctc ctcacccggt cttcggagaa gtcgtagcgt ccacagagca tggagacgac
      121 cccctcgacg tccggcggcc gccactcgag ggtgtagtcg tcggtgaccg gcggatcgag
      181 gaagaactcg cggatcggat cagggtcgaa gtcgggctgt ttctcggcga tgacagatgc
      241 aaattcacct tttcgaacga tcttcagggc cgtcttgccg ccgacgcccc ggatgccggg
      301 gttgaagtcc gtgcccacca ggatgccgat atcgacgagt tgctccctcg tcacgccgag
      361 gcggtcgagg agagaggcga ggacgatgcg ctcgggattc accgtgatcg tccgtccccg
      421 catcttccgt ctcccgctga tcgtgaggtt ccgcaccagg accggggtgc cgaagaggag
      481 cgagtcgtag tcctgcgaga ccgcgtaggc gacacccgcc ctccgcgcca tgtgggcggc
      541 ctgcgcttcg ccttcgctcg gcgcctgcac ccgggggata ccgagcaggt ccaggagctc
      601 gcgggacgat tcaagtatat ggccgtcgat ccgggcggat gcgctcgcct gtttgtacgc
      661 ctcctccata tccccctccg caagcgcggt cttccaggcc tcgtcggccc ttgcgcggat
      721 ctcgcgccgc tcgttgatcg tcccctgctt gaactccggc ggtcttccgt caaagacaaa
      781 gaccggccgg atccccttct ccaggaagtt caccgtccgg aagaggatgc cggagaggtg
      841 cgaggtgacc ctccctgcac cgttcatcag cggggtgccg tcaggctgcc ggatgatgga
      901 gagaaactgg tagagcgcgt tgtgggcatc cacggcggcg atgccggaga ggtcgttcca
      961 cgccagcggt tctttacagt ctgcgagaat atcgcgaata gcaacgccca tgctatctac
     1021 tcatgagaga gggagatacc attaagatgg cggtccggtt cagcggtaca tccggcggga
     1081 gagcccgtcg acgatcccct cgatatggct gatcgtgttg tcatacctgg cgctcatcgt
     1141 cttctccatc tcctcgatgt tcacgcgcat ggtgcgctcc cgctgtgcaa cgctccgctc
     1201 gaggctcacg atcttcatgc cgagagatat gagcaagccc ccaagagaga gcatcatgag
     1261 cgtcgcagca accgctatgg tcaggtcctg ccagaaccgc atcaccagca cgacggtgga
     1321 gaccaccatc acgatggtga gaacgatgtc gccgatgagt tcgcgaatct ccatatttta
     1381 aagtgatgag gatgcattaa ttaacctacc taaaatgact ggagacatat gatgcaggta
     1441 cgtgactggc tatcgcttct tggaatgccc cttatgctcc tctccgtcca ggtgatcgcc
     1501 gtcgtcctcg tgcttcccat gcaggcggcg ggtcttgcgg cgttcgaaga tcctgaatcg
     1561 gttgcaaatc ccctgatatt tatagggatg ctgctcgtct tcacgctgat cctgctcctc
     1621 ctgatacggt tcggaggccg gcgcttcatc gccctcttca tcgggtttgc catattcatg
     1681 acattcctct atatcttctc ggcgctctcg cttcttgtgc tcggggccac cgatgccgcc
     1741 gccgtcggag cgatcatcgc tgccgcggcg gcgacggcgc tcctctatct ctaccccgag
     1801 tggtacgtca tcgatgtcct cggcgtcctg atatccgcgg gggtcgcatc gatcttcggg
     1861 gtctcccttg ccatcctccc ggtccttgta ctgctcgttc tgctcgcgat ctatgatgcc
     1921 atatcggtct accgcacgaa acacatgatc acgcttgcgg agggcgttct cgagacgaaa
     1981 gccccgatca tggtcgtggt cccgaaacgg gcggattact cgttccgaag agaggggctc
     2041 aatatcagtg atggagaaga gcgcggcgcg ttcgtcatgg gcatgggcga cctcatcatg
     2101 cccaccatcc tcgtcacgtc gtcgcacgtc ttcgtggatg cacccgccgt cctctggacc
     2161 ctctccgccc cgacgctcgg cgcgatggcg ggatcgcttg ccggccttgc cgtcctcctc
     2221 cgcttcgtca acaggggcaa cccccaggca ggcctcccgc ccttgaacgg cggcgctatt
     2281 cttgggttcc tggttggggc ggttctcgca ggttcgtttg ggtggtgtcc tttctgtata
     2341 tgaggaggag atcgagcgcc gcctccacgc cctcgccggt gagcgtggac atcgcggggc
     2401 agccttcgat cccccggata tccgccttgt ttgcgaccgc caccaccggg acatcgacaa
     2461 acccgcggac ctcttcgagg agccggacct gatcgtcgag cgtgtagccg cagtgctcgc
     2521 ttgcatccag tatgaagagc acgacgtcgg ccgtgttgat gatggcgctc accgcctgcc
     2581 gctcgatggg gttcctctcg tcggccgggc gttcgaggac accgggcgta tcgatgaact
     2641 ggatccgttc gcgccgcccg atatcgcggt ggccgacgat gacccctttg gtggtgaagg
     2701 ggtaagcggc gatctcgggt tccgccgtgg agacgagcct gatgaacgac gatttcccga
     2761 cgttggggta tcctgccacg accacggtga actcgccctc gttcacgtgc ggaagttttc
     2821 ggagtatgtt tcgcgcttcg ttcaggaaga ggaggtcatc ctccacctgg tggacgacgg
     2881 aggcgatgcg ggcgacggcc tgccgccgga cccggtcggt ctcctctgcg gatcgcatgc
     2941 tgcgggcgta gcccgaaccg atggtccgga cctggtcggc cgcccaggtg acggcgccga
     3001 gagacttctt caaccggtcg agcgagacga gtatgtcggc cgcctcctgg taaaagggcg
     3061 ggaggcgctc gaagctcggg aacgacgcga ccacgctctt gagtttatca tggatggcgc
     3121 tcgcgaccgc tcttacgaat tcctcgttcg cacggtcgat gtttatcttt tgcttctttt
     3181 tggctgccgc ccggcgaaga ctgcggtcga gcacctcatc tgccgtcggg acggtcggta
     3241 tagtttcaaa ctccactgca cgcacaacct aatctattta aacaacgcat tatgagtgat
     3301 aattatggat ctttccccaa ttcagaagga tatcctgata accctgatta ccctgtatca
     3361 tcagtcttct cactctataa aaggtgagga gatcgcggat gtgctgaagc gcaaccccgg
     3421 caccgtccgc aaccagatgc aggcattaaa agcgctcggg cttgtagacg gcgttccggg
     3481 cccgaaaggc ggttacagcc cgaccgcacg ggcatacaag gagctcaatc ttgccgatct
     3541 cgaacaccag tccgaggtgc cgatctttcg cgacggcgag aaggtgaaag gtgtccgggt
     3601 ctcggagatc gggttcacca cgctctgcca tcccgacctc tgtcatgcga tggtcaagat
     3661 catcgggagc gtgaagctct tcaaggtagg cgagatgctc tcgatcggac ccacgccggt
     3721 gaacaaactc ctcatccgcg gcgaggtctt tggaatggat gagaaccagc aggctctcat
     3781 gatcagcata tcggagatga tatcgctccc gaagcaggcg atcaagcact atatgagcac
     3841 cccgctcctg accctgccgc tctccgccac cctccgtgat gccgttcatc tcttcaacac
     3901 gcaccatatt cacggggcgc cggtgatgga ggacggcaac cttgcaggca tcgtgacgct
     3961 gagcgatatc gcccgcgccc tggatcaggg ccagacactg gatgcgcccg tttccggggt
     4021 catgaccacc gacgtgatag aggcgccgtc gtcaattcgg ctctacgagc tcgtgggccg
     4081 gtttaaggaa cgggagatag ggaggttgat tgtagtggag ggcggcaagc ccgtcggtat
     4141 cgttacccag accgatatta ttcgggtctt tc
//
LOCUS       DAOSEE010000170         4149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_7579_length_4149_cov_249.255007, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000170 DAOSEE010000000
VERSION     DAOSEE010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7579_length_4149_cov_249.255007"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PLI76_10130"
     CDS             <1..296
                     /locus_tag="PLI76_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867598.1"
                     /note="Sec61alpha; forms heterotrimeric complex in the
                     membrane; in archaea the complex is similar to the
                     eukaryotic complex and consists of Sec61alpha which forms
                     the channel pore and Sec61gamma and Sec61beta; the beta
                     subunit is not essential; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOI62432.1"
                     /translation="KDVARQIQRSGMHIPGYRRNEQVLVRYLDRYIPRITIIGGAFIG
                     VLSVVANLFGVVGAVGGTGLLLAVSITYRLYEEVASQQIMEMYPFMRGFFGKE"
     gene            459..1034
                     /locus_tag="PLI76_10135"
     CDS             459..1034
                     /locus_tag="PLI76_10135"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOI62433.1"
                     /translation="MGKKVVVTGVPGVGKTTVINGAMERLAAEGIAYEAVNFGSFMFD
                     VARKENLAADRDEMRKLGKDVQKRLQQAAASEIAAMSADANIIVDTHSSVKTPAGFLA
                     GLPEWVLKELMPDIVVLVETDPDQILMRRLGDASRTRDMEGARAIAEHQEFNRAVSAA
                     YAMYTGCTVKVVRNENYLLERGIDDLADVLR"
     gene            1041..1661
                     /locus_tag="PLI76_10140"
     CDS             1041..1661
                     /locus_tag="PLI76_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EMC3/TMCO1 family protein"
                     /protein_id="HOI62434.1"
                     /translation="MVDLKKHGPTIALLFTMLVMLSYGIEGVRVTVGTMMNVLLGPFI
                     DVLGVPFFVMIMILSSITGLYSSLVQKYTIDYEKMQETQAKMRVFQKEFREAQLSGDE
                     KRVKKLQGKQERMMQDQLDMSRQQFTPMAIILILSVPIFFWLLLRLPAVGTPIDIASG
                     IVMPFIGTVTLSSIAFWIVPAWILWYMLCSLTISQVIRKALNIGGL"
     gene            1661..2203
                     /locus_tag="PLI76_10145"
     CDS             1661..2203
                     /locus_tag="PLI76_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOI62435.1"
                     /translation="MRITISGPPGSGTTSLARYLAGKYDLAFISAGEVFRQLAREHDM
                     ELAEFGRFAENDPAVDRMIDARQKEIGEGSEKIIVEGRLSGRMIENADLRIWLSASIS
                     CRAKRIAGRDGMDEEEARVYTENRQRSEATRYRSYYGIDIGDLSPYDIVLSSETFGVD
                     ALAAIVDTAIACLTRQKGSL"
     gene            complement(2200..2844)
                     /locus_tag="PLI76_10150"
     CDS             complement(2200..2844)
                     /locus_tag="PLI76_10150"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOI62436.1"
                     /translation="MPIDDIKPTRAGLLIVRRRMALAERVHELLSMKLDGMMLELIRL
                     TDRAAEQRRELEDLYTGAREMAAVAAMMEGATGVLLAALSVEAVPTYAVGHKNVFGVR
                     LPDLEPVAVKKTLDERGYGVLGTSSVIDDAADAYENLIEAIIASAELEGGVRQLLGEI
                     ERTRRRVNALEFKVIPELMEKRRFIEYQRDEMERQEWTRLRRIKKIKAKRAEKR"
     gene            complement(2854..3978)
                     /locus_tag="PLI76_10155"
     CDS             complement(2854..3978)
                     /locus_tag="PLI76_10155"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HOI62437.1"
                     /translation="MALRGMISPSYIYACTRFRVRRTKLLPRDAYRRIMQMGIPEIVT
                     HLGRQEEYQEIMDLAGDFSGARLIEEAVNRNLARSFRGAMAIAPGSLHTLTGEYLARW
                     DIANVMAILRGTVHHIPRQQIRDLLIPAGELDRALLDRLLSFTTCGEALDALQGWRLY
                     PILAEHYRVCGEEGAFARIEDELYQRYYAGLLGLARSGCSGCQELIPYLRFEIDITNM
                     KNLLRLHCAREACDITLVDQTMIPGGNMSIDLLRRLYGAGTEAEFTSIFLQTTIVPVL
                     ARAVRELRQDPNFSSTDAAELVWRRWHQHLRPVHEIEMAITRIRLQQMDALSRRYPFS
                     VLPVLAYLERKRYEVANLRAIARGKAFGLPADRIWQYIVL"
BASE COUNT          754 a         1241 c         1346 g          808 t
ORIGIN      
        1 caaaggacgt ggcccggcag atccagagga gcgggatgca tatccccggc taccgtcgga
       61 acgagcaggt gcttgtgagg tatctcgacc gctacatacc gaggatcacc atcatcggcg
      121 gtgcgttcat cggggttctc tcggtcgtcg caaacctctt cggtgtggtc ggcgcggtcg
      181 gcggaacggg tctgctgctt gcggtgagca tcacctaccg gctctacgag gaggtcgcga
      241 gccagcagat catggagatg tacccgttca tgcggggatt cttcgggaag gagtgagtcc
      301 gcccggactc acccttcttt gaaatattgc cgttacatcc ttttttttgt cccgggcaag
      361 tccggtcgcc ggtccgggtc gggttgattc cgaaaggcat tacttaggtg agcgcggatt
      421 tatcctgcag ggaagataac ataagtggag tgatagagtt ggggaagaaa gtcgtcgtaa
      481 cgggggtccc cggtgtcggg aagaccaccg tcatcaacgg tgcgatggag cgcctggcgg
      541 ccgaaggtat cgcctacgag gccgtcaact tcggttcgtt catgttcgac gtggcgagga
      601 aagagaacct cgccgcagat cgtgacgaga tgcgtaaact cggaaaagat gtccagaagc
      661 gccttcagca ggcggcggcg tcggagattg ccgccatgag cgccgatgcg aacatcatcg
      721 tcgataccca cagcagcgtc aagaccccgg cggggttcct cgcgggactg cccgagtggg
      781 tgctcaaaga gctgatgccg gatatcgtcg tgctggtgga gaccgatccg gaccagatcc
      841 tgatgcgccg tctcggcgac gcttcgagga cccgggatat ggagggagcg agagcgatcg
      901 ccgagcacca ggagttcaac cgcgcggtca gtgcggcgta tgcgatgtat accggttgca
      961 ccgtcaaggt cgtccgaaac gagaactacc tcctcgagcg gggcatcgac gacctggcag
     1021 acgtgctgag gtaacctgac atggtggacc tgaagaagca cggcccgacg atcgctctcc
     1081 tcttcaccat gctcgtgatg ctctcctacg gcatcgaggg ggtgcgggtg acggtcggca
     1141 cgatgatgaa cgtactgctc gggccgttca tcgacgtcct cggcgtgccg ttcttcgtca
     1201 tgatcatgat cctctcgagc ataacgggcc tctactcgtc gctcgtccag aagtacacca
     1261 tcgattacga gaagatgcag gagacgcagg cgaagatgcg ggtcttccag aaggagttcc
     1321 gcgaggccca gctctccggg gacgagaaga gggtcaagaa actccagggg aagcaggagc
     1381 ggatgatgca ggaccagctc gatatgtcca ggcagcagtt tactccgatg gcgatcatcc
     1441 tcatactctc ggtgcctata ttcttctggc tcctcctgcg gctcccggct gtcggcacgc
     1501 cgatcgacat agcgagcggt atcgtgatgc ccttcatcgg gaccgtcacg ctctcgagta
     1561 tcgcgttctg gatcgtgccg gcctggatcc tctggtacat gctctgctcg ttgacgatca
     1621 gccaggtgat ccggaaagcg ctcaacatcg ggggactctg atgcggatca ccatcagcgg
     1681 ccctcccggg agcggcacca cgtcgcttgc ccgctacctc gccgggaagt acgaccttgc
     1741 cttcatatcg gcaggggagg tcttccggca gcttgcccgg gagcacgata tggaacttgc
     1801 cgagtttggc cggtttgcgg agaacgaccc cgcggtcgac cggatgatcg acgcccggca
     1861 gaaagagatc ggcgaaggat ccgagaagat catcgtcgag ggcaggctct cgggccggat
     1921 gatcgagaac gcagacctcc gtatctggct ttctgcgtcg atatcctgcc gggcaaagag
     1981 gattgcgggg cgtgacggta tggatgagga ggaggccagg gtctacaccg agaaccggca
     2041 acgctcggaa gccacccgct accggagtta ctacggcatc gatatcggcg acctctcgcc
     2101 gtacgatatc gtcctctcat ccgagacctt tggcgtggac gctcttgccg cgatcgtgga
     2161 tacggcgatt gcgtgcctta cgcggcagaa aggctctctc taacgttttt ccgccctctt
     2221 tgcctttatc tttttgatac gccggagccg cgtccactcc tgccgctcca tctcgtcgcg
     2281 ctggtactcg atgaaccggc gtttctccat gagctccggg atgactttga actccagggc
     2341 gttcaccctc cggcgggtcc gctcgatctc gccgaggagc tgcctgacgc ctccttcgag
     2401 ttcggcgctt gctatgatcg cttcgatgag gttctcgtag gcatcggcgg cgtcgtcgat
     2461 gaccgatgat gtcccgagga cgccgtatcc ccgttcgtca agcgtcttct tcaccgctac
     2521 cggttccagg tccgggagcc gtacgccaaa gacgttcttg tgcccgaccg cgtaggtcgg
     2581 tacggcctcc accgagagcg cggcaaggag cactcccgtc gccccctcca tcatggcggc
     2641 gacggctgcc atctcccggg ccccggtata caggtcttcg agttcccggc gctgttctgc
     2701 ggcccggtcg gtgagcctga tcagctccag catcataccg tcgagtttca tcgagagcag
     2761 ttcatgtacc cgctccgcga gcgccatccg ccgcctgacg atcaggaggc cggcccgggt
     2821 aggtttgatg tcgtcgatgg gcatgatcct tcctcacagc acgatatact gccatatccg
     2881 gtcggccggc aggccaaacg ccttcccccg cgcgatcgcc cgcaggttcg cgacctcgta
     2941 tctcttccgt tcaaggtagg cgagcaccgg caggaccgag aacgggtagc gccgggagag
     3001 ggcatccatc tgctggagcc tgatacgggt gatcgccatc tcgatctcgt gaacgggccg
     3061 caggtgctgg tgccatcgcc gccagaccag ttctgcggcg tccgtgctcg agaagttcgg
     3121 gtcctgccgg agttcgcgca ccgcccgggc cagaaccggg acgatagttg tctgcagaaa
     3181 aatgctggtg aactctgcct ctgtcccggc gccgtagagc cggcggagga gatcgatgga
     3241 catgttgccg cccgggatca tggtctggtc gacgagggtg atgtcgcacg cttcccttgc
     3301 acagtggagc cggaggaggt tcttcatgtt ggtgatgtct atctcgaacc ggaggtaggg
     3361 gatcaactcc tggcaaccgc tgcaccctga tctggcaaga ccgagcagcc cggcgtagta
     3421 gcgctggtag agttcgtcct cgatccgggc aaaggcacct tcctccccgc agacccggta
     3481 gtgctctgca aggatcgggt agagtcgcca gccctgaagg gcatcgagcg cctcgccgca
     3541 ggtcgtgaag gagaggaggc ggtcgaggag cgcgcgatcg agttcgcctg ccggtatgag
     3601 gaggtcgcgg atctgctgcc tgggtatgtg gtgaaccgtg ccccgcagga ttgccatgac
     3661 gtttgcgatg tcccaccggg cgaggtattc tcccgtcagg gtgtggagac tcccgggagc
     3721 gatcgccatg gcaccccgga acgatcgcgc caggttgcga ttaaccgcct cttcgatgag
     3781 ccgggcgccg gaaaagtcgc cggcgagatc catgatctcc tggtactcct cctgccggcc
     3841 gaggtgggtg acgatctccg gtatgcccat ctgcatgatc cggcgatacg catcgcgggg
     3901 gaggagcttt gtcctgcgga cgcggaaccg ggtgcaggcg tagatatacg acggagagat
     3961 catgccgcgc agggccatgt accactatta tcgtcccacg tataaatgcg cacccggccg
     4021 atcgggggcg gggggtccgg caggggcacg tccgggccct gccggcgaat cggagggcgg
     4081 cacctgccgg gtggctccgg ggtcttgaag agcagcggcg atagcccggc ccggataccc
     4141 gcggccggg
//
LOCUS       DAOSEE010000171         4134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_7608_length_4134_cov_210.226036, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000171 DAOSEE010000000
VERSION     DAOSEE010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7608_length_4134_cov_210.226036"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..1420
                     /locus_tag="PLI76_10160"
     CDS             122..1420
                     /locus_tag="PLI76_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOI62438.1"
                     /translation="MSKTLVVGITLLLLCIGISAVSAEIGGDQGWYRIHCNVDGASVY
                     FDGEYKGAISGGELYVAVYTTGTPYSTIRVEKSDYRTYTGSLPNTPIMGDTAEVYATL
                     QPAETYGSIYVSSSPSGAAIYLNGNYRGVAPLTIRDLTPGTYNVEADLSGYQSDWSRV
                     TVQSGKQSNVQFTLTPIQQYGSVSITSSPSGAYVYMDGVYKGRTPLTLNNVAANNHNV
                     ELDLSGYYDWRTTVSVASGVTRYVDARMVAIPSETTGRIDVASSPTGADVYLDGVHQG
                     KTTSTGPFTISNVRVGTHTVRVAMSGYHDYTTSVDVGGATTSYISAALQPASMGATGS
                     IAVASSPSGAEIYIDNAYRGITPLTVDGISAGTHTVMVALNGYNDWSTSVQVGAGSIA
                     SASASLSPVSAPTAKAGVAPFAAIGALAALGLLGARRKQN"
     gene            1570..3078
                     /locus_tag="PLI76_10165"
     CDS             1570..3078
                     /locus_tag="PLI76_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HOI62439.1"
                     /translation="MEKSLDLINARIRDGSARVVTAEEMPAIVDALGEEGALAEVDVV
                     TTGTFGAMCSSGAFFNFGHADPPIRMERVWLNDVEAYAGIAAVDAYLGATQEADTQDR
                     VYGGAHVLEELVAGGTVELRATSHGTDCYPRRSITTDLLLEDMNQAIMLNPRNSYQRY
                     NAATNTTDRTLSTYMGTLLPRCGNVSYSGAGTLSPLANDPNFRVIGSGVPIFLAGAEG
                     MVVGEGTQHSSGGGFGTLMVTGDMKRMRQEFLRAAVMPGYGVTIYVGVGVPIPVLDTG
                     IVRSTAVRDEDILTDVIDYGTPRRDRPSMAKVSYADLRSGSIDIGGEAVRTSSLSSYR
                     RAREVARELKTWVEQGKMELTLPVRRLDPAKRAKPMRETAITPRVRDIMNRQVISIAE
                     DEEIRAAAKRLLRGETNHLPVLAGDGTLVGIITTYDVSKAVVTDGRLRQVKDIMTRQV
                     IKTTPDEPVDIAARKLEQNNISALPVVDATNRVVGILSAIDLGKLFGGRRRR"
     gene            3075..3473
                     /locus_tag="PLI76_10170"
     CDS             3075..3473
                     /locus_tag="PLI76_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOI62440.1"
                     /translation="MKLLLTYSRRGQSDPGRKPVIARIVKETGILVNVEKANIDSTAG
                     EVLIDIPDADADLVRRCLEEMGVAVRVMEDAVTLDEAECVDCGACISICPQEVFSFDD
                     EWRLRVSAARCVLCGKCIRACPHSALSLQG"
     gene            3470..>4134
                     /locus_tag="PLI76_10175"
     CDS             3470..>4134
                     /locus_tag="PLI76_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HOI62441.1"
                     /translation="MIREHFEFRQTIATILADEPRHVAAAKAGMLAARHEVERQIALD
                     PYFAATFEPYAPKDPERTVARMVRAAGEAGVGPMAAVAGAIARAGVEAMVEAGASFAL
                     IDNGGDIALVSDRDLTIGVYAGASSLSGRIAFRLSPQMDILGICTSSATVGPSISFGV
                     ADAVTVFAPDPAVADAWATAICNRITADDTSVLDALPGTGVRGVLAIIDDAVIRHGDL
                     PRIV"
BASE COUNT          777 a         1398 c         1296 g          663 t
ORIGIN      
        1 ccatcgagcg gagaaaattc tgcagttttg ctttaagtct atggagatag agtatatcgt
       61 atagaaataa ttatccgtga ctaaaccgac ctaccactca ttgtttatcg gagaaccgat
      121 tatgtcaaag actcttgttg tcggaataac gcttcttctc ctctgcatcg gcatctctgc
      181 cgtatccgca gagatcggag gagaccaggg ctggtatcgc atccactgca acgtcgacgg
      241 cgcctccgtc tacttcgacg gagagtacaa aggcgccata tccggaggcg aactctacgt
      301 cgcggtctac accaccggga cgccctacag caccatccgg gttgagaaga gcgattatcg
      361 gacctacacc ggcagcctgc cgaacacccc catcatgggc gataccgcgg aggtctacgc
      421 aaccctgcag ccggccgaga cctacggctc gatctacgtc tcgtcgagcc cctccggtgc
      481 cgcgatctac ttaaacggca actaccgggg cgttgcgccg ctcacgatca gggacctcac
      541 ccccggcacc tacaacgtcg aggccgacct ctcgggctac cagtccgact ggtccagggt
      601 cacggtccag tcgggaaagc agtcgaacgt ccagttcacc ctgacgccca tccagcagta
      661 cgggtcggtc agcatcacct caagcccctc gggcgcctac gtctacatgg acggggtcta
      721 caagggccgc acccccctga cgctcaacaa cgtcgcggcg aacaaccaca acgtcgagct
      781 cgacctctcc ggctactatg actggaggac gaccgtctcc gtcgcgtccg gcgtgacccg
      841 gtacgtcgat gcccgtatgg tcgccatccc ctccgagacc accgggcgca tcgacgtcgc
      901 ctcctctccg acaggcgcgg acgtctatct cgacggcgtc caccagggga agacgacatc
      961 gacgggaccg ttcacgatat cgaacgtccg tgtcgggacc cataccgtga gggtcgccat
     1021 gtccgggtac cacgactaca ccacatcggt cgacgtcggg ggagcgacga cgagctacat
     1081 cagcgcggcc ctccagcccg cctcgatggg cgcgaccggc tccatcgcgg tcgcctcctc
     1141 tccatcgggc gccgagatct acatcgataa cgcctacagg ggcatcaccc cgctgaccgt
     1201 cgacggcata tccgccggca cccatacggt catggtggcg ctcaacggct acaacgactg
     1261 gtcgaccagc gtccaggtcg gcgccgggag catcgcatcg gcctcggcat ccctctcgcc
     1321 ggtgtcggcc ccgacggcga aggcgggcgt ggcgcccttt gccgcaatcg gtgcgctcgc
     1381 cgccctcgga ctcctcggag cccgacgaaa acagaactaa aacactctct ttttttatta
     1441 tccgggcgcg aggtgcgtcc ctcccgggac cgtgaaggaa accggagccg gctatccccg
     1501 tgccggagca ccgtattacc gaaagtaata gtcaacacct ataaatattc cagaatagat
     1561 atacgtttca tggaaaagtc ccttgacctc atcaatgccc ggatacgcga cggtagtgca
     1621 cgagtggtta ccgccgaaga gatgccggcc attgtcgacg cactcggaga ggaaggggca
     1681 ctggcggagg tcgacgtcgt cacgaccggg acgttcgggg caatgtgctc ctccggtgcc
     1741 ttctttaact tcgggcacgc cgaccccccc atcaggatgg aacgcgtctg gctcaacgac
     1801 gtggaagcct acgcaggtat agcggcggtc gacgcctacc tcggcgcaac ccaggaggcc
     1861 gacacccagg accgggtcta cggcggcgcc cacgtcctcg aagaactcgt cgccgggggc
     1921 accgtcgaac tgcgggcgac atcccacggg accgactgct acccgcgccg gagcatcacg
     1981 acggacctcc tcctcgagga catgaaccag gcgatcatgc tcaacccgcg aaactcctac
     2041 cagcgctaca acgcggcgac gaacaccacc gaccggacgc tctccaccta catgggcacc
     2101 cttctcccgc ggtgcggcaa cgtctcctac tccggcgccg ggaccctctc gccgctcgca
     2161 aacgacccca acttccgggt catcgggagc ggcgtcccga tattccttgc cggagcggag
     2221 gggatggtcg tcggcgaagg cacccagcac tcttcgggcg gcggattcgg gacgctgatg
     2281 gtgaccgggg atatgaaacg gatgcgccag gagttcctgc gggcggcggt gatgcccggc
     2341 tacggcgtga cgatctacgt cggcgtcggc gtccccatac cggtgctcga caccggcatc
     2401 gtccggagca ccgcggtgcg ggacgaggat atcctgaccg acgtcatcga ctacggcacc
     2461 ccccggcggg accgcccctc gatggcgaag gtcagttacg ccgacctcag gagcggttct
     2521 atcgatatcg gcggggaggc ggtcaggacg tcgtcgctct ccagttaccg gcgggcacgg
     2581 gaggtggcgc gggagctcaa aacctgggtc gagcagggga agatggagct tacgctcccc
     2641 gtccggcggc tcgacccggc aaaacgcgca aaacccatgc gggagacggc catcaccccc
     2701 cgcgtccgcg acatcatgaa ccgccaggtg atcagcatcg cggaggacga ggagatccgg
     2761 gcggcggcaa agcggctcct ccggggcgag acgaaccacc tcccggtcct cgccggcgac
     2821 ggcacgctcg tcgggatcat caccacctac gacgtctcga aggcggtcgt gaccgacggg
     2881 cggctgcggc aggtcaaaga catcatgacc cggcaggtga tcaagacgac ccccgatgag
     2941 ccggtggata tcgccgcccg gaaactggag cagaacaaca taagcgccct cccggtggtg
     3001 gacgcgacga accgggtggt cggcatcctc tcggcgatcg atctcggcaa actcttcggc
     3061 gggaggcgac ggcgatgaaa ctcctgctga cgtactcgcg gcgggggcag agcgaccccg
     3121 ggaggaaacc ggtgatcgcc cggatagtga aagagaccgg catcctcgtc aacgtcgaga
     3181 aggcaaacat cgactcgacg gccggcgagg tgctgatcga tatccccgat gcggatgcgg
     3241 acctcgtccg gcggtgcctc gaagagatgg gagtcgcggt ccgggtgatg gaggatgccg
     3301 tcaccctcga cgaggcggag tgcgtcgact gcggcgcctg catcagcatc tgcccccagg
     3361 aggtcttctc cttcgacgac gaatggcgtc tccgggtgag cgccgcgcgg tgcgtcctct
     3421 gcggcaagtg catccgggcg tgcccccact cggccctctc gctccaggga tgatccggga
     3481 gcacttcgag ttccggcaga cgatcgcgac gatcctcgcc gacgaacccc ggcacgtcgc
     3541 ggcggcaaag gccggcatgc tcgccgcccg gcacgaggtc gagcggcaga tcgccctcga
     3601 cccctatttt gcggcgacgt tcgagccgta tgccccgaag gatccggaga gaaccgtcgc
     3661 ccggatggtg cgggccgccg gggaggccgg cgtcggtccc atggcggccg tggccggcgc
     3721 catcgcccgg gcgggagtgg aggcgatggt cgaggccggc gcatcgttcg ccctcatcga
     3781 caacgggggg gatatcgccc tcgtgagcga ccgcgatctg acgatcggcg tctatgcagg
     3841 cgcttcatcc ttgagcgggc gtatagcgtt ccggctttcg ccacagatgg atatcctcgg
     3901 catctgcacg tcgtcggcga cggtgggccc gtcgatcagc ttcggcgtcg ccgacgccgt
     3961 gacggtcttt gcccccgacc cggccgttgc ggatgcctgg gcgacggcga tctgcaaccg
     4021 gatcacggcg gacgacacct ccgtcctcga cgcccttccg gggacgggtg tccggggggt
     4081 gcttgcgatc atcgatgacg ccgttatccg ccatggggac ctcccgcgga tcgt
//
LOCUS       DAOSEE010000172         4124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_7632_length_4124_cov_249.815188, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000172 DAOSEE010000000
VERSION     DAOSEE010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7632_length_4124_cov_249.815188"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..638
                     /locus_tag="PLI76_10180"
     CDS             24..638
                     /locus_tag="PLI76_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HOI62442.1"
                     /translation="MELDPIQMDALAELGNIGAAHAATTLSQMLMCPIDMTVPEVHAV
                     DIADMHSYIGNEISAVVVFQIQGEVADGGYIVVSMPRATVIQLTNQMLGMTDTDREID
                     DMDRSAAIEIGNIMISAFLDATAELLGIVMLPSPPALAIDMTHAAFESIVAQVAGDVN
                     DILIFNTELESEAPPVYGSIYMLPNTELMHQLFHMLERLMEPIS"
     gene            656..1147
                     /locus_tag="PLI76_10185"
     CDS             656..1147
                     /locus_tag="PLI76_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOI62443.1"
                     /translation="MENNFFSEVKAVILGLGELRVGTCPMGTIGLGSCVALILHDQRR
                     SLGGLAHIMLPESRGSVDRPGKFADTAASTLLAEMEMCGSSKFAIAATLVGGASMFEF
                     SANSLNIGERNIVAVRDVLRDQGIRIVREETGGRIGRSVYYWPEGKGRIIVKRADGTC
                     ARL"
     gene            1132..2307
                     /locus_tag="PLI76_10190"
     CDS             1132..2307
                     /locus_tag="PLI76_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62444.1"
                     /translation="MRQTLIFFFILSSLLLSPSSAVSVDVPDFHAWIASAGVTDAING
                     RYGDVIFATDTGISFYGANGTWHSVNTRFPGETAYGLLSPLDSMVTAIALDAGGHLWI
                     GYPNGLQIGDGKRYQSIQDQQFLKNLNIGCIVRWGDDMWVAAGRAGLHRYHDGAWTWY
                     KPLGPEGLGCYTVVSMAVDAASDALVVGSERDGLRVLRNRTGEVRFEPVTCDDEPVRG
                     ISEVRVDPFGGVYLFNRTTVLHYDPGGKVTPILDAGDLNPFPVAINDLAATSGGMLLI
                     ATDDGIYGWNSSGVALHIASGDGIRSNVVKKLFIDADGRCWFVVPGNVGYIPPTAGHA
                     TLDLAAAPVLAPHEPETPAIETPTVDIPSVEPAGVPDMLSGAWASFVEWVNARLAGA"
     gene            complement(2323..4095)
                     /locus_tag="PLI76_10195"
     CDS             complement(2323..4095)
                     /locus_tag="PLI76_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis/translation initiation
                     ATPase RLI"
                     /protein_id="HOI62445.1"
                     /translation="MRIAVVHRDRCHPVKCGTECILYCPRVRTGDETVVIGEDQKAVI
                     SEELCVGCGICVKKCPFEALAIVNLPEELDQPTHRYGKNGFVLYGLPIPIEGKVTGIL
                     GPNGIGKSTSVKILSGTLVPNLGRTDAPVSWKEVLDLYQGTELFDYLQVLSQKNVKVA
                     VKPQYIDQIPKVFTGSVRDLLATTDERGKLGYYIDRLSLKAVVDRPITNLSGGELQRV
                     AIAACLARDADFYFFDEITPYLDVYQRMAAADLIRELARERPVVIVEHDLAILDMLAD
                     TVHIAYGEPAVFGVITYPKGVRVGINQYLEGFLPEENVRFRPYAVTFDARAHAAGTDR
                     EVLLTFPAMTKQFTQFSLSVGGGEIRSGEVLGVLGANGIGKSTFAGLLAGVIEPDTGS
                     MDTRVRISYKPQYLKGDTDATVEEVLRQTTTKFDTSFYQHEILEPLSLSPLLQAPVNT
                     LSGGELQRVAIAVCLSRDADLYILDEPSAHLDVEQRVKISRMIRKHAEGRGASTLVID
                     HDIYVIDMISDRILVFEGEPGIRGKAVGPFDMAPGMNRFLSALGVTFRRDRSGRPRIN
                     KPGSFLDREQVAAGEYYYTEISKS"
BASE COUNT          758 a         1282 c         1325 g          759 t
ORIGIN      
        1 acaatggttt aggagagatt ttcatggaac ttgatccgat tcagatggac gcgctggcgg
       61 agttaggcaa tatcggtgca gcgcatgcag cgacaaccct atcgcagatg ttgatgtgcc
      121 cgatcgatat gacggtgccg gaggtgcatg ccgtcgatat agccgatatg cacagttaca
      181 taggcaacga gatatcggcc gtggtcgtct tccagatcca gggcgaggtc gcggacggag
      241 ggtacatcgt cgtcagcatg ccgcgggcga ccgtcatcca gctgacgaac cagatgctcg
      301 gtatgaccga taccgatcgt gagatcgacg atatggaccg gagcgccgcg atcgagatcg
      361 ggaacatcat gatatcggcg ttcctcgatg cgaccgccga gctcctgggc atcgtcatgc
      421 tgccgtcccc gccggcgctg gccatcgata tgacgcacgc ggcgtttgag tccatcgtcg
      481 cgcaggtagc cggcgacgtg aacgacatcc tgatcttcaa tacggaactc gagagcgagg
      541 cgccgcccgt ctacgggagc atctacatgc tcccgaacac cgagctcatg caccagctct
      601 tccacatgct ggaacgcctg atggaaccga tctcataacg ggcctccggg ataccatgga
      661 gaacaacttt tttagcgagg taaaggccgt aatcctgggt ctcggcgaac tccgggtcgg
      721 gacatgcccg atggggacga tcgggcttgg ctcctgcgtt gcgctcatcc tgcacgacca
      781 gaggcgatcc ctcggggggc ttgcccacat catgctcccg gagagccgcg ggagcgtcga
      841 tcgccccggc aagtttgcgg ataccgcggc aagcaccctc cttgcggaga tggagatgtg
      901 cgggagctcg aagttcgcga tcgccgcaac cctcgtcggc ggtgcgagca tgtttgagtt
      961 ctcggcgaac tccttaaaca taggcgagag aaacatcgtc gccgtgcggg atgtgctccg
     1021 cgaccagggg atacggatcg tgcgcgagga gacgggcggc aggatagggc ggtccgtcta
     1081 ctactggccg gaggggaagg gccgcatcat cgtcaagaga gcggacggaa catgcgccag
     1141 actctgatct tcttcttcat cctgtcatcg cttctcctat ccccctcgtc ggccgtctcc
     1201 gtcgacgtcc ccgactttca cgcttggatc gcatccgccg gcgtgacgga tgcgatcaac
     1261 ggccgttacg gggacgtcat atttgcgacc gacaccggca tatcgttcta cggagcgaac
     1321 ggcacctggc actcggtcaa tacacggttt ccgggggaga ccgcctacgg cctgctttcg
     1381 cccctcgact ccatggtgac cgcaatcgcc ctcgacgcgg gcggccacct ctggatagga
     1441 tacccgaacg ggctgcagat cggggatggg aaaaggtacc agagcatcca ggaccagcag
     1501 ttcctcaaga acctcaacat cgggtgcatc gtgcggtggg gagacgacat gtgggttgcc
     1561 gccggccggg cgggcctcca ccgctaccac gacggtgcct ggacgtggta taagccgctc
     1621 ggccccgaag ggctcgggtg ctacaccgtc gtgagcatgg cagtcgacgc cgcaagcgac
     1681 gcgctcgtcg tcgggtcgga acgcgacggt ctccgggttc tccggaacag aaccggggag
     1741 gtccggtttg agccggtgac ctgcgacgac gaacccgtcc gggggatatc cgaagtccgg
     1801 gtcgacccgt tcgggggcgt ctacctcttc aaccgcacga cggtccttca ctacgacccg
     1861 ggcggaaaag tcacgccaat cctcgatgcc ggcgacctga acccgttccc ggtcgccatc
     1921 aacgatctcg cggcaacctc cggggggatg cttcttatcg ctaccgatga cgggatatac
     1981 ggctggaact catcgggcgt cgccctccac atcgcctccg gcgacggcat ccgctcaaac
     2041 gtcgtcaaaa aactcttcat cgatgccgac ggccggtgct ggttcgtcgt tcccggaaac
     2101 gtgggctaca taccgccgac ggcggggcac gccaccctcg atcttgcggc cgcccccgtt
     2161 ctcgcgccgc acgaaccgga gacccctgcg atcgagactc cgacagtgga tatcccctcc
     2221 gtcgagcccg ccggggtacc ggatatgctc tcgggcgcct gggcatcctt cgtagaatgg
     2281 gtcaacgccc ggctggccgg ggcctgaggg cggcaaaccg cctcaggact tcgatatctc
     2341 cgtgtagtag tactccccgg ccgcgacctg ctcgcggtcg aggaacgacc ccggcttgtt
     2401 gatacgcggc cttccgctcc tgtcccgccg gaaagtgacg ccgagggccg agaggaatcg
     2461 gttcatgccc ggcgccatat cgaacggccc cacggccttg ccgcggatac ccggctcgcc
     2521 ctcgaagacg aggatccggt cgctgatcat atcgatgacg tagatgtcgt gatcgatgac
     2581 gagcgtgctc gcgccccggc cttcggcgtg tttgcggatc atccgcgata tcttcacccg
     2641 ctgctccacg tcgaggtggg cgctcggctc gtcgaggatg tagaggtcgg cgtcgcgcga
     2701 gaggcagacg gcgatggcca cccgctgcag ctcgccgccg gagagcgtgt tcaccggcgc
     2761 ctggaggagc ggcgagaggg agaggggttc taagatctcg tgctggtaaa acgacgtatc
     2821 gaacttcgtc gtcgtctgcc ggagaacctc ctcgacggtc gcatccgtat cgcctttgag
     2881 gtactggggt ttgtaggaga tccggacacg ggtgtccatc gacccggtat cgggctcgat
     2941 caccccggca aggagcccgg cgaacgtgct cttcccgatg ccgttcgccc cgaggacacc
     3001 gaggacctcg ccgctccgga tctctccgcc gccgacggag agagagaatt gagtaaactg
     3061 cttcgtcatc gccgggaacg tgagcaggac ctccctatcc gtcccggcgg cgtgcgcccg
     3121 ggcatcaaac gtcaccgcat acggcctgaa cctgacgttc tcttccggga ggaacccctc
     3181 gaggtactgg ttgataccga cccggacgcc cttcgggtag gtgatgacgc caaagaccgc
     3241 cggctcgccg taggcaatat gcaccgtatc ggcaagcata tcgagtatcg cgagatcgtg
     3301 ctccacgata acgaccggcc gctcccgggc gagctcccgg atcaggtctg ccgcggccat
     3361 acgctggtag acgtcgaggt agggcgtgat ctcatcgaag aagtagaagt cggcatctct
     3421 ggcaaggcat gctgcaatcg ccacccgctg cagttcgccg ccggagaggt tcgttatcgg
     3481 ccggtcgacg accgccttga gagagagccg gtcgatgtag tagccgagtt tgccgcgctc
     3541 gtcggtggtc gcgaggaggt cgcgcaccga cccggtgaag accttcggta tctggtcgat
     3601 atactgcggt tttacggcga ccttcacgtt cttctgcgag agcacctgga ggtagtcgaa
     3661 gagctcggtc ccctggtaga ggtcgaggac ctctttccag gagaccgggg cgtcggtcct
     3721 ccccaggttc gggacgagcg tgcccgagag gatcttcacc gacgtgctct tcccgatacc
     3781 gttcggtccg agtatgccgg tgaccttccc ctcgatggga atgggaaggc cgtagaggac
     3841 gaacccgttt ttgccgtagc ggtgggtcgg ctggtcgagc tcttcgggga ggttgacgat
     3901 ggcgagggcc tcgaacgggc acttcttcac gcagatgccg cacccgacgc agagttcctc
     3961 ggatatgacg gctttctggt cttcgccgat gacgacggtc tcgtcgccgg tccggacacg
     4021 cgggcagtag aggatgcatt cggtgccgca cttgacgggg tggcaccgat cgcggtgtac
     4081 aacagcgata cgcatgaggg atcacagaac ggtggtggtc agga
//
LOCUS       DAOSEE010000173         3990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_7962_length_3990_cov_228.252351, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000173 DAOSEE010000000
VERSION     DAOSEE010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7962_length_3990_cov_228.252351"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..792)
                     /locus_tag="PLI76_10200"
     CDS             complement(61..792)
                     /locus_tag="PLI76_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOI62446.1"
                     /translation="MLPSTLEDYLEAIFTIAGDGGRSVGSQEVVAALGTEEAAAGATV
                     TSLVREGYLEQLADDTVRLTEKGLAVASRVARKHRVLQCFLTEMLGVDADAADKEACT
                     LEHGISDETIDRLSSYMDSAPPGHAGRCPACEICTLERETPDEAIDRSSMSDLRRPPG
                     RARHCRGRDACTLLDCKEGDTVRVAMIRGPRRNRRLLDLGIFPGEIVQIRRKLPNHSV
                     VVRVKGCDIAISPEIAMSIIVESCP"
     gene            883..2550
                     /locus_tag="PLI76_10205"
     CDS             883..2550
                     /locus_tag="PLI76_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOI62447.1"
                     /translation="MRAPAGSVTNRDVAEQFLVMSRLLEIAGEDRYRSEAYGRASRAI
                     DRLALPVTGLDVEELVRIPGIGARIAGQIREIAETGSFDELDDMRAAVPASVVDLLGV
                     PGIGPRTLHILWKRLGILTVDDLERAVRGRRLRALRGFGPKREEAIERGIRQFRRRSN
                     RITRPQADAVLASVAALLCDGRYAVAGSYRRGASTVDGLDIVATGDRAALVRRVAAGE
                     SGDGVSFSLDGVPLNVRFTDPGRCGTALLGATGSDGFLARLGDVALARGYRFTPGGLV
                     DCSDGSLQAFASEEEVFSFLGMAFIPPELREDRGEVDLALRHALPDLIEIADIRGDLH
                     AHTTASDGRQSLEDVAEAGDARGYEYVAITDHSSRVSPEALARQQEAIERVNRRHTCE
                     LLCGSEVDIRSDGTLGYPDGVLADLDIVVASVHSGFAQDEDLLTRRILTAMENEHVDI
                     IGHPTGRLLGRRPPYAVDLARVIEHAAVTGTALEINASPLRLDLEDIYVWRAKKQGVK
                     LAAGTDAHRTSEFSNMQNAVLQARRGWCAPGDVVNTLSLSELTEWVS"
     gene            2547..3137
                     /locus_tag="PLI76_10210"
     CDS             2547..3137
                     /locus_tag="PLI76_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HOI62448.1"
                     /translation="MIYRLYEKYLLRDLSTLPQQICFMITEQDMLDAPGNLCLAAQWC
                     RDLGIGSVMFHISTADPARLARCLPLIRAVSSIGRLTLHYRDEKEVSGEGMHVTVAIG
                     KSGREEIACCVRRMAEDGIDPASVDEDLLESYLTFDYEPDLVIKTGGDHLTDFLIWQS
                     VYSELFFLDVNWALLRKVDFIRALRDFQSRARRFGR"
     gene            complement(3177..3947)
                     /gene="uppS"
                     /locus_tag="PLI76_10215"
     CDS             complement(3177..3947)
                     /gene="uppS"
                     /locus_tag="PLI76_10215"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOI62449.1"
                     /translation="MLRSGIEPLYERYLRWQVKHIPLHVAVIQDGNRRFARDQGLDRA
                     VGHRRGADTTEEFLDWACDLGVRHITLYTFSTENFRRDKDELAALFTLFQEKFAAIMT
                     DERVHRNHIRVQMIGDRSLLPDDLLATIDAAEEATLAYDDYYINIALAYGGRNEIVHA
                     ARSILDEVRQGAIDPATIGTTTVETHLHRGAPIPPVDLIIRTGNDCRTSNFLPWLANG
                     HESAVYFCAPYWPAFRRIDLLRAMRVYDQRMRLKERAA"
BASE COUNT          619 a         1258 c         1388 g          725 t
ORIGIN      
        1 cggtgagctg gttgaagatc agggatttgc cgacgctggg gttgccgatc agcgcaaact
       61 tcatggacac gactccacga tgatggacat ggctatctcg gggctgatgg cgatatcgca
      121 gcctttcacc ctgacgacga ccgaatggtt cgggagtttc cgccggatct gcacgatctc
      181 gcccgggaag atgccgaggt cgagcagcct ccggttccgc cggggccccc ggatcatcgc
      241 cacccggacg gtatcccctt ccttgcagtc gaggagcgtg caggcatcgc gcccccggca
      301 gtgccgggca cgcccgggcg ggcgccggag atcgctcatg gaggaccggt cgatcgcttc
      361 gtcgggggtc tcgcgttcga gcgtgcagat ctcgcatgcc gggcagcgcc cggcatgccc
      421 gggcggggcg ctgtccatgt aggaggagag gcggtcgatc gtctcatcgg atatgccgtg
      481 ctcgagcgtg caggcctctt tatccgctgc atccgcatcg acgccgagca tctcggtcag
      541 gaagcactgg aggacgcgat gtttccgcgc cacccgtgaa gcgaccgcga gccccttctc
      601 ggtcagcctg acggtgtcgt ccgccagttg ctccaggtat ccttccctga ccagggaggt
      661 gacggtcgcc ccggcggcgg cctcctcggt gccgagcgct gcgactacct cttgcgatcc
      721 gaccgatcgt cctccgtctc ccgcgatcgt gaagattgct tcgagataat cctcgagtgt
      781 ggaaggaagc atgatataat ctcttttact tccagcagtt tatggatttg gatggtgcca
      841 ccggtgtcaa cggctatatg gtattgcgaa cgatcggtga tcatgagggc accggcagga
      901 tcggtcacga acagggatgt tgccgaacag ttcctcgtta tgagccgcct gcttgagatt
      961 gcgggagagg accgctaccg gagcgaggcc tacgggcggg cgtcgcgggc gatcgaccgg
     1021 ctcgcccttc cggtcaccgg gctcgacgtg gaggagcttg tccgcatccc gggtataggc
     1081 gcccggatag ccgggcagat ccgggagatt gcggagaccg ggtcgtttga cgagctcgac
     1141 gatatgcggg ccgccgttcc ggcatcggtc gtcgacctgc tcggcgttcc cggcatcggg
     1201 ccgcggacgc tgcacatcct ctggaaacgg ctcgggatcc tgaccgtgga cgacctggag
     1261 cgggcggtgc ggggccgccg gctccgggcg ctcagggggt tcggcccaaa gagggaggag
     1321 gcgatcgagc gcggcatcag gcagttccgc cggaggtcga accggatcac ccggccgcag
     1381 gccgacgccg tcctcgcgag cgtggcggcg ctcctgtgcg atggtcggta tgcagttgcg
     1441 gggagttacc gccggggggc gagcaccgtc gacgggctcg atatcgtcgc caccggtgac
     1501 cgggccgccc tcgtgcgccg tgtcgcagcc ggcgagagcg gggacggggt ctcgttctct
     1561 ctcgacgggg tgcccctgaa cgtccggttc accgatcccg gccggtgcgg cacggcgctc
     1621 ctcggggcga ccggctcgga tgggttcctg gcccgcctcg gggacgtggc gcttgctcgc
     1681 ggctaccggt tcacgcccgg cggtcttgtg gattgttcgg acggcagcct gcaggcgttc
     1741 gcgagcgagg aggaggtctt ttcgttcctc ggtatggcgt tcatccctcc ggagctgcgc
     1801 gaggaccggg gcgaggtcga ccttgccctc cgccacgcgc tccccgatct tatcgagatc
     1861 gccgatatca ggggggacct gcacgcccac accaccgcaa gcgacggccg ccagtcgctt
     1921 gaggatgtgg cggaggcggg ggatgcccgg ggctacgagt acgtcgcgat caccgaccac
     1981 tcgtcacggg tgagccctga ggccctcgcc cggcagcagg aagctatcga gcgggtcaac
     2041 cggcgccaca cctgcgagct cctctgcggg agcgaggtgg acatacggag cgacggcacg
     2101 ctcggctacc ctgacggggt gcttgccgac ctcgacatcg ttgtcgcgag cgtccactcg
     2161 gggtttgccc aggacgagga tctcctgacc cggcgcatcc tcaccgcgat ggagaacgag
     2221 cacgtcgata tcatcggcca ccccaccggg cgcctcctcg ggcggcggcc gccttatgcg
     2281 gtcgaccttg cccgggtgat cgagcacgcg gccgtgacgg ggaccgcgct tgagatcaac
     2341 gcgtcgcccc tccgtctcga ccttgaggat atttatgtct ggcgtgcaaa gaaacaagga
     2401 gtgaaactgg ctgcagggac cgatgctcac cggacgagcg agttttcaaa tatgcagaac
     2461 gccgtcttgc aggcgcgccg gggctggtgc gcccctggcg acgtcgtgaa caccctctcc
     2521 ctgtcggagt tgacggagtg ggtgtcatga tctaccggct ctacgagaag tacctcctcc
     2581 gagacctcag cacgctccct caacagatct gtttcatgat caccgagcag gatatgctcg
     2641 acgcgccggg caacctctgt cttgccgccc aatggtgccg cgatctcggt atcggaagcg
     2701 tcatgttcca catcagcacg gccgatcctg cccggctggc ccggtgcctc ccgcttattc
     2761 gcgcggtctc ttcgataggg cgcctgacgc tccactaccg tgacgagaag gaggtcagcg
     2821 gggagggcat gcacgtgacg gtggcgatcg ggaagagcgg gcgggaggag atcgcctgct
     2881 gcgtccggcg gatggccgag gacggcatcg acccggcgtc ggtcgacgag gacctgctcg
     2941 agtcctacct caccttcgac tacgagcccg acctcgtcat caagacgggc ggggaccacc
     3001 tgaccgactt cctcatctgg cagtcggtct actccgagct cttcttcctc gacgtgaact
     3061 gggcgcttct ccggaaggtg gacttcatcc gggcgcttcg ggactttcag tccagggcac
     3121 gccggttcgg gcggtgatct gttccgggcg tacctcgccg gttcgggcgg cgaccgtcag
     3181 gccgcgcgct ctttcaagcg catgcgctgg tcgtagacgc gcatcgcccg caaaaggtcg
     3241 atcctcctga acgccggcca gtagggagcg cagaagtaga cggccgactc gtggccgttt
     3301 gcgagccagg ggaggaagtt cgacgtccgg cagtcgttgc cggtgcggat gatcaggtcg
     3361 acggggggga tgggggctcc ccggtggaga tgggtctcga cggtcgtggt gccgatggtg
     3421 gcggggtcga tcgcgccctg ccggacttca tcgaggatcg accgggcggc gtgcacgatc
     3481 tcgtttcggc ccccgtaggc gagggcgatg ttgatgtagt agtcgtcgta ggcgagcgtc
     3541 gcctcctcgg cggcgtcgat ggttgcaagc aggtcgtcgg ggaggaggga gcggtccccg
     3601 atcatctgta cccgtatgtg gttgcggtgc acccgctcgt cggtcatgat ggcggcgaac
     3661 ttctcctgga agagggtgaa gagggctgcc agctcgtcct tatcccgccg gaagttctcg
     3721 gtcgagaagg tgtagagcgt tatgtgccgg acgccgaggt cgcatgccca gtcgaggaac
     3781 tcctccgtgg tatctgcacc gcgccggtgc ccgaccgccc tgtccagccc ctggtcccgg
     3841 gcaaaccggc ggttgccgtc ctggatcacc gcgacgtgaa gggggatgtg tttcacctgc
     3901 catcgcaggt agcgttcata gagaggctca atcccggatc gcagcatcga gcggggtcac
     3961 ctccacgacc atgccgccgt ttgggtagcg
//
LOCUS       DAOSEE010000174         3981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_7988_length_3981_cov_247.178553, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000174 DAOSEE010000000
VERSION     DAOSEE010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7988_length_3981_cov_247.178553"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..456
                     /locus_tag="PLI76_10220"
     CDS             31..456
                     /locus_tag="PLI76_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HOI62450.1"
                     /translation="MILSSSEIARRREDGDLVIEPYHPASQQPASYDLRAAGATVLPC
                     GACTLVPSIERVELPADLAATLRCRSSLARRGVLIGGGFVDPGFRGQLTLCLTNMGPE
                     PITLAGGDRVVQMVVQEVRNGDRLYRGRYQDSLGAVHTR"
     gene            453..1421
                     /locus_tag="PLI76_10225"
     CDS             453..1421
                     /locus_tag="PLI76_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrpR regulatory domain-containing protein"
                     /protein_id="HOI62451.1"
                     /translation="MSPSIHSERRYLELLRILAESHEPLGAKRLSEKMAERGFVLSDR
                     AVQYYLQYLDEMGFTEKVGNRGRILTEAGTAESESALVDERIGFIISRLEQLAFRSTF
                     DPSTGTGSVAYNLSLVREEDLPGVAAAFDEVAEAGYGFLGTYRVVDADPRIPDGHVGL
                     MTVCSVTLDGLLQKRGIPARLEYAGRMIVGENGTAGFLDLIGYRGTSVDPLHLFISAG
                     LTAINRLVTTRTGIALANIRMVPAAARDRVKEAADLMMECGFTFPAGNGIGEFNLPGH
                     PYRLSVVAFSGMNMVANAVEKGYVVRTEIGAGTIPFERMADTTGSR"
     gene            complement(1405..3330)
                     /locus_tag="PLI76_10230"
     CDS             complement(1405..3330)
                     /locus_tag="PLI76_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tubulin/FtsZ family protein"
                     /protein_id="HOI62452.1"
                     /translation="MRAFPRVNGHPGHAKVRGYNDMRVLAIGLGSAGSRVVDNLYDHD
                     RRSRVCCMSAVAIDIDPNSLIQLRHLPDPARVFFPRIDPADQGGVASVIDIEEVMTRI
                     QGMDTMEIDAILLCCGLGGNVADIAPLVIGELRTSYMEPIFALAILPCLNEGKRVSAK
                     AADDLDALQALVDAVILFDNETWSQRITAAATAAGNTGIVDQLRRNAPSGELDPRTRY
                     NLLNERIARQIGLLLRAGEFNESGLEVAEVVLDAGEVLNTLKGNGLVAVGYAAERLPT
                     GWLDRIYRRRSLKFFIQGSQEKAARIVSLAKKAVYEEISVPCDLTSADKALVLIAGPS
                     TELSMKGFQTVRKWIDRNIAGLEMRSGDYPVKNTSFVGIIIVLSGLANVPRVEELREI
                     RAEYRLECEEEALGAKEEAAREPVVAPAMREEDIPEFASPIEAAFLKESDPMAEESTA
                     EKDEMIVLTGGGGNNRKSRDETIDMLPRAVKSQDDGAVILPPKEGGREIDLAGSASVT
                     SSVPAPKNATFGLKGIRIGKTAPKDDDTLFAASLKPVHRPKDDVLTGDTVVLDRGVRR
                     PRDGTLDEPGLHMRGAAPAPKDAPLDQTGRGFGRGGPHPKEVDPSRGRLDVIDAAERP
                     KKRPEDDRGDDGITWIR"
     gene            3606..>3981
                     /locus_tag="PLI76_10235"
     CDS             3606..>3981
                     /locus_tag="PLI76_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HOI62453.1"
                     /translation="MRCIALTTYGIEFVPGAINVKQVVTYTKLAESKDIDYAWITNHY
                     NNRHAYPTLAMIAANTDTIKMGPGIMNTFTDTPAAIASFMATLNEISDGRAVLGIGPG
                     DLSTLPKLAIDPVKPVGHLKEGV"
BASE COUNT          710 a         1322 c         1186 g          763 t
ORIGIN      
        1 ttaagcgttt ttatgccagt acactatacg atgatcctct catccagcga aatcgctcgc
       61 cggcgcgagg acggcgatct cgtcatcgaa ccgtaccacc ccgcatccca gcagcccgca
      121 tcgtacgacc tccgcgcggc cggggcaacc gtgctcccgt gcggcgcatg cacccttgtc
      181 ccctcgatcg agcgggtgga actccccgcc gatctcgcgg caaccctccg gtgccgctcc
      241 tcgcttgccc gccgtggcgt cctcatcggc ggcgggttcg tcgatcccgg tttccgtggc
      301 cagctgaccc tctgcctgac caacatgggc ccggaaccca taaccctcgc cggaggggac
      361 cgggtcgtcc agatggtcgt ccaggaagtc aggaacggcg accggctcta ccggggccgg
      421 taccaggaca gccttggcgc ggtgcatacg cgatgagccc atccatacat tccgagcgga
      481 gatacctcga actcctccgg atcctcgcgg aatcgcacga acccctgggt gcgaagaggt
      541 taagcgagaa gatggccgaa cgggggttcg tcctgagcga ccgcgccgtc cagtactacc
      601 tccagtacct cgacgagatg gggttcacgg agaaggtcgg aaaccggggg cgtatcctca
      661 ccgaggcagg gaccgccgag agcgagagcg ctctcgtcga cgaacggatc ggtttcatca
      721 tatcgaggct cgaacagctc gccttccgga gcaccttcga cccgtcgacc gggaccggga
      781 gcgtcgccta caacctctcc ctcgtgcggg aggaggacct cccgggcgtt gcggcggcct
      841 tcgacgaggt ggcggaggcc ggctacgggt tcctcggcac ctaccgggtc gtcgatgccg
      901 acccccggat acccgacggc cacgtcgggc tcatgacggt ctgcagcgtc accctggatg
      961 gtctcctcca gaagaggggg atacccgcga ggcttgagta cgcgggaagg atgattgtcg
     1021 gcgagaacgg caccgccggg tttcttgacc tcatcggcta ccgggggacg tcggtcgacc
     1081 cgctccacct cttcatatcc gcgggcctca ccgcgatcaa ccggctggtg acgacccgga
     1141 ccggtatcgc gcttgcaaac atccgtatgg tcccggcggc cgcgcgggac cgggtaaaag
     1201 aggctgccga cctgatgatg gagtgcgggt tcaccttccc cgccgggaac gggatcgggg
     1261 agttcaacct ccccgggcac ccctaccggc tctccgtcgt cgccttcagc ggcatgaaca
     1321 tggtggcaaa cgccgttgag aaaggctacg tcgtcaggac cgagatcggc gccggaacca
     1381 taccgtttga gcggatggcg gatactaccg gatccaggtg atcccgtcgt cgccgcggtc
     1441 gtcttccggt cttttcttcg gccgttcggc cgcatcgatc acgtcgagcc ttccgcggga
     1501 gggatccacc tcctttgggt ggggtccccc gcggccgaac ccccggccgg tctggtccag
     1561 cggggcatcc ttcggcgcgg gagccgcccc gcgcatatgc aggccgggtt catcgagggt
     1621 cccgtcgcgc ggccgccgca ccccgcggtc cagcacgacc gtatcgccgg tgagcacatc
     1681 gtctttcggg cggtgcacag gttttaagga tgcggcgaag agcgtatcgt catcctttgg
     1741 agccgtcttc ccgatgcgga tccccttcag cccgaaggtg gcgttcttcg gcgcaggcac
     1801 ggacgatgtg accgatgccg acccggcaag gtcgatctcc ctcccgccct ctttcggggg
     1861 aaggatgacg gcgccgtcgt cctgactctt cacggctctc gggagcatat cgatggtctc
     1921 gtccctgctc tttctgttgt tgccaccgcc gcccgtaagc acgatcatct cgtccttttc
     1981 tgctgtagac tcttctgcca tagggtcact ctccttgaga aatgccgcct cgatgggcga
     2041 cgcgaactcc gggatatctt cctcccgcat cgccggggcg accaccggct ccctggccgc
     2101 ctcctctttt gctcccagcg cctcctcctc gcactcgaga cgatactcgg cccggatctc
     2161 cctgagttcc tcgacgcggg ggacgtttgc gagccccgag agcacgatga tgatcccgac
     2221 aaacgatgtg ttcttcaccg ggtagtcgcc ggaacgcatc tcgaggccgg cgatgttccg
     2281 gtcgatccac ttcctgaccg tctgaaaccc cttcatcgag agctcggtag aaggccctgc
     2341 gatcaggacg agggccttgt cggcgctcgt gaggtcgcag gggaccgata tctcctcgta
     2401 aaccgctttc tttgcgagcg agacgatccg ggccgctttc tcctgagagc cctggatgaa
     2461 gaacttcagc gaccgccgcc ggtagatgcg gtccagccac ccggtcggca ggcgttcggc
     2521 agcgtagccg accgcaacga gaccgttccc tttcagggtg ttgagcacct ctccggcgtc
     2581 caggacgacc tcggcgacct cgagcccgga ctcgttgaac tcgccggcgc ggaggaggag
     2641 gccgatctgc cgggcaatcc gctcgttgag caggttgtag cgggtccgcg gatcgagttc
     2701 tcctgaaggt gcatttctgc gcagctggtc cacgatcccg gtgttcccgg ctgcggtcgc
     2761 ggccgccgtg atcctctgcg accaggtctc gttatcgaag agaatgacgg catcgacaag
     2821 cgcctgaagt gcgtcgaggt cgtcggcggc ttttgccgag acccgctttc cctcgttgag
     2881 gcacggcagg atggcaaggg cgaatatcgg ttccatgtaa gacgtccgga gctcgccgat
     2941 gacgagcggt gcgatatcgg cgacgttgcc cccgagcccg cagcagagca ggatggcatc
     3001 gatctccatc gtatccatgc cctggatgcg ggtcatgacc tcctcgatgt cgatcacgct
     3061 cgcgaccccg ccctggtccg ccggatcgat ccgcgggaag aagacccttg ccgggtccgg
     3121 gagatggcgg agctggatca gcgaattcgg gtcgatatcg atcgccaccg cactcatgca
     3181 gcagactctg ctccgcctgt cgtggtcata gaggttgtcc acaactcgcg accccgcgct
     3241 cccgagtccg atcgccagca cccgcatatc gttataccct cgaaccttcg catgcccggg
     3301 atgaccgttg acgcgaggaa aagcgcgcat atcatccgga actactgatg agtatgtggg
     3361 cagacaaact tatatatttc tgtacgtccc cgggatcccg gagcgcatac gttcacaaac
     3421 gttctatatt tgaacattcc ggttctcttc ccgtgtatct gcgccgcctg cagggtaagg
     3481 gagaggattc atcggatatc cttataaacc tgttacatcc ctctgttttg ccttgcatgt
     3541 gcggtacacc tcgtttcacg agcacaaaat ggtaatatat ataactccct gcacgtaact
     3601 actcaatgag gtgcatagcc ttgactacat atggaattga atttgtgccc ggagcaatca
     3661 acgtcaagca ggtggtgacc tacaccaagc tcgcagagtc gaaggatatc gactatgcct
     3721 ggatcaccaa ccactacaac aaccgtcatg catacccgac cctcgccatg atcgcggcga
     3781 ataccgacac gatcaagatg ggaccgggca tcatgaacac gttcaccgac accccggcag
     3841 ccatcgcgtc cttcatggct accttaaacg agatctccga cggacgtgcc gtcctcggca
     3901 tcggtcccgg cgacctctcg acgcttccga agctcgccat cgaccccgtc aagcccgtcg
     3961 gccacctgaa ggaaggcgtc g
//
LOCUS       DAOSEE010000175         3925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_8136_length_3925_cov_324.148062, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000175 DAOSEE010000000
VERSION     DAOSEE010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8136_length_3925_cov_324.148062"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..607)
                     /locus_tag="PLI76_10240"
     CDS             complement(194..607)
                     /locus_tag="PLI76_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOI62454.1"
                     /translation="MFRTTIRVETHGEGEIVDLTPQIAQAVAESGVREGLANIFVAGS
                     TAAVTTIEYEPGVLSDLSRALAVIAPADIPYAHDAAWGDGNGRSHVRAAIVGPSLSVP
                     VIAGALACGTWQQIVLLELDVRSRRERSVYVTVVE"
     gene            complement(895..1119)
                     /locus_tag="PLI76_10245"
     CDS             complement(895..1119)
                     /locus_tag="PLI76_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOI62455.1"
                     /translation="MKNRIKVFRAMHDMTQEELADTIQVTRRTINSIERGKYNPSIEV
                     AYKIAKTFGVTIEEVFCFEDNDGEDTEAGR"
     gene            complement(1125..1727)
                     /locus_tag="PLI76_10250"
     CDS             complement(1125..1727)
                     /locus_tag="PLI76_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2178 domain-containing protein"
                     /protein_id="HOI62456.1"
                     /translation="MKQRTYRICIALVAIALAFALLLGLAFANVIIPVIAVVVGIAIL
                     ALCRRRVTEVTEDELSTILHGKAALGALEITILVAAIGFAALMSFSSGGGSGWGLATY
                     DNGSTKVYYGVLSISPILSVSPLDLNYESYSLYEGSYLIPDPANLTFDDVVALDAMFA
                     EGHRIRDTARAFGAALGMVTVLLAVLYGAFLWYYNRKYGA"
     gene            complement(1896..>3925)
                     /gene="gyrA"
                     /locus_tag="PLI76_10255"
     CDS             complement(1896..>3925)
                     /gene="gyrA"
                     /locus_tag="PLI76_10255"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOI62457.1"
                     /translation="IEKNTVDFVPNFDESLKEPDVLPARVPNLLVNGSSGIAVGMATN
                     MPPHNLREVCEATCRIIDEPEISTEELMQIMPGPDFPTGGIMMGTGGVRDAYLTGRGK
                     CVVRGVAEIEEEGRTPQIIITEIPFQVNKARLIENIASLVRDKRIEGISDIRDESDRD
                     GMRVVIDLKKGVAPQVILNYLYKHTALESSFGIINLAIVDRQPKVLNLKELVHEFLKH
                     RVNVVRRRTEFDLAKTEERRHILSGLLVALDNIDAVIATIRASGTTEEALKALVAKFG
                     LDEAQADAILKMQLRRLAALEHQKILDERDALVKEVERLSAILASEASILAEIKKELI
                     DISARYGDERRTRIGYAAEAFDREDLIEDKPMLVSLTTSNYIKRIDLDTYRNQRRGGR
                     GVIGMATKDNDGVENVFVANMHDNLLCFTDRGRVYWLKVYDLPEGQRTGKGKALVNLL
                     NLDGEERVTAVIPVREFRPDRYLFFATRGGTVAKMALDEFSRPRQTGINAINLRDDDE
                     LVDVKATDGNQELILTTKFGQSLRFHEETVRPVHRNAMGVRGIKLRNGDTLQAISVVE
                     EDHLLTITEQGFGKRTEFDEFRGHGRGTMGVRNIVVDARGGGVVGSRAVSDDDEIIVM
                     SASGIVIRTKVSEISVQKRGTRGVRIMRLDDGDRVIGFTILDSEEEDGEEA"
BASE COUNT          698 a         1212 c         1212 g          803 t
ORIGIN      
        1 cggtccacgc ccggacccgg atctgggaac tggtatcgca gctggagacg gtaccactcg
       61 attaaatcac tcattcttcc tgaactattc cggcgaagac cagggcagag cgatacgtat
      121 tctcgggttg cccgtcaggc gaccctatcg ctctgcccgc agtatcgccg ccgcgcgtct
      181 ccttcctcgt gtttcattct acgacggtga cgtaaaccga acgctcgcgt ctcgaccgca
      241 cgtcgagctc caagaggacg atctgctgcc aggtgccgca cgcgagcgca ccggcgatca
      301 ccgggacgga gagcgacggg ccgacaatcg ccgccctgac gtgagaccgc ccgttgccgt
      361 cgccccatgc ggcatcgtgc gcgtacggga tatcggccgg ggcgatgacc gcgagcgccc
      421 gggaaaggtc cgagagcaca ccgggctcgt actcgatggt ggtgacggcg gcggtcgacc
      481 ctgcaacgaa gatgttcgca aggccctccc gcacgccgct ctcggcgacc gcctgcgcta
      541 tttggggcgt gaggtcgacg atttccccct ccccgtgggt ctctacccgg attgtcgtac
      601 gaaacatcat ggacacctca tccacacgcc ctgttgcctc ctggctggat aaaactcgcg
      661 gaacgggcgg tggcacgggg tgccgggatc tggcacgtta cggggacggt tcccggtgca
      721 gaaaggctcc ccggggcgtg ctcgtcgtca acgctttata gatgaggcag atatttcccc
      781 tccggagatg gtggttatgc cctaacgatg gaacgacaga caggatatgg agggggcggt
      841 cgtcgttcac cgccggaacg ccgttgcaga ccccggtaag atagtcgcaa cggatcatcg
      901 cccggcctcc gtatcctcgc cgtcattgtc ctcgaagcag aagacctcct cgatggtgac
      961 gccgaaggtc ttcgcgatct tgtaagctac ctcgatggag gggttgtact tgccccgctc
     1021 gatggagttg atcgtccgcc gggtgacctg gatcgtatcg gcaagttcct cctgggtcat
     1081 gtcgtgcatc gcgcgaaaga ccttgatcct gttcttcatc ggttctatgc cccgtatttc
     1141 ctgttgtaat accacaaaaa cgcgccgtac agcacggcca gcagtaccgt gaccatcccg
     1201 agtgctgcgc cgaacgcgcg ggccgtatcc cggatgcgat ggccttctgc aaacatcgca
     1261 tcaagggcga cgacatcgtc aaaagtcagg ttcgccgggt cggggatgag gtaggagcct
     1321 tcgtacaggg agtaactttc gtaattcaga tctagaggag agacggagag tattggagag
     1381 atagagagta ccccgtagta gaccttggtc gacccattgt cgtatgttgc caggccccaa
     1441 ccggatccgc cgccggacga aaagctcatc agcgcggcaa acccgatcgc ggcgacgagg
     1501 atggttatct cgagtgcgcc gagcgccgcc ttcccgtgca ggatcgtcga gagctcgtcc
     1561 tccgtgacct ccgtcaccct gcggcggcag agggcgagga tggcgatacc gacgacgacg
     1621 gcgatgaccg ggatgatcac gtttgcgaat gccaggccga gcaagagagc aaaggccagg
     1681 gcgattgcca ccagagcgat gcagatgcga taggttcgtt gcttcatgct tccactatgt
     1741 aacgcgtaat tctcttattg tgtaaataac ttctcatatg gtgcgacacg cttctctatg
     1801 gggtcggcat gtcccgggca tcaaaacagt caattagagg atgaaggctg cggagaaatc
     1861 cagaaaaaga gtgtaaaaag agagggggcg ggcgcttacg cctcttcccc atcttcttct
     1921 tcggagtcca ggatcgtgaa cccgatgacg cggtcgccgt cgtcgagccg catgatacgg
     1981 acaccacggg tgccccgctt ctggaccgag atctcggaaa ctttcgtcct gatcacgatg
     2041 ccggacgcac tcataacgat gatctcgtcg tcgtcggaga ctgctctcga cccgacgaca
     2101 ccgccgcccc gggcgtcgac gacgatgttt cgcacgccca tcgtaccccg gccgtggccg
     2161 cggaactcgt cgaactccgt ccgcttcccg aagccctgct ccgtgatggt gaggaggtgg
     2221 tcctcctcga ccacggatat ggcctgcagg gtatcgccgt tgcggagctt gatcccccgc
     2281 acgcccatgg cgttgcggtg aaccgggcga acggtctcct cgtggaaccg gaggctctgc
     2341 ccgaacttcg tcgtcaggat cagttcctgg tttccgtcgg tcgccttcac gtccacgagc
     2401 tcgtcatcgt cgcggaggtt gatggcgttg atccccgtct gccggggccg cgagaactcg
     2461 tcgagcgcca tcttcgcgac cgttcctcct ctcgtcgcaa agaagaggta gcggtccggc
     2521 cggaactccc tgaccgggat caccgcggtc acccgctctt cgccgtcgag gttcaggaga
     2581 ttgacgaggg ccttgccctt gcccgtccgc tgcccctcgg ggaggtcgta gaccttcagc
     2641 cagtaaacgc gtccccggtc ggtgaagcag aggaggttgt cgtgcatatt cgcgacgaag
     2701 acgttctcga cgccatcatt gtcctttgtg gccatgccga tgacgccgcg gcctccgcgc
     2761 cgctggttgc ggtaggtgtc gaggtcaatc cttttgatgt agttcgaggt ggtgagcgag
     2821 accagcatcg gtttatcctc gatgaggtcc tccctatcga aggcctcggc tgcgtacccg
     2881 atccgggtcc tccgctcatc accgtagcgg gcactgatgt cgatgagttc ctttttgatc
     2941 tcggcgagga tgctcgcctc gcttgcgagg atagcagaga gccgctcaac ctcctttacg
     3001 agggcatcgc gctcgtccag gatcttctgg tgctcgagcg ccgcaagccg ccggagctgc
     3061 atcttcaata tggcgtccgc ctgcgcctcg tccagcccga acttcgccac cagggccttc
     3121 agtgcctcct cggtcgtccc ggatgcccgg atggttgcga tgacggcgtc gatgttgtcg
     3181 agcgcaacaa ggagaccgct gaggatgtgc ctccgctcct ccgtctttgc gagatcgaac
     3241 tcggtccgcc gccggaccac gttcacccgg tgcttgagga actcgtggac gagctccttg
     3301 aggttcagga ccttgggctg gcggtcgacg atggcgaggt tgatgatgcc gaaggaggac
     3361 tcgagcgccg tgtgcttgta gaggtagttg agtatcacct ggggcgcgac gcccttcttc
     3421 aggtcgatga cgacccgcat gccgtcccgg tccgactcgt cgcggatgtc ggagatgccc
     3481 tcgatccttt tgtcgcggac gaggcttgcg atattctcga tcaggcgtgc cttgttcacc
     3541 tggaagggga tttcggtgat gatgatctgc ggagtcctgc cttcctcctc gatctccgcg
     3601 accccccgga caacacactt cccgcgcccg gtgaggtagg cgtcccggac tcctccggtg
     3661 cccatcatga tcccgccggt cgggaagtcc gggccgggca tgatctgcat cagttcctcc
     3721 gtggagatct caggctcgtc gatgatgcgg caggtcgcct cgcagacctc ccggaggttg
     3781 tggggcggca tgtttgtcgc catgccgacc gcgatcccgc tcgacccgtt gacgagaaga
     3841 tttgggaccc gggccgggag gacgtcgggt tccttgagcg actcatcgaa gttggggacg
     3901 aagtcgacgg tattcttctc gatat
//
LOCUS       DAOSEE010000176         3920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_8158_length_3920_cov_231.412160, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000176 DAOSEE010000000
VERSION     DAOSEE010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8158_length_3920_cov_231.412160"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PLI76_10260"
     CDS             <1..369
                     /locus_tag="PLI76_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOI62458.1"
                     /translation="SAVQVCTAVMWEGAGIIRAMNAGLSGYLAEKRFPSVGALRGRAL
                     SRIGTHAALSRSVHRIAIRQFPERCASCGRCVTACRDGGYRAISIADRQVAIDAERCD
                     GCSLCSYVCPEGVIVMLPGA"
     gene            complement(558..1772)
                     /locus_tag="PLI76_10265"
     CDS             complement(558..1772)
                     /locus_tag="PLI76_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HOI62459.1"
                     /translation="MKKLSLFIALLLVIPAAALALSSGSMESAPTGSGLTQVATFENG
                     TRYQAGAYPVVVLSGTYREMGRQYGALMKDDLTANYAFVYASFTDRGYNVSQMNEFAR
                     TGTDLQPKRMKEIREGMAETSGLTVEELEILYMEVCVVMSAVLQTEPACSFMAVWGNY
                     TNDGSVVLSRNYDMPDVISPYYPYYTLVVYNPTDGSNGVATFNPVGARTETLMNSAGL
                     YISENNGAHAVGTMEVANRPDLIDEFFGMMLDYSDMKGLDAALMAARSDYAWIVNTAC
                     PDFAYSYEESVYDIKRLEGHEIIVAANHFDDPTWYIEGPPVVNSITRCHNLRRQAEEN
                     RGSIDGERMVEIRNVLMRNGGATFLHDDVSGDMTYSTEYQVVYSPETRTLWMKVVDRD
                     WQKVELGPLFAV"
     gene            complement(1978..3216)
                     /locus_tag="PLI76_10270"
     CDS             complement(1978..3216)
                     /locus_tag="PLI76_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-activating nucleotidase"
                     /protein_id="HOI62460.1"
                     /translation="MGDIARQAPDKDTGEDIYQYLLERITNLENRNLELREQFRQMES
                     EKRYVETQKIRYERELRKLKSEIEQLRSPPLVIGTVTDVIDNNRVIVRSSAGPRFLVR
                     TSQLIDPDLLKPGVRCTLNQQSLAIVDVLPTSYDAQIYGMELVESPEETYENIGGLEA
                     QIEEVREAVELPLTKPHLFEKVGISPPKGVLLHGPPGTGKTLLARAVAHQTNAHFLRV
                     VGSELVQKYIGEGARLVRELFELAKQKSPSIIFIDEIDAIGAHRSDSTTSGDREVQRT
                     LMQLLAEMDGFDNRGDVKIIAATNRIDILDRALLRPGRFDRMIEIPLPDHTGRLAILK
                     IHTQSMNLGEDVNLPDVARLTEGKNGADLRAICMEAGMFAIRMERDTVSADDFMKAIE
                     KLSVDFDRHHFHTTFGEMFA"
     gene            3274..3762
                     /locus_tag="PLI76_10275"
     CDS             3274..3762
                     /locus_tag="PLI76_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein bridging factor aMBF1"
                     /protein_id="HOI62461.1"
                     /translation="MQCELCGALIQGPPKTIQIEGAELCVCVRCAKHGTEVQQTRRKG
                     APQQKSGVAVPQARRKPRDVFDLMEGEIVDDYADRIRVAREGKEWSTLDLAQAIKERE
                     ILVKKIEKGDLIPEDDVRRKLEKALNIRLIDSAEDSASAGGPGRVTMTVGDVISFKKS
                     RK"
BASE COUNT          774 a         1181 c         1161 g          804 t
ORIGIN      
        1 tcggcggtcc aggtctgcac ggcggtgatg tgggaggggg cggggatcat ccgcgcaatg
       61 aatgcgggcc tgtcgggata ccttgcagaa aaacgctttc cgagcgtcgg ggccctcagg
      121 ggccgcgccc tatcccgcat cgggacgcat gccgccctct ccaggagcgt tcaccgcatc
      181 gcaatcaggc aatttcccga acgatgtgca tcctgcggcc gctgcgtgac ggcctgccgc
      241 gacgggggat accgtgccat atcgatagcc gaccggcagg tcgcgataga tgcagaacgg
      301 tgcgacgggt gcagtctctg ctcgtatgta tgtccggaag gtgtgatcgt catgctgccc
      361 ggagcgtgaa cgggtacggc tcgcccgcgg cattgccgcc gcaccggatc ccggtccccc
      421 tccccgtcag ggtcgcacga gtgtcggata ggacggaaac gtccatatcc ggtattacaa
      481 ccggaccaag aagttcagtc cggacggcac gattaacctg tttttgaaaa aaaataaccc
      541 ggagtttccg ttgccggtta caccgcaaac aacggcccga gctccacttt ttgccagtcc
      601 ctatccacga ccttcatcca gagcgtccgc gtctccgggc tatagaccac ctgatactcc
      661 gtcgagtagg tcatgtctcc cgatacatcg tcgtgcagaa aggtcgctcc accgttacgc
      721 atcagaacgt tccggatctc gaccatacgc tctccgtcta tcgagcccct gttctcttcc
      781 gcctgacgcc ggaggttgtg gcaccgcgtg atggagttta ccaccggagg cccttcgata
      841 taccatgtcg ggtcgtcgaa gtggtttgcg gcgacgatga tctcgtgccc ttcgagccgc
      901 ttgatgtcgt atacgctctc ctcataggag tatgcgaagt ccgggcaggc cgtgttcacg
      961 atccacgcat aatccgaccg ggcggccatg agggcggcat cgagcccctt catatccgag
     1021 tagtcgagca tcatcccgaa gaactcgtcg atcaggtccg gcctgttcgc gacctccatg
     1081 gtgccgacgg catgtgcacc gttgttctcc gagatgtaga gcccggcgct gttcatcaat
     1141 gtctcggtac gagcccctac agggttgaag gttgccacac cgttgctccc gtcggtcgga
     1201 ttgtagacga cgagggtata gtacgggtag tagggcgata tcacgtcggg catatcatag
     1261 ttccgggaga gaaccaccga accgtcattc gtgtagttgc cccacacggc catgaacgaa
     1321 catgcaggct ccgtctgcaa gactgcggac ataacgacgc acacctccat gtagaggatc
     1381 tccagttcct ccaccgtcag gccggaggtc tccgccatgc cctcccggat ctccttcatg
     1441 cgctttggct gaagatccgt ccccgtccgt gcaaattcgt tcatctgcga gacattgtag
     1501 cctcggtcgg tgaaggaggc gtatacgaat gcataattgg cagtgagatc gtccttcatc
     1561 agcgccccat attgccggcc catctcccgg taggtgccgg agaggacgac gaccgggtac
     1621 gcccccgcct ggtagcgcgt accgttctcg aacgtagcca cctgcgtaag cccgctcccc
     1681 gttggggccg attccatgct cccggaggag agagcgaggg ctgccgccgg gataacaagc
     1741 aggagtgcaa tgaaaaggga gagtttcttc atacagagca gtgcatgccc aaacgataaa
     1801 aaagagcatg atttatctct tccctcccgc gcatagactc caataatcaa cgcgataccg
     1861 attccggccc cgcagatcgg ccttgccggg ccacacgacc ccctccgttc ggagagaaac
     1921 atcgtcgggg catcctcacg ggagttcgaa aaaaagtgcg taaaaagaaa acgcccttca
     1981 ggcgaacatc tcgccgaagg tggtatggaa gtggtggcgg tcgaagtcca ccgagagttt
     2041 ctcgatcgcc ttcatgaaat cgtcggcgct gaccgtatcg cgctccatcc tgatggcgaa
     2101 catccccgcc tccatgcaga tcgcccggag gtcggcgccg ttcttcccct cggtgagccg
     2161 ggccacgtcc gggaggttga cgtcctcgcc gaggttcatg ctctgggtgt ggatcttcaa
     2221 gatggcaagc cttccggtgt ggtcggggag cggtatctcg atcatccggt cgaaccggcc
     2281 ggggcggaga agggcgcggt cgagtatgtc aatccggttg gtcgccgcga tgatcttgac
     2341 gtccccgcgg ttgtcaaacc catccatctc ggccaggagc tgcatcagcg tccgctggac
     2401 ctcgcggtcc cctgaggtcg tcgagtcgct ccggtgcgcg cctatcgcat cgatctcgtc
     2461 gatgaatatg atggagggtg acttctgctt cgcaagctcg aagagctccc tgaccaggcg
     2521 ggccccctcc ccgatgtact tctgcacgag ctccgagccg accacgcgga ggaaatgcgc
     2581 attcgtctgg tgcgccaccg cccgcgcgag gagcgtcttc cccgtgccgg gcgggccgtg
     2641 gaggaggacc cccttcggcg gggagatgcc gaccttctcg aagaggtgcg gctttgtcag
     2701 ggggagctcg accgcttccc tgacctcctc gatctgcgct tcaaggccgc cgatgttctc
     2761 gtaggtctcc tccggcgact ccaccagttc catgccgtag atctgcgcgt cgtagctcgt
     2821 ggggagcaca tcgacgatgg cgagggactg ctggttgagt gtgcaccgca cgcccggttt
     2881 gaggagatcg gggtcgataa gctgcgatgt acggaccagg aaccgcggcc ctgcgctgct
     2941 tcgtacaatc accctgttgt tgtcgataac gtcggtaacc gttccgatca caagaggagg
     3001 gctcctgagc tgctcgatct cgctcttgag ctttcgcagc tcccgctcgt agcggatctt
     3061 ctgagtctcg acatatcgtt tttcggactc catctggcgg aattgttcgc gtagctccag
     3121 gtttcggttt tcaaggttcg ttatccgttc caggagatac tggtagatgt cttcaccggt
     3181 atccttgtcg ggtgcctgcc gagcgatatc tcccataggt ctcctcgatt aattatatag
     3241 gttaaaatga atataaagtt gtttgcgaga cttatgcagt gcgaactatg cggcgctctc
     3301 atacaggggc caccgaaaac catccagatc gagggtgcag agctctgcgt ctgcgtccga
     3361 tgcgcaaaac acggtacaga agtccagcaa acgcgccgga aaggcgcccc gcaacagaaa
     3421 tcgggggtcg ccgtcccaca ggcccggagg aagccgcggg acgtctttga cctcatggaa
     3481 ggggagatcg tcgacgatta cgccgatcga atccgggtag cccgcgaagg gaaggagtgg
     3541 tccaccctcg atcttgccca ggcgatcaag gaacgcgaga tcctggtcaa gaagatagag
     3601 aagggagacc tcatccccga agacgacgtc cggcggaaac tggagaaagc gctcaatatc
     3661 cggctgatcg actcggccga agacagcgcc tccgccggcg ggccgggccg ggtgaccatg
     3721 accgtcggtg acgtcatatc gttcaaaaag agccgcaaat aaggtctacc ccatactttc
     3781 tctcacgagg agcggccttg ccccggatcc gcgctgtgcg cggtattccg gcatattttt
     3841 atacgaggaa acgtaacgat atacgcaatg cgtggtgaat gttttttcgc cggtgaccgc
     3901 ttttacctcg gttaacggag
//
LOCUS       DAOSEE010000177         3897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_8221_length_3897_cov_174.939094, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000177 DAOSEE010000000
VERSION     DAOSEE010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8221_length_3897_cov_174.939094"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            421..723
                     /locus_tag="PLI76_10280"
     CDS             421..723
                     /locus_tag="PLI76_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOI62462.1"
                     /translation="MSPGTDIYTLLIQRRDEILTLAHRRGAKNVRVFGSVARKEARED
                     SDIDILIDLDPDRSLLDVGGLAMDLSLLLDRPVDVVTEAGLRERIRSRVVREARAL"
     gene            837..1052
                     /locus_tag="PLI76_10285"
     CDS             837..1052
                     /locus_tag="PLI76_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOI62463.1"
                     /translation="MIHHLLIIGEAVRGISLEFRTANPDIPWSDIVGMRNVLVHHYFG
                     IDRNAVWNVVEHDLPALKEQLLPLIQE"
     gene            1155..1391
                     /locus_tag="PLI76_10290"
     CDS             1155..1391
                     /locus_tag="PLI76_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62464.1"
                     /translation="MSATTVWITPANKDRLEGLKRHPRESYNDVIGRLLDMTVDDEPL
                     SEEAIRGIEEALEDIKAGRLYSEDDIRKEFGVEE"
     gene            1388..1648
                     /locus_tag="PLI76_10295"
     CDS             1388..1648
                     /locus_tag="PLI76_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOI62465.1"
                     /translation="MTYTVTYTARARRDLQNLPPEAARRIIRSVAGIRENPYRHVRKL
                     AGFSRTPVYRLRIDRYRVILTIEDERLLILVLEAGDRSSIYR"
     gene            2274..2732
                     /locus_tag="PLI76_10300"
     CDS             2274..2732
                     /locus_tag="PLI76_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin N-terminal domain-containing
                     protein"
                     /protein_id="HOI62466.1"
                     /translation="MMKFRENEDAVSPVIGVILMVAITVILAAVIAAFVFGMTGNVQT
                     TKTVAVTANQNSNNVVLTYHGGADAATLQYLTITVTKGSDTTSETQYLWYDTSSSGVV
                     TTNPNENNVGKLPVGTTTTIVGFASSQQDHVVVAGHFADGSDHVVLDTYV"
     gene            2760..>3897
                     /locus_tag="PLI76_10305"
     CDS             2760..>3897
                     /locus_tag="PLI76_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62467.1"
                     /translation="MKKILFITFICCLLSLAIGYPLLYLTDEWITADQLNHLITGDDL
                     LFGYEPYGAGDYAASHHDTLCYTLALPIVSLPTYYLFSLFGDDFRLAVVMLWSALLLA
                     LLLMIEFWFPQYARWREIPWTWAGIISWGILLVLNAALYRQFWFVRGVQPYDVGVYPE
                     VAAIVFTNSIAFALLAVVAFLIFREVLGSDRWGLFGLAATVCCSSYLFWSGNAKDHAL
                     VALLFAVALYCFTLYLSRENPLYLFASFVAVGWTAFARPELGPALAAGFFLFGVAVAA
                     GKGRRETGKAVLAAAGVPLGALPLLVNNWGLSGHPLVLPWVAGYAAAGEKALPSGSES
                     LWSTVIGHYTPPPGDLLTGLYGAFFDPVRDGSAAFFQVSPLSFFA"
BASE COUNT          720 a         1223 c         1138 g          816 t
ORIGIN      
        1 tgcgtcgccg cacgcctctc gtgcctttgc cgcgaattcg tggatgaccg gtcgcttgac
       61 cgaaaccata tgatggagtt gttgcgaatg cagggccata ataattactg atcaaacctt
      121 cacggccggg acgaccggcc gggctccggc cggaccccta ccggcacgtc cggaaaccgg
      181 ccgggttttt ctaggactcc tatctccgga tccgggtggg ggccggtacc gggtcatcct
      241 cacgatcgaa gacgagcgcc tcctgatccg gcgggacacc ggagcagcac ctcccgacag
      301 ggagacgatc aagaaccatg gccgttcccg gtcttctgcc gcctatggaa cggtgcggta
      361 gggggcgtgc tccaggataa gatttatagg tctggtggcg atgtatttac tactggagat
      421 atgagcccgg gaaccgacat ctataccctt ctcatccaga gacgggacga gatcctcact
      481 ctcgcgcacc ggaggggggc aaagaacgtc cgggttttcg gatccgtggc gcggaaggaa
      541 gcccgggaag acagtgatat cgatatcctg atcgatctgg atcccgaccg gagtcttctc
      601 gacgtcgggg gtcttgcgat ggacctttcc ctcctgctcg accgtccggt cgacgttgtg
      661 accgaagcgg gactccggga gcggatccgc tcaagggtcg tacgggaagc gcgggctcta
      721 tgagagacga tccttgagcg ggttctggat atcctggaag ccatcgagcg aattgaacgg
      781 gtgtcggtgc ggggccgaga gtttttctac gatgacgaaa tggtacagcg tcccggatga
      841 ttcaccacct cctgatcatc ggggaagcag tgcgggggat ctctctggag ttcaggactg
      901 ccaacccgga cattccgtgg tcggatatcg tcgggatgcg gaacgtcctg gttcatcact
      961 attttgggat cgaccggaat gcggtctgga acgtggtcga gcacgatctc ccggcgctga
     1021 aggaacaact cttacccttg atccaggagt agcggcttag gtagggttgc cagtacacag
     1081 ttcacgttac aggagaggat cgaacgctgc cctatcgcat gcttcatatc ctataatgac
     1141 ctatagagat ttacatgtcc gccactaccg tctggatcac accggcgaac aaagaccgcc
     1201 tggagggcct gaaacggcac cccagggagt cctataacga cgttatcggc cgcctgctcg
     1261 acatgaccgt cgacgacgag cccctctcgg aggaggccat ccggggtatc gaggaagccc
     1321 tggaggacat caaggccggc cggctctact ccgaggacga tatccggaag gagtttggtg
     1381 tggaggaatg acctatacgg tcacctacac cgcccgggca cggcgggatc ttcagaatct
     1441 tcctcccgaa gcggcccgcc ggatcatccg ttcggtcgcc gggattcggg agaaccccta
     1501 ccggcacgtc cggaaactgg ccgggttttc caggactccc gtctaccggc tccggataga
     1561 tcggtaccgg gttattctca cgatcgagga tgagcgcctc ctgattcttg tactggaggc
     1621 gggagaccgg agcagcatct accggtagaa tccttcgcgg atccggcgcc acatacacca
     1681 tatcgccgga ctggatctgc acgaaaaaga gccgggctgc ggtgaatgtc gaacgggtcc
     1741 tgctgtaccg gcatatcggc ggccgaatcc atagggatat ccccggggaa cggcgtgctg
     1801 cctacgggag aaagatagtc tcgacagtgt cgcgagaatt ggtagcagtt gtacgggacg
     1861 gtgcaacccg ccggccgggg tgcggcgggg gggggcggtg ctcgtgctcc ggacgtcgtt
     1921 ggcgggcagt cccgggtcgc acctgccggt gctcgtgctc cggtgtcgcg cagcataaga
     1981 aatccgggcc cgctacggcc ggggatgctc tccacctgcc gcaccccggg cggctctgcg
     2041 atggattttg ggccgggtga acccgtatta tccctgaaat tgcgatcccg gcccgtttgt
     2101 tctttgcaac aactccttta tatcatgaaa ggatctttcg cagtatccgg ggacggcatt
     2161 attaaatcgt tcgggaaagg atcactacga taatattata tataatgaga aagtagttag
     2221 tctcgtcgac tgaaagcctc atcaggggcg tgaggtcaaa gaggtgtaaa agtatgatga
     2281 aattcagaga gaatgaagat gcagtatcac cggtgatcgg cgttatcctc atggtggcca
     2341 tcacggtgat cctcgccgcg gttatcgcgg cgtttgtgtt tgggatgacg gggaacgtgc
     2401 agacgacgaa gacggttgcg gttaccgcca atcagaacag caataatgtc gtcctgacct
     2461 accatggcgg tgctgatgca gccacactcc agtacctgac gattactgta acaaaaggta
     2521 gtgacacaac ttctgagacg cagtatcttt ggtacgatac aagcagttct ggggtggtga
     2581 cgaccaaccc taatgagaat aatgttggga aactccccgt aggcaccacc acaaccatcg
     2641 ttgggtttgc ttcatctcaa caggaccacg tcgttgttgc aggtcacttc gctgacggct
     2701 ctgatcatgt cgtgctcgac acttacgtgt aagcacccct catgaacaac catccttttt
     2761 tgaaaaaaat actattcatc acgtttatct gctgccttct ctccctcgcc atcggctacc
     2821 ccctcctcta cctcaccgac gaatggatca ccgccgacca actcaaccac ctcatcaccg
     2881 gcgacgacct cctcttcggc tacgagccct acggagcagg tgactacgcc gcctcccacc
     2941 acgacaccct ctgctacacc ctcgccctcc ccatcgtttc gctccccacc tactacctct
     3001 tctcgctctt tggtgacgac ttccgcctcg ccgtggtcat gctctggtcc gccctcctcc
     3061 tcgccctcct cctcatgatc gagttctggt tcccgcagta tgcccgctgg cgggagattc
     3121 cctggacctg ggccgggatc atctcctggg ggatactcct agtcctcaac gcggcgctct
     3181 accgacagtt ctggttcgtg cggggggtgc agccctacga cgtgggggtc taccccgagg
     3241 tcgccgcgat cgtcttcacg aacagcatcg ccttcgccct cctcgcggtc gtcgccttcc
     3301 tgatcttccg ggaagtcctc ggctccgacc ggtgggggct cttcgggctc gccgcgacgg
     3361 tctgctgctc ctcgtacctc ttctggtccg ggaacgcaaa ggaccacgcc ctcgtcgccc
     3421 tcctcttcgc cgtcgcgctc tactgcttca ccctctacct ctcgcgggag aacccgctct
     3481 acctctttgc gtcgttcgtc gccgtcgggt ggacggcgtt tgcccgtcct gaactcggcc
     3541 cggcgctcgc cgccgggttc ttcctcttcg gggtcgccgt cgcggccggg aagggccgtc
     3601 gggagaccgg gaaggccgtc ctcgccgccg ccggggtccc gctcggcgcc ctcccgctcc
     3661 tcgtgaacaa ctgggggctc tccggccacc cgctcgtcct cccctgggtt gcgggctacg
     3721 ccgcagccgg ggagaaagcg ctcccctccg ggtcggagag cctctggagc accgtgatcg
     3781 ggcactacac cccgccgccc ggcgacctcc tcaccggcct ctacggcgcc ttcttcgacc
     3841 cggtccgcga cggctccgcc gcgttcttcc aggtctcgcc gctctcgttc ttcgccc
//
LOCUS       DAOSEE010000178         3852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_8329_length_3852_cov_247.161180, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000178 DAOSEE010000000
VERSION     DAOSEE010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8329_length_3852_cov_247.161180"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..507
                     /locus_tag="PLI76_10310"
     CDS             175..507
                     /locus_tag="PLI76_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62468.1"
                     /translation="MRKCVRSEKGEIFLDTDRDTWLYLAPRGGDAGGAFARGKDLYLH
                     EQEGLPDTYYIYSWSMDPGEEEQIQTVSLISAEEFLELRGLVLNAYPEQRGSQILRSY
                     GYGIAEEF"
     gene            612..2654
                     /locus_tag="PLI76_10315"
     CDS             612..2654
                     /locus_tag="PLI76_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase subunit D family protein"
                     /protein_id="HOI62469.1"
                     /translation="MSYQVRRNVLPFTAIVGQETMKRALILNAVNPGIGGVLVRGEKG
                     TAKSTAVRALADVLPGIDVVRGCPYNCDPDGCLCTACAEKKASGEDLEAEHRRVRVVD
                     LPLGATEDRVVGTVDVERAIREGKVTIDPGLLAAVNRGVLYIDEVNLLDDHVADVLLD
                     VAAMGVNVVEREGISFSHPARFILIGTMNPEEGELRPQLLDRFGLQVTVEGIEDPAQR
                     VAIVRAAEVFERDPGGCRQTYGAAQEKLRREIVAARMLLPSVSVDDTLLDAVAKACIR
                     LGVRTHRADIAVVRTAKTIAALDGRTVVTRDDVREAMELALPHRMRRKPFEEPRIDPE
                     ELERALENAGEGHDRAPGDNGDGAPGNGPSGGHDGDPPPSGGNAAHETVLPVGDPVDP
                     NKVSMPQRRDARERQRASGRRFETFSVKNTGKYVSARYPAGTGDIALDATLRAAAPRQ
                     AVRDREGVAIAVRREDIRERVRVGKVSVACVFVVDASGSMGAARRMESAKGAVLSMLL
                     SSYRHRDRIGLVAFRGSSAETLLPLSTSIDLAQKQLKELPTGGKTPLAAGLMKGMEVL
                     LQERQKNGEVIPMMVVVSDGRANVSLSGDVCQEVLDIAEEIRLQGIRTVVIDTEETGN
                     SFLEFRLGYCRKIAEHAGGGYYPIADLSAGLLHDVAAAERDAMVHPARKPLTGRAA"
     gene            complement(2635..>3852)
                     /gene="purB"
                     /locus_tag="PLI76_10320"
     CDS             complement(2635..>3852)
                     /gene="purB"
                     /locus_tag="PLI76_10320"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOI62470.1"
                     /translation="HGMIPREDAETIAACALEARLERAKEIEAEINHDMMAVVKAVAE
                     VCGDAGRWIHYGATSNDILDTATALQLRDSLALIEEKLGKLLCVLLARSAETKTLVCA
                     GRTHGQIGVPTTYGLRFAIWASEVARHIERLRQMRPRVVVGHLTGAVGTQAALGDAGI
                     DIQATMMEFLGIRSVDVSNQIIARDRYAEYFMFLANVATTLDKIGLEIRLMQRSEIGE
                     LAEAFGKKQVGSSTMPHKRNPIKSEQVCGLARIVRSAVEPALQNNVLWDERDLTNSSP
                     ERVLFPEASVLADHILNVMIGVMDGLEFNKKNIRKNLMLLRGVNLAESVMIELTKRGM
                     SRQEAHEAMRTASMQALAEDRDLSEVLGERPEITTFVTREELDRLLDPDTYIGTAVRQ
                     VERVIEKLTPPCR"
BASE COUNT          669 a         1223 c         1272 g          688 t
ORIGIN      
        1 attgtagtac ttaatcttaa aaatagtaag ttatataaca ctcgccgttc tacccctgcc
       61 tggcgggcgg tggctggatc atcgataagc cagggccacc tcaccgggct tccggggttc
      121 attgccgctg tctgccgaat cgccctgcct gtggcggggc ggcggtgatt gatcatgaga
      181 aaatgcgttc gatccgaaaa aggcgagatc ttccttgaca ccgaccgtga cacctggctc
      241 taccttgcgc cacggggcgg tgacgcaggc ggtgcatttg cgcgcggaaa agacctttac
      301 ctgcacgagc aggaggggtt gcccgatacc tactacatct actcctggtc gatggacccc
      361 ggcgaggaag aacagatcca gaccgtctcc ctcatctccg ccgaggagtt cctggaactg
      421 cgggggctcg tgctcaacgc atacccggaa cagaggggga gccagatact gcggtcatac
      481 ggctacggca tcgcggaaga gttctgagac ctcgcggtcc cgggtggggg cagggctacc
      541 ctctcccgcc atacccatta catgttacga aatcgaaatt ctttaacatc tattgcatcg
      601 tattctcatg tatgtcttac caggtccgga ggaacgtcct tccgttcacc gccatcgtcg
      661 gccaggagac gatgaaacgc gcgctcatcc tcaacgccgt caacccgggg atcggcggcg
      721 tcctcgtccg cggcgagaaa gggaccgcaa agtccactgc agtcagggcg cttgccgacg
      781 tgctccccgg gatcgacgtg gtccggggct gcccgtataa ttgtgatccc gacggctgcc
      841 tgtgtactgc ctgcgccgaa aaaaaggcct ccggtgagga ccttgaagcg gagcatcgcc
      901 gggtccgcgt cgtcgacctc ccgctcgggg caaccgagga ccgggtggtg ggcaccgtcg
      961 atgtggagcg ggcgatccgc gaggggaagg tgacgatcga tccgggcctc ctcgcggcgg
     1021 tcaaccgcgg ggttctctat atcgacgagg tcaacctgct cgacgaccac gtggccgacg
     1081 tcctcctcga tgtcgccgcc atgggcgtca acgtcgtgga gcgggagggg atatcgttct
     1141 cccaccctgc ccggttcatc ctgatcggca ccatgaaccc ggaggagggg gaactccggc
     1201 cgcagttgct cgaccggttc gggttgcagg tgaccgtgga ggggatcgaa gatcccgcgc
     1261 agagagtggc gatcgtcagg gccgccgagg tattcgagcg cgatcccgga ggatgccggc
     1321 agacgtacgg cgcggcgcag gagaagttgc gcagagagat cgttgccgca cggatgctgc
     1381 tcccctccgt ctcggtcgac gacaccctcc tggatgcggt ggcgaaggcg tgcatccggc
     1441 tcggagtccg gactcaccgc gccgatatcg ccgtcgtccg cacggcaaag acgatcgccg
     1501 cccttgatgg gcggacggtc gtcacccgcg acgacgtccg ggaagccatg gaactcgccc
     1561 tcccgcaccg gatgcgccgt aaaccgttcg aagagccgag gatagacccg gaggagcttg
     1621 agagagccct ggagaacgcc ggggaggggc acgatcgcgc tcccggggac aatggagatg
     1681 gcgcgcccgg caacgggccc tccggcgggc atgacgggga tccgccccct tccggcggga
     1741 acgccgcgca cgagacagtt ctcccggtcg gggaccctgt cgatcccaat aaggtgagca
     1801 tgccgcagag gcgggatgcc cgggagcggc aacgggcctc cggcaggcga tttgagacgt
     1861 tctcggtgaa aaatacgggg aagtacgtct ccgcacgtta ccccgccggc accggcgata
     1921 tcgcgctcga cgccacgctt cgggccgccg ccccgcgcca ggcggtgcgg gaccgggaag
     1981 gggtggcgat cgccgtccgg agagaagata tccgcgagcg ggtccgtgtg gggaaggtct
     2041 ccgtcgcgtg cgtcttcgtg gtcgacgcca gcggctcgat gggagccgca aggcgaatgg
     2101 agtcggccaa gggtgcggtc ctctcgatgc tgctctcctc ctaccggcac cgcgaccgca
     2161 tcggtctcgt ggcgttccgg gggagcagcg ccgagaccct cctcccgctc tcgacaagca
     2221 tcgatctcgc gcagaaacag ttgaaggagc ttcccaccgg gggaaagacg ccgcttgccg
     2281 ccggcctgat gaagggcatg gaggttctcc tgcaggagcg gcaaaagaac ggcgaggtga
     2341 tcccgatgat ggtcgtcgtc tccgacggcc gggcgaacgt ctcgctctcg ggcgacgtgt
     2401 gccaggaggt tctcgatatt gcagaggaga tccggttgca gggcatccgc acggtggtca
     2461 tcgataccga ggagaccgga aactccttcc tggagttccg gctcggttat tgccggaaga
     2521 tcgcggagca cgccggtggc ggctactacc caatcgccga cctctctgcc gggctcctgc
     2581 acgatgttgc tgcggccgag cgggatgcga tggtgcaccc cgcccgaaaa ccgctcaccg
     2641 gcagggcggc gtaagtttct cgatcacccg ctccacctgc cggacggccg tcccgatgta
     2701 agtgtccggg tcgaggagcc ggtcgagttc ctcgcgggtg acgaacgtcg tgatctccgg
     2761 ccgctcgccg agcacctccg agaggtcgcg gtcttcggcg agtgcctgca tgcttgccgt
     2821 ccgcatcgcc tcgtgggcct cctgccggct catgccccgc ttcgtcagtt cgatcatcac
     2881 cgactcggcg aggttcaccc cgcgaaggag catcaggttc tttcggatgt tcttcttgtt
     2941 gaactcaagg ccgtccatca cgccgatcat cacgttgagt atgtgatcgg cgagcaccga
     3001 ggcttcgggg aagagcaccc gctcggggga tgagttcgtg aggtcgcgct cgtcccagag
     3061 gacgttgttc tgcagggccg gctcgaccgc agaccggacg atccgtgcaa gcccgcagac
     3121 ctgctcgctc ttgatcgggt ttcgcttgtg gggcatcgtg ctcgagccca cctgcttctt
     3181 cccgaacgcc tccgcaagtt ccccgatctc tgaacgctgc atcagccgga tctcaagacc
     3241 gatcttgtcg agcgtcgtcg cgacgtttgc aaggaacatg aagtactccg cgtagcggtc
     3301 ccgcgcgatg atctggttag agacatccac ggaccggata ccgaggaact ccatcatcgt
     3361 cgcctggata tcgatgccgg catccccgag ggccgcctgg gttcccacgg cgccggtgag
     3421 gtgcccgacg acgacgcggg ggcgcatctg gcggagccgc tcgatgtggc gggcgacctc
     3481 gcttgcccag atggcaaacc ggaggccgta ggtggtggga acaccgatct gcccgtgggt
     3541 gcggccggcg cagacgaggg tctttgtctc ggcgctccgg gcaagaagca cgcagaggag
     3601 tttacccagc ttctcctcga tgagcgcaag gctatcgcgg agctgcaggg cggtcgccgt
     3661 atccagtatg tcgtttgatg tcgccccgta gtggatccat ctgcccgcgt cgccgcagac
     3721 ctcggcgacg gccttgacga ccgccatcat gtcgtggttg atctcggcct cgatctcttt
     3781 tgcccgctca agacgagcct cgagcgcaca tgccgcgatc gtctccgcat cctcgcgggg
     3841 gatcatcccg tg
//
LOCUS       DAOSEE010000179         3846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_8352_length_3846_cov_255.387233, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000179 DAOSEE010000000
VERSION     DAOSEE010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8352_length_3846_cov_255.387233"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..820
                     /locus_tag="PLI76_10325"
     CDS             92..820
                     /locus_tag="PLI76_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4e"
                     /protein_id="HOI62471.1"
                     /translation="MSNHLKRLVAPGSWHIPKKVQKFVMKTAPGPHNAGALPVGVWLR
                     EHIGLAGNASEVRKILHQRDVIVNGRACRNPQMGLGVFDIVSIPKLAKHYRIQLDKRG
                     NLIAVDISAASAKTRLCKIRNKTTVRGGRVQLNLAFGANVLADNTYRAKDSIVVTLGT
                     DGEERFRIVDHFPFEEGNVAMVVGGKHSGKVARIVEIARTASSVPNRVILVDDSTDER
                     FETIEEYIFMVGRTAIAPELEASA"
     gene            817..1320
                     /locus_tag="PLI76_10330"
     CDS             817..1320
                     /locus_tag="PLI76_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOI62472.1"
                     /translation="MSAMKDIYIDKVVVHMGVGESGERLVKAEDLVKKITGQKPVRTI
                     AKRTQPAFGIRKGAPIGCKVTLRRANAEKFVETALAIVERHLAASQFDRTGNVSFGIE
                     EHTDFPGMAYDPAIGIFGMDVNVVLERKGVRIARRSVERRKLPAKQRVNQEEAIAFMR
                     ERYQVEV"
     gene            1324..1503
                     /locus_tag="PLI76_10335"
     CDS             1324..1503
                     /locus_tag="PLI76_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HOI62473.1"
                     /translation="MAEKSGASAPGAGVKRFGRGANECRICGRKQGLVRKYGIYFCRQ
                     CFREWAGRMGFKKMS"
     gene            1517..1909
                     /locus_tag="PLI76_10340"
     CDS             1517..1909
                     /locus_tag="PLI76_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOI62474.1"
                     /translation="MARLNPIADAMSTIKNAGDAGKSEVIVEPAGKLLGAMLRVMQEN
                     GFIGGFEFIDDGRGGQFRVQLPGTINKCGAITPRFSVGMADMEYWESQYLPAKNFGIL
                     IVSTSKGVISHEQARGEGIGGQLLGYVY"
     gene            1924..2448
                     /locus_tag="PLI76_10345"
     CDS             1924..2448
                     /locus_tag="PLI76_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOI62475.1"
                     /translation="MGILRQIEIPPGVNARLEGSVLTVSGPKGTLVRDMRFPQIDVRI
                     ADGEVIVSTASDKRRFLAMSGTLEAHVKGMFRGVVEGYEYRMKVVYSHFPIQLKLQGD
                     RLEIGNFLGEKQPRAAKIIEGVTVRIGNDEVTLTGIDREKVGNTAANIEHATKITKRD
                     PRVFQDGIYITERA"
     gene            2454..2879
                     /locus_tag="PLI76_10350"
     CDS             2454..2879
                     /locus_tag="PLI76_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32e"
                     /protein_id="HOI62476.1"
                     /translation="MDETRRLIRARARHNKPAFKRRGLHRKAKLEDVWRRPRGLHSKQ
                     RRQFKAKGALPRPGYGSPAAVRGMHPSGYEEVRIFTPAELAGLNPESQAVRIAGAVGN
                     RKRAEIQTRALELGLKVLNAKDLVRAVEAPVEEEADEDE"
     gene            2872..3324
                     /locus_tag="PLI76_10355"
     CDS             2872..3324
                     /locus_tag="PLI76_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19e"
                     /protein_id="HOI62477.1"
                     /translation="MSDLANQKRMAAAILKCGVNRVWFDPERQTDIEDAISRSDLRTL
                     IEEGAIRAHAVKGNSRGRARARMAQRSYGHRKGPGRRKGAAGARASSKRTWIGKIRAQ
                     RRALRDMRGEGRIERSLYRVMYRRASGGQFRSVAHLEAQVEIAAGRMR"
     gene            3324..>3846
                     /locus_tag="PLI76_10360"
     CDS             3324..>3846
                     /locus_tag="PLI76_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOI62478.1"
                     /translation="MATGPRYFVPFRRRHEGKTDYYRRMSLLSSGSPRMVVRKTNRQI
                     IVQLVVPGSEGDRTLVAAYSAELAGYGYEGSTASTPAAYLTGMLFAVKALNAGYEEGI
                     LDIGLARAKSGARVFAALKGAVDGGLDIPYGESILPDEERLKGAHIAEYAPEQAGNLV
                     ANVEAVALAIKKEL"
BASE COUNT          851 a         1106 c         1275 g          614 t
ORIGIN      
        1 ttccccggcc ggtcgatccc tcgaacgtgc agatcacgaa gctcaacctg aaagataaac
       61 tccgcgagga gagactcgga ggcggagcat aatgtccaat catctcaagc ggctggtagc
      121 gccgggatcc tggcatatcc cgaagaaagt acaaaaattc gtcatgaaga ccgctccggg
      181 gccccacaac gccggcgccc tgccggtcgg tgtctggctc cgcgagcata tcggccttgc
      241 agggaacgca agcgaggttc gaaagatcct ccaccagcgc gacgtcatcg tcaacgggcg
      301 ggcctgcagg aacccccaga tgggccttgg tgtctttgac atcgtctcga tcccgaagct
      361 tgcaaagcac taccgcatcc agctcgacaa gcggggcaac ctgattgccg tcgatatatc
      421 tgcggcgtcc gcaaagaccc ggctctgcaa gatccggaat aagaccaccg tcaggggcgg
      481 cagggtacag ctgaaccttg cgttcggcgc gaacgtactt gccgacaaca cctacagggc
      541 aaaagactcc atcgtcgtga cccttggaac ggacggcgaa gaacggttcc ggatcgtcga
      601 ccactttccc tttgaggaag gcaacgtggc catggtcgtc ggcggaaagc actccgggaa
      661 ggtcgcgagg atcgtcgaga tcgcgaggac cgcaagctcc gtcccgaacc gggtgatcct
      721 cgtggacgac tcgaccgacg agcggttcga gaccatcgag gagtacatat tcatggtcgg
      781 ccgcaccgcg atcgcacccg aactggaggc ctcggcatga gcgcgatgaa ggatatctac
      841 atcgacaagg tcgtcgtgca tatgggggtc ggagagagcg gcgagcgtct ggtcaaggcc
      901 gaggatctcg tgaagaagat caccggccag aaacccgtcc gcaccatcgc aaagcggacc
      961 cagccggcgt tcggcatccg gaagggcgca cccatcgggt gcaaggtcac cctgcgccgg
     1021 gcaaacgccg agaagttcgt cgagaccgcg cttgccatcg tcgagcggca ccttgccgcc
     1081 tcgcagttcg accggacggg taacgtctcc ttcggcatcg aggagcacac ggacttcccc
     1141 ggcatggcct acgatccggc aatcggcatc ttcggcatgg acgtcaacgt ggtgctcgag
     1201 cgcaaaggcg tgcggattgc acggagaagc gtcgagcgca ggaaattgcc ggcaaagcag
     1261 agagtgaacc aggaagaagc catcgcgttc atgcgcgagc gctaccaggt ggaggtgtaa
     1321 gggatggcag aaaagtcagg tgcatctgct ccgggcgcgg gtgtaaagag gtttggccgt
     1381 ggcgcgaacg agtgccgcat ctgcggccgg aagcagggcc tggtgcgtaa atacggcatc
     1441 tacttctgtc gccagtgctt ccgcgagtgg gccggaagga tgggctttaa gaagatgagc
     1501 taaggggtga gagaatatgg cacgactgaa tccaatcgct gacgcgatga gcacgatcaa
     1561 gaatgccggc gacgctggaa agagcgaggt tattgttgaa cctgccggca agctcctggg
     1621 cgcaatgctc cgcgttatgc aggaaaacgg ctttataggc ggcttcgagt tcatcgacga
     1681 cggccgcggc ggccagttcc gggtccagct ccccggaacg atcaataagt gtggcgccat
     1741 caccccccgg ttctcggtgg gtatggccga tatggaatac tgggagtcgc agtacctccc
     1801 cgcgaagaac ttcggtatcc tgatcgtctc gacctcgaag ggagtcatct cccacgagca
     1861 ggcccggggc gaaggtatcg gcgggcagct gctgggatac gtctactgac ggaggagaga
     1921 ctcatgggaa tactacgaca gatcgagatc cctcccggcg tgaacgcgag actcgaaggc
     1981 agcgtgctca cggtatcggg gccgaaaggc acgctggtcc gtgatatgcg tttcccgcag
     2041 atcgacgtca ggatcgccga cggcgaagtc atcgtctcca cggcatccga caagaggcgg
     2101 ttccttgcca tgagcggcac gcttgaggcg cacgtgaaag gcatgttccg gggcgtcgtc
     2161 gaaggttacg agtaccgcat gaaagtggtc tacagccact tcccgatcca gctcaaactg
     2221 cagggcgacc gtcttgagat cggtaacttc ctcggcgaga aacaaccgcg ggcggcaaag
     2281 atcatcgagg gcgtcaccgt ccggattgga aacgacgagg tgacgctcac cggtatcgac
     2341 agagagaagg tgggcaacac cgccgcaaac atcgagcacg cgacgaagat cacgaagcgt
     2401 gatccccggg tgttccagga cggcatctac atcaccgaga gggcgtgaag agaatggatg
     2461 aaacacgaag attgatccgc gcccgtgccc ggcacaacaa acctgccttc aagaggcgag
     2521 gactccaccg caaagcgaag ctcgaagatg tctggcggcg gccgcgcggt ctgcacagca
     2581 agcagagacg acagttcaag gcaaagggag cgcttccccg gccggggtac gggagccctg
     2641 ccgcagttcg cggcatgcac ccgagcggct acgaggaagt ccggatcttt acgccggcag
     2701 aacttgccgg gttgaacccg gagagccagg ccgtccggat cgccggagcg gtaggcaaca
     2761 gaaagcgcgc agagatccag acccgcgccc tggaactggg gctcaaggta ctgaacgcaa
     2821 aagatctcgt ccgtgcggtc gaagcgccgg ttgaagagga ggcggatgag gatgagtgat
     2881 cttgccaacc agaagcggat ggcggccgcc atcctcaagt gcggggtcaa ccgtgtctgg
     2941 ttcgatcccg agcgccagac cgatatcgag gatgccatct cccgaagcga cctgcggacc
     3001 ctcatcgagg agggtgcgat cagggctcac gccgtgaagg ggaacagccg cggcagggcg
     3061 cgtgcacgaa tggcacagcg ttcctacggc caccggaagg gcccgggtcg gaggaagggt
     3121 gccgccggtg cccgtgcatc gagcaagcgc acgtggatcg gtaagatccg ggcacagcgc
     3181 cgggctctgc gcgatatgcg cggcgaaggc aggatcgagc ggagtctcta ccgcgtgatg
     3241 taccggcggg cttccggagg ccagttccgg agcgtggcgc atcttgaggc tcaggttgag
     3301 atagcggcag ggaggatgag ataatggcaa caggcccacg atacttcgtg cccttccgaa
     3361 ggagacacga aggtaagacc gattactaca ggcggatgtc gctcctctcg tcaggcagcc
     3421 cgaggatggt cgtccggaag acgaaccggc agatcatcgt gcagctggtc gtccccggga
     3481 gtgagggaga tcgcaccctg gtggctgcgt actcggccga gcttgccggt tacggctacg
     3541 agggatcgac cgcgagcact ccggccgcct acctgaccgg gatgctgttt gcggtcaagg
     3601 cgttgaatgc agggtatgag gagggtatcc tcgatatcgg gcttgcccgg gcaaaatccg
     3661 gggcgcgtgt ctttgccgcc ctcaaaggag cggtggacgg cggcctcgat atcccctacg
     3721 gcgaatcgat cctccccgat gaagaacggc tcaaaggtgc ccacatcgcg gagtatgctc
     3781 ccgaacaggc gggcaacctg gtagcgaatg tagaggctgt ggctctggcc atcaagaagg
     3841 agctgg
//
LOCUS       DAOSEE010000180         3801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_8491_length_3801_cov_208.821676, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000180 DAOSEE010000000
VERSION     DAOSEE010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8491_length_3801_cov_208.821676"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="PLI76_10365"
     CDS             complement(<1..917)
                     /locus_tag="PLI76_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5591 domain-containing protein"
                     /protein_id="HOI62479.1"
                     /translation="MSRYEAQKRDGLARIGIYEEGGTTVSLPAALDTGALFPSLRERA
                     FSNVPLSADPAFVRDYFSPGEGQPVAVHPIAPPAVKSGDCVLVANWHTALKNPRNYAT
                     WLAALKESVPPDTAWYAPGAALPSTACLLAYSGFDLFDYRAVDLASAQRIFCLPEGGF
                     PASVMATGVCGCEGCRDGDLIRHNRLALDRELALIRHYIEAGRLRELMESRCRADAAQ
                     VGILRFLDRNYALMERSLPVVRAVPMLAATAESQNRAEIRRFADRVIERFVPTRTDVA
                     VLLPCSARKPYSFSRSHRLFMNTVDRRAH"
     gene            complement(904..2355)
                     /gene="tgtA"
                     /locus_tag="PLI76_10370"
     CDS             complement(904..2355)
                     /gene="tgtA"
                     /locus_tag="PLI76_10370"
                     /EC_number="2.4.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(15) transglycosylase TgtA"
                     /protein_id="HOI62480.1"
                     /translation="MTITFEVIHKDIAGRVGKLRVNDKAVRTPALLPVVNPHLPLVTP
                     REMQEMGVEALITNAYIFRRSTEFCDRALAKGLHAVLDFDGAVMTDSGSFQLSVYGEV
                     EVSNRETLEFQQAIGSDIIVPLDIPTPPDASRERAEQELAVTMDRIREAQALFPDANL
                     AGPVQGGIFTDLREEAGRAVRDLGVAFAPIGAVVPLMENYRYQDLVRVVLAAKRGLSP
                     ATAIHLFGAGHPSMFALAVAMGCDLFDSAAYALFAREGRYITPHGSLKIDELAELPCP
                     CRVCRSMTADELRQSEDRERLLALHNLHVTLAEIARIRQAIQDGTLWELVDERCRSHP
                     RLLAGYRELLAHAAELERDDPVSKRRFFYRGSETCRRTEVLRFHEVVPRIPLGERVLV
                     TFDGREVPGFDTVLNFKPPFGPYPVELAETFPIGQSEVPDWDDDMVRSGCAGIRALVE
                     AHPEKRFAIRCSDAWVRIVLEEVPGAEVYREQV"
     gene            complement(2361..2927)
                     /locus_tag="PLI76_10375"
     CDS             complement(2361..2927)
                     /locus_tag="PLI76_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00296 family protein"
                     /protein_id="HOI62481.1"
                     /translation="MEMLTPEEGRTVVRLARSAIEKTLSGERAVLPDLPAVFREKRGV
                     FVTIKRQGHLRGCIGLPYPIKPLGEAVVEAAISAALEDPRFPPVSRSEVAGIDLEVTV
                     LTEPRPLDCPPDERPGCVEVGRHGLIVSGHGTGGLLLPQVPAEYGWTSREFLDQTCIK
                     AGLPPGCWKRGSVAVLTFEGQIFDEKAV"
     gene            complement(2957..3508)
                     /locus_tag="PLI76_10380"
     CDS             complement(2957..3508)
                     /locus_tag="PLI76_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62482.1"
                     /translation="MGGSEASTQLELAAIFAAFVAVTTLFSFVSIGANALGGKIPEGA
                     GVYPVLAAGEPRLAPVGDIIGYPSVPGPSGTRIDALTFVIANTGGEGAVDLSRATVTV
                     MAGNYLEVLTRSEDLHPEPGAWTTSLPRDSDGNVLLRAGEECAIRISLGHLIPAGETV
                     TVRVRLPGDRPCLIAGQAGHPED"
BASE COUNT          661 a         1221 c         1299 g          620 t
ORIGIN      
        1 tcgtgcgccc gccggtccac cgtgttcatg aagagcctgt ggctccgtga gaacgagtag
       61 ggtttccgcg cagaacaggg gaggaggacc gcgacgtcgg tccgggtcgg gacgaaccgc
      121 tcgatcaccc ggtcggcgaa ccgccggatc tccgccctgt tctgcgactc ggcggtggcg
      181 gcaagcatcg gcaccgcccg caccaccggg agggagcgct ccatgagggc gtagttccgg
      241 tcgaggaacc ggaggatgcc cacctgtgcg gcgtccgcgc ggcaccgcga ctccataagt
      301 tcccggagcc gcccggcctc gatgtagtgc cggatgaggg cgagctcgcg gtcgagcgca
      361 agccggttgt gccggataag gtcgccatcc cggcagccct cgcacccgca gaccccggtt
      421 gccatgaccg aggccgggaa cccgccctcg gggaggcaga agatcctctg cgcagatgcg
      481 aggtcgacgg cccggtagtc gaagaggtcg aaacccgagt aggcgaggag gcaggcggtc
      541 gaggggagcg ctgctccagg ggcgtaccac gcggtgtccg gggggacgct ctcttttaag
      601 gccgcaagcc atgtcgcgta gttccgcggg ttcttgagtg ccgtgtgcca gttcgcgaca
      661 aggacgcagt cgccggactt taccgccggc ggggcgatcg ggtggacggc gaccggctgc
      721 ccctcgccgg ggctgaagta gtcgcggacg aacgccgggt cggcggagag cgggacgttt
      781 gagaaggcac gttcgcggag cgacgggaag agggcacccg tatcgagggc ggcggggagg
      841 gagacggtcg tccctccctc ttcatagatc ccgatccgtg ccaggccgtc gcgcttctgc
      901 gcctcatacc tgctcacggt acacctccgc accggggacc tcttcgagga cgatccttac
      961 ccacgcatcg ctgcaccgga tggcaaacct cttctccggg tgcgcctcca ccagggcccg
     1021 gataccggcg catcccgacc ggaccatatc gtcgtcccaa tccgggacct cgctctggcc
     1081 tatcgggaac gtctccgcga gttccacggg atacggcccg aaagggggct tgaagttcag
     1141 caccgtatcg aatccgggga cctcgcgccc gtcaaacgtc accagcaccc gctcgccgag
     1201 gggaatgcgg gggacgacct cgtggaaccg gagcacctcc gtccgccggc aggtctccga
     1261 gccccggtag aagaaccggc gcttggagac ggggtcgtcg cgttcgagtt cggccgcgtg
     1321 ggcgaggagt tcccggtagc cggcaagcag gcgcgggtgg ctccggcacc gctcgtcgac
     1381 gagctcccag agggtcccgt cctggatcgc ctgccggatg cgggcgatct ccgcgagggt
     1441 gacgtggagg ttgtggaggg caagcagccg ctcccggtct tccgactgcc ggagttcgtc
     1501 ggcggtcatc gagcggcaga cccggcaggg gcaggggagc tccgcaagtt cgtcgatctt
     1561 gaggctcccg tgcggtgtga tgtagcgccc ttcccgggca aagagagcgt atgctgccga
     1621 atcgaagagg tcgcagccca tcgcgacggc aagggcgaac atcgacgggt ggcctgcacc
     1681 gaagaggtgg atcgcggtcg ccggggagag gccgcgtttg gccgcgagga cgaccctcac
     1741 caggtcctgg tagcggtagt tctccatcag cggcaccacg gccccgatcg gagcgaaagc
     1801 cacgccgagg tcccggacgg cacgcccggc ctcctcgcgg agatcggtga agatcccgcc
     1861 ctgcaccggc cctgcgaggt ttgcgtcggg gaagagggcc tgtgcctccc ggatccggtc
     1921 catcgtgacc gcgagttcct gttcggccct ctcccggctt gcatccggcg gggtggggat
     1981 atccaggggg acgatgatat cgcttcctat cgcctgctgg aactcaagcg tctcccggtt
     2041 gctcacctcg acctcgccgt agaccgagag ctgaaacgag cctgagtcgg tcatgacggc
     2101 gccgtcgaag tcgaggaccg cgtgaagccc tttcgcaagc gcccgatcgc aaaactccgt
     2161 gctccgccgg aagatgtagg cgttcgtgat cagggcctcg acgcccatct cctgcatctc
     2221 gcggggggtc accaggggga ggtgcgggtt gacgaccggg aggagcgccg gcgtccggac
     2281 cgccttatcg tttaccctga gtttcccgac ccttcccgct atatccttgt ggatgacctc
     2341 aaacgtgatt gtcattcaga ttaaaccgcc ttttcatcga atatctgccc ctcgaatgtc
     2401 agcacggcga cgctgccgcg cttccagcat ccgggcggaa ggccggcctt gatgcaggtc
     2461 tggtcgagga actcccggct ggtccagccg tactcggccg gaacctgggg gaggaggagg
     2521 cctcctgtcc cgtgaccgct gacgatcagg ccgtgtctcc cgacctcgac gcaaccggga
     2581 cgttcatccg gggggcagtc gagcggccgg ggttctgtga gcaccgtcac ctcgagatcg
     2641 ataccggcaa cctccgaccg cgataccggc gggaaccggg ggtcctcgag agcagccgat
     2701 atggccgcct cgacgacagc ctcgccgagg ggtttgatcg ggtacgggag gccgatgcac
     2761 ccgcggagat gtccctgccg cttgatcgtg acgaagacgc cgcgcttctc gcgaaagacc
     2821 gccgggagat cgggcagtac tgcccgctcg ccgctgaggg tcttttcgat ggcgctccgt
     2881 gccagacgaa ctaccgttct gccttcttcg ggggtcagca tttccatcag aatggggcgt
     2941 atgatgttga atacttttag tcttccgggt ggccggcctg cccggcgatc aggcagggcc
     3001 ggtcaccggg aaggcgcacc ctgacggtca cggtctcccc ggcggggatg aggtggccaa
     3061 gggagatacg gatcgcacac tcctcgcctg cccggaggag cacgttgccg tcgctgtccc
     3121 ggggcagcga cgtcgtccac gcgcccggtt ccgggtggag atcctccgat cgcgtgagaa
     3181 cctcaaggta attccccgcc atgaccgtca cggtggcccg cgagaggtcg accgccccct
     3241 ctccgccggt attggcgatg acgaaggtca gggcgtcgat ccgggtgccg gatggccccg
     3301 ggaccgatgg ataaccgatg atatccccga ccggcgcaag acgcggttcg ccggcggcca
     3361 ggaccgggta tacacccgca ccctccggga ttttaccccc gagagcattc gccccgatag
     3421 agacgaacga gaagagcgtc gtaacggcaa cgaacgccgc gaaaattgcc gcaagctcca
     3481 gctgtgtaga tgcctcactt ccccccatgg tgataacgcc ggtggtaaga acagaggttg
     3541 attatataaa cctatcccat gcccgggggt atgcgcggcc gatccggtga cttttgaagg
     3601 gatccgcagt gcaagacgat ggaggcggat caccgtactg atatatattt gctccgcacc
     3661 atagtataca ggagagggag agtgcggccg acggtggcac atgctctgtg ctgctgagga
     3721 aagtcctccc accggccaga cacgcagccg catgcaagtg cgggtggcga gagtcacggc
     3781 aatgacacag aaacgacacg g
//
LOCUS       DAOSEE010000181         3791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_8521_length_3791_cov_289.154443, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000181 DAOSEE010000000
VERSION     DAOSEE010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8521_length_3791_cov_289.154443"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..878
                     /locus_tag="PLI76_10385"
     CDS             48..878
                     /locus_tag="PLI76_10385"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit D"
                     /protein_id="HOI62483.1"
                     /translation="MEIAFSRLDERVARFTISGVSTSFANVLRRAMVSEVPTLAIEDV
                     RIYDNTSVIFDEMLAHRLGLVPLRTDLARYAVRSECSCGGAGCPVCTATYTLSVEGPK
                     TVYSSDLISQDPDAAPAEGGIPIIDLGPEQKIVLEAHAIVGTGKEHAKWQATTACGYK
                     NYPVIAVDERCDGCGMCIDECPRDVLEAGQGKVRVVSGRQELCSLCRLCERACLAGGI
                     GTEPAIRIGTEPERFIFVVESDGSMPAREIVERALQYIQRSSDDLIDVINDMTGEGTE
                     "
     gene            875..1240
                     /locus_tag="PLI76_10390"
     CDS             875..1240
                     /locus_tag="PLI76_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18e"
                     /protein_id="HOI62484.1"
                     /translation="MKTTQNKSNPRLAALIVTLKDASRVHEAKIWREIARRLDAPRKN
                     YAEVNLSKIDRYANEGETILVPGKVLGSGALNLPVKVAALDFSEAAVSKITGANGTCM
                     TIEDLLRDNPKGSRVRILR"
     gene            1246..1668
                     /locus_tag="PLI76_10395"
     CDS             1246..1668
                     /locus_tag="PLI76_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOI62485.1"
                     /translation="MVTVIDADGLLLGRMASIVAQRALAGEEIALVNVEKAIVSGNRA
                     HVLAAYYTKRDRGSREGGPFFPRRPDHIVKRTIRGMLPYKRERGIAAFKRVKTYVGVP
                     MQFAGMETESLEAAHIDRLSSPRYITVGAISKNLGAKY"
     gene            1681..2082
                     /locus_tag="PLI76_10400"
     CDS             1681..2082
                     /locus_tag="PLI76_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOI62486.1"
                     /translation="MAKIINTSGKRKTAIARATLKPGNGRVRINSVPLEVYGTELTRM
                     KIAEPLLLIPNALDGVDAAIDVSGGGVVGQAEAVRTALARGIVEWHNDPAIKDAFLAY
                     DRTLLVNDSRQKETKKPHGPGARARFQKSYR"
     gene            2117..2305
                     /locus_tag="PLI76_10405"
     CDS             2117..2305
                     /locus_tag="PLI76_10405"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit N"
                     /protein_id="HOI62487.1"
                     /translation="MIPVRCFTCGKVISTAWKEFERRRDAGEDPKRILDALGLERYCC
                     RRMLLTHKEVVEDLNPYQ"
     gene            2306..2380
                     /locus_tag="PLI76_10410"
     tRNA            2306..2380
                     /locus_tag="PLI76_10410"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2342..2344,aa:Pro,seq:cgg)
     gene            2411..2578
                     /locus_tag="PLI76_10415"
     CDS             2411..2578
                     /locus_tag="PLI76_10415"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit K"
                     /protein_id="HOI62488.1"
                     /translation="MESYTRYERARIIGARALQISMGAPVLVKTPNTEPLEIALEEFD
                     RGVIPITVKRK"
     gene            2586..3773
                     /gene="eno"
                     /locus_tag="PLI76_10420"
     CDS             2586..3773
                     /gene="eno"
                     /locus_tag="PLI76_10420"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOI62489.1"
                     /translation="MTTIEQIILRTILDSRGNETVEAEIYTDCGFGRAAAPSGASTGT
                     HEARVRPPREAVEDARKNLIPSLIGEDAHDQITFDALLREYDATPNFSSIGANVAVAL
                     SLACAKAAASSLDIELFRYLGGAFATETPLPLGNVIGGGAHASGATSIQEFLVVPTGA
                     SGATEGVFVNAAVHRAVKKILQAQGKLSGKGDEGAWAPAISDIEAFDIIASAIDTVSD
                     ETNVEVRMGIDVAASELWDGERYRYKDAARTREDQIAYMGDLVDRYNLIYVEDPLFEE
                     DFDAFADLTELVGDRCLICGDDLFVTNVERITKGIETSAANCVLIKPNQIGTLTDTFE
                     AIHLARESGMETVMSHRSGETTDATIAHLATAFGCIFLKTGVVGGERIAKLNELIRIE
                     ELI"
BASE COUNT          840 a         1101 c         1224 g          626 t
ORIGIN      
        1 cacgacagca tccgcggcaa gggcggccgg agaggaagga gagtgtaatg gagattgcgt
       61 tttctcgact ggacgagaga gtcgcccgat tcaccataag cggcgtttcg acgtcgtttg
      121 caaacgtgct ccggcgagcg atggtcagcg aagtgccgac gctcgcaatc gaggatgtcc
      181 gcatatacga caacacgagc gtcatcttcg atgagatgct ggcacaccgg ctggggctcg
      241 tacccctccg gaccgacctt gcacgctacg ccgtccggag cgagtgctcc tgcggaggcg
      301 ccggttgccc ggtctgcacc gccacttaca cgctctccgt cgagggacca aagacggtct
      361 actcaagcga cctgatctcc caggaccccg atgccgcacc ggcggaagga ggtatcccca
      421 tcatcgatct cgggccggag cagaagatcg tgctcgaggc gcacgccatc gtcggcaccg
      481 ggaaggagca cgccaagtgg caggcgacga ccgcctgcgg ttacaagaac tacccggtga
      541 tcgccgtcga cgagcggtgt gacgggtgcg gcatgtgcat cgatgagtgt ccgcgcgatg
      601 tgctcgaggc ggggcagggc aaggtccggg tcgtcagcgg gcggcaggag ctctgttcgc
      661 tctgcaggct ctgcgaacgg gcgtgccttg ccggcggcat cggcaccgag ccggccatcc
      721 ggatcggcac cgaaccggag cgattcatct tcgtggtgga gagcgacgga tcgatgcccg
      781 cccgggagat cgtcgagaga gcgctccagt acattcagag atcatcagac gacctgatag
      841 atgtgataaa cgatatgacg ggagagggga ctgaatgaag acaacccaga ataaatccaa
      901 cccccggctg gccgccctca tcgtgacgct gaaagatgcg tcgcgtgtac acgaggcgaa
      961 gatctggcgt gagattgcgc ggaggctgga tgccccccgg aagaactacg ccgaggtgaa
     1021 cctcagcaag atcgaccggt acgcaaacga aggcgagacg atcctggtgc cgggcaaggt
     1081 gctcggcagc ggtgcgctca acctgccggt gaaggtcgcc gcgctggact tctcagaggc
     1141 cgcggtgagc aagatcaccg gcgccaacgg aacgtgcatg accatcgagg acctcctccg
     1201 ggacaacccg aaggggagcc gggtacggat cctcaggtga gacgaatggt aacagttatc
     1261 gatgcagacg gactgctgct cgggaggatg gcaagcatcg tcgcgcagcg cgcgctcgcc
     1321 ggcgaagaga ttgccctcgt gaacgtggag aaagcgatcg tctccggcaa ccgggcgcac
     1381 gtgctcgcgg cttactacac gaagcgcgac cgggggtccc gcgagggcgg cccgttcttc
     1441 ccgcgcaggc ctgaccacat cgtcaagcgc accatccgcg ggatgcttcc ctacaagcgc
     1501 gagcgcggca tcgcggcgtt caagcgggta aagacatacg tcggcgttcc catgcagttt
     1561 gccggtatgg agaccgaatc cctggaagcg gcccacatcg accgattgag cagcccgagg
     1621 tatataacgg tcggggcgat aagcaagaac ctcggagcaa agtactgatg aggtgacgga
     1681 atggcaaaga ttatcaatac aagcggtaag agaaagacgg ctatcgcccg ggcgaccctg
     1741 aaacccggca acggccgggt ccgcatcaac tccgtgcccc tggaggtcta cgggaccgag
     1801 ctgacgcgca tgaagatcgc cgaacccctg ctgctgatcc cgaacgcgct cgacggcgtc
     1861 gatgcggcga tcgacgtctc cggcggcggt gtggtgggac aggccgaggc ggtccggacg
     1921 gcgcttgcac gcggcatcgt ggagtggcac aacgaccccg cgatcaagga tgcgttcctt
     1981 gcgtacgacc ggacgctgct cgtgaacgac tcgcggcaga aggagaccaa gaaaccgcac
     2041 ggtccgggtg cacgggcacg gttccagaag tcctaccggt gaggaaaaga gatataaggg
     2101 atacaggaca atttatatga tacccgtacg atgttttaca tgcggtaaag tcatctctac
     2161 agcctggaag gagttcgaga ggcggcgaga tgcgggcgag gatccgaaac ggatcctcga
     2221 cgctctcggc ctggagcggt attgctgcag gcgaatgctg ctgacgcaca aggaagtagt
     2281 ggaggatctg aacccgtatc aatgaggggt cgtggggtag cctggactat cctatggcgt
     2341 tcgggatgct gtgacctgag ttcaaatctc agcgacccca ttttattacg aatgattttg
     2401 aggctgcacg atggaatcat atacccgata tgaacgagcg cggatcatag gggctcgtgc
     2461 tctgcagata tcgatgggtg caccggttct ggttaagaca ccaaataccg agccgcttga
     2521 gatcgcactt gaagagttcg atcgaggcgt gatccctata acggtgaaga gaaagtagtg
     2581 atctgatgac gaccatcgaa cagattatct taagaacgat cctggatagc cggggaaacg
     2641 agacggtcga ggccgagatt tacacggatt gcggttttgg gcgtgcagcg gcgccgagcg
     2701 gcgcgagcac ggggacccac gaagccaggg tgcggccgcc gcgcgaggct gtcgaggatg
     2761 cacggaaaaa cctgattcca tcgctcatcg gtgaggacgc tcacgatcag atcacgttcg
     2821 acgcactgct cagagagtac gatgcaacac cgaacttcag ctcgatcggc gcaaacgtcg
     2881 ccgtagcgct ctcgcttgcg tgcgcgaagg cggctgcatc gtctcttgac atcgaactct
     2941 tccggtacct gggcggcgca ttcgccaccg agacgcccct ccctctcggg aacgtcatcg
     3001 gcggcggcgc gcacgcatcg ggcgccacct ccatccagga gttcctggtg gttcccaccg
     3061 gtgcctccgg cgcgacggag ggcgtcttcg tgaacgccgc cgttcacagg gccgtaaaga
     3121 agatcctgca ggcgcagggc aaactctccg gcaaagggga tgaaggtgcc tgggcaccgg
     3181 ccatatccga catcgaggcg ttcgatatca tcgcttcggc gatcgatacc gtctctgatg
     3241 agacgaacgt cgaggtccgg atggggatcg acgtcgcggc aagcgaactc tgggacggcg
     3301 agcgttaccg ctacaaggat gccgcccgga cccgcgagga ccagatcgcc tacatgggcg
     3361 atctcgtcga ccgctacaac ctcatctacg tcgaagatcc cctctttgag gaggacttcg
     3421 acgcgttcgc cgacctgacc gaactggtcg gggaccgctg cctcatctgc ggcgacgacc
     3481 tcttcgtgac caacgtcgag cggatcacga aaggcatcga gaccagcgcg gcaaactgcg
     3541 tcctgataaa accaaaccag atcggcacgc tcaccgatac cttcgaggcg atccacctcg
     3601 cccgggagag cgggatggag acggtcatga gccaccggtc cggcgagacc accgacgcga
     3661 cgatagcgca ccttgcaacc gcatttgggt gcatattcct caagacaggc gttgtcggcg
     3721 gagaacgaat agcaaaactg aacgaactga ttcgcataga ggagctgatc taacttgaca
     3781 ggaaacgaac t
//
LOCUS       DAOSEE010000182         3788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_8525_length_3788_cov_229.677471, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000182 DAOSEE010000000
VERSION     DAOSEE010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8525_length_3788_cov_229.677471"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..513
                     /locus_tag="PLI76_10425"
     CDS             16..513
                     /locus_tag="PLI76_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOI62490.1"
                     /translation="MTAFECTLCGRCCMHAGGELIEIEKRLTSRDYLCRQKIVGGTFR
                     ARVEERFLDAFRDTTTRDAHPAWCPFLRPLPGERGKYVCTIHETRPLVCRSYICCTMR
                     IFAGDGREAGKVKGRRSLTTEDAALRRCWEESVVPLATDDDTAWKSDVAAVLERAGYR
                     VEVYE"
     gene            506..766
                     /locus_tag="PLI76_10430"
     CDS             506..766
                     /locus_tag="PLI76_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62491.1"
                     /translation="MSEVRRLLVARGRVQCTPHATVPVDRCGFCVHSARVVVKGREVP
                     SPARAYCSRCRDTSPVDMAKVEEIVCDDLSGEGFRSIANIIS"
     gene            complement(771..1109)
                     /locus_tag="PLI76_10435"
     CDS             complement(771..1109)
                     /locus_tag="PLI76_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein E"
                     /protein_id="HOI62492.1"
                     /translation="MAMRCSFALDPDLMEQIDQFARDRAYDRNEAILELIEAGLACQS
                     DGRTATVRQQRSFEELNRLMREIEEIKTVLTELRNEVRLVHHTIETDWNNEAKGVPFQ
                     TKHPWEFWRK"
     gene            complement(1287..1592)
                     /gene="yciH"
                     /locus_tag="PLI76_10440"
     CDS             complement(1287..1592)
                     /gene="yciH"
                     /locus_tag="PLI76_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HOI62493.1"
                     /translation="MNGGICPTCGLPKELCICEEVAKEQQRISVKINRRRYGKEVTVI
                     EGLDPYDIDLDDLSKFLKAKLACGGTVKDSSIELQGNHRERVKDLLAQKGYNMENIS"
     gene            complement(1635..3281)
                     /locus_tag="PLI76_10445"
     CDS             complement(1635..3281)
                     /locus_tag="PLI76_10445"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOI62494.1"
                     /translation="MELSPAARLLLERRYLLPGETPDALFARVAGAVGGPERSREFFS
                     LLSRLLFLPNSPTLMNAGTPAGQLSACFVLPIEDTLEGIFTSLAHMALIHRSGGGTGF
                     SFSRLRPRGDAVNGVVGAASGPVSFMRVFDAATGAVRQGGRRRGANMGVLAASHPDIE
                     EFVTAKRAGGLRNFNLSVGIDERFLRCLATGKDYDLTNPRDGSVSRSIDPASLWRLIA
                     ASAHASGEPGVLFLDEINRQSTTPHLGPIEATNPCGEQPLYPYESCNLGSVNLARCIK
                     NHDIDEDLLATVVRCGIDFLDAVIDANRFPLPVIREKTLATRKIGLGVMGFAEALIRL
                     GIPYESDEALRFAGKLMEGIEALARERSGELGAVKGSFPAIEGSVYTDDMRNATVTTI
                     APTGSLHLIAGTTSGIEPVFSFAYSRVIDGQQVAIVSDLVRELLPATAGGMDVAEHVR
                     RHGTVEGLPLADHTRDLFRTAVDIAPEHHVRMQAAFQRHVDNAVSKTVNLKETATAGE
                     IARIFSLARDLGCKGITVYRYRSRPDQVLSRGCDVCIVDD"
     gene            3340..>3788
                     /locus_tag="PLI76_10450"
     CDS             3340..>3788
                     /locus_tag="PLI76_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOI62495.1"
                     /translation="MSLIVLGTASHVGKSMTVTALCRALSRRGISVAPFKSQNMSLNS
                     YVTADGSEIGIAQAVQAFAAGVEPEADMNPVLLKPKGDRTSQVVLLGRPYKDVQIQDY
                     YTETDLLLSEAVSAFERLRERYGHVVVEGAGGAAEVNLYDRDIANIRL"
BASE COUNT          639 a         1184 c         1269 g          696 t
ORIGIN      
        1 agagagtcta cagggatgac ggcgtttgaa tgcacgctct gcgggaggtg ctgcatgcac
       61 gcgggcggcg agctcatcga gatcgagaag aggctcacga gccgggacta cctctgccgg
      121 cagaagatcg tcggcggcac gttccgcgcc cgggtcgagg agcggttcct cgatgcgttc
      181 agggatacca caacgagaga tgcgcacccc gcctggtgcc cgttcctgcg cccccttccc
      241 ggggagagag ggaagtacgt ctgcaccatc cacgagaccc ggcccctggt ctgccggtcc
      301 tacatctgct gcaccatgcg gatattcgcc ggcgacgggc gggaggccgg gaaggtgaag
      361 gggaggcgga gcctgaccac cgaggatgcc gccctccgcc ggtgctggga ggagtcggtc
      421 gtaccgctcg cgaccgacga cgataccgcc tggaagagcg atgtcgccgc ggtgctcgag
      481 cgtgcgggct accgggtcga ggtttatgag tgaggtccgg cgccttttag tcgcccgggg
      541 ccgggtgcag tgcacgccgc acgcgaccgt gcccgtcgac cggtgcgggt tctgtgtcca
      601 ctccgcccgc gtcgtggtaa aagggagaga ggtgccgtcg ccggcccggg cctactgcag
      661 ccggtgccgt gatacatctc ccgtcgatat ggcgaaggtc gaggagatcg tctgcgacga
      721 cctctccggg gaggggttcc ggagcatcgc aaacatcatc agctgacgga ctacttccgc
      781 cagaactccc aggggtgttt cgtctggaac gggacgccct tcgcctcgtt gttccagtcg
      841 gtctcgatgg tgtggtggac cagacggacc tcgttcctga gctcggtgag gaccgtcttg
      901 atctcctcga tctcgcgcat gagccggttc aactcttcaa acgagcgctg ctgccgcacg
      961 gtcgccgtcc tgccgtcgct ctggcaggca agaccggcct cgatcaactc cagtatagcc
     1021 tcgttccggt cgtatgcacg atctctcgca aactgatcga tctgctccat caggtcggga
     1081 tcgagtgcaa acgagcatct catcgccata ccctccattt ctccaggtga atccagataa
     1141 ggtttgctgg ttatacgcaa ccgattttct gaggcgcacc gcctgccggc cgcctcaccg
     1201 tccgggcgga cgcgaccgtt tccggcgcag tcgttccccg cccggcaaaa aaaacacccc
     1261 ggttctccgt ggagaaccgc ccggggtcaa ctgatatttt ccatattgta tcctttctgg
     1321 gcaagcaggt cctttacccg ctcgcgatgg ttgccctgca gctcgatcga ggagtccttg
     1381 accgtgccgc cacaggccag tttcgctttc aggaacttgg agaggtcatc gagatcgatg
     1441 tcgtagggat cgagcccctc gatgaccgtg acctctttcc cgtaccgccg cctgtttatc
     1501 tttacgctga ttctctgctg ttcctttgca acttcttcac agatacataa ctcttttggc
     1561 agtccgcagg tcggacatat cccaccgttc attgcatgta cctttcagct ctcgttatat
     1621 attatttggc atggtcaatc atcgacgata cagacgtcgc atccccgcga gagaacctgg
     1681 tccggccggc tccggtagcg gtagacggtg atgcccttgc acccgaggtc tcgggcgagg
     1741 gagaagatgc gggcgatctc gcccgcggtt gcggtctctt tgaggttcac cgtcttggag
     1801 acggcgttgt ccacgtgccg ctggaacgcg gcctgcatcc ggacgtggtg ttcgggcgct
     1861 atatcgacgg ccgtcctgaa gaggtcgcgg gtatggtccg caagggggag gccttcgacg
     1921 gtcccgtgcc ggcggacgtg ctccgcgacg tccatcccgc cggcggtcgc gggcaggagt
     1981 tcccggacga gatcgctcac gatagcgacc tgctgcccgt cgatcacccg gctgtaggca
     2041 aacgagaaga ccggttcgat gccgctcgtc gttccggcga tgaggtggag cgagcccgtg
     2101 ggggcgatgg tcgtgacggt ggcgttgcgc atatcgtcgg tgtagacgga cccctcgatc
     2161 gcgggaaacg agcccttcac cgccccgagc tcccctgagc gctcccgggc gagagcctcg
     2221 atcccctcca tcagcttccc ggcaaaccgg agcgcctcat ccgactcgta gggtatgccg
     2281 aggcgtatga gcgcctcggc aaaccccatg actccaaggc cgatctttct ggtggcaagg
     2341 gtcttttccc ggatcacggg cagggggaag cggttcgcgt cgatgaccgc gtcgaggaag
     2401 tcgatgccgc accggacgac cgtggcaagc aggtcttcgt cgatatcgtg gtttttgatg
     2461 caccgggcga ggttgacgct cccgaggttg cagctctcgt aggggtagag cggctgctcg
     2521 ccgcaggggt tggtcgcctc gatgggcccg aggtgcggag tggtgctctg ccggttgatc
     2581 tcgtcgagga agagcacccc gggctcgccg gaggcgtggg ccgacgcggc gatgaggcgc
     2641 cagagcgacg ccggatcgat gctccgcgag acgctgccgt cgcgggggtt cgtgaggtcg
     2701 tagtccttcc ccgtcgcaag gcaccggagg aaccgttcgt cgatgccgac cgagaggttg
     2761 aagttccgga gacctccggc ccgttttgcg gtgacgaact cctcgatatc ggggtgggat
     2821 gccgcgagca cgcccatgtt cgccccccgc cgccggccgc cctgccggac cgcgccggtc
     2881 gcggcgtcaa agacccgcat gaacgagacc ggcccgcttg cggcgcccac gaccccgttg
     2941 acggcatctc cgcgcggccg gagccgggag aacgagaacc cggtccctcc cccggaccgg
     3001 tggatgagcg ccatatgggc gaggctcgtg aatatccctt ccagggtatc ctcgatcggg
     3061 aggacaaaac aggccgagag ctgcccggcg ggggtgccgg cgttcatcag ggtcggggag
     3121 ttcgggagga agaggagccg ggagaggagc gagaagaatt cgcgggatct ctccgggccg
     3181 ccgacggctc cggcgacccg ggcaaagagg gcgtccggcg tctcgccggg gaggaggtag
     3241 cggcgctcga ggaggaggcg ggccgcgggg gagagctcca tacccgggat atggatcccc
     3301 gcccttaata atccgccgtg tccaaccctc ctgccggcaa tgtctcttat cgtccttgga
     3361 acagcatctc acgtgggaaa gagcatgacg gtgacggcgc tctgccgtgc gctctcccgc
     3421 cgcggtatat cggtcgcgcc gttcaagtcg cagaacatga gcctcaactc ctacgtcacg
     3481 gccgacggga gcgagatcgg tatcgcccag gcggtccagg ccttcgcggc cggagtcgag
     3541 cccgaagccg atatgaaccc ggtcctcctc aaaccgaagg gggaccggac gtcgcaggtg
     3601 gtgctgctcg gccggccgta taaggatgtg cagatacagg actactacac ggagaccgat
     3661 ctgctccttt cggaggccgt ctcggcgttt gaacggctgc gggagcgcta tgggcacgtg
     3721 gtggtcgagg gggccggggg agcggcggag gtgaacctct acgaccgcga catcgcaaac
     3781 ataaggct
//
LOCUS       DAOSEE010000183         3730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_8700_length_3730_cov_278.471293, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000183 DAOSEE010000000
VERSION     DAOSEE010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8700_length_3730_cov_278.471293"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="PLI76_10455"
     CDS             <1..351
                     /locus_tag="PLI76_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HOI62496.1"
                     /translation="ALEVICGGMARSHNLKAAALATAHGLGRTGGTDGHLLHELGGVV
                     TCAEAETVEELLDAIRARATVIIGRERPLVEKAVMGTAVLPHHLPHTVPILQARWEQN
                     LPRIKRFIKAHLDG"
     gene            complement(358..1791)
                     /gene="cls"
                     /locus_tag="PLI76_10460"
     CDS             complement(358..1791)
                     /gene="cls"
                     /locus_tag="PLI76_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOI62497.1"
                     /translation="MTFDILFVIIMVLNILFAVTIVFFERKNPTAAMAWLMVLFFLPP
                     IGFFLYLFFGQNYTRQRMFVVKREEDRRFLQEIFLAQHREFADTRYRFAIPEVDEFRE
                     AILLLLQNNRAFLTGKNRVDLYTRGEDKFDALFAAIREARHHIHLEYFILNNDELGRA
                     VVHALAEKALEGVEVRLLFDAMGTRAGGGTQKAFSELTDAGGKIGVFFPSVYRVNYRN
                     HRKIAVIDGTVGFIGGFNIGDDYLGKGPLGPWRDTAFRIAGQAVQMLQLRFFLDWHYV
                     TGGYPGPRPRYFPAPGDHGTTPIQIVSSGPDTRWSPIKEGYIKLINSARESVYIQTPY
                     FVPDESVTDALRIAALSGVDVRVMIPCKPDHPFVYWASLSFIGDLLDAGVRAYTYDNG
                     FLHAKTIVVDGKAGSVGSANWDVRSFRLNFEANAFFYDAAVGAELARAFKEDLALSTE
                     ITLESYRARSRRVRMKESVSRLFSPLG"
     gene            complement(1879..2418)
                     /locus_tag="PLI76_10465"
     CDS             complement(1879..2418)
                     /locus_tag="PLI76_10465"
                     /inference="COORDINATES: protein motif:HMM:NF018410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HOI62498.1"
                     /translation="MFLTGVLIWLHQAVKALAALLILVTLLPLVLGLVLGIPAARVLG
                     LISSTIVLQANAVFVGVGMGIHPAVVLLIMTLVEVGVVLAIYSICDAFALQSARVRGM
                     LERTEEKIQKVPLLARYGAVTLVVLPAMPIIGLYSSTVISWILGWNRMLSLIFITIGW
                     VAVTAFLLLVALGFVQVLF"
     gene            2620..3117
                     /locus_tag="PLI76_10470"
     CDS             2620..3117
                     /locus_tag="PLI76_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HOI62499.1"
                     /translation="MANTTMLIQGIVALALGVLLLTYPVGVLMILTIILGIYWLIDGI
                     VVLTNLYSDRTDKGWKVLVGVLGIIAGILVLAYPLYSAILLPTFLAIIIGVEGLIIGV
                     VHLVRGVTGAGFGSGVLGIVSIIFGLILIAHPFIAALALVWVLAIIAIAGGILAIAFA
                     LRKSA"
     gene            3335..3730
                     /locus_tag="PLI76_10475"
     CDS             3335..3730
                     /locus_tag="PLI76_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HOI62500.1"
                     /translation="MLDRMKESSGPVIGFRFDGKLSESDYTTLLIPELERAMKDHRNV
                     RVLLKIEGFRSWRPGEPWEAFRQWSGVESVDRVAVVGGERWREWMNRLPGLFVGFSGI
                     DVRYFPENRLRDAWGWLREGSVQIPLAAQ"
BASE COUNT          749 a         1156 c         1160 g          665 t
ORIGIN      
        1 gccctcgagg tgatctgcgg tggtatggcc cggtcccata acctgaaggc agccgctctt
       61 gccaccgccc acggcctcgg ccggaccggc gggaccgacg gccacctcct ccacgagctc
      121 ggcggggtcg tcacctgcgc ggaggccgaa accgttgaag aactcctcga cgccatccgg
      181 gcgagagcga ccgtcatcat cgggcgagag cggccgctcg tcgaaaaggc cgtgatgggg
      241 accgcagtcc tcccgcacca cctcccccac accgtcccca tcctccaggc acgctgggag
      301 cagaacctcc cccggatcaa gaggttcatc aaggcccacc tcgacgggtg aggtggctca
      361 cccgaggggc gagaagaggc gggagaccga ctccttcatc ctgacccggc gcgaccgcgc
      421 ccggtaactc tccagcgtga tctcggtgga gagagcgaga tcttccttga atgcccgtgc
      481 aagttcggcg ccgaccgccg catcgtagaa gaaggcgttc gcctcgaagt tcagccggaa
      541 actccgcaca tcccagttcg cgctcccgac cgatccggcc ttcccgtcga cgacgatcgt
      601 ctttgcgtgg agaaacccgt tgtcgtatgt gtaggcccgc accccggcgt cgagaaggtc
      661 gccgatgaac gagaggcttg cccagtagac gaaagggtgg tcgggtttgc aggggatcat
      721 gacgcggaca tcgaccccgg agagcgctgc aatccgtaga gcatcggtga cactctcgtc
      781 gggcacgaag tagggcgtct ggatgtagac cgactccctg gccgagttga tcagcttgat
      841 gtaaccctcc ttgatcgggc tccaccgggt atcggggccg ctcgagacga tctggatcgg
      901 cgtcgtcccg tggtcgcccg gggccgggaa gtagcgtggg cggggcccgg gatacccgcc
      961 ggtcacgtag tgccagtcaa ggaagaaccg gagctggagc atctggacgg cctgcccggc
     1021 gatccggaaa gccgtgtcgc gccagggccc gagcggaccc ttccccaggt aatcgtcccc
     1081 gatgttgaac ccgccgatga accccaccgt cccatcgatg accgcgatct ttcgatggtt
     1141 gcggtagttg acccggtaga ccgaggggaa gaagacccct atcttaccgc cggcatcggt
     1201 cagttctgag aacgccttct gcgttccgcc gccggcgcgg gttcccatcg cgtcgaagag
     1261 gaggcggacc tcgacaccct ccagcgcctt ctctgcaagg gcgtggacga ccgcccgccc
     1321 cagttcgtcg ttgttgagga tgaagtattc caggtggata tggtggcgcg cctcccgtat
     1381 cgcggcaaag agcgcgtcga acttatcttc accccgggtg tagagatcaa cccggttctt
     1441 cccggtcagg aacgcccggt tgttctggag aaggagaagg atcgcctcgc gaaactcatc
     1501 cacctcaggg atggcgaacc ggtagcgagt atcagcaaac tcacgatgct gcgcgagaaa
     1561 tatctcctgc aggaaccggc ggtcttcctc cctctttacg acgaacattc tctgccgggt
     1621 ataattctgc ccgaagaaga gatagaggaa gaaccctatc ggcggcagga agaagaggac
     1681 catcagccag gccatcgccg ccgtcgggtt cttccgctca aaaaagacga tggtgacggc
     1741 aaataggatg ttaagaacca ttataatgac aaatagtata tcaaaggtca tcctgttgtt
     1801 ctccccttgc cagagtcgag catgatcact ctatctctcc agggatatcc atctaccgaa
     1861 cggtatatcg ccggcccgtc agaagagcac ctgcacaaac ccgagcgcga cgagcaggag
     1921 gaaggccgtg acggcgaccc acccgatggt gatgaatatg agggacagca tcctgttcca
     1981 cccgaggatc cagctgatca ccgtgctcga gtagaggccg atgatcggca ttgccgggag
     2041 aacgacgagg gtcaccgcac cgtacctcgc aagaagcggc accttctgta tcttttcctc
     2101 cgtgcgctcc agcatacccc tgacccgtgc cgactggagg gcaaacgcat cacagatcga
     2161 gtaaatggca agcacgacgc cgacctcgac gagcgtcatg atcagaagaa ccacagcagg
     2221 gtgtatcccc atacccacgc cgacaaaaac agcgtttgcc tggagaacga tggtggatga
     2281 gatcagacca agtacccggg ccgccggtat gccgagaacc aggccgagaa cgagggggag
     2341 gagcgtaacg aggatgagca atgccgccag cgccttcaca gcctgatgaa gccagatcag
     2401 cacaccggtg aggaacatgc acgacgaccc tgccgggcag gggaaaggca ggtgtgcaca
     2461 tataacttcc tgtatcacga ctgcccggca gcatgcgccg gagcacccgg gcccgggcgt
     2521 tccggggaac ttccgagtca gtctctggcc gatctcatgg gagatctgtt gccgaagagg
     2581 tgcatattta taccaggatt agaccacctg aggtattata tggcaaacac aaccatgctg
     2641 atccagggca tcgtcgccct tgccctcggg gtcctgctcc tgacgtaccc cgtaggggtt
     2701 ctcatgattc ttaccataat cctcggcata tactggttga tcgatggaat agtagtcctc
     2761 acgaaccttt actcggacag aacggacaag gggtggaagg tgctcgtcgg cgtcctcggc
     2821 atcatcgccg gcatcctggt cctggcctat ccgctctaca gcgccatcct ccttccaacg
     2881 ttccttgcca tcatcatcgg tgtcgaaggc ctgatcatcg gggtggtcca tctcgtgcgg
     2941 ggagtcaccg gcgccggttt cggatccggg gttctcggta tcgtgagcat catcttcggg
     3001 ctcatcctga tcgcgcatcc gttcatcgcc gctctcgccc tggtatgggt tctcgcgatc
     3061 atcgccatcg ccggagggat actggcaatc gcctttgcac tccggaagtc cgcgtgaccg
     3121 gaccgcccgc cgtgcggagc atgcccgacc gggagccact ccgggcaccc ggacgatggg
     3181 ccggtacggc cctccgcggc cggggccaga ccggggcgaa cgggctccgg gttcctccgg
     3241 ggagagaggt ggaaccggta cgccggtgac acagctactc gctcccggga ggagtgccgc
     3301 agcctttttc tcttccgggt gcaaaagggc acccatgctt gaccggatga aggagagttc
     3361 gggacccgtg atcgggtttc ggttcgacgg aaaactgagc gagagtgact ataccaccct
     3421 cctgatcccg gagctcgaac gagcgatgaa agaccaccgg aacgtccggg tcctcttgaa
     3481 gatcgaaggg ttccggagct ggaggccggg agagccctgg gaggcgttca ggcagtggtc
     3541 cggggtggag agtgtcgatc gggtcgccgt cgtcggcggc gaacggtggc gggagtggat
     3601 gaatcgcctg ccggggctct ttgtcgggtt ctccggcatc gatgtccgct acttcccgga
     3661 gaaccgcctc cgtgacgcct ggggatggct ccgggaaggg tcggtccaga tccctctggc
     3721 ggcgcaatga
//
LOCUS       DAOSEE010000184         3705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_8781_length_3705_cov_288.245205, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000184 DAOSEE010000000
VERSION     DAOSEE010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8781_length_3705_cov_288.245205"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..1589)
                     /locus_tag="PLI76_10480"
     CDS             complement(162..1589)
                     /locus_tag="PLI76_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI62501.1"
                     /translation="MNQQPFVDRESELQFLTDRYRSPVPECLILYGRRRVGKTTLLAR
                     FLQTVPGFYFLASEEGTARNIQDFSHYAGEFLNDPDFERSRYPDWETVIKALVRHRNF
                     SPPPEKKVVIIIDEFPYLIARDRATPSLFQRVWDTVLSQEPVMLVISGSSVSTMETEV
                     LGYTSPLYGRRTGQWQLEPLSYPYLRRFLPYDEEDLIMTWCVIGGIPAYLRLFRPDRT
                     FWENVEEQILRKGAYLYSEAEILMQYEFREAGNYMAILRALAAGYTALAPLCQETGLD
                     KGLVSKYLAVLSRMHLINDEVPVTAHAGYRRRHYRLTDPYLTFWFRFVYPRRAETEMG
                     QSREVLASIREGIAPYCGQMFEQLSHDLICRGILFGSSYSRLGRWWQKEAEIDLVGLN
                     EVSGDVLFCECKWSAVNHRQARGILAALREKAGEVRWQNESRNERFALVAKSIEGKED
                     LREDGYLVADLEDIARLSREYLAGR"
     gene            complement(1839..2201)
                     /locus_tag="PLI76_10485"
     CDS             complement(1839..2201)
                     /locus_tag="PLI76_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62502.1"
                     /translation="MQTIEISDKTILAHRHGKESINKTIERKFKQKRPEKESKALQEI
                     DEIRKGKFYTRTQVEESINGFSGYALRPANLSFDPRLHHTRVWQKSLHAFPSGRARRI
                     RKTSISTWSTCTARGQGW"
     gene            2656..2832
                     /locus_tag="PLI76_10490"
     CDS             2656..2832
                     /locus_tag="PLI76_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62503.1"
                     /translation="MVAASDLSPGLKDIIDEFEENWNDFALQDESTLSGGALNLRRKL
                     LSLVVNYPLLSSGA"
     gene            3443..3589
                     /locus_tag="PLI76_10495"
     CDS             3443..3589
                     /locus_tag="PLI76_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62504.1"
                     /translation="MAKPIDLGLTLEGEDARCFHEYMERPTYTDAARELIRSAAREAQ
                     NRRL"
BASE COUNT          761 a         1065 c         1037 g          842 t
ORIGIN      
        1 cggcgctcgc cgtcgcatct ctcttctgcg tttgcactcc cgcggaggcg gtattgactc
       61 cgatgccgcg ggcaggccgg agctcatcgt cgcggtgaag atcgaacgaa gagcagcatg
      121 ggaacgatcg cgcgaacatg cagcgagagc gatcccctct ctcaccttcc cgccaggtac
      181 tcccgcgaaa gccgggcgat atcctcgaga tcagcgacca gatacccgtc ctctctcaga
      241 tcttctttgc cttctatgct ctttgctacc agggcgaagc gttcgttcct gctctcgttt
      301 tgccatcgca cctcaccggc cttctcccgg agtgccgcaa ggatgccgcg ggcctggcgg
      361 tggttcacgg cagaccactt gcattcgcag aagaggacat ccccggagac ctcatttaag
      421 cctacgagat cgatctccgc ctctttctgc caccaccgcc ccagtcgcga atacgaggaa
      481 ccgaacagga tgccccggca aatgagatcg tgagagagtt gttcgaacat ctgcccgcaa
      541 tagggtgcga tcccctccct gatagaggcg agcacctctc tactctgccc catctcggtc
      601 tctgccctcc gggggtacac aaaacggaac cagaacgtga gatagggatc ggtcagccgg
      661 tagtgccttc tccggtagcc ggcatgggcg gtcaccggca cttcatcgtt gatcaggtgc
      721 atacgcgaaa ggacggcaag gtatttggag accaggcctt tatcaagccc tgtctcctga
      781 cagaggggtg ccagggcggt ataccccgct gcgagagcgc ggaggatggc catgtagttc
      841 cctgcttcgc ggaactcgta ttgcatcagg atctcggctt cagagtaaag gtatgcacct
      901 ttgcggagga tctgctcctc cacgttctcc cagaatgttc ggtcggggcg aaacaggcgg
      961 agataggcgg gaattccgcc gatcacgcac catgtcataa tgagatcttc ctcatcgtag
     1021 gggaggaacc gacggaggta ggggtaggag agaggctcga gctgccactg ccccgtcctt
     1081 ctgccgtaga gcgggcttgt gtaccccagc acctcagtct ccatcgtgct gaccgatgaa
     1141 cccgatatga cgagcatcac cggttcctgc gagagcaccg tatcccacac cctctggaag
     1201 agggagggcg tagcccggtc gcgagcgatg aggtagggga actcgtcgat gatgatcacc
     1261 accttctttt ccggaggagg ggagaagttc cggtgcctga ccagtgcctt aatgacggtc
     1321 tcccagtcgg gatatctact ccgctcgaaa tccggatcgt tcaggaattc ccccgcatag
     1381 tgggagaagt cctggatgtt ccttgccgtc ccttcttctg aggccaggaa gtaaaagccc
     1441 gggaccgtct gcaggaatcg ggcaagaagc gtcgtctttc ccacgcgccg cctcccgtag
     1501 aggatcaggc attccgggac cggggagcga tagcggtcgg tgagaaactg gagctcgctc
     1561 tcccggtcta caaagggttg ttgattcata agtatactac tttatagtca acaatacact
     1621 taaggcgatc ggtacgtgcc gggcatcccc ttaccgggat agatggtcgt agccgggcgg
     1681 gatgcgggcg caatcccctg ttttcgcggc gacgcccggg aatcaccccc gggcggaggc
     1741 caagccgccg cggtttgacc tggagcctgc cggaaaccgg agattccggc tggtcccgag
     1801 atgatcgaca tcacgcatgt attgcgcatc tacgcacatc accatccctg tccgcgagca
     1861 gtgcaggtgc tccatgtaga gatcgacgtc ttccgtatcc gccttgccct tcccgaagga
     1921 aatgcatgca ggctcttctg ccacaccctt gtgtggtgca ggcgtgggtc aaaagaaagg
     1981 tttgccgggc gaagtgcgta tccggagaaa ccgtttatgc tctcttcaac ttgcgttctg
     2041 gtatagaatt tacctttcct tatttcgtct atttcctgta gcgctttact ctccttttcc
     2101 ggccttttct gcttaaattt acgctctatt gttttgttaa ttgattcttt tccatgccgg
     2161 tgtgcaagaa tcgttttatc cgagatctcg atcgtctgca tgtacgtggt gtctgccatc
     2221 cagggggata tacgttgtgc agttggtgca aaattcctgc ttcccgggca attttcgtcc
     2281 attcgagtag ggacgagccg ggaaacgccc atgtaggctg catattccgg ccgatctctc
     2341 cctgagatgg tttgcagcgc tgatacccgg gatcgaacct gatatgtatt tccaggtggt
     2401 acacagtggt atgtccgaga cgacgtcgat taagatctcg gaagaggtgg aaagatcgac
     2461 tcaatcgcct caagatacat cctcgtgaga cctatagcga tctcctctcg cggttgacct
     2521 ctcaggcgca aacggaacga tttccatggc ataccccact catctacatc aggattcgag
     2581 gggtcatccg ggagctcaag gcaccctatc gcgatctcca tcgagaggga tgtacatctt
     2641 ctagagtgcc acctgttggt cgccgcctcc gatctctccc cgggcctgaa agatattatc
     2701 gatgagtttg aggagaactg gaacgacttt gccctgcagg atgagagtac tctatccggc
     2761 ggtgctctca acctgaggag gaaacttctc tcacttgtag tcaactaccc cctcctctcg
     2821 tcgggggcat gataccatga atggtatcac gctctttaca ggcttgcgat tccacctttc
     2881 ccccgatgtc ggggaagcgg acgcgagtag gccggttgac ggcggccctg gcagcacgat
     2941 cccgtgctta ccgcataata ctgttttgcg ccaatatgta gccgtgatca gcaggagaag
     3001 ttcctgtttc gtgcctttgc tggcgcaaac cagattgcta tggatcctca tccggcataa
     3061 aggatacgta tgcggggtga aagtgtattg ggttccgcag agggccgatt cctccccctc
     3121 ctctcgtcgg ggggctcctc ggcccacgat cctgaaggag acctgatcgc gtcccggaga
     3181 tccgggtcgc catatgccgg ctcatgaaag gtaggtttga tgccgggata tcaaagcata
     3241 tccagccgtt gttccgagat tattttcgcg cacgtcgccg ggcagcatgt cggtccagga
     3301 tggcccgatc gtcacccgct catcgactgc aggcataacg gcttcctcct gcctataggg
     3361 gaacacaggg gttaacctga cgccagtgga aagatatttg agatggtgcg atcattatat
     3421 catattcaga ggcgaagcga ggatggcaaa accaatcgac cttgggctta cccttgaggg
     3481 agaagacgcc cggtgtttcc acgagtatat ggagaggccc acctataccg acgccgcccg
     3541 ggaactgatc cgatccgcag cacgcgaagc gcaaaaccgg cgcctgtgaa cataacatgc
     3601 ccctcccgga gatccgtttt ttgccgcttt cccgggatct ggatgtatca actttcagtt
     3661 gtggccacga cgatctagat gaattcctga tcgaggactc gatgg
//
LOCUS       DAOSEE010000185         3682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_8851_length_3682_cov_176.443893, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000185 DAOSEE010000000
VERSION     DAOSEE010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8851_length_3682_cov_176.443893"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1691
                     /locus_tag="PLI76_10500"
     CDS             <1..1691
                     /locus_tag="PLI76_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HOI62505.1"
                     /translation="DCIVPSNVREGYLARLVLRRTLRMMNDLSMDEDLADLVEAQMQA
                     VGDFSQDVDVVREIVASEEARYAGTLERGTRIVQKIARNYRAKSSRVPLAEVITLYDS
                     HGIPPEMVKEVAAAEGAVVEIPDNFYSLIADIHSEAQKEAEGADPLAPYRERAATLPP
                     TKKLYYELPCEVEFEAMVLDYFDGMAVLDQTLFYPEGGGQPSDTGTLVTSESMVRVEE
                     AVKIGEVILHRVTGGPLKRGDRVKGMVDEERRLSLMRHHTATHVLLHAAKKVLGAHVH
                     QAGAQKGSETSRLDVRHYRHITPDELRRIEIEANRMVMADLPVYICIEERTKAEQKYG
                     FDLYQGGVPPGREIRTVQVGAEVQACAGTHVRATGEIGPIRVIGVEHIQDGVERLVFA
                     AGLAAVYAIQRMADILGASADVVSVQPENLPATVTRFFSEWKEQKKEIERLRKSAVDL
                     RMQHLAGEVVDGVRVVVGQVDAAPKELVALATSVADEGGVALFVSVDGNVKVVATSGV
                     PAVNAADIVREVCSIIGGKGGGKPTLAQGAGSDVARLGQALEHGRSRILEALHG"
     gene            1684..>2066
                     /locus_tag="PLI76_10505"
     CDS             1684..>2066
                     /locus_tag="PLI76_10505"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOI62506.1"
                     /translation="MVDGVVVIQPGDERAQRIARAMASQTANAIIQAFGSGPLTSSEV
                     ARSMAIPITTATYHIDNLLNAGLIEVMETRWSEKGREVKVYGLANQVVIIAPPESDLR
                     SVLKKYATLFGIVVLASVGLWGILPA"
     assembly_gap    2067..2166
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2167..2393
                     /locus_tag="PLI76_10510"
     CDS             <2167..2393
                     /locus_tag="PLI76_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62507.1"
                     /translation="LPAPQDGMMPKVAGGGEEITAFRAPLENAAGAPPVHDLVMAFFF
                     GACAVLFALLAYEVYYWWRTMPRVEKEQGG"
     gene            2778..>3682
                     /locus_tag="PLI76_10515"
     CDS             2778..>3682
                     /locus_tag="PLI76_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62508.1"
                     /translation="MPSPEVVPRLIRQIYQVRPDLKLPEAAIHAVLFEVRRALPRDNP
                     GRERLPFYWYTHGPYSEIVRSSIENLERRGILREETKAGKTLLVLDARPAAVGCVCED
                     ELWRIVREIDPLEAFINRIYRDHAPYEFMPRYRVDFIASLEGYIASRPEGTLDQGSFC
                     GIDCLEDALYACEAVLVEEPLFEGFNDEFTAYVSGAGKAFDIARTGESAAYPAIRATC
                     AVARDVWCVFAGGVRILSRGHDDYYDGRLERWEQEYRKAFSALAPKVRAYNRYIRESA
                     RRTPHGEPDERTTRILSSLIEGYLPG"
BASE COUNT          620 a         1075 c         1283 g          604 t
ORIGIN      
        1 gcgactgcat cgtcccctcg aacgtccgcg aggggtacct cgcccggctg gtgctgcgcc
       61 ggacgctccg gatgatgaac gatctctcga tggacgagga tctcgccgac ctcgtggagg
      121 cgcagatgca ggccgtcggg gacttcagcc aggacgtcga tgtggtcagg gagatcgtgg
      181 caagcgagga ggcgcggtat gcgggcacgc tcgagcgcgg gacccggatc gtccagaaga
      241 tcgcccggaa ctacagggcg aagagctccc gcgtgcccct cgcggaggtc atcaccctgt
      301 acgactcgca cggcataccg cccgagatgg tgaaggaggt cgcggccgcc gagggcgccg
      361 tcgtggagat ccccgacaac ttctactcgc tgatagccga catacactcg gaggcgcaga
      421 aggaggcgga aggggcggat ccgctcgccc cctaccgcga gcgggcggca acgctccccc
      481 cgacgaagaa actctactac gagctcccct gcgaggtcga gttcgaggcg atggtgctcg
      541 actacttcga cggcatggcg gtgctggacc agaccctctt ctacccggag gggggcggcc
      601 agccgtccga taccggcacc ctggtgacgt cggagagcat ggtgcgggtg gaggaggccg
      661 tgaagatcgg ggaggtgatc ctccaccggg tgacgggagg ccccctgaag cgcggcgacc
      721 gggtgaaggg gatggtggac gaggagcggc ggttgtcgtt gatgcgccac catacggcga
      781 cccacgtcct cctgcacgcg gcaaagaagg tgctcggcgc ccacgtgcac caggcgggcg
      841 cccagaaggg gagcgagacc tcccgcctgg acgtccggca ttaccggcac atcacgcccg
      901 acgaactccg gcggatcgag atcgaggcga accggatggt gatggcggac ctgccggtct
      961 acatctgcat cgaggagcgg acgaaggccg agcagaagta cgggttcgac ctctaccagg
     1021 gcggggtgcc gccgggccgg gagatcagga cggtgcaggt cggcgccgag gtgcaggcgt
     1081 gtgcggggac gcacgtccgg gcaacgggtg agatcgggcc gatccgggtc atcggcgtcg
     1141 agcatatcca ggacggcgtc gagcggctgg tgtttgcggc cgggcttgcc gccgtctacg
     1201 cgatccagcg gatggcggat atccttggtg cctccgccga cgtggtgagc gtccagcccg
     1261 agaacctgcc ggcgacggta acgcgcttct tctcggagtg gaaggagcag aagaaggaga
     1321 tcgagcgtct ccggaagagc gcggtggacc tccgcatgca gcaccttgcg ggcgaggtgg
     1381 tggatggggt ccgggtcgtc gtcggtcagg tggatgcggc tcctaaggag ttagtcgcgc
     1441 ttgcgacgag cgtcgccgac gaaggcgggg tggcgctctt tgtatcggtc gacggaaacg
     1501 tgaaggtggt ggcgacctca ggcgtgccgg cggtgaacgc cgccgatatc gtgcgggagg
     1561 tctgcagcat tatcggcggg aagggcgggg gcaagccgac ccttgcgcag ggcgcaggat
     1621 cggacgtcgc ccggctcggc caggcgctcg agcacgggcg cagccgtatt ctcgaagcac
     1681 tccatggttg acggtgtcgt agttatccag ccgggcgacg agcgggctca acggatcgcc
     1741 cgggcgatgg cgagccagac ggcgaacgcg atcattcagg ccttcggcag cgggccgctg
     1801 acgtcgtcgg aggtcgcccg gtcgatggcg atcccgatca ccaccgccac ctaccatatc
     1861 gataacctcc tgaacgccgg tctcatcgag gtgatggaga cccgctggag cgagaagggg
     1921 cgcgaggtga aggtctacgg cctggcaaac caggtcgtca tcatagcgcc gccggagagc
     1981 gacctccggt cggtgttgaa gaagtacgcg acgctctttg gcatcgtcgt cctcgcgagc
     2041 gtcgggctct ggggtatcct cccggcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnntgct gccggctccg caggacggta tgatgccgaa ggttgccggg ggcggggagg
     2221 agatcaccgc gttcagggcg cccctggaga acgccgccgg ggcgccgccg gtccacgacc
     2281 tggtgatggc gttcttcttc ggggcctgtg cggtgctgtt cgctctgctg gcctacgagg
     2341 tctattactg gtggcggact atgccgcggg tagaaaaaga gcagggtggg taacagaggg
     2401 ccggggatgg ggcggcaggg gtctttactt tcgccccctg gagacccggg cttcctctgg
     2461 ttttccactc cccgcttcaa cttttgcccc gcacttgggg caccgcgtct tcttcgggtt
     2521 gatctctgca ccgcaactgt agcacttggg catggcgtat caccacacta tgcctgatcg
     2581 ggttacgata ccataaggct atctcatggt gttctcgggc aggtgtcgcg cgggggccgg
     2641 aacacccggg gaatgggcgg gaaatgcccc gggcggggtt caactccatc ggcgccggcc
     2701 ggccgggtat ttatgggggt gcgcgggggc ggggaggaga aatccctata acgccgcatc
     2761 gcatacagga gagtacgatg ccctcgcccg aagtcgtccc ccggctcatc cggcagatct
     2821 accaggtgcg gccggatctc aaattgccgg aggcggccat ccatgcggtc ctcttcgagg
     2881 tgaggagagc cctcccccgg gataaccccg ggagagaacg ccttcccttc tattggtata
     2941 cccacggccc ctattcggag atcgtcagat cctcgatcga gaacctggag cgccgcggca
     3001 tcctccggga ggagacaaag gccggcaaaa cccttctcgt gctggatgct cgtcccgccg
     3061 ccgtgggctg tgtgtgcgag gacgaactct ggcgtatcgt cagggagatc gatccccttg
     3121 aggcgttcat caaccgtatc taccgtgacc acgcaccgta tgagttcatg ccccgctatc
     3181 gggtggattt tatcgcctcg ctcgaggggt acatcgcatc gcgcccggaa ggcacgctcg
     3241 accagggatc gttctgtggg atcgactgcc tggaagacgc tctctacgcg tgtgaggccg
     3301 tgctcgtgga agaacccctc ttcgaggggt tcaacgacga gtttaccgcg tacgtctcgg
     3361 gggccgggaa ggcttttgat attgccagaa cgggcgaatc ggccgcgtac ccggccatca
     3421 gggcgacatg tgcggtcgcc cgggatgtct ggtgcgtgtt tgccggcggg gtgcgcatac
     3481 tgagcagggg acacgacgac tattacgacg gaaggcttga gcggtgggag caggagtacc
     3541 ggaaagcctt ttctgccctg gccccgaagg tgcgtgcgta taaccggtat atccgggagt
     3601 ctgcccgtcg caccccccac ggggaaccgg acgaacgcac aacaaggatc ctctcctcgc
     3661 tgattgaggg gtacctgccc gg
//
LOCUS       DAOSEE010000186         3646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_8952_length_3646_cov_194.429685, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000186 DAOSEE010000000
VERSION     DAOSEE010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8952_length_3646_cov_194.429685"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="PLI76_10520"
     CDS             <1..1140
                     /locus_tag="PLI76_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HOI62509.1"
                     /translation="ENPLWPCAMTGAVACLAGFEDLAVVVHGSSGCYFYPASLVGVPV
                     HGTFLIENEIIFGTESRLAEVIHDLEGRYSRIAVVNTCVPAIMGEDIGALARDGRVLI
                     VDSPGFIGDLEAGYRRALDALAPEVDEGIAGVNIDGICRTDPFCRGNAIEARRLLDLA
                     GASVGTTFCLDRFDAAHRAAPFTVGTNPDLASGVGTACGSLLGLDAVRETFERLGAVI
                     GGIDTRPVAEEVAWADERIRKACEKYLRRFDPPRVAIAAQSAYAAFAADLLDRYLGAD
                     IACVAARNDPGEGGAAAVRTTDFAVLQERMTGAEPDLVIGSSYERAMAPGAAFVGLTP
                     PLRDRVLLSSRALAGVEGALRLMDEVLNACTSRNRRVGSDYTDGF"
     gene            1224..1592
                     /locus_tag="PLI76_10525"
     CDS             1224..1592
                     /locus_tag="PLI76_10525"
                     /inference="COORDINATES: protein motif:HMM:NF025903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HOI62510.1"
                     /translation="MPLVKIEIRTGKPIEYRQLLIESLRRAFMDALQTPEAALWIRVC
                     ERKEENFPMPPGRSADAVLIEASLMPGRGPRAKRALYEAIVGHLARDAGIRPDDVCIV
                     LYEPPIENWAEHGEAFAPSR"
     gene            1695..1904
                     /locus_tag="PLI76_10530"
     CDS             1695..1904
                     /locus_tag="PLI76_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62511.1"
                     /translation="MTHYGGRAVHGMLLERMFDILDEDQVQQLMIRMIESSIKAKQQH
                     IDMMQYKIETYKMARDMLQAEMKNK"
     gene            1988..2452
                     /locus_tag="PLI76_10535"
     CDS             1988..2452
                     /locus_tag="PLI76_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOI62512.1"
                     /translation="MDLIIREYREEDFLSVSRLERENSPGDCKPEIFIRQAGVLFAGT
                     FLVAEVEGEVVGYTIGALVQHRQTTGWIVRLVVSGEHRRRGFGERLITAVAGSLRERG
                     AEEVYLSVAPSNHAARALYEKHGFREADFCPAYFGEGADRSILRRDLTAGKA"
     gene            2478..3062
                     /locus_tag="PLI76_10540"
     CDS             2478..3062
                     /locus_tag="PLI76_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOI62513.1"
                     /translation="MSFTPIRIGEVFRPTPAFRPYLYASLVLAVVFLVLPWLVPIILL
                     AWGIVAIAFALPTLAAIVFVIYWIPRYCESIVYRLTVTEITWQRGVWFQQTGIVPYNR
                     ITNVDIAQGPLMRLFAFSAVRVQTAGYSAQAQAEIVLNGIADPKDLQEKIMDFVRRTG
                     PVAVEGGPEEVPAAGAIVEELRAIRRLLESREGR"
     gene            complement(3161..>3646)
                     /locus_tag="PLI76_10545"
     CDS             complement(3161..>3646)
                     /locus_tag="PLI76_10545"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOI62514.1"
                     /translation="ECQSMFKRILFPTDFSAPSMKVLDYIPALQEVGTREVVLVHVID
                     SKDITLIASGGQGFLGTVSDRETETQRRLREEIEHQIIDARRSLEKRGLKVTVRTPVG
                     NPEKEILSAADAEEASLIVIGSHGRSNIRDRLLGSVSEYVIKNARQPVLVIKRDTAGA
                     K"
BASE COUNT          605 a         1169 c         1203 g          669 t
ORIGIN      
        1 gagaaccccc tctggccctg tgcgatgacc ggggcggtcg cctgccttgc ggggttcgag
       61 gacctcgccg tcgtcgtcca cggctcgagc gggtgctact tctaccccgc atccctggtc
      121 ggggttcccg tccacgggac gttcctcatc gagaacgaga tcatcttcgg gacggagtcc
      181 cgccttgcgg aggtgatcca cgaccttgag gggcgctact cgcggatagc cgtcgtgaac
      241 acctgcgtgc ccgcgatcat gggcgaggat atcggcgccc tcgcccgcga cggaagggtg
      301 ctgatcgtcg acagcccggg gttcatcggc gacctcgagg ccggctaccg ccgtgcgctc
      361 gatgcgctcg cgcccgaggt ggacgagggc atcgccggcg tcaacatcga cggcatctgc
      421 cgcaccgacc ctttctgccg ggggaacgcc atcgaggcgc gacgcctgct cgaccttgcc
      481 ggcgcctcag tcggcaccac cttctgcctg gaccgcttcg atgcggccca ccgtgcggcg
      541 cccttcaccg tcgggacgaa ccccgatctt gcaagcggcg tcggaaccgc ctgcggctcg
      601 ctcctcgggc ttgatgccgt cagggagacc ttcgagaggc ttggtgccgt tatcggcggg
      661 atcgataccc ggccggtcgc agaggaggtc gcgtgggccg atgagcggat ccggaaggcc
      721 tgtgagaagt acctccgcag gttcgaccct ccccgggtcg cgatcgccgc ccagagcgcg
      781 tatgccgcct ttgccgccga tctcctcgac cgctacctcg gcgccgacat cgcctgcgtc
      841 gcggcccgaa acgatccggg ggaggggggc gccgccgctg tccgcacgac cgactttgcg
      901 gttctccagg agaggatgac gggcgccgag ccggacctgg tcatcggctc ctcctacgag
      961 cgggcgatgg ctccgggtgc cgcgttcgtc ggcctgacgc cgcctctcag ggatagggtt
     1021 ctcctctcat cccgcgcgct ggccggggtc gagggggcgc tccggctgat ggacgaggtg
     1081 ctcaacgcct gcaccagccg caaccggcgg gtggggagcg attacacgga cggcttctaa
     1141 ggaaccgtta atacccgggg tgccagagca ctcatgccgg agaacgggaa tctctcccgg
     1201 atacccggct ggagctggcc cccatgcccc ttgtaaagat cgagatccgc accggaaaac
     1261 ccatcgaata ccggcaattg ctcatcgaaa gccttcgcag ggctttcatg gatgcactgc
     1321 agacaccgga ggccgccctc tggatccggg tctgcgagcg aaaagaggag aactttccca
     1381 tgccccccgg ccgatccgcc gatgcggtcc tgatcgaggc ctccctcatg ccggggcgtg
     1441 gtccccgtgc gaagagggcg ctctatgagg cgatcgtcgg gcaccttgcc cgcgatgcgg
     1501 gcatcaggcc ggacgatgtc tgcatcgtcc tctacgaacc gcccatcgag aactgggccg
     1561 agcacggtga agcgttcgcc ccatcccggt gaatccgccg gggtacctgc acccggcaca
     1621 aggtatttat cacccgccgg gcacagggtg ccggcctgtc cgtatgggca gggccgacac
     1681 aaggggggta tgatatgacg cactatggcg ggcgcgctgt tcacggtatg ctccttgaga
     1741 ggatgttcga tatcctcgac gaggatcagg tgcagcagtt gatgatccgg atgatcgagt
     1801 ccagtatcaa ggcaaaacag cagcatatcg atatgatgca gtacaaaatc gagacgtaca
     1861 agatggcgcg cgatatgctc caggcagaga tgaaaaataa gtgagccctt tgccccgggg
     1921 gcgctccggc gatcgagcgg cccggggctc ccttctcttt ttctcccttc gcgccgaaca
     1981 taccggtatg gacctgatca tcagggagta tcgcgaagag gattttctct cggtctctcg
     2041 cctcgagcgg gagaacagcc cgggtgactg taagcccgag atcttcatcc ggcaggcggg
     2101 cgttctcttc gccgggactt tcctcgtcgc ggaggtcgag ggagaggtcg tcggctacac
     2161 catcggggcc ctcgtgcagc accggcaaac gacggggtgg atcgtccggc tggtggtctc
     2221 gggggagcac cgccgccggg ggtttggaga gcggctcatc accgccgtcg ccgggagcct
     2281 ccgggagagg ggggcggagg aggtctacct ctcggttgca ccgtcaaacc atgccgcccg
     2341 ggcgctctac gagaagcatg gttttagaga ggcggatttc tgccccgcat acttcggtga
     2401 gggggccgat cgctccatcc tccggagaga cctcacggca gggaaggctt aatccttcca
     2461 ggttgatatg tgatttcatg tcgttcaccc cgatccgtat cggcgaggtc ttcaggccga
     2521 caccggcgtt ccggccgtac ctctacgcat cgctggtcct cgccgtcgtc tttctcgtcc
     2581 tcccctggct ggttcctatc atcctgctgg cgtggggcat cgtcgctatc gccttcgctc
     2641 tgcccaccct tgccgccatc gtattcgtca tctactggat accccgctac tgcgagagca
     2701 tcgtctaccg gctcaccgtc accgagatca cctggcagcg gggagtctgg ttccagcaga
     2761 cgggtatcgt cccctacaac cggatcacga acgtcgatat cgcccagggc cccctgatgc
     2821 ggctctttgc tttctcggca gtccgggtgc agaccgccgg ctactcggct caggctcagg
     2881 cggagatcgt cctcaacggg atagcggatc caaaggatct ccaggagaag atcatggact
     2941 tcgtccggag gacgggcccg gtcgccgtcg agggcgggcc ggaagaggtg ccggccgcag
     3001 gtgcgatcgt ggaggagctg cgggcgatcc ggaggctgct cgagagccgt gaggggcggt
     3061 aggagaggtg ccggccggga aatatggatg tgggtgcggg ctcacctgcg ggcggtgcgg
     3121 cccgcccgat ggcgtgggcg ggccaggaga aagaataacc ttatttcgcg ccggccgtat
     3181 cccgcttgat gaccaggacc ggctggcggg cgttcttgat aacgtactcc gagaccgacc
     3241 cgagcaggcg gtcgcggatg ttcgaccggc cgtgggaacc gatgacgatg agcgaggcct
     3301 cttccgcatc cgccgcggac aggatctcct tctcagggtt gccgacgggt gtccggacgg
     3361 tcactttcag gccccgtttc tcaagcgatc gccgggcatc gatgatctgg tgttcgatct
     3421 cctcccggag ccggcgctgg gtctcggtct cacggtctga tacggtcccg aggaacccct
     3481 gcccgcctga agcgatcagc gtgatgtctt ttgagtcgat gacgtggacg agaaccacct
     3541 cccgggttcc cacctcctgc agcgcaggga tgtagtccag caccttcatg gagggcgctg
     3601 aaaagtccgt tggaaacagg atgcgtttga acatgctctg acactc
//
LOCUS       DAOSEE010000187         3475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_9554_length_3475_cov_189.498246, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000187 DAOSEE010000000
VERSION     DAOSEE010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9554_length_3475_cov_189.498246"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            274..3417
                     /locus_tag="PLI76_10550"
     CDS             274..3417
                     /locus_tag="PLI76_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOI62515.1"
                     /translation="MSQPHPPSSGSLAGPSILDALTEGVLLIDREQRVAWINRPLERL
                     LGIDRDPIHGSNAGEFVRRHLGPCIIDRACADRVAASLSMSQSSIEVTCTFRSPGGRV
                     IISDVILQDEPFRGMRLVRFQGIAGQKQAEDALRESDETYRQIFASMGVGFAFYEMVR
                     DSAGRPVDYLILDIDPAYEHIMGAHRDRIVGQRFTEIYPGIEPLWIERYDKVLRTQKP
                     ARFEEYNPKSGRWYDIDAYPMREDDRFAVIFTEITGWKQAEEALQESEERYRTLFESA
                     SDALFIHDRNGKILDVNRAAVERLGYSREELLAICLTDIVLPEFAALVPDQTRAVVER
                     GRMIVETAHLARDGRRIPVETSSQVIPYRKGTAILAVSRDITRRKRSEEVLRHQAEMF
                     ERLIDTIPVMIVIFDPELRQFRFNRAFRETLGWTEEDIAGRDLMTVVYPDPDYRAKAA
                     AFMQSLEPGWREWKVTAKDGSIVESSWANISLADTTRIGIGIDLRERKRAEEALRASE
                     KRFRLLSENASDIVLVFDEQGIISYASPSVRPIGGYAPEDLIGRCIFEFVHPDDLPPA
                     RDALQRAAAHPGRQVTLEVRVRHSGEDWLYLDLTVNNLLDESAIRGYVVNARDITRRK
                     LAEEEVLQSERSLAAELDAARHLQQVSTQLIQADRVEALYEQILDTAVTILAADFASI
                     QMLDVNRGPTGELQLLGHRGFTEEAARFWEWVCPGSESACGKALSTRQRVIIPDIRDC
                     EFLAGSADRDILMQTGIRAVQTTPLYSRSGTLLGMLSTHWRRPHEPTASELRAIDLLA
                     RQAADLIDRRRAEDALQCRTEDLIRKSEELEAARDTANIYLDILTHDVRNANNVSGMY
                     ADLLVGMTEGDLKTYAEKLHASIGRSTEILQNVATIRRAQEEAGLVPVNLDAVIREEI
                     ATFRGATIRYAGHEVNVRADGLLPTVFTNLIGNAIKHGGPGVVVTVRVEERDGEVLVS
                     VEDTGQGVPDAVKKRLFTRFERGMARGSGQGLGLFIVRTLVARYGGRVSIEDRVPGHP
                     EEGAAFRFTLKRVAQDG"
BASE COUNT          686 a         1078 c         1128 g          583 t
ORIGIN      
        1 tgtaattatt ttgttatggt acgatcatcc atcgcggtaa accggattgg ggccccggcg
       61 ggtgagcggt ccgcaacgag ggggccccgg ggtgagagag aagcaaactc ttttctaccc
      121 ccgtgaacaa aggatcactg atgcccgacc gatcccggga ccagcgtggt cccgggcaga
      181 tcgaaccttt actctcaccg cttcatcccg taaataattt tatgcgttaa tatttataga
      241 aattgagttt gaacttgcag gagtaccgac aacgtgagtc agccacaccc cccttcatcc
      301 gggtcgctcg caggcccatc cattctcgat gccctcaccg agggcgtact cctgatcgac
      361 cgggagcagc gggtggcttg gatcaaccgc ccgctcgagc gccttctcgg catcgatcgg
      421 gaccccatcc acggcagcaa cgcaggagaa ttcgtgcgcc ggcacctcgg gccgtgcatc
      481 atcgacaggg cgtgtgcgga tcgggtagcg gcatcactct cgatgtcgca gagcagcatc
      541 gaggtcacct gcacgttccg gtcacccggc ggccgggtca tcatatcgga tgtcatcctg
      601 caggacgaac cgttccgggg catgcgcctc gtccggttcc agggtatcgc cgggcagaaa
      661 caggcggagg atgccctccg ggagagcgat gagacgtatc ggcagatctt cgcgtcgatg
      721 ggggtgggtt tcgcgttcta cgagatggtc cgcgacagcg ccgggcgacc cgtcgactac
      781 ctcatcctcg acatcgatcc tgcctatgag catatcatgg gggcgcaccg cgaccggatc
      841 gtgggccaaa ggttcaccga gatctatccc ggtatcgaac cgctctggat agaacgctac
      901 gataaggttc ttcgcacgca aaaaccggcg cggttcgagg agtacaaccc gaaatccggc
      961 cggtggtacg acatcgacgc ctacccgatg cgggaagacg accggttcgc ggtcatcttc
     1021 accgagatca ccggatggaa gcaggcagaa gaggcgttgc aggagagcga agagaggtat
     1081 cgtaccctct ttgagagtgc aagcgacgcg ctcttcatcc acgatcgcaa cgggaagatc
     1141 ctcgacgtaa accgggcagc ggtcgagcgc cttggctact cacgggaaga actgctggca
     1201 atatgcctca ccgacatcgt cctgccggag tttgccgctc tcgtgcccga ccagacccgg
     1261 gcggtcgtgg agcgcggcag gatgatcgtt gagaccgccc atctcgcccg cgacgggagg
     1321 cgcatccccg tcgagacctc atcacaggtc atcccgtacc ggaaggggac ggctatcctt
     1381 gccgtctccc gggacatcac ccggcgaaag cgctcggaag aggttctgcg ccatcaggca
     1441 gagatgtttg agcggctcat cgatacgata ccggtcatga tcgtcatatt cgacccggaa
     1501 ctgcggcagt tccggttcaa ccgggcgttc cgcgagaccc tgggctggac cgaggaggat
     1561 attgccggcc gtgatctcat gaccgtggtc tatcccgacc cggactaccg ggcaaaagcc
     1621 gcggcgttca tgcagtcgct tgaaccgggc tggcgggagt ggaaggtgac ggcaaaagac
     1681 ggcagcatcg tcgagagttc ctgggcaaac atcagccttg cggacacgac ccggatcggg
     1741 atcggcatcg acctccggga gcggaaacgt gcggaggaag ccctgcgggc gagcgagaag
     1801 cggttccggc tgctctccga gaacgcttca gacatcgtcc tcgtctttga tgagcaaggg
     1861 atcatatcgt atgcgagccc ctcggtgaga ccgatcggcg ggtatgcgcc cgaagacctc
     1921 atcgggaggt gcatcttcga gtttgtgcat cccgacgacc tgccgccggc ccgggatgcc
     1981 ctgcagcgtg ccgccgccca cccgggcagg caggtcaccc tcgaagtcag ggttcggcac
     2041 agcggggagg actggctgta tctcgacctg accgtcaaca acctcctgga tgagtcggcc
     2101 atccggggct acgtagtgaa cgcccgcgac atcaccaggc ggaagttggc ggaagaagag
     2161 gtactgcaga gcgaacggtc cctcgcggcg gagctggacg ctgcacgcca cctccagcag
     2221 gtcagtacgc agttgatcca ggccgacagg gtcgaggcgc tctacgagca gatcctcgat
     2281 accgcggtga cgatcctcgc cgccgacttc gcgagcatcc agatgctcga tgtaaaccgc
     2341 gggcccacgg gagaactcca gctcctcggc caccgcgggt tcaccgaaga ggccgcacgg
     2401 ttctgggagt gggtgtgccc cggttccgag agcgcctgcg gaaaagcgct ctccaccagg
     2461 cagcgcgtta ttataccgga tatccgggac tgcgagttcc tggccggcag cgccgaccgg
     2521 gacatactca tgcagaccgg catccgcgcc gtgcagacca cgccgctcta ctcgcgttcc
     2581 ggcaccctgc tcggcatgct ctccacgcac tggcgccggc cgcacgagcc cacggcaagc
     2641 gaactgcgtg caatcgacct cctggcgcgc caggcggcgg atctcatcga ccgcaggcgg
     2701 gcagaggatg ctctccagtg ccgcaccgaa gacctcatcc ggaagagcga agaactcgaa
     2761 gctgcccgcg ataccgccaa catctacctc gacatactga cccacgacgt ccggaacgca
     2821 aacaacgtct cgggcatgta cgccgacctc ctggttggta tgacggaagg cgacctgaag
     2881 acctacgcag agaagttgca tgccagcatc ggccggagca ccgagatcct ccagaacgtc
     2941 gccaccatcc ggcgggccca ggaggaggcg gggcttgtgc cggtgaacct cgatgcggtc
     3001 atcagggagg agatcgccac ttttcgtggc gcaaccatcc ggtatgccgg tcatgaggtc
     3061 aacgtgcggg cggatggcct cctgccgacc gtctttacaa acctcatcgg gaacgccatc
     3121 aaacatggcg gccccggtgt cgtcgtcacc gtccgggtcg aggagcggga cggcgaggtg
     3181 ctggtctcgg tcgaggatac cggccagggg gtgccggatg cggtgaagaa gaggcttttc
     3241 acgcggtttg aacggggcat ggcccggggg agcggtcagg gtctcggcct cttcatcgtc
     3301 cggacgcttg tcgcgcggta cgggggccgg gtctctatcg aagacagggt gccgggccat
     3361 ccagaggaag gggcggcctt ccggtttaca ctgaagagag tcgcgcagga cgggtgaacc
     3421 cgggcgcacg gttcgggggc gggccgatgc agccggcggg gaggcacgga agggg
//
LOCUS       DAOSEE010000188         3459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_9618_length_3459_cov_233.313749, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000188 DAOSEE010000000
VERSION     DAOSEE010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9618_length_3459_cov_233.313749"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..1130)
                     /locus_tag="PLI76_10555"
     CDS             complement(144..1130)
                     /locus_tag="PLI76_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosine biosynthesis radical SAM protein
                     RaSEA"
                     /protein_id="HOI62516.1"
                     /translation="MVSKSIEKPLASWRGKDLYEGQVLDTLTVIFRSGGCSWNRCRMC
                     GYRHERYPDLPQDDLADRLIRQVRWVKENFCDEDYQVLKIFTSGSFFDPDEVPPAVRR
                     AVAEAFRGKAVIAETRPEYVESDALREFLEGIDTGVWSRPLSVAVGLETTNDAIREKS
                     IDKGFTYADFLHAAGIAHAAGAGVKAYLLMKPPFLTEREACDDMVRSIRDVASVADSI
                     SMNLCTVQSRTEVERLWKEHAYRPPYLWSVLDVLIKSPVHILCDPVGGGQTRGPHNCG
                     ACDGPIVKGIGDYSLTGDVELLRALAETDCGCKEEWMFVLDREEPFCMPLTR"
     gene            1159..2247
                     /locus_tag="PLI76_10560"
     CDS             1159..2247
                     /locus_tag="PLI76_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62517.1"
                     /translation="MKRIAWLTLIAAMLLVSAASAAYVSISAPKEVYVGDRLVVAGTS
                     VAGEFAQPSLKPGFSTDVILYYAKGTKSEVARKTIVVQENGLFSATFDTAGLAAGTYS
                     IEIVDPTQTTFGGSSKVQQMVELIDRSKDIVVDSPLTQEFDGTLDLRGTVSGIGDAGV
                     QVRVEHDAAVIYGPKYIRTDANGAFSEEVPIPDGGTYGVTFSDTKGFISTVTFVISGK
                     PAPTSTPAMISANAPATRSAPAYFEVDTKSGTVTITTSVGIDWVIEYIDEDGGSHKVN
                     DKGQLEPETAEFVARGGTVYVKVYPMSYSDRGTVQVSAANADSIRVSQAASGLFGDAV
                     PTTAQAAPLPAFLALLALIVVVVLARRG"
     gene            2287..2371
                     /locus_tag="PLI76_10565"
     tRNA            2287..2371
                     /locus_tag="PLI76_10565"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2321..2323,aa:Ser,seq:tga)
     gene            complement(2541..>3459)
                     /locus_tag="PLI76_10570"
     CDS             complement(2541..>3459)
                     /locus_tag="PLI76_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOI62518.1"
                     /translation="SVEKKVEAVCQHSPDIVAFQEVTDRDLPLFREGLENFGLTHFVD
                     SLALVEIARVAYMAERLNDLRARNTNDPFQFAYSISRLSEQCSPVGETKDAGCLIASR
                     YPLAPLNPLDFDIPWKQRVVSAVVSAPTGEFEVHNVHVPTAIGSRRNEIVKAETLVGI
                     YRHLAVRPARPRILCGDLHIPEAELPDGTIVPFYRDIIPDRTYTTASESDEYLGRPRR
                     WWYNAEMNVISGLAEYDLPDVFRKLHGYRAREYSWCPDRGPEDVPKCKRYDHIFASAR
                     LNPTACWYLHDLRGQGLSDHSAVVMDFEP"
BASE COUNT          647 a         1077 c         1075 g          660 t
ORIGIN      
        1 tgggtatccc tgcgaaggcg cgggtggggt ggaggaggcc ggcgtatagg tatcccctgt
       61 gcgggcgact gcggtaacca ttacgctcct cggcccgggt tctcccccgg gctccggaac
      121 cggtatcgcc ggaacccggc ggactaccgg gtgagcggca tacagaacgg ttcttcccgg
      181 tcgagcacga acatccactc ctccttgcac ccgcagtccg tctccgcgag ggcgcgcagg
      241 agctcgacgt cgccggtcag cgagtagtcg ccgatcccct tcacgatcgg gccgtcgcag
      301 gcgccgcagt tatgcggccc ccgggtctgc ccgccgccga cggggtcgca gaggatatgg
      361 accggggatt tgatcagcac atcgaggacg ctccagaggt agggagggcg gtatgcgtgc
      421 tctttccaga ggcgttcgac ctcggtcctg ctctggacgg tgcagaggtt catggagatg
      481 ctgtccgcaa cggatgcgac atccctgatg gagcgcacca tgtcgtcgca cgcctcgcgc
      541 tcggtcaaaa acggcggttt catcaggagg taagccttca cgccggcgcc ggcggcgtgc
      601 gcgatccctg cggcgtggag gaaatcggcg taggtgaacc ctttatcgat gctcttctcc
      661 cggatagcat cgttcgtggt ctcaagcccc acggcgacgg agagcggccg cgaccagacg
      721 ccggtatcga taccctcgag aaactcacgg agcgcgtccg attcaacgta ttcgggcctt
      781 gtctcggcga tgacggcctt accacggaac gcttccgcaa cggcgcgccg gacggcaggg
      841 ggaacctcgt cgggatcgaa gaaactgccc gacgtgaata tcttgagcac ctggtagtcc
      901 tcgtcgcaga agttctcttt cacccagcga acctggcgga tcaaccggtc ggcaaggtcg
      961 tcctgcggca gatcagggta gcgctcgtgc cggtagccgc acatacggca ccggttccag
     1021 gaacacccgc cgctcctgaa gataaccgtc agcgtatcga ggacctgccc ttcgtagagg
     1081 tctttccccc gccaggacgc gagcggcttc tcaatcgatt tggataccat tatacctcat
     1141 attcgttgat gtactggtat gaaaaggatc gcatggctga cgctcatcgc tgcgatgctg
     1201 ctcgtcagcg cggcatcagc cgcgtatgtt tcaatcagtg ccccaaaaga ggtatacgtc
     1261 ggtgaccggc tcgttgtcgc cggcacatcg gttgccggag aattcgcaca gccttcgctc
     1321 aaaccggggt tcagcacgga cgtcatcctg tactacgcca agggcacgaa gagcgaggta
     1381 gcccgaaaga ctatcgtcgt tcaggagaat ggtttgttct cagcgacgtt cgatactgcc
     1441 ggtcttgctg cgggaaccta ctcgatcgag atcgtcgatc ccacgcagac cacattcggg
     1501 ggcagttcga aggtccagca gatggtcgag cttatcgacc gctccaagga tatcgtggta
     1561 gattccccgc tcacccagga gttcgacggc acgctcgacc tccggggcac ggtcagcggg
     1621 atcggcgatg caggcgttca ggtccgggtc gagcatgatg ccgccgtcat atacgggccg
     1681 aagtacattc gcaccgatgc aaacggagca ttctccgaag aagtgcccat ccctgacggc
     1741 ggcacctacg gggtcacatt ctccgacacc aaaggtttca tcagcaccgt gacgttcgtg
     1801 atctcgggta agcccgctcc cacgtcgacg ccggcgatga tatccgcgaa cgcccccgcc
     1861 acgcgctccg ccccggccta cttcgaggtc gacacgaaga gcggtacggt gacgatcacg
     1921 acatcggtcg ggatcgactg ggtgatcgag tacatcgatg aggatggggg atctcacaaa
     1981 gtcaacgaca aaggccagct cgaacctgag accgcggagt ttgtcgcacg gggcggcacc
     2041 gtctacgtca aggtctaccc catgagttac agcgatcgtg gaacggtgca ggtctcggcc
     2101 gcgaacgccg actcgatcag ggtgagccag gctgcgtccg gactcttcgg ggatgcggtg
     2161 cccaccaccg cccaggccgc gccgctcccg gcgttcctcg cgctccttgc actcattgtt
     2221 gtggtcgtcc tcgcgcgccg gggatgaaca accttttttc gtccggcggc caataggtat
     2281 aggcgggccg ggatagtcta gtccgggaag gcggcggcct tgaaagccgc tggtgcctgg
     2341 cacctcggga gttcaaatct ccctcccggc gtcctttgtt ctgttcatat ccgtaacggt
     2401 tgttgcgccc cattcgtaca tccgatcgca ctcaccggcg ccagaaccct ggagaaacta
     2461 taccagcaag cgatgccccg gggcgataga gtatcttcgt gccttatagg gagagggcgg
     2521 gatcgtgacc gggagtgcgg tcacggctca aaatccatca cgaccgcgga gtggtcgctc
     2581 aacccctgcc cccggaggtc gtgcaggtac cagcacgccg tcgggttgag gcgcgcagat
     2641 gcaaatatgt ggtcgtagcg cttgcacttc gggacatcct cgggcccgcg gtcggggcac
     2701 cagctgtact ccctcgcccg gtagccgtgc agtttccgga agacatcggg gaggtcgtac
     2761 tccgcaaggc cggatatgac gttcatctcg gcgttatacc accaccttct cggccgcccg
     2821 aggtactcat cgctctcgga ggccgtcgtg taggtccgat cagggatgat atcgcggtag
     2881 aaggggacga tcgtcccgtc gggaagttcc gcctccggta tgtgaaggtc gccgcagagg
     2941 atccgtggcc tcgctggcct gacggcaaga tgtcggtaga tcccgaccag tgtctcggcc
     3001 ttcacgatct cgttcctccg gctgcctatc gccgtgggaa cgtggacgtt atgcacctcg
     3061 aactcgcccg tgggtgcact gacgacggcc gagaccaccc gctgtttcca cgggatatcg
     3121 aagtcgagcg gatttaatgg cgcaaggggg tacctgctcg cgatcaggca tccggcgtcc
     3181 tttgtctcgc cgaccggcga gcactgttcg gagagacgcg agatgctgta cgcgaactgg
     3241 aagggatcgt ttgtgttgcg cgcacggaga tcgttcaacc gctcggccat gtaggccacc
     3301 cgggcaatct ccaccagagc aaggctgtcg acgaaatgag ttaacccgaa gttctccaat
     3361 ccctcacgaa agagcggcag atcccgatcc gtcacctcct ggaaggcgac gatatccggc
     3421 gagtgctggc agactgcttc gactttcttc tccacgctc
//
LOCUS       DAOSEE010000189         3414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_9782_length_3414_cov_247.358142, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000189 DAOSEE010000000
VERSION     DAOSEE010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9782_length_3414_cov_247.358142"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..372
                     /locus_tag="PLI76_10575"
     CDS             58..372
                     /locus_tag="PLI76_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62519.1"
                     /translation="MLTASLVRWQNTGSGSGAPASGGSGSSGRLPRGKESVILIELEE
                     GGRSFDTYMDLKFSLEDLFGRKADLIDRDAVNHAYFGTHNEVVGDAVRNRVLELQVAD
                     AR"
     gene            complement(508..2190)
                     /locus_tag="PLI76_10580"
     CDS             complement(508..2190)
                     /locus_tag="PLI76_10580"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HOI62520.1"
                     /translation="MPASRRSHSLTRIFQQAAGKKHIVASQTPLIAIVAASFLDRIEF
                     VETIDAALDRDPDRWRVSPGNLAKGIVLLPFIHSGPRLPILNVSECYQGMDMELLFTS
                     EVRPEWLTRDALACMLDGLSAAGCEHLFTSLALRVYTAFSIPLYPVLHGEATPISLYG
                     VYEEVEGSPLSAPAASREERRRGPVTVTLGQACDPRGIPLITTVHGGDEADSAWSADV
                     IRTLAELNPFPRTDVTAIADPKLATAQNIRTLQKKGFRFVTRCPASFGKKVAARVTRA
                     AYEADAWIPVGSCRDTGEDTLEACDVQEFAKATDDGVSCRFLVFRDNCQRHKFEADLA
                     KKREEFVAVLKEVAGREFVREPDARQALEEARKRLRRYSPWEADLAIETAVTGKNSRA
                     AGWAIRAGDPQLQQEVYLAELHGAESFVLMTNVPGEDLPAREVLCLYTGQKVVRDNFS
                     VIRRPAMVDTLYLEKPGRIAALGTLLSATLLIQMVIRVLVRRNLDALPEPPGLDHGCK
                     PLIRPGLKKILRFIGYYSIITTGGERRFWCISSSHEKNLEIWLRLLELDKGR"
     gene            2751..>3414
                     /locus_tag="PLI76_10585"
     CDS             2751..>3414
                     /locus_tag="PLI76_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOI62521.1"
                     /translation="MMAKKDAGNRQTSLNSCSGGEAVSRAKGTPADRSASALPAIASA
                     RDLDRHLRTFRHQRDGVITDTVRPARVEEVNVGDTVVRRYIGEFWTAKQRGASTLHEV
                     SYRACFKPQLPRFFIDRLTAEGDSVYDPFGGRGTTIIEATLAGRRGISNDVNPLSEIF
                     ASPRLHIPGLSDIEKRLREIPLDKTKTADIDLSMFYHAETLGEIVSLKEYLQERRATG
                     KED"
BASE COUNT          692 a         1021 c         1095 g          606 t
ORIGIN      
        1 ccgcggcacg ataccggggg aagagcgtta tcctgatgag cggataacag atacggcatg
       61 cttacggcat ccctggtgcg ctggcagaac accgggagcg gatcaggagc cccggcgtcc
      121 ggcggatcgg gatcttcggg tcgtttgccc cggggcaaag agagcgttat cctcatcgag
      181 ctcgaagaag ggggccgttc ctttgacacg tatatggacc tcaagttctc cctcgaagac
      241 ctcttcggga gaaaggccga cctcatcgat cgcgacgccg tgaaccacgc gtacttcggg
      301 acccataacg aggtcgtggg ggatgccgtc cggaacaggg tccttgaact ccaggttgca
      361 gatgctcgat agcgaggcaa cttgaaattc gcaaccgtgt catcgcccgt gcggttcgag
      421 aaccgggtct gatcgagcaa tgggggtgca ggctcgtgac ggccggcgct gacggcgccg
      481 caacggtcga ggagaagagc gcagatgtca cctacctttg tcgagctcca gcaggcgcaa
      541 ccagatctcc aggttcttct catggctcga cgagatgcac cagaaccggc gttcgccgcc
      601 ggtggtgatg atcgagtagt agccgatgaa ccggaggatc ttcttcaagc ccggccgaat
      661 gagcggcttg cagccatgat cgagccccgg cggctctggc agagcatcca ggtttcggcg
      721 caccagcacc cggatcacca tctggatcag gagagtcgcc gacagcagtg tcccgagcgc
      781 cgcaatccgc ccgggtttct ccaggtaaag ggtatcgacc atcgccggcc gcctgatgac
      841 cgagaagttg tccctgacca ccttctgccc cgtatacagg cagagaacct cacgtgccgg
      901 cagatcctcc cccggaacgt ttgtcatcag gacaaacgac tcggccccgt gcagctcggc
      961 caggtagacc tcctgctgca actgcgggtc gccggcccgg atcgcccacc cggcggcccg
     1021 tgaattcttc ccggtgaccg ccgtctcgat ggccagatcc gcttcccagg gcgaatacct
     1081 gcggagcctc ttacgtgcct cttcgagggc ctggcgtgcg tcaggctcgc ggacaaactc
     1141 tctcccggcc acctccttga gaactgcaac gaactcttcc cgcttctttg ccagatcggc
     1201 ctcgaattta tgtctctggc agttgtcccg gaaaacgaga aaacggcagg ataccccgtc
     1261 atctgttgct tttgcgaact cctgtacgtc gcaggcctca agcgtatcct caccggtatc
     1321 ccggcatgac ccgacaggta tccaggcatc cgcctcgtag gccgcccggg tcacccgggc
     1381 agccaccttc ttgccgaaac ttgcgggaca ccgggtgacg aaccggaagc ccttcttctg
     1441 gagcgttcgg atgttctggg cggttgccag tttcggatcg gcgatggcgg tgacatcggt
     1501 acgggggaac gggttcagct cggcaagggt ccggataacg tctgcgctcc aggcagaatc
     1561 tgcctcgtcc ccgccatgaa cggtggtgat gagcgggata ccgcgggggt cgcaggcctg
     1621 gccgagcgtg accgtgacgg gaccccttcg ccgctcttcg cggctggcgg cgggagccga
     1681 aaggggagag ccttcaacct cctcgtagac gccgtacagg gagatcgggg tggcttcgcc
     1741 atggaggacc gggtacaacg gaatactgaa cgcggtgtag acccggaggg caagggaggt
     1801 gaagaggtgc tcacacccgg cggcagagag cccgtccagc atgcacgcga gtgcatcccg
     1861 tgtcagccac tcggggcgca cctcggaggt gaagaggagc tccatatcca tcccctggta
     1921 gcattcggag acattgagga tggggaggcg cgggccggaa tggatgaacg gaagcaggac
     1981 gatacctttc gccagattac cggggctcac ccgccaccgg tccggatccc ggtcgagtgc
     2041 cgcatcgatg gtctcgacga actcgatccg gtcgaggaac gaagcggcga cgatggcgat
     2101 gagaggcgtc tgggaggcaa caatgtgctt cttcccggca gcctgctgga atatgcgggt
     2161 cagagaatga ctacgtctgg aagcgggcac aggaagtgta aagtggggta atgcttatgt
     2221 ttttcgatcc gtgaggaacc gcatgatgct tcagcagggg ggttgcgaag cgagcgatcc
     2281 cggatgaggg cgataggatc gaggaggctc cggggcgacc tccggtcgct ctccggcccg
     2341 aatgccggcc ggttatcgca aggatcccgg gccgcttctc ccggatcgga tgcggcgtga
     2401 cggttcaata cgccgcatcg gaagagcgga agcccccctc ccgggtcgtg gcaggccggc
     2461 tccgatgccc ccgcagctag agagagcccg gcaaacggat ccccgtcagg cgggtgtggc
     2521 gaccaaacgg aggatgcagc ggcgctcatc accggcgatg ggcaccgggc tgccacgatc
     2581 gcagagatgt tcgcgggatt agtgacggcg aagagtcgct gagagatggg cggtgatgct
     2641 cttttgagtc cgcgctatgc cgcaggatcg gtacgagggc gactctatac cgtgcagccc
     2701 tgagaatgct tgatagcccg tgaataccat acgtttaccc gggaatcatc atgatggcga
     2761 agaaggatgc ggggaacagg cagacttccc tcaactcttg ctccggggga gaggcggtat
     2821 ccagagcgaa aggaacgccg gcggacagat cagcctctgc cctccctgca atcgccagcg
     2881 ccagggatct cgatcgccat ctccgcacct tccgtcatca acgggatggg gtgataaccg
     2941 ataccgtacg acctgcccgt gtcgaagagg tgaatgtcgg ggatacggtc gttcgaaggt
     3001 atatcggtga gttctggacc gccaagcaga gaggtgcaag cactctgcac gaggtctcct
     3061 accgcgcctg cttcaaaccg caactccccc gattcttcat cgaccgctta actgcagagg
     3121 gggatagcgt ctacgacccc tttggcgggc gcgggacaac catcatcgag gcgacgttag
     3181 caggccggag gggaatatca aacgatgtca atcccctgag cgagatcttc gcctcccccc
     3241 gcctgcatat cccggggtta tccgatatcg agaagaggct ccgggagata cccctggata
     3301 aaacaaaaac agcggatatc gatctctcca tgttttacca tgcggagacg ctcggggaga
     3361 tcgtatccct taaagagtac ctccaggaac gccgggcaac cggcaaggag gatg
//
LOCUS       DAOSEE010000190         3405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_9818_length_3405_cov_266.814030, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000190 DAOSEE010000000
VERSION     DAOSEE010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9818_length_3405_cov_266.814030"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(248..1624)
                     /locus_tag="PLI76_10590"
     CDS             complement(248..1624)
                     /locus_tag="PLI76_10590"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HOI62522.1"
                     /translation="MILKPYVVSLTLATLFILLLTTGCASQQDGPGQAGIIPQTMSPS
                     AGVDSMTLVATDGNASLYKIGNISVIRVEGSYREMGRQYGRLVGTDIAKMYETIVASI
                     GEPGFIIGLESEEQLVNFSMRQYSLYPQEYREIVEGMAESSGIPVAHIAVIDQVVPTF
                     ALYPQTELTPDRVQAILAGKVDPTPGNTSAIPDGHHCSSIIAWGDYTGGGPLVMGRNF
                     DFGQAYRHFNPYRSVIVYNPTDGSVPTAVLGWPGTVGGIEMFNRAGLVMETDDNHVVT
                     APNNEVHIDRIPLTVLVLRLGMDSATIEQFDAAMKTARFGYPLLSNVATEEEGYTYEI
                     GTRDVVRRGDDRYGLQVIANFPLAPYWDEPSDMTDLDTGLSISRRNNLIALAEKYKGA
                     INATVMEAILDTRYADGGASFDTAPEGGSVTIYQFVYIPKTRMLYLKAPTYDDWTAVP
                     LAPLFKNP"
     gene            complement(1926..2378)
                     /locus_tag="PLI76_10595"
     CDS             complement(1926..2378)
                     /locus_tag="PLI76_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOI62523.1"
                     /translation="MTTLYLARWETRFWAWLIDIILVGLPLWALSDRLPPAWRFTIDP
                     GLMSVSLSSVVLFLYWMLLEGYRGQSIGKMAMNIRVTGRAGEGVGFAAAAIESFGKAF
                     LLIPDCLIGWLAMPGSKQRLFNRISHTIVIENRNAEEPEGVTYVKQEE"
     gene            complement(2483..2662)
                     /locus_tag="PLI76_10600"
     CDS             complement(2483..2662)
                     /locus_tag="PLI76_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62524.1"
                     /translation="MSRSSSTASVPLARAGAGLPKVDLPIALPHALRPGRCRMTGSRC
                     GAGKNPPGDGLARQG"
     gene            2914..>3405
                     /locus_tag="PLI76_10605"
     CDS             2914..>3405
                     /locus_tag="PLI76_10605"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOI62525.1"
                     /translation="MAKANIPAKGAIVQRDFETFSVAPHIPGGFIEPALLRKIADVAE
                     KYGAKFVKLTGTQRMIIVGIQEEDLDKVWAEFEDSSKAIGLTIRSIQMCPGTRSCKRA
                     KQDSPGLGFALDKEFYGKPVPAKFKMAVSGCSNCCSDPWMRDLGFIGTDDGFKAVVGG
                     KGGG"
BASE COUNT          712 a          971 c         1054 g          668 t
ORIGIN      
        1 atcttacacc gacgttgaca ggggacatct cctccggcat aggggtcatg ggataacctc
       61 atactcaccg gtgccgccgt tctgacatta caggtatcgg ggggagaggc acagccgagg
      121 tgtctcaaac gggcaaagtc tcggagttga aagagcattg agaagggggt gccgtctttt
      181 attctcccct gcccgaagac cggggacttc ccgcttcgcc ccttcacccc cgcaagttaa
      241 aaaaaggtca gggattttta aacagcggtg cgagagggac tgccgtccag tcatcgtagg
      301 tgggggcctt gagatagagc atccgggtct tagggatgta gacgaactgg taaatagtga
      361 cgctgccccc ttcgggagcg gtatcgaagg aggccccgcc atctgcgtaa cgggtgtcga
      421 ggatcgcctc catgaccgtt gcgttgatgg cgcccttgta cttctcagcc agggcgatga
      481 ggttgtttcg ccgcgatatc gagagccctg tgtccagatc ggtcatgtcg gacggttcgt
      541 cccagtatgg agcgaggggg aaattggcga tgacctgaag gccgtacctg tcgtcgcctc
      601 gccgcacgac gtcacgggtc ccgatctcgt aggtgtaacc ctcttcctcg gttgccacgt
      661 tcgagagcag ggggtaccca aaccgcgccg tcttcatggc cgcatcgaac tgctcgatcg
      721 tcgccgaatc catcccgagc ctgaggacga ggacggtcaa cggaatccgg tcgatatgaa
      781 cctcgttgtt gggggcggtg acgacgtggt tgtcgtcggt ctccataacg aggccggccc
      841 ggttgaacat ctcgattccc ccgacggttc ccggccaccc aagaactgcc gtcggaacgc
      901 tcccgtcggt cgggttgtac acaatcacgc tccggtaggg attgaagtga cggtacgcct
      961 ggccgaagtc gaagttcctg cccatgacga gcggaccgcc gccggtgtaa tcgccccagg
     1021 cgatgatcga agagcaatgg tgcccgtcag gtatagcgga cgtgttgccg ggggtgggat
     1081 cgaccttgcc ggcgaggatc gcctgtaccc gatccggtgt gagctcggtc tgggggtaga
     1141 gggcgaaagt gggtacaacc tggtcgatga cggcgatatg cgcgaccgga attccgctcg
     1201 attcagccat gccttctacg atctcccggt actcctgagg gtagagggag tactggcgca
     1261 tgctgaaatt gaccagttgc tcttcggatt cgagaccgat gatgaaaccc ggctcgccga
     1321 tggatgcgac tatcgtttcg tacatcttcg ctatgtctgt acccacgagt cgcccgtatt
     1381 gccggcccat ctcgcggtac gaaccttcga cccggatgac ggagatattc ccgatcttat
     1441 agagacttgc gtttccgtcc gtggcaacca gcgtcatgct gtcgacgcct gcggatggac
     1501 tcatcgtctg ggggatgata ccggcctggc cgggaccgtc ctgctgagag gcacagcctg
     1561 tagtgagcag gaggatgaac agggttgcca gggtcaacga aacaacataa ggtttcagga
     1621 tcaaccactc ctcggttaac cagctgttcg ccataaaaag atttagttat tagcattcat
     1681 cgttcactcg ccgcccacgg ataaactcat tttgtgagac cgctcaggca aagaagtgca
     1741 aaaagcggaa gagaacaaaa gaggtccggc agggagtcac cgctccagtc agcactgccc
     1801 tgaccttacg ttcgccggca cgccggcaaa cggatcaacg aagagaaccc cacagggaac
     1861 ggaatatagg gggcagattc ttgctacact gtcgcaagaa ttggtgcacg atgaatgatc
     1921 gatcctcact cctcctgctt cacgtaggtg acgccttccg gctcctcagc attacggttc
     1981 tctatcacga tggtgtggga gatccggttg aagagcctct gctttgagcc cggcatggca
     2041 agccacccta tcaggcagtc ggggatgagg aggaacgcct tcccgaaact ctcgattgcc
     2101 gcggcggcaa aaccgacccc ttccccggca cggccggtga cccggatgtt catggccatc
     2161 tttcctatgg actggccccg gtacccctca agcagcatcc agtacaggaa gaggacgacc
     2221 gaggagaggc ttaccgacat aagacccgga tcgatcgtga acctccaggc aggcggcagc
     2281 cggtcggaga gcgcccagag gggcagcccg accaggatga tgtcgatgag ccatgcccag
     2341 aagcgggtct cccaccgggc gaggtagagg gtggtcatac gtatctacct gtcgtttcct
     2401 gtaactcata acggtttgta tggctggcct gcaggcgccc ctggagaaga ggctgctatg
     2461 cggcggtacc gggcagtttc tctcatccct gccgggcaag gccgtccccc ggggggttct
     2521 tgccggcacc gcagcgactc ccggtcatcc ggcacctgcc cgggcgaagg gcatggggga
     2581 gagcgatcgg tagatcgacc ttcggcaggc cggcacctgc ccgggcgagg ggcacggatg
     2641 ccgtcgatga agatctcgac atacggttac ccggcatcag ccccggtgag ggagatgccg
     2701 ggaccgggtc ttctccgcgg cctctctccc tgcaatgatt gcggtcgacc gggtagaacc
     2761 gtaccaatcc cgccgcggga tggggtaagg ggagccacac cgacaagaaa gactcctttt
     2821 ttgcttttga gggcttccag gatgccggat ctatttcgca agatattaac gggagtgatg
     2881 acaaccctgg atcctatcta attggttgat atcatggcaa aggcaaatat tcctgcaaaa
     2941 ggggcgattg tccagagaga tttcgagacg ttctcggtcg cgcctcacat ccccggcggt
     3001 ttcatcgagc cggcactcct gaggaagatc gcggatgtgg cggagaagta cggtgccaag
     3061 ttcgtgaaac tcacgggcac ccagaggatg atcatcgtcg gcatccagga ggaggatctc
     3121 gataaggtat gggccgagtt cgaggacagc tccaaggcaa tcggcctgac catacggagc
     3181 atccagatgt gccccggcac ccggtcctgt aaacgtgcca aacaggacag ccccggtctg
     3241 ggatttgccc tcgacaaaga gttctacggc aaaccggtgc ccgcgaagtt caagatggcc
     3301 gtctcgggtt gctccaactg ctgcagcgac ccctggatga gggacctcgg gttcatcggc
     3361 accgacgacg gattcaaggc ggtcgtcggc ggcaagggcg gcgga
//
LOCUS       DAOSEE010000191         3355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_10009_length_3355_cov_260.613333, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000191 DAOSEE010000000
VERSION     DAOSEE010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10009_length_3355_cov_260.613333"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="PLI76_10610"
     CDS             <1..1131
                     /locus_tag="PLI76_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62526.1"
                     /translation="EDYTINATCAAIGEHCYVFVEEGLPFARMGVADLMDAFDHVIHP
                     VVTTTFGSGIGIDRDPRVYIVLLDIRDDSRYNSTSASIAGYFDGSTKNNIDLVFLDID
                     RRGAEMRSTLAHEYQHLIHHSLDAHERKWIDEGCSGYAELLCFDNQNQAKVRAYNRLP
                     ETPLVVTDNQWDSSDDEMTRAHYGASFLWILYLAENYGDRSGHPDHGSFLKDLVANEL
                     PGMQGVTTTLAQHGFEESFEDVFKRWTIANYLDMDGADPPFGYASINITRHPQRAGWV
                     DLPAQNELSYSFPRKELPPWSAAYYEVEARDPDLVSYSNDRGFWMEKVVNRAGEAVIV
                     VSPLEGRGAFVLTVSEVTGNRSAGTAASGLPSLTPPATLVAG"
     gene            1273..1343
                     /locus_tag="PLI76_10615"
     tRNA            1273..1343
                     /locus_tag="PLI76_10615"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1305..1307,aa:Gly,seq:tcc)
     gene            1346..1419
                     /locus_tag="PLI76_10620"
     tRNA            1346..1419
                     /locus_tag="PLI76_10620"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1380..1382,aa:Val,seq:cac)
     gene            complement(1450..1992)
                     /locus_tag="PLI76_10625"
     CDS             complement(1450..1992)
                     /locus_tag="PLI76_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62527.1"
                     /translation="MDFFDEISSKSEYFKHLKRTVTIGIYGSFKGDNITILENFRDFL
                     ASEGYRARMSLDLGYPGVALSEVSQHQKNCLASTKLRESSRIHIIFFFIEGSQEHNVN
                     LSAAMELQALAEHEVRDVLLLFEKGLKKEDITSYFAGTVETHEGRWEWDEFARDTDLH
                     LIRGRQFCLNRMRRYSTGFK"
BASE COUNT          759 a          914 c          967 g          715 t
ORIGIN      
        1 gaggattaca ccatcaacgc aacgtgtgcg gcgatcgggg agcattgtta cgtgttcgtc
       61 gaggaaggtc tgcccttcgc acggatgggg gttgcggatc tcatggatgc cttcgatcat
      121 gtcatacacc ccgtggttac aacgacgttc ggcagcggga taggtatcga cagggatccc
      181 agggtgtaca tcgtgctgct cgatatccgt gacgacagtc gttacaacag cacgagtgcc
      241 tctatagcgg gatatttcga cggcagcacc aaaaacaaca tagacctcgt cttcctggat
      301 atcgataggc ggggggccga gatgagatcg acactcgctc acgaatacca gcacctcatc
      361 catcactccc tcgacgcaca cgagagaaag tggatcgacg aagggtgcag cgggtatgct
      421 gaattgctct gctttgacaa ccagaaccag gcaaaggtcc gggcttacaa ccgccttccc
      481 gaaacccctc tcgtggtgac cgataatcag tgggacagct cggacgacga gatgacccgg
      541 gcgcactacg gggcaagctt cctctggatc ctctacctgg cggagaacta cggggatcga
      601 tcggggcatc ccgatcacgg gtcgttcctg aaggatcttg tggcaaacga gcttcccggg
      661 atgcaggggg tcaccacgac gcttgcacag cacgggtttg aggagtcgtt cgaggacgtc
      721 ttcaaacgct ggacgatcgc caattacctc gatatggacg gggccgatcc gccgtttgga
      781 tatgcgagca tcaacatcac ccggcatcca cagagagccg ggtgggtcga tcttcccgcg
      841 cagaacgagt tatcgtactc gtttcccagg aaggaacttc cgccctggtc tgccgcctac
      901 tacgaggtgg aagcacgcga cccggatctc gtctcgtact cgaacgaccg ggggttctgg
      961 atggagaagg tcgtcaaccg ggccggtgag gcggtcatcg tcgtctcgcc tcttgagggc
     1021 cggggggcat tcgtcctgac ggtatcggag gtgacgggca accggtccgc cggcaccgcg
     1081 gcaagcgggt taccctcgct cacacctcct gcgaccctgg tggccgggtg aaccggcgcc
     1141 ccggcgtgcc ggaggtccgc gggttgaccg ccggctggag tctgccgggg ccaggggacg
     1201 ccgggcggag cccctgcatc cggagatatc ttaataactc atagcgctag aaatgtatag
     1261 acgtaccagt acgcatcgat ggtctagtgg tatgactttg gccttccaag ccaatagccc
     1321 gggttcaatt cccggtcgat gcatggggct cgtggtctag ctggttatga cgtcgccttc
     1381 acacggcgaa gatctcgtgt tcgaatcacg acgagcccat gattattctc cttttgtcga
     1441 atatagatat tatttgaacc cggtagagta gcgtctcatc cggttgaggc aaaactggcg
     1501 accacggatc agatgaagat ccgtatcccg ggcaaactcg tcccattccc accgaccttc
     1561 gtgggtctca acggttcccg cgaaataact ggttatatct tcttttttca aacccttctc
     1621 gaagagaagc agcacgtcgc gaacctcatg ctcggcaagt gcctgtaact ccatagctgc
     1681 tgagagattc acattgtgct cctgtgaccc ttcgataaaa aagaaaatga tatggatgcg
     1741 gcttgattca cgtaatttag tgcttgcaag gcagtttttt tggtgttgag atacttcgga
     1801 gagagcgact cccgggtatc caagatccaa tgacattcgc gcacgatacc cttcggatgc
     1861 taagaagtca cggaagtttt cgagaatagt gatattatcg cctttgaatg agccataaat
     1921 acctatggtt acggttctct tcaggtgctt gaagtattcc gattttgaag agatctcatc
     1981 aaagaaatcc acaaggatac tcattatgtg agaggaaaaa cgttacgtcc ctttagagtc
     2041 acgtttttgt gacagccgtg catctataag tataaataag tcacagatct atgacttata
     2101 ctggtgatga tatggaccgt gcaaagatcg aggaatgtct ggccgaggtc cccacagaga
     2161 tccggagcgc agtgagacct ctcgacaacg ataaagcctg ggcaatctat attctcctgt
     2221 taaaacagcg gcaaatgcga tttaacgaga tcaagaatga atttgacgtt aaatcgtcgg
     2281 gagatgtcga caggtacctg aagggccttg tcaacgccgg cctggtctca aaagaagcga
     2341 ggcgactggg cgaggcggga gacaatgagg cggcctatta cgctcccacg gatttaggca
     2401 gatctctcct gtccgggctg ttccagagtg ttttgccgca ggcgaggccg gcgccgcaca
     2461 atccggccgt cagaaaagaa tcatccggag atggagagtg cgtgagtatt tatccggacg
     2521 ccatcaaaga tcgcagcccg gccctcgtat gccgggattc ctctgccggg tacggagccg
     2581 ggggagttgc cgggcgcagg cagccacgtg tagttacaag gagagaacga tggcgcctga
     2641 aagaaagact gaagcgaaga agagccgggc cgggaagccc gggcggagac cgtcgctcga
     2701 caggtaatat ccgtcgtccg aactccggtt aaacctggca tacgcacagc acgcgaatgg
     2761 attgccggta atttttcgac cggggatgtc gttattgact ttccggagct gcccggacgc
     2821 aaagtggatg ggatacagac gatagggggg tgccgcgtct tctacaccca cacgtatcct
     2881 cccacgtatc cacagccgcg cttcttctaa caatcaatcc cgaaatttcg ggggcaatga
     2941 cggatacccc atcgcgataa cggcctgctg catcgactgg agcccccggg ttaccatctg
     3001 cttcgacgag ccgtgcgcga gcattttgcc ctgcccgtcc gtcacggtcg cctccgatag
     3061 acagacactt catagttgag ggtgctcacg atatccggac tccaatgtag agcgatctct
     3121 ctgtttgatg ataaatcccg ccttctgatg agatctgtcc ggtcgggcgg cgtggggtag
     3181 cccggccggc cccctcacaa cgccttgatc acgctcccga ccacggactc gagatccccg
     3241 cccgtgcatg cgacggtgag atcggcatac cgctcataca acgggatgcg ctcgtcgtac
     3301 atctcgcgga ggctccggcc cggaaggagg agaatcccgc gggtcgtgat gtttt
//
LOCUS       DAOSEE010000192         3336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_10093_length_3336_cov_221.318805, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000192 DAOSEE010000000
VERSION     DAOSEE010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10093_length_3336_cov_221.318805"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..585
                     /locus_tag="PLI76_10630"
     CDS             76..585
                     /locus_tag="PLI76_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HOI62528.1"
                     /translation="MVYMKHRAQILAYSGTFVALIAAGSWISIPLPPVPITLQTLFVL
                     LAGIVMKRYAVIPVALYVLLGAMSLPVFHNGTAGVGVLLGPTGGFLIGFIPAALIAGL
                     AYEQESPKWRIGGLIAACAIYLSAGAVWLSYSASIPLLQAILIGVAPFIVGDTLKAAA
                     AYTIGKRVA"
     gene            582..1253
                     /locus_tag="PLI76_10635"
     CDS             582..1253
                     /locus_tag="PLI76_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HOI62529.1"
                     /translation="MIRIVNLRHRIVDIPDLAVDARHVAVIGPNGSGKTTLLSICSGI
                     EEPRRGTVRLLGRAPAAVKIGWVGEFPDRTLLFSRAYDEIASSPRFRHRPCLETDERV
                     RAAAGTIGIPHLLDAKVSALSGGEKALVALAAAVVDDPEVLILDEADSHLDGGTAARI
                     QAAVKKSTAAHVLWCTQSMDTAAEADYVVFMAGGAVRHHGTPEEVFTSLEETCFYPTM
                     WRIRR"
     gene            1250..1948
                     /locus_tag="PLI76_10640"
     CDS             1250..1948
                     /locus_tag="PLI76_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOI62530.1"
                     /translation="MRVELEDLLLSRGPFTLRGSGTFEEGVHLVSGAVGSGKSTLALL
                     LAGLLHPGSGAIRRQRVSSTLLLLQFPEYHVTSPTVAEEAGSWGLVPDDVLARADLAD
                     RGDDDPFHLSRGELKRLILACTFMRDPDLLMLDEPFSSLDCAAKQKACRMIEERGRGI
                     TIIFSHERAVLPRVDAIWEMEGGILTALGNVPGAIPRWRHAPPYLAYALDRGARPENI
                     RLEDAREAICRIQG"
     gene            1930..2583
                     /locus_tag="PLI76_10645"
     CDS             1930..2583
                     /locus_tag="PLI76_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62531.1"
                     /translation="MQDPRLRLLSTVVLSLAAFASTMGAVVALVWWLVFTPRTKALPR
                     PGVLFSLTAMIALTALVSAWGGGPGLSYFIRMVVVLLLAAWAYTETEEGEVLAVAVWA
                     LGKRIGFEIGLIAEMGLTGLLVIRQEIDQARVALALKGIPIGARSIVPIAVLLIVTQI
                     RRADEVARLLVVRGYALGGRICPRFRTDPRDILASVSAVILGLLSCFPVRDVFILLG"
     gene            2637..>3336
                     /locus_tag="PLI76_10650"
     CDS             2637..>3336
                     /locus_tag="PLI76_10650"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HOI62532.1"
                     /translation="MCAAKFDTLHITDTTLRDAHQSLIATRLRTEDMLPLARAIDEVG
                     FFSVEAWGGATFDSCIRFLNDDPWERLRMLKAELKRTPIQMLLRGQNLVGYRHYPDDV
                     VEKFVEASARNGVDIFRVFDALNDIRNMKKAMEEVQNVGAHLQGAISYTTSPVHSVAS
                     FIDMAGELYSLGCNSICIKDMAGLIMPHDARDLISGIKETMDVMVNLHSHCTSGVAPL
                     SYQAAIDAGVDILDT"
BASE COUNT          574 a         1048 c         1036 g          678 t
ORIGIN      
        1 ctagcattcc gggtcatgcc ggctcccata acttttgggt tgacatgatt tttattgtca
       61 accatctaaa ataagatggt ttacatgaaa caccgcgccc agatccttgc ttacagcggc
      121 acattcgttg cgctcatcgc tgccgggagc tggatatcca taccgctgcc cccggttccg
      181 atcacgctcc agaccctctt tgtgctcctc gccgggatcg tcatgaaacg ctacgcggtg
      241 atccccgtcg ccctctacgt cctcctcggg gcgatgagcc tcccggtctt tcataacggc
      301 acggcaggcg tcggtgtcct cctcggcccc acgggagggt tcctgatcgg ttttatcccc
      361 gccgccctca tcgccgggct cgcctatgag caggaatcac cgaaatggcg tatcggaggc
      421 ctgattgcgg cctgtgcaat ctacctctcg gccggcgccg tatggctctc atactctgcc
      481 tcgatcccgc ttctccaggc gatcttgatc ggtgtcgctc cattcatcgt cggtgatacc
      541 ctgaaggcag ccgccgcata taccatagga aaacgggtgg catgatccgg atcgtcaacc
      601 tccggcaccg gatcgtcgac attccggatc tggcggtgga tgcacggcat gtcgccgtca
      661 tcgggccgaa cgggagtggg aagacaacgc tcctctcgat ctgctccggt atcgaagagc
      721 cccgaagggg aacggtccgg ctcctcggga gggcgcccgc cgccgtgaag atcgggtggg
      781 tgggagagtt tcccgaccgg acgctcctct tctcccgggc atacgacgag attgcatcgt
      841 ctccccggtt ccgccaccgc ccctgcctcg aaaccgacga acgtgtccgg gcggccgccg
      901 ggacgatcgg catcccgcac ctcctcgatg cgaaggtatc ggccctctcg ggaggcgaga
      961 aagccctcgt cgcgctggct gcggccgtcg tcgacgatcc cgaggtgctc atactggacg
     1021 aggccgactc gcacctggac ggcgggacgg ccgcgcgcat acaggccgcc gtgaagaaga
     1081 gcacggcggc ccatgtgctc tggtgcacgc agtcgatgga caccgcggcc gaagcagatt
     1141 atgtcgtctt catggcaggg ggggcggtcc ggcaccacgg cactcccgag gaggttttta
     1201 cgagcctcga ggagacgtgt ttctacccca cgatgtggag gatccgccgg tgagggtgga
     1261 acttgaggat cttctcctct ctcgcggccc gttcaccctc cggggcagcg gcaccttcga
     1321 ggagggcgtg cacctggtca gcggagcggt ggggagcggg aaatcgacgc tcgccctcct
     1381 gcttgccggg ctcctccatc cggggagcgg tgcgatccga cggcagaggg tctcatcgac
     1441 cctgcttctg ctgcaatttc ccgaatatca cgtcacctcg ccgaccgttg ccgaagaggc
     1501 cggatcctgg gggttagtgc cggacgatgt gctcgcccgg gcggatctcg ccgaccgcgg
     1561 cgacgacgac ccgttccacc tctcccgcgg tgaactgaag cggttgatcc tggcctgcac
     1621 gttcatgcgg gatccggacc tgctgatgct ggacgaaccg ttcagttcgc ttgattgtgc
     1681 cgcgaaacag aaggcctgca ggatgatcga ggagaggggg cgggggatca ccatcatctt
     1741 ctcgcacgag cgcgcggttc tcccccgggt ggacgccatc tgggagatgg agggcgggat
     1801 cctgaccgcc ctcggcaacg tgcccggcgc aatcccgcgg tggcggcacg ccccacccta
     1861 ccttgcgtat gccctcgacc ggggcgcccg cccggagaac atcaggcttg aggatgcacg
     1921 ggaggcgata tgcaggatcc aaggctgagg cttctctcca ccgtggttct ctcgcttgcc
     1981 gcgtttgcaa gcacgatggg cgccgtcgtc gccctggtct ggtggctcgt cttcacgccg
     2041 cgaacaaaag ctctcccacg gcccggagta ctcttctccc tcaccgctat gatcgcccta
     2101 accgcactcg tctcggcatg ggggggcggc cccggcctct cctacttcat ccggatggtc
     2161 gtggtcctcc tccttgccgc gtgggcatat accgagacgg aggagggaga agtgcttgct
     2221 gtggcggtct gggcgctcgg gaagcggatc ggatttgaga tcgggctcat cgccgagatg
     2281 gggcttaccg ggctcctcgt gatccggcag gagatcgacc aggcgcgggt cgcacttgcg
     2341 ctcaagggga tcccgatcgg cgcacgctcg atcgtgccga tcgccgttct gctcatcgtc
     2401 acccagatcc ggagggccga cgaagtcgcc agactgctgg tagtacgggg atacgcgctc
     2461 ggagggcgga tatgcccccg gttcagaacg gacccgcgag acattctggc atccgtatcg
     2521 gcggtaatac tcgggctttt atcctgcttc cctgttcgtg atgtttttat attattaggg
     2581 tgaatttttt agaggcttta tcgctatctc ctcagccctt cgaggtgcaa aattcgatgt
     2641 gtgctgccaa gtttgataca ctccacatca ccgataccac gcttagggat gcgcatcagt
     2701 cgctgatcgc cacccggctc cggactgagg atatgctccc tcttgcccgg gccattgacg
     2761 aagtcggctt cttctcggtc gaggcctggg gcggggcgac ctttgacagt tgcatcaggt
     2821 tcttgaatga cgacccctgg gagcgtctcc ggatgctgaa ggccgagctc aagcgcaccc
     2881 caatccagat gctcctgcgg ggccagaacc tcgtgggata ccggcattat cccgacgatg
     2941 tcgtggagaa gttcgtggaa gcatcggcaa ggaacggtgt cgatatcttc cgggtctttg
     3001 atgcgttgaa cgatatccgg aatatgaaga aggcgatgga ggaggtgcag aacgtcgggg
     3061 cgcacctgca gggcgcgata tcctatacga caagcccggt tcactccgtc gcatcgttca
     3121 tcgatatggc cggggaactc tactcgcttg ggtgcaactc aatctgcatc aaggatatgg
     3181 ccggcctgat catgcctcac gatgcccgcg acctgatatc cggcatcaag gagacgatgg
     3241 acgtcatggt caacctccac tcccattgca cgagcggcgt tgcgcccctg agttaccagg
     3301 cggcgatcga tgcgggtgtg gatattctcg atacgg
//
LOCUS       DAOSEE010000193         3185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_10731_length_3185_cov_181.945367, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000193 DAOSEE010000000
VERSION     DAOSEE010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10731_length_3185_cov_181.945367"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(629..2218)
                     /locus_tag="PLI76_10655"
     CDS             complement(629..2218)
                     /locus_tag="PLI76_10655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOI62533.1"
                     /translation="MISVLYVDDEPALLELTRLFLERYGNYSVDTAISALDALDMMKA
                     APYDAIISDYQMPVMDGIALLKHLRAEDSSTPFILFTGRGREEIAIEALNAGADFYLQ
                     KGGEPGSLFAELEHKLRLAVERRRIADELSESRQRMTAIVSHLPDATFAIDLEGKVIV
                     WNRAMEEMTGVKEEEILGTGDHACAAAIYRARRPILLDFIIGREEKIKEYYPDLTVKD
                     DRLITENYIPTLYGGRGAHVWLVASPLYDAHGTITGAIESIRDITDRKLAEEALHRSE
                     EKYRTLTEQTNDIIYLMDVNGRLTHIGPQVARYGYTPEEIISQNLSVFVVEEDVAIVV
                     DDLEKAIATGIPRYTVFRARDKSGNIYWFEDSGAPVFDENGVIVGVSGALRDITDRRR
                     IEQKLVESERIYRSILENMRDVFYRSDREGILIMVSPSGAELLGYGSVEELLGKKIDS
                     TLYADRGEQDRFLAEMDKRGEVVNYRVILKRRDGSPIPVETSSRRYYDENGCCLGIEG
                     FFRSAPGPGFRGETSGEYSRC"
     gene            complement(2638..3057)
                     /locus_tag="PLI76_10660"
     CDS             complement(2638..3057)
                     /locus_tag="PLI76_10660"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOI62534.1"
                     /translation="MSPASTLSAPGEARRPPPRPRPGVWEPLRADDLWRPESALRRGP
                     AGRPCPHPATGICVGYCITSISPEHVGKIEPLFIGEAYRSRGNGTTFMNRAPAWLDGN
                     GSIENRASVADGNEEAFSSYRRFGFYPRRAVLEQKRE"
     gene            2997..>3185
                     /locus_tag="PLI76_10665"
     CDS             2997..>3185
                     /locus_tag="PLI76_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62535.1"
                     /translation="MGAVVGVEPLPGRITSTPAISVVQRSIGGAGALMPLLPPSMPGT
                     LWVVTLIACAARVQDTYAV"
BASE COUNT          562 a         1029 c          933 g          661 t
ORIGIN      
        1 gcggaaccgg acggagtctt ctgctatacc ttcttcaagg cgaccgggag aagatagccg
       61 gcgccatgag ggagcccccg aatgccatga gccagaagag cgcggggtag atgatcggtc
      121 tctccagtcc gccggggcca tgaaggctcg cgataccgat atagctcaac ggcgtacgcc
      181 gccaggaaga cgaggccgag aacgccgatc gcatccgtgt tgatgctgta gccgggcacc
      241 agggccgcgc ccgccatgag cgcagtcatg aaccgggcgg gggcaagaac agcacccctg
      301 caaccgttcg gttgccgtct accgggtttg gtccgtgcat ggcccggcga cggcgcttca
      361 tctactatat tcctgcaccg gtattgcttt gccgccatca cctgcaggag cggtacggcc
      421 cccggcccta tccccggaac ggcgagcaaa acccgcccat ctccttatcc acggagatcc
      481 cgctcttcag gcagcgggcg gttcaccgga tacccgccgg catgcgatgc gccgaagacc
      541 cgtccttcgc gagggtcgac cccccgggct cacgcgtggc tctcacccgg ttgcagcagg
      601 ctctctcaag gtgcaatcgg tggagacttc aacaccgtga gtattcgccg gaggtttcgc
      661 ctctgaatcc gggtccgggg gcactcctga agaacccctc tatgcccagg cagcacccgt
      721 tctcgtcgta gtacctgcgg ctgctcgtct ccacggggat cgggcttccg tcacgccgct
      781 tcaagataac cctgtaattg acgacctctc cccgtttatc catctcggca aggaacctat
      841 cctgctctcc ccggtctgca tacaacgtcg agtcgatctt ctttccgagc agttcctcga
      901 ctgaaccgta tcccaggagc tctgccccgg aggggctcac catgatgagg atgccttcgc
      961 ggtcgctccg gtaaaagacg tcccgcatgt tctccaggat cgatcggtag atcctctcgc
     1021 tctcaaccag cttctgctcg atccttctcc ggtcggtgat atcccgcagg gcccccgata
     1081 ccccgacaat gacaccgttt tcatcaaaga ccggtgcgcc gctatcctca aaccagtaga
     1141 tgttgccgga tttatcccgc gcgcggaata cggtatacct gggtattccc gtcgctatgg
     1201 ccttctcgag atcatcaacc acgattgcga catcttcctc gacaacaaac acggaaaggt
     1261 tctgcgatat gatctcctcc ggagtatacc cgtacctggc aacctgaggg ccgatgtggg
     1321 tgagtcgacc gttgacgtcc atcaggtaga tgatgtcgtt tgtctgttcc gtcagggtcc
     1381 ggtacttctc ctcgctcctg tggagcgcct cctctgccag tttacggtcg gtgatgtcgc
     1441 ggatggactc gatcgccccg gtgatggtgc cgtgcgcgtc gtagagcggg gatgcgacaa
     1501 gccatacatg agcacctctt cctccgtaga gggtggggat gtagttctcc gtgatcaacc
     1561 tgtcgtcttt tacggtgagg tcggggtaat actctttgat cttctcctcc ctccctatga
     1621 tgaagtcgag gaggatcggc cgtctcgccc tgtagatcgc ggcggcacac gcgtggtccc
     1681 cggtccccag gatctcctcc tcttttacac cggtcatctc ttccatcgcg cggttccata
     1741 cgatgacctt cccctcaaga tcgatcgcga aggtggcatc cggcaggtgg ctgacgatgg
     1801 cggtcattcg ctgccgggac tcgctgagtt catccgcaat ccgccgccgc tcgaccgcca
     1861 ggcggagctt atgctccagt tccgcgaaca acgaccccgg ttcgccgcct ttctgaagat
     1921 agaagtctgc accggcgttg agggcctcta tcgcgatctc ctcacgcccg cggccggtga
     1981 acaggatgaa cggggtgctg ctgtcctcgg cccgcaggtg tttgaggagc gcgatgccgt
     2041 ccatcacggg catctggtaa tcggagatga tggcatcgta gggggctgcc ttcatcatgt
     2101 cgagggcatc gagtgcagat atcgcagtat cgactgagta gttcccatac cgctccagga
     2161 atagcctggt cagttccagg agagcgggtt cgtcatcgac gtaaaggact gatatcacga
     2221 tggtcccccg gtgttgccga cggcccgata tggatagtcg gttatgtcta catgtcgcag
     2281 tggcatatat aagcttatcc cttcctgctc tccctcacac gtgctcttac gagagaatgt
     2341 ggtgaagcag gatgggttct ggcatatacc ggtaagatac gatggccgtc tcctcccgcc
     2401 atgcggccga tgcgaccacc atggccggag ccccggtcag gcccggccgg tgttctccga
     2461 atgactttcc cggctccctt ctcccggagg cacaggagcg gtgggtcccc gttgccgcca
     2521 gaatcgcgat tgaccggtga tgcccggcac gccacgggaa gatgccctcc tgcagcgccg
     2581 gaactcctct cgctcccatg tggcgatgaa ctctctctgg atgtagcggc ctccacgcta
     2641 ctccctcttc tgttcgagca ccgcccggcg ggggtagaac ccgaaccgcc ggtaggacga
     2701 gaacgcctcc tcgttcccgt cagcgaccga ggcccggttc tcaatcgaac cgttcccgtc
     2761 cagccacgca ggcgcccggt tcatgaaggt cgtgccgttc ccccgcgagc ggtatgcctc
     2821 gccgataaag agaggctcga tcttgcccac atgctccggg gagatcgagg ttatgcagta
     2881 gcccacgcag atccccgtag ccggatgagg gcagggtcga cccgcgggac cccggcgaag
     2941 tgcgctttcc ggtcgccaaa ggtcgtctgc tcgtaacggc tcccaaacgc ccgggcgtgg
     3001 gcgcggtggt gggcgtcgag cctctcccgg ggcggataac gtcgacgccg gcgatatccg
     3061 tggtgcagcg atcgataggg ggtgccggtg cgctcatgcc tcttctcccc ccatcgatgc
     3121 ccgggaccct atgggtcgtc accttaattg catgcgcggc gagagtacag gatacgtatg
     3181 ccgtg
//
LOCUS       DAOSEE010000194         3183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_10739_length_3183_cov_251.115090, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000194 DAOSEE010000000
VERSION     DAOSEE010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10739_length_3183_cov_251.115090"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PLI76_10670"
     CDS             <1..299
                     /locus_tag="PLI76_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62536.1"
                     /translation="GGLGYDAGYRPLFALRQGEIIPLKSVAFNTAEPWYSIDVSHGTK
                     DGSGLVGTHYCTIFTLRDLTRAERVYCPDGELFWPPPGSSPAGVDGLSGLGVVS"
     gene            339..734
                     /locus_tag="PLI76_10675"
     CDS             339..734
                     /locus_tag="PLI76_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62537.1"
                     /translation="MESDNKRLIAVGLLAFIGVVVLVAAVVFGFTTLITLVTGVDGPP
                     QMLVVEVVGEEALQNASVVHLTDRDLKQHPVLATAIREAGSDSGVSASAPMTGVECLA
                     LTESFGVYTRDAPILEYDGVYYSTRVLLH"
     gene            755..1195
                     /locus_tag="PLI76_10680"
     CDS             755..1195
                     /locus_tag="PLI76_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62538.1"
                     /translation="MEEGKKSGNRLGTAGFVIACALAGIVAVALGLVLADALLIYAME
                     GSGGQPMLYVMESAEPGENVVIPLTEKDFADHPALDAVIRGDERNPSAWEWGRSDRRF
                     IGGINVSYVESRVLQDTYGPDRETWVYPHLEYEGAYYLVVTTWP"
     gene            1215..2315
                     /locus_tag="PLI76_10685"
     CDS             1215..2315
                     /locus_tag="PLI76_10685"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOI62539.1"
                     /translation="MNRAIGTWALLLALLLAIAPAAAADLGPNQHLIPELRVNESIEA
                     IVISAELSLQPEWDGSVTTGIPFGAVMVHTANATTLIFDRDGNHLFSIDDERSAKIPT
                     PAGVEKPCTRVHPLPNDSRVYHHGGVTFVFGTAGEPPILTIIDESPKPDEEPITIEIT
                     SPNEGGVAWIDVAPAHVAVAGEVHAPAGVHSVVVRSGSVEVSCGNAAEFACSVPVSFG
                     KNTITVVATDNRRHRAEKTVNLTVRSGIPPPLIAVSGRVTDAGGSPVPGALVRLESVF
                     DLGGEPIAAMTVTGEDGRYLVEDALGYRQTVTVEKEGYSPHGWEVVFENLTNTLDLEL
                     EPASRPVPGFGPAVGVLGLLGARWILRNTKRQ"
     gene            2473..3072
                     /locus_tag="PLI76_10690"
     CDS             2473..3072
                     /locus_tag="PLI76_10690"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOI62540.1"
                     /translation="MSYSEPSTDTILGIRMYETERLLLKVATPDDVAAIVSYLIRNRD
                     FLMETEPPRDDRYYSFEYQAGEILHERKMMENGQLLKLWIVKKENPTQMIGTVSFNTI
                     IRGPFQSCFLGYRLDYAEVRRGYMTEALREAIRVLFGECRLHRVEANILPGNGPSLRV
                     VEKLGFHYEGLALRYLLVNGKWEDHIRMVLLNDRWAGDR"
BASE COUNT          602 a          937 c         1033 g          611 t
ORIGIN      
        1 cgggaggcct ggggtatgac gccggatatc gacccctctt tgccctgcgc cagggggaga
       61 tcatcccctt aaagagcgta gcgttcaaca cggcggagcc ctggtacagc atcgacgtct
      121 cgcatggaac aaaggacggt tcaggactgg tgggtaccca ctactgcacg atctttacgc
      181 tccgcgacct taccagggcc gagagggtgt actgcccgga cggggaactt ttctggccgc
      241 cgcccggttc gtccccggca ggtgtcgatg ggttatccgg attgggggtc gtctcttgat
      301 gccggacggc cggttcactc cggagacagg gtggccgcat ggagtctgac aataagcgcc
      361 tgatcgccgt aggacttctc gccttcatcg gcgtcgtcgt cctcgtcgcg gcggtcgtgt
      421 tcggatttac gacccttatt accctggtca cgggagtcga cgggcctcct cagatgttgg
      481 ttgtggaggt ggtcggggaa gaggccctgc agaacgcatc tgttgtccac ctgaccgacc
      541 gggacctgaa gcagcacccg gtgcttgcca ccgcgatccg ggaggccggc agcgattcgg
      601 gcgtatccgc gtcggccccg atgacggggg tcgagtgcct cgccctcacc gagtccttcg
      661 gcgtatacac aagggatgcg ccgatcctcg aatacgacgg agtatactac tccacccgcg
      721 ttctgctcca ttagaacgct tgaaggggtg cagaatggaa gaaggtaaga aatccggcaa
      781 ccggctcgga acggcgggat tcgtcatcgc ctgcgccctt gccgggatcg tcgcggtcgc
      841 gctcggcctc gtgctcgccg acgctctcct catctatgcg atggagggct cgggcggtca
      901 gccgatgctc tacgtcatgg agagcgcgga gccgggagaa aacgtcgtca tccctctgac
      961 cgagaaggac tttgccgacc acccggcgct cgatgcggtc atccgcggcg atgagcgaaa
     1021 cccgtcggcg tgggagtggg gtaggagcga ccggcgcttc atcgggggaa ttaacgtctc
     1081 ctacgtggag agcagggtgc tccaggacac ctacggcccc gaccgggaga cgtgggtcta
     1141 cccgcacctg gagtacgaag gggcgtacta cctggtggtg acgacatggc cgtgagagag
     1201 ccgggggagg aagaatgaac agagcgatcg gaacatgggc actcctcctc gcgctgctgc
     1261 tcgcgatcgc cccggcggcg gccgcggatc tcggtcccaa ccagcacctg atcccggaac
     1321 tgagggtgaa cgagtcgatc gaggcgatcg tcatctccgc ggagttgagc cttcagccgg
     1381 aatgggacgg gagcgtgacc accgggatac cgttcggcgc ggtcatggtc catacggcca
     1441 atgcgacgac gctgatcttc gatcgcgacg gaaaccacct cttctccatc gatgacgaac
     1501 gttccgcaaa aatccccacc cccgcaggcg tcgaaaagcc ctgcacccgg gtgcacccgc
     1561 tcccgaacga ctcccgggtc tatcaccacg gcggcgtgac cttcgtcttc ggcacggccg
     1621 gggaaccccc catcctcacc atcatcgacg agagcccgaa gcccgacgag gagccgatca
     1681 ccatcgagat cacctccccg aatgagggcg gcgtggcctg gatcgacgtc gccccggccc
     1741 atgtcgccgt cgccggggag gtgcatgccc ctgcgggagt gcacagcgtg gttgtccgga
     1801 gcggttcggt ggaggtctcg tgcgggaacg cagcggagtt cgcctgctcg gtgccggtct
     1861 ctttcgggaa gaacacgatc accgtcgtgg cgacggataa ccgcaggcac cgggccgaga
     1921 agaccgtgaa cctgaccgtc cgtagcggca tcccgccgcc attgatcgcc gtctcgggca
     1981 gggtgacgga cgccggcggc agtccggttc ccggcgcact ggtgcggctc gagtcggtgt
     2041 ttgatctggg cggcgaaccc atcgcagcaa tgaccgtgac gggggaagac ggccgctacc
     2101 tggtagaaga tgccctcggc taccggcaaa cggtcacggt cgagaaggag ggttactcgc
     2161 ctcacgggtg ggaggtcgtc ttcgagaacc tgacgaacac cctcgacctg gagctggagc
     2221 cggcgagccg tccggtcccg gggttcggtc ccgcggtagg tgtacttggt ctccttggag
     2281 cacgatggat cctccggaac acgaagagac agtgaaatga tccggcagca tgccggtgtg
     2341 tctggagaat aaaccgtgtt cacctgggaa tttgggcttg gcgtcgggct aaattcatgt
     2401 atgcaggggc caaatggtag agaccgcatt gtttgtgatc tcccttttgc ttttccggga
     2461 gaaggagggg ccatgagtta ttctgaacca tccacagaca ccatcctggg gataaggatg
     2521 tatgagacag agcggttgct gttgaaggtt gcaacgcccg acgatgtcgc tgcgattgtc
     2581 tcgtacctta tccggaaccg tgactttctc atggagacgg aaccgccccg ggacgaccga
     2641 tactattcgt tcgagtatca ggcaggcgag atcctgcacg agcgaaagat gatggagaat
     2701 gggcagttac tcaagctctg gattgtgaag aaggagaacc cgacccagat gatcggcacc
     2761 gtcagcttca acaccatcat cagaggtccg ttccagtcct gcttccttgg gtatcgtctg
     2821 gactatgccg aggtacgccg gggatacatg accgaggcgt tgagggaggc gatacgggtc
     2881 ttgttcgggg agtgccgcct gcatagggtt gaagcaaata tcctccccgg caacggccct
     2941 tctctgcgag ttgtagagaa actcggattt cattatgagg gcctcgcact ccggtacctt
     3001 ttggtgaacg gaaagtggga ggaccatatc cggatggtgc tgttaaacga caggtgggct
     3061 ggagatagat gaggttccgt ttgcccgctt ccagcggcat ggctcatcct gtcactccct
     3121 aaactctctt tgctatcgtc tctatcgcct cgatgatcgt ccgttgctgc ggcttcatga
     3181 tcg
//
LOCUS       DAOSEE010000195         3136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_10962_length_3136_cov_212.793249, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000195 DAOSEE010000000
VERSION     DAOSEE010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10962_length_3136_cov_212.793249"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="PLI76_10695"
     CDS             complement(<1..1009)
                     /locus_tag="PLI76_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOI62541.1"
                     /translation="MSFIPRYLICTALCACILIAGCSVPGGREPDIISGTTGDLAVHF
                     IDVGQGDSVLLQFRDKTMLIDAGERGMGGRVVTYLENLGVERLDVVVATHAHSDHIGG
                     LATVISAFPVGRFVDAGQAHPTATYENLLVLVEEKGIPYTAAVRGQTIALDPDLDIRV
                     LNPAAQPLGDVNEDSVVLKATYGDISYLFTGDAGKPAEESMAKAGLDLDADVLKVGHH
                     ASRYASSAEFLSAVSPAVSVILVGEGNDYGHPHEEALERLEAAGSRIYRTDRDGNVVV
                     TTDGQKIAVTAGGAPAATVTVTQTTSAVTPAATTSTVTPVVTAPPTGVTITYLSLLDE
                     MV"
     gene            1149..1970
                     /locus_tag="PLI76_10700"
     CDS             1149..1970
                     /locus_tag="PLI76_10700"
                     /inference="COORDINATES: protein motif:HMM:NF019949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1724 domain-containing protein"
                     /protein_id="HOI62542.1"
                     /translation="MSALEVYGEFHDDIQVIFRSRLVTQVLLALGSEKRSLSTLRDIT
                     GSSSQALIPKIRQLEALQYVEQVRDGYTLTPIGRLVEQEIERLVTMMGVIERHKDFWA
                     AHDIESIPRRFLNELHDLYDAEVVRDVEEDVFAVYRVFGTIIERASWIHGVSSIMSPV
                     HAGAIREAALQGKPVELVVSQELAGKLVEEPYRTMLESLRGHAGFGLYVAASPVRLGM
                     AVTDGFVSLGLYRRDKGTYDTATDLVSTDAAAIAWGERVFQHYRATADRLHLPSM"
     gene            2128..3027
                     /locus_tag="PLI76_10705"
     CDS             2128..3027
                     /locus_tag="PLI76_10705"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOI62543.1"
                     /translation="MFASINRAGAIRPFCPAVTRFIVYTSLYLSIAGAAMVYISCFLQ
                     GLAFDPVAAALLVLVTFAVYNLNRRTDEDEDAINHTERYAFTKKHGLILFRSAACAYA
                     VALGLSALWGPASLIVTAVPLVAGIVYSVPLFPARFGFKRLKEVPLMKSMVVAFSWAV
                     PPALLPICHAALPAGAATGIVGAFFFLLVFTNTVVFDIRDVEGDAVSGVRTIPTIFGA
                     GGALLLLTGLNAGFGTALLLAGRTLPGSCITPALAAGVVYAQGYILCFRRLVRETLLF
                     ELLTDGQFIVLAGIFSLSSLVAP"
BASE COUNT          522 a          997 c         1009 g          608 t
ORIGIN      
        1 tgaccatctc atccaggagg ctgaggtagg tgatggtgac gccggtgggg ggagcggtca
       61 cgaccggggt cactgttgag gtcgttgccg ccggggttac tgcggaggtc gtctgcgtga
      121 ccgtcacggt cgccgccggg gctccgccgg ccgtaacggc gatcttctgg ccgtcggtcg
      181 tgacgacgac gttcccatcg cggtcggtcc ggtagatccg cgacccggcg gcttcgaggc
      241 gctcgagagc ctcctcgtgg gggtggccgt agtcgtttcc ttcgccgacg agtatgacgc
      301 tgaccgcggg gctgaccgcg gagaggaact cggcggagga tgcgtaccgg ctcgcgtggt
      361 ggccgacctt caggacgtcg gcatcgaggt cgagcccggc ctttgccatg ctctcctccg
      421 ccggtttccc ggcatcgccc gtgaagagat aggatatatc cccgtacgtc gccttcagga
      481 cgaccgaatc ctcgtttacg tccccgaggg gctgtgcggc cgggttcaag acccggatat
      541 cgaggtcggg gtcgagggcg atcgtctgcc cgcgcactgc cgccgtatac ggtatcccct
      601 tctcctcgac caggacgagc aggttctcgt acgtcgcggt ggggtgcgcc tgcccggcgt
      661 cgacaaaccg gccgaccggg aaggcggata tgaccgtggc aagtcctccg atgtgatcgg
      721 agtgggcatg ggtcgccacc accacgtcga gacgttctac gccgagattc tcaaggtacg
      781 tgacgacgcg cccccccatg ccgcgttcgc cggcgtcgat gagcatggtc ttatcgcgga
      841 actggaggag gaccgagtcg ccctgcccga cgtcgatgaa gtggacggca aggtccccgg
      901 tggtcccgga gatgatatcg ggttctcgcc cgccgggaac ggagcagccg gcgatgagga
      961 tgcaggcaca gagtgcggtg cagatcaggt atctgggtat gaacgacatg ctgcctgtag
     1021 aggtatcggg ttcccggcta taagtgcgct ggggtcggat tgggtggcac gtcgggggag
     1081 ggagagggcg tgcaggaatg aaaagagtga tagcgccggc cggcgcttct taattataag
     1141 gcgatggtat gtccgctctt gaagtctacg gcgagttcca cgacgatatt caggtgatct
     1201 tccggtcgag gctggtaacg caggtgctgc ttgccctcgg gagcgaaaag aggtccctct
     1261 cgaccctccg cgacatcacg gggagttcgt cgcaggccct catcccgaag atccggcagc
     1321 ttgaggccct gcagtacgtc gagcaggtgc gggacgggta taccctcacc cctatcggca
     1381 ggctcgtcga gcaggagatc gagcgcctgg tgacgatgat gggcgtgata gagcgccaca
     1441 aggacttctg ggccgcacac gatatcgaga gcataccccg gaggttcctg aacgaactgc
     1501 atgacctcta cgacgcggag gtggtcaggg acgtcgagga ggacgtcttt gccgtataca
     1561 gggtgttcgg gaccataatc gagcgcgcgt cgtggattca cggggtatcc tcgatcatga
     1621 gcccggtgca tgcgggtgcg atcagagagg cggctctgca gggcaaaccc gtcgaactcg
     1681 tcgtctcgca ggagctcgcc gggaaactgg tagaagagcc gtaccggacc atgctcgagt
     1741 ccctccgggg gcatgccggt ttcgggctct acgtcgccgc ctcgccggtt cgcctcggga
     1801 tggcagtgac cgacggcttc gtctcgctcg gcctctatcg gcgcgataag ggaacctacg
     1861 acaccgccac ggacctcgtc agcaccgatg cggcagccat cgcctgggga gaacgggtgt
     1921 tccagcatta ccgggcaacc gcagaccggc tccatctgcc ctcgatgtag tactcgtttg
     1981 actccagccg gagcccgcgc cccggggatc acccacaacc gaccggatgg aggggtgccg
     2041 ccctgccgga gcagcgcgcc cgtgttaacc agaccgatga atattcctcg gtacaatgaa
     2101 tattcacctt tctcctgaca actatgcatg ttcgcctcga tcaaccgagc cggggcaatc
     2161 cgaccgttct gtccggccgt tacccggttc atcgtataca cctcgctcta tctctccatc
     2221 gcgggggctg cgatggtgta catctcctgt tttctgcagg gtcttgcttt cgacccggtc
     2281 gccgccgcac tactggtgct cgtcacgttt gccgtttata atctcaaccg caggaccgac
     2341 gaggacgaag acgccataaa ccacaccgaa cgctatgcct tcacgaagaa acacgggctc
     2401 atcctctttc gctctgcggc ctgcgcctac gccgtcgccc tcggtctctc cgccctttgg
     2461 gggcccgcaa gcctcatcgt gacggccgtt cccctggtcg ccgggattgt ctacagcgtg
     2521 ccgctctttc ccgcccggtt cgggttcaaa aggctcaagg aggtccccct catgaagagc
     2581 atggtcgtgg cattctcctg ggcagttcct cccgccctcc tgccgatctg ccatgccgcc
     2641 cttcccgcag gcgccgccac cggtatcgtc ggcgccttct tcttcctcct ggtcttcacg
     2701 aacacggtgg tcttcgatat ccgggacgta gagggagacg cggtatcggg cgtgagaacg
     2761 atcccgacga tcttcggcgc cgggggagcg ctccttctcc tcacgggatt gaacgcaggc
     2821 ttcgggaccg cgctgctgct cgccggcagg acgctgccgg gctcctgcat aacgccggcg
     2881 ctggccgcag gcgtggtcta tgcccaggga tacatcctct gcttccggag gctcgtgcgg
     2941 gagacgctcc tcttcgaact gctcaccgac gggcaattca tcgtgctcgc cggcatcttc
     3001 tccctctcct ccctcgtagc gccgtagctt ccggatccgg cgggggcacc cgggaccgga
     3061 gtgtcgcctg ccccccgccc acgcggagcc tccacaaatt tcatcgaacc ggtgaatgtt
     3121 cgtggacata acgaat
//
LOCUS       DAOSEE010000196         3117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_11060_length_3117_cov_239.054540, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000196 DAOSEE010000000
VERSION     DAOSEE010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11060_length_3117_cov_239.054540"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="PLI76_10710"
     CDS             <1..1023
                     /locus_tag="PLI76_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HOI62544.1"
                     /translation="GGNFGRLLVQWVLTYIQQRIGAETGVKPGAEMLVAIEEAEKHQK
                     PVAFIDRDIRITLGRFWGRMGIWEKIKLIGALIYSLVSVEGQDLDVDELTNQDVVSAA
                     MEEFRKFSPHGAQALIDERDAYLAHQILMLGNRYDRVLAVVGAGHIQGVQRYLNAPET
                     LPPLPSLTADVKGLPWGKIIGAAVTIIFLLLIAAIAFSGVGLEVLIAALVYWILINGV
                     LSAAFTLLAGGHPFSAATAFAVSWLTSLNPLLAAGWFAAIVEAKIRKPTTGELRQIIE
                     AETFSEMRRIPLFRVVLVAALANVGSSLGTVAYFVFIFPVLGIDPTVVITEGFSNMIR
                     MIAGFF"
     gene            1086..1616
                     /locus_tag="PLI76_10715"
     CDS             1086..1616
                     /locus_tag="PLI76_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOI62545.1"
                     /translation="MSPIGGTVNLGVTVIRSRHSVRKYKESPIEDKAVKDALDCARLA
                     PTARNEQPWLFGTIRDRDMLKAIADLAENARFIADAPICFAVFGKKDAKYYLEDCCAA
                     TTQLILALQAWGVGTCWVAGEKKDYADGVRRLLNVPDEYALVSLVPAGYPEEIQIKKK
                     KLPDEVTFFERYEEEK"
     gene            complement(1718..2272)
                     /locus_tag="PLI76_10720"
     CDS             complement(1718..2272)
                     /locus_tag="PLI76_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2115 family protein"
                     /protein_id="HOI62546.1"
                     /translation="MSRGEDGVDIRRVCARLRSATTRAELASTLYREVSGYSLFDLQA
                     LRGRVERDLQSVPAGYRQRLYPLMMEMLFATHHTLIATMRRPDPDLPDEPLAGEFQEF
                     CGMVERTCLTRESDQRLGLLYYLLAAFNIFVLALPAHPVGTPFPGGLTVEIRNGEYLC
                     PVREKADDVENALCPYCPAKQSDV"
     gene            complement(2379..>3117)
                     /locus_tag="PLI76_10725"
     CDS             complement(2379..>3117)
                     /locus_tag="PLI76_10725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HOI62547.1"
                     /translation="PTIRIHPGIIAQAAATAAAMMPGRFVLGLGSGENLNEHIFGDRW
                     PPAPVRIEMLEEAVEVIRALWTGSMQTYYGSFYTLENAQVFSLPESLPPIAIASEGPI
                     SASLAARVGDGFINGGTNAERSLVVFKKSGGDDKPAYVEISVCWAESEEVGERTAYEE
                     WPIAANTGELNRLLPTPAHYEQLAKMATLEDVVEGMPCGPDPEPYIKKIEDRVRMGFD
                     HICIHQIGEQQKEFMDFAAAEILPHFR"
BASE COUNT          538 a          992 c          995 g          592 t
ORIGIN      
        1 ggcgggaact tcggccggct cctcgtccag tgggtgctca cctacatcca gcagcggatc
       61 ggcgccgaga cgggcgtgaa acccggcgcc gagatgctgg tcgccatcga ggaggccgag
      121 aagcaccaga aacccgtcgc attcatcgac cgggatatcc ggatcacgct cgggcggttc
      181 tggggcagaa tggggatctg ggagaagatc aaactcatcg gcgccctcat ctactcgctc
      241 gtgagcgtcg agggccagga cctcgacgtg gacgaactca cgaaccagga tgtggtgagc
      301 gctgcgatgg aagagttccg gaagttctct ccgcacggcg cccaggccct gatcgacgaa
      361 cgggacgcct accttgcgca ccagatcttg atgctcggga accggtacga tcgggtgctc
      421 gcggtcgtcg gtgccgggca catccagggg gtgcagcgct acctcaacgc gccggagacc
      481 ctgccgccgc tcccgtccct gaccgccgat gtgaagggcc tcccctgggg aaagataatc
      541 ggggcggccg tcaccatcat attcctcctc ctgatcgccg ctatagcctt ctcgggcgtg
      601 gggctcgagg tcctgatcgc cgccctcgtc tactggatcc tgatcaacgg cgtcctgagc
      661 gcggcgttca ccctgctcgc cggcggacat cccttctctg ccgccacggc gtttgccgtc
      721 tcctggttga cgtcgctcaa ccccctgctt gcggcgggat ggtttgcagc catcgtcgag
      781 gcgaagatcc gcaaacccac gacgggggaa ctccggcaga tcatcgaggc ggagactttc
      841 tccgagatgc ggcggatacc gctcttccgg gtggtgctgg tcgccgctct tgcaaacgtc
      901 gggagttcgc ttgggaccgt cgcctacttc gtcttcatct ttcccgtcct cggtatcgac
      961 ccgaccgtgg tcatcaccga gggcttctcg aacatgatcc ggatgatcgc ggggttcttc
     1021 tgagcccctc ccccggttat ccgtttcccc aaggtattta ggcctggagt gcccaccatc
     1081 gttgcatgag tccgattggt ggaacggtca acctcggcgt caccgtcata cggagccggc
     1141 acagcgtacg gaagtacaaa gagagcccga tcgaggataa ggccgtcaag gatgcccttg
     1201 actgcgcacg ccttgccccg acggcaagaa acgagcagcc ctggctcttc gggacgatcc
     1261 gggaccgcga catgctgaag gcgatcgcgg atctcgctga gaacgcccgg ttcatcgccg
     1321 atgcgcccat ctgttttgcc gtcttcggga agaaagatgc aaagtactac cttgaagatt
     1381 gctgtgcggc gacgacccag ctgatcctcg ctctccaggc ctggggggtc gggacctgct
     1441 gggttgctgg ggagaagaag gattatgccg acggtgtccg gagactcctc aacgtccccg
     1501 atgagtatgc gctcgtctcc ctcgtgcccg cgggctaccc cgaagagatc cagatcaaga
     1561 agaagaaact ccccgacgag gtcacgttct tcgagcgcta cgaggaagag aagtaggtcg
     1621 atactgcacg aactcacttt ttatcatgcc atggatgccc ggtattcact gacgtctttc
     1681 ccgggaccgg cagaatcggg acttccgggc gccggcgtca cacgtcgctc tgctttgccg
     1741 ggcagtaagg acagagggcg ttctcgacgt cgtcggcctt ctcccggacc gggcagaggt
     1801 actcgccgtt cctgatctcg accgtgagtc cgccgggaaa cggcgtgccc acggggtgag
     1861 cggggagggc gaggacgaag atgttgaagg cggcaaggag gtaatagagg aggcccaggc
     1921 gctggtcact ctcccgggtg agacatgtcc tctcgaccat cccgcagaat tcctgaaact
     1981 ctccggcgag cggctcgtcg gggaggtccg ggtcgggccg gcgcatggtg gctatgaggg
     2041 tatggtgcgt tgcaaagagc atctccatca tcagagggta gaggcgctgc cggtacccgg
     2101 cgggaacgga ctggagatcc cgctccaccc tcccccgcaa cgcctggagg tcgaagagcg
     2161 agtagcccga gacctcgcgg tagagggttg acgcgagttc tgcccgggtc gttgccgacc
     2221 ggaggcgagc gcagacccgg cggatatcca ccccgtcttc tccccggctc atcacgcgat
     2281 taatcttggt cggcagaatg catgaatcag ctcttccggg cgggtgccgc gagcgccggt
     2341 tcggggtcga ctgcccccgt gcgcccgacg ccggcgctct accggaagtg cggcaggatc
     2401 tcggccgccg cgaaatccat gaactccttc tgctgctctc cgatctggtg gatacagata
     2461 tggtcgaacc ccatccggac ccggtcctcg atctttttga tgtagggctc cgggtccggg
     2521 ccgcagggca tgccctccac cacatcttca agcgtcgcca tcttcgcgag ttgctcgtaa
     2581 tgggccggcg tggggaggag ccggttgagt tctcccgtgt ttgcggcgat gggccactcc
     2641 tcgtaggcgg tcctctctcc cacctcctcg ctctccgccc agcagaccga tatctccacg
     2701 tatgcgggtt tatcatcgcc accggatttt ttgaagacaa cgaggctcct ttcggcgttg
     2761 gtgcccccgt tgatgaaccc gtctccgacc cgcgcggcca gggaagcgct gatggggccc
     2821 tcggatgcga tggcgatcgg gggaaggctc tccgggagcg agaagacctg cgcgttctca
     2881 agcgtgtaga acgagccgta gtaggtctgc atgctccccg tccagagtgc ccggatcacc
     2941 tccaccgcct cctcaagcat ctctatcctg acgggcgccg ggggccagcg gtcgccgaat
     3001 atatgctcgt tgaggttctc gcccgatccc aggcccagca cgaaccgccc gggcatcatc
     3061 gcggcggcgg ttgcggcggc ctgggcgatg atgccggggt gtatccgtat cgtcggg
//
LOCUS       DAOSEE010000197         3081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_11237_length_3081_cov_256.422670, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000197 DAOSEE010000000
VERSION     DAOSEE010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11237_length_3081_cov_256.422670"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..669)
                     /locus_tag="PLI76_10730"
     CDS             complement(103..669)
                     /locus_tag="PLI76_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62548.1"
                     /translation="MKQIQRDGLLFELIDELTSYGSWCGETHIQKATYFLEAMMGIPL
                     GFPYVLYKHGPFSFDLSDELASMRADALLEWEIKPEPYGNALKTTSVSARLKARVPRT
                     LARYRPAISFVAERFGTRGVKDLEKISTALYVTKEAPGRGCEARARRITELKPHITLD
                     EAKEAVSFVDEMFREAEPVIRELGREET"
     gene            complement(666..2039)
                     /locus_tag="PLI76_10735"
     CDS             complement(666..2039)
                     /locus_tag="PLI76_10735"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOI62549.1"
                     /translation="MRKVNKRILFLRRQGLPEKKVRGTAKKNNHEIRDPIYAFIHINS
                     SEREVLNSAPVQRLRHIHQLALTSLVYPGATHRRFEHSLGVMELATRIYNAVLENGGI
                     DILGIDRSNYDGMYYRRVLRMAALVHDIGHAPFSHASEDLFPGDCNHEAMTLQLIESD
                     EMEKLWAMLKIQAEDVAKLALGRDHPKKKTFSNTEAILSEIITGNAFGADRMDYLLRD
                     SHHTGVAYGRFDHYRLIETLRILPKTYEGGSKEPALGVESGGLQSAEALLLARYFMFS
                     QLYLHPVRRIYDIHLKEYLQAWLEGGQFPGDVAGQLNFTDNEVMAGIWRASRDPSLPG
                     HDAAERIVMRNHYRVLYTRNPDDIMKCREVGLAIYAAACAMFGKENVRRDRPKVSASQ
                     AAFPVLEPDERIVSSLLKSDVLRHIPEVAVDTVYIRPELLDAGRVWLNENREDIIESG
                     SELEDTI"
     gene            complement(2604..2849)
                     /locus_tag="PLI76_10740"
     CDS             complement(2604..2849)
                     /locus_tag="PLI76_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsA family
                     antitoxin"
                     /protein_id="HOI62550.1"
                     /translation="MIPERCTFCKGTLHEGTAEFIARIGDEIIVIRDVPALICDRCGE
                     AYYTAEVSRKIDVVMRDVHSGRLCCRPLAAGEVELTG"
     gene            complement(2846..>3081)
                     /locus_tag="PLI76_10745"
     CDS             complement(2846..>3081)
                     /locus_tag="PLI76_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HOI62551.1"
                     /translation="FERNISTDDLFSAIKVGEIIESYPDDEPCPSLLMLGYIRDHAYH
                     VVLGICDDHLRVITAYMPDDEHWIDTRTRRKNK"
BASE COUNT          628 a          913 c          808 g          732 t
ORIGIN      
        1 gctactgggt tcgcaaagtg ggagatcctc cataccatcg ccgcccggca aagtggcgat
       61 gcatctcccc ctgaacacct atggccggca aactccggag tctcaggtct cctctctccc
      121 aagctccctg atcaccggct ccgcttcccg aaacatctcg tcaacgaagc tgaccgcctc
      181 ttttgcttcg tcaagggtga tgtgaggttt gagttcagtg atccgccgtg ccctggcctc
      241 gcacccccgg ccgggtgcct cttttgtcac gtagagggcc gtggagatct tttcgaggtc
      301 tttcaccccg cgcgtcccga atctttcggc aacaaaggat atcgcaggcc ggtacctcgc
      361 gagcgtccgg ggcacccttg cctttaacct ggcgctcacg ctcgttgttt tgagggcatt
      421 cccgtatggc tcgggcttga tctcccattc caggagggcg tcggcccgca tcgatgccag
      481 ttcgtctgag aggtcgaagg agaatgggcc gtgcttgtac agcacatagg gaaaccccag
      541 gggtatcccc atcatcgctt caaggaagta ggtggccttc tggatatgcg tctccccgca
      601 ccagctcccg taactcgtga gttcatcgat gagctcaaac agcagtccgt ctctctgtat
      661 ctgcttcata tcgtatcctc aagttctgat cccgactcga taatatcttc gcgattttcg
      721 ttcaaccata ctcttccggc atcgagcaac tctggacgga tgtacaccgt atccaccgca
      781 acttcgggaa tatgccggag tacatctgat ttcaggaggg aggatacgat cctctcatcc
      841 ggttcaagaa cggggaacgc tgcctgactc gcagatacct tcggcctatc gcggcgtaca
      901 ttctcttttc cgaacatggc acatgcggcc gcatagattg ccagccccac ctccctgcac
      961 ttcatgatat cgtccgggtt tctcgtatat aacacccggt aatggtttcg catcacgatc
     1021 ctctctgcag catcatgacc cgggagactc ggatccctgc tggctctcca tatgccggcc
     1081 atcacctcgt tatcagtgaa gttgagctgc cctgcgacat cgccagggaa ctggcccccc
     1141 tcgagccacg cctgaagata ttccttcagg tggatatcat agatccgccg cacggggtga
     1201 aggtataact gactgaacat gaagtatcgt gccagcagga gtgcctctgc cgattgaagc
     1261 cccccggact ctacaccgag ggcaggttcc tttgatccgc cttcataagt cttaggaagg
     1321 atccgaaggg tctctatcaa gcgatagtga tcgaacctgc cgtatgcgac cccggtatgg
     1381 tgtgagtccc gcaggagata gtccattcgg tcggcaccga acgcatttcc tgtgatgatc
     1441 tccgagagta ttgcttcggt attggaaaaa gtcttctttt tggggtgatc ccgacccagt
     1501 gcgagttttg ccacatcctc ggcctggatc ttcaacattg cccagagctt ctccatctcg
     1561 tcgctctcga tgagctggag ggtcatcgcc tcgtggttac aatccccggg aaagaggtct
     1621 tctgaagcat gggagaaagg agcatgaccg atgtcatgga caagagcagc cattctcagg
     1681 acacgccgat agtacatccc atcgtaatta ctacggtcga tccccaagat gtcgatcccg
     1741 ccgttctcca gcacggcgtt atagatccgt gttgccagtt ccatgacacc gagcgagtgc
     1801 tcaaaccgcc tgtgagttgc acccggatat accaggctgg tcaacgcaag ttggtggata
     1861 tggcggagac gctggaccgg cgcagagttc aggacctctc tctcgctaga atttatgtgg
     1921 atgaacgcgt agatcggatc cctgatctcg tggttgtttt tctttgccgt tcctctcacc
     1981 ttcttctcag gcaatccctg tctcctcaaa aacaaaatcc gtttattaac tttcctcaag
     2041 gtatgtgtgg agaatcgatt gggatatatc ttttgatacg cgtttggtat gctggatcgc
     2101 ccatacggcg agatatggca gacttcttct cggaagagag atcccgggat atcgggcggt
     2161 cgtatccaga attttgaaga tcaccccgcc gacgaccccg ccggcccgcg agcgctgatg
     2221 gtggaaaccc gtttctcctc cccggccttc tccgccaggg agccgctcca gtgcccggat
     2281 cctggcctca aaatcctcat cgcaccgttc catctgctac agcgagtcgc acctcacggt
     2341 gctcccgctc cggttctaac gaaccgtcgc gccttacaga ttcacttcat atcccggttc
     2401 gtcgcgtaag accgggctcc ggggtggtct ttgcaccggt catcctggta accctactgg
     2461 aggacagtag gacggtgaag cccaaaagcc tggctgtaag agttatatgc agaaaaaacg
     2521 ggcgcaacgt tgcgcatcct accgcgatgg gctggaggag aagtgtcggg tcagaaagcc
     2581 actgcgcccc ccggcctctt ccttcatccg gtgagctcaa cctcgcctgc cgcaagaggc
     2641 cggcagcaga gcctgccgct gtggacatcc ctcattacga catcgatctt ccgggagacc
     2701 tctgcggtgt aatacgcttc gccgcaacgg tcgcagatga gtgcggggac atccctgatg
     2761 acaatgatct cgtccccgat ccgtgcgatg aattcggccg ttccctcgtg cagtgtccct
     2821 ttacagaacg tgcatcgctc tggaatcatt tgtttttcct ccttgttcgg gtgtcgatcc
     2881 aatgttcgtc gtccggcata taggcggtga tgacccgaag gtggtcatca cagatcccaa
     2941 gtactacatg atatgcgtgg tcacgtatat aaccgagcat gagcagggag ggacagggct
     3001 cgtcatcggg atacgattcg atgatctcgc cgaccttgat ggctgagaag agatcatcgg
     3061 tggagatgtt gcgctcaaac a
//
LOCUS       DAOSEE010000198         2966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_11737_length_2966_cov_229.361388, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000198 DAOSEE010000000
VERSION     DAOSEE010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11737_length_2966_cov_229.361388"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(343..>2966)
                     /locus_tag="PLI76_10750"
     CDS             complement(343..>2966)
                     /locus_tag="PLI76_10750"
                     /inference="COORDINATES: protein motif:HMM:NF023252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOI62552.1"
                     /translation="AVPNYGYTGKGFDVAVSGTLHGNLTYYEFSDYTGLIHAGESREF
                     TINVPAPANGTVAYARLYIYTTWGHDEKRKEGTPVRIGLAVDGKNFIADRVYNDRKDE
                     GIYNYLLETHAFNVTGVPGGIAGEHVVKLTNDGRKDDVFATYGIGLLVVTEDPAEPEM
                     TYWIAEGSDTLYANPDFGTTSEDCITTVVFNESIDLSGTAEARVILVATAGSGMDDDE
                     HRIYFNDGEWFNLLTAGSSAISVADLDVRPFLRGSGNEGGIQSLITATKGDYMENRAL
                     ILAVWKGTPPEGYEASATSTTGTPGTPAASTGESGSRFTLNATNGRIEVTHLVADGGA
                     VQVYIPEGAVITEGAGLISALTLQRLLPGDAGGADPAYAVGNEDMKSDLPLRLVISVS
                     KSGGVIARYDRSSDTWRPLETKSDAAGSVSTTIRQGGIYAVRYGEAAPGGLLGLLFGA
                     VQALFGLFGFASTGEEPGPALAAEPTSEPRSASAEPASTVTPVPAVDIATMRFALSVL
                     SDPPGALIDLDGEYTGRTTPAAFAGLSGGAHTIRVCVDGAEPVEREIQLARDDEVVVE
                     IPGSASPGLLRTWEQNHYGGVYVDSFPEGAEIYVDGRKVNRKTPDTIYGLKEGLHTIK
                     VKKEGTVFSSDKERVWINKNALVRVMFTSGNAELTRSITFESDEHAQQPFSINGRYLG
                     DRLPKTVEIRGIGGAYLTLSDGESYRTHRIPTTAQSNDTVKAAFSDAPCSLLVESTPA
                     GAAISIDGFLTKFSTPYVVSNISEGRHLIGVSKPGHIPVEREVLLMAGSGDATVKLTL
                     KPYTWGSLEVSSNPAGAKIYLYGRDTGEVTPHTFHYLDIGSYSVKVVGKEGSKTIEDV
                     LVSPYTTTECHAEITGG"
BASE COUNT          513 a          967 c          915 g          571 t
ORIGIN      
        1 ggtgcaggag accacccggt cgcccgtata cctgaaaaaa cgtcctcaaa tattccggag
       61 ccgggagccc ccgaatcgga aaaagcgtgc agtacggctg cgccgggaaa agtgacggct
      121 cgtcccggca caggaggatg gccctgtaaa ccttaacatg gagatggttg aagaagaaca
      181 gggccgtctt tacttttcgt actcatgcat gattatcctg ccggaacgtc gtgctgcaaa
      241 cacacccccg gaaacgatcg cacacagctg catggctggc cgtgcggcca caccccgcgc
      301 agaggccagc taccgcccaa aaaacagggc gatggggacc tctcagcccc ccgtaatctc
      361 ggcatggcat tcggtggtcg tatacggcga gaccagcacg tcctcgatcg tcttcgaccc
      421 ctccttcccc accaccttca ccgagtaact gccgatatcg aggtagtgga aggtatgggg
      481 cgtcacttcg ccggtatcgc ggccatagag gtatatcttg gcgcccgccg ggttgcttga
      541 gacctcaagc gacccccagg tatagggttt gagggtgagc ttcaccgtgg catcgccgga
      601 accggccatt agcagaacct cccgctcaac cggtatgtgt cccggctttg agacccctat
      661 caggtgtctc ccttccgata tgttgctgac gacatacggc gtggagaact tcgtcaggaa
      721 cccgtcgatc gatatcgccg ctcccgccgg cgtcgactcc acgagaaggc tgcagggcgc
      781 atcggagaac gcagccttca cggtatcgtt cgattgggcg gtggtgggta tcctgtgcgt
      841 ccggtaactc tcgccgtcag agagggtgag atatgccccc ccgatccccc tgatctcgac
      901 cgtcttcggg agccggtcgc ccaggtacct gccgttgatc gagaacggtt gctgcgcgtg
      961 ctcgtccgac tcgaacgtga tcgagcgcgt caactccgcg ttgccgctgg taaacatcac
     1021 ccgcaccagg gcgtttttat taatccagac ccgttctttg tcggaactga acacggtccc
     1081 ctctttcttc accttgatgg tgtgcagccc ctccttcagc ccgtagatgg tgtcgggggt
     1141 cttccggttc accttgcggc cgtcgacgta gatctctgcc ccctccggga acgagtcgac
     1201 gtagacgccg ccgtagtggt tctgctccca ggtgcggagc aggccggggg aagcggaacc
     1261 cgggatctcg accacgacct cgtcgtcgcg cgcaagctgg atctcccgct ccaccggctc
     1321 ggcgccgtcc acgcacaccc ggatcgtatg ggcgccgccg gagagccccg cgaaggcggc
     1381 cggggtcgtc ctgccggtgt actccccgtc gaggtcgatg agcgcgcccg gcggatcgga
     1441 gagcaccgag agagcaaacc gcatcgtggc gatatcgacg gcaggtaccg gtgtaaccgt
     1501 agatgcgggc tctgccgagg ccgatctggg ttcagacgtg ggctccgccg ccagcgccgg
     1561 tcccggctcc tccccggtgg aggcgaagcc gaagagcccg aagagcgcct ggacggcacc
     1621 gaacagcagg ccgagcagcc cgcccggggc cgcctcgccg taccgaaccg cgtagatgcc
     1681 gccctgccgg atcgttgtcg agacgctgcc cgccgcatcg ctcttcgtct ccagcggccg
     1741 ccaggtgtcg gaagagcgat cgtacctggc gatcacgcct cccgacttgc tcacggagat
     1801 gaccagcctg agcgggaggt cgctcttcat atcctcgtta ccgaccgcgt aggccgggtc
     1861 ggcgcccccc gcatcgccgg gtaaaagcct ctgcaacgtc agcgccgaga tgaggccggc
     1921 accttcggtg atcaccgcac cctcggggat gtacacctgg acggccccgc cgtcggcgac
     1981 gaggtgcgtg acctcgatcc gcccgttcgt cgcgttgagg gtgaaccgcg agccggactc
     2041 accggtcgat gccgccggag tcccgggcgt gcctgtcgtg gacgtggcgc ttgcttcgta
     2101 gccctccggt ggggtgccct tccagacggc gaggatgagg gcgcggttct ccatgtaatc
     2161 tcccttcgtc gcggtgatga ggctctggat ccctccctcg ttccccgacc cgcggaggaa
     2221 cggtctcacg tccagatcgg ccacgctgat tgccgaagag cctgcggtga gcaggttgaa
     2281 ccactcgccg tcgttgaagt agatccggtg ttcgtcgtcg tccatccccg aaccggccgt
     2341 ggcgaccagg atgacgcgcg cctcagcagt cccggagagg tcaatagact cgttgaaaac
     2401 caccgtcgtg atacagtctt cagacgttgt cccgaagtcc gggttggcat agagcgtatc
     2461 cgacccctcc gcgatccagt aggtcatctc cggttcggcg gggtcttcgg tgacgaccag
     2521 gagcccgatg ccgtaggttg caaagacatc gtccttcctc ccgtcgttcg tcagtttcac
     2581 gacgtgctcg ccggcaatgc cgcccggaac gccggtgacg ttgaacgcgt gcgtctcaag
     2641 cagatagttg tagatcccct cgtcttttcg gtcgttatag accctgtcgg ctatgaaatt
     2701 ctttccgtcg accgcaaggc cgatcctgac cggggtgccc tctttcctct tctcgtcgtg
     2761 cccccaggtg gtatagatgt agagccgtgc atatgcgacc gtgccgtttg caggagcggg
     2821 gacgttgatc gtgaattccc tgctctcccc ggcatggatg agcccggtgt aatcgctgaa
     2881 ctcgtagtag gtcaggttcc cgtggagggt gcctgagacg gcgacatcga acccctttcc
     2941 ggtgtagccg tagttgggaa ctgcgc
//
LOCUS       DAOSEE010000199         2955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_11797_length_2955_cov_243.105862, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000199 DAOSEE010000000
VERSION     DAOSEE010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11797_length_2955_cov_243.105862"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..420)
                     /locus_tag="PLI76_10755"
     CDS             complement(1..420)
                     /locus_tag="PLI76_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62553.1"
                     /translation="MDLNFCRHYAGDGTPPSNRYCRVCPEAACGRLWQRVLDLAASNG
                     GDPVPLPGTRAVLFPNEKNPDFVRLQVNCRWGLPKEDFLHYIATGHAGMGRRGQRSDP
                     GASPSCTRQEPYVQAIVTLLGGMDTPEIRAVREVQRG"
     gene            complement(428..688)
                     /locus_tag="PLI76_10760"
     CDS             complement(428..688)
                     /locus_tag="PLI76_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOI62554.1"
                     /translation="MTYTVTYTARARRNLKRLPPRAAIRIIQAVAGLRNDPYAQVRKL
                     AGFTRTPVYRLRVGEYRVVLVVEDERLLVLILGAGHRSIIYR"
     gene            complement(685..921)
                     /locus_tag="PLI76_10765"
     CDS             complement(685..921)
                     /locus_tag="PLI76_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62555.1"
                     /translation="MSSTTVWVTPENKSRLAALKRHPKESYNDVIARLIDMAVDDEPL
                     SEEAIRGIEEALEDIRAGRLYTEDEIEREFGAKE"
     gene            1428..2249
                     /locus_tag="PLI76_10770"
     CDS             1428..2249
                     /locus_tag="PLI76_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62556.1"
                     /translation="MGVCIAAIYAVGMLLVAAACAGGYPGIADALDTGMVETQAEQSA
                     ARAGTVTLPHTGPTENTYSNGTTVVYFPAGWVSGLRNATQNADIGFDSGYAGSSGETK
                     YLNNLTAALDPYDHIRLREGYKLEGYRVSDYLQAYIVPFVVEENVTALPMTNRTRLLP
                     EGADKNISRYIEGDDSPESYLEASVFLRELYAIGAWWHARIGWPQQTVLESPEMPGVA
                     INSTHATVTIHTEELYCGYRTVTRYTDTYSRMNYEVSAAERTTVSQEADPRWPCY"
     gene            complement(2600..>2955)
                     /locus_tag="PLI76_10775"
     CDS             complement(2600..>2955)
                     /locus_tag="PLI76_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOI62557.1"
                     /translation="DWNGWIAFFLHAIEEQAGTNGQKAKAILDLYDEMKRTVPEATRS
                     QYAIAAIDALFGAPIFSSAEFYERSAIPKVSGAKILKDLEGNDVVEILQRGAGRRAST
                     YAFPRLLAITEGNRL"
BASE COUNT          572 a          865 c          888 g          630 t
ORIGIN      
        1 tcaccctctc tgcacttccc gcaccgcccg tatctccggt gtatccatcc cgccgagcag
       61 cgtaacgatc gcctgcacgt agggctcctg ccgggtgcag ctcggggacg ccccggggtc
      121 gctccgctgc ccccgccggc ccatccccgc gtgaccggtg gcgatgtagt gcaggaagtc
      181 ctccttcggg aggccccacc ggcagttgac ctgtaatcgg acgaagtcgg ggttcttttc
      241 gttcgggaag aggaccgccc gcgtcccggg gagcgggacc gggtctccgc cattcgatgc
      301 ggcaagatcg aggacccgct gccagagccg gccgcaggcg gcctcggggc agacccggca
      361 gtagcggttc gacggcggcg tgccgtcgcc ggcgtagtgg cggcagaagt tcaggtccat
      421 ggtatcttta ccggtagatg atgctccgat gcccggcccc caggatgagg accaggaggc
      481 gctcgtcctc gacgacgagc accacccggt actcgcccac cctgagccgg taaaccggtg
      541 tcctggtgaa cccggccagt ttccggacct gcgcatatgg gtcatttcgg agtccggcaa
      601 cggcctggat aatccggatg gccgccctgg gggggagcct tttgagattc cgccgggccc
      661 gggcggtgta tgtgaccgtg taggtcattc ctttgcaccg aattctctct cgatctcatc
      721 ttcggtgtag agccggccag ctcggatatc ctcgagggct tcctcgatac cccggatggc
      781 ctcctctgat aagggctcat cgtcgacggc catatcgatc aggcgggcaa tgacgtcgtt
      841 gtaggattct tttggatggc gcttcagggc cgcgaggcgg ctcttgttct cgggggtgac
      901 ccagactgta gtggaggaca tgttaatgcc tataggttgt tgcaggtctt gatacatgtg
      961 gtcgagcagg catgcccctt cccattccag cacatgccat cccgcatccc gggaacaggg
     1021 gcatgcatgg atatcacagt cttgtcggat cttgtcctgc tgcagtcctg cgttgtacaa
     1081 actcctggca gtggccgcgc gagcgtatcg atgatccgat agttcttcct ggatatgcag
     1141 gcaattgctg acgatccgcg gtgctattga gaagatactc tgtcgctcat cgatcaagaa
     1201 gagccgtgcg gtgtgagagt gtaaaggcaa ttttccctcg ctggcgcagg ggatctcctt
     1261 accccgagag gtgaagggag gcggcaccct ctctcctgat atgggcctgc aacgtgccgt
     1321 tgtatcccta tccctttccc ggcacaggac tccgtgcatg gcagtaaagg ctaaatcttc
     1381 tgaatcacaa gggcagccgg tcaagggatg tggggaatat gcgaagagtg ggggtctgca
     1441 tcgctgctat ttacgccgtc ggaatgcttc tcgttgcagc ggcgtgtgca ggtgggtatc
     1501 cgggcattgc cgatgcactg gatacaggca tggttgagac tcaggcagag caatcggccg
     1561 caagagcagg caccgttacg cttccgcaca ccggccccac ggaaaatact tatagcaacg
     1621 gcactacggt cgtgtatttc cctgcgggct gggttagtgg ccttcgaaat gcgacacaaa
     1681 atgcagacat cgggtttgac tcaggctacg ccgggagtag cggcgagaca aagtacctaa
     1741 ataatctcac tgccgctctc gatccctatg atcatatcag actgcgagaa ggatacaaac
     1801 tcgaaggata ccgggtatcc gactatctgc aggcgtatat cgtcccgttt gtcgtagaag
     1861 aaaacgtaac cgctctgccg atgacaaaca ggacccggct cctgccggag ggagcagata
     1921 agaatatctc gcggtatatc gaaggagatg actcgcccga gtcgtacctc gaagcatcgg
     1981 tgtttctcag ggaactgtat gcgatcggtg cgtggtggca tgcaaggatt ggatggcctc
     2041 agcagaccgt tctcgaatcc ccggagatgc cgggtgtcgc cataaactcg acgcatgcga
     2101 cggtgacgat acacacggaa gagctctact gcggctaccg gacggtaacc cggtataccg
     2161 atacgtactc gaggatgaat tacgaagttt cggcagcaga aaggacgacg gtatcgcagg
     2221 aagcggatcc aaggtggcct tgctattgag tgtgccgaac cggggcccgc actttgtctc
     2281 cccatcgaag gccccaggaa acctgatgag gaatgcacgc cgggccgcct tatctctgcg
     2341 gagcccgcgg ccttcccgct tcgccccgat ggtccggcgg tcggccgggt ccgatcgttg
     2401 tgcgtcgccg gcgtcgcata aaattggttg tgagacacca aagggcgctt catggcccgc
     2461 aaaatgtgtt gtggctcata ggtgtcccgc aaccgcctcc gaggtatggt ttgtgcatac
     2521 cggagcatat acaagatctg ttgtgcatat caaatccagg ttttgatata cacaaatggg
     2581 tttgttggta ccagaatact cacaaccggt tcccctcggt gatggcgagc aggcgcggga
     2641 aggcatacgt gctcgccctt cgccccgccc ccctctgcaa tatctctacg acgtcgtttc
     2701 cctccagatc cttgaggatc tttgccccac ttaccttcgg gattgccgac cgttcgtaaa
     2761 attcggccga gctgaatatc ggcgccccga agagcgcatc tatcgcggcg atggcgtact
     2821 gcgaccgggt cgcttccggc accgtccgct tcatctcgtc atagagatcg aggatcgcct
     2881 ttgctttctg gccgttcgtt cccgcctgct cctcgatggc atggaggaag aacgctatcc
     2941 agccgttcca gtccc
//
LOCUS       DAOSEE010000200         2906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_12072_length_2906_cov_232.454227, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000200 DAOSEE010000000
VERSION     DAOSEE010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12072_length_2906_cov_232.454227"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="PLI76_10780"
     CDS             <1..1074
                     /locus_tag="PLI76_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOI62558.1"
                     /translation="MIRETGTCKGIENYSRHFDGRKAGEQPYCLLDYFPEDFLMVIDE
                     SHQTLPQVRGMYHGDYSRKKSLVDYGFRLPSAFDNRPLKFDEFSGYMRNVIFVSATPG
                     DYELGRSSVAEQIIRPTGLVDPAVEVRPIEGQIPDVIAEIRATIDRGDRILLTTLTKR
                     LAEELSEYLAEQGIKTRYLHSEINTIERTEIIRQLRLGKYDVLVGINLLREGLDIPEV
                     GFVGILDADKEGFLRDARSLVQTIGRAARNVNAKVVLYADTMTDSIRKAITETERRRT
                     MQLAYNARHGITPQTIRKPIREREVDIADIKHVPKSEIPNLIIELEADMREAAERLEF
                     ERAIALRDTIKKLQEGGAGEIRR"
     gene            complement(1090..1311)
                     /locus_tag="PLI76_10785"
     CDS             complement(1090..1311)
                     /locus_tag="PLI76_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOI62559.1"
                     /translation="MKRLYRSRTDRWIAGICGGIGEYLEIDPNVIRVIWAIGTAVTGF
                     VPGIVVYILLWIILPESGMARPVEVPVGA"
     gene            complement(1397..2653)
                     /locus_tag="PLI76_10790"
     CDS             complement(1397..2653)
                     /locus_tag="PLI76_10790"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HOI62560.1"
                     /translation="MDRLHLILQRYFGHATFNPYQREIIQDLLAGRDVLAVLATGGGK
                     SLCYQVPAVMGNGVTLVISPLIALMKDQVDDLQARGIGAEALNSSGSYRTTCRILSEL
                     EENLIQILYVSPEKAVSDDFLALVASLPVTLIAIDEAHCISMWGHQFRPEYRSLAVLK
                     ERLSGVPTVALTATATPDVRDDIARQLDLADPAVYVGSFNRENLRYAVIAKGEDPYAE
                     LRTCLQDRKAGAGIVYTATRDGAETLSARLQADGMAALPYHAGMTAAARARTQDRFLH
                     GKAGVVCATSAFGMGIDKPDVRFVVHYDMPDTLEAYYQESGRAGRDGATGDCILFYHD
                     DDAKRLRSFIDRDLPSEFRRDVARSKLQSMVEYCTATGCRRERLLAYFGEDFNAPCGG
                     CDACAPQTGKVRQGRRKRGAVRSASG"
BASE COUNT          545 a          938 c          928 g          495 t
ORIGIN      
        1 atgatccgcg agaccgggac ctgcaagggg atcgagaact actcccgcca cttcgacggg
       61 aggaaggccg gcgaacagcc ctactgcctg ctggactact tccccgagga tttcctgatg
      121 gtgatcgacg agagccacca gaccctgccc caggtccgcg gcatgtacca cggcgactac
      181 tcgcggaaga agtcgctcgt ggattacggg tttcggctcc cgtcggcgtt cgacaaccgg
      241 ccgctcaagt tcgacgagtt ctcggggtac atgcgaaacg tcatattcgt ctcggcaacc
      301 ccgggcgact acgaactcgg gcgttcgagc gtcgcggagc agatcatccg cccgaccggg
      361 ctcgtggacc cggcggtcga ggtccggccg attgaagggc agatcccgga cgtgatcgcc
      421 gagatccggg ccacgatcga tcgcggcgac cgcatactgc tgacgaccct taccaaacgg
      481 ctcgccgagg aactctccga gtacctggcc gagcagggta tcaagacccg ctacctccac
      541 tccgagatca acaccatcga gcgaaccgag atcatccggc agctccgtct cgggaagtac
      601 gatgtcctcg tggggatcaa cctcctccgc gagggtctcg atatacccga ggtcgggttt
      661 gtcgggatcc tcgatgccga taaagaaggg ttcctccgcg acgcaagaag cctggtccag
      721 accatcgggc gggccgcccg gaacgtgaac gcaaaggtcg tgctctacgc tgacaccatg
      781 accgactcga tcaggaaggc cataaccgag accgagcggc ggcgcacgat gcagcttgcc
      841 tacaacgccc ggcacggcat cacgccgcag acgatccgaa aaccaatccg ggagagagag
      901 gtcgatatcg ccgatatcaa gcacgtcccg aagtcggaga taccgaacct gatcatcgag
      961 ctcgaggccg atatgcggga ggcggccgaa cgcctggagt tcgagcgggc gatcgcgctt
     1021 cgtgatacga tcaagaagtt gcaggagggg ggtgcgggag agatccggcg gtgaggccgg
     1081 ccctcccctt cacgctccga cgggcacctc gaccgggcgc gccatcccgc tctccggcag
     1141 aatgatccag agcaggatgt agacgacgat cccgggtaca aaacccgtga ccgcggtgcc
     1201 gatagcccag atcacccgga tgacgttcgg atcgatctca aggtattccc cgatgccgcc
     1261 gcatatgccg gcgatccacc ggtcggttct cgagcggtat aacctcttca tggcaactcc
     1321 gcagggtcat tttaccgtat gcgtaatagg tgtttccccc gggagttcgg gagaaagggt
     1381 gccggaggcc ggcacctcat ccggacgcgg aacgaaccgc accgcgcttc ctgcgcccct
     1441 gccggacctt cccggtctga ggggcacagg cgtcgcaacc gccgcaggga gcattgaagt
     1501 cctccccgaa gtaggcgagg agcctctccc tccggcaccc ggtcgcggtg cagtactcca
     1561 ccatgctctg cagtttcgag cgtgcgacgt cgcgccggaa ctcggacggg aggtcgcggt
     1621 cgatgaagga ccggaggcgt tttgcgtcgt cgtcgtgata gaagaggatg cagtcgcccg
     1681 tcgctccgtc cctccccgcc cggccgctct cctggtagta ggcctcgagc gtatccggca
     1741 tatcgtagtg gacgacgaac ctgacgtccg gcttatcgat gcccatgccg aacgcactcg
     1801 tcgcgcagac gaccccggcc tttccgtgca aaaaccggtc ctgtgtcctg gcccgggcgg
     1861 ccgccgtcat gccggcgtgg tagggcaacg ctgccatgcc gtcggcctgg agacgggccg
     1921 agagcgtctc ggccccatcc ctcgtcgcgg tatagacgat accggcgccg gcctttcggt
     1981 cctgcaggca ggtgcggagc tctgcatacg ggtcttctcc cttggcgatg acggcatacc
     2041 tgaggttctc ccggttgaag ctcccgacgt agaccgccgg gtctgcgaga tcgagttgcc
     2101 gcgcgatatc gtcgcggacg tccggcgtcg cggtcgccgt cagggcgacc gtcggcacgc
     2161 cggagagacg ctccttgagg accgcaagcg accggtactc cggccggaac tggtgtcccc
     2221 acatcgatat gcagtgcgcc tcgtcgatcg ctatcagcgt gacggggagg gatgcgacga
     2281 gagcgaggaa gtcgtcgctg accgccttct ccggcgagac gtagaggatc tggatcaggt
     2341 tctcttcgag ttcggagagt atccggcatg tcgttctgta ggacccggaa gagttcagcg
     2401 cttcggcgcc gatcccgcgt gcctgcaggt cgtcgacctg gtccttcatc agggcgatga
     2461 gaggcgatat cacgagcgta acgccgttgc ccatgaccgc agggacctgg tagcagagcg
     2521 atttgccccc tccggtcgca agcaccgcaa ggacgtcgcg cccggcaagg aggtcctgga
     2581 taatctcccg ctggtatggg ttgaacgtcg catgcccgaa gtagcgctgc aggatcaggt
     2641 gcaggcgatc catcactctc tattggcgct ccggcggcat agtatcttcg gccgcaaaaa
     2701 tcctgcagaa tgcagccgga ttcctgccgg agctcctaac ccgagacctc gtcccagaac
     2761 ccggcgacgg cggagaatat ccgctcccgg aggtcggcga gccgtgcctc gtcgaccggg
     2821 tgctccgcgg tctcgtagag gccgcggttg atctcgatct ggacccaggg aatacgggtc
     2881 cgccggtagt gcgccaccga tatgaa
//
LOCUS       DAOSEE010000201         2893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_12142_length_2893_cov_229.253700, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000201 DAOSEE010000000
VERSION     DAOSEE010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12142_length_2893_cov_229.253700"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PLI76_10795"
     CDS             <1..546
                     /locus_tag="PLI76_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62561.1"
                     /translation="PTVTPGVPAKSMKLELYKGWNFVSIPRPLSPGNNTAIEVFRDVE
                     TGGRPIYTYTPENGFEPLGADTTLKVLDGYWVYSTEAMTVQLNFSTNPVMVPASKMLS
                     PGWNAIGYSDLTPRSANESLISVEDSWVSVVGYNAKNQNYQPALINGQTGAHGENQKL
                     LPTKGYWLFMREDGTLAAINA"
     gene            complement(835..1110)
                     /locus_tag="PLI76_10800"
     CDS             complement(835..1110)
                     /locus_tag="PLI76_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAC family transcription factor"
                     /protein_id="HOI62562.1"
                     /translation="MTNEKEGDYCTVCGGIKPEAIKIKSVLVDGKATGINQLDFIVAG
                     VRDLHLADDAAIRAELLRRAGEFNYIPTKKKEAYADALLQEYKEVTR"
     gene            complement(1107..1454)
                     /locus_tag="PLI76_10805"
     CDS             complement(1107..1454)
                     /locus_tag="PLI76_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOI62563.1"
                     /translation="MAKEKRAELQGKVLRLPELVAYQDGTVASRMIVNNRSGSITLFS
                     FDEDEGLSEHTAPYDAVVTILDGECEVWIAGETHQMSAGETIIFPADVPHALSAITRF
                     KMSLTMIRGREDT"
     gene            complement(1508..2281)
                     /locus_tag="PLI76_10810"
     CDS             complement(1508..2281)
                     /locus_tag="PLI76_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOI62564.1"
                     /translation="MVIESIPKVVGFIYGLLAFIALVGLWYSGRFTRRRALPFLVISA
                     LLGFLVFAPVFPYQLQMVALRDTVGLGSPIPMALGALLVFIALAFAFGRVICGQVCPA
                     GAVQELMYRVPVAKRGSAERQTPVVIRAGVFVVFAVAALGLSADLLGLIGLPAFFHLA
                     VGSVSFFVFLGIVLLSAVVYRPFCRYVCPYGALLAPAASKALFRLRRTDACIGCRKCE
                     RACPTGEADPAAGLGECYLCGRCTDVCPVEGALVYGRAR"
     gene            complement(2289..2627)
                     /locus_tag="PLI76_10815"
     CDS             complement(2289..2627)
                     /locus_tag="PLI76_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOI62565.1"
                     /translation="MKIVDVAKEPISKTPHNVDARKVYDTEHATAVVITLAPGESLKK
                     HITPVDVFFYVLEGTGVVEIGDERAEVGKDHLVESPAKIPHLWANESKAPFKVLVVKV
                     PRPTTGTRLL"
BASE COUNT          578 a          923 c          850 g          542 t
ORIGIN      
        1 ccgacggtga cgccgggagt gccggcaaag agcatgaagc tcgagctgta caaaggctgg
       61 aacttcgtct ccatcccaag gccgctctct ccaggtaaca acaccgcaat tgaggtattc
      121 agggatgtcg agaccggtgg aaggccgatc tacacataca ccccggagaa cggctttgag
      181 cccctgggcg cggatacgac cctgaaggtc cttgatggct actgggtcta ctcgaccgag
      241 gccatgaccg tgcagctgaa cttcagcacc aacccggtga tggttcccgc atccaagatg
      301 ctctccccgg gatggaatgc catcggctac tccgacctga caccgcggag cgcaaacgaa
      361 tcgctcatat cagtggaaga cagctgggtc tctgttgtcg gttacaacgc aaagaaccag
      421 aattaccagc cggcgctcat caacggtcag acaggagcac atggtgaaaa ccagaagctc
      481 ctcccgacca agggctactg gctgttcatg cgcgaggatg gcacactggc tgccatcaac
      541 gcctgaccct ttttttgatg cttcccggga ccatttcatc ctgaaacccg cgctttccaa
      601 ccgaacgccc ggtctgtttt acaaaaaaga gcgtgaaccc gtgcacctca taccgggtac
      661 ccgcaccgcc atccgcatgc cggcacctcg cacgttgcca tgaaggccgc actggagagc
      721 ggctcaccgc caacccgatc gttgtgctct tttcggcggt gcaagcgccg gcagcggggg
      781 tcaaaggaga gaaccccgcg cgaaggttca cgcgggggag cgtactccgg cgcctcaccg
      841 ggtaacttcc ttatattcct gcaacagggc gtcggcgtac gcctctttct tcttcgtcgg
      901 tatgtagttg aactccccgg ccctccggag gagttcagcc cggatcgcgg catcatccgc
      961 aaggtgcagg tcccgcacgc cggcgacgat gaagtcgagc tggttgatgc cggtcgcctt
     1021 cccatccacg aggaccgact tgatcttgat cgcctccggc ttgatcccgc cgcagacagt
     1081 acaataatcg ccttctttct cgttggtcat gtgtcctctc tcccccggat catcgtgagc
     1141 gacatcttga accgtgtgat ggcagagagg gcgtgcggga catctgccgg gaatatgatc
     1201 gtctcgcccg cgctcatctg gtgcgtctcg cctgcgatcc agacctcgca ctcgccgtcg
     1261 aggatagtca ccaccgcatc gtagggcgcg gtgtgctccg agagcccttc gtcctcgtcg
     1321 aatgagaaga gggtgatgct tcccgaccgg ttgttgacga tcatcctgct cgccacggtc
     1381 ccgtcctggt aggcgacgag ttccggtaac cggagaacct ttccctgtaa ctctgcacgt
     1441 ttctcttttg ccactggtac accagcctct cttctttatt ctggcgtcct tcgcttattt
     1501 acccttctca tctcgcccgc ccatacacga gcgccccttc caccgggcag acatccgtac
     1561 accggccgca gaggtagcat tccccgagac cggcggcagg gtccgcctcc ccggtcgggc
     1621 atgcccgctc acacttccgg caccctatgc aggcatccgt ccgcctgagc cggaagagcg
     1681 cctttgatgc cgcgggtgcg aggagggcgc cgtatgggca gacgtagcga cagaacggcc
     1741 ggtagacgac ggcggagagg aggacgatgc cgaggaagac gaagaacgac acgctcccga
     1801 ccgcgaggtg gaagaaggca ggtagaccga taagcccgag gaggtctgcc gagagaccga
     1861 gagcggcgac ggcgaagacg acgaagacgc cggcccggat caccaccggc gtctgcctct
     1921 cggcgctacc gcgctttgct accggcacac gatacatgag ttcctggaca gcccccgccg
     1981 ggcagacctg cccgcagatg acccggccga aggcgaaggc aagggcgatg aagacaagga
     2041 gcgccccgag cgccataggg atgggtgacc cgaggcccac cgtatcccgg agggcgacca
     2101 tctgcagctg gtaggggaag accggcgcga ataccaggaa cccgagaagc gccgatatga
     2161 ccagaaacgg cagcgcacgc cgccgggtga accggccgga ataccagagc ccgacgaggg
     2221 cgataaaagc aaggaggccg tagatgaacc ctacgacctt cggaatcgac tcgatgacca
     2281 ttacatcctc agagcagcct cgtcccggtc gtcgggcgcg ggaccttcac cacgagcacc
     2341 ttgaaggggg ctttgctctc gtttgcccag aggtgcggta tctttgccgg actctcgacc
     2401 aggtggtcct tcccgacctc tgcgcgctca tcgccgatct cgacgacccc cgtcccctca
     2461 aggacgtaga agaagacgtc caccggggtt atatgcttct tcaacgactc tcccggcgca
     2521 agggtgatca ccaccgcggt cgcatgctcg gtgtcgtaga ccttccgggc gtcgacgttg
     2581 tgcggggttt tgctgatcgg ttcttttgct acatcaacga tcttcataac actcatctcc
     2641 aatgttatac gggactctcg tgatatttat ctttgctgag ttgaaggggc ggagcgggaa
     2701 gaccccctgc acaagcgagg ggtagttcac cctttatagt ttgtccagta gttttcgagg
     2761 cactcctgcc gcctccgctt ctgccggtgg gcccgcatct cgggataggt cggttcaacg
     2821 ctctggaccg ccgggcaata ggggcagagg gcgaaagcca cgtcgtcggc cttatccctg
     2881 accgggcaga ggt
//
LOCUS       DAOSEE010000202         2831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_12470_length_2831_cov_228.519452, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000202 DAOSEE010000000
VERSION     DAOSEE010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12470_length_2831_cov_228.519452"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PLI76_10820"
     CDS             <1..258
                     /locus_tag="PLI76_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOI62566.1"
                     /translation="VGNIQWPRPVHLLPRFRHAAGRQRDVFPLTGYLTGRIINIPIHC
                     GVTSEDIEGMAACLREFSSGAGSGVADRLRVTGVEAATHGV"
     gene            248..1231
                     /locus_tag="PLI76_10825"
     CDS             248..1231
                     /locus_tag="PLI76_10825"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOI62567.1"
                     /translation="MVFDYTDSVSPGVWRSFLNGGEGIYFFHTPEWAKVMEGAFGYRV
                     ATRLYHTPEGDVLVPMMESRRFGFSVYQSVPHGYGGIFSPSSLSAGVLTDLVGSMVGG
                     RHLLLDLALPPGVALQRERSPGVTKTLSEWNYTHILRLDDPGSAVEQRYNRHARRDIG
                     IAERNHVEIVRDDSLAQFQRYYALYEQRAREWGYPEPPYPRKVYDLLYRYGRPYVRVR
                     LAEMQGEVIGGLVTFEYADTVFAWSNAAPAASRPFCPVHLLLHDALRSAQEEGYSLVN
                     FGGSGNLLGVRKFKESFGAVQVDLETYRITSRLASVMRKGWWPAYLRKSSG"
     gene            complement(1248..2732)
                     /locus_tag="PLI76_10830"
     CDS             complement(1248..2732)
                     /locus_tag="PLI76_10830"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HOI62568.1"
                     /translation="MKESTRFAFDISWVFVSQIVTLGSGFLLNIVLSRWLGPADFGLY
                     TLTLTIFTISTLIAGLGIPETVVKYVADYRSDQERLNAVVTAGVINALVLGVAAGLAL
                     FFFAGDIAGSFGMPELSGLIRIIAVAVPLAIVNTTLLGLLNGLREMRSYSFRFMIRSL
                     LLITLAVLFIAAGLGPGGAVLALLLTEVGTLAFVVSIVRRHFRLSVPGYPETSRKMLA
                     FGSRVFIASAVYLINTYTDTLLIGYMLTEIDVGRYAIAIAIARVAFLSLPGSISTVNY
                     PAISEYYSKGLHDAVEAVITRSIRYCLILLSTMGLALIYLAETIIGFLFGPAYLPAVP
                     AFIILVFGMIVFGAVSSVGPAVSAMNRPGLSSKVNIFVAGTNVCLDVALIPLFGITGA
                     AIGTAGACTLLAVLILYVLHHVFGVRVESAMIIKAFGVVSVFFIAFLLLRGTVHPYVL
                     TVVFSGLYGIYAYRFLLTAEDKTEIAGMIAPVFGRIAARRLNGR"
BASE COUNT          560 a          807 c          946 g          518 t
ORIGIN      
        1 gtggggaaca tccagtggcc gaggccggtg cacctgctgc cccggttccg gcacgccgcg
       61 gggcggcaga gggatgtgtt ccccctgacc gggtacctga ccggacggat catcaacatt
      121 cccatccact gcggcgtgac atcggaggat atcgagggca tggctgcctg ccttcgcgag
      181 ttcagcagcg gcgccgggtc cggcgtggcg gatcgcctga gggttaccgg agttgaggca
      241 gcgacgcatg gtgtttgatt acaccgattc cgtctctccg ggcgtctgga gatcgtttct
      301 caacggcggg gaggggatct acttcttcca tacgcccgag tgggcgaagg tcatggaagg
      361 ggcgttcggc taccgggtgg cgacaaggct ctaccatacc cctgaaggcg atgtcctggt
      421 cccgatgatg gagtcacggc ggttcgggtt ctccgtctac cagtccgtcc ctcacggcta
      481 cggcggcatc ttctcgcctt cgagcctctc ggccggggtt cttacggatc ttgtcgggag
      541 catggtcggg gggaggcacc tcctcctcga tctcgccctg ccgccgggtg tcgcattgca
      601 gagagagaga tcccccggcg tcacgaagac gctctccgag tggaactata ctcatatcct
      661 gcgcctggac gatccgggaa gcgcggttga gcagaggtat aacaggcatg cgcgccggga
      721 tatcggcatt gccgagcgaa accacgtcga gatcgtcaga gatgatagcc tcgcgcagtt
      781 tcagcgctac tacgccctct atgagcaaag agcgcgggag tggggctacc cggaaccgcc
      841 ctatccccgg aaggtctacg acctcctcta ccgctacggc cgcccgtatg tcagggtgcg
      901 cctggcggag atgcaaggcg aggtgatcgg gggtctcgtg acgttcgagt atgcggatac
      961 ggtctttgca tggagcaatg cggccccggc ggcgtcccgc cctttctgtc cggtgcatct
     1021 ccttctccac gatgccctgc ggtctgcaca ggaggagggt tactcgcttg taaacttcgg
     1081 gggaagtgga aaccttctcg gggttcggaa gtttaaagag agtttcgggg ccgtgcaggt
     1141 cgatctcgag acctaccgga tcacgtcccg tctggcgtcg gtcatgcgga agggctggtg
     1201 gccggcgtat cttcgcaaat cctccgggta gcccggggcg tgcctgctca ccgcccattg
     1261 agacgccgtg ccgcgatgcg cccgaatacc ggggcgatca tgccggcgat ctccgtttta
     1321 tcctccgctg tcagcaggaa ccggtaggca tagatgccgt acaggccaga gaagaccacc
     1381 gtgagcacgt aggggtgaac tgtaccccgc agcaggagga aggcgatgaa aaatacgctg
     1441 acgaccccga acgcttttat gatcatcgcg gactcgaccc gtacaccaaa gacgtggtgg
     1501 agcacgtaga gtatcagcac cgcgagcagc gtacacgctc cggccgtgcc gatagccgca
     1561 ccggttatgc cgaagagggg gatgagcgcc acgtcgaggc agacgttcgt ccccgccacg
     1621 aagatgttca ccttcgagga gagaccgggt ctgttcatgg cgctgacggc cggcccgacg
     1681 gaagacaccg ccccgaagac gatcatgccg aagacgagta tgatgaacgc aggcacggcc
     1741 ggcaggtagg cggggccgaa gaggaacccg atgatcgtct cggcgagata gatgagggcg
     1801 aggcccatcg tcgagaggag gatcaggcaa taccggatcg acctggttat cacggcttcg
     1861 accgcatcgt ggagtccttt gctgtaatac tccgatatcg cagggtaatt gaccgtggag
     1921 atcgaaccgg ggagcgagag gaaggccacc cgggctatcg cgatcgcgat cgcataccgg
     1981 ccgacgtcga tctccgtcag catgtatccg atgaggagcg tatcggtgta ggtgttgatc
     2041 aggtagaccg cgcttgcgat gaagacccgg ctcccgaacg cgagcatctt cctcgacgtc
     2101 tcagggtagc cggggaccga gaggcggaag tgccggcgga cgatcgagac gacgaaggcg
     2161 agtgtcccga cctcggtgag caacagcgcc agaacggctc ctccgggtcc gaggcccgcg
     2221 gctatgaaca gcaccgccag ggtgatcagg aggagcgagc ggatcatgaa ccggaacgag
     2281 taggatctca tctcccgcag gccgttgagg aggccaagga gcgttgtgtt gacgattgca
     2341 agcggcacgg caacggcgat gatccggatg aggccggaga gttccggcat cccgaaagat
     2401 cctgcaatgt ctcccgcaaa gaagaagaga gcaagaccgg cagcgacccc gagcacgagc
     2461 gcgttgatga ctcctgcggt gacgacggca ttcagccgct cctggtcgct cctgtagtcg
     2521 gcgacgtact tcacgaccgt ctcgggtatg ccgaggcctg cgatcagcgt ggatatggta
     2581 aagatggtca gcgtcagggt gtacagtccg aagtccgccg ggccgagcca gcggctcaac
     2641 acgatgttta agagaaaccc cgagccgagg gtgacgatct ggctgacaaa tacccagctg
     2701 atgtcgaatg cgaacctggt agattctttc atgggttgtc gggtccggtc aatggcggta
     2761 tctggtatac tgccggtatt tatagtgttc ttttgattcg ggatgatacg gggggatcat
     2821 cgagtgcggg g
//
LOCUS       DAOSEE010000203         2790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_12714_length_2790_cov_231.763437, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000203 DAOSEE010000000
VERSION     DAOSEE010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12714_length_2790_cov_231.763437"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="PLI76_10835"
     CDS             complement(<1..744)
                     /locus_tag="PLI76_10835"
                     /inference="COORDINATES: protein motif:HMM:NF020698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasin domain 3-containing protein"
                     /protein_id="HOI62569.1"
                     /translation="MKLQTYAYLAVGLLLLAAPAGALIPDNITISASPVWLTAGGDNA
                     ATITVQVKNSTSPLEGVGVDFDVDGTYGSISPLHAVTDSDGKAMADFRPGTVAGTAKI
                     TATVPYEGPDEPLKESIDLQIDHTIPHAIAGLWYKPEVTAGGEVDITVRMEDRYGNVV
                     DSRREDALGISLQAENVTFMVGSPGGGAAFAGDRDKIPVPVDENGNATARLRVDTVAG
                     ENVVYIQPPKPIKGEYISITGTADGMPAGI"
     gene            complement(995..2392)
                     /locus_tag="PLI76_10840"
     CDS             complement(995..2392)
                     /locus_tag="PLI76_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOI62570.1"
                     /translation="MVKRRLIIALLVSCLLLCCTAQAVTLRISVADEKTGSALVDASI
                     YVDGDYIGSTASDGTYSYVHSGKKDLRLKVVRSGYRDWLDYVGADRTGIVVNMARKDE
                     ILTFELYDAGTLRPVVGALVRIEGDGVSSSGTSGSGGSTTFTVRAGALYNVEVRASGY
                     YDLSKTVQMESSDRLVQYWLFSSDLLAVQVRDGATSVPIEGAEVFVDGSRAGATDVDG
                     RLPLHLQREKRYSLRITAPDYQPYQEERYLGAGDVFLPVHLSKSAYPVSVTAFCEAMK
                     PVEGAEVSLNGTLQGKTNQYGRLALSDIHAGTYELLVRAPGYEDWSVTRQISGKGEDI
                     VADLKYDRASVTVRVEGPDRKAVAGAVIIIDGRTAGVTDSLGCFKTALTTNRVYAVAA
                     ARDGYQNVSVDADIPLGTAEFTVPLVMEQAVNVWVLVAGIGVVAAVILGAIFMVRRRQ
                     AGQSRSRSPRGRDGL"
     gene            complement(2432..>2790)
                     /locus_tag="PLI76_10845"
     CDS             complement(2432..>2790)
                     /locus_tag="PLI76_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HOI62571.1"
                     /translation="KMRQIIEIQGGDPTVKADDIVPGDYRFVVRAPQDGYVIELNNSA
                     LVTLARLAGSPYDHGAGLEIHAKKGVRVRKGDPIFTIYADREWRLERTIEVGRTLMPV
                     LVEGMVLERVPPERWV"
BASE COUNT          526 a          924 c          802 g          538 t
ORIGIN      
        1 gatacccgcc ggcataccgt ccgccgtgcc ggtgatggag atatactcgc ccttgatagg
       61 tttcggcggc tggatgtaga cgacgttctc cccggccacc gtatcgaccc ggagccttgc
      121 ggtggcattc ccgttctcat ccaccggcac cgggatctta tcccgatcgc cggcaaacgc
      181 cgccccgccc ccgggcgaac ccaccatgaa ggtcacgttc tccgcctgga gagaaattcc
      241 cagggcatcc tcccgccggc tatccacgac attcccgtac cggtcctcca tccggacagt
      301 gatatccacc tcccccccgg cggtcacttc cggcttatac cagaggccgg cgatcgcatg
      361 cggtatcgtg tggtcgatct gcaggtcgat gctctccttc aacggctcat ccggcccctc
      421 atacggcacc gtcgcggtga tcttcgcggt tccggcaacg gtcccgggcc tgaagtcggc
      481 catcgccttc ccatcggaat cggtcaccgc gtgcagggga gatatgctcc catacgtacc
      541 atccacatcg aagtcgacac cgactccctc gagcggggag gtgctgttct tgacctgcac
      601 cgtgatggtt gcggcattgt cgccccccgc cgtcaaccac acggggctcg cgcttatcgt
      661 gatgttatca ggtattaacg ccccggccgg ggccgcaaga aggagcagcc cgacggcaag
      721 gtacgcatat gtctgtagtt tcatacttca ccgtatctgc tcctatcaca acaaagtata
      781 taaatattac taatatgata aacaagccag aaggctctgt ttgcgaatag ggcaatgata
      841 ttcacgctgt ttgaatattt gttcgatgaa aggaccccat gtgagaaaag agatattact
      901 ttatcctgta tctgaaatta ttttttcttc cggggataca tcctgtcgcc ggaccccggg
      961 aaaaaaagta cttccgggga ggggccgggg agacctataa cccgtcccgg ccccgtggcg
     1021 accggctccg actctgcccg gcctgccggc gccgcaccat gaagatcgca ccgagaatga
     1081 cggccgcaac gaccccgatg ccggccacaa gcacccagac gttgacggcc tgctccatca
     1141 cgagcggaac cgtgaactcg gcggtaccga gcggtatatc agcatcgacc gagacgttct
     1201 ggtaaccgtc gcgggcggcg gcgacggcat acaccctgtt cgtcgtgagc gccgtcttga
     1261 aacacccgag gctgtcggtc accccggcgg tccgaccgtc gatgatgatg accgcaccgg
     1321 cgaccgcctt ccggtcgggc ccctccacgc ggacggtcac gctcgcccgg tcatacttca
     1381 gatcggcgac gatgtcctcc cctttcccgg atatctggcg tgtaacactc cagtcctcgt
     1441 agccgggcgc ccgaaccagg agttcatacg tgcccgcgtg gatatcggag agcgccaacc
     1501 ggccatactg gttggtcttc ccctggagcg tcccgttgag ggagacctcc gcaccctcga
     1561 ccggtttcat cgcctcgcaa aaggccgtta ccgagaccgg gtatgccgac ttcgagaggt
     1621 gcaccgggag gaagacatcg cccgcgccga ggtaacgctc ctcctggtag ggctggtaat
     1681 ccggtgccgt aatcctgaga gaataccgct tctcccgctg caggtggagt ggcagtctgc
     1741 cgtcgacatc ggtcgctccg gcgcgcgaac cgtcgacgaa gacctccgca ccctcgatgg
     1801 ggacggatgt cgcgccgtcc cgcacctgga ccgcgagaag gtcgcttgag aagagccagt
     1861 actggacgag cctatcgctg ctctccatct gcacggtctt tgagagatcg tagtagcctg
     1921 acgcgcggac ctcgacgttg tagagcgcac ccgccctcac cgtgaaggtc gtgctcccgc
     1981 cgctcccgct cgtccccgag ctcgataccc cgtcgccctc gatacgcacc agagccccga
     2041 cgaccggtcg cagggttccc gcatcgtaga gctcgaacgt gaggatctcg tctttgcggg
     2101 ccatgttgac tacaatgccg gtcctatcgg caccgacgta atccagccag tcccggtagc
     2161 ccgaccgcac caccttcaac cgcaggtctt tcttcccgga atggacgtag gagtaggtgc
     2221 catccgatgc agtgctcccg atgtagtcgc cgtcgacgta gatggaggcg tcgacgagcg
     2281 cgctaccggt cttctcgtcg gccacgctga tccgaagggt gacggcctgc gccgtgcagc
     2341 agaggaggag gcaggagaca agaagggcta tgatgagacg acgttttacc atgctggagt
     2401 gtactgcgcc caaaaatttg agtgcatcgg tttacaccca gcgctcgggc ggaacacgct
     2461 caaggaccat cccctcgacg aggaccggca tcagcgtccg gccgacctcg attgtgcgtt
     2521 caagccgcca ttcacggtct gcgtagatgg tgaagatggg atcgcccttc ctgacccgga
     2581 cccctttctt tgcgtggatc tcgagccccg cgccgtggtc gtagggcgaa cccgcaagcc
     2641 gggcaagcgt gacgagcgcg ctgttgttga gctcgatgac gtagccgtcc tgcggcgccc
     2701 ggacaacgaa cctgtaatcg cccggaacga tatcatcggc cttcaccgtc gggtcgccgc
     2761 cctggatctc gatgatctgc cgcatcttct
//
LOCUS       DAOSEE010000204         2423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_15198_length_2423_cov_251.117399, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000204 DAOSEE010000000
VERSION     DAOSEE010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15198_length_2423_cov_251.117399"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PLI76_10850"
     CDS             <1..472
                     /locus_tag="PLI76_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62572.1"
                     /translation="LPALRGRETPTVWQLQAATGLPYRKTRRLLICSRLQEKCPAVSG
                     VDSTVIGDSAGTTVRRHELHFRFDAGRCRERAAELAIRLEDDPDDRGNDPHVYTFAPG
                     LHRNSGERANEDTGWSGGDSSIGEICTDQNGNLHHLPGTESLRAGASDPHSRSL"
     gene            748..966
                     /locus_tag="PLI76_10855"
     CDS             748..966
                     /locus_tag="PLI76_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62573.1"
                     /translation="MTPAVTIDDLYLEIKKIQETMVRREDLDALLDTVEILSNPETMA
                     QIRMSEEDIAARRYREISSIDDLLGELS"
     gene            966..1238
                     /locus_tag="PLI76_10860"
     CDS             966..1238
                     /locus_tag="PLI76_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62574.1"
                     /translation="MEWTIRRTDTFVESLSKVKGNKEVIIELGKKLQRLKQDPLNTGG
                     WLSGALHGKKATRIAKRYRLIFLPDERERAVYLIAIDHRQHVYRLR"
     gene            complement(1541..1921)
                     /locus_tag="PLI76_10865"
     CDS             complement(1541..1921)
                     /locus_tag="PLI76_10865"
                     /inference="COORDINATES: protein motif:HMM:NF019407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin N-terminal domain-containing
                     protein"
                     /protein_id="HOI62575.1"
                     /translation="MACRDRDDGVSEVVSTILMVAMAVILAAIAGSMVFGMELPEEPK
                     TVVVTATRSGDVITFMNHGGMNMDRVAEIKCWIGGVGQGNDNFTIDARAGATETHTSK
                     EAVRVVVVGRFVDSESWIVLDKTL"
     gene            2191..>2423
                     /locus_tag="PLI76_10870"
     CDS             2191..>2423
                     /locus_tag="PLI76_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI62576.1"
                     /translation="MGGGGRGGGTLILSPFFLGRISLSIRPCRIIVVLTERASPRLLA
                     YAVPVLMLFSLAWLAVVDFRFATSCWEGYHFWIP"
BASE COUNT          552 a          756 c          631 g          484 t
ORIGIN      
        1 tcttcccgct ctgagggggc gggagacgcc cacggtctgg caactccagg cggcaacggg
       61 actcccctac cggaagaccc ggcggctcct catctgctct cgcctccagg agaagtgccc
      121 tgccgtcagt ggggtggact cgacggtcat cggcgactcc gccgggacga ccgtccgccg
      181 gcatgaactc cacttccggt tcgacgccgg gcgctgccgg gagcgggcgg cagaactcgc
      241 gatccggctg gaggacgacc ccgacgatag gggcaacgac ccgcatgttt acacctttgc
      301 accaggtttg caccggaatt ccggggaacg tgcaaacgag gatactggct ggagtggcgg
      361 tgattcttct atcggagaga tatgtacaga tcagaatggt aatttacacc atttaccggg
      421 aacggagagt ctgcgtgcag gagcgagtga cccccactcc cggtctctgt gaaatccgca
      481 gtggtgcaaa catactaact attcgcgagg agaccctccc gatccgggaa cagcctgcaa
      541 cctgcagttt ttcgaggtgt aaaccaaggt gcaaaggggt gtaaaccggt gtaactgtgc
      601 aaacgaatac cccggtcaac cccgaatccc caggttgctc cgcgcaagac cacgtcgccg
      661 tccccgcaac cgttccttcc cgcgaaatga gaagggcgcc caccacctcc agaatacttt
      721 tatttgaaca gacggttagt actactcatg acaccggctg tcaccatcga tgacctctat
      781 cttgaaatca aaaagataca ggagactatg gtcaggcggg aagatcttga cgcccttctt
      841 gataccgttg agattctcag caatcccgaa actatggcgc agatacgaat gagcgaagaa
      901 gacatcgcag ccagacgata tcgggagata tcttcaatag acgacctgct cggcgagctg
      961 tcctgatgga atggacgatc cggaggaccg atacatttgt cgagagcctc tcaaaggtca
     1021 aaggcaacaa agaagtaata atcgaactcg gaaaaaaact tcaacgcctg aaacaggacc
     1081 ccctcaatac gggcggttgg ctttcgggtg cattacacgg gaagaaagca acccggattg
     1141 caaagcgtta tcgactgata tttcttcccg acgaaagaga aagagcagtg tacctgatag
     1201 caatcgatca tcgtcagcac gtctacaggt tgcggtgaaa gcctgccgca tcacggatat
     1261 gctccatata gtcatcaggc gtcctgcccc gggtaagata tccggtaatg ctctccgaaa
     1321 catccagtga cgaaaacacc ctcactgcat gagtttttgc tatgggaaca ggttatcgct
     1381 caacgtgatc cggtcagatt cagttctact ctggagcact gccttgctcc cctctcctgt
     1441 ccccttcgcg ccaacgcgag agcccccgtc tccgtcctca caaccgttgc ctcgcacacg
     1501 aaaaaaaggc cctgcccgca gataatggcc gtccggtgca tcagagcgtc ttatccagca
     1561 ctatccacga ctcgctatcg acaaacctcc cgacgacgac aaccctgacc gcctccttcg
     1621 aggtatgcgt ctcagtcgcc ccggcccggg catcgatcgt gaagttatcg ttcccctggc
     1681 ccaccccgcc gatccagcac ttgatctcag ctacccggtc catattcatc ccgccgtggt
     1741 tcatgaacgt gatcacatcc cccgaccgcg tcgccgtcac cacgacagtc ttcggctcct
     1801 caggaagttc catcccgaag accatcgacc ccgcaatcgc cgcaaggatg acagccatgg
     1861 ccaccatgag gatcgtgctc accacctccg agacaccgtc gtcccggtcc ctacacgcca
     1921 tatacgggat aatctcgggt ctctgtataa taggatgccc atgtcgtgct ggccgggaaa
     1981 aatacttccc cgaggctata acggcgggaa atattattgt aggaaccgcc cctccccggc
     2041 gccggaagaa ttcgttagcc gcccggcctg aacaccctct atatgcatgc tccgctctgc
     2101 aacgggcccg ttgtgccgtc gcggtctagg tcctgaaggt ggaggaggac gaggacgacg
     2161 aaccggagta tgagaaaaaa atgaagacga ttgggaggag gcggaagggg aggagggaca
     2221 ctcattctct cccctttttt cctcggtagg atctcccttt ctattcgccc ttgccgtatc
     2281 atcgtcgtcc tcacagaacg tgcctccccc cgcctcctcg cctacgcggt cccggtgctg
     2341 atgctcttct cgctcgcctg gctcgcggtc gtcgacttcc ggttcgccac cagctgctgg
     2401 gagggctacc acttctggat acc
//
LOCUS       DAOSEE010000205         1883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1813
            NODE_20955_length_1883_cov_215.406455, whole genome shotgun
            sequence.
ACCESSION   DAOSEE010000205 DAOSEE010000000
VERSION     DAOSEE010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863342
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:02:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,177
            CDSs (total)                      :: 2,147
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 30
            tRNAs                             :: 30
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20955_length_1883_cov_215.406455"
                     /isolate="AMR_MDS_1813"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..529
                     /locus_tag="PLI76_10875"
     CDS             128..529
                     /locus_tag="PLI76_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOI62577.1"
                     /translation="MQPDNEQLIDDFLAHADDLGDDIVADVKEMLGLVPFIFTILRDR
                     PDVFALSTIADYRTSRPDSLDSKTAELIAVAAAASAGADSCLKVHIGAALKEGASRDE
                     VLDTLLIAAMIGKTRILASSLRQFREVCGGK"
     gene            611..1186
                     /locus_tag="PLI76_10880"
     CDS             611..1186
                     /locus_tag="PLI76_10880"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOI62578.1"
                     /translation="MSQTKAALRAQAREARSLLSREEAAIQSATIERHLLDLLDGFET
                     VMVYTAKPQEVDTSGLIADLNRRGVRVVVPIIERETVSLRLSYLPDPAVLVPSTFNVP
                     EPIGHELPARPGDIEAVVIPMLAFDIEGNRLGYGAGYYDRFLCRYPRPQRIGIAFSCQ
                     RAESIPADENDVKMDCIVTETGIRRYNRRGP"
     gene            1247..>1883
                     /locus_tag="PLI76_10885"
     CDS             1247..>1883
                     /locus_tag="PLI76_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOI62579.1"
                     /translation="MANTESVEVTIKEAAHEDAGRGIARLSIDTMKVLGLVSGDVVEV
                     EGRQKAATLIWPGFPQDTGKAILRIDGSTRSNVGAGIDDKVRIRKTEAGYAKKVTIQP
                     TQLIRLVGGEQYLGRLLRGRPVTEGQHVRVNILGNPLTFVIAKVAPKGIAIVTENTEI
                     ELKETPYEPKEGKREVAGDIHYEDIGGLDRELQLVREMIELPLRHPELFERL"
BASE COUNT          399 a          623 c          556 g          305 t
ORIGIN      
        1 atctgcccgc ttacgctgcc gccgggaagg agcgtacacc actgccggcc ggactctcgc
       61 gaacttccgt ggccttcgcg cgagtttatc gtcggcgccg tccaggatca tagaagagga
      121 cagttccatg caacccgata acgagcaact gatcgacgat tttctcgctc acgccgacga
      181 cctcggcgac gatatcgtgg cggatgtcaa agagatgctc ggcctcgtgc cgttcatctt
      241 caccattctc cgtgaccggc ccgatgtctt cgcgctctcc accatcgccg attaccggac
      301 ctcccgcccg gattctctcg attcaaagac cgcggagctc atcgccgttg cagctgcggc
      361 gtctgccggc gccgacagct gcctcaaagt ccacataggg gccgccctga aagaaggcgc
      421 atcccgcgac gaggtcctcg acaccctcct gatcgccgcg atgatcggaa agaccaggat
      481 cctcgcatcc tccctgcgcc agttcaggga ggtctgcggc gggaagtaga cgccgggggc
      541 gtagcaggcg cccgcacccg tgccggctca ggcacccttt tatggtctga ttgagccaaa
      601 acttcctgca atgagccaga caaaggcggc cctccgggca caggccagag aggcccgctc
      661 ccttctctcc cgcgaggaag ccgccatcca gagtgcaacg atagagcggc acctcctcga
      721 cctcctcgat gggtttgaga ccgtcatggt ttacaccgca aaaccccagg aggtcgatac
      781 ttccggcctt atcgccgact taaaccgccg gggcgtacgg gtcgtcgtcc ccatcatcga
      841 gcgggagacc gtgagcctcc gcctctccta tctgccggac cccgcggtcc tcgtcccgag
      901 cacgtttaac gtgcccgaac cgatcggcca cgagctcccc gcccggccgg gggacatcga
      961 ggccgtcgtc ataccgatgc tcgccttcga catcgaaggg aaccggctcg gctacggtgc
     1021 cggctattac gaccgcttcc tctgccgcta tccgcgcccg cagaggatcg gcatcgcgtt
     1081 ctcctgtcag agggcggaga gcatccccgc cgacgagaac gacgtgaaga tggactgcat
     1141 cgtcacagaa acggggatac gtcggtacaa caggagggga ccatagaaaa atataaatat
     1201 ttgtttacac ttacctatgg taaacataaa ggaggagatt gttcatatgg ccaacaccga
     1261 atccgttgaa gtgaccatca aggaggcggc tcacgaagac gccggccggg ggatcgccag
     1321 actgagcatc gacaccatga aggtgctcgg cctcgtcagc ggcgacgtcg tcgaggtcga
     1381 ggggcgccag aaggcggcga cactcatatg gccgggcttc ccgcaggata ccggcaaggc
     1441 gatcctgcgc atcgacggca gcacccggag caacgtcgga gccgggatcg acgacaaggt
     1501 ccggatcagg aagaccgaag ccggatacgc aaagaaagtg accatccagc cgacccagct
     1561 catccgcctc gtgggcggcg agcagtacct ggggaggctc ctccgcggcc ggccggtcac
     1621 ggaagggcag cacgtccggg tcaacatact cggaaacccg ctcacgttcg tcatcgctaa
     1681 agtggcaccg aaaggcatcg ccatcgtcac ggagaacacc gagatcgagc tcaaagaaac
     1741 cccgtacgag cccaaagagg ggaagcgcga ggtggcgggc gatatccact acgaagatat
     1801 cggcggcctg gaccgcgaac tgcagcttgt ccgcgagatg atcgagctcc cgctccggca
     1861 ccccgaactc ttcgagcgcc tcg
//
