LOCUS       DAOSEW010000001        26011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_1481_length_26011_cov_5.096355, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000001 DAOSEW010000000
VERSION     DAOSEW010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 26011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26011
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1481_length_26011_cov_5.096355"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PLJ02_00005"
     CDS             <1..561
                     /locus_tag="PLJ02_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOI04844.1"
                     /translation="IILAEMLDMQLAQNEGIPPLEQIMEMNLYKTARYTFSLPLMLAG
                     HLADQSPAMINNLSEIGEKVGLIFQMRDDYLGIFGDNKKTGKPILSDIREGKKTIFYY
                     YLIKSELLSPKDKQLLNSCLAVEQISQENSLTLQKLFKNHSLPAVNQQMKKLEDETLG
                     IIDNLNNDKLAKLLKEILNFAQYRQC"
     gene            complement(571..1803)
                     /locus_tag="PLJ02_00010"
     CDS             complement(571..1803)
                     /locus_tag="PLJ02_00010"
                     /inference="COORDINATES: protein motif:HMM:NF017761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04845.1"
                     /translation="MKLPKLAFQKNKNLKHLYAIRLMRELVNKLVMFFLPIYYFNLHL
                     PFWDKINWSLSLNGQNTLTALQIGVFNIALFQLLDRLVAFLSAIWVAEWTIKLGTKHA
                     FVMGQILYVLFAAFLFLSQINPYWIFLAVIIDGVQTNYFWNSYYYSLSRNSEHHKIGS
                     NLGIINFLLNIITMISPALGGLIIAGLGYGNLFLLGLVLITIGLIFSISMDNVKVHDK
                     ISWEEFSTWMMEPGFRRLGLTFAGSYFNDGVLQMWPLYIFLLLGTSDKVGFLYSFSLL
                     LAMVISYGVGAVIDKHKSRRPFVLSAGILSLFWIIRSFVVGFWSIVVVNTLDRITSSF
                     HWLFFDRVWVVRGKGREGLSYFTYRQMITSAAAIVFWSLVFFLFYFFGEAWISLFLVA
                     AVGVLLTLLVKEHQDPKV"
     gene            complement(1825..2358)
                     /gene="pth"
                     /locus_tag="PLJ02_00015"
     CDS             complement(1825..2358)
                     /gene="pth"
                     /locus_tag="PLJ02_00015"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOI04846.1"
                     /translation="MKIYVGLGNPGPEYQGTRHNAGFMFVEAMAEALSLTFKFDKKFN
                     AQLAVKRDDLFLIKPQTFMNNSGQALRAFLDYLKIDLQDPFAGPEDLIVVHDDLDLPL
                     GTYKLQKGTGPKEHNGLQSIYQHLGTKDFWHLRLGIDARNGLRNIAPADYVLQRMPPE
                     QQAIFDQSVKQLLQELL"
     gene            2415..3536
                     /locus_tag="PLJ02_00020"
     CDS             2415..3536
                     /locus_tag="PLJ02_00020"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOI04847.1"
                     /translation="MKKNIGIFGSNQGHQSIAEAIKEKIESQAGDRYQVHILYTKQPL
                     SLIYNSIYRFAPKIFDRPYEFSANMVENYAELNDLVSSFFLKSTYQLAKDFIEEKNID
                     LTISTYFSFQPTLEKIQQEKQIPYFNVIANPRTIHPLEISPQAKINFFFDDKNLTEYQ
                     KRCRAKVTGWFVGKRFETTYQKEAVRKKLKITPALTFLIASGSEGSTAVLKILPALIN
                     CPKPLQVFVACGNNKNLYSNVLGIQKSLVAFSQSQAIITPLKFTKNLHLYMQAADLII
                     GKAGPNTLFESVATITPFFAITHLAQEEGNLAIIKEHNLGIVAEQSQAANQKISAIIR
                     QPKQLAKFKPGLLNIKQYNQQSASILIEEIDTILQKSVK"
     gene            complement(3561..5381)
                     /locus_tag="PLJ02_00025"
     CDS             complement(3561..5381)
                     /locus_tag="PLJ02_00025"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HOI04848.1"
                     /translation="MIFDQFAQYLADLEKISSRLEMTELLAQLFKKLGKDEIVPASYL
                     IQGSLVANYRSLEFQLSNKMLIRSLASFYARHAKPDVDHQSLFTQGLFDQDDQQTIVS
                     ELTKKYKNLGDLGELFYQVVSDLATVDGQEEALTIVEVHQQLELIAGEKGEGSQDRKI
                     NLLADLLDQVSALSAKFIARIILGKMRLGFSTMTMLDALSWAKTGDKSQSALLEEAFQ
                     KKADVGLLAQLYLFDENLSAKELAKNYQLELGIPVVPALCQRLNSGQEIIDKMGEVIA
                     EPKYDGLRIQIHINKNGFADGSFYKAYTRNLEEVSAMFPELEQVLTQCQAQTAIFDSE
                     AIAYDPQTKKFLPFQETISRKRKYQVDVKAEETPICFYVFDLLFYNGRALIDEPLIDR
                     KKLLDEAIQDSTVVKKTIYQLINDPQTLSDFHQKQLAVGLEGAVMKKPESAYVAGRKG
                     WRWVKIKEAEGQRGKLNDTLDCIMLGYYRGKGKRQSFGIGALLVGVLDQDTGKIKTLS
                     KIGTGLTDDQFRAIKQLADQYRSPQNQANPQYDFKKELKADVWIEPNLVLEIAADEIS
                     QSPSHTAGVALRFPRLVTIRQDKNWQQATTLQELKEIRQA"
     gene            complement(5460..6572)
                     /locus_tag="PLJ02_00030"
     CDS             complement(5460..6572)
                     /locus_tag="PLJ02_00030"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOI04849.1"
                     /translation="MSLMYQPHYRISNNLLTYISAIESAKAVIDNSPLVPAWEAKFRD
                     EALTRSVHYGTKIEGNDLSADQASQVLSLGKTDDVQTVLDQTGIIARERDIQEVINYR
                     NVLAWIDQQPLLKKNTDQQSSLLNLSTLNLLHSLTMDKLLDEEQIATLRNKQVIVRGA
                     KNGQVVFRPPVAAEVPFLLDDLFAWINSEESQNLHPILRAAIIHYQLVYIHPYVEGNG
                     RTARAFATLALYEAGYDFKRFFSLEEYFDSDVDAYYQALLSVQQHPKQDLTYWLEYFA
                     YGLALEIDKVKAKVAKLSQDLKLKRELGQQVALSERQIILLELLQNQGTMTSDDAQKV
                     LPNVSVDTILRDLKDLIAKGVVKKHGVTKGVSYSLS"
     gene            6719..7702
                     /locus_tag="PLJ02_00035"
     CDS             6719..7702
                     /locus_tag="PLJ02_00035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOI04850.1"
                     /translation="MIENPQQSVQAQQLIDQAFHNLKIAKKDSHKGQNGKLLIIGGSK
                     LFHAASKWSLEVASKIVDMVFYSSVPSNNRLIKKWISAELDHQLTQAVKENFWQGIVI
                     DRRQIEDYLDEADCILLGPGMERTNYTAKLSNKLLKKFPQKKWVIDAGALQMVDPHLL
                     SQNCIITPHQQEMDILVQKNQGFAPNNYQALPICLLKGPVDRILISKNHSLSTKTTLI
                     EITGGNAGMTKGGTGDVLAGLLAALYCQNDALTSTIIASYINKKAGDSLYKSVGPYFN
                     ASDLVAKIPQVLWETLHSRESLKNQENQVEYLHSFGDSLIILARKRSGKTL"
     gene            7705..8256
                     /locus_tag="PLJ02_00040"
     CDS             7705..8256
                     /locus_tag="PLJ02_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04851.1"
                     /translation="MTKEETVDHNPVSHPDLGDSREGSNNSENGDYDDDGHGNDGYAN
                     NDHKVSSEQNKTATIAENPYSIPPIFNKYKNMSLDTFFKILDAKSKKPQEIDDDLDVV
                     LTFLRDLENSIKIAQQLTDAHGNPCFPALYEQHCSRAEYLSKLLEQFRWFTELKKNPR
                     FDISTIGHNGYNNSKKDNNKTVF"
     gene            complement(8253..9437)
                     /locus_tag="PLJ02_00045"
     CDS             complement(8253..9437)
                     /locus_tag="PLJ02_00045"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOI04852.1"
                     /translation="MLLRKLALQNFRNFTNQNWDFGRTTIFVGPNGSGKTSIVEAINL
                     LATGDSFRATHVEEMIRLEAELSRVQGLFSLSNEEDLFAEKDWSGENDWSEKKDSLAE
                     KDWLGEKKEATADDLKLEVMLTRGMVAGRRSQYRLFTVNDVRRRRKDFVKNLSVVVFR
                     PEDLRLIEGSPSRRRLFLDQALSMLDWEYQRALQQYEQTLKRRNLLLQKVREGEESKG
                     ILSFWNTNLLEHGKFLQRERSHFVDFIREVIFPFELNIEYLPSLINEERLKKYADQEI
                     VVGHTLIGPHKDDFLVKFSLPEKGIEDLSLLAYGSRGQRRLGVLWLKIAELQFLHSRI
                     DYQPLLLLDDIMSELDESSKAMVLSLLENYQVVLTTADGDLATALSKKIQQSQLIELK
                     SV"
     gene            complement(9480..9788)
                     /locus_tag="PLJ02_00050"
     CDS             complement(9480..9788)
                     /locus_tag="PLJ02_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04853.1"
                     /translation="MDKLAIVESLIYSLGQIVLGLFLHPYQSMQNLVRDKVFVPLMFL
                     PTLLATICYLLFAWWLLDLFYDGSIVFRLIYRSFFFFFLYWQILLIYLFWRFKRAFHN
                     "
     gene            complement(9781..9984)
                     /locus_tag="PLJ02_00055"
     CDS             complement(9781..9984)
                     /locus_tag="PLJ02_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04854.1"
                     /translation="MPDKQEFLKQLKTEAKIISSLKKEKFIPDRFSTLANFVATHSWQ
                     IILFLALISSLVWELFLYQGNNG"
     gene            complement(10015..10563)
                     /locus_tag="PLJ02_00060"
     CDS             complement(10015..10563)
                     /locus_tag="PLJ02_00060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOI04855.1"
                     /translation="MQRFLGFSNEQVLVEAIKNGHESAIEYWFKNYHKQLLNLAMQKL
                     PNRSIAEEIVQETFINCLRSITLFKGDSSLLTYMQSILRHEINDFYRKRYAKKFIKTI
                     PLSEFLLDHEYKDAHETAILVKQVLKKMGSQSQELLMKKYVDKKRVKELAQEFGRSEK
                     AIESELFRARKEFRLLWANLEK"
     gene            10613..11029
                     /locus_tag="PLJ02_00065"
     CDS             10613..11029
                     /locus_tag="PLJ02_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04856.1"
                     /translation="MKKTITLFSLVLFIFVAYLFLTQKGILTPNVIYAETENVEIDLS
                     EHLTLGNGQRLAEQFATPASMINLLIRVVFVGVGLILLAMIIISGLSMIAAGTTESKD
                     KASTTMTSALMGFLVIFAAYWILQIIQIFTGVNLGF"
     gene            11059..11658
                     /locus_tag="PLJ02_00070"
     CDS             11059..11658
                     /locus_tag="PLJ02_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04857.1"
                     /translation="MIKIKTIKLLLPLLFFLLFLRPVKAQTIIPCGTPGIKKCTTTET
                     GQPAYAVCAADGITWKRYACTAGRVCLPSDPSCVDPSEHPEAVVGAIDAPPGVAKFNA
                     QADSGLGIILFFSNLIKLIAIVAGLWTMINFIIAGFTYVTSADNPSAIEKIGSKLSSS
                     VIGLAIIVASYTIAAVIGLILFGDAGFIISPEIPTVALP"
     gene            11680..12063
                     /locus_tag="PLJ02_00075"
     CDS             11680..12063
                     /locus_tag="PLJ02_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04858.1"
                     /translation="MSNIHTLIAQNSDQSIFGTINAPQGVAEFNQQAGNIGIILFFSN
                     LIKLIAIVAGLWTMINFIIAGFTYVTSADNPSAIEKIGSKLSSSVIGLAIIVASYTIA
                     AVIGLILFGDAGFIISPEIPTVALP"
     gene            12085..12480
                     /locus_tag="PLJ02_00080"
     CDS             12085..12480
                     /locus_tag="PLJ02_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04859.1"
                     /translation="MSNIHTLIAQNSVNIGSLKPVTEARQPENLETVTVLSTLELWIS
                     DIIGVLTILGTLFFIVYAFISAFNIITASGDKGKLEKAQNRLVWSTLGLILMVATYAI
                     IGLIGSIIGLELLQPGAMLQTIIPTGIQP"
     gene            12513..12935
                     /locus_tag="PLJ02_00085"
     CDS             12513..12935
                     /locus_tag="PLJ02_00085"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04860.1"
                     /translation="MKKNLVYLNLAVVVTLLFFLLTPSVLAQTQGITNPVIGDLGTND
                     PSVSSGGRFIQYTVYLWRASITLGSLAVIAFFLLGAFEWITSGGDKTKVENARNKITS
                     AVIGLVLLVSSFVLLSFLSKLLFAGEFDLLKLTVPGIN"
     gene            12969..13310
                     /locus_tag="PLJ02_00090"
     CDS             12969..13310
                     /locus_tag="PLJ02_00090"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04861.1"
                     /translation="MHQQLAQVTLDIAGEAQKQGLLGAEQAQTADGGFGNWLSNILTV
                     VMVLGAVVCLALIIMGAIQWITSGGDKAKTEEARNRITSAIIGLIVLAATIALFSLVA
                     DFLGITAIKFI"
     gene            13381..13785
                     /locus_tag="PLJ02_00095"
     CDS             13381..13785
                     /locus_tag="PLJ02_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04862.1"
                     /translation="MKSIKKFALASASALSGLLVPIKMAMAAFDDIEINPGTGYATDF
                     GNMFSSILNVVMLIAAVLVFAFLIFGGIQWITAGGDKSKAEEARNRITSAIIGLVIVA
                     ASYALINLIVNFLGFGSFNDVFKNVGTINDAN"
     gene            13853..14236
                     /locus_tag="PLJ02_00100"
     CDS             13853..14236
                     /locus_tag="PLJ02_00100"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04863.1"
                     /translation="MNKNFALLPLVKPVQAQTESWEDINPNCVVDGTATIQGIGCLLA
                     NVLSVILTVIGLTGFVMIIYAAFIMLTSAGKSQQVEKSKNTITMAIVGIIIALSSFII
                     INLIANFTGISIFKTLTIPGSSTTW"
     gene            complement(14250..14960)
                     /gene="uppS"
                     /locus_tag="PLJ02_00105"
     CDS             complement(14250..14960)
                     /gene="uppS"
                     /locus_tag="PLJ02_00105"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOI04864.1"
                     /translation="MQTANIPQHVAIIMDGNRRWARKQGLAIIKGHRQTAEETIERLA
                     DYAIKVGIKFLTLWAFSTENWNREQTEVEGIMNLFRETFSTATDRLHEKGIRIHTIGD
                     LSPFPQDIRAGIEKWKRETAKNNKLVVTFALNYGGRDEIVRAINRLITEKKNTKNQKI
                     TAEMFENYLDTRQEINLPDPELIIRPGGEQRLSGYLPWQGVYSELYFCDTLMPDFDEQ
                     EFAKALDAFAHRQRRFGQ"
     gene            complement(15005..15613)
                     /locus_tag="PLJ02_00110"
     CDS             complement(15005..15613)
                     /locus_tag="PLJ02_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOI04865.1"
                     /translation="MPTNLPNYDRVNLVNERDEIIASADKLAAHLGEAMLHQAISLFL
                     FRENLPGQFELLIQKRSQQKIVAAGQWGNTVCGNVAMGETHEQCLWRRLRAELGVELA
                     KDIKKQAKEVAVINYHVPCNSKYSEREIDHIFALYLNKQQLEKLTIQPHPEEVSELKW
                     LDWALLKRKKKISNLAIAPWLRLFLDKPEIVLAIDRYLANFS"
     gene            15691..18018
                     /locus_tag="PLJ02_00115"
     CDS             15691..18018
                     /locus_tag="PLJ02_00115"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOI04866.1"
                     /translation="MDKHYQAKENETKIYQLWEKADAFNPDSDINQKLKNDDLPSFSI
                     IMPPPNANDPLHLGHAAFLTLEDILIRHARMTGHDTLWLPGADHAGIETQFVFEKKLK
                     KEGTSRFHFDRATLYQMIWDYVATNSQIAIKQSKQLGASADWSRFKFTLDDDIVQSVI
                     ATFKQMLEDRLIYRANRLVNYCPKCGTGFSNLELDHREEIGSLYYLKYGPITIATTRP
                     ETIFADVAIAVHPKDQRYHKYHGQTIEYQGLFGLVKLLVIADDFVDPEFGTGAVKITP
                     FHDFNDFAFWQRHQAELKQAVQNNQLGYVELKEKLINNNQLSAEEINSPALAIDFTGR
                     LTKFTDQYAGLKAHIAREKIVSDLQEANLIEKIDNNYLHSVTSCYRCGSNIEPLPLPQ
                     FYVKVEPLTKAVLEKIKKKELTIYGAGHDKILKHWLENLEDWNISRQIVWGIRLPVWY
                     DAQENPQLRISFIDKNQARISGPIGELLTKYSFAEIEAGLQSLTAPIDAQFVVSQEKP
                     DPNRQYLQETDTFDTWFSSSQWPVVTLKNNQPGDFERFYPTTIMETAYDILMFWVMRM
                     LMMGLYSTQQLPFKKVYLHGLIRDSKGQKMSKSKGNVINPLEIGEKYGTDALRMAIVI
                     RSSAGLDKTLSEADFQAMRNLNNKLWNAARFILLQKENQAITDQNTAKLDKKFTKNLE
                     KIVLAVSRNLDQLQLGLAADTLYNEFWHWFCDQCIEDAKKGLISQEKLFDGLVIFLKL
                     FHPFIPFVTESIWQILYQEQLVAEPLLMTSRWPSC"
     gene            18024..18440
                     /locus_tag="PLJ02_00120"
     CDS             18024..18440
                     /locus_tag="PLJ02_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04867.1"
                     /translation="MSELISLFILIIKLIINLILFGAQYVIVKVKERKELTPLLVVIF
                     LILSLINWQLWQNHNTPKIEVKAAPSQNPGEILLTLEKEELEQLKNFYLALESKQKNS
                     RDILFNLGKILELEDPNLAQEKFQQAWDLDPNFSLR"
     gene            complement(18533..19258)
                     /locus_tag="PLJ02_00125"
     CDS             complement(18533..19258)
                     /locus_tag="PLJ02_00125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOI04868.1"
                     /translation="MTKTTFKVAKRSNVKAKKLRRQGILPANVYIPKKESIALEIDPH
                     AFAKLYEEVGETGLVYLHIDQEEQAVPALIDRVDMDYLGNSFEHVVFRGVNLKEKITA
                     KVPVEIVGEFDVPEAVLITVQDEVEVEALPADLPEKFVFDVSQLKAIGDTFTLADLKF
                     DNTKVELVLSEDEDPSERVLISVQAQQEEEPEAVVEELVEPEVIGEKEEAQTGESATE
                     GGATKEGEAKPAPTEKTAEEKSK"
     gene            complement(19472..22450)
                     /locus_tag="PLJ02_00130"
     CDS             complement(19472..22450)
                     /locus_tag="PLJ02_00130"
                     /inference="COORDINATES: protein motif:HMM:NF024594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HOI04869.1"
                     /translation="MLGQKKYLASELIEEQTTVFLLIEEENIFTKQLLVFFKNCGLEA
                     QPLLLSSFANPNQALKQYQLIKEVEVYKFLVVGGFKYNFFAQPEFLIQVLSALERAFV
                     EEGRVLPIGFLLNYSSALSPINIKLNNYDLFWSRQKGFLNSVLKNFPLAQVCLFEDYL
                     DLEFDFNLKFNLFFSLFKERLLLDVQTACFWQSQEGFWPNFRKIFFHGKAGEKYLLRG
                     KKTSSTDFLIAARDLCSRYFLEDFSLVKLFLQSNEEPVQLADFIKIYQSTDSVAQIID
                     QKIRQLPISLNETLMLTKKDLQSLKEQKIIVEPPLQRVEEKVVEEPEKAVSNSLESKE
                     LRPVVKETTKEETLKRSKEDKKVDISSDIDQKLQQIFRTQQKERHEKRFDKNLQGAKK
                     IVKKSKYRRVSFYFGVLLASVGSLVLVLFLSFFISQKSFEKNLLSIFRQGVEQGNSLQ
                     TTAEISKNYSFFEWQLQNYQKILGEDFLSTANNYWQIFAQIMRGQELAKTVTERTYNL
                     YQETLATDSNPEAAWVDFLTQKKELYQVKQQLSQYLEQLNPDILPDAQAENLIAYKAQ
                     LSKELRAEQKTTAFLEKLSDYLLSPSRSNIAILIQDSNELRSSGGFLSSVVILSFESG
                     HLLDWQVYDVGDLDQRVYGEKAISEELRNILVADKLLLRDANWPADFSQSGADIAWFI
                     KQSLSLNPDLILSLNTKELNALAAPLYPIVINGFTLQQNNFYEELLRQKNFNINDFTK
                     EFFARLTKLSMDELTHVFKQLLLALESREALMYSADNALITILKSNLWSGEILQTPCP
                     SEFAKNENCFTDGIYQLENNVGLNKVNRLITEKIEHNVGISENFIRHKRTVVLTNNSR
                     QNFWPEGNYQTYLKFYLPETARLEKFTLDGKNIDQGAYRWVKEGEKRVLAYRLLVPVM
                     SSSTLEIVYLIPHQLVTPFSYVFLDQKQAGIFDKTTNYKVLFAEQFRPNLIAPQANYK
                     NKVIEFTNNNQDNFLFAVAF"
     gene            complement(22485..22913)
                     /locus_tag="PLJ02_00135"
     CDS             complement(22485..22913)
                     /locus_tag="PLJ02_00135"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HOI04870.1"
                     /translation="MTTRLKVYTDGGSRSNPGMAGGGVYMEDENGQEFYREAKFLGIK
                     TNNEAEYLAFLMAVEYLMHYELKNPGQIQSVNFFLDSKLVVEQLNRRWKIKKTELKQL
                     AEAIWKNLSALPYSIEIKHVLREKNKLADELANQAMDRAC"
     gene            complement(22965..23426)
                     /locus_tag="PLJ02_00140"
     CDS             complement(22965..23426)
                     /locus_tag="PLJ02_00140"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOI04871.1"
                     /translation="MKIEEYVSKLIEHLGFEGEIEIKIEESDERVQINIQTDEEDTAL
                     LIGNKGETLEAIELLTKLSFKDDYPDKRIVLDINQYKLRQEERLKEKAISLAKRVLET
                     GRPYEFRYLNSYERHLVHETIANEVGLESLTTHSEDRDLGRVLLIEPKKNS"
     gene            complement(23435..24451)
                     /locus_tag="PLJ02_00145"
     CDS             complement(23435..24451)
                     /locus_tag="PLJ02_00145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HOI04872.1"
                     /translation="MEFFINFFSEVLLFLNEALGGVGLALIAFTIIIRLLLLPLTLPS
                     IKSSQKIKKLQPELKKIKEKYKDDKQGYALAQAQLYKSYNINPLAGCLPQIIQLLILI
                     VLYQAILRLFNQASDLNFRFLWADLSQPDNKFIFPILAALSQFVLSLMISPATQTRDI
                     VPNKTQSDLLKKANEKEEDIAEMASTMQQQMMFMMPIMTGIIALRLPSGLVLYWIMGT
                     LFSIITQYFVSGLGGIAVFWNNQIAKKGYFGAKLLPEEQGKNLLLQEEKKDKIVKEPL
                     LKKALKKGEATKETSELAQLLANKGGSTEALPAKNTRATKVKPQQKIKKNRQKKKHNK
                     KKRS"
     gene            complement(24461..24640)
                     /gene="yidD"
                     /locus_tag="PLJ02_00150"
     CDS             complement(24461..24640)
                     /gene="yidD"
                     /locus_tag="PLJ02_00150"
                     /inference="COORDINATES: protein motif:HMM:NF013932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOI04873.1"
                     /translation="MKLYKLLSTIRHNIMYSVFGYSPVCFQEPSCGQYTILEIKKNGT
                     IIGLTKGIWRVLHCH"
     gene            complement(24627..24998)
                     /locus_tag="PLJ02_00155"
     CDS             complement(24627..24998)
                     /locus_tag="PLJ02_00155"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOI04874.1"
                     /translation="MLAKKHRLNLSVAENSQLFTKSERFSTENLLFYYRTNQSHLKVA
                     ALAPTRLFNKAAERNHSRRLIYQLINQINRDHKIITKTNLLELKLDLIIVYKNNKAKS
                     EEIAKELKLFFDQILKKYEAL"
     gene            complement(25028..25174)
                     /gene="rpmH"
                     /locus_tag="PLJ02_00160"
     CDS             complement(25028..25174)
                     /gene="rpmH"
                     /locus_tag="PLJ02_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009142009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOI04875.1"
                     /translation="MQRTWNPKKKKRLRMHGFLNRMSSLGGRKVLAKRRAKGRKKLTV
                     IKKG"
     gene            complement(25196..>26011)
                     /gene="dnaN"
                     /locus_tag="PLJ02_00165"
     CDS             complement(25196..>26011)
                     /gene="dnaN"
                     /locus_tag="PLJ02_00165"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOI04876.1"
                     /translation="QSADEYPQFPEVSGNKFKLKAADLEKIQNLVVFASSNDQTRPVL
                     TALLMKFSNQGLTVVGTDGFRLASAVFPDLKFSFEQELLVPAKAITELLRIAKQSQAD
                     EVEVTVADELKQLLFKVDSVDFFVRLIEGDFPPYQKIVPPSFKLSVSIDSDEFLNQLK
                     RAQIFARETSNIVRLSLKNQDKSSFLVIKAISPSYGEYLSEILVELETDLGKDEIEEI
                     QIAFNAVYLIEFINAVHSEKIIMSINESLKPAQFISPENKNYFYISMPFRVNE"
BASE COUNT         8211 a         5159 c         4407 g         8234 t
ORIGIN      
        1 ataattttag cggaaatgct cgatatgcaa ttggctcaaa acgaagggat acccccgctc
       61 gaacaaatta tggagatgaa tctttacaaa acagcccgct atactttttc cctcccccta
      121 atgttggctg gtcatttagc tgatcaatca ccagcaatga ttaataattt gagtgaaatt
      181 ggtgaaaaag ttggtttaat ttttcagatg agagacgatt atttaggtat ttttggggat
      241 aataaaaaaa ccggaaaacc cattctttca gacataaggg aaggtaaaaa aactattttt
      301 tattattatt taattaaaag cgaacttctc agccccaaag acaagcaatt gttaaatagt
      361 tgtttagctg tcgaacaaat cagtcaagaa aatagtttaa ccctacaaaa actatttaaa
      421 aatcatagtc tgccagcggt taatcagcaa atgaaaaaac tcgaagatga aacattaggc
      481 attattgata atttaaataa tgacaaatta gcaaaattac taaaagaaat tttaaatttt
      541 gctcaatatc gccaatgcta atgctgataa ttaaaccttg ggatcttgat gctcttttac
      601 caacaaggtg agcaacaccc caactgctgc cactaaaaat aggcttatcc aagcttcacc
      661 aaaaaaataa aataagaaaa aaaccaacga ccaaaacaca atcgctgctg cgctagtaat
      721 catttgccga taagtaaaat atgataaacc ttcccgacct ttacctctca ctacccaaac
      781 tcgatcaaaa aataaccaat gaaaactaga ggtgatacga tccagcgtat tcaccacgac
      841 aattgaccaa aaaccaacga caaaacttct aatgatccaa aacaaggata agattcctgc
      901 tgacaaaaca aacggtcgtc gacttttatg tttatcaatc actgctccca ccccataact
      961 aatcaccatg gctaatagta gtgaaaaact atataaaaaa ccaactttgt cactagtgcc
     1021 cagtaagaga aaaatataca aaggccacat ttgtaataca ccatcattaa aatagctccc
     1081 agcaaaagtc agtcccagcc gtctaaaacc tggttccatc atccaagtag agaattcttc
     1141 ccaagaaatt ttgtcatgga ctttaacgtt atccatggag attgaaaaaa tgagaccaat
     1201 agtaattaat actaatccta gtaaaaatag attaccgtaa ccaagacccg caataatcaa
     1261 accacccaaa gcgggagaaa tcatagtaat gatattaagc aaaaaattaa taatccctaa
     1321 attactccct attttatgat gctcgctgtt gcgacttaac gaataataat aactattcca
     1381 aaaataatta gtttgcactc catcaataat cactgccaaa aaaatccaat aggggttaat
     1441 ttgcgataaa aataagaaag cggcaaataa aacatacaaa atttgtccca tgacaaaggc
     1501 gtgttttgtc cctagcttaa tagtccattc cgctacccaa attgccgaaa gaaaggccac
     1561 caaacgatct agtagctgga aaagcgcaat attaaagaca ccaatttgca gagcagttaa
     1621 agtattttgc ccattgaggc ttaagctcca attaatttta tcccagaacg gtaagtgcag
     1681 attgaaatag taaattggta aaaaaaacat caccaattta ttaaccaatt cacgcattaa
     1741 acggattgcg tacaaatgct tgagattttt gtttttctgg aaagctaact tgggcagttt
     1801 cattactagt tcagtataaa ctaactagag taactcctgc aaaagttgtt taacactttg
     1861 atcaaaaatt gcttgttgct cgggtggcat cctttgcaaa acatagtcag caggagcgat
     1921 atttcttaaa ccatttctag catctatgcc cagacgcaga tgccaaaagt cttttgtccc
     1981 taagtgttga taaattgact gcaaaccatt gtgctctttt ggaccagtcc ctttttgtaa
     2041 tttgtatgtt cccagtggca aatctaaatc atcatggaca acaatcaaat cctctgggcc
     2101 agcaaaagga tcttgcagat caatttttaa atagtctaag aaagctctta atgcttgacc
     2161 agaattgttc ataaaagttt gtggcttaat taaaaacaaa tcatcccttt tgactgctaa
     2221 ctgcgcatta aattttttgt caaatttaaa agttaaggat aaagcttcgg ccatagcctc
     2281 aacaaacata aaaccagcgt tatggcgagt gccctgatat tctggcccag gattacccaa
     2341 acctacataa attttcatca gtcttattct aacatttaac tttttttata ttgtttacag
     2401 ttataatatg cactgtgaaa aaaaatattg gcatttttgg cagtaatcaa ggccaccaaa
     2461 gtattgctga agccattaaa gaaaaaattg aatcacaagc gggcgatcgt tatcaagttc
     2521 atattctcta taccaaacag ccactttcac taatttataa ctcaatttat cgatttgccc
     2581 caaagatttt tgatcgtccc tacgaatttt cggctaatat ggttgaaaat tatgccgaat
     2641 taaacgatct agtctctagt ttttttctta aaagcactta tcaattagct aaggatttta
     2701 ttgaagaaaa aaacattgat ttaacaatca gtacttattt ttcctttcaa cctactttgg
     2761 aaaaaattca acaagaaaaa cagattcctt acttcaatgt aatagctaac ccgagaacta
     2821 tccatcctct ggaaatatct cctcaggcta aaattaactt ttttttcgac gacaaaaact
     2881 taacagaata ccaaaaaagg tgtcgagcta aagtcacggg ttggtttgtg ggtaaacgtt
     2941 ttgaaacaac ttaccaaaaa gaagcggtta gaaaaaaatt aaaaattaca ccagctctca
     3001 cttttttaat tgcctctggt tcagagggtt ctactgccgt tttaaaaatc ttaccagctt
     3061 tgattaattg cccaaaaccg ctgcaggttt ttgtagcttg tggtaataac aaaaatttat
     3121 atagcaatgt attggggatt caaaaaagct tagttgcctt tagtcaatct caagccatta
     3181 ttacgccatt aaaatttact aaaaatttac atctatacat gcaagcagcc gatttaatta
     3241 tcggcaaagc cggacctaac actttgtttg agagtgtggc aactatcact ccatttttcg
     3301 ctattactca ccttgctcaa gaagaaggta atttagcaat tattaaagaa cacaatctag
     3361 gcatcgtagc agaacaatct caagcagcta atcaaaaaat ttccgctatt attcgacaac
     3421 ccaagcaatt ggcaaaattt aaacctggtt tgcttaacat caaacaatat aatcaacagt
     3481 ctgcttccat tttaattgaa gaaatcgata caattttgca aaaatcagtc aaatgaaact
     3541 gaccgcccat ctctaagcaa ctaggcttga cgaatttctt ttaattcttg aagcgtggtg
     3601 gcttgttgcc aatttttatc ttgacgaatt gtcactaagc gaggaaaacg tagcgctact
     3661 ccggccgtat gactgggaga ttgagaaatc tcatccgcag caatttctaa aactaggttg
     3721 ggttcgatcc aaacatcagc ctttaattcc tttttaaaat catattgggg attagcttgg
     3781 ttttggggac ttcggtattg atcagctaat tgtttaattg ctcgaaactg gtcgtcggtt
     3841 aaaccagtac caattttgga caaagtttta attttaccag tatcttgatc taaaacgccc
     3901 accaacagcg caccgatacc aaaactttgg cgcttgcctt ttccccgata ataaccaagc
     3961 ataatgcaat ccagcgtgtc attcaattta cctcgttggc cttctgcttc tttaattttg
     4021 acccaacgcc aacccttacg accagcaaca taagcagatt ctggtttttt catcactgct
     4081 ccctctaaac caacagctaa ttgtttttgg tgaaaatcac ttaaggtttg aggatcatta
     4141 attagctggt aaatagtttt tttaacgaca gttgaatcct gtatggcttc atccagcaat
     4201 tttttgcgat caatgagggg ctcatctatt aaagcgcgac cattataaaa aagtaaatca
     4261 aaaacataaa agcaaattgg agtttcttct gctttaacat ccacctgata tttgcgtttt
     4321 ctcgaaatag tttcttgaaa aggtaaaaat ttcttggttt ggggatcgta agcaatggct
     4381 tcactatcaa aaatagctgt ttgagcctgg cattgtgtta acacttgctc taactcggga
     4441 aacatcgccg acacttcttc taaattccgg gtgtaagcct tgtagaagct accatcagca
     4501 aaaccatttt tattaatgtg aatttgaatc cgtaaaccat catacttcgg ttcagcaata
     4561 acttcgccca tcttgtcaat gatttcttga ccactattca agcgctgaca cagagccggc
     4621 acaactggga tacccagttc taactgatag tttttagcga gttctttggc gcttaagttt
     4681 tcatcgaata aatataattg agccaacaaa ccaacatcag cctttttttg aaaagcttct
     4741 tccaacagcg ctgactgact tttatctcca gtttttgccc aagacagcgc gtcgagcatc
     4801 gtcatcgtcg aaaaacccaa gcgcattttg ccaagaatga tgcgggcaat aaactttgct
     4861 gatagtgcgc tgacttgatc caagagatca gctaataaat taatttttct gtcttgtgaa
     4921 ccttcgcctt tttctccagc aatcaactct agttgctgat gaacttcaac aattgttaat
     4981 gcctcttctt gcccatcaac tgttgctaaa tcactcacaa cttgataaaa cagctcacct
     5041 aaatctccaa ggtttttata ttttttggtt aattcactaa caattgtttg ctgatcatct
     5101 tgatcaaata aaccttgggt gaataatgat tggtgatcta catctggttt cgcgtggcga
     5161 gcataaaaac tagctaaaga gcgaattaac attttgttgg ataattgaaa ttctaacgat
     5221 cgataattag ctaccaaact gccttggatt aaataactag ctggcactat ttcatctttt
     5281 cccaattttt taaataattg ggctaataat tcagtcatct ctaaacgaga gctaattttt
     5341 tctaaatcgg ctaaatattg ggcaaattga tcgaaaatca taaattactt tcaaaacata
     5401 ttatgtcaga acaattagaa aaaccaagtt aaactcatta tcaaattcaa cattatcatt
     5461 taactaaggc tgtaactaac tcccttagtc acaccatgtt tcttaaccac acctttagca
     5521 attaaatctt ttaaatctcg caaaatagtg tcaaccgaca cgttaggtag cactttttgt
     5581 gcatcatccg aggtcatcgt gccctggttt tgtaatagtt ctaacaaaat aatttgtcgt
     5641 tcagacaacg ccacttgttg acccaactct cgctttaatt tcaaatcctg agacagttta
     5701 gccactttgg cttttacctt atcaatttcc agagccaaac cataggcaaa atattctaac
     5761 caataagtta aatcttgttt gggatgttgc tggaccgata acagagcttg ataataagca
     5821 tcaacatcac tgtcaaaata ttcttctagc gaaaagaaac gtttaaaatc atagccagcc
     5881 tcataaagtg ctagtgtagc aaaggctcgg gctgtacgac cattaccctc aacataagga
     5941 tggatataaa ccagctgata atgaataata gctgcccgta aaattggatg aaggttttga
     6001 ctttcttcac tgtttatcca agcaaaaaga tcatctagca aaaatggcac ttcggctgct
     6061 accggaggac gaaagacgac ttgaccattt ttagctcctc ggacaatgac ttgtttgttg
     6121 cgcaaagtag ctatttgttc ttcatcgagc aatttgtcca tagtcaggct atgtaacaaa
     6181 ttcagtgtac ttaaattaag taatgaagat tgttgatcag tatttttttt caacaaaggt
     6241 tgctggtcaa tccaagctaa aacattacgg taattaatta cctcttggat atctctttcc
     6301 cgggcaataa tccccgtctg atctaaaaca gtttgcacat catcggtttt gcctaaagac
     6361 agtacttgac ttgcctgatc agcgctcaaa tcattaccct caattttagt gccataatga
     6421 acactgcgcg tcaacgcttc atcacggaat ttggcttccc aagcaggtac cagaggtgag
     6481 ttatcaatga cggccttagc cgactcaata gcggaaatat aggttaataa attattacta
     6541 attcgataat gaggttggta cattaaagac atattttggc acagtttgat agctaagaca
     6601 agagttattt ttgcaaaatg tgcaaattta aagaaaaact ccaggtcaaa taaactagct
     6661 acctaattgg cttctaaaaa gttatgtcag tgaagcaaaa acatagtaaa atgaactgat
     6721 gattgaaaat cctcaacaaa gtgttcaagc ccaacaattg attgaccaag cctttcacaa
     6781 cttaaaaatt gctaaaaaag actcacacaa gggtcaaaat ggtaaattat tgattattgg
     6841 cggatccaaa ctttttcatg ctgcgagtaa atggagttta gaagttgcta gtaaaattgt
     6901 ggacatggtt ttttacagtt cggtgccaag taataatcgc ctaattaaaa agtggattag
     6961 tgccgaatta gaccatcaat tgacccaagc agttaaagag aatttctggc aaggaattgt
     7021 cattgatcgt cgacaaattg aagattatct ggatgaagcc gattgcatct tactaggacc
     7081 aggtatggag cgaaccaact atactgccaa gttaagtaat aaattactga aaaaatttcc
     7141 ccagaaaaaa tgggtcattg acgctggcgc cttacagatg gttgacccac atctactcag
     7201 tcaaaactgt attattactc ctcatcaaca agaaatggac attttagtcc aaaagaatca
     7261 aggttttgct ccaaataatt atcaagctct gcccatttgc ttacttaaag gtccagttga
     7321 tcgaatcctg atttccaaga atcattcact ctcaaccaaa acaactctga tagaaatcac
     7381 gggtggcaac gctggcatga ctaaaggtgg tactggcgat gtcttggccg gtttattggc
     7441 cgccctttat tgccaaaacg atgctttgac tagcaccatt attgccagct acatcaataa
     7501 aaaagctggt gattctctct ataaaagtgt tggcccttat tttaatgcca gcgatttagt
     7561 ggcaaaaatt cctcaagtgt tgtgggaaac gcttcattca agagaaagct tgaaaaatca
     7621 agaaaatcaa gtagaatacc tacattcgtt tggcgatagt cttattattt tggcaagaaa
     7681 gagatcagga aaaacattat agcaatgact aaagaagaaa cagtagatca taatccggtt
     7741 agccaccccg acctcggtga tagtagggag ggcagtaata atagtgaaaa tggtgattac
     7801 gacgatgacg gtcacggcaa tgatggttat gccaataatg atcataaagt tagcagtgaa
     7861 cagaacaaaa cagcaacaat agctgaaaat ccttacagca ttccaccaat ttttaataag
     7921 tataaaaaca tgtctttaga cactttcttc aaaatacttg acgctaagtc gaaaaaacca
     7981 caagaaattg atgatgatct cgatgttgtc ttgacttttt taagagattt agaaaattcc
     8041 attaaaattg cccaacaatt aacggatgct catggcaacc cttgcttccc agcactgtat
     8101 gaacaacatt gctcacgagc tgaatatttg agtaagttgc ttgagcaatt tcgctggttt
     8161 actgaattga aaaaaaatcc tcgttttgat atttctacca ttggacacaa tgggtataac
     8221 aattctaaaa aagataataa caaaacagtt ttttaaactg acttcaattc aattaattga
     8281 ctctgctgga ttttcttgct caatgccgta gctaaatcac cgtcggctgt agttaacacc
     8341 acctgataat tttctaacaa agatagcacc attgctttac tactttcatc caattcagac
     8401 ataatatcgt ctaataacaa tagcggttga taatcaatcc gactgtgtaa aaactgtagt
     8461 tcggcaattt tcaaccacaa cacccctaaa cgtcgttgac ctcgagaacc ataagctaaa
     8521 aggctcaaat cttcaattcc tttttcgggc aaggagaact taaccaaaaa atcgtctttg
     8581 tggggaccaa ttagggtatg acctacaaca atttcttgat cagcatattt ttttaagcgc
     8641 tcttcattaa tcagagaagg taaatattca atattcaatt caaagggaaa aatcacttct
     8701 cttataaaat caacaaaatg gctacgctcc cgctgaagaa attttccgtg ttcgagtaaa
     8761 ttggtgttcc aaaaagataa gatgcccttg ctctcttcgc cttctcgcac tttttgcagc
     8821 agcaaattac gccgtttcag agtttgttca tactgttgaa gagcacgttg atattcccaa
     8881 tctaacattg acagggcttg atctaaaaaa aggcggcgcc ggctgggact accttcaatt
     8941 aatcgtaaat cttccggacg aaataccacc actgacaaat ttttgacaaa atctttgcgc
     9001 cggcgccgca catcattaac agtaaacaag cggtattgac tccgcctacc agccaccatt
     9061 cccctggtca acatgacctc taattttaaa tcatcagctg ttgcttcttt tttttcgccc
     9121 aaccaatctt tttctgccaa cgaatctttt ttttccgacc aatcattttc tcctgaccag
     9181 tctttttccg caaacaaatc ttcttcattg ctcaaggaaa ataatccttg gactctactt
     9241 agctctgctt caagccgaat catttcttca acatgtgtcg ctcgaaaact gtctccagta
     9301 gccaaaagat tgatcgcttc aacaatcgaa gtctttccac taccatttgg cccaacaaaa
     9361 attgtcgttc taccgaagtc ccagttctgg ttggtaaaat tccgaaaatt ttgtaaagct
     9421 aatttacgca atagcatcgt tttattcgtc actgaccatc attttacaac taatgacaat
     9481 taattatgga aagcccgttt aaatcgccaa aataaataaa tgagcaaaat ttgccaatat
     9541 aaaaagaaaa agaaaaaaga cctgtaaatc aatctaaaaa ctattgatcc atcataaaac
     9601 aagtctaaca accaccaggc aaataaaaga taacaaatag tggccaataa agtaggtaaa
     9661 aacatcaaag ggacaaagac cttatcgcga actaaattct gcatgctctg ataaggatgt
     9721 aaaaataaac ctaaaacaat ttggcccaaa gaataaatta atgattcaac aatagctaat
     9781 ttatccattg tttccttgat ataaaaacag ctcccaaacg agagaactga ttaaagctaa
     9841 aaacaaaatg atctgccatg aatgggtagc cacaaagtta gcaagagtac taaaacgatc
     9901 aggaataaac ttttcttttt tcagagaact aataatttta gcttcagttt ttaattgctt
     9961 taaaaattct tgcttatctg gcatagttgg tgcaaattta tcatatcaat caatttattt
    10021 ttcaagattt gcccaaagca aacgaaactc ttttctagct ctaaataatt ctgattcaat
    10081 tgccttttca ctacgaccaa attcttgagc taattctttc actctttttt tatcaacata
    10141 ttttttcatt aataactctt gcgattggct acccattttt tttaatactt gcttcactaa
    10201 aatggcagtt tcatgggcat ctttgtattc atgatccaac aaaaactctg aaagaggaat
    10261 agttttaata aatttttttg catagcgttt ccgataaaaa tcattgattt catgacgcaa
    10321 aatactttgc atgtaagtta gtagggaact atctccctta aacaaagtaa tactgcgcaa
    10381 gcagttgata aaagtttctt gaactatttc ttcagctata gagcgattgg gcaacttctg
    10441 catggctaaa tttaacaatt gtttgtggta atttttgaac caatattcaa tcgcagattc
    10501 gtggccattt ttaatcgctt caaccaaaac ttgctcgtta ctaaatccaa gaaaacgttg
    10561 cataagtaaa ctaccctatc atatcagaaa attttatttt ataattacgc tgatgaaaaa
    10621 gacaattact ttattttcat tagttttatt catttttgtt gcttatcttt ttctcactca
    10681 aaagggaatt ttaactccta acgtcattta tgctgaaaca gaaaatgtag aaattgatct
    10741 ttcagaacac ttaacacttg gcaatggcca aagattagct gaacaatttg caacccctgc
    10801 cagtatgatt aatttattaa ttagagtagt ttttgttgga gtaggtttaa ttttattagc
    10861 catgattatt atttctggct tatcaatgat tgctgcaggc acaactgaga gcaaagataa
    10921 agcgagtacc actatgactt ctgcactaat ggggtttttg gtaatttttg ccgcatattg
    10981 gatattacag attattcaaa tatttactgg ggtcaatctt ggcttttaaa aagtaactag
    11041 atataataaa tacttataat gatcaaaatt aaaactataa aattgttgct tccattgctt
    11101 ttttttctct tattccttcg acccgtcaag gctcaaacta ttattccttg tgggacacca
    11161 ggcattaaga aatgtactac gaccgaaact ggtcagccag cttatgcggt gtgcgcagct
    11221 gatggcatca cttggaaacg atatgcttgt acagcaggaa gagtttgctt acctagcgat
    11281 cccagttgtg ttgatcccag tgagcaccca gaggcagttg tcggtgcaat cgacgctccc
    11341 ccaggagtag ctaaattcaa tgctcaagct gacagtggtc ttggtattat tctgttcttt
    11401 tctaatttaa ttaaattaat tgctattgtt gctggtttat ggacaatgat taattttatt
    11461 attgctggtt ttacttatgt aacttcggcc gataacccta gtgccataga aaaaattggt
    11521 tccaagttaa gttctagcgt aataggattg gctattattg ttgcctccta tactattgca
    11581 gcagttattg gtttaatatt atttggtgat gctggtttta ttattagtcc agaaatacca
    11641 actgtagctt tgccttaaaa ttattaaatt aaagaaatta tgtcaaatat ccacactctc
    11701 atcgctcaaa acagtgatca gtctattttt ggcactatca atgctcctca gggagttgcc
    11761 gagtttaacc aacaagccgg aaacattggc attattctgt tcttttctaa tttaattaaa
    11821 ttaattgcta ttgttgctgg tttatggaca atgattaatt ttattattgc tggttttact
    11881 tatgtaactt cggccgataa ccctagtgcc atagaaaaaa ttggttccaa gttaagttct
    11941 agcgtaatag gattggctat tattgttgcc tcctatacta ttgcagcagt tattggttta
    12001 atattatttg gtgatgctgg ttttattatt agtccagaaa taccaactgt agctttgcct
    12061 taaaattatt aaattaaaga aattatgtca aatatccaca ctctcatcgc tcaaaacagt
    12121 gtcaatattg gttctttaaa acctgttact gaagcgcgcc aaccagaaaa tttagaaact
    12181 gtcaccgttc tttccaccct agaactatgg atcagcgata ttattggtgt cctcaccatc
    12241 ctcggtactt tgttctttat cgtttatgcc tttatctctg cctttaacat cattaccgct
    12301 tctggagata aaggtaaatt agaaaaggct cagaaccgtt tggtctggag tacgcttggg
    12361 cttattctaa tggtggctac ttatgctatt attggtttaa tcggtagtat tattggtttg
    12421 gagttgcttc aaccaggtgc catgttacaa acaattatac ctacagggat tcaaccttaa
    12481 gcagaaatta ttaacaagcc ataagactaa taatgaaaaa aaatctcgtt tacctaaatt
    12541 tagcagtagt tgtcacgctt ttattttttc tattgacacc gagcgttcta gcccaaactc
    12601 aaggaattac caaccccgtc attggtgatc tgggaaccaa cgaccctagt gtttctagcg
    12661 gtgggagatt tatccagtac acggtttact tgtggcgggc atctattaca cttggctcgc
    12721 tagcagtaat tgccttcttt ttacttggtg cttttgagtg gatcacttct ggtggagata
    12781 aaactaaagt tgaaaatgct cgtaataaaa ttaccagtgc cgtcattggt ttggtattac
    12841 tcgttagttc ttttgtttta ttaagttttc tgagcaaact tttgtttgct ggagaatttg
    12901 atttgttaaa attaaccgtt cctggaataa attaaaaact aatcaataaa tcaatacaaa
    12961 gcaacactat gcaccaacaa ctagcacaag tcactttaga tatagcaggt gaagcacaaa
    13021 aacaaggact tcttggagct gaacaagccc aaaccgcaga tggtgggttc gggaattggt
    13081 taagtaatat tcttactgtc gtcatggttc ttggagctgt cgtttgcttg gccctaatta
    13141 ttatgggggc cattcaatgg atcacttctg gtggagataa ggcaaaaact gaagaggcgc
    13201 gtaataggat tactagtgcc attattggtt taatcgtttt agctgctaca atagctcttt
    13261 ttagcctggt agctgacttt cttggcatta cggcaataaa attcatctag atttttttgc
    13321 taaagcctaa taaacttgat ataataacaa ttagataaaa aaaattaaaa ggagactttt
    13381 atgaaatcaa ttaaaaaatt tgctttagct tctgctagtg ctttaagtgg attactggtg
    13441 ccaataaaaa tggccatggc cgcttttgat gatattgaga ttaatccagg aactggatat
    13501 gctactgatt ttggcaatat gttcagctcc attttaaacg tggttatgct aatagctgcc
    13561 gtcttagttt ttgcttttct gatttttggt ggcatacaat ggattacggc tggcggagac
    13621 aagagcaaag ctgaagaggc gcgtaataga attacttccg ccatcatagg cttagttatt
    13681 gttgctgctt catatgctct catcaactta atagttaact tcctaggctt tggtagcttc
    13741 aacgatgttt tcaagaatgt tggcactatc aacgacgcca actagtttag tttagaagca
    13801 aaaacccccg atcaactttg aaagggggtt ttttattgct ataatctagc tcatgaataa
    13861 aaacttcgcc ttgttacctt tagtcaaacc tgtccaagca caaacagaat cttgggaaga
    13921 catcaatcct aattgtgtag ttgatggcac cgccaccatt caaggaatcg gttgtctgtt
    13981 agccaatgta ttgtctgtta ttttaaccgt tataggttta actggttttg tcatgattat
    14041 ttatgctgcc tttatcatgc taacctcggc cggtaaaagc caacaggttg aaaaatcaaa
    14101 aaacactatt actatggcca tagttggcat aatcatcgct ctatcctctt ttattattat
    14161 caatctcatc gccaacttta ctggcatcag catttttaaa acgctgacca tccccggatc
    14221 aagcaccact tggtaaaaaa ccgttcgaat tactgtccaa atcttctttg ccgatgagcg
    14281 aatgcatcga gcgctttggc aaattcttgt tcatcaaaat ctggcatcaa agtgtcacaa
    14341 aagtataatt cgctatacac gccctgccag ggtaggtaac cagacaaccg ttgctctccc
    14401 ccgggtctaa taatcaattc tggatctggt agattaattt cttgacgggt atctagataa
    14461 ttttcaaaca tttccgctgt aattttttga tttttagtgt ttttcttttc agtaattaag
    14521 cgattaatgg ctctgactat ttcatctcga ccaccataat taagcgcgaa agtgactact
    14581 aatttattat ttttggccgt ttctctcttc catttctcta tgcctgcccg aatatcttga
    14641 ggaaagggac ttaaatcacc aatcgtgtga attctaatgc ctttttcatg taaacgatcg
    14701 gttgccgtac tgaaggtctc acgaaacaaa ttcatgattc cttcaacctc tgtttgttct
    14761 cgattccaat tttcagtact aaatgcccat aaagttagaa atttaatccc aacttttata
    14821 gcataatcgg ccaagcgctc aatcgtctcc tctgccgttt gccgatgacc tttaataatc
    14881 gccaatcctt gctttctggc ccagcggcga ttgccatcca taataattgc tacgtgttgg
    14941 ggaatgtttg ctgtttgcat aattttaatt ttattacttt tttgatttag ccttggcaaa
    15001 ataatcagga aaaattagct aaataacgat ctatggccag aacaatctct ggtttgtcga
    15061 gaaacagcct tagccatgga gcaatagcta aattgctgat ttttttcttt ctttttaaaa
    15121 gcgcccaatc aagccatttt aactcactca cctcttcagg atgaggttga attgttaact
    15181 tttctaattg ctgtttattt aagtacaaag caaaaatatg atcaatttct cgctcactat
    15241 atttgctgtt acaaggcaca tgataattga tcaccgcaac ttctttggct tgttttttaa
    15301 tgtccttggc caactcaaca cccaattctg ctcttaagcg tcgccataaa cactgctcat
    15361 gagtttcacc catggctaca ttgccgcaaa ctgtatttcc ccactgcccc gctgccacaa
    15421 ttttttgttg actgcgtttt tgaattaata actcaaactg tcccggcaga ttttctctaa
    15481 ataaaaataa agaaatagcc tgatgcaaca tagcttctcc caagtgagct gctaatttat
    15541 cggcactggc aataatctca tcccgttcat taaccaaatt aacacgatca taattgggca
    15601 aatttgttgg catctgtgtc attttagcag agaacaacaa aaacatataa actagagtca
    15661 ggctggcttc gatgctataa tcaaaagcca atggacaaac actaccaagc taaagaaaac
    15721 gaaacaaaaa tctatcaatt gtgggaaaaa gctgatgctt ttaacccaga tagtgacatc
    15781 aatcaaaagt taaaaaatga tgatttacca agcttttcta ttataatgcc tccgcccaat
    15841 gccaacgatc cactccattt aggtcacgcg gcatttttaa ctttagaaga cattctgatt
    15901 cgtcacgctc ggatgacagg ccacgatact ctctggcttc ctggagccga tcacgccggt
    15961 atcgaaactc aatttgtgtt cgaaaagaaa ctgaaaaaag aaggcaccag tcgcttccat
    16021 tttgatcgag caacgctgta tcagatgatt tgggattacg tggcgacaaa tagccaaatt
    16081 gccattaagc aaagcaaaca attgggcgcg agcgccgact ggagtcgttt taaatttact
    16141 ttggacgacg atattgtgca aagcgtcatc gccactttta aacaaatgct ggaagatagg
    16201 ttgatttatc gggcaaatag attggtaaat tattgcccaa aatgtggcac tggtttcagc
    16261 aatctggagc ttgatcatcg agaagaaatt ggttcacttt attatttaaa atatggcccc
    16321 atcaccattg ccaccaccag accagaaact atttttgccg atgtcgccat tgctgttcac
    16381 ccaaaagatc aacgttatca caaatatcac gggcaaacta ttgaatatca aggcctattt
    16441 ggcttagtaa aattattagt cattgctgac gattttgttg atccagagtt tggcaccggt
    16501 gctgtcaaaa ttacgccgtt tcatgatttt aacgactttg ctttttggca aagacatcag
    16561 gctgaactca agcaagctgt acaaaataac caattaggct atgtcgaact caaggaaaaa
    16621 ctgattaaca acaatcaatt gagtgctgaa gaaattaatt ctccagccct ggcgattgat
    16681 tttactggca gattaactaa gtttactgat caatatgccg gactcaaagc gcatattgcc
    16741 cgagaaaaga ttgttagtga cctgcaagaa gctaatctga tcgaaaaaat tgataacaat
    16801 tatctacaca gtgtcactag ttgttacaga tgtggcagta atattgaacc gctacctctc
    16861 ccccaatttt atgtcaaagt cgaaccatta accaaagccg tgctagagaa aatcaagaaa
    16921 aaggagttaa caatctatgg agcgggtcac gacaagatac ttaagcactg gttagaaaat
    16981 ctcgaagatt ggaatattag tcgccagata gtttggggca ttcgtttgcc agtttggtac
    17041 gatgcacagg aaaatcctca attaagaatc tcttttatag acaaaaatca agcaagaatc
    17101 agtggtccaa tcggcgaatt attaacaaaa tatagttttg ccgaaatcga agcaggacta
    17161 caaagtttga ccgctccgat cgacgctcag tttgtagtta gccaggaaaa acccgaccca
    17221 aacagacaat atttgcaaga aactgatacc tttgatacct ggttttcttc aagtcagtgg
    17281 ccagtggtca ctttgaaaaa taatcagcct ggtgatttcg agcgcttcta cccaaccacc
    17341 atcatggaaa cagcttatga cattttaatg ttctgggtaa tgaggatgct gatgatgggt
    17401 ttatacagta cccaacaatt accatttaaa aaagtctacc tgcacggcct aattagagac
    17461 agcaagggcc agaaaatgag taagagtaag ggcaatgtca tcaatccctt agaaattggc
    17521 gaaaaatacg gcacagacgc gctcagaatg gctattgtca ttcgatccag tgctggttta
    17581 gacaaaaccc tgagcgaagc agattttcag gcaatgcgta atttaaacaa caaactgtgg
    17641 aatgcggcac gatttatttt gctacaaaaa gaaaatcaag ccattaccga ccaaaacacc
    17701 gccaagttag ataaaaaatt tactaaaaat ctggagaaaa ttgtcttggc tgtcagtcgc
    17761 aatctggatc agttgcaatt aggcttagct gccgacacct tgtacaatga gttttggcac
    17821 tggttttgtg atcagtgtat agaagatgca aaaaagggat tgataagtca agaaaaatta
    17881 tttgacggtt tagttatttt tttgaagttg tttcacccgt ttataccctt tgtcaccgaa
    17941 agtatttggc aaattctcta tcaagaacaa ttagttgcag aaccattatt aatgactagt
    18001 cgctggccat catgttaaaa agagtgtctg aattaatatc actatttatc ctaatcatca
    18061 aactgattat caacctcatt ctgtttggcg ctcaatacgt catcgttaaa gttaaggaac
    18121 gaaaagaatt gaccccgcta ttggttgtaa tttttttaat tctttcttta attaattggc
    18181 aactctggca aaaccacaac acccccaaaa tcgaagtcaa agcggccccg agtcaaaatc
    18241 ctggcgaaat actgttaact ctagaaaaag aagaactgga gcaattaaag aacttttatt
    18301 tagcactgga atcaaaacaa aaaaactctc gtgatattct ctttaattta ggtaaaattc
    18361 tcgagcttga agatccaaac ctggcccaag aaaaatttca gcaagcatgg gatttagatc
    18421 caaatttttc tcttcgataa tttttccccc accccgctaa acagctaaca acaaacaata
    18481 attataaaca tgaaaaatat tcagttagaa ttctcgggtc taaaaaggcg ttttatttag
    18541 atttctcttc agcagttttt tcagttggag ctggcttagc ttcaccttct ttggtggccc
    18601 caccttctgt ggcagactca ccggtttggg cttcctcttt ttcaccgatc acctctggct
    18661 caaccaattc ctcgacaact gcttcaggct cttcttcttg ttgagcttgt acagaaatta
    18721 acactcgctc gctcgggtct tcgtcctcgc tcaacaccaa ttccacttta gtgttatcaa
    18781 actttaaatc ggcgagggta aaggtatcgc caattgcctt caactgacta acgtcaaaaa
    18841 caaatttttc tggcaaatca gctggcaacg cttcaacctc gacctcatct tggacagtaa
    18901 ttaaaacggc ttctggtaca tcaaattctc ctacaatttc cactggcact ttggcagtaa
    18961 tcttttcttt taagttaact cccctgaaca ccacatgttc gaaactgttg cccaaataat
    19021 ccatatcgac tcgatcaatt aaggctggca cagcttgttc ttcttggtca atatgcaaat
    19081 aaaccagtcc agtctcaccc acttcttcat aaagtttagc aaaagcgtga gggtcgatct
    19141 ctagagcgat tgattctttt ttgggaatat agacgttagc tggtagaatc ccctggcgac
    19201 gtaatttctt agctttaaca tttgagcgct tagctacttt aaaagtggtt ttagtcataa
    19261 ttaatttcct atttttatcg agaatttttc attaaaaaat ccaagcggat ccctattcta
    19321 ccacaaagac cataaaatct ctaaaaagat tatggccaaa caaagagcga acaaaagatg
    19381 tcagtaatat tttgcttaag atgaaaaaca ttattaaaaa agtatggtaa ttcaacgtat
    19441 tttcaaaaaa agggcttttt tacacgtgaa attaaaaggc cacggcaaac aagaagttgt
    19501 cctgattatt attagtgaat tcaatcacct tatttttgta attggcttga ggtgctatta
    19561 aatttggccg gaattgctca gcaaacaaaa ctttataatt ggtggttttg tcaaaaattc
    19621 ctgcttgttt ttggtccaag aacacataag aaaatggggt gactaattga tgcgggataa
    19681 gataaacaat ttctagagta gaacttgaca tgaccggtac tagcaaccga taagctaaaa
    19741 cacgtttctc tccttctttt acccagcggt aggcaccttg atcaatattt tttccatcta
    19801 aggtaaattt ttctaaacgc gccgtttcgg gtaagtaaaa tttgaggtaa gtttgataat
    19861 ttccctctgg ccaaaaattc tgacggctgt tatttgttaa aacaacagtg cgtttatggc
    19921 ggataaaatt ttcactaatt ccaacattat gttcgatttt ttccgtaatt agtcgattca
    19981 ctttgtttaa tcccacgtta ttttcaagct ggtaaatacc atcggtaaaa caattttcgt
    20041 ttttagcaaa ctcactagga cacggtgttt gcaaaatttc tcccgaccat aagttgcttt
    20101 ttaaaattgt gattaaggca ttatcggccg aatacatcag cgcttcccgg ctttccaagg
    20161 ccagcaatag ttgtttaaaa acgtgagtca gctcatccat tgacagcttg gttaaacgag
    20221 caaaaaattc tttggtaaaa tcattaatat tgaaattttt ctgccgcaac aattcttcat
    20281 aaaaattgtt ttgttgcaat gtaaagccat taatcacgat ggggtagagt ggagcagcca
    20341 aagcattaag ctctttggta tttaagctta aaatcaaatc tggatttaaa cttaaacttt
    20401 gcttgataaa ccaagctata tcagctcctg actggctgaa gtctgctggc cagttagcat
    20461 ctctaagcaa caatttatca gcaactaaaa tatttcttag ctcttcacta attgcttttt
    20521 caccataaac ccgttgatcc aaatccccaa cgtcatacac ttgccaatca agtagatggc
    20581 cgctttcaaa actcaggatc actacagaac ttaaaaaacc accgctcgac cttaactcat
    20641 tactgtcttg aattaaaata gcaatattgc ttctggatgg acttagtaaa tagtcgctta
    20701 gcttctctaa aaatgccgtc gttttttgtt ctgcacggag ctctttgctt aattgagctt
    20761 tataggcaat caagttttcg gcttgtgcat ccggcaaaat atccgggttt aattgttcta
    20821 gatattggct taattgttgt ttcacttgat aaagttcttt tttttgagtt aaaaaatcca
    20881 cccatgcggc ctcaggatta ctatccgtgg ccaaagtttc ttgatataga ttataagttc
    20941 gctccgttac agtttttgcc aattcttgcc ctcgcataat ttgtgcaaaa atctgccagt
    21001 aattgttggc ggtggataaa aagtcctcgc caagtatttt ttgataattt tgaagctgcc
    21061 actcaaaaaa agaatagttt ttactaattt cagccgtagt ttgcaaggag ttaccctgtt
    21121 caactccttg acgaaaaata gataagagat ttttttcaaa acttttttgg ctaataaaaa
    21181 aactcaaaaa taaaactaaa actagcgatc caacactggc caagagtact ccaaaataaa
    21241 aagacactcg tcgatattta cttttcttca caattttttt ggctccttga agatttttgt
    21301 caaagcgttt ttcatgtctt tccttttgtt gagtgcgaaa tatttgttgg agcttttgat
    21361 caatatcact tgatatatca acttttttat cttctttact tcgctttaat gtttcttctt
    21421 tggtagtttc cttaaccact ggtcgtaatt ctttgctctc taagctgttt gacacagctt
    21481 tttcgggctc ttcgaccacc ttttcctcaa ctctctgtag aggcggttcg acaataattt
    21541 tttgctcctt aagcgactgc aaatctttct tggttaacat taatgtctcg ttcagagaaa
    21601 ttggtaattg gcggattttt tgatctatga tctgagcgac tgagtcagtt gattgataaa
    21661 ttttgataaa gtccgcaagc tggaccggct cttcgttaga ttgcaaaaag agcttaacca
    21721 gactgaagtc ttccaaaaaa taacgactac acaaatcgcg cgcagcgatt aaaaaatctg
    21781 tacttgaggt ttttttgcct ctcaataaat atttctcacc agctttgccg tgaaaaaaaa
    21841 tctttctaaa gtttggccaa aaaccttctt ggctctgcca aaaacaggca gtttgaacat
    21901 caagtagtaa gcgctcttta aaaagactaa aaaacaaatt aaatttcaga ttaaaatcaa
    21961 actccaaatc aagataatcc tcaaataaac aaacttgagc taaaggaaaa ttttttaaaa
    22021 cactatttaa aaaccccttt tgtcttgacc aaaataaatc ataattattt aatttaatgt
    22081 tgatggggga gagtgcacta gagtaattta acagaaagcc aatgggtaaa acgcggcctt
    22141 cttctacaaa agctctctct aaagcactca aaacttggat aagaaactct ggttgtgcga
    22201 aaaaattata tttaaaaccg ccgactacta gaaatttata tacctctact tccttaatca
    22261 gttgatattg ttttagggct tgattggggt tggcaaagga gcttaataat aacggttggg
    22321 cctctagtcc acaattctta aaaaaaacca gcaattgttt ggtaaaaata ttttcttctt
    22381 caatcaacaa gaagaccgtt gtctgctctt caattaattc actggctaaa tacttttttt
    22441 gacccaacat cgctaaagaa cattgtaaat taaagccagg ctaattaaca agctcgatcc
    22501 attgcttgat tggctagttc gtcggctagt ttgttttttt ccctcagcac gtgtttaatt
    22561 tcaatcgagt agggaagagc acttaaattt ttccagattg cttcggctaa ttgttttaat
    22621 tcagtttttt taattttcca gcgtcggttg agttgctcaa caactagttt agaatccaaa
    22681 aaaaagttaa cactctgaat ttgtccagga ttttttaatt catagtgcat caaatattca
    22741 acagccatta aaaatgccaa atattctgct tcattgttgg tctttatccc taaaaatttt
    22801 gcttcccggt aaaactcttg gccgttttcg tcttccatat acaccccgcc gccagccatt
    22861 ccaggattgc ttctcgatcc gccatctgtg taaactttta accttgttgt catggtgtta
    22921 cagatcagtt attaccgcat aattgacgca taattcttgc aagctcaaga gtttttcttt
    22981 ggttcaataa gcaacactct tcccaaatca cgatcttctg aatgggtggt taaagattcc
    23041 aaaccaactt cgttagcaat tgtttcatga accaggtgtc tttcataaga atttaagtaa
    23101 cggaattcat agggtctgcc tgtttctaaa acgcgcttgg ctaaacttat ggctttttct
    23161 tttaagcgtt cttcttgacg caacttatat tgattaatat ccaaaacaat tcttttatca
    23221 ggatagtcat ctttaaaact taatttagtc aacaactcaa tagcttctaa agtttcacct
    23281 ttattaccaa tcaaaagagc cgtgtcttct tcatcggttt gaatattaat ctgcactcgc
    23341 tcgtcgctct cctcaatttt aatttcaatt tctccctcaa aaccgaggtg ttcgattaac
    23401 ttactaacat attcttcaat tttcatagtt tcccttaaga acgcttcttt ttgttgtgtt
    23461 tctttttttg acgattcttt ttgatttttt gttgaggttt aactttagta gccctggtat
    23521 ttttggcggg gagagcttca gtacttcctc ctttgtttgc taaaagttga gccaactcac
    23581 tagtttcttt ggtggcttcg ccttttttta gcgctttttt tagtagtggt tctttgacaa
    23641 ttttgtcttt tttttcttct tggagcaata gatttttccc ttgttcttca ggcaaaagtt
    23701 ttgctccaaa atagcctttt ttagcaatct gattattcca aaataccgca attccgccca
    23761 gtcccgatac aaaatattga gtgataatgc taaacaaagt gcccataatc caataaagta
    23821 ccaaacctga tggtagtctt aaagcaatta ttccagtcat gataggcatc ataaacatca
    23881 tttgttgctg catagttgaa gccatttcag caatatcctc ctctttttca ttagcctttt
    23941 tcagtaaatc gctttgagtt ttgttgggca caatgtctct agtttgagtc gctggggaaa
    24001 tcattaacga taaaacaaac tgacttagcg ccgctaaaat gggaaaaata aacttgttgt
    24061 ctggttggct gaggtcggcc cataaaaagc ggaagtttaa atcgctggcc tgattaaaca
    24121 ggcgaagaat cgcttgatac aaaacaatta agataagtag ttgaataatt tgtggcaaac
    24181 aaccggccag gggattgata ttatagcttt tgtacaattg ggcttgggcc aaggcataac
    24241 cctgtttatc atctttatat ttttccttga tcttttttaa ttctggctgc agttttttaa
    24301 ttttttgact agatttaatg ctgggtaggg taaggggcag aagcaacaaa cgaatgataa
    24361 tggtaaaggc aattaacgct aaacccacac cacccaaggc ttcatttaaa aatagtaaca
    24421 cttcgctaaa aaaattaata aaaaattcca taactattta ttaatggcag tgtagaaccc
    24481 gccatatccc tttcgttagc ccgataattg taccattttt tttaatttcc aggatggtat
    24541 attgaccaca gctgggttct tgaaaacaga ctggagaata accaaaaacg ctgtacatga
    24601 tgttgtggcg aatggtactt aataatttat aaagcttcat atttttttag tatctggtca
    24661 aaaaataatt ttaactcttt tgctatttct tcagacttag ctttattgtt tttgtaaaca
    24721 ataattaaat ccagctttag ctctaacaaa tttgttttag taataatttt atgatctcga
    24781 ttaatttgat tgattaactg ataaattaag cgtctgctgt gattgcgctc agcggctttg
    24841 ttaaaaagcc tggtcggagc caaggctgct actttcaggt gagattggtt ggtgcgataa
    24901 taaaagagca aattttcagt agaaaagcgc tcgctttttg taaaaagttg actattttca
    24961 gctacagata aattgaggcg gtgctttttt gccaacatta atttaatttt aacccaaagc
    25021 gcaaaaacta acctttttta atcacggtta gtttttttct tccctttgct cttcttttcg
    25081 ccaacacttt tcttccaccc aaactagaca ttcgattgag aaaaccatgc attcttaggc
    25141 gctttttttt ctttggattc caagttcttt gcataaggcg taattatagc ataagttatt
    25201 cgttaactct gaatggcata ctaatgtaaa aataattttt gttttctgga ctaataaatt
    25261 gagcaggctt taaactttca ttgatggaca tgatgatttt ttctgagtga acagcattaa
    25321 taaattcaat tagatagaca gcattgaaag caatttgaat ctcttcaatt tcgtcctttc
    25381 ccaaatccgt ttctaactca accagaatct cacttaaata ctctccataa cttggggaaa
    25441 tcgccttaat gactaagaaa gaacttttat cttgattttt aagtgataag cgaacaatgt
    25501 tagatgtttc tctggcaaag atttgtgctc tttttaattg atttaaaaat tcatcactat
    25561 cgatcgacac tgataattta aagctcggag gaactatttt ttgataagga ggaaaatctc
    25621 cttcaattaa tcttacaaaa aaatcaacac tgtctacttt aaacaaaagc tgttttagtt
    25681 catcggcaac cgtcacttcc acttcgtcag cctgactttg tttagcaatt ctcagtagct
    25741 cggtaattgc tttggccgga accaaaagtt cttgttcaaa agaaaacttt aaatcaggaa
    25801 aaaccgcgct ggctaaacgg aatccgtctg tgccaaccac tgttaggcct tgattagaaa
    25861 atttcattaa caaagcagtc agcaccggtc tggtttgatc attacttgag gcaaaaacca
    25921 ctaaattttg tatcttttcc aaatcagcag cttttagttt gaatttattg ccacttactt
    25981 caggaaattg gggatattca tcggcagatt g
//
LOCUS       DAOSEW010000002        15992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_3001_length_15992_cov_4.471670, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000002 DAOSEW010000000
VERSION     DAOSEW010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 15992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15992
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3001_length_15992_cov_4.471670"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..1303
                     /gene="epmA"
                     /locus_tag="PLJ02_00170"
     CDS             200..1303
                     /gene="epmA"
                     /locus_tag="PLJ02_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HOI04877.1"
                     /translation="MQKNWQRMRQDQFFSDQLLLREQVIKAIRKFFDQENFHEVETPL
                     LVRSPGTEPYLEVFATRLVDEQDKAQEAYLLTSPEYAMKKLLVAGLENIYQICKSFRN
                     HESFGDTHNPEFTILEWYRSQADYQKIMDDFARLMLFISQELQSYYQQQKGDFAQAIL
                     ARLQGDSVEYLGKKYYLTIPYPKMTVAEAFAKYLGINDQQLLDRDQLYHLAQDKGYPL
                     TAKDSWDDLFYQLFLNEIEPNLAKLAQPVIIYDYPLSQAALAKKKKNDPRFAERFEVY
                     LAGLELGNAFSELTDYREQEERLKAELVLRKKLKKTSYQLDQDFIEALKLGMPESGGI
                     AVGVDRLIMFFAGVNDINQVLFFPAQEIFQQVS"
     gene            1372..1938
                     /gene="efp"
                     /locus_tag="PLJ02_00175"
     CDS             1372..1938
                     /gene="efp"
                     /locus_tag="PLJ02_00175"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOI04878.1"
                     /translation="MSTVKAGNIRKGTYILYHHAPHYVLNVKFVSPGKGSAFSKVKMR
                     DMRSGSNVEFNFKSHDTVEELDIQSKEMQFLYKSGDEVIFMDPQSYEQVPVPAKLLGN
                     QVEMLTSEIKVYVIFYEDKALGVSLPPKVKLKVAKAEEAVAGDRQTAGKKPVVMETGL
                     VVQAPLFIKKGDVLSIDTSTGDYVSRVN"
     gene            1983..2056
                     /locus_tag="PLJ02_00180"
     tRNA            1983..2056
                     /locus_tag="PLJ02_00180"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2017..2019,aa:Lys,seq:ttt)
     gene            2107..3912
                     /locus_tag="PLJ02_00185"
     CDS             2107..3912
                     /locus_tag="PLJ02_00185"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HOI04879.1"
                     /translation="MPTEIRLLYIFSDLAYIAKLNPTKKAHEFAISDFYQLNGQFLDE
                     NSLIEKNIQKLVDKLEAGSYQLILPDFLFTNTMVNVEVKTEEEVKAYLKDQLLPDLKI
                     DTEDFYLDTNILSNYGGAFKVQLTALEKGIILPLVKALKNNQEVSIQAVAPLSFSIKS
                     IISLEPSVSILQINRELYLAQHYIGVDQCFNTSIDNANDFAETVKTLKGVEPSLQTLY
                     LLSDTLVDNKIKEVLAETLPVQQLAEPAEAGDKIPPYLKKIIEAGAKTMSIPDYLLPQ
                     FSIDQDYDKELAMQQEKEDEFDLVTNITKPVVIGEEETEKAEEESKLDMKDQELKKKE
                     EAKQDSQQLTDQEPTEPEVLPSPKKIDLAEVNKPNVPAKELRPKTKESVDKANEKEET
                     EINLAQFTNLAVDPSVLNKKAAKKSNDKESTMSNVKPVIKNSSEGSGVLKMIIIALLS
                     FVLTVALGVGIGYGVLQLTNKQEVPEVDISDEAAKVTEEPAPSPTPEPVVINKEDYQL
                     LVVNATAKAGYAGTIANTLEEAGFVDVVAANAKGEYEEGNYLLLNDLTAEQEASSQAL
                     LAEIEQITELDWQLADGIAVEDADGNYQAVLVLAQ"
     gene            3979..4464
                     /gene="greA"
                     /locus_tag="PLJ02_00190"
     CDS             3979..4464
                     /gene="greA"
                     /locus_tag="PLJ02_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOI04880.1"
                     /translation="MSKQVKDNQDKIILTEEGLADLKAELKDLKEVQMPKVVERIANA
                     RDQGDLSENSDYYSALDEQAMLNVRIDQIENVIDNAKVVKEQSGHLVTLGSKVQLQSE
                     SSNKKFTYSLVGQYESNPEDGKISIESPIGKSLLGKKKGDQVTVKTPAGELKYLILDV
                     S"
     gene            4556..6244
                     /gene="lysS"
                     /locus_tag="PLJ02_00195"
     CDS             4556..6244
                     /gene="lysS"
                     /locus_tag="PLJ02_00195"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOI04881.1"
                     /translation="MKNQNNEQSQHWLDQLVDEILLWQNQEGIKKLHVDDMKTPSGRV
                     HTGALRGVLLHDFVAKVLREHLSKLKQPALLKNLANTYVFNDMDPMDSLPKYLEEKNY
                     SQHMGKPLYQIPAPALAECGIDLSKISDQEKADLAAAKNFAEFYAKDFIHAFRKLGCS
                     QEIIWSHELYESGRMDELIRLSLDNVDKFRKIYKEVANYDLPDHWYPFQVICPHCGKI
                     GTTLVTDWDGKEVTFICREHGVKWAEGCGHQGKISPFAGNGKLLWKVDWPAHWSTLGV
                     SIEGAGKDHTSAGGSRDMAEAICREVFNLPIPFNIPYEWILIRGNKMSSSKGVGTSAR
                     EFTTLFPANVGRFLFLNKHYSQVIDFDPNTTAIPDLFDSYDQAARIYWGQEAGDQRLA
                     RAFVLAQIEEIPEPHFLARFRDLALWVQYPEKNVIEEAAKVKGSALNDLEISVINERL
                     SYVELWLSKYAPEEFQWRAKKDLPAAAQQLTQEQKTFLTELEKLIQSQNWQAPDLQQA
                     IFDLAKASLGTKQGFAAIYLAFLGKKAGPKAAWFLLNIDPQLRQERIEAINQLK"
     gene            6263..7516
                     /gene="serS"
                     /locus_tag="PLJ02_00200"
     CDS             6263..7516
                     /gene="serS"
                     /locus_tag="PLJ02_00200"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOI04882.1"
                     /translation="MLDINFIRQHPQEVKDACLHKQLDAAVVDQLLSVDEKKRALQVK
                     VDQMRQQSNQNAQEIKNQIAQGQQASQELIQKGKRIKDELKKIEPDLSNLENKFLELM
                     LAIPNIPSADTPIGSDETFNKVVREEGQIPHFTFPILAHQELMEKANWLDTKRAVKVA
                     GFRAYFLKNDGLRLERALLSYALDLMTNFGFEVMSVPTLVKPETMVGTGFFPWGKEDH
                     YYTQNGQILAGTAEVALTSYYQNELLREKDLPIKLCGISPCYRREIGTHGKDTKGIIR
                     VHQFNKVEQVVLTIADEEETRNWHTKMLKYSEQLLQDLGLPYRVVAMCTGDMGAGQRK
                     KYDLETWFPSQNTYRETHSASYFNDFQARRLNIRYQAKDGSIKYVYTLNNTVAASPRL
                     LAALVENYQQKDATIEIPKVLKKYF"
     gene            7546..7677
                     /locus_tag="PLJ02_00205"
     CDS             7546..7677
                     /locus_tag="PLJ02_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04883.1"
                     /translation="MIKEIIPEATEETVSTVRETRVAEAKHLLSVGGQVYRFLEMKC"
     gene            7682..10738
                     /gene="secA"
                     /locus_tag="PLJ02_00210"
     CDS             7682..10738
                     /gene="secA"
                     /locus_tag="PLJ02_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOI04884.1"
                     /translation="MFKFIKTLFDEEQKKLKQYQLLVDQINEFEPAMQAKSDTDLARQ
                     TDKFKEIIQGYREQVDPSLDPQSSAYSEAIQLKEEEALESILPEAYATVREVSRRIIG
                     MRHFDVQMLAGVAIHRGNITEQKTGEGKTLTVTAPLYLNALLGHGVHLVTVNDYLSEI
                     GVGWMGPIYHFLGLKTAVIIHDHAKLYDPQIDSEERGDERLEHFVEIRRQEAYLADIT
                     YGTNNEFGFDYLRDNMVTNLEQMVQRRDHTHHFAIVDEADSILIDEARTPLIISAPDT
                     EPTDKYYKFAKMVRSLTKEQDYKLDEKSKTATLTDLGVKRLEAKLGVNNLYEENFDTI
                     HHIEKALQAQSLYHKDKEYIVKDNQVIIVDEHTGRLMFGRRYSDGLHQAIEAKEGVTI
                     QQESRTLATISLQNYFRMYHKLSGMTGTALTESEEFNQIYHTDVLAIPTNVPVIRQDR
                     PDQIYKNTAAKYSAIIREVERIHATGQPILIGTRSIEHNQIISNFLRRKKIEHQVLNA
                     KNHEKEAFIIADAGKDGAITVATNIAGRGVDIVLGGAKPELKDFRKSRLTKKKSSVPV
                     AFKDLDLPSSINPANYQLKEYAQALKDWQQAHEKVVAVGGLAIIGTERHESRRIDNQL
                     RGRAGRQGDPGSSQFFVSLDDEIMRIFGGEQIAKLMTFLKIEESQAIENKMVSRAIES
                     AQTKVESFFFDQRKRLVDFDDVMNKHREIIYTRRRRLLEVATVSINKNNSDDRLANPI
                     VDSTGEQTTLKEQILDYVKQELSSAITSRLAVENSDVDVHDLIVKDFVKIIPFDEASQ
                     KRLVKKIANENDPAALLDQLMKIVQQTYELREDKIGEALMRELEKFVILSTIDEKWMD
                     HLDMIDDLRQGIWLRGDKNTVLSEYKREAFKMFEELIGNIQSTIAARIFRIQPMVQPR
                     AMPQNIQLGKADLQTTNLMQAVSQDPSQARGSKTQAKKPTGNNNDLARILAGASTSSQ
                     ATTNQPPSPPSPPVKAEKIGRNDPCPCGSGLKYKKCGLINAKEHRA"
     gene            10745..11623
                     /gene="mutM"
                     /locus_tag="PLJ02_00215"
     CDS             10745..11623
                     /gene="mutM"
                     /locus_tag="PLJ02_00215"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HOI04885.1"
                     /translation="MPELPEVESLRKNLSQVLLQKRIADIKLLRPKSFPEFSEKKQIL
                     IGQEFIEIKRRAKILDLVLSNSQHLLIHLKMTGQLIFVDKNGQRFGGGHPTADWINDL
                     PSQHTRVIFDLLDNENRISQLFFNDMRVFGWLKVIAASQLDQEFASYGPDVNDQQLTV
                     KYLLAKAKNRQTSIKQFIMDNQVLAGVGNIYANEALFRAKISPLRRANSLSKIEMTRL
                     LLEMRQIIEAAIILGGTTFDGRYVGINGLAGSFQDQLAVYGHENEVCPDCGHDFKIKR
                     VKLGGRSSFYCEHCQH"
     gene            complement(11620..12627)
                     /locus_tag="PLJ02_00220"
     CDS             complement(11620..12627)
                     /locus_tag="PLJ02_00220"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOI04886.1"
                     /translation="MTETKLLATRDGFATGLVKAAHHNPQIVALSADLSDSLRLAEFK
                     KLFPSRYIECGVAEQNLAGVAAGLSLAGKIPFATSFASFNPSRNFDQIRNIAYSKLNV
                     KIVGGHAGLVTGEDGAFHQSLEDLSLMIALPNMRVLSPADARQAELATLALAKTPGPG
                     YLRLSRLAVSDLEFLLKALDNKTDESANFALNQAQLLTKGQDISIVSTGILTHEAIRA
                     TLKLQQLGIRVELINVISLKPLDLDTIIQSCQKTQALLVLSEEQLGSGFNAYLAQHLI
                     TAVGLSMKKTIVTEFLAVDNSFGESGDAEQLLEKYGLNSQAILKKTLSLLKKKKNMLA
                     N"
     gene            complement(12624..13493)
                     /locus_tag="PLJ02_00225"
     CDS             complement(12624..13493)
                     /locus_tag="PLJ02_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013798835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOI04887.1"
                     /translation="MKITAAAQLHKIAFTLRQDILAMIYQAQSGHPAGSLGMVDLIFT
                     LYASGILRHDPQRPNWDKRDYFLLSNGHICPALYAVLAQQKYFAREELETFRQIDSRL
                     EGHPHFLMNGQTIDKKTVEGIEITAGSLGHGSSQAVGLAYGLQMDNKKNHVFCLLSDA
                     EQQEGQTWEAYMFATHHRLNNLTFIIDDNHIQISGKTQAVMSLGNLKLKLIAFGLQVL
                     EMDAHDFVDIQKKLQQAKDNKQHTVAIIAHSTPGKGVSFMENDYRWHGRAPNKQEFES
                     AMQELRIKKQQLS"
     gene            complement(13497..>15992)
                     /gene="dnaE"
                     /locus_tag="PLJ02_00230"
     CDS             complement(13497..>15992)
                     /gene="dnaE"
                     /locus_tag="PLJ02_00230"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOI04888.1"
                     /translation="KDRLDYEIKIINDKGYPAYFLITQDFVNWAKNNEIAVGPGRGSA
                     AGSLVSYCLNITTLNPLEHGLPFERFLNPERPTPPDIDIDFADIKRDDVLKYVANKYG
                     EDHVASVITFGRMEARVAVRDIGRVLGMPYEEPDRIAKLIPNEPGHRVSIKQAINTVP
                     ELGNYYQQSRFRKLLDLAMKVEGTIRQAGMHACAIIIADKPLSNYTPIQKDSRSGKTL
                     TQLDMYSLDCNVNDDAIGLLKFDLLGLRNLSIIQEALALIRKYKKENIKIDEISLEDE
                     KTFKLLASGDTTGVFQLESGGMRRIAKSLKPNKFSDITAMVALYRPGPMDLIPRFIEG
                     KHNPDIIEYPHPSLEPFLKETYGIMVYQEQILEIVNFMAGYSLGEADILRRAIGKKKK
                     KLLDKNKSRFIKQSVANGYKKATAERVWGFIEAFANYGFNKSHAASYAMIAYQTAWLK
                     ANYPVEYMTAVMSIESNSHSMNRDEKIAITIDNCRQMGIKILGPDINLSDKDFTIEKD
                     KESLDGLAIRFGLSAIKHVGAAAIENILSVRKQVKKFQSFTHFLQSTSNRKVNKKTIE
                     SLVKVGAMDRFANRSSMLENLEQIRQTAEQYNSEVDGQDNLFANIDNQSTKITDNFVE
                     LDEYPKPELLSFEKELLGVYLTSHPLADAMQMLNKRTSMRIGEIDPDVHQDQIFLFGG
                     VITKFKEVTTKSGQKMAFATLEDTTGKIEFLIFPKLYQKWIGQLGEDSIILLRAKVQN
                     RDKELKLIAEKISLPKTNLPQEISGDYQEIFVPRKTSKETLTKLGTLLKSNLGKTKIV
                     IIIPNGGKAEKINLPYQVDWNESLAKQVEELLK"
BASE COUNT         5097 a         2811 c         3283 g         4801 t
ORIGIN      
        1 cacttgggaa gttgatcgcc aagtagtgca gtttgacata ccctacctgt tatttacttc
       61 gattgtggcg gttttgtttt tattaagtaa aaataaattt gaacgtaatg aatctttatt
      121 aatgattgcc ctctatacca tttttatagt attaaagata tctggttttt aaccaattgc
      181 gtaatataat aacttcctaa tgcaaaaaaa ttggcaacgg atgcgtcaag atcaattctt
      241 tagtgaccag cttttgcttc gtgagcaagt gatcaaggca attagaaaat tttttgatca
      301 ggaaaatttc catgaagtag aaacaccact cttagttaga tctcctggca cagaaccgta
      361 cttagaagtt tttgccactc gtttggtcga cgagcaggat aaggctcagg aagcttatct
      421 gttaaccagc cccgaatatg cgatgaaaaa attattggta gctggtttag aaaatattta
      481 ccagatttgc aaaagttttc gcaatcatga gagttttggt gatacccata atccagaatt
      541 taccattttg gaatggtatc gctcccaagc cgattaccaa aaaataatgg atgattttgc
      601 acgattgatg ctatttatca gtcaagagtt gcaaagttat tatcaacagc aaaaggggga
      661 ttttgctcaa gcgattttag ctaggttgca aggcgatagt gttgaatatt tggggaaaaa
      721 atattattta actattcctt accccaaaat gacagtggcc gaagcttttg caaaatattt
      781 gggaattaat gatcaacagt tgttagatcg agatcaactt tatcacttag ctcaagacaa
      841 gggttatccc ctaactgcca aagacagttg ggatgatttg ttttatcaac tttttcttaa
      901 tgaaattgag ccaaacttag ccaaattagc tcagccggtg ataatttatg attatcccct
      961 gtctcaagca gcccttgcca agaaaaagaa aaatgatcct cgatttgcag agcggtttga
     1021 agtttatttg gccggtttgg aactgggtaa tgcttttagt gaattaactg attaccgcga
     1081 acaagaggag cgactcaagg cagaactagt tttgagaaag aaattaaaga aaacctctta
     1141 tcaactagat caggatttta ttgaagctct taaactaggt atgccagagt caggaggaat
     1201 tgcagtaggc gtggatcgtt taattatgtt ttttgctggg gtgaatgata ttaaccaagt
     1261 gctctttttc cctgctcagg aaattttcca acaagtaagt taaaattgta aggtttttag
     1321 cttgacttag cggtgaaatt gagctttaat aagccgatca agaaaactaa tatgtcaaca
     1381 gtcaaagcag gtaacatcag aaaagggact tacattttgt atcaccatgc cccccactat
     1441 gtgttaaacg ttaaatttgt ttcgcccggt aaaggttcag ccttttctaa agtcaaaatg
     1501 cgcgatatgc gcagtggttc taatgttgaa tttaatttca aatctcatga cactgttgag
     1561 gaattggata ttcaaagtaa agagatgcaa tttttataca aatctggaga tgaagtgatt
     1621 tttatggatc cgcaaagtta tgaacaggtg ccagtaccag ccaaattatt agggaatcaa
     1681 gtggagatgt tgacttcgga aattaaagtc tatgtgattt tttatgagga taaggctttg
     1741 ggagtgagct tgccacccaa ggttaaacta aaagtggcta aggctgaaga agcagtggct
     1801 ggggatcggc aaacggctgg taaaaagccg gtggtgatgg aaactggttt agtagttcaa
     1861 gcgcctttat ttattaagaa aggtgatgtt ttgtcgatcg atactagtac cggagattat
     1921 gtttctagag ttaattaaaa aaaattagta tataatagac agttgttcat taagcacaaa
     1981 atgggtccgt agctcagtcg gttagagcga cagcctttta agctgtgcgt cccgggttcg
     2041 agtcccggcg gactcacttt tttatttttc ttgcactcag cattctttat agttataatt
     2101 taggatatgc ccacagaaat acgtcttctc tatattttta gcgaccttgc ttatattgcc
     2161 aaattaaatc cgactaaaaa agctcatgaa tttgcgatta gcgactttta ccaactaaat
     2221 ggtcagtttc tcgacgaaaa tagcctcatt gaaaaaaata ttcagaaatt ggtggacaaa
     2281 ctcgaggctg gttcttatca gctgatcctt cctgattttt tgtttactaa caccatggtt
     2341 aatgtggaag taaaaactga ggaagaagtt aaagcatatt taaaagatca gttattacca
     2401 gatttaaaaa ttgatactga agacttttat ttagatacca atattttgag caattacggt
     2461 ggagcattta aggtgcaatt aaccgcttta gagaaaggga taattttacc cttagtcaaa
     2521 gcgttaaaaa ataatcaaga agtaagcatc caagccgttg ctccactaag ttttagcatt
     2581 aaatccatca ttagtctaga accctcagtt tcaattttac aaatcaatcg cgaactctac
     2641 ttagctcaac attatattgg tgttgatcag tgttttaata cctcaatcga taatgctaat
     2701 gactttgcgg aaacagtaaa aactctcaaa ggagttgaac ccagtttaca gactttatat
     2761 ctattatccg ataccttagt tgacaataaa attaaagaag ttttggccga aactttacca
     2821 gttcaacaat tggctgaacc agcagaagct ggcgataaaa ttccccccta cctcaaaaag
     2881 attattgaag ctggggctaa aacgatgtct attccagatt acttactacc acaatttagt
     2941 attgaccaag attatgataa agaattagct atgcaacaag aaaaagaaga tgagtttgat
     3001 ttagtaacta atattacaaa acctgtcgtc attggcgagg aagaaacaga aaaagctgaa
     3061 gaagaaagta aactagatat gaaagatcag gagttaaaga aaaaagaaga agctaaacaa
     3121 gatagtcaac aacttacgga tcaagaacca actgaaccag aagttttacc aagtcccaaa
     3181 aaaattgatt tagctgaagt gaataagccg aatgttccag caaaagaatt gcggcccaaa
     3241 acgaaagaga gtgtagataa agcaaatgaa aaagaagaaa cagaaattaa tttagcccaa
     3301 tttactaatt tggctgttga tccgtcggtg ctcaataaaa aagcagccaa aaaatctaac
     3361 gacaaggagt caaccatgtc taatgtcaaa ccggttatta agaattcctc agaaggtagt
     3421 ggtgttttaa aaatgattat tattgctttg ttaagttttg ttctgacagt cgctttaggt
     3481 gtgggtatcg gttatggagt tttacagtta accaataaac aagaagtgcc tgaagtagat
     3541 attagcgatg aagctgccaa ggttactgag gaacccgcac caagcccaac accagaaccg
     3601 gtggttatta ataaagaaga ctatcaattg ttagtcgtta atgcgaccgc taaagctggt
     3661 tatgcaggta ctattgccaa cactttagag gaagctggtt ttgtcgatgt ggttgcggcc
     3721 aatgctaaag gcgaatacga agaaggcaat tatttattgt tgaacgatct aacagcggaa
     3781 caagaagcta gcagtcaagc tttacttgct gaaattgagc aaataactga acttgattgg
     3841 caactggcag atggaatagc cgttgaagac gctgatggaa attatcaagc ggtgttggtt
     3901 ttagctcaat aatctcgacc aaaatcagcg attcggcact tgcaaagaat tagaaaaact
     3961 ttatacttat ggcctattat gtcaaaacaa gttaaagaca atcaagacaa gatcatttta
     4021 accgaagagg gtctagctga tttaaaagct gaacttaagg atttaaaaga ggtgcagatg
     4081 ccaaaggtgg tggaaagaat tgctaatgct cgagatcagg gagatctcag cgaaaattca
     4141 gactattatt cggctcttga tgaacaagct atgctcaatg tgagaattga tcagattgag
     4201 aatgtgattg ataacgcaaa agtagttaaa gaacagtctg gtcatttagt gactttaggt
     4261 tcaaaagtgc aattacaatc agaaagtagt aataaaaaat tcacttactc tttagttggt
     4321 caatatgagt caaatcccga agatggcaag atttctatcg agtcaccaat cgggaaatct
     4381 cttttgggca agaaaaaggg agatcaggtg acagttaaaa ctccggcggg agaattaaaa
     4441 tatttaattt tagacgttag ttaaaagcaa caaataaaaa aataaataac cccgccaaga
     4501 gcggggtttt tagtctagat aaagccaaat tttcaggcta aaatatgaga gatttatgaa
     4561 aaatcaaaat aacgaacaat cacagcattg gttggatcag ttagtggatg agattttatt
     4621 gtggcaaaac caagaaggga tcaagaaact tcatgttgat gatatgaaaa ctccctctgg
     4681 ccgagtgcac actggcgctc tcagaggtgt tttattacat gattttgttg ccaaggtgct
     4741 tcgagaacat ttatcgaagc tcaagcaacc ggcactcctt aagaatttag ccaatactta
     4801 tgtttttaat gatatggacc caatggatag tttacccaaa tatttagagg aaaaaaacta
     4861 ttcacaacat atgggtaaac cgctgtatca aattcctgct ccagctttag ctgagtgtgg
     4921 tatcgattta tcaaaaatca gtgatcagga aaaagccgat ttagcagctg ccaaaaactt
     4981 tgctgagttt tatgccaaag actttattca tgcctttcga aaattaggtt gcagtcaaga
     5041 gataatctgg agtcatgaat tatatgaaag tggtcgcatg gacgaattaa ttcgtttgtc
     5101 tttggataat gtcgataaat tccgcaaaat ttataaagaa gtagctaact atgatttgcc
     5161 ggatcattgg tatccttttc aagtcatttg tcctcactgt ggcaaaattg gtaccacttt
     5221 ggttactgat tgggacggta aagaagtcac ttttatctgt cgagaacacg gagtgaaatg
     5281 ggcagaaggg tgtggtcatc agggtaaaat tagccctttt gctggtaatg gtaaactact
     5341 ttggaaagta gattggccag cacactggtc cacactggga gttagtattg aaggggctgg
     5401 caaagaccat actagtgctg gtggttctcg agatatggcg gaagccatat gtcgggaagt
     5461 ttttaattta ccaattccct ttaacattcc ttacgagtgg attttaatcc gaggtaacaa
     5521 gatgagttct agtaagggag tgggcacttc tgcccgagag tttactactc tatttccagc
     5581 caatgtgggg cggtttttat ttctcaacaa acattatagc caagtcattg attttgatcc
     5641 aaacacgaca gcaattccag atttatttga tagttatgat caagctgccc gcatttattg
     5701 gggtcaagaa gcaggagatc aacgtttggc cagagctttc gttttagccc aaattgaaga
     5761 gattccagaa cctcattttc tagctcgttt ccgtgattta gcattgtggg tgcaatatcc
     5821 agagaaaaat gtgatcgaag aggcagccaa agtcaaagga tcagctctga acgatttgga
     5881 aatatcggtc attaatgagc gtttaagcta tgttgaatta tggttgagta aatatgctcc
     5941 agaagagttt cagtggcggg ctaaaaaaga tctaccagct gcagctcagc agcttactca
     6001 agaacaaaaa acttttttaa cagaattaga aaaacttatc cagagccaaa attggcaggc
     6061 cccagactta caacaggcaa tttttgattt agctaaagct agtctgggaa caaagcaggg
     6121 ttttgcggcc atttatttag cctttttggg caaaaaagct ggtccgaaag cggcttggtt
     6181 tttgctcaat attgaccctc aattgcgaca ggaaagaatc gaagctatta atcaattaaa
     6241 ataacaattt aaataaggaa ttatgctaga tatcaacttt attcgtcaac atcctcaaga
     6301 agtgaaagat gcttgtctcc acaagcaact cgatgctgca gtagtagatc aactattaag
     6361 tgttgatgaa aaaaaacggg cattgcaagt caaggttgat caaatgcgcc agcaaagtaa
     6421 tcaaaacgct caagagataa aaaatcagat agctcagggt cagcaagcca gtcaagaatt
     6481 aatccaaaag ggcaaaagaa ttaaagatga actcaaaaaa attgaaccag acttaagtaa
     6541 tttagaaaat aaatttttag agttaatgtt agctattccc aatatcccca gcgctgacac
     6601 acccattggt agtgacgaga cttttaataa agtagtcaga gaagaaggtc aaattcctca
     6661 ttttactttt ccgattttgg ctcatcaaga actaatggaa aaagctaatt ggttagatac
     6721 taaacgagcg gtcaaggtcg ctgggtttag agcctatttt ctcaaaaatg atggtttgcg
     6781 gttagaaaga gcgcttttaa gttatgctct tgatttaatg accaattttg gatttgaagt
     6841 gatgtcagta cctactttgg tgaagccaga aacgatggtc ggtacaggat ttttcccttg
     6901 gggcaaagaa gatcattatt acactcaaaa tggccagatt ttagctggta ctgctgaagt
     6961 ggcgctcaca tcatattatc aaaatgagtt acttagagaa aaagatttac caattaaatt
     7021 gtgcggaatt tcgccttgtt atcgtcgaga aatcggtacc catggtaagg ataccaaagg
     7081 gattattcgc gtccatcaat ttaataaagt ggaacaagtg gtgttaacga ttgctgatga
     7141 agaagaaacc agaaactggc ataccaaaat gttaaagtat tctgaacaat tgctacaaga
     7201 tttaggttta ccctatcgag tggtggccat gtgtactgga gatatggggg cgggacagcg
     7261 caaaaaatac gatctagaaa catggttccc cagtcaaaat acttatcgag aaacccacag
     7321 cgccagctat tttaatgatt ttcaagctcg tcgattaaat attcgctacc aggcaaaaga
     7381 tggcagtatc aaatatgttt atacgctgaa taatactgta gccgcctcgc ctcgcttgtt
     7441 agcggcactg gtagaaaact atcagcaaaa agatgctaca attgagattc caaaagtttt
     7501 aaaaaaatat ttctaattga ttaaagcttg tccagaaagg tgggaatgat aaaagaaata
     7561 attcctgaag cgacagaaga aaccgtatct acagtcagag aaactagagt tgccgaagct
     7621 aaacatctat taagcgttgg tggtcaagtt tatcgatttt tagaaatgaa gtgttagcat
     7681 tatgtttaaa tttattaaaa cgctttttga cgaagagcaa aaaaaactca agcaatatca
     7741 gttgttggtt gatcaaatca atgagtttga accagccatg caggctaaaa gcgataccga
     7801 tttggccagg caaactgata agtttaagga aattattcaa gggtatagag aacaggttga
     7861 ccctagtctt gatccgcaat cttcagccta cagtgaagca attcagttaa aagaggaaga
     7921 agccctcgag tctattttac ctgaagccta tgctacggta cgtgaggttt ctcgccgaat
     7981 aattggcatg cgtcattttg acgtgcaaat gttggctggt gtggccatcc atcgaggcaa
     8041 tattactgaa caaaaaaccg gtgagggtaa aactttaact gtgactgctc cactctacct
     8101 taatgccttg ttaggtcatg gcgtgcattt agtgacggtc aacgattact tgtcagaaat
     8161 tggcgtgggt tggatgggtc caatttatca ctttttggga ttgaaaacgg ccgtcattat
     8221 tcacgatcat gcgaaattat atgatcctca gattgatagc gaggaacgtg gcgatgaacg
     8281 tttagagcat tttgtggaaa ttcgccgaca agaagcttat ctagccgaca ttacctatgg
     8341 tactaataat gaatttggtt ttgattatct tcgcgataac atggtgacta atctagaaca
     8401 aatggtgcaa cggcgcgatc atactcatca ttttgctatt gttgatgagg ctgactcaat
     8461 tttgattgat gaagctcgga ctccgctcat tatttctgct ccagacacag agcccactga
     8521 caaatattat aaatttgcca aaatggtgcg ctctttgact aaagaacaag attacaagtt
     8581 ggatgaaaaa tccaaaacag ctactctaac cgatttaggt gtaaaaagac ttgaagctaa
     8641 attaggtgtc aacaatcttt atgaagaaaa tttcgacacc attcatcata ttgagaaagc
     8701 tcttcaagcc caatcccttt atcacaaaga caaggaatat attgtcaagg ataatcaagt
     8761 gattattgtc gacgaacaca ctggtcgctt aatgtttggt cgtcgttaca gtgatgggct
     8821 ccatcaagct attgaagcca aagaaggcgt aactattcag caagagtcac gcactttagc
     8881 tactatttct ttgcagaatt atttcagaat gtatcataaa ttgtctggta tgactggtac
     8941 agctttgact gaatctgaag agtttaatca aatttatcat actgatgttt tagcgattcc
     9001 caccaatgta cctgtcattc gccaagatcg tccagatcaa atttataaga atacagcagc
     9061 aaaatattca gccattatta gagaggtcga acgaattcat gccactggac agccaatttt
     9121 gatcggtact cgatcaattg aacataatca aatcatttct aactttttac gtcgcaaaaa
     9181 aattgaacat caagttttaa acgccaaaaa ccatgagaaa gaagcattta ttattgctga
     9241 tgctggtaaa gatggtgcca ttacggtggc cactaacatt gctggtcgtg gagtggatat
     9301 tgttttgggt ggtgctaagc cagaattaaa ggattttcgc aaaagccgtt taactaaaaa
     9361 gaaaagttca gtgcctgtag cctttaagga tttagatttg ccaagttcaa tcaatccggc
     9421 taattatcag ttaaaagaat atgctcaggc attaaaagat tggcagcaag ctcatgaaaa
     9481 agtcgtggca gtgggtggtt tagccattat tggtactgag cgtcatgaat cgcgtcgaat
     9541 agataatcag ttgcggggtc gagctggtcg ccaaggtgat ccaggtagtt ctcaattttt
     9601 tgtgtcactc gatgatgaaa tcatgcgcat ttttggtggc gaacaaattg ctaaattaat
     9661 gacttttctc aagattgaag aaagtcaagc cattgaaaac aaaatggtaa gccgagctat
     9721 tgaatcagcc caaactaaag ttgaatcatt cttttttgac caacgcaagc gcttggttga
     9781 ttttgatgat gtaatgaata agcatcgaga aatcatctat actagaagac gccgactgtt
     9841 agaagtagca acagttagta ttaataaaaa taatagtgat gatcggttag ctaatcccat
     9901 tgttgattca actggtgaac aaaccacact caaagaacaa attttagact atgttaaaca
     9961 agaattgagt tcagcaatta ctagtcgttt agcagtggaa aattctgatg tagatgttca
    10021 cgatttaatt gttaaagatt ttgtaaaaat tattcctttc gatgaagctt ctcagaaaag
    10081 attggttaaa aaaattgcta acgaaaatga tccagcagct ttactcgatc aattaatgaa
    10141 aattgtgcag caaacttatg aattaagaga agataaaatt ggtgaagcac tgatgcggga
    10201 attggaaaaa tttgtgattt tatcaaccat tgacgagaaa tggatggatc atctggatat
    10261 gatcgacgat ttacgacagg gaatttggtt gcggggtgat aaaaatacgg ttttatctga
    10321 atataaacga gaagctttta aaatgtttga agagctaatt ggcaatattc aaagcactat
    10381 tgctgccaga attttccgaa ttcaaccaat ggttcaacca agagctatgc cacaaaatat
    10441 tcaattaggt aaagctgatt tacaaacaac taatttgatg caagccgtga gtcaagaccc
    10501 cagccaagca agaggtagca agacacaagc taaaaaacca actggtaaca ataatgattt
    10561 ggccagaatt ttagctggag ccagtacaag ttcccaagcc acaactaatc aaccaccgtc
    10621 gcccccgagt ccgccagtta aagctgaaaa aattggtcgc aatgatcctt gcccgtgtgg
    10681 ctctggttta aagtacaaaa aatgtggctt gattaacgct aaagaacatc gggcttaatt
    10741 aactatgcca gagttaccag aagttgaatc tttacgaaaa aatttatccc aagtgctact
    10801 gcaaaaaaga attgcggata ttaaattact tcgacccaag agttttccag aatttagtga
    10861 aaaaaagcaa atattgatcg gtcaggaatt tattgagatt aagcgtcgag caaaaatctt
    10921 agatttagtg ttgagcaata gtcaacattt attgattcac ttgaaaatga cagggcaact
    10981 aatttttgtt gataaaaacg gacaaagatt tggtggcggt catcccaccg ctgattggat
    11041 taatgatttg ccaagtcagc acactcgagt aatttttgat ttattagata acgaaaatag
    11101 aattagccag ctttttttta atgacatgcg agtttttggt tggttgaaag tgattgctgc
    11161 tagtcaactt gatcaagaat ttgctagtta tggtcctgat gttaatgatc agcaactaac
    11221 agttaaatac ttgttggcaa aggccaaaaa tcgacaaact agtattaagc aatttattat
    11281 ggataatcaa gttttggctg gtgttggcaa tatttatgcg aatgaagcgc tgtttagagc
    11341 taaaatttcc cctttacgtc gagcgaatag tttaagcaaa atcgaaatga ctcgactgtt
    11401 attagaaatg cgccagatca ttgaagcggc tattattttg gggggtacca cctttgatgg
    11461 tcgttacgta ggaattaatg ggctggctgg gtcttttcaa gatcaactag cagtttatgg
    11521 tcatgaaaat gaggtttgcc cagattgtgg tcatgatttt aaaattaagc gagtgaaatt
    11581 gggtggtcga agtagtttct attgtgaaca ctgtcagcac taattagcta gcatgttttt
    11641 ctttttcttc aaaagtgata aggttttttt taggatagct tgactattca atccgtactt
    11701 ctctaataat tgttcagcat ctcctgattc gccaaaactg ttatcgactg ccaaaaattc
    11761 cgtgacaatt gtttttttca ttgataaacc aactgctgta attagatgtt gtgctaaata
    11821 tgcgttgaaa ccagagccta gttgttcttc acttaaaact aataaagctt gagttttttg
    11881 gcagctttgg ataatagtat cgaggtctaa gggtttaaga gaaataacat taattaattc
    11941 gactcgaata cccaattgtt gtagttttaa agttgcacga atcgcttcgt gagtcaggat
    12001 gccagtgctg acaatgctaa tatcttgacc cttagtcagt agttgagctt gattaagagc
    12061 aaaatttgct gattcatcag ttttattatc tagggctttt aataaaaatt ctaaatcgga
    12121 cacggccaac cggctcagtc gcaaatagcc tggcccggga gttttggcta gggctaaagt
    12181 agccaattcg gcttggcgag cgtctgcagg cgaaagcact cgcatattgg gtaaagcaat
    12241 cattaaactt aagtcttcta aagattggtg aaaagctccg tcttctccgg tcactaaacc
    12301 agcatgtcca ccaacgattt tgacattgag cttggaatag gcgatatttc taatttgatc
    12361 aaaatttcgt gagggattaa aagaggcaaa gctggtggca aagggaattt tgcccgccaa
    12421 actcaaacca gcagctactc cagctagatt ctgctctgcg acaccacact caatataacg
    12481 actgggaaat aattttttaa attcagctaa gcgcagtgaa tcacttaagt cagcagataa
    12541 agccacaatt tgcgggttgt ggtgagcggc tttcaccagg cctgtggcaa aaccgtctct
    12601 ggtagctaat aattttgttt cagtcatgat aattgctgtt ttttgattct taattcttgc
    12661 atggcacttt caaattcttg tttattggga gctcggccgt gccaacgata gtcattttcc
    12721 atgaaagaca cgcctttgcc gggcgtacta tgagcaataa tggcgacagt gtgctgcttg
    12781 ttatcttttg cttgttgcag ttttttttga atatcgacaa aatcatgggc atccatttcc
    12841 aacacctgca ggccaaaagc aattaatttt aatttcagat tgcccagtga catcactgct
    12901 tgagttttac cagaaatttg aatgtgatta tcatcaataa taaaagttag attgtttaat
    12961 cggtgatgag tggcgaacat ataagcttcc caagtttgtc cttcttgttg ttccgcgtca
    13021 ctaagaagac aaaaaacatg attctttttg ttatccatct gtagtccata agctaaaccg
    13081 actgcttgtg agctaccgtg tcccaaagat ccagcagtaa tctcaatccc ctcgacggtt
    13141 tttttatcaa tggtttgccc attcattaga aaatgagggt ggccctctaa gcgactgtcg
    13201 atttgtctaa atgtttctaa ctcttctcga gcgaaatatt tttgttgagc taaaaccgca
    13261 taaagtgctg gacaaatatg cccattggac aataaaaaat aatctcgttt gtcccagttt
    13321 ggtcgttgtg gatcatgacg caggatgcca ctagcgtaga gggtgaatat cagatcgacc
    13381 atacctaaac taccagctgg atgaccgctt tgagcttgat aaatcatcgc taaaatatct
    13441 tgacgaagag taaaagcaat tttgtgtaat tgggcagcag cggtgatttt cattgcttat
    13501 tttagtaatt cttcaacttg tttggctagt gattcattcc aatcaacttg atatggtaaa
    13561 ttaatttttt cggctttgcc accattggga ataataataa cgattttggt tttgcctaaa
    13621 ttagatttta ataatgtgcc gagctttgtt aacgtttctt tactggtttt tcttggcaca
    13681 aagatttctt gataatcacc tgatatttct tgtggcaaat tagtttttgg taaggaaatt
    13741 ttttcggcaa tgagtttcag ttctttatca cgattctgta ccttagctct tagtaagata
    13801 atactatcct cacccagttg cccaatccat ttttgataaa gtttaggaaa gattaaaaat
    13861 tcgattttac cagtggtgtc ctccagagta gcaaaagcca tcttttgacc acttttggtt
    13921 gtcacttctt taaattttgt aatcactcca ccaaaaagga aaatttgatc ttggtgtacg
    13981 tctgggtcaa tttccccaat tctcatgctg gttcttttgt tgagcatttg catagcatca
    14041 gctagggggt ggctggttaa atagacacct aataactctt tttcaaatga taacaattct
    14101 ggtttgggat attcatctag ttctacgaaa ttgtccgtaa ttttggtact ttgattgtca
    14161 atattagcaa ataaattatc ttgtccatct acttctgaat tatattgttc agcagtttgg
    14221 cgaatctgct ctaagttttc tagcattgat gagcgattgg caaaacgatc catcgctccc
    14281 accttaacca agctctcaat agttttttta ttaactttgc gattactggt gctttgtaag
    14341 aaatgggtaa aagattgaaa tttctttact tgttttctaa cgcttaaaat attttcaatg
    14401 gcagccgctc caacgtgttt gatggcactt aaaccaaaac gaatggctaa accatcaagt
    14461 gattctttat ctttttcaat agtaaaatct ttatcagaga gattgatatc tggtcctaaa
    14521 attttgatgc ccatctgtcg gcaattatca atagtaatag caattttttc atctctattc
    14581 attgagtgcg aatttgactc aatgctcatc actgcagtca tgtactcgac tggataattg
    14641 gcttttaacc aggcagtttg ataagcaatc atggcataac tggcagcatg agatttatta
    14701 aaaccatagt tagcgaaagc ttcaataaaa ccccaaactc gttcggcagt ggctttttta
    14761 taaccattag ctactgattg tttaatgaag cggcttttat ttttgtctag tagtttcttt
    14821 ttctttttac cgatagcacg gcgtaaaata tcggcctcgc ccagactgta accagccata
    14881 aaattgacaa tttctagaat ttgctcttgg taaaccatga tgccataggt ttcttttaga
    14941 aatggttcca gtgagggatg aggatactca atgatatcgg gattgtgttt gccttcaata
    15001 aagcggggaa tcaggtccat aggaccagga cgatacagcg ccaccatagc ggtaatatct
    15061 gagaacttat tgggttttag acttttagca atccgcctca ttccaccaga ttccaactga
    15121 aagacgccag tcgtatcgcc actagctaag agtttaaagg ttttttcatc ctcgagcgaa
    15181 atttcatcga ttttaatatt ttcttttttg tattttctaa ttaaagctaa ggcttcctgg
    15241 atgatagata aattacgtaa acctaataag tcaaatttta acaagccaat ggcatcatca
    15301 ttaacgttac aatcgagcga gtacatgtct agttgagtga gagttttacc tgaacgggaa
    15361 tctttttgaa ttggggtgta attggacaac ggtttgtcag cgataataat ggcgcaagca
    15421 tgcatgccag cttggcggat cgtaccttca actttcattg ccaaatcaag taattttcta
    15481 aacctacttt gttgataata attacccagt tcaggtacag tgttaatagc ttgcttaatt
    15541 gacacccggt gtcctggctc attaggaatc aatttagcta ttcggtcagg ttcttcataa
    15601 ggcataccta ggacgcgacc aatatcacgc accgctactc ttgcctccat ccggccaaag
    15661 gtaatgacag atgcgacgtg gtcttcacca tatttgttag ccacgtattt taaaacatca
    15721 tcgcgcttga tgtcagcgaa atcaatgtcg atatcaggag gtgtaggtct ttctgggttc
    15781 aagaaccgtt caaaaggtaa gccatgttcc aagggattaa gcgtggtaat gtttagacaa
    15841 taggagacca aagaaccggc ggcactccca cggccagggc caacggctat ttcattattt
    15901 ttagcccagt ttacgaaatc ttgggtaatc aaaaaatatg ccgggtagcc tttatcatta
    15961 atgatcttga tttcataatc taaacgatct tt
//
LOCUS       DAOSEW010000003        14984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_3271_length_14984_cov_4.287762, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000003 DAOSEW010000000
VERSION     DAOSEW010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 14984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14984
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3271_length_14984_cov_4.287762"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="PLJ02_00235"
     CDS             complement(<1..966)
                     /locus_tag="PLJ02_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOI04889.1"
                     /translation="MKKNIVFIVLVLLAFAGGVYLSNTGQIEKILPNSLGEKAGTEDL
                     TTDHRITSFSDDQLSISEVVEAVGPSVVTISAERNVLRPGSFSFSPFGFGSLFEQFYQ
                     SPSEEDFETIEQDIGTGFVVLAKESNQLFVITNRHVVSDFNLNYLVYDYQDQVYEVTD
                     IYRDPVNDLAILNVKDLNLPALTLGDSDQIKIGESVIAIGTALGQFRNTVTSGIISGV
                     GRGITASNVFGGQQENLDNIIQTDAAINPGNSGGPLINARGEVIGVNVATSAGADNIG
                     FALPINLVKNSLANFEATGKFDRAVLGVAYTLISEQAAIVNEVPQG"
     gene            complement(1000..1416)
                     /locus_tag="PLJ02_00240"
     CDS             complement(1000..1416)
                     /locus_tag="PLJ02_00240"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HOI04890.1"
                     /translation="MNLVRWRPYQSLLDTWPDVWEDDFLTDNSQRQHLNIYEKDDEVV
                     VEANVAGVRADQIDLSFDKGILWIKAEAEKEEGKKDKKHYSKTSWSYSYKVAVPGQIN
                     QQSEPKAELSKGILKIRFKKQAIAKPKRLKITEKND"
     gene            1575..2054
                     /locus_tag="PLJ02_00245"
     CDS             1575..2054
                     /locus_tag="PLJ02_00245"
                     /inference="COORDINATES: protein motif:HMM:NF025833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HOI04891.1"
                     /translation="MVKRNSAKILSIIVGIIGGLVVIVLILASLFPDLNLPTFQSLLR
                     DENYEQSDQEQIENTEELTQESNNPIAQEAKVISLTASEDNTRAWDLLVSNHQIIYQE
                     YDFGIFLEGIDGLKGDQENFWAIYVNGEKSEVGISDIILNQGDLIEFKYEAIETAAN"
     gene            2026..2514
                     /locus_tag="PLJ02_00250"
     CDS             2026..2514
                     /locus_tag="PLJ02_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04892.1"
                     /translation="MKQLKQQLISALGILVVRIGKILPANFSPLGSFGFFSGNPLLFF
                     LSILIFDYFFGGFYLGQSWVYLAFSIYPLLGYLSRGKIKRQVFLIPLASLLFFLISNF
                     GVWLNWYPRTTEGLVHCYLLALPFYGRTLMGDLSFGYGYLLLRQLVKKYQHHFLVPDK
                     IN"
     gene            complement(2531..2881)
                     /locus_tag="PLJ02_00255"
     CDS             complement(2531..2881)
                     /locus_tag="PLJ02_00255"
                     /inference="COORDINATES: protein motif:HMM:NF013869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HOI04893.1"
                     /translation="MTKKLSEPTSKTMIKKPALDFLDFIREQGVVGLAIGFILGGSIR
                     SVVDSIVNDLINPLLGLLLGKVNFSQAVWQIGSAQIAWGNFIATLINFTIVAAVVFFG
                     VKGLGLEKLDKKKK"
     gene            2950..3939
                     /locus_tag="PLJ02_00260"
     CDS             2950..3939
                     /locus_tag="PLJ02_00260"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04894.1"
                     /translation="MSNEVTEKSLVKKVAILYSDAKREYFPTEQLYITEAEVKGRAEL
                     IANILRAKGFIVELFPGDARLHEALLKFSPDKVINLVDSVYGKEYLAGTIPATLELLQ
                     IPYTGSGMLAISINANKYLTKSLLEQYGVTTPKYQLIKHSSDEIDEELDFPLFIKLNE
                     NHGSLEVSDGSVGFDEKMAKNQIDYLYKTYQQPILLEEFIAGREITVVLVDGGNTKIY
                     AAEKIFHRSSDDPFKKIVTFDDNWVSKEETITYLKYELPDPVKRVAKTVFNILKIEDY
                     AKLDFRLDEAGRHYLIDVNVNPALGPSESCAIGSILELYGVSFYTLLDRLINN"
     gene            3943..4686
                     /locus_tag="PLJ02_00265"
     CDS             3943..4686
                     /locus_tag="PLJ02_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOI04895.1"
                     /translation="MSKNNFYHLKKVEDFCLRDNRWVSLDWRLKLNNLAQLKKELSVA
                     KQYAKKRFVNKIEVLLPITIPQELAELLYSENFKPALVELKFDLKKFTQASYNIANLN
                     FSSQIKNKSALKDLLIEQADFHHQNLPDYYLAAREINWFFYLRQFLNDATKENGLLLL
                     IEDKGDLSGLVLGEYLGKTAFIWELVVCQKKRHFHLGSYLLNQYLAKLKDLGIARVYL
                     ETMSNSYARSWYQHQGFTEVSQSWFSRLK"
     gene            complement(4724..5311)
                     /locus_tag="PLJ02_00270"
     CDS             complement(4724..5311)
                     /locus_tag="PLJ02_00270"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOI04896.1"
                     /translation="MLIHTCCADCFLKFYQALSSEEQKQSQIFFYNPNLYPREEFNAR
                     LQAVKKVVKSLDPDIKIFVENYLPQDYFHALATNRSINELREKNQSQQIFTVIPQSRR
                     CPNCWQLRLRALFEKAKQEHISLVSSTLLSSNYQDLEQIKKIVERLSAEYQIDFYFPK
                     LTKLDRNRVNKGFYKQNYCGCLYSLIEKSREKYQI"
     gene            5365..7182
                     /gene="dnaG"
                     /locus_tag="PLJ02_00275"
     CDS             5365..7182
                     /gene="dnaG"
                     /locus_tag="PLJ02_00275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOI04897.1"
                     /translation="MSQIQEVKDANNIVEIVGEKIDLRPSGSSFKARCPFHSEKTPSF
                     FVHPEMQRYRCFGCGATGDVLDFLQNYDGMTFYEALKYLADRASITLKDFNRTSEDEE
                     REQLLGILNLAKEYYHYLLTQHEAGKPALAYLKERGTNSQSIKLFNLGYSLSSWDGLI
                     KYLHQKKKYPLELIAKTGLIIKGKGDRYYDRFRGRVMFPLKNHRGQVVGFSGRLLTAQ
                     AKEAKYINSPETSLYHKSKLLYGYSELFREIRKANKVVVVEGEFDVISSAQAHVNNVV
                     AIKGSALTADQVDLLARVSDSVILAFDADEAGINACKRAITLMKDKDIDLRVIDFPAL
                     HQLATAKDLKDPDDFARQEPKLWRQAVDQAISIYSFLLNTTLAHYDAETPDGKRKIID
                     ELAPIINGIDHLVEKEFYLQKLSEKLNVKLGLIKEDIARVAEKKNKKTTVVKVKPPIE
                     PNKQKAKSHQEIMEEYLLFLLFHLEKDAFEEGVNNIIGLKWQTPGLSNIVDILSRELK
                     NFDLEKFSKRLAEDLKAILMEILLNPEFEKNIKNTTPKREWQRVLEQVKKNIVHSEIE
                     AINQEINELDKKNQRTKDEEQRLDQLLEQIVQMQRQLKS"
     gene            7237..7500
                     /locus_tag="PLJ02_00280"
     CDS             7237..7500
                     /locus_tag="PLJ02_00280"
                     /inference="COORDINATES: protein motif:HMM:NF014624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HOI04898.1"
                     /translation="MAFIRNKISLAKKDQVITQLKRIRGQIDGLIRMYEDERPCIEIA
                     QQISAARNSLSSVARNVLNNAADNCIKNKDQKQLDLIVKELLK"
     gene            7522..7860
                     /gene="trxA"
                     /locus_tag="PLJ02_00285"
     CDS             7522..7860
                     /gene="trxA"
                     /locus_tag="PLJ02_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOI04899.1"
                     /translation="MSAQHLDSSNFQSTLDNATTPVMVDFYADWCGPCQMAAPIIDKL
                     ADDYQDKMMIVKLDVDEARDIASEFNVMNIPTVIIFKKGDDNKMVEFDRKIGFPGETG
                     YQKMIEEALA"
     gene            7930..9006
                     /locus_tag="PLJ02_00290"
     CDS             7930..9006
                     /locus_tag="PLJ02_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOI04900.1"
                     /translation="MKNLKVAVIGAGPAGFTAGIYLGRALLKPVLFSGINLGGQLMFT
                     RDIENFPGFPDGMRGPQFMLSLQKQAVKFDAEICHEQITAVDFSQRPFRLWNSLPADM
                     EFADWQHLAKAELLKITESIKKENEAAYSADSVLISTGAEHMKLGVPGEEAYFGKGVG
                     FCAVCDGAFYRDKKVFVVGGGDTALSDALTLSKFTDQVFLIHRREQFRACKILQQRVA
                     ADEHIKIFWNTEIKEIVGDEQRVTALKLLRDGKEEQVAADGIFIAIGYQPNSALFSNQ
                     VKLDDHSYILTRQFGSDQALAELIQEPTPTTQDYFPTMTSVPGVFAAGDVTDSHYRQA
                     VVAAGSGAMAALDIERWLELESNK"
     gene            complement(9019..9894)
                     /locus_tag="PLJ02_00295"
     CDS             complement(9019..9894)
                     /locus_tag="PLJ02_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOI04901.1"
                     /translation="MKIRKAVITSAGFGTRFLPISKTIQKEMLPIINRPLIDYVVEDC
                     VKAGITDIVFVVSEHNYQVLHYYRENQRLHQYLKDNNKLELYDLIKDLHTKANFHFIK
                     QSDADDYGTAVPVQLAKEYLQDEEAFLVLMGDDFIYRHNGTSEVAAMMQTYEQSGAAA
                     LVTCIEKAESDLPRYGVAKLSEENNFRYLQALVEKPKPGTAPSNLANISKYILPQSVF
                     DLIAVQQPNPASGELYITDTISQLALTEKVVIHQPQGEYLDGGYPLGWLIANLTVAKD
                     HPELWAEIKRFVNQE"
     gene            9936..10550
                     /locus_tag="PLJ02_00300"
     CDS             9936..10550
                     /locus_tag="PLJ02_00300"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOI04902.1"
                     /translation="MQFILASQSPQRARLLATLPYRFKIYPANINESAIKFKNPRDKA
                     KKIALAKANAVAKNVAGEAIIVAADTFCLCRGKILEKPKSKAEARKMLELQSQQELKA
                     ITGYAFIYQNSSKKIIENSSVVTQVRFRHLSSPEIENYISHQPVLTWSAAFSPAYDAG
                     MALLAWSKGNLTAFTHGLPIDLLVEFINQHLNLGVENKHHTDKP"
     gene            complement(10512..11477)
                     /locus_tag="PLJ02_00305"
     CDS             complement(10512..11477)
                     /locus_tag="PLJ02_00305"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA prenyltransferase family protein"
                     /protein_id="HOI04903.1"
                     /translation="MKSFFNSQLFLLLKTARPKQWLKNFALYAALVFSGFFFYKPEDG
                     PSYFYLVSYAFIIFCLLTSSTYILNDLFDIEADRKHPFKKNRPLASGKLSIPTAIFAI
                     AFCLALVFWLSLPLTLVFKLLIFAYLILQILYSLYFKQIAILDIISIAAGFLIRVYAG
                     AVVVNLHMSVWFLLTVISMSLFLAVGKRQSERTLMTEHKLSFVRKSLNTYSARLLDQY
                     TSIFATSTWLTYALFAFQSSPGDGLNLLYERFPNLVINLPRTLQSQKLLMLTLPLVIF
                     GIMRYLQLVYEENRGEAPADVLLKDKTLLVTVFLWFISVMFVFYA"
     gene            complement(11474..12517)
                     /locus_tag="PLJ02_00310"
     CDS             complement(11474..12517)
                     /locus_tag="PLJ02_00310"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOI04904.1"
                     /translation="MWPLIISFLISFSLGPLVINFYQKHHWVDDPKKNHHVKVTHTKP
                     VPRGGGILIFIAVFLSSIIFLHFDKYLIAIFLGAFILTIVGIIDDIFNISPYWRLLTG
                     LLATLIVVGSGIGIAYVSNPLGPGVIHLDQPQLHFVLFGEERNIWLLSSAFAILFIMW
                     NMNIINWSKGVDGQLPGFVMVACLFIAVLANSFQDAPSQFNVAQLALIVSGAFAGLLY
                     WNFYPQKIMPGYGAGSLAGYFLSVLAILSGAKVATTLMVLAIPTADGIFTIVRRILAG
                     KSPFWGDRGHLHHKLMDNFGWDPRRISIFYILTSFFMGFLSLFLNTTGKIISIVLVSL
                     FVFAFLIYNKLRK"
     gene            complement(12521..12904)
                     /locus_tag="PLJ02_00315"
     CDS             complement(12521..12904)
                     /locus_tag="PLJ02_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04905.1"
                     /translation="MDVSFRDSASRKKEFKYLTFLPKNFRQLALYALIANISTLALSL
                     IFFSSLQKTIPLFYSLPDDQQLVNRELIFILPGMSTLINALHFFIIKKFKTAHPTILQ
                     VFLLSTVAIQILILAILLRLLIILN"
     gene            complement(12936..14522)
                     /locus_tag="PLJ02_00320"
     CDS             complement(12936..14522)
                     /locus_tag="PLJ02_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04906.1"
                     /translation="MSFKKTIKKIDLFLDQQTALFINLFLLVLLRIPNFFEPYWYGDE
                     AIYLTVGNALNHGGKLYTTIIDHKTPLIYQFARVPNQFYFRLLNLVWMVVATTFFWLF
                     AKKLFKKELPSFIATLIMVLLTSLPWLEGNIPNGELFVLGFVIVGAFLFSQTQIWQNF
                     FSKKISWPKEKKKESLLLFASGVLFSLGILTKVPALLDLVPFLSIFALLLLADALKVN
                     KTFKDWQKTCGQLLWRLLYFSAGIVVPILISIIYYQAIGSGRDYLEYGLLYNLRYSQS
                     WNPSFNSAFMDFSFSLLGKTLYFFSFLILIALNSTELNKRFQFLSIWFAATLYATLLS
                     NRPYPHYFIQMVPPFALLLVEMGSHFQKKLVGVKKHYKSLVTGLALISLTIFVMLTLG
                     FKPYSTSKYYSQFWRMATGQIAKAEYDYSFNNLTRDNQKLATLIDELALEKIFIWGDN
                     AMLYAQTKTVPTSRFTVAFHIKDFADYEGTLAQIKEEEPKLIVVMKNDQHTFPQLNTY
                     LKENYLVNSQFDHVNLFLKK"
     gene            complement(14573..>14984)
                     /locus_tag="PLJ02_00325"
     CDS             complement(14573..>14984)
                     /locus_tag="PLJ02_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04907.1"
                     /translation="PFIDKTLRVLHVIEDHFLWPVNWFAVTVSAMLPPLLNQQFNRTI
                     IGKTLPQVTSTLLTMSMISMLSIFIIDAKNRPPRPKKNLWSYISQPLEFLLIPVVGFF
                     FSALPGMDAHTRLMFGKYLEYRVTEKVANPQTNK"
BASE COUNT         4986 a         2513 c         2903 g         4582 t
ORIGIN      
        1 tccttgagga acttcattga cgatggctgc ttgttcggaa attaaagtat aggcaacccc
       61 cagtacagcc cgatcaaatt tacctgttgc ttcaaaattg gccaaggaat ttttgacaag
      121 attgattggt agggcaaagc caatattatc tgccccggca gaagttgcta cgttaactcc
      181 gattacttct ccacgagcat taattaacgg cccaccagaa ttgcctgggt taatcgcggc
      241 gtcggtttgg atgatattat ctaaattttc ttgttgacca ccaaacacgt tgctggcagt
      301 gatgcctctc cccacaccag aaatgatgcc actggtgacc gtattacgaa attgaccaag
      361 ggcagtccca attgcaatca ctgattcacc aatttttatt tgatcagaat cgcctaaggt
      421 taaagcaggt aaattcaaat cttttacgtt taaaatggcc aagtcattga cgggatcgcg
      481 ataaatgtca gtcacttcat aaacttgatc ttgataatca taaaccaagt aatttaaatt
      541 aaaatcactg acaacatggc gattagtaat gacaaataat tgattgcttt ctttggctaa
      601 aacgacaaaa ccagtgccaa tgtcttgctc tatggtttca aaatcttctt cactaggaga
      661 ttgatagaat tgctcaaata aagagccaaa accaaatggt gaaaaactaa aactaccagg
      721 tcgaagcaca tttctttcgg cactgatggt gactactgac ggcccaacag cttccaccac
      781 ttcactaata ctcagctgat cgtcgctaaa actagtgatt cggtgatcag ttgtcaagtc
      841 ttcagtgccc gccttttccc caaggctatt tggcagaatt ttttctatct gaccagtatt
      901 gctcagataa acaccgccag caaaggcgag caggactaaa acgataaaaa caatattttt
      961 tttcatagtt ttggctcttt aattaattaa ttttactaat taatcattct tttctgtaat
     1021 tttgagtctt tttggtttag caatggcttg tttcttaaat ctaattttaa gaataccttt
     1081 gctcaactca gccttgggtt cgctttgctg attaatttgt ccaggaactg ctaccttata
     1141 ggaatagctc cagctagttt tggaataatg ctttttatcc tttttacctt cttctttttc
     1201 agcctcagcc ttgatccaaa gaatgccttt gtcaaagctc aaatcaattt gatcagctcg
     1261 aacaccggcg acattagcct caacaactac ctcatcatcc ttttcataaa tgttaagatg
     1321 ttgacgctga ctgttgtcgg ttaaaaaatc atcttcccaa acgtctggcc aagtatctaa
     1381 taaagattgg tagggacgcc atcgaactag attcatagtc actcctttct ttaactaact
     1441 aataaactgt ctttagcagt cacacgagtt gactgctaag tataatttaa caattgaata
     1501 aaaaattgtc aagtgttttt ttaatgttta tattcagttc ataatttttt tcaactgtgt
     1561 tacaattttt ttctatggtc aaaagaaact cagcaaaaat tttatcaatt atcgttggta
     1621 ttattggtgg tctagttgtc attgttttaa ttttggcatc actctttccc gacttaaatt
     1681 tacccacttt tcagtcttta ttgagggatg aaaattatga gcaaagtgat caagaacaaa
     1741 tagaaaacac tgaagaacta actcaagaat caaacaaccc aattgcccaa gaggcaaaag
     1801 ttattagctt gacggctagt gaggataata ccagggcttg ggatttgttg gttagtaatc
     1861 atcaaatcat ttatcaagaa tacgattttg ggattttttt agaaggcatt gatggtctca
     1921 aaggagacca ggaaaatttt tgggcgatct atgttaatgg agaaaaaagc gaagtaggta
     1981 tttctgatat tattcttaat cagggagatt taattgaatt taaatatgaa gcaattgaaa
     2041 cagcagctaa ttagtgcgct gggcattctc gtcgttagaa ttggtaaaat cttacctgct
     2101 aattttagtc ctttaggaag ttttggtttt ttttccggca atccacttct ctttttttta
     2161 tcaattttaa tttttgatta tttttttggt ggtttttatt taggtcagtc ttgggtttat
     2221 ttagccttta gcatctaccc gctgttgggt tatttaagtc ggggcaaaat taagcgacaa
     2281 gtctttttga tcccgctggc ttctttattg tttttcttaa tttctaattt tggcgtgtgg
     2341 ttaaattggt atccaagaac aactgaaggt ttagtacatt gttatttatt agctttaccg
     2401 ttttatggga gaactttgat gggggatttg agttttggct atggctattt actgttgaga
     2461 caattggtta aaaaatacca gcaccacttt ttagtgccag acaaaattaa ctaattactt
     2521 ttttagctaa ttattttttc tttttgtcta atttttccaa gcccaaacct ttgacgccaa
     2581 aaaaaactac tgccgccaca atggtaaaat taattaaggt cgcaataaaa ttaccccaag
     2641 ctatttgggc cgaaccaatt tgccaaactg cttgagaaaa attaacttta ccgagtagca
     2701 aacctagcag aggattaatt aagtcattga caattgaatc gaccactgag cggattgatc
     2761 cacctaaaat aaaaccgatg gccaagccaa caacgccttg ttcccgaata aaatctaaga
     2821 agtctaacgc tggttttttg atcatggtct tggaggtggg ctcgcttagt tttttagtca
     2881 tgggcgcatt atagcaaaaa ttattcttat tttcttattc cactattgag ctataattaa
     2941 aagttatcga tgtcaaatga agtaactgaa aagtcccttg ttaaaaaagt ggcaatttta
     3001 tattcggacg ctaagcgaga atactttcct actgaacagc tctacattac ggaagctgaa
     3061 gtcaaggggc gagcagagct gattgctaat attttaagag caaaaggttt tatagttgaa
     3121 ctttttccag gagatgcacg cctacacgaa gctttgctca aattttctcc agacaaagtg
     3181 attaatttag ttgactcagt gtatggtaag gaatatttgg ctgggaccat tccagcgacc
     3241 ttagaactgt tgcaaattcc ctacactggt agtggcatgt tggcaatttc tattaatgct
     3301 aataagtatt taactaaatc cttgttagag cagtatgggg tgaccactcc caagtatcag
     3361 ttaattaaac actctagcga tgaaattgat gaagagttag attttccttt atttattaaa
     3421 ttgaatgaaa atcatggttc tttggaggtg tcagatggtt cggttggttt tgatgaaaaa
     3481 atggctaaaa accaaattga ttatttgtat aaaacctatc agcaacccat tttacttgaa
     3541 gaattcattg ctggaagaga aattactgta gtgttagtcg acggtggcaa caccaaaatt
     3601 tatgcggctg aaaaaatctt tcatcgttct agcgacgatc cgttcaagaa aattgtcacc
     3661 tttgacgaca attgggtgtc caaagaggaa actatcactt acttaaaata tgaattaccc
     3721 gatccagtga aaagagtagc taagacggtt ttcaatattc tcaaaattga agactatgcc
     3781 aaattagatt ttagactaga tgaagctgga cgtcattact tgattgatgt taatgtcaat
     3841 ccagctcttg gtcctagcga gtcgtgtgca attggtagta ttttggaact atatggggtt
     3901 agtttttaca ctttattgga taggttgatt aacaactagc ttgtgtctaa gaataatttc
     3961 tatcatttaa aaaaagttga ggatttttgc ttaagagata atcgctgggt atctttagat
     4021 tggcgattaa agctaaataa tttggcccaa ttgaaaaaag aattatctgt agccaaacaa
     4081 tacgctaaaa aaagatttgt taataaaatt gaagtattat tgccgattac aattccccaa
     4141 gaattagccg aacttttata cagtgaaaat tttaaacctg ctttggttga attaaaattt
     4201 gatttaaaaa aatttactca agcttcatat aacattgcta atttgaactt tagtagtcaa
     4261 attaaaaata aatcggcgct caaagatttg ttgatcgaac aggctgattt tcaccaccaa
     4321 aatttgccag attactattt agctgccaga gaaattaatt ggttttttta tttgagacaa
     4381 tttttaaatg acgctacgaa agaaaatggt ttattgctgt taatcgaaga taaaggagat
     4441 ttgagcggat tagttttagg tgaatacttg ggcaaaacag cgtttatttg ggaattggtc
     4501 gtctgtcaaa aaaaacgtca ttttcatttg ggtagttatt tactcaatca atatttagct
     4561 aaattgaaag atttgggcat tgctagagtt tatttagaaa cgatgagcaa ttcctatgcc
     4621 cggagttggt atcaacatca gggttttacc gaagtcagcc aatcttggtt ttctcgtcta
     4681 aaataggaga atctgttgca ataattagat ttgccaataa aaattaaatc tggtattttt
     4741 cacgactttt ttcaattaaa gagtaaagac aaccacaata attttgttta taaaagccct
     4801 tattaactcg attacggtca agctttgtca gtttaggaaa ataaaaatca atttgatact
     4861 ccgcgcttaa tcgttcaaca atttttttaa tttgctctag atcttgataa ttgctcgaaa
     4921 gtaaagtgga gctgacgagt gagatatgtt cctgtttggc tttttcaaag agggctctca
     4981 atcttaattg ccaacaatta ggacagcgcc gtgattgagg aatgaccgtg aaaatttgct
     5041 gagattggtt tttttcacgc aactcattga ttgaccgatt tgtagccagg gcgtgaaaat
     5101 aatcttgtgg caggtaattt tccacaaaaa ttttaatgtc tggatctaaa cttttcacga
     5161 cttttttcac agcttgcaac cgggcattaa actcttctct tggatagaga ttgggattat
     5221 aaaaaaatat ttggctttgc ttttgttctt cactggacaa agcttgataa aattttaaga
     5281 agcaatcggc gcaacaggta tgaattaaca tgtttttgac tataattgac ttttctcaat
     5341 aaacagtata ttttacagct agagatgtcg caaattcaag aggttaaaga cgcgaataat
     5401 attgttgaga ttgtgggcga aaaaattgat ttacggcctt cgggcagtag tttcaaagca
     5461 cgttgtcctt ttcacagtga aaaaactcct tcattttttg ttcatcccga gatgcagcgt
     5521 tatcgctgtt ttggctgtgg ggctactggt gatgtgttag attttttgca gaactatgac
     5581 ggcatgacct tttatgaagc tctgaaatat ttggctgatc gtgcctcaat tactttaaaa
     5641 gattttaatc ggaccagtga agatgaagaa cgggagcagt tgttggggat tcttaattta
     5701 gctaaagaat attatcacta cctgttaact caacatgagg cgggtaagcc agcgcttgct
     5761 tacctaaaag aacgagggac caatagtcag tcaataaagt tatttaattt gggctattct
     5821 ttgtcaagtt gggatggctt aattaagtat ttacaccaaa aaaagaagta tccgctagaa
     5881 ttaattgcta aaactggttt gatcataaaa ggtaagggtg atcgttatta tgatcgcttc
     5941 cgcggacgag tgatgttccc tttaaaaaat catcggggac aagtggttgg tttttctggc
     6001 aggttactaa ctgctcaagc gaaagaagct aaatacatta atagtcctga aaccagtctt
     6061 tatcacaaat caaaattact ctatggttat agtgagctct ttagagaaat aagaaaagct
     6121 aataaagtgg tagtggttga gggtgagttt gatgttattt cctcagctca agctcatgta
     6181 aataacgtag tggcgatcaa ggggagtgcg ctgacggctg atcaggttga tttattggca
     6241 agagttagtg acagtgttat tttggctttt gatgctgatg aagcaggcat taacgcctgc
     6301 aaaagagcta tcactttgat gaaagataaa gacatagatt tgcgagtgat agattttcca
     6361 gctttacacc aattagcgac agcaaaagat ctcaaagatc ccgatgattt tgctcgacaa
     6421 gaaccaaaac tttggagaca agctgttgat caggcgattt cgatctacag ttttttactc
     6481 aataccaccc tagctcatta cgatgcagaa actccagacg gaaagcggaa aattattgat
     6541 gaattagctc cgatcattaa tggtattgat catttggttg agaaggaatt ctatttacaa
     6601 aaactttcag aaaaactcaa tgtcaaactt ggattaatta aagaagatat cgctcgtgtc
     6661 gcggaaaaga agaataaaaa aacaactgtg gtaaaagtca aaccaccaat tgagccaaac
     6721 aagcaaaaag ccaaaagcca tcaagaaatt atggaagagt atttattgtt tttacttttt
     6781 catttagaaa aggatgcatt tgaggaggga gttaacaaca ttattggttt aaaatggcag
     6841 actcctggtt taagcaatat tgtcgatatc ttaagtcggg aattaaaaaa ttttgattta
     6901 gaaaaattta gtaagcgttt agccgaggat ttgaaggcaa tcttaatgga aatcttactc
     6961 aacccagaat ttgaaaaaaa tataaaaaat actacgccaa aaagagaatg gcaaagagtt
     7021 ttagaacaag tgaaaaagaa tatagttcac agcgaaattg aagcaataaa tcaagaaatt
     7081 aatgaactag ataaaaaaaa tcaaagaacc aaagacgagg aacaacgttt agatcaattg
     7141 ttagagcaaa ttgtgcaaat gcaacgtcaa ttaaagagtt aaatttattg ataaactgac
     7201 tcttgtttag atacccccat agggtataat aaaactatgg cttttattag aaataagata
     7261 tctttagcaa aaaaagatca agttattact cagctgaaac ggatacgtgg gcaaattgat
     7321 ggtctaattc ggatgtatga agatgaacga ccgtgtattg aaattgctca acaaatttcc
     7381 gcagcacgta actctttgtc cagtgttgct agaaatgttt taaataatgc agctgataac
     7441 tgcatcaaaa ataaagacca aaaacaatta gatttaattg taaaagaatt attaaagtaa
     7501 caattattaa gaaaggtgct tatgtcagca caacatttag atagtagtaa ttttcagtcc
     7561 actttggata acgcgacaac accagtgatg gttgattttt atgcagactg gtgcggtcct
     7621 tgtcagatgg ctgcgcccat tattgataaa ttagctgatg attatcaaga caagatgatg
     7681 attgtcaaac tcgatgtcga tgaggccaga gatatcgcta gtgaattcaa cgtgatgaat
     7741 attccaacag tgatcatttt caaaaaaggt gatgacaata aaatggtgga gtttgatcgc
     7801 aaaatcggct ttcctggtga gactggttac caaaaaatga ttgaagaggc tctagcctaa
     7861 agcaaaggat ttaactagaa tcgaatttat taatttggat gtactataat ggttagtgga
     7921 tcgaaaaata tgaaaaattt gaaagtagcc gttattgggg ctggtcctgc aggtttcact
     7981 gctggaattt atttaggtcg tgctttatta aaacccgtcc tgtttagtgg gataaatctc
     8041 ggtgggcaat taatgtttac cagagatatt gaaaattttc cgggatttcc tgatgggatg
     8101 cgtggcccgc aatttatgtt gagtttgcaa aagcaggcag tgaagtttga tgcagaaatt
     8161 tgtcatgaac aaattactgc agttgatttt agtcaacgac cgtttcgttt gtggaactct
     8221 ctaccagcag atatggaatt tgctgattgg cagcatttag ccaaagcaga gttgctcaaa
     8281 atcactgaat caatcaaaaa agaaaatgag gctgcgtata gcgccgacag tgttttgatc
     8341 agcaccgggg ctgagcacat gaaactagga gttcctggag aagaagctta ttttggtaaa
     8401 ggtgtagggt tttgtgcagt gtgtgatggt gctttttacc gcgataaaaa agtttttgtt
     8461 gtaggtggag gggatacagc tttgagtgat gccttgactc tcagtaagtt tactgatcaa
     8521 gtattcttaa tccatcgccg ggagcaattc cgggcttgca aaattttgca acaacgagtt
     8581 gctgctgatg agcatattaa aattttctgg aacaccgaaa ttaaagaaat tgttggcgat
     8641 gaacagcgag tgacagccct aaaattgctt agagatggaa aagaagagca agtcgctgcc
     8701 gatggcatat ttatcgctat tggctatcag cccaatagtg ctctttttag taatcaggtc
     8761 aaattagatg atcattcata tattttgact cggcagtttg gtagtgatca agccttggca
     8821 gaattgattc aagaacctac gccaactact caagattatt ttccaaccat gacttcggtt
     8881 cctggagtgt ttgctgcggg tgatgtgact gatagtcatt atcgtcaagc agtggtggct
     8941 gcaggttcag gagcaatggc tgctctggat atcgaacgct ggttagagtt ggagtcaaat
     9001 aaataatttg aaaaaatttt attcttgatt aacaaatctt tttatttcag cccacagttc
     9061 gggatggtct ttagccacag tcaaattagc tattaaccag cctaatgggt aaccaccatc
     9121 aagatattct ccttgtggtt gatgaatcac tactttttca gtaagagcta gttggctaat
     9181 ggtgtcagta atatagagtt ctccagatgc aggattgggt tgttgaacgg ctattaaatc
     9241 aaaaactgat tggggaagaa tgtacttgct aatattggct aaattggacg gggcggttcc
     9301 tggtttgggt ttttcaacta aggcttgtaa gtagcggaag ttgttctctt cgctcagttt
     9361 ggccactcca taacgaggta aatcactttc tgctttttcg atacaagtaa ctaaagctgc
     9421 tgcaccagat tgctcataag tttgcatcat ggcagccact tcactagttc cattgtgtcg
     9481 ataaataaaa tcatcaccca ttaacaccaa aaaagcttct tcgtcctgca aatattcttt
     9541 ggctaattgt actggtacag cagtcccata atcatcagca tcactttgtt taataaagtg
     9601 aaaattagct tttgtatgta aatctttgat tagatcataa agttctaatt tgttattatc
     9661 ttttaaatat tggtgtaatc tttgattttc tcgataataa tgcaacactt gataattgtg
     9721 ttcgctgaca acaaaaacaa tgtcagtaat acctgcctta acgcaatctt caacgacata
     9781 atcgatcaag ggtcgattaa taattggcaa catttccttt tgaatagttt tactgatagg
     9841 taaaaatctt gtgccaaatc cagctgaggt gatgacggct ttacgaattt tcatatttta
     9901 tctttataac atacttttgt ataattacta agtttatgca gtttatttta gcatcacaat
     9961 cgccccaacg ggcaaggctt ttagccaccc taccttatcg atttaaaatt tatccagcaa
    10021 atattaacga atcggcgatc aaattcaaaa accccagaga taaagcgaaa aaaattgccc
    10081 tagccaaagc caacgccgtg gcaaaaaatg ttgctggtga ggcgattatc gtggcggctg
    10141 acaccttttg cttatgtcga ggaaaaattt tagaaaaacc caaaagcaag gcagaggcga
    10201 gaaaaatgct cgagttgcaa agtcaacagg agctaaaagc gattactggc tatgctttta
    10261 tttaccaaaa ctcctcaaaa aaaattattg aaaactccag tgtggtaacc caagttcgtt
    10321 tccgtcattt atcgtctcct gaaattgaaa actacattag tcatcaacca gtgttaactt
    10381 ggtcagcagc ttttagtccc gcttatgatg ctggaatggc tctactggca tggtcaaagg
    10441 gtaatttgac ggcttttact catggtttac caattgattt gttagtggaa tttattaatc
    10501 agcatttaaa cttaggcgta gaaaacaaac atcacactga taaaccataa aaagacggtc
    10561 actaataaag ttttatcctt caatagaaca tctgctggag cttcacctcg attttcttca
    10621 taaactaatt gtaagtagcg cataatgcca aaaatgacca aaggcagagt caacatcaac
    10681 aatttttgcg attgtagggt gcgcggtaaa ttaatgacta aattgggaaa gcgttcatat
    10741 agtaaattta aaccatcacc tggagaactt tgaaaagcaa aaagagcata agtgagccaa
    10801 gtagaggtgg caaaaatact ggtgtattga tctaaaagtc gagcactata agtattaaga
    10861 ctttttctaa cgaaagagag tttgtgctct gtcattaaag ttcgttcact ttgccgttta
    10921 ccaactgcca agaataggga catggaaatt acggtcaata aaaaccaaac actcatgtgt
    10981 aagttcacca ccactgctcc cgcataaact ctaattaaaa atccagcagc aatagagatg
    11041 atatctagaa tagcaatttg cttgaaatac aatgagtaaa ggatttgtaa aattaagtaa
    11101 gcaaaaatta aaagcttaaa cactaaagtt agaggtaagc ttagccaaaa cactagagct
    11161 aaacaaaacg ctatggcaaa aatcgctgtg ggaattgata gtttgccaga tgctaaaggt
    11221 cgattttttt tgaaggggtg tttacgatca gcttcaatat caaacaaatc attgagaata
    11281 taagtgctac tggtaagcaa acaaaaaatt ataaaggcgt aagaaactaa gtaaaaatat
    11341 gatggaccat cttctggctt ataaaagaaa aatccagaga aaactaaagc ggcatagaga
    11401 gcaaaatttt ttaaccactg tttgggccga gctgttttaa gcaaaaggaa taactgactg
    11461 ttaaaaaatg atttcatttt ctgagcttat tataaatcaa aaaagcaaaa acaaataaac
    11521 taacaagaac aatgctaata attttaccgg tagtgttaag aaacaaagat aaaaaaccca
    11581 taaaaaaact agtcaaaata taaaaaatgc taatgcgacg cggatcccag ccaaagttgt
    11641 ccattagttt gtggtgtaga tgacctctat caccccaaaa tggtgattta ccagctaaaa
    11701 ttcttctaac gattgtaaaa ataccatcgg cagtaggaat ggctaagacc atcaacgtag
    11761 tcgccacttt cgctccagaa agaatggcta aaacacttaa aaaataaccg gctagcgagc
    11821 ctgctccata gccaggcata attttttgcg gataaaaatt ccaatacaat aagccagcaa
    11881 aagcgcctga gacgattaag gccagttgag caacattaaa ttggctagga gcatcttgaa
    11941 aactattggc taaaactgca ataaaaagac aagccaccat gacaaagccc ggcaattgtc
    12001 catctactcc ctttgaccaa ttaataatat tcatattcca cataataaat aaaatggcaa
    12061 aagcactaga aaggagccaa atgtttcttt cttcaccaaa taagacaaag tgtagttgcg
    12121 gttgatcgag gtgaatgaca ccaggtccca aaggattact tacataggca atgccaatac
    12181 cggaaccaac cacgattaaa gtggctaaca gaccggttaa gaggcgccag tacgggctaa
    12241 tattaaaaat gtcatcaata atgccaacaa tagtcagaat gaaagcccct aaaaaaattg
    12301 ctattaaata tttatcaaaa tgtaaaaaaa taatagaact taaaaacact gcaataaaaa
    12361 ttaagatgcc accgcctcga ggcacaggtt tagtgtgagt aactttaaca tgatgatttt
    12421 ttttagggtc atcaacccag tgatgttttt ggtaaaaatt tatcaccaat ggtcccaagc
    12481 taaagctaat cagaaaacta ataattaatg gccacataac ttaattaaga ataataagta
    12541 atcgcaataa aattgctaga attaaaattt ggatggctac cgtgcttagt agaaaaactt
    12601 gcaaaatggt gggatgagca gttttaaatt ttttgataat gaagaagtgc aaggcattaa
    12661 tcagagtgga catccccggt aatataaaaa tcaattctcg attgaccaat tgttggtcgt
    12721 caggtaagga ataaaacagg gggatggttt tttgtaatga agaaaaaaag attagtgaaa
    12781 gagcaagagt actaatatta gcaataagcg cataaagagc taattgacgg aaattttttg
    12841 gtaaaaaagt taaatattta aactcttttt ttcgactagc agaatctctg aagctaacgt
    12901 ccataaccat attataaacc ttattttgaa agcttttatt tcttcagaaa taagttgaca
    12961 tgatcaaatt gtgaatttac taagtagttt tcttttagat aagtattcaa ctgaggaaaa
    13021 gtatgctgat cgtttttcat gacgacaatt aactttggtt cttcctcttt gatctgagcc
    13081 aaagtgccct cgtaatcggc aaaatcttta atgtgaaaag caacagtaaa gcgtgaagtt
    13141 ggcacggttt tggtttgagc ataaagcata gcattatctc cccaaataaa aatcttttct
    13201 aaagctaatt cgtcaattaa ggtggccaat ttttgattat ctctggttag attattaaaa
    13261 gagtagtcat actccgcttt tgcaatttgc ccagtggcca ttcgccaaaa ctgactatag
    13321 tatttacttg ttgaataagg tttaaaacct aaagtgagca tcacaaaaat agtcaaagag
    13381 attagagcta aaccagtgac aagactcttg tagtgctttt ttacacctac aagcttcttc
    13441 tgaaagtgac tacccatttc tactaagagt aaggcaaatg ggggaaccat ttggataaaa
    13501 taatgcgggt atggacgatt cgataacaaa gtggcataga gggttgcggc gaaccatatg
    13561 cttaaaaatt gaaaacgttt atttaattct gtactgttta gagcaattaa aatcaaaaat
    13621 gagaaaaaat aaagggtttt accgagcaaa gaaaatgaaa aatccataaa ggcagagtta
    13681 aatgatgggt tccaagattg gctataccgc aaattatata aaagaccata ctccaaataa
    13741 tctcgtcccg atccaattgc ttgataataa atgatagaaa ttaaaatagg tactacaatg
    13801 ccagcagaga aatataataa tctccatagc aattgaccgc aagtcttttg ccaatctttg
    13861 aatgttttat tgactttgag agcatctgct aaaagcaata aggcaaaaat cgacaagaat
    13921 ggcactagat ccaataacgc tggaactttg gttaaaattc cgagagagaa aagcacgcca
    13981 gaagcaaata ataataaaga ttcttttttc ttttctttgg gccagctgat ttttttgcta
    14041 aaaaagttct gccagatttg agtttgcgaa aataaaaaag ctccaactat gacaaagccc
    14101 aaaacaaaca attctccatt gggaatattt ccttcgagcc agggaaggct ggttaacagc
    14161 accatgatta gtgtagcgat aaaactgggt agttcttttt taaataattt tttagcaaat
    14221 agccagaaaa aagtagtggc tacgaccatc caaaccaaat tgagtaagcg gaagtaaaat
    14281 tggttgggta ctctggcaaa ttgataaatc aacggggttt tatgatcaat gatggttgtg
    14341 taaagttttc caccatgatt gagtgcattg ccaacggtca agtaaatggc ttcatctccg
    14401 taccaatatg gttcaaaaaa gttaggaatt ctgagtaaga ctaacaaaaa caagttaata
    14461 aacaaagcgg tttgctgatc aagaaaaaga tcaatttttt tgatggtttt tttaaatgac
    14521 atactttcca ttttatctgg aaaaaagcta atagtgcaag tgctactttg ttttacttgt
    14581 ttgtttgggg attggccact ttttcagtca ctcgatattc gagatattta ccaaacatca
    14641 acctagtgtg agcatccatg cccggtaagg cactaaaaaa gaagccgaca actggaatca
    14701 ataaaaattc taaaggctga ctgatatatg accacaaatt ttttttaggt ctcggtggtc
    14761 gatttttagc atcgatgatg aaaatcgaaa gcatcgaaat cattgacatg gttaatagag
    14821 tggaagttac ttggggcaaa gttttaccaa tgatggtgcg attaaattgt tgattaagta
    14881 atggaggtaa catcgctgag acagtgacgg caaaccagtt aactggccat aaaaaatgat
    14941 cttcaatgac gtgtaaaacc cttagggttt tgtcaatgaa gggg
//
LOCUS       DAOSEW010000004        14510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_3427_length_14510_cov_4.304670, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000004 DAOSEW010000000
VERSION     DAOSEW010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 14510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14510
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3427_length_14510_cov_4.304670"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="PLJ02_00330"
     CDS             <1..1026
                     /locus_tag="PLJ02_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04908.1"
                     /translation="FFFLSMAMVVAGAVSAQSLDALSINVSPPVSYLYVKPGAGISAP
                     IHLENQGRYTVNVTPQLVDFRPNHETGQVVLQQTTDFKYLSIAGDQDQWGKSFIIRPG
                     ENYTLPLTIAIPADFPQGEYYLSVLFNVEQMLIPDLENSANTILSGVVASHLVLAVNL
                     DETDRSQIVIKDFKLPKVVDSLMGINFQIWAKNVGLNAGPVKGQLTISHWPSLSEQVY
                     EFFPDMVLAGSQRQVRGMSKEDLAQLEKLAKQEELIVANGDDFLTMQSQFLEENLISD
                     FSYKRPFLLGTYDFRLELGEEEQLERVIALPFSIVIVIISLPLFYRFFVFLIRSMTTV
                     KRTKRQE"
     gene            1106..2557
                     /locus_tag="PLJ02_00335"
     CDS             1106..2557
                     /locus_tag="PLJ02_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04909.1"
                     /translation="MKLKKQLFIFLPVILLIIFFARAANLAPVQSASLGNVTVLLNNS
                     RFSFVTALDGTQTIGSSLITIDTANYPSTSVLQAQSGDVLRIGTAGHDYNVATTIDDA
                     TDNKLSLTSGLLTGDVADDLPVYATQSSTMTVKLTTVSALPNGKIRILVPARDATAAN
                     ADGVPDDNGFDFGAVTPASITCPVVYPTGYGSWTATSARADGTIQMGGVDYHVFTCQY
                     VGTGAVGTIYDGTTYNAFVINNLINPSPKTANLGVADTYSVIVQHLTSDDVVVDQTVT
                     KIAVIDAVRVSATILPQLTFEIIGVTAPATKCGNSITKSTTPLAVPFGDLTVGQFSDL
                     AQVLRVTTNAENGYQVTAIQNDQLGRDGRACPNPVADPACIVDANVSGMSYSAAANWS
                     TISGNQYGFGFTLGSYTVGVTPAFYYNQGGLAYNARHFADLSAGNSPQLIFSRGSATL
                     EDLVDVCYRLTPAATNVAGDYENYIVYTATATF"
     gene            2580..3605
                     /locus_tag="PLJ02_00340"
     CDS             2580..3605
                     /locus_tag="PLJ02_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04910.1"
                     /translation="MKPSCPPKKSKLLIFLISFLLFSFLLRLPVFAQESVILTVKPVR
                     IGDDNSLLLAPGEKKQIQVKVENNSDRDLEIVSQVFDFIVADDGTTPIPVIDQQDNRW
                     SLASWITLAPAYNYLRAGGSATVNVLIEVPEDATPGGHYAMVLHSPNFTGVEGQSGAG
                     ISQRAGTLIYVLVDGAITETAYVSRFSVPSFLEKGPVPMEIKIRNESDIHITPSPKIT
                     VKNIFGKEVATMVLDAKNVFPFTERDFSDVWDKKWGFGPYTLSLEAAYGIQGQTMTAQ
                     AVIWLIPVTIIIIILLSILLIMAVVTMLKKKKEQQRRALLTKAEDATKAENAKRETQP
                     VDDQKQQ"
     gene            3586..5784
                     /locus_tag="PLJ02_00345"
     CDS             3586..5784
                     /locus_tag="PLJ02_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04911.1"
                     /translation="MIKSSNKQLLADFKKNKNLQQINDLVKSFLLVLWCLVLSLGAVF
                     AQSVPAVFAQSTPAIPDVNGEIKVTARMPDIVAPSTPILINPEDGANLDNAHPVFQWY
                     ESTDNQAMYYYQFILDGHVWFDNIPLTDFANDDYSLDYDAALGIYTLVAKDPLAHGSH
                     SWRIVAWDYGDNSASSTTWVFHIFTEDPNFTIKKIGDVNVNISAANPDSVPVTAISLF
                     ALDPLANEPWIIALGDANLKVDLVVTIPNRASQFYSQYTDGDGNWALQLSILPRNQII
                     RLDFNISDVVGHVSYIRNLFIIIQQHYWPPTPIPTMTKPPLTGFPSTILPSGTIYPSG
                     TVQPSGIQPSQVPVTPLPSPGIKIPIIPPKEIVHEVVEEVKASLPTRIATLLTELSRS
                     PIWNFLAQFFALFLALLVPLVTYILVLLKFYQDLSLTTLKKVFIALWPWSQRRQHLVF
                     EYHDSQAAPLVRIELLDAANKEVLDWQISNYLGQFSSFAWPSQPVVLKVKDNNFYYPI
                     GIDKPSYLRWENFYQSEAFVVGKNDHQPTAASFDPKRSLAIPTLMAQGKNNLPFIERI
                     RIILAYLLTYPWWFWALSLVVVLPFALRYPSIWNYLALLYYLAIALGKYLYRRGSTIW
                     QFRVVSSNGYQLNQNVILIINDLTQGFSQALVVAVQESASPKLHLLKSDFVFSLQARD
                     YAFWDGQRVISETEYKVNSEVIDEFKLHRINDANRSLKLLQPHCQLMPRQ"
     gene            complement(5729..6871)
                     /gene="mnmA"
                     /locus_tag="PLJ02_00350"
     CDS             complement(5729..6871)
                     /gene="mnmA"
                     /locus_tag="PLJ02_00350"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOI04912.1"
                     /translation="MAKKVLVALSGGVDSSTAAYLLKQQKLDVSAVYIKFYASNKKQA
                     QIIDQESQVAKKIAEALKIPFVMLDFAQEQKEWVIDDLLNSYQHGLTPNPCIVCNKLL
                     KFGRLLDWAKEQGFSHLATGHYAQIIQKNHELYLAAAKDKSKDQSYFLYQLNEAQLKQ
                     LIFPLGLLTKTAVREIAQKAKLNHLKRESFDICFLRDSNLRQFLANNIQENPGEIVDQ
                     NGLVIGQHRGLAFYTIGQRHGFVIDHQALKKSQTINYDKNRAPALLVVNKIPEKNRLV
                     VAEKAASLVNKFTIKELHFINNSQQQLWQEKASFYALVKIRNQGKLIPCQVKTNGNQL
                     LVKTQDKIFAPASGQSAVFYRDTRTNLLVIAGALIDNGVGEASNSD"
     gene            complement(6873..7838)
                     /locus_tag="PLJ02_00355"
     CDS             complement(6873..7838)
                     /locus_tag="PLJ02_00355"
                     /inference="COORDINATES: protein motif:HMM:NF019626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1704 domain-containing protein"
                     /protein_id="HOI04913.1"
                     /translation="MIKLKDLLPTNLSTEKEKFFAQGGNYNPQFQYQNSITKSGLDYY
                     GRARWKYLHLAKKIVNQAQKEQLFLKDQSLNSNLDQENTQKLITNYLNDYALANKYQV
                     VFNEHLTSRFAVNFKENFIKIRLPINFSKKSLDNILNHELGTHVLRQENYLQQPWYHR
                     KKEFGFANHLRTEEGLAVIHQHLNSDQQLLYQTALSYLGCHLASRADFKTVYQFFYHY
                     FKNPQKAWSQALKSKRGLKDTSLAGGFTKSLVYLEGVVQVSQYLHRHHYDPSPLYYGK
                     LAIQDLQKALKMNPHYQLILPKFYTDNPSAYQEKVATIAKRNFIF"
     gene            complement(7831..9126)
                     /locus_tag="PLJ02_00360"
     CDS             complement(7831..9126)
                     /locus_tag="PLJ02_00360"
                     /inference="COORDINATES: protein motif:HMM:NF025755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-transfer associated ATP-grasp
                     domain-containing protein"
                     /protein_id="HOI04914.1"
                     /translation="MPNKANPRDLLGINARNSLYVSRNSRKAKAIAHSKYATKILLNE
                     NNIPCAEIYGILGTDEDINDFAWQKLEGDFVIKPTNGHAGTGVIAFRNKINDEHWLDV
                     LGEKWSVDDIKLHCRDILAGQYSIHGSNHNVIIEERIPIHPKLLKYSYKGTPDVRVIV
                     FNRVPVMAELRLPTEESGGRANVSQGAIAVGIDIATGITTHAIAHKKQKIHYLPGTKK
                     KLNGIVIPNWQKVLLTAVRASEVADLTYSGIDIFLHKIKGPMVVELNAAPGLSIQAAN
                     QAGLRRRLERVEGLNVVNPEHGVKIAQALFASDFSGRIKVKSEISNISLYEEILLFGD
                     EKQKISTTAFINTGRLHSAIARSLAGELGLLDIEDLLWFQKEKEEGNLPVVEVKFKLK
                     GQIIKTTMLVSNKLNHRQHKIEIGRKDLDGFIISPKKDD"
     gene            complement(9136..10191)
                     /locus_tag="PLJ02_00365"
     CDS             complement(9136..10191)
                     /locus_tag="PLJ02_00365"
                     /inference="COORDINATES: protein motif:HMM:NF025760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM domain-containing protein"
                     /protein_id="HOI04915.1"
                     /translation="MYFKKILVSFLLLFSLYLIKNQFLPNGNPIGLTNVRAQTANPVR
                     FEGTDLESLEAIEESTPAAAATDSTSLASPSAEIVEKIQQKADEDITETTGRQKSVLA
                     AYLDEHPIGPLNPTNFLQKAIRQAIAQGLPASLVVIILIFPVIASLVAFARHVVGFKG
                     FGIYIPAVLSVAFVSTGLVTGIILFIAVLLSAMLARYLVQFLKMPLLPKTAMLLLIVS
                     IAMLLLLTVGSFFKIDLVLNVSIFPLLIIILLTENFMTSQMFASRGEALNVTFETLIL
                     AVVGTLLIDNEAIQRFVILKPELTILLVVVINFAIAHYTGLRILEYFRFKDLLRNKSK
                     SAKYKKIINDDSPGLEE"
     gene            complement(10211..10573)
                     /locus_tag="PLJ02_00370"
     CDS             complement(10211..10573)
                     /locus_tag="PLJ02_00370"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOI04916.1"
                     /translation="MKLLKPIIVLIVTIAILAWALPNVGYSDYLTLILAGVVMTLLEK
                     TIKPILKFLFLPINIVTLGFFSIIINVFILWLVTYFVPGFNIEPMIIFGLPLNQFFTL
                     LFVSALIGFCQRIINFIL"
     gene            complement(10604..11860)
                     /locus_tag="PLJ02_00375"
     CDS             complement(10604..11860)
                     /locus_tag="PLJ02_00375"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOI04917.1"
                     /translation="MLYVGKAKNLKKRIKSYTHYQQLSAKIKQLVKRAKIVRVKVLKS
                     ELQALLIEAELIRAHQPFYNSLLKDDKSKLYLLFTKEDYPRVLRLRKTDLKKQRYQQA
                     LAVLGPFASSYKVDELLKIVRPIFPWCNKQNKSNHQACLYYHLELCPGACCNKISPQA
                     YQENIKQLIKLLRGKNQALVKELTHKMQELAQTEQFEEAQKIKNKIELIKEVTEEQYQ
                     LQPELILPDLTRKKQEEALINLRHIMHERGLVDLKYRFQRIEAYDVSNTQGNQATVSM
                     VCFIDGYPEPSEYKFFKIRQLNQPNDYAMLQEAIARRQRHPEWGRPDLIVIDGGKGQL
                     RSVNRVWRQSQFFNCPVISIVKNPDRLVLITPKLDSTNQESWQTHIIHLAKNHPTLQL
                     ISQLRDEAHRFAKKQHTRLREQNLLQ"
     gene            complement(12477..13760)
                     /locus_tag="PLJ02_00380"
                     /pseudo
     CDS             complement(12477..13760)
                     /locus_tag="PLJ02_00380"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
     gene            complement(13789..>14510)
                     /locus_tag="PLJ02_00385"
     CDS             complement(13789..>14510)
                     /locus_tag="PLJ02_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014462634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOI04918.1"
                     /translation="TKTFDLIRQVFANTRDAQIRAYKTGRFSFNVKGGRCETCQGEGQ
                     IKIEMQFLPDVYVECDVCHGKRYNKETLEVYYQEKNIAEVLDLTIDEAYDFFKHQSLI
                     RHKLATLQAVGLGYLKLGQSAPTLSGGEAQRVKLAKELSIKSHDHTVYLLDEPTTGLH
                     FADIQKLLNVLHKLVKQDNTVIVIEHNLDVIKNADWIIDLGPGGGEAGGQVVATGTPE
                     SLSQNPNSLTGKYLKAELAES"
BASE COUNT         4208 a         2693 c         2902 g         4707 t
ORIGIN      
        1 ttcttttttt tgagcatggc tatggttgtt gcgggagcag tttctgctca aagccttgac
       61 gctttatcaa ttaatgtcag tcctccggtt tcttatttat atgttaagcc gggagcgggc
      121 attagtgccc ccattcatct ggagaatcaa ggtcgctaca cggtcaacgt aactcctcaa
      181 ttagtagatt ttcgacccaa tcatgaaact gggcaagttg ttttacaaca aacaactgat
      241 tttaaatatc tgtccattgc tggtgatcag gatcaatggg gtaagagttt tattattcgg
      301 cctggtgaaa attatacttt accactaaca atagcgattc ccgctgattt tccacaagga
      361 gaatattatt tatctgttct atttaacgtt gaacagatgt taattcctga tctagaaaat
      421 agtgccaata ccattttgtc aggtgtagtc gccagtcatt tagtgttagc agttaatttg
      481 gatgaaaccg atcggagtca aattgtaatt aaggatttta agttgcccaa agtagttgat
      541 agtttgatgg gaattaattt tcaaatttgg gccaaaaatg ttggtttaaa tgctggccca
      601 gttaaaggtc aactaactat tagtcattgg ccatctctca gcgagcaggt ttatgaattt
      661 tttccagata tggtattggc tggtagtcaa agacaagttc gagggatgag taaagaagat
      721 ttagctcagt tggaaaagtt ggccaaacaa gaagagttaa ttgtggctaa tggtgatgat
      781 tttttgacga tgcaaagcca gtttctcgaa gagaatttga ttagcgattt ttcttataag
      841 agaccattct tattgggtac ttatgatttt cgtttagagc tgggagaaga agaacaacta
      901 gaaagagtga tcgctcttcc cttttctatt gttattgtta tcatttcttt gccacttttt
      961 tatcgttttt ttgttttttt aatcagatca atgacgacgg tcaaaagaac aaaaagacaa
     1021 gaatgatttc tctatcaatt tatagagaaa ttgctcttga cttgcagcca aaaaacagct
     1081 taaacttaat aacaggtacc aaaatatgaa attaaagaaa cagttattca tctttttgcc
     1141 agttattctc ttaattatat tttttgccag ggcagccaat ttggctccag tgcaatctgc
     1201 gagtttagga aacgtgacgg tattgcttaa taattcccgt ttttcctttg tcaccgcttt
     1261 ggatgggacg caaacgattg gttcatcatt aattactatt gatactgcca attatccatc
     1321 aacttcagtt ctccaagctc aaagtggtga tgttctacgg attggtactg ctggtcatga
     1381 ttataatgtc gccacgacca ttgatgatgc cactgataat aaattaagtt taacttctgg
     1441 attattaact ggtgatgtgg ctgatgattt accagtctat gcgactcaat catcaaccat
     1501 gacggttaaa ttgaccacag tcagtgctct gcccaatggc aaaattcgta ttttagtgcc
     1561 agctagagat gcgacggcgg ctaacgctga tggcgtgcct gatgacaatg gttttgactt
     1621 tggcgctgtt actcctgcta gtattacttg tcctgtcgtt tatcctaccg gttatggtag
     1681 ctggactgct acaagtgctc gtgccgacgg gactatccaa atgggtggtg tcgattatca
     1741 tgtttttacc tgtcaatatg tgggcaccgg agcggtgggc acaatttatg atggtaccac
     1801 ttacaatgct tttgttatca ataatctcat taatccttcg ccaaaaacgg ctaatctagg
     1861 tgtagctgat acctattctg taattgtcca gcatttaacg agtgatgatg tagtggttga
     1921 ccaaacagtt accaaaattg cggtgattga tgcggtgaga gtctcagcca ctattttgcc
     1981 tcaactaact tttgaaatta tcggcgtaac ggcgccggct actaagtgtg gtaatagtat
     2041 tacgaaatca accaccccac tagccgtgcc atttggagat ttaacggttg gccaattttc
     2101 tgacctcgcc caggtgttga gagtgacgac taatgctgaa aatgggtatc aagtaacagc
     2161 catccagaat gatcagcttg gcagagatgg aagagcttgc ccgaacccag ttgctgatcc
     2221 cgcttgtatt gttgatgcta acgtgtctgg gatgagttat agtgctgcgg caaattggag
     2281 tactattagt ggaaatcaat acggatttgg cttcactctg ggatcataca cagttggtgt
     2341 gacgccagcc ttttattata atcaaggtgg attagcctat aatgcccgtc actttgctga
     2401 tttgtcggct ggaaacagtc ctcagttaat tttttccaga ggtagtgcca ctttagaaga
     2461 tctagttgat gtttgttatc gtttaacccc ggccgcgacc aatgtggctg gtgattatga
     2521 aaactacatc gtatatacag ctacggctac attttaaaaa taattaaaaa gaaaaaaata
     2581 tgaaaccatc ctgccctccc aaaaaatcaa aattgctgat tttcttaatt tcttttcttt
     2641 tgtttagttt tctactgcgc ttgccggttt ttgctcagga atccgtgatt ttaacggtta
     2701 aaccagtcag aattggtgac gacaattctt tgctcttagc tcctggcgaa aagaaacaga
     2761 ttcaggtaaa agttgaaaac aattcggata gagatttaga aattgtcagt caggtttttg
     2821 attttattgt agctgacgat gggaccaccc caattccagt gattgatcaa caagataatc
     2881 ggtggagttt ggctagctgg attactttag ccccggcata taattatttg cgggcgggcg
     2941 gtagtgcgac ggttaatgtg ttaattgagg tgccagagga tgccacaccc ggtggtcatt
     3001 acgcgatggt gttgcatagc ccgaacttta ctggtgttga gggtcaaagt ggagccggaa
     3061 tttcccaaag agctggaacc ttaatttatg ttctagtaga cggagcaatc accgaaactg
     3121 cttatgttag ccgatttagt gtgcccagtt ttttagaaaa aggtccagtg cccatggaaa
     3181 ttaaaattag aaatgagtca gatatccaca ttactccgag tccaaaaatc acagtcaaga
     3241 atatttttgg taaagaggta gctactatgg ttttagatgc aaaaaatgtt tttcctttta
     3301 ccgaacgtga ttttagtgat gtctgggata aaaagtgggg ttttggacca tacaccctca
     3361 gcttagaagc agcttacggt attcaaggtc aaacgatgac tgctcaagcg gtgatttggt
     3421 taattccagt gacaattatt atcatcattt tgctcagtat tttgctaatt atggctgtag
     3481 tgacaatgct taagaaaaag aaagagcagc aaagacgagc tcttttaacc aaagcagaag
     3541 atgcgacgaa agcagaaaat gcgaaaagag aaactcagcc agtagatgat caaaagcagc
     3601 aataagcaat tattagcaga ttttaaaaaa aacaaaaatc ttcagcaaat taatgatcta
     3661 gtaaaaagtt ttttgttggt gctttggtgt ttagttttat ctttaggtgc cgtttttgcc
     3721 caatctgtac ctgccgtttt tgcccaatct acacctgcta tacctgatgt caacggcgaa
     3781 atcaaagtga cggctagaat gccagatatt gttgcccctt cgacgccaat tctgattaat
     3841 ccagaggatg gagctaattt agataatgcc cacccagtct ttcaatggta tgaaagtaca
     3901 gacaaccaag ccatgtacta ttaccaattt atccttgatg ggcacgtttg gtttgataat
     3961 attccgctca ctgattttgc taatgatgat tatagtttgg attatgatgc agcattaggg
     4021 atttatactt tagtggctaa agatccttta gctcatggtt ctcatagttg gcgtattgtt
     4081 gcttgggatt atggtgataa ttcagccagt tctaccacct gggtgtttca tatttttact
     4141 gaggatccca attttacaat caagaaaatt ggtgatgtga atgttaatat cagtgctgcc
     4201 aaccctgata gcgttcccgt gacagcgatt agtctatttg ccctggatcc actagccaat
     4261 gaaccctgga ttatcgcttt gggtgatgct aatttaaaag ttgatttagt ggtaactatt
     4321 cctaaccgtg ctagccaatt ttatagtcaa tataccgatg gggatggcaa ctgggctttg
     4381 caattgagta ttttaccccg taatcaaatt attcgtttag attttaatat tagtgatgtg
     4441 gtaggtcatg tgagttatat taggaattta tttatcatta ttcagcaaca ttattggcca
     4501 cccaccccca tccccaccat gactaagcca ccgttaactg gttttccctc aactattttg
     4561 ccctcaggaa caatttaccc atcaggcaca gttcagccct ctggtattca accaagtcag
     4621 gttccggtta cgccactacc ttctcctggg ataaaaattc caattattcc acccaaagag
     4681 attgttcatg aagtagttga ggaggttaaa gccagtttac cgacgaggat tgccactttg
     4741 ctcaccgagc tgagccgatc cccaatttgg aatttcttgg cccagttttt tgccttgttc
     4801 ttggctttat tggtgccact tgtgacttat atactggttc ttctaaagtt ttatcaagac
     4861 ttatctttga ctactctcaa aaaggttttt atcgctttat ggccttggtc tcagcgaaga
     4921 caacatttag tttttgagta ccatgatagt caagcagctc ctctagtgcg aatagagcta
     4981 ctggatgcgg caaataaaga ggtgcttgat tggcaaatta gtaattattt aggtcaattt
     5041 tctagtttcg cttggccaag ccaacccgta gtactcaaag tcaaagataa taatttttac
     5101 tatccgattg ggattgataa gccaagttat ttacgctggg aaaattttta tcaaagtgaa
     5161 gcatttgtag ttggtaaaaa cgatcatcag ccaacagcag cctcttttga tcctaaaaga
     5221 tcgctcgcaa ttccgacttt aatggctcaa ggtaaaaata atctaccctt catcgagcgt
     5281 attagaatta ttctagccta tttattgacc tatccttggt ggttttgggc tttaagttta
     5341 gtggttgtat tgccttttgc cctgaggtat ccaagcattt ggaattattt ggctttgctt
     5401 tattacttag caattgcgct agggaaatat ctttatcgac gtggatcaac catttggcaa
     5461 ttcagggttg tttctagcaa cggctatcaa ttaaatcaaa acgtgatctt aattattaat
     5521 gatctaaccc aaggtttttc ccaggctttg gtggtggcag tgcaagaaag tgctagtccc
     5581 aagttacact tactgaaaag tgattttgta tttagtttgc aagccagaga ttatgctttt
     5641 tgggacggtc agcgagtaat tagtgaaact gaatataagg ttaatagtga agtcattgat
     5701 gagtttaaat tgcatcggat aaacgacgct aatcggagtt tgaagcttct ccaaccccat
     5761 tgtcaattaa tgccccggca atgactaata aattggttct agtgtcacga taaaatactg
     5821 ccgattgacc actagcagga gcgaaaattt tatcctgagt tttcactaac agctggttgc
     5881 cgtttgtttt tacctgacaa gggattaatt tgccttgatt gcgaatctta actaaagcat
     5941 aaaagctcgc tttttcttgc catagttgtt gctgactgtt attaatgaag tgcaattctt
     6001 taatagtaaa tttgttgacc aaactagctg ctttttccgc gactaccaga cggttttttt
     6061 caggaatttt atttaccacc aataaggctg gagcccgatt tttgtcatag ttaattgttt
     6121 ggcttttttt aagagcttga tgatcaatga caaagccatg acgttgacca atcgtgtaaa
     6181 aagccaaccc ccgatgctgg ccaatgacca aaccgttctg atcaacaatt tcgccaggat
     6241 tttcttggat attgtttgct aaaaattgtc taaggttact atccctaagg aaacaaatat
     6301 caaaagattc tcgctttaag tgattaagct tagccttttg agcaatctct ctgacagcgg
     6361 ttttagttag caaacccagg ggaaaaataa gttgcttgag ttgtgcttcg ttaagttggt
     6421 ataaaaaata gctttgatct ttgcttttat ctttagcagc ggccagatat aactcgtgat
     6481 ttttttgaat aatttgggcg taatgaccag tggctaagtg agaaaagcct tgttcttttg
     6541 cccaatctag taagcgacca aactttaata atttattaca gacaatgcaa gggttaggag
     6601 tgagaccgtg ttgataagag tttaataaat catcaatcac ccactctttt tgttcttgag
     6661 caaaatccag catgacaaaa ggaatcttga gtgcttcggc aatttttttt gcgacttgac
     6721 tctcttgatc aataatttgc gcttgttttt tgttgctagc ataaaattta atgtaaacag
     6781 cgctgacatc cagcttttgc tgtttaagca aataggcagc ggtactagaa tctactcctc
     6841 cagaaagagc gactaaaact ttttttgcca tattaaaaaa taaaattcct tttagcgatg
     6901 gttgctactt tttcttgata agcagatgga ttgtctgtat aaaattttgg caaaattaat
     6961 tggtagtggg gattcatttt tagggctttt tgaagatctt gaatagccaa tttgccgtag
     7021 tacaaggggc tgggatcata atgatggcgg tggagatatt gtgaaacttg tacaacgcct
     7081 tctaaataga ctagactttt agtaaaacca ccagccaaac tggtatcctt taaacctctc
     7141 ttacttttca aggcttgtga ccaggctttt tgggggtttt taaaatagtg atagaaaaat
     7201 tgatagacgg ttttaaagtc cgcccgacta gctaagtggc aacccaggta tgataaagca
     7261 gtttgataaa gtaactgttg gtcgctgttt agatgttgat gaataacggc caaaccctct
     7321 tcggtgcgta aatgattggc aaagccaaac tcttttttac gatgatacca gggttgttgt
     7381 aagtagtttt cttgacgaag gacatgggtg cccaactcgt gattgaggat attgtctaaa
     7441 ctttttttgc taaaattaat tggtaagcga attttgataa aattttcttt aaagttaact
     7501 gcaaagcgac tggtcaagtg ttcattaaac accacttgat acttattcgc tagagcataa
     7561 tcatttaaat agttagtaat taatttttgc gtattttctt gatctaaatt actgtttaag
     7621 ctttgatcct tgagaaataa ttgctctttt tgggcttggt tgacaatttt tttggctaag
     7681 tgtaggtatt tccaccttgc cctaccataa taatcaagtc cagatttggt gatgctattt
     7741 tgatactgaa attgaggatt atagtttccg ccttgagcaa aaaacttttc tttttcagtt
     7801 gacagattag tgggcagtaa atccttaagt ttaatcatct tttttagggc taatgataaa
     7861 accatcaaga tctttacgac caatctcaat cttgtgttga cgatgattca atttattact
     7921 gactaacata gtggtcttga tgatttgtcc cttgagttta aatttaactt ctactacagg
     7981 tagattacct tcctcttttt ctttttgaaa ccacaataaa tcttcaatat cgagtaaacc
     8041 tagttcacca gccagactgc gagcaattgc actatgtaag cgaccagtgt tgataaaggc
     8101 cgtggttgag attttttgtt tttcatcccc aaaaagtaaa atttcttcat ataaactaat
     8161 attactaatt tcacttttta ctttgattcg tccagaaaaa tcactagcga atagcgcttg
     8221 ggcaattttt actccatgtt caggattaac cacatttaaa ccctccactc tttctaatcg
     8281 gcgccgcaac ccagcctgat tggcagcctg aatagacaaa ccaggtgccg cgttcaactc
     8341 gaccaccatg gggcctttga ttttatgtaa gaaaatgtcg attccagagt aagttaaatc
     8401 ggctacctca ctagctcgaa ccgctgtcaa aagcactttt tgccagttag gaatgacaat
     8461 gccatttaat tttttcttag ttcctggtaa ataatgaatt ttctgctttt tatgagcaat
     8521 agcatgagta gtgatgcccg tagcaatatc gatcccgaca gcaattgctc cctgactgac
     8581 attcgctcta ccgccagatt cttcggtggg gagacgtaac tcggccatga ctggtacccg
     8641 attaaaaaca atgactctga catctggcgt ccctttgtaa gaatatttaa gtagtttggg
     8701 gtgaatagga atgcgttctt caataatgac attatggttc gagccatgaa tactatattg
     8761 acctgccaga atgtctcggc aatgcagttt aatatcgtca acactccatt tttctcccag
     8821 tacatcaagc caatgttcat cattaatttt gtttctaaag gcaatgacac cagtgcccgc
     8881 gtgtccgttg gttggtttga taacaaaatc gccctcaagt ttttgccaag caaaatcatt
     8941 gatatcttca tcggtgccta aaataccgta gatttcggca cagggaatat tattttcatt
     9001 taataaaatt tttgtagcat acttggaatg ggcaatcgcc ttggctttgc gcgaattccg
     9061 actaacatag agactattac gtgcattaat gccaagaaga tctctggggt tggctttgtt
     9121 aggcataaac ggcccttact cttctagtcc tggagaatca tcattgataa tttttttata
     9181 tttcgctgat ttacttttat ttcttagcaa atccttgaag cggaaatatt caagaatgcg
     9241 caaaccagtg tagtgagcaa tggcaaaatt aatcaccact accaaaagaa tggttaattc
     9301 aggctttaag atgacaaacc tttgaatcgc ttcattatca atcagcagtg ttcccaccac
     9361 tgccaaaatt aatgtctcaa aagtcacatt taaagcttca ccacgactag caaacatttg
     9421 gctagtcata aaattttccg tcaacaagat gataattaat agtgggaaaa tactaacatt
     9481 cagtaccagg tcaattttga agaacgaacc aacggttaat aacagcagca tagcaattga
     9541 cacaattaac aaaagcatgg cggtttttgg caaaagtggc attttcaaaa attgtacgag
     9601 atatcttgcc aacatggcag agagtaggac tgcaataaat aaaataatac cagtgactaa
     9661 gccggttgaa acaaaagcga ctgataaaac agctggaata taaataccaa aacctttaaa
     9721 accaaccaca tgtctagcaa aggcaaccaa tgaagcaatc actggaaaaa ttaagataat
     9781 cactactaaa ctggctggta aaccttgagc aatagcttga cgaatagcct tttgtaagaa
     9841 atttgtagga tttagtggtc caatgggatg ttcatcgaga taagctgcca aaacactttt
     9901 ttgacgacca gtagtctcag taatatcctc atcagctttt tgttggatct tttccacaat
     9961 ttcggcagat ggtgaggcca aactggtact gtcagttgct gcagccgcag gggtcgattc
    10021 ctcgatagcc tctaagcttt ctaaatcggt accttcaaat cgaacaggat tagcggtttg
    10081 cgccctgacg ttggttaaac caatggggtt tccattcggt aaaaactggt tttttattaa
    10141 ataaaggctg aataatagca aaaaactaac tagaattttt ttaaaataca taaggcctta
    10201 atatattatt ttataaaata aagttgatga ttctttgaca aaagccaatt aaggctgaaa
    10261 caaaaaggag agtaaaaaat tggtttaaag gaagaccaaa aataatcatt ggttcgatgt
    10321 tgaagcctgg aacaaaatag gtaactaacc acaagataaa gacgttaatg ataatagaaa
    10381 agaagcctag agtaacaata ttaattggta aaaataaaaa tttcaaaatc ggtttaatag
    10441 ttttttccaa taatgtcatc actactccag ccaaaattag ggttagataa tcgctgtagc
    10501 caacattggg taatgcccag gctaagatag caatagtgac aatcaagaca ataattggtt
    10561 taagtaattt cataaatggc cttatagttc aatccagtct ctgctattgt aaaagatttt
    10621 gttctcttag tctagtatgt tgttttttag caaagcgatg agcctcatcg cgcaattgac
    10681 taattagctg taaggttgga tggttttttg ctaagtgaat gatgtgggtt tgccatgatt
    10741 cttgattggt tgagtcaagt tttggggtaa ttaagaccaa acgatctgga ttttttacta
    10801 ttgagattac tggacaatta aaaaattgac tttgtcgcca gacccgatta acactccgca
    10861 attgtccttt gccgccatca atcacaatta agtctggtct accccactct ggatgtcgtt
    10921 gtctgcgcgc aatggcttct tgcaacatgg catagtcatt gggttgattt aattgacgaa
    10981 ttttaaagaa tttatattcg cttggctctg gataaccatc aataaagcag accattgaga
    11041 ctgttgcctg atttccttga gtattgctca catcgtaagc ttcaatacgt tgaaaacggt
    11101 attttaaatc gaccaagcct cgttcatgca tgatgtggcg taagttaatc agggcttcct
    11161 cttgtttttt tctggtcaaa tctggcaaaa ttaattctgg ttggagttga tattgttcct
    11221 cagtgacttc tttaatcagc tcaattttgt ttttgatttt ttgggcttct tcaaattgct
    11281 cggtctgggc aagttcttgc attttatgag ttagctcttt gactagtgcc tgatttttac
    11341 ctcgaagtaa tttaatcagt tgtttaatgt tttcttgata agcttgtggg ctaattttgt
    11401 tacagcaagc tcctgggcag agttccaaat ggtagtagag acatgcttgg tgattacttt
    11461 tattttgctt attacaccaa gggaagattg gtctaacaat ctttaataat tcatcgactt
    11521 tataactgct agcaaaagga ccaagaacag caagtgcttg ctgatagcgt tgttttttta
    11581 gatcagtttt tctgagtcgt aaaactctgg ggtaatcttc tttggtgaaa agaagataca
    11641 acttggattt atcatcttta agcaggctat tataaaaggg ttgatgggct ctaattaatt
    11701 cagcctcaat cagtagggct tgaagttcgc ttttgaggac tttgactcgt acgatcttgg
    11761 ctctcttgac taattgtttg atcttcgctg agagctgctg atagtgagtg taactcttga
    11821 ttcttttttt cagatttttg gccttgccga cgtaaagcac ctgtgagtgt tcgtccaaga
    11881 aggaataaac tcccggctgt gagggtaagg aggataaatc ctccgaccac aaaaaggatt
    11941 ctttgggggt tgctgatgcc gatttcgccc aggcctctga ctgtgaattc atagttaaaa
    12001 ttttctcctt cgcttaattg aatttcagca ttaatctgtg tttgctgacc aattaaacca
    12061 tccctattat aaacgctcaa gggaataatc aattcttgga agggagcaat gagtagcggc
    12121 cagtcagctt ctggaataag atcaactgtt tccccctcaa gactcagctt ggtaaaatac
    12181 caagtctgac cagttttatt gctgattatt aatgaatatt ttccggggat ggccaaattg
    12241 aacagttgct tttcttttaa ctgcaaatta atttctggtt caatgtcagg aaattcgttt
    12301 tggctgaatt ccacaaaaat ctttttttct tgggcttgga gatcggtttg ataagaaccg
    12361 gctggataag gcaacacact ggaaacacca ttaatggcga aagtgatgtg gttaagatca
    12421 aattttgaaa aataatcgat accaccagta gtttcttgcc aggtcggatc aatttcctgc
    12481 caactgttag ttacttgatt ataaaatgct ggccaagcat gcaacacatc gccaaccaca
    12541 ctaattggtc gtagttcctc gttattactg taagcatagc ccacaattct ttttgctgga
    12601 atttgtttga tacgggctaa agtaacgaat aaatcggtaa actcttgaca ggtggccaag
    12661 tttctatttt ctggcaaaaa actattggcc gctcccaaac gataaaattc cccatctaaa
    12721 cttttttcag tatagtctag cgtatcaacc acaaattgat taatgtcact aacactagtt
    12781 aatccactag cggcttcttg taattgtggg ttatcggttt cccaatattt ttgaggaagt
    12841 aagtgagctg gcagagcagg tggggtttga atatggggat cagcttgcag ggtaagcagt
    12901 acttgagcta aaagttcaac tttgactgaa ttattagccg gcacctcata tgtagcaata
    12961 aaattgccgt cagcatcaac tttaattgac tcgggcaatg gatcaagtag atgataatga
    13021 acttgttgat tgggggtttc tggcgggaga gtaatttgac ttaaggcatt ttgattatta
    13081 gggttgttta agtagtaact tagttttaaa tcaaaaattt gtctgtcgcc aaaaatggca
    13141 ctcactgcct gaccgtccag attgtcgtaa tgaaaattat caaaatcacc agcttttatg
    13201 accgtaaaat tactaggact aatgcgactt actggaccaa agatacttgg gattaataaa
    13261 tttaactgat actgttcgta ttgttcaatg tcggctaaag caggaatatt aacttccaaa
    13321 attttgccac taatattggc aatatcacca tctaaatagc taatgattaa ttctctagtt
    13381 ttaccttccc ctagaatttc ttcttcgaaa gtgaccccaa tgctagtttg accagtcagt
    13441 tttgtgactt ggggttctag agttgtgccg ttatctgttg ctttgatatt ttccaaattg
    13501 gtagaactaa caataatgcc ataacgatta ataaaatact ccggactaag attgcgaata
    13561 gtgaattttt gcgtaactaa agttttgcca ttggcattaa cttgataatt actttccaaa
    13621 ttgatgctaa atttttttgc ttcttcttca gcaatgacgg gtgttgttgc agaaaaaaga
    13681 ctgatcaaaa tgaagagcaa agaaaaaatt tttaataaaa aattcttttt cgcaaaaaat
    13741 ttgacttgaa atctcaccat taacatcact tcaattgtag cggaaaagct aactttcagc
    13801 caattcggct tttaagtatt ttccagttaa ggaattgggg ttttggctta agctttcggg
    13861 agtgccagtg gccactacct gcccacctgc ttctccaccc ccaggaccca agtcgataat
    13921 ccaatcggcg ttcttaatta cgtctaaatt gtgttcaatc acgatcactg tattatcttg
    13981 tttaactaat ttgtgcaaga cattaagtaa cttttgaatg tcggcaaaat gcaggcctgt
    14041 tgtcggttcg tctaacaaat aaaccgtatg atcatgactt ttgatactca attctttagc
    14101 gagtttaacc cgttgcgctt cgcccccact taaagtggga gcactttgcc caagttttaa
    14161 atagcccaaa ccgacagctt gtaaagttgc taatttatga cgaattaaag attgatgctt
    14221 aaagaaatca taagcctcat caattgttag atctaagacc tctgcgatat ttttctcttg
    14281 atagtaaact tctaacgtct ctttgttgta acgtttgcca tgacagacat cacactcgac
    14341 ataaacgtca ggcaaaaatt gcatttcaat tttaatttgg ccctctcctt gacaagtttc
    14401 acaacgtccg cctttgacat tgaaactgaa acggccggtt ttgtaggcgc gaatttgtgc
    14461 atctctagta ttagcgaaaa cttgccgaat aagatcaaag gttttagtgt
//
LOCUS       DAOSEW010000005        14388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_3472_length_14388_cov_5.089025, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000005 DAOSEW010000000
VERSION     DAOSEW010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 14388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14388
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3472_length_14388_cov_5.089025"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PLJ02_00390"
     rRNA            <1..372
                     /locus_tag="PLJ02_00390"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            488..603
                     /gene="rrf"
                     /locus_tag="PLJ02_00395"
     rRNA            488..603
                     /gene="rrf"
                     /locus_tag="PLJ02_00395"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(641..865)
                     /locus_tag="PLJ02_00400"
     CDS             complement(641..865)
                     /locus_tag="PLJ02_00400"
                     /inference="COORDINATES: protein motif:HMM:NF040135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04919.1"
                     /translation="MPELTIDQNFISTLLKVFFVIGASFYLIYSGVVVKQIIVMKKTL
                     ITGFSSLITLFGLINLIIATVMLLAFILFL"
     gene            complement(865..1485)
                     /locus_tag="PLJ02_00405"
     CDS             complement(865..1485)
                     /locus_tag="PLJ02_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04920.1"
                     /translation="MKQKRQVEDYSEVMKHETATDNPLKAFIPKPNKITFEIQDRDEK
                     IILVLRQHPITQVKNLFILLFSFLVIPWLLKTAGILAFFPNQFRLAFNVFLVVLFLGM
                     ALRYFLLWFFNVYIITDERIIDVDFLSMIYKNISTAKIENIEDITKQTSGIMASVFDY
                     GTILIQTAATKNEFEFEHVPHPATVVKLLNELVLEEEREKIEGRVN"
     gene            1655..2470
                     /locus_tag="PLJ02_00410"
     CDS             1655..2470
                     /locus_tag="PLJ02_00410"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOI04921.1"
                     /translation="MPAKKTKKTPSQASTKKSVGKKVTTKKTVSTVNASKAPAKKPLK
                     AKLLSSNAATKKIAVNKTEQVVNQEKPVKKEQLAKKSIWPIIILLVALALWFLRSQII
                     VAMVNGKPITRFKLISELEKQGASQILDSLVTMELVNQAINEADIKVDDQEIADQMAE
                     IEATLASQNQNLDDILAMQNLTREDIEKDIKLNLQVDKILADKIQVTDEEVMEYFETN
                     KEILGEDANFEEMKKDIEAQVIQEKRSAAQQEWLETLRSEANIRYLKFEPSLL"
     gene            complement(2467..4287)
                     /gene="lepA"
                     /locus_tag="PLJ02_00415"
     CDS             complement(2467..4287)
                     /gene="lepA"
                     /locus_tag="PLJ02_00415"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOI04922.1"
                     /translation="MAELSKIRNFSIIAHVDHGKTTLTDVFLHLTNAVSKSKFHERVM
                     DSNPIEQEKGVTIKLAPVRMNYKGYILNLIDTPGHVDFAYEVSRSLAACEGAVILVDA
                     SQGVQAQTLANYQKAKDLGLTIIPVINKIDLPSANVEQCLLEMMEIFNLKEEEILQVS
                     AKTGQNVEKVLDAIIDRIPPPQTTLIQERQAKAINTSDEKKATQALIITSKFDNHQGA
                     IAYVRVVSGQINKESLHLISTNISFNPIEIGVFTPDPTKKEFLTTGEVGYIATGLKDT
                     SQLKIGDTITTVREKKIAKPLAGYQEPQAMVFMELYPIDSKNFTELKEAMAKLQLHDS
                     ALQYQTTNSVALGNGLRVGFLGIFHAEIVRERLSREFDLELISTAPSITYQIVMDNQE
                     NLLIHNPAELPEISRIKEIREPICQLNIFSPEEYLPSIMKFCQERRGQLKNSLRAGNR
                     NQLQYEMPFAELVNDFHDQLKSISSGFASMDYRFSHYQTVDAVKVDILINLVKIEALS
                     FITTRDNADKKARAIVKKLKEVIPRHMFEIPIQAAIGGKIIARETIKAFRKDVTAKLY
                     GGDRTRRMKLLKKQSKGKKRMKQFGKVELNQEAFLAVLEN"
     gene            complement(4287..4529)
                     /gene="rpsT"
                     /locus_tag="PLJ02_00420"
     CDS             complement(4287..4529)
                     /gene="rpsT"
                     /locus_tag="PLJ02_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012447634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOI04923.1"
                     /translation="MPILKNAKKALRVSERKNIVNRKVKSQVKTAIDQAIKEPSQENL
                     AKASSKIDRAVKKNLIHKNKAARVKSRLAKLKSKSS"
     gene            4706..6352
                     /gene="murJ"
                     /locus_tag="PLJ02_00425"
     CDS             4706..6352
                     /gene="murJ"
                     /locus_tag="PLJ02_00425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOI04924.1"
                     /translation="MAKKQNSILSAAFIITVANISSSLLGLLRERVLIKLFFNNIEGQ
                     QAYEAFQVAFQIPDLLFQLIVLGALSAAFIPLFTELKRKDKKKAFTFTSTVMNLVLLI
                     FAFFSIVAFIFARPITLARTGAEFTPEQVEIAVNLTRVMLFAQFFFAISNFLTGILQA
                     YQRFIIPAIAPLVYNLGIIITSIFLAQYLGIYAAGLGVVIGAALHMLIQLPLILKTGY
                     RWQASFDWRFSEVKKLFKMMPPRLMTVGVGELQNLAMGFFATSIGNLSFVIINLARRL
                     MTLPIRFFGVPISQASFPFLSEESADANRKKFNQLLLQSLNQISFLAMPASVLLLILR
                     VPAVRLVFGTANFPWQTTIATGKVVALIAISITAQAMVQLLIRGFYALKDTRTPFMVT
                     TISVLVYLMTNVVGIYVFNLGVFSLGISITVAAFVELLLMLILLQKKIQCFTFADFFL
                     PQLKILLASFFMAVFLYLPFKILDEVVFDTTRTVDLIALTVSTSTIALLVYLYFSILF
                     DVKELSYFNSLIAKFGKWRPVLEDSQEVLLDSSIEDELSK"
     gene            6407..6997
                     /locus_tag="PLJ02_00430"
     CDS             6407..6997
                     /locus_tag="PLJ02_00430"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HOI04925.1"
                     /translation="MPSSKSKKKKQTPVVLDVNLVPKDPFLETAIGRSLKWTVNVGRY
                     IVIFTQLVVIVSFLTRFFLDRQVTDLNVTINQQKNAIEAYHDLEENFLFVQAQIADVK
                     QLQQESNLIEIFPILNETIPDNVILDELTIKPNEVLFTGIALSQEALNVLVNNVKLSP
                     YFTDIAMDKIESRGENLPGLTFDMRAKVVKTAATTE"
     gene            7012..7650
                     /gene="pilO"
                     /locus_tag="PLJ02_00435"
     CDS             7012..7650
                     /gene="pilO"
                     /locus_tag="PLJ02_00435"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HOI04926.1"
                     /translation="MAKTDQAQLTAVLMDFYQKPIARVSIELILSVLIVILFAVFAIR
                     PTLITMAELVKEIEDKKELSEQMNLKLASLATAQEQYTLHQSQFYLLEEAIPRRLDVV
                     KSLKKLEKLAGEEQLVINTMSISTMPGAVKEESLTNNFKDYQREFLPINIEVIGSYLQ
                     IREFVEKIMNIRQVIIVDQVVVERMTETDNLSATLRVSLPYYVLSSTVDQNE"
     gene            7643..8032
                     /locus_tag="PLJ02_00440"
     CDS             7643..8032
                     /locus_tag="PLJ02_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04927.1"
                     /translation="MNKERARLKQELNRLRRQKSILWAGILFLVAVLIWTAVSILTSQ
                     KQVKIDQQLTELAKPLIPRLDVEVFSMIEEKRAITDEELSIFPIYVYLVSGDEKRGEI
                     GVFTDISNLATNSAMLDQEVLVEEASF"
     gene            8670..10154
                     /locus_tag="PLJ02_00445"
     CDS             8670..10154
                     /locus_tag="PLJ02_00445"
                     /inference="COORDINATES: protein motif:HMM:NF027967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOI04928.1"
                     /translation="MSLVIGVIFFGNGTGLFAPRATPETTPKNITNSNITNKSFTVSF
                     YTDEETIAFIKYGTSPNDIKKQVSDDRDQLSGVIKPYRLHHITVKGLEPNTTYYYLLG
                     TGSNTTFDNGGNPFQIKTASGPGGVSPNNQTIYGTVSQANGQPGEGAIVFVTVEGAST
                     LSTLVKSSGSWAVSLANAFNLSLNGYPEVNEDSVLSIKVQGIEPNSITSRVTNVTSAQ
                     PVPDIIMGQEAASTDDLSATREELLGESNQDTGKNSEDTEEIELEESTTENSLIAQES
                     TESTAINQNVESQDSSVIGELNEQDRTLDLTSVGEEEDIVINSTQPIIKTNLPPNVTV
                     KIEVNSDTAIETVKATDANGELVLDIASLEQSLSPGEHTVTYTYVDPNTGEEVRESYD
                     FRVSASDDVQQAEEETSTIAAADTTTLPYGSGNPYVPSITPSVSPTPTPIATESARET
                     VVSTESGTYNAGSVFETMSLLMVGLFFLATGVWSFLLAKQFKTS"
     gene            complement(10189..11325)
                     /locus_tag="PLJ02_00450"
     CDS             complement(10189..11325)
                     /locus_tag="PLJ02_00450"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOI04929.1"
                     /translation="MTNKLWPKKSNQEMIVYGFLPYWNIDKVNIQPELTHLSYFALEV
                     GTDGQIVTESQPGETEPGYRELNSDQFFNLINDHHLNLEIVLSQFDAKKIEQLLSDQT
                     AWLTLLEQIDSLLLAYPFSGINLDFEYQGEANELLRQQFTNLVISLHNHLAQHQEKIN
                     LSVDVYASAGDPAKKLLWNIPALEPYLDYVVVMAYDFHRQSSTQAGPGAPLFGAGGLW
                     NSDINSLMKGFFEQVPKEKILLGVPFYGYAWQTDSLKPNANTYPGTGFTVSYEEVLNL
                     KQLSGIWQNCTNLEIFWQEDALSPFASLSCGVKEKEDQEPTDTATEKSEKKINYLVYF
                     EDEKSLQYKIDFANQLEVAGIAIWALGYEGETRDLWEVVGKLRN"
     gene            complement(11472..13817)
                     /locus_tag="PLJ02_00455"
     CDS             complement(11472..13817)
                     /locus_tag="PLJ02_00455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOI04930.1"
                     /translation="MLNKTHTQKMNWSKFWRNQKWHVILIVLAIAVIGTLIYLISELP
                     SPKKLSSSEYYAVSTQIFDRNGELLYEVYGDENRTPVKIETLPEHLLQATIAIEDKNF
                     NRHHGLDVRSLLRATINNLTGGSTQGGSTITQQLVKNALLTKERTLNRKIKEAILSVL
                     AEIIYSKEEILEMYLNYIAYGGTAVGIEAAANQYFNKSASELTLAESSLLAGLPKAPS
                     TYSPFGAHPEKAKQRQKEVLRRMFEDGYINEEQMNEVANETLTYALKKTDIKAPHFVF
                     YVIDLLQNQYGEEMVKTGGLRVTTTLDLELQEAAQASLSAEIADLANLKVSNGAALIT
                     KPNTGEILAMIGSRNYFDSENDGQVNVTLALRQPGSSIKPLMYATTFQEKTLNPGSML
                     LDIPTCFRVAGQKDYCPKNYTGGFAGPVTVRQALGNSLNIPAVKALSTIGVEKFIEQA
                     NKMGISSWTDPSQYGLSLTLGGGEVTMLEMATAFGVLANQGVKVPSTAILEIKNYQGE
                     TLESVNLVARGETLSAMTEDETIEQANDLERVMRRAPAYLTSHIMQDNNARLMAFGSN
                     NQLVIKDKVVSAKTGTTNDLKDNWTVGFTPEFLTIVWVGNNDNTSMSYLASGVTGAAP
                     IWNDIMSYVLENEQVIWPERPRDVKMGYVCQTGMPVDRGLGNNRNDLQNRNISDLTVK
                     MDDQQTYLTETDPIQTCQASSQELYWEESLPSYSGSFQKEFWIRSETGLPPAVGEEAN
                     DLVLELHQFYYDPVTALYCADCRRPVDEETGETIYEKTYVN"
     gene            13886..>14388
                     /locus_tag="PLJ02_00460"
     CDS             13886..>14388
                     /locus_tag="PLJ02_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04931.1"
                     /translation="MQFDSQAPWLKYLDPSQRELVEVSFYLSQEFANNTTLQDFSFIV
                     FPMAKAYEGFLKKVLLDLNLINQEVYYGRHFRIGRALNPDVSPNQRDQWWLFDDLSRM
                     LNHDLAQEIWQTWLQCRNHVFHFFPNGEQKLNFAEAVNCLNQMQNVMKTTITLIEERH
                     EKKVKQA"
BASE COUNT         4386 a         2614 c         2703 g         4685 t
ORIGIN      
        1 catcctgggg gtgaagaagc tcccaagggt ttggctgttc gccaattaaa gcggtacgcg
       61 agctgggttc agaccgtcgt gagacaggtc ggcctctatc cggtgttagc gttagaaagt
      121 tgagggaatt gatctctagt acgagaggat cgagatgagg gaatccctag tgttcctgtt
      181 gttgctgtca agcgcaccgc agggtagcta catttcttaa ggataatcgc tgaaagcatc
      241 taagcgagaa gccccaccca agattaactt tctccattag acccgtggaa gactaccacg
      301 ttgataggtt gccggtgtaa gtatggtgac atattaagcc tagcaatact aagtgtcgat
      361 tgaataaatt acaaatcagt tttcgttgtt tctcaatgac tattattggg cgacagttga
      421 cttcttcaca tgttaaagca cagttgtgct gtataattaa aagactaaat ttaaaaaaac
      481 cttttgttct ggtctatgga gcgatatggc tccacctctt cccatcccga acagagtctt
      541 gaaacgtctc agcgctgaca atagttaggg gtttcccctt gcgaagatag gtcgaggcca
      601 gaacaaaggg ttttttatat tctaggcaat cttttagctt ttagagaaaa agaataaaag
      661 ccaagagcat cacagtggca ataattaggt taatcaagcc gaacaaggtg attaagctgc
      721 taaagccagt aattaaagtt tttttcatga caataatctg tttcaccacc actccagagt
      781 agataagata aaaggaagct ccaataacaa aaaaaacttt aagcaaagtg gagataaaat
      841 tctgatcaat tgttaattct ggcactaatt gaccctccct tcaatttttt ctcgttcttc
      901 ttctaacacc aattcattta atagcttaac cacggtagca ggatggggta cgtgttcgaa
      961 ttcaaattca tttttagtgg cagcggtttg aattaaaatt gtaccgtagt caaaaactga
     1021 ggccatgatg cctgaggttt gtttagtaat atcttcaata ttttcaattt tagcagtcga
     1081 aatgttctta taaatcattg ataagaaatc aacatcgata atccgttcgt ctgtaataat
     1141 ataaacatta aaaaaccaca gcaaaaaata acgaagggcc atgccaagaa aaaggactac
     1201 tagaaaaaca ttgaaagcca atcgaaattg atttggaaaa aaagcgagta ttccagcagt
     1261 ttttagtaac caaggtataa ctaagaatga aaaaagtaga ataaaaaggt ttttcacctg
     1321 ggtaattggg tgttgccgca aaactaaaat aattttttca tcacggtctt gaatctcaaa
     1381 ggtaatttta ttgggtttag ggataaaagc ttttagtgga ttatccgttg cagtttcatg
     1441 tttcatgact tctgaataat cttcgacttg tcttttctgc ttcattggtt tttccaaagc
     1501 taattatatc aagaatcatt atacattaaa attcttaatt ctgagttttt cccctagtat
     1561 tttcttaaaa aaaatgataa ctttgataca gtctgatttt ttattgatag ataaactaga
     1621 caagtcagat tttttaatta agagaaaggt tgatatgcca gctaaaaaaa caaaaaagac
     1681 cccgtctcag gcaagcacaa agaagtccgt gggaaaaaaa gtcacaacta aaaaaactgt
     1741 tagtactgtt aatgctagta aagcgccagc aaaaaaacca ctcaaagcta aacttttgag
     1801 tagtaacgct gctactaaaa aaatcgctgt taacaaaact gaacaagtgg ttaatcagga
     1861 aaagccggta aaaaaagaac aactcgctaa aaaaagtatt tggccaatca ttatattatt
     1921 agttgcccta gccttatggt ttttgcggag tcaaataatt gtggccatgg taaatggtaa
     1981 acccattact cgttttaagc ttattagtga attagaaaaa cagggagcta gccaaatttt
     2041 agattctcta gtgactatgg aattggttaa tcaggctatt aacgaagcag atataaaagt
     2101 tgatgatcaa gaaattgctg atcaaatggc cgaaatagaa gcaactttgg cttcccaaaa
     2161 ccaaaatcta gatgatatct tggcgatgca gaatttaacc agagaagata tagaaaagga
     2221 tatcaaactt aatttacaag ttgataaaat tttagctgat aaaatccaag ttactgacga
     2281 agaggtaatg gaatattttg aaactaataa ggaaattttg ggagaagatg ctaattttga
     2341 ggaaatgaag aaggatatcg aagctcaagt aattcaagaa aaacgcagcg ctgctcaaca
     2401 agagtggttg gagactttgc gtagtgaagc taatattcgt tatctcaaat tcgaaccaag
     2461 cttgctttag ttttctaaaa cagctaaaaa ggcttcttga ttcagttcga ctttgccgaa
     2521 ctgtttcatt ctctttttac ctttactctg tttcttgagg agtttcattc ttcgagtacg
     2581 atcgccacca taaagtttag cggtgacatc tttgcggaag gccttaatag tttccctagc
     2641 aatgatttta ccgccaattg ccgcttggat tggtatttca aacatatgtc taggtatcac
     2701 ttctttaagt tttttcacaa ttgctcgcgc ttttttgtcg gcattgtctc tagtagtaat
     2761 aaagctgagg gcttcaattt tgactaaatt aattaaaata tcaactttga ctgcgtctac
     2821 agtttggtaa tggctaaagc gataatccat cgaagcgaaa ccagaactaa tcgattttag
     2881 ttgatcgtga aaatcgttta ccaattccgc gaatggcatc tcgtattgca gctgattgcg
     2941 attcccagca cgtaaactat tttttaactg tcctcgtctt tcttgacaaa atttcataat
     3001 tgaaggaagg tattcctctg ggctaaaaat atttaactga caaataggtt ctctaatttc
     3061 cttaatgcga ctaatttcag gcaattcagc gggattgtga atcagcaagt tttcttgatt
     3121 gtccatcaca atctggtagg taatcgaagg agcagtacta attagttcta aatcaaactc
     3181 tcttgatagc ctttctctaa ctatttcagc atggaaaatc cccaaaaacc cgacgcgtag
     3241 tccattgcca agtgctacag aattagtggt ttgatattgt aaagcactgt catgtaattg
     3301 caatttggcc attgcttctt tgagctcagt aaaatttttg ctatcgattg gataaagttc
     3361 cataaaaacc atagcttgtg gttcttgata accagccaat ggtttagcga ttttcttttc
     3421 tctaacggtg gtgatagtgt cgccgatctt caattgacta gtgtctttca gtccggtggc
     3481 aatatatcct acttcacctg tggttaaaaa ttccttttta gttggatcag gggtaaaaac
     3541 gccaatttcg attggattaa agctaatatt agtcgaaatc aaatgtaaac tctctttatt
     3601 aatttggcca ctcactactc tcacataagc aatggcccct tgatgattat caaattttga
     3661 agtaataatt agggcctgag tagctttttt ctcgtcacta gtattaatag cttttgcttg
     3721 tctttcttga attagggtag tttgtggagg tggaattctg tcgatgatgg cgtccaaaac
     3781 tttttcgacg ttttgtcctg ttttagcact cacttgtaag atttcctctt cttttaaatt
     3841 aaaaatttcc atcatctcta atagacactg ttcgacattg gcagagggca aatcaatttt
     3901 attaatgacc gggataatgg ttaaaccaag atctttagcc ttttgataat tagctaaagt
     3961 ttgggcctgt actccctgac tggcgtcaac taagattacc gccccttcgc aagctgctaa
     4021 ggaacgactg acttcataag caaaatcaac atgtcctggc gtatcaatca aatttagaat
     4081 atatccttta taattcatgc gtactggagc caatttgatg gttactcctt tttcttgttc
     4141 aatggggttg gagtccatca ctcgttcgtg aaactttgat ttgctgacag cattagttaa
     4201 atgcaaaaaa acatcggtca aagtagtttt gccgtgatcg acatgtgcaa tgatagaaaa
     4261 gtttctgatt ttcgagagct ctgccattat gacgatttgc ttttgagctt ggcgagtcta
     4321 gatttgacgc gggccgcttt atttttgtga attaaatttt tcttaacagc ccgatcaatt
     4381 ttactactag ccttagctaa attttcttga cttggttctt tgattgcttg atctatggca
     4441 gttttaactt gagattttac tttgcgatta acaatatttt ttctctcgct aactcttagg
     4501 gcttttttag cattttttaa aattggcatt gatttccttc tttcaattta acaaggcgtg
     4561 attatacaag aaaagaatcg atcatacaag tttccctggt tggtgctaat taaatcttag
     4621 atttcctttt tttctgattt tttcttataa ttaagctact catatgattt catttaataa
     4681 aatttttaaa gctaattctt actggatggc aaaaaaacaa aattcgattt tatcagccgc
     4741 ttttattatt acagtggcca atatttcttc ttcgttatta ggtttgctga gagaaagggt
     4801 cttaattaag ctctttttta ataatattga gggtcagcag gcctacgagg cttttcaagt
     4861 cgcttttcaa attccagatt tactttttca actcatcgtt cttggagcat tgtccgctgc
     4921 ctttattcca ctttttacgg aattaaaaag gaaggataag aagaaagctt ttacattcac
     4981 ttcaacagtt atgaatttgg tattattaat ttttgctttt ttctcaattg tcgcttttat
     5041 ctttgctcga ccaattactt tggctcggac aggggctgaa tttactccag aacaggtaga
     5101 aattgccgtc aatttgacta gagtcatgtt atttgctcag ttcttctttg ctatttccaa
     5161 ttttttaaca ggaattttac aggcttatca acgctttatt attccggcca tagctccctt
     5221 agtttataat ctggggatta ttatcacctc catcttttta gctcaatatt tgggtattta
     5281 tgccgctgga ctcggtgtgg tcattggggc agcgctacac atgctcattc aattaccttt
     5341 aattttaaaa actggttatc gctggcaagc ttcatttgat tggcgcttta gcgaagtaaa
     5401 aaaactattc aaaatgatgc cccctcgctt aatgacagtc ggagtaggag aactacaaaa
     5461 tttggctatg ggattttttg ctactagtat tggcaattta agtttcgtga ttatcaatct
     5521 agcaagacga ttaatgactt tgccgattcg gttttttggg gtcccaatta gtcaagcttc
     5581 ttttccgttt ttatcagagg agtcggctga tgcaaacaga aaaaagttta atcaattact
     5641 gttgcagtca cttaaccaga tttccttttt agctatgcca gcttcagtgc tattgttgat
     5701 tttgcgagta ccagcagtac gtttggtttt tggtacggct aactttcctt ggcaaaccac
     5761 gattgcaacg gggaaagtag tagcattgat tgctatttca atcactgccc aagcgatggt
     5821 gcagttatta attcgtggtt tttacgctct caaagacacg agaacgcctt tcatggtgac
     5881 tactatttca gtacttgtat atttaatgac caatgttgtg ggaatttatg tttttaacct
     5941 gggcgtgttc agtttaggca tatcaattac tgtagcggct tttgttgaac tattgttgat
     6001 gttgatttta ttacaaaaga aaattcaatg ttttaccttt gcggatttct ttctgcctca
     6061 gctaaaaatc ttgttggcca gtttctttat ggctgttttc ttatatttgc cctttaagat
     6121 cttggatgaa gtagtttttg atacgacgag aacagttgat ctaattgcct taactgtttc
     6181 caccagtacc attgctttat tggtctatct ttatttttcc attttatttg atgtgaaaga
     6241 attgagttac tttaatagtt taattgctaa atttggtaag tggcgtccag tactcgagga
     6301 ttctcaagaa gtactgcttg atagttctat tgaagacgaa ttatctaaat aatttttttc
     6361 tgataagctc ttttattctc tataaaatta atttacaatg tagataatgc ccagtagcaa
     6421 gtctaagaaa aaaaaacaaa ctccggttgt attagatgtc aaccttgtcc ccaaagatcc
     6481 attccttgag acagctatcg gtcggtcgtt aaaatggacg gttaatgttg gtcgttacat
     6541 agtaattttt actcaattag tagtgattgt gagttttttg acccgtttct ttttagatcg
     6601 tcaagtaaca gacttaaatg tgactattaa tcagcaaaaa aatgctatag aagcttatca
     6661 cgatttggaa gagaatttcc tttttgtaca agctcaaatt gctgatgtca aacagttaca
     6721 acaagaatca aatttaatcg aaatttttcc tattttaaat gaaactattc ccgataatgt
     6781 aattttggat gaattgacga ttaaaccaaa tgaagttctt tttacaggta ttgcattatc
     6841 tcaggaagcc ttaaatgtgt tggttaataa tgtcaaactt tctccttatt ttactgatat
     6901 tgctatggat aagattgaat ccagaggaga aaacttacct ggtttaacct ttgatatgag
     6961 agctaaagtg gttaaaacag cagcaactac tgaataagta attggataat aatggcaaaa
     7021 acggatcaag cacaactaac agcagtttta atggactttt atcaaaagcc tattgccaga
     7081 gtttctatag aacttatttt gtcagtgcta attgtcattc tttttgctgt ttttgccatt
     7141 cgtccgactc taatcaccat ggctgagttg gttaaagaaa ttgaagataa aaaggaattg
     7201 agcgagcaaa tgaacctgaa attagctagt ttggctactg cacaggaaca atataccctg
     7261 catcaatctc aattttattt actggaagaa gcgattcctc gacgattgga tgtggtcaaa
     7321 tcattaaaaa aattggaaaa actagctgga gaagaacaac ttgtgattaa tacaatgtcg
     7381 atatccacaa tgccaggagc agttaaagaa gaatcactga ctaataattt taaagattat
     7441 caaagagagt ttttaccgat taatattgaa gtgattggta gttacttgca gattagagag
     7501 tttgtagaaa aaatcatgaa tatccgtcaa gtaattatag tagatcaggt agtagtagaa
     7561 agaatgacgg aaacagataa tttatctgcc acgttaagag tttccttacc ttattacgtg
     7621 ttaagttcaa cagtggatca aaatgaataa agaaagagcc agactaaaac aagagcttaa
     7681 ccgattacgg cgacaaaaaa gcattttgtg ggctggaata ttatttttgg tagcggtatt
     7741 aatctggacg gctgtcagta tattaacttc tcaaaaacaa gttaaaattg atcagcagtt
     7801 gactgagttg gctaaaccac tgatacctcg tctcgacgtt gaagtttttt ctatgattga
     7861 agaaaaacgg gccataaccg atgaagagct gagtattttt ccaatttatg tttatttagt
     7921 cagtggtgat gaaaaacgag gtgaaatagg cgtgtttact gatattagca acttagctac
     7981 caatagtgcc atgctcgatc aagaagtgtt agtggaagaa gcttcttttt aatttactcg
     8041 ctaattaata tcaaaaaata attgatcatt caataaattt attaaaatga ttggtttttt
     8101 gtttggtttt ttttcgcctt gtgatctatt tttaattcgt ataaagtaat attaaggcac
     8161 tttttgtgtc ttggttttga tatgcaagtt gtaaataata atcaatcagc ggctactaaa
     8221 cttagtcaac atttgcctaa gccagtgccc ccaccaattg taactgctca aacaaacact
     8281 cctgtaaaaa atccggctcc aatgatggag aatcaaaaat ttgcttcacc aatagcccaa
     8341 gtttcaaacc ggcagtagca ggcgcacctg caccacaacc aataaccaat acatctgttc
     8401 aacggccaat tgggagtact atcggccaaa aaccagtcag tccagttgca aataatctgg
     8461 gcgcatccag ccaatctagc atctatccag gtcggtcctt accaacaact aatgatcaaa
     8521 aactagtacc aaccgagcaa attggaatta gaaaagaagg caaaattgct aaaaaaggaa
     8581 aaaagcaaaa agataaatca gaaaagaacg taaagaagag ctctggtaaa aaagaaatac
     8641 caaccatctt agggatatta cttttagttg tgtcgttggt gattggtgtt attttctttg
     8701 gtaatggaac tggtctattt gctcctcgag caactccaga aacgactccc aagaatatta
     8761 ccaatagcaa tattaccaat aagtcattta ctgtctcttt ctatactgac gaagaaacaa
     8821 tcgcttttat caaatatggc actagcccta acgatataaa aaaacaagtc agtgatgaca
     8881 gagatcagtt gtcgggagta attaaacctt accgccttca tcatattact gttaagggtc
     8941 tagaacctaa tactacctat tattatctgc ttggcactgg tagtaataca acttttgata
     9001 atggaggaaa tccctttcag atcaaaacag ctagcggccc cggtggtgtc tccccgaaca
     9061 atcaaactat ttacggtacg gtgagtcaag cgaatggaca gcctggtgaa ggagcgattg
     9121 tttttgtgac tgtagaagga gctagcactt tatctacctt agtcaaatct tcagggagtt
     9181 gggcagtttc tttggccaat gcttttaatt tgagtttgaa tggttacccg gaagtaaatg
     9241 aagacagtgt gctatcaatc aaggtacaag gtattgaacc aaattcaatt actagtagag
     9301 tcactaatgt aactagtgct caaccggtgc cagatattat tatgggacaa gaagccgctt
     9361 ctacggatga tttgtctgcc actagagaag aattactagg tgaaagtaat caagacactg
     9421 gaaaaaatag cgaagatact gaggaaattg aattggaaga aagtactaca gaaaattctt
     9481 taattgctca agagtctaca gagagtaccg caattaatca aaatgtagaa agtcaagatt
     9541 cttctgtaat tggagagcta aacgaacaag atcggacttt agatttgacg agtgttgggg
     9601 aagaagaaga cattgtcatt aactccactc aaccaatcat taaaactaat ttaccaccta
     9661 atgttaccgt taaaatcgaa gttaattctg atacagcaat agaaacagtg aaagcaacag
     9721 atgctaatgg tgaattagtg ttagacattg ccagtctcga acaaagttta agtccaggtg
     9781 aacataccgt aacttacaca tacgtagatc caaacactgg agaagaagtt cgggagagtt
     9841 atgattttag agtaagtgct agtgatgatg ttcagcaagc agaggaggaa accagcacta
     9901 ttgccgctgc tgatacaaca actttaccat atggcagtgg taacccctat gttccgtcaa
     9961 ttacgccgag tgtaagccca actcctactc ccatcgctac cgaaagcgct agagagacag
    10021 tagtttccac ggaaagtggc acttacaatg ctggttcggt atttgaaacc atgagcttat
    10081 taatggttgg tttgtttttc ttagccactg gagtttggtc atttctttta gccaaacaat
    10141 ttaagaccag ttaatgaatt caaatcgaat gagttttaag cagataaatt aatttcttaa
    10201 ttttcctacc acttcccata gatctcgagt ttctccctca tatccaagag cccaaatagc
    10261 aatgccagcc acttctagtt gattagcaaa atcaattttg tattgtagcg atttttcatc
    10321 ttcaaaataa actaaataat ttattttttt ctcgcttttt tcagtggcgg tatcggttgg
    10381 ttcttgatct tctttttctt tcaccccgca gcttagactg gcaaagggag aaagggcatc
    10441 ttcttgccaa aaaatttcta aatttgtgca attctgccaa ataccactta attgtttgag
    10501 gttaagtacc tcctcataac taacagtgaa gccggtgcct gggtaagtat tggcgtttgg
    10561 ttttaaacta tcggtttgcc aagcatatcc ataaaacggc acacctaata aaattttttc
    10621 ttttggtact tgttcaaaaa aacctttcat tagtgagtta atatcactat tccatagccc
    10681 tcccgcgcca aacaagggtg cgcccggccc tgcttgagtt gagctttggc gatgaaaatc
    10741 gtaggccatt accacaacat aatctagata cggttccaga gctggaatat tccacagcag
    10801 tttttttgca ggatctccag cactagcata aacatcaaca cttaaattga ttttttcttg
    10861 atgctgggct agatgattat gaagactgat taccaagttg gtaaattgtt gacgtaacaa
    10921 ttcattagct tcaccctgat attcaaaatc taagttgatc ccgctgaatg gataagcaag
    10981 taataaactg tcaatttgct ctaatagagt taaccaagct gtttggtcac ttaggagctg
    11041 ttcaattttt ttggcatcaa attggcttaa aactatttct aagtttagat gatgatcgtt
    11101 gattaagtta aaaaattgat cactatttag ttctcgataa ccaggttcag tttcacctgg
    11161 ttgactctct gttactattt gaccatcagt acccacttct aaggcaaaat aacttaaatg
    11221 ggttaactcg ggttggatgt taactttgtc aatattccaa taaggtaaaa aaccataaac
    11281 aatcatttcc tgattggact tttttggcca cagtttgtta gtcatatagt tgaattgaaa
    11341 attttcgccg attggcgact taaaattttt ttgccaaatt ttgacagcaa aatagccaat
    11401 taaaagcaaa aatataacaa ggaggaaaaa cacaatgtat ttattttttt tacgcacttt
    11461 tagattaagc attaattaac gtaggttttt tcgtaaatag tttctccagt ctcttcatct
    11521 actggtcgtc ggcaatctgc acaatagaga gcagtaactg gatcataata gaattgatgt
    11581 aattctaaaa ccaaatcatt tgcttcttcg ccaactgctg gtggtaggcc agtttcactg
    11641 cgaatccaaa attctttttg aaaactgccc gaataacttg gtaaagattc ttcccaatac
    11701 aattcctgac tgcttgcttg acaggtttga attggatcag tttcggttaa ataagtttgt
    11761 tggtcatcca ttttcaccgt caaatcactt atgtttctat tttgtaaatc attacgatta
    11821 ttacctaagc cacgatcgac cggcatccca gtttgacaaa catagcccat cttaacatca
    11881 cgtggacgct ctggccaaat aacttgttca ttttctaaaa catagctcat gatatcattc
    11941 caaattggtg cggcaccagt gacacctgaa gcaaggtagc tcatggaagt attgtcgttg
    12001 ttgcccaccc aaacaatggt gagaaattct ggggtaaagc ccactgtcca gttatctttc
    12061 aagtcattag tcgtgccagt tttggcactg actaccttgt ctttgatgac cagttggttg
    12121 ttactgccaa aagccattaa gcgggcatta ttgtcttgca tgatgtgact agttaaatag
    12181 gctggcgcgc ggcgcatcac tctttccaaa tcgtttgctt gctcaatagt ttcgtcttcg
    12241 gtcatagctg acaaggtctc gccgcgcgcc accaaattaa cactttctag agtctcacct
    12301 tgatagtttt ttatctctaa gatagctgta ctgggcactt ttactccttg attggctaag
    12361 acgccaaaag cggtggccat ttccaacatg gttacttcgc ccccgcccag agttaatgac
    12421 aagccatatt gactgggatc ggtccagcta ctaataccca ttttgttggc ttgttcaata
    12481 aatttttcca caccaattgt gcttaaagct ttgactgctg gaatatttaa tgagttgccc
    12541 agcgcttgtc taacagtaac tggtccagcg aaaccgccgg tataattttt tggacaataa
    12601 tccttctgtc cagccacgcg aaaacatgtg ggaatatcta gaagcattga gccgggatta
    12661 agggttttct cttgaaaagt ggtggcatac atcagtggtt taatcgaaga gccaggttgt
    12721 cttaatgcca aagtcacatt aacttgtcca tcattttcac tatcaaaata attacggctc
    12781 ccgatcattg ctagaatttc tcctgtgtta ggtttagtga taagagcagc cccattactg
    12841 acttttaagt tagccaaatc ggcgatttct gcagaaaggg aagcttgcgc tgcttcttgc
    12901 aattccaaat ccaaagttgt tgtgacgcgc aacccgcccg tttttaccat ctcttcacca
    12961 tattgatttt gtaataaatc aatgacataa aaaacaaaat gtggtgcttt gatatcggtt
    13021 ttttttaggg cataagttaa tgtttcattt gcaacttcat tcatttgttc ttcattgatg
    13081 tatccgtctt caaacattcg gcgtaaaacc tctttttgtc gttgtttagc cttttctgga
    13141 tgggcgccaa aaggtgaata agtactaggc gctttgggta aaccagctaa cagcgaactt
    13201 tcggccaaag ttaattcgct ggcactttta ttaaaatatt gattagctgc agcttcgatg
    13261 ccgacggccg taccaccata agcaatataa ttgagataca tttctaatat ttcttctttg
    13321 ctataaataa tttcagccaa aactgacaaa atcgcttctt taattttgcg attgagtgtt
    13381 ctttccttag ttaataaagc atttttcact aattgttgag taatagtaga accaccctgg
    13441 gtactaccac cagttaagtt attgattgtc gctcttagca aacttcgcac atccaaacca
    13501 tgatggcggt taaaattttt atcttcaata gcgattgtgg cttggagtaa gtgttcaggc
    13561 agtgtttcaa ttttaactgg tgtacgattt tcatcgccat acacttcgta aagcagctct
    13621 ccattccgat caaaaatttg cgtgctaacg gcgtagtatt ctgaagaact taattttttg
    13681 ggagatggca gttcggaaat taaataaatc aacgtgccaa ttactgcaat agccaaaaca
    13741 attaaaatga catgccattt ttgatttcgc caaaattttg accaattcat tttttgcgtg
    13801 tgtgttttat tgagcatagg tcttggattt tttcttgtgt ttcaattata atcgatatgt
    13861 atttttctgt aagatgaact tatttatgca atttgattca caagctccct ggttaaaata
    13921 tttggatcca agtcaaaggg aattggtcga agttagtttt tatctcagtc aagagtttgc
    13981 gaacaacacc actctccaag atttttcttt tattgttttt cccatggcca aagcttatga
    14041 aggttttctg aaaaaagttt tactcgatct aaatttaatt aatcaagagg tttattatgg
    14101 tcgtcatttt cggattggtc gggctcttaa tccagatgtg agtcctaatc agcgtgatca
    14161 atggtggctt tttgatgatc taagtcgcat gttaaatcat gatttagctc aagaaatttg
    14221 gcaaacttgg ctgcagtgtc gcaatcatgt ctttcatttt ttccctaatg gtgaacaaaa
    14281 attaaatttt gcagaagctg tgaattgtct caatcaaatg caaaatgtga tgaagacaac
    14341 catcacatta atcgaagagc gtcatgaaaa aaaagttaag caagcaaa
//
LOCUS       DAOSEW010000006        13755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_3709_length_13755_cov_4.454745, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000006 DAOSEW010000000
VERSION     DAOSEW010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 13755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13755
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3709_length_13755_cov_4.454745"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..141)
                     /locus_tag="PLJ02_00465"
     tRNA            complement(67..141)
                     /locus_tag="PLJ02_00465"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(107..109),aa:Asn,seq:gtt)
     gene            complement(224..955)
                     /locus_tag="PLJ02_00470"
     CDS             complement(224..955)
                     /locus_tag="PLJ02_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04932.1"
                     /translation="MKLISLNIEGDKHLDDKILPFFDKEKPDVLCLQEVFAKDIQKIC
                     QRTGLEKYSFVSQAKVTKDNPHLPTNGLWGLCIFAKNLAKTRKHYYTGNANQIPEFFA
                     YQDPNSMNRCLLTVQTEIEGDIYQLGTTHFTWSGEGKINALQKENFVKLKAALSNYPE
                     IIFCGDLNTPRGEAIYDQLAKIYKDNIPQKIKTTIDKKIHKSKKDIQLVVDALFTSPA
                     YVAKKVKVVAGVSDHMAVVAEINKK"
     gene            complement(973..2148)
                     /gene="rpoD"
                     /locus_tag="PLJ02_00475"
     CDS             complement(973..2148)
                     /gene="rpoD"
                     /locus_tag="PLJ02_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOI04933.1"
                     /translation="MAKSTTPAKTPKKVELKQDQEPLVKTEKKKKPPKSLLAEVKNLI
                     KVGKKRGYLSQDEILDVFVEPEIYIELLDDFYDQLFQKNIDVFESVSEEQLEEDEDVL
                     QQELDVLANLGNVGVSDPVRMYLKEIGRIPLLTREEEIRLAQKVEAGSEKAKKDLIDA
                     NLRLVVSIAKKYIGRGMTFLDLIQEGNKGLIRAVEKYDWTKGFKFSTYATWWIRQAIT
                     RSIADQARTIRIPVHMVETINKLMRTSRRLMQELGREPTPEEIGEKMDLEPEKIRAIL
                     KISQNTTSLEMPVGDGEDDSTLGDFIADDKQVSPYDSTSKQMLTENVEEVLKALSDRE
                     AKVLRMRFGLAGNRTMTLEEVGREFGVTRERIRQIEAKALRKLKHPSRRKKLQDFLE"
     gene            complement(2301..3077)
                     /gene="map"
                     /locus_tag="PLJ02_00480"
     CDS             complement(2301..3077)
                     /gene="map"
                     /locus_tag="PLJ02_00480"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOI04934.1"
                     /translation="MIIHSTQELKRYQQAADKSVKILKKLFEATIEGATPQQIDNLAD
                     RECQKLGVIPCFKGVGPKNNKYRWATCISVNDTVVHGIPENEPLKKGDLVKVDFGIND
                     RGWITDHCFSKSIGRPQPAEQELLTVTRRAIIAGAKLAVVGKKTGDMGAKIQEIVESA
                     GFSVVKEFVGHGAGHTMHDSPQLPAYGQKGTGVALKEGMILCVEAQILGSRNDQVYVE
                     DNGWTVKTVDDAKAAMFEYMVMVGKKEAIFLTDTRDWPLF"
     gene            complement(3148..4365)
                     /locus_tag="PLJ02_00485"
     CDS             complement(3148..4365)
                     /locus_tag="PLJ02_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008104708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOI04935.1"
                     /translation="MKKEVSPYQPPAKILKKYAQVLVNYALNSGAGVHPGEIVQCAVP
                     DVAKPLALELQNEILRAGAHPLLNLIPTGFEKDFYNLANQEQLEFFPRRFWRARADLI
                     DHQINIIAEPDPFILKDIDPSKILRARNSKKAYRDWLNFKENANQFTWTVALWGTEAK
                     AKIVGLSLKDYWQQIIQACFLDEAEPIAKWRAIQTLQLAIKEKINNLKINYLQVVGPD
                     VNLKIGIGEDRIWKGGGGRNIPSFEIFTSPDWRTIDGWIHFNQPLYRYGNLIEEVELE
                     IKAGRVIKARAKTGERLLLEMLKTKNADKIGEFSLTDKRMSRISHVMAETLFDENIGG
                     PYGNMHLALGMAYQDCFRGDPKQLKKRDWAAKGFNDSSEHTDIVSTTDRTVTAFLTNG
                     QSQVIYRQGQFLL"
     gene            complement(4436..7612)
                     /locus_tag="PLJ02_00490"
     CDS             complement(4436..7612)
                     /locus_tag="PLJ02_00490"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOI04936.1"
                     /translation="MTNQPIHKSPAKKLSAIRSSTKKPFAKKPSAFQKAYQKLNPLQK
                     QAVDHIDQGPLLVIAGPGTGKTQVLALRIANILLKTDAKPDNILALTFTDVAAKNMRE
                     RLLKIIGKEAYYLQIHTFHSYCSQVMTNYPEYFPLKNNSEALEDLERYQIFENLIDQL
                     KPKHLRPLNDHYHYLKDIIKAISDLKREGISLLEFEEIIEAEKKRLVNLQQESKSKTK
                     IKQASKELAKNKELLLIYQAYQQELVESNRYDFDDMINFVIEAFEKYPELLLEQQEQL
                     QHFLVDEYQDSNNSQTRLLNLLASYWPQQGLEPDVFAVGDPNQAIYRFQGASVENVLA
                     FTRQYPTATVITLNTAYRCPQNLYDVASELIKNNQLSKFSGQEIKIDLTGKLYSPKGP
                     GEKIVLHVAPTATLEVIYIVEEIKKLLAKGVKAQEIVVLYRTHQEAKELIEVLNKWQI
                     AYQLNKGDDILQSELINQLLLVMGLIDQLQQGLELADLYQVMLLPFVGLDPMLVMKLA
                     RVAGQLSRIQRANVSIYQVYNMGLAKVNQQLEQQTITEEQWSTIQEFFAKLTSLAVYS
                     QQITLTAWFEKLIGNEGFKLLPYLKKQPDKIKSLLELNTLFKQIKNLSARDHQLRLNK
                     FLEYFATYREHQLSIRLQNPLPVENAVQLSTAHGAKGMEWDYVFVLDFVDGKWGNLRN
                     KNKVKLPEGILKHTDLSIKERNEDERRLFYVVLTRAKKRLYLSYPESVESNATVSNKV
                     PSIFLTELAEQELEKSEIDLSKDVEAYLTALLTAPVRTTISVQEKDFFTYLLKDFSLS
                     VTALNNYLRDPQSFVTNNLLKLPAAKEVSMCFGTAVHSALERFYRFYQQQNQYPSLDS
                     FLADFAQALDQEILSASDFQDRLTYGQEVLTKYYQFITQEKPQVLGVERSFGNKGHRI
                     VLGDNFLTGKIDRIDWLDQKQGLVKVIDYKTGRSKSLNHILVKTTTAAKDLSEREAEL
                     PATIRGSYQRQLVFYKLLMQLDRQLSPKIEVAEAAFNFVEPTEKGGDKFVSRSLVISD
                     EAIVDLKQLIKEVMAEIRSLKFLENIDF"
     gene            complement(7605..8672)
                     /locus_tag="PLJ02_00495"
     CDS             complement(7605..8672)
                     /locus_tag="PLJ02_00495"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HOI04937.1"
                     /translation="MEKKPLLALAPMDGVTDFAFRSIVKKHGQPDLIWTEFVSVEALC
                     RGAAPELMSQFDYDATQQPIIAQVFGQTPEYFYLVALMLAELGFAGVDINMGCPAKTV
                     AQSGSGAALIAKPDLAQAIIKQTQQAVEDWANGKSLSDCEKVNHKLLRALEEQKAFWQ
                     IQNVQRKLLPVSVKTRLGIKQNELQSWLPTLLATKPDLITIHGRTLKQGYSGQADWQA
                     LTTAGKMIKDFDENIQFFANGDVKNYEDALAKAELSGADGIMIGRASFGNPFVFLPEA
                     KRRQLTNSKNLFAVAIEHAKLFEEHNLARGKDKFFSMRKHLAWYIKAIPGAAQLRTQL
                     VKTNSASEVEQILANYQTKDD"
     gene            complement(8679..10412)
                     /locus_tag="PLJ02_00500"
     CDS             complement(8679..10412)
                     /locus_tag="PLJ02_00500"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HOI04938.1"
                     /translation="MGRVQAQVHQEFWQLADGSLAPELWESVLGDWDQWQVVEGRLHG
                     LLPAPPMFSGTRSAITPRFSLWPVTGNYQFSFDFTPLDTADKNFGVLFDYYIDYKGKV
                     ILSYLSFHFIEQQVYIETFQRNFLTHKTLVPWPLNPGETYHFRLIFQQPDFTLYVNDQ
                     LLFSSANDQDFWPIFLEPGRPLFYLTKGNYDQSAVIYSNFVLEYYPQLLVTYFSQLHA
                     SWAEVVYDHTQEFFSPALTIGNSGCALTSAVMLLHYYGFDTFPDQESWSSELRGKTIN
                     PETLNLWLKSEADGYLGVGLVNWLAITRLTKLLSQSVANKQFLAFAYADYQPDLVSEE
                     LGAGRPVIADLDGHFVVITGMAENGDHEEAISYLINDPVDSEHTMLDPKIEPIQSLRL
                     FKRNQTDLSYFLLLSAEELDFKLMHVADDLERPAVVAREANLLEANLPEANLPEVDLP
                     EANSPEANQDYFLYYWPQPENGEYLWQFEVNDLAKLPRAQLFVYQRDAQLQIFNLADY
                     VARELNFVFSKDQLAELAVDLNQHNLLFYHQYLLARLLKLQREQELLGEVANANRYQQ
                     LIEYFLDFYQL"
     gene            complement(10550..11950)
                     /locus_tag="PLJ02_00505"
     CDS             complement(10550..11950)
                     /locus_tag="PLJ02_00505"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOI04939.1"
                     /translation="MRKLYWYITLYIRRHGWVFLASIVATILFFSVLLPLLLRNLTFK
                     KQNYIAVVGQYNLSNLPTIIKQQLSAGLTSVGQDLSAQPMLAEKWIVEDDGKQYRFVL
                     KKGVYWQDGKELFPEDVYYNFVDVNIVTTPNDVIFNLPEPFAPFPLAVTEPILRSTTQ
                     KRFLLPNTTKIIGIGQYEMLDYKITGNYLEEVRIDGPEERYIYRFYPTEDAAVLAFKK
                     GEVDILLDLASCHDLCDWSNLEIVKDLDYNSYLALFFNNADPQLTKNVRQAFSYALEK
                     DYGEARANSPFNPHSWAYLEGGKDYQKDWTRGIERLIDELPRDPLNFELTTTTIFQDE
                     AERIQKELNSFGQEAIRVCRESADVEDKELCDNLGINLRIKISNFPDTNNFQLLLMGQ
                     SIPTDPDQYFMWHSTQATNFTRYKNTRIDSLLERGRQTIDTEERKSIYQEFQQFLLED
                     PPAVFLRYLERFSIAR"
     gene            complement(11954..12172)
                     /gene="secG"
                     /locus_tag="PLJ02_00510"
     CDS             complement(11954..12172)
                     /gene="secG"
                     /locus_tag="PLJ02_00510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOI04940.1"
                     /translation="MNNVFLITQAAISLVLIILVLLQSNASSSSSLFGGGGGETYHTR
                     KGIEKLFFYATIVLGVLFVVNSLLLLIY"
     gene            complement(12207..13700)
                     /gene="rny"
                     /locus_tag="PLJ02_00515"
     CDS             complement(12207..13700)
                     /gene="rny"
                     /locus_tag="PLJ02_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOI04941.1"
                     /translation="MSKVKVKESLSQAQPKVQQQPNEKIAADVESIIRDARSKAREII
                     IEAKDEVLEMRSKAEKESRRIEHDLAEQQRTLQNKLDKLDYRFDQIEQKEKRIDQLKE
                     DLVKQSEEIEKSKAQVLKKLEELSGYSKEEAKEYLLEGLKKKLSHDLAKLVAEKEEQA
                     KLEADDKAREILIDAMRHGATDYVAEYTVSVVNIPSEEIKGKIIGKNGRNIHAFERKT
                     GVDIDLDTSNTEIKLSSFDPVRREIARVAMERLIKDGRIQPTRIEEIVDKVTEEIDRI
                     TFDAGKKLCHEVGVYNIPNGLIAMLGKFKYRFSYGQNLIKHTIEETKIGSKIAQELGL
                     DVNTVKLGCLLHDIGKVSDDPDENHVQAGIRIAKKYNMPPAVIACIAEHHEDQPFSSP
                     ESVAVYIADAISGARPGARYENHEEYMKRMETLEAIATSYDEVKRAYAVQAGRELRVI
                     LIPEKSKDDDVILLANSIRDRVAKEVIVPGTVTVTVIRELRGQAQTK"
BASE COUNT         4137 a         2879 c         2306 g         4433 t
ORIGIN      
        1 ataccaagta aacaactgaa accattgatt aaaagtggaa aaatctcagt ccgagtagac
       61 gatgtttggc tcccccggta ggagtcgaac ctacaacctt gaagttaaca gcttcctgct
      121 ctgccattga gctacagggg atcttaaatg agccgttgcc ttattaaatt aaagcaacaa
      181 tagaatttta tcaaaaagaa acaaaaatac cagccaaaaa ccactatttc ttattaattt
      241 ccgccactac cgccatatga tcactgactc cagcaactac tttgactttt ttggctacat
      301 aagctgggct cgtaaacagc gcatcaacaa ctaattgaat gtcttttttt gatttatgaa
      361 tttttttatc aattgttgtt ttaatttttt ggggaatatt gtctttgtat attttagcta
      421 attggtcata aatggcttca cctcgcgggg tattgagatc gccacaaaaa ataatttctg
      481 gataattgct taaggcagct tttaatttga caaaattttc cttttgcaaa gcattaattt
      541 tcccctctcc tgaccaagta aaatgagtag tgcctaattg gtagatgtca ccttcaattt
      601 ctgtttgtac tgttagcaaa caacgattca tcgaattggg gtcttgatac gcgaaaaact
      661 caggaatttg attggcattg ccagtgtaat aatgtttacg tgtttttgct aaatttttgg
      721 caaaaataca caaaccccac aaaccattag ttggtagatg tggattatcc ttagtcactt
      781 tagcttgaga aacaaagcta tatttttcta aaccagttct ttgacaaatt ttttggatat
      841 ctttggcaaa aacttcttga aggcataaaa cgtctggttt ttctttgtca aaaaatggca
      901 ggattttatc gtctaaatgt ttgtcacctt caatatttaa agaaattaat ttcaccttaa
      961 cccatctaaa tgttattcca aaaaatcctg taattttttg cgtctactgg gatgttttaa
     1021 tttacgcaaa gccttagctt caatttgtcg aatacgctct cttgtcactc caaattcgcg
     1081 acccacttcc tctagggtca tggtgcgatt gccagctaaa ccaaaacgca tccgtagcac
     1141 cttagcttcc cgatcggata gggctttgag cacttcttcc acgttttcgg ttagcatctg
     1201 tttactggtt gaatcatagg gagaaacctg tttgtcatcg gcaataaaat caccaagggt
     1261 gctgtcatcc tcaccatcac caactggcat ctctagtgaa gtagtgtttt gagaaatctt
     1321 gagaatggcc cgtatttttt ctggctcaag atccattttt tcaccaatct cttctggagt
     1381 tggttctctg cccaattctt gcattagacg tctagaagta cgcattaatt tattaatggt
     1441 ttccaccatg tgtactggga tacgaatggt acgagcttga tcggcaatgg agcgagtaat
     1501 ggcttgacgg atccaccaag tagcataagt ggaaaattta aaacctttag tccagtcata
     1561 tttttctact gcgcggatca aacctttgtt gccctcttga attaaatcta ggaaagtcat
     1621 cccccgacca atatattttt tggcaataga cactaccaaa cgtaaattag cgtcgattaa
     1681 atctttttta gctttctcac tcccggcttc tactttttgc gctaagcgga tttcttcttc
     1741 acgggttaaa agaggaatgc gaccgatttc tttaagatac atccgcactg gatcggaaac
     1801 accgacattg cctaaattgg ctaaaacgtc aagttcttgt tgtaatacat cctcatcttc
     1861 ctctaattgc tcttcagaaa cagactcaaa aacatcaatg tttttttgaa aaagttgatc
     1921 ataaaaatca tccaagagtt caatatatat ttctggttcc acaaaaacat cgagaatctc
     1981 atcttgagat aaatagccac gcttcttacc aactttaatc aaatttttga cttcagctaa
     2041 taaagatttt ggtggtttct tctttttttc agttttgacc aatggttctt gatcttgttt
     2101 taactcaact ttttttggag ttttggctgg ggttgtactt tttgccataa atttgtctat
     2161 tttgatttat taaaataaaa aaatcactgt caagtctttt tctatggcga ctgcctattc
     2221 accagagaaa agttcagtgt agcgagtgta taattataca aaaataattg aacaaaagca
     2281 agcaccaaag ctaaaaaagc ttaaaacagt ggccaatcgc gagtatcggt gagaaaaatt
     2341 gcttcttttt ttccaaccat taccatatat tcgaacatgg cggccttagc atcatctacc
     2401 gttttcactg tccaaccatt atcttcgaca taaacttgat cgttgcgact acccaaaatc
     2461 tgcgcttcga cacacagaat catgccttct ttgagcgcaa caccagtccc tttttgcccg
     2521 taggctggta attgagggct atcatgcata gtgtgacctg ctccatgacc aacaaattct
     2581 ttcaccacac taaaaccagc actttcaact atttcctgta ttttggcacc catgtcccca
     2641 gtttttttac cgaccaccgc taacttagcc ccagcaataa tcgctcttct agtgacggtt
     2701 aataactctt gctcagctgg ttgtggccga ccaatcgatt tagaaaaaca gtgatcagta
     2761 atccaacccc gatcattaat cccaaaatct actttcacta aatcaccttt tttcaagggt
     2821 tcattttctg gaataccatg caccacagtg tcattgacgg aaatacacgt tgcccaacga
     2881 tatttgttat tttttggacc aaccccttta aaacaaggaa taacccccag tttttgacat
     2941 tcccgatcag ctaaattatc aatttgttgg ggagtcgccc cttcaatagt agcctcaaat
     3001 aattttttga gaattttcac cgacttatca gcagcttgct gatagcgttt taattcttgt
     3061 gtagagtgaa taatcataat cttattgtaa caccaagttg agtagggtta acttttatag
     3121 ccaaaaagcc aattcaagaa tagctaatta aagaagaaat tgaccttggc gataaatcac
     3181 ctgactctgg ccattagtta aaaaagccgt caccgtgcga tcagtagtcg aaacaatatc
     3241 agtatgttca ctactatcat taaaaccttt agctgcccaa tcacgttttt ttaattgctt
     3301 aggatcacca cgaaaacaat cttgataagc catacccagc gccaaatgca tattgccata
     3361 aggaccaccg atattttcat caaaaagagt ctctgccatc acgtgactaa tgcgtgacat
     3421 acgcttgtct gttaaagaaa actcgccaat tttgtcagca tttttagtct tgagcatttc
     3481 caataataaa cgctcgccgg ttttagctct ggccttaatt acccgaccag ctttaatttc
     3541 tagctccacc tcttcaatta aattcccata acgatataag ggctgattaa aatggatcca
     3601 accatcaatt gtccgccagt ctggtgaggt aaaaatctca aaactgggga tattgcgacc
     3661 accaccacct ttccagatgc gatcttcacc aatgccaatt tttaaattaa catctggacc
     3721 aactacttgc agataattaa tttttaaatt gttgatcttt tctttgatgg ctaattgtag
     3781 agtctgaatt gctcgccatt tggcaattgg ctctgcctca tccaaaaaac aagcttgaat
     3841 tatttgctgc caataatctt ttaagcttaa accaactatt ttggctttag cttctgtccc
     3901 ccacagagct accgtccaag taaattgatt agcattttct ttaaaattta accaatcacg
     3961 ataagctttt ttgctattac gcgctcgtaa aattttactc ggatcaatgt ctttcaaaat
     4021 aaatggatct ggttcagcga taatattgat ttggtgatca atcaaatcag cccgagctcg
     4081 ccagaagcgt cttggaaaaa actctaattg ttcttgatta gctaaattat aaaaatcttt
     4141 ttcaaaacca gtcggtatta aattaagcag aggatgagca ccagcacgca aaatttcgtt
     4201 ttggagttcc aaagccaaag gtttagccac gtcaggtacc gcacattgca caatctcccc
     4261 aggatgaaca cctgcaccag aattcagcgc atagttaact aaaacttgtg catacttttt
     4321 taaaatcttg gctggtggtt gataaggaga tacttctttt ttcatttttt catcaatctt
     4381 gctctttaca agctttgcta ttataaacaa atttctcttg ttgaaaaccc gaaagttaga
     4441 agtcgatatt ttctaaaaac ttgagcgagc gaatttcagc catgacttct ttgattaatt
     4501 gtttcaaatc tacaatagcc tcatcactga tcaccaaact gcgactgaca aatttatctc
     4561 cacctttttc tgttggctcg acaaaattaa aggctgcttc agctacctca attttaggac
     4621 tgagttggcg atcaagttgc attaacaatt tataaaaaac taattgccgt tgataactac
     4681 cgcgaatcgt tgctggcaat tctgcttctc gctcagataa atctttggca gcggtggttg
     4741 ttttgactaa aatatgatta agactcttac tgcgaccagt tttgtaatca atcactttaa
     4801 ctaaaccctg tttttgatct aaccagtcaa tgcggtcaat tttgccggtt aaaaaattgt
     4861 cacctaaaac aatgcgatga cccttattac caaaactgcg ctccaccccc aacacttgtg
     4921 gtttttcttg agtaatgaat tgataatatt tagtcaatac ttcttgacca taagttaaac
     4981 gatcttgaaa atccgatgca ctcagtattt cctgatcaag cgcttgggca aaatcagcca
     5041 aaaaactatc caaacttgga tattgatttt gttgttgata aaaacgatag aacctttcca
     5101 aagcactatg gactgcagtg ccaaaacaca tagacacttc tttggctgcc ggcaacttta
     5161 aaagattatt agtcacaaaa ctttgcggat cccgcaaata gttattaagg gctgtcaccg
     5221 atagagaaaa atctttcagc aaatatgtga aaaaatcttt ttcctgaaca ctaatggtcg
     5281 ttcttactgg cgctgttagt aaagcggtta aataagcttc cacatcttta ctgagatcaa
     5341 tttcactttt ttctaactct tgctcagcta attcagtcag aaaaatactc ggcaccttat
     5401 tgctcaccgt ggcattactt tctactgatt ctggataact caaatacagc cgttttttgg
     5461 ccctagttaa gaccacataa aacaggcgtc tttcatcttc gttacgttct ttaattgata
     5521 aatcagtgtg ttttaagata ccttctggca attttacttt atttttattg cgcaaatttc
     5581 cccatttgcc atccacaaaa tccaacacaa aaacatagtc ccactccata cctttagctc
     5641 catgagcggt ggatagttgt accgcgtttt caactggcaa aggattttgt aaacgaatgc
     5701 ttaattgatg ctctctataa gtagcaaaat actctaaaaa tttatttaat cttaattgat
     5761 ggtcgcgggc gcttaaattt ttaatttgtt taaataaagt atttagttcc aaaaggcttt
     5821 tgattttgtc gggctgtttt tttaaatatg gcaatagttt aaaaccttca ttgccaatta
     5881 atttttcaaa ccaggctgtt agggtaattt gttggctata tacagctaag ctagttaatt
     5941 tcgcaaagaa ttcttgaata gttgaccatt gttcttcagt gattgtttgc tgctcaagtt
     6001 gctgattaac tttagccaaa cccatattat aaacttggta aatagaaaca ttagcccgct
     6061 gaatccgact taattgacca gcgactctgg ccaatttcat aaccaacatt ggatctaaac
     6121 ccacaaaggg caataacatc acctgataca aatcagctaa ctccaaaccc tgttgtagtt
     6181 ggtcaattaa ccccattacc aaaagcaatt gattgatcaa ctcactttgt aaaatatcgt
     6241 cacccttatt taattgataa gcaatttgcc atttgtttag cacctcgatt aactctttag
     6301 cctcctggtg agttctatac aagaccacaa tttcttgagc cttgactcct ttggctaata
     6361 attttttgat ttcttcgacg atataaatta cttctaaagt ggctgttgga gcaacgtgta
     6421 gaacaatttt ttctcctgga ccttttggtg aatacaactt accagttaaa tcaattttga
     6481 tttcttgacc agaaaacttg gataattgat tatttttgat taactcacta gctacgtcat
     6541 aaagattttg cggacatcgg taagcagtat taagggtaat gactgtggct gttgggtact
     6601 ggcgagtaaa agctaaaacg ttttctaccg aagctccctg aaaacgataa atggcctggt
     6661 taggatcgcc aaccgcaaat acgtctggct ctaaaccttg ctgtggccaa taggaagcta
     6721 ataaatttaa caggcgcgtc tgactattat tactatcctg atattcatct accaaaaaat
     6781 gttgaagttg ttcttgttgt tctaataata attctggata tttttcaaat gcctcaatga
     6841 caaaattaat catatcgtca aaatcgtaac gattactctc gactaattct tgctgatagg
     6901 cttgataaat taataatagc tctttgtttt tagctaattc tttgctggct tgcttaattt
     6961 tagttttgct tttgctttcc tgttgcaaat tgactaagcg ttttttttcg gcctcaatta
     7021 tttcttcaaa ttctaagagg gaaatgcctt ctctttttaa gtcagaaatt gctttaataa
     7081 tgtctttcag gtaatgataa tggtcattta atggtctcag gtgtttgggt ttgagctgat
     7141 caattaaatt ttcaaaaatt tggtagcgct ccaagtcttc taaagcttca ctattgtttt
     7201 tgagtggaaa atattcggga taattagtca tcacttggct acagtaggaa tgaaaagtat
     7261 gaatttgtaa atagtaggct tctttgccga tgatttttaa tagacgctcc ctcatgtttt
     7321 tagctgctac atcagtaaaa gttaaagcta aaatgttgtc aggtttagca tcggtcttta
     7381 ataaaatatt ggcgatccgc agagccaaaa cttgagtttt accagttcct ggaccagcta
     7441 tgaccaacag aggtccctga tcgatatgat caacggcctg tttttgtaat gggtttaatt
     7501 tctgataagc tttttgaaaa gctgacggtt tttttgcaaa cggttttttt gttgacgacc
     7561 ttattgctga tagtttttta gctggtgatt tgtggatagg ttggttagtc atctttagtt
     7621 tgataatttg ctaaaatttg ttctacctcg ctggcgctat tagtttttac taattgagtg
     7681 cgtaattgag cagcaccggg tatggcttta atataccacg ctaaatgctt acgcatacta
     7741 aaaaatttgt ctttccctct cgctaaatta tgctcttcaa aaagtttggc atgttcaata
     7801 gccactgcaa ataaattttt gctatttgtt aactgtcggc gttttgcctc tggtaaaaaa
     7861 acaaaaggat tgccaaaact cgcccgtcca atcataatgc cgtccgcccc gctcagctcg
     7921 gctttcgcta aagcatcctc atagttttta acatcaccat tagcaaaaaa ttgaatgttt
     7981 tcatcaaaat ctttaatcat tttacccgct gttgttagtg cttgccaatc ggcttgacca
     8041 ctatatcctt gttttaaggt gcgaccatga atcgtaatta aatctggctt agtcgccaat
     8101 aatgttggta accagctttg taattcattt tgcttaattc ccagtcgagt ttttactgaa
     8161 actggtaata gtttgcgctg aacgttttgg atctgccaaa atgccttttg ctcctccagt
     8221 gcccgcaata atttatgatt tactttttcg cagtcactta aactcttacc attggcccaa
     8281 tcctcaaccg cttgctgtgt ttgcttaatg attgcttgag ctaaatctgg tttagcaatt
     8341 agcgctgcac cagaaccact ttgggccaca gttttggctg gacaacccat atttatatcc
     8401 acaccagcaa aacccaattc ggccaacatt aaggctacta aatagaaata ttcaggagtt
     8461 tgaccaaaaa cttgggcaat aattggttgt tgagtagcat cgtagtcgaa ctgactcatt
     8521 aattcaggag cagctccacg gcaaagtgct tcaacactca caaattcagt ccaaattaaa
     8581 tctggttgac catgtttttt gacgatcgaa cgaaaagcaa aatcagttac tccatccatg
     8641 ggcgccaaag ctaaaagcgg tttcttttcc acaacttatt ataactggta aaaatctaaa
     8701 aaatactcaa ttagttgttg ataacgattg gcatttgcta cttcacccaa taattcttgc
     8761 tctcgttgca gttttaataa gcgagctaat agatattgat gataaaaaag caaattatgt
     8821 tgattaagat cgacggcaag ctcagctaat tgatctttac taaaaacgaa gttaagctct
     8881 ctagctacat aatcggctaa attaaaaatt tgtaactgag cgtctctttg gtaaacaaac
     8941 aactgagctc gtggtaattt tgctaaatca ttaacctcaa attgccacaa atattcgccg
     9001 ttttctggct gtggccaata atataaaaaa taatcctgat tggcctcagg tgaattggcc
     9061 tcaggtaaat caacctcagg taaattagcc tcaggcaaat tggcttctag taaattagcc
     9121 tcccgtgcaa caaccgctgg ccgttctaga tcgtctgcaa catgcattag cttaaaatct
     9181 aattcttcag cactcaataa taaaaaataa ctcaaatctg tttgattacg cttaaacagg
     9241 cgcagagatt ggattggctc tattttagga tctaacatgg tgtgctcgct atcgactggg
     9301 tcattaatta aatagctgat agcttcttca tggtcaccat tttcggccat gccagttatt
     9361 accacaaaat gtccatcaag atcagcaatc acgggccgac ctgcacccaa ctcttcacta
     9421 actaaatctg gctgataatc ggcgtaagca aatgccaaaa actgcttatt tgcaactgat
     9481 tgcgacaaca acttggttaa ccgagttata gctaaccaat tgacgagccc cactcctaaa
     9541 taaccgtcag cttcactttt aagccagaga tttaaggtct ctggattgat agttttaccc
     9601 cgaagctcac tcgaccaaga ttcttgatca ggaaaagtgt caaaaccata ataatgaagt
     9661 agcatcaccg cactggttaa agcacaacca ctattaccga tcgttaatgc tggactaaaa
     9721 aactcctggg tgtgatcgta gaccacctcc gcccaactag catgcaattg agaaaaatac
     9781 gtaactagta attggggata atactctaaa acaaaattag aataaattac tgccgattga
     9841 tcatagttac ctttagttaa ataaaataaa ggacgacctg gttctaaaaa aattggccaa
     9901 aaatcttgat cgttggcact ggaaaataac aattgatcat taacatataa tgtaaaatct
     9961 ggttgttgaa aaattaaacg gaaatgatac gtttctccag gatttagggg ccaaggcacc
    10021 aaggttttat gagttagaaa gttccgttga aaagtttcaa tatagacttg ttgttcaata
    10081 aaatgaaaac ttaaataact taggatgact ttacctttat agtcgatgta gtaatcaaac
    10141 aacacgccaa aatttttatc ggcagtatcc agcggagtaa aatcaaagga aaattggtaa
    10201 ttgccagtaa caggccagag tgaaaagcga ggcgtaatag cactcctggt accagaaaac
    10261 attgggggcg ctggcaacaa accgtgcaag cgcccctcaa ctacctgcca ctggtcccaa
    10321 tcacccaaca ccgactccca cagttcaggt gccagtgatc catccgccaa ctgccaaaat
    10381 tcttgatgaa cttgcgcctg cactcgcccc attgaactat taaaaatcaa gaacaataaa
    10441 ataagctggc ttagaaaaaa tttagatttt tgagtaatgg caacaaattt ggtcactcat
    10501 taatcatgac caaaataatt gacaactaaa ctgacaaaaa actaagcaac tagcgggcaa
    10561 tagaaaagcg ctccaaatag cgcaaaaaaa ctgctggggg atcttctaat aaaaattgtt
    10621 gaaactcttg atagatactt tttctttcct cagtgtctat agtctgtcgg cccctctcta
    10681 acaaactatc aattctggta tttttatatc gagtaaaatt agtagcttgc gtactgtgcc
    10741 acataaaata ttggtcagga tcagtgggga tactttgacc catcagtaat aattgaaaat
    10801 tattagtgtc gggaaagtta ctaatcttaa ttcgtaaatt aataccaaga ttgtcacaca
    10861 attctttatc ttcaacatca gcactttcac gacataccct gatggcttct tgaccaaaac
    10921 tatttaactc tttttgaatg cgttctgcct catcttgaaa aatggtggtc gtcgtcaact
    10981 caaaatttaa gggatctcta ggtaattcat caattaaccg ttctatacct cgcgtccaat
    11041 ccttttggta atccttacca ccctccaaat aggcccaaga atggggatta aatggactat
    11101 tagctcttgc ttcaccataa tctttttcta aagcataaga gaaggcctga cgcacatttt
    11161 tggttaattg tggatcagca ttgttaaaaa atagagctaa ataactgttg taatctaaat
    11221 ctttgactat ttctaaattc gaccaatcac aaagatcgtg gcaactggcc aaatctaata
    11281 gaatgtcaac ttcacctttt ttaaaagcta aaactgcagc atcttcggtg ggataaaagc
    11341 gataaatata gcgttcttct ggaccatcaa ttcgcacttc ctctaaataa ttaccagtga
    11401 ttttataatc taacatttcg tattgaccaa taccaattat tttagtggtg ttgggtaata
    11461 agaaacgttt ttgcgttgtg gagcgcagga ttggctcggt cactgccaaa ggaaacggag
    11521 caaacggctc aggtaaatta aaaattacgt cattgggggt cgtcacaata ttaacatcaa
    11581 caaaattata ataaacatct tctgggaata actctttgcc gtcttgccaa tataccccct
    11641 tttttaaaac gaaacgatat tgtttaccat cgtcctcaac aatccatttc tctgccaaca
    11701 tgggttgggc acttaaatcc tgaccaacac tagttaaacc agcactgagc tgctgtttga
    11761 taattgtcgg caaattcgac aaattatatt gaccaactac agcaatgtaa ttttgttttt
    11821 tgaaggttag atttctcaat aataatggca acagtactga gaaaaacaga atggtagcaa
    11881 caatcgaagc cagaaacacc cagccatgac gacggatgta taaagtaata taccaataga
    11941 gtttgcgcat aatttagtag atcaataaca aaaggctatt cacaacaaaa agcactccca
    12001 aaacaatagt tgcataaaaa aacaattttt caatcccttt gcgagtgtga taagtttctc
    12061 caccacctcc accaaataaa ctgctcgaac tgctggcgtt gctttgcaat aacaccaaaa
    12121 ttatcaacac caaggaaatg gctgcttgag taatcaaaaa aacgttattc ataattttgc
    12181 attctacttt aatttctcat tttactttat ttggtttgtg cttgtccacg taactcacga
    12241 atcaccgtca ctgttacagt tcctggcaca attacctctt tggctactcg gtcacggata
    12301 ctattagcca acaaaattac atcgtcatct ttgctttttt ctgggattaa aattactcgc
    12361 aactctctac cagcttgtac agcataagcc cgtttgactt catcataaga agtggcaatt
    12421 gcttccaagg tttccatccg cttcatatat tcttcatgat tttcataacg agcgcctggt
    12481 cgagccccag aaatagcgtc agctatataa acagccactg actctggact agaaaagggt
    12541 tgatcctcat gatgttcagc aatacaggca ataactgctg gcggcatgtt atatttctta
    12601 gcaattctaa taccggcttg cacatgattt tcatcgggat catctgaaac ttttccaata
    12661 tcatgtaaca aacaacccag ttttacagtg ttaacatcca aacccagctc ttgagcaatt
    12721 ttgctaccaa ttttggtttc ttctatagtg tgtttaatca aattctgtcc ataggaaaag
    12781 cgatacttaa acttgcccaa catagcaatc aaaccattag gaatgttata aacaccaact
    12841 tcatgacaaa gttttttgcc agcatcaaaa gtaatgcggt caatttcttc agttacttta
    12901 tcgacaattt cttcaattct agttggctga atccggccat ctttaatcaa tcgctccata
    12961 gccactcggg caatttcccg acgcactgga tcgaaagaag ataatttaat ttcagtgtta
    13021 gaagtatcta aatcgatatc gacaccagtt ttacgttcaa aggcgtgaat atttcgacca
    13081 tttttgccga taattttccc cttgatctcc tctgagggaa tattgactac tgacacggtg
    13141 tattctgcaa cataatcagt cgccccatga cgcatggcgt caattaaaat ttcccgagcc
    13201 ttatcgtcag cctccaactt agcttgttcc tctttttcag ccactaattt cgctaaatca
    13261 tgacttaatt ttttctttag tccctctagt aaatattctt tggcttcctc tttggagtaa
    13321 cccgataatt cttctaattt cttgagtact tgcgctttag atttttcaat ttcttcactt
    13381 tgtttaacta aatcttcctt aagctgatca attctttttt ccttctgctc gatttgatca
    13441 aaacgataat ctagtttgtc taacttattt tgcaaagtac gttgctgctc agctaaatca
    13501 tgttcaatgc gacgagattc tttttctgct ttggagcgca tttctaagac ttcgtcttta
    13561 gcctcaataa taatttctct agcctttgag cgagcatcac gaatgatgct ttcgacatca
    13621 gccgcaattt tttcattagg ttgctgttgg actttaggct gagcttgact tagactttct
    13681 ttaactttaa ccttgctcac ttggcttggt tttttaactt gactttgttc ctgtttttgc
    13741 tcttttactt gagct
//
LOCUS       DAOSEW010000007        13272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_3906_length_13272_cov_4.266929, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000007 DAOSEW010000000
VERSION     DAOSEW010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 13272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13272
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3906_length_13272_cov_4.266929"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="PLJ02_00520"
     CDS             <1..276
                     /locus_tag="PLJ02_00520"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOI04942.1"
                     /translation="QAKLLTQQERNKISVAYEPVSAIGSGQAVAPPQVSKFFTKLRQY
                     FPFNPLIYGGSVDPSNIKDFLAISDGVLVSSAALEINDFVRLMQAII"
     gene            369..1736
                     /locus_tag="PLJ02_00525"
     CDS             369..1736
                     /locus_tag="PLJ02_00525"
                     /inference="COORDINATES: protein motif:HMM:NF027518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HOI04943.1"
                     /translation="MKIKEIIFLGLILVVSLLVRLWRLDIPQEMYFDEIYHVPAVQLL
                     AAGDFQTPFEWWHQADWPIYYDWLHPPLAKYIQALSLNFFDFLPAPVAWRLPSVIFSL
                     MSLLVFYFFAKDVALSLLTRQKIDQRLFNKQKVEDLAINFALISSLLLASDGLFLVQS
                     RIAMNDIFYLFFALAASYCYFQYYRHQNLIVLFLTGVMLAFALSTKWSAIWLIGFFFI
                     LELYQLVKEKKLQQLPFTIFSLLLTPVFVYFLIFTPYFLSGKTLTDFYQLQKQIIIFQ
                     QNWASPHPYQSSPLAWMINWRPVWYFADLTENNWRKDIFAQGNPVLFLYFLFLPIFLW
                     WWQRSLKTKRAITKWRNLLVQKTYLLLICLFFASFLPWIVVTRPMFFYHFLPALPYLI
                     LIVTWPIYLFILSRQSQQQRQLLLFHFLFWPVLIAIIFYPHWTALPVPEAASNYLYFV
                     LPSWR"
     gene            1774..3120
                     /locus_tag="PLJ02_00530"
     CDS             1774..3120
                     /locus_tag="PLJ02_00530"
                     /inference="COORDINATES: protein motif:HMM:NF005560.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOI04944.1"
                     /translation="MTRIAIIGGGFGALNLAWRLANLDSKEQLGIDIFEKEAQLGGMA
                     GGFQEKNWAWTFEKHYHHLFAKDRAFQNFLKSLDLSDKLFYRKTLTSTLWQQQIYQLD
                     TAWSLLNFTELSFIDRLRTGLVLAFLKIIPNGQFLEKYRASDFLQKTMGQKSWQIIWQ
                     PLFQAKFADWADDINLAWFWARINPRTAKLGYVEGGFQSLANLVQQKLAKRGVKINLQ
                     CIVKKISPVKSTDQQNSRFKLKFISTKDTANKQPSSTSQTQEFDLIINTLPSPQFSQL
                     IDLPELKSAHLQGLAAMTMILRLKDQLLLDNTYWLNVNERDWPFLAVVEHDHFVDQRF
                     YNHEHLVYIGKYLSTSDPVYQFSAKKLWQSYLPFLRQLNPKIEEILIDYRVTKEAFAQ
                     PLVKTNHSRNLPSLRTSYPNLYWVSMQHIYPFDRGLNQAIVSSNRLFELIKKDLKI"
     gene            3173..5359
                     /locus_tag="PLJ02_00535"
     CDS             3173..5359
                     /locus_tag="PLJ02_00535"
                     /inference="COORDINATES: protein motif:HMM:NF021570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HOI04945.1"
                     /translation="MILAVYLSLLFLQLLAVPFLAWVSKLFPQIKDGLWAFGRYLTWL
                     VLSLIIFFLAHLKLAVNNVSGVYFLIFVLVLVNCYFLFRAKNQQKLLSSIKHYWQHFH
                     RQIIIEEVLFILFFAFLLVVRSFQPEILGLEKFMDAGLMQSYLKSPTLPAGDMWLAGE
                     KINYYSFGHFMGSILVQIWGIDMAWAYNFLLATIMAILASSVFSLTFNIRANFTWGQK
                     IGRDFSAKEKYNFSATVGTALLATVMVTLFGNGHSLWFFLSNGNLTNYFYPSATRFIA
                     NTIHEFPVYSFVVSDLHAHVFSLPMVVAMLMSIFLWLSELIEEVKRQKVLKLFTERFF
                     ILSLVMGVFIGALAMTNTWDVLIYSVFLLILGILLLMWKKEFWLPLVSSALGLSLVAA
                     MVSAPWFLTFQSIAEGIKIASEHSPLWQLLVLWGAHLIPVLIFILLLLFHSQRREPKE
                     ETSWFFVVAMFFTFVFLLIFTELFYFKDIYTSYPRANTMFKLMFQGFILIAILLALAI
                     SFFISHAKTGKHFNWQLIDLREVWSQKNNQLLKIKTILRTGLFVYLPITLLIVGLATY
                     PYMAFRSYYGNFQHYQGLNGLKWMETRYPSSYAIVNYLKANEAQQVNILEASGESFTE
                     FSLISAFSGMPTVVGWQVHEWLWRGTWDIPAQRLREIDVIYNNPLSAESTASIEQYQL
                     KYIIITNRERAKFPNLNEKALLQLGRVVWTGSEDGINQDYLILLEK"
     gene            complement(5340..6134)
                     /locus_tag="PLJ02_00540"
     CDS             complement(5340..6134)
                     /locus_tag="PLJ02_00540"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOI04946.1"
                     /translation="MNNNNNSFFKFLVVFIVGFVSFYLLQNFTPLANSSFFNPEKSTI
                     TVQGSAQENILNQLAEFTAGIEVIAPEKEEAVNGATEILNQLLVEIKDFGISEKDIQT
                     TQVSVYQEDENTIQPLSNAETVLIEGNETGKTGDWRANISVTITLRNENSELLKSQSE
                     DLLSILNNSQANYVYGPNFRLDQENLSEIDLINSAVADARTKAEAIAANNQQQIVKVL
                     EIVESGANYQPFYRAESASSLDSSSPSLEPGSSAIAKTVTVTFQVK"
     gene            complement(6210..7289)
                     /locus_tag="PLJ02_00545"
     CDS             complement(6210..7289)
                     /locus_tag="PLJ02_00545"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOI04947.1"
                     /translation="MKIQSIIKRLWPLFLSIVIVSAAVFLHFNPPDTIALAGLQIEYL
                     GPDRYASVFLDDQYLEKAPLANRDIRSGDYILKIVPDDQTLAEFSTPITLTTGTLTVV
                     SYKPGPTTKESAATIYEIEKVADLKDFGQVSFESYPEHALLSFNGETTQYTPIVLENL
                     VPQEYQYRVSLPSYETQEHTLQVLAGYTIKVSIKLAKLAENPLDQAQVETSPEENKIA
                     SAAAQIDAAAQIDNGQDESWGVTDPKAITGAKVRIKQTAFFVDNQEVLRVRAAPNPTA
                     SELGLAKSGFYYPAAVVLPEDDENNDKVVKDEDVAEGGDVAEGGDVAEGGDSSSSAQR
                     ADWLKIKFEGQEGWVSARFAELIVD"
     gene            complement(7339..9684)
                     /gene="lon"
                     /locus_tag="PLJ02_00550"
     CDS             complement(7339..9684)
                     /gene="lon"
                     /locus_tag="PLJ02_00550"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOI04948.1"
                     /translation="MNSKIIQLPSNTFNLPVVPIREGVLFPQTESVLGFGREPSIKAI
                     RQAQKQDNHIILLTQRNPRVEQPRAKDLYSVGTLAVIEKTLQNADNVSTLVRGISRIK
                     IQKFVQEEPFLQAIGEKMVSFAERDDELIALSNQLQKLFRQTVQMGKQIEFLNFIKLL
                     SGVNEGEMADQIASTLNIATEKKQLILETIDVKQRIRLVIEYLSKEVKILEIEQDVNL
                     KTQEKFDEHMRESVLRERLRTIQKELGELDDDQDIINSYQEQLKKKKLPKTAEKKVKK
                     EIDRLEQMSPNNPEASYLRSWLDLVFDLPWQKSKKTNVNIKRAAKILNADHYGLEEVK
                     DRVLEYMAVMQLKNQNANQEKNLPTILCFVGPPGVGKTSIGRSIAKSLNRQFIKISLG
                     GVRDESEIRGHRRTYVGAMPGRIIKGLQNAQSNNPVFMLDEIDKLGRDFNGDPSSALL
                     EVLDPEQNFSFEDHYLDMPYDLSEVLFIATANTLDTVPPALLDRLEVIYYSGYTLNEK
                     FEIAKRYLLPKTIKNNALKKTQVKLSDAILKKIISRYTREAGVRDLERQLNKIMRKIA
                     REILEKNKKSITINQKKLTEFLGPAIYDETIAEKEDQIGLATGLAWTSVGGDVLFVEV
                     ALSPGKGRIILTGKLGDVMKESAQAALTYVRSHHQELKIDIEKINQTDVHIHVPEGAV
                     PKDGPSAGVTITTAIVSAFKQQPVRRDVAMTGEVTLRGRVLRIGGLKEKAIAAHRAGS
                     KIVIIPQENERDLVKIPKEVKKDLEFKTVADVKEVLKIALN"
     gene            complement(9778..9851)
                     /locus_tag="PLJ02_00555"
     tRNA            complement(9778..9851)
                     /locus_tag="PLJ02_00555"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9815..9817),aa:Ile,seq:gat)
     gene            complement(9894..12458)
                     /gene="clpB"
                     /locus_tag="PLJ02_00560"
     CDS             complement(9894..12458)
                     /gene="clpB"
                     /locus_tag="PLJ02_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOI04949.1"
                     /translation="MNFNQYTTKAAEAVQDAMQLAGKLKHSQIEVWHLLLALVEQPGG
                     IVPNLLEQLDTKPEAIASLIRKELQKLPVTETPTQAYASAELTKVLSQAETEAVKLKD
                     EYISTEHLFLALLEQKKVVQLLDLDPQKVVQALKTVRGQQRVTDPEPEGKYQALEKYC
                     LDFTSLARSGKIDPVIGRDEEIRRITQILSRRTKNNPVLVGEPGTGKTAIVEGLAKKI
                     IDDDVPEALKNKRVLALDMGALIAGAKYRGEFEDRLKAVIKEIEKSDGQIILFIDELH
                     SIVGAGASEGSTDAGNLLKPALARGTLRTIGATTLKEYRQYIEKDSALERRFQPVMVE
                     EPSVEDAISILRGIKSKYEAHHGVKILDNAIVAAVKLSHRYITDRFLPDKAIDLMDEA
                     ASALRIEIDSKPSSVDQLERKIRQLEIEKESLKKEKDQKSQDRLAKINKQISELKEKY
                     KSLELRWRKEKEILNSIKQYNQELDQLREEAKRAERNYDLERAATINYGEIPELEKKI
                     KEAQQKMASIDPEERLLKEEIDESDIASIVSKWTGIPVNRMLTEESERLIHLEEALAK
                     RVVGQEQAIKAVAGAIRRNRAGISEEGRPVGSFLFLGPTGVGKTELAKALAEQLFNDE
                     HLITRIDMSEYMEKHSVARLIGSPPGYIGYEEGGQLTEALRRHPYTIVLLDEIEKAHS
                     DVFNILLQVLDEGRLTDSKGRTVNFKNSIIIMTSNLGSQIISETSNDPVEQKKALDGL
                     LKMHFRPEFLNRLDDIIIFQSLGKKEIKAIVQLQLNLVAQRLAEREIQLEFSERLIEH
                     LAEAGYDHLYGARPLKRLIQNEILDELAEQIITGKIKTGDQLRLDWQKNKLDLVKT"
     gene            complement(12919..>13272)
                     /locus_tag="PLJ02_00565"
     rRNA            complement(12919..>13272)
                     /locus_tag="PLJ02_00565"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         3540 a         2589 c         2527 g         4616 t
ORIGIN      
        1 caagccaaat tgctgactca gcaggaaagg aacaaaatca gcgtggctta tgagccggtt
       61 tcagccattg gtagtggcca agcagtggcg ccaccgcaag tgagtaaatt ttttactaaa
      121 ctccgccaat attttccctt taatccacta atttatggtg gcagtgtcga tccaagcaat
      181 attaaagatt ttttagctat tagtgatggg gtgttggttt ccagtgctgc gttggagatt
      241 aatgatttcg tgcgattgat gcaagcgatt atttaatttg tcagtgatta tttaattact
      301 aattgcgcct gaattttttt aactctggac ttgaaaaaat gtactatttg tatatattct
      361 gatagatagt gaaaattaaa gaaattattt ttttaggact gattttagtg gtttccttat
      421 tggtgcgtct gtggcgacta gatattcctc aggaaatgta ttttgatgaa atttatcatg
      481 tgccagcagt gcaattgctg gcggccggtg attttcaaac gccttttgaa tggtggcacc
      541 aggccgactg gccaatttat tatgattggc tccatccccc gctagctaaa tatattcagg
      601 ccttgtctct caattttttt gattttttac cagcaccagt ggcttggcgt ttgccttcag
      661 taattttttc tttaatgtct ctcttggttt tctatttttt tgccaaagat gtggctttaa
      721 gcttactcac taggcaaaag attgatcagc gtctttttaa caagcaaaaa gtggaagatt
      781 tagccattaa ttttgctctg atctcgagtt tattattagc cagtgatggt ttatttttgg
      841 tgcaaagtcg gatcgcgatg aatgatattt tttatttgtt ttttgcctta gctgctagtt
      901 attgttattt ccagtattat cgtcaccaaa atttgatagt gttatttctc actggtgtaa
      961 tgcttgcgtt tgctttgagc acgaagtggt cggcaatttg gttaattggc ttttttttca
     1021 ttttggaact atatcagcta gttaaagaaa aaaaattaca acaactgcct tttactattt
     1081 tttctttatt attaactcct gtgtttgttt attttttaat tttcactccg tattttttga
     1141 gtggaaaaac actgactgat ttttatcaac tacaaaaaca aattattatt tttcagcaaa
     1201 actgggccag tccccacccc tatcaatcaa gtcctttagc gtggatgata aattggcgac
     1261 cagtttggta ttttgcagat ttgactgaaa ataattggcg gaaggatatt tttgctcaag
     1321 gtaatcccgt gcttttttta tatttcttgt ttttaccgat atttttgtgg tggtggcaaa
     1381 gatctttaaa gactaagcgc gcaataacga aatggcgcaa tctgcttgtg caaaaaacct
     1441 atcttttatt aatttgttta ttttttgctt cttttttacc atggatcgtg gtgacaagac
     1501 cgatgttctt ttatcatttt ttaccagctc taccctattt aattttaatt gttacttggc
     1561 caatctattt atttattttg agtcgtcaat ctcagcagca gcgacaactg ctactttttc
     1621 attttttgtt ttggccggtc ctaattgcca tcatctttta tccgcattgg acagccttac
     1681 cagtgcctga agcggcaagt aattatcttt actttgtcct accaagctgg cgataaggta
     1741 taatttgcta gttaagaatt ttataaaaaa catatgacaa gaattgcaat tattggtggt
     1801 ggttttggcg ccctgaatct tgcttggcga ctagcaaatc ttgattctaa agaacaattg
     1861 ggcattgata tctttgaaaa agaggcgcaa ttgggaggta tggccggtgg ttttcaagag
     1921 aaaaattggg cttggacgtt tgaaaaacat tatcatcacc tatttgccaa agacagagct
     1981 ttccaaaatt tcttgaagag tttggatttg tcagacaagc ttttttaccg taaaaccttg
     2041 accagtactc tttggcaaca gcagatttat cagttggata ctgcctggag tttgcttaat
     2101 tttacagaat taagtttcat cgatcgattg cgcactggtt tagttttagc ttttttaaaa
     2161 attataccca atggtcaatt tttagaaaaa tatcgcgcca gtgatttttt gcaaaaaact
     2221 atggggcaaa aatcttggca gattatttgg cagcctttat ttcaagccaa atttgctgat
     2281 tgggctgatg acatcaattt agcttggttc tgggccagaa ttaatccaag aacggctaaa
     2341 ttaggttatg ttgaaggtgg ctttcagagt ttagccaatc tcgttcagca aaaattagca
     2401 aaaagaggcg tgaaaattaa cctgcaatgc atagttaaaa aaattagccc tgttaaatca
     2461 acagatcagc agaattcccg atttaaacta aaatttattt caactaaaga taccgccaac
     2521 aaacaaccat ccagcaccag ccaaacccaa gaatttgact taattattaa cactctaccc
     2581 tctcctcaat ttagtcagtt aattgatttg ccagaattaa agagcgctca tttacagggt
     2641 ctggcagcga tgacgatgat tctccgactt aaagatcaac tgcttttaga taatacctat
     2701 tggttaaatg tgaatgagcg ggactggccg tttctggcag tggttgaaca tgatcatttt
     2761 gtagatcagc gtttttataa tcacgagcac ttagtttaca ttggtaagta cttatcaact
     2821 tctgatccag tctatcaatt ttctgccaaa aaattgtggc aaagttattt gccctttttg
     2881 cgccaattaa atccaaaaat tgaagagata ttaattgatt atcgtgtcac caaagaagct
     2941 tttgctcagc ccttagtgaa aaccaatcat agtcggaatt taccatcatt gcgcaccagt
     3001 taccccaatc tttattgggt atcaatgcaa cacatttatc cttttgatcg gggactcaat
     3061 caagcgatag tttctagcaa ccgcctgttt gagctaatca aaaaagattt aaaaatttag
     3121 ttgctttaca ttctagatca caagaatcgc aaattttgct atagtggtag cgatgattct
     3181 agcagtttat ttatcactac tatttttaca gttgttggct gtcccatttt tagcttgggt
     3241 tagtaagctt tttccacaaa ttaaagatgg gctgtgggcg tttggcagat atcttacctg
     3301 gttggtttta tcgctaatta tttttttcct cgctcattta aaattagctg tcaataatgt
     3361 ttccggcgtt tattttctta tttttgtgtt ggtgctggtt aattgttatt ttttgtttcg
     3421 cgccaagaac cagcaaaaac tgctgagttc aatcaaacat tattggcaac actttcatcg
     3481 ccaaattatt attgaggaag ttctatttat tttatttttt gctttcttat tagtagtgag
     3541 aagttttcaa ccagaaattt tgggtttgga aaaatttatg gatgctggtc ttatgcagtc
     3601 ttacctaaaa tctcccactt tgcccgctgg cgatatgtgg ttggctggag aaaaaattaa
     3661 ttactacagt tttggtcact ttatgggttc gattttagtg caaatctggg gaattgatat
     3721 ggcttgggct tacaacttct tgttggcgac aattatggcg attttagcta gttctgtttt
     3781 ctctttaact tttaatattc gggctaattt tacttgggga caaaaaattg gtagggattt
     3841 ttcagctaag gaaaaatata atttttcggc tacagttggg acggcgctct tagctactgt
     3901 tatggtaacg ttgtttggta atggtcatag tttgtggttt ttcttaagta atggcaatct
     3961 aactaattat ttttaccctt cagccacccg ttttattgca aacaccatcc atgagtttcc
     4021 tgtttatagt tttgtggtta gtgatttaca cgctcatgtt ttttcattac caatggtagt
     4081 ggcaatgttg atgagcattt ttctgtggtt aagtgagctg attgaggagg tgaaaagaca
     4141 aaaagtttta aagttattta cagaacggtt ttttattctc agtttagtga tgggtgtttt
     4201 tattggtgct ttggcgatga ctaatacttg ggatgttttg atttattcag tttttttatt
     4261 gattttaggg attttattac taatgtggaa aaaagaattt tggctaccat tagtgagttc
     4321 tgctctggga ttaagtttgg tagcggccat ggttagtgct ccgtggtttt taacttttca
     4381 atcaattgct gaagggatta aaattgctag tgaacactct cccctctggc aactcttggt
     4441 gttgtgggga gcgcatttga taccggtttt aatttttatt ttattgttgt tatttcatag
     4501 ccaacgacgc gaaccaaaag aagagacttc ttggtttttt gtggtagcga tgttttttac
     4561 ttttgtattt ttattgattt ttaccgaatt attttatttt aaagatattt atacaagtta
     4621 tccgcgagct aatacgatgt ttaaattgat gtttcagggt tttattctga ttgctatttt
     4681 gttagcttta gccatttcat ttttcattag tcacgccaaa accggtaaac actttaattg
     4741 gcagttgatt gatttgagag aagtttggtc acaaaaaaat aatcaattat taaaaattaa
     4801 aaccatcttg cgtactggtt tgtttgtcta tttacccatt actcttttaa ttgttggctt
     4861 agctacttat ccttatatgg cttttcgtag ctactatggg aactttcaac attatcaagg
     4921 tttaaatggc ttaaagtgga tggaaacaag atatcccagt tcttatgcga ttgttaatta
     4981 tttaaaggca aatgaagcgc agcaggttaa tattttggaa gcaagtggtg agagttttac
     5041 tgaatttagt ttaatttctg ctttttctgg aatgcccacc gttgtcggtt ggcaggtgca
     5101 tgaatggctg tggcgaggca cttgggatat accagctcag cgtttaagag agatagatgt
     5161 tatttataac aatccactta gtgctgaaag cactgcttca attgagcaat atcagctaaa
     5221 atacatcatc attactaatc gcgagcgagc aaaatttcct aatttaaatg aaaaagccct
     5281 actccagtta ggccgagtgg tgtggactgg cagtgaagac ggtattaatc aagactatct
     5341 tattttactt gaaaagtaac agtaacagtt tttgcaatgg cgcttgatcc tggttctaag
     5401 gacggactag aagagtctaa actgctggct gattcagcac ggtaaaaagg ttgataattg
     5461 gcaccgcttt caacaatttc tagtacttta actatttgtt gttgattgtt ggctgcgata
     5521 gcttcagcct tagttcgtgc gtcagcaaca gcactgttaa ttaaatcaat ttcacttaaa
     5581 ttttcttggt ctaagcgaaa attaggacca tagacgtaat tcgcttgact gttattaaga
     5641 atacttagta agtcttcact ttggctcttg agtagttcac tattctcgtt acgcaaggta
     5701 atcgtgacgc taatattggc tcgccaatct ccggttttac cagtttcatt tccttctata
     5761 agcactgttt cggcattaga taatggttga attgtgtttt catcttcttg ataaacgctg
     5821 acctgagtag tttgtatgtc tttttcacta ataccaaaat ctttgatctc tactaaaagc
     5881 tgatttaata tttcagtcgc accattaact gcctcttcct tttctggagc aatcacttca
     5941 ataccagcag taaactcagc cagttgatta aggatgttct cctgtgctga accttgaacc
     6001 gtaatggtgc ttttttctgg attgaaaaat gacgaattag ccaatggagt aaaattttgt
     6061 aataaataaa agctaacaaa acccactata aaaactacta aaaatttaaa aaaggaatta
     6121 ttattattgt tcatagattt tcattattgc ataagctgat taattttgaa acaagtttaa
     6181 ttgggcaaaa tgtcagcgga cagggtttat cagtcgacaa tcagttcggc aaatctagca
     6241 ctaacccagc cttcttgacc ttcaaatttg atttttagcc aatctgccct ctgagcactg
     6301 gaactactat ccccaccttc tgccacatcc ccaccttctg ccacatctcc accttctgcc
     6361 acatcctcat ctttgactac cttgtcatta ttttcatcat cctctggtag aactaccgca
     6421 gcgggatagt aaaaaccact tttggccaaa cccaattcgc tagctgttgg atttggtgca
     6481 gctctgaccc ttaacacttc ctgattatca acaaaaaatg ctgtttgttt aattctgacc
     6541 ttagctccag tgattgcttt tggatctgtg acgccccaac tctcgtcttg tccattgtct
     6601 atttgagcag cggcgtctat ttgagcagcg gcgctggcta ttttgttctc ttcggggcta
     6661 gtctcgactt gtgcttgatc aagagggttt tccgctaatt tagctaattt aatgctgact
     6721 ttgatggtat aaccagccaa aacttgtaga gtgtgttctt gtgtttcata ggaaggcagg
     6781 gagactcgat attggtattc ttgtggtacc aaattttcta agacaatggg ggtgtattgg
     6841 gttgtttctc cattaaaact gagtaatgcg tgttcaggat aactttcaaa actgacttgg
     6901 ccaaaatctt ttaaatcagc taccttctca atttcataaa tggtcgccgc actttctttg
     6961 gtggttggac caggtttata ggaaaccacg gtcagcgtgc cagtggttaa agtaattggt
     7021 gtactaaact ctgccaaagt ttgatcatct ggaacaattt tcaagatgta atcaccactg
     7081 cggatatctc tgtttgccag tggggctttt tctaaatatt gatcatctaa gaaaacgctg
     7141 gcgtatctgt caggacctaa atattcaatt tgtaggccag ctaaagcaat tgtatcaggt
     7201 ggattaaaat ggagaaagac agcagcacta acaatcacta tggaaaggaa aagtggccac
     7261 aatcgtttga ttatgctttg aattttcatt actacgtgcc tgtagtataa caaattccct
     7321 agtctcttag ttcaggcctt aattaagagc aatttttaac acttctttga catctgcaac
     7381 ggttttaaat tcaaggtctt ttttgacttc tttaggaatt ttaactaaat cccgttcatt
     7441 ttcctgtggg ataattacga ttttgctacc ggcacgatgg gcggcgatcg ctttttcttt
     7501 taagccacca atgcgtaaca ctctgcctcg caaagtaact tctccagtca tcgccacatc
     7561 tcgtctaacc ggttgttgct taaaagcaga aacaatagcg gtggtaatgg tcaccccagc
     7621 tgatgggcca tctttgggta ctgccccctc tgggacgtga atatgaacat cggtttgatt
     7681 aattttttct atatcaattt ttaattcttg atgatgagat ctaacataag taagagccgc
     7741 ctgggctgat tctttcatta catcgcctag tttacctgtc agtataatcc tgccttttcc
     7801 tggtgagaga gccacttcaa caaataagac atctccacct acactagtcc aagccaaacc
     7861 agtagctaaa ccaatctggt cctctttttc ggcaatggtt tcatcataaa tagctggacc
     7921 gagaaactca gttaattttt tttgattgat ggtaatgctc tttttgtttt tctctaatat
     7981 ttctctggca attttgcgca tgattttgtt gagttgtctt tcgaggtcgc ggaccccagc
     8041 ttctcgagtg tagcgactaa taattttttt cagtattgca tcactcaatt taacctgggt
     8101 ctttttcaaa gcattgttct taatggtttt gggcaataag tagcgtttgg caatttcaaa
     8161 tttttcattg agggtataac cagaatagta gattacttct aatcgatcta acagagctgg
     8221 tggtactgta tccagcgtat tcgcagtagc aataaataaa acctcagata aatcgtaagg
     8281 catatctagg taatggtctt caaagctaaa attctgctca ggatccaata cttcaagtaa
     8341 agcactacta ggatcgccat taaaatctcg acccaattta tcaatttcat cgagcataaa
     8401 cactggatta ttactctggg cattttgtaa acctttaata atccgaccag gcatagcccc
     8461 gacataagtg cggcgatgac cacgaatttc tgattcatct ctcacgcccc ctaaagaaat
     8521 tttgataaac tgacgattga gacttttagc gatacttcga ccaatactgg ttttcccaac
     8581 acccggtggt ccaacgaaac ataaaatagt gggtaaattt ttttcttggt tagcattttg
     8641 attttttaac tgcatcacag ccatgtattc caagacccga tcttttactt cttctaaacc
     8701 ataatgatct gcattaagaa ttttggctgc ccgtttgata ttaacattgg tttttttact
     8761 tttttgccag ggcaaatcaa atactaaatc caaccaactt cttaaataag aagcttctgg
     8821 attattaggc gacatttgct ctaaacgatc aatttctttt ttgacttttt tttcggcagt
     8881 ttttggtagt tttttctttt ttaattgttc ctggtaacta ttaatgatat cttgatcatc
     8941 gtccagttcg cctaattctt tttgaatagt gcgcaagcgt tcccgtaaga cactttctct
     9001 catgtgttcg tcaaactttt cttgagtctt gagattaaca tcttgctcaa tttccaaaat
     9061 tttgacttcc ttggataaat actcaattac caggcgaatt ctttgtttta catcaatagt
     9121 ttccagaata agctgttttt tctctgtagc aatgttgagg gtactggcaa tctgatcagc
     9181 catttccccc tcattaaccc cactcaataa tttaataaaa ttcaaaaatt caatttgctt
     9241 gcccatttgc acagtctgcc ggaataattt ttgcagttgg ttgctcaacg caatcaattc
     9301 gtcatcacgc tcggcaaaac tcaccatttt ttcccctatt gcttgtaaaa agggttcttc
     9361 ttgaacaaat ttttgaattt tgatacgact aattcctctt accaaagtgc tgacattgtc
     9421 ggcattctgc aaggtctttt caatgacggc taaagttccc acactataca agtctttagc
     9481 tcgcggttgt tcgacgcggg gatttctttg ggtaagtaaa ataatatggt tatcttgttt
     9541 ttgggcttgt cgaatcgctt taattgaagg ctcacggcca aaacctaaga cggattcggt
     9601 ttggggaaat aacacccctt ccctgatagg aactacaggt aaattaaaag tattgctagg
     9661 taattgaata attttactgt tcatcttttc gtttccaatt ataccctaag ttttgccaaa
     9721 aatgttgagc aattagcaga gtgcaacttt gattgctaaa tgggaatttt taaaaagtgg
     9781 gtgtgcttgg aatcggacca aggacctcag tcttatcagg actgcgctct aaccaactga
     9841 gctacacacc catttcttgt taaaaattta acaagaccat attttagcac aaatcatgtc
     9901 tttaccaaat caagcttatt tttttgccaa tcaagcctca gctggtcgcc agttttaatt
     9961 ttgcctgtaa ttatttgttc cgccaattcg tctagaattt cattctggat gagacgtttt
    10021 agcggtctgg cgccgtaaag atgatcataa ccagcctcag ctagatgttc aattaatcgc
    10081 tcactaaact ctaattgaat ttctcgttcc gctaaacgtt gggccactaa attaagttgt
    10141 aattgcacaa tcgctttaat ttctttttta cccagacttt ggaaaataat aatatcgtct
    10201 aagcggttca aaaactctgg ccggaaatgc atcttgagta agccatccag agcctttttt
    10261 tgttcaactg ggtcattgct tgtttcacta atgatttgac taccgagatt agaagtcata
    10321 atgataatac tatttttgaa attcaccgtt cggcctttgc tatcagttaa gcgtccttca
    10381 tctagtactt gtagcaaaat gttaaaaaca tcactgtgag ccttttcgat ttcatctaac
    10441 aagacaatgg tataggggtg gcggcgcagc gcttcagtta attgtccgcc ctcttcgtaa
    10501 ccgatgtaac caggtggcga gccgattagg cgagccacac tatgtttttc catgtattca
    10561 ctcatgtcaa tgcgggtgat taaatgttca tcgttaaaca gttgttctgc taaagcctta
    10621 gccagctcag ttttacccac acctgttggt cctaaaaata agaaactacc cactggtcta
    10681 ccctcttcgc taatcccagc tcgattgcgg cgaatagcgc cagccaccgc cttaatcgct
    10741 tgttcttgac cgactacccg tttcgccagt gcctcttcta aatgaatcag gcgctcgctt
    10801 tcttcagtga gcatgcgatt cactggaata cctgtccatt tggaaacaat actggcaatg
    10861 tcactttcat caatttcttc tttaagcaat ctttcttcag gatcaatact agccattttc
    10921 tgttgggctt ccttaatctt tttttccaat tcgggaattt ctccataatt gatggtggct
    10981 gctctttcca gatcataatt gcgctcagcc cttttggctt cttcccgcag ttgatccaac
    11041 tcttggttgt attgtttaat tgaatttaaa atttcttttt ctttgcgcca cctcagttcc
    11101 aaacttttgt acttttcttt taattcacta atttgcttgt taattttagc taaacgatct
    11161 tggcttttct gatctttttc ttttttgagt gactcttttt caatttccaa ctggcgaatt
    11221 ttgcgctcca actgatcaac actgctgggc ttactatcaa tttcaatccg cagagctgac
    11281 gcagcctcat ccatcaaatc gatagcttta tctggtagga agcgatcagt aatgtagcgg
    11341 tgggaaagtt ttactgccgc tacaatagcg ttatctaaaa ttttgacacc gtgatgagct
    11401 tcatatttac ttttgatccc tcttaaaatg gaaatagcat cttccaccga cggttcctca
    11461 accatcactg gctgaaagcg tctctctagc gcactatcct tttctatata ttggcgatat
    11521 tctttgaggg tggtagcacc aatagtacgt agcgtgcctc tggcgagagc aggcttgagt
    11581 aaattgccgg catcggttga accctcactg gcgccggctc ccacaatgct gtgtagttca
    11641 tcaataaata gaataatttg gccatcactt ttttcaattt ctttgatcac cgctttcagg
    11701 cgatcctcaa attcaccgcg atacttggcc ccagcgatca aagctcccat atctaaggca
    11761 agtactcgct tgtttttgag ggcttcaggg acatcatcat cgataatttt tttagctaaa
    11821 ccttccacga tagcagtttt acccgtgcct ggctcaccaa ctagaaccgg attgttcttg
    11881 gtgcggcgag acaagatctg ggtaatacgg cgaatttctt catcacgacc aatgacaggg
    11941 tcaatttttc ctgatcgtgc caaactggta aaatctaaac aatatttttc taatgcttga
    12001 tacttaccct ctggttcggg atcagttaca cgttgttgac cacgcaccgt ttttagagct
    12061 tgaactactt tttggggatc taaatccagc agttgcacaa ctttcttttg ctctaacaag
    12121 gccaaaaaca aatgttcagt actgatgtac tcatctttga gtttcacggc ttctgtttcg
    12181 gcttggctca aaactttagt taattcggca gaggcataag cttgcgtagg agtttccgta
    12241 accggaagct tttgtaattc tttcctaatt aggctggcga tggcctcagg tttggtatct
    12301 aattgttcta aaagattggg cacgattccc cccggctgtt cgaccagggc caagagtaaa
    12361 tgccagactt caatttgact atgtttcagc ttgccagcta attgcatggc atcttgaacc
    12421 gcttccgctg ctttagtggt gtattgatta aaattcataa attgtttcca gtcatttttt
    12481 aataaggtaa ttaatgatta ccttcaaggt aatcaacgat tacttcctaa ctttaatttt
    12541 agcgtgacac ggtttttttg ccaagagtca attagcagac acaagagaac tctgctaatt
    12601 tagcagtcaa tgaatttggc ttgatctatc ttatatattt tgatccactt tgatagatta
    12661 aagtaaggaa aatgatagtt aaagctggtc tcgacagcaa tttgtaatga atacggtaat
    12721 cgacaattaa ttgggaaatt ttaatacttt gatttgttgt cttcaatatt ctcaactttc
    12781 aagtgaaaac agtgtttaac agcaaatacg gtgatagaaa gtgacctggg cacctatgtc
    12841 cacaggtttt taatttttcg tttaagttac cttaaacggg agaaggaggt cgtgaaaact
    12901 cctacccatt ctccttagaa aggaggtgat ccatcggcac cttccagtac cgataccttg
    12961 ttacgactta accctcatcg ctaatctcgc cgtagaaggg ccgttctctt gcgagttacg
    13021 gtcacccgct tcgggcgcta ctaattttgc tggtttgacg ggcagtgtgt acaaggagcg
    13081 agaacgtatt caccgcaacc tgctgatttg cgattactac cgattccgac ttcatgctct
    13141 cgaattgcag agaacaatct gaactgaggg gccgtttaaa ggattggctc caccttgcgg
    13201 cttcgctacc cgttgtcggc tccattgtag gttgtgtgtt gccctggatg taaggggcgt
    13261 gctgacttga cg
//
LOCUS       DAOSEW010000008        12519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_4222_length_12519_cov_4.493341, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000008 DAOSEW010000000
VERSION     DAOSEW010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 12519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12519
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4222_length_12519_cov_4.493341"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..957
                     /locus_tag="PLJ02_00570"
     CDS             187..957
                     /locus_tag="PLJ02_00570"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOI04950.1"
                     /translation="MTFWQAILLGLVQGLTEFLPVSSSGHLFLLENFFQTSSGSLSFE
                     ITLHLATLLATIIFFWPSIKNISKKTILLIAVATSPLVLVVFFINDFLTEVSQSVSWI
                     AFFLSISALLNFFSFLILNKKDDKPKVTQLENISFLTSLKIGLLQVLAVFPGVSRSGM
                     TLFASLSNKIQAETAFKFSFLLSIPTILGALVFDLINGGATNLQTINWPITVSAMLVA
                     FCSGLFSLKLLKGVLNRNRFLFFAGYCLFLSVGLLLFI"
     gene            complement(950..2473)
                     /locus_tag="PLJ02_00575"
     CDS             complement(950..2473)
                     /locus_tag="PLJ02_00575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOI04951.1"
                     /translation="MFVKTYSAINLALNVKTIEVEVDTVIGKPSFVLIGLANRAVEES
                     KERISAALMHCGVRIKPLRTVVNLAPAELKKTSSALELAIAVALLLQYGEINIHVQQA
                     LFLGELSLTGELKAVKGILGIALQAKSLGFSQLYFPEANLSELLLIKDLELFPLSSLA
                     EFISLAKKNSLKQLTSKINHFQNLATQSEVSNIPDLAEFQEQDQAKRALSLCAAGGHH
                     LLLFGEPGAGKSYLAQSILSILPDLSDEEILELSQIYSLLAPLKAGLISQRPFRQPHH
                     SVSKAAFLGGLDFPGEISLAHLGILFLDEFTELRRDLIEALRQPLENQFFKINRAKQE
                     LIYPANFTLIAAANPCPCGFYASDKPCRCSTYDLIRYQKKLSGPILDRIDLSIRLKAS
                     SQRELFFASDSAQTSQRIKEQVQLAKLKQVQRFKNSPFKSNANLTSEGVKQFCQLSKA
                     AKKTLDLARTQLGLSVRAYFKTIKIAQTITDFQDEKMISTQAIAEALSFRQTPINPR"
     gene            2555..3310
                     /locus_tag="PLJ02_00580"
     CDS             2555..3310
                     /locus_tag="PLJ02_00580"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOI04952.1"
                     /translation="MFATFLYQVKKPYHFLKTALFKAVPAQFKYKFPQKKLKIIAITG
                     TDGKTTSTTLLYHLLKTAGYQTAMVSTVAACIGKEEIETGFHVTSPPPDNLYRLMRQM
                     VNENIEYLILEYTSQGAYQYRLFGVRPLIAGVTNINRDHFDYHLNYENYLNAKASIMK
                     KSKIVVLNEDDQSFYRLKRILPSSKHLVLTYSKEDPLNQPLEEALLERFPEEFNQMNA
                     RLAISIAQKIGVDETQLIAGLKIFQESVAEWNF"
     gene            3298..3789
                     /locus_tag="PLJ02_00585"
     CDS             3298..3789
                     /locus_tag="PLJ02_00585"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOI04953.1"
                     /translation="MEFLDLKSLSDHHQPFQVVVDFAHTPQGLRESLTALRQKMKNDR
                     LSGRLIAIYGCAGLRDREKRPMMGKIGAELADLVIFTAEDPRTENVWNIIRQMKEALG
                     NNHHKVISIADRQEAINFALRKIAKKGDLIALLGKGPEKSMCYGTVEKPWSEIEAVKK
                     ALK"
     gene            3798..5297
                     /locus_tag="PLJ02_00590"
     CDS             3798..5297
                     /locus_tag="PLJ02_00590"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOI04954.1"
                     /translation="MSEKLAAQKEATSLLNYQNFYLVGIKGVAMTSIAQCLLDAGKNV
                     RGSDVKEDFVTAKILREKAITVESFAEDFLSSTNGNRPDCLIYTAAHQGIDHPQVKKA
                     IANKIPVFSQAAALADLFNQQEGVAVCGVGGKTTITAMIIWVLKQLNIPISFSLGVGE
                     IIGLKATGAWNSQATYFIAEADEYAVDANALKKGKKFQARFSFLKPALVVCSNLSYDH
                     PDVYASFSDTKNTFKKFFLQIKENGCLIINSDNEALVKLAQEVKTIRNDLKIVSFAWA
                     QPADYFLSHYQIQNGHSLATVNQRLNNNLRQAPLRLKVPGQFNLMNSLAAVAALAELQ
                     VPLQQSCQALGKFQSTKRRFELVKIANGQRFFDDYAHHPHELSELIRTLNQYFSTNTK
                     LIAFQPHTYSRTKALFKDFVEVLGSNLQETDQLLILDIFASAREKKDDSISSQALVKA
                     IQDKFPQCKVLYLKNLEALASYIKKNKFDLTITVGAGDIYKVYELLSFS"
     gene            5330..5413
                     /locus_tag="PLJ02_00595"
     tRNA            5330..5413
                     /locus_tag="PLJ02_00595"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5364..5366,aa:Leu,seq:taa)
     gene            5438..5511
                     /locus_tag="PLJ02_00600"
     tRNA            5438..5511
                     /locus_tag="PLJ02_00600"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5472..5474,aa:Pro,seq:tgg)
     gene            5658..7214
                     /locus_tag="PLJ02_00605"
     CDS             5658..7214
                     /locus_tag="PLJ02_00605"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HOI04955.1"
                     /translation="MTDQQLIQLAKQSLQKNFGFADFRPGQLDIVLSILKKQNTLAIL
                     PTGGGKSICFQVPGLIFPGLSIIISPLISLMKDQVDHLLAKNISATFINSQLDKKTIE
                     QRLAALANHQYKFLYLAPERLTNENIINLCKTINISMICVDEAHCISMWAHQFRPAYK
                     KIPEFLEKIQKNQEKIVISAFTATATALVKKEIKQLLKLTPCQEFSASFLRENLIFHN
                     LTCFSEAEKNIYLLKILKIHQEKNAVIYCSTRAACEKLCRLLTTLKPNVAIGFYHGGM
                     EKAARQNIQDQFLSGQLQIMIATNAFGMGVDKADVHLVVHYQVPANLENYYQEAGRAG
                     RDGQLSYCYLLYADKDLYIQKTLLDKSYLNQQENPRYQIELQKLKTMRNYARSKTCLE
                     REIEAYFTDPQTTKQTANRKNSQSTKKNCNHCYLCLNLQLYLDQEELNTIQQLLTANQ
                     ILTKQLSEKFSIFNQQAFLLPSVFHLQSIEALAITQQKISQQSIIPGIGTGLNAVLNQ
                     LKFSPITPDQ"
     gene            7229..7564
                     /locus_tag="PLJ02_00610"
     CDS             7229..7564
                     /locus_tag="PLJ02_00610"
                     /inference="COORDINATES: protein motif:HMM:NF024057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HOI04956.1"
                     /translation="MQLDKIIDEYKKISQFLNTRWPLKDEKQRIFARTMKIVEELGEL
                     SDEILTSMNLQRNSKIANFSRENLEDEFADVMGSLILLGIELNIDIGQVVERKITYTR
                     QRLEMDKKD"
     gene            complement(7568..9370)
                     /locus_tag="PLJ02_00615"
     CDS             complement(7568..9370)
                     /locus_tag="PLJ02_00615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HOI04957.1"
                     /translation="MSQIKNQELIFELVKKLETNLNLSWPKLLSIIKKYPKKDGGFFS
                     KTEIINAYRQLYPHKPYGKLVQKLRLKPTRSASGVAPVTVLTKPYPCPGNCLFCPNDL
                     RMPKSYLAEEPGAQRAEKNFFDPYLQVMSRLTALADMGHQTDKVELIVLGGTWTNYPE
                     NYRLWFIEQCFKALNDFGNKDRTNTIRRRYELAIKKLDQPFMSSEAQENLTLLKKLDL
                     DEEQVRAQYNQLITKHYLLPEKKAGISDWQNSNWQKLAIAQKINETASSRCVGLVLET
                     RPDEITQHSAIDLRRLGATKIQLGVQALDDQILKLNRRGHGVAETKQAFAILRQLGFK
                     LHVHWMANLYGRTVKEDQQDFLKLFTSKDLRPDELKIYPCSLLETAPLMQYFKEGKWR
                     PYDYAELLAITTFTLKHTPAYCRITRMLRDIPAQDIVVGNVKSNFRQIAEAQVKKEGV
                     KIKEIRAREIRRQVFDKQKIKFSTITYQTSVSQEYFLQFTVKVETGEEKILGFLRLSL
                     PKEKNHWYSELTDSAVIREIHVYGQALALGSEAQGRAQHLGLGSQLIDEAKKIAKEAN
                     FAKLAVISAIGTKEYYRSRGFKDGELYQFFALKQ"
     gene            9422..10240
                     /locus_tag="PLJ02_00620"
     CDS             9422..10240
                     /locus_tag="PLJ02_00620"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOI04958.1"
                     /translation="MQQETIKMLNQLNRDFYYRVADDFSESRQYYWPGWQKIPLFFKK
                     NQQNAKKIQVLDVGCGNARFAKFLKKNNFNFNYIGIDNSAKLLKIAQDTLQKEAISAE
                     LIEFDLIDHYLANQQITWPVAEKFDLIVVFGLTHHLPSAQLRLQFFQSLAKLLKKDAL
                     LVVSNWQFAVDKRFQKNIISRQKNQPTSKINTSPIIKLQKILNKLEKNDYLLDWRQTT
                     QKSNGDTSIRYCHYLDEKNSKILFKKADLKIVDQFFADGKSQRLNQYFVLKKAN"
     gene            10267..10337
                     /locus_tag="PLJ02_00625"
     tRNA            10267..10337
                     /locus_tag="PLJ02_00625"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10299..10301,aa:Gln,seq:ttg)
     gene            10363..10992
                     /locus_tag="PLJ02_00630"
     CDS             10363..10992
                     /locus_tag="PLJ02_00630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOI04959.1"
                     /translation="MLTNDRVDLLNKIPNDKNIKAILFDLVGVLVFKKEDYIPTTPDE
                     INAQNIESLFNHLDDKKLIKDVKEKLKLSDDEIQTAVRLIPEKYEKFDELWKLLPELK
                     KKYKLVVINNGNAITKSYWHKKFDFSIFDLFLNSAEERIKKPDPKIFLLACAKLKVKP
                     EECLFMDDSLENIKSAQKLKMTTIWWNKEADKTALIQKLKETVNISPEK"
     gene            11083..11547
                     /locus_tag="PLJ02_00635"
     CDS             11083..11547
                     /locus_tag="PLJ02_00635"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOI04960.1"
                     /translation="MEDKKFLQLAIKQARQSMEQGGFPAGAVVVKDGKVIAEGISLGY
                     QLHDPTEHAETSAIRKACQLVGSSNLEGATLYESLECCNMCFSVAVWGGISRVVYACR
                     KTPEMVKKFYYEGMTKNEDINNTNNTKIELIFIPDYEQECLDIVREWEEKQK"
     gene            11526..12512
                     /locus_tag="PLJ02_00640"
     CDS             11526..12512
                     /locus_tag="PLJ02_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04961.1"
                     /translation="MGRKTKIILKEQELIEIIPSSPFDFDSTFHKPDHFTSGDNHWEP
                     GIKWQTWLWENNSIGLKIFNKGTREKPKLNIEIYSNKKLSKDFINSLIEEIKYRYNLN
                     LDLSNFYHAFAKDKTLGPIIKKWRGMRPGHPSSLYEYLLIGIVLQNATVKRSVQMFRA
                     LLENYGTLLKFDGKELRCFFKPGSLQTVSEDELRALKLGYRAKSIKRIDDYFAQGLMD
                     EMALRKMNRETQMNELLKLYGVGPATVWYLLFDVFHHWDFFNHVSPWEQKIYSKIFFD
                     VDPEQPVSVKKILKYFERFGDYKQLAVHYIWEDLWWKRKNENIPWLEKEIRI"
BASE COUNT         4095 a         2304 c         2276 g         3844 t
ORIGIN      
        1 tctgtttgaa tagtaatgat ttatctaggc gcaacgactt tgaccttaat ggatctactc
       61 cctatatttc cttcgggaaa gatggtcata aagttgcgcc tagataaatc attactattc
      121 aaacagaatc cggcttatga gccatgttca ctgttaatta ttgctatttg tgcgtagaat
      181 agaggcatga ctttttggca agcaattctc ctaggtttgg ttcaaggatt aactgagttc
      241 ttaccagtct ctagttctgg acacttgttc ttgcttgaaa atttttttca aaccagctcg
      301 ggttctttaa gctttgaaat cactctgcat ttggccactc tactagccac cataattttt
      361 ttctggccat ctatcaaaaa catttctaaa aagacaattt tactgattgc cgtggccact
      421 tcacctttag tcctggtcgt cttttttata aacgattttc tgacagaggt tagtcaatca
      481 gttagctgga ttgctttttt tctctcaatt tcggctttac tcaatttttt tagtttctta
      541 atactgaata aaaaggatga caagccaaaa gtaactcaat tggaaaacat tagttttctg
      601 actagtctca aaattggtct gttacaagtg ctggccgttt ttcccggagt ttctcgatcg
      661 ggcatgactc tgtttgcttc actgagtaat aaaattcaag ccgaaacagc ttttaaattt
      721 tcatttctct tatcaattcc cactattctg ggagctcttg tttttgactt aattaacggt
      781 ggcgcgacca atttgcaaac aattaattgg cccataaccg tatcagccat gctagttgcc
      841 ttttgcagtg gcttgtttag tctaaaacta ctcaaggggg tcttaaatcg caatcgcttt
      901 ttattttttg ctggttattg tttattttta agcgttggtt tattactttt catctaggat
      961 taatcggcgt ttgacgaaaa ctaagggctt cggctattgc ttgagtagaa atcatttttt
     1021 catcttgaaa atcagtaatt gtttgcgcaa ttttgattgt tttaaaatag gcgcgcacac
     1081 taagtcccag ctgtgttctg gctaaatcta aagttttttt ggctgcttta cttaattgac
     1141 aaaattgttt aaccccttca ctagttaaat tagcattaga tttgaatggg ctatttttaa
     1201 agcgttgaac ttgtttgagt ttagctaatt gtacttgctc tttaattctt tgactggttt
     1261 gtgctgagtc agaggcaaaa aataattcac gctgagaact agctttgagg cgaatactta
     1321 gatcaatccg atccaaaatt ggacctgata attttttttg atagcggatc aaatcatagg
     1381 tgctacaacg acaaggttta tcactggcat aaaaaccaca gggacaaggg ttggccgcag
     1441 caattaaggt aaaatttgct ggataaatta attcctgttt tgctcgatta atcttaaaaa
     1501 attgattttc taagggttgt ctgagagcct cgatgagatc ccgacgcaac tcagtaaatt
     1561 catctaaaaa taaaatgcct aggtgggcta aactaatttc accaggaaaa tccaaaccgc
     1621 ccaaaaaggc agctttactg acactgtgat ggggttgccg aaacggccgt tggctaatta
     1681 atccagcttt caggggcgca agaagtgagt aaatttgact tagctcgaga atttcttcat
     1741 cacttaaatc tggcaaaatt gataatatac tttgggctaa atagcttttt ccagcccctg
     1801 gttctccaaa aagtaataaa tgatgacctc ccgctgcaca cagacttaaa gcccttttcg
     1861 cttgatcctg ttcttgaaat tcagctaaat ctggaatatt acttacttca ctttgagtcg
     1921 ctaaattttg gaaatgatta attttgcttg ttaattgctt tagcgaattt tttttagcca
     1981 aactaataaa ttcggccaaa ctagataaag ggaagagctc cagatcttta attaacaata
     2041 attcacttaa atttgcttct ggaaaataca attggctaaa acccaaactt ttagcctgca
     2101 aagcaatacc taaaatccct ttaactgcct ttaattcgcc agtcaaggac agctcaccca
     2161 aaaacaaggc ttgctggaca tggatgttga tttcaccata ttgtaggagg agtgctaccg
     2221 caattgctaa ttccaaagcg ctgctagttt tttttagttc tgctggagcc aaattgacca
     2281 ccgttctcag tggtttgatc ctaaccccac aatgcatcag ggcagcgcta attcgttctt
     2341 tggattcttc caccgcccgg ttagctaaac caattaaaac aaagctgggc ttgccaatca
     2401 ctgtgtcaac ttctacttca atagttttga cattaagagc taaattaatc gcactataag
     2461 ttttgacaaa catctttttt aattgtcgct gagaaatatg acaaatctag ctattattta
     2521 gaaattgtga ctaaagtgtt atcatagaaa caacatgttt gctacttttt tatatcaagt
     2581 aaaaaaacct tatcattttt tgaaaaccgc tttgtttaaa gccgtgccag cccaatttaa
     2641 atataaattt cctcaaaaaa aactaaaaat cattgctatt actggtaccg acggtaaaac
     2701 tactagtacc actttacttt atcatttatt aaaaaccgct ggctatcaaa cagccatggt
     2761 ttccaccgtt gccgcttgta ttggcaaaga agaaattgaa actggttttc atgtcactag
     2821 tccacctcca gataatcttt atcgcttgat gcgacagatg gtaaacgaaa acattgagta
     2881 cttaatttta gagtacactt cgcagggagc atatcaatac cgtttgttcg gtgttcgccc
     2941 actaattgct ggtgtaacca atattaaccg tgatcatttt gactatcacc ttaattatga
     3001 aaattactta aatgctaaag cctctatcat gaaaaaaagt aaaattgtgg tcttaaatga
     3061 agacgatcaa tctttttatc gcttaaagcg aatattaccc agctccaaac atctggtgtt
     3121 gacctatagc aaagaagatc cacttaatca accactagaa gaagcgttac ttgaaagatt
     3181 tcctgaggaa tttaaccaaa tgaatgctcg tctagcaatc agcattgccc aaaaaattgg
     3241 cgttgatgaa acacaattaa ttgctggatt aaaaattttc caggagtcag tggcagaatg
     3301 gaatttttag atctaaagag cttaagtgac caccaccaac cctttcaagt cgttgttgat
     3361 tttgctcata ctcctcaggg tttaagagaa tcgctaaccg cgctgagaca aaaaatgaaa
     3421 aatgatcgac tcagcggtcg cttaattgcc atctatggtt gcgcaggtct tcgagatcgg
     3481 gagaaaagac caatgatggg taaaattggt gctgaactag ccgacttagt aatttttact
     3541 gccgaagatc cccgcaccga gaatgtctgg aatattattc ggcaaatgaa agaagcactg
     3601 ggcaacaatc accacaaagt gatttccatt gctgaccgtc aagaagcgat taatttcgcc
     3661 ttgcgaaaaa ttgccaaaaa gggggatttg attgctcttt taggtaaggg accagaaaaa
     3721 agcatgtgtt acggcacagt cgaaaaacca tggagtgaaa tagaagcagt caaaaaggct
     3781 ctcaaataaa ctgccctatg tctgaaaaac ttgcagcaca aaaagaagca acttctttac
     3841 tgaattatca aaacttttat ttggtgggta ttaagggcgt cgccatgacc tcgattgctc
     3901 aatgtttatt ggatgcaggg aaaaatgtca gggggagtga tgttaaagag gactttgtca
     3961 ccgctaagat tttacgggaa aaagcaatta cagtcgagag ttttgccgaa gattttttat
     4021 caagcacaaa tggcaataga cccgactgtt taatttatac tgctgcccat cagggtatag
     4081 atcatcctca ggttaagaaa gcgattgcca acaagattcc ggtattttct caagcagcag
     4141 ctctcgctga tttatttaat caacaagaag gagtggctgt gtgtggcgtc ggaggtaaaa
     4201 ctaccattac tgccatgatt atttgggtgt taaaacaatt aaatatcccc atttccttct
     4261 ccttaggggt gggtgaaatt attggtctca aagcaaccgg agcatggaat tcacaagcca
     4321 cttattttat tgccgaagca gatgaatatg cggttgacgc gaatgctttg aagaagggaa
     4381 aaaagttcca agcccgattt agttttttga aaccagcgct ggtggtttgt agcaatctca
     4441 gctatgacca tcctgatgtc tatgccagtt tcagcgacac taaaaacact tttaaaaagt
     4501 tttttttaca gatcaaagaa aatggctgtt taattatcaa tagcgataac gaagcactag
     4561 tcaagctggc gcaagaggtc aaaaccataa gaaacgatct taaaatcgtt tcttttgctt
     4621 gggcccaacc cgctgattat tttttatcac attatcaaat tcaaaatggg cacagcttag
     4681 ctacagttaa tcaaagatta aataataacc tccgacaagc gccactcaga ttaaaagttc
     4741 ctggtcagtt taatttaatg aactctctag ccgctgttgc cgccctggcc gaattacaag
     4801 ttcccctgca acaaagttgt caggctttag gtaaatttca atcaactaag cgtcgttttg
     4861 aattagtcaa aatagcgaac ggtcaacgtt tttttgacga ttacgctcat cacccccatg
     4921 aattaagtga attgattcgt actctcaatc aatatttttc cacaaataca aagctgattg
     4981 cttttcagcc tcacacttac agtcgcacca aagcgctgtt taaagatttt gtggaagtgc
     5041 tgggaagcaa tcttcaagaa actgatcaat tactaatttt agatattttt gcttctgcta
     5101 gagaaaaaaa ggatgatagt atttccagtc aagccctagt gaaagctatc caagataagt
     5161 ttcctcaatg taaagtttta tacctgaaaa atcttgaagc attagctagt tatattaaga
     5221 aaaataaatt tgatttgacg attacggtag gagctggaga catttataag gtctatgaac
     5281 tcttgtcttt tagctaataa aaaatggtat aatttgagag ttaacagtcg ccgcggtggc
     5341 ggaattggta gacgcgcgag acttaaaatc tcgtactagc aatagtgtgt gggttcgatt
     5401 ccctcccgcg gcactttgac aggacagtta ctaacagcgg ggaatagctc agatggctag
     5461 agcgtacggt ttgggaccgt aaggccgcag gttcgagtcc tgtttccccg acaaagtaag
     5521 cagtcaaacc cacaatcaac attcttaaac cagcactaaa atcaattttt taagaagttt
     5581 ggcacaagaa tatttctcaa ttcaccaaga tttaattttg tcacttattt gtggagctta
     5641 tcgtcaataa tgagccaatg accgatcagc aattgataca attagccaaa caaagtttac
     5701 aaaaaaattt tggatttgcc gatttccgcc cgggccaact ggatattgtt ttatcaatct
     5761 taaaaaaaca aaacactttg gctatcctac cgactggcgg tggtaaatcg atttgctttc
     5821 aagtgcctgg tttaattttt ccaggcctta gcattatcat ttcaccctta atttctttaa
     5881 tgaaagatca agttgatcat ttactggcca aaaatattag tgcgaccttt atcaacagcc
     5941 agcttgataa aaaaacaatt gaacagcgac tggcagcctt agccaatcat caatataaat
     6001 ttctttacct agcaccagag cgactgacta atgaaaacat cattaacttg tgcaaaacca
     6061 ttaacatttc tatgatctgc gttgatgaag cgcattgtat tagcatgtgg gcgcaccaat
     6121 ttcgtcccgc ttataaaaaa attccagaat ttttagaaaa aattcaaaaa aaccaggaaa
     6181 aaattgtcat cagtgctttt accgctacgg ctactgcttt ggttaaaaaa gaaattaaac
     6241 aattactaaa actaacgcca tgtcaagagt ttagtgcgag ttttttgcga gaaaatctga
     6301 ttttccacaa tctgacttgt ttcagcgaag ctgaaaaaaa tatttatttg ctgaaaatct
     6361 taaaaatcca tcaagaaaag aatgcggtca tttattgcag tacgcgcgcg gcctgtgaaa
     6421 aactttgtcg gctcttaacg accctcaagc ctaatgtagc aatcggcttt tatcacggcg
     6481 ggatggagaa ggccgcgcgc caaaacatcc aagatcaatt cttgtcaggc caattgcaaa
     6541 ttatgatcgc taccaatgct tttggtatgg gagtggataa agctgatgtg cacttggtgg
     6601 ttcattacca agtaccagcg aatttggaaa actattatca agaagcgggc agggcagggc
     6661 gtgatggtca actgagttat tgttacttac tttacgccga taaagatcta tatattcaaa
     6721 aaactttgtt agacaaaagt tatctcaatc aacaagaaaa tcctcgctat caaattgaac
     6781 tgcagaaact caaaaccatg agaaattatg ctcgcagtaa aacttgttta gaaagagaaa
     6841 ttgaagctta ttttaccgat ccacagacta caaagcagac tgccaacaga aaaaatagtc
     6901 agtcaacgaa aaaaaattgt aatcattgtt atctttgttt aaatttacag ctttatctcg
     6961 atcaagaaga attaaatact attcaacaat tactaacagc caatcaaatc ttaacaaaac
     7021 aattgtcaga aaaattctcc atctttaatc aacaggcttt tcttttaccc agtgtttttc
     7081 acttgcaaag catcgaagct ttggccatta ctcaacaaaa aatcagtcag caatccataa
     7141 tccccggtat tggcactggc cttaacgctg tcctcaatca actcaagttt tcaccaatta
     7201 ctcctgatca atgatataat aaccagttat gcaattagat aaaatcattg atgaatacaa
     7261 aaaaattagc caatttttaa atacccgttg gccactcaaa gacgaaaagc aacgcatctt
     7321 cgccagaaca atgaaaattg tggaagagtt gggagaattg tctgatgaga ttttaactag
     7381 catgaatttg caacgtaatt ccaaaattgc taacttttct cgtgaaaatt tggaagacga
     7441 gtttgccgat gtgatgggtt cattaatttt gcttggtata gaactcaata ttgatattgg
     7501 acaggtggta gagcgaaaaa ttacttatac gcgtcagcgt ctggaaatgg acaaaaaaga
     7561 ttaattgtta ttgctttaaa gcaaaaaatt gatatagctc gccatcttta aaaccccgag
     7621 atcgataata ttctttagtt ccaatcgccg aaataactgc taatttagca aaattggctt
     7681 ctttggcaat ttttttggct tcatcaatca attgactacc aagacccaag tgttgcgctc
     7741 gaccttgcgc ttcgctacca agagccagag cttgcccata aacatggatt tctctaatca
     7801 ccgcactatc tgttaattca ctgtaccaat gatttttttc tttgggtaaa ctgagtcgca
     7861 aaaatcccaa aattttttct tcaccagttt caacctttac cgtaaattgt aaaaaatatt
     7921 cctgactaac cgaagtttga taggtaattg tgctgaattt aattttttgc ttatcaaaga
     7981 cttgccgacg aatttctctg gcacgaatct ccttaatttt gaccccctct tttttgactt
     8041 gcgcttcagc gatctgccgg aaattacttt tgacattacc aacaacaata tcttgagctg
     8101 gaatgtctcg tagcatcctg gtgatccgac aataggccgg agtatgttta agagtaaaag
     8161 tggtaatcgc caacaactct gcataatcat aaggtcgcca cttaccctct ttaaaatatt
     8221 gcattaaagg ggcagtctcg agtagagaac agggataaat ttttagttcg tcaggacgca
     8281 aatctttact ggtaaataac ttcagaaaat cttgctgatc ctctttgacc gttctgccat
     8341 ataaattagc catccaatgg acatgaagct taaatccgag ttggcgcaaa atagcaaagg
     8401 cctgttttgt ttcagccact ccgtgcccac ggcgattcag ttttaaaatc tgatcgtcta
     8461 gagcttgtac ccctaattgg attttagtag caccaagtct gcgtaaatct atagcgctgt
     8521 gttgcgtaat ttcatctggc ctagtttcta acaccaagcc gacacaccga ctgctagcag
     8581 tttcatttat tttttgggca atagctaatt tttgccaatt agaattttgc caatcactaa
     8641 tcccagcttt tttttctggt aataaatagt gtttagtgat caattgattg tattgtgctc
     8701 gaacctgctc ttcatctaaa tcgagttttt ttaacagagt taaattttct tgagcctcac
     8761 tactcataaa tggttgatct aattttttga tcgccaactc atagcgccga cgaatagtgt
     8821 ttgttctatc tttgttgcca aaatcattta gtgctttaaa gcattgttca ataaaccaca
     8881 aacgataatt ttcaggataa ttagtccaag ttccacctaa aacaattaat tctactttgt
     8941 cggtttgatg acccatatcg gctaaagcag ttaaacgact catcacttgt aaataaggat
     9001 cgaagaaatt tttttctgct cgttgggccc ctggttcttc agccaaataa ctttttggca
     9061 tccgcaaatc attgggacaa aataaacaat ttccaggaca gggatagggt ttggttaaaa
     9121 ctgttactgg agccacgcca gaagcactac gcgttggctt gagtcgcagt ttctggacta
     9181 gtttgccata aggtttatgg ggataaagct gccgataagc attaataatt tctgttttgc
     9241 taaaaaagcc tccgtctttt ttgggatatt ttttaatgat gcttaatagt ttcggccaag
     9301 ataaatttaa attagtttct agttttttca ctaattcaaa tatcaactct tgatttttga
     9361 tttgagacat taagctttat tataccctgc aattaatttt tatttgctaa aatagactag
     9421 catgcaacaa gaaactatta aaatgctcaa ccaactcaac cgagactttt attatagggt
     9481 ggcagatgat tttagtgaga gtcgacaata ctactggcca ggctggcaaa aaattcctct
     9541 tttttttaaa aaaaatcaac aaaatgccaa aaaaattcaa gtgttagatg ttggttgtgg
     9601 taacgcccgt tttgccaaat ttcttaaaaa gaataatttt aattttaatt acataggcat
     9661 agataattca gccaaacttt taaaaattgc gcaagatacc ttacaaaaag aagcgattag
     9721 tgctgaactg atcgaatttg atttgattga tcattattta gcaaatcaac aaattacttg
     9781 gccggttgct gaaaaatttg atctgattgt tgtctttggc ctgacacatc acttgcccag
     9841 tgctcaattg cgcttacaat tttttcaatc attagccaaa ctgctaaaaa aagatgcttt
     9901 actagttgtc agcaactggc aatttgccgt agataagcga ttccaaaaaa atattattag
     9961 tcgtcaaaaa aatcaaccaa ctagcaaaat taatacttcg ccaatcatta aactccaaaa
    10021 aatattaaat aagctcgaaa aaaatgatta tttactggac tggcgtcaaa caacacagaa
    10081 atcgaacgga gatacaagca ttcgttattg tcattatcta gatgaaaaaa atagcaaaat
    10141 tttatttaaa aaagctgatt taaaaatagt cgatcaattt tttgctgatg gtaaatctca
    10201 gcgacttaat caatattttg ttttgaaaaa agccaattaa acaattataa taacaggctg
    10261 taacaatgcc cgatcgtcta atggcaggac agaggccttt gaagccttta atcatggttc
    10321 gaatccatgt cgggcatccc ttttttaggc acaaattaag ctatgttgac gaatgataga
    10381 gtagatttgc tgaacaaaat tcctaatgac aaaaatatta aagcaattct ttttgaccta
    10441 gtgggcgtac tggtttttaa gaaagaggat tatattccaa caactccaga cgaaattaat
    10501 gctcaaaata tagaaagtct atttaatcat ttagacgaca agaaactaat taaagatgtt
    10561 aaggaaaaac tgaagttgag cgacgatgaa attcaaacgg ctgttcgttt gattcctgag
    10621 aaatacgaaa agtttgatga gttgtggaag ttgcttcccg aactgaaaaa aaaatacaaa
    10681 cttgtagtta tcaataacgg gaatgcaatc actaaatcat actggcataa aaaatttgat
    10741 ttcagtatct ttgatctatt tttgaactct gctgaagaga gaattaaaaa accagacccg
    10801 aaaatatttt tattagcctg cgccaaatta aaagttaaac cagaagagtg cctattcatg
    10861 gatgattcat tagaaaatat caaatcggct caaaaactaa aaatgaccac tatttggtgg
    10921 aataaagaag cagataaaac ggctttaatc caaaaactaa aagaaacagt caatataagt
    10981 cctgaaaaat aaagctatcg gcaaaatctc ttcgcggtgg taagcatctg cactccttca
    11041 gacgtaggca ataaggataa tttttgtgct aaaataactc taatggaaga taaaaagttt
    11101 ttacaattag ccattaagca agccagacaa tctatggaac aaggtggttt tcctgctgga
    11161 gcagtagtgg ttaaagatgg aaaagtaatt gcagagggga taagtctcgg ttatcaactt
    11221 catgatccta cagaacatgc ggaaacttca gcgattagaa aagcttgcca attagttggt
    11281 tcttcaaatt tagaaggagc aactctttat gaatcgttag agtgctgtaa tatgtgcttc
    11341 tcggttgcag tctggggagg aatttcaaga gtagtttacg cctgcaggaa aactccagaa
    11401 atggtgaaga agttttatta cgaggggatg acaaaaaacg aagatataaa taatacaaac
    11461 aacacaaaaa ttgaacttat ttttattcca gattatgaac aggaatgttt agatattgtt
    11521 agagaatggg aagaaaaaca aaaataatcc taaaagaaca agagttaatt gaaattatcc
    11581 catcttctcc ttttgatttt gattcgactt tccacaaacc agatcacttc acatcaggtg
    11641 ataatcattg ggaaccggga ataaaatggc aaacttggct gtgggaaaat aacagtattg
    11701 gactaaaaat tttcaataaa ggaaccagag aaaaaccgaa gttaaatatt gaaatttatt
    11761 caaataaaaa acttagtaaa gattttatta attcattaat tgaagaaatt aaatatagat
    11821 acaacttaaa tttagattta agtaattttt atcatgcatt cgcgaaagat aaaacattgg
    11881 gaccaatcat caaaaaatgg cgaggaatga gaccaggaca tcccagttcc ctttatgaat
    11941 atctgttaat tggcattgtt ttacaaaatg ctacggttaa aagatctgtc caaatgttta
    12001 gggcacttct tgaaaactac ggaacattat taaaatttga tggcaaagaa cttaggtgct
    12061 tctttaaacc tggaagttta caaacagttt ccgaggatga attaagagca ttgaaactgg
    12121 gttatcgggc aaagtcgata aagagaatag atgattattt tgcacaaggg cttatggatg
    12181 agatggcatt aagaaaaatg aatagagaaa cacaaatgaa cgaactatta aaattatatg
    12241 gggttggtcc agctactgtt tggtatttat tatttgatgt ttttcatcat tgggattttt
    12301 ttaatcatgt atctccttgg gaacaaaaaa tatattctaa aattttcttt gacgtcgatc
    12361 cagaacaacc agtgtctgtt aaaaaaatac ttaaatactt cgaaagattt ggagattata
    12421 aacagctggc ggttcattat atatgggaag atctgtggtg gaaaagaaaa aatgagaata
    12481 taccatggtt ggaaaaagag ataagaatct aaattggca
//
LOCUS       DAOSEW010000009        11616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_4660_length_11616_cov_4.334141, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000009 DAOSEW010000000
VERSION     DAOSEW010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 11616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11616
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4660_length_11616_cov_4.334141"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..794)
                     /locus_tag="PLJ02_00645"
     CDS             complement(186..794)
                     /locus_tag="PLJ02_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04962.1"
                     /translation="MTLTRYYCCFDEQQQSYQLITCRQFPKKKWKGVIPLDLPLDRVA
                     DCNQFLEQALQQELIKLRKEVFFQKFWQLADFQTQRSLLLSIYLRQKYPRGVFNVEIG
                     NWLNTGPFAQIYQQNKKLLKNLKPRQILSRLIVTSGNHKKSLRLGHIHLAYDRIKGQD
                     IFSIHYDKHNPNHLKDLGLLKHFFFEDGKNKNLLKVFNKQDY"
     gene            complement(797..2248)
                     /locus_tag="PLJ02_00650"
     CDS             complement(797..2248)
                     /locus_tag="PLJ02_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04963.1"
                     /translation="MQEQAFALLYQDKKLLENTKVPIITLSASFKEDLKGFHGEPEND
                     QIQDIVFSRAHYSMALAVAVAAWGNKIDAKKAWLVDPTNHIKKSDWKQVILTDTIGRT
                     VARQPLLKILKDVVDRFGRSKLPILENIEPATKFLTQNLDRPILSFHIATGNILAANG
                     KQVLQMITDPHVRSEYVQNADQANIRFMVFDEKTKEDFFSVAAAEQIKIPAEQIQDKV
                     IVTGPPVDPRTLAAAQSKKPWTNERPLKILITTGGLGTNKQEIKKILDQLLPLLNHVA
                     TDLPKIEIMYYAGTHADHQQMAVTLAKKHHIKYHLISPHDPADFTQAGWLNRAHRQPQ
                     SQIKFHIIYHPQIIDANELLIRYGFPWADVFISKPSGDMAYDAALSGSALLTLKEWGE
                     WEHNVRAVFEQYGISQKADVEQIVEQILRLSQKQTVRSQQSEVLTSSVRPIPQLDSPV
                     TDHDSWITQAMINTQKLPKIFYQGANNILQAIK"
     gene            2445..3347
                     /locus_tag="PLJ02_00655"
     CDS             2445..3347
                     /locus_tag="PLJ02_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04964.1"
                     /translation="MATIETNHNFKEDSEAEDQQHKAPDTSGSSITHWSVEAGEVKDG
                     VEADLIKIATENLAEDAVTKVTGLRFLEENDLPRYQKVMVNLKSFLHNPQRVFAKLPS
                     ASGLYYSSIVNLTTGERVFGLDQDQEAVKKFIREKLKNEEISPDSQLILSEYWRNYYG
                     GNLVINERGKIFAELVEGKHAKLDKGEGQIFLGAKTSDYSETLQFSEKQEMSDELKTQ
                     LRRAIIKAIELIPKHLVPLSDNPTRRFKDAVVDEAGEAAVSVPVAGYFEFILTKKDPQ
                     MDNFEVIFIDARTGDAAKKYQLLE"
     gene            3425..3495
                     /locus_tag="PLJ02_00660"
     tRNA            3425..3495
                     /locus_tag="PLJ02_00660"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3457..3459,aa:Trp,seq:cca)
     gene            complement(3508..4542)
                     /locus_tag="PLJ02_00665"
     CDS             complement(3508..4542)
                     /locus_tag="PLJ02_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04965.1"
                     /translation="MENDQEKKSGGEKTIPKTSETPKTIEPLEISYKVDEQEININSE
                     DINQLFADIKKYNDQFSPGAMITRSIFSIFEPRTKLGGDKTQETPEEHLDEETDNQQV
                     VAFFVISKHKKLLEKLGLSLKPVAEGATIFVVGDDREAKQVQMMIDVVNKDHFLAFLN
                     ELTPEQVESAGLKQNLEKIISTLSQQIVGHYDLVAPEQEAMELFAGMEKIIAEYKRLK
                     INGPALDELEKYLQHGKNGSLREYVAIKQKGLLSPPGESFGPADWQKDSTPAKLEQSW
                     NEAISVLKNTKENPQAIRLYEELSEHLKMCVEMAIKSLESLKYWDAATIDKLRTILET
                     AQQKINELAM"
     gene            4708..5130
                     /locus_tag="PLJ02_00670"
     CDS             4708..5130
                     /locus_tag="PLJ02_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017762468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOI04966.1"
                     /translation="MKNLIFNSLIPELSVSNFKESLDFYCDILGFKIEYQRQESNFAF
                     ISLEGSQLMIEEINNHWSVEKMEKPFGRGINFQIEVKKIKPILDSLAKHNYPLFKDVM
                     ENWYRQDNKLLGNKEFLVQDPDGYLLRFSEDLGEKELK"
     gene            5235..5849
                     /locus_tag="PLJ02_00675"
     CDS             5235..5849
                     /locus_tag="PLJ02_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04967.1"
                     /translation="MSNNNQHSLSDLIEKIEQLLLKQKVVVVALDGRSGAGKSTISSV
                     LLAKFDAVLVCCDDFYAGAEDGDESNWYKKTVVEKFNQVMDYQRLRSEVLNNLLVGRD
                     ASYHPFDFKRGKGLSKKSILLKVAPLVIVDGIYSGKKLRDLLDLAVLVELADKQRRKR
                     LIDREGAKFMKDWHAKWDKVEDYYFEEVMPKNQFDFVIEGIVEQ"
     gene            complement(5884..6315)
                     /locus_tag="PLJ02_00680"
     CDS             complement(5884..6315)
                     /locus_tag="PLJ02_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04968.1"
                     /translation="MPKSTEVQRHYLKITDQLVKSAIAHPRSFKKINQLNPIQIEAIE
                     SGIQDVLTIIVKIQNLTVNLNSEEKNLCLDVVTTLIENKNSDFPLAWQVFFGENPERG
                     EELYSIIEEMRDLMMQLVENHIDINEYAVIINDIAKSRFGA"
     gene            6477..6701
                     /locus_tag="PLJ02_00685"
     CDS             6477..6701
                     /locus_tag="PLJ02_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04969.1"
                     /translation="MQAELFKLQQVLANFLIDQLENKEISIKRAAEIAKQALSIIPDE
                     ENPNIKLDEIEQQLITIPELSKLDFKLILK"
     gene            6707..11320
                     /locus_tag="PLJ02_00690"
     CDS             6707..11320
                     /locus_tag="PLJ02_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04970.1"
                     /translation="MKDTGGIEIIEEKPAKKDAVVNNVVIDKDEPPTKPMVPEADHLP
                     ENQADKVENSAENSAENPEQFSRLKRWFGILRQVCQEKPRLVLKSLQNPKTLIEKSPD
                     DALAVQAEKFRWTTIDQERARIKFEKKGIDPDKAIGVINFLRNHAQQSYYPRDKNGLP
                     DIDRRFQANYQYDFNKDFKYISELVSLNDDPVGLLENIKNIVVLDEFFSLKDLVDYTK
                     SVAGNPRANEILSQIKLLKNTKKINVAYNLKLVEGENGKVEVYLGSGFGYEPFIVEPL
                     QSFAKIDEKTYQELTSQENMLRVQELWTRLAGEQPLELQFLPGLVKLAHNPQLELLFL
                     KTLEANGGYVGSNERRFGEQFLWYSHADRFDWTISRLEQMLQAVPEIFDLLDNGFVLH
                     RDILRINYENFERATDISELIERKEELVAAANDSELMLRASILKKHGLQMPMVNVSQD
                     YLNFVRTSDPDRLEKALTIYEVAKGVFNTDLVDNYYSDMHKRFDIWRKIGDEGANFSV
                     TDIAEIFELANKHKDVLANYNLLDCGLLDNLDSIDTLRQRVQALINPETQTILRDNIF
                     NDFLKLHSDYEMSSTRLPALMKNPEAYLALYAKKDLFYQRVQALINEKVNVFGDLFSF
                     YRLVQFTEDEFNQLKKDLSSAIDYKKIPFKYSTLQKFDLLSNFNEDEKENLLKNLKSL
                     GVESSTQSLYELNLDGLEKYISLSDAERQQVFDLAQKLFADFNLLITDRTDSNKLYLD
                     FYADLINNPQDQQLFFQYLNLHQQYFLQFLNNLDKSSHNKSDISKYMHLYANYFRQLL
                     NNHQGEADFVISEYIDQEGVIKEKFLQELLITTHPSEYAKISILLSDQSLAKMDEKDQ
                     RFWTYWRQSRSYLCAFLLEHRADFDRLVVNGVATEDLIIELLSIKQNDLRHNLIDSLL
                     EDPQVLANMSEDSRQFWTYWKNKKFPLQDFLIKRREDFKKFVIDGVATETLLIELFKL
                     EQNNTFMMNEVLRDPQVLANMSEDSRQFWAFWQNGPPYLEKVLMNNRERFNEFVKDNQ
                     PTYQLIAFLAKEEPAEFLGDKHSGLTDISHFAQHEKTIILTNLLEGLTKNQHLISTHF
                     NVIHQELSAHLKENGHLSEALQAPFQQAFGFALTEDFIRKSDSFYQDLHAEQYAALIT
                     DPEAYWVSNSPLLTVFEQVGWPMKDFDRFRSLVQNPVTNDILRTTDRQLLIDLSKNIG
                     LNPNNIFYMNKNSLNWLRSGLNDEKRKAFFKKILNEKPSSFNSCVDAFNLIGSENVDQ
                     VVSEPLYTERLLEAIEEFGNITPSLVTAYILNPNEHAQEEYREKVHNFRRDVHHNVPI
                     KPLAEGIGGIDFLADMIAMTFPGTNYEQIKKDLVVLEDRCADIADLKIREKGYQGKIV
                     SGEKIAQLKDENKPIEEGVINLIKVIFADQQEETYLKQGGDVNLAFQGWARLLVEAGA
                     TNQEVLFKDNLPEVIACSRVSLGDKLAMFAEKIAGDTSQLSAKNDVLNKAKELFGIYY
                     KDNASEAITLFLEQHQEQARTLLGRLSEKKLNALEKNIDLNPT"
BASE COUNT         3722 a         1867 c         2239 g         3788 t
ORIGIN      
        1 cttcaatgta cggtgtgtaa ggcttttggt tacgttagca gctataataa aaataacgaa
       61 ttactcaaaa agcaaactag cggtgagaac actttccctc tcaataaata ctgcaaaaaa
      121 tgtcaaaaga gaactcctca tcgtttgatg aaaaaactca agtaatcaat tgttccaact
      181 gtggtttagt agtcctgttt attaaagact tttagtaagt ttttattttt accgtcttca
      241 aagaaaaaat gcttcagcag acctaggtct ttgaggtgat taggattgtg tttgtcatag
      301 tgaatagaga aaatgtcttg acctttgatg cgatcataag ctaagtgaat gtggccaagg
      361 cgcaaagatt ttttgtgatt gccactagta acaatcaatc ttgataaaat ttgtcgaggt
      421 ttaagatttt tcaataattt tttgttttgt tgataaattt gagcaaatgg accagtattg
      481 agccaattgc caatctccac attaaatacc cccctagggt atttctgacg caagtaaatt
      541 gaaagcaaga gtgatctttg cgtttgaaaa tcagctagtt gccaaaactt ttgaaagaag
      601 acttcttttc tcaattttat taattcttgt tgtagagctt gctcgagaaa ttgattgcag
      661 tcggccaccc tgtctagagg caaatccaga ggtataaccc ctttccattt ctttttagga
      721 aattggcgac aagtaattaa ttggtagctc tgttgttgct cgtcaaaaca acagtaatag
      781 cgagttaaag tcataactat ttgatagctt ggagaatatt atttgcccct tgataaaaga
      841 ttttgggtag tttttgggtg ttgatcattg cttgagtaat ccaactgtca tgatcggtaa
      901 caggactatc gagttgggga atgggacgaa cactgctagt aagaacttcg ctttgttgac
      961 tcctaacagt ctgtttttga ctgaggcgaa gaatttgctc gacaatctgc tccacatcag
     1021 ctttttggct aatgccatat tgttcaaaaa cagcgcgcac attatgttcc cattcacccc
     1081 attcttttag cgttaatagg gctgatccag agagggcggc gtcgtaggcc atatctccag
     1141 acggtttact aataaacaca tcagcccaag ggaaaccata acgaatcagc aactcattgg
     1201 catcgatgat ctggggatga taaataatgt gaaatttaat ctgactttgt ggctgacggt
     1261 gggcgcggtt taaccagccg gcttgggtaa aatcagcggg gtcgtgagga ctgattaaat
     1321 ggtatttaat atgatgtttt ttagctaatg tcactgccat ttgttggtga tcagcatgag
     1381 tgccagcgta atacataatt tcaatttttg gcaagtctgt tgcgacatgg ttgagtaacg
     1441 gaagaagttg atcgaggatt tttttaatct cttgcttatt ggtaccgagt ccgccggtag
     1501 taatgagaat tttaagaggt cgctcattgg tccagggttt ttttgactga gcggcggcga
     1561 gcgtgcgggg atcaactggg gggccggtaa caatcacttt gtcttggatt tgttcggcag
     1621 ggatcttgat ctgctccgcc gccgccactg agaaaaaatc ctctttagtt ttttcatcaa
     1681 aaaccataaa gcggatatta gcttgatcag cattttgaac gtactcactt ctgacgtgag
     1741 gatcagtgat catttgtaaa acttgcttac catttgcggc tagaatattg ccagtagcga
     1801 tatgaaaaga gagaattggc cgatctagat tttgggttaa aaatttggtt gctggttcaa
     1861 tattttctag aattggtaat tttgaccgac caaagcgatc aaccacatct tttagaatct
     1921 tgagcaacgg ttgtcgggcg acagttcgac caatggtgtc agtcaatatg acttgtttcc
     1981 agtcactttt tttaatgtgg ttagtaggat ctaccaacca ggctttttta gcatcaattt
     2041 tgttacccca agcagcaact gccaccgcta atgccattga gtagtgtgct cgagaaaaaa
     2101 cgatgtcttg aatttgatca ttttctggtt caccatgaaa acctttaaga tcttctttga
     2161 atgaagcgga aagggtgata attggcacct tagtattttc tagtaatttt ttgtcttggt
     2221 agagaagagc aaaggcttgt tcctgcatat gattagccta gcagtttttt ggaggctgcg
     2281 ctagagtggt tttttagtgc taattgttaa tcaatgagaa gtttgaatgt agcatttggg
     2341 tgcaggtttt gggtttttta tagcccttta tgataaaata cctacaaggc ttaactttta
     2401 ttaaatttct taagtcatta ttaaaaagta attattaaat ctacatggcg acaatagaaa
     2461 ccaatcacaa ttttaaagaa gattcggaag ctgaagatca gcaacataaa gctccggata
     2521 ctagtgggag ttccattact cattggtcag tagaagccgg agaagtaaaa gatggagtcg
     2581 aagctgatct aatcaaaatt gctaccgaaa atttagctga agatgcagta actaaagtga
     2641 ccggcttacg ttttttggaa gaaaacgatc taccaagata ccaaaaagta atggtgaatt
     2701 taaaaagctt tttacacaat ccacagcgcg tttttgccaa attgcccagt gcaagtggcc
     2761 tttattacag ctcaattgtc aatctgacga ctggagaaag agtattcggt ttagaccaag
     2821 accaagaagc agtgaaaaaa tttattagag aaaaattaaa aaatgaagaa attagccctg
     2881 attcgcaatt gattttatct gaatattggc gcaattatta tggaggcaat ttagtaatca
     2941 atgagcgggg caaaattttt gctgaattag ttgaaggcaa gcatgctaag ttggataaag
     3001 gtgaaggaca gatttttctg ggtgcgaaaa ctagtgatta cagtgaaact ttacagttta
     3061 gcgaaaagca agaaatgagc gatgaactta aaacgcaatt gcgccgagcc attattaaag
     3121 ccattgaatt aatacctaaa cacttagtgc ccctcagcga taaccctaca agaagattta
     3181 aagatgcagt ggttgatgaa gctggtgaag ccgctgtatc tgtgcccgta gctggttatt
     3241 ttgaattcat tctaactaaa aaagacccac agatggataa ttttgaagtt atttttatcg
     3301 atgcccggac tggtgatgca gctaagaaat atcaattgtt ggagtagtga aatagtaagc
     3361 agtttggcgc aaaatatttt cactaaagta cctaattaac tgctataata actgttatta
     3421 acaaggggcg gtagttcaac ggtagaatcg aggtctccaa aacctttgat gagggttcga
     3481 ttccttcccg ccctgcaaaa cttgtagcta cattgctaat tcgtttattt tttgttgggc
     3541 agtttccaaa atcgttctca gtttgtcaat ggttgccgca tcccaatatt ttaaactctc
     3601 caggcttttt atagccattt caacacacat tttcaagtgt tctgatagtt cttcatacaa
     3661 tctaatagct tgcggatttt ctttagtatt ttttaaaact gaaatagcct cgttccaact
     3721 ttgttccaat tttgcaggag tagaatcttt ttgccaatct gcaggaccaa aagattcacc
     3781 tggcggactc agaagacctt tttgtttaat cgccacatat tctctcaaac tgccgttttt
     3841 gccatgttga agatacttct ccaattcatc taatgcaggt ccattaattt ttagtcgctt
     3901 atattctgca attatttttt ccatccctgc aaatagctcc atagcttctt gttctggggc
     3961 aaccaaatcg taatgtccaa cgatttgttg agagagagtt gaaattatct tttccagatt
     4021 ctgtttaagc ccagctgatt ctacttgttc tggtgttaat tcatttagga aagcaagaaa
     4081 gtgatcttta tttacgacgt caatcatcat ttgcacttgc ttggcttctc tgtcgtcacc
     4141 gacgacaaaa atggttgctc cttctgcaac tggtttaaga gataacccca gtttttctag
     4201 taattttttg tgtttagaaa ttacaaagaa agcaactact tgttgattat cggtttcttc
     4261 atctaaatgt tcttctggcg tttcttgagt tttatctccc cctaatttgg tcctgggttc
     4321 aaaaatacta aaaattgatc ttgttatcat ggcgccaggt gagaattgat cattatattt
     4381 tttaatgtca gcaaatagct gattaatatc ttctgaattg atatttattt cttgctcgtc
     4441 cactttatag cttatttcaa gtggttctat agtttttggg gtttctgagg tttttggaat
     4501 ggttttttct ccacctgatt ttttttcttg atcgttttcc attgactgtt ctttcttttt
     4561 ttaaaactaa tattcactca agattgattg taacaaatat gattgaatca aaataggtac
     4621 taatattttc caccaggccc tgctcaatat attttgatgt atataaatag aaacttatac
     4681 ttatttcttc ctgtataata tctataaatg aaaaatttaa tatttaattc actaattccg
     4741 gagttatctg tttctaactt taaagaaagt ttagattttt attgtgatat tttgggtttc
     4801 aaaattgagt atcaacgcca agaatcaaat tttgctttta tttctttaga aggttcccaa
     4861 ttaatgattg aagaaattaa caatcattgg agtgttgaaa aaatggagaa gccatttggc
     4921 agaggtatta attttcaaat tgaagttaaa aaaattaaac caattttaga ctctttggct
     4981 aaacataatt atcctctttt taaagatgtg atggagaatt ggtatcgcca ggataataag
     5041 cttttgggaa ataaagaatt tttggttcaa gatccagatg gttatttgtt aagattcagt
     5101 gaagatttgg gagaaaaaga attaaaatag gtttcgacct ttttcaccaa cccctcgtaa
     5161 atttttcaaa atgtcgctat aaatcagtca gtatttaaaa tatagtgaat ttaatatgtt
     5221 atataatggt tggtgtgtct aacaacaacc aacattcatt atctgatctg attgagaaga
     5281 ttgaacaact attactcaaa caaaaagtag tagttgttgc tctagatggg agaagtgggg
     5341 ctggcaaatc tacaatctct tcggttttac tagcaaaatt tgatgcagtg cttgtttgtt
     5401 gtgatgattt ttatgctggt gcggaagatg gcgatgaaag caattggtat aagaaaacgg
     5461 tggtagaaaa atttaatcag gtgatggatt atcagagatt aagaagtgag gtgttaaata
     5521 atttactagt gggtcgagac gctagctatc atccttttga tttcaaaagg ggaaaaggct
     5581 tgtcaaaaaa aagtattctg ctcaaagtcg ctcccttagt tattgttgat ggtatttatt
     5641 cgggcaagaa acttcgagat ttgctagatc tggctgtgtt ggtggaattg gctgataaac
     5701 agcgtagaaa aaggttgatt gacagagaag gggcaaagtt tatgaaagat tggcacgcca
     5761 aatgggataa agtggaggat tattattttg aagaagtgat gcccaaaaat caatttgatt
     5821 ttgtgattga gggaattgtt gaacaataat ttttatctat ataagacaca ttgaagagtg
     5881 attttaggca ccaaatctac tcttagcgat gtcattaata attactgcgt attcgtttat
     5941 atcaatatga ttttccacta attgcatcat caaatctctc atttcttcaa taattgagta
     6001 taattcctca cctctttctg gattttcgcc aaaaaagact tgccaggcta atggaaaatc
     6061 actatttttg ttttctatca aagtagtgac tacatccaaa cagagatttt tttcctcact
     6121 atttaagtta acggttaaat tttgtatttt gacaataatt gtcaaaacgt cttgaatacc
     6181 agattcaatt gcctctattt gaattggatt aagctgattt atctttttaa aagaacgagg
     6241 atgtgcaatt gcagatttta ccagctgatc tgttattttt agataatgtc tttgaacttc
     6301 ggtacttttt ggcataattt tttttaatct taaattgctt ggtctgctat tataacaaaa
     6361 atggggtaac tctcgtcagt atccaactga cttctttcac taatccttag agttgccact
     6421 ttgtattcac ccaaaaccaa gttgtattca atttttttcc tctctataat gatgctgtgc
     6481 aagcagaact ttttaagctc cagcaagtac tggctaattt tttgattgat caattggaaa
     6541 ataaagaaat ttcaattaaa cgcgctgccg aaattgccaa gcaagcttta agtattatcc
     6601 cagacgaaga aaatccgaac attaaattag atgaaattga gcaacaatta ataactatcc
     6661 ctgaattatc taaattagat ttcaagttaa ttttgaaata atagcgatga aagatactgg
     6721 aggaattgaa ataattgagg agaagccagc taaaaaagat gctgtagtta ataatgttgt
     6781 aatcgataaa gacgagccac caactaaacc aatggtgcct gaggccgatc acttaccaga
     6841 aaaccaagcg gacaaagtag aaaattcagc agaaaattca gcagaaaatc cagagcaatt
     6901 ttccagacta aagcgctggt tcggtatttt gcggcaagtt tgtcaggaaa agcctaggtt
     6961 agtactaaaa agtttacaga atccaaaaac tttaattgag aaatctcccg atgacgcgtt
     7021 agctgttcag gcagaaaaat ttcgttggac aaccattgat caagagcgag ccaggattaa
     7081 atttgaaaaa aaaggtattg atccagacaa agctattgga gtaattaact ttttaaggaa
     7141 tcatgctcaa caaagttatt atcctagaga caaaaatggt ttgccggata ttgatcggcg
     7201 ttttcaggct aattatcaat atgattttaa taaagatttt aaatatatta gtgaattggt
     7261 aagtttgaat gatgatccgg ttggtttatt ggaaaatatt aaaaatattg tggtattaga
     7321 tgaatttttc tccttaaaag atctagttga ctacaccaag tcagtagctg gtaatcctcg
     7381 ggctaatgaa attcttagtc aaattaagtt gttaaaaaat actaaaaaaa tcaacgtcgc
     7441 ttataattta aagttggttg agggagaaaa tggcaaagtt gaagtctatt taggatcagg
     7501 atttggttac gagccattta tagttgagcc cctacaatct ttcgccaaaa ttgatgaaaa
     7561 aacttatcaa gaattaactt ctcaagaaaa tatgctgagg gttcaagaat tatggactcg
     7621 tcttgctggt gaacaaccgc ttgagctgca atttttacca ggtcttgtaa aattagccca
     7681 caatccacaa ttagaattgc tttttctgaa aacattggag gctaatggtg gttatgttgg
     7741 ctctaatgaa cgacgtttcg gcgaacaatt tttgtggtat tctcacgcgg atcgattcga
     7801 ttggacgatc agtagacttg aacaaatgct tcaagctgtt ccagagatat ttgatttgct
     7861 tgataacggt tttgtcttgc accgcgatat tttgcgtatt aattatgaaa actttgagcg
     7921 ggccactgat attagtgaac taattgaacg caaagaagaa ttagtcgctg ctgctaatga
     7981 ttcagagtta atgctcagag ccagtatcct aaaaaaacat ggtttacaaa tgccgatggt
     8041 gaatgtgagt caagattatt tgaattttgt gcgaacttct gatccagatc gtttagaaaa
     8101 agccctgact atctatgaag ttgccaaagg ggtttttaat accgatttag tagacaacta
     8161 ttatagcgat atgcacaagc ggtttgacat ctggcggaaa atcggagacg agggggctaa
     8221 tttttcagta accgacatcg ccgaaatttt tgagttagcg aataagcata aagacgtgtt
     8281 ggcaaattat aatctactgg actgtggact tttagataat cttgattcaa ttgacactct
     8341 tcgccaaagg gtgcaggctc tcatcaatcc agaaactcaa acaattcttc gtgataatat
     8401 ttttaatgat tttttaaaac ttcattctga ttatgaaatg agtagcactc gtttaccggc
     8461 cttgatgaaa aatcccgaag cttatttggc tctttatgca aaaaaagatt tgttttatca
     8521 aagagttcaa gcattaatca atgaaaaagt gaacgttttt ggtgatctgt tttcctttta
     8581 tcgcttggtg caatttactg aggatgaatt taatcaactg aaaaaggact tatcaagtgc
     8641 tattgactat aaaaaaatac catttaaata ctctacctta caaaaatttg atttgttaag
     8701 taattttaac gaagatgaga aagaaaattt attaaaaaat ttaaaatcac taggtgttga
     8761 aagtagtact caaagtcttt acgaacttaa tcttgatggt ttggaaaagt atattagttt
     8821 aagtgacgcc gagcgacagc aagtatttga tctagcacaa aaattatttg ctgattttaa
     8881 tttgttaatt accgatcgga cagacagcaa taaactttat ttagattttt atgctgattt
     8941 aattaataat ccacaagatc agcaattgtt ttttcaatat cttaacctgc atcaacaata
     9001 ttttttgcaa ttccttaata atttagataa atcttcacat aacaaaagcg atatttctaa
     9061 atatatgcat ctttatgcta attattttag gcaattgctt aataatcatc agggtgaagc
     9121 tgattttgtg attagtgaat atatcgatca agaaggagtc attaaggaga aatttttaca
     9181 agagctatta atcactactc acccttctga atatgcaaaa atttctattt tgttatcaga
     9241 tcaatcttta gccaagatgg atgaaaaaga tcaaagattc tggacttact ggcgccaatc
     9301 gaggagttat ttgtgtgctt ttttgcttga acatcgagct gattttgacc gtctggtagt
     9361 aaacggagtg gccacggaag atttaatcat tgagttgctt agcattaagc aaaacgatct
     9421 aagacataat ttaattgatt cattgttgga agatccacaa gttttagcca acatgagtga
     9481 agacagtcgg caattttgga cttattggaa aaataagaag tttcctttac aagatttttt
     9541 aattaaacgt cgtgaggatt tcaaaaaatt tgttattgat ggagttgcca ccgaaactct
     9601 tttaattgaa ctatttaagc ttgaacaaaa taatactttt atgatgaacg aggtattaag
     9661 agatccacaa gttttagcca acatgagtga agacagtcgc caattttggg cgttttggca
     9721 aaatggtcca ccttatttag aaaaagtact aatgaataat agagagcgct tcaatgaatt
     9781 tgttaaagat aatcaaccaa cttatcagct tattgctttt ttagctaaag aagagccagc
     9841 tgaattctta ggagataaac attctggatt aaccgatatt agccacttcg ctcaacatga
     9901 aaaaaccata attctaacta acttgttaga aggcttgact aaaaatcaac atttaattag
     9961 tacccatttc aatgtaattc atcaagagct cagcgctcat ttaaaggaaa atggtcacct
    10021 gagtgaagcc ttacaagcgc cgtttcaaca agcttttggt tttgctttaa ctgaggattt
    10081 tattagaaaa tcggatagtt tttatcaaga tttacacgcc gaacaatacg ctgctttaat
    10141 tactgatcct gaagcttatt gggtgagcaa tagtccttta ctcactgtat tcgaacaagt
    10201 tggttggcca atgaaagatt ttgatagatt tcgtagttta gtgcaaaatc ctgtcactaa
    10261 cgatatttta aggacgactg ataggcaatt attaattgat ttatctaaaa atattggtct
    10321 gaatcctaat aacatttttt acatgaataa aaattcgcta aattggttac gatctggcct
    10381 gaatgatgaa aagcgaaaag cttttttcaa aaaaatcctg aacgaaaaac cttctagttt
    10441 caacagttgt gttgatgcat ttaatttaat aggttcagaa aacgttgatc aagtggttag
    10501 cgaaccccta tataccgaaa gactattgga ggctatagaa gaatttggca acattactcc
    10561 ttctttagtg acagcttaca ttttaaatcc aaatgagcat gctcaagaag agtatcgcga
    10621 aaaagttcat aatttccgcc gagatgttca ccataatgtt ccaattaaac ctttggccga
    10681 aggtattggt ggtatagatt ttttagccga catgatagcc atgacttttc ctggaaccaa
    10741 ttacgagcaa attaaaaaag atttagtagt tttggaggat cgatgcgctg acattgcaga
    10801 tttaaagata cgcgagaagg gttatcaggg gaaaattgtg tcgggagaga agattgcgca
    10861 gttaaaagat gagaacaaac caatagaaga aggagtaatt aacctaatca aagtgatttt
    10921 tgctgatcaa caagaagaga cttatttaaa acaaggtggc gatgtcaacc tggcttttca
    10981 aggatgggct agattattag ttgaggcagg cgccactaat caagaggtgt tatttaaaga
    11041 taatttacca gaagtaatag cttgtagccg tgtttctttg ggagacaagc tagcgatgtt
    11101 tgccgagaag attgctggtg atacatcaca attatctgct aaaaatgatg tgttaaacaa
    11161 agcaaaggaa ttatttggca tttattataa agacaacgct tccgaggcga ttactttatt
    11221 cctcgaacaa catcaagagc aagcgagaac tttactaggt agattgagtg aaaaaaaact
    11281 aaatgcatta gaaaaaaata ttgatcttaa tcctacctaa aaacttaacc gcccccaata
    11341 ccctagtcgc taaccaatgg tttttaatct aaaaaccatt tttttcaatt taaccacagg
    11401 tcccaaagca ttttagatat tatcttctcc caaatagctt ttacctcatt gaggttagaa
    11461 ttttcgtcat aagaaaatcc agcgcatttt taaataaaat tgtaaatttg tgtaacaata
    11521 atcttatgag tatatcaaaa ttaaaaaaaa ggagaatcaa ttatgacgaa acgatctcaa
    11581 atttacaaat gtgaaatctg cggcaatata gtcgag
//
LOCUS       DAOSEW010000010        11418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_4784_length_11418_cov_4.004136, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000010 DAOSEW010000000
VERSION     DAOSEW010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 11418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11418
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4784_length_11418_cov_4.004136"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..1388)
                     /locus_tag="PLJ02_00695"
     CDS             complement(204..1388)
                     /locus_tag="PLJ02_00695"
                     /inference="COORDINATES: protein motif:HMM:NF019404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM43 family protein"
                     /protein_id="HOI04971.1"
                     /translation="MTEQELSPKTTTVDQRSWFQRLGTSLRGVLTGLLLFIIAFPILI
                     WNEGQAIQTHRDLEAGASIVENSDSTVIDPAQDGKLIYFTGETRTPSILTDSTFPISE
                     NAIKLKRIVEVYQWSEITQTETVEKNGGGTETTTTYSYQKVWDDQLIDSTSFQEQANY
                     RNPQSKPLENEEQVANPVSVGAYIIPDRLLSSLSDYERLNLSSDLLTTLPNAQQEQWQ
                     VFNNYIFRSADPSNPEIGDARIWYQIIRPGTVSVVAQQNGDNLEPYRTKDGKSIEMIR
                     TGAVPAEEMSEGIIQVNPFSTWLFRILAVIFMYIGLNTLLVPISTLLHVIPLFAKVFL
                     FANKLLTLVITLVLSLITIGIMWLIYQPLIGLAILLVAVAVVFLFSKLRKKQHQQLEK
                     KS"
     gene            1436..3172
                     /locus_tag="PLJ02_00700"
     CDS             1436..3172
                     /locus_tag="PLJ02_00700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOI04972.1"
                     /translation="MNKVKNSTVLILVLLIASFFRLIGLNWDQGQFFHPDERFLNMTI
                     QELQLPKNLLEYFDPQQSTLNPRHIGRNFYVYGNFPITFSKLTNEFLNNKSLTAITIT
                     GRVLSAAADLMVVLLIYLTVFLLEKNLKDKRLAIDPRSKFWAALIYALMVLPIQQSHF
                     FTTDTFLNLFVFASFYFSLKYFFEKKDLYIISAGLFFGLALASKITAISILPLNMSLL
                     ILPDVWPLKKQLAKKKIIKNCQKILLLVLLFVSTAYLVLRLTSPYMFQTASLLDINLN
                     REFLADLTELKSFSNDDTWYPPAIQWMNRSNFFGLKNLIVYGVGLISSLLSIWGIAII
                     IKRIFSLFKKVNVQKYLLVLALIIFWLGSFLIYYSQQFVQSIRYYLLIYPFLAIFAGI
                     ACSVLLKNKKSRLKLTIALLLSIAIWPLMFISVYIKDHSRIQASAWIYQHIPSGSIIL
                     SEHWDDPLPVSLATFSEPYQNYQLPVFDPDNQQKWTEITAALNRADYYILSSNRAWGS
                     IIRVPEKYPQMSQFYQDLLAGRSNYKLIAQFNSFPSLEYLGINFSINDSWADEAFTVY
                     DHPQVLIFQKIQ"
     gene            complement(3149..4291)
                     /locus_tag="PLJ02_00705"
     CDS             complement(3149..4291)
                     /locus_tag="PLJ02_00705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOI04973.1"
                     /translation="MKKITIVSDFYPPHWTGIAKSIFYLADSIKEEFFTTVLTVQFKQ
                     QLPVSEKKEGIIIKRFPVSFTLSRAKFSGALIFHFLRNIKHSDVILINSPSIHILPIS
                     VIAKIFQKKLLIFHQGDLILPKGMINYFIQQIFDLASLISFALADQLATYTQDYAHNS
                     RLLKKFPQKTQAFIPPLPYFAPTQPAGQVDQQLKRKLSTLKKKKTFLIGCAGRFVEEK
                     GFDVLLAAIIKLKEQRDDFFLVFAGETNISYEKTFFKNQGLVNQLKDNLIFLGLLNDI
                     QLNSFYQAMDLFILPSRSECFGLVQAEAMAKKTPVIVSDIPGARDPVVITGYGLLFEK
                     NNPTALMLAIKEMLNHLKSFDKNYKKVAQYFDQDKAQRALLNFLKN"
     gene            complement(4288..4893)
                     /locus_tag="PLJ02_00710"
     CDS             complement(4288..4893)
                     /locus_tag="PLJ02_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04974.1"
                     /translation="MNNKPVKVGFDLDGVLLYNPARVVRPIIALMKQKKVIVHREQLE
                     FYVPQPGLGQFFWELLHKSSVWLAPGFAQINELKQQGLIEPYLITGRFAHLKDDYLKW
                     KTKMNADQIFVKCLVNDGDEQPHLFKERLIRELKLDYFIEDNWDIVQYLDQSFNNSHQ
                     RKSSTKIIWLTNILDFRIDYHAKVHSLKQAISIINSEQQRS"
     gene            complement(4936..5583)
                     /locus_tag="PLJ02_00715"
     CDS             complement(4936..5583)
                     /locus_tag="PLJ02_00715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HOI04975.1"
                     /translation="MKNKINKMDVLVALYMTCLALAEIMSMKTIPLMRIFSYQLNVSV
                     GIFVFPILFAINDVIAEVKGRARAKQLVYISLIMIAIIMLYSIFVTWLPESARFADGE
                     AYNLIFARAARISLASLLAFIISGLLDVAIFHKIKVKTKGKMLWLRSNLSNFISQFLD
                     TFIFTTIAFYSFQEGLSANFSFIMSIVIPYWSLKCLMSVVETPLTYWGVKWLAKK"
     gene            complement(5654..6514)
                     /locus_tag="PLJ02_00720"
     CDS             complement(5654..6514)
                     /locus_tag="PLJ02_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04976.1"
                     /translation="MLSQIEQNQEKKDERGNQLTTSLQNKFSQYPELMEVASGSSTSR
                     CEAFINRPTGKVLLEALDFAQLQFSKDFEAFLLGNQQFNFNDRMGGAYTQISRMLAKI
                     LWSSEMQKISAIFLNQDNETPNFAELKLLAEYPNQDEAFCALQNILIQLLNKDQNDNQ
                     PSDIVASKDAISINEGACFDVATYYCQAYEYGRLYLKIINGQRFLEKTHGSHTLINLS
                     PIYFNGIQLPKGSLFTTVNNDEFAFLRLTPLMFDNRQDMVSAFGAEIIKAEDLGGDLL
                     KIINHDWYGY"
     gene            complement(6553..6629)
                     /locus_tag="PLJ02_00725"
     tRNA            complement(6553..6629)
                     /locus_tag="PLJ02_00725"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6593..6595),aa:His,seq:gtg)
     gene            complement(6659..6732)
                     /locus_tag="PLJ02_00730"
     tRNA            complement(6659..6732)
                     /locus_tag="PLJ02_00730"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6696..6698),aa:Arg,seq:tct)
     gene            6926..9052
                     /gene="uvrB"
                     /locus_tag="PLJ02_00735"
     CDS             6926..9052
                     /gene="uvrB"
                     /locus_tag="PLJ02_00735"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOI04977.1"
                     /translation="MKFELQAKYQPTGDQPQAIEQLTAGVEAGKKNQVLLGVTGSGKT
                     FTIANVIARTQRPTLVIAHNKTLAAQLYQELRDFFPKNAVSYFVSYYDYYQPEAYLPS
                     SDTYIEKEAMINDEIDKLRLATTTNLLTRSDNIVVASVSCIYNLGSPAEYGKFILEIV
                     EGELIERKTLLLQLSNLQYERTTNDLQRGSYRLRGDSIQLWPAYSDTAIRIDTLNNQI
                     TQIDEIDPISGQKIPVTAGENKPKHYVVYPAKHYLLDPQSQQQALQEIRQDTAQRVAE
                     LNHLGKTLEAYRLQQKVNYDLERIEEFGFVNGIENYSRYFDGRQAGEPPYTLIDYLIF
                     NQKKFKKDSFLTVIDESHMTIPQIKGMFFGDRSRKETLIEYGFRLPSAMDNRPLNFNE
                     FLERQQQIIYLSATPKPWEINQSQGEIVEQLIRPTGLVDPQVEVRSSHHQIEDLIREI
                     IKRVELGERTLVTTLTKKMAEALTDYLNDPQKINKLLKQKPKKLPKVAYLHSDIDTLD
                     RNDILDDLRAGTYDVLVGINLLREGLDLPEVSLIGILDADKEGFLRNDIALIQTIGRA
                     ARHLNGLAILYADQITQSMRKAIDETARRRQKQLQYNQEHHIDATSINKPIREKLLQR
                     EKDGDEWQNELNRTQKNQKQKSIYLQLNKHSKLNLMAINGQDLTPAERATLIKQLRLQ
                     MNKAAADLDFELAAIIRDKIAELKLL"
     gene            9133..>11418
                     /gene="uvrA"
                     /locus_tag="PLJ02_00740"
     CDS             9133..>11418
                     /gene="uvrA"
                     /locus_tag="PLJ02_00740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOI04978.1"
                     /translation="MTQTEITITGAREHNLKNVSLSLPKNKLIVFTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSSYARQFLGVMHKPDVDQIDGLSPAIAIDQKTTSHNPRSTVGT
                     ITEIYDYLRLLFARVGHPHCPNCGKEIATQSIDQIIEQILQDIEQRIKPNAGAPQKML
                     LLSPIVRNRKGEFEGLLQNLVKKGYSRARIDKDIYNLEEPLTLIKTNKHSIDVVIDRF
                     VLDKKQLNDEQEQRSLRSRLNQSIEDALHLSNGLVIVAWVDDPGFDFPEKPKKFSEQL
                     FSENLACTDCGISLDELEPRLFSFNAPEGACATCNGIGSILKINPQKIIAPALTLSEG
                     AVIPFARMMSNDSWFSRLVKQVVEAEHYDFRQTAWQNMDANFQQLLLYGSDKKYEVVG
                     ENRFGKVTSFETEFEGFVNNLERRYRETNSDFIRREIGQFMHKETCPTCHGDRLKKEA
                     LAVHVDGKNIAEVTQLTIAQSLTWIDFLQDPQTTILNQKEQNIAQSILKEIHSRLHFL
                     NAVGLNYLTISREAGSLAGGEAQRIRLASQIGTGLTGVLYILDEPTIGLHQRDNHQLI
                     KTLQELRDKGNTVIVVEHDRDIMQAADFIVDFGPLAGQEGGKIVFTGSYQELLQQNTL
                     TAQYLSRKKQVTRQEKKAQRQLIDPNETVANVFQRSKISFRGAKHHNLKAIDVDFPLN
                     TLTCITGVSGSGKSTLLDNVLHNHLAKYLGKSFDGKLAKIEQLIVPEQVKRLSLIDQS
                     PIGKTPRSNPATYTKTFDLIRQ"
BASE COUNT         3744 a         2189 c         1997 g         3488 t
ORIGIN      
        1 tttttagttg aaaaccaatg tcgacttaaa atccgttgtt tctcttgttc agtcatattt
       61 acctccaaaa ttaattaatc ccactactca acattccctc aatagtgaca aaaaaaccag
      121 tgcaaatgct atcactggta atttttcatt atcttgataa taacaggaaa aaaccaaaaa
      181 aacaagtaca aaatttactc atatcagctt tttttctcta gttgttggtg ctgttttttt
      241 cttaatttac taaacaaaaa caccactgct accgccacta acagaatcgc taaaccaatc
      301 aatggttgat aaatcagcca cataatacca atggtgatta atgaaagtac caaggtaatc
      361 actagagtta gcaacttgtt ggcaaaaaga aacactttgg caaatagggg aataacgtgc
      421 aacagcgtag agattggtac caaaagagta ttaagtccaa tatacataaa aattacagct
      481 aaaattctaa acaaccaagt actgaatgga ttgacttgaa taatcccttc agacatttct
      541 tctgcaggga ctgcacctgt cctaatcatt tcgatactct tgccatcttt ggttcgatag
      601 ggttcaagat tgtcaccatt ttgttgagcg actacactca ctgtacctgg tctaataatt
      661 tgataccaga tacgtgcatc gccaatttcg gggttgctag gatcagcact gcgaaaaata
      721 taattattga aaacttgcca ttgttcttgt tgagcattag gcaaagttgt taatagatca
      781 ctactcaaat tgagtcgttc ataatcggac agtgaactaa gcaaacgatc agggataata
      841 taggcgccaa cactaactgg attagccact tgctcctcat tttccagagg tttactttga
      901 ggattgcgat aattagcttg ttcttgaaaa ctggttgaat caattaattg gtcatcccac
      961 actttttgat aagaataagt agtggtggtt tcagtaccgc caccattttt ttccacagtt
     1021 tcggtttgtg taatttctga ccattgataa acttctacta tcctttttaa cttgatagca
     1081 ttttcactaa ttggaaaagt gctgtcagtt aaaatactcg gagtcctggt ttctccagta
     1141 aaatagatta acttgccatc ctgagcagga tcaattactg ttgagtcgct attttcaaca
     1201 atactagccc ctgcttctaa gtctcgatgt gtttgaatag cctggccttc attccaaatt
     1261 agaatgggaa aagcaataat aaacagcaat aaaccagtca aaacaccgcg caacgaagtt
     1321 cccaaacgct gaaaccagga gcgctgatca acggtagtgg ttttagggga aagttcttgt
     1381 tctgtcatag gtttagtgta tcacaaatct atctgaatta ttatataatt tagccgtgaa
     1441 taaagtaaaa aatagtactg ttttaatttt ggttttgctg attgctagct ttttcaggtt
     1501 gattgggctc aattgggatc aaggacaatt ttttcatcca gatgaacgct ttttaaacat
     1561 gactattcaa gagctacaat taccaaaaaa cctcttagaa tattttgatc cacaacaatc
     1621 aacccttaat cctcgtcaca ttgggcgaaa cttttatgtt tatgggaatt ttccgatcac
     1681 ttttagtaaa ttaactaatg aattcctcaa caacaaatcg ttaaccgcaa ttaccatcac
     1741 tggtagagtc ttatcggctg ctgccgactt aatggttgtt cttttaattt atttaacagt
     1801 ttttttatta gaaaaaaatc ttaaagataa aagacttgct attgatccca ggagtaagtt
     1861 ttgggctgct ctaatttatg cgctaatggt gctaccaatt cagcagtctc atttttttac
     1921 aactgatact ttcttaaatt tatttgtttt tgctagtttt tattttagtc tcaaatattt
     1981 tttcgaaaaa aaagatttat atatcattag cgctgggctt ttttttgggc tagctttggc
     2041 tagcaaaatc actgccattt caatcttacc cttaaatatg agtttattaa ttttgccaga
     2101 tgtttggcca ctcaaaaaac aattggccaa gaaaaaaatc ataaaaaatt gccaaaaaat
     2161 attgctgctt gtcttgttat ttgtaagtac tgcctatttg gtgttaagac tgacctcgcc
     2221 ctatatgttc caaactgctt ctttgttgga tatcaatctt aatcgtgagt ttctggcaga
     2281 tttaacagag ttaaaaagtt tcagcaatga tgatacctgg tacccgccag ccattcagtg
     2341 gatgaatcga tcgaatttct ttggcttaaa gaacttgatt gtgtacggtg ttggtctaat
     2401 tagttcatta ctaagtattt ggggaattgc tatcatcatc aagcggattt tttcattatt
     2461 taaaaaagtt aacgtacaga aatatttatt agtcttagct ttaattattt tttggcttgg
     2521 ctcttttctc atttattatt ctcagcaatt tgtccaatca attagatatt atttattgat
     2581 ttatccattt ttagccatct ttgctggcat tgcctgtagc gtacttttaa aaaataaaaa
     2641 aagccgatta aaactaacaa tagcactact gctatcgata gccatctggc cgctgatgtt
     2701 tatttcggta tatattaagg atcactcccg gattcaagct tctgcgtgga tttatcaaca
     2761 tattcccagt ggtagcatta ttttaagtga gcattgggat gacccactac ccgttagctt
     2821 ggccactttt agtgagccat atcaaaatta tcaattgcca gtgtttgatc ctgataatca
     2881 acaaaaatgg actgaaatca ctgccgcttt gaacagagcc gattactata ttttatcttc
     2941 taaccgagcc tgggggagta ttataagagt gcctgaaaaa tatccgcaaa tgagtcaatt
     3001 ttatcaagat ttattggcgg gtcgcagtaa ctataaatta attgcccaat ttaattcttt
     3061 tccaagttta gaatatttgg ggataaattt ttctattaat gatagctggg ccgatgaggc
     3121 tttcaccgtt tatgatcatc cacaggtctt aatttttcaa aaaattcagt aaggctcgtt
     3181 gagccttgtc ttgatcaaaa tattgcgcaa ctttcttata atttttatcg aaagatttta
     3241 agtgattaag catttcttta atggctaaca ttaaggctgt agggttattc ttttcaaaaa
     3301 gtaatccata gccagtgatt acaactggat ctctagcccc aggaatatcg gacacaatca
     3361 ctggagtttt tttggccatt gcttcggctt gtaccaaacc aaaacattct gatcgtgaag
     3421 gtaaaataaa caaatccatt gcttgataaa aggaatttaa ttggatgtcg tttaataaac
     3481 caaggaaaat taagttgtct tttagttggt ttactaatcc ttgattttta aaaaaagttt
     3541 tttcataact aatattggtt tcaccagcaa aaactaggaa aaaatcatct ctttgttctt
     3601 taagtttaat gattgccgct aataaaacat caaagccttt ctcttcaaca aatcgtccag
     3661 cgcaaccaat taaaaaagtt ttcttttttt taagcgtact taacttcctt tttaattgtt
     3721 gatcaacttg cccagctggc tgagtaggcg caaaataggg caatggcgga ataaaagctt
     3781 gagtcttttg cggaaatttt ttcagtaatc gtgaattatg ggcatagtct tgagtataag
     3841 tggctaattg atcagccaaa gcaaaactaa tcagactggc caaatcaaaa atttgctgaa
     3901 tgaaataatt aatcattccc ttaggtaaaa ttaaatcgcc ttgatgaaaa attaataatt
     3961 ttttttgaaa aattttagca attactgaga taggcaaaat gtgaatagaa ggagagttga
     4021 ttaaaataac atcactgtgt ttgatgtttc tcagaaaatg aaaaattaaa gccccagaaa
     4081 atttggctct actcagggta aaagaaaccg gaaaacgttt aataattatt ccctcttttt
     4141 tttctgaaac tggcaattgc tgcttaaatt gcacagtcaa aactgtagta aaaaattctt
     4201 ctttaatcga atcggccaga taaaaaatag atttggcaat accagtccaa tgaggaggat
     4261 agaaatcact cacaatagtg atttttttca tgatcgctgt tgctctgaat taataataga
     4321 tatcgcttgc tttaaactat gaactttggc atggtaatca atgcgaaaat ccaaaatatt
     4381 agttagccaa ataattttcg tgcttgattt gcgttgatga gaattgttga aactttgatc
     4441 caagtattga acaatgtccc aattatcttc gataaaataa tcaagtttta attctctgat
     4501 taatctttct ttgaaaagat gaggttgctc gtccccgtca ttaaccaagc atttcacaaa
     4561 aatctgatca gcattcattt tagttttcca ttttaaataa tcatctttta gatgagcaaa
     4621 acgtccagta attaaatagg gttcaattaa accctgttgc tttaattcat taatttgggc
     4681 aaaaccaggc gccagccaca cactggattt atgtaaaagt tcccaaaaaa attgacccaa
     4741 tcctggctgt ggaacataaa attctaattg ttcacgatgg acaatgactt ttttttgctt
     4801 catcagggca ataatcggcc gcacaactcg cgctgggtta taaagtaaaa caccgtccaa
     4861 atcaaaacca acttttaccg gcttattatt cataaggatt tattatatta cttgtcaatt
     4921 tcttaaacaa ttttcctatt ttttagccaa ccacttcact ccccaataag ttaacggtgt
     4981 ttctaccact gacatcaaac actttagcga ccaataggga ataacgatgc tcataatgaa
     5041 actaaagttg gcactcaaac cctcctgaaa agaataaaaa gcaatggtag taaaaataaa
     5101 agtatctaaa aattgagaaa taaaattgga taggttgcta cgcaaccaca acatcttgcc
     5161 tttcgtttta actttgattt tgtgaaaaat ggccacatct aacaacccgg agataataaa
     5221 agcaagcaaa ctggcgagag aaattctagc agcccgagca aagattaaat tataagcctc
     5281 accatcagca aatcttgcag actctggtaa ccaagtcacg aagattgaat aaagcataat
     5341 tatggctatc atgattaaac tgatatagac caattgttta gcccgagccc gacctttaac
     5401 ttcagcaatc acatcgttaa tcgcaaataa aattggaaaa acaaaaatcc ccacactcac
     5461 atttaactga taactaaaaa tcctcatcag gggaatagtc ttcatgctca taatttctgc
     5521 cagagctaga caggtcatgt ataaagcaac taaaacatcc attttattaa ttttgttttt
     5581 catatttctt tgaaattaat aatgacgcta gtttaacaca gtcgagaaat aatcagtcaa
     5641 gaaaaaatta aaattaataa ccataccaat cgtgattaat gatttttaac agatctccac
     5701 ccagatcctc agccttaatt atttcagcgc caaaggcgga gaccatatct tgacgattat
     5761 caaacatcaa aggtgttagg cgtaaaaaag caaattcatc attattaaca gtcgtaaata
     5821 aactaccttt gggcaattga atgccattga aatagattgg ggagagatta atcaaagtgt
     5881 gactaccatg agttttttct aaaaatcttt ggccattaat tatttttaag tacagccttc
     5941 cgtattcata agcctgacag tagtaagtgg cgacatcaaa acaagctcct tcattaatac
     6001 taatagcgtc tttactagcc acaatatcac tgggctgatt atcattttga tccttgttta
     6061 acaactgtat taaaatgttt tgcagcgcac aaaaagcttc atcttgattt ggatattctg
     6121 ctagcaattt tagttcggca aaattaggag tttcattgtc ttgattaaga aaaatggcac
     6181 tgattttttg catttcacta gaccacaaaa ttttagctaa cattcgacta atttgcgtgt
     6241 aagcgccacc cattcgatca ttaaaattga attgttgatt accaagcaaa aaagcctcaa
     6301 agtctttgct gaattgaagc tgagcaaagt ctagtgcttc cagtaaaact ttaccagttg
     6361 gccgattgat gaaagcttca caacgagaag tagaactacc actcgctact tccatcagct
     6421 caggatattg actaaatttg ttttgcaaac tagttgtcag ctgattgcca cgctcatctt
     6481 tcttttcttg gttttgttca atttgagaaa gcatcaataa aaattgtagc agttttcaaa
     6541 ataaaagaat attggggtgg ctgacgaggc tcgaacccgc gacctccgct ttcacagagc
     6601 ggcgctctaa ccaactgagc tacagccacc attttaaact ttgtatgata acaaagtgta
     6661 cgtcgggagg gattcggacc ctcgacctac tgcttagaag gcagtcgctc tatccagctg
     6721 agctaccgac gcgcaacagt aattacatta cttctggtta attaatatcg cattatacta
     6781 aaagccccaa tattagacaa actagaattc gcaagtaact taaccccgag gcattaaaac
     6841 ccttctcaaa tggggtataa tgaagggttg ccttactttt tgaatttaat aaaaaattgc
     6901 ttaaaaaagt gatgcttaac taaaaatgaa atttgaactt caagctaaat accaaccaac
     6961 tggtgatcaa cctcaagcta ttgagcaatt aactgctggt gttgaggcag gtaaaaaaaa
     7021 tcaagtttta ttgggtgtca ctggttcagg aaaaactttt accatcgcta atgtgatcgc
     7081 ccgcactcaa cggccaaccc tggtcattgc ccataataaa actttagctg cccaacttta
     7141 tcaagaactg cgggactttt ttcccaaaaa tgcggtctct tattttgttt cttattatga
     7201 ctattatcag cctgaagcct acttacctag cagtgacact tacatcgaaa aagaggcaat
     7261 gattaatgac gaaattgata aattgcgttt agcaactact actaatcttc taaccaggtc
     7321 agataacata gtagtggcct cagtgtcgtg tatttataat ttgggttcac cagcagaata
     7381 tggaaaattc attttagaaa ttgtcgaggg tgaactgatt gagcgtaaaa ctctgttgtt
     7441 gcaattaagc aatcttcaat acgaaagaac tactaacgat ttacaaagag gtagttatcg
     7501 tctcagaggc gatagtattc aattatggcc agcttacagc gacacagcca tccgcattga
     7561 cacgctgaat aatcaaataa cgcaaattga tgaaatcgat ccaatatctg gacaaaaaat
     7621 tcctgtgaca gctggtgaaa acaaacctaa acattatgta gtttacccag ctaaacatta
     7681 tttgctagat ccccaaagcc aacaacaggc tttgcaagaa atccgccaag acacagccca
     7741 acgagtggca gaattgaatc atttgggaaa aactttagaa gcttatcgtt tacaacaaaa
     7801 agttaattat gatttggaaa gaattgagga atttggtttt gttaacggca ttgaaaatta
     7861 ttctcgttat tttgatggac gtcaggctgg agagccccct tacactttga ttgactattt
     7921 aatttttaat cagaaaaaat ttaaaaaaga ctcatttcta accgtaattg atgaaagtca
     7981 tatgactatt cctcaaatta agggaatgtt ttttggtgat cgatcgcgta aggaaacttt
     8041 gattgaatat ggtttccgtt taccgagcgc catggataac cgaccactta atttcaacga
     8101 attcttagag cgccaacagc aaattattta tttatcggcc accccaaaac cttgggaaat
     8161 caaccaaagt caaggagaaa tcgtcgaaca attgattaga ccaactggtt tagtcgatcc
     8221 ccaagttgaa gtcaggtcaa gtcatcatca aattgaagat ttaattcgcg aaattattaa
     8281 aagagtagaa ctaggtgaaa gaactttagt caccaccctc accaaaaaaa tggctgaggc
     8341 cctgactgat tatctcaatg atccgcaaaa aattaataaa ttactcaagc aaaaaccaaa
     8401 aaaattacct aaagtggctt acctacattc agacattgac accttagatc gtaatgatat
     8461 tttggacgat ttacgtgctg ggacttatga cgtattggtg ggcattaact tattaagaga
     8521 aggtttagat cttccggaag ttagtttaat tggcattttg gatgctgaca aagagggttt
     8581 cttaagaaat gatattgccc tcattcaaac cattggtcgg gcggcgcgcc accttaatgg
     8641 tcttgctatt ttatatgctg atcaaataac tcaatcaatg cggaaagcta ttgatgaaac
     8701 ggcgcgccgc cgccaaaaac agcttcaata caatcaagaa caccacattg acgccaccag
     8761 cattaataaa ccgattagag aaaaattact gcaaagagaa aaagatggag atgaatggca
     8821 aaatgaacta aatcgaacac aaaaaaacca aaaacaaaaa tccatttatt tacaattaaa
     8881 taaacattct aaacttaacc tgatggccat caacggacaa gatttgacac ccgctgagcg
     8941 agccactctc atcaaacaac tgcgtttgca aatgaataaa gcagcggctg atttagattt
     9001 tgaactggcc gcgatcatta gagacaaaat tgctgaacta aaactgttat aatgcccact
     9061 catgctcact cgtgtccact catgcccact cattaacaac acaaaaaaca taaacaaaaa
     9121 aacaaattaa aaatgaccca aactgaaatt accattaccg gcgctcgtga acataactta
     9181 aaaaatgtta gtttgtcttt gcccaaaaac aaactaattg tttttactgg tctttctgga
     9241 tcaggtaaaa gttccttagc ttttgatacc ctttatgccg aaggtcaacg tcgctatgtg
     9301 gagtcactga gttcgtatgc tcgtcaattt ttgggagtca tgcacaaacc cgatgtggac
     9361 caaattgatg gtttatcgcc agcaattgct attgaccaaa aaaccacttc ccataaccca
     9421 cgctcaactg ttggcaccat taccgaaatt tatgactact tgcgactctt atttgccaga
     9481 gtcggccatc cccattgtcc caactgtggc aaagaaatcg ccacccaaag cattgatcaa
     9541 atcattgaac aaattttaca agacattgag cagcgcatta agcctaatgc cggcgcgcca
     9601 caaaagatgt tgttgctttc acctatagtc agaaatcgta aaggagagtt tgagggtttg
     9661 ctacaaaatc tagtaaaaaa gggttactcc cgagctagaa ttgataaaga tatctataac
     9721 ctagaagaac cgctaactct aattaaaacc aataaacaca gtattgacgt ggtgattgat
     9781 cgctttgttt tagataaaaa acaattgaat gatgagcaag aacagcgctc gcttcgctcg
     9841 cgcctcaatc aatccatcga agatgcttta cacctcagta atggtctagt tatcgtggcg
     9901 tgggttgatg atcctggttt tgattttcca gagaagccca aaaagtttag tgagcaattg
     9961 ttcagcgaaa atttggcctg taccgactgt ggtatttctt tggatgagtt agaacctcgt
    10021 ttgttttcgt ttaatgctcc tgaaggggct tgcgcaacct gtaatggcat tggttcaatt
    10081 ttaaaaatta atccccaaaa aattattgcg ccagctttga cactgagcga aggagcggtc
    10141 attccctttg ctcgaatgat gagtaacgac tcttggtttt cccgtttagt taaacaagta
    10201 gtagaggcag agcattatga tttccgacaa acagcttggc aaaacatgga cgcaaatttt
    10261 caacaattgt tgttatatgg tagtgataaa aaatatgaag tagttggaga aaatcgcttc
    10321 ggtaaagtaa ctagttttga aactgaattt gagggttttg ttaataatct ggaacgccgt
    10381 tatcgtgaaa ccaacagcga ctttattcgg cgagagattg gccaattcat gcacaaagaa
    10441 acttgtccaa cttgtcatgg cgaccggtta aaaaaagaag ccttggcggt gcacgttgat
    10501 ggaaaaaata ttgctgaagt tactcaacta actatcgcac aatcattaac ttggatcgat
    10561 tttttacaag atcctcaaac aactattctc aatcaaaaag aacagaatat tgcccaatca
    10621 atcttaaaag aaattcatag tcggttgcat tttttgaatg ctgtcggtct caattattta
    10681 accatttctc gtgaagcggg tagtctggct ggtggagaag cgcaacgaat tcgcttggct
    10741 tctcaaattg gcactggctt aactggtgtg ctttatattt tggacgagcc aaccattggt
    10801 ttacatcaac gagacaatca ccagctgatt aaaactctcc aggaactgcg agataagggc
    10861 aacactgtta ttgtggttga acatgatcgc gatattatgc aagccgctga cttcattgtt
    10921 gattttggac cgctggctgg tcaggaaggg ggcaaaatcg tttttacggg cagttatcaa
    10981 gaactacttc aacaaaacac tcttactgct caatatttgt ctcgaaaaaa acaagtcacc
    11041 cgtcaagaaa aaaaggcgca acgacaactg atagatccca acgagacggt ggccaatgtt
    11101 tttcagcgct ccaagatttc ctttcgaggg gccaaacacc acaatttaaa agcaattgat
    11161 gtagattttc cgctcaatac tttaacctgt attaccggtg tttctggctc aggcaaatca
    11221 actcttttgg acaacgtgtt gcataaccac ttagctaaat atttgggtaa gagctttgac
    11281 ggcaagctag ccaaaattga acaattaatt gttccagaac aagttaaacg cttgagttta
    11341 attgatcaaa gtccaattgg taaaacccca cgctcaaatc ccgccaccta cactaaaacc
    11401 tttgatctta ttcggcaa
//
LOCUS       DAOSEW010000011        11287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_4861_length_11287_cov_4.751068, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000011 DAOSEW010000000
VERSION     DAOSEW010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 11287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11287
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4861_length_11287_cov_4.751068"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="PLJ02_00745"
     CDS             complement(<1..393)
                     /locus_tag="PLJ02_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04979.1"
                     /translation="MPAKIKQNTSQHQLKKPKNDSLVQNSWQDTEDQAKAEKKFLELL
                     QNRDKNNSEISAEPENANDNSAKEIQKHLNSYLLAVKNTGCSAATIRNYKSDIKQFLD
                     FLGENNLDGLGSKPKLLAFAKYQREKGLK"
     gene            885..1643
                     /locus_tag="PLJ02_00750"
     CDS             885..1643
                     /locus_tag="PLJ02_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04980.1"
                     /translation="MKETTRKILLFLYPTTDVGQTQPDQRLLLSTAQLRFLLPDLSLA
                     GFKSLLFYLEKNQYLLKHLINEDHYYSLTNYGQEQLEQQFPALSIARRNWHGDWSMIV
                     FLEAIKTDQNFRYLRQLLLKQGCFALNRGIFLYPGKLNDLVLAELNGRYRQAVIGYQL
                     GECLVGDERIVIGQKIALSDSLQVYSGISKELDELINIIGPTKKLKDQSKIDFYSIYN
                     RFVSFLAEDSGLIQHYYPQVEGPLSILSKLQKLL"
     gene            2174..4948
                     /gene="leuS"
                     /locus_tag="PLJ02_00755"
     CDS             2174..4948
                     /gene="leuS"
                     /locus_tag="PLJ02_00755"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOI04981.1"
                     /translation="MPKISYDHQLVEEKWLKYWQDIALFQDTDLDLSASERAKKSYLL
                     FAFAYPSGSGLHVGHVESKTALDILARYQRMHGRRVFFPVGWDAFGLPAENYAIKTGI
                     HPSQTTKTAIDTFRRQVKRLAISYDFSNELATNHPDYYRWTQWLFLQLYNKGLAYQKE
                     GKVNWCPGCQTVLANEQVVSGLCERCENEVIQKDLKQWYFKIKDYQDELISGLDDLDW
                     PEATKQQQLNWIGRHAGINITYPVYAATTGGGEPVTELTCFTSRPDTNFGATFVVLAP
                     EHALAQQVAEDNQQVAEYLAKAEKKTELERQQEGRKKSGVFTGYYALNPLTKRRMPIW
                     VADFVLANFGTGAVVGVPAHDKRDFEFVQYVNRHQAQQPQQPQQSQQPQQPQQPQQSQ
                     QELPPLAVIRVVSGPEGNQDPITTVDQVYEGEGKLINSAFLNGLAVNEAIEKMMDYLE
                     EKGYGERVVNYQLRDWLISRQRYWGTPIPIVYDPEGNPHPVREEHLPWTLPNDVDFKP
                     TGESPLKSSQEFKERVERLYGKGWTPEYDTMDTFVDSSWYFLRYCDSRNQQLFADPQR
                     LEQWLPVDLYLIGPEHIVLHLLYARFFTKFLRDQGYLKISEPFAQMRHQGMILGPDGR
                     KMSKSKGNVINPDEIVEQYGADTLRIYEMFMGPLEADKPWDDRAVAGVARFLHKFYRL
                     AVEEIELIAQLWPVMDEAATNDQNETEQSPANEGAGVALVRRSALVQKLHQTIKKVTE
                     DVPKLKFNTAIASLMELTNVWEKASLSRRTARQPELLLTAEELLKLLAILAPLAPFMS
                     EELYGRAKYAWQNLADQTLGEIKCELAEARARRQALESLEFAPSIHLTTWPDYDSALA
                     QSEQITLAVQVNGKIRGELIVDRELLDDKTEILARAKALPALEKWLAGQQIVKEIYVP
                     GKIVSLVLAS"
     gene            5083..5739
                     /locus_tag="PLJ02_00760"
     CDS             5083..5739
                     /locus_tag="PLJ02_00760"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOI04982.1"
                     /translation="MQIKHLSQRLFWLIFRFLPASLIVIALVIAKPALESLRASAESL
                     ECYCPSCPENLLPEAGMQALGQIKVEIAGAVKKPGIYELELGQRRADLLAAAGGLDPE
                     ASLVFMAKTFNLSSPLEDGEKIYIPYGWEEQWQITTVKEQPVAEGNSNQPNLDQNSQA
                     LISINTADSKSLEALPGIGEKRASDIIANRPYGSINELLTKEVLTQTLFDEIKELISI
                     "
     gene            6017..7261
                     /locus_tag="PLJ02_00765"
     CDS             6017..7261
                     /locus_tag="PLJ02_00765"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOI04983.1"
                     /translation="MIRTNSEQEFFENKSQTKDIAKMGKIDVKFHLTNTHFFSVFTQE
                     MHYFKEIQNASASSWNRRLSNINQLVKGLLATSKSIIYSSFFSLKYRFFDLQNQALIM
                     REKLAWILEENFSTREATLGKAFVFADLSGAPESIYHSFKVIGILHLIAASSANIYLI
                     LSFFKPLLAFLEHFFGQKLVFFAKSLLIITYFLLINGHFNLVDSSPSILRAALSCLLV
                     ILANHYLNLSVKPLNLLLSIAILCLLINPFYLQSLAFQLSFLASFIILFYWPFIKKKI
                     AKSSIMFSITVQFCMWPLLIFYFANINLIAIPANIVIAPLVEWLTVGYFIFIASQLLH
                     LHFLADFVARMIYFANNIFFNILAYLETLPAKSIVIDTNKSLIITSLIAIQLLLVFWI
                     YRQQKHTNRKNKYRILAKWLRA"
     gene            7269..8249
                     /locus_tag="PLJ02_00770"
     CDS             7269..8249
                     /locus_tag="PLJ02_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOI04984.1"
                     /translation="MKRGVLALLLVYFLWELLSKFPDKSVELIVCDVGQGDAILLQQG
                     QFQLLIDTGPDEAVLSCLNRHLPFLDKKIDVLVLTHYDDDHIGGFASLTESYRISTIF
                     APLTDDKQSELFLELNRVFFDLIEQGTQLKQPILGQQIAYQDFSLDQSEPQLLLTFLT
                     PANLEYEFLQNMHFFQADPRLPETRLSAVGEENLVNFSKIESTNDRSIALLLEYGQLE
                     VFLSGDLEEIGELSIVNSGLIDQVDILKVAHHGSKTSSSELFLSQLRPEIALISVGQN
                     NQFNHPSPEVLANLHNVFSRVIRTDQSGDISIILLNDKFYIASPKNNSWQ"
     gene            8894..10267
                     /locus_tag="PLJ02_00775"
     CDS             8894..10267
                     /locus_tag="PLJ02_00775"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOI04985.1"
                     /translation="MFKHQLSQIALPKFQQKLPLLSINLPASESVTVMFLANTGSRYE
                     PVGKEGIAHFFEHLVFKGTQKYPTAKDLARTLDSVGAEFNAFTSKEYTGYYVKSAANN
                     FDLALDVLSEMLLAPLLRQEDIDREKGVIIEELNMYVDTPQYHVDNVFEQLLFANPAL
                     AHDIVGNKASIRSLTSEDFHHFLQRFYGPENLLLVVAGGLDKISKEPQVLVDKVTLAF
                     NKLNEKRGENRKLPTAKLTQTQQTELSELNKSAFSSRRFKLIKRASEQAHFVLAWPAL
                     SRHHPDRIALSVLSTILGDNMSSRLFNEVREKRGLCYYVHSTLEYYHDCGYLGAKAGV
                     DPKRLKEAIEATKAVFYNLANGQGEAGESGAISAKELLRAQNYLKGTTLLNLEESNNV
                     AQFYGFRQLLDDQILTPNEVIKQIEAVTVKDLNRLAKLIFQKEQLRFALIGNFANAKE
                     IRQWVFD"
     gene            10498..10986
                     /gene="ndk"
                     /locus_tag="PLJ02_00780"
     CDS             10498..10986
                     /gene="ndk"
                     /locus_tag="PLJ02_00780"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015232117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOI04986.1"
                     /translation="MKVTQTAVLVKPDGLQRGLIGEIISRFERKGLKLVGLKMLRMDD
                     RQLDEWYSEHREKEFFHDLKTFMSSMPIVAMVWEGIDAVPVVRLLVGTTLGREAEGGS
                     IRGDFGMSQQMNLIHASSSNEAAQREIGIVFDKDEIFHYHGVTEAVIYAVGEAEFDDE
                     ED"
     gene            complement(10999..>11287)
                     /locus_tag="PLJ02_00785"
     CDS             complement(10999..>11287)
                     /locus_tag="PLJ02_00785"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOI04987.1"
                     /translation="DKNATDSQVHELNRWYHFVGTYDGANIKIYKDGKLVGTTPATGN
                     LAVSDVTAKIGTYQGTNYNFNGQIDDVRIYNYALTQEQIKLLYNDNAAVRF"
BASE COUNT         3240 a         2011 c         2437 g         3599 t
ORIGIN      
        1 ttttaagccc ttttctctct ggtatttagc aaaagctaag agtttgggtt tgcttcccag
       61 tccatctaaa ttgttttcac ctaaaaaatc taaaaattgc ttgatatcac ttttgtaatt
      121 gcgaatggtg gcagctgagc agccggtatt tttgactgct aataggtagc tgtttagatg
      181 tttttggatc tcttttgcgc tattgtcgtt ggcgttttct ggttcagcgc tgatttcgct
      241 attatttttg tctcgatttt gcaggagctc taaaaatttt ttctcagctt tagcttggtc
      301 ttcggtgtct tgccacgagt tttgcacaag cgagtcattt tttggctttt ttagttgatg
      361 ctggctagtg ttttgcttta ttttggcagg cattaatttt tactgttatt ttacttgtct
      421 aacccagatg aattagacag ttttttggtt tcgtattttt aatctttttc tagtctagag
      481 aactagttga ggcgaagcaa gcagcagttt ttattcgggt ttttgtcgat ttaaataaat
      541 atccacacca ggggaaagta ctatatatat tgcagagtag tttcactcat tttcatgata
      601 ttcaaaatta attaaaagtg atcaaaaacg atcagggcag aaatagtgat cattagttat
      661 tcaccaaact ttgcacaaaa ttgctagttg tggatttact taaaagtgct gtattatgtg
      721 tagtaatatt taaaaatatg ccttttggct gggtcattac ttgagtaaat ttatttttta
      781 tgaaaatttt tacccaactg aatgaccttc gggtttgaaa aaatggggaa aagtcaaaat
      841 ttgagatttt tagcctactt tatattattg aatttattac aattatgaaa gaaactactc
      901 gaaaaattct attattttta tatccaacga cggatgttgg ccagactcag ccagatcaac
      961 gcttgttact aagtactgct cagttgcggt tcttattgcc ggacctgtcc ttggctggtt
     1021 ttaagtctct attgttttat ttagaaaaaa atcaatactt acttaagcac ctaattaatg
     1081 aagaccacta ttacagcttg actaattatg ggcaggagca attagaacag caatttccgg
     1141 ctctttccat tgcccgacgc aactggcatg gtgattggtc aatgatcgtc tttttggagg
     1201 cgatcaaaac tgatcaaaat ttccgctatt tgcgccagtt attacttaaa caaggttgct
     1261 ttgctctcaa tcggggcata tttttatatc caggaaaatt aaacgatcta gtgttagccg
     1321 agttgaatgg acgctatcgt caagcagtga taggttatca attgggcgag tgtttagtgg
     1381 gtgatgagcg tattgttata ggccaaaaaa ttgctttaag tgatagtttg caggtttact
     1441 ctggaattag caaagagctg gacgaactga taaacataat cggcccgaca aaaaaattaa
     1501 aagatcaatc aaaaatagat ttctattcga tatataatcg gtttgtcagt tttctagcag
     1561 aagacagtgg tttaattcag cactattatc ctcaagtaga aggccccctc agtatcctct
     1621 ctaagctgca aaaattgctc taaatatagg atttttgggt ttgttaagta gttattcaat
     1681 ttattctgtc aagtctaagc ctggccgtat agactaaaaa acgctctgta cgcgcttttt
     1741 tatccttttt ttgctatttt ttggggtgac cttttataaa gtaaggcgat tttgggtgtc
     1801 tttgtttttt aatcgaccca aaacagtttg atagattaag ttatatttat attaccgaaa
     1861 atcgattttg cgataatcga gctactagat catgcaagaa taattcgtta aaaatgcagg
     1921 aaaaagactt tgggctattg ttatattagt tcgtattata tttgcagaat ttggttaatt
     1981 ttctatgctg gttttgggtg tgttttttgt cctttactcg ttggctggga tttactgctt
     2041 cggtattagt ttgctttgct tttaataatt attgctgggc agttttgtct cattttctca
     2101 actattatag gtttttctat tgttatgctg tatgcgctct tctcaaagct tgcttttgcg
     2161 ttaaaatagc cttatgccaa aaatttccta tgatcatcag ttagttgaag aaaagtggct
     2221 aaaatattgg caagacattg ccttgtttca ggatactgat ttagacttat ccgcgagtga
     2281 acgggctaaa aaaagctatc ttttatttgc ttttgcatac cccagtggtt caggtttgca
     2341 cgtcggtcac gttgaatcaa agacggctct agatatcttg gccaggtatc aacggatgca
     2401 tggtcgacgg gtatttttcc cggttggttg ggatgctttt ggtttgccag ctgagaatta
     2461 tgctattaaa acgggcattc atccgagcca aactactaag actgcaattg acacttttcg
     2521 ccggcaagtc aagcgcttgg cgatctccta tgacttcagt aacgaactag ccaccaatca
     2581 tccagattac taccgctgga cgcaatggtt gtttttgcag ttgtataaca agggtttggc
     2641 gtatcagaag gagggcaaag ttaattggtg cccaggttgc caaacggtgt tggctaatga
     2701 gcaggtagtg agtggtttgt gtgagcgctg tgagaacgag gtcatccaga aggatctcaa
     2761 gcaatggtat tttaagatta aggattacca ggatgagttg attagtggcc tggatgattt
     2821 agattggccg gaagcaacca aacaacaaca gctgaattgg attggtcggc atgcgggtat
     2881 taatatcact tatccagtgt atgcggcgac aactggtgga ggtgagccgg ttaccgagtt
     2941 aacctgtttt actagtcgtc cagatactaa ttttggggcg acttttgtcg tcctggcgcc
     3001 cgagcatgct ttggctcagc aagtggctga agacaatcag caggtggcgg agtatttggc
     3061 taaggcagag aagaaaactg agctcgagcg ccaacaagaa ggtagaaaaa aaagtggcgt
     3121 ctttactggt tattatgcgc ttaatccgct aaccaagcgg cggatgccca tctgggtggc
     3181 tgattttgtc ttggctaatt ttgggactgg ggcagtagtt ggtgtcccag ctcatgataa
     3241 gcgggatttt gaatttgtgc agtacgttaa tcgccaccaa gctcaacagc cacagcaacc
     3301 tcagcagtca cagcaacccc aacagccaca gcaaccccaa cagtcgcaac aagaattacc
     3361 accactagca gtgattcgtg tcgtcagcgg gccagagggc aatcaagacc caatcactac
     3421 agtggatcaa gtgtacgaag gagaaggtaa gctaataaat tcagcatttt taaatggtct
     3481 agcagtcaat gaagccattg aaaaaatgat ggattatctt gaggaaaagg gctatggaga
     3541 gcgggtggtc aattatcagc tgcgggattg gttgatttct cggcaacgtt attggggaac
     3601 acccattcca attgtttatg acccagaggg gaacccgcat ccggttaggg aagagcattt
     3661 gccctggact ttgccgaatg atgttgattt taaaccaact ggcgagtcac cactcaaatc
     3721 ttctcaagag tttaaggagc gagtggaacg cttgtatggt aaaggttgga cgcccgaata
     3781 tgacacgatg gatacttttg ttgactctag ctggtatttt ttgcgctact gtgatagtcg
     3841 caatcagcaa ctgtttgccg atcctcagcg tttagagcaa tggttaccag ttgatttgta
     3901 tctcattggt ccagagcata ttgttttgca cctgctttac gcccgttttt tcactaaatt
     3961 tttgcgcgat caaggctatt taaaaattag cgagcccttt gctcaaatgc gtcatcaagg
     4021 gatgattttg gggccagatg gtcgcaaaat gagtaagagt aaaggtaatg tgattaatcc
     4081 agacgagata gtcgagcaat atggtgccga cactttgcgg atttacgaga tgtttatggg
     4141 accactggag gcggataagc cttgggacga tcgagcggtg gcgggggtgg ctcgcttttt
     4201 gcataaattt tatcgtttag cagtggagga gatcgagttg attgcccagt tatggccagt
     4261 gatggatgag gcagctacca atgatcaaaa cgaaactgag caaagtccag ccaacgaggg
     4321 ggcgggtgta gctttggtca ggcgctctgc cttagtgcaa aaattacacc aaactattaa
     4381 aaaagttact gaagatgtgc ctaaattgaa atttaatacc gcgattgcca gtcttatgga
     4441 actgactaat gtctgggaaa aagctagttt atcaagaagg acggcccgtc agcctgaatt
     4501 attattgact gccgaggaat tattaaagct gttggctatt ttggcaccac tagctccgtt
     4561 tatgagtgag gaattatatg gtcgagcgaa gtatgcttgg caaaatttgg ccgatcaaac
     4621 tcttggggag attaagtgcg agttggctga agcgcgagcg cgccggcaag cgttggagag
     4681 cctggagttt gcaccatcga ttcatttgac aacttggcct gattacgact cggcgctcgc
     4741 gcagagtgag cagataacgc tagctgtcca agtcaatggt aagatccggg gggaattgat
     4801 tgttgatcgg gagttgcttg acgataaaac ggagatttta gccagggcca aagcgttacc
     4861 agctctcgaa aagtggctag ctggtcagca aattgtgaaa gaaatttacg ttccgggaaa
     4921 gatagtcagc ctggtgcttg cctcttgaat ttgtcgatag tcagcctggt gcttgccaag
     4981 caattggttg attttggtct gggattactc caacctagat gaattttccg tattctgcta
     5041 attgtcagct gtttgtcttt tttgctttag ataatgttgt caatgcaaat aaaacattta
     5101 tctcaacgtc ttttttggct gatttttcgc tttttgccag ctagtctgat agttattgct
     5161 ctggtcatag ccaaacccgc tctagagtct ttacgcgcgt cagccgagag tttggaatgt
     5221 tattgtccaa gttgtccaga aaacctttta ccagaagcgg gtatgcaggc gttgggccag
     5281 atcaaagtag aaatcgccgg agcggtcaaa aagccaggta tttatgagct agaactaggt
     5341 cagcggcgag cagatttatt agcagcggct ggaggtttag atccagaagc ttcattggtg
     5401 tttatggcca aaacttttaa cctgagtagt ccgctagaag atggagaaaa aatctatatt
     5461 ccttatggtt gggaagaaca gtggcaaata actactgtta aagagcaacc agtggcagag
     5521 ggcaacagca accagccaaa tctggatcaa aatagtcaag ccttgatctc gattaatact
     5581 gcggatagca aaagtttaga agccttgccg ggaataggtg aaaaaagagc ctccgatatt
     5641 attgctaatc gaccttatgg cagcattaat gaattgttga caaaagaggt gctgacgcaa
     5701 actctgttcg atgagataaa ggaattaatt agcatttaac aaaaaagtta taagacacta
     5761 gccaacaaaa ataagcgcaa aaagtctaag cagagaatag caacgattgc taaaaaatgg
     5821 caattttttg gagaaaaaca tatttatgca aaaataaacg ctcaacagcg aagaactata
     5881 acattttgga tcatgagggt gtatgactta taagaaaatt aatcaaataa aaagaacgtt
     5941 tttgatattg attttgatat taataataca gcaaaatttt acaataataa aaaacacccg
     6001 aaagtggcca tttttcttga tcagaaccaa tagtgaacaa gaattttttg aaaataagtc
     6061 acaaaccaaa gatatagcga aaatgggcaa aattgacgtc aagtttcact taacaaacac
     6121 tcattttttc tctgttttta ctcaagaaat gcactatttc aaagagatac aaaatgcatc
     6181 agctagcagc tggaataggc gattgtcaaa tatcaatcag ttagtaaaag gattgttagc
     6241 aacgtctaaa agcattattt actcaagttt tttttcgtta aagtatcggt tttttgactt
     6301 acaaaatcaa gccttaatta tgcgggaaaa actcgcctgg atacttgagg aaaatttttc
     6361 cactcgagaa gctactctgg gtaaagcttt tgtctttgct gacctgtctg gtgctcccga
     6421 gagcatttat cattctttta aagttatagg gatattgcat ttaatcgcag cttctagtgc
     6481 caatatttac ttgattttgt ctttttttaa gcctttactc gcttttcttg agcatttttt
     6541 tggccaaaaa cttgtttttt ttgctaaatc tctgctaatc attacctatt ttttattgat
     6601 aaatgggcat tttaatctag ttgattctag tccgtcaatt ttacgcgctg ctttgagctg
     6661 tctgttagtt attttagcca atcattacct taatttatcc gttaaaccat taaatctatt
     6721 attgtctata gctattttgt gcctacttat caatccattt tatttacagt ccttagcctt
     6781 tcagttgagt ttcctggcta gttttattat tttgttctat tggccattta ttaaaaaaaa
     6841 gattgctaaa tcaagtatta tgtttagtat cactgtccaa ttttgtatgt ggccgctgct
     6901 gattttctac tttgccaata taaatttgat tgctatacca gccaatattg ttattgcgcc
     6961 tttagtcgag tggctaaccg ttggctattt tatttttatt gctagtcagc tcctgcatct
     7021 tcatttttta gccgattttg ttgcgagaat gatatacttt gctaacaata tattttttaa
     7081 tattttggca tatctggaaa ctttgccggc taaatcgata gtaattgata ctaataaaag
     7141 cctgataatt acatccttaa tcgctattca gttactgttg gtcttttgga tttaccgcca
     7201 gcaaaaacat actaatcgaa aaaacaaata taggatatta gcgaagtggc tgcgagccta
     7261 aaagttcaat gaaaagggga gtcttggcac ttttattagt ctatttcttg tgggagttat
     7321 tgagtaaatt ccctgataaa tcagtcgaat taatagtttg tgatgttggc cagggtgatg
     7381 ccatcttgct tcaacaaggt cagtttcagc tgctaattga caccggtcca gacgaagccg
     7441 tgctgagttg tctcaatcgg catttgcctt ttctcgataa aaagattgat gtattagtct
     7501 taacccatta tgatgatgat catattggtg gttttgccag tttaactgaa agttatcgaa
     7561 tttcgacaat ttttgctccc ctaaccgatg acaagcaaag tgagcttttt ctcgagttaa
     7621 atcgggtttt ttttgactta attgaacaag gaactcagct caaacagccg attttggggc
     7681 aacaaatcgc ttatcaggat tttagcctgg atcaaagcga gccacagctg ctgttaacct
     7741 ttctcacccc cgccaacctc gagtatgagt ttttgcaaaa tatgcatttt ttccaggcgg
     7801 accccaggct acccgaaaca agattatcgg ccgttggaga agaaaattta gttaatttta
     7861 gtaaaattga aagcacaaat gaccgatcta tcgcactttt attggaatat gggcagctag
     7921 aagtcttctt atctggagat ctcgaggaaa ttggtgagct tagcatagtt aactctggtc
     7981 tgatagacca agtggacatc ttaaaagtag cgcaccatgg gtcaaaaacc tcaagtagcg
     8041 agctattttt atcgcaatta cgacccgaaa ttgctctgat ttcggttggc caaaataatc
     8101 aatttaatca cccctcacca gaggttttgg ccaatttgca caacgttttt agccgagtga
     8161 tccgtactga ccagagtgga gacatctcaa ttattctgtt aaatgacaaa ttctatatag
     8221 cgagtccaaa aaacaattct tggcagtgat cttttcagct aattttgatc actgacaaaa
     8281 aatggtcatt gctgacaact tagcaatgcg ggcagaattg ccaaattgtc tcgcatccat
     8341 cactatgcaa taaatgcaaa aaaattgcaa gtttttgtgt tcaatttaat tttttttgaa
     8401 aaaaacccaa gtaaaaatgt atccaatttt tggctaattt taatgatttt gcgaaggata
     8461 attggagggt gtttttacag tgacgggcca ttgttttggg gactaacttt ggcaaattgt
     8521 gtagcgatat ttgtgtagca gttgctttac aattgcccag cagtcgcttt actcactgtg
     8581 ccgtttactg attttttagt aaaaaaactg ccaatttttg tcgaaaaatc agccaaatta
     8641 aagctaatct ggtaccagag tagggtaatt gtctttgatt ggcgctgaca agctcaccga
     8701 aatctgtccg aaatatgaaa agggatattg taactaattt atcaaaattg agtaaatttt
     8761 ttttctcata aaacaccagc taaatttatt aagactaaaa actgtaagag taataaatat
     8821 actcaaaaat attcggcttt tatttgccac tttttaaaac tcacttgcac aattttggtg
     8881 ttacaatagc gctatgttta aacaccaatt aagtcaaatt gctttaccca agtttcagca
     8941 aaaattgcca ttattaagta ttaatttgcc agcgagtgaa tcagtaacag tgatgttttt
     9001 ggccaatact ggttcccgct atgaaccggt gggcaaagag ggtattgctc atttttttga
     9061 acacctggtg tttaaaggta cccaaaaata tcccaccgct aaagatctgg ctcggactct
     9121 agacagtgtc ggtgccgagt ttaatgcttt taccagcaag gaatacactg gttattacgt
     9181 caagtctgca gccaataatt ttgatctagc tctcgatgtg ttgtcagaaa tgcttttggc
     9241 tccgctcttg cgccaagagg atattgatcg ggaaaagggg gtgattatcg aggaattaaa
     9301 catgtatgtc gatacgcctc agtatcacgt tgataatgtt tttgaacaat tgctttttgc
     9361 caatcccgcc ttagctcacg atattgttgg caacaaagcc agtattcgct cgctgaccag
     9421 tgaagatttt catcattttt tacaacgctt ttacggtcca gaaaatttgc tgttagtggt
     9481 tgcgggtggt ttggacaaaa ttagcaaaga gccacaagtg ttagttgaca aagtgacttt
     9541 agcttttaac aagctcaatg aaaaacgagg tgaaaatcgc aaattgccta cggctaaact
     9601 tactcaaact caacaaactg agctctcaga actgaacaaa agcgcctttt ctagtcgtcg
     9661 ttttaaactg attaagcgag cgagtgaaca agcccatttt gtgctagcgt ggcccgcgct
     9721 tagtcgtcat catcccgatc ggattgcgct ttcggttttg tccaccattt tgggcgataa
     9781 catgagttcg cgcctgttta acgaagtccg cgaaaaaaga ggtttgtgtt actacgtcca
     9841 cagtacccta gagtattatc acgattgcgg ttatttagga gctaaagctg gagttgaccc
     9901 caaacgactc aaagaagcaa ttgaggcgac aaaggccgtt ttttataact tggctaatgg
     9961 tcagggggaa gcaggagaat caggagcgat tagtgccaag gagttgctaa gggcgcaaaa
    10021 ttaccttaag gggacaactc tgctcaattt agaggagagc aataatgtag cccaatttta
    10081 cggctttaga cagcttttgg acgatcaaat tttgacgcca aacgaagtga ttaagcaaat
    10141 tgaagcggtg acagttaaag acctcaatcg cttagccaaa cttattttcc aaaaagagca
    10201 attacgattt gccctgattg gcaattttgc taatgcaaaa gaaattcgcc aatgggtttt
    10261 tgattaactt attaacattt tgatatctta attaatgttt agatcgatta aagttagtag
    10321 tcatttttgt tacccttttt tattatctat tacttaacct tttgatatct tattttatat
    10381 attgatatat ctaaaatagt ccatttttgt ctcattttgt tttttgctaa ttttaagatc
    10441 atcgccagtg gcagcaaaag gatatataat aagaaatcaa taaaagagga gggaaaaatg
    10501 aaagttacac aaacagccgt tttagttaag ccagatggct tgcaacgagg tttaattggg
    10561 gaaattatta gtcgctttga gcgcaagggt cttaagctgg ttggtctcaa gatgttgcgt
    10621 atggatgatc gtcaattaga cgagtggtat agcgagcatc gtgagaaaga attctttcac
    10681 gatctgaaga cctttatgag ttcaatgcca attgtggcca tggtttggga aggcattgac
    10741 gcagtgccag tagtgcggtt gctagtgggt actactttgg ggagagaagc tgaaggtggt
    10801 tcaattcgtg gcgattttgg catgagtcaa caaatgaatt tgattcatgc ttcatcgagt
    10861 aatgaagcag cgcagcgtga aattggtatt gtttttgata aagatgagat ttttcattat
    10921 cacggtgtca ccgaagcagt catttatgca gtgggtgagg ctgaatttga tgatgaagaa
    10981 gactaattta actattcgct agaatctaac agcagcgttg tcattgtata aaagtttgat
    11041 ttgttcctgg gtgagagcat aattataaat tcttacatca tcaatttgtc cattgaaatt
    11101 gtaattggta ccttgataag tgccaatttt ggcagttaca tcactaactg ctaaattgcc
    11161 ggtggcagga gtggtgccta ctaattttcc gtctttgtaa attttaatat ttgctccatc
    11221 ataagtaccc acaaaatgat accaacggtt gagttcatgt acttgtgaat cagtcgcatt
    11281 tttatcc
//
LOCUS       DAOSEW010000012        10650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_5228_length_10650_cov_4.717508, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000012 DAOSEW010000000
VERSION     DAOSEW010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 10650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10650
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5228_length_10650_cov_4.717508"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PLJ02_00790"
     CDS             complement(<1..532)
                     /locus_tag="PLJ02_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04988.1"
                     /translation="MPNDRAKTSLLQDVDLKKYPKLSQFLTAQALATKKDTQILSNQQ
                     ALDLLEEAIDEVEDQRSQSTAPTSLSKLKEVNQVVVDNSPDLKLDQNIDSLKPSATEV
                     VANQEPQVEQPTNTEHAVAVEAVKSSESAEMAEIGQELVEIKEDDQEQAEKLAKKEQA
                     TIDQLASQALAFPKQIK"
     gene            complement(575..1087)
                     /locus_tag="PLJ02_00795"
     CDS             complement(575..1087)
                     /locus_tag="PLJ02_00795"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOI04989.1"
                     /translation="MTKKIIIKLILLIVLLASCLYFYQTTFWRLFVKDGQVSKLVLKE
                     NYFSRSKKELLVEVVLYEASTQRGLSNRTQLATVDGQKLDGMLFIFPEERVRQFWMKD
                     MLFAIDICWFQDQRLIDCTRKATKPALNQSDENLLIYQSPTLTDMVLETMPNSLPDEV
                     LGSKLFFSFF"
     gene            complement(1110..1604)
                     /locus_tag="PLJ02_00800"
     CDS             complement(1110..1604)
                     /locus_tag="PLJ02_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04990.1"
                     /translation="MSVFNQQQKKIKKFIVLAGLILFILWWLAKAQWQIARVWPEILL
                     LTIAYSLGLFLILGDEKYLQKIYGGELDNKFLVTRSPLFLLALPILSIFMLTSTGSLA
                     GIALIMAINLVILVEIWQLAPQQQVFNQYFLPGIKKEISLAEISIIKLVALLYFFLLL
                     FLLR"
     gene            complement(1634..2830)
                     /locus_tag="PLJ02_00805"
     CDS             complement(1634..2830)
                     /locus_tag="PLJ02_00805"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOI04991.1"
                     /translation="MKWRNILIVFLFFLAGFLAYNYFEQPFLKYFPEPVIDQPTEGVE
                     ETEQNLDLPELSTREKLTQMLMLPLVLDNEQDPADNDNLALIQEYQPGFVVYFGEEID
                     FATAKKYQESLLDANNKKIVLPLLAVDHEGGRVQRLSGEGFTPLPSMRQLTNAVSSID
                     EEQDTNPLNARERRKLFNQSAKELSEVGINLVLAPMVNYSAVNNPILTDRIGNNPDKI
                     VQIAQEYIVAFSNRQIMSVLKHFPGIGGSQKDLYLYADTVELDQDDTLIFERLLDQYP
                     NIGVMSAHLRLKDKLAGQACSLSKQCLDQLFQFYPEVLLITDDLLMESASYIPGTNEK
                     KNLSTIAIEAIEAGNHVLLFGADFDYAQLNSLLDDLEAQYAESLDFQKLVNQASQKIL
                     SLKGYQ"
     gene            complement(2843..3310)
                     /locus_tag="PLJ02_00810"
     CDS             complement(2843..3310)
                     /locus_tag="PLJ02_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04992.1"
                     /translation="MSVKKRKSQKPTQSITVVKQSLFQDNSNDDETKLFGKEKTVEKQ
                     SNQRKKQFSTVAELLNQHPIKESNKYISQEFQAFGCYLAAVLGDQAHTSLYIKLAKTY
                     PRALLERALAFVSDAENARSKAKLFMWKLDQLKKKKATTVDKQKTTSEKKIKF"
     gene            complement(3343..4032)
                     /locus_tag="PLJ02_00815"
     CDS             complement(3343..4032)
                     /locus_tag="PLJ02_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04993.1"
                     /translation="MSKSNQSAEPAVFQPVPLVVVDGDEGVQLNYGRRFAFLGSGFGL
                     PHFSEVINSLQTVIDSNFPKEQLDKKLKFAASEENAWLKENLELNDWDETNASAQQHV
                     AALADRLNFEYVGILPFSDPKELKSGVRGHMVRPHNMHLANQVCFTIAGGEEVYNLGQ
                     YVISADWLAAFTNNGKKNMKEAKEILQTQLDFYQTLAGDNKLKVLIEERGLFKSKLVE
                     KNRQILAQIIL"
     gene            complement(4056..4208)
                     /locus_tag="PLJ02_00820"
     CDS             complement(4056..4208)
                     /locus_tag="PLJ02_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04994.1"
                     /translation="MAKQRMIVTDDQLLIDMELLFNAIREANGDWEQVEKEMNQYLAA
                     VQAMTQ"
     gene            complement(4323..5432)
                     /locus_tag="PLJ02_00825"
     CDS             complement(4323..5432)
                     /locus_tag="PLJ02_00825"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOI04995.1"
                     /translation="MAKSTKKMTMEELLKANADNLVVPQKGDTVTGVIVEKNKKNLVI
                     DLGAKTEAYVADKEFEFANDFIEDLKVGDEIKGIVISTDYDNGQVLLSLKGAASDAKW
                     EYFEEALDKDLDLEAKGVELNRGGLIVLVNGVRGFVPSSQFGRQYMGKLPSLKGETFK
                     VKAIEVDREKNRLIFSERHVSEAKELAQKDLAIEAVREGEIYEGVVSGVMHFGLFVTV
                     EVPVKDENGKDSTGYIEGLVHISEISWEKVNHPRDYHKVGDRVKVKVLGIDERTNKLN
                     LSIKQLEEDPWSTLAERYAAGTTFTGKVTRIEPFGVFVNVEKGVDGLIHSTKLDPNKE
                     LKVGDEVTVNVENVVPEQRRMSLSIVQTDLPIAYK"
     gene            5520..6833
                     /locus_tag="PLJ02_00830"
     CDS             5520..6833
                     /locus_tag="PLJ02_00830"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOI04996.1"
                     /translation="MINCPKIKDIKNKKILVRVDFNVPLKLVAGKAGNKNAVWRVADS
                     DRISSALETINFLLDHQAKVILISHLGRADGKKNHDLSLLPVAKFLKNNLDLPVEFVN
                     DCVGFEVAEKSKKLEAGQALLLENLRFYPGEEKNDLNFTKQLIKDSGAEVYINEAFSA
                     SHRAHASIVGLAKRLPSFAGFNLKREVEQFSKALQNIKRPLVVVMGGAKISDKVTTMK
                     NLSNLADVVLVGGGIANAMLRAGGIETHHSFIGADEEKEPAEVEKILKQAKEILSEHA
                     TERILFENVGEDRKNFPLPKIILPIDVVAGKDLDATTSEVITLLTDVADTDHDRDIKY
                     LDIGPETIRLYRYILQGAKTIVWNGPMGVFENPLFARGSREIARAIASATQHGAISLV
                     GGGESNSVINRAGLHDQFTHISTAGGAALEYLSGAELPGIKILEK"
     gene            complement(6859..>7332)
                     /locus_tag="PLJ02_00835"
     CDS             complement(6859..>7332)
                     /locus_tag="PLJ02_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04997.1"
                     /translation="QLVEKYHFFANDYFISYWRRQELAKQVFALGQQAYKAGDWSNAS
                     YFYEKSYEFDQYVFFAERALFLDESDLNNLVQFSLNFKKISPHFVGDFNRYIYLYREQ
                     LDMLFLENRLTEFRQLMINMLELEPQAKWYLLDHLFALAKNDEQRALLQEIENSY"
     assembly_gap    7333..7342
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7343..8549)
                     /locus_tag="PLJ02_00840"
     CDS             complement(<7343..8549)
                     /locus_tag="PLJ02_00840"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOI04998.1"
                     /translation="MNLLVRSYGHNHYVAFLLLILPVLWWSLFRKKTNYLFSKKIATY
                     LTIFLLISSYVIVLISLSRLGVLLVLAQFLCILLLNKQQFYNLRQKTFIYSLIKSLVF
                     VFLFASLIFIFLSLPFLDNNDCFLKTYNKDFCISLKENSRFFYWRQGYLTIKNYPLVG
                     YGLNTFKFAARRFPLPFEQQSAYAHNIFLHNLAELGLIAGGLFVFLIIYLYHQSYLKI
                     KKSKQSLNNFLFLGASSSLFNAMFDFDWHFFVIFLLTLIFLAFILKDEQRDLPIIENK
                     SNRSWLIYSLFLFLLSLIFLVGNLLTISWQKNNPKKWLKYTPFYNTAIKSAYDNTVYT
                     NTTYQSLYHLYRYDSDFIIRFLNLDEEIDQELKKQLYLDLAETYPATFVSKINFQEWS
                     TNDAKILLEK"
     gene            9129..10190
                     /locus_tag="PLJ02_00845"
     CDS             9129..10190
                     /locus_tag="PLJ02_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI04999.1"
                     /translation="MNISDLTLPFYWDTAWYLIPTSKLIHENGLLSFISYPGSDYPHT
                     FLLPSLLSLAFYLSEPQATISIHLIGILLSIFFLFVAYKLAKIFVDKKLAILMLLLVV
                     SNPIFLSQTFLVYFEIIGLALRLLTLKFFLEKKPKAFLITSLVAIFTRIDNFIFISCM
                     CLNIFFDQKQKIQEKIAFLKKYYLPSAIVTFAWFLSNKLINGWWFYSPERYFDEHHLQ
                     SFIESLRVVFIYQGRWAITVATIIGLIILILQKRWKLVFNKKLLVFFSATFPTLIMIA
                     VLGYVLSRYTLPLIIFNYYFLIIILDKIQIKNNKLGKIAFIILSLAIIQFNHKNNCFA
                     GNREDCLLVKNIIREQLIK"
     gene            10187..>10650
                     /locus_tag="PLJ02_00850"
     CDS             10187..>10650
                     /locus_tag="PLJ02_00850"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOI05000.1"
                     /translation="MTNKDLLIIKKMFTLPWPLKDKLFCLLRYITAPFNEMEKMVPKK
                     GKILDIGCGHGLFELILKSKSNKRVITAIDPDSKKIQLAKKIEELFTNLKFSDSTTNL
                     TNNNYQMDCCILSDVDYLLDGKGKTQILQQAKFNLKNTGTIIFKTVINNGSIG"
BASE COUNT         3581 a         1885 c         1664 g         3510 t
ORIGIN      
        1 gtttaatttg cttggggaaa gctagtgcct gactggctaa ttgatcaatg gtagcttgtt
       61 cttttttagc tagtttttct gcttgctctt gatcatcttc cttaatttcc acaagttctt
      121 gaccaatttc tgccatttct gctgattcac ttgatttcac tgcttcaaca gctaccgcat
      181 gttctgtgtt agttggctgt tcgacttgtg gctcttggtt ggctactact tccgtcgctg
      241 atggttttaa actgtcaata ttttgatcga gttttaaatc tggtgaattg tccacaacta
      301 cctggttgac ctcctttaat ttagaaaggc tggtgggagc agtcgactga gagcgctgat
      361 cttctacttc atcgatagcc tcttcaagca gatctagtgc ttgttgatta gacaatatct
      421 gagtgtcttt tttggttgct aaagcttggg cagtaagaaa ttgacttaat ttaggatatt
      481 tttttaaatc aacgtcttgc aatagagaag tcttagctcg atcgttaggc ataaaaccat
      541 catacaagtt tttgcttgaa gaaaaaagag agagttaaaa gaaactaaaa aatagcttag
      601 atcccaaaac ctcatcaggt aaagaattgg gcatcgtctc taacaccatg tctgtcagag
      661 ttggtgattg gtaaattaat aaattttcat cactttgatt taatgctggt ttagttgctt
      721 ttctagtaca gtcaatcagt ctttgatctt gaaaccaaca aatatcaata gcaaatagca
      781 tgtctttcat ccaaaattgg cgcactcttt cttctggaaa aataaacaac atcccatcca
      841 atttttgacc atccactgtg gctaattgag ttcgatttga taagcctcgt tgggttgaag
      901 cttcgtagag aactacctcc actagtaatt cttttttcga ccttgagaaa taattttctt
      961 ttaacactaa tttgctcacc tggccatctt tcacaaacaa tcgccaaaaa gtggtttgat
     1021 aaaaataaag acatgaggca agcagtacta ttagtagtat aagtttaata ataatttttt
     1081 ttgtcattta acgaagcaaa aattgtcatt taacgcaata aaaatagcaa taggaaaaaa
     1141 tatagcaaag ccaccaactt aattattgaa atctccgcta atgaaatctc ttttttaatt
     1201 cctggcaaaa aatattgatt aaaaacttgc tgttggggag ctaattgcca aatttcaacc
     1261 aggattacta aattaatggc cattataagt gctattccag caaggctacc ggtcgacgtc
     1321 aacataaaaa tactcaaaat aggtaatgct aataaaaata agggactacg agtaactaaa
     1381 aatttgttgt ctaattcccc accataaatt ttttgcaagt atttttcatc gcccaaaatc
     1441 aaaaatagac caagactata agcaatagtc aaaagtaaaa tctctggcca aactctggca
     1501 atttgccatt gtgcttttgc taaccaccac aaaataaata agattagccc tgccaaaaca
     1561 ataaactttt taattttttt ttgttgttga ttaaaaacac tcataaacta aaggcaatct
     1621 attaaaatat taattattga taacctttta gagataatat tttttgacta gcttgattaa
     1681 ctagtttctg aaaatctaaa gattctgcat attgggcttc taaatcatca agtagactat
     1741 ttagttgggc ataatcaaaa tccgcgccaa acaatagcac gtgattaccc gcttcaatag
     1801 cttcaatcgc aattgttgaa agatttttct tttcattagt cccaggtata tagctagctg
     1861 attccatcaa taaatcatca gttattaaca aaacttcagg ataaaattga aacagttgat
     1921 ctaaacattg tttacttaaa gaacaagctt gaccggccaa tttatccttt aggcgcaaat
     1981 gagctgacat taccccaata ttaggatatt gatctaataa tcgttcaaaa attaaagtgt
     2041 cgtcttgatc caattcaaca gtgtccgcat ataaatacaa gtctttttga ctcccaccaa
     2101 taccaggaaa atgttttaaa acagacatta tttgccgatt ggaaaaagcc acaatatatt
     2161 cttgggcaat ttgcacaatt ttatctgggt tgttaccaat cctatcggtt aaaatagggt
     2221 tattaacagc agagtaatta accattggcg ccaaaactaa gttaataccc acctcagata
     2281 attcttttgc tgattgattg aaaagttttc ttctctctct ggcattgaga ggattggtgt
     2341 cttgctcctc atctatagaa ctcactgcat tagttaattg ccgcatactg ggtaaagggg
     2401 taaaaccctc accactgagg cgttgtaccc gaccaccttc atgatcaaca gctaataaag
     2461 gcaagacaat ttttttatta tttgcatcta gtaagctttc ttggtatttt tttgccgttg
     2521 caaagtcaat ctcttcgcca aagtagacaa caaaaccggg ctgatattct tgaataagcg
     2581 ctaaattgtc attatctgct ggatcttgct cattatctaa aaccagaggt aacattaaca
     2641 tttgagtaag tttttctctg gtgcttaatt cgggaagatc taagttttgt tcagtttctt
     2701 cgactccttc ggtgggttgg tcaattactg gctccggaaa atactttaaa aacggttgtt
     2761 caaaataatt gtaggctaaa aagccagcca aaaaaaataa aaaaactatt aaaatattgc
     2821 gccacttcat aagttcattt tactagaatt ttattttttt ctcgcttgtg gttttttgtt
     2881 tatctacagt agttgctttt ttctttttca attggtcaag cttccacata aataatttag
     2941 ctttacttcg agcattttcc gcatcactca caaaagccaa ggctctctcc aacaaagccc
     3001 gcggataagt tttagctaat ttgatgtaaa gtgaggtatg agcttgatct cctaacacag
     3061 cagccaggta acagccgaag gcttgaaact cttgactaat atatttattg ctctctttaa
     3121 tagggtgttg gtttaataat tccgctactg tactaaattg tttttttcgc tgattactct
     3181 gcttttcgac agttttttct ttaccaaata atttggtttc atcatcatta gaattgtctt
     3241 gaaaaagaga ttgtttaacc actgtgattg attgggttgg tttttgactt ttccgttttt
     3301 taacagacat aaatgctgct tctattttaa gtcaaacacc aattaaagaa taatttgagc
     3361 taaaatttgg cgattttttt caaccagttt tgacttaaat aaaccccgct cttcaatcaa
     3421 tactttcaac ttattatctc cagccaaagt ttggtaaaaa tctaattgag tttgcagtat
     3481 ttctttggct tctttcatat tttttttacc attattggta aatgctgcca accaatcagc
     3541 actaattacg tactgcccta gattgtaaac ttcttcaccc ccagcaatcg taaaacaaac
     3601 ttgattagcc aaatgcatgt tgtgtggcct gaccatgtga cctcttactc cagattttaa
     3661 ttctttcgga tcactaaagg gcaaaatacc cacgtattcg aaatttaatc gatcggccaa
     3721 agctgctacg tgttgctgag cgctagcatt cgtttcatcc caatcattaa gctcaagatt
     3781 ttcttttaac caagcattct cttcactagc agcaaatttc aattttttat ctaactgttc
     3841 cttaggaaaa ttactatcaa tcactgtctg tagagagttg ataacttcag aaaaatgcgg
     3901 caagccaaat ccagaaccta aaaaagcgaa gcgacgacca taattgagtt ggacaccttc
     3961 atcgccatca actaccacta agggcacagg ctgaaagacg gcaggctcag ctgattgatt
     4021 gctcttactc atgaaattcc ttttgttaat taaaactact gagtcatcgc ttgcactgca
     4081 gctaaatatt gattcatctc tttttctacc tgttcccaat caccattagc ttcacgaatt
     4141 gcattaaata acagctccat gtcgattaat aattggtcgt cagtaacaat cattctttgt
     4201 tttgccatag accttcatta taccattagt cgagaaaaaa agccagtgat cggcttaatt
     4261 ttttattgaa acaccagtta ataatcgatt aaaatgttca gtttattata agcaaattga
     4321 atttatttat aagcaattgg caaatctgtc tgcacaatcg ataaactcat ccgtcgttgc
     4381 tcaggcacta cattttccac gttgaccgtc acttcatcac caacttttaa ttccttattg
     4441 ggatcaagtt tggtggaatg aattaaacca tcaacaccct tttccacgtt aacaaaaaca
     4501 ccaaatggtt caatgcgagt tactttacca gtgaaagtag ttccagcagc ataacgttct
     4561 gctaaggtac tccaggggtc ttcttctaat tgtttaattg atagatttaa tttattagtt
     4621 ctctcatcaa taccaagcac cttgactttc actcgatcac caaccttatg ataatctctg
     4681 ggatgattaa ctttttccca agaaatttcg gagatgtgaa ctaaaccctc gatataacca
     4741 gtactatctt tgccattttc gtccttgaca ggaacttcaa ccgtgacaaa caaaccaaag
     4801 tgcatcactc cagaaacaac tccttcatag atttcacctt ctcggactgc ttcaatagcc
     4861 aaatcttttt gagccaattc tttagcttca ctaacgtgtc tttcagagaa gattaaacga
     4921 tttttctcac gatcaacctc gatcgcttta actttgaaag tttcaccttt taaggaagga
     4981 agcttaccca tatactggcg accaaactgg ctagacggta caaaaccacg caccccatta
     5041 actaagacaa tcaatccgcc acgatttaat tcaacgcctt tggcttctaa gtctaaatct
     5101 ttatcaagtg cttcctcaaa atattcccat ttggcatcgg aagcggcacc tttaagagat
     5161 aatagtactt gaccattgtc gtaatcagtg gaaataacaa tacccttaat ttcatcacca
     5221 acttttaaat cttcaataaa atcattggca aattcaaatt ctttatcagc tacataggct
     5281 tcagttttag cccccaaatc aattactaaa tttttcttat ttttttcaac gataacacca
     5341 gtaaccgtgt ctcctttttg gggaacaact aaattatcag catttgcttt taatagttcc
     5401 tccattgtca tttttttggt actcttagcc atatcaaaat agttcctttc tttttgatct
     5461 gtgtattgta acacaagttt ccggttgttt cctctagaaa aagatggcaa aatagaaata
     5521 tgataaactg cccaaagata aaagacatta aaaataaaaa aattttagtt agagttgatt
     5581 ttaacgttcc tcttaaactt gtggctggaa aagctggtaa caaaaacgct gtttggcgcg
     5641 tggcggatag cgatagaatt agcagcgctc tggaaaccat caatttttta cttgatcatc
     5701 aagcaaaagt aattctaatt tcacacttag gccgagctga tggtaaaaaa aatcatgatt
     5761 taagcctttt accagtagct aaattcttaa aaaacaattt agatttacca gtagagtttg
     5821 ttaatgattg tgtgggtttt gaagttgcag aaaaatccaa aaaacttgaa gctggtcaag
     5881 ctttgttact ggaaaattta cgcttttacc ctggagagga aaaaaacgat cttaatttta
     5941 ctaaacaact aattaaagac agcggagctg aagtttacat caatgaagct tttagtgcta
     6001 gtcatcgagc tcatgccagc atagttggtt tggccaaacg tctccctagt tttgctggtt
     6061 tcaacttaaa aagagaagtt gaacaattta gtaaagctct tcagaatatc aaacggcccc
     6121 tggtggtagt aatgggcgga gccaaaatct ctgacaaagt gaccaccatg aaaaatttat
     6181 ctaatctagc tgatgtggtc ttggttggag gaggaatagc taatgccatg ctcagggctg
     6241 gaggaattga aactcaccac tcgttcattg gcgccgacga agaaaaagag ccagcagaag
     6301 tagaaaaaat ccttaaacag gcaaaagaaa ttctcagcga acatgctact gaaagaattt
     6361 tgtttgaaaa tgtcggtgag gatcgaaaaa attttccctt accaaaaatt attttaccca
     6421 ttgatgttgt cgctggtaaa gatctagatg caacaacaag tgaggtaatt accttactaa
     6481 ctgatgtggc cgacactgat catgatcggg atattaaata tctagacatt ggaccagaaa
     6541 ccattaggct ttaccgctac atcttgcaag gagccaagac tatcgtttgg aacgggccaa
     6601 tgggtgtgtt cgagaatcca ctttttgcta gagggagcag agagatagcg cgagcgatag
     6661 cgagcgccac ccaacatggg gctatttctc ttgttggtgg tggagaaagc aatagtgtca
     6721 ttaatcgagc tggtttgcac gatcaattta ctcatatttc cactgccggc ggtgctgctt
     6781 tagaatactt aagtggcgcg gagctacctg gaattaagat cttagaaaaa taatacttga
     6841 cttaagttat ttcaagaatt aataactgtt ttctatttct tgcaacaacg cccgttgttc
     6901 atcatttttt gctaatgcaa ataaatgatc aagtaaatac cacttggcct gtggctctaa
     6961 ttcaagcata ttaatcatca attgacgaaa ttcagtcaag cgattttcta aaaatagcat
     7021 atctaattgt tctctgtata aataaatata tctattgaaa tctccaacaa aatgcggcga
     7081 aattttttta aaattcaaag agaattgtac taaattattc aaatcgcttt cgtctagaaa
     7141 taaagctctt tcggcaaaaa acacatattg gtcaaactca tatgacttct cataaaaata
     7201 actagcattt gaccaatcac ctgctttata agcctgctga cccaaggcaa aaacttgctt
     7261 agctaattct tgtcttcgcc aatagctaat aaagtaatca ttagcaaaaa aatgatattt
     7321 ttcgactaat tgnnnnnnnn nnacttctct aaaagaattt tggcatcgtt ggttgaccac
     7381 tcctggaaat taatttttga aacaaaggta gcgggatagg tttctgctaa atctaaatat
     7441 aattgttttt ttaattcttg atctatttct tcatctaaat tcaaaaaccg tataataaaa
     7501 tctgaatcat aacgatataa gtgatacaaa ctttgataag tagtgttggt ataaacagta
     7561 ttatcatagg cagactttat tgcagtgtta taaaaaggcg tatatttgag ccattttttt
     7621 ggattgttct tttgccagct aattgtcaaa agatttccca ccaagaaaat tagactaagc
     7681 aggaataaaa aaagactata aatcaaccaa ctccgattac ttttgttttc gataatcggt
     7741 aaatcacgtt gttcatcttt taaaataaaa gctaaaaaaa ttaaagtcaa taaaaatatt
     7801 acaaaaaaat gccaatcaaa atcaaacatg gcattaaata atgaactact ggctcctaaa
     7861 aataaaaaat tattcaaaga ttgcttagat ttcttaatct ttaaataact ctggtgataa
     7921 agataaataa ttaaaaacac aaagagtcca ccagcgatta aacctaattc ggccaaattg
     7981 tgtaaaaaaa tgttatgagc gtaagcactt tgttgttcaa atggtaaagg aaaacgtctg
     8041 gcagcgaatt taaaagtatt taaaccataa ccaacaagag gataattttt tatcgttaag
     8101 tatccttgtc gccaataaaa aaagcgacta ttttctttca aagatataca aaaatcctta
     8161 ttataagttt ttaaaaaaca atcattgtta tctaaaaacg gtaaagacaa gaaaataaaa
     8221 attaaggagg caaatagaaa aacaaaaacc agactcttga ttagagaata aataaatgtt
     8281 ttttggcgta aattataaaa ttgctgcttg tttaataaaa gaatacaaag aaactgggca
     8341 aggaccaaca acactcctaa acgagataaa ctaatcaata ctattacata tgaggaaatt
     8401 aataaaaaaa tagtcaaata tgttgctatt tttttactaa aaagataatt tgtttttttt
     8461 ctgaataagc tccaccacaa cacgggcaaa attaaaagta aaaaagccac ataatgattg
     8521 tgaccataac tccgaactag gagattcatc ccttgaaaaa aaatcctaaa attagcatta
     8581 atggaaaaaa atataaccaa aatattcaat accaatgtta atagtagtaa gtattcgata
     8641 aaagtttcta cttttaagag ttttttatct aacttcaaaa aaaagataaa tatgctaaat
     8701 gcaaacaggt aaaacaaata tttttctatg gtgagtggga gatgatgaga aaaaacacta
     8761 gcaattagag acaataataa aaatagaaaa gataaaaaac tgattttcga aaatcgtttg
     8821 atttttaaaa aaggactata aattactaaa aagagtcccc aagcaggaaa taaatagaac
     8881 ttgtaatccg tctccatcag aaaaaaagat aataagaaaa gcaaaaaaaa gactgggtaa
     8941 aacaatttag ttaatgaatt tctcataatc aacttacttt aattcaaata tttagtatgg
     9001 ctgataaaaa aagcgaatgc aaataggatt tggccattga aataattggg gaaatttcgc
     9061 gtgatttagt ttagaattaa tttatggttc ctgcgctttt ttttatactt agcatatttc
     9121 tgcttataat gaatatctcg gatctaacac tgccttttta ttgggatact gcttggtact
     9181 tgattcctac tagcaaatta atacatgaaa atggcttact aagttttatt tcatatcctg
     9241 gtagcgatta ccctcatact tttttgttac cttccctact aagcttggct ttttatcttt
     9301 cagaaccaca agcgacaatt agtattcatc tcataggaat tcttttatcg atttttttct
     9361 tatttgttgc ctataagctg gcaaagattt ttgttgacaa aaaattagca attttaatgc
     9421 ttttgctcgt ggttagtaac ccaatttttc ttagtcaaac ctttttagtt tactttgaaa
     9481 ttattggctt agctcttcgg ttattaactt taaaattttt tcttgaaaaa aaacccaaag
     9541 cttttttaat tactagttta gtagctattt ttactcgaat tgataatttt atttttatca
     9601 gttgtatgtg tctaaatatt ttttttgacc aaaagcaaaa gattcaagaa aagattgctt
     9661 ttttaaaaaa atattattta ccttctgcaa tagttacttt tgcttggttt ttatccaata
     9721 aattaattaa tggctggtgg ttttattcac ctgaaaggta ttttgatgaa catcacctcc
     9781 aatctttcat cgaatcatta cgagttgtct ttatttatca aggcagatgg gctattactg
     9841 tggcaactat tattggctta attatcttaa ttttgcaaaa aagatggaaa ttagttttta
     9901 ataaaaaatt gttagtattt ttctctgcca cttttccaac tctaataatg attgccgttc
     9961 ttggttatgt cttgagtaga tatacgttac cattaataat ttttaattat tattttttga
    10021 tcataatctt agataaaatt caaattaaga ataacaaact tggaaaaatt gcctttatca
    10081 ttctttcttt ggcaattatt cagtttaatc ataaaaacaa ttgttttgct ggaaatcgag
    10141 aagactgtct actagtcaaa aatataatta gagaacaatt aatcaaatga ccaataaaga
    10201 tttattaatt attaaaaaaa tgtttactct accttggcct ctaaaagata aactattttg
    10261 tttattgaga tatattactg ccccttttaa tgaaatggaa aaaatggtac ccaaaaaagg
    10321 aaaaatttta gatattggct gtggacacgg cttatttgaa ttaattctaa aaagcaaatc
    10381 taataaaagg gtcatcactg ctattgatcc tgattcaaaa aaaattcaat tagctaaaaa
    10441 aattgaggaa ttatttacaa atcttaaatt tagcgactct actacaaatc tcaccaataa
    10501 taattatcag atggattgct gtattttatc tgatgtggat tatctattag atggaaaagg
    10561 gaaaacacaa attcttcaac aagcaaaatt taatttaaaa aacactggga ccattatctt
    10621 taaaactgta attaataatg gtagcattgg
//
LOCUS       DAOSEW010000013        10573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_5292_length_10573_cov_3.302434, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000013 DAOSEW010000000
VERSION     DAOSEW010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 10573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10573
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5292_length_10573_cov_3.302434"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PLJ02_00855"
     CDS             <1..383
                     /locus_tag="PLJ02_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05001.1"
                     /translation="GTLNKKEFYQFTEAESTDNNFLRTLGETGLLGFITFYGIFLVIF
                     YQIKQNQKASPWLKNLNLAFMAASIGLLANALYIDVFASSKVAFTYWALAGAILAINY
                     QQSHEAKTKQTQTQRQRLTSQKKQ"
     gene            319..1836
                     /locus_tag="PLJ02_00860"
     CDS             319..1836
                     /locus_tag="PLJ02_00860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOI05002.1"
                     /translation="MKQKLSKLRHSASGSPAKKNNSWPIIAILILAFGLRLYRLHYPL
                     LDWHSWRQADTASVTREYVKAKRVDLLQPRYHDLSNIPSGQPNPEGYRMVEFPIINGI
                     IAQFLIFSQRLGFNFDLVITSRLFSILASLLAIYALYRLIKLFDHQSLALLSAFIYAV
                     LPYAVYYGRAVLPEPFMLALTLLSLWQWAEFCRLISLKKEQPTKQSAIWLHYVLGLLA
                     LALAALLKPFVAFLAPVYLAISWFYLQQKTLKLPAVYLFALLAFLPLLLWRRWITNFP
                     AGIPASAWLFNQNHIRLRPAWWRWLFYERLTKLFLGFTGLILLFANLWQLRRVCLIYA
                     AWWLSIGLYLVVIATGNVQHDYYQNLMIPIVAISMARGSLILKEKFTNQALGNLVVTL
                     LIASSLILSAQRVAGYFNVNTWEYVEAGQAVDRLTADDALVIAPAMGDTAFLFQTNRR
                     GWPIGSAIEEKIAQGATIYVSINDDDERRALAEQYATLEVGERYLILDLTKEKEP"
     gene            1840..3039
                     /locus_tag="PLJ02_00865"
     CDS             1840..3039
                     /locus_tag="PLJ02_00865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOI05003.1"
                     /translation="MKILMLTPYLPYPLLSGGQIRTYNLLKKLANKHDITLFALIKDE
                     SETAYISELEKYCHQVRVFKRSRTPFTVKNILKTAFSSYPFLVIRNQSLTATAAIKEE
                     LANHQYDLIHAETFYMMPHLPELKIPSILVEQTIEYLGYESYAKKANPLLRPLLNIDV
                     NKIRSWEKHYWQKADQLIVMSNEDKEFIAKELGRDDKIAVVANGVDNSWFDQIPRQKT
                     KTPTILSVGTFKWLPNREAVEFLVEKIWPLVKAKIPNVRLEIVGNAPSQKVLNYGLND
                     PNINVVGQVADIRYAFKSADLLLAPVFSGKGTRYKILEAMACATPVVASQIAVEGLDV
                     ENGKHVLTSNNAQELAELTVKVLTEQKLWHTLSSNGKKFVQDHYDWQLISQKLDAIYQ
                     KIGKIKK"
     gene            3043..3921
                     /locus_tag="PLJ02_00870"
     CDS             3043..3921
                     /locus_tag="PLJ02_00870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOI05004.1"
                     /translation="MAKKLDLSIIILNYNGLFWLKKLLPTIDKFYLKKTKYRVEVIVV
                     DNDSQDDSVQFLKKNFTWVKVIESGQNGGFAFGNNVALRQTQARYVMLLNSDTEFIAD
                     KSNLDILIDYLDKHKKVAVVGPRLALSNGRLDPASHRGEPHPWAAFTYFVGLEKLFPR
                     SKFLAHYHLGHLDLDTIHPVEAVSGAAMMVKRSAINKVGLLDENFFMYGEDLDWCRRF
                     RDANYQVVFHPQVIIIHHKYKSGIKSVNKKTAKKTNSFFYNAMLTYYDKYYGPKYPHF
                     LRRILRSYIFVKRDGR"
     gene            3921..5093
                     /locus_tag="PLJ02_00875"
     CDS             3921..5093
                     /locus_tag="PLJ02_00875"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOI05005.1"
                     /translation="MTSTNKTRYRLGIDCRLAGSDNAGIGRYCENLVRETIKINQEKH
                     LFHLVLFFRNLNQAKEVLGVDYYQNPAKYHLTIELVDIRHYSLKEQLCLPKIYRRAQL
                     DLLHVPHFNIPVFYRGNLVITIHDLLWHQQKGMQVTTLKPLVYYAKYLAYLMVVRLAV
                     KRAKFILVPAHTIKDTVCHYYPQVQDKIIVTKEGIAEPYQNNLNLVSAKKLAKKRIKK
                     QLVYTGSLYPHKNIVLVLKALKKLPKYQLLVVGSRDVFQTQVRKLVARYKVKRQVRFL
                     GYVPDQKLIKLYQESLAVVQPSLSEGFGLTGIEAMASGTAVLASDIPIFHEIYQDHAF
                     YFDPKDPTSFLSALEKVEFAERESIIANNLTFAKNYRWQDLAELSCRVYLTVLQSN"
     gene            5096..6313
                     /locus_tag="PLJ02_00880"
     CDS             5096..6313
                     /locus_tag="PLJ02_00880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOI05006.1"
                     /translation="MLNSKKMAIVYDRVNTSFGGAEKVLLALHELYPQADLITSVYHP
                     RHAPWAKVFQVKTSFLQKVPGAKRWHRFFAVLMPLAFESLDLRAYDIIISVSSAEAKG
                     VITRADQLHFAYLLTPPRYLYHYQENYLQKSRFLQWPGINWLAKKALAYLKKWDYLAI
                     HRPDLIVPISYSVSTRIHKYYHLDHQPIIYPPVDVKTFQSLLANHPQPPAQTRSYYLV
                     VSRLVPYKNIDLAIKACVQLDRQLIIVGDGPESKRLQKLSRELLLAKNPKTANFKQII
                     FQKSVTDAQLSQLYQHAKALLMPGIDDFGIVALEANLHGLPVIIHQHSGASEILIDGQ
                     HALHLNYPEIMQEAHEAQYLAELCKKMQMLEQTDFDLEILTKNAIKYDTSHFIRKFNR
                     QLETAYRKQFNDR"
     gene            6303..7406
                     /locus_tag="PLJ02_00885"
     CDS             6303..7406
                     /locus_tag="PLJ02_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOI05007.1"
                     /translation="MTDNQHLYAVLLAGGGGTRLWPKSRDNYPKQFLKLTGEQSMMQI
                     AAERINRLVDWSNIIVVTNHKYVKEVSKQLPKVIKENIIAEPLKKDTALAMLVGALYA
                     KSKDKNAVIINAASDHIVTNLQEFDRVMNKAVDVAQKDKYLITVGITPTHPTSAFGYI
                     KAGQEIRKLDRDLSLFKVDSFTEKPQLATAAAFISTGHYFWNANMYVWSAKAIQKSFS
                     KYMPSLYHLTKHLEKLKTTAFHQAIKDVYQQAEAISIDYAISEKADNLVLIPGDFGWN
                     DVGDWKVVYQLGKKDPSGNVILGEEQSVQTITQKAYANLIHGNHRLIALYGIRDLIIV
                     DTDEILLICPKEHAQDVKKIVNQLKEEGRHQYL"
     gene            7428..8054
                     /locus_tag="PLJ02_00890"
     CDS             7428..8054
                     /locus_tag="PLJ02_00890"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002039990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOI05008.1"
                     /translation="MNYESKKRILDLTVASILVVLFLPFWIIIPILIVIDSGFPIFFC
                     HRRIGKDGKEFNLYKFRSMVQNADEILHNKNHELLDKFKKNDWKIAAKEDPRITRLGR
                     FLRAFTIDEFPQLYNVFRGEMSIVGPRAYVKKELKEQTEKYPETKKYVKDILSVKPGI
                     TGPWQTSGRNEIPFKQRAMMDSNYAKNRSIRQDLIILAKTPRAMISKW"
     assembly_gap    8262..8361
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8362..10325
                     /locus_tag="PLJ02_00895"
     CDS             <8362..10325
                     /locus_tag="PLJ02_00895"
                     /inference="COORDINATES: protein motif:HMM:NF024594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HOI05009.1"
                     /translation="FKGLTRKFKVSSGSKSSTHPQKKIRHLKTWQKVLLIILLIGLLL
                     LSVLASYSYFRVRKMLTLANASKTILLESADLAKTQNLPALPDKITVLKENLTALDSE
                     YQALAIYRYLPISRRYYSDGEIALQLAHQGLDLAEQTITVLLPYSDLLGFQGEGSFTG
                     GTTQDRIVLILATLEEIQPTLDDIEVKLQDIEKLSQGIDASHYPETIGDQDVRQLIID
                     AQQQITQVTQTFSDYKPIIGELGKMAGAKEAQKYLFLFQNDNELRPTGGFLTAYSIVQ
                     VDKGTVIPEKSGDIYDLDQKFWQNIPIPKELGKYLTTERYWNLRDMNIYPDFKKSMET
                     FYQYYQTIPGEPDDIDGIIAVDTQLLTWLLEVIGPVQIPGYGTFSAEVDPRCDCPQVV
                     YALSEIITRPTPYLRENRKGVLGPLMSAILSKTYELDKTKFPELFNVGINSLRGRHVQ
                     LYFVDDKSQTAAEKIAAAGRLYQLPDNFKDWQFGQNLDFLAVVDANLGGAKSNLFIDY
                     EMKLSVTAPQDGYINNTLELTYRNRAKADNCNLEAGLLCLNATLNDWIRLYLPPDSEL
                     IEIQGLEEEAEVYEEEGYQVVDGFFKLEPLGLARIRMTYRVPYQDQENYNLRLWKQGG
                     IDPVPVLIEVTGGEAALLMDQDLEYSTIF"
     gene            10328..>10573
                     /gene="recO"
                     /locus_tag="PLJ02_00900"
     CDS             10328..>10573
                     /gene="recO"
                     /locus_tag="PLJ02_00900"
                     /inference="COORDINATES: protein motif:HMM:NF023393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOI05010.1"
                     /translation="MPRTFSSQAIVLKRRAMGETDRLLTLLTQDRGKLVVLAKGARRL
                     TSSNRANLEPGNLIQAFFIETKSLTLLTQSRLLFDTCR"
BASE COUNT         3079 a         2167 c         2131 g         3096 t
ORIGIN      
        1 atggcacctt gaataaaaaa gagttttatc agtttactga agcggaaagc accgataata
       61 acttcttgcg caccttgggg gaaacgggtt tgctgggctt tattactttc tatgggattt
      121 tcttagtcat cttttaccaa attaagcaaa atcaaaaagc gagcccgtgg ttaaaaaatt
      181 taaatttggc ctttatggcc gccagtattg gactgttagc taacgcctta tatattgatg
      241 ttttcgcctc ttccaaagtg gcctttactt actgggcatt agctggcgcc attcttgcta
      301 ttaactatca acaaagtcat gaagcaaaaa ctaagcaaac tcagacacag cgccagcggc
      361 tcaccagcca aaaaaaacaa tagttggcca attatcgcca tcttaatctt ggcttttggt
      421 ctccgcctct accggttaca ttatccgttg ttggactggc atagttggcg tcaagctgat
      481 acagcctcag tcacccgcga atatgtcaaa gccaaacggg ttgatttgct tcagcctcgg
      541 tatcatgatt tgtctaatat cccttctggc cagcctaatc cagagggtta ccggatggta
      601 gagtttccga ttattaacgg catcatcgct caatttttaa tttttagtca acgtttgggg
      661 tttaattttg atttggtgat caccagtcgg ctgttttcta ttttagcctc gctcttagct
      721 atttatgccc tctatcgctt aattaaactc ttcgatcatc aatctttggc gctgttaagt
      781 gctttcattt atgcagtctt accttatgcc gtttattatg gtcgggccgt gttacccgaa
      841 ccttttatgc tggctttgac tttgctttca ctctggcagt gggctgagtt ttgccgtctc
      901 atcagtctaa aaaaagaaca gccaactaaa cagtcagcca tttggcttca ctacgtgcta
      961 ggcctgttgg cactggcact agctgcttta ctcaaacctt ttgtggcgtt cttggcccca
     1021 gtctatttgg ccatttcttg gttttatctc cagcaaaaaa ccttgaagct gcccgctgtc
     1081 tatctttttg ccctgctggc ttttttacca ctgttgttat ggcgccgttg gatcactaat
     1141 ttcccagccg gtattccggc ttcggcttgg ctatttaatc aaaaccacat tagactgcgt
     1201 ccagcctggt ggcgttggtt attttatgag cgtctgacca aactttttct cggatttacc
     1261 ggtctgattt tattgttcgc taatctttgg caactacggc gggtctgtct gatttatgct
     1321 gcttggtggc tcagtattgg gctgtatttg gtggtaattg ccacgggtaa tgtgcaacac
     1381 gattattacc agaatctgat gattcccatt gtggcgatta gtatggccag aggtagctta
     1441 attctcaagg aaaaatttac caatcaggcg ctaggtaatc tagtcgttac tttactcatt
     1501 gctagtagcc tgatcttgtc ggctcagcgc gtggccggtt attttaatgt caatacctgg
     1561 gaatatgtcg aggctggtca agcggttgat cgtctcactg cagacgatgc cttggtgatt
     1621 gctccagcca tgggcgacac ggctttctta tttcaaacta atcgtcgagg ctggccaatt
     1681 ggctcggcta tcgaggaaaa aattgctcaa ggtgctacaa tctatgtcag tattaatgat
     1741 gatgacgaac ggcgggcctt agccgagcaa tacgctactt tagaagtagg cgagcgctat
     1801 ttgattttag atttaaccaa agaaaaagaa ccataaacca tgaagatttt gatgttgacc
     1861 ccttacctgc cttatccact cctgtcgggc gggcaaattc gcacctacaa tttgctcaaa
     1921 aaattagcta ataaacacga cattactctg tttgccttaa ttaaggacga aagcgagacg
     1981 gcctacatca gtgagctgga aaaatattgt catcaagtcc gcgtttttaa acgcagtcgc
     2041 accccattca ccgtgaaaaa tattctcaag accgcttttt cttcatatcc ttttttagtg
     2101 atccgcaacc aatcattgac tgccacggcg gctattaaag aagagctagc taaccaccaa
     2161 tatgatctga ttcacgccga aaccttttac atgatgcctc acctgccaga attaaaaatt
     2221 cccagtattt tagttgaaca aaccattgaa tatttgggtt atgaaagcta cgccaaaaaa
     2281 gccaatccct tgttgcggcc gttgttaaac atcgacgtca acaaaattcg cagttgggaa
     2341 aaacactatt ggcagaaagc tgatcaactg attgtgatga gcaatgaaga taaagagttt
     2401 attgctaaag agttgggtag ggacgacaaa attgccgtag tggctaatgg cgtcgacaac
     2461 agctggtttg accagattcc tcgccaaaaa accaagacac cgactatttt atcggtcggc
     2521 actttcaaat ggttaccgaa tcgggaagca gttgaatttt tggtggaaaa aatttggccc
     2581 ctggtcaaag ctaaaatccc caatgttcgt ttagaaattg tcggtaacgc tccgagtcaa
     2641 aaagtgctta attatggtct gaatgatccc aacattaatg tcgttggcca agtagctgat
     2701 attcgctatg cgttcaagag tgccgattta ttattagcgc cagtgtttag tggtaagggc
     2761 actagatata aaattttaga agcgatggcc tgtgccaccc cggtggtggc tagccaaatc
     2821 gctgtggaag gtttagatgt cgaaaatggt aaacatgtgc tgaccagtaa taatgcccaa
     2881 gaactagccg agttaacagt caaagtctta actgagcaaa aactgtggca caccctctct
     2941 agcaatggta aaaagtttgt ccaagatcac tatgactggc aactgatttc gcaaaagctc
     3001 gatgcaattt accagaagat cggtaaaata aagaagtaag ttatggcaaa aaagcttgat
     3061 cttagcatta ttattctcaa ctataacggt ttgttttggt taaaaaaatt attaccgacg
     3121 atcgataaat tctatttgaa aaaaaccaaa taccgagtgg aagtgattgt ggtcgataat
     3181 gatagtcagg atgacagtgt gcaattctta aaaaagaatt ttacttgggt gaaagtgatt
     3241 gagtcggggc aaaatggtgg ctttgctttt ggcaacaatg tggctctgcg tcaaactcaa
     3301 gcccgttacg taatgttgct taatagcgat accgagttta ttgcagacaa aagtaattta
     3361 gatattttaa ttgattattt agataagcac aaaaaagtgg cggtggttgg ccctcgttta
     3421 gccttaagta atgggcgact agatcctgcc agtcatcgtg gtgaacctca tccttgggcc
     3481 gcttttactt attttgttgg cctggaaaaa ctctttcctc gaagtaaatt tttggctcac
     3541 tatcacttgg ggcacctaga tctagatact atccatcctg tggaggctgt ttctggtgcg
     3601 gcaatgatgg tcaaacgcag tgccatcaat aaagtcgggt tgttggatga aaacttcttc
     3661 atgtatgggg aagatttgga ttggtgccgt cgctttcgtg atgccaatta tcaagtggtt
     3721 ttccatcctc aagtaatcat tattcaccac aaatataaat ctggcattaa aagtgtcaat
     3781 aaaaaaactg ccaaaaaaac caacagtttt ttttacaatg ccatgttgac ttattacgat
     3841 aaatattatg gccctaaata tccgcatttt ttgcgccgga ttttgcgctc ttatattttt
     3901 gttaaacggg atggacgctg atgacaagca ctaataaaac tcgctatcgc ttaggtattg
     3961 attgccgact agctggcagt gacaatgctg gcattggtcg ttactgcgaa aatttggtta
     4021 gagaaactat taaaatcaat caagaaaaac atcttttcca cttagtcttg ttttttagaa
     4081 atctcaacca agctaaagag gtgttaggcg ttgattatta tcaaaatcca gccaaatatc
     4141 acttaacgat cgagctagtt gacattcggc attatagttt gaaggaacaa ctttgtttac
     4201 ctaaaattta tcgtcgcgct caactcgatc tgttgcacgt cccccatttt aatattccgg
     4261 ttttttatcg gggcaattta gtgattacca ttcacgattt actctggcat caacaaaagg
     4321 ggatgcaagt caccaccctc aaaccactag tgtactacgc caaatatctg gcctatttaa
     4381 tggtcgtcag gctagctgtt aaacgagcta aatttatttt ggttcccgcc cacaccatca
     4441 aagatactgt ctgtcactat tatccccagg ttcaagataa aattattgta accaaagagg
     4501 gcattgctga gccctatcaa aataatctca atctggtttc cgccaaaaaa ttagccaaaa
     4561 aaagaattaa aaaacagttg gtttatactg ggtcacttta tccgcacaaa aatattgttt
     4621 tagtgctcaa ggcactgaaa aaattaccca agtatcaatt gctggtagtc ggctcgcgtg
     4681 atgtttttca aactcaagtg cggaaactgg tcgcccgcta caaagtcaaa cgccaagtca
     4741 gatttttggg ctacgtcccc gatcaaaaac tgattaaact ttatcaagaa tcgctggctg
     4801 tagttcaacc ttcgttgtct gaaggttttg gcttaacggg gattgaggcc atggctagtg
     4861 gcactgccgt tttagccagc gacattccca tttttcatga aatttatcaa gatcacgcct
     4921 tttattttga ccccaaagac ccaaccagtt ttttgagcgc tttagaaaaa gtggaatttg
     4981 ctgagcgtga gtcgattatt gcaaacaatc tcacttttgc caaaaattat cgctggcaag
     5041 atctagctga attaagttgc cgcgtttatt taaccgtgct gcaatctaat taactatgct
     5101 taactctaaa aaaatggcta ttgtttatga ccgcgttaat accagttttg gtggagcgga
     5161 aaaagtgttg ctagctctgc atgagctata tccgcaagcc gatttaatca cttctgtcta
     5221 tcatccacgc cacgctccct gggcgaaagt ttttcaggtt aaaactagtt ttttgcaaaa
     5281 agtgcctggt gctaaacgct ggcatcggtt ttttgcagtg ctgatgccct tagcttttga
     5341 aagtttagat ttgcgcgcat acgacatcat tatttcggtt tctagcgctg aagccaaagg
     5401 ggtgattacc cgcgctgatc aattgcattt tgcttatctg ctgactccac cacgttatct
     5461 ttaccattat caagaaaatt atctgcaaaa aagccgtttt ttacagtggc caggcattaa
     5521 ctggctagcc aaaaaagccc tggcttacct caaaaaatgg gattatttag ctattcatcg
     5581 ccctgattta attgtgccta tttcttatag tgtctcaact cggatacata aatattatca
     5641 tttagatcat cagccaatta tttatccacc agtcgatgtc aaaacctttc agtccttgtt
     5701 ggctaaccat cctcagccac cagcgcaaac taggtcttat tacttggtgg tcagtcgctt
     5761 ggttccttat aaaaatattg atttagcaat caaagcttgc gttcaactcg accgacaatt
     5821 gatcattgtt ggcgacggtc cagagtctaa acgtctccaa aaattgagcc gagagctgtt
     5881 gctggctaaa aatcccaaga ctgctaattt caaacaaatt atttttcaaa aaagcgtcac
     5941 tgacgctcaa ctcagtcaac tttatcaaca cgctaaagcc ttattaatgc caggcattga
     6001 tgactttggc atcgtcgccc tggaagctaa tctccacggc ttgccagtga tcattcatca
     6061 acattcaggc gccagtgaaa ttttaatcga tggccaacac gcgctgcacc tgaattatcc
     6121 agaaattatg caagaagctc atgaggctca atatctggct gaactatgta aaaaaatgca
     6181 aatgctagag cagacagatt ttgatctgga aattttaact aaaaacgcta taaaatatga
     6241 tacgagtcat tttattagaa aatttaatcg acaattagaa acagcctata gaaagcagtt
     6301 taatgacaga taaccaacat ctttacgccg ttcttttagc tggtggtggc ggtaccagac
     6361 tctggccgaa atccagagac aattacccca aacagttcct caaattaacc ggtgaacaaa
     6421 gtatgatgca aattgcggct gagcgcatta accgcttggt cgattggtca aacataatcg
     6481 tggttaccaa tcacaaatac gtcaaagaag tcagcaaaca attacctaag gtgatcaaag
     6541 aaaacatcat tgctgaacca ctgaaaaagg atacagccct agcgatgctg gttggagctt
     6601 tgtatgccaa gagcaaagac aagaatgcag tgatcattaa tgccgcttcc gatcacattg
     6661 tgactaatct tcaagaattt gatcgcgtca tgaataaagc tgttgatgtc gcgcaaaagg
     6721 acaaatattt aatcactgtc ggcattacac cgactcatcc gactagtgct tttggttaca
     6781 tcaaggccgg tcaggaaatt agaaaactgg atcgtgattt aagcttgttt aaagttgaca
     6841 gttttacaga aaaaccccaa ttagccaccg ccgcggcttt tatctcgaca gggcattatt
     6901 tttggaatgc caacatgtac gtttggtcag ctaaagctat tcaaaagagc tttagtaaat
     6961 acatgccaag cttgtatcac ttgactaaac acttagaaaa attaaaaacc accgcctttc
     7021 atcaagctat taaggatgtt tatcaacagg ccgaagctat ttcgatcgac tacgccatta
     7081 gtgaaaaagc ggacaatcta gtgttgatcc ctggtgattt tggttggaac gatgttggtg
     7141 attggaaagt cgtttatcaa ctcggtaaaa aagatcctag cggtaacgtc attcttggcg
     7201 aagaacaaag tgtgcaaacg attacccaaa aagcttatgc taatctcatc catggtaatc
     7261 atcggctgat tgccctttac ggcattagag atttaattat tgtcgataca gatgaaattt
     7321 tgttaatttg tcccaaagag catgctcaag atgtgaaaaa aatagttaat caactcaaag
     7381 aggagggcag acaccagtat ctttaaaact gtaaaaaaag tttacttatg aactatgagt
     7441 caaaaaaacg cattttagat ctgaccgtcg ccagcatttt agtagtcttg tttttaccat
     7501 tttggatcat catccctatc ttaattgtta ttgattctgg ctttcccatt tttttctgcc
     7561 accggcgcat tggtaaagat ggtaaagagt tcaatttata taaatttcgt agtatggtgc
     7621 aaaatgctga tgaaattcta cacaataaaa atcatgaatt actggataaa tttaaaaaaa
     7681 acgactggaa aattgccgcc aaagaagacc cgcggattac tcgcctcgga cgctttttac
     7741 gagccttcac tatcgatgag tttcctcaac tatataacgt ctttcgtggt gagatgagca
     7801 ttgttggtcc tcgagcttac gtgaaaaaag aactcaaaga gcaaaccgaa aaatatccag
     7861 aaacgaaaaa atatgtgaaa gatattcttt cagttaaacc aggcattact ggtccttggc
     7921 aaaccagtgg tcgcaatgaa attcccttca aacaacgagc catgatggat tccaactacg
     7981 ccaaaaatcg ctcaattcgc caggatctaa ttatcttagc taagaccccc agagcgatga
     8041 tttccaagtg gtaattttgt atggttattt gttatactgt agactatgca agatttaact
     8101 agtgaggctg tacaagattt tactagtgtc gaacaattgt tcggtgacga tcagcctcaa
     8161 ttagccaatc cagaaaaaga gccattgtgg agcagtgtaa ccagtaaatt taaggggtta
     8221 actcgtaagt ttaaggtttc cagtgggtct aaaagttcaa cnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8341 nnnnnnnnnn nnnnnnnnnn naatttaagg ggttaactcg taagtttaag gtttccagtg
     8401 ggtctaaaag ttcaacacat ccacagaaaa aaattaggca cttgaaaacc tggcagaaag
     8461 ttttattaat tattttacta attggcttat tattgttgag tgttcttgcc agttatagtt
     8521 attttcgtgt acgaaaaatg ctgaccttgg ctaatgccag caaaactatt ctcctagaga
     8581 gcgctgattt agctaaaact caaaatctgc ctgctttacc agataaaatc accgtcctta
     8641 aagagaattt aactgcttta gatagcgaat atcaggcttt agccatctac cgttatctgc
     8701 caatttctcg gcgttattac agcgatggag aaattgcttt gcaactggcc catcaaggtt
     8761 tagatttagc cgagcaaact atcactgttt tactaccata tagcgattta cttggatttc
     8821 aaggagaagg aagttttacg ggcgggacga ctcaagaccg gattgtttta atcttggcaa
     8881 cactcgaaga aattcaaccc actcttgatg atattgaagt taaactacaa gatattgaga
     8941 aattgagtca aggcattgat gccagccact atccagaaac cattggcgac caagatgtcc
     9001 gccagctaat cattgacgct caacagcaaa tcacccaagt cactcagact tttagcgatt
     9061 ataaaccaat tattggcgaa ctgggtaaaa tggctggagc caaagaagca caaaagtatt
     9121 tatttttgtt tcaaaatgat aatgaactgc ggccaactgg tggtttcctc actgcttatt
     9181 ctattgtcca agttgataaa gggacggtta ttcctgaaaa atcaggagat atctatgatt
     9241 tagatcaaaa attttggcaa aacattccca ttcccaaaga attaggtaaa tatctcacca
     9301 ccgagcggta ttggaatcta cgagacatga atatttatcc tgattttaaa aaatctatgg
     9361 aaactttcta ccagtattat caaaccattc cgggtgaacc agatgatatt gatggcatca
     9421 ttgccgttga tacgcaattg ttaacttggc tcttagaagt gattggcccg gtgcaaattc
     9481 ctggctatgg tacttttagc gccgaagtcg acccacgttg cgattgcccg caagtggttt
     9541 atgcactttc agaaattatt actcgaccaa ccccttatct tcgggaaaac cgcaagggtg
     9601 tcctagggcc actgatgagt gctattttga gtaaaactta tgagctagat aaaactaaat
     9661 ttcccgaatt atttaatgta ggcatcaata gtctcagagg ccgacatgtg cagctctatt
     9721 ttgtcgatga caagtcacaa acagctgccg aaaaaattgc cgcggccggt aggctctacc
     9781 aattacctga taattttaaa gactggcaat ttggccaaaa tctagacttt ttggccgtag
     9841 ttgatgccaa tttaggtggt gccaaatcta atttatttat tgattatgaa atgaaattat
     9901 cagtcaccgc cccacaagac ggttatatta ataacacttt agaactcact tatcgcaatc
     9961 gtgccaaagc cgataattgt aacttggaag cagggttatt gtgcttaaat gctactctca
    10021 atgactggat tcgcctttat ttacccccag acagtgaatt aatcgagatt caaggtttgg
    10081 aagaagaggc agaagtctat gaagaggaag gttaccaggt ggtcgatggt ttctttaaat
    10141 tagaaccact gggtttagcc agaattcgca tgacttaccg cgtaccttat caagatcaag
    10201 aaaattacaa tctacgcctg tggaaacagg gtgggattga tccggtgccg gtcttaatcg
    10261 aagtgaccgg tggagaagca gccttgttga tggatcaaga tttagaatat tcaacaattt
    10321 tctagccatg ccgcgaacat tttctagtca agctatcgtg ttaaagcggc gcgccatggg
    10381 cgaaaccgac cgcttattaa ctttactgac ccaagatcgg ggcaaattgg tggtgctggc
    10441 gaaaggggcg cgccgcttga cttcttctaa tcgggccaat ttagaaccag gcaacttgat
    10501 tcaagccttt tttattgaaa ctaaaagcct gacgctactc acgcaaagtc gcttgctatt
    10561 tgatacctgt cgt
//
LOCUS       DAOSEW010000014        10550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_5314_length_10550_cov_4.324059, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000014 DAOSEW010000000
VERSION     DAOSEW010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 10550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10550
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5314_length_10550_cov_4.324059"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /gene="tuf"
                     /locus_tag="PLJ02_00905"
     CDS             <1..931
                     /gene="tuf"
                     /locus_tag="PLJ02_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOI05011.1"
                     /translation="ADYIKNMITGAAQMDGAILVVSATDGPMPQTREHILLANQVNVP
                     KLIVALNKCDMVDDPELLDLVEMEVRELLTKYGYDGENAPVLRVSALKALEGDKDAQD
                     GIMKLINAVDDYIPEPVRDIDKPFSMYIEDVFSIKGRGTVVTGRIESGKVKVGDTVDI
                     IGLREKKTTVVTGVEMFRKSLDQGQAGDNVGILLRGIEKDEVERGQLLAAPGTASAHK
                     KFEAEVYILKKEEGGRHKPFFSGYKPQFYIRTTDVTGEIKLPEGVEMVMPGDNAKMTV
                     ELITPVAIHEGFRFAIREGGLTVSAGAVTKIIE"
     gene            1218..1538
                     /gene="rpsJ"
                     /locus_tag="PLJ02_00910"
     CDS             1218..1538
                     /gene="rpsJ"
                     /locus_tag="PLJ02_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008933160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOI05012.1"
                     /translation="MAKNQNAYKIRVRLKSYDHRVIDEASRKILQTALSTGAKVVGPV
                     PLPTHTKSFTVLVSPHTDKDAQEHYEIRTHKRLIDIIDPTNKTIDSLMHLELPAGVDI
                     QIKM"
     gene            1588..2379
                     /gene="rplC"
                     /locus_tag="PLJ02_00915"
     CDS             1588..2379
                     /gene="rplC"
                     /locus_tag="PLJ02_00915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOI05013.1"
                     /translation="MLNTIFATKIGMSQAWSTTGKRLPITKLLVDDNCVLNVKQDQNQ
                     VFKAELAYGKKKLKNVAKPLREQISKSGFSSGFKQIRSIEVDDRVKSGETIAIDQVLE
                     LGDVVAIQGQSKGRGFAGGVKRYSFHGGPRTHGQSDRQRAPGSIGATTSPGRVWKGQR
                     MAGHFGTDTKTVLGLVVVHIDLANHEVWVNGPVPGHKNSIVKIKKTGAKKDIKLNEAV
                     FGKKEETKKEETVTLKEETPTPAPEIQVNETGEAKKKEVKEEKTA"
     gene            2379..3023
                     /gene="rplD"
                     /locus_tag="PLJ02_00920"
     CDS             2379..3023
                     /gene="rplD"
                     /locus_tag="PLJ02_00920"
                     /inference="COORDINATES: protein motif:HMM:NF012782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOI05014.1"
                     /translation="MQLPVIDSTSKNEKIRVSDILFSDKAADQLLAQAIRVYQANLRQ
                     GTSYTKTRSEVKRTKKKWYKQKGTGNARHGARTPNIFVGGGVSHGPRAIENWSLKLNR
                     KSKLIALQQALTLQKKNLFVSKDIATLKGKTKGADKLISKFSEKTDKVLVILHKMNEP
                     VLRALTNLSNVSVTTAERLNVLQMAQADKILIDFSALKTLEDRFNKVKKKSIKK"
     gene            3026..3325
                     /gene="rplW"
                     /locus_tag="PLJ02_00925"
     CDS             3026..3325
                     /gene="rplW"
                     /locus_tag="PLJ02_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006235033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOI05015.1"
                     /translation="MNAYLIKKPVITERSLSLANKQNVYTFEVLMNAHKGQIKQAVED
                     VFDVKVLEVNTVVRHRVKKKTGRKRISTVTPKTKKAYVKLAKGQTIALFDTGGNE"
     gene            3322..4161
                     /gene="rplB"
                     /locus_tag="PLJ02_00930"
     CDS             3322..4161
                     /gene="rplB"
                     /locus_tag="PLJ02_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOI05016.1"
                     /translation="MMLKKNRTGTPGTRHRVDIDRRHLHKGDPEKSLLRKGHTKRSGK
                     DAKGHISMRHRGGGVKKRQRIIDFKRNKRDIPAVVKRIEYDPMRSANIALLVYKDGEK
                     RYILAPEKLKIGDEIVAGEKVSTKTGNAMPLKNIPLGVEIHNIELRPGKGAQLVRGAG
                     TGAIIQSKEKKYATVQLPSKEFRLINLDCFATIGSLGNTDWKNRKLGKAGRKRLMGIR
                     PTVRGTAQHPDSHPHGGGEGRSGVGLKYQKTPWGKIAAPGKKTRSPRKTNKWIVRDRR
                     SKN"
     gene            4171..4455
                     /gene="rpsS"
                     /locus_tag="PLJ02_00935"
     CDS             4171..4455
                     /gene="rpsS"
                     /locus_tag="PLJ02_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOI05017.1"
                     /translation="MSRSNKKGPFVDPKLLSKVMKQKASGDKSPIKTWSRACTVAPEF
                     VGHTFLVHQGKNFVEVFVSELMVGHKLGEFAPTRTFRGHGKMVKRIMAKT"
     gene            4418..4990
                     /gene="rplV"
                     /locus_tag="PLJ02_00940"
     CDS             4418..4990
                     /gene="rplV"
                     /locus_tag="PLJ02_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOI05018.1"
                     /translation="MAKWLKELWLKLKANWIKKMIVKATQKYTRQTARKVRLVADVVK
                     EMPIESALKQLAVMERRASLVVLKTLRQAIANAMNNHQLSLDQLQIKNIVVNEAPTYK
                     RWRAVSRGRAHTILKRSCHICVELETKEPATKTEKPLEKKAEQQKTLKPADTKKSVKA
                     DIKKTSTKQSTTITKKSVKTAKKITKKENK"
     gene            4994..5644
                     /gene="rpsC"
                     /locus_tag="PLJ02_00945"
     CDS             4994..5644
                     /gene="rpsC"
                     /locus_tag="PLJ02_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOI05019.1"
                     /translation="MGQKVNPTLMRIGGIFNWKSLWFAEKDHYSDNVLEDYRLRQYLE
                     EKLSNAGVVDISIKRSISKLTLIIEVARPGVVIGKGGQNLEKIKADVDRIVNASKIKK
                     EKSRIELKIEEVKKPDLSAKLVAERIIGQLIARYPHRRAVKQAMERVMGAGAKGVKIQ
                     LSGRIDGAEISRSEKYFEGSVPTQTLRANIDYYEQPAKTRSGYVGIKVWIYKGEIV"
     gene            5661..6065
                     /gene="rplP"
                     /locus_tag="PLJ02_00950"
     CDS             5661..6065
                     /gene="rplP"
                     /locus_tag="PLJ02_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003225801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOI05020.1"
                     /translation="MLQPKKVKYRKMFRGKNRGKARHGTELAFGEFGLRAIEAGELSA
                     RQIEAARKKITFVTKREGKYWIKVFPYKPLTKKPVGVKMGSGKGAIEEYVAPVKPGFI
                     LFEVGGVSESVARSALNKAGHKLSIKTSFVKK"
     gene            6067..6276
                     /gene="rpmC"
                     /locus_tag="PLJ02_00955"
     CDS             6067..6276
                     /gene="rpmC"
                     /locus_tag="PLJ02_00955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOI05021.1"
                     /translation="MKTNEIKALHDKDQAELKKELDVLMKDLAGMRLLAKAGKLDSPA
                     KLKNLRKDVARIKAVLREKELKQTL"
     gene            6273..6512
                     /gene="rpsQ"
                     /locus_tag="PLJ02_00960"
     CDS             6273..6512
                     /gene="rpsQ"
                     /locus_tag="PLJ02_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006626562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOI05022.1"
                     /translation="MKKLIGQVLSLKNQKTASVEVARVWVHPLYGKRVKRSKKYATDY
                     DPKQMELVVGDTVEIEECRPVSKTKHFKVVKKIEK"
     gene            6515..6889
                     /gene="rplN"
                     /locus_tag="PLJ02_00965"
     CDS             6515..6889
                     /gene="rplN"
                     /locus_tag="PLJ02_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002598892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOI05023.1"
                     /translation="MIQLRSVLNVADNSGAKKIRVIQVYGGSGRRFGLVGDVVVASVI
                     SADPSGTLKKGEKVRAVIVRTVKEKRRDTGEYLRFDDNAAVIIQARDNKEPVGTRVFG
                     PVAREVKDNGFNKIASLAPEVL"
     gene            6886..7392
                     /gene="rplX"
                     /locus_tag="PLJ02_00970"
     CDS             6886..7392
                     /gene="rplX"
                     /locus_tag="PLJ02_00970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOI05024.1"
                     /translation="MKFKIGDRVLITAGKDKGKKSVIVGLLPKQNKVIVKDVNLYYKH
                     VKPFMDKPGEKRYSERPLPLAKVAILNDQDQVDRLAYRRTADGQKERFFKKTGQLAKV
                     EKVEKAVKEDKKAKKIAKKSKKEETAIVEDSQKLAQEAIKVEKKSAKKATSKLTKITR
                     IRKTQDKG"
     gene            7398..7967
                     /gene="rplE"
                     /locus_tag="PLJ02_00975"
     CDS             7398..7967
                     /gene="rplE"
                     /locus_tag="PLJ02_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOI05025.1"
                     /translation="MNRLRKQYQEKVVPALKKEFASPNLFAVPKIEKIVLNMGITDPA
                     DPKAREKIMPIIVKQFEMITGQKPVITKARQAISNFKLRQGDPLGVMVTLRGEYMWEF
                     LDKLISLVLPRVKDFRGVSATAFDSEGNYSLGLEEQIAFPEIDFDQIEKIRGLQVNIV
                     ISNSNQEKSFRLLELLGMPFEKEDIKGNK"
     gene            7971..8201
                     /locus_tag="PLJ02_00980"
     CDS             7971..8201
                     /locus_tag="PLJ02_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017428381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uS14 family ribosomal protein"
                     /protein_id="HOI05026.1"
                     /translation="MAKKSKIAKSKKLLKKRAIYLKSGERKKNRVSTRGVNRCKITGR
                     PRGYMRFFGLSRLTFRELASKGELPGVVKSSK"
     gene            8218..8607
                     /gene="rpsH"
                     /locus_tag="PLJ02_00985"
     CDS             8218..8607
                     /gene="rpsH"
                     /locus_tag="PLJ02_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011283765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOI05027.1"
                     /translation="MTDPIADMITRIKNACAAGRKDLVLPHSKVKEDIVKILKNNNYI
                     EDYQVIDKEPQKEMKIVLRYVGKLSAITNVKRVSKPGRRIYVKAGEIPQTLNGYGLTI
                     LSSNQGVMDDKTARKAKVGGELLCQIW"
     gene            8618..9154
                     /gene="rplF"
                     /locus_tag="PLJ02_00990"
     CDS             8618..9154
                     /gene="rplF"
                     /locus_tag="PLJ02_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOI05028.1"
                     /translation="MSKIGKKNISIPEQVTVTVTNDYVLVKGPKGELKTPMVEGVKVK
                     VENQEVIVERKNNLKQSRANHGLMRSLIANNVIGVTEAYRKTLKLVGTGYRVSSAGAG
                     LSMTLGFSHPVTVAAIPGITFKLEGNDTIHVEGIDKQLVGQVSANIRSLRPPEPYLGK
                     GIRYSDEVVRTKPGKTVS"
     gene            9154..9513
                     /gene="rplR"
                     /locus_tag="PLJ02_00995"
     CDS             9154..9513
                     /gene="rplR"
                     /locus_tag="PLJ02_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017381504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOI05029.1"
                     /translation="MLNIKKILNQRLKRQKRVRAKIFGTSQRPRLTVFRSNKHLYLQV
                     IDDTKGKTLAASTDFPKGKKITGTKTERTRLATEELLKILQDKKIKSLVFDRSYYKYH
                     GRIKEVADTLRKGGINL"
     gene            9519..10238
                     /gene="rpsE"
                     /locus_tag="PLJ02_01000"
     CDS             9519..10238
                     /gene="rpsE"
                     /locus_tag="PLJ02_01000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOI05030.1"
                     /translation="MARNNSRKPNQPKEFDEKVIKISRVSKKTKGGNQIGFSALVVVG
                     DHKGKVGVGLGKAGDVLSAIQKGIKKAKQKMIVVPLDGTTIPFAVTVKRGAGKVMLKP
                     ANKGTGVIAGGPVRAVVEAAGVRDVVAKILGSENQASSVHATFKALKHIADLVKIKDI
                     KLRSIAQIEKEEQEKMAALQAEAKEKAETAKKIEPKTEKKVAKKTQPKIESKAEASPK
                     KTVSKKTKPATTIKETSRSKK"
     gene            10242..>10550
                     /gene="rplO"
                     /locus_tag="PLJ02_01005"
     CDS             10242..>10550
                     /gene="rplO"
                     /locus_tag="PLJ02_01005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOI05031.1"
                     /translation="MSLFNKLEKTSSRSNKRLGRGYGSGKGGHTVGRGQKGQKSRVGS
                     KIPLWFEGGSLPLTQRLPMWRGKGRLKTLNLIAELTFNDINTIDLEEISLDTLKLNQV
                     I"
BASE COUNT         3787 a         1633 c         2332 g         2798 t
ORIGIN      
        1 cgctgattat attaaaaaca tgatcactgg tgctgctcag atggatggag caattttggt
       61 agtgtcagcg actgatggtc caatgcctca gactcgtgag cacattcttt tggctaatca
      121 agtaaatgtc cctaaactaa ttgttgcttt aaacaaatgt gacatggttg atgatccaga
      181 gttgttggac ttagtggaaa tggaagtgcg tgagttactc accaaatatg gttatgatgg
      241 tgaaaacgct ccagttctgc gagtcagtgc tctcaaagct cttgagggtg acaaggatgc
      301 ccaggatgga atcatgaagt tgatcaatgc tgttgatgat tacattcccg aaccagtgcg
      361 tgatatcgat aaaccatttt cgatgtacat tgaagatgtc ttctcgatta agggtcgtgg
      421 gacagtagtg actggtcgga ttgaaagcgg taaagttaag gttggtgata ctgttgatat
      481 tatcggtctt cgcgagaaga aaacaacagt tgtgaccggt gtggaaatgt ttagaaagag
      541 tctggatcaa ggtcaagcgg gtgacaacgt tggtatttta ctccgtggta ttgaaaaaga
      601 cgaagtcgaa cgtggtcagc tattagctgc tccaggcact gcttcagctc ataagaaatt
      661 tgaggctgaa gtctacatct taaagaaaga agaaggtgga cgtcacaaac catttttctc
      721 aggctacaag cctcaattct atatccgtac taccgatgtg actggtgaaa ttaaattacc
      781 agagggtgta gaaatggtta tgcctggtga taatgctaaa atgactgttg aattgatcac
      841 tccagtagct attcacgaag gattccgctt tgctatcaga gaaggcggtc tcactgttag
      901 tgctggtgca gtcactaaaa ttattgaata aataattcac tgattggttt ttaaagccct
      961 tgcttattgg caagggcttt tttgtttctc ttgctctttg tttttctctc tggtaaaata
     1021 cgagtctcac tataggagat aattaacagg cgctaaaggc taatatttga tttatcttct
     1081 gtacaacttg aagaaaatta gttataatcg cgctttgtga tttctacaag gaatattatt
     1141 ttctcaagta gtgatcaggt ttaaattgct aattagcaaa aaccgctaac aagtaaataa
     1201 aaaatctaaa aaatagtatg gcaaaaaatc aaaatgcgta taagattcgg gtgaggctga
     1261 agtcatatga tcaccgagtg atcgatgaag ccagtcgcaa gatcctgcaa actgctcttt
     1321 ctacaggagc caaagttgtc ggaccagttc ccttgcccac tcacaccaag tcttttactg
     1381 ttttggtttc tcctcataca gacaaggatg ctcaagagca ttatgaaata agaactcata
     1441 aacgtctcat tgatattatt gatccgacca ataaaaccat tgattcttta atgcatttag
     1501 agttgccagc aggtgtcgac attcaaatta agatgtaaat taccttaagg acttaaacaa
     1561 aattgaagag acaaaataaa agaaaagatg ttaaacacaa tattcgctac caaaatcggc
     1621 atgtcgcaag cctggtcaac aacgggcaag cgtcttccta tcactaaatt attggtggat
     1681 gataattgtg ttttgaatgt caaacaagat caaaatcaag tttttaaggc cgaactggcc
     1741 tacggtaaaa agaaattaaa aaatgtagct aaaccacttc gagaacaaat ttcaaagagt
     1801 ggtttttctt ctggttttaa gcaaattaga agtattgaag tcgatgatcg tgttaaaagt
     1861 ggagaaacaa tcgccataga tcaagttttg gaacttggtg atgttgtagc tattcaggga
     1921 caaagtaagg ggcgtggttt tgcgggagga gtcaagcgtt acagttttca tggtggtcca
     1981 cgtactcatg gtcaatctga tcgccaacgt gctcctggtt caattggtgc aactaccagt
     2041 cctggccgag tgtggaaggg gcaaagaatg gctggtcact ttggtactga caccaaaact
     2101 gttttaggtt tggtggtggt gcatattgat ttagcgaatc atgaggtttg ggtgaatggt
     2161 ccagttccag gtcataaaaa ctccattgtc aaaattaaaa aaactggagc caaaaaagac
     2221 attaaattaa atgaggccgt atttggtaaa aaagaggaaa caaaaaaaga agaaactgtc
     2281 acactaaaag aagagacgcc tactccagcg ccagaaattc aagtaaatga aactggggag
     2341 gcaaagaaaa aagaagttaa agaggaaaaa acagcttaat gcaattacca gttattgatt
     2401 ctactagcaa gaatgaaaag atcagagtaa gtgatatctt attttctgat aaagcagctg
     2461 atcaattatt ggctcaagcg attagagttt atcaagctaa tttaagacag ggtactagtt
     2521 acaccaaaac tcggagtgaa gtcaaaagaa ctaagaaaaa atggtataaa caaaaaggga
     2581 caggaaatgc tcgccatgga gccagaaccc caaacatttt tgttggtggt ggagtttctc
     2641 atggacctcg agctattgaa aactggagtt taaaattaaa tcgaaaatca aaattgattg
     2701 ctctgcagca ggcattaact ttacagaaaa agaacttatt tgtcagtaaa gatattgcga
     2761 ctttaaaggg taagaccaaa ggagcagata aattaattag taagtttagt gaaaaaacag
     2821 acaaagtttt agtaattttg cacaaaatga atgagccagt gcttcgagct ttaactaatt
     2881 taagcaatgt tagtgttact actgctgaga ggcttaatgt tttacaaatg gcgcaagcag
     2941 ataaaatttt aatcgatttt agtgctctga aaactttaga agatagattt aataaggtta
     3001 aaaaaaagag tattaaaaaa taatcatgaa cgcttactta atcaaaaaac cagttattac
     3061 tgaaagatca ctgagcttgg ccaataagca aaatgtctac acttttgagg tgttgatgaa
     3121 tgcgcataag ggtcaaatta aacaagcagt tgaagatgtt tttgatgtta aagttttgga
     3181 agttaataca gtagttaggc atcgagtcaa gaaaaaaact ggtagaaaac gaattagcac
     3241 ggtgacccca aaaaccaaga aagcctatgt gaagttagct aagggtcaaa ctattgctct
     3301 ttttgatact ggaggaaacg aataatgtta aagaaaaata gaaccggcac tcctggtacc
     3361 agacatcgag tggatattga tcgcaggcat ttgcataaag gtgatcctga aaaatcatta
     3421 ttgcgtaaag gtcataccaa acgatctgga aaagatgcca agggacacat ttcgatgcgt
     3481 catcgtggtg gtggggttaa aaaacgccaa cggattattg acttcaaacg caataagcgg
     3541 gatataccag cggtagttaa aagaattgaa tatgatccaa tgcgtagtgc taacattgct
     3601 ctgttagttt acaaagatgg tgagaaacgc tatattttgg caccagaaaa attgaaaatt
     3661 ggtgatgaga ttgtggctgg cgaaaaggtt agtaccaaaa ctggtaacgc tatgccactt
     3721 aaaaacattc ctttaggtgt tgaaattcat aatattgagt taagaccagg taagggagcg
     3781 caattagtga gaggtgcggg tactggggct attattcaat ctaaagaaaa aaaatatgcc
     3841 acagttcagt tgccttctaa agaatttaga ttgataaatc ttgattgttt cgctacaata
     3901 ggcagtcttg gaaatactga ttggaaaaat cgtaaattag gtaaagctgg acgcaaacgc
     3961 ctgatgggta ttcgtcccac tgttcgcgga accgctcagc atccagacag tcacccacat
     4021 ggtggtggtg aaggtcgcag tggagttggt ttaaaatatc agaagactcc ttggggtaaa
     4081 attgctgctc ctggtaaaaa gacgcgtagt cctaggaaga ctaataaatg gattgtacgc
     4141 gatcgtcgaa gtaaaaatta aaggaaatta atgtctagat caaataaaaa aggaccattt
     4201 gttgacccaa agcttttgag caaagtgatg aaacaaaaag cgagtggtga taagagtcca
     4261 attaaaactt ggagtagagc ttgcactgtt gctccggaat ttgtgggaca tacttttttg
     4321 gttcatcaag ggaaaaattt tgttgaagtt tttgtttcag aattgatggt tggtcataaa
     4381 ctcggagagt ttgctccaac cagaaccttt agaggtcatg gcaaaatggt taaaagaatt
     4441 atggctaaaa cttaaagcca actggattaa gaaaatgatt gttaaagcaa cacagaaata
     4501 taccagacag actgcgcgca aagttcgcct tgtggcggat gtggtcaagg agatgccaat
     4561 tgagagcgca ctcaaacaat tggcggtgat ggagcgtcga gctagcctgg tagttttaaa
     4621 aactttacgt caagccatag ccaacgcgat gaacaatcat caattaagtt tagatcaatt
     4681 acaaattaaa aacattgtag ttaacgaagc tccaacttat aagcgttggc gagcggttag
     4741 tcggggacgt gcccacacta ttttaaaaag atcatgtcat atttgtgttg aattagaaac
     4801 taaagaacca gcaactaaaa ccgagaagcc attggaaaaa aaggcagaac aacaaaagac
     4861 tctaaaacca gctgatacta aaaaaagcgt caaagctgat atcaaaaaaa ctagtactaa
     4921 acaaagcact acaattacca aaaaaagtgt gaaaacagct aaaaaaataa ctaagaaaga
     4981 aaataaataa gatatgggac aaaaagtcaa tccaactcta atgcgcatcg gagggatttt
     5041 caattggaaa tctctttggt ttgctgaaaa agatcactat tcagacaacg tgttagagga
     5101 ttatcggttg cgccaatacc tagaggaaaa actcagtaat gctggtgtgg tggatattag
     5161 tataaaacgc tcgattagta aattaacttt aattattgaa gtggctagac caggtgtagt
     5221 tattggtaaa ggtggccaaa atttggaaaa aattaaggca gatgtcgatc gaattgttaa
     5281 tgcttcaaaa attaagaaag agaaatcaag aattgaatta aaaattgaag aagtaaaaaa
     5341 gccagactta agtgctaaat tagtggctga gcgcattatt ggtcaactaa ttgctcgcta
     5401 tccccatcgt cgagcagtta agcaagccat ggaacgagtg atgggagcag gagccaaagg
     5461 agtaaaaatt caactatctg ggagaattga tggtgcagaa attagtcgta gcgaaaaata
     5521 tttcgaaggt agtgttccta ctcagactct gagagctaat attgactact atgaacaacc
     5581 tgccaaaacc agatctggtt atgttggtat taaggtgtgg atttataaag gggaaatcgt
     5641 ctaaatttgg aaattaaaaa atgttacaac caaaaaaagt aaaataccga aagatgttca
     5701 ggggtaaaaa tagaggtaag gctcgtcatg gtactgagct tgcttttggt gaatttggtt
     5761 taagagctat tgaagctggc gagttatccg ctagacaaat tgaagcggcc cgtaaaaaaa
     5821 ttacttttgt taccaaaaga gaaggaaaat actggattaa agttttccct tataaacctt
     5881 taaccaagaa accagttggt gtcaaaatgg gttcagggaa aggagcgatc gaagaatatg
     5941 tggctccagt taagcctgga tttattttgt ttgaagtagg aggagtcagt gaaagtgtag
     6001 ctcgttcagc attgaataaa gcaggacata aattatcaat taaaaccagc tttgtgaaaa
     6061 aataaaatga aaactaatga gattaaagca ctacatgata aagaccaagc agagcttaaa
     6121 aaagaacttg atgttctgat gaaggattta gctggcatgc gtttgttggc gaaggctggt
     6181 aaattagaca gtccagcaaa attaaagaat cttagaaaag atgtagctcg aattaaggct
     6241 gtattgcgtg aaaaagaatt gaaacaaaca ctatgaaaaa attaatcgga caagtgctat
     6301 cacttaagaa tcagaaaacg gctagtgttg aagtagctag agtttgggtt catcctcttt
     6361 atggtaaaag agtgaaacgt agcaaaaaat atgccactga ctacgatcca aagcaaatgg
     6421 agctggtcgt tggtgacaca gtcgaaattg aagaatgtcg accggttagt aaaactaaac
     6481 actttaaagt agtaaagaaa attgagaaat aaaaatgata caactcagaa gcgtactcaa
     6541 tgttgccgat aatagtgggg ccaagaaaat tagggtcatt caagtttatg gtggctctgg
     6601 aagaagattt ggcctagtag gagatgtggt ggtggcttca gtaatcagcg ctgatcctag
     6661 tggtactttg aaaaaaggag aaaaagttcg cgcagtgatt gtcagaactg ttaaagagaa
     6721 gagacgggat actggtgaat atttgagatt tgacgataat gctgctgtga ttattcaagc
     6781 ccgggacaat aaagaaccgg ttggcacccg tgttttcggt ccggtggctc gcgaagttaa
     6841 ggataatggt tttaataaaa ttgctagttt agctccggag gtattatgaa atttaaaata
     6901 ggagatcgag tactcatcac cgccggtaaa gataaaggca aaaaaagcgt gatagtcggt
     6961 ttattgccta aacaaaataa ggtaattgtc aaggatgtta atctttatta taaacatgtt
     7021 aagcctttca tggataagcc tggtgagaaa agatatagcg aacgaccttt gccattagca
     7081 aaagtagcta ttttaaatga tcaggatcaa gttgatcgtt tagcttatcg gcgcactgct
     7141 gatggtcaaa aggaaagatt ttttaagaaa actggccaat tagctaaagt agaaaaagta
     7201 gaaaaagcag ttaaagagga taaaaaagcg aaaaaaattg ccaaaaaatc caaaaaagaa
     7261 gaaacagcaa tagttgaaga tagtcagaaa ttagctcaag aggcaatcaa agttgagaaa
     7321 aaatcagcga aaaaggctac gagcaaattg actaaaatta ctagaatcag aaaaacacag
     7381 gataaaggtt aaaaaatatg aatcgtttaa gaaagcaata tcaggagaag gtggtgccag
     7441 ctctaaaaaa agagtttgct agtcccaatc tatttgctgt gccaaaaatt gaaaaaattg
     7501 tcttgaatat gggaataacc gatccagctg atccaaaagc cagagaaaaa attatgccta
     7561 ttatagttaa acaatttgaa atgattactg gccaaaaacc cgtgattact aaagcgcgac
     7621 aagctatttc aaattttaaa ttgcgccaag gcgatccttt aggcgtaatg gtaacactta
     7681 gaggtgaata catgtgggaa tttttagata agctgatttc tttagtttta cctcgagtaa
     7741 aggatttcag aggcgtttcg gccactgctt ttgatagtga aggtaattat agtttagggc
     7801 tagaagaaca aattgctttt ccagaaattg attttgatca aattgaaaaa atccgtggtt
     7861 tacaagttaa tatagtaatc agcaatagca atcaagaaaa atcatttcgt ctattagaat
     7921 tgctaggcat gccttttgaa aaggaagaca ttaaaggaaa taaataattt atggctaaaa
     7981 aatcaaaaat tgctaaatct aaaaagctat tgaaaaaaag agctatttac ctgaaatcag
     8041 gtgagcgtaa aaaaaatcga gtctcgactc ggggagtaaa tcgttgtaaa attaccggtc
     8101 gtcctagagg ttatatgcgc ttttttggtt taagtcgttt aacctttaga gaattagcaa
     8161 gtaaaggtga actaccaggc gtagttaaaa gcagtaaata agggacaaaa gaaatatatg
     8221 actgatccaa ttgccgatat gatcaccagg ataaaaaatg cgtgcgcagc tggacgcaaa
     8281 gatttggtac tgcctcactc taaagtaaaa gaagatatcg ttaagatact taaaaacaat
     8341 aattacattg aggattatca agttattgat aaagagccgc aaaaagagat gaagattgtg
     8401 ttgcgatacg taggtaaatt aagtgcgatt accaatgtca agagagtttc aaagcctggc
     8461 agaagaatct atgttaaggc aggtgaaatt ccgcaaactc tcaatggtta tggcttaacc
     8521 attttatcga gtaatcaagg ggtaatggat gataagacag ctcgtaaagc caaagttggc
     8581 ggtgagcttc tttgtcaaat ttggtaaata agtaaatatg tcaaaaattg gtaaaaaaaa
     8641 cattagcatt ccagaacaag taactgtcac ggtgactaat gattatgtat tagtcaaagg
     8701 ccctaaaggt gaattaaaaa ctcctatggt agaaggcgta aaagtaaagg tggaaaatca
     8761 agaagtgata gtggagcgca aaaataattt aaaacaaagt cgtgccaacc atggactcat
     8821 gcgtagtttg attgccaata atgttattgg tgtcaccgaa gcttatagaa aaaccttaaa
     8881 attagttggt actggttatc gggttagtag tgcaggagcg ggtttgagta tgactctggg
     8941 tttttctcat ccagtaactg ttgctgcaat accagggatt acttttaaac ttgaaggtaa
     9001 cgacactatt catgttgagg gtattgataa acagctagtg gggcaagtaa gtgctaatat
     9061 tcgctctttg agaccgcccg aaccatattt gggcaaaggt attcgttaca gtgatgaagt
     9121 agtaagaact aaaccaggaa agactgtctc ttaatgttaa atatcaaaaa aattcttaac
     9181 cagcgtttga aaaggcaaaa gcgagtgcga gctaagattt ttggcactag tcaacgacct
     9241 cgtttaaccg tttttcgatc caataagcat ttgtacttac aagtgattga cgatacaaaa
     9301 ggaaaaactt tagcggctag tacggatttt cccaagggta aaaaaatcac tggtaccaaa
     9361 actgaacgaa cccgattggc tactgaagaa ttattaaaaa ttttgcaaga taagaaaatc
     9421 aaatctctag tttttgatcg ctcatactat aaatatcatg gacgcattaa ggaagtagct
     9481 gacactttaa gaaaaggcgg tattaatctt taaaacatat ggcaagaaat aattcaagaa
     9541 aacccaatca acctaaggaa tttgacgaaa aagtcattaa aatttctcgg gtctctaaaa
     9601 aaaccaaagg tgggaaccag attggttttt ctgctctcgt cgtggttgga gatcataagg
     9661 gtaaagtagg tgtaggcttg ggtaaagctg gtgatgtttt gtcagctatc caaaaaggga
     9721 ttaaaaaagc taaacagaaa atgattgttg ttcctttaga tggaaccacc attccttttg
     9781 cagtaaccgt taaaagaggt gctggtaagg tgatgttaaa accagctaac aaaggaacag
     9841 gagtaattgc tggaggtcca gttagagcag tggttgaagc ggccggggtg agagatgtgg
     9901 tagctaaaat tctaggtagt gaaaatcaag cttccagtgt tcatgccact ttcaaagctc
     9961 ttaaacacat tgccgactta gtgaaaatca aagatattaa attgcgctca atcgctcaaa
    10021 ttgaaaaaga agagcaagaa aaaatggcag cgctacaagc ggaagctaaa gaaaaagcag
    10081 aaactgctaa aaaaattgag ccaaagaccg aaaaaaaagt cgccaaaaaa actcaaccaa
    10141 aaattgagtc taaagccgaa gcaagtccta aaaaaactgt ttccaaaaaa accaagccag
    10201 caacaactat taaagaaaca agtagaagta aaaagtaaac aatgtcatta tttaataaac
    10261 ttgaaaaaac tagttctaga tccaataagc gcctcggacg aggttatggt tctggtaaag
    10321 gtgggcacac ggttgggcgt ggtcaaaagg gccaaaaatc tcgagttgga tccaaaattc
    10381 ctttatggtt tgaaggaggc tcgttgcctc ttactcagcg tctaccgatg tggcgtggca
    10441 aaggtcggct caaaacttta aacctgattg cagagttaac tttcaacgat ataaatacca
    10501 ttgatttaga agaaataagc ttggatactt taaaattaaa ccaagtgatt
//
LOCUS       DAOSEW010000015        10332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_5480_length_10332_cov_4.471246, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000015 DAOSEW010000000
VERSION     DAOSEW010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 10332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10332
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5480_length_10332_cov_4.471246"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1427)
                     /gene="dnaK"
                     /locus_tag="PLJ02_01010"
     CDS             complement(<1..1427)
                     /gene="dnaK"
                     /locus_tag="PLJ02_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005979893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOI05032.1"
                     /translation="MSKTIGIDLGTTNSAVAVMQGGQPTIIPSAEGRNTFPSIVAIKN
                     KDIVVGDPAKRQMVLNPKTTVYSVKRFIGRKFKDEAVQKAIKHVAYDIVEGKDGMAVV
                     KINNKEYTPQEISAKILAKAKTDAESYLGEKVESAVITVPAYFDDSQRQATKQAGEIA
                     GLKVERIVNEPTAAALAYGLDKKGSKTIAVYDLGGGTFDISILEIGDGVFEVKSTNGD
                     TFLGGDDFDNAIIEWIVAEFKKDTGKDLSKDSQAMQRIKDSAEKAKIELSSSQQTEIN
                     QPFIAQGDEGQPLHLSLTLTRAKLEELVDDLIQKSIGPVKKAMKDAKMDVKDIDEVIL
                     VGGMTRMPKVQEVVKDFFGKEPHKGVNPDEVVAVGAAVQAGVIAGDVTDVVLLDVTPL
                     TLGLETLGGIRTPLIDRNTTIPTSKSQIFSTAADNQTQVEINVLQGEREMAADNKSLG
                     RFILDGIPPAPRGIPQIEVTFDIDS"
     gene            complement(1577..2041)
                     /gene="grpE"
                     /locus_tag="PLJ02_01015"
     CDS             complement(1577..2041)
                     /gene="grpE"
                     /locus_tag="PLJ02_01015"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOI05033.1"
                     /translation="MAKKKTAKTDSSLEVNKLTAQVHDLEQALWQSQERERRALADYQ
                     NFQRQTQQQRANLLKLANADLLLTILEPLEHLSTASEHIQDEALTMIINQLWKRLKEI
                     GLEEIEVLGKKFDLETMEAVEKKGNGDKVIKVLRKGYRLNGEIIQHAQVVLG"
     gene            complement(2056..2778)
                     /locus_tag="PLJ02_01020"
     CDS             complement(2056..2778)
                     /locus_tag="PLJ02_01020"
                     /inference="COORDINATES: protein motif:HMM:NF013768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05034.1"
                     /translation="MIDLVARQIQILRAIIEEFIETGEPVGSETVDKKYNIGVSPATI
                     RNEMAYLTKQGYLLKSHISAGRVPTALAMKLYINELVKEKELSVADEVSIKEKIWRYR
                     NQMEDLLRETARILADKSQALGLAVTPDGNIVHSGYANLLEMPEFYDINLTRRVLQMI
                     EERSVMDEIFEKSQSENPVKVIFGQELGNRELDPIGIMFVELEMPEHPCRFAVLGSTR
                     FDYPYVLPMMKYFKGLIESMID"
     gene            complement(2813..3925)
                     /locus_tag="PLJ02_01025"
     CDS             complement(2813..3925)
                     /locus_tag="PLJ02_01025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOI05035.1"
                     /translation="MAKVALVHDYLREYGGAERVLEELHRLYPEAPVYTAFVDQKSLG
                     ENWQRFQDWDIRQTFLSKIPLIKKTFSPLRVLAARAFAQLDLSEFDIVISSSNAYMAK
                     AARASKPHAKHFCYCHTPSRVLYGYTAKSHWRDRAITRFFGEIINHYMRFVDFRVAQE
                     VGVFIANSEETQARIKKFYHRQSVVIYPPVKMVDTAKDYLAKHQSFKKEGYYLYVNRL
                     AYAKHPEIAVAVATDLNLALKVVGDGAMLSELKHMAGKSVEFLGAVDDQTLADLYRGA
                     KALIYPVEDEDFGMVPIEAMAWGTPVLAHYSGGPKETIQNAKTGLFFQELSAEALKQA
                     IFDFEKMDFEAKEIHLSSLIYSPSAFRKKIKALVQT"
     gene            complement(4009..4938)
                     /locus_tag="PLJ02_01030"
     CDS             complement(4009..4938)
                     /locus_tag="PLJ02_01030"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HOI05036.1"
                     /translation="MSRQIFLLVDAHALIYRAYYALPNLTDPEGHLVNAAYGFTRMFL
                     TAINHFEPEYSAVCFDHKGETLRKKHYEAYKAQRAPMPDDLIPQIALVKELVDALNVP
                     RFEIEGYEADDLIGTINRQVEHLDGRLLTVIVTGDQDMFQLVDNDTHVWMPARGKQQG
                     DMEYDRRLVEKKLQIKPSQIVDFKALSGDASDNIPGVKGIGKKTATKLIHRFASLEQI
                     YAFLDKNPTGDDLVKGALLTKLKEGKASAFLSQDLATISTEVDLDFSLEACRVTAYDK
                     TKAIQLLNKLGFKSLIKLLPTDAFEHSVQEALF"
     gene            complement(4967..7000)
                     /gene="ftsH"
                     /locus_tag="PLJ02_01035"
     CDS             complement(4967..7000)
                     /gene="ftsH"
                     /locus_tag="PLJ02_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOI05037.1"
                     /translation="MFKNKNSDQPLTTDSGKTKSGSKTSNQETKPDKEQLAKEKLARE
                     KAAQELADRMEQIQLRMPIVKSSKKKVIKINLNRLISRTIIYIVILFLFLPSLLNLLG
                     LNQAREQISVSQMVRDVRDGQVKNIEVLGQELRLSYADGSQKVTRKEEGQQALEILFA
                     SDIDLAALDLEVQDASFGQLFWELVINFLPIILMFVFFIMIFRQAQSGQEGILGIGKS
                     KAKVFIKGKQDISFKDVGGMEEAKRELEEIVDFLKNPKKYQKVGARTPKGVLLVGPAG
                     TGKTLLARAVAGEANVQFLSIAGSEFMEMLVGVGASRVRDLFETAKRLAPSIIFIDEI
                     DAIGRARGRNSFSSHDEREQTLNQILVEMDGFTKNDNVIVMAATNRGDMLDPALVRPG
                     RFDRRVQVTLPDLKERQFILKIHARNKTFNADVSWEKVAKRTVGFSGADLENMLNEAA
                     ISAARESRQKINMEDIEEASMKVKYGPSKKRLLDKLEKKMTAYHEAGHAVLSHVLPYT
                     DPVHRISIVSRGQALGYTFTPPEKDKLQVLKSELLENMIVMFGGRAAEMLVFNEQTAG
                     AASDISQATRIARAMVVRYGMSKLGPMYLGPEYTDNDYNRMWDEPERPSDKLAQAVDE
                     EILALIKEAENKALQLLKKYRTQLDNVAEALLEKETLDSDEFTKVMGMEKARR"
     gene            complement(7049..8107)
                     /gene="trpS"
                     /locus_tag="PLJ02_01040"
     CDS             complement(7049..8107)
                     /gene="trpS"
                     /locus_tag="PLJ02_01040"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOI05038.1"
                     /translation="MSKTKKRVLSGIRASGSLHLGNYFGAIKGMLALQEDRDVETFYM
                     VADAHAITTPYQVEELRANRLSVILDYLAVGLDPEKSVLFFQSAVPQHFELAFYLSTL
                     ISVAKMQHLPTFKDKVKQHPENVTMALLSYPVLMAADILLYKANLVPVGVDQKPHIEV
                     AREIARKINGLYGTQFPIPKFFNTTGEYVPSLTGEGKMSKSVPGSYINLDDDLATIEK
                     KVARIPTDSGKGEIRKLEAEGDQHHDLVYIDDHGQESKGVKTLMILVELFAGKASRKE
                     YEAAYQKNGLRYATIKKDLAKLIYQELQPIQERRRELVKKKDYLADVIATGNQQASMV
                     ARATMAEVKEKFALSTVV"
     gene            complement(8162..9028)
                     /locus_tag="PLJ02_01045"
     CDS             complement(8162..9028)
                     /locus_tag="PLJ02_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOI05039.1"
                     /translation="MPINLNDAQVKFTEHLKASGKADSTVIAYSKDVEQLLDFVAKRG
                     KTTISEILPEDIDEFKELLNKQRYTSKSISRKINSIKSFFRFLIGEGLVDKNPTTNIS
                     HPKIEQSSPRVLSRLEYRALRDACRGDSRMYAIVELLLQTGMRISELAALTITDLDME
                     REIIHIQAQNSRDARRVPMNTAAKNAILDYLKVRPRAKEKTLFLTKTCRPFLVRNIRT
                     AIDRFFRLAGIKDAKVNDLRHTFIVEQLKAGTPLVYVSQLVGHKRITTTEKYLKLIEA
                     PDMQPNVKIEEL"
     gene            9134..9520
                     /locus_tag="PLJ02_01050"
     CDS             9134..9520
                     /locus_tag="PLJ02_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05040.1"
                     /translation="MAKNKRQLLEQPLMIVLISFLAILLMLSLQESKRKAALDSKNLE
                     QNRQNIALLEKSVTDKEAFFQQTQSDLYKEKILRNELIKNKAGETVIQIEKTDFNPII
                     EEENNVNPSDYRLANWQAWWQLLVKN"
     gene            9538..10302
                     /locus_tag="PLJ02_01055"
     CDS             9538..10302
                     /locus_tag="PLJ02_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOI05041.1"
                     /translation="MQYFNFEGQTALVTGASSGLGKQFALLLASAGADVAIAARREDK
                     LKEVAEEIEALGRKALILVMDVTKEEEIAAGFAKIKKEWQKLDIVVNNAGISSAAPAE
                     EMSFEDWQRVVDVNLTGVFLVAKHAGALMKEKKYGRIINLASIYGLVGNSAFNVANYH
                     ATKGAVVNLTRALAAEWAKYQITVNAIGPGFFESEMTGDFINTDDFKQYVAISCPMKR
                     VGKKGELNTALLFLADKASSYVTGQTIAVDGGWTAI"
BASE COUNT         3086 a         2218 c         1784 g         3244 t
ORIGIN      
        1 ttactatcaa tgtcaaaagt gacttcaatc tgtggtatcc ctctcggtgc tggtggaata
       61 ccatccaaga taaagcgacc taggctctta ttatcagctg ccatttcgcg ctcaccttgc
      121 aacacattga tttctacttg agtctgatta tcagccgcag tggaaaagat ctggctttta
      181 ctggtgggaa tagtcgtatt acgatcaatc aaaggagtgc gaataccacc caaagtttcc
      241 aaacctaaag ttaatggagt aacatccagc aataccacat cagtgacatc tccagcaatg
      301 actccagcct gaacagctgc acccactgcc actacttcat ctggattgac tcccttatgc
      361 ggttctttgc caaagaaatc tttgaccact tcttgaacct taggcatcct ggtcatacca
      421 cccaccaaaa ttacttcatc aatgtctttg acatccatct tggcatcttt cattgccttt
      481 ttaactggac caatcgattt ttgaattaaa tcatcaacca attcttccaa tttagctctg
      541 gttaaagtta aactcagatg caatggttgc ccctcatcgc cttgagcaat aaagggttga
      601 ttaatttctg tctgttgaga actggataat tcaatcttag ctttttctgc agagtctttg
      661 atccgttgca tcgcctgaga atctttgctg agatccttac ctgtatcttt tttaaattcg
      721 gcaactatcc attcgataat agcgttatca aaatcatccc ctcccaaaaa agtatcacca
      781 ttggttgatt tcacttcaaa cacgccatca cctatctcta aaatagaaat atcaaaagta
      841 ccaccaccca aatcatagac agcaatggtt ttactgcctt ttttatctaa accgtaggcc
      901 aaggccgctg ccgtgggctc attgacaatg cgctcaacct ttagaccagc aatttcaccg
      961 gcttgtttag tagcttgtcg ttgagaatca tcaaaataag cgggcactgt aattactgct
     1021 gattcaactt tttcacctaa ataactttca gcatcagttt tggctttagc taaaatcttg
     1081 gccgaaattt cttgcggggt gtattcttta ttattaattt taacgacagc cataccatct
     1141 ttgccctcga ctatatcgta ggcaacgtgc ttaatggctt tttgcactgc ttcatcctta
     1201 aatttgcgac ctataaaacg tttaaccgaa taaacggtag ttttgggatt taacaccatc
     1261 tgtcttttgg caggatcacc caccactata tctttatttt tgatggcgac aatggaggga
     1321 aaagtattac gaccctcagc actaggaata atggtcggtt gaccaccttg catcacagca
     1381 acggcactat tggttgtccc caaatcaatt ccgattgttt ttgacataat tgataaacct
     1441 ttctataaat tgttgttttt attgttaatt cttgttgatt aatttattta attgttaatt
     1501 actaaacttt tgtaacttta gttaatgcaa gcaccgtcaa ttttagcaga caacagcgtt
     1561 gactgctagt agcaatttac cctaagacca cttgggcgtg ttgaataatt tccccgttta
     1621 aacgataacc tttgcgtaaa actttaatga ctttatctcc attacctttt ttctctaccg
     1681 cttccatagt ttctaaatca aatttcttac ctaaaacttc aatttcctct aaacctatct
     1741 cttttaggcg cttccataac tgattgataa tcattgttag tgcctcgtct tgtatatgtt
     1801 cactggctgt ggataggtgt tctagcggct ctaaaatggt tagtaataaa tctgcgttgg
     1861 ctagtttcag caaatttgcc cgttgttgtt gtgtctgacg ttgaaaattt tggtaatctg
     1921 ctaaagctcg acgctctctc tcttgagact gccacaaagc ttgctctaaa tcatgcactt
     1981 gagcagttaa tttattcact tccaaagatg aatcggtttt agcggttttt ttctttgcca
     2041 tttctagatc actttctaat caatcatact ttcaattaaa cctttaaaat atttcatcat
     2101 tggcaagaca tatgggtaat caaagcgtgt ggaacccagt accgcaaagc ggcaggggtg
     2161 ctccggcatc tccaactcca caaacataat gccgattgga tctaattctc tattacctaa
     2221 ttcttggcca aaaatgactt taactggatt ttcactttgc gatttttcaa aaatctcatc
     2281 catcaccgaa cgctcttcaa tcatctgtaa cacccggcga gttaagttga tgtcataaaa
     2341 ctctggcatc tccagcaaat tagcataccc agaatggaca atattaccat ctggagtcac
     2401 agctaaacct aaagcttgcg atttatctgc caatattctt gctgtctcac gcagtagatc
     2461 ttccatttga ttgcggtagc gccaaatttt ttccttaata ctgacttcat cggccactga
     2521 taactctttt tctttaacca actcattgat atacaacttc atcgctaaag ccgttggcac
     2581 ccgaccagca ctaatatgag atttaagcaa ataaccctgc ttagtaagat aagccatttc
     2641 attacggata gtcgctggag aaacaccaat attatatttt ttatcaactg tctctgaacc
     2701 tacaggctct ccggtttcaa taaattcttc aataatggct cttaaaattt ggatttgtct
     2761 ggcaactaga tcaatcatat attagcaatc atagaaatta attgctaatt tgtcaagttt
     2821 gaactagtgc cttaattttt ttgcgaaaag ccgacggaga ataaattaag ctggataaat
     2881 gaatctcttt agcctcaaag tccatttttt caaaatcaaa aatggcttgc ttcagcgcct
     2941 cagcacttag ctcttgaaaa aacaaaccag tcttggcatt ttggatggtc tctttcggac
     3001 ctccagaata atgagctaaa actggcgtgc cccaagccat cgcctcaatt ggcaccatgc
     3061 caaaatcttc atcctcaact ggataaatta aggctttcgc tcctcgatac aaatcagcca
     3121 aagtctgatc atccactgca cccaaaaatt cgacagattt accagccatg tgttttagtt
     3181 cgcttaacat ggctccatcg ccaactactt tgagggctaa attaagatca gtcgccactg
     3241 ccactgctat ttcaggatgt ttggcatagg ctaagcgatt cacgtacaag taataaccct
     3301 ctttcttaaa actctgatgt ttggccaaat aatccttggc cgtatccacc attttcactg
     3361 ggggataaat cactacagat tgccgatgat aaaatttttt gattctggct tgggtttcct
     3421 ctgaattagc aataaatacc cctacttctt gggcaactct aaagtcaaca aagcgcatgt
     3481 aatgattgat aatttctcca aaaaaacgag taatggctcg atcacgccaa tgacttttag
     3541 cggtatatcc atataaaact cgtgatggcg tgtgacagta gcagaaatgt ttggcatgag
     3601 gcttactggc gcgcgccgct ttggccatat aggcgtttga agaagaaatc acaatgtcaa
     3661 attcactgag atctaattga gcaaaggcgc gcgccgctaa taccctcaaa ggtgaaaaag
     3721 tttttttaat tagcggaatt ttactcaaaa aagtttgacg aatatcccaa tcctgaaaac
     3781 gttgccaatt ttcccctagt gatttttgat caacaaaagc ggtataaacc ggagcttcag
     3841 gataaagtcg atgcaactcc tccaacactc gctctgcgcc accatattct cttaaataat
     3901 catgaactaa agcgacttta gccatatttt ttatccttag ttgattttaa attcgaattt
     3961 tttctaattt ttttctcatg cttttcttat gcttaattta atatcattct aaaataatgc
     4021 ctcttggact gaatgctcaa aagcgtcggt tggtaataat ttaatgagag atttaaaacc
     4081 caatttattt aataattgaa tagctttggt cttgtcgtag gcggtgacgc gacaagcttc
     4141 taaagaaaaa tcgagatcaa cttcggtact aattgtagcc aaatcctgac tcaaaaaggc
     4201 agatgctttg ccttccttta attttgttaa taaggcacct ttgactaaat catcacctgt
     4261 tggattttta tctaaaaacg cataaatttg ttctaaacta gcaaagcgat gaatcaactt
     4321 agtggcagtt ttcttaccaa tacccttaac acctggaata ttgtccgaag catcaccgct
     4381 caacgcttta aaatccacta tctgactcgg cttaatctgc aacttttttt ccactagtct
     4441 gcgatcgtac tccatatcac cttgttgttt acctctagct ggcatccaaa cgtgggtgtc
     4501 gttatcgacc aattgaaaca tatcttgatc accagtcaca atcactgtta ataaacgacc
     4561 gtctaagtgc tccacctggc gattaatggt accaattaaa tcatcagcct cataaccttc
     4621 tatttcaaaa cggggaacat tgagcgcatc aactaattct ttgactaaag caatttgagg
     4681 aattaggtca tcgggcattg gcgctcgctg agctttataa gcttcataat gtttttttct
     4741 tagagtttct cctttgtgat caaaacaaac ggcactgtac tccggctcaa aatgattaat
     4801 ggcagtcaaa aacatccggg taaaaccgta ggcagcattg accaaatggc cctcaggatc
     4861 agtcaaatta ggtagtgcat agtaagcacg ataaattagg gcgtgcgcat caactaaaag
     4921 aaaaatttga cgagacatgt gcttattgta acaaaggcaa taatatttat ctcctagctt
     4981 tttccattcc cataaccttg gtgaattcat ctgaatccaa agtctctttt tccaataatg
     5041 cttcggctac attatccaac tgtgttcgat atttctttaa caattgtaaa gctttattct
     5101 ccgcttcttt aatcagcgcc aaaatttctt catcaacagc ttgagccaac ttatctgacg
     5161 gtcgctcagg ttcatcccac atgcgattgt aatcattgtc agtatattct ggacccagat
     5221 acataggacc taatttactc attccataac ggaccaccat ggcacgagca atgcgagtgg
     5281 cttgtgaaat atcgctggcc gctccagcag tttgttcatt aaaaaccagc atttctgccg
     5341 cccgaccacc aaacatcaca atcatatttt ctaacaattc tgatttgagc acttgtaatt
     5401 tatctttttc tggaggagta aaagtataac ccaacgcctg acctcgagaa acaatggaaa
     5461 tacgatgcac tggatcagta tatggcaaaa catgacttaa cacggcatga ccagcttcat
     5521 gataagcagt cattttcttc tctaatttgt ctaatagacg cttcttgctt ggaccatatt
     5581 taaccttcat agaagcttct tcaatatctt ccatattgat cttctggcga ctttctctgg
     5641 ctgccgaaat agctgcttca tttaacatat tttctagatc tgctccagaa aaaccaactg
     5701 ttcttttagc gactttttcc caagaaacat cggcattaaa agtcttattg cgggcgtgaa
     5761 tttttagaat aaattgtctt tcttttagat ctggtaaagt cacttgcaca cgtcgatcaa
     5821 aacgacctgg tcttaccaga gcaggatcca acatgtcccc acggttggtg gctgccataa
     5881 caattacatt atcattttta gtaaaaccat ccatctcgac taaaatttga ttcaacgtct
     5941 gttctctctc gtcgtgggaa ctaaaactat tacgccctct agctctaccg atggcatcaa
     6001 tctcatcaat aaaaataatg gatggagcta aacgtttggc tgtttcaaat aagtccctga
     6061 ctcgtgaagc gccaacaccg actaacattt ccataaattc gctaccagcg attgataaaa
     6121 attgcacgtt agcttctcca gccaccgcgc gagctaacaa agttttaccc gtaccagcag
     6181 gaccaactaa taacacacct tttggcgttc gcgcaccaac tttttgatat ttcttgggat
     6241 tctttaaaaa atcaacaatc tcttccaatt cccgtttggc ttcttccatc ccaccgacat
     6301 ctttgaaact gatatcttgt ttacctttaa taaaaacttt ggccttgctt ttgccgatac
     6361 ccaaaattcc ttcttgacca ctttgcgcct gcctaaaaat catgataaag aacacaaaca
     6421 ttaaaatgat cggtaagaaa ttaattacta attcccaaaa taattgacca aaactagcat
     6481 cttggacctc caaatccaag gcagctaaat caatatcgct agcaaaaaga atttccagag
     6541 cttgttggcc ctcttctttt ctcgttactt tttgggaacc atcagcataa gataagcgca
     6601 attcttgacc tagcacttca atatttttta cctgaccatc cctgacatct ctgaccatct
     6661 gactaacgga aatttgttct ctagcttgat ttaaacccaa gaggttcaaa agtgagggca
     6721 aaaataaaaa caaaattact atataaataa tagttcgtga aattaagcga tttaaattaa
     6781 ttttaatgac ttttttcttg ctacttttga ctataggcat ccggagctga atttgttcca
     6841 tccgatcagc caattcttga gcagcttttt cgcgagctag tttttctttc gccaattgtt
     6901 ctttgtcagg cttagtttct tgattactag ttttcgaacc tgatttggtt ttgcctgaat
     6961 ctgtcgttag tggttggtct gaatttttat tcttaaacat gaatccttat ttagtgatac
     7021 tgatatcttt gggagtacta ttgtagcatt aaacaacagt actcaaagca aatttttctt
     7081 taacttctgc catggttgct ctagcgacca tactagcttg ttgattgccg gttgcaatga
     7141 catcggccaa atagtctttt ttcttaacta actctcgtct tctttcttga attggctgaa
     7201 gttcttgata gattaatttg gctaaatcct ttttgatggt ggcgtagcgc aaaccatttt
     7261 tttgataagc agcctcatat tcttttctac tagctttgcc tgcaaaaagc tcaactagaa
     7321 tcatcaaagt tttcactcct tttgattctt gaccatgatc atcaatataa actaaatcat
     7381 gatgttgatc gccctctgct tctagctttc taatttcacc ctttccagaa tcggtaggaa
     7441 ttctagccac ttttttctca atagtagcta aatcatcatc taaattaata taactgccag
     7501 gaactgactt gctcatcttg ccttcaccgg taagcgaagg cacatattcg ccggtagtat
     7561 taaagaactt agggatggga aattgagtgc cataaagacc attaattttg cgcgcaattt
     7621 ctctagccac ttcaatgtgt ggtttttgat caactccgac tggtactaaa ttggctttat
     7681 ataacaaaat atcagccgcc attaacactg gatagcttaa cagcgccatc gtgacatttt
     7741 ctggatgttg tttaacttta tctttaaaag ttggcaaatg ttgcattttg gctaccgaaa
     7801 ttaaagtaga taaatagaaa gccagttcaa aatgctgagg tactgctgat tgaaaaaata
     7861 agacactttt ttctggatct aaacccaccg ccaaataatc tagaataaca cttaaacgat
     7921 tggcgcgtaa ttcttcaact tgataaggag tagtgatggc gtgggcatca gccaccatat
     7981 aaaaagtctc aacatcgcga tcttcttgaa gggccaacat ccctttgatg gcaccaaaat
     8041 aattgccaag atgtaaacta ccacttgccc gaataccaga taaaactctc tttttagttt
     8101 ttgacataac acctctaatc ttaacacaag ccaagaatta ttgggataaa aaatgcttaa
     8161 attaaagttc ctcgattttg acgttcggtt gcatatccgg cgcttcaatc agttttagat
     8221 atttttctgt ggtggtaatg cgcttgtggc cgaccaattg agaaacataa actaatggcg
     8281 taccggcttt aagctgttcc acaataaaag tgtgacgtaa atcattaacc ttggcatctt
     8341 tgatgccagc caaacggaaa aaacgatcaa tcgctgtgcg aatatttctc actaaaaaag
     8401 gacgacaagt tttagtcaaa aataaagtct tttctttggc tcgaggacga acttttaaat
     8461 aatctaaaat ggcatttttg gcagcagtat tcattggcac tcgacgagca tctcgtgagt
     8521 tttgggcttg aatatgaatg atttctctct ccatatccag atcggtaata gttaaagccg
     8581 ctagttcact aatgcgcatg ccggtttgta aaagcaattc aacaatggca tacattctag
     8641 aatcacctcg gcaagcatct ctcagcgctc gatattcgag acgagacaat actctcggag
     8701 aactttgttc aattttggga tgactaatgt tagtagtggg atttttgtca actaaacctt
     8761 caccaattaa aaaccgaaaa aatgatttaa tcgaattaat tttgcgtgaa attgacttac
     8821 tagtgtaacg ttgtttattt aataattcct taaactcatc aatatcttca ggcaaaattt
     8881 cactgatagt ggttttgcct cgcttcgcca caaaatcaag cagttgctca acgtctttac
     8941 tgtaagcaat gactgtcgaa tcagcctttc cactggcttt aagatgctca gtaaatttaa
     9001 cttgcgcatc atttaagttt atgggcataa tatgtctaat ctaggacaga ctaaaaaatt
     9061 ataatttttt taatgattga gggtcttata gtatcataaa ttaggatttt tttcaatctt
     9121 gattaacaag attatggcaa aaaataagcg acaacttcta gaacagccat taatgattgt
     9181 actgattagt tttttggcta ttctcttaat gctttccctg caagaaagta aacgaaaagc
     9241 cgcactggat agtaaaaatc ttgaacaaaa tcgccaaaat attgctcttt tagaaaagag
     9301 tgttaccgac aaagaagctt ttttccaaca aacgcaaagc gacctatata aagaaaaaat
     9361 tttaagaaat gaattaatta aaaataaagc gggagaaaca gtaatccaaa tagaaaaaac
     9421 tgattttaac cctataatag aagaagaaaa caatgtaaac ccaagcgact atcgattggc
     9481 caattggcaa gcttggtggc aattacttgt taaaaattag aaagaaaggt ctcaaccatg
     9541 caatatttta attttgaagg acaaaccgct ttagtcaccg gcgcctctag tggcctaggt
     9601 aagcaattcg ctttattatt agccagcgct ggtgccgatg tcgccattgc tgcccgtcga
     9661 gaggacaaac ttaaagaagt agccgaagaa attgaagcct tgggtagaaa agccctgatt
     9721 ctagtgatgg acgtcaccaa ggaagaagag atcgctgccg gttttgccaa aatcaaaaaa
     9781 gagtggcaaa agctagatat tgtggttaat aatgctggta tttcaagcgc agctcctgct
     9841 gaggaaatgt ccttcgaaga ctggcaaagg gtggtagatg ttaatctgac tggtgttttt
     9901 ttagtggcaa aacatgctgg cgctttaatg aaagaaaaaa aatatggccg aatcattaac
     9961 cttgcttcaa tctacggttt agtgggcaat agtgctttta atgtcgccaa ttatcacgct
    10021 accaagggtg cggttgtcaa tctaacgcgt gctttagcgg ctgaatgggc taaataccag
    10081 atcactgtca acgcgattgg gcccggattt tttgaatcag aaatgactgg tgactttatt
    10141 aacactgatg attttaaaca atatgtcgct atcagttgtc caatgaaaag agttggtaag
    10201 aaaggcgaat tgaatacagc attgcttttc ttagcagata aggctagtag ttacgtcact
    10261 ggtcagacaa ttgcggtgga cggtggttgg accgccatct aaaaaacgcg ccaaaaaagc
    10321 gctatacaaa aa
//
LOCUS       DAOSEW010000016        10228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_5568_length_10228_cov_4.380714, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000016 DAOSEW010000000
VERSION     DAOSEW010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 10228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10228
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5568_length_10228_cov_4.380714"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="PLJ02_01060"
     CDS             <1..537
                     /locus_tag="PLJ02_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOI05042.1"
                     /translation="IDSNGILNVSAKDKNSGKEQKITIQNSTNLSDEEVEKMKSEAEK
                     FAEADKAKRELIETKNKANSVAFEIEKQLKEYGEKLEKADKEKIETDIKALKDLAAKE
                     DVTKEELEKAIDATFASAQKIGEVMQKAQAEEATKGATADKKTESEAKDKTSSAKDEK
                     SKSKEDEKKDAEEGEVVS"
     gene            668..2179
                     /locus_tag="PLJ02_01065"
     CDS             668..2179
                     /locus_tag="PLJ02_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05043.1"
                     /translation="MKKTISRFIFPVLLFTFIIIFFAKGINNYFLGDDWFHLNVTQIN
                     NWREFLNFFNPLENPQQTAFYRPIPNQLFFFIIQSFFGFKPFYYHLPVYLLYLSSIYL
                     FHRLLILLNFSKNSRRFAVLIYSLSATHFTRLFFISAAQEFFMIFFVLLYFLFFLTEK
                     KRFKIKAQLSLIAALLSKDTAVVAPIIIFFIQNQDFLFNKNFRKVKFKINQFFDKNLN
                     FFISVFLVLVYLFIRFFVFNQRMIEDQAYQLNFSIKEAITSVYFYCWWLIGAPELIQD
                     YMPKFYLFLPRFFTDFGRSAYLLIFLALLLLGLLIINFAISYKKLKNIDNLKKIFLAL
                     IFIFFGLLPFIFLPKHRFAIQLALPMLGMAMFLATIFNSHKKIFLTAITLITFLSLNL
                     LSIKMSEKSHYAITRAYISEKTSNYFLNNYPQVESNTVFFITNGQTEGHEIANWGSSR
                     QVAFALWHDNFIQAFYKDKSLQMKFEDLTDPNSLAKLEENNKVIYISAEALID"
     gene            2240..3124
                     /locus_tag="PLJ02_01070"
     CDS             2240..3124
                     /locus_tag="PLJ02_01070"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOI05044.1"
                     /translation="MSAKRDYYEILGLDKSASEADIKSAYRKAALKYHPDRNKAADAE
                     EKFKEINEAYQVLSDKQKRQTYDQFGHAAFDPSSGMGGGAGPFSGFDFSGFGRADNQQ
                     GPFSWSYTRGGDAGGGFDFSDPFEIFEQFFGSGFARARRPRYGINISFMEAVKGTEKE
                     ITLENGKKKKIKIPAGVDNGTRIRFDDFDVSIDVGTHPRFRRDNADVFVDEQIPFSLA
                     ALGGQLTVQTLNNGELKLKIRPGTQSHTLIRLRGEGIKRLRGRGVGDMYVRLIVKVPE
                     KLTREQKRILEEWKKFED"
     gene            3316..4551
                     /gene="nusA"
                     /locus_tag="PLJ02_01075"
     CDS             3316..4551
                     /gene="nusA"
                     /locus_tag="PLJ02_01075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOI05045.1"
                     /translation="MPSTQSIVRTDFAAAVNQIAEERKIEPEDIYEAIRQALVSAYRK
                     QMGELDSSFYYFVELDKETGAAKIFQAPVTKVDEETEEILEWDEAKAEDVTPAGFGRI
                     AAQTAKQVILQKIRLTERDTVIAAYREKIGEVITGQVLRMQGPNVIVDIGRGQGFMPP
                     EEQMRGEFYRLNQRLAVLIKDIEETSRGQQIIVSRSDARLVLKLFEREVPEVATGAVE
                     ITSIAREAGVRTKLIVRSTQSGVDPVGSCVGQRGVRVQKVIEELNNEKVDIIPFAEDN
                     VQLLKAALAPAESLVVEIDEKKKMVTVIAPDDQLSLVIGRGGQNVRLAAKLTGYQITV
                     KSASGAVESAVTGKEEYEIDTFGITDDTREILIQYKLTTLNDLVRFRDKWVDMEEIAD
                     SDRQILLNKVSEYLNGGEE"
     gene            4555..6045
                     /gene="infB"
                     /locus_tag="PLJ02_01080"
     CDS             4555..6045
                     /gene="infB"
                     /locus_tag="PLJ02_01080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOI05046.1"
                     /translation="MALIKRCPVITIMGHVDHGKTTLLDYIRQSHIQASEVGGMTQHI
                     GAYQIMFKNQPITFIDTPGHAAFNKMRQRGAQVTDLVVLVVAANDGVKPQTIESIRHI
                     KDAKVPVMVAINKIDLPDVHPDNVKSQLAEHGILVQEYGGDVEVVEISAKTGQGVEKL
                     LETIVLLTDLMELKADPQAPLEAVVIESTKDKNKGAVARVIVKQGTLKVRQELVVGEI
                     KGKARCLINEKGELSQEVLPGTPIEIVGLAEVPEVGETIREMGANYIGEPPNNISHTG
                     ELEKTLTAGGENVDWDKLDLDYILGDKEKIKLVVKADVKGTLEAILQSLDRDSVEVLR
                     CGVGQITEEDLELASGDKVLLIAFNIKVPNSIKKIAKNMGVKIKEYDVIYHLIEDLQK
                     QMLKLMDSTIDEVVTGEAEILQIFEMKGEKIAGIRVKTGEIKQHDLLHLKRGEEIIAN
                     PVIKLMMHGKDEIQVVKSKHEAGLTFKNKKIDFQVGDLIIAYHQEE"
     gene            6137..6406
                     /gene="rpsO"
                     /locus_tag="PLJ02_01085"
     CDS             6137..6406
                     /gene="rpsO"
                     /locus_tag="PLJ02_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOI05047.1"
                     /translation="MALNKEQKAQIIKDFALFEGDTGSPEVQIALLTASIQELTEHLK
                     THKKDNHSRKGLLKQVSKRRRLLNFLLSRSEERYRKLIDKLGLSK"
     gene            6585..8855
                     /gene="pnp"
                     /locus_tag="PLJ02_01090"
     CDS             6585..8855
                     /gene="pnp"
                     /locus_tag="PLJ02_01090"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF008805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOI05048.1"
                     /translation="MSYPSYENQYHRDVQVGDKTIKFTVGKFSEQVSAAVLAQCGETV
                     VHTTVALGHQVNLGYFPLSVEFEEKLYSSGIIKGSRWVKREGRPTDDAILRARVIDRT
                     MRPLFPEGIKHEVQIINTVLSYDGENEPDMIALLAAGLALQISEIPFDGPVAGARLAL
                     AKNGAKEQFIFNPLNSELEESNLDLIVSASKSSIVMVEAGAKEVDESIIVDALIEAQQ
                     VLSKVCVEIEKIAQEIGKEKVNLVAEPDQKLVALTEKLQAEIKEKYSAEIHQAVIKRA
                     HLEDPGLTELIDQIVAEKAEQATDDDQEITTSLVADIIFQLEKDEARRMILEDGERPD
                     GRKADEIRQIWTEIDVLPRAHGSAMFKRGATQALTVVTLADPSLGQTVEDITGERVRH
                     YMHHYNMPPYASGEAGRIGYPKRREIGHGALAERALLPMIPSQEDFPYAIRVVSEIMS
                     SNGSTSQASVCGSTLSLMAAGVPIARPVSGIAMGLISDGKGKNVILSDIQGLEDHVGD
                     MDFKVAGSEKGITAIQMDIKLKGIDKEILVKALSQAKVGRMHIMQEMLKTIASPRQTI
                     SRFAPKVAQLVIPADKIGTVIGSGGSVIKDIIEKTGAEVNVVEDKEKKQGVINVSSPD
                     QQAIDQALSMIDALVREVEVGDEFQGEVTRVENYGAFVEYLAGREGLLHVSNMSTDYV
                     RDAHEKVKLGDQLKVRIKEIGLDGKVALTALTPEEEAQQQESRGSSRTGSSNRRFDHN
                     NNRSNFHSNRPGNRSR"
     gene            8902..9726
                     /locus_tag="PLJ02_01095"
     CDS             8902..9726
                     /locus_tag="PLJ02_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05049.1"
                     /translation="MQLPAISRQRQLFSILALDDIAELSQLLSLDLSLQDNVLFLSKL
                     LYLLVELSQEASGLVVDPIYSLPLINHKAKNTGILLRLEQHQYPVPHAVPQLFPHFSL
                     LEVKNNYALAKLAIDYQAHEEQALTKKQLLAEIRDYSQSLKIDFLLKLDANFDNQDIL
                     LSTVQEIQSFVDILVLKDINEPLLAATISSELDIPWLLSADPSTTTYDQFKSHYRVAV
                     ENGAKGFYLGNFLWQSLAQFRQENQEFDWPALENHVRTNLRDQIIELNRITEELTR"
     gene            9898..>10228
                     /locus_tag="PLJ02_01100"
     CDS             9898..>10228
                     /locus_tag="PLJ02_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOI05050.1"
                     /translation="MLELQPLPYAYNALEPYIDEETMRIHHDKHHQAYLDKFKLAWQE
                     QPDLKKLSAEQVLWQLEELKEKVDEKTWMALKNHGGGFVHHNVLWEIMSPNGPREPVG
                     ELKEKIKD"
BASE COUNT         3299 a         1587 c         2153 g         3189 t
ORIGIN      
        1 attgatagta atggtatctt aaatgtcagc gctaaagata aaaattctgg caaagaacaa
       61 aaaattacca ttcaaaacag cactaattta agtgacgaag aggtagaaaa aatgaaaagt
      121 gaagctgaga agtttgccga agcggataag gccaaacgcg aattaattga gacaaaaaac
      181 aaagctaata gcgttgcttt tgaaattgaa aaacaactca aagaatatgg cgagaagtta
      241 gaaaaagccg ataaggaaaa gattgaaact gatattaaag ctctgaaaga tttggcggca
      301 aaagaggatg taaccaaaga agagctagaa aaagccattg acgccacttt tgccagtgcg
      361 caaaaaatcg gtgaagtgat gcaaaaagcg caggccgaag aagctactaa aggagccacg
      421 gcagacaaga agactgagtc ggaagcaaaa gataaaacaa gctcggctaa ggatgaaaaa
      481 tctaagagta aggaagatga aaaaaaggat gctgaggaag gcgaagtagt aagttaaaaa
      541 aacttaagat ttttagtcac tagtttagtt attcaaaaaa ttaaactagt gactttttta
      601 ttagcttttt ttattttttg atgttaaatt aattgtaatc agttgattgt tcgattggga
      661 aaatcttgtg aaaaaaacta tttctcgctt tatatttcct gttctgttgt tcacctttat
      721 tattattttc ttcgcaaaag gcattaataa ttactttctt ggtgacgatt ggtttcactt
      781 aaatgttaca caaattaata actggcgaga atttctgaac ttttttaatc cattagaaaa
      841 tcctcagcaa actgcttttt acaggcccat acccaatcaa ttattctttt ttataatcca
      901 atctttcttt ggatttaaac ctttttatta tcatttgccg gtttatttgt tgtatttatc
      961 aagtatttac ctttttcatc gtttgttgat tttgctaaat ttttccaaaa atagcagacg
     1021 gtttgcggtt ctgatttata gtttatcagc cactcacttt actaggctgt tttttatttc
     1081 tgcagctcaa gaatttttta tgattttttt tgttttgctt tacttcttat tttttttaac
     1141 agaaaaaaaa cgttttaaaa tcaaagctca attgtcttta atagcagcgc ttctttcgaa
     1201 agatacagcg gtagttgcac ctattattat tttttttata caaaatcaag attttttatt
     1261 taataaaaac tttagaaaag taaaatttaa aattaatcaa ttttttgata aaaatttgaa
     1321 ttttttcatt agtgtctttt tagtgcttgt ctatttattc attaggtttt ttgtatttaa
     1381 tcaaagaatg attgaagatc aagcttatca attaaatttt tcaatcaaag aagctataac
     1441 cagcgtatat ttttattgtt ggtggttaat aggagctcca gaattgattc aggattatat
     1501 gcccaagttt tatcttttct taccacgttt ttttactgat tttggtagaa gcgcctattt
     1561 attaattttt ttggcactat tgttacttgg tttattaatt atcaatttcg ctatcagtta
     1621 caaaaaactg aaaaatattg ataatttaaa aaaaatattt ttggctctta tttttatttt
     1681 ttttggtctt ttgccattta tatttttacc aaaacacaga tttgcaatcc aattagctct
     1741 gcccatgttg ggtatggcaa tgtttttagc tactattttt aattctcata aaaaaatttt
     1801 tttaactgca ataaccctaa taactttttt aagtctaaat ttgttatcta tcaaaatgag
     1861 tgaaaaaagt cattatgcta ttacacgggc ttatatttct gaaaagacta gtaattattt
     1921 tttaaataat tatcctcaag tggaatctaa taccgttttc tttatcacaa atggtcagac
     1981 agagggtcac gaaattgcca attggggctc aagtcgacaa gtagcctttg ctctttggca
     2041 tgataatttt attcaagcct tttacaaaga taaaagctta caaatgaaat ttgaagattt
     2101 aacagatcct aattcactcg cgaaattaga agaaaataat aaagttattt atatttcagc
     2161 tgaagcattg attgattagc aatcaccatt tttgattgct taattttttt atttttttag
     2221 tgtacaattg atcagtttta tgtcagccaa acgcgattat tacgaaattt taggtctaga
     2281 taaatctgct agtgaagcag atatcaaatc tgcttatcgt aaggctgcgc tcaagtatca
     2341 tcccgatcgt aacaaagcgg ctgatgctga agaaaaattt aaagaaatca atgaggctta
     2401 tcaggtactt tctgataagc aaaaacggca aacctatgac caatttggtc atgctgcctt
     2461 cgatccctct agcgggatgg gtggtggagc tgggcctttt tctggttttg atttttctgg
     2521 ttttggtcga gctgataatc aacaagggcc atttagttgg tcctacacta gagggggtga
     2581 cgccggagga ggttttgatt tttccgatcc tttcgaaatt tttgaacaat tttttggtag
     2641 tggtttcgct cgcgctcgtc gccctcgtta tggaatcaac attagtttca tggaagctgt
     2701 taagggaacc gagaaagaaa ttaccctaga aaatggtaaa aagaagaaaa ttaaaattcc
     2761 cgctggtgtg gataatggca ctcggattcg ttttgatgat tttgatgtca gtattgatgt
     2821 tggaactcat cctcgcttta ggcgggataa tgctgatgtt tttgttgatg aacaaattcc
     2881 attcagcttg gcggctttag gtggtcaatt aactgttcaa acgctgaata acggtgaatt
     2941 aaagctcaaa attcgtcctg gtactcaatc acataccctg attagacttc gtggtgaggg
     3001 catcaaacgc ttaaggggtc gtggtgtagg tgatatgtat gtgcgtctga ttgttaaagt
     3061 tccagaaaaa ctcactcgag agcagaaaag aattttggaa gagtggaaaa aattcgaaga
     3121 ttagtctaac tagtctgatt ttgcactctt ttcaagcaaa tcttcattac tgcgctgaat
     3181 ttacaaaaag ctcaatttct gttataataa cagtttgaaa ttaaggtggt ttgtaaagga
     3241 ctgatcttga cgataagtcc ttttttatta atcagctaat ttctcaagtt tagaattatt
     3301 taataaggga aaaatatgcc aagtactcag tcaattgttc gaacagattt tgcggcagca
     3361 gtcaatcaaa ttgccgaaga gcgcaagatt gaaccagagg atatttatga agctattcgt
     3421 caagctttgg tatctgccta tcgcaaacaa atgggcgagc tagatagctc tttctattat
     3481 tttgttgaac tagataaaga gactggtgca gccaagattt ttcaggctcc agtgactaaa
     3541 gtcgatgaag aaactgaaga aattcttgaa tgggatgaag ctaaggccga agatgtaacg
     3601 ccggctggtt ttggtcgaat agctgctcaa actgctaaac aagtgattct acaaaaaatt
     3661 cgtctgaccg agcgcgatac ggtaattgct gcctaccgcg aaaagattgg tgaagtgatt
     3721 actgggcaag tactaagaat gcaaggtccc aacgtgattg ttgatattgg tcgtggccag
     3781 ggctttatgc ccccagaaga gcaaatgcgt ggtgaatttt accgcctgaa tcaacgtctg
     3841 gccgtcttga ttaaagacat tgaagaaacc agtcgtggtc agcaaatcat tgtttcacga
     3901 tctgatgcgc gcttagtgtt aaagttattt gaaagagaag tgcctgaagt cgccactggt
     3961 gctgtagaaa tcacttctat tgctcgagag gctggcgtgc gtactaaatt aattgttcgt
     4021 tctacgcaaa gtggcgtaga tcctgttggt tcctgtgtgg gtcagcgtgg agtccgagtg
     4081 cagaaagtga tagaagaact gaataatgaa aaagtcgata tcattccttt tgccgaagac
     4141 aacgtacaat tattaaaagc ggctttggct ccagcagaaa gcttggtggt ggaaattgat
     4201 gaaaagaaaa aaatggtgac tgtgattgct cccgatgatc aattgtcttt ggttattggt
     4261 cgaggtggac aaaatgtgcg cctagcggcc aagcttactg gttatcaaat tactgttaag
     4321 tcagccagtg gtgcagttga atcagcagtg actggtaaag aggaatatga aattgatact
     4381 tttgggatca ccgatgatac tagagaaata ttaattcaat ataaattaac caccttaaat
     4441 gatttagtcc gctttcggga taaatgggta gatatggaag aaattgctga tagcgatcga
     4501 cagattttat taaataaggt tagtgaatat ttaaatggag gtgaagaata aactatggct
     4561 ttgattaaac gttgtccagt tatcactatc atgggtcatg tcgatcacgg caagaccact
     4621 ttgcttgatt atattcgtca gagtcatatt caggctagtg aagtgggtgg gatgacccaa
     4681 cacattgggg cttaccaaat catgtttaaa aatcagccaa ttacttttat cgatacgcca
     4741 ggacacgcag ccttcaataa aatgcgtcag cgtggagctc aggttactga tttagtggta
     4801 ctcgttgtgg ctgccaatga tggggttaaa ccgcaaacca tcgagtcgat tcgccacatt
     4861 aaagacgcta aagtgccagt aatggtagct atcaataaaa ttgatttacc agatgttcat
     4921 ccagacaatg tgaagagtca gttggccgaa catggaattt tagttcaaga atatggtggc
     4981 gacgtggagg ttgtagaaat ttccgctaaa actggtcaag gagtagaaaa gttgctagaa
     5041 actattgtgc ttttaaccga tttaatggag ttaaaagctg acccgcaggc tcctctggaa
     5101 gcggtggtga ttgaatcaac taaagacaaa aataagggtg ctgtagccag agtgattgtt
     5161 aaacaaggta ctttaaaagt ccgtcaagaa ttagtggtag gtgaaataaa aggtaaagct
     5221 cgttgtttaa tcaatgaaaa aggtgaacta tcccaagaag tcttaccagg tactcctatc
     5281 gaaattgttg gattagctga agtacctgag gttggtgaaa ctattagaga aatgggagct
     5341 aattatattg gtgagccacc aaacaatatc agtcatactg gagaattgga aaaaactctc
     5401 actgctggtg gggaaaatgt tgattgggat aaattagatt tagactatat tttgggtgac
     5461 aaagaaaaaa ttaaactggt cgttaaagct gatgttaaag ggacgctgga agcaatttta
     5521 caaagtttag atcgtgatag tgttgaagtt ttgcgttgtg gagttggtca aattactgag
     5581 gaagatctag aactagctag tggcgataaa gttttattaa ttgctttcaa tattaaagta
     5641 cctaactcga ttaagaaaat cgccaagaat atgggagtta aaatcaaaga atatgatgtc
     5701 atttatcact taattgaaga tttacagaaa caaatgctca agttgatgga ttcgacgatt
     5761 gatgaggtag tcactggtga agctgaaatt ttacaaatat ttgaaatgaa aggtgaaaaa
     5821 attgctggta ttcgagtaaa aactggtgag attaaacaac atgatttact acatctcaaa
     5881 cgtggtgaag aaattatagc taatccagtc attaaactga tgatgcatgg caaagatgaa
     5941 atccaagtag ttaagtctaa gcatgaagcc ggtttgactt ttaaaaataa aaaaatagat
     6001 tttcaagttg gtgatttaat tatcgcttat catcaagaag aatagctgtg atataatatc
     6061 caggttaaaa aataataatg cttactttgc caattaggtt aatacttaat ggttaaaggc
     6121 aaaatcgaaa ggacttatgg cattaaataa agagcaaaaa gctcaaatta tcaaagactt
     6181 tgctttattt gagggtgata ctggttctcc agaagtgcaa attgctttgt taactgcttc
     6241 cattcaagag ttaaccgaac atctaaaaac ccacaagaaa gataatcatt cccgcaaggg
     6301 tttgttaaaa caagtttcta agcgccgtcg tttattaaat ttccttttga gtcggagcga
     6361 agaacgttat cgaaaattaa ttgacaaatt aggtctgagt aaataactcc agcgagatat
     6421 ttgctaaagt ttgctataat gttctctgtt aataattaag aaagcaaaat ttggggtgtt
     6481 aggtgaggat tcaaaaaagt tgataagttt tatcaatttt tcagaatact catatttccc
     6541 cagttttgtt tgtagctctt atagagcaaa gaagaaagaa ggatatgtct tatccaagtt
     6601 atgaaaacca atatcatcgt gatgtgcaag tcggtgataa aactattaaa tttaccgttg
     6661 gtaaatttag tgaacaagta agtgctgcgg tacttgctca atgtggagaa acagtagtgc
     6721 acactactgt tgccctcggt caccaagtga atcttggtta ctttcctttg tcagtagaat
     6781 ttgaagaaaa actttattct agtggcatta ttaagggttc gcgttgggta aaaagagaag
     6841 gtcgaccgac cgatgatgcg attctcagag cgagagttat tgatcggact atgcgtcctc
     6901 tatttcccga aggtattaaa catgaagtac aaattatcaa tacggtgttg tcatatgatg
     6961 gtgaaaatga accagacatg attgccctgc tggcagccgg tttagccctg caaatcagcg
     7021 aaattccttt tgatgggcca gtagctgggg ccaggcttgc tttggctaaa aatggtgcca
     7081 aagaacaatt catttttaat ccattaaata gtgaattaga agaaagtaat ttagatttaa
     7141 ttgtttccgc ttccaaatct tccattgtta tggtggaagc aggagcaaaa gaggttgatg
     7201 aatcaatcat agttgatgca ttaattgaag cccaacaagt tttatcaaaa gtttgtgtgg
     7261 aaattgaaaa aattgctcaa gaaattggca aagaaaaagt caatttagtt gcagagccag
     7321 atcaaaagtt agtagctttg acggaaaaac tgcaagctga aattaaggaa aaatacagtg
     7381 cagaaattca ccaggcggtt attaaaagag ctcatttaga agatcctggt ttaactgagt
     7441 tgattgatca aattgtggcc gaaaaagcag agcaagccac agatgacgat caggaaatta
     7501 ccactagcct ggtagccgac attatttttc aattagaaaa agatgaagca agaagaatga
     7561 tccttgaaga tggagaacgt ccagatggcc gaaaagctga tgagattaga cagatttgga
     7621 ctgaaattga tgttttaccc agagctcatg ggagtgctat gtttaagcgc ggcgctactc
     7681 aagctttgac tgtagtaact ttagctgatc cttcgctagg tcaaactgtt gaggatatta
     7741 ctggtgaaag agtgcgccat tatatgcacc actataatat gcctccttat gcttctggag
     7801 aagcaggtcg aattggttat ccaaagagac gagaaattgg tcatggcgct ttggcagaac
     7861 gagcgctact gcctatgatt ccttctcaag aagattttcc ttacgctatt cgggtagtta
     7921 gtgaaattat gagttctaat ggttcaactt cacaggcttc ggtctgtggg tctaccttat
     7981 ctttgatggc ggctggggtg cctattgctc gaccagtttc tgggattgcg atgggtttaa
     8041 tatctgatgg taagggaaag aatgtgattt tgtctgacat tcagggtcta gaggatcacg
     8101 ttggtgatat ggacttcaaa gtggcaggta gtgaaaaagg gatcactgct atacaaatgg
     8161 atattaaatt aaaagggatt gataaagaaa ttttggttaa ggctctttct caggctaaag
     8221 taggtcggat gcatattatg caagaaatgc tcaagacgat tgccagcccc cgccaaacaa
     8281 ttagtcgatt tgctccaaaa gtagctcaat tagtgattcc agctgacaaa attggtaccg
     8341 ttattggctc aggtggcagt gttatcaaag atatcatcga aaaaactgga gctgaagtta
     8401 atgtggttga agataaggag aaaaagcagg gagtgatcaa tgtttcttct ccagaccaac
     8461 aggcgattga tcaagctttg agtatgatcg atgctttagt tagagaagtc gaagtcggtg
     8521 acgagtttca aggagaggtg acgagagtag aaaattatgg tgcctttgtt gaatatttgg
     8581 ctggccgaga aggcttattg catgtttcta atatgtcaac tgattacgtt agagatgctc
     8641 atgaaaaagt aaaactaggt gatcagctga aagtgagaat caaagaaatt ggtcttgatg
     8701 gtaaggttgc tctcacggct ctaactcctg aagaggaagc tcagcaacaa gaaagtcgag
     8761 gtagttcccg aactggtagc agcaatcgac gttttgatca caacaataac cgatctaatt
     8821 ttcactcaaa ccgtccaggt aacagaagtc gctagtcaag aactgctcac ttagccagct
     8881 attttttgtt atactaagga gatgcaactg cccgcaatta gtcgtcaacg tcaactgttt
     8941 agtattttgg ctttagatga tattgctgaa ttgagccagc ttctttcatt agatttgagt
     9001 ttacaggata atgtcctttt tttaagcaag ttgttgtatc tattagtgga attatctcaa
     9061 gaagcgagtg gtttggtagt tgatccaatt tatagtttgc ctttgatcaa ccataaagca
     9121 aaaaatactg gtattttgct tcgtttagag caacatcagt acccagttcc tcacgctgta
     9181 cctcagcttt ttcctcattt ttctttacta gaagtgaaaa ataattacgc tttagctaaa
     9241 cttgccattg actatcaggc tcacgaagaa caggctctga ctaagaagca attattagct
     9301 gaaattaggg attatagtca aagcttgaaa atcgattttt tgctcaaatt agatgctaat
     9361 tttgataatc aggatatttt gttaagcact gttcaagaaa ttcaatcttt tgtggatatt
     9421 ttagttctaa aagatattaa tgagccatta ttggcagcga ccattagtag tgaactagat
     9481 ataccatggt tgttgtctgc tgatccatca accacaacct atgatcaatt taagagtcat
     9541 taccgcgtgg cagtggaaaa tggagccaaa ggtttttatt taggaaattt tttatggcaa
     9601 agtttggctc aatttagaca agagaatcaa gagtttgatt ggccggcatt ggagaatcac
     9661 gttcggacca atcttcgtga tcagattatt gaattgaatc ggattaccga agagttgact
     9721 agataaaatt ggcagagtca gccatgtaga attgcaaagt tgaccataaa taattttggg
     9781 taaaattgtt gtctgtatat ttatttatca ttgattaatc aatcttttta attattgatt
     9841 ttaggcatta tttttgctat gatctttatg taattattaa tcaaaaaaaa ggacaagatg
     9901 ttagaactcc aaccccttcc ttatgcttat aatgcgcttg aaccctatat tgatgaagaa
     9961 accatgagaa ttcatcatga taaacatcat caagcctatt tggataaatt caaattagct
    10021 tggcaagaac aacctgattt gaaaaaatta agcgctgaac aggttttgtg gcaactagaa
    10081 gaacttaaag aaaaagttga cgaaaagacc tggatggctt tgaagaatca tggtggtggt
    10141 tttgttcacc acaatgttct ttgggaaatt atgtcaccta atgggcctcg agaacccgtt
    10201 ggcgagctca aagaaaaaat caaagatc
//
LOCUS       DAOSEW010000017         9694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_5978_length_9694_cov_4.195145, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000017 DAOSEW010000000
VERSION     DAOSEW010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 9694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9694
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5978_length_9694_cov_4.195145"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="PLJ02_01105"
     CDS             <1..736
                     /locus_tag="PLJ02_01105"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOI05051.1"
                     /translation="RDDNITQAGLAIGNASNVDHFSDILALLILQNGGDPTDPTSQNV
                     VDALTFYTNFIRQDKVWSNNLPNSTMAFARGEVAMIFAPSWRALEIKQINPELDFAIA
                     PVPQLSNEEKVTWASFWAEGVNKNSSQSEAAWKLLDYLSSPEVLKKFFNSASLVRTFG
                     EIYPRTDMANELSEDELLGAYVADAPFAQSFPMTSFTHDNGLNDQLIQYYADAINAVL
                     AGTQPDKALQEISSGISQVVQKYGLN"
     gene            complement(733..1767)
                     /gene="yvcK"
                     /locus_tag="PLJ02_01110"
     CDS             complement(733..1767)
                     /gene="yvcK"
                     /locus_tag="PLJ02_01110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HOI05052.1"
                     /translation="MPKKKIVVIGGGTGTAVVLSGLKQYPDLEITAIVVVTDNGGSTG
                     RLRDEFGFLPAGDLRQCLAALATGENESLIRDILLYRFSKQSSLAGHNLGNLILTALE
                     DLARERKDSPARAIEIASQVFETAGRVYPISEHPADLVIDYQDKQIIGEKNLDNPKLG
                     GQKIKKISLKEKQRIYHRAAQAIEEADLIILGPGDLYASLLANTLVDGFAEAMHNNQK
                     RGGKFVYVLNLMTHFSQTHQMTAFDHLSEVNKYCQRQPDYVLINSEKISPKILEHYAS
                     QNEWPVIDDLNQAKKNQQSVIDWSKLNVKRHHLLSTVLVSHEPGHSHSLLRHDKNKLA
                     QSLIKILKNS"
     gene            1810..2289
                     /locus_tag="PLJ02_01115"
     CDS             1810..2289
                     /locus_tag="PLJ02_01115"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HOI05053.1"
                     /translation="MNLPEELSNILRIREQSHSQLTLVTGVFDVLHQEHRRFLQAAKQ
                     LSEILVVGLESDQRVREMKGEDRPIHSISERQANLQAWGLADVIFTLPDDFGQATVRR
                     QILQTIKPDYLAVSSHTPFLAVKRAELKAIGGQVKIVHQFNPQVSSTKLIRKTKKLN"
     assembly_gap    2301..2400
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2446..3816
                     /gene="radA"
                     /locus_tag="PLJ02_01120"
     CDS             2446..3816
                     /gene="radA"
                     /locus_tag="PLJ02_01120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOI05054.1"
                     /translation="MAKVLKTQFVCQNCGAVHPKWQGQCNECHQWNTLVEEMVSQKNE
                     KKSSINLSKTSLNKKMVNFSQVERLSGAKQRLLTEIGELDRVLGGGLVTGMTILIGGE
                     PGIGKSTLLTQMVLAILAKNINALKIFYVCGEESPNQISLRIERMLKNSKTKNLREKL
                     NQSLQFVTSSDVDEITALMASNKPNLVIVDSIQSITTSDLTGASGSVGQIRECSSRFI
                     DQAKVQGTPIFLVGHITKDGNIAGPKILEHMVDTVLLMEGERSGQLRILRSLKNRFGA
                     SDEVGIFQAVDWGLNEVTNPSELFLENQKQPVSGAATVCVMEGTRPLLTEVQALVVSS
                     QLAMPRRVGRGIDISRIQVLTAVLQKHARLPLSNYDVFVSAAGGFKLSEPAVDLGLAV
                     AIASSLSGKKIPPKTVFIGEIGLLGEIRQVAYFDRRVKEAKRLGFTKIISAQSHRHIN
                     QVIKEL"
     gene            4676..6112
                     /gene="rho"
                     /locus_tag="PLJ02_01125"
     CDS             4676..6112
                     /gene="rho"
                     /locus_tag="PLJ02_01125"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOI05055.1"
                     /translation="MQNSQDNSVSAIANSVDSKLKSSKSSQEENQSKSTTRVIKRRVG
                     GKSNAAKAETDQASNSSENMNGRSSERSSEHSNGHSSEHSNGHPSYNDSNNLDNNENK
                     EQEIIVSGILDNSKEGHGVLRPEFSESEQDVYISSSQIRRLRLKSGDLVTGPARQPKD
                     NERYWGLLRVDSINGRSVEEHRQLNRKNFLSLTPVFPDEHIKLEIGAEPLSLRLIDLI
                     APIGKGQRSLIVSPPKAGKTTILKDVALGVATNNPDIHLMAVLVGERPEEVTDIRRHI
                     KKITNGKGEVAASNFDEPAQNQCRVAELALERAKRLVEEGRDVLILLDSITRLARAYN
                     LAMPTSGRTLSGGFDPAALFPPKKFFGAARNFEVPGSLTIIGTALVDTGSRMDDLIYE
                     EFKGTGNQELHLDRALAERRIYPAIDLQRSGTRRDDLLIDPVVYQSIITLHRMLDMLG
                     KDERTATVIEKLKRTKSNKEFLASLKNG"
     gene            6119..7306
                     /locus_tag="PLJ02_01130"
     CDS             6119..7306
                     /locus_tag="PLJ02_01130"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOI05056.1"
                     /translation="MPEKNQVKKLSMWNRDLYHQRQQGKLQFLIKDLQSFLRFLRSYG
                     KIRLYVIFDFFEKLKDALVSVLHQKRGKYTRPFLHFGTIALSFFMVIFGPRILNSNDE
                     TMDSQLVLATPILAVSTATAPDFYTYQAEEVRQYRGGEIITHVVEEGETISDIAERYG
                     LRKETILWANNLTEKSTIKPGQSLEILPVDGVRHKVVRGDTIYSIGKKYGLTGSQVQM
                     IVDYPFNEFLNDETFDLVAGQYLMVPEGRPPTAAVSPIATSRSNFGVLTPDAGTVSAT
                     GSFIWPASGGISQGYSFYHKAIDISNGAGGAILAADAGVVAVAGWVDGYGYGNRVMID
                     HGNGFVTLYAHLSVVQVRVGQRVGRGDVIGQMGSTGRSTGTHLHFEIRQGGALQNPFN
                     FLR"
     gene            7317..8573
                     /gene="obgE"
                     /locus_tag="PLJ02_01135"
     CDS             7317..8573
                     /gene="obgE"
                     /locus_tag="PLJ02_01135"
                     /inference="COORDINATES: protein motif:HMM:NF008956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOI05057.1"
                     /translation="MIDLVTLKLAAGNGGQGKISFRREKYVPKGGPDGGDGGDGGSII
                     IRATKALSTLRPYAGVKEIVAESGQPGAKRKQHGQKGEDRIIEVPVGTTVTLLAENHT
                     SAYRRQRYGFVNQDGSQRLLRSADFQAGQVPPGTNIKRQRYFIDYEVSDSNLVLEPDE
                     LEALPQPEELCQLMEDGEELVLCQGGFGGRGNSQFKSSQKTTPREAEHGTFGEQKLVK
                     LELRLLADLALVGFPNAGKSTFLSKVTAANPKIANYPFTTLEPNLGIMQLQKGAQEIV
                     VADIPGLIEGANQGKGLGHDFLRHIENCQALMYVLFLEESVIFDQALSAKDKSQLLWE
                     QYQNLNNELKQHNPQLLEKPYLVTLNKADLYSQAEIEQFQADFAKHKITLLVFSAITG
                     EGLSAVVKNIEKLFLITKTSLSSKNY"
     gene            complement(8567..>9694)
                     /locus_tag="PLJ02_01140"
     CDS             complement(8567..>9694)
                     /locus_tag="PLJ02_01140"
                     /inference="COORDINATES: protein motif:HMM:NF024823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOI05058.1"
                     /translation="NTMMLTLLAIALSFYLAANLDSYKIKKFSFNNLFDPFNLQPENK
                     ANRYTFATISVLLMAGLIFLTYQIGLHYAAHAAAYQASKAASEEKFNEVFAKQEQSRN
                     LASRSADWRRIYASSALELAVALSNKTDITPVEQEQVIELINVAITEAKAATILQPRS
                     YQNWLTLANIYLQLLDVTEDARQLAFESLSQAALTNPNDPFMRINLGQFMMRLEQWEQ
                     ASNFFIQAIERKPDLPVAYYNLAQAYIKNQQILEAENALQQALLLIDQETNLEDYNTV
                     KTLLESISEEADQLRAQAEAQQAQQAQQAQQGQQTPQEELTTPAAPAKQVEPDITNQQ
                     TGLEPATATESGLSDLLNEEETSNLLRESSLVPEEETIDNQ"
BASE COUNT         2831 a         1722 c         2101 g         2940 t
ORIGIN      
        1 gcgcgatgat aatattactc aagcgggctt ggctattggc aatgctagta atgttgatca
       61 tttttccgat attttggcct tactgatttt gcaaaatggt ggtgatccga ctgatccaac
      121 tagtcaaaat gtagtggatg cgctgacttt ttacacgaat ttcattaggc aggacaaagt
      181 ctggtcaaac aatttaccga attcaaccat ggcttttgcc agaggagagg tggctatgat
      241 ttttgcccca tcctggcggg ctttggaaat caaacaaatt aatccagaac tagattttgc
      301 tattgctccg gttcctcaat tatctaatga ggaaaaagtt acttgggcca gcttttgggc
      361 tgaaggggtt aataaaaata gtagtcagtc agaagcagct tggaaattgc ttgattattt
      421 atcttctcca gaggtattga agaaattttt taatagtgcg agtttagtga gaacttttgg
      481 cgaaatttat ccccgaactg atatggcaaa tgagcttagt gaagatgaac ttttgggcgc
      541 ttatgtagca gatgcgccat ttgctcagtc atttcccatg actagtttca cccatgataa
      601 tgggttaaat gatcagctaa tccaatatta tgctgatgcc attaacgcag ttttggctgg
      661 aactcaacca gacaaggctt tgcaggaaat tagttcagga atttctcaag tagtgcagaa
      721 atacgggtta aattaactat ttttgagtat tttaattaat gattgggcta gtttattttt
      781 gtcatgtctc agtagactat ggctatgacc aggttcgtga ctgactagga cggtagagag
      841 tagatgatga cgtttaacgt tcaatttaga ccaatcaatg actgattgct ggtttttttt
      901 ggcttgattt aaatcgtcga tcactggcca ttcgttttga ctagcatagt gttcaagtat
      961 ttttggagaa attttttcac tatttatcaa aacataatca ggttgtcgtt gacaatattt
     1021 attcacttcg cttaaatgat caaatgctgt catttggtga gtttgtgaaa aatgagtcat
     1081 caaatttaaa acgtagacaa attttccacc gcgtttttga ttattgtgca ttgcttcagc
     1141 aaagccatcc accaaagtat tagctaacaa actggcgtag aggtctcctg gtcctaaaat
     1201 aatcaggtca gcttcttcaa tggcttgggc agctcgatga tagattcttt gtttttcctt
     1261 gaggctgatt tttttaattt tttgcccacc gagtttggga ttgtctaaat ttttttcacc
     1321 aataatttgt ttatcttgat aatcaataac cagatctgct ggatgttcgc taatcgggta
     1381 aactctacca gctgtttcaa aaacttgact agcgatttca atggctcgcg ctggactatc
     1441 ttttctttct cgggctaaat cctcaagagc agttaaaatt aagttgccca aattatgacc
     1501 agctagactg gattgtttgc taaatcgata gagtaaaata tcgcgaatta atgattcatt
     1561 ctcgcctgtg gccaaggcgg cgaggcattg gcgcagatca ccagcaggta aaaagccgaa
     1621 ttcatcacgc agtcgaccag tggaaccgcc attatcagtt actacaacaa tagcggttat
     1681 ttctaaatct ggatattgtt tcaaaccact taacaccact gctgtcccag tacccccacc
     1741 aatgactaca attttcttct ttggcatttt gcctttttat tttaacagat cattggttag
     1801 aataggctta tgaacttacc agaagagtta agcaatattt tgcgaatccg cgagcagagt
     1861 cacagccaat taactctagt cactggagta tttgatgttt tacatcaaga acatcgtcga
     1921 tttttgcaag cggccaagca actgagcgaa attttggtgg tgggtttgga aagtgatcaa
     1981 cgtgtgcgag aaatgaaagg tgaggatagg ccaattcatt ctatcagcga gcgtcaagct
     2041 aatttgcaag cttggggctt ggctgatgtt atttttactt taccagatga ttttggtcaa
     2101 gctacggtac ggcgacaaat tttgcagaca ataaaaccag attatttagc agtttctagc
     2161 cacactccat ttttggctgt caagagagcc gagctgaaag ctattggtgg tcaagtgaaa
     2221 attgttcatc aatttaatcc acaagtttca agcactaaac tgattagaaa aacgaaaaaa
     2281 ctaaactgat tagaaaaacg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 aaactgatta gaaaaacgaa aaaacaaaag gagtaacaat caataatggc aaaagtacta
     2461 aaaacccaat ttgtttgtca aaactgtggc gctgttcatc ccaaatggca ggggcagtgt
     2521 aatgagtgtc accagtggaa tactttggta gaagaaatgg ttagtcaaaa aaatgaaaaa
     2581 aagtcttcaa tcaatttgtc taaaacatct ttaaacaaaa aaatggtaaa tttttctcaa
     2641 gtcgagcgtc tcagcggtgc aaaacagcgg cttttgactg agattggaga gttggatagg
     2701 gttttgggcg ggggcttggt aaccggcatg accattttga ttggcggtga accgggaatt
     2761 ggcaaatcca ctttgttaac ccaaatggtt ttagccatat tagccaaaaa tatcaatgca
     2821 ttgaagattt tttatgtttg tggtgaagaa agtcccaacc aaattagtct acgtattgaa
     2881 cggatgctca aaaacagcaa aactaaaaat ttacgtgaga agctcaatca atctctgcag
     2941 tttgttacca gtagcgatgt ggatgaaatc accgcactca tggcaagtaa taaacccaat
     3001 ttagtgattg ttgatagtat tcagtcaatc actacgagtg acttgacggg ggcaagtggt
     3061 agcgttgggc aaattcgaga gtgcagttca cgctttattg atcaagccaa agtacaaggg
     3121 acaccaatct ttttagttgg ccatattact aaagacggta acatcgctgg accgaaaatt
     3181 ttagaacaca tggtcgatac ggtcttactg atggaaggtg agcgctctgg ccaattacgg
     3241 atcttgcgtt cgctaaaaaa tcgttttgga gcaagtgatg aagtgggcat ttttcaggcg
     3301 gttgattggg gactgaacga ggtgaccaat cctagtgagt tatttctgga aaatcaaaaa
     3361 cagccagtca gcggggcagc tactgtatgt gtgatggaag gcactcggcc gctattaacc
     3421 gaggttcagg ctttagttgt ctccagtcag ctagcgatgc cgagacgagt ggggcgaggg
     3481 atcgatatta gtcggattca agttttgact gcagttttgc aaaaacacgc tcgtttacca
     3541 ctaagtaatt atgatgtatt tgtgtcagca gcgggaggtt ttaaattaag tgagccagca
     3601 gtggatttgg gtttggcagt ggccattgct agttcactct ctggtaaaaa aattcccccc
     3661 aaaacggttt ttattgggga gattggttta ctaggggaga ttagacaagt ggcttacttt
     3721 gaccgtcgag tcaaggaggc taagcgcttg ggtttcacta aaattatttc agcccagagc
     3781 catcgccaca tcaatcaggt catcaaggag ctttagtcga cctagtgttc gaattaattc
     3841 ttcaattttg ttattttttt cttatttttt catttttttc ttattttcta cagttttccc
     3901 aagtttttcc caaaattgta aatattgtaa ttaatgtgga aaactttata ttttaatctg
     3961 gattttgact aaataaatta atataggaca ttaaaaaaaa cctgttttta aaattatagg
     4021 atgatattaa tgcttgtcaa aattataaaa actgctaaaa ttaatttagt tttggtactt
     4081 taatagcaac aaaggatatt tagccttttt tgggtcaaca ctcaagaggg gataaaaaaa
     4141 tccatcccgt tcaagtcttg gtttttaggc agtcaagaac ctaaacagaa tggattgttg
     4201 agaacattgt taccgctgat tgttcacgaa taacaatgta atcctgtcgt tgttgaaata
     4261 aatcaacaac tgatcgctta ttaagctaac acttttcgtc atttttgaca attctagctt
     4321 aaaatataga agtatatcaa aaagtatcag agccgagtag gactgccaac acaagaactt
     4381 tttgtggaat ctcggtcgtt gggcaggcta ccgagactcc gcagaaagtt cttttttcat
     4441 tccagagaca atcaccagaa taaacccttt taccattgaa ataattgcca gcgccagatt
     4501 tttatcattg caataatcgt cacagaaagc cctttttatc attgatcttg atttttctgt
     4561 atttttttga cataatccag ttgattttca gtttttattt ctaaattaat cgtgatgccc
     4621 catttttctt atcttttttt ctagccatga cacttattta ttagaaagta attttatgca
     4681 aaacagtcaa gataattctg tatccgctat tgccaattca gttgacagca agctcaagag
     4741 tagtaaaagt tctcaagaag agaatcagag taaatccact acgcgggtga ttaaacgccg
     4801 agtaggtggt aaaagtaacg ctgccaaagc tgaaactgat caagctagta attcaagtga
     4861 gaatatgaac ggtcgctcca gtgaacgctc aagtgagcat tccaacgggc actcaagtga
     4921 gcactccaac gggcacccta gctacaatga tagtaataat ttggataaca atgagaataa
     4981 ggaacaggaa attattgttt caggtatttt ggataattct aaagaaggtc atggagtact
     5041 gcgaccagag ttttcagaat cagaacaaga tgtttatatc tctagttcac aaattcgtcg
     5101 tttacgatta aagtctggtg acttggtcac tggcccagct cgtcaaccta aagacaacga
     5161 acgatattgg ggcttattgc gcgttgacag tattaacggt cggtctgtag aggaacatcg
     5221 tcaacttaat cgtaagaatt ttttaagctt aaccccagtt tttccagatg aacatattaa
     5281 attagagatt ggtgcagaac cattatcttt acgtttgatt gatttaattg cgccaattgg
     5341 taaaggtcaa cgtagtttaa tagtctctcc gccaaaagct ggcaaaacca ctattcttaa
     5401 agatgtggct ttgggagtag ccaccaacaa ccccgacatt catttaatgg cagttttggt
     5461 tggtgaacgc ccagaagagg taaccgatat ccgtcgccat attaaaaaaa tcactaacgg
     5521 gaaaggtgaa gtagcggcga gcaactttga cgaaccggca cagaatcaat gccgagtagc
     5581 agaattagct cttgagagag ctaaacgcct agttgaagaa ggtcgcgacg tgttaatttt
     5641 actcgattct attactcgtt tagcgcgagc ttataacttg gccatgccaa cttcaggtag
     5701 aaccctatct ggtggttttg atcctgccgc cctctttccg cctaaaaagt tttttggggc
     5761 agcgcgaaat tttgaagtac caggttcact gacgattatt ggtactgctt tggtggatac
     5821 cggttcgcga atggatgatc tgatttatga agagtttaag ggtactggta atcaagaatt
     5881 acatttagat cgtgctctgg ctgagagaag aatctatccc gctattgatt tgcagagatc
     5941 tggtacgcgc cgtgatgatc tattaattga tcccgttgtt tatcagtcta taattacttt
     6001 acatcgtatg ttagatatgc ttggtaaaga tgagagaaca gcgacggtga ttgaaaagct
     6061 gaaaagaact aaatctaaca aggaattttt agcctcgctc aaaaatggct aaagattttt
     6121 gccagagaaa aaccaagtta aaaagttaag catgtggaac agagatttat atcatcagcg
     6181 ccaacaggga aaattgcaat ttttgattaa agatttgcaa tcttttttac gatttttgcg
     6241 atcttatggc aagatccgcc tgtatgtaat ttttgatttt tttgaaaaac ttaaagatgc
     6301 tctggtgagt gtcttacacc aaaaacgtgg taaatatact cgaccttttc tgcatttcgg
     6361 gaccattgct ttgagttttt ttatggttat ttttgggcca cgtatattaa attccaatga
     6421 tgagacaatg gatagtcagt tggttttagc taccccaatt ctagcagtta gtaccgctac
     6481 tgctcctgac ttttacactt atcaggcaga agaagttcgt caatatcgag gcggagagat
     6541 tattactcac gtggttgaag aaggtgaaac gatttctgac atcgctgagc gttatggttt
     6601 gcgcaaggaa accattttgt gggccaataa tttgactgaa aaaagcacca ttaaaccagg
     6661 gcaaagccta gaaattttgc cggtcgatgg agtgagacac aaagtggtga ggggagacac
     6721 catttattca attggaaaaa aatatggtct gactggttct caagtacaga tgattgttga
     6781 ctatcctttt aatgaatttt tgaatgatga aacttttgat ttagtagccg gacaatatct
     6841 aatggtgcct gaggggagac ccccaacagc tgctgtttct ccaattgcta cctcccgcag
     6901 caattttggg gtactgactc cagatgctgg gacggtttct gctactggta gctttatttg
     6961 gccagccagt ggggggatta gtcaaggtta tagtttctat cataaggcga ttgacatctc
     7021 taatggtgct ggtggggcaa ttttggccgc agatgctgga gtagttgctg tcgctggctg
     7081 ggtagatggt tatggctatg gtaatcgagt gatgattgat cacggtaatg gttttgttac
     7141 gctctatgct cacttgagcg tggtccaagt gagagttggc caacgagtgg gtcgaggcga
     7201 tgtcatcggc caaatgggca gtactggccg aagtaccggt acacatttac actttgaaat
     7261 ccgccagggt ggtgccttac aaaatccatt taatttttta agataaggta attttcatga
     7321 tagatcttgt taccctcaag ttagctgccg gcaacggtgg tcaaggaaag atttcattta
     7381 gacgcgaaaa atacgtaccc aagggtgggc cagacggtgg tgatggagga gatgggggat
     7441 caatcattat tagagccacc aaggccttat ccacactgcg tccctatgct ggtgttaaag
     7501 aaattgtcgc tgaatctggt cagccaggtg ctaagcgtaa acagcatggc caaaaaggtg
     7561 aagacagaat tattgaagtg cctgttggta ccacagttac tttgttggca gaaaaccaca
     7621 ccagtgccta tcggcgtcaa cgttatggtt ttgtcaatca agacggaagt cagcgcttgc
     7681 tacgttctgc cgattttcaa gctggtcaag tgcctccagg aactaatatt aaacgccaac
     7741 gttacttcat tgattatgag gtgagtgata gcaatttagt tttagaacct gatgaattag
     7801 aagccctgcc acaaccagaa gaactttgtc agttaatgga agatggtgaa gaattagtac
     7861 tatgtcaggg aggttttggt gggcgaggta atagtcaatt taaaagtagc caaaaaacta
     7921 ctcctagaga ggcagaacat ggtacttttg gtgaacaaaa attggtaaaa ctggaattaa
     7981 gactattggc tgatctggct ttagttggtt ttcccaatgc tggtaaaagc acttttttat
     8041 ctaaagtaac ggcggccaat cccaaaattg ccaattaccc ctttaccacg cttgagccga
     8101 atttgggaat catgcaattg caaaaaggtg ctcaagaaat tgtggtggcc gatattcctg
     8161 gtttaattga gggggctaat cagggcaagg gtttgggtca tgactttttg cgccatattg
     8221 agaattgtca agcattaatg tacgtactgt ttttagaaga aagtgttatt tttgatcaag
     8281 cgctgtcagc caaggataaa agtcaattgc tttgggaaca ataccaaaat cttaacaatg
     8341 aactcaagca acacaatcct cagttgttag aaaaacctta tttagttact ttgaataaag
     8401 ctgatttgta tagtcaagca gaaattgaac agtttcaggc tgatttcgcc aaacacaaaa
     8461 tcactttatt agtgtttagt gccatcactg gtgagggttt gtccgcagtt gtaaaaaata
     8521 ttgagaagct ttttttaata actaagacaa gtttatcgag taagaattat tgattatcaa
     8581 tagtctcttc ttcaggcact aaactacttt cgcgcaacaa attagaagtt tcttcttcgt
     8641 tgagtaaatc agacaaacca gattcagttg cagtagctgg ttctaagcca gtttgctgat
     8701 tggttatgtc tggttctacc tgcttagcgg gagctgctgg agtggttaat tcctcctgtg
     8761 gggtttgttg accctgctgg gcttgttgag cttgttgagc ttgttgggct tcagcttgag
     8821 ctcgcaattg atccgcctct tcactaatcg actctagcaa ggttttcaca gtgttgtaat
     8881 cttcaagatt agtttcttga tcaattaaca ataaggcttg ctgtagcgcg ttttctgctt
     8941 ccaagatttg ttgatttttg atgtaggcct gggctagatt gtagtaggcc acaggtaaat
     9001 ctggtttgcg ttcaatagct tgaataaaga aattgctagc ctgttcccac tgctctaggc
     9061 gcatcatgaa ttgtcccaaa ttaattctca taaaggggtc attgggatta gtcagagcgg
     9121 cttgagaaag tgattcaaaa gccaattgac gagcatcttc agtaacatca agtagttgca
     9181 gataaatatt ggctaaagtt aaccagtttt gataagaacg gggctgcaaa atcgttgctg
     9241 ctttggcttc ggtaatggcc acattgatca attcaattac ttgctcttgt tcgactggag
     9301 taatatctgt tttatttgac aaagctacag ctaattccaa agcactcgac gcataaattc
     9361 ttctccaatc ggctgaacga ctagctaaat ttcttgattg ttcttgttta gcaaaaactt
     9421 cattaaattt ttcttcacta gcggccttac tcgcttgata ggcggcagca tgagcggcat
     9481 aatgcagacc aatttggtaa gtcaagaaaa ttaaaccagc catcaataac acagaaatgg
     9541 tggcaaaagt atagcgatta gccttgttct ctggttgtag gttaaaagga tcaaaaagat
     9601 tgttaaaaga aaactttttg attttataac tatctaaatt agctgctaaa taaaagctca
     9661 gagctatggc taataaggtc aacatcatgg tatt
//
LOCUS       DAOSEW010000018         9691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_5981_length_9691_cov_4.264944, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000018 DAOSEW010000000
VERSION     DAOSEW010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 9691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9691
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5981_length_9691_cov_4.264944"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="PLJ02_01145"
     CDS             complement(<1..1126)
                     /locus_tag="PLJ02_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOI05059.1"
                     /translation="MEKSYLDYAMSVIVSRALPDVRDGLKPVHRRILYSMYKSGIHHN
                     GPYKKSARIVGDVLGKYHPHGDSAVYMALVRLAQDFNMRYPLVDGQGNFGSIDGDSPA
                     AMRYTEARSAKITQELLADINKDTVDFVDNFDGSLKEPLVLPTRLPNFLLMGSEGIAV
                     GMATKIPPHNLTESCNAVAGMVKKGKAISDIQINVDEKKLNSNSECINTLLDSDPRQL
                     AGHFESEITHDELMTFIKGPDFPTAGIIYNQKAISEAYRTGKGRIIVRGKASIEENKR
                     GQMEIVITELPYQVNKARLLMKIADLIRDKKISGIKNLRDDSDRAGMQITVELKKDAR
                     PKVILNKLFKMTELQSSFSLNMVALNSDGTPQLMTLSQVLK"
     gene            complement(1290..2237)
                     /locus_tag="PLJ02_01150"
     CDS             complement(1290..2237)
                     /locus_tag="PLJ02_01150"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF003877.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HOI05060.1"
                     /translation="MKTYVIGHLKPDTDSVVAALALAEFYRHTPSFNRPNAEAVIAEP
                     INNETAFLLNKFAVASPKIITAKDIQPEDQIVLVDHNEAYQRLVGINDEQIVEIIDHH
                     KPNLTLNQPLFMTFKSWGSSSTVVYYLLKENDFTPDKKLASLLLSAILSDTVGFKSST
                     CTDQDIKTAQKLAKLAGIDDLEALTLEIFKAKSDLNQLSCQQMVKNDYKIFDFAGKKV
                     MIDQLETVEQEQLITEKKECLLEAMQEVKEELGVALIFVVISDILQVNSKILVLGDHE
                     QAIAESAFATKVTDHVIDIGAKLSRKKDIAPAIERAITK"
     gene            complement(2247..4302)
                     /locus_tag="PLJ02_01155"
                     /pseudo
     CDS             complement(2247..4302)
                     /locus_tag="PLJ02_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
     gene            complement(4450..5121)
                     /locus_tag="PLJ02_01160"
     CDS             complement(4450..5121)
                     /locus_tag="PLJ02_01160"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05061.1"
                     /translation="MPNFYISEILFLTKFAEQKPTNNLKFAEIKDLVVDYLQKKYPRE
                     LVSNNNVFWYNSDLQSFKIALAREIIQEAQYSNWDQERIRILVLLNFETASVAAQNAL
                     LKLIEEPPANTLIVLPVINSHRLLATISSRCSLCQINQENVETDKKTKTNDFTWPSNL
                     AEVFNTIDRYKDRDSAKNFVRNLLEQKQLGYKEKKALAQAYFDLESNLNVRLALEHCF
                     FSFFQ"
     gene            5152..7476
                     /locus_tag="PLJ02_01165"
     CDS             5152..7476
                     /locus_tag="PLJ02_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOI05062.1"
                     /translation="MLTAQNNQSDEVGQGQSVAEPDKSQQNQVENGDVQVDPDLQRTI
                     NQITQDLNIQPVYGDHVSQLPPVDLAADAKQGQVPSQPQPLAQSQPLAPTQPLAPTQS
                     IEPAQPAEPEVRTEPEVEAESYGGNNTLGNPDDLGIAEPVFTQPIPTDDLQPVPTDGL
                     QSVLTNGLQPIPTDDLQPIPTIANPPNSVAPAGPAFAMTNSFFDILVQKGIISPQQAE
                     EIRQENLNTGRSYEELVSSKQLATETEIVQAKAEFNNVPYIKLATIGVDPEALTLIDE
                     GIAKRFQALPFSVDKDKKILKIAMINPLDLAAIEFLKQKTGYNISAYYSEATEIARMI
                     DDHYAQSLSSEVTEALKSTTQDREGESGSVELDQQNGGILRDAPINRIVENILDFAIK
                     SRASDVHIEPQINRTRVRYRIDGILAEKLILPLNVHEAVVSRIKIMANLKIDEKRLPQ
                     DGRFNYKTNKAEIDLRISTLPTVHGEKVVMRLLQKDMAVPSLEDLGLTGLGLQRVMEA
                     IKIPYGIILITGPTGSGKTTTLYSILNIINKVGVNIMTLEDPVEYQIPGVSQVQTNPK
                     AGLTFASGLRSFLRQDPDIIMVGEIRDNETADLALQASLTGHLVFSTLHTNSAAGALP
                     RLLDMEAEPFLLASSMTLSMGQRVVRRINDQYKEEYKPEPIVIEDIKKVLGVHFDRWC
                     KEKGKKPEEITLFRPSNKRPVTEPEYKGRIGIFEVMPVSPEISSLILNRKPASEIERV
                     AIQQGMTLMKQDGYLKVLDGITTVEEILRVAEVG"
     gene            7538..8599
                     /locus_tag="PLJ02_01170"
     CDS             7538..8599
                     /locus_tag="PLJ02_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOI05063.1"
                     /translation="MNIKEILQFLIDNEGSDIHIIPGSPLSIRINGILSFPAGPQLLG
                     PDQTEPWIFDLLSEEQKKKLKEEKEIDLAYQFGNNARFRVCVYQHLGQYAAALRLIPQ
                     TIKNIDELALPKIFHTFGEYNQGLVLFTGPTGEGKSTSLAAIIDEINHNHSRHIITIE
                     DPVEFVYKPDKSVVSQREVGKDTHQWHLALRSALRSDPDVVLVGEMRDLETIAATLTL
                     AETGHLVFATIHTNTAAQTIDRIIDVFPAHQQGQIRQQLASVLKAIVSQRLVTKIQGG
                     RIAAVELLFNNPAVANLIRDEKVFQIDTVIQTNVNSGMMLMETHLLELYKQHVISKET
                     AISSAFRPTDMMRLLDEEG"
     gene            8608..>9691
                     /locus_tag="PLJ02_01175"
     CDS             8608..>9691
                     /locus_tag="PLJ02_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOI05064.1"
                     /translation="MPIFKYTAKNKRGENIKGKVEAASKLQAVSTLFERGLFVIDVSS
                     LGQESGFLAKFGNAKVKFNDLVNFTRQLATMINAGLPLANALSILEEESEKEMGKLTA
                     NLLKDVEGGMNFSDALQKYPDNFSRIYTQLVKAGEAGGVLDEVLDRLAITLEKDKDFK
                     AKTKSAMIYPVIILVVMVIVMIVMMVAVIPQLTEMYNDFGAELPMMTQIFISISDFMV
                     QFWWMILVVVGAGIFAVRSYRKTKKGELAIDGWLLKLPIFGKMRQKIILTDFTRTLSL
                     LLGAGVSLLEAINIVAEAIDNAVYRQELAEAAKQVERGTSLSTAISRYEDFPPILHQM
                     MSVGEETGKLDEILGKLAEYFEKESEY"
BASE COUNT         2831 a         1927 c         1967 g         2966 t
ORIGIN      
        1 cttttaacac ttgagataaa gtcatcagct gaggggtgcc atcactatta agtgcgacca
       61 tattcagcga aaaactgctt tgcaattcag tcattttgaa aagtttattc aagattactt
      121 ttggtcgcgc atcttttttc aattccacgg tgatctgcat cccagcacga tctgaatcat
      181 cacgcaaatt tttaatacca ctaattttct tatcccggat caaatcagca attttcatca
      241 gtaaccgagc cttgttgact tgataaggca actcagtgat cactatttcc atctgccccc
      301 gtttgttttc ttcaatactt gctttaccgc ggacaataat tcgcccctta ccagtgcgat
      361 aggcttcgct aatggctttt tgattataaa taatgccagc agttggaaaa tctggtccct
      421 taataaaagt cattagttca tcgtgggtaa tctcactttc aaagtgtccc gctaactgac
      481 gagggtcgct gtccagtaaa gtattgatgc actccgagtt tgagttcagt tttttttcat
      541 ccacattaat ctgaatatcg ctgatcgctt tgcctttttt gaccatacct gctactgcat
      601 tacagctttc agttaaatta tgtgggggaa ttttggtagc catgccaacg gcgattcctt
      661 ctgagcccat cagcaagaaa tttggcaggc gggttggtag caccagcggt tctttgagcg
      721 agccgtcaaa gttatcaaca aaatcaaccg tatctttatt gatatcagcc aacaattctt
      781 gagtaatttt ggcacttctg gcttcagtgt aacgcatggc tgctgggcta tccccatcaa
      841 ttgagccaaa gttaccttga ccatcaacta aaggataacg catgttaaag tcttgagcta
      901 aacggaccag agccatataa acagcactgt caccgtgggg atgatattta cctaacacgt
      961 caccgacaat tctggccgat tttttgtagg gaccattgtg atggatacca gatttataca
     1021 tggaatacaa aattcggcga tgcaccggtt ttaaaccatc tctcacatcg ggtaaagcgc
     1081 gggaaacaat gacgctcata gcataatcca aataggattt ttccatttca tcaacaatat
     1141 cggtgtgttt gagggtgccg tatttactct cttcagtagt gccaaatttg gcactgggat
     1201 catcgttact gatattggct ttggtttctt cggtgttgct tggcttggtt tcttctgaca
     1261 tgttacttaa tcttttgata tcttaattat tattttgtta tagctcgttc aatggctgga
     1321 gcaatatctt tctttctgga taatttagcg ccaatgtcaa tgacgtgatc ggtgactttg
     1381 gtcgcaaaag ctgattcggc aatggcttgt tcgtggtcac ctagcacgag aattttacta
     1441 ttgacctgga gaatatcgga aattaccaca aaaatcaggg ctactccaag ctcttctttt
     1501 acctcttgca tcgcttcgag caaacactct tttttttcag taattaattg ttcttgttca
     1561 actgtctcaa gctgatcaat cattactttt ttcccggcaa agtcaaaaat tttataatca
     1621 tttttgacca tttgctgaca acttaactga ttcagatcag atttagcttt aaaaatttct
     1681 aaagttaagg cttctaaatc atcaatgccc gccaacttgg ctaatttttg ggcagtttta
     1741 atatcttgat cagtacaagt actcgattta aaaccaacgg tgtcagacaa aatagccgac
     1801 agcaacagac tggcgagttt tttatctgga gtaaagtcat tttctttcaa caaataatag
     1861 accacggttg aggatgatcc ccagctctta aaagtcataa aaagcggttg attcaatgtt
     1921 aagttgggtt tatggtggtc aataatttcc acaatttgtt cgtcgttaat ccctacaagt
     1981 cgttgatagg cttcattgtg atcaaccaaa acaatttgat cttctggctg aatatctttg
     2041 gcggtaatga ttttgggact agcgacagca aacttgttga gtaaaaaagc tgtctcatta
     2101 ttaattggtt cagcaatcac cgcctcagca ttgggccggt tgaaagaggg agtgtgtcga
     2161 tagaactcag ccaacgccag ggctgctacc actgaatctg tatctggttt aagatgaccg
     2221 atgacgtaag ttttcataaa tttcctttat acatcaagct ctgccatttt ggctcggctt
     2281 tgaataaatt ttttacgagg ggccacttcg ctccccatta acacatcaaa tgtagtgtca
     2341 gctttattag catcttcaat atcaattttt ttcagcacgc gacttgctgg attcatggta
     2401 gtgctccaga gttgctcagg gttcatttcc cccaaaccct tgtagcgctg gatgccaatt
     2461 ttggcattag ggtttgttgc caaaattttc ttaatctgca tctcacgctc ttcttcactg
     2521 taagcataaa actcctcttt gttaaaagta attttaaaaa gaggtggcat tgccacgtac
     2581 agataaccct tttcgatgac ctctggtaaa tggcgaaaga aaaaagtcag tcccagagta
     2641 gtaatatgtt caccatcaac atcagcatca ttcattaaaa taatgcgatg ataacgtaat
     2701 tttgtctcat caaaactatc gccaatccca caaccaagcg caatcactaa atttttgagt
     2761 tcatcactag ccacgatccg atcaaggcga gcccgctcag tgtttaaaat tttaccgcgc
     2821 aaagggaaaa tagcctgaaa atggcggtca cgaccctgtt tggcactccc acccgcacta
     2881 tcgccctcaa cgatataaat ttcgctttct tctggtttat tagattggca gtcggctagt
     2941 ttgcctggca gcgacaggcc ttctagtaat cctttgcgga tgacggcgtc tttggcggcg
     3001 cgagccgcta ggcgagcgcg agcagaaatt aacacctttt ctacaatggt tctagcaact
     3061 tttggatgtt cttctaaata agtagcaaaa tgttttttaa agacacgata aaccgcagtt
     3121 tgcgcttcag cgttatttaa tttagccttg gtttgagatt caaattgtag atcattggaa
     3181 ggcattttga caaaaaccac cgccgtcagg ccttctctta aatcatcagc gacgaagttc
     3241 tctttttcct taatcatgcc attcttttct gcatagtcac gaatgacccg tcccaaagct
     3301 aagcaatccc tgaacatgag taccaccatc tggagtgtta atcacattga cataactctc
     3361 cacccgttct ttgtaagagt cattgtattg cagggcaatc tcaatcccaa tttctttttt
     3421 cgtttcttca tcggtccatt ggtcattgag ataaaaaact cggtgtagag attttttgga
     3481 catattaatg tgctcgacca aggaccggat tcccccctca aaataaaaat gctgttcaat
     3541 gttggtaacg cgatcgaaaa gttggaagta aataccggcc atcagataag cacgatcttt
     3601 aacaatatca atgatagttt ttgggttata gctaatttgt ttaaatatca gtggatcggc
     3661 aatgaattgc actatggtgc cactttcata gttgacaaaa tcctggcttt gttgaggaaa
     3721 tttggcaatt aattctgttt cgctgatttc tgttactggc gccgccggaa ttcctcgcga
     3781 atgatattga tagtaatatt tattattacg tttaacagcc acttgcatta gacaagacaa
     3841 ggcgttgaca gcactagaac caataccgtg caaaccaccg gatactttgt aagctttact
     3901 ttcaaatttg cctccagcat ggagtttagt catcacgact tccatggcag acacacctga
     3961 agcatgcttt tcgaccggaa tgccccggcc gttatcaacg acggtaatga cttgatcagc
     4021 taattctttt tcttcagcct tgagtttttt attagcttgg tgtttcaaga ataattcccg
     4081 atgcaaatca ctaattgggt cgcggaataa ttggatagta tcaccaaagc cagcaatcgc
     4141 ttcatcaact gcgttgtcca aaatttcttt aactagatga tgcagaccat tagcatcggt
     4201 agaaccgata tacatgcctg gccgttttcg caccggttcc aggccttcta agatcttaat
     4261 ttgctctgct tgatagttat taatatcact ctgacttgcc ataagaacta catttgtagc
     4321 atatttatta tataaaagaa agaccaaaaa agctcacgga gcttgaaaaa aagccgattt
     4381 tgggcgtttg agacgctcga ttttaaaaaa ctccaggcta aactatctgg tagtactcat
     4441 tactatcaat tattggaaaa aactaaaaaa acagtgttcc agcgctaaac gaacattgag
     4501 attgctttct aaatcgaaat aggcttgagc tagggctttt ttttctttat atcctagctg
     4561 tttttgctca agtaagtttc tgacaaaatt tttggcgctg tcccgatctt tatatctatc
     4621 gatagtatta aaaacctcag ctagattact tggccaagta aaatcattag ttttggtttt
     4681 tttgtcagtt tctacatttt cttgattaat ttggcagagg gaacagcgtg aactaatggt
     4741 ggctaataaa cgatgagaat taatcactgg taacacaatc aaggtgttgg ctggtggttc
     4801 ctcaatcaat ttgagcaaag cattttgcgc tgctacactg gcagtttcaa aattgagtaa
     4861 aactaaaatg cgaattcgct cttggtccca attactgtat tgtgcttctt gaataatttc
     4921 tctggctaag gcaattttaa agctttgcag gtcgctgtta taccaaaaaa cattattgtt
     4981 ggagactagt tctcgaggat attttttctg cagataatcc acaaccaaat ctttgatttc
     5041 agcgaattta agattatttg ttggcttttg ttcagcaaat ttagtcaaaa acaaaatttc
     5101 gcttatgtaa aagtttggca ttttatggca ttataaaagg tataagcgat tatgttaact
     5161 gcccaaaata atcagtcaga tgaagtgggt cagggtcagt ctgtggctga acctgacaag
     5221 tcacaacaga accaagtcga aaatggtgat gttcaagttg atcctgattt acaaagaacc
     5281 atcaatcaaa ttactcaaga tttaaatatt caaccggttt atggtgacca tgtttcccag
     5341 ttgccaccag ttgatttggc tgctgatgca aaacagggcc aagtgccaag tcaaccacag
     5401 cctcttgcgc aaagtcagcc acttgcgcca acccaaccac ttgcgccaac ccaatcaatt
     5461 gaacctgctc agccagccga accagaagtc agaactgaac cagaggttga agccgagtct
     5521 tatggtggca ataatacttt gggcaatcca gatgatttgg gcattgctga accggttttt
     5581 actcagccca tcccgactga cgaccttcaa cccgtcccaa ccgatggttt acaatccgtt
     5641 ctcactaacg gtttacagcc catcccgact gacgatcttc aacccatccc gactatcgcc
     5701 aatccaccaa attcagtagc tccagctggt cccgcttttg cgatgaccaa ttcctttttt
     5761 gatattttag tccaaaaagg gatcatttca ccgcaacaag cagaagaaat tcgccaagaa
     5821 aatctaaata cgggtcgcag ttatgaagaa ttagtttcaa gcaaacaact ggcgactgag
     5881 acggaaattg ttcaagccaa agctgaattt aataatgttc cttatataaa attagcaacg
     5941 attggcgtcg atccagaggc tcttactttg attgacgagg gcattgccaa gcgttttcaa
     6001 gctctccctt ttagtgttga taaggataaa aaaattctca aaattgccat gattaatcct
     6061 ttagacttgg cggcgattga atttttgaaa caaaaaactg gttataacat tagtgcttac
     6121 tacagcgagg ccactgaaat tgctcgcatg attgatgatc actatgcgca aagtttgtct
     6181 agtgaagtga cagaggctct gaaaagtacc acacaagacc gagagggaga gtctgggtca
     6241 gtggaattgg atcaacaaaa tgggggaatt ttgcgggatg caccaattaa ccgtattgtg
     6301 gaaaatattc tagattttgc tattaaatca agagcctcag atgttcatat tgagccacaa
     6361 atcaaccgta ctcgggtgcg atatcggatt gatggaattt tggctgaaaa gttgatttta
     6421 cctctgaatg tacacgaggc agtcgtctcg cgtattaaga tcatggctaa tctcaaaatt
     6481 gatgaaaaaa ggctacctca agatggacgt tttaactaca aaactaataa agcggaaata
     6541 gatctccgta tctctactct gccgacagta catggggaaa aggtggtgat gcggttgtta
     6601 caaaaagata tggctgttcc ttctttggaa gatttaggtt tgactggttt gggtttgcag
     6661 agagtgatgg aggctatcaa aataccttat ggcattatct tgattactgg tccaactggt
     6721 tcaggtaaaa ctacaaccct ctattcgatt ctgaatatta ttaataaggt gggagtcaat
     6781 attatgactc tcgaggatcc agtggagtac caaattcctg gagtgagcca ggtgcaaacc
     6841 aatcctaagg ccggtctaac ttttgcttcg ggattgagat cttttttgcg tcaggatccg
     6901 gatattatta tggtgggtga aattcgtgat aatgaaactg ctgatttggc actacaagca
     6961 tctttaactg gtcacttagt cttctctact ctacatacca acagtgcggc tggtgcctta
     7021 cctcgtttat tagatatgga agctgaacca tttttacttg cttcatcaat gactttatcg
     7081 atgggtcaac gtgttgttag aagaattaat gaccagtata aggaggaata taaacctgaa
     7141 ccaatagtga tcgaagacat taaaaaggtg ctaggagtcc attttgatcg ttggtgtaaa
     7201 gaaaagggta aaaaaccaga ggaaattact ctttttcggc ccagtaataa gcgaccagtg
     7261 accgaacctg aatacaaagg tcgtattggt atttttgagg ttatgcccgt ttctccagaa
     7321 atttctagct taattttaaa tcgcaaacca gctagtgaaa tagaaagagt cgctattcaa
     7381 caaggcatga ccttgatgaa gcaagatgga tacttaaaag ttctagatgg tatcaccact
     7441 gtagaagaga tattacgggt ggcagaagtt ggttaagcaa ttaaagattg aattaaaagt
     7501 gatcaagtat atataatgaa taataagaaa aatagctatg aacattaaag aaatattaca
     7561 gttcttaata gataacgaag gttcagatat tcacattatt cctggatctc ccctgtccat
     7621 caggatcaat ggaattttga gttttccagc tgggccccag ctactgggtc ctgaccaaac
     7681 cgagccgtgg atctttgatc tattaagcga agagcaaaaa aagaaactaa aagaagaaaa
     7741 agaaattgat ctagcttatc aatttggcaa caatgctcgt tttcgtgttt gtgtttacca
     7801 acatttgggt cagtatgcag ccgctctgcg cttaattccc caaaccatta aaaatattga
     7861 tgaattagct ttgccgaaga tttttcatac atttggtgaa tacaatcaag gcttagtgct
     7921 gtttactggt ccgactggag agggcaaatc cacttcattg gcggcgatta ttgatgaaat
     7981 taatcataat cacagtcgac atattattac tattgaagat ccagtagaat ttgtctataa
     8041 gccagataaa agtgtggtct ctcagcgcga agtcggcaaa gatacccatc agtggcattt
     8101 agccttgaga agcgccttgc gttccgatcc tgatgtggtt ttggttggtg aaatgcgtga
     8161 tcttgaaaca attgccgcta ctctcactct agctgaaact ggtcacttag tttttgccac
     8221 cattcacacc aatacggcgg cacaaaccat tgaccgaatt attgacgttt ttcctgctca
     8281 tcagcaaggt caaattcgtc aacaactcgc cagtgtctta aaagcgatag tgtcgcagcg
     8341 cctggttacc aaaatccaag gtggccggat cgcagcggta gagctgctgt ttaataatcc
     8401 agccgtagct aacttgattc gtgacgaaaa agtttttcaa attgacactg ttatccagac
     8461 caatgtcaac agtggcatga tgttaatgga aactcatctt ttggaacttt ataagcaaca
     8521 tgttattagt aaggaaacag ccattagtag cgcttttcgc cccaccgata tgatgcgatt
     8581 attagacgaa gaaggataag gtttattgtg ccaattttta aatacacagc caaaaataaa
     8641 cgtggagaaa acatcaaagg taaagtagaa gctgcctcaa aattacaagc tgtttcaacc
     8701 ctttttgaac gtggtttatt tgtcattgat gtttccagtt tgggtcaaga atcgggattt
     8761 ttggcaaaat ttggcaatgc caaagtgaaa tttaatgatt tggtcaattt cactcggcag
     8821 ttagccacga tgatcaatgc tggtttgcct ttggcaaatg ctttgtcaat tttggaagag
     8881 gaaagtgaaa aagaaatggg taaattaacg gctaatttat taaaagacgt ggaaggtggc
     8941 atgaattttt cggatgcact gcaaaagtat cctgataatt tttcccgcat ctatacgcag
     9001 ttggtaaaag ctggcgaagc tggaggtgtt ttagatgaag tattggaccg gttggcgatt
     9061 actttagaaa aagacaaaga ttttaaagca aaaaccaaaa gcgcgatgat ttatccagtc
     9121 attattttag ttgtgatggt gatcgtgatg atcgtcatga tggtagcagt gattccgcag
     9181 ttaaccgaga tgtacaatga ttttggagca gaattaccca tgatgactca aatttttatt
     9241 agtatttctg actttatggt gcaattttgg tggatgatct tagttgtagt aggtgctggg
     9301 atatttgcgg tgcgttcgta tagaaaaacc aaaaaaggag agttagcgat tgatggttgg
     9361 ctattaaaat taccaatttt tggcaaaatg cgtcaaaaga ttattctaac cgattttact
     9421 cgtactcttt ccttgttatt aggagctggt gtgtcactgc tagaggctat taatattgtg
     9481 gctgaagcga ttgataatgc tgtttaccgt caagaattag ctgaagcagc taagcaagta
     9541 gaacgtggta cttccctttc caccgctatc tctcgttatg aagattttcc gccaattctt
     9601 catcagatga tgagtgttgg tgaagaaacc ggtaaacttg atgaaatttt gggtaaattg
     9661 gctgaatatt ttgaaaaaga aagcgaatat g
//
LOCUS       DAOSEW010000019         9600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_6052_length_9600_cov_4.507177, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000019 DAOSEW010000000
VERSION     DAOSEW010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 9600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9600
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6052_length_9600_cov_4.507177"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..97
                     /locus_tag="PLJ02_01180"
     tRNA            23..97
                     /locus_tag="PLJ02_01180"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56..58,aa:Glu,seq:ttc)
     gene            complement(166..1035)
                     /locus_tag="PLJ02_01185"
     CDS             complement(166..1035)
                     /locus_tag="PLJ02_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOI05065.1"
                     /translation="MNNIKLSQAKAMKQLYAFTTKRAIDLSLSENPLGCSPLVLAASR
                     NLAVNINDYPLTNGCLLKKALADHFTLSENNFFIANGSEAIINYLAKVFGRVGDEVLV
                     PALTFPLFAISTKLAGKKLVKVKMTTNLGINLVAMRKAISAKTCLIFLCNPNNPTGAV
                     LPQQAILDFLDCVPKSVLLVVDEANIEFAGESVISEIKNRDNLLVLRTFSKGFGLAGL
                     RVGFATANNLFVKISDRLSEQQFKKSLQTNDTSVVMGSSFAGFSDAFFRISVRSHNIN
                     QRFIQKISRPAVN"
     gene            complement(1198..3216)
                     /locus_tag="PLJ02_01190"
     CDS             complement(1198..3216)
                     /locus_tag="PLJ02_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI05066.1"
                     /translation="MSQSQHTTKYQFRPVRGPGGGRGNMLARGVGEKAKDFRGTVKQL
                     INYLRPYWFKLALVLVFTILSTLFSIVGPKILGRATNQIVSDYVDIKDYELLTSILSV
                     DGQLPTGTTGAELIKQLSPQQLDSIPAKTLENIQTLDLSQRPSFHFADLRNIIMLLIG
                     LYVLSATFSYIEGWVMTGVTQKVTYQLRKDISQKINRLPLKYFDKHTYGEVLSRVTND
                     VDTVNHTLNQSISQIISSLVMIVGVVVMMLSISWLLTVVAILILPLSLILIRQITKRS
                     QPQFAKQQLVLGYLNGHIEEIYSGHVIVKAFNGSRDAINKFKQINADLYDSAWKSQFL
                     SSLLRPLMVFVGNLGYVGVAIVGGWLAISGKLQIGDIQAFIQYVQQFNQPIAQAANIS
                     NVMQSMVAAAERVFEFLAVKEQIPDVDNPVVLSTVKGQVEFDHVVFAYNPGQTVIKNL
                     SVKIKPGHRIAIVGPIGAGKTTLVNLLMRFYEIDSGAIRIDGVDIKQMRRSDVRQIFG
                     MVLQDTWLFNGSIRDNITYGNPQASEEELLKVSREAHIDHFIQSLPNGYDMVINEDAS
                     NISQGEKQLLTIARAMLAGNPMLILDEATSSVDTRTEVLIQKAMDTLMKGKTTFVIAH
                     RLSTIRDADLILVMKDGDVIEQGTHQELLKLKGFYASLYNSQFTDDYT"
     gene            complement(3213..4952)
                     /locus_tag="PLJ02_01195"
     CDS             complement(3213..4952)
                     /locus_tag="PLJ02_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI05067.1"
                     /translation="MIKLIKFLKPYIWLILALIVVTYFQVMANLKLPEYMAKIVNEGI
                     LLKNVDIIYHHGWLMLVVTLLGAICAVLVGFLASRIATGFARDIRQEVFTKVESFSVS
                     EFNKFSTASLITRSTNDVQQMQMTLTMIFRIVLMAPIMAVGALQKALATAPSISWIIA
                     LATLLLLTMILALFIFGLPKFKLVQELIDKLNLVTRENLSGLKVIRAFNNESREEAKF
                     EKTNQELTRANLFVNRLMSILQPTMNLVLNFAVIAIVWFGAHLIDSGSLQIGEMLAFI
                     QYTMQVIMSFLMISIVFIMVPRAAVSGKRIVELLETEPLIKDPETPIKPNAQGKGRIE
                     FRDVTFTYPDADLPVLTAINFTAEPGQTTALIGSTGSGKTTLINLVPRFYDVTAGQIL
                     IDGVDIRDLKLEDLYRQIGYVPQKGVLFSGTVKSNISFGNKQLSDAEIKEAAEIAQAT
                     EFINKLNKKYDSEIAQGGANVSGGQKQKLSIARAIAVKPKIYIFDDSFSALDFKSEAK
                     LRRQLLQKTKGQTTLIVSQRISTIMNVDKIIVLDQGKIVGQGTHRELLKNNSVYQEIA
                     YSQLSDEELKLQS"
     gene            complement(5131..5496)
                     /locus_tag="PLJ02_01200"
     CDS             complement(5131..5496)
                     /locus_tag="PLJ02_01200"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOI05068.1"
                     /translation="MRPEDPDSPEQSKKRQAWFTAFETRLITKLPKHLQQEIKFQVVG
                     SVALGRAGADSDIDVVIRYRGSEELVLPQVRQAIVSLLEEMKQAGEPTFKIDIHDVDY
                     PLMFSELALLGKSKRDPAK"
     gene            complement(5555..5944)
                     /locus_tag="PLJ02_01205"
     CDS             complement(5555..5944)
                     /locus_tag="PLJ02_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOI05069.1"
                     /translation="MSGYSINLEEKTLNNQNFREVLYTAAHSQLVVMSIAPGDDIGME
                     THEEHDQFIRVEKGVGQAIIGESTYDLSDGVAIVVPAGQRHNVINASNTETLQLYTVY
                     SPAEHPEGKIHPTKQDALNDPDYAAEH"
     gene            complement(6021..7523)
                     /gene="gpmI"
                     /locus_tag="PLJ02_01210"
     CDS             complement(6021..7523)
                     /gene="gpmI"
                     /locus_tag="PLJ02_01210"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOI05070.1"
                     /translation="MSKDSQKKLALIILDGWGYYKDYPGNAISLANTPTWDKIWADSP
                     RALLACSGEAVGLPAGQMGNSEINHAAIGSGRVIYQDLVKLNKAVDEKTLHQAEAIKE
                     AFTRAKNNSSSLHIMGLLSPGGVHSHEKHIFGLIKEAADFGVKKIYVHAMTDGRDTAP
                     RSCLASFEKLAKICQETGAELASISGRYYAMDRDHNWERTDLAYSAIKDRQAPQFASA
                     QQAIADSYKNNKGDEFIVPCTIKVRDEEEAKVKSGDSVIFANFRNDRPRQLTERFLEK
                     GPENIFFVTMTLYSDQYPVAVAFGPEKIERTLGEILAENHIKQFRVTETEKFAHLTFF
                     LNCKREEPYPLEERFMFDSNKVESHAEKPEMKALAIADKIVETMNRGEHSVIFANICN
                     GDMVGHTGDIQAAIKAAETVDLALAKIKEAAETNNYFLIITADHGNCEEMLNEKGEVV
                     TEHSMNPVPFVLLNDGQLNRNEGMMKDIAPTVLKLLDLPQPKEMTGQSLV"
     gene            complement(7584..8882)
                     /gene="eno"
                     /locus_tag="PLJ02_01215"
     CDS             complement(7584..8882)
                     /gene="eno"
                     /locus_tag="PLJ02_01215"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOI05071.1"
                     /translation="MIIKSIKAQEILDSRGNPTVAVNLKLEDGTEVFSAVPSGASTGA
                     YEALELRDGDAGRYLGKGVLKAVANVNEVIAPKIIGQQVTEQEKIDQIMLDLDGTQGK
                     QNLGANAILAVSMACVKAGAIASKMPLYAYIAQLFGNSTDKFIMPIPMSNVLNGGKHA
                     IGGADMQEFMIFPLAAQSIAEAVRWNAEIFQHLGKIIEKKGWQKTVGDEGGYAPSLGT
                     NEAPLQLIVQAIEMAGYKPGEEVFIALDPAATEFYKDEGENSYYDLKTENKQLSSSDM
                     IKKYAKWVEEYPIVSIEDGLAEDDWAGFAQLTKTLGKKIQIMGDDLLVTNPERIQKAI
                     DQKAANSALIKLNQIGSVSETIQAIKLARSAGWTCVVSHRSGETEDTFIADFVVGAGT
                     GQIKTGSMCRSERIAKYNRLMMIERELGDKATMAKFPFHK"
     gene            complement(8911..8984)
                     /locus_tag="PLJ02_01220"
     tRNA            complement(8911..8984)
                     /locus_tag="PLJ02_01220"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8948..8950),aa:Cys,seq:gca)
     gene            9091..>9600
                     /locus_tag="PLJ02_01225"
     CDS             9091..>9600
                     /locus_tag="PLJ02_01225"
                     /inference="COORDINATES: protein motif:HMM:NF013112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05072.1"
                     /translation="MSKKYFIKTYGCQANKSDAERMAALFEEEGLIASDSWQDCDKLA
                     VVTCSVRERAEQRVRAFLLKVAKFYQDKKLTKPEIIFSGCMIHHGQEHLKKILPMIDQ
                     IVPTNQMAFLKKAKRQDKLHAFVPISVGCNSYCTYCIVPYARGREKSRPFSDIMEEVN
                     GLVKEGYQEI"
BASE COUNT         2838 a         2023 c         1734 g         3005 t
ORIGIN      
        1 ttgtataatc cagaggtaac ttggccctgt cgtctagcgg ttaggacatc gggttttcat
       61 cccgacaact ggggttcgat tccccatggg gtcaccagta tctagttgtt caacgcgact
      121 tgttagcctg tgtaaaagag ctcgacggga agagttttat ttttattagt taactgctgg
      181 cctgctaatt ttttgtatga atctttgatt gatgttatga ctcctaacgc taattctaaa
      241 aaacgcatca ctgaaaccgg caaaactaga acccatcacc actgatgtat catttgtttg
      301 taagcttttt ttaaactgct gctcgctcaa tctatcagaa attttaacaa ataaattatt
      361 agctgtcgca aaacccactc ttagaccggc caagccaaag cccttagaaa aagttcttag
      421 aaccaataag ttgtcgcggt tcttgatctc actgatcact gattctccag caaattcaat
      481 gttggcctca tcaactacca gtaaaacact ttttggcacg caatcaagaa aatccaagat
      541 agcttgttgc ggcagtacag ctccggttgg attattagga ttgcaaagaa aaatcaaaca
      601 ggtcttggct gaaatagctt ttctcatcgc tactaaatta attcctagat tggtcgtcat
      661 ttttaccttc accaactttt tgccagccaa cttggtagaa atagcaaata aaggaaatgt
      721 taaagctggc actaacactt catcaccaac tctgccaaac acttttgcta agtaattaat
      781 aatcgcttca gaaccattgg caataaaaaa attattctca cttaaagtga agtggtctgc
      841 taaagccttt tttaataaac aaccattagt tagaggataa tcattaatat tgactgctaa
      901 atttcttgaa gctgccagca ctagaggaga acaccccaat ggattctcac ttaacgataa
      961 atcaatggct cgtttagtag taaaagcata gagctgcttc atagctttgg cttgactaag
     1021 ttttatatta ttcatttttg cctttccacg cacaaaaaaa gcctccctaa aggaggctgg
     1081 tgactagtgc ttaattttat aaaattacga cagcaataac acccacctcc cgaaaagggt
     1141 gtgataccaa gcgtagtggt gtgcgttatt gactaataac atggcgtaga ttatagctta
     1201 agtataatca tcagtaaact gactgttata tagagaggcg taaaaacctt ttaattttaa
     1261 taattcttga tgagtgcctt gttctattac atcaccgtct ttcataacta gaatgagatc
     1321 agcatctcta attgttgaca atcggtgagc aataacaaag gtggttttac ctttcattaa
     1381 cgtgtccata gctttttgga tcaaaacctc ggttctagta tctaccgagc tggtcgcttc
     1441 gtctaaaatc agcatcgggt tgccagccaa catagctctg gcaatagtca gtagttgttt
     1501 ttccccctga gaaatattgc ttgcatcctc attgatcacc atatcataac cattgggtaa
     1561 tgattggata aaatgatcga tatgggcctc acgggatacc tttaacaatt cttcttctga
     1621 ggcctgagga ttaccatagg tgatgttatc gcgaatggaa ccattgaaca accaagtatc
     1681 ctgaagaacc atgccaaaaa tttggcgcac atctgatcgt ctcatctgct tgatatcaac
     1741 tccatcaatt ctaatcgccc cactatctat ttcataaaat ctcatcagca aattaaccag
     1801 cgttgttttt ccagccccaa ttggtccgac aatagcaatt ctatgaccag gtttaatttt
     1861 aacagacaag tttttaatta ctgtttggcc tggattataa gcaaacacta cgtgatcaaa
     1921 ttctacttga cctttaactg ttgagagtac cactggatta tctacatctg gaatttgctc
     1981 ttttactgct aaaaattcaa acactctttc agctgctgca accattgact gcatcacatt
     2041 actaatatta gctgcttgag caatgggttg attaaactgt tggacatact gaataaaagc
     2101 ttggatgtct ccaatctgta actttccgct aatagccagc cagcccccaa caatggccac
     2161 ccccacatat cccagattac cgacgaaaac cattagggga cgcaataaac ttgataaaaa
     2221 ctgggacttc caagcactat catataaatc tgcattaatt tgcttaaatt tgttgatagc
     2281 atctcgactg ccattaaaag ctttgactat cacgtgccct gaatagattt cttcaatgtg
     2341 tccattgaga taacctaaaa ctaattgctg tttggcaaat tgtggctgag accgcttggt
     2401 aatttgccta atgagaatca agcttaaggg taaaatcaaa atagcaacta ctgttaaaag
     2461 ccaactaata gacaacatca tgacaacaac gcccactatc attactagcg aactgatgat
     2521 ttgagaaata ctttggttga gagtgtgatt gactgtgtca acatcattag tcacacggct
     2581 caatacctcg ccataggtgt gtttatcgaa atatttcagt ggtaaacgat taatcttttg
     2641 tgatatgtct tttcttaatt gataagttac cttttgagtt acaccagtca tcacccagcc
     2701 ctcaatataa ctaaaagtgg cactaagaac atataatccg ataaggagca taataatgtt
     2761 cctaaggtct gcaaaatgaa aactggggcg ctggctaaga tcaagcgttt gtatattttc
     2821 taatgttttg gcgggtatgc tatctaattg ttgaggtgat aattgtttaa ttaattcggc
     2881 ccctgtggta ccagtaggca attgaccgtc cacggataat atactagtta gtaactcata
     2941 atccttaata tcaacatagt cactcactat ttgattggta gcccgaccta atatcttggg
     3001 accaactatt gaaaagagag tactaagaat cgtaaaaact aatactagag ccagtttaaa
     3061 ccaatagggt cgaaggtaat taatcaattg cttgactgtc cctcgaaaat cttttgcctt
     3121 ttcaccaaca ccgcgtgcca gcatgttacc tctaccacca ccaggtcctc ttactggtcg
     3181 aaattgatac ttagtagtgt gttgactttg actcatgatt gtaatttaag ttcctcatca
     3241 gacagctgag aataagctat ttcttgatac acgctgttat ttttaagtag ttcccgatgt
     3301 gttccctgac ccactatttt gccctgatct aaaacaataa ttttatcaac attcataatg
     3361 gtgctgatcc gttggctgac tatgagggtg gtttgacctt tggttttctg taataactgt
     3421 cggcgcagtt tagcttctga cttaaagtcg agtgcagaaa aggaatcatc aaaaatataa
     3481 atttttggct taacggcaat ggctctggca attgataatt tttgcttttg tcctccagaa
     3541 acattggccc caccttgagc tatctcgctg tcatattttt tatttaactt atttatgaat
     3601 tcggtggctt gggcaatctc agctgcttct ttaatttcag catcactgag ttgtttattg
     3661 ccaaaactaa tattgctttt cactgtacca gaaaacagca ctcccttttg cggtacatag
     3721 ccaatttggc ggtagagatc ttcaagtttt aaatctcgaa tatcaactcc gtctattaaa
     3781 atttgacctg cagtgacatc ataaaatctc gggacaagat tgataagagt tgttttccca
     3841 cttccagtac tgccaattaa ggcagtagtt tgaccaggtt ctgcagtaaa gttaatagcg
     3901 gttaaaactg gcaaatcagc atcggggtaa gtgaaagtca catccctaaa ttcaatgcgt
     3961 cctttccctt gagcattggg cttaattggg gtttctgggt ctttgatcag cggttcggtt
     4021 tccaacaatt caacaattct tttacctgat actgcggctc ttggcaccat aataaacact
     4081 attgaaatca ttaaaaacga catgattact tgcatcgtat attggataaa tgccagcatt
     4141 tcgccaattt gtaaagaacc actgtcaatt aggtgtgccc caaaccaaac aatagcaata
     4201 acagcaaagt tgagcactaa attcatcgtt ggttgaagaa tagacattaa tctattgaca
     4261 aacaaattag ctcgcgttaa ttcttgatta gttttctcaa atttagcttc ctctctggat
     4321 tcattattaa aagctctaat aactttcaac ccagataggt tctctcgcgt caccagattg
     4381 agtttatcta ttaactcttg aactaattta aacttgggca aaccaaaaat gaagagcgcc
     4441 aaaatcatgg ttagcaataa tagagtagcc agagcgatga tccaacttat cgagggagca
     4501 gtcgccaggg ctttttgcag cgccccaaca gccataattg gagccatcaa aactattcga
     4561 aaaatcatgg taagagtcat ttgcatttgt tggacatcat tggtcgatct agtaatcaga
     4621 gaagctgtag aaaacttatt gaactcagaa acagaaaaac tttccacctt agtaaacacc
     4681 tcttgtctaa tatctcgcgc aaaaccagta gcaattctag aggccaaaaa accaactagg
     4741 acagcacaaa ttgccccaag caatgttaca accaacatta accaaccatg gtgataaata
     4801 atatcaacat tttttaaaag aatgccctcg ttaacaatct tggccatgta ttctggtaat
     4861 ttgagattag ccattacctg aaagtaggta actacgatca gggctagaat taaccaaata
     4921 tatggtttaa gaaattttat taatttgatc atatgaatac ttcaagaaac caagcggttc
     4981 tggaaaatgc aaaactctgt caactggttc cgttgtagtg tatattaata actaatattt
     5041 tgtaaatatg ctgaaaaaaa tcttattcta tttagtctat tttttaagcc ccgcggtact
     5101 ggtggcggtc atttattttt ctaacccaag ttattttgct gggtcacgct tcgatttgcc
     5161 taacagtgct aattcactaa acattagggg gtaatcaaca tcatgaatat ctattttaaa
     5221 agttggttca ccagcttgct tcatttcttc cagtaaagaa acaatggctt gtctaacttg
     5281 aggtagtact aactcctccg aacctcgata acgaatgaca acatcaatgt cgctatcggc
     5341 accagctcga cccagagcta ctgaaccaac aacctgaaac ttaatttctt gttgtaagtg
     5401 cttgggcaat tttgttatca atctggtttc aaaagcagta aaccaagcct gacgtttctt
     5461 agactgttcg gggctgtcag ggtcttccgg cctcattttg ccaatttcat ttctataaaa
     5521 tgaactcatt agtatttaat ttttgaatat ttttctaatg ctcagccgca taatccggat
     5581 cgtttaacgc atcttgttta gtgggatgaa ttttaccctc gggatgttca gctggagaat
     5641 aaacagtgta aagttgtagt gtttctgtat tagaagcatt gatcacattg tgtctttgtc
     5701 cggcgggaac gacaatagct acgccgtcag acaaatcata agtgctttca ccaatgatcg
     5761 cttgcccaac tcctttttcc acccgaataa attgatcgtg ttcctcatga gtttccatcc
     5821 caatatcatc tcctggggca atagacatca ctactaattg gctgtgagca gcagtgtaaa
     5881 gtacctctcg gaaattctga ttgtttaaag ttttctcttc taaattgatg ctgtagcctg
     5941 acatattatc ctttttgtga attaataaaa ttactctttc ttatagtgta gcatataaaa
     6001 caattaagta attaaaaatt ttaaaccaag ctctgtccag tcatttcttt tggttgaggt
     6061 agatccaata atttaagaac tgtcggagct atatctttca tcatcccttc atttctattt
     6121 aattgaccat cattgagtaa cacaaatggt acaggattca tactatgttc agtaactact
     6181 tctccttttt cgttgagcat ttcttcacaa ttgccgtgat ccgctgtgat tattaagaaa
     6241 tagttgttgg tttcagctgc ttctttaatt ttagctaggg ctaaatcaac tgtttcagcg
     6301 gccttaattg ctgcttgaat atctccagta tgacccacca tatctccatt acaaatgtta
     6361 gcaaaaatga cagaatgttc acctcgattc atggtttcga caattttatc agcgattgct
     6421 aaagctttca tttctggctt ttctgcatga ctctcaacct tattactatc aaacataaag
     6481 cgctcttcca agggataggg ctcttcacgc ttgcagttta aaaagaaagt aagatgagca
     6541 aatttttccg tttcagtcac cctgaactgt ttaatgtggt tttccgctaa tatctctccc
     6601 aaagtgcgct caatcttttc tggaccaaaa gcgacagcta ctgggtactg atcactataa
     6661 agagtcatgg tgacaaagaa aatgttttca ggaccttttt ctaaaaagcg ctcggttaat
     6721 tgacggggtc ggtcattgcg aaaattagca aaaatgacac tatcaccact tttaaccttg
     6781 gcctcttctt catctctgac tttaatggtg caggggacaa taaattcatc tcccttgtta
     6841 tttttatatg agtctgcaat ggcttgttgg gcactggcaa attggggtgc ttggcggtct
     6901 ttgatagcac tataagctaa atcagtgcgc tcccaattat gatcacgatc catagcatag
     6961 taacgaccag aaatgctggc caactcggct cctgtttctt ggcaaatttt tgctaacttc
     7021 tcaaaactgg ctaaacaact tcttggagct gtatcgcgac catcagtcat cgcgtgaaca
     7081 taaattttct ttaccccaaa atccgccgct tctttaataa gtccgaaaat atgcttttcg
     7141 tgagaatgca ctccgcccgg actcaacaaa cccataatat gcagcgatga tgagttattt
     7201 tttgccctgg taaaagcttc tttgatcgct tcagcttgat gcaatgtttt ttcgtcaacg
     7261 gccttattga gcttaactaa gtcttgataa atgacacgac ccgaaccaat ggccgcgtga
     7321 ttgatttcac tattacccat ctggccagct ggtaaaccaa ctgcttcacc tgaacaagcc
     7381 aatagtgctc gtggactgtc agcccaaatt ttgtcccaag taggggtatt ggctaaacta
     7441 atagcattgc ccgggtaatc cttgtagtaa ccccaaccat caagtatgat cagagccaat
     7501 tttttctgag aatctttaga catacctcct ttaaaataat taatttaaga aagaggggtt
     7561 tttcacccct ctctataagt caactatttg tgaaaaggaa atttggccat ggttgcctta
     7621 tctcctaatt ctcgttcaat catcattagt cgattatatt tagcaatccg ttcactgcga
     7681 cacatggaac cagttttgat ttgaccggta ccagcaccaa ctacaaagtc agcaataaaa
     7741 gtgtcttcag tttccccact gcgatgggaa accacacaag tccaaccagc acttcgagct
     7801 aatttaattg cttggattgt ttcactgacg ctaccaattt ggttaagttt aattagcgcc
     7861 gaattagccg ctttttgatc gatggctttt tgaattcgct ctgggttagt aactaatagg
     7921 tcatcaccca taatctgaat ttttttaccc agagttttag ttaattgcgc aaaaccagcc
     7981 caatcatctt cggccaaccc atcttcaatc gacactattg gatattcttc aacccatttt
     8041 gcatattttt taatcatgtc actactactc agttgcttat tttcagtttt gagatcgtaa
     8101 taactatttt ctccctcatc cttataaaac tcggtcgctg ccggatccaa ggcaataaac
     8161 acttcttcac caggcttata accagccatt tcaattgctt gaacaatcaa ttgtagaggc
     8221 gcttcattag ttccaagtga gggagcgtaa ccaccttcat caccaacggt tttttgccaa
     8281 ccctttttct caataatttt acctaaatgc tgaaaaattt ctgcattcca gcgcactgct
     8341 tccgcgatgc tttgagctgc tagagggaag atcataaact cttgcatgtc ggcaccacca
     8401 atggcgtgct tgccaccgtt gagtacgtta gacatgggaa taggcatgat aaacttgtca
     8461 gtgctattac caaataattg agcgatgtaa gcgtaaagtg gcattttgct agcgatggct
     8521 cctgctttga cacaagccat tgataccgcc aaaatggcat tggcccctaa gttttgctta
     8581 ccttgagtac catcaagatc gagcattatt tgatcaattt tttcttgctc tgtcacctgc
     8641 tgaccaatta tttttggagc aattacctca ttgacattag ccacagcctt gagtacacct
     8701 ttgcctaagt aacgacccgc atcgccatca cgtaattcta aagcttcata agcaccagtt
     8761 gatgctcccg atggcacggc cgaaaatact tcggtgccat cttcaagctt gagatttact
     8821 gctacggttg gattacctct ggaatctaaa atttcctgag ctttaattga tttgataatc
     8881 atgagtccct ttctatcttt tttaatatcg aggtgcgggc gggattcgaa cccgcgcacg
     8941 gaggttttgc agacctcgcc attggaccac tctggtaccg caccaaattg attgagctat
     9001 aagccaaaca aattttacat agctatttta atctaattac aatgaatgtg aaggggagag
     9061 gtaatatggt gttagaataa gagcacttca atgtcaaaaa aatattttat taaaacttat
     9121 ggttgtcagg ctaacaaaag cgatgcagaa cgcatggctg ctctttttga agaagagggg
     9181 ttaattgcca gtgattcgtg gcaagactgt gataagttgg ccgttgtgac ttgtagtgtc
     9241 cgcgaaagag cagaacaaag agttagagct tttttattaa aagtggccaa attttatcaa
     9301 gacaaaaagc taactaaacc ggagattatt ttttctggtt gcatgatcca tcatggtcaa
     9361 gaacatttga aaaaaatctt accaatgatt gaccagattg tgccgactaa tcagatggct
     9421 tttttaaaaa aagctaaacg ccaagacaag ttgcatgctt ttgtccctat ttcagtcggg
     9481 tgtaattcat attgtactta ctgcatcgtc ccttatgctc ggggtaggga aaaatcacgt
     9541 ccatttagcg acatcatgga agaagttaat ggcttagtaa aagaaggtta tcaagaaatt
//
LOCUS       DAOSEW010000020         9089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_6505_length_9089_cov_4.261235, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000020 DAOSEW010000000
VERSION     DAOSEW010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 9089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9089
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6505_length_9089_cov_4.261235"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(364..1410)
                     /gene="recA"
                     /locus_tag="PLJ02_01230"
     CDS             complement(364..1410)
                     /gene="recA"
                     /locus_tag="PLJ02_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOI05073.1"
                     /translation="MVKKSKDKDIATVAQDSKTAKSQAVAIAMSQIEKEFGKGSIMKL
                     GESLKDMPKVPVVSTGSLSLNIALGIGGYPKGRIVEIYGPEASGKTTLCLHAIAEVQK
                     QGGIAAFIDVEHALDPVRAEKIGVNLEEVLISQPDYGEQALEIAETLIRSGGVDIIVL
                     DSVAALVPKSEIEGEMGDHQMGTQARLMSQAMRKLTAALDKTKTLMIFTNQIRMKIGV
                     MFGSPETTTGGNALKFYASQRLDIRKIGNIQEGDEIVGTRHRVKVKKNKIAPPFKVAE
                     FDMDEAGICLAGDVLDLAVDRNIIEKSGSFYKYNNEILAQGREATKDKLKNNHGLLKE
                     IEKKIWQDLEDNKK"
     gene            1574..1647
                     /locus_tag="PLJ02_01235"
     tRNA            1574..1647
                     /locus_tag="PLJ02_01235"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1608..1610,aa:Pro,seq:ggg)
     gene            1735..2199
                     /gene="bcp"
                     /locus_tag="PLJ02_01240"
     CDS             1735..2199
                     /gene="bcp"
                     /locus_tag="PLJ02_01240"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HOI05074.1"
                     /translation="MLKENDQAPLDVKVINENGQETSLKEFLGRKILLYFYPKDNTPG
                     CTVEACNFRDANQEISQKGVQIIGVSKDSQTSHEKFKSNFSLNFPLWSDPEQKLIKAF
                     GVWGEKKMMGKTYFGTKRSTFLIDEKGKIIKVWPNVKAKQHSQEVLNYLEQL"
     gene            complement(2254..2952)
                     /locus_tag="PLJ02_01245"
     CDS             complement(2254..2952)
                     /locus_tag="PLJ02_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05075.1"
                     /translation="MQENSPDSLPPTETKNKTFVLVSAILLLAIAVIIAVFFNKNQAN
                     QANQDVKQQLEQPEPTTTEPTTVIWEGEVTTDAQQSEVEGTLLFSDLIAQKEGSYHCQ
                     IDNEDGYYVYYLDDQRMAVEVKTPTVHSKTIVTEDFTYTWNVAEKVGTKMANEEEILA
                     GNPDQEAEAVGEEMLAETPEENLLPVEDNEAFDPANFVCEPWVVEESVFTPPSDVIFQ
                     DPSQFQEQTTNVVQ"
     gene            complement(3022..3648)
                     /locus_tag="PLJ02_01250"
     CDS             complement(3022..3648)
                     /locus_tag="PLJ02_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05076.1"
                     /translation="MPEQSQTAPTKNQAAKPKEAEAANQRVNLPKSAELEKSKVAPGY
                     YRPIPEETLVTWEAPARPFKKRNKQFFSTVLIIAILIGLILFFAGQVLPVALVISVVF
                     LVYVTAMIPPQNVSVKLTNYGFYIDKEAYAWYEMGRFWFEKKQGFDTLQIELGRFPNR
                     LSLVLIDGVTPSKEDLESVLSEVLLKEKPKPTTFEKAAKWLSEKIPLD"
     gene            3780..3862
                     /locus_tag="PLJ02_01255"
     tRNA            3780..3862
                     /locus_tag="PLJ02_01255"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3814..3816,aa:Ser,seq:tga)
     gene            4064..5062
                     /locus_tag="PLJ02_01260"
     CDS             4064..5062
                     /locus_tag="PLJ02_01260"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOI05077.1"
                     /translation="MGMDNLKKNVAIVGLGKQSINDLIPMVQNHPNCILSAVCDISQE
                     LIDKVLTSLSNPSEKKISAFLDYKKLFESQRNGDLKIDFVVISVPHFLHFDICKEAIK
                     SGVPIFKEKPFALNLDEAKRLKLLSQESKVPVYTVTKRQFYPSYNKGQELLKAGTIGT
                     PYMYSVRHFIAHGNLYEGWRSKLETAGGGVFMDMGYHLLDVIVRYFGSIEKLSMAGSS
                     VAKPGFAYEVEDAATLHFVHNQQLHGLFEIGALTGPKEERIEVRGSDGQLIINEKSVI
                     HSDNYGKVLKKYDLPADGLKANIQALNAFLSGEQSIIRQNLEHNLELMKIMDKVYH"
     gene            5073..5804
                     /locus_tag="PLJ02_01265"
     CDS             5073..5804
                     /locus_tag="PLJ02_01265"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOI05078.1"
                     /translation="MKKAIVTGASGGLGIEICKKLVANNFKLIVIDLADKETIENSLS
                     EVNDGIELILDKVDFSKTDELQSSLDSCLPDENLTVLVNNAGLSIGGGISDATLAEWE
                     LMIKVNLTAPFILSQAFINNAKKFNIQGSIINISSMVGIIGAKKPGYAASKAGLLGLT
                     KSIAMQSGPKIRCNAIYPGAMDTPMTADWDEHTRHKIIKNTPLQRIAQPAEIANIVGF
                     LADDNESSFITGAVINATGGQYLGQ"
     gene            5806..6420
                     /locus_tag="PLJ02_01270"
     CDS             5806..6420
                     /locus_tag="PLJ02_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05079.1"
                     /translation="MLLNSYHHIWFCGLAASGKTTALKTISDLDLGVNFYNDSSEILD
                     FIKLDTQEKFHVKPTPTSFILTSSKPVKYSIEQLIKKVSTNSGKNIIELSRGIDLEAV
                     IDFTYKDFFSQLPENIKNESLIVYLHSSFEERISRNNKRSAPSENHTVFESFHCPQEA
                     MKRFFSKDDFFDEINSVDLDALIIANTKSIDELKKKIISLFKEQ"
     gene            6423..6989
                     /locus_tag="PLJ02_01275"
     CDS             6423..6989
                     /locus_tag="PLJ02_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOI05080.1"
                     /translation="MIGKKELLNFLKSYKTDFPNEKNDVAKFIDLINAHQDIFFRECE
                     VAHITGSALVINLTTRRILLHKHKKLNRWLQFGGHADGELDIKKVALKEATEESGLSD
                     LNFLFADPIDLDLQLIPARNEEPEHYHLDFRYLMETSATSVPKIEANESQELNFFSID
                     DHSLENIKIDPSLHRLISKTQKLINNIN"
     gene            complement(7006..7833)
                     /gene="pyrH"
                     /locus_tag="PLJ02_01280"
     CDS             complement(7006..7833)
                     /gene="pyrH"
                     /locus_tag="PLJ02_01280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOI05081.1"
                     /translation="MLIDIPNKNFSKISFFISGLTSKCDRILISINKKVPCMPQYELR
                     EDNKKAVISLGGSLINPGEIAVDFLKKFKVFIEKRVAEGWQFIVITGGGAPARQYIEG
                     ASAIMGKEIDKNDMDWLGIHATRFNAHLMRTIFRKIAQPVIITDPEKDFIDPEKAIVI
                     GGGSEPGWSTDYVSTAVAARINAPYVINLSNIKQAYTEDPKKNPNVKPIDNIKWVDFC
                     KIVGDTWEPGLNTPFDPIASKLAKEKNITVLIVEGTNLENLEKLFKSEEFVGTVISN"
     gene            8089..8478
                     /locus_tag="PLJ02_01285"
     CDS             8089..8478
                     /locus_tag="PLJ02_01285"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOI05082.1"
                     /translation="MNNESDYKKVTVPTAISAIVPVFNEEQTVALVIEALLHSNVIDE
                     VICVNDGSTDHSLAILKKYQDQIKLINLKKNQGKGYALAEGIKSAKNEIVAFIDADLT
                     NLADEHIKALVKPILTGKFKAVLGLSA"
     gene            8676..8936
                     /locus_tag="PLJ02_01290"
     CDS             8676..8936
                     /locus_tag="PLJ02_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05083.1"
                     /translation="MPKAKNKKATKKAKSTPQSESLLKKEIFSEQQAAFYKKHPHAKT
                     LISILILLFLVLMSLYLNEYGNAWISELRLFFVSQGATQAAF"
BASE COUNT         3002 a         1677 c         1556 g         2854 t
ORIGIN      
        1 cttagcttcg tcttcaactg aaaacagcgt ggataaatta ctaaaaaatg acataaatat
       61 cctttactta taaacaaacc gctgataaaa ccttggagaa agggttaaca cccctgagta
      121 ttattatcga aaaattgtaa gaaaaatgaa aatgctgggt atcttacttc ttgcataaat
      181 aataaattga tttcgatgtt gtaagttctt taataatacc tgatccaaaa gtcttattga
      241 tcagcttgtg tatttaataa tgtttatatt aataactagt ggggacatta tatgtcaata
      301 aagcaacaaa gtcaatttct tggctactag agtaaagaat aaaaagaact atgtaccaat
      361 cttttatttc ttattatctt ctaaatcttg ccaaattttc ttttcgattt ctttgagcaa
      421 accatggttg ttttttaatt tatccttagt tgcctcacga ccttgagcta atatttcgtt
      481 attatattta taaaaactac ctgatttttc aataatgttg cgatcaactg ccaaatcaag
      541 cacatcgcca gccaaacaaa tgccagcttc atccatgtca aattcagcga ccttaaaagg
      601 aggagcaatt ttatttttct tgactttaac tcgatgacga gtaccgacaa tttcatctcc
      661 ctcttgaata ttaccgattt ttctaatatc tagccgttgt gaagcataaa acttgagcgc
      721 atttccaccg gtggtagttt ctggactgcc aaacatcacc ccaattttca tgcgaatttg
      781 gttagtaaaa atcattaagg ttttagtttt atcgagtgca gcagttaatt tacgcatggc
      841 ttgagacatc aaacgagctt gggtgcccat ttggtgatcg cccatctcgc cttcaatttc
      901 tgacttggga actaaagcag ctaccgaatc taaaacaatg atatccaccc ctccagaacg
      961 aattaaagtt tcagcaattt ctaaagcttg ttcaccataa tctggttgag aaattaacac
     1021 ttcttctaga ttgacaccaa ttttttcggc acgaactgga tctaacgcat gctcaacatc
     1081 aataaaggca gctataccac cttgcttctg tacttcagcg atagcatgta gacagagtgt
     1141 agttttgcca gaagcttctg gaccataaat ttccacaatg cgacctttgg gataaccacc
     1201 aataccgaga gcgatattaa gagacaaaga accagtagat accacaggca ctttaggcat
     1261 atctttgaga gattcaccta atttcatgat tgaaccctta ccaaattctt tctcgatttg
     1321 cgacatcgca atagctaccg cttgtgattt agctgttttg ctatcttgag caactgtagc
     1381 aatatcttta tctttactct ttttgaccat tttagcttct ccttttttat tactgctctt
     1441 acttataaca ctatccatag tttcgtgcaa attacgcttt tttactcagt tatttaaaag
     1501 aatattacat aaaatagaat aatagtcaaa gtttacataa aagcttacaa gcagtaaaat
     1561 caaggctaag aaacggagag tagttcagat ggctagaacg tacgcatggg gtgcgtaagg
     1621 tcgcaggttc gagtcctgtc tctccgacat ttactccatc cccaacaatt taattaagca
     1681 agtttgggca ataatttgac tttattcagc cgtccgtcat attatacgag tactatgctt
     1741 aaagaaaatg atcaagcccc cttagatgtt aaagtcatta atgaaaatgg acaagaaact
     1801 agtctaaagg aatttttagg ccgaaaaatt ttactctatt tttatcccaa agataacact
     1861 cctggttgca ccgtcgaagc ctgtaatttc cgcgatgcta atcaagaaat aagtcaaaaa
     1921 ggcgttcaga tcattggagt cagcaaagat tcacagacta gtcatgaaaa atttaaaagt
     1981 aatttctcat taaattttcc cctctggtca gaccccgaac aaaaattaat taaagctttt
     2041 ggggtgtggg gtgaaaaaaa gatgatgggc aaaacttatt ttggcaccaa acgcagtact
     2101 tttttgattg atgaaaaggg taaaatcatt aaagtctggc ctaatgtgaa agccaaacaa
     2161 catagtcaag aagtacttaa ttaccttgaa caactataaa aaaacaatcc acaagctcaa
     2221 aaatttttaa ctataaatta gtattattac ggattattga acaacattcg tcgtttgttc
     2281 ttgaaattga cttggatctt gaaatatcac gtcactcggc ggagtaaaaa ccgattcttc
     2341 aactacccaa ggttcacaaa caaaattagc tggatcaaaa gcttcattgt cctcgaccgg
     2401 taataaattt tcttcaggtg tttctgccag catttcctca ccgactgctt ccgcctcttg
     2461 gtctggatta cctgccaaga tctcctcttc attagccatt ttagtcccaa ctttttccgc
     2521 tacattccaa gtataagtga aatcttcagt aacgatcgtt ttactgtgaa cagtgggtgt
     2581 cttgacctca acagccatcc gctgatcatc aagataataa acatagtagc catcttcatt
     2641 atcaatctga caatgatagc taccctcttt ttgagcaatt aagtcactaa atagtaaagt
     2701 accctctact tcactttgct gagcatctgt agtcacttct ccttcccaaa tcaccgttgt
     2761 tggctctgtc gttgtgggct ctggctgttc tagctgttgc ttcacatctt gatttgcctg
     2821 attcgcttga tttttattaa aaaaaacagc aataatcacg gcaatggcta ataataaaat
     2881 agcactaacc agcacaaaag ttttattttt tgtttcagtc gggggcaagc tgtcggggga
     2941 attttcttgc ataattggct caattatagc acaaacccca tcaaacaaaa ttaaaatagt
     3001 tagcttgatt tatcaattta attaatctaa aggaattttt tcactcaacc actttgcagc
     3061 tttttcaaaa gtggtgggct tgggtttttc ttttaataaa acttcagata aaaccgactc
     3121 taaatcttcc ttactgggcg tgactccatc aattaaaacc aaacttaaac gattggggaa
     3181 acgtcctaat tcaatttgca aagtatcaaa accctgtttt ttttcaaacc aaaaacgccc
     3241 catttcatac caagcgtagg cttccttgtc gatataaaaa ccataattgg tcaatttaac
     3301 tgaaacgttt tgtggtggaa tcattgccgt cacataaact aaaaaaacca cagaaattac
     3361 caaggcgacc ggcaaaactt gaccagcaaa aaataagatc aagcctatta aaattgcaat
     3421 aataagaacc gtactaaaaa attgtttatt gcgcttttta aacggccgag caggagcctc
     3481 ccatgtcacc aaggtttctt cgggaattgg ccgataataa ccaggtgcta ccttcgattt
     3541 ctcgagttct gcagatttag gtaaattgac cctttgattg gcagcctctg cctctttggg
     3601 tttggcagct tgatttttag ttggggcagt ttggctttgt tcgggcatca ttaaaaagta
     3661 tagcattttt tcttgacaat ggggagaatt aacccttttt aagcaaaaac ttgttataat
     3721 tggggggttc gcaagtgctt ttaataagtt ttagctaaaa aacggcttac ttaacactag
     3781 gaggtgtcgc atagcggtca attgcactgg tcttgaaaac cagagcctca cggcttcaca
     3841 ggttcgaatc ctgtcacctc cgccgtcgag cggactcgaa cctattgttg ttttgaaagt
     3901 cggcaacacg taattaatcc ccgaagaaaa cgctgttaga taatcaattt ttatcaagca
     3961 ggttttgcca taaaaatatt ctagtttaat tgactttcta cctacaaaat aatagaatat
     4021 atctttctta tttttagctg atttaacaaa taatgtggat aatatgggaa tggataattt
     4081 aaagaaaaat gtggctattg ttggattggg caaacaatct ataaacgact taattcccat
     4141 ggtccaaaac cacccaaact gcattctgtc agctgtttgt gacattagtc aagaattaat
     4201 cgacaaggtt ttaacatctc tttcaaaccc aagtgaaaag aaaattagtg cttttcttga
     4261 ttataaaaag ctttttgagt ctcaaagaaa tggtgactta aaaattgatt ttgttgttat
     4321 ttctgttcct cactttttac atttcgacat ctgcaaggaa gcaattaaat cgggcgttcc
     4381 aatttttaaa gaaaaaccct ttgctttaaa tctggatgag gctaaacgtt tgaaattatt
     4441 atctcaagaa agtaaggttc cagtttacac cgttacaaaa cgtcagtttt atccttctta
     4501 taataagggg caagaactct taaaagctgg aacaattggc acaccttaca tgtatagcgt
     4561 tagacatttt attgctcacg gcaatctata tgaaggttgg agaagcaaac tggaaactgc
     4621 tggtggcggg gttttcatgg atatgggcta ccatttactt gacgttattg ttagatattt
     4681 tggtagtata gaaaaattat caatggcagg ctcaagtgtg gctaaacctg gatttgctta
     4741 tgaagttgaa gatgcggcta ctctgcattt tgtacacaat cagcagctgc atggtctttt
     4801 tgaaattgga gcattaaccg gaccaaaaga agaaagaatt gaagttcgag gaagtgacgg
     4861 ccaacttatc atcaacgaga agtcggttat tcattctgat aactatggaa aagtgctaaa
     4921 aaaatatgat ttgccagctg acggattaaa agccaacatc caagcgctta acgctttttt
     4981 aagtggcgag caatcgatta ttcgccaaaa cctggaacac aatttagaat taatgaaaat
     5041 tatggacaag gtttatcact aggaaaacaa atatgaaaaa agcaattgtt actggagctt
     5101 caggaggatt aggcattgaa atctgcaaaa agctggtcgc taacaatttt aaactgatcg
     5161 tcattgattt ggctgataag gaaactattg aaaattcatt aagtgaagtg aatgatggta
     5221 ttgagctaat tttagacaaa gtggactttt caaaaacaga tgagcttcag tcaagcctag
     5281 atagttgttt acctgatgag aatttaaccg tcctagtaaa taatgccgga ctcagcatcg
     5341 gcggtggaat tagtgatgca actttggccg aatgggaatt aatgattaag gtaaatttga
     5401 cagctccttt tattttaagt caggccttta ttaataatgc aaaaaaattc aatattcagg
     5461 gttcaatcat taacatctct tcgatggtcg gtattattgg ggcaaaaaaa ccaggctatg
     5521 ctgcctcaaa agctggatta ctaggtttaa ccaaatcaat tgccatgcaa tcaggaccaa
     5581 aaattagatg taatgctatt tatccaggag caatggatac gccaatgact gctgattggg
     5641 atgaacatac tagacacaag attataaaga acacgcctct ccagcgaatt gctcaaccag
     5701 ctgaaatagc caacatcgtt ggttttttgg ctgatgacaa tgagtctagt tttataactg
     5761 gggcagtaat taatgccact ggaggtcaat acttgggcca ataagatgtt gctaaattct
     5821 taccatcata tttggttttg tgggctggca gcatctggga agactacggc tttaaaaact
     5881 attagcgatc ttgatttagg ggtaaatttt tataatgatt cgtctgagat tttagatttt
     5941 attaaattag acactcaaga aaaatttcat gtcaaaccaa cgcctactag ttttatccta
     6001 actagttcta aacccgtaaa atactctatc gagcaattga taaaaaaggt ttcaactaat
     6061 tctggaaaaa acataatcga attatctcga ggaattgacc ttgaagcagt gatagacttt
     6121 acttataaag attttttttc tcaattacct gaaaatatta aaaacgaatc cttaatcgtt
     6181 tatttacaca gttcatttga agaaagaatc agtagaaata ataaaagatc ggctccaagt
     6241 gaaaatcata ctgtttttga atcgtttcat tgccctcaag aagcaatgaa aagatttttc
     6301 tcaaaagatg acttttttga cgagatcaac tcagttgacc ttgatgcctt aataattgca
     6361 aacactaaat cgattgacga attaaagaaa aaaataattt cactctttaa ggaacaataa
     6421 tcatgattgg caaaaaagag ctattaaatt ttttgaaatc atataaaact gatttcccca
     6481 atgaaaaaaa tgatgttgct aaatttatag acttaataaa tgctcatcaa gatatttttt
     6541 ttagagaatg cgaggttgca catatcacag gatcagcttt agtaattaat ctaacaacaa
     6601 gaagaatttt gcttcataag cataaaaaac taaatcgttg gttgcagttt ggcggtcatg
     6661 ccgatggaga attggatatc aaaaaagtgg ctctcaaaga ggcaacggaa gagtccggtt
     6721 taagtgattt gaatttttta tttgctgatc ccatcgatct tgatctacaa ctaataccgg
     6781 ctaggaatga ggaaccagag cattaccacc tagattttag atatctaatg gaaacttcag
     6841 cgacaagtgt gccaaaaatt gaagcaaacg aatctcagga attaaatttt ttttcaattg
     6901 atgaccattc tctagaaaat attaaaatag atccttcttt gcacaggcta atcagcaaaa
     6961 cccagaaatt aatcaataat attaattaaa acagtatcgt ttcacttagt ttgaaattac
     7021 agttcctaca aattcttcag atttgaaaag tttttctaga ttttctagat tagttccttc
     7081 aacgatcaac actgttatat ttttttcctt agccaactta gatgcaattg gatcaaaagg
     7141 agtatttagt cctggctccc aagtatcgcc aacaatcttg caaaaatcga cccatttgat
     7201 gttgtcaatc ggcttaacgt ttggattctt tttcggatcc tcggtgtatg cttgcttaat
     7261 attggataaa ttaataacgt aaggagcatt aattctggcc gcaaccgcag tagatacata
     7321 atctgtagac caacctggct cggagccgcc accaattacg atagcttttt ctggatctat
     7381 aaaatctttt tcaggatcag taataatcac tggttgagca atttttcgaa aaatcgttct
     7441 catcaaatga gcgttaaatc gtgttgcatg aatgcccaac caatccatgt catttttatc
     7501 aatttctttt cccatgatgg cagaggcgcc ctctatatac tgacgtgcag gagcaccacc
     7561 tccagtgata actatgaatt gccacccctc agccactctt ttttcaataa aaactttgaa
     7621 tttttttaaa aaatcgaccg ctatttctcc tggattgata agcgaacctc ctagtgaaat
     7681 aaccgctttt ttattgtctt cacgcagttc atattgtggc atacatggaa ctttcttgtt
     7741 aattgatata agaattctat cacacttact ggttaaacca gaaataaaaa aagaaatttt
     7801 gctgaaattt ttatttggta tatcaattaa caagaaaata ttaaattaat taaacctgtc
     7861 tctttgtcgc caactgcccg caagcagcat cgatatcttc acccatattc acacccaaat
     7921 ggtatggtgg attcacgtct gttttaaaac cggagcctca cggcttaaca ggttcgaatc
     7981 atgtcagtta tgcaacaatt atgagatgtt tattgcttta accaattttt cgtttttttg
     8041 tacactatat tgtaactagc tatcagtaag gatattataa taatcttcat gaacaatgag
     8101 agtgattaca aaaaagtaac agtgccaact gcaatttcag ccatcgttcc ggtatttaac
     8161 gaagaacaaa ctgttgccct agttatcgaa gctttgctac acagtaatgt gatcgacgag
     8221 gttatttgtg taaatgatgg ttcaacagat cacagtctag ctattttaaa aaaatatcag
     8281 gatcaaatca aactaattaa tttgaaaaaa aatcaaggaa aaggttatgc cttagccgaa
     8341 ggaataaaaa gtgctaaaaa tgaaatagtt gcttttattg atgctgattt aaccaatctt
     8401 gctgatgaac acatcaaagc tcttgttaaa ccaatcttaa cgggtaaatt taaagctgtc
     8461 ttaggattgt cagcttagtc aatgtgctta acttcaaaga actaaatcga aaaattaacc
     8521 acatcaaaaa tatgcaagtt aagcaatttc tcgagaaata tgttttaagt tacgttaaaa
     8581 aactctagag ctaacgtcgt ttatgatgtt caacatcagc tttttgggat agaatgactt
     8641 taggtattaa tcatagtaaa aaatctgact tacttatgcc aaaagccaaa aacaaaaaag
     8701 ccactaaaaa agccaaatcc accccacaat cagaatcact tttgaagaaa gaaatctttt
     8761 cagaacaaca ggctgcattt tataaaaagc atccacatgc caaaaccctc atcagtatcc
     8821 taattctttt attcttagtt ttaatgagtt tgtatcttaa tgaatatggc aatgcttgga
     8881 tcagtgaatt aaggttgttt ttcgtgtcac agggagccac tcaagctgca ttttaattaa
     8941 ataaagagtt gtcaaagaat taattgctga atcatcaagc aactgtttag agttttctga
     9001 ccttttttct cagcaattca aatgttttaa ccaaaaagta attactagaa aagggaaaat
     9061 cttgggcagg taaataagcc acttcttta
//
LOCUS       DAOSEW010000021         8581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_7021_length_8581_cov_4.579404, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000021 DAOSEW010000000
VERSION     DAOSEW010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 8581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8581
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7021_length_8581_cov_4.579404"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PLJ02_01295"
     CDS             complement(<1..382)
                     /locus_tag="PLJ02_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOI05084.1"
                     /translation="MQKTILLNWKANKNILEAEYWLNNFPKNLLDKKSDFKLIIAPPL
                     PLLSQMRRLIDHQQLPISLAVQDISAQKAGAYTGEVVAKNLEFLGVEMAIIGHSERRR
                     LFNENCQLVATKVQRALEADIGVVL"
     gene            449..2431
                     /locus_tag="PLJ02_01300"
     CDS             449..2431
                     /locus_tag="PLJ02_01300"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOI05085.1"
                     /translation="MFESQEILDQLNSEQRQAVLHNGGPAVVLAGAGSGKTRVLTSRV
                     AYLIKEGLAQPREILLLTFTNKAANEMKQRVLTYTGKQLMFSGTFHSLGVKILRLESQ
                     KHPQLTINQRVISAQFTIYDDQDQLQLLKNICQQENIDQQEFKLTVLQSLISQIKNQL
                     LSPIDYQNSANNDFDLKVAKIYKIYERRLKAENALDFDDLLILSYQLLKEHADIRQQY
                     QNQFKYVLIDEFQDTNKVQYLLSKILAKPQNDLFVVGDFSQSIYAWRGADYRNLRQLS
                     IDFPQLKEYRLEQNYRSTQNILDAATQVIQQNQLHPILQLWTDRHSDDEAKIQLYELS
                     NSELEAEKIADIIEKNYLDSLDQIAILYRTNAQSRAIEEAFIRHRLSYQIIGGVKFYQ
                     RKEIKDLLAYLRLAVNELDSPSLNRTIKLGKRRFRNFREWLKKVDEHILRNPALALQE
                     ILKTTDYLEKFDIKNPDDQNRLDNIEELINVASQFEDTLVFLENVALVQDGYLPEGKN
                     HQPSGGIKLMSLHSAKGLEFEIVFMIGMEEGLLPHSRSMFNEEELEEERRLCYVGITR
                     AKKQLIFTYARNRYQFGQQQQNLPSRFLSEIDKRLLTISRHFDETINDWQQRPNQKTK
                     RQSTRLSVKSKRLIIDDEQLDALLSGDLEIKDFLDD"
     gene            2484..3518
                     /locus_tag="PLJ02_01305"
     CDS             2484..3518
                     /locus_tag="PLJ02_01305"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HOI05086.1"
                     /translation="MLIRNIRPEEKDLYNRVVDHPIQSYEWGEFRRKTGLEVERIGFF
                     DAGQLKKAMTVTFHQLPLINKKVGYFPKGFKPDEEQLSTLQQLAQKHDAIFIKLEPAV
                     TKELDQNHQDHPSIKFLQNYQVETGRPLFTPFTFHLDLTLSEEELLAKMTSKTRYNTR
                     LAIKKGVTVVEDNSEQGLEDYLKILEETTKRQAFYAHSPDYFRQMWATLKDSNIMHIL
                     KASFEGQVLVVWILFVFNDVLYYPYGASSSQHREVMASNLMMWEAIRFGQKQQCQLFD
                     MWGALGPNPDPKDPWYGFHRFKRSYGGKLVEYIGTYDLVNNYPIYKIYNLADNWRWKF
                     LRFKKKFIKL"
     gene            3543..4592
                     /gene="gap"
                     /locus_tag="PLJ02_01310"
     CDS             3543..4592
                     /gene="gap"
                     /locus_tag="PLJ02_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOI05087.1"
                     /translation="MKNFAINGLGRIGRLAFRVWFAKHRQELDLKVINTSGSMDLEGW
                     VKLLKYDSTYGTFSDKITFEQLKKVEELKEGEIELGYIMVADHKILVTAERDPAKIPW
                     EKYGVYSVLEATGAFDKAKKAALHLKGGAKKVLLSAPGKEDPEGLISTGVISVNELDQ
                     TKNIFSNASCTTNCVAPIVKIIEDNFGIEKAILTTIHSYTSDQNLHDNSHKKDLRRAR
                     AAAINLVPTSTGAAKATADIIPSLKNKFDGLAIRVPTPTVSLSDITFVLSKASNLEEI
                     KQVFIEAAKGKYKGIVAVSQEPLVSSDFIGCEYSAVVDLPLIQLIDGNLLKVVAWYDN
                     EWGYSCRLMEQLSQI"
     gene            4632..5666
                     /locus_tag="PLJ02_01315"
     CDS             4632..5666
                     /locus_tag="PLJ02_01315"
                     /inference="COORDINATES: protein motif:HMM:NF017509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOI05088.1"
                     /translation="MAKSTSETKTKKATETNNSKSGTKTAKKTNSTQEAKPSLIAPND
                     KITITIPWSELKGPYDKALADLAKKMKIEGFRQGKVPLNIAEKQISLEKVAETMLQDI
                     LPKKYIEALQKAKKQPITAPEYHLISIKKGEDWVLEAHFAEAPSIDLKNYEKYIKAGK
                     QAAKEFIKKHNQQAEQKNPPKVTKTNTSDKDQQQKSEQISKLSPDQEQEIYYQHILRE
                     LVVNIRPSIAELLLRRETQAEFENFKEQLASYNISVEKYLEQRQIDLEQLGNEIAGTV
                     LNRLQIDFILAAIAKERQLKVDDQALKKALAEIKDDKLRDQIANHEQYLTSFKAQLLR
                     RQTIETLVKD"
     gene            5792..6448
                     /locus_tag="PLJ02_01320"
     CDS             5792..6448
                     /locus_tag="PLJ02_01320"
                     /inference="COORDINATES: protein motif:HMM:NF039817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5660 domain-containing protein"
                     /protein_id="HOI05089.1"
                     /translation="MNNLNQNISGQAARKQSTNQSGALARALLESEKNAGTYTPQNEP
                     PKPELDPFAQALSRAGGSFLSPLNEQSANEQADLLAQQKKEALRRQLHERINPVDMHE
                     IFAAQAEKNLKELEATRDELKMLAEEVKELDQEIDSALSREVVDPGITGIGFFNFFHK
                     LREWIGLLREQVSSAKTWMNQVSGKGKRGRGTAFDFSKSKNVHDAMNSERNFGMNMGG
                     "
     gene            complement(6461..6534)
                     /locus_tag="PLJ02_01325"
     tRNA            complement(6461..6534)
                     /locus_tag="PLJ02_01325"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6498..6500),aa:Val,seq:gac)
     gene            6710..7267
                     /gene="infC"
                     /locus_tag="PLJ02_01330"
     CDS             6710..7267
                     /gene="infC"
                     /locus_tag="PLJ02_01330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOI05090.1"
                     /translation="MIRKKYNKQRKDNLFVKANAHIHFPQVRVLTEHGEMVGIMSSRE
                     AFQQAMDEGKDLVLITEKTQPPVVKIIELSKFKYQLKKKEADNRKKSKIQDLKELRFS
                     PVIEEGDYQTRLKQLREFLTKGHKVKITLFFRGRQIAYKEAGYEVFARIIQDTEDLAK
                     VEVEPRLIGKKLQAQLAPLGKEDKK"
     gene            7276..7479
                     /gene="rpmI"
                     /locus_tag="PLJ02_01335"
     CDS             7276..7479
                     /gene="rpmI"
                     /locus_tag="PLJ02_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003970213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOI05091.1"
                     /translation="MKKIKVKTRKGAARRFHITGSGKVMRRIQNRRHLRRNKSKKAIR
                     NYRIPVEVKGPYAKKIRKMLGLN"
     gene            7497..7850
                     /gene="rplT"
                     /locus_tag="PLJ02_01340"
     CDS             7497..7850
                     /gene="rplT"
                     /locus_tag="PLJ02_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOI05092.1"
                     /translation="MPRTKTGVVRRRHHKKILKLNKGFRGANRRLYKRAKEASLHAGQ
                     YAYVGRKLRKRDMRKLWIIRINAGLKQINQQFNYSRFINNLKKAKIALNRKVLSELAV
                     SDFDTFKEIVVKSQK"
     gene            7996..>8581
                     /locus_tag="PLJ02_01345"
     CDS             7996..>8581
                     /locus_tag="PLJ02_01345"
                     /inference="COORDINATES: protein motif:HMM:NF013568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOI05093.1"
                     /translation="MSHYQDLVKQSQALLSQFQAERKQLNAEQLSIKYLGKKGLFNEL
                     AGQIKNLKRSEDKKEFGQILNQLKKDLETALADSQKQQELVHERLDLSLPGIKSNSGH
                     LHPVSQAIEEISQIFERIGFVRGDYPEVDWDHYVFAALNMPANHPARDEWETFFVDQP
                     ADPKLGKVVLTTHTSNGQVREMERVKGRLPIRMIN"
BASE COUNT         2999 a         1592 c         1641 g         2349 t
ORIGIN      
        1 acaacactac gccgatatca gcttctaacg ctctctggac tttggtcgcc actaattggc
       61 agttttcatt gaatagccgt cgtcgttcac tgtgaccaat aatggccatt tctacaccca
      121 aaaattctaa atttttggct accacctcac ccgtgtaggc cccagccttt tgggcactaa
      181 tgtcttgtac cgccaatgaa attggcaact gttgatgatc aattaagcga cgcatttgac
      241 ttaacagagg cagtggggga gcaataatta atttaaaatc gcttttcttg tccagtaaat
      301 ttttggggaa attatttaac caatattcag cttccaggat atttttatta gctttccaat
      361 ttaacagtat ggttttttgc acattttcat tttagccaaa agccattaac aaagcaattt
      421 aagccatttt tatgctagga taaaaatcat gtttgaaagt caggaaatcc tagaccagct
      481 caatagcgaa cagcgacaag cagttttaca taacggtgga ccagctgtgg ttttagctgg
      541 ggcgggaagt ggtaaaacaa gagtgttaac gagtcgcgta gcttatttaa tcaaagaagg
      601 attagcacaa ccaagagaaa ttttacttct aacttttacc aataaagcgg ctaatgaaat
      661 gaagcaacgg gttcttacct acacgggtaa acagctcatg ttctctggca cttttcacag
      721 cttaggagtg aaaattctgc gcttagaaag tcaaaaacat cctcaattaa ccatcaatca
      781 gcgagtaatt agtgctcaat ttactatcta tgatgatcaa gatcagttgc aattgctaaa
      841 aaatatctgc caacaagaaa acattgatca acaggagttc aaactcactg ttttgcaatc
      901 gctcatcagt caaattaaaa atcaattatt aagtccgatt gattatcaaa atagcgccaa
      961 caacgatttt gatctaaaag tcgccaaaat ctataaaatt tatgaaagaa gacttaaagc
     1021 agaaaacgcg ctggattttg atgatttgtt aatcttaagt taccaactcc tcaaagaaca
     1081 tgccgatatt cggcagcaat atcaaaatca gttcaaatat gtgttaatcg atgagtttca
     1141 ggatactaat aaagttcaat atttacttag caaaattttg gcaaaaccac aaaacgatct
     1201 tttcgtggtc ggggattttt cacaaagtat ttacgcttgg agaggcgctg attatcgtaa
     1261 tttgcgacaa ctgagcatcg actttccaca actcaaagag taccgtttgg aacaaaatta
     1321 tcgctccacc caaaatattt tagatgccgc aactcaagtc atccaacaaa accaactaca
     1381 tccaattttg caactgtgga ccgatcgcca tagtgacgat gaagcaaaaa ttcagctata
     1441 tgaattaagt aatagtgaac tggaagcaga aaaaattgcg gacattattg aaaaaaatta
     1501 tttagattca ctagatcaaa ttgccattct ttatcgaact aatgctcaat ctcgagcaat
     1561 tgaagaagct tttatccgtc atcgtttaag ttaccaaatc attggtggag tcaaatttta
     1621 ccagcgcaaa gagatcaaag atttgctggc ttatttgcgc ttagcggtca atgagcttga
     1681 tagtcccagt ttaaatcgaa ctatcaagct agggaaaagg cgtttcagaa attttcgcga
     1741 gtggctaaaa aaagtggatg aacatatttt gcgcaatcct gctttagctc tgcaagaaat
     1801 attaaaaacg acagattatc tagaaaaatt cgatatcaaa aatcctgatg atcaaaatcg
     1861 cctagacaat attgaagaat taattaatgt cgccagccaa tttgaggata ctttggtctt
     1921 tttagaaaat gtggctttag ttcaagatgg ttatctacca gaggggaaaa accatcagcc
     1981 aagtggagga attaaattaa tgagtcttca ttctgccaaa ggcttagaat ttgagattgt
     2041 atttatgatt ggcatggaag aaggcctatt gcctcatagt cgctcaatgt ttaacgaaga
     2101 agaattagaa gaagaaagac ggttatgtta tgtcggcatc acccgagcta aaaaacaatt
     2161 gatttttacc tacgcccgca atcgctatca atttgggcaa caacaacaaa atttacccag
     2221 tcgctttttg agtgaaattg ataaacgact cctcaccatt agtcggcatt tcgatgaaac
     2281 aatcaatgac tggcaacagc gaccaaatca aaaaactaaa cggcaatcta ctcgattgtc
     2341 cgtaaagagc aaacgactaa ttattgacga tgaacaatta gatgcgttgc tcagtggcga
     2401 tcttgaaatt aaagattttt tagatgacta aagagccggc tgacaactct gcttttttct
     2461 cttataatga ataatcgtaa tttatgctta tccgtaacat tagacctgaa gaaaaggatt
     2521 tatataatcg cgtcgtcgat catcctattc aatcatatga gtggggcgag tttcgccgaa
     2581 aaaccggttt agaagtagaa cgcattggtt tttttgatgc tggccaatta aaaaaagcca
     2641 tgactgtcac ttttcatcaa ttgcctttaa ttaataaaaa agttggttat tttcccaaag
     2701 ggtttaagcc agatgaggaa caattgagta cactccaaca gttggcgcaa aaacatgatg
     2761 caatttttat taaattagag ccagcagtga ccaaagaact agaccaaaat catcaagatc
     2821 atcccagcat caaatttctg caaaattatc aagtagaaac gggacgccct ctttttactc
     2881 catttacctt tcacctcgac ctaactttgt ctgaagaaga actactggct aagatgacta
     2941 gcaaaactcg ctacaacact cggttagcga ttaaaaaagg ggtcaccgtc gtcgaagaca
     3001 atagcgaaca aggtttagaa gattacctca aaattcttga agagaccacc aaaaggcaag
     3061 ctttttatgc tcattcgcct gattactttc gtcaaatgtg ggctactctt aaagatagca
     3121 atattatgca tatcttaaaa gcaagttttg aagggcaagt attggttgtc tggattctct
     3181 ttgtttttaa tgatgtactt tactatccct atggtgcttc gagtagtcaa catcgtgagg
     3241 taatggccag caatttaatg atgtgggaag ctattcgctt tggtcaaaaa caacaatgtc
     3301 aattatttga tatgtgggga gccttagggc ccaatcctga ccccaaagat ccttggtatg
     3361 gttttcatcg ttttaaacgg agttatggtg gtaaattagt cgaatatatc ggtacttatg
     3421 atttggttaa caattatcca atctataaaa tttacaactt agcagacaat tggcgttgga
     3481 aattcttgcg tttcaaaaaa aagtttatta agctataaac aatataaaaa aggaaactgc
     3541 ctatgaaaaa ttttgccatt aatggtctgg gtcggatcgg tcgcctcgct tttcgcgttt
     3601 ggtttgcaaa acaccgccaa gaacttgatc taaaagtcat taatacatct ggatcaatgg
     3661 atcttgaggg ttgggtcaaa ttgctcaaat acgatagcac ctatggaacc ttcagcgaca
     3721 aaatcacttt tgaacaatta aaaaaagttg aggaattaaa agaaggagaa attgaattag
     3781 gttacatcat ggttgccgat cacaaaattt tagtcactgc tgaacgcgac ccagctaaaa
     3841 taccctggga aaaatacgga gtatatagtg ttttagaagc tactggggct tttgataaag
     3901 caaaaaaagc agctcttcat ctaaaaggcg gagccaaaaa agtacttttg tctgcacctg
     3961 gtaaagaaga tccagagggt ttgattagta ctggagtgat tagcgtcaat gagcttgatc
     4021 aaactaaaaa tattttttct aatgcttctt gtaccaccaa ttgtgtggca ccaatcgtca
     4081 aaattattga agataatttt gggattgaaa aagcaatttt aactactatt cattcctata
     4141 ccagtgacca aaatttacat gataattcac ataaaaagga tttgcgtcgc gctagagcag
     4201 cagccattaa tctggtacct acttccacag gcgctgccaa agctactgcc gacatcattc
     4261 ccagcttgaa aaataagttt gatggtttag ctatccgcgt gccaacgcct accgtgtcat
     4321 tgtctgatat tacttttgtt ttaagcaaag caagcaacct ggaagaaatc aaacaagtat
     4381 ttattgaagc tgccaagggc aaatataagg gaattgtcgc tgttagccaa gaacccttag
     4441 tttctagcga ttttattggt tgtgaataca gcgccgtagt tgatttaccc ctgatccaac
     4501 ttattgatgg caatctctta aaagttgttg cttggtatga caatgaatgg ggatactcct
     4561 gtcgcttaat ggagcagtta agtcaaattt aaactaaaca gcttggtgat catagggtat
     4621 aatgctagcc tatggcaaaa agcacatcgg aaacaaaaac caaaaaagcg actgaaacaa
     4681 ataattccaa aagtggaact aaaacagcca aaaaaactaa ttctactcaa gaagcgaagc
     4741 catcgctgat agcgccaaat gataaaatca cgatcaccat tccttggtca gaattaaaag
     4801 gtccttacga caaggcgtta gctgatttgg ctaaaaaaat gaaaatcgag ggctttcgtc
     4861 aaggtaaagt tccgttaaac atcgccgaga aacaaatttc tttggaaaaa gtagctgaaa
     4921 ccatgctaca agatatttta cctaaaaaat atattgaagc tctgcaaaaa gcaaaaaaac
     4981 agcctattac cgcccccgaa tatcacctaa tttcgatcaa aaaaggtgaa gattgggtgc
     5041 tggaagccca ttttgctgag gctccatcaa ttgatctaaa aaattatgaa aaatatatta
     5101 aagctggaaa acaagcagcc aaagaattca tcaaaaagca caatcaacag gcagaacaaa
     5161 aaaatccccc aaaagtgaca aaaaccaata ccagcgataa ggatcagcag caaaagtcag
     5221 aacagataag caaactctcc ccagatcagg aacaagaaat ttattatcag cacatcttgc
     5281 gcgagcttgt tgttaatatt cgacccagta ttgctgaatt attattaagg cgagaaactc
     5341 aagctgaatt tgaaaatttt aaggagcaat tagcgagcta caacatcagc gttgaaaagt
     5401 atttagaaca acgtcaaatt gatttagagc aattaggtaa tgaaatagct ggtacagtct
     5461 taaatcggtt acaaattgat tttattctgg ctgccattgc caaagagcga caactcaaag
     5521 tggatgatca agctttaaaa aaagctctcg ctgaaatcaa ggacgacaaa ctccgtgacc
     5581 aaattgccaa ccatgagcaa tatctaacca gttttaaagc tcaattattg cgtcgtcaaa
     5641 ctattgaaac cttagtcaaa gattaatgtt atagttttga ttaatccaga ggtaaaactt
     5701 gactttgtat cactttgatc ctataatata agctcgaatt aattaagcaa ttttagtatt
     5761 aattgtcctt ttttttaaac acgctacaat catgaataac ttgaatcaaa atattagtgg
     5821 ccaagcggcg agaaagcaat cgactaatca aagtggcgct cttgctcgag cgctgttgga
     5881 gagtgaaaaa aatgctggta cctatacacc ccagaatgag ccaccaaaac ccgaactcga
     5941 tccttttgct caggctttaa gtcgcgctgg tggaagtttt ttgtcacctc taaatgaaca
     6001 atcagccaac gaacaagcag atctgttggc tcagcaaaaa aaagaagccc tgcgacgtca
     6061 actacatgag cgaattaatc ccgtcgacat gcacgaaatt tttgctgccc aagcagaaaa
     6121 gaatttgaaa gagttagaag ctactagaga tgagttaaaa atgctggctg aagaggtcaa
     6181 agagttagat caagaaattg attcagccct gagtagagaa gtcgtcgatc ccggtattac
     6241 tggtattggc ttttttaact ttttccataa acttcgtgaa tggattggat tgctacgtga
     6301 acaagtttct agtgctaaaa cctggatgaa tcaagtttca ggcaagggta aacgcggacg
     6361 cggcaccgct ttcgatttca gcaaaagtaa aaatgtccac gatgccatga atagcgaaag
     6421 aaattttgga atgaatatgg gtggttaaaa acccaaaatg tgagcttgag gggactcgaa
     6481 cctctgacct cttcgatgtc aacgaagcgc tctaaccaac tgagctacaa gctcgatcac
     6541 taaacgtcct ggattatact ggatcacgta ggtcaaatca atctgtttat ggtcggttgt
     6601 ctcatgttat aataatctct ggaaataatt taatcttcgc agaaaaaacc gtttccagag
     6661 attacttact agttttttgt ttaatttata gtataattct aaacctcagg tgatcagaaa
     6721 aaaatacaac aaacaaagaa aagataactt atttgtgaaa gcgaatgctc atattcactt
     6781 tccacaagtg cgcgttttaa cagaacatgg agaaatggtg ggcattatgt catccagaga
     6841 ggcttttcag caagccatgg atgaaggcaa agatttggta ttaattaccg aaaagactca
     6901 gccaccagtt gttaagatta ttgaattatc gaaatttaaa tatcagctga aaaagaaaga
     6961 agctgataat cgaaaaaagt caaaaattca agatcttaaa gaactacgtt tctcaccagt
     7021 aatagaagag ggtgattacc aaactcgact caaacagtta agagagtttt taactaaggg
     7081 tcataaggtc aaaattactc tgttctttag agggcgacag atcgcctata aagaagctgg
     7141 ttatgaagtc tttgctagaa tcattcagga cactgaagat ctagctaaag tggaagttga
     7201 gccacgatta attggtaaaa aactccaagc tcagctcgcg ccactaggga aagaagataa
     7261 gaaataagga aagtcatgaa aaaaatcaaa gttaaaacca gaaaaggcgc cgctcgtcgt
     7321 tttcatatta ctggtagtgg taaggtgatg cgccgaattc aaaatcgacg tcacttaaga
     7381 agaaataaga gtaaaaaagc gattcgtaat tatcgtattc ccgtcgaagt taaaggccct
     7441 tacgctaaaa aaattagaaa gatgctcggt cttaattaaa ttttcagaaa gatattatgc
     7501 caagaactaa aactggtgtt gtcagacgtc gccaccacaa aaaaattcta aaattaaata
     7561 agggttttcg cggtgctaat cgtcgtctat acaagagagc caaagaagct tcactacatg
     7621 ctggacagta tgcttatgtc ggtcgcaaac tgcgcaaaag agacatgcgt aaactgtgga
     7681 ttattcgaat taatgctggg ttaaaacaaa tcaaccaaca attcaattat tccagattca
     7741 tcaataattt aaaaaaagct aaaatcgctc ttaaccgtaa agttttaagt gaattagctg
     7801 ttagtgattt tgacaccttc aaagaaattg tggtcaaaag ccaaaaataa atatctcaac
     7861 acaacaacag tgtgccccga acggcagtgg ttagcaaaat taggtagact tggattgtgc
     7921 gacttcaagt aatttgctca atcctgctga aagctgttaa aatacaagga gccaaataag
     7981 aaagaaaatt aaacaatgtc tcactaccaa gatttagtta agcaaagcca agcattactc
     8041 agtcaatttc aagcagaacg caaacaatta aacgctgagc agttaagcat taaatatctt
     8101 ggtaaaaaag gtttgtttaa tgaattagct ggtcagatta aaaatctaaa aagaagtgaa
     8161 gataaaaaag aatttggcca aatactgaat caattaaaaa aagacttgga aacagccttg
     8221 gcagacagtc aaaaacaaca agaactggtc catgagcgcc tagatctaag tttaccagga
     8281 ataaaatcaa acagcggtca tttacatcca gttagtcaag ccattgaaga aattagtcaa
     8341 atctttgaaa gaattggttt tgtgagaggc gattacccag aggtagattg ggatcattac
     8401 gtttttgctg ctctcaatat gcccgctaat catccagcgc gagatgagtg ggaaactttt
     8461 tttgtcgatc aacccgctga tcctaaactg ggcaaagtcg tgcttaccac tcacacctcc
     8521 aatggtcagg taagagaaat ggaaagagtg aaggggcgat tacccattcg catgatcaat
     8581 a
//
LOCUS       DAOSEW010000022         8463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_7157_length_8463_cov_4.511299, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000022 DAOSEW010000000
VERSION     DAOSEW010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 8463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8463
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7157_length_8463_cov_4.511299"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PLJ02_01350"
     CDS             <1..246
                     /locus_tag="PLJ02_01350"
                     /inference="COORDINATES: protein motif:HMM:NF012265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative dsRNA-binding protein"
                     /protein_id="HOI05094.1"
                     /translation="ILASKAYRDQKSLLQEAVQARSLPTPAYRVIKEEGPDHNKTFTV
                     EVLINQEVWGVGQGNSKQRAQQAAASMALKKIAEGKQ"
     gene            296..757
                     /gene="rpsP"
                     /locus_tag="PLJ02_01355"
     CDS             296..757
                     /gene="rpsP"
                     /locus_tag="PLJ02_01355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOI05095.1"
                     /translation="MLKIKLARFGKRNQPHYRIVVNEARDKRDGKYIALLGNYSPSYQ
                     PKVLELDLKAYENWVAKGAQPTATVAALYQRVKSGKLFPEKKARLSKKAQAKLKAAKE
                     EKVEKKAEKPVKETGDQVAEQAEAKPADQVAEQEAPAKPADQVTEQAEAKN"
     gene            765..1001
                     /locus_tag="PLJ02_01360"
     CDS             765..1001
                     /locus_tag="PLJ02_01360"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOI05096.1"
                     /translation="MKTLLEFVLLRLIKFPEDLKIEEVEEAGYRKYLIQLNEEDYGRV
                     IGKGGKVIRAIRKLAQVRAIKEQFRVRIELVEDK"
     gene            complement(1042..1989)
                     /gene="pilO"
                     /locus_tag="PLJ02_01365"
     CDS             complement(1042..1989)
                     /gene="pilO"
                     /locus_tag="PLJ02_01365"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HOI05097.1"
                     /translation="MPIEKKEEVNFKLIWNTRKEYVLAILCFVLAFLIFFMVILRQIE
                     PVKDVFAELDTTNQELEKFQTKADELDNISVNGEFDKMTEINDVLPSYKPLLELLNNL
                     NSVAVESETIIKNFSLSPGEIATDSTITTRTRRQKYYDELVLEFSVTGSLGNVQNFMT
                     LIEQVTPISTITNIALSQQVNENSAIETTANLKLKTFYFTQPITVTLTEPLPPIDDEQ
                     LILLEEIKKLIPNNLPTQEEVITNDRGNLFGLEGMTVEELETQLQASELEMGDVQWQT
                     VDETGQPIEQNQAPIEQNQAPIIENQAPIEQNQTPIIEN"
     gene            complement(1990..2661)
                     /locus_tag="PLJ02_01370"
     CDS             complement(1990..2661)
                     /locus_tag="PLJ02_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05098.1"
                     /translation="MTEINFLSSRTRQKQKQQEKDKKLFKIASIVLGVVVLILVSIIA
                     LKLVINLRIKDKTETIAELKKTIVSKEEVELSYLIFVNKLNAIEEIYAARSDKQTAMN
                     YFADLMADVATITGMTYDEESGGLILQLDHQNVFFLEKSMNIIDSPLVTDVYKNVKKG
                     SLSREDQGNYLLRLTIQLKTIEDLGLLENNEVDGEVIDTETGAENAINDQMELDMELD
                     TEEFN"
     gene            complement(2658..3668)
                     /gene="pilM"
                     /locus_tag="PLJ02_01375"
     CDS             complement(2658..3668)
                     /gene="pilM"
                     /locus_tag="PLJ02_01375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HOI05099.1"
                     /translation="MSITALDIGTHTIKAISAKNGKQIQVLKAVDIPNSLGLVIPQSD
                     QETTQFSEMIANLLNDYKLDASDVRLSLPESIIANKVIEVPVLTDAELASAVGWQAEQ
                     YIPIPKDDLSLEYQVLFRPDKKMPDAKMKVLLVASRKSFVRKLNDIFLNLGIEPTVME
                     TQMFSVIRALNLRSDDPETLIVHMGASNLDLAVVANGMFDFIFSTKNGAQLLTNAIAQ
                     SFALDLKQAEEYKITYGLNPEQLDGKLIQVILPIVNNVVVEIQKALRYFSQLHPGQTI
                     KRVVLSGGPAEMIGFSQYLAQQINTEVFLMAPFAEAKGQIPETKHLHFTVCMGLVMRK
                     LS"
     gene            complement(3694..4482)
                     /locus_tag="PLJ02_01380"
     CDS             complement(3694..4482)
                     /locus_tag="PLJ02_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05100.1"
                     /translation="MSKIFSRQMSGQIGIIVVLITAAILTFGLSVANRVVQENKMVVD
                     RSDSIRTFNTAETGVDEALNQIYQFETGLDSSLNTGVVFEDDYNQISIATSQSFEGYL
                     NQGESLRVDLNNQAGSITLNWSKTPCSASQKVGLLLTFLHRSGDEYQSHYYLIGHSDC
                     QYSSYQNFIPATYNAASALKYSYTLALPAASNASLYIQTVGTGTDLQIGGGVGLISSA
                     QYEIESLARSEGDTSNKTIEVSKSLPSAPGFMSFALFSGGNILK"
     gene            complement(4547..6709)
                     /locus_tag="PLJ02_01385"
     CDS             complement(4547..6709)
                     /locus_tag="PLJ02_01385"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOI05101.1"
                     /translation="MYQLKNSLDTVHGIGDSWQKALAKKNIHSILDFLLYLPLHYEDR
                     SQIFSIAQLKRVNNSLLIDQAEFDQTQHQNKDYQSKQDYFTLIVQVKDFRQYYKGRLL
                     ISRAMLVDKTGELPAIWFNNKFLKSQLQPDQYYFASGQLKNNTLVQAKLEALTTDSPH
                     TARLVPVYSQISPFKQGSLRRLQKEIIDHLNPQEKIVEGKPIEDQQIKVEPAKSKPTT
                     SNLQLFQNAKDFFQALHFPENTQEIIQAREQLALEEIIFLINKAKKLKEQWQSSHLAP
                     SIKVDKKQLIPTDLPFSLTKAQKRAVKEITSDLGKNVAMNRLLIGDVGSGKTVVAAIA
                     AWHSCLAGHNAVLVAPTQILAKQHFQNIQKLLPKQKIYLVTAQTSQDFQKDTASIYVG
                     THAVINKLEEIKPALIIYDEQHRFGAKQRQNPGAHLLTMTATPIPRSLMLTIFAHLQT
                     SVLDEMPANRQMAKTWLVPPSKEKAAIIWLAEQLLQSPSKQAFIVCPFIDPSQHSALE
                     NIAAVKDSLTAVHKILDEYYQEKKIAKSKQIKTGILHSRLQKEEQKRVIEQLYNAKLQ
                     ILVSTPMIELGVDLPNADFMIIQAAERFGLASLHQLRGRVGRVGQDSFCLLFTTHPNQ
                     EKNRETNLVAKSQARLKIFCREKDGLKLAQLDLKQRGAGDIFGSAQSGFNKLQFASWT
                     NLNIIKEAKRISDQRENYQSVLTPYFEKQELITEINNN"
     gene            complement(6835..8049)
                     /gene="tyrS"
                     /locus_tag="PLJ02_01390"
     CDS             complement(6835..8049)
                     /gene="tyrS"
                     /locus_tag="PLJ02_01390"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOI05102.1"
                     /translation="MSNKLNTNPEAIHKVLSRGVEHILPNADSLAKLMSEKAITLYLG
                     IDPTGAELHLGHSVPLRKLQDFAELGHKVILLVGNGTVKIGDPTGRDQSRPMLSDAEI
                     NANFANWQRQASKILDFSMIDIRYNADWLDQLTYSDLLKLMAKTTVQQLLERDMFQDR
                     LRKNLPIHAHEIIYPLLQGYDSVVMDVDLEIGGNDQTFNMMMGRQLQNLYNQKEKWVL
                     TTPLIEGTDGRKMSKSYKNYIALEDEAVEVYGKIMSISDELIIKYFEVLTRVDMAKIE
                     EMKKALDQGENPMQFKKQLAWQITSDLHGEPSAKKASEYFAQSIQNDAIPDRELFTDL
                     IVKVEAKAGQPRSSNNIFSNSQLARLFEQGAVKLLPEQKLINNWQEFLSLPDGSQLKI
                     GKHRFYQLKIKK"
     gene            8097..>8463
                     /locus_tag="PLJ02_01395"
     CDS             8097..>8463
                     /locus_tag="PLJ02_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05103.1"
                     /translation="MDDLSEQTVITDELKPPAPKRKISLWRRLQWLLRRLRTKLVIGW
                     EKFLYLIMPKKSRLHRNKLSPSEKKLRRIKTWRTIALVFCGLVVLGIIVFLVMFFWFS
                     KDLPQPGEVIRREGYSSKIY"
BASE COUNT         2464 a         1545 c         1671 g         2783 t
ORIGIN      
        1 attttagcta gcaaagctta tcgtgatcaa aaaagtttat tgcaagaagc agttcaagct
       61 cgctcactac cgacaccagc ctatcgcgtc attaaagagg aaggcccaga tcacaacaag
      121 acctttaccg tagaagtctt gattaatcaa gaagtttggg gagttggtca aggtaacagt
      181 aaacaacgag cgcaacaagc agccgccagc atggctctga aaaagattgc tgaaggcaag
      241 caatgatgaa actcgccctt gagatattcg tctttttcta gtagaatcct ccggtatgtt
      301 gaaaattaaa ttagctcgct ttggcaaaag aaatcaacct cattatcgaa ttgtagtaaa
      361 tgaagctcgt gataaacgtg atggtaagta catagcccta ttgggtaact attcccctag
      421 ttatcaacct aaagtgcttg aacttgatct caaagcctat gagaattggg tggctaaagg
      481 ggctcagccc accgccaccg tagccgctct ttatcaaaga gttaaatctg gtaaattatt
      541 tcctgagaaa aaagctaggt tatctaaaaa agctcaggcc aaattaaaag ctgctaaaga
      601 ggaaaaggtt gagaaaaaag ccgaaaaacc agttaaagaa actggcgacc aagtagcaga
      661 gcaagctgaa gctaaaccag ccgaccaagt agctgagcaa gaagccccag caaaaccagc
      721 cgaccaagta accgagcaag ctgaagctaa aaactaaaac taaaatgaaa actttattgg
      781 aatttgtttt actgcgatta attaaatttc ccgaagattt aaaaattgag gaagtagaag
      841 aagctggcta tcgcaagtat ttaattcagc tcaacgaaga agattatggt cgggtaattg
      901 gtaagggtgg caaagtgatt cgagctatcc gcaaattagc tcaagtgaga gctatcaagg
      961 aacaattccg cgtacggatt gaactggttg aagataaata atggttgctg attacgcaat
     1021 ggtgttgatt gattgtctct gttagttttc aataataggc gtttgatttt gctcgatagg
     1081 tgcttgattt tcaatgatag gtgcttgatt ttgttcgatg ggtgcttgat tttgctcaat
     1141 gggctgacca gtttcatcaa cagtttgcca ctgtacatct cccatttcca attcactagc
     1201 ttgcaattgg gtctctaatt cttcgacggt catgccttct aaaccaaaga gattgcctcg
     1261 atcattggtg atgacttctt cttgagttgg taaattgttg gggattagtt ttttaatttc
     1321 ttccagaagg attaattgtt catcgtcaat tggtggtagg ggttcagtta gagtgacggt
     1381 gattggctga gtaaaataaa aagtttttaa ttttaaatta gcggtagttt ctatcgcact
     1441 attttcattg acttgttgac tgagggcaat attggtaatg gtgctaatcg gagtaacttg
     1501 ttcgatcagg gtcataaaat tttggacgtt gcctagagat cctgtgacag aaaactctag
     1561 aaccaactca tcataatatt tttgtcttct agttctggtt gtaatagtgc tatcagtggc
     1621 aatttcccct ggagatagtg aaaaattttt gattattgtt tcactttcaa ctgcgacact
     1681 attcagattg ttcagaagtt ctaaaagtgg tttgtaggat ggaagtacgt cattaatttc
     1741 ggtcattttg tcaaactctc catttacaga aatattgtct aattcatcag ctttggtttg
     1801 gaatttctct agttcttgat tagtagtatc gagttcagca aaaacatctt tgactggttc
     1861 gatttggcgt aaaatcacca tgaaaaaaat taaaaaagct aaaacaaaac ataatatagc
     1921 caagacatac tctttacgag tgttccaaat caacttgaaa ttgacttctt cttttttttc
     1981 tattggcatt taattaaatt cctctgtatc aagttccata tcaagttcca tttgatcatt
     2041 gatggcattc tctgcaccgg tttctgtatc aattacctca ccatcaacct cattgttttc
     2101 caacaagccg agatcttcaa ttgttttaag ttgaattgtc agtctaagca ggtaattgcc
     2161 ctgatcttct cttgatagtg aacccttttt cacattttta taaacgtcgg tgactaaagg
     2221 agaatcaatg atgttcatac ttttttctaa aaagaacaca ttttggtgat cgagttgcaa
     2281 aattaaacca ccactttctt catcataagt cataccggtg atggtagcga catcggccat
     2341 cagatcggca aaataattca tggcagtttg cttgtcactg cgggctgcat aaatttcttc
     2401 aatagcattt aatttgttga caaagattaa ataggataac tcaacctctt ctttactaac
     2461 gatcgttttt tttagttcag ctatggtttc tgtcttgtct ttaatgcgca gattaatcac
     2521 cagttttaag gcgataattg agacgagtat taaaactact actccgagca caatgctggc
     2581 tattttaaag agttttttat ccttttcttg ttgtttttgt ttctgtctag ttctgctaga
     2641 caaaaaatta atttcggtca tgataatttt ctcatcacta accccataca gacagtaaag
     2701 tgcaaatgtt tagtttcagg aatttggcct ttagcttcgg caaatggcgc cattaaaaat
     2761 acttcagtat taatttgttg tgctaaatat tgactaaaac caatcatttc agctggtccg
     2821 cctgataaaa ctactctctt aatggtttgt cctgggtgta attggctaaa gtagcgcaac
     2881 gctttttgaa tttccacgac cacattattg acaataggta aaattacttg tatcaactta
     2941 ccatccaatt gttcaggatt taaaccgtag gtgattttat attcttcagc ttgttttaga
     3001 tctaaagcaa aactttgggc aatagcattg gttaaaagtt gcgccccatt tttggtgcta
     3061 aaaataaaat caaacatgcc attagcaacg acggctaaat caagattact tgctcccatg
     3121 tggacaatca gggtttctgg gtcatcgcta cgtagattta aggctctaat gaccgaaaac
     3181 atttgcgttt ccatgaccgt gggttcgata cctaaattaa gaaaaatatc gtttaacttt
     3241 cgaacaaaag attttcgaga agcaactaac agtaccttca ttttggcatc aggcattttt
     3301 ttgtctggac ggaataagac ttgatattca agggataagt cgtctttggg aatgggaata
     3361 tactgttcag cttgccagcc aacagcactg gctagttcgg cgtcggttaa aacagggact
     3421 tcgatcactt tgttggcaat gattgattcg ggtaagctca gacggacatc actggcatca
     3481 agtttgtaat cattaagcaa attagcaatc atttcactaa actgagtggt ttcttggtcg
     3541 ctttggggaa tgactaaacc cagactattg ggaatatcaa ccgctttaag cacttggatt
     3601 tgtttaccgt ttttggcgga gatagctttg atggtatgag taccgatgtc tagggcagta
     3661 attgacataa cttacattta ttttatgtat tttctatttt aggatattac ctccactaaa
     3721 taaagcaaaa ctcatgaagc cgggggcgct aggcaagctt ttactgactt caatagtttt
     3781 attactagta tcaccttcgc ttctggctaa agattcaatt tcatattgag cactactgat
     3841 taaaccaaca ccaccgccaa tttgtaaatc ggtgccagta ccaacagttt ggatatataa
     3901 agaagcattg ctagcagcgg gtaaggctaa agtataacta tattttaagg cactggctgc
     3961 attataagta gcagggatga aattttgata gctactatat tggcaatcac tatgaccaat
     4021 caggtagtaa tggctttggt attcatcacc acttcggtgc aaaaaggtta atagcaaacc
     4081 gactttttga ctagcagaac aaggtgtttt tgaccaatta agggtaatac tacctgcctg
     4141 gttgtttaag tcgactcgca gactctctcc ttgattgaga taaccctcaa aactttgact
     4201 agtagcaatt gatatctgat tataatcatc ttcaaaaact acacccgtat ttaaggaaga
     4261 atcaaggcca gtttcaaatt gatagatttg attaagggct tcgtcaacac cagtttcggc
     4321 agtattgaaa gtcctaattg aatcactgcg atccaccacc attttatttt cttgaaccac
     4381 acgatttgct actgacaggc caaaagttaa aatggcagca gtaattaaga cgacgattat
     4441 gccaatttga ccactcatct ggcgagaaaa aatttttgac atatactcca tcataaagca
     4501 aaaaaggcgc aagaacaagc gattttttag cgcatttttt gctcaattaa ttattgttga
     4561 tttctgtgat taattcttgt ttttcaaaat acggagttaa gactgattga taattttctc
     4621 tctgatcact aattcttttg gcttctttaa taatgttaag attggtccaa gaggcaaatt
     4681 gcaatttgtt aaagccactt tgagcgctgc caaaaatatc tcccgcccct ctttgcttca
     4741 aatctagttg agctaatttc aagccgtctt tttctctaca gaatattttt aatcttgctt
     4801 gactttttgc aaccagatta gtctcgcggt ttttttcttg gttaggatgg gtggtaaata
     4861 ataaacaaaa gctgtcctga ccgacgcggc ctactctgcc ccgtaattga tgcaagctag
     4921 ctaaaccaaa acgctcagca gcttgaataa tcataaaatc agcattgggt aaatcgacgc
     4981 ccagctcgat catcggtgtg ctcactaaaa tttgcagctt tgcattataa agttgctcaa
     5041 ttacccgttt ttgttcttct ttttggagtc gggaatgtaa aatcccagtt ttaatttgtt
     5101 ttgatttagc aatctttttt tcttgataat actcgtctaa tatcttgtga actgctgtta
     5161 aactatcttt aaccgctgct atattttcca aagcgctgtg ctgagaagga tcaataaacg
     5221 gacagacaat gaaagcttgt ttgctcggcg attgtaataa ttgttccgcc aaccatatta
     5281 ttgccgcttt ttcttttgat gggggtacta gccaggtttt agccatttgt cggttagcag
     5341 gcatttcgtc taaaacgctg gtttgcaggt gggcaaaaat agtcaacatt aaactgcggg
     5401 gtattggtgt ggcagtcatc gttaaaaggt gcgctcccgg attttgccgt tgtttagcgc
     5461 caaaacggtg ttgttcatca taaataatta aagcaggctt aatctcttca agtttattga
     5521 tcacagcgtg ggtgccaaca taaatactgg cagtatcttt ttgaaaatct tgactggttt
     5581 gagctgtaac tagatagatt ttttgtttgg gtaatagttt ttggatattt tggaaatgct
     5641 gcttagccaa gatttgcgtt ggagctacca aaacagcatt gtggcctgcc agacagctat
     5701 gccaggcagc aatagcggcc acaactgttt taccgctacc aacatcacca attaataagc
     5761 gattcatcgc tacatttttt cccaaatcgc tcgtgatttc tttgacggct cgcttttgag
     5821 ctttggttaa gctaaatggt agatcagttg gaatgagctg ttttttgtcg actttaatgc
     5881 tgggcgctaa atggcttgat tgccactgct ctttaagttt tttcgcttta ttgattagaa
     5941 aaataatttc ttccagggct aattgttctc tagcttggat aatttcttga gtattttcag
     6001 gaaaatgtag ggcttgaaaa aaatctttgg cattttgaaa aagttgaagg ttactggttg
     6061 ttggtttgct ttttgctggc tcgactttta tttgttggtc ttctattggc ttaccttcaa
     6121 caattttttc ttgaggattg agatgatcaa taatctcttt ttgcaatctt ctcaaacttc
     6181 cctgcttaaa gggactaatt tgactataaa ctggcactaa gcgcgcggtg tgaggactat
     6241 cggttgtcag tgcttctagt ttggcttgga ctaaagtatt gtttttcaat tgaccagacg
     6301 cgaaataata ctgatctggc tgcagctgac tttttaaaaa tttgttatta aaccaaattg
     6361 ctggtaattc accagtttta tcgaccaaca tggcccgact gattagcaat cgccccttat
     6421 aatattggcg aaaatcttta acttgcacga taagggtaaa gtaatcttgc ttggattggt
     6481 aatctttgtt ttggtgctgg gtttgatcaa attcagcttg atctataagc aaagaattat
     6541 tgacccgttt taattgagca atcgaaaaaa tttgtgagcg atcttcgtag tgaaggggta
     6601 aataaagcaa aaagtctaaa atactgtgga tatttttttt ggctaaagcc ttttgccaac
     6661 tatcaccaat accatgaacg gtgtcaagag aattttttag ttggtacata tttttctaat
     6721 ttagaaaaaa gcaaaaagta agctagcaat gctactaaaa accatgatga ctacggcgaa
     6781 aatgacaaag aaacgaataa tcttttctct ttttttgcga ctcaatgaca tagcttactt
     6841 tttgattttt aattgataaa aacgatgttt gccaattttg agttggctac catctggtaa
     6901 agataaaaac tcttgccaat tgttgatgag tttttgctca ggtaataatt taaccgctcc
     6961 ctgctcaaat aagcgagcca attgactatt gctaaaaatg ttattgcttg atcgtggttg
     7021 acctgctttt gcttcaactt ttacaattaa atcagtaaat agttctcgat cgggaatggc
     7081 atcattttga atactttgtg caaaatattc actggccttt ttcgctgatg gttcaccatg
     7141 taaatcactg gtaatttgcc atgccaattg ttttttaaat tgcatgggat tctcaccctg
     7201 atctaaagct tttttcatct cttcaatttt agccatatca actctggtta aaacctcaaa
     7261 gtatttaata attagctcat cgctgatgct catgattttg ccgtaaacct caaccgcttc
     7321 gtcttctaga gcgatatagt ttttatatga tttactcatt ttacggccat cagtgccctc
     7381 aataagtggc gtagttagca cccatttttc tttttgattg tagagatttt gtaactgccg
     7441 acccatcatc atattaaagg tttgatcatt cccaccaatt tccaaatcga cgtccatcac
     7501 gaccgaatcg tatccttgca gcaagggata aataatttcg tgggcgtgaa tcggtaaatt
     7561 tttacgaagt cggtcttgga acatatctct ttctaagagt tgttgaacag tagttttggc
     7621 catcaatttc agtaggtcgc tgtaagtaag ttggtctaac cagtcagcgt tataacggat
     7681 atcaatcatg gaaaaatcaa gaattttact ggcctgtcgt tgccaattag cgaagttagc
     7741 attgatttct gcatcactaa gcatcggtcg actttgatcc cgaccagtag gatcaccaat
     7801 tttgaccgtg ccgttgccaa ctaagagaat aactttatgg ccgagttcgg caaagtcttg
     7861 taatttacgc aaaggcacgc tgtgaccaag atgtagttcg gcgccagttg gatctatccc
     7921 caagtaaaga gtaatggctt tttcactcat taatttggct aacgaatcgg cgttgggtag
     7981 aatatgctct actccacggc taagaacttt atgaatggct tcggggttgg tatttagttt
     8041 atttgacata gaattgtttt attgtagcat gaggtcaagt ttggtagaat gatgacatgg
     8101 atgatttatc tgagcagacg gtgattactg atgaactgaa gccaccagct cccaaaagaa
     8161 aaatttcttt atggagacgc ttacaatggt tgctacgacg cctgagaacc aagctagtta
     8221 ttggttggga aaagttttta tatttaatta tgccaaaaaa aagtcggtta catcgcaata
     8281 aattaagtcc aagtgagaaa aaactcagac gaattaaaac ttggcggaca atcgctctgg
     8341 ttttttgtgg tttggtggtt ttgggtatta ttgtgttttt ggtaatgttt ttttggtttt
     8401 ctaaagatct accacagccg ggtgaagtaa ttcgacggga aggttatagt agcaagattt
     8461 atg
//
LOCUS       DAOSEW010000023         8296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_7363_length_8296_cov_4.147798, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000023 DAOSEW010000000
VERSION     DAOSEW010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 8296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8296
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7363_length_8296_cov_4.147798"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..314
                     /locus_tag="PLJ02_01400"
     tRNA            238..314
                     /locus_tag="PLJ02_01400"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Val,seq:cac)
     gene            333..767
                     /locus_tag="PLJ02_01405"
     CDS             333..767
                     /locus_tag="PLJ02_01405"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOI05104.1"
                     /translation="MNLQEQLMEDMKTAMRARDAERLGVIRLIRSEVKNFEIDHGVAD
                     DAAVVKIIKKMLNQQLDALEDFKKAAREDLINETESIVKILQSYLPEQMSDQELEKFI
                     QDFVEQSAVKDFGPLMGKLTQALGSKADGSRIASTLKKLLNK"
     gene            814..1470
                     /locus_tag="PLJ02_01410"
     CDS             814..1470
                     /locus_tag="PLJ02_01410"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOI05105.1"
                     /translation="MNQTVKTQTVGDLLSKHRKKAGLELSDIAQQTFIKIEYLQAIEN
                     NNFADLPNASYAKAYISNYAKLLGLNPRPLLAILRRDYQESAKGRLLPREFLKDSFKK
                     SNFFSPIRLTFSLIVVIFVSFLIYTGWQWYALSRPPRLEVFQPENNQTTAAKVLVEGQ
                     TVMDAIVLVNAQPVALQAGGFFSTELYFDIEGPTTITVRATDQRGKTSTVQRSIQVKF
                     "
     gene            1542..1877
                     /locus_tag="PLJ02_01415"
     CDS             1542..1877
                     /locus_tag="PLJ02_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05106.1"
                     /translation="MSDILPLVLTISVASLTVVLVVLAVKLILVLGQVKQSLEKFNLA
                     IDKANQVIDTTQEKIEGILNPFHSLGAFITNFTAGLKITEGFMNWLNRDKGHGTEVAK
                     DKSAGQKKK"
     gene            1881..2237
                     /locus_tag="PLJ02_01420"
     CDS             1881..2237
                     /locus_tag="PLJ02_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05107.1"
                     /translation="MDENKQQGSNFLFGLTIGFSLGALISYFSSENGKKTWEKLANEW
                     EKARVDLYEQGLIESPDVTLDEVKDKYLWHLKNSLLDFKDNLSLALVKLEQSKEKENI
                     HKRIRRQRKKNQFKGI"
     gene            2259..4166
                     /locus_tag="PLJ02_01425"
     CDS             2259..4166
                     /locus_tag="PLJ02_01425"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOI05108.1"
                     /translation="MSSNTLTLSSLRRLYLDYFAKRGHAIVPSANLVPENDPSTLFTS
                     AGMQAMIPYLLGEKHPQGQRIANSQKCFRSEDIDEVGDNRHTTFFEMLGNWSFGDYFK
                     QEQITFFFEFLVDVLQLDPSRLYVSVFAGNKALNIPKDEESALIWQDLFQKRGIEAKI
                     VDQPQAGLQGGRIFFYGEEKNWWSRSGVPATMPLGEPGGPDSEVFYDFAPDQAQQFHQ
                     QSKFAQEPCHINCDCGRFLEVGNSVFMQYQRTATGFRELAQKNIDFGGGLERLLTVLE
                     GQVDVFQSSAFSGIIKKLEEISQKNYQDPDLRAAFRIIADHLKASVMLIADGVLPSNK
                     EQGYLLRRLIRRSLRQAKYLGLEQEFLPDLVPAIVDLYQDFYPQVESKTEHINEVLKN
                     EEQKFNKTLKRGLKEFNRLISNKDQLTADLVFTLYESYGFPFELSIEEAKTRNISIEP
                     AIDSKIAEANQQHAALSRQTAKDRFKGGLADHEAKTTAFHTATHLLQAALRKVLGDQV
                     QQKGSNINSERLRFDFSYTRALNKEELVTVEQLVNQWLQEDLPVSHEILSKEEALKSG
                     AIAFFIERYPDQVSVYTIGDPDHWISKELCGGPHVASTGQIARFGDLEIFKEKAVSQG
                     VRRIYMRFRAE"
     gene            4253..6505
                     /locus_tag="PLJ02_01430"
     CDS             4253..6505
                     /locus_tag="PLJ02_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05109.1"
                     /translation="MPNLLKKIIKGKKEQETEMPVGELMETELFITIILSDQELQAAL
                     VSKNQEIKEYSAIKTYFDRQDLLTQLDQALQDLGPLADEVADCVFVFDETWLEKGDLQ
                     DSKKVIVKDLTDNLSLLPLGQMSISEGIHQARIMKDPHDSSIVLYLRDKDFDLLLIKH
                     GKLLANLKIGRSARLSDDLQEGLARISQELGEQGKYFPNKVFLSSLNLPQKVLNKEQA
                     ELQAFDWTTLSGFLQMPEFVVLSNDFLIKSLSLAASYMLSKNFASKLTQEFSEMTSTD
                     NKDISDQDVASNGPELVSAEKKISSFGIDLSSEKIDLEQALHQQTPLAKSQAEEITND
                     NLQAVDLSSVNSQDQKKIKSKSSSVLKRFFKKNKKSLLIGVGGGLLALITLFFVYNLF
                     LSDVTVKVTANERILQKNLEIILDPQLAESDFTKNLLKVSLEEKEIKGEDTLSTTGIS
                     LVGDKAQGKVKLFNKTFTDKTFSANTIFKYEDIAFVLNEEVTVPAAVSEEEDDGQNID
                     YGSVEVALTAQDIGAEGNIGKDSKLAVADFSENTYSATAIEDFSGGSSREIRVVSQKD
                     LDNLQNQLKENLYAIAVKEFKEESKDGVYYVPTGESRIVDFAYDHEEGDEVETLSLTM
                     TLAVEAVKYSSSDLKQLAEEVLLLDLPENYEFVDEEPSLLSDEPQLMTDGSNRLVLDA
                     ELSAKAKAKINQEALNDLVLGKSLEEAKNNLEEQETIDRAEIILQPAFMGRVLQKLPK
                     DSSRFNFIIN"
     gene            6507..7466
                     /locus_tag="PLJ02_01435"
     CDS             6507..7466
                     /locus_tag="PLJ02_01435"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HOI05110.1"
                     /translation="MAKTNPQLTKQKKQLLKLYRQALRDGVPIQDLNKKVNYFLKQDK
                     ASQQGEKNLDEQIQLNFWGRLPKIVRVGATFLPLALIVFGLALALSALWPIIDYYLPW
                     KKEEEITLLSPIPNSQLLLGNPLISAQAQTEIVENSTTDYSVETEPVILDQDLDFTDL
                     TNWFSDDLENVELSTSEFIEYTLDIPRLGISNAKVRVGGSDLNQSLIHYPGTGMPGQL
                     GAPVIFGHSVLRQFYNPSENNPRRYNSIFSKIMTLEKGDKIYLTYDNVKYTYVVQGKT
                     EVKADDTFILAQRVDVRQLKLVTCVPEGTTLRRGVITAQLVKE"
     gene            7476..7877
                     /gene="ruvX"
                     /locus_tag="PLJ02_01440"
     CDS             7476..7877
                     /gene="ruvX"
                     /locus_tag="PLJ02_01440"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOI05111.1"
                     /translation="MSTILAIDYGLARIGLAISYVGLAEPLTIINNDVTSNEPIIGSQ
                     ALAEIKQIVKAEKVDKIVLGISEQAMAKKILEFFEILQSTFNLDVILVDEVLSSYEVE
                     QRLKELPKKKRRQAIDHYAAAIILENYLDLC"
     gene            7891..>8296
                     /locus_tag="PLJ02_01445"
     CDS             7891..>8296
                     /locus_tag="PLJ02_01445"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOI05112.1"
                     /translation="MKNSPKKPRSFFYFLLIFVIIVISGWAIFISLLQAPNSSDQSTV
                     TFIIPKGEATQNIAQRLKAEGLIRSPLAFRLALYQQGKQTSIQAGSFKLSPSMSVSEL
                     TQVLTKGTDDLWITIPEGWRREEIAASLVKQDL"
BASE COUNT         2726 a         1320 c         1710 g         2540 t
ORIGIN      
        1 aaaaaattct ttgtaatcct cttgccaaaa aaaatcaatc aattgatagc aagactcaca
       61 caacattggt cctaattgtt gacagtgttg acaaaaatca ggaaagatca aagcttggca
      121 aatttgagca atagaaaatt ttttcactga ctaatagttt aatttggctg actgacaaga
      181 agatgacttg cctttctaaa agctaaggct ttgttttata atagagactt aatcgacgga
      241 caattagctc agttggttag agcgcacgat tcacattcgt gaggtcactg gttcgagtcc
      301 agtattgtcc accactaata aaaaggagaa atatgaactt acaagaacaa ttaatggaag
      361 atatgaaaac agctatgagg gctagagatg ctgagcgtct tggtgtgatt cgtttaattc
      421 gcagtgaagt gaaaaatttt gaaatagatc atggggtggc cgatgatgca gcggtggtaa
      481 aaattatcaa gaaaatgttg aatcagcaat tagatgctct agaagatttt aagaaggctg
      541 ctcgtgaaga tttaattaac gaaacagaaa gtatagtgaa gattttacaa tcatatttgc
      601 cagaacaaat gagcgaccaa gagctagaaa aattcattca agattttgtt gaacaaagtg
      661 cggttaaaga ttttggtcca ttaatgggta agcttaccca ggcgctgggt tctaaagctg
      721 atggatccag aattgcgagc actctcaaaa aattgttgaa caaataaggt ttagagcttg
      781 ctttaactag taattctttt tagaattgcc ctaatgaatc aaacggtgaa aacccaaaca
      841 gtaggtgatt tgttaagtaa acatcgaaaa aaagctggct tagaattatc tgatatcgct
      901 caacaaactt tcattaaaat tgaatattta caggcgattg aaaataataa ttttgctgat
      961 ttacctaatg ctagttacgc taaagcttac attagcaatt atgccaaact acttggtctg
     1021 aatccacgtc cacttttggc aattttgcgt cgtgattatc aggaaagtgc caaaggcaga
     1081 ttacttcctc gtgaattttt gaaggattca tttaaaaaat ctaatttttt ttcaccaatt
     1141 cgtttaactt tttccttgat tgtggtgatt tttgtcagct ttctgattta taccggttgg
     1201 caatggtatg ctcttagtcg accaccacgc ttagaagttt ttcagccaga aaataatcaa
     1261 acgacagcag ctaaggtgct agttgagggg caaacagtga tggatgctat cgtgctagtg
     1321 aatgctcaac ctgtggcttt gcaggcaggt ggttttttta gcacagaatt gtactttgat
     1381 attgaaggac caaccacgat tacggtcagg gccaccgatc agcgaggtaa aaccagtacg
     1441 gtgcaaagaa gtattcaggt caaattttaa cttttgttta gtctggctgg taaattcttg
     1501 tagtctatcg tagaatgagc ttataaaaag gagaatgaat tatgtcagat attttacctt
     1561 tagttcttac catttctgtg gctagtttga ccgtggtttt agtagttttg gccgtcaaat
     1621 taattttggt tttggggcaa gtcaaacaat ctttggaaaa atttaattta gcgatcgata
     1681 aggccaatca agttattgat actacccagg aaaaaattga gggtattctt aatccatttc
     1741 acagccttgg cgcttttatt accaatttta cagcgggtct aaagattacc gagggtttta
     1801 tgaattggtt aaatcgggat aaaggtcatg gcactgaagt tgccaaagac aaatcagctg
     1861 gtcaaaagaa aaaatagttt atggatgaaa ataagcaaca aggtagcaat tttttatttg
     1921 gtttgaccat tggcttttcc ctaggagctt taatcagtta cttttctagc gaaaatggca
     1981 agaaaacttg ggagaaatta gctaatgagt gggaaaaagc tcgggtagat ttatatgagc
     2041 agggtttaat cgagagtcca gacgttactt tggatgaagt taaagataaa tatctttggc
     2101 atttaaaaaa ttctctatta gactttaaag acaatctcag tttagccttg gttaaattag
     2161 agcaatcaaa agagaaagaa aacattcata agcgtattcg gcgtcaacga aagaagaatc
     2221 aattcaaagg tatttagtgt aactgttata ataatttcat gtctagtaat actctaactt
     2281 tatctagtct ccgtcgttta tatttagatt attttgctaa gagagggcat gcaatagtgc
     2341 cttcggcaaa tttagtacca gaaaatgatc ctagtacttt gtttacaagt gctggaatgc
     2401 aagcgatgat tccctattta cttggggaga agcatccaca gggtcaaaga attgccaatt
     2461 cgcaaaaatg cttccgcagt gaagatattg atgaggttgg agacaatcgt cataccactt
     2521 tttttgaaat gttgggcaat tggtcttttg gtgattattt taagcaagag caaattacct
     2581 tcttttttga atttttagtt gatgttttgc aactggaccc ctctcgactt tatgtatcgg
     2641 tttttgctgg caacaaggct ttaaatattc ccaaagacga agaaagtgcg ctcatttggc
     2701 aagatttgtt ccaaaaaaga ggcattgaag caaaaattgt tgatcagcca caagctggct
     2761 tacaaggagg tagaattttc ttttatggtg aagaaaaaaa ttggtggtca agatctgggg
     2821 tgccagcgac gatgccactt ggtgaacctg gtggtcctga tagtgaggtt ttttatgatt
     2881 ttgcgccaga ccaagcccag cagtttcatc aacaatctaa gtttgctcaa gaaccatgtc
     2941 acattaattg tgattgcggt cgctttttgg aagttggtaa cagcgtattt atgcaatacc
     3001 aacggacagc aactggcttt cgtgaattag cccaaaaaaa tattgatttc ggtggaggtt
     3061 tggagcgttt attaactgta ctagaaggtc aagttgatgt ttttcaaagc agtgctttta
     3121 gtggaattat taaaaaatta gaggaaatta gtcaaaaaaa ctatcaagat ccagatttac
     3181 gagctgcatt tcgtatcatt gctgatcatc ttaaggctag tgtcatgcta attgctgatg
     3241 gtgttttgcc aagtaataag gaacaaggtt atcttttgcg gcgtttaatt cgccgatcgc
     3301 ttagacaggc caaatattta ggtttagaac aagaattttt gccagatctg gtcccagcaa
     3361 tagttgatct atatcaagat ttttatccac aagtggaatc caaaactgaa catattaatg
     3421 aagttttaaa aaatgaagaa caaaagttta acaaaacgct caaacgtggt ctgaaagagt
     3481 ttaatcgttt aattagtaat aaagatcaat tgactgccga tttggttttt acactttatg
     3541 aaagctatgg tttcccgttt gaattgtcca tcgaagaagc taaaacaaga aatattagca
     3601 ttgagccagc tattgacagt aagattgctg aagccaatca acagcacgct gctttatcta
     3661 gacaaactgc taaggacaga tttaaaggtg gtttggctga tcatgaggcc aagacaacag
     3721 cttttcacac ggccactcat ctactgcaag cggctttgag aaaggtgttg ggcgatcaag
     3781 ttcagcaaaa aggttctaac attaatagtg aacgtttacg ctttgatttt agttatactc
     3841 gagcattgaa taaagaggag ttggtaacag ttgaacaatt agttaatcag tggttgcaag
     3901 aagatctgcc ggtatcgcat gaaattctct ctaaggaaga ggctctcaag tctggagcga
     3961 ttgctttttt tattgaacgt tatcccgatc aagttagtgt ttataccatt ggcgatcctg
     4021 atcactggat atctaaagaa ctttgtggcg gtcctcacgt cgcaagtact ggtcaaattg
     4081 ctcgctttgg cgatttggaa atttttaagg aaaaagccgt ctcgcaaggt gtacgcagaa
     4141 tttatatgcg tttccgagct gaataaatac tcagttcttc aaaattatcg aaaattctgc
     4201 tttaatagag ctgcaggttt tatttaaacc aattctttat tatccaaata atatgcccaa
     4261 tttactaaaa aagattatca aaggcaaaaa agagcaagag accgaaatgc cggtaggaga
     4321 gctgatggaa actgagttat ttattaccat cattctcagc gatcaggaac tgcaagctgc
     4381 tttggttagc aaaaatcaag aaattaaaga atattccgca attaaaactt attttgatcg
     4441 ccaagattta ttaacacaac ttgatcaagc tttacaagat ttaggtcctt tggcggatga
     4501 agtagctgac tgtgtttttg tctttgatga aacttggctg gagaagggtg atttacagga
     4561 tagcaaaaaa gtgatagtta aagatttaac agataattta agtttgttgc ctctgggaca
     4621 aatgagtatt tcagaaggta tccatcaagc tagaattatg aaagatcctc atgattcttc
     4681 gattgttctt tatttacgtg acaaagattt tgatttactg ttaatcaaac acggaaaatt
     4741 actggcgaat ttgaaaattg gccgctccgc tagacttagt gacgatttac aggaaggttt
     4801 agccagaatt agtcaagagt taggcgagca aggcaagtat tttcctaaca aggtttttct
     4861 ttcctcatta aatttgcccc aaaaagtgtt aaacaaagag caggcagaac tgcaggcttt
     4921 tgactggact acattgtcag gttttttaca aatgccagaa tttgttgttc taagcaatga
     4981 ttttttaatt aaatccttaa gtttggctgc tagctacatg ttgtccaaaa attttgcctc
     5041 aaaattaact caagaattta gtgaaatgac cagtacagat aataaagata tctctgatca
     5101 ggatgtggct agtaatgggc ccgaactagt aagtgcagaa aaaaagatta gcagttttgg
     5161 tattgattta agttcagaga aaattgattt agagcaagct cttcaccagc aaactccatt
     5221 ggccaaaagt caagctgagg aaataactaa tgacaatctt caagctgttg atttatcttc
     5281 tgtgaacagt caagatcaga aaaaaataaa aagtaaaagc agtagcgttt tgaaacgttt
     5341 tttcaaaaaa aataaaaaat ctttactgat cggtgtggga ggaggcttgt tggctttgat
     5401 cacactattt ttcgtctata atttgttttt gtctgatgtt acagtcaaag tgacggctaa
     5461 tgaaagaata ttgcaaaaaa atttagaaat tattttagat cctcaattgg ctgaatcgga
     5521 ttttactaaa aatttattga aagtctcgtt agaagaaaag gaaattaaag gtgaagacac
     5581 cttgagtact acagggatca gtttggttgg agataaagca cagggcaaag taaaattatt
     5641 taataaaact tttacggata aaactttttc agcaaacacg atctttaaat atgaagacat
     5701 tgcctttgtt ctaaatgaag aagtgacagt ccccgctgcc gtttccgaag aagaagacga
     5761 tgggcagaat attgattatg gtagtgttga agtggcgctg accgctcaag acattggggc
     5821 ggaaggaaat attggcaagg atagtaagtt agcggtagct gattttagtg aaaatactta
     5881 ctctgctaca gccattgaag atttttctgg tggctcaagt cgggaaatta gagtggtttc
     5941 tcaaaaagat ttagacaatt tacagaatca gttgaaagaa aatttgtatg cgattgctgt
     6001 aaaggaattt aaagaggagt ccaaagacgg agtttattac gtgccgactg gcgagagtcg
     6061 gatagtagat tttgcttacg atcatgaaga aggggatgag gtagaaactt tatcattaac
     6121 aatgaccttg gcagttgaag cagttaaata ctcatccagt gatttaaagc aattggcgga
     6181 agaagtctta ttgttagatt tgccggaaaa ttatgaattt gtagatgaag aaccatcttt
     6241 acttagcgat gaacctcagc ttatgaccga tggtagcaac cgtttagttc tagatgctga
     6301 attatcagcc aaggctaagg cgaagattaa ccaagaagca ttaaacgatc tagttttggg
     6361 taaaagttta gaagaagcca aaaataattt agaagaacaa gaaactattg atcgggctga
     6421 aataatttta caacctgctt ttatgggtcg agtattgcaa aaattaccaa aagattccag
     6481 ccgtttcaat ttcatcatta attaatatgg ctaaaacaaa tccgcaactg accaaacaaa
     6541 aaaaacagtt gttaaaactt tatcgccaag cactaagaga tggagtgcct atacaagact
     6601 tgaataaaaa agttaattac tttttgaaac aagataaagc cagtcagcag ggcgaaaaaa
     6661 atttagatga gcaaatccag ttaaattttt ggggccgctt acccaaaata gtgagagtgg
     6721 gggcaacttt tttgccattg gcattaatag tttttggctt ggcgttagct ttatctgcgc
     6781 tttggcccat tattgattat tatttaccat ggaaaaaaga agaagaaatt actttattat
     6841 ctccaatacc taattcgcaa ttactgttgg gcaatccatt aatttctgct caagctcaaa
     6901 ctgaaatagt agaaaatagc actaccgatt acagtgttga aactgaacca gtgattttag
     6961 atcaggatct tgattttaca gacctaacta attggtttag tgacgatcta gaaaacgtag
     7021 aattaagcac aagcgagttt attgaatata cgctagacat tcctcgttta gggattagta
     7081 atgccaaggt tcgagttggt ggaagtgatc ttaatcaaag tttaattcat tatcctggaa
     7141 ctggaatgcc tggacagttg ggtgctccag tgatatttgg tcattcggtt ttgcgtcaat
     7201 tttataatcc aagtgaaaac aatcctcgcc gttacaattc aatttttagt aaaataatga
     7261 ctctggaaaa aggtgacaaa atatatttaa cctatgacaa cgtcaaatat acctatgttg
     7321 ttcaaggcaa aactgaagtc aaagccgatg acacttttat tcttgctcaa cgagttgacg
     7381 ttcgtcaatt aaaattagtg acctgcgttc cagaaggaac taccttgcgt cgaggagtga
     7441 ttactgccca gttagtcaaa gaataaatta agaacatgtc aacaatttta gcaattgact
     7501 atggtttagc tcgaattggt ttagcgattt cctacgtggg tttggctgag ccactgacaa
     7561 tcatcaacaa tgatgtgacg agtaatgagc ccattattgg ttctcaagct ttagcagaaa
     7621 ttaagcaaat agtaaaagct gaaaaagttg acaaaattgt tttgggtatt tcagaacagg
     7681 caatggcaaa aaaaatctta gaattttttg agattttaca atcaacgttc aatttggatg
     7741 ttattttagt tgatgaagtt ttatcttcat atgaagttga gcagaggttg aaagaattgc
     7801 caaaaaagaa aagacgccag gcaatagacc actatgcagc agccattatt ctcgaaaact
     7861 acttagattt atgttagact aagcccctca atgaaaaatt ctccaaaaaa accacgttca
     7921 ttcttttatt tcttactaat ttttgtaatt attgttatct ctggatgggc aatttttatt
     7981 tcgttattac aagctccaaa tagcagtgat caaagtactg tcacttttat tattcccaag
     8041 ggtgaagcaa cgcaaaacat tgctcaacgc ctcaaagctg aaggtttgat tcggtctcct
     8101 ttagcttttc gtctcgccct ttatcaacag ggtaagcaaa ctagtattca ggcaggttct
     8161 tttaaattga gtccgagcat gtcagttagt gaattaaccc aagtattgac caaaggcacc
     8221 gatgatttat ggattacgat acctgaggga tggcggcgtg aagaaatagc tgctagttta
     8281 gttaaacaag acctta
//
LOCUS       DAOSEW010000024         8238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_7439_length_8238_cov_4.512526, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000024 DAOSEW010000000
VERSION     DAOSEW010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 8238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8238
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7439_length_8238_cov_4.512526"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1660)
                     /locus_tag="PLJ02_01450"
     CDS             complement(<1..1660)
                     /locus_tag="PLJ02_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOI05113.1"
                     /translation="MEKKFKSGVWQKQIDTRDFIFQNITPYDGDASFLVGPTATTKKL
                     WAKATQLLKKERENGGILDIDTETISTITSHKPGYLDKNLEKIVGYQTDQPLKRAIKP
                     FGGIRVVQAAAEENGYKISDEVVKIFTEYRKSHNDGVFDAYTEEMRKLRSTGILTGLP
                     DNYARGRIIGDYRRVALYGIDALIEAKKADKKALVGVMTDDLIRLREEVSEQIKALKA
                     MKVMASSYGYDISKPAETAQEAIQWTWFAFLAALKEQDGAAMSLGNVSSFFDIYLERD
                     LAAGKITEEEAQELVDHFIIQCRLIRHLRMNSYNELFAGDPTWTTECLGGFFSNGQHK
                     ITKTSFRFLQSLYNLGPAPEPNLTVLWSEKLPENWKKFCAQVSVDTSSIQYENDELMR
                     DKGNCDDYCIACCVSMMETGKEMQFFGARCNLAKAMLMAINEGRDEISGELVVPGIDP
                     LPAGPIDYAEFEKRFEKTISYLASAYVNTMNVIHYMHDKYYYEKAQLALMDTHCHRNM
                     AFGIAGLSVVADSIAAIKFAKVTPVRDKDGIAKDFKVNGDFPMFG"
     gene            complement(1818..3086)
                     /locus_tag="PLJ02_01455"
     CDS             complement(1818..3086)
                     /locus_tag="PLJ02_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05114.1"
                     /translation="MLIKKISFFLVIVALAVLSLVKFFDVSALASDQTLTLASDQAAT
                     IHIFSSKLCPHCREAEEFLRDLKEERPEITIYQYEVSYQQNSQLLALVANNLEVAGQG
                     VPFIVINNQVIVGFSEATPKAIDRLLSQEVPNLDLVLETVRANQLTPIIKELEMEEVA
                     PVVAEVPLTIDEQSEAIELPLFGKVQLKEVSLPLLTVMIGLIDGFNPCAMWVLIFLIS
                     LLIGMHDRRRMWILGSVFIFASGFVYFLFMSAWLNFFIFLGLVNWIRIAIAVLAIFVA
                     INYLRDFVKNKDGSCEVDLAGNKQKVFGRLKEVVYRKNLLWSLFGIILLAFSVNLLEL
                     VCSAGLPAVYTKILSMNELSTVQYYLYLLIYIVVFMLDDLAVFIITMITMKRVALNTK
                     YARYSHLIGGIVMLIIGLLMFFKPEWIMFG"
     gene            complement(3140..3952)
                     /locus_tag="PLJ02_01460"
     CDS             complement(3140..3952)
                     /locus_tag="PLJ02_01460"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HOI05115.1"
                     /translation="MSNHGNKQRALSIYEKQQLLKYGLSEAEFLKMGEMPVEYFTGRV
                     DFAGLTLEVNQDVLIPRIESEELVDQALDFAKSCPKQQLRILEIATGSGAIALALINK
                     IQQLNYLTKSWEFVLTEISAPALAVAKKNYRHLLAKDINQDKIKVKFILSNLLDEIKP
                     PPPFDLVLANLPYIPHSAIKSLGASVKEFEPPMALDGGETGFSLIASALEQLLEKQLL
                     ADEATVILETYHLHDQRFIRRNFSFLFTQFEIQFIKDQFNRQRFLHLKKISG"
     gene            4042..4839
                     /locus_tag="PLJ02_01465"
     CDS             4042..4839
                     /locus_tag="PLJ02_01465"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOI05116.1"
                     /translation="MAKSEKPNQFSFGNFISFINEHFALLIIILIVLTIGFVGGSLWT
                     ENQMLKSDNQVGVKDQAQKVEADNQIDDSLALDKIPELNAADHVLGAENADVIMISYL
                     DFQCSFCQRWHPTLNSLIEKYGDQITFAYRHFTLGFTYSDKLAQASECVAEYRGEEAF
                     WNFSNTLYEKLIDESIYTLENGNPIITDDSILTIAANAGASPNEVQACLESGEKAAVV
                     ETMKQEAVSAGIGGTPATIIISKKGGRELIGGALPIEEVEAMLEKHL"
     gene            complement(4892..6583)
                     /gene="recJ"
                     /locus_tag="PLJ02_01470"
     CDS             complement(4892..6583)
                     /gene="recJ"
                     /locus_tag="PLJ02_01470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOI05117.1"
                     /translation="MKKWQLQSKTEVQDIDQLLKVLLTNRQIKASQAADFFHPKPPQD
                     WQLKELGFDHAELIKIQKRLAIAKEKKEKVFIFGDYDSDGVCASAILWEGLQFLGINS
                     TPFIPNRQKHGYGLSVKALQDLFANQGKPDLLITVDNGIVALPALQFLAKEKVETIVT
                     DHHQKDQNKLPVLAVFHSTKICGAAVAWFLIAQLCKATDKPKLASKKLNELLSLVAIA
                     TVTDLMELVGINRSLVSHGLLALQQTERPGLLALYQLAGVNKEAINSYHLGYVIGPRI
                     NAMGRLAESMDALRLLCTKNPRQATQLANLLQDTNLARQDLTKDLINQVETSLTAKDK
                     QSKIIIVEGDYHEGVIGLLAGQLSEKYAKPCVVFAVPKDVHDQDLAIKASARSLIGLN
                     ITEFLRQVEDQLLSVGGHPLAAGFSVSLKNLASVKKRLMTLAKQQLADVALEAGINVD
                     CPISTKLVTLDTAQALEAFEPHGNANPRPIFLLKNLQLRNLQTLGKDAQHVKMFFTDE
                     KSGKEVVVLAWQFDQRFTKPTLGQNFNILIRLTVNQWRGSTKLQTTLVDLKNNAL"
     gene            6604..8217
                     /locus_tag="PLJ02_01475"
     CDS             6604..8217
                     /locus_tag="PLJ02_01475"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOI05118.1"
                     /translation="MQLNLPFESLFVIHALHEAGFEAYLVGGAVRDLILKQGDIQDFD
                     FTTNARPEEIQQVFAKSFYENDFGTVSITHDNLLEQMLSKKLWLPKNNLYQKLLKHDQ
                     EIYQKNQQEKIINLKKAKKIHFSLKERALASKKVIAQQKQLSILPPFEITTFRSDGDY
                     ADHRRPASVTWGKEIRDDLSRRDFTINAMAIAIDKNYLFQIFKENNQLQETYQLQPQH
                     WQLIDHFQGFQDLTNKLIRTVGNPNQRFREDALRMLRAIRLAVQLEMELEQATYQSIQ
                     KNKDLLKFISFERIGAEIMKILASAHPAEGIKLLDDTGLLEYIIPELKLGKKMDQPGH
                     HTTDVFVHSLDALAACPSRDPVVRLATLLHDIGKPETYKENDQEITFYNHEVLGARTA
                     SKIAKRLRLAKKDVQRIFILVRYHMFYYQPEQSDASIRRFIKKVGLENIDDILDLREG
                     DRLGSGAKKTSWRLEEFKERIIEQLNQPMDLTDLAVNGADLIKELKLKPSPVVGQILN
                     ELLDKVLQDPQLNTKKLLLKEAKQILQHK"
BASE COUNT         2698 a         1656 c         1382 g         2502 t
ORIGIN      
        1 tgccaaacat cggaaaatct ccattaactt taaagtcttt agctattccg tctttatctc
       61 taactggagt gactttggca aacttaatcg ctgcaattga atctgcgacc acactgagac
      121 cagcgatgcc aaatgccatg ttacgatgac aatgcgtatc cattaaagct agctgggctt
      181 tttcataata atacttatca tgcatataat gaatcacgtt catggtgttg acataagcac
      241 tagccagata gctgatggtc ttttcaaaac gcttttcaaa ttctgcataa tcgattggac
      301 cagccggtaa aggatcaata ccgggtacta ccaattcccc actgatctca tcacgacctt
      361 cattaatagc cattaacatt gccttagcca aattacagcg ggcgccaaaa aattgcattt
      421 ctttgccggt ttccatcatg gagacacaac aagcaataca gtagtcatca caattgcctt
      481 tgtcgcgcat cagctcatca ttttcatatt gaatcgatga agtatcaact gaaacttgag
      541 cacaaaactt cttccaattt tctggcagtt tttctgacca taaaacggtc agattgggtt
      601 ctggagctgg tcctaaatta tataaacttt gtaaaaatct aaaactggtt ttggtaattt
      661 tatgttgacc attactaaag aaaccaccca aacattcagt ggtccaagtg ggatcgccag
      721 caaataattc attgtaagaa ttcatgcgta agtggcggat tagtcgacac tgaataataa
      781 aatgatccac taattcctga gcttcttcct cagtaatttt acctgcagcc aaatctcgtt
      841 caagataaat atcaaagaaa gaactgacat tacctaaact catagccgcc ccatcttgct
      901 cttttagcgc tgctaaaaag gcaaaccaag tccactggat ggcttcttga gcagtttccg
      961 ctggtttaga aatatcataa ccataactgc tagccatcac cttcattgct tttagggctt
     1021 taatttgctc actaacttct tcccgtaatc taattaaatc atcagtcatc acccctacca
     1081 aggctttttt atccgctttt ttggcttcaa ttaaagcatc aattccatat agtgcgactc
     1141 ggcgataatc accaataatt ctgccacgag cataattatc tggtaaacca gttaaaattc
     1201 cagtagaacg cagttttctc atctcttcag tgtaggcatc aaaaacacca tcattatggc
     1261 tcttgcgata ttcagtaaaa atcttcacca cttcatcaga aattttatag ccattttctt
     1321 cggccgcagc ttgtactact ctaataccac cgaaaggttt aatggctctt tttagtggtt
     1381 ggtcggtttg gtaaccaaca attttttcta aatttttatc taaatagcca ggcttgtgac
     1441 tggtaatcgt agagatagtc tcagtatcga tatcaagaat gccaccgttc tctctttctt
     1501 tttttaagag ctgagtagct ttggcccata attttttagt ggttgcagtg ggacccacca
     1561 aaaaactggc atcgccatca tagggggtta tattctgaaa aataaaatct ctggtgtcaa
     1621 tttgcttttg ccaaacaccg cttttgaatt ttttttccat tctttccttc ttattgttat
     1681 tttttaatta atttcaaaca aaaaaataag gtctcatcga ctttacacga aaacatcata
     1741 atcaaattta tagctaaaaa caagcttttt tccgaaaatg gatctatgtt ataatacaaa
     1801 aactctcaac gagtcagtta gccaaacata atccactctg gtttaaaaaa catcaataaa
     1861 ccaataatca acatcacaat tcccccaatt agatgtgaat aacgagcata tttggtatta
     1921 agagccactc gtttcatagt gatcatggta atgataaata ctgccagatc atcaagcata
     1981 aacacaacaa tatagatcaa taaataaaga taatattgaa cagtgcttag ttcattcata
     2041 ctcaaaattt tcgtataaac cgccggtaaa ccagctgaac aaactaactc caaaagatta
     2101 acactaaaag ctaaaagaat gataccaaac agcgaccaaa gcaaattttt tcgataaacc
     2161 acttctttta agcgaccaaa aactttttgt ttattgcctg ctaaatccac ctcacaagac
     2221 ccatctttgt ttttgacaaa atctcgcaaa taattaattg ccacaaaaat cgctaaaaca
     2281 gcaatagcaa tccttatcca attgactaaa cccaaaaaga taaaaaagtt tagccaagca
     2341 ctcataaata aaaaataaac aaaaccagaa gcaaaaataa aaacggaacc taagatccac
     2401 atccgtcgcc gatcatgcat cccgatcagt aaagaaatta agaagattaa cacccacatg
     2461 gcacaaggat taaaaccatc tatcagacca atcattacag ttaataatgg taaagatact
     2521 tctttcagtt gcactttacc aaataaaggt agttctattg cttcactttg ttcgtcaata
     2581 gtgaggggta cttcagcaac aactggtgca acctcttcca tctccaactc cttaatgatt
     2641 ggagttaatt gattagctcg aacagtttct aggactagat ctaaatttgg cacttcctga
     2701 cttagtaatc gatcaatagc cttgggcgtg gcttcagaaa aaccgacaat cacctgatta
     2761 ttaatcacga taaatggcac accttgtcca gcaacctcta aattattggc gaccaaggct
     2821 aataactgag agttttgttg ataggaaact tcatattgat aaatagtaat ttccgggcgc
     2881 tcttctttta agtctcgcaa aaattcctct gcttcacgac aatgaggaca caactttgag
     2941 gaaaaaatat gaatagtagc tgcttggtca ctagcgagag taagtgtttg gtcactagcg
     3001 agtgctgaaa catcaaaaaa ttttactaaa gacaatacag ctaaagcgac aattactaaa
     3061 aaaaatgaaa tttttttgat tagcattctc ataaaacaag tgaggttgat tctacagctt
     3121 attaagtaaa gcttcaagct tagccactaa ttttttttaa atgcagaaag cgttgtctat
     3181 taaattgatc tttaataaat tgaatttcaa attgggtaaa caaaaaagaa aaatttcgcc
     3241 taataaatcg ctgatcatgt aaatgatatg tttcaagaat aactgtagct tcatcagcca
     3301 agagttgctt ctctagtaac tgctctaggg cactagcaat taaagaaaac ccggtttcac
     3361 ctccgtccag agccattggt ggttcgaatt ctttaacact ggcacccaaa cttttaattg
     3421 ccgagtgggg aatatatggt aaattagcca aaacgagatc aaatggtggt ggtggtttta
     3481 tttcatctaa taaattactc aagataaatt ttactttaat tttgtcctga ttaatgtctt
     3541 tggctaacaa gtgtcgataa ttttttttag ccacggctaa agcaggagct gatatttctg
     3601 ttaacacgaa ctcccagctt ttagttaaat aattgagttg ctgaatttta ttaattaaag
     3661 ctaaagcaat cgcgccactg ccagtagcaa tctcaagaat tcttagctgc tgcttcggac
     3721 aacttttagc aaaatcaagc gcttgatcga ccaattcttc actttcaatt cgtggtatta
     3781 acacatcttg attgacttct aaagtcaaac cagcaaaatc aacgcggcca gtaaaatatt
     3841 caactggcat ttctcccatc tttaaaaatt cagcttcact caagccatat tttaacaatt
     3901 gttgcttttc atagatcgat aacgctcgct gtttattgcc gtgattggac ataaaaatta
     3961 ctcaatggtt tttttaaaac ttatgctaga ataatcgcta ttattgttag aataaccgct
     4021 attatagaga tgaaaggcta aatggcaaaa tcagaaaaac caaatcaatt ctcctttggc
     4081 aattttatca gctttatcaa tgagcacttt gccctactta tcattatttt aattgtcctt
     4141 actatcggtt ttgttggcgg ttcgctgtgg acagagaatc aaatgttgaa atctgataat
     4201 caagttggtg taaaagatca agctcaaaaa gtagaggcag acaatcaaat tgacgattcg
     4261 ctggctttgg acaaaattcc tgaacttaac gcagctgatc acgtacttgg cgccgaaaat
     4321 gctgatgtaa tcatgatttc atacctagat tttcaatgct ctttttgtca acggtggcac
     4381 cccactttaa acagtttaat tgaaaaatac ggtgatcaaa tcacttttgc ctatcgacat
     4441 tttaccttag gctttactta ctcagacaaa ttagctcaag cgagtgagtg tgttgctgaa
     4501 tatcgcggtg aagaagcctt ttggaacttt tccaataccc tttatgaaaa gttaattgac
     4561 gaatcaattt acaccctaga aaatggtaat ccaattatta ctgatgacag tattttgact
     4621 atcgccgcta atgctggtgc ttcgccaaac gaggttcaag cgtgtcttga aagtggcgaa
     4681 aaagctgctg tagttgaaac aatgaaacaa gaagcggtta gtgccggaat tggtggcact
     4741 ccagcgacaa tcatcatcag caaaaaaggt ggtcgagaat taattggtgg cgccctaccc
     4801 attgaagagg tagaagccat gttggaaaag catttataaa aaattatttt tctctacgac
     4861 aaattgctgt gtctataaag cattgttttt tctataaagc attgtttttc agatcaacta
     4921 aagttgtttg aagcttggtg ctgcctcgcc actgattaac tgtcaatcta attaaaatat
     4981 taaaattttg gcctaaagtc ggcttagtaa atctttgatc aaattgccaa gctaaaacaa
     5041 ccacttcttt gccacttttt tcgtccgtaa agaacatttt gacatgttga gcatctttgc
     5101 ccagcgtctg caaatttctt aattgcaaat tttttaataa aaaaattggt cgagggttag
     5161 cgttaccatg cggttcaaaa gcttctaaag cctgagcggt atccagcgtc actaatttag
     5221 ttgaaatagg acaatccaca ttaataccag cttctaaagc cacatcggcc aattgttgtt
     5281 tagctaaggt cattaaacgt tttttgactg aggctaaatt ttttaaactt accgaaaagc
     5341 cagccgctaa aggatgaccc cccacactta ataattgatc ctcgacttga cgtagaaatt
     5401 cagtaatatt taaaccaatt aatgatcggg cagaagcctt gatagctaaa tcttggtcat
     5461 gaacatcttt gggaacggca aaaacaacac agggtttagc gtatttttca ctcagttggc
     5521 cggctaaaag gccaatcact ccttcatggt aatcaccttc gacaattatt atttttgatt
     5581 gtttatcttt ggctgtcaat gaggtttcaa cctgattaat caggtctttg gtcaaatctt
     5641 gtcgtgctag atttgtgtct tgtaacagat tggctaattg tgtagcttgt cggggatttt
     5701 tagtgcacaa caaacgcaac gcatccatac tctctgccaa acgccccatc gcgttaattc
     5761 taggaccgat gacataaccc aaatgataac tattgattgc ttctttgttg actccagcta
     5821 actgataaag cgctaataaa cctggacgct ctgtctgttg taaagctaac aaaccatgac
     5881 taactaaact acgattaatt ccaactaatt ccatcaaatc agtaacggtg gcaattgcga
     5941 ccaaagacaa taactcattt aattttttac tcgctagctt gggtttatca gtcgctttac
     6001 ataattgggc aatcaaaaac caagccactg ctgctccgca aatttttgtt gaatgaaaaa
     6061 cggctaaaac tggtaattta ttttgatctt tttggtggtg atcagtaact attgtctcta
     6121 ctttttcttt agctagaaac tgcaaagctg gcaaggctac aatgccatta tcaacagtga
     6181 ttaataaatc tggtttacct tgattggcaa aaaggtcttg caaggctttg accgataaac
     6241 cataaccatg tttttgccga ttgggaataa aaggggtgct attaattcct aaaaattgca
     6301 aaccttccca taatatggcg ctagcacata caccatcgct atcataatcg ccaaaaatga
     6361 aaactttttc ctttttttcc ttagcaatgg ccaatctttt ttgaatcttt atcaactcag
     6421 cgtgatcaaa acctaactct tttaattgcc aatcttgtgg gggtttgggg tgaaaaaagt
     6481 cagctgcttg gctcgcttta atttgccgat ttgtcaataa tactttaagt agttgatcga
     6541 tgtcttgaac ctcagtttta gattgcaact gccatttctt catagttctt ttataataac
     6601 cttatgcagt tgaatttacc atttgaatca ctttttgtca ttcatgccct tcacgaagct
     6661 ggttttgagg cttatttagt aggtggtgca gtccgagatt taatcttaaa acaaggcgat
     6721 attcaagatt ttgatttcac cactaacgcg cgaccagaag agattcaaca ggtgtttgcg
     6781 aaaagttttt atgaaaatga ttttggcaca gtcagcatca cccatgacaa cttacttgaa
     6841 caaatgttga gcaaaaaatt atggctaccc aaaaataacc tctatcaaaa attattaaaa
     6901 catgaccaag aaatttacca aaaaaatcaa caagaaaaaa tcattaattt aaaaaaagca
     6961 aaaaaaattc atttttcatt aaaagaaaga gctttagcct ctaaaaaagt tattgctcaa
     7021 caaaaacagt tatctatctt acctcctttc gaaattacca cttttcgcag tgatggtgat
     7081 tacgccgatc atcgtcgccc agcctcagtt acttggggaa aagaaattag ggacgatttg
     7141 agtcgtcgcg attttacgat taacgctatg gccatagcca ttgataaaaa ttacctattt
     7201 caaatattca aagaaaacaa ccagcttcag gaaacttacc aattacaacc acaacactgg
     7261 caattgattg atcattttca gggctttcag gatctaacca acaaattaat cagaacagtg
     7321 ggcaatccca atcaacgctt tcgtgaagac gccttaagaa tgttgcgggc catccgtttg
     7381 gctgtacaac tcgaaatgga actggaacaa gcaacttatc agtcaattca aaaaaacaag
     7441 gacctactaa aatttatcag ttttgaacgc attggtgcgg aaataatgaa aattttagcc
     7501 tcagctcatc ccgctgaagg tattaagcta ctagatgaca ctgggctatt agaatatatt
     7561 atccccgaat taaaactggg aaaaaagatg gatcagccag gccatcacac caccgatgtc
     7621 tttgtccata gtttagatgc cctagccgcc tgtcctagcc gtgatccagt ggtgcgctta
     7681 gccaccctcc ttcatgacat cggtaaacca gaaacttata aagaaaatga tcaagaaatt
     7741 actttttata atcatgaagt tttaggggct agaaccgcta gcaaaattgc caaaagactc
     7801 cgtctagcca aaaaagacgt acagcgaatt tttattttag ttcgctatca catgttttat
     7861 tatcagccag aacagagtga tgcttcaatt agacgtttta tcaaaaaagt cggcctagaa
     7921 aatattgatg atattttaga tttgagagaa ggtgatcggc ttggtagcgg agctaaaaaa
     7981 actagttggc gtctcgaaga attcaaagaa cggattattg agcaattaaa tcaacccatg
     8041 gatttaaccg atttagccgt taatggagca gatttaatta aagaattaaa gctcaaacca
     8101 agcccggtgg ttggtcaaat tcttaacgaa ctcttagata aagtattgca agatccacaa
     8161 ttaaatacca aaaaattatt attgaaagaa gcgaaacaaa ttctacagca caaataatag
     8221 actatttgtg cttttgac
//
LOCUS       DAOSEW010000025         8186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_7503_length_8186_cov_4.120280, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000025 DAOSEW010000000
VERSION     DAOSEW010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 8186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8186
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7503_length_8186_cov_4.120280"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..496)
                     /locus_tag="PLJ02_01480"
     CDS             complement(35..496)
                     /locus_tag="PLJ02_01480"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOI05119.1"
                     /translation="MCVAVSYITYVVKSVKPPARLKRKTFSKKLLNNSLIKQGCFAES
                     IACAFLTKNNYQILAQNLAYKNHELDIVALDLQYDEYVFVEVKWRNSDRFGEGSQAVD
                     YKKILSMQIVAKQWLAKQKFPKAWRFDIISLLGNLKSRNLTIRHYKNISWL"
     gene            complement(399..746)
                     /gene="rplS"
                     /locus_tag="PLJ02_01485"
     CDS             complement(399..746)
                     /gene="rplS"
                     /locus_tag="PLJ02_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006068225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOI05120.1"
                     /translation="MAISFTYHEQNINIGDSVRVHQEIVEGEKKRIQIFEGIVIAIKN
                     RELNKSFTVRKIAANNIGVEKIFPVAMPSIKKIEVKRQGNVRRSKLYYLRGKVGKAAS
                     KVKEKNLFEKVTQ"
     gene            complement(801..2516)
                     /locus_tag="PLJ02_01490"
     CDS             complement(801..2516)
                     /locus_tag="PLJ02_01490"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012268893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOI05121.1"
                     /translation="MVDIRGFLQQAAQKHPTKYVFVSGGVLSGLGKGITAASLGVVLK
                     AHGYSVTNVKCENYLNLDSGNINPVEHGDVFLCDDGLEADLDLGSYERFLGEEVGYKN
                     FITMGQVYSTVIENAKNLKYEGITVDAIPYVPEEVIRRIQAAGKGYDICLVELGGTVG
                     EYQNIIYYEAYRIMKMSLPDDLIIIHVTYFPNPSHINELKSKPTQLSVKTLNSMSIQP
                     DFIVGRGATYIDDKRKEKVAYFCNMEKEEIISNPDCKSIYEIPLIFKEQHFGEKVLKK
                     LKLANKKIQLNDWQKMVEKIYSQKKKQISIAIVAKYIKSGDFELKDSYTSLLEAIDHA
                     AWQNEVEVKINFINAEKIEKNDKVSLAKLKKAEGIIVPIGWGKRGVEGKIKAIQYARE
                     NKIPYLGLCYGMQLASIEFARNVVGLKEANTEEVDPKTKYPIIHAIPFDKKYQKIKST
                     EASMRLGAYDCVLKKGTLAYEIYKKHHAFKDEKKGLISERHRHRFEFNNEYRQILEEH
                     GFVISGTSPDNFFVEMIELPQKDHPFFLATQAHPEYKSRPLTAHPIFIEYLAAILKQA
                     PEKKK"
     gene            2585..3154
                     /locus_tag="PLJ02_01495"
     CDS             2585..3154
                     /locus_tag="PLJ02_01495"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOI05122.1"
                     /translation="MKKIYSKTGDDGQTALINGERLAKDSLVFEVLGTLDEFNASLGV
                     LLANWQETFSPKTGLTQTKDIALQKRFLLDLQKQLFKLGAEIAKSPSVKLQNSFLERM
                     EKNIDQLQNQLGKDFWSNFVLPGGSQLAAQVDLARTICRRLERRLVALDAERDLRDIL
                     IKFVNRTSDYLYILRSYINHELKVKEIPV"
     gene            complement(3160..3780)
                     /gene="lexA"
                     /locus_tag="PLJ02_01500"
     CDS             complement(3160..3780)
                     /gene="lexA"
                     /locus_tag="PLJ02_01500"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOI05123.1"
                     /translation="MAVTLYKRQRQIVDFIAQYIQKNGYAPTLKEIADSIGVSSLATV
                     HEHLQALQRKKIIRKHEGAVRGIELIDRTFLNITDSVDLPFLGYIAAGAPIEPHEDPE
                     ASFKVSPEMISGKKRAFVLQVKGKSMIEDHIDDGDYVVIEETPEVNNGDIVVALLDNG
                     LATLKRFYKEVTRIRLEPANSTMSPIYATNVQIQGRVVGLIRKFRY"
     gene            3951..4625
                     /locus_tag="PLJ02_01505"
     CDS             3951..4625
                     /locus_tag="PLJ02_01505"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOI05124.1"
                     /translation="MSGITDFILNFLTNFANSVSLPLFVIVGALTEEVLAVIPSPFVP
                     LTAGSLAYNQGKSVYFILFLVLIGAIAKTFSTSVVFWFSDKLEDLITKGKLGKILGLE
                     ANEIERYGKIFSKNKSNIFIVFILRALPFIPTLPVTVVSGLIKVKYRIFALGTFLGVL
                     VRNSFYLLGAFYGLQKFQSLVEGVDALSSIFEILMIIGFLGFIFFLLRNNWERIFMAL
                     KKGKKK"
     gene            complement(4626..5882)
                     /locus_tag="PLJ02_01510"
     CDS             complement(4626..5882)
                     /locus_tag="PLJ02_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HOI05125.1"
                     /translation="MAKIYALQAKEVFDSRGIPTIELLLWLDNGFSTITTVPTHTDKN
                     QFDAIALRDNDERFLGLGVAKAVNHINQIIAPQIIEKEVTAQGELDKILLELDGTANK
                     SRLGANALLAVSQAILKAGAISVGLPLYEYIQKKYNLIQEFVLPTCIVTAFDGGSHGG
                     KNLDFQEFSLIPASHLSLEKSLEMIAVIKQKLETLLINKGAIHATGLTGGFTPNLYKN
                     LDAFELLVEAIRTTTYNFAQDVFFGLQAAAYHFFEASKYRIRDANQGFTEEELLQFYE
                     QVREKYKIIYLEDPFVENDKKAWQMLNRDIGQTTTIAACRHVAAKVSEINKIKNDEMA
                     NAVVLKPIQIGTISELMESIKTVKDHKLDLIISHSTGETLETLIVDLAVGVGANYVKF
                     GPLNRGERIVKYNRLLDISRELNSKK"
     gene            complement(5940..7031)
                     /locus_tag="PLJ02_01515"
     CDS             complement(5940..7031)
                     /locus_tag="PLJ02_01515"
                     /inference="COORDINATES: protein motif:HMM:NF021916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HOI05126.1"
                     /translation="MTDVKDAAILDNRTAISKLDPDGALASAESLADQINQVWQERQQ
                     IKFAENEIKSIANIVVAGMGGSALGADVVWHLFKDQLTVPMYINRDYQLPNFVDHNTL
                     VILSSYSGDTEEILSCAKLAEEKKAKIAVISSGGKLADIAKNNNYPSFFINAKHNPSQ
                     QPRMAIGYSILAFICVLSAAKLLDFQEKQLLEIIDVVNQVTDKVKIEIDQANNQAKLL
                     AYLSLERRPILVGAEFLEGALHTACNQFNENAKIYADYKIIPEINHHLLEGLNFPKSN
                     SLNHFFIFFQSQLYHPRNQKRIELTQKLLEKKDIDNIMVNLESDNKITQVFELITLTT
                     FANLYLAFLEKINPCPIPSVDWFKEQLKK"
     gene            complement(7024..7545)
                     /locus_tag="PLJ02_01520"
     CDS             complement(7024..7545)
                     /locus_tag="PLJ02_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOI05127.1"
                     /translation="MTNHYFIDNYHYCPRCKSQLTKLTTHLHCANCALKIYPNPSLAV
                     ALVAFNQKGEVLLNKRKIAPNPGSWDTVGGFVDVGETVEEAIKREFHEETRGSCEIVK
                     YWGSYPDVYGVEQSPTINLFFEVAIGDEKLKASDDAAELRFFPLQQLPQNLAFANTQV
                     FLDLLIKEKGKND"
     gene            complement(7551..>8186)
                     /locus_tag="PLJ02_01525"
     CDS             complement(7551..>8186)
                     /locus_tag="PLJ02_01525"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOI05128.1"
                     /translation="IVSLFAKDVLDFLPGSKIIYNTLCSRQVQETIEQAQGKGVMWLT
                     GHSFIKAKVKEERSPFGGELSGHIFFMDNFYGHDDAAYACLRLLSYLERRKIKLSEAV
                     SELVQYISSPEIKLGLADEIKFQLIDQEITADFKKHWPQAKFTKIDGIRMDLPEQMAI
                     IRASQNGPYVTVKFEGKTKEQYQTLKKEIRNILKQYPAVNWQEGTNVHALD"
BASE COUNT         2488 a         1503 c         1413 g         2782 t
ORIGIN      
        1 cgatgtccta accgctagac gatagggcca agttttataa ccaactaatg tttttatagt
       61 gtctaattgt taaatttcga ctttttaagt ttcctaacaa ggatatgata tcaaatcgcc
      121 atgcttttgg aaacttttgt ttagccaacc attgtttagc aacaatttgc atcgacaaga
      181 tttttttata atcaactgct tgacttcctt cgccaaatcg atcgctattt ctccacttca
      241 cttccacaaa aacatattca tcatattgga gatctagggc aacaatatct aattcatgat
      301 ttttgtaggc aagattttgc gccaaaattt gataattatt ttttgttaaa aatgcacaag
      361 caattgattc agcaaaacaa ccttgtttga tgagtgaatt attgagtaac tttttcgaaa
      421 aggtttttct ctttaacctt gctggcggct ttaccgactt taccacgtaa gtaatataac
      481 ttactgcgac gcacatttcc ttgacgttta acttcaattt ttttgatact aggcattgct
      541 acgggaaaga ttttttctac gccaatattg ttagcagcaa tcttgcgcac tgtaaaactt
      601 ttatttaatt cacggttttt aatagcaatc actatacctt caaaaatctg aattcgcttt
      661 ttttcacctt cgacgatttc ttgatggacc cggaccgaat caccgatgtt tatattttgc
      721 tcgtggtatg taaaactaat tgccataata agcgggtatt gtagcagagt tagcaccgct
      781 ttggcaagca gaaaagacgt ttattttttc ttttctggtg cttgttttaa gatggctgct
      841 aagtattcaa taaaaatagg atgagctgtt aacggtctag atttgtattc tggatgagcc
      901 tgagtggcta aaaagaaagg atgatccttt tgaggcaact cgatcatttc cacaaaaaaa
      961 ttgtcaggac tagttccaga aataacaaaa ccatgttctt caaggatttg ccgatactca
     1021 ttattaaatt caaaacgatg gcgatgtctt tctgaaatta atcctttttt ttcgtcttta
     1081 aatgcgtgat gttttttata gatttcatag gctaaagtgc cctttttgag tacacaatca
     1141 tatgcgccca gtctcatgct ggcttcagtg cttttaatct tttgatattt tttatcaaaa
     1201 ggaatagcgt ggataattgg atatttagtt ttgggatcaa cctcttcagt attagcttct
     1261 tttagaccga ccacattgcg cgcaaactca atcgaagcca actgcatgcc gtaacacaaa
     1321 cctaagtaag gaattttatt ttcccgcgca tattgaatgg ctttaatctt accctcaact
     1381 cctcttttgc cccaaccgat gggaacaata atgccttcag ctttttttaa ttttgctaat
     1441 gagactttat catttttctc aattttttcg gcattgataa aattgatttt tacctcaact
     1501 tcattttgcc aagcagcgtg atcaatggct tctaagagtg aagtatatga gtcttttaat
     1561 tcaaaatcac cacttttaat gtacttggca acgatggcaa tcgaaatttg ttttttcttc
     1621 tgactataga ttttttctac catcttttgc caatcattaa gttggatttt tttattggcc
     1681 agtttcagtt ttttcaatac tttttcgccg aaatgctgtt ctttaaaaat taagggaatt
     1741 tcataaattg atttgcaatc aggattagaa ataatttctt ctttctccat attgcaaaaa
     1801 taagctactt tttcttttct tttgtcatca atataggttg ctcctcgacc cacaataaaa
     1861 tcaggttgaa tactcataga attaagagtt ttaactgata attgagtcgg ttttgatttc
     1921 agttcgttaa tgtgactagg attaggaaaa taagtgacat gaataataat caagtcatcg
     1981 ggcaaagaca ttttcataat ccgataggct tcgtaataaa tgatattttg atattcacca
     2041 acagttccgc ccaattcgac caaacagata tcatagccct tgccggcggc ctgaatgcgt
     2101 ctaataactt cttctggtac ataaggaatg gcgtcaactg ttataccttc gtatttgagg
     2161 tttttggcat tttcgatcac cgtactgtag acctgaccca tggttataaa gtttttataa
     2221 ccaacctcct caccaaggaa tctttcatac gatcctaaat ctaaatcagc ttctaaacca
     2281 tcatcgcaga gaaacacgtc gccatgttca actggattaa tattgccaga atctaaatta
     2341 agataatttt cacatttaac attagtgaca ctgtaaccgt gggcttttag tactacaccc
     2401 aaggacgctg cagtaatgcc cttgcccagt ccagacaata ctccaccgga aacaaaaaca
     2461 tatttagtgg gatgcttttg agcagcttgt tggaggaatc cgcgaatatc taccatcacg
     2521 ggattttatt atagataaat tttagctatt tgaaaagaca taaaacaaat tagaatagtg
     2581 gtgtatgaaa aaaatttata gtaaaactgg agatgatgga caaaccgcac taattaatgg
     2641 tgaaagatta gccaaagaca gcttggtttt tgaagtttta ggtaccttgg atgaatttaa
     2701 tgccagttta ggggttttat tagcgaattg gcaagaaaca ttttctccta aaacaggctt
     2761 aacacaaacc aaagatattg ctttacaaaa aagatttttg ttagatttgc aaaaacagct
     2821 ttttaaatta ggcgctgaaa ttgccaaatc gcccagcgtc aaattgcaaa atagtttttt
     2881 agaaagaatg gaaaagaata ttgatcaatt acaaaatcaa ttaggtaaag atttctggag
     2941 caattttgtc ctgcctggag ggagtcagtt ggccgctcag gtggatttgg cgcgcaccat
     3001 ttgtcgcaga ttggaaagaa gattggtggc tttggacgcc gagcgggatt tacgagatat
     3061 tctgattaaa tttgtaaacc gcactagcga ttatttatat atcttaagat cgtatattaa
     3121 tcacgaactt aaagtcaaag aaataccagt ttagactctt tagtagcgaa acttgcgaat
     3181 taaacctact actcgaccct gaatttgcac gttagtggca taaattgggc tcatggtgga
     3241 gttggctggt tctaatctaa tgcgagtgac ttctttatag aaacgtttta aagtagctag
     3301 accgttatct agtagagcca cgacaatgtc accattgttt acctcgggag tttcctcaat
     3361 caccacatag tcgccatcat caatatgatc ttcgatcatt gatttgcctt taacctgcaa
     3421 cacaaaagct ctttttttac cagaaatcat ttcgggagag actttgaaac tagcctcagg
     3481 atcttcgtgg ggttcaatgg gagcaccagc ggcaatgtaa cctaaaaatg gcagatcaac
     3541 actgtcagtg atgtttaaga aggttctatc aatcaactca atacctctaa cggcaccttc
     3601 atgttttcga ataatttttt tacgctggag cgcttgtagg tgttcgtgaa cggttgccaa
     3661 agaagaaaca ccaatagaat cagcgatttc ttttaaggtt ggtgcataac catttttttg
     3721 aatatattga gcaataaagt cgacaatttg tctttgtctt ttgtataaag taactgccat
     3781 agacccgata ctttcactaa ctaataacga ataataacca taaaataaac gaatttctcc
     3841 caaaacacaa ctagtaaatt atatatcata atatatcaaa gattattaaa gatcatcaat
     3901 taaaaattgc tactgactta ttaaaaaagt tcggctatac tttttaggtt atgtcaggaa
     3961 ttaccgactt tattttaaat tttttaacta attttgccaa ttctgtttca cttcccctct
     4021 ttgtgattgt cggtgcgtta accgaagagg tcctggcagt gattccgtcc ccctttgtgc
     4081 ccctcactgc cggttcttta gcctacaatc aaggaaaatc tgtttatttt attcttttct
     4141 tggtcttaat tggtgcgatt gctaagactt tttctacctc agtagttttc tggttttcag
     4201 acaaattaga agatttaatt actaagggta aactcggtaa aattttaggt ttagaagcta
     4261 atgaaattga acgttacggt aaaatttttt cgaaaaacaa aagtaatata ttcattgttt
     4321 ttattcttcg ggctctgcca tttattccaa cgttgccagt gacagtggtt tctggtttga
     4381 ttaaagttaa atatcggatt tttgcactag gtactttttt gggggtgtta gtgcgtaata
     4441 gtttttatct tttgggggct ttttatggat tgcaaaaatt tcagagttta gttgaaggcg
     4501 tagatgcttt aagttcaatt tttgaaatat taatgattat tggttttttg ggttttatct
     4561 tttttctatt acgcaacaat tgggaacgca tttttatggc cttaaaaaaa gggaaaaaga
     4621 aataattatt ttttactgtt taattctcga ctaatatcta acaaccggtt gtatttaacg
     4681 attctctctc ctctattgag tggcccaaat ttgacataat tagcgccaac tcccacagcc
     4741 aagtcaacaa ttagagtttc taaagtttca ccagtactat gactgatgat taaatcaagc
     4801 ttatgatctt taacggtttt aatactttcc atgagctcac tgatggtgcc aatttggata
     4861 ggtttgagta caaccgcatt agccatttcg tcatttttaa ttttgttgat ttctgaaact
     4921 ttggccgcaa catgacggca agcggcaata gtagtggttt gaccaatgtc ccgattgagc
     4981 atttgccaag cttttttatc attttctaca aacggatctt ctagataaat aattttatat
     5041 ttttcacgca cttgttcata aaattgaagc aattcttctt cagtaaaacc ctgattagca
     5101 tcacgaattc gatatttact ggcttcaaaa aagtgataag cagctgcttg taaaccaaag
     5161 aaaacgtctt gagcaaagtt ataggtggtg gttctaatag cttctactaa taattcaaaa
     5221 gcatctaaat ttttatataa attgggtgta aaaccaccag taagaccggt agcatgaatg
     5281 gcacccttat taattaataa agtttccaac ttttgtttaa taacagctat catttccagc
     5341 gatttttcta aagatagatg gctagcagga atcagggaaa attcttgaaa gtccaagttt
     5401 tttccgccat gactaccacc atcaaaagcg gtgacaatac aagtgggtaa aacaaattct
     5461 tgaatcagat tatatttttt ttgaatatat tcatagagcg gtaaaccaac actaattgca
     5521 ccagctttta aaatggcttg agacactgcg agaagagcgt tagcgcccaa gcgactttta
     5581 ttagctgttc cgtcaagttc taataaaatt ttgtctaatt ctccctgagc cgtgacttct
     5641 ttttctataa tttggggagc aataatttga ttaatgtgat tcactgcctt agcaacaccc
     5701 aaacctaaaa accgctcatc attgtctctc aaagcaatcg catcaaattg atttttatca
     5761 gtgtgcgtgg ggacggtcgt aatcgttgaa aaaccattgt cgagccacaa taataattca
     5821 atagtcggta tgcctcgact atcaaacacc tctttagctt gaagagcata aattttagcc
     5881 ataaattaat attctcagta tatctaattt tgttctgctt tgaaagactt gtaaaattat
     5941 tattttttta attgctcttt gaaccaatca acactaggaa ttgggcaagg attaattttt
     6001 tctaaaaagg ccaaataaag attggcaaat gtagttagtg taattaattc aaaaacttga
     6061 gtaattttat tatcgctttc taaattgacc ataatgttat caatatcctt cttttccaaa
     6121 agtttttgcg ttaattcaat ccgcttttga tttcttgggt gataaagctg tgactgaaag
     6181 aatataaaaa agtgatttaa cgagttgctt ttaggaaaat ttaatccttc caaaagatga
     6241 tggttaattt ctggaataat tttataatcg gcataaattt tggcgttttc gttaaattga
     6301 ttgcaggccg tatgcagcgc tccctctaag aattctgccc ccaccaaaat gggtcttctt
     6361 tctaagctta aataagctag aagtttggct tgattgtttg cttgatcaat ttcaattttt
     6421 accttgtcag ttacttgatt cactacgtcg ataatttcta acaattgttt ttcttgaaaa
     6481 tctaacagtt tcgctgccga taaaacacaa ataaaagcca agattgaata gccaatggcc
     6541 attcgtggtt gttgtgaagg attgtgtttc gcattaataa aaaaacttgg gtaattattg
     6601 tttttggcaa tgtccgctaa tttacctcct gaactaatca ctgctatctt agcttttttt
     6661 tcttcagcca gtttagcgca cgacagaatt tcttcagtgt cacccgaata actcgataaa
     6721 atgactaagg tgttgtgatc gacaaaatta ggtaattgat aatcacgatt aatatacatt
     6781 ggtacggtca attgatcttt gaataaatgc catacgacat ctgcgccaag agcactgcca
     6841 cccatgccag caaccacgat attggcaatg gatttgattt cattttcagc aaacttaatt
     6901 tgctgtcttt cttgccaaac ttgattgatt tgatcagcta aactttcggc actagctaaa
     6961 gcaccatcgg ggtcaagttt actaatggcg gtgcgattat ccaaaatagc agcatcttta
     7021 acgtcagtca ttttttcctt tttctttaat taataagtca agaaaaactt gagtattggc
     7081 aaaagctaaa ttttggggta gttgctggag cggaaaaaaa cgcaactctg cagcatcatc
     7141 actggctttt aatttttcat caccaatggc tacttcaaaa aacagattaa tggttggtga
     7201 ttgttcaaca ccataaacgt ctggataaga accccaatat ttaacaattt cacaactacc
     7261 ccgagtttcc tcatgaaatt ctcttttaat ggcttcttca acggtctcac caacatcaac
     7321 aaaaccaccg accgtatccc agctaccggg attgggggca attttgcgtt tattcaataa
     7381 cacctccccc ttctgattaa aggcaactaa ggccactgcc aaagaaggat tggggtaaat
     7441 ttttaaagcg caattggcac aatgtaaatg agttgtcaac ttagtgagtt gcgatttaca
     7501 gcgaggacaa tagtgatagt tatcaatgaa gtagtgatta gtcataatta ctaatctaaa
     7561 gcgtgaacgt ttgttccctc ctgccagttg actgctggat attgctttaa gatatttctt
     7621 atttcttttt taagggtttg gtattgttcc ttggttttcc cctcgaattt aacagtgaca
     7681 tatggtccat tttgagacgc acgaatgata gccatttgtt ccggtaggtc catacgaatc
     7741 ccgtcgattt tagtaaattt agcttgtggc caatgttttt taaaatcagc ggtaatctct
     7801 tgatcaatta attgaaattt aatttcatca gctaaaccta acttaatttc tggactacta
     7861 atatattgga ccaactcact gactgcttca cttaacttga tttttcttct ttctaaataa
     7921 ctcagtaatc gtaaacaagc ataagcagcg tcatcgtggc cataaaaatt atccataaaa
     7981 aagatgtgac cagataattc tccaccgaaa ggtgaacgct cctctttcac tttagctttg
     8041 ataaaagaat ggccagtaag ccacatcact cctttgccct gagcttgctc aatggtttct
     8101 tgaacttgac gagaacagag agtgttataa ataattttac ttcctggtag aaaatctaaa
     8161 acgtctttag caaacaacga gacaat
//
LOCUS       DAOSEW010000026         7899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_7908_length_7899_cov_4.214814, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000026 DAOSEW010000000
VERSION     DAOSEW010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 7899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7899
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7908_length_7899_cov_4.214814"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="PLJ02_01530"
     CDS             complement(<1..682)
                     /locus_tag="PLJ02_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05129.1"
                     /translation="MSEETKETKQLKTLSHYFDDYIKQYQMDWEYRHQPVIAALEVDE
                     VASKIAKAYEKVREVVDWKEENLLRRAAIERILKRRLVSKLYGISIMPDVKAKEIAEP
                     MALELMRSGYFDRGRIGRKQVEELENILNKYIKIMTDSRLPRSNDDIQVKKRINFYNW
                     IIEVAACEIEELLFPNFKENALLNLMTNVLYNRIKIIPGNSVSDMEKFLQTYISVHRT
                     LYNFDNPII"
     gene            738..1613
                     /locus_tag="PLJ02_01535"
     CDS             738..1613
                     /locus_tag="PLJ02_01535"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOI05130.1"
                     /translation="MADKYQATWVSHSSISDFLQCPRAYYLKNVYKDPKTKHKIQLIS
                     PPLALGAAVHSVIEALSVIPAEQRFAESLIKKFDQEWQKFTGIKGGFFDEATEMAYKE
                     RGAEMLRRVMNNPGPLTDLAVKIKEDLPWFWLSEEEEIILCGKIDWLEYLPETDQVHI
                     IDFKTGKNDEDENSLQLPIYYLLVHYCQHRAVAKASYWYLESSDEPVEQELPDLAWAE
                     AEVLAIAKKIKTVRKLEHYKCPQGENGCWACRPMEQILKGEAQLVGENDYQQDVYVLP
                     SKQKKIARKDNSRLI"
     gene            complement(1610..2857)
                     /locus_tag="PLJ02_01540"
     CDS             complement(1610..2857)
                     /locus_tag="PLJ02_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05131.1"
                     /translation="MKVKTFSYHLLVFLLISFSFLSFKTNPALAQDCDQEFKCDELKE
                     TDTDQYSNCLNDQRRCWESKVNQAKSEANTLQSAINILNGQIQLQSIKIQQTTNEIYQ
                     LEKEINELTQRIEGLSISLNKLSEILIERVRASYKQSRKQYKVNFFASDSFNDFITQY
                     RYINQAQEQTLEIMHRTELQRATYDQQKQLKEEKQAEVNLKKSELERQKAELDAQKSV
                     KNKLLAETKSSETIYQQKLAEAIAELNSLKAFSSSKSGSVLPEQNSPDGWFFSQRDQR
                     WANQLIGSSNMSIMEVGCLISSTAMIKKKFGENVNPITISNNSSYFFSNTAYMSRPWP
                     TPAGYYYQYNTYSQSTLDSKLNENPVIVKLSAGPYGSHFIVIKEKRGDQYIMHDPWEG
                     YDKNFSDFYSLSQIQQIASLVKN"
     gene            complement(2863..3732)
                     /locus_tag="PLJ02_01545"
     CDS             complement(2863..3732)
                     /locus_tag="PLJ02_01545"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOI05132.1"
                     /translation="MKSFNSALTAIRRSPYQSILSIIVLTLTFFIAFTFSIMTIGSQK
                     VLQYFETQPRVIAFFRLETENAEIDKLAEELRAKDYVKDINIVDKAEALEIYRQENQD
                     NPLLLELVTEDILPASIEVSAYNAENLEEISQILKNNQFVEDVDFQQDVMQSLIRWTN
                     ALRLFGLFFLGILLIVSFLMIMVTISLKVSAKRNSIKVMKFIGASNQFISRPYLLEGI
                     ILSIIASILAFAFYLAMLLYVTPWLSDFLQGIIEFPVSWQFLLYQFLLGLIAAVLLGT
                     FASLTAVKRMLKK"
     gene            complement(3738..4571)
                     /locus_tag="PLJ02_01550"
     CDS             complement(3738..4571)
                     /locus_tag="PLJ02_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOI05133.1"
                     /translation="MIIFSDVSKSFGDQETAIKHFSLEIKDGDFLVITGPSGTGKTTL
                     MKLLIKEYEPTSGEIEVDGEKYSQLKKSRIPELRRKIGVVFQDYKLIKEMNVWENIAL
                     PIYIQGKKDREVEERVTDLLNLVNLADKALFFPRQLSGGEAQRVSIARALACGPKIIF
                     ADEPTGNLDTQATLAITRLLKKINELGTTVLFATHDELVLKELAKEKIVKLEKKTAQP
                     EETNEEEAEQVKEIAHFEIADIKDEEEAEQDKEENKEKEESKEKKKKDIKIRKSKKDK
                     K"
     gene            complement(4604..5185)
                     /locus_tag="PLJ02_01555"
     CDS             complement(4604..5185)
                     /locus_tag="PLJ02_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05134.1"
                     /translation="MIEVRKIDIDENPKITDNLNPPNIIIENLDAEPKNQSHQQDKQP
                     MNKAQKKKFIIFAVIAVLAGTLTGYGTSRLSKRNDLTKGNVAQVAGEVNNIKPGDIFG
                     ISDRDTFSDSAQGYLEKGGVDGEGSHHLLREGGQSQTVALTSSVTDLDDFVGMEVKVW
                     GETNKAQQSGWFMDVGQVEVISIDAEPPSQTLD"
     gene            complement(5220..6308)
                     /gene="prfB"
                     /locus_tag="PLJ02_01560"
     CDS             complement(5220..6308)
                     /gene="prfB"
                     /locus_tag="PLJ02_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOI05135.1"
                     /translation="MTEDQLKQLVVDAQKFLASVDLVKEQEIIKQVQKASSEADFWQK
                     EEAQSEIKKQQRAQKNLDEYQLITEKLNDLQTLIELAKEENNQEANSELTTEIDKTSN
                     QLKKILKELELKHYLNKKFDHCGAILSIHPGQGGTEAMDWAEMLERMYQRYFERKNWP
                     FRIVNKIRGEDAGIKEVVFEIEADYAYGYLKGEHGTHRLVRQSPFNADNLRQTSFALV
                     EVLPIIEDNQEIDIKDKDLIWNFTRAGGAGGQSVNKLNTAVELTHTPSGITVKCREER
                     SQIQNKERALKILKAKLAKIQEEKMEDTLAKEKGSHQHASWGTQIRNYVLHPYQLVKD
                     TRTQVETSQTNQVLDGDLDQFILAFIRR"
     gene            complement(6313..7776)
                     /locus_tag="PLJ02_01565"
     CDS             complement(6313..7776)
                     /locus_tag="PLJ02_01565"
                     /inference="COORDINATES: protein motif:HMM:NF024594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HOI05136.1"
                     /translation="MAKKIIFTLLGIAVVLTLITVWPIMALQRNVKSYLSLNLNHSED
                     QQLITNTAEKIIKNYEKINKNLKKIFLADFAEDLSIILPHFLTEKNLTYLVILQNSDE
                     LRATGGFMGSYFFLEFSHGVWNLTEVEDIYTMAGQQTQFPNSPQGHDLYLSEGKGLSI
                     QDANWWPEVAISAQHILELWQEIAEQSPYVDQERDIGGIVFVNLNFIENLLEQIGPIQ
                     LTDYDQVVDAQNFAQLARADRLDFFPGSREKANFLNHSKVALANRLSQLSSQEYLALM
                     KVVLENLENKNIQLYSQEQNLEQIWQKHRFAGDMIRNNLDSFYFYSVESNVGINKANR
                     LVDREFHFYQNDGQIEQIQIKFTNHNQKPATINTNPNLKTANHLSYINYQRLYFAPDT
                     KIKQVMSIDQEEQKTPLAFSTQPYFNQNNQEFLELSLLLVVPEQSQVSLIIELESDNN
                     YQNLEIQKQAGVKQIPIYIYQNQQLVDSWTLTRDQLE"
BASE COUNT         2251 a         1439 c         1402 g         2807 t
ORIGIN      
        1 caataattgg attatcaaaa ttataaagag tgcggtgcac cgagatataa gtttgtaaaa
       61 atttttccat gtcactaacg ctattgccag gaataatttt gatccgattg taaagaacat
      121 tagtcattaa atttaataga gcattttctt taaaattggg aaagagcaac tcttcaattt
      181 cacaagcagc cacttcaata atccagttat aaaaattaat tcttttctta acttgaatgt
      241 cgtcgttact tcttggcaaa cgactgtcgg tcataatttt aatgtatttg ttaagaatgt
      301 tttctaattc ttcaacctgt ttacgaccaa ttcgaccgcg atcaaagtaa ccactacgca
      361 ttaactccaa ggccattggt tcagcaattt ccttggcttt gacatcgggc ataatgctga
      421 tgccatatag tttagacact aagcgtcgtt ttaaaatgcg ctcgatagca gcgcgacgca
      481 acaaattttc ttctttccaa tcaactactt ctctaacttt ttcgtaagct ttggcaattt
      541 ttgaagcaac ttcgtcgact tcaagtgccg cgatcactgg ttgatgacga tattcccaat
      601 ccatttggta ttgcttgata tagtcatcaa aataatgaga taaagttttt agttgtttcg
      661 tttcctttgt ttcttctgac ataaacgcta gtgttcatta tagcaaaaaa ctcgactttc
      721 gatataatgg cttcattatg gccgacaaat atcaagcaac ctgggtatct catagttcga
      781 ttagtgattt tttgcaatgt ccccgagctt attacttaaa aaatgtttat aaagatccca
      841 aaactaaaca caaaatccag ttgatttcac caccactggc tttgggggcg gctgtccaca
      901 gtgtaattga agcactgtca gtcattcccg ccgaacaacg ctttgcagaa agtttaatca
      961 aaaaatttga tcaagaatgg caaaagttta ctgggatcaa aggaggtttt tttgatgagg
     1021 caaccgaaat ggcttacaag gagcgtgggg ccgagatgtt gagaagggtc atgaataatc
     1081 ctggaccctt gactgattta gcggtcaaaa ttaaggaaga tttaccctgg ttttggctat
     1141 ctgaggaaga agagattatt ttgtgtggca agatagattg gttggagtat ttacccgaaa
     1201 cagaccaagt ccacatcatt gactttaaaa ctggtaaaaa cgatgaagat gagaattcac
     1261 tgcaattgcc catttattat ttattagtcc attattgcca gcatcgagca gtagcgaagg
     1321 ccagttattg gtatttggaa tcaagtgatg aaccagttga acaagaatta ccagatttag
     1381 cctgggctga agcagaggtg ttagccatcg ctaaaaaaat taaaacagtg cgtaaattag
     1441 aacactacaa atgtccgcag ggtgaaaatg gctgttgggc ttgtcggcca atggagcaaa
     1501 ttctcaaagg agaagcgcaa ttggtggggg aaaatgatta tcaacaagat gtttatgttt
     1561 tgcccagtaa gcagaaaaaa attgccagaa aagataacag ccggttgatt tagtttttga
     1621 ctagactggc aatttgttga atttgtgata aagaatagaa atcactaaaa tttttgtcat
     1681 aaccctccca tgggtcatgc ataatgtatt gatcacctct tttctcttta ataacaataa
     1741 aatgtgatcc atatgggcct gctgataatt ttacaatcac tggattttca tttaatttac
     1801 tatctaaagt gctttgagaa taagtattat attgatagta ataaccagca ggagtgggcc
     1861 aaggtcttga catgtaagca gtattggaaa agaaataact gctattatta gatatagtaa
     1921 taggattaac attctctcca aattttttct ttatcatagc tgtactactg atcaaacagc
     1981 ctacttccat gatactcatg ttgctacttc ctattaattg gttagcccaa cgttgatcgc
     2041 gttgactaaa aaaccatccg tctgggctat tttgttctgg caatacagat ccacttttgc
     2101 tactggaaaa agcttttaaa gaatttaatt cagctatcgc ttctgctaat ttttgttgat
     2161 aaatagtttc actgcttttt gtttcagcca gcaacttatt ttttacactt ttttgtgcat
     2221 ctaattcggc tttttgtctt tctaactcac ttttttttag gtttacctct gcttgttttt
     2281 cttcctttaa ttgtttttgt tgatcataag tagctcgttg taattcagtc cgatgcatga
     2341 tttctaaagt ttgttcctga gcttgattga tgtagcgata ttgagtaata aagtcattaa
     2401 aagaatcact ggcaaaaaag ttgaccttat attgtttacg actttgttta tagctagccc
     2461 tgacgcgttc aataagaatc tcactgagtt tattgaggga aatactcaag ccctcaatgc
     2521 gttgagttaa ttcattaatt tctttttcca attgataaat ttcattagtt gtttgttgaa
     2581 ttttgattga ttgtagttga atttggccat tgagaatatt aatggcagat tgaagagtat
     2641 tagcttcgct tttggcttgg ttaactttac tttcccagca tcttctttga tcattaaggc
     2701 aattactata ttgatcggta tcagtttctt ttagctcatc acacttaaac tcctgatcac
     2761 aatcttgagc aagggcggga ttggttttaa aacttaaaaa agaaaaagat atgagtagaa
     2821 aaactaatag gtgatagctg aacgttttaa ctttcatctc aattattttt tcaacatgcg
     2881 tttgaccgca gttaagctag caaaagtacc tagtagcacc gcagctatca aaccaagtaa
     2941 aaattgatag agtaaaaatt gccagctaac ggggaattca ataatgcctt gtaaaaaatc
     3001 actaagccag ggtgtgacat aaagtagcat ggctaaataa aaagcaaaag ctaggatgct
     3061 agcaataata gataagatga taccttccaa gaggtagggt cgactaataa attgattgct
     3121 ggctccaata aatttcatca ctttaatact attacgctta gcactgactt taaggctgat
     3181 agttaccata atcatcaaaa aggaaacgat taataaaata cctaaaaaga acaggccaaa
     3241 taaacgtaaa gcattggtcc agcgaattaa gctttgcatc acatcttgtt gaaagtcaac
     3301 atcttccaca aattgattgt tttttaatat ttgactaatt tcttctaagt tttccgcgtt
     3361 ataagcggaa acctcaatac tagcaggcaa gatgtcttca gtgaccaatt ccagcaaaag
     3421 tgggttatct tgattttcct gacgatagat ttctaatgcc tcagctttgt cgacaatatt
     3481 aatgtctttg acataatctt tggctcttaa ttcttcggct aatttatcaa tttcggcgtt
     3541 ttctgtttcc aatctaaaaa aggcaataac tcgaggttga gtttcaaagt attgcaaaac
     3601 tttttgactg ccaatagtca tgatggaaaa agtaaaagca ataaaaaaag tgagagttaa
     3661 gacaataata ctgagaatgc tctgataagg tgagcggcgg atagcggtga gggcggagtt
     3721 aaaacttttc ataagtatta ttttttgtct tttttacttt ttctaatctt aatatctttt
     3781 ttcttttttt ctttgctttc ttctttttcc ttattctctt ctttgtcttg ttcagcctct
     3841 tcttcgtctt ttatatccgc aatttcaaag tgggcaattt ctttgacttg ttcagcctct
     3901 tcttcgttag tttcttctgg ttgtgccgtc tttttttcta atttaacaat tttttcttta
     3961 gccaactctt tcagtaccaa ttcatcgtga gtggcaaaaa gtaccgtggt acccagttca
     4021 ttaatttttt tcaacaaacg agtaatggct aaagtagctt gggtatccaa attgccagtt
     4081 ggttcatcgg caaaaattat ttttgggcca caagccaggg ctcgagcgat gctgacccgt
     4141 tgcgcttctc ctccagaaag ttgtcgagga aagaagaggg ctttgtctgc caggttaact
     4201 agatttaata ggtcggtcac tctttcttct acttctcgat cttttttacc ctgaatatag
     4261 attggtagag caatgttttc ccaaacattc atttctttaa ttaatttgta gtcttggaaa
     4321 accactccaa tttttcttcg cagttctggg attcttgatt tttttagctg actatatttt
     4381 tcaccgtcaa cttcgatttc tccgctcgtt ggttcgtatt ctttgatcaa caatttcatc
     4441 agagttgttt taccagtgcc agatggtcca gtgattacta aaaaatcacc atctttaatt
     4501 tccaaggaaa agtgtttgat ggcagtctct tgatcgccaa aagacttact gacatctgaa
     4561 aagataatca taggcaactt ccattaaatc atcaactgag tgtttagtct agggtttggc
     4621 tgggtggctc ggcatcaatg gaaattactt ccacttgccc tacatccata aaccaaccag
     4681 attgttgagc tttattagtt tcaccccaca ctttaacttc catacccaca aaatcatcga
     4741 gatcggtaac tgaagaagta agggcaacag tttgactttg accgccttct ctcagcaagt
     4801 gatgtgaacc ctcaccatca acgccacctt tttctaaata accttgtgcg ctatcggaaa
     4861 aagtgtctct atcactgatg ccaaaaatgt cgccaggctt gatgttattg acctcaccag
     4921 ctacttgggc aacattacct ttagtcaaat cgtttctttt gctcaatctt gaggtgccat
     4981 agccagtgag agtgccagct aagacggcaa ttacagcaaa aataataaat ttcttttttt
     5041 gcgctttgtt catgggttgt ttatcctgtt gatgactttg attttttggt tcagcatcta
     5101 aattttcaat aataatgtta ggcgggttta gattgtctgt aatttttgga ttttcgtcaa
     5161 tatctatttt tctcacttcg atcatatgag ttttccttta ttttttattg taaagcaatt
     5221 tatcgacgaa taaaagctaa gataaattgg tctaagtctc catctagaac ctgattggtt
     5281 tggctagttt ccacttgtgt acgagtgtct ttgactaatt gataaggatg caaaacgtaa
     5341 ttgcgaattt gggtgcccca actagcgtgt tgatgactac ctttttcttt agctagcgta
     5401 tcttccattt tttcttcttg aattttggcg agcttggctt ttaaaatttt caatgctcgt
     5461 tctttatttt gaatctggct acgttcttct cgacatttca cagtaatgcc acttggtgtg
     5521 tgagtcaatt ccacggctgt gttgagttta ttaactgatt gtcctccagc tccacctgcc
     5581 ctagtaaagt tccaaattaa atctttatct ttgatgtcaa tttcctgatt atcctcaata
     5641 attggtaaaa cttctaccag agcaaaagag gtttgtctta agttatcggc attaaaagga
     5701 ctttgtctca ctagtcggtg ggtgccatgt tcgcccttta agtaaccata ggcataatca
     5761 gcttcaattt caaaaactac ttctttaatg ccggcatctt ctccgcgaat tttgtttaca
     5821 atgcggaatg gccaattttt tcgttcaaaa tagcgttgat acatgcgctc tagcatttcc
     5881 gcccaatcca ttgcctcagt ccctccttga ccaggatgaa tcgagaggat ggctccacag
     5941 tgatcgaatt ttttgttgag ataatgtttg agttctagtt cttttagaat tttttttagt
     6001 tgattgctag ttttatctat ttcagttgtt aattcactat ttgcttcttg attattttcc
     6061 tcttttgcta attcaatcag agtctgtaaa tcgtttaatt tttcggtgat taattggtat
     6121 tcatcaagat ttttttgagc tctttgttgt tttttaattt cactttgcgc ttcttctttt
     6181 tgccaaaaat ctgcttcact gctggctttt tgcacttgtt taataatctc ttgctctttg
     6241 actaaatcca cactcgctaa gaatttttga gcatccacca ctagttgttt taactgatct
     6301 tcggtcatga tcttattcta actgatctct ggttagagtc cagctgtcaa ctaattgctg
     6361 attttgataa atatagatcg gaatttgttt cacccccgct tgtttttgga tttctaaatt
     6421 ttggtaattg ttgtcgcttt ccagctcaat tatgagacta acttgacttt gttcaggaac
     6481 taccagcagt aaactcaatt ctaagaattc ttgattgttc tgattaaaat atggttgagt
     6541 agaaaatgct aatggtgttt tttgttcctc ttggtcaatt gacatgacct gtttaatttt
     6601 tgtatctggg gcaaaatata gtcgttgata attaatgtag cttaagtgat tagctgtttt
     6661 taagttggga ttggtattaa tggttgctgg tttttggttg tgattagtaa atttaatttg
     6721 gatttgttca atttggccat cgttctggta aaaatgaaac tccctgtcta ctaaacgatt
     6781 ggctttgttg atgccaacat tactttcaac actgtaaaaa taaaaactat ctagattgtt
     6841 tctaatcatg tctccagcaa atcgatgctt ttgccaaatt tgttcaagat tttgctcttg
     6901 actgtagagt tgaatatttt tattttctaa attttctaag accactttca ttaacgccag
     6961 atattcttga gaacttaatt ggcttaggcg atttgctaaa gcgactttac tgtgattgag
     7021 aaaatttgct ttttcacgag agcctggaaa aaaatctaag cgatcggctc tggctaattg
     7081 tgcaaaattt tgcgcatcaa cgacctgatc ataatctgta agctgaattg gtccaatttg
     7141 ctctaataaa ttttcaataa aattaaggtt gacaaaaaca ataccaccaa tgtcccgttc
     7201 ttgatcaaca taggggcttt gttcagcaat ctcctgccat agctcaagaa tgtgttgcgc
     7261 actaatagct acttctggcc accagtttgc atcttgaatc gacaatcctt ttccttcact
     7321 caagtacaga tcatgaccct gaggactgtt gggaaactga gtttgttgac cagccattgt
     7381 gtaaatatct tcaacttcag ttaaattcca cactccatgt gaaaattcta aaaagaaata
     7441 agaacccata aatccacccg tggctcttaa ttcatcacta ttttgtaaaa ttaccaaata
     7501 agtcagattt ttttcagtta aaaaatgcgg taaaattatg gataaatctt cagcaaaatc
     7561 agctaaaaat atttttttta aatttttatt aattttttcg taatttttta taattttttc
     7621 ggcagtattt gtgattaatt gttgatcttc actatgattg agattgagac tcaaataact
     7681 tttcacgttt ctttgtaaag ccataattgg ccaaacagtt atgagcgtga gcactaccgc
     7741 gatacccagt agagtaaaaa ttatcttttt tgccataatt tttgataggt gtttgctatc
     7801 actggccaag tgaggctatt actgatcttt ttatccaatt ttttcgtctt tgcaggcttg
     7861 attattgcct gagctattgc tggagcattc ttagccagc
//
LOCUS       DAOSEW010000027         7508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_8495_length_7508_cov_4.061318, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000027 DAOSEW010000000
VERSION     DAOSEW010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 7508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7508
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8495_length_7508_cov_4.061318"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..874
                     /locus_tag="PLJ02_01570"
     CDS             23..874
                     /locus_tag="PLJ02_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05137.1"
                     /translation="MKKNLTQSKSWLFHGLQVFLFLGAILLLGATLVNAQEPAAKTDL
                     IWQKERLQEEISQARQAYSQNLEDYRNKERLYTIAYDQYASLKTLASLEDLVIKTKAV
                     ALSRDQVLIKYLELLRLNLYASEGVELSVKNQYLQLLEENIDALTTHSQALEAKHSQS
                     EVQASLDEFTAFTDLNKMSEQVLALLAISRLQRIYDLALPLKKDVDQFLGVAESSALS
                     ALLRASEETDKTLTQAKNNLDLLWGKAKKPNALEGIYRNLTKELNPVYINLSQSLAYL
                     DELMNFK"
     gene            878..1246
                     /locus_tag="PLJ02_01575"
     CDS             878..1246
                     /locus_tag="PLJ02_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05138.1"
                     /translation="MAKKKYQYNQDQTIFVDQAPLWQKASQDKIDLKKNLTGNKNLKP
                     FYFMMAGVFLLFAFYIIVVFLPKREAQVISEEPQEQEEMLEADPLEQRIQSLKDDLRA
                     ADPTRQTLAFPNINFKISFP"
     gene            1269..1823
                     /gene="frr"
                     /locus_tag="PLJ02_01580"
     CDS             1269..1823
                     /gene="frr"
                     /locus_tag="PLJ02_01580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOI05139.1"
                     /translation="MSIDLASYRPRFIKALDHLQKELDALRTGRASAQILDSVRVEAY
                     GSLMSLAEVASVAVPDPQLIVIKPWDKSLLPAIEKAIQLAQLNLNPIVDKDLIRVPVP
                     TLTTERRQEMVKILHQRAEEGRVMLRQIRADIRKDIEKQEGEAGISEDDLKANFTELD
                     DLVKEYMEKIEELVKSKEKDLLTI"
     gene            1826..2968
                     /locus_tag="PLJ02_01585"
     CDS             1826..2968
                     /locus_tag="PLJ02_01585"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HOI05140.1"
                     /translation="MYEIIVFILVLSFLVLIHEMGHYLLARKNKINIKEFGLGYPPRL
                     VKLFTYQGTVFSLNAIPFGGFVQLEGENGPEDHTDRGANKKNNQEWGPFFTKSISARL
                     QTLLAGVFSNFIFGIIAFSIIFTQTGIPHDLGEQARIYELRDDSPAASAGLKENTNIV
                     SFYSQLDQQWVTVNNIKEVQDYVDAHLGEEVIVKTTDTCELTDCPDSYQEFTVYLRKD
                     SERAESEGAMGVVFTSVVYEKNPWYLAIIKGSLYGFKEAMALGLLILQALGNLVVDLV
                     NGRSVAMDVAGPVGIVHQAQTYGFFDGGFLNILSFAALLSINLGVMNLLPIPALDGGR
                     AVFILLEKVITKEKIEKVANWANYLGFCILILLMIFVSVNDVRNLF"
     gene            2982..3257
                     /locus_tag="PLJ02_01590"
     CDS             2982..3257
                     /locus_tag="PLJ02_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05141.1"
                     /translation="MAKKRRKIDKIRAQKRLAEAAKNATAKNTAAQDRNSLKTAASPA
                     VKPIDGLNKTANLFNYDPQLISQDLKKTLLISLVILVVLALITLRYT"
     gene            3291..4481
                     /locus_tag="PLJ02_01595"
     CDS             3291..4481
                     /locus_tag="PLJ02_01595"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HOI05142.1"
                     /translation="MSKKNLVILLIFYLVSSVISFFSFSYFSKEKVVAVNEPATEPKE
                     SILGGLLDIDPNEPRDQACPLNGKYYTLTEKKAWETRRPLFVMIENSPDARPHSGLSR
                     ADVVFEAVAEGGVTRFGALYYCAAQNENVVIAPVRSARTYFVDWASGFNLPMYVHVGG
                     ANLSGPSNALGQIADYGWRGENDMDQFSIGYPTFIRNYSRIPDKEVDTEHTMQTDTEA
                     LWAVAADRGWTNITPERVVNRQTIPATPWQEGYQGWTFEDEVGEPGAVRKISYDFWTG
                     YNQFSVEWNYDVDLDGYIRTNGGEVQYDLNNDEPIAPKTVIVLLTTEKGPINELKHML
                     YTTIGKGKALIFKHGQEPIEANWSKPSREAELQFTDVRGKAVELARGQIWISVLATGS
                     EIVY"
     gene            4636..5235
                     /locus_tag="PLJ02_01600"
     CDS             4636..5235
                     /locus_tag="PLJ02_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOI05143.1"
                     /translation="MSCLNDLLISKVRVKVLELFYKNPDEMYYVREITRLVKEEINAV
                     RRELERMLDCGLLKSEQRGNRLYYYLNKRYLYHQELQRIVAKSTGLGKKIRRNRRKLG
                     TLSYVMFSGTFTNRQQAKPDELDILVVGDVVLPELEELIRAEQKELGREINYAVLSAD
                     EFEFRKTRRDPFVMEVLYGVRVMIIGDENEFCHRDLPIN"
     gene            5257..6651
                     /gene="secD"
                     /locus_tag="PLJ02_01605"
     CDS             5257..6651
                     /gene="secD"
                     /locus_tag="PLJ02_01605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOI05144.1"
                     /translation="MSKKSNTRAFFWLLLLTIIAAYIAFPTRLAVDTKIINKKIQFVI
                     NKPPIDFYFWGKHVYKDFELKKGLDIQGGMQVVLRAKMNEIANEDRETALESAREIIS
                     RRVDLYGISEPTIQVARLEDDYRLIVELPGVDDPQQALDLVGKTAELEFRLERELSDA
                     ELAIATQSANFLDMYFAPTGLTGQQLKRAQLQFDQQTAKPQIALEFDDSGRDLFAEIT
                     KNNINKMLAIFIDGLPVVTPTISTAILDGRAIMTGDFSVEEAKNLAIQLNAGALPVNI
                     EVLEQKNIGASLGDESVQKSIIAGVVGLALVILFMVLYYGWLGVISSLALIIYAILTI
                     AIYKIIGVVLTLPGIAGLILTIGMAVDANILIFERMKEEQRAGRNYAEALELGFGRAW
                     DTVKDANLASIITALVLINPLNFSFLNTSGLVKGFGITFLLGSLLSMFTGMTVSKILV
                     KQLMPLIKPKEFQS"
     gene            6648..>7508
                     /gene="secF"
                     /locus_tag="PLJ02_01610"
     CDS             6648..>7508
                     /gene="secF"
                     /locus_tag="PLJ02_01610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOI05145.1"
                     /translation="MNLMKHKKIYFLISAFFLIPGLISLILFGLKPAIDFTGGSLLEF
                     QFIDEVKAENYSIEELKQSLQELYPVESIQKTGDNSLILRAKEITNQEKELIVDSIVN
                     EFGEIRVLRFETVGPILGQELLKKTITAILLVAAVIAIYIWQQFHDLKFGISAVLAMF
                     HDSLILLGIFSLLGHFRAVEVDVLFVTAVLTTLSFSIHDTVVLYNRIRELKKKHRHLS
                     LEEIANAAVWKTLGRSINDSFTIIIMLLSLVLLGGESIRYFTLALLIGAITGTYSSSF
                     TAVPILLVWEE"
BASE COUNT         2348 a         1150 c         1589 g         2421 t
ORIGIN      
        1 aaatcagagc tgaatttata aaatgaaaaa aaatctaaca cagtcaaaat cttggctatt
       61 tcatggttta caagtgtttt tatttttggg tgccatttta ttgcttggcg caactttagt
      121 taatgctcaa gaacccgcag ctaaaacaga tcttatttgg caaaaggaaa gattacaaga
      181 agaaattagt caagctcgcc aagcatacag tcaaaatttg gaagattacc gtaataaaga
      241 aaggctgtac accattgctt atgatcaata tgcttcatta aaaactttgg ctagtttgga
      301 ggatttagta atcaaaacga aagcagtagc tctcagtcga gatcaagttt taatcaagta
      361 tttagaatta ttaaggttaa atctttatgc gagtgaagga gtagaactat cagtcaaaaa
      421 tcaatatctc caattattag aagaaaatat tgatgcttta acaacacata gtcaggcctt
      481 agaggctaaa catagtcaga gtgaggtgca agccagttta gatgaattta cagcatttac
      541 tgatctaaat aaaatgagtg agcaggtttt agccttgtta gcgatttcac gactacaacg
      601 catttatgat ttagctttac cacttaaaaa agatgttgat caatttttgg gagtggctga
      661 aagtagcgct ctgagtgctt tattaagagc ttcagaagaa accgacaaga ctctgactca
      721 ggccaaaaat aacctcgatc tgttgtgggg aaaagccaaa aaacccaatg cgttagaagg
      781 tatttatcgc aacttaacta aagaattgaa tccagtttat attaacttat ctcaatcttt
      841 ggcttatttg gacgaattaa tgaactttaa atagattatg gctaaaaaaa agtatcaata
      901 caaccaagac caaaccattt ttgttgatca agctcccttg tggcaaaaag cctctcaaga
      961 caaaattgat ctgaagaaaa atttgactgg taataaaaat ttaaaaccgt tttattttat
     1021 gatggctgga gtatttttgc tttttgcttt ctatattatt gtagttttct tgccaaaaag
     1081 agaggcacaa gtcataagtg aagaaccgca agaacaagaa gagatgcttg aagctgatcc
     1141 actagaacag cggattcaat ctttaaaaga tgatttgaga gctgcagatc cgacccggca
     1201 gaccctggct tttcccaata ttaatttcaa gatcagtttt ccttaaaatt tgttacaatt
     1261 tacttattat gtcaattgat ttagcaagct accgtcctcg atttattaag gctctcgacc
     1321 atttgcagaa ggagcttgat gctttgcgta caggcagagc ttcagcacag attttagatt
     1381 cggttagggt ggaagcctat ggcagtttaa tgtctttggc tgaagtagcg tctgtggcgg
     1441 taccagatcc tcagttgatt gtaatcaagc cttgggataa gagcttgttg ccggcgattg
     1501 aaaaagctat tcaattggct caactcaacc tgaatccgat tgttgataag gatttaattc
     1561 gggttccagt acctacctta actactgagc gtcgtcaaga aatggtaaaa attttgcatc
     1621 aacgcgctga ggaaggtaga gtaatgctga gacaaatcag ggccgacatt cgcaaagata
     1681 ttgaaaaaca agagggtgaa gctggaatta gtgaagatga tcttaaggct aattttaccg
     1741 agctcgatga tttagttaaa gaatatatgg aaaaaattga ggagttagtc aagagtaaag
     1801 aaaaggattt gttaactatc taattatgta cgaaattata gtgtttattt tagttttgtc
     1861 ttttttggtg ctgattcatg aaatgggaca ttatttattg gcaagaaaaa ataaaattaa
     1921 tatcaaggaa tttggtttag gctatccgcc acgactagta aagcttttta cctatcaagg
     1981 tacggttttt tcactcaatg ccattccttt tggtggtttt gttcaattag agggtgagaa
     2041 tggtcccgaa gaccatacag accgtggtgc aaataaaaaa aacaatcaag aatggggacc
     2101 tttttttact aaatcaattt ctgccagatt gcaaacttta ttagctggag ttttttccaa
     2161 ttttattttt ggtattatcg cctttagtat tatttttact caaactggta ttccccacga
     2221 tttgggagag caagccagaa tttatgaact cagagatgat tcaccagccg ctagcgctgg
     2281 cctgaaagaa aataccaata tcgtgagttt ttatagtcaa cttgaccagc aatgggtgac
     2341 cgtcaataat atcaaggaag ttcaggatta tgttgatgcc catttgggtg aagaagtaat
     2401 tgttaaaact accgacacat gtgaattaac tgactgccca gatagctatc aagaatttac
     2461 agtttatcta agaaaagatt cggagagagc agagtctgaa ggggctatgg gcgttgtttt
     2521 tactagtgtg gtgtatgaaa aaaatccgtg gtatttagct attattaaag gttcgcttta
     2581 tggtttcaaa gaagcgatgg cgctaggttt attaattttg caagctttag gtaatttagt
     2641 tgttgatcta gtcaatggtc gctcagtggc gatggacgta gccgggccag taggcattgt
     2701 ccatcaagct caaacttatg gcttttttga tggcggtttt ttgaatattt tgagttttgc
     2761 cgcattgtta tcgattaatt taggagtgat gaatttatta ccaataccgg ctttggatgg
     2821 aggtcgggcg gtttttattt tgttagaaaa agtgattact aaggagaaaa ttgaaaaagt
     2881 agcaaattgg gctaactatc ttggtttttg tattttaatt ttgttaatga tttttgtttc
     2941 ggtaaatgat gtaagaaacc tcttttaaaa aaataaagat tatggcaaaa aaaagacgta
     3001 aaattgataa aattcgggcg caaaaaaggt tggcggaagc tgctaaaaac gccactgcta
     3061 aaaacaccgc tgctcaggac agaaattctt taaaaacggc ggccagccca gcagtcaaac
     3121 cgatagatgg actaaataag acagccaatt tgtttaatta tgatcctcaa ttaattagtc
     3181 aagatttgaa aaaaacatta ttaattagtc tggtaatttt agtagttcta gctttaatta
     3241 ctttgcgtta tacttaggat tgtcaatttt tggggaaagg tagacaagat atgtctaaaa
     3301 aaaacttagt tattttattg attttttatt tagtttccag cgtgatttcg ttttttagtt
     3361 tttcctattt ctcaaaggaa aaggttgtag cggttaatga accagccaca gaaccaaaag
     3421 aatctatttt aggtggttta ttagatatcg atcctaacga gccgagagat caggcttgcc
     3481 cgcttaatgg taaatattac actttaactg aaaagaaagc ctgggaaacc agacgaccgt
     3541 tatttgtcat gattgagaat tctccagatg cccgacctca ttcgggatta agtcgagctg
     3601 acgttgtttt tgaagcagtg gctgaaggtg gagtaactcg ttttggggct ctttattatt
     3661 gtgccgccca aaatgaaaat gtagtgattg ctccagttcg tagtgctcgt acttactttg
     3721 ttgattgggc ttcaggattt aatttgccaa tgtatgttca tgttggtgga gctaatttgt
     3781 ctggtcctag caatgcttta ggccaaattg ctgattatgg ttggcgtggt gaaaatgata
     3841 tggatcaatt ttccattggc tatccaactt ttattcgtaa ctatagtcgt attcctgata
     3901 aagaagttga tacagaacat accatgcaaa cggatactga ggcgctttgg gcagtggccg
     3961 ctgatagggg ttggactaat ataactccag agcgagtggt taatcgtcaa acaatccccg
     4021 ctaccccttg gcaagaaggt tatcagggtt ggacttttga ggatgaggtt ggagaaccag
     4081 gagcagtcag aaaaattagt tatgattttt ggactggcta taatcaattt tccgtcgaat
     4141 ggaattatga tgtagacctg gatggctata taagaactaa tggcggtgag gttcagtatg
     4201 acctgaataa tgatgagcca atagctccca agacagtgat tgtgctctta actactgaaa
     4261 aaggaccaat taatgagtta aaacacatgc tttataccac tattggtaag ggtaaagctt
     4321 taattttcaa acatggtcaa gaaccaatcg aagctaattg gagcaaaccc agtcgggaag
     4381 cggagcttca atttacggat gttagaggca aagctgtgga attggctcgt ggtcaaattt
     4441 ggattagtgt tttagctact ggcagtgaaa ttgtttatta attaatttga taaagtttcg
     4501 tcattaataa gccggctttc gccggctttt ttgattaaat tgttacaaat tcgttgttga
     4561 ttttttaatt tgaatcacgt aaaattgagt aaatttttgt ttcaaataat ttagcaaaac
     4621 aataaaggaa actttatgtc ctgtctcaat gatttattaa tttccaaagt tagagtcaaa
     4681 gtgctggaat tattttataa aaatccagat gaaatgtatt atgttaggga aattaccaga
     4741 ttagttaaag aagaaattaa tgctgttaga agagaactgg aaagaatgtt ggattgtggc
     4801 ttgttaaaaa gtgaacaacg cggcaatcgt ctctattatt atttaaacaa acgctacctt
     4861 tatcatcaag aattacaaag aattgttgcc aaaagtactg gtttgggtaa gaaaatcagg
     4921 cgaaatcgcc gtaaactagg tacactgtcg tacgtgatgt tttctggtac ttttactaac
     4981 cgtcaacaag ccaaaccaga tgaattagat attttagttg tcggcgacgt ggttttacct
     5041 gagctagaag aattaattag agctgaacaa aaagaattgg gtagagaaat taattatgct
     5101 gtgctaagtg ccgatgagtt tgaatttaga aaaacacgcc gagatccatt tgttatggaa
     5161 gtattatatg gtgtaagagt aatgattatt ggtgatgaaa atgaattctg tcatcgagat
     5221 ttaccaatta attaataatt cagagagata gttttcatga gtaaaaaatc aaacactaga
     5281 gctttttttt ggctgttatt actaaccata attgccgcct atattgcttt tcccacccgg
     5341 ctggctgttg atactaaaat tattaacaaa aaaattcaat ttgtaattaa taaaccaccc
     5401 attgattttt atttttgggg caaacatgtt tataaagatt ttgagttaaa aaagggtttg
     5461 gatattcaag gagggatgca ggtggtgttg cgagccaaaa tgaacgaaat agctaatgaa
     5521 gatagagaga cggctctaga atcagctcga gaaatcatta gtcggcgagt tgatctttat
     5581 ggaattagcg aaccgactat tcaagttgct cgtttagaag atgactatcg cctcattgtc
     5641 gaactgccag gggttgatga cccccagcaa gcgcttgacc tagtgggcaa aacggctgaa
     5701 ttagaatttc gattagaacg tgagttaagt gatgcggaac tggcgatcgc tacccaatct
     5761 gccaattttt tagatatgta ttttgcgccg actggtttaa ctggtcagca attaaaaagg
     5821 gcgcaattac aatttgatca gcaaacagct aaaccgcaaa tcgctttaga atttgacgac
     5881 agtggtcgag atctttttgc cgaaattacc aaaaataata ttaataaaat gttggccatt
     5941 ttcattgatg gtttgccagt agtgacaccg accatttcaa ctgccatttt agatggtcga
     6001 gcaattatga caggtgattt ttcggttgag gaagccaaaa acttagcgat tcagctcaat
     6061 gcaggtgcgc tacccgtgaa tattgaggtg ctcgaacaga aaaatattgg agctagtttg
     6121 ggcgatgaat cagtgcaaaa aagcattatt gctggagtgg ttggtttagc tttggttatt
     6181 ttgtttatgg ttctatatta tggttggtta ggagtaattt cttctttagc tttgattatt
     6241 tatgcgatct taaccattgc tatttataaa atcattggtg tggttttaac tttgccagga
     6301 attgctggtt taattttaac tatcggcatg gcagtagatg cgaatatttt aatctttgaa
     6361 agaatgaaag aagaacagcg agcaggcaga aattatgcag aagctctgga acttgggttt
     6421 ggtcgagctt gggatacagt taaagatgca aatctagctt cgatcattac ggctctagtc
     6481 ctcattaatc cgttaaattt ttcattttta aatacgagtg gtttggtcaa aggtttcggc
     6541 attacctttt tgttaggtag tttgcttagt atgtttactg gcatgactgt ttctaaaatt
     6601 ttagttaaac aactcatgcc actcattaag cctaaggaat ttcagtcatg aatttaatga
     6661 aacataaaaa aatttatttt ttaatttcag catttttttt gattcctgga ttaatttctt
     6721 taatcctttt tggactgaaa ccagcgattg attttactgg tggcagttta ctagaatttc
     6781 aatttataga tgaagttaaa gctgaaaatt attcaattga agagttaaaa caaagtttac
     6841 aagaacttta tccagtggaa agtatccaaa aaactggtga caatagtcta attttgcgag
     6901 ctaaggaaat tactaatcag gaaaaagagt taattgttga ttcaatagta aatgaatttg
     6961 gcgagattag ggttttgcgt tttgaaacag ttggtcccat cttaggtcaa gaattgttaa
     7021 aaaaaactat cactgcgatc ttattggtgg cagcggtgat tgctatctat atctggcagc
     7081 aatttcatga ccttaaattc ggcatttcgg cagtactggc aatgtttcac gattcattga
     7141 ttttattagg tatttttagc ttgctcggtc atttccgagc tgtggaggta gatgtcctgt
     7201 ttgtgaccgc tgttttgacc actctgtcct tttctattca tgatacagtg gttctctata
     7261 atcgcattcg agaattaaag aaaaaacatc gccatctatc tctagaagaa attgccaatg
     7321 cagctgtgtg gaaaaccttg ggcagatcga ttaatgattc ctttactatt attatcatgc
     7381 tgttatcgtt ggtattgttg ggtggagaaa gtatccgtta ctttactttg gctttattga
     7441 tcggagccat tacgggtacg tattcaagta gttttacggc tgtgccaatt ttgttggttt
     7501 gggaagaa
//
LOCUS       DAOSEW010000028         7384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_8699_length_7384_cov_4.640469, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000028 DAOSEW010000000
VERSION     DAOSEW010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 7384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7384
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8699_length_7384_cov_4.640469"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="PLJ02_01615"
     CDS             <1..783
                     /locus_tag="PLJ02_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05146.1"
                     /translation="VFPNPDTGSEWIEIYYVGENPVLASDYINFSISDDKRIIYTFQG
                     DEVWSDNFLVVAVAGLNNDQDSVILKNAEELVIDQMSYTLSQKNLSWLRINEQESNFI
                     LGEASPLQFNNIPPSATPSPNPTLSPLPSPSLTSPSSHPNPDVINTPTPTASPIDLAQ
                     NNNTNQVETKPDKKQNYHQLSPEELAQNYFNNYQDYHNLQITYSQEKQFPQTRLVFLG
                     QKMLKPAIIDAIIGSSLLVLAAILLSYDNKTKKEKERFSSLF"
     gene            734..1135
                     /locus_tag="PLJ02_01620"
     CDS             734..1135
                     /locus_tag="PLJ02_01620"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOI05147.1"
                     /translation="MITKPKRKKSAFLLYFRAYLPSILWASLIFFLSNQQTLPGFDLS
                     LLDFLFKKSAHIFVYAVLYYLLFWAYLKTNPSQTLNKKHYVIPLIIGLIYALSDELHQ
                     STVNGRHPKLRDVAYDFLGMMSILLHQQKLL"
     gene            1218..1493
                     /locus_tag="PLJ02_01625"
     CDS             1218..1493
                     /locus_tag="PLJ02_01625"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOI05148.1"
                     /translation="MTKSELINVVAKKAHLTKKAAGEAVEAVFDEVIRTLKKGDKAVI
                     SGFGTFYVGKVEDKHVEPFGDKTKRQLVKGHRVVNFRVGKPLKREIW"
     gene            complement(1576..2085)
                     /gene="rpsI"
                     /locus_tag="PLJ02_01630"
     CDS             complement(1576..2085)
                     /gene="rpsI"
                     /locus_tag="PLJ02_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOI05149.1"
                     /translation="MAIRMNKKTSKVKKVVKKTPKINAKMAQSKSAVDFKLPSNKYTE
                     ATGHRKIATARVRIYEGNGDLVVNQELIADYFSGIRNAAAIYMQPFNLTGTKGKFALS
                     ARVSGSGKRAQLDAVVHGLASALVKYNPEFRTFLKPAGLLTRDDRMKETRKVGRGGKA
                     RRKRQSPKR"
     gene            complement(2087..2515)
                     /gene="rplM"
                     /locus_tag="PLJ02_01635"
     CDS             complement(2087..2515)
                     /gene="rplM"
                     /locus_tag="PLJ02_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007020850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOI05150.1"
                     /translation="MPKNKTFIEKKEAITRQWYEYNAEGQVLGRLATEVAQKLIGKHK
                     VTYTPHNDNGDYVVVINAEKIEVTRNKASKKIYYRHTGFPGGLKQRTFNEMIEKNPSK
                     VIEQAVKNMLPKNRLRDQRLARLKIIVGDQHPYKNLIKAK"
     gene            complement(2526..2987)
                     /gene="rplQ"
                     /locus_tag="PLJ02_01640"
     CDS             complement(2526..2987)
                     /gene="rplQ"
                     /locus_tag="PLJ02_01640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOI05151.1"
                     /translation="MKHGVKAKHFNRDTQSRKALLRNSLRGLIEHGQIETSEARAKEI
                     RRLADKLINQAQKGDVATRRRIHRYFGKRDVVNTLFDQVVPAVAERKSGFCSLEKLAN
                     RRGDNMPVYRLSLLVEEKKWQSLKNDSNKTDKKASASKKTETKKETTKKKK"
     gene            complement(2997..3941)
                     /locus_tag="PLJ02_01645"
     CDS             complement(2997..3941)
                     /locus_tag="PLJ02_01645"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOI05152.1"
                     /translation="MTDNANNYSVMNPIFTVSEVESNGSRSTIVIEPLESGYGHTLGN
                     ALRRVLLTSLPGLAITKLSIDGISHQFSTYEGLKEDVVDLILNLKNIRIKSDSAETGV
                     LRLNVKGPKEITAKDIECEAGFEVINKQLYLATLTKGKNLNIEMIVEPGIGYKLASEI
                     ELENVGQIAVDALFSPVIKVAYHVEATRVGSRTDYDRIVFDIQTDGSITPLEAVKKAS
                     EILLAQFKQIIDPVLPEVKEELPAISPEELEVMRLTVEELDLPTRIANALRKGGFETV
                     KDLKAANKTEISKVKNLGGKSIDLIEEALSKKGVVLSA"
     gene            complement(4004..4612)
                     /gene="rpsD"
                     /locus_tag="PLJ02_01650"
     CDS             complement(4004..4612)
                     /gene="rpsD"
                     /locus_tag="PLJ02_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOI05153.1"
                     /translation="MARHSGPKQKLQRQIGEDLGLKTNALKTARRLAIRPGQHGVKTH
                     RKMSDYALQLREKQKLKYIYGILERQLGKIFEEASKNPAATGTVLLSLLERRLDNVIY
                     RAGFAVTRASARQFVGHGHYLVNGKKMNIPSYTVKVGDVITMKKNRTAKIPQVVVAME
                     EERPIPNWLEIKGTSIKVAKLPVREDIVEKIDEQLIVEYYNR"
     gene            complement(4630..5043)
                     /gene="rpsK"
                     /locus_tag="PLJ02_01655"
     CDS             complement(4630..5043)
                     /gene="rpsK"
                     /locus_tag="PLJ02_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOI05154.1"
                     /translation="MAVSSKKTTTKKKKSNYQRATKIVPKGRLYVSATFNNTIVSVTD
                     EKGNVLCWESTGGSGFSGSRKSTPYAATITVENAINKAKNNYGMNQMEVYLKGPGPGR
                     DVALRVLRAQGIGVALIADLTPVPHNGTRPRKARN"
     gene            complement(5055..5459)
                     /gene="rpsM"
                     /locus_tag="PLJ02_01660"
     CDS             complement(5055..5459)
                     /gene="rpsM"
                     /locus_tag="PLJ02_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOI05155.1"
                     /translation="MPRIAGHDIPDNKKINFSLRYIYGVGPKVAEEIIKKCKINPDKR
                     AKDLTGEEINRIQAALDAYLIEGDLRREINDNIARLKRIQSYRGMRHSAGLPSRGQRT
                     RSNARTRRGARRTIGSVTKEAASKLEETKKTN"
     gene            complement(5463..5576)
                     /gene="rpmJ"
                     /locus_tag="PLJ02_01665"
     CDS             complement(5463..5576)
                     /gene="rpmJ"
                     /locus_tag="PLJ02_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005987264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOI05156.1"
                     /translation="MKVKASLKKICKNCKLVKRHGKLYVICTNPKHKQRQG"
     gene            complement(5656..5991)
                     /locus_tag="PLJ02_01670"
     CDS             complement(5656..5991)
                     /locus_tag="PLJ02_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010939491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05157.1"
                     /translation="MGKVQEHKRAAKAKRQVDFTPSVASSQSDRFEIIGEVESCLPNT
                     MFRVRVIDSTAEQMKGRLLLGTLAGKMRLYRIRVMPGDRVKGYVSKYDLTKCKITYRT
                     TNGQSGTNQ"
     gene            complement(6041..6625)
                     /locus_tag="PLJ02_01675"
     CDS             complement(6041..6625)
                     /locus_tag="PLJ02_01675"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HOI05158.1"
                     /translation="MKLVLIGIQGAGKSTQGNLLSEKYNIPYLSSGHIFRQMAKSKTA
                     LGRFVKETINAGFLIPDDKTVEIVIEYLKKPEYQNGFILDGFPRTVPQAKAFEKFTKG
                     EEIVILIDVSDREALWRISGREADREDETLKAIRKRIDLFHEKTSKVLDYYSDRKHLA
                     VVDGEQSVQEVFNDIVLKVERIRNKDQKKGKSKK"
     gene            complement(6647..>7384)
                     /locus_tag="PLJ02_01680"
     CDS             complement(6647..>7384)
                     /locus_tag="PLJ02_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY family transport protein"
                     /protein_id="HOI05159.1"
                     /translation="GILSQIPASIGQSFVAGNDGQLTVILVMLVLTLAVIALVVFFNE
                     AVRKVNIQYAKRIQGGRQVGGQSSHLPIKVNVAGVMPIIFAVSLMMLPPFLGRILTLT
                     GREFWVNLGEQLNIIFSPTSIWYLLIYFVLVFVFSYFSAIIFFNAEDISEELKKSGAF
                     IPGIRPGGPTRKYLEYVVTRVTFAGAIFLASIAVLPTIAQMITGIQALAIGGTSLLIV
                     VSVILETARQVESQLLNENYDRYSQLS"
BASE COUNT         2180 a         1566 c         1176 g         2462 t
ORIGIN      
        1 gtctttccca atccagacac tggtagtgag tggatagaaa tttactatgt gggcgaaaat
       61 ccagtgttgg cgagtgacta cattaacttt agtattagtg atgataagcg gataatttat
      121 acctttcaag gtgatgaggt ttggtcggat aattttttag tagttgcagt tgctggcctt
      181 aataatgatc aggacagtgt cattctcaaa aatgctgaag agctggtgat cgaccaaatg
      241 agctacacac tcagtcaaaa aaatttaagt tggttaagaa taaatgagca agaatcaaac
      301 tttattttag gagaagctag ccccctccaa tttaacaata ttccgcctag cgcaaccccc
      361 agtccaaatc ctactctttc acccttgcca agcccttcac taactagtcc tagctcgcat
      421 cctaatccag atgtcattaa cacacccact cccacagcta gtcctataga tctggcacaa
      481 aataataata ctaaccaagt agaaactaaa cctgataaaa aacaaaatta tcaccaattg
      541 tccccagaag aattagctca aaattatttt aacaactacc aagattatca taacttacag
      601 atcacttact cacaagaaaa acaatttcct caaacacgcc tagtatttct tggtcaaaaa
      661 atgctcaaac cagcaattat cgatgctata ataggaagtt ctttactggt tctagcagca
      721 attttattaa gttatgataa caaaaccaaa aaggaaaaag agcgcttttc ttctttattt
      781 tagagcttat ttacccagca tactctgggc cagcctaatt tttttccttt ccaatcaaca
      841 gacactacca ggtttcgatt tatctctttt agattttctc tttaaaaaat ccgcccatat
      901 ctttgtctat gcagtacttt actacctact tttttgggct tatttaaaaa ctaatcctag
      961 ccagacactg aataagaagc attatgtaat ccccttaata atcggcctca tttatgcgct
     1021 gagtgacgaa ctacatcaat caacagttaa tggccgtcat ccgaagctgc gagatgtcgc
     1081 ttatgatttt ttaggaatga tgtctatatt gttacatcaa caaaaattac tataattgct
     1141 acttgactta ataagtcaaa ctagtacact aattcatacg taaaaggact atttaaaagt
     1201 taaaaaagga gaaaaccatg accaaatctg aattaatcaa cgtagtagcc aaaaaagccc
     1261 atcttactaa aaaagctgct ggtgaagcag tagaggcagt atttgatgag gtaattcgta
     1321 ccctaaaaaa gggtgataaa gccgttattt ctggctttgg tactttttat gtaggaaaag
     1381 tcgaagataa gcatgttgaa ccttttggcg ataaaaccaa acgccaatta gttaaaggtc
     1441 atcgcgtggt taatttccgc gtgggcaaac ctctcaaaag agaaatttgg taatttttct
     1501 aaaataaaat aattaaaaac ccaccgcttg gtgggttttt tgtatctata atcaattgct
     1561 aataaatgaa actgtttaac gcttaggtga ttgtcgtttg cgacgagctt taccaccacg
     1621 accaactttt ctagtttctt tcatccgatc gtcgcgagtg agtaaacctg ccggttttaa
     1681 aaaggtacga aactctggat tatatttaac caaggcactg gccaaaccat ggaccactgc
     1741 atcaagttgt gctctttttc ctgagccact cactcgggcc gataaggcaa atttaccttt
     1801 agtacctgtt aaattaaaag gttgcatata aatggctgca gcattacgaa taccagaaaa
     1861 ataatcagct attaactctt gattgacgac taaatcgcca ttaccttcat aaattcttac
     1921 tcgagcagta gcaattttcc gatgaccggt agcttcagta tatttattgc ttggtaattt
     1981 aaaatcgaca gccgatttac tttgagccat tttggcatta atttttggag tttttttaac
     2041 tactttttta accttgctgg tttttttatt catgcgaatt gccatattat tttgccttta
     2101 tcaaattttt gtaggggtgt tgatcaccaa cgatgatttt gagtcttgct aaacgttgat
     2161 cacgtaagcg attcttaggc agcatatttt tgactgcttg ttcaatcact ttactaggat
     2221 ttttttcaat catttcatta aaagttctct gtttaaggcc accggggaaa cccgtgtgtc
     2281 gatagtaaat ttttttgcta gccttgttac gggttacctc aattttttcc gcattaataa
     2341 ccactacata atcgccattg tcattatgcg gagtatatgt cactttgtgc ttaccaatga
     2401 gtttttgcgc tacttccgtc gctaaacgac ctaaaacttg gccttccgca ttgtattcat
     2461 accactgacg agtgattgct tctttttttt caataaaagt tttatttttt ggcatgtttc
     2521 tttacttatt ttttcttttt agtagtttct ttttttgtct cggttttttt gctggcactg
     2581 gcttttttgt cagttttgtt gctatcattt ttcaaactct gccacttttt ctcttctacc
     2641 aacaaactca atcgataaac cggcatatta tcaccacgtc gattagctaa cttttctaaa
     2701 ctacaaaaac cacttttgcg ctcagcgact gccggcacta cctgatcaaa caaagtatta
     2761 actacgtctc tcttaccaaa gtaacgatga atgcgccgtc gcgtagccac atctcccttt
     2821 tgagcctgat taattaattt atctgctaaa cgacgaattt ctttagctcg ggcttcacta
     2881 gtttcaattt ggccatgttc aattaaacca cgtaaagaat tacgcaataa agccttccgg
     2941 ctctgcgtat cacgattaaa atgtttagct ttaactccgt gtttcatatc tcattcctaa
     3001 gctgataaaa ccactccttt tttggatagg gcttcctcaa ttaaatcaat gcttttacca
     3061 ccaagatttt taaccttaga aatctcggtt ttattagcag ctttgagatc tttcactgtc
     3121 tcaaaaccac ctttacggag agcgttagca atccgtgttg gtagatccaa ttcttcaaca
     3181 gttaaacgca tcacttctag ttcctcagga ctgatcgctg gtaactcttc tttaacttct
     3241 ggtaaaaccg gatcaataat ttgtttaaat tgcgctaata aaatttcact agctttttta
     3301 acggcttcga gtggagtaat actaccatcg gtttgaatgt caaaaacaat ccgatcatag
     3361 tcagttcgac taccaactcg tgtcgcttca acatgatacg ctactttaat aactggggaa
     3421 aataacgcat caacagcaat ttgaccaaca ttctccaact caatttcact tgccaattta
     3481 taaccgattc caggttcaac gatcatttca atattcaaat tcttaccctt ggttaaagtg
     3541 gctagataga gttgtttatt aattacttca aaaccagctt cacactcgat gtctttggcc
     3601 gtaatttctt tagggccctt cacatttaaa cgtaagacac cagtttctgc actgtcggat
     3661 ttaatgcgaa tgttttttaa atttaaaatt agatccacta catcctcttt taaaccttca
     3721 taagtgctaa attgatgact aatgccatca atacttaatt tagtaatagc caatcctggc
     3781 aatgacgtca ataatactcg acgtagagca ttacccaaag tgtgaccata accactttcc
     3841 aatggctcaa tcacaattgt tgatctcgaa ccattgcttt ctacttcgct aacagtaaaa
     3901 attgggttca taacgctgta attatttgcg ttgtctgtca tattcatttt ctcctttgtt
     3961 tcgcctccct ctgctgagga atccgtttct agatttataa ttattaccga ttgtaatatt
     4021 caactattaa ttgctcgtca atcttctcga cgatatcttc tctaactggt aatttagcta
     4081 ccttaataga agtccctttg atttctaacc agttaggtat tggtctttct tcttccattg
     4141 ctacaactac ttgaggaatc ttagctgtcc tattcttttt catagtaatg acatcaccaa
     4201 ctttaacagt ataggatgga atgttcattt ttttaccatt cactaaatag tgaccatgtc
     4261 ccacaaattg tctagctgaa gcgcgagtca cagcaaaacc agcgcgataa ataacattgt
     4321 ctaaacgtct ttctaataaa ctcagtaaaa ctgttcctgt agcagctgga tttttgctag
     4381 cctcttcaaa aattttacct agttgtcttt ccaaaattcc ataaatgtat tttaattttt
     4441 gcttctctcg caattgtaaa gcataatcag acatttttcg gtgagtttta actccatgct
     4501 gacctggcct gattgctaaa cggcgagcag tcttgagagc attagtcttt aaacctaaat
     4561 cttcaccgat ttgtcgttgt agtttttgtt tgggaccact gtgtcttgcc atattcaatt
     4621 cctagaactt tagtttctag cctttcttgg tcgagtacca ttgtgtggta ctggagttaa
     4681 atcagcaatt aaagccaccc caatgccctg agctcgtaaa acacgaagcg ccacatctcg
     4741 accaggacca ggacccttta aataaacttc catttggttc atcccgtagt tatttttggc
     4801 tttattaatc gcattctcta ctgtaatggt agctgcgtaa ggagtagact tacgtgaacc
     4861 actaaaacca gaaccaccag tgctttccca acagagcacg ttgccttttt catcagtcac
     4921 actgactatg gtattattaa aagtagcgct gacatacaat ctgcctttag ggacaatttt
     4981 tgtagctctt tgataattag attttttctt tttagtagta gtttttttac tgctgactgc
     5041 cataaaaacc tttgttagtt agtctttttt gtttcttcta atttgctggc ggcttcttta
     5101 gtcactgaac cgatagttcg tctagctccg cgtctagttc tggcattgga tctggttctt
     5161 tggccacgag aaggtaaacc agcgctgtgc ctcattcccc gatagctttg aattcttttt
     5221 aagcgagcaa tgttgtcgtt gatttctcgg cgcaaatcac cttctattaa ataggcatcg
     5281 agggctgcct gaatacggtt gatttcctca ccagtcaaat ctttggctct tttgtctgga
     5341 ttaattttgc actttttgat tatttcttca gctacttttg gtccaacacc ataaatatag
     5401 cgtaaagaaa aattaatttt tttattatct ggtatgtcgt gtcctgcgat acgtggcata
     5461 gtttatcctt gcctctgctt gtgtttggga ttagtacaaa tgacgtacag tttcccatgc
     5521 cgcttaacaa gcttgcaatt tttacaaatt ttctttaacg atgctttaac tttcataatt
     5581 acagacgtgc aatataccct actggactaa atttttaaag agtgaaattt gtccttccag
     5641 gaatattgat ttcccctatt gatttgttcc tgattgccca ttagttgtgc gataggtaat
     5701 cttacatttt gttagatcgt atttactaac ataacctttg actcggtctc ctggcataac
     5761 ccgtatacga tacagacgca ttttgccagc caaagtccct aacaacaaac gacccttcat
     5821 ttgttcagca gtcgagtcaa ttactctaac tcgaaacatc gtgttaggta aacagctctc
     5881 aacttcgcca atgatttcaa aacgatcact ctgactacta gctacagatg gtgtaaaatc
     5941 aacctgtcgc ttagctttag ccgctcgttt gtgttcttgt acctttccca taaacgagca
     6001 ttataccaga tttagcaaaa aaaatcactt ataaatctga ctatttttta gatttaccct
     6061 ttttctgatc tttatttcta attctttcca cctttaagac gatatcgttg aaaacttctt
     6121 ggacactttg ttcaccatca acgactgcta aatgtttccg gtctgaataa taatccagta
     6181 ctttcgaagt tttttcatgg aaaagatcaa ttcttttgcg gatagctttg agagtttcat
     6241 cttctcgatc agcctctcta cctgaaattc tccatagcgc ttctctatct gatacatcaa
     6301 ttaaaatgac tatttcttca cccttagtaa atttttcaaa agctttagct tgaggtacag
     6361 tccggggaaa accatcgaga ataaaaccat tttggtattc tggttttttt agatattcga
     6421 tcacaatttc aacggtttta tcatctggaa ttaaaaaacc agcgttaatg gtttctttaa
     6481 caaaacgacc taaagccgtt ttgctcttgg ccatttgcct aaaaatatgt ccagaggaca
     6541 aataaggtat attatatttt tcagataata aattaccttg ggttgacttg cctgccccct
     6601 gaataccaat taaaactaac ttcataatcc ctttcacatt aataatttaa ctgagttggc
     6661 tataacggtc gtaattttca tttaataatt gtgattcaac ctgtctagca gtttccaaaa
     6721 taaccgaaac cacaattaat aagctagttc caccaatggc taaagcctga ataccagtaa
     6781 tcatttgggc aatagtaggt aacactgcaa tactggccaa aaagatggct ccggcaaaag
     6841 tcactctagt gaccacatat tccaaatatt tacgagtcgg acctcctggt ctaataccag
     6901 ggataaaagc gccacttttt ttcaactctt cactgatatc ttcagcatta aagaaaatga
     6961 tcgcactaaa ataactaaaa acaaaaacta aaacaaaata aattaacaga taccaaatac
     7021 ttgttggtga aaaaattata ttgagctgct cgcccaaatt aacccaaaat tctcgaccag
     7081 tgagagttag aattctcccc aaaaatggag gtaacatcat taacgacaca gcaaaaatga
     7141 taggcatcac ccctgccaca ttaaccttaa ttggtaaatg gctactttgg ccaccaactt
     7201 gacgaccacc ttgaatgcgt ttggcatatt ggatattaac tttacgtact gcttcattaa
     7261 aaaagacaac taaagcaatc actgctaaag ttaagactaa catcactaaa atcacagtga
     7321 gttgaccatc attgccagcg acaaaagatt gaccaatact ggccggaatt tgactaagaa
     7381 tacc
//
LOCUS       DAOSEW010000029         7133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_9153_length_7133_cov_4.064001, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000029 DAOSEW010000000
VERSION     DAOSEW010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 7133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7133
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9153_length_7133_cov_4.064001"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="PLJ02_01685"
     CDS             <1..610
                     /locus_tag="PLJ02_01685"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HOI05160.1"
                     /translation="PAQHPNPRPLLLAPIQGHNNKKWRRYCADALLEIGFDIYGFGGW
                     PLDHNGQFDYQLAQFNAQLTPDEYPRFALGVGSLENIVRLYLMGYQFFDCVLPTRDAR
                     HQRLYIFQTDPQALDLANIKKLATNHQLDKIFDYLYIGRSKYQTAFQQISEHCDRPIC
                     QNQQISRAYLYHLFKVKDSSAWRLATLHNLRTYQLLMEALSR"
     gene            702..1442
                     /gene="radC"
                     /locus_tag="PLJ02_01690"
     CDS             702..1442
                     /gene="radC"
                     /locus_tag="PLJ02_01690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOI05161.1"
                     /translation="MNKKSHQPDQSKSLLTCRPREKLYYSGKNSLNNAELLAIILNSG
                     HKNLPVLNLAKNILKKIPLNQWHEIFLLKNHQREQQLFKDLQDIPGLGFAQASKIIAA
                     IELGRRSIEQAKTSPLHSPQQVYHYCSELKNKKQEYCLAFFLNGQQELLLKKIITIGG
                     LNFNYLEGREIFEIAFQLQASNLILVHNHPSGNLQPSLNDLQVSKKLAELAEAMGINL
                     IDHLIVSSQGFLSIREKCGDLKKLDCQF"
     gene            complement(1473..3002)
                     /gene="cysS"
                     /locus_tag="PLJ02_01695"
     CDS             complement(1473..3002)
                     /gene="cysS"
                     /locus_tag="PLJ02_01695"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOI05162.1"
                     /translation="MKLYNTASKSIEEFHPLEDKSVKLYACGPTVYDYTHLGHLRKYV
                     LDDILVRTLRHAGYEVKHVQNITDVGHLTSDDDVGEDKLEKGAKKYHTGAEALARRFE
                     DYFFYSMDLLGVLRPSLSCRATDHIQAQLEMVLALEKKGLTYVIEGDGVYFDTSKIDD
                     YGKLINPDPKLAKQVLMAQREGERVDKVIGRKHPTDFALWKFERPGENRQMVWPSPWH
                     ERSFPGWHIECSAMALEHLGEQIDIHTGGVDHINIHHTNEIAQTEAVTGKKPFSRYWV
                     HHNYLFIDGQKMSKSLNNFYTIDDVIERGFAPRALRLLFLMSHYRSEQNFTWDSLAAA
                     QKAYLKLARFVLDLRNSKRNSELELSDFPGAVQLHHDFFDAIENDLKTNEALTVLWEL
                     MQSDLDSDVLYRLIMEFDQVLGLGLAGLTEESLLEAVKKALAKTIIDQDFAQKMGVQE
                     NLLQLSDLPDSIQELIKKRQLARRDQDFTEADRLRAELKEQGYELLDLADGQLAIRKI
                     K"
     gene            complement(3028..3687)
                     /locus_tag="PLJ02_01700"
     CDS             complement(3028..3687)
                     /locus_tag="PLJ02_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05163.1"
                     /translation="MEGGPSFGIGAKSQLTTSGGFRQTFGGSISEEHLDKAAAADSGH
                     NEQLAQQSSVLGQRRAVQEAAAIQGANKTALAQTPEKIATQPRAIAGLGQELLKRPVK
                     DFKDSFQAMFDINTLLNIQPKTPKEQAKQKRVAARWQGLTNEEQAEAKRIYEANLAKK
                     RQEEADKEKARQELEAQEAQELVVPKGRETGFRGFSGMGSKQKAQTLIQRQRTTLGGV
                     E"
     gene            complement(3694..4113)
                     /locus_tag="PLJ02_01705"
     CDS             complement(3694..4113)
                     /locus_tag="PLJ02_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05164.1"
                     /translation="MTDNQDPLQALKTLIDNKENVSVATSATSGELTPEQQAAQQQAN
                     AEEEAKLQAQLKAHEQQQAVLDQQALENLRTELPQVVSTGSSKENTQDVTQDRVTQDS
                     TQDGVTQNSAQDSTQGPANEQATQQLIHEVEQLKRLD"
     gene            complement(4206..4511)
                     /gene="raiA"
                     /locus_tag="PLJ02_01710"
     CDS             complement(4206..4511)
                     /gene="raiA"
                     /locus_tag="PLJ02_01710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOI05165.1"
                     /translation="MKVIVQAKSLKVTRALREFIERHSQKLEKLGQEITAVRVYLEQK
                     TKKSSKSKNVLVKYKIEVPGQDLWIEVAGYDFYDAIVDAVDAATRKLRRSKEKQLEH"
     gene            4698..5336
                     /locus_tag="PLJ02_01715"
     CDS             4698..5336
                     /locus_tag="PLJ02_01715"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOI05166.1"
                     /translation="MTENLDANTNLVDKLNKANNNEPNNITQEHQDKSAPKEQHQQLS
                     NWGKVRRFVSAALIGISLASCRQTLTNEEQRNLDTLGDNSPMVATAYNIYLNNEAQNN
                     QTNTPEAVQAFNEPELFEVPAVDQTIFPGSRVQVFETDGYGLNIRSEAGINSAVVTNA
                     AEGTIFKVIELVGEKDGYQWALVKSEPKEGQNEIVGYAAVDWLQVLREAPIK"
     gene            complement(5364..6005)
                     /gene="recR"
                     /locus_tag="PLJ02_01720"
     CDS             complement(5364..6005)
                     /gene="recR"
                     /locus_tag="PLJ02_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013171006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOI05167.1"
                     /translation="MQKLPKAVRRLIEKFEALPGIGPKSAQRLVFYLLHTPESQLVDF
                     ADRLLALKRETVMCSVCHNVAEQDPCDICQDKQRDQDLLCVVEQPLDLLAIERSGKYL
                     GHYHVLHGSIAPLNNIGPDQLYLRDVSKRLRDSSGKLIVKELILATNPTMEGEATALY
                     IAEMLKNDDFFAKKIESGALTITRIGRGLPIGADIEYADGVTLARALEGRRHL"
     gene            complement(6023..6736)
                     /gene="tmk"
                     /locus_tag="PLJ02_01725"
     CDS             complement(6023..6736)
                     /gene="tmk"
                     /locus_tag="PLJ02_01725"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOI05168.1"
                     /translation="MQSNGKRGLLITLEGGEGGGKTTQIVRLKNHLIEAGKDVVVLRE
                     PGGTVISEQIREVVLSSKNTGIAFTTEVLLFQAARSQIYREIVLPSLKAGKVVIMDRS
                     RDSSVVYQGMVRGFGTELIEQLNDISTKKTYPDLTFLLDIPVNIGLKRRNGTGEVNRL
                     DMEATDFHEKVRQGYLTLAKKDQEEHGKKARWVIIDARPSIDQVEDNIWQVVKQYLAK
                     KELSTWEDNLAGVDLEYSK"
     gene            complement(6847..>7133)
                     /locus_tag="PLJ02_01730"
     CDS             complement(6847..>7133)
                     /locus_tag="PLJ02_01730"
                     /inference="COORDINATES: protein motif:HMM:NF012895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOI05169.1"
                     /translation="VPADSFVLVAARSSLHQRGLMLINGIGIGDSDYCGSDDEYQAAV
                     YNFSQESVLIKKGERVAQMMVLRREKVEWEEVDELPKTKNRGGFGSTGNS"
BASE COUNT         2104 a         1582 c         1219 g         2228 t
ORIGIN      
        1 cccagcccaa catcccaatc ctcgcccctt actcctcgcc ccgattcaag gtcataataa
       61 taagaaatgg cgccgttatt gtgctgatgc attgctggaa atcggctttg atatttatgg
      121 ttttggcggt tggccacttg atcacaatgg ccaatttgat tatcaattgg ctcaattcaa
      181 cgcgcaactt acaccagacg aatatccccg ttttgctctc ggtgtgggca gtttagaaaa
      241 cattgtccgc ttatatctaa tgggttacca attttttgac tgtgtgctcc cgactcgcga
      301 tgcccgtcac caacgccttt atatttttca aactgatccc caagccctag atttagctaa
      361 catcaaaaaa ctagcaacaa atcatcaatt agacaaaatt tttgattatt tatatattgg
      421 ccggagtaaa tatcaaacag cttttcagca aattagtgaa cactgtgatc gtccaatttg
      481 ccaaaatcaa cagataagta gggcttattt atatcatctt tttaaagtga aagacagcag
      541 tgcttggcgc ttggctactc tccacaattt acgaacttat caactgttaa tggaagcatt
      601 gagcagataa aagcaactca aattcgctca accaaacagt tagtaatttc ttaagcaatt
      661 ttgtcaactt tgttgtcaat ctgtttttgt aaacttagtt agtgaataaa aaaagccacc
      721 aaccagatca aagcaaatct cttctaacct gtcgtccgcg tgaaaaactt tattattctg
      781 ggaaaaacag tttaaataac gccgaactac tggccattat cttaaatagt ggccacaaaa
      841 atttaccagt gctgaatttg gccaaaaata ttttaaaaaa gattccgctc aaccagtggc
      901 acgaaatttt tttattaaaa aatcaccaac gagaacaaca gttatttaaa gatttgcaag
      961 acattcctgg attgggtttt gcccaagcga gcaaaattat cgcggccatt gaactaggtc
     1021 ggcgcagtat cgaacaagcc aagactagtc cgcttcactc tccccaacaa gtttatcact
     1081 actgctctga gttaaaaaac aaaaaacaag aatactgctt agcatttttt ctcaatggtc
     1141 aacaagaatt attgttaaaa aaaattatta ccattggtgg actaaatttt aattatttag
     1201 aaggtcgaga aatttttgaa atcgcctttc aactgcaagc cagcaatcta attttggtgc
     1261 ataaccatcc atcgggaaat ttacaaccat cgctgaatga cttacaagta agtaaaaaat
     1321 tggccgaact ggctgaagcg atgggcatca atttaattga tcatttaatc gtttctagtc
     1381 aaggattttt atccatcaga gaaaagtgtg gcgacttaaa aaaattggac tgccaatttt
     1441 aagatagata aacctgcctt gaaaatctaa gattacttaa tttttctaat agctaactgg
     1501 ccatctgcca agtctaaaag ttcatagccc tgttctttta actctgcccg caaccgatca
     1561 gcttcagtaa aatcctgatc acgacgggct agttggcgtt ttttaattaa ttcttgaatg
     1621 gaatctggta aatcagacaa ttgtaagagg ttttcttgca cacccatttt ttgagcaaaa
     1681 tcttgatcga taattgtttt ggctaaagct tttttgactg cttctagtaa agattcttct
     1741 gttaaaccag ctaggcccaa acctaaaacc tgatcaaatt ccataattaa gcgatataaa
     1801 acatcactgt ctaaatcact ttgcattaat tcccacagca ctgtcagggc ttcattagtt
     1861 tttaagtcat tttctatagc gtcaaaaaaa tcatggtgta gttgcactgc tccaggaaaa
     1921 tcactcagtt ctaactcact gttacgttta ctattgcgca gatctaaaac aaaacgagct
     1981 aattttaaat aggctttctg ggcggcggct aaactatccc aagtaaaatt ttgttcactg
     2041 cgataatgac tcatcaagaa taataaccgc aacgctcttg gcgcaaaacc tctttcaata
     2101 acgtcatcta ttgtgtaaaa gttgttgagt gacttactca ttttttgacc atcaataaac
     2161 aaataattgt gatgaaccca ataacgacta aagggctttt taccagtgac cgcttcagtc
     2221 tgtgcaattt cattagtatg gtgaatatta atgtgatcaa caccgccggt gtgaatatca
     2281 atttgttcgc ctaggtgttc tagggccatg gcactacatt caatgtgcca accaggaaaa
     2341 ctacgctcat gccaggggct gggccaaacc atttgccgat tctcgcccgg tctttcaaat
     2401 ttccacaacg caaaatcagt gggatgtttg cgacctatta ctttatctac tcgctcccct
     2461 tcacgttggg ccattaatac ttgcttggct aacttaggat ctggattaat taacttacca
     2521 taatcatcga tcttactagt atcaaaataa acgccatcgc cctcaatcac ataagtcaaa
     2581 cctttttttt ctagtgccaa aaccatttct aactgagctt gaatatgatc agtggctcgg
     2641 cagcttaaac taggccgcaa aacaccaagc aaatccatac tgtagaaaaa ataatcttca
     2701 aaacgcctcg ccagtgcttc agcaccggta tgatatttct ttgccccttt ttccaattta
     2761 tcttcgccaa catcatcatc actagtcaaa tgaccaacat cagtgatatt ttgcacatgt
     2821 ttaacttcat aaccagcatg acgcagtgtt ctgactaaaa tatcatccaa gacatattta
     2881 cgcaaatgac ctaagtgagt gtaatcatat actgtgggac cacaagcata gagtttaacc
     2941 gatttatctt ccaatgggtg aaattcttca attgattttg aggcggtatt ataaagtttc
     3001 atgcctagct cattctagca ttaaagttta ttctactcca ccaagggtag tgcgctgacg
     3061 ctggatcagt gtttgggctt tttgcttact ccccattccg ctaaaaccac gaaacccagt
     3121 ttccctaccc ttgggaacaa ctaattcttg ggcctcttgt gcttctaatt cttgtctggc
     3181 tttttctttg tctgcttctt cttggcgctt ttttgctaaa ttggcttcgt aaatacgttt
     3241 ggcctcggcc tgttcctcat tagttaatcc ttgccagcga gctgccactc gtttttgttt
     3301 agcttgttcc tttggagttt tgggctgaat atttaacagt gtattgatat caaacatagc
     3361 ttggaaacta tctttaaaat ctttaactgg tcttttcagc agttcttgac ctaaaccagc
     3421 tatggcacgg ggctgagtcg ctattttctc aggggtttga gctaaagccg ttttattggc
     3481 gccttgaata gccgccgcct cttgcaccgc ccgccgttga cccaacacac tactttgttg
     3541 agcaagttgc tcgttatgac cactgtcagc ggcggcggct ttatctaaat gttcttcact
     3601 aatactgcca ccaaaagttt gacgaaaccc accactagtc gttagctggc ttttagcacc
     3661 aataccgaaa cttggtccac cttccatatt tgattaatct aatcttttta actgttcaac
     3721 ttcatgaatt aattgttgag tagcctgttc attggcaggc ccttgagttg aatcttgagc
     3781 cgaattttgg gttaccccat cttgagttga atcttgagtt accctatctt gagtcacatc
     3841 ttgagtattt tctttgcttg atcctgttga taccacttga ggcagctcag ttctcaaatt
     3901 ctcaagagct tgttgatcca acacggcctg ctgttgttca tgtgctttca gttgagcttg
     3961 caattttgct tcttcctcag cattagcttg ttgttgagca gcttgttgtt ctggcgtcag
     4021 ctctccacta gtggcagaag ttgctactga gacgttttcc ttattatcaa ttagtgtttt
     4081 taaagcttgg agcggatctt gattatctgt catatcactt agaataacac aaaaccaagg
     4141 acgataacca ccctctttaa gaagactagg ctgaggacta ggctaatgat taggctaatt
     4201 aaaggctaat gttccaattg tttttcttta cttcttctta atttgcgggt ggctgcgtcg
     4261 actgcatcga caatagcatc ataaaagtca taaccggcta cctcaatcca taaatcttga
     4321 ccggggactt caattttgta tttcaccaaa acatttttac ttttactgct tttcttcgtt
     4381 ttttgctcca aataaactcg aaccgctgta atttcttgcc ctaatttctc taatttttgg
     4441 ctgtgtcgct caataaactc tcgcaaagct ctggtgactt tgagactttt ggcttgaaca
     4501 atcactttca tgatctcccc ctctagcact aaggcttaca acttaataaa atttgtttta
     4561 attgagcatt ttttacaaaa aaacgggtaa ctctgtgaga aaaaagtacc cggtttagct
     4621 ttagcagatg ttgaaccatc tacactttaa tatagcaaaa atagtattaa taaacaagtt
     4681 ttgctataat gtcttttatg acagaaaacc tagatgcaaa caccaattta gttgataaac
     4741 tgaataaagc aaataacaat gaaccaaaca atattactca agagcatcaa gataaatcag
     4801 caccaaaaga acaacatcaa cagttgtcca attggggaaa agtgagaaga tttgtctcgg
     4861 ccgctttaat cggcatcagt ttagcatctt gtcgccaaac tttaactaac gaagaacaga
     4921 gaaacttgga cactttggga gacaatagtc caatggttgc aactgcttac aacatttatc
     4981 tcaacaatga agctcaaaac aatcaaacaa acacccctga ggctgttcaa gcatttaacg
     5041 aacctgaact ttttgaagta cctgcagttg atcaaaccat tttccccgga tcacgggtgc
     5101 aagtttttga aactgatggt tacggtttaa acattcggag tgaggctggt atcaattccg
     5161 ctgtagtgac caatgctgct gagggcacaa tctttaaagt aatcgagtta gtgggagaaa
     5221 aagatggtta tcagtgggcc ttagttaaat cagaacccaa agagggtcaa aatgaaatag
     5281 ttggttacgc agctgttgat tggttacaag tcctaagaga agcacctatc aaataaccat
     5341 tactgataaa acagcagaaa aatttacaaa tgtcttctac cctcaagagc tctcgctaat
     5401 gtcaccccat cagcatattc aatatctgcg ccaatcggca aaccacgacc gattctagta
     5461 atagttagcg ctccactttc aatttttttg gcaaaaaaat catcattttt cagcatttca
     5521 gcaatataca aagcagtcgc ttcgccttcc atggtgggat tggtagctag aatcaattcc
     5581 ttgactatca atttcccaga agaatctctg agtcgtttac tcacatcacg cagataaagc
     5641 tgatcaggac caatattatt gagtggggca atcgaaccat gcaagacatg ataatgaccc
     5701 agatacttac ctgaacgttc aattgctaat aaatcgaggg gttgttctac cacacataat
     5761 aaatcttgat ctcgttgctt gtcttgacag atatcgcaag gatcttgttc ggctacattg
     5821 tgacagacac tacacatcac cgtttccctt ttaagggcca agagcctatc ggcaaaatca
     5881 accaattgac tttctggcgt gtgtaataaa tagaaaacca gacgttgagc agatttcgga
     5941 ccaatgccag gtagggcttc aaatttttcg attagccgac ggacagcttt aggtaacttt
     6001 tgcataattt tctaaaaatg ttttattttg aatattctaa atctacacca gccaaattat
     6061 cttcccaagt gcttagttct tttttggcta aatattgttt aactacttgc caaatattgt
     6121 cctccacttg atcgatactg ggacgagcat caattatcac ccaacgcgct tttttaccat
     6181 gctcttcttg atctttttta gctagcgtca aataaccctg acgcactttt tcgtgaaaat
     6241 ctgtggcctc catatctaaa cgattcacct caccagtgcc atttcttctt ttaagaccaa
     6301 tattaactgg aatatcaagc aaaaaagtaa gatctggata agtttttttg gtggaaatat
     6361 cattaagttg ctcaatcaat tcggtcccaa accctcttac catcccttga taaaccacgc
     6421 tactatcacg agaacgatcc ataattacca ctttacctgc tttgagcgaa ggtaacacaa
     6481 tttctcgata aatttgtgag cgggcagctt gaaatagtag aacttcagta gtaaaagcga
     6541 tgccagtatt tttactagat aaaactactt cgcgaatttg ttcactaatc accgttcctc
     6601 ctggttcgcg caaaaccact acgtctttac ctgcctcaat taaatgattc ttgagacgca
     6661 caatttgggt agtttttccg ccaccttccc caccttcaag agtaatgagc aagccccttt
     6721 tcccattcga ttgcataagt ttgttcctaa tttgcttata tctaacttat atctaatatt
     6781 agtttttatt atatgtaaat tagtggttat tattatatgt aaacggacat ttccctatta
     6841 atcaatttaa ctgttgccag tactaccaaa accgccacga ttttttgtct taggcaattc
     6901 gtccacctct tcccactcta ccttttctct tctgagtacc atcatttgtg ctacgcgctc
     6961 acctttttta atcaacactg attcttgaga aaaattataa acagccgcct gatactcatc
     7021 atcgctacca cagtaatcgc tatcaccaat cccaattcca ttgatcagca ttagacccct
     7081 ctgatgcaaa gagctccgag cagccaccaa cacaaagctg tcggctggaa cct
//
LOCUS       DAOSEW010000030         7019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_9345_length_7019_cov_3.986789, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000030 DAOSEW010000000
VERSION     DAOSEW010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 7019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7019
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9345_length_7019_cov_3.986789"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..499)
                     /locus_tag="PLJ02_01735"
     CDS             complement(143..499)
                     /locus_tag="PLJ02_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOI05170.1"
                     /translation="MSDNRQEEENKKKLFVGNLPFTATQEELQEVFSKFGEITNVNLI
                     TDRMSGRPKGFGFIEYASEEEAQAAIDGMAGQKIGDREVIVNLARPRQPRTNRGGFGG
                     QRNYGGNRSDNRSNRY"
     gene            complement(754..828)
                     /locus_tag="PLJ02_01740"
     tRNA            complement(754..828)
                     /locus_tag="PLJ02_01740"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(793..795),aa:Arg,seq:ccg)
     gene            962..1546
                     /locus_tag="PLJ02_01745"
     CDS             962..1546
                     /locus_tag="PLJ02_01745"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HOI05171.1"
                     /translation="MTKKDFKLAIIVGTNRTNRQSMLVARLIEKAAQSFVGFEPQVVD
                     LLQFHFSQDDQEPALSDPAYKQIIEEADALLIVLPEYNHGYPAALKRALDSEYAIYLH
                     KPVALASVSSGPWGGVRAIESLLPVLRSFGLLISKVDLNFSNVRQLFDNDGNLLDLAY
                     LPRIEKFLTELLWLTQISHWGQKNLNSAGLTKNQ"
     gene            complement(1540..2400)
                     /gene="folD"
                     /locus_tag="PLJ02_01750"
     CDS             complement(1540..2400)
                     /gene="folD"
                     /locus_tag="PLJ02_01750"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007042479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOI05172.1"
                     /translation="MAQLIDGQALSKKIQKRIATEVSTLKFKPGLAVVLVGNDPASQV
                     YVRNKEKACQAVGFYSEKIVLEENITQDKLLAVIKELNNNPKIHGILVQLPLPQHLDE
                     KIVIDNISPEKDVDVFHPYNVGELFLQKRLKNLDELLAPCTPKGIMRMLEEYQIELSG
                     KKVVVLGRSNLVGKPIALMLLASDATVEICHSHTKNLADITAQADILIAAIGQANFVK
                     KNMVKEKAVVIDVGINRTKEGLVGDVDFAAVEKIAAYITPVPGGVGPMTIACLLENTL
                     LLAKKQNQAH"
     gene            complement(2488..4119)
                     /locus_tag="PLJ02_01755"
     CDS             complement(2488..4119)
                     /locus_tag="PLJ02_01755"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOI05173.1"
                     /translation="MQSDIEIAQATKLKPIKEVAQSIGISEQYLEYYGNSKAKISLDI
                     WDKLDQKKDGKLVLVTAMTPTPAGEGKTTTTVGLGQALWSLGKKSMICLREPSLGPVF
                     GIKGGAAGGGYAQVLPMEDINLHFTGDLHAVGAANNLLATLIDNHIHHGNSLQIDPRK
                     ITWRRCVDMSDRSLRSIVIALGRGNGLTRQDGYDITVASEVMAILCLAKDIKDLKKRL
                     EKMVVATNTKGEPVTAKDLQAAGAMTVLLKDAIKPNLVQTVEGSPAFIHGGPFANIAH
                     GCNSLIATRTALKLADIVVTEAGFGADLGAEKFFDIKCRMGKIKPNAVVIVGTVRALK
                     HHGKGVLKDGLANLGKHIENIQKFGLPLVVAINKFDTDTPQEIKLIEKYCEEHGVKAI
                     MSEVWAKGAAGGQDLAKEVLNLVNQENHFKYIYDPQKGLKNSVEEIVSKVYGGDGVIW
                     SAAARQAIKRFEKWHITGLPICMAKTQASISDNPKLLGRPEGFKVKVREIRLSAGAGF
                     FVVIAGDIMTMPGLPKQPASDNIDIDDQGKIVGLF"
     gene            complement(4158..5501)
                     /gene="glyA"
                     /locus_tag="PLJ02_01760"
     CDS             complement(4158..5501)
                     /gene="glyA"
                     /locus_tag="PLJ02_01760"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOI05174.1"
                     /translation="MKTYIPQIKKIDPEMADLIQKECKRQEETLMMIPSENIASEAVQ
                     QAVGSCLANKYAEGYPHKRYYQGQQYVDQIENLTKARAVKAFGVVHANVQALSGSPAN
                     LAVYLGLLKPGDTIMGLDLSHGGHLTHGSKVSTSGIYYHSVSYTLNKDGRMDYEAIEK
                     LAIKHKPQIIVAGITAYPLKINWSKFAKIADKVGAYLMADVSHLSGLIVAGVYPSPAP
                     YAHVITTTTHKTLRGPRGAMIMVTKKGIKKNPDLPKIIDNAVFPRLQGGPHINTIAGI
                     GVALKEAMTPRFKKYGAQIIKNSETLAKELKSKGFHLVAGGSNTHLILIDLKNKGIIG
                     NLAAEALEAANIVLNRNSVPFDTNPPFYPSGIRMGTPGITSRGMKEKEMVKIATWISD
                     IIDDIGRLQKEQKLSFDQVKKSAVRQKLIASSQVIKEVKKEVLKLCQKFPIQAEY"
     gene            5602..6198
                     /locus_tag="PLJ02_01765"
     CDS             5602..6198
                     /locus_tag="PLJ02_01765"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOI05175.1"
                     /translation="MKQQFLDKKTLAIIKEKKELRTQFKLQREQLNETQIKQKSQLIA
                     EKVLNLAEFKKAKKIMCYVSKDNEVATHDLIKKILTMNKTLAVPLIVKKGIMKPAIIN
                     SFAELRLADFNTLQPRTSKFLEDKIDLNLMPALAVTKTGDRLGWGGGFYDRFMMSKQA
                     QLNLVLAFDCQIAEQLPTTEFDQKVDGIITESQLFFLD"
     gene            complement(6225..6827)
                     /locus_tag="PLJ02_01770"
     CDS             complement(6225..6827)
                     /locus_tag="PLJ02_01770"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOI05176.1"
                     /translation="MVTVQGLGEVSVPATSATVSLTLSANSGTSPASIDAVKNKAENI
                     RTLLIASGVLESDISESQIVSYPANLVATGVEGFTSTIQMTAKTTNVAGVGELIGNLY
                     NAGADLVNQPVLNVEDQDKLEEQATKEALKEAKKQINRISLKNFKFIKKMVALSEQSS
                     GTTSTSSVRPELFSQDLSESAEAKGTFKIAKLVTVTYKLW"
     gene            complement(6781..>7019)
                     /locus_tag="PLJ02_01775"
     CDS             complement(6781..>7019)
                     /locus_tag="PLJ02_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05177.1"
                     /translation="KKPVENNSSEAPKKTFFVKKEDVVAKQEAPKKFTPKGDFKKDNN
                     FGSITNCSDYYLAMDFFASDGNSTRSRRSICSSY"
BASE COUNT         2076 a         1418 c         1268 g         2257 t
ORIGIN      
        1 taattaattt ggagtttgag ctcacttttc tattattaat tgtaatttag acaacaaatt
       61 gacaagcaaa acttgtaccg tttagctact aaaaaaccag ccccgaagag ctggtttttt
      121 ctttttaaaa caattgcttg atttaatagc gattactacg attatctgaa cgattaccgc
      181 cgtaattacg ttgaccacca aagccacctc gattagtacg aggttgacga ggtctagcca
      241 aattaacgat aacctctcga tcgccaattt tttgaccagc cataccgtca atagcggctt
      301 gggcttcttc ttcacttgca tattcgatga agccaaaacc cttaggtcgg cctgacatac
      361 gatcagtgat taaatttaca ttggtgattt caccaaattt tgagaaaact tcttgaagtt
      421 cttcttgagt ggcagtgaaa ggtaggttgc caacaaagag ctttttcttg ttttcctctt
      481 cttgtctgtt atcagacatg ttttcctttt tcccttgcaa aaacatttgc aaagataata
      541 atactcaccc taaaattagg gtcatcttgt attttgaact atggaggaaa ttatattgga
      601 cctcgacacg ttttcaattt aagacaggat agattatagc atggaactga gaaaatacta
      661 gtcttgatca agtaactagt aatctcagtc ttaatcaagt aactagcaac cttggtcttt
      721 ttgcaggcag ttctggttct tctcaagttg tcttggtgct tctgaaggga atcgaacccc
      781 cgtctttggt tccggaaacc aacgttctat ccactgaact acagaagcaa attgttaaat
      841 gactttttcc aggttcggca aacacctgat agtcaccatt agttattata caattgacat
      901 ctcgccgaca gctatagtaa tcaacgactg ccgttttcat cattgtacta taatagtcac
      961 tatgacaaag aaagacttta aattagccat tattgtcggc accaatcgca cgaatagaca
     1021 atcgatgctg gtagccagat taatagaaaa agcagcgcaa agttttgtcg gttttgaacc
     1081 tcaagtagta gatttgcttc aatttcactt ttcccaagat gatcaagaac cagcactcag
     1141 tgatccagct tataaacaaa taattgaaga agcagacgcc ttattgattg ttttaccaga
     1201 atataatcat ggatatccag cagcacttaa acgggctcta gatagcgaat atgctatcta
     1261 tttacacaaa ccagtcgctt tagctagcgt gtcgagtggt ccatggggag gagtaagagc
     1321 tatagaatca ctactacctg ttttacgcag ttttggcttg ctaatttcga aagttgattt
     1381 gaatttttct aacgtgaggc aactgtttga taatgatggt aatttgttgg atttggctta
     1441 tttgccacga atagaaaagt ttttaacaga actattatgg ttaacccaaa ttagtcattg
     1501 gggtcagaaa aatttaaact cagcaggcct tacaaaaaat caatgagctt ggttttgttt
     1561 tttggccaac aacaaagtgt tttctagtaa acaagcaata gtcattgggc cgacgccacc
     1621 tggtacgggc gtaatgtaag cggcaatttt ttccactgct gcaaaatcca catcaccgac
     1681 caagccctct tttgttcgat taatgccgac atcaatgacg accgcttttt ctttaaccat
     1741 attttttttc acgaaattgg cctgaccgat agcagcaatc aaaatatcag cttgagcagt
     1801 gatatcagct aaatttttgg tatgagaatg acagatttct acagtggcat cgcttgctaa
     1861 aagcataaga gcgatcggtt taccgaccaa attacttcgt cctagcacaa ccactttttt
     1921 gccactcaac tcaatttgat attcttccaa cattctcata attcctttgg gagtgcaggg
     1981 ggcgaggagt tcatccaggt tttttaagcg tttttgtaag aaaagttcac cgacattata
     2041 aggatgaaaa acatcaacat ctttttcagg actgatgtta tcaatgacaa ttttttcatc
     2101 taaatgttgg ggtagcggta attggactag aataccatga attttgggat tgttgtttaa
     2161 ttccttaata accgctaaca atttgtcttg agtgatattt tcctctaaaa caattttttc
     2221 actataaaaa cctacagctt ggcaagcttt ctctttattt ctcacataaa cttgtgaagc
     2281 tggatcatta ccgactaaaa ccaccgctaa gccaggttta aattttaaag tgcttacttc
     2341 tgtggcaatg cgtttttgaa tttttttcga taaagcttga ccatcaatta attgagccat
     2401 acactcctca ctcttgcccg aacctgagca agttttttat aatcttatta atctcgttta
     2461 gctagaacaa gtagctagaa caaatagtta gaacaaacca actatttttc cctgatcgtc
     2521 aatatcgata ttgtcagaag cgggttgttt gggtagacct ggcatagtca taatgtcgcc
     2581 ggcgataact acgaagaaac cagctccagc acttaagcgg atctctctaa ctttaacttt
     2641 aaaaccttct ggtctgccca gtaattttgg attatcagaa attgaagctt gagttttagc
     2701 catacaaatt ggcaaaccgg tgatatgcca tttttcaaat ctttttattg cttgtctagc
     2761 cgcggcactc caaattaccc catcaccacc ataaacttta ctgacaattt cttcgacaga
     2821 atttttcaaa cctttttgtg gatcgtaaat atatttgaaa tgattttctt ggttgaccaa
     2881 atttaatact tctttagcta aatcttgacc acccgcagct cctttagccc acacttcaga
     2941 catgatcgct tttactccgt gctcttcaca atatttttca attagtttta tttcctgtgg
     3001 agtgtcagta tcgaacttat tgatggctac gaccagtggt aaaccaaatt tttggatatt
     3061 ttcaatatgc ttgcctaagt tagcgagacc atcttttagc acgcccttac catgatgttt
     3121 gagcgctctt accgtgccaa ctataactac cgcattgggt ttgattttgc ccattcgaca
     3181 tttaatatca aagaattttt ccgctcccaa atccgcaccg aaacctgctt cagtcaccac
     3241 aatgtcggcc aatttaagag cagttctagt agcaattaaa gaattgcacc catgggcgat
     3301 attggcaaac ggtccgccat gaataaaagc tggggaacct tctacggttt gcactaaatt
     3361 cggcttgata gcgtctttaa gtaaaacggt cattgctccg gcagcctgca gatctttagc
     3421 ggtgactggt tcacctttgg tgttcgtagc gacaaccatt ttttctaacc gtttttttaa
     3481 atccttgatg tcttttgcca gacagagaat agccatcact tctgaagcaa cagtaatgtc
     3541 ataaccatct tgtctggtta gaccattgcc tcttccaaga gcgatgacaa ttgatcgtaa
     3601 actccgatca ctcatatcga cacaacgtct ccaagtgatt ttacgaggat cgatctgcaa
     3661 actattacca tgatgaatat gattgtcaat taaagtagcc aacaaattat tcgctgctcc
     3721 aactgcgtga agatcaccgg taaaatgcaa attaatatct tccatgggta aaacttgagc
     3781 ataacctcca cctgccgctc cgcccttgat gccaaaaact ggtcctaaag atggttcacg
     3841 caaacaaatc attgatttct taccaaggct ccacaaagcc tgtcccaaac caaccgtagt
     3901 ggtagtttta ccttctccag ctggagtggg ggtcatggcc gtgaccaaaa ctagctttcc
     3961 atcctttttt tgatccaact tatcccaaat atctagacta attttggctt tgctgttgcc
     4021 ataatattcc aaatactgtt cactaatacc aatcgattgc gccacctctt tgatgggctt
     4081 tagtttagta gcttgagcga tttcaatgtc tgattgcatg aatgatcttt ctttttaaga
     4141 atttttgttt tctttaatta atattcagct tggataggga acttttgaca aagtttaagc
     4201 acttcttttt tgacttcttt aattacttgc gaagaagcaa ttaatttttg gcgaaccgca
     4261 ctttttttca cttggtcaaa actgagtttt tgctcttttt ggaggcgacc aatatcatcg
     4321 atgatatcac taatccaagt agcaattttg accatttcct tttctttcat accccgacta
     4381 gtaatgcctg gagtgcccat tctaattcct gaaggataga aaggaggatt ggtatcaaaa
     4441 ggaacgctat tacgattcaa aacgatgttg gctgcctcca aagcctcggc agctaagtta
     4501 ccaataatac ccttgttttt taaatcaatt aaaatcaagt gggtattact gcccccagct
     4561 accaaatgaa aacctttgct ttttaattct ttagctaagg tttcactgtt cttaataatt
     4621 tgggcaccgt attttttgaa acgaggagtc attgcttctt tgagagctac tccaatgcca
     4681 gcaatagtgt tgatatgagg tccaccctgc aatcgtggaa aaacggcatt atcaatgatt
     4741 tttggtagat cgggattctt ttttataccc tttttggtaa ccatgatcat tgctcctcgc
     4801 ggtccgcgca acgttttgtg ggtagtggta gtaatcacat gagcgtaggg cgctggtgat
     4861 ggatagacac ctgcgacaat caagccagaa agatgagaaa catcggccat taaataagcc
     4921 ccgactttat ccgcaatttt ggcaaattta ctccaattaa tttttagagg ataggcagta
     4981 atgccagcga ctattatttg gggtttatgt ttgatggcta gtttttcaat cgcttcgtaa
     5041 tccatacgac catctttatt taaggtataa gaaacggaat gataataaat gcccgaagta
     5101 gataccttag aaccatgagt gaggtggccc ccatgagata aatctaaacc catgatcgta
     5161 tcgcctggtt tgagtagccc taaataaaca gccaaattag ctggagaacc tgacagtgct
     5221 tgcacattag catgaactac accaaaagct ttgactgctc ttgctttggt taaattttca
     5281 atttgatcaa catattgttg accttgataa tatcttttat ggggataacc ctctgcatat
     5341 ttattggcca aacaggaacc cactgcctgt tgcacagctt cagaagcaat attctcggaa
     5401 ggaatcatca ttaaagtttc ctcctgtctt ttacattcct tctgaatgag atcggccatc
     5461 tcaggatcaa tttttttgat ttgaggaata taggttttca tgaagaactc ctttaaatga
     5521 taattgttaa aatggtgcag gctgaataac aatgttatta tatgatcaag cagttattaa
     5581 ttcaagccag taaaatccct tatgaaacag caatttctcg ataaaaaaac gctagcaatc
     5641 atcaaagaaa aaaaagagtt aagaactcaa tttaaattgc aacgtgaaca actcaatgaa
     5701 actcaaataa agcaaaaatc tcaattaatt gccgaaaaag tattaaattt ggccgagttc
     5761 aaaaaggcta aaaagattat gtgctacgta tccaaagata acgaagtagc tactcacgac
     5821 ttaattaaaa aaatattaac aatgaataaa actctcgcgg tacccttgat tgtaaaaaaa
     5881 ggcataatga agccggcaat tatcaacagt tttgctgagt taaggctggc tgatttcaat
     5941 acgctgcagc caagaaccag caaattttta gaggacaaaa tcgatttaaa tttaatgccc
     6001 gctttagctg tgactaaaac tggtgatcgt ttgggttggg gtggaggttt ttatgataga
     6061 tttatgatga gcaagcaagc tcaactgaat ctagttttag ccttcgattg tcaaatagct
     6121 gaacaattgc caaccaccga gtttgaccaa aaagttgacg gaataattac tgagagtcag
     6181 ttattttttt tagattgagt ttgacttaat caatcaaaat atttttacca aagtttataa
     6241 gtcaccgtga ctaacttagc gattttaaaa gtacctttag cctcagcact ttcactaagg
     6301 tcctgagaaa agagttctgg tctaactgag ctggtagaag tagtgccact actttgttca
     6361 ctcagtgcca ccattttctt gatgaatttg aaatttttta agctgattct attaatctgt
     6421 tttttagcct ctttgagcgc ttccttggta gcttgttctt ccagtttatc ttgatcttca
     6481 acatttaaga ccggttgatt gactagatct gcgccagcat tatataaatt accgattaat
     6541 tctccaactc ctgccacgtt agttgtttta gcagtcattt gaatggtgga ggtaaaacct
     6601 tctacgccag tagcgactaa gtttgctgga taagagacga tctgagactc ggaaatatcg
     6661 ctctcaagaa caccactagc gatcagtaag gttctaatat tttctgcctt gttttttacc
     6721 gcatcaattg aagctggtga agtgccagaa ttagcactta atgttaggct aacggtggcg
     6781 ctagtagctg gaacagatac ttcgcctaga ccttgtactg ttaccatcac tggcgaagaa
     6841 atccattgcc aaataataat cactacaatt agtaatactg ccgaaattat tatctttttt
     6901 aaaatctcct ttaggtgtaa atttctttgg agcttcttgt ttagctacaa catcttcttt
     6961 ttttacaaag aaagtttttt taggagcttc agaagaatta ttttcaactg gtttttttt
//
LOCUS       DAOSEW010000031         6887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_9586_length_6887_cov_4.366364, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000031 DAOSEW010000000
VERSION     DAOSEW010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 6887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6887
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9586_length_6887_cov_4.366364"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /gene="tsf"
                     /locus_tag="PLJ02_01780"
     CDS             complement(<1..199)
                     /gene="tsf"
                     /locus_tag="PLJ02_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807254.1"
                     /note="EF-Ts; functions during elongation stage of protein
                     translation; forms a dimer; associates with EF-Tu-GDP
                     complex and promotes exchange of GDP to GTP resulting in
                     regeneration of the active form of EF-Tu; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HOI05178.1"
                     /translation="MERLKMAVNTNLVKQLRQATGARIMDCKKALEEAQGDLELAKKY
                     VQEKGLARAEKTADREAAVGYI"
     gene            complement(202..1131)
                     /gene="rpsB"
                     /locus_tag="PLJ02_01785"
     CDS             complement(202..1131)
                     /gene="rpsB"
                     /locus_tag="PLJ02_01785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOI05179.1"
                     /translation="MAESKTNSTTKQATVSKTSSKTSRKSTAKKVAKVTAKKATDKKT
                     TAEKKTAESIKVPTSKTESTVAKTEKKEVKTKAQTTLPEIDLQEMFEAGVHFGHKISD
                     WHPKMAPFIYGSEGGVHIFDLAKTAEQLQLACQRFYELAKEGKTLLMVGTKRSSREII
                     KEAASEMGCFYIVSRWMGGLLTNFDQLQKSIQRMNDIEKGLESGKFDKYTKYERLQLD
                     KEKTRLERFFVGLQGLKGKPDCVFVVDPEKEKIVVRESLSEGVELIAIADSNADPRKI
                     DLVIPANDDSSKSIKYIVNCLKTAYNAGKTARE"
     gene            complement(1468..1674)
                     /locus_tag="PLJ02_01790"
     CDS             complement(1468..1674)
                     /locus_tag="PLJ02_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05180.1"
                     /translation="MSKEQSQNLNELPEEELRQLLDQARRQENYQRELELCIALYRYE
                     GNPCLASTYIKELTAYLAKIAPKE"
     gene            complement(1687..2298)
                     /locus_tag="PLJ02_01795"
     CDS             complement(1687..2298)
                     /locus_tag="PLJ02_01795"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HOI05181.1"
                     /translation="MQFDHIVFDNDPAQYITPSWQDLDQLSFELAKQVIGSGEKFDLV
                     VALAKGAWPMSRALVDYLAMSNLVSLGIRFYSGINQRLKEPEVYQDLPVKVKGKRILL
                     FDDVADTGESLIFASDYLLEQGAALVKTATLFFKERSAITPDYYASRTNAWIIFPFEI
                     REMSQLLSNNWRKQGLAESAINARLKKLNFNEKIMQYFAKIEK"
     gene            complement(2437..3285)
                     /locus_tag="PLJ02_01800"
     CDS             complement(2437..3285)
                     /locus_tag="PLJ02_01800"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOI05182.1"
                     /translation="MINKPAGLVVNRAQTHHEDTIQDWFVEYLGQDFFTESFINNSRQ
                     HWQLLVPHDFTDQFGTPEEIWLNRQGMVHRLDKETSGALLLAKNPGALVNLLAQFKQK
                     QIAKEYLALCHGRFVLDTGKINAPLARARNNRLRFAISATGKKAETAYEVIQRYWEFD
                     WTTFNNKILKNNPVLSEKQQSKILKKGPQLYQSGFSLVRAKPQTGRTHQIRVHFASMQ
                     HPLLADKIYSGKKRQALDRLWCPRHFLHAASIAFFLPTGPVWQKRQLVEVPLTTDLQA
                     AMSLLE"
     gene            complement(3351..4160)
                     /locus_tag="PLJ02_01805"
     CDS             complement(3351..4160)
                     /locus_tag="PLJ02_01805"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOI05183.1"
                     /translation="MWPTLISIGTFRLQSLTVLAVVALFFTAFVFWRKGKEEHYETVE
                     LFDTFLISGLFGVVMGRLTFVLFNWSLFADNWWQIVNVAQFPGNVPLIALAIAGLCLY
                     FLAKKHKWDPFEVLDFWVTAISLGMVFIYLGFFLAGSYAGQLTTVPWGVVMPGTFEKS
                     HPVQLYFLLFYLVLFNYLMRVEYRYRTFEWYRRGKKTAQSGFLLSVFLIATGLFYLIM
                     TVVSLPGLVVAGRVLDPWVYLVVVLAGIALLINRSGVSFKSNRRRRPQIKF"
     gene            complement(4166..4249)
                     /locus_tag="PLJ02_01810"
     tRNA            complement(4166..4249)
                     /locus_tag="PLJ02_01810"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4213..4215),aa:Leu,seq:caa)
     gene            4351..4575
                     /locus_tag="PLJ02_01815"
     CDS             4351..4575
                     /locus_tag="PLJ02_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05184.1"
                     /translation="MRQLIQQATAIDSVWSIVLRGVIWLIISLIIIISMDNPDADLAS
                     KKMRANLGFFIIFMMLSAGLVYLLFGFKQG"
     gene            complement(4604..5938)
                     /gene="dnaB"
                     /locus_tag="PLJ02_01820"
     CDS             complement(4604..5938)
                     /gene="dnaB"
                     /locus_tag="PLJ02_01820"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOI05185.1"
                     /translation="MATKKIDPKVPPHDLQAEESVLAAILIDKDAILRVSEILVPKAF
                     YSAANRAIYQAMLDLYDNRQPIDVITLKNQLKKHKKLSQAGGVSGISRLSNLLSTSAN
                     VENYAEVVREKYVKRELIALSTDIANLAFDESKEINQILDSAEKSVMEISQTHARKDF
                     LPVKQTLVESFDRLDELQKSGGELRGMPSGFADLDNILAGFQKSNLIILAARPGTGKT
                     AMAVNIAQYLTVVAKKKVGFFSLEMSREELVDRLLTAQADIDAWKLKTGRLDQDDFGR
                     LSDAMGILAEAKLFIDDTPGLSIFEIRTKARRLMMEQNIDMLIVDYLQLAHGANQENR
                     VQEVGEISQGLKNIARELKIPVLALAQLSRAVEQRGERSPQLSDLRESGSIEQDADVV
                     IFLYRKDEDDRESVTLKIAKHRNGKLGDFDLFFRGDRVRFYGMEMRRSQNIA"
     gene            complement(5940..6599)
                     /locus_tag="PLJ02_01825"
     CDS             complement(5940..6599)
                     /locus_tag="PLJ02_01825"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOI05186.1"
                     /translation="MQISYFGHSCFKLKGKHGTLVTDPYSAEGVYGISMPQLTADLVT
                     VSHHHRGHDAVEKIKASANRKKPFIIDFPGEYEVGGISVFGVKTFHDKVGGKERGNNL
                     IFTILIDGITVCHLGDLDHPLSDEQLKNLAPIDVLFLPVGGPSSLMGEEAVKVAQAIN
                     PNYLIPMHYADSAYPKDAPLKKIEDFFQAYGTTVEAQEKLDVEKERLPDEMQLVVLSR
                     T"
     gene            6673..>6887
                     /locus_tag="PLJ02_01830"
     CDS             6673..>6887
                     /locus_tag="PLJ02_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05187.1"
                     /translation="MRKEVLLAILIGASLGLLITFAVYQSRRSVSEAQKDQVQEVLNN
                     HTDNEEVDTSQLAITSPEDNSIVDSQR"
BASE COUNT         2106 a         1461 c         1181 g         2139 t
ORIGIN      
        1 caatgtaacc aacagcagct tcacgatcag cggttttttc ggctcgagct aggccctttt
       61 cttgaacata ttttttagct aactcaagat caccttgagc ttcttctaag gcttttttac
      121 agtccataat tcgggcaccg gtggcttgac gcaactgttt tactaaatta gtatttactg
      181 ccatttttaa cctttccatg actattcacg tgctgtttta ccagcattgt aagcagtctt
      241 taaacaattc acaatatatt tgatgctttt gctagagtca tcgttggcag gaatcaccaa
      301 atcaattttt cgaggatcgg cgttgctatc agcaatagca attaattcca caccttcact
      361 taaagattcc cgcaccacga tcttttcttt ctcggggtct accacaaaca cacaatctgg
      421 tttgccctta agaccttgaa gtcccacgaa aaatcgctcg agtctagtct tttccttatc
      481 taactgcaaa cgttcatatt tggtgtattt atcaaatttt ccagattcaa gacccttttc
      541 aatgtcattc attctttgaa tagatttttg caattgatca aagttagtta gtagaccgcc
      601 catccagcgg gaaacaatat aaaaacaacc catttcgcta gctgcttctt tgataatctc
      661 tcgagacgag cgtttggtac caaccattaa caaagtttta ccctctttgg ctaattcata
      721 aaaacgctgg caagccaatt gcaactgctc agcggttttt gctaagtcaa aaatatggac
      781 cccaccctca ctaccataga taaaaggcgc cattttggga tgccaatcag aaattttgtg
      841 accaaaatgc acaccagcct caaacatttc ttgtaaatct atctctggta aggtcgtttg
      901 cgccttagtt tttacttctt tcttttcagt cttggctact gtcgattcgg tcttagaagt
      961 tggtactttt atggattcag cagtcttttt ttcagcagta gtttttttat cagtagcttt
     1021 tttagcagta actttcgcta cttttttagc cgtacttttt cgactagttt tactactagt
     1081 tttactaaca gtcgcttgtt tagtggtgga gtttgttttg ctctcagcca tatgttcctt
     1141 gtttatttca gatcacatct gaaaagggtc tcggtatcta atcaggaaaa ggagtacctc
     1201 aaccaaaatt tataattagg gtttaaattg tatcatagtt cttttttaaa tccagtcatt
     1261 aaaaccacac tagattatag gacagttttt aaaaacatta tgggatatag actggagtgt
     1321 tattaactac tgaatgcaac aatgccgata ataacgccgg tgagatgtag ttagtcgcaa
     1381 aatcggctaa ctgagcgcct tgttcctcaa gttctcgtgg caaatgactg gcgctggcat
     1441 aaacccaata attaattttt taaactacta ctcttttggg gcgattttgg ctaaataggc
     1501 tgttaattcc ttgatatatg tagatgccaa acaaggattg ccttcgtatc gataaagagc
     1561 gatacatagc tctaattctc gttgataatt ttcctgtcgt cgagcttggt ctaataattg
     1621 cctgagttct tcttcaggta actcatttaa attttgtgat tgctctttgc tcatggctat
     1681 tcctttctat ttttcgatct tggcaaagta ttgcataatt ttttcgttaa agtttaattt
     1741 ttttaatctt gcgttaattg cagactcagc cagtccttgt ttgcgccaat tattcgacaa
     1801 taattggctc atttccctga tctcaaacgg aaaaataatc caggcgttag tgcgactcgc
     1861 ataataatct ggagtaattg ctgaacgctc cttaaaaaaa agtgtggcag tcttcaccag
     1921 agctgccccc tgctctagta aataatcgct agcaaaaatc aatgattctc cggtatcagc
     1981 tacgtcatca aaaagtaaaa tcctcttacc ttttactttg actggtaaat cttgataaac
     2041 ttctggttct tttaagcgct gattgatccc agaataaaag cgaatgccta aactaaccaa
     2101 gttagacata gctaaataat caaccaaagc acgactcatt ggccaagccc ctttggccag
     2161 agcaaccacc aagtcaaact tctctcctga tccaattacc tgcttcgcca attcaaaact
     2221 taattgatct aaatcttgcc aactcggagt aatatattga gctggatcat tgtcaaaaac
     2281 tatgtgatca aattgcattt tttaattctt taataaaaaa agagcgctca acttttctta
     2341 ttctcttggt ttccctcttg actcaaaagt caaaattcgt tgcttgggaa tacgaaaagc
     2401 ttaacgctct ttcgcactct ttttgcttaa tgtcgcttat tctaacaaag acattgcggc
     2461 ttgtaaatca gtagttaaag gaacttccac taactgtctt ttttgccaaa ctggcccagt
     2521 gggcagaaaa aaagcgatgc tggcagcatg caaaaaatgc ctagggcacc agagtctatc
     2581 tagagcttgg cgcttcttac ctgagtaaat tttgtcagca agcaaaggat gttgcattga
     2641 ggcaaaatga acccgaattt ggtgggtgcg gccagtttga ggtttggctc ttaccagaga
     2701 aaaacccgat tgataaagtt gtggaccctt cttaagaatt ttactttgtt gcttctctga
     2761 taaaactgga ttatttttta aaattttatt gttgaatgtt gtccaatcaa actcccaata
     2821 tctctgaata acttcatagg ccgtttccgc ttttttccca gtggcactaa tggcaaaacg
     2881 caaacgattg tttctagccc gagccaaagg cgcgttgatt ttaccagtat caagcacaaa
     2941 acgaccatga caaagcgcta gatattcttt cgctatttgt ttttgtttaa attgagctaa
     3001 aagattaact agtgcaccag gattttttgc caataataaa gctccagaag tttctttatc
     3061 taaacgatgc accatccctt gtctattaag ccagatttcc tctggagtac caaattgatc
     3121 tgtaaaatca tgaggaacta acaattgcca gtgttggcga ctgttgttaa taaaactttc
     3181 agtaaaaaaa tcttgcccaa gatactccac aaaccagtct tgaatggtgt cttcgtgatg
     3241 agtttgcgct cgattaacca ctaacccggc cggtttattg atcactaata gattgtcgtt
     3301 ctcaaaaatt atttggatct gtttgctata cattaattat caatcttaaa ctaaaatttt
     3361 atctgaggtc gcctccgccg attggactta aatgagacac cagaacgatt aatcagcaac
     3421 gctatcccag ctaaaacaac cactagataa acccaaggat caagcactct gcctgcaact
     3481 accagtcctg gcaaagaaac cacagtcata atcaagtaaa aaagacctgt tgcaattaaa
     3541 aacactgata gtaaaaatcc cgattgagca gtttttttac cgcgtctgta ccactcaaaa
     3601 gtacgatagc ggtattcaac ccgcattaaa taattaaata aaactaaata aaataaaagg
     3661 aaataaagtt gcactggatg acttttttca aatgttcctg gcatcactac tccccaaggc
     3721 actgtggtta actgaccagc atagctacca gctaagaaaa atcctaaata aataaacacc
     3781 attcccaaac taatagctgt tacccaaaaa tctaacacct caaaaggatc ccatttatgc
     3841 ttcttagcta gaaaatacaa acacaaacct gcaatcgcca gagctattag aggcacattg
     3901 ccgggaaatt gcgccacatt aactatttgc caccaattat ccgcaaaaag tgaccaatta
     3961 aataaaacga aagttaatcg acccatgact accccaaaca atccagagat caaaaaagtg
     4021 tcaaacaatt caactgtttc gtaatgctct tctttaccct tgcgccaaaa aacgaaggct
     4081 gtaaaaaaca aagccacaac cgccaacacc gttagcgatt gtaaacgaaa agtaccgata
     4141 ctaatcaaag ttggccacat aaatgtgccg agagagggaa tcgaaccctc atgaagttgc
     4201 cttcataggt ttttgagacc tacgcgtcta ccaattccgc catctcggcg atgactatta
     4261 gttgaagctt gtatatttta ccgtaaaaag acttgttttt gtaaggaaat tgcgtcatga
     4321 taataaacgc aacaaggaac aatggatagt gtgagacagt taattcaaca agcaactgcc
     4381 atcgacagcg tgtggtcgat tgttttgcgt ggcgtaattt ggctaattat ttctttaatt
     4441 attatcatta gtatggacaa ccccgacgct gatttggcta gtaaaaaaat gcgggccaac
     4501 ctggggttct ttattatttt catgatgctc tcagccggat tagtctatct gctttttggt
     4561 tttaaacagg ggtaaatatt gctcacatca cactaaacaa aatttaggcg atattctgtg
     4621 atcgtctcat ttccatacca taaaaacgca ctcggtcacc tctaaagaac aaatcaaaat
     4681 caccaagctt accattacga tgtttagcaa tttttagggt aactgattct cggtcatctt
     4741 cgtccttgcg atacaagaaa atcacgacat ctgcatcctg ctcaattgat ccagactcac
     4801 gtaaatcgga caattgtggc gagcgttcgc ctcgttgctc aactgcacga gacagttggg
     4861 ctaaagctaa aaccggaatc tttaactcgc gagcaatatt ttttagacct tgagaaattt
     4921 ctcccacttc ttgtacccga ttttcttgat tagcaccatg agccaattgc aaatagtcga
     4981 cgattaacat atcaatattt tgttccatca ttagacgacg agctttagtg cgaatttcaa
     5041 aaatactcaa accgggagta tcatcaataa ataatttagc ttcagctaaa atgcccatgg
     5101 catcagatag ccgaccaaag tcatcttgat ctaaacgacc agttttcaat ttccaagcat
     5161 caatatctgc ttgagcagtt agtaaccgat caactaattc ttcccgactc atttccaaag
     5221 aaaaaaagcc cactttcttt ttggctacaa ccgtcaaata ttgggcaata ttgactgcca
     5281 tcgctgtctt accagtacct gggcgagcag ctagaataat caggttagac ttttgaaaac
     5341 cagctaatat attatctaaa tcagcaaaac cagagggcat acctctgagt tctcctccac
     5401 tcttttgtaa ttcatctaaa cgatcaaaac tttcaaccag ggtttgttta actggtaaaa
     5461 aatctttacg agcgtgagtt tgcgaaattt ccattactga tttttcagca ctatctaaaa
     5521 tttgattaat ttccttagat tcatcaaaag ccaaattagc tatatcagtt gatagagcaa
     5581 ttaattcgcg tttaacatat ttttctctaa caacctcggc gtaattttct acattagcgc
     5641 tagtggataa taaattggat agacgactga tgcccgatac accaccagct tgacttagtt
     5701 ttttatgttt tttgagttga tttttaaggg taatgacgtc aattggctga cgattgtcat
     5761 ataaatcaag catcgcttga taaatagctc gattggctgc gctataaaaa gctttaggca
     5821 ccaaaatttc tgaaaccctg agaatggcat ctttatcaat taaaattgct gccaagactg
     5881 attcctcagc ctgtaaatca tgaggtggca cttttggatc aatttttttt gtagccatac
     5941 tatgtacgag ataaaactac taactgcatt tcgtcgggca agcgctcttt ttcaacatct
     6001 aacttttctt gagcttcaac tgtagtgcca taagcttgaa aaaaatcttc gatttttttc
     6061 agtggcgcat ccttgggata agcactgtca gcataatgca tcggtatcaa ataattagga
     6121 ttaatcgcct gagctacttt gaccgcttct tcacccatca gtgaacttgg accaccaact
     6181 ggtaaaaaca aaacatcaat tggtgctaaa tttttcagtt gctcatctga taatgggtgg
     6241 tccagatcac ccaaatgaca aacagtaata ccatcaatta aaatggtaaa aattaaattg
     6301 ttgccgcgct ctttacctcc aactttatca tgaaaagttt tgacaccaaa gacactgata
     6361 ccacccactt catattctcc agggaaatca ataataaatg gttttttgcg attagctgaa
     6421 gctttaattt tttcaacagc atcgtgacca cgatgatgat gagaaacggt cactaagtcg
     6481 gcggtaagct gaggcatact aataccataa actccttcgg cactataagg atcagtgacc
     6541 agtgtaccgt gtttaccttt taacttgaaa caagaatgac caaaatagct aatttgcata
     6601 atcgcatatt accgttaaag ttgacttttt acaagattta acataggatg actcttcaat
     6661 tataatagtc atatgcgaaa agaagttctt ttagcaatct taatcggggc cagtttaggt
     6721 ctgttaatta cttttgctgt ttatcaaagt cgtcgtagcg ttagtgaagc gcaaaaagat
     6781 caagtccaag aagtgctgaa taatcatact gataatgaag aggtagacac tagtcaactg
     6841 gccattactt ctccagaaga taattcgatc gttgatagtc agcgttt
//
LOCUS       DAOSEW010000032         6667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_10008_length_6667_cov_4.690563, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000032 DAOSEW010000000
VERSION     DAOSEW010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 6667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6667
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10008_length_6667_cov_4.690563"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="PLJ02_01835"
     CDS             complement(<1..1005)
                     /locus_tag="PLJ02_01835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOI05188.1"
                     /translation="MKVLQISQYYAPHVGGVEKHVAKINQQLIKNGYQVTVLAGTDNH
                     DLAKAAIIDQVQVYRLDNRLSSCYKFKIWRQVIKQAQLVKEADIIQLHDVFWWIIPLY
                     PFIFHKLFITFHGHESSDKPDQKAIFWHRLANKLCRGSIAVGGFHEKYYQVKADYVID
                     GASDLEQIPGKKKSAKKIIFVGRLAPDTGILQYLEALNILKDQQLNYHLDVYGDGQLM
                     NQAKHYVKQHQLDVIFHGTVAEAEQLFSHYEIAFVASYLSICEALKQNCRVIAHYSND
                     FIKDYLSMSIYRDYILLAKNAPAIAQAIIKPAKTKKLDKKLSNSLTWPVIANTYQKLW
                     Q"
     gene            1017..1958
                     /locus_tag="PLJ02_01840"
     CDS             1017..1958
                     /locus_tag="PLJ02_01840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOI05189.1"
                     /translation="MPKSDYKKEMLSLIVTVFNEADTIEDFLLSVQQQSLAPTELIIV
                     DAQSTDNTVNLIKKFAKKTSLNLKLLVKAGNRSVGRNFAARHSRGTYLAITDAGCILD
                     KNWLQELWRKQQTTDAPVVAGYYQGLANTAFEEATIPYFLVMPERVRDGKFLPATRSM
                     LIKKSLWLEMGGLNEKLSISEDYHLANRLKNKGIKIAFSRKAIVYWLPPSNIKQFFQK
                     IFAFAKGDAATALIRPKVYLIFLRYLIFLAILVFFPNLYLIFSLIFLYLLWAMMKNLK
                     YCPRSFFYLPMLQVVADVAVMIGTSVGRFQANLASKD"
     gene            complement(1931..2713)
                     /gene="murI"
                     /locus_tag="PLJ02_01845"
     CDS             complement(1931..2713)
                     /gene="murI"
                     /locus_tag="PLJ02_01845"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOI05190.1"
                     /translation="MKIAIFDSGAGGLTVLKTFLNLGCATDFIYFADYDHLPYGEKPA
                     AEVSQYVLNSVDFLAKQQIDALVIACNTATSLAIGELRNQKRFNFPIFGMEPAVKLAV
                     DSLAKQKREKKPTVLITATKITAKSQQLAKLKERFSQQANFSTVVLGELVRLVENNNF
                     NQQIIDQYLRKSIEQKQNFDAIVLGCTHFSFFKENFQKLFGNSLILDGNLGTVTHVIQ
                     QLKLDCSPDAINPQPPKIDFYFSQKRNLALAKKYQSLLAKLA"
     gene            complement(2723..3616)
                     /locus_tag="PLJ02_01850"
     CDS             complement(2723..3616)
                     /locus_tag="PLJ02_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05191.1"
                     /translation="MLIEKAKLKIANYFYQEKPDLAEIKEADIVSFNYQLEGGIYIPE
                     KVAWFGDLTGIEKAEDYILSKRARKSLKKTQEFFQDAGFKLDVREMDENLFEEFKKLY
                     QQTTMRKERAIIYDLETSILGRIKVGKKCYIAGLYKENILRSGLVFSINEDSAVVSFG
                     AKERFNKIRGGVGAILEYQLIKFALEKKLKSISHGQANNPAGLFNTAGLFEFKARYGF
                     TAFPTGRWQTLFIKNEQIALGDLVFVTIYNNQLAYLVISDDDLSNLEKKYRTREIKNI
                     VKIDKQSFYLNQKTSLNKIAL"
     gene            3684..4520
                     /locus_tag="PLJ02_01855"
     CDS             3684..4520
                     /locus_tag="PLJ02_01855"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOI05192.1"
                     /translation="MPKFSLDFVQSGVNQYWFFPIPQSNIYQKIENLSIPQLAQISEL
                     DQDNQLLIVKADRRASMRFLYQAQAIKIDWANVRLKKSQKAGDFYRDQYVNAFDPKMQ
                     ELATKWQNQENDPKKICEIFYEKTLEYLSYGRAIKGLHPYSQAFQERVTDCGGFATFL
                     LTLLETQGIVGRLAVGYLLKNNYQQKIKNILNLTYHWSDLTMHAWLELQTAGGSWIPL
                     DPAVDWRYRHGQSQRFAAFANLPADRLLISYGHNHQLTYFDQEHYWPILQHPQLIKVK
                     NA"
     gene            4513..5727
                     /locus_tag="PLJ02_01860"
     CDS             4513..5727
                     /locus_tag="PLJ02_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOI05193.1"
                     /translation="MHNLNFASNENFAEARKALPLLLQKNQGQSGQASEQLQKTVAEK
                     FKVEANQVFFTLAARSALYLFLKSLNLAKNSEVLVQAFTCEAVVLPVLAAHLKPQYVD
                     IEEQTYSMNFNDLVSKIGEQSRVLILQHSFAMLPRDREKIFQLAKKHQLIVIEDLAHG
                     FAPELLKDQTNPSSKLLSFGRSKFFSAVYGGAIIVEKKLINQQFKDSITQLEPVNNQF
                     IRQALLYKICTPMMKSTYHFGGKLFHALFSYLGIFHQEISSKEKEGDYDEWLNKALPN
                     VFAQLLLEQIENYPATHAHRQQIAQLYWQEFPQQMRKDNLPSLRYPILINQADLFLAK
                     MAAKGYILGNWYRQVVAPPGLDLVKVKYQAGSCSQAERMSRAVVNLPLNISLKEAEKL
                     LTVITNVKKNFN"
     gene            5705..>6667
                     /locus_tag="PLJ02_01865"
     CDS             5705..>6667
                     /locus_tag="PLJ02_01865"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HOI05194.1"
                     /translation="MSKKILIELVDDFELWERYLDQFPEANFLQSSSWKTFQGKMGKK
                     VWAVRLYDSKQPKNKTSLALALVVAENAKRAKYLTIAGGPLLNWQNKDRQLIIQSLLD
                     YLKKIAEDEKISFLRLRPQVLSTPELHNFFKQLGFQQSPMHLTADLTLQLDLSQSEEA
                     LLAQMRKNTRYEIRRAQKIGISTRFSKNPADIQAFYEAQLDLAKRQNFVPFSYQFLYQ
                     QFLAFLEHDQVALIHSYYQDQLLASAFVIFYRQEAVYHYGISTLANLKLPGSYACQWA
                     AIREAKARGLSTYNFWGISPANESQHRFAGVSLFKRGFGGKEVAY"
BASE COUNT         2097 a         1170 c         1205 g         2195 t
ORIGIN      
        1 ttgccataat ttttgatagg tgtttgctat cacgggccaa gtgaggctat tactgagctt
       61 tttatctaat ttttttgtct ttgcaggctt gattattgcc tgagctattg ctggagcatt
      121 cttagccagc aagatgtaat ctcgataaat agacatgctt aagtaatctt taatgaagtc
      181 atttgaataa tgagcgatca ctcgacaatt ttgttttaag gcctcacaga tactcagata
      241 actagccaca aaagcaattt cgtaatgact aaataattgc tcggcctcag ccactgtacc
      301 atgaaaaatg acatctaatt ggtgctgctt cacataatgt ttggcctggt tcattaattg
      361 gccatcacca taaacatcta aatgatagtt gagctgttga tctttcagga tgttgagtgc
      421 ctccagatat tgtaaaattc ctgtatctgg ggctaagcgc ccaacaaaga tgatcttttt
      481 agccgatttt ttcttgcctg gtatctgctc aagatcacta gctccatcaa tgacgtaatc
      541 agctttaacc tgatagtatt tctcgtgaaa accaccaacg gcaatactgc cccgacacag
      601 tttattggct aatcgatgcc aaaatatggc tttttgatca ggcttatcac ttgattcgtg
      661 accgtgaaaa gtaataaata gtttgtgaaa aataaaaggg tagagaggga tgatccacca
      721 aaaaacatcg tggagctgaa taatatcggc ttctttgaca agttgtgctt gcttgattac
      781 ttgtcgccaa attttaaatt tataacaact gctcaaacga ttatctagac ggtagacttg
      841 aacctgatca attattgcag ctttggctaa atcatgattg tcagtgccag ctaaaactgt
      901 cacttgatag ccatttttta ttaattgttg attaatttta gccacatgct tttccacccc
      961 gccaacatgc ggagcgtaat actggctaat ttgtaggact ttcatataat aactctgtgc
     1021 ctaagtctga ttataaaaaa gaaatgcttt ccttgatagt gacggttttt aatgaggctg
     1081 acaccattga agatttttta ctcagtgtgc aacaacaaag tttagctcca actgaattaa
     1141 ttatagttga tgctcaatca acagataaca cggttaattt aatcaaaaaa tttgccaaaa
     1201 aaacctcact caatcttaaa ttattggtta aagctggcaa tcggagtgtc ggacgcaatt
     1261 ttgctgctag acacagtcgt gggacttatc tagcgattac cgacgccggt tgcatcttag
     1321 ataaaaattg gttgcaagag ttatggcgaa aacagcaaac tactgatgct ccagttgttg
     1381 ctggttatta tcaaggtcta gccaacacag cttttgaaga agcaacaatt ccgtattttt
     1441 tagtcatgcc agaacgggtg agagatggaa aatttttgcc agccacccgt tccatgttga
     1501 taaaaaagtc attgtggctt gagatgggtg gactgaatga aaaattgagt ataagcgaag
     1561 actatcattt ggctaatcgc ctaaaaaaca agggaattaa aatagccttt agtcgaaaag
     1621 ctatcgttta ttggttacca cctagcaata ttaagcaatt ttttcaaaaa atctttgctt
     1681 tcgccaaggg tgacgccgcc actgctttga tccgacctaa agtttaccta atttttttgc
     1741 gctacctaat ttttttagca attttagtat tttttcctaa tctttactta attttttctt
     1801 taatctttct gtatttattg tgggctatga tgaaaaactt aaagtattgt ccgcgatctt
     1861 ttttctattt gcccatgctc caagtagttg ctgatgtggc cgtcatgatc ggcactagcg
     1921 ttggtcgatt tcaagctaat ttggctagta aagattgata ttttttggct agtgctaagt
     1981 ttcttttttg ggaaaaataa aaatctattt ttggtggttg tggattaatt gcatctggtg
     2041 aacaatctag ttttaattgc tgaatcacgt gcgtaaccgt acctaaatta ccatcaagta
     2101 tcagactatt accaaataac ttttgaaaat tttctttgaa aaaggaaaaa tgagtgcaac
     2161 ccagtacgat tgcatcaaaa ttttgttttt gttcaatgct ttttcttaaa tattgatcaa
     2221 ttatttgttg attaaaattg ttgttttcca ccaatctcac caattcaccc aacactacag
     2281 tactaaaatt agcttgctga ctaaaacgct ctttgagttt ggctagttgt tggcttttag
     2341 cagtaatctt ggttgccgta attaaaactg ttggtttttt ctcccttttt tgtttggcca
     2401 aactatcaac ggccagttta actgctggtt ccataccaaa aatgggaaaa ttaaatcttt
     2461 tttgatttct cagctcgcca atcgctaaac tagtagcggt gttgcaagca attaccagag
     2521 catctatttg ttgtttcgct aaaaaatcaa cactattcag aacatattga ctgacttcag
     2581 cagctggttt ttctccataa ggcagatgat cataatctgc aaaataaata aaatcagttg
     2641 cacaaccaag gttaagaaaa gtttttaaaa cagttaaacc gccagcgcca gaatcaaaaa
     2701 tagcaatctt cattaattgt gattataacg cgattttatt taaactagtt ttttggttaa
     2761 gataaaaact ttgtttatcg attttgacaa tgtttttaat ttcgcgagta cgatattttt
     2821 tttctagatt acttaaatca tcatcgctga tgactaaata ggctaattga ttattgtaaa
     2881 tggtgacaaa aactaaatca cctaaagcaa tttgctcatt tttaataaat aaagtttgcc
     2941 agcgaccagt ggggaaagca gtaaaaccat agcgagcttt aaattcaaat agtccagccg
     3001 tattgaataa accagcggga ttattggctt gcccatgact aatactttta agtttttttt
     3061 ctaaggcaaa tttaattaat tgatattcta aaattgcccc aacccccccg cggattttgt
     3121 taaatctttc tttagcacca aaagaaacga cagcactatc ttcattaata gaaaaaacca
     3181 aaccggaacg caaaatattt tctttataaa gtccagctat ataacatttt ttaccaactt
     3241 taattctgcc taaaattgag gtttctaagt cataaataat ggcccgttct ttacgcatgg
     3301 tggtttgttg ataaagtttt ttgaattctt caaataagtt ctcatccatt tctcgaacat
     3361 cgagtttgaa accagcatct tgaaaaaatt cttgagtttt ctttaaagac tttctagctc
     3421 ttttggacag aatgtagtct tcagcttttt cgatcccagt caaatcacca aaccaggcaa
     3481 ctttttctgg aatataaatc ccaccctcta gttgataatt aaaagaaaca atatctgctt
     3541 ctttgatttc agctagatct ggtttttcct gataaaaata attagctatt ttgagtttgg
     3601 ctttttcaat taacattaag cctattatac tttagagaag gtttttgcag tagtgatctc
     3661 tcttatagta tgataactag ttgatgccaa aattttccct agactttgtg caaagcggag
     3721 taaatcaata ttggtttttt cccatacctc agtcaaatat ttatcaaaaa attgagaact
     3781 taagcattcc tcaattggct caaatcagtg aattagatca agacaatcaa ttactgattg
     3841 tcaaagccga ccggcgcgca agtatgagat ttttgtatca agcccaagca attaaaattg
     3901 actgggctaa tgtcagatta aaaaaaagtc agaaagccgg tgatttttat cgagatcaat
     3961 atgtcaatgc ttttgatccg aaaatgcaag aactcgccac aaagtggcaa aaccaggaaa
     4021 atgatcccaa aaagatttgc gaaatttttt atgaaaagac ccttgaatat ttaagttacg
     4081 gacgagcgat taaaggattg catccatact ctcaagcttt tcaagaaaga gtgactgatt
     4141 gtggtggttt tgcgactttt ttattaactt tattagaaac tcaaggcatt gttggacggt
     4201 tggctgttgg ttatttactc aaaaataatt atcaacaaaa aataaaaaat attcttaatt
     4261 tgacttatca ttggtccgat ttaaccatgc acgcttggtt ggaattacaa acagctggtg
     4321 ggtcttggat tcctcttgat ccagcagtag attggcgtta tcgacatggt caaagtcaac
     4381 gttttgctgc ttttgccaac ttacctgcag atcgtttatt aatcagttat ggtcataatc
     4441 atcaactgac ttattttgat caagaacatt attggccaat tttacagcat cctcaattaa
     4501 ttaaggtaaa aaatgcataa tttgaatttt gcaagtaatg aaaatttcgc tgaagccagg
     4561 aaggctctgc cgttattgtt gcaaaaaaac caggggcaaa gtggacaagc ttcagaacaa
     4621 ttgcaaaaaa ctgtggctga aaaatttaag gttgaagcaa atcaagtatt ttttacttta
     4681 gcggcccgaa gcgcccttta tcttttttta aaaagtttga atttagcaaa aaatagcgaa
     4741 gtgttagtgc aggctttcac ctgtgaagcg gtggttctgc cagttttggc agctcattta
     4801 aaaccgcagt atgttgacat tgaagagcag acttattcaa tgaattttaa tgatttagta
     4861 agcaaaattg gtgagcagag tcgagtgtta attttgcaac acagttttgc catgttacct
     4921 agagatcgag aaaaaatttt tcaattagca aaaaaacatc aattaatagt aattgaagat
     4981 ttggctcacg gctttgctcc agaattgtta aaagatcaaa ccaatccaag ttctaaactt
     5041 ttgtcttttg ggcgttctaa attttttagt gcagtttatg gaggagcgat cattgtggaa
     5101 aaaaaactaa tcaatcaaca attcaaagat agcataactc aattggagcc agttaataat
     5161 cagtttattc gtcaagcact cttgtataaa atttgcacac caatgatgaa aagtacttac
     5221 cattttggtg gtaaattgtt tcatgcctta tttagttatt tagggatttt tcatcaagag
     5281 attagttcaa aagaaaaaga gggagattat gatgagtggt taaacaaagc cttgcctaac
     5341 gtttttgctc aattattgtt agaacaaatt gaaaactatc cagctacgca tgctcatcgt
     5401 cagcaaattg cgcaacttta ttggcaagaa tttcctcaac aaatgcgaaa agataattta
     5461 ccaagtttac gctatcctat actaattaat caagcagatt tatttttagc caaaatggca
     5521 gcaaaaggat atattttggg caattggtat cggcaagtgg tagccccacc aggattagat
     5581 ttggttaaag ttaagtatca ggcgggcagt tgttcgcaag cagagagaat gagtcgggca
     5641 gtggttaatc taccacttaa tattagtctc aaagaagccg aaaaattatt aacagttatc
     5701 accaatgtca aaaaaaattt taattgaact agttgacgat tttgagttat gggaacgata
     5761 tcttgatcaa tttcccgaag ctaatttttt acagtccagt tcttggaaaa cttttcaggg
     5821 gaaaatgggt aaaaaagttt gggcggtaag attatatgat tcaaagcagc caaaaaataa
     5881 aaccagttta gctctcgctc tcgtcgttgc cgaaaatgcc aaacgtgcca aatatttaac
     5941 tattgcaggg ggaccattgt tgaattggca aaataaggat cgccaattaa taattcagag
     6001 tttattagat tatttgaaaa aaatagctga agacgaaaaa atttcttttt tacgtcttcg
     6061 tccccaagtt ttgtctactc cagaattaca taattttttc aaacagttgg gttttcagca
     6121 atcgcctatg catttaactg ccgacctgac tttacaatta gatttatctc aaagtgaaga
     6181 agcgttactg gctcaaatgc gcaaaaacac ccgttacgaa atacggcgag ctcagaaaat
     6241 tggcatatct acccgtttta gtaaaaatcc agcagatatc caagcttttt atgaggccca
     6301 gctggatttg gctaaaagac aaaatttcgt acccttttct tatcagtttt tatatcaaca
     6361 atttttagct tttcttgagc atgatcaagt ggctcttatt catagttatt atcaagatca
     6421 gttgttggcc agtgcctttg tgatttttta tcgccaagag gcagtctatc actatggtat
     6481 ttcgactttg gctaacctta aattacctgg aagctatgcg tgtcagtggg cagctatcag
     6541 agaagccaag gccaggggtt taagtactta taatttttgg ggaatttcgc ccgccaatga
     6601 atctcagcat cgttttgctg gtgtcagttt atttaaacgc ggatttggag gtaaagaagt
     6661 ggcttat
//
LOCUS       DAOSEW010000033         6623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_10112_length_6623_cov_3.984470, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000033 DAOSEW010000000
VERSION     DAOSEW010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 6623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6623
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10112_length_6623_cov_3.984470"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PLJ02_01870"
     CDS             complement(<1..244)
                     /locus_tag="PLJ02_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05195.1"
                     /translation="MLLLIFKLFSWRIINHALLILILIQLILILLLCLMLLIIFAFYR
                     YLNLTKQINSLIKYQEIYCEKTVALIEVAKLSLFSIN"
     gene            complement(225..1190)
                     /locus_tag="PLJ02_01875"
     CDS             complement(225..1190)
                     /locus_tag="PLJ02_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05196.1"
                     /translation="MAVYFNDLDHAVFFTLVYSDYFNYPLKKTEIYQRLPKVWDWDFL
                     TGVKLKSDRFKIDKNSEKFTKSLEKLEKAKKIAKLKVADDDYYFLTNRQQLVKLRLNK
                     EKIAYNRRAVILQASQYLLRFPSIQALGLTGASAMNNASLADDLDFCIIVKKNTLWLT
                     RLVVIVVAKILGKRPQIDAHAKNENKQAWCFNLWLDESSLNIAQRGFSIYQAYELRQM
                     DFLFDKASIQKKLWLQNWQFAELLDLNLPNNDLSSDKDNLIDYFLWPINGFLYLIQKI
                     YRRLAFGKEDFSLNLHQAHFNHIGRQDKIFKSIKKKLQSNAFTDL"
     gene            1237..2004
                     /locus_tag="PLJ02_01880"
     CDS             1237..2004
                     /locus_tag="PLJ02_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOI05197.1"
                     /translation="MKIYSWNVNGIRSVAKNGFVAWLTKEKPDILCVQETKAHPDQLS
                     FDLKMIDHYRVDFASAHKAGYSGVATYSIPEPNKVQIGLNQQRFDQEGRAIVSYYPNF
                     ILLNIYFPNGKASQERLQYKMAFYQYLLAYLPDLKKVNQNIIITGDVNTAHQEIDLAR
                     PKANEHISGFLKEERAWIDQLLNSGFIDSYRYFYPQQKDAYTWWSQRTAARKRNVGWR
                     IDYFFVSQSLKKNLQAATIHREVPGSDHCPISIELKF"
     gene            complement(2065..3195)
                     /gene="coaD"
                     /locus_tag="PLJ02_01885"
     CDS             complement(2065..3195)
                     /gene="coaD"
                     /locus_tag="PLJ02_01885"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOI05198.1"
                     /translation="MTRALYALSGDPITHGHLDIITRAAKAFDQLIVAIGVNRLKNYL
                     FTLEEREEMARRSLVNLKNVKVMSFTGLLVDFAYENNVDVIVKGVRTNQDFEYEQNLH
                     LMGESQRLGIDTFLLFTKPELSHISSSAVKEIQKDQGLIQDYVSPYVKQCLEKKLSKQ
                     YIVGLTGEIGSGKSYVGEQLVQLAKKDKISAHNIELDLLAQQIQEDLSEEKYELVRQE
                     IIKNFGQEVAKKDGRIDRKALGEIVFADSEKLNKLNKIMRTPILVRLRKELKNKEGLI
                     ILNAALLAETQMMDLSNFNLILLDVNEQTQAQRLIKRGLSQEQIKRRLASQYDFAKKK
                     KIIEQAISDHQYGRLFILDNSVDDNKQGIADLWQELKEYFSL"
     gene            complement(3203..5290)
                     /locus_tag="PLJ02_01890"
     CDS             complement(3203..5290)
                     /locus_tag="PLJ02_01890"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOI05199.1"
                     /translation="MNFQALFQPQAIAIIGASSREKTVGNDVVKNLVNQGYAGKIYPI
                     NPKIDELYGLKVYHDIKDLDQKIDLVVVAIPAKFVASAIDEAASKGAQAAIVISAGFK
                     EAGNLALEQELAATCRRHGIILVGPNCLGVINAEHKMNASFAAIMPEVGNVAFMSQSG
                     ALCTAVLDYAQELGLGFSKFISIGNKAALDELALIKYLHEDPQTKVICLYAEALDNAT
                     AIIQTLKELNRGKEPKPVIVLKSGKTAAGAGAIASHTGSLSGGDNAYQALFNQAGIIR
                     AQSVSELFDLASIFSNNPLKEIKTVTIITNAGGPGVLTTDAVIENGLEMAKLEEQTIK
                     KLKSFLPAAANTHNPVDVLGDAVGEVYEKTLAVIGDDRQTDAILVLLTPQTMTEPVKT
                     AQAIVNLRKKTDKVIAVSLMGRDLVADGVKILKDNKIANTTFPESAALGLGALAKFVA
                     WTKIKEDKPLVFNDVDRERVAQIFKKAKKEGKTSFPEAEAMKILQAYNFPLLKSAVAK
                     SASEAKQILEKFACAVAMKIVSPDILHKSDVGGVMLNVTADNVEAKFQAMMTTVRKNK
                     PQAKLEGVLLMEMAPQGHETILGINKNSLGSMLMFGLGGIYVEVFKDVTFAFPPLSKL
                     EIEKMIGGLKSNSIFMGARGQAAIDQKSLIEAIARLAQLAGDFPQIKELDINPLLLTA
                     QGVKVLDARIVIE"
     gene            complement(5305..6057)
                     /gene="pflA"
                     /locus_tag="PLJ02_01895"
     CDS             complement(5305..6057)
                     /gene="pflA"
                     /locus_tag="PLJ02_01895"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007758999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HOI05200.1"
                     /translation="MVKTLKIHSIETFGVHDGPGIRLVVFVQGCPFRCLYCHNPDTQE
                     LETAKTQTKTIDEILALLEREKPYFGENGNRGGITVSGGEPTLQAKNMITLFRKAKER
                     GFHTCLDSCGGIVNKDVEELYRFTDLLLLDVKHIDPLWHRRLVGQDNAKVLENAKIRE
                     KSGREMWLRYVLVPGWTDQPEYLTAWAKYFAHFKTVSRVEILPYHELGKHKYQELGRE
                     YLLANVKPPSQEQIAPAKEIFTKYLGNKVVVN"
     gene            complement(6059..>6623)
                     /locus_tag="PLJ02_01900"
     CDS             complement(6059..>6623)
                     /locus_tag="PLJ02_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine radical domain-containing protein"
                     /protein_id="HOI05201.1"
                     /translation="NDDDRVDRIATDVVKRFNAELAKNHIYRDATPTLSVLTITSNVV
                     YGKKTGATPDGRKAGEPFAPGANPMHGRDHSGAIASLNSVAKIDYGDARDGISNTFSI
                     VPKALGSDRSQQIDNLVGMLDGYFNQKAHHLNVNVLNRETLEDAMEHPEKYPQLTIRV
                     SGYAVNFIKLTRALQEEVIARTFHETM"
BASE COUNT         2033 a         1354 c         1074 g         2162 t
ORIGIN      
        1 aattaataga aaacagggat aatttagcca cttcaattaa agcaaccgtt ttttcgcaat
       61 aaatttcttg atacttaatt aaggaattaa tctgctttgt taaatttaag taacgataaa
      121 aagcaaaaat aattagcaac attaaacata acagtaagat taaaattagt tgaatcaaaa
      181 tcaatattaa caacgcatgg ttgataattc gccaggaaaa taatttaaag atcagtaaaa
      241 gcattgcttt gcagtttctt ttttatgctt ttaaaaatct tatcctggcg accaatgtga
      301 ttgaaatgag cctgatgcaa atttaatgaa aaatcttctt taccgaaagc taacctccga
      361 tagatctttt gtattaagta gagaaaacca ttaattggcc agagaaaata atcgattaaa
      421 ttgtctttat cactggacaa atcattattg ggcagattaa ggtctagtaa ttcagcaaac
      481 tgccaatttt gtaaccagag cttcttttgg atagacgcct tatcgaatag aaagtccatt
      541 tgccttaact catatgcctg atagatagaa aaaccccttt gagctatatt taatgagctt
      601 tcatctaacc ataaattaaa acaccaagct tgtttatttt catttttagc atgggcatca
      661 atttgtggcc gttttcccag aattttggct accacaatta ccaccaaacg agtaagccac
      721 aaagtgtttt tcttaacgat aatacaaaaa tctaaatcat cggctaaact agcgttgttc
      781 atcgcacttg caccagtcaa accaagagct tggatactcg gaaaacgcaa caagtattga
      841 ctggcttgta gaataacagc gcgtctgtta taagcgatct tttccttatt taatctcaat
      901 ttaactaatt gttgtcgatt ggttaaaaaa taatagtcat catctgctac ttttaattta
      961 gcaatttttt tcgccttttc cagtttttct aaacttttgg taaatttctc ggaattttta
     1021 tcgattttaa accgatcact tttaagttta actccagtta aaaaatccca atcccacact
     1081 tttggtaaac gttggtaaat ttcagttttt tttagcgggt aattaaaata gtcactgtaa
     1141 acgagggtaa aaaaaactgc atggtctaaa tcattaaaat aaacagccac ctagttatca
     1201 taaactatcg agtaaaaatt cgctataata agagttatga aaatttattc ctggaacgtc
     1261 aatggtatcc gttctgtagc caaaaatggt tttgttgctt ggctcactaa ggaaaaacca
     1321 gacattttgt gtgttcaaga aaccaaagct catcccgatc aactcagttt tgatttaaaa
     1381 atgattgacc actatcgagt tgactttgct agtgcgcaca aagctggtta tagtggcgtg
     1441 gccacttata gtattcccga acctaacaaa gttcaaattg gtctgaatca acaacgtttt
     1501 gatcaagaag gtagagccat tgttagttac taccccaatt ttattttact aaatatttat
     1561 ttccccaatg gaaaagctag tcaagaacgc ctgcaatata aaatggcttt ttatcaatat
     1621 ttattagctt atttgccgga tttaaaaaaa gttaatcaaa atattatcat taccggtgat
     1681 gtcaatacgg cccatcagga aattgatctc gctagaccca aagctaatga acatatttct
     1741 ggttttttga aggaagaaag agcctggata gatcaattac taaattcagg tttcattgat
     1801 agttatcgct acttttatcc acagcaaaaa gatgcttaca cctggtggag tcaaagaaca
     1861 gcagctagaa aacgtaatgt tggttggaga attgattatt tcttcgttag tcagtcgctg
     1921 aaaaaaaatt tacaagcagc cacaattcat cgcgaagttc caggctcaga tcactgtcct
     1981 atttctatcg agctaaaatt ttaatccatt taaattgata aattacttag tgttactgat
     2041 aaattactca gtattgctaa taaattacaa agaaaagtat tcttttaact cctgccacaa
     2101 atcggcaatc ccttgtttat tatcgtcaac agaattgtct aaaataaaaa gtcgcccgta
     2161 ctgatgatcg ctgatcgctt gttcaataat ttttttcttt ttagcaaaat catattgact
     2221 ggctagtctt cttttaattt gctcttggct taaacctcgt ttaattaagc gttgggcttg
     2281 agtttgctcg ttaacatcta ataaaatcaa attaaagttg ctcaaatcca tcatttgcgt
     2341 ttcagccaac agggcggcgt ttaagattat taaaccttct ttatttttta gttctttgcg
     2401 tagtctcacc aaaattgggg tccgcataat cttgtttaat ttatttagct tttcactgtc
     2461 agcaaaaaca atttcaccta aagcttttcg atcgattctg ccatcttttt tggcaacttc
     2521 ttgaccaaaa tttttaataa tttcttgtct aactaattca tacttttctt cgcttaaatc
     2581 ttcttgaatt tgctgagcca ataaatcaag ttcaatatta tgagcagaaa tcttgtcttt
     2641 ttttgctagt tgcactagct gttcgccaac ataactcttc ccactgccaa tttcaccagt
     2701 caaaccaaca atatattgtt tggataactt tttttctaaa cattgtttaa cataaggact
     2761 aacgtagtct tgaattaagc cctgatcctt ttgaatttcc ttaactgcgc tggaactaat
     2821 atgtgacaat tcaggtttag taaataataa gaaggtatca atacccaaac gctgactttc
     2881 acccattaag tgtaagttct gctcatattc aaaatcttga ttggttctaa cacctttgac
     2941 aatcacgtcc acattatttt cataagcaaa atcaactagc agaccagtaa aagacattac
     3001 tttaacattt ttcagattga ctaaactacg tcgagccatt tcttctcttt cctcgagagt
     3061 aaacagataa ttttttaagc gattaacacc aatagccaca attaactgat caaaggcctt
     3121 ggcagctcta gtaataatat ctaaatgacc gtgggtaatt gggtcgccag aaagggcgta
     3181 taaagctctt gtcataattt aattattcaa taacaatgcg tgcatcaagc acttttactc
     3241 cttgagcagt taataataat ggattaatgt ctaattcttt gatttgcgga aaatcgccag
     3301 ctaactgagc caagcgggca atagcttcaa ttaaactttt ttgatcaatc gctgcttgac
     3361 ctctagctcc cataaaaata ctgttagatt ttaaaccgcc gatcattttt tcaatttcta
     3421 acttacttaa tggaggaaaa gcgaaagtta catctttaaa aacctcaaca taaatcccac
     3481 ctaaaccaaa cattagcata ctccccagcg agtttttatt aatacctaaa atagtttcat
     3541 gaccctgggg tgccatttcc attaataaca ccccctctaa tttggcttgt ggtttatttt
     3601 tacggacagt ggtcatcatt gcttgaaatt ttgcttcaac attgtctgct gtcacattta
     3661 gcatcactcc gcccacatca cttttgtgta aaatgtctgg agaaacgatt ttcatcgcta
     3721 ccgcacaagc aaatttttct agaatttgtt tggcttcact cgctgattta gccacagcac
     3781 ttttcagtaa gggaaaatta taagcttgga gaattttcat cgcttcagct tctgggaaac
     3841 tagttttccc ttcttttttg gctttcttaa atatttgagc aacccgttct cgatcaacgt
     3901 cattaaaaac taatggttta tcttctttaa ttttagtcca agccacaaat ttagctaaag
     3961 cgcccaaacc taaagcagct gattcaggaa aagtggtatt agcaatttta ttatctttta
     4021 aaattttcac tccatctgca actaaatctc gacccattaa ggaaacagca atgactttat
     4081 ctgttttttt tcttaaattc actatcgcct gggctgtttt gactggctca gtcatggttt
     4141 gtggggtcag caataccaaa atcgcgtcag tttgccgatc gtcaccaatt actgctaggg
     4201 ttttttcata gacctcacca accgcatcac ctaaaacatc tacgggatta tgagtattgg
     4261 ctgcggctgg caaaaaactc tttaattttt tgatcgtttg ctcttccaat ttagccatct
     4321 ccaaaccatt ttctatgacc gcatcagtgg ttaaaactcc gggaccacca gcattagtga
     4381 taatcgtcac cgttttaatt tccttaagag gattattact aaaaattgag gctaaatcaa
     4441 ataattcact gaccgattgt gctcggataa tcccagcctg attaaacagt gcttgataag
     4501 cattatcacc accagaaagt gaaccggtgt gagaagcaat ggctccagct ccagcggctg
     4561 ttttaccaga tttaagcaca atcaccggtt ttggctcttt gccgcgattt aactctttta
     4621 gcgtttgaat gatggcggtt gcattatcta gtgcctcagc atataaacaa atcactttgg
     4681 tttgcggatc ctcatgtaaa tatttaatta atgctaattc gtctagagcg gctttattgc
     4741 caatactaat aaatttggag aaacctaaac ctaattcttg agcgtagtcc aagactgctg
     4801 tacaaagtgc tccgctttga gacataaaag ccacgttgcc tacttctggc ataatggcag
     4861 caaatgaagc attcatttta tgctcagcat taatcacccc taaacaattt ggcccaacca
     4921 gaatgatccc gtgacgtcga caagtagcag ccagttcctg ctctaatgcc aaattaccag
     4981 cttctttaaa accggctgaa atcacaatgg ctgcctgagc ccctttgcta gctgcttcgt
     5041 caatagctga ggcgacaaac ttggctggga tggccaccac taccaaatca attttttgat
     5101 ccaagtcctt gatatcgtga tagactttta aaccataaag ttcatcaatt ttgggattaa
     5161 ttggataaat ttttccagca taaccctgat taactaagtt ttttaccaca tcattaccca
     5221 cagtcttttc tcgactagaa gcgccaataa tggcaattgc ctgaggttga aaaagagctt
     5281 gaaaattcat atctgctttc tattttagtt gactaccacc ttattaccta aatatttagt
     5341 aaaaatttct ttggccggag ctatttgttc ctgactaggc ggtttaacat tggccaacag
     5401 gtattctcta cctaattctt gatacttatg ctttcctaat tcgtgatagg gtaaaatttc
     5461 caccctactg actgttttaa aatgcgcaaa atattttgcc caagctgtta aatattcggg
     5521 ttggtctgtc cagccaggca ctagaacata acgcaaccac atttcccgac cacttttctc
     5581 cctgatttta gcattttcca aaacttttgc attatcctgt ccaactaagc ggcggtgcca
     5641 tagcggatca atgtgcttaa catcaagtag aagcaaatcg gtgaaacgat ataactcctc
     5701 aacatcctta ttgactatac ctccacagct gtctaaacaa gtgtgaaacc ctcgctcttt
     5761 agcctttcta aataaggtta tcatgttttt agcttgcagg gttggttcgc cccccgaaac
     5821 agtgattccg ccccgattgc cattttcacc aaaatagggt ttttctctct ctaaaagcgc
     5881 aagaatttca tcaatcgttt tagtttgagt tttcgcagtt tctaactcct gagtatctgg
     5941 attgtgacaa tataaacaac gaaaaggaca accttggaca aaaacgacta aacgaatgcc
     6001 tggcccatca tgaaccccaa aagtttcaat tgagtgaatc tttagagttt taaccatctc
     6061 acattgtctc gtggaaagtg cgcgcaatca cctcttcttg tagggctcta gtcaatttga
     6121 tgaaatttac cgcataacca gaaacgcgaa tggttaattg tggatatttc tcaggatgtt
     6181 ccatagcatc ctcgagtgtt tctcgattca acacattgac attaagatga tgagcttttt
     6241 gattaaaata accatctaac atcccgacta aattatcaat ttgttgagag cgatcactgc
     6301 ccaaagcttt tggtacaatt gaaaaggtgt tagagattcc atctcgagca tcaccataat
     6361 caattttggc cacactattt agagaagcaa tggcgccaga atgatcacgt ccatgcattg
     6421 ggttagcccc aggagcaaaa ggttctccag cttttctgcc atctggtgta gctccagttt
     6481 ttttgccata aaccacattg gaagtaatgg ttaacaccga taaagttggc gtggcgtctc
     6541 gataaatatg attttttgct aattctgcat taaaacgctt aactacgtct gtcgctattc
     6601 tatcaactcg atcatcgtca ttg
//
LOCUS       DAOSEW010000034         6604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_10150_length_6604_cov_4.373645, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000034 DAOSEW010000000
VERSION     DAOSEW010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 6604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6604
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10150_length_6604_cov_4.373645"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..1704
                     /locus_tag="PLJ02_01905"
     CDS             70..1704
                     /locus_tag="PLJ02_01905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOI05202.1"
                     /translation="MLIKLFKKITQHQYFSAVVLALIFLIAAYLRLVNLNWDQSLALH
                     PDERNIALAVSRLNWPEQTDPAFYAYNGFPLFLADLSSQLMSNLTNDVSWQNDLGKIN
                     LITRTYSAIFSLISVIFFYLIAKKIFSKNTVLIVTWLAATTVTLIQHAHFGVTESLLV
                     LELLMLTWLSICFIQEKKQRFLLAQALVLGLSLATKTSALPFAIIPALSIWLVNKFNF
                     KALRQMSAVVVVSFLVFYLTSPHTFHYFQQFLSTMRYEQAIASGQQKIFYTMQFIGTI
                     PYLFQLKTLLWQTSPLFLLIAGGGLYWFFKNFKKYRVLWPLILFSIIYFLYIGSWYAK
                     FNRYLMPVIPALILLAGVAIEQFPQKRPQKILISLMVITHGLWALAFIHIYQTEDVRL
                     TASHWIEDNIASDSVLLHEERDVRLPVVFSQGKNYQYSLLELYPVDSQEKINNLSQQL
                     SSGDYLLIASQRLYRTIPKSADHPDTKNYYQLLFAGELGYQKITEFSSYPKLLGLEIN
                     DERAEETFTVFDHPHIFIFKNVDHLSQEELFKKIRP"
     gene            1900..2637
                     /locus_tag="PLJ02_01910"
     CDS             1900..2637
                     /locus_tag="PLJ02_01910"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOI05203.1"
                     /translation="MKKDTLIFIVVSLILGAGLIIGLNKLRPASGALVEDNTVDELQN
                     INTNEKENLDQLSSNPTIEKDYSMPATFSANQTLTKPAMMLDLNKTYLVTLNTNQGPI
                     TIELDTQNTPITSNNFVYLAEKGFYNNTVFHRVLDGFMIQGGDPRGDGTGGPGYHFDD
                     EDFVGEYTRGTVAMANSGPNTNGSQFFIMHQDYQLPKNYVIFGHVVEGLEVVDAIATA
                     PVEMSLSGEKSRPINPVVIKSAIVTSN"
     gene            2637..2975
                     /locus_tag="PLJ02_01915"
     CDS             2637..2975
                     /locus_tag="PLJ02_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05204.1"
                     /translation="MPKNKEEQSAYQPSILDWQENQDPEKKEAYYSFPEGTPMWRDGH
                     KGMVTAVTSFVAKRVEGKIAGYAAFELVQNVKVFYQRGWEPKSYFYLKTGEKMGISTS
                     SKGFKPTKFK"
     gene            complement(2968..3825)
                     /locus_tag="PLJ02_01920"
     CDS             complement(2968..3825)
                     /locus_tag="PLJ02_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013756245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOI05205.1"
                     /translation="MSIQITDLQSPISPTWCPGCGNFSILTALKKALIELNLEAKDVV
                     IVFGIGCSGNMADFIKTNGFHALHGRAIANAIGIKLANHRLKVIVTGGDGDLYGEGFN
                     HLLAAARGNHDISVFVHNNERYSLTTGQTSPASKKGTVGKSTPQGVIEEAINPLALAI
                     SANASFVARGFAGQSQELVELMKQAIMHEGFALVDILQPCPSFNKTQDNQWYIEHTQK
                     LKADYDLSDREQALKQVLKKEPIELGVFYQDQGRLAYHQELQQLTDKSLIEQFPDHLD
                     LQLAIEQFI"
     gene            complement(3831..5621)
                     /locus_tag="PLJ02_01925"
     CDS             complement(3831..5621)
                     /locus_tag="PLJ02_01925"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOI05206.1"
                     /translation="MEKIQLNWKIAGKAGDGVMLAAKFFAKLCQHHGLQVFNYYEYPS
                     LIKGGHQTGQVYASSESASCQRRHLDLLVLFHPKFLAFHLDALDEKSVVLINAKSFDS
                     SQYPQLKARVITIPFSQIAKQEVGDALMANMMMIAASAKFFDFKSDQLLELVKKEFAD
                     NRRIIEQNQQAINLAYQELEKSIQEAQLDCSDQTLEVKAATDKNLLLTGNEAVGLGAI
                     AAGLQFYSAYPMTPATGLLHYLAAQQENYPLVVKHTEDEIGAINQALGAAFAGVRAMT
                     GTSGGGYALMVEATSLAGVAEIPLVILEAMRPGPATGLPTWSGQADLNFIVHAGHGDF
                     PKVVFTPGSVLEHYQLSKLAFYIAEKYQLPVFILSDKYILESQQTMPQPDLAIDLERF
                     SLLANQQSTGEKYLRYQDTDSGISPRAIPGLDKVVYLANSYEHDQEGFATEESLVTKR
                     AMDKRLRKMETMLAELPKPQVFGAPVTAADKVLISFGSTINVLQELLNLLVAAKINQP
                     AIIHLPCVAPFLSKDFEQLFDWQKINEGKQAVYTVEGTATAQLAQLLRANSQLKNIKS
                     ILRYDGRPFYAEDLLEWFQTQKINEVYQIK"
     gene            5726..6175
                     /locus_tag="PLJ02_01930"
     CDS             5726..6175
                     /locus_tag="PLJ02_01930"
                     /inference="COORDINATES: protein motif:HMM:NF012481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOI05207.1"
                     /translation="MKNLLIYGTLTGNTEAVAKQIKKIVEEAGKELEIKNINQTNVED
                     IAQVENLLIVASSTWDDGLPCSDMADFLEENEANLPDLTGKKLAFFGCGDSNYIQFCH
                     AVDILEEKFTAKGGQKIGDSLKIDGYIEDQSNQENIKKFAEQLIQSL"
     gene            complement(6210..6548)
                     /locus_tag="PLJ02_01935"
     CDS             complement(6210..6548)
                     /locus_tag="PLJ02_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05208.1"
                     /translation="MANKKKGLLPLVIGAIAGAVAVILSDEKNRAKAKKTIDEAKKDP
                     QAFSQKTVKKAEQTAKEIAAKAKAAAAKAKMAAEKEAKIVAKKAKTAGKKAIEQHQTQ
                     TTSKVKGKAK"
BASE COUNT         1993 a         1331 c         1188 g         2092 t
ORIGIN      
        1 catcctgtca aaaaggtatt tctagagtct cttcctgtca aaaaggcaat tttattgtaa
       61 cattaccttg tgttaattaa attatttaag aaaattacac aacaccaata tttttctgcc
      121 gtagttttag cattaatttt cctaatcgcc gcctatttgc gtttggtcaa cctcaattgg
      181 gatcaaagtc tagcgctaca ccccgatgaa cgcaatattg ccctggccgt ttcgagatta
      241 aattggcccg aacaaactga tcccgcattt tacgcctata acggttttcc cttgtttctt
      301 gccgatctta gttcacagct aatgagtaat ttaacaaatg atgttagttg gcaaaatgat
      361 ctaggaaaaa tcaacctgat cactcgaacc tattcagcta tattttcttt gatcagcgtc
      421 attttcttct atctcatcgc caaaaaaata ttttctaaaa atactgttct cattgttact
      481 tggctagcag ctactactgt tactttgatt caacatgctc atttcggagt cactgaaagt
      541 ttgttggtgc ttgaattatt aatgctaact tggctgtcga tttgtttcat tcaagagaaa
      601 aaacaacgat ttttgctagc ccaagcgctt gttctgggct tgagtttggc cacgaaaact
      661 tctgcactgc cttttgccat tattcctgct ctttccatat ggttagttaa taaatttaat
      721 tttaaagctc ttcgacaaat gagtgctgtg gtggtggtga gttttttagt gttttatctg
      781 acttctcccc atacttttca ctattttcag caatttttaa gcaccatgcg ctatgagcag
      841 gcgattgcca gtggtcaaca aaagattttt tacaccatgc aatttattgg caccattcct
      901 tatttatttc agctaaaaac tctgctttgg caaaccagtc ccttgttttt attaatcgct
      961 ggcgggggtt tgtattggtt ttttaaaaac tttaaaaaat atcgagttct ttggccactc
     1021 atcctgtttt caataattta ttttctttac attggtagtt ggtatgccaa atttaatcgc
     1081 tatttgatgc cagttattcc tgctttaatc ttattagctg gcgtggcgat tgagcaattc
     1141 ccccaaaaac gtccccaaaa aattttaatt agtttaatgg tgatcacgca cggtctctgg
     1201 gcactcgctt ttatccatat ttatcagaca gaggatgtta gactaactgc ctcacactgg
     1261 atagaagaca atattgctag tgatagtgtc cttcttcatg aagaaagaga cgtacgactg
     1321 ccagtggttt ttagtcaagg caaaaattac cagtactcac tattagagct ttaccccgtt
     1381 gatagtcagg aaaaaattaa caatttgagt caacaattaa gttctggaga ttatttgctg
     1441 atcgctagcc aacgcctgta tcgtactatt cctaaaagtg ctgatcaccc tgataccaaa
     1501 aattattatc aattattatt cgctggagaa cttggttatc aaaaaataac agagtttagt
     1561 tcttatccca aattacttgg actagaaatt aacgatgaaa gggctgagga aacttttacc
     1621 gtctttgatc atccacacat ttttatcttt aaaaacgtcg accaccttag ccaagaagaa
     1681 ttgttcaaaa aaattagacc ttagttgaaa tagatacgtt attcatgagt gttgtcctag
     1741 tttttatatc aaaatatatc aatcagtata taaaatatac aagatatatc aatcaaaaac
     1801 ttttttaacc cttattagca gacaactgat ttaactgcta aaatatttta ttacaaatca
     1861 tagacattgt gatatactaa taaatctcga ttacaaacta tgaaaaaaga tacattaatt
     1921 ttcattgtgg ttagcttaat tttgggtgcc ggcttgatta ttggtttaaa caaattgcga
     1981 ccagctagtg gagctttagt tgaagataac accgtagacg aattgcaaaa cattaatact
     2041 aacgaaaaag aaaatcttga tcaattatcc agtaacccaa caatagaaaa ggattatagt
     2101 atgcctgcta cattttctgc caatcaaact ttaactaaac cagcaatgat gcttgatcta
     2161 aacaaaacct accttgtcac attaaatacc aatcaaggcc cgattactat tgaattagac
     2221 acccaaaaca cgccaatcac cagcaataac tttgtttatt tagctgaaaa agggttttat
     2281 aacaataccg tctttcatcg agtgctagat ggttttatga tccagggtgg cgatccccgt
     2341 ggcgatggca ctggcggccc cggctatcat ttcgacgatg aagattttgt tggcgaatac
     2401 actcgtggca cagtagccat ggctaactca gggccaaaca ccaatggtag tcaatttttt
     2461 attatgcatc aggattatca actaccaaag aattatgtca tttttggtca cgtggttgaa
     2521 ggcctcgaag tagtagatgc tattgctact gcccctgtag aaatgagtct ttctggcgaa
     2581 aaatctcgac ctatcaatcc agttgtgata aaatcagcta tagtaacaag caattaatgc
     2641 caaaaaataa agaagagcaa tccgcctatc aaccttcaat actagattgg caagaaaatc
     2701 aagatccaga aaaaaaagaa gcttattatt cttttcccga aggtactccc atgtggcgag
     2761 atggccataa agggatggtg actgccgtga ctagttttgt ggctaaaagg gtagagggta
     2821 aaattgccgg ttatgctgct tttgagttag tgcaaaatgt caaagttttc tatcaaagag
     2881 gttgggaacc caaaagttac ttttacctga aaaccggcga aaaaatgggt atttccacat
     2941 caagtaaggg ttttaaacca accaaattta aataaattgc tcaatagcca gttgtaaatc
     3001 taggtggtca ggaaattgct ctattaaact tttatctgtc agttgttgca actcttgatg
     3061 ataggccagt cgcccctgat cttgataaaa cacaccaagc tcaatgggtt ctttttttaa
     3121 cacttgttta agagcttgct cacggtcact cagatcgtaa tctgctttta acttttgggt
     3181 gtgttctatg taccattggt tgtcttgggt tttattaaaa ctgggacaag gctgtaagat
     3241 atccaccaaa gcaaaaccct catgcataat ggcttgtttc attaactcta ctaactcttg
     3301 tgattgacca gcaaagcctc gggccacaaa gctcgcatta gcactaatag ccaacgctaa
     3361 aggattgatc gcttcttcaa ttactccttg aggagttgat ttgcccactg tccctttttt
     3421 gctagctggg gaagtttgcc cagtggttaa actataacgc tcattattat gaacaaaaac
     3481 actaatatca tgattacctc gagccgccgc taacaaatga ttaaaacctt caccatataa
     3541 atcgccatcg ccaccggtaa ctatgacttt taaccgatga ttggccaatt taataccaat
     3601 agcgttggca atagctcgac catggagcgc gtgaaaacca ttagttttga taaaatctgc
     3661 catattccca ctgcaaccaa tcccaaagac aatgacaaca tctttggctt ctaaatttaa
     3721 ttcaattaaa gcctttttca gggcagttaa aatgctaaaa ttgccacaac caggacacca
     3781 ggtaggtgaa ataggtgatt gtagatcggt gatttggata gacatatgat ttacttaatt
     3841 tgataaactt cattaatttt ttgagtctgg aaccactcta atagatcctc ggcatagaat
     3901 ggtcgaccgt catagcgcaa aatgctcttg atatttttta attgactatt agctctcagt
     3961 aattgggcta attgagccgt cgctgtccct tcaacggtat aaactgcttg tttaccctca
     4021 ttaatttttt gccaatcaaa aagttgctca aaatctttgc ttaaaaatgg agcaacacaa
     4081 ggaaggtgaa taatagctgg ctgattaatt tttgctgcta ctaataaatt taataattct
     4141 tgtaagacgt taatcgttga cccaaaacta atcaaaactt tatctgcggc tgttaccggt
     4201 gctccgaaaa cttgtggttt aggcaattct gccaacattg tttccatttt gcgcaaacgc
     4261 ttgtccatcg ctcgtttggt gactagcgac tcttcggtag caaaaccttc ttggtcgtgt
     4321 tcatacgaat tggctaggta cactactttg tctaaaccgg ggattgctct gggcgaaata
     4381 ccactatcag tgtcttgata tcgcaagtat ttttcgccag ttgactgctg gttagctaaa
     4441 agagaaaagc gctcaagatc aatagctaag tctggctgag gcatggtttg ttgagattct
     4501 aaaatatatt tatccgataa aataaaaact ggtaactgat acttttccgc tatataaaaa
     4561 gctaatttac ttagttgata atgctctaaa actgatcctg gggtaaaaac gactttggga
     4621 aaatccccgt gccccgcgtg gacgataaaa tttagatccg cttgtccact ccaggttggt
     4681 aaaccggtcg ctggtccagg tcgcatcgcc tctaaaatca ctaaaggtat ttccgcaact
     4741 ccagccaaag atgtggcctc aaccattagc gcataacccc ctccagatgt accagtcatt
     4801 gcccgcacac cagcgaaagc agcgcctaaa gcttgattaa tcgcaccaat ttcatcttca
     4861 gtgtgtttca ccactagggg ataattttcc tgttgagccg ctaaataatg caacaaacca
     4921 gtggcaggag tcattgggta agcgctatag aactgcaagc cagccgcaat tgcccccaaa
     4981 ccgactgcct cattaccagt taacaaaaga tttttatccg ttgctgcttt cacttccagt
     5041 gtttgatctg agcaatcgag ctgagcctct tggattgatt tttccaactc ttggtaagcc
     5101 aaattaatgg cttgttggtt ttgctcaata atacgtctat tgtcggcgaa ttcttttttc
     5161 actagttcga gcaactggtc gctcttaaaa tcaaaaaatt tagcactagc agcaatcatc
     5221 atcatattgg ccatcagcgc atcaccgact tcctgcttag ctatttgact gaaaggaata
     5281 gtaataactc tggccttcaa ttgaggatac tggctactat caaaactttt agcattaatt
     5341 aacactaccg atttttcgtc cagagcgtct aagtgaaaag ctaagaattt tggatgaaag
     5401 agcactaaaa gatctaaatg acgacgctga caagacgcgc tttcactact ggcataaact
     5461 tgaccagttt ggtggccacc cttgattaaa gaagggtatt cataataatt aaaaacttgt
     5521 aagccgtgat gctgacaaag tttggcgaaa aatttggctg ctagcatcac cccatcacca
     5581 gccttgcctg caattttcca atttaactga attttttcca tacatccgca ttgtagaagt
     5641 aatagtaaaa ttggtcaatc aaagattaaa aataatctgt taaaatgatg ttgtttgact
     5701 agtagcaact gatagaaagg tcacaatgaa aaatttatta atttacggga ctctgactgg
     5761 caacactgaa gccgtcgcca aacaaatcaa aaaaatagtt gaagaagctg gcaaagaatt
     5821 agaaatcaaa aacatcaacc aaaccaacgt tgaagacatc gctcaagtag aaaatttatt
     5881 aattgttgct tcctcaacct gggatgatgg tttaccgtgt agtgatatgg ccgatttttt
     5941 ggaagaaaat gaagccaatc ttccagattt gactggtaaa aaactggctt tctttggttg
     6001 cggtgatagc aattatatcc aattttgcca tgcagtggat attttggaag aaaaattcac
     6061 agctaaaggt ggacaaaaaa ttggcgacag tttaaaaatt gatggttaca ttgaagacca
     6121 aagcaaccaa gaaaatatca aaaaatttgc cgaacaacta atccaatcac tatagcttaa
     6181 aaacacgctg tttaaactta atcgatggac tatttagctt ttcccttaac cttggaggtg
     6241 gtttgagttt ggtgttgctc gatcgctttt ttgccggctg ttttcgcctt tttagccact
     6301 atcttcgcct ctttttccgc tgccattttt gcctttgcag cggctgcttt agcttttgct
     6361 gctatttctt tagcagtttg ttcagctttt tttaccgttt tttgagaaaa agcttgagga
     6421 tctttcttgg cttcatcaat cgttttttta gctttagctc gatttttctc atctgagaga
     6481 atcacagcta ccgctccagc gattgctcca ataactaatg gaagtaaacc ctttttttta
     6541 tttgccataa ttgttccttt ttttactttt aattaattag tttagcttaa ctctttttca
     6601 acaa
//
LOCUS       DAOSEW010000035         6579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_10207_length_6579_cov_3.983752, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000035 DAOSEW010000000
VERSION     DAOSEW010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 6579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6579
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10207_length_6579_cov_3.983752"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PLJ02_01940"
     CDS             <1..384
                     /locus_tag="PLJ02_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05209.1"
                     /translation="EDFVERSQKYTSLQAKERKNLKTISTEDLIQAFNSEFLRRFFLK
                     KAYQDGMHGLALSLLQSNYELLIKLKQWQAQDFPEEQLNNQQLIKLLKTQRVEFDYWL
                     TDLEISEAGWGRKLFLKLKRKIANR"
     gene            381..1304
                     /locus_tag="PLJ02_01945"
     CDS             381..1304
                     /locus_tag="PLJ02_01945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOI05210.1"
                     /translation="MKNHISIIIVNYNTPDETKDCLRSLDNLETWGFDYQVIVVDNGS
                     KVPLKLSSNFLRHHPRVELITSASNLGFTGGNNLGIKHAANTYDSDYFLLLNSDTVVS
                     NNFLKELYLLAKNDPKIGLISPKIYFHKGYEYHQNSYQPDQLGQVIWYAGGKIDWSNL
                     ISFHIGVDEVDRGQFDEPRETDFATGCCLLITREVVERVGTLDNRFFLYSEDVDYSLR
                     VKRAGLKVYYCPSALIYHKIASSTGGVGSQLQQYYQTRNRLLLSFKHGWRRRRLTTVH
                     LALRLLINGSASERKAVLDLIKSNLGKQSYV"
     gene            1297..2133
                     /locus_tag="PLJ02_01950"
     CDS             1297..2133
                     /locus_tag="PLJ02_01950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOI05211.1"
                     /translation="MSRAKITAVIIAKNEEAMLPACLETLAWCDEILLIDCASTDKTT
                     QVAEKYGARVIGFDHPSFAKIREKGLESVTTDYLFYVDADERVTPALAKEILVHLENQ
                     DCQVMNINRENICYGRKFHYGNWQNDQVIRVFHKDSLKGWQGEIHESPIYSGHVCQLS
                     HKLIHLTHRSTQENLIKSANWTIKEASLLAASEQTKPVTFFTILRKGFMEFYRRAFKY
                     QGRKDGLAGLIEALVQGINRMLVYIQVWELQQKPSLADRYHKQEMQIKKLWKTEGASL
                     KL"
     gene            2135..3250
                     /locus_tag="PLJ02_01955"
     CDS             2135..3250
                     /locus_tag="PLJ02_01955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOI05212.1"
                     /translation="MKIVFYSPYFPKHFGGGEKYLLDCITALLERGRDEVFLALPTQE
                     FLTQEQIAIIRQKYQQFCAYDLNKLNIIASPLGTEASFFTKLSWTKKFDVLYHLTDGS
                     LFFSLAKKNILHIQVPLPITKKSLVEKLKLKNYHIKNTNSYFTKNYVEKHWPVTIDYV
                     HQPLVNLAEFPVQKNPLAKKEKIILNVGRFFRQLHSKRQDVLVSLFARLIEAEPQLMA
                     GWQLHLVGSVEDQDYLAEIKTMAKNLPIQIHEQVDRGALIKLYQSASLYWQATGYGLD
                     ENTHPSAMEHFGIAPIEAMAAYCVPLLIAKGGHKEVLGSEFSDYLWESDEDCLNQTIA
                     LIKNEPERARVARAMRLRAEDFGAERFNQLLYKMIDD"
     gene            3260..5680
                     /locus_tag="PLJ02_01960"
     CDS             3260..5680
                     /locus_tag="PLJ02_01960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOI05213.1"
                     /translation="MKKSKQRPAVSVLIPSYLGQALLAQHLDQVLAIMRAGDQLIIID
                     DASPDEGATKNYLIKKFNLAPVTQDQWPAQLFQGQWQGIEIVVAINEHNLRFGATVNF
                     GFQLAKRSLVLLLNNDVVPQADLLDHLIPHFQHQKTFAVTCLEKNGKDKDTWAGKNKL
                     WFEQGMFQHSKADDLLSGPTAWASGGSSLIDRDKFLAIGGFDQRFKPAYWEDIDLSFQ
                     ARKRGWQVLFEAKAVVEHHHESTHQNVFGQAKIEQISRRNGKKFTKKNADFWQRMAYY
                     LWRPYWLIQEWRQPAPKQHLRHYLWLAAILLVAIGLRFYQLGRVPAGMAVDEVAIGYN
                     GFAIWQTHRDEWLEFLPISFRSYGDYKAPLAIYINGLSTALFGLNLWAVRLPFAVFSI
                     LAIVGFYLLAKEVLAQNQLDKNLALWAALFLTLSPWHIHFSRLGFEAGMALSLLIWAV
                     YFWYRYWRQQRLGQLLLSAFLACLTLYTYHSSKVTTPLLFVLIFLAQRQRLTIKPKHL
                     LAALALTLFLISPLAYDAIYGEGLTRAGSSIILSELSLAEKCKVLLTNALSYFSWDFL
                     ARGNVNGELRHGDGQFGVLSYPALALIIGYVLSLFSKKIRFNDHSRPILVLIGLWLVA
                     GYLPAIIGDQPYHSNRALLALPAWIFLEVLACERLSQFFQDHRWGLHYPLLVAYLLFT
                     LIYQRHYYQDYNRLSADAFNEGYLETVEYLKQLDKNGVEKILFTNDYQHAYIYVLFGN
                     QVSPIAYHGGILVNYEFSEDIDFQDLNREKTIVVASKDDEMMDYQPDQIIYGSDGSQR
                     FRIYLPKE"
     gene            5770..>6579
                     /gene="argS"
                     /locus_tag="PLJ02_01965"
     CDS             5770..>6579
                     /gene="argS"
                     /locus_tag="PLJ02_01965"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOI05214.1"
                     /translation="MQIEEQLRTAIRASLPEKLAKLINPEEIVLEQPKDSNFGDFSTN
                     LAMLKYDLVADDFASPRELAALIVKNFQLQQPAITASVAGFGFINFKLSDSIFWQIFA
                     DLPQSEAELIPATKHSQKVIVEYSSPNIAKPFNIGHLRSTIIGDAVANLYQAVGYQVF
                     RDNHLGDWGTQFGKQIVAIKKWGDEEAIASSASPVAELVKLYVKFHQEAEQDESLNDE
                     ARWWFKKLEAGDQEARRLWQRCIDWSLSEFNKIYQRLGVKFTENDGRGYGES"
BASE COUNT         1888 a         1167 c         1508 g         2016 t
ORIGIN      
        1 gaagattttg tagagcggag tcaaaaatat acttccctcc aagccaaaga gagaaaaaat
       61 ctcaaaacca ttagcactga agatttaatc caagctttca atagtgaatt tttgcgtcgt
      121 ttcttcttga agaaagctta tcaagatggg atgcatggtt tagcgttgag tttgctccag
      181 agcaactatg aattattaat taaattaaaa cagtggcagg ctcaagattt tcctgaagag
      241 caacttaaca atcagcaact gattaaatta ctgaaaacgc aaagagttga gtttgattat
      301 tggctgactg acttagaaat tagtgaagct ggttgggggc gaaagctgtt cttaaaactg
      361 aaaagaaaaa tagcaaaccg atgaaaaatc acattagtat tattattgtt aactacaata
      421 ctcctgatga aaccaaagac tgtttacggt ctttggataa tttagaaact tggggttttg
      481 attatcaggt gatcgtggtt gataacggtt ccaaagtacc acttaaactg agtagcaact
      541 ttttgcgtca tcatccacga gtggaattaa ttactagtgc gagcaatctc ggttttactg
      601 gcggtaacaa tctgggtatt aagcatgcgg ctaataccta tgatagtgat tatttcttgt
      661 tgctcaatag tgataccgtg gtcagcaata attttttaaa agagttgtat ttactagcca
      721 aaaatgatcc aaaaattggt ttaatttccc ccaagattta tttccacaaa ggttatgaat
      781 atcaccaaaa cagttatcag cccgatcagc tggggcaagt gatttggtat gcgggcggga
      841 aaattgattg gtcaaattta attagttttc acattggggt cgatgaggta gatcggggcc
      901 aatttgacga gcctcgggaa acagattttg ctactggttg ttgtttatta attacccgag
      961 aggtggtgga gcgggttggc actttagaca atcgtttttt tctctacagt gaagatgttg
     1021 attacagtct gcgagtgaag cgggctggtt taaaagttta ttattgtccg tcagctttga
     1081 tttatcataa aattgccagc tcaactggtg gggtgggttc tcaactgcaa caatattacc
     1141 aaacccgtaa tcggttattg ctgagtttta aacatggttg gcgacggcgt agattaacta
     1201 ctgttcattt agctttacgc ttgttgatca atggttcggc tagtgagcgc aaagccgtgc
     1261 tagatttgat taaaagtaat ttgggtaaac aaagttatgt ctagagcaaa aatcactgcg
     1321 gttattatcg ctaagaatga agaggcgatg ctaccagctt gtttagaaac cctagcttgg
     1381 tgtgatgaga tcctgttaat tgattgtgct tctacagaca aaactaccca agttgccgaa
     1441 aaatatgggg cgcgggtgat tggttttgat catccgtcct ttgctaaaat tcgcgaaaaa
     1501 ggtttggaga gtgtgaccac tgattatctt ttttatgttg atgctgacga gcgggtgacg
     1561 ccagctttgg ccaaagaaat tctagtgcat ttagaaaatc aagattgcca agtgatgaat
     1621 attaatcgag agaatatttg ttatggtcgt aaatttcatt atggtaattg gcaaaacgat
     1681 caggtgattc gtgtttttca caaagacagt cttaaaggtt ggcagggaga gattcatgaa
     1741 agtccaattt attcgggtca tgtttgtcaa ttaagtcaca aactaattca tctcacccat
     1801 cgctcaactc aggaaaacct gattaaaagt gccaattgga ctattaaaga agccagttta
     1861 ttagctgcca gtgaacaaac caaaccagtc acctttttca ccattttacg taaaggtttt
     1921 atggagtttt atcgccgagc ctttaaatat cagggaagga aagatgggtt ggcgggttta
     1981 attgaagcgc tggtgcaggg gattaatcgg atgttggtct atattcaagt ttgggaatta
     2041 caacaaaaac ccagcctagc tgatcgctat cacaaacaag aaatgcagat caaaaaatta
     2101 tggaaaactg aaggtgccag tttaaaacta taacatgaaa attgtttttt attctcctta
     2161 ttttccgaaa cattttggtg gaggagagaa atatttacta gattgcatca ctgctctact
     2221 cgagcggggt agagatgaag tctttttggc cctgccaact caagaatttt taactcagga
     2281 gcaaatagcg attattcgtc aaaagtatca acaattttgt gcttatgatt taaacaaatt
     2341 aaacattatt gcctcgccac tgggcactga ggctagtttt tttactaagt tgtcctggac
     2401 aaaaaaattc gatgttttgt atcatttaac tgatggttcg ctgttttttt ctttagctaa
     2461 gaaaaatatt ttacacattc aagtgccgtt accgatcact aaaaaatctt tagtggaaaa
     2521 attaaaatta aaaaattacc acattaaaaa taccaattcc tatttcacta aaaattatgt
     2581 cgaaaagcat tggcctgtca ccattgatta cgtccatcag cctctggtca atttggctga
     2641 gtttccggtt caaaaaaacc cactagccaa aaaagagaaa attattctca atgtcggtcg
     2701 tttttttcgc caactgcata gcaagcgtca agatgtttta gtcagcttat ttgcccgcct
     2761 aatcgaagct gaaccacagc tgatggctgg ttggcagtta catttagttg gctcagttga
     2821 agatcaagat tatttggctg aaattaaaac tatggccaaa aatctaccga ttcaaatcca
     2881 cgagcaagtc gatcgtggtg ccttaattaa actctatcaa tcagcgagtc tgtattggca
     2941 agcaactggt tatggtctgg atgaaaacac ccaccctagt gcaatggaac atttcgggat
     3001 tgcgccgatt gaagcgatgg ccgcttactg tgtgccctta ttaattgcca aaggtggtca
     3061 taaagaagtt ttaggttccg agtttagtga ttatttgtgg gagagtgatg aagattgttt
     3121 aaatcaaacc atagctttga ttaaaaatga gccagagcgg gcgcgagtgg ctcgggctat
     3181 gaggctgagg gcagaagatt ttggggctga acgctttaat caattattgt ataaaatgat
     3241 tgatgattag gtttttaaca tgaaaaaaag caagcagcga ccagcagtga gcgtgttaat
     3301 ccccagttat ctggggcaag cgcttttggc tcagcacctc gatcaggtgt tggcgatcat
     3361 gcgagctggt gatcaattaa ttattattga tgatgctagc cccgatgagg gtgcaaccaa
     3421 aaattaccta attaaaaaat tcaatttagc gccagtgacc caagaccagt ggccagctca
     3481 gctttttcaa ggccagtggc agggcattga gatcgtggta gcgatcaatg aacataacct
     3541 gcgttttggg gcgactgtta attttggttt tcagttggct aagcggtcac tagtgttgtt
     3601 attaaataat gatgtggtgc ctcaagcaga tcttttagac cacctcatcc cccactttca
     3661 gcaccaaaaa acttttgccg tgacttgttt agaaaaaaat ggcaaggaca aagatacttg
     3721 ggcaggtaaa aataaactct ggtttgaaca aggaatgttt cagcatagta aggcggatga
     3781 tttgctgagt gggccgacgg cttgggctag tggtggcagt agtttaattg atcgggataa
     3841 atttttagcc attggtggtt ttgatcaacg ctttaaacca gcttattggg aagatattga
     3901 tttatctttt caagcccgga aaaggggctg gcaagtgctg tttgaagcca aagccgtggt
     3961 ggaacaccac cacgagagca cgcaccaaaa cgtttttggt caagccaaaa ttgagcaaat
     4021 cagtcggcgc aatggcaaaa aatttactaa aaaaaatgcg gatttttggc aaagaatggc
     4081 ttattattta tggcgaccat attggttaat tcaagagtgg cgtcaacccg ccccaaaaca
     4141 acacttaaga cattatttgt ggctggcagc aattttgctg gtggcaatag ggttgcgttt
     4201 ttatcagttg gggagagtgc cagcagggat ggcagttgat gaagtagcga ttggttataa
     4261 cggttttgcg atttggcaaa cccaccgcga tgaatggttg gaattcttac cgatttcttt
     4321 tcgttcttat ggtgattaca aagcgccgtt agcgatctac attaatggtc tgagcactgc
     4381 cctatttggt ttgaatttat gggcggtcag actccccttc gctgtgttta gtatcttggc
     4441 cattgtcggt ttttatctgt tagccaaaga ggtgctggca caaaatcagc tggataaaaa
     4501 tctggctttg tgggcagcgt tatttttaac cttgtctccg tggcacattc actttagtcg
     4561 gttgggtttt gaggcgggga tggctttaag tttgttaatt tgggctgtct acttttggta
     4621 tcgctactgg cgccaacagc gtttgggcca attattactg tcagcgtttt tggcttgttt
     4681 gactttatat acatatcaca gtagcaaagt gactacgcca ttattgtttg tgctaatctt
     4741 tttagcgcag cgccaaagac taacaattaa gcccaaacat ttgctggcgg cgctggcctt
     4801 gactctcttt ttaatcagtc ccttagctta cgacgctatt tatggtgaag gtttaacccg
     4861 cgctggttct agtattatat tgagcgagtt aagtctggct gagaagtgca aagttttatt
     4921 gaccaatgcc ctgtcctatt tttcttggga ttttttagcg agaggtaacg ttaatggtga
     4981 attgcgccat ggtgacgggc agtttggagt cctgagttat ccagccttag ctttgatcat
     5041 tggctatgtc ctcagtttgt tttctaaaaa aattcgcttt aatgaccata gtcgaccaat
     5101 tttagtctta attgggttgt ggttagtggc tggttattta ccagcgatta ttggtgatca
     5161 gccttatcac agcaatcggg ctttgttggc gctgcctgct tggatttttt tggaagtttt
     5221 agcatgtgaa cggctgagcc agttttttca agatcaccgc tgggggttgc actatccgtt
     5281 gcttgttgct tatttattgt ttactttgat ttatcagcgc cattattatc aagactacaa
     5341 tcggctcagt gctgatgctt ttaatgaggg ctacttagaa actgtcgaat atttaaaaca
     5401 attagataaa aatggggtag aaaagatatt atttaccaat gattatcagc acgcttatat
     5461 ttatgtctta tttggcaatc aagtcagccc gattgcctat cacgggggaa ttttagtcaa
     5521 ttacgagttt agtgaagata ttgattttca agatttaaat cgagaaaaga cgattgtcgt
     5581 ggctagtaag gatgatgaga tgatggatta ccagccagat cagattattt atggtagtga
     5641 tggttcacag cgtttccgca tttatctgcc gaaagagtag catcactcta gcgcaagatg
     5701 aacgtttatc tggcaaatta gcatctgtct agcgcaagat gatcattatc acttataata
     5761 aacaaatata tgcagattga agaacaatta agaaccgcga tcagggccag tttacctgag
     5821 aaattagcta agctgattaa tccagaagag attgttttgg aacaacccaa agatagtaat
     5881 tttggtgatt tcagcaccaa tctggccatg cttaaatatg atttagtggc tgatgatttt
     5941 gccagtccgc gggaattggc cgcattgatt gttaagaact ttcagctcca gcagccagcc
     6001 attaccgcca gtgtggccgg ctttggtttt attaatttta aactaagcga cagcattttt
     6061 tggcaaatct ttgctgatct gccacaaagc gaggcggaac taatcccagc aactaagcac
     6121 tcgcaaaaag tgattgtcga atactcgtca cccaacattg ccaagccttt taacattggt
     6181 catttacgct ccacgattat tggtgatgcg gtagctaacc tttatcaagc ggttggttat
     6241 caagtttttc gcgataatca tttgggcgat tggggtacgc aatttggcaa gcagattgtc
     6301 gccattaaaa aatggggaga cgaagaggcg attgccagca gcgccagtcc agtcgctgaa
     6361 ctagtcaaat tgtatgttaa atttcatcaa gaagcggaac aagacgaaag tttgaacgat
     6421 gaggctcgct ggtggtttaa aaaattagag gcaggtgacc aagaggcgcg ccgcttgtgg
     6481 caacgctgta ttgattggtc actgagtgag tttaataaaa tttatcagcg tctcggtgtc
     6541 aaatttacgg aaaatgatgg tcggggttat ggcgaatct
//
LOCUS       DAOSEW010000036         6549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_10277_length_6549_cov_4.360795, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000036 DAOSEW010000000
VERSION     DAOSEW010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 6549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6549
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10277_length_6549_cov_4.360795"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PLJ02_01970"
     CDS             <1..364
                     /locus_tag="PLJ02_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05215.1"
                     /translation="NLKKDQGQGSLFTIYYHGPATILDKNQRHLDETYHQFFLALKNN
                     LDKYQINAKSLTWENDDLLKLDLGKSWLVILDKHSNPETSMRNLSLILLDEAVIAEIE
                     SKGFLDLRFNLPVIREQL"
     gene            420..1742
                     /gene="ftsA"
                     /locus_tag="PLJ02_01975"
     CDS             420..1742
                     /gene="ftsA"
                     /locus_tag="PLJ02_01975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOI05216.1"
                     /translation="MSKNNIISAIDLGTDKCVTIIAKVNEADQLEVLGFSVVPSRGVR
                     RSTIVNLEEALSTVGQSLDAAERMAGLAVKNVVLSVSGVYIRYKNSKGVVPVSSPNQE
                     ISALDVDRVIEAARAISLPSEREIIHVIPKDFKVDSQEGIKDPVGMMGVRLEAETHII
                     TGLSTSLRNFEKCMADLGLNVDAFVFSALAASEIALTETEKELGAVLVDIGAGTTSIC
                     AYVEGVLEFSAVIPVGAKYITQDIASGLRISLEDAEKLKLYLSSDEEVKLIPLPGESK
                     AEFTKRKKKFDLLDPEKVGITNHDPISRKFVTDVVMEPRIKEIFTLVLEELAKAGLLD
                     DAKLPAGIVVTGGGALTYNLIDVARYTTKMSVRLAKPSNILGLTDDIKQANFAVALGL
                     LVYAKRQGNVGAAGGGGDFSLNLKKILPAEFLSGVTEKAKSWFKKIFP"
     gene            1839..3149
                     /gene="ftsZ"
                     /locus_tag="PLJ02_01980"
     CDS             1839..3149
                     /gene="ftsZ"
                     /locus_tag="PLJ02_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011065874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOI05217.1"
                     /translation="MGLVKPSNTTFAKIKVVGVGGGGNNAINSMILSREIKGVEFIAI
                     NTDAQALLTSKAETKLQIGSNFTRGLGAGADPEVGRTAAEESREKIKELLFDTDMVFI
                     TAGMGGGTGTGASPIIAEIAKETGALTVAVVTKPFAFEGVRRMQTAEEGIANLTDIVD
                     TLIVIPNQRLLEVVDKKMTFIDAFRLADNVLGQGVKGISDLITVAGMINVDFADVKTI
                     MTDSGTALMGIGEASGEDRAVMAARMAISSPLLEVSINGAKGILYNITGGPDMTMAEV
                     SDASSIIADSADADADIIFGATIDENMGSKIKISVIATGFSDNYVGSGPRALFGAYGR
                     ESKRKMGHGVVSSVPNPPEEEGFEENKIDRAPEAFTQPQTVADRKPKKEEKIESNNPF
                     VRSALFNLKKGNKQKEADQSDKKEDAEGDDEFSEFEVPAFLRQK"
     gene            3443..6382
                     /gene="ileS"
                     /locus_tag="PLJ02_01985"
     CDS             3443..6382
                     /gene="ileS"
                     /locus_tag="PLJ02_01985"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOI05218.1"
                     /translation="MAKIKITDFKTTPDLAKLEEAILQFWDDEKVFEQSLKQRAGAEN
                     FSFYDGPPFATGLPHYGHLMQSMIKDLVPRYQTMRGKYVRRVWGWDCHGLPIENLIEK
                     ELDLKDKQAIEDYGIAAFNQACQARVLTYAKDWEKTIKRIGRFIDMKNAYKTMDKAYM
                     ESVWWVFSELYQKGLIYQSHKVMYICPRCSTPLSNFEVTQGYKDIKDLAVTAKFKLLD
                     TTDEIYLLAWTTTAWTLPGNMFLAVNPDLDYVLVEEKSTAEKYYLAKSRLEEVFADRD
                     YQIVKTVKGQDLVGLNYQPPFSYFGHLKEDQDLPQAARKLIFTVQAADFVNLEDGTGI
                     VHVAPAFGEDDYQLGESLLNFSNLETAVKNKLFIQHVNFAGRFIEAVTDFAGMEVKPK
                     ADPQATDVAIIRYLAKKQLLFAKQQITHSYPHCWRCDTPLLNYATDSWFVKVSSFKDQ
                     LLKNNQAVNWQPAHIKDGRFGNWLAEARDWAISRNRYWGTPLPLWQSADGDLLCVSSS
                     AELEKLSGQKVANLHKEFIDDIVIKQNGKVYRHLPEVLDCWFESGAMPYAQFHYPFSN
                     QQLFAKNFPANFIAEGQDQTRGWFYTLHVLATALTMSNDQFSASFDKPPSSAFQNVMC
                     TGLIMASDGKKMSKRLKNYPEPEAIIDQYGADSLRLYLLSSAAVKAETLNFEEKEVAD
                     IRRRVFLIWWNILAFYQTFADQQVERLDLQKRQPINHVLDRWLLSRLSSLRQQLVNYL
                     DNYDVMRASRLVAPFINDFSTWYLRLSRERLKAETSQQVSQVFATALAVSAQLFAPIV
                     PFFSELVYQQLQDDDRSIHLSDYPEILADYQDKELETAMNELRQLVELGHAQRKSCQI
                     PLRQPLAAVTFSKPASFKDNAELMAILKQELNVKKVLWGKAQGKDIEVSFDLEITADL
                     AAEGQARQIIRRIQNWRKKAGLKVDQQVPAYLNTWPNQFTKLIEEKTNTQLIRAKIEG
                     LNEQLQD"
BASE COUNT         1963 a         1141 c         1499 g         1946 t
ORIGIN      
        1 caatttaaaa aaagatcagg gtcagggctc actttttacc atttattatc atggaccagc
       61 aacaatttta gacaaaaacc aacgtcattt agatgaaacc tatcatcagt tttttttagc
      121 tttaaaaaat aatttagaca aatatcaaat taatgccaaa agcttgactt gggaaaacga
      181 tgacttatta aaactggatt tgggaaaatc ctggttggtt atcttggata agcacagtaa
      241 tccagaaaca agcatgcgca atctttcatt aattttactt gatgaagcag tgattgctga
      301 aattgaaagc aagggatttt tggatctgcg ctttaattta ccggtcatta gggagcaact
      361 ttgatcgttt ttgttctcga cagtgtcgag caaattctgt atgatgagag ttaactaaaa
      421 tgagtaaaaa taatattatc agcgctattg atttgggtac ggacaagtgt gtgaccataa
      481 ttgctaaggt taatgaagca gatcagttag aggtacttgg tttcagcgta gttccttccc
      541 gtggtgttag acgtagtacc attgttaatt tggaagaagc tttaagtact gttggtcaaa
      601 gtctagatgc tgccgaacgg atggcgggtt tagccgtgaa aaacgtggtg ttatctgtgt
      661 ctggtgttta tattcgttac aaaaattcga agggggtggt gcctgtttct tccccaaatc
      721 aagaaatttc cgctttagat gttgatcggg tcatagaagc agcgcgggcg atttctttac
      781 cgagtgagag ggagattatt catgtgattc ccaaggattt taaagttgat tcgcaggagg
      841 gaattaaaga tccagtcgga atgatgggcg tgcggttaga ggcagaaacc cacattatca
      901 ctggcttatc gaccagtttg cgtaattttg aaaagtgtat ggctgatttg ggtttgaatg
      961 tggatgcttt tgttttttct gctctcgcgg ctagtgaaat agctttaaca gagaccgaaa
     1021 aggagttagg tgctgtctta gttgatattg gtgccgggac tactagcatt tgtgcttatg
     1081 ttgagggggt tttggagttt tcagccgtca ttccggttgg agccaaatac attactcagg
     1141 atattgcttc tggtttgcgt atttcactag aggatgccga aaaactcaaa ctttatttga
     1201 gcagtgatga ggaagtcaag ctaattcctc tcccaggaga atcaaaagct gaatttacta
     1261 aacggaagaa aaaattcgat ttacttgatc cagaaaaagt gggcattact aatcatgatc
     1321 ccatttctcg taaattcgta accgatgtag tgatggagcc gagaattaag gagatcttta
     1381 ctttggtgtt ggaagaatta gccaaagccg gtttactaga tgacgccaaa ttaccagctg
     1441 gtattgtggt gactggtggt ggtgcgctca cttacaattt gatagatgtc gctcgctaca
     1501 cgaccaaaat gtcagtcaga ttggctaaac caagtaatat tttgggatta acagatgata
     1561 ttaagcaggc aaattttgcg gtagccttgg gactattggt ctatgctaaa cgtcaaggca
     1621 atgttggagc agccggtggt ggcggcgatt tttcgctgaa tttaaagaaa attttgcccg
     1681 ctgagttttt gtcaggagtc acggaaaaag ccaaatcttg gtttaagaaa atttttcctt
     1741 aactttgtta aaactgctat ttactagttt acttttatga taaaatcagt taatttgatt
     1801 gtgatagttt gatataaata atagctatat aagaacctat gggactagtt aaaccttcaa
     1861 atacaacctt tgccaaaatt aaagtagtgg gagtgggtgg tggtggtaat aatgctatca
     1921 attcaatgat tctttctcgt gaaattaaag gtgtggaatt tattgccatc aatactgatg
     1981 ctcaagcctt gttaacttcc aaagccgaaa ctaaactgca aattggcagt aatttcactc
     2041 gaggcttagg agctggtgct gatcctgaag tgggtcgcac ggcggcagag gaaagccgtg
     2101 aaaaaattaa ggaattactt tttgatactg acatggtatt tattacggct gggatgggtg
     2161 gtggtaccgg caccggtgct tctcccatta ttgcggaaat tgccaaagaa acgggagcgc
     2221 tgacggtggc agtagtcacc aaaccttttg cttttgaagg cgtacgccgc atgcaaactg
     2281 ccgaagaagg tattgccaat ctcacggaca tagtcgatac gctcatagtt attcccaatc
     2341 aacgcttatt agaagtagtt gataagaaaa tgacttttat tgatgccttc cgcttggctg
     2401 acaatgtact tggccaagga gttaagggga tctctgatct gattacggtg gctggaatga
     2461 ttaatgtgga ttttgctgat gtcaaaacca ttatgactga ttccggaaca gcgctgatgg
     2521 gtattggtga agcttctggt gaggatcgag cggtgatggc agcgagaatg gccatttctt
     2581 ctcccctttt ggaagtatca attaatggtg ccaagggtat tctctacaat attactggtg
     2641 gaccagatat gactatggct gaagtatctg acgcttcctc gatcattgcg gatagcgctg
     2701 atgctgatgc cgatattatt tttggggcga ctattgatga aaacatgggt tcaaaaatta
     2761 agattagcgt aattgccacc ggttttagtg ataattatgt cggcagtgga ccaagagcac
     2821 tatttggagc ttatggtcga gaatcaaaga gaaaaatggg tcacggtgtg gtttcatctg
     2881 tcccaaatcc gcctgaagaa gaggggtttg aagaaaacaa aattgaccgc gccccagaag
     2941 cttttactca accccaaacc gttgctgatc gcaaacctaa aaaagaagaa aaaattgaaa
     3001 gtaacaatcc ctttgtgcgc agtgccttat ttaatctaaa aaaaggcaat aaacaaaaag
     3061 aggctgatca atcagacaaa aaagaagatg ctgagggtga tgatgaattt agcgagtttg
     3121 aagtacccgc ctttttaaga caaaaataat tttgtcgttg ccgaacaagt tgagtgattc
     3181 cgattaaagc aatttagatt aaagtatttt ctgttataat gtcagtataa aagaaaaccg
     3241 tttgagctaa acatcattta gcgaggtgct cgaagaaatt tgacaaacta gttcttgtca
     3301 caaaagtaga acgagtcgca ccgaccgcgt aagagtcaaa atcgagtagc aatagggctg
     3361 gtacctttgc tttaagcaag ccttattaaa gtcgtttaat agcgatcttt attaaggttt
     3421 ttttgttaaa aaaaaaggaa agatggcaaa aataaaaata acggatttca aaactacgcc
     3481 tgatttagcc aagttagagg aagcaatttt acaattttgg gacgacgaaa aagtttttga
     3541 gcaatctctc aaacaacgtg caggggctga aaacttttct ttctatgatg gccctccatt
     3601 cgccactggt ttgccccact atggtcatct gatgcagtca atgatcaagg atctagtccc
     3661 gcgttaccaa acgatgagag gcaagtatgt cagacgggtt tggggttggg attgtcatgg
     3721 tttacctatt gaaaatttaa tcgaaaagga actagatcta aaagataagc aagccattga
     3781 agactatggc attgctgctt ttaaccaagc gtgtcaagca cgagttttaa cttatgctaa
     3841 ggattgggag aaaactatta aacgcattgg ccgttttatt gacatgaaaa acgcctacaa
     3901 aaccatggac aaagcgtaca tggaaagtgt gtggtgggtg tttagtgaac tttatcaaaa
     3961 gggcttaatt tatcaaagcc ataaagtcat gtatatttgt ccccgctgca gcacaccact
     4021 ctctaatttt gaagtaactc agggttacaa ggacattaaa gatttagccg tgacggctaa
     4081 atttaaacta ttggatacca ctgatgaaat ttacttgcta gcttggacta ccactgcttg
     4141 gacactgcct ggaaacatgt ttttagccgt taaccctgat ttagactatg ttttggttga
     4201 ggaaaaaagt accgccgaga aatattattt agccaagtcg cgactagaag aagtatttgc
     4261 tgatcgtgat taccaaatag taaagactgt caaaggtcaa gatttggtcg gcctaaatta
     4321 tcaaccgcct ttttcctatt ttggccattt aaaagaggac caagatttac cgcaggcagc
     4381 gagaaaatta atctttaccg tgcaggctgc tgatttcgtt aatttggaag atggtaccgg
     4441 cattgtccat gtcgctccgg cttttggtga agatgattac caactgggag aaagtttact
     4501 caatttttcc aacctagaaa cggcggtaaa gaataaactt tttattcaac atgtcaattt
     4561 cgctgggcgt tttatcgagg ccgtgaccga ttttgctggc atggaagtca agcccaaagc
     4621 tgatccgcaa gccactgatg tagcaattat tcgctattta gctaaaaaac agctattgtt
     4681 tgctaaacag caaattaccc acagttatcc tcattgctgg cgctgtgata cacccttgct
     4741 taactatgcc actgattctt ggtttgttaa ggttagttcc tttaaagatc aattattaaa
     4801 gaataatcag gcagttaatt ggcaaccagc gcacatcaag gatgggcgct ttggtaattg
     4861 gttggcagaa gctagggatt gggccatttc ccgcaatcgc tattggggaa cgccactgcc
     4921 actctggcaa agtgcagatg gggatctgct ttgtgtttcg agtagtgcgg aactggagaa
     4981 actttctggt caaaaagtcg ctaatttgca caaagaattt attgatgata ttgtgatcaa
     5041 gcaaaatggc aaagtttatc gacatttgcc tgaagtttta gattgttggt ttgagtctgg
     5101 agcgatgccc tatgctcaat tccattatcc atttagcaat caacagttat tcgccaagaa
     5161 ttttcccgct aattttattg ctgagggcca agatcagact cgaggttggt tttatactct
     5221 gcatgttttg gcgacagcgt tgacgatgtc gaacgatcag ttttcggcta gttttgacaa
     5281 accgccaagt agcgcttttc aaaatgtgat gtgtacaggt ttaattatgg ccagtgatgg
     5341 taaaaaaatg agtaagcgct taaaaaatta tcctgaacca gaagcaatta ttgatcaata
     5401 cggggcagat agcttgcgtt tatatttatt gtcttcagcc gcggttaaag ccgagacctt
     5461 aaattttgag gaaaaagaag tagcggatat tcggcgtcga gttttcttaa tttggtggaa
     5521 tattttagct ttttatcaaa cctttgctga tcagcaagtt gagaggttag atttacaaaa
     5581 acgacaacca atcaatcatg tcttagatcg ttggttatta tccagactca gtagtttacg
     5641 ccagcagtta gtcaattatt tagataatta tgatgtgatg cgagccagtc gcttagtggc
     5701 tccttttatt aatgattttt cgacttggta tttgcgatta tctagagaac gacttaaagc
     5761 cgagactagt cagcaagtta gtcaagtctt tgccacagcg ctagctgttt cggctcagtt
     5821 gtttgctcca attgtgcctt tctttagcga gctagtttat cagcaattac aagatgatga
     5881 tcgatccatt cacttaagcg actatccaga gattcttgct gattatcaag acaaagaatt
     5941 agaaacagca atgaacgaat tgaggcaatt ggttgaactg gggcatgctc agcgaaaaag
     6001 ctgccaaatt cctttgcgcc aacccttggc tgcggtgact ttcagcaaac ctgctagttt
     6061 taaagataac gctgaattga tggcaatctt gaaacaagaa ttaaatgtca aaaaggtttt
     6121 atggggcaag gctcagggaa aagatatcga ggttagtttt gatttagaga tcacagctga
     6181 tttggcggca gaaggtcagg ctcgtcagat tattcgtcga attcaaaatt ggcgcaaaaa
     6241 ggcgggttta aaagttgatc aacaagtgcc tgcctacttg aacacttggc cgaatcaatt
     6301 caccaagctg attgaggaaa aaaccaacac gcaattgatt agagcaaaaa ttgaaggatt
     6361 aaacgagcag cttcaagatt aagctagttt caagattaaa gatagctata agaccaagga
     6421 tggttatcaa actaagattg tttcaagatt agtaatagct gattgttgaa aaatagccaa
     6481 ctttgtttta gtctgaaggg gtgcgcaaat attttttacc cagtctgctc ttcatcttca
     6541 tgatcgtgg
//
LOCUS       DAOSEW010000037         6398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_10633_length_6398_cov_4.852751, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000037 DAOSEW010000000
VERSION     DAOSEW010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 6398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6398
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10633_length_6398_cov_4.852751"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1793
                     /locus_tag="PLJ02_01990"
     CDS             <1..1793
                     /locus_tag="PLJ02_01990"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOI05219.1"
                     /translation="LFFLFTFLGLRLIDLQLVRGQELAELAEKNRYFSKSVLTERALI
                     FDRYQQPLVKNHPNYYRLADPQQLYSQQQLIDRDQALFLLATDSAQLNHDFSRVYLYP
                     QPLAHVLGYVTGVTAADLVDNQQLNFFEQFGRMGLESYFDNLLRSSPGTEVYEVDAMG
                     NMQQLVSKTAAIDGESLYTTLDPFLSAVADEALAGKRGAVIIMDAQTGQVLTLLSSPS
                     FDPNLFEAVRIARLRGETDSEAQQTLNSYLTDERQLFFNRAVSGNYPPGSVFKVVTAL
                     AALEEGVVDLSTTVRDEGVLEIGDYQYANWYFTQYGRVEGDINLSRALIRSNDIFFYK
                     TAEWLGPEKLADYARLLGLGQPTGIELGSEASGLVPDPAWKEEQFGERWFLGNTYHFG
                     IGQGDLLVSPLQMARVFQAIFNQGVICRPTLVAGTRECHDLAFNQENLEIINQALLGV
                     TQEGGTAFPLFDYNRSVANVLNQRLGADTVNNMTAREKIKAGMIAGKTGTSEFGTDNE
                     RGYRATHAWFSSVVGLNKQLILDHWQELGMDKASPAQQAWLTALANNQLPQELVITVL
                     VESDGETIYREGSADAAPIVRDILTWMTGF"
     gene            1925..3133
                     /gene="dprA"
                     /locus_tag="PLJ02_01995"
     CDS             1925..3133
                     /gene="dprA"
                     /locus_tag="PLJ02_01995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOI05220.1"
                     /translation="MKQFAKAHLTALWSVPGIGYQSFQRCLLIQKKTRISDEGFWSNQ
                     DQIWQKSMLSKKQAESIIKFKKEHNFTDYYHSLKKKQIRVMVDRERHYPSLLKNIAQP
                     PPVLFYKARAKITADYWQNLFLAVVGTRRVTAYGRFVLKSLVRDVLIASQAKLVSGFM
                     YGVDVEAMKIAQQVTQSIGVLGYGFDYCYPRHQQALMQQMLEEGAIFFSEYPPFIQPS
                     AGSFVQRNRIIAGLARAVLVVEAAKNSGSLITAQFAVDEGRTVLAVPGPITNPYSLAC
                     HQLIREGANLLASVEDFYTALNLSNQEQHISAQEQHLSNQEQPPRTRTDANSQSSVKK
                     SQFLANQALLSDQETTIINILQNQGVALSFDQLSANSQFDHGQLAECLLKLQIKQIIQ
                     QEGQNYVLKT"
     gene            3227..5497
                     /gene="topA"
                     /locus_tag="PLJ02_02000"
     CDS             3227..5497
                     /gene="topA"
                     /locus_tag="PLJ02_02000"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOI05221.1"
                     /translation="MNLVIVESPTKARKLKSYLGGAYTVEASVGHVRDLPKKSLGIDL
                     ENNYQPLYEVSSDKKKVVSKLRKLAREADAIYLAMDPDREGEAIAWHIKYLLEHDRQA
                     KKSETTKRNYLRSTFYEITKPAVLKAIAQPSTINLDLVNAQQARRVLDRLVGYKVSPV
                     LWKKVRRGLSAGRVQSVALRMIVEREREIEAFKSDEYWEFAVALAIPNHFSATELARW
                     QKKEAIFSDNFELELPKEILVLSVEKVDNKKLKIDNEQDAKQVVADLDQAQYQVKDLK
                     QVERRRQSLPPFTTSLLQQSAANRFGFSAKQTMSLAQRLYEEGLITYHRTDAFYLAKS
                     AVESARQYIAREFGAKYVPEKPRYFSQKNKNSQEAHEAIRATDLSITKENLQKVAKLT
                     TNHIKLYDLIWRRFLASQMSDAVYDQTTILVSAKVKDKKKEYELKVVGSVMKFPGWRR
                     LFNQKSDLILPEVKVKQDLIYLAQNPLQKFTQPPARYNDASLIKELEKKGIGRPSTYA
                     SIISVIIQRGYVEREARRFFATPIGVTVSDFLLEHFQVLMDYDFTATIEASLDQIAEG
                     KKAWTKVVDEFYQPLAKRIEAVTKEAERVAVPVEKTGKPCPECAKLVAAGELEQKDAG
                     EVVIRSGRYGKFKSCSRFPDCKYTENLVETLGDQLCPLCQKAPIVIKNTRWGKSFYGC
                     ADYPQCQWASWKKPAPGLKLTQAEWKEIQAKRAAWQAKRAQNKANKAKSKSQSKSKKT
                     SKKSVAKSSKKQKKSS"
     gene            5494..6102
                     /gene="nadD"
                     /locus_tag="PLJ02_02005"
     CDS             5494..6102
                     /gene="nadD"
                     /locus_tag="PLJ02_02005"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOI05222.1"
                     /translation="MRIMLFGGSFNPPHLGHQQMATTVLKVGLADQLWYLPVGKHDFA
                     KDVVSAQQRLAMLELILPQVGEPFFGQMRIEDCEIRYFKTSYTATTLDYLSARYPEHQ
                     FSFLLGSDNLPQFNLWYDQKGRDYHYLLENYPVFVYPRVNYPLEPLDEKMIPLTNLKA
                     VAVSSTQVREALSSGNQQTDLKHLLDERIINYIRKQQLYGTS"
     gene            6089..>6398
                     /locus_tag="PLJ02_02010"
     CDS             6089..>6398
                     /locus_tag="PLJ02_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05223.1"
                     /translation="MAQAKYQIYYQKMIDDNRTLFDRFAKIHQLFEKDQELNAEAFHQ
                     LGKEVLEVIRDYDRRLCAAMGRGVYSVYSDKLSQKFWDLVRADFPKIDLVGVKVKKVT
                     V"
BASE COUNT         1961 a         1112 c         1441 g         1884 t
ORIGIN      
        1 ttttattttt tctttttact tttttgggtt tgcgcttaat tgatttgcaa ttggtgcgtg
       61 gtcaggaatt agctgagttg gctgagaaaa atcggtactt ctctaaaagt gttttgactg
      121 agcgagcttt aatttttgac cgttaccaac agcccctggt taaaaatcat cctaattact
      181 atcgattagc tgatccacaa caactatata gtcaacagca attaattgat cgcgatcaag
      241 ctcttttttt gttagctact gatagtgctc agcttaatca tgatttttcc agagtttatc
      301 tctatcctca gcccttagct cacgttttgg gttatgttac aggagtaacg gcggctgatt
      361 tagtggacaa tcaacaatta aatttttttg aacaattcgg tcggatgggt ctagaaagtt
      421 attttgacaa tttattgcgt tccagtccag gaacagaggt atatgaagtt gacgctatgg
      481 gtaacatgca acaactagtg agtaaaactg cagctatcga tggcgaaagt ttatatacca
      541 cgcttgaccc gtttttatct gcagtagctg atgaggcgtt ggcgggtaaa cgcggagcgg
      601 tgatcattat ggatgctcaa actggacaag ttttgacatt actgagtagt ccgagctttg
      661 atcccaattt gtttgaagca gttagaatag ctcgtctacg aggtgaaact gatagtgaag
      721 cgcaacaaac attaaatagt tatttgactg atgaacgaca gttatttttt aatcgagcgg
      781 tgagcggcaa ttatccccct ggatcagtat ttaaagtggt gacagcctta gctgctttag
      841 aagaaggtgt cgtcgattta tcaacgacgg ttagggatga aggggtcttg gaaattggtg
      901 actatcagta tgctaactgg tatttcactc aatatggtcg ggtagaaggt gatattaatc
      961 tgtcgagggc gcttattcgc agtaatgata ttttctttta taaaaccgct gaatggctgg
     1021 gtcccgaaaa attagccgac tatgctcggt tattgggttt aggtcaacct actgggatcg
     1081 aactggggag tgaagcctct ggcttagtac ctgatccggc ttggaaagaa gagcagtttg
     1141 gtgaaaggtg gtttttgggt aatacttacc atttcgggat tggtcaggga gacttattag
     1201 tcagtccatt acaaatggcc agagtttttc aagccatttt taatcaagga gtaatttgtc
     1261 gcccaacctt agtcgctggt actagagaat gccatgattt ggcttttaat caagaaaatt
     1321 tggagattat taatcaagcc ttgttggggg tcactcaaga gggagggaca gcctttccgt
     1381 tgtttgatta taaccggagt gtggccaatg tcctcaatca gcgtttgggg gccgatactg
     1441 ttaacaatat gaccgctaga gaaaaaatta aagcgggaat gattgctggc aaaactggta
     1501 cttcagaatt tggtacagac aatgaacgag gttatcgagc cacgcatgct tggttttcta
     1561 gtgtagtcgg actcaacaaa caactaattt tggatcattg gcaagaatta ggcatggaca
     1621 aagctagtcc agctcaacaa gcgtggttga ccgctttggc aaacaatcaa ttacctcaag
     1681 aattagtaat taccgtcttg gttgaaagtg atggtgaaac aatttatcgg gagggttcag
     1741 cagacgcagc ccctattgtt agggatattt taacttggat gaccggtttt taattgatga
     1801 caattttttt aatagatgag agtttttttg atagagtagc tagtaatttc gactattgct
     1861 tgtcaaattt tatggatttt tgggatgtca aggttttgtc accaaagtgg ctgataatta
     1921 cttcatgaaa caatttgcta aagctcattt aactgcattg tggtcagtac ctggcatagg
     1981 ctatcagagt tttcaacgtt gcttattgat ccaaaaaaag acccgaatta gcgacgaagg
     2041 gttttggtca aatcaggatc agatttggca aaaatcaatg ctatcaaaaa agcaggcaga
     2101 gtcaataata aaattcaaaa aagaacacaa ttttactgat tattaccata gtttaaaaaa
     2161 gaaacagatt cgggtcatgg tggatagaga aaggcattat ccaagtttac tgaaaaacat
     2221 tgctcagcca ccaccggttt tgttttataa agcgcgggcc aaaatcacgg ctgattattg
     2281 gcagaattta tttttagcgg tagttggtac ccgtcgcgtt actgcttacg gtcgttttgt
     2341 gctgaaaagt ttagtgcgtg atgtattgat cgccagtcag gccaaattag tttctggttt
     2401 tatgtatgga gtggatgttg aagcaatgaa aatagctcag caggttactc aaagcattgg
     2461 ggttttaggc tacggttttg attattgtta tcctagacac caacaagcat tgatgcaaca
     2521 aatgctggaa gaaggagcta ttttttttag tgaatatcca ccttttattc aacctagcgc
     2581 tggcagtttt gtgcaaagaa atcgcattat tgctggttta gccagagcgg ttttggttgt
     2641 tgaagcagcc aaaaacagcg gtagtttgat tacggctcaa tttgccgtcg atgaagggcg
     2701 gacggtgtta gcagtgccag ggcccattac taatccatat tcattggctt gtcaccagtt
     2761 aattagagag ggtgctaatt tattagctag tgtcgaggat ttttataccg ctctgaattt
     2821 atccaatcag gagcaacata tatccgctca ggaacagcat ttatccaatc aagaacagcc
     2881 acctagaaca agaacagacg ccaactctca atcgtcagtg aaaaaaagcc aatttttagc
     2941 taaccaggcg cttttatccg atcaagaaac aacaattata aatatcttgc agaatcaggg
     3001 cgtggcgcta tcctttgatc aattaagtgc taacagtcaa tttgatcatg gccaactggc
     3061 agagtgctta ttaaaattgc aaatcaagca aattattcaa caagaaggac aaaattatgt
     3121 cttaaaaact taagctccat ttgctttttt ttccctaatc cttttaaaat gagggcttag
     3181 tttgcattaa tcactataat acgaagataa taaacgaagg taataaatga atttagttat
     3241 tgttgagtca cccaccaaag ctcgaaagct taaatcatat ttgggtggtg cttacactgt
     3301 agaggcctca gttggacatg tgcgggattt acctaagaaa agtttgggta ttgatttaga
     3361 aaataattat caaccgcttt atgaagtcag cagtgataag aaaaaagtgg tgagtaaatt
     3421 gagaaaatta gccagagaag ctgatgctat ttatctagca atggatccag atcgagaagg
     3481 agaggccatt gcttggcata ttaagtattt gttggagcat gatcgacagg ctaaaaaaag
     3541 cgaaactact aagcgcaatt atttgcgttc aaccttttat gaaattacta aacccgcagt
     3601 attaaaagcg atcgctcagc caagcaccat caatttagat ttggtgaatg cgcaacaagc
     3661 tagaagagtt ttggatcggt tagtgggtta taaagtgtca ccagtactct ggaaaaaagt
     3721 gagacgcggt ttgtcagcgg gaagagtgca atcagtcgct ttgcggatga ttgttgagcg
     3781 cgagagagaa attgaagctt tcaaaagtga tgaatattgg gaatttgccg tggccctagc
     3841 cattcccaat catttttcag caactgaact cgctcggtgg caaaaaaaag aggccatttt
     3901 tagtgacaat ttcgaacttg aattacccaa agaaatttta gttttatcgg tggagaaagt
     3961 tgataataaa aaattaaaaa ttgacaatga acaggatgca aaacaagttg tggctgattt
     4021 agatcaggcg caataccaag ttaaagatct aaagcaggtt gagcgacgtc gccaaagttt
     4081 gccacctttt actacatctt tgttgcaaca gtctgcggcc aatcgttttg gttttagtgc
     4141 caaacaaacc atgagtttgg cgcaacgtct ctacgaagag ggtttaatta cttaccatcg
     4201 taccgatgct ttttatttag ccaagagtgc ggttgagtca gctcgtcaat atattgcgcg
     4261 agagtttggt gccaaatatg tgccagaaaa gccgcgctac tttagtcaaa aaaataaaaa
     4321 ttctcaagag gctcatgagg caattcgggc tacagatctc agtatcacta aagaaaattt
     4381 gcaaaaggtc gccaaactca ccactaatca cattaaactt tatgatttaa tttggcgtcg
     4441 atttttagcc tcacaaatga gtgatgcagt ttatgatcaa acgactattt tggtcagtgc
     4501 caaggttaag gataaaaaga aagaatatga gttaaaagta gttggctcgg tgatgaaatt
     4561 tcctggttgg cgacgcttgt tcaatcaaaa aagtgaccta attttgccag aggtgaaagt
     4621 caagcaagat ttgatttatt tagcgcaaaa tccactgcag aaatttactc aaccaccagc
     4681 tcgctacaac gacgcttcgt tgatcaaaga gttagaaaaa aaaggcattg gacgtccaag
     4741 tacttatgcc tcaattattt ctgtgattat tcagcggggt tacgtggaaa gagaagctcg
     4801 tcgctttttt gccacaccga ttggcgtaac agtgagcgat tttttgttag agcattttca
     4861 agttttaatg gattatgatt ttacggccac tattgaagcc agcctggatc agattgcgga
     4921 gggtaaaaaa gcttggacta aggtggtaga cgaattttac caaccattag ctaaacgcat
     4981 cgaagcagtc actaaagagg ctgaacgagt ggcagtacca gtggaaaaaa ctggcaaacc
     5041 atgtccggaa tgtgccaagc tagtggcagc gggagaatta gagcaaaaag acgctggtga
     5101 agtggtgatc cgctcaggtc gatatggcaa atttaagagc tgtagtcgtt ttcctgattg
     5161 caaatacacc gaaaatttag tggaaacatt gggcgatcaa ctgtgtccac tgtgtcaaaa
     5221 agcaccaatt gttattaaaa acacccgctg gggtaaatct ttttatggtt gcgccgatta
     5281 tcctcaatgc caatgggcat cttggaagaa accagcaccc ggcttaaaat taactcaagc
     5341 cgaatggaaa gaaattcaag ccaagcgggc agcttggcag gctaaacgag ctcaaaataa
     5401 ggctaataaa gctaaaagca aaagtcagag taaaagtaaa aaaactagta aaaagtcagt
     5461 agctaaatct agcaaaaaac agaagaaatc ctcatgagaa tcatgctttt tggtggtagt
     5521 tttaacccac ctcatcttgg tcatcagcaa atggccacaa cagtactcaa agtaggttta
     5581 gctgatcagt tgtggtattt gccagtgggt aagcatgatt ttgccaagga cgtggtatca
     5641 gctcagcaac gtttagccat gttagagtta attttgcccc aagttggtga gccttttttt
     5701 ggtcaaatgc gcattgagga ttgtgaaatc cgttatttca aaactagcta caccgctact
     5761 actctcgatt atttaagtgc ccgttatcca gagcatcaat tttctttttt gttgggtagc
     5821 gacaatctgc cccagtttaa tctgtggtat gaccaaaaag gtcgagatta tcactattta
     5881 ctggaaaatt atcctgtttt tgtttatcct cgagtcaatt atccacttga accacttgat
     5941 gaaaagatga ttcccttgac taatttaaaa gcggtggcag tttcttcaac ccaagtgaga
     6001 gaagcgctga gtagtggcaa tcagcagaca gatttaaaac acttgcttga tgagcgtatt
     6061 atcaattata ttagaaaaca gcagttatat ggcacaagct aaatatcaga tttattatca
     6121 aaaaatgatt gatgataacc gaactttatt tgatcgtttt gctaaaattc atcaattatt
     6181 tgaaaaagat caagagctga atgctgaggc ttttcatcaa ttggggaaag aagttttgga
     6241 agtaatccgt gattacgatc ggcgattatg tgcggcaatg ggtcggggtg tttatagtgt
     6301 ttattcggat aaattgtcac agaaattttg ggatttagtc cgagcagatt ttcccaaaat
     6361 tgatctagtt ggggtgaaag tgaaaaaagt gacagttt
//
LOCUS       DAOSEW010000038         6284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_10926_length_6284_cov_4.994060, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000038 DAOSEW010000000
VERSION     DAOSEW010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 6284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6284
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10926_length_6284_cov_4.994060"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1575)
                     /locus_tag="PLJ02_02015"
     CDS             complement(<1..1575)
                     /locus_tag="PLJ02_02015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOI05224.1"
                     /translation="MRFSMAVKKNYQKVKKKYQPKPKKSKRGRPKLPFYVKWQRFLLD
                     KIKKNKKKIKEKRKKLNKQFFCLATFFGIKKGRGRPKLGPKEKVRRVYQAQKAQFFKF
                     KKAFAKRIHSCRYQFLKLVGLKKVGRPKKRKKLASFPKFLKFSNIKKILFQKKELAYF
                     QIKFKKIKRWRINYSLFGSLVFTIIFLLISYGIYDFVFKDLPSVSDLSTQQQNLTTKI
                     LDRNGQVLYRIYEDENRTLVPLQAVSPDLINATIAIEDKNFYQHFGFSLAGIVRALFA
                     NATSEEIQGGSTITQQLVKSRLLSNERTLQRKLRELLLALVVEGAYEKDQILEMYLNT
                     VPYGGSTYGIEEAAWQYFNKHAKDLNLAESALLAGLPQAPTLYSPFGPNPEVAKARQA
                     DVLRRMVEDGYITQDQANEARVAKLQFREDVVDIKAPHFVMYVKQLLVDLYGEDLVAK
                     GGLEVRTTLDLNLQNEVQQIVSDEVNRLKNLEITNGAALVTNPKTGEILAMVGSADYF
                     DFAHDGQVNVVIRPRQP"
     gene            complement(1646..3823)
                     /locus_tag="PLJ02_02020"
     CDS             complement(1646..3823)
                     /locus_tag="PLJ02_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase"
                     /protein_id="HOI05225.1"
                     /translation="MSAYRQKMKFEDRQLAKGLGEAVAKRTILRTKANGELESWQDVA
                     KRVAEGNSSLLPQGKKWEEHRKKENRLLRKHLSNASLLLSGRHLQHGDASQPERNMEV
                     FTNCSTASSSFLLFYLLLNGSGVGRAYDDALMVVDWDYMPLVRCVLDEHHPDFAWGED
                     ESVKEARHKYRKAHWFEIPDTREGWAQAVEKIEVMTFERKYKDDLLILDFSKVRAKGS
                     PIHGMQGRPASGPRPLIKAIQHVATVKGAGLKPWKQSMYVDHYLSECVLVGGARRAAR
                     IAVKTWTDPDIFEFISIKKGGFLWSANNSVAVDDKFWKQSSKHAQKVLDAVMRSSYFD
                     RTGEPGFVNQHKLVQNDDGFDGYLDGNYASSKKFRPMKRTTKMLAEIAKYASAKYYTQ
                     IPNPCGEITLNMLGGYCVIADVVPYFAPDADSAEEAFRVATRALIRVNTFMDSLYSRE
                     VKRTNRIGVGMTGIHEYAWNAFSYSFHDLIDEKKSLDFWQTIARFKRAVEDEAEKYSK
                     FLGVAVPHTNTTIKPAGTTSKLFALSEGAHLPAMREYIRWVQFRDDDPLVKKYKKLGY
                     PIKELKSYQGTTAVGFPTQPEICRLGMNGKLVTASEATPEEQYQWLMLLEKYWIRGVD
                     ENGKPLPEDKGNQISYTLKYDPEKVSYRKFSSMIRKYQPQIKTCSVMPKIDVTAYEYQ
                     PEEAVTISQFMKVINEISEEDQNTLLEDINMEHLRCESGACPI"
     gene            complement(3834..4283)
                     /gene="nrdR"
                     /locus_tag="PLJ02_02025"
     CDS             complement(3834..4283)
                     /gene="nrdR"
                     /locus_tag="PLJ02_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOI05226.1"
                     /translation="MYCPFCKSKNTAVIDSREAENGEAVRRRRTCEDCEKRFTTYERV
                     EGVDLKVIKKDGSKENFDREKIKRGLLKSTWKRPVTMLQIELLIEEVESKLRQKGVQE
                     VKSWEIGNLVLNRLKKLDPLSYLLFASVYRDFGDLSDFEKEIAKIKE"
     gene            complement(4434..4985)
                     /locus_tag="PLJ02_02030"
     CDS             complement(4434..4985)
                     /locus_tag="PLJ02_02030"
                     /inference="COORDINATES: protein motif:HMM:NF039823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HOI05227.1"
                     /translation="MSKFLKYCLIVLAFLSSIFILLSSFNIASQSQIADAGNFKNRKF
                     YVQKEILPDHSLYPLLMVIDRFRLEMADRERRIYLSVAYAKRRLFYATRLVEKGEMAL
                     ALTTFTKSQKYLNQALLEVQALQTANSYDASAEQLVFFVLEAVDNQQKVIADYLDDFS
                     GAERSVLVNLASETALLRSQLSQ"
     gene            complement(5007..5693)
                     /locus_tag="PLJ02_02035"
     CDS             complement(5007..5693)
                     /locus_tag="PLJ02_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05228.1"
                     /translation="MPSKKRKSKTKKRQSPQQRHQLFWPLLVLIFVFWFLYRILFSFS
                     VFFDELVGKAIFFAFPVLIYLTITGFSATMETLSFAKLKRGLLIGLMLGGVLGFVAVI
                     TKALLNSGHIEPLAFYAAENFWWEMFLAMLTSFFETLFFFSFVMLVIEDRYPKWSLFK
                     KVLVVAFIFLIFHLPNLFLRFSLETIYLHTALLFAFALGQSLLFYAQRNAYTLLMTQA
                     IWGMVLLIHF"
     gene            complement(5668..>6284)
                     /locus_tag="PLJ02_02040"
     CDS             complement(5668..>6284)
                     /locus_tag="PLJ02_02040"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HOI05229.1"
                     /translation="YYKNITISGLPGCGSTTLLSMLREKLKYAGFTGFSGGEFMRAYA
                     AEKGLFNANSKAHHSALVYSDDFDRQVDMGIREKLSNEEGWIIESWLSGFMAQNIPRT
                     LKILMVCSDDAVRIDRIVNRDEISVEEAKNNALTRYQDNLSKWQRMYKKEWDEWLVKT
                     GKVPADAELDFWRPDIYDLVIDTYSHNQQETLKLALDAIKKKKK"
BASE COUNT         1951 a         1350 c         1000 g         1983 t
ORIGIN      
        1 tggctggcgt ggtctaatta ccacattgac ctgaccgtca tgagcgaaat caaaataatc
       61 agcgctaccc accatagcta gaatttctcc ggtcttagga ttggtgacca aagctgctcc
      121 attagtgatt tctaaatttt ttaaccgatt aacttcatca ctgacaattt gttgcacttc
      181 attttgtaaa ttaagatcca aagtagtcct cacttctaaa ccgcctttag ccactaaatc
      241 ctcaccataa agatccacca atagttgttt aacatacatt acgaagtgcg gagccttaat
      301 atcgacaaca tcttctcgaa attgtagttt tgctaccctc gcttcgttgg cttgatcttg
      361 ggtaatatag ccatcctcca ccatccttct tagaacatct gcttgtctag ctttggccac
      421 ttctggattg ggaccaaagg gtgaataaag agtgggagct tgcggtaaac ccgctaacaa
      481 tgcactctca gccaaattga gatccttagc atgcttatta aaatattgcc aagctgcttc
      541 ttcaatccca taagttgaac ctccataggg aacggtgttg agatacattt ctaaaatttg
      601 atctttttcg tatgcccctt ccaccaccaa agccaataat aattcgcgta attttctttg
      661 tagcgtacgc tcattactaa gcaagcgact tttcaccaat tgctgagtga tagtagaacc
      721 accctgaatt tcttcactcg tcgcattagc aaagagggca cgtacaatcc ctgctaaaga
      781 aaagccaaaa tgctggtaaa aatttttatc ttcaatagca atagtagcat tgattaaatc
      841 tggggaaact gcttgtaaag gcaccaaagt ccgattttca tcttcataaa ttcgataaag
      901 cacttgaccg ttgcgatcca aaatctttgt tgttaaattt tgctgttgcg tgcttaaatc
      961 actaactgaa ggcaaatcct taaaaacaaa atcatagata ccataactaa tcaataaaaa
     1021 aattatggtg aaaactagtg aaccaaagag cgaataatta atgcgccaac gtttaatttt
     1081 tttaaacttg atttggaaat aagccagttc ttttttttga aaaagaattt ttttaatatt
     1141 gctgaatttt aaaaattttg gaaaagaggc aagttttttt ctttttttag gacgaccgac
     1201 tttttttaaa cccactagtt ttaaaaattg atatcgacaa gagtgaatac gtttggcaaa
     1261 tgctttttta aacttgaaaa attgtgcttt ttgagcttgg taaacccgtc gaactttttc
     1321 tttgggacct aactttggac gaccgcgacc ctttttgatt ccaaaaaatg ttgctaaaca
     1381 gaaaaattgc ttatttaatt tttttcgctt ttctttgatt ttcttcttat tttttttaat
     1441 tttatccaga agaaatcttt gccatttcac ataaaacggc aacttcggtc gacctcgttt
     1501 tgattttttt ggctttggct gatatttttt tttaactttt tggtaatttt tcttgaccgc
     1561 catgctaaaa cgcattttag cacaccaaga aaaaaaattt gactctaaat taactctaaa
     1621 ttgactaatt agcgttcaat tgattttaaa ttgggcaagc acccgactca catcttaagt
     1681 gctccatatt aatatcttcc aataaggtgt tttgatcttc ttcgctaatt tcgttgatca
     1741 ccttcataaa ttgactaatg gtcactgctt cttctggctg atattcatag gcggttacat
     1801 caattttggg catgacgcta caagttttaa tttgtggttg gtatttacga atcatgctgg
     1861 aaaattttcg atacgacact ttctctggat catatttaag tgtatatgaa atttgattcc
     1921 ctttatcttc tggcagtggt ttgccatttt catcaacacc tcgaatccaa tatttttcca
     1981 acaacattag ccattgatat tgctcctcag gtgtcgcttc actggcggta actaatttgc
     2041 cattcatacc aaggcgacaa atttctggtt gagtggggaa accaacggct gtagttccct
     2101 gataactctt taactcttta attggataac caagtttttt gtattttttc actaacggat
     2161 cgtcatctct aaactgcacc cagcgaatat attcacgcat agctggtaga tgcgctcctt
     2221 cagacagagc aaataatttt gaagtggtac cggctggctt gatagtggtg ttggtatgag
     2281 ggaccgccac tcccaaaaat tttgaatatt tttccgcctc atcttctacc gctcttttga
     2341 aacgagctat agtttgccaa aaatctaagc tctttttttc gtcgattaaa tcgtgaaaag
     2401 agtaactaaa tgcattccaa gcatattcgt gaataccagt catgcccact ccaatccgat
     2461 tagttctttt aacttctctg ctatataaac tatccatgaa agtattaact ctaattaagg
     2521 ctctggtggc tactctaaaa gcctcttcag cactatctgc gtcgggagca aaatatggta
     2581 ccacatctgc aatgacacag taaccaccca acatattaag agtaatttca ccacaagggt
     2641 taggaatttg agtgtaatat ttagcgctag cgtatttggc aatttcggcc aacattttgg
     2701 tggtacgctt catcggccta aactttttgc tactggcgta atttccatct aaatagccat
     2761 caaaaccatc gtcattctgc actaatttgt gttgattaac gaagccaggt tctccagtgc
     2821 gatcaaaata tgaagaacgc atgaccgcat ctaaaacttt ctgagcgtgt ttgctacttt
     2881 gtttccaaaa cttatcatct acagctacac tgttgttagc agaccataaa aacccaccct
     2941 ttttaatact aataaattca aaaatgtctg gatcagtcca agtcttcacg gcaattctag
     3001 ccgctcttct cgccccacca actaaaacac attctgacaa ataatggtca acatacatac
     3061 tttgtttcca aggcttaagt ccagcaccct taactgtggc cacatgctga atcgctttaa
     3121 ttaacggtct aggaccagaa gccggtcgcc cttgcatgcc atgaattggc gaacctttgg
     3181 ccctcacttt agaaaaatct aaaattaata aatcatcttt gtacttacgt tcaaaagtca
     3241 ttacttcaat tttttccaca gcctgagccc aaccttcacg agtatctgga atttcaaacc
     3301 agtgcgcttt gcgatattta tgacgagctt ctttaacact ctcgtcttcc ccccaagcga
     3361 aatcggggtg gtgttcatcc aaaacacaac gaactagtgg catataatcc caatccacca
     3421 ccattaacgc atcgtcatag gcccgaccca caccagaacc attaagcaag agataaaaca
     3481 gcaaaaaact ggaagaggca gtgctacagt tagtaaagac ctccatattt ctttctggct
     3541 ggctagcatc cccatgttgc aaatgccgcc cacttaaaag taaagaagca ttggataagt
     3601 gttttcttag taatcgattt tcttttttac gatgttcttc ccattttttg ccttgaggta
     3661 ataaagaaga gttgccctct gccactcttt tggccacatc ttgccaactt tctaattcac
     3721 cattagcctt tgttcttaaa atggtacgct ttgctaccgc ctcacctaag cctttagcta
     3781 attggcgatc ttcaaatttc attttctggc ggtatgcgct catcttgttc tttctattct
     3841 ttaatcttgg caatttcttt ctcaaagtcg gaaagatcac caaaatctcg ataaacacta
     3901 gcaaataata aataagaaag ggggtctaat ttctttaatc gatttaagac caaattacca
     3961 atttcccaac ttttaacttc ttggacgcct ttttggcgaa gtttggattc aacctcttca
     4021 attaataatt caatttgtaa catggtaact ggtcgcttcc aagtcgattt taacaaacca
     4081 cgttttatct tttcccgatc aaaattttcc tttgaaccat cctttttaat cacctttagg
     4141 tcaacgcctt ccactctttc gtaggtggta aagcgcttct cacaatcttc acaagttctt
     4201 cttctcctga ctgcttcacc gttttccgct tctctagaat cgataaccgc agtattttta
     4261 cttttacaaa atggacaata catttttata tttaagtctg gctatatcgt acttttttaa
     4321 aaaatattct acaatcagtt gtggttgtta gacaaaaaca ccacaaaatg ttgtagattt
     4381 aattagccgg aataaaaata tctcacacaa gctggcgctg tgtcaactag taattactga
     4441 ctcaactgtg acctcaataa agcagtttca ctagctaaat taaccaagac cgatcgttct
     4501 gctccactaa aatcatccag gtaatcagca attacttttt gctgattatc aaccgcctct
     4561 aaaacaaaaa atactaactg ttcggcgctg gcgtcatagc tatttgctgt ttgcaacgct
     4621 tgcacttcga gcaaagcttg attgagatat ttttgcgatt tagtaaaggt agtcagcgcc
     4681 aaagccattt cacccttttc taccaatctg gttgcgtaaa ataagcgtcg tttagcataa
     4741 gctaccgata agtaaatccg tcgttctcga tctgccattt ctaaacgaaa ccgatctatg
     4801 accattaata atggataaag agaatgatcg ggtaatattt ctttttgcac ataaaatttg
     4861 cgattcttga agttacccgc gtctgcaatt tgtgattgag acgcaatatt gaaagaactc
     4921 aacaaaataa aaatcgaact gagaaaggcc aaaacaatta agcaatattt gagaaattta
     4981 gacatatatt tttgcaaaaa taaccattaa aaatgtatta aaagcaccat gccccaaatt
     5041 gcctgcgtca ttaataaagt ataggcgtta cgctgagcat aaaataacaa tgattgacca
     5101 agggcaaacg caaataatag cgcagtatga aggtaaattg tctctaaact aaagcgtaaa
     5161 aataagtttg gcaaatgaaa aattaaaaat atgaaagcaa ctactagtac cttcttaaac
     5221 aaagaccatt tggggtagcg atcttcgatc actaacatga caaaactaaa gaaaaatagc
     5281 gtttcaaaaa aactagtcaa catggctaaa aacatttccc accaaaagtt ttctgccgca
     5341 taaaaagcta atggttcaat gtgacctgaa tttaacaacg ctttagtaat cacagccaca
     5401 aaacccaaga cgccaccaag cattagacca atcaacaaac ccctttttaa tttagcaaat
     5461 gacaaggtct ccatcgttgc cgaaaaacca gtaattgtta gataaattaa caccggaaaa
     5521 gcaaaaaaaa tcgccttacc cactaattca tcaaaaaaaa ctgaaaagga aaataaaatt
     5581 ctgtataaaa accaaaaaac aaaaattaaa actaataacg gccaaaacaa ttgatgtctt
     5641 tgttgcggac tttgacgttt tttagtttta ctttttcttt tttttgatgg catcgagagc
     5701 gagtttcagg gtttcttgtt gattatgaga ataagtatca ataactaaat cgtatatgtc
     5761 tggccgccaa aaatcaagct ctgcgtctgc tggcacttta ccagtcttaa ctaaccactc
     5821 gtcccactct tttttataca tgcgttgcca tttagataaa ttatcttgat aacgagtcaa
     5881 agcattgttc ttggcctctt cgacactaat ttcatctcga ttcacaatac ggtcaatgcg
     5941 gactgcgtca tcactacaaa ccattaaaat ttttaaagtt ctcggaatat tttgagccat
     6001 aaaacctgat aaccaagatt caataatcca accctcctca ttgctgagtt tttcacgaat
     6061 acccatatcg acttgacgat caaaatcatc actataaact aaagcactat ggtgggcctt
     6121 ggagttggca ttaaatagac ctttctctgc cgcataagcc cgcataaact ctccaccaga
     6181 aaatccagta aaaccggcat atttaagctt ttcgcgcaac atacttaata gcgtggttga
     6241 accacagcca ggtaaaccag aaatagtaat atttttgtaa taaa
//
LOCUS       DAOSEW010000039         6233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_11062_length_6233_cov_3.869699, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000039 DAOSEW010000000
VERSION     DAOSEW010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 6233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6233
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11062_length_6233_cov_3.869699"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="PLJ02_02045"
     CDS             <1..518
                     /locus_tag="PLJ02_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05230.1"
                     /translation="EAGKYQAVVFFSKIFFSGQLTCDVKASIPFITSSNCDPNSCNTN
                     ITDPSDNVNYDLCLMQIGNNMPEAFTKCVNCFDSGGIWTAVGCIPQTPEAIVKTLIEI
                     GLIMSGAIVLIMILAGAFMLSTSQGDPKKTQDAKELITSAIIGLLFIIFSITILQFVG
                     VSILRIPGFGE"
     gene            526..1182
                     /locus_tag="PLJ02_02050"
     CDS             526..1182
                     /locus_tag="PLJ02_02050"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HOI05231.1"
                     /translation="MKKRILIAATFILIVLFFTNTSSLKKVHAQNFPTPIVVGCGTIY
                     TDVFNLLGEPVTIYSCTCEEFSKISQNSTSLEYTYCCGWWYPNTCYATEPAGPIATTP
                     SPTAPGSINIPPVDEDLLNTLNPLKNYSTKAEQLSTPGGIISEILAYSFPIAGVILFV
                     MLIMAGFKMLTGASNSSSMEEGKKMISTAVIGFIILFAAYWIAQLIEIIFGIRILGVQ
                     "
     gene            1225..4038
                     /locus_tag="PLJ02_02055"
     CDS             1225..4038
                     /locus_tag="PLJ02_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05232.1"
                     /translation="MLKTVKLVFICLVVFFLSLVTNTELVLAQTATQAATFDLNPEQK
                     DPATYNILPLVLDPNAGNSTYETLLTNMLMDQGYEAHCAQKEWLIDAQAWGDIVDYFA
                     EFPNSSVNLSGTSPQYVNFENARIPLLRGMEASELTDKNDSFEGMFGANLQEVFSALD
                     YENATGVAGRLLSSYWQCFYKKNNLDAIAELCNKFFIDCFLNKEVEIEANPDLNIPAA
                     NLDYLGIKERLDEMHPELASGSKEEKIEKNSAICIDLYPQEDATTDADPTIALNRAAI
                     EKVNFDLDNLYRLAFLVLVPRQSNAGNKLSFLQSDGGAHGKDAPIFIAFKIPEFATNK
                     SRLLDNVDSSELTKIVLQSQAQNTADYENQMIKRNELLEAALEANNLPESNKTIQCAG
                     MPQCQRSRDNVLENALIDIINGAEPVCHTEAISVMELENPSTDLTIDDIVNTNDLNYE
                     RAGDLFTPAVKYFDDTARNPYKNNPLNNELLDRLDSKAETNFSWSVTVNNPQFGLSDG
                     EKIQVKAYLVLPMGETVKDINKAFGIFWNRDAFFELVKTNVLVDMNGKTGAIPKYYTI
                     KNSNASFSDSDGFSWKTNCRLEPDPSGATDLAGNPIYIEVCDEKTFGVTVAGQPEKPL
                     LFPDFGLGWFIKKIQTTIRSTADDAYKYVASCKRVEDLFLGRCSGRLPGELEATCDGT
                     AFAKVEGLPEYAAIPDIAKENFETYVAPKVTQELIDVYGQVEADTGIPCEIIAGVHWM
                     EGGMDPNQSLHDGGPLRGTLLEDATTAMEQLKGKMGMEGVAPDQVQLDYDTLLYGLSS
                     YNGWGNANCASNYLGEERPTRWRLAGYCPASAPGDDHIYPVNWIDDKHQEMDLIFCMD
                     TVEFTCNRASTANDRETIYNRYLEVMGQPPTDEFVENALNLCFMGGGPVCSPELTNNN
                     TNKYPLFQRLGVLTTAIIMNNSH"
     gene            complement(4035..4916)
                     /locus_tag="PLJ02_02060"
     CDS             complement(4035..4916)
                     /locus_tag="PLJ02_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05233.1"
                     /translation="MIDQNQVQRLLEFLSVSKKVGILFANTQQQELLLAILAIYLFLN
                     DLSSLKRTKVNLIKLNLFSPAKIEKSFKSQSNLKKYLKQNQLLAISQQDLGKDNLLIS
                     FPYQPAQVDKVSYHIGENDQRFYLTIKPQAGSAPLNYQEVNFSYTGSSADLLVLIGVN
                     ELEDLQDLYANYRQLYQNTPIITINSFLPDFGTLNLDISGSSGYGEAFFYLIKSLANL
                     LEINLTDLPKIKQIATLLFASIEQKTNQFTSSIMTADSFLAVAELLKLGAERLENTSK
                     AVDNKLPQSKDKKVGPN"
     gene            5013..>6233
                     /locus_tag="PLJ02_02065"
     CDS             5013..>6233
                     /locus_tag="PLJ02_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05234.1"
                     /translation="MRRKFSHFLIFVITFLVSWSLVTKPAQASIWDWIAAQFQSKEAS
                     DNMTTSITQEGVSSEKHVTETSSNLLNVINLHVLGAPTSEDATASSMLRESVGPGLIS
                     VVNSGIVAMYEPPASSRTYMADLLQNAKIIPEAQAQGLGFSSLDPVLETWKSFRNIAY
                     LFFVVLFLVIGFMIMFRAKIGQAAVTVQQAIPSVIVALLAVTFSYAIAGLLIDLMYVI
                     MYMLAALFDGGQELITGNFFTLVGVMFTDFWDNMSTAVEDLMSGLLGQESFLKEALDW
                     LSTLSATVIVGLAILIGTFKIFFELLKSYISIILQIVLAPVILMVGAIPGQNSFMSWI
                     RNLASNLVMWPLVMICLLINRMLTQGTYTSGGFIPPFLIGAGQAETMPILVGIGILLV
                     IPEIMKEAKKKLGVE"
BASE COUNT         1892 a         1151 c         1193 g         1997 t
ORIGIN      
        1 atgaagcggg taaatatcaa gcagtagtgt ttttctcaaa aattttcttt tctggtcaat
       61 taacttgtga tgtcaaggct agtattccgt ttataacttc aagcaattgt gatcccaaca
      121 gttgcaacac caatatcact gacccaagcg ataacgtaaa ctatgatctt tgccttatgc
      181 aaataggcaa caatatgcca gaagccttta ctaaatgtgt taactgtttt gatagtggag
      241 gaatttggac agctgtcggc tgcattcctc aaactccaga agcgattgta aaaactttaa
      301 tcgaaattgg gctcatcatg tcaggagcta ttgttctgat tatgattcta gctggagcat
      361 ttatgctttc aacttctcaa ggtgatccca aaaaaactca agacgctaaa gaattaatta
      421 cttccgccat tatcggtctg ttattcatta ttttttccat aactatttta caatttgttg
      481 gtgtatcaat tctaagaata ccaggatttg gagaataaga aaaaaatgaa aaaaagaata
      541 cttatagcag ccacctttat tttaatagtg ctctttttta ctaacaccag ttcactaaaa
      601 aaggttcatg ctcagaactt tcccacaccg atagtcgttg gttgtggcac tatctacact
      661 gatgtattca atttattagg tgaaccagtg accatttatt cttgtacctg tgaagagttt
      721 agtaaaatta gccaaaactc taccagtctt gaatatactt attgttgtgg atggtggtat
      781 cccaatactt gttacgccac agagccagcc gggccaattg ccacaacccc cagcccaact
      841 gctcctggtt ctattaatat cccacccgtt gatgaagatt tattgaatac tctaaatccg
      901 ctaaaaaatt actccactaa agccgagcaa ttgtcgacac ctggaggcat tatttcggaa
      961 attcttgcct attcatttcc tattgctgga gtcattttat tcgttatgtt aattatggcc
     1021 ggtttcaaaa tgttaactgg agccagtaat tcttcaagca tggaagaagg taaaaaaatg
     1081 attagcaccg ctgttattgg ttttattatt ctttttgctg cctattggat tgctcaatta
     1141 attgaaatta tctttggtat tagaatttta ggtgttcaat aactaaacta gctgctcgtt
     1201 ctttgttctt gtataattgg attcgtgtta aaaacagtta aattggtttt tatttgttta
     1261 gtggtttttt ttctatccct agttactaat actgagcttg ttttggctca aacagccact
     1321 caagcagcca ctttcgattt aaatccagaa caaaaagacc cagctactta caacatctta
     1381 ccgctagtct tagatcctaa tgctggaaat agtacttacg aaactctttt aacaaatatg
     1441 ctgatggatc aaggttatga agcacattgt gcacaaaagg agtggttaat agatgcacaa
     1501 gcttgggggg atatcgttga ttattttgcg gaatttccca acagttcagt caatctttct
     1561 ggcactagcc ctcaatatgt caattttgaa aatgcccgca ttcctttact tcggggtatg
     1621 gaagcaagcg aactgaccga taaaaatgat tcttttgaag ggatgtttgg tgccaaccta
     1681 caagaggtgt tttccgctct tgattacgaa aatgctacag gtgtagctgg tcgcttatta
     1741 agttcttatt ggcaatgttt ttacaaaaaa aataatttag atgcaattgc cgaattatgc
     1801 aacaaatttt ttatcgattg ttttctcaat aaagaagtag aaattgaagc taatcccgat
     1861 ctcaatattc ccgccgctaa tctagattat ttaggtatca aagaacgcct ggacgagatg
     1921 catccggaat tagctagtgg cagtaaagaa gaaaaaattg aaaaaaactc ggcgatttgc
     1981 attgacttgt atccacaaga agatgcaacg acagatgcag atccaaccat tgccctcaat
     2041 cgtgctgcta tagaaaaggt taactttgat ctcgataatt tatatcgctt agcattttta
     2101 gtcttagtgc ctagacaaag caatgcgggt aataaattga gttttttaca aagtgatgga
     2161 ggagctcatg gaaaagatgc cccaattttt attgccttta aaattcctga attcgctact
     2221 aacaaatcaa gattattgga taatgttgat agttcggaac taaccaaaat cgttcttcaa
     2281 tctcaagccc aaaatactgc agattatgaa aatcaaatga ttaaaaggaa tgaattgctt
     2341 gaagccgcct tagaagcgaa taatttacca gaaagcaata aaactattca atgtgcgggt
     2401 atgccgcagt gtcaaagaag tcgagacaac gttttggaaa atgctttaat tgatattatt
     2461 aacggagctg aaccagtatg tcatacagaa gccatcagcg tgatggagct agaaaatcct
     2521 agtactgatt taactataga tgacatcgtc aacaccaacg atcttaatta cgaacgagct
     2581 ggtgacctct ttaccccagc tgttaaatat tttgatgaca ccgctcgcaa cccctataaa
     2641 aacaatccgc tcaataacga attgctggat cgattagata gcaaggcaga aactaatttt
     2701 tcgtggagcg ttaccgtcaa taatcctcaa tttggtctaa gtgatggaga aaaaattcaa
     2761 gtgaaagctt atttggtttt acccatgggt gaaactgtaa aagatattaa taaagcattt
     2821 ggtatctttt ggaatcgaga tgcttttttt gagctagtaa aaactaatgt tctcgtagac
     2881 atgaatggta aaactggagc tattcccaag tactatacta ttaaaaatag taatgcgagt
     2941 ttttctgata gcgatggttt tagttggaaa accaactgtc gcctagaacc tgatcccagt
     3001 ggagcaaccg atttagctgg taatccaatt tatattgaag tttgcgatga aaaaactttt
     3061 ggcgtaactg ttgctggtca gcccgagaag ccactccttt tccccgactt tggcttgggt
     3121 tggtttatta aaaaaattca aaccactatt cgtagtaccg ctgatgacgc ctacaaatat
     3181 gtggcatctt gtaaacgggt cgaggattta tttttgggga gatgtagtgg taggcttccc
     3241 ggtgaacttg aagccacctg tgacggcaca gcctttgcta aagttgaggg tttgccagaa
     3301 tatgctgcga taccagatat tgctaaggaa aactttgaaa catatgtagc tcccaaagtt
     3361 actcaagaat taattgatgt ttatgggcaa gttgaagctg atactggtat tccttgtgaa
     3421 attatagctg gcgttcactg gatggagggg ggaatggatc ccaatcaatc acttcacgac
     3481 ggtggtcccc tacgaggcac cctcttggag gatgctacca ctgccatgga gcaactcaag
     3541 ggcaaaatgg gcatggaggg agtagctcca gatcaggtac agcttgatta tgacactctg
     3601 ctttatggtc tttctagtta caacggctgg ggcaatgcta attgcgcttc taattatctt
     3661 ggtgaagaaa gaccaacccg ttggcggtta gccggctatt gtccagcttc agctccaggt
     3721 gacgatcata tctatccagt caattggatt gatgacaaac atcaagaaat ggatttgatt
     3781 ttttgtatgg acacagtgga gtttacatgt aatcgcgcat ctactgccaa tgaccgtgaa
     3841 actatttaca atcgttatct agaagttatg ggccaaccac cgaccgacga gtttgtcgaa
     3901 aacgctctca atctctgttt tatgggagga ggtccagtct gcagtcctga attgaccaac
     3961 aataacacta ataaatatcc attgtttcag cgccttggag ttttaactac agctattatt
     4021 atgaataata gccattagtt aggacctact tttttatctt ttgattgtgg caatttattg
     4081 tcaacagctt tactggtatt ttccaggcgt tcagctccca atttcaataa ttcagcaaca
     4141 gctaaaaaac tgtccgccgt cattattgaa ctggtaaatt gattggtttt ttgctcaata
     4201 ctagcaaata gtaaagtggc tatttgcttt atttttggta aatcagtcaa attaatttct
     4261 aataaattag ccaaactttt aattagataa aaaaatgctt ccccataacc agaactacca
     4321 ctaatgtcta agtttaaagt gccaaaatct ggcaaaaaac tattaatagt aattattgga
     4381 gtattttgat aaagctgccg ataattggca tacaaatctt gcaaatcttc gagttcattc
     4441 actccaatca gcaccagtaa gtcagcacta gaaccagtat atgaaaaatt tacctcttga
     4501 taattaagtg gcgcactccc cgcttgaggt ttgatagtta aataaaaacg ctgatcattc
     4561 tctccgatgt ggtaagaaac cttatccact tgcgctggtt ggtaaggaaa actaatcagc
     4621 aaattatctt tacccaaatc ctgttggctg attgcaagta attgattttg ttttaaatat
     4681 ttttttaaat tactttgcga tttaaaactt ttctcaattt tcgctggaga aaacagattg
     4741 agttttatta aattaacctt cgttcttttt aaagaagata gatcatttaa aaacaaatag
     4801 attgctaaaa ttgccaacaa caattcttgt tgttgagtat tggcaaataa aataccaact
     4861 tttttactga ctgacagaaa ttctaataat cgctgaactt ggttttgatc gatcactttt
     4921 gatattttta attacttttt gatagcttgt caagcggcaa ataaaaactt cgctttgcac
     4981 aagagacaaa aatcaattaa aatatagctt gtatgagaag aaaattttct cactttttga
     5041 tatttgtcat tactttcctt gtgtcgtggt ctctggtaac caaaccagct caagctagca
     5101 tctgggattg gatagcagcc caatttcaat ctaaagaagc tagtgacaac atgaccacaa
     5161 gcattactca agaaggagtt agtagtgaaa aacacgttac tgaaaccagt tctaatttat
     5221 tgaatgtaat taatttgcat gtgttgggag cgccaaccag tgaagatgcc acagctagta
     5281 gcatgcttcg tgaaagtgtt ggaccaggac tcatcagtgt cgttaattct ggcattgttg
     5341 ctatgtacga accgccagcc agttcaagaa catatatggc tgatttatta caaaacgcta
     5401 agattatccc tgaagcccaa gcccaaggat tgggtttttc ttcactagat ccagtacttg
     5461 aaacttggaa atcatttcgc aatatagctt acctattttt tgttgtactt tttttagtca
     5521 ttggctttat gattatgttt cgcgccaaaa ttggtcaagc tgcggtaaca gtccaacaag
     5581 caatacccag cgttattgtg gcgctactag ccgtaacttt ttcttatgct attgctggac
     5641 tcttaattga tctgatgtac gtaattatgt acatgttggc tgccctattt gatggagggc
     5701 aagaattaat taccggaaat ttctttacgc tagttggggt gatgttcact gatttctggg
     5761 ataatatgtc aacagcagtt gaggatctta tgtctggttt actcggccaa gagtcttttc
     5821 tcaaggaagc attagattgg ttatcgactc ttagtgccac tgtcattgtc ggcttggcaa
     5881 ttctaattgg cacctttaaa attttcttcg aattattaaa gtcttatatt tccattattt
     5941 tacagattgt tttagcacca gtaattttaa tggtcggcgc cattcccgga caaaattctt
     6001 ttatgtcttg gataagaaat ttagcaagca atttggtgat gtggccattg gtcatgattt
     6061 gtttattaat taatcgcatg cttactcaag gaacatatac ttctggtggt ttcatacccc
     6121 catttttaat cggtgctggc caagcagaaa ccatgcccat cttagttggt ataggtatct
     6181 tgctggttat tcccgaaatt atgaaagagg caaagaaaaa gttgggagtt gaa
//
LOCUS       DAOSEW010000040         6223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_11082_length_6223_cov_4.289397, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000040 DAOSEW010000000
VERSION     DAOSEW010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 6223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6223
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11082_length_6223_cov_4.289397"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1718)
                     /locus_tag="PLJ02_02070"
     CDS             complement(<1..1718)
                     /locus_tag="PLJ02_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05235.1"
                     /translation="MPNNFSQLLDLAIKQGYARSSLMTEIKQAIIAKYREQNPKAAIG
                     IAVLIDEESGLIKIFAGNKDLGSEQFSQEASQLAQNILINKIAQGQSGANHQPEASQI
                     DQPVKKNKKTGTGILGKIIFWAYNLYFIFFNLSVAFSLVIGQNFIEVIKSLGWIDVLI
                     FLGLFFLPIVTVIFVIHHGLQKDSTNLTKLLFLFELPLAVLLLLCSSLIGQTTIFTGL
                     SLLALLAIPVVLYVHFAQVKLSALASHIFGFFSQLSTILLGYYALLFAFFLPLILIGL
                     PKEYLSDLFHYGSPYIVNYPALFVKLAFALIIFILIVTITALPYILLRTLWKITEKNR
                     LDLINSLGENRAKKSLAISWVIILILVAVSLFRWPDHPLLAQLSEFDQAADYVTQETA
                     ARPLIEHEEKLQKLVERKVEARSYYLFDKDETFVADAYAQVFGNESFAKVVQTAFTNL
                     AYPLVYWEERADQQALSNNFEYLFGYPYYQNKNELEEVAVRFKEPEEEENKNVLLTYR
                     EITVDSEADGLLARITIEEEYENQTNTEQEIIYEFNLPDEVAFYDLKLGPNLEFSGVI
                     APKGAA"
     assembly_gap    1796..1895
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1987..2601)
                     /locus_tag="PLJ02_02075"
     CDS             complement(1987..2601)
                     /locus_tag="PLJ02_02075"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOI05236.1"
                     /translation="MLGLLFTSPLAFLLILIGLLLSISLHETAHCYVTDKLGDPTPRV
                     MGRLTLDPRAHLDPLGTILIILTGFGWGKASPYDPYNLKSPVRDTAIIAAAGPLTNLL
                     IAILLAIISRLIGGDLIAVFAEFLIYINLSLALFNLLPVFPLDGGKIMRAILPRESSL
                     EYEVTMERYGQFILLFLILPIFNGQSAVTQLLGPIINLIASLLI"
     gene            2696..2926
                     /locus_tag="PLJ02_02080"
     CDS             2696..2926
                     /locus_tag="PLJ02_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011990558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L28 family ribosomal protein"
                     /protein_id="HOI05237.1"
                     /translation="MTKAKISQLIKKLPKVSHTGNNVSHAKNRTKRKFKYNLHAVTVV
                     LNGEKKKIKVPTSQLRQLKRAGLTTHYQKPTE"
     gene            complement(3011..4093)
                     /locus_tag="PLJ02_02085"
     CDS             complement(3011..4093)
                     /locus_tag="PLJ02_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOI05238.1"
                     /translation="MSTNASKQYRLAVAGSTQYSQQIAEQLTKDPAFNLLWVLTPPPK
                     KIGRKQTLTINPLAQWAQERKINTVLVPDNLKNPQLKKQIRSQEVDILLVVDFGYFIP
                     DWLLAFPKITTLNIHPSLLPRWRGSSPGQMALLFQDFQRPWQDNIIGGKKSAVTLMTI
                     SKELDAGDIIVQKKFDIKADWILTDYYQHAFQLMNQSLAKNIKQFIDGELTATAQSQP
                     SPTPFAQSLNKTDAFVAWSQLKKLMTIIPEEKLKTLSDNKSSLSSQKEALLSQLLAGQ
                     GILGNYPLTILEQMQFVNNACRAFQPWPGLWTIVPTHKGEQRLKILSCQIEKLASGQR
                     LVLKKVQLAGKEPCQFKQLKNLIKTD"
     gene            complement(4107..4769)
                     /locus_tag="PLJ02_02090"
     CDS             complement(4107..4769)
                     /locus_tag="PLJ02_02090"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018206 - peptidyl-methionine modification
                     [Evidence IEA];
                     GO_process: GO:0031365 - N-terminal protein amino acid
                     modification [Evidence IEA];
                     GO_process: GO:0043686 - co-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOI05239.1"
                     /translation="MKIISAPHPTLRQVAHPVTKFDKRLAKFVERLKKQLLLEKDPEG
                     VGLAAPQVNKSWQIFVLRHTEHDLTENDQTASNQTEQNQKSQDQTEQDQPQKSKPDDK
                     LDQLTAFINPKIIAHSQKKTLLKETEHFEGCLSVPKIYGPVPRWAWVEVTYQTFSQTG
                     ELETKQEKYQGFLARIIQHEYDHLQGVLFTDHLLENGLPAYVLIKNEWVELEDREILA
                     SF"
     gene            complement(4809..5033)
                     /locus_tag="PLJ02_02095"
     CDS             complement(4809..5033)
                     /locus_tag="PLJ02_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05240.1"
                     /translation="MVEEEMGNQEQAIALLQKTIELKANYWQAYFALGQILDLQGKTA
                     EAANNYRYILENIDPDNDLAQQALAKQNLE"
     gene            complement(5046..>6223)
                     /locus_tag="PLJ02_02100"
     CDS             complement(5046..>6223)
                     /locus_tag="PLJ02_02100"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOI05241.1"
                     /translation="TKSRSGLLALGVALLVQTIAEKFLQNNKKDKKLSLPQLISPLVV
                     VVIVFLLFGRELSLSRPNQSIFSFLTEGLEVNNGNGEEISAPMMTEGGSKSSDIRKVV
                     WQGALKVWQRYPIFGSGVETFAYSYYRDRPVEHNLLSEWDFLYNKAHNELLNFLATTG
                     VVGLATYLALFVALLIYLIKLWRTQQLSSQKIALSVALLAMFISNFFGFSTVTTNLLF
                     SIFAAAIVKETVTWPKITTAKLGWPEYLIAVAILLILTVVNKSIWQIWRADNLFYNAN
                     TYIQDGKWQAGLSLMQEAIEKSPKEAVFYDELATQYSNLAVAFAQEGQATNSAQFAEA
                     AIETSNYVQKLNPVHLNFYKSRARIFIRLAQLNAGFYQAAKETLLKAIELSPTEAKL"
BASE COUNT         1849 a         1161 c         1225 g         1888 t
ORIGIN      
        1 tgcgcagcac ccttgggggc aatcactcca gaaaattcca aattaggtcc aagctttaaa
       61 tcataaaaag ctacttcatc gggcaaatta aactcataaa tgatttcttg ttctgtgttg
      121 gtctgatttt cgtattcttc ctctatagtg atccgagcca ataaaccatc agcttcgcta
      181 tctacagtaa tttcacgata agtgagtaag acatttttat tttcttcttc ctctggttct
      241 ttaaatctaa cagcaacttc ttcaagttcg tttttgtttt gataataagg ataaccaaat
      301 agatattcaa aattgttgct caaggcttgt tgatcggctc gctcctccca ataaacaaga
      361 ggataagcta aattggtaaa ggcggtttga acaactttgg caaaactttc attcccaaaa
      421 acttgggcat aggcatcggc tacaaaagtt tcatctttat caaataaata ataactacga
      481 gcctcgactt tgcgttccac caatttttgc aatttttctt catgttcgat tagtggtcga
      541 gcagctgttt cttgagttac ataatctgct gcttgatcaa attcactaag ttgagctagt
      601 aggggatgat caggccacct aaataaactc acagcaacta aaattaaaat gatcacccag
      661 ctgatagcca gacttttttt tgctctgttt tcacccaaac tgttaatcaa gtctaatcga
      721 tttttttcgg taattttcca gagggtgcgt agcaaaatgt agggtagagc cgtgatggtt
      781 acaatcagaa taaaaataat caaggcaaaa gctagcttca caaacagtgc cggataattg
      841 actatataag gactaccgta gtgaaacaaa tctgataaat attcttttgg caaaccaatc
      901 aaaattagcg gtaaaaagaa agcgaagagc agggcgtaat agccaagcaa gatcgtgctt
      961 aattgactaa aaaaaccgaa aatgtgactg gccagagctg ataatttaac ttgggcaaaa
     1021 tgcacataaa gcaccacagg gatagctaat agtgctaaaa ggcttaatcc cgtaaaaata
     1081 gttgtttgac cgattaaact ggagcaaagt aataataaaa ccgccagagg taattcaaag
     1141 agaaacagca attttgttaa attagtgctg tccttttgca agccatgatg gatgacaaaa
     1201 atgactgtca caattggtaa gaaaaagagg ccaagaaaaa ttaaaacatc aatccagccc
     1261 aaacttttga ttacttctat aaagttttga ccaataacta acgaaaacgc tacagacaaa
     1321 ttaaaaaata taaagtaaag attgtaggcc cagaaaataa ttttacccaa gatcccagta
     1381 ccagtttttt tatttttttt aactggctgg tcaatttggc tggcttctgg ttgatgattt
     1441 gccccgcttt gaccttgagc aattttgttg atgagaatgt tttgtgccaa ttgactagct
     1501 tcttgagaaa attgctccga acctaaatct ttattgccag caaagatttt gattaaacca
     1561 ctttcttcat caatcaaaac agcaatccca atggcagctt ttgggttttg ttcgcgatat
     1621 ttagcaataa tagcttgttt gatctcggtc atcaagctcg accgagcata accttgtttg
     1681 attgctaaat cgagcagttg ggaaaaatta tttggcatat ataaatagtg tagcgatttt
     1741 tgaggctacg cacaagcttt gacgctatac acaagctcta atttcaacac aagctnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntgagg ctacgcacaa gctttgacgc
     1921 tatacacaag ctctaatttc aacacaagct ttaattacaa cacaagctct aattgcagaa
     1981 aatgttttaa attaataaac tggctatgag gttgatgatc ggtcccagca gttgggttac
     2041 ggctgattgg ccattaaaga tgggtaaaat gaggaataac aaaataaatt gaccatagcg
     2101 ctccatagtt acttcgtatt caagactact ttctctgggt aaaatggctc gcataatttt
     2161 gccaccatca agtggaaaaa ccggcagtag gttgaataat gccaaagaca agttgatata
     2221 aattaaaaat tcggcaaaga cggcaattaa atcaccgcca atgaggcgag aaatgatggc
     2281 taacaaaatt gcaatcaaca gattggttag tggaccagcc gcggcaataa tcgccgtatc
     2341 acgtacgggc gacttgaggt tataaggatc gtagggggat gccttacccc agccgaaacc
     2401 agtcaggatg attaaaattg tccccagcgg atccaaatga gcgcgtggat caagtgttag
     2461 gcgtcccatc acccgtgggg ttggatcgcc gagtttgtcc gtcacgtaac agtgagctgt
     2521 ctcatgtaaa ctaatcgaca ataataagcc aattaaaatt aaaagaaagg cgagagggct
     2581 ggtaaataat aatccgagca ttgcttcatt ttactataaa tagcaaaaga tgcatattta
     2641 cttttaatct tgagctaatt gcagaaaaga ttactccatg ttagaattct ctcctatgac
     2701 aaaagctaaa attagccaac taatcaaaaa attgcccaaa gtttcgcata ctggcaataa
     2761 tgtttctcac gccaaaaacc ggactaagcg taaatttaaa tacaatttgc atgcagtgac
     2821 agtggtttta aatggagaaa agaaaaaaat taaagttcct actagtcaat tgcgtcaatt
     2881 aaaaagagct ggtttaacca ctcattatca gaaaccgacg gaataaattt gtcttatatc
     2941 ccttaaatct ttgggaaagt tttgctagcg cccagttgtt ttggtggtta attgtcgtct
     3001 tggcagcgtc ctaatctgtt ttaatcaggt tttttaattg tttaaactga caaggctctt
     3061 tgccagccaa ttgcactttt ttgagaacta aacgttgacc gctagctaat ttttctattt
     3121 ggcaactgag aatttttaat ctttgttcac ctttatgggt gggcacaatc gtccacaagc
     3181 ctggccaagg ttgaaaagct cgacaagcat tgttcacaaa ttgcatttgt tccaagatag
     3241 tgagcgggta attgcctaaa atgccttgac cagccaataa ttgactgagt aatgcttctt
     3301 tttggctact taaggatgat ttattgtcac tgagagtttt taatttttcc tctggaataa
     3361 tcgtcattaa tttttttaat tgggaccaag caacaaaggc atcagttttg tttaagctct
     3421 gcgcgaatgg ggtgggcgat ggttgacttt gagcagtcgc tgttagttct ccatcgatga
     3481 attgcttaat gttttttgcc aaggattgat tcattaattg aaaagcgtgt tggtaataat
     3541 cggttagtat ccaatccgct ttgatgtcaa attttttttg cacaataata tcaccagcgt
     3601 cgagttcttt gctaattgtc atcaaggtca cggcagattt tttaccacca atgatgttgt
     3661 cctgccaggg acgttgaaaa tcttgaaaca acagagccat ttgtcctggt gaactaccgc
     3721 gccagcgggg tagcagtgag ggatgaatgt ttagagtagt aattttgggg aaagctagta
     3781 gccaatcagg gataaagtag ccaaaatcca ccactagtaa aatatcaacc tcttgagagc
     3841 ggatttgttt cttcagttgc ggatttttta aattatctgg aactaatact gtgttaattt
     3901 ttctctcctg ggcccactgg gctaagggat taatggttaa agtttgtttg cgaccgattt
     3961 tttttggcgg gggagtcagt acccagagca aattaaaagc tgggtctttg gtcagctgct
     4021 cggcaatttg ttggctgtac tgagtagagc cagcaacggc caaacgatat tgtttagaag
     4081 cattagttga catgttaatc tttaaattaa aaactggcta aaatttctcg atcttcgagt
     4141 tctacccatt cattttttat caggacataa gccggtagac cattttctag caggtgatcg
     4201 gtaaataaaa ccccttgcag atggtcgtat tcatgctgaa tgattctggc caaaaaacct
     4261 tgatatttct cttgtttagt ttctagttca ccagtttggc taaaagtctg atacgtcact
     4321 tcgacccatg cccagcgagg gactggcccg tagatttttg gtacagacag acagccctca
     4381 aaatgttcag tttctttgag taaagttttt ttctggctat gggcgatgat tttggggtta
     4441 atgaaagcgg ttagttgatc cagtttatca tctggtttgc ttttttgggg ttgatcttgc
     4501 tctgtttgat cttggctttt ttgattttgc tctgtttgat ttgacgcagt ctgatcattc
     4561 tcggtaagat cgtgctcggt gtggcgtaaa acaaaaattt gccaggattt attgacttga
     4621 ggtgcagcta aacccacgcc ttcaggatct ttttccagta acagttgttt tttgagacgc
     4681 tcaacaaact tggccaatct tttatcaaat ttagtgacgg gatgagccac ttggcgcagg
     4741 gtaggatgag gagcgctaat aattttcatc tgggctatta tacctcaaaa agcttgcttt
     4801 attaatcttt attccaaatt ttgcttagct aaagcttgtt gagctaaatc attatctgga
     4861 tcaatatttt ccaatatata tcgataatta ttggctgctt cggcggtttt gccctgtaaa
     4921 tctaagattt gaccaagagc aaagtaagct tgccaataat tagctttcag ttcgattgtt
     4981 ttttgaagca aggcaatggc ttgttcttgg ttgcccattt cttcttccac caaagctaag
     5041 ttgtattaaa gtttggcttc agtgggtgac agctcgatgg cctttaataa ggtttctttg
     5101 gcagcctgat aaaacccggc attgagttgg gctaatctga taaaaattct tgctcgactc
     5161 ttataaaaat taagatgcac cggattaagt ttttgtacat aattactggt ttcaatggcc
     5221 gcttcagcga attgggcact attggtggct tgaccctctt gggcaaaagc aacggctaaa
     5281 ttgctatatt gagtagctaa ttcatcataa aagactgctt ccttgggaga tttttcaata
     5341 gcttcttgca tcaggctgag gcctgcttgc catttgccat cttgaatata agtattggca
     5401 ttataaaaga gattatccgc tcgccaaatt tgccaaatac ttttgttgac caccgttaaa
     5461 attagtaaaa tcgcgactgc tatcagatat tctggccagc ccaacttggc tgtggtaatt
     5521 tttggccaag tcactgtttc tttaactatg gcagcagcaa aaatactaaa taataaattg
     5581 gtcgtcaccg tagaaaaacc aaagaaattg ctgataaaca tcgccaacaa ggccacactt
     5641 aaggcaattt tttggcttga tagttgttga gttcgccaaa gtttaattaa ataaatcaat
     5701 aacgctacaa acaaagccaa ataggtagct aagcccacca ccccggtggt ggccaagaaa
     5761 ttaagtagtt cattatgagc tttgttatat aaaaaatccc attctgacag cagattatgt
     5821 tcaactggtc gatctcgata ataactataa gcaaaggttt ctacacctga accaaagatg
     5881 gggtagcgtt gccaaacttt taaggctcct tgccaaacca ctttacgaat atcgctcgat
     5941 ttagagcctc cttctgtcat catcggtgca ctgatttcct caccattgcc attgtttact
     6001 tctaaacctt cggtcaaaaa gctgaaaatt gattgattgg gtcggcttaa actcaattct
     6061 cggccaaaga ggagaaaaac tataaccact acaactagcg gtgagatcaa ttgggggagg
     6121 ctgagttttt tatccttttt gttgttttgc aaaaatttct cagcgatggt ttgcaccaat
     6181 aaagcaacgc ccagagccaa aagaccagat ctggatttgg tga
//
LOCUS       DAOSEW010000041         6199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_11131_length_6199_cov_3.870768, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000041 DAOSEW010000000
VERSION     DAOSEW010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 6199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6199
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11131_length_6199_cov_3.870768"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..1075
                     /locus_tag="PLJ02_02105"
     CDS             227..1075
                     /locus_tag="PLJ02_02105"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOI05242.1"
                     /translation="MTITYFIFYSLFVLLFFFFGAALASFLNVVIYRTAANCGLLEKS
                     KGQVLKLPPFWQGRSLTDCCQQPIKAVHNIPVISFVLLRGKCPYCQAKIAKKHLLTEL
                     VAGLYTLVFVCLTYLQINNGQAVDPLMVAAQYLFFLLLIFVVIADFQYLIIPDFFIIL
                     LSIFALFLAGVNWPNGFIAILASSLFFTLLYLGASFIFKKEALGLGDIKLMLPLSFVL
                     GWPKVLLAIFLSFIVGGFFATLLLLLKQKKIGQTLPFGPFLVVGFVLSYFWAETIWAW
                     YMALII"
     gene            1072..2097
                     /locus_tag="PLJ02_02110"
     CDS             1072..2097
                     /locus_tag="PLJ02_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05243.1"
                     /translation="MRIIRFLSSSLIFIIFFLLIGIFFGREILLIMATSDLKRAANAL
                     LSASHLVNCYDGFSRSPSAWGQLRFVDDSNYVLETVCSDFPKRPILIESKKLPIFVKK
                     VNGASGFIFNSQKPIASQITIRSLGKDQLIYKEINDLESGQPPAVLDYVAGPASTCQA
                     FNFQCCQEDIELGVGVSQTLATDCPKDCYQSCQPRPLVVSFNAMPASLENRRIIGVRS
                     GQTVTFAYVVSEGNQELFSGQVMVGDTKDSSFLQWKSRWQTLVQLINNLEDNPVKQVQ
                     LPITSTIFFGDGQSQTSQSLQGVIDHVYTCSEAVCYFNAWIEAQDARLTKSADNELTR
                     LQIIVSN"
     gene            2105..2539
                     /locus_tag="PLJ02_02115"
     CDS             2105..2539
                     /locus_tag="PLJ02_02115"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOI05244.1"
                     /translation="MKHNKLKAFTLLELLVVIAIIGILISLGVASFGNAQRKSRDSRR
                     REDMKAVQNGLEQYYADHGGVYPTGSTMAAVINAAGTSYFPAGAPTDPKITQSYGIRS
                     SANGFCACAFLETPGAGNATAMPTSISCTFGTGNYFCVSNLQ"
     gene            2560..2970
                     /locus_tag="PLJ02_02120"
     CDS             2560..2970
                     /locus_tag="PLJ02_02120"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOI05245.1"
                     /translation="MQKNKIKNRAFTLIEVLVVATIIGVLVTVTAVSFANSQKRSRDA
                     RRKADLETVRQALVLYRQDHGSYGNVSGGFATVVSNLYTGGYLTANTIADPKTGNPAY
                     SLSCVNGAATNCNKVQLNASLENEVPAAYAVLTP"
     gene            2933..3451
                     /locus_tag="PLJ02_02125"
     CDS             2933..3451
                     /locus_tag="PLJ02_02125"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOI05246.1"
                     /translation="MKYLPLMPFSRHKSSAFSLLELLVSVTIMMLMVGGALAGYTSYT
                     EKQRLVAAAEKLESGLREAQNMARTGYLGTCDELGYVDFSMYIDATDGLIYRIRLHCA
                     EDATDTYRSANTKYETVGQDFMLSEDLLSLKFYPYGNLSNDVSRTLSSTRTNYQAVFE
                     IDQGGGIKVTYN"
     gene            3463..3963
                     /locus_tag="PLJ02_02130"
     CDS             3463..3963
                     /locus_tag="PLJ02_02130"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOI05247.1"
                     /translation="MLKKRTNQHQKQRAFTFVEVLVATMLVALVMTALGSMMALSMRV
                     AEANEMEQLAQLKAQQTMEFFRRERLIRGWSDFYNSLQDNATYCLNTLPANITTISPG
                     SCFTYKSDFNFFYYSVEANIDRTNLPGRQSVIVEVNIYRYDMSGNLIGDPGVAFFTVN
                     QTFKQY"
     gene            complement(3960..4484)
                     /locus_tag="PLJ02_02135"
     CDS             complement(3960..4484)
                     /locus_tag="PLJ02_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05248.1"
                     /translation="MLKKTAQHQPLAAFTLVEILVTVTLTSVIMLGITSLFISFMSSA
                     GKSRISQSVRENGTMAMQRMIEELRNAKAINLSAAQCNGSTAYSSLSFVNADDITSTL
                     AASSNRIKLNTGGSDYYLTSDSNQLRNLGFTCYNTEAAKYIEISFTLASSTAIANSPT
                     HSQLDFKSGVALRN"
     gene            complement(4503..>6199)
                     /locus_tag="PLJ02_02140"
     CDS             complement(4503..>6199)
                     /locus_tag="PLJ02_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05249.1"
                     /translation="TWGICYEAQDENGVHIPCDSNEVCRTYNDGAYPTDICVIDTSLI
                     CKIQENPVIKGCCYDSGEPLPTLIPTFTPVPTSTPVPTGYISPTPTPPPPSPTLNPSI
                     PPPYAVTINLHFIQSSSASLSGGVCTGPTTPGYTDTTNINWGGYTQQNVGDSIFSFGL
                     TGVYEDSVFTIQAWPASANFGCSCSNSPLAGEENRCLYADVGVPPRSNPNLDLYFYFD
                     QANYPESWFQTFGASAFARNSITSIVPFATCAAETGKCQPAIFAPRPTLTSGLTSGFA
                     LLGNTNHLQLLSDENTGLSTRFQNLHWAGQRSPNQDAFGLGLNADSYSLSYNYFYTLA
                     DNASTIRGPLTQAQTNLATWRANGWINGGQVTNYFLVNESLTLDENDNFFVNNGESVV
                     VFVNGNLTINNASGNDDTKITSVARKTATNGGGFLAFIVSGNIIINSNVGKTINPNQT
                     SFIQPVTYANSQLEGVYVANGAITVNSQGNDYLNGGYPDRKLIAAGTFVGLTGINLNR
                     RADNPADPNTIYYKIQNSYQALENFIYRADLLVNFPKELKSSVINWREVAPRSFNE"
BASE COUNT         1740 a         1138 c         1375 g         1946 t
ORIGIN      
        1 tatgattcta cctatgtctg ttttgcacct cagtccaatg cttttcaaca ggaagcagct
       61 gaacgcgtga ctgcaggttt accaactgat tatccagttg ctgatgcagt tggcaacaca
      121 actgactgtg ggacacaggg taattgtatc tgtcttccct aatcggttta gttaaaaaag
      181 taaaacccga gccccaaggc tcgggttttt ttgttattat tgagtggtga caataactta
      241 ttttatcttt tattcgctct ttgttttact atttttcttt tttggagcgg ctctagcgag
      301 ttttttgaat gtagtgatct atcgtacggc ggctaattgc ggtttgcttg aaaaaagcaa
      361 agggcaagtg ctcaaattac cccctttttg gcaaggtaga tcgctaaccg attgttgtca
      421 gcagcccatc aaagcggtgc ataatattcc ggtaatttct tttgttctac tgaggggaaa
      481 atgcccttat tgtcaagcta aaattgctaa aaaacatctg ctaacggaat tggtggctgg
      541 actttacaca ttagtttttg tttgtttaac ctacctgcaa atcaacaatg gacaggcggt
      601 ggatcctctc atggttgcgg ctcaatattt attctttttg ctgttaattt ttgtggtgat
      661 tgccgatttt cagtacctca ttattcctga tttttttatc atcttattga gtattttcgc
      721 gctcttttta gctggggtca actggccaaa cggtttcatt gccattttag cctctagtct
      781 tttctttact ctactctatt tgggggcgtc atttattttc aaaaaagaag ctttaggttt
      841 gggtgatatt aaattgatgt tgccactgag ttttgtgcta gggtggccga aggtattact
      901 agcaattttt cttagcttta ttgttggcgg attttttgct acacttttat tgctacttaa
      961 acagaaaaag attggtcaaa cgctgccttt tggacctttc ttggtggtgg gctttgtact
     1021 aagttatttt tgggcagaaa caatttgggc ttggtacatg gctctaatta tatgagaatc
     1081 atacgttttc tctcctcatc tttgatattt atcatttttt ttctgctcat cggcattttt
     1141 tttggtcgag aaattttact gattatggcc actagtgatt tgaaaagagc agctaatgct
     1201 ttgttgtcgg ctagtcattt ggtgaattgt tatgacggtt tttcccgttc tccatctgct
     1261 tgggggcagc ttcgttttgt ggatgacagc aattacgtgt tggaaactgt ctgcagtgat
     1321 tttcctaaac gaccaatttt gatcgaaagt aaaaaactgc ccattttcgt taaaaaggtt
     1381 aatggtgcct ctggttttat ttttaattct caaaaaccta ttgctagtca gattaccatt
     1441 cgtagcttgg ggaaagatca gttaatctat aaggaaatta atgatttaga aagcggacaa
     1501 ccacctgccg ttttggatta tgtggcgggt ccagccagta cctgtcaggc ttttaatttt
     1561 caatgttgtc aagaagatat agaactggga gtgggcgtta gtcaaacatt ggctactgat
     1621 tgtcctaagg attgttatca aagttgtcag ccacgaccgc tagtggtctc ttttaatgcc
     1681 atgccagcta gtttagaaaa ccgcagaatc attggtgtca gatctggaca aactgtgact
     1741 tttgcttatg tggttagtga aggcaaccag gagctattta gtggtcaagt gatggttggt
     1801 gataccaagg atagctcgtt tttgcaatgg aaaagtcgtt ggcaaacctt ggtgcaatta
     1861 attaacaatc ttgaagataa ccccgttaaa caagtgcaat tacctatcac cagcactatt
     1921 ttctttggtg atggccaaag tcaaacttcg caaagtttgc aaggagtgat tgatcatgtg
     1981 tatacttgta gtgaagcagt ttgctatttt aacgcttgga tagaagccca ggatgcgcgc
     2041 ttgaccaagt cggcggataa tgaattaact agattgcaaa tcatagtaag taattaggaa
     2101 atttatgaaa cacaataaac taaaagcgtt tactcttctt gaattgttag tggtgattgc
     2161 catcattggt attttaattt cactgggtgt ggctagtttt ggtaatgctc agcgcaaatc
     2221 tcgagatagt cgtcggcgtg aagatatgaa agcagtacaa aatggtttag aacagtatta
     2281 cgcagatcat ggtggggtgt atccaactgg ttcaactatg gcagctgtaa ttaatgctgc
     2341 tggtacatcc tattttcccg ctggagcgcc tactgatcca aaaattactc agagttatgg
     2401 aatcagatcc agtgcaaacg gtttttgtgc ctgtgctttt ttagaaactc ctggcgccgg
     2461 gaatgctaca gctatgccaa ctagcatcag ttgtactttt ggtactggca attatttttg
     2521 tgtcagcaac ttacaataac aatagcaaaa aagtacatta tgcaaaaaaa taagataaaa
     2581 aatcgggcat tcaccctgat tgaagtacta gtggtagcaa caattattgg ggttttagtg
     2641 actgtgacag cagttagttt tgccaattcg caaaaaagat ctagagatgc tagacgcaag
     2701 gcagatctgg aaacagtgcg ccaagctttg gttctatata ggcaagatca cggcagttat
     2761 ggtaatgttt ctgggggatt tgccacggtt gttagtaatt tatatacagg tggatatttg
     2821 acagcaaaca ctattgctga ccccaaaact ggcaatccag catatagttt gagttgtgtc
     2881 aacggtgctg ctaccaattg taataaagta cagttaaatg ctagtttaga aaatgaagta
     2941 cctgccgctt atgccgttct cacgccataa atcctctgct ttttctttgt tagagctact
     3001 agtgtcagtg acgataatga tgttgatggt gggcggagct ttggctggat acaccagtta
     3061 tacagaaaaa cagcgtttgg ttgcagcagc tgaaaaactt gagtctggtc tgagagaagc
     3121 ccaaaatatg gcaagaactg gctatttggg gacttgtgac gaattaggat atgtcgattt
     3181 ttccatgtat attgatgcca cagatggttt aatttatcgg atccgcttgc actgcgccga
     3241 ggatgcgacc gatacttatc gatcggcaaa tactaaatat gaaactgttg gtcaagattt
     3301 tatgctcagc gaggatttgt tgagtctaaa attttatcct tatgggaacc taagtaatga
     3361 tgtttctcga acgctcagct ctactcggac taattatcaa gctgttttcg aaattgatca
     3421 aggaggcggg atcaaagtga cttacaacta aaatttatct ctatgttaaa gaaaagaact
     3481 aatcagcacc agaaacaaag agcttttact tttgtagagg tgctagtagc taccatgtta
     3541 gtggctctgg taatgaccgc tttgggctcg atgatggcgc tctcaatgag agtggcagaa
     3601 gccaacgaaa tggagcaatt agcccaactc aaagcccaac aaacaatgga attttttcgt
     3661 cgagaacgac tgattcgagg ttggagtgat ttctataaca gtttgcaaga caatgccaca
     3721 tattgtttga acactttacc tgccaatatc actactattt ctcctggtag ctgtttcaca
     3781 tataaaagtg atttcaactt cttttactat agtgtggaag ctaatattga tagaactaat
     3841 ttacctggtc ggcaaagtgt cattgtagaa gtcaatatct atcgctacga catgtctggg
     3901 aatctaattg gcgatccagg tgtagctttt tttaccgtca atcaaacttt taaacaatat
     3961 taattgcgca aagcaactcc agatttaaag tctagttgac tatgagtggg gctattggca
     4021 atagcggtgc tactagctag cgtaaaacta atttcaatat atttggcggc ttccgtgttg
     4081 tagcaggtga agcccaaatt tctaagttga ttgctatcgc tagtcaggta gtaatcggaa
     4141 ccaccggtgt ttaatttaat gcgattactt gaagcagcta aggtgctagt tatatcatca
     4201 gcattaacaa aagataaact agaatatgcg gtgctaccat tgcactgggc tgccgataaa
     4261 ttgatggctt tggcattgcg taattcttcg atcattctct gcatggccat cgtcccattt
     4321 tctctaacac tttgactgat acgacttttt ccagcagatg acataaaact aataaataag
     4381 ctagtaatgc ctagcataat gaccgaagtc agtgtgacag tgactaagat ttcaaccaaa
     4441 gtaaaagcag ctaaaggttg atgctgggct gtttttttta acattacaat caatttgctt
     4501 agttattcat taaaacttct tggcgcgact tctcgccaat taataacact agatttcaat
     4561 tctttgggga aattaactaa taaatcagct cgatagataa agttttctaa agcctgataa
     4621 ctattttgaa ttttgtaata gattgtattt gggtctgccg ggttatcggc tcgacgattt
     4681 aaattgatgc ctgttaatcc aacgaaggtg ccggcagcta ttaattttcg atcaggataa
     4741 cccccgttta ggtaatcatt accttggcta ttaactgtga ttgcgccatt agccacatac
     4801 actccttcca gctgagaatt ggcataagtt actggttgaa taaagctggt ttgattagga
     4861 ttaatagttt taccaacatt agagttgata ataatattgc cactgacaat gaaagctaaa
     4921 aagccaccac cattggtggc agtttttcgg gcgacactag taattttggt gtcatcattt
     4981 ccactagcgt tgttaatggt cagattgcca ttaacaaaaa ccaccacact ttcaccatta
     5041 ttgacaaaga aattatcatt ttcatctaaa gtaagacttt cattaaccaa gaagtaattg
     5101 gtaacctgac caccattaat ccaaccatta gctcgccaag tggccaaatt agtttgagcc
     5161 tgagtcaatg gtcccctgat cgtgcttgca ttatcggcca gtgtgtaaaa ataattgtag
     5221 cttaggctat aactgtcggc attaagaccc aaaccaaaag catcttgatt gggtgatcgt
     5281 tggcctgccc agtgtaagtt ttgaaaacga gtgctcagac cggtgttttc atcgcttaag
     5341 agctggaggt ggttggtatt gcccaagaga gcaaaaccac tagttagacc actggtcaga
     5401 gtcggtctgg gggcaaaaat ggcgggttgg cacttgccgg tttcagctgc acaggtggca
     5461 aatggcacga tagaggtaat gctgtttcta gcgaaagcac tggctccaaa agtttggaac
     5521 caagactcag gataattagc ttgatcaaaa tagaaatata ggtctaggtt aggatttgag
     5581 cgaggaggta cccctacatc tgcataaaga caacggtttt cttcacccgc aaggggacta
     5641 ttggaacaac tacagccaaa attggcactc gctggccagg cttgaatggt aaaaacacta
     5701 tcctcgtaga cgccggttaa tccaaaggaa aatattgaat cgcccacatt ttgttgagtg
     5761 tagccacccc aattgatatt ggttgtatca gtatatcctg gagtagttgg cccggtgcag
     5821 acacctccag aaagcgaggc gctgctagat tggataaaat gtaaattgat agtgactgcg
     5881 tagggagggg ggatactcgg attgagggtt ggcgaaggtg gtggtggagt tggagttggc
     5941 gaaatatagc cagtaggcac tggtgtggaa gtaggcactg gtgtgaaagt aggtattaat
     6001 gttgggaggg gttcaccact gtcataacag caacctttga tcacaggatt ttcttggatt
     6061 ttacaaataa gtgaggtgtc aattacacaa atatcagtgg gataagcacc atcattatag
     6121 gttcgacaga cttcattgga atcgcagggt atatgtaccc cattttcatc ttgagcctca
     6181 taacaaatac cccaagtat
//
LOCUS       DAOSEW010000042         6167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_11202_length_6167_cov_3.754254, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000042 DAOSEW010000000
VERSION     DAOSEW010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 6167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6167
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11202_length_6167_cov_3.754254"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..381
                     /locus_tag="PLJ02_02145"
     CDS             193..381
                     /locus_tag="PLJ02_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05250.1"
                     /translation="MINQKNFNRLVFFLLIMLALAAGYFQSALETQERRYQALDEHCQ
                     KLELMLTASNSAQLELEF"
     gene            396..1004
                     /locus_tag="PLJ02_02150"
     CDS             396..1004
                     /locus_tag="PLJ02_02150"
                     /inference="COORDINATES: protein motif:HMM:NF013103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HOI05251.1"
                     /translation="MQEIIYYQPQEDLALAENIYRTAIDGLYYVRYQKFIDERGLFAQ
                     VLEPARITQQINPHFTIKQVNLSVSKTKVVRGIHAENWNKLITVLSGRAQLVIADIRP
                     HSITYKETIYFDFNADNNQQWGEALYISAQLGNSICALQGPVYYLYGVDQLYQDRNQA
                     DDQAISLFDPDLAIKWPFPKEEMIISKRDLASITLKELEKKD"
     gene            1006..2028
                     /gene="rfbB"
                     /locus_tag="PLJ02_02155"
     CDS             1006..2028
                     /gene="rfbB"
                     /locus_tag="PLJ02_02155"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015229365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOI05252.1"
                     /translation="MANTILVTGGAGFIGSNFIHYWLKHHPEDHLLNLDALTYAGNLA
                     NLQTVANHSNYHFVQGNICDRPLVNQLMKDVDIVVHFAAESHVDRSILDPALFLETNV
                     IGTQVLLEAALKNKIKRFHHISTDEVFGALPLTSQEKFNKHTAYDPHSPYSASKAAAD
                     HLVRAYGDTYGLPVTISNCSNNYGEYQFPEKIIPLAITNLLDGQKVPIYGDGLYVRDW
                     LYVQDHCRGIDAVLNRGKVGETYLFGGLYKDISNLELIKMIIKLMNQDESAIAMVKDR
                     PGHDRRYSVDFSETEKALDWRPSVDLETGLRRTIEWYQTNEAWWRPLKEKNQAYFQQQ
                     YGKTND"
     gene            2021..2902
                     /locus_tag="PLJ02_02160"
     CDS             2021..2902
                     /locus_tag="PLJ02_02160"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HOI05253.1"
                     /translation="MTKQHILGTGLNGLVGSKFVSDFSDRYLFDNLDLRDKQRPVDIT
                     QQQQVESVIAQSAAKFLIHFAAFTNVTAAFAQSGDKNGLCYQVNVVGTKNIAQACQKF
                     NKHLIHISTAYVFNGEKEGLYTEQDPLSPIEWYGQTKAWAEEVVQSTPGLTWTILRID
                     QPFRSDPFAKLDVAWKLIEGMRAGKIYPQFTDHYFGPTYIDDFAKVLDAVIRLNLTGL
                     YHATSGEQWTDFDFARSLAQQLHIDYQVEAGSLAKYLETSQRPYQKNTALSVDKLKSV
                     LDFSLTPITTAMSHILA"
     gene            complement(2894..3547)
                     /gene="trmD"
                     /locus_tag="PLJ02_02165"
     CDS             complement(2894..3547)
                     /gene="trmD"
                     /locus_tag="PLJ02_02165"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOI05254.1"
                     /translation="MQINFLTIFPEYFSNILDSSILKRAQQKQLVKYQVINLRDFAQD
                     KHHTTDDRPFGGGPGMVMMIEPIDLALHSLNQKKGTKNKKIILTSAKGRLFTQASARQ
                     WSKLAELTIICGHYEGVDERVAKYLVDAEVRIGDYVLTGGEPAAAVMTDAVVRLLPGV
                     LGNDESGQDESHRTKGLAGPEQYTRPANYRGWSVPEVLLSGDPKKISAYKNQSRNLS"
     gene            3624..4913
                     /locus_tag="PLJ02_02170"
     CDS             3624..4913
                     /locus_tag="PLJ02_02170"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOI05255.1"
                     /translation="MTITIIGTGFVGVVSAAVYASQGNQVIGLDIDPDKINKLKNGQV
                     PFYEPDLKELLIEQQKNHRLTFTTSYQEAISQSEIIIIAVGTPSAPDETADLRYVMKS
                     GDSLAPYLTENAIVAIKSTVPPGTLEKLTEVIKAKSQVKFYGASLPEFLREGSAVYDT
                     LHPSRIVIGAASQFVFDKLDRLHRPFNAPIVHCSVASAQMGKYTANTYLATRITFINE
                     IANLCQKNGADIEEVIQIIGLDQRIGQHYWYPGLGYGGSCFPKDVKELAAYSRTVGEE
                     GNLMNKVNEINKQRIFDLLNNFEEKVDGFANKQVAVLGLSFKPNTDDMREAPSTKVIP
                     FLISKGAQIKSYDPMANYVSFTAIKDEAHEQVASIEEAIKGADAIFLLIEWPEIIKFP
                     YPDYKEAKPQTIFDCRNQLDRTAIKQAGYQYVGIGRS"
     gene            4914..5885
                     /locus_tag="PLJ02_02175"
     CDS             4914..5885
                     /locus_tag="PLJ02_02175"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOI05256.1"
                     /translation="MTNKSQTILITGGTGFAGSHLVEALIEQGYSNIHLTSYRDRPGF
                     LKDLIGDDHIHALDLTDHQATIELFKKLKPQQIYHLASLANVGNSFKQQQFILETHLN
                     LQLNVLNAMKDYCPQARLLSIGSALEYQISNQPLAETAPIGPNDPYALSKMIQDYLSY
                     SYVQSEKLDIVRARPFNHIGERQALGFAVSDFANEIVNIENGHNPTIKVGNLAAVRDL
                     TDVKDMVQAYIILMNQGVRGEVYNIGSGIGYRMEDILKKLIDLAKIKVELVIDPARLR
                     PIDRPVLICDNSKIKNLGWQLQYSIEDTLTRVLNWWRAQAKKTTKER"
     gene            5890..>6167
                     /locus_tag="PLJ02_02180"
     CDS             5890..>6167
                     /locus_tag="PLJ02_02180"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOI05257.1"
                     /translation="MKKAFITGITGQDGSYLAELLLEEGYQVYGLTRRTSTQNFARIQ
                     NIIHHPHLELVSGDLMDQHSLTFAIQKVQPDEVYNLAAQSFVQASWEQP"
BASE COUNT         1877 a         1253 c         1197 g         1840 t
ORIGIN      
        1 cccgaactgg ttgctccctc atttgtcact tatagtattg ctgtctaccc cgatagcgag
       61 gagggtttaa tagttaatcc gccgttatgg caaactcagc aacagtggtt atatatgatt
      121 cgagacttag acaaatttca agccagtctc caaaactttt tttacaatta agggttatta
      181 agcaaactaa caatgatcaa tcaaaaaaat tttaatcgcc tggtcttttt tttactaata
      241 atgttagctt tagctgctgg ctactttcaa tcagccctag aaactcaaga acgtcgctac
      301 caagctcttg atgaacactg ccaaaagtta gaattgatgc tgaccgctag taacagtgcc
      361 caattagaac ttgagtttta agtagaggac taattatgca ggaaattatt tattaccagc
      421 cgcaagagga tttagccctt gctgaaaaca tttatcgcac tgccattgat ggtttgtatt
      481 acgtgagata tcaaaaattt attgatgaaa gaggactttt tgctcaggta cttgagccag
      541 ctcgcattac tcagcagatt aatcctcact ttactattaa acaagttaat ttatctgttt
      601 caaaaactaa agttgtccgc ggtattcatg cagaaaactg gaataaactc attactgtct
      661 taagtggtcg tgctcaattg gtcattgccg atatcaggcc acattcaatt acatataaag
      721 aaactattta ttttgatttt aatgctgata acaaccaaca gtggggtgag gctttataca
      781 ttagtgctca attgggtaat tctatttgtg ccttacaagg gcctgtttat tatctgtatg
      841 gggttgatca actgtatcaa gatcgcaatc aggctgacga tcaggccatt tctctctttg
      901 acccagattt agctattaaa tggccattcc ccaaggaaga aatgattatc tctaagcgag
      961 atttagcaag cattacgtta aaagaactcg aaaaaaagga ctaagatggc aaacactatt
     1021 ttggtaaccg gtggcgcagg ttttatcggc tccaatttta ttcattactg gctcaaacat
     1081 catcctgaag accatttact aaatttggac gccttgacct acgctggtaa tttagctaac
     1141 ttacaaaccg tagctaacca cagtaattac cactttgttc aaggtaatat ttgtgaccgg
     1201 ccattagtta atcaactaat gaaagacgtt gatattgtgg tgcattttgc tgctgaatct
     1261 cacgtagatc gttccattct tgaccctgcc ttgtttctag aaaccaacgt gatcggcact
     1321 caagttttac tggaagcagc gctaaaaaat aagattaagc gttttcacca tatttccact
     1381 gacgaagttt ttggcgcctt accactgact agccaagaaa aatttaataa acatactgcc
     1441 tacgatcctc acagccccta cagtgctagc aaggctgcag ctgatcactt ggtgcgggct
     1501 tatggtgata cttatggttt gcctgtcact attagtaatt gctcaaataa ctatggcgaa
     1561 taccaattcc ccgaaaaaat aattccgtta gccatcacca atttactgga tgggcaaaag
     1621 gtacccattt atggcgacgg gctctatgtc agagactggc tctatgttca agatcactgt
     1681 cgcgggattg atgccgtgct taatcgggga aaagtcggcg aaacttattt atttggtggc
     1741 ttatataaag atatcagcaa tcttgagttg atcaaaatga ttattaagct aatgaatcag
     1801 gacgagagtg cgatagcaat ggtcaaagat cgtcctggcc atgatcgtcg ctatagtgtt
     1861 gatttttccg aaacagaaaa agctcttgat tggcgaccaa gcgttgatct agaaactggt
     1921 ctgaggcgga ctattgagtg gtatcaaact aatgaagctt ggtggcgacc actaaaagaa
     1981 aaaaatcaag cttattttca acaacaatat ggaaagacca atgactaaac agcatatttt
     2041 agggaccggt ttaaatggtt tggttggctc caaatttgtc agcgatttta gtgatcgata
     2101 tctttttgat aatttagatt tgcgagacaa gcaacgccca gtcgatatta cccagcaaca
     2161 acaagtggaa agtgtcatcg ctcaatcagc tgctaaattt ttaattcatt ttgccgcttt
     2221 caccaatgtt actgctgctt ttgcccaaag tggtgataaa aatggcttgt gttatcaagt
     2281 taatgttgtg ggtaccaaaa atattgctca agcttgccaa aagtttaaca aacatttaat
     2341 tcatatttcc actgcctatg tttttaacgg tgaaaaagaa ggtctatata ccgaacaaga
     2401 cccactctcc ccaattgaat ggtatggcca aactaaagct tgggcagaag aagtggtgca
     2461 atctacaccg ggtttaactt ggacgattct ccgcattgat caaccttttc gctcagaccc
     2521 ctttgccaaa ttagatgtcg cttggaaact gattgagggg atgcgggctg gtaaaattta
     2581 tcctcagttt actgatcact attttggtcc aacttacatt gatgatttcg ctaaagtctt
     2641 ggacgctgtc attcgcttaa atttaactgg tttgtatcat gccactagtg gtgaacaatg
     2701 gactgacttt gacttcgccc gcagtctggc acaacaacta catattgatt atcaggtcga
     2761 agccggtagt ttagctaaat atcttgaaac cagtcagcga ccttatcaaa aaaataccgc
     2821 cctttctgtc gacaaattaa agtctgtctt agatttttca ttgactccaa tcaccacagc
     2881 gatgagtcac atcttagctt aaatttcttg attgattttt gtaagcagaa atttttttag
     2941 gatccccaga aagtaaaact tcaggcacac tccagcctcg ataatttgct ggtctggtat
     3001 attgttcagg tccagctaag cccttggtgc ggtgtgattc atcttgacca ctttcatcat
     3061 tacctaacac ccctggtaat aaacgtacta cggcatcagt catcaccgcg gctgcgggtt
     3121 caccaccggt taaaacataa tcaccaatcc gcacttccgc atcaaccaaa tatttagcta
     3181 ctcgttcatc aactccctca taatggccac aaatgatggt taattctgct aatttactcc
     3241 actgtcgagc acttgcttgg gtaaagagtc gacccttagc cgaagttaaa atgatttttt
     3301 tgttttttgt tccctttttc tgattaaggc tgtgcagcgc taaatcaatt ggttcaatca
     3361 tcattaccat ccctggacca ccaccaaacg gtcgatcatc ggtagtgtgg tgcttatcct
     3421 gggcaaaatc acgaagatta ataacttgat atttgaccag ctgtttttgt tgagctcttt
     3481 tcagaataga agagtcgaga atattactaa aatattcagg aaaaatagtc aaaaaattga
     3541 tctgcatagc tttctcttac tggtgttaac tgccgctaat cattataata caaatgtaaa
     3601 aaatacgagg aatccgaaga aatatgacaa tcaccattat tggcaccggt ttcgtaggag
     3661 tggtttcggc agcagtttat gcttctcaag gcaatcaagt tatcggttta gatatcgatc
     3721 cagacaaaat taataagcta aaaaatgggc aagtcccttt ttacgaacca gatttaaaag
     3781 aactgctaat cgaacagcaa aaaaatcatc gattaacttt taccacttct tatcaggaag
     3841 cgattagtca gagtgagatc attatcattg ctgttggtac tccctctgct ccagacgaaa
     3901 ctgccgactt gcgttatgtg atgaaaagtg gtgacagttt agctccttat ttgacagaaa
     3961 atgcgatcgt ggccattaaa tccaccgtcc cacctggtac actggaaaaa ttaactgaag
     4021 taataaaagc caagagccaa gttaagttct atggagcgtc cctacctgaa tttcttcgtg
     4081 aaggcagtgc tgtttacgat acgcttcatc ccagtcggat tgttatcgga gcagctagcc
     4141 agtttgtctt tgataaatta gatcggctcc accgtccttt taatgcccca atcgttcatt
     4201 gcagtgtggc ttctgcgcaa atgggcaaat acaccgctaa tacctattta gctactcgca
     4261 tcacttttat taatgaaatt gccaacctct gtcaaaagaa tggggccgac attgaggaag
     4321 tcattcaaat tattggccta gatcaaagaa ttggccaaca ttattggtat ccaggcttag
     4381 gctatggggg ctcttgtttc cctaaggatg ttaaagaact agctgcctac tctagaactg
     4441 ttggtgaaga gggcaactta atgaacaagg tcaatgaaat taataaacaa cgaatttttg
     4501 atttattaaa taattttgaa gaaaaagttg acggttttgc taacaaacaa gtggcagtgc
     4561 ttggtttatc tttcaaaccc aataccgacg acatgcggga agcaccgagt accaaagtta
     4621 tcccctttct gattagtaaa ggcgctcaga tcaaaagcta tgatcccatg gctaactatg
     4681 tctctttcac tgcaatcaaa gatgaggcgc atgagcaagt cgcaagtatt gaagaagcca
     4741 tcaaaggtgc tgacgcaatt ttcttattaa tcgagtggcc agagattatt aaatttcctt
     4801 accctgatta taaagaagct aagccacaaa ctatttttga ctgtcgcaac cagttggacc
     4861 gaacagccat caaacaagct ggttatcaat atgtagggat tggaagatct taaatgacca
     4921 ataaaagtca aactatttta atcaccggtg gcaccggctt cgctggcagt cacttagttg
     4981 aagctctaat tgaacaaggt tatagcaata ttcacttaac atcttatcga gatcgacctg
     5041 gttttcttaa agatctcatt ggtgacgatc acattcatgc gctcgattta actgatcatc
     5101 aggcaactat tgaactattc aaaaaattaa aaccacagca aatttatcat ctggcctcgc
     5161 tagccaatgt tggcaacagc tttaaacaac aacaatttat tttagaaact catttaaatc
     5221 tgcaattaaa tgtccttaat gcgatgaagg actactgtcc tcaagcccgc ttgttaagta
     5281 ttggctccgc gttggaatat caaattagta atcagccctt ggctgaaact gctccaattg
     5341 ggcccaatga tccttatgcc ctttccaaaa tgatccaaga ttatttaagc tattcctatg
     5401 ttcagagcga aaaattagat attgtccgag cacgaccgtt taatcatatt ggtgaaagac
     5461 aggcgctggg ttttgctgtc agtgactttg ccaatgaaat cgttaacatt gaaaacgggc
     5521 acaaccccac tattaaagta ggcaatttag ctgcagtaag agatttaacc gacgtgaaag
     5581 atatggttca agcctatatt attttaatga accagggagt gcgcggtgaa gtttacaata
     5641 ttgggtcagg aattggttac cggatggaag atattttaaa aaagctgatt gatttagcta
     5701 aaattaaagt ggaactggta attgatccag cgcgtctacg accgattgat cgacccgtat
     5761 taatctgtga taatagtaag atcaaaaact taggttggca gcttcaatat tcaatcgaag
     5821 acactttaac cagagtactc aattggtggc gtgctcaagc caaaaaaacg actaaggaaa
     5881 ggtaaaaaca tgaaaaaagc ttttattact ggcattaccg gccaagatgg ttcttatttg
     5941 gccgaattat tgctggaaga aggttatcaa gtttatggct taactcgtcg cactagtact
     6001 caaaattttg cgcgtattca aaatattatc catcatcccc atttagaatt agtttctggt
     6061 gatttgatgg atcaacattc acttactttt gccatccaaa aggttcaacc tgatgaagtt
     6121 tataatttag cggcacagtc attcgtccaa gcttcttggg aacagcc
//
LOCUS       DAOSEW010000043         6153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_11231_length_6153_cov_3.903903, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000043 DAOSEW010000000
VERSION     DAOSEW010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 6153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6153
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11231_length_6153_cov_3.903903"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PLJ02_02185"
     CDS             complement(<1..296)
                     /locus_tag="PLJ02_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOI05258.1"
                     /translation="MKITVLQENLKQQLDYLQKVIPSKPQLPILSSILLRIEKNKLLL
                     AATDLYLGIRASLVVEVKEEGSLVVDGDTFRSLIASLSPGKIDLELSEAVLKIT"
     gene            complement(700..2217)
                     /gene="dnaA"
                     /locus_tag="PLJ02_02190"
     CDS             complement(700..2217)
                     /gene="dnaA"
                     /locus_tag="PLJ02_02190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOI05259.1"
                     /translation="MSEQSYDNQTIEQIWQMAKNDLKSSLSPAIFNTWILPSPLTQIR
                     IIDELKAEETISCMTAFHATNLKSNLYLPIKTALEKYVNRPLELNFVVGTNKHTAQPT
                     NGFTKTDQRFDQRQEEDGKPATTLENGDRGELLSPTVDELFSYSNIKNNTVDRYLLRA
                     KQIGLNIDYTFKNFAVDPSNQMAHAAATKVASSLGTSYNPLFLYGGVGVGKTHLMHAI
                     ANYVLKNEPDTNIVFCTGEEFTNEIIRSIQNKNSAQFRNKYRHANLLLLDDVQFIAGK
                     KSVQEEFFHTFNALTANNSQIVLTSDLPPHEISLLEDRIRSRFEAGLMIDIQQPTLEL
                     RTAILLLKAQSNHINIPNHLAQRIAAQVDSARRIEGIIKKIRSAVELQGAEINVELID
                     LILKKEIRQNHQKTLRVKPQDVIQSVSNHFHLKQSAIKGGRRNKELVRARHIAMYLLR
                     QELNCSLEEIGQWFGGRDHTSVIHAVNKIEQDILVDETVAQDVSALKMSLVAISR"
     gene            complement(2315..4489)
                     /locus_tag="PLJ02_02195"
     CDS             complement(2315..4489)
                     /locus_tag="PLJ02_02195"
                     /inference="COORDINATES: protein motif:HMM:NF040179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI05260.1"
                     /translation="MPSFNLDLPFIKKKKPAVDPVAQKQIDAEQYTQEKLRDFSRGLV
                     TIQDIIAPEAIEVDFTYQKINSTYTRTLFVAGYPRSIPANWLSPLINFPAQANISMFI
                     FPTDTSEVLDNLKRKITEMEAEITSDVRSGKISNINTEIKLNDARVMREQLAAGSERF
                     FQFALYITIKSESKEQLEKITKAIQSSLGSLLIVSKKAALQMDEGFKTTLPMGVDRLL
                     ITRNMDTTSLATTFPFTSSELTMETGIVYGINEHNDSLVIFDRFKMENANMVIFAKSG
                     AGKSYTVKLEILRQLMFDTEIIVLDPEHEYEELCKMVNGEYINFTFGSQAKINPFDLS
                     NLYEEGENELGQKILSLHGLLKVMLGEMTPQQEAILDRAIIASYKAKGITPDPNTQTN
                     TPPLMEDLYKALVGMEQDESDDLAARLEKFITGSFRGIFDKPSNVDIQSPFTVFSVKE
                     MEEELRPIAMYVILDFIWTRVKKDLKKRILVIDEAWYFMMHQDSSAFVYSMVKRARKY
                     YLGITTITQDVEDFLRNDYGRAIISNSSIQFLMKQATSAIPLLAQTFYLSEGERQLLV
                     SSDVGEGIFFAGQNHVALRVIASEKEHQAITTDPEEILAQRRQAALNRELRRRRLEQE
                     NKADTGDQKIGSFEKPEKIPWQGEPVDISQDVLEDAERKAENSLKQNPVLPQIKSIFD
                     AEATTTPTNTESADNNPDQNTDKAPEKNQHFSYSIDDYQAPK"
     gene            complement(4490..5644)
                     /locus_tag="PLJ02_02200"
     CDS             complement(4490..5644)
                     /locus_tag="PLJ02_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05261.1"
                     /translation="MSDNTISSTTQEYLDVYDITNDMVILKDGVVSIIMQIGTMNFSL
                     LAEQEQDAIMYTYGALLNSLNFPIQINIQSTIKDASRYLRLLDNQIKNSESRAKAQLI
                     GEYRDFVANLIRERNVLEKKFFVVIPTNAAEMGLYTADSVLPGKTKFDINNFEKTVLI
                     EKAATILDPRRDHLISQFNRIGLFARQLDTQEIIQNFYINYNPESMEGQEVTNSESYT
                     TPIVRASISSEQNLDQLRQEFKQTINDQPAQHNQLGQEANQEALEMGQEPTGLQGVQE
                     TPEEKNIEVQQVPTEKIAEVQEAPAPKTVEPQGENSNYALKTNQAQQPTVSAIPNFSE
                     EIIPLQARAINEQPAHHQATDDFALKSNQVPSPVELKQKPINNNPNPVEL"
     gene            complement(5726..>6153)
                     /locus_tag="PLJ02_02205"
     CDS             complement(5726..>6153)
                     /locus_tag="PLJ02_02205"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOI05262.1"
                     /translation="RIRKLKSLEDIQEQQTPKMELESTSKEPDSLNQSATVKTEANQS
                     HNTEQPPIKEEKNNQLQGTIKDSSGQAVSNALIEVFAKNEDQALRISKSNANGEFFIK
                     TALPNGEYQIKTEAEGLVFMPISIRLTGRAVPTIEIVAE"
BASE COUNT         1712 a         1124 c         1201 g         2116 t
ORIGIN      
        1 tgagtaattt ttaaaacagc ttcgcttaat tctagatcaa tctttccagg agagagtgaa
       61 gcgattaggc tacgaaaagt atccccgtca acgaccaatg atccctcttc tttgacttcg
      121 accaccagac tcgctcttat ccctaaataa agatcagtgg ctgctagaag caatttgttt
      181 ttttcaattc tgagcaagat tgaagataaa atcggcaact gtggtttact gggaatcacc
      241 ttttgcaaat aatccaattg ttgttttaag ttttcttgta agactgtaat tttcataaat
      301 acttttttta ttgtaaacga taagtttaat tctaaccaat ggttttactt tttttttaca
      361 ttttactaat ttaactttac ttagtaacag tagtagtagg gggtgtgaaa aagtgaagaa
      421 aacataagtt tttccgtaaa gaacgaagca gacgcgcctt tttttactaa ttcttattgt
      481 ggaaaagtcg aagaaatttg tagatttttt tgttgaatat tgcgcatatt ttggtaaaaa
      541 ttactctagt aatcaacatt caaccaaaag ttattcacga ttattcacca cttattcagg
      601 ttttttccac aaagttatgc acttttctga ataacttatt gaaacaagac ccaaaaaaaa
      661 taaaaaaagc ccaaccatca gttttttaat gtgctaaaac tacctagaaa ttgctaccaa
      721 agacattttg agagcagaaa cgtcttgcgc aacggtttca tcaactaaaa tatcttgttc
      781 aatcttattg acagcgtgaa tgacactggt gtgatctcta ccaccaaacc attgaccaat
      841 ttcttccaaa gagcaattca attcttgcct caacagatac atggcgatgt gtcttgctct
      901 aaccaactct ttattgcgcc tgcctccctt aatagcgctt tgtttaaggt gaaagtgatt
      961 gctgacgctc tgaatcacgt cttggggctt aacccgtaaa gttttttggt ggttttgccg
     1021 aatttctttc tttaaaatca aatcaattaa ttcgacatta atttcggctc cttgtaattc
     1081 aacggcactg cgaatttttt taataatccc ctcaattcta cgagcactat caacttgagc
     1141 ggcaattctc tgggccaagt gattgggaat attaatgtga tttgattgag ctttaagtaa
     1201 caaaatggcg gtgcgcaatt caagcgtggg ctgttgaata tcaatcatta aacctgcctc
     1261 aaaacgagag cggattctat cttccaaaag actaatctca tgaggtggta aatctgaggt
     1321 taaaacaatt tgagaattat tagcagttaa agcattgaaa gtatgaaaaa attcctcttg
     1381 cacgcttttt ttgccagcaa taaattgcac atcgtccagt aataataaat tcgcgtggcg
     1441 gtatttattg cgaaattggg ctgagttttt gttttgaata gagcgaataa tttcattagt
     1501 aaactcttcg ccagtgcaaa aaacgatgtt agtatctggt tcgtttttta acacgtaatt
     1561 agcgatggca tgcatcaggt gagttttgcc cactccaacc cctccatata gaaatagggg
     1621 attataacta gtaccaagac tgctggccac tttggtggca gcagcatggg ccatttgatt
     1681 actgggatca acggcaaagt ttttaaaagt atagtcgata ttaaggccaa tttgcttcgc
     1741 tcttaaaaga tacctatcta cagtgttatt tttgatatta gagtaagaaa atagttcatc
     1801 tacagttggg gagagtaatt cgcccctatc gccattttct agcgtggtgg cgggtttacc
     1861 atcttcttct tgtctttggt caaagcgctg gtcggttttt gtaaagccat tggttggttg
     1921 ggcggtgtgt ttgttggttc cgacaacaaa atttaattct aatggccgat taacgtattt
     1981 ttccagagcg gttttaattg gtaaataaag attggatttt aagttggtgg catgaaaagc
     2041 ggtcatacaa ctaattgtct cctcggcttt taactcatca ataattctaa tttgagttag
     2101 agggctgggt aaaatccaag tattaaagat ggcaggactt aagctacttt ttagatcatt
     2161 tttagccatt tgccaaattt gttctatagt ttgattgtcg taactttgtt cagacataaa
     2221 ttgagccaaa acgttcgggc agaattaaat atgcaaaaga aaaattgcaa gcacagttat
     2281 acacagagtt ttccacattt gacaaggggc aattttattt aggggcttga tagtcatcaa
     2341 tagaatagct aaagtgttga tttttttctg gggctttatc ggtgttttga tctggattgt
     2401 tgtcagcgct ttcagtgttg gtgggtgttg ttgtagcttc agcatcaaaa atacttttta
     2461 tttggggcaa aaccggattt tgtttcaaac tattttccgc ttttctttca gcatcctcta
     2521 gtacatcctg actgatgtcg actggctcgc cctgccaagg aattttttct ggtttttcaa
     2581 agctcccaat tttttggtcg ccggtatcag ccttattttc ttgttctagt cttctgcgtc
     2641 tcagttcacg atttaaagca gcctgtcttc tctgagccaa tatttcttct ggatctgtgg
     2701 taatggcttg atgttctttt tctgaggcga tgacccgaag tgcgacgtga ttttgcccgg
     2761 caaaaaagat gccttctccc acgtctgaag aaaccaacaa ttggcgctca ccttctgaca
     2821 aatagaaagt ttgggccaaa aggggaatcg ctgaggttgc ttgtttcatt aagaactgga
     2881 tagaagagtt agaaataata gcccggccat aatcattgcg caaaaaatcc tctacatctt
     2941 gagtaatggt agtgatgccc aaataatatt tgcgcgctct tttgaccatt gaataaacaa
     3001 aagcagagct atcctggtgc atcataaaat accaagcctc atcaatgact aaaatgcgtt
     3061 ttttaagatc ttttttaact cttgtccaga tgaaatcaag aataacgtac atggcaatag
     3121 gtcttaattc ttcttccatt tctttaacgc taaataccgt aaaaggagat tgaatatcaa
     3181 cgttagatgg cttatcaaaa atcccgcgaa aagaaccagt aataaacttt tctaagcggg
     3241 ccgccaaatc atcactttcg tcttgttcca tgccaaccaa ggctttgtaa agatcttcca
     3301 ttaggggtgg ggtattggtt tgtgtgttgg gatctggggt aatgccttta gctttgtaac
     3361 tggcaataat tgctcgatct agtatggctt cttgttgtgg ggtcatttca cccaacatca
     3421 cttttaacag accgtgaagt gagagaattt tttggccaag ttcgttttca ccttcttcat
     3481 aaagattaga taaatcaaaa ggattgattt tagcttgaga gccgaaggta aagttaatat
     3541 attcgccatt aaccatctta cataattcct cgtactcgtg ttcggggtct aaaacaataa
     3601 tttcggtgtc aaacatgagt tgtctcaaaa tttctaattt gacggtataa gatttaccag
     3661 cgccagattt agcaaaaatt accatattgg cattttccat cttaaaacga tcaaaaatga
     3721 ccagtgaatc attatgttca ttaatcccat aaacaatccc tgtttccatc gttaactcgg
     3781 aagaagtaaa ggggaaagtg gtggccagcg aagtggtatc catatttctc gttataagca
     3841 ggcgatcaac acccataggc aaagtagttt taaagccttc atccatttgt aacgctgctt
     3901 ttttggacac aattaagagg gatcctaaag aagattgaat agccttagtg attttttcta
     3961 gttgttcttt actttcactt ttaatagtta tatagagagc aaactggaaa aacctttcag
     4021 atcctgccgc taattgctca cgcatgaccc gcgcatcatt aagtttaatt tcagtattaa
     4081 tgttgctaat ttttcccgat ctaacgtcag aggtaatttc tgcctccatt tcagtgattt
     4141 ttctctttaa attatctaga acctcactgg tgtccgttgg aaaaataaac atcgaaatat
     4201 tggcttgagc tgggaaatta attagaggtg aaagccagtt ggcggggata cttcggggat
     4261 aaccggccac aaacaaagtt ctagtataag ttgaattaat tttttgataa gtaaaatcta
     4321 cttcaatcgc ttctggagca ataatatctt ggatggtcac taagccacga gaaaaatcgc
     4381 gcaatttttc ttgagtgtat tgctcggcat caatttgttt ttgcgcaaca ggatcaacag
     4441 ctggcttttt tttctttatg aatggtagat ctaaattgaa tgatggcatt taaagttcaa
     4501 cgggattggg attattatta ataggttttt gttttaattc caccgggctt ggaacttgat
     4561 ttgattttag tgcaaaatca tcagtagcct gatgatgagc tggttgttca tttatggccc
     4621 ttgcttggag cgggataatt tcctcactaa aattggggat agctgaaact gttggctgtt
     4681 gagcctgatt tgttttaagc gcgtagttgg agttttctcc ttgaggttca actgtttttg
     4741 gtgctggtgc ttcttgtact tctgcaatct tctctgttgg cacttgttgt acttctatat
     4801 ttttctcttc tggcgtttct tgaactcctt gtagccctgt aggttcttga cccatctcaa
     4861 gcgcctcctg atttgcttct tggccaagct gattatgctg tgcgggctga tcgtttatgg
     4921 tttgtttaaa ctcttgacga agttgatcga gattttgttc actactgata ctggctctaa
     4981 ctattggcgt ggtatagctt tcactattgg taacttcttg accttccatt gattctggat
     5041 tgtagttaat ataaaaattt tggattattt cctgagtatc gagttgtcta gcaaataaac
     5101 cgatgcgatt gaattggcta attaaatggt ctcttcttgg atcaaggatg gtggcggcct
     5161 tttctattag tacggttttt tcaaaattat taatatcaaa cttggttttt ccaggcaaaa
     5221 cgctgtcagc agtgtagaga cccatttcag cagcgttagt aggaatcacc acaaagaact
     5281 ttttttccag aacattacgt tcacgaatca aattagcaac aaaatctctg tattcgccaa
     5341 ttaattgagc ttttgctctt gattcgctat ttttaatctg attgtcgagc agtcttagat
     5401 atcggcttgc atctttaatg gttgattgga tattaatttg aataggaaaa ttgagggagt
     5461 ttaacagtgc cccataagta tacataatgg catcttgttc ctgctcagct agcaggctga
     5521 agttcatggt accaatttgc ataattatgg aaaccacacc gtctttgagg attaccatat
     5581 cattagtgat gtcatagaca tcaagatatt cttgggtggt agaactaatc gtgttatcgc
     5641 tcatgagctg tatttaagtt tactagattt tgactgttaa taaaagaccg ttaataaaag
     5701 accgttaata aaaaaaacct tgatcttatt cggccacaat ttcaattgta ggaaccgcac
     5761 gacctgttag tctgatggaa attggcataa aaactaaacc ctctgcctcg gttttgattt
     5821 gatattcccc gttgggaaga gcagtcttga taaaaaactc tccattggcg ttgcttttac
     5881 taattcgtag cgcttgatct tcatttttcg caaacacctc aattaaggcg ttactaacag
     5941 cctgaccact actatctttt attgtgcctt gcaattgatt gtttttttct tcttttattg
     6001 gtggttgttc ggtgttgtga gactgattcg cttccgtttt gacagttgcg gactggttca
     6061 aactatctgg ttctttgctt gttgattcca actccatctt tggtgtttgt tgttcttgaa
     6121 tgtcttctaa acttttaagt tttctaattc tgg
//
LOCUS       DAOSEW010000044         6140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_11265_length_6140_cov_4.144782, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000044 DAOSEW010000000
VERSION     DAOSEW010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 6140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6140
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11265_length_6140_cov_4.144782"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PLJ02_02210"
     CDS             <1..197
                     /locus_tag="PLJ02_02210"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990676.1"
                     /note="catalyzes the formation of glycerone phosphate and
                     D-glyceraldehyde 3-phosphate from D-fructose
                     1,6-bisphosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HOI05263.1"
                     /translation="GESATEADFTKDLKAAVRSAVINKRAGGAGLILGRKAFKRPMEE
                     GVKLLRAVQDVYLEEQITIA"
     gene            complement(235..840)
                     /gene="rdgB"
                     /locus_tag="PLJ02_02215"
     CDS             complement(235..840)
                     /gene="rdgB"
                     /locus_tag="PLJ02_02215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOI05264.1"
                     /translation="MQLLFVSQNAGKIAEAQRIFLHSRHQLITLDQKSWLKKQGITIP
                     DNFVVAESGSTFRENALLKAQAFHQLTKLPCIADDSGLLLAAFPNFPGVNSHRWFNGS
                     DAERNLALLRKLAQVENRRAKFQTVLCLFAFKQEPLYFSGEVEGTIAFTSLGQEGFGY
                     DPIFIPDGYQKTFAQLGVKVKNTISHRALAWQSLFSYLENS"
     gene            complement(845..1132)
                     /locus_tag="PLJ02_02220"
     CDS             complement(845..1132)
                     /locus_tag="PLJ02_02220"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOI05265.1"
                     /translation="MADKAGLLGQLGDLNKLRKQAKQMQKELASEEIVVEEGDIVVTI
                     AGDQKIKDVAVQGIHSADLVNALNKAIKKSQEVAAKKLQTMTGGLGGLMGR"
     gene            complement(1177..2733)
                     /gene="dnaX"
                     /locus_tag="PLJ02_02225"
     CDS             complement(1177..2733)
                     /gene="dnaX"
                     /locus_tag="PLJ02_02225"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOI05266.1"
                     /translation="MAWYRQYRPKTIAELDIVQVRESLQAMMRAGKIPQSLIFAGPKG
                     TGKTSASRILAVLLNDPKNEALVDQLYFGKQSKSKKPLFFQEPDPNSELAQKVYQGQS
                     YLVHELDAASNRGIDDIRLLKERIALPPQGAKMAVYILDEVHMLTPEAFNALLKILEE
                     PPPHVVFILATTELHKLPATIISRCTLLNFHKATEEEIVASLERILQAEKIDYQIEDL
                     KKIAHRADGSFRDAVKNLELACQSGKLTIDAISAYLGQDNITYIKELLTLLLKKDAKT
                     IAERFEELRAKNYQEQDFYKDLLEFLHDDLLINLGIRPGEAFTESKIDQFLLKEFSQN
                     NLQQNLPINFLALELIFLGIIDRSNEQGKKTNNTQQHSNNLPSSEEKTGKNSKKKIEL
                     VEKSLNSYHTESISQQESTNIENSPLSKISAKELWQKLLDFVAKENFSLSALLRACKL
                     EEIIDGTARIIVFYDFHKEQLEQQKYQTLLDESFQNLLGEKLKFDFILSKAPVTEDSD
                     LLETAKDVLI"
     gene            complement(2765..3160)
                     /gene="ybeY"
                     /locus_tag="PLJ02_02230"
     CDS             complement(2765..3160)
                     /gene="ybeY"
                     /locus_tag="PLJ02_02230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOI05267.1"
                     /translation="MIKSNLYIGSRYPVKRKKLRETVKRVLKNHGIDNAQVDISIVGS
                     RKIKQLNEEKLGHFGTTDVLSFPQHDKDQLQDVPLPEELAPHLGDVVISFPEAVKTAR
                     RFGRLVDDQINFYLEHGLMHLLGYHHDDH"
     gene            complement(3169..3990)
                     /locus_tag="PLJ02_02235"
     CDS             complement(3169..3990)
                     /locus_tag="PLJ02_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05268.1"
                     /translation="MDKKKNTTLKNSSQQLVAGLAMAGQMAMMNPQIAKEVITDKAIY
                     AKAGQKDNDDLALDLSATEYQEIMAKLNSHVNGPAGHLETETQLYLEQQLSLILGFDV
                     RHELDGKKLNHSIGIMGAEQHLMRYPGDSLAQHDAYQEAGIAPKRGAFSWFTEAGELT
                     EKAILREKYYFAVQLMYLPNWNQDYPELKPWYKYRKMIMINPSEQVAVVGVVADAGPA
                     AWVQKQFGGSPEVIREGRVWSNNSRGRVMLLFVDDPDDKIPLGPIDISFAALISA"
     gene            complement(3992..5455)
                     /gene="gltX"
                     /locus_tag="PLJ02_02240"
     CDS             complement(3992..5455)
                     /gene="gltX"
                     /locus_tag="PLJ02_02240"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOI05269.1"
                     /translation="MQDKQLPIRTRMAPSPTGEMHVASMATLLKNYAFAKRHHGQFIL
                     RIEDTDKTREVPNGVEQIQAIIRKFGLDWDEGPGKDGPYGPYIQSQRINIYQEKAQEL
                     VAKGQAYYCFCSKQRLEEVRQQQQANHQLPRYDQHCRYLSEEEVQKKLAAGESYVIRL
                     KVPANQDIVFTDLLRGEISFNSNLVDDQVLLKSDGYPTYHLAVVVDDHLMKISHVMRG
                     EEWISSTPKHVLLYQAFGWDLPIFAHFPLFLNPDGKGKMSKRKGTVSAQSFLDRGYLP
                     EALLNFLMILGWARKDQKEIMSLEEYIEAFDPRDLSVNSVVFDVKKLDWLNGIYIRKL
                     DRPELKKRLQPFIPPAFPHQKIDSILDLVYERLVTLADFAELTDFFYKEIDYSTTTLL
                     KKAQDQPGLVDEQIQETKRVIASLDVVDANSLEKCIRQLQEKHDWHKGQYFMMLRIAM
                     TGRTATPPLFETMAVLGKDLILERLDLALKRLSENNK"
     gene            complement(5458..>6140)
                     /locus_tag="PLJ02_02245"
     CDS             complement(5458..>6140)
                     /locus_tag="PLJ02_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05270.1"
                     /translation="RFTKTQPLDTSLEINATYLNFSISSNYLAKPELLITDNTKRLPE
                     EFEAVERVKNFLIEGGLMPKDVATSSGVISYKKSLGGELTPALSLSDADFVTVDLNRV
                     PIDDYYPIYTPAGEKGVISAVISGAFTGSNSIVEVDYYYHEVDYSTFETYPLRGVKSA
                     WKMVQAGEGYIASGQNLERAVIRSVELAYFDDFEYQPFLQPIYVFKGDDNFLAYVSAI
                     HPNYLERE"
BASE COUNT         1847 a         1273 c         1034 g         1986 t
ORIGIN      
        1 gtggggaaag tgctaccgaa gctgatttta caaaagattt aaaagctgcc gtcagaagtg
       61 cggtgattaa taaacgagct ggcggtgctg gactgatctt aggcagaaaa gccttcaagc
      121 gccccatgga agaaggtgtt aaattactaa gagctgtcca agatgtttat ctagaagagc
      181 agattactat tgcttaaata tcgtccagat attgtcgaaa tattagctaa ttatttaact
      241 attttccaaa taactaaaca gtgattgcca agccaaagcg cgatgagaaa tggtattttt
      301 gactttaact cccaattgag caaatgtttt ttgatagcca tcgggaataa aaattggatc
      361 gtaaccaaaa ccttcttgac ctagactggt aaaagcgatg gtcccctcca cttctcccga
      421 aaaatagagc ggttcttgtt taaatgcaaa caaacacaag acagtttgga acttagcccg
      481 acgattttct acctgagcca attttcgcaa aagagctaaa tttcgctcgg catcacttcc
      541 gttaaaccag cgatgggaat tcacacctgg aaaattagga aaagcagcca atagtaaacc
      601 actgtcatcg gcgatgcagg gtaatttcgt caattgatga aaagcttgag ctttaagtaa
      661 agcattttct ctaaaagtgc tacctgattc tgccacgaca aaattatcag gaatagtgat
      721 tccttgtttt ttaagccaac ttttttgatc cagcgtaatt aactgatgac gggagtgaag
      781 aaaaattctt tgagcctcag caattttacc tgcattttga ctaacaaaga gtagctgcat
      841 actactatct acccattaag ccacccaaac caccagtcat ggtctgcaat ttcttagcag
      901 ccacttcttg agattttttg atggctttgt tcaaagcgtt aaccaaatca gcactgtgaa
      961 taccctgaac agcaacatct ttaatttttt gatcaccagc tatagtcacc acaatgtccc
     1021 cttcctcaac tacaatttcc tcactggcta attccttttg catttgctta gcctgtttac
     1081 gcaatttgtt aagatcacct aactgaccta ataaaccagc tttgtctgcc atatttgtcc
     1141 tttttaataa ttgttaataa ttagctgtaa actgaattat atcaaaacat cttttgccgt
     1201 ctctagcaaa tcactgtctt ctgttactgg tgccttactg agaataaaat caaattttaa
     1261 tttttcaccc agtaaattct ggaaactctc atctaacaag gtttgatatt tttgctgttc
     1321 caactgttct ttgtgaaaat catagaaaac gataattcgg gctgtaccat ctatgatttc
     1381 ttctaattta caggctctta gcaaggcgga caacgaaaaa ttttccttgg ccacaaagtc
     1441 tagtaatttt tgccaaagtt ctttagcaga aatttttgat aatggagaat tttcaatatt
     1501 tgtcgattct tgctgactta tagactcagt gtggtagctg tttaaacttt tttctaccag
     1561 ttcaattttt tttttgctgt ttttaccagt tttttcttcg ctagaaggca aattatttga
     1621 atgctgctgc gtattatttg ttttctttcc ctgttcattg gaacgatcaa taatccccaa
     1681 aaaaattaac tctaaagcta aaaaattaat tggcaaattt tgttgtaagt tattttgtga
     1741 gaattctttg agtaaaaatt ggtcaatttt actttcagta aaagcctcac ccggcctaat
     1801 tcccagatta attaataaat cgtcgtgcaa aaattctagc aaatctttat aaaaatcttg
     1861 ttcttgatag ttttttgctc ttaattcttc aaatctttcc gcaatagttt tagcatcttt
     1921 cttcaacaat aaagttaaaa gttctttaat ataggtaata ttatcttgtc ccaaataagc
     1981 gctgatggcg tctattgtca attttccact ttgacaggct aattctaaat ttttgactgc
     2041 atctctaaaa ctaccatcag ctcgatgagc aatttttttt aaatcttcaa tctgataatc
     2101 aattttttct gcctggagta tcctttcgag tgaagctact atttcttctt ctgttgcttt
     2161 atgaaagtta agtaaagtgc aacgagaaat gatagtagca ggcaatttat gcaactcggt
     2221 agtggctaaa ataaacacta catgtggggg tggttcttct aaaattttta acaaagcgtt
     2281 aaatgcttca ggagtgagca tgtgtacctc atccaaaata taaacagcca ttttggcacc
     2341 ctgagggggt aaagcaatgc gctctttgag cagtcgaata tcatcaatac cacgattact
     2401 agccgcatct aactcatgaa ctaaataaga ttgaccttga tatacttttt gggctaattc
     2461 cgaattggga tcgggttctt gaaaaaataa cggcttttta cttttggact gtttgccaaa
     2521 gtatagttga tcaactaatg cttcattttt gggatcattc agtaaaacag ccaaaattct
     2581 tgaagctgaa gttttacctg taccttttgg cccagcaaaa atcaacgact gtggaatttt
     2641 gccagccctc atcatcgctt gcaagctttc tctcacttgc acaatatcaa gttctgcaat
     2701 agtttttgga cgatactgac gataccaagc cataatactt ttttagttta acatattaaa
     2761 aaaattaatg gtcatcatga tgataaccta agagatgcat gagcccgtgt tctaagtaaa
     2821 aattgatttg atcatcaacc aaacgaccaa atctgcgagc cgttttgacc gcctcaggaa
     2881 aactaatcac tacatccccc aaatgaggag ctaattcttc aggaagtgga acatcttgga
     2941 gctgatcctt gtcatgctga ggaaaagata agacgtcagt tgtcccaaaa tgaccgagtt
     3001 tttcctcatt gagttgctta attttgcgac tgccaacgat ggaaatgtca acctgggcgt
     3061 tatcaattcc atgatttttt aaaacgcgtt tgactgtttc tcgcaatttt ttacgtttaa
     3121 ctggataacg agacccaata tataaatttg atttaatcat agttttcctt aggcagaaat
     3181 tagcgctgca aagcttatat caattggccc taaaggtatt ttatcatcgg gatcatctac
     3241 aaacagtaac attacccgac cccgactatt attggaccaa accctgccct ctcgaatgac
     3301 ttcgggagaa ccgccaaatt gtttttgcac ccaagctgct ggaccagcat cagccactac
     3361 tcctactacc gctacttgtt cactaggatt tatcataatc atttttcggt acttatacca
     3421 aggtttgagc tcgggatagt cttgattcca attaggtaag tacattaact gtaccgcaaa
     3481 ataatatttt tcccgtaaaa tcgctttttc agttagttca ccagcctcag taaaccaaga
     3541 aaaagcgcct cttttgggag caatcccagc ctcttgatag gcatcatgct gagctaaact
     3601 atcgcccgga tagcgcatta agtgttgctc tgcccccata atcccaatac tatgatttaa
     3661 ttttttgccg tctaattcgt gccgaacatc aaaaccaaga attaaactaa gttgttgttc
     3721 taaataaagc tgtgtctcag tttctaggtg tcctgctggt ccattaacat gcgagtttaa
     3781 tttggccata atctcttggt actcggtagc agataaatcc aaagctaagt catcattgtc
     3841 tttttggcca gctttagcat aaatcgcttt atcagtaatg acctctttgg caatttgcgg
     3901 attcatcatt gccatttgac ctgccatagc taaaccagcc actagttgct gagaactatt
     3961 tttaagagtg gtattttttt tcttatccat gttatttatt gttttcgctc aatcttttca
     4021 aggcaagatc tagtctttca agaattaaat ctttccctaa aacggccatc gtttcaaata
     4081 atggcggtgt cgccgttctg ccggtcatcg caatgcgcaa catcataaaa tactggcctt
     4141 tatgccaatc atgtttttct tgcagttgcc gaatacattt ttccaggcta ttggcatcaa
     4201 caacatctaa agaggcaata actcttttag tttcttgaat ttgttcatca actaaaccag
     4261 gctgatcttg agcttttttt agtaaagttg tggttgaata atcaatctct ttgtagaaaa
     4321 aatccgtcaa ttctgcaaaa tccgccaggg ttactaaccg ttcataaacc aaatctaaaa
     4381 ttgagtctat tttttgatgc ggaaatgctg gtggaataaa tggttgcagt cgcttcttta
     4441 attctggtcg atctaatttt ctaatataaa tgccgtttaa ccaatcgagt tttttgacat
     4501 caaaaacaac cgaattaaca ctcaagtctc tgggatcgaa tgcttcaatg tactcctcca
     4561 agctcatgat ttctttttga tctttccgcg cccaacccaa aatcatcaga aaatttaaaa
     4621 gcgcttctgg taaataacca cgatccaaaa aagattgagc cgaaaccgtg cctttacgtt
     4681 tactcatctt gcctttgcca tcagggttta aaaatagcgg aaaatgagca aagattggta
     4741 aatcccaacc aaaggcctga taaagtaaca cgtgtttagg agtggaacta atccattctt
     4801 cacctctcat cacatgacta attttcatta aatggtcgtc aactactacc gccaaatgat
     4861 aggtgggata accatcagat tttaatagca cttgatcatc aactagatta gaattaaaac
     4921 taatttcacc acgtaataaa tcggtaaata caatatcctg attggcaggc actttgagtc
     4981 taattacata actttcacca gcggcaagtt ttttttgcac ctcttcctca cttaaatagc
     5041 gacagtgctg atcatagcgt ggtaattgat gattagcttg ttgctgttgc cgaacttctt
     5101 ctaaacgttg ttttgaacaa aaacaataat aagcctgacc tttagcaact agttcctgag
     5161 ctttttcttg ataaatgttg attctttgcg attgaatata tggaccatac gggccatctt
     5221 ttcctggacc ctcgtcccaa tctaagccaa atttgcgaat aatagcttga atttgctcaa
     5281 ctccgtttgg cacttcccga gttttatctg tatcttcaat tcttaaaata aactggccat
     5341 gatgccgttt cgcaaaggca tagtttttca acaaggtcgc catactagct acatgcatct
     5401 caccggtagg actgggtgcc attcttgtcc gaattggcaa ctgtttatct tgcataatta
     5461 ttctctctct aaataatttg gatgaatagc actcacatat gctaagaaat tatcatctcc
     5521 cttaaataca taaattggtt gtaaaaaggg ttgatattca aaatcatcaa aataagctag
     5581 ttcgacactc cgaatcaccg ctctttctaa attttgtcca ctagctatat aaccctctcc
     5641 agcctgaacc attttccaag cggatttaac tcctctcagt gggtaagtct caaaggttga
     5701 ataatccacc tcatgataat aataatcaac ttccacaatt gaattactac ctgtaaatgc
     5761 accgctaata accgctgaga tgactccctt ttctccagct ggagtgtaaa tggggtaata
     5821 atcatcaata ggcacccgat tcagatcaac agtgacaaaa tcagcatcag ataaagatag
     5881 ggccggagtt aattctccac ccaacgattt tttgtaacta attacacccg aactcgtagc
     5941 aacatcttta ggcatcagcc cgccctcgat caagaaattt ttaacgcgtt caactgcttc
     6001 aaattcctca ggcaagcgct tagtattatc ggtgattagc aattctggtt tagctaaata
     6061 attactgcta atggaaaaat ttaaataagt agcattaatt tccagactag tatctaaagg
     6121 ttgagtttta gtaaaacgat
//
LOCUS       DAOSEW010000045         5762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_12304_length_5762_cov_4.226213, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000045 DAOSEW010000000
VERSION     DAOSEW010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 5762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5762
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12304_length_5762_cov_4.226213"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..1444)
                     /locus_tag="PLJ02_02250"
     CDS             complement(170..1444)
                     /locus_tag="PLJ02_02250"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HOI05271.1"
                     /translation="MSKIIALIDCNNFYVSCERVFNPNLNHQPVIVLSNNDGCCVSRS
                     NEAKKIGIPMGAPYFELKDLIQRYNVKVFSSNYELYGDMSKRVLEVIKTFTDNVEFYS
                     IDEVFIGFDTAIHPPIELAEEIRSKILKWTGIPTSIGMAKTKTLAKAASEYAKKKTQS
                     NIFSLLEQPDIIFDAVLNWLEVGEVWGVGRQLTKKLQKIGINNAYQLAQLNEQTAKKI
                     GSVTLMRTVAELNQIKCLDLEKVTKMKKMIAATRSFAERVYSVNELEAAIVNHIVRAC
                     QKLREEQAVAKKMSIFVRTSYFGKIKYYSANQEITLETASNYPADFITRAIPLVQKIF
                     KKNTPFYKAGVCMQDIYPQDNLQQPLYQNNYIASFDKKQKIIRAVDLLNHKFGDNTVS
                     FSRHFPKNIWQMKHNLLSNHYTTKYSEFLQVY"
     gene            complement(1437..1820)
                     /gene="umuD"
                     /locus_tag="PLJ02_02255"
     CDS             complement(1437..1820)
                     /gene="umuD"
                     /locus_tag="PLJ02_02255"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013943661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HOI05272.1"
                     /translation="MQLALNWIQAGFPSPADDYTDSSFDIAKFLINHPAATFFVKVQG
                     DSMRDAGIFSGDLLVVDRAKKVKNGNVVVAFVDGEFTVKRFLKKLDQIILHPENEKFN
                     DIDVSQYSDFQIWGVVSFVIHNPNV"
     gene            complement(1865..2215)
                     /locus_tag="PLJ02_02260"
     CDS             complement(1865..2215)
                     /locus_tag="PLJ02_02260"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HOI05273.1"
                     /translation="MKKPNKKNQHYHDTPFGPYFKILDRIDKGKRYSKEFGSIMILSL
                     LEELGEMSRAYLAKHGRKKTNIAAQTDESYKQELGDLLVTILRFARIKNINLHEQILY
                     TLNKIEKRKIKPKQ"
     gene            complement(2239..2865)
                     /locus_tag="PLJ02_02265"
     CDS             complement(2239..2865)
                     /locus_tag="PLJ02_02265"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOI05274.1"
                     /translation="MPTSFSKQREAMLVNQLQARDITDQRVLEAFRQVPREKFVDPQL
                     RYRAYIDKPLPIKQEQSISQPYIVAKMCQLAQLTGTETVLDIGTGSGYQAAILSLLVK
                     QVFTIEIIEDLATEAKIKLKKLGYTNVEVIYGDGQQGLEKHAPYDLIKSAAATKEIPQ
                     AWKDQLQTGGKIILPLITQRGQELVKITKEKSSFKQENFGLVAFVPLI"
     gene            complement(2890..4491)
                     /locus_tag="PLJ02_02270"
     CDS             complement(2890..4491)
                     /locus_tag="PLJ02_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOI05275.1"
                     /translation="MINKELINDQPSEKAISPEVLDQRLLDYLQPSPKTEQQISLNLI
                     NTRLWLNELSQQYQRPITLDTIPDEAWADKLINYDIFWFMGIYSPSQFSANHAKKYTN
                     QYTYALPDIDAEKDVVASPFAVANYLPNPQLVKDWPTWDRMVEKLHQRDKKVFIDFVP
                     NHTAVDHPWANEHPEYFIQGSEGQYRAHPNFYQAINNDHGQVHYLAHGKDPNYPEWAD
                     TLQLNYGRPEVHQAMTEQLLSLVDHVDGLRCDMAMLLNPSTFLRTWAWALSNEERDYV
                     AKHSFWHEAIPQAKAKAMALGKSFDFMAEAYWEKNLLAENFDYIYRDELYKHLLRVAR
                     GENCHNLKEHIAYLLYCANDQQTCKDTVYLENHDEERAVRTMGREFSQAAAALISFLP
                     HTMLLINQGQEEGSTIKPPMQVGRFPQENKDLQLKNYYQNLLQLKQSKLFREGQWSMA
                     QLAHQEDNLITLEVVSQDRAIKALICINMGRNSSHFRIPQQASYQTLSCYDLNNDRYS
                     HLDAQNGNLELHLPAFNTQIVFFAS"
     gene            4655..4885
                     /locus_tag="PLJ02_02275"
     CDS             4655..4885
                     /locus_tag="PLJ02_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05276.1"
                     /translation="MSKESSNNFNYPVGAESAIAGAEFAIYNKSVDVFLKMLTDLKKQ
                     NPNIALEDLFALLSEALNEANEIVLLREPKSN"
     gene            4970..5722
                     /locus_tag="PLJ02_02280"
     CDS             4970..5722
                     /locus_tag="PLJ02_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05277.1"
                     /translation="MTNINPVRPQDNFNNPVAPSSGYNSPAATNLSSPLPSSSQSPTF
                     TGQTPEVAEQISSNKHKSVGDGANKLWLILLFCLAIILGFWAGYFTHQYFYQSNPVPN
                     TVINQVNQPTPVEQTFEPVSEVATAPAQINKEYIIFRDQEVYLNDGLDFTSQEQCVED
                     EIVILTAIEDDYLTIQMNEWTLQEDSGEYVASPIDFQVSDQACIAVRPICPNVSINRC
                     FSLELIDGVYHLDYEFIEEVAATTSSALEAEL"
BASE COUNT         1720 a         1009 c         1037 g         1996 t
ORIGIN      
        1 acttggttac tatccagcaa aaattgttat cccttttttt gatgaaaaaa aggagaaaaa
       61 aataatttat aaattttttt tcttacccaa tcttgacaaa aataaattaa aagatttttt
      121 tcaagaattt gcggattagc ttttatttaa aattatttga cttttatttt taataaacct
      181 gcagaaattc actatacttt gtcgtgtaat gattggataa aagattgtgc ttcatttgcc
      241 agatattttt tgggaagtgt cggctgaaac tgacggtgtt gtcaccaaat ttatggttga
      301 gcaaatctac agcccgaatt atcttttgct ttttgtcaaa acttgctatg tagttatttt
      361 gatataaagg ttgttgtaaa ttgtcttgtg gataaatatc ttgcatacat acccccgctt
      421 tataaaatgg ggtatttttt ttaaagattt tttgcactag cggaatggca cgagtaataa
      481 aatcagcggg gtagttggat gcggtttcca aagtaatttc ttggttagca ctgtaatatt
      541 taattttgcc aaaataacta gtcctaacaa agatactcat ctttttggca actgcttgtt
      601 cttctctcaa tttctgacaa gcccgcacaa tgtgattaac aatggccgcc tctaattcgt
      661 tgacggaata aacacgttca gcaaaagaac gagtggccgc aatcattttc ttcatctttg
      721 ttactttttc taaatctaaa catttaattt gatttagttc ggcgacggtg cgcatcaatg
      781 ttactgaacc aattttttta gcggtttgtt catttagttg agctaattga taagcattat
      841 taatcccaat tttttgtaac ttttttgtca gttgtcgtcc cacgccccac acttctccta
      901 cttccaacca attaagcacc gcatcaaaaa tgatgtcggg ttgttccaaa agggagaaga
      961 tattgctttg ggtttttttc ttagcatatt cactcgctgc tttagccaaa gttttagttt
     1021 ttgccatacc aatactagtg ggtataccag tccattttaa tatttttgat ctaatttctt
     1081 cagcaagctc tattgggggg tggatagccg tatcaaaccc aataaacact tcatcgatgg
     1141 agtagaactc aacgttatcg gtaaaagttt taatgacttc taaaacgcgt tttgacatat
     1201 cgccatataa ctcataatta gaagaaaaca ctttgacatt gtatcgttga attaaatctt
     1261 ttaactcaaa gtacggagcg cccatcggaa tgccgatttt tttagcttcg ttggaacgag
     1321 atacacagca accatcattg ttagataaaa caattactgg ttgatgattt aaattggggt
     1381 tgaatacccg ttcgcaggaa acataaaagt tattgcagtc gattaaggca atgattttag
     1441 acatttggat tatgaataac aaaactgacg acgccccaaa tttggaagtc actatattgg
     1501 ctaacatcta tatcgttaaa tttttcgttt tccgggtgca aaataatttg gtctaatttc
     1561 tttaaaaatc tttttacggt aaattctcca tccacaaaag ccactaccac gttaccgttt
     1621 ttaacttttt tagctcgatc aactactaac aaatctccag aaaaaattcc agcatcacgc
     1681 attgagtctc cctgaacttt aacaaaaaaa gtagcggctg gatgattaat taaaaactta
     1741 gcaatatcaa aactgctgtc agtataatca tctgccggag aaggaaagcc agcctgaatc
     1801 caattgagtg ctaattgcat caaagaatct taatacaaat aaaacccaaa aagcaagcgg
     1861 cagtttactg ctttggtttg atttttcttt tttcaatttt gttaagagtg tacaagattt
     1921 gttcgtgaag attaatattt ttgatacgag caaaccgcaa aatggtgacc aacaaatctc
     1981 ctagttcttg tttataagat tcgtctgttt gggcggcgat gttcgttttt ttgcgtccat
     2041 gtttggccaa ataagcacgt gacatttcac ctagttcttc aagcaagctc aaaatcataa
     2101 tggagccaaa ttctttggaa tagcgtttgc ctttatcaat tctatccaga attttaaaat
     2161 atggcccaaa tggggtatcg tgataatgtt ggtttttttt gtttggtttt ttcatcatat
     2221 ttctttccaa attattggtt aaattaatgg gacaaaagct accaagccaa aattttcttg
     2281 tttaaagcta gatttttctt tagtaatttt taccagttct tgaccccttt gagtaatcaa
     2341 aggtaggatt attttaccac ccgtttgaag ctgatctttc caagcttgag gtatttcttt
     2401 tgtggctgct gctgatttaa ttaaatcata gggagcgtgt ttttcaagac cttgttgacc
     2461 gtcaccataa attacttcta catttgtgta gcccaatttt ttaagtttta tttttgcttc
     2521 tgtggccaag tcttcaataa tttctatggt gaatacttgt ttaactaaca aactcaggat
     2581 ggctgcttgg tacccagagc ctgtgccaat atctaatact gtttcagtac cagttaactg
     2641 agctaattga cacattttgg ccacgatata tggttgagaa atactttgct cttgtttaat
     2701 cggcagaggc ttatcaatat aagctcgata acgcaattgc ggatcaacaa atttttctcg
     2761 aggaacttgt ctaaaagctt caagcactcg ttgatcagta atgtccctag cttgtagttg
     2821 attaacaagc attgcttctc tttgtttact gaagctggtt ggcataagat aacaatcaat
     2881 tacatcttat cacgaagcaa aaaaaactat ttgggtgtta aacgcgggca aatgaagttc
     2941 tagattacca ttttgggcgt ctaagtgcga atagcgatcg ttgtttaaat cataacaact
     3001 aagtgtttga taacttgctt gttggggaat tctgaagtga ctagagtttc tgcccatatt
     3061 gatgcaaatt agggctttaa tcgctcgatc ttgggatact acttctagtg ttatcaaatt
     3121 atcttcttga tgtgcaagtt gagccattga ccattgcccc tcacgaaaca attttgattg
     3181 tttaagttgt agtagatttt ggtaataatt ttttagttga agatctttat tctcttgcgg
     3241 gaagcgacca acttgcatcg gtggtttgat ggtgctgcct tcttcttgac cctgattgat
     3301 aagaagcatg gtgtggggca agaagctgat tagtgctgct gctgcttgag aaaattctct
     3361 tcccatggtt cttacagctc gttcttcatc gtgattttct aaatagaccg tatctttgca
     3421 cgtttgttga tcattggcgc aatataacaa ataagcaatg tgctctttta agttgtgaca
     3481 attttcccct cgggccactc tgagtaagtg cttataaagt tcatcacgat agatatagtc
     3541 aaaattttca gctaaaagat ttttttccca ataagcttcg gccataaaat caaaagattt
     3601 tcccagggcc atggctttag ctttagcttg aggaatggct tcgtgccaga aggaatgttt
     3661 agcaacataa tcgcgctctt cattacttaa agcccaagcc caagtacgca aaaaagtaga
     3721 gggattaagt aacatcgcca tatcacatct cagtccgtca acatgatcaa ccaatgataa
     3781 taattgttcg gtcatggctt gatgaacttc tggacgacca tagtttagtt gtaaagtatc
     3841 agcccattct gggtaattgg gatctttacc atgggctaag taatgaactt gaccatgatc
     3901 attattaatg gcttgataaa aattaggatg agctcgatat tgcccctcag aaccttgaat
     3961 gaaatattct gggtgctcat ttgcccaggg atggtcgaca gctgtatgat tggggacaaa
     4021 atcaataaaa acttttttat ctctctgatg aagtttttca accattctgt cccaagttgg
     4081 ccaatctttt actaactggg gattgggcaa ataattggca acggcaaagg gactggcaac
     4141 tacatctttt tcagcatcaa tatctggaag agcgtacgtg tattgattgg tatatttttt
     4201 agcatgattg gcactaaatt gactgggtga ataaatgccc ataaaccaaa aaatatcata
     4261 attaattagt ttatcagccc aagcttcgtc agggatggta tcgagagtga ttggtcgttg
     4321 atactgttga cttaattcat ttaaccacag tctggtgtta attaaattaa ggctaatctg
     4381 ttgttcagtt ttcgggctag gctgtaaata atctagtaat cgttggtcca aaacttcagg
     4441 agaaattgct ttttctgatg gttgatcgtt tatcagttct ttatttatca ttaacaaatt
     4501 tattaaaaca attttaaaaa ctttttcaat ttagcattct tctaggtagt tgtaaacctt
     4561 tgatgccttt tgatttaata tcaaataatt ttttaagtga tgatacaata aagaccttat
     4621 caaattaaaa ttttattcaa cggaagtcaa aacgatgtca aaagaatctt ctaacaattt
     4681 caattatcca gtaggagcag agtctgcaat cgctggggca gagtttgcaa tttataataa
     4741 aagtgttgac gtttttttaa aaatgttaac tgatttaaaa aaacaaaatc caaatattgc
     4801 cttagaagat ttatttgctt tgttatcaga agctctaaat gaagccaacg agattgtgtt
     4861 gctgagagag ccaaaaagca attaaaactt gtttacttcc attaattctt tcagattcta
     4921 attaaaaaat agcttgtttt ttattcttgg cttttgttat actaaagtca tgaccaatat
     4981 caatcctgtc cgccctcaag ataattttaa taatccagtt gcaccatctt ctggctataa
     5041 ttcgccagcg gctactaatt taagttctcc actaccttct agttctcaat cacccacttt
     5101 tactggtcaa actccagaag ttgctgaaca aatttcaagt aacaaacaca aatcagttgg
     5161 tgatggggct aacaagttgt ggctaatttt gttattttgt ctggcaataa ttcttggttt
     5221 ttgggcaggt tattttactc atcagtattt ttatcaaagc aatccggttc caaacacggt
     5281 aattaatcaa gttaatcaac cgactcctgt tgagcaaact tttgaaccgg tgtctgaagt
     5341 agccacagcg ccggcacaaa ttaataaaga atatattatt tttagagatc aagaggttta
     5401 cttaaacgac ggcttagatt ttacgtccca ggagcaatgt gttgaagatg aaattgttat
     5461 tttaactgct attgaagatg attatttaac tatacaaatg aatgaatgga ctttgcaaga
     5521 agattctggc gaatatgtag ccagccccat tgattttcaa gttagcgatc aggcatgtat
     5581 tgcggtaaga ccaatttgtc ccaatgtttc tattaaccgt tgcttttctc tagagctaat
     5641 tgacggagtt tatcacttag attatgagtt tattgaagaa gttgctgcca ctacttcttc
     5701 agcattagaa gccgagcttt aaattacttt ttttaagttt tgtcaaaaaa tagctgatgt
     5761 tg
//
LOCUS       DAOSEW010000046         5726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_12403_length_5726_cov_4.282314, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000046 DAOSEW010000000
VERSION     DAOSEW010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 5726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5726
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12403_length_5726_cov_4.282314"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="PLJ02_02285"
     CDS             complement(<1..477)
                     /locus_tag="PLJ02_02285"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HOI05278.1"
                     /translation="MTKWVIHQHSQQLLKQLPVFMPDATLGVVRSLSPNDLQAIGLNW
                     LMVNTFHLLKQPGEKTLEQAGGLKNLMNWPGFLASDSGGFQLFSLIQKNPNLGKITDE
                     GVILYSGPKKQKKTLFTPEKSIRLQFAIGSDIKIVLDDFSPPDADEARLETSVKRTI"
     gene            531..749
                     /locus_tag="PLJ02_02290"
     CDS             531..749
                     /locus_tag="PLJ02_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOI05279.1"
                     /translation="MKGKMPTQKKTIKRLTRSKTDRKIAGVAGGLAHYFGIDSVLVRL
                     IFALACLPGGFPGLLAYIIAWIVMPLED"
     gene            complement(954..1757)
                     /locus_tag="PLJ02_02295"
     CDS             complement(954..1757)
                     /locus_tag="PLJ02_02295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOI05280.1"
                     /translation="MGTYHYPASKTERGIHMSPDDKNVSILDEREKRLMTTMLKQTIP
                     QKFFQPSIKILDVGAGTGKLIKTLSIEGKYTLVEPDPKRADLAKKRVCLTKDQVIVDF
                     FQNISLPSETFDLIFCIHLAQHVSEDDWEKIIKNISKIIKPGGYLVLAFTVKNSLFGT
                     YNLQWTNKNDRTMNKVSRSEFNHIAKKHNPNILPIKKIDIDEMINQLSNLSFKVLRYE
                     YYSPIFITRKQKTLQWFLKRLTSDLQNKILKIVRYRYYYDCIMIVRKKS"
     gene            2008..3153
                     /gene="ychF"
                     /locus_tag="PLJ02_02300"
     CDS             2008..3153
                     /gene="ychF"
                     /locus_tag="PLJ02_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009278095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOI05281.1"
                     /translation="MSLSLGIVGLPNVGKSTLFNALLKKQVALAADYPFATIQPNIGV
                     VEVPDERLEVLANITQQSEKLANLPPIKHALVEFVDIAGLVKGAAQGEGLGNQFLANI
                     RETQAICHVLRAFANAKVVREGSISPIEDLATVRTELQLADLATLNKQVAPKGTVSKT
                     EQWRWSIIEEFKKTLEQGRNILVSKANLNNHQAVTGKSALNKDEALVAKELCLLTAKP
                     EIFVINVDEKDLSQAEDLKGKWAKQLDVPIEQVVIISAQIESQLAVLDTTDQQAYLQD
                     LGLDESGLERLAKVAYDILQLQSFLTAGEIEVKAWTIEKGATARQAAGVIHSDFYDKF
                     ISARVISFEDFVKLGGWQSAKEAGKIRQEGRDYLMQDGDVVEFMVGR"
     gene            3274..3663
                     /gene="rpsF"
                     /locus_tag="PLJ02_02305"
     CDS             3274..3663
                     /gene="rpsF"
                     /locus_tag="PLJ02_02305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOI05282.1"
                     /translation="MKLVKNSSQRDYEVTYLLTGDLLDAEVAKIRKEITDLVTKLKGK
                     VVEEQDWGRKLLAYKIRHLGTAHQEAIFTHLIVQLAPEKVRKLNKELDLHRQIMRHLI
                     VQKEAGEAVEKKESETKEDIKEAESKK"
     gene            3742..4260
                     /locus_tag="PLJ02_02310"
     CDS             3742..4260
                     /locus_tag="PLJ02_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOI05283.1"
                     /translation="MSVRSLNKVMLIGNLTRDPNLRFTPNGTAVCSFGLATNRSWTSS
                     DSGEKQERVDFHNVVAWGKLAEICGQLLHKGDKIYVEGRIQTRDWKTEDGAERRITEI
                     VIDNMMLLSSRNGYSVSADEEEEGDKEVKTKGQKSKKELADEAIADEVVSEEIPEGEA
                     EDVEDVSDDIPF"
     gene            4303..4461
                     /gene="rpsR"
                     /locus_tag="PLJ02_02315"
                     /pseudo
     CDS             4303..4461
                     /gene="rpsR"
                     /locus_tag="PLJ02_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014038.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
     gene            4538..>5726
                     /locus_tag="PLJ02_02320"
     CDS             4538..>5726
                     /locus_tag="PLJ02_02320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOI05284.1"
                     /translation="MPINNEQNSENLTNFQEATMPKSKKSLFKPLARIVTSLLLITLG
                     VVIGYRYALGNLPFNVKLGFLDQIANYQRNEDLTQLMRSSKVQAPVSMDVFWEVWQIV
                     ERDYYDSSKIDYNKMMDGAISGMVWGLGDPYTAYLPPEENEQTGEDLAGSFYGVGIEL
                     GYVDGIVGIIAPVAGSPAEKAGVKAGDMILHVKDEAAGLDEDTSNWTLDKAVQNIRGP
                     KGAPVTLTLFQPGENDSREVEIVRDEIIVESVVLEYVEVAGKPFAHLKLSRFGDRTQA
                     EWDSAVISILNKWNNQEIVGLIFDLRNNPGGYFDTAINIASDFVRTGTVVSQKGKFSQ
                     QDYPSTGKARLEKVPTVLLVNKGSASASEIVAGALRDDVGIKLIGEQTFGKGTVQDRR
                     ELSN"
BASE COUNT         1816 a          882 c         1215 g         1813 t
ORIGIN      
        1 aatggttctt ttcacacttg tttccaggcg cgcctcatcc gcatctggtg ggctgaaatc
       61 atctaaaaca attttaatgt cactgccaat agcaaattgt aaacggatcg atttctctgg
      121 agtaaacagc gttttctttt gtttttttgg tccactgtac aaaattacac cttcatcggt
      181 aattttgcct aaattggggt ttttttgaat tagcgaaaat agctgaaaac ctcctgaatc
      241 agaagccaaa aagcctggcc aattcattaa attttttaag ccaccggctt gttctaaggt
      301 cttttcgcct ggctgtttca gcaaatgaaa ggtattcacc atcaaccaat ttaagccgat
      361 ggcttgaaga tcgttggggc taagactacg cacgacacct agagtagcat ctggcataaa
      421 aaccggcagt tgttttagta gttgttgcga atgttgatgt attacccatt ttgtcatcag
      481 gcatatttta cttgatcagt tataataaag ctaaagaaat tgaaaattac atgaaaggca
      541 aaatgccaac tcagaaaaaa actattaaac gtttaactcg ctccaagaca gacagaaaaa
      601 ttgctggcgt ggctggcggt ttagcgcatt atttcggcat tgattccgtt ttggtgcgtt
      661 taatttttgc tttagcttgt cttccaggtg gatttccagg attattggct tacattattg
      721 cttggatagt tatgccactt gaggattaat attctcaata aataattggc aaaagcccca
      781 tcctttcaga tggggctaat tgcagttgtt catttatttt tttgaatttt gatattccgc
      841 ttgcgcggtt gcttgaactt caatcagtgt gaatgcccga ttttttctat gtgtcatatt
      901 ctttttattt gactccaaac attcaactcc aaatattaaa aattgttttt aaattatgat
      961 ttttttctaa ctatcattat acaatcatag taatatctat atctgacaat ttttaggatt
     1021 ttgttttgta ggtcagatgt caatcgttta agaaaccatt gaagtgtttt ttgttttcta
     1081 gtgataaata ttggggaata atattcataa cgcaatactt taaatgacaa atttgataat
     1141 tgattaatca tttcatctat atcaattttt tttatcggta atatgttagg attgtgtttt
     1201 tttgcaatat gattaaattc agatcgtgaa actttattca ttgttctatc gtttttattt
     1261 gtccattgta agttatatgt tccgaacaat gagtttttta cagtaaatgc caaaactaag
     1321 tatccacccg gctttattat tttagagata ttttttataa ttttttccca atcgtcctca
     1381 gagacatgtt gggccaaatg tatacaaaat attaaatcaa aagtctcaga aggaagtgat
     1441 atattttgaa aaaaatctac aataacttga tctttggtta aacaaacacg ttttttagct
     1501 agatctgctc ttttggggtc tggttcaaca agtgtgtatt ttccttcgat tgataatgtt
     1561 ttaatcagtt ttccagttcc tgcaccaaca tctagtatct ttattgatgg ttgaaaaaat
     1621 ttttgaggga ttgtttgctt aagcatggtt gtcataagcc gtttttccct ttcatccaaa
     1681 attgagacat ttttatcgtc tggtgacata tgtataccac gttccgtttt tgaagcagga
     1741 taatgatatg ttcccatatt gtttacaatt atacactgag attatctatt gcatttaaac
     1801 ttaatcattt cttttgatat tatttaatgt gaaaagtaaa aattttttaa ttatgaaatg
     1861 aaacaaaatt tacattgaat atgtaaattg gtgtgtaaaa agaagtatta gagatacaat
     1921 agatgaagta attatttcaa aaaggatcgg taaaaaatta tggtggagga ttatccttgt
     1981 tgaccataag caccgagtat ttttaatatg agtttaagct tgggtattgt tggtttgccc
     2041 aatgttggta aatcaacttt atttaatgcg ctcttaaaaa aacaagtggc gctagctgct
     2101 gactatccct ttgctaccat tcagccgaat attggtgtag ttgaagtacc agacgagcgt
     2161 ctggaagtgt tagccaatat tacccagcaa agtgaaaaat tagccaattt accaccaatt
     2221 aaacatgctt tggtcgaatt tgtggacatt gccggtttag tgaagggtgc tgcacaaggt
     2281 gagggtttgg gcaatcagtt tttggccaat attcgtgaaa cccaggcaat ctgtcatgta
     2341 ttgcgagctt ttgccaatgc taaagtagtg cgggaaggaa gtatttcacc aattgaggat
     2401 ttagcaactg tccgcaccga attacagttg gctgatttag ctactttaaa taaacaggta
     2461 gcgcccaaag gcacagtttc caaaacagaa caatggcgct ggtcgattat agaagagttc
     2521 aaaaaaactc tggaacaagg acgaaatatt ttagtgagta aagccaatct taacaatcat
     2581 caagcagtaa ctggcaaatc ggcactcaat aaagatgaag ccttggtagc caaagaattg
     2641 tgtttgttaa ctgctaagcc agaaattttt gtgattaatg ttgatgaaaa agatttgagc
     2701 caagcagaag acttaaaagg caagtgggct aaacaacttg acgtaccgat tgagcaagtg
     2761 gtgattatct ctgctcaaat tgaaagtcag ttagcggttt tggataccac agatcaacaa
     2821 gcctatcttc aggatttggg tttagatgaa agtggtttag agcggttagc taaagtggcc
     2881 tatgacattc ttcaattaca aagttttttg actgctggtg aaattgaagt caaagcgtgg
     2941 acgatcgaaa agggggccac cgctcggcag gcagctggcg taattcatag tgatttttat
     3001 gataagttta ttagcgctag agtgattagt tttgaagatt ttgtcaaact cggtggttgg
     3061 caatcagcta aagaagctgg caaaattcgt caagagggta gagattatct gatgcaagat
     3121 ggggatgtgg tggagtttat ggtagggcgc taatttttgc ttgtaatttc aagcattcta
     3181 tactagaata ccctctgagt tcgctaaaat ttattaatta tccaaggaaa aattgttatc
     3241 cggcaatttt ttccgcgaac tagaatggaa cagatgaagt tggtgaaaaa ttctagccaa
     3301 agagattatg aagtaactta cctgttaaca ggtgatcttt tggacgctga agtagcaaaa
     3361 attcgcaaag aaattaccga tttggttacc aagctcaaag gcaaagttgt tgaagaacag
     3421 gattgggggc gcaaactatt ggcttacaaa attcgtcatt taggtacagc tcatcaagaa
     3481 gcaattttta ctcacctaat tgtgcaatta gctccagaaa aagtgcgtaa attaaataaa
     3541 gaactggatt tacatcgcca gattatgcgc cacttaattg tacaaaaaga agcgggtgag
     3601 gctgtagaaa aaaaagagag tgaaactaaa gaagatatta aagaagcaga aagtaaaaaa
     3661 taaaacataa cagattttgt catttaaaag atattaatat gataatgtta tgaaataact
     3721 ctgttacaga gaaaggaaaa aatgtcagtc agatctttaa acaaagtaat gttaattggc
     3781 aatttaacta gggatccaaa tttaagattt actcccaatg gaacggcagt ctgttctttt
     3841 ggtttagcaa ccaatcgttc ttggacgagt tctgatagtg gagaaaagca agaaagagtt
     3901 gattttcaca atgtcgtcgc ttggggtaaa ttagccgaaa tttgtggtca attactgcac
     3961 aaaggtgaca agatctacgt ggaaggcaga attcagaccc gtgattggaa aactgaagat
     4021 ggggcagagc gacgtatcac cgaaattgtt attgataaca tgatgctgtt aagcagccga
     4081 aatggctaca gtgttagcgc tgatgaggag gaagaagggg ataaagaggt taaaaccaaa
     4141 ggccaaaaaa gcaaaaagga attggctgat gaagcaatag ctgatgaagt ggtatcagag
     4201 gaaattcctg aaggtgaagc tgaagatgta gaagatgtca gtgatgacat tcctttttag
     4261 tattattaaa aaatgaaagt tttcatgaaa caacacttta gttataaaaa tccagatgcg
     4321 cttcgtaagt ttattacccc tttcggttca attttaccta gaagcaaaac tggtttaagt
     4381 gccaaagagc agaaaaaatt agccagagaa atcaaaagag ctcgtcattt agctctttta
     4441 ccgtttactc agactttata acattaatga tttttaataa aagaagctag tttttaacta
     4501 gctttttttt atgcttcgtt ttacaatgat gtgccagatg ccgattaata atgagcaaaa
     4561 ttccgagaat ttaactaatt ttcaagaagc aactatgcct aaatcaaaaa aatcattgtt
     4621 taaaccacta gcacgtattg tgaccagttt gttacttatc acgttgggag tagttattgg
     4681 ttatcgttat gcgttgggta atttaccttt taatgtgaag ctcggttttt tggatcaaat
     4741 cgccaactat caacgtaacg aagacttgac tcagctcatg cgaagttcaa aagtgcaagc
     4801 accagtctca atggatgttt tttgggaggt gtggcaaatc gtggaacgag actattatga
     4861 ttcaagtaag attgattaca ataagatgat ggatggggcc atttctggta tggtttgggg
     4921 tttaggtgat ccttatactg cttatttacc tcctgaagaa aatgaacaaa ctggtgaaga
     4981 tttagctggt agtttttatg gggtaggcat tgagcttggt tatgttgatg ggatagtggg
     5041 aattattgca cccgtagctg gtagcccggc agagaaagca ggagtgaagg ctggggacat
     5101 gattttacat gttaaagatg aagctgccgg tcttgatgag gatacaagca attggactct
     5161 agataaagcc gtacaaaata ttcgtggtcc aaaaggagcg ccagtgacat tgacactgtt
     5221 tcagccagga gaaaatgaca gtcgagaagt ggaaattgtg cgagacgaaa ttatcgtaga
     5281 aagtgtggta ttagaatatg tggaagttgc aggtaaacca tttgctcatc tcaaattaag
     5341 tcgttttggt gatcgcactc aggcggaatg ggacagtgct gtcatttcga ttttaaacaa
     5401 gtggaataat caggaaattg tcggattaat ttttgatctg cgcaataacc ccggtggtta
     5461 ctttgataca gctattaata tcgctagtga ttttgttcga acaggcacag tggtgtcgca
     5521 aaaaggcaaa tttagtcagc aagattatcc cagcactgga aaagcgcggt tagaaaaagt
     5581 accgactgtg ttgttagtta ataaagggag tgcaagtgct agcgaaattg ttgctggtgc
     5641 cttgcgtgat gatgttggta ttaaactaat tggtgaacaa acttttggta aaggtactgt
     5701 ccaagatcga cgcgagttaa gtaacg
//
LOCUS       DAOSEW010000047         5712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_12449_length_5712_cov_4.388722, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000047 DAOSEW010000000
VERSION     DAOSEW010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5712
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12449_length_5712_cov_4.388722"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..1042)
                     /locus_tag="PLJ02_02325"
     CDS             complement(62..1042)
                     /locus_tag="PLJ02_02325"
                     /inference="COORDINATES: protein motif:HMM:NF024078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HOI05285.1"
                     /translation="MKQHPIPQDITGYKFHLIGAMTLKQFAELAVGAIIAFIVYQSNL
                     IGIVKWPMFIMLIVLAIMIAFVPIEERPLDFWIFTYFKNLWKPTKFFWKKTPKIPEFF
                     NYKPGQHQDLDIKPDVNYSPARKERIQEYLKSIPDSDEELDEWDISYNQRANELLADF
                     DQVQIKAENISVQREPKTGKPQLKTRIRKLKSLEDIQEQQTPKIELTSTNKEPDKLSP
                     STTVKTEANQSHNLEQPSIKEEKSNQLQGIIKDSRGQAVGNALIEVFAKNEDQALRIT
                     KSNTNGEFFIKTALPNGEYQIKTEADGLVFMPISIRLTGRTVPTIEIVAE"
     gene            complement(1039..1449)
                     /locus_tag="PLJ02_02330"
     CDS             complement(1039..1449)
                     /locus_tag="PLJ02_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05286.1"
                     /translation="MKNKKKRTIIFISILIVILLILLLARERTPKDTMPSPPPTNQPE
                     EPVLRDAFPIDEPVLSEEEEIELQQVTELKNMSPITTPEFSIEYSYKTGGFIVKSNLG
                     LDATEKALKIWLNKQGFNAINSERFEYQSNKLSL"
     gene            complement(1451..4870)
                     /locus_tag="PLJ02_02335"
     CDS             complement(1451..4870)
                     /locus_tag="PLJ02_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05287.1"
                     /translation="MTDQGNVDNFRLELKRLASQNSLSLDEIQAIIEFCGNFFSQDLP
                     QKFGKSFNCPFLIQGFSGQTFKLARVNKIESQEKFIADLLKDKEIAQSQKDRIIAELK
                     DQQKKILEENLFLSDLIAFLEQSNILLAKFLDPSHKQELAKLGNDFFSRIGLAAKTSS
                     TQVLLKELKKLEESPSIWQKVNSDLAQPMLTIISSQKEKFSAQSKDEEEKKEDLVDSA
                     AVQDKKKAEPSSASKVEEEGQDSDSEKEEEKEKPKQTLPEDFSKEFNLARLDDLSKLY
                     IRSLAIISINQSLQIQFANLSKEQLSKMGFDHPPNFNQLPVASRQELLTITFSKIETL
                     LASGRYDLDQLINTPSLRISFSKEIALKIIADIRGGEIFARQLGELTSSELDAQAVQK
                     ELKKNEELHLDEEQLLKVKINNASLPAELKIHLQNLSTEEANNFFAQLDTNSALKNQF
                     SKNLKLTFTRVTKNNFSQEWQRIIVGDKEAQKRSFLARENITPIIDVFIQQNYPVDYV
                     ESFNWQRFQKHFGKDVIDQNTYMANERAIKDLLISYWKTQRADWSRTIHQGIDNEFYT
                     TADLETLWQEFDQIKADGSGSKGAQTKENKKLQQKQTVYFQQIQHQAHLNLVNADAVS
                     KRLAGFAQDNIDFAGFSPQQINELNYLNYVYQQSFLDVINGGGPELQTIALTYYVPGE
                     DYTLDANTVLQTDSLPQFSAYDLGALNYNQYFLPNEDGAYPLARSFNEEGGIDNQLGE
                     SFGAVKDKSKQAINVLGEKAVRVGLNYATGGAFESLPEPVKQMIEQMAWGAVKQKLEP
                     LIKALIALISAAFAALLALIFQVVKIVSQAISFLGFGGGGKEVTPVIAQNTFWQNVEN
                     TINPATLSPQNLPIAENQLAERVTAQTQAAQQARTAADVAVRPVSAQIGSSLSQIGQG
                     AMATATQAVLTTFGLAAGGMLLYQTILDGAFLTQFPTGGSGGGGGAVFCNDVVVGNLT
                     YYSQREYNDIPICGGGCSFGSSACGPVSISMILNEDPIDVSMREGFLVPGGCGLTSCS
                     GTAIAPLINALSNNGVSAVSVPTPSGSPEQITDEISRYLAEGNVIFALTHTRGFGHFY
                     VITCVESPGYITAYDPWWGQNVVHKVVSTSGEGLASGTDNTYIRYMYLVQN"
     gene            4940..>5712
                     /locus_tag="PLJ02_02340"
     CDS             4940..>5712
                     /locus_tag="PLJ02_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05288.1"
                     /translation="MDMNINSGFSPEKGFFRDADGGGAAGGVGEGNNNDQQPSPEEES
                     EKSKENHENGENHENGEGQSAGASVGDSETVDNEKKDREPLNIFDQLRANLKEIATLI
                     SDNRPKAATDLLRGSRELIKNYQPDSEDEKNALLFFLDRLKLKAKALYSDPDIDSLDE
                     LAKKIKEQKKNSAGPTESAAQTGQDEGTGETGLVRQTEQTDQTKQAEQAEAIKTVEVG
                     EYGKIPPWQGKFDRLWNILQDTYRKYPSSSPEIYDLVSQL"
BASE COUNT         1642 a         1046 c         1142 g         1882 t
ORIGIN      
        1 ctgtatctaa gtttactaga ttttgtctgt taataaaaga ccgttaataa gagtcatgac
       61 cttattcagc cacaatttca attgtaggaa ccgtacgacc cgtcaatcta atggagattg
      121 gcataaaaac taagccatcg gcctcagttt taatttggta ttctccgtta ggaagagcag
      181 tcttgataaa aaactcgcca ttagtgttgc ttttagtaat tcttaatgcc tgatcttcat
      241 tttttgcaaa cacctcaatc aaagcgttac caacagcttg acccctgcta tcttttatta
      301 tgccttgtaa ttgattgctt ttttcttctt ttattgatgg ttgctctagg ttgtgagatt
      361 gattagcttc cgttttgaca gttgtggatg ggcttaactt gtctggttct ttgtttgttg
      421 atgtcaactc aatttttggt gtttgttgtt cttgaatgtc ttctaaactt ttaagttttc
      481 taattctggt tttaagttgg ggtttgccag tttttggttc acgttgaacc gagatgtttt
      541 cagctttaat ttgtacttga tcaaaatcgg ctagcagttc attggcgcgc tgattataac
      601 taatgtccca ttcatcaagc tcttcatcgc tatccggtat gctttttaga tattcttgaa
      661 tgcgctcttt tctggcggga gaatagttta catctggttt aatatcaaga tcttgatgtt
      721 gtcctggctt gtagttaaag aactctggaa tttttggagt ttttttccaa aaaaatttag
      781 tcggtttcca aagattttta aaataggtaa agatccaaaa atctagtggg cgttcttcaa
      841 ttggcacgaa agcgatcata attgcgagga caattaacat aataaacatt ggccacttga
      901 cgataccaat caaattgctt tgataaacaa tgaaggcaat tattgctcca accgccaatt
      961 cagcgaattg tttgagggtc atggccccga ttagatgaaa tttatagcca gtaatatctt
     1021 gtggaatggg gtgttgtttc ataaggacag cttatttgat tgatattcaa agcgctcact
     1081 gttaatcgca ttgaatcctt gtttatttaa ccatattttt agcgcctttt ctgtggcgtc
     1141 taaacccaaa tttgatttga cgataaaacc cccagtttta taagagtatt caatactaaa
     1201 ttctggtgta gtaattggtg acatgttttt gagttctgtt acttgttgca attcaatttc
     1261 ttcttcctca ctcaacacgg gttcgtctat ggggaaagca tctcttaata ctggttcctc
     1321 tggttgattg gttggtgggg ggctaggcat ggtatccttt ggtgttcttt ctctagcgag
     1381 taaaagaatt aataaaatta caattagtat gctgataaaa atgatggttc tctttttttt
     1441 gtttttcatt ttaattttgc actaaataca tatatctaat atacgtgttg tcagttccag
     1501 atgccaaccc ttcgcccgat gttgatacta ccttgtgcac cacgttttgt ccccaccacg
     1561 gatcgtaggc ggtgatgtat cctggagatt caacgcaggt aatcacgtaa aagtggccaa
     1621 aacccctggt atgggttaag gcaaaaatta cgttaccctc ggctaaatac ctactaattt
     1681 catcggtgat ttgttcgggg ctaccagagg gtgttggtac tgaaacagca ctaacaccat
     1741 tattactgag agcattaatt aatggagcta tggcagtacc actgcaactt gtcagaccac
     1801 agcctcctgg tacaagaaat ccctcacgca tagaaacgtc gattggatct tcatttagaa
     1861 tcatggaaat tgatactgga ccacaagcag aagaaccgaa actgcagccc ccaccacaaa
     1921 tcggaatatc gttgtattct ctttgactgt aataagttag gttgccaact accacatcat
     1981 tgcaaaaaac cgcaccgcca ccacctccgc ttccgccagt agggaactgg gttaaaaaag
     2041 cgccatccaa aatagtttga taaagtaaca tgcctccggc tgccagacca aatgtagtta
     2101 aaacagcctg agtggcagta gccatagcac cctggcctat ttgtgaaaga ctggaaccaa
     2161 tttgagctga cactggacga actgcaacgt cggctgcggt tcgggcttgt tgggcggctt
     2221 gagtttgagc agtcactctt tcagccagtt gattctcggc aatgggtaga ttttgcgggg
     2281 ataaagtagc gggatttatg gtgttctcaa cgttctgcca aaaagtgttt tgggcgataa
     2341 ctggtgtgac ttcttttcct ccaccaccaa accccaaaaa agaaatagct tgagaaacaa
     2401 ttttaaccac ctggaagatt agggccagca atgccgcaaa agcagctgaa attaaggcga
     2461 taagagcttt gattaatggt tctaattttt gtttaacagc tccccaggcc atttgttcaa
     2521 tcatttgctt cactggttcg ggtaaacttt caaaagcacc accagtggca taattcaagc
     2581 caactctaac cgccttttct cccaaaacat ttattgcttg cttgctttta tctttaacgg
     2641 caccaaaact ttctccgagt tgattatcaa tccctccttc ctcgttaaaa gatctagcga
     2701 ggggatatgc tccatcttca ttggggagaa aatattgatt gtaattaagt gcccccaaat
     2761 cataagcgga aaattgtggc aaagaatctg tttgtaagac tgtgttagca tccaaggtat
     2821 aatcttctcc cggcacgtag taagttaaag ctatagtttg cagttccggg ccaccaccgt
     2881 taattacatc aagaaaactt tgttgataaa cataatttag ataattaagt tcgttaatct
     2941 gttgtggact aaatccagca aaatcaatat tatcttgagc aaatccggct aatcttttac
     3001 ttactgcatc ggcattgact aggttaagat gggcttgatg ttgaatttgt tgaaaataaa
     3061 ctgtttgttt ttgttgcagt ttcttatttt cttttgtttg agctcctttg cttccagacc
     3121 catctgcttt gatttgatca aattcttgcc ataaagtttc taaatccgct gtggtgtaaa
     3181 attcattatc aataccctga tgaatggtac gactccaatc ggctcgttgg gttttccaat
     3241 agctaatcag caaatcttta atggcccttt cattggccat gtaggtgttt tgatctataa
     3301 catctttgcc aaaatgtttt tggaaacgtt gccaattaaa gctctcgaca tagtcaaccg
     3361 gatagttttg ttgaataaaa acatcaatga tgggtgtaat attttctcgc gctagaaatg
     3421 atcttttttg tgcttcttta tcaccaacaa tgattctttg ccattcttga gaaaaattat
     3481 tttttgtgac tctagtaaaa gtcagtttta aatttttaga aaattgattc ttaagggcgc
     3541 tattcgtatc gagttgagca aaaaaattgt tcgcttcttc ggtggataag ttttgtaaat
     3601 gaattttgag ttcggcaggt agagaagcat tattaatttt tacttttaat aattgttctt
     3661 catctagatg aagctcttcg ttttttttaa gctctttttg aactgcctgc gcgtccaatt
     3721 cgctgctggt taattcgcct aattgacgag caaaaatctc tccaccacga atatcggcaa
     3781 tgattttcag ggcaatttct ttggagaaac taattcttaa tgagggagta ttaattaatt
     3841 gatctaagtc ataacggcca ctggccaata aggtttcaat tttgctaaag gtaatggtta
     3901 ataattcttg tctgcttgca actggtagtt ggttgaagtt gggtgggtga tcaaagccca
     3961 ttttagacag ctgctctttg gataaattag caaactggat ttgtagagat tggttaatcg
     4021 aaataatggc tagtgagcga atgtatagtt tggagagatc gtcgagacga gccaaattaa
     4081 attctttaga aaaatcttca ggcagggttt gttttggctt ttctttttct tcttcttttt
     4141 cgctatctga atcctgaccc tcttcttcaa ctttgctggc agaacttggt tcagctttct
     4201 tcttgtcttg aacagctgct gagtctacta aatcttcttt cttttcttct tcgtctttac
     4261 tctgagctga aaacttttct ttttgtgagg aaataatggt caacattggt tgagccaaat
     4321 cactatttac cttttgccaa atagaggggc tttcttccaa ttttttaagt tctttaagta
     4381 aaacttgagt tgaactcgtt tttgcggcta aaccaatgcg actgaaaaaa tcattaccaa
     4441 gtttagccaa ttcttgttta tgacttggat cgaggaactt agccagcaaa atattacttt
     4501 gttctaagaa ggcaatcaga tcacttaaga atagattttc ttcaagaatc ttcttttgtt
     4561 gatcttttaa ttccgcaatg attctgtctt tttgactttg agcgatttct ttatctttta
     4621 atagatcagc aatgaatttt tcttggctct caattttatt aaccctggca agcttaaagg
     4681 tttggccact aaagccttga attaaaaaag gacaattaaa acttttaccg aatttttgcg
     4741 gtagatcttg gctaaaaaaa ttgccacaaa actcaataat tgcttgtatt tcatcgagac
     4801 taagactatt ttggcttgca agccttttga gttctaggcg aaaattatcc acattgcctt
     4861 gatcagtcat ggttttatta tagacagaaa tttaaggagt gactaggcaa aaaaaatcaa
     4921 agatagtata atttgcacta tggatatgaa tattaacagt ggtttttcac cagaaaaggg
     4981 attttttaga gatgccgatg gaggcggagc cgcgggggga gtgggtgaag gcaacaacaa
     5041 tgatcaacag ccatctcccg aagaagagag tgaaaaaagt aaagagaatc atgagaatgg
     5101 agagaaccat gagaatggag aaggacagtc agcaggcgct tccgtaggtg atagtgaaac
     5161 tgttgataac gaaaaaaaag atcgggagcc tttgaatatt tttgaccagt tgagagctaa
     5221 tttaaaagag attgccacac taattagcga caatagacct aaagctgcca ctgatttatt
     5281 aaggggatcc cgagagttaa taaaaaatta tcagcctgat tctgaagatg aaaagaatgc
     5341 tttgcttttt tttctggata gattgaaatt aaaagcaaaa gctttgtatt ctgatcctga
     5401 tatagatagt ttggatgagc ttgcaaaaaa aattaaagag caaaaaaaga attctgctgg
     5461 gccaactgaa tcggctgcac aaacggggca agatgaagga actggagaaa ctgggctggt
     5521 caggcaaaca gaacagactg accagaccaa gcaggctgaa caagccgaag caataaaaac
     5581 tgtggaagtt ggtgaatatg ggaaaatacc tccttggcaa ggaaaatttg atcgactatg
     5641 gaatattttg caggatactt atagaaagta tcctagctct tctccagaaa tctatgattt
     5701 agtttcccaa ct
//
LOCUS       DAOSEW010000048         5540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_13009_length_5540_cov_4.725615, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000048 DAOSEW010000000
VERSION     DAOSEW010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 5540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5540
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13009_length_5540_cov_4.725615"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /gene="rplJ"
                     /locus_tag="PLJ02_02345"
     CDS             complement(<1..423)
                     /gene="rplJ"
                     /locus_tag="PLJ02_02345"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOI05289.1"
                     /translation="MPSQDNIEQVKIIQDKIDRAKSLVIVDYAGTSVNDLTTLRNALR
                     KAGGEMFVTKNTLIDIALGKGKVSESLTGMNAIVFSYEDAIAGLKVLFDFHKNQDKLS
                     IKQGFMEDKVLSVAEVEALSKLPSKSELVVMLIQRLKNP"
     misc_feature    complement(446..573)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            638..2839
                     /locus_tag="PLJ02_02350"
     CDS             638..2839
                     /locus_tag="PLJ02_02350"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOI05290.1"
                     /translation="MSKQIVMVKKVLILVILLCFLTSSLSFSQPVSANSYAIDRATIE
                     IFLQNDGSAKVIEQRVFNFQGDYRFVYHKIFYQNDQTLSAVGRSEPYLLNNFALCEVD
                     QSSGADQYNCYELLEQNQVNLTEPRHNPDNTFYVEQRAKEYFVQWHYQAINEKKIFEL
                     RYEVENLLTQQGDVAELYWQFVGDAWEVAHGAIDITVWLPTNYLASQLRDVQAWGHGP
                     LAGTVRIDALPGDNGLYGVHFEAPALPMNQFLEGRVIFPLEALLETQALPIGKLNREK
                     IIEQEQAYIAETAAKLKREETIAKVFFAGALLLSILELVWFVQSLIKHLKLAKEERLT
                     ALPGVWEPPSDLEPALVYQLLYKTKKLNPAVFTATVLSLVHKKVCRIVRSEQKEGFIV
                     KDYRYFLEPTDKVGQVKLSEVEEKVFEFLFKVVGGSYIRYYHQASGKWIYEKEFNQLW
                     RKANPKGYHEDLVKAKKQTRQNLALRDIGKYTKKYASTSYLFFKGLEKIAYSANLAKG
                     NFNQEAHRYASGLSNLKLFISPLISLFLIIFQANFSAAAQSKITETWLSASLPFIFAN
                     VVIGTSAAVVMIILQYGSEKRSKKGHIEAEQWLSFKKHLQDYKKTKNYAIDSIILWEK
                     YLVYGTLFGVSMKALSQLPVKFEDWSPTDRQLLVSYWGMSSINLSQSAVADLSNFNLG
                     SITHSLNSLGSAFNTISSGASRSYGASGGGSSGGFSGGGGGGGGGGGGGAG"
     gene            3050..5065
                     /gene="thrS"
                     /locus_tag="PLJ02_02355"
     CDS             3050..5065
                     /gene="thrS"
                     /locus_tag="PLJ02_02355"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOI05291.1"
                     /translation="MNQEEKLFALRHSAEHVLTLAMERLYAPNKKNQGKIKTASGEYK
                     LTKPAIVKAMGPAIDDGFYFDFDLSQEAAAAGFRLVEADLAKIEKEMGKLIAQNLLMQ
                     RLEVPIDLARAIFRDNPYKQELLDGLAELDAQKNKIDSASLRLDVERQQVEPGIVSIY
                     LTGKPEQIQADVQLLEGLKSNRLADAQSQIDLQSFVDLCKGPHVDQIGEIKAFKLLSI
                     AGAYWRGSEANKMLTRIYGTAFASQAELAKFLSNRQLALERNHRKIGKEMGLFAIINE
                     IGQGLPVWLPNGYAMRRAIEDYMIRFERSFGYTHVLTPHIQRKELFERSGHLGFYDES
                     MYPPLTYREEDDDSVADEVYYLKPMNCPAAMMIYKLQPHSYRELPMKIGEFGTVYRRE
                     KSGELHGLQRVRGFTQNDAHIFCTAEQLKGQFLEVMKMLEIFYRAVGFDNYKFRLSIS
                     DPDDKKFQVCGSPAEWQKAENTMRDILNEAGVEYEEVKGDAAFYGPKLDVQAINVFGK
                     EDSISTIQVDFNLPERFDVSYIDENGEKQRPFIIHRALIGSFERFFAFLIEYHGGDFP
                     LWFAPVQARLLPIADRHLDYAHSVMEKLLAAGVRVELDDRSERLQAKIRQAEADRVPL
                     MLIIGDQEVENKELSLRTRGRAQGLLKEGAIGLEEIIKIATALPALR"
     gene            complement(5170..>5540)
                     /locus_tag="PLJ02_02360"
     CDS             complement(5170..>5540)
                     /locus_tag="PLJ02_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOI05292.1"
                     /translation="EVEKGNIDFDVLLAAPELMPKLAKLAKILGPKGLMPNPKNGTLT
                     PKPELKKKELEAGTFTLKTENKAPLMHLVIGKVSMEDKTLEENLLAIIRALKNKIVKL
                     VITPSMGPAIRVNHQLEENK"
BASE COUNT         1628 a          965 c         1251 g         1696 t
ORIGIN      
        1 tgggttcttg agtctttgaa tcaacatcac taccaattcg ctcttacttg gtaatttgct
       61 taaagcttcg acttcagcaa cagataaaac tttatcttcc atgaagcctt gtttaatgct
      121 taatttgtct tgatttttgt gaaaatcaaa aaggactttt aaaccagcaa ttgcgtcttc
      181 ataagagaag acaatagcgt tcatgccagt taatgattca ctaactttgc ctttgcccaa
      241 agcaatatca atcagagtat ttttagtcac aaacatttcg ccaccagctt ttctgagagc
      301 gttgcgtaaa gttgttaaat cattgacact agtcccagca taatcaacga ttaccaaaga
      361 tttggcacga tcaattttgt cttgaatgat tttgacttgt tcgatattgt cttgacttgg
      421 cataaatcct ttttgtcgct ttttgtaata acaattaaaa ctagtggtta gccaaaaact
      481 ggagaaaggg gaaaaacatg atgcttttgc ttcctcggtg tgatgattaa gcctggtggc
      541 ccacactgtc tttggacaac agagcgatta taacagagaa tagttggctg acaaggcgca
      601 tttacaagca ataaactatc agtcataatg cgacctgatg agcaaacaaa ttgtaatggt
      661 taaaaaagtt ctaatcctgg taattttgtt atgtttccta acttcgagtt taagtttcag
      721 tcaaccagtt tcggccaata gttatgcaat tgatcgagca actattgaaa tttttttgca
      781 gaatgatggt tcagccaaag tgattgagca gcgggttttt aattttcaag gagattaccg
      841 ttttgtttat cacaaaattt tttatcaaaa tgatcaaact ttgtctgctg ttgggcgaag
      901 cgagccatat ttattaaaca attttgcact gtgtgaggtc gatcaaagta gtggggcaga
      961 tcaatataat tgctacgagc ttttagagca gaatcaggtt aatttgactg aaccacgcca
     1021 caatcccgac aatacttttt atgtcgaaca acgcgctaaa gaatattttg tgcaatggca
     1081 ctatcaggct atcaatgaaa aaaagatttt tgaactgcgc tatgaagtgg agaatttact
     1141 gactcaacaa ggcgatgttg ctgaattgta ttggcagttt gtgggcgatg cttgggaagt
     1201 agcgcatgga gctattgata ttacagtctg gttaccaact aattatttag cttcacaact
     1261 aagagatgtt caagcctggg gtcatggtcc tttggcaggt acagtcagga ttgatgcttt
     1321 gcccggtgat aatggtttat atggggttca ctttgaagca cctgccttgc cgatgaacca
     1381 gttcttagaa ggtcgagtga tttttcctct ggaagcttta ctagagacac aagctttgcc
     1441 cattggcaaa ttaaacagag agaagattat tgaacaagaa caagcctata ttgccgagac
     1501 tgcagctaag ttaaagagag aagaaacaat tgctaaggtt ttttttgctg gtgctttatt
     1561 gttgagtatc ttggaactgg tttggtttgt tcaaagttta atcaagcatt taaaattggc
     1621 taaagaagag cgcctcacag ctttacctgg agtgtgggag ccacctagcg atttagaacc
     1681 ggctttggtt tatcaactgt tgtataagac gaaaaaactc aatccggcag tttttactgc
     1741 cactgttttg tcgctagtgc ataaaaaagt gtgccgtatt gtccgtagcg agcaaaaaga
     1801 aggttttatt gtcaaggatt atcgttactt tctcgaaccc actgataaag tagggcaagt
     1861 gaagttgtca gaagttgaag aaaaggtctt tgaattttta tttaaagtgg ttggtggatc
     1921 ttatattcgt tattatcacc aggctagcgg taagtggatt tatgaaaaag agttcaatca
     1981 attatggcgc aaggccaatc ccaaaggcta tcacgaagat ttagttaagg ccaaaaaaca
     2041 aactcggcag aatttggctt taagagatat tggcaaatat accaaaaaat atgccagcac
     2101 tagctatctc tttttcaaag gtttagaaaa aatagcttat agtgctaatt tagccaaggg
     2161 taattttaat caagaagcac accgttatgc tagtggttta agtaatttaa aattatttat
     2221 ttctcctttg attagtttat ttttaattat ttttcaagca aatttttcag cagcggccca
     2281 gtcgaaaata actgaaactt ggttgagtgc ttctttacct tttatttttg ccaatgttgt
     2341 aatcggtacc agtgccgcag tagtgatgat aattttgcaa tacggtagtg aaaaaagaag
     2401 taaaaagggg catatagaag ccgaacaatg gttgtctttt aaaaagcatt tacaggatta
     2461 taaaaagacc aaaaactatg cgattgattc aataattctt tgggaaaaat atttggttta
     2521 tggcacttta tttggagtat cgatgaaggc tctttctcaa ttaccggtca aatttgaaga
     2581 ttggtcgcca actgatcggc aactgctagt cagctattgg gggatgtcga gcatcaacct
     2641 tagccaatca gcagtagcag acttgagtaa ttttaacttg ggtagtatta ctcattcact
     2701 aaattcattg ggcagtgctt ttaacacgat tagcagtggc gcatctcgta gttatggtgc
     2761 tagtggtggt ggttcaagtg gtggtttctc tggcggtggt ggtggcggag gtggtggtgg
     2821 cggtggtggt gccggttgat tttttaagag agcgaatttt cgtgttataa tgatcagcaa
     2881 tgttaaacat tatcttcact accaacacga ctactagccc ctagcgggtt gttgtgtgct
     2941 ggagtttaag ataattgacc acaaacgcag acccgcaaac aaggcgggtt tttttattgg
     3001 caattatcta aaaaattcat taaaataaga aagagacacg gaaggattta tgaatcaaga
     3061 agaaaaatta tttgccctac gtcatagtgc tgaacacgta ttgacgctgg ctatggagcg
     3121 actttatgcg cccaataaaa aaaatcaagg caaaattaaa actgccagtg gagaatacaa
     3181 gttaactaaa ccagcgattg tgaaggctat ggggccagcc attgatgatg gtttttattt
     3241 tgattttgat ttatcccaag aagcagcagc agcagggttt cgcctggttg aagccgactt
     3301 agctaaaatt gaaaaagaaa tgggcaagct cattgcgcaa aatttgctca tgcagcggct
     3361 ggaagtgcca atcgatttag ctcgggcgat ttttcgtgac aatccctaca aacaagaatt
     3421 acttgatggt ttagctgaac tggatgctca aaaaaacaag attgattcag cttcgttgcg
     3481 tttggatgtc gagcgacaac aggttgagcc tggtatagtg agcatttatc ttactggcaa
     3541 gcccgagcag attcaggctg atgtccaact actggagggt ttaaagtcaa atagattagc
     3601 agatgcccaa agtcaaatcg atttgcaaag ttttgttgat ttatgcaagg gtccccacgt
     3661 tgaccaaatt ggcgaaatta aagccttcaa attgttaagc attgctggtg cctattggcg
     3721 gggaagtgaa gccaacaaaa tgctcacgcg tatctatgga actgcttttg ccagtcaagc
     3781 cgaattggct aaatttttga gcaatcggca actggcgctg gagcgcaatc atcgcaaaat
     3841 tggcaaggaa atgggtttgt ttgctatcat caacgagatt ggacaaggac tgcctgtgtg
     3901 gttgcctaac ggttacgcca tgcgccgcgc aatcgaagat tatatgattc gttttgagcg
     3961 cagtttcggt tacacgcacg tgctgactcc tcatattcag cgtaaagaat tatttgaaag
     4021 atcaggacat ttaggatttt atgatgaaag tatgtaccca cctttaacgt accgagaaga
     4081 ggatgatgat tcggtggcgg atgaggttta ttatctaaaa ccaatgaact gtccagcagc
     4141 gatgatgatc tacaaattac aacctcacag ttatcgtgag ctgcccatga aaattggtga
     4201 attcggtacg gtttatcgtc gggaaaaatc tggcgagttg catggtctgc aaagagtgcg
     4261 gggctttacg caaaatgatg cgcatatctt ttgtactgcc gagcaactta agggtcaatt
     4321 tttggaagtg atgaagatgt tagaaatctt ttatcgagca gtcgggtttg ataattacaa
     4381 atttcgttta tccatttctg atcctgatga taaaaaattt caagtttgcg gttcaccggc
     4441 tgagtggcag aaagccgaga ataccatgcg cgacattctt aatgaggctg gcgtagagta
     4501 tgaagaagtc aaaggtgacg ctgcttttta cggtccgaag ttagatgtgc aagcaattaa
     4561 tgtttttggt aaagaagata gtatttcgac gattcaagta gattttaatt taccagaacg
     4621 ttttgatgtt agttacatcg atgaaaatgg tgaaaagcaa cggcctttta ttatccaccg
     4681 ggctttaatt gggtctttcg agcgcttttt tgctttctta atcgaatatc acggtggaga
     4741 ttttccctta tggtttgcgc cagtacaagc tagattattg cccattgctg atcggcatct
     4801 agattatgcg cactcggtaa tggagaaact tttggcagct ggggtcagag tagaacttga
     4861 tgatcgctca gagcggctgc aagccaaaat tcgtcaagct gaagcagatc gagtgccact
     4921 aatgttaatt attggcgatc aggaagtaga gaataaggaa ttgagtttgc gtactcgtgg
     4981 gcgcgctcaa ggtttactaa aagagggggc aatcggcttg gaagaaatta tcaagatagc
     5041 gacagcccta ccagcgttaa gataactagc gctaagctaa ccggtgaggc taattggtgc
     5101 taagctaaag taaaaataaa cccagccata tcataggctg ggtttatttg attatgacta
     5161 aatggtgacc tatttgtttt cttctagctg gtgattcact ctgatggccg gacccattga
     5221 tggggtaatc actaatttaa caattttgtt tttcaaagcc cgaataatgg ccagtaggtt
     5281 ttcttccagt gttttatctt ccatgctgac tttaccaatc actaaatgca ttagtggggc
     5341 tttattctcg gttttaagag tgaaagttcc agcctcaagc tctttctttt ttaactctgg
     5401 tttgggagtt aaagtgccat ttttggggtt aggcatcaaa ccttttggtc ccaaaatttt
     5461 agctaattta gccaatttgg gcatcaattc tggtgcagct aaaagcacgt caaaatcgat
     5521 attacctttt tcaacttcag
//
LOCUS       DAOSEW010000049         5357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_13581_length_5357_cov_4.491135, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000049 DAOSEW010000000
VERSION     DAOSEW010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13581_length_5357_cov_4.491135"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="PLJ02_02365"
     CDS             <1..464
                     /locus_tag="PLJ02_02365"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOI05293.1"
                     /translation="EVDGVQYFDDSIATNPTASSAALRSFPQGKIILLAGGSDKKLDL
                     TEYLESIIEQKVKALLLFPPVGPKILEQLQKLWEAKGLTADTFPQYRIFQSMAEAVQQ
                     ARTWAKAGDVVLLSPACASFGLFKDYQDRGQQFQLAVNNLITNQSTKSKSI"
     gene            501..1940
                     /locus_tag="PLJ02_02370"
     CDS             501..1940
                     /locus_tag="PLJ02_02370"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOI05294.1"
                     /translation="MNNLKNKLAPFVLSYLRFFAKLQLKKNSQAKIIGITGSAGKTSC
                     QSAVLAALGKNKARFKIKVSHKANSESGIPLDILGLRVKNYSPLDWLRLLLLAPWQLL
                     TNWQQFDLYIIEMGIDSPLPPKNMDYLLSIVQPQIAIILNAKAIHSANFDQLLNETEQ
                     KQREQAVIRLIAAEKSKLAQALPSSGLAIINRDDPAIWECSRQTQAEILTFGTAGGDA
                     EHELDLKLIDWQVNLAGSHFIFEIRNHLKKYNSGSRLLELNLKNLVLGKHYSAALASA
                     ILLAFKLGLKKNEIKNNLEKYWTLEPGRSSLLVGKNNSYLLDSSYNASGMLEMIELTQ
                     QLISNDQHIKRALAIIGDIRELGAETQAIHQEVAQALAESFSEVYLVGPLMENYALPI
                     LQDKIKNGAGQIKQVKTYLDSQSAGKDLAKNLAAGDLVLVKGSQNTIFLEEAVALLLA
                     EEKSAKKVLCRQSEYWLKLKKAETKQNGN"
     gene            complement(2008..3264)
                     /locus_tag="PLJ02_02375"
     CDS             complement(2008..3264)
                     /locus_tag="PLJ02_02375"
                     /inference="COORDINATES: protein motif:HMM:NF018435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOI05295.1"
                     /translation="MPKSLDSAKKSYKPSLWQRLDWRQALAIDLGSNEIRIFRANLRQ
                     KKQMEVLNIDQACVAIKRGSPSSQAKVLAIGRDALEMRERLGDEVEVIFPIIAGRIID
                     EKLAKLLLRELLKRALRVDRILPFVISPVVVVTVKAGTSEFSRRCLSRLFYDLGASEV
                     HLLLEPLAAAIGVGVPIADASGVMLMQMGAVGVEAAALSLGSVVSFQSNLWTGTVAGW
                     ELDELLRAYLVKKYHLLVTLEASESLKKRITLRASDALADDADNADGVGDDGSGDGGV
                     GDGGDTNHAQNRVKKELNKQDQDLPTKDLITLVGRDLISQKAKTITVSNQELVMAIKP
                     VIEDYIFLIERLMEEMPNALLVDVLDRGLLLSGNLAKLPGLDQFLAQALKMPVALVDD
                     PDLAVATGALTVLENLELFKASLAYA"
     gene            3337..4875
                     /locus_tag="PLJ02_02380"
     CDS             3337..4875
                     /locus_tag="PLJ02_02380"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOI05296.1"
                     /translation="MAKKKNNNILRNLIDSLKKIFKAIFTTRLGYSIFSLLVIVIGSI
                     FAIRYARGSWRLTEKGVVANTGLLNVNSFPTGAQVFINDRLVTATDDTIYIDPGEYQV
                     KIVKEGYSVWQKNLLVQEELVTQTNATLFPNAPSLSPLTFTGVNNIHPSPDGQKILFH
                     VSQANTPASNGFYLLELTSNFLSLQSGPRQITDDLPALELDKAHIIWSPDSQEVMILV
                     DEKFGGREFLIPIDKKSALAEQVDISFSRSKTLSAWEEEIYQKERLFLEKFPPEILQV
                     ATQSAKNVYISPDKKRLLYTATADVTLPDELIPPLPAASNQVEARDLKTGNIYVYDRE
                     EDKNFLVLNEAVRPNFPLRNLGTDGDNLNNEENAQKLDQTMSAENNNQQVELGFLNKQ
                     LLSDDLFATEARQLEASPEAFLRLQADAADDKETTAINFSNYYSGVSLNSLQWFPDSR
                     HLLYVADGKVQIMEYDGHNNTVIYSGPLIDEFVYPWPDGSQIIIYTHFSPDSPFNLYA
                     INLK"
     gene            4908..4981
                     /locus_tag="PLJ02_02385"
     tRNA            4908..4981
                     /locus_tag="PLJ02_02385"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4942..4944,aa:Pro,seq:cgg)
     gene            5053..5124
                     /locus_tag="PLJ02_02390"
     tRNA            5053..5124
                     /locus_tag="PLJ02_02390"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5086..5088,aa:Arg,seq:cct)
     gene            5173..>5357
                     /locus_tag="PLJ02_02395"
     CDS             5173..>5357
                     /locus_tag="PLJ02_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05297.1"
                     /translation="MAWNDFLTKLFAKKKHSSQQKKRIWTILLIVLPFLLGLVLYSLT
                     QVKEVAAQWWDETWLYRR"
BASE COUNT         1715 a         1147 c         1004 g         1491 t
ORIGIN      
        1 cagaagtaga tggagtgcaa tactttgatg actcaatcgc tactaacccg actgccagca
       61 gtgccgctct tcgcagtttt cctcaaggaa aaatcatttt gctagctggt ggttcagata
      121 aaaagttgga tcttactgaa tatctagaaa gtattattga acaaaaagtt aaagctttat
      181 tactgttccc accagttggc ccaaaaattt tagaacaatt acaaaaactt tgggaagcaa
      241 aaggcttaac cgcagatact ttcccccagt atcgaatctt tcagtcaatg gctgaggctg
      301 tccaacaagc gaggacttgg gccaaagctg gggatgttgt cttgctcagt ccagcttgcg
      361 ccagttttgg tttatttaaa gattatcaag atcgtggtca acaatttcag ttagcagtga
      421 ataatttaat aacaaaccag tcaacaaagt ctaaatcaat ttgaacgaaa cttaatctaa
      481 attaattgac attaaacacg atgaataatc ttaaaaacaa actcgctccc tttgtcctct
      541 cctacctgcg gttttttgct aaattgcagt tgaaaaaaaa tagccaggcc aaaattatcg
      601 ggattactgg gagtgctggc aaaactagtt gtcaatcagc tgtcttagct gctttgggca
      661 aaaataaagc tcggttcaaa attaaagtga gccataaagc taattctgaa tcgggcatcc
      721 ccctggatat tttagggcta agggtcaaga attacagccc tcttgattgg cttcgcttgc
      781 ttttactagc gccatggcaa ttactcacta actggcagca attcgatctg tatatcattg
      841 aaatgggcat tgacagtcct ctacccccca agaacatgga ctatttattg agtattgtcc
      901 aaccacaaat tgccatcatc ttaaatgcca aagctattca tagtgccaat tttgatcaat
      961 tgctcaacga aacagaacaa aaacaacgcg aacaagcggt aatcagactc attgctgcag
     1021 aaaaaagtaa attagctcaa gctttgccca gtagtggttt ggccatcatt aatcgtgatg
     1081 atccagctat ttgggaatgc agtcgacaaa ctcaagctga aattttaact tttggcacgg
     1141 ctggtggtga tgctgagcac gaacttgatc taaaactaat cgactggcaa gttaatttag
     1201 ctggtagcca ttttattttt gaaattagaa atcacctaaa aaaatacaat tcaggcagtc
     1261 ggctactaga acttaacctt aaaaacttgg ttttaggcaa acactacagc gcagctttag
     1321 ccagcgccat tttattagcc tttaaacttg gcttgaaaaa aaacgaaatc aaaaacaatt
     1381 tggaaaaata ttggactctg gaacctggaa gaagcagttt gctcgtcggt aaaaacaata
     1441 gttaccttct cgacagttct tacaatgctt caggcatgct agaaatgatt gagctgaccc
     1501 aacagttaat tagcaatgac caacacatta aaagagcact agccatcatt ggcgatatca
     1561 gagagttggg tgcagagaca caggccatac atcaagaagt ggctcaagcc ctagctgaga
     1621 gttttagtga agtctatttg gttggaccac tgatggaaaa ctatgcttta cccattttgc
     1681 aagacaaaat taaaaatgga gcaggtcaaa ttaagcaagt taaaacttat ttagatagtc
     1741 agtcagccgg taaagattta gcaaagaatc tagcagcagg tgatttggtt ttagtaaaag
     1801 gttcacaaaa cactattttc ttagaagaag cagtggcgct cttgctggca gaggaaaagt
     1861 cagcaaaaaa agttttgtgt cgacaaagtg aatattggct taagctaaaa aaagcggaaa
     1921 ctaaacaaaa cggaaactaa aaaacgcggc ggccaaacaa agcgaagatc aaacaaagta
     1981 ataaccaagc acacggccaa cccaaaatta agcgtaagct aaactagctt taaacaattc
     2041 caaattctcc aaaaccgtca gcgcccccgt cgccactgcc aaatcaggat catcgactag
     2101 cgctaccggc atttttagtg cttgggctaa aaactgatct aaaccaggta atttggctaa
     2161 attaccagac aaaagcaaac cccgatccaa aacatcaacc aacaaagcat tgggcatctc
     2221 ttccattaag cgctcaatta gaaatatata atcttcgatc actggtttaa tagccattac
     2281 caactcttga ttactaaccg taatcgtttt cgctttttgg ctgattaagt ccctgccaac
     2341 taaggttatt aaatccttcg ttggtaaatc ctgatcttgt ttgtttagct ctttttttac
     2401 tcggttttga gcatgattag tatcgccacc atcgccaacg ccaccatcgc cactaccatc
     2461 atcgccaacg ccatcggcat tatcggcatc atcagctagt gcatcactgg ccctgagagt
     2521 aatgcgtttt ttgagacttt cactggcttc taaagtaact aataaatggt attttttgac
     2581 taaataagcc ctgagtaatt catctaattc ccaaccagct actgttcccg tccacaggtt
     2641 actttgaaaa ctgactaccg aacccaaaga aagagcagcc gcctccacgc ccaccgcccc
     2701 catttgcatt aacatcaccc cactggcatc ggcaatcggg acgccgacac caatggcggc
     2761 cgctagtggt tccaacagta aatgcacctc gctagcgccc aaatcataaa ataaacgact
     2821 taaacagcgg cgtgaaaatt cactagtccc cgccttgacg gtgacaacta ccacaggact
     2881 gatgacaaag ggtaaaattc tatcgactcg caaagctctc ttgagtaact ccctcagtaa
     2941 cagcttagct aacttttcat caataattct gccagcaata atgggaaaaa tcacctctac
     3001 ctcgtccccc aatctctctc tcatttccaa agcatcccga ccaatagcca aaactttagc
     3061 ttgactcgaa ggactacctc gtttgatggc cacacaggct tgatcaatat tcagcacttc
     3121 catttgtttt ttttgacgaa gattggcccg aaaaatacga atttcattgc ttcctaaatc
     3181 aatcgccaaa gcctggcgcc aatccaatct ctgccacaat gagggtttat aactcttctt
     3241 agcagaatca agacttttag gcatctaatt gatataatag tcaaagacct cagcaagaac
     3301 aaggcacaaa aatattaatt aacagattct aatttaatgg caaagaaaaa aaacaacaac
     3361 atcctacgga acttaatcga ttcgcttaaa aaaattttta aggcgatttt tactactcga
     3421 ctaggttaca gcattttttc cttattagta atcgtgattg gtagcatatt tgccattcgc
     3481 tatgcccgtg gcagttggcg tcttactgaa aaaggtgtag tggccaacac tgggttgtta
     3541 aacgttaatt cattccccac cggtgcgcaa gtgtttatta atgatcggct agttaccgcc
     3601 accgatgaca ccatttacat tgatccaggt gaatatcaag tcaaaattgt taaagaaggt
     3661 tattcagtct ggcaaaaaaa tctgttggtt caagaagaat tagtgacaca aactaacgcc
     3721 actctcttcc ctaacgcacc tagtctctcc cctttaactt tcactggggt gaataatatc
     3781 catccttctc ctgatggaca aaaaattctt tttcacgtta gtcaagctaa taccccagct
     3841 agcaacggtt tctatctttt agaactaacc agtaattttt tatctctgca aagtggcccg
     3901 cgccaaatta ctgacgatct gccggctcta gaattagata aggcacacat catttggtca
     3961 ccagatagtc aagaagttat gattttagtt gatgaaaaat ttggtggtag agaatttttg
     4021 attccaatag ataaaaaaag tgctttagcc gaacaagttg acatttcttt ctcaagaagt
     4081 aaaactctat cagcttggga agaagaaatt taccaaaaag aacgactatt cttggagaaa
     4141 tttcctccag aaattttgca agttgccact cagtcagcta aaaatgttta catctctcca
     4201 gacaagaaaa gattactcta cacggctaca gccgatgtca ccttaccaga tgagttgatt
     4261 cctcccctgc ctgctgccag caaccaagtt gaagcaagag atctaaaaac tggtaatatt
     4321 tatgtttacg atcgggaaga agataaaaat tttttggtgt taaatgaggc agttcgccct
     4381 aatttcccct tacgaaactt aggaactgat ggcgacaatt taaacaatga agaaaatgcg
     4441 caaaagcttg atcaaacaat gagtgcagaa aacaacaatc agcaagttga gcttggtttt
     4501 cttaataaac aactactcag tgatgattta tttgcaactg aagcccgaca attagaagct
     4561 tctcccgagg cttttttgcg tttgcaagca gatgcggctg atgataaaga aacaacggct
     4621 attaatttta gtaattatta ctcaggagtc agtttaaaca gtctccaatg gtttcctgat
     4681 tccagacact tactttatgt agccgatggc aaggttcaga ttatggaata tgatggccat
     4741 aataacacag tcatttattc tggacctctt attgatgaat ttgtttaccc ctggccagat
     4801 ggtagtcaaa ttatcattta cactcatttt agtccagatt cgccctttaa cttgtatgcc
     4861 attaatttaa aataagccac ggcaaggcaa cagagttcag tacaaaacgg agattagctc
     4921 agatggctag agtgcggcgt tcgggacgcc gaggtcggcg gttcgagtcc gccatctccg
     4981 acttggatag gccctgatat aataatgcga aatttggcct tgtgagaaaa tgtagctttg
     5041 tgggaaaatt tggcctccat agttcaacgg atagaacgaa ggtttcctaa accttaaatc
     5101 caagttcgat tcttggtggg ggcacccaat actagctgat atatgatttt ttctttatac
     5161 ttaacttaag ttatggcttg gaatgatttt ttaactaaac tttttgctaa gaaaaagcac
     5221 tctagtcagc agaaaaaaag aatatggaca attttattaa ttgtgcttcc ctttttactg
     5281 ggattagttc tctatagttt aactcaagtt aaagaagttg cagcgcagtg gtgggatgaa
     5341 acttggttat atcggcg
//
LOCUS       DAOSEW010000050         5257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_13959_length_5257_cov_3.727989, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000050 DAOSEW010000000
VERSION     DAOSEW010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13959_length_5257_cov_3.727989"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="PLJ02_02400"
     CDS             complement(<1..1166)
                     /locus_tag="PLJ02_02400"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOI05298.1"
                     /translation="MGYKQYAIAGFSIQSLQKAFIFFLNFLKITILARLLKPTDFGLF
                     SLVMIAIGLTESFTQTGINTTIVQSKKDINYFLDTAWVIAIIRGFLIGVIMLVLAFAM
                     SNFYQEDLLLPLITVATLIPIIKGFINPAIAQWQKNFRFAKEGLYHAIHLTAEVLMQI
                     VLALIWKSVWALLGGVILAAVVEVIVSFIMNPQRPKFHYRSDRARHIFSNSKPLMLAS
                     VLTYLNDNADDFLIGKMLDAHQLGIYHNSYSLSHKLNYELSKSAHHGLMPVFSNLHKE
                     DEQPRLRRAFKSSIFSTFLLSLALSLPLIIWPSFFVNLILGDQWLEASQIIGILVLAG
                     LVHSLTNIVYALQIAQKRYLPMNFHLLVSLLLMIAGIISLTPTYGLYGACLGIL"
     gene            1234..1824
                     /gene="lepB"
                     /locus_tag="PLJ02_02405"
     CDS             1234..1824
                     /gene="lepB"
                     /locus_tag="PLJ02_02405"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOI05299.1"
                     /translation="MATKFLSKIAGGFLDLVETVAISLSIFLVIYLFLMQPHQVNGQS
                     MLPNFQDGEHVMTDKISYKFREPMRGEVVVFHAPPAAGCVEGTGCDFIKRIIAVPGDK
                     VAVHDNAIWINGQKLPEPYIPDDFEILPGRATLNEEIYLGPDEYFVSGDNRPHSSDSR
                     FWGPITKDEIVGRVFLRYWPISEIELIKKAEYPAGF"
     gene            1906..2892
                     /gene="murB"
                     /locus_tag="PLJ02_02410"
     CDS             1906..2892
                     /gene="murB"
                     /locus_tag="PLJ02_02410"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOI05300.1"
                     /translation="MTSNQRLLKTEFPDIEWSFDLPLASLSYFKLGGPAEIFYKVTDT
                     ELLKRILVFCHHAAIPWTVVGGLSNVIIADAGVKGVVLQLATKGFKILEDTTEQLLVE
                     VEAGMKTNQLVSKSTYLNATGLEGFIGVPGRVGGAIYNNAHYLQHLIGDYIEKVVSFH
                     VKNNQEMIFSHENCKFAYEHSIFQEDKQLVILSATFLLKKDQPVNIRKKLVEAQTRRV
                     SSQPLNLPSSGCIFQNPRNSDYLKQLFPQFANQEFIPAGFLIDQAGLKNSREGAFVVS
                     DKHAAFVVNKAEILAIPASTKDLKKLIKKIQTEIKNKFQVELKEEVFYLGQD"
     gene            2907..4256
                     /gene="murA"
                     /locus_tag="PLJ02_02415"
     CDS             2907..4256
                     /gene="murA"
                     /locus_tag="PLJ02_02415"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOI05301.1"
                     /translation="MNSDTTATFHIQGSTPLHGSVRVGGAKNAAYKLMIASLLASSES
                     RLLNLPDIADVDMVKKIINELGARAYSAGEHTLFIDPQNFRFSHVDGQYGKRSRASSL
                     FLPVLLAKFGEAAVPLPGGDQIGKRPLERHLQGLEKMGVVFSQNGDMLEARINTNQPK
                     KRLKACHYTFEKNTHTGTETLIMAAALAEGKTLLENAALEPEIDDLIAFLNEMGAKIR
                     RRSFRKIEIEGVDKLTGAIHRVMPDRNEAVSYACAAIITRGDIVVENARHRDLTAFLE
                     KLDEVGAGYEIGNYGIRFFYKGALQATDIETDIHPGFMTDWQPLFATLLCHCHGESII
                     HETVMQSRFQYAEHLRQMGAKIEYFNPVVKNPEKVYNFNWSDNRPEDFHAIKITGPVD
                     FIGGEFTVHDLRAGATILLAAIAAKGKTVLRNIEQIERGYQDIEEKLVSMGANIERR"
     gene            4234..>5257
                     /locus_tag="PLJ02_02420"
     CDS             4234..>5257
                     /locus_tag="PLJ02_02420"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05302.1"
                     /translation="MPTSKGDEMGSIVLFLGILLFSFIVTSMAVVPFINLLYKLKFRR
                     AQQQTKDAFGKRTPIYDKFHKNKAGVPVGGGLLIVVLVSVFFALMIPLLNYFGIEVTS
                     LHQNSQVEINVLFFTFLSFAILGLYDDLKKIFSFEKTKFFGLRLRQKLLLQIILAVII
                     SLMLHFQLGISILHIPFVGTFDLGWGYIPFATFVIVAFTNAVNITDGLDGLASGLLMI
                     GLFGLWILSSSILDVPLSVFIALWLGSLISLLYFNIYPARLFMGDVGSLAFGATLAVI
                     GLLLGKVFTLLIIGFIFVLEIMSSLLQLLAKRYWKRKILPAAPLHLTLQERGWDEPKI
                     VQRAWLI"
BASE COUNT         1580 a          921 c         1116 g         1640 t
ORIGIN      
        1 aataaaatac ctaaacaagc accatagagg ccataagttg gggttaaact aataatgccg
       61 gcaatcatta acagcaagga aactaagaga tgaaagttca ttggcaaata ccttttttga
      121 gcaatttgta gtgcgtaaac aatgtttgtc aggctatgaa ctagtccagc tagcactagg
      181 ataccaatga tttgactggc ttcgagccac tggtcgccca aaattaaatt gacaaagaaa
      241 ctgggccaaa taattagtgg taagcttaga gctaaactaa gcaaaaaagt gctaaaaatt
      301 gaacttttaa aagcccgcct taatcttggt tgttcatctt ctttgtgtaa attggaaaaa
      361 actggcatta aaccatgatg agctgattta gataactcat aattaagttt atgacttaag
      421 gaataggaat tgtgataaat acctaattgg tgagcatcaa gcattttacc aatcagaaag
      481 tcatctgcat tgtcattaag ataagttaat acactagcta acatcagcgg tttactattg
      541 ctgaaaatat gacgagctcg gtcacttcgg tagtgaaatt ttggccgctg tggattcatg
      601 ataaaagaaa caataacctc aaccactgcc gctagaatta ccccgcccag taaagcccaa
      661 accgacttcc agatcaaagc tagcacgatc tgcatcagca cttcagcggt aagatgaatg
      721 gcatgatata acccttcttt ggcaaagcga aaatttttct gccattgagc aatggctgga
      781 ttgataaaac ctttaatgat cggaattaag gtggcgacag tgattagggg aagcaataaa
      841 tcctcttgat aaaaattgct catagcaaag gctagaacca acataatcac accaattaaa
      901 aaaccacgga taatcgcgat cacccaggcc gtatcaagaa aataattaat atcttttttg
      961 gattgaacga tagtagtgtt aatgcctgtt tgggtaaaac tctccgttaa accaatggca
     1021 atcatcacta aagaaaagag gccaaagtca gtcggcttga gtaatctggc caaaatcgtg
     1081 attttcagga aattaagaaa aaagataaaa gctttttgta gactctgaat agaaaagcca
     1141 gcaatagcat attgtttata acccatggct accattataa aacaatcttt tgctcttgcc
     1201 agctagtgat catcagcgtt aaaatgaaaa actatggcaa caaagttcct cagtaaaatt
     1261 gctggcggtt ttttagattt agtagagaca gtagcaatct cgttgtctat ttttttggtg
     1321 atttatttgt ttttaatgca accgcaccag gtcaatggtc aatcaatgct ccccaatttt
     1381 caggatggcg agcacgtgat gactgataaa atttcctata agtttcgtga accaatgcgg
     1441 ggtgaagtgg tggttttcca tgctccgcca gctgcaggat gtgtggaagg tactggctgt
     1501 gattttatca aaagaattat cgctgttcct ggagataaag tcgctgtgca tgacaacgcc
     1561 atttggatta atggtcaaaa attaccagaa ccctatattc ccgatgattt tgaaatttta
     1621 cctggccgag cgactttgaa tgaagaaatt tatttaggtc cagacgaata ttttgtatct
     1681 ggtgataatc gaccgcactc aagtgattcg cgtttttggg gaccaatcac taaagatgaa
     1741 atagtgggtc gtgtttttct ccgctattgg ccaatcagtg agatagagct aattaaaaaa
     1801 gcagaatatc cagccggttt ttaatttttt gtttcttttt tatttgcaat tattttggat
     1861 attgctcttg agcaattgct caaaaaacaa gtacaatagc taataatgac aagcaatcaa
     1921 cgtttactaa aaacagagtt tccagatatt gaatggtctt ttgatttgcc gctggccagt
     1981 ctcagttact ttaaattagg tggtcctgca gagatttttt ataaagtgac cgatactgaa
     2041 ttattaaaga gaattttagt tttttgtcat cacgctgcca ttccttggac ggtggttggt
     2101 ggtttaagta atgtaattat tgctgatgcc ggagttaagg gcgtggtgtt gcagctggct
     2161 actaaaggtt ttaagatctt agaagatacc acagaacagc tgcttgtcga ggtggaagcc
     2221 ggcatgaaaa ctaatcaatt agtgagtaaa tcaacctatt taaacgccac tggtttggag
     2281 ggttttatcg gggtacctgg tagagtgggt ggagccattt acaataatgc tcattattta
     2341 cagcatctaa taggagacta tattgagaaa gttgtatctt ttcatgtgaa aaataatcaa
     2401 gagatgatat tttcacatga aaattgtaaa tttgcgtatg aacattcgat ttttcaagaa
     2461 gataaacagt tggttatttt gtcagctact tttctgttaa agaaagacca accagtcaac
     2521 attagaaaaa aactggtcga agctcaaacc agacgagttt cctctcaacc gctgaattta
     2581 cctagttctg gttgtatttt ccaaaatcct cgaaactccg attatctaaa acaactgttt
     2641 ccccaatttg ccaatcaaga atttattccg gctggttttt taattgatca agcaggctta
     2701 aaaaattctc gcgaaggcgc ttttgtggtt agtgataaac acgctgcctt cgtcgttaac
     2761 aaggctgaga tattagcgat tcctgcttca accaaggatt taaagaaatt aattaaaaaa
     2821 attcaaactg agataaagaa taaatttcag gtagaattaa aagaagaagt cttttattta
     2881 ggtcaagact aaaagagaaa gtttagatga actcagacac cacagccact tttcatatcc
     2941 aaggtagtac cccactccat ggtagtgtgc gggtgggtgg agcaaaaaat gctgcttaca
     3001 aattaatgat cgcttctctg ctcgctagta gtgaaagtcg tttgttaaat ttgcctgata
     3061 tagcagacgt ggatatggtg aaaaagatta tcaatgaact gggtgcgcgc gcttatagcg
     3121 ctggtgagca caccttattt attgatccac agaattttcg ttttagccat gttgatggtc
     3181 agtatggtaa acgctctcga gcctcatctt tgtttttgcc agtgctgtta gcaaaatttg
     3241 gtgaagcagc tgttccgctt ccgggaggag atcagatagg taaaagacca ctagaacgac
     3301 atctgcaagg tctcgagaaa atgggggtag ttttttccca aaatggcgac atgctagaag
     3361 caagaatcaa tactaaccag ccgaaaaaaa gactgaaagc ttgtcactat acttttgaga
     3421 agaatactca tactggtact gaaactctga ttatggcagc ggctttggcg gaaggtaaaa
     3481 ctttactgga aaacgcagcc ttagagccag aaattgacga cttaattgct tttttaaatg
     3541 agatgggtgc gaaaatccgc cggcgctctt ttcgcaaaat tgagatcgag ggagtcgaca
     3601 agctcactgg agcaattcat cgggtgatgc ccgatcgcaa tgaagcagtg agttatgctt
     3661 gtgcggcgat tattacgcga ggtgatattg tagtagaaaa tgcccgtcat cgagatttaa
     3721 ctgcctttct agaaaaactt gatgaagtgg gagctggtta tgaaattggt aactatggta
     3781 tccgcttttt ttacaaagga gctctccaag cgacggatat tgaaaccgac attcatcctg
     3841 ggtttatgac tgattggcag cctctgtttg ccaccctact ctgtcattgt catggtgaaa
     3901 gtattattca tgaaacagtg atgcaaagcc gtttccaata tgctgagcat ctcagacaaa
     3961 tgggcgccaa aatcgaatat ttcaacccag tagtgaagaa cccagaaaaa gtttataatt
     4021 ttaattggtc ggacaatcgt ccagaggatt ttcacgccat taaaattacc ggtccagttg
     4081 attttatcgg cggtgagttt actgtccacg atttgcgagc cggagctacc attttgttgg
     4141 cggcgattgc agctaaaggg aaaactgttt taaggaatat tgaacaaatt gagcgcggtt
     4201 accaagacat tgaagaaaaa ttagtgtcaa tgggtgccaa catcgaaagg agatgagatg
     4261 gggtcaatcg ttttattttt agggatttta ttattttctt ttattgtgac tagtatggcg
     4321 gtagtgcctt ttattaattt actttataaa ctcaagtttc gtcgagctca gcagcaaacc
     4381 aaagatgctt ttggtaaacg aacgccaatc tatgataagt ttcataagaa caaagcggga
     4441 gtaccagtcg gtggtggttt gttaattgtg gtcttggtgt cagttttctt tgcgctgatg
     4501 attcctctct taaactattt tggcattgaa gtgacatcac tgcaccaaaa tagtcaagta
     4561 gaaattaacg ttttgttttt caccttttta tcgtttgcga ttttgggttt atatgacgat
     4621 ctgaaaaaaa ttttttcttt tgaaaaaact aaattttttg gtttgagact aagacaaaaa
     4681 ttacttttac aaatcatttt ggctgtaatt atttcgctaa tgcttcattt tcaattgggt
     4741 atttcgatct tacacattcc gtttgtcggt acttttgatc tcggttgggg ttatattccc
     4801 tttgctactt ttgtcattgt ggcttttacc aacgctgtca acattactga tggtctggac
     4861 ggtttagctt caggcctact gatgattggt ttatttggtt tgtggatttt atcttcgagt
     4921 atcttggatg ttcctttgtc ggtttttatt gccctgtggt taggctcatt aatttctttg
     4981 ctttatttta atatttaccc ggcgcgttta tttatgggcg atgttggctc cttggctttt
     5041 ggagcgacct tggcggtcat tggcttgctg ttaggtaagg tctttacttt gctaattatt
     5101 ggttttattt ttgttttgga aattatgagt tcacttttac aacttttagc taagagatat
     5161 tggaaaagaa aaattctgcc agctgctcct ttacatctga ccctacaaga acgggggtgg
     5221 gatgagccaa aaattgtgca acgagcttgg ctgatac
//
LOCUS       DAOSEW010000051         5216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_14116_length_5216_cov_4.364852, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000051 DAOSEW010000000
VERSION     DAOSEW010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 5216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5216
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14116_length_5216_cov_4.364852"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /locus_tag="PLJ02_02425"
     CDS             complement(<1..1119)
                     /locus_tag="PLJ02_02425"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOI05303.1"
                     /translation="MPSQNLPKPNVPGQTNDQGQANPSAKVTSVFPKTTPGISSRENP
                     AGRPPAKPTLTPNQPLTQPGDLSQSQTISPIASATSRPTTPATKMAMPKDLNDKEQKI
                     QMPQPFVPSEIQSTAKPVIAPAAKVLTTDAGVSTEDLELTQPTSAPQPNSANDQPTVD
                     TKDLKNGKNVQPPKKKGKFPLPKPILILLLLLVVGLIGFVVFRTFFAGGAKPTPVPSG
                     PNQEVGQPKEIINITYWGLWEDGEILNEVFKDFEAKEGIRVDYRKQSHRDYRERLEQA
                     LISGSGPDVFRYHITWVPMLSSQLAPMPNSVMTSAEYQQTFYPIAYEGLQTNGGIVGL
                     PLMYDGLALFYNKELLANANLSVPTTWSELRGVANQLTV"
     gene            1169..3016
                     /locus_tag="PLJ02_02430"
     CDS             1169..3016
                     /locus_tag="PLJ02_02430"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOI05304.1"
                     /translation="MDKILPAIKTSFHLFKRNLYLLLLGALLLFGVIILSKIYINYYQ
                     ERFYPRTYIDHVNVGGLSKTEAEQKLASNWSTASKQVPLQIIYDPNLIEETSIKLTLQ
                     DNFTQTLEQALSYAKQQNFFKALPKIWQRKDFYTQATINEEEMLKALAELAERFDLDQ
                     QTASATLKQSGNPQSLVINPGAAGRQLHIGKSREKIKAVLTTPWLELETEDLLVAAVV
                     ASTSAQLDQEQVEAAYARAQQLVGQNLVFSGEQQRVTFSDQELINILSFPDGINEELF
                     DEEGLIKLIEQKTSQINRPAQNASFQFHRNEAGQIIVDQFIPDLKGLTVDRSNLKNKI
                     IEILLALENGQNLEEIQANDPNVFQLAMESQDADITLAQTNDFGINELIGFGESWYYG
                     SIASRVYNVDLTNKILNWTVVEPGEEFSFNQALGDVDTSMGYREAYIIKDGATQLAAG
                     GGVCQTSSTLFRALLDAGVKITRRLPHSYRVSYYEINNDPGFDATVYAGDVDLRFIND
                     TPGHLLLEFNNDSEALYMTVKIYGTNDGRSTEIVNYRKWGYQPAPEPVYIPDPNLPAG
                     TVKQIDWASSGIKTEFTNVIKNQAGEVIREDYYYSNYQPWSAKYLQGIQ"
     gene            complement(3139..4869)
                     /locus_tag="PLJ02_02435"
     CDS             complement(3139..4869)
                     /locus_tag="PLJ02_02435"
                     /inference="COORDINATES: protein motif:HMM:NF020075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOI05305.1"
                     /translation="MSKRLFSLRFFALGLISLLGTLSFVFANSILFGSPQVVASEVDD
                     LQKEIDELAHLKKLSEDATTPLEAEVANLEARIRSAQAGINNAKQEAAQLAVDIEQRE
                     KDLAYHYQLFANRIAESYKRMRSFNPLALIFASANATVLSKNLAYQDSAKAQDDRLIR
                     SISEELKQLADDKRELEETQTRLAALQIQLDEQADFFKGEIDKAKDYQQTLGTKIAEL
                     SVRQQAILSEKSGTFQTTVGDVPLADDPASRPDYSPGFSPAFAVFSFGAPHFKGMSQY
                     GAFGRAKSGQNYQEILRAYYGGGIEIRDHDQNAQIYVTDFGISYTLEEYVKRIYEMPS
                     SWGDQGGMEALKAQAVAARSYALARGGSICATEACQVVKREPKGGNWEVAVNATAGKV
                     VYANGQPFSTWYASTSGGYQESYTSNGYSTPGFWDTPNGRSGWTSAAYENIAGSPWFY
                     KAWYKTRSGDACGRNNPWLTSEEMADIVNAWVVMYEGGGDSSRVTPESSCWGGNPYSK
                     AELVAIGGYTSVSGASVQYAENGVTATVTLQTNKGSKTIAGTEFYKVFNLRAPARISV
                     KSGLFNIEKK"
     gene            4935..>5216
                     /locus_tag="PLJ02_02440"
     CDS             4935..>5216
                     /locus_tag="PLJ02_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05306.1"
                     /translation="MKSKLLKIFQVFLVTISFLFLKNQKILAAEIDCGTYMYRASTGQ
                     FDSDCKCEHAEFAKIEVGFLGGVEKWCCGWYNKTEDVCQAEPTESDAAYE"
BASE COUNT         1590 a         1096 c         1033 g         1497 t
ORIGIN      
        1 aacagttaat tgattagcaa caccacgcaa ctcagaccaa gtagttggca ccgataaatt
       61 agcattggca agcaattcct tattataaaa aagcgctaaa ccatcataca tcagaggcag
      121 gccaacaatc ccaccattag tttgcagacc ttcataagca attggataaa aagtttgttg
      181 gtattcagca ctagtcataa cgctattggg cataggtgct aattgactcg acagcattgg
      241 cacccaagtt atgtgataac ggaacacatc tggaccacta ccagagatta gtgcctgttc
      301 taaacgctct cgataatcac gatgtgattg tttgcggtaa tcaacgcgaa taccctcctt
      361 agcttcaaaa tctttaaaga cttcgtttaa aatttcccca tcttcccaca aaccccaata
      421 ggtgatgtta ataatttctt taggttggcc aacttcttga ttcgggccac tgggtacagg
      481 cgtaggtttg gcaccaccag caaaaaaagt tctaaaaact acaaaaccaa tcaaccccac
      541 aactaaaaga agcaaaagaa ttaagattgg ctttggtagt ggaaatttcc cttttttctt
      601 gggtggctga acatttttac catttttaag gtctttagta tcaactgttg gttgatcatt
      661 ggctgaattt ggctgaggag cactggttgg ttgagttaat tctaagtcct cagttgatac
      721 cccagcgtca gtcgttaaaa cctttgcagc gggagcaatg acgggcttag cagtagactg
      781 gatttcgcta ggaacaaaag gctgaggcat ctgtattttt tgttctttgt catttaaatc
      841 ctttggcatt gccatcttgg tggccggggt agtcgggcga gaagtagctg aagcaatcgg
      901 agaaatcgtt tgagattgac tcaaatcacc tggttgagtg agtggttgat tgggagtcaa
      961 agtaggtttg gctggtggtc gcccagctgg attttctcta gaggaaatac ctggagtagt
     1021 cttaggaaag acagaagtaa ctttggctga gggattagct tgaccctggt cattcgtttg
     1081 acctggaacg ttaggtttgg gtagattttg acttggcata aaaatttaaa agcactacaa
     1141 tcaataattg taaagctaat agcctattat ggataaaatt ctgccagcaa tcaagacgag
     1201 ctttcacctt ttcaaaagaa acttgtattt gctactttta ggcgcattgc ttttatttgg
     1261 agtaataatt ttaagcaaaa tttatattaa ttattaccaa gaacgttttt atccaagaac
     1321 ttacattgac catgtaaatg tgggaggatt gagcaaaaca gaggccgaac aaaaactggc
     1381 gagtaattgg tcgacagcta gtaagcaagt gccactacaa attatttatg atcctaattt
     1441 aattgaagag acaagcatta aactaacttt gcaggataat tttactcaga ccctggaaca
     1501 agcgctgagt tatgcaaaac aacaaaattt ctttaaagcc ttaccaaaga tttggcaaag
     1561 aaaagacttt tatactcagg ccacaatcaa cgaggaagaa atgttaaagg cgctggctga
     1621 attagccgaa cgcttcgacc ttgaccaaca aacagccagc gccacactca agcaatctgg
     1681 taacccccaa tccttggtaa ttaatccagg agcagctggt cgccaattac acataggtaa
     1741 aagccgagag aaaatcaaag cagttttgac caccccctgg ctggaattgg agacagaaga
     1801 cttgctagta gcagccgttg tcgcctcaac tagcgctcaa cttgatcaag aacaagttga
     1861 ggcagcctat gctcgagccc agcagctagt tggccaaaat ctagttttta gtggcgaaca
     1921 gcaacgggtt acatttagcg accaagaatt aattaatatt ttaagttttc ctgatggaat
     1981 taatgaagaa ttatttgatg aagaaggctt aatcaaacta attgagcaaa aaacatctca
     2041 gatcaatcga cccgcccaga atgccagttt tcaatttcat cgcaacgagg caggacaaat
     2101 catcgttgat caatttattc ctgacctcaa aggtttaacc gtcgatcgtt ccaatttaaa
     2161 aaataaaata attgagatcc ttttagcctt ggaaaatgga caaaatcttg aggaaattca
     2221 agctaatgat cccaatgtct ttcagttggc aatggaaagt caagatgctg acatcacttt
     2281 ggcgcaaacc aatgattttg gcatcaacga actcattggt tttggagaaa gttggtatta
     2341 tgggtcaatt gccagtcgcg tttacaatgt tgacttaacc aacaaaattc ttaattggac
     2401 cgttgttgaa cctggtgaag agttttcatt taatcaggca ttgggcgatg ttgatactag
     2461 tatggggtat cgtgaggcct acattattaa agatggcgcc acgcaactgg ctgccggtgg
     2521 cggagtttgc caaacaagta gcaccttatt tagagcactg ctcgatgctg gcgtaaaaat
     2581 tactagacgt ctaccccact cttaccgcgt cagttattac gagattaata atgatccagg
     2641 ctttgatgcg acggtttatg ctggcgatgt tgatttgcgt tttattaatg acacgccagg
     2701 tcatctctta ctagagttta acaacgactc tgaggcttta tacatgactg tcaaaattta
     2761 tggtaccaat gacggacggt caaccgaaat cgttaattat agaaaatggg gctaccagcc
     2821 ggcacccgaa ccagtctaca ttccagatcc caatcttcca gctggcacag ttaagcaaat
     2881 tgattgggca agttcgggaa ttaaaactga atttactaat gtcatcaaaa accaagctgg
     2941 tgaagtcatt cgcgaagatt actactacag taactatcaa ccatggagtg ctaaatattt
     3001 gcagggaatt caataaaatt agtaaagcat tccccaactt tgcttgtaat tttataacct
     3061 tgttggaaat ttgataacct tgatattcta cttgatattc tatgaatggt tattttttcg
     3121 ttttcttcaa ttaattaatt attttttctc aatattaaac aagccagact taaccgaaat
     3181 cctagctggt gctcgcaaat taaacacttt ataaaactct gtcccggcaa tagtcttact
     3241 ccctttattg gtttgtaaag taacagtggc agtgacccca ttttctgcat attgcacact
     3301 tgccccactg acactggtgt aaccaccaat cgctactaat tcggcttttg aatagggatt
     3361 gccaccccaa caagaagatt cgggagttac tcggctacta tctcctccac cctcatacat
     3421 taccacccac gcgttaacaa tatcggccat ttcttcacta gttagccagg gattattccg
     3481 accacaagca tcaccagatc gggttttata ccaagcctta taaaaccaag gcgaaccagc
     3541 gatgttttca taagcagcac tagtccaacc actacgacca ttaggtgtgt cccaaaaacc
     3601 cggagtgcta taaccattgc tagtgtatga ttcttgataa ccaccagacg tagaagcata
     3661 ccaagtcgag aagggctggc cattggcata aactactttg ccagcagttg catttactgc
     3721 cacttcccaa ttgccacctt ttggctcacg tttaaccacc tgacaagctt ctgtggcaca
     3781 aatcgaccca cctctggcta aagcataact gcgagcggcg actgcttgag ctttaagagc
     3841 ttccatacca ccctgatcgc cccaactact cggcatctcg taaatgcgct tcacgtattc
     3901 ctccagtgtg taagatatgc caaaatcagt cacataaatt tgagcatttt gatcatgatc
     3961 tctaatttct atacctccac cataatatgc tcgcaggatt tcttgataat tttgtccgct
     4021 ctttgctcga ccaaaagcac cgtattgact catcccttta aagtgtggcg caccaaaact
     4081 aaaaacagcg aaagctggtg aaaagccagg actgtaatca ggccgactgg cagggtcatc
     4141 ggccaaaggc acatcgccaa ccgtagtttg aaaagtgcca gacttttccg ataaaattgc
     4201 ttgctgtcta actgataatt ccgctatttt ggtccccaat gtttgttggt aatccttggc
     4261 tttgtcaatt tcacccttaa aaaaatcagc ttgctcatct aattgaattt gtaaagccgc
     4321 caaacgagtt tgcgtttctt ctaactcccg cttgtcatca gccagttgtt ttaattcttc
     4381 agaaatagag cgaattaaac gatcgtcttg agccttagcg gaatcttgat aggctaaatt
     4441 tttactcaat acagtagcat tagcactagc aaaaattaag gctaaaggat taaaactacg
     4501 catccgctta tagctttctg caatgcgatt agcaaataat tgataatgat atgctaaatc
     4561 tttttctctt tgctcaatat ctactgctaa ttgcgctgct tcttgtttag cattattgat
     4621 tccagcctga gccgaacgaa tacgggcttc caaatttgct acttcggctt ccagaggagt
     4681 agtggcatct tcagatagtt tttttagatg agctaactca tcaatttcct tttgcaggtc
     4741 atccacttca ctagcaacaa cctgtggaga gccaaacaaa atactattgg cgaaaacgaa
     4801 agaaagtgta cccaataaac taattagccc aagcgcaaaa aatcgtagcg aaaataatcg
     4861 tttagacact ctttcattgt aacaaatttt tctattgctt ttgtgattga gatttttgag
     4921 gtaaaataaa gctcgtgaaa agcaagctgt taaaaatctt tcaagttttt ttggtaacaa
     4981 ttagtttcct ttttttaaaa aatcaaaaaa ttttggcggc cgagattgac tgcggaactt
     5041 acatgtacag agctagtacc ggacaatttg actcggactg caagtgtgaa catgccgaat
     5101 ttgccaaaat tgaagttgga ttccttggag gagtagaaaa atggtgttgt ggttggtaca
     5161 acaaaacaga agatgtttgt caagcagaac caaccgaaag tgatgcagcc tatgag
//
LOCUS       DAOSEW010000052         5122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_14472_length_5122_cov_3.390172, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000052 DAOSEW010000000
VERSION     DAOSEW010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 5122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5122
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14472_length_5122_cov_3.390172"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PLJ02_02445"
     CDS             <1..205
                     /locus_tag="PLJ02_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HOI05307.1"
                     /translation="NFQVAWDERGNIGKRYLAQDEIGTPFCITIDFTTLEDDTVTIRQ
                     RDSTKQERIKISELDNYLLAVLA"
     gene            210..1016
                     /locus_tag="PLJ02_02450"
     CDS             210..1016
                     /locus_tag="PLJ02_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05308.1"
                     /translation="MTKKTKIIIAVSVLVALLIAGGIWFSSRGNRDQDNNEQPVTKQK
                     ITPKTNLIPVSERPFMQLEPTADGHYVVINVIEVKKPADSLNYEMEYQTGSMLQGFQG
                     FLKLDQLPASDKKLFGSQSAGGAITYHEDIKGGSLLAEFIGPEAYAVKSSWRYFTNSD
                     RQSAFSSQDTKFTIANDSLARYSYLIIYNSPGYPAEVEGEVVSDIYTVSAETSLKTIS
                     SPFTVTFTTKEEQAQIMGYDGEAWQSLESQYENGALTGSAPFMDAYLLVK"
     gene            1165..1593
                     /gene="mraZ"
                     /locus_tag="PLJ02_02455"
     CDS             1165..1593
                     /gene="mraZ"
                     /locus_tag="PLJ02_02455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOI05309.1"
                     /translation="MLIGKFYHNLEGNGRISLPKKFRETSKSWVITRGLDGCLFLLPK
                     ETFQSELNKINEVGFSKKANRDLVRLMTNEATELVVDSNGRINLPDYLIAYAQLKKAV
                     VIVGSLNRIEIWDLERYHHYLDQLESKAETIAEQAYVAEK"
     gene            1577..2497
                     /gene="rsmH"
                     /locus_tag="PLJ02_02460"
     CDS             1577..2497
                     /gene="rsmH"
                     /locus_tag="PLJ02_02460"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOI05310.1"
                     /translation="MLQKNNHHQPVLLEESLKYLAVKEGQHYLDATFGRGGHTQAILD
                     RGGLVIALDFDQEAIEAGQENFKQAIADQQLILLKENFKNLQAVIDNLSKTQAINLAG
                     ILFDFGTSSEQLTSRERGFSFSAQENDILDMRMDQDLGVTAGELLIVLSEKQLTKVLA
                     EYGGERDAHRIAKAVNKIKKENPNSLKYSRTLLAIIEKVKGSRSGKLHPATKTFQALR
                     IAVNDELNNLELALPAAFNVLQNSTALDKRLVCIAFHEGEDALVKRFFRQQSNQGLAQ
                     LLTKKPITASQAELSTNPRARSAKLRAIAI"
     gene            2512..2790
                     /locus_tag="PLJ02_02465"
     CDS             2512..2790
                     /locus_tag="PLJ02_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05311.1"
                     /translation="MNKKIRNYYRIVFLLMMIQLISTVANLSKNIAYGQQIEVLKEKQ
                     ELLISKESQLNQLIAEEISIRQLAEYPGFEDTNNLIVVTAKDNALALR"
     gene            2798..4558
                     /locus_tag="PLJ02_02470"
     CDS             2798..4558
                     /locus_tag="PLJ02_02470"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOI05312.1"
                     /translation="MTENFSVSRKRLFLAFVAIVFVVIIGRLFYWQVIKGQELKSLSE
                     QQAIRSQTQEGLRGQIYTSDGYLLVGNQEVYDLKLDRQHFSGDVEKLSQQLAAIIAQE
                     NWQFQDASDSASKNLIQQQIESNLISKFSSQASWPQLASQLSAKAKDEILSLGNPYLA
                     FDASFTRFYPEASMAAHLTGFVGRDENGNEIGRYGVEGALEDELKPRKEHGWFYTDAL
                     GTLLAGSNYQLKNLNGRDLTLTIRRDVQHIAETNLAWGIEHYGAERGEIIILDSKTGQ
                     ILALATWPNYQQWQYFKYPGEILKNPSLTRLYEPGSTFKVLTVASAIDAGVITPNTVC
                     TKCAGPRQFGTYTIRTWNDEYHPNITMTEALEKSDNIAMIFVAELLGEEKFTAYLKKF
                     GIGQNLAIDLQDDSSTYFPQKIGPVELATISFGQGISTNSLQLLRAVNVIANKGVLLE
                     PKIIKKVYDHQLQAEIEVDREIGKRVISADTAAQVTEMMVSAAEHGEAQWIASKRYKV
                     AAKTGTSQVPDPAGGYKSDETIASFIGFAPANDPQFTMLVKLENPQSSPWAAETAAPL
                     WYKTADKLLILFNITPELDN"
     gene            complement(4662..>5122)
                     /locus_tag="PLJ02_02475"
     CDS             complement(4662..>5122)
                     /locus_tag="PLJ02_02475"
                     /inference="COORDINATES: protein motif:HMM:NF036800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05313.1"
                     /translation="LAKRIGKSASYVSNSMRLLDLPDAISDGLLSQQITEGHARSLVG
                     LKEPSLMIECYKQILKENASVRRTEELVRRMKANLEKTSKRTDDGRGRPLAQDEEQVA
                     KWENKLQDYFKSSSTLKVVRSRKQTKITITLKGSPEQTQTDLEKLMNITK"
BASE COUNT         1582 a          995 c         1070 g         1475 t
ORIGIN      
        1 aaactttcag gtggcctggg atgagcgggg caatatcggt aagcgttatt tggctcaaga
       61 tgaaattggc accccgtttt gcatcaccat tgattttacc actttagaag acgataccgt
      121 gaccattcgt caacgggatt ctaccaagca agagcgcatc aaaatcagtg agcttgataa
      181 ttatttacta gctgtgttag cctaaaacta tgactaaaaa aactaaaatc attattgctg
      241 tgagtgtatt agtggcgttg ctaatcgccg gcggaatatg gtttagttcg cgcggtaatc
      301 gagatcaaga taataacgag caaccagtga ctaaacaaaa aattaccccc aaaactaatc
      361 ttattccggt gagtgaacga ccatttatgc aactcgaacc aacagctgat ggacattatg
      421 tggtgatcaa tgtcatcgag gtcaagaagc cagcagacag tctcaattat gaaatggaat
      481 accaaacggg ttcgatgctt caaggttttc agggattttt aaagcttgat caattaccag
      541 cgagtgacaa aaaacttttt ggttcacagt cggctggggg agccatcacc tatcatgaag
      601 atatcaaagg tggtagttta ctagctgagt ttatcggacc agaagcctat gcggtcaaaa
      661 gtagttggcg ttattttacc aatagtgacc gccaaagtgc ctttagttcc caagacacta
      721 aatttaccat tgccaacgac agcttagctc gttattccta tctaattatt tataattcgc
      781 caggatatcc cgctgaagtt gagggcgaag tagttagtga catttataca gtcagtgcag
      841 aaactagcct taaaacgatt agttcaccat tcacggtcac ctttaccacc aaagaagaac
      901 aggcccaaat tatgggctat gatggagagg cgtggcagag ccttgagagc caatatgaaa
      961 acggtgcttt aactggtagc gctcctttca tggacgccta tttgctggtt aagtaactaa
     1021 atttttcatt caacaagtcg atccacaggt ttataactat tttcctaggt aggtaactat
     1081 ttttaggggc atttaactat ttacaatagt aatttttttc tatatagtta aatatggtag
     1141 gaattggtaa ataggctcat aataatgctc atcggcaagt tttatcataa cttagagggt
     1201 aatggtcgaa tatctttacc caaaaaattt cgagaaacta gcaagtcctg ggtgattact
     1261 cgaggtttag atggttgcct ttttctgtta ccgaaagaaa cttttcagag tgaattgaat
     1321 aagatcaatg aagtcggttt tagtaaaaaa gctaaccgtg atctagtcag attaatgact
     1381 aacgaagcca ctgaattagt agttgatagt aatggtcgaa taaatctgcc cgattatttg
     1441 attgcttacg ctcaactcaa aaaagcagtg gtcattgttg gcagtctcaa tcggattgaa
     1501 atctgggatc tagagcgcta ccaccactat ctcgatcaat tagaaagtaa agctgaaaca
     1561 attgctgaac aagcttatgt tgcagaaaaa taaccaccac cagccagtgc tacttgagga
     1621 gagtctgaaa tatctggctg ttaaagaggg tcagcattac ttagatgcca cctttggtcg
     1681 aggcgggcac actcaagcta ttttagatcg tggcggattg gtaattgctt tagattttga
     1741 tcaagaagcc attgaggctg gtcaagaaaa ttttaaacaa gccatcgctg atcagcaact
     1801 gattttactc aaagaaaatt ttaaaaacct acaagcagta attgataacc tgagtaaaac
     1861 tcaggcgatc aatttggccg gtatcctgtt cgattttggc acttccagcg aacaattaac
     1921 ttccagggag cggggtttta gttttagcgc ccaggagaac gacatcttag atatgcggat
     1981 ggatcaagat ttaggcgtga ctgctgggga actgctaatt gtcctaagtg aaaagcaatt
     2041 aactaaagtc ctagccgaat atggtggaga aagagatgcc caccgtatcg ccaaagccgt
     2101 gaacaaaatc aaaaaagaaa atcctaatag tctcaaatac agtcgcacct tattggcaat
     2161 tattgaaaaa gtcaagggta gcagatctgg caaattacat ccagcgacta aaactttcca
     2221 agccctacgc atcgctgtta acgatgaatt aaacaatcta gagctcgcct tacccgccgc
     2281 ttttaatgtt ttacaaaaca gcaccgctct cgataaaaga ttggtttgca ttgcttttca
     2341 tgagggtgaa gatgctctag tcaaaagatt ttttagacaa caaagcaatc aaggcttagc
     2401 ccaattatta accaaaaaac caatcacagc tagtcaagcc gaattatcaa ctaacccaag
     2461 agcgaggagc gccaaattaa gagccatagc catttaatag aaggagttca tatgaataaa
     2521 aaaatcagaa attactacag aatcgtcttt ttattaatga tgattcaatt aattagtact
     2581 gttgccaacc tgagtaaaaa tattgcctat ggtcaacaaa ttgaagtgct caaagaaaaa
     2641 caagaattat taatttctaa ggaaagtcaa ttaaaccaac tcatcgctga agaaatatcc
     2701 attcgacaat tagctgaata tccaggtttt gaggatacta acaatttaat agtagtcacg
     2761 gctaaagaca atgctttagc gctacgctag attaagcatg accgaaaact ttagtgtttc
     2821 tagaaaaagg ctttttttgg cttttgtcgc catagttttt gtggtgatta ttggccgact
     2881 tttttattgg caagtcatta aaggccaaga attaaaaagt cttagcgaac agcaagccat
     2941 tcgtagccag actcaagaag gtcttcgtgg ccaaatttac actagtgatg gttatctgtt
     3001 ggtaggcaat caagaagttt atgatctcaa attagatcgg caacatttct ctggagacgt
     3061 ggaaaaactc agtcaacaat tagcagcgat tattgctcaa gaaaactggc aattccaaga
     3121 cgccagcgat agtgccagca aaaatttaat tcagcaacaa attgaaagta atttaattag
     3181 taaattttct agtcaggcga gctggcctca actagccagc caattaagtg ctaaggctaa
     3241 agatgaaatt ttgtctctag gcaatcctta tctagctttc gatgctagct ttacccgttt
     3301 ctatcctgaa gcttccatgg cggcccatct cactggtttt gtgggtcgag atgagaatgg
     3361 taatgaaatt ggtcgttatg gggtagaggg tgccttagag gacgaattga aaccaagaaa
     3421 agagcatggc tggttctata ccgatgcgct gggcacttta ttagctgggt ctaattatca
     3481 attaaaaaac ctaaatggtc gtgatttaac tttaactatc cgccgtgatg tgcaacatat
     3541 cgctgaaacg aatttagctt ggggaattga acactacgga gcggaacggg gggaaattat
     3601 tatcttagat agtaaaacgg gccagatttt agctctagcg acgtggccaa attatcaaca
     3661 gtggcaatat ttcaaatatc ctggtgaaat tttgaaaaat ccgagtttaa ccagacttta
     3721 tgagccagga tccacattta aagtattaac tgtcgccagc gcgatcgatg ctggcgtcat
     3781 tactccaaat actgtgtgta ccaagtgtgc cggcccaaga caatttggta cttacactat
     3841 taggacgtgg aatgatgaat atcaccccaa catcaccatg accgaagctt tggaaaaatc
     3901 tgacaatatc gctatgattt ttgtggccga attacttggg gaagaaaaat tcaccgccta
     3961 tttgaaaaaa tttggaattg ggcaaaattt agcaatcgat cttcaagatg atagtagcac
     4021 ttattttccc caaaaaattg gaccagtgga gttggccacc attagttttg ggcagggcat
     4081 aagcaccaat agcttgcaac tgctgcgcgc cgtgaatgtg atcgccaata aaggcgttct
     4141 actggaaccg aaaatcatta aaaaagttta tgaccaccaa ttacaagcag aaattgaagt
     4201 cgatcgtgaa attggtaagc gagtgattag tgctgacaca gccgcccaag tcacggaaat
     4261 gatggtttct gccgctgaac atggtgaagc ccaatggatt gcctccaagc gctataaggt
     4321 ggctgccaaa acaggcactt ctcaagtgcc tgatccagca ggcggttata aaagtgatga
     4381 gacaattgct agttttattg gttttgctcc ggccaatgat ccacaattta ctatgttggt
     4441 taaattagag aatccccaat caagcccttg ggctgccgaa acagcggctc ccttgtggta
     4501 taaaactgcc gacaagttgc tgattttgtt taatattacc cctgaactgg ataattaatt
     4561 gttggcgact attgcccaac ttattgagta atacttgttt gattggctat ttgccgatac
     4621 ctattttact gttttacttg gtaatactta attttatggt tttacttggt gatattcatt
     4681 aatttttcta aatcagtttg agtttgttct ggcgatcctt tgagcgtgat cgtgatcttg
     4741 gtttgcttgc gggagcgcac aactttaagc gtgctactac ttttaaaata atcttgcaat
     4801 ttattttccc acttagccac ttgttcttca tcctgggcca gaggccggcc tcgaccatcg
     4861 tcagtcctct tactagtttt ctccagatta gctttcattc gtctaactag ttcttcggtt
     4921 cttctgaccg aagcattctc tttaagaatt tgtttgtaac attcaatcat taaagatggt
     4981 tctttcaaac ccaccaatga gcgagcgtgt ccttcagtga tttgttgact caataagcca
     5041 tcgctgattg catcgggtaa atcgagtaat ctcatgctgt tactaacgta tgaagcggac
     5101 ttgccaattc ttttggctaa ct
//
LOCUS       DAOSEW010000053         4984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_15013_length_4984_cov_4.012984, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000053 DAOSEW010000000
VERSION     DAOSEW010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 4984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4984
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15013_length_4984_cov_4.012984"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /gene="prs"
                     /locus_tag="PLJ02_02480"
     CDS             complement(<1..598)
                     /gene="prs"
                     /locus_tag="PLJ02_02480"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOI05314.1"
                     /translation="MYIIAGSSNPPLAKKIANKLGTTTKSKKFQLIPIEISKFANDEK
                     RIWINDNQLLKNQEVCLIQSFNAPVDEHIIETLLIIDALERLGAKSVTLIIPWLGYSL
                     QDKVFRPGEAIAAKVVADVISNHFVERVFLLDLHNSSIPGFFSVPTYHLFADEIFINY
                     LKKAIDFNQALIASPDFGGIKRARIFAEKVGLDMVNIDK"
     gene            complement(633..878)
                     /locus_tag="PLJ02_02485"
     CDS             complement(633..878)
                     /locus_tag="PLJ02_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05315.1"
                     /translation="MTSKENGQFSDGCQASAKERRKWCEVGDPNYCDCVEKKKCTYAD
                     FKVNLGKPNLRGGIETSDDSAKWRHWKKVYGIPHGDD"
     gene            complement(946..1356)
                     /locus_tag="PLJ02_02490"
     CDS             complement(946..1356)
                     /locus_tag="PLJ02_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05316.1"
                     /translation="MTKETPLKNSEVLNESSPVMIVIEDSSFYGKIHQRYLKDLGVET
                     VILHGIGNFDERIAEIKKTENIAGVITDGLHGNWVSVYQASINNDIKNVWLITGNQDI
                     MKESRNYPYVTAMNKASLHQNPEIYKLMVGHLAK"
     gene            complement(1420..1493)
                     /locus_tag="PLJ02_02495"
     tRNA            complement(1420..1493)
                     /locus_tag="PLJ02_02495"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1457..1459),aa:Lys,seq:ctt)
     gene            complement(1523..1595)
                     /locus_tag="PLJ02_02500"
     tRNA            complement(1523..1595)
                     /locus_tag="PLJ02_02500"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1559..1561),aa:Arg,seq:tcg)
     gene            1687..1771
                     /locus_tag="PLJ02_02505"
     tRNA            1687..1771
                     /locus_tag="PLJ02_02505"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1720..1722,aa:Leu,seq:cag)
     gene            1825..1908
                     /locus_tag="PLJ02_02510"
     tRNA            1825..1908
                     /locus_tag="PLJ02_02510"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1858..1860,aa:Leu,seq:gag)
     gene            1916..1989
                     /locus_tag="PLJ02_02515"
     tRNA            1916..1989
                     /locus_tag="PLJ02_02515"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1950..1952,aa:Val,seq:tac)
     gene            2054..2126
                     /locus_tag="PLJ02_02520"
     tRNA            2054..2126
                     /locus_tag="PLJ02_02520"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2088..2090,aa:Phe,seq:gaa)
     gene            2139..2214
                     /locus_tag="PLJ02_02525"
     tRNA            2139..2214
                     /locus_tag="PLJ02_02525"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2172..2174,aa:Gly,seq:gcc)
     gene            complement(2238..3263)
                     /locus_tag="PLJ02_02530"
     CDS             complement(2238..3263)
                     /locus_tag="PLJ02_02530"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOI05317.1"
                     /translation="MEKPQKDELVVKISPSIVIFIFLFLASIWFVGKIKNVLFITFIA
                     YIISVGLNKPISSIQDKFHWKRSTSSVLVYFLLAIIAGIFLALIVPPLVGEFSTMLSS
                     FKLPAEIDIVPNSVELNLQDISQFTNQWKDSVSTAISVVGSTFSGAVTVVMTLVISLY
                     ISIEKGQVIRDFSWLTQDKNKIKQFEEITQETNLLLGNWIRGEFIAMTIVGVLVFIGT
                     ALIGLPYPLPLALLAAIMEIIPNIGPTVSAIPAIIIALVTLGWPGAIITAVLYIVIQQ
                     LENNLIVPRVMQKSIHVSGLTVILGILICGTIFGVVGALLAIPSIIVLKTIFETWIKY
                     KPKGGLI"
     gene            complement(3274..4254)
                     /locus_tag="PLJ02_02535"
     CDS             complement(3274..4254)
                     /locus_tag="PLJ02_02535"
                     /inference="COORDINATES: protein motif:HMM:NF013845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05318.1"
                     /translation="MPNKPIKQQLIAITGATATGKSTLALKLAEEIIKKDLAKHVFLL
                     SVDSRQVYQGLEILTAADIPHGWQIIEEKPYRHFLHPQLAISLHGVSIIPIWQTWSPA
                     HFAKLYHNLKAKMTEDDVLILVGGTGFYHKQIIQPADTLGISPNPTLRQQLNKKSLGF
                     LQEQLRQFDREKFEAMNSSDSQNPRRLIRAIEVAQANQAGNKKTTNNDRQENHILNLH
                     LSLNTQKQQEQIERRVAERFKDAVHEVRAVLHQFKLSPELPAASSLGFQEILQYLNHQ
                     LDAQQAILAWQRAEIQFSKRQTTWWKKVPGLINLEADDPQLLTKAFQLVL"
     gene            complement(4255..>4984)
                     /locus_tag="PLJ02_02540"
     CDS             complement(4255..>4984)
                     /locus_tag="PLJ02_02540"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOI05319.1"
                     /translation="YARGREKSRPFSDIMEEVNGLVKEGYQEITLIGQNVNSWGLEKI
                     GVANRKMELHSALKRESLPSNQSQYLTPSGTPPFVELIRAISQFDEIKTLRFMSSNPW
                     DFHDQLIEEIANNHKLDRFIHLPVQSGSDSVLYRMNRGYTREDYLNIIKKLRQAAPDV
                     VLGTDIIVGFPGETEEEFADTVDLAKKVNWHLAFVNIYSPRPGTSANQLYRDDVPYAI
                     KKRRWQILDELINKKNLKQRPKVV"
BASE COUNT         1513 a          955 c          931 g         1585 t
ORIGIN      
        1 ttttatcgat attaaccata tctaagccaa ccttctccgc aaagattctg gctcttttga
       61 ttccgccaaa atctgggctg gcaatgagag cttggttaaa atcgattgcc ttttttagat
      121 aattaataaa aatttcatcc gcaaacaaat gataagtggg aaccgagaaa aaaccaggaa
      181 tactggaatt gtgtaaatct aatagaaaaa ctctttcgac aaaatggttg gagataacat
      241 cagctactac tttggcagca atggcctctc ctggccgaaa aactttatct tgaagtgaat
      301 aacccagcca aggaataatt agggtcacac tttttgctcc taggcgctcg agtgcatcaa
      361 taattaataa ggtttcaatg atatgttcat cgaccggagc attaaaactt tgaattaaac
      421 agacttcttg gtttttcaat aattgattat cattaatcca aattcttttt tcatcattag
      481 caaatttgga aatttcgatt ggaatcaatt gaaatttttt agattttgtc gttgtaccaa
      541 gcttatttgc aatttttttt gcaagtggag ggttggagct accagcaatg atatacattt
      601 tttatttaat ccaattaaca attaaaacga ttttaatcat ctccgtgagg gataccataa
      661 acttttttcc agtgtcgcca tttagctgaa tcgtctgaag tttctatacc acctctaaga
      721 ttgggtttac caagattaac tttaaaatct gcgtaagtac actttttttt ctctacacaa
      781 tcacaatagt ttggatcacc aacctcgcac cactttcttc tttctttggc acttgcttgg
      841 caaccgtcag aaaattggcc gttttctttt gatgtcatat ttgctttcta ttagactgac
      901 tattatatca agtttaaatc cataaataaa gaaaaaagtt aaaaactatt ttgctagatg
      961 accgaccatc agtttatata tttccggatt ttgatgaaga gaagccttgt tcatggctgt
     1021 aacatacgga tagtttctac tctctttcat gatatcctgg ttgccagtga tcaaccaaac
     1081 atttttaatg tcattattaa ttgaagcctg atagacgctt acccaattgc catgaaggcc
     1141 atctgtaatg acgccagcga tattttcggt ttttttaatc tcggcaattc tttcatcgaa
     1201 attacctatt ccatgtagaa tgacagtttc aacgcctaaa tctttcaaat atctttgatg
     1261 aatttttcca taaaagcttg aatcttcaat cacaatcatc actggtgaag attcattcaa
     1321 gacttctgag ttttttaatg gggtttcttt ggtcatttga ttaattttca gatgattggc
     1381 agatccaatt atcaaaaaaa gtaaataaat aaatcaaagt gagtcgggag ggactcgaac
     1441 cctcgacact ctgcttaaga ggcagatgct ctaaccaact gagctaccga cccgatttta
     1501 attatctaat tataacaaaa agtgccccca gggggattcg aacctccatc actggttccg
     1561 aagaccatta ctctaatcca ttgagctatg ggggctaagt ttttaatttc ttcaatcctt
     1621 taatccctcg gtctccagga tattataatc caaaagcatc gaaaaaatag tataatctct
     1681 ctataagccg agatggcgga atggtagacg cgcacgtttc aggggcgtgt gagctttgct
     1741 catggaggtt caagtcctct tctcggcacc agagttgtca tttataataa gcagttactg
     1801 acagtaaagg ctctttaatt ttaagcccgg gtggcgaaat ggtagacgcg cagtcttgag
     1861 gtggctgtga agcaattctt ggaggttcaa gtcctctccc gggcaccaat agcaggggta
     1921 attagctcag ttggttagag cgcatcgttt acatcgatga ggccataggt tcgagtccta
     1981 tattacccac aaattaccca cgacgacttt gcttgtagca aaaaatcgtt tacaatacag
     2041 aagttttaac aacgccaagc tagccaagtt ggtcacggca catgtctgaa aaacatggga
     2101 tcttggttcg attccaaggc ttggcacggt ttaaaaaagc gagagtagtt cagtggctag
     2161 aatgtctcct tgccaaggag aaggtcgcgg gttcgaatcc cgtctctcgc tccataaagt
     2221 aaatgcaaaa ttaagcacta gatcaaaccg ccttttggtt tatatttaat ccaagtttca
     2281 aaaatggttt ttagcacaat aatgctggga atagccaaca acgccccaac tactccgaaa
     2341 atcgtcccac agatcaaaat acctaaaata acggttaaac cgctaacgtg aatacttttt
     2401 tgcataactc ttggcacgat taagttattt tctagttgtt gaataacgat gtatagtact
     2461 gcagtaataa tggctcctgg ccaacctaaa gttactaaag caatgataat tgctgggatg
     2521 gcagaaacgg tgggaccgat attgggaata atctccataa ttgctgctag aagtgccaga
     2581 ggtaaaggat agggtaagcc gattaaagca gtgccaataa acactaacac acccactatc
     2641 gtcatcgcaa taaactctcc tctaatccaa ttacctaata gaagatttgt ttcttgagtt
     2701 atttcctcaa attgcttaat tttgtttttg tcttgagtaa gccaagaaaa atctctaatg
     2761 acttgccctt tttctataga aatatataaa gaaataacca aggtcataac tacagtaacg
     2821 gctccagaaa aagttgaacc aactacgcta atggcggtag acaccgaatc tttccattga
     2881 ttagtaaatt gactgatatc ttgtaaattc aactcaaccg agttgggcac aatatctatt
     2941 tctgcaggta atttaaaaga acttaacatg gtagaaaatt ctccaaccag aggtggtacg
     3001 ataagggcta aaaagatgcc agcgatgatg gcaagcaaaa aataaaccaa aacactacta
     3061 gttgatcttt tccaatgaaa cttatcttgg atactgctga ttggcttatt taagccaaca
     3121 ctaataatat aagcgataaa agtaataaat aagacatttt taatttttcc aacaaaccaa
     3181 atgctagcaa gaaaaagaaa aataaaaatt acaattgacg gtgaaatttt aactaccaat
     3241 tcatcttttt gtggtttttc catagtacta atactatagc acaagttgaa aagctttggt
     3301 caaaagttga ggatcatcag cttccaaatt aattaagcca ggtacttttt tccaccaggt
     3361 tgtttgtcgt ttgctaaatt gaatttctgc cctctgccaa gccagtatgg cttgttgagc
     3421 atctaattga tgatttaggt attgcaagat ttcttgaaaa ccaagactac tggccgctgg
     3481 caattctggg cttaatttaa attgatgaag aacggctctt acttcgtgga cagcgtcttt
     3541 gaaacgttcg gccactcgcc tttcaatttg ttcttgctgt ttttgggtat ttagactgag
     3601 gtgaagattg agaatatgat tttcctggcg atcattgttg gttgttttct tatttcctgc
     3661 ttggtttgct tgcgctactt cgattgccct gattaagcgt cgaggatttt gtgaatcaga
     3721 cgagttcatg gcttcaaatt tttctcggtc aaattgcctc aattgttcct gcaaaaaacc
     3781 tagggacttt ttgtttaatt gttgacgtag agttggattc ggggaaattc ccaaagtatc
     3841 cgccggctga ataatttgtt tgtgataaaa gcctgtgcca ccaaccaata ttagaacgtc
     3901 gtcttcggtc atttttgctt tgagattatg ataaagtttg gcaaaatgtg ctggtgacca
     3961 agtttgccaa ataggaataa tactcactcc atggagactg atagctaatt gtgggtgaag
     4021 gaaatgacga tagggttttt cttcaatgat ttgccaacca tgaggaatgt cagccgctgt
     4081 taaaatttct aaaccttgat aaacctgtcg cgaatcaaca gaaagtaaaa agacgtgctt
     4141 ggcaaggtct tttttgataa tttcctcggc cagttttaaa gctaatgttg atttcccagt
     4201 tgcggtagca ccggtaattg caatcagctg ttgtttaatt ggtttgtttg gcatctacac
     4261 tacttttggt cgttgcttaa gatttttttt attaatcagt tcatcaagaa tttgccaacg
     4321 gcgttttttt attgcgtagg gaacatcatc tcgataaagt tgattagcac tcgtaccggg
     4381 acgaggtgaa taaatattga caaaagccaa atgccaatta acttttttag ccagatcaac
     4441 agtatctgca aattcttctt cagtttctcc agggaacccc acaataatat cggtgcctaa
     4501 aacaacatcg ggagcggctt gacgcaattt tttgataata tttaaataat cttcacgagt
     4561 ataaccacga ttcatgcgat ataagacact atcactgccc gattgtactg gtaaatgaat
     4621 aaaacgatct aatttatgat tgttagctat ttcttcgatt aactgatcgt gaaaatccca
     4681 gggattacta ctcataaaac gcaaggtttt gatttcgtca aattgagaga tggctctaat
     4741 aagttcaaca aaaggtggag tccccgaggg ggtcagatat tgagattggt tactgggtaa
     4801 actttctctt tttaaggctg agtgtagttc cattttacga ttagctaccc caattttttc
     4861 tagtccccaa gaattaacat tttggccaat taaagtaatt tcttgataac cttcttttac
     4921 taagccatta acttcttcca tgatgtcgct gaatggccgc gacttctctc taccccgagc
     4981 ataa
//
LOCUS       DAOSEW010000054         4973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_15059_length_4973_cov_3.615697, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000054 DAOSEW010000000
VERSION     DAOSEW010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15059_length_4973_cov_3.615697"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PLJ02_02545"
     CDS             <1..292
                     /locus_tag="PLJ02_02545"
                     /inference="COORDINATES: protein motif:HMM:NF013085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HOI05320.1"
                     /translation="VGYIASYTHATGQIASLLELLCETDFVAANEEFRDLAKNLAMQV
                     AAMNPQSKADFLAQDYIRGEQNVELTIKALSGKIGEKIVLGRFERFAVGEQE"
     gene            360..1331
                     /locus_tag="PLJ02_02550"
     CDS             360..1331
                     /locus_tag="PLJ02_02550"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HOI05321.1"
                     /translation="MRDYCPSRREQKKLKAKKVKARKVRRSKKKSAVVNWQKLSLFFL
                     SLFLLLLLGLLFYYLLKNQLAKRHQLSRNQHLLVVDSKLPVAWVVFAPLEQKIKVFDF
                     SQLSVANWPREEFAREMDANEEILFYSLLFNTFIDQVIAYPQQNLSKENQAQFSDFLM
                     TELRSREEQNLLFYLEQSQVSWEWFNDTEAKTREDKENLLSSFLARNTEASYGKLFEC
                     PVAVINSSSVTGLASAFAQLLEKDGFSVIKRDSGQDILAETSLLIDPEITACEALLNR
                     FQQLMPVKVINADHELAQQYRAGAVIFLAEDLAKLRIGTFDFLHDSL"
     gene            complement(1290..2048)
                     /locus_tag="PLJ02_02555"
     CDS             complement(1290..2048)
                     /locus_tag="PLJ02_02555"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05322.1"
                     /translation="MTMSNSSLLRAKAIQAVKDSDWENAVLINQEILERSPKNLEAMN
                     RLGLAYLKLKKEKEAKKVFNNVLKLDRSNIIANKHMKKLKNKEKTPDVIFNHNADFIE
                     EPGKSKIISLHRLTGSSQLAKLKVGQECYLQIKNRYISVTDDQQKHIGTLPEDISFRL
                     SKLISDGNQYSCVIYKIDDNQCCVQLKETYRSAKNQQLQSFPSKKINNQFNLDEDIIL
                     ETDIPLGIVSTDEDDDEDDDGLETVMKKIESSDS"
     gene            2155..2712
                     /locus_tag="PLJ02_02560"
     CDS             2155..2712
                     /locus_tag="PLJ02_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOI05323.1"
                     /translation="MSGHSKWNNIKNKKNAQDQIKGKIFSQFSRQIRAAVKEGKSGDP
                     SLNANLRVVLEKARSVNMPKDKIQRAIDVALGKTGADQVRENVYEAFAPGGVALMIVA
                     LTNNNNRTASELRTLLTKHGGSLAGPGSAAYMFERDREGGFTAKIKIKLDEQQTAQVL
                     ELVDLLREHEDIEDVFVATDLPEED"
     gene            2717..3484
                     /locus_tag="PLJ02_02565"
     CDS             2717..3484
                     /locus_tag="PLJ02_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05324.1"
                     /translation="MRRREKFIIVSVLLSLGLLATQYVPIDWRYAAIGIFALISYLMS
                     AWALSDDLQIYEWLTILPLPVLYASSVALFYFLLPEVLLSRLAILAVFGVGMYALFLT
                     ANIFSVAKGRTIQLLYAARAIALFFTMIISLLLTNTIFSLRLPFYLNALLVAISHFML
                     VLMSLWSVQLEPRISKQILNYTLLFVLILVEFTIAFSFLPMEIWYISLFIMSLVYVGL
                     SLFQDLLQGRLFKNTATEYTLVSIFIILAFMIVFPGK"
     gene            4644..>4973
                     /locus_tag="PLJ02_02570"
     CDS             4644..>4973
                     /locus_tag="PLJ02_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05325.1"
                     /translation="MSENAVFLAKPQLLYQKAFIEAVAELQATQHDRYYVEKSLDLTK
                     LAKKRFFQAYLKDIQEQEAGIDLPVGRVPQTIYWLMKKPGDGQVIWLGRVAIRHQLND
                     NLRKIGGH"
BASE COUNT         1488 a          842 c         1034 g         1609 t
ORIGIN      
        1 tgttggttac attgccagct atactcatgc tacaggccaa attgctagtt tgctggaatt
       61 actttgcgag actgattttg ttgctgctaa cgaagaattt cgtgatttag caaagaattt
      121 agctatgcaa gtggctgcca tgaatccgca aagcaaagca gattttttag ctcaggatta
      181 tattcgaggt gaacaaaatg tagaattgac catcaaggct ttatctggaa aaatcggtga
      241 aaaaatagtt ttgggtcgtt ttgagcgttt tgcggttggt gagcaagaat aaaaattgct
      301 aaacaccgtc atggtcagca agaataaaaa ttgctaaact ataagttgaa aattgtttta
      361 tgcgtgatta ctgcccatct cgtcgagaac aaaaaaagct taaggctaaa aaagtcaaag
      421 ctcggaaagt tcggcgatct aaaaaaaagt ctgccgtagt gaattggcaa aaactgtcat
      481 tattttttct ctccttattt ttattacttt tactcggctt gctgttttac tatttattga
      541 aaaatcagct agccaaacgc catcaactca gtcgcaatca acacctttta gtagtagata
      601 gcaaattacc agtagcctgg gtagtttttg ccccgctaga gcaaaaaatt aaggttttcg
      661 attttagtca gttgagtgtg gccaattggc ctagagaaga gtttgctaga gaaatggatg
      721 ccaatgaaga aattttattt tatagcctct tgtttaacac ttttattgat caggtcattg
      781 cttatccgca acaaaactta tccaaagaaa accaagctca atttagcgat tttttaatga
      841 ctgaattgcg gagtagagaa gagcaaaatt tgcttttcta tctagagcaa agccaggtca
      901 gttgggaatg gtttaacgac actgaggcaa aaacacggga ggataaggaa aatttattga
      961 gtagtttttt ggcaagaaat actgaggcca gttatggtaa gttatttgaa tgtccagtag
     1021 cggtcattaa tagtagcagt gttactggat tggctagtgc ttttgcccaa cttttagaaa
     1081 aagatggctt ttcggtaatt aaaagagata gcggacaaga tattttggca gagacttctt
     1141 tactaattga tccagagatc acggcttgcg aagccttatt gaatcgtttt caacaactta
     1201 tgccggtcaa ggtcattaat gccgatcacg aattagcgca acaatatcgg gcgggagcag
     1261 tgattttttt agccgaagat ttagccaaat taagaatcgg aactttcgat tttcttcatg
     1321 acagtctcta gaccgtcatc gtcttcatca tcatcttcat cagtgctgac gataccgaga
     1381 ggaatgtccg tttccaaaat aatatcttca tctaggttaa attgattatt aatttttttg
     1441 ctaggaaaag attgtaattg ttgatttttg gctgagcgat aagtttcttt gagttgaaca
     1501 caacattggt tatcatcaat tttataaatc acacaactat attggttgcc gtcactaatt
     1561 aatttactca agcgaaacga aatatcttca ggcagcgtgc caatgtgttt ttgctgatca
     1621 tcagtaacac taatgtaacg atttttgatt tgtaagtaac attcttggcc aacctttaac
     1681 ttggctaatt gagaagagcc agttaaacgg tgcagggaaa taattttact tttgcctggt
     1741 tcttcaatga aatcagcatt atggttaaaa attacgtctg gagttttttc tttgttcttt
     1801 aacttcttca tgtgcttgtt agcaataata tttgaacggt caagcttaag aacattatta
     1861 aaaacttttt tcgcttcttt ttcttttttc aatttaagat aggcaagacc caaccggttc
     1921 atggcctcta aattttttgg agagcgttcg aggatttctt gattaatcag gacagcgttt
     1981 tcccaatcag agtctttaac tgcctgaatg gctttggctc gcagcagaga gctgtttgac
     2041 attgtcatat ggctgataga cctttctttg ggggatacta caacccacaa aaagaattgt
     2101 caactcacaa aaagcaaatg cttggggttg cggaagcttt gctataatag caatatgtca
     2161 ggacacagta aatggaacaa tatcaagaat aagaaaaacg cccaagatca aatcaagggc
     2221 aaaattttta gccagttttc tcggcagatc cgggctgctg ttaaagaggg taagtctggt
     2281 gatccaagtc ttaatgctaa tttaagagtg gttttagaaa aagccagatc ggttaatatg
     2341 ccgaaagata agatccagcg agcgattgat gtggctttgg gtaaaactgg agctgatcag
     2401 gttcgggaaa atgtctatga agcttttgct ccgggtggag tagcgctaat gatagtagcc
     2461 ctcacgaata ataacaaccg aaccgctagt gaattaagaa ctttactaac taagcatgga
     2521 gggtctttgg cgggtccagg tagcgctgct tatatgtttg aacgtgatcg agagggcggt
     2581 tttactgcca aaattaaaat taaattagat gagcagcaga cagctcaagt gcttgagtta
     2641 gtagatttat tgagagagca tgaagatata gaagatgtgt tcgtggcaac agacttgcca
     2701 gaagaagatt aacactatgc gccgccgtga aaaatttatt attgtctcag tcttattgtc
     2761 tttaggttta ttagcgactc aatacgttcc aattgattgg cgctatgcag cgattggcat
     2821 atttgcgctc attagttatt tgatgtcggc ttgggcgctg agcgacgatt tacaaattta
     2881 tgagtggtta acgattttgc cgttaccagt actatatgct tcttcagtag cgctgtttta
     2941 ttttctttta ccagaggttt tattatctcg tttagccatt ttggctgttt ttggcgtggg
     3001 catgtatgct ttatttttaa cggctaatat tttttctgtt gctaagggaa gaaccattca
     3061 gcttttatat gctgcgcgcg ccatagcctt gttttttacc atgattattt cactactttt
     3121 aactaatacc attttttcac tacggttgcc tttttatctt aatgctttgc tggtagccat
     3181 ttctcatttt atgctagtgt taatgtcgct gtggtcagtc caattagaac cgcggatcag
     3241 taagcaaatc ttgaattaca cattgctatt tgtgttgatc ttggttgaat ttaccatagc
     3301 tttttccttc ttacccatgg agatttggta tatttcttta tttattatgt ctctggttta
     3361 tgttggctta tcattatttc aagacttgtt gcagggtaga ctatttaaaa atacagctac
     3421 cgaatatacg ttagttagta tttttatcat tttagctttt atgattgttt tccctggtaa
     3481 ataaacaatt tatattgata tatcaatata tttatagata attagagtgg ggcagagctg
     3541 gtaaaaaaaa ctggggcttg atgagttttg tggtggctgc aatataaaaa aattgcgtcc
     3601 atatttcgct taatttagca aactataata gattttttga gtaaaatttc ataaaaaatc
     3661 gccgaaaatt actagggtag tctgtagcta aatttcaaga ggagatgcgt ttgtaaaata
     3721 aaaaatgtgg caaagatggg ctaaagcaag tactattgcg cccggctata gggcataggc
     3781 gaacaaaaaa atctgttagt caggagcaaa gggtagaggc gtgaaaattg tggaaaactc
     3841 actagttata ggaccttgct agcctgggat gatatagttt gatatagacg atttaaatca
     3901 gccaacagtt gtgatgcaaa aataaattta gcctggtgca agaataaaca atcagcaaaa
     3961 tctgctctga attttctcga gaaaccaagc caaattagtg gtccggccct agggagcagc
     4021 agcactgata aaatcaagaa aaaacccgaa gaggcctttt tggagacact cagggagggg
     4081 ttgctagtgg caaattagat ctgggcagga taatgagctg agggtttagg gagtgtagtg
     4141 taatttacca attaaattta actaaaacca cagtttactc tttactactc tgcggcagag
     4201 tttgacagtg gttgcgacag gaagatggag atgctatgtc gcacaatata acttttgcga
     4261 cactgaacac catttgccac tagagcgctc tttttagcta gttaaccttc atttaccaat
     4321 taagcgctat tttctttttt aattggttta tttactttta ttttcctgtc tgccctcatt
     4381 ttttgcccca cgttaaaaat tactggtttc tggcaaattg ctgctttctg ccaaattgcc
     4441 gatttttggc tagaactccc ctaaagctat ttttttacca gatatacgcc agatgtcatg
     4501 gctgaggttt tcagggtaat taattatatt ttaaattgat atatcaatct aaaaccctca
     4561 agaagctcgt taaaagccca aaatcggctg ttttttcttt ttccgaataa tccatcatgg
     4621 aaaatcaggc tataatagcc agtatgtcag aaaatgcagt ttttttagca aaaccacaat
     4681 tactttacca aaaagcgttc attgaagctg tggccgaatt gcaagcaacc caacacgatc
     4741 gctactacgt agaaaaaagt ttggatttaa ctaaattggc gaaaaaaaga ttttttcaag
     4801 cttatttaaa agatatccaa gaacaagaag ctggtattga tttaccggtt ggccgggtgc
     4861 cacaaaccat ctactggtta atgaaaaagc ctggtgacgg tcaagtgatc tggttaggtc
     4921 gggtggcaat ccgtcatcag ctcaatgata atttgcgcaa aattggtggt cat
//
LOCUS       DAOSEW010000055         4847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_15596_length_4847_cov_4.325125, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000055 DAOSEW010000000
VERSION     DAOSEW010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15596_length_4847_cov_4.325125"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..908
                     /locus_tag="PLJ02_02575"
     CDS             120..908
                     /locus_tag="PLJ02_02575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOI05326.1"
                     /translation="MLVKNLTIVIINDRRDQLFEQALLSAAIAEEIIVFDQVSHNNWS
                     TLEKKLKKINPKLNFRLIFRKNPILDFASLRNKAIAKVHTPWLMFLDSDEILSQQNLK
                     QLPTLLENEAVSAYRLKRVDYFHQKKLRFGETGNCSIIRLARSNAIRYQRVVHEIPII
                     AGQVANSHLEIKHYPHHNLNDFLNTVNNYAELEASYRFSEKYFYPKLKIILELLIYPN
                     VKFLVNYLLKLGFADGFAGLAYAVLMSFHSLLVRVYLYEKYFLN"
     gene            886..2004
                     /locus_tag="PLJ02_02580"
     CDS             886..2004
                     /locus_tag="PLJ02_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05327.1"
                     /translation="MKNIFLISFLLLFAGGELAKIYINQYLAIYLHDFLVFFYLIFNI
                     KTLKKIILNLKKIDKFNKKIFLLLLINIIFAVAYSIGQNQFQFTSFLYLGRALAYFLF
                     VLLLKKNYGQKKVVQVFLNASLLILGLGFGQYLFLPDLTNLYYLGFDDHFYRLTSTLL
                     DPNFTALIFVFNWLYYAKKANLNKKNLWFSILFLLGLALTYSRAGYLALFLSGIYLWL
                     KSQTAERRLIALSLTAFLILLPLLPKGSGGEGVNLQRTSSITARIISAQQYIKQNEGL
                     AIIFGRGPYQPHYQLNREGLVSHARFSDNFLIFFYNSFGLVGTVLILILLTQEIKNKS
                     KKKQYWQLSLLIALLTHSLFNNNLSQAFISLLFWGFYL"
     gene            complement(2001..2765)
                     /locus_tag="PLJ02_02585"
     CDS             complement(2001..2765)
                     /locus_tag="PLJ02_02585"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05328.1"
                     /translation="MSRSSRYSSVYKNKSQAKRGSSRLIKKQEKEMRKQTVFFIFLAI
                     VLLVVFIFVIVPNLIRLFFNFFGDEQANIIEDDVPPTTPLLFAAPPEATFSAQILLKG
                     YADPNAKVVFVLNGEQVEEKEVNDEGEFSQEVQLDEGVNQLSIYAVNERGTESLQTKT
                     YNIIYDNEPPQITVTEPKPDSVIELKRNRNTLVSGETEPHAKVYLNDRLIVVDEAGQF
                     STTYYLNEGENVLRFVAIDQAGNQGELEIKVKFLNN"
     gene            complement(2807..2880)
                     /locus_tag="PLJ02_02590"
     tRNA            complement(2807..2880)
                     /locus_tag="PLJ02_02590"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2844..2846),aa:Thr,seq:cgt)
     gene            complement(2910..2980)
                     /locus_tag="PLJ02_02595"
     tRNA            complement(2910..2980)
                     /locus_tag="PLJ02_02595"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2946..2948),aa:Gly,seq:ccc)
     gene            complement(3070..3146)
                     /locus_tag="PLJ02_02600"
     tRNA            complement(3070..3146)
                     /locus_tag="PLJ02_02600"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3110..3112),aa:Asp,seq:gtc)
     gene            3251..3832
                     /locus_tag="PLJ02_02605"
     CDS             3251..3832
                     /locus_tag="PLJ02_02605"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOI05329.1"
                     /translation="MFIYIVITLVVIALYVMGVYNGLQRLKTQIKASIQEIGNQLKRQ
                     AGLIPSLENAVKGQLQHEKGIYQLLTDARKSTIKAEKTGKSADINQAIADIQKLMPQM
                     QVLVENNPELKANEAITKFMDELTDTADKLTYARRSVIDLTQSYNEKLVVFPSNVVAN
                     LFGFKEEKGLETPLSGEHLSVSQAEMKAPKVDL"
     gene            complement(3829..4539)
                     /locus_tag="PLJ02_02610"
     CDS             complement(3829..4539)
                     /locus_tag="PLJ02_02610"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOI05330.1"
                     /translation="MLSKIWRKMVERLSFFLLPFGQIAYGQNAEDIVLAKFLDERLKN
                     KQKGFYIDIGAHHPFRYSNTYYFYQRGWRGLNIDADEKAIQLFKLFRPRDINVQALVA
                     KENKLRSYYIFSDRAFNTGDPHLAQAIIKEKRARLLKKTQMRSEQLPKILHQYLPTSQ
                     KVDFLSIDVEGLDEEVLQAYDFVHFAPSYLLIEDLNFINNLSRKNSTKIAPFLKKQGY
                     VLVAQVFNTAIWQKRTVA"
     gene            4600..>4847
                     /locus_tag="PLJ02_02615"
     CDS             4600..>4847
                     /locus_tag="PLJ02_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015420070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HOI05331.1"
                     /translation="MTHYQLVAKNKRRSWLIMIAFVLFVTAVSYVIAIGFDYSLSIVG
                     LALIFSGFTSFFSYYFSDKIVLTISRAKPATRREYFDF"
BASE COUNT         1510 a          890 c          849 g         1598 t
ORIGIN      
        1 atgattgccg gcattattag tttaacccca acttatggcc tctatggtgc ttgtctaggt
       61 attttattag ctagagtgat tagcctgcca attagtttat tgtcggttaa gaaaaattaa
      121 tgttagtcaa aaatctaact atcgtcatca ttaacgatcg ccgggatcaa ctgtttgagc
      181 aagcgttact tagcgccgcg attgctgaag aaattattgt tttcgatcaa gtcagccata
      241 acaattggtc tactttagaa aaaaaattaa aaaaaattaa tcctaagctt aattttcgat
      301 tgatttttag aaaaaatccg attttggatt ttgcttccct ccgtaataaa gccattgcca
      361 aagttcatac gccgtggtta atgtttttgg atagtgatga aattctaagc cagcaaaatt
      421 taaaacaatt acccacctta ctggaaaatg aagcagttag cgcttatcga ctaaaaagag
      481 tggattattt tcaccaaaaa aaattacgct ttggtgaaac tggcaactgc tcaattattc
      541 gcctagccag atcaaatgca attaggtatc aacgggtggt tcatgaaatt cctattattg
      601 ctggccaagt agcgaatagt catttagaaa ttaagcatta tcctcatcat aatctaaatg
      661 attttttaaa cacggttaat aattacgccg agcttgaggc tagttatcgc tttagtgaaa
      721 aatattttta tcccaaacta aaaattattt tagaattact gatttacccc aatgttaaat
      781 tcctggtcaa ttatttgtta aagctaggtt ttgctgatgg ctttgctgga ctagcctacg
      841 cagtgttaat gagttttcac tcattattag tgagagttta tctgtatgaa aaatattttc
      901 ttaattagtt tcttactatt attcgcgggt ggagaactgg ccaaaatcta cattaatcaa
      961 tatttggcca tttacctaca tgatttttta gtcttttttt atttaatatt taacataaaa
     1021 acattaaaaa aaattattct taatttaaaa aaaatagata aatttaacaa aaaaatattt
     1081 ttattacttt taatcaacat tattttcgca gttgcctata gtattgggca aaaccagttt
     1141 caattcacta gttttctcta cttaggtcga gcactggctt attttttatt tgtgttgcta
     1201 ttgaaaaaaa attatggcca aaaaaaggtt gtgcaagttt tcttaaatgc ttccttgctt
     1261 atacttggct taggctttgg tcagtacctg ttcttaccag atttgactaa cctttattat
     1321 ctaggttttg atgatcattt ttatcgatta actagcactc tcctagatcc taattttacg
     1381 gccttaatct ttgtttttaa ctggctctat tatgccaaaa aggctaattt aaataagaag
     1441 aatctttggt tcagcatttt gtttttgctt ggtctggctc tcacttattc tcgcgctggc
     1501 tatttagctt tgtttctatc tggaatttac ttatggttga aaagtcaaac agccgagcgt
     1561 cgactaattg ccttgagctt aactgctttt ttaatccttc taccattgct acctaagggt
     1621 tctggtgggg aaggagtcaa cttacaacgc actagttcaa taacggctag aatcatcagc
     1681 gctcaacaat acatcaaaca aaatgaggga ttagccataa tttttgggcg ggggccttat
     1741 caaccacatt atcaacttaa tcgcgaaggg ctcgttagtc acgctcgctt cagtgataat
     1801 tttttaatct ttttttataa cagctttggc ctcgttggca ctgttttaat cttaattttg
     1861 ttaactcaag aaattaaaaa taaatcaaag aaaaaacaat attggcaact aagtttactg
     1921 atcgccttat taactcatag cctgttcaat aacaatttaa gtcaagcctt tatttcttta
     1981 ctattttggg gtttttatct ttaattgttg agaaacttta ctttaatttc aagttcacct
     2041 tgattgccgg cctgatcaat cgctacaaaa cgtaatacat tttcaccttc atttaaataa
     2101 taggtagtgg aaaattgacc tgcttcatca acgacaatta atcgatcatt taaatacact
     2161 ttagcgtgag gctcagtttc gccactgacc agcgtgttgc ggttgcgttt taattcaatt
     2221 acactatctg gctttggttc agtcactgtg atttggggtg gttcattatc ataaataatg
     2281 ttatatgttt tagtttgcag tgactcggtg cctcgttcat taacggcata aatactcaat
     2341 tgattgacac cttcatccaa ttgcacttct tgactaaatt cgccttcgtc attaacttcc
     2401 ttttcttcaa cttgttcacc atttaaaaca aaaaccactt tggcattcgg atcagcataa
     2461 cccttcaaaa gaatctgcgc actaaaagta gcctctggtg gcgcagcaaa taaaagggga
     2521 gtagtcggcg gtacgtcatc ctcaataata tttgcttgtt catcaccaaa aaaattaaaa
     2581 aataatctaa ttaaattagg tacaatgaca aaaataaaaa caactaagag gacaatggct
     2641 aaaaaaataa aaaaaacggt ttgtttgcgc atttctttct cttgtttttt gatcaagcgc
     2701 gaactacccc gtttagcttg gcttttattt ttataaacgg aagaataacg actagaacgt
     2761 gacatattag ctatttaatc ataacacaat ctaaaattgt tctaggagcc aaaggtggga
     2821 gtcgaaccca cgacctgcgg tttacgatac cgctgctcta gccaactgag ctactttggc
     2881 atcttaagtt aatttaagtt atgtgattga gcgaccgaag ggagtcgaac cctctcctca
     2941 agcttgggaa gctcgcattc taccgctgaa ctacggtcgc aatagcttta ttttagcatc
     3001 attatggata aagaaataaa tcctttttta aaacaaaaag agaccagccg gtctcttaat
     3061 tttcatgttt ggcgaccccg accgggctcg aaccggcgat cttctccgtg acagggagat
     3121 atgttaacca actacaccac agggccctgt taagtagcgc tcggattata aaaggaaatc
     3181 atgaaaactg caataaacaa gcgttaattt ataaaataag ctataataaa aaaagaaaga
     3241 aaggaatttt atgttcattt atatcgtaat aaccttagtt gttattgctc tctatgtcat
     3301 gggtgtttac aacggtttgc aacgcctaaa aactcaaatt aaagctagta tccaggagat
     3361 tggtaaccaa ttaaagcgtc aagctggttt aattcccagt ctagaaaatg cagttaaagg
     3421 tcaactccaa cacgagaaag gcatttatca gctgctaacc gatgctcgta aatcgacaat
     3481 taaagcagaa aaaactggta aaagtgccga tattaaccaa gcgattgcag atattcaaaa
     3541 attaatgcct caaatgcaag tgctggtgga aaataatcca gaattgaaag cgaatgaagc
     3601 gattactaaa tttatggatg agctaactga tacagctgat aaactaactt atgcgcgaag
     3661 aagcgtcatt gatctcaccc agagctacaa tgaaaaacta gtggtctttc cttccaatgt
     3721 cgttgctaac ctttttggct ttaaagagga aaaggggtta gaaactccgc tgagtggtga
     3781 acacctcagt gttagtcaag cagaaatgaa ggcaccaaaa gtggatcttt aagcgacagt
     3841 tcttttctgc caaatggcag tgttaaaaac ttgagccacc aaaacatagc cctgcttttt
     3901 taaaaagggg gctatttttg ttgaattttt tctggataaa ttattgataa aattcaaatc
     3961 ctcgattaat aaataacttg gggcaaaatg cacaaaatca taggcctgca aaacttcctc
     4021 atccagacct tcgacatcga tagataaaaa atcaactttt tggctggtcg gtagatattg
     4081 atgcaggatt ttgggcaatt gttccgatct catttgggtt tttttaagca accgagcccg
     4141 cttttcttta attattgcct gggccaagtg cggatcacca gtgttaaatg cacgatcgct
     4201 aaaaatataa taagaacgta gcttgttttc tttggccact agagcttgca cattaatatc
     4261 tcttggacgg aataatttaa ataattgtat ggctttttca tcagcatcaa tgtttaaacc
     4321 tcgccaaccc cgctggtaaa agtagtaagt gtttgagtag cgaaaaggat ggtgagcgcc
     4381 aatatcaata taaaaacctt tttgcttatt ttttaagcgc tcatccaaaa actttgctaa
     4441 cacaatgtct tccgcatttt gcccataggc aatttgacca aagggtagta gaaaaaaact
     4501 taagcgctcg accatttttc gccaaatttt gcttagcatc gctgctcatt atacagtgaa
     4561 ttgttaccca gttaatcaga ctaatgttat aatcaaaaca tgactcatta tcagttggta
     4621 gcgaagaata aacgtcgatc ttggctaatc atgattgcct ttgtgctctt tgtgacggct
     4681 gtcagttacg tgattgctat tggttttgat tattcattat ctatagttgg cctggcttta
     4741 attttctcag gatttaccag ttttttcagt tattattttt cagataagat tgtcttaacc
     4801 atttccagag ctaaaccggc gactcgtcga gaatatttcg attttta
//
LOCUS       DAOSEW010000056         4619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_16674_length_4619_cov_4.443909, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000056 DAOSEW010000000
VERSION     DAOSEW010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16674_length_4619_cov_4.443909"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PLJ02_02620"
     CDS             <1..383
                     /locus_tag="PLJ02_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05332.1"
                     /translation="INYPVDLSQAIFIATCNNTRRISAAVLDRMERIDMPSYSDEEKI
                     VIAKKYILPEILKEAGLKENQFKIDEAVWPKLVRPLGFDAGVRTLQRTLKGAVRKAAR
                     IIVERGFEEVVIKPDNVKIFLPSY"
     gene            404..1063
                     /locus_tag="PLJ02_02625"
     CDS             404..1063
                     /locus_tag="PLJ02_02625"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOI05333.1"
                     /translation="MSKTDLPLKEMLQQVKDLSSLQLLLEENVAQLPLAKKPQDIIHG
                     EGSQNAEIMLIGEAPGYYESIQRRPFVGRSGQLLRKTLEEINLPAEKVFISNIIKVRP
                     PDNRDPLPKEILAFKPFLDKEIELIKPKLIITLGRFSLAKFLEGVKISQIHGRLHSLR
                     WQGLNLFVLPMYHPAAALRSSQVKESFVNDFKKINKILAWIAGQEETQIFKNQLREEL
                     F"
     gene            1065..>1428
                     /locus_tag="PLJ02_02630"
     CDS             1065..>1428
                     /locus_tag="PLJ02_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOI05334.1"
                     /translation="MSKLKFIALGGMGRVTQNLYLYQYEQEILLIDCGIGFPDIYMPG
                     VDALIPDITYLLNQIEGGAKIVGMILSHGHDDHIGALPYLLPNLPDFPIYASPLTAQF
                     AHDRLLDKKVDKNIQVLGD"
     assembly_gap    1429..1438
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1478..2815
                     /locus_tag="PLJ02_02635"
     CDS             1478..2815
                     /locus_tag="PLJ02_02635"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF036802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOI05335.1"
                     /translation="MSITQNIQVLGDRQNFSLGQYFKFQFVAITHSVPDSKHILLETP
                     VGKIYHGSDFKLDKHPVDGHLTDMEFIEQIGRQGDLLMACLDCLRVERNEWTPSESTV
                     GPALLEEMKGVNGKILVTLMSSHLHRIQQTVNACQELGRKVVFVGRSVEQNVQTAIQS
                     KSLNIPKGLMVDKKKIKEIPDEKLCIIIAGSQGQEGSSLVRAVYGDHQIVQISNRDKV
                     IFSADVIPGNEIPYYSAVDQLAADEVDVVYPEIRPSIHRSGHASRVEQELMLKLLRPK
                     FVMPIGGADRHRALFKKTVAKPLSYQDWQILLPKSGEVLAIDQQRVSVLQQISLSAQT
                     VDGLGIGDVGPIVLSDRLNLSKAGIIVLLLPRFKKKIDFSRMQVISRGFVFMKEADEV
                     VNYIKKETVQIVQDLGKNVTDEELKRRLEKRLARRLFKVIRREPIILPVIWDI"
     gene            2903..>4619
                     /locus_tag="PLJ02_02640"
     CDS             2903..>4619
                     /locus_tag="PLJ02_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOI05336.1"
                     /translation="MAKRKSRRKKRKAIRMKMRADTTYSIIAVLLIMFGSLVLVSFTG
                     QGQWLITLNNFLANKLGLSMLFLPFVFIASGLVMIRSKHAWSKPTILLGVIVMMIGAM
                     GVSRSGEIGINIFNNLALLISAVGSYIVYGALTIIGFLILSQSSLTDLVNGISKIISK
                     NHQKKSAKKDVKTLDKDITDQEIKKSRHFFIPKLAFGKKDLKRGSDFQKEIELDQTSS
                     TAVLTEKKVIDNKLPSNSLDGEEALNASSTPMIWDYPPLSLLTVEKGAKADRGDVKAN
                     AQIIENTLDSFGINAKVVEYNPGPAVTQYCLEISQGTRLAKITALSTDLALALAAPTG
                     QVRIEAPIPGRNLVGVEVPNHSAEIVPLKTMLSSPILKKHPSKLAVSLGVDVAGKPVV
                     ADISEMPHLLIAGATGSGKSVCVNAFLCSILFRATPEEVKLILVDPKRVELGKYHDIP
                     HLLTPVIHDPDKVISALKWAVELMEKRYKLLNEVGVRSIHDYNELAGLATMPNIVIVI
                     DEMADIMLFAPKEVEDNVTRIAQMARAVGIHLVLATQRPSVDVITGLIKANMPTRIAF
                     NVSSVT"
BASE COUNT         1424 a          761 c          963 g         1461 t
ORIGIN      
        1 acattaatta tccggtagat ttaagtcaag cgatttttat tgccacttgt aacaacaccc
       61 gtcgtatttc agccgctgtt ttagaccgga tggaacgaat tgatatgccg tcctacagtg
      121 atgaagagaa aattgtgatt gccaaaaagt atattttgcc tgaaattctc aaagaagctg
      181 gtttgaaaga aaaccaattt aagattgatg aagcggtttg gcctaagtta gttagacctt
      241 tgggttttga tgctggagta cgtacgttac aacgcacgct taaaggtgcg gtaagaaaag
      301 ccgctagaat tattgtggaa cggggttttg aagaagtggt gattaaacca gataatgtta
      361 agattttcct gcctagttat tagtcttgtg ttaaaattgc tttatgtcaa agactgattt
      421 gccattgaag gaaatgttac agcaagttaa agatttatct agtttgcaat tgttattgga
      481 agagaatgtt gctcaattgc ctttagccaa aaaacctcag gatattattc atggtgaagg
      541 tagtcaaaat gccgagatca tgttaattgg ggaagctccc ggttattatg aatcaattca
      601 gcgccgcccc tttgttggtc ggtctgggca attattacgt aaaactcttg aggaaattaa
      661 tcttccagca gaaaaagttt ttattagtaa tattattaaa gttagaccgc cagataatcg
      721 tgatccattg ccaaaagaaa ttttagcatt taaacctttt ttagataaag agatcgagtt
      781 aatcaaacct aaattgataa ttactctagg ccggtttagt ttggcaaaat ttttggaggg
      841 agtgaaaatc tcacaaattc atggacgcct acatagttta agatggcaag gtctaaactt
      901 gtttgtgttg cccatgtatc atccggcagc ggcactgcga tctagtcaag ttaaagaatc
      961 atttgttaac gattttaaaa aaattaataa aattttagct tggatagctg gtcaagaaga
     1021 aactcaaatt tttaaaaatc aattaagaga ggaattattt taatatgtca aaactcaaat
     1081 ttatagcctt aggaggcatg ggtcgagtta cccagaacct ttatttatat caatatgaac
     1141 aagaaatatt gttgattgat tgtggtattg gtttccctga tatttacatg cctggagttg
     1201 atgcgctaat cccagacatt acttatctgc tcaaccaaat tgaaggtgga gccaaaatcg
     1261 ttgggatgat tttaagtcat ggtcatgatg atcatattgg cgctttacct tatttattac
     1321 ccaatttgcc agattttcca atttatgctt cacccctaac agcgcagttt gctcatgatc
     1381 gtttattaga taaaaaagtt gataaaaaca tccaagtttt aggtgatcnn nnnnnnnntg
     1441 atcgacaaaa tttttaatat tggcccaatg aaaaattttg tcgatcaccc aaaacatcca
     1501 agttttgggt gatcgacaaa atttttcatt gggccaatat tttaaatttc aatttgttgc
     1561 gattactcat tctgttccag attcgaaaca tattttgtta gagactccgg tcggcaaaat
     1621 ttatcatggt agtgatttca aattggataa gcacccagtt gatggtcact taacagatat
     1681 ggaatttatt gaacaaattg gccgacaagg tgatttattg atggcttgtc tggattgtct
     1741 tcgagtagaa agaaatgaat ggacaccttc tgaatcaact gtaggcccag ctttactaga
     1801 agagatgaag ggagttaacg gcaaaatttt agtgacttta atgagttccc atctccaccg
     1861 cattcagcaa accgtgaatg cttgtcaaga attagggagg aaggtagtgt ttgttggtcg
     1921 gagtgttgag caaaatgtgc aaacggccat tcaatctaaa agtctcaata ttcctaaagg
     1981 tttaatggtt gataagaaaa agatcaaaga aattccggat gaaaaactct gtattattat
     2041 tgctggcagt cagggtcaag agggttcgtc tttggtcaga gcagtttatg gagatcacca
     2101 gattgtacaa attagtaatc gcgataaagt aattttttcg gctgatgtga ttcctggtaa
     2161 cgaaattcca tattactcag cggtagatca gttggcagct gatgaagtag acgtcgttta
     2221 tccagaaatt cgtccaagca ttcatcgttc aggtcatgct agtcgagttg aacaggagct
     2281 aatgttgaaa ttacttagac caaaatttgt gatgcccatt ggtggagctg atcgccatcg
     2341 agctttattc aaaaaaacag tggctaaacc gttgtcttat caagattggc aaatcctctt
     2401 accaaaaagt ggtgaagttt tagcaattga tcaacaaaga gtttcagtgc ttcagcaaat
     2461 atctttatca gctcaaaccg ttgatggttt aggtattggt gatgtggggc cgattgtttt
     2521 atctgaccgt ttaaatttat ctaaagcagg tattattgtt ttgcttttac cacgctttaa
     2581 gaaaaaaatt gatttcagca gaatgcaagt tatttctcga ggttttgttt ttatgaaaga
     2641 agccgacgaa gtggtgaact atattaaaaa agaaacggtg caaattgtgc aagatcttgg
     2701 taagaatgtc acagacgaag aacttaaacg gcgcttggaa aagcgtttgg cgcgtcgttt
     2761 attcaaagta attcggcgcg aaccaataat cttaccggta atctgggata tttaagggta
     2821 aaaaatattt attttttagc cttactgctt gcagttttca gacttttgtc ttttaataga
     2881 ggatagttaa tctgatcaaa atatggcaaa aagaaagtcc cgtcgtaaaa aacgtaaagc
     2941 gattcgcatg aaaatgcggg ccgacactac ctattccatc attgccgttc tactcattat
     3001 gtttggtagt ttggttttag tctcttttac tgggcaagga caatggttaa ttaccctgaa
     3061 taattttttg gcaaataaac ttggcttatc aatgttattt ttaccatttg tttttattgc
     3121 gtctggactg gtaatgatcc gcagcaagca cgcttggtct aaacccacca ttttgcttgg
     3181 cgtgattgtg atgatgattg gcgcgatggg agtgtctcga agtggagaaa ttggtataaa
     3241 tattttcaac aatttagctc tcttaattag tgctgttggc agttatattg tttatggagc
     3301 gctaactatt attggttttt taattctttc tcaaagttct ttgactgatc tggttaatgg
     3361 tatcagtaaa ataattagta aaaaccatca aaaaaaatca gctaaaaaag atgttaagac
     3421 tcttgataaa gatattactg atcaagaaat taaaaaatcg cggcactttt tcatccccaa
     3481 gttagctttt ggcaaaaagg atttaaaaag aggaagtgat tttcagaaag aaattgagct
     3541 ggatcaaact agtagtacgg cggttttaac tgagaaaaag gtaatagata ataaactccc
     3601 ctcaaatagt ttagacggag aggaagcatt aaatgcttcg tcaacgccaa tgatttggga
     3661 ttatccgccg ttgtctttgc tgactgtgga aaaaggagca aaagctgacc gtggtgatgt
     3721 taaagccaat gcacaaatta ttgaaaatac actagattct ttcggcatta atgccaaagt
     3781 agtcgaatac aatcctggtc ctgctgtcac tcagtactgt ttggagatta gtcagggtac
     3841 tcgtttggct aaaattactg ccctgtccac tgatttagcc ctagccctgg ccgcacctac
     3901 cggacaagta agaattgaag cgcctattcc aggtcgaaat ttagtgggag tagaagtacc
     3961 aaatcatagt gcggaaattg tgccactgaa aacaatgttg tcttcaccaa tacttaaaaa
     4021 acatccttct aaattagcgg tcagtttagg tgttgatgta gctggtaagc cagttgtggc
     4081 tgacatctct gaaatgccac acctcttgat tgctggtgcc accggttctg gtaaatcagt
     4141 ttgtgttaat gctttcttgt gttcaatttt atttagggcc actccagaag aagtcaaatt
     4201 aattttggtg gacccaaaac gggttgaact tggcaaatat catgatattc ctcatttgtt
     4261 aacaccagtc attcatgatc ctgacaaggt aatttcagct cttaaatggg cggttgaatt
     4321 aatggaaaaa cgctataaat tattaaatga agttggggtc cgttcaattc atgactacaa
     4381 tgagctagct ggtttagcta cgatgccaaa cattgtaatt gtcattgatg aaatggccga
     4441 cattatgctt tttgcgccta aagaagttga agataatgtc actcgtattg cacaaatggc
     4501 tcgtgcagta ggtattcatc tagtgttagc cactcaaaga ccatcggtag atgttatcac
     4561 tggtttaatc aaggccaata tgccaactag aattgctttt aatgtatcaa gcgtcactg
//
LOCUS       DAOSEW010000057         4521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_17231_length_4521_cov_3.873041, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000057 DAOSEW010000000
VERSION     DAOSEW010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17231_length_4521_cov_3.873041"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /locus_tag="PLJ02_02645"
     CDS             complement(<1..1108)
                     /locus_tag="PLJ02_02645"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI05337.1"
                     /translation="MSLNTFKVATRALLVNKSRTFLTTLGIIIGVFSVVMLTGLGSGL
                     QAYVSDQFEGLGANTVFVSIGQVFDGEGSFNRGSVGQILSGNKFTLRDIERIKRLRDY
                     VDEVTYFSTNVDEAEFSDNTTPVTILGTLANYPEAIDIDMASGRFFNVNEERSGEKVV
                     VLGDGVAQELFDQIDPVSRKIRIGNQNYRVVGVAAKQGGGFGGPSVDDYVYVPYTLTS
                     RIYQDTDITQFSVKIKSAEMIPEAITAIENELKRSFEEDEFSVFDQSQLLSTINNILS
                     MLTVALGGIAAISLLVGGIGIMNIMLVSVSERTREIGLRKALGATPNEIMIQFLIEAS
                     LLSVFGGLIGLGLAYLGAWALQPIFPIKVTLTAVL"
     gene            complement(1099..1821)
                     /locus_tag="PLJ02_02650"
     CDS             complement(1099..1821)
                     /locus_tag="PLJ02_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000811476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI05338.1"
                     /translation="MAKTTETVKAILELKQVSKIYTLGQQLIPALNKLSLKVCEGDFV
                     VIMGASGSGKSTLLQVASMLSQATEGEIYLKGKNVTEYDEVERARLRNKEIGFIFQQF
                     NLLAKTSAVEQVELPLIYAKVAEEERRERAIEMLKKLGLGDRLANKPGELSGGQQQRV
                     AIARALVNNPSIIFADEPTGNLDSKSGAEIKKILIKLNKEGKTIILVTHDPNVAKIAK
                     RQILIKDGQIIKDVRTDGKKCH"
     gene            complement(1831..2880)
                     /locus_tag="PLJ02_02655"
     CDS             complement(1831..2880)
                     /locus_tag="PLJ02_02655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOI05339.1"
                     /translation="MTKEKKSTKSKKFFSNKKTQRITIAVVLVIIVVSIFLNRGRSQQ
                     VEFSEYQVNRQDISQVLVLSGTLDAKERVYLNFLAGGKLVYLGAQEGDWVKQGQTIAT
                     VDYRDLQKSLEKNLNFYENRRLDWDAQLEEDKDQVLTEDEQRAREQNQNLLENTVLDV
                     ELISVAVDNRVMSAPFTGILLTAPVPVTGVTLSPTDLFELVNPQTLIVRAEVDEIDLS
                     LLSLGQKAKLIFDAYPEENIDSEITFIAPKSTVSSSGTVFEIELPVPGVVDLSKYRIG
                     MNADVEIELAKKTDVLVVPLEAVSNQDGQTIVKVKSQNSQGYEDRVIELGLESDELAE
                     VVGGLAEGEVILIED"
     gene            3066..3560
                     /locus_tag="PLJ02_02660"
     CDS             3066..3560
                     /locus_tag="PLJ02_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05340.1"
                     /translation="MKKTNCFLYSQQIKDLAKKGATLHKNLPHLPKNWLKNIVKILPY
                     LVLFEGVFSLTSGLSSLLVFDRDRAWAMHWLKINQAYYYVTAGFSIILAVFYLLAYEP
                     LKNKKYEGWLLLFWAIIASLIQSVVLLIMGWGGLFSSLIATLIGFYLIYEIRPEFITK
                     KKTK"
     gene            3563..>4521
                     /locus_tag="PLJ02_02665"
     CDS             3563..>4521
                     /locus_tag="PLJ02_02665"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015752946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HOI05341.1"
                     /translation="MTYEEIAKLLDHEAKYLLTHRCENLPKDLIYQVNPNQVREIFSQ
                     SDRSQSVIENLEKIYGHGRLANTGYLSILPVDQDIEHTASFSFYKNPLYFDPENIIRL
                     ALEAESNAIASSLGALALLSKKYADRIPFIVKINHNELLTYPNKWDQTLFAQVKQAYE
                     LGAVAIGATIYFGSPESNRQIREVSAAFYQAHQLGMATILWCYPRNSAWETKDADYNS
                     SADITAQANRLGVTIEADIIKQKMPTIDGAFRHFKFAKYSDQMYETLSTENPIDMLRL
                     QVINCYAGKIPLLNSGGESATEADFTKDLKAAVKSAVINKRAGG"
BASE COUNT         1386 a          958 c          724 g         1453 t
ORIGIN      
        1 ataaaaccgc cgttaaagta accttaattg gaaaaatagg ttgcagtgcc caagcaccca
       61 aataggccaa ccccaaacct attaatcctc caaaaacaga taagagtgat gcctcaatta
      121 aaaattgaat cataatttca ttgggtgtag cacccaaagc tttgcgcaaa ccaatctctc
      181 tagttctctc cgaaacggaa accaacataa tattcataat gccaatccca cccaccaaca
      241 aagaaatggc agcaataccg cccaaggcta ccgttaacat tgataaaatg ttattaatcg
      301 tgcttaacaa ttgagattga tcaaaaacac tgaactcatc ttcttcaaaa ctgcgcttta
      361 attcattttc aattgccgta atggcttcag gaatcatttc tgcgctttta atttttactg
      421 aaaattgggt aatatcagta tcttgatata tccgactagt caaagtatag ggaacataaa
      481 cataatcgtc gacactggga ccaccaaaac caccgccttg tttggctgcc actccgacca
      541 ctctataatt ttgattacca atacgaatct tacgagaaac tggatcaatc tgatcaaata
      601 gctcttgagc tacaccatct cccaaaacca ctactttttc tcctgaacgt tcttcgttaa
      661 cattaaaaaa tcgaccacta gccatatcaa tatcaattgc ttctggataa ttagctaaag
      721 tacccaaaat tgtgactggt gttgtattat ctgagaattc tgcttcatca acattagttg
      781 aaaaataagt cacttcatca acataatctc tcagtctttt gatgcgttca atatctctta
      841 aagtaaattt attgcctgat aaaatctgac ccacactacc ccgattaaaa ctaccctcgc
      901 catcaaaaac ttggccgatt gatacaaaca ccgtattggc tcccaagcct tcaaattgat
      961 cggacacata ggcttgcagg ccactgccta aaccagttag cattactacc gaaaacactc
     1021 caataatgat ccccaaggtc gttaaaaagg ttcgactttt gttgaccaat agagctctgg
     1081 tagctacttt aaaagtgttt aatgacattt ttttccatct gttctgacgt ctttaatgat
     1141 ttgtccgtct ttgattaaga tttgacgttt ggcaatttta gccacatttg gatcgtgagt
     1201 caccaaaata atcgtcttcc cttctttgtt taacttaatt aaaattttct tgatttccgc
     1261 accagattta gaatcgagat taccagttgg ttcatcggca aaaataatac ttggattatt
     1321 caccaaagct cgagcaatgg ctacccgctg ttgctgtcct ccagacaatt ctcctggttt
     1381 attagccaaa cgatcgccca aacccaattt ctttaacatc tcaatggcgc gctctctgcg
     1441 ttcctcttca gcaactttgg cgtaaattag tggtaattca acttgttcta ccgctgaggt
     1501 tttggctaaa agattgaact gctgaaaaat gaaaccaatt tctttattcc gtaatcgtgc
     1561 ccgttccact tcatcatact ctgtcacatt tttacctttt aaataaattt ctccttcagt
     1621 ggcttgtgat aacatgctag ctacttgcaa caacgttgat tttcctgaac cagaagcacc
     1681 cataataact acaaaatcac cttcacaaac tttgagtgat aatttattta atgctggaat
     1741 taattgttga cctaaagtgt aaattttaga aacttgtttc agctctaaaa tggctttaac
     1801 tgtttctgta gttttagcca taattaaatc ctaatcttcg ataagaatca cttcaccttc
     1861 tgctaaaccg cccaccactt ctgccagctc atcactttcc aaacctaatt caattaccct
     1921 atcctcgtaa ccttgactgt tttggctttt aaccttaacg atggtttgac catcttgatt
     1981 actaaccgct tccagtggca ccactagaac atcggttttt ttggctagtt cgatttctac
     2041 atcagcattc atgccaatcc gatatttact caaatccact actccgggta ctggtaattc
     2101 aatttcaaag actgtgcctg agctgctaac agtacttttt ggcgcaataa aagtaatctc
     2161 actatcaata ttttcctctg gataagcatc aaaaattaac ttagcttttt gaccaagact
     2221 caacaatgat aaatcaattt catcaacttc ggctctgact attaaagtct gaggattaac
     2281 caactcaaaa agatcagtgg gtgataaagt tacccccgtc acaggaactg gagcagttaa
     2341 taaaatacca gtaaaaggag cactcatcac tcggttatca acagcaacac taattaattc
     2401 cacatcaagg acagtgtttt ctaacaaatt ttgattttgt tctcttgccc gctgttcatc
     2461 ctcggtcaaa acctgatctt tgtcttcttc caattgggca tcccaatcta gccgccgatt
     2521 ttcataaaaa tttaagtttt tttctaaact tttttgtaaa tctcggtaat ccactgtagc
     2581 aatggtttgt ccttgcttaa cccaatctcc ttcttgagcc cctaaataaa ccaattttcc
     2641 gccagctaaa aaattaagat aaacccgctc cttagcatct aaagttccag ataagaccaa
     2701 cacctgacta atatcttgac gattcacctg atattcactg aattccactt gttgactgcg
     2761 tccacgattc aaaaaaattg agacaacaat aatcactagt acaactgcaa tggtaattcg
     2821 ctgtgttttc ttattgctaa aaaatttctt agatttggtg cttttttttt ccttagtcat
     2881 ggaggtaatc ctgctgttgt attatattat cagttctttt cgttttttaa tagatcgtac
     2941 aaaataccaa aaatatgtgt ttttggcaag ctataaaaaa ttcacactgt aattttcttc
     3001 tgcgttagaa ggtttcttta tccttgacta ttaaaaaaat tagttttttg ctagaatgaa
     3061 ttatgatgaa aaagacaaat tgcttcctct acagtcaaca aatcaaagat ttagctaaaa
     3121 aaggagcaac tctacataaa aatttgcctc atttgcccaa aaattggcta aaaaatattg
     3181 ttaaaatttt gccttatttg gttctatttg agggagtttt tagtctgact tcgggtttaa
     3241 gcagtttatt ggtttttgat cgcgatcggg cttgggcaat gcactggctg aaaatcaatc
     3301 aggcctacta ttatgtgact gcgggattta gtattatttt agcggttttt tatttgctag
     3361 cttacgaacc actaaaaaat aaaaaatatg agggctggct cctactgttt tgggcaatta
     3421 tcgcttcact aatccagagt gttgttttac taattatggg ttggggtggt ttgtttagct
     3481 ctttaattgc tacgctaatt ggtttctatt taatttacga aattcgtcca gaatttatta
     3541 ctaaaaaaaa gactaaataa acatgactta cgaagagatt gctaaactct tagatcatga
     3601 ggccaagtat ttgctcacac atcgttgtga aaatttgcct aaagatttaa tctaccaagt
     3661 caatcccaat caggttagag agatcttttc gcagtctgat cgcagtcagt cagttattga
     3721 aaatttagaa aaaatttatg gtcatggtcg tttagcaaac acaggctatt tatcaatttt
     3781 acctgtagat caagatatcg agcacaccgc cagtttttca ttttacaaaa atcctcttta
     3841 ttttgatcca gaaaacatca ttcgcttagc tttagaagca gaaagtaacg ctattgcctc
     3901 tagtttgggt gcgttggcat tgctgagcaa aaagtatgct gacagaattc cttttattgt
     3961 taaaatcaat cacaatgaat tactcactta tccaaataaa tgggatcaaa ctctctttgc
     4021 tcaagtaaaa caggcttatg aattgggagc agtcgccata ggtgcgacga tttattttgg
     4081 ttctcccgaa agcaatcgtc aaataaggga ggtgtctgca gcgttctacc aagctcacca
     4141 gctcggcatg gccactattc tttggtgtta tcctcgtaat agcgcctggg aaactaaaga
     4201 tgctgactat aattctagtg ctgatattac tgctcaggcc aatcgtctag gcgtcacaat
     4261 tgaagcagac atcattaaac aaaaaatgcc aaccattgat ggtgcttttc gtcatttcaa
     4321 attcgccaaa tacagtgatc aaatgtatga gacactttca actgaaaatc ccattgatat
     4381 gttgcggctt caggtgatta attgttatgc tggtaaaatt cctttactca attctggtgg
     4441 ggaaagtgct accgaagctg attttacaaa agatttaaaa gctgccgtca aaagtgcggt
     4501 gattaataaa cgagctggcg g
//
LOCUS       DAOSEW010000058         4484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_17465_length_4484_cov_4.887333, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000058 DAOSEW010000000
VERSION     DAOSEW010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17465_length_4484_cov_4.887333"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="PLJ02_02670"
     CDS             <1..1000
                     /locus_tag="PLJ02_02670"
                     /inference="COORDINATES: protein motif:HMM:NF012796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HOI05342.1"
                     /translation="NWLTTGGWDEIDVLFKLKSRTNREYALGQSHEEIVTPLAKELIS
                     SYKDLPLALYQIQWKYRDELRSKSGILRGREFYMKDMYSFHADQADFEKFYQETKNVY
                     LKIFEKLNLPVKVTEASGGSFTDKISYEFMVITDAGEDDILYCDDCSYCINKEIAKVK
                     VGSPCPKCGSALKQARASEVANVFDLGQKFTKAFKLQFTDQNGQQQFPIMGCYGIGIS
                     RAMGVIVEKHHDQKGIIWPKAVTPYQAHLISLEGTETFARKIYDDLLKQGVEVLWDDR
                     QRSAGEKFADSDLIGIPIRLVVSKRNQNQIEWQERGQNKVELIGSNELLSRLKINK"
     gene            1233..2138
                     /locus_tag="PLJ02_02675"
     CDS             1233..2138
                     /locus_tag="PLJ02_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05343.1"
                     /translation="MPQRKLLVTHHAPDLDAIGGIWLFKRFDALNYANAQVAFVDPGD
                     TISLEDCEKNFNCQLHEVTHVDTGLGEFDHHQPEKAQASISASSLVYDYICQQYPDKK
                     DDQALAALVNHITEIDHFREIYWAEAKEDRFLFSLHSLIHGHEYVNLHDDQSQLNFGL
                     QSLDYAYAALTQHYKAKEAIKERGQEFNLPIGKCLAIESGNDDTIKLAQIQGYQLVIR
                     KDPEQDFIRIKARPDSKNPDGSNFNLEKLAKIIKEIDQSASWFYHASGKMLLNGSNGW
                     RQKKASKLSLQQVVDLVQKTYNQKS"
     gene            2176..2523
                     /locus_tag="PLJ02_02680"
     CDS             2176..2523
                     /locus_tag="PLJ02_02680"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOI05344.1"
                     /translation="MTESVFTKIIKRELPSTIHYEDDQFIVISSNDPKAPVHLLIITK
                     RAYQNLEEVDPENQELHAKMLLLCRKMAQEKGISDNYQIHINVGKAMQQVQHLHIHLL
                     GGWKNPEKVKQRF"
     gene            complement(2520..3188)
                     /locus_tag="PLJ02_02685"
     CDS             complement(2520..3188)
                     /locus_tag="PLJ02_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOI05345.1"
                     /translation="MRLLIIEDDLILSDLLVNYLLKSYLVDLTTNLHKIENLFDQKSY
                     QAILLSSHVFYSQELKKRAGRVFAKTQAPVLLLTNGQISQQDFGFLGQRLIDFLHKPF
                     GSDELNLRLGVILFNLSGEKQETCLRYKNLLLDNRSHCLVCGKKSVSLTKKEFYLLQL
                     FFKRPKQVFSKSLLANLVWDDEEVIYGNTIATHLSSLRKKIKSISGQDLIKTARGEGY
                     FLSD"
     gene            3339..3599
                     /locus_tag="PLJ02_02690"
     CDS             3339..3599
                     /locus_tag="PLJ02_02690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOI05346.1"
                     /translation="MINLPVIIKAKKNQSTADVIRQFKKASASAGTVQIAKDRRYFAK
                     PSRIKADKTAERSRLKKRAQSLKKRKNVSPAAIARIQQRLGS"
     gene            complement(3658..4161)
                     /locus_tag="PLJ02_02695"
     CDS             complement(3658..4161)
                     /locus_tag="PLJ02_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05347.1"
                     /translation="MSDDLVKKALASDEDEAVEKVVAEVKKTVTSDDDEVEKSDQLAE
                     TLISLQNLIERHALELTKIDSELSEKREAIKSVFDNDTLLAEKKEELDQYNQQYKERK
                     SQIQNNPQVIALKVDVADLTEQKKDLEETLSNHLINYHSLTNSTSFDTSDGDQWEFVI
                     KAKIKKK"
BASE COUNT         1495 a          805 c          795 g         1389 t
ORIGIN      
        1 caattggttg actactggcg gttgggatga aattgacgtg ctctttaaat taaaaagtcg
       61 caccaaccga gaatatgcgt tgggtcagag ccacgaagag attgtcacac ctttagctaa
      121 ggagctaatt agctcttata aagatttacc cctagccctt tatcaaattc agtggaaata
      181 tcgtgacgaa ttacgctcta aatcaggtat cttgcgtggt cgagaatttt acatgaaaga
      241 tatgtatagt tttcatgctg accaggcaga ttttgaaaaa ttttatcaag aaacaaaaaa
      301 tgtttattta aaaatttttg aaaaattaaa tttgccagtc aaagtcaccg aagccagtgg
      361 tggcagtttt actgataaga tttcttatga atttatggtc atcactgatg ctggcgaaga
      421 cgatatttta tattgcgatg attgcagtta ttgtattaat aaagaaatcg ccaaagttaa
      481 ggtcggcagt ccttgtccga agtgtgggtc agctctcaaa caagcgcggg ctagtgaagt
      541 agctaacgtt tttgatttgg gtcaaaaatt tactaaagct ttcaaactgc aattcactga
      601 tcaaaatggt cagcaacaat tcccaatcat gggttgttat gggatcggga ttagtcgagc
      661 tatgggcgtc attgtggaaa aacatcacga ccaaaaagga ataatttggc ccaaagcggt
      721 tactccatat caggctcatc ttattagtct cgagggcaca gaaacatttg cccgaaaaat
      781 ttatgatgat ttactcaagc agggtgtaga agttctctgg gacgatcgac agcgctccgc
      841 tggagaaaaa ttcgctgata gtgatttaat cgggattccc attcgtttag tagtttccaa
      901 acggaaccaa aaccaaatcg agtggcaaga gcggggacag aacaaagtcg aattaattgg
      961 atcaaatgaa ttgttaagtc gtcttaaaat aaataaatag tcaaaaatag tccttttttg
     1021 caataattgc ggtaaaaatt aatcctatat ttaaataatt tattaatcaa cagaaaaatt
     1081 ataaacctaa taatcaagct ttttttctta tttaaaaaag caaaaaagat tatatattaa
     1141 ctaatgttta tcggtgagga agacccccac tgaaatctac ctactaggcg atttcggtgg
     1201 gttctttttt tagctgctgg tataatgttt ctatgccaca aagaaaactc ttagttactc
     1261 accacgctcc agatttagat gcaatcggtg gtatttggtt gtttaaaaga tttgatgccc
     1321 tcaattatgc taatgctcaa gtagctttcg tcgacccagg tgacacgatt agcttagagg
     1381 actgtgaaaa aaattttaat tgccaattgc atgaagtcac tcacgttgat actggcctgg
     1441 gtgaatttga ccatcatcaa cctgaaaaag cccaagcatc aatctctgct agttctttgg
     1501 tctatgacta tatttgtcaa caatatccag ataaaaaaga tgatcaggct ttagccgccc
     1561 tagtcaatca cattactgag attgatcatt tccgagaaat ctactgggca gaagccaaag
     1621 aagatcgttt cttattctca ctacattcgt taattcatgg tcacgaatat gttaatctac
     1681 atgatgatca atcccaatta aattttggct tacaatcatt agattacgct tatgccgctt
     1741 taactcaaca ctataaggca aaagaggcga ttaaagaaag gggtcaagaa tttaatttac
     1801 ctattggtaa atgtttggcc attgaaagtg gcaatgacga caccatcaaa ttagcacaaa
     1861 ttcaaggtta tcaattggta attcgtaaag acccagagca agattttatc agaattaaag
     1921 ctagacctga cagcaaaaat cccgatggga gtaattttaa tttagaaaaa ttggccaaaa
     1981 ttattaaaga aatagatcaa tcagctagtt ggttctatca cgccagtggc aaaatgttac
     2041 ttaatggtag taacggttgg cgacaaaaaa aggctagcaa acttagtctg caacaagttg
     2101 tcgatttagt tcaaaaaacc tataatcaaa agagttagca gatcatcaaa aaagttagtt
     2161 tgaaagaaag ccatcatgac cgagtcagtt tttactaaaa ttattaaaag ggaattgcct
     2221 agcaccattc attacgaaga cgatcaattt atcgttattt ctagtaatga cccaaaagct
     2281 ccagtgcact tattgataat tactaaacga gcctaccaaa atttagaaga agtagatcct
     2341 gagaatcaag agttacatgc caaaatgttg ctactttgcc gaaaaatggc tcaagaaaaa
     2401 ggcataagtg acaattatca aattcatatt aatgttggta aagccatgca acaagtgcaa
     2461 cacttacata ttcatcttct aggaggttgg aaaaatcctg aaaaggtgaa acaaagattt
     2521 taatcgctta gaaaataacc ttctcctcgc gcagttttaa tcagatcttg accagaaatg
     2581 cttttgattt ttttgcgtaa actactcaaa tgtgtagcaa tcgtatttcc ataaattact
     2641 tcctcatcgt cccacaccaa attggccaaa agagatttag aaaacacctg tttaggtctt
     2701 ttaaaaaaaa gttgtagtaa atagaactct tttttggtta atgaaacact tttcttgcca
     2761 cacaccaaac agtgagatct attatcaagt aacaaatttt tatatcttag acacgtttct
     2821 tgtttttctc cagataaatt aaataagata acacctagcc gcaaatttaa ttcatcactg
     2881 ccaaatggtt tgtgtaagaa atcgatcaat cgttgaccca aaaacccaaa atcttgttga
     2941 ctaatctgac cgttagtcaa tagtaatact ggtgcttgag tctttgcaaa aacccgacca
     3001 gctcgttttt tcaattcttg actataaaaa acatgagaac tgagcaaaat agcttgataa
     3061 cttttttgat caaataaatt ttcaatttta tgtaaattag tagttaaatc tactaagtag
     3121 ctttttaaaa gatagttaac taacaaatct gacaaaatta agtcatcttc aataattaac
     3181 agacgcataa ctgctttata ttttttaaaa aactttttca aagttaacat taaaacttaa
     3241 atgttgtcaa ttagaaagtt ttccaaatta actctggttt tattgccctc tatcacttat
     3301 aatggttccc tctaatcagt aaataactca gagagggggt gattaacttg ccagtaatta
     3361 ttaaagcgaa aaaaaaccag tcaacagctg atgtcattcg ccaattcaaa aaggctagtg
     3421 cgagtgctgg tacagtccaa atcgctaaag accgtcgcta ttttgccaaa ccctcaagaa
     3481 ttaaggctga taaaaccgct gagcgaagtc gattaaagaa acgtgctcaa tcattaaaaa
     3541 agagaaagaa tgtttctcca gcagccattg ccaggattca acagcgcctt ggttcctaaa
     3601 tgattttgca aaaaaataaa cccgcttaac aagcgggttt tttattattt aagaaaatta
     3661 tttcttttta attttagctt taatcacaaa ctcccactga tcaccatcgc tggtatcaaa
     3721 actagtcgaa ttggttagac tatgatagtt gattaggtga ttactcaagg tttcttctaa
     3781 atcttttttt tgttctgtca aatctgcaac atccacctta agagctatga cttgaggatt
     3841 gttttgaatt tggcttttgc gttctttata ttgttggtta tattgatcga gttcttcttt
     3901 tttttcggct agtaaagtat cattgtcgaa cacacttttt atagcttccc ttttttcgct
     3961 taattcgcta tcgattttag ttaactctaa agcatgacgc tcaattaaat tttgtaatga
     4021 gattaaagtt tcggctaact gatcagattt ttcaacttcg tcatcatcac tagtcactgt
     4081 ttttttgact tcggctacaa ctttttctac agcttcatct tcgtcgctgg ccaaggcttt
     4141 tttcactaaa tcatcgctca taatttcctt tatttttaat aatctagctg acagattagc
     4201 agaattttta gtataataca agagaacatg gccgagtaat gggtcattga tgaaaatctt
     4261 tgacaggaaa gtccggacag cagagtagag ggtgcgtgat gaaaatcacg actagtggca
     4321 acactagcta gttagagcaa cagcgacgaa ctgggtagcg ccagctgaaa cgatcaagct
     4381 tttggataga caatcagcaa tgattgtttg gcaatcctcc ccgctgcgaa caacgacttt
     4441 gaccttaatg gatctactcc ctatatttcc ttcgggaaag atgg
//
LOCUS       DAOSEW010000059         4451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_17668_length_4451_cov_4.843722, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000059 DAOSEW010000000
VERSION     DAOSEW010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17668_length_4451_cov_4.843722"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PLJ02_02700"
     CDS             complement(<1..238)
                     /locus_tag="PLJ02_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05348.1"
                     /translation="MGSKKNVDMTQTESDVKIIKAETEKEAGAEKKAAAKEETKAKKE
                     TGAEKKAAADGKKEAKAKKEAKAKKEAGAEKEATD"
     gene            complement(322..747)
                     /gene="rplK"
                     /locus_tag="PLJ02_02705"
     CDS             complement(322..747)
                     /gene="rplK"
                     /locus_tag="PLJ02_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOI05349.1"
                     /translation="MAKKVKQVLKLNLPAGNATPAPPVGPILGQAGINMMDFIKQYND
                     QSADKRGQIIPAEVTVYEDRSFSFVLKLPPVSDLIKQKIKLKKGSALAGRENKGKLTK
                     AQVREIAEEKLADLNTTDIEAAMRTVIGAAQSMGVDIVD"
     gene            complement(761..1501)
                     /gene="nusG"
                     /locus_tag="PLJ02_02710"
     CDS             complement(761..1501)
                     /gene="nusG"
                     /locus_tag="PLJ02_02710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOI05350.1"
                     /translation="MAKRQDSKNTTKTAKKTTAKSKKVDQTQDAPVVADQEVKKQAKA
                     EQQTDAQETSKTKQKIEDNLIVISQETNKKARWYVVHVYSGHEKKVAEALRQRTETLH
                     LLDKILRVFIPTQDKIQIRRGQRRTVSEKIFPGYILVEMEMTDNAWLAIRTTEGVTGF
                     VGMSSRPTPLPKSEVEAIMKYAEQSDTQYKADFVDGEAVRITDGPFNDFLGTVDNIDE
                     ERGKVTVLINIFGRETPVELDFLQVKKV"
     gene            complement(1549..1731)
                     /gene="secE"
                     /locus_tag="PLJ02_02715"
     CDS             complement(1549..1731)
                     /gene="secE"
                     /locus_tag="PLJ02_02715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOI05351.1"
                     /translation="MLQAIKKYLLEVKTELGKTTWPDKKTTKNLSILVVVVSLLLALY
                     IGFFDFILQELIALFV"
     gene            complement(1746..3749)
                     /locus_tag="PLJ02_02720"
     CDS             complement(1746..3749)
                     /locus_tag="PLJ02_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05352.1"
                     /translation="MEENYLQKSRKKPFWSYFFFVLGVLVIIFILSATLFGLIFLQSV
                     DENLDIRKQALEVSKRVDFDTYPFAQSFFKVQEPSRIELQINTYNHYVDALELGLQIK
                     ADQGLLTASDLQITASNDQVLKLVQTDVQEITCDPQQDCFLITLLLITHQINEPFTSH
                     NVSTSIANLEFIPQKEGAITLELTPASQVNEHGTGQNILQNPSATQFDYYVSNNGIDF
                     AQCHYVYGDWTECENFWQTRQYEVAPLNCHWHQTETLETLSRQCPDNPESNSSLAETK
                     DLALHYFASCLSNHSQGENFYLVWNTELYPGFDWLDVSLDHRFNSFYQKSTKTAGAIG
                     NYAIIDLKGLTGFSQDVRGQILNFQADQNYYFRLYNDQTEEFVYGPKLYLNYCNQEQS
                     TRLDCQEACIQTNQGSNCQNGLSCIDGRCVLESNPENPWCFDEKLMSKQNRLGSVAGV
                     STQVADTNVIIQGPFLPSEVYDLTTYACNHGCNTNRDCMADYRCYQGRCRLASNPESP
                     TCEAATVVDDSEPIEQKGGVIDDQPPIVDQPLPSNTPIPLPTQETVDNLATDSGGLGE
                     TTDTEPTSAEETASTEPEASSPAMFANLSATLQDFFNKFNWQYLALAGLLIIIAVVFI
                     FLGLSHNPRQAQAKPSKPNSQNTDNQKEVAEEVTIPENKELDL"
     gene            complement(3792..4196)
                     /locus_tag="PLJ02_02725"
     CDS             complement(3792..4196)
                     /locus_tag="PLJ02_02725"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HOI05353.1"
                     /translation="MTKKTKSYPEFIQRMDWPAIIFLLFLVAFLGGLTCQHYFRRQQQ
                     LRWQAIQDLQTAEMAKLNQLNITEDLLKKADLNQDGQINQTDTEIMQSSFLSIDSDSL
                     KADLNQDGRVDTKDYAILAHIISSAKTEDTQE"
BASE COUNT         1180 a          886 c          891 g         1494 t
ORIGIN      
        1 cgtcagtagc ctctttttca gcccccgcct cttttttagc cttggcctct ttttttgcct
       61 tggcctcttt tttaccatcg gctgcagctt tcttttcagc cccggtttct tttttggctt
      121 tagtttcttc tttggcagcg gccttttttt ctgctccagc ctctttttcc gtttcagcct
      181 tgataatttt gacatcactc tcagtttgag tcatgtcgac attttttttg cttcccataa
      241 ttcctttctg ttagcgtggt gcaaacgact ggggtaaagc ttaagtctgc cacactaata
      301 cttatttatt tttttaattt tttaatcgac aatatctaca cccatgcttt gggcagcacc
      361 gataactgta cgcatagctg cttcaatatc tgtggtattc agatcagcca gcttttcttc
      421 agcgatttct ctaacttggg cttttgttaa tttgcctttg ttttctcggc cagccaaagc
      481 gctgcctttt ttcagtttaa ttttttgctt aattaaatct gaaactggag gcagttttag
      541 cacaaagcta aaagaacgat cttcgtaaac cgttacctcg gctggaatga tttggccacg
      601 cttgtcggca ctttgatcat tgtactgctt gataaaatcc atcatgttaa taccagcttg
      661 acctaaaatt ggaccaactg ggggagcagg cgtagcattg cctgctggta agtttaactt
      721 gaggacttgt ttgacttttt ttgccatgac tttctttctt ctaaactttc ttgacttgta
      781 agaaatctaa ttcgaccgga gtttcccgac caaaaatatt aattaacacg gtcactttac
      841 cacgttcttc atcgatatta tcgacagtgc ccaagaaatc attaaatggt ccatcggtaa
      901 tacggaccgc ctcgccatcg acaaagtcag ccttgtattg agtatcactt tgttcagcat
      961 atttcataat tgcttcaacc tcactcttgg gcagtggggt agggcgacta ctcataccaa
     1021 caaaaccagt gactccttca gtggtccgaa tggctaacca ggcgttgtct gtcatttcca
     1081 tttccaccaa aatgtaaccg ggaaaaatct tttcgctaac agtgcgccgc tgaccgcgtc
     1141 taatttgaat tttatcttga gtaggaataa aaactcgtaa gattttatcc aataagtgta
     1201 aagtttcggt tcgttggcgt aaggcttcgg ccactttttt ttcgtgacca gaataaacgt
     1261 gaactacata ccaccgcgct tttttgttag tttcttggct aatgacaatt aaattatcct
     1321 caattttttg tttggttttg cttgtctctt gtgcgtctgt ttgttgctca gctttggctt
     1381 gctttttaac ttcttgatca gctacaactg gagcgtcctg agtttggtca acttttttgg
     1441 actttgcagt agtttttttt gctgttttag ttgtattttt gctgtcttga cgctttgcca
     1501 taaatgcctt ttgccttgct tttttttgcg agcgattatt ataccagatt aaacaaaaag
     1561 tgctatgagt tcttgtagga taaaatcaaa aaagccaatg tagagagcta ataaaagact
     1621 gaccaccacc actaaaatac ttaaattttt ggtcgttttt ttgtcaggcc aggtggtttt
     1681 tcctagttcg gttttgactt ccagtaaata ttttttaatt gcttgcaaca tagattggca
     1741 ttttatcaca gatcaagctc tttattttct ggaattgtca cctcttctgc gacctctttt
     1801 tgattgtctg tattttgact gtttggtttg cttggtttgg cttgagcttg acgaggatta
     1861 tggcttaaac ctaagaaaat aaagacgacg gcgataataa ttaataagcc agctaaagcc
     1921 aaatattgcc aattaaattt attaaaaaag tcttggagag tggccgaaag attagcgaac
     1981 atagctggcg agctagcttc aggctcagtt gatgcagttt cttccgcaga cgttggttca
     2041 gtatcggttg tttctccaag cccacctgag tcagtggcaa gattatctac tgtttcttga
     2101 gtgggtaagg ggatgggagt attgcttggt agtggttgat caacgattgg tggctgatca
     2161 tcaatcacgc cccctttttg ttctattggt tcactgtcat ctactacagt tgctgcttca
     2221 caagtcggac tctctggatt gctagccaaa cgacagcgac cctgataaca gcgataatct
     2281 gccatacaat cacgattggt attgcagcca tgattgcaag cataggtggt caagtcatac
     2341 acttcactag gcaagaaagg accttgaata attacgtttg tgtcggcaac ttgagtgctg
     2401 actccagcca cgctccccaa acggttttgc ttgctcatga gcttttcgtc aaaacaccag
     2461 ggattttcag gattactttc taaaacacag cgcccatcaa tgcaactcaa accattttgg
     2521 caatttgaac cttgattggt ttggatacaa gcctcttgac aatcgagtcg agtactttgt
     2581 tcttgattac aataatttag gtaaagtttg gggccataaa caaattcttc agtctgatca
     2641 ttgtagagtc gaaaataata attttggtca gcctgaaaat taaggatttg tccccgcaca
     2701 tcttgagaaa agccagtcaa tccttttaaa tcaatgatgg cataattacc aattgcccca
     2761 gccgttttag tcgatttttg gtaaaaacta ttgaagcgat gatcgagact gacatccaac
     2821 caatcaaaac caggatatag ctctgtattc cacactaaat aaaaattttc tccttgacta
     2881 tgattgctta aacagctggc aaaataatga agagccaaat cttttgtctc agccagactg
     2941 gaattgcttt ctggattgtc aggacattga cggctgagtg tttctagtgt ctcggtttga
     3001 tgccagtggc agttgagtgg cgctacttca tattgacgtg tttgccagaa attttcacac
     3061 tcagtccagt caccataaac atagtgacat tgagcaaaat cgataccatt attactgaca
     3121 taatagtcaa attgagttgc gctggggttt tgcaaaatat tttgaccagt gccatgttcg
     3181 ttaacttggc ttgcgggagt tagttccaaa gtaatggcac cttctttttg gggaataaac
     3241 tctaaattgg caatcgaagt ggacacattg tggctagtga acggttcatt aatctggtga
     3301 gtaataagta acaaggtaat caggaaacag tcttgttgtg gatcacaagt tatttcctga
     3361 acatcagttt gcactaactt taaaacttga tcattggatg cggtgatttg taaatctgag
     3421 gctgtcaata aaccttgatc agctttaatt tgtaagccaa gctcaagagc atcaacgtag
     3481 tggttgtaag tattaatttg caactcaatc cgactaggtt cttgtacttt aaagaagctt
     3541 tgggcaaagg ggtaggtatc aaaatcaacg cgtttgctca cttccagagc ttgtttacga
     3601 atatccaaat tttcatcaac ggattgtaag aaaattaaac caaagagggt ggcgctcaag
     3661 ataaaaatga ttactaacac gcctaataca aaaaaaaagt aggaccaaaa cggttttttt
     3721 ctagattttt gtaagtaatt ttcttccatt attgttcgtt tcttctattt ttcgttgcta
     3781 atactagtca attattcttg agtgtcttcg gtttttgctg agctaataat gtgggctaaa
     3841 atagcataat ctttggtatc cacgcgacca tcttgattga gatcggcctt tagactgtcg
     3901 ctatcaatgc tcaaaaaaga actttgcatg atctcagtat cggtttgatt aatttgacca
     3961 tcttgattga gatcggcctt ttttaataaa tcctcagtaa tatttaactg attaagtttt
     4021 gccatttccg ccgtttgtaa atcttggata gcttgccaac gcagctgttg ctgtcggcgg
     4081 aaataatgct gacaagtgag cccgcccaaa aaggcaacta gaaataagag gaagataatg
     4141 gctggccaat ccatgcgttg aatgaactca ggatatgact tagttttttt agtcattaac
     4201 taagcatact aaacttgatg gggaaaggtc aagcgctatg gggattttta ctagtaaata
     4261 tgttatactc gttggcgtta tgaccaaaaa aaaagtgaga aaagcgatta ttgccgacgc
     4321 tggttttgcc agtcgttatt tacccatcac taagacttta cctaaagcga tgttgcccct
     4381 gggtaaccgc ccgattatgc aatttgtgat tgaagagtgt gtggaagcag agattgacga
     4441 aattattgtg g
//
LOCUS       DAOSEW010000060         4211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_19039_length_4211_cov_4.932146, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000060 DAOSEW010000000
VERSION     DAOSEW010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19039_length_4211_cov_4.932146"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PLJ02_02730"
     CDS             complement(<1..205)
                     /locus_tag="PLJ02_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HOI05354.1"
                     /translation="MLLIKNKKAYFDYQIDKKYLAGIVLSGAEVKSLRLKHASLAGSY
                     IKALNKDSKKPELFLINAQINAYR"
     gene            complement(224..1981)
                     /locus_tag="PLJ02_02735"
     CDS             complement(224..1981)
                     /locus_tag="PLJ02_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05355.1"
                     /translation="MQELSLLPLLAKSQANYFVGFLKNRQENFIMVLSLQGPDAQFFG
                     KDLQIRLSTWQFTSAEKIHLALLDLNKECRLKKLQLNFALSLKVGKKIILACQGGSIL
                     LKRDQQLRELISSQSEISMLVGQYIENDQFVLIAGNYQLGKVIVEKNRNYPLELIIEQ
                     KLDDYFKNNFNTALLLWSYQHKEKVSWKEKLAKQKIILLKIKKIPQKIKEFVLKIKNL
                     RQEQKKHYQKIFLLVVLAIVLVTTALLSWRHLEQKNLDKANKEIDALVLSNAELEQLS
                     TKQPVLAREKIQENQEQLRQLLDEYNSLLAKKRIEEKIQELQARAEQLVSENNLDQLF
                     VYSNWYDNYPDFLGNKIIASPQGLLVGNSQTDRLLLVNNNNFDQWTINFADFSIDNSS
                     SDRLKIFIKDQGIKYLDWQDRSSFALKTEGDSDRDAQFVESYQNFLYLLNPEKRNIYR
                     YTLTNGELSEAIGWLVDKQNINFSTVSDMSIDGDLWLGFKDGKIMKFSRGYQVDFALT
                     GLEQLPTDLVKIASQENSDKIVFLDKSNNRLIVSTKEGQLISEIKSNELAGVSDITLA
                     NDGQSCFALSGSVIYQIQI"
     gene            complement(1987..3183)
                     /gene="tsaD"
                     /locus_tag="PLJ02_02740"
     CDS             complement(1987..3183)
                     /gene="tsaD"
                     /locus_tag="PLJ02_02740"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOI05356.1"
                     /translation="MGKKPTLAKKKKILAIDTSCDDTSVAILEGARVLANVVASQTNI
                     HQQYGGVFPTLAKQAHQANIEACTKTALHRAGFNDESKIKDQLQAVAVTVGPGLAPAL
                     EVGINYAKKLAKMWQLPIIPVNHIEAHALSPLLQPKTQNLSRTINFPVLAMVISGGHS
                     EFILIKKSGDYERLGWTVDDSAGECLDKIGRMLDLGYPAGPILEKLAKKGNAKRWSLP
                     LAMTNVENFNLSFSGFKTATHQLIKQLKKEEKLDAQAVCDLAAIVQQQVFAQINYKLE
                     KILFSPDLNQKIYPKAQLSKKIKQGKIKPPITTINELWLGGGVAANIALRNSIRHTLK
                     NYQKLSAKKINLYLPYHKKFCADNAAMIALTAHLTLKRGVTTFEGKLDRQPNLAIGAA
                     LPSMLE"
     gene            complement(3162..>4211)
                     /locus_tag="PLJ02_02745"
     CDS             complement(3162..>4211)
                     /locus_tag="PLJ02_02745"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOI05357.1"
                     /translation="RLIDQAIAVIKNGGLVIYPTETTYGIGANPHSTPAIDKLLQYKS
                     RREGKPLSIAVNSQTMAEQYVKINQQAQNFYQRFLPGPYTVISQLKKTVDIDKRVASE
                     FGTLGIRIPDYPLILKLIATLGHGITATSANASNQKRPYQISDILDNLSEKQQQLIDL
                     IIDVGRLPKNEPSVVIDTTLSTPLALRGQLHKDTPYTEFHSQSENETQELAMRLILKN
                     WNRLKKEGLLFALTGELGMGKTIFAKGIGQFLKIKAEITSPTYSYLNEYTFQREGCQG
                     TFYHLDAWKIDQQQELALLKIDQLLKANQVMVIEWWDKIAKFLPKSFQQRAIELNLSG
                     QDHWRKIKLWEKNQH"
BASE COUNT         1169 a          801 c          756 g         1485 t
ORIGIN      
        1 aacgataagc attaatttgt gcgttaatta gaaataattc tggtttctta ctgtccttgt
       61 tcaaagcttt gatgtagcta ccagctaagc tagcgtgttt tagtcttaga ctcttaactt
      121 cagctccact taaaacaatc cccgctagat attttttatc gatttggtaa tcaaaataag
      181 cttttttgtt ttttattaac aacatgggct tattgtagca gttttagatt tggatttgat
      241 aaatgaccga gccacttaaa gcaaagcagc tttggccatc gtttgctaga gtaatatcac
      301 tgactccagc taattcattg cttttaattt cactaatcaa ctggccctct ttggtactaa
      361 caatgaggcg attattgctc ttgtctagaa aaacaatttt gtctgaattt tcctgactgg
      421 ctattttaac taaatcagtt gggagttgtt ccaaaccagt taaagcaaaa tccacctgat
      481 aaccgcggct aaatttcata attttgccat ccttaaaacc aagccataag tctccatcga
      541 tactcatgtc actcactgta ctgaagttaa tgttttgttt gtcgactaac cagccgattg
      601 cttcacttaa ctcgccgtta gttaatgtgt agcgataaat attgcgtttt tctgggttaa
      661 gcaaataaag aaaattttgg taactctcta cgaattgggc atctctatca gaatcacctt
      721 cggttttaag ggcgaaacta cttcgatctt gccagtctag atatttaata ccttgatctt
      781 taataaagat ttttaatcga tctgagctgc tgttatcaat actgaaatca gcaaaattaa
      841 ttgtccattg atcaaagttg ttattattta ccagtaatag ccgatcagtt tgactattgc
      901 cgacgagtaa accttgaggg ctggcaatta ttttattgcc taaaaaatct ggatagttgt
      961 cataccagtt gctgtaaaca aacaactgat ctaaattatt ttcactgact aactgttccg
     1021 ctcgagcttg taattcttga attttttctt caattctttt tttagctaat agtgaattat
     1081 attcgtctaa taattgacga agctgttctt gattttcctg aattttttcc ctcgctaaaa
     1141 ctggctgttt agtactgagt tgctctagtt ctgcattact taagactaaa gcatcaattt
     1201 ccttatttgc tttatctagg tttttttgct ctaaatggcg ccagcttaac aaagctgtgg
     1261 tcacaagcac aattgctagt actactaata gaaatatttt ttgatagtgt tttttttgtt
     1321 cttgtcttag gttttttatt tttaaaacaa attctttaat tttttgagga atttttttaa
     1381 tttttaataa aattattttt tgtttagcta atttttcctt ccaagaaact ttttctttat
     1441 gttgataaga ccaaagtaat agagcggtat taaaattgtt tttaaaataa tcgtccagtt
     1501 tctgttcaat aattaattcg agtggatagt tgcggttttt ttcgacaatg acctttccca
     1561 gttgataatt gcccgcaatc aatacaaatt ggtcattttc gatatattgc ccaacaagca
     1621 tactgatctc gctctggcta ctaatcagtt ctctgagctg ttgatcacgc ttcaacaaaa
     1681 tgctaccacc ctgacaggct aaaataattt ttttaccaac ctttaaacta agagcaaaat
     1741 ttaattgtaa tttttttaac cgacactctt tattaagatc aagcagcgct aaatgtattt
     1801 tttcagcact agtaaattgc caagtactta gccttatttg taaatcttta ccaaaaaatt
     1861 gagcatcagg tccttgaaga cttaaaacca taataaaatt ttcttgacga ttctttaaaa
     1921 aacctacaaa ataattggct tgagatttgg caagtagggg taacaagcta agttcttgca
     1981 ctatcctcat tctaacatgc ttggcaaagc tgcgccgatg gcgagattgg gttggcgatc
     2041 tagttttccc tcaaaggtag ttacccctcg tttcagtgtc aagtgggcag ttagggcgat
     2101 catcgcagca ttatcggcac aaaatttttt gtgataaggc aaatacaaat taattttttt
     2161 agctgacaat ttttggtaat tttttaacgt gtgtctaata ctgttgcgca aggcaatatt
     2221 ggccgccacg ccaccgccta accacaattc attaatagtg gtgattggtg gtttaatttt
     2281 cccttgttta atttttttgc ttaattgagc tttgggataa attttttggt ttaaatcggg
     2341 actgaacaaa attttttcca gtttatagtt aatttgagca aagacctgtt gttggacaat
     2401 ggctgctaaa tcacacaccg cttgagcatc taatttttcc tcttttttaa gttgttttat
     2461 cagttgatga gtggctgttt taaaaccaga aaaactcaaa ttaaaatttt ccacattggt
     2521 catagctaag ggtaaagacc aacgcttggc attacctttt ttagctaatt tttctagaat
     2581 tggtccagct ggatagccta aatcgagcat ccgaccaatt ttatccaaac attcaccagc
     2641 gctatcatca actgtccagc ctagtcgctc ataatctccg ctttttttga tcaagataaa
     2701 ctcactatgt cctccagaaa tgaccatagc tagtactggg aagttaattg ttctactaag
     2761 gttttgggtt ttaggttgaa gcaaggggga cagcgcatga gcttcaatat ggttaactgg
     2821 aataattggc aattgccaca ttttggctaa ttttttggca tagttaatac cgacttccaa
     2881 agccggtgcc aaccccggcc cgacggtgac tgccaccgct tgtagctggt ctttgatttt
     2941 gctctcatca ttaaaacctg ctctatgtaa cgctgtcttg gtacatgcct caatgtttgc
     3001 ttggtgagct tgtttggcta aagtaggaaa aaccccgcca tactgctgat gaatattggt
     3061 ctgactcgcc accacatttg ccagtacccg cgcgccttct aaaatggcta cgctcgtatc
     3121 atcacaactg gtatctatcg ccaaaatctt cttctttttg gctaatgttg gtttttttcc
     3181 cataatttaa ttttgcgcca atgatcttga ccactgagat tgagttcaat ggctcgttgc
     3241 tgaaaacttt ttggtaaaaa tttagcgatt ttatcccacc attcaattac catcacctga
     3301 ttggctttta ataattgatc gattttgagt aaggccaatt cttgttgttg atcaattttc
     3361 caggcgtcaa ggtgataaaa agttccttgg caaccttctc tttgaaaagt atattcattg
     3421 aggtaactat aagttggcga ggtaatttct gctttaattt tcaaaaattg accaattcct
     3481 ttggcaaaaa ttgttttacc catgcccaat tcaccagtca gcgcaaataa taaaccttct
     3541 tttttcagcc gattccaatt tttcagaatg aggcgcattg ctaactcttg agtttcgttt
     3601 tcactttgag aatgaaactc agtataggga gtgtccttat gcaattggcc ccgcagtgcc
     3661 agaggagtag acagggtggt gtcgatcacc actgacggtt catttttggg cagtcgtccg
     3721 acatcaataa ttaaatcaat taattgttgt tgtttctcac ttaagttgtc taaaatatca
     3781 ctaatttggt aggggcgctt ttgattggaa gcattggcgc tagtagcagt gatcccatga
     3841 cctaatgttg caataagctt gaggatcaac ggataatctg gaatccggat ccctaaagtg
     3901 ccaaattcac tagctacccg cttatcgatg tcgacagtct tttttaactg ggaaataaca
     3961 gtatatggtc ctggtaaaaa acgttgataa aaattttggg cttgttgatt gatcttgacg
     4021 tattgttcag ccatggtttg gctgtttact gcgattgaca gcggtttacc ttcacggcga
     4081 cttttgtatt gcaataattt atcaatagct ggtgtgctgt gaggattagc cccgatgcca
     4141 taagtagttt cagttggata aataactaga ccaccgtttt taataacggc gatagcttgg
     4201 tcgatgagcc g
//
LOCUS       DAOSEW010000061         4009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_20439_length_4009_cov_4.424127, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000061 DAOSEW010000000
VERSION     DAOSEW010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20439_length_4009_cov_4.424127"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2588
                     /gene="rpoC"
                     /locus_tag="PLJ02_02750"
     CDS             <1..2588
                     /gene="rpoC"
                     /locus_tag="PLJ02_02750"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOI05358.1"
                     /translation="LGAPEIILRNEKRMLQEAVDSLIDGSQAKQSRRRSTRRPLRSLS
                     EMLRGKTGRFRQNLLGKRVDYSGRSVIIVGPELKLNQCGLPKDMALEMFKPYVLRELI
                     VHGIAPNVKSARHLVDRREAEVYAILEKVIQNHPVLLNRAPTLHKLGIQAFYPVLIEG
                     SAISLHPCVCAGYNADFDGDQMAVHVPLTKEAQQEAIELMMADHNLLKPANGEPITLP
                     NKEMALGVYYYTSFDERLETFPAILGSKNEAYHLYQIGRLKLRQKIQVKMEFDGARQL
                     VETSVGRLMFNSVLPASLRFINQPVEAKKIKQILTKAIETEPETVVAKMIDDIKDLGF
                     EAATKSGLSVGVTDCGMIDEKDQIIAKANDDVEKIQEYYLQGLITDDERRRQSLDVWI
                     KTTEELAEKTWATYDDENSVKTIIDSGGTRASKDQVKQLAAMRGLVVDPLGNIVEMPT
                     KSNFRQGLSIFEYVTSARGSRKGLTDSAIKTADAGYLTRRLVDVAHDVIIRSDDCGTT
                     EGIEIKRLPRKDVFTSRILGRYATQDIKDGKKVLVKAGELITPDLAEAVNQSKVESAW
                     VRSALTCQAKHGLCAKCYGWDFSNRSQVKIGVPVGVLAAQSIGEPGTQLTMRVKHSGG
                     IIGLDVTQGLPRVEELLEARNPKVQAIIAHFPGKVRVKQQANEQEITIKGNNGEEESY
                     TLSSSIKLQVSDKDLVHTGQALTVGGINVQELLQLSGLLATQQYIVDQVQAVYESQGI
                     TINDKHFEVIVRKMGDKLQIDMPGDTDFLVGEVVERSRFVDVNEDVMAAGGDPATARV
                     LLLGITRSSLFTDSWLSAASFQHTKHVLTDAAAEGAIDYLKGLKENVIIGRLIPTSEE
                     RARLE"
     gene            2694..3104
                     /gene="rpsL"
                     /locus_tag="PLJ02_02755"
     CDS             2694..3104
                     /gene="rpsL"
                     /locus_tag="PLJ02_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOI05359.1"
                     /translation="MPTINQLIRKGGRKSRIKRVKATALRRSFNSNTNRPTKTTNPQK
                     RGVCTVVKTMTPKKPNSALRKVARVRLSNKVEVTAYIAGEGHNLAEHSVVLVRGGRVK
                     DLPGVKYHIIRGKYDCAGVDGRKSSRSKYGVKRS"
     gene            3109..3585
                     /gene="rpsG"
                     /locus_tag="PLJ02_02760"
     CDS             3109..3585
                     /gene="rpsG"
                     /locus_tag="PLJ02_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012057171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOI05360.1"
                     /translation="MRTKKANIRQINKDVKYNSTLVQRLINRSMKDGKKSVAQKQVYE
                     ALEIVAKQSKRKPLEALEEAIEKIKPKMEVRSRRVGGASYQVPMPVRDLRAGSLSIRW
                     LVNEANKRPNKQYHSFAEKLAAEILDAMIEQGGAIEKRNTSHRMAEANKAFSHFRW"
     gene            3675..>4009
                     /locus_tag="PLJ02_02765"
     CDS             3675..>4009
                     /locus_tag="PLJ02_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOI05361.1"
                     /translation="MSGISDQREVALDRIRNIGIIAHIDAGKTTTTERILFYTGRNYK
                     TGEVHEGAATMDWMAQEQERGITIMSAATTCFWKPYFNTHLPKERYRFNIIDTPGHVD
                     FTAEVERSLR"
BASE COUNT         1275 a          666 c          883 g         1185 t
ORIGIN      
        1 atctaggtgc tccagaaatt attttacgca atgagaaaag aatgttacaa gaggccgttg
       61 attctttaat tgatggttct caagcaaaac agagtcgtcg tcgttctact cgtcgacctc
      121 tccgctctct atcggaaatg ttgcgaggta aaaccggtcg tttccgccaa aatttgttag
      181 ggaaaagagt cgattactct ggtagaagcg tgattattgt tggtcctgaa ttgaaattga
      241 atcaatgtgg tttaccaaaa gatatggccc tggaaatgtt taaaccttat gttttacgtg
      301 aactaattgt tcatggtatt gcaccgaacg tgaaaagtgc gcgtcactta gttgatcgtc
      361 gtgaagctga ggtttatgca attttggaga aagttattca aaatcatcca gttttattaa
      421 accgtgcccc aactttgcat aagcttggga ttcaagcttt ttatccagtt ttgattgaag
      481 ggagcgccat ttcccttcat ccttgtgttt gtgctggcta taacgccgac tttgatggtg
      541 atcagatggc cgtccatgtg cctttaacta aagaggccca acaagaagcc attgagttga
      601 tgatggctga tcataactta cttaagccag ccaatggtga gcctattact ctgccaaata
      661 aagaaatggc attgggtgtc tactattaca ctagttttga tgagcgcttg gaaactttcc
      721 ctgctatttt gggtagtaaa aatgaagctt atcatcttta tcaaattggt cggttaaagt
      781 tgcgtcaaaa aattcaggta aagatggaat tcgacggtgc acgacaattg gtggaaacca
      841 gtgtaggtcg tttaatgttt aatagtgttt taccagcgag cttgcgcttt attaatcaac
      901 ctgttgaagc caagaaaatt aaacaaattc tcactaaagc gattgaaact gaaccagaaa
      961 cagtagtcgc caaaatgatc gatgacatta aagatttggg ttttgaagca gctaccaaat
     1021 ctggtctgtc agtcggagtg actgattgtg gcatgattga tgaaaaagat caaattatcg
     1081 ctaaagctaa tgatgatgta gaaaaaattc aagaatatta tttgcaaggt ttaattactg
     1141 acgacgaaag acgccgtcaa tctctcgatg tttggattaa aacaactgaa gaattagctg
     1201 aaaaaacttg ggctacctac gatgacgaaa atagtgtcaa aactattatt gattcaggag
     1261 gtactcgggc ttctaaagat caggtgaaac aactagctgc catgcgtggt ttagtggttg
     1321 atcctttagg taacattgtg gaaatgccaa ctaaatctaa ctttagacag ggcttaagta
     1381 tttttgaata tgtaacttca gctcgtggtt ctagaaaagg tttaactgac tcagctatta
     1441 aaacggctga tgctggttat ttaactagac gtttggttga tgtggctcat gatgttatta
     1501 ttcgttctga tgattgtggc accactgaag gtattgaaat taaacgttta ccaagaaaag
     1561 atgtttttac ttcgagaatt ttgggtcgct atgctactca agatatcaaa gatggtaaaa
     1621 aagtgttggt taaagctggt gaattgatta ctccagattt agctgaagca gttaatcaaa
     1681 gcaaggtgga aagtgcttgg gtcagatcgg cactaacatg tcaagctaaa catggtctct
     1741 gtgccaaatg ttatggttgg gatttctcca atcgtagtca ggtcaaaatt ggcgtgccag
     1801 tgggtgtatt agcggcccaa tcaattggtg aaccaggaac tcagctgacc atgcgagtta
     1861 aacactcggg aggtattatt ggtctagacg tgactcaggg tttgcctcgt gttgaggaat
     1921 tattagaagc tagaaatccc aaagtgcagg caattattgc tcatttccca ggaaaagtta
     1981 gagttaaaca acaagccaat gaacaagaaa ttactatcaa aggcaataat ggcgaagaag
     2041 aaagttatac tttgtctagc tcaattaaac ttcaagtcag tgacaaagat ttagttcata
     2101 ctggtcaggc tttaactgta ggtgggatta atgttcaaga attgttacaa ctttctggat
     2161 tattagccac tcaacaatat attgtggatc aagttcaggc tgtttatgaa tcacaaggta
     2221 ttaccatcaa tgataaacat tttgaagtca ttgtccgcaa gatgggtgat aaattacaaa
     2281 ttgatatgcc aggtgatact gatttcttag ttggtgaagt cgttgaacgt agtcgttttg
     2341 ttgatgtcaa tgaggatgtg atggcagccg gaggtgatcc tgccactgcc cgtgtgcttt
     2401 tgcttggtat tactcgatct tctttgttta ctgattcctg gttatcagcc gcttctttcc
     2461 aacacactaa acatgttcta actgatgcgg cggcagaggg agccatcgat tacctaaaag
     2521 gtttgaaaga gaatgtaatc attggtcgtt taattccaac tagtgaagaa agggctcgtt
     2581 tagaatagag attccctaga taaacttgat ggcttgtgtt agaattgcca ttcgatcaat
     2641 aaaagttagc aatgaatgtt attaggatca aaaataaaca aaattaaaag attatgccaa
     2701 ctattaatca actaattaga aaaggtggga gaaaaagtag aattaagcga gttaaggcta
     2761 cagccttacg tcgcagtttt aactccaata ccaatcgacc cactaaaacc actaatcccc
     2821 aaaaacgggg tgtatgtact gtggttaaaa ccatgactcc taagaaacca aactcagctt
     2881 tacgtaaagt agcgagagtg cgtttatcta ataaggttga agttacagct tatattgctg
     2941 gtgaaggaca taatctagct gaacatagtg tagttttggt gcgtggtggc agagtaaaag
     3001 acttgccagg tgtgaaatat cacattatcc gtggtaaata tgattgcgct ggagttgatg
     3061 gacgtaaatc aagtcgcagt aaatatgggg taaagaggag ttaagcttat gagaactaaa
     3121 aaagctaata ttcgtcaaat caacaaagat gttaaataca actcaacttt ggtacagcgt
     3181 ttgattaacc gtagtatgaa agacggtaaa aaatcagttg ctcaaaaaca ggtttatgaa
     3241 gcactagaaa ttgttgctaa acaaagcaaa cgcaaacctt tagaggcatt agaagaagcc
     3301 attgaaaaaa ttaagccgaa aatggaagtt agatctcgtc gggtaggagg agcttcttat
     3361 caagtaccta tgccagttag ggatttaaga gctggatctt taagtattcg ttggctggtt
     3421 aatgaggcca ataaaagacc caacaagcaa tatcatagtt ttgctgaaaa attagcagct
     3481 gagattttag atgcgatgat agaacaagga ggagcgattg aaaaacgtaa cacctctcac
     3541 cgcatggctg aagcaaataa agctttttct cattttcgtt ggtaaattct tgctagtttt
     3601 tagaaaaaaa tataaaatta tttgtttata taaattgtat taattattaa aagtaaatcg
     3661 ttgtaaaagt atatatgtca gggatttcag atcagcgtga agtggcctta gatcgaatta
     3721 gaaatattgg gattattgct catattgacg ctgggaaaac tactactacc gaaagaatac
     3781 tgttttatac tggtcgaaat tataaaaccg gcgaggttca cgaaggtgca gctaccatgg
     3841 actggatggc tcaagaacaa gaacgtggaa ttactatcat gtctgctgca accacctgtt
     3901 tttggaaacc ttattttaac acgcatctac ccaaagaacg ttatcgcttt aatatcattg
     3961 atacaccagg ccacgttgat tttactgccg aggtggagcg ctctcttcg
//
LOCUS       DAOSEW010000062         3997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_20529_length_3997_cov_3.871892, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000062 DAOSEW010000000
VERSION     DAOSEW010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20529_length_3997_cov_3.871892"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..1529)
                     /locus_tag="PLJ02_02770"
     CDS             complement(57..1529)
                     /locus_tag="PLJ02_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05362.1"
                     /translation="MSTNKEQPIESQENSVRKEVNNPYEYYSHPVVTKAIAQFCGAED
                     LPANFSLTDTNNTLNDWLCEYLAVANNQIAKEKSGGSPARSIKNHCLADMLNWQPTSE
                     IFMSFWQKETLNSQEKLLPSRSLFILDSEYYNKQYPERFLVDQLGVFQLMEPVYQIIT
                     EKLRQYGFNYNTVMTGKGYHFLTQISNESPILEKIIDLGQKVDPALKELQATVPSDSK
                     RDRIVPLATQQAHQGMGRLVQYLVSQVINDIRNNSPIPIEISDVGMEGVALDLTPNLV
                     RAVDTGVIGIPGSIYLKPQFKPDIYNQNGAVDRTRILTRVYRESNGQEIEKLEQLILT
                     RQNFNLAINNLQQANSKIPESELGLAKLIRDYQQSELYQFHQALDKNPGDHWTEWPHT
                     YRNYAGIAGDDPILQRILAPGSHDLLEPGSLNYTLQHLFEQWGGNDDLSVAGHVRTFL
                     RSAYEDPRFNWGNRFTRHYSATQHAEGWATIILGQRFDKK"
     gene            complement(1536..2522)
                     /locus_tag="PLJ02_02775"
     CDS             complement(1536..2522)
                     /locus_tag="PLJ02_02775"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOI05363.1"
                     /translation="MKPENRLPKVESKEKIETKKTNLGLAAYIEPQEISNQTAHNMAW
                     HQLLNSWRNLDCLEIQYEHDYLTNWWMSLPAMLKDFSSPIIVHGPTKKRDIATTDDQL
                     RARSLAENKRALDLSAAVGAQAMILHLTPQDDFQLRIPQLDRAIASFTELSQYIEENH
                     YPVSLMLENLEYPKWPTDTKEAVDLLKYLKMIHPNLTSCVDLAHLWHNYAALLPGANY
                     VEQDFPDLLIDYIVEVNSIAPIRRFHFAGAYIDKKNNIHETHGYPELNGKFKNSEFLD
                     FMPITPSLKIMDEFIKMQNKNHEPAADIILEMHSNKEKQEQALTTIQNYLKD"
     gene            2599..3315
                     /locus_tag="PLJ02_02780"
     CDS             2599..3315
                     /locus_tag="PLJ02_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097159 - organic cyclic compound binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5-like heme/steroid binding
                     domain-containing protein"
                     /protein_id="HOI05364.1"
                     /translation="MKKIIFIFVFGLFNIFATSNLLNIVQAQSSYSLNEVKLHQTPED
                     CWMAIEGQVYDLSNYLPDHDRYLDIREWCGQEATADYNSKAGRNRAHSPRADELLAEY
                     LIGELVSSEEADLVQTTKVKEAINSPNPDLAPRYQVFLPVLLTIIFYYLTKKILGKAR
                     HDFLWNTVLLIGLIPTVGFGLILALADQIAFLDSVNLAELLHEHAQLSIIIGTAMILH
                     FVKRAKIYLSQGKSSFRKRK"
     gene            complement(3320..3604)
                     /locus_tag="PLJ02_02785"
     CDS             complement(3320..3604)
                     /locus_tag="PLJ02_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05365.1"
                     /translation="MKENFQINNFDQLKSSVTATLELARPREYIGLGNEKIFTPFVIG
                     EKFSKLHPHQTGMDQHKGNMVIIATNIVGSRAIAKCVLPSCHEMILLEAK"
     gene            complement(3617..3859)
                     /locus_tag="PLJ02_02790"
     CDS             complement(3617..3859)
                     /locus_tag="PLJ02_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05366.1"
                     /translation="MDKENLQKIIISAPCENLEDEARLNQTVFAFNQLDNPNAVMGTK
                     EINTGLPYLLEVLVEADSATAFIDYLADAAPEAEIK"
BASE COUNT         1147 a          695 c          757 g         1398 t
ORIGIN      
        1 aattgccttt ttgacaggca gagactctag aattgccttt ttgacaggat gagactctat
       61 tttttatcaa atctttgacc aaggatgata gtggcccaac cctcagcgtg ttgggtggca
      121 ctgtaatgtc tggtgaaacg gttgccccag ttaaatctag gatcttcgta agccgaacgc
      181 aaaaaagttc gcacgtgacc agcaacactc aaatcatcgt taccacccca ttgttcgaat
      241 aaatgctgca gagtgtagtt gagactgccg ggttcgagta aatcatgaga gcctggtgct
      301 aaaattcttt gcaagattgg atcatctcct gcaatccccg cataattgcg ataagtatgt
      361 ggccactcgg tccagtgatc gcccggattt ttatctaaag cttggtgaaa ttggtaaagt
      421 tctgattgtt ggtaatctct aataagtttt gccagaccga gctctgattc tggaatttta
      481 ctatttgctt gttgtaaatt gttgatcgcc aagttgaaat tttgtctggt taagattaat
      541 tgctcaagtt tctcaatttc ttgaccatta ctttcacgat aaactctggt taaaattcgc
      601 gttctgtcca ctgccccatt ttggttgtaa atatctggct tgaattgggg ttttaaataa
      661 attgacccag gtatgccgat taccccagta tcaacggctc tgaccaaatt aggtgttaaa
      721 tccagtgcta ctccttccat accaacatct gatatttcaa tgggaatggg actattgttt
      781 ctgatgtcgt taattacttg tgaaaccaaa tattgaacta gcctgcccat accttggtgt
      841 gcttgttggg tggctaaagg cactatgcga tcccttttag aatcactagg aacggttgct
      901 tgtagttctt ttaaggctgg atctactttt tgaccaagat caataatttt ttctaaaatt
      961 ggactctcat ttgagatctg agttaaaaaa tggtagccct tgccggtcat gacggtgttg
     1021 taattgaatc catattgacg caatttttcc gtaataattt gataaaccgg ttccatcaat
     1081 tgaaaaacac caagctgatc gacaagaaat ctctctggat attgtttatt ataatattca
     1141 ctatctagaa taaaaagtga ccgactaggt aacagctttt cttggctgtt cagagtttct
     1201 ttttgccaga aggacataaa aatttcacta gttggttgcc aatttaacat atcggctagg
     1261 cagtggtttt taatagacct ggccggagaa ccaccgcttt tttccttggc aatttgatta
     1321 ttagcaactg ctaaatattc acacagccaa tcattaaggg tattgttagt gtcggttaag
     1381 ctaaaattgg ctggtaagtc ttctgctccg caaaattggg caatagcttt ggtaacaact
     1441 ggatgagaat aatattcgta agggttattg acttctttac gaactgaatt ttcttgtgat
     1501 tcaattggtt gttctttatt agtagacatt gaaatttaat cttttagata attttgtatt
     1561 gtggttaaag cttgttcttg tttttctttg ttagagtgca tttccaaaat aatgtcggca
     1621 gctggttcgt gatttttgtt ttgcatttta ataaattcat ccataatttt taaacttggc
     1681 gtgataggca taaaatcaag aaactcactg tttttaaatt tgccattcag ttctgggtaa
     1741 ccatgtgttt catggatatt attttttttg tcgatgtaag ctccggcaaa atgaaatcta
     1801 cgaatagggg caatactgtt aacttcgact atgtagtcaa tcaaaaggtc tgggaaatcc
     1861 tgttcaacat aattcgctcc cggcaataaa gctgcataat tatgccataa atgagctaaa
     1921 tcaacacaag aggttaaatt ggggtgaatc atttttaaat attttagtaa atcaacagct
     1981 tctttagtgt cagtcggcca tttggggtat tctagatttt ctaacattag agaaactgga
     2041 taatgatttt cttcaatata ttgtgataat tcggtaaaag aagcaatagc tcgatctaat
     2101 tggggaattc tcaattggaa atcatcttgg ggggttaggt gtaaaatcat ggcttgagca
     2161 cccacagcag ctgataaatc caaagccctt ttgttttctg ctaatgaacg agctcttagt
     2221 tgatcgtctg tggtggcgat atctcttttt ttggtcgggc catgaacaat gattggtgat
     2281 gaaaagtcct ttaacattgc tggtaaactc atccaccagt ttgttaaata atcatgttca
     2341 tactgaattt ctaaacagtc taaatttcgc caactgttta acagttgatg ccaagccatg
     2401 ttgtgagctg tttgattgct tatttcttga ggttctatat aggcggccag tcccaaattc
     2461 gttttttttg tttcaatttt ttctttgctt tcaacttttg gtagcctatt ttcaggtttc
     2521 atactggaaa attatactat gagttcagtg taaataaaaa ttaaaattaa cccactttta
     2581 tagtatagta aggtgtatat gaaaaaaatt atttttatct tcgtctttgg actttttaat
     2641 atttttgcta ctagcaattt gctaaatatt gttcaggccc aaagttcata ttcattgaat
     2701 gaggttaagt tgcatcagac tccagaggac tgttggatgg caattgaggg gcaagtttat
     2761 gatttaagca attatttacc agaccacgat cgctatttgg atattagaga gtggtgcggt
     2821 caagaggcaa ctgcagatta caacagtaaa gccggaagaa atcgagctca tagtcctaga
     2881 gcagatgaat tattagcaga atatttaatt ggcgaattag ttagcagtga agaagcggat
     2941 ttagttcaga ctactaaagt taaggaagca ataaacagtc ccaatccaga cctggcgcct
     3001 cgctatcaag tttttttacc ggttttgtta acaattattt tttattattt aactaagaaa
     3061 attttaggta aagctcgtca tgatttttta tggaacactg tactgttaat tggtttaata
     3121 ccaacagttg gttttggttt aattttagct ctcgctgacc aaattgcctt tttggatagc
     3181 gttaatttgg cagaattact tcacgagcac gcccaactat caattattat tggcacggca
     3241 atgatcttgc acttcgttaa acgcgcaaaa atttatttat ctcaaggaaa atcgtctttc
     3301 agaaaacgaa aatagcgatt tattttgctt ccagtaagat catttcgtgg caactaggta
     3361 acacacattt agctatagct cgactgccaa cgatattagt agcaatgata accatgtttc
     3421 ctttatgttg gtccattcca gtttgatgcg gatgtaattt agaaaatttt tcaccaatca
     3481 caaaaggtgt aaagattttt tcattgccaa gtccaatata ttcccttggt cttgctaatt
     3541 ctaaagttgc cgtaacacta ctttttaact ggtcaaagtt attaatttga aagttttcct
     3601 tcatcagaga ctttttttat ttaatttctg cttctggagc tgcatcagct aaataatcaa
     3661 taaaagccgt agcactgtca gcttcgacta aaacttctaa aagatatgga agaccagtat
     3721 ttatctcttt ggtccccatt accgcattcg gattatcgag ttgattaaaa gcaaaaactg
     3781 tttgattcaa acgagcctca tcttcaagat tttcgcaggg agcgctaatg ataatttttt
     3841 gtaaattttc cttgtccata agacctttct taacatactt ttaatgtatg caagtatatc
     3901 atactaatag ccaatttatt gccaaaaaat gctttggatg gtcgatagct ttatttttca
     3961 ggacttatat tgactgtttc ttttagtttt tggatta
//
LOCUS       DAOSEW010000063         3994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_20555_length_3994_cov_4.359482, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000063 DAOSEW010000000
VERSION     DAOSEW010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20555_length_3994_cov_4.359482"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..546)
                     /locus_tag="PLJ02_02795"
     CDS             complement(103..546)
                     /locus_tag="PLJ02_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05367.1"
                     /translation="MIYLNYFLQLVFILFLLSVESTLATPIFSLFLAFKFLDRFNPRD
                     DHSFYWLLVLLLLISLAVALFYQLGVGVSVAMIAVYYYLRTLLGRKVFLKSSRQWQLL
                     QLLLFAVLQVTIFFLSDLTVNYFMILQGVVILIFLIYKTISINKL"
     gene            complement(543..1310)
                     /gene="mreC"
                     /locus_tag="PLJ02_02800"
     CDS             complement(543..1310)
                     /gene="mreC"
                     /locus_tag="PLJ02_02800"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOI05368.1"
                     /translation="MFNYQKVGRYFLIIVVLVFIFLLDFLGLLALPNLRRLAINWQAA
                     NYRAISTLVRPVTQLKEFWQLRQRLEDLEYRYREASVQLTELAALREENQALRALLEN
                     SDRALSKNLITKPISSFAKPVLAIGSDSGLQVGSLVLGEGNLLGIVEEVSQQQARVLL
                     LKNMLEQGIVAKTESGVMGIVKGDGRNILLTEVASEETLNLRERVVTVGQEGIPPDIF
                     IGRIISLKEINPAKSTQEAILQQDVNFYELSLVEVQP"
     gene            complement(1303..2019)
                     /locus_tag="PLJ02_02805"
     CDS             complement(1303..2019)
                     /locus_tag="PLJ02_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05369.1"
                     /translation="MEKGIELIDLSYQPWVFLWDANQEQIINFGQEALDWWQLYRRDA
                     GNEHWQLVYPLKSGILQSQWALEQVLQHDFVLNREHLKKAKRLQIVVTGANTNLEQET
                     IRRSFAQNFLKPVVIVERAAFLNQYLRAKQDFSKLKLIIDLNLDFAELSLFLLDKNLK
                     SKHLPLSENLDEAMLIKRLKAAIDSFILNVPSKLFQEKWQYFYLFVHPQLKSSSILDE
                     LSRHLKMEGILNQEQLTAYV"
     gene            complement(2041..3123)
                     /locus_tag="PLJ02_02810"
     CDS             complement(2041..3123)
                     /locus_tag="PLJ02_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05370.1"
                     /translation="MTNIQDDQNVQKQPTKADVDELLNQLEALSQEIAAKTVVDQPQL
                     EAAPTPPALAGSDEPQLPASQQTDGQKKDLDLDKFISELETKIAEKDEGTTPGEAAPQ
                     SQTQPIEPWPEPAKPQSEPAEPQLEPAESPVQPVTEPAIKEEQSNFPDNFSRQRPSLD
                     LSEVEEKEAPSLTLNNGRLEPTPEPTAEPSAEPTLEPTPKPTAEPSAEPTPEPVNEED
                     LEKQNIFAMLGLKNLEESEKEAFLADLEQLIWDNFVEVELPLLLNSEQKAQADQILAD
                     QSKAENERREALLAYVEPFIPNLDEVMYKKALSLKKEMFEERVKKMRDQATEENNLSL
                     MADLDQIQELIAAGRYKTATELLNKA"
     gene            3207..>3994
                     /locus_tag="PLJ02_02815"
     CDS             3207..>3994
                     /locus_tag="PLJ02_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05371.1"
                     /translation="MTSQRQKIDDFLQKLIFALFNILVFATPFVFTWFNQELFEFNKM
                     IVVYLFTSLILAVWAGRSLIRKQLLWKNRTHNYLVALFFFSQLLSTIFSMHQRTSIFG
                     YYTRFNGGLLSIIAYLALFFAAQYNLSKKQLNLILKNLLFAALLISLYAIPEHFGHSL
                     SCLIVTGKYNVACWVQDVQNRVFATFGQPNWLAGFLLMTIPLAIWQIWETMHNQGKKK
                     TRWPIIFNLLLLFLLVTALFFTKSRSGLLALGVALLVQIIAEKF"
BASE COUNT         1199 a          815 c          664 g         1316 t
ORIGIN      
        1 gcaaatcaat taagcgcaaa cccaaaaaag taaaaagaaa aaacaaaaag atcatcagcc
       61 aaaacaactt cttttgatct tgataagcaa aagtgttttt tttcataact tattaatgct
      121 aatcgtttta taaattaaga aaattaaaat caccacgcct tgtaaaatca taaaataatt
      181 aacagtcaaa tcagacaaaa agaagatcgt cacttgtaag acagcaaata gtaatagttg
      241 caaaagttgc cactgccgag aacttttaag aaaaactttt ctccccaata aagttcttaa
      301 ataatagtag acagcaatca tcgccacact aacgccaaca cccaattgat aaaataaggc
      361 aactgctaaa ctaatcaata ataacagcac gagtagccaa taaaaactgt ggtcatctcg
      421 cggattgaac cgatccaaaa acttaaaagc caaaaataag ctaaaaatcg gggtcgccaa
      481 agttgattcc acactcagta aaaataagat aaaaaccaat tgcaaaaaat agtttaaata
      541 aatcatggct gcacctcgac taaacttaat tcataaaaat taacatcttg ttgcaaaata
      601 gcttcctgcg tcgacttagc tggattaatt tcctttaaac taatgattct gccaataaaa
      661 atgtcaggag gaatcccttc ctgtccaact gtgactactc gttctcgtaa atttaaagtt
      721 tcttcactgg caacttcagt taacaaaata tttctgccat ctcctttaac aatacccatc
      781 accccagatt ctgttttggc cactatgcct tgttcaagca tatttttcag caataggact
      841 cgagcttgct gttgactaac ttcttcaact atacctaata aattaccctc ccccaaaacc
      901 agcgatccaa cttgcaaacc gctatcacta ccaatagcca atactggttt ggcaaaggaa
      961 ctaatcggct tggtaattaa atttttgctt aaagcacgat cactattttc caaaagtgct
     1021 cgcaaagctt gattctcttc gcgtagcgca gccaattcag ttaattgcac tgaagcttct
     1081 cgataacgat attcgaggtc ttctaatctt tgcctcaatt gccagaactc ttttaattga
     1141 gttactggtc gcactaaagt agaaatagct cgataattgg ccgcttgcca attaattgcc
     1201 agtcgacgca aatttggcaa agccaataat cctaaaaaat ccagcaaaaa gataaatacc
     1261 aacacaacaa taataagaaa atagcgacca actttttgat aattaaacat aggcagtgag
     1321 ctgttcctga ttcaaaatac cctccatctt taagtgacga cttaattcat ccaaaattga
     1381 ggaacttttg agctggggat gaacaaaaag gtaaaaatat tgccattttt cttgaaaaag
     1441 tttgctgggc acattaagaa taaaactatc tattgcggct ttcagacgtt taattaacat
     1501 tgcttcatca agattttcac ttaatggcag gtgcttactt tttaagtttt tatctaataa
     1561 aaacagagat aattccgcaa agtctaaatt caaatcaata attaatttta attttgaaaa
     1621 atcttgttta gcgcgtaaat attggttcaa aaaggcggct cgctcaacta taactactgg
     1681 ttttaaaaaa ttctgagcaa aactacgtct aatcgtctct tgttctaaat tggtattcgc
     1741 ccccgttaca actatttgta aacgtttagc tttttttaag tgttcacgat ttagtacaaa
     1801 atcatgctgt aaaacttgtt ccagtgccca ctgcgattgt aaaatgcccg acttcaaggg
     1861 ataaaccaac tgccaatgtt cattgccagc gtcccgtcga tacaattgcc accaatccaa
     1921 ggcttcctga ccaaaattaa taatttgttc ttgattggca tcccacaaaa aaacccaagg
     1981 ttggtagcta agatcaatta gctcaatccc tttctccata tatcaaagct taacagaaac
     2041 ttaggcttta ttcaaaagtt ccgtggcggt cttgtagcga ccggctgcta ttaactcttg
     2101 gatctggtct aaatcagcca tgaggcttaa attattttct tcagtggcct gatctctcat
     2161 cttttttact ctctcttcaa acatctcttt tttcaacgat aaagcctttt tatacattac
     2221 ttcatcaaga ttgggtataa aaggttcaac ataggccaaa agagcttctc tacgctcatt
     2281 ttctgcctta ctttgatcag ctaaaatttg atcagcttgg gctttttgct cgctgtttaa
     2341 aagtaatggt aattctactt ctacaaaatt atcccaaatc aattgttcta aatcagctaa
     2401 aaaagcttct ttttctgatt cttccagatt ttttagaccc aacatagcaa agatattttg
     2461 tttctctaaa tcttcttcgt taactggctc aggagttggc tctgcacttg gttcagccgt
     2521 tggctttgga gttggctcta gagttggctc cgcacttggt tcagccgttg gctctggagt
     2581 tggctctaat cgtccattat ttaatgtcag actgggtgct tctttttcct cgacttctga
     2641 taaatccaaa gagggtcgtt gacgagaaaa attatctggg aaattggact gttcttcttt
     2701 tatagcgggt tctgtgactg gctggactgg tgattcggct ggctctagtt gcggttcagc
     2761 tggctccgat tgcggtttgg ctggctctgg ccacggttcg attggttggg tttgactttg
     2821 aggcgctgct tctccaggag tagtaccctc atctttttcg gcaattttgg tttctaattc
     2881 actgataaac ttgtccaaat ctaaatcttt tttttggcca tccgtttgct ggcttgctgg
     2941 taattgaggc tcatcactac ctgccaaagc tggaggcgtt ggagcagctt ctaattgagg
     3001 ctgatcaaca acagttttag ccgcaatttc ttgactaaga gcttcaagtt gattaagtaa
     3061 ttcatcaaca tccgctttag ttggctgttt ttgtacgttt tgatcgtctt gaatattggt
     3121 cataggcagg ctttcttatt tactccttta atttaacaca aattaagagt aaaaagaagc
     3181 agaaaatccg ctatactatg cccataatga ctagtcaaag acaaaaaatt gacgattttt
     3241 tgcaaaagtt aatttttgct ctttttaata ttttggtctt tgctaccccc tttgttttca
     3301 cttggtttaa tcaagaattg tttgaattta ataaaatgat tgtggtttat ctgttcacta
     3361 gtttaattct agcggtttgg gctgggcgct cattaattag gaaacaactc ttatggaaaa
     3421 atagaaccca taattacctt gttgctttgt ttttcttttc tcagttactg tcaactattt
     3481 tttccatgca tcagcggacc tcaatttttg gttattacac gcgttttaat ggcggtttgt
     3541 tatcaattat tgcttatctt gccttatttt ttgctgctca atataacctt agtaaaaaac
     3601 aactaaattt aatcttaaaa aatttacttt ttgccgctct tctaattagt ttgtatgcca
     3661 ttcccgaaca ttttgggcac tcactaagct gtttaatagt cactggcaaa tataacgtgg
     3721 cttgctgggt tcaagatgtg caaaatcgcg tttttgccac ttttggccaa cccaactggt
     3781 tggctggttt cttgttgatg actattcccc tagccatttg gcaaatctgg gaaactatgc
     3841 acaaccaagg caaaaagaaa acccgctggc caattatttt taatctacta ttgctatttt
     3901 tattagtgac tgctctcttt ttcaccaaat ccagatctgg tcttttggct ctgggcgttg
     3961 ctttattggt gcaaatcatc gctgagaaat tttt
//
LOCUS       DAOSEW010000064         3900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_21293_length_3900_cov_2.659818, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000064 DAOSEW010000000
VERSION     DAOSEW010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21293_length_3900_cov_2.659818"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="PLJ02_02820"
     CDS             <1..832
                     /locus_tag="PLJ02_02820"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOI05372.1"
                     /translation="YLHFPDQFRPEDLLQLRSWTKYSVARAKKVLTISNFSKSEIIKY
                     YGRRAEDIIVAPPATDFGKSASQQESKQFFKDHGLKAGYFLYLGTLQPRKNLQSLLKA
                     YDLFLMQLLEQTSTGKDKALAKAPQLVIAGKIGWLNEQFLANINQFIFKEKIILTGFV
                     PAKFKKALYQRAKLSFLLSLYEGFGIPPLESLEAGCLPIVANNSSLTEVVGDPQLTVD
                     PQDIAAIAQKMLEFSQLSESDYQKLLKQRQQHLKQFSWRKSARIILRQLKQIAEEAHD
                     "
     gene            825..1325
                     /locus_tag="PLJ02_02825"
     CDS             825..1325
                     /locus_tag="PLJ02_02825"
                     /inference="COORDINATES: protein motif:HMM:NF014166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOI05373.1"
                     /translation="MTSPIIIGIDPGYDRLGWAVGQSGPNGWQILDQGCIITNRQGLI
                     EERYQQILTDLAAIIERYHPEEAAIETLFWFKNQTTAMQVSEARGLIMATLLRAGLKI
                     SQYTPLQIKQAVTGYGRADKKAVEKMVRLELAIDQNRAKDILDDTFDALALLLCHQAS
                     RKLKQL"
     gene            1322..1909
                     /gene="ruvA"
                     /locus_tag="PLJ02_02830"
     CDS             1322..1909
                     /gene="ruvA"
                     /locus_tag="PLJ02_02830"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOI05374.1"
                     /translation="MIAYLKGPVMVKDHYLIICCGGVGYQVYAPSKFLHSAVAAEIAG
                     DELEAHIYTHVREDKLELYGFAQEQDLALFQLVLTVSGVGPKTALAIVDGGADQLITA
                     VQEANLSFFTAVPRVGKKLAQKIVIELKSKLGSLKELNLATLSPKRQDVYDALLGLGF
                     ADGEINLALEKVDVDNLDLAEAIKQALKIAGGKKI"
     gene            1906..2919
                     /gene="ruvB"
                     /locus_tag="PLJ02_02835"
     CDS             1906..2919
                     /gene="ruvB"
                     /locus_tag="PLJ02_02835"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009004308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOI05375.1"
                     /translation="MKPSPIDPHLNNNDEEELLFTSLRASNWDEFLGQDNIKKSLKIA
                     IDAARQRQEAVDHILFYGPAGLGKTTLAQLIAKEMGVNFHITSGTALSKTSDLAAILT
                     NLEDGDVLFIDEIHRLPKAVEETLYPAMEDFALDIVIGKGPSARTVRLDLAKFTLVGA
                     TTRFGLLTGPFRDRFGLIHRLEFYKPEELEKIIEYASKKLHCQIDQPARLSLAKRARG
                     TPRIALKLLKRVRDFAQVLEKDQIDEALVNQALEIFEIDQLGLDENDRRLLMAMINKH
                     DGKAVGLKTLSASISEDAGTVEEVLEPYLLQIGFIRRTPKGRIITNKAYRYFGITPPE
                     SNQ"
     gene            2971..3594
                     /locus_tag="PLJ02_02840"
     CDS             2971..3594
                     /locus_tag="PLJ02_02840"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOI05376.1"
                     /translation="MPITDKNGQVLSTRQVLHKGKTRFKTILLELWTGFLWWGVGFLP
                     FHSLRRFFYRLSGMKIGPQSTIHMMARIYQPAGISIGQGTLIGEKVTLDGRRQLPHSH
                     GGLSIGNHVDIASEVMIWTSEHDVKSSDMTTIEERVVIGDYVFIGPRAIIMPGVIIGE
                     GAVVAAGAIVTKDVAPYQIVAGVPAKVIGQRPTTATNYRLGRPRLFQ"
     gene            3607..>3900
                     /locus_tag="PLJ02_02845"
     CDS             3607..>3900
                     /locus_tag="PLJ02_02845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOI05377.1"
                     /translation="MPKLLSAIIVSYNTKDLTIQALTSLLKEIKSSDLLKKKSEILLV
                     DNHSQDGSIQAIKQFFHQAKFRDYQLIVNKKNLGFARANNQALKQAQGQYLLLL"
BASE COUNT         1129 a          838 c          864 g         1069 t
ORIGIN      
        1 atatttacat tttcctgatc aatttcggcc tgaggactta ctacaactgc gctcgtggac
       61 caaatatagc gttgctcgag ccaaaaaagt gctaaccatc agcaattttt ccaaaagtga
      121 aatcatcaaa tattatggcc gtcgggcgga agacattatt gtcgctccac cagcgactga
      181 ttttggcaaa agtgctagtc aacaagaaag caaacaattt tttaaagatc atgggctgaa
      241 agctggctac tttttatatt taggcactct gcaaccacgc aaaaatctgc aatccttgct
      301 caaagcttat gatctgtttt tgatgcaact attagagcaa accagtactg gcaaagacaa
      361 ggcgttagcc aaggccccac aactggtaat tgcgggcaaa attggctggc tgaatgagca
      421 atttttagcc aacattaatc agtttatttt taaagaaaaa attattttga ccggctttgt
      481 gccagctaaa tttaaaaaag cactctatca aagagccaaa ttaagtttct tgctctctct
      541 ttatgagggc tttggtattc cgccactgga atcactggaa gctggctgtt tgcccattgt
      601 cgctaataat tccagcttga ctgaggtagt cggtgacccg caattaaccg ttgatccgca
      661 ggatattgca gctattgccc aaaaaatgct cgagtttagc caattatccg agagtgatta
      721 tcaaaaacta ctcaaacagc gccaacaaca cctgaaacaa tttagctggc gcaaatctgc
      781 tcgcatcatt ctgcgccaat taaaacagat cgctgaggag gcacatgact agtcccatta
      841 ttattggcat tgatcccggc tacgatcgcc tcggttgggc agtggggcaa tctggcccca
      901 atggttggca gattcttgac caaggctgta ttattaccaa tcgccaaggc ctgattgagg
      961 aacgttatca acaaatttta actgatttag cggccattat tgagcgttac cacccagagg
     1021 aagccgccat tgaaacttta ttttggttta aaaatcaaac gactgccatg caagtcagtg
     1081 aagcgcgggg cttaattatg gccaccttac tgcgcgctgg cttaaaaatt agtcaatata
     1141 cccccctgca aatcaagcaa gcggtaactg gctatggacg agccgataaa aaagcggtag
     1201 aaaagatggt aagattagag ttagcaattg atcaaaaccg cgcaaaagat atccttgatg
     1261 atacctttga cgcgctggct ttgctccttt gccatcaggc tagcagaaag ctaaaacaat
     1321 tatgattgcc tacctcaaag gaccggtcat ggttaaagat cattacttga tcatttgctg
     1381 tggtggagtg gggtatcagg tttatgcccc gagtaagttt ttgcacagcg cagtcgctgc
     1441 tgaaatcgct ggcgacgagc tagaagcgca catttacacc cacgttcgag aagataaatt
     1501 agagctgtat ggctttgccc aagaacaaga tttagcgctt tttcaattgg ttttaactgt
     1561 ctccggtgtc gggccaaaaa cggccttggc gattgttgat ggtggagcag atcaactaat
     1621 tacggcggtg caagaagcta atttgagctt ttttacggcc gtgccccgag tgggtaagaa
     1681 gttagcccaa aaaattgtca ttgaattaaa gagcaagctc ggtagcctca aagaattaaa
     1741 tctagccacc ctgtcgccca aaaggcagga tgtttatgat gcgctactcg gtttgggctt
     1801 tgccgatggt gagatcaatt tagctttgga aaaagtggat gtggataatt tagacttagc
     1861 cgaagccatt aaacaagcgc tgaaaatcgc tggtgggaaa aaaatatgaa accaagtcca
     1921 attgatccac acttgaataa caatgatgaa gaagaattac tctttacttc cctgcgggct
     1981 agtaattggg atgagttttt gggccaagac aatatcaaaa aatcactgaa aattgccatt
     2041 gatgccgccc gccagcgcca agaagccgtc gatcatattt tgttttacgg gccggccggt
     2101 ttgggtaaaa ctactttagc gcaattgatc gctaaagaaa tgggtgttaa tttccatatt
     2161 acttcaggta ccgccctctc caaaaccagc gatttagcgg ccattttaac caatttggaa
     2221 gatggtgatg tcttatttat tgatgaaatt caccgcttac ccaaagccgt tgaagaaacg
     2281 ctttacccag cgatggaaga ctttgctctg gatattgtaa ttggcaaggg ccccagcgcc
     2341 cgcaccgttc gcctcgattt ggccaaattc accttggtcg gggccaccac ccggtttggt
     2401 ttgctcactg ggccattccg tgatcgtttt ggtttgattc atcgcttaga attttacaaa
     2461 ccagaggaat tagaaaaaat tattgaatac gccagtaaaa aactccattg tcagattgat
     2521 caaccagcgc gcttatcact agccaaacgc gcccggggca caccgcggat tgccttgaaa
     2581 ctgttaaagc gtgtccgtga tttcgctcaa gtcttagaaa aagatcagat tgatgaagct
     2641 ttagtcaatc aagccctgga aattttcgaa attgatcaat taggtttgga tgaaaatgat
     2701 cgccgtttat taatggccat gatcaacaaa cacgatggca aagccgtcgg tctcaaaacc
     2761 ctttcggcca gtattagtga ggatgctggc acggttgaag aggtgctaga accctattta
     2821 ctacaaattg gctttattcg ccgtactccc aagggtcgca tcattaccaa caaagcttat
     2881 cgctattttg gcatcacccc tcccgagtct aatcaataac tgttaccgat caagctcaac
     2941 agtttgcctt gtctgttgta tactagtcat atgccaataa cagataaaaa tggacaagtc
     3001 ttatctactc gtcaagtact gcacaagggt aaaactaggt ttaagaccat tcttttggag
     3061 ttgtggacag gttttctctg gtggggagta ggttttttac cttttcacag tttacggcgc
     3121 tttttctatc gtttatctgg catgaaaatc ggccctcaat cgaccattca catgatggcc
     3181 aggatttatc agccggctgg gattagtatc ggtcaaggga cattaattgg cgaaaaagtc
     3241 accctcgatg gtcgccgcca attgcctcat tctcatggtg ggttaagtat tggtaatcac
     3301 gtcgatattg cttcagaagt aatgatttgg actagcgaac atgatgttaa atctagcgac
     3361 atgaccacca ttgaagaacg agtagtgatt ggtgattatg tctttattgg cccccgggcg
     3421 attattatgc cgggagtcat cattggtgaa ggggcagtgg tggcagctgg agcgattgtg
     3481 actaaagatg tcgcacctta tcaaattgtg gctggtgtac cagctaaagt catcggtcaa
     3541 cgcccgacaa cggcaacaaa ttatcgttta ggccgtcctc gtttgtttca ataaacttgg
     3601 aaacttatgc caaaactttt atcagcgatt attgtttcct acaacaccaa agatttaacc
     3661 attcaggcac taacgagtct gctcaaagag atcaaaagca gtgatttgct gaaaaaaaag
     3721 agtgaaattt tgctggttga taatcatagc caagatggca gtattcaggc gattaaacaa
     3781 ttttttcatc aagctaaatt ccgcgactac caattaattg ttaacaaaaa aaacctcggt
     3841 tttgcgcggg cgaataacca agcgctgaaa caagctcagg gccaatattt gttactgtta
//
LOCUS       DAOSEW010000065         3810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_22078_length_3810_cov_4.214913, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000065 DAOSEW010000000
VERSION     DAOSEW010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22078_length_3810_cov_4.214913"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PLJ02_02850"
     CDS             complement(<1..332)
                     /locus_tag="PLJ02_02850"
                     /inference="COORDINATES: protein motif:HMM:NF020892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOI05378.1"
                     /translation="MSKTFYVTTTLPYVNSSPHIGFALEIVEADVIARYHREILNEQV
                     IFNTGTDEHGQKIADQAKAKGLDPQDYCDHFAQQFQNLKNELNLSNTHFIRTTNPKHS
                     AAAQEFWRR"
     gene            complement(344..772)
                     /locus_tag="PLJ02_02855"
     CDS             complement(344..772)
                     /locus_tag="PLJ02_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05379.1"
                     /translation="METTQTQKKLRGHSRLKTQAIPVDRRKIRKAKNSNSLPDLILAL
                     VCFLAWQYLMFYTSPNDVANLLYANSYLPFVILIFFTVFFFLKFALKKVRLAFCLAFE
                     LLIMLVFHLQNLDFNYYWIIILAIPPFIWLIVNQIEKRLL"
     gene            795..1934
                     /gene="rmuC"
                     /locus_tag="PLJ02_02860"
     CDS             795..1934
                     /gene="rmuC"
                     /locus_tag="PLJ02_02860"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOI05380.1"
                     /translation="MNLVIIISLAILILLVSFLLFFLLKKSQKSIEDEKILDEKLEST
                     VNKVFGMTANKIAYQSKNILQSEQDLLKTDLANKQATIEKLVKQLQEDLEQRQKEIRE
                     IEKQQIDKIGRVATAIEDHRVLTKDLKVSTEKLAKILDNNQTRGAWGERIIEDLLQAN
                     GLQEGVHYVRQTKQSSSNLIPDITLLLPDKRNVAVDVKFPYQEIQKMANTDDKTEKKN
                     HLQQFARDLRLKVEKVAEYISPESDTLDYAILFVPNEMIFSFINQKLPDIIDEALAKK
                     VILCSPFSFLVVARTVMESYRNFMVGKKIQEVIKQVEAFSGEWVKFDEEFQKFGRSIE
                     SLQKNFDTLSTTRKKQMEKRIHKIEEYRSGSGQLLADGHKSGGPH"
     gene            2089..3519
                     /locus_tag="PLJ02_02865"
     CDS             2089..3519
                     /locus_tag="PLJ02_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009150647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOI05381.1"
                     /translation="MSKLNLSRTTVIDSKNKVSETVKLAGWVANKRDHGKVTFIDLRD
                     ETGLIQCVGINDKLKDLTIESVVEVIGQINKRPAKLVNQEMATGEIEVFVEDYQILNK
                     SIELPISINSSGLEINEELRLQYRYLDLRRQRLQKNLRLRSKLINSFRQELIDQDFVE
                     IETPMLTKSTKEGARDFIVPSRFYPGKFYALPQSPQQYKQLLMAAGFEKYFQFARCIR
                     DEDLRADRGFEFTQLDIELSFIDQPTIMQLLENLVKKVCQQLNVQIKDQQFPVVTYQE
                     AMEKYGADRFDLRTAEDKERGVLAFAWVVNFPFFKKVNKADRAEVLDSKSNWTFTHNP
                     FSNPIPEHLDNHLAGKNVEQIIASQYDLVCNGYEVGGGSLRAHRPEVLRATFKIMGYS
                     DEEIEESVGHMLKAFKLGTPPHGGIAFGIDRLLMLITGEDNLRETMAFPMTASGRTSV
                     MDAPSSVSKEQLAELKLSVTADKKSS"
     gene            3529..>3810
                     /locus_tag="PLJ02_02870"
     CDS             3529..>3810
                     /locus_tag="PLJ02_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05382.1"
                     /translation="MKNLSWRVILLFLIAFSLVLSVVLIFYPEKTIEKQAIYLRPLDL
                     VAVYKNPNINQEHFWENLQLSAESVYAFDLGTATLLYEKNSRKRLYPAST"
BASE COUNT         1279 a          601 c          778 g         1152 t
ORIGIN      
        1 cgtcgccaaa attcttgagc agcagcacta tgtttaggat tggtggtgcg gataaaatga
       61 gtgttactca aatttaattc attttttaag ttttgaaact gttgagcaaa gtgatcgcag
      121 tagtcttgcg gatcaagacc tttagccttg gcttgatcag caattttttg cccgtgctca
      181 tcagtgccgg tattaaaaat tacttgttca tttaatatct ctcgatgata tctagcaatc
      241 acgtcagctt caacaatttc taaagcaaaa ccaatatgtg gtgatgagtt cacgtaaggc
      301 agagtggtgg tcacataaaa agttttactc atagaacctc tcattatagc agtcttttct
      361 cgatctggtt aacaataagc cagatgaatg gggggatggc taagattatt atccagtaat
      421 agttaaaatc gagattttgt aaatgaaaaa ccagcataat cagtaactca aaagctagac
      481 aaaaagctag acgcactttc tttaaagcga attttaaaaa aaagaaaaca gtgaaaaata
      541 ttaatataac aaagggcaag taagaattag cataaagcag attagccaca tcattgggtg
      601 acgtgtaaaa cattaagtat tgccaggcga ggaaacagac taaagccaag atgagatctg
      661 gcagcgaatt gctatttttg gcttttcgaa tttttcttct gtcaacagga attgcctgcg
      721 ttttcaggcg ggagtgtccc cgcagttttt tttgggtttg ggtagtttcc atagttttag
      781 tatacttaaa gaatatgaat cttgtcatta tcatttcttt ggccatctta attttattag
      841 tctcttttct tttatttttt ttactgaaaa aatcacaaaa gagtattgag gatgaaaaaa
      901 tcttggatga aaaattagag tcgacggtga ataaagtgtt tgggatgaca gctaacaaaa
      961 ttgcctatca aagcaaaaac attttacaga gtgagcagga tttattaaaa actgatttag
     1021 ccaacaaaca agcaactatc gaaaaattag ttaagcaact acaagaagat ttggaacaac
     1081 ggcaaaaaga aattagagaa attgagaaac aacagattga taaaattggt cgagtggcaa
     1141 cggcgattga agatcaccgg gttttaacca aggatctcaa agtttctaca gagaaattag
     1201 ctaagatatt agacaacaat caaacccgag gagcctgggg ggagcgtatt atcgaagatt
     1261 tattacaggc caatggctta caagaaggcg tgcattatgt acgacaaaca aaacaaagct
     1321 cttctaactt aataccagat atcaccttgt tgcttcccga caaacgtaat gtagcggtgg
     1381 atgttaaatt tccttatcag gaaattcaga aaatggccaa tactgatgat aaaactgaga
     1441 aaaaaaatca tttacaacaa tttgcaaggg accttagact aaaagtggaa aaggtcgctg
     1501 aatatatttc tcctgaaagc gacaccttgg attatgccat cctctttgtg cccaacgaaa
     1561 tgattttttc ttttattaat caaaagctac ccgatattat tgatgaagcg ctagctaaga
     1621 aagtgatttt gtgttcaccc tttagctttt tagtggtggc tagaactgtg atggaaagct
     1681 accggaactt tatggttggt aaaaaaattc aagaagtgat taaacaagtt gaagctttta
     1741 gtggtgaatg ggttaaattt gatgaagagt ttcaaaaatt tggcagaagc attgaaagtt
     1801 tacaaaaaaa ctttgataca ttaagtacca cccgcaaaaa acaaatggaa aaaagaattc
     1861 acaaaattga agaatatcga tcggggtctg gtcaactatt ggccgatggt cataaatcag
     1921 gtggtcctca ctaatcaggt tgttaacgtc tgcgccgttc ataaagaaac aagatcccag
     1981 tcaatgataa ttggtcagta atcgattttc ctcaagtggg ctaatttact attattgctg
     2041 attttattaa gcaaaaactg tcaattaggc tttataataa aacagtttat gtcaaaatta
     2101 aatttaagtc ggactaccgt catcgatagc aaaaacaaag ttagcgaaac tgtcaaatta
     2161 gctggttggg tggctaataa acgtgatcat ggaaaagtta cttttattga tttacgcgat
     2221 gaaactggtt taattcagtg tgttggcatt aatgataaat taaaggattt gaccattgaa
     2281 tcggtcgtag aagtgattgg tcaaattaat aagcgcccgg caaaactggt gaatcaagaa
     2341 atggccacag gtgagataga ggtgttcgtt gaagattacc aaattttaaa taaaagtatc
     2401 gaattaccga ttagtattaa tagttctggt ttagaaatta atgaagaact gcgtttacag
     2461 tatcgttatt tagacttaag acgtcagcgt ttacaaaaaa atttacgcct cagatcaaaa
     2521 ttaattaata gttttcgtca agaattaatt gatcaggatt ttgtggagat tgaaactcca
     2581 atgttaacca aatcaaccaa agaaggagct agagatttta ttgtcccctc ccgtttttat
     2641 cctggaaaat tttatgcttt gccccaaagt ccccaacagt acaagcaatt attaatggca
     2701 gctggttttg aaaagtattt ccaatttgcg cgttgtattc gtgacgagga tttgcgggct
     2761 gatcgaggtt ttgaatttac tcaactagat attgagctga gttttattga tcagccaaca
     2821 atcatgcaac ttttagaaaa tttagtgaaa aaagtttgcc agcagttaaa cgtgcaaatc
     2881 aaagatcaac agtttccagt agtgacttat caagaagcga tggagaaata tggcgctgat
     2941 cgttttgatt tgcgtacagc tgaagataaa gaacgaggag tgttagcttt tgcttgggta
     3001 gttaactttc cttttttcaa aaaagttaac aaagccgatc gagcagaagt tttggacagt
     3061 aaaagtaatt ggacttttac tcacaatccc ttttcaaatc caattcctga acatttggac
     3121 aatcatttgg caggcaagaa tgttgaacaa atcattgcta gtcaatatga cttagtttgc
     3181 aatggctatg aggtgggtgg aggtagttta cgtgctcatc ggcccgaagt attgagggca
     3241 acttttaaaa taatgggtta tagtgatgaa gaaattgaag agagtgttgg tcacatgtta
     3301 aaagctttta aactgggcac cccgccccat ggagggatag cctttggtat cgatcgtttg
     3361 ttaatgttga tcactggtga agataatttg cgagaaacga tggcttttcc tatgactgct
     3421 agcggccgca caagcgtcat ggatgctccc agttccgtta gtaaagaaca actggcggaa
     3481 ttaaaactaa gcgtgacagc tgataaaaaa tcgagttaga attaagcagt gaagaattta
     3541 tcttggcgag taatattgtt gtttttgatt gccttttcgt tagtgttgag tgtggtcctg
     3601 attttttatc ccgaaaaaac cattgagaaa caagccattt atttaagacc tttagattta
     3661 gtggctgttt acaaaaatcc taatattaat caagaacatt tttgggaaaa tttacaactt
     3721 agtgccgaaa gtgtttatgc ttttgattta gggactgcca ctttgcttta cgaaaaaaat
     3781 tcccgaaaaa gattatatcc tgcttctacc
//
LOCUS       DAOSEW010000066         3794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_22215_length_3794_cov_4.281359, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000066 DAOSEW010000000
VERSION     DAOSEW010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22215_length_3794_cov_4.281359"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /gene="rnc"
                     /locus_tag="PLJ02_02875"
     CDS             complement(<1..573)
                     /gene="rnc"
                     /locus_tag="PLJ02_02875"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOI05383.1"
                     /translation="MLPEFKNQDLLITALTHRSALNEKEQSGTTASESNERLEFLGDA
                     VLELASTIFLYHERPTDAEGMLTTYRSALVRTETLAELAKELGLDQRMYLSKGEEAGG
                     GRDNPGLLADLLEAVIGALYLDQGFRVVQDFLGQVLFPKFSEILASKAYRDQKSLLQE
                     VVQARSLPTPAYRVIKEEGPDHNKTFTVEVL"
     gene            complement(588..857)
                     /locus_tag="PLJ02_02880"
     CDS             complement(588..857)
                     /locus_tag="PLJ02_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05384.1"
                     /translation="MQLDPQFFSQVQDLLETVTVNSRQEIVPEASLEEDLGLNLEEDL
                     GRLVALINRHFEIELETETVMQELSEAGATVAELAKLIHDEYELG"
     gene            complement(847..1293)
                     /locus_tag="PLJ02_02885"
     CDS             complement(847..1293)
                     /locus_tag="PLJ02_02885"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOI05385.1"
                     /translation="MDKRHSRRVERMQILFARTFLKPMEPEMEPMILALPDRLWFAEL
                     VKELPDIDKQLAVYAPERPLTDINKVDLAILRLIVFESRTQDTPKKVLVDEAIELAKE
                     FGTDSSPKFVNGVLGKILFLKKATKKTPPKASKKTKKPAKKATNAT"
     gene            complement(1391..1549)
                     /locus_tag="PLJ02_02890"
     CDS             complement(1391..1549)
                     /locus_tag="PLJ02_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05386.1"
                     /translation="MGALPKNKITRVEQGKRRAGNKPSLKKDAKKASIPTHKQGLVAS
                     ILKKLDLN"
     gene            1696..2772
                     /locus_tag="PLJ02_02895"
     CDS             1696..2772
                     /locus_tag="PLJ02_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05387.1"
                     /translation="MSQIEHEQPTIANKRELPAFLKDLPYKVAQMKAKVYRALGLATV
                     AALMLTSCIPEGQVQETQPAVTQPIGEITHDTGVETELASATPEATATPENTPTTTAT
                     ATPKHTATPEFTATPKAMTQAEIRAEILAAGVNLDDLANSADEWTRSHMAIETIQNYI
                     DNLNFGRETEGSVTTVVIGLEAVENLAELDQALLTDAGWKLTTFAKLAYKDVNGDWQI
                     IKVPLNAYHAEDDLFWMKNVSTRMSPFFMDGKEIIQPDENGNLIIPLVNSWIAFSDLA
                     KYHSGTGSFIRLFTGWVEDPAYAGNDCILGDPPRYSQEQLIEFRKTGDPSVFGYQDSD
                     GYYIYWPLVTFNADLSALSAYNHD"
     gene            2819..3553
                     /locus_tag="PLJ02_02900"
     CDS             2819..3553
                     /locus_tag="PLJ02_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05388.1"
                     /translation="MNNSKLELVKNSWWKYLLLLLLLLVLVAVFYWRQTRFQQPISID
                     EPINNVETNNLSNFDFNTFPIEAGGELGIFYRLDCSYNELTIFRNYQQLPNLPGDWRC
                     LAEISCQYLNKNRELETIYLPLHIYQPSTQQLLFVGGSIKQVEETEVMMYVEKADQGF
                     YEAMMENALGKKPVKGDKIKIVANFPDEGFKNNETNGVAFDTLLEANPPYTQDDLIKL
                     YQTGDSQYLPQIDGKPYFWPVVGYSF"
     gene            3555..>3794
                     /locus_tag="PLJ02_02905"
     CDS             3555..>3794
                     /locus_tag="PLJ02_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05389.1"
                     /translation="MKKIIILIILGILSLISINQVVAIDYDQWCVPGVCWPQFGGSAV
                     FYDGCTEKWGYCAGTGEVAGMVCNCRQGQVGYAGKC"
BASE COUNT         1121 a          767 c          776 g         1130 t
ORIGIN      
        1 caagacttct acggtaaaag ttttgttgtg atctggacct tcttctttaa tgacgcgata
       61 agctggtgtc ggcagtgagc gagcttgaac tacttcttgt agtaaacttt tttgatcacg
      121 ataggctttg ctagctaaaa tttcactaaa tttgggaaac aacacttgac ctaaaaaatc
      181 ttgcaccacc ctgaagcctt gatctaaata caaagcgccg atcactgctt ccagtaagtc
      241 agcaagcaat cccggattgt ctcgaccccc accagcttct tcacctttac ttagatacat
      301 gcgttgatcc agacctagct ctttggccaa ttcagctaga gtttccgtac gcaccagggc
      361 actgcggtaa gtggttaaca tcccttccgc atcagtcggt cgttcatgat agagaaaaat
      421 ggtactggcc agctccaaca ccgcatcacc caaaaattct agccgttcat tggattcact
      481 agccgtcgtt ccagattgtt ctttttcatt aagcgcactg cggtgggtga gagcagttat
      541 tagtaaatct tgatttttaa actcaggtaa cataactcaa caccatttta acccaattcg
      601 tattcgtcgt gaatcagctt cgctaattcg gccacagtgg caccagcttc agatagctct
      661 tgcatcactg tttctgtttc gagctcaatt tcaaaatgac gattaatcaa agcaaccaaa
      721 cgccctaagt cttcttccag atttagccct aaatcttctt ctaaactcgc ctcgggcaca
      781 atttcttggc gactattaac cgtgactgtt tctagtaaat cttgaacttg ggaaaaaaat
      841 tgaggatcaa gttgcattgg tggctttctt tgctggtttc tttgtttttt tactggcttt
      901 tggaggagtc ttctttgtgg cttttttcaa aaacaaaatt ttgcccagca ctccattcac
      961 aaatttaggt gaactatcgg tgccaaactc cttagccaac tcaatcgctt catctaccag
     1021 cactttttta ggcgtgtctt gagtacgact ttcaaaaacg attaaacgta aaatggctaa
     1081 atcgactttg ttgatatcgg ttagtggtcg ttcaggggcg tagaccgcca gttgtttatc
     1141 aatatctggc agttctttga ctaattcagc aaaccacagc cgatctggca aagctaaaat
     1201 catgggttcc atttctggct ccattggctt taaaaaagta cgagcaaata agatttgcat
     1261 gcgctccacg cgccgactgt gacgtttatc catagcagta gattagggga aaaaagccaa
     1321 acttgcaata gagaaaatgc tgagctttga ctcgataatc aggctcgaac ctagtctaat
     1381 aaaatttatc ttaattaaga tcaagttttt ttaaaatact agccaccaaa ccctgtttgt
     1441 gggtaggaat agaggctttt ttagcatcct ttttgagact gggtttattg cccgctcgac
     1501 gcttaccttg ttcaactcta gtaattttat tctttggtaa tgctcccatg agatcctttt
     1561 tgttgcaaaa cttttgtcaa taagggcttt attttaaggg taatgggaga aaaaggcaag
     1621 gggagaggag ataattgtga tataattagt ctttgttaga tcctgaataa ttaactaaat
     1681 aaagggataa ataccatgag tcaaattgaa cacgaacaac caacaattgc taataaacga
     1741 gaactaccag cttttttaaa agaccttccc tataaagtcg cgcaaatgaa agcaaaagtt
     1801 tatagagctt tgggcttggc tactgtagct gcgcttatgc tgacttcctg catcccagag
     1861 ggacaagttc aagaaactca gccagcagtc actcaaccca ttggagaaat tacccatgat
     1921 acaggagtgg aaactgagct ggctagtgcc actccagaag cgactgccac cccagaaaac
     1981 accccaacca ctacagccac agctacgcca aagcatactg ccactcctga gttcactgca
     2041 actccaaagg cgatgactca agcagaaatt cgggcagaaa ttttagcggc cggggttaat
     2101 ttggatgatt tggccaattc agcagacgag tggaccagga gtcacatggc tattgaaacc
     2161 atccaaaatt acattgataa cctcaatttt ggcagagaaa ctgaaggttc tgtaaccacg
     2221 gtggtaattg gtctagaagc agtagaaaat ctagcagaac tggatcaagc actactcact
     2281 gatgctggct ggaaattaac tacctttgcc aaactggcct ataaagatgt taatggtgat
     2341 tggcaaatca taaaggttcc actgaatgcc tatcatgctg aagacgactt gttttggatg
     2401 aaaaatgttt ctactcgaat gagtcctttt tttatggatg gaaaagagat catacaacca
     2461 gatgaaaacg gaaatcttat tatcccgctt gttaatagtt ggattgcctt cagtgatctt
     2521 gccaaatatc attcaggtac tggtagcttt atcagattgt ttactggttg ggtagaagat
     2581 cctgcatacg ctggcaacga ttgtatcttg ggtgatccac ctcgctacag ccaagaacaa
     2641 ctgattgaat ttagaaaaac tggtgatccg agtgtttttg gctatcaaga tagtgatggt
     2701 tactacattt attggccact cgttactttt aatgcggatt tatctgcact cagtgcttat
     2761 aatcatgact agtttggcaa atcaataaat tgatcaattt ttattattga ctactagagt
     2821 gaataactct aaactagagt tagtgaaaaa ttcttggtgg aaatatttgc tgttgttact
     2881 gttgctactg gtgctagtgg ctgtatttta ttggcgccaa acgagatttc aacaaccaat
     2941 cagtattgat gaaccaataa ataatgttga aacaaataat ttgagcaatt ttgacttcaa
     3001 cacttttccc atagaggcag gaggcgagct aggaatattt tatcgtcttg attgcagtta
     3061 caatgagttg actattttcc gcaactacca gcaattacct aatttgcctg gtgattggcg
     3121 ctgtctggct gaaattagtt gccaatattt aaataaaaac cgagaattgg aaactatcta
     3181 cttgccccta catatttatc agccaagcac ccaacagcta ctttttgtcg gaggcagtat
     3241 caaacaggta gaagaaactg aagttatgat gtatgtggaa aaggctgacc agggtttcta
     3301 tgaagcgatg atggaaaacg ctttaggaaa aaaacctgtc aagggagata aaattaaaat
     3361 tgtagccaat tttccagatg aagggtttaa aaataatgaa actaacggag tcgcctttga
     3421 cactctacta gaagctaacc ccccatatac tcaagatgat ttaatcaaac tttatcaaac
     3481 tggcgacagc caatacctac ctcaaattga tggcaaacct tatttttggc cagtcgttgg
     3541 ctatagtttt taaaatgaaa aaaataatta tcctaattat ccttggcatt ttgagtttga
     3601 tctcaattaa tcaagttgtt gccattgatt atgatcaatg gtgcgttcct ggtgtttgtt
     3661 ggccacaatt tggcggtagc gctgtatttt atgatggatg tactgaaaag tggggttatt
     3721 gcgctggtac aggcgaggta gcaggcatgg tttgcaattg caggcagggg caagttggct
     3781 acgctggtaa gtgt
//
LOCUS       DAOSEW010000067         3519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_24735_length_3519_cov_3.615762, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000067 DAOSEW010000000
VERSION     DAOSEW010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24735_length_3519_cov_3.615762"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /gene="mltG"
                     /locus_tag="PLJ02_02910"
     CDS             <1..586
                     /gene="mltG"
                     /locus_tag="PLJ02_02910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOI05390.1"
                     /translation="SEFDEQEFLVLTDGLEGQLFPDTYLVPKASTTASIVNLLVNTFE
                     SKVSTALAEDLASSQLDLNQILTLASLVQRESANEQDMPLIAGILFNRLEINMPLQVD
                     ATLQYVKGYSVAEQTWWSNPVAADKNLASAFNTYQNPGLPPQPICNPGLAAIKAVLHP
                     QSTTALYYIHDLADQIHTADTIEGHNANVNQYLR"
     gene            859..>3519
                     /gene="rpoB"
                     /locus_tag="PLJ02_02915"
     CDS             859..>3519
                     /gene="rpoB"
                     /locus_tag="PLJ02_02915"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOI05391.1"
                     /translation="MPQTSNRQNWGQEHSILPELDLVALQINSYQDFLNNGIRESLKE
                     INGERGIEDFTGKNWSLKFGDYRFGPAKYTPTEAKTKNVSYDQPLYVEATLLNKKTGE
                     ERTQEVFLGDIPAMTNTGTFIINGIERGIVTQLVRSPGVFFSGEEDQNTKELLYKAEL
                     RPMRGSWLEINVSRRHVLTIKIDRRRKMPATVLLRALGWETNDDIAEALAEVLKNDEL
                     DLINNTLRKDSATSQAEALIEIYEKMRPGEPAVLETAREFLKNMFFDPRRYDLGEVGR
                     YKMNRRLGIKLDEKLTVLAKEDVLASIKYLIKLQNGEGKVDDIDHLSNRRVRQVGELV
                     SISAFRIGLIRLERSIREKMSLTKTDEVLTPSALVNARPLIASIAEFFRRNRLSTILD
                     QTNPLSEIDNLRRLSVMGTGGVTRDRASFSMRDINASQYSRICPIRSPEGPNIGLVTY
                     LALYTRVNKYGFLEAPYAKVINYRGKMKVTDEIVYLSADEEEDYYITHAGVDIDDKGY
                     ITQDWVPMRYNNQFIEGSVQKVQYIDVVPRQVVGCSASLIPFVAHDEANRALMGSHMQ
                     CQAVPLVKTQAPIVGTGMEKEIATAMGWTVLAEFPGEVLYVDASEVVLKVDAKYQEKA
                     KAWVETANNERVSYQQGNLTYKLNKFARTSQSTCYNQTPEVQVGDKIKKGDVLIDGPA
                     TDHGELALGTNLLIAYTCFKGLGYEDAIVISDRLVQQDTLSSIQISEHKAKVMDTRLG
                     PEELTNDIPNVSETFLANLNTDGIIQIGSHVKSGDILVGKVAPKGETELSPEERLLRA
                     IFGEKSKEIRDTSLRMPHGGEGTVIDVQILDRDLGDELDPGTIKEVIVKIAQLRKIVV
                     GDKLAGRHGNKGVISKIVPQADMPHLEDGTP"
BASE COUNT         1113 a          587 c          740 g         1079 t
ORIGIN      
        1 tagtgaattt gatgaacaag aatttttggt gttgaccgat ggcttggaag gacaattgtt
       61 ccccgacact tatctagtgc caaaagcgag tactactgcc agcattgtga atctattagt
      121 taataccttt gaaagcaagg tatcaactgc tctagctgaa gatttagcta gcagtcaatt
      181 agacttgaat caaattttga ctcttgcctc tctagttcaa agagagagtg ctaatgagca
      241 agatatgcca ctaatagctg gtattttatt caatcgtttg gaaattaata tgccactgca
      301 agttgatgcg accttgcaat atgttaaggg ttatagtgtt gctgagcaaa cgtggtggtc
      361 aaatccagta gctgcagaca aaaatttagc ttcagcattt aatacttacc aaaatccagg
      421 attgccacct cagccaatat gtaatcctgg cttagcagcg attaaggcag ttcttcatcc
      481 tcagtcaaca actgccttat actatatcca cgatcttgcc gaccaaattc acacggcaga
      541 cacgattgaa ggtcacaacg ctaatgtaaa tcaatacttg cgctaattct tgcttcctgt
      601 tacaatgcac aattgtctaa aaagagattt tagcctaaag caataacaag tagcagaact
      661 aatttgtgac ttttgttcta aaaaacaata aaacaactaa ttaattttcg cacagcaagt
      721 aattgataat cttggctgtg tttttttgct atggttaagc tgtttttagg acgagcttaa
      781 aatactttaa ttttaaattt aaaacacagt ttaaattatt aaaaatcaac aataaagaaa
      841 atttagcaag ggattttcat gccacaaacc agcaatcgcc aaaattgggg acaagaacat
      901 tcaatcctac cagaattgga tctagttgcc ctgcaaatta atagttatca agatttttta
      961 aacaatggga tcagagaatc tttaaaggag attaatggag aacgaggtat tgaagatttt
     1021 actggaaaaa actggtcact gaaatttggt gactatcgtt ttggtccggc aaaatataca
     1081 cctacagaag caaagaccaa aaatgtttct tacgatcaac ccctatatgt tgaggcgact
     1141 ttattaaaca aaaaaactgg tgaggaaaga actcaagaag tctttttggg tgatattccg
     1201 gccatgacca ataccggtac ctttattatc aatggtatcg aaagaggcat tgttactcaa
     1261 ttggttagat caccaggcgt tttcttttct ggtgaggaag atcaaaacac caaagaattg
     1321 ctttataaag ctgagctgag gccaatgcgt ggttcttggt tagagattaa tgttagtcgt
     1381 cgtcatgttt taaccattaa aattgatcgt cgacgcaaga tgccggctac cgtgctgctt
     1441 cgcgctttgg gttgggaaac taatgatgat atcgcagaag ctttagctga agttttaaaa
     1501 aatgacgaac tagatttaat taacaacact ttacgtaaag attcagctac tagtcaagct
     1561 gaggctttaa tcgaaatcta cgaaaaaatg cgtccaggtg aaccagcggt tttagaaaca
     1621 gctcgtgagt ttttgaaaaa tatgtttttt gatcctcgtc gctatgattt gggtgaagta
     1681 ggtcgttata aaatgaatcg tcgcttaggg atcaaacttg atgaaaaatt aactgttttg
     1741 gctaaagaag atgttttggc tagtatcaaa tatttaatta aattacaaaa cggcgagggt
     1801 aaagttgatg atattgatca tctttctaat cgtcgcgtac gccaagttgg agaattagtt
     1861 tcaatcagtg cttttcgtat tggtttaatt cgtttagaaa gatctattcg tgagaaaatg
     1921 tcattgacca aaactgatga agtattaact ccctcagctc tggttaatgc tcgaccactg
     1981 attgcctcta ttgctgaatt tttccgtcgt aatcgtttat ctaccattct tgatcaaact
     2041 aatcccttat cagaaattga taatctgcgc cgtttgtcag tgatgggtac tggtggtgtg
     2101 accagagatc gtgcttcttt ttcaatgcgt gatattaatg cgtcacaata ttcaagaatt
     2161 tgtccaattc gttctccaga gggtccaaac atcggtttgg tgacttactt agctctttat
     2221 accagagtta ataaatatgg atttttagaa gcaccttacg ctaaggtaat taattatcga
     2281 ggtaagatga aagtgactga tgaaatagtt tacttgagtg ctgatgaaga ggaagattat
     2341 tacattactc acgctggagt tgacattgat gataagggtt atattactca ggattgggtg
     2401 ccaatgcgct ataacaatca atttattgaa ggttcggtac aaaaggttca atacattgat
     2461 gtggtgccac gtcaagtggt tggttgtagt gcctcgctga ttccttttgt tgctcatgat
     2521 gaagctaacc gtgctttgat gggttcacac atgcaatgtc aagcagtgcc tcttgttaaa
     2581 actcaagcac caattgtggg cactggtatg gaaaaagaga tagcgacggc gatgggttgg
     2641 acagttttag cagaatttcc tggtgaagtc ctttatgttg atgctagcga agtggtatta
     2701 aaagttgatg ccaagtatca agaaaaagcc aaagcttggg tggagactgc taacaatgag
     2761 cgagtgagtt atcagcaagg taatttaact tacaagttaa ataaatttgc tcgtacctct
     2821 caaagtactt gttataatca aactccagaa gtgcaagtgg gagataagat taaaaaaggt
     2881 gatgttttaa ttgatggtcc agctactgat catggagaat tagctttagg taccaatctg
     2941 ttaattgcct atacttgttt taaaggtttg ggttatgaag acgctattgt gatttctgat
     3001 cgtttagtgc aacaagatac tttaagttct attcaaatca gtgaacacaa agccaaagtg
     3061 atggacactc gcttaggtcc tgaagaatta actaatgaca ttcccaatgt cagtgaaact
     3121 tttttggcta acttaaatac tgatggtatt atccaaatag gtagccatgt taaatctggt
     3181 gacattttag taggaaaagt agctcctaaa ggtgaaactg aactttcacc agaagaacgt
     3241 ttattaaggg ctatttttgg tgagaaatca aaagaaatcc gagatacttc attgcgtatg
     3301 cctcacggag gagaagggac agtgattgat gtccaaattt tagatcgtga tttgggtgat
     3361 gaattggatc cgggaacaat taaggaagtg atagttaaaa ttgctcaatt aagaaaaatt
     3421 gtggttggcg ataaattagc cggccgtcac ggtaataagg gagtgatctc caaaattgtt
     3481 cctcaagcag atatgcctca tttagaggac ggtacgcca
//
LOCUS       DAOSEW010000068         3320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_26880_length_3320_cov_3.378254, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000068 DAOSEW010000000
VERSION     DAOSEW010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26880_length_3320_cov_3.378254"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PLJ02_02920"
     CDS             complement(<1..378)
                     /locus_tag="PLJ02_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOI05392.1"
                     /translation="MTDIQKLPASQLQPNPFQPREKVKKEDLMDLIESIRIHGILEPL
                     VVAETPAGYQIIAGERRWRAAKVLGIEEVPVYVKKTSPQGMLEMAIVENVQRTDLNPI
                     ERAQSFARLNREFGLSINELAKRI"
     gene            complement(489..749)
                     /locus_tag="PLJ02_02925"
     CDS             complement(489..749)
                     /locus_tag="PLJ02_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008202895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOI05393.1"
                     /translation="MAHVKGSGSVTQHSQGARHGRRFGLKKFGGENIQVGQIILRQKG
                     AKYKAGAGTKLGKDYTIFAMVDGVVDFTKHFGKTVVKVIAKS"
     gene            complement(762..1943)
                     /locus_tag="PLJ02_02930"
     CDS             complement(762..1943)
                     /locus_tag="PLJ02_02930"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05394.1"
                     /translation="MSKFLFLFGNTPQLSLLELNTVYPDLTVRHFAGGELAGRLALVE
                     LDKQEFEQANFMQRLAGVVKIFEVEKILNFSISLGELKNEIVASLLAKSQQPHFSIYQ
                     KGKGQKAINHAQIKDLLKDRGYRSRYYKGSLDGSAFSLHEEASQEIFLYHSEEGIFLS
                     HLIAVQNIDDWTKRDRAKPYFNRKKGMLPPKVARMLVNIAGSTWLRENEQRTLTEAKL
                     FDPFCGTGTVLIEGLAVGCQVYGADLDEQAVWGTRENLQWFCDEYQVKSQDFAKQIFL
                     ADVGQLDLEKLGGQKVDLLVTEPFLGRQTPREEELANIFKGLEKLYLGAFNNFSNILN
                     PGAKIAIIFPKVVAEHQVFSLEHLIDKLSAKGYNLLASPLLYARSGARVQRELYFFNF
                     K"
     gene            complement(1949..2260)
                     /gene="rplU"
                     /locus_tag="PLJ02_02935"
     CDS             complement(1949..2260)
                     /gene="rplU"
                     /locus_tag="PLJ02_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006691324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOI05395.1"
                     /translation="MKYAIIQLQGKQFQVSEGDEIIVDRLDQAEGDQFTVNDVLLVVD
                     GDKRKIGQPIVKGAEVKCELISNDKGKKIRVAKYKAKSRYRKVQGHRQLQSVVKILQI
                     N"
     gene            complement(2291..>3320)
                     /locus_tag="PLJ02_02940"
     CDS             complement(2291..>3320)
                     /locus_tag="PLJ02_02940"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOI05396.1"
                     /translation="NIQLLAYLLGFLLFFSFSKIAVAYWWRFSPYLYWALNVVLLALL
                     IWGRATRGIVSWIELPLGLRFQPSQLAVPLTALYLSSRFKNQEQTAQVQILSWWQIIE
                     MLAIIFLPAVLINLQPDFGTAIVYLLALLVFLFFNRLGWQKLVVFLSVGVLGAVVVWF
                     FVLADYQKSRLTSFLVFNSPERSLSSDFRQESSAAYNARQAVIAVGAGQLWGRGLGQG
                     TQSHLKFLPERQTDFIFASFVEEWGFLGSLLILGLYFALLTFLLYLVYVEENRGKKLY
                     MLSILMMLITQIFINIGMNLALLPITGITLPFLSYGGSSILALFYCLALVQSMLLESD
                     KPPSSIFQ"
BASE COUNT         1082 a          749 c          543 g          946 t
ORIGIN      
        1 aattcttttg gctaactcat taatggataa accaaattct cggttaagcc gtgcaaaaga
       61 ttgtgcccgt tcaatcgggt tgaggtcggt acgttgcaca ttttcaacaa tagccatttc
      121 caacatcccc tgaggactgg tttttttcac gtaaactggc acctcttcaa tacccagtac
      181 tttggctgcc cgccaccgtc tttctcccgc aataatttga tagcccgctg gagtttcagc
      241 gaccactagc ggttctaaaa taccgtgaat tctgatggat tctatcaaat ccattaagtc
      301 ttcttttttg actttttcac gaggctgaaa aggattgggt tgtaactggc tggctggaag
      361 tttttgaata tctgtcatac tctatgtata acataatata tcaattttgc ctagagtttt
      421 tttcacaatt ttcacaagca atctttgctc accagcaaac aagtcaagtt agagaaaaaa
      481 cagcgagttt agcttttagc gatcactttg accacagttt tgccaaagtg tttagtaaaa
      541 tcgactacac catcaaccat agcaaaaatt gtgtagtctt tacctagttt ggttcctgcg
      601 ccagctttat atttagcacc tttttgacgc agaataattt gaccaacttg gatattttcc
      661 ccaccaaatt tctttaaacc aaaacgacga ccgtgtcttg caccctgcga atgttgtgta
      721 acgctacctg aacctttaac gtgtgccata tttcctttgt attacttaaa attaaaaaaa
      781 taaagttctc gctgcactct ggctccacta cgagcataga gtagaggcga agctaaaaga
      841 ttgtagcctt ttgcgcttaa tttgtcaatt aaatgctcaa gagaaaaaac ttgatgctca
      901 gccacaacct tgggaaaaat aattgcaatt ttggctccag gattaagaat gttactgaaa
      961 ttattaaatg cacctaaata taatttttct aaacccttaa aaatattagc taattcctct
     1021 tcccttgggg tctgtcgacc caaaaaaggc tcagtgacta ataaatcaac tttctgacca
     1081 cctaattttt ctaaatccaa ttgtccgaca tcggccaaaa aaatctgctt ggcaaaatct
     1141 tgacttttca cctgatattc atcacaaaac cactgtaaat tttctctagt accccaaacc
     1201 gcctgctcat ctaaatcggc gccatagacc tgacaaccca cggctaatcc ttcaatcagc
     1261 accgtccccg ttccacaaaa aggatcaaat agtttagctt ctgtcagggt gcgttgttca
     1321 ttttccctca accaagtact gccagcaata ttgaccaaca tccgtgccac ttttggtggc
     1381 aacatccctt ttttccgatt aaagtagggt ttagcacgat cacgcttcgt ccagtcatca
     1441 atgttttgca ccgcaattaa atgacttaaa aaaatgcctt cttcactgtg ataaagaaaa
     1501 atttcctgac ttgcttcttc gtgtaaagaa aaggcactcc catctagtga acctttataa
     1561 tagcgggagc gataacctct gtctttgagt aaatccttga tttgagcgtg attgatggct
     1621 ttttgacctt tacctttttg gtaaatacta aaatgaggtt gctgactttt ggctagtaaa
     1681 ctagctacta tttcattttt taattcacct aaactaatgg agaaattgag aattttttcc
     1741 acctcaaaaa ttttaactac acctgctaaa cgctgcataa aatttgcttg ttcaaactct
     1801 tgtttatcca gctcaaccaa ggctaaacgt cctgccaact caccaccagc aaaatggcgc
     1861 accgttaaat ctggataaac agtattgagc tccaataacg acaactgggg ggtattacca
     1921 aacaaaaata aaaacttgga cataggtcct aattgatttg caaaatttta accacgcttt
     1981 gtaattgacg atggccttga actttgcgat aacgagattt agccttatat ttagctactc
     2041 ggattttttt acccttatca ttgctgataa gctcacattt aacctcagct cctttaacaa
     2101 taggctgacc gatttttctc ttgtccccgt caaccaccaa caaaacatca ttaaccgtaa
     2161 attgatcacc ttctgcttga tctaagcgat cgacaataat ttcgtcacct tcactcacct
     2221 gaaattgctt accttgtaat tgaataattg cgtatttcat aacgatctgt gattataaca
     2281 tatcttaatc ttattgaaag attgagctgg ggggtttatc actttcaagc agcatacttt
     2341 ggactagggc caaacaataa aataaagcca agatactgct accaccataa gataaaaagg
     2401 gcagagtaat gccggtgatc ggtaataagg ccagattcat cccaatattg ataaaaattt
     2461 gagttatcaa catcattaaa atacttaaca tatacagctt tttgccacga ttttcctcaa
     2521 cgtacaccaa atataaaaga aaagttaata aagcaaaata taagccaaga attaataaag
     2581 aacccaaaaa gccccattct tcaacaaaag aagcaaaaat aaaatcagtt tgtctttctg
     2641 gtaagaattt taagtgagat tgagtgccct ggcccaagcc tcgaccccaa agttgaccag
     2701 cgccaacagc aatgaccgcc tgccgagcat tataagcagc actgctttcc tgacgaaaat
     2761 cactactaag gctcctttct gggctattaa aaactaagaa actggtcaag cgtgattttt
     2821 ggtaatctgc taaaacaaaa aaccaaacca ccaccgcacc caacacaccc accgatagaa
     2881 aaacaacgag cttttgccaa cccaagcgat taaaaaatag aaaaaccagc agagctagca
     2941 aatagacaat ggcggtgcca aaatccggtt gtaaattaat caagacagcg ggtaagaaaa
     3001 taattgccaa catctctata atttgccacc aagataaaat ttgcacttga gcggtttgct
     3061 cttgattctt aaagcgacta gacaaatata aggcggtcaa aggaacggct aattgagatg
     3121 gttggaaacg cagacccaaa ggcagttcaa tccaagaaac aattcctcga gtagcccgac
     3181 cccaaattaa caatgccaac aatacaacat ttaaagccca gtaaaggtac ggactaaacc
     3241 gccaccaata agccaccgca attttggaaa aactaaaaaa caataaaaac cctagtaagt
     3301 aagcaagcag ctgaatgttt
//
LOCUS       DAOSEW010000069         3307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_27023_length_3307_cov_2.964022, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000069 DAOSEW010000000
VERSION     DAOSEW010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27023_length_3307_cov_2.964022"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="PLJ02_02945"
     CDS             complement(<1..752)
                     /locus_tag="PLJ02_02945"
                     /inference="COORDINATES: protein motif:HMM:NF016104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HOI05397.1"
                     /translation="MQAAWKRILLIVLGWRLLLFLIGAVAPQFFAYQGSFSTPSVLQN
                     YQLPAWVNRWASFDGVHYLSIIEQGYKKIGLIQAFFPIYPLLAKWLGLLINNNLLSLL
                     IINNLATLLALYLLYVLNRQYFKRSQQFLPIILFLIFPSSFFLGAAYSEGLFLLWIFA
                     AFYFFGQKKFWPTALFIALASATRIVSIILLPAMLIDLFYQNKKKIDYSASFILCVGA
                     LGLVSYMGYLWVNFNDPLYFLTVQSQFGAGRQ"
     gene            complement(755..1810)
                     /locus_tag="PLJ02_02950"
     CDS             complement(755..1810)
                     /locus_tag="PLJ02_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOI05398.1"
                     /translation="MKVLLDTSPLSDANATRGIGTYTRFLLDRLLNNHSLEVYQSGAM
                     SEDELKKFKADVVHYPYFDFFFPTLPLKLITKTVVTIHDVIPLVFPQHYRPGKIGKVN
                     FLRQKLALKAVNRVITDSQSSKQDIIKHLGVPEDKITVIYLAANPKLQPLTQEVWQKY
                     QRQLRLPKKYILYVGDINYNKNLPQLIKSLKFLPNDIKLVCVGNNFHPQEIPEWLAIE
                     KQIALSDVAKRIKFIEDIVSDDPEELAAIYQGALAYLQPSIYEGFGLPILEAMQCRTP
                     VICGKNSSLPEIAGQRALFVEAEEAENFAAAVKQVLAWQKKERQAFVDQAAQWAKKFS
                     WEKTAKQTIKVYQELVQ"
     gene            complement(1811..2599)
                     /locus_tag="PLJ02_02955"
     CDS             complement(1811..2599)
                     /locus_tag="PLJ02_02955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOI05399.1"
                     /translation="MISVVLATHNEAHNIDKCLNSVKNFADEIIVVDGNSTDETRSLA
                     KKFGARVIKTTNKKNFHINKQLAMDKAKGQLVLQIDADEAVDEELADFIKKTDQRVSK
                     QKKFGHNDTVAWWIKRRNLFMGRWLKKGGQYPDPVIRLYVNGKAGLPQKDIHEQMWVD
                     GHIGMADGHLWHYSNPTFSVYLHKFNTYTSFKAQQLLESGLKINPITFINYLLIKPLV
                     TFLKLYLRHKGIVDGWPGFVFALFSGLHHPIAYLKCWELTLGEN"
     gene            complement(2620..>3307)
                     /locus_tag="PLJ02_02960"
     CDS             complement(2620..>3307)
                     /locus_tag="PLJ02_02960"
                     /inference="COORDINATES: protein motif:HMM:NF017555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HOI05400.1"
                     /translation="LGWYQPGERLHLKHGLYRFSDGKMSTRKGQVIWLNEVLDKAQAK
                     AEELCGDRETAKVLSIAALKWNDLKRNSQLAVTFNWDEVLNLQGNSGPYLLYAYARAK
                     SILAKAKEQGIDVSNRDVSNKWDSQALLAANLTMEPLEKAIIQTLTNFAKVVQQSQRQ
                     LNPALLCQYLYNLASQFNSFYNKYQILQDSSGEEAILRRDFRLRLVLQVSLVLKTGLN
                     ILGIETLEHL"
BASE COUNT          985 a          684 c          647 g          991 t
ORIGIN      
        1 ctttgacggc ctgctccaaa ttgactttgc acagtcaaaa aatataaagg atcattaaag
       61 ttgacccata aataacccat gtaactgact aaacccaacg cgcccacaca taaaataaaa
      121 ctagctgaat aatcaatctt ttttttgttt tgataaaata aatcaatcag catcgctggt
      181 aataaaataa tgctgacgat tctagtcgca cttgctaagg caatgaacaa ggcagtcggc
      241 caaaactttt tttgaccaaa gaaataaaaa gcggcaaaaa tccatagtaa aaataaacct
      301 tcgctatatg ctgcgcccag aaaaaaactg ctggggaaaa ttaaaaacaa aatgatgggt
      361 aaaaattgtt gtgagcgctt gaagtattgc cgattcaaca catataacag gtacaaagct
      421 aagagcgttg ccaaattatt aatgatcaat aaacttaata aattattatt gatcaacaag
      481 cctaaccatt tagctaatag cggataaatg gggaaaaaag cttgaattaa acctattttt
      541 ttataacctt gctcaataat gctgaggtaa tgcacaccat caaaactcgc ccagcggttg
      601 acccaagctg gtagctggta attttgcaaa acactaggag tagaaaaact gccttgataa
      661 gcaaaaaact gcggagctac agcaccgatt aaaaacaaca ataaacgcca gcccagcacg
      721 attaataaaa ttcttttcca agctgcttgc atgtttattg aacaagttct tgataaactt
      781 taatagtttg cttagcggtt ttttcccaag aaaacttttt ggcccactgc gcagcttggt
      841 caacaaaggc ttgccgttcc tttttctgcc aagctaacac ttgtttgact gctgcagcaa
      901 aattctctgc ttcttctgct tccacaaaca gcgctcgctg accagcaatt tctggtagtg
      961 atgagttttt accacaaatg actggagttc ggcactgcat cgcttccaaa attggtaaac
     1021 caaatccttc atagattgat ggttgaagat aagccaaggc gccttgataa atagcggcta
     1081 attcttctgg atcgtcgctg acaatgtcct caatgaattt aatgcgtttg gccacatcac
     1141 tcagggcaat ttgtttctca atggccagcc attccggaat ttcttgagga tgaaaattgt
     1201 tgccaacgca aactagtttg atgtcgtttg gtaaaaattt taggcttttg attaattgcg
     1261 gtagattttt gttgtaattg atatcaccca cataaagtat gtattttttg ggcaaacgca
     1321 gttgacgctg atatttttgc caaacttctt gggttaaagg ctggagtttg ggattagctg
     1381 ctagataaat aacggtgatt ttgtcttcgg gaacgcccaa gtgtttaata atgtcttgtt
     1441 tggacgactg actatcggta atgacgcggt tgaccgcttt cagggccagt ttttggcgca
     1501 aaaagttgac tttgccaatc ttgcctggtc gataatgttg cggaaagact aaaggaatta
     1561 cgtcgtgaat ggtgactacc gttttggtga ttaatttgag tggtaaagtg ggaaagaaaa
     1621 aatcaaaata cggatagtgc accacgtccg ctttgaattt ttttaattca tcttcgctca
     1681 ttgctcccga ttggtacact tccagactgt gattattgag caagcgatca agcaaaaacc
     1741 tggtataggt tccaattccc cgagtggcat tggcgtcact gagcggacta gtatcaagta
     1801 aaactttcat ttaattctct cccagtgtca attcccagca tttcaaataa gcgatcggat
     1861 gatgtaaacc actaaatagg gcaaaaacaa agcctggcca accatccacg atccccttat
     1921 ggcgcaaata aagttttaag aaagtcacta gcggtttgat caacagataa ttaataaagg
     1981 tgatcggatt aattttgagt cctgactcta gtaattgttg ggctttaaaa gaagtatagg
     2041 tattaaattt gtgcaaatag acggaaaaag tcggattgct ataatgccac aagtgaccat
     2101 cggccatgcc aatgtgacca tcaacccaca tttgttcgtg aatatctttt tgcggtagac
     2161 cagccttacc gttaacatac agccgaatga cgggatcggg atattgacca ccttttttta
     2221 gccagcgacc catgaataaa ttacgccgtt taatccacca cgctacggtg tcgttgtggc
     2281 caaacttttt ttgtttactt actcgctgat cggttttttt aataaagtcg gccagttctt
     2341 catccacggc ttcatcagca tcgatttgca acaccaattg ccctttggct ttatccatgg
     2401 ccaactgttt attaatgtga aagttttttt tgttggtggt tttgattacc cgcgccccaa
     2461 attttttagc taagctgcga gtctcgtcag ttgaattacc atccaccaca attatttcat
     2521 cagcaaaatt ttttactgaa tttaaacact tgtcaatatt gtgggcttcg ttgtgggtgg
     2581 ctaaaacgac tgaaatcata gtatttctat tataacaaat tacaaatgct ctaaagtctc
     2641 tatccccaag atatttaagc cagttttgag gactaaagat acttgaagca ccaatctcag
     2701 ccggaaatca cgccttagaa tcgcttcttc tccagaggaa tcttgcaaaa tttggtattt
     2761 attgtaaaaa ctgttaaatt gactagctaa attatataaa tattgacaaa gtagcgcggg
     2821 atttaattgg cgctgacttt gctggacgac tttggcaaaa ttagttaaag tttggataat
     2881 cgctttttca agcggctcca tagttaaatt agcagcaagc agtgcttggc tgtcccattt
     2941 gttgctgaca tctctattgc tgacgtcaat cccttgctct ttcgctttag cgagaatact
     3001 tttggcccgc gcgtaggcat agagcaagta gggaccagaa ttgccttgca aatttaacac
     3061 ctcatcccaa ttgaaggtca ccgctagttg gctgttgcgt ttgagatcat tccattttag
     3121 ggcagcaata ctcaacactt tggctgtctc ccgatcacca cacagctctt ctgctttggc
     3181 ttgagcttta tctaacacct cattgagcca aatcacttga cctttacggg tcgacatttt
     3241 gccatcgctg aaacggtata aaccatgttt aagatggaga cgctcgcccg gttggtacca
     3301 gcccagc
//
LOCUS       DAOSEW010000070         3286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_27284_length_3286_cov_3.774373, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000070 DAOSEW010000000
VERSION     DAOSEW010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27284_length_3286_cov_3.774373"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PLJ02_02965"
     CDS             complement(<1..488)
                     /locus_tag="PLJ02_02965"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOI05401.1"
                     /translation="MKKHYYLENFLYFVGRSIARLYTKIDLKKQIKSKKQFPLGAKIF
                     AVNHPSTLDPLYIMSSIKEPVHALLTEDVFKIFGLNKLIAWAGHVAVGRNSGLIALNQ
                     AITLLTKNRSILIFPEGMVSKNSQQVEKLKTGAVRMALATGAPIIPIGICVDPQKIKK
                     FT"
     gene            complement(563..1696)
                     /locus_tag="PLJ02_02970"
     CDS             complement(563..1696)
                     /locus_tag="PLJ02_02970"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOI05402.1"
                     /translation="MFKKVRQTLTKKLRKFTKKQKTVLYVLLCLLILIISISATVAYQ
                     HFQQQKQTQDEILIEAQATHPLNSDTSQIEENDRKELNVLLIGYGGAGHQGGMLADLI
                     QIAHFDFEKNTLAFISIPRDLELSLGNGRYQKINNVFSSQMTGSDPIVNAGQVTKNLL
                     SSITNLNIKYFIAVDFVGFQRLIGQELGSIEVEVAEDFKDQWYPIEGKQLDPCGYSNE
                     EIAQLTATLSGFELEKQFTCRYETIYFPAGLQKMEGAEALKYVRSRHSSSDFDRSRRQ
                     VEVLTGIRKKLFELEALSHIPDFFKKVNQHVTTDLNLEILEYLAPLLLNANDFTIKNI
                     NLSTENVLNSSSSSNGFVLIPKAGPNNWTQLQNFIQDSLNSDN"
     gene            1734..2450
                     /locus_tag="PLJ02_02975"
     CDS             1734..2450
                     /locus_tag="PLJ02_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05403.1"
                     /translation="MSNQSFFDLKKNNFPYDQSRFPGQASDERILYVTREHPLFLFLR
                     LAFVLLVGLSIILAGSILNRTLNAVMTNQLLSELILVILVIFFIFMVIGVWWVFTLWK
                     KSVAIVTNKRLIKFIYTTPFNRYNMSLPLEMIVDTSFHNRGFLSSYLKLGSITARSSA
                     SSSGVATDDPARVNKKYFYIENIQYAEDLQQYLNKLINTLHKEPKELANFRPFLPNLK
                     GEAREDFLRANYSQYAKKTK"
     gene            2499..>3286
                     /locus_tag="PLJ02_02980"
     CDS             2499..>3286
                     /locus_tag="PLJ02_02980"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOI05404.1"
                     /translation="MTDNFDQVLANFHYDLPKSKIANQPLPERDQSKLLVFDRQTGQF
                     SDYQFFQLDQLLTSQDVLVVNESKVFPARLLGKKNTGGKVELLLLKQISGDSWQAISK
                     PSLKLHQRLYFPPRNYLPDENLDLEDLLQAQVMARSDYSAQVEVKFNHADDSLWQAIE
                     ACGYTPLPPYIKNKQSEALRRKEYQTIYAKKVGSAAAPTAGLHFTEELLTKLKNKGVQ
                     IEKIDLAVGLGTFAKLTRKNWENQELHQEYFVIDEAVAKRLTEA"
BASE COUNT         1061 a          574 c          594 g         1057 t
ORIGIN      
        1 atagtgaatt ttttaatttt ttgtggatca acacaaatgc caatcggaat aatgggtgcg
       61 ccagtagcta aagccattct taccgcccca gtttttaact tttctacctg ttgactattt
      121 ttactaacca ttccttcagg aaaaattaaa attgagcgat tttttgtaag taaagtgatg
      181 gcttgattga gggcaattaa acctgaattt cggccaacag ctacatggcc agcccaagcg
      241 attaatttgt tcaggccaaa aattttaaac acatcttcgg tcaagagagc atgaaccggt
      301 tctttgatac tgctcatgat ataaagagga tcaagagtgc tcgggtgatt aaccgcaaaa
      361 atttttgctc ccaagggaaa ttgttttttt gatttaattt gtttttttaa atcaattttg
      421 gtgtaaagac gagcgattga ccgtcccaca aaatataaaa agttttctaa ataatagtgt
      481 tttttcattt ttttaagcag ctcattaagt cacatttaga ttagaaaagc aagcaatgag
      541 caaaaagtca ggtttatcgc tcttaattat ctgagttaag ggaatcttga ataaaatttt
      601 gcaactgggt ccagttgtta ggtccagcct taggaatgag aacaaagcca ttagaactac
      661 tactactatt caacacattt tccgtactga gattgatatt tttaatcgta aaatcattgg
      721 cattaagtaa taatggggcc aaatattcga gtatttctaa atttaaatca gtagtaacgt
      781 gctgattaac ttttttgaaa aaatctggaa tatgactgag agcttctagc tcaaacaact
      841 tttttctaat tccagtgagc acctcgactt ggcgccgact gcgatcgaaa tcactagaac
      901 tgtgtcggga acggacatat tttagcgcct cggctccttc cattttttgt aatccagctg
      961 gaaaataaat agtttcataa cgacaggtaa attgtttttc taattcaaaa ccactgagag
     1021 tggcagttag ttgagcaatt tcttcattac tataaccaca cgggtccagt tgcttgccct
     1081 caattggata ccattgatcc ttaaaatctt cggcaacttc tacttcaata ctgcctaatt
     1141 cttgaccaat taagcgttga aagccaacaa agtcaactgc aatgaaatat ttaatattta
     1201 aattggtgat agaagataag agatttttag taacttgacc agcattaaca attgggtctg
     1261 aaccagtcat ctgtgaacta aaaacattat taattttttg ataacgacca ttgcccaaac
     1321 tcaattccaa atctctgggt atagaaataa aagctagagt gtttttttca aaatcaaagt
     1381 gagcaatttg aatgagatca gccagcattc caccttgatg tcccgctcct ccgtagccaa
     1441 ttaggagaac attgagttct tttcgatcat tttcttcaat ctgactagta tctgaattta
     1501 acggatgagt agcttgtgcc tcaattaaaa tctcatcttg agtttgcttt tgttgttgaa
     1561 aatgctggta agcgacagtg gccgaaatgg agataattaa aattaataag cagagtaaaa
     1621 catataaaac agttttttgt tttttggtaa attttcgtaa ttttttagtt aaagtttgtc
     1681 taactttttt aaacattgat tatttagtat aactcaggtg atataataag actatgtcta
     1741 atcaatcctt ttttgatctt aaaaagaata attttcccta cgatcagtcc cgatttcctg
     1801 gccaagctag tgatgaaaga atactctatg ttaccagaga acacccgctt tttttgtttc
     1861 tgcgcctcgc ttttgttttg ttagtgggac tgagcattat attagctggt agcattttaa
     1921 atcggacact taatgcagtt atgacgaatc aactcttatc agaattaatt ttggtgatat
     1981 tagtgatttt tttcatattt atggttattg gagtttggtg ggtctttacc ttatggaaga
     2041 aatcagtagc aattgtgact aataaaaggc tgattaaatt tatttatacg acacctttta
     2101 atcgttataa catgagttta cctttggaga tgattgttga tactagtttt cacaatcgag
     2161 gttttttgag ctcctatctc aagcttggca gtattacggc tcgttcaagt gctagtagtt
     2221 cgggagtagc tacggacgat cctgcccgag ttaataaaaa atatttttat atcgaaaata
     2281 ttcagtatgc tgaggattta caacaatatt taaacaaatt aattaatacc ttgcataaag
     2341 aacccaagga acttgccaat ttccgacctt ttttacccaa cttaaaaggc gaggcaagag
     2401 aagatttctt gagagccaat tattcccaat acgccaagaa gactaaataa ttatggtcta
     2461 agcttggcga catattaagg gagtgttaaa ctagcagcat gactgataat tttgatcaag
     2521 ttttagccaa ttttcactat gatttgccca aaagcaaaat tgctaatcag cctctaccag
     2581 aaagagatca atctaaactt ttagtgtttg atcggcaaac tggtcagttt agcgattatc
     2641 aattttttca attagatcaa cttttaacca gccaagacgt tttggtagtt aatgaaagta
     2701 aagtttttcc agcccgcttg ttaggtaaaa aaaatacagg tggcaaagta gaactactgc
     2761 tgttaaaaca aatcagcggc gacagttggc aagcgattag taaaccatca ctaaaattgc
     2821 accagcgctt atactttcct ccacggaatt atttaccaga tgaaaattta gacctagaag
     2881 atttactgca agctcaagta atggctcgca gtgattattc tgctcaagtg gaagttaaat
     2941 ttaatcatgc tgacgatagc ttatggcaag ccattgaagc ttgtggctac acaccactgc
     3001 cgccatatat taaaaataag caaagtgaag cattgcgtcg caaagaatac caaactattt
     3061 atgccaaaaa agttggttca gctgctgcgc caacagccgg cttacatttt accgaagaac
     3121 tcttaacaaa gctaaaaaat aaaggcgtcc aaatcgaaaa aattgattta gcagttggtt
     3181 tgggcacttt tgctaaattg acaagaaaaa attgggaaaa ccaagaactt caccaagaat
     3241 attttgttat cgatgaggca gtggcgaagc gactcactga agcaag
//
LOCUS       DAOSEW010000071         3255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_27657_length_3255_cov_3.963437, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000071 DAOSEW010000000
VERSION     DAOSEW010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27657_length_3255_cov_3.963437"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..554
                     /gene="rplL"
                     /locus_tag="PLJ02_02985"
     CDS             153..554
                     /gene="rplL"
                     /locus_tag="PLJ02_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOI05405.1"
                     /translation="MAEEAKKLSAKVQKLVDAVKELSLMEVSELVSALEEEFGVSAAA
                     PMMMAAGPAAAAAPAVEEKDEFDVILKEAGNNKIAAIKAVRTLKPDLGLGDAKTFVES
                     APKTVLEGAKKEDAEAAKKALEEAGCVVELK"
     gene            945..1229
                     /locus_tag="PLJ02_02990"
     CDS             945..1229
                     /locus_tag="PLJ02_02990"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOI05406.1"
                     /translation="MSDSAVINPGSAESDEKKTGRATDAAKLDIKNLPDLLTVREVAD
                     LLRVSPLTIKRWGKRGKLPAIRINSRGDRRYKKEAVLWLLGVNPEELKEN"
     gene            complement(1246..2118)
                     /locus_tag="PLJ02_02995"
     CDS             complement(1246..2118)
                     /locus_tag="PLJ02_02995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HOI05407.1"
                     /translation="MKSLFFGLPIYTAGWSDFWVLLKNHLEAPQSRGLLLIFTPNPEI
                     LVQIEENPELKTVLESADILLPDGIGLLLANRWIRLRQRLLFLFKQPLMPAKIREKMA
                     GVELADKLLIEAAKKNYVSLIIGGRDYASFWGKQGGEYQGEIFEDEQSLIKLKKNLYW
                     TEAYQDKDEPLPIEDEAMERILKKIKPQIVFLALGSLDQEEWLVRWRARLEQAGVAVA
                     IGVGGAFDVLFGRVARAPGFFRSLGLEWLWRLIQQPWRWRRQLRLVKFLGLVNKEAKK
                     WQKMTQAQFVRKPS"
     gene            2190..>3255
                     /gene="murD"
                     /locus_tag="PLJ02_03000"
     CDS             2190..>3255
                     /gene="murD"
                     /locus_tag="PLJ02_03000"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOI05408.1"
                     /translation="MSDLAGSRIIILGLGREGLSSYRFLRTQLPEQELTLADQSPSEI
                     LFANFPEWQAISRQDRNLLWQLGDQYLENLANFDLIIKTAGIPVTLPAIQQALELNPG
                     LQISSNMQLFFDRCQGTIIGVTGSKGKSTTSQLIFDILSQAGQKTVLLGNIGRPALNY
                     LSVIDQETLVVAELSSHQLAELKASPQVAVLLDVTPEHLDYFANFEQYLAAKTAITRY
                     QSPNDWLTYNPALSGAKKLAQLSAVRPDHQIKHSLVDQSANLDFQVFIKDETIYYRES
                     NQPNQLRKLMAVNEIKLLGRHNLYNVLSAVAVAQIFQVKAASLRQTISNFQGLAHRLE
                     FVADVDGVQYFDDSIATNPTA"
BASE COUNT         1024 a          706 c          627 g          898 t
ORIGIN      
        1 ctcaagagcc caggtcaagg gttagtcaac accttaactg ctaagactaa agatttagtc
       61 tatgttctca aggcaattgc cgataaaaaa gagtaaaaaa attaaaaatt attaataatt
      121 agtcaaacca ctaattaaca gaaaggatca caatggcaga agaagcaaaa aaattgagcg
      181 ccaaagtgca gaaactagtt gatgcagtca aagaactttc cctaatggaa gtttcggaac
      241 tggtcagcgc cctcgaagaa gagttcggtg ttagtgccgc tgctccgatg atgatggccg
      301 ccggcccagc cgctgctgcc gctcccgctg ttgaggaaaa agacgaattt gacgtcattc
      361 tcaaagaagc tggcaacaac aaaattgctg ctatcaaagc cgtgcgcacc ctcaaaccag
      421 atctcggttt aggtgatgct aagaccttcg tggaatcagc tcccaaaaca gttttggaag
      481 gtgccaagaa ggaagacgct gaggccgcta aaaaagcttt agaagaagct ggctgtgtag
      541 ttgagctcaa ataagttaaa taagtaattt aacttactca agtttaactt actccaggca
      601 gatttcttaa agagtaatta aacattaacc ccgctcgtag cggggttttt gttagattgt
      661 ggttaaagaa tttttgacat attttgatct ttttcaataa attataggac taatttacta
      721 aagttagaaa agttatagga tattaaataa acaaagaagc taagatgcgc tcaaaatagc
      781 taaaaaaatc agtaatttgc ctaactttaa tcttgacata aaaattgctt attgacttaa
      841 tttgtcttag tctgctattg ttaagtatag atcataatgg atcgaacaat tactaaattt
      901 aacaactttt ataggcttaa aaaatcaagc cagagaaagg gaaaatgagc gatagtgcag
      961 tcataaatcc aggctccgcc gagagcgatg agaaaaaaac tggtcgggcc actgatgccg
     1021 ccaaattaga tattaaaaac ctccccgatc tactcactgt ccgagaagtg gctgatctgt
     1081 tgcgtgtttc cccactgacg attaagcgtt ggggtaaacg gggtaaatta cccgccattc
     1141 gcattaactc tcgtggtgat cgccgttaca aaaaagaggc cgttttgtgg ctgttaggcg
     1201 ttaatccaga ggaattaaaa gaaaactaga gcgttttaaa gataattagc tcggtttgcg
     1261 gacaaactga gcttgtgtca tcttttgcca ctttttggct tctttgttca ccaaacctaa
     1321 aaatttgact aaacgcaatt gccgtcgcca acgccacggt tgttgaatta agcgccacag
     1381 ccattccaaa cctaagctac gaaagaaacc aggcgcgcgc gccacgcgcc caaacaacac
     1441 gtcaaaagca ccccccaccc caatggccac cgccacgcct gcttgctcca agcgcgcgcg
     1501 ccagcgcacc aaccactctt cttgatccaa ggaaccgagt gctaaaaaaa caatttgtgg
     1561 tttaattttt ttgagaattc gctccatcgc ttcatcctca attggtaaag gttcatcttt
     1621 gtcttgataa gcctcagtcc aatacaaatt cttctttaac ttgatcaaac tttgttcgtc
     1681 ttcaaaaatt tcaccttgat attcaccacc ctgtttgccc caaaaactgg cgtaatcacg
     1741 gccaccaata attaaactta cgtagttttt tttggctgct tcgatcaata atttatcggc
     1801 cagctccacc ccagccattt tttctctaat tttggctggc attagcggtt gtttaaataa
     1861 aaacaacaac cgttgtcgca aacgaatcca gcgattagcc aacaacagtc caatcccatc
     1921 tggcaataaa atatcagcac tctctagcac tgtttttaac tcaggatttt cttcaatctg
     1981 caccaaaatt tctggattgg gcgtaaaaat gagcaataaa cctcttgatt ggggcgcttc
     2041 caagtggttt ttgagtaaca cccagaaatc tgaccaacca gccgtataaa ttggcaaacc
     2101 aaaaaataaa gacttcattt tttaatcata gcacaaagaa tgattgtgat cgcccaaaac
     2161 tagagtaaga atgctaaaat ggaggtacta tgtcagactt ggctggatcg cggattatta
     2221 ttttaggact gggtcgagaa ggtttgtcta gttatcggtt tttgcggacc caattgcccg
     2281 aacaagaatt gaccttagcc gatcaatcac ctagtgaaat tttatttgct aattttccag
     2341 aatggcaagc aattagtcgc caggaccgca atttactctg gcaacttggc gatcagtatc
     2401 tggaaaattt agccaatttt gatttaatta tcaaaacggc aggtattcca gtcactttac
     2461 ccgctattca acaagcgtta gaactcaatc ccggcctaca aattagttca aacatgcagt
     2521 tatttttcga tcgctgtcag ggcacaatta tcggtgttac tggtagtaag ggtaaaagca
     2581 ccaccagtca attaattttt gatattttga gtcaagctgg tcaaaaaact gtcttgctgg
     2641 gtaatattgg tcgtccagcg ctcaattatc tgtccgtcat tgatcaagaa accctggtgg
     2701 tggcagaatt gtcttctcat caactagctg aattaaaagc cagtcctcaa gtggcagtct
     2761 tactcgacgt cactccagag catctagatt actttgccaa ttttgaacaa tacttggctg
     2821 caaaaactgc tatcacccgc taccaaagcc ccaatgactg gttaacctat aatccagctc
     2881 ttagtggagc taaaaaatta gcgcaattaa gtgcagttcg acctgatcac caaattaaac
     2941 attcacttgt tgatcaaagc gctaacctag attttcaagt atttatcaaa gatgagacaa
     3001 tctattatcg agaaagcaat cagccaaacc aactacgtaa attaatggca gtgaatgaga
     3061 ttaaactgct aggcagacac aatctttata atgtcttaag tgctgtggcc gtcgcccaaa
     3121 tttttcaagt taaagctgct agcctacgcc aaactattag taatttccaa ggtttggctc
     3181 atcgtttaga atttgtggca gacgtagatg gagtgcaata ttttgatgac tcaattgcta
     3241 ccaacccaac tgcta
//
LOCUS       DAOSEW010000072         3252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_27700_length_3252_cov_4.053800, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000072 DAOSEW010000000
VERSION     DAOSEW010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27700_length_3252_cov_4.053800"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1166
                     /locus_tag="PLJ02_03005"
     CDS             <1..1166
                     /locus_tag="PLJ02_03005"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF020892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOI05409.1"
                     /translation="ELNLSNTHFIRTTNPKHTAAAQEFWRRCRANGDIYKANYPVKYC
                     VGCELAKTASELVDGCCPLHPDLALELREEENYFFKFSRYQKDLLKLYETKQDFVKPV
                     SRFNEIKAFVNNGLEDFSISRLKKNMSWGVPVPDDEEHVMYVWFDALVNYISTLGWPE
                     DLANFQQFWPAVQVAGKDNLRQQAAMWQAMLMSAGLPNSQQILINGFIGVDGQKMSKS
                     LGNVIKPKEMVERYGIDASRYLLIKLGTFGEDMDVSWEKFDQAYNASLANGLGNLCSR
                     LAKLANLNDLSINYQAKVPPQFKKLMDNYELTAALNLLDEGLGQIDQYLAKEKPWLRE
                     NHNHQEVITQAICSLLDVNQAIQIFMPETAIAINKKFNKNKISPFTQGLFKRI"
     gene            1183..2052
                     /locus_tag="PLJ02_03010"
     CDS             1183..2052
                     /locus_tag="PLJ02_03010"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOI05410.1"
                     /translation="MPIFDTHCHYNLEPLYSGQAIYFDIDENDPILQKNWRDHWQTAQ
                     KNGIKQALIPGAGLTSSRLAVDIAQQEPNLYASVAIHPLRVAKIDFASATEEIEKLSQ
                     NQAVVAIGETGLDYFHLPKEDSEKVNNIKQDQERLFIKHIQLASQNQLTLIVHVRDEG
                     EAAYWRILEILDQYWPAEENIIFHCLSGPIAYVKKAMKIKHSYFGFDGNLTFKNAHYL
                     RELFQLLNQQSPDKILLETDAPYLAPTPYRGQICQPVMIGELANFVEKDLAGNLEQIY
                     QNSLRAFKLENNG"
     gene            2061..>3252
                     /locus_tag="PLJ02_03015"
     CDS             2061..>3252
                     /locus_tag="PLJ02_03015"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOI05411.1"
                     /translation="MIKRYFRRHPIRTKRALEILPGFTSWFLILFPIWGSFLVPKFVA
                     YYVIIFAVYWLYRSINLAIFAGIAQNKVRKNSQNDILPQLKKQFPKRWNTIHHLIIIP
                     TFQEPLGTLENTLTGLSKQSFPRQNIHVMLSFEEREGIEADKKAAYLQKKFAKVFGHL
                     WSTKHPDIKGEIKGKSSNTAWGAKRAKQLLVDENGINIDNVTITSEDADAVLHPYYFS
                     FLTYSFLTSDKPYNKSWQAAICFYNNIWQVPAPIRVLAATQSVTQIYILARRDRLINF
                     STYSVSLKHIEDIGYWDTDVIPEDYRLFFKSYFAKGGDFEVEPIFLPIYADAAQSTGF
                     WSSMRNQYEQIKRWAWGVSDDAYIIRHYVLNDNAPFIDKTLRVLHIIEDHFLWPVNWF
                     AVTVS"
BASE COUNT         1035 a          611 c          609 g          997 t
ORIGIN      
        1 atgaattaaa tttgagtaac actcatttta tccgcaccac caatcctaaa catactgctg
       61 ctgctcaaga attttggcga cgttgccgag caaatggcga tatttataaa gctaattatc
      121 cagttaaata ttgtgttggc tgtgaattag ctaaaactgc tagtgaattg gtcgatggtt
      181 gttgccccct tcaccccgat ttagcattag aattgcgaga agaagaaaac tattttttca
      241 aatttagtcg ttatcaaaaa gatttattaa aactttatga aaccaagcag gattttgtta
      301 aaccggtcag ccgctttaat gaaattaaag cttttgttaa caacggttta gaagatttta
      361 gtatttcccg cctcaaaaag aatatgtctt ggggcgtacc cgtacctgat gatgaagaac
      421 atgtgatgta cgtctggttt gatgctttgg tcaattacat ctcgaccctg ggttggccag
      481 aggacttagc caattttcaa caattttggc cagcagtcca agtggctggt aaagacaacc
      541 tgcgtcaaca agctgccatg tggcaggcca tgctcatgtc ggctggttta cccaactctc
      601 aacaaatttt gatcaatggt tttattggtg tcgatggcca aaaaatgagc aaatctctcg
      661 gcaatgtgat taaacccaag gaaatggtag agcgttatgg cattgacgct agtcgttatt
      721 tactgatcaa actcggaact tttggcgaag acatggatgt tagttgggaa aaatttgatc
      781 aggcttataa tgcttcttta gctaatggac taggcaattt atgttctcgt ttggcaaaat
      841 tggctaattt aaatgattta tcaattaatt atcaggccaa agttccgcca cagttcaaaa
      901 aattgatgga taattatgag ctaactgccg ccttaaatct attagatgaa ggtctcgggc
      961 aaatagatca atacttggct aaagaaaaac cttggctcag ggaaaatcat aatcatcagg
     1021 aggtaataac tcaagctatt tgttcactat tagatgttaa tcaggccatc caaattttta
     1081 tgccagagac tgcgatagct atcaacaaaa agtttaataa aaataaaatt agtcctttta
     1141 cccagggtct atttaaacga atttaacaaa agaataataa tcatgccaat ttttgatacc
     1201 cattgtcatt ataatttgga acctctttat agcgggcagg caatttattt tgacattgac
     1261 gaaaatgatc caattttaca aaaaaactgg cgagatcact ggcaaaccgc gcaaaaaaat
     1321 ggcattaaac aggcccttat tcctggggct ggattaacca gtagtcgctt agctgttgat
     1381 attgcccaac aagaaccaaa tctctatgca agtgtggcca ttcacccctt acgcgtggca
     1441 aagattgatt ttgcttcagc gactgaagag attgaaaaat tgagtcaaaa ccaagctgta
     1501 gtcgctattg gcgaaactgg tctcgattac tttcatttgc cgaaagaaga ttcagaaaaa
     1561 gttaataata ttaaacaaga tcaagaacga ttatttatca aacacattca actagcgagt
     1621 caaaaccaat taaccctgat tgttcatgtt cgagatgagg gtgaagcagc ttattggcgt
     1681 attttggaaa ttttagatca atattggcca gctgaggaaa atattatttt tcactgcctt
     1741 tctggaccga tagcttacgt gaaaaaagcc atgaaaatta aacatagtta ttttggcttt
     1801 gatggtaatt taactttcaa aaatgctcat tatttaagag aactttttca attgttaaat
     1861 cagcaatcac cagacaaaat tttactagaa actgatgctc cctaccttgc ccctacacct
     1921 tatcgtggcc aaatttgtca accggttatg ataggtgaat tagctaactt tgtggaaaaa
     1981 gatttggcag gcaacttaga acaaatctat caaaattcgc taagggcttt taaattagaa
     2041 aataatggat aaattaatta atgattaaac gctactttcg tcgccaccct atccgcacca
     2101 aacgggcatt ggaaattttg ccaggattta cttcttggtt tttaatttta tttcctattt
     2161 ggggttcttt tttagtgcca aaattcgttg cttactacgt aattattttc gccgtctatt
     2221 ggctgtacag atcaattaat ttggccattt ttgctggtat cgcgcaaaac aaagtcagaa
     2281 aaaattccca aaatgatatt ttgcctcaat taaaaaaaca atttcccaaa cggtggaata
     2341 caattcacca cttaattatt attccgactt ttcaagaacc tctgggcacg ctagaaaata
     2401 ctttgactgg actaagtaaa caaagttttc cccgtcaaaa tattcacgtt atgctttctt
     2461 ttgaagaaag agaaggaata gaagcagata aaaaagctgc atatttacag aaaaaatttg
     2521 ctaaagtttt tggtcatctc tggtcgacta aacaccctga tatcaaaggc gaaattaaag
     2581 gcaagtcatc aaatacggct tggggagcaa agagggccaa acaactgcta gtggatgaaa
     2641 atggcattaa cattgacaac gtcactatta ccagcgaaga cgcagatgca gttttacacc
     2701 cctattattt ttcattttta acatatagct ttttgactag tgacaaacct tataacaaaa
     2761 gttggcaggc ggcgatttgt ttttacaata acatctggca agtgccagcc ccaatccggg
     2821 tgttggcagc tacgcaatcc gttactcaaa tttatatttt agcgagacgt gatcgtttaa
     2881 ttaatttttc cacttattca gtttccttaa aacacattga agatattggc tattgggata
     2941 ctgatgttat cccagaagat tatcgactgt tctttaaatc ctattttgcc aaaggtggcg
     3001 attttgaagt ggagccaatc ttcttaccga tttacgctga tgctgcccaa tcaaccggtt
     3061 tttggagcag tatgcgcaat caatatgaac aaattaaacg ctgggcttgg ggagtgagtg
     3121 atgatgccta tattatccgc cactacgtgc tcaatgataa tgcccccttc atcgataaaa
     3181 ccctcagagt tctacacatc attgaagatc attttttatg gccagttaac tggtttgccg
     3241 tcactgtctc ag
//
LOCUS       DAOSEW010000073         3210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_28233_length_3210_cov_4.063074, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000073 DAOSEW010000000
VERSION     DAOSEW010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28233_length_3210_cov_4.063074"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="PLJ02_03020"
     CDS             complement(<1..686)
                     /locus_tag="PLJ02_03020"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOI05412.1"
                     /translation="MSELNSDEILNQELDELRAKIKQVQLPEELQSKLEKELVFLERS
                     AKLGNYDEKYDKVSRYLDWVTKIPFGKKSEDTLDIQQAKVVLDEHHYGLKEVKDRFLE
                     YLAILNLQKQNFADQEFSAPVLLLVGLVGTGKTTFAYSLAEALNRQILRIPVGGMASA
                     KELRGESRLILEAQPGAVIQGLCTAQTMNPVILLDEIDRSAEEANNDIMGVLVELLDP
                     AQNHAFVDNY"
     gene            complement(694..1203)
                     /locus_tag="PLJ02_03025"
     CDS             complement(694..1203)
                     /locus_tag="PLJ02_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05413.1"
                     /translation="MKKIVICLISLLIIFPLFFSSKVRAAALQITAIGTMDVSNLDLG
                     GSLKEYNYSGGTFALTGLASPSAVISITIDDLTRTATADEEGSWSTLISSLSEGEHQF
                     DLSTATETLDFVLTIGNSSTASGNLETVATTSTTTLPNAGSTTVSLLFLAVAMLSIGL
                     GLTLRAKQS"
     gene            complement(1227..2165)
                     /locus_tag="PLJ02_03030"
     CDS             complement(1227..2165)
                     /locus_tag="PLJ02_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOI05414.1"
                     /translation="MYNCISIGSSLVDIFISSKDWEAKRNKNQQILTYGDKLDLSDFH
                     IFTGGGASNTAVALSRLGFKVAVISETGQDEFSDLIIRDFEKEGVATQLLVREKLERT
                     GGSVVLVTKDSERIVLTYRGAASMLDPYDIPAFWLTQANWVHLSSIAGQQATLTKIFA
                     TLLKDQDTKLSWNPGKAEIDLLVKRTFDFNDIPCEVFFVNQQEWTLLAPVQKEILANF
                     PQVIVTMGKKGGEVYLYGKKKFVFEGQGDHTVDDTGAGDAFSSAYIAALIDNREIEEA
                     IVWGVRNASNVVRYYGAKTGLLRRYQMLELLAAQKK"
     gene            complement(2277..2921)
                     /locus_tag="PLJ02_03035"
     CDS             complement(2277..2921)
                     /locus_tag="PLJ02_03035"
                     /inference="COORDINATES: protein motif:HMM:NF015597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HOI05415.1"
                     /translation="MTHHEQKKINVTKANPKANPKVKLLTRIDRVAFYGSADIAEDHP
                     VCRAAYEVAAYVASQNKIVVNGGGPGVMGAATRGAKSKNGKTLAVTFRPVDMPEFEGR
                     DAKNVVDQEIKTANYLERMAGLMDNADLFVCFQGGTGTLSEWATAWLLAHLHYGNHKP
                     LILYGHFWYEVMEVIHKNFLIGYKESKVYRIVDNLEEFKVALHLFEDEMRERQK"
     gene            complement(2918..>3210)
                     /locus_tag="PLJ02_03040"
     CDS             complement(2918..>3210)
                     /locus_tag="PLJ02_03040"
                     /inference="COORDINATES: protein motif:HMM:NF013030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOI05416.1"
                     /translation="IEKHAWEYLYQVLLMAVEAGAQGQTFNSLIFAKIEELKNKINQS
                     NKPIKIVVDGGIKQEQIKDLQKAGVDQIAVGSAVFDADNFKTEFNKLKKELQ"
BASE COUNT         1015 a          715 c          489 g          991 t
ORIGIN      
        1 atgtaattat cgacaaaagc atgattttgc gcaggatcta gcagttcaac cagaacaccc
       61 ataatatcat tattggcttc ttccgcggat cgatcgattt catcaagtaa aatcactgga
      121 ttcatcgttt gggcagtaca aagaccctga attaccgccc caggttgagc ctccaaaatt
      181 aagcgtgatt caccacgcaa ttctttggca gaagccattc ctccaaccgg aattcttaaa
      241 atttggcggt tcagggcttc cgctaaagaa tatgcaaaag tagttttacc tgttcctacc
      301 aaaccaacta gcaataatac tggcgcagaa aattcctgat cagcaaaatt ttgtttttgt
      361 aaattaagaa tagctaaata ctccaaaaat cgatctttaa cctcttttaa accataatga
      421 tgttcatcaa gaactacttt agcctgttga atgtctaaag tgtcttcact ttttttacca
      481 aatgggatct tggttaccca gtctaagtat cgagaaactt tatcatattt ctcgtcataa
      541 ttgcccaatt ttgcactccg ctccaaaaaa actaattctt tttccagctt tgattgtaat
      601 tcttcgggaa gttgaacttg tttaatttta gctcgtaact catcaagctc ctgattcaat
      661 atttcatcag agttaagttc agacataagt aaattaagat tgttttgccc taagcgtcaa
      721 gcctaaaccg atacttaaca tcgcaacagc taaaaataac aaactaacgg tggtagaacc
      781 agcattaggc agggtagtag tgctagttgt agcaacagtt tcaagattgc cactggcagt
      841 actactgtta ccaattgtta acacaaaatc taaagtctct gttgctgtag ataaatcaaa
      901 ttgatgctct ccttcactta aagacgagat tagcgtactc caactaccct cctcatctgc
      961 agtcgccgtt ctagttaagt catcaatagt aattgaaata acggccgagg gtgaagctaa
     1021 accagttaag gcaaaagtcc ccccagaata attatattcc ttcaaactcc cacccaaatc
     1081 taaattagaa acatccatgg tacctatggc tgtaatttgc agcgcggccg cccttacttt
     1141 actactaaaa aagagtggga aaataatcaa taaagagata agacaaatta caattttttt
     1201 catatttctc atctagtcta acatttttac tttttttgtg cagctagcaa ttctaacatt
     1261 tgataacgac gtaataaacc agttttagcc ccataatatc tcactacgtt actagcgtta
     1321 cgaactcccc aaacaatagc ctcctcaatt tctcgattat cgatcagtgc tgcaatatag
     1381 gccgaggaaa aagcatcacc agcaccagta tcatcaacag tatgatcgcc ctgaccttca
     1441 aagacaaatt tcttttttcc atacaaataa acttcaccac cctttttccc catggtaaca
     1501 attacttgag gaaaattagc taaaatttct ttttgcactg gagccaacaa agtccactct
     1561 tgttgattaa caaaaaagac ttcacaagga atgtcgttaa aatcaaatgt tcttttcact
     1621 aataaatcaa tctcggcttt gcctggattc caagataatt ttgtatcttg atctttaagc
     1681 aaagtggcaa aaattttagt caaagtagcc tgctgaccag ctatacttga tagatgaacc
     1741 caattagctt gagtcagcca gaacgcagga atatcgtaag ggtcgagcat cgatgccgct
     1801 ccgcgataag tcaaaacaat tctttcacta tctttcgtaa ccaacaccac actgccaccc
     1861 gtacgctcta atttttctct aactagtagt tgggtggcca ctccttcttt ttcgaaatct
     1921 cgaataatca aatctgaaaa ttcgtcttga ccagtttctg aaatcactgc tactttgaaa
     1981 cctaaacgac tgagagcaac agcagtatta ctggctccac ctccggtaaa aatatgaaaa
     2041 tcacttaagt ctaacttatc tccataggtc agaatctgct gatttttatt acgtttagct
     2101 tcccaatctt tggaactaat aaaaatgtcg actagtgaag atccaatact aatgcagtta
     2161 tacataaggc aggttctttc attgtacaag atactaaaca acaattgcaa gaatggagat
     2221 aaaaaatagt gataatgagt aaaaacaagc aaaccattgc cgactcagaa tattttttat
     2281 ttttgacgtt cccgcatttc atcctcaaat aagtgcagtg ctactttaaa ttcctccaaa
     2341 ttatccacaa tccgataaac ctttgattct ttatagccaa ttaaaaaatt cttgtgaata
     2401 acctccatga cctcatacca aaaatggcca tacaagatca gtggtttgtg attgccatag
     2461 tggagatgag ccaaaagcca agccgttgcc cattcgctta acgtaccagt cccaccctga
     2521 aaacaaacaa aaagatcagc attgtccatc aaaccagcca ttcgttccag ataattggca
     2581 gttttaattt cttgatctac aacatttttt gcatcacgtc cttcaaattc tggcatatcg
     2641 actggcctaa aagtcacggc taaagtttta ccattttttg atttagctcc cctggttgca
     2701 gctcccatta ctcccggccc accgccatta accactattt tattttgact agcaacataa
     2761 gctgccacct catacgcagc ccgacacact ggatgatctt ctgctatatc tgcactgcca
     2821 taaaaagcaa ctctatctat cctggttaaa agcttgacct tgggattagc tttgggattg
     2881 gctttggtaa cattaatttt tttttgctca tgatgagtca ttgtaattcc ttttttaact
     2941 tattaaattc agttttaaaa ttatctgcgt caaagacagc tgaaccaaca gctatttgat
     3001 caacaccagc tttttgtaaa tctttaatct gttcctgctt aatgccacca tcaaccacta
     3061 ttttaattgg tttattagat tggttaattt tgttttttaa ttcttcaatt ttagcaaaaa
     3121 ttaaagaatt aaaagtttga ccttgtgctc cggcctcaac agccattaat aagacttgat
     3181 acaagtactc ccaggcatgt ttttcaatat
//
LOCUS       DAOSEW010000074         3027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_30667_length_3027_cov_4.537685, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000074 DAOSEW010000000
VERSION     DAOSEW010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30667_length_3027_cov_4.537685"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..116)
                     /locus_tag="PLJ02_03045"
     tRNA            complement(48..116)
                     /locus_tag="PLJ02_03045"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(84..86),aa:Ile,seq:cat)
     gene            214..287
                     /locus_tag="PLJ02_03050"
     tRNA            214..287
                     /locus_tag="PLJ02_03050"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:248..250,aa:Met,seq:cat)
     gene            complement(348..1979)
                     /gene="groL"
                     /locus_tag="PLJ02_03055"
     CDS             complement(348..1979)
                     /gene="groL"
                     /locus_tag="PLJ02_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541697.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOI05417.1"
                     /translation="MAAKQLLFGDSARQKMLIGINKLAEAVTTTLGPKGRNVALDRSW
                     GAPQVIHDGVSVAKEIELEDKFENMGAQLVKEAADKTNEAAGDGTTTSTLLAQAIANK
                     GMKYVTAGANPMIMKRGIDKAVSAVVKEIRRLSKDVTEADWQKVATISAQDEVIGEKI
                     AEALKLVGKDGVVEVEEGKTMEITIEHKEGMEFDKGYASPYFVTDSDSMEAVSDKPYI
                     LVTDQKISNIKDILPLLEAVVNANRELVIIADDLEGEALTTLVVNKLRGTFKCLPIKA
                     PGFGDRRKAMLQDIAILTGANLISEDTGRKLADATIDDLGQADSVRSNKDSTRIVGGK
                     GKQADIDARVAQIEHEIANSTSDFDIEKLEERRAKLTGGVAVIQVGAATEIEMKDLQE
                     RVKDAKEATKAAIEEGIIAGGGVTLIRAGKVLAEVEKKAKTEDEKTGVRLIAEILEEP
                     LRMLATNSGEDAGWVVGKVKDKNQANWGFNALTNEFEDLLAAGVIEPAKVAIASLVNA
                     AGVASMILTTECLVADAPKKEEPALPAGGGMPGMM"
     gene            complement(2032..2334)
                     /locus_tag="PLJ02_03060"
     CDS             complement(2032..2334)
                     /locus_tag="PLJ02_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016478011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOI05418.1"
                     /translation="MSKLDVKKLKPTAGYILVKPAEKEKQTASGIILAVDDSEKPQYG
                     EVLAVGASIFDDGQEIKAPATVGDQVVYKKWGGNDVTIDDIEYQFLKFEDILAIIK"
     gene            complement(2483..2908)
                     /locus_tag="PLJ02_03065"
     CDS             complement(2483..2908)
                     /locus_tag="PLJ02_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05419.1"
                     /translation="MAKNDYLRELLNEIKLRYHLGEEVFADSSQPLTSSSQLEENSNS
                     RQLGFTLELKIIELKQLNNLLEKVAVKSPKQRAWWLDHHQEFVEQISQLLINYSNLSL
                     LAFKGGNTNTRLSLILAEEIEKAISLMNTTFFDDFVINA"
BASE COUNT          838 a          698 c          521 g          970 t
ORIGIN      
        1 gtcaatcaag ctttaatgct atttttgatc ccgctattgc cgtattgttg cgggactagg
       61 agtcgaacct agcctgtggg attatgagcc ccacgtgcac cgtacactat cccgcgtttt
      121 gatgctcgct attatagcct aagcgctgct tgtttttcaa gagaaaaaat cttaaaatat
      181 cccagattaa agaatcaaca aagagagagt tgcggcgagg tagctcagtt ggttagagca
      241 tcggattcat aaaccgaagg tcgtgagttc aactctcacc ctcgctacaa agaaactaaa
      301 aaaggaggca tggtgcctcc tttttttatc agcggtaaca atgtctttta catcattcct
      361 ggcataccac ctccggctgg taaggctggt tcttctttct taggagcatc agccaccaag
      421 cattcagtag tgagaatcat tgaagcgact ccagcagcgt tgactaacga agcaatcgct
      481 actttcgctg gttcaatcac tccagcagcc aacaagtctt caaactcatt ggtcagagca
      541 ttaaagcccc aattggcttg attcttatct ttaactttcc ccactaccca acctgcatct
      601 tctccagagt tggtggccaa catgcgcaga ggttcctcta aaatttcagc tatcagtcta
      661 actccggttt tttcatcctc agttttcgct tttttttcaa cttcagctaa aactttacca
      721 gctcgtatca aagtgacgcc gccaccagcg ataatacctt cttcaatagc agccttagtg
      781 gcttccttag catccttcac ccgctcctgc agatctttca tttcgatttc agtggcagct
      841 ccaacttgaa tgaccgctac gccaccagtt aatttggctc ggcgctcctc taatttttca
      901 atgtcaaagt cagaggttga attggcaatt tcatgctcaa tctgagctac ccgagcatcg
      961 atatcagcct gtttgccttt gccaccaaca attctggtgc tgtctttatt agaacgcacg
     1021 ctatctgctt gacccaaatc atcaatggta gcatccgcta atttacgacc ggtgtcttca
     1081 ctgattaaat tagcacctgt caagatagca atatcttgca acatggcttt gcgacgatca
     1141 ccaaatcctg gagctttgat tggtagacac ttaaaggtac cacgcaattt gttaactacc
     1201 aatgtcgtta aagcttcacc ctctagatca tcggcaataa tcaccagttc gcgattggca
     1261 tttaccactg cttccaacaa gggcaaaatg tctttaatat tactgatttt ttgatcagtg
     1321 accaaaatat atggtttgtc gctaactgct tccatgctgt cgctatcagt cacaaaatat
     1381 ggagaagcat agcctttatc aaactccata ccttctttgt gctctatggt gatttccatg
     1441 gttttacctt cttccacttc gaccacgcca tctttaccca ctaatttcag tgcttcagca
     1501 attttctcac cgatgacttc atcttgtgca gaaatagtgg ccactttttg ccagtcagct
     1561 tcggtcacat ccttagataa acgacgaatt tctttgacca cggcactcac tgccttatca
     1621 attcctcgtt tcataatcat tggattagcc ccagcagtga catatttcat ccctttattg
     1681 gcaatggctt gagctaataa agtagaagtg gttgtgccat caccagcggc ttcattggtt
     1741 ttatccgctg cttctttaac taattgagca cccatatttt caaatttatc ctcaagctca
     1801 atttccttag ccacactcac tccatcgtga atcacttgtg gagcacccca actacgatcc
     1861 agggctacat tgcgtccctt gggacctaaa gtggtagtga ccgcttcggc taatttatta
     1921 atcccaatta acattttttg tctggcgcta tcgccaaata agagttgttt cgctgccata
     1981 attttccttc tttaaaaatt tttatatttt tatatttatt aataaaaatt attacttaat
     2041 aattgctaaa atatcttcaa atttgagaaa ttgatattca atatcgtcaa tggtgacatc
     2101 attgccaccc cattttttgt aaacgacttg atcaccaact gtggcagggg ctttgatttc
     2161 ctggccatca tcaaagatgc tagcccctac agccaaaact tcaccatatt gaggtttttc
     2221 gctatcgtca acagccaaaa taatgccgga agccgtttgt ttttcctttt cggcgggctt
     2281 gactaaaata taaccagcag tgggtttgag ttttttgacg tctaatttgg acataaaaaa
     2341 cctttctatt aatagcttct aatttttata attttaaatt cttgctttct cactaaactt
     2401 gttgagaaaa taattgctca atagagcgtc taaaagttat cacaaaattc gttttttagc
     2461 aagtgcttaa tttgactgct aattatgcat taatcacgaa atcatcaaaa aaagtagtat
     2521 tcatcaaact aattgccttt tcaatttctt cagctaagat caacgataag cgagtattgg
     2581 tattgcctcc tttaaacgcc agcagcgata aattggaata attaataagt aattggctaa
     2641 tttgctctac aaattcttga tgatggtcaa gccaccaggc tcgttgcttg ggcgatttta
     2701 cggcgacttt ttctaaaaga ttatttaatt gttttaattc aataattttt aattctaaag
     2761 taaaacctaa ttgtcgactg ttagaatttt cttcgagttg tgagctacta gttaggggtt
     2821 gtgaactgtc agcaaaaacc tcttcaccca agtgataacg taatttaatt tcattgagta
     2881 gctcgcgtaa ataatcgttt tttgccatat ttcaaggtat aataagcgcc ttaagaagca
     2941 ccatcataca agagaccatg caaattataa agttgaattt aaaacaagca acagatgccg
     3001 agctgactcg gctcatcgac caagcta
//
LOCUS       DAOSEW010000075         2898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_32683_length_2898_cov_4.066831, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000075 DAOSEW010000000
VERSION     DAOSEW010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32683_length_2898_cov_4.066831"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /gene="rpmE"
                     /locus_tag="PLJ02_03070"
     CDS             complement(<1..199)
                     /gene="rpmE"
                     /locus_tag="PLJ02_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012104031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOI05420.1"
                     /translation="MKKGIHPDWHHDSVVTCSCGNSFITGSTHKTLTVDICSACHPFF
                     TGEMKFVDRQGRVDKFIQKRTT"
     gene            complement(248..1498)
                     /gene="hisS"
                     /locus_tag="PLJ02_03075"
     CDS             complement(248..1498)
                     /gene="hisS"
                     /locus_tag="PLJ02_03075"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOI05421.1"
                     /translation="MQKNQELQTLKGFRDFLPAEALLREKVRSIIQNSFKRFAFVPFE
                     TPTLEYASLLLGKYGEEADKLLYTFTDRGGRQLGLRYDQTVPSARVLANYQNQLPKYL
                     RRYQIQTVFRADKPQRGRYREFVQCDADIFGSRSTLADAEILALFYNIFQDLGLSNIQ
                     IEYNDRQLLIDNLAEFANDQVSVLSIVQSIDKLDKFKPEIIELELVQKGLKDQKIEQI
                     LSKLNNIEKSAKLLDIEQKTLDLGVPQSMLRFNPKLARGLDYYTGLIFEAKTADFATN
                     NSLGGGGRYDNLIKELAGLTMPAVGFGLGFDRIVELIKEKNLIQTNDATQVLVAFFDQ
                     DKYQQTAIKLIKELRAAGINSEIYLEKDKINKQFKLAQDKNIPWVAVIGEEEFNKGLI
                     SLKNMATGQQDQLEIKQVIDKIQA"
     gene            1556..1708
                     /locus_tag="PLJ02_03080"
     CDS             1556..1708
                     /locus_tag="PLJ02_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05422.1"
                     /translation="MLLLIFSQQNIPFNQGDDERLIVEQLFFASGGYYMVFGSISQTN
                     ICYNLH"
     gene            1740..1816
                     /locus_tag="PLJ02_03085"
     tRNA            1740..1816
                     /locus_tag="PLJ02_03085"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1774..1776,aa:Ala,seq:cgc)
     gene            complement(1842..2573)
                     /locus_tag="PLJ02_03090"
     CDS             complement(1842..2573)
                     /locus_tag="PLJ02_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05423.1"
                     /translation="MSKLKIKSLNKKSFAQQSQYYLIIGLIIIVSFLFIKPLFSAQAQ
                     EDAQTEASEESTILNIKKVIQDKQVELGQGEQRTGQLKQAYLAQVKRVSAETITVLNR
                     NVNVVIPLTEDLEIMHNGEKIELDQIAVDDWIIVYSIIGADGVPTIKRLVLSDKDFTQ
                     TERQIVLGTITELYSTNLLFDSRTGANNLNYLLDVDTQYFDINGDEINQNDLYPQLQC
                     LIVAKIKNNGQWRASSIKALVDLND"
     gene            complement(2575..>2898)
                     /locus_tag="PLJ02_03095"
     CDS             complement(2575..>2898)
                     /locus_tag="PLJ02_03095"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05424.1"
                     /translation="IVDSQRLIVSGNTQANNFIVIFVNNEEIITNADDSGNFSKEVRL
                     DEGANVLVIYAINQDGLVSQNTRTVIVDADFLSEDFTNSTTAEEAIDDNQATAEADQE
                     KDTEE"
BASE COUNT          832 a          576 c          501 g          989 t
ORIGIN      
        1 ccgtggttcg cttttggata aatttatcaa ctcgtccctg acggtcaacg aacttcattt
       61 ctccggtgaa gaaaggatga caagcactac aaatatccac tgttagagtt ttgtgtgtag
      121 agccggtgat aaaactgttt ccacaactgc aagtgaccac actgtcatga tgccaatcgg
      181 gatgtatacc ttttttcata acgaacagca attataacat actttttttg gccagaaata
      241 gatgatttca agcttgaatc ttatcgatga cttgcttaat ctcgagttga tcttgttgcc
      301 cagtagccat attttttaag ctaattaaac ctttattaaa ctcttcttcg ccaatcactg
      361 ctacccaagg aatgttttta tcttgagcaa gcttgaattg cttgttgatt ttatcttttt
      421 ccaggtagat ttcactattg attccggctg ctcgcaactc tttaatcaat ttaatcgctg
      481 tttgttggta tttgtcttgg tcaaaaaaag ctaccagcac ttgagtagca tcattggttt
      541 ggattaagtt tttttcctta atcaattcaa caattcggtc aaaacccaac ccaaaaccca
      601 ctgccggcat tgttaatcca gccaattctt taattagatt gtcataacgt ccgcccccgc
      661 ccaaactgtt attagtagca aaatcagcag ttttagcttc aaaaattagg ccagtataat
      721 aatctaaacc tctcgccagt tttgggttaa atcttagcat ggattgaggt acccccaaat
      781 ccaaagtttt ttgttcaata tctaataatt ttgcagattt ttcaatgtta tttagtttgc
      841 tcaaaatctg ttcaattttt tgatccttta accccttttg gactaattcc agctcaatga
      901 tttcgggttt aaatttatct aatttatcta tactttggac aatggataga accgaaactt
      961 gatcgttagc aaattctgct aaattatcaa ttaataattg ccggtcatta tattcaatct
     1021 gaatattgct taaacctaaa tcttgaaaaa tattataaaa gagggctaag atctcggcat
     1081 cggctagagt agagcgactg ccaaaaatat cagcatcaca ttgcacaaac tcccgatagc
     1141 gacctcgttg cggtttgtcc gctcgaaaca cagtttggat ttggtaacga cgtaaatact
     1201 taggtagctg attttggtaa ttagctaaaa ctctggcact gggcaccgtt tgatcatagc
     1261 gcagacccag ctggcgtccg ccacgatcag taaaggtgta aagtaattta tccgcttcct
     1321 caccgtattt gcctaaaagt aagctggcat attctagagt aggagtttcg aaaggcacaa
     1381 aagcaaagcg cttaaaacta ttttgaataa ttgagcgtac tttttctctt agcaaagctt
     1441 ctgccggtaa aaaatctcgg aagcctttga gtgtttgtaa ttcttgattc ttttgcatat
     1501 atatccttta agtaccttta ttgtacaaaa aatccccatt attaggggat tgattatgct
     1561 attactcatt tttagtcagc aaaacatccc ctttaatcag ggtgatgatg agcgactaat
     1621 agttgagcaa ttgttttttg catcaggtgg ctattatatg gtttttggca gcattagtca
     1681 aacaaatatt tgttataatt tgcattaatt caatcttaaa ttgaaattac ttaaaaaacg
     1741 ggcctgtagc tcagttggct agagcgctgc attcgcattg cagaggtcag ggattcgact
     1801 tccctcaggt ccaccattta tctctcaggt tcactagttc actagtcatt gagatcaacc
     1861 aaagccttaa tgctactagc tcgccattga ccattatttt taatttttgc cacaatcaga
     1921 cattgtagtt gtgggtagag atcgttttga ttaatctcat cgccgttaat atcaaaatat
     1981 tgagtatcga cgtcaagcaa ataatttaaa ttatttgctc cagtccgcga atcaaaaagc
     2041 aaattagttg aataaagctc agtgatagta cccaggacaa tttgtcgctc agtttgcgtg
     2101 aaatctttgt cgctcagcac caaacgttta attgtgggaa ctccatctgc tccaataatg
     2161 ctataaacaa taatccaatc atcgacggca atttgatcaa gttctatctt ttctccattg
     2221 tgcatgattt ctaaatcttc tgttagggga atgaccacat taacatttct atttaggaca
     2281 gtgatagttt cggcgctgac tcgtttgact tgcgctaaat acgcctgttt gagctgtccc
     2341 gtgcgttgct caccttgacc tagttccact tgtttatctt gtataacttt tttaatattt
     2401 aaaatagtcg actcttcact ggcctcagtt tgtgcatctt cttgtgcttg cgcgctaaac
     2461 agcggcttga tgaataaaaa actaacaatg atgattaagc caattattag ataatattga
     2521 ctctgttgtg cgaaactttt tttatttaaa ctttttatct tcaactttga catattattc
     2581 ttcggtgtct ttttcttgat cagcttcagc tgttgcttgg ttatcatcta tcgcttcttc
     2641 agcagttgtg gaattggtaa aatcttcact caaaaaatca gcatcaacaa ttacggttcg
     2701 tgtgttttgg gaaactaagc cgtcttgatt aatggcatag ataaccaaaa cattggctcc
     2761 ttcatcgaga cgaacttctt tagaaaaatt accagaatca tcggcattgg taatgatttc
     2821 ttcattattt acaaaaataa ctatgaagtt atttgcttgc gtattgccac tgacaatcaa
     2881 acgctgacta tcaacgat
//
LOCUS       DAOSEW010000076         2788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_34582_length_2788_cov_2.954263, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000076 DAOSEW010000000
VERSION     DAOSEW010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34582_length_2788_cov_2.954263"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1459
                     /locus_tag="PLJ02_03100"
     CDS             <1..1459
                     /locus_tag="PLJ02_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05425.1"
                     /translation="NSYSFSWSDQAFRDFSNYLNVDQDQPLAKPEKIDDIYPFPTLFT
                     NHLQKDLEFSLSEDESYFYLTSLKTSPTVAYQLYYQDLLKAENNLPFRLSWVLADNTV
                     QLNFRLLAPAIIDRQQIINFSLQKTFNFDATLCLASASCYLNVNHQLITPVTESGEID
                     LLLDTSLVNTIALSTDQKTAYFAYDFFDLNLSNLESDIKDFAGGKQLTVKMPKVLLQS
                     NIFNTALNTTEAKDCRPLQDGLVVKERRPEGTNYQAVATSVCDHFYLSDLDHRLGYLI
                     KISAQNLSSLPAIFAVQVDSLGRSPLETYLSDGINYQVLPPTENFNQGYTLYFSTDSY
                     GKEINRNLLESTEVFYWPYAFIKSLRWQNSDQVLAKSQPLVCDFNVKKKALWFYQVDL
                     PANCDAKYISLAQAYDQGWLAVQEGKQLEQLKLNNWANAWLMQDEFATGSIYIFYWPQ
                     LLEYLGLLSIFLLIVFLLFKYRRSQSDRLQKKRSK"
     gene            1477..>2788
                     /locus_tag="PLJ02_03105"
     CDS             1477..>2788
                     /locus_tag="PLJ02_03105"
                     /inference="COORDINATES: protein motif:HMM:NF014581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HOI05426.1"
                     /translation="MPKNHLLLKIKTSKDSLETEEAAVQLFSTLPKLKNSLFNQLKGD
                     HESLSFEILVKEQEIHFLVSVPERLLEYFKGAIQASYPEAVIEKVNVDPVDHFLLKDR
                     QLALASLKLKNHDCLPLKTYQDFQDIDPLSTLMSTLSKNEGDDTILIQLLLANNFGLW
                     PANHGLSAEQLAEHPQQALIKLKSEQRQLKAAFKIAVATDNHQRSKLLLSNIAAAYQA
                     TSKSESNELLLKKYHLWKNLFLKTMKQRSFNLSPTLSLSVDELATLYHFPSQKLSNIK
                     NISWGKRILGEAPENLPIVSAETAEEIKEQINIIGETVYKNHEVKYGLLNADRRRHVY
                     VIGKTGTGKSTLLANMAINDLKHNKGLCVIDPHGDLVETLLDFIPKHRINDVVYFDPA
                     DTERTVQINLFEGENVVHRELIASGIISVFKKLYGYSWGPRLEYI"
BASE COUNT          890 a          481 c          511 g          906 t
ORIGIN      
        1 taacagttat agtttttctt ggtcagatca agcttttaga gatttttcaa attatttaaa
       61 tgttgatcag gatcagccat tagccaagcc agaaaaaata gatgatatct atccgtttcc
      121 cacgcttttt actaaccatt tgcaaaaaga tttagagttt tctttaagtg aagatgagag
      181 ctatttttat ctaactagtt taaagactag tccaacagtg gcctatcaac tttattatca
      241 agacttatta aaggcagaaa ataacttgcc gtttcgctta tcctgggtgt tagcagataa
      301 tactgtgcaa ttgaattttc gcttgctagc tccagcaatt attgatcggc aacaaattat
      361 taatttttct ttgcaaaaaa cctttaattt tgatgccact ttatgtttgg ccagcgccag
      421 ctgttacctg aatgttaatc atcaactgat cacaccggtg actgaaagtg gagaaatcga
      481 tttattacta gatactagtt tggtcaatac gattgcttta agtaccgatc agaaaaccgc
      541 atattttgcc tatgactttt ttgatcttaa tttatctaac ttagaaagtg atatcaaaga
      601 ttttgctggt ggtaagcaac ttacagtcaa aatgcctaaa gtgttactgc aaagtaatat
      661 ttttaataca gcactgaata cgacagaagc caaagattgc cgacctctac aagatggttt
      721 ggtggtcaaa gagcgacgtc ctgaaggtac taattatcaa gctgtggcta ccagtgtttg
      781 cgatcatttt tacttaagtg atttagatca tcgtctagga tatttaatta aaatttcagc
      841 gcaaaatttg tccagtcttc ctgctatttt tgccgtgcaa gtggatagtt tggggcgtag
      901 tccactagaa acttatttaa gtgatgggat taattatcag gtgttaccac ctacggaaaa
      961 ttttaatcaa ggttatactt tatatttttc gaccgattca tatggcaaag aaattaatag
     1021 aaatttgctt gaatctactg aagttttcta ttggccttat gcctttatca aaagtttacg
     1081 ctggcaaaac tctgatcagg tattagccaa aagtcagcca cttgtttgcg atttcaatgt
     1141 taagaaaaaa gctctatggt tttatcaagt cgatttacca gctaattgtg atgctaaata
     1201 cattagtttg gctcaagctt atgatcaggg atggttagct gttcaggaag gaaagcaact
     1261 tgaacagctg aaacttaaca attgggccaa tgcgtggttg atgcaagatg aattcgcgac
     1321 aggtagtatc tatatttttt actggccaca actcttagag tatctgggtt tgctcagtat
     1381 ttttttgctt atagtgtttc ttttatttaa atatcgtaga agtcaatcgg atcgtttgca
     1441 gaaaaaaaga tccaagtaat ttgttatact gagattgtgc caaaaaacca cctattgtta
     1501 aaaattaaaa ctagcaaaga tagtctggaa acggaagaag ctgcagtgca gctcttttcc
     1561 actttgccta aattaaaaaa tagtcttttt aaccaattaa aaggcgatca cgaaagtctg
     1621 agttttgaaa ttttggttaa agagcaagaa attcattttt tagtgtctgt gccagagcga
     1681 ctcctagagt attttaaggg agccattcaa gctagttatc cagaagcagt tattgaaaaa
     1741 gtcaatgttg atcctgtcga ccatttttta ctaaaagatc gtcagttagc cttagctagt
     1801 ctcaaactaa aaaatcatga ttgtttacct ctcaaaactt atcaggattt tcaagatatt
     1861 gatcctttat cgactctgat gtcaacttta tccaaaaatg aaggagacga taccatttta
     1921 attcaactgt tactggcaaa taattttggg ttgtggccag ctaatcatgg tttatccgcc
     1981 gaacaattgg ctgagcaccc gcagcaagcc ttaattaaac taaaatcaga acaacgtcaa
     2041 ctcaaagccg cttttaaaat tgctgtagct actgacaatc accaaagaag caaactccta
     2101 ctcagcaata tcgctgctgc ttatcaggct acttccaaaa gtgagagtaa tgaattatta
     2161 ctcaaaaaat accatctttg gaaaaatttg tttctcaaaa ctatgaaaca acgtagcttt
     2221 aatctttcgc ccactttaag tttgagcgtt gacgaattag ctacccttta tcatttcccc
     2281 agtcaaaaat taagcaatat taaaaatatt tcttggggca aaagaatcct aggtgaggct
     2341 ccagaaaatc taccgatagt tagtgctgag actgctgaag aaataaaaga gcaaattaat
     2401 attattggag aaacagttta taaaaatcat gaggttaaat atggcttatt aaacgctgat
     2461 cgacgccgtc atgtttatgt aattggtaaa actggcactg gtaaatccac tttattagcc
     2521 aatatggcca ttaacgactt gaaacacaat aagggtttat gtgtcattga tcctcatggt
     2581 gacctagttg aaactctatt agattttatc ccgaagcatc gcatcaacga tgtggtttat
     2641 ttcgatccag cagatactga acgcactgtg caaattaatc tgtttgaggg agaaaatgtg
     2701 gttcatcgag aattgatcgc ttcaggaatt atttctgtat ttaaaaagct ttatggttat
     2761 tcttgggggc caagactgga gtatattt
//
LOCUS       DAOSEW010000077         2760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_35100_length_2760_cov_4.062107, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000077 DAOSEW010000000
VERSION     DAOSEW010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35100_length_2760_cov_4.062107"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1800
                     /locus_tag="PLJ02_03110"
     CDS             <1..1800
                     /locus_tag="PLJ02_03110"
                     /inference="COORDINATES: protein motif:HMM:NF020076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HOI05427.1"
                     /translation="VDSEADGLLARITIEEEYQNQTNQEQEIIYEFNLPDEVAFYDLK
                     LGPNLEFSGVIAPKGAAQKVYERELVKRRDPALLEQTGPNQYRLRIFPVPGRNDFNTL
                     QGQRQKVSFSYSVAQNAGGFALPHYTRESNVYSDASSSISLAYNGQISYLQETSDQIA
                     AIDGEVRDLCLNQEQNLALNEAGRNANIIFHANDPDLQNLACAEIKDVLPLLKAYRLA
                     IVYDTSFNNEDDQTLDQFAKLLSQTDSGWLNEAQIDLYKFNQVISAGERITADNFKEL
                     LDPIHFSRVNDFSQLGKITGDYDLIVLVTSQDIDFTQLKNFPFFKPTMVYWVSEDQLP
                     ALNMEMTSGLWQTGGGTSTTIEEAIRSFLIKQELFTKYAENAINLNQKLSLQFSGLST
                     IPANNVFSANSALAMISNKALLQQFVSQQSKEVVGDINLLDQLDQAARQANLVSPYSS
                     LIALVNQQQIETLKRLMEQYNRFQDTQMIENESRINVFWDGPIPQPMPIFDDFSMEMI
                     PGSPMINKGVIGGEMMKQEAILNNDLLSLDKDVADGVTAQRRNAGGLGGFATGTGILS
                     NLSGGAAFIIGTGLIAVVGLVVSLLQQLKKKKK"
     gene            complement(1813..2400)
                     /locus_tag="PLJ02_03115"
     CDS             complement(1813..2400)
                     /locus_tag="PLJ02_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05428.1"
                     /translation="MPKTKKIASKKPTMNQTMKKSMSKTAKRPATKELDLSQEIMSKV
                     KTGEINMKPKWYFVLGSSLLFFAVVVLLVLAVFFFNLTVFMLRHQGPMRLWRLETLLS
                     QLPLWIPILAVLALWGGVKLLKKYDFSYKKNFLLIIVGLVLAIILAAFAIDSLGFNEF
                     WARKEPMRRFYQNLDPSLEGRHLRNTPRQGSGLQF"
     gene            complement(2379..>2760)
                     /locus_tag="PLJ02_03120"
     CDS             complement(2379..>2760)
                     /locus_tag="PLJ02_03120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOI05429.1"
                     /translation="FIKAFVNLQSFNIKKKFSSWLYRITHNEAMNRLNRHQREVPILK
                     DVDFASDLDLEDDFIKKELANLAKNCLAQMPIIYKEVLTLYYLEEQSYEAISDILRIA
                     TGTVGTRLNRAKIIMKKICQKQKK"
BASE COUNT          864 a          548 c          521 g          827 t
ORIGIN      
        1 gtagatagcg aagctgatgg tttattggct cggatcacta tagaggaaga ataccaaaat
       61 caaaccaacc aagagcaaga aatcatttat gaatttaatt tacccgatga agtagctttt
      121 tatgatttaa aactcggacc taatttggaa ttttctggag tgattgcccc caagggtgct
      181 gcgcaaaagg tttatgagcg cgaattggtg aaacgacgtg atccagcgct tttagagcaa
      241 actggaccca atcaatatcg cttaagaatt ttcccagtgc caggtcgcaa tgattttaat
      301 actttacaag gtcaaagaca aaaagtctca tttagttata gcgtggcaca aaatgcggga
      361 ggatttgccc taccgcacta cactcgagaa tcaaatgttt actctgatgc ttcaagctct
      421 atttccctcg cttataatgg ccaaatcagc tatttgcaag agactagcga tcaaatagcc
      481 gctatagatg gtgaagttcg agatctgtgc ttaaatcagg agcaaaacct agctctcaac
      541 gaagctggac gcaacgccaa cattattttt cacgccaatg atccagattt gcaaaactta
      601 gcttgcgcag aaattaaaga tgtattgccc ctgctcaaag cttatcgttt agccattgtt
      661 tatgatactt ctttcaataa cgaagatgac caaactcttg atcaattcgc caaattgctg
      721 agtcaaacag attcgggttg gttaaacgaa gcgcaaattg atctatataa gtttaatcaa
      781 gtaattagtg ctggagaaag aataactgct gataatttca aggaactact ggacccgatt
      841 cacttttcga gagtcaacga ctttagtcaa ttgggaaaaa tcactggtga ttatgattta
      901 attgttttag tcactagtca agacattgac tttactcaat taaagaattt tcccttcttt
      961 aagccgacca tggtttactg ggttagtgag gatcaattgc cagcactgaa tatggaaatg
     1021 accagtggac tctggcaaac cggtggtggc actagcacca ccattgaaga agcgattcgt
     1081 agcttcttaa ttaagcaaga attatttacc aaatacgctg aaaatgccat taatttgaat
     1141 caaaaactca gtctgcagtt tagtggcttg tcaactattc cagctaataa cgttttcagc
     1201 gcaaatagtg ctttggcaat gattagtaat aaagctctct tgcaacaatt tgtcagtcaa
     1261 caaagcaaag aagtagtggg tgacattaat ctcctggatc aactagatca agctgctcgt
     1321 caagccaatt tagtcagccc ctactcttct ttaattgcct tggttaatca acagcagatt
     1381 gaaactttga agcgactaat ggagcaatac aatcgtttcc aagataccca aatgattgaa
     1441 aatgaaagcc gaattaacgt tttttgggat ggacccattc cacaacccat gcccatattc
     1501 gacgactttt ccatggaaat gatacctggt tcaccaatga tcaataaagg tgttattggt
     1561 ggagagatga tgaaacaaga agcaatactc aacaatgatt tattatcgct tgataaagat
     1621 gtagctgatg gcgttactgc tcagagaaga aatgctggtg gtttgggagg ttttgctacc
     1681 ggaacaggta tcttatctaa tttatcgggc ggcgctgcat tcataatcgg cactggctta
     1741 attgctgttg tgggcttggt agtttctcta ctgcagcaat tgaaaaaaaa gaaaaaataa
     1801 gattattttt agctaaaatt gtagaccact accttgcctt ggcgtatttc ttaggtgtct
     1861 accttcaaga cttgggtcta aattctggta aaaacggcgc attggttctt ttctcgccca
     1921 gaattcgtta aaacctaagc tatcaatcgc aaaagcagcc aaaataatcg ccaacactaa
     1981 acccacaata atcaacaaaa aattcttctt ataagaaaaa tcatattttt ttaataattt
     2041 aactccaccc cacaatgcta agacagccaa aattggtatc cacagtggta actgactaag
     2101 taacgtctct aaacgccaca agcgcattgg tccctgatgg cgcaacataa agacggttaa
     2161 attaaagaag aacactgcca gcactagcaa aaccaccaca gcaaaaaata acaaactgga
     2221 acccaaaaca aagtaccact tgggtttcat gttaatttcc ccagttttca ccttgctcat
     2281 gatttcttga ctaaggtcaa gctctttagt cgccggtctt tttgctgttt tagacatcga
     2341 ttttttcatc gtttggttca tcgttggttt cttgcttgct attttttttg tttttggcat
     2401 atttttttca tgataatttt ggctcgattg agtctggtcc cgactgtacc agtggctatt
     2461 cgtaaaatat cactaatggc ttcataggat tgctcttcca aataatatag ggttaaaact
     2521 tctttataaa tgattggcat ctgcgccaag caatttttgg ccagattagc tagctctttt
     2581 ttaataaaat catcttctaa atcaaggtcg ctggcaaaat cgacatcttt gagaatgggc
     2641 acttctcgtt ggtgacgatt gagtctattc attgcttcgt tgtgcgtaat gcgatacaac
     2701 caactactaa actttttctt aatattaaat gactgcaaat taacaaaagc cttaataaag
//
LOCUS       DAOSEW010000078         2672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_36785_length_2672_cov_4.443638, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000078 DAOSEW010000000
VERSION     DAOSEW010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36785_length_2672_cov_4.443638"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..769
                     /locus_tag="PLJ02_03125"
     CDS             161..769
                     /locus_tag="PLJ02_03125"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOI05430.1"
                     /translation="MNLQTFILGDLQTNCYLLENGHDAIIIDPADDGDFLNEELSRHN
                     LTLQAVILTHGHFDHCLGLLSLATAWPNLPIMLHSADNFLIDQAQDSAHHWLKRAVDP
                     VPKPNRSLSDGQIINFANETLRVIHTPGHTPGSIALLSNKQGNDETYLFTGDTLFANG
                     VGRTDFAYSNHQHLLKSLTKLKQLKEAGAYSLALPGHGSSFY"
     gene            828..1025
                     /locus_tag="PLJ02_03130"
     CDS             828..1025
                     /locus_tag="PLJ02_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05431.1"
                     /translation="MTKESKTQLRQETQPELTEKATIIEEAKRVARAMEHNPEEMRRA
                     KLREMFPELSDQEIENLLEKA"
     gene            complement(1047..1625)
                     /locus_tag="PLJ02_03135"
     CDS             complement(1047..1625)
                     /locus_tag="PLJ02_03135"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HOI05432.1"
                     /translation="MKKTNRANVKVTLIVATSKDGYISPADGVTLPSTKWTSQEDHQF
                     FTEKTQEISVMIMGRATYDTIGRPLPRRVIVVLTRDSEKQAAAARRKLKMDYLPNNLR
                     FTSKSPQEVLQALATEGYQQVALCGGASLYQLFTAEDLVDEMFITVEPIEFGDGIKLF
                     EDEQIFTNFQLVQETPLNKQGTIVQHWVRKAD"
     gene            complement(1612..2391)
                     /locus_tag="PLJ02_03140"
     CDS             complement(1612..2391)
                     /locus_tag="PLJ02_03140"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:NF014098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HOI05433.1"
                     /translation="MIKIGQQLRPIINQSIKENFPDGLPERSVVVVTSKIIAYEQGRL
                     VPTLAAERGREVTREEERARKHQLVRQEADYYLDPHSSKYDLMLTITEATLMINAGID
                     ESNANEHYVLWPKNLYGEAEKIWRNLREDFGVKEVGVIIPDSRSVPLRWGVVGMSLAS
                     CGFQPLNNRVGDKDLFGREIKMVQVNVAEALATAAVFEMGEVEEQTPLAVITDSQMIK
                     FMDRPLNEQELADLRIEAEDDMYGPLLTSVDWQKGQRNEKN"
     gene            2458..>2672
                     /locus_tag="PLJ02_03145"
     CDS             2458..>2672
                     /locus_tag="PLJ02_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOI05434.1"
                     /translation="MEKQRSQYQQLLAEILNHGQRVSTPQGVDALTIIGPKPMHFKLA
                     EGFPAITERKLSEKIWRQAIAEIIAFI"
BASE COUNT          811 a          606 c          483 g          772 t
ORIGIN      
        1 gcttgccaaa tctagcgcag cagcacctgc tgtcttataa actggtaaag gcaaattttt
       61 atcgaaacgc ttaattttaa ctttcatctt gttactggtt tgctcagtca atgctgcgac
      121 aatgctaaca aatttttgac aaataagcta gactaaagtg atgaatttac aaacttttat
      181 cctcggagat ttacaaacca attgctatct gctagagaac ggccacgacg caataattat
      241 cgatccggcc gacgatggtg attttttaaa tgaggaatta agtcggcaca atttaaccct
      301 acaagcggta attctgactc atggacattt cgaccactgt ttgggcttgt taagcttagc
      361 cacagcctgg cctaacttac caatcatgct gcatagcgct gataattttt taatagacca
      421 agcccaagat agcgcccacc actggttaaa aagagcagtt gatccagtac ccaaacccaa
      481 tcggtcgcta agcgatggcc aaatcattaa ttttgctaat gaaacgctca gagtcatcca
      541 cactcctggg cacacaccag gaagcattgc cctgttgagt aacaaacaag gcaatgatga
      601 gacctattta tttactggag atacactctt tgctaacgga gtgggtcgta ctgattttgc
      661 ttatagcaat catcaacatt tactaaaaag cttaactaaa ctcaaacaat tgaaagaagc
      721 tggtgcttat tctctggctt tacctggtca tggtagcagt ttttattaag caaactccca
      781 acaaacacgc ttggcaaaat ccttattaac cgttaaaatg aaaacatatg accaaagaaa
      841 gcaaaacaca attaagacaa gagacacaac cagagttaac tgaaaaagcc acaattattg
      901 aagaagctaa aagagtggcc agggctatgg aacataatcc tgaagaaatg agacgcgcca
      961 aacttaggga aatgttcccc gaacttagtg atcaggaaat tgaaaatctc ctagaaaaag
     1021 cctaaaaact tgctgattga ttgattctaa tctgcttttc ttacccaatg ttgcacaatt
     1081 gtgccttgtt tattgagtgg ggtttcttgg actaattgaa aattagtaaa tatttgctcg
     1141 tcttcgaata atttaatacc atcaccaaac tcaattggtt ccacagtaat aaacatttcg
     1201 tcaactaaat cctctgcagt aaaaagttgg taaaggctgg ctccaccgca caaagctact
     1261 tgctgatagc cttcagtcgc taaagcttgt aaaacctctt ggggggattt gctggtaaag
     1321 cgcaaattat taggcaaata atccatcttt aattttcgcc gagcggcagc tgcttgtttt
     1381 tcactatcac gcgtcaaaac cacgatcacg cgtcttggca acggccgacc aatggtgtca
     1441 taagtggctc gacccataat catcacgctt atttcttggg ttttttccgt aaaaaattgg
     1501 tggtcttctt gactcgtcca cttggttgaa ggcaatgtca ccccatcagc aggactaata
     1561 taaccatcct tactagtggc cacaatcaaa gtaactttga cattagctcg gttagttttt
     1621 ttcatttctt tgaccttttt gccaatccac actagtcaac aagggaccgt acatatcatc
     1681 ttcggcttca attctgagat cagctaattc ttgctcattg agtggccgat ccataaattt
     1741 aatcatttgg ctatcagtaa tcacggctag tggtgtttgc tcctctacct cacccatttc
     1801 aaaaacggct gcggtagcca aagcttcagc gacattaact tgcaccattt taatttctct
     1861 accaaataaa tctttgtcac ctacccgatt atttaaaggc tgaaacccac agcttgcgag
     1921 tgacatacca actactcccc aacgtagtgg caccgaccga ctatcaggga taatcactcc
     1981 cacttcttta actccaaaat cttctctcaa attacgccaa attttctctg cttcgccata
     2041 aagattcttt ggccatagaa catagtgctc attggcattg gactcatcaa tacccgcatt
     2101 aatcattaaa gtggcttcag taatcgtcag cattaaatca tacttcgaac tatgggggtc
     2161 taaatagtaa tcggcttctt gacgaaccag ctggtgcttg cgagcccgtt cctcttcccg
     2221 agtaacttct ctaccccgct cggctgctaa agttggtact aaccgcccct gttcgtaagc
     2281 aataatcttg gaagtgacca ccaccactga acgttctggt aaaccatctg gaaaattttc
     2341 tttaatactt tggttaataa ttggtcgcag ttgctggcca attttaatca taggagtttt
     2401 gagagcaatc actttcataa aattctttca attcggcttt atgatatatt agaaactatg
     2461 gaaaaacaac gttctcaata tcaacaatta ttggccgaaa ttcttaatca tggacaaagg
     2521 gttagcactc ctcaaggagt agatgcgctg accattatcg gacccaagcc catgcatttc
     2581 aaattagccg agggttttcc agcaattacc gagcgtaaat taagtgaaaa gatttggcga
     2641 caagcgattg cagaaattat tgcttttatt aa
//
LOCUS       DAOSEW010000079         2666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_36915_length_2666_cov_3.788970, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000079 DAOSEW010000000
VERSION     DAOSEW010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36915_length_2666_cov_3.788970"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="PLJ02_03150"
     CDS             complement(<1..352)
                     /locus_tag="PLJ02_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05435.1"
                     /translation="MSVNNKQFFNYLDFLLTLVVSFLLIFFTLRVKTVECYHQDSADQ
                     EELASVCAQLKKNFLGKSLIFYDFFAADVWQELAQSVDYQQLYQLSGLEKKAPSTLIL
                     HLDSKLPDYRLVVFI"
     gene            complement(336..1454)
                     /locus_tag="PLJ02_03155"
     CDS             complement(336..1454)
                     /locus_tag="PLJ02_03155"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HOI05436.1"
                     /translation="MKIFMTGAHLTPALAMIDYIQLAHPNDQLVFLGRLYSQERLKQK
                     AVEELEVKKRGVKFIPFAAVKFVNSSVIDLILAVFTFPGTIGKARKILRQEKPDVLLS
                     FGSYLAVPFVLAAKSLKIPVVTHEQTIAMGKANQFIANLADKVAISFPQTSQYLKKDN
                     FYLTGNPVRQAIFKKNLKKPAYLPSKTNNLILIMGGNQGSFVINNLVKSVLKDLLKEF
                     TVVHQCGRANSLKNYPAELTKIKATLPSKMQNRYFVREWIEEEELFWLYQNSLLAVSR
                     AGANTVLELCLARLPSILIPLPNTFNNEQVLNAKMMVKAGGAIIIEQKKLTAPVLLDA
                     IATVHKHHQEMRQKLAKKNFYQEAEAKIYQLLVDVCQQ"
     gene            complement(1459..2553)
                     /locus_tag="PLJ02_03160"
     CDS             complement(1459..2553)
                     /locus_tag="PLJ02_03160"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOI05437.1"
                     /translation="MSKANWRFTILVLILAVTGLFFLFEASTVESVRLFNHPYYFVTQ
                     QAKWLVLAIILASITSFINLQIFQKLALPLYVVGLISLALPLLPGLGLTLNGASRWIN
                     VFGYSFQPVEIFKFFFINFYASLLAKKTNLASFIFFLIWPSLILLLQPDFGSLLVIIF
                     SAMVMYFLSGVDLKLLALVVTGSFILGLVAILLIPYRRERLMSFVKQDVDIQAEAYHS
                     HQVLLALGAGSWFGRGIGNSQQKYAYVPEASSDSIFAIIAEEVGFVGSSIIILLFAAY
                     FFLAEAMIKEQKLSTYQFLLFYGILAWIAGQLLFNLGAVVAILPLSGMPLPFFSQGGS
                     ALVSVFFVSAILLNISKGQKISKSQSKMLR"
BASE COUNT          944 a          515 c          455 g          752 t
ORIGIN      
        1 taatgaatac taccaaccga taatcaggta atttgctgtc taaatgtaaa atcagggtac
       61 taggcgcttt tttctctaag ccagaaagtt gataaagttg ctgataatca acactctggg
      121 ctaattcttg ccagacatct gctgcaaaaa aatcatagaa gattaatgac ttgcctaaaa
      181 agtttttttt caattgggca caaacactgg ccaattcctc ttgatcggca ctatcttggt
      241 gataacactc gactgttttt actcgtaaag taaaaaaaat cagcaaaaaa ctaacaacta
      301 gggttagcaa aaaatcaaga taattaaaaa attgcttatt gttgacagac atcgactagt
      361 aattgataaa ttttggcttc agcttcttga taaaaatttt tcttagccaa tttttggcgc
      421 atttcttgat ggtgcttgtg cacagtggct attgcatcaa gtaatactgg agcagttaat
      481 tttttttgtt caataataat tgctccccct gctttaacca tcattttggc gttaagcact
      541 tgttcattat tgaaagtgtt tggcagggga atcaaaattg agggtaatct ggctaaacat
      601 aattctaaaa ccgtattagc tccagctctg ctcaccgcta gcaaggagtt ttgataaagc
      661 caaaataatt cttcttcctc aatccattct ctgacaaaat agcggttttg cattttggag
      721 ggtaaagtcg ctttaatctt agtcaattca gcaggataat ttttcaaact attagcgcgc
      781 ccacattgat gaaccactgt aaactctttg agtagatctt ttaaaactga tttgactaag
      841 ttattaatta caaaagaacc ctgattacca cccataatta aaataaggtt attagtttta
      901 ctgggcaagt aagcgggttt ttttaagttt tttttaaaaa tggcttggcg aactggatta
      961 ccagtcaaat aaaaattatc tttttttaaa tattggctgg tctgaggaaa ggaaatggcc
     1021 actttatcgg ctaaattggc aataaattga ttagctttac ccatggcaat cgtctgctcg
     1081 tgagtcacca ctggaatttt taaacttttc gcagccagaa caaagggaac agctaaataa
     1141 gaaccgaaag ataagaggac atcgggtttt tcttgtctta aaattttcct agctttaccg
     1201 atggtaccgg gaaaagtaaa aacggctaaa atcaagtcga tcacagaaga attaacaaat
     1261 ttgactgcgg caaaaggaat aaatttaaca ccgcgttttt tgacttccaa ctcttcaacg
     1321 gctttttgtt ttaagcgctc ttggctatat aagcgaccaa gaaaaaccaa ttgatcattg
     1381 ggatgagcaa gctgaatata atcaatcatc gctaaggctg gggttaaatg cgctccagtc
     1441 atgaaaatct tcataagatc atcttaacat tttgctttgg cttttactaa ttttttgacc
     1501 tttactaata ttgagtaaaa tcgccgacac aaagaaaaca ctgactaggg ccgaaccacc
     1561 ttgagaaaaa aatggcaatg gcatcccact caggggcaga atcgctacca ccgctcctaa
     1621 attaaataaa agctgaccag caatccaagc taaaatgcca taaaacaata aaaattgata
     1681 agtgctcagt ttttgctctt tgatcatcgc ttcagcgagg aaaaaatagg ccgcaaataa
     1741 caaaataatt atcgaagaac cgacaaaacc cacttcttca gcaatgatcg caaaaatcga
     1801 gtcactactc gcctctggaa cataggcata tttttgttga gaattaccaa tccctcgacc
     1861 aaaccatgag ccagctccaa gagctaataa cacctgatga ctatgataag cttccgcttg
     1921 gatatcgaca tcttgcttaa caaaactcat tagccgttca cgacgataag gaattaataa
     1981 aattgccacc aagcccaaaa taaaggatcc cgtaacaact agggccagga gtttaaggtc
     2041 tacaccagac agaaaataca tcaccatggc actaaagata attaccaaca gtgagccgaa
     2101 gtctggttgt aacagtaaaa tgagacttgg ccaaataagg aaaaaaataa aacttgctaa
     2161 attggttttt ttagccaata agctggcata aaaattaata aaaaagaatt taaatatttc
     2221 aactggctga aaactataac caaaaacatt aatccagcga ctagcgccat tgagcgttaa
     2281 tcctaaaccg gggagcaaag gcaaagctaa agaaattagt cctacgacat aaagaggcaa
     2341 agccaatttt tgaaaaatct gcaaattaat aaaagaagta atactggcta aaataattgc
     2401 taaaacgagc cacttagcct gctgagtcac aaaataataa ggatgattaa atagcctcac
     2461 actttctact gttgaagcct caaataaaaa aaataaacca gtcacagcta aaattaaaac
     2521 taggattgta aatcgccaat ttgcttttga catcaaaata atttaaataa aagctaacca
     2581 taaaccaaac agcgttagca taatttgtat cagccaagct cgttgcacaa tttttggctc
     2641 atcccacccc cgttcttgta aggtca
//
LOCUS       DAOSEW010000080         2644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_37364_length_2644_cov_4.257628, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000080 DAOSEW010000000
VERSION     DAOSEW010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37364_length_2644_cov_4.257628"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="PLJ02_03165"
     CDS             complement(<1..660)
                     /locus_tag="PLJ02_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05438.1"
                     /translation="MLTIIHGDNLVKSREKLFQLKQAAQAKNQNLNTLSAKKITPQIL
                     EQALFAKDLFGQEPCLIIEELHSLPQSKKRTDYLEQIIQASQFLDIVLWEKKSLTKNN
                     LKKLTNAQIYYFPMSKALWQLLDQFSPQAKTKKRQLALLQEAIKQDSAEFCLVMISKR
                     VSDLIAITAGADLAMHPFVKNKLNRQKKNFTLKQLLNLHQKLYLLDNKIKHSANLLDL
                     AS"
     gene            complement(665..1324)
                     /locus_tag="PLJ02_03170"
     CDS             complement(665..1324)
                     /locus_tag="PLJ02_03170"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOI05439.1"
                     /translation="MSLNFSYQLKKSSRTTAIRINIDHQGTVLVTAPSLYPNFLIDKF
                     LKQQEAWIIKKQAEAAKLQVKIKHNETYIFGDKYQIVLNIDPKQRPNIISKDQQIIIT
                     QLSAGPYTNRLERFLKNTAIKYLTQKTAILAKKMKIDYQKITIRQQKSRWGSCSSRGN
                     LNFNWRLIHYPPAIINYVIIHELAHRLEPNHSRQFWQLVAKYDPEYKKHQNALKKRLY
                     N"
     gene            1451..1534
                     /locus_tag="PLJ02_03175"
     tRNA            1451..1534
                     /locus_tag="PLJ02_03175"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1484..1486,aa:Leu,seq:tag)
     gene            complement(1768..2460)
                     /locus_tag="PLJ02_03180"
     CDS             complement(1768..2460)
                     /locus_tag="PLJ02_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05440.1"
                     /translation="MRQKTEKKNNVFFQIFSSLAVISFSTALIIIASVAYNYLYQRQP
                     LTTEPLVLSQILEDHQIISADTTGQQVQSVVETEDARPELIANFLERHQSPLTPYDYY
                     GQKLVEIADRHGLDFRLLPAIAMQESNLCRVTNPQAPHNCLGFGIHSQGTLDFDSYEA
                     GFERAARELKQNYINQGLDTVSLIESKYTPSSNGSWADSVNQWMAEIRYDDRDLGRSE
                     KINTHALEFANE"
BASE COUNT          786 a          437 c          505 g          916 t
ORIGIN      
        1 actcgccaaa tctagtaaat tggctgaatg tttaatttta ttatccaata gatagagttt
       61 ttggtgaaga ttcaataatt gttttaaggt aaaatttttc ttttgtctgt ttagtttatt
      121 tttcacaaaa ggatgcatgg ctaaatcagc acccgcggta atggcgatta aatcgcttac
      181 ccgtttacta atcatcacta gacaaaattc agccgaatct tgtttaattg cttcttgtaa
      241 aagcgctaat tgtctttttt tagttttagc ttgtggactg aattgatcta acaattgcca
      301 aagagcttta ctcatcggaa agtaatagat ttgagcatta gtcaattttt ttagattatt
      361 tttagttaaa gatttttttt cccacaaaac gatatctaaa aactgactgg cttgaataat
      421 ttgttcaagg taatcagttc tttttttgct ttgcggcagt gagtgtaact cttcaataat
      481 caaacagggt tcttgaccaa aaagatcttt ggcaaataaa gcctgttcca ggatttgagg
      541 agtaattttt ttggctgaaa gggtgtttag gttttggttc ttagcttgag ctgcttgttt
      601 aagctgaaaa agtttttccc tggatttaac taaattgtcg ccgtgaataa ttgttagcat
      661 ctcattaatt atatagcctt ttctttaaag cattttgatg ttttttgtat tctggatcgt
      721 atttagctac caactgccaa aattgccgcg agtgattggg ctccaagcga tgagctaatt
      781 catggatgat gacataattg ataatggccg gtgggtaatg gatcaagcgc caattaaagt
      841 ttaaattacc tcgactacta caactgcccc aacgtgattt ttgttggcgg atcgtaattt
      901 tttggtagtc aattttcatt tttttagcca aaatagctgt tttttgggtc aaatatttaa
      961 tagcagtatt tttcagaaaa cgttcaagtc tgtttgtgta tggaccagca ctgagttgag
     1021 taatgataat ttgctgatct ttgctaatga tattgggtct ttgcttagga tcaatgttta
     1081 acacaatttg gtatttatca ccaaaaatat atgtttcatt gtgcttaatt tttacctgaa
     1141 gtttagcggc ttcggcttgc tttttaatta tccaagcctc ttgttgtttt agaaacttgt
     1201 cgattaaaaa attaggataa agactaggtg cggtaaccag gacagttcct tgatgatcaa
     1261 tattaattct gattgctgtt gttcgagaac ttttttttag ttgataacta aagtttaaag
     1321 acacagcctt agcttaagaa aaaaaaacta aaagtcaatg agaaattaaa ttgactttct
     1381 ctaatctagt gagatcagtt ataataatta gctgattttt aattagaata aaatagaaat
     1441 ttaaaatcaa gccaaagtgg cggaatggta gacgcgcacg gtttaggacc gtgtgaggca
     1501 actcgtggag gttcaactcc tctcttcggc accagcaact ccgatttttg gtagcacaaa
     1561 cttcaatttt cggcaccacc aactccgatt tttggtagcc aactgagaat ttaccacaaa
     1621 aaaacccgct caaattggcg ggattttttg agctcagaac gtaatcccgg tgaggaagaa
     1681 ccttctgaga tgatgttatt ataacaaaaa ttataaggat gtcaagtcaa gaacgatgaa
     1741 ctagcgattt gcaaaattcc gttattttta ttcgttagca aactctaagg catgagtgtt
     1801 aattttttca ctacgaccca aatctcggtc atcatagcga atttcagcca tccattgatt
     1861 gactgaatct gcccaagaac cattactaga gggtgtgtat ttactctcaa ttaaactgac
     1921 tgtgtctaaa ccttgattga tgtaattttg tttcaattcg cgagcggccc gttcgaaacc
     1981 agcctcatag ctatcgaaat ccaaggttcc ttgactatgt ataccaaaac ctaagcaatt
     2041 atgaggagct tgagggttag taacacgaca aagatttgat tcttgcattg cgattgcggg
     2101 caataaacga aaatccaggc cgtggcgatc ggcaatttca actagttttt gaccataata
     2161 gtcgtaaggg gttagtggtg attggtgccg ctccaaaaaa ttggcaatta attctggtct
     2221 ggcatcttca gtttcaacca cactttgcac ttgttgtcca gttgtgtccg cactaatgat
     2281 ttggtgatct tccaaaattt gacttaaaac caggggctca gtggtcagtg gttggcgctg
     2341 ataaaggtaa ttatacgcaa cactagcgat aataattaaa gcagtactaa aagagatcac
     2401 tgctaaacta gaaaatattt gaaaaaaaac attgtttttt ttctcagttt tttgcctcat
     2461 gctatgttta gattttacta agtctatcat atactagtca attgacttta acaagaattt
     2521 ttgttagaat atgatagata aaatgttgaa atatcaatct aaattgttat ttattctttt
     2581 tttgctgact tgcttccttt gtagttttaa accagcgcaa gcggctggtg aggtaatggg
     2641 catg
//
LOCUS       DAOSEW010000081         2627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_37746_length_2627_cov_3.524106, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000081 DAOSEW010000000
VERSION     DAOSEW010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37746_length_2627_cov_3.524106"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PLJ02_03185"
     CDS             <1..322
                     /locus_tag="PLJ02_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020028581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOI05441.1"
                     /translation="TKVAVKLTLPLKSEKNKKNSLMLKSGKQSSKLFIHPPFKFAMVD
                     ALITNFHLPQSSLLMMVSAFTSQPNSPFVFEKFSQSQIGQAYQHAIQNDYRFFSFGDA
                     MLIV"
     gene            351..1544
                     /locus_tag="PLJ02_03190"
     CDS             351..1544
                     /locus_tag="PLJ02_03190"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOI05442.1"
                     /translation="MPTIKNQTLTLSEDKKLVLKQILHWFEFKTDQQQYLTIGGYAGT
                     GKTTLIAVLRRLLYKKSPKLKVAFAAFTGKATQVLQNYLTEFDLLYRQDSISTIHSLI
                     YSVVKNEQQQIVAWRKKDRLQTDLIIVDEASMIDQEIWQDLLSYQIPIIAVGDHGQLP
                     PIHGYFNLMKEPILRLETIHRQELNNPIIEVSILARTKGYIPVAKYGKKVRKLLKRDP
                     ETAEQVENYLQMADEETLIVCGYNNTRLRLNQYLRTLKGFESEEPQAGDRVICLRNNQ
                     QKQIYNGMLGTLEHIRRIDKNFFEVSVDFDDQNQLYEGLIVAKQFNSPQTISFNGQDR
                     YLLKKADLFDFAYALTVHKAQGSQAKRVIVFEERFKQMSDEDWRRWLYTAVTRAEEEL
                     IIIGD"
     gene            1613..2362
                     /locus_tag="PLJ02_03195"
     CDS             1613..2362
                     /locus_tag="PLJ02_03195"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOI05443.1"
                     /translation="MSVRINKLLADLGISSRRGADLLISAGKVKINGRKAKLGEKVDL
                     NKDKLVVNGELINPAILQKLEGGANYEYWLLNKPPALISAVSDPDQRQTVTHYLKNKT
                     QTRLYPVGRLDYESEGLLLMTNDGELTHRLTHPQYEVEKEYLVDATGIFSQSKIARLE
                     KGVLLAGRKAKADRVELLKKDGRNLQLKFILHEGRKREVRRLCARVGWEVTRLVRVRL
                     GNIELANLEPGTVRKLTEAEIRELKASVKLV"
     gene            2391..>2627
                     /locus_tag="PLJ02_03200"
     CDS             2391..>2627
                     /locus_tag="PLJ02_03200"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOI05444.1"
                     /translation="MTNDFKYLIFDFDGTLADTAGIQVRAFNQIHARFDLPKIGKKEL
                     MKLHSTSMKEICKEYQIGPIKLLKITEALNQEVSA"
BASE COUNT          883 a          436 c          531 g          777 t
ORIGIN      
        1 taccaaggtc gcggtcaagc ttaccctgcc ccttaaatcc gaaaaaaata aaaaaaattc
       61 tctaatgtta aaaagtggca aacaaagtag caagctgttt attcatccac cttttaaatt
      121 cgccatggtt gatgctttaa ttaccaattt tcatttgcca caatcaagtt tattaatgat
      181 ggtatcggcc tttactagcc agcccaattc cccctttgtc tttgaaaaat ttagccagtc
      241 tcaaattggt caagcttacc aacatgctat ccaaaatgat tatcggtttt ttagttttgg
      301 tgatgcgatg ttgattgttt aggcttttct tgctaggctg atcggagatc atgccaacca
      361 tcaaaaatca aaccttaacc ttgtcagagg ataaaaaact tgttttaaag cagattttac
      421 actggtttga atttaaaact gaccaacagc aatatctaac gatcggcggt tatgcgggca
      481 ctggcaaaac tactctaatt gcggtactgc gtcgcctact ttataaaaaa tctcccaaat
      541 taaaagtagc ttttgctgcc tttactggta aagcaacaca ggttttacag aattatttaa
      601 cagaattcga tttgctgtat cggcaagatt cgatcagcac catccattca ttgatttata
      661 gcgtagtgaa aaatgagcaa caacaaattg ttgcttggcg taagaaagat cgtttacaaa
      721 ctgatttaat tattgttgat gaggcatcaa tgatcgatca ggaaatttgg caggatttgt
      781 tatcttacca aatacccatt atcgctgtgg gcgatcatgg tcaattacca ccaatccatg
      841 gttattttaa tttaatgaaa gagccgattc tacgattaga aacgatacac cgtcaagaac
      901 tcaataatcc cattattgag gtatcaattt tagcgagaac taaaggttat attccagtgg
      961 ctaaatatgg aaaaaaagtg aggaaattat tgaagagaga tcccgaaact gccgaacaag
     1021 tggaaaatta tttacaaatg gcagacgaag aaacactaat tgtttgtggc tacaacaaca
     1081 ctcgattgcg acttaatcaa taccttagaa cgctaaaagg ttttgaatcg gaagaacctc
     1141 aagctggaga tcgggtgatc tgtttacgta acaatcaaca aaagcagatt tataacggta
     1201 tgttagggac attagagcac attcggcgaa ttgataaaaa tttttttgaa gttagtgttg
     1261 attttgatga tcaaaatcaa ctttatgaag gtttaattgt ggctaaacaa tttaacagtc
     1321 ctcaaacaat tagttttaat ggtcaagata gatatttact caaaaaagct gatttgtttg
     1381 attttgctta tgcgctgacg gtccataaag ctcaaggtag tcaagccaaa cgagtaattg
     1441 tttttgaaga gcgctttaaa caaatgtctg atgaggattg gcgacgctgg ctttatacag
     1501 cggtgacgcg agccgaggaa gaactaatta ttattggtga ttaactggct aaattaaatt
     1561 tgaaagatct cgattttatt aatcatttag tgtatataat taacggagat ttatgagtgt
     1621 gcgcatcaac aaactactag ctgatttagg aattagttct cgccgaggag ctgacttatt
     1681 aattagtgca ggcaaggtta aaatcaacgg tcgcaaagca aaattaggag aaaaagttga
     1741 tttaaacaaa gataagttag tggtcaatgg ggaactgatc aatccagcaa ttttgcaaaa
     1801 acttgagggc ggagcaaatt atgaatattg gctattaaat aaacccccag cgttaatttc
     1861 agcggtcagt gatccagacc agcgccaaac agttactcat tatttaaaaa acaaaacaca
     1921 gactcgttta tacccggtgg gtcgtttaga ttatgaatct gaaggcttat tgttgatgac
     1981 taatgatggt gagttgactc atcgtttaac tcatcctcaa tacgaagtgg aaaaagagta
     2041 tctagttgat gccactggaa ttttttctca gtcaaaaatt gctagactgg aaaaaggagt
     2101 attgttagct ggtcgaaaag ctaaggcaga tcgagttgaa ttattaaaaa aagatgggcg
     2161 taatttacaa ttgaagttta tccttcacga gggtcgaaaa cgtgaagtgc gccgactctg
     2221 tgctcgagtt ggttgggaag taactcgtct agttagagtg cgtctgggca acatcgagtt
     2281 ggctaattta gaacctggga cggttcggaa attaacagaa gcagaaattc gagaattaaa
     2341 agctagtgtc aagttagttt aactagcagt tgctaaatga aaaggaagta atgactaacg
     2401 actttaagta tctaattttt gattttgatg gcactctggc tgatacggct ggtattcaag
     2461 tgagagcttt taatcaaatt catgcccgat ttgatttacc aaaaattggc aaaaaagaat
     2521 taatgaaact tcactcaact agcatgaagg aaatttgcaa agaatatcag attggtccaa
     2581 ttaaattatt aaaaattacg gaggcgctca atcaagaagt aagcgct
//
LOCUS       DAOSEW010000082         2552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_39405_length_2552_cov_3.509011, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000082 DAOSEW010000000
VERSION     DAOSEW010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39405_length_2552_cov_3.509011"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PLJ02_03205"
     CDS             <1..291
                     /locus_tag="PLJ02_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05445.1"
                     /translation="KDSVGHRISLDAVAQETLGSGKIGDGLDAIRYYHNGNLEALKKY
                     CLEDVKITRDIYDYGLKNGLVLFRNKWNRLIKAPVNFTFVAKKASGTQMSLI"
     gene            432..932
                     /locus_tag="PLJ02_03210"
     CDS             432..932
                     /locus_tag="PLJ02_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOI05446.1"
                     /translation="MKQQLTDRVKFLHKVVIINSASGQPQALILKRASDASSRPNAWD
                     LPGGNAEWPAKMQESAANLHQLDIAREVKEESHLTVDPKLFNLDHLVYFSSYFEADRQ
                     IYTIICGWAVEFRETDQADISISDEHQAQAWISRDELDNYDFAGEKGQFIKEMISSAF
                     IKYNHQ"
     gene            979..1698
                     /locus_tag="PLJ02_03215"
     CDS             979..1698
                     /locus_tag="PLJ02_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOI05447.1"
                     /translation="MMQKILEINKLNVAVNDQAILKDLSLTINEGELVALMGANGAGK
                     STLVRALLGDETYQVVSGQITFRKKDLLSLSPEERAKLGLAFVWQQPPAIKGVELFTL
                     LNLIQEIHDGDYDYQLADSLLFRDINVNFSGGEKKIAELVQVLNQHPRLLVIDELDSG
                     LDMENLQIVLTALKKELKKSQMALLIITHSGAVLEEIKPDRIAILRQGELICQSRDIK
                     KVLSTIKKYGYQKCESCQNCL"
     gene            1658..>2552
                     /locus_tag="PLJ02_03220"
     CDS             1658..>2552
                     /locus_tag="PLJ02_03220"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOI05448.1"
                     /translation="MATKNVKAVKTAYKQLDHHIEEILHMSGSEVMSSVDAWNNLPWT
                     RQYFGDQPKEGYLIWIKEQPDCSFLACLSLDKPGTKQQLNNLIVIETGLYVDLTGTCE
                     SASTLTTTTAGDFRSHQAKGKLILKKGSSLYYQHLHDWDKNDRVSTNYEFFLEEGASL
                     AYTYKIEAAAAVMNMQAQMHLAKGARSDLKILADCQETKIDLTDTAYLEGAGANAIST
                     LRIVGRKNATVKARSKIIAQAAAKGHLDCGALMLDKTAKLTLIPELLVANQDAQVTHE
                     ASIGKLSAEQILYLRSRGLTAK"
BASE COUNT          829 a          463 c          543 g          717 t
ORIGIN      
        1 aaagatagcg ttggccatcg gattagttta gatgcagtag ctcaggaaac ccttggatcg
       61 ggtaaaattg gtgatggttt ggacgcgatc cgttattatc ataacggcaa tctagaggcg
      121 ttgaaaaaat attgtttaga agatgtcaaa attactagag atatttatga ttacggttta
      181 aaaaatggtt tagtactgtt tcgcaacaaa tggaatcgtt tgattaaagc tccagtcaat
      241 tttacttttg tagccaaaaa agccagtggc acccaaatgt ctttaattta agtttacgtc
      301 agcacttaaa ccgcgatttt gttacactga attgctcggg tgaattatca gaaccgtctg
      361 gctggtaaaa aaatcagaag agttagcttg gtttacattt taactgatat aatcgctaag
      421 tggagattct tatgaaacag caactaaccg atcgagtcaa atttttacat aaagtggtca
      481 ttatcaacag cgccagtggg caacctcagg ctctgatttt aaaaagggcc agtgacgcta
      541 gttcccgacc taatgcctgg gatctgccag gtggcaatgc cgagtggcca gcaaaaatgc
      601 aagagagcgc tgctaacttg catcaattag acattgctcg tgaagtcaaa gaagaaagcc
      661 atttaacagt tgatcccaaa ctttttaatt tagatcattt ggtttatttt tctagctatt
      721 tcgaagctga tcgacaaatt tatacaatta tctgtggctg ggcggtggag tttcgcgaaa
      781 cagaccaagc cgacattagc atttccgacg agcaccaagc acaagcgtgg atcagtcgtg
      841 acgagttgga taattatgat tttgctggag aaaaaggtca gtttattaaa gagatgatta
      901 gcagtgcatt cattaagtac aaccatcaat aaatacaaca aatcaacaac tacaacaaat
      961 cactaagtac aacaatcaat gatgcaaaaa attttagaga tcaacaagct caatgtggca
     1021 gtcaatgatc aagctattct taaagacttg tcgttaacta ttaatgaagg ggaattagta
     1081 gcgctcatgg gcgccaatgg ggctggtaag tccactttag tgagagcttt attgggtgat
     1141 gaaacttatc aggtagtgag tggccaaatt actttccgca aaaaagattt gttgagttta
     1201 agtccggaag agcgagctaa gctaggtctt gcttttgttt ggcaacagcc accagcgatc
     1261 aaaggagttg aattgttcac tttactgaat ttaattcaag agattcatga tggagattat
     1321 gattaccagt tagcagactc tcttttattc agggacatca atgttaattt ctctggagga
     1381 gaaaaaaaga ttgctgaatt agttcaagtt ttaaatcaac atcctcggtt gttagtgatt
     1441 gatgagttgg attctggctt ggacatggaa aatttgcaaa tagtcttaac tgccttaaaa
     1501 aaagaattaa aaaaaagtca aatggctctg ctcatcatca ctcactcggg agcagtttta
     1561 gaagaaatca aaccagatcg cattgccatt ttgcgtcaag gagaattaat ttgtcagagt
     1621 cgagatatta aaaaagtgct tagcactatc aaaaaatatg gctaccaaaa atgtgaaagc
     1681 tgtcaaaact gcttataaac aactagatca tcatatcgaa gaaatactcc acatgtctgg
     1741 ttctgaagtg atgagttctg tggatgcttg gaataatctg ccttggacta gacagtactt
     1801 tggtgaccag ccaaaggaag gttatctcat ttggatcaaa gaacagccag attgttcttt
     1861 tttagcttgt ttgtccctag ataaacctgg aactaaacaa caacttaata atttaatagt
     1921 tattgaaacc ggtctttacg ttgatttaac agggacttgt gagtcagcaa gtacattgac
     1981 tacaactaca gcaggtgact ttcgttctca tcaggcaaag ggaaaattaa ttctcaaaaa
     2041 aggatctagt ctttattatc aacatttaca cgactgggat aaaaatgatc gagttagcac
     2101 taattatgaa ttctttttag aggaaggtgc tagtctcgct tacacttata aaattgaggc
     2161 cgccgccgcc gtcatgaaca tgcaagcgca aatgcactta gccaaaggcg cccgctccga
     2221 cttaaagatt ctggcggact gtcaggaaac gaaaattgac ttaacagaca ccgcctattt
     2281 agaaggggcg ggcgccaacg ctatctccac tctccgcatt gttggtagaa aaaatgcaac
     2341 agtgaaggca cgctctaaaa tcattgctca agctgcggct aaaggtcatt tagattgtgg
     2401 agccttaatg ttggacaaaa cagcaaaatt aaccttgatt cccgaattat tagtagcaaa
     2461 tcaggatgcc caagtgactc atgaggcgtc aatcggtaaa ttgtcagcag aacaaattct
     2521 ttacctcaga agccggggtc taacggctaa gg
//
LOCUS       DAOSEW010000083         2511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_40372_length_2511_cov_3.801710, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000083 DAOSEW010000000
VERSION     DAOSEW010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40372_length_2511_cov_3.801710"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PLJ02_03225"
     CDS             <1..794
                     /locus_tag="PLJ02_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05449.1"
                     /translation="QEPPTINVNVPEQEPPTINVNVPEQEPELVAPVEPLVLPNCPLE
                     DQGKQIKFAETLEKKDGIYYLSSQIEHLQPYLGCNVLVEGRAELINEHHIWILLGQWQ
                     LSIREGSLWVYPADWNMADFSTEKPPVAAEFVTAKRQNQVANGYDWIIFVHDGENNYV
                     FPAGQDTPLVVLPDNAGFSEPQWISVHGIWDQKSTAFNASIGAEGATTVALLDEELFF
                     WDGAQDNILFQSVQAWLMPSTWDQSQIENWAKAQFPEMELQPYAP"
     gene            896..2281
                     /locus_tag="PLJ02_03230"
     CDS             896..2281
                     /locus_tag="PLJ02_03230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOI05450.1"
                     /translation="MKLTSKTYQRFNDPATLLVVSSFPLQGEEIAKRNAVSRYSYLLL
                     KHFPDQQRVVVLCEQVVGQNNQPYLLKKNILVIPSYPINSLKLFTQLNLQLKRFSQVK
                     NILLQFEFSLFGKEVITFLLPFFFIWQRVLGKKIYTMLHQVVLDLSTLVGQVALPANS
                     IKTLFFNGAMRVFYSILGLASQKILVHDQFLAEKLCHLVPQRKLAIIPHGINGYQGFR
                     QGQKDKFKSSLGCQSSDQLVLAYGYHSWYKGTDWLINNFLRLKKAGQLTKNTKLLLAG
                     DAAPTQKSQPHLQGFYHKLSKLIKNNSQDVIHTGFVPEKEVAKIFAIADLVVFPYRAR
                     MSSSGALALAWQYRKPFISSKFFAENLTTSELDKLAKKMHLDINSLQFNLNYASFAKT
                     FINSWQNPLLSQKMARLGRQVAKAHDWQEIAGQYLDIIKLAQKQSVTSVKTARVYFWQ
                     KLAYSRVNTPA"
     gene            2302..>2511
                     /locus_tag="PLJ02_03235"
     CDS             2302..>2511
                     /locus_tag="PLJ02_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05451.1"
                     /translation="MKFISLLKKFWPQIFLFFLVTLLFYFNYSPNTYLTGWDNLQVEL
                     DLSINLERALNVSWQEYQGLGLLGGM"
BASE COUNT          720 a          493 c          509 g          789 t
ORIGIN      
        1 aacaggaacc acccacaatc aatgtgaatg ttccagaaca ggaaccaccc acaatcaatg
       61 tgaatgttcc agaacaggaa ccagaattgg ttgcaccagt ggaacctttg gttctaccga
      121 attgccccct ggaagatcag ggcaaacaga tcaaattcgc cgaaactctt gagaagaaag
      181 atggaattta ttatctttca tctcaaattg agcatttaca gccgtatctt ggctgtaatg
      241 tcctcgttga gggtcgtgcg gagctcataa atgagcatca catctggatt ttgcttggcc
      301 agtggcagct gtcgatccgc gagggtagcc tttgggttta ccccgctgat tggaatatgg
      361 ccgatttctc gactgagaaa ccgcccgttg ctgcggaatt tgtgaccgca aagcgacaaa
      421 atcaagtcgc taatggttac gactggataa tatttgttca tgacggggaa aacaattatg
      481 tatttcccgc tggacaggat acacccctcg ttgttttacc cgacaatgcc ggattctcag
      541 aaccccaatg gatttcggtc catgggatct gggatcaaaa gtcgacagcc ttcaacgcct
      601 caatcggcgc tgagggggcc acaactgtag ccttgttgga tgaagaattg tttttctggg
      661 atggtgccca ggacaacatt ctttttcagt ctgtacaggc ttggcttatg ccgagcacct
      721 gggaccagtc ccagatcgaa aactgggcga aggctcagtt tccagaaatg gaactgcaac
      781 cttacgcccc gtaacatttt tcctcctttc ccacgtcttc cttgaaagat aggagacgtg
      841 ggtaaagcaa agctcatcaa tgatcattgg tgatttttgc tttactaata acatcatgaa
      901 attaactagc aaaacctacc agcgctttaa tgatccagct actttactgg tagtttctag
      961 ttttccactg caaggtgagg aaattgccaa gcgcaatgca gtttcacgct atagttatct
     1021 attacttaaa cattttccag accagcaaag agttgtagtt ttgtgtgaac aggttgttgg
     1081 tcaaaataat cagccatatt tactcaagaa aaacatttta gttattccca gttatccaat
     1141 taactcatta aaacttttta ctcagttaaa cctgcaattg aagcgttttt ctcaggtaaa
     1201 aaacattctt ttacaatttg agtttagttt atttggtaaa gaagtgatta cctttttact
     1261 gccatttttc tttatttggc aacgcgtact tggtaaaaag atttatacca tgctccatca
     1321 ggtagtgtta gatttatcaa ctttagttgg tcaggtggct ttacctgcta attcaattaa
     1381 aaccttattt tttaatggag cgatgcgtgt tttttacagc atattgggtt tagctagcca
     1441 aaaaattcta gtccatgatc aatttttagc tgaaaagtta tgccacttag tgcctcaacg
     1501 caaactggct atcattcctc atggaatcaa tggctatcaa ggctttaggc aaggacaaaa
     1561 ggataaattc aagagcagtc tcggctgtca atccagtgac caattagttt tagcttatgg
     1621 ttatcactct tggtacaagg gtactgattg gttaattaac aattttttgc gtttaaaaaa
     1681 ggctggtcag ttgaccaaaa atactaaatt attgttggct ggagatgcag cgccgactca
     1741 aaaaagtcag cctcatttgc agggttttta ccataagctc agtaaattga ttaaaaataa
     1801 cagtcaagac gttattcaca ccggttttgt tccagaaaaa gaagtagcga aaatttttgc
     1861 tattgctgat ttggtggtgt ttccttatcg tgcccggatg tctagttcag gagctttagc
     1921 tcttgcttgg cagtatcgta aaccctttat tagttccaaa ttctttgcgg aaaatttaac
     1981 tactagtgaa ttagacaaat tagctaaaaa aatgcacctt gatattaatt ctttgcaatt
     2041 taatttgaac tatgcctctt ttgccaagac tttcatcaat agctggcaaa accccttatt
     2101 aagtcaaaaa atggctcggc tgggtcgtca agtagctaaa gctcatgatt ggcaggagat
     2161 tgctggtcaa tatttagaca ttatcaagct agctcaaaaa caaagcgtaa cttccgttaa
     2221 aacagctagg gtttattttt ggcaaaaact ggcctattct cgggttaaca ctccagctta
     2281 atatttgtat acaattgagt tatgaaattt atttccttat taaaaaaatt ctggccacag
     2341 atttttttat tttttctagt gacgttgtta ttttatttta attacagccc taatacttat
     2401 ttgactggtt gggataatct tcaagttgaa ctcgatcttt caattaactt ggaaagggcc
     2461 ttgaatgttt cgtggcaaga gtaccagggc ttgggcttgt tagggggtat g
//
LOCUS       DAOSEW010000084         2481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_41094_length_2481_cov_3.995466, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000084 DAOSEW010000000
VERSION     DAOSEW010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41094_length_2481_cov_3.995466"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PLJ02_03240"
     CDS             <1..372
                     /locus_tag="PLJ02_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05452.1"
                     /translation="AVTDIGTGADFNENLYIGLVVTESGEGVNTEAETDIEGVGELAD
                     CLMMRVSYLDMANHEVEVQPYTSFTQLQTMFIDEQNFSPLDDLLQTTTVNLKLDFNAD
                     ACGNEAMGDTVAFDLNFYYEQ"
     gene            437..1447
                     /locus_tag="PLJ02_03245"
     CDS             437..1447
                     /locus_tag="PLJ02_03245"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOI05453.1"
                     /translation="MPRSKKKRSSRSYQRVPKKKTFKDFLSNLFLPSKKNRYRPQLLH
                     PEALMVLSFVVLAGFALFNAIRFFPSLADKILGFATNIDVNQVLTLTNEERAKLGLPP
                     LQINEKLNQAAVAKAQHMFSQQYWAHVAPDGTQPWSFIKNAGYVYKYAGENLARDFDS
                     TGEMMKAWMNSPTHKENIINPNFTQIGLAVVNGTLNGFNTTLVVQMFGTPGAVELKQS
                     ASGEAITLPAPADSRVAQLPDEAMNINAIESELADTNELGLVEGERVASGRMLFSPLY
                     LTKTFFLLVVALIILTLIYDAFVSKNRRYERIVGQNFAHIMLFLTVGFLLILFRGGTI
                     VP"
     gene            1451..1708
                     /locus_tag="PLJ02_03250"
     CDS             1451..1708
                     /locus_tag="PLJ02_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05454.1"
                     /translation="MPKLPKKHRLAYLILFLALAVFAYFFSRSWPNKMLQRYLAVGIG
                     IFYFFWGLITHVRSSKITSDIIFEYLAISMLAVLLLILITL"
     gene            1755..>2481
                     /locus_tag="PLJ02_03255"
     CDS             1755..>2481
                     /locus_tag="PLJ02_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOI05455.1"
                     /translation="MSKKNMKGIILAGGNGTRLSPLTKVTSKQLLPIYDRPMIHYPLE
                     TLRRAGIVDILFIVAPDHAGDFLKYLGSGKEFNCHFSYEIQDKPQGIAQAFQIGEDFL
                     DNDNVTLILGDNLFEDDFTQAIQSFEKGGRIFAKQVADPERFGVVEFDEHNRAISIEE
                     KPQHPKSNYCVTGLYIYDNTVVEKVKNIEFSDRGELEITDLNNLYLKEKTLDVAFVNG
                     AWFDTGTFESMYAATEFARARRLA"
BASE COUNT          724 a          399 c          523 g          835 t
ORIGIN      
        1 gctgttacag acattggcac tggagctgat tttaatgaga atctttatat aggtttggtt
       61 gttaccgaga gcggtgaagg tgtgaatact gaggctgaaa ctgatattga gggagttgga
      121 gaattggccg attgtttaat gatgcgggtt agttatctag atatggccaa tcatgaggtt
      181 gaagtgcaac cttatactag ttttactcag ttacaaacaa tgtttatcga tgaacaaaac
      241 ttttcaccgc tagatgatct gttacaaacg accactgtta atttaaaact tgatttcaat
      301 gctgatgctt gcggtaatga agcgatgggt gatactgtgg cctttgattt gaatttttat
      361 tacgaacaat agctagagtg gcgggtcgag tagtgcttgc ttgactcgcc aatctttgtc
      421 ataatgttta gactatatgc ctaggtctaa aaagaagcga tcttctaggt cgtatcaacg
      481 cgtaccaaag aaaaaaacat ttaaagattt cttgagtaat ctgtttttac ctagcaaaaa
      541 aaatcgttat cgtcctcaac tgttgcatcc tgaggcacta atggttttat cctttgttgt
      601 tttagctggc tttgctctct ttaatgcaat tcgttttttt ccatctctag ctgataaaat
      661 tttgggtttt gctactaata ttgatgttaa tcaggtatta actttaacca atgaagaacg
      721 tgccaaacta ggtttgccac ccttacaaat taatgagaag ttgaatcaag cggcggtagc
      781 taaagctcaa cacatgttta gtcaacaata ttgggctcat gtcgctcctg acgggacaca
      841 accatggtca tttattaaaa atgctggtta tgtttataaa tatgctggag aaaatttagc
      901 tagagatttt gacagtactg gagaaatgat gaaggcgtgg atgaattctc cgactcataa
      961 agaaaatatt atcaatccga attttaccca gatcgggtta gcggtggtta atgggacgtt
     1021 aaatggtttt aatacgactc tagtggtgca gatgtttggt acgccaggag cagttgaact
     1081 caaacaaagt gctagtggtg aagccattac tttgcctgca ccagctgata gccgagtggc
     1141 acaattacct gatgaggcaa tgaatattaa cgccatagag tccgaattgg ccgatactaa
     1201 cgaattaggc ttggttgaag gagaaagagt cgctagtggg cgaatgttgt tttcgccact
     1261 ttatttaact aaaaccttct ttttattagt tgttgcctta atcatcctga ctctaattta
     1321 tgatgccttt gtgtcgaaga atcgtcgtta cgagcgaatt gttggtcaaa attttgctca
     1381 cattatgctg tttttaaccg tgggcttttt attaatttta tttcgtggag gaactattgt
     1441 tccttgattc atgccaaaac tacctaaaaa acaccgccta gcctacctga ttcttttttt
     1501 agctttggcc gtttttgctt attttttttc tcgttcttgg cccaataaaa tgcttcaacg
     1561 ctatttggcg gtaggtattg gcatttttta ttttttttgg ggtttaatta ctcatgtgcg
     1621 cagtagtaaa attaccagtg atattatctt tgagtattta gcgatatcga tgttggcagt
     1681 gttattactg atcttgatta ctctttaaaa attttgtgtt aagatgcgac agtttataaa
     1741 aattgggagt ttgaatgtct aaaaaaaaca tgaaaggtat tattttggct ggaggaaatg
     1801 ggactcgttt gtcaccttta accaaagtta ccagtaagca acttttacca atttatgatc
     1861 gaccaatgat tcattatcca ctagaaacat tacgtcgagc tggcattgtt gatattttat
     1921 ttattgttgc tccggatcat gctggtgact tccttaagta tttgggcagt ggcaaagagt
     1981 ttaattgtca tttttcttac gaaattcaag acaaaccaca aggtattgct caagcttttc
     2041 aaattgggga agattttctc gataacgata atgtcacgct gattttgggt gataatttat
     2101 ttgaggatga ttttactcaa gcaattcaaa gttttgaaaa aggtggcaga atttttgcta
     2161 aacaggtagc tgacccagaa agatttggtg tagtcgagtt tgatgaacat aatcgagcaa
     2221 tttctattga agaaaaacct cagcacccaa agtcaaacta ttgtgtcact ggactttata
     2281 tctacgacaa caccgtagtg gaaaaagtca aaaatataga attttccgat cggggagaat
     2341 tagaaataac tgatttaaat aatttatatt taaaagaaaa gacattagat gtggcttttg
     2401 ttaacggggc ctggtttgat accggtactt ttgaatcaat gtatgcagca actgaatttg
     2461 ctagagcgag gcgattagca a
//
LOCUS       DAOSEW010000085         2471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_41352_length_2471_cov_3.866308, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000085 DAOSEW010000000
VERSION     DAOSEW010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41352_length_2471_cov_3.866308"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="PLJ02_03260"
     CDS             <1..959
                     /locus_tag="PLJ02_03260"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOI05456.1"
                     /translation="AFEQGKKPSDLIDDTSITYQIAGSRPYTPRNYDNRFRGKVTLRE
                     ALASSYNVPAVKLLAELGVQNLIKKGQELGITTWNDPSRFGLSLTLGAGEVLMLDMAE
                     LYSTFANLGYPVESNALLEVKDAYGKLLYVNDCALNRGNCYSSLKLSPQVAYIITDIL
                     SDNQARAGAFGLYSVLNIPGQEVAVKTGTTNSMKDNWTIGYTTDRLVVTWVGNNDNTP
                     MSFVASGVTGASPIWNKIFLLLLDEANPHQFVMPSNLLKVKICATTGTLPCSACPKVV
                     EEIFVVGTEPKNACSDQYFADLAKKQEVQALQEANRPAGQIF"
     gene            complement(962..1162)
                     /locus_tag="PLJ02_03265"
     CDS             complement(962..1162)
                     /locus_tag="PLJ02_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05457.1"
                     /translation="MPQPITNKQLAFDLKKDTQRIEKKYQTNKSRRHKEKKEQEDLSQ
                     QWLAPLLLFLTLLIGYLLYTFY"
     gene            complement(1170..1880)
                     /gene="cmk"
                     /locus_tag="PLJ02_03270"
     CDS             complement(1170..1880)
                     /gene="cmk"
                     /locus_tag="PLJ02_03270"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOI05458.1"
                     /translation="MSKSSDFFQIAIDGPVAAGKGTVSRLLAEKLNFLYVDTGAMYRA
                     AALLAIQNNINFDQEDKVVTLIAQSTLEMRNPEGDKEADGRLTTLILNGKDVSWAIRT
                     TKCSDGASKVATLAKVRQILVEKQKKIAETQNVVMEGRDITFKVLPDADLKIFLTADL
                     EIRAKRRHLQYLTKGIDKSIEEVIKEVQERDQRDMTRKIDPLHPTADSWILDSSHLSI
                     EEVVNLIAEKVNTMIKKK"
     gene            complement(1912..2145)
                     /locus_tag="PLJ02_03275"
     CDS             complement(1912..2145)
                     /locus_tag="PLJ02_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05459.1"
                     /translation="MPKRVFWPATLVIMGLIFMAANIGLLPLALWNLWPIILVLVGLG
                     GLLTSDREEWLVEPKSKKSQSKKSKKKKTAHKK"
     gene            complement(2217..>2471)
                     /locus_tag="PLJ02_03280"
     CDS             complement(2217..>2471)
                     /locus_tag="PLJ02_03280"
                     /inference="COORDINATES: protein motif:HMM:NF013804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HOI05460.1"
                     /translation="NQDYDPKKTRKLLLKRREIEELVTASEQKSYSIIPLAFLLEHNQ
                     IKLELALAKGKKSFEKRETIKKRDLNRELNQKLKRSQFKI"
BASE COUNT          690 a          437 c          480 g          864 t
ORIGIN      
        1 tagcttttga acaagggaaa aaacccagtg atttgattga tgataccagt attacttatc
       61 aaattgctgg tagtcgtccc tatactccac gcaattatga caatcgtttt cgtggcaaag
      121 taaccttgag agaagcttta gcttcttcat acaatgtacc agctgtcaaa ttattagctg
      181 agctaggtgt acaaaatttg attaaaaaag gtcaagaatt agggattact acttggaacg
      241 atcctagccg gtttggtttg tccctgactc taggagcagg tgaagtgttg atgttagaca
      301 tggctgaact ctatagtact ttcgctaatt tgggttatcc agtcgaaagt aatgcattgt
      361 tagaagttaa ggacgcttat ggtaagctgt tgtatgttaa tgattgtgct ctgaatcgag
      421 gtaactgtta tagttctctc aaattaagtc ctcaagtcgc ttatattatt actgatattt
      481 tgtcggacaa tcaggctaga gccggtgctt ttggtttata ttcagtttta aatatacctg
      541 gtcaagaagt ggcggttaaa actggaacaa ctaacagtat gaaagacaac tggactattg
      601 gttatacaac agatcgatta gttgtgactt gggtaggtaa taatgacaat acaccaatga
      661 gttttgttgc gtctggtgtt actggagcat caccgatttg gaataaaatt tttcttttac
      721 ttttagatga ggccaatcct catcaatttg tgatgccaag taatttatta aaagttaaaa
      781 tttgtgcaac aactggtacc ttaccttgta gcgcttgtcc gaaagtggtg gaagagattt
      841 tcgtagtggg tactgaacca aaaaatgctt gtagcgacca gtacttcgct gatttagcca
      901 agaaacaaga ggtgcaagct ttacaagaag ctaatcgacc agctggtcaa attttttaag
      961 tttagtaaaa cgtgtaaagc agatagccaa tgagtaaagt taagaataat aacagcggag
     1021 ctagccattg ttgtgagaga tcttcttgtt cttttttttc tttgtggcga cgacttttgt
     1081 tagtctgata ttttttttca attctttggg tgtctttttt gagatcaaaa gctagttgtt
     1141 tgttggtaat aggttgcggc atcttaggct cacttttttt taatcatggt gtttactttt
     1201 tcagcaatta aattaaccac ttcttcgatg cttaagtgac tcgaatctaa aatccaagag
     1261 tcagcggtgg ggtgcagggg atcaattttc cgagtcatat ctctttggtc tcgctcttgc
     1321 acttctttta tgacttcttc aatactttta tcaataccct tggttaaata ttgtaaatga
     1381 cgacgtttag ctctaatttc taagtcagcg gttaaaaata ttttcaaatc tgcgtctggg
     1441 agaactttga aagtaatgtc gcgcccttcc ataactacgt tttgtgtttc ggcaatcttt
     1501 ttttgctttt ctactagaat ttgtctaact ttggctaaag tggccacttt tgaagctccg
     1561 tcactgcatt tggtggtacg gattgcccag gacacatctt tgccgttcaa aatcagcgtg
     1621 gtcagacgac cgtcagcttc tttatctccc tctggattac gcatttctaa tgtgctttga
     1681 gcaatgagtg tgacaacttt atcttcttga tcaaaattaa tgttattctg aatggctaaa
     1741 agcgcagcag ctcgatacat tgctccagtg tcgacatata aaaaatttaa tttttctgcg
     1801 agtaatctag atacagtacc tttacctgcg gcaactggac catcaatagc aatttgaaaa
     1861 aaatcagaag acttactcat aaagagcttt cgctatggcc tttactaact gttatttttt
     1921 atgggcagtt ttctttttct tacttttctt actttggctt tttttgctct tgggttccac
     1981 taaccattct tctcgatcgg aagtaagcaa acctcctaaa ccgaccaaga ccaaaatgat
     2041 tggccaaaga ttccagaggg caagtggtaa gagaccaata ttagcggcca taaaaattaa
     2101 acccattatt actaaggtag ctggccagaa aactctttta ggcataaatt cctttataca
     2161 taataattgc tacctactat taaaaagcaa attcaatcga caagcaaggg taagttttat
     2221 attttaaatt ggctacgctt taatttttga ttaagttccc ggtttaagtc tcgttttttg
     2281 atagtttctc ttttctcgaa actctttttc cctttcgcta gagctaattc taatttaatc
     2341 tgattatgtt ctaagagaaa agccagcgga ataatactgt atgacttttg ttcactagcc
     2401 gtgacgagtt cttcaatttc tcgtcttttt agtaataatt tgcgagtttt tttgggatca
     2461 taatcttggt t
//
LOCUS       DAOSEW010000086         2451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_41833_length_2451_cov_4.449917, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000086 DAOSEW010000000
VERSION     DAOSEW010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41833_length_2451_cov_4.449917"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="PLJ02_03285"
     CDS             <1..755
                     /locus_tag="PLJ02_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOI05461.1"
                     /translation="DEIIVVATPEGKPIYEDYFNNSVNRIRKQLAAQGKESRYVPVQE
                     VLNFPKIVVITQDSSLPYGNGSPFASAKNYVDTEEAFLAFYSDDVIFGKPGDAKTLIE
                     AYHRSPDADAIIMVKEVPEEEITKYAAVSLKNGQYREDQENILADIIEKPDPAQAPTH
                     LASYGRYLLTPKIFKFLSPQNVGKDEELWTADAIGKLNQSGGKVLVQKSRGEWMTTGD
                     PENYFKAHLKFVINHEKYGPKVASWLKEFSKE"
     gene            complement(742..1107)
                     /locus_tag="PLJ02_03290"
     CDS             complement(742..1107)
                     /locus_tag="PLJ02_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05462.1"
                     /translation="MKKLKKNSPSLLQIIASAIKLTKKSNAKPKKPQNPPPFAGNQRL
                     SPGEPGGQGENFSTEKFYNLSAHRKAVEEKNAIASCKRRWSFKVHFHSRKTNHKRTAV
                     VNSKNLLEWNISLLEAILY"
     gene            1141..1213
                     /locus_tag="PLJ02_03295"
     tRNA            1141..1213
                     /locus_tag="PLJ02_03295"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1174..1176,aa:Thr,seq:ggt)
     gene            1273..1354
                     /locus_tag="PLJ02_03300"
     tRNA            1273..1354
                     /locus_tag="PLJ02_03300"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1307..1309,aa:Tyr,seq:gta)
     gene            1360..1433
                     /locus_tag="PLJ02_03305"
     tRNA            1360..1433
                     /locus_tag="PLJ02_03305"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1394..1396,aa:Thr,seq:tgt)
     gene            1442..2326
                     /locus_tag="PLJ02_03310"
     CDS             1442..2326
                     /locus_tag="PLJ02_03310"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HOI05463.1"
                     /translation="MLTFLLKDLVLYISERNDGDFRQTQHIREFLQAQPAIGEDNFHL
                     LHLQLENKQQSIVLTNSELFSKQTTQIFVGDAILTNYKKGERETNQTLISMVVGDCFP
                     LIFFDQKSKNIAMIHAGWEPLDLGILDRVWQQMTSLWGVKTKNIWAFLGPGIRAQSYC
                     VASEPRQSQRPEWQQFIKKISFSQKAKGQQQNLDKGQQPNRAKDLHPNEEKEVWQIDL
                     PGFIKDFLTRKGLPQEQLFDSQLDTYQLPDQFFSHRRSQEQLAKGEKPLAQANDGRFL
                     VACQLSLPISASKNFLLT"
BASE COUNT          750 a          453 c          519 g          729 t
ORIGIN      
        1 ttgacgaaat tattgtggtg gctactcctg agggtaagcc aatttatgag gattacttca
       61 ataacagtgt caatcgtatt cgcaaacaat tagctgctca aggcaaggaa agtcgttatg
      121 tcccggtgca ggaagtactg aattttccca aaattgtggt aattactcaa gactcaagtt
      181 tgccttatgg taatggttca ccttttgcta gtgctaagaa ctacgtagat accgaagagg
      241 catttttggc cttttacagt gatgatgtga tttttggtaa gccaggtgat gccaaaactc
      301 tgattgaagc ctatcatcgt tctccagacg ctgatgcgat cattatggtg aaagaagttc
      361 cagaagaaga aattactaaa tatgctgctg tctctctaaa aaatggacaa tatcgggaag
      421 atcaagaaaa cattttagca gacattattg aaaaaccaga tccagctcaa gcaccaaccc
      481 atctagcatc ttatggtcga tatttattaa ctcccaaaat ttttaaattt ttgtcgccac
      541 aaaacgttgg caaagatgag gaattatgga cagccgatgc cattggtaaa cttaaccaaa
      601 gcgggggcaa agtcttagtc caaaaatctc ggggagaatg gatgaccact ggtgatccag
      661 aaaattattt taaagcccac ttaaagttcg tgattaatca tgaaaaatat ggtccaaaag
      721 tagccagttg gttgaaagaa tttagtaaag aatagcttct aacaagctaa tattccattc
      781 tagcaaattt ttgctattta caacagctgt tcttttgtgg ttagtctttc tagaatggaa
      841 atgtacctta aaactccacc ttcttttgca ggaagctata gcattttttt cctcaacagc
      901 ttttctatgg gctgagaggt tgtaaaattt ctccgtggag aaattttcgc cttgcccacc
      961 tggttcacca ggggaaagac gctggtttcc tgcgaaaggg ggtggatttt gtggtttttt
     1021 tggttttgcg ttactctttt tggtcaattt tattgctgac gctattattt gtaacagaga
     1081 tggactgttt tttttgagtt ttttcaatat aatagtgagg cattgtttgg ctaagtctta
     1141 gcctttgtag ctcagttggt agagcgcagt cttggtaaga ctgaggtctc gggtccgatc
     1201 cccgacaaag gctcatgatt tcggctaaat taaacaaagt gagcaaaaac gtgtaactta
     1261 acagcaattt gcgccaggat actcaagtgg tcaacgaggg cagtctgtaa aactgctggc
     1321 ttagcctacg taggttcgaa tcctgctcct ggcaccgtcg cccccctagc tcaattggtt
     1381 agagcacctg ttttgtaaac aggaggttca gggttcaagt ccctggggtg gctcagtttt
     1441 tatgctaact tttttactca aagatctcgt cttatatatc agtgagcgta atgatgggga
     1501 tttccggcaa acgcagcata tcagagaatt tttgcaggct cagcccgcaa ttggagaaga
     1561 taattttcat ttactacatc tgcaactgga aaataagcag caatccattg ttttgaccaa
     1621 cagcgaactt ttcagtaagc aaacaacgca aatttttgtt ggtgacgcta ttttaactaa
     1681 ttacaaaaag ggggaaagag agactaatca aaccctaatc agtatggtag ttggggactg
     1741 ttttccgttg atattttttg atcaaaaaag caaaaatatt gcgatgatac atgctggttg
     1801 ggagccacta gatttaggaa ttcttgatcg ggtttggcaa caaatgacca gtttatgggg
     1861 agtaaaaacc aaaaatattt gggctttttt ggggccagga attagagctc aatcttattg
     1921 tgtagcgagt gaaccgcgtc aaagtcagcg tccagaatgg caacaattca ttaaaaagat
     1981 tagttttagt caaaaagcca aaggccaaca gcaaaacctt gacaaaggcc aacagccaaa
     2041 cagagccaaa gatttgcacc caaatgaaga gaaggaagtt tggcaaattg atttaccagg
     2101 gtttattaaa gactttttaa ccaggaaagg cttacctcaa gaacaattgt ttgatagcca
     2161 gctggatact taccagttgc cagaccaatt tttttctcat cgacgcagtc aagaacaatt
     2221 agctaaaggg gaaaaaccat tggctcaggc aaacgacggt cgctttttgg tggcttgtca
     2281 actatctttg ccaatatctg catctaagaa ttttttactt acttaagtaa ttttcacttc
     2341 ttaatctttt gtgttaaaat actcccttgt tatcgcaaaa attttaagat aaaaaggaaa
     2401 acatggcaaa aacaaaaaaa ggtccccgac agccaattgg tcttcaatgt a
//
LOCUS       DAOSEW010000087         2313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_45626_length_2313_cov_2.906112, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000087 DAOSEW010000000
VERSION     DAOSEW010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45626_length_2313_cov_2.906112"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /gene="thyA"
                     /locus_tag="PLJ02_03315"
     CDS             <1..675
                     /gene="thyA"
                     /locus_tag="PLJ02_03315"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: protein motif:HMM:NF012524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOI05464.1"
                     /translation="IIAFINGARTIEELESYGCYWWKAWGTEDKAKKRHLKIGDLGPG
                     SYGAAFHDFPTAEGETYNQFAHVLQQMRENPHLRTHFITPWIPQYTMRIKGRTQKVVV
                     CPCHGWIHLRIIDNQLTLHMFQRSADLPIGVPSNMVQYAALTMMIAQVLDLEAYEFVH
                     SFSDAHIYVDQIEAVKTMLAREPKPLPHMKLNPQVKDLFAFRKEDFTLENYQPHPGIW
                     NIPVAV"
     gene            677..1147
                     /locus_tag="PLJ02_03320"
     CDS             677..1147
                     /locus_tag="PLJ02_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOI05465.1"
                     /translation="MTKKAKNQFINLANARETEQKSVMKDIADAKHCPFCRENLCKYH
                     QQPILKEGQFWLVTTNQWPYRHTKHHFLLIYKVHAVSLSDLSPAAGQELFELIAEIEQ
                     SYAIKGGGLAMRFGDTDYSAGTISHLHVQLIEPDIESPNFVPVRIKIGRNLTED"
     gene            complement(1187..2155)
                     /locus_tag="PLJ02_03325"
     CDS             complement(1187..2155)
                     /locus_tag="PLJ02_03325"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HOI05466.1"
                     /translation="MEKVLALYLPVLHEGYRLFLAKNSDAKKLLLFDQKLLRTWPAFA
                     YLKKDIRALDSQLMKKAVSQLFPQLDIIVINHQQDWQNLLNLAKDRRWQWVLSNDDLG
                     RYLAAEQLVGQPITFDSYFLRWDRLAVEQAQRQLSKQTDQAPSEEFQPDQIISQDSLE
                     KEIMKQAFVQANYSNNWWRQVGVVLLKDGQVLLSTYNQHLPNPQEQYFSGDPRSLFTS
                     GEAIELTSSIHAEALAIARAAKQGISLAGADLFVTTFPCPICAKQVAASGIKRLYYSQ
                     GYAILDGWQVLKAADIELIQVKFNQAEKDQLARQTSQASNLKKRYS"
BASE COUNT          689 a          476 c          445 g          703 t
ORIGIN      
        1 attattgctt ttattaatgg tgccagaacc attgaagaat tagaaagcta tggttgttat
       61 tggtggaaag cttggggtac tgaggacaaa gccaaaaaac gccacctaaa aattggcgat
      121 ttgggtccag gttcatacgg cgccgccttt catgacttcc ctactgctga aggtgaaacc
      181 tacaatcagt ttgctcatgt tttgcaacaa atgcgcgaaa acccccattt gcgcacccac
      241 tttattaccc cttggatccc ccaatacacc atgcgtatca aaggtcgtac acaaaaagtg
      301 gtggtttgtc cttgtcatgg ttggatccac ctgagaatta ttgataatca actcacttta
      361 cacatgtttc agcggagtgc tgatttgccg attggggtac catccaacat ggtgcaatac
      421 gctgctttaa cgatgatgat tgctcaggta cttgatttag aagcttatga attcgttcac
      481 agtttttctg atgctcatat ttatgttgat caaattgaag ccgtaaaaac catgctagct
      541 cgtgaaccca aacctctgcc acatatgaaa ttaaatccac aagtcaaaga tttattcgcc
      601 ttccgtaaag aagattttac gttagaaaat tatcaacccc atcctggaat ttggaatatt
      661 cccgtagcag tttaacatga caaaaaaagc aaaaaatcag tttatcaatc tagcgaacgc
      721 ccgagaaaca gaacaaaaat cagtcatgaa ggacattgcg gatgccaaac actgtccatt
      781 ttgtcgggaa aatctctgca aatatcatca acaaccaatt ctcaaagaag gtcagttttg
      841 gctggttact accaatcaat ggccatatcg ccacaccaaa caccattttt tattaatcta
      901 caaggtgcat gcggtgagtt tgagcgattt atcaccagct gctggtcaag aattattcga
      961 actaattgct gaaattgaac aaagttatgc gattaagggg ggtggattgg cgatgcgttt
     1021 tggcgatact gattatagtg ctggtactat tagtcattta catgtgcaat taattgagcc
     1081 agatattgaa agtcctaatt ttgtcccagt gagaattaaa attggtcgaa atctgactga
     1141 ggattaaata ttttttgaca tctagttgat ggaaaaacca aaaatttcaa gaataacgtt
     1201 tttttaggtt gctggcttgg ctggtttgtc tcgccaattg gtctttttct gcctgattaa
     1261 atttgacttg aataagctca atgtctgccg cttttaatac ttgccaacca tccaaaattg
     1321 cataaccctg actatagtaa aggcgcttaa tcccactagc tgctacttgt ttagcacaaa
     1381 ttgggcaagg aaaagtagtg acaaataaat cagcaccagc gagagaaatg ccttgtttgg
     1441 cggcgcgagc gatagcgagc gcctcagcat gaatagagct ggtgagttca atggcttccc
     1501 ctgaagtaaa taaactccgt ggatcaccgc taaaatactg ttcttgcggg ttgggtaaat
     1561 gttgattgta agtactgagt aaaacttgtc catctttgag taacacgacc ccaacttgcc
     1621 gccaccaatt gttactataa ttagcttgca caaaagcctg cttcatgatc tctttttcca
     1681 aactgtcttg actaatgatc tgatctggtt gaaattcctc agaaggtgct tggtctgttt
     1741 gtttgctcaa ttgtcgctga gcctgctcaa ccgccaatct gtcccaacgc aaaaaatacg
     1801 agtcaaaagt aattggttgc cctactaatt gctccgctgc taaatatctg cctaaatcat
     1861 cattactgag cacccactgc catcgtctat ctttggccaa atttaacaag ttttgccaat
     1921 cttgctgatg gttaatgacg ataatgtcta attgtggaaa aagctgactg accgctttct
     1981 tcattaattg actatcaaga gcccgaatat ctttttttaa ataggcaaaa gctggccaag
     2041 ttcgcaacaa tttttgatca aacaaaagga gttttttagc gtctgagttt ttagctaaaa
     2101 ataatcgata gccttcatgt aaaactggta aatataaagc taagactttt tccataacat
     2161 tattgtttaa tcatccttaa attctgccaa aacgtcctca atggtagcaa ttaatttact
     2221 agtcatttta tctttaatgc tattagcggt tttcatctgc attagtcgga ttttgcccgt
     2281 ggtactagta gtggccatcg gttccaaaac aac
//
LOCUS       DAOSEW010000088         2287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_46386_length_2287_cov_3.137993, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000088 DAOSEW010000000
VERSION     DAOSEW010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46386_length_2287_cov_3.137993"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PLJ02_03330"
     CDS             complement(<1..634)
                     /locus_tag="PLJ02_03330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOI05467.1"
                     /translation="MPKISYYNIDYQDVLNKLGSDREKGLSKNEVSKRQQQFGSNEIA
                     DDRPKHLVLKILWNQFNNLLVLILAISAIITYLLDNKLDTYVIIFAILSDAIIGFVQE
                     YRANKAIESLKEIVVATSKVYRDGQLIEIPAKNLVPGDIVLLEEGDRVPADGRLIESY
                     NLQVIESSLTGEPFAVEKNTEAIKNKTPLADRKNYVWMSTFVVSGFAKFVV"
     gene            727..>2287
                     /locus_tag="PLJ02_03335"
     CDS             727..>2287
                     /locus_tag="PLJ02_03335"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI05468.1"
                     /translation="MFKKIYEYTKPYRKTLILISVLIIVMALLNQVEPFITRAITDRL
                     VNNQDRGLFSYIAILLLVLLLVKLIQSVLNRLSWYLTNIFVIKFEASLKQLGFDHLMK
                     LSLSFYNEQETGKVMSELDRGVNRLTSIVNNSGMHFIPSVTTAILSFAIVLYYEWRIG
                     VITVLAFVPYVAINRWRFEKNNKLERREYHLYDKQYAHFWEVLSSMELIKSFRAEEFE
                     KRQLKDFFQKVINLRQEMEKNTNRSAIGDIILESLSWAMYAYIVYITWQGQITIGTLI
                     LLVGLIQMIRQPLWQINWIFWEVKRAQIGARDFFKIMNVKPEVVDPVKPVAINNLQGK
                     IVFDNVSFAYSKNMYRYFDKNDEDEDELKSLVKEQSHHNPKRLKVLDHVSFTIEPGQM
                     TAFVGPSGAGKSTIASLLMRFFDPDEGTIYLDGIDIKKFRQQDLRAQMGLVSQSAYLF
                     ASSIEENLRYAKPDATEEEMWRACKIARADEFIEKLDKGLKTTIGERGVKLSGGQRQR
                     LSLARTILRDPK"
BASE COUNT          712 a          391 c          425 g          759 t
ORIGIN      
        1 taactacaaa tttggcaaaa ccacttacta caaaagtgct catccataca taatttttac
       61 gatcagccag gggtgttttg tttttgattg cctcagtatt tttttctaca gcaaatggct
      121 cgccagttaa agaggattca ataacctgca agttataact ttcaatcagt cgaccatcag
      181 ctggcacccg atctccttct tctaataaaa caatatcgcc aggcaccaaa tttttggccg
      241 ggatttcaat taattgacca tcccgataaa ccttactcgt tgccaccaca atttctttga
      301 gtgactcaat tgctttattg gctctatatt cttggacaaa accaattatt gcatcagaca
      361 agatcgcaaa aataatgacg taggtatcaa gcttattgtc taaaaggtaa gtaataatgg
      421 ctgaaatcgc taggatcaag actagtaaat tattaaattg gttccagaga atttttagaa
      481 ctaaatgctt gggtctgtca tcagcgattt cgttgctacc gaattgttgt tgtcttttgc
      541 taacttcgtt tttacttaag cctttttctc gatcgctacc caatttattt aaaacatctt
      601 gataatcaat attgtaatag gaaattttgg gcatatgact actcagtgta gcaaaattgg
      661 tattatgaaa caagcttttc ctctagtttt tggccttgag agctgataaa ctatcgccat
      721 caagtaatgt ttaaaaaaat ttacgaatat accaagccct accgtaaaac actaatttta
      781 attagtgttt tgattatcgt gatggcgtta ctcaatcaag ttgagccatt tattactcgc
      841 gctattaccg atcgtttagt caacaatcaa gatcgagggt tgttttctta tattgctatt
      901 ttgcttctag tcttattact ggttaaatta attcaatcag tgctgaatcg tttgtcttgg
      961 tacctaacca atatttttgt gattaaattt gaagccagtt taaaacagtt gggttttgat
     1021 catttaatga aattatcact ctctttttat aatgaacagg aaactggcaa ggtaatgagc
     1081 gaactagatc ggggggtaaa tcgcttgact tcaattgtca ataattctgg catgcatttt
     1141 attcctagtg ttaccaccgc aatattatct tttgccatag ttctttatta cgaatggcga
     1201 attggcgtaa ttacagttct agcgtttgtt ccctacgtgg ccattaatcg ctggcgtttt
     1261 gaaaaaaata acaaattaga acgtcgcgaa tatcacctat atgataaaca gtacgcccat
     1321 ttctgggagg tattaagttc gatggaatta attaaatcat ttcgagcaga agaatttgag
     1381 aaacgccaat tgaaagattt tttccaaaaa gttattaacc tccgccaaga aatggaaaaa
     1441 aataccaatc gttcggccat tggtgacatt attttagaat cactaagttg ggcgatgtat
     1501 gcatatattg tttatattac ttggcagggg cagattacta tcggtacttt aattttatta
     1561 gttggcttaa ttcaaatgat tcgtcaaccc ttgtggcaaa ttaactggat tttttgggaa
     1621 gtcaagcgag ctcaaattgg agctcgagat ttttttaaaa ttatgaatgt aaaaccagaa
     1681 gtcgttgatc cagttaagcc agtagctatt aataatttgc agggaaaaat tgtttttgat
     1741 aatgtgtcgt ttgcttattc caaaaatatg tatcggtatt ttgataaaaa tgatgaagat
     1801 gaagacgaat taaaaagcct agtgaaagaa cagtcacacc acaatcccaa aagattaaaa
     1861 gttcttgatc atgtatcttt tactattgaa cctggtcaaa tgaccgcttt tgttggtcca
     1921 tctggggctg gtaagagtac tatcgccagt ttattaatgc gtttttttga tcctgacgag
     1981 ggaacaattt atttagacgg aattgatatt aaaaaatttc gtcaacaaga cttgcgagca
     2041 caaatgggct tagttagtca aagtgcctat ctgtttgcta gctcaattga agaaaatttg
     2101 cgctatgcca aacctgatgc tacggaagag gaaatgtggc gagcttgtaa aattgctcga
     2161 gctgatgaat ttattgaaaa attagataaa ggtttaaaga ctactattgg tgagcgaggg
     2221 gtaaaactct ctggtggaca gcgccaacgc ttatcgctag cgagaactat tttacgtgat
     2281 ccaaaaa
//
LOCUS       DAOSEW010000089         2258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_47233_length_2258_cov_3.851566, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000089 DAOSEW010000000
VERSION     DAOSEW010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47233_length_2258_cov_3.851566"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PLJ02_03340"
     CDS             <1..252
                     /locus_tag="PLJ02_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05469.1"
                     /translation="TPASKKKIIDHFQQCLNLREIDDKAIELLEILAVTHSQEIKNFW
                     RILGGPEKAIADVYECGVWSIELEADEIEFKWTEGDLNP"
     gene            complement(226..299)
                     /locus_tag="PLJ02_03345"
     tRNA            complement(226..299)
                     /locus_tag="PLJ02_03345"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(263..265),aa:Ala,seq:ggc)
     gene            complement(911..1921)
                     /locus_tag="PLJ02_03350"
     CDS             complement(911..1921)
                     /locus_tag="PLJ02_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOI05470.1"
                     /translation="MNAQENFSKAQTAKTQASAKLNKAVSHLSTDPDQALSAFLESLE
                     VEKNLSRLTIRNYKHYLNRFNNWLKEQGATDLTQLDQDLLRAYRVFLARYSDEQGRTL
                     SKRTQSYHVIALRSWFKYLVKNDLAVLHPEKIDLPKAESTPMNFVGIEEMERLLSAPE
                     LNSKTGLRDRAILEVLFSTGLRVSELVKLNRDQINLKRREFGVIGKGRRPRVVFLSER
                     AVDWLSRWLATRNDMWQPVFIRLSGKKIHPAFDGEEMRLSSRSVQRLVDKYSRKAHLT
                     VKLSPHGIRHSFATDLLINGAGLRDVQEILGHKNIATTQIYTHVTKSELKKVYDKRHS
                     KL"
     gene            complement(1989..>2258)
                     /locus_tag="PLJ02_03355"
     CDS             complement(1989..>2258)
                     /locus_tag="PLJ02_03355"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006719070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOI05471.1"
                     /translation="GGHIGYVIRPSARRQGYGSQLLALALEKIRQGQPSLDTDQALLT
                     CDENNLGSKKIIEKNGGIFSGYSEQEAPLPRKLLYWVPYNLPKIG"
BASE COUNT          659 a          438 c          435 g          726 t
ORIGIN      
        1 acgccagcca gtaaaaagaa aattattgac cactttcaac aatgcctcaa tctcagagaa
       61 attgacgaca aagccatcga actgttagag attttggctg tcactcatag ccaggagatt
      121 aaaaattttt ggcggatctt gggtggacca gaaaaagcca ttgccgatgt ttatgagtgc
      181 ggggtgtgga gcattgagct ggaagctgat gaaatagaat ttaagtggac cgaaggggac
      241 ttgaacccct gacctcttct atgccataga agcgctctag ccaactgagc tatcggcccg
      301 tttttaactt tttgattcta acattgaacg aaataatttt aaagtaagag tccattggtg
      361 agttttgcat gttattttta tatataagta ggcatttgat atattaaaaa aataaacttt
      421 aggctctttg atctattact atattttaat atgttaatta ctatattttg attgattatt
      481 tttatcactt gtttgccaaa tttttcctaa taaacaaaat tgcgattgtt tagactgtgt
      541 taaattgctg gctaattctc aagacgacta gtttttttta taaattaaat cactgatttt
      601 ccttgagatc tatacagctc caacgctcaa cttaatataa aaataatatt tttagaaaga
      661 ttaaatcttt ttcaagaaaa aattacatac tttaaagtgt ttttcataaa atttgctggc
      721 taaaacctta tggacaatta gcagactgat caaatgactg ctaaaaagga ctttacaagc
      781 tttttaccca tgtatcaaga taattttagt caatttttcg attaagctgt tttttgcctc
      841 aaataatttt tttaaagaaa aggacgttgt actcgagtag gcgccattgg ctaattttgc
      901 ttttttacta tcacagtttg gaatggcgct tgtcgtaaac tttttttaat tcagatttag
      961 taacgtgagt atagatttgg gttgtcgcaa tatttttatg acctaaaatt tcttgtacat
     1021 cccgtagtcc cgcaccatta attaacaagt cggtggcaaa actatgacgg ataccgtgag
     1081 gcgaaagttt aacagttagg tgcgcttttc tagaatattt gtctactaag cgctggacac
     1141 tgcgtgaact taatcgcatc tcctcaccat caaaggcagg atgaattttt ttacctgaca
     1201 ggcgaataaa aacgggttgc cacatatcat tgcgggtagc tagccagcgg ctcaaccaat
     1261 cgacggctcg ctcacttaaa aagactaccc tgggtcgccg acctttgcca atcaccccga
     1321 attcacgtct ttttaaatta atttgatcac gattaagctt gaccaactca ctaacacgca
     1381 aaccagtaga gaagagtact tccaaaatcg ctcggtcgcg caaaccggtt ttactgttta
     1441 gctctggcgc gcttaataac cgttccattt cttcaatacc aacaaagttc atgggagttg
     1501 attcagcctt gggtaaatca attttttctg ggtgaagcac cgccaaatca tttttgacta
     1561 aatatttaaa ccaagagcgc agagcgatca catgataact ctgggttctt ttggacaagg
     1621 ttcgaccctg ttcatcgcta tagcgagcca aaaagacgcg ataagcgcgc aataagtctt
     1681 ggtcaagctg ggttagatct gtcgctcctt gttccttcaa ccagttgttg aaccgattta
     1741 aatagtgctt gtaattgcga atagttaaac ggcttaagtt tttttctacc tcaagtgact
     1801 cgaggaaagc tgacagtgcc tgatctggat cagtactcag gtggctaaca gctttgttta
     1861 gtttggctga tgcttgagtt ttagctgttt gcgctttact gaaattttct tgagcgttca
     1921 tatcctcaat ttacaataaa attagacttt tggccaccag cgactctgat ttaagcaaat
     1981 agggtagact accctatttt tggcaaattg taggggaccc agtagagtaa ttttcgaggt
     2041 agtggcgctt cttgctcact ataaccacta aaaatgccac catttttctc aataatcttt
     2101 tttgaaccga gattattttc atcacaggtg agtaaggcct gatcagtatc caagctgggt
     2161 tgtccttggc ggattttctc tagcgcgagg gctagtaatt gagagccata accttgacga
     2221 cgcgcactcg gtctaatcac gtaaccaata tgaccacc
//
LOCUS       DAOSEW010000090         2237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_47873_length_2237_cov_4.079743, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000090 DAOSEW010000000
VERSION     DAOSEW010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47873_length_2237_cov_4.079743"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1793
                     /gene="fusA"
                     /locus_tag="PLJ02_03360"
     CDS             <1..1793
                     /gene="fusA"
                     /locus_tag="PLJ02_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOI05472.1"
                     /translation="DGGITVLDASEGVQSQSETVWRQADKYNVPRICFVNKMDKLGAD
                     FKATVEDIRTKFGVKPAVMVLPMGSEDHFAGVIDLLTRRAYFWEDHQAATEPDIKDIP
                     EEFQTEVESLRAELVESIAETDDVLMEKFLNSQEIAVDELKAALRRAVIDYKLVPVYA
                     GSSLKNTGIQPLLDAVIEYLPSPLDIDHISGLKPGTEEVIERKLTPEEKFCGLAFKIQ
                     TDPHVGKLTYFRIYSGVLKSGTYVYNSSKNVKERLGRLLLMHANQREEIEEAAAGEIV
                     AMIGLKETKTGDTLCDENYPIILEGITFAEPVIYLAIEPRTKADQEKMGMALAKLSEE
                     DPTFSIKSDPETGQTIIGGMGELHLEIIVDRLKREFHVDADVGKPQVAYRETIKQMAE
                     GEGKYIKQSGGRGQYGHVLLRIEPKERGTGYEFVNEIKGGTVPREYIEPTNKGVQEAL
                     ERGFLANYPMVDIRVTLYDGSYHEVDSSELAFKMAGSFAMRDAVSKADMVLIEPIMKV
                     EVSTPEEFMGDVIGDLGSRRAQIQGTNKRGNISIITALAPLAEMQGYVTTLRGLTQGR
                     ATSVLLPSHYEEVPRNITEAIIEERKGKKA"
     gene            1867..>2237
                     /locus_tag="PLJ02_03365"
     CDS             1867..>2237
                     /locus_tag="PLJ02_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015473857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOI05473.1"
                     /translation="MADTQKFVRNKPHVNIGTIGHVDHGKTTLTAAITHVLAEKYPSD
                     INKALNYEDIESSAEEISRGITINISHVEYETEKRHYAHIDAPGHADYIKNMITGAAQ
                     MDGAILVVSATDGPMPQTREH"
BASE COUNT          720 a          360 c          509 g          648 t
ORIGIN      
        1 tagatggggg cattactgtc ctcgatgcta gtgaaggtgt gcaatcacaa tcagaaactg
       61 tttggcgaca agctgataaa tataatgttc cgcgaatttg ttttgtcaat aaaatggata
      121 agttaggagc tgattttaaa gctactgttg aggatatccg caccaaattc ggagtcaaac
      181 cggcagtaat ggttttacca atgggtagcg aagatcattt tgctggagtg attgatttat
      241 taactcggcg agcttatttt tgggaagatc atcaagcagc gaccgagccc gacatcaaag
      301 atattccaga ggaatttcaa acagaagttg aaagcttacg cgcagaatta gtggaatcca
      361 ttgccgaaac agacgatgtg ctaatggaaa aatttttaaa ttcgcaagaa attgcagtag
      421 atgaattaaa agcggcactg cgtcgagcgg taattgatta caaattggtt ccagtttacg
      481 ctggttcatc actaaaaaat actggaatac aacctttatt agatgcagtg attgaatatt
      541 tgccatctcc attagacatt gatcatattt caggtttaaa gcctgggact gaagaagtca
      601 ttgaacgtaa attaacgcct gaggaaaaat tttgtggtct ggcttttaaa attcagactg
      661 atcctcacgt cggtaaatta acatattttc gtatttattc aggtgttttg aaatcaggta
      721 cttatgttta taactctagt aaaaatgtta aagaaagact gggtcgatta ttattgatgc
      781 acgccaatca aagagaggaa attgaggaag ccgcagctgg ggaaattgta gccatgattg
      841 gtttaaaaga aactaaaact ggcgacactc tatgtgatga aaattacccc attattctag
      901 aggggattac ttttgctgaa ccagttattt atttggctat tgaacctcga accaaagctg
      961 atcaagaaaa aatgggaatg gctttggcta aattaagtga agaagatccc acttttagta
     1021 ttaaatctga tccagaaact ggccaaacga ttattggtgg catgggtgaa ctacatttgg
     1081 aaattattgt tgatcgttta aaaagagaat ttcatgttga tgctgatgtc gggaaacccc
     1141 aagtggccta tcgagaaact attaaacaaa tggcagaagg tgaaggtaag tacattaaac
     1201 aatctggtgg tcgtggtcaa tacggtcacg ttttgctacg aatcgagccc aaggaacgtg
     1261 gcactggtta tgaatttgtt aatgagatta agggtggaac tgttcctcga gaatatattg
     1321 aaccaaccaa taaaggtgtt caagaagcgt tggaacgggg atttttggcc aattatccga
     1381 tggtcgatat tcgagtaact ttgtatgatg gttcttatca cgaagtggat tctagtgaat
     1441 tagcctttaa aatggctgga tctttcgcta tgcgtgatgc tgttagtaaa gccgacatgg
     1501 ttttaattga accaattatg aaagttgaag tgagtacacc agaagagttt atgggagatg
     1561 tgattgggga tttgggtagt cgtcgagcac aaattcaagg tactaacaaa cgtggcaata
     1621 ttagtattat tacagctctc gctccattag cagaaatgca gggttacgta acaactttgc
     1681 gtggccttac tcagggtcgg gctactagtg ttttgttacc cagtcactat gaagaagtac
     1741 cacgtaatat tactgaagcg attattgaag aaagaaaagg taagaaagcc taattttttc
     1801 gcttagtgta taataatagg gtcaaaaaaa ttattaataa gtattatcaa aaaggagata
     1861 caagttatgg cagatacaca aaaatttgtc agaaataagc ctcacgttaa tattgggact
     1921 attggtcacg tcgatcatgg taaaaccact ttaactgcag cgattaccca tgtgttagca
     1981 gaaaaatatc cttcggatat taataaggcg ttgaactacg aagatattga gagcagtgcc
     2041 gaggaaattt ctcgaggtat tactattaat atctctcacg ttgagtatga gactgaaaaa
     2101 cgtcactatg ctcatatcga tgccccaggt cacgctgatt atattaaaaa catgattact
     2161 ggtgctgccc agatggatgg agcaattttg gtagtgtcag cgactgatgg tccaatgccc
     2221 cagactcgtg agcacat
//
LOCUS       DAOSEW010000091         2216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_48577_length_2216_cov_3.315132, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000091 DAOSEW010000000
VERSION     DAOSEW010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48577_length_2216_cov_3.315132"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(205..597)
                     /locus_tag="PLJ02_03370"
     CDS             complement(205..597)
                     /locus_tag="PLJ02_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05474.1"
                     /translation="MSKKQVISLGVVSLLTAGIFLLPGAADAYRGDPSLEGPNCTAER
                     HEAMTAAFANNDYSAWQEQMNGKGRVAEVVNEGNFARFAEAHQLAEEGKVEEAEQIRA
                     ELGLGLGNGAGEGQGTGEGQGQMKRYNR"
     gene            complement(833..1102)
                     /locus_tag="PLJ02_03375"
     CDS             complement(833..1102)
                     /locus_tag="PLJ02_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05475.1"
                     /translation="MANHEQLSLERFSHLCRKYDVPLQLISAIQEEADPIKREQLCNE
                     VGRFLISAMCSFRIENSDANDVRIAAGLDGDENIAHNIIFPSKIR"
     gene            1245..2156
                     /locus_tag="PLJ02_03380"
     CDS             1245..2156
                     /locus_tag="PLJ02_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05476.1"
                     /translation="MEIRKRLKEDQELANYNSVIAHKVQMDSLTYDIISNLNEAPELR
                     GLDRQQVFQDAYQAVSKDYFAKASKLSQLYFSLLGNKKAIFLNNDDFVTLTNAYSGES
                     NTQSSYAMTLRKGLILFNQELITNEVADDKLVNIYQQMQGEKFNLPADQSQMIEFLAN
                     FIPFHCKMTDRSLDKIFWPKETVAAEELTTEILENTLWSMIYHECLHILSSSNSFLEN
                     LDEAATHYHTALATYLEKGPSAFGLSLILAGTNESITWINFLRDFDIEPQLAEEMYFN
                     QTGQWTAENILGLFPDKQQELSLLISG"
BASE COUNT          699 a          454 c          365 g          698 t
ORIGIN      
        1 aatcaagtat cgatatagct cttgatcttc atctctgacc aggcccacca gttcctcatc
       61 agttaatttt tggtaattgc tcataactat tgattaatcg ctattataaa gctcattgtt
      121 gactataact aatatatttt tcaatctagt taaaaagtaa aaaaagccca cttaaataat
      181 taagggggcc ttttttaaaa ccgtttagcg attgtaacgc ttcatttgac cttggccttc
      241 accagtgccc tgaccttcgc cagcgccatt accaagtccc aaacccaact ctgctctaat
      301 ttgttcagct tcctcaactt taccttcttc ggctaattga tgagcctctg caaatctggc
      361 aaaattacct tcgttcacca cttccgctac tcgaccttta ccattcattt gttcttgcca
      421 agctgaataa tcattattgg caaaagcagc tgtcatagcc tcgtgtcgtt cagcagtaca
      481 atttggaccc tcaagactag gatcaccacg ataagcatca gcggcgcctg gtagtaagaa
      541 gattccagca gttaataaac tgaccactcc tagggaaatt acttgctttt ttgacattgt
      601 tacctccttt ctgtttacaa cttttatcta actgtaatac tagaaagagg taatttttct
      661 ttcacgcaat tttttttctt aaggaaattt gcggattaag gcttaatctt ccagctacca
      721 aaccacgtct aacttgggtt tttttgcttt cggcttggct ggattattga caattttccc
      781 cagacgtaaa tcttattatt tatatatcta agatttgtgt agaaaatctc agctatctaa
      841 ttttacttgg aaaaataata ttatgagcaa tattttcgtc tccatcgaga ccagcagcta
      901 ttctgacatc attagcgtca gaattttcaa tgcgaaaact acacatcgct gagataagaa
      961 atctgccaac ctcattgcac aattgctctc tcttgattgg atcagcttct tcttggatag
     1021 ctgaaattaa ttgtaacgga acatcatatt tcctacataa atgagaaaat ctttcaagac
     1081 ttaactgttc gtggtttgcc attattcctc ctttaatttc caaagacgat tgataaaaat
     1141 aacgggggca atatagcaca aaaaagataa atgctcaaat tgagttaatc aactaatact
     1201 actctattag gcattattgc cttataattc aattaaaact ggttatggaa attagaaaaa
     1261 ggttaaaaga agaccaagaa ctagctaatt acaatagtgt aatagctcat aaagtacaaa
     1321 tggactcgct gacctatgac ataattagca atttaaatga agcgccagaa ttacgtggtt
     1381 tagatcgaca acaggttttt caagatgcct accaggccgt gtccaaagac tattttgcta
     1441 aagccagcaa actatctcag ctgtattttt cattactagg caacaaaaaa gccatttttc
     1501 tcaacaatga tgattttgta actttgacta acgcttatag tggtgaaagc aatacccaat
     1561 cgagttatgc gatgacttta agaaaggggc tgattctttt taatcaagaa ctaatcacca
     1621 atgaagttgc tgatgacaaa ttagtcaaca tttatcagca gatgcaagga gaaaaattca
     1681 atttgccagc tgatcaaagt cagatgattg aatttttggc caattttatt ccgttccatt
     1741 gcaaaatgac tgatagatcg cttgacaaga tcttttggcc aaaagagaca gtagccgctg
     1801 aagagctaac aacggaaata ctagaaaata ctctctggtc aatgatttat catgaatgtt
     1861 tgcatattct gagcagtagc aactcttttc tagaaaacct cgacgaggca gccactcatt
     1921 atcacaccgc cttggctact tatctggaaa aaggtccgag tgcttttggc ctatcactta
     1981 ttctcgctgg caccaatgaa agtattactt ggataaattt tttaagagat tttgacattg
     2041 aaccacagct agctgaagag atgtatttta accaaactgg tcaatggact gctgaaaata
     2101 tattgggcct tttccctgat aaacaacaag aactctcact acttataagt ggttgatatt
     2161 cccctatttt aagcccttat atgggacaaa gaggctagtt tcatcacacc agcaaa
//
LOCUS       DAOSEW010000092         2162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_50351_length_2162_cov_4.052682, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000092 DAOSEW010000000
VERSION     DAOSEW010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50351_length_2162_cov_4.052682"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1833)
                     /locus_tag="PLJ02_03385"
     CDS             complement(<1..1833)
                     /locus_tag="PLJ02_03385"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOI05477.1"
                     /translation="MNILIPHSWLSEHLSGQLKPKDLQKYLSLSGPAVERFQEVKLAD
                     GSKEIVYDIEVTTNRVDMMSIEGIARETATILTRAGFSIRRLKKDWPAVKQQSAVRLP
                     LPEIIYETDHIRRVMAVVLTDIKQVASPQYMQNRLQAVEINVHDAVVDITNYVTHELG
                     HPCHVFDYDKLMKMGGKIIIKEAKPGKKFVTLDEKEHHTVGGEIIFESATGEIIDLPA
                     VKGTLNTGIDHTTKNILFWIESIDPYKVRYASMAHAIRTQAAQLNEKKVDPHLAERVL
                     RYAVDLFEKLVKAKVASAVYDYFPDRQQGKQIVVDSQTIHDYLGIDLPSMDIAKILRD
                     LDCQVKIIQQKEGEQFVVIAPTYRADLEIAADIIEEIARIYGYHKLPSQLMTGALPTD
                     RPYQKLFDLEKAIKNFLANIGWQEIYSYSLVSEAEALMAGYPLNKQLKLQNPLKEENT
                     YLRRSILPSLISILQNANQSENWQLFEIAKLYHPQKNNLPKQEYYLAMISRRDFRQVK
                     GDLELLLNHFYVLKKKEIELLVEEIMTKNHSIYQQSAKLVIKKDQERILLGYLHVFAN
                     QMIGIEIKLNNLLKVAHNHPQYQKTNPHSLIIEQLTFTLRKQVAI"
     gene            complement(1872..>2162)
                     /locus_tag="PLJ02_03390"
     CDS             complement(1872..>2162)
                     /locus_tag="PLJ02_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013059418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOI05478.1"
                     /translation="SFEVDFSCQICGGTGYVKDENNKKVNCRFCKSGWHEVGGAGMVH
                     PNVLTAGGIDSKKYRGFAFGWGVERSYTLKQGLNLDDIRLFYSAERDFLEQF"
BASE COUNT          613 a          428 c          413 g          708 t
ORIGIN      
        1 gatggccact tgtttcctta gggtaaaagt aagttgttca ataatcagac tatgaggatt
       61 agttttttga tattgtggat gattatgagc cacctttaat aaattattga gtttaatttc
      121 aatgccgatc atctggttgg caaaaacatg taggtagcct aacaagattc gttcttgatc
      181 ttttttgatg accaatttag cgctttgttg ataaatgcta tggtttttgg tcataatttc
      241 ttccaccagc aattcgattt cttttttctt tagtacataa aaatgattga gtaataattc
      301 taaatcgcct ttgacttggc gaaaatctct gcgactaatc atggctaaat aatattcttg
      361 tttgggtaag ttattttttt gtggatgata aagtttagca atttcaaaca attgccaatt
      421 ttcactttga ttagcatttt gcagaatcga aatgagtgaa ggcaagattg aacgacgtaa
      481 ataggtattt tcttctttaa ggggattctg taatttgagc tgtttgttta agggataacc
      541 agccatcaaa gcttctgctt cactgactag cgaatagctg taaatttcct gccagccgat
      601 attagccaaa aaatttttaa tagctttctc caaatcaaaa agtttttggt agggacggtc
      661 ggtgggtaag gctccagtca ttaattgact aggcagtttg tgataaccat aaattcgagc
      721 gatttcttca ataatgtcag cagcaatttc caagtcggca cgataagtgg gcgcaatcac
      781 tacaaactgt tctccctctt tttgttgaat aatttttact tgacagtcta aatcacgcaa
      841 gattttggca atgtccattg acggcagatc aatacccaag taatcatgaa tagtctggct
      901 gtcgactacg atttgcttac cctgttgtcg atcgggaaaa tagtcataaa cggcgctggc
      961 cactttggct ttcactagtt tctcaaataa atcaacagcg tagcggagaa cgcgctcagc
     1021 taaatgggga tcaacctttt tttcattgag ttgagcggct tgggtgcgaa tagcatgagc
     1081 catagaagcg tatctcactt tataaggatc aatggattcg atccaaaaca gaatattttt
     1141 ggtggtgtga tcgatgccgg tattgagagt gcctttaacc gctggtagat caataatttc
     1201 cccggtagcg ctttcaaaaa tgatttcacc gccaacagtg tggtgttcct tttcatctaa
     1261 agtgacaaat tttttcccgg gtttggcttc tttaatgatg attttgccac ccatcttcat
     1321 cagtttgtca taatcaaaaa cgtgacaagg atgacccagt tcgtgagtca cataattggt
     1381 aatatcaacc acagcgtcat gaacattaat ctctacagct tgcagacgat tctgcatgta
     1441 ttggggacta gcgacttgtt taatatcagt taatactacc gccatcactc ttctgatgtg
     1501 gtcagtttca taaataattt caggcagtgg caaacgaacc gctgactgtt gtttaactgc
     1561 tggccagtct ttttttaatc tcctaatgga aaatcctgcc ctggttaaaa tagtggctgt
     1621 ttcacgagcg ataccttcga ttgacatcat gtcgacgcga tttgtggtca cttcaatgtc
     1681 gtagacaatt tctttgctac catcggctaa tttgacctct tgaaatcttt ccaccgcagg
     1741 accactcaaa gataagtatt tttgcagatc tttgggcttc agttggccag ataaatgttc
     1801 gctgagccag ctatgtggaa ttaaaatgtt cattcatcct ttatttgtgc atttttatga
     1861 ttcaatatta attaaaattg ctctaaaaaa tcccgttcgg cagaataaaa aagacggata
     1921 tcatcaagat tcaggccttg tttgagagtg taactgcgct ccactcccca accaaaagca
     1981 aaaccacgat attttttgct atcaattcca ccagccgtta acacattggg atggaccatg
     2041 cctgctcccc cgacttcgtg ccaaccagat ttgcaaaaac gacaattaac ctttttatta
     2101 ttttcatctt tgacataacc ggttccccca caaatttgac aactgaaatc aacttcaaac
     2161 ga
//
LOCUS       DAOSEW010000093         2156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_50547_length_2156_cov_4.850547, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000093 DAOSEW010000000
VERSION     DAOSEW010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50547_length_2156_cov_4.850547"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="PLJ02_03395"
     CDS             complement(<1..381)
                     /locus_tag="PLJ02_03395"
                     /inference="COORDINATES: protein motif:HMM:NF013030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05479.1"
                     /translation="MQIHPAILTDSLATFAQQLDLVKQVPTIKVIQVDIVDAVFTDNI
                     TINPTDLVDFDFDDLAIDFHLMTEEPMDYVWELIDCGQKLPTQSVIGQIEKMSYQLDF
                     LEEIKKAGWQAGLAINLFTPLENIE"
     gene            complement(388..822)
                     /locus_tag="PLJ02_03400"
     CDS             complement(388..822)
                     /locus_tag="PLJ02_03400"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HOI05480.1"
                     /translation="MKIYIASDHGGFKLKEEIKDSIEEPVFDLGPQQLDPADDYPQFA
                     FRVAEKVSQEPGSIGLLFCRSGSGMAIAANKVKGIRAVDVYNQQIAKHAITDNHANVF
                     AFGADYLTKEEVLACLDTILHTQADQDERHLRRIQQIIAYEK"
     gene            complement(915..1115)
                     /locus_tag="PLJ02_03405"
     CDS             complement(915..1115)
                     /locus_tag="PLJ02_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009058754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05481.1"
                     /translation="MAAFSYTNKKGQTYYLHTRDVTLKNGRVQTIYFFARDIRDGSLS
                     EVPAGYMVVETTRTGMPVLKKK"
     gene            complement(1253..1750)
                     /locus_tag="PLJ02_03410"
     CDS             complement(1253..1750)
                     /locus_tag="PLJ02_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05482.1"
                     /translation="MQEKFKKVVPYIFPILAFAFVVFMFVRWYSSRVAEQGNGLLSSD
                     LEITNLTQEEEESIIRGTTDFATVEMTAVGEASGEIRYQLTEDKLNFTVTANLPESDE
                     AFAVWLKEVDGDAKKKVFTLTYSKAGFIGSASVPAEILPVEVVVSTESDLLLEDALLR
                     GIIEK"
     gene            complement(1865..>2156)
                     /locus_tag="PLJ02_03415"
     CDS             complement(1865..>2156)
                     /locus_tag="PLJ02_03415"
                     /inference="COORDINATES: protein motif:HMM:NF014041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF84 family protein"
                     /protein_id="HOI05483.1"
                     /translation="QELWSTVWISVLDQAGQIYSASGARFRLPQVLADGILAGKELGE
                     VMGALFNDPNLKQKSGAIGVLTKNFIDRTEEYVAIAKLAIGLWYGRDWQAQI"
BASE COUNT          700 a          473 c          337 g          646 t
ORIGIN      
        1 ttcaatattt tctagtggcg taaaaagatt aatggctaaa cctgcctgcc aaccagcttt
       61 tttgatctct tccagaaaat ctaattggta agacattttt tctatttgac caatcactga
      121 ttgggttggt aatttttgac cacaatcaat taactcccac acataatcca ttggctcttc
      181 cgtcattaaa tgaaaatcaa ttgctaaatc gtcgaaatca aaatctacta aatccgtagg
      241 gttgatggtg atattatcag taaaaaccgc gtcaacaatg tctacttgaa tcactttgat
      301 ggttggcact tgtttgacta aatccaattg ctgagcaaaa gttgccaaac tatcagttaa
      361 aatggcggga tgaatttgca tagttattta tttttcgtaa gcaataattt gctgaatcct
      421 tcttaaatga cgctcatctt gatccgcttg agtatgcaaa atagtatcca gacaagcgag
      481 aacttcttct ttagttaaat aatctgcacc aaaagcaaaa acattggcat gattatcagt
      541 gatggcatgt tttgcaattt gttgattata aacatcaact gctctaatac ctttaacttt
      601 attagcggcg attgccatac ccgaaccaga ccgacaaaat aacagtccaa tgctaccagg
      661 ctcttgacta actttttcag ctacgcgaaa agcaaattgt gggtagtcat ccgctggatc
      721 taattgttga ggaccgagat caaacactgg ttcttctatg ctgtccttaa tttcttcttt
      781 gagtttaaac ccaccgtgat cgctagcaat ataaattttc ataaacatat cataacaaag
      841 aattagtaaa agctataaat aaaaaaatcc cgccttagta aaaaggcggg atcttaattg
      901 acaaatgaga aaaattattt tttcttcaat actggcatgc cagttcgagt agtttcaacc
      961 accatataac cggctggaac ctcactcaaa gaaccgtcac ggatatctcg tgcaaagaaa
     1021 taaatagttt gcacccgacc atttttcaag gtcacatcac gagtgtgaag atagtaggtc
     1081 tgaccctttt tgttggtata gctaaaagct gccatacaag tctccttctt cctggcccca
     1141 ggccagaata actgttaatt actaactgct aggttagcat acttgataag tatatcaaga
     1201 agcaatttta atttttaact atcaaaatat atcaaattaa ctcaatattt ctttactttt
     1261 caatgatacc gcgcaaaaga gcatcctcaa gcaataaatc agactcggta ctaactacta
     1321 cctcaacagg cagaatctca gccggcactg aagctgaacc tatgaatcca gccttagaat
     1381 aagtcaaagt gaaaactttt ttcttcgcgt cgccatcaac ttcttttaac caaacggcaa
     1441 aagcctcatc actctctggt aaattagctg taaccgtaaa atttaattta tcctccgtca
     1501 attgataacg gatttcacca ctggcttcac caacagccgt catttctacc gttgcaaaat
     1561 cggtagtccc acggatgata ctttcctctt cttcttgagt taaattagta atctctaaat
     1621 cactagataa taaaccatta ccttgttcgg ccactcgact agaataccag cggacaaaca
     1681 taaagaccac aaaagcaaaa gctaaaattg gaaaaatata gggaacaact tttttgaatt
     1741 tttcttgcat gagacctttg gcatcactaa taactgttat attatgatcc tattttgaag
     1801 tagaaataat aagctgtcaa gatcagttta tttttacatt ataggtttaa aattcgtgga
     1861 cagctcaaat ttgagcttgc caatctctcc cataccacag gccaattgct aacttagcaa
     1921 tcgccacata ttcctctgtc cgatcaataa agtttttagt taaaacacca attgctccac
     1981 tcttttgctt gagattggga tcattaaaca aagcacccat tacctcaccc aactctttcc
     2041 cggctaaaat cccatccgct aaaacctggg gaagacgaaa acgggcacca ctcgccgaat
     2101 aaatctgccc agcctgatct aagacgctaa tccaaactgt tgaccacaat tcttgg
//
LOCUS       DAOSEW010000094         1864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_63146_length_1864_cov_3.640133, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000094 DAOSEW010000000
VERSION     DAOSEW010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63146_length_1864_cov_3.640133"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PLJ02_03420"
     CDS             <1..269
                     /locus_tag="PLJ02_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05484.1"
                     /translation="DSDRRALYDFVVFVLAILIEEGDLSKIEREGWRLYSPQELAKID
                     QKEFRKATLAVLDFLPAALLFLADEGKIPQEEMLSILAERDASI"
     gene            288..1088
                     /locus_tag="PLJ02_03425"
     CDS             288..1088
                     /locus_tag="PLJ02_03425"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HOI05485.1"
                     /translation="MKKKLTLIKLGGSIITDKEVFMSLRKATLSRLVGEIASARQENP
                     QQMLIIGHGQGGFAHVPATKYQTMAGFINEESRLGMAVVQDTAATLNRLVVHEFIKHE
                     LPAVSFYASNTIVADQREKLHFCPDVVKEYLDKDLLPITCGDVLVDRQQGCTIWSTEK
                     ILAFLADYFLQQGVKVNKIVHVTEVAGFLDKQGQLVPEITAKTWPKFRQDLRHTRGFD
                     VTGGMGLKIEESLNLAKLGIESVIISGMLKNNLYWALTNQDCVGTTIR"
     gene            complement(1078..1500)
                     /locus_tag="PLJ02_03430"
     CDS             complement(1078..1500)
                     /locus_tag="PLJ02_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05486.1"
                     /translation="MKLLGRLIKLIIKLIIIVLILLAISIFLYQKGWLKTHPLQAQIN
                     QYQDGEINSWQDLVNSFHSDQKLNLELNELSTNAGEQIKSSAQKAIEAGKFAQEFVSE
                     SVQTNESTDKNLGERAFEYGQYVYCKAVVDDWEAKTNE"
     gene            complement(1581..1654)
                     /locus_tag="PLJ02_03435"
     tRNA            complement(1581..1654)
                     /locus_tag="PLJ02_03435"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1618..1620),aa:Ile,seq:tat)
BASE COUNT          572 a          315 c          389 g          588 t
ORIGIN      
        1 ccgatagtga tcggcgagcc ttatatgatt ttgtggtttt tgttttagca attttgatcg
       61 aagaagggga tcttagcaaa attgaaagag agggctggcg gctttattct ccccaagaat
      121 tagccaagat tgatcaaaaa gaatttcgta aggccacttt agctgtgctg gattttttgc
      181 cagcagcttt attgtttttg gctgatgaag gtaaaattcc tcaagaagag atgcttagca
      241 tactggctga gcgggatgca tcaatataaa ttacgagaag ataaatcatg aaaaaaaaac
      301 taactttgat taaattgggt ggttccatca ttactgataa agaagttttt atgtcattga
      361 gaaaagcgac tttgagtcgc ctagttgggg aaattgccag tgctaggcaa gaaaatcctc
      421 agcaaatgtt aatcataggt catggtcaag gaggctttgc tcacgtgcca gctaccaaat
      481 atcaaacaat ggccggtttt ataaatgaag aaagtcggtt agggatggct gtagttcagg
      541 acactgcagc aacacttaat cgtttagtcg ttcacgagtt tatcaaacat gaattaccag
      601 ccgtatcttt ttacgcaagc aacactattg tcgccgatca aagagaaaaa ttacattttt
      661 gtccagacgt agtgaaagaa tatttagata aagacttatt acctattact tgtggggatg
      721 ttttagtgga tagacaacag ggttgtacta tttggtccac ggagaagatt ttggcttttt
      781 tagctgatta ttttttgcag caaggagtta aggtaaacaa aattgttcac gtgactgaag
      841 tagcaggatt tttagataaa caaggtcaat tagttccaga gatcactgcc aaaacctggc
      901 ctaaattccg tcaggattta cgacatactc gaggttttga tgtcaccgga ggtatgggtt
      961 taaaaataga ggaatcactg aatttggcta agctaggtat tgaatcagta attatttctg
     1021 gcatgttgaa aaataactta tattgggctt taactaatca ggattgcgtg ggcacgacta
     1081 ttcgttagtt tttgcttccc aatcatctac cactgctttg caatagacat attggccata
     1141 ttcaaaggct cgctcgccca aatttttatc ggttgactcg ttagtttgta ccgattcact
     1201 aacaaattct tgagcaaatt ttccagcctc aatggctttt tgtgcactac ttttgatttg
     1261 ttctccagca ttagtactca attcattaag ttcgagattt aatttttgat cgctgtgaaa
     1321 agagttaact agatcctgcc agctgttgat ttcgccatct tggtattgat taatttgggc
     1381 ttgaagagga tgggttttta accaaccttt ttgataaaga aaaatactaa tagccagaag
     1441 aataaggaca ataattatta gtttgattat gagttttatt aatctgccga gtaatttcat
     1501 aattgattag accagtgcta agtaaaagtg ctaaatgaat ctgatttagc ctaatgttaa
     1561 ttttgatcgg gttgatcgag tgcgtgtgct aggactcgaa cctagaaccg ccaaggtata
     1621 agcttggagc tctaaccgat tgagctacac acgcgcttga tatgcattat agcaaacaac
     1681 tggagaaaac ttatttgcca attttctata aagttttctt ttctataaaa gcaacgcttt
     1741 atagaagcag tagtaataaa gctcctaagc caagaatgcc gaagccaact ttaatgaagt
     1801 taaaccagtc ggcaaaacca gattcggttg gtacagttac agctctttct atgacattac
     1861 cacc
//
LOCUS       DAOSEW010000095         1861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_63312_length_1861_cov_3.080842, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000095 DAOSEW010000000
VERSION     DAOSEW010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63312_length_1861_cov_3.080842"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="PLJ02_03440"
     CDS             <1..541
                     /locus_tag="PLJ02_03440"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HOI05487.1"
                     /translation="GYSSKIYDRNGELLYDLFQEERRQPSKAEEIPAVLKNATVAIED
                     RDFYKHSGFDFLTIIRIPYNLIFRKRVVGGSTLTQQLVKNALLTNERTIVRKFKELVL
                     AIQIERKFSKDEILTMYLNEAPYGGNIWGVATAVETYFAKPMTELTIVESAFLAGLPQ
                     RPSVYSPYSTRLDENGEPY"
     gene            545..>1861
                     /locus_tag="PLJ02_03445"
     CDS             545..>1861
                     /locus_tag="PLJ02_03445"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOI05488.1"
                     /translation="MRTRTVLKAMKDNAYITNEEYEQALKDIDSLTFNRTINEIKAPH
                     FVFYVQNQLEEMFGEELLLKGGLTVTTTLDWELQELAQTVVTEEVEDVARYDISNGAA
                     LAMQPQTGEILAMVGSKDYFDEAIGGQFNVAVDGLRQPGSAIKPITYLGMIKRGFTPA
                     TMIMDVPTTFARNEQDKPYTPKNYDGQFRGPVSLRQSLGSSLNIPAVKSLATVGLEEF
                     LTLAYEMGIETFEPTVDNLQRFGLALTLGGGEVYMLDLASAYSIFANGGLKVEPIAIL
                     KVTDSDGKVLFEHKQTQGKRVIEEGEAFLINHILSDNNARLLAFGPNSLLNTGRPIAV
                     KTGTTNDMKDNWTIGWSREILVASWVGNNDNSSMSYVASGITGASPIWRRIILGSLDM
                     GYAAPDWQIPDTVEQVEVDAISGYPSHDNFPSKFDYAIRGTVPSSPD"
BASE COUNT          542 a          334 c          434 g          551 t
ORIGIN      
        1 aggttatagt agcaagattt atgatcgcaa tggcgaattg ctttacgacc tgtttcaaga
       61 agaacgtcgc cagcccagta aggctgaaga aatcccagca gtacttaaaa acgcgacggt
      121 ggctattgag gaccgcgatt tttataaaca cagcggtttt gattttctaa cgattattcg
      181 tataccttat aatttaattt ttagaaaacg agtggttggt ggctctactc taactcagca
      241 gttagtaaaa aatgctttgt taactaatga gcggaccatt gtgcgtaaat tcaaagaact
      301 agtgctggca atccaaatcg agcgcaagtt tagcaaagat gaaatcttaa ctatgtatct
      361 taatgaagcg ccatacggag gtaatatttg gggcgtagcc actgcagtag aaacatattt
      421 tgctaagcca atgactgaat taaccatagt tgaaagtgcg tttttagctg gtttgccaca
      481 acgacccagt gtttattctc cctactctac tcgcctagat gaaaatggag aaccttatta
      541 gaaaatgcgg acgcgaacag tgctcaaggc gatgaaagac aatgcctata ttactaatga
      601 agaatatgaa caagcgctga aagatataga tagtctaact tttaatcgga cgattaatga
      661 gattaaagcg ccacattttg ttttttatgt ccaaaatcag cttgaagaaa tgtttggtga
      721 agagttattg cttaagggtg gtttaactgt tactaccact ttggattggg aattacaaga
      781 attagctcaa actgtggtta ctgaagaagt ggaggatgtt gctagatatg atattagtaa
      841 tggcgctgct ttagccatgc aaccacaaac tggtgaaatt ttggcgatgg tcggtagtaa
      901 agactatttt gatgaagcaa ttggtggtca atttaatgta gcggtcgacg gtttaagaca
      961 acctggctct gcgattaagc cgattaccta tttggggatg attaaacgtg gttttactcc
     1021 agccaccatg atcatggatg tgccaaccac atttgctaga aacgagcagg ataagcctta
     1081 cacacccaaa aattatgatg gccagtttcg tggtccagtc agcttgcgcc aatcactggg
     1141 tagttccctg aatattccgg cagtcaaatc cttagctacg gttggtttgg aagaattttt
     1201 aacgttggct tacgagatgg gtatcgagac cttcgaacct actgtagaca atttacagcg
     1261 ttttggttta gctttaactt tgggcggtgg tgaagtttat atgttggacc tagccagtgc
     1321 ttatagtatt tttgccaatg gcgggctaaa agttgaacca atcgcaattt tgaaagtaac
     1381 agatagtgat ggcaaagtcc tttttgaaca caagcaaact cagggtaaaa gagtgattga
     1441 ggaaggcgag gctttcttaa ttaatcatat tctttctgat aataacgctc ggttattggc
     1501 ttttggtcct aactctttac taaataccgg tcggccaata gctgtgaaaa ctgggaccac
     1561 taacgatatg aaggataact ggacaattgg ttggagcagg gaaattttag ttgccagctg
     1621 ggtgggtaat aatgataaca gttcaatgag ttatgtggct tctggtatca ctggggcttc
     1681 accgatttgg cgaagaatta ttttgggttc tctagatatg ggttatgctg ctcccgactg
     1741 gcaaataccc gacactgtag agcaagtgga agtggatgct atttctggtt atccaagtca
     1801 cgataatttc cccagtaagt ttgattacgc aattcgcggc actgtgccct cttctcccga
     1861 t
//
LOCUS       DAOSEW010000096         1824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_65232_length_1824_cov_3.754098, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000096 DAOSEW010000000
VERSION     DAOSEW010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65232_length_1824_cov_3.754098"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="PLJ02_03450"
     CDS             <1..885
                     /locus_tag="PLJ02_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05489.1"
                     /translation="MHILHPEEITQVRRIYDDEVWRYVTIPLTLADTQDWGRWQNFFY
                     QAYQQQLIPIIRLATRYDSTHDYWTIPNRKEIVTLISFLSSLNWYQQEKLVIVFNEVN
                     HAKEWGGKIDPLAYTYILKFTSNWLRSENQNFKILPAAMDLAAADRFPSMEAFNYLET
                     MYRLDPEIFNYIDYWNSHSYPNPGFSASPRNNGQNSLKGFTFELNYIKEKTGRDLPVF
                     ITETGWVSHYPTNFYLSDYYVYALEHVWSDDRIKAVTPFVLKGDPGPFATFSFFDFED
                     KPTIHTFALKRALKKLSP"
     gene            939..1009
                     /locus_tag="PLJ02_03455"
     tRNA            939..1009
                     /locus_tag="PLJ02_03455"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:971..973,aa:Gly,seq:tcc)
     gene            1056..>1824
                     /locus_tag="PLJ02_03460"
     CDS             1056..>1824
                     /locus_tag="PLJ02_03460"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05490.1"
                     /translation="MIDFLIFILAIFLLWLGAESVTKAAIKIAKTLSLSESFIGLTIV
                     AIGTSFPEIVISITGAVQNLRGEETSQIVLGNVIGSGMANLALILGILGIFKVIKLKK
                     NELLLDMLMLVLSTTILYLVSLDGLITIQDGVLLILFYLIYLLFLNRQNLRKQTILFK
                     KKQFKKKKLKKIKLAHLVQLALGLIILTKASQMVLDSGVILAEKLNINEMLVGILLVG
                     LGSSLPELMVSLNAALKGSMALSLNNLIGSNILNVFLA"
BASE COUNT          534 a          301 c          350 g          639 t
ORIGIN      
        1 atgcacattc tccacccaga agaaatcact caagtcagaa gaatttatga tgacgaggta
       61 tggcgttatg tcaccattcc gttgacttta gccgatactc aggactgggg ccgatggcag
      121 aatttttttt atcaagctta tcaacagcaa ttaattccta tcattcgctt agctactcga
      181 tatgactcaa cacatgatta ttggactatt cccaatcgga aagagatagt cactttaatt
      241 agttttctat ccagcctaaa ttggtatcag caagagaaat tggtgattgt ttttaacgaa
      301 gtgaatcatg ctaaagaatg gggcggtaaa attgatcctt tagcctatac ctacatcttg
      361 aaatttacca gtaattggtt acgtagtgaa aaccagaatt ttaaaatttt accggcagct
      421 atggatctag cggccgctga ccgttttcct agcatggaag ctttcaatta tttagaaact
      481 atgtatcgac ttgatccgga aatttttaac tacattgatt attggaattc tcattcttat
      541 ccaaatccag gattttctgc ttcacctagg aataatggtc agaattcgct aaaaggtttt
      601 acttttgaat taaattacat caaggaaaag actggtcgtg atttaccagt atttattacg
      661 gaaactggtt gggtatctca ttatccaact aatttttatt taagtgatta ttatgtttat
      721 gctttagagc acgtttggag cgatgaccgg attaaggcgg taaccccctt tgtgttgaaa
      781 ggtgatcctg gtccttttgc aacttttagt ttttttgatt ttgaagataa gccaactatc
      841 cacacttttg ccttaaaaag agcactaaaa aaattatcgc cttgatttgc ctaagcaatc
      901 ctttaaaata ttaactagtt tcttaaaatt tttttgctgc gggcgtagta catcggtagt
      961 atatctccct tccaaggaga agaggccagt ccgattctgg tcgcccgctc actctccctt
     1021 gattagtgac tgatatttct gctttaatgt aatagatgat cgatttttta atttttattt
     1081 tggcaatttt cttattgtgg ttgggagccg aatctgttac caaagcagct attaagattg
     1141 ctaaaacact gtccttgtca gaaagtttta ttggtttgac gattgtggcc attggtacta
     1201 gttttcccga aattgtgatt tctatcactg gggcagttca gaatttgcgt ggcgaagaaa
     1261 ccagccaaat tgtgcttgga aatgtgatcg gatcaggtat ggctaatttg gctctaattt
     1321 tggggatatt aggaattttt aaagtgatta aattaaaaaa gaatgagcta ttactcgaca
     1381 tgttgatgct agtgttgtct acgacgattc tttatctagt tagtctagat ggcttaatta
     1441 ctattcaaga cggagttttg ttaattcttt tttatttaat ttatttgttg tttttaaacc
     1501 gacaaaattt aagaaaacaa accatcttat tcaaaaaaaa gcaatttaaa aagaaaaagc
     1561 taaaaaaaat aaaattagct catctggtgc aattggcctt aggtttaatc atcctaacta
     1621 aggcttcgca aatggtttta gatagtggag taattcttgc tgagaaatta aatatcaacg
     1681 agatgttggt gggtattctg ctggttggtc taggtagtag tttgcctgaa cttatggttt
     1741 cgctgaatgc cgcgctcaag ggttcaatgg ctttatcttt aaataattta attggcagta
     1801 atatacttaa tgttttctta gcct
//
LOCUS       DAOSEW010000097         1672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_1795 NODE_74367_length_1672_cov_3.432282, whole genome
            shotgun sequence.
ACCESSION   DAOSEW010000097 DAOSEW010000000
VERSION     DAOSEW010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863324
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:32:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 695
            CDSs (total)                      :: 653
            Genes (coding)                    :: 650
            CDSs (with protein)               :: 650
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74367_length_1672_cov_3.432282"
                     /isolate="AMR_MDS_1795"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="PLJ02_03465"
     CDS             <1..484
                     /locus_tag="PLJ02_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI05491.1"
                     /translation="LGLVSYMGYLWVNFNDPFYFLTVQSQFGAGRQSQLVIWPQVVWR
                     YLKIIFTVRPIDWKYFAYVQEFCLSLIGLYYLIKAFLKKELPLAWILFALGAYFMPTL
                     TGNFSSMPRYILVCWPIFFYLAKDFSRWKPQAKILYLTISLLLLIINSMLFIQGYWVA
                     "
     gene            498..1415
                     /locus_tag="PLJ02_03470"
     CDS             498..1415
                     /locus_tag="PLJ02_03470"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOI05492.1"
                     /translation="MKILVTGGAGFIASHIVDAYVELGHQVVVIDNLSTGQKANLNPQ
                     AKFIECDITDQEKVQAIISSEQPAVINHHAAHIQVGYSVENPQFDAQNNILGLLNIMQ
                     AAKAVGVQKVIMASTGGAMYGNQATPFTEEMKEQPLSPYGISKRAGELYLNYYHEIFQ
                     IPFIVLRYSNVYGPRQNAHGESGVIAIFLEMIGEGKQPVINGDGTHTRDYVYVSDVVK
                     ANVLALDSDFVGELNIGTTVETSTNEVFDKLVAELGLDLQKEYGPERLGEQVTSALDY
                     SKAKRILGWEPTVDFAEGVKKTVEWFKKK"
     gene            complement(1451..>1672)
                     /locus_tag="PLJ02_03475"
     CDS             complement(1451..>1672)
                     /locus_tag="PLJ02_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011228271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HOI05493.1"
                     /translation="KHTIIAREYPKSKGEPYYPVPRARNSQVLAKYLAEAKELAKSGV
                     YFAGRLGTYRYLNMDQAVAEAMKLAEKIG"
BASE COUNT          474 a          340 c          358 g          500 t
ORIGIN      
        1 gttgggttta gtcagttaca tgggttattt atgggtcaac tttaatgatc ctttctattt
       61 tttgactgtg caaagtcaat ttggagcagg ccgtcaaagt caattggtca tttggcccca
      121 agtggtttgg cgctatctca aaattatctt cacagtcaga ccaatcgatt ggaaatattt
      181 tgcctatgtt caagaatttt gcttgagcct gatcgggctg tattatttaa ttaaagcctt
      241 tctaaaaaaa gagctaccct tagcttggat cttatttgct ctaggagcct atttcatgcc
      301 aactctaacc ggtaattttt ccagtatgcc gcgttatatc ttggtgtgtt ggccaatttt
      361 tttctatctg gctaaagatt ttagtcgctg gaaacctcaa gccaagatcc tctacctgac
      421 aattagtctg cttttactta taatcaacag catgctcttt attcaaggat actgggtagc
      481 ttaaatagaa aggtcagatg aaaatattag tcacaggcgg agcagggttt attgcttctc
      541 atattgttga tgcttacgtt gagttgggtc atcaagtagt ggtcatagat aatctttcaa
      601 ccggccaaaa ggctaatctc aaccctcaag ccaaatttat tgaatgtgac attaccgatc
      661 aagaaaaagt tcaagcaatc attagtagtg aacagccagc agtaattaat caccatgcag
      721 cgcatattca agttggttat tcggtggaaa atccgcaatt tgacgctcaa aataatattt
      781 taggcttatt aaacatcatg caagccgcta aagcagtcgg tgtgcaaaaa gtgatcatgg
      841 cttccactgg tggtgccatg tatggcaatc aagccacacc atttacagaa gagatgaaag
      901 agcaaccact gtcgccttat ggcatttcca agcgtgctgg cgaactgtac ctcaattact
      961 atcacgaaat ttttcagata ccattcattg ttttgcgtta cagtaatgtc tatggccccc
     1021 gccaaaatgc ccatggtgag tctggagtaa ttgccatctt tttggaaatg atcggtgagg
     1081 gcaaacaacc agtgatcaat ggtgacggca cccacactcg agattacgtt tacgtcagcg
     1141 atgtggttaa agccaatgtt ttagctctgg atagtgattt tgtcggtgaa ttaaatattg
     1201 gtaccaccgt cgaaacctcg accaatgaag tgtttgataa attagtggca gaacttggcc
     1261 tagatctaca aaaagaatat gggccagaac gcctgggtga gcaagtaact agtgcccttg
     1321 attacagtaa agccaagcgg attctgggtt gggaaccaac agttgatttt gctgaagggg
     1381 tgaaaaagac ggtggaatgg tttaaaaaga aataattgat cggttaccac tcggcaattt
     1441 acaaatgacc ctagccaatt ttttcagcca acttcatcgc ttccgccacc gcctgatcca
     1501 tatttaaata gcgataagtc cctagtcttc cagcaaaata taccccagac ttggccaact
     1561 ctttagcctc agccaaatac ttagccaaaa cttggctgtt gcgggctctg gggacgggat
     1621 aatagggctc gcctttgctt ttaggatatt ctctagcaat gatggtgtgt tt
//
